LOCUS       DBNHVN010000001         1069 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1931947, whole genome shotgun sequence.
ACCESSION   DBNHVN010000001 DBNHVN010000000
VERSION     DBNHVN010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1931947"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1069)
                     /locus_tag="AC16Y1_00005"
     CDS             complement(<1..>1069)
                     /locus_tag="AC16Y1_00005"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR5830882.1"
                     /translation="AELAAKGSSVSDEDLDKRRTHAGPDDTATIIYTSGTTGMPKGCV
                     LTHANLFAEVDNLVEGIPEVFKAPQASTLIFLPLAHVFGRLIQVAMIRGQVTIGHCPN
                     PVALLKDLESFKPSFLLAVPRVFEKVFNGAGAKAHAASPVKGKIFDRAAATAIAYSEA
                     LDQGKPSAGLSIKHKLFDKLVYSKLRAAMGGRVTHAISGGAALGTRLGHFYRGLGLIV
                     LEGYGLTETTAGSTLNIPSALKIGSVGKPVFGTGIKIADDGEVLIKGPHVFKEYWNNE
                     LATKEVFTEDGWFKSGDIGELDDEGFLRITGRKKELLVTAGGKNVAPAVLEDRLRAHP
                     IVSQCVVVGDGQPYIGALVTLD"
BASE COUNT          297 a          260 c          229 g          283 t
ORIGIN      
        1 ggtccaatgt gactagagca ccgatgtatg gctgaccatc gccaacaacg acacattgac
       61 taacaatggg atgtgcacgc aatctatcct cgagaactgc aggagctaca ttcttaccgc
      121 ccgcagttac taggagttcc ttcttgcgac ccgtgatgcg aaggaagcct tcgtcatcta
      181 actcgccaat gtcaccagat ttgaaccagc catcctcagt gaacacttct ttagtggcta
      241 actcgttatt ccaatattct ttaaatacgt gtggcccctt aataagaact tcgccatcgt
      301 cagcaatctt tattccagtt ccaaatactg gtttaccgac actgccaatt ttcagtgctg
      361 agggaatatt taaagttgaa ccggctgtgg tttctgttaa gccatagcct tctagcacaa
      421 ttagtcctaa cccacgatag aagtgaccta agcgtgtgcc gagtgccgca ccaccagaaa
      481 ttgcatgcgt aacgcgacca cccatagctg cacgaagctt tgaatagaca agtttgtcga
      541 ataatttgtg cttgattgaa agtccagcag atggtttgcc ctgatctagc gcttcactat
      601 aagcaattgc ggttgctgca gcacgatcga aaatttttcc tttaactgga ctagcagcat
      661 gtgcttttgc accagcacca ttgaatactt tttcaaatac tcgcggcacc gccaaaagga
      721 aacttggttt aaaagattct aaatccttaa gtaaagcaac gggatttgga caatgtccaa
      781 tagttacttg gccacgaatc atggcaactt gaattaagcg accaaaaaca tgtgcaagtg
      841 gaaggaaaat tagagtcgaa gcctgcggtg ctttgaaaac ctcggggata ccttcaacta
      901 agttatcgac ctcggcaaat agatttgcat gggttagtac acaacctttt ggcatcccgg
      961 tagtaccact ggtgtaaata attgtggcag tgtcatcagg acctgcatgt gtgcggcgct
     1021 tatctaagtc ctcgtccgac acactcgagc ctttagcagc aagttcagc
//
LOCUS       DBNHVN010000002         1966 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_371817, whole genome shotgun sequence.
ACCESSION   DBNHVN010000002 DBNHVN010000000
VERSION     DBNHVN010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_371817"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="AC16Y1_00010"
     CDS             complement(<1..264)
                     /locus_tag="AC16Y1_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007382357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008235 - metalloexopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HIR5830883.1"
                     /translation="MLRISQNLVDQMVAHARADHPDEACGVIAGPANSDRPERFIPMI
                     NAARSPTFYEFDSADLLALYKDLDAREEDPVVIYHSHTATQAYP"
     gene            complement(284..793)
                     /locus_tag="AC16Y1_00015"
     CDS             complement(284..793)
                     /locus_tag="AC16Y1_00015"
                     /inference="COORDINATES: protein motif:HMM:NF020989.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 domain-containing protein"
                     /protein_id="HIR5830884.1"
                     /translation="MFRQTYEGKVTLDLSSDERALLIDLFTQMQNLLETPDVHENSDP
                     LAALVGLDGPTQEPTDPAIARLFPNAYLDDEQAAQDFRRYTEPDLRSEKMQNLDLVSD
                     SLAQDSQSLELTSEETGAWLKSLNDLRLVLGTRIGVSNEYQEDQSDDPGFHLYDYLTY
                     LQGSLIDAL"
     gene            complement(795..1073)
                     /gene="clpS"
                     /locus_tag="AC16Y1_00020"
     CDS             complement(795..1073)
                     /gene="clpS"
                     /locus_tag="AC16Y1_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HIR5830885.1"
                     /translation="MSVAPIQLADVDVATARPWVTIVWNDPINLMTYVTFVFQDYFGY
                     DKPKAEKLMLDVHHKGKAIVSSGSREEMERDVAAMHSYGLWATTQRDI"
     gene            complement(1111..1383)
                     /locus_tag="AC16Y1_00025"
     CDS             complement(1111..1383)
                     /locus_tag="AC16Y1_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HIR5830886.1"
                     /translation="MTSEEFRTEGGLLVDEKTEQQTQAGDHERFAHYVKKDKIVESAV
                     LGGAVVALCGKVWVPSRDPKKFPVCPECKEIFEGLPEGKKNKGSDS"
     gene            complement(1406..>1966)
                     /locus_tag="AC16Y1_00030"
     CDS             complement(1406..>1966)
                     /locus_tag="AC16Y1_00030"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010156513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HIR5830887.1"
                     /translation="RATNVPLVAQSFSWNHGVFMGATVSSEMTAAAVGGVGQLRRDPF
                     AMLPFCGYNMADYWSHWIEMGENFSALPKIFQVNWFRKDKDGKFIWPGFGENSRVLAW
                     IVDRLEGHVDGIESPLGILPITDDLNLAGLELSADNLAELFRIEKESWLEECSLTDEY
                     FDMFGDRIPDELEAELTALKQRLTSA"
BASE COUNT          548 a          466 c          397 g          555 t
ORIGIN      
        1 tggataagcc tgcgtggctg tgtgtgaatg gtaaataaca acgggatcct cttcgcgagc
       61 atctaaatct ttatacagcg ccaatagatc tgcggaatcg aattcataaa aagttggtga
      121 tcgagcagca ttgatcattg ggatgaatcg ctcaggtcgg tctgaatttg cggggccagc
      181 aataactcca cacgcttcgt ccggatggtc agcgcgcgca tgggcaacca tttgatcaac
      241 taaattctgg ctaattctga gcatcgcccc tccctaatag atcttagagg gcgtcaatta
      301 gtgagccttg tagataagtc aagtaatcgt ataagtgaaa accaggatca tccgattggt
      361 cttcttgata ctcatttgaa actccaattc gagttccaag tactaaccgc aagtcattca
      421 atgacttaag ccacgcacca gtctcttcac tagtcagttc caaactttgt gaatcttgcg
      481 ccaacgaatc tgaaactaga tcaagattct gcattttttc tgaacgcaag tctggctcgg
      541 tgtatcgacg aaaatcctgc gcagcttgct cgtcatctaa ataagcattt ggaaataacc
      601 gggcaatagc tggatcagtt ggttcttggg ttggaccatc aagtccaact aaagcagcga
      661 gtgggtctga attctcgtgg acatctggtg tctccaataa attttgcatc tgggtaaata
      721 aatcaataag taaagcccgt tcatctgaag acagatctaa tgtgactttt ccttcgtaag
      781 tttgccgaaa catttcagat atcccgctga gtggtagccc atagtccgta agagtgcatt
      841 gctgcaacat cacgttccat ttcttcacga gatccgctac ttacgattgc tttacccttg
      901 tggtgaacat caagcattaa cttttctgct tttggtttgt cataaccaaa ataatcctga
      961 aatacaaaag tgacatacgt cattaagtta attggatcat tccaaacaat ggtcacccac
     1021 ggacgagcag ttgctacatc aacatctgct agttgaatcg gggcaacgct cacacttgta
     1081 gtttgccacg caaactaggc aagtaaaagc ctaggaatca gaacctttat tttttttgcc
     1141 ttcaggtaaa ccttcaaaaa tttctttaca ttcggggcaa accgggaatt tttttggatc
     1201 tctgcttggc acccaaactt ttccacaaag agcaactact gcgccaccta aaactgcact
     1261 ttctacaatt ttgtcttttt taacataatg cgcgaaacgt tcatgatcac cggcttgtgt
     1321 ttgctgctcg gttttttcat caactagtaa gccaccttcg gtacgaaact cttcagaagt
     1381 catgtcgtca gtttaaccaa cttagttatg cgctggttag gcgttgtttg agcgcagtta
     1441 actcagcttc aagttcatct gggatgcgat caccgaacat gtcaaagtac tcatcagtta
     1501 acgagcactc ctcaagccat gactcttttt ctattcggaa aagctcagct agattatccg
     1561 ctgacaattc caggccagca agatttaaat cgtctgtgat tggcaaaata ccaagtggtg
     1621 actcgattcc atcaacatga ccttcgagtc gatctacgat ccaggcaagt acccgcgagt
     1681 tttcaccaaa acctggccag ataaatttgc cgtctttgtc ttttctaaac cagttgactt
     1741 ggaaaatctt tggcaaagct gagaaatttt cacccatctc aatccaatgg ctccaataat
     1801 ctgccatgtt gtaaccgcag aatgggagca ttgcaaatgg gtcacgcctt aactgtccaa
     1861 ctccgccaac agcagctgca gtcatttccg atgagacagt tgcacccatg aagacgccat
     1921 gattccaact aaatgattga gccactagcg gaacattcgt agcacg
//
LOCUS       DBNHVN010000003         5545 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_297732, whole genome shotgun sequence.
ACCESSION   DBNHVN010000003 DBNHVN010000000
VERSION     DBNHVN010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_297732"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="AC16Y1_00035"
     CDS             complement(<1..1251)
                     /locus_tag="AC16Y1_00035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HIR5830888.1"
                     /translation="MRNQELMSQASHLVAGEKPLTHSEILKVLSGLMMGLFLAALDQT
                     IVSTALKSIVEDFNGLNHYTWVVTAYLLTSTASTPLYGKISDLYGRRPVFQFAIITFL
                     LGSLLAGTAQDMTQLIAFRAIQGLGAGGLMALTFVIIGDIIPPRERGRYQGYFGAVWG
                     LSSVAGPLLGGYFADAGQIFGVSGWRWIFYINLPIGVLALFLTTASLHVHNVKKKHKI
                     DYFGAFLMISGVTSLLMGVSVYGPQDGWTDPKTDIALGLAVLLTLAFLLQERRAVEPI
                     LPLHLFKNHTFSITSLIGFVIGAGMFGAIIMIPLYLQLVVGDSATDAGLKLIPFMLGI
                     VSMSMYSGKQISKKGHYKRFPIIGLIIMTVGLALLVRLTETTSFTELAIYEFMIGAGL
                     GLSMQPIVIALQNDLDFKDMGIGTS"
     gene            complement(1333..1992)
                     /locus_tag="AC16Y1_00040"
     CDS             complement(1333..1992)
                     /locus_tag="AC16Y1_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5830889.1"
                     /translation="MDGRYVLIVEDDALLRELLATALESWGYSVQTASNAADAKRLFK
                     MSDPDGVILDIDLGPGPNGFDLAHIILESAPATSIVFLTNMPDPRFAEVDPDGLPTGI
                     AYLRKSALSDLNLLSSALDATIRGEVTNELRHDRDKNRPFANLTKKQIEVLRLMSQGK
                     SNAQIAQIRGTSVKAVEDAIRRSCEAIGVDNSVDGNTRTAAVAKYLSVTGMIKPISVR
                     N"
     gene            2163..3362
                     /locus_tag="AC16Y1_00045"
     CDS             2163..3362
                     /locus_tag="AC16Y1_00045"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR5830890.1"
                     /translation="MGKNQMSRIAHSVLMRTGLILSLVLISATITSCSTSSGETENAA
                     FSTPEPANSILIEPTSFTVVTTGDILLHERTWNQAKQDGTEEIWNFYPQLEGIASVTQ
                     NADLALCHLETPIANKNESYSGYPVFNSPPEILYAVKKLGFDMCGVASNHSLDKGFTG
                     IERTLNKLDQIGIAHAGIARTQAEADTPLIVDVQVNGKTFKVGIVAYTYGFNGFNRDA
                     DKLWSANLIDTQQIINEAKAARHDGAQFVIAKLHWGDEYTNKPNTYQTELAQELAQSG
                     VIDLIDGAHSHSVQPITQIENMWVAYSHGNLIAAHREPTTIKSEGLIVRWTISENVDG
                     RLKISKVEQIPILITDSFPVRVINVPKALADGDFSLANKSRLTQAFERSVETVNSLGF
                     EIPVGQE"
     gene            3407..3619
                     /locus_tag="AC16Y1_00050"
     CDS             3407..3619
                     /locus_tag="AC16Y1_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002655533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HIR5830891.1"
                     /translation="MSEEKATITVRPNGSLRVNGATLIGADGQVITNKETYSLCRCGQ
                     SADKPFCDGAHRGCEFTDEGTHSPVE"
     gene            complement(3788..4507)
                     /locus_tag="AC16Y1_00055"
     CDS             complement(3788..4507)
                     /locus_tag="AC16Y1_00055"
                     /inference="COORDINATES: protein motif:HMM:NF033747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HIR5830892.1"
                     /translation="MGKHRRLNVKRYSALLLIGELLITAGFVLGSYVAYELWVSNFTA
                     QRTWTNSSKDLTEEFSAEFKNYLAENPIPPEKIEITQTPKSGKPFGLMYFPKLWASEK
                     VVPIITGISDSDLAKGLGYYPNTSLPGEVGNFAVAGHRATHGEPFADFPLIEVGDEVI
                     VETFAGRYVYELVDDIKVKPEDVWVIGSRPNVPGVMALPADSKLITLTTCDPRWSSEN
                     RWIWFGVQKSFTSRYELERRT"
     gene            4636..>5545
                     /locus_tag="AC16Y1_00060"
     CDS             4636..>5545
                     /locus_tag="AC16Y1_00060"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HIR5830893.1"
                     /translation="MKLVVPTETKSGEKRVALVPEAVAKLVKLGLKVSVQSEAGAGAT
                     YSDNEYVKAGAKIFTKAALTRELASANLVASVRPLPTRQISTLKKNTITLSFLSPVNN
                     TTEIKALAKVGVTALSFDLVPRISRAQSMDALTSQALCGGYRATLIAAEKSTKFFPML
                     MTAAGTVRPAKVLVLGAGVAGLQAMATAKRLGAVVSAYDVRSSSADEVKSMGATFINL
                     GLDALEGSGGYAKEMNKDRTKAQQDALKPYVSESDIVITTAAVPGKKAPLLITKDMMS
                     QMKPGSVLVDLAAETGGNIQGTIAGKD"
BASE COUNT         1712 a         1180 c         1157 g         1496 t
ORIGIN      
        1 gcttgtgcca atacccatat ctttgaagtc caggtcgttt tgcaaagcaa ttactatcgg
       61 ttgcattgaa aggccaagcc cggcaccaat catgaattca tatatcgcca actcagtaaa
      121 tgacgtagtt tctgtaagtc ggactaataa tgccaaacca acagtcatga tgatgaggcc
      181 gatgattggg aagcgtttgt aatgaccttt tttggaaatt tgctttccgg aatacataga
      241 catcgagacg atacccaaca taaacgggat taactttaat ccggcatccg tagcggaatc
      301 ccctacaact aattgcaggt aaagcggaat cataatgatt gcgccaaaca ttccagcacc
      361 aataacaaat ccaattaatg aagtgattga gaatgtgtga tttttgaata aatgcagtgg
      421 caagattggt tctaccgcac gacgctcttg caataagaat gcaagggtaa gtaacacagc
      481 taagccaagt gcgatatcag tttttggatc agtccaacca tcttgtgggc cataaactga
      541 aacgcccata agtaaagatg tgacgccaga aatcattaag aatgcaccga agtaatcaat
      601 cttgtgtttc ttcttgacat tgtgaacgtg taacgaggca gtagtaagaa atagcgccaa
      661 aacaccaatc ggcaaattaa tgtaaaaaat ccagcgccaa cccgagactc caaaaatttg
      721 gccggcatcc gcgaagtaac caccaagtaa tggtccagca actgaagata atccccagac
      781 agctccgaaa tatccttgat aacgcccacg ttcgcgcggc ggaatgatat caccgatgat
      841 tacgaaagtt aacgccatta atccaccagc acccaaacct tggattgcac ggaatgcaat
      901 tagttgagtc atgtcttgcg cagttccagc taaaagcgaa cctaataaga atgtgattat
      961 ggcgaattga aaaactggac gacgaccgta taagtctgaa atttttccat atagcggagt
     1021 tgaagcggtg gaggtcagca agtacgcagt gacgacccag gtgtaatgat ttaatccgtt
     1081 aaagtcttca acaatacttt ttagtgcggt tgaaacgatt gtttgatcta aggcagccaa
     1141 aaataaaccc atcatcaatc cacttaaaac tttaaggatt tctgaatggg ttaagggttt
     1201 ttcaccggcg acaagatggc tagcttgaga cattaattcc tgatttctaa tttgagtgct
     1261 tgtttagttt agtccgccgt caaggttaat caagaaagct aggtttgaaa ttttcgcagt
     1321 tagtgaaatc tattagtttc gaactgagat tggcttaatc attcctgtta ctgataagta
     1381 cttagccaca gctgctgtac gtgtatttcc atcaacactg ttatcaacac ctattgcttc
     1441 acatgatcgc cggattgcat cttctacagc ttttacagat gtgccgcgta tttgtgcaat
     1501 ttgagcgttt gattttcctt gtgacatcaa tctaagtact tcgatttgtt tcttagttag
     1561 atttgcaaat ggccgattct tgtcacgatc atgccttaac tcgtttgtaa cctcaccgcg
     1621 gatagttgca tccaatgcac ttgataaaag atttaaatca gacaaagcag actttcgcaa
     1681 ataagcaatt cctgtaggca gtccatccgg atcaacttca gcaaatctgg gatctggcat
     1741 gtttgttaag aacacaattg aggtggcagg tgcgctttct aaaataatgt gcgctaaatc
     1801 aaaaccgttt ggccctggtc ccaaatcaat atcaagaatt acaccgtctg gatcgctcat
     1861 tttaaacaaa cgcttggcat cggcagcatt gctcgcggtt tgtacagagt aaccccagga
     1921 ctcaagtgca gtagctagta actcacgaag tagtgcgtca tcttctacga ttaggacgta
     1981 gcgtccatcc atttaataac ctgcttaaat tatgaaaagt ttcttagtta taggattact
     2041 catataagta aggtacttat cccttttagt ggggaaaacc cccttaaatt tcctgactga
     2101 aggcatcaat ttctgcattt tatttgggtt actcctaaat ttggtgcagg gaaataagtc
     2161 tgatgggtaa gaatcaaatg tctcgaatag cgcattcagt attaatgcgc acagggctaa
     2221 tcctgtccct tgtcctaatc agcgcgacta taaccagttg ttcgacaagc tcaggtgaaa
     2281 ctgaaaacgc cgctttcagt acaccagaac ctgctaactc aatcttgatt gagcccacga
     2341 gtttcactgt tgtgacaacc ggcgacattt tgttacacga aagaacttgg aatcaagcga
     2401 aacaagacgg aactgaagaa atctggaatt tctatcctca acttgaaggc atcgcatctg
     2461 tcacccagaa tgcagatcta gccttgtgcc atcttgaaac cccgatcgca aacaaaaatg
     2521 aaagctactc aggttaccca gttttcaatt cgccgccaga aattctttat gcggtgaaga
     2581 aacttgggtt tgacatgtgc ggtgttgctt caaaccattc actagataaa ggttttaccg
     2641 gaatagaacg aacgttaaac aaactggatc agatcggcat tgcacatgcc ggaatagcca
     2701 gaactcaagc ggaagcagat acgccactaa ttgttgatgt gcaggtaaat ggaaaaactt
     2761 ttaaagtcgg aatagttgcc tacacctatg ggttcaatgg tttcaatcgt gatgccgata
     2821 aattatggag tgccaattta atcgatacgc agcaaattat taacgaagca aaagcagcac
     2881 gacatgatgg tgcccaattt gtgattgcaa aattacattg gggggacgaa tacaccaaca
     2941 aaccaaacac ttatcagact gagctggcac aggaacttgc ccaaagcgga gtcattgatt
     3001 taattgatgg tgcccactcg cactcggttc aaccgataac tcaaattgaa aatatgtggg
     3061 tcgcttatag tcatggcaat ttaattgcag cacatcggga accgacaact attaaatccg
     3121 aaggcctaat cgtgcgctgg acgattagtg aaaatgttga cgggagattg aaaatttcta
     3181 aagttgaaca aattccaatt ttgattaccg atagttttcc ggtgcgagta ataaatgtgc
     3241 ctaaagccct agcagatggt gatttcagtt tggctaataa atcaagactt actcaggctt
     3301 ttgaacggtc tgtcgaaact gttaactcgc ttggtttcga aataccagtg ggtcaagaat
     3361 aaattttccg cgtacgatta agttaaccaa agcgatttga ggaaaaatgt cagaagaaaa
     3421 agccacaatt accgttcgcc ctaatggatc tttacgggtt aatggagcaa cattaattgg
     3481 cgctgatggt caggtcatca caaataaaga gacttattcg ctttgccgtt gtggtcagtc
     3541 tgccgataaa cctttttgtg atggagcgca tcgcggttgc gaatttaccg atgagggcac
     3601 acacagtccc gtcgaataag tgattactaa ctttcggaaa atgtcggatc acccgaaaaa
     3661 aacgaattca catttgggaa aactaaaaac caaagtatga ataacgccaa ggtaatcaaa
     3721 agaaatgcct gtagccattt aaaccacttt ggtccacgca gttttcgcca aatccagcta
     3781 taaatcatta ggttcgcctt tcaagttcat atctagaagt gaaagacttt tgaacaccga
     3841 accaaatcca gcgattttca gatgaccaac gtggatcgca agttgtcaga gtaattaatt
     3901 ttgaatccgc tggtaacgcc atgacaccag gtacattcgg cctggaacca ataacccaaa
     3961 catcttctgg cttaactttg atgtcatcaa ctaattcgta gacatacctg cccgcaaaag
     4021 tttcaacaat aacttcatcg ccaacttcaa tcaacggaaa gtcggcaaat ggttcaccat
     4081 gggttgctcg atgaccagct acggcaaaat ttccaacttc accaggtagt gaagtgttcg
     4141 ggtagtagcc aagacctttt gctaaatcgc tatctgaaat accggtaatt attggaacca
     4201 ctttttcgct agcccataat tttggaaaat acattaagcc aaaaggttta ccagattttg
     4261 gtgtctgagt tatttcaatt ttttctggtg gtatcggatt ttctgctaaa taatttttaa
     4321 actcagctga aaattcttct gttaaatcct ttgaagaatt agtccaggtc cgctgcgctg
     4381 taaaattact gacccaaagt tcgtaagcta cataagaacc caaaacgaaa cctgcagtaa
     4441 taagtaactc accgatcagc aaaagtgctg agtaacgctt aacgtttagc cttcgatgtt
     4501 tacccacgta acaagactat ttggtgaaat gcggatgcta tttaacgcat gccttgtaac
     4561 taaacttcca tcaaaaagac agagttcaat tagcgctcta gagttgtact aagaaattgc
     4621 cctttggaga gccttgtgaa acttgtagtt cctactgaaa ctaaatctgg tgaaaagcga
     4681 gtggctttag tgcccgaagc ggttgccaaa ttggtcaagc ttggtttaaa agttagtgtc
     4741 caatcagaag ctggtgctgg tgccacatat tcagataatg agtatgtaaa agctggtgca
     4801 aaaattttta ctaaagctgc actgactcga gaactggctt cagcaaatct agttgcatct
     4861 gttcgaccat taccgacccg gcaaatttct acattaaaga aaaacacgat cacactctct
     4921 tttttgtcgc cggtaaataa caccactgaa attaaagcgc tagcaaaagt cggggtaacc
     4981 gcgttgtcgt tcgacctggt acctcgtatt tctcgtgcgc agtccatgga tgcgctaact
     5041 tctcaggcac tctgtggtgg ataccgtgca acattaattg ccgctgaaaa atcaacaaag
     5101 tttttcccga tgctgatgac cgcagctgga actgtccgcc cagcaaaagt gttggtactg
     5161 ggtgccggtg ttgctgggtt acaagcaatg gcaactgcaa aaaggctcgg tgcagttgtt
     5221 agtgcctacg atgttcgctc atccagcgct gatgaagtta aatcaatggg cgcaacattt
     5281 atcaacttag gtcttgatgc tctcgaaggc agcggcggtt atgccaaaga aatgaataaa
     5341 gatcgcacta aagctcagca agatgcatta aaaccatacg tttcagaatc tgacatcgta
     5401 atcacaactg cagctgttcc tggaaaaaaa gcaccattac tcataaccaa agacatgatg
     5461 tcgcaaatga aacctggatc tgtcctagtt gatctagctg cagaaacagg tggaaacatt
     5521 caaggaacta ttgccggcaa ggacg
//
LOCUS       DBNHVN010000004         1793 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_75257, whole genome shotgun sequence.
ACCESSION   DBNHVN010000004 DBNHVN010000000
VERSION     DBNHVN010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_75257"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="AC16Y1_00065"
     CDS             <1..222
                     /locus_tag="AC16Y1_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HIR5830894.1"
                     /translation="VLAGNLAGLSVGGAAVLGVLSASASYIAAPAAVAVGLPKANTSL
                     ALMASIGVTFPFNLVFGIPLYLEFANWLG"
     gene            227..481
                     /locus_tag="AC16Y1_00070"
     CDS             227..481
                     /locus_tag="AC16Y1_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5830895.1"
                     /translation="MESEEFEGESVLDVEIEELDEDLAQGPSKPAIALSTLPITGEPR
                     VDDALARLNEIPGLPVHEHVEVFEDVQRRLHETLADLSNQ"
     gene            491..1282
                     /locus_tag="AC16Y1_00075"
     CDS             491..1282
                     /locus_tag="AC16Y1_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020544746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR5830896.1"
                     /translation="MVRRRLDVELVRRHLARSREQAQEYIDAGFVEVNGMVASKSASQ
                     VEENAAIRVNTDPDVDYVSRGAHKLIGALDAFGLDVTGKVILDAGASTGGFSDVLLRR
                     GAAHIYCVDVGYGQLAWKIANDPRVKVIDRFNVRNLTEADLGQCVDLIVADLSFISLE
                     TVLPALVGVLKVSGDALLMVKPQFEVGKELLGPGGVVTDNSLRIDAVNKIANKAFELG
                     LGMAGVVASPLPGPSGNVEYFLWLNRSASAPQLTSIEKAVQEGPQ"
     gene            1279..>1793
                     /locus_tag="AC16Y1_00080"
     CDS             1279..>1793
                     /locus_tag="AC16Y1_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013663.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR5830897.1"
                     /translation="MSRKVLFVVHNARNEAVEVASRAAKGLLAKNIEVLVTENYSGNL
                     DSAGVKIASDAKGCELVIVFGGDGTILRGVEIARESNVPVLGVNLGHVGFLAEAEPED
                     LETVIDVVVNGSWTVEERTALSVAVVGQDGAKWNSWALNEISIEKAQRERMATLLVSI
                     DGQPVSKWNCDG"
BASE COUNT          449 a          344 c          486 g          514 t
ORIGIN      
        1 gtattggccg gtaatttggc tggcttatct gttggtggcg cagcggtact tggcgtactt
       61 agtgcaagtg cctcttatat agcggcgccg gcggcagtag ctgttggctt accaaaagcg
      121 aatacttcac ttgctctgat ggcaagtatt ggtgtgacct tcccatttaa cttggtgttt
      181 ggtattccgc tttatctaga atttgccaac tggttaggct agttacatgg aatctgaaga
      241 attcgaaggc gaatcagttc ttgatgttga aatcgaagaa cttgacgaag atttagcaca
      301 aggtccaagt aaacctgcga tcgcactgtc gactttgccg attactggcg aacctcgcgt
      361 tgatgacgca ttggctcgcc ttaacgagat tccgggctta ccagtacatg aacatgttga
      421 agtttttgaa gacgtgcagc gtagattgca cgaaactttg gccgatcttt cgaatcaata
      481 ggtctattac ttggttcgtc gacgcttaga tgttgaatta gtaaggcgtc atttagctcg
      541 tagccgtgaa caggctcaag aatatatcga tgccggtttt gttgaagtca acggaatggt
      601 ggcaagtaaa tcggctagcc aagttgagga aaatgctgca atccgggtta acactgatcc
      661 tgatgttgat tacgtttcgc gcggagcgca taagttaatt ggcgcactcg atgcttttgg
      721 tctagatgtc actggcaaag tgattttaga tgcaggtgcc tcaactggtg gcttttcaga
      781 tgttttactt cgccgaggcg cggcacatat atattgcgtt gatgttggct atggtcaatt
      841 agcctggaaa attgccaacg atcctcgagt taaagtcata gatcggttta atgtgcgaaa
      901 cctgaccgaa gccgacttag gccaatgtgt tgatctgatc gtggcggact tatcgtttat
      961 ttcacttgag accgtgttac ccgctctcgt cggtgtgctc aaagtaagcg gggatgcctt
     1021 attaatggtt aaaccccagt tcgaagttgg taaagaatta cttggcccgg gtggggtagt
     1081 tactgataat tcgttgcgca ttgatgcagt caataagatt gccaataagg cgtttgaatt
     1141 aggccttggt atggctgggg ttgttgctag tccgctaccg ggaccttcag gaaatgttga
     1201 atatttcttg tggttaaacc gaagtgcttc ggcacctcaa ttaacttcaa tcgaaaaagc
     1261 tgtgcaggaa ggaccgcaat gagtcgaaaa gtactctttg ttgttcataa cgcacgcaac
     1321 gaagcagttg aagtagcaag tcgcgcagct aaaggattac ttgctaaaaa cattgaagtt
     1381 ttagttactg agaattattc gggcaactta gattcggctg gcgtgaaaat tgcatctgat
     1441 gccaaaggtt gcgaactggt aatcgtgttt ggcggtgacg gcactatttt gcggggcgtt
     1501 gaaattgccc gcgaaagtaa tgtgccagtg cttggcgtga acttaggcca tgttgggttc
     1561 ttagccgaag ccgaacctga agatttagaa actgtgatcg atgttgtggt gaatggctcg
     1621 tggacagttg aagaacgtac cgcgctttcg gtggcagttg ttgggcaaga tggggccaag
     1681 tggaattcat gggccctgaa cgaaatttca attgaaaaag cacaacgtga acgcatggct
     1741 accttattag taagtattga tgggcaacca gtatctaaat ggaattgcga tgg
//
LOCUS       DBNHVN010000005         1840 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_521096, whole genome shotgun sequence.
ACCESSION   DBNHVN010000005 DBNHVN010000000
VERSION     DBNHVN010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_521096"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /gene="rsmI"
                     /locus_tag="AC16Y1_00085"
     CDS             complement(<1..725)
                     /gene="rsmI"
                     /locus_tag="AC16Y1_00085"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR5830898.1"
                     /translation="MSGQLILAATPIGNSADASPRLCEALETADIIAAEDSRRAKRLL
                     QDLGISTNAQIWSFYDAVEESKSPELIERVQAGNKVLVISDAGMPTVSDPGYRIVQAA
                     IESGVAVSVIPGPSAVLTALAVSGLSVDRFTFEGFLPRKSGEREKFLDQLVNETRTMV
                     FFEAPHRLVEALEDFAKVFGSDRQAVVCRELTKTYEEVVRGSFAELLNWAQQEILGEI
                     TLVVAGSKPVLVTDPVQWVELVQT"
     gene            781..>1840
                     /locus_tag="AC16Y1_00090"
     CDS             781..>1840
                     /locus_tag="AC16Y1_00090"
                     /EC_number="2.4.1.109"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004169 -
                     dolichyl-phosphate-mannose-protein mannosyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate-mannose--protein
                     mannosyltransferase"
                     /protein_id="HIR5830899.1"
                     /translation="MSSTQLETTQKPAQFDLDIKKPMAGGIRGWFGPLAATVVAAALR
                     LPNLGTPHALVFDETYYAKDALSLLNFGVEKKFVDGANEKLLASDGQNYLDIFLNQPA
                     YVVHPPIGKWIIASGEAIFGATPFGWRIAVAILGILSVLLTARITRRLTNSNFIGTVA
                     GFLLALDGLHIAMSRTALLDTPLSFFVLCAFGALILDRDRKTNESFRYWRWIMIGFLG
                     LAIATKWSGLYFAAAFGLLMLIWDLNRRTKISQWLKKDLLPAILMPFVALAIYIASWV
                     GWFASSDGWARNFAADKNNSFLPESLVSLFQYHKDMWSFHTNLTTAHNYEANPWGWPI
                     LYRPTSFFYDTASICGGPS"
BASE COUNT          495 a          449 c          379 g          517 t
ORIGIN      
        1 gtttgcacta gctcgaccca ttgcactgga tcagtaacta atactggctt actacctgcc
       61 accaccaaag taatttcacc aagtatctct tgctgggccc aattcaaaag ctcagcaaaa
      121 ctgccgcgca caacttcttc gtaagttttc gttaactcgc gacaaaccac ggcttgtcga
      181 tctgaaccga atactttggc aaaatcctca agtgcttcaa ctaagcgatg tggcgcttca
      241 aaaaacacca tagttctggt ttcattaacc aactgatcta aaaacttttc ccgttcgcca
      301 cttttacgcg gcaaaaaccc ttcgaaagta aatctgtcaa ccgacaatcc gctaaccgca
      361 agagccgtta atactgctga aggtcctgga ataacgctaa ctgcaactcc gctttcaatt
      421 gccgcctgca caatccgata acccggatca ctcacggttg gcatgccagc atctgaaata
      481 actaaaactt tatttccagc ttgaacgcgc tcaattaatt ctggtgactt actttcttca
      541 actgcgtcat aaaacgacca gatttgcgcg ttagtcgaaa tacctaaatc ttgaagtaac
      601 cgttttgctc gacgtgaatc ttcggcggca ataatgtccg cggtttcaag tgcttcacac
      661 aatcgtggtg aagcatctgc actgttgcca attggcgttg ccgccagaat caattgccca
      721 ctcacccgtc catcttcccc gatctaggtt ggtctaacaa ataagcggta actttagatg
      781 gtgagttcca cgcaattaga aacaacgcaa aaaccagcgc agtttgactt agatattaaa
      841 aaacctatgg ccggtggaat acgcggctgg tttggaccgc tggctgcaac tgtcgttgca
      901 gctgcgctac ggttaccaaa tttaggaact ccacacgctc tagttttcga tgaaacttac
      961 tacgccaaag atgccttaag tcttttaaat ttcggagttg aaaagaagtt tgttgatggt
     1021 gcaaacgaaa aattattggc atccgatggt caaaattatt tagatatttt tctaaatcaa
     1081 ccggcctacg tcgttcatcc accaatcggc aagtggataa tcgcaagtgg cgaagccatt
     1141 tttggcgcaa caccatttgg ctggcgtatt gcagtggcaa tcctaggaat tcttagcgtc
     1201 ttattaactg cgcgaataac ccgccgatta actaactcaa atttcatagg aactgtagcc
     1261 ggttttctac ttgccctcga tggattacac atcgcaatga gtcgcaccgc cttactcgat
     1321 acgccgctta gtttctttgt gctgtgtgca tttggcgcgc tgatacttga tcgcgaccga
     1381 aaaactaatg agtcatttag gtactggcgc tggataatga tcggctttct tggtttagcc
     1441 atcgcaacta aatggagtgg cctttacttt gcggcagcat ttggcttgct gatgttgatt
     1501 tgggatctca atcgccgaac aaaaatttcg cagtggctca aaaaagattt actgccggca
     1561 atacttatgc catttgtggc acttgcgatt tacatcgcta gttgggttgg ttggtttgcc
     1621 tcaagtgacg gttgggcaag aaattttgct gctgataaaa ataattcatt tttgccggaa
     1681 agcttggtca gcctatttca atatcacaaa gacatgtgga gttttcatac caacttaacc
     1741 actgcgcata actacgaagc caatccttgg ggctggccaa ttctttatcg cccaacaagt
     1801 ttcttttatg acacagccag catctgcggt ggaccaagtt
//
LOCUS       DBNHVN010000006         1880 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1412984, whole genome shotgun sequence.
ACCESSION   DBNHVN010000006 DBNHVN010000000
VERSION     DBNHVN010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1412984"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="AC16Y1_00095"
     CDS             complement(<1..1232)
                     /locus_tag="AC16Y1_00095"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR5830900.1"
                     /translation="MNHEKQVESQATQMHEFDSQATEIVFSYLRERLSLKETPLDFPG
                     DSSVLASAISGLINPNGNSVSKVMEIYDQVLSRSIISGDSPRFFAFIPVAPTKASLLF
                     DMVVSAASLQGISWLEAAGAVAAENQVLRWMTDSAGLPNTSGGTFVSGGSAANLAALT
                     VARDIGRRKLDSQVKRIPIRIAMSKQAHSSIGNSLSILDVEPLIVDTKTDQLTGEDLR
                     RALAMQDDSQPPVVAVVATAGTTNAGIVDELSSISKVAKEFDLWFHVDAAYGGAALLA
                     PSVKSLFSGIEFADSITMDPHKWWFAPFDVAAILYRDPKLAKSVHTQNASYLDVLHSE
                     EEVWNPTDYAYHLTRRARGLPLWFSVVVNGTNAYRDAVEASLKLTREVAQLIRNHPDL
                     ELVIEPTLSIILWRRKGW"
     gene            complement(1301..>1880)
                     /locus_tag="AC16Y1_00100"
     CDS             complement(1301..>1880)
                     /locus_tag="AC16Y1_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015658286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HIR5830901.1"
                     /translation="TNSSDAAIALPSEKAARLAVRTQQVLAYETDITNTVDPFAGSYA
                     VEAMTDEIESEILRLMEKIEQLGGAAAAVEIGYQKNAIEEVAYDYALQIEKGERIVVG
                     VNKFEISEEEKYEQLKVDLAIEQAQKAKLEKLRESRDDQSVQAALAELKNAAATTENV
                     LYPMKKALQLKATGGEVADALREIWGSYQPLK"
BASE COUNT          524 a          446 c          369 g          541 t
ORIGIN      
        1 tgccagcctt ttcgtcgcca caaaataatg gaaagtgtcg gttcaataac taactctaaa
       61 tctggatgat ttcgaattag ttgcgcaact tcgcgagtca actttaacga agcttcaact
      121 gcatctcggt acgcatttgt tccatttacg acaactgaaa accaaagcgg taatcctctg
      181 gctcggcggg ttaagtgata cgcatagtcc gttggattcc aaacttcttc ttcactatgc
      241 aaaacatcta agtaagaggc attctgagta tgcactgatt tagctaattt tggatctcga
      301 taaagtatcg ccgcaacatc aaaaggtgca aaccaccatt tatgaggatc catagttatc
      361 gaatctgcga actcaattcc agaaaatagc gatttcacac tcggtgcaag taatgcggct
      421 ccaccataag cggcatcaac atgaaaccaa aggtcaaatt cctttgctac tttgctaata
      481 ctcgaaagtt catcaacaat tcccgcattc gttgtacctg cagttgcgac taccgcaacc
      541 actggtggct gagaatcatc ttgcattgct aaagctcggc gcaaatcttc tccagttaac
      601 tggtcagttt tcgtatcgac tatgagtggc tctacatcca aaatagacaa cgaattacca
      661 atagatgaat gagcttgttt gctcatagca atccgtattg gaattcgctt aacctgcgaa
      721 tcaagtttgc gccgaccaat gtctctagcc acggtaagcg ccgccaagtt tgcagcagat
      781 ccccccgaaa caaaagttcc acctgaagta tttggcagtc cggcactatc tgtcatccaa
      841 cgtaaaactt gattctcagc agcaaccgca ccagcagcct ctaaccaaga aatgccttga
      901 agtgaagctg cggataccac catgtcgaaa agtaacgatg ccttggtagg cgccacaggg
      961 ataaaagcaa aaaatctcgg actatcaccg gaaataatcg aacgcgataa tacttgatcg
     1021 taaatttcca taactttaga aacagagttt ccattcggat ttatcaatcc tgaaattgcg
     1081 cttgccaaga cgcttgagtc gcctggaaaa tctaacggag tttctttaag tgaaagtctt
     1141 tcccttaaat aagagaaaac aatttcagtt gcttgagaat caaattcatg catttgagtt
     1201 gcctgtgact caacttgctt ctcgtggttc atatacccgc cggaaactag atatacgggc
     1261 cgccgacata attagccagc agggcaatcc gtaatcaaaa ttattttagc ggttggtaac
     1321 ttccccagat ttcacgtaag gcgtcggcca cttctccacc agttgcctta agttgtaggg
     1381 ctttcttcat tggataaagg acattttcag ttgtcgcagc ggcattcttt aactctgcta
     1441 atgcagcctg cacactctgg tcatccctgg attcacgtag tttttctaac tttgcttttt
     1501 gagcttgttc aattgctaag tcaactttta gttgctcata tttttcttct tcagaaattt
     1561 cgaacttatt cacaccaaca acgattcgtt cacctttttc aatttgcagc gcgtagtcgt
     1621 aagcaacttc ttcgattgca tttttctggt aaccgatttc aaccgcagca gcagcaccgc
     1681 caagttgttc aattttttcc ataaggcgta agatttctga ttcaatttcg tcagtcatgg
     1741 cttcaactgc atacgagcct gcaaatggat ccacggtatt agtgatgtca gtttcatatg
     1801 ccaaaacttg ttgggtgcga acagcaagtc tggcggcttt ttctgatggg agtgctatcg
     1861 ctgcatcaga tgaattcgta
//
LOCUS       DBNHVN010000007         1539 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1784708, whole genome shotgun sequence.
ACCESSION   DBNHVN010000007 DBNHVN010000000
VERSION     DBNHVN010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1784708"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="AC16Y1_00105"
     CDS             <1..406
                     /locus_tag="AC16Y1_00105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR5830902.1"
                     /translation="IPTDQHDGRSAYRDAIRALDNGQCVLIYPEGDVKVNSDASPRPW
                     RPGAVALAKSRLMPIVPIAHHDSRKLGSGSVSKSIVLALTKFIRRPKIHLNIGRPILP
                     EELTGKTPTEIGQLMFDRLVETWQQAVVAASR"
     gene            complement(396..>1539)
                     /locus_tag="AC16Y1_00110"
     CDS             complement(396..>1539)
                     /locus_tag="AC16Y1_00110"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017239954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR5830903.1"
                     /translation="RELAVQVAEDLTMASVNIGARVLSLYGGRAYEPQIEAITKGVEV
                     VVGTPGRLIDLIKQNHLDLSHIKVLVMDEADEMLDMGFLPDVEKLVSKCNPSRQTMLF
                     SATMPSQIIALAKRYMTSPTHIRAVDPNSESSLVDAIEQHVWRCHQMDKQELVARVLQ
                     AEGRGLTIIFCRTKRVASSLSDELASRGFAVGAVHGDLGQGAREQALRAFRSGKIDIL
                     VATDVAARGIDVADVTHVINYECPDDEKTYTHRVGRTGRAGAAGVAITFVDWQDINRW
                     QMINRALGLPFHDPIETYSSSDHVYTGLNIPKNISGSLPGSKQERAGLDAEKLEDLGE
                     TGARKGRKQPVKSSNTKTAAKKPEVKKEEKPRVQRERKRTRRATSD"
BASE COUNT          389 a          357 c          362 g          431 t
ORIGIN      
        1 tattccaacc gatcagcatg atggtcgaag tgcttatcga gatgcaattc gcgcattaga
       61 taacggacaa tgtgtactga tttatccaga aggtgatgtc aaagtaaata gtgatgcctc
      121 accgagacca tggcgaccgg gtgcggtagc gctagcaaaa tctcggttga tgcccattgt
      181 tccaattgca catcacgata gtcgcaaact tggttcaggt tcagtttcta aaagcattgt
      241 gctggcactg actaaattta ttcgtcggcc aaagattcac ctgaatatcg gacggccgat
      301 cttgcctgag gaattaactg ggaaaacgcc aacagaaatt ggtcaactta tgtttgatcg
      361 attagttgaa acttggcaac aagctgtagt tgcggctagt cgctagtagc acgccgagtg
      421 cgtttacgtt cgcgctgcac gcgaggtttt tcttcttttt tgacttcagg cttcttggcc
      481 gcagttttcg tattgctact ttttacaggt tgtttcctgc ctttacgagc gccagtttca
      541 cctaggtctt caagtttttc agcatcaagt ccagctcgtt cttgtttact gcctggtaga
      601 gatccagaaa tattttttgg aatatttaaa ccggtataaa cgtgatcact tgatgagtaa
      661 gtttcgatcg gatcatgaaa cggtaagcca agtgcgcggt taatcatttg ccaacgattt
      721 atgtcttgcc agtcaacaaa agttattgcc acacctgcag caccagcgcg accagttcga
      781 cctacgcggt gggtgtaagt cttttcgtcg tctggacatt cgtaattaat tacatgggta
      841 acatcggcta cgtcgatacc gcgtgcggca acatctgtcg ctaccaagat gtcgattttt
      901 cctgaacgaa atgcacgaag tgcctgttca cgagcgcctt gtcctaagtc accatgcaca
      961 gcaccgacgg caaaaccacg gctagcaagt tcgtcactta atgagctggc aacacgtttg
     1021 gtgcggcaga aaataatggt gagtccgcga ccttcggctt ggagtacgcg agcaaccagc
     1081 tcttgtttat ccatttgatg acagcgccag acgtgctgtt cgattgcatc aaccagtgaa
     1141 gattcactat ttggatcaac agcgcgaata tgtgttggcg aagtcatata gcgcttggct
     1201 aaggcaatta tttggctggg catagttgcc gagaacaaca tagtttggcg acttggatta
     1261 catttgctaa ctaatttttc aacatcgggc aagaaaccca tgtcgagcat ttcatcggct
     1321 tcgtccatca ctaagacttt gatgtgcgat aaatctaaat ggttctgttt aattaagtcg
     1381 attaggcgtc cgggtgtacc gactaccacc tcaacacctt tggtaattgc ctcaatttgc
     1441 ggctcgtagg cacggccacc ataaagactc agaacgcgag caccaatatt tacgctggcc
     1501 atagttaggt cttcggcaac ctggaccgct aattcacgg
//
LOCUS       DBNHVN010000008         4338 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_893168, whole genome shotgun sequence.
ACCESSION   DBNHVN010000008 DBNHVN010000000
VERSION     DBNHVN010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_893168"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..270
                     /locus_tag="AC16Y1_00115"
     CDS             55..270
                     /locus_tag="AC16Y1_00115"
                     /inference="COORDINATES: protein motif:HMM:NF025192.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004658 - propionyl-CoA carboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit epsilon"
                     /protein_id="HIR5830904.1"
                     /translation="MTHTSDAEQFKVVRGEPTAEELAAIIVVLRNQAAVEAKKQSVKP
                     EWNAAQRTIRKQFAHGTNMWRRSALPQ"
     gene            275..895
                     /locus_tag="AC16Y1_00120"
     CDS             275..895
                     /locus_tag="AC16Y1_00120"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014144534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR5830905.1"
                     /translation="MKRQVLLASASASRYRLLVEGGITPLVQVSAVDEDAIAAQLGNI
                     SPAGLVEKLAIAKGQAVASDITVTENRNLIIIAADSMLEFDGQMYGKPLTAANAKLRW
                     QAMRGQSGYLHSGHWIRDLATGQEITGVAGAHIHFTDVTDAEIDKYVASGEPLNVAGA
                     FTHEGRSAAFINSIDGDVAAVAGMSMRLIRELTAKMGISWTEMWDN"
     gene            980..2737
                     /locus_tag="AC16Y1_00125"
     CDS             980..2737
                     /locus_tag="AC16Y1_00125"
                     /EC_number="6.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018510407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HIR5830906.1"
                     /translation="MKSVLIANRGEIAIRVARACKDAGYKSIAIYAEPDRDALHVKAA
                     DEAFALGGQTAAESYLVIDKIIAAAKKSGANAIHPGYGFLSENAEFAQAVIDAGLIWI
                     GPPPAAIRDLGDKVSARHIAAKAGAPQVKGTADPVKDSSEVVAFAKENGLPIAIKAAF
                     GGGGRGLKVARTLEEIPELYESAVREAVAAFGRGECFVERYLDNPRHVETQCLADEHG
                     NVVVVSTRDCSLQRRHQKLVEEAPAPFLTDAQRDELYASSKAIMKEAKYVGAGTCEFL
                     IGADGTISFLEVNTRLQVEHPVSEEVTGLDLVREQFRIAQGEKLGYSDPEVRGHSIEF
                     RINGEDPGRGFLPAPGSLTVWEPPSGPGIRLDAGFMQGDVIGGNFDSLLAKLIVTGRD
                     RKEAIERSRRALAEFQINGIATALTFHQVVVNDPAFAPIDPTESFKVHTRWIETEFDN
                     QIPPYSGDQGEMNQSAQRAPITVEVNGRRVEVVLPADFAISKSANKSAGGPPKRAGKK
                     AAGGDGGNSVTSPMQGTIVKVAVSDGQSVNQGDLVIVLEAMKMEQPLTAHKSGTVTNL
                     TAQIGTTVPSGTVLLEITD"
     gene            complement(2734..4104)
                     /locus_tag="AC16Y1_00130"
     CDS             complement(2734..4104)
                     /locus_tag="AC16Y1_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009717285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone dehydrogenase"
                     /protein_id="HIR5830907.1"
                     /translation="MPKVVIIGGGPGGYEAALVATQLGGDVTLIERDGVGGAAVLTDV
                     VPSKALVAVAEAAIDAREANVIGVQTSEVTIEFQKVNSRLKQWATNQSRHIFDSLIAA
                     GVQVISGSGELLSSNQVKVTSASGEQILDADVILLSTGARPRELPTAHPDGERILNWK
                     QLYDLPELPEKLIVVGSGVTGAEFAGAYQALGSNVVLVSSREQVLPGEDSDAAAVIEQ
                     VFSDRGMTVLSQSRAKAVRRTSTGVEVELESGEVVDGTHCLIAVGAIPNTETLNLAAA
                     GVDKTETGFITVDKVSRTNIRGVYAAGDCTGVFMLASVAAMQGRIAMRHALGDAVNPL
                     NLETVSSNVFTDPEIATVGISQREAESQADSIEAVILPLATNARAKMQGITEGFVKLY
                     CKTDSGIVVGGVVVAPKASELIFAIALAVENRLTVEQVSHTITVYPSLSGSIAEAARR
                     LRFRNS"
BASE COUNT         1207 a          986 c         1008 g         1137 t
ORIGIN      
        1 gttaaaaggt aaacgtcaga cacttccacc taagaaacac ggcaacattc cactatgacg
       61 cacacatctg acgccgagca attcaaagtg gttcgtggtg aaccaactgc agaagaatta
      121 gccgcaataa ttgtggtact gcgtaatcag gctgctgtag aagcgaaaaa acaaagtgtc
      181 aaacccgaat ggaatgctgc gcagagaaca attcgcaaac agtttgctca tggaaccaat
      241 atgtggcgca gatcagccct tccccaataa ttagatgaaa cgccaagtac tacttgcatc
      301 agcatcggca tcgagatatc gactacttgt tgagggtgga attacaccac tcgttcaagt
      361 aagtgctgtc gatgaagatg cgattgcagc ccaacttgga aatatttcac cggcaggttt
      421 agttgaaaaa ctcgcaattg ccaaagggca ggcagtcgct agtgatataa ctgtcaccga
      481 aaatcgaaac ttgattatta ttgcagccga ttcgatgctg gaatttgacg ggcaaatgta
      541 tggcaaaccg cttacagcag ctaacgcaaa attacgctgg caggcaatgc gcggtcagtc
      601 tggatacctg cacagtggtc attggatcag ggatttagcg accggtcaag aaattaccgg
      661 agttgctggt gcgcatatac attttacaga tgtcaccgat gccgaaattg ataagtatgt
      721 tgcctcaggt gaaccactta atgttgcggg tgctttcaca cacgaaggta gaagtgcagc
      781 cttcataaat tcaatcgacg gagatgtcgc tgcggttgcc gggatgtcta tgcgtttgat
      841 ccgagaatta acggcaaaga tgggtatttc ttggactgaa atgtgggaca actaaaccac
      901 atatttatcg ttgggttgcg aaacaataga cttgccagac aaggtaattc gacaggggta
      961 ataggtgagt aaccgaaaaa ttaaatcggt actgattgcc aatcgcggcg aaattgcaat
     1021 aagagttgcc cgtgcttgta aagatgccgg atacaaaagt attgccatat atgccgagcc
     1081 tgaccgagat gctttacacg taaaagcagc ggatgaagcc ttcgcattag gtggccagac
     1141 tgcagccgag agctacttgg tgatcgacaa aattattgct gctgcaaaaa agtctggggc
     1201 aaatgcaatc catcctggct atggattttt atctgagaat gccgagtttg cccaggctgt
     1261 aattgatgcg ggacttatct ggattggtcc gccgcccgcc gcaattcgtg accttggcga
     1321 caaagtttca gcaagacata tcgccgcaaa agctggtgct ccgcaagtca aaggtacagc
     1381 tgacccagtc aaggacagct cagaagtagt tgcttttgcc aaagaaaacg gattgccaat
     1441 cgcaatcaaa gccgcatttg gtggtggtgg acggggctta aaagtagctc ggacacttga
     1501 agaaattcca gaactttatg aatctgccgt tcgtgaggct gttgcggctt ttggtcgcgg
     1561 tgaatgtttt gtcgaacgct atctagacaa tcctcgccac gttgaaactc agtgtctggc
     1621 agatgagcac ggaaatgttg ttgttgtctc aactcgtgat tgttcgctac agcgacgcca
     1681 tcaaaaatta gttgaagaag caccggcacc tttcctaact gatgcccaac gcgatgagct
     1741 ttacgcttcc agtaaagcaa tcatgaaaga agctaaatat gttggtgccg gtacttgtga
     1801 atttttaatt ggtgcagatg gaactatttc tttccttgaa gtaaacactc gtttacaagt
     1861 tgagcatcca gttagtgagg aagttactgg tcttgatttg gttcgtgagc agttcagaat
     1921 cgctcaaggt gaaaagcttg gttattcgga tccagaagtc cgtgggcact caattgaatt
     1981 tcgaatcaat ggtgaagatc caggccgtgg ctttttgcca gcgccgggat cacttaccgt
     2041 ctgggaaccg ccaagtggtc cgggaatccg attagatgct ggattcatgc agggcgatgt
     2101 aattggtgga aactttgact cgctacttgc caaattaatt gttactggac gcgatcgaaa
     2161 agaagcaatt gagagatccc gaagggcgct tgcggaattt caaataaacg gaatcgcaac
     2221 tgcgctaact tttcatcaag tcgtggtgaa tgacccagct tttgcaccaa tcgatccaac
     2281 ggaatcgttt aaggtgcaca ctcgatggat tgaaacagaa tttgataacc aaattcctcc
     2341 atattctggt gatcaaggtg agatgaatca gtctgcacaa cgggcaccaa ttaccgttga
     2401 agtaaacggt cgtcgagttg aagttgtact cccagcagat tttgccattt caaaatcagc
     2461 aaataaatct gctggtggtc caccgaagcg agccggcaag aaagccgctg gtggtgatgg
     2521 tggtaattcg gtaacctcac caatgcaagg aacaattgta aaagttgcgg taagtgacgg
     2581 tcagtcagtt aatcaaggtg acttagtaat tgttttagag gcaatgaaaa tggaacaacc
     2641 gttgactgca cataaatcag gtacggtaac taatttaacc gcgcaaatag gcaccacagt
     2701 gccaagtggc acagtgctac ttgagattac tgactaacta ttcctaaatc gcagtcggcg
     2761 cgcagcttct gcaattgatc cgcttaatga cggataaacc gtaattgtgt gtgaaacttg
     2821 ctcgacagtt agtcgatttt caaccgctag ggcaatggca aaaattaact cacttgcttt
     2881 tggcgccact acaacgccgc cgacgacaat tccagaatca gttttacagt aaagcttcac
     2941 gaaaccttcg gtgatacctt gcatctttgc gcgagcatta gtagccaacg gaagtatgac
     3001 tgcttcaatg gaatctgctt ggctttcagc ttcgcgctga gaaattccca ccgtggcaat
     3061 ttctggatca gtaaatacat tgcttgaaac agtttctaaa tttaatgggt tgactgcatc
     3121 acccaaagca tgtctcattg ctatccgacc ttgcattgca gccactgaag caagcataaa
     3181 gacaccagtg caatcaccgg ctgcataaac tcctcgaatg tttgttcgcg aaactttgtc
     3241 tacggtaata aaaccagttt cagttttatc aacaccagca gccgccaaat taagtgtctc
     3301 tgtgtttgga attgcaccaa ctgcgattag gcagtgtgtg ccatctacta cttctccaga
     3361 ttccagttca acctcaactc cagtcgaggt gcgccgcaca gctttggccc gagattgact
     3421 tagcactgtc atgccacgat cgctgaaaac ttgctcaatt actgcagcgg catctgagtc
     3481 ttcaccaggc aaaacctgtt ctcgcgacga aactaaaact acgtttgaac caagcgcttg
     3541 gtatgcacca gcgaattcag caccggtaac cccagatcca acaacaatta acttttccgg
     3601 taattctggt aaatcataaa gttgtttcca gttgagaatt cgctcaccat ctggatgtgc
     3661 agttggtaac tctcgcggcc gagctccagt agaaagtaaa attacgtcag catcaagtat
     3721 ttgttcgccg ctggcactgg taacttttac ttgattgcta cttaataatt caccagatcc
     3781 agaaattact tgaacgccgg ctgcaattag tgaatcaaaa atatgccggc tctgattcgt
     3841 agcccactgc tttagcctgg aatttacttt ttgaaactcg attgtgactt cagatgtttg
     3901 tacaccaatc acgtttgctt ctctggcatc aatggctgct tcggcaactg caactagtgc
     3961 tttactcggt acgacatctg ttaatacagc ggcgccgcca acaccatcgc gttcgattaa
     4021 agtgacgtcg ccaccaagtt gagttgcaac tagcgctgct tcgtaaccac cagggccacc
     4081 gccaataatc actactttgg gcacgtgtct atatttcctt acttcgtagc aatttactta
     4141 cttaccttgc ttgtttcttt attaaatttg tgcaaaaact tcattaacca tttgcacagt
     4201 tgagttcggg tttataaagg caaacctgcc aacggtttca cctttccagg tcgttggcgt
     4261 cacaaatgca atttggcttt cgagtagtcg atcttgcaac ttggcatagt cttgggcctg
     4321 ccagcctttt cgtcgcca
//
LOCUS       DBNHVN010000009         3971 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_2008113, whole genome shotgun sequence.
ACCESSION   DBNHVN010000009 DBNHVN010000000
VERSION     DBNHVN010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_2008113"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="AC16Y1_00135"
     CDS             complement(<1..723)
                     /locus_tag="AC16Y1_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013415736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D-related
                     protein"
                     /protein_id="HIR5830908.1"
                     /translation="MTDTYAGSYETTSGRVYTLQGQDWDTVQGAAEGENERIVVNMGP
                     QHPSTHGVLRLILELEGETVTEARCGIGYLHTGIEKNLEYRSWTQGVTYVTRMDYLSP
                     FFNETAYCLGVEKLLGITDQIPERATVIRVMMMELNRISSHLVALATGGMEMGALTAM
                     IFGFRERESVLDIFELVSGLRMNSAYIRPGGVAQDLPDGAEEKIRELIKALPNRFKDT
                     ADMLVDNSIWLGRTQGIGILDLA"
     gene            complement(723..1433)
                     /locus_tag="AC16Y1_00140"
     CDS             complement(723..1433)
                     /locus_tag="AC16Y1_00140"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012393325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR5830909.1"
                     /translation="MSDQEQTNNLVPTGQKLLEVTTVRKGMFGVDGAGDTSGFGGLVS
                     VVPVPQDSPRPYGGYFDVIADELLASLKDFGTTWEKAIEKVVIAHGEMTIYVAASELP
                     AVAKTLRDEPGLRFELCLGVSGVHYPEHAGKELHAVYHFRSITHNRTLRVEVTAPDSN
                     PHVPSIISIYPTNDWHEREAYDFFGIIFDGHPHLTRIQMPDDWQGHPQRKDYPLGGIP
                     VEYKGATIPPPDQRRSYS"
     gene            complement(1430..1984)
                     /locus_tag="AC16Y1_00145"
     CDS             complement(1430..1984)
                     /locus_tag="AC16Y1_00145"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NuoB/complex I 20 kDa subunit family protein"
                     /protein_id="HIR5830910.1"
                     /translation="MGLEEKLPSGVLLSTVEGLAGYARKGSLWPATFGLACCAIEMMA
                     SGAGRYDLARFGMEVFRASPRQADLMIVAGRVSQKMAPILRQIYDQMPNPKWVLSMGV
                     CASSGGMFNNYAIVQGVDHVVPVDIYLPGCPPRPEMLIDAILKLHDQISDTKLGANRE
                     REIVELERAALVAPHTSELKGLLR"
     gene            complement(1994..2362)
                     /locus_tag="AC16Y1_00150"
     CDS             complement(1994..2362)
                     /locus_tag="AC16Y1_00150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR5830911.1"
                     /translation="MGSGNVYLPILFLGLLAFGFAVFSVAAASFTGPKRYNRAKVDAY
                     ECGINPAPQSIGGGKFPIKYYLTAMLFIIFDIEIVFLYPWAVHFDALGLFGLIEMVIF
                     ILTVLTAYAFVWRRGGLEWD"
     gene            complement(2566..3255)
                     /locus_tag="AC16Y1_00155"
     CDS             complement(2566..3255)
                     /locus_tag="AC16Y1_00155"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018528245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HIR5830912.1"
                     /translation="MSSADLNKQPQEVAAMFDGVAEKYDLTNDILSLGQTRLWRRAVV
                     NAVKPEHGEKILDLAAGTGTSTQPFYLAGADPVACDFSAGMIEVGKRKFPHLNFVQGD
                     ALDLPFADNTFDAVTISFGLRNVQDTKKALAQMYRVTKPGGRMVICEFSHPTWKPFRT
                     TYLEYLMKALPAIAKKVSSNPDAYIYLAESIRAWPNQELLAAMIKDSGWKNVEWLDLT
                     GGIVTLHRATK"
     gene            3332..>3971
                     /locus_tag="AC16Y1_00160"
     CDS             3332..>3971
                     /locus_tag="AC16Y1_00160"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HIR5830913.1"
                     /translation="MSQPASTNIAKQVYVATQKIADIPNLLNLIPQYGMAWVSGPADN
                     HEGLVAVGEVDRYEVSGPERFSRAQSWWRNWAATAVGDEPIAFTSFTFANDSGTSIVV
                     VPEVIVSRKNGVTTLTITSKTPISKNQINDEIAQLTKPRSIVDQKLQSQFLAGSNPVE
                     LWSKAVDTAVARITHGELDKVVLARDLIAQLDQPLNVGSLLRKLNQTFPECWT"
BASE COUNT         1109 a          913 c          829 g         1120 t
ORIGIN      
        1 agctaaatcg agaattccaa ttccctgggt gcgaccaagc caaattgaat tatctaccaa
       61 catgtcggca gtatctttaa agcgatttgg tagcgctttg attagctctc taattttttc
      121 ttcggcgcca tcaggtaaat cttgcgcgac accacccggc cggatgtaag cgctattcat
      181 gcgcagtccg cttactaatt cgaaaatgtc gagaactgat tcacgttcgc ggaaaccgaa
      241 aatcattgca gttaatgcgc ccatttccat cccgccggtt gcaagagcaa ccaggtgtga
      301 cgaaatacgg ttcagttcca tcatcatcac gcgaattaca gtggcacgtt ctggaatttg
      361 atctgtaatg ccaagaagtt tttctacgcc aagacagtaa gcagtttcgt taaaaaatgg
      421 cgacaagtaa tccatgcgcg ttacgtaagt aacaccttga gtccaagagc ggtactctaa
      481 attcttttca attccagtat gaagatagcc aatgccgcaa cgtgcttcag ttacggtttc
      541 gccctccaat tcaagaatga gtcgaagtac accgtgagtt gaagggtgtt gtggacccat
      601 gttgaccaca atgcgttcat tttcaccttc agctgcacct tgtacggtgt cccaatcttg
      661 cccttgaagt gtgtaaacgc gacccgaagt tgtttcgtat gaaccagcgt atgtatctgt
      721 cattagctgt acgacctccg ctgatcaggt ggtggaatag tcgcgccttt gtactcaact
      781 ggaattccac caagtggata atctttacgt tgcggatgac cttgccaatc atctggcatt
      841 tgaattcggg ttaaatgtgg gtgaccatca aaaattattc caaaaaaatc ataggcttcg
      901 cgttcgtgcc aatcgttagt tggataaatc gaaattattg aaggtacatg cggatttgaa
      961 tcgggcgcag ttacctcaac tcgaagtgtg cgattatgcg tgattgatct gaaatggtaa
     1021 accgcatgta attcttttcc agcatgttct ggatagtgca cgccactaac gcctaaacaa
     1081 agttcgaacc gtaaacctgg ttcgtcgcga agtgttttcg ccacagctgg taattcactt
     1141 gccgcaacat aaatcgtcat ttcgccatga gcaattacaa ctttctcaat tgctttttcc
     1201 caggtggtac caaaatcttt aagtgatgcc aataattcgt cagcaatgac atcaaagtag
     1261 ccaccatatg gccgtggtga atcttgcgga actggaacaa cagaaacaag tccgccaaaa
     1321 cccgaagtat cccctgcgcc atcaactccg aacattcctt ttcgcacagt cgtcacttca
     1381 agtaactttt ggccagttgg aactaaatta ttagtttgct cttgatcact catcgaagaa
     1441 ggcctttgag ttctgaggta tgtggtgcta ccaacgcagc acgttctagt tcaacaattt
     1501 cacgttcgcg atttgcacca agtttggtgt cagaaatctg atcatgtaat ttcaaaatcg
     1561 catcgatcaa catttcggga cgtggtggac atcctggtaa gtaaatatca actggcacaa
     1621 cgtgatctac accctgcaca attgcatagt tgttgaacat accgcctgag gatgcacaaa
     1681 cacccattga taaaacccat tttggatttg gcatttggtc gtaaatttgg cgaaggatcg
     1741 gtgccatttt ttggctaact cgtccagcca caatcattaa gtctgcttga cgaggtgagg
     1801 cgcgaaaaac ttccattcca aagcgggcta aatcatatcg accggcacct gatgccatca
     1861 tttcaatcgc acagcaagct agaccaaatg ttgccggcca cagtgaacct ttgcgagcgt
     1921 agccagccaa cccttcgact gttgaaagta aaactccact aggtaatttc tcttctaaac
     1981 ccatgtcgcc tctttaatcc cactcaagtc caccacggcg ccaaacaaag gcatacgcag
     2041 ttaagacagt cagtatgaaa ataaccattt caattaagcc aaataaacca agtgcatcaa
     2101 agtgaactgc ccatggatat aagaaaacaa tttcgatgtc gaaaataata aaaagcatgg
     2161 cggttaagta gtacttaatc ggaaactttc ccccgccaat actttgtggt gctggattga
     2221 taccgcattc gtaggcgtct acttttgcgc ggttatacct ttttggtcca gtgaatgaag
     2281 ctgccgcaac tgaaaaaact gcgaatccaa atgcaagtaa accaaggaac aatatgggaa
     2341 ggtaaacatt tcctgatccc acagttgcca cctttcacga ccatttttaa cgttctgcaa
     2401 tgtcggctag ttaatcctag atccgataaa gcctttaaaa atcccgactt tgtgcttttg
     2461 tgaatgaatt cactagcact gtttaagtct attaccgaag taagttgggc aattccgcag
     2521 tctgacaaag accggcttac ttcgtcgccc tgaacttatg cgggcttact tcgtcgcccg
     2581 gtgcaacgtc acaataccgc cagttaaatc aagccactca acatttttcc aaccgctgtc
     2641 tttaatcatg gcggcaagta gttcctgatt tggccaagct ctaattgatt cggctagata
     2701 gatgtacgcg tcaggattac ttgaaacttt tttggctatt gcaggtagag ctttcattaa
     2761 atactcgagg tacgtggttc taaatggttt ccaagttgga tgactaaact cacaaatcac
     2821 cattcggcca cctggttttg taactcggta catttgtgca agtgcttttt tagtatcttg
     2881 aacgtttcgt aaaccaaaag aaatggttac cgcatcaaaa gtattgtcgg caaaaggtaa
     2941 gtcgagtgca tcgccctgca caaaatttaa gtgtggaaac ttccgttttc caacttcgat
     3001 cattccggct gaaaaatcgc aagcaactgg atcggcgcct gctaaataaa aaggttgagt
     3061 acttgttcct gtaccagctg ctaaatctaa aattttttcg ccgtgttccg gttttactgc
     3121 attaactact gcgcgacgcc ataatcttgt ttgacctaaa gacaaaatgt catttgttaa
     3181 atcatatttt tcagcgacgc catcaaacat tgccgctact tcttgtggct gcttatttaa
     3241 atcggcactg gacatagtta tagtctgccc actttgagcg cattcgtcat atttagcaag
     3301 tctcatcggt taggcgtagg cttaatttgt tatgagtcag ccagcgagta caaatattgc
     3361 taaacaggtt tacgtcgcta cccaaaagat tgctgatatt ccaaatttac ttaatttgat
     3421 tccgcaatat ggaatggctt gggttagtgg tccagctgac aatcacgagg gtttagttgc
     3481 agtgggcgaa gttgaccgct acgaagtatc tggtcctgaa cggtttagtc gggcgcaaag
     3541 ctggtggcga aattgggcgg ccactgcggt cggtgatgag ccaatcgcat ttacctcttt
     3601 tacttttgcc aatgattcgg gaacttccat tgttgtagtg cctgaagtta tcgtttctcg
     3661 caaaaatggc gtaaccaccc taactattac tagcaaaact ccgatttcaa aaaatcaaat
     3721 aaacgatgag attgctcaat taactaagcc gcgatcaata gtcgaccaaa aattgcagtc
     3781 acaatttctg gctggatcga atccagttga actgtggagt aaggctgtag acactgccgt
     3841 tgcccgaata acacatggtg agttagacaa agttgtacta gctcgagatt taattgctca
     3901 attagatcag cctttaaatg tcggctctct acttcgtaaa ctaaatcaaa cttttccaga
     3961 gtgttggact t
//
LOCUS       DBNHVN010000010         4842 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_299501, whole genome shotgun sequence.
ACCESSION   DBNHVN010000010 DBNHVN010000000
VERSION     DBNHVN010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_299501"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1995
                     /locus_tag="AC16Y1_00165"
     CDS             <1..1995
                     /locus_tag="AC16Y1_00165"
                     /inference="COORDINATES: protein motif:HMM:NF020075.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR5830914.1"
                     /translation="TSFTVAGSGHGHGVGLSQYGAYGMALSGTPADQILTYYYAGTAI
                     TNYDYTTVNNANIRVNVEGDVAFMYLRFEDDPSNTNAVATPPAITISGTPVANVAFNT
                     IYTFALTGTSFSLSGSGIQTVTGTSAQITWDNASTLANVNGGPAGSDGAGLLGNCSTS
                     TCPNRYKFGAIDIFVSQLKPNVVVTQDLQAVVTLKLNTEYLYGIGEVPSSWHSEVLKA
                     QAIASRTYASRKLQDSPNADRDDCGCHIFSTTVDQAYVGFSKQYLTDGNRWVDAVNAT
                     VDPANPNIGQVITSSGNLIIAYYSASTGGKTQPLNEWAGGSASYLTSVVDTLALDDRV
                     RNPLRTWSVTVDRNTLTTRMQAQGITVSDIANVAITGSYSSGGVSQLTLTNSSGGTTV
                     VNVGPGQRVTPDELRTMFGAFSTYFTAITANGVVQSTPTPTPTATPTPTPTPVPSTET
                     PAPAPATPAAPAAPAPVAAAPASASKPVRSITSVSWPSSKIKPGATSVAGRVSPAQAG
                     VAVSFQVLRNGGWTTVATDVTGRQGAWRVNWASVTAGTHKVRVVAATKVNTVATGTRT
                     LSAVGNVTLVGPTRSNRNSQIALRGTVTPSQSGVPVQVQRKIGSGAWKTVGTASTDGA
                     GNWAFTVNSGAAKSTSRFRVVISDGRVGSITSKTLATSIR"
     gene            2061..4229
                     /locus_tag="AC16Y1_00170"
     CDS             2061..4229
                     /locus_tag="AC16Y1_00170"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR5830915.1"
                     /translation="MFSRIGLLVVRFPKVILAIYLIFIAASAAVGFGVFNNVKSQGYD
                     NPSSDSAKVDTLLRDDFKVRENSVVFIVDTPTAVNDPATEKVVTEIANEVAQQEYVDR
                     VATYWSTGMQASMVSVDGKATVISIYFDEGIDPETATKTAGELQAKFDGPRGDTQIYV
                     AGIQTVFFAINDQIKSDLVKAESIAIPLNIVMLLIIFGTAVAAGLPMIVALGSILGSF
                     LTLFAISQFTDISVFALNLITGLGLGLGIDYALLIVNRFREEIHAGRDVAQSVTKTIA
                     TAGRTVFFSGVAVMLVLASMILFPQYFLKSFAYAGISVVFFAIMASLFALPAVLALLG
                     HRVDKWRIRKSALITKEDGAWSVIARTVMRRPLVFILASVALLSVFAYPAKDVKFGQV
                     DERVLPPTSPVVVAADVSRTRFDGQAATPIELVIPKASASESQVETLAKQLADLDGVV
                     SVLTPKSFTKDGLTAPLPMALPEQSTTDLYRISVVQEQSPRNEASVEMVRQIRDFDLP
                     QGTLVGGASAIYVDSQAGISDYLPKVITWLMIATLLVLFMYTGSILLPIKAVLLNVAS
                     LAATLGVITYIFEEGNLMWLVGDFTVTGTVDTSTLVLVAVVAFGLSMDYELFMLSRIK
                     EEHDNGADTEDAVSFGLQRSGRIISAAALLIALVFITFVTSGVTSIKMLGLGTAFAIL
                     LDATLIRAILVPAFMRIAGKWNWWAPKPLAKFHARFGISD"
     gene            4236..4535
                     /locus_tag="AC16Y1_00175"
     CDS             4236..4535
                     /locus_tag="AC16Y1_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014738848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4031 domain-containing protein"
                     /protein_id="HIR5830916.1"
                     /translation="MAILIDTAIWPWRDWLWCHMVSDTSVEELKEFASKLGIPERGFQ
                     GDHFDVPEHMREIAISHGAKVVTSREILQALYKSGIRKRPSERHGRPKNVPIHQP"
     gene            4571..>4842
                     /locus_tag="AC16Y1_00180"
     CDS             4571..>4842
                     /locus_tag="AC16Y1_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR5830917.1"
                     /translation="MSNTVPNAPVNLLKFAYLSIATGVVVFSLKLVAWWFTGSVGLLS
                     DALESTVNIVAAVIALLALRTAMKPADQKHHFGRGKAEYFSASVEG"
BASE COUNT         1284 a         1085 c         1116 g         1357 t
ORIGIN      
        1 acatcattta ccgttgctgg atctggtcat ggtcacggtg ttggcttatc tcagtacggt
       61 gcttatggca tggcactcag cggcaccccg gccgatcaga tacttaccta ctactacgcc
      121 ggtacggcaa tcacgaacta cgactacacc accgttaaca acgcaaacat tcgagtgaac
      181 gttgaaggcg atgtcgcatt catgtatttg cgctttgaag acgatccgtc aaataccaac
      241 gccgtagcta cgccaccagc catcacaatt agtggcacac cggttgcaaa tgttgctttt
      301 aacacgatct acacgtttgc cctaactgga acttctttta gtttgagtgg aagcggaatt
      361 caaacagtaa ccggaactag cgcacagata acttgggata acgcctcaac acttgcaaac
      421 gtaaatggtg gtccagctgg aagtgatggc gctgggttac ttggaaattg ttcaactagc
      481 acctgtccta atcgctacaa attcggcgcc atcgatattt ttgttagcca actgaaacca
      541 aatgttgttg taacacaaga tttgcaagca gtcgttacgt taaaactaaa taccgaatac
      601 ctttatggaa tcggcgaagt gccgtctagt tggcatagcg aagtattaaa agcgcaagca
      661 attgcctcgc gtacttatgc atcgcgtaaa ttacaagatt caccaaacgc tgatcgcgac
      721 gactgcggtt gtcacatttt ttcaaccacc gtagatcagg cttacgttgg atttagtaaa
      781 cagtacttaa cagatggaaa tcgctgggtt gatgcggtca atgccacagt cgatccagct
      841 aatccgaaca tcggacaagt tatcaccagc agtggcaact taatcattgc ttactactct
      901 gcatcaaccg gcggaaaaac ccagccctta aatgaatggg ccggcggaag tgccagttac
      961 ttaacctctg tcgttgacac actggcactt gatgaccggg taagaaatcc actacgcacc
     1021 tggtcagtaa cagttgatcg caatacttta actacgcgca tgcaagcaca aggtattacg
     1081 gtttcagata tcgcaaatgt tgcaattact ggcagttact caagtggcgg cgtaagtcag
     1141 ttgactttaa ccaactcaag cggtggcaca accgtagtca atgttggtcc tggtcagcgg
     1201 gtaacacctg atgaattgcg cacaatgttt ggggcttttt caacctactt caccgcgatc
     1261 acggcaaacg gagtggttca aagtacgcca actccaacac caactgctac cccgactcca
     1321 actccaactc ctgttccatc aactgaaacg cctgcgccgg cgccggcaac tccagcagca
     1381 ccagctgcgc ctgcgccagt ggctgcggct cctgcatcag caagtaagcc ggtgcgttca
     1441 attacttcgg ttagctggcc aagttcaaaa attaaaccag gtgcgacctc ggtagctggt
     1501 cgagtaagtc ctgcccaagc cggagttgct gtaagtttcc aagttttacg caatggtggc
     1561 tggaccacag ttgctactga tgtaactggc agacaaggtg cttggcgagt taattgggca
     1621 tcagtaacag ccggcacaca caaagttcga gttgttgcag ccacaaaagt aaatactgtt
     1681 gcaactggta ctcgaacatt aagtgcagtt ggcaacgtga ctttagttgg tcctactcga
     1741 agtaatcgaa attctcagat cgctttgcgt ggaacagtaa ctccgagcca aagcggtgtg
     1801 cccgttcaag tgcagcgcaa aatcggaagt ggcgcttgga aaactgtagg taccgcttca
     1861 acagatggcg ccggaaattg ggcattcacc gtcaattcgg gcgcagctaa atcgacatct
     1921 cgcttccgtg ttgtgatctc cgatggacgt gtaggcagta tcacttcaaa aacactggca
     1981 acttcaattc gctaagccca acgccaaagc gagtcttttt aatccatgaa agactgtaga
     2041 tcttagttac aggagtttta gtgtttagcc gaattggcct attagtagtc cgttttccaa
     2101 aagtaatctt ggcaatttat ttaattttta ttgctgcctc agccgccgtc gggtttggtg
     2161 tttttaacaa tgtgaagtca cagggttatg acaatccaag cagtgattca gctaaagtcg
     2221 acacgctttt acgtgatgac tttaaagttc gggaaaacag tgttgtattt atcgttgata
     2281 cgccaactgc agtaaatgat ccagcaaccg aaaaagtcgt tacagaaatt gcaaatgaag
     2341 tagctcagca agagtatgta gatcgagttg caacttattg gtcgaccggc atgcaagcct
     2401 caatggtttc tgtcgatggt aaagccaccg taatcagtat ttactttgat gaaggaatcg
     2461 atccggaaac tgctactaaa acagccggtg agctacaggc aaaatttgat gggcctcgtg
     2521 gtgacacaca aatttatgtc gcgggtattc aaacagtttt cttcgcaata aatgatcaaa
     2581 tcaaaagtga cttagttaaa gcagaatcaa tcgcaattcc gctaaatatt gtgatgctgt
     2641 taattatttt tggtacggca gttgcagctg gcttgccgat gattgttgcc ttaggttcaa
     2701 tcttgggatc atttttaaca ttatttgcaa ttagccaatt cactgacatt tcagtttttg
     2761 cactcaatct aattaccggt cttggtcttg gtcttggcat tgattacgcg cttttaatag
     2821 ttaatcggtt ccgcgaagaa atacatgctg gccgtgacgt cgcacaaagt gtgaccaaaa
     2881 ctattgcaac cgcaggtaga accgttttct tctctggtgt ggcagtgatg ttagtgctag
     2941 cttcaatgat tttgttcccg cagtatttct taaagtcgtt tgcttacgct ggaattagcg
     3001 ttgtcttctt tgcgattatg gcttcgctct ttgcattgcc agcggtgtta gcgttacttg
     3061 gccatcgcgt tgataaatgg cgaattcgaa agagtgcatt aatcaccaaa gaagatggtg
     3121 cctggtcagt tatcgccaga acagttatgc gtcgcccgct agttttcatt ttggcttcgg
     3181 tggctttact tagtgtgttt gcttatccag ctaaagacgt taaattcggc caagttgatg
     3241 agcgcgtact tccaccaact tcacctgttg tagtagcagc tgatgttagt cgcacccgat
     3301 ttgatggcca agctgctacc ccaattgagt tagtgattcc taaagcaagt gcctcagaat
     3361 ctcaggtaga aactttggcc aagcaattag cggaccttga tggggtggtg agcgtcttaa
     3421 caccgaagtc atttaccaaa gatggtttga ccgcacctct accaatggca ttgccagagc
     3481 aatcaacaac tgatctatat cgaattagcg tggtgcaaga acaatcgccg cgaaatgaag
     3541 caagtgttga gatggttcgg caaattcgtg actttgattt accgcaagga acattagtcg
     3601 gtggcgcatc agcaatttat gttgattcgc aagccggtat ctcagattat ctaccgaaag
     3661 ttattacttg gttgatgatt gcaacgttac tggttctctt tatgtacacc ggaagtattt
     3721 tgcttccaat caaagcagta ttacttaacg tcgcaagtct ggctgccact cttggcgtta
     3781 ttacttacat ctttgaagaa ggtaatttaa tgtggctagt tggtgacttt acggtcacgg
     3841 gaaccgtaga tacctcaact ttggtattag ttgcagtcgt cgcctttggt ttatcaatgg
     3901 actatgaatt gtttatgttg agccgcatta aagaagagca cgataacggt gcagataccg
     3961 aagatgcagt aagttttggc ttgcaacgct ctggtcgaat catcagtgct gctgccctat
     4021 taattgcttt ggtattcatc acttttgtga caagtggtgt tacctcaata aaaatgttgg
     4081 gcttaggtac cgcatttgca attttgttag atgcgacttt aattcgagca attttggttc
     4141 cagcctttat gcgcattgct ggtaagtgga actggtgggc accaaaacct cttgctaagt
     4201 tccatgcacg atttggtatt tctgactaac tagttatggc aatcttgatc gacactgcaa
     4261 tttggccatg gcgagattgg ctttggtgtc acatggttag tgacacttca gttgaagaat
     4321 taaaagagtt tgccagcaaa cttggtattc cagagcgtgg atttcagggc gatcactttg
     4381 atgtaccaga gcacatgcga gaaattgcta tctcgcacgg cgccaaagta gttacttcgc
     4441 gtgaaatcct tcaagcgttg tataaatctg gaattagaaa acgaccaagt gaacgccacg
     4501 gtcgacctaa gaatgtgcca attcatcagc cttaactgat tccatctata ccgtctgcca
     4561 caataagcaa gtgagtaata ccgtcccaaa tgcaccagtc aaccttttaa agtttgccta
     4621 cctttccatt gcaacaggtg ttgttgtatt tagtttgaag ttggttgctt ggtggttcac
     4681 tggctcggtc ggcttactgt ctgacgcgct ggaatcaacg gtaaatattg ttgctgcagt
     4741 aatcgcctta cttgccttac gcactgcaat gaaaccagct gaccaaaaac atcatttcgg
     4801 tcgcggaaaa gcagagtact tctcagcttc agttgaagga tt
//
LOCUS       DBNHVN010000011         2105 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_671109, whole genome shotgun sequence.
ACCESSION   DBNHVN010000011 DBNHVN010000000
VERSION     DBNHVN010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_671109"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1004)
                     /locus_tag="AC16Y1_00185"
     CDS             complement(93..1004)
                     /locus_tag="AC16Y1_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5830918.1"
                     /translation="MIANNSGTLVEVKNLSKVFGKSKNEVRAVTDLSFVAKPGRVTGF
                     LGPNGAGKTTTLRCLLDLVAPSSGEATFNGITYANLTDPAKQVGVSLGFTGIHPGRSA
                     YNHLLVFATAAGIKESRIQEVLELVGLTFAKDRKAGSFSTGMKQRLSLATALLGDPQV
                     LILDEPVNGLDPEGIVEIRQLLRNLAAQGKTVLLSSHVLSEVQQTVDDVVIIAKGRNV
                     RECTLQELIEDTRPVVHVVTPTPEKFQNFGGGQVQAAGDRVAIYDTSLKAVGNFAFVN
                     NIELHELSEELLDLEQVFLELTGGANA"
     gene            complement(1030..1101)
                     /locus_tag="AC16Y1_00190"
     tRNA            complement(1030..1101)
                     /locus_tag="AC16Y1_00190"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1067..1069),aa:Thr,seq:tgt)
     gene            1311..>2105
                     /locus_tag="AC16Y1_00195"
     CDS             1311..>2105
                     /locus_tag="AC16Y1_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5830919.1"
                     /translation="MAAVVFANASRTYPGSDKPAVDKLNLEIKDGEFLVLVGPSGCGK
                     STSLRMLAGLEEVTDGTIRIGDRDVTNLNPKDRDIAMVFQNYALYPHMSVEENMGFAL
                     KIAGVSKDEIHKRVVEAAKILDLEAYLDRKPKALSGGQRQRVAMGRAIVRNPQVFLMD
                     EPLSNLDAKLRVQTRTQIASLQRRLGVTTVYVTHDQVEAMTMGDRVAVLKDGILQQVD
                     TPTNLYNHPVNAFVAGFIGSPAMNLLDLKFDDAGVVIEGAHIPLPSA"
BASE COUNT          601 a          470 c          463 g          571 t
ORIGIN      
        1 ctacggtaat cgcggtaaat aagaaggaag caaaaacaat gccgatccat gttttagtgg
       61 tgaaaacttt taaccattca gctcgcattt gattaagcat ttgcaccacc agttagttct
      121 aagaaaactt gctctaagtc gagtaattct tcgcttagtt cgtgcaattc aatgttgtta
      181 acaaatgcaa aattgccaac ggccttgagt gaggtgtcat aaattgctac ccgatcacca
      241 gcagcctgaa cctggccacc gccaaagttt tggaattttt caggcgttgg agttacaaca
      301 tgcacaactg ggcgggtatc ttcaattaat tcctgcaatg tgcattcgcg aacattcctg
      361 cctttagcaa tgatcaccac atcatcaacc gtttgttgca cttcagacaa aacgtgggaa
      421 gaaagaagta cagttttacc ctgcgcagct aaattgcgaa gtaattgacg gatttcaaca
      481 atgccttcag ggtctaaacc atttacaggt tcatccaaaa tcaaaacttg tggatcacca
      541 agtaatgcag tggcgagcga caaacgttgt ttcattccag tactaaatga ccctgctttg
      601 cgatcttttg caaacgtaag ccctactagc tcaagtactt cctgaatacg actttcttta
      661 attccggctg cagtagcaaa tacaagtaag tggttataag ccgagcgacc tggatgtatt
      721 ccggtgaagc caagtgaaac accaacttgt ttagctgggt cagttaagtt cgcgtaggtg
      781 attccattaa atgttgcttc accacttgat ggggcaacta aatctaaaag acaacgaagt
      841 gttgttgttt tacccgcacc attaggccca agaaatccgg ttacccgacc tggtttagca
      901 acgaacgata aatcagttac cgcgcgaact tcgtttttac ttttaccaaa aactttagaa
      961 agatttttta cttcaactaa ggtgcctgag ttattggcaa tcatttttac ctgatttcaa
     1021 ttttgaacga gccccctgtc cggattgaac ggacgaccgc tcgcttacaa ggcgagtgct
     1081 ctaccactga gctaaggagg ccaagattaa acagttaagt cttaataagt gtttaagccg
     1141 tagaacagtc tatgaattac ttggtttttc ccaaaattct ggtcgtgact gctaattcct
     1201 tgcctagaat taaagggttg caaagaattt tgtaacaact tgtggctgca aagtcccaag
     1261 tcctttacaa cggatcgtcc ggcacgtacc tgccggtgaa ggagaacgaa atggctgctg
     1321 tcgtatttgc gaacgcatca agaacatacc cgggctcaga taagccagcg gtagacaaat
     1381 taaatctaga aatcaaagat ggcgagtttc tcgttttagt tggtccgtca ggttgcggta
     1441 aatcgacttc acttcgcatg ttggctggcc ttgaagaagt taccgatggc acgattcgca
     1501 ttggcgatcg tgatgtaact aacttgaatc caaaagatcg cgacatcgca atggtgttcc
     1561 agaactatgc tctttatccg catatgtcgg tcgaagaaaa tatgggcttt gcgctaaaaa
     1621 ttgctggggt tagcaaagac gaaattcaca agcgagtagt cgaagcagcc aagattcttg
     1681 atctggaagc ctacttagat cgcaaaccaa aagcgctatc tggtggtcaa cgccagcgcg
     1741 tagcaatggg ccgagcgatc gtacgtaatc cacaagtttt cttaatggat gaaccacttt
     1801 cgaacttaga tgccaagtta cgtgtgcaga ctcgtactca gatcgcttca ctacagcgtc
     1861 gcttgggtgt aaccacggtc tatgtaaccc acgaccaagt tgaagcaatg actatgggtg
     1921 atcgcgttgc cgtgcttaaa gatggcattt tgcagcaagt agatacgcca accaaccttt
     1981 ataaccatcc agttaacgca tttgtggctg gctttattgg ttcaccagcg atgaacttac
     2041 ttgacctaaa gtttgatgat gcaggtgtag taatcgaagg tgcacacatc ccgctgccaa
     2101 gcgct
//
LOCUS       DBNHVN010000012         2259 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_225332, whole genome shotgun sequence.
ACCESSION   DBNHVN010000012 DBNHVN010000000
VERSION     DBNHVN010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_225332"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="AC16Y1_00200"
     CDS             complement(<1..153)
                     /locus_tag="AC16Y1_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013399233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HIR5830920.1"
                     /translation="MFNSLSDKFASTFKQLRGKGRLSENDIDETVREIRSALLDADVA
                     LPVVRDF"
     gene            complement(183..938)
                     /locus_tag="AC16Y1_00205"
     CDS             complement(183..938)
                     /locus_tag="AC16Y1_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017956101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR5830921.1"
                     /translation="MDLLSISNTDFLLALLLFFGAILYTSVGHAGSSVYIAIMAVFGL
                     SANVIKPTALILNIFVSSFTSWRYLRAKLFDFKLFIPLAMGAIPMAYIGGGIELSNEV
                     YKPVVGVLLVIAGVNYLFLGKGQIRKDIKAPKFVVALLVGAGIGLLAGLTGTGGGIFL
                     SPIAILLNWSAVKQISGTTSLFILTNSVFGLLGHASSVSQLPNKLPLFVVAVLLGALI
                     GTVLGISKFSNEKVKRALGLVLIIAGLKLALGL"
     gene            complement(944..1180)
                     /locus_tag="AC16Y1_00210"
     CDS             complement(944..1180)
                     /locus_tag="AC16Y1_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006330371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR5830922.1"
                     /translation="MSTIRFFAAARAITKKDQIEVTAQTLGQAMQAAAKLFPELTNAL
                     PKCSYLINGLAAKDLTSALKPSDTIDVLPPFAGG"
     gene            complement(1182..2183)
                     /gene="moaA"
                     /locus_tag="AC16Y1_00215"
     CDS             complement(1182..2183)
                     /gene="moaA"
                     /locus_tag="AC16Y1_00215"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR5830923.1"
                     /translation="MSKLIDTYGRVHRDLRVSLTDRCNLRCTYCMPAEFNNWLPNEDL
                     LTTEELMLVIQTAVNAGITEVRLTGGEPLLRPDIIEIVSRISKLENAPKLSLTTNGIR
                     LAQLAQPLRDAGLARINVSLDTLQPERFNELTHRDRFSDVLVGLQAAKTAGLAPIKIN
                     TVLMPGVNTDEAVDLVKWSLAEGYQLRFIEQMPLDAGGIWDRTNMVTADQIFESLKAE
                     FELTPKQNRGSAPAEEFYVNGGPQTVGIIASVTRPFCGDCDRLRLTSDGQIRACLFSR
                     TETDVRGILRDESILFEDKLTRIEKLFINTVADKLPGHGINNPEFIQPSRPMSAIGG"
BASE COUNT          683 a          507 c          439 g          630 t
ORIGIN      
        1 aaagtcacgt acgacaggaa gtgctacgtc tgcatcgagt aaggcacttc gaatttcgcg
       61 aactgtttcg tcgatgtcat tttcagataa ccgacccttg ccacgcaact gtttaaaagt
      121 tgaggcaaat ttgtcgctaa gtgagttaaa cattagaact aaagtttagc cgaccagtat
      181 ttttagagac ctaaagctag ttttaagcca gcgattatta ataccagacc taaggcccgt
      241 tttacttttt cgtttgaaaa tttacttatc ccaagcactg ttccaatcaa agcgcccaaa
      301 agaaccgcaa ccacaaaaag tggcagtttg ttcggtaatt gactaacgga gctggcatgg
      361 cctagtaggc caaaaaccga gttagtcaga atgaaaagcg aagtggtccc agaaatttgc
      421 ttaacagctg accaatttaa aagtatggct attggggaaa gaaagattcc accgccagtt
      481 ccggtaagcc cagctaataa accaatgcct gccccaacca acaacgccac aacaaatttt
      541 ggcgctttta tatctttgcg gatttggcct ttgcccaaaa acagataatt tactcctgca
      601 ataacgagta gtacgccaac aactggtttg tagacttcat tacttagttc aatcccacca
      661 ccaatgtagg ccattggaat tgcccccatc gctaatggga taaaaagttt aaaatcgaat
      721 aattttgctc ttaaataacg ccaactagta aacgaggaga caaaaatatt tagaatcagt
      781 gcagtgggtt taattacatt ggccgacagg ccaaagaccg ccatgatcgc aatgtaaacc
      841 gatgaaccag catgaccaac tgaggtgtac aaaattgcac caaagaagag taagagtgcg
      901 agcaaaaaat cagtattaga aatagaaagt aaatccactt ggtttaccca cctgcaaatg
      961 gcggcaatac atcaatagtg tcacttggct taagcgcact agttaaatcc tttgccgcca
     1021 agccgtttat taaataacta cattttggta atgcattagt cagttcagga aaaagttttg
     1081 ctgccgcttg cattgcttga cctaaagttt gcgcggtaac ttcaatttga tcttttttgg
     1141 ttatggcccg ggccgcagcg aagaatctaa tcgtgctcac gttagccacc aattgctgac
     1201 atcggccgac ttggttgaat aaattctgga ttgtttattc cgtgtcctgg gagcttatcg
     1261 gctaccgtat taatgaataa cttttcaatt cgagtcaatt tatcttcaaa caaaatactt
     1321 tcatctcgca aaattccccg aacgtcagtt tcagtgcgtg agaaaaggca agccctaatc
     1381 tgtccatcgg aggttaacct caaccgatcg cagtcaccac aaaatggccg agttacgctg
     1441 gcaatgattc ccacagtttg aggtccacca tttacataaa attcttctgc tggtgctgaa
     1501 ccacgatttt gtttcggggt taattcaaat tcagctttta aactctcaaa aatttgatct
     1561 gcagtaacca tattggtgcg atcccaaatg ccgcctgcat ccaaaggcat ctgctcgatg
     1621 aatcgaagtt gatatccttc ggcaagcgac cacttcacta aatcaacagc ttcgtcagta
     1681 tttacacctg gcattaagac ggtattaatt tttattggcg ctaagccggc agtttttgcc
     1741 gcctgtaaac caactaatac gtcagaaaag cgatcacggt gagtcagttc attaaatctt
     1801 tctggctgca aagtatctaa actgacatta attctcgcta agccagcgtc tctaagtggc
     1861 tgggctaatt gcgccagtcg aatcccatta gttgttagcg agagttttgg tgcattttct
     1921 aatttagaaa tccggctaac aatctcaata atgtctggac gaagtagcgg ttcaccacca
     1981 gttaagcgca cttcagttat cccagcattc accgcggttt ggataactag cattaattct
     2041 tcggtagtta ataagtcttc attgggtaac caattattaa actccgcggg catacagtat
     2101 gtgcaacgca aattacatct atctgtaagt gatacccgta gatctcgatg aactcgacca
     2161 taagtatcaa ttaacttact catgcgaaat taacccactc atctgtgcca tcagagaatt
     2221 cctgatgttt ccaaattgga atttgtaact taacttcat
//
LOCUS       DBNHVN010000013         1644 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1711548, whole genome shotgun sequence.
ACCESSION   DBNHVN010000013 DBNHVN010000000
VERSION     DBNHVN010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1711548"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="AC16Y1_00220"
     CDS             <1..282
                     /locus_tag="AC16Y1_00220"
                     /inference="COORDINATES: protein motif:HMM:NF048150.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HIR5830924.1"
                     /translation="VTLDQEALPGILAANGIAEEPLEKLVNNQEVRAIVQTAINTANE
                     AVSLSEAIKKFTILPLDLTIDNGYLTPKMSIRRHVVTSDFAKEISDLYN"
     gene            289..1458
                     /locus_tag="AC16Y1_00225"
     CDS             289..1458
                     /locus_tag="AC16Y1_00225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HIR5830925.1"
                     /translation="MQLKLLGLIAAWLASRAWVLLSGFEILPYPEGANLFADVRLYDW
                     WAGNIQDGHFPINDPMWQYPPLAALVFLLGYLIAPQTIGFVFLATAADALIFYFLLEA
                     GQSRPDKKYLPAVIWVSTAVFMGPVMLGRFDVFPTLFAVLALLFIRNNKYFGINVAIG
                     AILKVWPILLLAAIPKTRFKSTLVWFTLTFGIFAGALTIWWPNSFSFLVGQRSRGLQI
                     ESYGALPYLAWNALVDPLLLEFRYGAVEVLATGVNLVSLLITIAGFALIGQVIYWRLW
                     GKLERVEPSLVALYLVMISMVTSRVLSPQYMVWIFGLLAVCAFASIRKFNLIFWLVAV
                     SAFAGQLIYPIFYFSYMSGDLLALTIQVIRLATLLFATFLVWQNLQQQKNQITTN"
     gene            complement(1455..>1644)
                     /locus_tag="AC16Y1_00230"
     CDS             complement(1455..>1644)
                     /locus_tag="AC16Y1_00230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5830926.1"
                     /translation="VEPGQSGFIVSGTDQDHLVKQIVELLADSELRDRMGTFGRNWVQ
                     TSWTWDQRVQRLNNLISN"
BASE COUNT          420 a          341 c          342 g          541 t
ORIGIN      
        1 gtcacattgg accaagaagc actgcccgga attttggctg ctaacggtat tgctgaagag
       61 ccgcttgaaa aattggtcaa caatcaagag gttcgcgcga ttgttcagac agcaattaac
      121 actgctaacg aagcagtttc gctttctgaa gcaattaaaa aattcacaat tctgccgctt
      181 gatctgacaa ttgataacgg ttacttaact ccaaaaatga gtatccgtcg ccatgtagtt
      241 acttcagatt ttgcgaaaga aatttcggat ctctataact agggattaat gcaacttaaa
      301 cttcttgggc tgattgctgc ctggctagcc agtcgtgctt gggttttact atctggcttt
      361 gaaatacttc cgtacccaga aggtgcaaat ctatttgcag atgtccggtt gtacgactgg
      421 tgggcgggca acatccaaga tgggcacttc ccaatcaatg acccgatgtg gcagtaccca
      481 ccgctagcag cattagtttt tctgcttgga tacctaatcg cgccacaaac tattggtttc
      541 gtatttttag caacggccgc agatgcgtta attttttatt ttttacttga ggctggtcag
      601 agtaggcctg ataaaaaata cctaccggca gtaatttggg taagtactgc agtttttatg
      661 ggaccagtca tgcttggtcg ctttgatgtt tttccaactt tgttcgccgt actagcctta
      721 cttttcattc gaaataataa gtatttcggc ataaatgtag ctattggagc gattttaaaa
      781 gtctggccaa ttttattact cgctgcgata cccaaaactc gatttaaatc tacgttggtt
      841 tggtttacct taacctttgg aatattcgcc ggcgccttaa ctatttggtg gccaaacagt
      901 ttttcatttt tagttgggca acgttcaaga ggtcttcaaa ttgagtctta cggagctctt
      961 ccctatctcg cctggaatgc tttagttgat ccactgctac ttgaatttcg ttatggcgcc
     1021 gtagaagtgc ttgctactgg agttaactta gtttcacttt taattactat cgctgggttt
     1081 gcacttattg ggcaggtaat ttactggcga ctttggggaa aactcgaaag agttgaacca
     1141 agtttggttg cactttattt ggtgatgatt tcaatggtga ccagccgagt actctcaccg
     1201 cagtacatgg tttggatttt tggattattg gcagtttgcg cctttgcctc tattcgaaag
     1261 ttcaatttaa ttttctggtt agtggcagtt agtgcttttg ccggacaatt gatttaccca
     1321 attttttatt tctcatacat gtctggtgac ttattggcat taactatcca agtaatcagg
     1381 ttagctacgc tactttttgc gacttttttg gtctggcaaa atctacagca acaaaaaaat
     1441 caaataacga cgaattaatt cgaaattaaa ttatttagtc gctgtactct ttgatcccac
     1501 gtccagctag tttgcaccca atttcgacca aaggttccca ttcggtcgcg taactctgaa
     1561 tctgcgagta attccacaat ctgtttaact aaatgatctt gatcggttcc agaaacaata
     1621 aacccagatt gcccgggctc gact
//
LOCUS       DBNHVN010000014         5124 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_968614, whole genome shotgun sequence.
ACCESSION   DBNHVN010000014 DBNHVN010000000
VERSION     DBNHVN010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_968614"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="AC16Y1_00235"
     CDS             complement(<1..649)
                     /locus_tag="AC16Y1_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR5830927.1"
                     /translation="MAQRKSLGRGLGALIPDVSRETKKPAKNAPAKKVPAKAPVRAAA
                     KPSVKAPTKTSAPKAVAPAPTEIAGLTFAELNIRSIEPNPKQPRRVFDEEALAELVHS
                     IKEIGLLQPIVVRPSKTGYELVAGERRLRASKKAGLKTIPALIRKTADDQMLRDALLE
                     NLHRSNLTPLEEASAYQQLLSDFGGTQDELAAKLGRSRPQISNTLRLLNLPPAVQR"
     gene            complement(653..1540)
                     /locus_tag="AC16Y1_00240"
     CDS             complement(653..1540)
                     /locus_tag="AC16Y1_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR5830928.1"
                     /translation="MEFQDEDTPLAAAAAAAVYVRRGLKGTFPKPKETRVITIANQKG
                     GVGKTTSAVNLAASMALAGLNVLVIDLDPQGNASTAFGVDHQEGATGTYDVLVDGASI
                     ADMMVAAPKYESLWVLPASLDLAGVDIQLITEVGEKERPFLLKTALDEFLAEHEIDYV
                     FVDCPPSLGLLTINALAAVSEVLIPIQCEFYALEGVQQLLRTIEFAKERLNPDLEVST
                     ILLTMYAQTTNLSLQVSEEVRAYFGKKVLESVIPRSVYVAEAPSFGESVMTYDPGSTG
                     AVAYLAAAKEIADRGRKGR"
     gene            complement(1693..2298)
                     /gene="rsmG"
                     /locus_tag="AC16Y1_00245"
     CDS             complement(1693..2298)
                     /gene="rsmG"
                     /locus_tag="AC16Y1_00245"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007537515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR5830929.1"
                     /translation="MKPEALFSAYPEAVNKLEKFHHWLATAGIDRGILGPRELDRIWD
                     RHIANCAVVEELIPENSMVLDVGSGAGLPGIVLAIVRPDLKVGLIEPLQRRCDFLTEA
                     ITDLGLAGQVQVHRGRAQDHTNLKADVVTGRAVAPLHKFLTWTTPLVKPTGQILAMKG
                     SSAATEITEAKTQLAGKSAEIVQVGAGLVDPLTTVVRVKPE"
     gene            complement(2305..2778)
                     /locus_tag="AC16Y1_00250"
     CDS             complement(2305..2778)
                     /locus_tag="AC16Y1_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Jag family protein"
                     /protein_id="HIR5830930.1"
                     /translation="MSDRIEQLEKEGDIAADYLEGLLDIADMDGDIDMDIDNDRAVVS
                     IVGADLSELVGEKGKVLDAIQELTRLAVTRETGERSRLMLDISGFRVAAKERLVKIGQ
                     DAAKKALESGEPVELKPMSAFERKVVHDAVNEAGAVSVSEGEEPKRRIVVHPAQP"
     gene            complement(2824..3726)
                     /gene="yidC"
                     /locus_tag="AC16Y1_00255"
     CDS             complement(2824..3726)
                     /gene="yidC"
                     /locus_tag="AC16Y1_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIR5830931.1"
                     /translation="MNPLVWLENLVSWILVFFHGLTSIPFDKDSGWSWGLAIVSLVIL
                     IRILLIPLFVKQIKSQRNLQIIQPQVKEIQKKYAGDRERQSQELMKLYKETGTNPLAS
                     CLPILAQAPIFFALFTVLNGLAQSKTRGVLTVELMESARNAEILGAPIYGTFLRRSET
                     AAPSTTLWVTVLLIFLMTATTFISQRQLIVKNTAPGNPMVQQQKILLYVFPVIFAVSG
                     INFPIGVLLYWFTTNLWTMGQQFYVIRNSPQPDTPAYKALMERKARKAAKKNKGQVEQ
                     TPEQPEQPTKQARNQPKKKKKKKK"
     gene            complement(3726..4085)
                     /gene="yidD"
                     /locus_tag="AC16Y1_00260"
     CDS             complement(3726..4085)
                     /gene="yidD"
                     /locus_tag="AC16Y1_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR5830932.1"
                     /translation="MQPTRQVIRKALKATQQVWDQTIGQVLVLVLVGLIRIYQKTLSV
                     WRGPVCRYYPTCSHYGVGALQIHGATKGILLTTGRVLRCHPWSAGGIDLVPTRGNWTY
                     VEPEVIEDSTKLNRQVA"
     gene            complement(4085..4417)
                     /gene="rnpA"
                     /locus_tag="AC16Y1_00265"
     CDS             complement(4085..4417)
                     /gene="rnpA"
                     /locus_tag="AC16Y1_00265"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR5830933.1"
                     /translation="MLPSQVRLRKSLEISRALKVGKRYPAKFLVLHIAPGLTDGTRFA
                     FAVGKNVGNSVVRHKVVRRLRHLTMDNQTRFPSSCDVVVRALPGIDSASHQELQNNFD
                     QALAKVGS"
     gene            complement(4427..4564)
                     /gene="rpmH"
                     /locus_tag="AC16Y1_00270"
     CDS             complement(4427..4564)
                     /gene="rpmH"
                     /locus_tag="AC16Y1_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013289388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR5830934.1"
                     /translation="MSKRTFQPNNRRRAKTHGFRSRMSTRAGRAILSARRGKGRARLS
                     A"
     gene            4875..>5124
                     /locus_tag="AC16Y1_00275"
     CDS             4875..>5124
                     /locus_tag="AC16Y1_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR5830935.1"
                     /translation="MENIDLAQVWESALNSLSSGTLTAQQRAFVALTRPLGLVGDTAL
                     IAAPNEFTKDVLESRLSPVVCDALSKILGRDIRLAVTVD"
BASE COUNT         1227 a         1238 c         1221 g         1438 t
ORIGIN      
        1 gtcgttgcac tgctggtggc aaatttagta agcgcagagt atttgaaatt tgcggacgtg
       61 atcggcccag tttggcagct agttcatcct gagttccacc aaagtcactg agcaactgtt
      121 ggtaagccga agcctcttcg agtggggtta gattcgagcg gtgtaagttt tccagcaatg
      181 cgtcgcgaag catctggtca tcagcagttt tgcggatcag ggctgggatt gttttaagcc
      241 cagctttttt tgaggcccga agtcgtcgct cacctgctac taattcgtag ccagtttttg
      301 atggccgaac cactattggc tgaagtaacc caatctcttt aattgagtga actaactcgg
      361 ctaacgcttc ctcgtcaaat actcgacgcg gctgcttggg gtttggctca attgaccgaa
      421 tatttagttc ggcaaaagtt agtccggcaa tctcagttgg ggctggggcg acggccttcg
      481 gggccgaagt tttggttggg gctttcactg atggcttggc ggcagcccga actggggcct
      541 tcgctggaac tttcttagcc ggtgcattct tagctggttt tttagtttca cgtgaaacat
      601 ctgggatcaa agcacctaaa cctcgcccga gactcttacg ctgtgccatg agttacctac
      661 cctttcgtcc tcggtcggca atctcttttg ccgccgctaa atatgccact gccccagttg
      721 agcctgggtc ataggtcata actgactcac caaatgaagg tgcttcagcc acataaaccg
      781 atcgtggaat tactgactcc aaaaccttct taccaaaata ggcccggact tcttcggata
      841 cctgaagtga caggttcgta gtttgggcgt acatagtcag caaaatagtg cttacttcta
      901 gatctggatt tagccgttcc ttggcaaact cgatagtccg aagtaattgc tggacccctt
      961 cgagggcgta aaactcacat tgaattggga tcagaacctc tgaaacggcg gccagggcgt
     1021 taatcgtcaa aaggcctaaa ctcggcggac aatcgacaaa cacataatca atttcatgtt
     1081 cggccaagaa ttcatctaga gcggttttaa gtagaaatgg ccgctctttt tctccgactt
     1141 cggtgatcag ttggatatcc acccccgcta gatccagtga tgccggaagc acccaaagtg
     1201 attcatattt aggggccgcc accatcatgt cagcgatact ggcgccatca actagaacgt
     1261 cataggtgcc ggtggcgcct tcttgatgat ccaccccaaa tgctgtggag gcatttcctt
     1321 gtgggtccag atcaataact aagacgttca agccggctag ggccatcgag gcagccaagt
     1381 tcacggccga ggtggtttta ccaactccac ctttttggtt tgcgatggtg ataactcgag
     1441 tttctttggg tttaggaaaa gtccctttga gaccgcgtcg aacatagacg gctgcagctg
     1501 cggctgcagc taacggagta tcttcgtctt gaaattccat gcctgcccta aataccttcc
     1561 cagaatacgt gggattaagt tcttagatta ggcgaatctg ccgacaatca agttgtcggg
     1621 tgccacatat tctggtttca cgtgaaacat cgtcagattt atttactgac ttaatgggcc
     1681 gaagtggcca aattattcag gttttactcg aaccacagtc gtcagtggat cgactaaccc
     1741 ggcgcctact tgtactattt cagctgattt tccagctagt tgggttttgg cctcggttat
     1801 ttcggttgct gccgagctac ctttcatggc caaaatttgc ccggttggtt taactagcgg
     1861 ggtagtccag gttaagaatt tgtgcagtgg ggctacggct cgcccagtaa ctacatctgc
     1921 ttttagattg gtgtgatctt gggctcggcc acgatgaact tgaacctgtc cagccagacc
     1981 tagatcggtt atcgcctcgg tgaggaaatc gcagcgtctt tgtagcggct caattaggcc
     2041 aacctttaaa tctggccgaa ctatcgccag tactattcca ggtagcccag ctccactgcc
     2101 tacatctaaa accatcgaat tttctggaat cagttcttcc accactgcac agttagcaat
     2161 gtgacgatcc cagattcggt ctaattcccg gggacccaaa atacctcggt ctatcccggc
     2221 tgtggccaac cagtgatgaa acttttcaag tttgttgact gcctcagggt aggccgaaaa
     2281 aagagcttct ggtttcacgt gaaactatgg ttgggcggga tgaacaacaa ttcggcgttt
     2341 cggctcttcg ccttcagaga cacttactgc cccagcctca tttactgcgt cgtggactac
     2401 ttttcgctca aatgcgctca ttggttttag ttcaactggt tcaccagatt caagcgcctt
     2461 cttggctgcg tcttggccaa tttttaccag gcgctctttg gcggctaccc ggaagcccga
     2521 aatatctagc attaggcgag agcgttcacc agtttcacgg gtaacggcta ggcgagttag
     2581 ttcctggatt gcatccagaa ctttgccctt ttcgccgact aactcagata ggtcggcacc
     2641 aacgatgctg actacggctc gatcgttatc aatatccatg tcgatgtcac catccatgtc
     2701 ggcgatatct aataagccct ctaggtagtc ggctgcgatg tcgccttctt tttctaattg
     2761 ttcaatacga tcactcacgg ttacttacct ttcaataaat ctgatcttgg cttttggcct
     2821 taattacttc tttttcttct tctttttctt aggttggttt cgagcctgct tagttggctg
     2881 ttcgggctgc tcgggagtct gttcaacctg gcccttattc ttcttggccg cctttcgggc
     2941 tttacgttcc atcaaagcct tgtaggcagg ggtgtcaggt tgtgggctat tgcggatgac
     3001 gtagaactgt tgacccatag tccaaaggtt tgtggtgaac caatagagca gcacgccgat
     3061 cggaaagtta atacctgaga cggcgaaaat cactgggaat acgtagagca atattttttg
     3121 ctgctgcacc atcgggttgc caggtgcggt gttctttacg atcaactgtc gttgggaaat
     3181 aaatgtagtt gcggtcatca agaaaattaa aagtacggtg acccagagag ttgtacttgg
     3241 tgcggccgtt tcactccgac gaaggaatgt gccatagata ggcgcgccta aaatttctgc
     3301 gttacgtgct gactccatta actcgacagt taacacgcct cgagttttac tttgcgctaa
     3361 accattcaac acggtgaata gcgcaaagaa aattggtgct tgtgccaaga tcggcaaaca
     3421 gcttgctagc gggttagtgc cagtctcttt ataaagtttc atcaactctt gagattgacg
     3481 ttcacgatcg ccggcatact ttttttgaat ttcttttact tgcggctgaa tgatttgtag
     3541 gttgcgttga cttttaattt gtttaacaaa aagcggaatc aacaaaatac gaatcaagat
     3601 aaccagcgaa acaattgcta gtccccagga ccaaccactg tctttatcga acggaatact
     3661 tgttagtccg tggaaaaaaa cgagaatcca gctcactaaa ttttcgagcc atacaagtgg
     3721 attcattaag caacctgcct attcagttta gttgaatctt caattacttc tggttcgaca
     3781 taggtccagt taccccgagt tggaactaag tcgattccgc cagctgacca agggtgacaa
     3841 cgtaaaactc gcccggtcgt taacagaatt cctttggtgg caccatgaat ttggagggcg
     3901 ccaactccgt agtgtgaaca ggttgggtag taacgacaaa ccggtccgcg ccaaacactc
     3961 aaagttttct ggtaaatccg gattagccca acaaggacta aaactaaaac ttggccgata
     4021 gtttgatccc aaacttgttg ggttgctttc aaggcttttc taattacttg tctagttggc
     4081 tgcattacga cccgaccttg gctaatgcct gatcgaaatt attttgtagt tcttgatgtg
     4141 aggcactatc gattccaggc agtgcccgaa caacaacatc gcaactactt gggaacctgg
     4201 tttggttatc catggttaag tggcgaaggc gccggactac tttatggcgg accactgaat
     4261 ttcccacatt ctttccaaca gcaaatgcaa atctagttcc atcggtaagg cctggggcta
     4321 tgtgaaggac cagaaacttg gctgggtaac gtttgcccac ttttaacgcc cgcgaaattt
     4381 ctagtgactt cctaagccgc acttgtgacg gcaacatcaa taaaacttaa gcagataggc
     4441 gagcgcgacc cttgccacga cgagcagaaa gaattgctcg gccagcgcgg gtgctcatgc
     4501 gactacggaa accgtgggtt ttggcacgtc gacggttatt tggttgaaaa gtacgttttg
     4561 acatttaaat ctctctcgca ggaagtttgg ggctaccgat tcgccatcgg acattcataa
     4621 tttcggtgtc ttttagttac cgactttatg aatattcgac gagtacctat cgatacctcg
     4681 tgcctgttaa agatacgcga atataaaggg tttggtcaaa taaaacccta gatttaacgg
     4741 ccttttagtt aaaggttttt cttgccaaat cagtgggata ttgccagtcc aaccaaatgt
     4801 ggaaagcgac ttgtcagcga ctatccacag gcaatagatt gcttactcct acctgtggat
     4861 atcgagacgg tgaaatggaa aatattgact tagcccaagt ctgggaaagt gccttaaatt
     4921 ccctttcgtc cggcactctc accgcccaac aacgagcttt tgttgcgctt actcgtccac
     4981 ttggccttgt tggtgacact gcgcttattg ccgcacctaa cgaattcact aaagatgttt
     5041 tggagtctcg ccttagtcca gttgtctgcg atgcgctttc aaaaatactt ggccgcgaca
     5101 ttcggctcgc agtgactgtc gacg
//
LOCUS       DBNHVN010000015         4582 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_374494, whole genome shotgun sequence.
ACCESSION   DBNHVN010000015 DBNHVN010000000
VERSION     DBNHVN010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4582)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_374494"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..738)
                     /locus_tag="AC16Y1_00280"
     CDS             complement(112..738)
                     /locus_tag="AC16Y1_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HIR5830936.1"
                     /translation="MTRILVVDNYDSFVYNIVQYLGQLGAKVDVRRNDEVTIEEARSY
                     DGILLSPGPGTPKDAGVCIDIVKELGGEIPIFGVCLGHQAIAEAFGATVSRAPELLHG
                     KTSEVFHDGVGVLAAVNSPFTATRYHSLAVERDTVPDVLEITGQTSGGVIMGLRHKNK
                     DIEGVQFHPESVLTQGGHQMLAEWLVRCGDLEARNRAVGLAPVINKTN"
     gene            complement(750..2489)
                     /gene="pknB"
                     /locus_tag="AC16Y1_00285"
     CDS             complement(750..2489)
                     /gene="pknB"
                     /locus_tag="AC16Y1_00285"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HIR5830937.1"
                     /translation="MVSQFDRLGDRYEIKQIIGRGGMAEVHEGLDTRLNRRIAIKVLR
                     ADLARDPIFIERFRREAQSAAGLNHPNIVSIYDTGEDSHTEGVTQTNVPYIVMEFVDG
                     VTLREMLQNGPRILPERGLEILAGVLAALDYAHRHGIVHRDIKPANVMINTHGDAKVM
                     DFGIARAMSDAATSVTATSAVMGTAQYLSPEQARGEVADARSDIYAAGCLLYELLTGS
                     TPFTGDSPVSIAYQHVNSQVTAPSHLDPSIPPTLDAIVLQALAKNPTSRYQTAAEMRA
                     DVERAIAGMPISAAANTQVFAQGAPTTAIPIVEMENQIDAGQTKKTKQRSKKFKIGSA
                     ITGMIAAVTAVFLLASTLFGGGGGNNATVPDLRGKTTAEAQLLLESVGLTVGTQTPTP
                     DDNAIKGTIVGQDPAAGERLEKGQAVNIKVSIGKGETLVPDLIDLSSTNDARAVLAAS
                     NLVLGKIIQKDSDKPEGTILEQNPIAGSSVEAGSRVDVAISSGKVTVPNVLGLNRTEA
                     YTILFTAGFTVQFIEEPRADIAAGTVLAQSPLPGEVTVKGTIVVLNVASAAPAPAPTV
                     TVTATPSPTATTP"
     gene            complement(2536..3987)
                     /locus_tag="AC16Y1_00290"
                     /pseudo
     CDS             complement(2536..3987)
                     /locus_tag="AC16Y1_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
     gene            complement(3984..>4582)
                     /locus_tag="AC16Y1_00295"
     CDS             complement(3984..>4582)
                     /locus_tag="AC16Y1_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006437477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR5830938.1"
                     /translation="LYVATGRRAWVVIGALLLAIGGVLAYLNFSHVQTRFSVWLDPFA
                     DAEGRGYQIVQSLYGLANGGIFGTGWGRGYPQLVPFAKTDFIASALGEELGLTGLIAI
                     FILYAIIVERGARAAVATRDPFGNLLATGLSAVIGLQAFIVIGGVTRLIPMTGLTTPF
                     LSYGGSSLISNYILIAILIRISNAARAPEVEQQKVTSP"
BASE COUNT         1254 a         1083 c          984 g         1261 t
ORIGIN      
        1 taaatgaaga accaggtgta aagacttatc gtcaatacgt tgatcgagtt aacttgggct
       61 gggaagtaaa tgtatttaat gaatacacgc ttccagcgtg gcaaggttcg cttagttagt
      121 tttattaatt accggcgcta aaccgacagc tcggttgcgt gcttctaaat cgccacaccg
      181 aactaaccat tcggctagca tttggtggcc cccttgcgtt aaaactgatt caggatgaaa
      241 ctgcacacct tcaatatctt tatttttgtg acgcagtccc ataattacgc cgcctgaagt
      301 ctgtcctgtt atttctagta catccggaac ggtgtctcgc tcaacagcca gtgagtgata
      361 tcgagttgcc gtaaatggtg agttaactgc tgctaaaaca ccaacgccat catgaaatac
      421 ttctgatgtt tttccgtgaa gtaattcagg tgctcgtgag actgttgcgc caaaagcctc
      481 tgcaattgct tgatgtccta aacaaactcc gaatatagga atttcaccgc caagctcttt
      541 gacgatgtca atacaaacgc ctgcgtcttt aggcgttcca gggccgggtg aaagcaaaat
      601 accatcgtaa ctgcgagctt cctcaattgt tacttcatca tttcgacgaa catccacttt
      661 ggctccgagt tgacctaagt attgaacaat gttgtaaacg aaactgtcgt agttatcgac
      721 aactaaaatg cgagtcattt aatttccggt tatggagttg tcgcagttgg ggaaggagtt
      781 gcagtgaccg taacggtcgg tgccggcgct ggtgctgccg atgcaacatt gagtacaaca
      841 atcgtgcctt taacggttac ttcaccaggt agtggtgatt gagctagtac agttccggca
      901 gcaatgtcag cccttggttc ctcgataaat tgaacggtga aacctgcagt aaacaaaatt
      961 gtgtaggcct ctgttcgatt aagaccaaga acattaggca ctgtcacttt gccgcttgaa
     1021 attgcaacgt caacgcgtga gccagcttca actgaacttc cagcaattgg attttgttcc
     1081 agaatcgtgc cttcaggttt gtcactatct ttttgaatta tttttccaag caccagattc
     1141 gaagctgcta agacagctcg agcatcgttt gtactactca aatcaattaa atctggaact
     1201 aaagtttcac ctttgccaat cgaaacctta atgtttaccg cttgcccttt ttcaagacgt
     1261 tccccagcag cgggatcttg gccaacgatt gtgcccttaa ttgcattgtc atctggtgtc
     1321 ggtgtttggg taccaactgt taaaccgaca ctttcaagca aaagctgagc ttcagcagta
     1381 gttttgccac gtaggtctgg aactgttgcg ttgttaccgc caccgccacc gaataaagtg
     1441 ctggcaagta aaaatactgc agttaccgct gcgatcatac ccgtgattgc actgcctatt
     1501 ttaaatttct ttgagcgctg ttttgttttt ttagtctgac cagcgtcaat ttgattttcc
     1561 atctcaacaa ttggaattgc tgtagttggc gcaccttgag cgaacacttg cgtatttgca
     1621 gcagcactaa ttggcatccc agcaatcgcg cgttcaacat ctgcacgcat ctcagccgcc
     1681 gtttgatatc tacttgttgg attcttagcg agtgcttgca agacaatcgc atcaagagtt
     1741 ggcggaatac ttggatctaa atgagatggc gccgtaactt gtgaatttac atgttgataa
     1801 gcgattgaca caggtgagtc accagtgaat ggagtactac cagttagtaa ttcataaagt
     1861 aagcagccag cagcataaat atcacttcgg gcatcagcaa cttcaccgcg tgcttgttcc
     1921 ggagatagat actgagctgt tcccatcact gcgctcgttg cagttaccga agtagccgca
     1981 tcactcattg ctcgagcaat tccgaagtcc ataactttgg catcgccgtg ggtgttaatc
     2041 attacatttg ctggctttat gtcacgatga acaattccat gacgatgcgc ataatctaaa
     2101 gccgctaata caccagcgag aatttctaaa ccacgctctg gcaaaatacg tgggccattt
     2161 tgtaacatct cgcgaagggt cacaccatcg acaaattcca tgacgatgta aggcacattg
     2221 gtttgagtaa cgccttcggt gtgcgaatcc tcaccagtgt cgtaaattga aacaatattt
     2281 ggatggttta acccagcagc tgattgagct tcacgacgaa aacgttcgat gaaaatgggg
     2341 tctctggcta aatcagcacg caaaactttt attgcgatcc gacggttaag tctggtgtct
     2401 aggccttcat gcacctcggc cataccacca cgaccaatga tctgtttgat ttcgtaacga
     2461 tcacctaatc ggtcgaactg actcaccatt tctccttgct aaacatgggc ctaccacgtc
     2521 tgctactaac taacactacc tattactcag gattgcttgc attaccgcac gtgcgattgg
     2581 cgcggccaaa gcgttaccac taacttcagt tgctccacca ccgttttgca ggacaactgc
     2641 tactgcaaca tttgcatttt gggcaggcgc tagtccaaca aaccatgcgt gagcagattc
     2701 accaggaaca ttttctgcag ttccagtttt tccaccgacg ctaacgcccg agattcgcgc
     2761 gttagatccg gtgccgtttt cgactacacc aaccatcatg tctcgtaata actttgcatt
     2821 ggttggtgtc attgctcggc taattgcttt gggcttgaag ctttctaaaa tagctaagtc
     2881 agttccaagt gtttctgaaa ttaaatatgg cgacatcaca acgccatcat ttgcaattgc
     2941 tgctgccacc attgccattt gaagtgcagt tgcgcgaaca tcgaattgtc cgatcgcact
     3001 cattgcagtt tgtggctcat ctggatctat cggaaagtaa ctagttgcgg ctacaagtgg
     3061 aatatcaaat tcagtaccaa acccaaattt ttcggcttgg gcgagtaagg catccggacc
     3121 aagttgtaag ccgagccatg cgaaagcagt attacatgag actgccaatg catcagccaa
     3181 agtagtttcg tcatttggcc cacacgattt atcattccaa ttacccaaaa cacgatcagt
     3241 tagtggaagt tgaaattatt taggtccggg taagacactt tcgttggtga agtttcctgt
     3301 ttcaagggca gcagcagctg ttaccaattt aaaaacactg cctggaggca aagtcatcgc
     3361 aatgggacga tttaacatcg gctgatcagg atcagccatt aacttttcat actgcgaagt
     3421 tatatccgcc gaattatgac ttgaaagtaa atttggatca aatgatggtg aactagctaa
     3481 tgccaaaatt gcaccggtgc ttggatcaat tgcgactaca gcgccagtcc ttccattcaa
     3541 acctttcata gccgcaactt gagcatctgc atccaaagtc agtcgaacgg cgccaccttg
     3601 gggttttcga tttgcaaaca tttgttgtag acgatcaaca aaaaatctgc tgtcattgcc
     3661 agccaaaata tcgtttgctt ttagttcaac acccgtagct ccgtatacca aagaataaaa
     3721 tccggttgca gccgcataag tctgaccatc ttgataagtg cgcaaaaact ttaaatcatt
     3781 gtttgtcgga atcgattggg cgattggctc tgagccaatc agaattactc cacgttgacg
     3841 cgagtattca gtcagtaaca tgcgttgatt acctggctta ttacgcaact cgtctgcttg
     3901 aaaaaattgc aaaaaggaaa cattgagcat taaagcaagg attagtaaaa ctaaaaccgc
     3961 ggttactcta tttatttgtc gaatcatggt gaagtcactt tttgttgttc gacttcgggt
     4021 gctcgagctg catttgaaat tcgaatcaaa attgcaatca aaatgtagtt tgaaatcagt
     4081 gacgatccac cataggaaag aaaaggtgtt gttaagccag tcattggaat taatcgggta
     4141 acgccaccga tcacgatgaa tgcttgtaaa ccaataaccg cagaaagtcc agttgccagt
     4201 aggttgccaa acggatcacg tgttgccact gctgctctag ctccgcgctc aacgatgatc
     4261 gcgtaaagaa tgaaaattgc aatcagtccg gtcaaaccaa gttcttcacc aagagccgat
     4321 gcgatgaaat ctgttttggc aaaaggtact aactgcggat agccacggcc ccaaccggtg
     4381 ccaaaaattc caccgttggc tagtccatag agtgattgaa ctatttgata gccacgtccc
     4441 tctgcatcag caaatggatc tagccaaaca ctaaatcttg tttgaacgtg actgaaattc
     4501 aaataggcga gtacgccacc tattgccagc agcagcgcac caataacaac ccaagcccgt
     4561 cgtccagttg ccacatacaa aa
//
LOCUS       DBNHVN010000016         4011 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1117794, whole genome shotgun sequence.
ACCESSION   DBNHVN010000016 DBNHVN010000000
VERSION     DBNHVN010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1117794"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="AC16Y1_00300"
     CDS             <1..511
                     /locus_tag="AC16Y1_00300"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HIR5830939.1"
                     /translation="TFHPTFLYESLACIAIAAILIWEDKRFVLGHGRVFALYVALYCA
                     ARGAIETLRIDQATYILGIRLNVFTALIIGAGGLIYFILSAERYPGREEVANGKLVRA
                     GSRRSRRIENADASQPVAENQIETNNETESQVDQLEADFVPNEVQFEQNSNVEAQDMS
                     KERGRRRKR"
     gene            545..1972
                     /gene="pyk"
                     /locus_tag="AC16Y1_00305"
     CDS             545..1972
                     /gene="pyk"
                     /locus_tag="AC16Y1_00305"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR5830940.1"
                     /translation="MRRAKIVCTLGPSTGTPDAIRELIISGMNVARLNMSHGTHEVHA
                     KSYAMVRQLADETSRGVGILADLQGPKIRLATFASGPVDVFNGDKFTITTREVPGDKE
                     ICGTTYAGLPGDCKPGDLLLIDDGKVTLEVTQVTDTDVVTKVIEGGALSNNKGINLPG
                     VAVSVPALSEKDIDDLRWALRQGVDMVALSFVRSAKDIDDVHQIMNEEGIRIPVIAKI
                     EKPQAVKNLEEIINAFDGIMVARGDLGVELPLEQVPLVQKEAISLARAAGKPVIVATQ
                     MLDSMITMSRPTRAEASDVANAILDGADALMLSGETSVGEHPSLVVQTMSRIIEHIET
                     EALGRLPKISDDGTDSTALALTAAAVDVAIHIHARSLVVFTETGRSVLQVARHRPETG
                     ILAFTPNPRVRNQLSLAWGTETFLVPQVHHTDDMVEQIDRTLLDLGRFAPNEYVVVIA
                     GVPPGVPGTTNGMRVHQLGRGARNL"
     gene            2070..2645
                     /gene="pyrR"
                     /locus_tag="AC16Y1_00310"
     CDS             2070..2645
                     /gene="pyrR"
                     /locus_tag="AC16Y1_00310"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017617047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIR5830941.1"
                     /translation="MTPTAKPAESGNSNNSRVVLDSTDISRAITRLAHEIVERTHGAS
                     NLTLLGIPTRGAALANRLAEKIEQIQGSSISVGAIDVTMFRDDLRLRPARALLPTKIP
                     AEGIDGRLVVLVDDVLYSGRTIRAALDAISEHGRPQAVQLAVLVDRGHRELPIRADYV
                     GKNLPTSSKEVVRVRLVEHDEIDEVAIEGGR"
     gene            2642..3574
                     /locus_tag="AC16Y1_00315"
     CDS             2642..3574
                     /locus_tag="AC16Y1_00315"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003956408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIR5830942.1"
                     /translation="MKHLLSAGDLSRDEAITILDTATQLAQATEQGVGKLPPLRGRTV
                     VNLFFEDSTRTRMSFEAAAKRLSADVINFSAKGSSVSKGESLKDTALTLEAMGADAVV
                     IRHNSSGAPHRLANAGWISGGVVNAGDGTHEHPTQALLDAYTLRKHLRDGQGDLANVK
                     VTIVGDILHSRVARSNVLLLHTLGAKVRLVAPPTLLPIGVESWGCEISYDLDTSISDS
                     DAVMMLRVQRERMNQAYFPNSHEYSNRYGLTKARMNAMAEHAIVMHPGPINRGMEISA
                     DVADSSRSVVVEQVANGVSVRMAVLYLLLGGAEI"
     gene            3583..>4011
                     /locus_tag="AC16Y1_00320"
     CDS             3583..>4011
                     /locus_tag="AC16Y1_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020216383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5830943.1"
                     /translation="MGSIVLKNVSLLGEQVTDILLIDGVISQIGSNLTGDQQVDGTGC
                     VALPGLVDLHTHLREPGREDAETVLSGSRAAAKGGFTCVHAMANTNPVADTAGVVEQV
                     WRLGQDAGLVDVRPVGAVTKNLEGIALAELGAMADSAALVR"
BASE COUNT         1077 a          898 c          986 g         1050 t
ORIGIN      
        1 aactttccat ccaacttttt tgtatgaatc actagcgtgc attgccatcg cagccatttt
       61 gatctgggaa gataagcgat tcgtccttgg acacggacga gtttttgcgc tgtatgtagc
      121 cctctattgc gctgcacgtg gtgcgatcga aactctacgc attgatcaag ccacttacat
      181 tttgggtatt cgcctaaatg tattcactgc gctaattatt ggtgccggtg ggctgattta
      241 tttcatcctt tccgcagagc gctacccagg ccgagaagaa gttgctaatg gcaagttagt
      301 gcgtgcgggt tcacgccgat ctcgtcggat cgaaaatgca gatgcttcac aaccagtggc
      361 tgaaaatcag attgaaacaa ataacgaaac tgagtcgcaa gtagatcaac ttgaagctga
      421 ctttgtgcct aacgaagtcc agttcgaaca aaattcaaat gtggaagcac aagacatgtc
      481 aaaagaacgt ggtcgcagaa gaaagcgcta aactggcaac tgacttaatt taaaggatta
      541 acaagtgcgc cgcgccaaaa tagtttgtac cttaggccct tctactggaa ctccggacgc
      601 catccgagaa ttaattattt cggggatgaa tgttgcccga ttaaatatgt cgcatggcac
      661 ccatgaagtg cacgctaagt cttatgccat ggttcgccaa ctggcagatg aaacatcacg
      721 cggtgttggt attttggccg atttacaggg accaaaaatt cgtttagcca cttttgccag
      781 tgggccagtt gatgttttca atggcgataa attcaccatt actactcgtg aagtacctgg
      841 cgataaagaa atatgtggta ctacttatgc cggcttgcca ggggattgca aacctggcga
      901 tctattgctc atagatgatg gaaaagtaac gctagaggtt acccaagtta ccgacaccga
      961 tgtcgtcacc aaagttattg aaggtggagc tttaagtaat aacaaaggga ttaatttacc
     1021 tggtgttgca gtaagtgtcc cggcgctttc cgaaaaagat attgatgatt tacgatgggc
     1081 gcttcgccaa ggcgttgaca tggtggcttt gtcttttgtt cgcagtgcta aagacataga
     1141 tgatgtgcac cagattatga atgaagaagg tattcgcatc ccggtcattg ccaaaattga
     1201 aaaaccacag gcagtaaaaa atctcgaaga aatcatcaat gcattcgatg gcattatggt
     1261 ggctcgaggt gacttgggag ttgagttacc gcttgaacaa gttccactag ttcaaaaaga
     1321 agctatctcg ctagcacgtg ctgctggaaa gccagtcatt gtggctacgc aaatgctcga
     1381 ttcgatgatt actatgtcca gaccaactag ggcagaagca agtgacgtgg ctaatgcaat
     1441 ccttgatggt gctgatgcgt taatgctctc aggtgaaaca agtgttggtg aacacccttc
     1501 gttagtagtt caaactatga gtcgcattat tgagcacatc gaaacagaag cactgggtcg
     1561 acttccgaaa ataagcgatg atggaactga ttcaacagcg cttgcgttaa ctgcagctgc
     1621 ggttgatgtg gctattcaca ttcatgcgcg cagtttggtg gttttcacgg aaactggtcg
     1681 ttcggttttg caggtagcta gacaccgtcc ggaaacaggt attttggctt tcacgccgaa
     1741 tccaagagta cgtaaccagt tgtcgctggc ttggggaact gaaactttct tagtgccgca
     1801 ggtacatcac accgatgaca tggtggaaca aattgaccga accctattgg acttaggtcg
     1861 ttttgcacct aatgaatatg ttgttgttat cgccggtgtc ccacccggtg tgcctggaac
     1921 aactaacggt atgcgcgtcc accaactcgg ccggggcgct cgtaaccttt agtcccaccc
     1981 gttagagaag atctgataca gtaactgaag ttctagcgaa cgcccatcct ttaagacctg
     2041 tcctgtgagg cagaaaagga ggtaagaaga tgacgcctac cgctaaacct gccgaatccg
     2101 gcaattccaa taattctcga gtagttcttg attcaactga catctctcgc gccataactc
     2161 gtttagcgca tgaaattgtt gaacgcaccc acggtgcatc gaatttaact ctgcttggga
     2221 ttcctactcg cggcgcagct cttgccaacc gcttagctga aaaaattgaa caaatccaag
     2281 gtagctcgat ttcggttggc gccattgatg tcacaatgtt tcgcgatgac ttacggttac
     2341 ggccagctcg cgcattactt ccaaccaaaa ttcctgccga aggtattgat ggacgacttg
     2401 ttgtcttagt cgatgacgtt ctctattcag gccgtactat tcgcgccgca cttgatgcga
     2461 taagtgaaca tggtcggcca caggctgtgc agttagctgt tttagttgat cgtggtcacc
     2521 gcgaacttcc aatccgcgct gactatgtcg gtaaaaatct gccaacttct agtaaagaag
     2581 tggtccgagt tcgcttagtt gaacacgacg aaattgatga agtcgcaatc gagggtggcc
     2641 gatgaaacac cttcttagtg ctggcgattt atctcgcgat gaggccatca ctattttgga
     2701 tactgccact caattagccc aagcaactga acaaggtgtg ggtaaattac ctccacttcg
     2761 aggccgcact gtcgtgaact tattcttcga agattccact cgaacacgaa tgtcttttga
     2821 agcagcagca aagagattaa gtgctgacgt aatcaatttt tccgctaaag gttcaagtgt
     2881 ttctaaaggt gaaagcctta aagatacggc gctaactctt gaagcgatgg gcgctgatgc
     2941 cgttgtcatt cggcacaaca gcagtggtgc gccacatcgt ctggctaatg ccggctggat
     3001 ttccggcggc gtggttaacg caggggatgg cacccatgaa cacccaacgc aagcattact
     3061 agatgcctac acacttcgca aacaccttcg cgatggacaa ggcgatttag caaatgtgaa
     3121 agtcaccata gttggtgaca ttttgcatag tcgagttgcc cgctcaaatg tcttacttct
     3181 gcacacactt ggggcaaaag ttcgattggt cgcaccgcca accttgctac caatcggtgt
     3241 cgaaagttgg ggctgcgaaa tttcttatga tctagatact tcaatttcag actcagatgc
     3301 agtcatgatg ttgcgggtgc agcgtgaacg aatgaaccaa gcatattttc caaattcaca
     3361 cgaatacagc aatcgctatg gcttaaccaa agctcgcatg aacgctatgg ccgaacatgc
     3421 aattgtgatg cacccaggtc caataaaccg agggatggaa atttctgctg atgtggccga
     3481 ctccagtcga tcagttgtcg ttgagcaagt ggcaaatggt gtgagtgtgc gaatggcagt
     3541 gctttattta ctactaggtg gagcggaaat ttaatgaaat caattggctc aatagtgctt
     3601 aaaaatgttt ccctattagg cgaacaagta actgacattt tgttaattga cggtgtgata
     3661 agccaaatcg gttcaaactt aactggcgat caacaagtag atggaacggg ttgtgttgca
     3721 ctacccggtt tagttgactt acatacacat ttacgtgaac caggtcgcga agacgcagaa
     3781 acagttttat cgggttcacg agccgccgca aaaggtggct tcacgtgtgt acatgcgatg
     3841 gcaaatacaa atccagttgc agataccgct ggtgtagtcg aacaagtttg gcgccttggt
     3901 caagatgcag gcttagtcga tgtgcgtcct gttggcgcag tcacaaaaaa cttagaagga
     3961 attgcacttg ctgaacttgg tgcgatggca gatagcgccg cacttgttcg a
//
LOCUS       DBNHVN010000017         2216 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1489193, whole genome shotgun sequence.
ACCESSION   DBNHVN010000017 DBNHVN010000000
VERSION     DBNHVN010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1489193"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="AC16Y1_00325"
     CDS             complement(<1..963)
                     /locus_tag="AC16Y1_00325"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIR5830944.1"
                     /translation="MSKIKVQGTVVDLDGDEMTRIIWSFIKEQLILPYLDVNIDYYDL
                     GIENRDATDDQVTIDAANAIKKHGVGIKCATITPDEARVKEFGLKKMWKSPNGTIRNI
                     LGGVIFREPIIISNIPRLVPGWTKPIIVGRHAFGDQYKATDTKIPGEGTLTLTFTPKD
                     GSAPMELNVFEFPSSGVALAMYNLDESIREFARASMRYGLSRNYPVYLSTKNTILKAY
                     DGRFKDIFQEVFENEFKSEFEKAGITYEHRLIDDMVASALKWEGGYVWACKNYDGDVQ
                     SDTVAQGFGSLGLMTSVLMTPDGKTIEAEAAHGTVTRHFRQHQQG"
     gene            complement(980..1930)
                     /gene="mdh"
                     /locus_tag="AC16Y1_00330"
     CDS             complement(980..1930)
                     /gene="mdh"
                     /locus_tag="AC16Y1_00330"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020215330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR5830945.1"
                     /translation="MAGKVTVVGAGFYGSTTAQRIAEYNIVDTVVLTDILEGKPEGLA
                     LDLNQSRSIEGFETKLIGQTTTPDGQGYEAIAGSDVVVITAGLPRKPGMSRMDLIETN
                     AKIVRSVAENVAKHAPNSVIIVVSNPLDEMTALAQLASGFPKERVMGQAGMLDTARFT
                     NFVAEELKVPVASVKTLTLGSHGDTMVPVPSRCTVDGKPLSDLLSAQKIEELVVRTRN
                     GGAEVVALLKTGSAYYAPSAAAARMAKAVIEDSGAVMPVCAWVDGEYGINGVYLGVEA
                     QIGKGGIKKVVETKLSDHELAGLKEAAEAVREKQADVKDL"
     gene            complement(2032..>2216)
                     /locus_tag="AC16Y1_00335"
     CDS             complement(2032..>2216)
                     /locus_tag="AC16Y1_00335"
                     /inference="COORDINATES: protein motif:HMM:NF022668.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3017 domain-containing protein"
                     /protein_id="HIR5830946.1"
                     /translation="NFRTGAIGLALCVLLAFLLRFYLPDAQAGWLKSRRRRIDLTFLA
                     ALGVALLILALVVPYR"
BASE COUNT          587 a          515 c          443 g          671 t
ORIGIN      
        1 accttgttga tgttggcgaa aatgacgagt cacagtgcca tgagcagctt cggcttcaat
       61 tgttttacca tctggtgtca tcaagacact tgtcattaaa cctagcgatc caaaaccctg
      121 tgcgactgtg tcactttgca catcaccgtc gtagttcttg caagcccaaa catagccgcc
      181 ttcccactta agtgcactag ccaccatgtc atcgattaac cgatgttcgt aagttattcc
      241 agctttttca aattcagatt tgaattcgtt ctcaaatact tcttggaaaa tatctttaaa
      301 tcttccgtca taagctttaa gaattgtatt ttttgttgat aagtagactg ggtaattcct
      361 acttaagccg taccgcatac ttgcgcgagc aaactctcga attgattcat ctagattgta
      421 catagcaagt gcgacgccac tgctgggaaa ttcaaataca ttcaattcca ttggtgcact
      481 gccgtctttg ggagtgaaag ttaaagttaa agttccttca cctggaattt tggtatctgt
      541 agctttgtat tgatcaccaa aagcatgtcg accaacaata atcggtttag tccatcccgg
      601 aactaagcgc ggaatgttgc tgataataat tggttcccga aaaattacgc cacctaaaat
      661 attgcgaatt gttccattgg gtgacttcca catttttttc aagccaaatt cttttacccg
      721 agcttcgtca ggggtgattg ttgcacactt aattccaacg ccgtgctttt ttattgcatt
      781 agcggcatca attgttactt ggtcatcggt ggcgtcacga ttctcgatac caaggtcgta
      841 gtaatcgatg tttacatcta aataaggaag gattagctgc tcttttataa aggaccaaat
      901 tattctggtc atttcgtcgc catcaagatc gactactgtc ccttgaactt ttatttttga
      961 catactcagc atcttttctt tataaatctt taacgtctgc ttgtttttcg cgaacagctt
     1021 ctgcagcttc ttttaaccca gcaagttcat ggtctgaaag ttttgtttca actactttct
     1081 taatgccacc cttaccaatt tgggcttcga cgccaaggta gacaccatta attccgtatt
     1141 cgccatcaac ccaagcgcag actggcatta ctgcaccaga atcttcaata acagctttag
     1201 ccattcgagc tgctgctgcc gaaggagcgt agtaggcaga tcctgttttt aaaagtgcaa
     1261 ctacttcagc gccaccattt cgagtgcgaa caactaattc ttcaattttc tgcgcagata
     1321 gcaagtcgct cagcggtttg ccatctactg tgcagcgaga tgggaccgga accatggtgt
     1381 caccgtgtga accaagggtt agagttttta ccgaagcaac tgggactttt aattcttcgg
     1441 ccacaaaatt tgtaaatcgc gcggtgtcca gcattccggc ttgacccatt actcgctctt
     1501 ttggaaaacc acttgctaat tgagcaagtg cagtcatttc atctaacgga ttagaaacga
     1561 cgataatgac tgagtttggt gcatgtttgg ctacattttc agcaactgaa cgcacgattt
     1621 tggcattggt ttcaattaag tccatgcgac tcatgccagg cttgcgtggt aaaccggcgg
     1681 tgataacaac aacatcagat cctgctatcg cttcatatcc ctgaccgtca ggtgtagttg
     1741 tttggccgat caattttgtt tcaaagcctt caattgatct tgactgattt aagtcaagag
     1801 ctaatccttc tggtttgccc tcgagaatgt cggttagcac aacagtgtct acgatgttgt
     1861 attcagcaat gcgctgagcg gttgttgagc cataaaatcc tgcacccaca acggtaactt
     1921 ttccagccat ttttgattgc gacccttcag taatctaagt cagactttga aatgtcttga
     1981 cctcaagata tctaaattct acggcttaca atttttttcg agtaactgcg gttaccggta
     2041 aggaactacc agtgccagga tcaataacgc cacacccaaa gcagccagaa acgttaggtc
     2101 gattctgcgc cgtcgagact tgagccaacc agcttgggca tcaggtaaat aaaaacgtaa
     2161 aagaaaagcg agcagcacgc aaagtgctaa tccgattgct ccagttctga agttca
//
LOCUS       DBNHVN010000018         1432 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_449142, whole genome shotgun sequence.
ACCESSION   DBNHVN010000018 DBNHVN010000000
VERSION     DBNHVN010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_449142"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="AC16Y1_00340"
     CDS             complement(<1..581)
                     /locus_tag="AC16Y1_00340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR5830947.1"
                     /translation="MKICVCIKQVPDSWAEKKLNPADNTLDRAAADRVVNELDEYAVE
                     EALRLVEAHGGEVTILSVGPTEAADSIRKAISMGATDGIHIVDDAIHGSDAVATSLVI
                     AEALKDKNFDLILFGSESTDARMSVVPAMVAARLDLPQMTFASKVAISGSELTIQRQT
                     DYGYDEVKSTIPAVVSVVEKINEPRYPSFKNIMA"
     gene            complement(668..>1432)
                     /locus_tag="AC16Y1_00345"
     CDS             complement(668..>1432)
                     /locus_tag="AC16Y1_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005566345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR5830948.1"
                     /translation="FISLEHHGSVGLIRLDRPKMNVLNRAMQEEIRLISNEVSTNPDI
                     RTVVIYGGEKSFAAGADIKEMVNWNCADAARETPGLQDCFTAVAKIPKPTIAAVSGYA
                     LGGGLELALSCDFRIASSDAQLGLPEVLLGVIPGAGGTQRLARLIGSSKAKELIFTGR
                     FISGTEAQVIGLVNEVVSPEQLLNRALEFAAALAKGSATALAAAKEAIDQGLNETLPD
                     GLKVEQEIFANLFGTADQIIGMQSFIENGPGKATFS"
BASE COUNT          374 a          354 c          281 g          423 t
ORIGIN      
        1 gccatgatgt tcttgaatga tgggtaccta ggttcattaa ttttctcaac aactgaaact
       61 actgctggaa ttgttgattt gacttcgtcg tatccatagt cagtttgacg ctgaatagtt
      121 aactcactgc cagatatcgc tactttgctt gcaaatgtca tctgcggcag atcgagtcga
      181 gcagctacca ttgccggcac cacactcatt cgtgcgtcag ttgattcact accaaataaa
      241 atcaaatcaa agtttttatc ttttaatgct tctgctatca ccagcgaagt tgcaacggca
      301 tctgagccgt ggatggcatc gtcgacaata tgtattccat ctgtagcacc cattgaaatt
      361 gcttttctaa tgctgtctgc agcttcagtc ggcccaacag aaagaattgt tacttcgcca
      421 ccatgtgctt caactaagcg cagtgcttct tcaacggcat actcatctag ttcatttaca
      481 actcgatcgg ccgcggcgcg atctaatgta ttgtcggcag gatttaattt cttctctgcc
      541 caactgtctg gaacttgttt aatgcacacg caaatcttca aggcgctatc tccttttata
      601 tctagtgccc gaaactagat cacttttaaa ttgtatccaa tcctgcgaat agcgctttaa
      661 gtgggtatta actaaaagtt gccttgcccg gcccgttttc gataaatgac tgcatcccaa
      721 taatttggtc ggccgtgcca aacaagttgg caaaaatttc ctgttcaact tttagtccat
      781 caggaagtgt ttcatttaac ccttgatcga ttgcctcttt ggcagcagct aatgcagtgg
      841 ctgagccttt tgccaaggcg gctgcaaact ctaatgcgcg attaagcaac tgctctggcg
      901 aaacgacttc atttactagc ccaataactt gcgcttcagt gcccgagata aatcgaccag
      961 taaaaattag ctctttagct tttgaacttc cgattaatcg ggctagtcgc tgggtaccgc
     1021 cagctcctgg aataactcca agtaaaacct caggcaatcc aagctgcgca tcagaacttg
     1081 caatcctaaa gtcacaacta agtgcaagtt ctaaaccacc gccaagtgca taacctgaaa
     1141 ccgctgcgat agttggtttt ggaatctttg caactgctgt gaagcaatct tgaagtcctg
     1201 gagtttcgcg agccgcgtct gcgcaattcc agttcaccat ttctttaatg tcggcacctg
     1261 ccgcaaaaga tttttcgccg ccataaatta cgactgttct gatgtctgga ttagtcgaga
     1321 cttcattact tattaatctg atttcttctt gcatcgcgcg gttcagaaca ttcatctttg
     1381 gtcgatccag ccgaatcaaa ccaacagaac catggtgctc taaagaaata aa
//
LOCUS       DBNHVN010000019         1772 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_301174, whole genome shotgun sequence.
ACCESSION   DBNHVN010000019 DBNHVN010000000
VERSION     DBNHVN010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_301174"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..674)
                     /locus_tag="AC16Y1_00350"
     CDS             complement(<1..674)
                     /locus_tag="AC16Y1_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR5830949.1"
                     /translation="MRRFGVLAFSLALITSAFTAPVVAQESTPTEFVDSIIVKYEPGI
                     PVTDSSGDPNGTDAVNTDRNLEVGQNLGLGYRTIEFNEPVPVSEAEKIAAALTGTAEV
                     AIAEPNYEFSVTTEFDPASVSTITQSLSGNFNWGLDRIDQVNLPLDRTYKYPSYQTAP
                     TVYVVDSGIRATHSEFQTNGVSRVLAGFSAFPSPDQSTDDCQGHGTHVAGIIGGNATG
                     VLKNVNL"
     gene            890..>1772
                     /locus_tag="AC16Y1_00355"
     CDS             890..>1772
                     /locus_tag="AC16Y1_00355"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR5830950.1"
                     /translation="MPRPILIDTDPGIDDAVAIALALAEPDVNVIGITTVGGNTGLAN
                     TTRNVLRLLEFLGRTDIPVASGFGEPYKRIAKAPAAEGVHGSDGLGGSDLPDPVTKVV
                     STDSTSFLIERIKGSAEPVTLVALGPLTNIAHLVDADPEIVKHIERFVIMGGAARTGN
                     VTPVAEFNIWHDPEAAKRVFNSGLEVVMVGLDATHSVKTYETLWDWLGKAGNFGNAFK
                     QMLAHYTDFYSAFYGERMTHQHDALAIAEAIWPGTMKLQSAFTDVECESLLTEGMTVV
                     DVNMRQGQKPNSQVAWGL"
BASE COUNT          472 a          371 c          440 g          489 t
ORIGIN      
        1 agattcacgt tttttaaaac accagttgcg ttaccgccaa taattcccgc tacgtgtgtg
       61 ccgtggccct ggcaatcgtc ggttgattga tcgggtgatg gaaatgcaga gaatccggcc
      121 aagactcttg aaactccgtt tgtttggaat tccgaatgcg ttgcccgaat tcctgaatcg
      181 actacgtaga cagtcggagc tgtttggtag ctcggatatt tataggtgcg atcgagcggg
      241 agattcactt gatcaattcg atccaatccc cagttgaagt tgccacttaa tgattgggta
      301 atcgtggaga ctgaggctgg atcaaactca gtggttactg aaaattcgta gttaggctca
      361 gcaatggcaa cctcagcggt gccagtgagt gcggcagcga ttttttctgc ttcgctaacg
      421 gggacaggtt cattaaattc aatagttcgg taaccaagtc caaggttttg cccaacttca
      481 aggtttcgat ctgtatttac cgcatcggtg ccgtttggat ctcctgatga atcagtgacc
      541 ggaatacctg gttcgtattt gacaataatc gagtcaacaa attctgtcgg tgttgattcc
      601 tgggccacca cgggcgccgt gaatgctgat gtaatgaggg caagagaaaa tgccaatact
      661 ccaaagcgtc gcatgctgtt gcctgctccc gagaatttgc tcaattttta cctaaccgag
      721 aattgctgaa accattcctg tagatgattt tacccgagtt tcagaagtcg caacttggct
      781 caatactgaa ggatttatag ccagccccga ggtgtgattc agttaactgg ggtggcaacc
      841 tattgattag aaacccaagt tcaaaatatc ttgtctaagt tgaggaaata tgcctaggcc
      901 aattttgatt gataccgatc caggtattga cgatgcagtc gcgattgctt tagcactcgc
      961 cgaacctgac gtgaacgtaa ttggaatcac aactgttggc ggcaatactg gacttgctaa
     1021 cacaacgcga aatgtgcttc ggttactcga atttttaggc cgcaccgata ttccagtcgc
     1081 atcaggattt ggtgaaccat acaaacgtat tgctaaagct ccagccgcag aaggtgtgca
     1141 cggtagtgat ggactgggcg gaagtgattt acctgatcct gttacgaaag tagtgtccac
     1201 cgattcgaca agttttttaa ttgaaagaat taaaggcagt gcagagccgg tcacacttgt
     1261 tgcattgggc ccacttacca atatcgccca tcttgtcgat gctgatcctg aaattgttaa
     1321 acacattgaa cgatttgtga ttatgggtgg cgcagcacgt actgggaatg taactccagt
     1381 tgcagaattt aatatttggc acgaccccga agcagccaag cgcgtattta attcaggact
     1441 tgaagttgta atggtgggac tagatgcaac gcacagcgtt aaaacttatg aaactctttg
     1501 ggattggtta ggcaaagctg gaaacttcgg aaatgccttc aaacagatgt tggcacatta
     1561 caccgatttt tattctgcat tttatggcga acgaatgact catcagcatg atgccctggc
     1621 tatagctgaa gcaatttggc caggaactat gaaattacaa tctgcgttca ctgatgttga
     1681 gtgtgaaagt ttgttaactg agggcatgac ggtggttgat gtaaatatgc gacaaggaca
     1741 aaaacctaat tcgcaggttg cgtggggact ag
//
LOCUS       DBNHVN010000020         2066 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_895314, whole genome shotgun sequence.
ACCESSION   DBNHVN010000020 DBNHVN010000000
VERSION     DBNHVN010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_895314"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="AC16Y1_00360"
     CDS             complement(<1..313)
                     /locus_tag="AC16Y1_00360"
                     /inference="COORDINATES: protein motif:HMM:NF047172.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR5830951.1"
                     /translation="MTKWLSKDQQVHWRSWITASSLLPELLSRELQEEFGLTITDYEI
                     LVRLSEASNKQMRMSELAQKTMLSRSRLSHQIDRLEDRGLVNRRSCEDDRRGQNCVMT
                     NK"
     gene            complement(357..1112)
                     /locus_tag="AC16Y1_00365"
     CDS             complement(357..1112)
                     /locus_tag="AC16Y1_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR5830952.1"
                     /translation="MAAHSFTAEVVTLSVDIGGSGLKCALLNATGEMISERLRIDTPY
                     PCPPERLISALVELTKPLTGYHRMSVGFPGLIREGKVVNISSLSKSTLEGEVDQDLVT
                     AWHNFDLAQGLADAFKVPIRVANDADVQGSAVIKGHGLEFVMTLGTGCGAALFYEGKL
                     LPHLELGHAPFRKGQSFEDQIGNAARKEVGKERWIKRVRKAITAYDSFLFFDHIYIGG
                     GNARHLLEEDFGPNVTIVENTAGILGGIKIWET"
     gene            complement(1114..1974)
                     /gene="map"
                     /locus_tag="AC16Y1_00370"
     CDS             complement(1114..1974)
                     /gene="map"
                     /locus_tag="AC16Y1_00370"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018134692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR5830953.1"
                     /translation="MTTKVGTLTPGIISPTRIVPTEIARPEYVGKKAPSKSKDSDIRD
                     NETIEKMRIAGRIAAGALIEVGKHVAPGITTDELDRIGHEFVCDHGAYPSTLGYRGFP
                     KSLCASLNEVICHGIPDSTVIQDGDICNIDITAYIHGVHGDTNATFLAGEVDEESRLL
                     VERTQESLTRAIAAVQPGRPINVIGRVIESYAKRFGYGVVRDFTGHGVGTSFHTGLIV
                     PHYDTDEFATLMEVGMTFTIEPMLTLGTHHWDMWKDNWTVVTADRKRSAQFEHTLVVT
                     PSGAEILTKV"
BASE COUNT          550 a          494 c          449 g          573 t
ORIGIN      
        1 ctttattggt catgacgcaa ttctgaccac gcctgtcatc ctcacaacta cgtcgattaa
       61 ctaggccgcg atcctctaag cgatcaattt gatgggaaag acggcttctg gaaagcatcg
      121 ttttttgagc aagctcactc attcgcattt gcttattgga agcctcagaa agtcgaacta
      181 aaatttcgta gtccgtaata gttagtccaa attcttcttg cagttcccga cttaacaact
      241 caggaagcaa actagaggcc gtaatccaag aacgccaatg cacctgctga tctttgctga
      301 gccacttagt catagttgaa ttttagacga ttaattgaaa attgaactaa tctggcttaa
      361 gtttcccaga tttttattcc gcctaaaatt ccagcggtgt tttccacgat agttacattc
      421 ggtccaaagt cttcttcaag taggtgacgt gcattgccac cgccaatgta aatgtggtca
      481 aagaataaaa acgagtcata agcagtgatt gcttttctaa cccgcttaat ccagcgctct
      541 ttgccaactt cttttcgagc tgcattcccg atctgatcct caaaactttg gcctttgcga
      601 aatggcgcat gccctagttc tagatggggt agcaatttgc cttcataaaa aagtgcagcg
      661 ccacagcctg tgccgagggt catgacgaac tcaagtccat gccccttaat taccgcactg
      721 ccttggacgt cggcgtcatt agcaactctg atcggaactt tgaaggcatc agccaaaccc
      781 tgggctaaat caaagttatg ccaagcagtt accagatcct gatcgacttc accctcaaga
      841 gtgcttttgc taagcgaaga tatgtttact actttgcctt cgcgaatgag accggggaag
      901 ccaacagaca ttcggtggta gccagtcagt ggtttggtca attcgacgag tgcactaatt
      961 aatcgctcag gggggcaagg gtatggagta tcaattcgaa gacgttccga aatcatttca
     1021 cccgtggcat tgagcaatgc gcactttaat ccactgccgc caatgtcaac tgacaaagta
     1081 actacttcag ctgtaaaact gtgcgctgcc atgtcacacc ttagttagaa tttcggcccc
     1141 ggatggagta accaccaacg tatgttcaaa ctgggcgctg cgtttacggt cagcggttac
     1201 tacagtccaa ttatctttcc acatatccca atgatgggta ccaagggtga gcataggctc
     1261 aattgtgaaa gtcataccta cttccataag tgtggcaaat tcatcagtgt catagtgggg
     1321 gacgattagc ccagtatgaa aacttgtccc aacgccatgt ccagtgaaat ctctcactac
     1381 gccgtaacca aatcttttgg cgtaagactc aatcactcga ccaattacat ttattgggcg
     1441 cccaggttga acagctgcaa ttgctcgggt tagagattct tgcgttcgct ctacgagcaa
     1501 ccttgattct tcatcaactt caccagctaa aaatgttgcg ttagtgtcgc cgtgaactcc
     1561 gtgtatgtat gcggttatgt caatgttgca aatgtcgcca tcttggataa ctgtcgaatc
     1621 aggaatcccg tggcaaataa cttcattcaa actggcacat aatgattttg ggaatccgcg
     1681 atagcccagc gttgatgggt aagcaccatg atcacaaaca aattcgtggc caatgcgatc
     1741 taactcatct gtggtgatac ctggcgcaac atgtttgcca acttcgatca gtgcgccagc
     1801 agcgatccgt ccagcgatcc gcattttttc aatagtttcg ttatcgcgaa tatctgagtc
     1861 tttagattta ctaggtgcct ttttccctac gtattcagga cgggcaattt cagttggcac
     1921 aatgcgagtt ggcgaaataa tgcctggtgt taaagtgcca actttggtgg tcataatttt
     1981 ttaagtttag agcaaataca aaagccccag tttgcagcgg gcatttttgt attgggaaat
     2041 acaaaagccc cagtttgcag cgggca
//
LOCUS       DBNHVN010000021         2257 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_78492, whole genome shotgun sequence.
ACCESSION   DBNHVN010000021 DBNHVN010000000
VERSION     DBNHVN010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_78492"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="AC16Y1_00375"
     CDS             <1..519
                     /locus_tag="AC16Y1_00375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003970161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016426 - tRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HIR5830954.1"
                     /translation="ETGLLHSFERREEFAQIAQKNVQAFFGKLPNNWNLHLSDFQEQA
                     SAADPKALPEHSVDRVILDMLAPWECISAVSRILKPGGVVMVYVATTTQMSNYVEQLR
                     ETKEFTDPQAQELIARNWHVEGLAVRPDHKMHGHTGFLVLARRLAVGTLLPPRRIRPA
                     KGAESTATEVKS"
     gene            565..2172
                     /gene="arc"
                     /locus_tag="AC16Y1_00380"
                     /pseudo
     CDS             565..2172
                     /gene="arc"
                     /locus_tag="AC16Y1_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004946292.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
BASE COUNT          729 a          436 c          508 g          584 t
ORIGIN      
        1 gaaactggat tactgcacag ttttgagcgg cgtgaagaat tcgcacaaat tgcacagaaa
       61 aatgttcaag cattttttgg caaattacca aataactgga atttacattt aagtgatttt
      121 caagaacagg cgtctgcggc agatccaaaa gcattacccg agcactcggt tgatcgagtt
      181 atcttagaca tgttagctcc gtgggaatgt atttctgccg tgtccagaat cttgaaaccc
      241 gggggcgtag taatggtcta tgttgcaact acaacacaga tgtccaacta cgtagaacaa
      301 cttcgggaga caaaagaatt caccgatcca caagcccaag aacttattgc acgaaattgg
      361 catgtcgagg gattggcagt tagaccagac cacaaaatgc acggacatac tggattttta
      421 gttctggccc gacggttagc agtcggcaca ttgttacccc ctcgaagaat tcgaccggct
      481 aaaggtgcag aaagtactgc caccgaagta aagtcataag tggaggattc taaattgtct
      541 gaagttactg cccaaaatga tcaaacgcgt gcactgcacg gcaagattga tgaattggca
      601 gaaaacaatc aaaaattggc agctactctt cgtgatgcac gcgaacaaat tatcgggtta
      661 aagaatgaaa tcgaaaagtt aagttcacca ccaaatggtt atgcaacttt tctaggtaat
      721 gcagaaaaag gtctggttaa cgttgcagtt aacggacgaa agatgcgggt tgaaattgcg
      781 ccgagtattg accccaaatc actcaaacct ggtcaagaag taattcttaa tgagtccatg
      841 aatgttgttg tcgcaggaga ttttgaaact catggtgaga tggccacttt ccatgagtta
      901 ctgccagatg gtgaccgagc catcgtgata ggcagaaatt ctgaagagcg aatagttttg
      961 ctggctgaac cgttaaaaaa tgaacagtta aaaacagggg atagcttact ttttgatacc
     1021 cgttctggtt tcgcctttga acttgtaaat aagactgaag ttgaagaact agtccttgaa
     1081 gaagtcccag atgttgatta caccgacatt ggtggtttag gtaaccaaat agagcagatc
     1141 agagatgctg ttgaactgcc attcttacat cgagatttat acaaagaata cgaattaaag
     1201 gcaccaaaag gaattttgct atatggtcca cccggatgtg gcaaaacttt gattgctaag
     1261 gcagtagcta attcccttgc gaaaaaagtt actgaacttc gtggaattgg cgagggccga
     1321 agttatttct tgaacgttaa aggtcctgaa cttctaaata aatatgttgg tgaaactgag
     1381 cgtcaaatcc gattaatttt ccaacgtgct cgcgagaaag caaatgaagg aactcccgta
     1441 attgtcttct ttgatgaaat ggatagcttg tttagaacac gcggtaccgg cgtctcaagt
     1501 gatgttgaga atacgatcgt tccccagtta ttggctgaga ttgatggtgt tgaatcactt
     1561 gaaaatgtga ttgtgattgg tgcatcaaac cgtgaagaca tgatcgaccc agcaattctt
     1621 cgaccgggtc gtcttgatgt caaaattaaa attgaacgac ctaatgccca agctgccaag
     1681 gaaatcttca aaatttattt aactgcagat ttaccaattc acgttagcga atcaactaag
     1741 tatggaaata cgcaagacgc tgtaacgagc atgatcgatc aattagttga gcaaatgttt
     1801 agcacaactg cagcgaacga atttctggaa gtcacttacg caaatggcga aaaggaagtt
     1861 ctgtatttta aggacttcag tagtggtgcc atgattgaaa acatcgttgc acgggctaaa
     1921 aaagatgcaa ttaaaaacta tctggccaca aacgaatcag ggattaaatt agaacattta
     1981 ttaaaagcag ctcgagaaga attccgcgag aatgaagacc tgccaaatac tactaatcca
     2041 gatgattggg ctcgaatttc cggcaagaag ggtgaacgga ttgtcttcat gcgaactttg
     2101 attcagaatg aacagggact tgaatctgga cgcacgattg atacaactaa cgtaacttcg
     2161 cattttctct aaatgatgta aacatgtctg tccatagaat tatgggaatc gaatccgaat
     2221 tcggaatcgc cagtccaaca aatccaaccg caaaccc
//
LOCUS       DBNHVN010000022         2894 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_78566, whole genome shotgun sequence.
ACCESSION   DBNHVN010000022 DBNHVN010000000
VERSION     DBNHVN010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_78566"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="AC16Y1_00385"
     CDS             complement(<1..274)
                     /locus_tag="AC16Y1_00385"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5830955.1"
                     /translation="MMRSLGAYVHIPFCASRCGYCDFNTYTASELSRDGSIINTTNYV
                     DRVVLEIDRQHKEISHNDRPLETIFFGGGTPTLLGSRELVRALKYLE"
     gene            complement(274..909)
                     /locus_tag="AC16Y1_00390"
     CDS             complement(274..909)
                     /locus_tag="AC16Y1_00390"
                     /inference="COORDINATES: protein motif:HMM:NF015438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR5830956.1"
                     /translation="MSAKVISLNVVHDVIPDVGGTVGITAIDKRPVTGHRQVTTAGIS
                     GDQRSDLKHHGSPTQAVYAYAKEDYAWWQKELGFNLSFGQFGENLTTENFDLTNLVIG
                     TKIKIGDTQLRATAPRIPCGTFARWMNLDQWVKRFTDGERVGSYFEVLASGEISAGDE
                     IEVISAPDHAVTVLDYFRVHTGDRDQVRVEKCVNCVDILPEVREKFSKFIN"
     gene            complement(912..2765)
                     /gene="lepA"
                     /locus_tag="AC16Y1_00395"
     CDS             complement(912..2765)
                     /gene="lepA"
                     /locus_tag="AC16Y1_00395"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR5830957.1"
                     /translation="MATSAPAPGATSPEIIRNFCIIAHIDHGKSTLADRMLQITGVVD
                     ARNMRAQYLDRMDIERERGITIKSQAVRLPFKAADGVDYVLNLIDTPGHVDFTYEVSR
                     SLAACEGAILLVDAAQGIEAQTLANLYLALENNLTIIPVLNKIDLPAAQPEKYAAEIA
                     HIIGCDPDEVLKVSAKTGAGVEDLLNVVVQRVPAPVGDANAPARALIFDSVYDTYRGV
                     VTYVRVIDGRIPQREKIKMMSTNEQHEILEVGVISPEPIASKGIGVGEVGYLITGVKD
                     VRQSKVGDTVTLAANPAKVALGGYREPKPMVFSGLYPLDGSDFPELREALDKLKLNDA
                     ALVYEPESSVALGFGFRCGFLGLLHLEIVRERLEREFNLDLISTAPNVIYQVKLDNGK
                     ELTVTNPSEFPTGKISEVHEPIVRATILTPNEFVGAIMELCQTRRGTLNGMDYLSEDR
                     VEMRYTLPLAEIVFDFFDQLKSRTRGYASLDYETTGDQSADLVKVDILLHGDPVDAFS
                     AIVHREKAYAYGVLMAAKLKELIPRQQFEVPIQAAIGAKIIARETIRAMRKDVLAKCY
                     GGDISRKRKLLEKQKEGKKRMKMVGRVEVPQEAFIAALSTDNNAAKAKAKD"
BASE COUNT          773 a          670 c          584 g          867 t
ORIGIN      
        1 gttccaaata tttcaaagct cggactaatt ccctggaccc aagcagtgtt ggggtgccac
       61 cgccaaaaaa tattgtttct agtggacgat cattatgcga aatttcttta tgctgacgat
      121 cgatttctag gactactcga tcaacatagt tggttgtatt gataattgaa ccatctcggg
      181 ataattcact ggcggtgtaa gtattgaagt cacaatatcc acacctagaa gcgcagaatg
      241 ggatgtgcac gtaggcacca agacttctca tcattagtta atgaactttg aaaacttttc
      301 tcggacttcc ggcaaaatat cgacacaatt tacgcatttt tcaactcgaa cttgatcgcg
      361 atctccagtg tgaactcgga aataatcaag aacagtgacc gcgtgatctg gagctgaaat
      421 tacttcaatt tcgtcacctg cactgatttc tccggaagcc aagacttcaa agtaggaacc
      481 tacgcgttca ccgtcagtaa accttttaac ccattgatct aaattcatcc aacgtgcgaa
      541 tgtgccgcac ggaattcgtg gtgcggtagc acgtaattga gtatctccaa tttttatttt
      601 tgtaccgata actaaattgg ttaaatcaaa attttcagtg gtcagatttt caccaaactg
      661 gccaaaactt aggttaaaac caagttcttt ttgccaccac gcataatcct cttttgcgta
      721 tgcataaaca gcttgggttg gcgagccatg atgctttaaa tctgagcgtt gatcaccaga
      781 aataccagct gtcgttactt ggcggtgccc agtaactgga cgtttatcaa ttgccgtaat
      841 tccgactgtt ccaccgacat ccggaataac gtcgtgcaca acattaagtg aaataacttt
      901 ggcactcacg gttagtcctt ggcttttgct tttgctgcat tgttatcagt tgataatgcc
      961 gctatgaaag cttcttgcgg cacttcgact cttcccacca tcttcatgcg cttcttgcct
     1021 tctttttgtt tttcaagaag tttgcgctta cgtgagatat cgccaccgta acattttgcc
     1081 aatacatctt ttcgcattgc ccgaatagtt tcacgagcga taatttttgc gccgattgcc
     1141 gcctgaattg ggacctcgaa ttgctgacgt ggaattaact cttttaactt ggccgccatt
     1201 agtacaccgt atgcatatgc cttttcgcga tgcacgatgg cactaaaagc atcgactgga
     1261 tcaccatgta acaaaatatc gactttaact aaatctgcag actgatcgcc agttgtttca
     1321 taatcgagtg aggcatagcc acgggttcgg gatttaagtt gatcaaagaa atcaaagact
     1381 atttcggcca gcggcagggt gtaacgcatc tcgactcgat cttcggacaa atagtccatc
     1441 ccatttaaag tgcctcgtct agtttggcaa agttccatga tggctccgac aaactcattt
     1501 ggggtcaaaa ttgtggcccg cacaattggt tcgtgtactt cagagatttt tccagtggga
     1561 aattcacttg gatttgtaac cgttaactct ttgccattat ctagtttgac ttggtaaata
     1621 acgtttggcg cagttgaaat aaggtctaag ttaaattctc gctctaatcg ctcacgcacg
     1681 atttctagat gtagcaatcc taaaaatcca caacggaaac caaagccaag tgcaactgag
     1741 gattcaggtt cataaactaa agcagcatca ttaagtttta atttatctag tgcttcacgt
     1801 agttctggaa aatctgatcc atcaagagga tacaaaccag aaaacaccat cggctttggt
     1861 tctcgatatc cacctagcgc taccttggcc gggttagctg caagtgttac cgtgtcacca
     1921 actttggatt gccgaacgtc tttcacgcca gtgattaaat atcctacttc gcctacacca
     1981 atgccctttg atgcaattgg ctctggtgaa attaccccaa cttctaaaat ttcatgctgc
     2041 tcgttggtac tcatcatttt aattttttcc cgttgtggga tacgaccgtc gattacgcga
     2101 acataagtca ccacgccgcg ataagtgtca tacacagagt caaaaattaa tgcgcgtgct
     2161 ggcgcatttg catcaccaac tggtgcggga actctctgca caactacgtt taataaatct
     2221 tcaacgccgg ctcctgtttt ggcgctaact ttaagtactt catcaggatc acagccaata
     2281 atgtgggcaa tttcagctgc atacttttca ggttgtgctg ccggtaaatc aattttgttc
     2341 agcactggaa taattgttaa attattttcc agtgctaaat aaagatttgc taaagtttgc
     2401 gcttcgatgc cttgagcagc atcgaccaat aaaattgcac cttcacatgc cgccaagctc
     2461 cgactgacct cataggtgaa atcgacgtgc ccaggcgtgt caattaaatt caatacataa
     2521 tcaacgccat cggcggcttt aaatggcagg cgcactgcct ggcttttaat ggtgatgcca
     2581 cgctcacgtt cgatatccat tcgatccaga tattgcgctc gcatattcct tgcatcaaca
     2641 acaccggtga tttgcagcat tcgatcggcc agcgttgatt tgccatgatc aatgtgcgca
     2701 ataatgcaga aatttcgaat tatttctggc gacgttgccc ctggagcggg tgcagatgta
     2761 gccatgtaaa tccttttagt taatgcttat ttttaaggga attgccctgc cattttgaca
     2821 tagtttggct taatttgagc aattgcctat ttcacaggta atcttgtggc ttgcacctgc
     2881 tcattagtta agta
//
LOCUS       DBNHVN010000023         2573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1267853, whole genome shotgun sequence.
ACCESSION   DBNHVN010000023 DBNHVN010000000
VERSION     DBNHVN010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1267853"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="AC16Y1_00400"
     CDS             <1..717
                     /locus_tag="AC16Y1_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024748.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR5830958.1"
                     /translation="NLTAWTSAHSPGTFNATVQDVLNNCTYAIGNAEATFPMASTGKV
                     IIATGVLEKVARGELDYASVEADMRSMITVSDNGAADRLFALIGKAPTFVDIETRYGL
                     TQTKTDRGWGTTLTNSADQVKLLDQIIGISSSPLPDIQRQILRDLMKNVVPDQAWGAG
                     TNIPAGWSVAVKNGWYQTVPGDIPPVGLWRVNTVGMTWDESGTPRWIFAAYSNEWPTQ
                     SAGVSAWNDLAALITANLAN"
     gene            complement(718..1302)
                     /locus_tag="AC16Y1_00405"
     CDS             complement(718..1302)
                     /locus_tag="AC16Y1_00405"
                     /inference="COORDINATES: protein motif:HMM:NF014600.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR5830959.1"
                     /translation="MKKRIALLCSIASLLVLISSPVNAKDRIVNVCIHKKTGYIRQSA
                     IATCLPAELPVGAKALTAGNVRPKQMNSFFANRIKIAQLMGQKEGYELRITSGWRSVE
                     YQKKLFNNAIKENGSVAEASKWVLPPEYSMHPWGLAVDINYKIGRPEGAKWLEDNGYK
                     FGLCRRYENEWWHFEPLVAPGDKCPALEEYPVAQ"
     gene            complement(1386..>2573)
                     /locus_tag="AC16Y1_00410"
     CDS             complement(1386..>2573)
                     /locus_tag="AC16Y1_00410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5830960.1"
                     /translation="TAYQIALEGTRVTLVDSGEPKYGTSNQNAGWIAPSHVIPFAAPG
                     MVQMGFEQLLKRTGAFGFSPAAGPLLASWTYKFVRSCTQEHVDKSVPALTELLDTSIA
                     GFEKLISSHGLTKAPEALWYIFSTDKAAEHAHHEVELMSKYGVGAKEVDIATARAKEP
                     ILKETAKAIVEFNTDFGVDPKLLIEILTKLCIEHGVEIRNNESVKKIASTKQNVSITT
                     DKDSWTCEYLVVAAGVWSRELVKTLGVNLPIMPAKGHSITIPDLPNQPKRPFLLAEQR
                     IAGNSLDWGLRLSTGYTLTSPNDRSVEMKAVEKLIQTASKVLDIPTSTANVNPWSGLR
                     PSSPDGLPYIGPLSKAPRVIMATGHGMLGTMMALGTSVLVADMVAGRPVSREVLKFSP
                     SRP"
BASE COUNT          710 a          560 c          552 g          751 t
ORIGIN      
        1 aacttaaccg catggacatc tgcgcacagt ccaggaacat ttaatgcgac tgtgcaagat
       61 gttttaaata actgcactta cgcaattggt aatgccgaag caacatttcc aatggcatcg
      121 acagggaaag taattattgc aactggtgtc ctagaaaaag tcgctcgagg cgaacttgat
      181 tacgcatcag ttgaagctga catgcgctcg atgattactg tgtctgataa tggcgccgcc
      241 gatcgattat ttgcgttgat tgggaaagca ccgacttttg tagatatcga aactagatat
      301 gggttgactc aaactaaaac tgatcgcggc tggggaacaa cactgactaa ctctgctgat
      361 caagttaagt tactcgatca aataattggg atttcatcga gccctcttcc tgatattcag
      421 cgccagattt tgcgggactt aatgaaaaat gttgtgccag atcaagcttg gggagctggc
      481 accaatattc ctgctggttg gagtgtggcg gttaaaaatg gttggtacca aactgttccg
      541 ggtgacattc caccagttgg attatggcgc gttaatactg tggggatgac ttgggacgaa
      601 tcaggtacac cacgatggat ttttgcggct tattcaaatg agtggccaac ccaaagcgcc
      661 ggagtttcgg cctggaacga tttggcagca ttgatcactg caaaccttgc taattaatta
      721 ttgagcaact gggtattctt caagcgctgg acatttgtcg cccggcgcaa ctagtggctc
      781 aaagtgccac cattcatttt cataccttcg gcataggcca aatttatatc cgttatcttc
      841 gagccactta gcaccttctg ggcgacctat tttgtaattt atatcaaccg ctaaacccca
      901 aggatgcatt gaatactcag gcggcaaaac ccatttactt gcttctgcta cagaaccatt
      961 ttctttgatc gcattattaa aaagcttttt ctgatattca acagaacgcc acccactagt
     1021 gattctgagt tcgtaaccct ctttttgtcc catcaattgc gcaattttta tgcgattagc
     1081 aaaaaaacta ttcatttgtt ttggtcgaac attgccagca gttaatgctt ttgcaccaac
     1141 tggtaactca gccggaagac aagttgcaat tgctgactga cgaatgtagc cagttttttt
     1201 gtggatacaa acattaacaa ttcgatcttt ggcatttacc ggtgagctaa tcaggacaag
     1261 caggcttgca attgaacata acaacgcaat ccgctttttc attacgcaac ttcctatcta
     1321 cattcagcgt aattaaatga actgaaataa cgaaattgca gttgagaatg cagaagtaac
     1381 ctcagttaag gacgcgatgg cgagaacttt aaaacttcgc gcgagacagg tctaccagcc
     1441 accatgtcag caaccaatac tgatgtgcca agtgccatca ttgtgccgag catgccatga
     1501 cctgttgcca tgatcacccg aggagcttta ctaagcgggc caatgtatgg caatccatct
     1561 ggacttgatg gacgtaaacc tgaccatggg ttgacattcg cagttgaagt tggaatatct
     1621 aaaactttgc tcgcagtttg gattaacttt tcgactgctt tcatttccac tgatcgatca
     1681 tttggtgaag tcaaggtgta accagtactt aagcgcaaac cccaatcaag ggagtttcca
     1741 gcaattcttt gttcggcgag taagaatggt cgtttcggct gattgggtaa atcaggaatt
     1801 gttattgagt gaccttttgc tggcattatt ggcaaattaa ccccaagcgt cttaactaac
     1861 tcacgggacc aaacaccggc agcaacaacc aaatattcac aagtccacga atctttatcg
     1921 gtagtgattg aaacattctg tttcgttgat gcaatttttt tcactgactc gttatttcta
     1981 atctctacac catgttcgat acagagtttg gttaaaattt cgatcagtaa ttttggatct
     2041 acgccgaagt cagtattgaa ctcaactata gcttttgcag tttcttttaa aattggttct
     2101 tttgcacggg cggttgcaat atctacttct tttgccccaa ctccgtattt gctcattaac
     2161 tcaacttcat gatgagcatg ttcggccgct ttatctgttg aaaatatgta ccaaagtgct
     2221 tcgggtgctt tggttaaacc atgacttgaa attaattttt caaatcccgc aatgctggta
     2281 tcaagtaatt cagttagcgc gggaactgat ttgtctacat gctcttgtgt acaagatcgc
     2341 acaaatttat aagtccatga ggcaagtagt ggaccagcag caggcgagaa gccaaatgca
     2401 ccagttcgct taagaagttg ctcgaatccc atttgcacca tcccaggggc agcaaaggga
     2461 attacatgtg aaggtgcaat ccagccagca ttttgatttg aagttccata ttttggttcg
     2521 ccagagtcaa ctaaggtgac gcgcgttcct tcaagtgcaa tttgatatgc agt
//
LOCUS       DBNHVN010000024         1607 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1862236, whole genome shotgun sequence.
ACCESSION   DBNHVN010000024 DBNHVN010000000
VERSION     DBNHVN010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1862236"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="AC16Y1_00415"
     CDS             <1..335
                     /locus_tag="AC16Y1_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005445336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR5830961.1"
                     /translation="EIGGGSIRIHQSDVQEKVFKILGLTEQEAKDKFGFLLEAFSYGP
                     PPHGGIAFGWDRICMLLAGAQSLREVIAFPKTGAGYDPLTSAPTPITEQQRLEAGIDV
                     DPFAKTDS"
     gene            339..>1607
                     /locus_tag="AC16Y1_00420"
     CDS             339..>1607
                     /locus_tag="AC16Y1_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016455957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR5830962.1"
                     /translation="MTSLFSGDSIATPLAVRMRPKNLTEFEGQNQVLTEGGPLHRLLN
                     FNQKQSATSIIMWGPPGTGKTTLAQLIANNGKAKFVELSAVTAGVKEVREVIGQAAEQ
                     KSLYGLQTVLFIDEVHRFNKAQQDALLPGVENGSITLIAATTENPSFSVISPLLSRSL
                     LITLTSLTDENVAKLIHRALVDERGLANKYEIESEAEALIIRMAGGDARRALTILEAA
                     SQIASVAKTNLISVDAVSKSVDQHVVRYDRDGDQHYDVISAFIKSVRGSDVNAALHYL
                     ARMIEAGEDPRFIARRLIILASEDIGLADPNALVNAVACAQSVALIGFPEARIPLAQT
                     TISLCLAPKSNSAYLAIDRAIADVRAGKIGEVPSHLRDSHYARAGSMGHGVGYLYPHD
                     LAEGVAAQSYLPVELEAANYYEPTHRGHESN"
BASE COUNT          454 a          336 c          390 g          427 t
ORIGIN      
        1 atgagattgg tggcggatcc atccgaatcc atcaaagtga tgttcaagaa aaagttttca
       61 aaatacttgg cctaactgaa caagaagcaa aggacaaatt tggtttcttg cttgaagcgt
      121 tcagttatgg acctccaccg catggtggaa tagcatttgg ttgggatcga atttgcatgt
      181 tacttgctgg agcgcaaagc cttcgcgaag taatagcttt tccgaaaact ggtgctggat
      241 atgatccgct aaccagtgcg cctactccaa tcacggagca acaaaggtta gaagcgggta
      301 tagacgttga tccattcgca aaaactgaca gttgattgat gacctcactt ttttcgggtg
      361 acagtatcgc tacgccgtta gcggtaagaa tgcgaccgaa gaatttaacc gaattcgagg
      421 gacaaaacca agtacttact gaaggtggac cacttcatcg gctgcttaat tttaatcaaa
      481 agcaaagtgc aacttcgata attatgtggg ggcccccggg aaccggtaag actaccttgg
      541 ctcaattgat cgcaaataac ggtaaagcca aatttgtcga gttatcggcc gttactgctg
      601 gggtaaagga agtcagagaa gttattggcc aggcggctga gcaaaaatcg ctttatggat
      661 tacaaactgt actttttata gatgaagtac ataggttcaa caaggcacag caagatgctt
      721 tgttacctgg tgttgagaat ggttcgataa cactaatagc cgccaccacc gaaaacccat
      781 cgttcagtgt tatttctcca cttttatcca gatcattatt aatcacgctg acgagtctga
      841 ctgatgagaa tgtcgccaaa ttaatccatc gtgctttagt cgatgagcgg gggttagcca
      901 acaaatacga aattgaaagt gaagcagagg cacttattat tcggatggct ggtggtgacg
      961 ccagaagagc actcacaatt ttagaggcag ctagccaaat tgcttctgtg gctaaaacca
     1021 atttgatttc tgttgacgca gtcagcaaat cagttgacca gcacgtagtt cgttatgacc
     1081 gagatggcga tcaacattat gacgtgatta gtgcatttat taaaagtgtg cgaggttcag
     1141 atgtaaatgc cgcacttcat tatttagcca ggatgatcga ggctggtgag gatcctcgat
     1201 ttattgcccg aagattaatt atcttggcaa gtgaagatat cggattagct gatccaaatg
     1261 cattggttaa tgccgtcgct tgcgcacaaa gtgttgcact cattggattt ccggaagcgc
     1321 gtattccgct ggcccaaacc acaatttcat tatgtcttgc acctaaatca aactctgcct
     1381 acttggccat agatcgggct atagctgacg tacgagctgg aaaaattggg gaagttccta
     1441 gtcatttgcg cgatagtcat tacgctcgcg ctggctctat gggccacggt gttggttatc
     1501 tctacccgca tgatcttgcg gaaggtgtgg cagcgcagtc atatctacca gtggaacttg
     1561 aagcagcgaa ctactacgaa ccaacgcatc gaggtcatga gtcgaat
//
LOCUS       DBNHVN010000025         2150 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1416272, whole genome shotgun sequence.
ACCESSION   DBNHVN010000025 DBNHVN010000000
VERSION     DBNHVN010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1416272"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="AC16Y1_00425"
     CDS             <1..560
                     /locus_tag="AC16Y1_00425"
                     /inference="COORDINATES: protein motif:HMM:NF044969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HIR5830963.1"
                     /translation="KFAKDVSRLSTRLPELLRGIEKTRYEREVQKYLGLGAPETLARQ
                     VAGLLDVFSLLDVVEIANRHKADPDRVVELYFALSERYDVDRLLHHITQLPRDDRWNA
                     YARSALRSDLYVALAGLTSRVTQATQQSESVDQRIENWESKFAEGVGRTRLTLNEIAA
                     SENTDLATLSVALRAIRTLVGQGAS"
     gene            590..1225
                     /locus_tag="AC16Y1_00430"
     CDS             590..1225
                     /locus_tag="AC16Y1_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR5830964.1"
                     /translation="MFDLVLAAALAGQVTDWLDRFGPFLFYTIVFGLVFAGTGLFIGA
                     FIPFITGDSLIFAAGVLAASSDRIDIQVLAIGIGIAAFMGDQVGFVLGRHYGRPYLDK
                     REGPKIKLAIEKSEQFYEKLGWWAVVVARFIPWARVLVPAIAGIARMNYYKFLGANLV
                     GALVWGVALPLAGYYAVTIPAVRTVTYAIATFFIVGSIVAGIRSWRANRTH"
     gene            1351..1890
                     /locus_tag="AC16Y1_00435"
     CDS             1351..1890
                     /locus_tag="AC16Y1_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019136240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR5830965.1"
                     /translation="MTDISALKGTWVFDPSHSRLGFEARHAVVTKVRGHFTNFDGTVV
                     IGDDLAGSSVKVNAKLDSIDTNSADRDAHLKSADFFDVENTNDLSFVSTSIKADGDDF
                     VVKGNLTIKGVTKEVELEVEATGTATDPFGNLRAGFEGKGEISRKDFGLTWNVALEAG
                     GVLVSDKIKLQLDISAIKQ"
BASE COUNT          565 a          439 c          509 g          637 t
ORIGIN      
        1 ataagttcgc caaagatgtt tctaggttaa gtaccagact ccccgaatta ttgcgcggta
       61 ttgaaaaaac tcgctacgaa agggaagtgc aaaagtacct gggcttagga gctccggaaa
      121 ccttagcacg acaagttgct ggattattgg atgtattttc cctactagat gtagtcgaaa
      181 ttgctaaccg acataaggct gatccagatc gagttgttga actttatttt gcgttgagcg
      241 aacgttatga cgtggatcga ttacttcacc acatcacaca gttgccacgg gatgatcgtt
      301 ggaatgcata cgcgcgatct gccttgcgca gtgatttgta tgttgctctt gctggactta
      361 cttctcgtgt gacgcaggcc acacaacagt ccgagtctgt agatcaacgg attgaaaact
      421 gggaaagtaa atttgctgaa ggtgttggtc gaactcgact aactttaaat gaaattgcag
      481 ctagtgagaa tactgaccta gcaacattat ctgttgcact tcgcgcaatc agaactcttg
      541 tcggacaggg tgcaagttag tctgcaaaaa aaccaattag actgagtaag tgtttgattt
      601 ggtattagca gcagcgctcg ctggacaagt tacagactgg cttgatcgct ttggtccatt
      661 tcttttctac acaattgttt ttggtttagt ctttgctggc actggattat ttattggtgc
      721 ctttattccg ttcattactg gggattcgct tatttttgct gcaggtgtgc tggcggccag
      781 ttcggatcgt atagatattc aagttttggc aatcggaatt ggtattgccg catttatggg
      841 cgatcaggtc ggcttcgttt taggcagaca ttatggccga ccttatttag acaagcgtga
      901 aggcccaaaa attaagcttg ccatcgaaaa gtccgagcaa ttttatgaaa aattaggctg
      961 gtgggccgtg gtcgttgcac gtttcattcc atgggctcga gtcttggtac cagcaattgc
     1021 tggaattgcg aggatgaatt actacaagtt tttaggggcc aacttggttg gtgcactggt
     1081 ttggggtgtg gcgctgccac ttgctggcta ttacgcagta actattcccg cggtacgcac
     1141 cgttacctat gcgattgcta ctttctttat tgtgggttca atcgttgctg gcatacgcag
     1201 ctggcgagcc aaccgtaccc attaatccct ttcgcaaaat acatctcaac atcggcagac
     1261 cttgcatttt ccggagcaaa tcttgatttg acagatagtt gaatattaaa ctaaaatagt
     1321 tcaaatttca actatctata aggagtcgca atgactgata tttcagccct aaagggaacc
     1381 tgggtttttg acccgtcaca tagccgtctt ggtttcgaag cacgtcacgc agtcgtcaca
     1441 aaggtgcgtg gacatttcac caattttgat ggcaccgtag taattggtga tgacctagct
     1501 gggtcatcag taaaagtaaa tgccaagtta gattcaattg atactaactc ggccgatcgc
     1561 gacgcccacc ttaagagtgc tgatttcttc gatgtagaaa acaccaatga cttgtcattt
     1621 gtttcaacca gcatcaaggc agatggtgat gactttgtag tcaagggaaa cctaacaatt
     1681 aaaggtgtaa ccaaagaagt tgaactagaa gttgaagcaa ctggaacagc tacggatcca
     1741 tttggcaacc tacgtgccgg ctttgaaggt aaaggcgaaa ttagtcgcaa agactttgga
     1801 cttacttgga acgtcgccct tgaagcaggt ggcgtactag ttagcgacaa gatcaaactt
     1861 caattagata tctcagcaat caagcagtaa ttatttagtt cattcctctc cgaaacaaaa
     1921 agggccatcc tcgggtggcc ctttttgttt tagttgtcaa ttggagtaca aaaattgtgc
     1981 ataactttag ataaattgta attattagtc cacttatccc cagatttggt ttttttagtt
     2041 tcatttgcat cgaacagact aactttccac taggcgcagt tcttggccgg cgccgctggg
     2101 ggagttaatg tctgatgtca ttgaagattt agttgagcag gttcggcatc
//
LOCUS       DBNHVN010000026         1083 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_2011061, whole genome shotgun sequence.
ACCESSION   DBNHVN010000026 DBNHVN010000000
VERSION     DBNHVN010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_2011061"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..>1083
                     /locus_tag="AC16Y1_00440"
     CDS             62..>1083
                     /locus_tag="AC16Y1_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5830966.1"
                     /translation="MIDRRVTSEASGVVEQVLVKPGVIVESETVLVELSNPLLVRNLL
                     RSQSDLKVAEAKLVSLQAKLTDERLQYDFSIRQAQLEYESAQEIENAQRKLVDRNVVG
                     KLDFLKVLQATRVTKSRLKMLKERLPYLNKSHTAQLDSEKAFVEQLKYKVESSQRLVD
                     QLKIRAGLSGVLQDLTVEEGERLEIGSNIARVAKAWPLLAELDVQEVQAKDLTVGLKV
                     EIDTRNGKTMGEIVRVDPRVKAGNVQVDVQINELPKGTRPDQSITAIIEVEHIAQTLY
                     IDRPSSVSTQHQVSLFVLDDRKETAERREVKLGRTSVKLVEVLSGVAAGEKVIVSDVS
                     DFIEHPL"
BASE COUNT          338 a          187 c          283 g          275 t
ORIGIN      
        1 tagcgaagtg actgcggggg attttaaacg caaggtgaga ggggttggca aactggtttc
       61 aatgattgat cgtcgggtta catcagaagc aagtggcgtt gtagaacagg ttcttgtcaa
      121 acccggcgtt attgtcgaat cggaaacagt cttagttgag ttaagtaatc cattactggt
      181 taggaaccta ctccgaagcc agtcagactt aaaagtggcg gaagccaaac tggtctcttt
      241 acaggcaaaa ttaactgatg aaagattaca gtatgatttt tcaatcagac aggcgcagtt
      301 ggaatatgag tctgcgcaag aaattgaaaa tgctcagcgg aaattggtcg atcggaatgt
      361 tgtaggaaaa ttggattttc tcaaagtact ccaggcaacg agagttacta aatctcgact
      421 aaaaatgttg aaggagcgcc tgccatattt gaataaatca catactgcac aattggattc
      481 agaaaaagca tttgttgagc agctaaagta taaagtggag agtagtcaaa ggctagttga
      541 ccaacttaaa atcagagcag ggttgtcggg ggttttgcag gatttaaccg ttgaggaagg
      601 tgaacgatta gagattggaa gtaatatcgc tcgagtagcc aaggcatggc ctttgttagc
      661 tgagcttgat gtgcaagaag tacaggcgaa agatctcaca gtcgggttaa aagtagaaat
      721 tgatacccgt aatggaaaga ccatgggcga aatagttaga gttgacccaa gagttaaagc
      781 cggcaatgtg caagtcgatg ttcaaatcaa tgaacttccc aagggtacgc gaccagatca
      841 aagtattacc gcgatcatcg aagtagagca tatagcacaa acgctttaca tcgatcgtcc
      901 tagttctgtg tctacgcaac accaggtatc tctttttgtg cttgatgata gaaaagaaac
      961 tgctgaacgc agggaggtaa agcttggtag aacttctgtc aagcttgttg aagtgctaag
     1021 tggcgtcgca gcaggcgaaa aagtgatcgt gtctgatgtt agcgatttta ttgagcaccc
     1081 gct
//
LOCUS       DBNHVN010000027         3289 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_376661, whole genome shotgun sequence.
ACCESSION   DBNHVN010000027 DBNHVN010000000
VERSION     DBNHVN010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_376661"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="AC16Y1_00445"
     CDS             <1..899
                     /locus_tag="AC16Y1_00445"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5830967.1"
                     /translation="IGAAGIWAPQTTMLARMVPDEFRPKLFGLQFMMLNLGLGIGGII
                     SSFIVDINEPESFTRLFILDGISYLVFLSIIYTLREHGGPAPKELENENDGFRELLKD
                     KPFVKIQSVKLLLLTCGYASLDAGLPPLLTEFADLNINKLGPVWAVNTGVIVLGQIFV
                     INRLQGKSRTRLIGLIGLIWALSWAIVGVGVNANLALLGAFIGVGIFAVGEMIWSTIG
                     SSITNDLAPEHLRGRYNSADSLVWVTAAAMGPAISGFMLHNGWVNQWIGILVIGQLLG
                     GYFGIRMRKYLTPKQDGILVSN"
     gene            1116..2471
                     /gene="dnaB"
                     /locus_tag="AC16Y1_00450"
     CDS             1116..2471
                     /gene="dnaB"
                     /locus_tag="AC16Y1_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR5830968.1"
                     /translation="MSVAEIPRNYEGDGTPVPPQDLNAEQSVLGAMLLSKDAIADVVE
                     TLREVDFYKPAHATIYGAILDIYGRGEPADAITIANYLSKTGKLTNVGGAAYLHTLVA
                     SVPTAANASYYSRIVTERAILRRLVEAGTRIAQMGYSGDSEVDDLVDKAQAEVYEVTA
                     RRTSEDYAPLSDLIPNTLTELEAIEGNGGVAAGVPTGFSGLDEVTHGLHPGQLIILAA
                     RPAIGKSTLGLDLARNASIKNGMTSAIFSLEMSRNEIVMRLLSAEAQVSLGNMRAGTL
                     SEAEWQRLATKVGQVNDAPLFIDDSPNMTMMEIRAKARRLKQRFDLKLIVLDYMQLMS
                     SGRKVESRQVEVSEFSRSLKLLAKELEIPVVAISQLNRGPEQRQDKRPMLSDLRESGS
                     LEQDADMVILLHREDVYDRESPRAGEADFILAKHRNGPITTVVTAFQGHYSRFVDMAN
                     S"
     gene            2520..>3289
                     /locus_tag="AC16Y1_00455"
     CDS             2520..>3289
                     /locus_tag="AC16Y1_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5830969.1"
                     /translation="MPNIDRSKLNQLLERERTTYNERNPKSLALYQEATNLFGGVPMT
                     WMNKWSGGFPLYLDSAHGNTIKTVDGHTYIDFALGDTGAMAGHSPAPSTKAIAKRMMD
                     DGGITTMLPTEDAQWVGQELTRRFGMPLWSFTLTATDANRWAIRLARLATGKSKILVF
                     AYCYHGSVDETFAVPGAQGTAIARPGNVAPPVPLDETTRVVEFNDLAAVERELKNGDV
                     AAILTEPAMTNIGIVLPQPGFLEGLRELATKYGALLMI"
BASE COUNT          925 a          707 c          758 g          899 t
ORIGIN      
        1 cgattggcgc tgctggtatt tgggcaccgc aaaccacaat gcttgcgcgc atggtgccag
       61 acgaatttag accaaagtta tttggacttc agttcatgat gctaaatctt ggtttaggta
      121 ttggtggaat cattagctcg ttcatagttg acatcaacga accagaaagt ttcactcgac
      181 tatttatttt ggatggaatt tcatatttag ttttcttatc gattatttac actttgcgtg
      241 agcatggtgg accagctcca aaagaattag aaaatgaaaa cgatggcttt cgtgaattac
      301 ttaaagacaa accatttgta aaaatacaaa gtgtcaaact acttttatta acttgtggct
      361 acgcttcttt agatgcaggt ttgcctccgt tacttactga gtttgctgac ttgaatataa
      421 ataaacttgg accagtttgg gcagttaata ccggcgtgat tgtgcttggc caaatatttg
      481 taattaatcg cttacaagga aaaagtagaa ctcggttaat tggattaata ggtttgatct
      541 gggcactcag ttgggcaatt gtcggtgtgg gcgttaatgc gaaccttgct ttacttggcg
      601 cattcattgg tgttggcata tttgcagtcg gtgagatgat ttggtcaaca attggttcat
      661 caatcacaaa tgatttagcc ccagaacatt tacgtggccg ctacaactca gccgactcac
      721 tagtttgggt tactgcagcc gcaatgggtc cggcaatttc tggatttatg ctgcacaacg
      781 gctgggtaaa tcaatggatc ggcattttgg tgatcggtca gcttttgggt ggttattttg
      841 ggattcgaat gcgcaagtat ttaactccaa aacaagatgg aatattagtt agcaattaat
      901 ttttacccac aaatgttagg ttccatttcc cctgataatt cgccattttt tgactttgcg
      961 cggcgctaac cccacaagat atccccagtc aaaaaggcaa taaacacagg cttttcaaca
     1021 ctctgtccac atacttatcc acaggagtca ttgagtatgt ccgagccgga acataaagtc
     1081 atacctggtt ataactaatt attcaggtgg cttcaatgag cgttgcggaa ataccccgca
     1141 attacgaagg tgatggaact cctgttccgc cgcaagattt gaacgccgaa caatctgttc
     1201 taggcgcaat gctgctgagc aaagatgcaa ttgcagatgt cgttgaaaca cttcgcgaag
     1261 ttgattttta taaaccagca cacgcgacta tttatggcgc aattctagat atctatggtc
     1321 gcggtgaacc agctgatgca atcacgattg caaactactt atctaaaact ggaaaactaa
     1381 caaatgttgg cggcgctgcg tacctgcaca cattagttgc aagtgtgcca actgcagcaa
     1441 acgcttctta ctattcgcgc atcgttactg aacgagcaat cttgcgccgt ttagttgaag
     1501 ctggaactcg tattgcgcaa atgggttatt ccggtgattc cgaagttgat gatctagttg
     1561 ataaagcgca agccgaagtt tacgaagtaa ctgctcgtcg aacttcagaa gattacgcac
     1621 cactttcaga tttaattcca aacactttga ctgaacttga agcaattgaa ggaaatggtg
     1681 gcgttgctgc aggtgtgcct accggcttta gcggactaga cgaagtcacc catggcttac
     1741 atccaggtca attaataatt cttgctgctc gtccagcaat tggtaaatca acacttggtt
     1801 tagatttagc gcgtaacgca agtattaaaa acggtatgac ttcggcaata ttctcgctgg
     1861 aaatgagccg aaacgaaatt gtcatgcgcc tactttcagc tgaggctcaa gtctctttag
     1921 gtaacatgcg cgctggaaca ttaagtgaag ctgagtggca gcggttagct acaaaagttg
     1981 gccaagttaa tgacgctccg ttattcatcg atgattcacc aaatatgacc atgatggaaa
     2041 ttcgcgctaa agcgcgtcgg ttgaagcagc gctttgattt aaaactcata gtgctggact
     2101 acatgcaact tatgtcgagt ggccgaaaag ttgaaagtcg ccaagttgaa gttagtgaat
     2161 tttctcgatc gcttaagtta cttgctaaag aattagaaat tcccgtagtt gcaatcagtc
     2221 agttaaaccg cggacctgaa cagcgccaag ataagcgacc aatgctttct gacctacgtg
     2281 agtctggctc gttagaacaa gatgctgata tggttatttt gctacaccgc gaagatgttt
     2341 atgatcgtga atctccacgc gctggcgaag cagactttat tttggctaag caccgcaatg
     2401 gtccgattac caccgtggtt actgcattcc aaggccacta cagccgcttc gttgacatgg
     2461 ctaattccta aaaaaattaa gtctgtgtca gaataaaaac atccaaaagg ggtgttagcg
     2521 tgccgaatat cgaccgctca aaattaaatc aattattaga acgtgaacgc acaacttata
     2581 acgaacgaaa tccaaagtcg ctagcgctct atcaagaagc aacgaattta tttggtggcg
     2641 taccgatgac ttggatgaac aagtggtcgg gtggtttccc gctttattta gatagcgccc
     2701 acggaaatac aatcaagact gtcgatggcc acacatacat tgacttcgca cttggtgata
     2761 ccggtgctat ggctggtcac tcaccagctc caagcacaaa ggcgatcgct aagcgcatga
     2821 tggacgatgg cggaatcacc acaatgctgc caaccgaaga tgcgcaatgg gttggccaag
     2881 aattaactag acgttttggc atgccgctgt ggtcgttcac cttaaccgct accgatgcaa
     2941 acagatgggc aattaggtta gctcgcctgg caaccggcaa aagcaagatt ttagtttttg
     3001 cttattgcta ccacggctca gtggacgaaa catttgctgt tccaggtgcg caggggactg
     3061 cgatagccag accaggaaac gtagcgccac ctgtgccgct tgacgaaaca acacgggtag
     3121 ttgagttcaa cgatctagcc gcagttgaac gagaacttaa aaatggagac gtggctgcca
     3181 ttctgactga accagcaatg acaaatattg gcatcgtgtt accgcagcca ggtttcctcg
     3241 aaggcctgcg tgaacttgcg accaaatatg gcgcattact aatgatcga
//
LOCUS       DBNHVN010000028         1506 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_228358, whole genome shotgun sequence.
ACCESSION   DBNHVN010000028 DBNHVN010000000
VERSION     DBNHVN010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_228358"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="AC16Y1_00460"
     CDS             <1..382
                     /locus_tag="AC16Y1_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5830970.1"
                     /translation="ANTFFRSIGSTVGVAIFGTIYANRLATYIPEKVAELQTTNPQAL
                     VGATPEAFEALQTNSEVLQTFSPELQASILNAFVNSFHDVFLSALPITALGIVFAFMV
                     REKPLRSGQEFARAKEEAAGETLA"
     gene            complement(387..932)
                     /locus_tag="AC16Y1_00465"
     CDS             complement(387..932)
                     /locus_tag="AC16Y1_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5830971.1"
                     /translation="MNALPAFAPPTWSILVFYVLKYDLQAIALIPIGVVASTSGRGFL
                     ALYFRKLRNRLPNGWVANMENAGAHLRKSAKHSTALFLLFFISPFSSAQLFEAAGVMK
                     NQPLKPLLVSFAAGRLMTYTTYVAGAQAISASNFGDLLRQNVTSPGAIVIQVLMVVGL
                     VLLGNIKWKAPATQKSPAQSE"
     gene            complement(985..>1506)
                     /locus_tag="AC16Y1_00470"
     CDS             complement(985..>1506)
                     /locus_tag="AC16Y1_00470"
                     /inference="COORDINATES: protein motif:HMM:NF005754.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HIR5830972.1"
                     /translation="EVIRASRNSDVQVDVNSPESISNMFKQVGKLDAVAFAAGKVPFK
                     KLGELNRDDFVAGLTDKAIGQIDLVLKGTDYLTDDGSFTLMSGVLAREAILTGAVASA
                     ANGAIESFVMAASIELPRGIRINSISPNVLVESPGYHDYFPGFEQVKLSDVVRLYVKS
                     IEGPQTGQVYKLG"
BASE COUNT          444 a          331 c          319 g          412 t
ORIGIN      
        1 cgcaaacact ttctttaggt cgattggttc aaccgttggg gtggcaattt ttggcactat
       61 ctatgcaaac cgcttagcaa cttatattcc agaaaaagtg gccgaacttc agacgacaaa
      121 tccgcaggca ttagttggtg caaccccaga ggcatttgag gcgttgcaaa ctaattctga
      181 agtcttgcaa actttttctc cagagcttca agcatcaatt ttgaatgcat ttgtgaattc
      241 cttccatgat gtatttttat cggcgctacc gataactgca ttaggaattg tgtttgcgtt
      301 catggttcgt gaaaaaccgt tgcgctctgg ccaagaattt gctcgggcaa aggaagaagc
      361 agcaggcgaa actctggcct aaattgctat tcagattgcg ctggtgactt ttgggtcgct
      421 ggcgctttcc atttaatgtt gccgagtaaa accaaaccaa ccaccattaa aacttgaatt
      481 acgatcgcac ccggtgaagt tacattttgt cgaagcaagt ctccgaagtt actggcgcta
      541 atagcctgag cgcctgcgac ataagttgtg taagtcatta acctgcccgc ggcaaacgat
      601 actaacaacg gttttagcgg ttgatttttc ataactccag ctgcctcgaa gagttgagct
      661 gatgagaatg gcgaaataaa aaatagtaga aataaagctg ttgagtgttt agcagatttg
      721 cgaagatgtg ccccagcatt ttccatattg gcaacccagc cattgggtaa tcgatttctt
      781 aattttcgaa agtaaagcgc taaaaaaccg cgcccacttg tggatgcgac tacgccaatt
      841 ggaatcagcg cgattgcctg caaatcgtat ttaagtacat aaaaaactaa aattgaccag
      901 gttggtgggg caaatgctgg gagtgcgttc ataataaaaa ttacgagcgc agcaattaaa
      961 tagatttcca aactagctca cgaattagcc aagtttgtaa acttgaccag tctgtggacc
     1021 ttcaattgat ttaacgtaaa gccgaactac atcactaagt ttgacctgtt caaaacctgg
     1081 aaaataatca tggtagccag gggactccac aagtacgttt ggactaattg agttaattcg
     1141 aatccctcta ggtaattcaa ttgatgcagc cattacaaaa gattcaattg cgccatttgc
     1201 cgctgatgca accgcaccag taaggattgc ttcgcgagca agtactcccg acatcaacgt
     1261 gaatgaacca tcatcggtta aatagtcagt tcccttcagc actagatcaa tctgcccaat
     1321 agccttatcg gtaagtccag caacgaaatc atcacgattt agttcaccga gcttcttaaa
     1381 tggaactttg ccagcggcga aagcaaccgc atctaatttg ccgacttgct taaacatatt
     1441 tgagattgat tctggactat tgacatcaac ttggacatcg ctatttcgag aagcacgaat
     1501 tacttc
//
LOCUS       DBNHVN010000029         4778 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1194354, whole genome shotgun sequence.
ACCESSION   DBNHVN010000029 DBNHVN010000000
VERSION     DBNHVN010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4778)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4778)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4778
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1194354"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="AC16Y1_00475"
     CDS             complement(<1..1243)
                     /locus_tag="AC16Y1_00475"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF013790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase"
                     /protein_id="HIR5830973.1"
                     /translation="MAIPKAVQNKHAKLVEQIEQARTDYYQRDKPTISDDEYDKSFKE
                     LVELELKYPELASGDSPTQSVGGQAAEGFTEFKHPTRMWSLDNVFDDDELASWFNRVG
                     NHDFLCELKIDGLAINAVYRNGKLETLATRGSGSVGENVTANVEYMKCIPTELKNSKG
                     KKVPELLEVRGEVFFTLAEFDQINLEVTATGRTAFANPRNAAAGTLRQRIDRRIEAVN
                     DATKRADGKAAESKQVASLAKYQEELDRAKTALSRLDLIVHGIGAHEGLEITAQSHAY
                     EILNEWGLPTSNRVEVVKSAKEVQAYIAKFGEQRHEIEHDIDGVVIKVDSLAEQTELG
                     FTARAPRWAIAFKYPPTVVRTKLVDIGVQVGRTGRITPRAQVEPVLVAGTTVSFATLH
                     NQAEILRKGVLIGDTIFLRKAG"
     gene            complement(1246..2361)
                     /gene="mnmA"
                     /locus_tag="AC16Y1_00480"
     CDS             complement(1246..2361)
                     /gene="mnmA"
                     /locus_tag="AC16Y1_00480"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006346417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR5830974.1"
                     /translation="MRVLAALSGGVDSAVAAARAVDAGHDVTAVHLALSRNRESLRTG
                     ARGCCTIEDANDAFRAADMLGAPFYVWDLSAEFQEAVIDDFVSEYAAGRTPNPCLRCN
                     ERIKFQAVLERAMALGFDAVVTGHYAQVFNGAHGKELHRAIDMTKDQSYVLGVLTHEQ
                     ISHSLFPLGDTTKEEVRKEAQSRGILVAQKPDSHDICFIPDGDTAGWLARKIGEKPGE
                     VVDAVSGEVVGQHEGAHAYTVGQRRGLALGRPAPDGARRYVVDVDVPNNKVMVGPPTL
                     LDINVIEAIRPVWIGPTLVDGMKVGVQFRAHGEEIPATAFNTQSGFRIELEKMARGVA
                     SGQAAVLYDGTRVVGSGTINRTRRNSVAPTEQLVGSK"
     gene            complement(2361..3551)
                     /locus_tag="AC16Y1_00485"
     CDS             complement(2361..3551)
                     /locus_tag="AC16Y1_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR5830975.1"
                     /translation="MAYLDHAATTPMYSEVSQLMAKLLTDCGNPSSLHASGRRARRLV
                     EEARESVANSLGCRPAEVVFTSGGTEANNIALKGIAWDQRDKDSKRNRVLISAVEHHA
                     ILDPANWLGESDDFRVEYIPVTSSGLVTPDALARTINDDFDSVAVVSVMLANNEVGTI
                     NNIAALVDVIRGRGIRFHTDAVQAPLWMDVNFSDLGTCAMSVSGHKVGGPHGVGAIIM
                     SRDCTVTPLIHGGGQERDVRSGTLDVPAIVAFAKALEISVRQRSEQVSRITKLRDELI
                     DQVKAVVPDAILNGDRQARLANNVHFTFPGCLGDSLLMLLDANGVECSVGSACSAGVP
                     QPSHVLLAMGADEDSARCTLRFSLGHNSTSQDLTELITYLPSAVERAKRAGVPKSFAM
                     AGEE"
     gene            complement(3596..4543)
                     /locus_tag="AC16Y1_00490"
     CDS             complement(3596..4543)
                     /locus_tag="AC16Y1_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR5830976.1"
                     /translation="MADVLVLVEHTKGEVKKVSLELLAMAAKVGSPVAVALGPGADST
                     SGVLGEYGAKKVYVDTSDVYVNEVVVPAVTALKKLVAELAPAAVVVPSSADGKEIAGR
                     LAVATNSGVITDASEISSDGSVTQNIFGGATIVTARVTLGTPIVTVRPNSITPVASGG
                     SAEVIALSVDHPASKTKVTNRVSVQKSGRPDLSEAAVVVSGGRGVASEEGFKNVIEPL
                     ADALGAAVGASRAATDAGWYPHQFQVGQTGKTVSPQLYIANGISGAIQHRAGMQTSKT
                     VVVVNKDPEAPIFDLADFGIVGDLNEVVPDLTTRIKARK"
     gene            complement(4552..>4778)
                     /locus_tag="AC16Y1_00495"
     CDS             complement(4552..>4778)
                     /locus_tag="AC16Y1_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HIR5830977.1"
                     /translation="YPSFKNIMAAKKAPMETLSLAEVGIDPSAVGLSAAWSAVSSFTA
                     RPAKAAGTKVVDDGNAAAQLEEFLVAQKFI"
BASE COUNT         1261 a         1198 c          980 g         1339 t
ORIGIN      
        1 ctccagcttt acgtaaaaat attgtgtcgc caatcagtac gcctttgcgc aaaatttctg
       61 cttggttatg caaagttgca aagctaacag ttgtaccagc aactagaact ggttcaactt
      121 gtgctcttgg tgtaattcga ccagtacggc caacttgcac gccaatgtca actaactttg
      181 tgcgcacgac agttggtgga tacttaaagg caattgccca gcgcggtgcc cgagcggtaa
      241 agccaagttc agtttgttcg gctaagctat caacttttat tacaacgcca tctatgtcat
      301 gttcaatttc atgacgctgt tcgccaaatt tagctatgta cgcttgcact tctttagccg
      361 acttaacaac ttcgactcga ttcgaagttg gtaaacccca ttcatttaaa atctcataag
      421 catgtgactg tgcagtgatt tccaaaccct cgtgcgcccc aataccgtgc actattaagt
      481 ccagccgact caaagctgtt ttagctcggt caagttcctc ttgatactta gctaaagatg
      541 cgacttgttt actctctgcc gctttaccat cggcccgttt cgttgcatcg tttactgcct
      601 caatgcgccg atcaattctt tggcgcaatg taccggcagc agcatttctt gggttggcaa
      661 atgcagttct acctgtcgca gtcacctcaa gatttatctg atcaaactcg gcaagagtaa
      721 aaaatacttc accacgaact tcaagtagtt ctggaacttt tttccctttg gaatttttta
      781 actcagttgg aatgcacttc atgtactcga catttgcggt gacattttcg ccaactgaac
      841 cactacctct agtggctaat gtttcaagct tgccatttcg ataaaccgcg ttaatggcta
      901 aaccatctat ttttaattca cataagaagt catgatttcc aactcgatta aaccaagatg
      961 caagttcatc atcgtcaaac acattgtcta atgaccacat ccgggttgga tgtttaaatt
     1021 cggtaaagcc ttctgctgcc tgccctccaa cggattgggt gggtgaatca ccggaggcta
     1081 actctggata tttaagttca agttcaacta attctttaaa tgatttgtca tattcgtcgt
     1141 cagaaatagt tggcttatct cgctggtaat aatccgtgcg agcttgctct atttgctcaa
     1201 ccagtttggc gtgtttattt tgaaccgcct tagggattgc cacgattatt ttgatccaac
     1261 taattgctcg gtaggcgcaa cagaattgcg gcgggtgcga tttatagttc ccgatccaac
     1321 aactcgagtt ccgtcatata agactgctgc ttggccactt gctactccgc gagccatttt
     1381 ttctaattcg attcgaaaac ctgattgcgt gttaaatgcg gtggctggaa tttcttcgcc
     1441 atgtgcgcgg aactgaacgc caactttcat cccgtccacg agagttggtc caatccagac
     1501 cgggcgaata gcttcaatga cattgatatc tagcaaagtt ggcgggccca ccataacttt
     1561 gttgttcggc acatctacat caactacata tctacgagca ccatctggag caggtcgacc
     1621 aagtgctaaa cctctgcgtt gaccaactgt gtatgcatga gcgccctcgt gttggcccac
     1681 aacttcgcca ctgacggcat caacaacttc accgggcttt tcaccaattt tgcgcgctaa
     1741 ccaacccgcg gtgtcaccgt ctggaatgaa gcaaatgtca tggctatctg gtttttgggc
     1801 aactaaaatt ccacgggatt gcgcctcttt gcgaacttcc tcttttgttg tatctcccaa
     1861 tggaaaaagt gaatgtgaaa tttgctcgtg agttagcacg ccgagaacat aagactgatc
     1921 tttagtcata tctattgctc gatgtaactc tttgccatga gctccgttaa aaacttgggc
     1981 gtaatggcca gtaactaccg catcaaaacc taatgccata gcacgttcta aaactgcttg
     2041 gaatttaatt cgctcattgc aacgaaggca cggattaggt gttcggccgg cggcgtattc
     2101 actgacaaaa tcatcaatta cagcttcttg gaattcagct gaaagatccc aaacataaaa
     2161 aggtgcacca agcatatctg ctgcgcgaaa tgcatcattt gcatcttcga tagtgcagca
     2221 tcctcttgca ccagttcgta atgattcgcg attccgtgaa agcgccaaat gaactgcggt
     2281 cacatcatgg cctgcatcga cagcgcgcgc tgccgcaacg gctgagtcaa ctccaccaga
     2341 aagtgcagca agtactcgca ttactcttcc cctgccattg cgaaagattt tggcactccg
     2401 gcacgtttgg ctcgctcgac tgcactcggt aaataagtaa ttaattcagt tagatcttga
     2461 cttgttgagt tatggcctaa tgagaagcgc aaagtgcatc gcgctgaatc ttcgtcagcg
     2521 cccatagcca acaaaacatg agatggctgg ggtactccag cgctacaagc cgaacccaca
     2581 gaacattcaa caccatttgc atcaagcaac ataagtaatg aatcacctaa acaacctgga
     2641 aaagtgaagt gcacattatt tgctaggcgt gcttgacgat cgccatttaa aatcgcatct
     2701 ggcacaactg cttttacttg atcaattagt tcatcgcgta atttagtaat tcttgaaact
     2761 tgttcacttc tttgtcgaac cgaaatctct agtgcttttg caaacgcaac aatcgctgga
     2821 acgtctaaag ttcctgatct aacatcgcgc tcttggccgc caccatgaat gagcggagtc
     2881 actgtgcaat ctcggctcat gatgatcgca ccaacaccgt gtggtccacc aactttatga
     2941 cccgaaacgg acatggcaca ggtacctaaa tctgaaaaat taacatccat ccaaagcgga
     3001 gcttgaacag catctgtgtg gaatctaatc ccacgaccac gaattacgtc aacaagagca
     3061 gcaatattat ttatggtgcc aacttcatta ttggctaaca tcacacttac aactgccact
     3121 gaatcaaaat catcatttat tgttctggca agtgcatccg gtgttactag tccgctcgaa
     3181 gttactggaa tgtattcgac tcgaaagtca tcactttcgc caagccagtt agctgggtca
     3241 agtattgcgt gatgctcaac agcactaatt agaactcggt tgcgttttga atctttatct
     3301 cgctggtccc aagcgatgcc tttcaaagca atgttgtttg cttcagtgcc acctgatgta
     3361 aaaacaactt cagccgggcg gcaacccagc gagtttgcca cactttcgcg agcttcttcg
     3421 actaatcgcc ttgcccggcg cccggaggca tgcaaagaag acggattacc acaatccgtc
     3481 aataactttg ccattagctg cgaaacttcc gagtacatcg gagttgttgc cgcatgatcc
     3541 aagtacgcca ctagttaatt ctacctattt atgtaagtgg cggaattagc agaatttact
     3601 tacgagcctt aattcgagtt gttaaatctg gcacaacctc atttaagtcg cctacgattc
     3661 cgaaatcggc taaatcaaaa ataggtgctt ctgggtcttt attgaccaca acaactgtct
     3721 ttgaagtttg cattccagcg cgatgttgga ttgctccaga aattccattg gcaatataaa
     3781 gctgaggcga aactgtttta cccgtttgcc caacttggaa ttggtgtggg taccagccag
     3841 catctgtggc agcgcgtgat gcgccaactg cagcacctag tgcgtcagca agtggctcaa
     3901 ttacattctt aaagccttct tcactggcca caccacgtcc accagaaaca accacggcag
     3961 cttcggatag atcaggacga ccactctttt gtaccgaaac tcgattcgta accttggttt
     4021 ttgaagctgg gtgatcaacc gataaagcaa taacttccgc agatccacct gatgcaactg
     4081 gagtaatgga attagggcga actgtaacaa ttggagtgcc cagggttact cttgccgtga
     4141 caattgtggc gccaccaaaa atattttgcg taactgaacc atctgaactt atttctgatg
     4201 catctgtaat aacaccagaa ttagttgcta ctgcaagtcg tccagcgatt tcttttccgt
     4261 ctgcacttga tggaactacg actgcagctg gggctaattc tgctactaat ttttttaatg
     4321 cagtaacagc gggcactact acttcattta cataaacgtc actcgtgtct acataaactt
     4381 tctttgcacc atattcgcca agcaccccgg atgttgaatc cgcacctggt ccgagtgcaa
     4441 cagctactgg agatccaact ttggcagcca tcgccagtaa ttcaagtgaa acttttttta
     4501 cttcaccttt tgtgtgttca actagaacta atacatcagc cataattttc cttaaataaa
     4561 cttctgagct actaaaaatt cttcaagctg tgctgcggcg ttaccatcat caacaacttt
     4621 tgtaccggcc gcctttgctg ggcgtgccgt aaagcttgaa acggctgacc aagccgcgga
     4681 taaaccaact gcactcggat caataccgac ttcggctagc gaaagtgttt ccatcggtgc
     4741 cttcttcgct gccatgatgt ttttaaaaga tggatagc
//
LOCUS       DBNHVN010000030         1178 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1640064, whole genome shotgun sequence.
ACCESSION   DBNHVN010000030 DBNHVN010000000
VERSION     DBNHVN010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1640064"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="AC16Y1_00500"
     CDS             <1..606
                     /locus_tag="AC16Y1_00500"
                     /inference="COORDINATES: protein motif:HMM:NF037316.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HIR5830978.1"
                     /translation="HVKINTLPTVRTDPWRRLSGIHKVKDRRQMAIARSLWQARDEVA
                     KHLDISSGRILNDSHIIAISEQPNWSKVLELPFMKLRIIQRNLKTWQQAFHSGLNLSH
                     DELPELKIKSDSPPHPRGWQTRHPELFAKLEKIRTDLAKLAEDLVLPVENLISPETIR
                     RAVWHKPTDLESLHKIFAENKVRNWQRELVSQIVVEALEIN"
     gene            complement(603..>1178)
                     /locus_tag="AC16Y1_00505"
     CDS             complement(603..>1178)
                     /locus_tag="AC16Y1_00505"
                     /inference="COORDINATES: protein motif:HMM:NF014799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR5830979.1"
                     /translation="LALLRLVPTLELAAPRDGEQLKIALNKAVEKSDHPTVVRFPKGA
                     LVSPVVAQRTLPYGDVLLENADPDVTIISIGALAGEVVKAAQILKTEGYQVRVIDPVW
                     ALPINPALVADVQNSSLLITVEDGLVSGGIGEAIASALHNLNLDVPVVNLGIAKEFIP
                     QANRSVLLSKFGLDSLSIANTVRKKLQATVG"
BASE COUNT          372 a          248 c          241 g          317 t
ORIGIN      
        1 catgtgaaaa taaatacatt gccaacagtc cgaacagacc cttggcgaag attatcgggc
       61 atccataaag tcaaagatcg ccggcaaatg gcaattgcgc gatccctttg gcaggctcgt
      121 gatgaagtcg caaagcactt agacatctca agtggtcgca tcctgaatga cagtcacata
      181 attgcgatta gtgagcagcc aaattggtct aaggtgttgg aattaccgtt tatgaagtta
      241 agaattattc aacgtaactt aaaaacttgg caacaggcat ttcattctgg tttaaacctt
      301 agtcacgatg aattacctga attaaagatt aaatccgaca gcccaccaca tcctcgtggt
      361 tggcaaacaa gacatccaga actatttgcc aaattagaaa aaatcagaac agatttagct
      421 aaattagctg aagatcttgt cctgccagta gaaaatttga tttctccaga aactattcga
      481 cgagctgtat ggcataaacc aactgatttg gaatcactgc acaaaatttt tgctgaaaat
      541 aaagtacgta attggcagcg tgaattagta agtcaaattg tggttgaggc cctagaaatt
      601 aattagccca cagttgcttg cagttttttg cgaacagtat tggcgatact tagcgaatcc
      661 aacccaaact tagatagtaa aactgaacga tttgcttggg gtataaattc ctttgcgata
      721 ccaagattca caactggtac atctagattc aagttgtgta aggcgctcgc gatagcttcg
      781 ccaatgccac ctgaaactaa accatcttcc acagtgatta gcaaagagct attttgaaca
      841 tcagcaacca aggctgggtt tattggcaat gcccaaactg ggtcgataac tcggacttga
      901 taaccttcag tttttaaaat ttgggccgct ttaacaactt ctccggcaag tgcgccaatt
      961 gaaataatcg ttacgtcagg atctgcattc tcaagcaata catcaccgta tggcaaagtt
     1021 ctttgcgcaa ctacaggtga gactagtgcg ccctttggaa accggacaac tgttggatga
     1081 tcagattttt caactgcttt atttaaagca attttcaact gctcaccgtc acggggtgcg
     1141 gctaattcca aagttggaac taaacgcaga agtgcaag
//
LOCUS       DBNHVN010000031         2743 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1862954, whole genome shotgun sequence.
ACCESSION   DBNHVN010000031 DBNHVN010000000
VERSION     DBNHVN010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2743
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1862954"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1520
                     /locus_tag="AC16Y1_00510"
     CDS             <1..1520
                     /locus_tag="AC16Y1_00510"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009341316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR5830980.1"
                     /translation="AKPFIRILCEAQIELAKVAAKETAEFPVFLDYEADAFVAVEELV
                     SEDLAKALTISGKQERETELDRIKDLTVEKLGAQFEGREKEISAALRSVTKKLVRKRV
                     IKDKIRIDGRGLRDIRNLTAEVDVIPRAHGSAIFERGETQIVGVTTLNMLKMEMQLDT
                     LSPITSKHYMHNYNFPPYSTGETGRVGTPKRREIGHGALAERALVPVLPAKADFPYAI
                     RQVSEAFGSNGSTSMGSVCASTLSLLNAGVPLKAPVAGIAMGLISDEVDGKTEYVALT
                     DILGAEDAYGDMDFKVAGTKDFVTALQLDTKLDGIPASVLAAALTQAREARLAILDVM
                     LEAIDAPDEMSPYAPRIISVQIPIDKIGEVIGPKGKIINQIQEDTGADLTITDDGIVY
                     IGATSGEAAEAARAQVNAIANPSMPEVGERYMGTVVKTTNFGAFISLSPGKDGLLHIS
                     QLRKLSGGKRVENVEDVVKVGDKVHVEIAEIDQRGKLSLIPVVEGEESSTDEVDD"
     gene            1521..>2743
                     /locus_tag="AC16Y1_00515"
     CDS             1521..>2743
                     /locus_tag="AC16Y1_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010471507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR5830981.1"
                     /translation="MSKSKLKVVLGSQQLPGTTRTLLTESQGGLVKRTVLPSGIRIIT
                     EKMPTVRSAAIGIWVNVGSRDEIAAQTGSAHYLEHLLFKGTKTRSALDISSAIDAVGG
                     EMNAFTSKEVTCFYTRVLDTNVPLAIDVLADMITSATITTADVDQERTVVLEEIAMRD
                     DDPADIVHEQFAKAMYGETSLGRSILGTVENISSLTRRSIFGFYQKYYTPDRLVISVA
                     GNVDHAQIVKLIRNAFAKGGMDLGATEDAPFVAENKKAKVNRNGGLVKFNKDTEQANV
                     VIGVPGIDRADERRYILNVLNSVLGGGMSSRLFQEVREKRGLVYTVYSFAQQFQDAGM
                     VGIYAGCNPKNLTTVREVCSNVLADLVKNGITEAELQKAKGQVSGAMVLGLEDTGSRM
                     SRNARSELNYGYLTSV"
BASE COUNT          801 a          581 c          650 g          711 t
ORIGIN      
        1 cagccaaacc atttattcga atcctttgcg aagctcagat cgaactagcc aaagttgctg
       61 ctaaagaaac tgctgaattt ccagttttcc tagattacga agcagacgca tttgttgcag
      121 ttgaagaatt agttagtgaa gatcttgcca aagcattaac gatttctgga aaacaggaac
      181 gcgaaacaga gcttgatcga attaaagatc taactgttga gaaacttggt gcccaatttg
      241 agggtcgcga aaaggaaatt agcgcggcac ttcgaagtgt gactaaaaag ctagttcgca
      301 aacgggttat caaagataaa attcgtattg atggccgtgg attacgcgat attcgtaatt
      361 tgactgctga agttgatgta attccaagag ctcatggctc agcaatattc gaacgtggcg
      421 aaacccaaat tgttggtgta actacgctca atatgctcaa aatggaaatg cagttggata
      481 ctttgtcgcc aattacttca aagcactaca tgcataacta caactttccg ccttactcaa
      541 ctggtgaaac tggtcgagtt ggcacaccta agcgccgcga aattggacac ggtgctttgg
      601 cagaacgcgc tttagttcca gtgctaccag cgaaggctga tttcccatac gcaatccgcc
      661 aggtatctga agcattcggt tcaaacggtt caacttctat gggttcagta tgtgcctcaa
      721 cattgtcact actaaatgca ggtgttccac taaaggcacc agtagccggt attgcaatgg
      781 gtctaatttc agatgaagtt gatggcaaaa ctgagtatgt tgcattaacc gatattcttg
      841 gcgcagaaga tgcttacggt gacatggact tcaaggttgc tggtactaag gactttgtga
      901 ctgcattgca gttggatacc aagctagatg gcattccagc atctgtactt gctgctgcat
      961 taactcaggc tcgcgaagct cgcttagcga ttcttgatgt aatgcttgaa gcaatcgatg
     1021 ctccagatga gatgtcacca tatgcaccac gcattatttc ggtgcagatt ccaatagaca
     1081 agatcggtga agttatcggg cctaagggca agatcatcaa ccagattcaa gaagatactg
     1141 gtgctgactt aacaatcact gatgacggca ttgtctacat cggagccacc agtggcgaag
     1201 cagctgaagc agctcgcgca caagttaatg cgattgctaa cccatcaatg ccagaagttg
     1261 gcgaacgtta tatgggcact gtagtcaaga caactaactt cggtgctttc atttccttgt
     1321 ctccaggtaa ggatggactg ctacacatca gtcagttacg taaactttct ggtggtaagc
     1381 gtgttgaaaa cgtagaagat gtagtcaaag ttggcgataa agtccacgtt gaaattgctg
     1441 aaatcgatca acgcggaaag ctttcattaa ttccagttgt tgaaggcgaa gagagctcaa
     1501 ctgacgaggt tgacgactaa ttgtcaaaaa gtaagttgaa agtggtcctt ggttcgcaac
     1561 aattaccagg gaccactcgc actttattaa cggaaagcca aggcggctta gttaagcgca
     1621 cggttctgcc aagtggaatc cgaattatca ccgaaaaaat gccaacggtt cgttctgccg
     1681 ctatcggcat ttgggtaaat gtcggctcac gcgatgaaat tgcggcgcaa actggatcgg
     1741 cacattatct cgagcacctt cttttcaaag gtacaaaaac tagaagtgca ctggacatca
     1801 gctctgccat agatgcagtt ggcggagaaa tgaatgcctt tacttccaaa gaagttacct
     1861 gtttttatac ccgagtatta gatacaaatg tcccgttggc gattgacgtc ctggcagaca
     1921 tgatcactag cgccacaatc actacggctg atgttgatca agaacgcaca gtcgtcctcg
     1981 aagaaattgc aatgcgtgat gatgacccag cagacatcgt gcatgagcag ttcgctaagg
     2041 caatgtacgg tgaaacttcg ctaggtcgat cgatcctggg cacagtcgag aacatctcat
     2101 ctttaacccg acgctctatt ttcggttttt atcagaagta ctatacgccg gatcgattag
     2161 taatctctgt tgccggaaat gttgaccatg cccaaatcgt caaactaatt cgcaatgcat
     2221 ttgccaaggg cggcatggac ttaggtgcca cagaagatgc gccatttgtg gctgaaaata
     2281 aaaaagcaaa agtcaatcgc aatggtggct tagttaaatt taacaaagat actgagcagg
     2341 ccaacgtcgt tattggcgta ccaggtattg atcgcgctga tgaacgacgc tacatactaa
     2401 atgtcttgaa ttcagtttta ggtggtggaa tgtccagtcg actattccaa gaagtgcgcg
     2461 aaaaacgtgg cttggtttac accgtttact cattcgcgca acaatttcaa gatgctggaa
     2521 tggttggtat ctacgcaggt tgtaatccaa aaaatttaac tactgttcgc gaagtatgtt
     2581 caaatgtgct tgctgatcta gttaaaaatg ggattaccga agcagaatta caaaaagcaa
     2641 aaggtcaagt aagcggtgca atggtgctgg gtcttgaaga caccgggtca cggatgagcc
     2701 gaaatgcccg tagcgaacta aattatggct acctaacttc ggt
//
LOCUS       DBNHVN010000032         3031 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1045981, whole genome shotgun sequence.
ACCESSION   DBNHVN010000032 DBNHVN010000000
VERSION     DBNHVN010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1045981"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="AC16Y1_00520"
     CDS             <1..421
                     /locus_tag="AC16Y1_00520"
                     /inference="COORDINATES: protein motif:HMM:NF020397.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HIR5830982.1"
                     /translation="KQIDGLRKLIKSNLQPGFEESVNWGMLVYQVPIKRCPETYNGQP
                     LAYVGLAAQKNYLALYLLGIYSDEKLRKKFETHYKKSGKKLNMGKSCLRFKSIDEIPV
                     DVIAESISEFSVEDFIEMHKKSMSHRKSKKLKKIQQA"
     gene            complement(579..942)
                     /gene="ssrA"
                     /locus_tag="AC16Y1_00525"
     tmRNA           complement(579..942)
                     /gene="ssrA"
                     /locus_tag="AC16Y1_00525"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            1115..2251
                     /locus_tag="AC16Y1_00530"
     CDS             1115..2251
                     /locus_tag="AC16Y1_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014234448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HIR5830983.1"
                     /translation="MATSKDQLINWRHHLHSIAEPAFQEVETANYLENELKKLGLSVE
                     SNVGKTGLVATLQKGNSSKSIGIRADIDGLPIQEQTGLSYAANDRSAMHACGHDGHMT
                     MALGAAARLVEEEFDGIIHFIFQPAEEPGYGAKAMIEDRIFERFPVDEIYGIHNIPGL
                     PAGHLVTRSGPIMASEDNFIIQINGVGGHASAPQRVIDPLVIAAQIITALQTIVSRSV
                     DPLQSAVVSCTEIFTDGARNAIPGEVIIKGDTRTFDKEIQKLVEQRMIEIVEGICSAN
                     GAKGTVEYTHEFEPTINDSECAKFAYECAKQVVGPNESSVECAPITASEDFGVFAESV
                     PGCFVFLGTGEDHTPLHNKYYNFNDEVLEVGVNFYTQLVKAKLA"
     gene            2305..>3031
                     /locus_tag="AC16Y1_00535"
     CDS             2305..>3031
                     /locus_tag="AC16Y1_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5830984.1"
                     /translation="MTNNTENSTSTQKQPGKFATYFGMAAPYNFSIALPALFGFFLLG
                     CVTILAVGMAATDRLSFGAPRSYFFIYLFALLFLALILVKKPKVTWTLLVLVFIEFFL
                     AFIPYFLNRLGLSLNANLLPAAANSRFEYHPLLQGVPKPGYSGTLPDGGILTHTDFGT
                     RGPQPSADVLNSGILINAFGGSTTYDVAVSDEQSWPNQLGTLLGSNVAVINRGVPAYT
                     SADNLIQTMFYDQVNNRTPPCALY"
BASE COUNT          887 a          617 c          664 g          863 t
ORIGIN      
        1 caagcaaata gatggcctgc gcaaattaat aaaaagtaat cttcaacctg gctttgaaga
       61 gtcggtgaac tgggggatgc tggtttatca agttcccatt aagcgctgtc ctgaaaccta
      121 caacggtcaa ccactggcgt acgtaggact tgctgcacag aaaaactatt tagctcttta
      181 tttgttggga atttatagtg atgagaaact acgcaaaaaa ttcgaaacac actataaaaa
      241 atcaggcaag aaattaaata tgggaaagtc ttgtctcaga ttcaaatcta tagatgagat
      301 accggttgat gtaattgcag aatcaataag tgaattttca gttgaggact ttattgagat
      361 gcacaaaaag tcaatgtcgc acagaaaaag caaaaaactc aaaaagatac agcaggcata
      421 ggcatcaggt taaattcgat tcatcagatc ttgaattatt gcgaactaga aaccttcgct
      481 ggtgcaaaaa ccaagggact caattccatc tcctcccgat tcccgttgca tttccaaaat
      541 taaaaaacgg tttacccgaa catgggtaaa ccgtttttaa gtggagatgc cgggaatcga
      601 acccgggtcc ttaggtgaag atttggttat tctccgagcg cagtttggat tagcttttct
      661 ctgccccgac gttcacccaa acgagatgtc gacaggcaca gctactgttt aatttccttt
      721 gatcacccgt aaccggcaat cgcagtaagt ttcctaatga cgataggttc cagagcggaa
      781 acgcccctgg gctatcggat ttcggggcta gcttatgcag ctagggcgaa atcagtgcgg
      841 ttttgtttgg cacttatagt tttgctggac ttttacgaga tgaccaacat tctcggctcg
      901 cttctcccat ttcgactacc aaagtcgaaa ccgttcatcc ccaagttttt gggggttaac
      961 gagtgagtta actccactat ttaattatgt gaacaattct aagcgaagtg tctgaaggtt
     1021 aaaacgccaa acaaggacta cttattccct acgcactttt gaataaagcc acaccccagt
     1081 ttttgaggga atttgggcgg attaaagtca atctatggcc acttcaaaag accaacttat
     1141 taattggcga caccatttac acagcatcgc agagccagct ttccaggaag tcgagactgc
     1201 taattattta gaaaacgaac ttaaaaagtt gggactttcc gttgaaagta acgtcggaaa
     1261 aactggttta gttgcaactt tacaaaaagg taatagcagc aaaagtattg gaatccgagc
     1321 cgacatagat ggtttgccga ttcaagaaca aacgggttta agttatgcag ccaatgatcg
     1381 ttcagccatg catgcctgtg gtcacgatgg acacatgacc atggctcttg gcgccgcagc
     1441 tcggttagtc gaagaagagt ttgacggaat aattcatttt atttttcagc ctgccgaaga
     1501 accaggttat ggcgcaaagg caatgatcga agaccgtatt tttgaaagat ttccagtaga
     1561 tgaaatttat ggaattcaca atattccggg tttacctgct ggtcatttag taaccaggag
     1621 cgggccgatt atggctagcg aagataattt catcattcag attaatggag ttggcggcca
     1681 tgcatctgca ccacaacggg taattgatcc gttagtaatt gcagctcaaa taattacggc
     1741 tttgcaaaca atagtgagta gaagtgttga tccattgcag tctgcagtag tttcctgcac
     1801 tgaaatcttc actgatgggg cccggaatgc cattcctggg gaagtaatta ttaagggcga
     1861 cactagaact ttcgacaaag aaattcaaaa actggttgaa cagagaatga ttgaaattgt
     1921 tgagggaata tgtagcgcga atggcgctaa gggaactgtt gagtacacac atgaatttga
     1981 gccgactatt aatgattctg agtgtgcgaa gtttgcctat gagtgtgcaa aacaagttgt
     2041 tggacccaac gaaagttctg ttgagtgtgc tccgataact gcaagtgagg attttggagt
     2101 gttcgctgaa tccgttccag gatgctttgt atttttgggc accggtgaag atcacacacc
     2161 attacataac aagtactaca acttcaatga tgaagtcctt gaggtaggtg tcaattttta
     2221 tacgcagctg gtgaaagcta aattggctta agtgatgaag gtatactcga agagccctaa
     2281 caaagtgatt tgaaagttag tgaattgact aataacactg aaaactcaac atcaacccaa
     2341 aagcagcctg gcaaatttgc aacttatttt gggatggcgg ctccgtacaa cttttcaata
     2401 gcgctaccgg ctttatttgg ctttttcttg cttggttgcg tcacaatcct ggccgttggt
     2461 atggctgcca cagatcgatt aagttttggt gctccccgaa gttacttctt tatctacctg
     2521 ttcgcactac tcttcttggc tctcatttta gttaagaagc caaaagtcac ttggacactt
     2581 ttagttttag tctttattga attcttttta gcttttattc cttatttttt aaatcgctta
     2641 ggtctgagtt tgaatgcaaa tttattacca gcagcagcga attctcgttt tgaataccat
     2701 ccactactgc aaggtgtacc taagcctgga tattcaggaa cactacctga cggcgggatc
     2761 cttacccaca ccgattttgg aactcgcggc ccacagccaa gtgcagacgt cttaaactct
     2821 ggaattctga tcaatgcatt cggaggttca acaacctacg atgttgcagt cagcgatgaa
     2881 cagtcttggc cgaatcaatt aggtacatta cttggttcaa atgttgcagt gattaatcga
     2941 ggtgttcctg cctacacttc tgcagacaat ttaattcaaa cgatgtttta tgaccaggta
     3001 aataatcgaa cgcctccttg cgcactttat t
//
LOCUS       DBNHVN010000033         1575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1343349, whole genome shotgun sequence.
ACCESSION   DBNHVN010000033 DBNHVN010000000
VERSION     DBNHVN010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1343349"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1575)
                     /locus_tag="AC16Y1_00540"
     CDS             complement(<1..>1575)
                     /locus_tag="AC16Y1_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HIR5830985.1"
                     /translation="PICYSAISGDVSVLHVPGVPSSISNPSTDTDGSITVSWGSATGR
                     AADYRLEQRKNSGSWTQIAASDSTSKAVHSLTDGSYSYRVRACNASGCSGYRTGSAAT
                     IISLKPGTPAAPSGPSTSTGSVSVSWASVSRATYYDLQKRHNNGSWATAKAGDTNTNE
                     SLSGLTDGSWDYRVRACNTYSWSCSNYSADSSNTTVRLIPSVPAKPTTSTTSTITGSY
                     TVNWTKPSGSVSYYDVQERKNTLSWANLVTGQVTTNAAVSSRTTGNWDYRVRACNGYS
                     WACSGYSADSTDVNVRRVPANTSITKPTSNVSDSGSFMLEWNSASDATLYVLQQQING
                     GSWSTLQSNSSRTYSVSGLTNGTYGYRVKACNGGTDVCSANSVTKTIAVSVTPVYARK
                     SSASVPDAALITPTVPANQAVGALEGQAGVSGGAATYSVPIAIPPGRAGMQPKVSLNY
                     SSRSGNGIAGVGWSLSTGSSIHRCGKTAAQDGFGFGVTYSATEDRLCLDGQRLMVVSG
                     SYGVSGAEYRTELDTFV"
BASE COUNT          450 a          395 c          325 g          405 t
ORIGIN      
        1 acaaaagtat caagttcagt tctatactct gcccctgaaa ctccataact gccagataca
       61 accattagtc gctgaccatc taaacataaa cgatcttcag tcgcgctata ggtgacgcca
      121 aagccaaagc catcttgagc cgcagttttt ccacagcgat gaatggatga accagtactc
      181 aaactccaac caacaccagc aattccatta ccagaacgcg aagaataatt tagcgatact
      241 ttcggctgca tcccagcgcg cccaggagga atcgcgattg gcaccgagta agttgccgca
      301 ccgccagaaa cccctgcttg accttccaat gcaccaaccg cttgattggc tggcacagta
      361 ggtgtaatca gcgcggcatc tggtaccgat gcggatgatt ttcttgcata aacaggtgtc
      421 acagaaacag cgatcgtttt cgtcacagaa ttagccgaac aaacatcggt tccaccgtta
      481 caagctttta ctcgataacc ataggtcccg ttagttaaac cagaaacaga ataagtgcgg
      541 cttgaattag attgcaacgt gctccaggaa ccaccattaa tttgttgctg taaaacataa
      601 agtgttgcat cagaagctga attccattca agcataaaac ttccagaatc acttacattt
      661 gaagtcggtt tggtaattga agtgtttgcc ggtactcgac gtacattaac atcagttgag
      721 tcagcgctgt aaccagaaca ggcccatgaa taaccattac aggcacgaac tcgataatcc
      781 caatttccgg tggtgcgact ggaaactgca gcattagttg tcacttggcc ggtaactaaa
      841 ttagcccaac tcagtgtatt ttttctttcc tgaacatcgt aataactcac actaccggac
      901 ggtttagtcc agtttactgt ataacttcca gtaattgttg acgtggtaga ggttgtcggt
      961 tttgccggca cagagggtat cagccttact gttgtgttcg acgagtcggc agaataattg
     1021 gaacaagacc agctataagt attacaagca cgaactcgat aatcccaact gccgtcagtt
     1081 aagccagaca gagattcatt agtatttgta tcacccgctt tcgctgttgc ccaacttcca
     1141 ttgttgtgac gtttttgtaa atcataataa gtagctcttg atacacttgc ccaactgaca
     1201 gatacgcttc cagtggaagt tgaaggccca gatggtgcag ctggtgtgcc aggttttaag
     1261 ctaattatcg tcgcagcaga acctgttcga tacccactac acccactcgc gttacaagct
     1321 ctaactcggt atgagtaact accgtcagtt aaactatgaa cagccttact cgtggaatcg
     1381 ctagcagcaa tttgcgtcca gctaccacta ttttttcgtt gctccaacct gtaatctgca
     1441 gcacgcccag ttgcgcttcc ccaacttacg gtgatcgaac catcagtatc ggtcgatgga
     1501 ttagaaatac ttgaaggaac acctggcacg tgtaaaacac tgacatcacc actgattgcc
     1561 gaataacaga tcgga
//
LOCUS       DBNHVN010000034         2306 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1640780, whole genome shotgun sequence.
ACCESSION   DBNHVN010000034 DBNHVN010000000
VERSION     DBNHVN010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1640780"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="AC16Y1_00545"
     CDS             complement(<1..1129)
                     /locus_tag="AC16Y1_00545"
                     /inference="COORDINATES: protein motif:HMM:NF015714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR5830986.1"
                     /translation="MLFCVLRLEPLISGPLNQAVRENAVVKVSGTLLTDPIRSNSVSG
                     LDLSTRDFGTFKIKTNRVTYRGNSYQLRVSIQVFVSGENLKKIWQLPPGTQVIAIGKI
                     GNADLLRGVAGNLTATQEIQLISSPPNYQFLASHFRIGLHEVLSFAKPDVAGLIPGLA
                     LGDDSRINPELEADMKTSGLAHLTAVSGSNVTLLISIVLAIGRTFRWTAKTNYSIALF
                     SLLAFVILVRPQPSVLRASVMGLIMVLALFTKSQKSPMPALFASVIMLILIDPWLSVS
                     YGFALSVFATGGLLLWAKSLMMQADQVLPRRIPEWVVAGLVVTLSAQIAVFPLLVSLG
                     SQVSLGSLPANLVSVPLAGPTMVLGLIAAILVPIYFPLAQVF"
     gene            complement(1337..2002)
                     /locus_tag="AC16Y1_00550"
     CDS             complement(1337..2002)
                     /locus_tag="AC16Y1_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR5830987.1"
                     /translation="MFSINLSTHTIQKRLNKLFGEREYKSKFSADNETQFLINPPELA
                     LRPIIRGGAVLTFLLIGFWWLNRPTEIFQIETLNDQTNSVQKLGGGLGEVVVHVTGEV
                     NNPGLVKLPAGSRVMDAITAAGGFTSPESVGNLNLAAHLEDGQLIYVGPDSELPNDPR
                     LNLNTASITQLEALPGVGPVMASRIIDWRAKHNRFSTIDELQEVEGIGPKLFSRLREL
                     VRV"
     gene            complement(2071..>2306)
                     /locus_tag="AC16Y1_00555"
     CDS             complement(2071..>2306)
                     /locus_tag="AC16Y1_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR5830988.1"
                     /translation="HELVRTEVKAINRLVELAMDTNLTGSFLVNHVCAEERANQIADE
                     LKTKSAMSSVPVSEVGAVVAAHVGPGAVAIVIN"
BASE COUNT          749 a          509 c          422 g          626 t
ORIGIN      
        1 caaatacttg agcaagtgga aaatagatcg gaactaaaat cgctgcaatc aaaccaagta
       61 ccatcgttgg tccagccagt ggtaccgaaa ctaaatttgc cggcaaactt cccaatgaca
      121 cctgagaacc caaggaaact agaagtggga atacggcaat ttgtgccgat aacgtgacaa
      181 ctaagcccgc cacgacccat tcaggaattc gacgtggcaa aacttggtca gcttgcatca
      241 ttaaagactt agcccaaagt aataatcctc cggtagcaaa aactgaaagt gcaaaaccgt
      301 aagaaacact cagccaggga tcaatcaaaa tcagcatgat cacacttgca aatagcgctg
      361 gcattggcga tttttgtgac tttgtaaata atgcaagcac cataatcaat cccattacgc
      421 tggcacgcaa cacacttggt tgtggtctaa caaggatcac aaaagcaagc aaactaaata
      481 gcgcaattga ataatttgtt tttgcagtcc agcgaaatgt gcgacctatc gctaaaacaa
      541 ttgaaatcaa tagcgttacg ttacttccag atacggcagt taaatgggct aatccagaag
      601 ttttcatatc tgcttctagt tcagggttaa tacgactatc gtcgccaagt gccaaccctg
      661 gaattagtcc ggcaacatca ggcttggcaa aactcaaaac ttcatgtaga ccaatccgaa
      721 aatgcgaagc tagaaattga taattaggtg gcgatgaaat tagttgaatt tcttgggtcg
      781 ccgttaagtt tcctgcgaca ccacgtaaca ggtctgcatt gccaattttg ccaattgcaa
      841 taacttgagt gccgggaggt agctgccaaa tcttttttaa attttcacca gaaacaaata
      901 cttggattga aactcttaat tggtatgaat ttccacgata agtcacccga ttagttttaa
      961 ttttgaaggt gccaaaatca cgggtgctca aatctagacc gctcactgaa ttactacgta
     1021 ttggatcagt taacaaagtt ccactaactt ttacaactgc attttctctt acagcttgat
     1081 ttagtggccc actaattaat ggctctaagc gcaatacaca aaacaacaca ccaattacca
     1141 ccccaagtac caaaaatttg gaactgacta gttgccagct tttgaatcga aacctaaaaa
     1201 taattaaata acaaaatgaa attaataata aaaaccaaat caaaatcgta aatggatttg
     1261 tagaactagt agtaactaaa gtgccacttg ttgaaagcca cagaccgaga gccaatggta
     1321 aaagtcgtaa gtccaattac actcgaacta attcacgcaa ccgactaaat aactttggac
     1381 ctattccttc aacttcctgt agttcatcaa ttgttgaaaa tcgattgtgt ttcgctcgcc
     1441 aatcaataat tcgacttgcc atgacaggcc ccacacctgg caaagcttca agttgggtga
     1501 tagatgcagt atttaaattg agtcttggat catttggcaa ttcactgtca ggtccaacgt
     1561 agatcaactg cccatcttct aaatgggccg ctaaatttaa atttccaacg ctttcgggcg
     1621 aggtaaaccc acccgcagca gtgatcgcat ccatgacccg actcccagcg ggtaatttca
     1681 ccaaaccagg attatttact tcgcccgtta catgaacaac aacttctcct aagccaccac
     1741 ctaatttctg gacagagtta gtttgatcat taagtgtttc aatttgaaaa atttcagttg
     1801 gtctatttag ccaccaaaag ccaatcaaga gaaaggttag tacggcaccg ccgcgaatta
     1861 ttggtctcag agcaagttct ggtggattaa ttagaaattg agtttcatta tcagcagaaa
     1921 actttgattt gtactctcgc tccccaaata gcttattgag tcgcttttga attgtgtgag
     1981 tggagagatt tattgaaaac atttgactaa attaaaaaaa ttagtccaaa ttaaatcaaa
     2041 taaatattga ttgttaatga attggggttg ttaatttatc acgatcgcga ctgcacccgg
     2101 accgacatgg gccgccacaa ccgccccaac ttcagaaact ggtacggaag acatcgctga
     2161 cttagttttc aattcatcgg caatctgatt tgctctttct tctgcgcaaa catgattcac
     2221 caaaaagcta cccgttaagt ttgtatccat tgctaattca acaagtctgt taatcgcttt
     2281 aacttctgtt cgcactaatt catgta
//
LOCUS       DBNHVN010000035         6953 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1491897, whole genome shotgun sequence.
ACCESSION   DBNHVN010000035 DBNHVN010000000
VERSION     DBNHVN010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6953)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6953)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6953
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1491897"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="AC16Y1_00560"
     CDS             <1..891
                     /locus_tag="AC16Y1_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007444460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HIR5830989.1"
                     /translation="DSVKEINPYVQVNLHELRLDSSNVMDLFAQYDLIVDGTDNFATR
                     YLVNDACVLLNKPYVWGSIYRFDGQASVFWSEHGPCYRCLYPEPPPPGMVPSCAEGGV
                     LGVLCASVGSIQVTEAIKVLTGIGDPLVGSLMVYDALEMTYRKIKVRKDPNCDICGEN
                     PTVTELIDYEAFCGAVSQAAADAIVDSTINVKQLDEMLKARERGEKDFVLIDVREPGE
                     YEIVNIPGAVLIPKNEFLLGDALANLPQDKQIVLHCKSGGRSAEVLAAVKGAGFKDAI
                     HVGGGVLAWVNQIEPGKPTY"
     gene            complement(905..1234)
                     /locus_tag="AC16Y1_00565"
     CDS             complement(905..1234)
                     /locus_tag="AC16Y1_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008868899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HIR5830990.1"
                     /translation="MTTKSITKKSSKNLKPVSPGELLWHEFMEPMSITRYRLAKEINV
                     PAQRIGDIISGKRSITADTDLRLCKFFGLSDGYWLRAQADFDVQIHKSSLEKVLREII
                     PWSESIA"
     gene            complement(1253..1531)
                     /locus_tag="AC16Y1_00570"
     CDS             complement(1253..1531)
                     /locus_tag="AC16Y1_00570"
                     /inference="COORDINATES: protein motif:HMM:NF016875.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR5830991.1"
                     /translation="MIVSFADRETQALSEGKRVPRFESIERSAQRKLIQLRIAQTLAD
                     LAIPPGNNLETLKGNRSGQMSIRINNKWRICFIWTPAKPVEVEIVNYH"
     gene            complement(1582..3957)
                     /locus_tag="AC16Y1_00575"
     CDS             complement(1582..3957)
                     /locus_tag="AC16Y1_00575"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HIR5830992.1"
                     /translation="MSSYSSTLVIEDAVLNRRLRRPGDLARLAVALGLIVGTGLLSYL
                     AQETTTGIGQDISLGANRLPNFLLILASFISGLGVLLFPAATALDLLLRKRARQLLEA
                     LAGLLISIGILTFSTWSLISLDSERFLLAFAGTTDVQSAVPFNILVGGLAAFITVTRT
                     MSRHRWNFVSGVVVGSLALSDIISGGITAAGLFVSLFAGWSVGLLIRYLVGTDTTRPP
                     GQQIADALTGIGHPLSLLRAAEVLQSGRRYDASTLAGNRLDLIVLDRDLEGAGLAQAI
                     YRSIRLRDDSGGGGTSMRRRLERIAMNSWAMSTAGIRTPHLLSVAEVGPDASVLVFDH
                     LPGRSFADFEHKLGDAELAGAWEIVRDLHAARIAHRGLTASSFIQGADGQIYLNNFED
                     GVIAASDVLLRIDLAEALCTLAMRSSVERAITAGRYILGDETLTKALPALQLVALSNE
                     TKNQVKSRKNLLAVLRKSLIEILPEGTEDQEIDIERVKPRTILTITGGSVAAYLLLGQ
                     LTRVNLIDLFTTANAGWVTAALLFSVLTYVAATANMVGVVPERISPWKAFQTQWAASF
                     ATLVAPPTLGSVAVNVRFLVKQGLNSAMAGASIAVIQVMAAIGHVSLLIITAIIAGTS
                     SELAFRPSRTVITWVVIVALLIAGLISIPTVRDWLGRRIKPIVRQVVPRFTSLANQPL
                     KLTSGVVGVVVLNIAYIGALYASVRAFAPDAQLAPVAVVYLAGSIIGQAAPTPGGLGA
                     VEAALAAGLTAAGIDAGVAVSATLLFRLMTFWLPTIPGWLAFRNLQRTGDL"
     gene            4058..>6953
                     /locus_tag="AC16Y1_00580"
     CDS             4058..>6953
                     /locus_tag="AC16Y1_00580"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR5830993.1"
                     /translation="MSLLHGGIDLMMRLILDASRPTPAEPIELDEDQLRVVNHRIGAL
                     RVLAGPGTGKTTTLVEAMAARLIGDQPLKPDEVLGLTFGRRAALDWRDRVTVKVGGGF
                     VPTISTFHSFCYALVRQFQSPEDYLAPFRLLSGPEQEARSRLLFSNSISDGNLTWPTE
                     LMPAVGTRGLAEEVRSVMSRTRSHMMEPADLVKLGSSSQNETWQAIGKFMNEYLDVLD
                     SEGVIDYSELIFRAVLLSNRPDVQKFLHQKFKAIFVDEYQDTDPGQVELLKAMVGPQT
                     SLVVVGDVDQAIYGFRGADEAGIRKFKAEFSPLFNNKFADVVLQNCRRFGPNIRSAAS
                     IVIGDRIPAGFDKADIQKHRTPRVDPTISDELIVRTFDSAGAEAANIAELIARAHAHE
                     NFAWSDIAVIVRSAKVSLPVIQRALVSAGIPVTVAADEIPLHQDPAVMPLLDVLRAVD
                     NPKALTAELSADLLTGPLAGVDQIDLRRLAAHLRKSQRAEGGQVQPSAVLIAQVLAKP
                     GLLADVPPGELSAAVEGITTLGSLIEQARQKMIKGATPHEVLDFIWQATNWKTNLEQR
                     ALSFEFSSHAANRDLDSIGALFDLANRFVSRGGSKDLTIFLSEIESQIIPAESLAEND
                     TRSDSVRLLTAHRAKGLQWPMVVVAGVQEELWPDLRSRQTILQADRIGPKQVLLPPTF
                     AETLASERRLFYVAITRAMQKLVVTAVENSIAGESGTTPSRFITDLRDAKPEINWHHI
                     GGRPKRPLSVEGLIANLRQVLISTEASIELKKAAAIRLNALGQRNIGTFKHALPKNWW
                     GINEVTTNAIPDYISLSATAIDGVEKCSLRWFLEHRAGAVSDNKLNMVFGNILHLIAE
                     GLQNKELPTDIELINKKVDQIWPDQAFNAAWESEFERDQAHAASLRLLNWFNEHSEVE
                     ALAETKLTYASEVTDSEGNRFQLGITGYADRIEFSLDGAIIYDFKTSRKLV"
BASE COUNT         2013 a         1529 c         1550 g         1861 t
ORIGIN      
        1 gatagtgtta aagaaattaa tccttacgtc caggtaaatc tgcacgaact tcggttagat
       61 tccagtaacg taatggactt atttgcccaa tacgacttga ttgttgacgg cacagataac
      121 tttgctaccc gctatttagt caatgatgct tgcgtgcttt taaataagcc ttatgtgtgg
      181 ggatcaattt atcgatttga tggccaagca agtgtgttct ggtctgaaca cggaccgtgc
      241 taccgctgct tatatccaga gccaccacca cctggaatgg ttccctcttg tgctgaaggc
      301 ggtgtccttg gcgtactttg tgcttctgtt ggatctattc aagtcaccga ggcgatcaaa
      361 gttctaactg ggattggtga tccacttgtt ggttcattga tggtttatga cgcacttgaa
      421 atgacttacc gcaagattaa agttcgcaaa gatcccaact gtgacatttg tggtgaaaac
      481 ccaacagtca ccgagttaat cgactacgag gcattctgtg gtgctgtttc acaagctgca
      541 gctgatgcaa tcgtcgattc aacaattaac gttaagcagc tggacgaaat gcttaaggct
      601 cgtgaacgtg gcgaaaagga ttttgtcctt attgatgtgc gcgaaccagg ggagtatgaa
      661 attgtaaata ttcctggtgc agtattgatt ccaaaaaatg aatttttact cggcgacgca
      721 cttgcaaatt tgccgcaaga taagcagatt gtgctgcact gtaaatcagg tggacgttca
      781 gcagaagtac ttgccgcagt aaaaggcgct ggctttaagg acgcaattca cgttggtggc
      841 ggagtgcttg cctgggtgaa tcagatagaa cctggcaaac ctacctacta attaaaaaga
      901 ttgcttatgc aatactttca gaccatggaa taatttcgcg taaaactttt tcaagactcg
      961 acttatgaat ttgcacatca aagtcagctt gagcacgaag ccagtatcca tcgctcaagc
     1021 caaaaaattt acaaagccta aggtcggtat cggcagtgat acttcgcttc ccgctaataa
     1081 tgtctccaat ccgttgagct ggaacattaa tttcttttgc gagacggtat cgggtaatac
     1141 tcataggctc cataaattca tgccacaaaa gttcaccggg ggatacgggc tttaaattct
     1201 tggaagattt ttttgtaatt gatttagttg tcatgttcac ttctcttctc actcagtgat
     1261 aattaacgat ttctacttcg actggcttag ctggggtcca gataaagcaa attcgccatt
     1321 tattgtttat tcgaatactc atctgtcctg accggttgcc tttcaatgtt tctagattgt
     1381 tcccgggtgg gatagcaaga tctgccaagg tttgggcaat tcgcagttgt atcaattttc
     1441 tttgtgcact cctctcgatt gactcaaatc ggggaactcg ttttccttcg gacaaggctt
     1501 gagtttcacg atcggcaaaa gaaacgatca tttggtaata ttaacgcgta acgttattaa
     1561 tgtaaagcgt tattattatg gctaaagatc gccggttcgc tgcagatttc taaacgccaa
     1621 ccaaccagga atagttggca accaaaaagt cattaagcga aatagcagtg tcgccgaaac
     1681 tgcaacacca gcatcaattc cagctgcagt taaacctgct gctagagcgg cttcaacagc
     1741 tcctaaacca cccggcgtag gagcggcctg accaataatc gagccagcca aataaacaac
     1801 agcaactggt gccagctgag catcgggtgc aaatgcccga acggaagcat aaagtgcacc
     1861 gatgtaagcg atgttaagca caacgacgcc aactacgcca gaagttaatt taagcggctg
     1921 gtttgcaagt gaagtaaacc ttggcacaac ttgacgcaca atcggtttaa ttcttcttcc
     1981 taaccagtcg cgcacagttg gaattgatat caacccggca atcagaaggg caactatcac
     2041 aacccaggta atcactgttc gagatggacg gaatgcaagt tcgctagaag taccggcaat
     2101 tattgcagtt atgatcagca gactgacgtg cccaatcgct gccataactt ggatcaccgc
     2161 aatacttgct ccggccatcg ctgaatttaa accttgttta actaaaaagc gcacattaac
     2221 ggcaactgaa cccaaggtag gtggcgccac taacgttgca aatgaagcag cccattgagt
     2281 ttggaatgct ttccaaggtg aaattcgctc aggaactaca cccaccatat ttgccgtagc
     2341 tgctacataa gtaagtaccg agaacaaaag tgctgcggtt acccagccag cattagccgt
     2401 agtgaataag tcgattaaat taacccgagt tagttgaccc aatagtaggt aagccgcgac
     2461 actaccgcca gtaattgtca gaatcgttcg aggcttaacg cgttcaatgt ctatttcttg
     2521 atcttcagta ccttcaggaa gtatttcgat aagtgactta cgtaagaccg caagtaaatt
     2581 cttgcgagat ttgacttgat tcttagtctc attagaaagt gcgactaatt gaagtgccgg
     2641 aagggcttta gttaatgttt catctcccaa aatgtagcga cctgcggtga ttgctcgctc
     2701 tacagaactg cgcatagcta gcgtgcaaag tgcttcagct aaatcaatcc gtagtagcac
     2761 gtcactagct gcgataacac catcttcaaa attgtttaaa tagatttgcc catcggcacc
     2821 ttgaataaaa ctgctggcgg ttaaaccacg atgggctatt cgagcagcgt gtaagtcacg
     2881 cactatttcc caagcaccag ctaattctgc atcacctagt ttgtgttcaa agtcagcaaa
     2941 acttcgacct ggtagatgat caaaaactag aacagatgcg tcaggtccaa cttcagccac
     3001 tgaaagtaga tgcggagtac gaattcccgc cgtggacatt gcccaactat tcattgcaat
     3061 gcgttcaagg cgccggcgca tcgaagtgcc accgccacct gaatcatccc gtaaccgaat
     3121 tgatcgataa attgcttgag ctaagccagc accttctaaa tcgcgatcta aaacgattaa
     3181 gtccaaccga tttccagcta aagtactggc gtcataacgt cggcctgatt gcaagacttc
     3241 ggcagcgcgc aaaagggaaa gtggatgtcc aatcccggta agtgcatcgg caatttgttg
     3301 acctggcggc cgagttgtat ctgtgccaac tagataccgg attaacaggc caactgacca
     3361 accagcaaaa agtgaaacaa ataaaccagc tgcagtaatt ccaccgctaa taatgtctga
     3421 gagtgcaagt gagccaacta cgacaccaga tacgaagttc catcgatgac gagacatagt
     3481 ccgcgttact gtaataaacg cagctaagcc accaaccaaa atattgaatg gcactgcgga
     3541 ctgaacatca gtagttccgg caaaggcaag taaaaatctt tctgaatcca aactgatgag
     3601 cgaccaagta gaaaaagtga gtatgccgat tgaaattaac aaaccggcta aggcttcaag
     3661 taattgccgt gcacgtttgc gaagtaacag gtcaagcgcg gttgctgctg gaaaaagtaa
     3721 aacacccaag ccagaaataa agcttgccaa aatcagcaaa aagtttggta accgattggc
     3781 accaagtgaa atatcttggc caattccagt tgtagtttct tgcgctaggt aactaagtag
     3841 tcccgtgccc acaattagac caagtgccac agcaaggcga gccaggtcac ctggacggcg
     3901 aagtcggcgg ttaagcaccg cgtcttcgat taccagtgtt gaactataag aactcaaacc
     3961 cgtttacctt gtatttagcg ccgcagctca cccaaagact gcaaaacgat tattttccta
     4021 ttatctcgaa gttttatgcg ctttctgtgc gggcaaaatg tcgttactgc atggtggaat
     4081 agacctaatg atgcgcctga tccttgatgc ctcgcgacca accccagccg agccgattga
     4141 actagatgaa gatcaacttc gtgttgttaa tcatcgaatt ggcgcattac gagttttagc
     4201 aggtccaggc accggcaaaa ctacgacttt ggtcgaggcc atggctgcca ggttgattgg
     4261 cgatcagcca cttaaaccag atgaggtact cggcttaacg tttggtcgtc gagccgcact
     4321 tgattggcgt gaccgagtaa ctgtgaaagt tggtggcgga tttgttccaa caatcagcac
     4381 cttccattcg ttttgctatg cattagtgcg gcaatttcaa tcacccgaag attacttagc
     4441 tccatttcga ttactttccg gtccagaaca agaagcacga tcgcgtcttt tattcagtaa
     4501 ctcaatatca gatggcaact taacttggcc gactgaactt atgcctgccg taggcacgcg
     4561 cgggttagct gaagaagttc gttcagtcat gtctcgaact cgttcgcaca tgatggaacc
     4621 agctgactta gtcaaactcg gatcaagttc acaaaatgaa acttggcagg caatcggcaa
     4681 atttatgaat gagtatcttg acgtactcga ttctgaaggc gtcattgact acagtgaatt
     4741 aatttttaga gctgtgcttt tgagtaatcg accggatgtc caaaaatttt tacatcaaaa
     4801 atttaaagcg atttttgttg atgaatacca agatacagat cctggccaag ttgaattatt
     4861 gaaagcaatg gttggtccac aaacatcgct agttgtagtt ggagatgtcg accaggcaat
     4921 ttatggattc cgtggcgccg atgaagccgg aattagaaaa ttcaaagctg aattcagccc
     4981 actttttaat aataaatttg cagatgtagt tttacaaaat tgtcgtcggt ttggtccaaa
     5041 cattcgaagt gcggcttcga ttgtgattgg cgatcgtatt ccagcgggct ttgataaagc
     5101 agacattcaa aaacatcgaa cgccgcgagt tgatccaacc attagtgatg aattaattgt
     5161 tcgtacattt gattcagctg gggccgaagc tgcaaacatt gctgaactta ttgcgcgagc
     5221 tcatgctcat gaaaattttg cctggtctga tatagcagtg atagttcgct cagccaaagt
     5281 ttctcttcca gttattcagc gagccttagt aagcgcgggt atcccagtaa ctgtggccgc
     5341 agatgaaata ccgcttcacc aagatccagc agttatgcct ttacttgatg tgttgcgcgc
     5401 cgttgataat ccaaaagcgt taaccgctga attatctgca gatttactaa ctggaccact
     5461 tgccggtgtg gaccaaattg atttgcgcag gttagctgct catttgcgta aatctcaacg
     5521 agctgaaggt ggacaagtcc aaccatctgc agttttaatc gcccaagtac ttgccaagcc
     5581 aggattactt gctgatgtgc caccaggtga gttgagtgca gccgttgaag gcataactac
     5641 tctaggcagt ttaattgaac aagctcgtca aaagatgatt aaaggtgcta cacctcatga
     5701 agttttagat tttatttggc aagccacaaa ttggaaaaca aatttagagc aacgagcact
     5761 ttcttttgaa tttagctcgc atgcagctaa tcgtgatcta gattcaattg gcgcactttt
     5821 tgatttagca aatcgttttg tttcaagagg tggcagtaaa gatttaacaa tttttctttc
     5881 tgaaattgag tcacagatta tcccggccga atcacttgcc gagaatgaca ctcgaagtga
     5941 ttctgttagg ctactaactg cacatcgggc taaaggtttg caatggccaa tggttgtagt
     6001 tgcaggtgtt caagaagaac tttggcctga ccttcgctct agacaaacta ttttgcaggc
     6061 tgaccgaatt gggccaaagc aagtacttct gccacctact tttgctgaaa ctttagctag
     6121 tgaacgccgg cttttttatg tggcaataac tcgagcaatg caaaagttag ttgtaactgc
     6181 cgtcgaaaat tccatcgcag gtgaatctgg aactacacca tcaaggttta ttactgactt
     6241 aagagatgca aaacctgaga ttaattggca ccacataggc ggacggccta agagaccgtt
     6301 aagtgttgag ggtttaattg caaatctgcg tcaagtattg atttctactg aggcctctat
     6361 tgaactcaaa aaagctgcgg caattcgatt aaatgcactt ggtcaacgca atattggaac
     6421 atttaagcat gccttgccaa aaaactggtg ggggatcaac gaagtaacaa cgaatgcaat
     6481 tcctgattac atatcacttt cagcaacagc aattgatggt gttgaaaagt gttcgttacg
     6541 ttggtttctt gaacatcgag cgggcgcagt ttcagacaat aaattaaata tggtctttgg
     6601 aaatatcctg cacttaattg cagaaggctt acaaaataaa gaactaccaa ccgacattga
     6661 gttaattaac aaaaaagtcg atcaaatctg gccggaccaa gcatttaatg ctgcttggga
     6721 atctgagttt gagcgtgatc aagcgcatgc agcttcactt cgactcctta actggtttaa
     6781 cgagcacagt gaggttgaag cattagctga aactaaatta acttatgcca gcgaagtaac
     6841 agatagcgaa ggtaaccgat tccagttagg aatcactggc tatgcggacc gaattgaatt
     6901 cagtttagat ggcgcaataa tttatgattt caaaacttca cgcaaattag tta
//
LOCUS       DBNHVN010000036         6469 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_229315, whole genome shotgun sequence.
ACCESSION   DBNHVN010000036 DBNHVN010000000
VERSION     DBNHVN010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6469)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_229315"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="AC16Y1_00585"
     CDS             complement(<1..374)
                     /locus_tag="AC16Y1_00585"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR5830994.1"
                     /translation="MSSVVSLEVSRLAFAYADGHQALFGVDFQIFRGERVALLGPNGA
                     GKTTLVMHLNGILSPGHGYVKVNDQIVDSQDKTGLKQIRTKVGIVFQDPDDQLFMPTV
                     REDVAFGPKNMGVQGEQLEQAV"
     gene            complement(376..1176)
                     /gene="cbiQ"
                     /locus_tag="AC16Y1_00590"
     CDS             complement(376..1176)
                     /gene="cbiQ"
                     /locus_tag="AC16Y1_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HIR5830995.1"
                     /translation="MIQERITLTQSGHEHGHDLIDHLYIHEHTRIHNLPGHLKVMGAL
                     LFIFVVVATPSREYLAFLLYFLGLLLIARLAKLPYRKLAKRMLIEIPFVFFAVLIPFA
                     TPGEKIVVLGLSVSPEGLWVAFGILAKSTLGVMTSIILSATTQSRELLVSFRKLKVPD
                     TLIQIASFMFRYTAVVIDELNRMKVARQSRGFEARGVKDWRILGQSIGALFVRAYERG
                     ERVHLAMLARGYDGNLLGDNLPNSKSSEKFIAFSPALFALIVQLIVRN"
     gene            complement(1173..1463)
                     /locus_tag="AC16Y1_00595"
     CDS             complement(1173..1463)
                     /locus_tag="AC16Y1_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024588.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HIR5830996.1"
                     /translation="MSIKRFVSLAILISVLVAGILSYFASSNPDGLEKVSEDKGLDVQ
                     VTDSAVADSIFADYGIAGLENSTGLAGLVGLIITGGIGYLLIKWVSKSSSKN"
     gene            complement(1460..2152)
                     /locus_tag="AC16Y1_00600"
     CDS             complement(1460..2152)
                     /locus_tag="AC16Y1_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HIR5830997.1"
                     /translation="MHIPDGFFDAPTSIGAAVIAAGAVGASLKLSKGKMESEVAPVAG
                     LTAVFIFAAQMINFPVALGTSGHLIGGALAAILIGPWAAMLAMTVVLAVQALVFADGG
                     LTALGLNIVNMGIITVLVGYSIFNLAMRILPKKRIGILIAAGLAAFISVPASSTGFVI
                     QYAIGGTATFELQDVFLAVTGVHLLIGLGEALITVLVVSSVLAARADLVTSARNYLPK
                     AKLVIRNQDVNV"
     gene            2271..2525
                     /locus_tag="AC16Y1_00605"
     CDS             2271..2525
                     /locus_tag="AC16Y1_00605"
                     /inference="COORDINATES: protein motif:HMM:NF023697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HIR5830998.1"
                     /translation="MSEQKKPKDQVERISQELADARSSLTESVEQLGDYLKPNKVASR
                     SMQKVTDFFVDENGQVKPERLAIAGAAVLGLIGLLTRDRD"
     gene            complement(2567..3181)
                     /locus_tag="AC16Y1_00610"
     CDS             complement(2567..3181)
                     /locus_tag="AC16Y1_00610"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013169999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HIR5830999.1"
                     /translation="MAELVFFTGTMDCGKSTLAAQVNYTHASRGRRGMVFTCNDRAGS
                     GVLSSRLGLEVASTEVTPETNFFDIAIALHKTTDQLDYLICDEAQFYSRAQIDQLAHV
                     VDDLDIDVFAFGLSADFRTELFPGSARLFELADRIQVLQVEALCWCGKKATHNARTIN
                     GVMVTEGEQVVVGDTASEDLVAYEVLCRRHHMAAMPLGAASAED"
     gene            complement(3197..3796)
                     /locus_tag="AC16Y1_00615"
     CDS             complement(3197..3796)
                     /locus_tag="AC16Y1_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HIR5831000.1"
                     /translation="MSNQIETQLEVQLENRNRLTALFRPLLVIPAYIFLTAFSNWASE
                     ENEIATWTIGFLFFPTLLAIVFRGRYPSYALAFNQALLDLSLRFTSYLFLLTDKYPSI
                     EGNETVKTTFPDVQGGRALHRGLPLVKWFLAIPLYLLGFVYALYAGLFVVFGWVSIVF
                     TGTLPQKAADIICKVIAYWNRVTGYTFLLVTDEYPTFKL"
     gene            3858..5291
                     /locus_tag="AC16Y1_00620"
     CDS             3858..5291
                     /locus_tag="AC16Y1_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013357.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HIR5831001.1"
                     /translation="MHNSHRINSRFVPKKVIIALLSIALVVFALPQAASANDAITAAR
                     AELAQTMKALDTAKTNLAKAEKELANAKSIAAKIDKELKDAEASLNQVQAEMDLLNAQ
                     IAEDQAQLDEIVRTSYKMGVSREWLIVDVIISSDTDENITSKLELLNIVLDSSNDVLA
                     LLLEKKTELDIKVQEAEEIKADIQVKNEEAKKLVVELAEKTKLAQDEANRVQALANEK
                     EAVLRKLVFAGLPASGTVLGADISYVQHPGGAPIDFVRMYDAGVRFLYIKGSSGGDGP
                     NAQALRLASEDLPKAREAGILTGIYHVALIAAGSSVESAAAQGAAQANRAVSNMNAMG
                     GFKPGVLPIALDVEGFSLAGGAGTPASVVSAFSVSFANTVRAQTGRTTVIYSNLSFLK
                     QYLTDPALKNYPLWVANLTSGSNPGSLTNGTCLPTVWTSPGCSLNWTFWQYTHTAPAA
                     NYGIARGYLDLNRLGKSINELLALAGY"
     gene            5341..5994
                     /gene="trhA"
                     /locus_tag="AC16Y1_00625"
     CDS             5341..5994
                     /gene="trhA"
                     /locus_tag="AC16Y1_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HIR5831002.1"
                     /translation="MIDISKPKLRGIIHLVMSPISLVAGLVMISAASELRGRVTLGIF
                     TFTAVTLFTCSALYHRVKWTDKYRGLWRRIDHANIPILIAGTYTPFSVFLLADPARTY
                     LLVAIWVAALSTAFLRIVWLRAPRWLYVVAYLLLGWAALAFVPTFSRNGGVVVFSLIL
                     LGGIFYTAGGIVYALKKPNFNQTWFGFHELFHALTAAAFVSHFIGAIFTVFGITVDR"
     gene            complement(6154..6227)
                     /locus_tag="AC16Y1_00630"
     tRNA            complement(6154..6227)
                     /locus_tag="AC16Y1_00630"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6191..6193),aa:Met,seq:cat)
     gene            complement(6295..>6469)
                     /locus_tag="AC16Y1_00635"
     CDS             complement(6295..>6469)
                     /locus_tag="AC16Y1_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HIR5831003.1"
                     /translation="DFFKSVKVNPKAKQITGTICGVRIEEIEDPLMKKIRYMDKLVDE
                     LAKGRPMEKVLRS"
BASE COUNT         1917 a         1458 c         1337 g         1757 t
ORIGIN      
        1 ttcactgctt gctctagctg ctcaccctgg acacccatat tctttggacc aaatgcaaca
       61 tcttcgcgaa cagtgggcat aaatagttga tcatctggat cttgaaaaac aatgccaact
      121 ttagttcgga tttgttttag cccagtttta tcctggctat caacaatttg gtcattaact
      181 tttacgtaac catgccctgg actcaagatt ccgtttaagt gcatcactaa agtagttttt
      241 ccagcaccgt taggcccaag cagagcaacc cgctcaccgc gaaatatctg aaaatcaaca
      301 ccaaagagag cttgatgtcc atctgcgtaa gcaaaagcta atcgacttac ttctaggctg
      361 acgaccgaac tcaacttagt tcctaactat tagttgcaca atgagtgcaa aaagggccgg
      421 gctgaacgca ataaattttt cgctactttt ggaatttggc aaattatcac caagtaaatt
      481 cccgtcgtaa cctcgagcca gcatggccaa gtgaacccgc tcgcctcgct cataagcccg
      541 aacaaataag gctccaatag attgaccaag tattcgccaa tcttttacgc ctcgagcctc
      601 aaagccacgg gattgcctgg caactttcat tcgatttaac tcatcgatta ccacggctgt
      661 atatctgaac atgaaagagg cgatttggat caaagtgtct ggaactttaa gttttctaaa
      721 gctaactaat aactctctac tttgagttgt tgcggacaag ataatcgaag tcattacacc
      781 cagggtactt tttgccagaa ttccaaatgc gacccaaagt ccttcgggcg aaaccgaaag
      841 tcctaaaaca actatttttt ctccgggtgt ggcaaaagga attaaaaccg caaaaaagac
      901 aaatggaatt tcaatcaaca tccgcttggc taattttcga taaggaagtt tagctagtct
      961 ggctatcagc aatagaccca gaaagtaaag caaaaaagcc aggtactcac gagatggagt
     1021 tgcaactact acgaaaataa ataacagagc gcccataacc ttcagatggc caggcagatt
     1081 gtgaattcga gtgtgctcgt gaatgtatag gtgatcgatc agatcgtgac catgctcatg
     1141 acctgattgc gttaaagtaa tgcgttcttg aatcaatttt tacttgaact ttttgatacc
     1201 catttgatta acaaataacc aatgccacct gtgataatta agccaacaag accagccaaa
     1261 ccggtgctat tttctagccc agcaattcca tagtcagcga aaattgagtc agccaccgcg
     1321 gagtcagtta cttgtacatc gaggccttta tcttcagaaa ctttttctaa tccatctgga
     1381 ttagaacttg caaagtaact taaaatacca gcgaccaaaa ccgatattaa aatagcgagc
     1441 gatacaaagc gcttaatact catacgttta catcctgatt tcgaataacc aattttgcct
     1501 ttggcaaata gttgcgggca ctcgttacta aatcagcacg agcggccaaa accgaactaa
     1561 cgactaaaac tgtaattaaa gcctcaccta aaccgataag caagtgcacg ccagttactg
     1621 ctaaaaatac atcttgtaat tcaaaggttg cagtgccacc tattgcatat tgaattacaa
     1681 agccagttga ggatgctggg acggaaatga atgcagccaa tccggcagca ataagaatgc
     1741 caatcctttt cttgggcaaa attcgcattg ccaagttaaa aattgaataa cccactaaaa
     1801 ctgtaattat gcccatgttt acgatattta agcccaatgc agttaaccca ccatcagcaa
     1861 aaactagagc ttgaactgca aggaccacgg tcattgccaa cattgccgcc catgggccta
     1921 tcaaaatcgc agcaagcgcc ccaccaatta aatgaccact ggtacccaag gcgactggaa
     1981 aattaatcat ctgagctgca aatataaaca cagcagttaa gccagcaacc ggagctactt
     2041 cgctctccat cttgcccttg ctgagcttta gcgaagcacc taccgcacca gcagcaataa
     2101 ccgcagcccc aattgaagtt ggggcgtcaa aaaaaccatc cgggatatgc acttatttac
     2161 tccaaattct tattgcaaac tctttgcaat aagaataacc cacatttgag aatttttccc
     2221 cacgggattt agcggtttat tcaaggatta attctttgta ggctggttat atgtcagagc
     2281 aaaagaagcc aaaagaccag gttgagcgta tttctcaaga actagctgat gcaagaagtt
     2341 ccctgaccga atcggttgag cagctcggag attatctgaa gccgaataaa gttgcatcga
     2401 ggtccatgca aaaagtgact gattttttcg tagacgaaaa tggtcaagtt aaacctgagc
     2461 ggttagctat tgcgggcgca gcggtgcttg ggttaattgg cctactaact cgcgaccgcg
     2521 actaaatttt cagtcctcgg ctgttcttat tccagcctcg atcctttcag tcctcggctg
     2581 atgcagcgcc caacggcatt gccgccatat gatgtcgccg acacaaaact tcgtaagcca
     2641 ccagatcctc agatgcagta tcgccaacca caacctgctc accttcggtg accatcacgc
     2701 catttatagt gcgcgcatta tgagttgctt tttttccaca ccaacaaagt gcctcaactt
     2761 gaagcacctg aattcgatcg gctaattcaa aaagtctggc tgagcctgga aaaagttcag
     2821 ttcgaaaatc tgctgataaa ccaaatgcaa aaacatcaat gtccaaatca tcaacgacgt
     2881 gagctaattg atcaatctgg gcccgcgaat aaaactgtgc ctcatcacaa attaaataat
     2941 caagctggtc tgtcgtttta tgtaaagcaa ttgcgatatc gaaaaagttt gtctctggcg
     3001 ttacttcagt tgaggcaact tctaagccaa ggcgactaga aagcacgcca cttccagcac
     3061 gatcgttaca agtaaagacc attcctcgac gacctctact tgcgtgagtg taattaactt
     3121 gtgcggctag tgttgattta ccgcaatcca ttgttccggt aaaaaatacg agttcagcca
     3181 tttaacttac agactcctaa agtttgaaag tagggtactc gtcggttact agcaggaatg
     3241 tgtagccggt tactcggttc cagtaagcaa ttactttaca aataatgtct gcagcttttt
     3301 gcggcaaagt gcccgtaaat acgatactca cccagccaaa aactacaaat aatccagcgt
     3361 aaagtgcata gacaaaacct agtaggtata gcggaattgc taggaaccac ttgaccagag
     3421 gaagccccct gtgcaaagcc cgcccacctt gcacatcagg gaatgtggtt ttcacagttt
     3481 cattaccttc aatacttggg tacttgtcag taagaaggaa taagtaagaa gtgaatcgca
     3541 gacttagatc taatagggcc tggttgaaag ccaacgcgta tgacgggtat ctgcctcgaa
     3601 aaactatggc gagcaaagtt gggaaaaaaa gaaatccaat agtccaagtt gcaatctcat
     3661 tttcttctga tgcccaattc gaaaatgcgg ttaaaaagat atacgccggt ataactagta
     3721 atggacgaaa tagcgcagtc aagcgatttc gattttctaa ttgaacttct aactgagttt
     3781 caatttgatt gctcattgcg acagtccggc ttccttgtgt tcaaaggggt ttctgctcaa
     3841 taggttacaa tcaaaatatg cacaatagcc accgtattaa tagtcgcttt gtgcctaaaa
     3901 aggtcataat tgccctgctt tccatagcat tagttgtttt tgcgctccca caggctgcct
     3961 ctgcaaatga tgcaattact gctgcccgtg ctgaacttgc ccaaaccatg aaagcccttg
     4021 atacggcaaa aactaatcta gctaaagccg aaaaagaact tgctaacgca aaatctatag
     4081 cggccaaaat tgataaagaa ttaaaagatg cagaggcttc gcttaaccaa gtccaagcgg
     4141 aaatggatct acttaacgca caaattgcag aggatcaagc ccagttagat gagatcgtca
     4201 gaactagtta caagatgggc gttagccgtg agtggttaat tgttgatgta attatcagtt
     4261 cagatactga tgagaatata accagcaaac ttgaattgct gaacattgtt ttggatagta
     4321 gtaacgatgt tcttgccctt cttcttgaga aaaaaactga acttgacatc aaagttcaag
     4381 aagccgaaga aatcaaagct gatattcaag taaaaaatga agaagccaaa aaacttgttg
     4441 ttgagctagc cgaaaaaact aagttagccc aagacgaggc aaatcgcgtt caagcattag
     4501 caaatgaaaa agaagccgtc cttagaaagt tagtttttgc tgggcttccg gcttcgggaa
     4561 ctgttctagg tgcagatatt tcatatgtgc aacatccggg cggtgcgcca attgattttg
     4621 ttcggatgta cgacgcagga gtccgctttc tttatataaa gggttctagt ggtggtgatg
     4681 ggccgaatgc ccaagcgttg cgcttagcga gtgaagactt accaaaggca cgtgaagcag
     4741 gaattcttac tggcatttat cacgtagcgt taatcgcggc tggaagtagc gttgaaagtg
     4801 ctgcggcaca aggagcagcg caagcaaatc gggcagttag caacatgaat gcaatgggcg
     4861 gatttaaacc tggcgtactt ccaattgcgc ttgatgttga agggtttagt ttggcaggtg
     4921 gagcaggaac tccagcttct gtcgtatctg cattcagcgt ttcatttgca aatactgttc
     4981 gagcccaaac tggacgtaca acagttatct actcaaactt aagtttctta aaacagtacc
     5041 taactgatcc cgctctgaaa aattatccgt tatgggtggc aaatctaact tctggatcaa
     5101 atcctggctc acttacgaac ggaacctgct tgccaacagt ttggacaagt ccaggttgtt
     5161 cacttaactg gactttttgg cagtacacac atactgcacc agcagctaat tacggtattg
     5221 ctagaggtta cctagattta aatcgtcttg gtaagtcaat aaacgagtta cttgctcttg
     5281 ctggttacta aatagttttt gcaatctttc accgaattta aatacgcgta caattaaatt
     5341 gtgatagata tttctaaacc aaagttgcgc ggcattattc atttagttat gtcgccaatt
     5401 tcactagttg ctggtttagt gatgatttca gctgcatcgg aattacgcgg gcgagtaact
     5461 ttggggattt tcacattcac tgccgtcacc ctattcacct gtagtgcgct ttaccaccgg
     5521 gtgaagtgga cagataagta tcgaggactt tggcgccgaa ttgaccatgc aaatattccg
     5581 attttgatag ccggaactta cacaccattt tcagttttct tgcttgcaga tccagccaga
     5641 acgtatctct tagtcgctat ctgggtagct gcactgtcaa ctgcatttct tcgaattgtt
     5701 tggttacgcg caccacgttg gctttatgtc gtcgcatatt tattacttgg ttgggcagct
     5761 ttggctttcg tcccaacctt ttctcgaaac ggcggggtag ttgtgttttc gttaattctt
     5821 ttgggcggaa ttttctatac cgccggtgga attgtttatg ctttgaaaaa acctaatttc
     5881 aatcagactt ggtttggatt tcatgaatta ttccatgcgc ttaccgcagc agcatttgtt
     5941 agccatttca ttggcgcgat tttcactgtt tttgggatca cggtagatag gtagaaatta
     6001 aaaaaatccc aggagttcca tcctgggatt tatcttttag tagcggggac aggaaccaaa
     6061 agattgcccc cggcaatctt tctcccaaat cctggctcgg ctaaaactta aaaaacaaac
     6121 ccgtggagga tcaccccacg ggtttgtttt tagtagcggg gacaggattt gaacctgcga
     6181 cctctgggtt atgagcccag cgagctaccg agctgctcca ccccgcgtcg gctcatcaag
     6241 gatacgacag ttagttgagc aaacaaaatc gactaaaagc caaaaagtaa agatctaact
     6301 tctaagtact ttttccattg gtcgaccttt ggctaattca tcaaccaact tgtccatgta
     6361 tcgaattttc ttcatcagcg ggtcttcgat ttcttcaatt cttacaccgc aaatagttcc
     6421 agtgatttgc ttggctttcg ggttaacttt cactgattta aagaagtct
//
LOCUS       DBNHVN010000037         3354 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_452407, whole genome shotgun sequence.
ACCESSION   DBNHVN010000037 DBNHVN010000000
VERSION     DBNHVN010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_452407"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="AC16Y1_00640"
     CDS             complement(<1..498)
                     /locus_tag="AC16Y1_00640"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF015534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HIR5831004.1"
                     /translation="MVNRYIVGSSGGFNQTERWGVVTPGNIFLRALELTGKTRPRVLF
                     VLTASGDDKNYLATMYQAVSQLSVDAEHLALFTQPNVPVEEAFGRADLVWVGGGSVAN
                     LLSVWRVHGVDQAAKDAWERGVVLGGVSAGSICWHVGGPTDSFGPKLETVTNALGLLP
                     WGNGVH"
     gene            complement(512..2704)
                     /locus_tag="AC16Y1_00645"
     CDS             complement(512..2704)
                     /locus_tag="AC16Y1_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR5831005.1"
                     /translation="MAKHGRRQKTEKTARKALWFAILVIVAWLGIGGTTGPLFGKLTS
                     VQENDSAGFLPGSAESTKAGEISTKFVTEDSSVLPAIIIFTGEINPNAIANLTTFVGT
                     LPNAEVPDSGGKTIGDFLADGTPLFPIPSQDNKAAIISIPIDDSVIAQPLENNEPALP
                     VLVETIRAQANAVDGFESHVTGIGGILADLFGSFGDIDTLLLLKTLGIVAAILIFVYR
                     SPVLWIIPLLCAVLALSVGGGLVYLLASNDIIKVDGQSQSILSVLVIGAATDYALLLI
                     ARYREELHHFESRTAAMRVTLRGVFEPILASGLTVISGLLVLSLSDLAQVRGLGPVSA
                     IGILAAQLVMLTLLPAILILFGRWIFWPRIPRHDDQDEKLTGIWSRVANTVGTHPRRT
                     WVSTAILLSIAFAFVTQLNTRGLATTDSFIGNPDSVVGLEKLSEHFPAGQGDPTLVIV
                     PKDDMQKVTDVLLGTDGVSGVTPTLTGPTIVGAPAPEVKYVDGFARLEVTLDKAADSV
                     EAQDYIPTIRQVAKEASPEALVGGSSAINYDLQQANQRDRNLIIPIALVVIGSMLALL
                     LRSLLAPLLLIATTVLSFGATLGISHLVFTYLFDFPGTDGSFPLFAFVFLVALGIDYN
                     IFLMTRIREEAIKLGTREGILKGLTVTGGVITSAGVVLAATFAVLGTLPLVFIVQLGF
                     AVAFGVLLDTIIVRSLLVPALSHDIGAKIWWPSKLGKTEGPLELVVKS"
     gene            2824..>3354
                     /gene="lipB"
                     /locus_tag="AC16Y1_00650"
     CDS             2824..>3354
                     /gene="lipB"
                     /locus_tag="AC16Y1_00650"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018826693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR5831006.1"
                     /translation="MPVLIDSSTLGKVFHNYEEMWQYQQKVHESVVAGSSPDTILLLE
                     HEPVYTAGRRTEIFERPTDGTPVVDVDRGGKITWHGPGQLVGYPIVHLPAALDVIPHV
                     RRLEEVLIKTCAEFELTTTRIKGRSGVWVTEGGLDRKVAAIGVRVAQGVSMHGFALNC
                     NNDLDWFDHIVPCGISD"
BASE COUNT          964 a          770 c          757 g          863 t
ORIGIN      
        1 gtgcacgcca tttccccaag gcaataaacc aagtgcattt gtaacggtct caagttttgg
       61 tccaaatgaa tcagtcggtc ccccaacatg ccagcatatt gatccggcac taactccgcc
      121 aagaacaacc ccgcgctccc aagcgtcttt ggctgcctga tcaacgccgt gcactcgcca
      181 gacggaaagt aaattcgcta ctgatccacc gccgacccaa accaagtcag ctcgcccaaa
      241 agcttcttca acaggaacat ttggctgagt gaacaaggca agatgttcgg catcaactga
      301 tagctgagac acggcttgat acatagtcgc tagatagttt ttatcatcgc cacttgccgt
      361 taatacaaat aaaacccggg gtcgcgtttt accggtcagc tcaagtgccc gtaaaaaaat
      421 atttcctggc gtaactactc cccagcgttc ggtttggtta aatccgccgc tcgagccaac
      481 tatgtagcga ttgaccatga ctacctttta tttaactttt taccacgagc tctagtgggc
      541 cttcagtttt gccgagttta cttggccacc aaatcttggc gccgatgtca tgagatagcg
      601 ctggaactag caaactgcgc acaatgatgg tgtcgagtaa cacaccaaac gccactgcga
      661 aaccaagttg cacaataaat acaagcggga gtgtgccaag aactgcgaat gtcgcagcaa
      721 gtacaacacc tgcagaagta ataacaccgc cagtaacggt taggcccttc aaaataccct
      781 cgcgggtacc taatttgatt gcttcttctc gaattcgagt cattaagaag atgttgtaat
      841 caatacctaa tgcaactaaa aatacgaaag caaacaacgg gaatgatcca tctgtacctg
      901 ggaaatcgaa gagatatgta aacacaaggt gtgaaatacc gagcgtggca ccgaacgaca
      961 gaactgtagt tgcaatcaaa agaagtggcg caagaagact gcgaagcaac agcgccaaca
     1021 tacttccgat tacgactaac gcgatcggga taattaagtt tcgatctcgt tgatttgctt
     1081 gttgtaagtc atagttaatt gcagatgaac cacccacaag cgcctctgga ctagcttctt
     1141 ttgcaacttg cctaatcgtt ggaatgtaat cttgagcttc gactgaatct gctgctttat
     1201 ctaatgtgac ttctagtcga gcaaagccat ctacatactt cacttcggga gccggtgcac
     1261 caacgatggt gggaccagtg agtgtcggag ttacgcccga tacgccatca gtgcccagaa
     1321 gtacatcagt aactttttgc atatcgtctt taggcacgat tactagagtt gggtcacctt
     1381 gaccagcagg aaaatgttca gataattttt caagacctac aacagaatca ggatttccga
     1441 tgaatgaatc tgtagttgct agccctcgag tatttaattg agtaacgaaa gcgaaggcaa
     1501 ttgaaagcag gattgcagtt gatacccaag tgcgacgtgg atgtgtgcca actgtgttag
     1561 ctactcgaga ccaaatgcca gttaactttt catcttgatc atcatgtctt ggaattcttg
     1621 gccaaaaaat ccagcggcca aacaagatca agatcgctgg taacagtgtc agcatcacaa
     1681 gttgggcagc caaaatacct attgctgaaa ctggtcctag tccacgaact tgtgctaaat
     1741 cagataacga aagaactagt aagccggaga taactgttaa tcctgaagcc agaattggtt
     1801 caaaaactcc acgtaaagtt accctcattg cggccgtccg gctttcaaaa tggtgtaact
     1861 cttcacgata tctggcaatt aagagcaacg cgtagtcagt tgccgcacca ataacaagaa
     1921 cagacaaaat actttgcgat tgaccgtcta ctttgattat gtcgttactg gctaataagt
     1981 aaaccaatcc gccgcctact gagagtgcca agacagcaca aagtagtgga attatccaaa
     2041 ggactggcga ccgatataca aatatcaaaa tggcagcaac gatacccaaa gttttcaata
     2101 aaagaagtgt gtcaatgtca ccaaagctgc caaacagatc agccaagata ccgccaatac
     2161 cggttacatg agattcgaat ccatcaactg cattcgcttg agcacgaata gtttcaacaa
     2221 gaactggtaa ggccggttca ttattttcca gcggttgagc tatcactgaa tcatctatcg
     2281 gaattgaaat gatcgcagct ttattgtctt gcgatggaat cgggaaaagt ggagttccgt
     2341 cagctaggaa atcgccgatt gttttgccac cgctatctgg cacttctgca ttaggtaatg
     2401 tgccaacaaa ggttgtcagg tttgcaattg catttggatt tatttcacct gtgaaaataa
     2461 taattgctgg taatactgaa gagtcttcgg tgacaaactt tgtagaaatt tctccagctt
     2521 tggtcgactc tgctgaacca ggcaggaacc cggcactgtc attttcttgg acgctggtaa
     2581 gttttccaaa taatggacca gttgtaccgc ctataccaag ccaagcaacg attacgagga
     2641 ttgcaaacca caatgcttta cgtgcagttt tttcagtctt ttggcggcgg ccgtgtttag
     2701 ccatagttaa tggtccttag tggagttttg aaagtagata ccttcaaatt ctatttgacc
     2761 cgtgttttgg gtgcatcgtt gaccggtgtg cagaccgaac atttactcaa ttaggcttta
     2821 aatatgcctg tgcttatcga cagttcgaca ctgggcaagg tatttcataa ttacgaggaa
     2881 atgtggcaat accaacaaaa ggtccacgaa agtgttgtgg ccggcagttc tcccgacaca
     2941 attttgttac ttgagcatga accggtttat accgcagggc gaagaaccga aatttttgag
     3001 cgcccgacag acggtacgcc agtagtagac gttgatcgtg gcggaaaaat tacttggcat
     3061 ggccctggcc aacttgttgg ttacccgatt gtgcatttac ctgcggcgct tgatgtgatt
     3121 ccacacgtac gcagacttga agaggtgctc attaaaacgt gtgctgaatt tgaattaaca
     3181 acaacgcgaa taaaaggacg cagtggagtt tgggttaccg aaggaggctt ggatcgcaaa
     3241 gttgccgcga tcggcgttcg agttgcccaa ggtgtttcca tgcatgggtt tgcgttgaac
     3301 tgcaacaacg atttggattg gtttgatcat attgtcccct gcggtatttc agat
//
LOCUS       DBNHVN010000038         2054 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_378973, whole genome shotgun sequence.
ACCESSION   DBNHVN010000038 DBNHVN010000000
VERSION     DBNHVN010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_378973"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /gene="ald"
                     /locus_tag="AC16Y1_00655"
     CDS             complement(<1..914)
                     /gene="ald"
                     /locus_tag="AC16Y1_00655"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010987907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR5831007.1"
                     /translation="MKIGVPREIKNHEYRVAITPAGVHELVRHGHEVFIEHDAGVGSS
                     ITNEEYANAGAKILKTADEVWQTGEMIMKVKEPIKDEYHRMQKGQVLFTYLHLAAERA
                     CTDALLNMEVTGIAYETVELPDRSLPLLAPMSEVAGRLAPQVGAYSLMRANGGRGVLL
                     GGVPGVKPAKVVVLGGGVSGLHAAQIALGMGADVTILDLNVPRLRQIDTWYGGRIKTL
                     VSNSFSIESECIAADLVIGAVLVPGAKAPKLVSNKLVSQMKPGSVLVDIAIDQGGCFE
                     DSHATTHADPTFKVHNSVFYCVANMPGSV"
     gene            complement(961..1614)
                     /locus_tag="AC16Y1_00660"
     CDS             complement(961..1614)
                     /locus_tag="AC16Y1_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR5831008.1"
                     /translation="MISDQPVDHQVTNREEIFSGYVMDLRRDDFEFHGETLRREYIVH
                     PGAVAIMGMDHEGNLLLVQQYRHPQGKIMWEPPAGLLDAPDEDPLECAKRELLEETGY
                     EASKWEVLLDFANTPGGSSEQIRIYFAQGLKMHEGGRPPSSDEESSMTVHWVPLSEVL
                     ASIGQGLITNAALVVGSLALVTALAEPEIMLRPANSEWPARADVLKNGRVRLPKAKG"
     gene            complement(1623..>2054)
                     /locus_tag="AC16Y1_00665"
     CDS             complement(1623..>2054)
                     /locus_tag="AC16Y1_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase-related protein"
                     /protein_id="HIR5831009.1"
                     /translation="GLKDANSAEFDEKTTNPVISTMADQHDVVAGERDMGGTMRLGLY
                     PAKLKPGSIAARVYGADHAEERHRHRYEVNNAYREPLENAGLVFSGTSPDGHLVEYVE
                     LPVDVHPYYVATQAHPELKSRPTRANPLFTGLIEAALARQK"
BASE COUNT          539 a          501 c          433 g          581 t
ORIGIN      
        1 accgaacctg gcatgtttgc tacgcaatag aacactgagt tgtgaacttt gaaagtcgga
       61 tcagcatgtg tggtggcatg tgaatcttca aaacagccac cttgatcaat cgcgatatct
      121 acaagtactg aacctggttt catttgtgaa acaagcttgt tgctaactag ctttggcgct
      181 ttggcaccag gcacaagtac tgcgccgatt actaagtcag cagcaataca ttcagactca
      241 attgagaaac tatttgaaac taaagtcttg attcgaccgc cgtaccaagt gtcgatctga
      301 cgaaggcgcg gcacatttag atcaagaatt gttacatctg cgcccatacc aagtgcgatc
      361 tgtgctgcgt gtaatcctga gacaccgcca ccaagtacaa ctactttggc tggctttact
      421 ccaggtacgc cgccaagtag caccccacga ccaccgttag cgcgcattaa tgagtaggca
      481 ccaacctgag gtgcgagtcg acctgctact tcggacatcg gggcaagtaa cggaagtgag
      541 cgatctggta actcaacagt ttcgtaagca ataccagtta cttccatgtt aagtaacgca
      601 tcagtacaag cacgttcagc agccaagtgc aggtaagtga aaagtacttg gcctttttgc
      661 atccgatggt actcatcttt aatcggctct ttgactttca taatcatttc gccggtctgc
      721 caaacttcat cagcagtctt caaaatcttt gcaccagcgt ttgcatattc ttcgtttgtg
      781 atcgaagaac caacgccggc atcatgttcg ataaaaactt cgtgaccatg gcgtactaat
      841 tcgtgcacac cagctggcgt aatcgctacg cgatattcgt gattcttaat ttcgcgtgga
      901 acaccgattt tcattagtta cctctcttgg tgaccagtcc cgaactagcc ttcataaact
      961 ctatcccttg gctttaggta gtcgaactcg cccatttttc aatacatctg cgcgtgctgg
     1021 ccattcacta ttggctggac gaagcataat ttcaggttca gctaatgctg ttactaatgc
     1081 gagtgaacca actactaatg cagcgttggt aatcaaaccc tgtccgattg aagccaacac
     1141 ttcacttagc ggtacccagt gaacagtcat agaactttct tcatcactac ttggcggtcg
     1201 accaccttca tgcattttta aaccttgggc aaaataaatt cgaatttgct cgcttgatcc
     1261 accaggtgtg ttagcaaaat cgagtaacac ttcccactta cttgcttcgt aaccagtttc
     1321 ttcaagtagt tcacgttttg cgcattcaag tggatcttca tcaggtgcat caagtaatcc
     1381 agcaggtggt tcccacatga ttttgccttg tggatggcga tattgctgaa ctaaaagtaa
     1441 atttccttcg tgatccattc ccataatggc tactgcccca ggatgcacaa tgtattcgcg
     1501 gcgtaatgtt tcgccatgaa attcaaagtc atcgcggcgt aaatccatta catagccgct
     1561 gaatatttct tcgcggttgg ttacctgatg gtcaaccggc tgatccgaaa tcatctggct
     1621 gtttattttt gacgcgctaa ggcggcttcg attaatccag tgaaaagtgg atttgctcga
     1681 gtcggacgtg actttaattc aggatgtgct tgtgtagcga cgtaatacgg atgcacatca
     1741 actggtagtt caacatattc aactaagtgt ccatcaggtg aagtacctga gaaaactaaa
     1801 ccagcatttt ctaatggctc acgatatgca ttattaactt cgtagcgatg acggtgacgt
     1861 tcttcggcat gatctgcgcc ataaacccga gctgcaatac tgcctggctt taatttggct
     1921 ggatacaaac caagacgcat tgtgcctccc atgtcacgct caccggcaac tacatcatgt
     1981 tgatcagcca ttgttgaaat aacaggatta gttgtttttt catcaaactc agcagagttg
     2041 gcatctttta accc
//
LOCUS       DBNHVN010000039         4003 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_972783, whole genome shotgun sequence.
ACCESSION   DBNHVN010000039 DBNHVN010000000
VERSION     DBNHVN010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_972783"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="AC16Y1_00670"
     CDS             <1..1126
                     /locus_tag="AC16Y1_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831010.1"
                     /translation="IGPVGSSYTFTCLLKSVGNSGYSGMGFTALNAGSGNSSGSPYRP
                     NDALGLSVHGGGFVLHNGANSDIFGNWSGGAAGVQTIKNSAISDLLNNGSPDQWYKII
                     FKLTRDSATTFDARIEVWPTNSSGTELRANEADAIYEWLNVTNTNLINAPSIYSYINF
                     SGYRVTHFDNYRVDLAGGSTVVAAGTPVVLTNSATNSTGVVSFAGNVTSDGGSSVSER
                     GFAYGTSASPTISNNKVATGTGTGSFSGTTSALANGTYYFRAYATNATGTTYGSDIQV
                     VITDSAGAAPSSSPTPDPNAGSSGSNSNVSTTAAGTENVSNADSDQLARTGATDSPML
                     ALALILMFFGYTAYSIGFEEMRKAKVLKWLNLDWLDFKIK"
     gene            1139..1212
                     /locus_tag="AC16Y1_00675"
     tRNA            1139..1212
                     /locus_tag="AC16Y1_00675"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1173..1175,aa:Pro,seq:ggg)
     gene            1406..1618
                     /locus_tag="AC16Y1_00680"
     CDS             1406..1618
                     /locus_tag="AC16Y1_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831011.1"
                     /translation="MAAIENSDGTRKGINDAVFSGAGVVVPADKSLLGTEIAIDPATG
                     DIKTASVTIYVVQGGAEVDLMPWVVQ"
     gene            1847..3112
                     /locus_tag="AC16Y1_00685"
     CDS             1847..3112
                     /locus_tag="AC16Y1_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR5831012.1"
                     /translation="MIKPVKIGALVVAGALALAACGGGSDDAASGDGGKTLVISTDLP
                     LQGASADASASTNNVIKLYLEQIGGKVGDYTIELKEYDNSTAAKGSWDDAQCAKNAQD
                     HVANAAEVAVMGTYNSGCAKIIVPVLNQAPDGPMLMVSHANTNPGLTKTWDPGEPEKF
                     YPTGKRNYARVVTTDDNQGVAAAQFLKQERGVTKCAVLNDNQTYGQGVATAVKGELAN
                     QGIEVVVDEAWDAKQPNYTALMQKVKSAGADCLYFAGIYDNNGGQLVKDAVAVLGTPS
                     EFVMMAPDGFTGYPDLQGLPEAEGLLLSFTGLSQDGILAAGGKAAEFVAAYEAKYGSK
                     PVGSFSIYGGAAFQVILAAIEASDGTRAGINNAVFSGAGITISAADSVTGAEIKIDPA
                     SGDVVTKTITIQILKGGVETDVMPWAIAQ"
     gene            3313..>4003
                     /locus_tag="AC16Y1_00690"
     CDS             3313..>4003
                     /locus_tag="AC16Y1_00690"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5831013.1"
                     /translation="MTVTAAAKSRATISLSKILFRASLAFIIAGVLWWLGLNFVKDPA
                     QFATIAIFGLNNGALYALIALGYTLVYGIIELINFAHGDLFMLSAVFSTFMLVDILDV
                     TGQSTVNYIWVIAVLFMSMIFGALINVTIERLAYRKLRTAPKLAPLITAVGVSFILQF
                     VGLQWNGSAPRNFTTVIPDTTYVVAGVQIRIETILIYAVTIPLLIVLATLVNKTRQGR
                     AMRATAQDKDAS"
BASE COUNT         1106 a          882 c          945 g         1070 t
ORIGIN      
        1 catcgggcca gttggtagta gttacacatt cacctgtctt ctaaaaagtg ttggcaatag
       61 tggctacagc ggaatgggct ttacagcgct aaatgcaggt tcaggaaatt cttctggaag
      121 cccataccgt ccaaatgatg ctcttggact ctcagttcat ggcggcggat tcgttttgca
      181 caatggcgcc aattcagaca tttttggaaa ctggagcggt ggtgcagccg gagttcaaac
      241 tatcaaaaat tcagcaatat ccgatttact aaataatggc tctccagatc agtggtacaa
      301 aattattttt aaattaacta gagacagtgc aacaactttt gatgctcgaa tagaagtatg
      361 gccaacaaac tcatctggca cagaacttag agcaaatgaa gctgatgcga tttatgagtg
      421 gcttaacgta accaatacaa atttaattaa tgctccctcc atctatagtt atataaactt
      481 ttctggttat agagtcaccc attttgataa ttatcgagtc gacctagccg gtggttctac
      541 agttgttgca gctggcacac cggttgtatt aaccaatagt gcgacaaata gtactggggt
      601 agtttcattt gctggaaatg tgacctcaga cggtggaagt tcagtttcag agcgtggatt
      661 tgcgtatgga acgtcagctt caccaactat ttcaaataac aaagtagcta caggcactgg
      721 aacaggtagt ttttcgggaa cgacttctgc actagcaaat ggaacctatt actttcgggc
      781 atatgctacg aatgcaactg gaaccaccta cggttcagac attcaggttg tgatcacaga
      841 ttctgctggt gccgcaccat ctagtagccc aactccggat ccgaatgcag gctcatcggg
      901 atcaaattca aatgtttcta caactgccgc gggtactgaa aacgtttcga atgctgattc
      961 ggatcaattg gctcggaccg gtgccactga ttcaccgatg ttggcccttg ctttaatttt
     1021 gatgttcttt ggttacaccg catattcaat tggttttgaa gagatgcgca aagccaaagt
     1081 tcttaagtgg ctcaatcttg attggctaga tttcaagata aagtaaactc aaaattcacg
     1141 ggctgtagcg cagtttggta gcgcacttcg ctgggggtga aggggccgca ggttcaaatc
     1201 ctgtcagccc gactcaaatt tatttttggg ctgatcccca aaaataaatt tgaatttcgg
     1261 aagttgggcc aggatttggg ggccgatttc aggattgcat gcaatccgat aaatcggatt
     1321 taaatagcga gcgacgattc ctgtcagccc gacagatcca gttagctcat cctctatcta
     1381 tggtggagct gcgctacaag ttatcttggc ggcaatcgaa aattcagatg gtactcgtaa
     1441 ggggattaac gacgcagtat tcagtggtgc aggtgtagtt gtcccagcgg acaagtcact
     1501 actaggcact gaaattgcaa tcgatccagc aactggtgac ataaaaacag ccagcgtaac
     1561 tatttacgtg gtgcagggtg gcgctgaagt cgacctaatg ccatgggtag ttcagtaatt
     1621 ctgctggttt aactagtcag aacacttaag tcccgatatt gcctaaaact aggcgatatc
     1681 gggactttgt gcatttgtgc caaaaataac gtaatagtta cataggtaac gggttcataa
     1741 ctaagtgcca gatatggctt tattgagttt cctctgaggg gatatccacg taatttagga
     1801 catgcccgaa aagaattcat cgggcttcca tgagaggaaa caataaatga tcaagccggt
     1861 taagatcggt gccttggttg ttgctggtgc tctagctctt gctgcctgtg gcggcggaag
     1921 tgatgatgca gctagtggcg acggtggtaa gactctggtt atttcaacag atctaccatt
     1981 acagggcgca tctgctgatg cttcagcatc aacaaacaac gtaatcaagc tgtacctaga
     2041 acagattggt ggcaaagtag gcgactacac aatcgagttg aaggaatacg acaactcgac
     2101 agctgctaaa ggttcttggg acgatgcaca gtgtgcaaag aacgctcaag atcacgtagc
     2161 aaatgctgct gaagttgcag taatgggaac ctacaactca ggttgtgcaa agatcatcgt
     2221 gccagtgctt aaccaagcac cagacggccc gatgctaatg gtttcacacg caaacacaaa
     2281 ccctggtcta accaagactt gggatccagg cgagcctgaa aagttttacc caacaggcaa
     2341 gcgtaactat gcacgtgttg ttacaactga cgacaaccaa ggtgttgccg ctgcacagtt
     2401 cttaaagcaa gagcgtggag taacaaagtg tgcggtactt aacgacaacc agacttacgg
     2461 tcaaggtgta gcaaccgctg ttaagggcga acttgcaaac caaggcatcg aagttgtagt
     2521 tgacgaagca tgggatgcaa agcaacctaa ctacactgcg ctaatgcaga aagttaagtc
     2581 tgctggtgca gactgcttgt acttcgcagg tatctatgac aacaatggtg gacagttagt
     2641 taaggacgca gttgctgttc ttggtacacc atcagaattc gtcatgatgg ctccagatgg
     2701 cttcaccggt tacccagatc tacagggtct accagaagct gaaggtctgc tactttcatt
     2761 caccggtcta tcacaagacg gcatccttgc cgctggtggt aaggcagctg aattcgttgc
     2821 agcttacgaa gctaagtacg gttcaaagcc agttggttca ttctcaatct acggtggagc
     2881 tgccttccag gtaatccttg ccgcaattga ggcatctgac ggtactcgtg caggcatcaa
     2941 caatgctgta ttcagcggcg caggtatcac tatctctgct gctgactcag taacaggtgc
     3001 agaaatcaaa atcgacccag catcaggtga cgtagtgact aagaccatta ctatccagat
     3061 tcttaagggt ggagttgaaa ctgacgtaat gccttgggct atcgctcaat aattacgact
     3121 gcataaacag taaaactggg tggggttagg aaactaaccc cacccagtta tctaaataac
     3181 caaaatttaa ttcaaaatta aacaaaacgg atccagaaat gtatccaaat aaattttttg
     3241 ctaagcctac tttttttgtg ctctagccat tagatttatc taactacaac tcttaccggg
     3301 gagaagctac atatgacagt gacagctgcg gcgaagtcgc gggccacaat aagtctcagc
     3361 aaaattctct tcagagcatc tctagcgttc atcatcgctg gtgtgctctg gtggctgggc
     3421 ttgaactttg taaaagatcc agcacagttt gcgacgattg caatttttgg ccttaacaac
     3481 ggtgcactct atgcacttat cgcattgggt tacacattgg tgtacggcat tatcgagctg
     3541 atcaacttcg cccacggtga cctgttcatg ctctcagcgg tattcagcac attcatgttg
     3601 gtagacattc ttgatgtcac cggacaatcg acagtgaatt acatttgggt aatcgcagtt
     3661 ttgttcatgt caatgatttt cggtgcactg attaatgtga cgatcgagcg tctggcctat
     3721 cgcaaattgc gaacggcgcc aaaacttgca ccattgatta ccgctgtcgg tgtttctttc
     3781 attctgcaat tcgttggatt gcagtggaat ggttcagcgc cgcgtaactt cacaactgtt
     3841 attccagaca ccacctatgt agttgctggc gtacaaatcc gaattgaaac tattctgatt
     3901 tacgcagtca cgatcccact tttaatcgtt cttgctactt tggttaacaa gacccgtcaa
     3961 ggtcgggcca tgcgggcaac tgctcaagat aaagatgctt cac
//
LOCUS       DBNHVN010000040         1941 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_155021, whole genome shotgun sequence.
ACCESSION   DBNHVN010000040 DBNHVN010000000
VERSION     DBNHVN010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_155021"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /gene="gltX"
                     /locus_tag="AC16Y1_00695"
     CDS             <1..1108
                     /gene="gltX"
                     /locus_tag="AC16Y1_00695"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR5831014.1"
                     /translation="KTPGYDGVCRNLTDEQVTKFKTEGRAPVVRMRMPDTDWTWHDLV
                     RGDVTFAAEHVPDYVIVRATGEPLYTLVNPVDDVLMEVTHVLRGEDLLSSTPRQLALY
                     DALKAIGVAKGERPLYGHLPYVMGEGNKKLSKRDPESSLLMYREEGYLPEGLLNYLSL
                     LGWSFGEDREFFSKEEMAKVFEIERVNANPARFDLRKCTAINAEWIRTLEVSDLANRL
                     IPYLSSEGVFVDQPSAPDKELILQAIPLIQERMETLRQGANMLAFLFLGSAGSPEFEV
                     DPDEAAKVLNAESQPVLIEALAALNQVNDWTTEKIEQALRVALIDGLGLKPKVAFGPV
                     RVAVTGRRVSPPLFESLELLGKARSLSRIQAAII"
     gene            complement(1139..1211)
                     /locus_tag="AC16Y1_00700"
     tRNA            complement(1139..1211)
                     /locus_tag="AC16Y1_00700"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1175..1177),aa:Glu,seq:ctc)
     gene            complement(1265..1336)
                     /locus_tag="AC16Y1_00705"
     tRNA            complement(1265..1336)
                     /locus_tag="AC16Y1_00705"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1301..1303),aa:Gln,seq:ctg)
     gene            complement(1424..>1941)
                     /locus_tag="AC16Y1_00710"
     CDS             complement(1424..>1941)
                     /locus_tag="AC16Y1_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012879116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR5831015.1"
                     /translation="FAELAVAAGEDRLTAVANPVLLALRDATKESAQLYRRQGDHRIC
                     VAAAERMTGLRDTVPIGSTLTMQAGSAAQVLLAWEETDRLHLGLRGAKFSAADLSAVR
                     RNGWAQSIGQREAGVASVSAPVRSANGKVIAAVSISGPIERLSKTPGRLHGKAVVTAA
                     SRIGELLQSRG"
BASE COUNT          525 a          476 c          452 g          488 t
ORIGIN      
        1 taaaactcct ggctacgacg gcgtttgtcg caacttaact gatgagcaag ttacaaagtt
       61 caaaacagaa ggtagagcac cggtggttcg gatgcgcatg ccggacacgg actggacgtg
      121 gcatgacttg gttcgcgggg atgtaacgtt cgctgccgaa catgtgcctg attacgtgat
      181 tgtgcgcgca actggtgaac cgctttacac actagtaaat ccggtagatg acgtattaat
      241 ggaagtaacg catgtactgc gcggtgaaga tttacttagt agcacgccaa gacaacttgc
      301 tctttatgac gcacttaaag ccattggggt agccaagggc gaacgtccgc tttatggcca
      361 ccttccttat gtaatgggcg aaggaaataa gaaactttct aagcgagacc cagaatctag
      421 cttgttaatg tatcgcgaag aaggctacct acctgaaggt ttactcaatt acctatcctt
      481 attaggttgg tccttcggtg aagaccgtga attcttttcc aaagaagaaa tggccaaagt
      541 ttttgaaatc gagcgagtaa atgcaaatcc agcacgtttt gatttacgaa agtgcacggc
      601 aattaatgct gaatggatta ggactctcga ggtttccgat ctagcaaata gattaattcc
      661 gtatttaagt tcagagggtg tcttcgtcga tcaaccaagt gcgccagata aagaattaat
      721 ccttcaggca attccattaa ttcaggaacg aatggaaact cttcgccaag gcgcaaatat
      781 gcttgccttc ttattccttg gcagtgccgg atcgccagag tttgaagtag atccggatga
      841 agccgccaaa gtactaaatg ctgaaagcca acctgtttta attgaagcac tagccgctct
      901 gaaccaagtc aatgactgga ctactgaaaa aattgaacaa gctcttcgag ttgctctgat
      961 tgatggcctt ggcttgaaac ctaaagttgc atttggtcca gtgcgagttg cagtgactgg
     1021 tcgacgggtt tcgccgcctc tttttgagtc gctggaatta cttggcaaag ccagatcgct
     1081 atcgcgtatc caagccgcca taatttaaag ttgctcggca acaaagttaa ttattaagta
     1141 gccccgacgg gatttgaacc cgcgctaccg ccttgagagg gcggcgtgct aggccgctac
     1201 acaacggggc cctagcgaaa aagagtctga ctaatcgata gaactgttaa accctttttc
     1261 tcagctgggg taccaggact cgaacctaga ctagctgaac cagaatcagc tgggctgcca
     1321 attaccccat accccaatgg ctttactggc taactatgcc cagcaacctt tccgaattct
     1381 accgacttaa tttgaatatc taaaaagcca caaatcttaa agattaaccg cgactttgaa
     1441 gtaactcacc gattcggctg gctgcagtaa ctacagcttt gccgtgtaat cgccccggag
     1501 ttttagatag tcgctcaatg ggtccagaaa tagataccgc ggcaatcact tttccattcg
     1561 ccgaccgaac tggggcagat accgaagcga ctcccgcctc acgctgacca attgattgtg
     1621 cccaaccatt tcgccttact gctgataaat cagcagcaga aaactttgcg ccgcgcaatc
     1681 ctagatgtaa ccgatctgtt tcttcccagg ccagcaatac ttgagccgca gatccagcct
     1741 gcatggtcaa agtgctaccg atcggaaccg tgtcgcgaag gccagtcatt cgttcagccg
     1801 cagccacaca aattcggtga tctccttgcc gtcggtataa ctgcgcactt tcttttgtgg
     1861 catctcgtaa agccaataag actgggtttg ccactgcagt aagtcgatct tcaccggcag
     1921 caactgctag ttctgcaaaa c
//
LOCUS       DBNHVN010000041         2192 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_527670, whole genome shotgun sequence.
ACCESSION   DBNHVN010000041 DBNHVN010000000
VERSION     DBNHVN010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_527670"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="AC16Y1_00715"
     CDS             <1..709
                     /locus_tag="AC16Y1_00715"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5831016.1"
                     /translation="RVIAVEMNAETLAETQKLAGGDKVTTFVLDVTDQAAVAALPGKV
                     EGQVGEIDLLINNAGIIQPFVPIKDLTLEQAQKVMNVNFYGPLFMIKAFLPTLISRPE
                     AHLLNVSSMGAYAPVPGQSLYGASKAAVKLLTEGLRSELIDTNVGVTVAFPGAVSTNI
                     TTNSGLKLPANAEEMSAKFKSLDAAIAAKIMVNAIESGKPRVTVGKDATMMDRLSRLN
                     PVMAANIINKQMKSLLN"
     gene            complement(698..2041)
                     /locus_tag="AC16Y1_00720"
     CDS             complement(698..2041)
                     /locus_tag="AC16Y1_00720"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR5831017.1"
                     /translation="MKRSAIAAIAMFLSFAPAAPSSAQDSPLVVMSRNIYLGADVGIA
                     MELLPNFPAATQFMWDQMRQTDFESRSSGLVAEISQEVPDVIGIQEGTRWYCQAGPLS
                     DEVVVFDFVDILLEQLRSAGLDYVIATKDGHTAQNVGFKIAPIPLLTKATDPEIFPDL
                     FGNSSAYCGFEIADVLLVNGKLAQDVLAVGNTEYSQKYTIIPTVMSVYRGYSWADISI
                     DGKPVRFVTTHLESLFSEDDVPNSKIQADQLVSNLADTKIPIVLIGDFNSDPRDPRPR
                     SGPNPGLQPSENSLCAKQIDNPSIETADATCNAFWTISRGGFTSVGPDELASTNFTWG
                     INALLTGPDPLRSQMATEMGNTAGFTDRLDYIFIKGPIEVTGAKLVGHVSGQKWASDH
                     AGIVASLNLSEPIEYQEVRLAENMRFPLGFWQIFPVIIISAATFFIVKRKRKLIK"
BASE COUNT          629 a          497 c          471 g          595 t
ORIGIN      
        1 ccgggtaatt gcagttgaaa tgaatgccga aactctagct gaaacgcaaa agttagcggg
       61 tggcgataaa gtcactactt ttgttcttga cgttactgat caagctgcag tggccgcact
      121 gccgggaaaa gtagaaggcc aagttggcga aatcgactta ctgataaata acgccggaat
      181 aattcagcca ttcgtaccga ttaaagatct gacactggag caagcgcaaa aagtcatgaa
      241 cgtaaatttt tatggaccac tatttatgat caaggcattt ttaccaactt taattagccg
      301 acctgaagct cacttgctga atgtatcctc gatgggtgcc tacgctccag ttcctggcca
      361 gagtctttat ggtgcatcta aagctgcagt gaaattattg actgaaggtt tgcgctcgga
      421 gttaatcgat acaaatgttg gcgtaacagt ggccttccca ggagcagtga gtacaaacat
      481 cacaaccaat tctggtttga agttgccggc gaatgctgaa gagatgagtg caaaattcaa
      541 atctcttgat gctgcaattg ctgccaaaat tatggttaat gcaatcgaat ctggaaaacc
      601 tagagtaact gttggcaaag atgcgaccat gatggatcgt ttatctcggt taaatccagt
      661 tatggccgca aacattatta acaagcagat gaagtcgcta cttaattaac tttcgctttc
      721 ttttgacaat aaaaaatgtg gcagccgaga taatgatgac tggaaaaatt tgccagaatc
      781 cgagtggaaa tcgcatattt tcagctaacc gaacttcttg atactcaatt ggttcactta
      841 aattcaaact tgcgactatc cccgcatgat ctgaagccca cttttgcccg ctaacatgac
      901 caacaagttt tgctccggta acttcgatcg ggccctttat aaagatgtaa tcaaggcgat
      961 ctgtgaagcc agcagtattt cccatttcag ttgccatttg tgaccgaaga ggatccgggc
     1021 cagtgagtag ggcgtttatc ccccaagtga aattagtcga agctaactca tctggaccga
     1081 ctgaagtaaa gccacctcta gaaatggtcc agaacgcatt acatgttgca tctgctgttt
     1141 cgatagaagg attatctatc tgcttggcgc acaggctgtt ttcactcggc tgtaaacctg
     1201 gatttggtcc actcctgggt cgtgggtcac gtggatcact attgaagtcg ccaattaaaa
     1261 caatcggaat tttcgtatca gccaaattgg aaacaagttg gtcggcctga attttagaat
     1321 ttggcacatc atcttcggaa aacaaggact ctagatgagt agtaacgaaa cgaactggtt
     1381 taccatcgat cgaaatatct gcccaactat atccgcggta aaccgacatc accgttggaa
     1441 taatggtgta cttctgactg tattcagtat ttccaactgc tagtacatct tgggctagct
     1501 tgccatttac caacaagaca tcagcgatct caaacccgca atacgccgag ctgttaccaa
     1561 ataggtcagg gaagatttct ggatcggtgg ccttggtcaa aagcggaatt ggcgcaatct
     1621 taaagccaac attttgcgcg gtatgtccat ctttagtcgc aataacatag tccaaccctg
     1681 cgcttcgcaa ttgctctagc aggatgtcca caaaatcaaa taccaccact tcatccgaaa
     1741 gcggtccggc ttggcaatac cagcgtgtac cctcttgaat tccaattaca tcgggtactt
     1801 cttgagaaat ttctgctacc agcccactac tacgactttc aaaatcagtc tggcgcattt
     1861 gatcccacat aaattgcgtc gccgctggaa agtttggaag taactccatc gcgataccaa
     1921 catcagcgcc taggtagatg tttctagaca tcacaactaa cggagaatct tgtgcagatg
     1981 aaggtgcggc cggcgcaaat gagagaaaca ttgcaatcgc cgcaatagct gaccgcttca
     2041 ttctttaacg attactttct aactcatcca gttttgaaat cactgtttga attgaaggat
     2101 tcgtcattgc ctttccgtca gcaaaaacta aagtcggaac ggtgcgattt ccttcattaa
     2161 cctgttcaac tatttgagca gcttctggat tt
//
LOCUS       DBNHVN010000042         4527 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1791310, whole genome shotgun sequence.
ACCESSION   DBNHVN010000042 DBNHVN010000000
VERSION     DBNHVN010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1791310"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="AC16Y1_00725"
     CDS             complement(<1..1093)
                     /locus_tag="AC16Y1_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D-related
                     protein"
                     /protein_id="HIR5831018.1"
                     /translation="MTTQIRHGLTNLPFHSPVTHGFTEFDLEVQDGKVLNAKPIFGSM
                     HRGAEKLFESRDYRQILSLANRHEWLGSFAGELGIAQLIEDALGIEAPIAAQWLRTYL
                     LEYTRVTSHLAFIAGFPWVDKAISQTVRENRELWINHIAQFTGNRMHVMVSRIGGISH
                     APTASWLNQTNELVSKTQTLLNQLDTQIQNELEKYKDVGKLTYADAIEFAVSGPVARA
                     ASYQIDLRQSARGLKYSELKPTKHSINSDDLPGRINQLLAEVTQALVWLTELEPIAAQ
                     QLTEQLDVLLPKVLRVPEGVYHHDLETPLGIASWLLISHNDKMPYRLKLRPASLSTLL
                     ATEKVLVGVSLENVDAVIASMPFISGDVDR"
     gene            1137..2051
                     /locus_tag="AC16Y1_00730"
     CDS             1137..2051
                     /locus_tag="AC16Y1_00730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003992008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HIR5831019.1"
                     /translation="MVNTVAESDMPWTEKPPRLDIGVIGTGRVGAVLGAALNRAGHKV
                     VAASAVSDISRLRAESLIPTTKIMPINEVVKDRDLILLTVPDDVLPELVSGLAKTKSV
                     SPGSFVVHTSGRLGTKVLAPLTEQGCLPLAIHPVMTFTGTSIDLTRLSGCPFGVTAPE
                     SLRPVAEALVVEIGGEPIWVPESNRNLYHAALAFGANNLITIVNETVELLSKAGIENP
                     TDIVSPLLHASLDNALRNGDASLTGPISRGDVTTVKAHINELTKISPATVAAYKAMGR
                     LTLDRALEAGLINPQQAQALLDVLSSNQ"
     gene            2048..2914
                     /gene="panC"
                     /locus_tag="AC16Y1_00735"
     CDS             2048..2914
                     /gene="panC"
                     /locus_tag="AC16Y1_00735"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIR5831020.1"
                     /translation="MTQVINTISDFRTWQSKQDFPTVAVFTMGALHDGHQKLIKYARQ
                     YINEKLSGNGKVVVSIFVNPTQFNNSADLEKYPRDLDADLKICKELQVDVVFAPTAQE
                     MYPTGETLIKISQPPMASDLEGQSRPGHFVGMLTVVNKLLNITNPVATFFGEKDFQQL
                     VLVQQMVADLNLPIQVIGVPTVRDEDGLALSSRNKRLTAKERQVASQIPATLSLIKHS
                     LTEGHTLTKAKELGLEYLTKFPELRIDYLELRNEQLGIPTENESVRALIAVQIGELRL
                     IDNMTVKDQSCC"
     gene            2905..3663
                     /locus_tag="AC16Y1_00740"
     CDS             2905..3663
                     /locus_tag="AC16Y1_00740"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018207134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR5831021.1"
                     /translation="MLLAIDVGNTNTVLGLFDGPDIYQSWRTKTDARETADELALKFR
                     ALLEDSPEVTGIALCSTVPAVLREMRTMLDRYYRDIHTIIVEPGIKTGVPVVTDNPKE
                     VGADRIVNSLAAFTLYGGPAIVVDFGTSTNLDVVSGKGEFLGGALAPGIEISIDALAA
                     RAAQLRKVELVKPRNVIGKNTVEALQSGAIYGFAGQVDGLVDRITEELGQEVKAVIAT
                     GGLAGIVVPESATITHHEPDLTLIGLRLMFERNA"
     gene            3737..>4527
                     /locus_tag="AC16Y1_00745"
     CDS             3737..>4527
                     /locus_tag="AC16Y1_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006371938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR5831022.1"
                     /translation="MQVRHEKRNQLLANGTDAYPVTVPVTTTIPAVRAKYPDLAAEAT
                     TGDVVGVAGRVMFLRNTGKLCFAQLQAGNGETLQAMLSFDKVGEANLENWKALVDIGD
                     IVAVEGEVISSKRGELSVLTDKWHMAAKALRPLPNPHKPLNEETRVRQRYADLIMRDT
                     AREVARLRPATLASLRASFAKRDFVEVETPMLQTMHGGAAARPFVTQSNAFDMELFLR
                     IAPELFLKRCVVGGIERVYEINRNFRNEGADSTHSPEFAMLEFYQA"
BASE COUNT         1305 a          994 c         1008 g         1220 t
ORIGIN      
        1 aacgatctac atcgccactg ataaacggca tacttgcaat tactgcatca acattttcta
       61 aactaactcc aactaaaact ttttcggttg ccagcaaagt agataatgat gccggcctca
      121 gttttagccg gtaaggcatt ttgtcgttat gcgaaataag taaccacgaa gcaattccaa
      181 gtggagtttc taaatcatgg tggtagacgc cttcgggcac tcgcaaaact tttggtaaca
      241 acacatctag ttgttccgtt aattgctgcg cggcaattgg ctcaagttca gttaaccaaa
      301 ctaaagcctg agtaacttca gcaagtagtt gattaattcg cccaggcaga tcgtcactat
      361 taatactgtg tttagttggc ttcaactcac tgtacttaag tccgcgcgca gattgccgta
      421 aatcaatttg gtagctagcg gctctggcaa ctggaccgct tactgcgaat tcaattgcat
      481 cagcataagt taacttgcct acatctttgt atttttctaa ttcattttga atttgcgtat
      541 ccaattgatt aagcaaagtt tgtgtcttac taactaactc atttgtttgg tttaaccaac
      601 tagccgtagg cgcatgtgaa attccaccga ttcgactaac cattacatgc attcggttac
      661 cagtgaattg cgcgatgtga ttaatccaaa gttcacgatt ttcccgcacg gtttgcgata
      721 tagctttatc aacccatggg aagcccgcga taaaagcaag gtgtgaagta acgcgagtgt
      781 attcaagcaa ataggtacgc agccattgcg cagcgatggg cgcctcaata cctaacgcat
      841 cttcaattaa ttgcgcaata ccaagttcgc cagcgaatga gccaagccat tcatggcgat
      901 tagccagtga aagtatttgt cggtaatcac gagattcaaa taacttctca gctccgcggt
      961 gcattgaacc aaaaattggt tttgcattaa gcactttgcc gtcttgaacc tcaagatcaa
     1021 attcagtgaa accatgtgta accggactat gaaaaggcaa atttgttaag ccatgtctaa
     1081 tttgcgtcgt cacctgactt atcgtgccac acctaaaaag ggtctaggct tattacgtgg
     1141 tcaacacagt ggccgagagc gatatgcctt ggaccgaaaa accgcctcgc cttgatatcg
     1201 gcgttattgg caccggccga gtaggtgccg ttcttggtgc tgccttaaat cgagccgggc
     1261 acaaagtagt tgcagcgagt gcagtttcag acatcagtcg attgcgcgct gaatcattaa
     1321 ttcccactac aaaaataatg ccgattaatg aggtagttaa agaccgcgac ttaattttgc
     1381 taactgtgcc agatgatgtg ctaccagagt tagttagtgg tttagcaaaa acaaaatcag
     1441 taagtcctgg ctcttttgtt gtacacacgt caggcagact tggcaccaaa gtccttgcgc
     1501 cattgaccga acaaggatgt ttaccactgg ctattcatcc cgtgatgaca tttaccggaa
     1561 caagtatcga cttaaccaga ctttcgggat gcccattcgg agtaactgcg ccagaaagtt
     1621 tgcgaccagt ggctgaggcg ctagttgttg aaattggtgg cgaacctatt tgggttccag
     1681 aaagtaatcg aaatttatat catgcagcgc ttgcattcgg tgcaaacaac ttaatcacca
     1741 tcgttaatga aactgttgag ttattaagta aagccggtat agaaaatcca acggacatag
     1801 tttcgcccct tttacatgct tccttagata atgcactacg taacggtgat gcgtcgctga
     1861 ctggccctat ttcaagaggc gatgtgacaa ccgtaaaagc gcacattaat gagttaacca
     1921 aaattagtcc agccacagtt gccgcatata aggctatggg ccgattaact cttgatcgcg
     1981 cactcgaagc aggattaatt aatccgcaac aagcgcaagc acttcttgat gtattgagta
     2041 gtaatcaatg actcaagtca taaatacgat ttcagatttt cgaacttggc aaagtaagca
     2101 agactttcca actgttgccg ttttcacaat gggtgcgctg catgatggtc atcaaaaatt
     2161 aattaagtat gcccgtcagt acatcaatga aaaattatct ggcaacggga aagttgttgt
     2221 ttcaattttt gttaatccaa ctcaatttaa taattctgct gatctagaaa aatatccgcg
     2281 tgatcttgat gctgacctaa agatctgcaa agaattacaa gttgatgtgg tttttgcgcc
     2341 aactgcacaa gaaatgtatc ccacaggcga aactctcata aaaataagtc agccaccgat
     2401 ggctagtgat cttgaaggtc aaagtcgtcc cggacacttt gttggcatgt taactgtggt
     2461 taataaattg ctgaacatca caaacccagt agccactttc tttggtgaaa aagattttca
     2521 gcaattggtt ttagttcaac agatggtggc agatctaaat ctgccaatcc aagtaattgg
     2581 cgtacccacg gttcgtgatg aagatggatt ggcgctatca agtcgcaata aaagattaac
     2641 tgccaaagag cgtcaagtag ctagccaaat tccggcaaca ctttccctaa ttaaacacag
     2701 tcttactgaa ggtcacactt taactaaagc taaagaactt ggcttggaat acttaactaa
     2761 atttccagag ctcagaatcg attatttgga actgcgcaat gaacagctcg gaatacctac
     2821 agagaatgaa tcagttcgcg cattaattgc cgtgcaaatc ggcgaactta gattaatcga
     2881 caatatgaca gtgaaggacc aatcatgctg ctagcaatcg atgtcggaaa taccaatacg
     2941 gtacttggat tatttgatgg tccagacatt tatcagtcat ggcgaacaaa aactgatgca
     3001 cgtgaaacag cagatgaact tgcccttaag tttcgtgcct tacttgagga cagtccagaa
     3061 gtaactggaa ttgccctttg ttcaactgtg ccagcggtat tacgtgagat gcgcacgatg
     3121 ttagatcgtt attaccgcga catccacacc atcattgttg aacctggcat taaaacaggt
     3181 gttccggttg ttaccgacaa tccaaaagaa gttggtgctg atcgaattgt taattcactt
     3241 gcagccttca cgctttatgg tggaccggca attgtggttg actttggaac cagcacaaat
     3301 cttgatgtgg tttcgggtaa aggtgaattt cttggtggcg cgcttgcacc aggtattgaa
     3361 atatcaattg atgctctagc agcacgtgct gcgcaactgc gcaaagtcga attagtaaaa
     3421 ccaagaaatg tgattggcaa gaacacagtt gaagcactgc aatcaggtgc aatctatggc
     3481 tttgctggcc aagttgatgg tttagtagat cgcattaccg aagaacttgg tcaagaagtt
     3541 aaagcggtaa ttgctactgg tggtttagct ggcatcgtgg ttccggaatc tgcaacaatc
     3601 acacatcacg aacccgacct gactttgatt gggttgcgcc tcatgttcga acgtaacgcc
     3661 taataacggt aaccttatct gcgtgactaa cgaagaaaac ttgcctgtcg atgacgccga
     3721 tgatctgcct gaacagatgc aagtgcgcca tgaaaagcgc aaccagttac tcgcaaatgg
     3781 caccgatgct tacccagtaa ctgttccggt cacgacaact attccggcag tccgcgccaa
     3841 gtatccagat cttgctgctg aagcaacaac aggcgatgtg gtcggagttg ctggtcgcgt
     3901 aatgtttttg cgcaataccg gaaaattatg ttttgctcag ctgcaagccg gaaatggcga
     3961 aaccttacaa gccatgctgt ccttcgacaa agtaggcgaa gcgaatcttg aaaattggaa
     4021 agcgttagtc gacatcggcg acattgttgc tgtcgaaggt gaagtgatta gcagtaagcg
     4081 cggtgaactt tcagttctta ccgacaaatg gcacatggcg gcaaaagcat tacggccgtt
     4141 accaaatccg cataaaccgc tgaacgaaga aactcgagtt cgccaacggt atgcagattt
     4201 aattatgcga gacactgccc gcgaagttgc cagattacgc ccagcaactt tagctagttt
     4261 gcgcgcaagt tttgctaaac gtgatttcgt tgaagttgaa actccgatgt tgcaaactat
     4321 gcacggcggc gccgcggcta gaccgtttgt aacgcagtca aatgcattcg atatggaatt
     4381 attcctacgg atcgcacctg agcttttctt aaagcgttgt gtagttggtg gtatcgagcg
     4441 ggtttatgaa atcaatcgca acttccgaaa tgaaggtgct gactcaacac attcaccaga
     4501 atttgccatg ctcgagtttt atcaggc
//
LOCUS       DBNHVN010000043         1206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1865864, whole genome shotgun sequence.
ACCESSION   DBNHVN010000043 DBNHVN010000000
VERSION     DBNHVN010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1865864"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="AC16Y1_00750"
     CDS             complement(<1..337)
                     /locus_tag="AC16Y1_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR5831023.1"
                     /translation="MNEMFKAPKGTYDVIPPFSNLWLQVKDALATPPKLSGYGYIETP
                     LFEDTELFVRGVGKSTDVVSKEMYTFEDKGGRSLTLRPEGTAGVLRAMLENRMDRGAL
                     PAKVWYTGPN"
     gene            complement(334..1023)
                     /locus_tag="AC16Y1_00755"
     CDS             complement(334..1023)
                     /locus_tag="AC16Y1_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5831024.1"
                     /translation="MIIDSFIAGPWQTNCYVLSPASGTECVVIDPGQNAVGPLREVIA
                     KHRLKPVAVLLTHGHLDHMWSVFPVASGYEIPALIHQADRHLLSDPGAGISAETRAIL
                     PQLVDADYVFTEPSEVIEVTDELSINLAGLDFQIRSTPGHTQGSVIYAVNSSEPVVFT
                     GDTLFAGAIGRIDLPGGSASAMTATLRDIICKLPEDALIYPGHGPKSRMEIELQSNQY
                     LLRVQQGMSAV"
BASE COUNT          337 a          276 c          249 g          344 t
ORIGIN      
        1 aattaggccc ggtgtaccaa actttcgctg gtaacgctcc tctgtccatt cggttctcga
       61 gcatcgctcg caaaactccc gcagtaccct ctggtcttaa ggtaaggctt cgaccaccct
      121 tatcttcaaa tgtgtacatt tcttttgaca ctacgtcagt tgattttccg acaccacgga
      181 cgaataactc agtgtcttca aaaagtggcg tctctatgta cccgtatcca gaaagtttgg
      241 gtggtgtggc taatgcatct ttcacttgca gccacaaatt tgaaaacggt ggaatgacat
      301 cataagtacc ttttggtgct ttgaacattt cgttcatacg gcagacattc cctgttgaac
      361 tcgaagtaaa tactggttag attgcaattc aatttccatc ctggactttg gtccatggcc
      421 tggataaatc aatgcatctt ccggtaattt acaaataata tcgcgaagtg tggcagtcat
      481 tgctgatgcc gaaccacccg gtaagtcgat tcgtccaata gcacctgcaa acagtgtgtc
      541 accagtaaaa acaaccggtt ctgaactatt taccgcgtag atgactgaac cttgggtatg
      601 gcctggtgta ctccgaatct gaaaatcaag gccagccaaa ttaatactta gttcatctgt
      661 tacttcgata acttcacttg gttcagtaaa cacataatct gcgtcaacta actggggcaa
      721 aattgctctt gtttccgcag aaattcctgc tccaggatcg gaaagtaaat gtcgatcagc
      781 ctgatggatt agcgctggaa tctcatatcc gctagcaacc ggaaataccg accacatgtg
      841 atctaaatga ccgtgggtaa gtaagacggc taccggtttc aatctatgtt tcgcgattac
      901 ttcacggagc ggacccacgg cattttgacc tggatcgata actacgcatt cagtgccact
      961 ggctggtgat aatacatagc aattcgtttg ccaaggtcca gcaataaaac tgtcaattat
     1021 caatgattta ctcctaagta gtaggttacc gttttattcg attttttttg attgcataga
     1081 atgttatttg tgtctgttaa acgtgaaaaa gaactggccc gagctaaatt cgagcgccaa
     1141 caagccaaac gtcaacatcg taaaagaaga aacgtgttcg ttaaaataac cagcattgga
     1201 attgtg
//
LOCUS       DBNHVN010000044         8577 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1717516, whole genome shotgun sequence.
ACCESSION   DBNHVN010000044 DBNHVN010000000
VERSION     DBNHVN010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 8577)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8577)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8577
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1717516"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..1243
                     /gene="tgt"
                     /locus_tag="AC16Y1_00760"
     CDS             14..1243
                     /gene="tgt"
                     /locus_tag="AC16Y1_00760"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR5831025.1"
                     /translation="MAKSQFGFEVVKQLEPAQAELKPVGRAGVISTPHGQIQTPAFIP
                     VGTKATIKSVLPEAMKDLGAQALLANAYHLYLQPGPEIVDQAGGVGKFMNWPGPTFTD
                     SGGFQVLSLGVGFKKVLAMESVTMRSDDVIAEAKERLAHVDDDGVTFKSHLDGSMHRF
                     TPEFSMQVQHELGADIMFAFDECTTLLNTREYQEMSLERTRLWALRCISAHQDLTSQR
                     SHRPYQALFGVIQGAQYEDLRRKAAQDLAAMDFDGFGIGGALDKAQLGTIVRWVSEEL
                     PAQRPRHLLGIGEPEDLFVGVENGADTFDCVSASRVARNAAVYFPTGRFNISNAKYRK
                     SLDPLVPGCGCYTCTHYSQAYLNHLVHAKEMTAGTLLTIHNEYFIVNLVARIRESILS
                     GTFYDFKAEFLGQYQRN"
     gene            complement(1240..1935)
                     /locus_tag="AC16Y1_00765"
     CDS             complement(1240..1935)
                     /locus_tag="AC16Y1_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003936895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160254 - tRNA queuosine(34) biosynthetic
                     process from salvaged queuosine or its precursors
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HIR5831026.1"
                     /translation="MTRSENLTQTSAANYAATSKSYYPILSAVFVGLLIISNIGAVKL
                     ISAGPLIVDGGVFLFPLVYVVGDVLTEVYGFKAARRVIFVSFGMAVLAAFTFWLVQIS
                     PAGPGWENQAAYESILGFVPRIVLASVSGFLVGQLLNSWVLAKIKQRTKEKKLWVRLI
                     GSTAVGELADTVVFCTIAFYGVITGTDFINYVVVGYIYKTLLEVVLLPITYRVVRHVK
                     DREVTYSSHQELV"
     gene            1970..3217
                     /locus_tag="AC16Y1_00770"
     CDS             1970..3217
                     /locus_tag="AC16Y1_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5831027.1"
                     /translation="MSETVSTSLQTRTIRTLALAQILSGVGVAGTVAAGSLLVTSMTN
                     SETLAGIAQTFSVFGAAVMALVLANLTRRGGRRLALSAGYFIGAIGAAIAVIAGNSAL
                     VPLVFLGTFMIGASSAAGYQARYAAIDLADDKNRASSLSFVVWASTIGSVLGPNLLEP
                     LGQVASQFNLPELTGPFLMAALSLFAGSSLIWLRLRPDPYLTSLAGTKEVGAATPVKI
                     PFSETFGHIRSNSKSLLGISAIAIGHVAMVSIMVMTPVHMKHVDVSLRVIGLVISVHI
                     AGMYAFSPLVGKLSDKIGRVSVIAIAGLVLLSSAVIAGLASASNSTQLGIGLFLLGLG
                     WSGTLIAGSTLLSETVSDHYRASAQGVSDLIMNLSGAFGGAIAGVIIATLSYGWLCAL
                     AAIPVGVMTLRAIIARKNSLITG"
     gene            complement(3218..4213)
                     /locus_tag="AC16Y1_00775"
     CDS             complement(3218..4213)
                     /locus_tag="AC16Y1_00775"
                     /EC_number="3.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006935184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HIR5831028.1"
                     /translation="MTYRMPAEWEVHERTWMAWPVATYSAGTDSDIEIAYEAWANVAN
                     AIIEFEPVTMLVDPQNISAAKKWLDNRIELLPANLDDAWMRDIGPTFVRDEAGNIHGV
                     DWVFNGWGGQHWAKWDHDSQIANYILQETQIPCISSKLTNEGGGIHVNGAGTVLVTKT
                     VQLDPGRNPGWTASDVEAEFARTLSANNVVWLERGLTRDYDDYGTKGHVDIVATFCNP
                     DTILFHQQLNPAHPDFEVSKEVEATLSNTKFKLIPVPAPEVIRDDEGFVDYSYINHYV
                     CNGGVILCAFADPVDQHAKKIMEQAYPGRKVVLVDAREIFARGGGIHCITQQQPK"
     gene            complement(4227..5123)
                     /locus_tag="AC16Y1_00780"
     CDS             complement(4227..5123)
                     /locus_tag="AC16Y1_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019851937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5831029.1"
                     /translation="MSKTQDRIYPLLFVFLWSTGFVAARYIKPYAEPFTFLTYRYTIA
                     AVILLIAALIAKEKLRLTKSQFWSSIAVAMMLHVIYIGGIFLAVSLGVSSGVSAVIVS
                     LQPVLVGILAVPILGERLRRIQIVGLGLGIIGVAILLLPRIFQGDVSTNFTTAGIVTC
                     FVALLGTTGGYLAQKKTGGNIPFLTGTTVQYFASAIVFLILALVLENTSVTWNPEFIF
                     GLSWAVIANSIASIAILYALLRKGSAQKASSLYYLVPPSAAIMAFALFDERIEPSGLF
                     GMALAGLGVMLVMREVPVEETS"
     gene            complement(5152..6480)
                     /gene="argG"
                     /locus_tag="AC16Y1_00785"
     CDS             complement(5152..6480)
                     /gene="argG"
                     /locus_tag="AC16Y1_00785"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR5831030.1"
                     /translation="MSKVLASLPIGERVGIAFSGGLDTSVAVAWMRDKGAIPCTYTAD
                     LGQYDEPDIDSVPARAISYGAEISRLVDCKEPLVAEGLAAIACGAFHIRSGGRQYFNT
                     TPLGRAVTGTLLVRAMLEDNVEIWGDGSTYKGNDIERFYRYGLLANPNLRIYKPWLDQ
                     DFVRELGGRKEMSEWLVAHSFPYRDSVEKAYSTDANILGATHEAKTLEFLNESMEIVQ
                     PIMGVRFWDESVKISAEDVTIAFNQGFPVAINGKTFANNVALIKEANEIGGRHGLGMA
                     DQIENRIIEAKSRGIYEAPGMALLFIAYERLVSAIHNEDTIANYHAEGRRLGRLLYEG
                     RWFDPQSLMLRESLQKWVASAVTGEVTLRLRRGDDYTILDTKGPALSYDPEKLSMERT
                     ENAAFGPADRIGQLTMRNLDIADTRAKLELYRSQGQLDAKNFGIVGEIEN"
     gene            complement(6509..6853)
                     /locus_tag="AC16Y1_00790"
     CDS             complement(6509..6853)
                     /locus_tag="AC16Y1_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HIR5831031.1"
                     /translation="MSAKDIDQVLAKLDPAQRQTLEVVRQRILEIIPEAEQCIKYGIP
                     TFTINGKGVAGIAGNKNSCSFYPYSGSVLSQFPELADWSQTKSALHFPNDKPLSKTLI
                     RKLIKARLKLGI"
     gene            6908..7291
                     /locus_tag="AC16Y1_00795"
     CDS             6908..7291
                     /locus_tag="AC16Y1_00795"
                     /inference="COORDINATES: protein motif:HMM:NF019359.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TipAS antibiotic-recognition domain-containing
                     protein"
                     /protein_id="HIR5831032.1"
                     /translation="MNKNPYEEEARERWGDTDAYRDSATRLKSYSAEDLEMAKKEMRE
                     ATLIILDAMLAGLPADSPEAMAGAAAHRKSISDWWYDCSYEMHVNLAQMYVADERFSE
                     FYEQIHSGLSQYVYDAIFANALANS"
     gene            complement(7288..7794)
                     /locus_tag="AC16Y1_00800"
     CDS             complement(7288..7794)
                     /locus_tag="AC16Y1_00800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5831033.1"
                     /translation="MADLTAIHVVPELLSVKDWQRFREIRIASLKTDPEAFGGNLEQS
                     MQQTQSEWESQFGQITPVVATLRNKDVGLMTVENLAGDFGATCWIGGCWVKPEFRGHG
                     IMRKMFDYVDLNAQSKSWQVQGLGVWTTNHSAIAAYQALGFVSVGKPVPSKSKPGMFY
                     QRMIRKTS"
     gene            7849..8331
                     /locus_tag="AC16Y1_00805"
     CDS             7849..8331
                     /locus_tag="AC16Y1_00805"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HIR5831034.1"
                     /translation="MQLNEIDLTMLDGKKTTFGNYSDKVVLIVNVASRCGLAPQYETL
                     EAMQKKYGPRGFTVLGLPSNQFLQELNSSEAISNYCSTTWGVTFPVTEKVKVNGRNQH
                     PLYRELTKSKDASGKSGRIMWNFEKFLVLPNGEVHRFRPTTKPDDPAIIELIEKNLPA
                     "
     gene            complement(8297..>8577)
                     /locus_tag="AC16Y1_00810"
     CDS             complement(8297..>8577)
                     /locus_tag="AC16Y1_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007316718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR5831035.1"
                     /translation="RERGWSPVSKQHYLNEVNSGSLYVGSPETVSRKIARALSSVGAQ
                     RFDFKYVNGPLPQSQIMKSLELYGTQVIPRVRELLAEVKPANSFQLVQ"
BASE COUNT         2421 a         1985 c         1809 g         2362 t
ORIGIN      
        1 aaaatagtta atcttggcca aatcgcaatt tggctttgaa gttgttaagc aacttgagcc
       61 agcacaagct gaattaaagc cagtaggtcg tgcgggcgta atctcgaccc cacacggcca
      121 aatacaaaca ccagcattta ttccagttgg caccaaggca acaattaaat cagtactgcc
      181 tgaagcaatg aaggacttgg gggcgcaggc actacttgcc aacgcttacc acctttactt
      241 acaacctggg cctgagattg ttgatcaagc cggtggagtt ggcaagttta tgaattggcc
      301 tgggccaaca tttaccgata gcggtggctt ccaagtttta agccttggag ttggatttaa
      361 aaaagtgctg gcaatggagt cagtaaccat gcgctcagat gacgtaattg ccgaagccaa
      421 agaacgtctg gcccacgtcg atgacgatgg cgtcactttt aagtcgcatc tagatggctc
      481 aatgcacaga tttacgcctg agttttctat gcaggttcaa catgaactag gcgcggacat
      541 tatgtttgcc tttgatgagt gcacgacttt actcaatact cgcgagtacc aagagatgtc
      601 tctagagcga accaggcttt gggcattacg gtgtattagc gcgcaccaag atttaaccag
      661 tcagcgaagt catcgtcctt accaagcact ttttggcgta attcaaggag cccaatacga
      721 ggacttaaga agaaaagctg cccaagactt agcagcaatg gatttcgatg gttttggaat
      781 tggcggtgca ttagataagg ctcaacttgg cactatcgtg cgttgggtaa gcgaagagct
      841 cccggcgcaa cggcctcgac accttttagg tatcggtgaa cctgaggatt tattcgtcgg
      901 agttgagaat ggtgccgaca cttttgattg tgtatctgca tccagagtag ccagaaatgc
      961 tgctgtttac ttccctactg gtcgatttaa catctcaaat gctaagtaca gaaaatcgct
     1021 agatccacta gttccaggtt gtggctgcta tacctgcaca cattactcgc aggcttattt
     1081 gaatcatcta gttcacgcaa aagaaatgac cgccggaact ttacttacta ttcataacga
     1141 atacttcatt gttaatttag ttgccagaat tcgagaaagt atcctgtcgg gaactttcta
     1201 cgatttcaaa gctgagtttc ttgggcagta tcagcgaaat taaaccaatt cttggtgtga
     1261 actgtaagta acttctcgat ctttaacgtg cctaacgaca cgataagtaa tcggaagtaa
     1321 aacaacttcc agcaatgtct tgtaaatgta gccaacaact acatagttaa taaaatcagt
     1381 gccggtgata actccataaa atgcgatcgt acaaaaaaca actgtgtcgg ctaattcgcc
     1441 aactgccgtg gagccaatca acctgaccca aagttttttt tctttggtgc gctgcttgat
     1501 tttggctagc acccaagaat ttaagagttg accaaccaga aaaccagaaa cactggccag
     1561 cacaattctt ggcacaaacc caaggatgga ctcatatgca gcctggtttt cccaaccggg
     1621 gcctgctgga gaaatttgaa ctaaccagaa agtaaacgca gcaaggactg ccatcccaaa
     1681 acttacaaaa ataacccgac gagcggcttt aaagccatag acctcggtta aaacgtcacc
     1741 gacgacataa accagcggga aaagaaatac tccaccatcg acaattagtg ggccagctga
     1801 gattaattta actgcgccta tgtttgaaat aattaagagt ccaacaaata cagcgctaag
     1861 aattgggtag taactcttgg aagtagccgc gtagttggct gctgacgtct gggttaaatt
     1921 ttctgatcta gtcactggtt tattcaacca tgccgatagt ctggagggcg tgtctgaaac
     1981 ggtttcaact tctttacaaa ctcggactat tcgaaccctg gcactggctc aaatcttaag
     2041 tggcgtaggt gtggctggca cggtagcagc aggatcactt ctcgttactt caatgacaaa
     2101 ttccgaaacg ttagctggca ttgcgcaaac tttcagcgtt tttggagctg cagtaatggc
     2161 gctagttcta gcaaatctga ctagacgtgg cggccgtcga ctggccctat cggcaggtta
     2221 tttcatcggt gcaattggcg ccgcaattgc agtcatcgcc ggaaatagcg cactagtccc
     2281 gttggttttt ctaggcacat ttatgatcgg agcctcttca gccgctggat atcaagcacg
     2341 atacgcagcg atcgatttgg ctgacgacaa aaatcgtgca agtagtttgt ctttcgtagt
     2401 ttgggcttca acaattggat cggtgcttgg tcccaattta cttgaaccac tcggacaggt
     2461 ggcaagccaa tttaatttac ctgaactaac cggaccattt ttaatggccg ccctttcttt
     2521 atttgctggt tcaagtttaa tttggcttcg ccttcgacca gatccctact taaccagttt
     2581 ggctggaacg aaagaagtcg gggccgctac gccagtcaaa atacctttta gtgaaacatt
     2641 tggacatata agatcaaatt caaaatctct actcggtatc tcggcgatcg ctatcggtca
     2701 cgttgccatg gtttcaataa tggtgatgac ccctgtgcac atgaaacatg ttgatgtatc
     2761 gcttcgagta atcggtttag taattagtgt gcacattgct ggtatgtacg cattttcacc
     2821 gctagttgga aaactttccg acaaaattgg ccgagtaagt gtgattgcga tcgctggctt
     2881 agtgctttta tcctctgcag tcattgccgg tttagcctca gcaagcaaca gcactcaact
     2941 cgggatcgga cttttcttac ttggccttgg gtggtccggc actctaatcg ccggatcaac
     3001 tttgctatct gaaaccgtaa gtgatcatta ccgggcatca gctcagggtg tttccgactt
     3061 aatcatgaac ttatctggcg catttggtgg cgcgattgct ggcgtaatta tcgccacttt
     3121 aagttatggc tggttatgcg ccctggctgc cattccagta ggtgtaatga cattgagagc
     3181 gattatcgcc aggaaaaata gcttaataac tgggtaatta ttttggttgc tgctgggtaa
     3241 tgcaatgaat tcccccgcct ctagcaaaaa tctcacgagc atcaactaaa actactttgc
     3301 gccccgggta tgcctgctcc attatttttt tagcgtgctg atcgactgga tcagcaaagg
     3361 cgcaaagaat tacaccacca ttgcagacgt agtgattgat gtaactgtaa tcaacgaatc
     3421 cctcgtcgtc acgaatcact tcaggagctg gcactggaat taacttaaac ttcgtattgc
     3481 ttaaggttgc ttcaacttct ttactaactt caaaatctgg gtgtgctggg tttaactgtt
     3541 ggtgaaacaa aattgtgtcg ggattacaaa aggtagccac aatatcaacg tgtcccttgg
     3601 tgccataatc gtcatagtca cgcgttaaac cacgttcgag ccaaaccaca ttatttgcgc
     3661 ttaaggtccg agcaaactct gcttcaacat cactggctgt ccatcctgga ttacgaccag
     3721 gatctaactg cacagttttt gtgaccaata cagttccagc gccattaacg tgaataccgc
     3781 caccttcatt agttaatttt gaagaaatgc aaggtatttg agtttcctga agtatgtaat
     3841 ttgcaatttg gctgtcatga tcccacttag cccaatgttg accaccccac ccgttaaata
     3901 cccagtcaac accatgaata tttccagctt catctctaac aaaagttggc ccgatatcgc
     3961 gcatccaggc atcatctaag tttgcgggta gtaactcaat tcgattatcc agccattttt
     4021 ttgcggctga aatattctgc ggatcaacaa gcattgtgac tggttcaaat tcaataattg
     4081 catttgcgac atttgcccag gcttcgtagg caatttcaat atctgaatcc gttcctgctg
     4141 agtatgtggc gaccggccac gccatccagg tacgttcgtg aacttcccat tcagctggca
     4201 tccgataagt catgttggca agaatactaa ctagtttctt cgaccggaac ttcgcgcatc
     4261 accaacatca cgcctaatcc agctaaagcc attccaaaaa gtccagacgg ttcaatccgt
     4321 tcatcaaata aggcaaacgc cataattgca gctgatggtg ggaccaggta ataaagactt
     4381 gaagcttttt gtgcactgcc cttcctgagc agagcgtaaa gaattgcgat tgacgctatc
     4441 gaattagcaa tcactgccca agaaagtcca aaaataaatt caggattcca agtaactgaa
     4501 gtattttcca aaactaatgc caaaatcaga aaaacaattg cactggcaaa atactggacc
     4561 gttgtgcccg ttaaaaacgg gatgtttccg ccagttttct tctgcgccag atagccacca
     4621 gtagttccta gtaaggccac aaaacaagta actattcctg ctgtggtgaa atttgtagat
     4681 acatcacctt ggaatattcg aggcaaaagc aaaattgcaa cgccgataat acctaaacca
     4741 agccctacta tctgaattct gcgcaaccgt tcaccaagga ttggtacagc caaaattcca
     4801 actaacaccg gctgcagact aactattaca gcgctcactc cagaactgac gcctaggctc
     4861 acggcaagga agattccgcc gatgtatatc acgtgcagca tcatggctac agcaattgaa
     4921 ctccagaatt gcgatttagt gagtcttaat ttttccttag ctatcagggc tgcaattagc
     4981 agtatcactg cagcaatcgt gtagcgataa gttaaaaatg taaacggttc tgcgtagggc
     5041 ttgatgtagc gagcagcaac aaatccagta ctccataaaa aaacaaaaag taatggataa
     5101 atgcgatctt gtgtcttact aatttaaaac tccccaagta agagcatttt tttaattttc
     5161 aatctcgcca acaattccaa aatttttcgc gtctaactgt ccttgcgagc gatacaactc
     5221 taactttgcc ctggtatcag caatgtctaa attacgcata gttagttggc cgattcgatc
     5281 agctggacca aaagctgcat tttcagtgcg ctccattgat aatttttccg gatcgtaact
     5341 taaagctggt cctttggtgt ccaaaattgt gtaatcatcg ccccgacgta atctcaaggt
     5401 aacttcaccc gtgaccgcag aagcgaccca cttttgcaaa gattcacgca acataagtga
     5461 ttgtggatca aaccagcgac cctcataaag tagccgacca agccttctgc cctcagcatg
     5521 gtaattagcg atcgtgtctt cattgtgaat tgcgctaact agtcgttcat aagcaatgaa
     5581 tagtaaagcc atacctggag cctcataaat accacggctt tttgcttcga ttatgcgatt
     5641 ttcgatttgg tcggccatcc caagtccatg acgaccacca atttcatttg cttctttaat
     5701 tagtgcaaca ttgtttgcaa aagttttacc attgatcgca acaggaaaac cttgattgaa
     5761 agcaattgtt acgtcctcgg ctgagatttt tactgactca tcccaaaagc gaacacccat
     5821 gattggctgc acaatttcca tggattcatt taagaattcc aaagttttag cttcatgagt
     5881 cgcgccaaga atatttgcat cggttgaata agctttctcg acactgtcgc gatatggaaa
     5941 actatgtgcc actaaccatt cggacatttc tttacggcca ccaagctcac gtacaaagtc
     6001 ctggtctaac caaggtttat aaattcgaag atttggattt gctagcaagc catagcgata
     6061 aaagcgttca atgtcgttac ctttataggt tgaaccgtct ccccaaattt caacattgtc
     6121 ttcgagcatc gctcggacaa gtaaagttcc ggtaactgct cgtccaagtg gggtggtatt
     6181 aaagtactga cgaccccctg atcgaatatg gaaggcccca cacgctatcg cagctaaacc
     6241 ttcagcaact aggggttctt tacagtccac aagtcgagaa atttctgcac cgtatgagat
     6301 tgctcgagcg ggtactgaat caatgtcagg ttcgtcgtat tgccctaaat ctgcggtgta
     6361 agtgcacgga atcgcgcctt tgtcgcgcat ccaagcaaca gcaactgacg tatctaagcc
     6421 gccactaaat gcgattccta ctctttcgcc gataggcaat gaggccagca cttttgacat
     6481 gcctaaatct tatcggggct gtctcagatt aaattccaag cttcaatcta gctttgatta
     6541 atttacgaat caaagtcttt gataacggtt tatcgtttgg gaaatgcaag gcacttttag
     6601 tctggctcca gtcagccaat tcaggaaact gacttaacac tgatccgctg tatggataaa
     6661 aactacatga gtttttattt ccggcaattc cagccacacc tttgccgtta attgtgaagg
     6721 taggaattcc gtacttaata cactgctctg cctcaggaat aatttctagg atccgctgtc
     6781 taactacctc aagcgtttgt ctctgagcgg gatcaagttt ggcaagcact tgatcaatat
     6841 cttttgcgct caccagttat ttttagcaca taaagtgaat tcttaagtga aatagaatat
     6901 aaaaatcgtg aataaaaatc catacgaaga agaagcccgt gaacgatggg gcgacacaga
     6961 tgcataccga gactcggcta ctcgcttaaa gagttactct gcagaagacc tagagatggc
     7021 aaaaaaggaa atgcgtgaag ccacgttaat aattcttgat gctatgttgg ctggacttcc
     7081 agccgattcg ccagaggcca tggctggagc tgcagcacat cgaaaatcaa tttcagattg
     7141 gtggtacgac tgtagctacg aaatgcatgt gaacttagcg caaatgtatg ttgcagatga
     7201 aagattttcc gagttttatg aacaaataca ttcagggtta tcgcagtatg tgtacgatgc
     7261 aatttttgcc aacgctttgg caaacagcta actagttttt ctaatcattc gttgatagaa
     7321 catgcctggc ttagatttac tcggtacagg tttaccaact gagacaaatc ctagagcctg
     7381 atatgcagct atagccgagt gattcgtagt ccaaacccct aatccttgaa cttgccatga
     7441 tttactttgt gcatttaagt caacgtagtc aaacatcttt cgcataattc catgaccacg
     7501 aaattctggt ttaacccagc agccacctat ccaacatgtt gccccaaagt ctccggctaa
     7561 attttcaact gtcatcaagc caacatcttt attacgcaaa gttgcgacta ctggagttat
     7621 ctgtccgaat tgactttccc actcggattg cgtttgttgc attgattgct ctaaattgcc
     7681 tccaaaggcc tctggatcag tttttaggct agctatcctg atttcccgaa atctttgcca
     7741 gtccttgaca gataacaact ctggaacgac atggattgca gttaagtccg ccaaatcagt
     7801 ccttttcgtt gatctgcaga aatctagatc ctggggttaa atggaaatat gcaattgaat
     7861 gaaattgatc taaccatgct agacggcaag aaaacaactt ttggtaacta ctccgacaaa
     7921 gttgtgttga tcgtcaatgt tgcttccagg tgtggcctag ctccacagta cgaaactctt
     7981 gaagctatgc aaaagaagta cggtccgcga gggttcactg tgcttggctt accttcgaat
     8041 cagtttctac aagagttgaa tagcagcgaa gcaatatcaa actactgttc aactacatgg
     8101 ggtgtaactt ttccggtcac tgaaaaagtc aaagttaatg gccgcaatca acatccgctt
     8161 tatcgcgaac taacaaaatc taaagatgcc agtggtaagt ctgggcgcat tatgtggaat
     8221 tttgaaaagt ttttagtgct gccaaacggt gaggttcacc gttttcggcc aactacaaaa
     8281 ccagatgatc cggcaattat tgaactaatt gaaaagaatt tgccggctta acctctgcca
     8341 acaactctct tacccgtgga ataacttggg tgccataaag ttcaagcgac ttcatgatct
     8401 ggctttgtgg cagtggacca tttacatatt taaaatcaaa acgttgcgct cctactgaac
     8461 tcaatgcacg agcaattttg cgcgatacag tttcaggcga gccaacatac aaagatccag
     8521 agttaacctc attcaaataa tgctgctttg agaccggact ccagccacgt tctcgcc
//
LOCUS       DBNHVN010000045         1071 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_380392, whole genome shotgun sequence.
ACCESSION   DBNHVN010000045 DBNHVN010000000
VERSION     DBNHVN010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_380392"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..149)
                     /locus_tag="AC16Y1_00815"
     CDS             complement(<1..149)
                     /locus_tag="AC16Y1_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HIR5831036.1"
                     /translation="MNQVFPPNFKWGVATSAFQIEGATKVDGRGETNWDEFCRQPGKV
                     LNGDTG"
     gene            417..>1071
                     /locus_tag="AC16Y1_00820"
     CDS             417..>1071
                     /locus_tag="AC16Y1_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5831037.1"
                     /translation="MTTTKSWQKAAVLAAAAALFLTACGNGGSEEAATSADPKCAPYA
                     DVTGLEGTEVTIYTSIVDPEATLFQQSYADFEECTGITIVWDGSKEFEAQLPVRVEGG
                     NAPDLAIFPQPGLLQAMVATGKMVAAPAAVEANVDEYYGADWKSYGTVDGTFYAAPLG
                     ANVKSFVWFNPSVFAANGWAIPTTWDEMLTLSDTIAASDSGVQPWCVGFGSGDATGWV
                     "
BASE COUNT          275 a          249 c          256 g          291 t
ORIGIN      
        1 ccggtatcgc cgtttaggac ttttccaggc tggcgacaaa attcatccca attagtttcg
       61 ccacggccat caaccttggt tgcgccttct atctgaaatg ccgaggtggc cacaccccat
      121 ttgaaattgg gcggaaaaac ctgattcatg tgcctccttg ggctgctttt gagtctaaag
      181 ggggaattcc tatataaata aggctttttg gattaccaga gttgctaata tgttcgacta
      241 aatctgttat aaatgccgca aaaaccttga tattaaaagg tttttctgaa tgtaatcgtt
      301 acacagtttc agcagtttac tagctgttca ccttgcataa gccgttagtt caatgcaccc
      361 tttactttag ggattcggcc gaatctcagt cattaaaaaa tcggagatat atcagaatga
      421 ctacaacaaa atcgtggcaa aaagctgcgg tgcttgcggc agctgctgca cttttcctta
      481 ccgcatgcgg taacggtgga agtgaagaag cagcaacttc tgcagatcct aagtgcgccc
      541 catatgcgga cgtaactggt cttgaaggca ccgaagttac catttacaca tcgattgttg
      601 acccagaagc aactcttttc caacagtctt atgcagactt tgaagagtgc accggaatca
      661 caatcgtttg ggatggttcg aaagaattcg aagcccaact acctgttcgt gttgaaggtg
      721 gaaacgctcc agatctagcg atcttccctc agccaggtct attgcaggca atggttgcaa
      781 ccggcaagat ggttgctgct ccagctgcag ttgaagctaa cgtagatgag tactacggag
      841 ctgactggaa gtcatacgga acagttgacg gtacgttcta cgctgcacct cttggcgcaa
      901 acgttaagtc attcgtttgg tttaacccaa gcgtcttcgc agcaaatggt tgggcaattc
      961 caactacttg ggatgaaatg cttacccttt cagacacaat cgcagctagc gactcgggcg
     1021 ttcagccatg gtgtgttggt ttcggttcag gtgacgcaac cggctgggtt g
//
LOCUS       DBNHVN010000046         1138 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1569033, whole genome shotgun sequence.
ACCESSION   DBNHVN010000046 DBNHVN010000000
VERSION     DBNHVN010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1569033"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="AC16Y1_00825"
     CDS             complement(<1..592)
                     /locus_tag="AC16Y1_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA family protein"
                     /protein_id="HIR5831038.1"
                     /translation="MALQVMAAPNEPDLLFLPWSHLLEQWPDEATVSLPKGISRHIVK
                     FVRLNGVVYAIKETQQPIAEREYELLQRLVRRGVPCVEPVAVISGREDRDGAELPAAL
                     MTRHLEFSLPYRALFSSTLKPDTAIRLLDALALLLVRLHLVGFSWGDCSLSNTLFRRD
                     AGAFAAYLVDAETGTLEGKLSDGQRDYDVDLAVTNVT"
     gene            complement(624..>1138)
                     /locus_tag="AC16Y1_00830"
     CDS             complement(624..>1138)
                     /locus_tag="AC16Y1_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005272274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HIR5831039.1"
                     /translation="VTHDQVEAMTMGDRVAVLKDGILQQVDTPTNLYNHPVNAFVAGF
                     IGSPAMNLLDLKFDDAGVVIEGAHIPLPSAALTAAKAEGLSEITVGFRPEDLVEDANG
                     FAVTIDLVEVLGADAYVYGSVKDAGGTERQITVRVDGRNTPAKGSTIKLTPTAGHLHV
                     FSTRTGNRLG"
BASE COUNT          322 a          283 c          249 g          284 t
ORIGIN      
        1 cagtcacatt ggtgaccgct aaatcaacat catagtcacg ttggccatca gaaagtttgc
       61 cttctaacgt gccggtttcc gcatcaacta agtaagcagc gaaagcacca gcatcacgcc
      121 taaacaaagt attagaaagt gagcagtcac cccagctaaa gccaactaga tgtaagcgca
      181 ctaataacag cgcaagtgca tcaagcaggc gaatcgcagt gtctggtttg agcgttgatg
      241 aaaacagtgc tcgataaggc aacgagaact ctaaatgccg agtcattaat gcagctggca
      301 gttcggcgcc atcgcgatct tcgcggcccg agataactgc aacaggctca acacacggaa
      361 ctccgcggcg aactaaccgt tgcagtaact cgtactcacg ttcggcgatc ggttgctgtg
      421 tttctttaat cgcataaacc acgccattaa ggcgaacaaa ctttacgatg tgccgactaa
      481 taccttttgg caatgaaact gtagcttcgt ctggccattg ttcgagtaag tgactccacg
      541 gcaaaaacaa taagtcaggc tcattcggag ccgccattac ttgtaatgcc attaagaacc
      601 tatttggtaa ttaattttgc tgattaacct aaccggttgc cagtgcgagt actaaagacg
      661 tgtaagtgac ctgcagttgg agtcaactta attgttgaac ctttcgcagg cgtattacga
      721 ccatcaacgc gaacagtaat ttgtcgttcg gtaccaccgg cgtcttttac tgaaccgtaa
      781 acataagcat cagcaccaag tacttcgact aaatcgattg tcaccgcaaa accatttgca
      841 tcttcgacta ggtcctcagg gcggaagcca actgtgattt cactcagacc ttctgctttt
      901 gcagctgtta gtgcagcgct tggcagcggg atgtgtgcac cttcgattac tacacctgca
      961 tcatcaaact taagatcaag caagttcatc gcaggtgaac caataaagcc agccacaaat
     1021 gcattaactg gatggttata aagattggtt ggcgtatcta cttgttgcaa aatgccgtct
     1081 ttcagcacag caactcgatc acccattgtc atagcttcaa cttggtcgtg ggttacat
//
LOCUS       DBNHVN010000047         1148 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1866342, whole genome shotgun sequence.
ACCESSION   DBNHVN010000047 DBNHVN010000000
VERSION     DBNHVN010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1866342"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="AC16Y1_00835"
     CDS             complement(<1..290)
                     /locus_tag="AC16Y1_00835"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5831040.1"
                     /translation="MVTENQVRKVARDCSLVFNALGDPNRQDILIMLETEGPLNVLQI
                     TERLNLARPTISHHLKILSNAGLLKSTKKSREIFYESEWSDASKNLKKFMKLL"
     gene            609..>1148
                     /locus_tag="AC16Y1_00840"
     CDS             609..>1148
                     /locus_tag="AC16Y1_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5831041.1"
                     /translation="MFFTYLRRELNQRKRQTLLVAIALGTSIGLIIVVNAASAGIGDA
                     QKTVLSGLYGIGTDISVTKSVQPTQGGQSFNFGPGGPQAGGATALNQTRLTVPPFSGT
                     FDQNSLTQITSTKGVAKASGTLRLSQIVFKGSIASGTEPQATPTVPQDPNAAGRRGFG
                     GGNFNVDMTSIEGVDPNEIT"
BASE COUNT          333 a          229 c          247 g          339 t
ORIGIN      
        1 agaagtttca taaatttttt gagattctta gaggcatcag accactcaga ctcgtagaag
       61 atctcccgag acttcttggt cgatttgagt aggccagcat tgctcagaat ttttaggtgg
      121 tgggagatag tcggacgagc cagattcagc cgttcagtaa tctgtaggac attgagcggc
      181 ccttccgttt caagcatgat caagatatct tgcctattgg ggtcgccaag ggcattaaaa
      241 actaatgagc aatccctagc aacttttcga acctgatttt ccgtaaccat aagataaggg
      301 tatcaacccg tcgaaactct tcaaccgaat ccaaattgaa cttttggaag ttgttacaag
      361 ttgattaatc tttggggcaa aattgagggc aattgaacat tttgagccta aattcacgca
      421 caagaatgtc gtctccggct tatagggcga ctcttcttta ttattatttt gttactcaaa
      481 atatttgcaa tattagcact tgatttaatt ttaatattca cggtgaaatc taaggaaaaa
      541 tcgttcacac cttcaggctc aatggcatag actgttaacc tattaaccca atggctcatg
      601 gaggccccat gttttttact tacctgcgca gagaattaaa tcaacgtaaa cgtcagactt
      661 tattagttgc cattgcgttg ggaacttcaa ttggtttaat catcgtggtt aatgcagctt
      721 cggcaggaat tggagatgca cagaaaacgg ttctgagtgg gctttatgga attggcactg
      781 atataagtgt tactaaaagc gtgcaaccaa ctcaaggtgg acaaagtttc aattttggtc
      841 caggtggtcc tcaagcaggt ggtgcaactg cacttaatca aactcgctta acggttccac
      901 cattctctgg aacttttgat caaaattcgc tcacccaaat tactagtaca aagggagttg
      961 ctaaagcttc aggcaccttg cgcttatctc aaattgtatt taaaggaagt attgcttctg
     1021 ggaccgaacc tcaagcaact ccgacagttc cacaagatcc aaatgcggct ggccgtcgag
     1081 gatttggtgg tgggaatttt aatgttgata tgacaagcat tgaaggtgtt gatccaaatg
     1141 aaataact
//
LOCUS       DBNHVN010000048         3708 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1940760, whole genome shotgun sequence.
ACCESSION   DBNHVN010000048 DBNHVN010000000
VERSION     DBNHVN010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1940760"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /gene="dop"
                     /locus_tag="AC16Y1_00845"
     CDS             <1..1454
                     /gene="dop"
                     /locus_tag="AC16Y1_00845"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HIR5831042.1"
                     /translation="NPTANPMVLSSQVVNAYADLTMPNRIKRMRWDYDVESPLRDARG
                     FDMSRADADPSQLTDEDYGLANVVLHNGARFYVDHAHPEYSSPEVTNPLDAALWDAAG
                     DRIMQLSAFHASKLTGELITAYKNNTDGKGVSYGTHENYQLSRSTPFGRIVKYLTPFF
                     VTRCVFAGAGRVGIGQESLVPGFQLSQRADFFEAEIGLETTLRRPIINTRDEPHANPD
                     EYRRLHVIVGDANMSQFANYLKLGTTSLVLSMIEDDYLNVDLDLHDPVSYMHVVSHDY
                     EFKENLTRRNGKTITALEIQEEFIAQAYKYVKDRSKDDPMTMDVLTNWQRVITTLRND
                     PMSLSNELDWVAKFNIMNAYRVRDQALWNDSRIETVDIQYADLRQDKGLAMVLQKKGR
                     MRTLFSEDQISHAISNPPEDTRAYFRGRCVANYEQKIAAASWDSIIFDFGVDQPMVRL
                     NTVDARKGTKELTGDLFDRQLSADEFVSALSAS"
     gene            1511..1693
                     /locus_tag="AC16Y1_00850"
     CDS             1511..1693
                     /locus_tag="AC16Y1_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012245440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031386 - protein tag activity [Evidence
                     IEA];
                     GO_function: GO:0070628 - proteasome binding [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HIR5831043.1"
                     /translation="MTEREFRSASNAQSDEVAEVILPTAAASSIDVSDILDEIDLVLE
                     SNAEEFVKSFVQKGGQ"
     gene            1702..3069
                     /gene="pafA"
                     /locus_tag="AC16Y1_00855"
     CDS             1702..3069
                     /gene="pafA"
                     /locus_tag="AC16Y1_00855"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HIR5831044.1"
                     /translation="MSVHQRRIYGIETEFGVTCTFEGTRRLTPDEVARYLFKRVVAWG
                     RSSNVFLTNGSRLYLDVGSHPEYATAECDDISQLVIHDRAGERVVEDLIADAQERLVH
                     EDIAGQIYLFKNNTDSSGNSYGCHENYLIRRRADYLTQIEALLPFLISRQLISGAGKV
                     LETPRGTIYSLSQRADHMWEGVSSATTRSRPIINTRDEPHADSESYRRLHVIVGDSNM
                     CEMTTMLKVASMEFVLRMLESGFLKRDFTLDNPIRAIRDVSHDITGRGLITLSSGQSL
                     TALELQTEYLRLAKEFSAQEEFNPTLKRSLELWERALTAIETGNYSLIEKDIDWAIKK
                     KLLDSYMEKNSLDLTSPRIAQLDLMYHDITQSRGLFYMLERKGLANRISTDDQVQEAK
                     TVPPQSTRAKLRGDFISAAQAKNKDISVDWVHLKMNDQTQRTVLCKDPFLNVDERVNR
                     LIDAM"
     gene            3102..3635
                     /locus_tag="AC16Y1_00860"
     CDS             3102..3635
                     /locus_tag="AC16Y1_00860"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR5831045.1"
                     /translation="MIKKLAVIILSTTIVVACGANEAEINPAPSETSATQDQSPLDPI
                     SISGVEISGNSGSAPVIKVLEVGSVVDQLQIADQKVGVGTAVPAGATVLAHYVGYGLT
                     TGLMFQSSWDLGSPIQFGLDQVIAGWGEGIPGMKIGGRRILVIPAELAYGQNPPPDSG
                     IQPGEALLFVIDLVDFI"
BASE COUNT         1092 a          743 c          876 g          997 t
ORIGIN      
        1 caaatccaac cgcaaacccg atggttctct ccagccaagt ggttaacgct tacgctgact
       61 taactatgcc taatcgcatt aagcgtatgc gatgggatta tgacgttgag tcaccgttac
      121 gcgatgcccg tggttttgac atgagtagag cagatgctga tccaagtcag ttaactgatg
      181 aggattacgg attagccaac gtagttctgc ataatggagc gcgcttttat gtagatcatg
      241 ctcatccaga atattcctca cctgaagtta caaatccgct cgatgctgcc ctttgggatg
      301 ctgcgggaga tcgcataatg caattaagtg catttcatgc cagtaaattg accggtgaat
      361 taatcacggc atataaaaat aacaccgatg gtaaaggagt tagttatggc acgcatgaga
      421 actatcagct atctagatca acaccattcg gtcgaatagt taaatattta acgccatttt
      481 ttgtaactcg atgcgtgttt gctggtgctg gacgtgttgg cattggtcaa gagagtttgg
      541 tcccaggttt tcagttatca cagcgcgcag atttctttga agccgaaata ggtcttgaaa
      601 cgacgcttag gcgcccaatt attaatacac gcgatgaacc tcatgctaac cccgatgaat
      661 atcgacgact acatgtaata gttggtgacg ccaacatgtc acaatttgca aactatttga
      721 aacttggtac aacctcgctc gtcctgagca tgatcgaaga tgactatctg aacgtagatc
      781 tcgatttaca tgatccagtt tcatatatgc atgtagtgag tcacgattat gagtttaagg
      841 aaaatttgac gagacgtaat ggaaagacga ttacggcttt ggaaattcaa gaagaattta
      901 ttgcgcaggc ttataaatac gtgaaagatc gaagtaaaga tgatccgatg actatggatg
      961 tcttaactaa ttggcagcga gtgattacta cgctacgtaa tgatccgatg agtttgtcga
     1021 atgaattgga ttgggtggct aaatttaaca ttatgaatgc ctatcgagtt cgtgatcaag
     1081 ccctttggaa tgattcacgg attgagactg tcgacattca gtatgcagat ttgcgacagg
     1141 ataaagggtt agcaatggtt ctccagaaaa agggccgaat gcgaacactg tttagcgaag
     1201 atcaaatatc ccatgcaatt tcgaatccac ctgaagatac ccgcgcttat tttcgtgggc
     1261 ggtgtgtggc taattacgag cagaaaattg ctgcagcgtc ctgggactcc ataatttttg
     1321 attttggtgt ggatcaacca atggtccgac ttaataccgt agatgcgcga aaaggcacta
     1381 aagaattaac tggtgactta tttgatcgac aactatcggc cgatgagttt gtgtccgcgt
     1441 taagtgcgag ttagatctaa cataggcttt aagcaaggat actttcgggc taaagaggta
     1501 ggcagtacaa atgactgaac gcgaatttag gtcggcaagt aacgcacaat cagatgaagt
     1561 cgcagaagtt atcctgccaa cggctgcggc aagcagtatt gatgtttcag acatactcga
     1621 tgaaattgat ctggtcctcg aatctaatgc agaagaattt gttaaaagtt tcgtgcaaaa
     1681 aggtggacag taaattacac gatgagtgtt caccagcgcc gaatttatgg cattgaaact
     1741 gagtttggcg tcacctgcac atttgaagga actcgccggc taactccaga tgaagttgct
     1801 cgttatttat ttaagcgcgt agttgcatgg ggaagaagca gtaatgtttt tttaacaaac
     1861 ggctcaagac tttacttgga tgtcggaagt catccagagt acgccactgc tgaatgtgat
     1921 gacatttctc agttggtcat ccatgatcga gcgggcgagc gggtagtcga agatctaatc
     1981 gcggatgcgc aagaaagatt ggtccacgaa gatattgctg ggcagattta tctattcaaa
     2041 aacaacacag attcaagcgg aaattcatac ggatgtcatg aaaactattt gatcaggcgt
     2101 cgtgctgatt atttaaccca gatagaagcg ctattaccat tcttgattag ccgccaatta
     2161 attagcggcg ctggaaaagt tttagagact ccgcgaggaa ctatatattc tctttctcag
     2221 cgggcagatc acatgtggga aggtgtatcg agcgcaacca cgcgttcacg ccccattatt
     2281 aatactcgtg atgagccaca tgcagattca gaaagttatc gacgactaca tgtaattgtc
     2341 ggagattcca acatgtgcga aatgacgacc atgctaaagg tcgcttcgat ggaatttgtt
     2401 ttgcgcatgc tggaaagtgg ttttctaaag cgtgacttta ctttagataa tccaatacgt
     2461 gcaatcagag atgtctcgca cgacatcacc ggtcgcggtt taatcacgct tagttctggt
     2521 cagtcgctaa ctgcactcga acttcaaact gaatatttgc gactagccaa agaattcagc
     2581 gcgcaagagg aatttaatcc gacacttaag cgatctttag agctttggga aagagcacta
     2641 actgcaattg agacgggtaa ctactcgcta attgaaaagg acattgattg ggctataaag
     2701 aaaaaacttc ttgattcata catggagaaa aattcactcg atttaactag tccgcggatt
     2761 gcgcagttag atctgatgta ccacgacatc acccaaagtc gcggcttgtt ttacatgtta
     2821 gagagaaaag gtttagccaa cagaatttct accgacgatc aggtacaaga agccaaaact
     2881 gttccaccac aatcaactcg agcaaagcta cgcggtgatt ttatttccgc agcgcaggca
     2941 aaaaataaag atatttctgt ggactgggtg catttaaaaa tgaatgatca gactcagcga
     3001 acggttttgt gtaaagatcc gttcttaaat gttgatgaac gagtcaaccg cctcatagac
     3061 gcaatgtagt tcctgaaaat gaatactgat aaagtcgcac catgatcaaa aaattagcgg
     3121 tcatcattct ttcgacgaca attgtggttg cttgtggtgc aaatgaagct gagataaacc
     3181 cagctccgtc agaaacaagc gcgactcagg atcagtctcc attagatcca atctcaatct
     3241 ctggcgtaga aatcagtgga aactcaggta gtgcacctgt gatcaaagtg cttgaagtcg
     3301 ggtctgttgt tgaccaactg caaattgccg atcagaaggt tggtgtcggc acagccgtac
     3361 ctgcaggtgc aaccgtgctg gcgcactatg ttggctacgg attaacaact ggattgatgt
     3421 tccagtcatc gtgggatctc ggttcgccaa tacaatttgg tttagatcaa gttattgccg
     3481 gatggggcga aggtattccg ggaatgaaaa taggcggccg tcgaattttg gtaattccag
     3541 ccgaacttgc ttacgggcaa aatccaccac ccgatagcgg aattcaaccc ggtgaagctt
     3601 tgctttttgt tattgattta gtcgatttta tttaggagaa ccgtgaataa gccagaagta
     3661 gatttcaatg agggtccggc acccaaggaa ttaaacataa aagacatc
//
LOCUS       DBNHVN010000049         1609 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_602169, whole genome shotgun sequence.
ACCESSION   DBNHVN010000049 DBNHVN010000000
VERSION     DBNHVN010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_602169"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..308
                     /locus_tag="AC16Y1_00865"
     CDS             120..308
                     /locus_tag="AC16Y1_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831046.1"
                     /translation="MNQNPNEGDQKDLDTKDKVRNLKLRALLDGLDLTPEQTKDDLAK
                     PSNRDSEIIADVPPHHGN"
     gene            complement(305..961)
                     /locus_tag="AC16Y1_00870"
     CDS             complement(305..961)
                     /locus_tag="AC16Y1_00870"
                     /inference="COORDINATES: protein motif:HMM:NF020250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HIR5831047.1"
                     /translation="MSPKSICAVISTLMQTKIQLFLIRYRREISATLAAIGILLLISI
                     IRAATPTIYAITTVRDLPAGHKITSSDLSSKKIPSSLTWPTLLTQTESVIGKVTSHAL
                     ATGTPLSNSDLISSDLLTGFASNQIAISIPISGNRVDAYLTSGNHINVYATQNGDPAK
                     LVEYDAVVLFVPPNKSGGFQISSSSDNSIILAVNQGESAAISTYIGNGTFSFALLPNN
                     "
     gene            1003..1314
                     /locus_tag="AC16Y1_00875"
     CDS             1003..1314
                     /locus_tag="AC16Y1_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004947092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR5831048.1"
                     /translation="MPTYQYECESCGNPLEVVQSFSDDVLTICPSCNEPKLKKVYGSV
                     GVVFKGSGFYKTDSRSSGSTTAPTTSTPSSTGGTATPTTSPSAPAPAASPAPAKTSTP
                     K"
     gene            1317..>1609
                     /locus_tag="AC16Y1_00880"
     CDS             1317..>1609
                     /locus_tag="AC16Y1_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter FluC"
                     /protein_id="HIR5831049.1"
                     /translation="MKKLGLIAALGAVIGSLLRYFVGSLVNSTSFPWATFTVNVVGSL
                     LIGLFLTLPIITQHEPRRVFLITGVLGGFTTFSALAVESLQLSAHLALLYIFSS"
BASE COUNT          463 a          333 c          345 g          468 t
ORIGIN      
        1 cagacgaaac tcgatcaaat tccgggcgcg taacttcaac atcccaaagt acgccaccta
       61 tttgcacgat tacctcgatc gattaggaaa tttgtcgctt aagtatcagc ataggagttg
      121 tgaatcagaa tcccaatgaa ggtgaccaaa aagatttgga tacaaaagat aaggtgcgaa
      181 atttaaaact tcgtgcactc cttgatggtc ttgacttaac accggaacaa actaaagatg
      241 acctagccaa accaagtaat cgagatagcg agattatcgc tgatgtgcca ccacatcatg
      301 gcaattagtt atttggcaaa agtgcaaaac taaaagttcc attaccgata taagttgaaa
      361 ttgcagctga ctcaccttgg ttaaccgcca aaattattga gttatcgctg ctacttgaaa
      421 tttgaaagcc gccagattta tttggtggca cgaataaaac cactgcatca tattcaacta
      481 gttttgctgg atcaccattt tgagtggcat aaacatttat gtgatttcca cttgttagat
      541 aggcatccac tcgatttcca gaaatgggaa ttgaaattgc gatttggtta ctggcgaacc
      601 cagttaataa atctgaacta attagatccg aattactaag tggggttccg gttgcaagtg
      661 catgacttgt tactttgccg attacagact cggtttgcgt gagtaaggtc ggccaagtta
      721 gcgaactagg aatcttctta cttgataaat cgctcgatgt tattttgtgc ccagctggta
      781 aatcccgaac tgtcgtgatg gcgtaaatcg taggggtagc cgctcggatg attgaaatta
      841 aaagcaggat tccgatggcc gccaaagtgg ctgatatttc gcgccgatag cggattaaaa
      901 ataactgaat tttggtttgc ataagagtgg aaattacggc acaaattgat tttggcgaca
      961 ataaatttcc acaggtacga ttacctaggt tgaaaggacg atatgcctac ttatcaatat
     1021 gagtgtgaat cttgtggcaa tccactcgaa gtagtgcagt ccttttctga tgatgtacta
     1081 actatttgcc catcatgtaa cgaacctaag ttgaaaaaag tttatggttc agttggtgtg
     1141 gtgtttaaag gctctgggtt ttataaaaca gattcgcgta gttcgggttc aaccactgcg
     1201 ccgacgactt ctacaccttc atcaactggc gggacggcaa caccaactac atcgccgagc
     1261 gctccggcac ctgcagcaag tcctgcaccg gcaaagactt caacacctaa ataattttga
     1321 aaaaactagg acttattgct gcacttggcg cagtcattgg ttcattactt cgatacttcg
     1381 ttggatcttt agttaatagc acaagtttcc cgtgggccac ttttacagtg aacgtagttg
     1441 gctctttatt aatcggttta tttttaacgc tcccgattat cacgcaacac gaaccacgcc
     1501 gagtattttt aatcactggt gtacttggtg gattcacaac tttttcggca ctggctgtag
     1561 aaagtttgca gttaagtgca cacctggctt tgctttacat tttcagttc
//
LOCUS       DBNHVN010000050         1076 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1643808, whole genome shotgun sequence.
ACCESSION   DBNHVN010000050 DBNHVN010000000
VERSION     DBNHVN010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1643808"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="AC16Y1_00885"
     CDS             <1..264
                     /locus_tag="AC16Y1_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831050.1"
                     /translation="SFVMDLADPKVKAAVKDSAKVTLFIAGDNDHEWTPRGLTETFGK
                     PLDLPSVVIPGAGHMTVEQSPSWGHWQGVIDWVNDPTASLLKK"
     gene            275..>1076
                     /locus_tag="AC16Y1_00890"
     CDS             275..>1076
                     /locus_tag="AC16Y1_00890"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5831051.1"
                     /translation="MDQIWHDLKKLWHLKPFRTLFLARVISNFGNGLAPVAIAFGVLG
                     LPGATATDLSKVQAALFFPLVVFMLIGGIVADRFPRALIIGSADILLSIFVIASGILF
                     LTDQQTVTKLIIISFISGTLNALWWPAFMGLTPEIVDEDNLQSANSVIAFGANSMNIF
                     GIVAGGALVASIGAGLAILIDGITFLIAGLFVLRLRKFGKTRKSDEHSPTVIEDLIHG
                     WKEFSTRSWVVTVVLGYTVIAMAFESIFAVLGPYNAKLNLDGARPWSWI"
BASE COUNT          283 a          189 c          248 g          356 t
ORIGIN      
        1 agttttgtga tggatttggc tgatccaaaa gtaaaagcag cagttaaaga ctctgccaaa
       61 gtgaccttat ttatagccgg tgataacgat cacgaatgga cccctcgtgg cttaactgaa
      121 acttttggca aacctttgga tttaccatcc gtagtaatac ctggcgctgg tcacatgacg
      181 gtagaacaaa gtccttcgtg gggtcattgg cagggcgtaa ttgattgggt aaatgaccca
      241 accgcaagtc tgcttaaaaa atagagcgtg aagtatggat caaatttggc atgacctaaa
      301 aaagttatgg catttaaaac catttcgcac acttttctta gctcgcgtta tttctaactt
      361 cggcaatggc ttagctcccg tagccattgc atttggcgta cttggtttac cgggagccac
      421 agcaacagat ttaagtaaag ttcaagcggc attatttttt ccattagtgg tttttatgtt
      481 gattggcggc attgttgctg atcgctttcc ccgagcatta attattggaa gtgcagacat
      541 acttctaagt atttttgtta tcgcaagtgg aatcttattt ttaactgatc aacaaactgt
      601 aactaaattg attatcattt cgtttatttc aggaacacta aatgcgcttt ggtggccagc
      661 atttatggga ttaactccgg aaatagtcga tgaagataat ttgcagtcgg ctaattcggt
      721 gattgcattt ggcgctaatt caatgaacat ttttggcatt gtggccggtg gcgcattagt
      781 tgcctctatt ggggcaggtc tagcaatttt gattgacgga attacttttc ttattgctgg
      841 tttgtttgtt ttacgtttac gtaagtttgg taagacgcga aaatcggatg agcactcacc
      901 aactgtgatt gaagatttaa tacatggttg gaaagaattc agtactcgta gttgggtagt
      961 aacagtagta cttggttaca cagttattgc gatggcattt gaatcaattt ttgcggttct
     1021 tggtccatac aatgcaaagt tgaatttaga tggcgctcgg ccttggtctt ggattt
//
LOCUS       DBNHVN010000051         1241 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1569364, whole genome shotgun sequence.
ACCESSION   DBNHVN010000051 DBNHVN010000000
VERSION     DBNHVN010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1569364"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..>1241
                     /locus_tag="AC16Y1_00895"
     CDS             163..>1241
                     /locus_tag="AC16Y1_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017956313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5831052.1"
                     /translation="MNTEFDVIIVGSGFGGSVAALRLSEKNYHVLVVEAGKEFKDHEY
                     AKTSWRLRKFLFAPALRCFGIQRIHRLPNVLVLAGAGVGGGSLVYANTMYEPMEEFFA
                     QGPWAKITNWQKELKPFYDLARRMLGVVENPVFTPADKAMQSVAQRMGVGATFRLAPV
                     AVHFGQKPGELYDDPYFGGIGPARKGCTNCGECMTGCRHNAKNTLDKNYLALARKLGA
                     QIQAMTTVTKIKQDGDDWVVSTKRTGGFKKQTFKAKHVVVAAGTYNTQKLLHQMKNTG
                     NLPNISPRLGYLSRTNSEALLGAVSDKVQEPDFTNGVAITSSFFLETQTHIQPVRYGV
                     GSNAMGLLATVFTDGDDNVARWRIW"
BASE COUNT          358 a          245 c          289 g          349 t
ORIGIN      
        1 gcgtcgacat ggcgttgaag gtttaatgcg attcaccgaa tcacaaacga ttgccgtaca
       61 tccaactttt ataactggcc cgcgttttgg atttaccgag cagcgctgga atatgttgat
      121 atcgcgggca cttagattgt taaagaaact cggacttcgt taatgaatac agagttcgac
      181 gtaataattg ttggctctgg tttcggcgga agtgttgcag cgcttcgatt aagtgaaaag
      241 aactatcacg ttttagttgt tgaagctggt aaagaattta aagatcatga gtatgcaaaa
      301 acatcttggc ggcttcgtaa gttcttattc gcaccggctc tgcggtgctt tggaatacag
      361 cgcatccaca gattgcccaa tgtcttagtt ttagctggag ccggagttgg cgggggatca
      421 cttgtttatg caaacactat gtatgaacca atggaagaat tttttgctca aggcccttgg
      481 gcaaagatta ctaattggca aaaagagtta aaaccttttt acgacctagc cagacgcatg
      541 cttggcgttg ttgaaaatcc agttttcacg cctgcagaca aagcgatgca gtcagtggcg
      601 cagcgaatgg gagttggagc tacttttcgt ttagctccag ttgccgtgca ctttggacaa
      661 aaaccgggtg aactttatga tgatccctat tttgggggaa ttggaccagc ccgcaaaggt
      721 tgcacaaact gcggtgaatg tatgactggc tgtcggcata atgcaaaaaa tactttagat
      781 aaaaattatt tagcgttagc ccgaaagctt ggcgcccaga ttcaagctat gacaactgtg
      841 acaaaaatta aacaagacgg tgatgattgg gttgtttcta ctaaacgaac tggcggattt
      901 aagaaacaaa cttttaaagc aaaacatgtt gtagtcgcag ccggaactta caatacgcaa
      961 aagttacttc atcaaatgaa aaacactgga aatttaccaa acatttcacc gcgacttggg
     1021 tatttatctc gcaccaattc cgaagcactt ttaggtgccg tatcagacaa agttcaagaa
     1081 ccagatttta cgaatggtgt tgcaattact tcctcattct ttctagaaac ccaaactcac
     1141 atccaaccag ttcggtatgg agttgggtca aatgcaatgg gcttgttagc tacagttttt
     1201 actgacggag atgacaatgt tgctcgttgg cgaatttggt t
//
LOCUS       DBNHVN010000052         1436 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1420847, whole genome shotgun sequence.
ACCESSION   DBNHVN010000052 DBNHVN010000000
VERSION     DBNHVN010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1436)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1420847"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..539)
                     /locus_tag="AC16Y1_00900"
     CDS             complement(<1..539)
                     /locus_tag="AC16Y1_00900"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR5831053.1"
                     /translation="MASPLSVVLLGGGTVGSAVAQLLVEQAEDLAARIGRPLELKGVA
                     VRDANKSRSGIAPALITEDALKLASSGADIVIELIGGIEPAKTLIETAIANGSSVVTA
                     NKALLAAHGAQLHKLAEENGVDLYYEAAVAGAIPLIRPLRESLAGDKVKKILGIVNGT
                     TNFILSKMYSDGADYNSVLA"
     gene            complement(630..>1436)
                     /locus_tag="AC16Y1_00905"
     CDS             complement(630..>1436)
                     /locus_tag="AC16Y1_00905"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005480288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase family protein"
                     /protein_id="HIR5831054.1"
                     /translation="EDQKFGLSIASGAALAAIDSVIAAPALSLLGLHSHIGSQIFDAS
                     GFEIATHRVIQFAALVREKFDFTVPEINVGGGMGIAYVESDDPLNVEHMAKAIHDIVT
                     AECHEANFPIPRLAVEPGRAIIGGAAVTVYEVGTVKPVDVSDELTRNYLSVDGGMSDN
                     IRTALYDARYTVTLASRSSSGVAHTARVVGKHCESGDIVVRDTQLPADIAPGDLLAVA
                     ATGAYCRAMASNYNYLPRPAVVSVKGGQTQVLLRRETLEDVLSLDPGITN"
BASE COUNT          398 a          346 c          312 g          380 t
ORIGIN      
        1 gccaaaacgg aattgtagtc agccccatct gaatacattt tgctcaaaat aaagtttgta
       61 gtgccattta caatgcccaa aattttctta actttgtcac cagcaagtga ctcgcgtaac
      121 gggcgaataa gcggaattgc gccagcaact gctgcttcgt aataaagatc aacgccattt
      181 tcttcggcta gtttgtgtag ctgggcgcca tgtgcagcaa gtagagcttt gtttgccgtt
      241 acaaccgatg agccatttgc aatcgctgtt tcgataagag tcttagctgg ttcaatgcca
      301 ccaattaatt cgatgacaat gtcggcacca gaactggcta acttcagtgc atcttcggta
      361 ataagagccg gtgcaattcc tgatctagat ttattagcgt cgcgaactgc aacaccttta
      421 agttccagcg gtcgaccaat tcgagcggct aaatcttcgg cctgttcaac aagtaactga
      481 gcaaccgcag agccaaccgt gccgccacca agcaggacaa cagatagtgg ggatgccatc
      541 agtactccta cttagtgcta tttcatacct tgcagtcttt acagtgttat aagtctggca
      601 gaacagaact atccgaccaa aattactgat taatttgtga tacctggatc tagtgaaagt
      661 acatcttcta gagtttcacg gcgaagtaaa acttgggttt ggccaccttt aacgcttact
      721 actgctggcc taggtaagta gttgtaattg cttgccattg ctcgacagta agccccagtc
      781 gcggcaactg cgagtaaatc tccgggagct atatctgcag gcaattgggt atctcgaaca
      841 actatgtcac cgctctcgca atgtttgcca acaacgcgag cggtgtgagc cacaccagaa
      901 gaacttcgtg atgcaagtgt gactgtgtat cgcgcatcgt aaagagcagt tctgatgttg
      961 tcactcatcc caccatcaac actcaggtaa ttacgagtaa gttcatcaga aacatctacg
     1021 ggtttaacgg tgccaacttc atatacagtt acagccgcac caccgattat tgctcgacca
     1081 ggttctactg ccaacctcgg aatcggaaaa ttagcttcgt ggcattcagc agtaacgatg
     1141 tcgtgaattg cctttgccat atgttcaaca tttagtggat catcactttc gacataagca
     1201 attcccattc caccaccgac gtttatttcc ggaacagtaa aatcaaattt ttctcgaacc
     1261 agggctgcaa attgaattac tcgatgtgtg gcaatttcaa aacctgaggc gtcaaatatt
     1321 tgcgagccga tgtgtgaatg taatccaagt aatgaaagtg ccggcgcggc aataaccgag
     1381 tcgatcgcag ctaaagctgc gccagaagca attgaaagtc cgaacttttg atcctc
//
LOCUS       DBNHVN010000053         4701 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_900736, whole genome shotgun sequence.
ACCESSION   DBNHVN010000053 DBNHVN010000000
VERSION     DBNHVN010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_900736"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="AC16Y1_00910"
     CDS             complement(<1..1008)
                     /locus_tag="AC16Y1_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase beta subunit"
                     /protein_id="HIR5831055.1"
                     /translation="MKVFVPADSSAISVGAEEIAESLVELGAQVIRTGSRGMFWLEPL
                     VEVETEKGRVGYANVEPADVAKLLSNDLASKSIGLVDEFDWLKKQNRVTFARVGVIDP
                     VSVADYEAHGGLVGLRKALAMTSEQIVEEVTQSGLRGRGGAGFPAGIKWKTVLNTYAD
                     QKYICCNADEGDSGTFADRMLMEGDPLTLVEGMIIAGLAVGATTGLIYCRSEYPHAID
                     VLNRAIKNAYEQGVLGKSVCGSHFSFDLEVRSGAGSYVCGEETAMLESIEGKRGMIRS
                     KPPIPAISGLYGKPTVINNVMTLSAVPMILADGAASYQELGVERSRGTQVFQLAGNIA
                     RG"
     gene            complement(1005..1478)
                     /locus_tag="AC16Y1_00915"
     CDS             complement(1005..1478)
                     /locus_tag="AC16Y1_00915"
                     /inference="COORDINATES: protein motif:HMM:NF013426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HIR5831056.1"
                     /translation="MTQFVVDDLTAQLEEFRGDGYAVLKALQKTQDMLGFVPDSALDV
                     VAKVCNVSRAEVYGVFTFYSDFRRVAPAKCVVKVCVAEACQANGSRELISQLHDQGFD
                     VHKATRVGEVQMEQTFCLGNCALGPAALVNGVPVARATAKKLIEKANNAASGISR"
     gene            complement(1475..2065)
                     /locus_tag="AC16Y1_00920"
     CDS             complement(1475..2065)
                     /locus_tag="AC16Y1_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HIR5831057.1"
                     /translation="MSLAVVACGAIASHVHDIAARRNWDLHVEPVNPLLHNRPEGIAP
                     EVDALLGKLKNDFETVVVAYADCGTYGELDKVCQKHEVNRLAGEHCYDVFATAERMRK
                     EFEAEPGTFVLTDYLVKSFRRSVMKELGLEKYPELRDDYFHSYRRILWLAQHQTQELI
                     DAAHDVAHYLKLPLEIIQVGDQYLEEQLVELVGESK"
     gene            complement(2062..3924)
                     /locus_tag="AC16Y1_00925"
     CDS             complement(2062..3924)
                     /locus_tag="AC16Y1_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HIR5831058.1"
                     /translation="MSDQKVEPTEIAAGLGRVTVNFTPSGQEIRVPPGVSVFDAASWN
                     GLAIDSTCGGHGTCKKCKIQVLEGEVPVSRLDIRAFTPEQLKDGWRLACVARANSNLK
                     IDVPPLATRPKAATVGVGRQVILRPGVQKRYAELVEPSLHDQRTDIDRLRDAMEDLEF
                     EADLNVLRVLPTVLRKAEYKVTAVVVGEKLIAVEPGDTSEQNYAIGIDLGTTTVVATL
                     LDLVTGTPMAVKSMLNKQQPFGADVISRISATMMDPQAGPLMQKAALDTFNELIDEVC
                     TEAKVDKNQIYALSIAGNATMVQLLLGVDPEPVGVAPFITASQRWPIMTAGEIGIDVL
                     PSARCYTFPSFGAYVGGDIVAGVLATGMDRDKRTRLLIDVGTNCEIVLSNGDTILSTA
                     APAGPAFEAASIACGMRAADGAIEVVQIKDGELVLGVIADATPIGICGSGLVDAVYAL
                     HQVGLIDDSGKFVTDEQAAQIAPKIAARLVTRPEGDRVFNLFGEGSSAVYLSQRDVRE
                     LQFAKASIATGWRLLLEELNLTDDDVQQVLLAGSFGSYLSPTSAVGIGLVPKLPVLRI
                     VSAGNVAGEGAKMVLLSDTERHGAEALLDEVKYVELSDRSDFQEKFFVELALPS"
     gene            complement(3957..>4701)
                     /locus_tag="AC16Y1_00930"
     CDS             complement(3957..>4701)
                     /locus_tag="AC16Y1_00930"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR5831059.1"
                     /translation="QEKLRNDDLSTINIDVADQVAGGADMLDVNMGVPLTDEPVLLAK
                     AIKLVQSLTDLPICIDSSVIEALDAGLAAYEGKALVNSMTGEDDRMDAILPLVKKYDA
                     AILALPNDETGIPMTADERMPIVEKIVRRVEKEGISLENLLIDPLAMPVGAEPEAVKQ
                     TLETIYRVREKYGLNMSIGASNVSFGLPNRHALNASFMPMAMSMGLTSAIMDARTPEV
                     VQSVRAADLLLALDAWGSNWITNFRESKS"
BASE COUNT         1279 a         1211 c          932 g         1279 t
ORIGIN      
        1 acctcttgca atgttgccgg caagttggaa aacctgcgtt ccacgtgaac gctcaacgcc
       61 aagctcctga taactggccg cgccatctgc caaaatcatc ggcacggctg aaagagtcat
      121 cacgttatta atgacagttg gttttccata taagcctgag attgccggaa tcggtggttt
      181 tgatctgatc attccgcgtt tgccttcgat gctttccaac atcgcggtct cttcaccaca
      241 aacatacgag ccagcaccac tgcgaacttc taaatcaaaa gaaaaatgtg aaccgcaaac
      301 ggattttcct agcacgcctt gctcgtatgc atttttaatg gctctattca gaacatctat
      361 tgcatgtggg tactcagaac gacaataaat taatccggtg gtggcgccga ctgctaatcc
      421 agcaataatc atgccttcaa ctaaagtgag cgggtcacct tccatcagca ttcgatcggc
      481 aaaagttcca ctgtcacctt cgtctgcgtt acaacaaata tatttttgat ctgcataagt
      541 atttaaaaca gttttccact taatgccagc tgggaatcct gctccacctc gaccacgtaa
      601 acctgattga gttacttctt caacgatttg ctccgaagtc attgccagtg ccttgcgtaa
      661 accaactaag ccgccgtgag cttcatagtc agctactgaa actggatcaa tcacaccaac
      721 gcgggcaaaa gttactcgat tttgtttttt tagccaatca aattcatcta ctaaaccaat
      781 agatttactg gctaggtcat ttgaaagtaa ttttgctaca tcagctggct ctacattggc
      841 atagccaact ctgccttttt cggtttctac ttcaaccagt ggctcaagcc agaacatgcc
      901 acgcgaacca gttcgaatta cttgtgcacc aagttcaact agtgattcgg caatttcttc
      961 ggcaccaacc gatattgcac tcgaatctgc tggaacaaaa actttcatcg gctaatccca
     1021 ctcgctgcat tattcgcttt ttcaatcagt ttttttgctg ttgcccgagc aactggaaca
     1081 ccgtttacca gcgcagctgg accaagcgca caattaccta agcagaaagt ttgttccatc
     1141 tgcacttcac cgacacgagt agctttatga acatcaaaac cttgatcatg taattgcgaa
     1201 attaattcgc gactaccatt tgcctggcaa gcctcagcaa cacaaacttt gacgacgcat
     1261 tttgctggcg caactctgcg aaagtcacta tagaaagtga acacaccgta cacttcagcg
     1321 cgtgagacgt tacaaacctt agccaccaca tctaatgctg aatctggtac aaaacccaac
     1381 atgtcttgag tcttttgcaa agccttcaag actgcgtatc catcgccacg aaattcttct
     1441 agctgggccg ttaaatcgtc gaccacaaat tgtgtcactt tgactccccg actagttcca
     1501 ccagttgttc ttccaagtat tggtcgccca cctgaattat ttcaagtggc aactttagat
     1561 aatgtgccac atcatgagcc gcatctatca actcctgtgt ttgatgctgg gctagccaca
     1621 aaatacgccg atatgagtgg aaatagtcat ctcgtagctc tgggtatttt tccaagccaa
     1681 gttctttcat cacactgcgg cgaaaggact taactagata atccgtcaaa acaaaagtac
     1741 ctggttctgc ctcaaactct tttcgcattc tttctgcagt ggcaaatacg tcataacaat
     1801 gttcaccagc taaccgattc acttcgtgtt tttggcaaac cttatcgagc tcgccataag
     1861 ttccacagtc tgcataagcc acgactactg tttcaaaatc gtttttaagt ttgcctagta
     1921 gcgcatcaac ttcaggcgca ataccttcag gtcgattgtg tagtaacgga ttaacgggtt
     1981 caacatgcag atcccaattg cgtcgtgctg caatgtcatg tacgtgactg gcaatcgcac
     2041 cacaggcaac tacggcaagg ctcatgaagg cagcgccaat tcgacaaaga acttctcttg
     2101 gaaatctgac cggtctgaga gttctacata tttaacttca tctagcaaag cttcagcacc
     2161 atgacgttca gtgtcagaaa gtaaaaccat tttggcacct tcaccggcga catttccagc
     2221 actaacaatt cttaaaaccg gaagttttgg cactaaacca attccaacag ctgaagttgg
     2281 tgaaaggtaa gaaccaaaag atccggctaa cagaacctgt tgcacgtcat cgtcagttag
     2341 attcaactct tcaagaagta gccgccagcc agttgcaatt gaagctttcg caaactgaag
     2401 ttcgcgcaca tcacgttgag atagatagac agcgctactg ccttcgccaa atagattaaa
     2461 aactcgatcg ccttccggcc tagtcactaa tctggcggca atttttggtg cgatttgtgc
     2521 tgcttgttca tccgttacaa attttccaga gtcatcaatt agacctactt gatgtagcgc
     2581 atagacggca tcaactagac ctgaaccgca aatacctatc ggagttgcat ctgcaatcac
     2641 tcctaggact aattcgccat ctttgatttg cacaacttca atcgctccgt cagccgcgcg
     2701 cataccgcat gcaatcgatg cagcttcgaa tgctggacct gcaggtgctg cagtagaaag
     2761 aatcgtgtcg ccatttgaca aaacaatttc acaatttgtg ccaacatcaa taagtaagcg
     2821 agttctctta tcacggtcca tcccagtggc tagaacccca gcgacgatgt caccgcctac
     2881 gtaggctcca aatgatggga atgtatagca ccgagcactt ggcaatacat caatgccaat
     2941 ttcgccagca gtcataattg gccacctttg ggatgcagta ataaatggcg caaccccaac
     3001 cggttcagga tctacaccaa gcaacaactg gaccatcgtt gcattgcccg cgattgaaag
     3061 tgcgtaaatt tgattcttat cgactttggc ctcggtacaa acttcatcaa ttaattcatt
     3121 gaaagtatca agagcggctt tttgcataag cgggccagcc tgtggatcca tcatggtcgc
     3181 cgaaatccgc gaaataacat cagcaccaaa cggctgctgc ttatttagca tcgacttaac
     3241 tgccatcgga gtaccggtaa ctaaatcaag taatgtcgca acgactgtgg ttgtacccag
     3301 gtcaatccca attgcgtagt tttgttcact ggtgtcgcct ggttcaacag caattaactt
     3361 ttcaccaacg acaacagcag tgaccttgta ttcagctttg cgtaacacag ttggaagcac
     3421 ccgaagcaca tttaaatctg cctcaaattc cagatcctcc attgcgtcac gtaatcgatc
     3481 aatgtcagtt cgttgatcat gtagcgaggg ttcaactaat tcggcataac gtttttgcac
     3541 ccctggacgc aaaataactt ggcgaccaac tccaactgtg gctgcttttg gcctggttgc
     3601 aagtggcgga acgtcaattt ttaaattgct atttgctctg gcaacacacg caaggcgcca
     3661 accatctttt aactgttctg gagtgaaagc tcgaatatcc aagcgggaaa ctggtacttc
     3721 accttcaagc acctggatct tgcatttctt gcaagtacca tgtccgccac aagttgaatc
     3781 aatcgctaat ccattccaag atgcagcatc gaatacgctg acacctggtg gcacgcgtat
     3841 ttcttgccca cttggggtga aattaactgt tacccgcccg agaccagccg caatctcagt
     3901 tggttcaact ttctgatcgc tcacagtttc cttagttagt tttggttaag acaactttat
     3961 gattttgatt cacggaagtt agtaatccag ttactacccc aggcatcaag tgccaagagt
     4021 aagtcagctg ctcttaccga ctgcactact tctggcgtac gagcatccat aatcgctgaa
     4081 gttaatccca tagacatagc catcggcatg aatgatgcat ttagtgcatg gcgatttggt
     4141 aacccgaacg aaacgtttga tgcgccaata gacatgttta ggccgtattt ttcacgcact
     4201 cgataaatag tttctagagt ttgtttcact gcctctggtt cggcaccaac tggcatagcc
     4261 agcggatcga ttaacagatt ctcaagcgag ataccttctt tttcaacacg acgcacaatt
     4321 ttttcgacaa tcggcatacg ctcatccgca gtcattggga ttccagtttc atcgtttggt
     4381 agcgccaata ttgctgcgtc atacttttta actaacggca aaatcgcatc cattcgatca
     4441 tcctcgccgg tcattgaatt tactaaagct ttgccttcgt aagcagctaa gccagcatca
     4501 agtgcttcaa tgactgatga atcgatacag attggtaggt cagttaacga ctgcacaagc
     4561 ttgatggctt ttgccaaaag cactggctca tcagttaacg gcacacccat gtttacgtcg
     4621 agcatgtcgg caccaccagc aacctggtct gcgacatcaa tatttattgt tgatagatca
     4681 tcgttgcgca atttttcttg g
//
LOCUS       DBNHVN010000054         7963 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1421434, whole genome shotgun sequence.
ACCESSION   DBNHVN010000054 DBNHVN010000000
VERSION     DBNHVN010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 7963)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7963)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7963
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1421434"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="AC16Y1_00935"
     CDS             <1..534
                     /locus_tag="AC16Y1_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831060.1"
                     /translation="VGATAEYRYGTVDHTYLVAPKRSRVLIAKLLVHALIGAIAGLIS
                     FGIAFATTTVSVSGKDHATVATSTLVEIGIATVAAFAIYTMLGVAFGSLVRNQLVGVA
                     ATFGWIFIFERLMVVFTPDLGKWLPGGAVNGLFGVTAIFNGEEYLPRNQSAWLLLTYA
                     TTLIVASLIITPKRDIN"
     gene            complement(535..975)
                     /gene="rpiB"
                     /locus_tag="AC16Y1_00940"
     CDS             complement(535..975)
                     /gene="rpiB"
                     /locus_tag="AC16Y1_00940"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HIR5831061.1"
                     /translation="MRIAVGSDHAGFHLKNTLRDWLVNQGHEVEDVGTFSAERADYPI
                     YGAAAAAKVANGDAEFGVVVCGSGQGICMAANKVAGIRGAVIRDTQDAQMSRQHNDAN
                     VACFGERVTESDAAIDALQVFLDTSFEGGRHQNRVDLLGELDQK"
     gene            complement(989..1897)
                     /locus_tag="AC16Y1_00945"
     CDS             complement(989..1897)
                     /locus_tag="AC16Y1_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5831062.1"
                     /translation="MSEISVKSWLPLWTVISITWGFSFLFIKVAGEFLDPFQQTFGRL
                     ALGAIVVSMFMLVTKRRFISDKKAIAHLAFLAVIAHAIPFTLVAWAEHYITTIAASLI
                     NSMLSLWVALFAVWFLPEEKLNRTKFYGLLLGFFGIMILLGVWDASFRGNWFAYGAGV
                     LSTVGYAAANIWTRKNITPMKLDPFSATAAQLAIGATIMGLVSLGVSTKPTSYPFNGV
                     LSLVLLGIVGTGIAFALNLELINRAGSVISSTTGYSMPVVATVAGVIFLREELNWYEP
                     IGAVIALVGIAITQGLIKSRRSSKTS"
     gene            2142..2417
                     /locus_tag="AC16Y1_00950"
     CDS             2142..2417
                     /locus_tag="AC16Y1_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HIR5831063.1"
                     /translation="MSADNSGGIPERDQRILEFERQWWKFAGAKEQAIREQFDMSATR
                     YYQVLNSLIDQPEALAFDPMLVKRLQRMRAARQRARSARRLGIELNK"
     gene            2427..2903
                     /locus_tag="AC16Y1_00955"
     CDS             2427..2903
                     /locus_tag="AC16Y1_00955"
                     /inference="COORDINATES: protein motif:HMM:NF024791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HIR5831064.1"
                     /translation="MSGGQLKKRIIVPLIVISGSVAGLAFVGVQLLGLSGANDEITQT
                     MPSISASPTQTVAAPSPTPTEIPGVVERLPVIVLNGTDRAGYAKQIGDNLVFENWVIQ
                     EVGNWDGDLFLQNMVFYPAGYEAAATELALSTNVNGMIAPAEGDFSQTALTVVLAQ"
     gene            2904..3302
                     /locus_tag="AC16Y1_00960"
     CDS             2904..3302
                     /locus_tag="AC16Y1_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003983962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5831065.1"
                     /translation="MIRIQCLTIDCHNPKLLGEFWGEVLGWQVTHESETEVVVEPPAG
                     SPLVNVAPDILFLKVPDKKVVKNRLHLDLRPDDRDAEVARIKKLGAVEVEIGQSQDPN
                     VTWVVLADPEGNEFCVLQPPQGKSTTAFFN"
     gene            3391..3672
                     /locus_tag="AC16Y1_00965"
     CDS             3391..3672
                     /locus_tag="AC16Y1_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010985363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR5831066.1"
                     /translation="MSVTVRIPTMLRSYVDDQSRVEITPQENTLRSVFAALEEMAPGI
                     LQRVVSDSGELRRFVNVYVDDDDVRNLAGVDTPTPDGCAVSIVPAVAGG"
     gene            3768..4898
                     /locus_tag="AC16Y1_00970"
     CDS             3768..4898
                     /locus_tag="AC16Y1_00970"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5831067.1"
                     /translation="MSWFIFGMGPAFALLREDLGVTRTVIAFHNVAASIGSILAGVTS
                     TYLINRFGRGNMIRTGAIGMIIGLLLFTTGPTIAITLPSMLICGYSGILIIQCNSAFM
                     NNHHGKAAPSVISEVNALGALVGFMAPIVLGIGIAAGFGWRIGLGSIVIAFIVIEIVR
                     GKDTSAFGSRKSSETSHDHDEPGALPKKFWTAWLALAFTTAIEASILTWGSELLKTQS
                     GLNTATATAAVGTIVLGMGIGRFFGARLMQTYDVEKLYRQSLLGAFIAFLAFWQGTNQ
                     FVQLTALGLMGLIMSVHFPLGITRLMRASLGRPDRAAAMSSIGSGAAAGFVPFLVGAT
                     ADSTTIIFAFIIPAAALAMAFLLAVRYSTPVTNEPIEAEIGL"
     gene            5129..5329
                     /locus_tag="AC16Y1_00975"
     CDS             5129..5329
                     /locus_tag="AC16Y1_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR5831068.1"
                     /translation="MLGTVKWFNQEKGYGFIEVDGTDKDVFVHYSSIETDGFKTLDQD
                     QRVEFEIGDGPKGPQAEHVKPA"
     gene            5432..5755
                     /locus_tag="AC16Y1_00980"
     CDS             5432..5755
                     /locus_tag="AC16Y1_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5831069.1"
                     /translation="MAWFYLVLAIIFELAGTTALKFSDGFTKLYWTLGMAVSYGFAFY
                     LLSIVVKTIPLGIAYAVWAGLGTLGAFLISIWVFKSEASGLAWFGVVLVVTGVALLNI
                     GSDAH"
     gene            5936..7561
                     /gene="groL"
                     /locus_tag="AC16Y1_00985"
     CDS             5936..7561
                     /gene="groL"
                     /locus_tag="AC16Y1_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133870.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR5831070.1"
                     /translation="MSKIIAFDEEARRALERGMNVLADAVRVTLGPKGRNVVLEKKWG
                     APTITNDGVSIAKEIELEDPYEKIGAELVKEVAKKTDDVAGDGTTTATVLAQALVREG
                     LKNVAAGSNPMILKKGIEAAVDAISKELLAMAQKVDSKDQIAATASISAADTQIGQMI
                     AEAMDKVGKEGVITVEESNTFGLELELTEGMRFDKGYISPYFVTDPERMEAVLEDPYI
                     LIANSKISAVKDMLPVLEKVMQSGKPLMIIAEDVEGEALATLVVNKIRGNFRSIAVKA
                     PGFGDRRKAMLQDIAILTGAQVISEEVGLKLENTDLDLLGKARKVVVTKDETTIVEGS
                     GDADQIAGRVNQIRAEIEKSDSDYDREKLQERLAKLAGGVAVIKAGAATEVELKERKH
                     RIEDAVRNAKAAVEEGIVAGGGVALLQASEKAFKNLKLEGEEATGANIVKVAVEAPLK
                     QIAINAGLEGGVVAEKVRNLEPGWGLNAATGEYVDLIKAGIIDPAKLTRSALQNDASI
                     AALFLTTEAVIADKPEKTPAMPGGDGGMGGMDF"
     gene            complement(7612..7782)
                     /locus_tag="AC16Y1_00990"
     CDS             complement(7612..7782)
                     /locus_tag="AC16Y1_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831071.1"
                     /translation="MAKEKAYEPNQDDVAEFSGAMDELVAKYDQVISEFTNILDQQTK
                     RIFGDPKLEDKE"
BASE COUNT         2212 a         1786 c         1852 g         2113 t
ORIGIN      
        1 gttggagcaa cggctgagta tcgatacggc accgttgatc acacttactt agtagcacca
       61 aagcgctctc gagttttgat tgcaaaactt ctcgtccatg cgttaatcgg cgcgatcgct
      121 ggcttaattt catttggcat cgcgtttgcc acaacaactg tttcggtatc gggcaaggat
      181 catgcaacgg ttgcgacttc aactttggtt gaaattggaa ttgccaccgt tgctgcattt
      241 gcgatttaca caatgcttgg cgtggcattt ggatcgttag tgcgcaatca acttgttggc
      301 gttgcggcca cattcggctg gatctttatt tttgaacgcc taatggtggt ctttactcca
      361 gatttgggta agtggttacc tggtggtgca gtcaacggtt tatttggcgt aacagccatc
      421 tttaatggtg aagagtatct accacgtaat caatcagctt ggttattact aacttacgca
      481 actacgttga ttgtggcgtc attgattatc acaccaaaac gcgacataaa ttaattactt
      541 ttgatctaat tcaccaagta agtcaacgcg gttttggtgc cgaccacctt caaacgaagt
      601 gtctaaaaat acctgcagcg catcaattgc agcgtcactt tcagttacgc gttcaccaaa
      661 gcacgcgaca ttcgcgtcgt tgtgctgtcg actcatttgc gcatcttgcg tgtcgcgaat
      721 tactgcgccg cgaataccag caactttatt cgccgccata caaatacctt gaccgctgcc
      781 acaaactaca acaccgaact ccgcatcacc gtttgcaact ttggctgcag cagcagcgcc
      841 ataaattggg tagtcagcac gctcagcgga aaaagttcca acatcctcga cttcgtggcc
      901 ttgattaact agccaatcgc gaagtgtgtt ttttaaatga aaacctgcat ggtctgaacc
      961 aactgcgata cgcatgaaac taactttact aagaagtttt gctagaacgt ctggatttaa
     1021 tcagaccttg ggtaatcgca attccaacta atgcaattac ggcaccaatt ggttcatacc
     1081 aattcaattc ttcgcgcaag aaaataacac ccgccacagt tgccacaact ggcattgagt
     1141 aaccggtagt tgaacttatt actgaaccag cgcgatttat taattcaagg tttagggcga
     1201 aggcgattcc ggttccaaca atgccaagta acacaagtga gagcacacca ttgaatgggt
     1261 aactagttgg ttttgtggaa accccaagtg aaacaagtcc catgattgtt gcgccaattg
     1321 ctaattgagc cgcggttgcc gaaaacggat ctaatttcat cggggtaatg tttttgcgcg
     1381 tccaaatgtt tgcagctgca taaccaacag ttgagagcac accggcgcca taggcaaacc
     1441 agttaccgcg gaaacttgca tcccaaacac caagcaaaat catgatgccg aaaaaaccga
     1501 gtagtaaacc ataaaacttt gtccgattta gtttttcctc gggtaaaaac caaacggcaa
     1561 ataatgcaac ccaaagtgag agcattgagt taattaaact tgccgcaatt gtggtgatgt
     1621 agtgctcagc ccaagcaact aaagtaaacg gaatcgcatg tgcgataaca gctagaaaag
     1681 ctaaatgcgc gatcgctttt ttgtcgctta tgaaccttct ttttgtgacc aacatgaaca
     1741 tgcttacgac aattgcgcca agtgccaggc ggccaaatgt ctgttgaaat gggtctaaaa
     1801 actcgccagc aactttgatg aataaaaatg aaaagcccca agtaatcgaa atcacggtcc
     1861 agaggggtaa ccaacttttt actgagattt cagacacgtt tcacagtctg tcgtattggg
     1921 tttacgggat atcttccgcc ccatatgtca cacctctcac atatgtttca attaagactg
     1981 ttgagttgaa tgatttggtg cagatgaata acgagcaggg ggacgaaatt atgtttgatg
     2041 ccccgcatgc tgtgcaaaat gacaccccga gcgagggggt taccacgggt attactgact
     2101 ctgcctttaa cattgatgat gtgagtgaag ccctacgtgc tatgtctgca gataattcag
     2161 gtggaatacc agaacgcgat caacgcattc ttgaattcga acgccaatgg tggaaatttg
     2221 ccggtgctaa agagcaagca atccgtgaac agtttgatat gtcggctact cgctactacc
     2281 aagttttaaa ttcacttatc gatcaaccag aagcattggc tttcgatcca atgttggtta
     2341 aacgcctgca acgtatgcgc gctgctcgtc agcgagcgcg ctcagctcgt cgtctaggta
     2401 tcgaactaaa taagtaaggt ggcccaatgt ctggtggcca attgaaaaaa cgcattatcg
     2461 ttccgttaat tgtcatttcc ggcagtgttg cgggtcttgc atttgttggc gtgcagttac
     2521 ttggtttaag cggagccaat gatgaaatca cccaaacaat gccatcaatc tcggctagtc
     2581 caacccaaac tgttgcggcg ccctcaccaa ctccaaccga aatacctgga gttgttgagc
     2641 gacttccagt tattgtgctg aacggaacag accgtgctgg ttatgcaaaa caaattgggg
     2701 acaatttagt ttttgaaaac tgggtgattc aagaagttgg taactgggat ggtgacttgt
     2761 ttttacaaaa catggttttc tatccagctg gttatgaagc agccgcaact gaacttgctc
     2821 tttccacaaa tgtaaatggc atgattgcgc cagccgaagg tgactttagt caaactgcat
     2881 taacagttgt attggcacaa taaatgattc gcattcaatg tttaacaatt gattgtcata
     2941 atccaaaatt acttggtgaa ttctggggcg aagtattagg ttggcaagtt acccatgaat
     3001 cagaaactga agttgtcgtc gaaccacctg caggctcacc acttgttaat gttgccccag
     3061 acatattgtt tttaaaagtc ccagataaaa aagttgttaa gaatagattg catttagatc
     3121 tgcgaccaga tgatcgagat gccgaagttg cacggattaa aaaactgggc gcagttgaag
     3181 ttgagatagg ccaaagccag gacccaaacg taacttgggt tgtgcttgcc gacccagaag
     3241 gtaacgagtt ttgtgttctg cagccaccac agggtaaatc aacaactgcg ttttttaatt
     3301 aactcaagcc ctgctttcaa ctagaacctg attaagtagc aagataaatt aagcgttgaa
     3361 taacaacagt caaacaactg taggtgccaa atgagtgtga cagttcgaat cccaaccatg
     3421 ctgcggtcat acgtggatga tcaatcacga gttgaaataa ctccgcaaga aaacacattg
     3481 cgaagtgttt ttgccgcgct cgaagaaatg gcacctggaa tcttgcaacg tgtagtcagt
     3541 gattcaggtg aactacgccg ctttgtaaat gtttatgtag acgatgacga tgtgcgcaat
     3601 ttagctggtg tcgatacccc gactccagat ggctgcgcgg tttcaatcgt gcctgctgtt
     3661 gctggtggct gattgctaac taccctggcg tagtcttagt aaccggggcc aaaactaatt
     3721 tcgagcgtga tcgtttaacc aagatcactt acgcccaaat tgcggcgatg agctggttta
     3781 tttttggcat gggtccagcc tttgctctgc tccgcgaaga cttaggtgta acccgcactg
     3841 ttattgcctt tcacaacgta gctgcttcaa ttggttcgat tttggctggc gtgacttcga
     3901 cctacttaat taatcgtttt ggccgcggta acatgatccg caccggtgca atcggaatga
     3961 tcatcggcct attacttttc acaactgggc caacgattgc gatcactttg cccagcatgt
     4021 taatttgcgg atattcgggc attttgatca tccagtgcaa ctcagcattt atgaataacc
     4081 atcacggtaa agcggctcct tcggtgatca gcgaagtaaa tgcccttggc gcacttgttg
     4141 gctttatggc tccgatagtt ctcggtatcg gaattgcagc cggctttgga tggcgcattg
     4201 gcctgggttc gattgtcatc gctttcatcg tcattgaaat agtgcgcggt aaagacacca
     4261 gcgcctttgg ttcgcgtaaa tcaagtgaaa cttcgcatga ccatgatgag cctggcgcat
     4321 tacctaaaaa gttttggact gcttggctag ccctggcctt taccacagcg attgaagcct
     4381 caattttgac ttggggatca gaactattaa aaactcaaag tggcctaaac accgcaaccg
     4441 caactgctgc tgttggcaca atcgtgcttg ggatgggaat cggccgcttt tttggtgcac
     4501 ggctaatgca gacttatgac gttgaaaagt tgtatcgcca atcattactc ggtgccttca
     4561 ttgcattcct tgcattctgg caaggcacaa atcaattcgt gcaactaacg gcacttggct
     4621 taatgggctt aatcatgtcg gtacatttcc cacttggtat cacgcgactt atgcgagcta
     4681 gccttggtcg acctgatcga gctgcagcaa tgtcatcaat tggttccgga gccgcagctg
     4741 gcttcgtccc atttttagtt ggcgcaactg cagattcaac aaccattatt tttgcgttca
     4801 tcattccagc ggcagcactt gccatggcat ttctacttgc agttcgctat tcaaccccag
     4861 ttactaatga gccgattgaa gccgaaatag gcctttaatt aaaaggtttt tgcccaccaa
     4921 tttctgatag ttttcagagg atttataccg tagagttagt atcggtcgaa attaagtaag
     4981 cgtgttagac aaagagtaag actcagttag ggtacgactc agatgattcg cgagcaagat
     5041 agcgaactct cactttagtg ggcagtaagc gtaataaaag gcccgcaggt aacaaaagtt
     5101 atttgcgccc gctaaggaaa gagatgccat gttaggcact gttaagtggt tcaatcagga
     5161 aaaaggctac ggcttcattg aagttgatgg cacagataaa gatgtgtttg tgcactattc
     5221 atcaattgaa actgacggtt ttaaaaccct agatcaggat caacgagttg aattcgaaat
     5281 cggcgatggc ccaaagggac cacaagctga acacgtaaaa cctgcataag accgcagaac
     5341 tcaaaaaggc ccgaggtttc ctcgggtctt tttatttgcc cgtcagcaag ccgcgaatgc
     5401 taggtatcga taaacagggc agaatggata aatggcttgg ttttatttag tgctggcaat
     5461 catttttgag ttagctggca caacagcact taagttttca gatggcttta ccaagctcta
     5521 ttggaccctt ggcatggcgg tcagttatgg ctttgcgttc tatctacttt caatcgtggt
     5581 taaaacgatc ccgcttggta ttgcttatgc agtctgggct ggccttggca ctttaggggc
     5641 cttcctgatc tcgatttggg tgtttaaatc cgaagctagt ggcctggctt ggtttggcgt
     5701 cgtattagtt gtaacaggtg tggctttgct gaatattggc tcggacgcgc actaaaccaa
     5761 aattgcccaa aggcttattt ttagccactt ttacccgatt taggcattct cgatttgcac
     5821 tctaggcccg agagtgccaa caatatgatt agcactcgga cccccttgag tgctaacaaa
     5881 ctaaccagaa ctttggggcc aaaaccccaa ccatttggca aagaggaaaa aacacatgtc
     5941 gaaaatcatc gcattcgatg aagaagcccg tcgcgcatta gagcgtggca tgaacgtact
     6001 cgctgacgca gttcgcgtaa cccttggacc aaagggacgc aacgttgtgc ttgagaagaa
     6061 gtggggcgca ccgactatca ctaacgacgg tgtgtccatc gccaaggaaa tcgaactaga
     6121 agatccatac gaaaagatcg gtgcagaact agttaaagaa gttgccaaga agactgacga
     6181 tgtcgctggt gacggaacta caactgcaac tgttcttgct caagcgctag ttcgcgaagg
     6241 tctaaagaac gtagcagctg gttcaaaccc aatgatcctt aaaaaaggaa ttgaagctgc
     6301 agtagatgcc atcagcaagg aactacttgc catggcacaa aaagtagatt caaaagatca
     6361 gattgctgca actgcatcta tctcagcagc tgacactcaa atcggtcaga tgattgctga
     6421 agcaatggac aaagttggta aagaaggtgt gatcaccgtt gaagaaagca acactttcgg
     6481 tcttgaacta gagctaactg aaggtatgcg ttttgataag ggttacatct cgccttactt
     6541 cgtaactgat ccagaacgta tggaagcagt acttgaagat ccatacatcc tgattgctaa
     6601 ctcaaagatt tcagctgtga aagacatgct gccagtactt gaaaaagtaa tgcagtcagg
     6661 taagccactg atgatcatcg ccgaagatgt tgaaggcgaa gcactagcaa ctcttgtagt
     6721 taacaagatc cgcggtaact tccgttcaat cgccgttaag gctccaggct ttggtgaccg
     6781 tcgtaaggcc atgttgcaag acatcgcaat ccttacaggc gctcaggtaa tcagcgaaga
     6841 agttggtcta aagcttgaga acaccgacct agacctatta ggtaaagcac gcaaggttgt
     6901 agtaactaaa gatgaaacca caatcgttga aggttcaggc gacgcagatc aaatcgcagg
     6961 ccgtgtgaac caaattcgtg ctgaaatcga aaagtctgat tcagattacg atcgcgaaaa
     7021 actacaagaa cgtctagcca agctagctgg cggtgtagca gtgatcaagg caggtgccgc
     7081 aactgaagtt gaacttaagg aacgcaagca ccgtatcgaa gacgctgttc gcaacgctaa
     7141 agctgctgtt gaagaaggta tcgtcgccgg tggtggcgtg gccctacttc aggcttcaga
     7201 aaaagcattc aagaacctaa agcttgaagg cgaagaagca actggtgcaa acatcgtcaa
     7261 ggtagctgtt gaggctccgc taaagcagat cgcgattaac gcaggtcttg aaggcggagt
     7321 cgttgcagag aaagttcgta acttagaacc aggttggggc ctaaacgctg caactggcga
     7381 gtacgttgac ttaatcaagg ctggaattat tgacccagct aagttaactc gcagtgcact
     7441 acagaatgac gcatcaattg ctgcactatt cctaactacc gaagctgtaa ttgcagataa
     7501 gcctgaaaag actcctgcaa tgccaggtgg cgacggtggc atgggcggta tggacttcta
     7561 gtaagttccg taaaacctta aaaaagaccc ggatgaaaat ccgggtcttt tttattcttt
     7621 atcctcgagt tttggatcac caaaaatacg tttagtttgc tgatctaaaa tatttgtaaa
     7681 ttcactaatt acttggtcgt acttagccac cagttcatcc atggcgccgg aaaattccgc
     7741 gacatcatct tgatttggct cgtaagcctt ttctttggcc atgacacatc cgaactattg
     7801 gtcttgagaa tctaaattta gtgccggcgg tttggatacg tctagttttt cagggttttc
     7861 ccactcagta aatgccttac ttgatcctca atctgaattt aattagcaag taactcgtca
     7921 gcatccacaa tgcgatacgc ataaccctgt tcagctaaga atc
//
LOCUS       DBNHVN010000055         2342 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_975424, whole genome shotgun sequence.
ACCESSION   DBNHVN010000055 DBNHVN010000000
VERSION     DBNHVN010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_975424"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="AC16Y1_00995"
     CDS             <1..432
                     /locus_tag="AC16Y1_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5831072.1"
                     /translation="EARMILTEPGDMLNTGVEIEARPPGKTYKRLSLLSGGEQSLTAV
                     AFLVALFKARPSPFYILDEVEAALDDVNLGRLLGILAELRQDSQLLIVTHQKRTMEIA
                     DALYGVSMRGDGVTQVISQRITRDNQAPEAAADLAYAQQVN"
     gene            442..1518
                     /gene="ftsY"
                     /locus_tag="AC16Y1_01000"
     CDS             442..1518
                     /gene="ftsY"
                     /locus_tag="AC16Y1_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017589555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR5831073.1"
                     /translation="MEQTQILGLLLVLVVVGIFIFFKVKKKTKPDLVSQTELRLSQEH
                     SDQIPVGQTEDTQVNSHSVSRNGKLTKSQSGINATFSRLFSNSNISSQDWQELEDALI
                     MADVGAKTSTEIVEQLKKSAKSGSPAQLKQIAKTQLTELVGGNFDRRLKVHRKNDQPA
                     VILTVGVNGTGKTTTTGKLARKLVNQDLDVVLGAADTFRAAAAEQLTTWADRIGVPVI
                     RGGLESDPAAVAFEAVKHGVESQADVVLIDTAGRLHTKQGLMDELGKVYRVASKSTSI
                     DEVLLVIDATTGQNGLNQARVFAEVVPVTGIVLTKLDGTAKGGIVIAVQQELGVPVKL
                     VGLGEGPDDLAEFNPTEFVSGLVD"
     gene            complement(1515..2129)
                     /locus_tag="AC16Y1_01005"
     CDS             complement(1515..2129)
                     /locus_tag="AC16Y1_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831074.1"
                     /translation="MKKILVGVVSLVVLIGLAFALTSGRDRQNNYEPMPGDTAWVQPP
                     SMIDPTLDSYTEVNVGSFVVLRVDDAENWSVEIEDPTLLMFQAGGDQGTYETYPALEA
                     LAEGKTKVIAINGKGEKFEFSVNIKPKDGQLWGPDSMTLALAEEVIGLTEEEAIELID
                     NKSGQIVYRIVKRDGESFPVTMDYRLDRINFEITQGVVTYAYVG"
     gene            complement(2171..2290)
                     /locus_tag="AC16Y1_01010"
     CDS             complement(2171..2290)
                     /locus_tag="AC16Y1_01010"
                     /inference="COORDINATES: protein motif:HMM:NF015419.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831075.1"
                     /translation="MAHLDSSMLRGFVDADGYAVITPGGQEMNSPVLWISLPK"
BASE COUNT          676 a          503 c          519 g          644 t
ORIGIN      
        1 gaagccagaa tgattctgac tgaaccgggc gacatgttga acactggtgt tgaaattgaa
       61 gcacggccac caggtaaaac atataaacga ctttctttgc tatctggcgg tgaacaatcc
      121 ttaactgcgg ttgcgtttct tgttgcattg tttaaggcac gaccaagtcc gttttatatt
      181 cttgacgagg tcgaagctgc actcgatgac gttaacttag gtcgactact tggaattctg
      241 gccgaactcc gtcaagattc acagttgtta attgtcacgc accaaaagcg cacgatggaa
      301 attgctgatg cactttacgg ggtttcaatg cgcggtgatg gtgtaactca agtaattagc
      361 caacggatta ccagagacaa tcaagcccct gaagcagccg ccgatttggc atacgcccaa
      421 caagttaatt aacaggtaat tatggaacaa actcagatac tgggcttgct tctagttcta
      481 gttgttgtcg gaatatttat attttttaaa gttaagaaaa agacaaagcc agatttagtc
      541 tcacaaacag agctccgtct tagtcaagaa cattcagatc aaattccagt tgggcaaact
      601 gaagatactc aggtaaattc tcattcggtt tcacgtaacg gcaaactgac taaatctcag
      661 agtggaataa atgcgacttt tagtcggcta tttagtaact caaatatctc ttcgcaagat
      721 tggcaagagc tagaagatgc attaatcatg gccgatgttg gtgcaaaaac ttcaaccgaa
      781 atagttgagc agttaaagaa aagtgcgaag tctggttcgc cagcgcagtt aaagcaaatt
      841 gcaaaaaccc aattaaccga acttgttggc ggtaatttcg atcgccgctt aaaagtgcac
      901 cgtaaaaatg atcagccagc cgtgatctta actgttgggg taaatggaac tggaaaaact
      961 acgacaaccg gcaaacttgc tcgaaaactt gtaaatcaag atttagatgt agtgctcggt
     1021 gctgctgata cattcagggc cgcagccgct gaacaattaa cgacttgggc agaccggatt
     1081 ggagttccag taattcgcgg tggattggaa agtgatcctg ccgctgttgc gtttgaagca
     1141 gttaaacacg gtgttgaaag ccaggctgat gttgtgctga tagatactgc cggtcgttta
     1201 catactaaac aaggattaat ggatgaactc ggcaaagttt atcgagtggc cagcaagtcc
     1261 acttcgattg atgaagtgct tttagtaatt gatgccacaa cgggccagaa cggattaaac
     1321 caggcgcgag tttttgctga agttgttccg gtaactggca ttgtgctcac aaaactcgat
     1381 ggcaccgcaa aaggtggaat cgtaattgca gttcagcaag aacttggcgt tccagttaaa
     1441 ttagtcggcc ttggtgaagg tcctgacgat ttagcagaat ttaatccaac tgaattcgtt
     1501 tccggtttag tcgactagcc aacgtatgcg taggtaacta caccttgggt tatctcgaaa
     1561 tttattcgat cgaggcggta gtccatagtt accgggaatg attcgccatc gcgtttaact
     1621 attcgataaa caatttgacc cgacttattg tcaattaact cgatagcttc ctcttcagtt
     1681 aatccaataa cttcttcggc cagggccagt gtcatcgagt ctgggcccca tagttggcca
     1741 tctttaggtt ttatgttgac ggaaaattca aatttttcgc ccttgccatt tatggcaatc
     1801 acttttgttt taccttcagc tagtgcttca agagctgggt aagtttcata agtaccttga
     1861 tcgccgcctg cttggaacat caacaaagtt ggatcttcaa tctcaaccga ccagttttca
     1921 gcatcgtcaa ccctcaagac cacaaagctg ccaacattaa cttcggtgta gctgtcaaga
     1981 gttggatcaa tcattgaggg tggctgaacc caagcagtat cgcctggcat tggttcgtaa
     2041 ttattttgcc tatcccgtcc ggaggttagg gcaaaagcta agccaatcag cactaccaaa
     2101 ctaacaacac caaccaaaat tttcttcatg tattgagtat gcctggatag cttttgattt
     2161 actagtttta ctattttggc aaggaaatcc acaacactgg tgaattcatt tcttgaccac
     2221 ccggagtaat gactgcatag ccatcagcat caacaaaacc acgcaacatc gaagaatcaa
     2281 gatgtgccac tggttctact aagccaatat tctgtagacc agttagttgc cttgctaaaa
     2341 cg
//
LOCUS       DBNHVN010000056         5860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_455628, whole genome shotgun sequence.
ACCESSION   DBNHVN010000056 DBNHVN010000000
VERSION     DBNHVN010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_455628"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="AC16Y1_01015"
     CDS             <1..536
                     /locus_tag="AC16Y1_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR5831076.1"
                     /translation="ARLRLLSNQDGTILHDGREVLKRLVTQVSNPVRWDLCMKTMLDL
                     GVTAMIELPPAGTLIGLAKRSMPGVELLALKTPADLPAALDLVSRHSSVSPVGDRPTW
                     RLVVAPYKGIIEFNVSEEPGTELKDNEKVATIRTLRDEYEVTAPHGGSIVEWLVTNGD
                     PVNPGQPLLRLHPRAGA"
     gene            533..1528
                     /locus_tag="AC16Y1_01020"
     CDS             533..1528
                     /locus_tag="AC16Y1_01020"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR5831077.1"
                     /translation="MTKLKTPAPTSHAGLIGLGVYRPSRVVPNSEIVDAIDSSDEWIR
                     ERSGIIERRFAGPAETAASMGAAAAKNAIADAGLTVEQIDVVLVATFSHRYQTPSAAV
                     EVAAEIGAVNASAFDISAACAGFCYGIGLADALIRAGNAKNILVIGSEKLSDITNMSD
                     RGTAFIFADGAGAVVVGPTQEQGIGPTIWGADGTAREAIIMEPDLVTAGKQGVPSLLT
                     MQGQAVFRWAVGSMGDVCQRALDAAEIETSDLKAFVPHQANIRITDAIVKRFNFPDSV
                     VIAKDIQTAGNTSAASIPLALHALREAGQVKKGDPFLMVGFGAGLAFASQVALVP"
     gene            1604..1846
                     /locus_tag="AC16Y1_01025"
     CDS             1604..1846
                     /locus_tag="AC16Y1_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015747320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR5831078.1"
                     /translation="MSQEDILAGLAAIVNEVAGIPAESVAMDKSFVDDLDVDSLSMVE
                     VVMACEDKFGVTIPDSEVKNLNTVGDAVNYIVANKS"
     gene            1940..3175
                     /locus_tag="AC16Y1_01030"
     CDS             1940..3175
                     /locus_tag="AC16Y1_01030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003954073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR5831079.1"
                     /translation="MSRNVVVTGLGATTPLGGNVASTWNALLSAQSGIGLLTDPRFSE
                     LPVRIAAVAKEDPETILEKHESRKMDRSQQLALIAAREAWADAGSPEIDPTRLGVAIA
                     TGVGGVISLLEQYDTLRERGASRVSPMTVPMLMPNGPAAVVGLEFGSQAGVHTPVSAC
                     ASGAEAIGYGAEMIRTGRADVVICGGTEGCIHPLTIAAFAAMRALSTQHGNAQSASKP
                     FDKDRDGFVMAEGAAIIVLESEEFAKARGAKIHAIYGGQGLAADAHHIAQPEPEGRGV
                     IAAMTRALADADLTPADIVHVNAHSTSTPAGDVAEAKAIRKVMGSHADHAAVTAVKGA
                     AGHLLGGAGSLAAIVAILSLKNRIVPQVTNLVNLDPEVDIDVVIGKPRELTQSGQLAA
                     LSNSFGFGGHDVVLAFKSL"
     gene            3175..4590
                     /locus_tag="AC16Y1_01035"
     CDS             3175..4590
                     /locus_tag="AC16Y1_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR5831080.1"
                     /translation="MSTPAAEDKKLMTPRRIGAVLDAGSVNYVAAQTEGIGAVSATGT
                     INGRDVVIFSNDPSFQGGSLGAAEGKVFTDAYEIAMKKQCPIIGIWHSGGARLQEGIS
                     SLKAFGEIFSAMTHASGKIPQISVVLGPAAGGGAYGPALTDIVILGPEGRIFVTGPDV
                     VRSVTGEDVDMLRLGGPEPHGRRSGVVHVVSDTEDDALAQTRYIADLLGNQGQLDVSK
                     ISDVDLGALLPDSPTRAYDVHPLVDSVLDSGTGVELHPRWAPNLVTALGRFGGRTVGV
                     VANNPLRLGGCLDATSAEKGARFVRMCDALGVPLLVLVDVPGYLPGVGQEWDGVVRRG
                     AKLLHAFAECVVPRITCVTRKSYGGAYIAMNSRSLGATRVMAWDGAEVAVMGPVAAIR
                     ILHRRRLAEVAPEDRESVENELAEEHKIIAGGLPRCIELGAIDEIVEPAKTRSALAKA
                     LAASSNKDGSFARGNHGNIPL"
     gene            complement(4587..5117)
                     /locus_tag="AC16Y1_01040"
     CDS             complement(4587..5117)
                     /locus_tag="AC16Y1_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3145 domain-containing protein"
                     /protein_id="HIR5831081.1"
                     /translation="MMGVPMTSYAMSSSTRGVVFIHSAPKGMCTHVEWSLGGLLGATA
                     TLDWTDQPIAPGMMRAELSWQGKPGLAAQIASILRAYPNLRFEVTEEPSLGFDGQRFV
                     STPALGLWSSQMSVIGEVLIPEAKLRTAMQDALSSGKSFVEVIDQVLGTAWDRELEPF
                     RYAGEGVPVRLLHQVS"
     gene            complement(5252..>5860)
                     /locus_tag="AC16Y1_01045"
     CDS             complement(5252..>5860)
                     /locus_tag="AC16Y1_01045"
                     /inference="COORDINATES: protein motif:HMM:NF023354.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent oxidative N-demethylase family
                     protein"
                     /protein_id="HIR5831082.1"
                     /translation="NLPIPLDPSVHPLEAASLLVQEDLVIMSPQNKQWVLTAASVCFP
                     SRWDLTTKVGADLHGIHEPVPHYEERIGYATDAMFDKLTPDRPVWRINWTVLDSPDLF
                     QPAQTGQKARQHHSQDLTEFGDNMFFRTERQTLWKLPKSGDILFTIRTYSDSINSLAS
                     RYPEFRANLGKTMATASIETRNYKGWGPIWEDMMAWANLSQI"
BASE COUNT         1529 a         1311 c         1467 g         1553 t
ORIGIN      
        1 atgctcgact tagattactt tcaaatcaag atggcactat cttgcatgat ggccgcgagg
       61 ttctaaaacg cttagttaca caagtaagca atccagttcg ctgggattta tgtatgaaaa
      121 ctatgttgga tttaggtgta acggcaatga tcgagttgcc gccggcagga acattaattg
      181 gcttagccaa acgttcaatg ccaggcgttg agttactcgc tctaaaaact ccggcagatc
      241 ttcctgccgc acttgacctg gtttccagac acagttctgt aagtccggtt ggcgatcgcc
      301 caacttggcg tttagttgta gcaccttata aaggaattat tgaatttaat gtttcagaag
      361 aacctggaac tgaattgaaa gacaacgaaa aggttgccac gatcagaact ttacgcgacg
      421 aatatgaagt taccgctcca cacggtggct caatcgttga atggttagta actaatggtg
      481 atccagttaa tccaggacag ccactacttc gtttacaccc aagagcgggc gcatgactaa
      541 attaaaaact ccagcgccga catcacatgc tggactaatc ggactcgggg tttatcgccc
      601 atcgagagtt gtgcctaatt ctgagattgt tgacgcaatc gattcaagtg atgaatggat
      661 tagagaacgt tctggaatta tcgaacgccg atttgctggt ccggcagaga ctgctgcatc
      721 gatgggagcg gcggcggcaa aaaatgcaat tgccgatgcg gggctgactg tagaacaaat
      781 cgatgtagtt ttggttgcca cattttccca ccgctatcaa actccatcag ccgcagttga
      841 agttgcggca gaaatcggtg cagtaaatgc aagtgcattt gacatctctg ctgcatgcgc
      901 cggattttgt tacggaattg gtttagctga tgctttgatc cgagccggaa atgcaaaaaa
      961 tattttggtt attggttcag aaaaattaag tgacattacc aacatgtcag atcgcggtac
     1021 ggcttttatt ttcgccgatg gtgctggtgc agtggtagtt gggccgacac aagaacaagg
     1081 tattggacca acaatctggg gtgcggatgg cactgcacgc gaagcaatca tcatggaacc
     1141 cgacctagtt actgccggta aacaaggagt gccgtcactt ttaactatgc aagggcaagc
     1201 agtatttcgc tgggccgtgg gaagtatggg cgatgtttgc cagcgcgctt tagacgctgc
     1261 agaaattgaa actagtgact taaaagcatt cgtgccacat caagcaaata ttcgaattac
     1321 tgatgcgatc gtaaaaaggt ttaactttcc agactcagta gtaattgcca aagatattca
     1381 aactgctggc aatacatctg ctgcttcaat cccactagca ctgcatgcat tgcgcgaagc
     1441 aggccaagtt aaaaaaggtg atccgttttt aatggttggc tttggcgcag gcctggcttt
     1501 tgcgagccaa gtagcgttag ttccttaagt tttaagttct tgaaagagaa cttaaaaaac
     1561 gacccgcaac cgcgggaagt cccaccgata aaggagagaa agtatgtcac aggaggacat
     1621 cctcgcgggc ttagccgcga tcgttaacga agtcgctggt attccagctg aaagcgttgc
     1681 aatggacaaa tcatttgtcg atgaccttga cgttgattca ttatcaatgg tcgaagttgt
     1741 tatggcatgt gaggataagt tcggcgtaac tattcctgac agtgaagtta agaatctaaa
     1801 tactgttggt gacgctgtta actacatcgt ggctaacaag tcataacaaa gtgatcttgt
     1861 aaaggtgggc ggcaaaagtg tcgcccacct acaaaagccc gcaaaactta ttcacccatt
     1921 aactgaaagg tcgacaacta tgagccgcaa tgttgtcgtg actggcttag gtgcaacaac
     1981 tccactcggt ggcaatgttg cttcaacctg gaatgcgtta ctttctgccc agtcaggcat
     2041 tggattatta actgatccaa gattttctga attgcctgtt cggatcgcag cagttgcaaa
     2101 agaagacccc gaaacaattt tggagaaaca cgaatccaga aaaatggatc gctcgcaaca
     2161 acttgcgcta attgctgccc gagaagcttg ggctgacgca ggtagtcccg aaattgaccc
     2221 aactcgactt ggcgttgcaa ttgctacagg tgttggtggc gttatttcac ttctggaaca
     2281 atacgacacc ttgcgcgaac gaggtgcttc tcgagtttca ccaatgactg ttccgatgtt
     2341 gatgcctaac ggaccagcgg ccgttgttgg tcttgagttt ggttcacaag caggagtaca
     2401 tactccagtt tcagcttgcg catcaggtgc agaggcaatt ggctacggtg cagaaatgat
     2461 taggactggt cgcgctgatg tagtcatttg tggtggcacc gaagggtgta ttcacccact
     2521 gacaattgct gcgttcgccg caatgcgcgc actttcaact caacatggca acgcgcaatc
     2581 ggcatcaaag ccattcgata aagatcgcga tggctttgtc atggctgaag gtgccgcaat
     2641 tatcgtttta gaatccgaag aatttgctaa agctcgtggt gcaaagattc atgcaatcta
     2701 tggtggtcaa ggtttagctg ccgatgctca ccacattgcc caaccagaac ccgaaggtcg
     2761 cggggttatt gcggcaatga ctcgtgcatt ggccgatgct gatttaactc cagctgacat
     2821 tgtgcacgtg aatgcgcact caacctcaac acctgcagga gatgtggcag aagcaaaagc
     2881 tattcgaaaa gttatgggta gccatgccga tcacgctgca gtcactgcag tcaaaggggc
     2941 agctggtcac ttacttggtg gtgccggttc gcttgcagct attgtggcga ttctttcgct
     3001 aaaaaatcga atagttccac aggttacaaa cttagtgaat ttagatccag aagttgatat
     3061 cgatgttgtt attggtaaac cgcgtgagtt aactcaatct ggccaactgg ctgcattaag
     3121 taattctttt ggtttcggtg gccacgatgt cgttctagct tttaagagtc tgtaatgagt
     3181 acaccagcag ctgaagataa aaaattaatg acaccccgca gaattggtgc ggtactagat
     3241 gcgggttcag ttaattacgt tgcggcgcaa accgagggca taggtgcagt aagcgccaca
     3301 ggcaccatca acgggcgcga tgtagttatt ttttcgaatg atccatcatt tcaaggtggt
     3361 tcgttaggtg ctgccgaagg caaagttttt actgatgcct acgaaatagc catgaaaaaa
     3421 caatgcccca ttattggaat ttggcattcg ggtggggcaa gactgcaaga gggtatctct
     3481 tcgttaaaag ctttcggtga aatctttagt gcgatgaccc acgcttcagg aaaaatccca
     3541 caaatttctg tagtacttgg cccagctgct ggtggtggcg cttatggtcc agcgcttaca
     3601 gacatagtga ttcttggacc ggaaggtcgc attttcgtaa cgggtccaga tgtagttcgt
     3661 tcagtaactg gtgaagacgt agacatgctt cgactaggcg gcccagaacc tcatggtcgc
     3721 cgaagtggcg tagtgcacgt ggtgagcgat acagaagacg atgctttagc tcagacacgt
     3781 tacatcgcag atctacttgg taaccagggt caattagatg tgagcaaaat ttctgatgtt
     3841 gacctaggcg cgctacttcc cgattctcca actcgggctt acgacgtgca tcccctagtt
     3901 gactcagtat tagattcagg aactggcgtc gaacttcatc ctcgttgggc gccaaatcta
     3961 gttactgctc ttggccgctt tggtggaaga accgttggcg tcgttgccaa taaccctttg
     4021 cgattgggcg gatgtttaga tgcaacttct gccgaaaaag gtgctcgctt cgttcgaatg
     4081 tgtgatgctc tgggtgttcc gcttttagtg ctagttgatg ttccgggtta cttacctggt
     4141 gttggtcaag agtgggatgg cgtggttcgt cgtggcgcta agttgttaca cgcttttgca
     4201 gaatgtgtgg tgccacggat tacttgcgtc acacgcaaat catatggcgg tgcttacatc
     4261 gccatgaact cacgtagttt gggtgcaact cgagttatgg cttgggatgg cgctgaagtt
     4321 gcagtgatgg gcccagttgc cgcaattcga atcctacata gacgcagatt ggctgaagta
     4381 gctccggaag atcgcgaaag tgtggaaaat gaattggctg aagagcacaa gattattgct
     4441 ggtgggctgc cacgttgtat tgaacttggg gcaatcgatg agattgttga acctgctaaa
     4501 acaaggagtg cattagctaa agcacttgcg gcaagtagta ataaagacgg aagttttgct
     4561 cgtggtaatc acggcaatat tcccctttaa ctaacttggt gtaacaacct aactggcaca
     4621 ccttcaccgg catacctaaa tggttctaat tcccggtccc aagctgtgcc aagtacttga
     4681 tcaatcactt cgacaaaaga ttttccgctg cttagtgcat cctgcatcgc ggtacgtaac
     4741 ttcgcttctg gaattaatac ttcgccaatc acactcattt gactactcca caggccaagc
     4801 gcgggagttg aaacgaagcg ttgtccatca aaacccaaac ttggttcctc ggtaacttca
     4861 aaccttaaat ttggatacgc ccgcaaaata gatgcaatct gagctgcaag ccctggtttg
     4921 ccttgccagg aaagttcggc acgcatcatt cctggtgcta ttggttgatc ggtccagtcg
     4981 agagttgcag ttgcgccaag taggccgcca agcgaccact caacatgagt gcacatacct
     5041 tttggagcgc tgtggataaa aactaccccg cgcgtcgaag atgacattgc ataactagtc
     5101 atcggtaccc ccatcatcag ctgcaatttg ccttccccag catttgcaat cgatgggatg
     5161 tcttaacaat gaggataacc cctgctaact aataattacc agttgaaaag accgaaaacc
     5221 cttaattatt agtaataggt aatggatttc attagatctg cgacagatta gcccaagcca
     5281 tcatgtcttc ccagatcggg ccccaacctt tgtaattacg cgtctcgata ctcgccgttg
     5341 ccatggtttt gccaaggttg gctctaaatt cgggataacg acttgccaga ctgttaattg
     5401 aatcgctgta ggttctgatc gtaaacaaga tgtcgccgga ctttggtaat ttccaaagtg
     5461 tttggcgctc agttctaaaa aacatgttgt caccaaattc agttaaatcc tgtgaatgat
     5521 gttgccgagc tttttggcca gtttgagctg gttgaaaaag gtctgggcta tctaataccg
     5581 tccaattaat gcgccagacc ggacgatctg gcgttaattt gtcgaacatt gcatccgttg
     5641 cataaccgat ccgttcttcg tagtgcggaa cgggctcatg aatgccgtgc agatcggcac
     5701 caacttttgt ggtcaaatcc cagcgacttg gaaaacaaac gctggcagcc gtaagcaccc
     5761 attgtttatt ttgtgggctc ataattacta ggtcttcttg tactaataga ctcgcggctt
     5821 caagtggatg cactgaggga tccagcggaa ttggcaagtt
//
LOCUS       DBNHVN010000057         1161 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1495855, whole genome shotgun sequence.
ACCESSION   DBNHVN010000057 DBNHVN010000000
VERSION     DBNHVN010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1495855"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /gene="dcd"
                     /locus_tag="AC16Y1_01050"
     CDS             complement(<1..478)
                     /gene="dcd"
                     /locus_tag="AC16Y1_01050"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017948066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HIR5831083.1"
                     /translation="MLLSDKDIKLEIGAARIGIEPYDVAMVQPSSIDVRLDRFFRVFE
                     NHRYPHIDPSTEQAELTREIAPVGDEPFVLHPGEFVLGSTYEVVTLPNDIAARLEGKS
                     SLGRLGLLTHSTAGFIDPGFSGHVTLELANVATLPILLYPGMKIGQVCFFRMTSEAE"
     gene            486..1103
                     /locus_tag="AC16Y1_01055"
     CDS             486..1103
                     /locus_tag="AC16Y1_01055"
                     /inference="COORDINATES: protein motif:HMM:NF016149.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0046688 - response to copper ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HIR5831084.1"
                     /translation="MLPEQASGMGSGLGIWLDYYGVMRFISKISTVVLAVISSLLLVA
                     SAWAHTDVVSTSPIDGAVIDVTPAEISITFSEPPIEAGAAILLADVSGTQLPLGEVNF
                     EGAKISAAAPIDLPAGDYVVTWRVSAEDGHALTGEFSFTFTGKVSVNDPNNTITAGSE
                     ETAVAIPVMDDEVTGNSGGTLWISLLAVLIASAIVAAIVASRKRQ"
BASE COUNT          343 a          240 c          255 g          323 t
ORIGIN      
        1 gttctgcctc agaagtcatt ctgaagaaac aaacctgacc aattttcata cctgggtaaa
       61 gcaagatagg caatgtggca acgtttgcta actccaaagt tacgtgcccc gaaaaaccag
      121 gatcgataaa acctgcagtt gagtgcgtta aaagtccaag acggcccaaa ctagatttac
      181 cttcaagtcg agcggcaatg tcatttggca aagttacgac ttcgtaagta ctacctaaaa
      241 caaactcacc tggatgcaaa acaaacggtt cgtcacctac tggagcaatt tcgcgagtta
      301 attcagcctg ctcagttgac ggatcaatgt gtgggtaacg atgattttca aaaactcgaa
      361 aaaatcgatc taagcgaaca tcaatacttg atggttgaac cattgctacg tcataaggtt
      421 caatgccaat tcgggcagcg ccaatttcta atttgatgtc tttatctgaa agcaacacag
      481 gcaaaatgtt acccgagcaa gcatctggaa tgggttcggg actaggaatt tggcttgatt
      541 actatggagt tatgagattt atttcaaaaa tatctacagt tgttcttgca gtaattagct
      601 ccttactttt agtggcttca gcctgggcac ataccgatgt agttagcact tctccgatag
      661 atggcgccgt aatcgacgtg actccagcag aaatctcaat cactttttct gagccaccaa
      721 ttgaagcagg cgcagcaatt ctgttagcag atgtttccgg aacgcagctt ccactcggcg
      781 aagtaaattt cgaaggcgca aaaattagtg cagcagcccc aattgattta ccagctggtg
      841 actacgtggt gacttggcga gtctctgccg aagatggtca cgcattaact ggtgaattta
      901 gttttacttt tactggaaaa gtttcggtaa atgatccaaa taatacgatt accgctggat
      961 ccgaagaaac tgcagttgca attccggtta tggacgatga agttactgga aattcaggtg
     1021 gaacgctttg gataagcttg cttgcagttt taattgctag cgcaatagtt gcagcgatag
     1081 tcgcttctcg gaaaagacag taattgaata aaactttaac cagattagtt attcccactt
     1141 ttgcgatagt tggtttagtt t
//
LOCUS       DBNHVN010000058         4843 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_827391, whole genome shotgun sequence.
ACCESSION   DBNHVN010000058 DBNHVN010000000
VERSION     DBNHVN010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_827391"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /gene="rpsO"
                     /locus_tag="AC16Y1_01060"
     CDS             complement(<1..269)
                     /gene="rpsO"
                     /locus_tag="AC16Y1_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR5831085.1"
                     /translation="MSLDAKIKQEIITKYGANASDTGSPEVQIALLTQRINDLTGHLK
                     SHKHDHHSRRGLLLLVGQRRRLLDYLTKTDINRYRSIIEKLGIRR"
     gene            complement(368..1288)
                     /locus_tag="AC16Y1_01065"
     CDS             complement(368..1288)
                     /locus_tag="AC16Y1_01065"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR5831086.1"
                     /translation="MNSKQSVVVTVGVFDGVHAGHRYLISTAKKIAQQSDSLLVAASF
                     DPHPVSFLRPDKFLGLITLPEFRATLLKQAGAQRVEFINFDQKVAHMSPDEFIETIVL
                     DELNASTIVVGENFRFGANASGDVTTLTTLANKFGFELEVVKLIGDQQTWSSTRVRNN
                     LLTGDVKAAREILGRPHRLTGKVVHGDHRGRELGYPTANLEVFGNLIIPADGVYSALV
                     YTNSEVLPAAVSIGTNPTFDDVVSRRVEAYVLDRTDLDLYGQEISVDFLDFVRPMDKF
                     SDVESLLAQMALDVENTRLQISDFLDSPSH"
     gene            complement(1291..2157)
                     /gene="truB"
                     /locus_tag="AC16Y1_01070"
     CDS             complement(1291..2157)
                     /gene="truB"
                     /locus_tag="AC16Y1_01070"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR5831087.1"
                     /translation="MATDGIVLVDKPEGPTSHDVVAKARRIFGTRKVGHAGTLDPMAT
                     GMLILGIGKATRLLGYLTATDKEYLATIRLGQATNTDDAQGEVTLSAATAGISDEDII
                     NAIRELRGPISQVPSSVSAIKVNGKRAYALVRKGEQVDLPARDVMIHDFAVLEINRIE
                     TFIDVKVRVSCSAGTYVRALARDLGNKLQVGGHLTALRRTKSGKFTQMRPLTDIENSP
                     SVIELAEAVKLLFPVVEISGDDIKKAKNGMRVPLPVEVPPSVIGVFTNTGEIISLCEN
                     VAGELVPVVVFN"
     gene            complement(2158..3150)
                     /locus_tag="AC16Y1_01075"
     CDS             complement(2158..3150)
                     /locus_tag="AC16Y1_01075"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013528.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR5831088.1"
                     /translation="MSNVTQTLDWNGAVSAVRLAKSVLLIAHVTPDADALGSALALGL
                     ACESLGKDVQVSVGESGFTMPQSLAFLPRTDLVVSPEALKPADLVIVCDTASVARLGV
                     LADVVAETQTSIVLDHHPSFNGFGTILLVDPTAAATAAIAMELIERLDVELTKDIASA
                     LYSGLATDTGSFKFQATTAQTLRIAAKLFDAGIDHAQLARQLFDDEPFEALIMLGESL
                     QRAELDKTAANGLGLAYTSIAINQRHGLTELAMERVIEAIRRATEAEVAAVFKQADDK
                     HWKVSLRSKTTVNVGELATQLGGGGHHFAAGYTSGTDLSESIAQLKSQLNLLGD"
     gene            complement(3153..3584)
                     /gene="rbfA"
                     /locus_tag="AC16Y1_01080"
     CDS             complement(3153..3584)
                     /gene="rbfA"
                     /locus_tag="AC16Y1_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR5831089.1"
                     /translation="MVDLARARRLGERIQEIVAETLELKIKDPRLGFVTVTNVKVSPD
                     LHDATVFYTILGTEEEATKSAQALESCKGLVRTEVGKRTGVKFTPSITFVRDALPDSV
                     RLIDDLLHEAASADAKVAEQAAKANFAGEADAYRTSKDKEA"
     gene            complement(3627..>4843)
                     /gene="infB"
                     /locus_tag="AC16Y1_01085"
     CDS             complement(3627..>4843)
                     /gene="infB"
                     /locus_tag="AC16Y1_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR5831090.1"
                     /translation="EVPIVVAVNKIDKEGADPTKVRGQLTEYGLVPEEYGGETIFVDV
                     SAKNNLNIDELVESILLTADAGLDLRANPTQDAQGVAIEAHLDRGRGPVATVLVQRGT
                     LRPGESIVIGDAFGRVRAMLDENGETVEFAGPARAVQVLGLTSVPDAGDTFLVVDEDR
                     TARQIAQSRQARERNAQLAARRGRHTLEDVLKTLEKGEVAELKIIIKGDVSGSVEALE
                     DALLNIDIGDENVLLRVIHRGVGAITENDVSLAAASQALIIGFNVRPEGKSRDLADRL
                     DVEIRFYNVIYSVIDDIESSLKGMRKAEYEEAELGSAEVREVFRSSKFGNIAGCIVTT
                     GEIKRKAKARLVRDGAVVAADLSIDTLRRFKDDATEVREGFECGIGLGSFNDIKVGDI
                     IETYEMREKVLS"
BASE COUNT         1306 a         1187 c          996 g         1354 t
ORIGIN      
        1 tagcgacgaa tacctagttt ttcgataatt gaacggtaac gattgatgtc ggtcttagtt
       61 aggtaatcaa gtaaccgacg acgttgaccg actagcagaa gcaaaccgcg acggctgtgg
      121 tgatcgtgct tgtgtgactt taagtgccca gttaagtcat tgatccgttg agttagaagt
      181 gcaatctgaa cttctggact tccggtatca gatgcatttg caccgtactt cgtaattatt
      241 tcttgtttga ttttcgcgtc tagcgacatt attttccttc gggcgcggtc agggattgtt
      301 gccaatccaa tgtttagttg tcacgtattt cagagcgccc gctccaaaac cgtctggtta
      361 aaaggtatca gtgacttggt gaatctagga aatcgctaat ttgaagtcta gtattttcaa
      421 cgtctagagc catttgcgcc aagagcgatt ccacatctga aaacttatcc atgggtctaa
      481 caaaatctag aaaatcaacc gagatttctt gaccgtacaa atctaaatcg gttcgatcca
      541 atacatatgc ctccactctg cggctaacca cgtcgtcaaa tgttgggtta gtcccaatac
      601 tcactgcggc cgggaggact tctgaattcg tgtaaaccaa agctgagtac accccatcag
      661 caggaattat taagtttcca aaaacttcaa gatttgcagt tggataaccg agttcacgac
      721 ctctatggtc tccgtgcacg acttttccag ttaagcggtg cgggcgaccc aaaatttctc
      781 gtgcagcttt aacgtctcca gttaaaagat tatttcgcac tcttgttgat gaccaagtct
      841 gttgatcccc gatcagtttg acgacttcta attcaaagcc aaacttattc gccagggttg
      901 taagagtagt tacatcccca gaggcattcg caccaaatct gaaattttca ccaacgacaa
      961 tggtgcttgc atttagctca tcaagcacaa ttgtctcaat gaattcatca ggtgacatat
     1021 gtgcaacttt ttgatcaaaa ttaatgaact ctacgcgttg tgctccagct tgcttaagaa
     1081 gcgtagctcg gaactctggc agggtaatta atcctaaaaa cttatctggt cgcaaaaaac
     1141 ttactggatg tggatcaaat gatgctgcaa ctaataacga atcactttgt tgggcgattt
     1201 ttttggcagt tgaaattaga tacctatgcc cagcatgaac tccatcgaaa actccgacgg
     1261 taacaacaac tgattgcttg gaattcatct ctagttaaac accactaccg gaactaattc
     1321 ccccgccacg ttttcacaca atgaaattat ttcaccagta ttagtaaaca caccaattac
     1381 agatggtgga acttcaacag gtaagggaac tcgcattcca ttttttgctt ttttgatatc
     1441 gtcgccagaa atttcgacta ctgggaaaag cagttttacc gcttcagcta attcgataac
     1501 agaaggtgaa ttctcaatgt cagtgagcgg tcgcatctgc gtaaatttgc cagacttagt
     1561 tcggcgtagc gcggttaaat gcccaccaac ttgtaattta tttcccaaat cgcgagctaa
     1621 cgctcgtaca taagttccgg cactacaact aactcttact tttacatcaa taaaagtttc
     1681 aatgcgattt atttccagaa ctgcaaaatc atgaatcata acatctcgtg ctggaagatc
     1741 tacctgctcg ccttttcgta ccaacgcata ggcacgcttt ccattaactt ttattgcgct
     1801 aactgaactg ggaacttgag aaatgggtcc gcgtaattct ctaattgcat taataatgtc
     1861 ttcatcacta attccagcag tggcagcgct tagggtcact tcaccttgtg cgtcatctgt
     1921 attggtagct tgccctaatc gaatagtcgc caaatattct ttatctgttg cagttaaata
     1981 gccaagtaag cgagtggctt tgccgattcc aagaatcagc atcccggtag ccatcggatc
     2041 cagtgttccg gcatgcccga cttttcgggt tccaaatatc cgacgggctt ttgctaccac
     2101 gtcgtgcgaa gttggtcctt ctggcttatc aaccagaacg attccgtcgg ttgccaatta
     2161 gtcacccagt aaattcaatt gtgatttaag ttgcgcaata ctttcggaca agtctgttcc
     2221 acttgtataa ccagcagcaa agtgatgtcc accaccgcct aattgtgttg ccaattcgcc
     2281 aacatttact gttgttttac tccgcagcga aactttccag tgtttgtcgt ctgcttgttt
     2341 aaatacggcg gcgacttctg cttcagtggc acgtcggatt gcttcaataa ctcgctccat
     2401 tgccagttcg gttaaaccgt ggcgctggtt aattgcaata gaggtataag ccagccctaa
     2461 cccgttagcc gcagtcttat ctagctcagc gcgttgtagc gattcaccta acataatcaa
     2521 cgcctcaaat ggctcgtcat caaaaagctg tctagctaac tgcgcatgat ctattccggc
     2581 atcaaatagc tttgctgcaa ttcgcagtgt ttgcgcagtg gtggcttgaa acttaaacga
     2641 acccgtatcg gtagccaatc cagaataaag agcactagcg atgtctttag ttaactcgac
     2701 atctaatcgt tcaattaatt ccatcgcgat ggccgcagtt gctgcggccg ttgggtcaac
     2761 caaaagtatg gtgccaaaac cgttaaaaga aggatgatga tcaagcacga ttgacgtttg
     2821 agtttccgct actacgtcag cgagcacacc aagtcgagca actgatgcgg tatcacaaac
     2881 aattactaag tcggcaggtt tgagtgcttc gggtgaaaca actaaatcgg ttctaggtaa
     2941 gaatgctaaa gattgcggca tggtaaaacc agactcacca actgaaacct gcacatcttt
     3001 accaagcgac tcacatgcca aaccaagagc caatgcacta cccaacgcat ccgcatcagg
     3061 tgtgacatga gcaatgagca acacagactt tgctaaccga actgcgctta cggcaccatt
     3121 ccagtcgaga gtctgggtta cgttactcat aattaggctt ctttatcttt acttgttcga
     3181 taggcatcgg cctcgccagc aaaattcgct ttagcggcct gttcagctac ttttgcgtca
     3241 gcacttgcag cttcgtgtaa taagtcatct attaaacgaa ctgaatctgg cagtgcatcg
     3301 cgaacgaagg taatacttgg cgtaaatttc acgccggtgc gcttgccaac ttcagtgcgg
     3361 accaaacctt tacatgattc caaagcttga gcagatttag tcgcctcttc ctcggtacca
     3421 agaattgtgt aaaagactgt tgcgtcatgt aagtcgggtg aaactttcac gttagttaca
     3481 gttacgaatc ccaggcgtgg atctttgatt tttaactcga gagtctcagc aacaatttct
     3541 tgaatacgtt caccgagccg tcgagcccga gccaaatcaa ccatggcaat ctcctatcta
     3601 aaaaatgtta aaaagttcag tgttgactag ctaagaactt tttcacgcat ttcataggtt
     3661 tcaatgatgt cgccaacttt aatgtcattg aacgaaccga gtccgatacc gcactcgaat
     3721 ccttccctaa cttcggttgc gtcatctttg aagcgtctta acgtatcgat cgacaagtca
     3781 gcagctacaa ctgcaccgtc acgcaccaag cgagctttag ctttacgttt gatttcgccg
     3841 gtagttacta tgcaacctgc gatgtttccg aacttgctag aacggaatac ttcgcgaact
     3901 tcggcactac ccaattccgc ttcttcgtat tcagccttac gcataccctt aagcgacgat
     3961 tcgatgtcat caattactga atagataacg ttgtagaacc gaatctctac gtctaaccga
     4021 tcagctaagt cacgggactt accttcaggt cgaacgttga atccaataat caatgcttgc
     4081 gaagctgcag ccagtgacac gtcattttca gtaatcgcac ctacaccgcg gtggataacc
     4141 cgaagtaata cattttcatc gccaatgtcg atgtttagta aggcatcctc gagcgcttca
     4201 accgaaccag atacgtcacc cttgataatt attttgagtt cagcgacttc acccttttca
     4261 agagtcttaa gtacatcttc aagtgtgtga cgtccacgac gtgcagcaag ttgagcattg
     4321 cgctcacgag cctgacgtga ctgcgcaatt tgtcgggcag tgcgatcttc gtcaacaacc
     4381 aagaaggtgt caccggcatc tggcactgag gttagaccaa gtacttgaac tgctctagca
     4441 ggacctgcaa actcaacggt ctcgccattc tcatcaagca ttgctcgaac acgaccaaac
     4501 gcatcaccaa taacgattga ttcacctggt cgcagagttc cgcgctggac tagcactgtt
     4561 gcaactgggc cacgacctct atcgaggtga gcttcaattg ccacaccctg cgcatcttgt
     4621 gttggattag cgcgtagatc caggccagca tcagcagtaa gcaaaataga ttcaactagc
     4681 tcatcaatgt ttagattatt ttttgctgaa acatccacaa agattgtttc gccaccatac
     4741 tcttcgggta ccaaaccgta ttcagtaagt tgaccacgaa ctttagttgg gtcagcgcct
     4801 tctttgtcga tcttgttaac agcaacaact attggcactt ctg
//
LOCUS       DBNHVN010000059         2655 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1200187, whole genome shotgun sequence.
ACCESSION   DBNHVN010000059 DBNHVN010000000
VERSION     DBNHVN010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1200187"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="AC16Y1_01090"
     CDS             complement(<1..753)
                     /locus_tag="AC16Y1_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007823757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HIR5831091.1"
                     /translation="MGIKQIFRSPMFWIALAIFALLFGSNSFNSAGAAKEVQTWKIIE
                     DIQQGRAKSVTLVDGDQLIEVTLTDDTKESAEFITGQAPSLISLLQSQKNAGNLPDGY
                     DIVVPRQSFLGTLFMSIFPIILILAIFFFLMSQMQGGGRIMSFGKSRAKLITKDMPQT
                     TFADVAGADEAKEELVEIKEFLQEPAKFQALGAKIPKGVLLYGPPGTGKTLLARAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQA"
     gene            916..1209
                     /locus_tag="AC16Y1_01095"
     CDS             916..1209
                     /locus_tag="AC16Y1_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014847804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HIR5831092.1"
                     /translation="MIFKQVLGERPYQIPDISSETWASLAPRQIRLADLSTIKSTLDL
                     NELLASDSTFFGDLFAHVVRWNGNLYLEDGLHRAVRAALQGRTSLHARVYEIN"
     gene            complement(1206..1949)
                     /locus_tag="AC16Y1_01100"
     CDS             complement(1206..1949)
                     /locus_tag="AC16Y1_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR5831093.1"
                     /translation="MNVLEVTNLRKCFGNEVVLNDLNLVVPEHTATVLIGASGSGKST
                     LLRCINLLEQIDDGEIWIDGIEISDPEVDEDAVRRQLGMVFQSFNLFPHMSVLENITL
                     APIKVHGISKEQAVTEAKQLLERFDLLDKADQYPDRLSGGQQQRVAIIRSIANKPRLL
                     LLDEVTSALDPVLVNEVLSIVRDLKQDGMTMVLATHEMGFATQVADEVCFLKDGFILE
                     RGTPNQVLENPQLPETKDFLKRVHEAGRL"
     gene            complement(1946..>2655)
                     /locus_tag="AC16Y1_01105"
     CDS             complement(1946..>2655)
                     /locus_tag="AC16Y1_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR5831094.1"
                     /translation="WPVVKKTFFDWEYGQTVISTVIRGLITNITLTVVASIAIGIIAL
                     ALALARTSRSAALTPFRLLATAYVDVFRGIPLLLVILLIGFGVPALRLTGVTNSVIIL
                     GTTAVVITYSAYVAEVIRSGILSIHPSQRAAARSLGLSQMQTLRYVVLPQAFKRVVPP
                     LLNDLVALLKDTGLISILGVTDAIRAGQIAASRTFNYTPYVIAAILFLLITIPLTRYT
                     DRVLKRSIEKQNAQGNA"
BASE COUNT          815 a          586 c          533 g          721 t
ORIGIN      
        1 tgcttgctcg aaaagatcac gtacgcgact ggcacctaca ccaacaaaca tttccacgaa
       61 atctgaacca gaaattgaga agaacggaac tccagcttcg ccagctaccg cacgggcaag
      121 tagtgtctta ccagtccccg gcggaccata aagtaaaacg ccttttggaa tctttgcacc
      181 aagggcttga aatttagcag gttcttgtaa aaattcttta atctcaacaa gttcttcttt
      241 agcttcatct gctccggcaa catctgcaaa tgttgtttgt ggcatgtctt tagtaattaa
      301 tttggctcgt gatttaccaa atgacatgat gcggccgccg ccttgcatct ggctcattag
      361 gaagaagaaa atcgccaaaa tcaaaataat tgggaagatc gacataaata aggtgcctaa
      421 gaatgattgg cgtggcacca caatgtcgta accatcaggc aagttgccgg cattcttttg
      481 cgactgaagc aaagaaatta gtgaaggtgc ctggccagta ataaactcag cagattcctt
      541 agtgtcatct gtgagagtca cttcgatcaa ttgatcaccg tcaaccaaag ttacagactt
      601 ggcgcggccc tgctggatgt cttcaataat tttccaggtc tggacttctt tagccgcacc
      661 agcactattg aaagagttag agccgaataa caacgcaaag attgcaagcg caatccaaaa
      721 cataggtgaa cgaaaaatct gtttaatgcc catgtttaag gtaaaactcc cctatttatt
      781 ggttgaatta ttaaggtact accccaaacc gatatgtgcg aaatggttct caaccggcga
      841 aacctatgac acgcgacaag ttaagaaccc cattaaatac agggttttca ttcatcgctt
      901 ccatagtgtt gtgctgtgat atttaagcaa gtcttgggtg agcggccata tcaaattcca
      961 gatatttcta gcgaaacttg ggcttcatta gcaccccgcc aaattcgatt agctgactta
     1021 tcaacgataa aaagcacgct tgatctaaat gaattactgg ctagcgactc aacttttttt
     1081 ggtgacttat ttgcccacgt agtgcgatgg aacgggaatc tctatttaga agatggttta
     1141 catcgagcag ttcgtgctgc actacaaggt cgcacctcgt tacacgcccg cgtttacgaa
     1201 atcaactaaa gccgaccggc ttcatgcacc cgttttaaga aatctttagt ttctggtaac
     1261 tgtggatttt ctaaaacttg gtttggcgtt cctcgttcca aaataaaccc atcttttaag
     1321 aaacaaactt cgtcagccac ctgagttgca aatcccattt catgtgtggc caaaaccata
     1381 gtcataccat cttgttttaa gtcacgcaca attgataaaa cttcattcac taaaactgga
     1441 tcaagcgcag aagtcacttc atctaaaagc aagagtcgtg gtttattcgc aattgaacga
     1501 ataattgcaa cgcgttgttg ttggccacca cttaatcgat ctggatattg atcggcttta
     1561 tctaagagat caaatctttc caggagttgt ttagcttctg taacagcttg ttccttacta
     1621 ataccatgga cttttattgg cgcaagcgtg atgttttcta aaacgctcat gtgtggaaac
     1681 aagttgaatg attgaaagac catacccaat tggcgacgaa cagcatcttc atcaacttcg
     1741 ggatctgaga tttcaatccc atcaatccaa atctcgccat cgtcaatctg ttccagcaag
     1801 ttaatacagc gcaacaaagt agattttcct gagcctgatg cgccaattaa tactgtggcg
     1861 gtatgttctg gaactactaa attcaaatca tttaaaacaa cttcatttcc aaaacattta
     1921 cgcaagttgg taacttcaag aacgttcatg cgttaccttg cgcattctgc ttttcaatcg
     1981 aacgcttaag tactcgatcc gtatatctgg taagtggaat tgtgattaac aagaaaagaa
     2041 ttgcggctat tacataaggt gtgtaattaa aagtgcgtga tgcagcaatc tggcctgctc
     2101 gaattgcatc tgtcactccc agaatcgaaa ttaaaccagt gtctttcagt agtgcgacta
     2161 aatcatttaa tagtggcggc acaactcgct taaacgcctg aggtaaaaca acataacgaa
     2221 gtgtttgcat ttggcttaaa cccaaagatc tagcagctgc gcgctggctt gggtgaatcg
     2281 acaaaatacc actgcgaata acttcggcaa cataagcgga ataagttatg acgactgcag
     2341 ttgtacccaa aattattacg ctattcgtaa caccagttaa gcgaagtgca ggaacaccaa
     2401 aaccaattag caaaataact aaaagcagag ggataccacg gaatacatca acgtaggcag
     2461 tagcaagtag ccgaaatggt gttaaagcag cagacctaga tgtgcgagct aaggccaagg
     2521 ctaaagcgat aatgccaatt gcgattgaag caaccactgt gagagtgata tttgtgatta
     2581 agcctcgaat aacggtgctt attactgttt gaccatattc ccaatcaaaa aaagtcttct
     2641 taacaactgg ccagc
//
LOCUS       DBNHVN010000060         4268 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_456872, whole genome shotgun sequence.
ACCESSION   DBNHVN010000060 DBNHVN010000000
VERSION     DBNHVN010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_456872"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="AC16Y1_01110"
     CDS             complement(<1..387)
                     /locus_tag="AC16Y1_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR5831095.1"
                     /translation="MNIQGLIDRFSREISKFGVVGLIAYIIDLSVFNFLRFADGQGIL
                     YDKPLTAKASSVIIATTFAYFGNRHWTFNKRSRRTFRREYTLFIGINAVGMVIALSCL
                     FVSHYVLGFQSALADNISANGVGLVLG"
     gene            574..1251
                     /gene="mtrA"
                     /locus_tag="AC16Y1_01115"
     CDS             574..1251
                     /gene="mtrA"
                     /locus_tag="AC16Y1_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019435185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system response regulator MtrA"
                     /protein_id="HIR5831096.1"
                     /translation="MVARILVVDDDQAISEILGIVLRAEGFEPYFCADGDKAMDVFHK
                     ANPDLVLLDLMLPGRDGIDICRAIRQESGVPIVMLTARSDSQDIVTGLESGADDYIVK
                     PIKNKELIARIRARLRKVETPTVKEIKVGDLVIDVAGHVVRRGNEILPLTVLEFKLLM
                     TLAKRPWQVFTREVLLQEVWEYQRSADTRLVNVHVQRLRAKVEKDPERPEIVLTVRGV
                     GYKAGTV"
     gene            1255..2808
                     /gene="mtrB"
                     /locus_tag="AC16Y1_01120"
     CDS             1255..2808
                     /gene="mtrB"
                     /locus_tag="AC16Y1_01120"
                     /inference="COORDINATES: protein motif:HMM:NF040691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system histidine kinase MtrB"
                     /protein_id="HIR5831097.1"
                     /translation="MSEIKLRSSFPKLWRRNLRLRLVTVTIVLSSIVLGLLGALLLQR
                     VTSGLLTSKESNVLTEATATAQEVQRLLNASDTGISTPNVTRLVDSAITAIAVRAGAT
                     GSYDALLLASPDISGQPERGTRLVSETSVPIELRKAVRESGKQAWTYSLLSYEDGRTE
                     KGIVVGSSVTIPRVGLYELYLLFPLTAEQQTLSLVRGVVGATGLAVLAGLGVLAWFVT
                     SRVTGPIRKAAQVAGELANGDLDRRLTVRGEDDLARLATSFNDMAESLKSQINRLESL
                     STLQQQFVSDVSHELRTPLTTVRMASEMIFEARNQLDPQVARSAELLRNQVDRFDLLL
                     SDLLEMSKIDAGATILDISTFDLSDLVSQEIESANEIAKKYETSITFVDQGGNSVLEG
                     DSRRVSRIVRNLLANAIEHAEQKPIEVKVKANESAVSVGVRDYGQGMNETDLERVFDR
                     FWRADPARQRTLGGTGLGLSISNEDAKLHQGELRVWAKPNQGAHFVLTLPLRNGEPID
                     ILPIKSGEK"
     gene            2805..>4268
                     /locus_tag="AC16Y1_01125"
     CDS             2805..>4268
                     /locus_tag="AC16Y1_01125"
                     /inference="COORDINATES: protein motif:HMM:NF052827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HIR5831098.1"
                     /translation="MKKLKFVFIAFLLTGCAGIPTSGQIKFGPEINSNQADQFIQVIG
                     RPPAPGMTQEQIAQGFLAALADSRDDFAIAKEYLTDQTARTWKSSAGITIYDPNNFQL
                     SVDDAIANISAAKLGEIDSTGYLTVASASATVSSQFRLVQDLQGEWRISELSDGVLLT
                     VNDIDRSFQGYPIYFLGPDQKTLVADTVLLPQTLTGSATALVQALLEGPSKKLDSAVL
                     SSFPTGTALTYGSVPVTEGVANIDLTNQILSADQQTRSLMSAQLAWTLSTLPNVSAIS
                     ITVSGQPISIAGVTSVQTVRDWAQFNPAQFTGNEILHYVRDNQVFSLNLNGEETLVVQ
                     VNPASRVNISKAFGAPQGGSIAAVSTDSKRVLLSTGRGGQFEQVATGEAISKPSWDKS
                     GGIFYSDYGVGIFEINSNREVRPVTFESNALASVSQVKQIAVAKDGIRVAMVVSDGSI
                     DLLIGGAIFKSTEATVISGLHLIERNINAIKDLTWQTP"
BASE COUNT         1217 a          835 c         1014 g         1202 t
ORIGIN      
        1 tccgagtact aaaccaactc cgttggcgct gatgttatcg gcaagcgcac tttgaaagcc
       61 aaggacatag tgacttacga agagacagga aagtgcaata accattccca cagcatttat
      121 gccaataaaa agcgtgtact cacgtcggaa agttcggcgt gatcttttgt tgaatgtcca
      181 atgacggtta ccaaaataag caaatgtagt cgcgatgata acgcttgatg cttttgcagt
      241 aagcggcttg tcatacagaa tgccttggcc atcggcaaat ctaagaaagt taaataccga
      301 taaatcgata atgtaggcaa tcaatccaac aacaccgaat ttgctaattt cacggctgaa
      361 ccggtcgatt aaaccctgga tattcaccca ctaatcttaa ctgcctctag atctattcaa
      421 gtgcaatcta gtcaggtgca atctagtcag gttgaatcta ttctggtgga tgcctattta
      481 ttttcatgac tgtttatatt gagcagattt ggcgggaata ccctttacgt ccgtttcgag
      541 gtaaatttct cgtattaatc gaaaggcgct tcggtggtag ctcgaatttt ggttgtcgat
      601 gatgatcaag caatttcgga gattctaggc attgtgttgc gcgctgaagg tttcgaacca
      661 tatttttgtg ccgatggcga taaagccatg gatgtatttc ataaagcaaa tcctgattta
      721 gttttactcg acttgatgct gcctggccgt gatggaattg atatttgtag agcaattcgg
      781 caggaaagtg gagttccgat cgttatgctc accgcacgaa gtgattcgca agatattgtt
      841 actgggcttg aatctggcgc cgacgattac attgtgaaac cgattaagaa taaagaactt
      901 attgcccgaa ttcgggctag actgcgcaag gttgagaccc ctacagtaaa agaaattaaa
      961 gttggtgact tagttattga cgtagctggt cacgtagtgc gacgcggtaa tgaaatttta
     1021 ccacttaccg tacttgaatt taagttattg atgactttgg ctaagcggcc atggcaagta
     1081 tttacgcgcg aagtactgct tcaagaagtt tgggagtacc aacgttctgc agatacacgt
     1141 ttggttaacg tccatgtgca aagacttaga gcaaaggtag aaaaagatcc agagcgacca
     1201 gaaattgtat tgactgttcg cggtgttgga tacaaagcag gaactgttta gttcgtgagc
     1261 gaaattaagt tacgctcttc gttcccaaaa ctttggcgac ggaatttacg tttacgccta
     1321 gtgactgtga cgatagttct ctcgtcgata gttttagggc tcttgggtgc attgttgttg
     1381 cagcgagtga catctggttt gctgacctca aaagagtcaa atgttttgac cgaagctaca
     1441 gctacagctc aagaagttca acgtttatta aatgcatctg atactggaat cagcacaccg
     1501 aatgtaaccc gattagtgga ctctgctatt actgctatcg cagtcagagc aggtgcaact
     1561 ggatcgtatg acgctttact acttgctagc ccggatatat cgggacaacc ggaacgtggt
     1621 actcgcttag taagtgagac atccgtgcca attgaattac gcaaggctgt tcgtgaatca
     1681 ggtaagcaag catggactta tagtctgctt tcgtatgaag atggtcgcac tgaaaaagga
     1741 attgttgttg gatcgagtgt gacgattcca agagttggtc tctacgagct ttacttactc
     1801 ttcccgctga ctgccgaaca gcaaacatta agcctggttc gcggtgtagt tggagcgaca
     1861 gggctagcgg tacttgcagg tctgggcgtc ctagcttggt ttgtgactag tcgcgttact
     1921 ggcccaatcc gaaaagccgc acaggttgca ggtgaattag ccaacggaga tttagatcga
     1981 cgattaactg tccgcggcga agatgattta gctaggttgg caacatcttt taatgacatg
     2041 gcagaaagtt taaaaagcca aatcaatcgc cttgaaagtc tgtcaacgct gcagcaacaa
     2101 tttgtttcag atgtatctca tgaattacgt acgccactta ctacagttcg tatggcgagc
     2161 gagatgattt ttgaggcgcg caatcagtta gacccacagg ttgccaggtc cgctgagtta
     2221 ttacgtaatc aagttgaccg atttgatttg ttgctcagtg acttacttga gatgagcaaa
     2281 attgatgcgg gtgcaacaat tttggatatc tctacgtttg atctaagcga cctagtctct
     2341 caagaaattg aatccgcaaa tgaaattgcc aaaaagtacg aaactagtat cacctttgtt
     2401 gatcaaggcg gaaactcagt gctcgaagga gattcgagac gagtaagtcg aattgtgcgg
     2461 aatttattgg ctaatgcgat agaacatgct gagcagaaac caatcgaagt gaaagtcaaa
     2521 gctaatgagt cagcggttag tgttggagtc cgcgattacg gtcaaggtat gaatgaaacc
     2581 gatttggagc gtgtttttga tcgtttctgg agagctgatc cagcgaggca gcgtactttg
     2641 ggtggtactg gtcttggact ttcaatttct aatgaagacg cgaaattgca tcaaggtgag
     2701 ttaagagtct gggctaaacc gaatcaaggt gcgcattttg ttttaacttt gccattgcga
     2761 aacggtgaac caatagatat tttgccaatt aagtcaggcg aaaaatgaaa aaacttaagt
     2821 ttgtatttat tgcttttctt ttgacgggct gtgccgggat tccaacctct ggtcaaatca
     2881 agttcggtcc tgaaattaat tcaaatcagg cagaccaatt tattcaggtt attggccgtc
     2941 caccagcacc aggaatgact caagagcaaa tcgcccaagg atttcttgcc gcgcttgcag
     3001 actcgagaga tgattttgcg atagctaaag aatatttaac tgatcaaaca gctcgaactt
     3061 ggaagagttc tgcgggaatc actatttacg atccgaataa ttttcaactc tcggtagatg
     3121 acgcaattgc aaatataagt gcagcaaaat taggtgaaat tgattcgacc ggctatttga
     3181 cggtagcaag tgctagtgcc acagtaagct cacagtttcg ccttgttcaa gatttacaag
     3241 gtgaatggcg catctcggaa ctttctgacg gagttctact cacggtcaac gacatcgacc
     3301 gaagttttca aggttaccca atttactttc taggaccaga ccaaaaaact ctagttgcag
     3361 acacagtttt attacctcag acattaacgg gttctgctac agccttagtt caagcactac
     3421 ttgaaggtcc gtcaaaaaaa cttgattcag cagtacttag ttcatttcca accggaaccg
     3481 ctttaactta tgggtcggtg ccggtcacgg aaggtgttgc aaatatcgat ttaacaaatc
     3541 aaatacttag tgctgatcaa caaacaagat ctttaatgtc tgcacaactt gcttggacat
     3601 taagtacatt gccaaatgtt agtgctattt caatcactgt ttcaggtcaa ccaatttcaa
     3661 tagctggagt cacaagtgtg cagaccgtta gggattgggc acaatttaat ccagctcaat
     3721 tcactggaaa tgaaattttg cattatgtcc gtgacaatca agttttctct ttaaatttaa
     3781 atggcgaaga aactttagtt gttcaagtga atcctgcttc ccgtgtaaac ataagcaaag
     3841 cgtttggtgc accgcaaggt ggaagtattg cagcggtatc aacagacagt aagcgggtat
     3901 tgctaagcac tgggcggggt ggtcagtttg agcaagttgc cacaggtgag gcaatctcaa
     3961 aaccaagctg ggataagtct ggtggaattt tttattctga ttatggagtt gggatatttg
     4021 aaatcaattc gaatcgtgaa gttcgaccag taacttttga aagcaacgca ttggcttcgg
     4081 tgagccaggt taaacagatt gccgtggcaa aagacgggat tcgcgttgca atggtggttt
     4141 cagatggatc catcgactta ttaataggtg gggcaatttt taaatcaaca gaggcaactg
     4201 tgatttcggg cctacatcta attgaacgaa atataaatgc gatcaaagat ttaacttggc
     4261 aaacgccg
//
LOCUS       DBNHVN010000061         1071 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1274553, whole genome shotgun sequence.
ACCESSION   DBNHVN010000061 DBNHVN010000000
VERSION     DBNHVN010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1071)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1274553"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1071
                     /locus_tag="AC16Y1_01130"
     CDS             <1..>1071
                     /locus_tag="AC16Y1_01130"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR5831099.1"
                     /translation="LVKPDGLLANFDQQLPFKLTAGQQEVTKTIETELANGHPMHRLL
                     QGEVGSGKTVVALRAMLTVVDSGGQAALLAPTEVLATQHYRAIIKLLGPLAERGRLGA
                     HDEGTGVTLLTGSINTATRRQALLDITSGQAGIVIGTHALLEDRVDFFDLGLVVIDEQ
                     HRFGVEQRATLVAKAGQDRKPHVLVMTATPIPRTVAMTAFGALDVSTLTELPAGRSPI
                     STHVVLAAEKPSHLMRAWERVVEEVQKGHQAYVVVPRIASQEDKQSEKNSRPAVALED
                     LAPQLIDGPLKGIRVGILHGQMPGADKDEVMQRFTLGPTDPDGLDVLVATTVIEVGVD
                     VPNATMMVIMDADRFGISQLHQ"
BASE COUNT          300 a          234 c          273 g          264 t
ORIGIN      
        1 attggtcaaa cctgatgggt tattggcaaa ttttgatcag caactcccgt ttaaattaac
       61 ggccggccag caagaagtaa ctaagactat tgaaactgaa ttagctaatg ggcatccaat
      121 gcatcgatta ctgcagggtg aggttggttc aggtaaaacc gtggtagcac tgcgggcgat
      181 gctcaccgtg gttgattcag gtggccaagc agcattactt gcgccaactg aagtacttgc
      241 cactcaacat taccgagcga ttatcaaatt acttggacca ctggcagaac gtggtcgact
      301 tggtgcacat gacgagggga ctggcgtcac tttattaacc ggttcaataa acaccgcaac
      361 acggcgtcaa gcacttcttg atatcacaag tgggcaagct ggaatcgtaa ttggcacaca
      421 cgctctgctt gaagatcgag ttgatttttt tgatttaggt ttagttgtaa ttgacgaaca
      481 acatagattc ggcgttgaac aacgtgccac tttggtagcc aaggccggtc aagatcgcaa
      541 gccgcacgtg ctggtaatga cagctacccc aataccgaga actgttgcga tgaccgcgtt
      601 tggcgcactc gatgtttcaa cgttaacgga attacctgct ggccgaagtc caatcagtac
      661 gcacgttgta ttagccgctg aaaaaccaag tcacctaatg cgagcgtggg agcgagtagt
      721 cgaagaagtt caaaaaggac atcaggcgta tgtcgtggtg ccgaggattg cgagccaaga
      781 ggataagcaa tcagaaaaaa attctagacc ggcagtagca cttgaagatt tagcacctca
      841 attaatcgat ggcccattaa aaggtatccg agtaggtatt ctgcatgggc aaatgcctgg
      901 agcggataaa gatgaagtca tgcagcgctt cacgcttggt cccactgatc cggacggttt
      961 agatgtttta gttgcaacaa ccgtaattga agttggtgtc gatgtcccaa atgccaccat
     1021 gatggtaatc atggatgctg atcgatttgg cattagccaa cttcatcagt t
//
LOCUS       DBNHVN010000062         2272 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_11588, whole genome shotgun sequence.
ACCESSION   DBNHVN010000062 DBNHVN010000000
VERSION     DBNHVN010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_11588"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="AC16Y1_01135"
     CDS             complement(<1..422)
                     /locus_tag="AC16Y1_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020120051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HIR5831100.1"
                     /translation="MARPVTDLQRTVAPFEVISEFKPSGDQPKAIAELARRIERGDRD
                     VVLLGATGTGKSATTAWLIEKLQRPTLVIAPNKTLAAQLANEFRELLPNNAVEYFVSY
                     YDYYQPEAYIPQSDTFIEKDSSVNEEVERLRHSATNSL"
     gene            complement(432..1013)
                     /gene="coaE"
                     /locus_tag="AC16Y1_01140"
     CDS             complement(432..1013)
                     /gene="coaE"
                     /locus_tag="AC16Y1_01140"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR5831101.1"
                     /translation="MLVALTGGIASGKTTVAQLFAKKGAVVIKADDLARQVVAKGGRA
                     HDQVVAKFGPQILNADGEINRQLLADLVFANPEQLRILEELTHPAVQAELADQISDLP
                     LTQLIIYEIPLLIEKNLINKFDKSIAVIADLEIRETRAVERGLSPTDFYARVENQTSD
                     EQRMALVDIVIFNNGSLSQLAQQVDEAFAKLTS"
     gene            complement(1088..>2272)
                     /gene="rpsA"
                     /locus_tag="AC16Y1_01145"
     CDS             complement(1088..>2272)
                     /gene="rpsA"
                     /locus_tag="AC16Y1_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014846907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR5831102.1"
                     /translation="IEALVLQKEDKEGRLILSKKRAQYERAWGDIEKVKEQDGVVTGT
                     VIEVVKGGLIVDIGLRGFLPASLVEMRRVRDLAPYIGKQMEAKIIELDKNRNNVVLSR
                     RAYLEQSQSAVRTGFLNQLEPGQIRKGVISSIVNFGAFVDLGGVDGLVHVSELSWEHV
                     DHPSKVVEVGQEVTVEVLEVDFERERVSLSLKSTQEDPWQHFARTHQMGQVVPGEVTK
                     LVPFGAFIRVSDGIEGLVHISELSGAHVEIPEQVVQVGDQVFVKVIDIDLERRRISLS
                     LRQANDDVQVAGADTFDASVYGFNQYDEKGNYIYPEGFDSQTNEWLPGYEAQKEEWER
                     KYAEAHAKWEAHKAQAEEAKKAGGAAESAAATQSGESANEGTLSGDESLQALRDELSD
                     KA"
BASE COUNT          572 a          540 c          497 g          663 t
ORIGIN      
        1 aacagactgt tcgtggcact gtgccgaagt cgctctactt cttcgttgac tgatgagtct
       61 ttttcgatga aagtatcact ttgtggaata tatgcttcgg gttggtaata gtcgtaatac
      121 gaaacaaaat actcaaccgc attattagga agtaactctc taaactcatt tgctaactga
      181 gctgccaacg tcttattagg tgcgatcacc aatgtgggtc gttgtagttt ttcaataagc
      241 catgccgtag ttgcactttt acctgtaccc gtagcaccga gtaagacaac atctcgatca
      301 ccgcgttcta tccggcgcgc taattctgca atcgcttttg gctgatcacc gcttggctta
      361 aattcactga ttacttcaaa tggcgcaaca gtgcgctgta aatcagttac tgggcgggcc
      421 ataagtaaag gttacgaggt taacttagcg aaggcttcgt caacctgttg cgctaactgc
      481 gaaagtgagc cgttgttgaa aattacaatg tcgaccagtg ccatgcgctg ctcatctgac
      541 gtctggtttt ctacccgtgc ataaaaatct gttggggata gtccgcgctc aactgcccta
      601 gtttctcgaa tttctagatc agcgattaca gcgattgatt tatcgaattt gttaattaag
      661 ttcttctcga ttagtaatgg aatttcataa ataattagtt gcgtaagtgg taagtcgcta
      721 atttgatcgg ctaattctgc ttggacagct ggatgggtta actcttctag gattcgaagt
      781 tgctcgggat ttgcaaaaac taagtcagcc agaagttgcc gatttatctc accgtcagcg
      841 ttgagtattt gcgggccgaa ttttgcaacc acttggtcat gggctcgccc acccttagca
      901 acaacttgcc tagctaaatc gtcagctttg attacgacgg cacctttttt agcaaacagt
      961 tgagcgaccg tagtttttcc agaagcgata cccccggtta atgcgactaa cacataccaa
     1021 ggttagtacc ggaaaaacaa agagccacaa cctcgcggct gtggctcttt aacttgcttg
     1081 gcggtgttta agctttatcg cttaattcat cgcgaagtgc ttgaagtgat tcatcaccag
     1141 aaagagttcc ctcgtttgca gattcgccag attgagtagc tgctgctgat tcggcagcgc
     1201 cgccagcttt ctttgcttct tctgcctgtg ctttgtgggc ttcccactta gcatgtgctt
     1261 ctgcgtactt acgttcccat tcttctttct gggcttcgta acctggtaac cactcattag
     1321 tttgtgagtc gaaaccttct ggataaatgt agtttccttt ttcgtcgtac tggttaaacc
     1381 cgtataccga agcatcaaag gtgtctgcac cagcaacctg tacgtcgtca tttgcttgtc
     1441 gcagggaaag cgaaatacga cgacgttcta agtcgatgtc gatgacctta acgaaaacct
     1501 ggtcaccaac ctgaacaact tgttctggaa tttcaacgtg cgccccagag agttcagaaa
     1561 tgtggactag accttcgatt ccatcgctca cgcgaatgaa agcaccgaat ggaacaagct
     1621 tggtgacttc acctggaaca acttgaccca tctggtgggt acgagcgaaa tgttgccatg
     1681 gatcttcttg agtcgactta agcgatagcg atacgcgttc acgttcgaaa tcaacttcaa
     1741 gtacttctac tgttacttct tggccaactt cgacaacctt agatggatgg tctacgtgtt
     1801 cccatgaaag ttctgaaacg tgaactagac cgtctacacc gccgaggtca acgaaagcac
     1861 cgaagttaac aatgctcgag ataacaccct tgcgaatctg tcctggttct aattggttta
     1921 agaagccggt acgtacagca ctctgtgatt gttctagata cgcacgacga gataacacca
     1981 cgttgttgcg gtttttatca agttcaataa tttttgcttc catctgctta ccaatgtatg
     2041 gcgcaagatc acggacgcga cgcatttcaa caagtgacgc tggtaagaag ccacgaagac
     2101 caatgtcaac aatcaggcca cccttaacaa cttcgataac ggttccagta acaacaccgt
     2161 cttgttcttt aactttttcg atgtcacccc aggcgcgttc gtattgtgcg cgcttcttag
     2221 aaaggattaa gcgtccttct ttatcctctt tttgaagaac taaagcttcg at
//
LOCUS       DBNHVN010000063         3388 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1423631, whole genome shotgun sequence.
ACCESSION   DBNHVN010000063 DBNHVN010000000
VERSION     DBNHVN010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1423631"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..600
                     /locus_tag="AC16Y1_01150"
     CDS             136..600
                     /locus_tag="AC16Y1_01150"
                     /inference="COORDINATES: protein motif:HMM:NF014617.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR5831103.1"
                     /translation="MSSVFEAELTAKLAPVVAQVNLELDELSVSRSGKYRVLEIAIDG
                     DQVDLAAISVATKRISEYLDETNLMGEQQYTLEVTTRGIDRPLVKPQHWQRNIGRLVE
                     IKSDEFSGVGRIKSFADPLVRIETKDKTAEFDISLISQAIVQVEFSKPNEQG"
     gene            631..1611
                     /gene="nusA"
                     /locus_tag="AC16Y1_01155"
     CDS             631..1611
                     /gene="nusA"
                     /locus_tag="AC16Y1_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR5831104.1"
                     /translation="MDIDISALKAITKEKGFPLEYVVEAIESALNVAYQHSTGAHENS
                     RVHLDRSTGHVAVFAQDVDEEGKILREFEVTPEGFGRMAAGLARGVIAQRLKAADDEA
                     RVIEFQAKEGDIVSGVIAQGADRNLVFVNLGPVEGVLPPEEQVPGEDYEHGKRIRCFV
                     VSVKKGPKGPIITLSRSHPKLVEELFKFEVPEIADKVVVIEGLAREAGHRTKIAVSTS
                     TPGINPKGACIGPMGGRVRAVMSELGGEKIDIVDYSEDLAAYVTHALSPAKVVSATVL
                     DPINRVVRVVVPDYQLSLAIGREGQNARLAARLTGAKIDIKSDTAADLTP"
     gene            1879..>3388
                     /locus_tag="AC16Y1_01160"
     CDS             1879..>3388
                     /locus_tag="AC16Y1_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HIR5831105.1"
                     /translation="MSKVRVYELAKALGIESKEIIAKLEEVGEYVKSASSTVEAPVVR
                     RLHEKFPELKKSAEKAPAKTAAKKAPAKKAAAKAPSEDSVQETEVAQKPAEMKTSPTK
                     PTQAPDEPPAAKDSTSAVNPQAPATPKPATPGPRTGGARPGNNPFVSAPRPPRPGNNP
                     FAPAADRRDSGVPGAPRPPRPAGSGAGGPRPGGPRPGGPRGDRPGGFGAGVPRGDRPA
                     GFGGPRPGGQRPGGFGAGAGTGVPAGPARPGGRPGGPGGRGNAAGAFGKGGGKSSKNR
                     KSKRAKRQEFDNMMAPVMGGVALPMGQGQVLRLARGCSLTDFAEKIGTEPAALVQLLF
                     KLGEMVTATQSVNEETLEILGAELNYEIQIISPEDEDRELLESFDLEFGDEGGEADLE
                     IRPPVVTVMGHVDHGKTRLLDAIRESNVVSREAGGITQHIGAYQIHHMHEGINRAITF
                     IDTPGHEAFTAMRARGAQVTDIAVLVVAADDGVKPQTIEALNHAKAAEVPIVV"
BASE COUNT          955 a          726 c          852 g          855 t
ORIGIN      
        1 gattttcatg gtgatgatta ctgtcttaat tattcgattc ataatcacat tgcagtaact
       61 tctgtcctag aaatctgcac cctaattggg gtaaggtcaa cttataactg caagcccaaa
      121 ttgaaaaggt aagaaatgtc atcggtattt gaagcggaat taactgcaaa attagcaccg
      181 gtggttgccc aagtaaatct tgaattagat gaactttctg tctcgcgttc aggaaaatat
      241 agagttttag aaatagccat cgacggagat caagttgact tggctgcaat ctcagtggct
      301 actaaacgca tttctgaata tcttgatgaa acaaacctaa tgggtgagca gcagtacacc
      361 ttagaagtaa caactcgggg gatcgatcga ccgttagtta aaccacaaca ctggcagcga
      421 aatataggtc gcttggtcga aattaaatcc gatgaatttt ctggtgttgg tcgaattaaa
      481 agtttcgctg atccattggt cagaatcgag acaaaagata agacagctga atttgatatt
      541 tcactgatta gccaagcgat tgttcaggtt gaattttcta agccaaacga gcaaggctag
      601 gtatggatat ttaggtatgg atatttaggt atggatattg acatctcagc actcaaggca
      661 atcacaaaag aaaaaggttt tccgctcgaa tatgtagtcg aggcaattga gtctgcacta
      721 aatgttgcat atcaacattc cactggtgca catgaaaatt cgcgcgttca cctagatcgc
      781 tcaactggcc atgtggcggt atttgcccag gatgttgatg aagaaggaaa gatacttcgc
      841 gaatttgaag taacccctga aggtttcgga cgaatggcag ctggcttagc tcgcggagtg
      901 attgcgcaac gcttaaaagc cgcagatgac gaagcaagag taattgagtt tcaggctaaa
      961 gaaggtgaca tcgtttcagg tgttattgca caaggtgctg atcgaaattt agtttttgtt
     1021 aacttaggtc cagtcgaagg tgtacttcct cctgaagagc aagttcctgg tgaagattat
     1081 gagcatggga aacgaattcg ttgctttgta gtttcggtta aaaaaggtcc aaaaggtcca
     1141 atcattactt tgtctcgtag ccatccaaag ttagttgaag aattatttaa attcgaagtg
     1201 cctgagattg ctgacaaagt tgtagtaatt gaaggtctcg ctagagaggc tggccaccga
     1261 acaaagattg cagtttcaac ttcaacgcct ggcataaatc caaagggtgc gtgtatcggt
     1321 ccaatgggag gtcgggttcg cgccgtcatg agcgaacttg ggggcgagaa gatagatatt
     1381 gtcgattaca gcgaagactt agcagcctat gtaacgcatg cactttctcc ggcaaaagta
     1441 gtttcggcca ctgtgcttga tccgatcaat cgagttgttc gagtagttgt acctgattat
     1501 caattgagtt tggcaatcgg tcgcgaaggg caaaatgccc ggttagccgc acgtttaact
     1561 ggcgccaaaa tcgacataaa gtcagacacc gcagcagatt taactcccta aacccttatt
     1621 tctctgggtg aattgggaaa acaaggctct tggggcgtaa agttagagga agtgtgctgg
     1681 ctaattacac atttatatat tgtgtgcttt tccagcaggc tacaaagtaa ccgttactac
     1741 cgaaacatcg ggagtttaaa tgcaaaccaa aaaggttttg ttctaaccat gaacgcctta
     1801 aagcagtgtg ctcagtaatg agtgcgaaga aaattacggt tcggtaatta attagtttcc
     1861 ggaccagact ggagaaaagt gtccaaggtc cgggtttatg aactcgctaa agctctcggt
     1921 attgagagca aagagattat cgccaaactt gaagaagttg gtgagtatgt taagtcagca
     1981 tcatcaacgg tagaagctcc cgttgttcgt cgcttacatg aaaaatttcc tgaattaaaa
     2041 aagtccgcag aaaaagcacc tgccaaaact gcagccaaaa aggctccagc taaaaaagca
     2101 gcagctaaag cgccaagcga agattcagtt caagaaacag aagttgcgca aaagccggca
     2161 gagatgaaaa cgtcgccgac aaaaccaact caggctcctg atgaaccacc agcagcaaaa
     2221 gattcaactt cagcggtaaa cccgcaagcg ccagcgacac ctaaacctgc aacaccaggt
     2281 cctcgtacag gtggcgcacg tccaggtaat aatccattcg tttctgcgcc acgtccacct
     2341 cgtcctggca acaatccgtt tgctcctgct gctgatcgtc gtgactctgg tgttcctggt
     2401 gcgccacgtc cacctcgtcc tgccggtagc ggcgctggtg gtccgcgtcc aggtggtccg
     2461 cgtccaggtg gtcctcgtgg tgatcgacca ggtggcttcg gtgcaggtgt acctcgtggt
     2521 gatcgtccag caggttttgg tggcccacgt ccaggtggtc aacgaccagg tggcttcggt
     2581 gcaggtgcag gtaccggtgt gccagcaggt ccagcacgtc caggcggtcg tccgggtgga
     2641 cccggtggtc gcggtaacgc agctggcgca tttggcaaag gcggcggtaa atcttccaag
     2701 aatagaaaat caaaacgcgc aaagcgtcaa gaattcgaca acatgatggc accagtaatg
     2761 ggtggcgttg cgctgcctat gggacaaggc caagttttgc gattggctcg tggttgttcg
     2821 ttgactgatt ttgcagaaaa aattggaacc gagccagctg cactcgtaca gttgctgttc
     2881 aagttaggtg agatggttac tgccactcaa tctgtcaacg aagaaactct tgaaattctt
     2941 ggcgctgaat taaattatga aatacaaatt attagcccag aagatgaaga tcgcgaacta
     3001 cttgaatctt tcgatctcga atttggtgat gagggtggcg aagccgatct agaaattcgc
     3061 ccgccagtag ttactgtcat gggtcacgtc gatcatggta aaactcgact ccttgatgcg
     3121 attcgcgaat ccaacgttgt atcgcgtgaa gccggtggga ttactcagca cattggtgct
     3181 tatcaaattc accacatgca cgagggaatc aatcgcgcca tcacgtttat tgatacacct
     3241 ggtcacgaag catttaccgc tatgcgtgct cgaggtgctc aggtaactga catcgcggtt
     3301 cttgtagtag ccgcagatga cggtgtgaaa cctcaaacaa ttgaagcctt aaaccacgca
     3361 aaggctgcag aagtgccaat agttgttg
//
LOCUS       DBNHVN010000064         5344 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1497534, whole genome shotgun sequence.
ACCESSION   DBNHVN010000064 DBNHVN010000000
VERSION     DBNHVN010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1497534"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..3800
                     /gene="dnaE"
                     /locus_tag="AC16Y1_01165"
     CDS             240..3800
                     /gene="dnaE"
                     /locus_tag="AC16Y1_01165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR5831106.1"
                     /translation="MSNDSFVHLHVHTEYSMLDGAARLDNLFEKAAELGQPAIAMSDH
                     GNLFGAFEFWKKGSATNVKPIIGLEGYYAPQGRKERAPFDFGLAAIDDTSIDSAGRGR
                     YNYTHMTIWAENNEGLHNLFKLSSLASLEGFYYQPRFDAELLAQYGKGLIGTTGCPSG
                     EVNRWLQAGNYDQALKTAATMREILGKDNYFVELMDHGIGIERQTREGLLKIAKELNL
                     PLVATNDLHYVYPEDALMHEVLLCIGTRTTMDDPKRFKFDAQDFYLKSSAEMRDLWRD
                     FPDACDNTLLIAERCNVSFTEGQNLLPSFPVPAGETENTWLVKEVMVGLDRRFGQGKV
                     PAEHLTRAKYELQVVEQMGFPGYFLVVADLCRHAREQGIRVGPGRGSAAGAVIAWALG
                     ITELDPIEHGLLFERFLNPERISMPDIDIDFDERRRSEMIRYATQKYGEERVAQIVTY
                     GTIKAKAAVKDATRALGFPYALGDKVTKAMPPAVMGKDIPLYGVFDPEHPRYNEAGEI
                     RDLYEDDADIRAIVDTARGIEGLKRQPGVHAAGVILCREPLLDVIPVWRREQDGAVIT
                     QFDMGACESLGLLKMDFLGLRNLTVLDDALANIKINRGKTVVLEDLGLDDKKTYELLA
                     RGDTLGVFQLDSGPLRSLLRSMVPDSFEDISAVIALYRPGPMGANAHNDYADYKNKRK
                     VQVPIHTELAEVLDSILGDTYGLIVYQEQVMSIAQKLAGYTLGQADLLRRAMGKKKAE
                     ILEKEYKPFAAGMKERGFSDDAISTLWDKLVPFSDYAFNRAHSAGYGLVSYWTAYLKA
                     NYPAEYMAALLSSVKDDKDKSAVYLNECRRMGIKVFPPDVNESDVEFTAIGNDIRFGL
                     SAIRNVGVNVVESLKVSREQKGKFTSFTDFLTKVDIQVCNKRVVESLIKAGAFDSLGH
                     SRKGLMAIHLEAVDAILETKKQEAIGQFDLFGSAGTAGNQSVGGVDLTIDDEEWDKKF
                     LLTQEREMLGLYVSDHPLFGLEHVLATNTSMPISGVREANDGSVVTLGGLVTEVQMKT
                     TKRSGERWAIIALEDLEGVAEVLVWPKMFAQVGHLLIEDAVVLVKVRIDKRDDEAPRV
                     AALEVSVPDISAAQSGPVKLYLPESRINPAVMDSLRQILQTHKGTTEVHLTLIQDNSL
                     ISLKTDDKLRVNPSTSFFADLKALLGSGCLEAPKLTTAAN"
     gene            complement(3804..4271)
                     /gene="ybaK"
                     /locus_tag="AC16Y1_01170"
     CDS             complement(3804..4271)
                     /gene="ybaK"
                     /locus_tag="AC16Y1_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007394620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HIR5831107.1"
                     /translation="MSTPATKLLTSLGINFKIHEYEHDPSAESYGLEAADKLGYPYEQ
                     VFKTLVATTDSGFAVAIVPVNQQVSLKNLAHVLGSKKTQMAKPEVASRLTGYVVGGIS
                     PVGQKKLLPTVIDEIAQLQETVLVSGGKRGFDIELATLDLAKVTKAIFAAIAE"
     gene            4357..5109
                     /gene="priA"
                     /locus_tag="AC16Y1_01175"
     CDS             4357..5109
                     /gene="priA"
                     /locus_tag="AC16Y1_01175"
                     /EC_number="5.3.1.16"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004167694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase/phosphoribosylanthranilate isomerase
                     PriA"
                     /protein_id="HIR5831108.1"
                     /translation="MQSLLLLPAVDVANGQAVRLVQGDADSATEYGDALSVALDWQSA
                     GTTWIHLVDLDAAFGRGSNRELINDVIGKLDVNVQLSGGIRDEESLQAALKTGATRIN
                     LGTAALEDPTWTSKVIKEYGDRIAIALDVRGSVLAARGWTRDGGDLWETLERLESDGC
                     ARYVVTDVTKDGTLRGPNIELLLQVLAQTNKPVIASGGVAELSDLVALRALVSSGLEG
                     AIAGKALYAGAFTLEQAIQVAGPQQSSSQVRE"
     gene            5112..>5344
                     /locus_tag="AC16Y1_01180"
     CDS             5112..>5344
                     /locus_tag="AC16Y1_01180"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835581.1"
                     /note="with component II, the glutamine amidotransferase,
                     catalyzes the formation of anthranilate from chorismate
                     and glutamine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIR5831109.1"
                     /translation="MTAPDLTKFKELAKNRRVIPVVRRLLADGETALNLYRKLTGQRA
                     GTFLLESAEHGLSWSRYSFIGVSSSAMLSEQNG"
BASE COUNT         1497 a         1129 c         1258 g         1460 t
ORIGIN      
        1 tcgtttacca gcgattacgc tcctgaattg gctcgcgcac tagaacttgc ccgcgaactt
       61 taatcataaa atcaccgagt tgtcggccaa ccaacgtttt gacttagctg ttgggtaatc
      121 tattgaagtg caccgccgca aatccggctt tccccgttgc actttgtaaa taccgtaatt
      181 taaatacagt aatttaaata agttctgtaa ttaaaccgtt gagaagttag aggctgaccg
      241 tgagtaacga ctcgtttgtg cacctacatg tgcacaccga gtattcgatg ctagacggtg
      301 ccgctcgact agataactta tttgaaaaag ctgccgaact agggcagccc gcaattgcca
      361 tgagcgatca tggcaatctg ttcggcgcgt ttgaattctg gaaaaaaggc agcgcaacta
      421 acgtaaagcc aataattggt ttagagggtt attacgcacc gcaaggccga aaagagcggg
      481 ccccatttga ttttggttta gccgctatcg atgacacttc aatagatagt gctggccgag
      541 gtagatacaa ctacacgcac atgacaatct gggccgaaaa caatgagggc ttacataatt
      601 tatttaagct ttctagtttg gccagtctcg aaggttttta ctaccaaccg cgatttgatg
      661 ccgaactgtt ggcgcaatat ggcaaaggtt taattggcac tactggatgt ccatcgggtg
      721 aagttaaccg ttggttacaa gccggcaatt acgaccaagc attaaaaaca gctgcaacta
      781 tgcgcgaaat attaggtaaa gataactact tcgttgaatt aatggatcat ggaattggca
      841 tcgaacgtca aactcgagag ggtttactca agatagctaa agaacttaat ttgccattag
      901 tagcaacaaa cgacctacat tacgtttatc ccgaagacgc cctaatgcat gaagtgctgc
      961 tgtgtatcgg tacccgcaca actatggatg atccaaagcg ctttaaattt gacgctcaag
     1021 atttttactt gaaatcttcc gccgagatgc gtgacttatg gcgagatttt cctgatgctt
     1081 gcgacaacac tttacttatt gctgaacgct gcaatgtaag tttcactgaa ggtcaaaatt
     1141 tattaccaag ttttccagtc ccagctggcg aaactgagaa tacttggtta gttaaagaag
     1201 tgatggtcgg gcttgatcgt cgttttggtc aaggtaaagt tccagccgaa catttaacgc
     1261 gggctaaata tgaattacaa gttgttgagc aaatgggatt tcctggctac ttcttggtgg
     1321 ttgccgattt atgtcgacat gcacgagaac aaggtattcg cgttggacca ggtcgtggct
     1381 cggcagcagg tgcggttatc gcttgggcac ttggcataac tgaacttgat ccaattgaac
     1441 acggcttact ttttgaacgc ttcttgaacc ctgaacgaat ttcgatgcct gatatcgaca
     1501 tagatttcga tgaacgtcgg cgctcagaaa tgattcgcta tgcaacacaa aaatatggcg
     1561 aagagcgcgt tgcgcagatt gttacttacg gcacgatcaa agctaaagca gccgttaaag
     1621 atgcaactcg tgcgcttggt ttcccatacg cactaggtga caaagttact aaagccatgc
     1681 caccagcagt catgggcaaa gatatcccgc tctatggcgt atttgatcca gaacatccgc
     1741 gctataacga agctggcgaa atacgtgatc tctatgaaga tgatgccgac attcgagcca
     1801 tcgtagatac tgctcgtggt atcgaaggat taaagcgaca accaggtgtc catgcagccg
     1861 gcgtaatact ctgtcgtgag cctttacttg atgtgattcc agtttggcgg cgtgaacagg
     1921 atggggcagt tatcacccag tttgatatgg gtgcctgcga atctctgggc ttattaaaga
     1981 tggacttttt gggactgcgc aacttaacag ttctagatga tgcactggcc aacataaaaa
     2041 taaatcgtgg caaaactgtg gtgctcgaag atcttggact tgatgacaaa aaaacttatg
     2101 agttactcgc tcgtggtgac acgctcgggg tatttcagct agacagtgga cctttgcgtt
     2161 cactacttcg ttcaatggtc ccagactcat ttgaagatat ttctgcagta atcgcgcttt
     2221 atcgacctgg tccaatgggt gcgaatgcac acaacgatta cgcagactat aaaaacaagc
     2281 gcaaagttca agtgccaata cacactgagt tagctgaagt acttgattca attctgggtg
     2341 acacctacgg actcattgtt tatcaagagc aagttatgtc gatcgcccaa aaacttgcag
     2401 gatacacact tggtcaagca gatttacttc gccgagcaat gggtaaaaag aaagccgaaa
     2461 ttcttgagaa ggaatacaaa ccctttgccg ctggcatgaa agagcgtggc ttctcagacg
     2521 atgcaattag tacgttgtgg gacaaattag ttccgttctc ggactatgca tttaatcggg
     2581 cacatagtgc tggttatgga cttgtttctt actggactgc ctacctgaaa gccaattacc
     2641 cagctgaata catggcggct ttgctatcaa gtgtcaaaga tgacaaagat aaatcagcgg
     2701 tttacttaaa tgaatgtcgc cgaatgggaa taaaagtatt cccaccggat gtaaatgaat
     2761 cggatgttga gttcactgca attggaaatg atattcgctt cggcctatcg gccattcgaa
     2821 atgttggagt caacgtagtt gagtcactca aagtcagccg tgaacagaaa ggtaagttca
     2881 cttcatttac cgacttttta acgaaagtag atattcaagt ttgtaataag cgggtagttg
     2941 agtcgttaat taaagctggg gcatttgatt ccctcggcca cagccgcaaa ggcttgatgg
     3001 ctatccacct tgaagcagtc gatgcgattt tagagactaa gaaacaagaa gctatcggcc
     3061 agttcgattt atttggatca gctggcactg ccggcaacca aagtgtaggc ggagtagacc
     3121 taacaattga tgacgaagag tgggataaga aatttttact tacccaagag cgagaaatgc
     3181 tcggtttata tgtaagtgac catccactgt ttggcctaga gcatgtgttg gcgacaaata
     3241 cttcaatgcc aatttctggg gtgcgcgaag ctaacgacgg ttcagtggta acacttggtg
     3301 gcttagtcac tgaagttcag atgaaaacga caaaacgttc aggtgaacgc tgggcaatta
     3361 ttgcgcttga agatctcgag ggtgtggctg aagtattggt ttggccaaaa atgtttgccc
     3421 aagttggcca cttacttatt gaagatgcag ttgttttagt caaggtgcga attgataagc
     3481 gcgatgatga agcacctcgc gtagcagcac ttgaagtttc agtaccagat atttcagctg
     3541 cgcaatctgg accggtgaaa ctttatcttc ctgaatctcg aataaatccg gctgtgatgg
     3601 attcacttcg gcagattttg caaactcata aaggcacaac tgaagtgcac ttaactttga
     3661 tccaagataa ttcgctgatc tcattaaaga ctgatgataa attgcgcgta aatccatcga
     3721 cttcgttttt tgcagactta aaagcattac ttggctcagg ttgcctggag gcaccaaaat
     3781 taactacagc tgctaactag ttttcattct gcgatagctg caaaaattgc ctttgtaact
     3841 tttgccagat ctaaagttgc taattcaatg tcaaaccctc gcttgccccc acttactaaa
     3901 acagtttctt gtaattgggc aatttcatcg atcacagtgg ggagtaactt cttttggcca
     3961 actggactga tgccaccaac tacgtaacca gttagtcgac tggctacttc aggttttgcc
     4021 atttgtgtct ttttacttcc aaggacgtgc gctaaatttt ttaaactaac ttgctgattt
     4081 actggcacaa tcgccacggc aaatcctgaa tcagttgtag cgaccagagt tttaaaaact
     4141 tgctcgtagg gataaccaag tttgtcggca gcttctaatc cataagactc tgcgcttgga
     4201 tcatgttcgt actcgtgaat tttaaaatta atccccagac tggttagtaa cttagtcgct
     4261 ggagtgctca caaaagaatg ataagcgctc cgcccattag aattaggaag actaaatcaa
     4321 gccccatatc tcttagtaat ccaaaggttg gtagtaatgc agtcgttatt actcttgcct
     4381 gcggttgatg tggctaacgg acaagctgtt cggcttgttc aaggtgatgc agatagcgca
     4441 actgagtatg gcgatgcgct ttcagtggca cttgactggc aatcagcagg gacaacttgg
     4501 attcatctag ttgatttaga tgctgcattt ggccgcggtt caaaccgtga attaattaat
     4561 gacgtaattg gcaaacttga cgttaatgtt caactttcag gcggaatccg cgacgaagaa
     4621 tctttgcagg cagcattaaa aactggcgca actcgaatca acttgggtac ggccgcctta
     4681 gaagatccaa cttggactag caaagttatt aaagaatatg gcgatcgaat tgcaattgcc
     4741 ttagatgtac ggggaagtgt gctggctgca cgtggctgga ctcgtgatgg tggcgacctt
     4801 tgggaaactc tagaacgact agaaagtgat ggttgcgctc gttacgtagt tactgatgta
     4861 actaaagacg gcacattgcg tggtcccaac attgaattac ttctgcaagt tttagcccaa
     4921 acaaataaac cagttattgc cagcggtggc gtagccgaac taagcgactt agttgcgttg
     4981 cgagccttag ttagttcagg tctagaaggt gcgatcgccg gcaaagcact ttatgccggg
     5041 gcctttacgc tagaacaggc aattcaagtt gctggtcctc agcaaagttc tagtcaggtt
     5101 agggagtaaa aatgactgca cctgatttaa ctaagttcaa agagttagca aaaaatcgtc
     5161 gagtcatacc ggtagtccga cgacttctgg ccgatggcga aaccgcactt aacttgtatc
     5221 gcaagttaac tgggcagcgt gctggcacat tcttacttga atctgctgaa catggtcttt
     5281 cgtggtcacg ctattcattt atcggagtat caagctcagc catgctttct gaacaaaatg
     5341 gaca
//
LOCUS       DBNHVN010000065         1351 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_2018308, whole genome shotgun sequence.
ACCESSION   DBNHVN010000065 DBNHVN010000000
VERSION     DBNHVN010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1351)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_2018308"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="AC16Y1_01185"
     CDS             <1..802
                     /locus_tag="AC16Y1_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018960536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HIR5831110.1"
                     /translation="VVGISGIDTRALTRHLREQGAMRVGIFSGSQADEPVAQLLAKVK
                     ETPEMSGASFSQTVSTKATYVVPAVGQKKYQVAALDLGIKSMTPYLMAQRGIEVHVVP
                     ADISADELLNLGKDGVFVSNGPGDPASAAHAVALVQATLEAKRPFFGICFGNQILGRA
                     LGFGTFKLRYGHRGINQPVMDRTTGKVEVTAHNHGFAVDAPIDKDSQTKFGTVRVSHV
                     CLNDNVVEGLQCLDVPAFSVQYHPEAAAGPHDSAYLFDRFVDLMESSR"
     gene            802..>1351
                     /locus_tag="AC16Y1_01190"
     CDS             802..>1351
                     /locus_tag="AC16Y1_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase preATP-grasp
                     domain-containing protein"
                     /protein_id="HIR5831111.1"
                     /translation="MPKRSDIKSVMVIGSGPIVIGQACEFDYSGTQACRVLKAEGIRV
                     ILVNSNPATIMTDPEFADATYIEPITPEIVASIIERERPDALLPTLGGQTALNTAIAL
                     HKNGVLKKYDVELIGANVEAIERGENREKFRQIVADIGGQSAKSVICHTMEDCLAGVV
                     ELGYPVVVRPSFTMGGAGSGIAY"
BASE COUNT          378 a          284 c          323 g          366 t
ORIGIN      
        1 tgtagttgga atcagcggaa ttgatactcg cgccttaact cgtcacctac gcgaacaagg
       61 tgcaatgcgc gtgggtattt ttagcggttc acaagcagac gaaccagttg cacaactact
      121 tgccaaagtt aaagaaacac cagaaatgtc tggagcgagt ttctcacaaa cagtttcaac
      181 taaagcaact tacgtagttc ctgcggttgg gcaaaagaaa taccaagttg cagcccttga
      241 tttagggatc aagtccatga ccccatattt aatggcgcaa cgtggaatcg aagtacatgt
      301 agttcctgct gacatcagtg cagatgaatt acttaactta ggtaaagatg gggtatttgt
      361 ttctaatgga ccgggtgatc cagcgtcagc tgcgcacgct gttgcattag tccaagcaac
      421 gctagaagca aagcgaccgt tttttgggat ttgttttggc aatcaaatac ttggccgcgc
      481 acttggtttt ggtactttca aacttcgcta tggacaccgc ggaattaatc aaccagttat
      541 ggatcgcact actggaaaag ttgaagtaac tgcccacaat cacggcttcg cagtagatgc
      601 accaatagat aaagattcac aaactaaatt tggcacggtt cgggtaagcc atgtttgcct
      661 gaatgacaat gtggtcgaag gcttgcaatg tctcgatgtt ccagcattta gtgtgcagta
      721 ccacccagaa gcagctgctg gtccacatga ttcggcttac ttatttgatc gttttgttga
      781 cttgatggaa agtagccgct gatgccaaag cgcagtgaca tcaagtcagt aatggttatt
      841 ggttcaggtc caattgtgat tggccaagcg tgcgaatttg attattccgg aactcaagct
      901 tgccgagtgt taaaagccga aggtatccga gtaattctgg ttaatagtaa tccagcaact
      961 atcatgactg atcctgaatt tgctgatgca acttacattg agccaattac gccagaaatt
     1021 gtcgcgagca ttattgaacg cgaacggcct gatgctttgc taccaacact tggtggccaa
     1081 actgcgttaa atactgcaat tgctttacat aaaaatggtg tcctcaaaaa atacgacgtt
     1141 gaattaattg gtgccaatgt cgaagcaatc gaacgcggtg aaaaccgcga gaagttcagg
     1201 caaatagttg ccgacattgg tggtcaaagt gcaaagtcag ttatttgtca caccatggaa
     1261 gattgcctag ctggtgtagt tgaacttgga tatccagttg ttgttcgccc atcattcacg
     1321 atgggcggcg ctggctcagg tattgcttat a
//
LOCUS       DBNHVN010000066         1631 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1275338, whole genome shotgun sequence.
ACCESSION   DBNHVN010000066 DBNHVN010000000
VERSION     DBNHVN010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1275338"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..92)
                     /locus_tag="AC16Y1_01195"
     CDS             complement(<1..92)
                     /locus_tag="AC16Y1_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltetrahydrofolate cobalamin
                     methyltransferase"
                     /protein_id="HIR5831112.1"
                     /translation="MHTVLSSPNKTVTIGHDQPFVIIGERINPTG"
     gene            complement(106..1017)
                     /locus_tag="AC16Y1_01200"
     CDS             complement(106..1017)
                     /locus_tag="AC16Y1_01200"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0006555 - L-methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR5831113.1"
                     /translation="MSVLEHKLTKTNELVFTAEFPSLDGNGIEKAKKAADRMRGWFDG
                     VNATDNPAAHAHASNVASAIMMKQLGLEPIMQVVCRDKNRIAQQADIVGAAMFGIENF
                     SALTGDDVTAGDETEARRVFDLDGPQLIRLMSTLKRGQYMSGRKLDPAPHMFIGAVEN
                     PGAPPFEYRVQRVAKKYAAGAKFLQLQICYHKNRLEKFCAGVKAAGLADKIAIIPTIV
                     LVKGARPLHFMHDNVPGIDIPADEIARVEKASDPSEAAYQQTLEFAKHALAQPSVRGL
                     HVTDFRNDDTLDRLMTDLGRVRQVQTN"
     gene            complement(1018..1401)
                     /locus_tag="AC16Y1_01205"
     CDS             complement(1018..1401)
                     /locus_tag="AC16Y1_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017539409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR5831114.1"
                     /translation="MAEFIATIEHVIKGPVWGCQMCGQCVLHSTGMTCPMNCPKTLRN
                     GPCGGVRENGHCEVIPEMKCVWTKGYERSQAWPLPKLWKEEFNHLRAPVDNRLKGGSS
                     WKNFFSKRDRWTPKGWEETTNEAGH"
     gene            complement(1407..>1631)
                     /locus_tag="AC16Y1_01210"
     CDS             complement(1407..>1631)
                     /locus_tag="AC16Y1_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HIR5831115.1"
                     /translation="VGMSAFLTTTMPMFKVNIHEISKAGLRDDVIIMVGGAPVTQEYA
                     DVVGADGYAADASTAVRKAKELIAVKRGAK"
BASE COUNT          391 a          406 c          353 g          481 t
ORIGIN      
        1 cccgttggat taatgcgttc accgataatg acaaacggct gatcgtggcc gattgttacg
       61 gttttatttg gtgaactaag cacggtatgc atagtcattc ccttcttagt tagtttgaac
      121 ttggcgcact cggccaagat cggtcataag tctgtctaat gtgtcgtcat tgcgaaagtc
      181 tgttacatgc aatccacgaa cactgggctg agccagtgca tgcttggcaa actcaagagt
      241 ttgttgataa gcggcttcgg atggatcact tgctttttct actcgcgcaa tttcatctgc
      301 tgggatatca attcccggta cgttgtcgtg catgaagtgc aatggacgtg cacctttaac
      361 taaaacaatt gttggaataa tcgcaatttt gtcagccaag cctgctgctt taactccagc
      421 acaaaatttt tctaatctat ttttgtggta gcagatctgt aattgtaaaa actttgctcc
      481 agctgcatat ttttttgcca cacgttgcac ccgatattca aacggtggtg cacctgggtt
      541 ttcaacggcc ccgatgaaca tgtgcggagc tggatcgagt tttcgcccag acatgtactg
      601 cccacgcttt aaagtgctca ttaacctgat tagctgtggg ccgtctaaat caaatacccg
      661 acgggcttct gtttcatctc cagcagtaac gtcatcgcca gtaagtgccg aaaagttttc
      721 gataccaaac attgcggcac cgacaatgtc ggcttgttgc gctatccgat ttttgtctcg
      781 gcaaacaacc tgcataatcg gttcaaggcc aagttgcttc atcatgattg ccgatgccac
      841 gtttgacgcg tgggcgtgtg cagctggatt atccgtcgcg tttacgccat caaaccatcc
      901 gcgcatgcgg tcagctgctt tcttggcttt ttcaatccca ttgccatcga gtgatggaaa
      961 ttcagcagta aaaactaatt cgtttgtttt agttagtttg tgttccagaa cggacattta
     1021 gtggccagcc tcgttggtgg tttcttccca gcccttcggg gtccaacgat cacgcttcga
     1081 gaaaaagttc ttccacgatg atccaccttt taatcggtta tcaacaggtg cccgcaagtg
     1141 attaaactct tctttccaaa gttttggtaa cggccatgcc tgactgcgtt cgtaaccttt
     1201 agtccaaaca catttcattt ctggaattac ttcgcaatgt ccattttcgc gaacgccacc
     1261 acatggtcca ttgcgcagag tctttggaca attcattggg caggtcatcc ctgttgaatg
     1321 cagcacacat tgcccacaca tctggcagcc ccagactggg cccttgatta cgtgttcaat
     1381 tgttgcaatg aattcagcca tgagaactac ttagccccac gcttaactgc aattaattct
     1441 ttagctttac gcactgcagt tgaagcatca gcagcataac catcagcacc tacgacatct
     1501 gcgtattcct gagtcaccgg tgcaccacca accatgatga tgacatcgtc gcgcaaacct
     1561 gctttagaaa tttcatggat gtttactttg aacatcggca tggtagttgt taggaatgct
     1621 gacataccaa c
//
LOCUS       DBNHVN010000067         2147 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_680284, whole genome shotgun sequence.
ACCESSION   DBNHVN010000067 DBNHVN010000000
VERSION     DBNHVN010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_680284"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="AC16Y1_01215"
     CDS             complement(<1..790)
                     /locus_tag="AC16Y1_01215"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HIR5831116.1"
                     /translation="MDRVDAIVNLSKRRGFVYPSSEIYGGSRSAWDYGPLGVELKENV
                     KRQWWKSVVQGREDVVGLDSSVILAPAVWHASGHISAFVDPLTECTSCHKRFREDHLL
                     EEFEARKSRKPEGGLAEIACPNCGTKGQFTEPKMFNGLLSTNVGPVIDENSLHYLRPE
                     TAQGIFVNYANVAASSRKKPPFGIGQTGKSFRNEITPGNFIFRTREFEQMEMEFFVPP
                     GTDEEWHEYWLEERWNWYVDLGLSTDNLRRFEHPKEKLSHYSKRT"
     gene            complement(885..1220)
                     /locus_tag="AC16Y1_01220"
     CDS             complement(885..1220)
                     /locus_tag="AC16Y1_01220"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HIR5831117.1"
                     /translation="MTIGPFTSLWVFDERNLADWLAKAQIAIETLQALPGFIEAQAGR
                     SPDEPSRLIVTTKWEDVGSYRRALSSYAAKTSVWPFLANMHDQPSAFEVLLSADSAVF
                     SEYKSSLET"
     gene            complement(1217..1507)
                     /locus_tag="AC16Y1_01225"
     CDS             complement(1217..1507)
                     /locus_tag="AC16Y1_01225"
                     /inference="COORDINATES: protein motif:HMM:NF042772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6703 family protein"
                     /protein_id="HIR5831118.1"
                     /translation="MAKPLRKQIEILSTPILLRISSWPKAIVPLVLLAFLLVGLFMPG
                     VVGGLFILLVGLFVGWLLYLSWPLLAPGARILRLLAVTVILGSALTQIFLVK"
     gene            1629..>2147
                     /locus_tag="AC16Y1_01230"
     CDS             1629..>2147
                     /locus_tag="AC16Y1_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR5831119.1"
                     /translation="MAILVAGLTVLATLAGGLLAIRTKKNIHLVLGLSAGLLLGLVAF
                     DLLPEVFELTELEVGHVPAVMVAFLGGFLALHVLERSIGAHEPHNQVGSKDHSHKHNI
                     GGVIAASGMVIHIFLDGLAVALAFQIDNAFGWLVAIAVMTHAFTDGLNTVAMLRGSGN
                     WIGRAKLLLGADA"
BASE COUNT          581 a          499 c          472 g          595 t
ORIGIN      
        1 tggtgcgctt ggagtaatgc gaaagctttt cttttggatg ttcgaaacgt cgcaagttat
       61 cagttgatag accgagatcc acataccaat tccagcgttc ttcgagccag tactcatgcc
      121 actcttcatc ggtgcccggt ggaacgaaga attccatttc catttgttca aattcacgcg
      181 tgcggaagat gaaattaccc ggagtgattt cattgcgaaa cgactttcca gtttggccga
      241 taccaaatgg cggcttcttt cgagacgagg ccgcaacgtt tgcatagtta acaaaaatac
      301 cttgagcagt ttctggccga agataatgta aagaattctc atcgatgact ggaccaacgt
      361 ttgtagacag taagccattg aacatttttg gttcagtgaa ttgacccttg gttccacaat
      421 ttgggcaagc tatttcagca agtccgcctt cgggtttacg gctctttcgt gcttcaaatt
      481 cttcgagtaa atggtcttcc cgaaatctct tatgacaaga ggtgcactct gtgagtggat
      541 ctacgaatgc actgatgtgc cctgatgcat gccaaactgc cggagccaga attaccgaac
      601 tatcaagtcc aacgacatct tcgcgtcctt gaactacgct cttccaccac tgacgtttga
      661 cgttttcttt tagttcgaca ccaagcggac cataatccca ggcggagcga gatccaccgt
      721 aaatttcact ggatggatat acaaaaccac gtcgtttgga cagatttaca attgcgtcta
      781 cgcgatccat cgactcaaca atcacttaag ggctatccga ctgggtgccg gctttccgaa
      841 gtcttctaat tctagccaac taaaggctga ataaattgac tgtgctaggt ttccaaactt
      901 gatttgtatt cagaaaaaac cgcggaatcc gcactcaata aaacttcaaa tgcacttggt
      961 tgatcatgca tatttgctaa aaatggccag actgaagttt ttgcggcata actcgatagt
     1021 gcccgtcgat aggaaccaac atcttcccac ttagttgtaa cgattaatcg acttggttca
     1081 tctgggctgc ggccagcctg tgcttcgatg aatccaggta gtgcttgcaa agtctcaatt
     1141 gcgatttgcg ccttggctag ccaatcggca aggtttcgtt catcaaaaac ccaaagcgat
     1201 gtaaatggac caattgtcat ttaactaaaa atatctgagt cagcgcacta ccaagaatta
     1261 ctgtgacggc caataggcgc agaattctgg cccccggggc taatagcggc caagaaagat
     1321 aaagtaacca gcccacaaaa agcccgacta gcaaaataaa tagtccacca acaacgcctg
     1381 gcataaacaa accgaccagt aaaaaggcca acaaaacaag tgggacgata gcttttggcc
     1441 aactgctaat gcgcagcaaa attggcgtac tcagaatttc tatttgtttc cggagtggtt
     1501 ttgccatttg ttactgcctt attttcgctc gatttcgatg gctttatcct aattggaatc
     1561 aagtaaagcc ttattgacaa tgattcttat taagactaaa ttggaatcat tcccaataag
     1621 gagtttacat ggcgatatta gttgcgggcc taaccgtact ggccaccctc gctggcggct
     1681 tactggcaat tagaaccaag aaaaacattc acctagtgct tgggctaagc gcaggtttat
     1741 tacttggctt agttgctttt gatcttttgc ctgaggtttt tgaactaaca gaacttgaag
     1801 ttggccatgt gcccgccgtt atggtcgcat tccttggtgg ctttttagcc ctacacgttc
     1861 tagaacgttc tattggggcc catgaacctc ataaccaagt tggcagcaaa gatcactcgc
     1921 ataaacacaa cataggtggc gtaattgcgg caagcggaat ggttattcac attttccttg
     1981 atgggcttgc ggtagctcta gcttttcaaa tcgacaatgc atttggctgg ttagttgcaa
     2041 tcgctgttat gacgcacgca tttactgacg gccttaatac cgtcgcaatg ttgcgtggat
     2101 caggtaactg gattggtcga gcaaaattac ttttaggtgc cgacgca
//
LOCUS       DBNHVN010000068         1502 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1572533, whole genome shotgun sequence.
ACCESSION   DBNHVN010000068 DBNHVN010000000
VERSION     DBNHVN010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1572533"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="AC16Y1_01235"
     CDS             complement(<1..209)
                     /locus_tag="AC16Y1_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831120.1"
                     /translation="MESEEFEGESVLDVEIEELDEDLAQGPSKPAIALSTLPITGEPR
                     VDDALARLNEIPGLPVHEHVEVFEDV"
     gene            complement(214..1176)
                     /locus_tag="AC16Y1_01240"
     CDS             complement(214..1176)
                     /locus_tag="AC16Y1_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007540003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent bicarbonate transport family
                     permease"
                     /protein_id="HIR5831121.1"
                     /translation="MDSLSIAISNLTSVAVLIFVMGFIGARIKSDMRIPEPVYQMISI
                     FLLLGIGLKGGNSLKGVPGSEILVPSIVTVALGFLIPLIAFLTLRLIKSLDDTDRGSI
                     AAHYGSTSLVTFSAALLFLETNKIQVEGYATLLLTLLEIPGIIVGIYLGSRHLNQNVK
                     WASTLHEVVTGKTILLLVGGLIIGAVTSQSGYEKVSPFFVDLQNGFLTLFLLHLGFIA
                     GSNWIEIKKVGPSMAVFAVIFPVVAGALGVLAGNLAGLSVGGAAVLGVLSASASYIAA
                     PAAVAVGLPKANTSLALMASIGVTFPFNLVLGIPLYLEFANWLG"
     gene            complement(1169..>1502)
                     /locus_tag="AC16Y1_01245"
     CDS             complement(1169..>1502)
                     /locus_tag="AC16Y1_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HIR5831122.1"
                     /translation="DILGANNVGIDSLLVLSGVTTVAELLSAIPSERPTYLGWDAASV
                     LLPQPEVEVHGNEVHLAGFKVDRTGLSGAGDALDAIRVCAVAAWLEICPMEQLLDNLN
                     NRGINSRG"
BASE COUNT          456 a          372 c          313 g          361 t
ORIGIN      
        1 acgtcttcaa aaacttcaac atgttcatgt actggtaagc cgggaatctc gtttaatcga
       61 gcaagtgcgt catcaacgcg aggttcgcca gtaatcggca aagtcgacag tgcgatcgca
      121 ggtttacttg gaccttgcgc taaatcttcg tcaagttctt caatttcaac atcaagaact
      181 gattcgcctt cgaattcttc ggattccatg taactagcct aaccagttgg caaattctag
      241 ataaagcgga atacctaaca ccaagttaaa tgggaatgtc acaccaatac ttgccattag
      301 agctagtgaa gtatttgctt ttggtaagcc aactgctact gccgccggcg ctgctatgta
      361 ggaggcactt gcactaagta cgccaagaac cgctgcgcca ccaacagata agccagccaa
      421 attaccggcc aatacgccaa gtgcgccagc cacaactggg aagatcacgg caaacacggc
      481 cattgacggc ccaacttttt tgatttcaat ccagttactg ccagcaataa agccaaggtg
      541 aagtaagaac agcgttagaa acccgttttg cagatcaacg aaaaatggtg agactttttc
      601 atatcctgat tgcgaagtaa ctgcgccaat aattaagcca ccaactaaaa gcaatatcgt
      661 tttaccagtt accacttcgt gcagagtact tgcccactta acgttttgat ttaggtgccg
      721 tgaacctaaa taaataccaa cgatgatgcc tggtatttca agcagggtta gcaacaatgt
      781 cgcataaccc tcgacttgga ttttgttggt ttccaagaac aaaagcgctg ccgaaaaagt
      841 aactaaggat gttgagccat agtgcgcggc aatcgatcca cgatcagtgt catccaagga
      901 cttaattaac ctcaaggtta agaaagcaat cagtggaatc aaaaagccaa gtgcaacggt
      961 gacaatcgat ggcaccaaga tttcgctgcc gggcacacct ttaagtgagt taccaccctt
     1021 aagaccaata cccagtagca agaaaatcga gatcatttga taaaccggct ctgggatacg
     1081 catatcgctt ttgatccgag ccccaataaa gcccatcaca aagatgagta cggcgactga
     1141 agttaaattc gatattgcaa tcgaaagact atccacggct attaattcct cggttattta
     1201 aattgtctaa caattgttcc attggacaga tctcaagcca ggctgccaca gcgcaaaccc
     1261 ggatcgcgtc aagggcatcg cctgcgcccg ataacccggt tcgatcaact ttgaagccag
     1321 ctaagtgaac ttcattacca tgaacctcga cttcaggttg tggtagtaaa acactggctg
     1381 catcccaacc taaatatgtt ggccgttcac ttggaattgc gctcaacaac tcggcaaccg
     1441 ttgtaacccc tgacagtact aatagcgaat caatgccgac attgtttgcg ccaagaatgt
     1501 ca
//
LOCUS       DBNHVN010000069         1291 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_2018490, whole genome shotgun sequence.
ACCESSION   DBNHVN010000069 DBNHVN010000000
VERSION     DBNHVN010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_2018490"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="AC16Y1_01250"
     CDS             complement(<1..451)
                     /locus_tag="AC16Y1_01250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR5831123.1"
                     /translation="MAKKDAIETAKKPSADRNLVLTVGKESVLVSRVIDGVVTAARNA
                     DPNFIRQDLVASNENAAGDFANAMSPSLFNELTVLVINGIDSASDELAEQITTHLTKL
                     PDHIRLVLIHPGGVKGKRLLDQIRKAGAVEANCSELKGKDLESALTAE"
     gene            complement(494..>1291)
                     /locus_tag="AC16Y1_01255"
     CDS             complement(494..>1291)
                     /locus_tag="AC16Y1_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR5831124.1"
                     /translation="WQPPNKALTNWVPSNWQIVSCDVGQGDATLIRTARNEAIVVDVG
                     GDPKLINDCLTQVKVDRIPLLLLTHFHADHVVGLPGALANREVEQIRVSPLTDPPLTT
                     EFVYKVLSEFGKNTSVLAYPEYLKINDLEIFTIWPKARLNQASTTPNNASVSILVKTP
                     DLSVLLPGDVEPPAQNAIVDLVGTLKVDVIKIPHHGSRYQSADFAKAASPKVALVSSG
                     IGNSYGHPAPETIFMYELVGAKVLRTDRSGSIAIFSRGEKILTSTQR"
BASE COUNT          354 a          290 c          260 g          387 t
ORIGIN      
        1 actcggcagt taatgcgctt tctaaatctt tgcccttaag ttcgctgcaa ttggcttcaa
       61 ctgcacccgc tttacgtatt tgatctagca accgctttcc tttaacgcca cctggatgta
      121 ttaaaactaa gcgaatgtga tctggcaatt tggttaaatg tgtcgtgatt tgttccgcta
      181 actcatcgct tgcactatca ataccattga ttaccagcac ggtaagttca ttgaataatg
      241 atggcgacat tgcattggca aaatcaccgg cagcattttc atttgaagca actaaatctt
      301 ggcgaatgaa attcggatct gcgttgcgtg ccgcagttac taccccgtcg attacccgac
      361 taactaaaac agattcctta ccaactgtta aaactaagtt acggtcagca cttggtttct
      421 ttgcagtttc gattgcgtct tttttagcca caacctgagt ttgccacact cgatttgaaa
      481 aatttaagct tggctatctt tgggtactag tcaggatttt ttctcccctg gaaaagatgg
      541 caattgagcc tgagcggtca gttcgaagta cttttgcacc aactaattca tacataaaaa
      601 ttgtttctgg ggctggatgg ccataagaat ttccgatacc tgaagagacc agagcaacct
      661 tgggtgaggc agctttagca aaatctgcag actgatacct agatccatgg tgtggaatct
      721 taattacatc aacttttaat gtcccaacta aatcaacgat tgcattttga gcgggcggct
      781 ctacatcccc aggtaacagc acactcaggt cgggtgtttt gaccaatatc gaaacacttg
      841 cattatttgg agtggtactg gcttgattga gtcttgcttt aggccaaatg gtaaaaatct
      901 ccaaatcatt aatctttaaa tattctgggt aagccaagac cgaagtattt tttccaaact
      961 cactcaaaac tttgtagaca aattctgttg tcaagggtgg atcggttaat gggctcacac
     1021 gaatttgttc tacttcgcga ttagccaacg caccaggaag cccaacaaca tggtcggcat
     1081 gaaagtgggt taatagcaaa agcggaatcc gatcgacttt cacttgagtt aaacaatcat
     1141 ttattagctt aggatcgccg ccaacatcaa cgactatcgc ttcatttcta gctgttcgaa
     1201 ttaatgtggc atcaccctgt ccgacatcac acgagactat ttgccaatta ctcggtaccc
     1261 agtttgttaa agccttattg ggcggttgcc a
//
LOCUS       DBNHVN010000070         1482 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_532402, whole genome shotgun sequence.
ACCESSION   DBNHVN010000070 DBNHVN010000000
VERSION     DBNHVN010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_532402"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="AC16Y1_01260"
     CDS             <1..200
                     /locus_tag="AC16Y1_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831125.1"
                     /translation="LIMDVDIATATHTSTVAPVSAQSLTSSIGKQVYVGTVIESKAVV
                     VRQIGSSWTDVSIGFSPYFSE"
     gene            273..887
                     /locus_tag="AC16Y1_01265"
     CDS             273..887
                     /locus_tag="AC16Y1_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR5831126.1"
                     /translation="MIELLSDLLFTPLCLGCKKLGVNLCIACLDQLEIVNRTDLLGID
                     KLFCAGEYKHWLRDQVIAYKSGSYQNARGLAQILTFKCLNKLPSYPLVPIPSSEEKIK
                     IREIDTIGHLTKQIRFFSPSVQVLPALRLTKNLPDQVGLSKKERIGNLKDAFACVKPI
                     SKPVILIDDVVTTGATLISAAQTLLKHGAPKVYAVGLCATQKLR"
     gene            complement(1160..>1482)
                     /locus_tag="AC16Y1_01270"
     CDS             complement(1160..>1482)
                     /locus_tag="AC16Y1_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HIR5831127.1"
                     /translation="GYGGRREVVDAVKSLLKEHATAGSTLEELANMIDIEHIAEHLYT
                     KGQPDPDLVIRTSGEQRLSGFLLWQSAHSEFYFEEAYWPNFRKVDFLRALRSYAARHR
                     RFGI"
BASE COUNT          423 a          307 c          320 g          432 t
ORIGIN      
        1 atttgattat ggatgttgat attgcaacag ccacccatac gtcaactgtc gcgccggtaa
       61 gcgcacaatc tttaactagc tctattggca aacaggttta tgtaggaact gtaattgagt
      121 caaaagcggt ggtcgttagg caaattgggt cgagttggac tgatgtaagt attggtttct
      181 ctccctattt ttctgagtaa ctcacaccgc agtaatttac acaccttgaa ttcatattta
      241 ttttttttgg ataaatcgat ttcactcagt tagtgattga gttacttagc gatttattgt
      301 ttacacctct atgccttggc tgcaagaaac taggtgtcaa tctgtgtatt gcttgtcttg
      361 atcagctaga gatagtaaac cgaactgact tgcttgggat cgataagtta ttttgtgctg
      421 gtgaatacaa gcactggcta cgcgaccagg tcattgcgta taaatctgga agttaccaga
      481 acgctcgtgg tttggctcaa atattgacat ttaaatgttt gaataaatta cctagctatc
      541 ccttggttcc aattccgtct agtgaagaaa aaattaaaat tcgcgagatt gacacaattg
      601 gtcatctgac caaacagatt cgattttttt caccgtcggt tcaagttcta ccagcattac
      661 gcttaaccaa aaatcttcct gatcaagtgg gactttcaaa gaaagagcga attggtaact
      721 taaaagacgc atttgcttgc gtgaagccaa tatcgaaacc cgtaattctg attgatgatg
      781 tagtcacaac gggagcaaca ctaattagtg cagcccaaac acttctcaaa catggcgcac
      841 caaaggtcta cgcagtggga ttatgtgcaa ctcaaaaatt gcgttaactt attactacgg
      901 catccacctg ggtccgtgat tgcgtttaac aagtaaacct tcgaagatta tttgttaaac
      961 aagccgatgc cagccgcagg caaagcggtc cacgtaagac gactcttggt cgttgagcac
     1021 ggtgcggttt agaagtaaga cctgcctgat aagtacgtaa ttatttatta gcgagggtta
     1081 gtagtggtga attgacgctg cgaaaattcc agcaggcgat tgtggggtcg aaaatcaggt
     1141 cggccaggtg gatgtcttat taaattccaa atcttcgatg tcgggctgcg tatgaacgca
     1201 gggctcgcag aaaatcaact tttcgaaaat tcggccagta agcttcctca aaataaaact
     1261 cagaatgagc actttgccag agcaaaaatc cactaagtct ttgttcgcct gatgttctaa
     1321 tcaccagatc aggatcaggt tggccttttg tgtacaagtg ctcagctata tgttcaatgt
     1381 cgatcatgtt ggccaactct tcaagagtcg atcctgcagt tgcatgctct ttcaataaag
     1441 acttaactgc atcaacgact tcacggcgcc caccgtaacc aa
//
LOCUS       DBNHVN010000071         1489 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1424470, whole genome shotgun sequence.
ACCESSION   DBNHVN010000071 DBNHVN010000000
VERSION     DBNHVN010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1424470"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="AC16Y1_01275"
     CDS             <1..940
                     /locus_tag="AC16Y1_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006946416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR5831128.1"
                     /translation="PVAQVVRSGFIEGRHFGSAVVVDSTGKVIWSTGEVATQIFPRSS
                     NKLMQGLAMVRAGLPAKGELLALACASHSAESIHLEGVQSLLNAAGLTTDALRCPADW
                     PLDPVEKDTLLAEGIEKENIYMNCSGKHAAMLFTCVINSWDTRNYLEIDHPLQKLCKE
                     VIEEVTGEAVEQTGVDGCGAPVMSTSLIGLARAFGKFAGPAADDQQKLIAEAIRSHPE
                     FLGGTRRDVTKLIKGVPGLVAKDGAEGVYAIGLGDGRALAVKIEDGSDRARIVVANSL
                     LKNVLNVVSEEVDRQLTSQVLLGGGKPVGAVEALVP"
     gene            complement(953..>1489)
                     /locus_tag="AC16Y1_01280"
     CDS             complement(953..>1489)
                     /locus_tag="AC16Y1_01280"
                     /inference="COORDINATES: protein motif:HMM:NF022102.7"
                     /note="non-specific DNA-binding; scans chromosomes during
                     sporulation for DNA-damage; delays initiation of
                     sporulation; participates in a checkpoint signaling
                     cascade for cell-cycle progression and DNA repair; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HIR5831129.1"
                     /translation="VSIRDVAIVIQRHEMVRRISSEISNYVVSLGSEGRLISLQLEEL
                     VAGTDEELRLVIRDYMPAGRRQKNPENYLTALAALDATSILDLGAVAGALGLPSDVES
                     ADQHLSPRGFRLLAKVPRLPELIIENLTSHFNGLQRLLAASAEELRDVEGVGETRARA
                     IRESLSRLAEVSLAERYS"
BASE COUNT          403 a          322 c          373 g          391 t
ORIGIN      
        1 gcctgttgct caagttgttc gaagcggatt tattgaaggg cgacatttcg gcagcgcagt
       61 ggttgtagat tcaactggca aagttatctg gtctacgggc gaagtggcga ctcagatatt
      121 tcctagatct agcaacaagt tgatgcaagg acttgcaatg gttcgggcgg gtttaccagc
      181 taaaggcgag cttcttgcgc ttgcttgtgc ttcgcacagt gctgagagca ttcatctcga
      241 aggggttcaa agtttattaa atgcagcagg acttacgaca gatgcattgc gctgcccggc
      301 agattggcca cttgatccag tggaaaaaga tactttgctg gcagaaggta tcgaaaaaga
      361 aaatatctac atgaactgca gcggtaaaca tgcagcgatg ctttttactt gcgtaataaa
      421 tagttgggat actcgaaact acttagaaat tgatcatcca ttacagaaac tgtgtaaaga
      481 agtcattgaa gaagtaaccg gagaagccgt tgaacaaaca ggcgtcgacg gatgcggtgc
      541 gccggttatg tctacttcat tgattggttt agcaagagca ttcggaaaat ttgccggacc
      601 ggctgcagac gatcaacaaa aattgattgc agaggcaatc cgaagccacc ctgaattttt
      661 aggtggtact cggcgcgatg ttacaaaatt aattaagggt gtgcccgggc tagttgctaa
      721 agatggggcc gagggcgttt atgcgattgg cctaggtgac gggcgagcat tggcagtaaa
      781 aattgaggat ggtagcgacc gagccagaat tgtggtggct aactcattac ttaaaaatgt
      841 attgaatgtt gtctccgaag aggttgaccg acaattaacg agccaagttt tgcttggcgg
      901 cggcaaacct gttggggcag tcgaggcttt agttccttaa gcgaacctaa agttacgagt
      961 aacgctcagc caaactaact tcggccaggc gtgacaaact ttcgcgaatc gctcgtgctc
     1021 ttgtttcacc aacaccctca acatcccgta attcttcagc gcttgcggca agtaatcgct
     1081 gcaatccgtt gaagtgtgaa gttaagtttt caattattaa ttcaggcaat cttggcactt
     1141 tagccaataa tctaaagcca cgcggactta aatgttggtc agcactttcc acgtcacttg
     1201 gtaaacctag ggctcctgct accgccccta gatccaagat cgacgtggca tcaagtgcgg
     1261 ccaaagcagt caagtaattt tctggatttt tttgtctacg acctgctggc atgtagtcgc
     1321 ggattactaa gcgcaattct tcatcggttc ccgcaactaa ttcttcaagt tgaagtgaaa
     1381 ttaggcgccc ttcagaccca agtgaaacaa cgtagttact tatctcactt gaaattctgc
     1441 gcaccatttc gtggcgttga ataacaatcg cgacatcgcg gatacttac
//
LOCUS       DBNHVN010000072         1167 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1126977, whole genome shotgun sequence.
ACCESSION   DBNHVN010000072 DBNHVN010000000
VERSION     DBNHVN010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1126977"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..776
                     /locus_tag="AC16Y1_01285"
     CDS             75..776
                     /locus_tag="AC16Y1_01285"
                     /inference="COORDINATES: protein motif:HMM:NF013989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR5831130.1"
                     /translation="MELDLTEASVAQLADFYYSSEKTLLRANMVMSQDGHFVDEFGSS
                     RGLSSQLDLKVLLTLRALSDAVLVGGKTVRTENYQTPRLRGEYTLLNSVAPKLVVLSK
                     SLDFDLASRLFANSDNPPIFITEESKSDSWQSNYLRLKTAAEIVPLPVPLNLSNVLDY
                     LHSNGLRQILSEGGPEVLEQLIGEDLVNELDITISPTKMGGPAEQTKVHHAIANWPNR
                     VSAKLGSHRVFRIKR"
     gene            788..>1167
                     /gene="msrB"
                     /locus_tag="AC16Y1_01290"
     CDS             788..>1167
                     /gene="msrB"
                     /locus_tag="AC16Y1_01290"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIR5831131.1"
                     /translation="MSNPKVNKTEQEWRAQLSPAEYQVLRESGTEAPFIGEYTDTETE
                     GVYRCRACDAELFRSDHKFHSGCGWPSFFTPLAKDSIIEITDKSHGMVRTEIRCASCD
                     SHLGHVFAGEGYETPTDLRYCINS"
BASE COUNT          351 a          226 c          266 g          324 t
ORIGIN      
        1 atgtgttcgg cctctagaac tggagtacga gaacacaaac tttgcatcac cccagcctat
       61 gcaaagattg caatatggag ttggatctaa ccgaggcaag tgtggcgcag ttagctgact
      121 tttattactc atctgaaaaa actctgctac gtgcaaacat ggtaatgagt caagacggtc
      181 actttgttga tgaatttggt tcgtcgagag gtttatcaag tcaacttgat ttgaaggtat
      241 tactaacttt gcgagcgtta agtgatgcag tcttggttgg tggaaaaacg gttcgaacag
      301 agaattatca aacacctcgt ttgagaggtg aatacacatt gttaaattcc gttgcaccaa
      361 aacttgtggt actgagcaaa tccttagatt ttgatttggc tagcagatta tttgccaact
      421 cggataatcc cccaatcttc atcacagaag aaagtaaatc tgactcttgg caaagtaatt
      481 atttaagact gaagaccgct gctgaaatag tgcctttgcc tgttccgctg aatttatcga
      541 atgttttgga ttaccttcat agcaacggat tgcgtcaaat tttaagtgaa ggtggaccag
      601 aggttctaga gcaattgatt ggcgaagatt tagttaacga attagacata acaatttctc
      661 cgacaaaaat gggtgggcca gccgagcaaa cgaaagttca ccacgcaata gcaaattggc
      721 ctaaccgagt gagtgcaaaa ttaggttcgc atcgcgtctt tcgaattaag cggtaaatta
      781 gttacttatg agtaatccaa aagttaacaa aaccgagcaa gagtggcgag cacaacttag
      841 tcctgccgag taccaagtgc taagagaatc gggaaccgaa gcgcctttta tcggtgaata
      901 cacagacaca gaaacagagg gtgtttatcg ttgtcgagct tgtgacgcag aattgtttcg
      961 aagcgatcac aagtttcatt caggatgtgg ctggccttcc tttttcaccc cgttagctaa
     1021 agattcaatt attgaaatta cggataaatc gcatggcatg gttcgaactg aaatcagatg
     1081 tgccagttgt gattctcact tgggacacgt tttcgctggt gaaggttacg aaactccaac
     1141 tgacttgaga tactgcatca actcaat
//
LOCUS       DBNHVN010000073         2669 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1871144, whole genome shotgun sequence.
ACCESSION   DBNHVN010000073 DBNHVN010000000
VERSION     DBNHVN010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1871144"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="AC16Y1_01295"
     CDS             complement(<1..909)
                     /locus_tag="AC16Y1_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR5831132.1"
                     /translation="MTSSTTSKKKPRARKLERDRVRNKTEGKKTSTRHEAAKKAASDK
                     RKKPAKKVKVRSDREVATIAKSSAPKSDRKKTKTKEVWNPSGKSTNKNSYRSAREARE
                     TREETRRPKSASRYSREDRFEKPSREKPAREKPSHERSEYKSSEREKPSRVKAVQDRP
                     IRQKATQENSRPKRTEKFERTDIRETRKSAVIKPVKEKEYEKLEPVDLPAWSELGVHE
                     NLLPVLKRQGIETPFPIQVKTLPDTIGGRDVLGRGQTGSGKTLAFGLAMLTKLAHRKA
                     TKNHPLGLVLVPTRELAMQVTDALKPL"
     gene            1004..2188
                     /locus_tag="AC16Y1_01300"
     CDS             1004..2188
                     /locus_tag="AC16Y1_01300"
                     /inference="COORDINATES: protein motif:HMM:NF014680.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR5831133.1"
                     /translation="MDALVIVGIAVVSVVIGLLAGRLFAKSVSGFAQLSADEIRRQVG
                     DAAREQLQAIAQSLETSTEQRLQTTTQSIAEQNRMASTSMEAMIKPLQETLKSLDTKI
                     IDLENRRIEAYTSVQEQVKTTNAALEALRSETTMLSGALRRSDTRGRWGELQLRRIIE
                     MMNMSEHISFSEQIQQQGEGSGKPDVTVYLPGNRVLYLDSKAPMSSYLDAINETDHIR
                     KSELLDSHAKALKAHVKTLSNRDYAKDEAALNYVIMFIPTESSLAAACEINPGLIEDA
                     AASGVVLTSPTSLVAVLSNISMLWQQDAQNRNADEILRSSQELHSRLSVFINHFTKIG
                     SNLKKSVESFNDAVSSFDTRIMKTATDIQKLGRYTDELPSVPKLEVEPKNSKYEQPEL
                     EN"
     gene            complement(2314..>2669)
                     /locus_tag="AC16Y1_01305"
     CDS             complement(2314..>2669)
                     /locus_tag="AC16Y1_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5831134.1"
                     /translation="ETNEFEDVQFEKGRVKNENGHVNDNLYVVGWAKRGPSGVIGTNK
                     SDSTEVMKFLVDELKAPKESDDFLNLLTSTDVHYLTQLDWEKINQAEVATGEALGKPR
                     VKIVNREEFLNFKNA"
BASE COUNT          681 a          566 c          575 g          847 t
ORIGIN      
        1 aagtggtttc agtgcatcgg taacttgcat tgccaattca cgagttggaa ctaagaccag
       61 gccaagtgga tgattcttag ttgctttgcg atgagcaagt ttagttagca tcgctaaacc
      121 aaaagcaagc gtttttcctg atccagtttg tccacgaccc aaaacatcac gaccaccaat
      181 tgtgtcgggc aaagttttta cttggattgg gaacggtgtc tctattcctt ggcgttttaa
      241 aaccggaagt aaattttcgt gtacgccaag ctctgaccaa gcgggcaaat caactggttc
      301 aagtttttcg tattcttttt ctttaaccgg tttaataact gccgattttc ttgtttcacg
      361 aatatcggtt cgttcaaatt tttcagttct tttcgggcgc gaattttcct gagtagcttt
      421 ctggcggatt ggccgatctt gaactgcttt aactctggat ggtttctccc gttcactact
      481 cttatattca cttcgttcat gacttggttt ttcacgagcc ggcttttctc ggcttggttt
      541 ttcaaatcga tcttctcttg aatatcggct tgcagatttt ggacgcctag tttcttctct
      601 tgtttcacgt gcttcgcgag cagaacggta agagttttta tttgtggatt ttcccgaagg
      661 gttccaaact tcttttgttt tagttttctt gcggtcagat tttggtgctg aagatttggc
      721 gatagttgca acttcgcggt cagagcgaac tttaactttt ttcgcaggtt ttttgcgctt
      781 atcggatgct gccttctttg cagcttcatg ccgagtagag gtttttttgc cctcagtttt
      841 attgcgcaca cgatctcgct cgagttttcg agcgcgcggt tttttcttag atgttgttga
      901 ggaagtcatt aaaccctttc ggtatctcta cgttaggcga gaaacaccta attacctaat
      961 ctggcaacgc atttttgtca gtaagcaatg cgaaactagt gcaatggatg ctttggtaat
     1021 tgtcggaatt gcagtcgtca gcgtagttat tggattactc gctggccgtt tattcgctaa
     1081 gagtgtaagt ggttttgcgc aattatctgc tgacgagatc cgtcgtcaag ttggtgatgc
     1141 tgcacgtgaa cagttgcagg caatcgcgca gtcgttagag acttcgacag aacaaaggct
     1201 gcaaaccact acgcagtcaa ttgctgagca gaatcgaatg gctagtactt cgatggaagc
     1261 catgattaag cccttgcaag aaacattgaa gtcacttgat acaaaaatta ttgatctgga
     1321 aaatagacgc atcgaggcat acacaagtgt gcaggagcaa gtaaagacaa ctaacgcagc
     1381 actagaggca ctgcgcagtg aaaccacaat gctctctggc gccctgcgcc gcagtgatac
     1441 ccgtggtcgt tggggtgaac ttcaattgcg tcgaattatc gaaatgatga atatgtctga
     1501 gcacatttca tttagcgaac aaatccagca acaaggtgaa ggttcaggaa aacctgatgt
     1561 gacggtttat ctaccgggta atcgagttct ttatctagac agtaaagcac caatgtcgtc
     1621 atacttagat gcaatcaacg agactgacca cattcgcaaa agtgaactac ttgattctca
     1681 cgctaaggca cttaaagccc acgttaaaac actttcaaat cgtgactatg caaaagatga
     1741 agcagcactt aactacgtaa ttatgtttat tccgactgaa tcgtcgttgg cggccgcctg
     1801 tgaaataaac ccaggcttaa ttgaagatgc agcggccagt ggcgtggttt taacttcgcc
     1861 aacttcgcta gttgcggtgc tgagtaatat ttcgatgctg tggcagcagg atgcccaaaa
     1921 tcgaaatgcg gatgaaattc tgcgctcttc acaagaatta cattctcggc tttcagtatt
     1981 tattaaccac ttcaccaaaa ttggtagcaa cctcaaaaaa tccgtggagt cattcaacga
     2041 tgcggttagt tcctttgata cgcgcataat gaaaacggct actgacattc aaaaattagg
     2101 ccgttacaca gatgagctac ctagcgtgcc caaacttgaa gttgaaccaa aaaattcaaa
     2161 gtatgagcag cctgaacttg aaaactaaat ttaagcaagt tttgccattt atttcagctt
     2221 cactttttgg gctttttatt ttttcgctgg gctctgatgt ttgggaagcc tatgcgtttc
     2281 ttttcggcct tagtctgctc gtaaatttat ggattaggcg ttcttaaaat ttaggaactc
     2341 ttctcggtta acaatcttta ctctgggctt tcctagcgct tcgcctgttg caacttcagc
     2401 ttgattgatc ttttcccagt caagctgtgt taagtagtga acatcagttg aagtaagtaa
     2461 gtttagaaaa tcatcagatt cttttggtgc ttttagttca tcaactaaaa acttcatgac
     2521 ttcagtggaa tcgcttttgt tagtgccaat cacacctgaa gggccgcgtt ttgcccagcc
     2581 aactacatat aaattgtcat tcacatggcc gttttcattt tttactcgac ctttttcaaa
     2641 ttgaacatct tcaaattcat tagtctcat
//
LOCUS       DBNHVN010000074         1870 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1648342, whole genome shotgun sequence.
ACCESSION   DBNHVN010000074 DBNHVN010000000
VERSION     DBNHVN010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1648342"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="AC16Y1_01310"
     CDS             <1..393
                     /locus_tag="AC16Y1_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR5831135.1"
                     /translation="VVDGVLVSGTALVSVEGECSRCLEPIAFDMEVDLQELFEYPPTD
                     ARGRQIEKSQTDDELPKLDGDLLDLESVLRDAVVLAFPLSPLCNPGCRGLCSECGANL
                     NENPDHQHETVDPRWSALSKLSTENLPE"
     gene            456..629
                     /gene="rpmF"
                     /locus_tag="AC16Y1_01315"
     CDS             456..629
                     /gene="rpmF"
                     /locus_tag="AC16Y1_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006139588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR5831136.1"
                     /translation="MAVPKRKTSRSNTRHRRSAWKTTAANLATCDRCRAPKLQHTACP
                     TCGTYDKRQVLSV"
     gene            635..1348
                     /gene="rnc"
                     /locus_tag="AC16Y1_01320"
     CDS             635..1348
                     /gene="rnc"
                     /locus_tag="AC16Y1_01320"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR5831137.1"
                     /translation="MAPTATYQSLIDQLELEVNLQLLELALTHRSFAYESGGIPTNER
                     LEFLGDAVLGLVITDELYHRYPDLPEGQLAKFRSAVVNARALAELARSLSIGSYLRLG
                     RGEEATGGRDKSSILADTFEAILGAAYLDHGIEKTRSLILRLFESLLEQAAKLGAGLD
                     WKTSLQELTAELGIGVPSYAVSEKGPDHAKAFTAQVVLGEKLYGHGKGSNKKEAEQQA
                     AASAYAELSVEHEIEEQRE"
     gene            1348..>1870
                     /locus_tag="AC16Y1_01325"
     CDS             1348..>1870
                     /locus_tag="AC16Y1_01325"
                     /inference="COORDINATES: protein motif:HMM:NF013328.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HIR5831138.1"
                     /translation="MPELPEVEVVRRGLAKWVTNKKIETVTVLHPRAIRNFPGTKKSF
                     SNYLVGQKITAVNRRGKYMWLELNSGQSVVTHLGMSGQVLIQKPSQEPEKHLRIRFTF
                     SNYKNEMHFVDQRTFGGMHVDELVRTTANELIPSTVAHIARDPFDPAFNLDEAVQRLR
                     TKRTEVKRALLDQT"
BASE COUNT          557 a          397 c          445 g          471 t
ORIGIN      
        1 gtggtggatg gagttctcgt tagcggtact gcattggtat ctgttgaagg agaatgttca
       61 agatgtcttg agccgattgc tttcgatatg gaagtagatc tacaagaact ttttgaatat
      121 ccgccaacag atgcgcgagg tagacagatt gaaaaatcgc aaactgacga tgaattacca
      181 aagctagatg gagacttact cgatctagaa tcagtactgc gcgacgcagt cgtgttagcg
      241 tttccgcttt cgccattgtg taatccgggt tgtcgtggac tttgctctga atgcggagca
      301 aatttaaacg agaatccaga tcaccaacac gaaacagttg atccgcgatg gtctgcacta
      361 agcaaactct caactgagaa tttgccagaa taaaagtaat aaaagtttga cccgttcgat
      421 aactatcgaa cctgattaga aaaggatggc agaccgtggc cgttccaaaa cgaaaaactt
      481 cacgcagcaa cacccgtcat cgtcgttccg catggaagac aactgctgct aacttggcga
      541 cctgtgatcg ttgccgtgca cctaagttgc agcacactgc ttgcccaact tgtggtactt
      601 acgacaagcg acaggttctt agcgtttaac tactgtggcg cctaccgcta cgtaccaatc
      661 gttaatagat cagctcgaac tagaagttaa tctccagtta cttgaactgg cattaacgca
      721 tagatcattc gcctacgagt caggcggaat tccaacaaac gagcgcctag agtttcttgg
      781 cgatgctgtt ttaggtttag taattacaga tgagctgtac caccggtatc cagatttacc
      841 agaaggtcaa ttagcaaaat ttaggtcagc cgtagttaac gccagggcac tggctgaatt
      901 agcacgcagt ttaagtatcg gatcttattt gcgccttggt cgcggggaag aagcaactgg
      961 cgggcgcgat aagtcttcga tacttgccga tacttttgaa gcaattctgg gtgctgcata
     1021 tttagaccat ggaattgaaa aaactagaag tttgattttg cgattgtttg agtcattgct
     1081 tgaacaagct gcaaaattag gcgctggttt agattggaaa acatcgctgc aagagttaac
     1141 tgcagaactc ggcataggcg taccaagtta tgcagtatct gaaaaaggtc cagaccatgc
     1201 taaagctttt accgcccagg ttgttctagg cgaaaaacta tatggccacg gtaaagggtc
     1261 aaataaaaag gaagctgaac aacaggcggc ggcgagtgcc tacgctgaac tgtccgttga
     1321 gcatgaaatt gaagaacagc gcgaataatg cctgaattac ccgaagtcga agttgtccgg
     1381 cgcggcttag ctaaatgggt tactaacaaa aaaatcgaaa ctgtaactgt cctgcatcca
     1441 agggcaatcc gaaattttcc tggcacgaaa aaaagtttta gtaattactt ggtcggccaa
     1501 aaaataactg cagtcaatcg ccgcggtaaa tacatgtggc ttgagttaaa ttcggggcaa
     1561 tcagtagtga ctcacctggg tatgagtggg caggttctca tccaaaagcc aagtcaagag
     1621 ccagaaaaac acctaagaat tcgattcaca ttcagtaact ataaaaatga gatgcacttt
     1681 gtcgatcaac gcacttttgg tggcatgcat gttgatgaac tagttcggac aactgcgaat
     1741 gaattaattc cgagcacagt tgcccacatt gctcgggatc catttgatcc ggctttcaat
     1801 ctcgacgaag ctgtccaaag attgcggact aaaaggaccg aagtaaaacg agctttatta
     1861 gatcaaactt
//
LOCUS       DBNHVN010000075         1076 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1871302, whole genome shotgun sequence.
ACCESSION   DBNHVN010000075 DBNHVN010000000
VERSION     DBNHVN010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1871302"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /gene="ygfZ"
                     /locus_tag="AC16Y1_01330"
     CDS             <1..688
                     /gene="ygfZ"
                     /locus_tag="AC16Y1_01330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CAF17-like 4Fe-4S cluster assembly/insertion
                     protein YgfZ"
                     /protein_id="HIR5831139.1"
                     /translation="ITWLIVEPETSEQLTKYLESMKFMLRLEVANVSEKFAVVGSPDW
                     IATSYPTWHSPASYVNFDNDPYVTKRPDSWKVSEYIVPRTKLAAEIITPVGSWAWEAH
                     RIRAGVARLNFETDHKTIPHEVGLIGPAVHLNKGCYRGQETIARVVNLGKPPRRLVHL
                     ELDGSTNELPIKDDEVKSGDQVIGRVTSVVQDYESGPVALAVVKRNISPELVLTVNSV
                     SASQTVIVNI"
     gene            complement(703..1074)
                     /locus_tag="AC16Y1_01335"
     CDS             complement(703..1074)
                     /locus_tag="AC16Y1_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015308598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR5831140.1"
                     /translation="MSVEDDISKCAPLADKIWKLRIFEREQLLSRGISVTVETNEVAA
                     ADAGLPILLISQFTLYADTSKGRRPTWLQAARGEQAEPLVNEVARALRDLGAKVETGK
                     FGADMRITQICDGPMTVLVEV"
BASE COUNT          309 a          229 c          231 g          307 t
ORIGIN      
        1 aattacttgg ttaattgttg aacctgagac aagtgagcaa ttaacaaaat atttggagtc
       61 catgaaattt atgttgcggc ttgaagtcgc aaacgtttcc gaaaaatttg ccgttgttgg
      121 atcaccagat tggattgcaa caagttaccc gacgtggcat tccccggcat catacgtaaa
      181 ttttgataat gatccttatg tgactaagcg accggattct tggaaggtct cagagtacat
      241 agtgccgaga accaaattgg cagcagaaat cattacacct gtgggtagtt gggcttggga
      301 agcacatcgt attcgagctg gtgttgcgcg acttaatttt gagactgatc ataaaacaat
      361 cccgcatgaa gttggcttga tcggtcctgc cgttcatttg aataaagggt gttaccgcgg
      421 ccaagaaact atcgctcgag ttgttaattt gggaaaacca ccacgtcgac ttgtgcattt
      481 ggaattagat ggttcgacca acgaattacc aattaaagat gatgaagtta aatcaggcga
      541 tcaagtaatt ggtcgagtta cttcagtcgt tcaagattat gaatcaggtc cagttgcgct
      601 ggctgtggtg aaacgaaata tttcgccaga attagttctt acggttaatt ccgttagcgc
      661 tagccaaacc gtgatcgtca acatttgatt aacttttaaa aatcaaactt caaccaaaac
      721 cgtcattgga ccatcacaaa tctgagtgat tctcatgtca gcgccaaatt ttccagtctc
      781 aactttggca cctaagtcac gcaatgcccg agcaacttcg ttaactaatg gttcagcctg
      841 ctcacctctg gctgcttgta accaggttgg gcgacggcct tttgaagtat cggcatacaa
      901 agtgaactgg ctaattaaaa gaattggcaa cccagcatca gccgcagcaa cttcatttgt
      961 ttcaactgtt acagaaatac ctcgactgag tagttgctct cgctcaaaga ttcttaactt
     1021 ccaaattttg tcagctaaag gtgcgcattt tgaaatgtca tcctcaacac tgattc
//
LOCUS       DBNHVN010000076         5192 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1871735, whole genome shotgun sequence.
ACCESSION   DBNHVN010000076 DBNHVN010000000
VERSION     DBNHVN010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5192)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1871735"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="AC16Y1_01340"
     CDS             <1..1202
                     /locus_tag="AC16Y1_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HIR5831141.1"
                     /translation="DLVEQVRHRIRLGNSNVLIKEVELKHTLEDVIAEFNPNQLNTDL
                     TSPAIGVKEEAIQELSGLGKLQPLLDDSTVEEIWINEPGRVFVARSGQSELTNLILTD
                     QQVRTLVERMLASTGRRVDLANPFVDATLPDGSRLHVVIPDITKNHWSVNIRKFVMGS
                     KSLNALVEIGTLPANAANFLHASVASGLNIIIAGATQAGKTTLMNALLNSARSGDRII
                     TCEEVFELQLTSPDWVALQTRQESLEGTGEVTLRRLIKESLRMRPSRLVVGEVRQEEC
                     LDLLVAMNSGMPAMCSVHANSARQAISKLCLLPMLAGSNVSAEFVVPTVASVVDVVVH
                     TALNKNGSRRIQEIISVTGRVESGSVETVEIFTNSQDQLLPTGLYPAKLEKFISAGFN
                     LAEILGN"
     gene            1204..1881
                     /locus_tag="AC16Y1_01345"
     CDS             1204..1881
                     /locus_tag="AC16Y1_01345"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR5831142.1"
                     /translation="MSIILGFCYALAVLSLFSYLKYPESTFNLVKKFVFISSPKKSKI
                     QTDLWPDVVDDLASSIRAGLSLPQAMISLSQSGPDLIRPKLAVAMNRYKLTGDFIGSL
                     SLLRVAMKDPVVDKFTNALEIAYEVGGTDLGQLLRSLSEVIREDNKIRGEINARQSWT
                     INGARLAIAAPWLTVLILSTRQSAANIYLSSGGIRLLLFCALVSILAYLAMVRLGQLP
                     TLKRISP"
     gene            1878..2768
                     /locus_tag="AC16Y1_01350"
     CDS             1878..2768
                     /locus_tag="AC16Y1_01350"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR5831143.1"
                     /translation="MKLTSFLIGGLGASGLLLIFRSLLVRRISMAESIAPYLGLPVEG
                     KNIGLALIGKIRNSLIKGNSAPWASDTRVLHELRRCSSSQSLASLRFEQLIFASLATL
                     FAACWISLRIFAGHEVSAFIGITLLFAAFLFGGSFRSWLLKETGRKRIKEIETQLPTV
                     LDLLAFSVSAGEPILSSMQRVAKTTSGELATEIKRLTTGLALGENFLSGLERIQKELG
                     SQSVARAFRALIMAMERGTPIADVLRAQAMDARQLEARKLMVLAGKKETFMMIPVVFL
                     ILPMIVIVALYPGLMALQTF"
     gene            2780..2998
                     /locus_tag="AC16Y1_01355"
     CDS             2780..2998
                     /locus_tag="AC16Y1_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831144.1"
                     /translation="MKKIDKINQWLAKLFAEGEFRLIGIGKNDRGDVPGWVLVTVMTA
                     GLVVALWSIADGQLRSVLQNAIRSVSSP"
     gene            2970..3359
                     /locus_tag="AC16Y1_01360"
     CDS             2970..3359
                     /locus_tag="AC16Y1_01360"
                     /inference="COORDINATES: protein motif:HMM:NF019428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HIR5831145.1"
                     /translation="MRFGQFLLRDRASNTVGFALVAPILVASFLALAQIANLANVQVT
                     LATAARSAAREGSRYDGSIADAVRQANKVLSAQGIQDGTLVQVESTSLSGNQVTQVKI
                     TKKYRISWLNYDLVLISTGQSVDEKNL"
     gene            3343..3759
                     /locus_tag="AC16Y1_01365"
     CDS             3343..3759
                     /locus_tag="AC16Y1_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HIR5831146.1"
                     /translation="MKKIFEDFSSELGNVVIEFTLTAVALFVPIAYISVATTQVALSY
                     IEVQDAARAGARLFATSEIEASGRAASLALAISMLSNSNDVEIAISCSKNPCLARDGI
                     VSFQINKIINLSLPLLPYPISITVSGAQAEVVQDSE"
     gene            3756..4196
                     /locus_tag="AC16Y1_01370"
     CDS             3756..4196
                     /locus_tag="AC16Y1_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HIR5831147.1"
                     /translation="MKLRPKESECGSVLVFGVGLGIVVLLILTTSINVASLWVTRTKL
                     DSVADAVALAASRSIDVENLYRAGISQPIKLSKSIARSRASWYLDQLALQTQLTNFQL
                     VNLSVADNSVEVVISADADIPFGYLTYGLDSTVFSRAKVSIKTK"
     gene            4232..>5192
                     /gene="prfB"
                     /locus_tag="AC16Y1_01375"
     CDS             4232..>5192
                     /gene="prfB"
                     /locus_tag="AC16Y1_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR5831148.1"
                     /translation="MGTGDFAADFAILNSTFRNIERVLNPDAMATEVAELEKEASAPD
                     LWDDQAKAQQVTSRLSALQSDLARLRNLRSRLDDVQVLRELGEAENDQSILTESANEL
                     AAIESIISELEVRTLLSGEYDSREALVSIRSGAGGVDAADWAEMLLRMYQRYCERHNW
                     STEIYDTSYAEEAGIKSATFAVHAPYAYGTLSVEQGTHRLVRISPFDSQSRRHTSFAE
                     VEVIPVVEQSDSIDIPEDQLRIDVYRSSGPGGQGVNTTDSAVRITHIPTGIVVSCQNE
                     RSQLQNKATAMAVLQAKLLARKREEERAKIDALKGDNLGSWGNQ"
BASE COUNT         1491 a         1017 c         1202 g         1482 t
ORIGIN      
        1 cagatttagt tgagcaggtt cggcatcgaa ttcgattagg aaactcaaat gttttgataa
       61 aagaagtgga actaaagcac acgcttgaag atgtaattgc cgaatttaat ccaaatcaat
      121 tgaacacaga cttaacttcg ccagctattg gagtaaaaga agaggctatt caagaactct
      181 ctgggttggg aaaattacag cccttgctcg acgattcaac ggttgaagaa atctggatta
      241 atgaaccggg tcgagtgttt gttgccagaa gtggacaaag tgagctaaca aatctgatcc
      301 tcactgatca gcaggttaga actcttgttg aacggatgtt ggcctcaact ggccgacggg
      361 ttgatttagc aaatccattt gtagatgcaa cgttgcccga tggttcgcgg ttacatgtgg
      421 tgattccaga catcacaaaa aaccactggt cggtgaatat ccgtaaattt gttatgggat
      481 caaagtcact gaacgcttta gttgaaattg gaactttacc tgcaaatgcc gcaaattttt
      541 tacacgcctc agtagcctcc ggcctaaaca tcatcattgc cggtgcaact caagctggaa
      601 aaacaacctt aatgaatgca cttttgaata gcgcacgctc tggtgatcgg ataattacct
      661 gcgaagaagt ttttgagttg cagctcacga gccctgactg ggtagctttg caaacaagac
      721 aagagagcct agaaggaaca ggtgaagtca cgcttagacg tttaattaaa gaatcactaa
      781 gaatgcgtcc aagtcgcttg gtagtcggcg aagtccgaca agaagaatgc ctcgacttat
      841 tggttgcgat gaattctggg atgcctgcaa tgtgttcagt tcatgcaaac agcgcaagac
      901 aagcaatttc taagttatgc ctactgccta tgttggcggg tagcaatgtc tctgcggaat
      961 tcgtggtacc aactgtggct tcggtcgtcg atgtagttgt tcatactgca ttgaataaaa
     1021 atggcagtag gagaattcaa gaaataattt ccgtcactgg acgcgttgaa tccggaagtg
     1081 tcgaaacggt tgagattttt acaaattcgc aagatcaatt actacccact ggtttgtacc
     1141 ctgcaaagtt agaaaaattt atcagcgctg gatttaattt agctgaaatt cttggaaatt
     1201 aatatgagta ttattttagg tttttgttat gcacttgcgg tactttcctt gtttagttat
     1261 ttaaaatatc cggaatcaac ctttaattta gtcaaaaagt ttgtttttat ttcctcacca
     1321 aaaaaatcaa aaatacaaac tgatttgtgg ccagatgttg tcgatgatct agcgtcctca
     1381 attcgggctg ggctatcttt acctcaagcg atgattagct tgtcgcagtc gggtcctgat
     1441 ctaattcgac ctaagttagc agttgcaatg aaccggtata agctcaccgg agatttcatt
     1501 ggttccttaa gtcttttaag agttgccatg aaggatccag ttgtggataa atttacgaac
     1561 gctctagaaa tcgcatatga agtcggtgga acggacttag ggcaactact tagaagcctg
     1621 agtgaagtaa tccgagaaga caataaaata cggggagaaa taaatgcccg gcaaagctgg
     1681 acaattaacg gggcgagact ggccattgcc gctccttggt tgactgtatt aatacttagc
     1741 accagacagt cagcggcaaa tatttactta tccagcggtg gtattagatt gctacttttt
     1801 tgtgcacttg tatcgatact tgcctaccta gccatggtca ggttgggtca attaccaaca
     1861 ttaaagcgaa tcagcccatg aagttaactt cttttctgat cggtgggtta ggtgcatctg
     1921 gcttacttct gatttttcgc agcctcctag ttagacgaat ttcgatggca gagtccattg
     1981 cgccatatct tggattacct gttgaaggca aaaacatagg cttagcactc attggaaaaa
     2041 taagaaactc attgattaag ggaaactcag caccttgggc gagtgatacc cgggttctcc
     2101 atgagttaag aaggtgttca agttctcaat cactagcaag tttgcgattc gaacaactaa
     2161 tttttgcttc gctagctacg ttgtttgcag cttgctggat atcacttcgt atatttgctg
     2221 gccatgaagt aagcgctttt attggtatta ccttgttatt tgcagcgttc cttttcggtg
     2281 gatctttcag gagttggttg ctcaaggaaa caggaagaaa aagaattaaa gaaatagaga
     2341 ctcaattacc tactgtgtta gatctcttgg cattttctgt ttcagctggt gaaccaattt
     2401 tgtcttcaat gcaaagagtt gcaaaaacaa cttcaggcga gttggcaacc gaaatcaaac
     2461 gattaacaac tggtctcgct ctgggtgaaa actttttatc aggacttgag cggattcaga
     2521 aagaacttgg aagccaatca gttgcgcgag ctttccgtgc attgattatg gcgatggagc
     2581 gaggtacgcc tatcgcagat gtattacgag cccaagcgat ggatgcgcgg caactagaag
     2641 ctaggaaatt aatggttctt gcgggtaaaa aagaaacttt catgatgatc ccagttgtgt
     2701 ttttaattct gccgatgatt gtgattgttg ctttatatcc gggattaatg gcacttcaaa
     2761 ctttctaaaa ggaggaaaaa tgaaaaaaat agacaagata aatcaatggt tagcgaagct
     2821 ttttgcagaa ggagaatttc gcttaatcgg tatcggaaaa aatgacaggg gcgatgttcc
     2881 aggttgggta ttagttaccg tcatgacagc aggattagtg gtagcccttt ggtcaattgc
     2941 agatggtcaa ctgcgatcgg tattgcaaaa tgcgattcgg tcagtttctt ctccgtgaca
     3001 gagcctcgaa taccgttggc tttgcacttg ttgcgccaat tttggttgct agttttttag
     3061 cgctcgctca gatcgccaat ctggctaacg ttcaagtaac tttggcaaca gccgcaagat
     3121 ccgcagcacg tgaaggtagt cgttacgacg gttcgatagc tgatgcagta cgccaggcaa
     3181 ataaagtttt atcagcgcaa ggcatccaag atggaacttt agtacaagtt gagagtactt
     3241 cgcttagtgg caatcaagtt acccaagtaa agataactaa aaaatatcgc atctcatggc
     3301 tgaactatga cttagtactg atttcaacag gacaatccgt agatgaaaaa aatctttgaa
     3361 gatttttcaa gcgagctggg aaatgtcgtt attgaattca ctttgactgc agttgctctt
     3421 ttcgtgccga tagcctacat ttcagttgca acaacccagg ttgcacttag ttatattgaa
     3481 gttcaagatg ccgctcgggc tggggcaaga ttatttgcta ccagtgaaat tgaggccagt
     3541 ggtcgtgctg cgagcttggc gttagctatt tcgatgcttt ctaattcgaa cgatgtggag
     3601 attgcaatta gttgcagtaa aaatccttgc cttgctcgtg atggaatagt cagttttcag
     3661 attaataaaa taataaattt atccctgcct ttacttccgt acccaatatc aattaccgtt
     3721 tctggtgctc aggcagaagt cgtacaggac tcagagtgaa attgagaccc aaagagtcgg
     3781 aatgtggctc cgttttagtt tttggagtag gtcttggaat cgttgtgctg ttgattctta
     3841 caacttctat aaatgtagcc tcactatggg taaccagaac aaaattagac agtgttgctg
     3901 atgctgtcgc attagcagcg agccgatcga tagatgtcga aaacttgtat cgagcaggga
     3961 tttcacaacc aattaaatta agcaaatcaa tcgccagatc tcgagcatct tggtacttag
     4021 accaacttgc ccttcaaact caattaacta actttcaact tgtgaatctt tcagttgccg
     4081 ataactctgt tgaagtagtt attagcgcgg acgcagatat tccatttgga tatttaacct
     4141 atgggttgga ttcaacggtt ttttcgcgag ccaaagtttc cataaaaacc aagtaattat
     4201 ttctttttaa aacacggtaa tcttaagcat tgtgggaact ggcgactttg cagcagactt
     4261 tgcgattcta aattccactt ttaggaatat tgaaagagtt ctaaatccag atgccatggc
     4321 caccgaagtg gctgaactcg agaaagaagc ttctgcgcca gatctttggg atgaccaagc
     4381 aaaagcacaa caagtaacca gccgactatc agctttgcag tcagacttag ccagattgcg
     4441 aaatttaaga agtcgccttg atgacgttca ggtacttcgc gaactgggtg aggctgaaaa
     4501 tgatcagtca attctgactg agtcagcaaa tgaattagcc gctattgaat caatcataag
     4561 cgaattggaa gttcgaactt tgctctccgg tgagtatgac tcgcgagagg cgctggtctc
     4621 tattcgatca ggtgcaggcg gagtggatgc ggctgactgg gcagaaatgc ttctgcggat
     4681 gtatcagcgt tattgcgaac gccacaattg gtcaacggaa atttacgaca cgtcgtatgc
     4741 agaagaagcc ggaatcaagt cagcaacttt tgccgttcat gctccgtatg cctacggcac
     4801 actttctgtt gagcaaggca cgcatcgctt ggttcgaatt tcaccttttg atagccagag
     4861 tcgccgccat acgtcctttg ctgaagtaga agtaattccc gttgtagaac aaagtgactc
     4921 aatcgatatt cctgaggatc aattacgaat agatgtgtac cgttcgtctg gacctggtgg
     4981 ccaaggtgtt aacacaacag actctgcagt tcgaattact cacataccga ctggcattgt
     5041 ggtgtcttgt cagaatgaac gttcgcaatt acaaaataaa gctacggcaa tggcagtttt
     5101 acaagcaaag ttattggcca gaaaacgtga agaagaacga gcaaaaatag atgcacttaa
     5161 aggtgacaac ttaggttctt ggggtaatca ga
//
LOCUS       DBNHVN010000077         3650 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1277290, whole genome shotgun sequence.
ACCESSION   DBNHVN010000077 DBNHVN010000000
VERSION     DBNHVN010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1277290"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="AC16Y1_01380"
     CDS             complement(<1..1253)
                     /locus_tag="AC16Y1_01380"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HIR5831149.1"
                     /translation="MSELVGILIFAFGILFSIGLHEIGHLLPAKKFGVRVPTYMVGFG
                     PTIWSKVKGETEYGVKLLPFGGYIRMIGMFPPSQKEIEDPSKLGRMGSLIEQARQETL
                     SEIADVDQNRVFYKLSVPKKLTVMFGGPFMNLIIATVLFFVTLSGVGISAPSTTLSNV
                     TACVPSADNPSGIASTDGSCGDGVKGAAYAAGITAGDKLISIDGIVIQSWDDVSGALA
                     GKVGMVVPVVVERAGSEVTLRPTVGEIEDTPGAPRAFLGISPSTYMERMPASEVPAEM
                     WNFTVLSVQGLLSFPAEVLNLGQELFTDTPRDIEGPVSVIGVGRISGQVTGADAISNL
                     DKLSILLMLLASLNLFLFLFNLVPVLPLDGGHIAGALYEGARKSIAKIFGRPNPGPVD
                     TAKMLPVAYVATLILLSMALVVMLA"
     gene            complement(1302..2504)
                     /gene="dxr"
                     /locus_tag="AC16Y1_01385"
     CDS             complement(1302..2504)
                     /gene="dxr"
                     /locus_tag="AC16Y1_01385"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR5831150.1"
                     /translation="MQRRITILGATGSIGTQALDIVRLNPNEFKVVGLSAGGNNVALL
                     AKLALEFKVEQVAIANGTAAQDFQLAIYAQASKEGYAEGSFEVPKLIIGPNAASELAA
                     VSVDVVLNGITGAVGLGPTVAALKAGTQLALANKESLVIGGQAVIRLAKPGQIVPVDS
                     EHSAIAQCLRSGSTSEVNKLILTASGGPFRGFTKAQLEEVSIEQALAHPTWSMGPVVT
                     INSSTMMNKGLEIIEAHLLFEIPMERIEVVVHPQSVVHSMVEFVDGSTIAQASPPDMH
                     LPIALGLTWPNRLANSSIPCDWTSKSEWTFEPVDHDVFPAISLAKRAGLTAGSAPAVL
                     NGANEVAVAAFLAGELKYSQIVPTVSQIVESHLATDFVSDDRLTIEEVVKAADWAQNA
                     CTTLISNT"
     gene            complement(2523..>3650)
                     /locus_tag="AC16Y1_01390"
     CDS             complement(2523..>3650)
                     /locus_tag="AC16Y1_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5831151.1"
                     /translation="TVFRWAAEEVRRFSGELQRLDTEAAAAGRMAIIRRFPHGPVLGI
                     SPFNFPMNLVAHKVAPAIAVGAPIIVKPAPSTPLSALLLGEILAQADLPEGSWSILPI
                     ANADAPALVADPRLPVVSFTGSDKVGYEIQKAVPHKHITLELGGNAAAIVNEDWNTEA
                     DLNWAATRIATFANYQAGQSCISVQRVLIHENLYDELTKRIVQNVAGLPNGDPRDEKT
                     VVGPLINEAAAIRVEQWVNEAVANGAKVLTGGKRNGSFYEPTVLANVPLDAKVSCNEV
                     FGPVMLVHKITSFDEGIAVVNDSIFGLQTGVFTHDIQKAFHAHRELEVGGVIVGDVPS
                     YRSDQMPYGGIKASGVGKEGLRYAMQDFTHERIMVLSGLDL"
BASE COUNT         1025 a          885 c          741 g          999 t
ORIGIN      
        1 tcggccaaca taaccactaa ggccatggat aacaaaatta acgtagctac gtaagccacc
       61 gggagcatct ttgccgtatc aactgggcct ggatttggcc ggccaaaaat tttggcaatt
      121 gacttacgag caccttcata aagcgctcca gcaatgtgtc caccatcgag aggtaacact
      181 ggaactaagt taaacaaaaa tagaaacaga tttagggaag ctaatagcat tagcaaaatc
      241 gataatttgt ctaaattact gatggcatct gcaccggtaa cttgaccact gattcgccca
      301 acaccaatca cgctaacggg gccttcaata tctctaggtg tatcagtaaa taattcttgg
      361 cctaagttca gaacttccgc tggaaaactt agtaacccct gtactgataa aacggtaaag
      421 ttccacatct ctgctggtac ttcgcttgcc ggcattctct ccatataagt cgatggtgaa
      481 atacccaaga atgcacgtgg cgcacctgga gtatcttcaa tttccccaac agttggcctt
      541 agcgtcactt cacttcctgc acgttcaaca acgaccggaa caaccatgcc aactttgccg
      601 gcaagtgccc cgctcacgtc atcccaagat tgaatgacga ttccatcgat acttatcaat
      661 ttgtcgccag ctgtaattcc cgcagcgtat gcagcgcctt taactccatc tccacaactg
      721 ccgtcagtgg aagcaatacc acttgggttg tcggcagatg gcacacaagc cgtcacatta
      781 ctcagagttg ttgatggtgc agagatgcca acacctgaaa gtgtcacgaa aaataaaacc
      841 gttgcaataa ttaaattcat aaacggtcca ccgaacataa ccgttaattt ttttggcacg
      901 gaaagtttgt agaaaactcg attttgatca acatccgcga tttcacttag tgtttcttga
      961 cgggcctgtt cgatcaaact acccattcgc ccaagtttcg atggatcttc aatttctttt
     1021 tggctcggtg ggaacattcc gatcattcgg atgtatccac caaaaggtaa caacttaact
     1081 ccgtattcgg tttctccttt tactttggac caaattgttg ggccaaaacc aaccatgtaa
     1141 gtcggaacgc gcacaccaaa ttttttggca gggagtaagt gaccaatttc gtgcaatccg
     1201 attgaaaaaa gaattccgaa ggcaaaaatc aaaatgccca ctaattcact cactaaagcc
     1261 ccgccttttt atatgcagta ttaatttgta tgcagtatta atcaagtatt actgattaac
     1321 gtagtgcagg cattttgtgc ccaatctgca gcttttacta cttcttcaat agtaagccga
     1381 tcgtctgaca caaaatcagt tgccaaatgt gattccacta tttgcgaaac tgttggaaca
     1441 atttggctgt acttcaattc accggccaaa aaagccgcga ccgctacttc gttggcacca
     1501 ttcaatacag ctggggcgct acctgcagtg aggccagcac gctttgcaag tgaaattgct
     1561 gggaatacat catgatcaac tggttcaaat gtccattcac tcttcgaagt ccaatcacaa
     1621 gggattgaac tgtttgctaa ccgattcggc caagttaaac ctagtgcgat tggtaaatgc
     1681 atatccggtg ggctcgcttg ggcaattgtt gagccgtcca caaactcgac cattgaatga
     1741 actaccgact gcggatgtac tacaacttca attcgctcca tcgggatttc aaaaagtaaa
     1801 tgtgcctcaa tgatttctaa acctttattc atcatggtgg atgaatttat tgttactact
     1861 ggtcccatcg accaggttgg gtgagccaag gcttgttcga ttgaaacttc ttctagttgt
     1921 gcttttgtga acccgcgaaa cggtccacca cttgccgtca gaattagttt gttaacttca
     1981 ctcgttgaac cagaacgtaa acattgtgcg atcgcactat gttctgaatc aactggaact
     2041 atctgcccag gtttagctag cctgatcaca gcttggccac cgataactag agattcttta
     2101 ttggctaatg caagttgcgt acctgctttt agcgctgcga ctgttggacc gagtccaacg
     2161 gcaccggtga ttccattaag gacaacatca accgaaaccg cggcaagttc gctagctgca
     2221 tttggtccaa taatgagttt tggaacttca aaggagcctt cggcataacc ttctttactt
     2281 gcttgtgcat aaatcgctaa ctgaaaatct tgtgcggccg taccatttgc gatggctacc
     2341 tgttcgacct taaattccag ggccagcttg gccaaaagtg caacattatt tccgccagca
     2401 cttagtccaa caactttgaa ttcgtttggg ttaagtcgaa cgatgtctaa agcttgagta
     2461 ccgatacttc cggtcgcccc aagaattgtt attcgtcgct gcactgttac ccgattaaag
     2521 atttaaaggt caaggccact taacaccatg atgcgctcat gagtgaagtc ttgcatcgca
     2581 taacgcaaac cttctttacc gacaccggat gctttaatgc cgccgtaagg catttgatcc
     2641 gatcgatatg aaggcacatc tccgacaatt acgccaccga cttcaagttc acgatgagca
     2701 tggaatgcct tttgaatgtc atgtgtgaag acaccagttt gtaaaccaaa gattgaatcg
     2761 tttaccacag caattccttc atcaaaagat gtaattttat gaaccaacat gactggacca
     2821 aatacttcgt tgcaagaaac ttttgcatca agcggaacat tagctaaaac agttggttca
     2881 taaaaagagc cattgcgttt tccgccagtt aaaactttgg cgccgttagc cacggcttca
     2941 ttaacccact gctccacacg aatcgcagct gcttcattaa taagtgggcc aacaacagtc
     3001 ttttcatcac gaggatcgcc atttggtaat cccgcaacat tctgcacaat ccgttttgtt
     3061 aattcgtcat ataagttttc atgaattaat acccgttgaa ccgaaataca agattgaccg
     3121 gcttgatagt ttgcaaaagt tgcaatgcgg gttgctgccc agtttagatc tgcctctgta
     3181 ttccaatctt catttactat cgcggcagca ttcccaccaa gttctaatgt gatgtgctta
     3241 tgcggaactg ccttttgaat ttcatagcca actttgtctg aaccagtaaa cgaaacaact
     3301 ggtaagcgtg gatctgcaac tagtgctggt gcatcggcat ttgcaattgg cagtattgac
     3361 caagaacctt ctggtaaatc tgcttgcgcc aaaatttcac caagtagtaa cgcgctaagc
     3421 ggtgtactcg gtgcaggttt cacaattatg ggtgcgccaa cagcaattgc tggagcaact
     3481 ttatgtgcaa ctaaattcat tggaaaatta aatggtgaaa taccaagcac tggaccgtgt
     3541 gggaatcgac gaataattgc cattcgtcca gcggcagcgg cttcagtatc taagcgctga
     3601 agttcgcctg agaaacgacg aacctcttct gccgcccatc taaatacagt
//
LOCUS       DBNHVN010000078         5041 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1351866, whole genome shotgun sequence.
ACCESSION   DBNHVN010000078 DBNHVN010000000
VERSION     DBNHVN010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5041)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1351866"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..635
                     /locus_tag="AC16Y1_01395"
     CDS             132..635
                     /locus_tag="AC16Y1_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5318 family protein"
                     /protein_id="HIR5831152.1"
                     /translation="MAKPAQSIKGYRLAGMSTEPLRPIPHPFEDVADPARPRHVLDYV
                     LARRATLETIKNDALAREQVCDADPYLLRAAKNHGEQTERKCPICAKSDLVHVTYVFG
                     DDLGYLAGRVKNSAELPQMAHEYGHFRVYVVEVCVLCAWNHLQLSYVLGDGNPRRPPS
                     KPRDVLE"
     gene            640..2796
                     /locus_tag="AC16Y1_01400"
     CDS             640..2796
                     /locus_tag="AC16Y1_01400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR5831153.1"
                     /translation="MGKPKKKSKFKLRKYLPTRNQFLIGFFVSGVLGVTFIFAAVRAV
                     EVPAPSDVSQAQATILYYDDGVTELGRLGESNRVSLPLLEIPVLTQQAVLAAEDRDFY
                     EHGGFSVRGIARAFFSNATSNTTAGGSTITQQYAKNAYLSQEQTIKRKVREVILSVKL
                     ETMITKEKILENYLNTIYFGRKSYGIETGANAYFGKAAKDLTISESAFLASIIQAPNG
                     LAPEKNIEGLMARWNYTLDGMVEKGWITQEQRVLQVFPSFLEYTNANSFAGPVGHLVE
                     QSRQELFRLGITEDQINRAGLRVTTTFNKQAQDAAITAVIEEGPTEGTEGLRIGLAAV
                     RPGTGEVVAIYGGDDYLEDQFNNATQAIGQGGSTFKPFTLAAALENDFSLGSVFSGMN
                     KTKVGTYEVVNYGSNSYGEWITLLLATENSVNSAYVQLADQVGLETVYQAAIRAGIPA
                     GEVGMMPNLAFTLGTASPNVVDIANGYSTFAARGLQTDLSYIKVIAAKNGDVLYEYVP
                     QPVQAFSTDVADTVSYALKKVVESGTGRAALRVGRPVAGKTGTTNDNKSALFAGYTPD
                     LAVAVMFTKDGPNGESVSLSGTGGMRTVTGGSFPARIFTAFMRGALEGIPAKQFGALP
                     ANAPNGATPTGTPIYTGIDPALVGQQVTVPSLIIPLADARAVAAANGVVLVESYAANV
                     DQNATLYVINQSVPAGTVVMAGTIVGVTISNSAAAP"
     gene            complement(2817..3965)
                     /locus_tag="AC16Y1_01405"
     CDS             complement(2817..3965)
                     /locus_tag="AC16Y1_01405"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR5831154.1"
                     /translation="MTFTLTIDRKIFNKHLASTLEQFEKSGATVTPVIKGNGYGLGRR
                     LLAKESTVLGLKRIAVGTIYELDQALTDFGDEIVVLEPFNPHDEPAIAAWERLMPLGA
                     HRVIATLSGPDFAQASRVGIKKAFIEGRTSLHRFGISAAELVSIANGDHHNIEILGLS
                     LHSPISDPVNVLLPTLEGSAKLNSKKSTSRIIEIASWLSSFASVTEKMNWPMHISLSH
                     VSAKDIREIQEITKERNLNISLEVRLGTHLWLGELKALEITGTVLEIHELTGDHEHVG
                     YRQVDAHGNARLLVVSGGTAHGVALAAPTNRSTLRGKSVAVAEGIAQAIGKVRSPFKL
                     NGKNLPFAEPPHMHVSLLWTDDKKVKVGDQLNCTVRNTTATFDQIIDK"
     gene            complement(3970..>5041)
                     /locus_tag="AC16Y1_01410"
     CDS             complement(3970..>5041)
                     /locus_tag="AC16Y1_01410"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003953075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HIR5831155.1"
                     /translation="NSRPSVSFLQTPAWGETKVGWQRKSLGWFEDGQLIGAGLVLLRK
                     VPKLNKYLAYLPEGPDLDWAQQDLSGALNALIEYLKPFGVFQIKMGPHVWVRRWSAQT
                     LKDAIANESAKRISELNPDETNHVGINLVTQLKHLGWKQPKADQASFGDYQPRFVFQV
                     SLKNKTEEDLLNSFNQQWRRNIKKAQNDGVTVRLGNETDLELFHTCYIETAKRDRFTP
                     RPLSYFRTMWQALSSEDPGRIKLFIAEHKDHQGAIAATTLTRVGNHSWYSYGASTTSA
                     RDLRPSNAIQWEMMKDSLHSGSEIYDMRGIADTLDQNNHLFGLIQFKLGTSGYAQEYV
                     GEWDYVISKSWSTAFNLYMSRR"
BASE COUNT         1450 a         1080 c         1073 g         1438 t
ORIGIN      
        1 agcttcagcc cgctttgcca taattaatct ttcaatcaga tgtatcaaaa agatacatca
       61 tgaggataca tcatattcag cgggggattt aaaaggaaaa acccatgatt taaggcagtt
      121 aggcttatct agtggcaaaa ccagcgcaaa gtatcaaggg ctaccgcctt gccggtatga
      181 gcacagagcc attacggcca atcccccatc cgtttgagga tgtggctgac ccggctagac
      241 ccaggcatgt gctcgattac gttttagctc gtcgagccac gttggaaact atcaagaatg
      301 atgcgctggc tagagagcaa gtatgtgatg ctgatccata tttattgcgg gcagcaaaaa
      361 atcatggtga acaaactgag cgcaaatgcc caatttgtgc caaatccgac ttagttcacg
      421 ttacttatgt attcggcgac gatcttggat atttagctgg tcgagtaaaa aattccgccg
      481 aattgccgca aatggcacat gagtacggcc actttagggt ttatgttgtt gaagtctgtg
      541 ttttatgtgc ttggaatcac ttacaacttt cctatgtact tggggacggt aacccacgcc
      601 gaccacctag caaacctcga gatgtgttgg aatagataaa tgggtaaacc gaagaaaaag
      661 tcgaagttca aattgcgcaa gtatttgcca actcgaaatc aatttttaat cggctttttt
      721 gtatctggcg tacttggcgt aacttttatt tttgccgcag ttcgcgcagt tgaagttcct
      781 gcaccgagtg acgtatcgca agctcaagcg acaattcttt attacgacga tggggtaacc
      841 gaactcggtc gactaggtga atcaaatcgt gtctcacttc cgttattaga aattccggta
      901 cttacccagc aagcagtttt ggctgctgag gatagagatt tctatgagca tggtggtttt
      961 tctgttcgcg gcatcgcgag agcatttttt agtaatgcaa cttcgaatac cacagctggt
     1021 ggttcaacaa ttacccagca atatgcaaaa aatgcttacc tctcacaaga acaaacgatt
     1081 aaacgtaaag ttcgtgaagt aatcctttcg gtgaaactcg aaacgatgat tacaaaagaa
     1141 aaaatccttg aaaattattt gaacacaatc tactttggcc ggaaatcata cggaatcgaa
     1201 actggcgcaa atgcttactt cggtaaagcc gcaaaagatt taacaatttc tgaatcagca
     1261 tttttagctt ctatcattca ggcacccaac gggttagctc cagaaaaaaa catcgaaggc
     1321 ctgatggcac ggtggaatta cacacttgat ggcatggtag aaaaaggctg gattactcaa
     1381 gagcaaagag tgttacaagt ttttccaagt ttccttgaat acacgaacgc aaattcattt
     1441 gctggcccag tcggacattt agttgaacaa tctcgacaag aactttttcg acttggaatt
     1501 accgaagatc aaattaaccg agcaggttta cgagtaacca caacttttaa taagcaagca
     1561 caagacgcag caatcactgc agtgattgaa gaagggccaa ccgaaggtac tgaaggttta
     1621 agaattggtt tagctgcagt tcgtccaggc actggcgaag tggtagcaat ttatggcggt
     1681 gacgactatt tagaagatca atttaataat gcgacacaag caataggcca aggtggttcg
     1741 actttcaaac cttttacttt ggcagcagcg ttagaaaatg atttttcgct cggcagcgta
     1801 tttagcggaa tgaataaaac aaaagttggt acttacgaag ttgttaacta cggcagcaat
     1861 tcctacggcg aatggataac acttttacta gctactgaaa attcagttaa ctcggcttac
     1921 gttcaactgg cagatcaggt tgggttagaa accgtatatc aagcagctat tcgtgctgga
     1981 attccagcgg gtgaagttgg catgatgcca aacttggcgt ttactttagg tactgcaagc
     2041 ccaaatgttg tggacattgc caacggctat tcaacatttg ctgcccgtgg gttacaaact
     2101 gacttgtctt acataaaagt aatcgcggct aaaaatggcg atgtacttta cgagtatgtt
     2161 ccacaaccag tacaagcgtt cagtactgat gtagccgaca cagttagtta cgcgcttaag
     2221 aaagtagttg aatctggtac tggtcgagct gccctaagag taggtcgccc agttgcaggt
     2281 aaaacgggta caaccaacga caataagtcg gcactttttg ctggctacac tcctgattta
     2341 gcagttgcgg ttatgttcac taaagatggg ccaaatggcg aatcagtttc attaagcgga
     2401 accggcggaa tgcgaactgt taccggtggt tcattcccag ctcgtatttt tacagcattt
     2461 atgcgtgggg cacttgaggg aattccggca aaacaattcg gtgcgttacc agcgaatgca
     2521 cctaatggag caactccaac aggtacaccg atttacaccg gaatagaccc agcactagtt
     2581 ggtcaacaag tcacggttcc ttcactaatc attccgctgg ccgatgctcg tgcagttgct
     2641 gcagctaacg gtgtcgtttt ggttgaaagc tatgcagcga atgtcgatca gaatgcaact
     2701 ttgtacgtaa tcaatcaatc ggtaccggcc ggaacagttg taatggctgg aacaattgtt
     2761 ggtgtgacga ttagtaatag tgcagcagca ccgtaattta aaaattattt tcagtcttat
     2821 ttgtcaataa tttgatcgaa agttgcagtg gtattgcgca cagtgcaatt caactgatcg
     2881 ccaactttta cttttttatc atcagtccaa agtaatgaaa catgcatgtg aggcggctca
     2941 gcaaaaggca aattttttcc atttaattta aatggtgagc gaactttacc tattgcctgg
     3001 gcaatacctt ctgctaccgc aacacttttg ccgcgtaggg ttgaacgatt agttggcgct
     3061 gctagcgcaa ctccgtgcgc tgtacctcca cttacgacaa gtagtcgcgc attaccgtgt
     3121 gcatcgactt gacgatagcc aacgtgttcg tggtcaccag ttaattcatg gatttctaaa
     3181 actgtgccag taatttcgag cgcttttaat tcacccaacc acagatgggt accaagtctt
     3241 acttccaacg aaatatttag atttcgttct ttagtaattt cttggatttc tctaatatct
     3301 tttgcactca cgtgcgaaag cgaaatatgc atcggccagt tcattttttc tgttaccgaa
     3361 gcaaaactac ttaaccagct ggcgatttct attattcgac tggttgattt tttagaattt
     3421 aatttggcac ttccttcaag tgtgggtaac agcacattta ccggatctga aattggtgaa
     3481 tgcagactta agccaagtat ttcaatgttg tggtgatcac catttgcgat cgaaacaagt
     3541 tctgcggcag atattccaaa acgatgaagt gaagttctac cctcaataaa agccttttta
     3601 attccaactc gtgaggcctg cgcaaaatca ggacccgaaa gagtagcgat gacgcgatga
     3661 gccccaagcg gcatcaaccg ttcccaagcg gcaatagcag gttcgtcatg cggattaaac
     3721 ggttcaagga caactatttc atcgccaaaa tccgtcaagg cttgatctaa ttcgtagata
     3781 gttcctacag caattcgctt taacccaagc acagtcgatt ctttagccag taatcgacga
     3841 cctaaaccgt agccattgcc tttaataacg ggagttacag ttgcaccaga tttttcaaat
     3901 tgctcaagcg ttgaagcaag atgcttattg aatatttttc gatcaatagt taatgtgaaa
     3961 gtcattcaac taccgcctcg acatatacaa attaaatgca gtggaccaac ttttagaaat
     4021 aacgtaatcc cactcaccta catattcttg cgcatatccg ctggtaccaa gtttgaattg
     4081 aattaaccca aacagatggt tattttgatc aagcgtgtca gcaattccac gcatgtcgta
     4141 aatttcactt ccagaatgca aactatcttt catcatttcc cattgaatag cattactggg
     4201 tcgcaagtct cgtgcggacg tagttgaagc tccgtatgaa taccaggaat ggtttccgac
     4261 tcgagtaaga gttgtcgcag ctatcgcacc ttgatgatct ttatgttctg ctataaaaag
     4321 ttttattcgg cctggatctt cggaactaag tgcttgccac atagttcgaa agtaacttag
     4381 aggtcgaggg gtaaaacgat cccgttttgc tgtttctata taacaagtat gaaatagctc
     4441 caaatccgtt tcattgccta agcgaaccgt gactccatca ttttgcgcct ttttaatatt
     4501 gcgccgccac tgttgattaa atgaatttag taagtcctct tcagttttat tttttaagct
     4561 cacctggaat acgaacctag gttgatagtc gccaaaactt gcttgatctg cctttggttg
     4621 cttccagcca aggtgcttaa gttgggttac taaattaatt cctacatgat ttgtttcatc
     4681 aggatttaat tcacttattc gttttgcaga ctcatttgcg attgcatctt ttaaagtttg
     4741 tgcggaccat ctacggaccc aaacatgtgg acccattttt atttgaaaga ctccaaaagg
     4801 ttttagatat tcaatcaacg catttaatgc accagataaa tcttgctgag cccaatctaa
     4861 atctggccct tcaggtaagt aagctagata cttatttagt ttgggaacct tgcgaagtaa
     4921 aactaaacca gcaccaatta gttggccatc ttcaaaccaa cctaatgact tacgttgcca
     4981 accaactttc gtttcacccc aggccggcgt ttgcaaaaaa gaaactgacg gtctggagtt
     5041 t
//
LOCUS       DBNHVN010000079         8664 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_14525, whole genome shotgun sequence.
ACCESSION   DBNHVN010000079 DBNHVN010000000
VERSION     DBNHVN010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 8664)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8664)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8664
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_14525"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /gene="rplL"
                     /locus_tag="AC16Y1_01415"
     CDS             complement(<1..377)
                     /gene="rplL"
                     /locus_tag="AC16Y1_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010550346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR5831156.1"
                     /translation="MAKLSTDQLIDAFKELTLIELSEFVSQFEEVFNVTAAAPVAVAA
                     AAPAAGGGDAGGGAAQDTFDVILEDGGAQKIAVIKEVRALTNLGLKEAKDLVEGAPKP
                     VLEGAKKDAAEAAKAQLEGAGAKV"
     gene            complement(451..1128)
                     /gene="rplJ"
                     /locus_tag="AC16Y1_01420"
     CDS             complement(451..1128)
                     /gene="rplJ"
                     /locus_tag="AC16Y1_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR5831157.1"
                     /translation="MARADKAAAVAEITEEFRASNAAVLTEYRGLTVGQLKELRRSLG
                     PDTTYAVVKNTLSKIAAKDAGVEGFDSLLQGPTAIAFIKGDPVTAAKGLDTFAKDNPL
                     LVIKGGIMDGNILTADDLKKLAKLESREVLLSKVAGAANAALAKAAALFQAPLSKTAR
                     TIAALEAKGGAAGETAAAAPVVEAQAPAAVEEEVSVAAEEAPAVAEDTPAETASAESA
                     EEETTEG"
     gene            complement(1356..2069)
                     /gene="rplA"
                     /locus_tag="AC16Y1_01425"
     CDS             complement(1356..2069)
                     /gene="rplA"
                     /locus_tag="AC16Y1_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR5831158.1"
                     /translation="MKRSKAYRNTAAKINKDEQYSPLTAVRIAKQGATSTKFDQTVEV
                     SMLLGIDPRKADQMVRSTVNLPHGTGKTARVLVFAGGQKADEARAAGADYVGVDELID
                     KVAGGWTDFDAVVATPDLMGKVGKLGKVLGPRNLMPNPKTGTVTLDVAKAVEDIKGGK
                     IEFRVDKHSNLHFIIGKASFSEKQLVENYAAALDEVLRVKPSSSKGRYIKKAVLSTTM
                     GPGLQIDTNKTSNLLAEDE"
     gene            complement(2154..2585)
                     /gene="rplK"
                     /locus_tag="AC16Y1_01430"
     CDS             complement(2154..2585)
                     /gene="rplK"
                     /locus_tag="AC16Y1_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR5831159.1"
                     /translation="MAPTKKVVGLIKLQIKAGEANPAPPVGPALGQHGVNIMDFCKAY
                     NAETEGQRGQVIPVEISVYEDRSFTFVTKTPPAAKLILKAAGIEKGSGVPHTNKVANI
                     TKDQVRDIAQTKLVDLNANDIDAAMKIIEGTARSMGITVSN"
     gene            complement(2614..3558)
                     /gene="nusG"
                     /locus_tag="AC16Y1_01435"
     CDS             complement(2614..3558)
                     /gene="nusG"
                     /locus_tag="AC16Y1_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014144281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR5831160.1"
                     /translation="MTDPQFMEAGSVKEAAALDEAETFTESVETPIDSDAAIETVTND
                     WNGQVDVDAEGEITSDLTGDLASDNSFDISQIVEQELSSDAAVVSADAAEEELAQSSA
                     NEEEDPIVAFRARLESQFGDWFVVHSYAGFENRVKQNLETRTVSMNMEDYIYEIAVPQ
                     EEVTEIKNGVRKLVKRNKFPGYVLVRMDLTDESWGVVRHTPGVTGFVGQGHNPAPLSF
                     EEAFEMLRPSKEKPVATVASAAASKAKAKSAPGAPAPKIDIDLSIGDSVTVVDGPFAT
                     LHATISEINLDAQKVVGLVEIFGRETPVELGFNQIHKN"
     gene            complement(3575..3790)
                     /gene="secE"
                     /locus_tag="AC16Y1_01440"
     CDS             complement(3575..3790)
                     /gene="secE"
                     /locus_tag="AC16Y1_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIR5831161.1"
                     /translation="MSSATDEKTGFFRRIPLFLRQVNAELRKVVWPTKPQLVTYTTVV
                     IVFVVLVAILVSILDLAFARLMLFVFG"
     gene            complement(3839..3911)
                     /locus_tag="AC16Y1_01445"
     tRNA            complement(3839..3911)
                     /locus_tag="AC16Y1_01445"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3876..3878),aa:Trp,seq:cca)
     gene            4045..5241
                     /locus_tag="AC16Y1_01450"
     CDS             4045..5241
                     /locus_tag="AC16Y1_01450"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020132678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR5831162.1"
                     /translation="MTKPRISARISAIAESATLAVDAKAKALKAAGRPVIGFGAGEPD
                     FPTPDYIVEAAVAACRDPRWHRYTPAGGLPELKSAIAVKTLRDSNYKIDASQVLITNG
                     GKQALYNAFATLLDPGDEVLLPAPYWTTYPEAISLAGGKPVEIMTDESTGYRTSVMEL
                     DKALTPKTKALVFVSPSNPTGAVYSPAEVEAIGQWAVEKGIWVITDEIYEHLVYGDAE
                     FTSMPVLVPELADNCIVVNGVAKTYAMTGWRVGWMIGPNDVVKAATNLQSHATSNVAN
                     ISQIAALAAVSGDLKAVDEMKVAFDRRRKTITKMLNEIEGVNCPEPEGAFYVYPSVKN
                     VLGKTFRGKTPTTSAELATLILDEVEVAVVPGEAFGTPGYIRLSYALGDDDLIEGVSR
                     IQDFLK"
     gene            complement(5242..6474)
                     /locus_tag="AC16Y1_01455"
     CDS             complement(5242..6474)
                     /locus_tag="AC16Y1_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family ATP-binding protein"
                     /protein_id="HIR5831163.1"
                     /translation="MINVIVAVGQTEWEAQFVSGLTHPMTGIQVQRRCVDAVDVLAVI
                     KVLSCDAVIISDHTLRLDAEFVAEIQSQNIRVIALTNKANYFEQLGVRETVLLDLSNP
                     LAAIPIISAMVRVNKNNLEPETAPTGELIFVGGFGGGTGKTRLAAELSYQLASQNNHT
                     LLIDGDTYGPVMFQLFGLAPSGVGLLEICRKLERNTAGEKFLNSTATNLLPNLELVGG
                     ISKSSRWIDLRPAIVNDFWQLCSHEYQTVVIDGGPVIEIEPLLAIETGIPKRNLVANS
                     ALLASQKVILTCRAQSLSVTKLIKGLAENNGLFANKDLTIALLSGPTKKHTKDSIYAI
                     ASHTDCENLLVVEHDEALIEKALGQNTLVSDLSPKSQLAHSYADLAKAIFKANKISGT
                     SGRFDRMFAKRLALNGAN"
     gene            complement(6471..7094)
                     /locus_tag="AC16Y1_01460"
     CDS             complement(6471..7094)
                     /locus_tag="AC16Y1_01460"
                     /inference="COORDINATES: protein motif:HMM:NF020250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HIR5831164.1"
                     /translation="MKRAKTGKPKRSVRDFRLWLGLSFILISIGLTHNVLSSATARSQ
                     AVVVTNEIPAGSVISQSDLEIVEVVLPTQVKAVTDYAQAVGSTATRDLFIGDVLTNSS
                     ISSKTNSNVRLVSVPIRAGHLPKLDSGQLVDIWMTPSTDGMALPGPAQLLISQASISE
                     IPDGIDPTLDTAVTLLIPSTQVQVLVQAMRDGVIDIVALPENRRPTK"
     gene            complement(7081..7647)
                     /locus_tag="AC16Y1_01465"
     CDS             complement(7081..7647)
                     /locus_tag="AC16Y1_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831165.1"
                     /translation="MDQQNLVATEHGVLVQANEILNQAAQDITIETRIEKLIEVNQIV
                     REEQETVNLIDRIRNTAGEIKLHFVQKELPNLAGEIQFTSEDFLVLKQAKTQYLIALK
                     QVELISGVDDKAIFCNLAQELNTPLLWLKNLVDAQNSVTVQLLSGRTLSGKIGRLSCD
                     HLDLSSSAQIILVPLNSIIFLRCDYEAR"
     gene            7792..8310
                     /locus_tag="AC16Y1_01470"
     CDS             7792..8310
                     /locus_tag="AC16Y1_01470"
                     /inference="COORDINATES: protein motif:HMM:NF039460.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3235 family protein"
                     /protein_id="HIR5831166.1"
                     /translation="MNAEIYALPVPFANYQATANVIALPKRTKYGLQETLPFEWPTKQ
                     PVNYLSPRVTELKPNDFTERPTSRTELPSATQWSMRLVQVLIEILNGNRSASQLIRWV
                     TPEVMTGVKLAIKRKRFSKLIVRSIHVHETDDGVAEVSAVFGTANRAFALALRLEGLD
                     GKWRATSIVWGF"
     gene            complement(8307..>8664)
                     /locus_tag="AC16Y1_01475"
     CDS             complement(8307..>8664)
                     /locus_tag="AC16Y1_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HIR5831167.1"
                     /translation="KDPLVEYQREGYDLFMAMMDAIKEESVGFLFNVEVKVSTQKDFD
                     GDGKVDEVQVEAKGLDVNQAPTQLSYSAPSETGEVKASVSRTTSSEFENVGRNDECPC
                     GSGKKFKKCHGDPANR"
BASE COUNT         2232 a         1992 c         1866 g         2574 t
ORIGIN      
        1 acttttgcgc cagcgccttc tagttgtgcc ttagcagctt ctgctgcatc tttcttggca
       61 ccttcaagaa ctggttttgg agcaccttca actaggtcct tagcttcctt tagacctagg
      121 ttggttagtg cgcgtacttc cttgataacg gcaatcttct gtgcgccgcc atcttcaaga
      181 attacatcga aagtatcttg tgctgcgccg ccacctgcgt cgccgccgcc agctgctgga
      241 gctgctgctg caactgcaac aggagctgct gcggttacat tgaatacttc ttcgaactgt
      301 gaaacgaatt ctgaaagttc aatcagagtt agttccttga atgcatcgat caactgatct
      361 gtgcttagct ttgccatttc ggcgtccttc cggtgtgggg ctttaaggcc cggtttaatc
      421 actcacactt tgcaagtgac tttgaggggc ctagccctcg gttgtttctt cttctgctga
      481 ttcagcggac gccgtttcag ctggagtatc ttctgcaact gctggtgctt cttcagcggc
      541 cacagaaact tcttcttcta ctgcagcagg tgcttgggct tcaactactg gagcagcagc
      601 cgcggtttca ccagcagcgc cccccttagc ttcaagcgca gcgatagtac gagcagtctt
      661 tgatagtggt gcttggaaaa gtgcagcagc tttggcaagt gcagcgtttg cagcgccagc
      721 aactttggaa agcaatactt cgcgtgactc aagcttggct agcttcttaa gatcatcagc
      781 ggtcaagatg ttgccatcca tgattccgcc cttgattaca agaagtggat tgtctttagc
      841 aaaagtatct agaccctttg ctgcagttac tggatctccc ttaatgaatg caatcgctgt
      901 tggaccttga agtagtgaat caaagccttc aacacctgca tctttagcgg cgatctttga
      961 aagtgtgttt ttaacgacgg cgtaagtggt gtcaggtcca agtgaacgac gtagttcctt
     1021 taactgaccc acggtcaaac cgcggtactc ggtcaaaact gcagcgtttg atgcacggaa
     1081 ttcttccgtg atttctgcaa ctgcagctgc cttgtcagcg cgtgccatgg tgctcctttt
     1141 gtaggttgct ccataaaaaa agccccgcgc aagcgaggca taaataaagt gtctaaacac
     1201 ttacttctat actcacctgc gctggcgctc cggttttgga gccttagcct caccttgtta
     1261 ggtgatgacc ggcggtcttc ggtttgttct tgactaactt taggctttct gggctctgcg
     1321 gtcaaaattg atgcagagcc cagaaaccct tcaaattatt cgtcttcagc taataggttg
     1381 ctggttttgt tggtatctat ctgtaggcca ggtcccatag ttgtcgaaag aactgctttt
     1441 ttaatgtagc ggccctttga cgatgatggc ttcacacgta gtacttcatc aagagctgca
     1501 gcgtagtttt caactaactg tttttctgag aaggatgctt tgccaatgat gaagtgcaag
     1561 tttgagtgct tatcaacacg gaattcaatc tttccgccct tgatatcctc aactgccttc
     1621 gccacgtcta gcgttacagt tccggtcttt gggtttggca ttaggttacg tggaccaagc
     1681 accttaccta gcttgccgac tttacccatt agatctggag ttgctaccac tgcatcgaag
     1741 tcagtccagc caccagcaac tttgtcgatt aattcatcga cgccgacata gtcagcacct
     1801 gctgcgcggg cttcatctgc cttttgtcca cctgcaaaga ccaggacgcg tgcggtcttt
     1861 ccggtgccat gtggcaagtt aactgtgctt cgaaccattt ggtctgcttt acgtggatcg
     1921 atacctaaaa gcatcgaaac ttcaacagtc tggtcgaact tggtgctagt tgcaccttgc
     1981 ttggcaatac gcaccgcagt tagcggactg tattgctcat ctttattgat tttggctgcg
     2041 gtattccggt aagccttgct tcgcttcatt tctttctcct cttagttatg gagttcgtgg
     2101 tgcgaacctg aagcaggctc tcccactgtt tattggatat ttagttcttc tgattaatta
     2161 cttacggtta tgcccattga acgagcagta ccttcaataa ttttcattgc ggcatcgatg
     2221 tcatttgcat tcaagtcaac caacttagtc tgagcgatat cgcgcacctg atctttggtg
     2281 atgtttgcaa ctttgttcgt gtgtggaaca cctgaacctt tttcgattcc ggctgcttta
     2341 agaattaact ttgcagcagg tggagtcttg gtaacgaatg tgaaagagcg gtcttcataa
     2401 accgaaattt caactgggat tacctgacca cgttgacctt ctgtttcagc gttgtacgcc
     2461 ttgcagaagt ccatgatgtt gacgccgtgc tgacctagcg caggaccaac tggtggagca
     2521 ggatttgctt caccagcctt gatctgtagc ttgatcagac cgacgacctt ttttgttggt
     2581 gccatgtctt atgcgtcctt tttcttttgt ttcttagttc ttgtggattt ggttgaagcc
     2641 aagttccaca ggagtttcgc gaccgaagat ctctacgaga ccaacgacct tttgtgcatc
     2701 caagttgatc tcggaaatcg ttgcgtgcaa tgttgcgaat ggtccatcta caacagttac
     2761 agaatcgcca atgcttagat cgatatcgat ctttggtgca ggtgcacctg gagcagattt
     2821 ggcttttgcc tttgatgctg ctgccgaagc aacagttgcc actggctttt cttttgaagg
     2881 acgtagcatt tcgaatgctt cttcaaatga aagtggagct ggattatgac cctgccctac
     2941 gaaaccagtt acgcctgggg tgtggcgaac aacgccccat gactcgtcag ttaagtccat
     3001 gcgcacaagc acgtaacctg ggaatttgtt gcgcttaaca agctttcgca caccattctt
     3061 aatttcagtt acttcttcct gtggcacagc aatttcgtaa atgtaatctt ccatgttcat
     3121 ggaaaccgtg cgggtttcaa gattttgctt tactcggttt tcaaatcctg catatgagtg
     3181 aacaacaaac cagtcaccaa actgagactc taagcgcgca cggaaagcaa caatcggatc
     3241 ttcctcttcg tttgccgaag actgcgcaag ttcttcttcg gcggcatcgg ctgaaacaac
     3301 agcggcatcg gatgaaagtt cttgttcaac tatttgcgaa atatcaaatg agttgtcgct
     3361 agctaagtcg ccagttaaat cacttgtgat ttcgccttca gcatctacgt ctacttggcc
     3421 attccagtca ttagtaacag tctcaatcgc agcatcgctg tcgatcggtg tttctactga
     3481 ttctgtaaaa gtttctgctt catctagcgc agcagcttct tttacggaac cggcttccat
     3541 aaattgcgga tcagtcacat taattcctta ctacttatcc gaaaacgaaa agcattagtc
     3601 gagcaaatgc taaatctaaa attgaaacca aaattgcaac taaaacaaca aagacaataa
     3661 ccacagttgt gtacgtaact agttgtggct ttgttggcca aacaactttg cgtagttccg
     3721 catttacctg gcgaagaaac agcggaatgc gacggaagaa accagtcttc tcgtcagttg
     3781 ctgaactcat gtcttgcctt tcttttttta ctggcgcggt taggcgccgt tataaaagca
     3841 gggccggagg gacttgaacc cccaaccgcc ggttttggag accggtgctc taccaattga
     3901 gccacgaccc ttggtcctgc tttagatcgt taggtcattt tgggcatgcc gaaataagcc
     3961 taagatttgt gcggtgtgtc aagtgtaagg gatatttctc atcccaaata ccacgcgatt
     4021 ttgccgatag acaatactta acttgtgact aaaccccgca tttccgccag aatttccgcg
     4081 attgcagagt ctgcaactct cgctgtcgat gctaaagcca aggcgctaaa ggctgccggt
     4141 cgacctgtaa ttggttttgg tgcgggcgaa cctgattttc caactcccga ttacattgtc
     4201 gaagctgcag ttgctgcttg ccgcgatcca cgttggcatc gctacacccc agctggtggt
     4261 ttacctgaac taaaaagtgc cattgcggta aaaactttgc gcgattcaaa ttacaaaatt
     4321 gatgccagcc aagttctaat tacaaatggt ggcaagcaag cgctttacaa cgcatttgca
     4381 actctgctag atcctggtga tgaagtttta ttgccggcgc cttattggac aacttatcca
     4441 gaagcaatct cgcttgccgg tggcaagcca gttgaaatta tgactgatga atcaactgga
     4501 taccgcactt cagttatgga attagataaa gcgttaactc caaaaactaa agcacttgtt
     4561 tttgtttcac caagtaatcc aaccggcgct gtttattccc ctgccgaagt tgaagctatt
     4621 ggtcaatggg ccgttgaaaa aggtatttgg gttattacgg acgagattta tgagcacctg
     4681 gtttacggtg atgctgaatt tacttcgatg ccagttttag ttccagaact tgctgacaac
     4741 tgcattgtcg ttaatggtgt ggcgaagact tacgccatga ccggttggcg tgtgggctgg
     4801 atgattggtc caaacgatgt agttaaagct gcaacgaatt tacaatcaca cgcaacttca
     4861 aacgtggcta acatttcgca gattgcagca cttgccgcag tgtctggtga cttaaaagca
     4921 gttgacgaaa tgaaagtggc ttttgatcgt cgacgcaaaa caattaccaa aatgcttaat
     4981 gaaatcgaag gcgtaaattg cccagaacct gagggtgctt tctacgttta cccgtcagtt
     5041 aagaatgttc taggtaaaac tttccgcggc aagactccta caacttcggc cgaacttgcc
     5101 actttgattt tggatgaagt tgaagttgcg gtggtaccag gtgaagcttt tggcactcct
     5161 ggttacattc gactttccta tgcacttggt gatgatgacc taatcgaagg cgtatctcga
     5221 attcaggact ttcttaaata attagttggc tccatttaat gcgagtcgct tagcaaacat
     5281 ccgatcgaat cttccagaag tgccactgat tttattggcc ttaaatattg cctttgctaa
     5341 atctgcatac gagtgggcta gctgcgattt aggtgataaa tcacttacca gagtgttttg
     5401 acctagcgct ttttcaatta atgcttcgtc atgttcaacg actaacaaat tttcacaatc
     5461 ggtatggctt gcaattgcat atattgaatc tttcgtatgt tttttagtag gaccactaag
     5521 taacgcaata gttaaatctt tatttgcaaa tagtccgttg ttttcagcta atcctttaat
     5581 taacttagtc acgctcagac tttgagcgcg acaagtcaga ataacttttt gactagctag
     5641 taatgcagaa tttgccacca aattacgttt cggaattccc gtttcaattg caagtagcgg
     5701 ttcaatttca attaccggac caccatcaat aactactgtt tggtattcat gcgaacacag
     5761 ctgccaaaaa tcatttacaa tcgcaggtcg caaatcaatc catctagaac ttttacttat
     5821 tccgccaact aattccaaat taggcaacaa attagttgcc gttgaattta gaaatttttc
     5881 accagcagtg tttcgctcaa gtttccgaca aatctctagt aacccaacgc cagaaggtgc
     5941 aagaccaaat agttgaaaca tgaccgggcc gtaagtatca ccatcaatta acaaagtgtg
     6001 attgttttgg ctggctagtt gataactaag ttcagcagct aaccgagttt taccagtacc
     6061 accaccaaat ccaccaacaa aaattaattc acctgtaggt gccgtctctg gttctagatt
     6121 atttttgtta actcttacca tcgctgaaat tatcggaatt gcagccaaag gattacttag
     6181 atcgagcaga actgtttcac gaacacctag ttgttcaaag taatttgcct tattagtcag
     6241 tgcgataact cgaatatttt gactttgtat ttcagccaca aattctgcat ctaaacgcaa
     6301 agtgtgatcg gagattatca ctgcatcaca cgacagaacc ttgattacgg caagcacatc
     6361 gactgcatcg acacatcggc gttggacttg aatcccagtc atcgggtgag ttaagccact
     6421 tacaaattgt gcttcccact cggtttggcc aactgccaca atcacattga tcattttgtt
     6481 ggccggcgat tttctggaag cgccacaatg tctattacgc catctcgcat cgcttgaact
     6541 aatacctgaa cttgggtact tggaataagc aatgtgactg cggtatccaa agttggatca
     6601 attccatctg gaatttcgct aattgatgct tgtgagatta ataattgcgc cggtcccgga
     6661 agtgccattc cgtctgttga cggggtcatc caaatatcaa ctaactgacc agaatcgagt
     6721 ttaggtaggt gtccagcgcg gattggcaca ctaaccaagc gaacattcga attagttttt
     6781 gaacttatcg aactattagt taatacatcg ccgataaata aatctcgggt cgcagttgag
     6841 cccactgcct gcgcataatc agtaactgct ttaacttgag tcggcaagac tacttctaca
     6901 atttctaaat ctgattgtga aatcacactg ccagccggaa tttcgttagt taccacaaca
     6961 gcctgtgacc tggcggtagc gctactcaaa acattgtggg ttaatccaat tgaaatcaaa
     7021 atgaatgaaa ggccaagcca caatcgaaag tctctaactg aacgcttcgg cttaccggtt
     7081 ttagcgcgct tcataatcac accgcaagaa aataattgaa tttagcggaa ccaaaatgat
     7141 ttgtgcagag ctcgataaat ctagatgatc acaactaagt cggccaattt ttccggaaag
     7201 agttctgcca ctaagtaatt gaactgttac tgaattttgt gcatcaacta aatttttcag
     7261 ccagagtagc ggtgtattta attcttgagc caaattgcaa aaaatggctt tatcatcaac
     7321 accagaaatc aattccacct gctttagggc aattaagtac tgagtttttg cttgcttaag
     7381 tacgagaaaa tcttcggaag taaattgaat ttcaccggct aagttcggca actctttttg
     7441 aacaaaatga agtttgattt caccagctgt atttcgaatt cgatcaatta aattaacagt
     7501 ttcttgttct tctctgacta tttgattaac ttcaattagt ttttcaatcc gagtttcgat
     7561 cgtaatgtct tgagctgctt gatttaagat ttcgtttgct tgcactaaaa caccatgttc
     7621 agtggcaact aggttctgct gatccatttg aaaattttgc ccaaatggtt attcaatctg
     7681 cattagttat ccacagaaag gtaaaaaata actattccct cttgacccaa atttttggca
     7741 gacatagaac tgccctacag acctagtggt ggcctggggt tcggaggaat tatgaacgca
     7801 gaaatttatg cactgccggt gccatttgca aattatcaag caaccgcaaa tgttattgcg
     7861 ctaccaaagc gcactaaata tggacttcaa gaaacgttgc catttgaatg gccgactaaa
     7921 caaccagtta actatctgtc accccgagta acagagttaa agcccaacga ctttactgaa
     7981 cggccaacta gcagaactga attaccttca gccacgcaat ggtcaatgcg cttagttcaa
     8041 gttttaatcg agatactcaa tggcaatcgc tcagctagtc aattaattag gtgggtaaca
     8101 cccgaggtga tgaccggtgt aaaactggcg ataaagcgaa aaaggttttc taagttgatt
     8161 gtgcgcagta tccatgtgca tgaaacagat gatggggtcg ctgaagttag tgcagtattc
     8221 gggactgcaa acagagcttt tgcgttagca cttcgcctag aagggttaga tggaaaatgg
     8281 cgagccactt ctatcgtttg gggtttctag cggttggctg gatctccgtg gcactttttg
     8341 aatttcttgc cagatccaca tggacattca tcgttgcgtc caacattttc aaattctgag
     8401 gaagtagtcc gggaaactga agctttaact tctcctgttt cagatggcgc tgaataggaa
     8461 agttgagttg gagcctggtt tacatctaac cctttagcct caacttgaac ttcatctact
     8521 ttgccgtcac catcaaaatc tttttgtgtt gaaactttta cttcaacatt aaataagaat
     8581 ccaaccgact cttctttgat ggcatccatc attgccataa ataagtcgta accttcgcgt
     8641 tggtattcaa ctaatggatc tttc
//
LOCUS       DBNHVN010000080         1674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_88897, whole genome shotgun sequence.
ACCESSION   DBNHVN010000080 DBNHVN010000000
VERSION     DBNHVN010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_88897"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..178
                     /locus_tag="AC16Y1_01480"
     CDS             <1..178
                     /locus_tag="AC16Y1_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020216434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR5831168.1"
                     /translation="GLNQGEVAGAGVAGHIHQHVVPRWANDSNFMPVIGQTKVMSELI
                     EDTRIRISNAWTSN"
     gene            complement(155..1114)
                     /locus_tag="AC16Y1_01485"
     CDS             complement(155..1114)
                     /locus_tag="AC16Y1_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831169.1"
                     /translation="MMLPTSITSSVDQSKLPIVVGVLGASSEILTKLNGLLPANCAVL
                     SLTETLVTESVVSSCDAIIGILDGDLGLSADLISSWNLAKDHDLPRLILATNTVEGRA
                     DFDEALALAELVLNEDIAIRYFPIVADDQAKYIGLLDVLSQEIVLPDGTTQPADPEHI
                     ELTTDDHNELIELLLHSDLDNDLIIKHEDGSPISFPRLRELWQQSHLVTVLPIDQWVS
                     ALQITLWFANLNPLWIPDVFEDGQGKDLTDCHDTLGIGIALGIARMWPSANDEALEII
                     TKDNEHIGIEVNSKFPIFFDSRIRPDDVIRPKHSNYLVTRPRI"
     gene            1151..>1674
                     /locus_tag="AC16Y1_01490"
     CDS             1151..>1674
                     /locus_tag="AC16Y1_01490"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR5831170.1"
                     /translation="MLDNGKMRKISSKTIDPIARFLLNKGISADFITVIGALLAALIC
                     IFYLSQGEFFIGGVLLSIVGISDLLDGTMARLSNTAGPWGAFLDSTLDRVVDAFIYGA
                     ILYWVATQPTFNALLFSAAVIGAFMAQVTSYTRARWESVGVIGKVGLVERSERMIALC
                     TAFILTGFGIDIIA"
BASE COUNT          462 a          335 c          371 g          506 t
ORIGIN      
        1 tggtttgaat caaggtgaag tggcaggtgc cggagtcgct ggtcacattc atcagcacgt
       61 tgttccacgc tgggcaaatg atagtaattt catgccagtg attggtcaga caaaagttat
      121 gtctgaattg attgaagata caagaattag aatctcaaat gcgtggacga gtaactaaat
      181 aatttgaatg ttttggacga attacgtcat caggccttat tcgactatca aaaaaaatcg
      241 gaaactttga atttacttct attccaatgt gttcattgtc tttagtaatg atttcaagtg
      301 cttcatcgtt tgcacttggc cacattcgag caattccaag agcgattcct attcctagag
      361 tgtcatggca atcagtcaaa tctttacctt gaccgtcttc aaaaacatcg gggatccaga
      421 gtggatttag attggcaaac cacaaagtga tttgcaacgc actgacccat tgatctattg
      481 gcaaaaccgt taccaagtga ctttgttgcc agagttcacg cagtcttggg aagctgattg
      541 gacttccatc ttcatgttta ataatcaagt cattgtctaa atcgctatgt agcaatagct
      601 ctattagttc gttgtgatca tctgtagtta actcgatatg ttcagggtca gcaggctgag
      661 tagttccatc aggaagaacg atctcttgac tcagcacatc tagcaagccg atgtactttg
      721 cttgatcatc agcaactatt ggaaaatacc taatggcgat gtcttcattc agaacaagtt
      781 cagctaacgc cagggcttca tcaaaatcgg ctcgaccttc gaccgtatta gtcgccaaga
      841 ttaatcttgg taaatcatgg tcttttgcta aattccagct tgaaattaga tctgcgctta
      901 aacctaaatc gccatccaag atgccaataa ttgcatcaca actagaaaca acagattccg
      961 taaccaaagt ctcagttagg gacaagaccg cacagtttgc gggtaaaagc ccattgagtt
     1021 tggtaaggat ttcactagat gcgccgagaa ctcccaccac gattggcaat tttgattggt
     1081 ccactgagga agtgattgaa gtcggtaaca tcatgtgtag ttaatgctag ttgagtttta
     1141 ggggatcgcg gtgctggata acggcaagat gcgaaagatc agttcaaaaa ccattgatcc
     1201 gattgcccga tttttactca acaaaggtat ctctgcagat ttcataacgg taattggagc
     1261 tttacttgca gctctgattt gtatttttta tttaagtcaa ggtgaattct tcattggggg
     1321 agttctatta agcattgtcg gaattagcga tttacttgat gggaccatgg ctcgactgag
     1381 taatactgcc gggccttggg gtgcattcct ggattcaaca ctagatcggg tagtcgatgc
     1441 atttatctac ggagcaatcc tttattgggt cgctacccaa cccacgttca atgcgttact
     1501 tttcagtgca gcagtgatcg gtgcatttat ggctcaagtt acttcttaca ctcgagccag
     1561 atgggaatcg gtgggagtta tcggaaaagt tggtttggtt gaacgttcag agcgcatgat
     1621 tgcactttgc acagcattta tccttactgg atttggaatt gacattattg cctt
//
LOCUS       DBNHVN010000081         3289 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_460090, whole genome shotgun sequence.
ACCESSION   DBNHVN010000081 DBNHVN010000000
VERSION     DBNHVN010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_460090"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="AC16Y1_01495"
     CDS             complement(<1..790)
                     /locus_tag="AC16Y1_01495"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009326720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5831171.1"
                     /translation="MSDVLSATGDVKLKGLRKVYGDFVAVHNLDLVIPQGSFFALLGP
                     SGCGKTTTLRMVAGLDEPTSGQVFIGDTDITHTKPYQRPVNTVFQNYALFPHMDIFEN
                     VAFGLRRRGEKNVDQAVSDALELVQLSHLAKRKPGQMSGGQQQRIALARAIVNRPQVL
                     LLDEPLGALDLKLRRQMQLELKRIQLEVGLTFVHVTHDQEEAMTMADTIAVMNNGEIE
                     QLGSPTELYENPQTAFVANFLGQSNLFRATIESIDGENVVASDKD"
     gene            complement(800..2020)
                     /locus_tag="AC16Y1_01500"
     CDS             complement(800..2020)
                     /locus_tag="AC16Y1_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013349917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5831172.1"
                     /translation="MENLNQEQATNFLKNAMSRRGFLGAAGAVAATGALAACGTGGTA
                     TPAATGSGTATTAFPTDVSDTEKIVRWANWPLYLDYDEAADTYPTLDAFSAASGIKAT
                     YKEEIDDNNSFWGKVNGQLKTGKDIGFDVVTPTDWMASRFIQYGYAQPLDAAKIPNKS
                     NILDGLANVTFDKGRNYSLTWQSGFAGLVWNKEKLPNGLQSVEQLWSDELKGKVTVLS
                     EMRDTMGILLMSLGIDIAAGISEAQFQQGLDLLSEKLGDGSIYKVQGNEYAKDLENKQ
                     AWAGIVWSGDIFVLNAEEGDQWGFALPDSGGTLWSDNMLIPSTSSHQSNAMALMNHYY
                     DPAVAAQVAAYVNYICPVKGAQAEMEKIDPALAASEFIFPTDETLAKASVFPALDGAT
                     EQLFSDLWATTTGV"
     gene            complement(2134..>3289)
                     /locus_tag="AC16Y1_01505"
     CDS             complement(2134..>3289)
                     /locus_tag="AC16Y1_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018102358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5831173.1"
                     /translation="FAVTAAEEIPPMIDQIRFFAGAARVLEGRSAGEYMKGFTSIIRR
                     EPIGVIGQVTPWNYPMMMAVWKFAPAVAAGNCVVLKPSDTTPASTVRMAEIIGDILPP
                     GVLNVVTGDRDTGRALVEHDIPQMVSITGSVRAGMEVAGSAARDVKRVHLELGGKAPV
                     VVFEDADIEAAAEWLAVAGYFNAGQDCTAATRVLVSEKIYGDFVEALAGQAKGLTTTY
                     DNGPTDGDALVPPVNNVNQMEKVLGFLDRVPKHARVAAGGSRIGDRGFYVEPTVVADL
                     KQDDEMIQNEIFGPVMTVQKFSDETEALKWANGVKYGLASSVWTKDLGRGLRMAKYLD
                     FGAVWINTHIPLVAEMPHGGFKHSGYGKDLSMYGFEDYTRIKHVMASLEG"
BASE COUNT          870 a          812 c          697 g          910 t
ORIGIN      
        1 catctttatc cgatgcaact acattttcac catcaatcga ttcaatggtt gcgcggaata
       61 gatttgattg acctagaaag ttagccacaa atgcagtttg tggattttca tatagttctg
      121 ttggtgaacc aagttgttca atttcaccat tattcattac cgcgatggtg tcggccatag
      181 tcatagcttc ttcctggtcg tgcgtaacat gcacgaaagt cagaccaact tctaattgaa
      241 ttcgcttcaa ttcaagttgc atttgacgac gcaactttag atctaatgcg cctagcggtt
      301 cgtctagcaa aagaacttgc ggacggttta cgattgcgcg agccaaagca atacgttgct
      361 gttgaccgcc agacatttgc ccaggtttac gttttgctaa atggctaagt tgcaccagtt
      421 caagtgcatc gctaactgct tgatccacat ttttttcacc acgtcgtcgc aaaccaaagg
      481 ctacgttttc aaaaatatcc atgtgcggga acagagcgta attttgaaac acggtattaa
      541 ctggacgttg gtatggcttt gtatgtgtga tatctgtatc gccgataaac acttggcctg
      601 aagttggttc gtctaaacca gcgaccatgc gcaaagtggt ggtcttaccg caaccagatg
      661 gtccaagtag ggcaaagaaa gacccttgcg ggattacaag gtctagattg tgtaccgcaa
      721 caaaatcgcc atacaccttt cgcaaaccct taagttttac atctccagtt gcagataaga
      781 catcactcat acgtattaat tagactcctg tagttgttgc ccataggtca gagaacaact
      841 gttcagttgc accatcaaga gctgggaaca cacttgcttt cgccaaagtt tcgtcagttg
      901 ggaaaatgaa ttcactggca gctagagctg gatcaatttt ttccatttca gcttgggcac
      961 ccttaactgg gcaaatgtag ttcacgtatg ccgcaacttg ggctgctact gctgggtcgt
     1021 aataatggtt catcaaggcc atggcgtttg attgatgact cgaagtagac gggatcaaca
     1081 tattgtctga ccacaaggtg ccacctgaat caggtagcgc aaatccccac tggtcgcctt
     1141 cttcggcatt aagtacgaag atgtcgccac tccatacaat tccagcccaa gcctgtttgt
     1201 tttctaagtc tttggcatat tcgttacctt gaactttata gattgaaccg tcgccaagtt
     1261 tttctgacaa gagatctaaa ccttgctgga attgagcctc agaaatacct gccgcgatgt
     1321 cgatacctag tgacattaac aaaatgccca tggtgtcacg catttcagac agcaccgtga
     1381 ctttgccctt taattcatct gaccaaagtt gttcaactga ttgcagacca ttaggtagct
     1441 tttctttatt ccaaaccagg ccagcaaaac cagactgcca agtgagtgag tagttacgac
     1501 ctttgtcgaa ggtgacattt gctaaaccat caagaatgtt tgatttgttc gggatcttag
     1561 ctgcatcaag tggttgggcg taaccgtatt gaataaagcg ggaggccatc cagtcagttg
     1621 gagtaaccac gtcaaagcca atgtctttgc cagttttcaa ttgaccatta actttgcccc
     1681 aaaaagaatt gttatcgtca atttcctctt tgtaagtagc tttgattccg gatgcagccg
     1741 agaatgcatc aagtgttggg tacgtatctg cggcttcatc gtaatccaaa tagagcggcc
     1801 agtttgccca gcgcacaatt ttttcggtat ctgaaacatc tgttgggaat gcagttgttg
     1861 cagttccact gccagtagcg gctggagttg cagtgccacc agttccacac gctgctaacg
     1921 cgccagttgc agcaactgca ccagcggcgc cgaggaaacc acggcgagac atcgcatttt
     1981 ttaggaagtt tgttgcttgt tcttgattta ggttttccat acttactttc cttttctttt
     2041 cctaaacaaa ttttctaact ggatttccca gttaggggtt tcctcgtact gctacttaat
     2101 ctacttaact ggccgacaat atgttcagaa gacttaccct tcaagtgatg ccatcacatg
     2161 cttgattcgg gtgtaatcct cgaaaccata catcgataaa tccttgccgt agccagagtg
     2221 cttgaagccg ccatgaggca tttcagctac caatgggatg tgagtgttaa tccaaacagc
     2281 accaaaatca aggtacttag ccatgcgaag gcctcgacct agatctttcg tccagacact
     2341 tgaagcaagc ccatacttaa caccgttggc ccacttcagc gcttcagttt catcactaaa
     2401 tttttggact gtcataactg gcccaaaaat ttcgttttga atcatttcgt catcttgctt
     2461 taagtcagca actaccgtcg gctcaacata gaagcctcgg tcaccaatac gcgaaccgcc
     2521 ggcggcaacc cgagcatgtt taggaacgcg atctagaaag ccaagcactt tttccatctg
     2581 gttaacatta ttgactggcg gcaccaaagc atcgccatca gttggaccat tgtcataagt
     2641 tgttgtgagc ccttttgctt gtccagcaag cgcttcgaca aagtcgccat aaattttttc
     2701 tgataccaaa acccgagttg cggcagtgca gtcttggcca gcattaaagt aaccagcaac
     2761 tgctaaccac tcggctgcag cctcaatgtc tgcgtcttca aaaacaacca ctggtgcttt
     2821 accaccaagt tcaagatgta ctcgcttaac atcacgcgca gcacttccag caacttccat
     2881 accagcacgc actgaaccag tgatcgaaac catttgtggg atatcgtgtt caactaatgc
     2941 gcgaccagta tcacgatcac cagtcactac atttagtacg ccgggaggca aaatgtcgcc
     3001 aataatttca gccatccgca cagttgaggc aggtgtggta tccgaaggct ttaacaccac
     3061 acagttaccg gcagctactg ccggagcaaa tttccaaact gccatcatca ttggatagtt
     3121 ccacggtgtt acttgtccga ttacgccaat tggttcacgg cgaattatcg atgtgaaacc
     3181 tttcatgtat tcgccagctg aacggccttc aagcacacga gctgcgccag caaaaaatct
     3241 gatctgatcg atcatcggcg gaatttcttc tgcagcagta accgcaaac
//
LOCUS       DBNHVN010000082         6623 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1500430, whole genome shotgun sequence.
ACCESSION   DBNHVN010000082 DBNHVN010000000
VERSION     DBNHVN010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6623)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6623)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6623
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1500430"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..435)
                     /locus_tag="AC16Y1_01510"
     CDS             complement(1..435)
                     /locus_tag="AC16Y1_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HIR5831174.1"
                     /translation="MDSSIPGSNKDSALIDKTVRVLVAKAGLDGHDRGAKVVVRALED
                     AGVEVFYTGLHQTPKQIVEAAIENRVGAIGISVLSGAHETVFPEVISLLKEKQREDIV
                     VFGGGVIPQDDIPALLEVGVKIIFTPGTPLSSLTGWAIDNLR"
     gene            730..1719
                     /locus_tag="AC16Y1_01515"
     CDS             730..1719
                     /locus_tag="AC16Y1_01515"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR5831175.1"
                     /translation="MSDTSRGRHRAGRHSAPKKKAVKPKSVSALLGLAGVSSVVVTGG
                     SSLPTLNTNVVNESSDTTTAIPVVKTTAITNNLINRVQAASRTNERETLADAEASAQA
                     STVADPATDILAVIDTTPTLKSEDIRAIQALAGSTEDLITLIDQVQEDANQQAALQVQ
                     QQEIARAAAAEAEAKVAEAIREAESKKQAAIVQAQRIETIGKVLVPVQDGYQLSARFM
                     QRGRIWSGGVHTGLDFRVPVGTRVYAAASGTIVEAGYEGAYGYRIVVDHGDGYMTSYN
                     HLSTIFETGGFITAGTNIGLSGSTGKTTGPHLHLEVLKDGQFINPSGWLWGTD"
     gene            complement(1729..3966)
                     /gene="pcrA"
                     /locus_tag="AC16Y1_01520"
     CDS             complement(1729..3966)
                     /gene="pcrA"
                     /locus_tag="AC16Y1_01520"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005313293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HIR5831176.1"
                     /translation="MSPRERRIVPVPAHLLDDLNEPQRQAVTHAGSPLLVVAGAGSGK
                     TRVLTRRIAYLLAQRNVHPGEILAITFTNKAANEMKERVAHIVGPQARAMWVSTFHSA
                     CVRILRSEAKRLGLRPTFSIYDTQDSVRLMTLVAKDLGIDPKMFSAKYLLSQVSNLKN
                     ELIDYETAVTKAQTTQEQTVAQVYGDYQRRLMMASAVDFDDLIGHTVALFQAFPEVAA
                     MYHRRFRHVLVDEYQDTNHAQYVLIRELVGNGSDGIEPAELCVVGDSDQSIYAFRGAN
                     IRNIMEFERDYSKSTTVVLEQNYRSTQNILSAANAVIDKNVGRPKKNLWTAQGDGEKI
                     VIYSAADEHHEADFIVRKLRELIDQNGFAYKDVAVFYRTNSQSRSVEEVFIRNGIPYK
                     VVGGTRFYERKEIRDALGYLRSIVNPTDDVSLRRIINTPRRGIGDKAEEAVENFARRE
                     KISFSEAVDRVSEINTLAARSQNALKEFSALLNLLRMMDDSGAGPSEIVHTVLESSGL
                     LKELAQSKDPQDEVRIENLSELENVAREFEEQFQAENEENKKARLSDFLERVSLVADS
                     DEIPKDHEHGGVVTMMTLHTAKGLEFPVVFLTGMEEGIFPHQRSLGSETELQEERRLA
                     YVGMTRAMQRLHLSRAASRSTWGQPTYNPESRFLAELPDELVERQGEEFTPLGLSGDF
                     ERPKKRDEQVMVLIVGDRVLHDKFGMGTVTEVRGENEKAEATIDFKSAGIKRLLLRYA
                     PVEKL"
     gene            3983..4744
                     /locus_tag="AC16Y1_01525"
     CDS             3983..4744
                     /locus_tag="AC16Y1_01525"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HIR5831177.1"
                     /translation="MTLNDQNFEIDERFGIPEGVERVLVVTAHPDDVDFGAGGTIATL
                     TELGIAVSYCICTDGDAGGFDDATDRSQIPSIRRAEQIAAGKVLGVTDVNFLGYKDGY
                     LEPNHDVQKDIVRIMRRVKPQLVITQTPERNWERLPATHPDHMAAGEAATRALYPAVR
                     NPYAYPELRNEEGLEAWTVSWLWLQGDSNPNHWLDVTSKFEQKIKAISAHASQVSHLD
                     DLAGMVREWQLLAASRAGLPEGQLAEGFRQIKLPD"
     gene            complement(4773..5123)
                     /locus_tag="AC16Y1_01530"
     CDS             complement(4773..5123)
                     /locus_tag="AC16Y1_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831178.1"
                     /translation="MEFVYNLLVVLHLVGLSSLLGGFLVQMSSATKGINPAMFHGALT
                     QLVTGLGLAGILSAGLLADETVDHTKIGIKLGVSLAVLVVVLIGRKSTTDAKSMWATV
                     GGLTMLNVIVAVFI"
     gene            complement(5126..6622)
                     /gene="guaA"
                     /locus_tag="AC16Y1_01535"
     CDS             complement(5126..6622)
                     /gene="guaA"
                     /locus_tag="AC16Y1_01535"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018889299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR5831179.1"
                     /translation="MRESNIYSEVVPSTMSVADILLKNPLAIVLSGGPSSVYAAGAPQ
                     VDSKLFETGLPVFGICYGFQAMASALGGQVTKTGLSEFGATELTVTKPGCLFAGLPKS
                     QNVWMSHGDSVQVAPDGFEVTASSAGAPVAAFENLTQKFAGVQFHPEVMHSEYGQKVL
                     EHFLYEIAGIKPDWTPAGIVDEQVARIKKQIGDHQVICGLSGGVDSAVAAALVHKAVG
                     DNLTCVFVDHGLLRLGEREQVERDYVAATGIKLVVVDAAQRFLSELEGVTDPEEKRKI
                     IGRLFIRVFEDVQRDLVTQAGNAGNPIKFLVQGTLYPDVVESGGGTGAATIKSHHNVG
                     GLPDDLDFELCEPLRELFKDEVRAVGRQLGLPEEIVMRHPFPGPGLGIRIVGSVNKER
                     LDILRSADAIVREEVTLAGLNSSIWQFPVVLLADVRSVGVQGDGRTYGHPIVLRPVSS
                     EDAMTADWTRLPNELLAKISTRITNEVREVNRVVLDVTSKPPGTIEWE"
BASE COUNT         1862 a         1552 c         1364 g         1845 t
ORIGIN      
        1 ttaacgcagg ttgtcgattg cccaaccagt aagagaagaa agcggtgtac ctggagtaaa
       61 aataattttt acgccaacct caagaagtgc tgggatgtcg tcctggggaa ttactccgcc
      121 gccaaaaacc acaatgtctt cacgctgctt ttctttcaaa agcgagatca cttctggaaa
      181 aacagtctca tgcgcgccag aaagtactga aatgccaatt gccccgactc tgttctcgat
      241 ggcggcttca acaatctgtt taggggtttg gtgcagcccg gtataaaaaa cttcaacgcc
      301 tgcatcttcg agcgcccgaa ctacaacttt tgcaccgcgg tcgtggccat caagtccagc
      361 tttggccacg agtacccgaa cagttttatc gataagggct gaatccttat tactgccagg
      421 gattgaactg tccatattgc ttaaatatag agtcaataaa taaattcggg atatccagta
      481 ggaccgttaa attattggcc aagtgacact tctcacaccg catgatttat cggtattttc
      541 ccaatgattg cagtaattct gccgaaatca atggattaat ccagaaaatg ttatgtcaat
      601 atattcacag acatcacatt tgcgtttcct aaatcagcca atgtcaggta aaaaagaata
      661 ggtaaataaa ggatttttgc gcactgatgc ctatttggtg acagtgcgca aactagcgag
      721 aggtggcatg tgagcgatac ttcacgtggc cgccaccgcg ctggccgtca tagcgctcca
      781 aagaaaaagg cagttaagcc taaatctgtt tccgcattac ttggtctagc tggagtaagt
      841 tccgtcgtgg taactggcgg aagttcgctg ccgacgctaa ataccaacgt tgttaacgaa
      901 tcttcagata ccactactgc tattccggtt gttaagacaa ctgcaattac caataacctc
      961 atcaaccgag ttcaggccgc aagccgtacc aacgagcgcg aaacattagc cgacgcagaa
     1021 gcctcagccc aagcttctac tgttgctgat ccagcaactg acattcttgc agttatcgac
     1081 accacgccga ctttaaagtc cgaggacatc cgtgcgattc aagctttagc tggatcaact
     1141 gaagatttaa ttaccctaat tgatcaggta caagaagatg ccaatcagca agcagccctt
     1201 caggtgcaac aacaagaaat cgcccgggcc gctgctgcag aagcggaagc caaagtagcc
     1261 gaagccatcc gtgaagccga atctaaaaaa caagccgcaa tagttcaagc tcagcgaatt
     1321 gaaacgatcg gaaaagtact agttccagtt caagatggtt accagctttc ggctcgattc
     1381 atgcaacgtg gccgaatttg gtcaggtgga gttcatactg gacttgattt ccgcgttcca
     1441 gttggtactc gtgtttatgc tgctgcttcc ggaactattg ttgaagctgg ctacgaaggt
     1501 gcctacggct accgaattgt cgttgatcat ggtgatggat acatgacctc ttacaaccac
     1561 ctttcaacta tttttgaaac tggtggtttt attactgccg gtacaaatat tggactatct
     1621 ggttctaccg gaaagaccac cgggccacac ttacatcttg aggtgttaaa agatgggcag
     1681 ttcattaacc catctggttg gctttgggga actgattaaa aacttaaatt aaagtttttc
     1741 tactggcgca tatcgcaata acagccgctt tattcccgca ctcttgaaat caatcgtggc
     1801 ttcagctttt tcattttcgc cgcgaacttc cgtcaccgtg cccataccaa atttgtcgtg
     1861 cagtacccga tcgccaacaa tcaaaaccat gacttgttca tcacgtttct ttggtcgctc
     1921 aaagtcgcca cttaaaccaa gtggggtgaa ttcttcgcct tggcgttcta ctaattcatc
     1981 aggtaattca gctaagaatc tcgattctgg attgtaagtt ggttggcccc aagtagacct
     2041 agatgcggct cgacttaagt gcaaacgctg cattgcacgt gtcattccaa cataagccaa
     2101 tcgacgttcc tcttgtagtt ctgtttctga tccaagtgaa cgctgatgcg ggaagattcc
     2161 ttcttccata cctgttaaaa atacaactgg aaactccaaa cctttagctg tatgtaacgt
     2221 catcattgtt acaacgccgc cgtgctcatg gtcttttgga atttcatccg aatcagccac
     2281 taacgaaact ctttctaaga aatctgaaag tctggctttt ttattctctt cattctctgc
     2341 ctgaaattgt tcttcaaatt cacgcgctac attttcaagt tcagatagat tttcgattcg
     2401 cacctcatct tgcggatctt tgctttgggc taactctttc aataatccag aagattcaag
     2461 aactgtatga actatttcac tcggaccagc acctgaatca tccatcattc gaagtaagtt
     2521 aagtaaggca ctgaattctt tcaacgcatt ctgagatctc gctgctaaag tattaatttc
     2581 actaactcga tcgactgctt cgctaaaaga aattttttca cgccgggcaa aattttcaac
     2641 tgcttcttca gctttgtcac ctatgcctct tcgaggagta tttataattc gacgaagtga
     2701 tacgtcatca gtggggttca caattgagcg taaataaccc aacgcatctc taatttcttt
     2761 tctttcataa aaccgagttc caccaacgac tttgtatgga attccattac ggataaatac
     2821 ttcttcgaca cttcgagatt ggctattagt ccggtagaaa actgctacat ctttataggc
     2881 aaatccattc tgatcgatta attcacgaag ttttcgaaca ataaaatctg cttcgtgatg
     2941 ttcatctgca gctgaataga taacgatttt ttcgccatcg ccttgggcag tccataaatt
     3001 ctttttaggt cggccaacat ttttatcgat taccgcattc gctgcagaga gaatgttttg
     3061 ggtggatcga taattttgtt caagtacgac agtggttgat ttggaataat cacgctcaaa
     3121 ttccatgatg tttcttatat ttgcgccacg aaatgcatag atactttggt cagaatcacc
     3181 tacgacacag agttctgcag gttctattcc atcgcttccg ttgccgacca attcccgaat
     3241 taagacatac tgcgcatggt ttgtgtcttg atattcatca accaaaacat gtctgaatcg
     3301 gcggtgatac attgcagcga cttcaggaaa tgcttgaaaa agtgcaacgg tgtgaccaat
     3361 caagtcatca aaatctactg cgctagccat catgagtcgt cgctggtaat caccataaac
     3421 ctgcgcaaca gtttgttctt gagtggtttg cgcctttgtg acagctgtct cgtaatcaat
     3481 taattcattt tttaaattac ttacttggga taacaagtat tttgctgaaa acatttttgg
     3541 gtcgatccca agatctttgg caactaaagt catcaaccga actgaatctt gagtgtcata
     3601 aatactgaaa gtgggtcgta agccaagtcg ctttgcctcg ctgcgcaaaa tacgcacaca
     3661 tgcagagtga aaagttgaaa cccacattgc tcgagcttgc gggccaacta tgtgtgcaac
     3721 ccgttccttc atttcattgg ctgctttatt tgtgaaagtg atagctagga tttcgcctgg
     3781 atgaacattt cgctgcgcaa gcaaatatgc aatccgtcgg gttaaaactc tggttttacc
     3841 ggatcccgca ccggccacca ctaaaagtgg ggaaccagca tgggttaccg cctgacgctg
     3901 aggttcgttt aagtcatcta ataaatgcgc cggcacaggt acgattctac gttcgcgcgg
     3961 tgacacctct agagttgagt acgtgacttt gaatgaccaa aactttgaaa ttgatgagcg
     4021 cttcggtatc cccgagggtg ttgaacgagt tttagtagta actgcgcacc cagatgatgt
     4081 tgactttggc gcaggtggca cgatcgcaac actgaccgaa ttagggattg cagtctcata
     4141 ctgcatttgc accgacggtg atgcgggtgg ttttgatgac gcaacagatc gatctcaaat
     4201 tccgagcatc cgcagagcag agcaaattgc agctggaaaa gttttaggtg taactgacgt
     4261 aaactttctg ggctacaaag atggttattt agaaccaaat catgatgtgc aaaaggacat
     4321 agtccgtatc atgcgaagag taaagccaca attagttatt actcagactc ctgagcgtaa
     4381 ttgggaacgg ctgccagcaa ctcatcctga ccacatggct gctggtgaag cagccactag
     4441 agcgctatac ccagcagttc gaaatcctta tgcgtatcca gaacttcgaa atgaagaggg
     4501 attggaagca tggactgtta gttggttgtg gttacaaggt gactccaacc caaatcattg
     4561 gctggatgtg acttcaaaat tcgagcaaaa aattaaagct attagtgcac acgcaagtca
     4621 agtaagtcac ttagatgatt tagctggcat ggtgcgtgaa tggcagctgc ttgcagcaag
     4681 tcgagccgga ttacccgaag gccaacttgc cgaaggcttc cgtcaaatta aattgccaga
     4741 ttaaaaatta attaaatctg gataatttta aattaaataa atacggcaac gattacgttg
     4801 agcatagtta agccgccaac tgtagcccac atacttttgg cgtcagttgt tgattttctt
     4861 ccaatcaaaa caactaccaa tacagcaagt gacacaccta atttaattcc aatttttgtg
     4921 tggtcaacgg tttcatcagc taacaatcct gctgaaagga ttcctgccaa acctaaacca
     4981 gttactaact gagttaaagc gccatgaaac atggcagggt ttattccttt tgttgcacta
     5041 gacatttgaa ctaagaatcc acccaataag gaagacagac ctacgaggtg cagtacaaca
     5101 agtaagttat aaacaaattc catttttatt cccattcaat tgtgcctggt ggcttactgg
     5161 ttacatctag tacaacacga tttacttcac gaacctcgtt agtaatgcgt gtcgaaatct
     5221 ttgccaataa ttcattaggt aatcgagtcc agtctgcagt cattgcatct tcgctcgata
     5281 caggtcgaag cacaattgga tggccatagg tgcggccatc cccttgaacg ccaacgcttc
     5341 gcacatcagc tagcaacacg actgggaact gccaaatact gctatttaat ccagctaaag
     5401 taacttcttc tcgaacaatt gcgtctgcgg aacgcaaaat atccaaacgt tctttattaa
     5461 cacttccaac tattcgaata cctagaccag gtccagggaa tggatgacgc ataactattt
     5521 cttcgggtag tccaagttgg cgtccaactg cacgtacctc atctttaaat aattcgcgga
     5581 ggggttcaca aagttcaaaa tctaaatcat ctggtagtcc accaacattg tgatgtgact
     5641 taattgtggc tgccccagtt ccgccaccac tttctacaac gtcgggatac aaagttcctt
     5701 ggaccagaaa cttaattgga tttccggcat tacctgcttg ggtaactaga tctcgttgta
     5761 catcttcaaa tactcgaata aacaatcgac caattatttt tcgcttttct tccgggtcag
     5821 taactccctc gagttcagat agaaatcgct gcgctgcatc aaccaccact aatttaatgc
     5881 cagtagctgc aacataatca cgttctactt gttcacgctc tcccaaacgc agtaagccat
     5941 ggtcaacaaa cacgcaggtt aaattatcgc caacggcctt gtgcactaac gctgcagcta
     6001 cggctgaatc aacaccgcca gatagaccac aaataacttg atgatcgcca atttgttttt
     6061 ttattcgagc aacttgttca tcgactattc cggctggggt ccagtctggt tttatgccag
     6121 caatttcgta aaggaaatgt tctaaaactt tttgaccata ctcagagtgc atgacttcag
     6181 gatgaaattg aacaccggca aatttttgag ttaagttttc aaaagctgcg actggcgcgc
     6241 cagcgctcga tgctgttacc tcaaaaccat caggggcaac ttggacactg tcaccgtgac
     6301 tcatccaaac gttttgtgac ttaggcaagc cagcaaataa acaccccggt tttgtgaccg
     6361 ttaattcagt tgcaccaaac tccgataaac ctgtttttgt aacctgtccc ccgagtgcac
     6421 ttgccatggc ttgaaagcca tagcaaattc caaacactgg tagcccagtt tcaaataatt
     6481 ttgaatctac ttgcggagca cccgcggcat aaacacttga aggtcctcca gaaagcacaa
     6541 tagctaacgg attcttaaga agtatgtctg caactgacat agtgcttgga actacttcgg
     6601 aatagatgtt cgattcacga att
//
LOCUS       DBNHVN010000083         1043 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1204337, whole genome shotgun sequence.
ACCESSION   DBNHVN010000083 DBNHVN010000000
VERSION     DBNHVN010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1043
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1204337"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..>1043
                     /locus_tag="AC16Y1_01540"
     CDS             46..>1043
                     /locus_tag="AC16Y1_01540"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HIR5831180.1"
                     /translation="MSTTQRLVIVESPAKAKTIQKYLGPGYEVEASVGHVRDLPERAV
                     DVPSEIKKQPWGRMAIDVEDDFTPYYVVSSKKKDKVAELKRKLAEADELLLATDEDRE
                     GEAIAWHLMEILRPKVPVKRMVFHEITPEAIKHAVETTRDLDMQMVDAQEARRLIDRL
                     YGFEVSPVLWRKVQAGLSAGRVQSVATRLIVERERERIAFKSASYWDIEAVFDPGTFG
                     ATLVQVDGQKVATGKDFDSLAKLTTKNDVIHLNEAEATSLAENLANVKFEIRKVDEKG
                     YTNRPKAPFMTSTLQQAASGKLKWGAQRTMRVAQGLYERGYITYMRTDSTTLSETA"
BASE COUNT          305 a          231 c          250 g          257 t
ORIGIN      
        1 cggtccgccg agaagagcct tctgtcacac gcgaaagggt aaaggttgtc tacaacacaa
       61 cgtttagtga ttgttgaatc cccggcaaaa gccaaaacaa ttcaaaaata tttaggtcca
      121 ggttacgaag ttgaagcttc ggttggtcac gttcgcgatt tacccgagcg ggcagtcgat
      181 gtgccgtctg aaattaaaaa gcaaccttgg ggccgaatgg ccattgatgt tgaagatgat
      241 ttcactcctt attacgtggt tagctcaaag aagaaagaca aagtcgctga acttaagcgc
      301 aaattggccg aagctgacga actgctactt gccactgacg aagaccgtga aggtgaggct
      361 attgcctggc acctgatgga aatccttcga ccgaaagttc cggtcaaacg aatggttttc
      421 catgaaatca ctccagaggc aatcaagcac gccgttgaga caactcgcga tttagatatg
      481 cagatggtcg atgcccaaga agcccgacgc ttaattgacc gactttatgg ctttgaggtt
      541 tccccagttt tatggcgcaa agtacaagct ggcctttcgg ctgggcgcgt gcaatctgtg
      601 gcaacccgat taatcgttga acgcgagcgc gagcgcattg ctttcaagag tgcttcgtac
      661 tgggatatcg aagcagtttt cgatccagga acttttggcg caactttagt tcaagttgat
      721 ggccagaaag ttgcaactgg taaagatttt gattcgcttg ccaaactaac tacaaaaaat
      781 gatgtcattc atttaaacga agcagaagca acatcgcttg ccgaaaattt agcaaacgta
      841 aaatttgaaa tccgtaaagt tgatgaaaag ggttatacaa atcgacctaa agcaccgttc
      901 atgacttcaa ctttgcagca ggctgcctcc ggaaaattaa agtggggtgc gcaacgcact
      961 atgcgcgttg ctcaaggttt atacgaacgc ggttacataa cttatatgcg tactgacagc
     1021 acaacacttt ctgaaactgc aat
//
LOCUS       DBNHVN010000084         2930 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_312555, whole genome shotgun sequence.
ACCESSION   DBNHVN010000084 DBNHVN010000000
VERSION     DBNHVN010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_312555"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="AC16Y1_01545"
     CDS             complement(<1..533)
                     /locus_tag="AC16Y1_01545"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5831181.1"
                     /translation="MIKKVAVDLTLLKTNRNFRNLWLSSLITRFGSMITYVAAPYQIK
                     ELTGSYIAVGLIGVFQIIPLIIFGLYGGSIADRYDRKKVVLWCELGFLILVSALMLNS
                     LSANPSILLIYFVGMTMAALDGIQRPSLTAMMPRLVSREQLPAAGALDSITHNAGYLV
                     GTSVGGLLVVSVGLSWS"
     gene            complement(632..1462)
                     /gene="panB"
                     /locus_tag="AC16Y1_01550"
     CDS             complement(632..1462)
                     /gene="panB"
                     /locus_tag="AC16Y1_01550"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017546722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR5831182.1"
                     /translation="MSSQTLYGGMQSRRITTSDLISAKSRGEKWPMITSYDAITARIF
                     DEAGYPVLLVGDSAAMVVYGFDSTIPVTSDDLIPLVAAVHRGSKRAMVVADLPFGTYQ
                     ETPQLALNNALRFMKEGNAHAVKIEGGAAMVPTVELLTKSGIPVMGHIGLLPQSVHQM
                     GGYRVQGRGDEGKKLIADAIALEAAGAFSIVLEVIPSDLAQQITAAVKIPTIGIGAGP
                     HTDAQVIVWQDLLGLNSGKPAKFVKQYANLAKDISQASKAWAQDVISGAYPDAAHSYE
                     "
     gene            complement(1529..>2930)
                     /locus_tag="AC16Y1_01555"
     CDS             complement(1529..>2930)
                     /locus_tag="AC16Y1_01555"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003960282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIR5831183.1"
                     /translation="AAVIHQGKILGHYNKHHLPNYGVFDEFRHFVSGDESLVIRAFDT
                     DIALAICEDLWQDGGPVARVQDSGASLLLVLNGSPYERNKDDVRLELVKKRAAQANAT
                     LAYVNMVGGQDDLVFDGDSIVVSPQGKVLARANQFQDELLVLDVEVRPASSMTKLPTG
                     EQVLAIDQKPAVKPETLSQTVRKQLSDYEELYSALVLSLHDYVHKNGFKSVIFGLSGG
                     IDSALVAAIACDAIGAKNVYAVSMPSKYSSEHSKTDAQDLASRTGLNFREIAIEPMVT
                     SFLSELKLSGLAEENLQARVRGMTLMGISNQEGHLVLATGNKSELAVGYSTIYGDAVG
                     GFAPIMDVPKVDVWALANWRNEKALSQGLTAPIPESSITKPPSAELRPGQKDSDSLPD
                     YEVLDAILVEYIEFDRSLDEIVALGYPEEVVQKTLQLTDRAEYKRRQYPPGPKVSGKA
                     FGRDRRLPMTSKWRES"
BASE COUNT          807 a          685 c          646 g          792 t
ORIGIN      
        1 tatgaccagc tcaacccaac gctgactaca agtaagccac caacagaggt accgactaaa
       61 taaccagcat tgtgtgtgat cgaatctaag gcgccggcag ccggaagttg ctcacgacta
      121 actagtcttg gcatcatcgc ggttagtgat gggcgctgaa taccgtctag cgcggccatg
      181 gtcatgccaa caaaataaat aagcaatatc gatgggttag cagaaagtga attcagcatc
      241 agtgcactaa ctaaaatcaa aaatccaagt tcgcaccaaa ggacaacttt ttttcgatcg
      301 taacggtcgg cgattgagcc cccgtaaagc ccaaaaatga ttagtggaat tatctgaaat
      361 acaccgatta atccgactgc tatgtatgag ccagttagtt cttttatttg atacggggct
      421 gcgacatagg taatcatcga accaaaacgg gtgattaatg aactaagcca taaatttcta
      481 aaatttcgat tagttttaag tagcgttaaa tcgactgcaa cctttttgat cactgttcac
      541 ccgaatcttt aagattcaac aggttagcca gacaggtaaa ttgcactgac tcagtttgcc
      601 acagtattta tcagggttta attaacggtt gtcactcata agagtgggcc gcatctgggt
      661 aggcaccact aataacgtct tgtgcccaag ctttacttgc ttgactaatg tccttggcca
      721 gattggcata ttgtttaaca aacttggctg gcttacccga gtttagcccg agtaaatctt
      781 gccaaacaat aacttgcgca tcagtatgtg gcccagcacc aatcccaata gttggaattt
      841 ttactgccgc tgtgatctgc tgtgctaagt cacttggaat tacttcgagc actattgaaa
      901 aagctccagc tgcttcaagt gcaattgcat cggcaattaa ttttttacct tcgtcgcctc
      961 ggccttgaac gcgataacct cccatttgat gaacagattg ggggagtaaa ccaatatgcc
     1021 ccatcactgg aattccagat ttggttagga gttcaacagt aggcaccatg gcagcgccac
     1081 cttcaatttt taccgcgtgc gcattacctt ccttcataaa acgtaaagcg ttattcaggg
     1141 ctagttgtgg agtttcctgg taagtaccaa atggtaaatc agctacgacc atcgcacgtt
     1201 tacttccgcg atgcacagca gcaacaagcg gaatcaaatc gtcactagtt actggaatgg
     1261 tcgagtcgaa gccatagacc accatggccg ccgaatcacc gactaaaaga acggggtacc
     1321 cggcctcatc aaaaattcga gctgtaattg cgtcataact ggtaatcatc ggccactttt
     1381 caccacgaga ttttgccgaa attagatcac tagttgtaat gcgcctagat tgcattccac
     1441 cgtaaagggt ttggctagac atgttggtac tccgatctcg aggccagcaa gtggtccccg
     1501 gatatgcact tccaactatt tcaggtaatt aagattcgcg ccatttgctg gtcattggta
     1561 aacgccgatc gcggccaaat gccttgccag aaactttggg gccaggcgga tattggcggc
     1621 gcttgtattc agctcgatcg gttaattgca atgttttttg aactacttct tctgggtaac
     1681 ccagcgccac aatttcatct aggctgcgat caaattcgat gtactcaacc aagatggcat
     1741 caagaacttc atagtccgga agtgagtcag aatctttttg gccagggcgc agttcagcac
     1801 taggcggctt tgtgattgag gattctggaa tgggcgcagt taaaccttgg ctaagtgctt
     1861 tttcattgcg ccaattggct agcgcccaga catctacttt aggtacatcc ataatcgggg
     1921 caaagccgcc aaccgcgtca ccgtagatag ttgaataacc aaccgctaat tcacttttat
     1981 ttccggtggc taaaactaag tggccttctt gatttgaaat acccatcagg gtcataccgc
     2041 gaactcgagc ttgtaagttt tcttcagcta atccagataa tttcagttca gataaaaatg
     2101 aagtgaccat gggttcaatg gcaatctctc ggaaattaag tccagtgcga ctggcaagat
     2161 cttgggcatc ggtttttgag tgttccgatg agtattttga tggcatcgaa acggcataaa
     2221 cattcttagc tccgattgca tcacatgcga tggcggcaac taacgcggag tcaatacccc
     2281 cggataagcc aaaaattaca gacttgaaac catttttatg tacgtaatcg tgcaaactca
     2341 gaactaaggc gctgtacaac tcttcgtaat cagatagttg cttacgcaca gtttgagaaa
     2401 gtgtttcagg ctttactgcg ggtttctgat ctattgccaa aacttgttcg ccagtaggca
     2461 acttagtcat ggaactggct ggtcttacct cgacatcaag cacgagtaac tcgtcttgga
     2521 actgatttgc cctggctaat actttgcctt gtggcgaaac cacgattgag tcgccatcaa
     2581 aaactaaatc gtcttggccg ccgaccatgt ttacataagc caaagttgca tttgcctgtg
     2641 ctgctcgttt tttaactaac tcaaggcgaa cgtcatcttt attgcgttcg taaggtgacc
     2701 cattcaggac aagcaaaagg ctggcaccac tatcttgaac tcgggccacc ggtccgccgt
     2761 cttgccataa gtcttcacag atagccagag caatgtcagt atcaaaagct cgaattacta
     2821 gggattcatc gccggaaaca aaatggcgaa actcatcgaa aactccatag tttggtaagt
     2881 gatgcttgtt gtagtgacca agtatttttc cttggtgaat tacggcagcg
//
LOCUS       DBNHVN010000085         2631 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_15470, whole genome shotgun sequence.
ACCESSION   DBNHVN010000085 DBNHVN010000000
VERSION     DBNHVN010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2631)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2631)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2631
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_15470"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..925
                     /locus_tag="AC16Y1_01560"
     CDS             <1..925
                     /locus_tag="AC16Y1_01560"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5831184.1"
                     /translation="TVGPLSGVNVTTGRNFQSSDVTSTNAIVDSTYAAAQKILVGATF
                     DVGGKPFTVIGIVASSSASPETASNVYIPLSQAQTLSGSTGLVSNIYVSADSSSNTGN
                     IKKSIESLLPDATVSSQADLAQNLSGSLSSASSLVNALSRWLSIIVLFVAFLIAILFT
                     TSGVTRRIRELGTLKAMGWKTNRIVRQIMGESLVTSFIGAFIGLIMGVVGIMVVNAVA
                     PTLSAAVARPGRFAGGGGFTPPTGGFGGRGTGFGQTAANATTLKLHIGMNISTLIVAV
                     AIAIIGGLIAGSFGSWRVSRLSPAVALRSAA"
     gene            925..1608
                     /locus_tag="AC16Y1_01565"
     CDS             925..1608
                     /locus_tag="AC16Y1_01565"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010984750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5831185.1"
                     /translation="MTNSVLYSLKDLSKSYSQNNKNVDALKKVNLEINRGDLISIQGP
                     TGGGKSTLLQMLGGLDKPTSGSISLSDVNLETLSESKLTEIRARKIGFVFQNYNLIPT
                     LSALENVQTALVPQGVSKADSEKRALAALESVSLADRATHLPAELSGGQQQRVAIARA
                     LVKNPEVILADEPTGNLDEQTRDQIIDLLVDLNKNQGITIILVTHDSSVAKKANKNLH
                     IGNGVVTTR"
     gene            complement(1815..2243)
                     /locus_tag="AC16Y1_01570"
     CDS             complement(1815..2243)
                     /locus_tag="AC16Y1_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831186.1"
                     /translation="MGKNHPIAMINSVSNSKHLGCPSCAIGGFDPNLKGYFYFLSHPS
                     WEMFQIGITNYPEDRLQKHRKLGWELLEIRGPMDGHLTQQWETAVLRMLKAKGADLSN
                     SNMAGKFDGYSEAWTKSTFEAISILQLMDLTEEFESSKKT"
     gene            complement(2394..>2631)
                     /locus_tag="AC16Y1_01575"
     CDS             complement(2394..>2631)
                     /locus_tag="AC16Y1_01575"
                     /inference="COORDINATES: protein motif:HMM:NF016150.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HIR5831187.1"
                     /translation="MQLSFPGRMSLTNYILQSLALSLIYGPWGLGLFQKIEFWNSFLI
                     AILVWFSLLLISKFWLTKFSKGPAEWLLGRLSQK"
BASE COUNT          762 a          520 c          587 g          762 t
ORIGIN      
        1 aactgttggt ccccttagcg gcgtgaatgt gactactggc cgaaattttc aatcatctga
       61 tgtgacttca acaaatgcaa tagtggattc aacatatgct gcggctcaaa aaattttggt
      121 cggtgcaact tttgatgtcg gcggaaaacc gtttactgta attggaattg tggcttcatc
      181 atcagcttca ccagaaacgg cttcaaatgt ttatatccct ttaagtcagg ctcaaacttt
      241 gtctggtagc acgggtttag tttcaaatat ttatgtatct gctgattcct cttcaaatac
      301 tggcaatatt aaaaaaagta ttgaatcatt attacctgat gcaacggttt catcgcaagc
      361 tgatttggca cagaaccttt caggttcgct tagttctgca tcttcacttg tgaatgcact
      421 tagtcggtgg ctctcaatta ttgttttatt cgttgcattt ttaatagcaa tactctttac
      481 aacatcaggc gttactcgcc gaatacgtga attaggaact ttaaaggcaa tgggctggaa
      541 aaccaatcga attgtgcgcc aaattatggg tgaatcactg gtgacaagtt tcattggtgc
      601 atttatagga ttaattatgg gagtagttgg cattatggtt gttaacgcag ttgctccaac
      661 actttctgca gctgttgcaa gacctggaag atttgctggt ggtggcgggt ttactccacc
      721 aactggtggt ttcggcggtc gtggcactgg atttggacaa actgcggcta atgcaacaac
      781 tttaaaactc catataggta tgaatatttc aacattaata gttgctgtag caattgcgat
      841 tattggcgga ttaatcgcag gttcatttgg aagttggcgg gtatcacgat tgagccccgc
      901 tgtggcatta aggagcgcgg cataatgaca aattcagttt tatattcgct caaagatctt
      961 agtaagagtt acagccagaa caataaaaat gttgatgcgc taaagaaagt gaacctagag
     1021 ataaatcgtg gagacttaat ctcgattcaa ggtccaactg gcggtggaaa gtcgacctta
     1081 cttcagatgt taggtgggtt agataaacct acctctggaa gcatttcttt atctgatgtt
     1141 aatttagaga ctctcagtga atctaaatta actgaaatac gtgccagaaa aattggtttt
     1201 gtttttcaga attacaactt aattccaacc ttatctgcac ttgaaaatgt ccaaacagca
     1261 ttagtcccgc agggagtcag caaggctgac agtgaaaagc gagcattggc tgcattggaa
     1321 tcagtttccc ttgccgatcg tgcgactcat ctacctgctg aactgtcagg tgggcagcag
     1381 caacgtgttg caattgcacg cgcgctggta aagaaccctg aagttatttt ggctgatgag
     1441 ccaaccggaa accttgatga acaaaccagg gaccagatta ttgatctact tgttgaccta
     1501 aataagaatc aaggaatcac gattattttg gtaacacacg attcaagtgt ggcaaaaaag
     1561 gccaacaaga accttcacat tggaaatgga gttgtgacta ctcgttaaga atccaaaagg
     1621 cggtgtcttt taggagagcc cgctgcgaag atccggctta tagggcgact cttcttcaat
     1681 tacatcgctg ccatgctcaa agaagttgaa attgagcgtg gcagctttgt taatttttcc
     1741 caaaacgtaa taagaaaatc taactttttg gggtgcattt gagggcactg ggggcaagaa
     1801 ttggggcgaa gatcttaagt ttttttcgag ctctcaaatt cttcagtcag atccattaac
     1861 tgcaaaattg atattgcttc aaaggttgat ttagtccacg cttcagaata accatcaaat
     1921 tttcctgcca tattgctgtt agataagtcg gcaccttttg ccttaagcat tctcaaaact
     1981 gcggtttccc actgttgcgt aagatgtccg tccattggac ctctgatttc caataactcc
     2041 cagcctagtt ttcgatgctt ctgcaggcga tcttccgggt agtttgttat tccaatttgg
     2101 aacatttccc aagatggatg cgacaagaaa tagaaataac ctttgaggtt cggatcgaag
     2161 ccaccaattg cgcacgatgg gcaaccgagg tgtttgctgt ttgatactga attaatcatg
     2221 gcgattggat gattcttgcc caaggcctca agctccttga gaagttagcc aaaattgacc
     2281 caacgattca gcccgaagtc aaagctgtag cgagaaggct tcaaaaagat actaggaaag
     2341 ccgttgctac taaggccaag aaagttctat ccggcttata gggcgactct tctttatttt
     2401 tgcgaaaggc gaccaagtaa ccattccgct gggccttttg aaaacttggt gagccagaac
     2461 tttgaaatca ataaaaggga aaaccaaacc agaattgcaa ttaagaaaga attccagaat
     2521 tcaattttct ggaataggcc aaggccccaa ggaccataaa ttagagataa agctagagat
     2581 tgtaaaatgt aattagtgag cgacattcgc ccagggaaac tgagttgcat t
//
LOCUS       DBNHVN010000086         7320 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1650277, whole genome shotgun sequence.
ACCESSION   DBNHVN010000086 DBNHVN010000000
VERSION     DBNHVN010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7320)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1650277"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /gene="tig"
                     /locus_tag="AC16Y1_01580"
     CDS             complement(<1..1183)
                     /gene="tig"
                     /locus_tag="AC16Y1_01580"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR5831188.1"
                     /translation="MKSAVENLSPTRVKLTVEVPFADLSESMEAAYARIAKSVNIPGF
                     RKGKIPPRIIDQRFGRAAVLEEAVNDAIPSSLAKALIENKIAQLGQPEVDVTKLDETE
                     GLAFTAELDIRPEFDLPKFEDLKVVVDDAVVTEADIDEQLKELRARFGSLKAVERPAA
                     DGDIVMIDLRGTHDGEEVEDIAANAMSYELGSDGMVPGFDDAVRGASTGDERSFVFTP
                     TNGAWTDKTIEVAVSVRSVRERELPEANDEFAQLASEFDTIADLRNDIKDRLGRLRLV
                     EQAYQARDLTHDALMEAIKVEIPQGVVKAEIDAHFADGHGDEAHKTEFEQNTRSGVKS
                     RMILDKIAEEQEVQVNDAELSQWLISEAQRYGMAPDQFADQLVQSGQVQGAIAEVRRV
                     KA"
     gene            complement(1232..1307)
                     /locus_tag="AC16Y1_01585"
     tRNA            complement(1232..1307)
                     /locus_tag="AC16Y1_01585"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1272..1274),aa:Pro,seq:tgg)
     gene            1377..1450
                     /locus_tag="AC16Y1_01590"
     tRNA            1377..1450
                     /locus_tag="AC16Y1_01590"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1409..1411,aa:Gly,seq:tcc)
     gene            complement(1588..2301)
                     /locus_tag="AC16Y1_01595"
     CDS             complement(1588..2301)
                     /locus_tag="AC16Y1_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019120 - hydrolase activity, acting on
                     halide bonds, in C-halide compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HIR5831189.1"
                     /translation="MSEFSKFSSLSFDCYGTLIDWESGIANELLEWTAKNNFQVSREQ
                     LLVDFSDYEAAAERDFPDLLYPKVLAKAMEGLGSHYGITATAEDLASFGESVPRWPAF
                     PDSAEALKILKQKFKLIILSNVDRNSFAASNKKLEVEFDAVVTAQDVGAYKPRLDGFH
                     LLAKTVKDLGVPEGGLLHVAQSLFHDHVPAKQVGLSTVWINRRHNDPGWGATPAPSAD
                     VKPDWTFNSMIEFAKAATA"
     gene            2382..4922
                     /gene="pepN"
                     /locus_tag="AC16Y1_01600"
     CDS             2382..4922
                     /gene="pepN"
                     /locus_tag="AC16Y1_01600"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HIR5831190.1"
                     /translation="MSGTNITRQEAQERSAVVTAKSYEIELDLSTRTDTFPSKTTLEF
                     SAREGNSTWVDYIAPKVNQIELNGESIDLAAHDGYRIRLQNLKSHNTLVVVGESKYMN
                     TGEGLHRFIDPVDDEVYLYTQFEIADARRVFSCFDQPDQKATFKFTITAPDHWKVQSN
                     SPTPDPKAVSKDVVKYEFVPTHLMSTYITAIVAGPYHEVRDVYEGKFGSYPLGFFCRK
                     SLADYLDPDDLFEVTKQGFKYFEEAFDYGYPFGKYDQLFVPEFNAGAMENAGCVTHHE
                     DYVFRSKVTRASYEQRANTILHELAHMWFGDLVTMKWWDDLWLNESFAEWAAHHASVK
                     ATQYTEAWTNFVNQRKAWGYRQDQLPTTHPIVADMVDLDAVEVNFDGITYAKGASALR
                     QLVAWVGEDEFFAGIRSYFKKYAWGNTELPDLLKELEVASGRDLNDWTTRWLQTAGVN
                     TLRPEVVIENGVYKSVVVLQEPPVAPAGVEQLLRPHRIGVGIYQKSGQTIERVNVVEL
                     DVDGPRTEVTELVGKPAGDLLLLNDGDFTFVKIRLDELSAKTAAQNLDGVTDSVSRAL
                     IWAAAWDMTRDAEMPTGDYLNMIMGANLVDMGIGVSQQLLLQARSAIEQFGAPNKRHI
                     YRDQMIAALLTQLPSAPAGGDHQLSFVRNIIALTRTPEHIELIKSWLTGTNTPNGLAI
                     DTDLRWAFISRLASLGALPANAIEEELARDNTAGGQRQAASARAALPTAEAKAQAWAS
                     VVDSDDLPNAILDATVGGFAQPDQRELLSPYVEKYFAALTEIWKSRTNEIAQSLTTGL
                     FPSLLASEDILKRADAFLATDKDAGSTRLIKELRDNVARSLRCQAKDS"
     gene            complement(4931..5167)
                     /locus_tag="AC16Y1_01605"
     CDS             complement(4931..5167)
                     /locus_tag="AC16Y1_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831191.1"
                     /translation="MRLRRAFAITTSTLVLSASFVAPAIANKRDDGDEPGSVLNASTA
                     IVLFGLIPLAITALITLFVLAPGWTKKSKDLTKY"
     gene            5271..5825
                     /locus_tag="AC16Y1_01610"
     CDS             5271..5825
                     /locus_tag="AC16Y1_01610"
                     /inference="COORDINATES: protein motif:HMM:NF045289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR5831192.1"
                     /translation="MPDLFEWFLGTPVKIAVIILLAFLLRVVLIRASSRVIKRALLIR
                     RDRAKQSGNAQNESAAQDRYEQRARSISALLRSVISVTVWSLAAIMALSNLGIDVAPI
                     LASAGVVGVALGFGAQTLIKDFLAGIFMILEDQYGVGDLIDVGPAIGTVEEIGLRVTR
                     LRDESGIVWYMRNGEVLRIANHSQ"
     gene            5830..6213
                     /locus_tag="AC16Y1_01615"
     CDS             5830..6213
                     /locus_tag="AC16Y1_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016435104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HIR5831193.1"
                     /translation="MMETTLYEQMGGTPFFEKLVIGFYEGIKSDPVLKPMYPEEDFAG
                     ATRRLTMFLSQYWGGPTDYSQERGHPRLRMRHNVFPINSDARDRWLTHMSNSLAKLEI
                     EPHLKDQLWTYLVTAAHSLVNLPDN"
     gene            6258..>7320
                     /locus_tag="AC16Y1_01620"
     CDS             6258..>7320
                     /locus_tag="AC16Y1_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012356.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR5831194.1"
                     /translation="MLRAKDWWREAVVYQIYPRSYADASNDGIGDIPGIRSKVSYLKS
                     LGIDAVWLSPHYPSPMLDAGYDVSDYRDVEPIFGKLEDIEGLIKDLHAAGLKMFIDIV
                     PNHTSWEHAWFKEALANPPENDPAIPAKARYAEGPWAKYHLLRGQNDGKSEPNNWESV
                     FGGPAWHEVKDLLGKPTGWWYLHIFDKSQPDLDWDNQEVRADFRQTLRFWFEKGIDGF
                     RIDVAHGIAKADGYPDCPEILDGDPTNKNPYWDQDALHEVWREWRKVADEFEPQRVFV
                     AEAWVFPAERNARYLRPDELHTGFNFPFLKCPWEAEKLRETIDMSMTANAIAQAPTTW
                     VLENHDVWRAATRYAPVIGS"
BASE COUNT         2025 a         1640 c         1643 g         2012 t
ORIGIN      
        1 aagctttcac tcgacgtact tcagcaattg caccttggac ctggccagat tgcacaagtt
       61 gatcagcgaa ttgatcaggt gccatgccat aacgctgggc ttcactgatt aaccactgtg
      121 agagttctgc gtcattaact tgaacttctt gctcttcggc aattttgtcc aaaatcattc
      181 gactttttac tccggaacgt gtgttttgtt caaactcagt tttgtgtgcc tcgtcgccat
      241 gaccgtcagc aaagtgtgca tcaatttctg ctttaactac accttgagga atttcaactt
      301 taattgcttc catcaaagca tcatgcgtta aatcacgagc ttggtatgcc tgctcgacaa
      361 gtcgtaagcg acccaaacga tctttaatgt cgttgcgtag atcggcaatg gtatcgaatt
      421 cacttgcaag ctgagcaaat tcatcgtttg cctctggcag ttcgcgctca cgcactgagc
      481 gaactgatac tgctacttca attgttttgt ctgtccaagc accgttagtt ggagtaaaaa
      541 caaatgaacg ttcgtcgcca gtagaagcgc cgcgaactgc atcatcgaag cctggaacca
      601 tgccgtctga gccaagttca tagctcatgg catttgctgc aatatcttca acttcttcac
      661 catcatgtgt gccacgaagg tcgatcatca caatgtcgcc gtcagcagca gggcgttcga
      721 cagcttttag cgagccaaat cttgctcgca attctttaag ttgctcatct atatcagcct
      781 cagttacgac agcgtcatcg acaacaactt tgaggtcttc aaactttggc aaatcaaact
      841 caggacgaat atctagttca gcagtgaaag ctaatccttc tgtttcgtct aatttagtga
      901 cgtcaacttc aggctggcca agttgggcaa ttttgttttc aattaaagcc tttgccagtg
      961 aacttgggat tgcgtcattt accgcttcct ctagtactgc agctcgacca aaacgttggt
     1021 cgataattcg tggtggaatt tttcccttac gaaagccagg aatgtttacc gattttgcaa
     1081 tccgtgcgta ggccgcttcc atactttcgc ttaaatccgc gaatggcact tcaaccgtaa
     1141 gcttgacgcg ggttggggaa agattttcaa ctgcgctttt cacggttctc ctatttattc
     1201 actcactggc cacttaattt ttaaaaagtt atggtcgggg cggagagact cgaactctcg
     1261 gtcttctgct cccaaagcag acgcgctagc cactacgcta cgccccgacg acctgatgaa
     1321 gtctattcca attaatgcga taatctaaat tcagatttgc ttaagggcga atttacgcgg
     1381 gtgtagctca atggtagagc cccagccttc caagctggcc atgccggttc gatcccggtc
     1441 acccgctcca aatcgctagc gcgatttgga gcatcgcaca gtacctattt ggtttggctc
     1501 cattcgtcgc caaacttaac caactccatt accatcgctt cgcgctgaga gcagatcccg
     1561 tgtgaccggg atcgaacctc aaatcattta tgcagtagca gctttagcaa attcgatcat
     1621 cgagttaaaa gtccaatcag gtttcacatc agccgatggt gcaggcgtgg ctccccagcc
     1681 tggatcatta tgtcgacgat ttatccaaac ggtcgacaaa ccaacttgtt ttgctggcac
     1741 atgatcatgg aacaaactct gggccacatg aagtaaacca ccttcaggaa cgcctaagtc
     1801 ttttactgtc ttagccaaca aatgaaaacc atctaagcgt ggcttatacg cgccaacatc
     1861 ttgagctgta acaaccgcat caaattcgac ttcaagcttt ttgttactag ccgcaaatga
     1921 gtttcgatca acatttgaaa gaataattaa tttaaatttc tgtttgagga ttttgagcgc
     1981 ttcagctgaa tcaggaaatg ctggccagcg tggcacagat tcaccaaaac ttgctagatc
     2041 ttcggcagtc gccgtaatcc cgtaatggct acccaaacct tccattgctt tagccaaaac
     2101 ttttggataa agcagatctg gaaagtcgcg ctcagcggct gcttcataat cagaaaagtc
     2161 aacgagtaac tgttcgcggc taacttgaaa attattcttt gccgtccact ccagtaactc
     2221 attagcgatg ccagattccc aatcaattaa ggtgccgtag caatcgaaac ttaggcttga
     2281 aaactttgaa aactcactca tgccttgagc ctatgacccg atttcttatc tgtcacatac
     2341 aggtgtcagg atgtacttct gaagttacta aaggagtact tttgtccggc acaaacataa
     2401 ctcgccaaga agctcaagaa cgttctgcgg ttgttaccgc gaagtcgtac gaaatcgagc
     2461 ttgatttaag tactcgaact gacacttttc cgagcaaaac tacacttgaa ttctcggcgc
     2521 gtgagggtaa ctcaacctgg gtcgattaca ttgcaccaaa agttaatcag atcgaactaa
     2581 atggcgaatc aatcgaccta gccgcacatg atggctatcg aattcggtta caaaatctaa
     2641 agtcgcataa cactttggta gttgtcggtg aatcaaagta catgaatact ggcgagggtt
     2701 tacaccgctt tatcgatcca gttgatgacg aggtatatct ttatacccaa tttgaaattg
     2761 ctgacgcccg acgagttttt tcttgttttg atcaacctga tcagaaagcc acatttaaat
     2821 tcactattac cgcgccagat cattggaaag ttcaaagtaa tagtcccaca cccgacccta
     2881 aagctgtatc taaggatgta gttaaatacg aatttgttcc aacacatttg atgtctactt
     2941 acatcaccgc aatagttgct ggaccgtatc acgaagtgcg tgatgtttat gaaggtaaat
     3001 tcggctcata cccacttggc tttttttgtc gcaagtcact agctgactat ttagatccag
     3061 atgacttatt cgaagtcaca aaacaaggct ttaagtattt tgaagaagcc tttgattacg
     3121 gttatccgtt tggtaagtac gaccaattat ttgttccaga atttaatgct ggggcgatgg
     3181 aaaacgctgg ctgcgtcacc caccacgaag actacgtatt ccgttcaaaa gtaactcgtg
     3241 catcttacga acaacgagca aacacaattt tgcatgaact tgcacacatg tggtttggcg
     3301 atttagtcac catgaaatgg tgggatgacc tttggctaaa tgaaagtttt gccgaatggg
     3361 cagctcatca cgccagtgtg aaagcgactc aatacaccga ggcttggact aactttgtaa
     3421 atcaacgcaa agcttggggt taccgccaag atcaattgcc gactacgcat ccgattgttg
     3481 cggatatggt tgatcttgat gcggttgaag taaattttga tggcatcaca tatgccaaag
     3541 gtgcatctgc actacgccaa ttagttgcct gggttggcga agatgaattc ttcgctggca
     3601 tccggagtta cttcaaaaaa tatgcctggg gtaacactga actacctgac ttattgaaag
     3661 aacttgaagt tgcaagtggt cgtgatttaa atgactggac tactcgctgg ttacaaactg
     3721 ctggcgtaaa tactttgcgg ccagaagttg tgattgaaaa tggtgtctac aaatcagtcg
     3781 tggtcctaca agaaccgcct gtggcgccag ctggggttga gcaacttcta cgtccacatc
     3841 gcatcggcgt tggaatttat caaaaatctg gacaaacaat tgagcgagtt aacgttgttg
     3901 agttagatgt tgatggcccg cgaaccgaag taacagaact agttggtaaa ccagcaggtg
     3961 acttactact tctaaatgat ggagacttca ctttcgtaaa aattcgcctt gatgaattgt
     4021 ctgctaaaac ggccgcacaa aatttagacg gtgtgacaga tagcgtttct cgagctttga
     4081 tttgggccgc tgcctgggat atgacccgag acgcagaaat gccaaccggc gattacttaa
     4141 acatgattat gggtgcgaat ttagttgata tgggaattgg cgtttcccag caattacttt
     4201 tgcaagcgcg atctgcaatt gaacaattcg gggcaccaaa taagcgtcac atctatcgtg
     4261 atcaaatgat cgctgcattg ttgacgcaac ttccgagcgc accagctggt ggcgatcatc
     4321 aattgtcgtt cgtgcgaaac attattgcgt taactcgaac accagagcac attgagttga
     4381 ttaagtcttg gttaactggg acaaatactc ctaatggctt agctatagat acagacttgc
     4441 gttgggcgtt tatttctcgc ctggcgtcat tgggtgcatt gccagcaaat gcgattgaag
     4501 aagaattagc tcgtgacaat actgctggcg gtcaacgtca ggctgcatct gctcgagctg
     4561 cgttaccaac tgcagaagca aaagcacaag cttgggcatc agtcgttgat tcagatgact
     4621 tacctaacgc aattttggat gcaactgtcg ggggcttcgc ccaacctgac cagcgtgagc
     4681 tactaagccc atacgtagaa aagtattttg ctgcactaac tgaaatttgg aagtcgcgca
     4741 ctaatgaaat tgctcagtca ttaacgactg gtttattccc atccctgctt gcatctgaag
     4801 atattttgaa acgagcagat gcctttttgg caaccgataa agatgcaggc tccacgaggc
     4861 tgattaagga attacgtgac aatgttgcta gatcgcttcg ctgtcaggct aaagacagtt
     4921 aactaaagtt ttaatacttc gttaaatctt tagacttctt agtccaacct ggcgcaagca
     4981 caaaaagtgt gatcagcgct gtgattgcca gtggaattaa accgaaaagt acaattgcgg
     5041 ttgatgcatt taggactgag cctggctcat ctccatcatc acgcttgtta gcaatggccg
     5101 gggcaacaaa gctagcgctt aaaacgagtg ttgaagtcgt aattgcaaac gcacgtcgaa
     5161 gtctcatgta aatagcctac gatacgagtt ttgttaacac gacttcgcgc taattcacga
     5221 tttagtcttg ttttgctaga caattaatag gtaaacagac ggggggtttc atgccagatt
     5281 tatttgagtg gtttctgggc actccggtca aaatcgccgt aataatactg ttggcatttt
     5341 tacttcgagt ggttctgatc agagcttcaa gtagagttat taagcgagca cttctaatcc
     5401 gccgcgatcg ggccaagcaa tcaggtaatg cacaaaatga atctgccgcc caagatagat
     5461 atgaacaaag agcgcgttca ataagtgcac tgttacgcag cgtaatttct gtaactgttt
     5521 ggagtttggc tgcaattatg gctttatcga acctaggtat tgacgttgct ccgatacttg
     5581 cctctgctgg tgttgttgga gtggctttag gttttggcgc gcaaacttta attaaagatt
     5641 ttcttgctgg aattttcatg attttagaag atcagtatgg agtcggtgac ttaatcgatg
     5701 tgggtccagc gattggcact gtcgaggaaa tcggcctgcg cgtgacacgt ttacgagatg
     5761 aaagtggcat cgtttggtac atgcgaaacg gcgaagttct tcgaattgcc aatcactctc
     5821 agtaaacaaa tgatggaaac tacactttat gagcaaatgg gtggcacgcc cttttttgaa
     5881 aaattagtca ttggttttta cgaaggtata aaaagcgatc cggtattaaa gcccatgtat
     5941 ccagaagagg attttgcagg tgctactcga aggctaacga tgtttcttag ccaatactgg
     6001 ggtggaccta cagattattc gcaagaacgt ggtcatccaa gattaagaat gagacacaat
     6061 gtcttcccaa taaattctga tgcacgagat cgctggttaa cccacatgtc caattcgttg
     6121 gcaaaactgg aaatagaacc acatctcaaa gaccaactat ggacctacct ggttactgca
     6181 gcacatagtt tggtaaacct gccagataac taaaccccag cgcgaaaaat gaaccgcaat
     6241 aggccagaat tgaagtcatg ttaagagcaa aagattggtg gcgggaagcc gtcgtttatc
     6301 agatttaccc gcgttcatat gcagacgcga gtaacgacgg tatcggcgac atccctggaa
     6361 ttcgctctaa agtttcctat ctaaagtctc ttggcataga tgcagtttgg ttaagtccgc
     6421 actatccatc accaatgcta gatgcaggtt acgacgtatc tgattatcgc gatgtcgaac
     6481 caatctttgg caaactcgaa gatatcgaag gtctaataaa agatcttcat gctgccgggc
     6541 taaaaatgtt tatcgacatt gtgcccaacc acacatcatg ggaacatgct tggtttaaag
     6601 aagctctagc taaccctcca gagaatgatc cagcgattcc agcaaaagct aggtacgccg
     6661 aaggcccttg ggcaaaatat cacttacttc gcggtcaaaa cgatggaaag tctgaaccga
     6721 ataattggga aagtgttttt ggtggacctg cttggcacga agtaaaagat ctattaggta
     6781 aaccaacggg ttggtggtac ttacatattt tcgataagtc acaaccagat cttgattggg
     6841 acaatcaaga agttcgtgcc gactttagac aaacccttcg cttttggttt gaaaaaggta
     6901 tcgatggatt ccgaatcgac gtagctcatg gaattgcaaa agctgacggt tatccagact
     6961 gtccagaaat tctcgatggc gatccaacaa ataaaaaccc ttactgggat caggatgctc
     7021 ttcatgaagt ctggcgtgaa tggcgcaaag ttgctgatga gtttgaacca caacgggtat
     7081 ttgttgcaga ggcttgggtt ttcccagccg aacgaaatgc aagatatttg cgaccagatg
     7141 aattgcacac tgggtttaac ttcccgtttt tgaaatgccc atgggaggcc gaaaaattac
     7201 gcgaaacaat cgacatgtcg atgaccgcaa atgcgattgc acaagctcct actacttggg
     7261 ttttagaaaa tcacgatgtt tggcgtgctg ctacgaggta cgcaccagtg attggtagtg
//
LOCUS       DBNHVN010000087         2359 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_608683, whole genome shotgun sequence.
ACCESSION   DBNHVN010000087 DBNHVN010000000
VERSION     DBNHVN010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_608683"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="AC16Y1_01625"
     CDS             complement(<1..252)
                     /locus_tag="AC16Y1_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018502565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HIR5831195.1"
                     /translation="MAKERGKKLIAQNRKARHDYAIEDTYEAGIVLVGTEVKSLRAGR
                     ASLVDGYALVEDGEIWLRGVNIPMYDEGSWTNHVPTRARK"
     gene            complement(261..1487)
                     /locus_tag="AC16Y1_01630"
     CDS             complement(261..1487)
                     /locus_tag="AC16Y1_01630"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR5831196.1"
                     /translation="MRASSSRVLSTLASVVLVLTTLSGTARADDALDQRVAAAFDALS
                     KASAAAAQAAKDLKAAQSKLPAARKALEEANALEAKARSEYKTAQINADVAKAAYGAA
                     LARLASKQAEIVILQKKVDQFARTIYQQGQTTQLGIILNSTSPTDLTLRLEAIRAISQ
                     ANAQDLAELEEAKIQLAIEADDAEQKRIEADRLEALAADALAIADAAADKAAKAKAEV
                     DELIRQEAVALAAAEADRQRVKRQYDELRAEQLRVSGVSKKVSKGSGDPQATGPLSWP
                     LPGRSAGGGVGPRIHPVYRYQSCHTGVDVSAPTGTPIQAAGSGVVITARWSNAYGNML
                     MIDHGGGLVTMYAHLTAFAVGNGAGVGNGQVIGYVGSTGWSTGPHLHFEVHVNGVPHN
                     PMGWFGASRTPVSCWR"
     gene            complement(1616..>2359)
                     /gene="ftsX"
                     /locus_tag="AC16Y1_01635"
     CDS             complement(1616..>2359)
                     /gene="ftsX"
                     /locus_tag="AC16Y1_01635"
                     /inference="COORDINATES: protein motif:HMM:NF038346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HIR5831197.1"
                     /translation="KIEVSIFLCGNSSVAFTCTGTVTEPQRAAIQTTLEGLTPTVQGV
                     FYESSADAYERFKEQFEGSSILANVSPDALPESFRVKIDNPDNFDIVSNAVGQMQGVE
                     SVQDQRKLLERFFQILTGLQTFALAIAIAMLFVTILLVMNTIRVAAFARRRETAIMRL
                     VGASNSTIRLPFILEAVVAATIGATIASIGMVLAKAFLIDMRLAPNLTFIAFVTWKEV
                     LLILPVLYLVGVAIASIASAVALRRHLKV"
BASE COUNT          616 a          575 c          506 g          662 t
ORIGIN      
        1 ttttcttgcc ctagtcggca cgtggttggt ccatgagccc tcgtcataca ttggaatatt
       61 tacaccccgg agccagattt ctccgtcttc aaccaatgca taaccgtcca ccaagctggc
      121 gcgtccagct cgcagagatt taacttcggt acctactaaa acaattccag cttcataggt
      181 atcttcaatt gcataatcgt gtctggcttt gcgattctga gcaatgagct ttttacctcg
      241 ctcttttgcc atttaaacca ctatctccaa caagaaacag gtgtgcggct tgcgccaaac
      301 caacccatcg gattatgtgg cacaccattt acgtgcacct caaagtgtaa atgaggtccg
      361 gtcgaccaac ctgtcgatcc aacgtagcca ataacttggc cgttaccaac tccagctcca
      421 ttaccaactg caaatgcagt taagtgagca tacattgtta ctaagccgcc accgtggtca
      481 atcatcaaca tgttgccata ggcgttactc caacgtgctg tgatcacaac acctgaacct
      541 gctgcttgaa ttggagttcc agtaggtgca cttacgtcaa cgccggtgtg acaactttga
      601 tatctgtaaa ccgggtgaat gcgcgggcct acgccaccac ctgcagatct tccaggcaat
      661 ggccacgaca gtggaccagt tgcttgtggg tcaccgctac ctttcgaaac ttttttagat
      721 actccagaaa cacgtaactg ttctgcacgt aattcgtcat actgacgttt aacgcgttga
      781 cgatcagctt cagcagccgc taacgctaca gcttcttgtc taattaattc gtcgacttct
      841 gcttttgctt ttgctgcctt gtccgcagca gcatctgcaa tcgcgagtgc gtctgcagca
      901 agtgcttcaa gtctgtctgc ttcgattcgt ttttgctctg catcatcagc ctcaatggcg
      961 agttgaattt ttgcttcttc aagttcagcc aagtcttgtg cgttagcctg cgaaattgct
     1021 cgaattgcct caagtcgcaa agtcagatct gtaggtgaag ttgagtttaa aataatgcca
     1081 agttgagtgg tttggccttg ttgataaata gttcttgcga actgatcaac tttcttttgc
     1141 aaaataacta tttcagcttg tttacttgcc agtctggcta gcgctgcgcc gtaagcagct
     1201 ttagccacat cagcattaat ttgtgcagtt ttatattcac ttcttgcttt tgcttctagt
     1261 gcattcgctt cttccagagc cttacgagca gccggaagtt ttgactgagc agcttttaag
     1321 tccttggctg cttgcgctgc tgctgcagat gctttagaaa gtgcgtcgaa agctgcggca
     1381 actcgctgat caagggcatc atcggcgcgc gcggtgccgg aaagagtggt taaaactaaa
     1441 acaacggagg ccaatgtact taaaacacgc gaggaagatg cgcgcatgag ccaggctccg
     1501 ttctatgggg aagttctttt agattaactg aagttactgg tgttactcat gttaaaaagc
     1561 cgtgtgttga gggttttttc ctgaatttca ggcattttta accgttttaa gtggcttaga
     1621 ccttcaaatg tctgcgcaat gcaacggccg aggcgatcga agcgatcgcg accccaacta
     1681 gatacaaaac tggcaaaatc agcaaaactt ctttccaggt aacaaatgcg ataaaagtta
     1741 aattaggcgc caatcgcatg tcaataagaa acgccttggc caaaaccatt ccaatagaag
     1801 caattgttgc tccgatggtt gccgctacaa cagcttccaa aatgaaaggc aatcgaatcg
     1861 ttgaattgct cgcaccaact agccgcataa ttgccgtttc acgacgacgt gcaaaagcag
     1921 ccactcgaat ggtgttcatc acgagcaaaa ttgtcacaaa caacattgcg attgcaattg
     1981 ctagtgcaaa ggtctgcagg ccggtcaaaa tctggaagaa acgttctaaa agttttcgtt
     2041 gatcttgaac agattcaaca ccctgcatct gcccaaccgc gtttgaaaca atatcaaaat
     2101 tatctgggtt atcaattttt actcggaaag attctggtag tgcgtctgga cttacgtttg
     2161 ccagaatcga tgaaccttca aattgttctt taaatctttc ataggcatca gcagaagact
     2221 cataaaatac accttgaacg gttggggtta aaccttcaag tgttgtctga attgctgccc
     2281 gctgtggttc agtcactgtt ccagtacaag taaatgcaac cgacgagtta ccacacaaga
     2341 aaattgatac ttcaatttt
//
LOCUS       DBNHVN010000088         1357 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1130085, whole genome shotgun sequence.
ACCESSION   DBNHVN010000088 DBNHVN010000000
VERSION     DBNHVN010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1130085"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="AC16Y1_01640"
     CDS             <1..1095
                     /locus_tag="AC16Y1_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831198.1"
                     /translation="VVRNTPILSGPVNTGTNLNQISNRPQTLIGGVPTNTTTRIVDQS
                     KLNVLAGALNLEIQVPQNVGSVTNNGGSTQLQVQKGGVTTINGSGLRPLSTVQVFLPL
                     GGNNSRELARIPVTSSGTFNGLATFANPTQGAPLPIGPQVLQIVSVDSAGRQAVVEMK
                     VSIAQPAPAPEQNRANGQLPELTPGQSIATEAGLPVPVTVEAIEQQRQAIIQGDTWNM
                     SINVPNANGEVTKTEGGALLKFVQNESVFVEGDGFLPGTRADVWLFSDPTLLGTVEID
                     ENGKFAGQIAVDGKFVPTGEHTLQVQGVGDDGFVRSANLGVIVEANPVSATSGITSIW
                     IYALVAVGLGATWWFFLIARRRRKEAKHTS"
     gene            1125..>1357
                     /locus_tag="AC16Y1_01645"
     CDS             1125..>1357
                     /locus_tag="AC16Y1_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation efflux transmembrane domain-containing
                     protein"
                     /protein_id="HIR5831199.1"
                     /translation="MSSKLLNYFDLRRTVLLVAALNFLYFGVEFTFGRLYNSLALISD
                     SIDFLEDASVNLLVVLALGWSVAKRKYVSYLLAA"
BASE COUNT          370 a          292 c          305 g          390 t
ORIGIN      
        1 gttgtgagaa atactccgat tctttctgga cctgtaaata ctggtactaa tctgaatcag
       61 atttcaaatc gacctcaaac tttaattggt ggcgttccaa ccaatacaac cactcgaatt
      121 gttgatcaaa gcaagttaaa tgttttagcc ggtgcgttga atcttgaaat tcaagttcca
      181 caaaacgttg gttctgtcac aaataatggc ggctcaacac agcttcaggt tcaaaaaggt
      241 ggagtgacca caattaacgg aagtggccta cgcccacttt caacagttca agtattcctt
      301 cctttagggg gaaataattc aagagaactt gctcgaattc cagtaacttc atctggaaca
      361 tttaatggat tagctacttt cgctaatcca actcaaggtg cgccactacc aattggccca
      421 caagtgctgc aaattgtcag tgttgattct gctggtcgcc aagcagtagt tgaaatgaaa
      481 gtgagcattg cccaacccgc tccggctcca gaacaaaacc gtgctaacgg tcagttaccc
      541 gaactaactc ctgggcaatc aattgcgaca gaagctggct tgccagttcc ggtaacggtg
      601 gaagcaattg aacaacaacg ccaagcgatc atccagggtg acacttggaa catgtcgatt
      661 aacgtgccaa atgcaaatgg cgaagttaca aaaactgaag gcggagcatt acttaagttt
      721 gtccagaatg aatcagtatt cgttgaaggt gatgggttct tacctggaac tcgcgcagat
      781 gtttggctgt tctctgaccc aactttgctt ggcacagttg aaattgatga gaacggtaag
      841 tttgcaggac aaattgcagt cgatggcaag tttgttccaa ctggcgaaca cacacttcaa
      901 gttcaaggtg ttggtgatga cggctttgtt cgctcagcaa acctaggtgt gattgttgag
      961 gccaacccag tttctgcaac ttctggaatt acatccatct ggatttatgc tttggtggca
     1021 gttggtctgg gtgcaacctg gtggttcttc ctgatcgccc gtcgcagaag aaaagaagct
     1081 aagcacacgt cataacctga cttgtacggc atagtattta aaacatgtct agcaagttgc
     1141 ttaattactt tgacttgcgt cggacagttc tattagttgc cgcgcttaac tttctttact
     1201 ttggtgttga attcacattc gggcgacttt ataactcgct tgcattaatt agtgacagca
     1261 ttgattttct agaagatgca tctgtaaatc tgttagttgt tctagctctc ggttggtcag
     1321 ttgccaaacg taaatacgtt agctatttat tggctgc
//
LOCUS       DBNHVN010000089         2543 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1501553, whole genome shotgun sequence.
ACCESSION   DBNHVN010000089 DBNHVN010000000
VERSION     DBNHVN010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1501553"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1513)
                     /gene="uvrC"
                     /locus_tag="AC16Y1_01650"
     CDS             complement(<1..1513)
                     /gene="uvrC"
                     /locus_tag="AC16Y1_01650"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR5831200.1"
                     /translation="MQAVDKPRDVPTNPGVYRFRDETDKVIYVGKAKNLKNRLNSYFS
                     GELQPRTKTMVETATAVDWVVVRTEIEALQLEYAWIKEYDPTFNVRFRDDKSYPYLAV
                     TVNEDFPRAFVYRGQRKKGTKYFGPYVQIGSINETLDHLLRVFPVRSCTAGVYKNAKR
                     ANRPCLLAHIERCSAPCVGDISQDEHRKLVEKFLSFMNGNTKAIIKEITDQMQAASSA
                     QDYEKASVLRDDLAALERVQQKSTVVFSEETDADLVAVAHDDLQAAVQIFNVRNGRIR
                     GQRGFIMENSVESTTGSLVTDALMHIYANADKADLPPEILTNEMPDSPESLHQLLMEV
                     RKANLDIRVPQRGDKKTLLETVFTNAGQALVSHKSKRSTDLIARTQALNELQEALGLA
                     RAPLRIECIDVAHIQSTNVVASLVVFEDGLPLKKDYRHYLVEGLPDDTRRIAQVVERR
                     FAAHKEQSGPYRPNLLVIDGGLPQVNAAARALTDAGVFNMHVIGLAKRLEEIWL"
     gene            complement(1500..>2543)
                     /locus_tag="AC16Y1_01655"
     CDS             complement(1500..>2543)
                     /locus_tag="AC16Y1_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008357183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990391 - DNA repair complex [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrA family protein"
                     /protein_id="HIR5831201.1"
                     /translation="GPKKRRPQDPKRKLTVQGAAEHNLKNVSVDFPLGNFVAVTGVSG
                     SGKSTLVNDILFNVLARDLNRARTVPGRHKKVLGVDLLDKVVHVDQSPIGRTPRSNPA
                     TYTGVFDHVRKLFAETPEAKMRGYLQGRFSFNVKGGRCESCSGDGTLKIEMNFLPDVY
                     VPCEVCHGARYNRETLEVHYKGKTIADILNMPIEEALEFFAPIQTIARHLQTLVEVGL
                     GYVRLGQPATTLSGGEAQRVKLSTELQKRSTGKTVYVLDEPTTGLHFEDVRKLLAVLH
                     SLVEKGNTVIVIEHNLDVIKTSDWIIDMGPEGGSGGGTVVATGTPEAVARITASHTGK
                     FLAPMLKTKNASR"
BASE COUNT          661 a          598 c          545 g          739 t
ORIGIN      
        1 gcagccaaat ttcttcgagt cttttagcta aaccaattac atgcatgttg aaaacaccag
       61 catcagttaa cgctctggca gcggcgttca cctgaggtag gccaccgtcg atcactagaa
      121 ggtttgggcg atatggaccg gattgttctt tatgcgcagc aaacctacgc tcaacaactt
      181 gagcaatccg acgagtatcg tcgggtaaac cttcaactag gtaatggcgg taatcctttt
      241 taagtggcaa cccatcttcg aataccacca gtgaggcaac cacatttgta ctttgaatgt
      301 gggctacgtc aatacattca attcgaagtg gcgccctagc taagccgagt gcttcttgaa
      361 gttcgttaag tgcctgcgtt ctagcaatta agtcagttga gcgctttgat ttatgagata
      421 ccaaagcttg accagcgttg gtaaaaacag tttcaagtaa agtttttttg tcgccccgct
      481 gtggaacccg aatatctaaa tttgcttttc gtacttccat tagtagttga tgcaaacttt
      541 caggactatc tggcatttca ttcgtgagta tctcaggtgg caaatctgcc ttgtctgcat
      601 tcgcatagat gtgcattaac gcatctgtta ccagcgagcc agtggtagat tcaacggaat
      661 tttccataat gaaacctcgt tgcccgcgga ttctgccgtt tcgtacatta aagatttgca
      721 ctgccgcttg cagatcgtca tgagcaactg cgacaagatc agcatcagtt tcttcactaa
      781 ataccacagt gcttttttgt tgcactcttt ctagagcggc tagatcatca cggagcactg
      841 atgctttctc gtaatcttga gcgctggatg ctgcttgcat ttggtcggtg atctctttga
      901 tgatggcctt tgtgtttccg ttcataaaag ataaaaattt ttcaacaagt ttgcgatgtt
      961 cgtcttggga aatatctcct acacatggtg cactacaacg ttcaatatgg gccagtaaac
     1021 atggtcgatt agcacgtttc gcattcttgt agacaccagc cgtacaactg cgtactggaa
     1081 agactcgaag taagtgatct aacgtttcgt tgatactgcc aatctgaaca taaggaccaa
     1141 aatacttagt cccctttttg cgttggccgc gataaacaaa agcgcgtgga aagtcctcat
     1201 ttacggtcac agctaagtaa gggtaagact tatcgtcacg aaatcgaacg ttgaatgttg
     1261 gatcatattc tttaatccac gcatactcga gttgcagggc ctcgatttca gttcgaacaa
     1321 ctacccagtc aactgcggtt gccgtttcaa ccattgtttt tgtccgaggt tgtaactccc
     1381 ctgaaaaata tgaatttaag cggttcttta aatttttagc tttgccaacg taaattactt
     1441 tgtcggtttc gtcgcgaaac cgatacacgc cagggttagt tggcacatca cgtggtttat
     1501 caacggcttg catttttagt ttttaacatt ggggccaaga attttccagt gtgactagca
     1561 gttatcctgg caactgcttc gggtgtccca gttgccacca cggtaccgcc gccactgccg
     1621 ccttcaggac ccatatcgat gatccaatct gacgttttaa tcacatctaa attatgttcg
     1681 ataacgatta cggtattacc tttttcaact aatgaatgta aaactgcgag taatttgcgc
     1741 acatcttcga aatgtaagcc ggttgtcggt tcatcaagaa cgtaaactgt tttacccgtc
     1801 gagcgctttt gtagttcagt tgaaagcttt acccgctggg cctcgccacc gcttaacgta
     1861 gtcgcaggtt gaccaaggcg aacgtaaccc aaaccgacct caactaatgt ttgcaaatgt
     1921 cgagcgattg tttgaatcgg cgcgaagaat tccaaagcct cttcaattgg catattcaag
     1981 atgtcggcga tggtcttgcc tttgtagtgc acttcgaggg tctcgcggtt ataacgcgcc
     2041 ccgtgacaaa cttcacatgg cacgtataca tctggcagga agttcatctc aatttttagc
     2101 gtgccatcac cagaacaaga ttcacagcga ccgcctttta cgttaaacga gaagcgacct
     2161 tgtaaatacc cgcgcatttt tgcctctggg gtttcggcaa ataacttacg cacatgatca
     2221 aataccccgg tataagttgc tggattagac cttggcgtgc gtccgattgg actttgatca
     2281 acatggacaa ctttgtcgag taaatcgaca cccagaactt tcttgtgtcg acctggaaca
     2341 gttcgagccc gattcaaatc gcgagccagc acattaaaca aaatgtcatt tactagcgtt
     2401 gatttaccag atccagaaac accagtaact gcaacaaaat taccaagtgg gaaatcaact
     2461 gaaacatttt ttaaattgtg ctcagctgcc ccttgcacag ttaacttacg tttcgggtcc
     2521 tgcggtcgac gttttttagg acc
//
LOCUS       DBNHVN010000090         4156 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1278973, whole genome shotgun sequence.
ACCESSION   DBNHVN010000090 DBNHVN010000000
VERSION     DBNHVN010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1278973"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..594
                     /locus_tag="AC16Y1_01660"
     CDS             142..594
                     /locus_tag="AC16Y1_01660"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5831202.1"
                     /translation="MQNDMVQIRKAVETDLVQLTQLVEQYRDFYQQQPNSQTSSFLLD
                     RLKNNESVIFVAQVDENLVGFTQCYPSFSTVSVSSVWLLNDLFVEPEHRGKDIANKLM
                     RAAEEAAKSAGASRIWLRTSHTNEPAQKLYESRGWKQDEVFRRYDLIF"
     gene            complement(612..857)
                     /locus_tag="AC16Y1_01665"
     CDS             complement(612..857)
                     /locus_tag="AC16Y1_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HIR5831203.1"
                     /translation="MITMYGAEWCGDCRRSTALLNELGVEFNYINVEEVPEAADKVVE
                     INGGAQSIPVIVFEDGTHLTEPSNPDLRNKLESLGII"
     gene            complement(922..2367)
                     /gene="glnA"
                     /locus_tag="AC16Y1_01670"
     CDS             complement(922..2367)
                     /gene="glnA"
                     /locus_tag="AC16Y1_01670"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR5831204.1"
                     /translation="MSNLGLKNAQDVLKYIKDNNIKFVDVRFCDLPGVQQHFNMPVES
                     VNEALFTDGQMFDGSSIRGFQSIHESDMKLIPDPTTAFIDPFREAKTLVMNFSVHDPF
                     TDEPYSRDPRNVAAKAQAYLASTGIADTAFFAPEAEFFIFDSVRFSTATNESFYHIDS
                     VEGAWNTGSEFNADGSDNRGYKTPTKGGYFPVSPTDQMADLRDSIVTKLGEVGLLVER
                     SHHEVGSPGQMEINYRFNTLLKAADEVMLFKYVVKNQAWAQGKTATFMPKPIFGDNGS
                     GMHVHQSLWSGGKPLFFDERGYGGLSDMARWYIGGILKHAPSLLAFTNPTVNSFHRLV
                     PGYEAPVNLVYSQRNRSACIRIPITGSNPKAKRIEFRCPDPSSNPYLAFAALLMAGLD
                     GIKNKIEPHAPVDKDLYELPPDEAADIPQVPSSLPEVLDALEADHDYLMEGGVFTQDL
                     IDTWISYKRTAEVDYVRLRPHPAEFELYYNL"
     gene            2533..3006
                     /locus_tag="AC16Y1_01675"
     CDS             2533..3006
                     /locus_tag="AC16Y1_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR5831205.1"
                     /translation="MDRKDIGSWLLGPRAALEQQGISFGYPGERLGIPEHGVGSVATF
                     SRRILAITIDWLSALLIVRGFFQTIEYGSTDFSVAALIVFASQVFLLTTTLGASFGQQ
                     VVGIGVRKVTGQKLGLPAALIRSVLLTLVLPAVIWDRDGRGLHDKTVKSVVVKTR"
     gene            complement(3021..3242)
                     /locus_tag="AC16Y1_01680"
     CDS             complement(3021..3242)
                     /locus_tag="AC16Y1_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831206.1"
                     /translation="MQQITDFLSSLTPTQWIIAIVVAVMLAKKLVKLAIVIGLIVFVA
                     FPYIKSSGLLDFNFDPNSILQQVLNQLGL"
     gene            complement(3242..3904)
                     /locus_tag="AC16Y1_01685"
     CDS             complement(3242..3904)
                     /locus_tag="AC16Y1_01685"
                     /inference="COORDINATES: protein motif:HMM:NF025199.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4191 domain-containing protein"
                     /protein_id="HIR5831207.1"
                     /translation="MSTTNSAPKKKRWYRQVQETYSFAKSAIPGLPFKLLGIFLGVFA
                     ALLAIGFLIGQPIFLGLTGFSIALLVTLIVFGRWAERAAYQSIEGQLGAAGSVLSAMR
                     GAWFISAGIGVDKNQNIAHRVVGRPGIILVGEGSRPGSLLAEQRKAHMRYVPNVPIHE
                     LIVGEGKLTISNLQKTIKKLPKALRPAEVTDVRRRLDALPKVALPIPKGPMPQGRKIP
                     RR"
     gene            complement(3915..>4156)
                     /locus_tag="AC16Y1_01690"
     CDS             complement(3915..>4156)
                     /locus_tag="AC16Y1_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR5831208.1"
                     /translation="LNDLHNAGCDLVTITQYLRPSLRHHPVTRWVKPEEFVELANFAT
                     EIGFAGVMSGPLVRSSYRAGRLYQQALAARKISTV"
BASE COUNT         1129 a          904 c          958 g         1165 t
ORIGIN      
        1 ggtctattaa ttggtggagt tgagcttttg gttcgatatg tcgaggggct gggtggcgca
       61 ttaggcagca tgctacttgg cattgtttta ctggtgattg gcaccagact gtttagagat
      121 aagcagtaaa gatacgggat agtgcaaaac gatatggtgc aaattcgaaa agcagtggag
      181 actgacttag ttcaattaac tcaattagtt gaacagtacc gagattttta tcagcaacag
      241 ccaaactcac aaactagttc gtttcttcta gatcgcctta agaataatga gtcagttatt
      301 tttgttgcgc aagtagacga aaatttagtt ggcttcactc agtgttaccc gtcattttcg
      361 accgtctcag tttcatctgt ttggttgctc aatgatctct ttgtggaacc cgaacatcgc
      421 ggtaaggaca ttgcaaataa gttgatgcgg gctgcagaag aggcagctaa gtctgccggg
      481 gcatcaagaa tttggctgcg cacctctcac accaatgaac cagcacaaaa actttacgag
      541 agtcggggct ggaaacagga cgaagtcttc cgacggtatg acttaatctt ttagctttgt
      601 gcaaaagcta gttaaataat tcctagcgat tcaagtttgt tacgtaaatc tgggttactt
      661 ggctcagtta agtgagtacc gtcttcaaat acgatgactg gaattgactg ggccccacca
      721 ttgatttcaa cgactttatc tgctgcttct ggaacttctt cgacgttgat gtaattaaat
      781 tccacaccaa gttcatttaa aagtgctgtt gaacgtcggc aatcgccgca ccattctgcg
      841 ccatacatag taatcatgtg tttaagtcta acttgaaata aaaagccccg cggaaaatac
      901 cgcggggctt ttaagtagtc cttataggtt gtagtacaac tcaaattcgg caggatgtgg
      961 ccgtaaccga acgtaatcaa cttcagcagt tcgcttgtac gaaatccaag tgtcgattag
     1021 atcctgggtg aatacaccac cttccatcag gtagtcatga tcggcttcaa gtgcatcaag
     1081 cacttccgga agcgaacttg gtacttgtgg aatgtcagcg gcttcatctg gtggtaactc
     1141 gtaaaggtct ttgtccactg gcgcatgtgg ttcgattttg tttttaattc catctaagcc
     1201 agccattagt aacgcagcaa aagctaggta cggattactt gatgggtcag gacaacggaa
     1261 ctcaatgcgc tttgcctttg ggttacttcc agtgattgga atgcgaatac atgccgatcg
     1321 attgcgctgt gagtaaacca aattaactgg tgcctcataa ccaggcacta accgatggaa
     1381 tgagttaact gttgggtttg taaaagcaag tagcgacggt gcgtgcttca agatgccacc
     1441 gatgtaccac ctagccatgt cggataagcc accataacca cgttcatcaa agaacagtgg
     1501 tttgccacca ctccataatg attggtggac gtgcatacct gacccattgt caccaaagat
     1561 tggttttggc atgaacgtcg cagtcttgcc ttgtgcccaa gcttggtttt taacaacgta
     1621 cttgaatagc atcacttcat cggctgcctt tagcagtgtg ttaaagcggt agttaatttc
     1681 catctggcct gggctaccaa cttcgtgatg tgaacgttct actaataagc caacttcacc
     1741 aagtttggta acgatcgaat cgcgcagatc tgccatttga tcagttggtg aaacagggaa
     1801 gtagcctccc tttgtcggag ttttgtatcc acgattgtct gaaccatcag cattaaattc
     1861 actcccagta ttccaagcgc cttcgactga atcgatgtga taaaacgatt catttgtagc
     1921 agtactgaag cgcacagaat caaaaataaa gaattcagcc tcaggcgcaa agaatgcagt
     1981 gtcggcaatt ccagtcgatg ctaagtacgc ttgagccttc gcagcaacat ttcgcggatc
     2041 acgactatag ggttcatccg taaatgggtc gtgtactgag aaattcatca ccaaagtttt
     2101 tgcttcgcgg aatggatcga taaaagcagt tgtcggatct ggaattaact tcatgtcaga
     2161 ttcatgaatc gactggaagc cgcgaattga ggacccatca aacatttgac catcagtgaa
     2221 aagggcctca tttactgact caaccggcat gttaaagtgc tgttgcaccc caggcaggtc
     2281 gcagaatcta acgtcaacaa atttaatgtt gttgtctttg atgtatttaa ggacatcttg
     2341 ggcgttttta agacctaagt tggacatcca tcctccagct aatagtgaat tcggttaatt
     2401 tcatatttgg gccgcgaacg actaatccag acgatagcgc tggttagtta aaaagccgtg
     2461 tctcgggtgt ttcagatttg ttacgggccg tcccagatgc cacttttatt tatttcatta
     2521 ggctaaatac ctatggatcg caaagacatt ggctcgtggc tacttggccc gcgagcggcc
     2581 ctagaacagc aggggattag ttttggctat ccgggcgagc gcttgggaat cccagaacat
     2641 ggtgtcggct cagtggcgac gtttagccgg cgaattttag cgatcacaat tgattggcta
     2701 agcgcactat taatcgtccg tggttttttt caaacaattg aatacggcag tactgatttt
     2761 tcggttgcag ccttaatcgt ctttgcaagt caagttttct tactgaccac cacacttgga
     2821 gcaagttttg gtcagcaagt agttggtatc ggagtccgca aagtcaccgg tcaaaaactt
     2881 ggcttgccag ctgccttgat tagaagtgtt ttactaactc tcgttttgcc tgcagttatt
     2941 tgggatagag acggtcgcgg actccacgat aaaactgtga agtcagtcgt ggtaaaaact
     3001 cgctaacgct aagacttgtt ttataagccc aactggttta gcacttgttg caaaatactg
     3061 tttggatcaa agttgaaatc aagtaaccct gaacttttaa tgtacgggaa cgcaacaaaa
     3121 acaataagac caattacgat ggcgagtttt actaactttt tagccagcat cactgcgacc
     3181 acaatggcga taatccattg agtaggagtc agtgaactta gaaaatctgt gatctgttgc
     3241 attatcgtct cggaatcttt ctaccttgtg gcattggacc tttaggaatt ggtagagcta
     3301 cttttggtag tgcgtctaat ctgcgtcgaa cgtcagttac ctcagccgga cgtaacgcct
     3361 taggtaactt tttaattgtt ttttgtagat tggaaattgt taactttcct tcgccaacaa
     3421 ttaattcgtg gattggcaca ttgggcacat aacgcatatg tgctttacgt tgctcggcaa
     3481 gtaatgaacc ggggcgacta ccttcaccaa ccaaaataat tccaggtcta ccaactacgc
     3541 gatgggcgat attttggttt ttgtcgacgc cgattccagc acttataaac caagctccgc
     3601 gcattgcact aagtactgat ccagcagcac caagttggcc ttcaatcgat tgataagcgg
     3661 cacgttctgc ccatcgacca aaaacgatca atgtaacaag tagtgcaatt gaaaaaccag
     3721 ttagtcccaa gaaaataggt tggccaatca gaaagccgat tgctagtaaa gcagcgaata
     3781 ccccgagaaa aatacccagt agcttaaatg gcaagccagg aattgctgac tttgcaaatg
     3841 aataagtctc ttggacctgg cggtaccaac gcttcttttt cggtgcacta tttgttgttg
     3901 acatgccttt taccttagac ggtcgatatc tttctagcag ctaaggcttg ctgataaagg
     3961 cggccagccc ggtatgaaga cctaaccaac ggaccgctca taacaccggc aaagcctatt
     4021 tcagttgcaa agttagccaa ctcgacgaat tcttcgggtt taacccagcg ggttaccggg
     4081 tgatggcgaa gtgaaggccg taaatattgg gtaatggtga ctaagtcgca gccagcatta
     4141 tgcagatcat taagcg
//
LOCUS       DBNHVN010000091         3085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_461759, whole genome shotgun sequence.
ACCESSION   DBNHVN010000091 DBNHVN010000000
VERSION     DBNHVN010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_461759"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /locus_tag="AC16Y1_01695"
     CDS             <1..1507
                     /locus_tag="AC16Y1_01695"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR5831209.1"
                     /translation="TVLARNVTAYQTLIKDPAGAAALLAPVLGLPIEQIIPALTGDKK
                     FSYVAKRITPEKWKEVAALDIAGIFSEPTTSRIYPNGTLAASIVGYVGAEGRGLGGLE
                     FGIDEILAGKDGQVQVERVNGREIPGSERQSVDPVEGLSVNLTIDRDIQAVAERALVE
                     QVQKAGASGGSVVVMKKNGEILAIANAPVFDPNNPFATDDRSKRNMAVTDVFEPGSTS
                     KVMTIAAVINEGVASPETPFTIGSSIPRGGTSFKDHDEHGVIQLTLNGVLAKSSNIGT
                     IMASELIGQDKFYEYLKRFGIGDLTGLNFPGESGGLLPDPSNPNQWSGTTFPTLAFGQ
                     GLSVNAVQAASVYATIANDGMRVAPRLISSFSRSDGSIEYAKEPKSVQIVTPQTAKIM
                     WEMLMSVVSDQGTAPQAQIPGYQIAGKTCTANKYSEVTGGYSGYTASFIGIAPGNNPE
                     LIVAVMIHDPVNGHYGSTISGPVFKQVMTYALAHQKIPPTDTPQPQIPVTW"
     gene            1514..3013
                     /locus_tag="AC16Y1_01700"
     CDS             1514..3013
                     /locus_tag="AC16Y1_01700"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR5831210.1"
                     /translation="MRPKTSPKQLNQIATGFNLTSESSNGSVLISGITHNSKEVLSDD
                     IFVALPGANHHGQQFMSQALTNGAVAIATDETGFASAISTGLPVIKLESPRKDMATLA
                     ARVYDHPEEKLTLVGVTGTNGKTSVTHILQSIFSQFGFKVGVIGTVGTYLAGQHLSGV
                     RTTPESTDLYAILALMAEKEITHVFMEVSSHALAQDRVHGLKFTKAIFTNLSQDHLDF
                     HGTMENYFAAKAKLFTPDFAKSAIICAEDNWGEKLALQTKISNTTVGIQSHQNNQSNQ
                     NTDWLISDLRLTVTQTDFILRHEHQDFACSLPMIGAFNATNAALAVVAASELGLDPKL
                     VTKQLVQITAVPGRSQLITHNSPGTAIVDYAHTPEAVEKVLTTLREVCAGQLITVIGC
                     GGDRDATKRPIMGKVAAEISDVIIVTDDNPRSENPAKIRAAVLAGITDQNIEVLEIGD
                     RREAIKQALAKASHADIVAVLGKGHEQGQEVAGQVFPFDDVQVIQQEAN"
BASE COUNT          924 a          661 c          708 g          792 t
ORIGIN      
        1 cactgttcta gccagaaatg taacagctta tcaaacttta attaaagatc ctgccggagc
       61 tgctgcatta ctagcaccgg tactgggttt accaatagag caaataattc cagcactgac
      121 tggagacaag aaatttagtt acgtcgcaaa gcgcatcacc ccagagaaat ggaaagaagt
      181 tgcggcactt gatatcgccg gtatttttag tgagccaacg acaagccgaa tttatccaaa
      241 tggaacatta gccgccagca ttgttggtta tgttggcgca gaaggtcgcg gtttaggtgg
      301 cttggaattt ggtattgacg aaatacttgc cggtaaagac ggtcaagttc aagttgaacg
      361 agttaacggt cgagaaattc caggcagtga gcgacaaagt gttgatccag ttgagggact
      421 ttcagttaac ttaacaattg atcgcgatat tcaagcagtt gctgaacgcg cacttgttga
      481 gcaagtgcaa aaagctggtg catctggtgg ctcggttgta gtgatgaaaa agaatggtga
      541 aatattggcg attgccaatg caccggtttt tgatccaaat aatccgtttg caacggatga
      601 tagatcaaag cgaaatatgg cagttacaga tgtttttgag ccaggttcaa ccagtaaagt
      661 catgacaatt gccgcagtaa tcaacgaagg cgtagctagt cctgaaaccc cattcactat
      721 tggttcaagc attcctcgcg gtggcacctc ttttaaagat cacgatgaac atggtgtcat
      781 tcagctaaca cttaatgggg tacttgcaaa gtcaagtaac atcggcacaa tcatggcttc
      841 agagctaatt ggccaagata agttttatga atacttaaag cgctttggaa tcggtgacct
      901 gaccggactc aatttcccag gtgaatctgg tggtctactt ccagatccaa gcaatcccaa
      961 ccaatggtct ggcacaactt tcccaacatt ggcatttggc caaggcttat cggttaatgc
     1021 agtgcaggct gcatctgttt acgcgactat cgcaaatgat ggcatgcgcg tagctccaag
     1081 attaatttcg agtttctcta gaagtgacgg cagtatcgag tacgccaaag agccaaaaag
     1141 tgtgcagatt gtcacaccac aaacagcaaa aatcatgtgg gaaatgttga tgagtgttgt
     1201 ttcggatcaa ggtaccgcgc cgcaagcgca aatacctgga tatcaaatag ccggtaaaac
     1261 gtgtaccgca aataagtaca gcgaagttac tggtggatat agcggataca ccgcatcttt
     1321 catcggtatt gcacctggaa ataatccaga actgattgtg gcagttatga tccatgatcc
     1381 ggttaatgga cattacgggt cgacaatttc cggtccagtt ttcaagcaag ttatgactta
     1441 cgcgctggca catcaaaaga ttccgccgac agatacgccg caaccgcaaa tcccagtaac
     1501 gtggtaatta gtcatgcgac caaaaacatc acccaagcaa ctcaatcaaa ttgcaactgg
     1561 gtttaatctc accagcgagt catcaaatgg ctcggtttta atatctggaa taacacataa
     1621 ctcaaaagaa gttttatctg atgatatttt tgttgcacta cccggtgcaa atcatcatgg
     1681 tcaacaattt atgtcgcagg cgttaacgaa cggtgccgtt gcaatcgcaa ctgatgaaac
     1741 tggttttgct tcagcgattt caactggctt gccagtaatt aaacttgagt cgccacgtaa
     1801 agatatggca actttggcag ctcgagttta tgatcacccc gaagaaaaat taactttagt
     1861 tggggttact ggcacaaacg gcaaaacttc ggtaactcac attctgcaat ctatatttag
     1921 tcagtttggt tttaaagttg gggttatcgg tacagtcgga acctatcttg ctggccaaca
     1981 cttaagtgga gttcgaacta ccccagagtc cactgatctt tacgcaatcc ttgcgttgat
     2041 ggccgaaaaa gaaataacac atgtattcat ggaagtttca agtcacgcgc ttgcccaaga
     2101 ccgggtgcac ggactgaaat tcactaaagc gattttcacg aacctaagtc aggatcattt
     2161 agattttcat ggcacgatgg aaaactattt tgccgcgaaa gcaaagttat ttacccctga
     2221 ttttgctaaa agtgcgatta tttgtgccga agataattgg ggcgaaaaat tagctttaca
     2281 aacgaaaatc tccaacacca cagttggcat ccaatcccac caaaataatc aatccaacca
     2341 aaataccgac tggttaattt cagatttacg cctaacggtt acgcaaacag acttcatttt
     2401 gcgacacgag caccaggact ttgcctgctc attgccaatg attggggctt tcaatgcaac
     2461 taatgcggca ctggcagttg ttgcagcaag cgaacttgga ttagatccta aattagttac
     2521 gaaacaactc gttcaaatta cagccgttcc tggtcgtagc cagttaatta cgcataacag
     2581 cccaggcact gcaatagttg attacgccca cactccagag gcagtagaaa aagttttaac
     2641 aacattgcga gaagtgtgcg ctgggcagtt aatcactgtc ataggttgtg gcggtgatcg
     2701 tgatgccact aagcggccaa tcatgggcaa agttgcagcc gaaatttcag atgtaattat
     2761 tgtcaccgac gacaatccgc gatctgaaaa tccagccaaa attcgcgcag ctgtgttagc
     2821 tggcatcacg gatcaaaata ttgaagtgct cgaaatcggc gatcgtcgcg aggcaatcaa
     2881 acaggcgcta gctaaagcaa gtcacgctga catagtcgca gtgctaggta agggacatga
     2941 gcaagggcaa gaagttgccg gtcaagtttt tccatttgat gacgtgcaag ttattcagca
     3001 ggaagctaac tgatgtttag attaacccta aatgaaattg cacatgtagt cggtggaaaa
     3061 gttgttggtg atggggaatt agtcg
//
LOCUS       DBNHVN010000092         2389 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1576426, whole genome shotgun sequence.
ACCESSION   DBNHVN010000092 DBNHVN010000000
VERSION     DBNHVN010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1576426"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..371)
                     /locus_tag="AC16Y1_01705"
     CDS             complement(162..371)
                     /locus_tag="AC16Y1_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831211.1"
                     /translation="MLIDCNSCVMKDIACGECVVTMLLGPVPESIDDHKDALTVLAQA
                     GIVAPLRLIKGDGENPHAPAAASNL"
     gene            complement(449..1525)
                     /gene="trpD"
                     /locus_tag="AC16Y1_01710"
     CDS             complement(449..1525)
                     /gene="trpD"
                     /locus_tag="AC16Y1_01710"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR5831212.1"
                     /translation="MTWKDILGLLSRGQDLSHDQAAWAMEDLMSGNATDSQIAAFAMG
                     LRVKGETPVEITGLVDTMMKYAIQVNLDRFAVDVVGTGGDGSHSVNISTMAALTVAGC
                     GVPVLKHGNRAVSSKSGTADVLEALGVAINMPPILVSSSVAEAGIGFCLAPAHHPAMR
                     YAANVRKELGVPTVFNVLGPLANPGQPQAAVIGVADARLAPTLAQVQLQRGKSSILVR
                     SEDGFDEITTTGPTRAWDVTGGTIREEVLAPSSFGLETSSIDQLRGDDANFNAQVARA
                     IFAGRSDGNLSAIRDVVALNAAAALVAYDSAVSRFQYGTPTESLTARMNRALPAAFGS
                     LSSGAAGSVLDVWISVSQRYALKG"
     gene            complement(1559..1984)
                     /locus_tag="AC16Y1_01715"
     CDS             complement(1559..1984)
                     /locus_tag="AC16Y1_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5831213.1"
                     /translation="MSPSGRTLRVLVYSDNAKVRNTVKTSIGKRPAEDISDVEIYDVA
                     TEPALIKVMDTKQFDLVILDGEAAPAGGLGVCRQLKDEIFNCPPILVLVARVDDRWLA
                     TWSRADAIVAHPIDPRALVNAVIGLIRPTNSSTKTAIAN"
     gene            2225..>2389
                     /locus_tag="AC16Y1_01720"
     CDS             2225..>2389
                     /locus_tag="AC16Y1_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013349008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome oxidase subunit III"
                     /protein_id="HIR5831214.1"
                     /translation="MVSVGTIVWLSSELMFFAALFAMYFTLRAVNLELWAEEVEKLNI
                     PLSAFNTTILV"
BASE COUNT          618 a          577 c          554 g          640 t
ORIGIN      
        1 aattcatgca ccgcgtcaaa gtgcgctgcg ccagtccaag ttgatgtgag tacggttact
       61 tgatcaggtt taaatcggcg aactatttca taaacaaatg tctgtatgcc acctggtcta
      121 ggcgggaaat catttgtaat gaatagtgtg ttcgtcacct gtcataggtt actggcagcc
      181 gcaggtgcat gtggattttc gccatcgccc ttaattaacc gaagcggagc aacaattcca
      241 gcttgagcta aaactgttag tgcatcttta tggtcatcta ttgactcagg tactggccca
      301 aggagcatgg tcactacaca ttcgccgcag gcgatgtctt tcattacaca gctattgcaa
      361 tcaatcaaca tagtgctcag gctaggcggg ggtaccgaca gatttttaat ctgtcgagat
      421 agaaaaaggc agatttttaa gctggaagct agccttttaa ggcgtaccgc tggctgactg
      481 aaatccacac atcaagtacg gagcctgccg caccactaga tagtgacccg aatgcagctg
      541 gcaaggcgcg attcattctg gcagttaacg attcggtcgg tgtgccgtat tggaatcgac
      601 ttaccgcgct gtcgtatgca actagtgcgg ctgctgcatt aagtgcaaca acatctctta
      661 ttgcacttaa atttccatcg ctgcggccag caaaaatggc tcgggcaact tgagcattga
      721 aatttgcatc atcaccacgt agttgatcga ttgatgatgt ttcaagtcca aatgaacttg
      781 gtgctaaaac ttcctcacgg atcgtgcctc cggtaacatc ccatgcccga gttgggccag
      841 ttgtcgttat ttcatcgaaa ccatcttcgc tgcgtaccaa gatcgacgat ttaccgcgtt
      901 gcaattgaac ttgagcaagt gttggggcaa gtctggcatc tgcaacacca attacggcag
      961 cttgtggttg gcctggattg gctagagggc caagaacatt aaataccgtt ggtacaccga
     1021 gttctttgcg cacatttgcg gcataacgca ttgccgggtg atgcgccggt gccaagcaaa
     1081 aaccaatgcc agcttcagca actgaagatg aaactaaaat cggtggcata ttgatcgcga
     1141 cgccaagcgc ttcaagtaca tctgcagtac ctgactttga cgaaactgcg cgattaccgt
     1201 gttttaaaac cggaacgcca cagcccgcaa cagtaagtgc tgccatcgtt gaaatgttta
     1261 ctgaatgcga accgtcgcca ccagtgccaa cgacatcaac tgcaaatcga tctagattta
     1321 cttgaatcgc atacttcatc atggtgtcga ctaagccagt tatttctact ggcgtctcac
     1381 ctttaactcg caatcccatc gcaaaagctg caatttgtga atcggttgca ttgcccgaca
     1441 ttaaatcttc catcgcccaa gcagcttggt catgcgatag gtcttgccca cgtgaaagta
     1501 acccgagaat gtctttccaa gtcacttcga tcaaactttg cccctaattg ggtattggtt
     1561 aattggcgat tgcagtttta gtagaactgt ttgttggtct aattaatccg attactgcat
     1621 taactaatgc gcgcgggtca attgggtgag ccacaattgc atcagcgcga gaccaagtgg
     1681 caagccagcg atcatctact cgagctacta aaactaaaat cggcggacag ttaaaaatct
     1741 catctttaag ttggcggcat acgccaagac caccagcagg cgcagcttct ccatcaagta
     1801 tcactaaatc gaactgctta gtgtccataa cttttattaa ggcaggttcg gtggcaacgt
     1861 catagatctc tacgtcactt atgtcttcgg ccggacgttt accaattgag gtcttgaccg
     1921 tgttgcggac tttagcgttg tcgctgtaga ccagcactcg aagtgttcta cctgaaggac
     1981 tcacgcaata agcgtatccg acggctcaga actgcgaaat ttgtccccga tggggcactt
     2041 ggccagaggg taaatcgtcc actgattcgg ccgatagatc gagataaaat tgccatattt
     2101 tgtgaggtac cagacaccgc caaatcgacc acctgctcaa aatagccttt aatcttcaag
     2161 taaactcggg tttgtgagta caagttcagc agtcagagtc ccatcctccg cagagcgtcc
     2221 gaacatggtt tcggttggca caattgtctg gctttctagt gagttgatgt ttttcgctgc
     2281 tttatttgca atgtacttca ctctgcgcgc ggtgaatttg gagctttggg ccgaagaagt
     2341 tgagaaatta aatattccac tttcagcttt caacacaact attttggtt
//
LOCUS       DBNHVN010000093         5626 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_536239, whole genome shotgun sequence.
ACCESSION   DBNHVN010000093 DBNHVN010000000
VERSION     DBNHVN010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5626)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5626)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5626
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_536239"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1678)
                     /gene="dnaK"
                     /locus_tag="AC16Y1_01725"
     CDS             complement(11..1678)
                     /gene="dnaK"
                     /locus_tag="AC16Y1_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR5831215.1"
                     /translation="MARAVGIDLGTTNSVVSVLEGGEPTVIANAEGSRTTPSVVAFAK
                     NGEVLVGEVAKRQAVTNVDRTIRSVKRHMGNSSWNVDIDGKKYNAQEISARVLQKLKR
                     DAEAYLGETVTDAVITVPAYFNDAQRQATKDAGQIAGLNVLRIVNEPTAAALAYGLDK
                     GEQDQTILVFDLGGGTFDVSLLEIGEGIVEVKATSGDNNLGGDDWDQRIVDHLVTTFK
                     NSNGIDLSADKVALQRLREAAEKAKIELSSSQSTNINLPYITASDAGPLHLDLSLTRA
                     EFQKMTADLLERTKAPFNQVVKDAGVKVSDIHHVVLVGGSTRMPAVTDLVKELTGGRE
                     PNKGVNPDEVVAVGAALQAGVLKGEVKDVLLLDVTPLSLGIETKGGVMTKLIERNTTI
                     PTKRSEIFTTAEDNQPSVLIQVFQGEREMAAYNKKLGTFELTGLPPAPRNVPQIEVTF
                     DIDANGIVHVSAKDLGTGKEQSMTITGGGALSKEEIDRMMADAESHAEEDRKRREEAE
                     IRNQADALVFQTEKFLEENGDKVPADAKSNVEEPLAELKKALEGEDL"
     gene            1858..2895
                     /locus_tag="AC16Y1_01730"
     CDS             1858..2895
                     /locus_tag="AC16Y1_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR5831216.1"
                     /translation="MQAVVFDAFGADLYIGELPLPAPRPHGVTIKVMATGLCRSDWHG
                     WQGHDGDIKELPHVPGHEFAGLIHEVGALVKNWKVGDRVTVPFVCGCGECDDCLSGNA
                     QVCKFQWQPGFNGPGSYAEYVRIPNADFNLVAIPENISFETAASLGCRFATSYRAIVH
                     VAKVQPGEWVAVFGCGGVGLAAVMIAKSRGARVIGIDTSHQARDRAKLAGAEEVLDSV
                     HDDVIGQIKKLTEFGADVTVDALGNKDLVKLAIESLRRLGRHIQIGLLPPTVIGDTAT
                     VPMHLVIGRELQVLGSHGMSAAAYPEMLSDIASGLLRPDSLIERTIKLAEVPDALKNI
                     GSLPGITIIKP"
     gene            2915..4060
                     /locus_tag="AC16Y1_01735"
     CDS             2915..4060
                     /locus_tag="AC16Y1_01735"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIR5831217.1"
                     /translation="MRVTADSLRKLRKRRSVKWRGFSKEVLPLPVAEMDYPIAKQIKE
                     VLIDMVERSDVGYGGIIPELPDAFAKFAKRKWNWQIDPAQFRLATDVGVGGVEVIRVF
                     TNPGDKVVINSPVYHNFYNWIKETKCELVDVPLIYDLDQWSLDTQGLERAFANGAKVF
                     LMCHPHNPVGHVFSRVELQEIAKLAKQYGVIVISDEIHAPLTFREVNFIPYLALNEDA
                     RETGICITSASKTWNLAGLKCAQIITQNNALHKKLDELPVTTPWRASILGAWASYVAY
                     KDGEKWLKAVMKNLDRNRKYLNKLLKKHLPKAKYDPPRSTYLAWVDLTAYELADASAQ
                     LLEKGKVAFNNGKDFGPSGINFVRINLATSKRILRKAIKRAGKALKD"
     gene            4060..4569
                     /locus_tag="AC16Y1_01740"
     CDS             4060..4569
                     /locus_tag="AC16Y1_01740"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019852183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR5831218.1"
                     /translation="MAKIIGYEVKTPWVPMSVLVQVDKQPIVIGAAFLAMPKFLRKAG
                     QKLNVTEFKKDAKLAGVTEAINRWIDGDLTAFNKLKVHQPGGEFMQDCWKELRKIKGG
                     TVVSYAQLAKSAGRPLALRAAGTACATNLIAPIIPCHRVIKTGGELGNYGFGLPLKAK
                     LLEHEGVEL"
     gene            complement(4566..>5626)
                     /locus_tag="AC16Y1_01745"
     CDS             complement(4566..>5626)
                     /locus_tag="AC16Y1_01745"
                     /inference="COORDINATES: protein motif:HMM:NF022449.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIR5831219.1"
                     /translation="KFAQKKGYSRHKNPKALYDEDDALQAIKLFKICKFDQKINVENK
                     ASLQFHHSGHILGSAFVSLEVSTKKFLFTGDLGRPTHPVLVSPDEFPADQFDAVITES
                     TYGDRTHEKPTGEFAKVINQTISRGGIVLIPAFAVDRTEVVLVELRELMESGAIPKVP
                     IFVDSPMALTALDFYREALNAGRSEIRTDVIQEWRGLDPFDPGTLEERRSVEDSKSLN
                     NQQTPCIIVSASGMATGGRVVHHLARLLPDSKNTVLLVGYQAQGTRGRQLEDGDKRIR
                     IFGEDIPVRAQIAKADTFSVHADGDELINWLKTAKQPGQVFVVHGEIESAEIFAKRIK
                     SDLGWNAIVPEPEQTFEV"
BASE COUNT         1551 a         1220 c         1239 g         1616 t
ORIGIN      
        1 tcttgatggc ttataggtct tcaccttcga gcgctttttt caactctgct agcggttctt
       61 caacgtttga ctttgcatca gctggtacct tgtcgccatt ttcttccaag aacttttcag
      121 tttggaaaac taatgcatcg gcctgatttc gaatctcagc ttcttcacga cgcttacgat
      181 cttcttcagc atgtgattct gcgtcagcca tcatgcgatc aatctcttct ttagaaagag
      241 cgccaccgcc agtgatagtc atagattgtt ctttccctgt tcccagatcc ttggctgaaa
      301 catgcacaat accgtttgca tcaatatcga aagttacttc gatttgagga acgttacgtg
      361 gtgctggtgg cagaccagtt aactcgaatg tgcctagctt tttgttatag gcagccatct
      421 ctcgttcacc ttggaacacc tgaattaaca ctgacggctg attatcttca gcagttgtga
      481 aaatttctga gcgcttcgtt ggaatagttg tgttgcgttc gatcaacttg gtcataacgc
      541 cacccttggt ttcgatacca agtgaaagag gagttacgtc aagaagtaga acgtctttga
      601 cttcaccctt aagtacacca gcttgaagag cagcacctac ggctacaact tcatctgggt
      661 taacaccctt attcggttca cggccgcctg ttaattcctt aactaaatca gttacagctg
      721 gcatacgtgt tgatccacca acaagtacca cgtgatgaat gtcagaaact tttacgccag
      781 catctttaac aacctggtta aacggtgctt tagtgcgttc aagcaaatca gctgtcatct
      841 tttggaattc agcgcgagtc aaacttagat ctaagtgcag tggaccagca tcagatgccg
      901 tgatgtaagg caagttgatg ttagttgact gtgaagacga aagttcaatc ttggcttttt
      961 cggctgcttc acgtagacgt tgtaacgcta ctttgtctgc agataagtcg ataccatttg
     1021 aattcttgaa agtagttact aagtgatcga caatacgttg atcccagtca tctccaccaa
     1081 gattgttatc accactagtg gctttaactt caacaatgcc ttcaccaatc tcaagaagtg
     1141 aaacgtcgaa agtaccgcca cctaagtcga ataccaaaat agtttgatct tgctcacctt
     1201 tgtctagacc atatgcaagc gcagctgcag ttggttcgtt aacaatacga agcacattaa
     1261 gaccggcaat ctggccagca tctttagtgg cttgacgttg agcgtcatta aagtaagccg
     1321 gaactgtgat tactgcatca gttacagttt cacctaagta agcttcagcg tcccgcttta
     1381 atttttgtag cacgcgagca gaaatttctt gcgcgttata tttctttcca tcaatgtcga
     1441 cattccatga gctattaccc atgtggcgct ttactgagcg aattgtgcga tcaacgtttg
     1501 ttaccgcctg acgcttagcg acttcgccga ctaaaacttc accatttttt gcaaaggcca
     1561 ccaccgatgg ggtggtacga gaaccttctg catttgcgat cacagttggt tcgccgcctt
     1621 ctaggaccga gaccaccgag ttagtagttc ccagatcgat acctactgca cgagccattc
     1681 tttttcctcc atagagttac tagttcgcct tgctgacccg cacctggcac ctgaaacctt
     1741 ggttatcaga taccttgagt cgagtcggct caaggacaaa tatcataacc caaaaggctg
     1801 aaagtctatt cccggccaat ttctgtggat atttattggt tctactagcc ttaatccatg
     1861 caagccgtag tttttgatgc ttttggagct gacctatata taggtgaact accgctgccg
     1921 gcacccaggc cacatggtgt gacgatcaaa gttatggcaa ctggcttatg ccgaagtgac
     1981 tggcatggct ggcaaggtca tgacggcgat atcaaggaac ttccacatgt tccagggcat
     2041 gagtttgccg ggctaatcca tgaagtcggt gctttggtta aaaactggaa agttggcgac
     2101 cgcgtaacag tgccgttcgt atgtggttgc ggcgaatgtg acgactgttt atcgggtaac
     2161 gcccaagttt gtaaatttca atggcaacca ggatttaacg gacctggttc atacgcagaa
     2221 tacgtgcgca tcccaaatgc agattttaat ttagtagcta tcccagaaaa catttccttt
     2281 gaaacggcgg cttcgttagg ttgtcgcttt gccacttcgt atcgagctat tgttcatgtc
     2341 gcgaaagttc agcctggtga atgggttgca gtatttggtt gcggtggcgt cggattagct
     2401 gccgtaatga ttgctaaatc tcgcggcgct cgagtaatcg ggattgatac ttcgcatcaa
     2461 gcacgtgatc gagcaaagtt agccggcgct gaggaggtac ttgattccgt acacgatgat
     2521 gtaattggcc aaatcaaaaa gttaaccgaa tttggcgcgg atgtaaccgt tgatgcatta
     2581 gggaacaaag atttagttaa gttagcaatt gagtcacttc gacgacttgg tagacatatt
     2641 caaattggcc ttttgcctcc tacggttatt ggtgacactg caacagtgcc aatgcattta
     2701 gttattgggc gtgaattaca agtcttaggc agccacggca tgtcagcagc tgcttaccca
     2761 gaaatgctga gtgacatagc aagtggctta ctcagaccag attccctgat tgaacgcacg
     2821 attaaattag cggaagtacc ggatgcgcta aagaatattg gttctttgcc gggcatcaca
     2881 attattaaac cgtagtagtt gctaacctgt gggtgtgaga gttaccgctg attcactgcg
     2941 caaattacga aaaagaagaa gtgttaagtg gcgaggattt agtaaagaag tgttaccgct
     3001 tccggttgca gagatggact acccaatcgc caagcaaatt aaagaagttc tcattgacat
     3061 ggttgagcgt tcagatgttg gctacggcgg aataattcca gaacttccag atgcatttgc
     3121 caaatttgca aaacgcaaat ggaactggca aattgatccg gcacaatttc ggttagccac
     3181 cgatgttgga gtgggcggcg tcgaagtaat tagagttttc acaaatcctg gcgacaaagt
     3241 ggtaatcaat tcgccggttt atcacaattt ctataactgg attaaagaaa ccaagtgcga
     3301 actagttgat gtgccactta tttatgatct tgaccagtgg tcacttgata cccaaggact
     3361 cgaaagagca ttcgcaaacg gggcaaaagt atttttaatg tgccatccac ataatccagt
     3421 cggacatgtg ttttctcgag ttgaacttca agagattgca aagttggcaa aacaatatgg
     3481 tgtgatcgtg atctctgatg aaattcacgc acctttaact ttccgcgaag taaatttcat
     3541 tccatatctt gcacttaatg aagatgcacg agaaacaggt atttgtatta cttcagcaag
     3601 taaaacttgg aatcttgctg gtcttaagtg tgcgcaaata attacccaga ataatgctct
     3661 tcataaaaaa ctagatgaat tgccagtaac caccccatgg cgtgcttcaa ttttgggcgc
     3721 ttgggctagt tatgtcgcct ataaagatgg cgaaaagtgg ctaaaagcag tgatgaaaaa
     3781 tctagatcga aaccgtaaat atttaaacaa gttactcaag aagcacttac caaaagctaa
     3841 atacgatcca ccgcgaagta cttatttagc ttgggttgat ttaaccgctt atgaactggc
     3901 ggatgcctct gcgcaacttt tagaaaaagg caaggttgcc tttaataacg gcaaagattt
     3961 tggaccaagt gggattaact ttgttcgaat taacttagct acgagtaaga gaattctgcg
     4021 caaagcaata aagcgcgctg gaaaggcact taaagattaa tggccaaaat tattggttac
     4081 gaggtaaaaa caccttgggt gccgatgtcg gttttggttc aagttgataa acaaccgata
     4141 gtcatcggtg cggcatttct tgcgatgcca aagtttttgc gaaaagccgg acagaaatta
     4201 aatgtgactg aatttaaaaa agatgcaaaa cttgccggcg tcaccgaagc aattaatcgc
     4261 tggattgacg gtgacttaac tgctttcaat aaattaaaag ttcatcaacc gggtggtgaa
     4321 ttcatgcaag attgctggaa agaactgcgc aaaataaaag gtgggaccgt agttagttat
     4381 gcgcagctcg ctaaatctgc tgggcgcccg ttggctttgc gtgctgcggg tacagcctgc
     4441 gcaaccaatt tgattgcgcc aattattccc tgccaccgag ttataaaaac tggcggtgaa
     4501 cttggtaatt atggttttgg tttaccgctg aaagctaagt tgcttgaaca tgaaggtgta
     4561 gaactttaaa cttcaaaagt ttgctctggc tcgggaacaa ttgcattcca accaagatca
     4621 gatttaatac gtttggcaaa aatttcagcc gactcaattt caccatgcac cacaaaaacc
     4681 tgaccaggtt gttttgccgt tttaagccag ttaattaact cgtcaccatc agcatgcact
     4741 gaaaatgtat ctgctttagc aatctgtgct ctaacgggaa tatcttcgcc aaaaattcga
     4801 attcttttat cgccatcttc caattgccga ccgcgagtac cttgcgcttg gtagccaact
     4861 agtagcacgg tgttttttga atctggcagc aatctagcta agtgatgaac aactcggccg
     4921 ccagttgcca tgccactggc cgaaacaata atgcaaggtg tttgctggtt attcaaggat
     4981 ttggagtctt caacactacg tcgttcttct agtgtgcctg ggtcaaacgg atcaagaccg
     5041 cgccattctt gtattacatc agttctgatt tcactgcgac cggcattaag tgcttcgcga
     5101 taaaaatcga gtgcggttaa tgccatcggt gaatctacaa aaatagggac ttttggaatt
     5161 gctcctgatt ccatcaactc tcgaagttca accaatacaa cttcagttcg atctactgca
     5221 aatgcaggaa ttaatacaat tccaccgcga ctaatagttt gatttataac ttttgcaaac
     5281 tcacccgttg gcttttcgtg ggttcgatca ccataggttg attcagttat cactgcatca
     5341 aattgatcag ctggaaattc atctggcgaa actagaactg gatgtgtagg tctacctagg
     5401 tcgccagtga atagaaattt cttagtcgaa acttctaatg aaacaaaagc tgaacctaaa
     5461 atgtgcccag aatgatgaaa ctgcaaacta gctttattct caacatttat tttctgatca
     5521 aatttacaaa ttttaaaaag ttttatcgcc tgtagagcat cgtcttcgtc ataaagtgct
     5581 tttggattct tatgtcggct ataacctttt ttctgcgcaa attttg
//
LOCUS       DBNHVN010000094        11628 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1354065, whole genome shotgun sequence.
ACCESSION   DBNHVN010000094 DBNHVN010000000
VERSION     DBNHVN010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 11628)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11628)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11628
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1354065"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="AC16Y1_01750"
     CDS             <1..279
                     /locus_tag="AC16Y1_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR5831220.1"
                     /translation="AKEFSNESVQFIGLNTRDSLPSAIAFNERFATGFPSIQDKEGEL
                     TLAFGKLGPAATPTTIVLDKSGQPAGRILGVITEAQLRNLIKVILEEA"
     gene            282..1049
                     /locus_tag="AC16Y1_01755"
     CDS             282..1049
                     /locus_tag="AC16Y1_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HIR5831221.1"
                     /translation="MQLVSGVTEIVSNGSLLLAFPLAFAAGVISFLSPCVLPLVPGYL
                     SYAAGIAGARANSKRTRLTRSRTFWGTLLFVFGFSIVFVSFGALFGAAGQFFIANQKI
                     IQTLSGVLIIVMGLGFLGLIPLLQREVRLHRVPTGTLVGALIMGITFGIGWTPCIGPT
                     LAAVQSLAFSEANAQRGALLSAAFSFGLGLPFLVIGLMLERGVKAVSALRKHSKLIMQ
                     IGGGLLLIIGLLLVTGLWNELTIVLRVWASNWTGWLL"
     gene            1094..2527
                     /gene="resB"
                     /locus_tag="AC16Y1_01760"
     CDS             1094..2527
                     /gene="resB"
                     /locus_tag="AC16Y1_01760"
                     /inference="COORDINATES: protein motif:HMM:NF016995.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HIR5831222.1"
                     /translation="MRTALILLFLLALASVPGSIFPQRGTSPLRVNQFLRDNPEVGAV
                     LDRFYMFDVFASPWFGAIYLLLFISLAGCVIPRLKVHWEEIRSLPPEAPKNLNRLPVY
                     ESFEVTKRKEDLLNQTEVAWKKSGWRVRRGSDWISAERGYSRETGNLLFHLALLVVLV
                     AVGFGSATGYRGTVIVKEGSGFANNVTQYDTFTPGRNFDSANLPPFSFKLTEFESDFQ
                     RGGMQNGASRMFLADVTYKENLSSSEKQTRIEVNEPLRISGSAIYLVGHGYAPEFVIK
                     NSSDEIIWQDTAVFLPQDSGFTSTGVIKIPDTTPQIGIQGFFLPTVTTDFSNGPISTF
                     PAPDNPAAYLSLWVGDLGLDSGLPQSVYRLETANMQRLGIEELKPGETWQLPQGYGTL
                     EFVGFKQWASFSISADSGKGPALLASILAIVGLTMSLLIPRRRAWLRVSDTSHLEVTI
                     CEVAALAKTEAPGLRDEVSKLAIRVKE"
     gene            2550..3545
                     /gene="ccsB"
                     /locus_tag="AC16Y1_01765"
     CDS             2550..3545
                     /gene="ccsB"
                     /locus_tag="AC16Y1_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HIR5831223.1"
                     /translation="MSVNETLAEVSDLLVYASMVTLTIAMAAFSVALAKGRRLESLED
                     SAIRTTSVSASARLGSTLTLETPSVSTSVESGAGRRSGNIGMAVMWLTTLLLGASLIT
                     RGVSAKRLPWGNMYEFSLAAGFAVVAAYCFLSIKRDLRWFGLFISIPVLLDLGLAVTV
                     LYTESAPLVPALRSYWLAIHVTAAIVCVGAFTVGAALATLYLVAENAESRVALNGAPK
                     RFDGIANRLPSADRLDLMSYRIHAFMFPIWTFSVIAGAIWAEAAWGRYWGWDPKETWA
                     FITWVVYAAYLHARATAGWRGRKAAYVAIAGYLTILFNYFVVNIFIQGLHSYAGV"
     gene            complement(3547..4428)
                     /locus_tag="AC16Y1_01770"
     CDS             complement(3547..4428)
                     /locus_tag="AC16Y1_01770"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019814985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HIR5831224.1"
                     /translation="MATREQWIQGARPLTLPAAISPVLIGTAIAKYEGGLNLIVAFFA
                     LIVAIALQVGVNYANDYSDGVRGTDDDRVGPVRLVGQGLVPATEVKKAAFISFGVAAV
                     SGLIMMALSGLWILLPIGLAAIWSAWFYTGGKNPYGYAGLGELFVFVFFGLAAVMGTT
                     SAQTGNYSWVSLFYGISCGALSAAILVANNLRDIHTDLQSAKKTLAVRLGDRRTRELY
                     KALIAVAFFMPVIISFRGDGPMYAYVSLFAILSARYPLHLIQTGKTGNELIPVLTFTG
                     RLLLVFAVISSIAISFS"
     gene            complement(4429..5730)
                     /locus_tag="AC16Y1_01775"
     CDS             complement(4429..5730)
                     /locus_tag="AC16Y1_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5831225.1"
                     /translation="MMQSDISGDFANPRSLVAVQIPPGAGGVNRLAPMLTSALDGTGP
                     AIAPIPTSPTGLVNLLTDAIKPEDKRFPLESSDIALVCATSGSTGNPRGVLLSANAIR
                     YSVQAFADRFGGDYRWVMSVPAHRIAGVMVLARAALNGNPYLIDPSIGGARVFDAATF
                     SVTTANAVRESKKDGRPIAVSLVPTQIGRLLDSGSVGIEALQAYKLVLSGGTATPQPM
                     LNRLRELGINVVISYGMTETCGGCVFDGKPLTGVTISLGTKDDVEPGRISIAGPVLAS
                     GYRLRPDLAAVEFIENKLITHDVGKLDSADLLHVLGRLDDVVQVGGTNVSLSAVEALL
                     RHHPAISDVAVIDIADDLWGSIPMAYVTLRDQQLNKQDLITALKNIVMERIGRAATPR
                     TIQFVNQLPMLDSGKIDRLTLRLQASEEISSGKITHPGIGK"
     gene            5794..6726
                     /gene="menB"
                     /locus_tag="AC16Y1_01780"
     CDS             5794..6726
                     /gene="menB"
                     /locus_tag="AC16Y1_01780"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HIR5831226.1"
                     /translation="MDTRNKYVLPPVVADDSPAKSDPGYSSIRLACDWKSIGSYQDIK
                     LDVSTGNDSGIAKITINRPEKRNAFRPQTVKELIQAFDVVRDDPSIGVVILTGQGDWA
                     FCSGGDQMIRGDDGYIGDDEIAQAGVGRLNVLDLQIAIRRIPKPVIAMVAGYSIGGGH
                     VLHVVCDLTIAAENARFGQTGPKVGSFDGGYGSGLLARHIGQKRAREIWYTCRQYGAE
                     QAYDWGLVNQVVPLVDLEHETVKWCKEMLELSPLALRMLKASHNAADDGLAGVQQLAG
                     DATLLFYMTEEAQEGRNAYREKRRPDFKKFPKRP"
     gene            6742..7782
                     /locus_tag="AC16Y1_01785"
     CDS             6742..7782
                     /locus_tag="AC16Y1_01785"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:NF002782.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HIR5831227.1"
                     /translation="MSIENLAKDAKAFALPLANRFRGIDVRYGLVFAGSAGWAEFAPF
                     DEYDDEVSGRWLAGALEQAFGDFPDRVRDFVPVNAIIPAVDPTTAAAEAFKAVVEYGM
                     TTLKVKVAEPSQDFADDLARLTAIRAQLNDLAVTEVNIRIDVNARWTVQDAIDLIPKY
                     ELAAGGLDYVEQPCATLAQCAQVKEQTSVKIAIDEGLRLAKRIDPQAIRNSADVLIVK
                     SIPQGGVRNSLLAIADIGLPVVVSGSLDTSIGLTSGLALACSVSDLAGACGLGTGLLF
                     ANDLVKPKTLPRDGAIYFSQPSPDLQLLEQANLAVEVSVQKAWQERMVRAWQASGSKL
                     VSEKVKQAVENW"
     gene            7779..9401
                     /gene="menD"
                     /locus_tag="AC16Y1_01790"
     CDS             7779..9401
                     /gene="menD"
                     /locus_tag="AC16Y1_01790"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HIR5831228.1"
                     /translation="MNPSTQLALEISKSILACGVTDVVVCPGSRSAPLAWQMAQLASK
                     QKIKLHTRIDEREAGFFALGIAKATKRPVPVIVTSGTAVANLLPAVVEAYHSGIPLVV
                     LSADRPAAVRGNSAPQTTNQVGIFSSFVKAFADTAVPDPEILAAIKSSLQVHPGPVQI
                     NAQFDMPLMPEDPEIDFGQISEIEFIKEVQQKEKLELPSKGLLVIGDLPDGQHVQELI
                     DLAEDCGYPIIWEPTAQVMRSKNAIDHGALLLQSDKLPKPEVVITAGLIGLSRSVLNV
                     LKTTPHHIAIHLSATGAQLPNPVLSAKQTLTAVPEIKTLVDKDWLAKWQQAANHASDV
                     ISKKIKLETLTGPSAAVTLWNHVPADSTLFISSSWSARHIEMYAQARPDLKTFGNRGV
                     NGIDGLISTAAGVALTQQNRTYLLIGDIAFLHGMGGLNISAENEIPNLTVVVLDNDGS
                     GIFSQLEQGAAKYQKHFEQIFGTPHGKDLWVIAESLGVPATRVTTKSELLKALALSDK
                     IPGLQVIVCSTGSRKDEQELINLISAQVSDSI"
     gene            9467..10324
                     /locus_tag="AC16Y1_01795"
     CDS             9467..10324
                     /locus_tag="AC16Y1_01795"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecB family exonuclease"
                     /protein_id="HIR5831229.1"
                     /translation="MADKFLKVEVEQPENAKLPKRVSFSQMSLFQQCGLKYFFSYIDN
                     WREPPTAALAGGSITHDVIERLYRLPNEERTLAKAVEILREYGPKFLAQPEYQKFAED
                     LNMKHSVRDAVENLFAVEDPKSLVVQPEHLEMELNVDINGVNFFGKVDRFTQDGANRV
                     TDYKTGKSPGKFVDGKLAQPYLYALGYKLQHDIDVDEVELIYLNAKEVIRRPAEWGQI
                     TKVGEDLATMRNQSQASLNESAWDAKVSKLCDYCSFQEVCKARNPDAPLPGSIKSDEK
                     LYSIGLIQK"
     gene            complement(10328..>11628)
                     /locus_tag="AC16Y1_01800"
     CDS             complement(10328..>11628)
                     /locus_tag="AC16Y1_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5831230.1"
                     /translation="TDAHTKLPKDVLAKERQVVMSRALSLNFKEPIEIVSGQGAYLYD
                     TAGKPYLDLVNNVAHVGHCHPRVVEAGSKQMATLNTNTRYLNQNAIEYARALASTMPD
                     PLNVVFFVNSGSEANDLALRLAQAHTKSKGMIVLDQAYHGHVTSIIDISPYKFSGKGG
                     LGRPDHVRVVATPDSYKGLHRGAGSGEKYATEFAAQLTDLNQPLCAFISESIISTAGQ
                     ITLAPGFLPLAYEQTRAAGGVCIADEVQIGMGRVGECFWGFELHNVVPDIVTIGKPIG
                     NGHPLAAVVTTPEIAHSFLTGMEYFNTFGGNPVSAAIGQAVLDVVADQALQANANHLG
                     NYLMDSVRQLGKSIPIIGDVRGSGLFIGVEFVLDQETQVPATQLTADLIEYAKIQGVF
                     LSSDGPANNVLKIKPPLVITKEDVDLFLTVLKDGLTKLTN"
BASE COUNT         3280 a         2443 c         2653 g         3252 t
ORIGIN      
        1 gcaaaagaat tctctaatga aagtgtgcaa ttcatcggct taaatactcg ggatagtttg
       61 ccatccgcaa ttgcctttaa tgaaagattt gctactggct ttccttcgat tcaagacaaa
      121 gaaggcgaac ttactttagc ttttggaaaa cttggtccgg ctgcaactcc gacgacaatt
      181 gtgttggata aatcgggtca accagctggt cgaattttag gcgtgataac tgaagcccag
      241 cttcgcaact taatcaaagt aatactcgag gaagcgtagt catgcagcta gtttcgggtg
      301 tgacggaaat tgtaagtaac ggttcactgt tacttgcatt tccacttgct tttgcggcag
      361 gtgtaatttc ttttttgtca ccatgtgtgc ttccactggt gccggggtat ctttcgtatg
      421 cagctggaat cgctggtgct cgggctaatt caaaacgcac tagattaact cgatcgcgga
      481 cattttgggg aacactgctt tttgtttttg gattttcgat tgtgttcgtt tcatttggcg
      541 cactctttgg tgcagctgga caattcttta ttgctaatca aaaaattatt caaaccctaa
      601 gcggcgtatt gatcattgtg atgggcttag gttttctagg tttaattcca cttctgcagc
      661 gcgaagttcg actacatcga gtaccaactg gcacattagt tggcgcccta atcatgggaa
      721 taacttttgg cattggttgg acgccgtgta tcggcccaac tttagctgcc gttcaaagtt
      781 tggctttttc ggaagctaat gcgcagcgcg gtgcactgtt gagtgctgct ttttcttttg
      841 gacttggtct gccgttttta gtgattggct tgatgttaga acgcggtgtt aaagccgtta
      901 gcgcattgcg caaacattca aaattaatta tgcaaattgg tggcggatta cttttgatta
      961 ttggtttgct actagtcact ggcttgtgga atgaactaac aattgtgctg cgcgtttggg
     1021 caagtaactg gactggttgg ttgctgtgaa attagctgat aacttccgct gggtttggcg
     1081 tcaattaact agcatgcgca cagcgttaat tttgcttttt ttactggcgt tggcgagtgt
     1141 gcccggttca atttttccgc agcgcggaac ctcgccactt cgagtaaatc aatttttgcg
     1201 agataaccct gaggtaggcg ctgttttaga tcgtttctac atgtttgatg tttttgcctc
     1261 gccatggttc ggtgcgattt atttactttt atttatttcg ttagctggtt gtgtaattcc
     1321 ccgcttaaaa gttcattggg aagaaataag aagtttgccg cccgaagcac caaagaactt
     1381 aaatcgactt ccggtttatg aatcatttga agtaactaaa agaaaagaag atttacttaa
     1441 tcaaactgaa gtggcttgga aaaaatctgg ttggcgtgtt cgccgaggat cagattggat
     1501 ctcagccgaa cgcgggtatt cgcgtgaaac tgggaatcta ctgttccacc ttgctttact
     1561 tgttgtttta gttgcagttg gttttggtag tgcaaccggt taccgcggaa cggtaattgt
     1621 taaagaaggt tcgggttttg caaacaatgt aacgcaatat gacacattca ctccgggtag
     1681 aaattttgat tcggcaaact taccgccatt ttcatttaag ttaactgaat tcgaatctga
     1741 ttttcaacga ggtggaatgc aaaatggagc atctcgcatg tttttggcgg atgttacata
     1801 caaagaaaat ttatctagct ctgaaaagca aactcgaatc gaagttaatg aaccattgcg
     1861 catttctggt tcagcaatat atttagttgg acatgggtat gcaccagaat ttgtaatcaa
     1921 aaattcaagt gatgaaatta tttggcaaga tactgcagta tttttgccac aggattctgg
     1981 atttacctca accggagtaa ttaaaatccc tgatacaacg ccgcaaatag gtattcaagg
     2041 tttcttttta ccgacagtga caactgactt ttcaaacgga cctatatcaa cttttccagc
     2101 accagataat cccgcagctt acttaagtct ctgggttggt gatctcggct tagatagtgg
     2161 cttaccacag agtgtttatc ggttagaaac cgcaaacatg caacgtcttg gaatcgaaga
     2221 attgaagcca ggtgaaactt ggcagttgcc ccaaggttat ggaactttag aatttgtggg
     2281 ctttaagcag tgggcttcat tttcaatttc agccgacagc gggaaaggcc ccgcactatt
     2341 agcctcaata cttgccattg ttggtttaac tatgtcttta ctcattccac gtagaagagc
     2401 ttggcttcga gtgagtgata cgtcacattt agaagtaaca atttgcgaag ttgctgcatt
     2461 agcaaagact gaagctccag gccttcgaga tgaagtttca aaattagcaa ttagagttaa
     2521 agaataagaa tttgaatgca ggagtttaga tgtcagtaaa cgaaacgcta gccgaagtca
     2581 gtgacttatt ggtctacgcg tcgatggtca ccctgacaat cgcaatggct gcattcagtg
     2641 ttgcattggc taaaggtcgt cgacttgaat cacttgaaga ttcagcaatt cgcacaacct
     2701 ctgtctcagc cagtgcccgc cttggttcaa cgctgacact agagactccc tcggtttcaa
     2761 cttcagttga atcgggagca ggacgtcgtt ctggaaatat cggcatggcg gtgatgtggc
     2821 taaccacctt gctactaggc gcatctttaa tcacacgagg tgtttccgca aaacgtttac
     2881 cttggggaaa catgtacgag ttttcgcttg cagctggatt cgcagtcgta gcagcttatt
     2941 gttttctttc gataaaacgt gatttacgtt ggtttggact attcataagt attccggttc
     3001 tgctcgattt aggtttggca gtcactgtgc tctacaccga gagcgctccg ctagtaccag
     3061 cattgcgttc atactggtta gcgattcacg taaccgcagc aatagtttgt gttggagctt
     3121 tcacagttgg cgcagcatta gcaactcttt atctagtagc agagaacgca gaatcgcgcg
     3181 tggcacttaa tggtgcacca aaacgattcg acggcattgc aaatcgctta ccaagtgctg
     3241 atcgtttaga tctaatgtca tatcgaattc atgcattcat gtttccaatt tggaccttct
     3301 cagtaattgc tggagcaatc tgggcagagg ctgcatgggg ccgctattgg ggctgggatc
     3361 caaaggaaac ttgggcattt attacctggg ttgtgtacgc ggcttactta catgcccgcg
     3421 caaccgcggg ttggcgaggt cgtaaggctg cgtacgttgc tatcgctgga tacttaacta
     3481 ttttgtttaa ttattttgtg gtgaatattt ttatccaagg tttacattct tacgcaggtg
     3541 tttaagttaa gaaaaactaa ttgcgatact gctaattact gcaaaaacta gcaacaaccg
     3601 gccagtaaat gttaaaactg gaattaattc attgccggtt tttccagtct gaattaagtg
     3661 cagtgggtag cgagccgaaa gtattgcgaa caaactaacg taggcataca tcgggccatc
     3721 accgcgaaat gaaattatta ccggcataaa aaatgcaaca gcgattaacg ctttgtataa
     3781 ctcgcgggtc cgtcgatcgc caaggcgtac cgcaagggtt ttctttgcac tttgcaagtc
     3841 tgtgtggatg tcacgcagat tatttgctac cagaattgca gctgatagcg cgccgcagga
     3901 aattccataa aacagtgaca cccacgagta attacccgtt tgtgctgaag tagtacccat
     3961 tacggcagct aaaccaaaaa atacaaaaac aaataattca ccaagtccgg catagccata
     4021 aggatttttg ccaccggtat agaaccaagc agaccaaatg gcggctaagc caatcggtaa
     4081 caaaatccat agcccactta gcgccatcat gattaaaccg gaaactgcgg caacaccaaa
     4141 agaaatgaaa gctgcttttt taacttcagt tgcaggaact aaaccttggc caactaatct
     4201 gactggacca acacggtcat cgtcggtacc gcggacaccg tcactgtaat cattggcgta
     4261 attcacgcca acttgcagtg ctattgcgac tattagcgca aaaaaagcaa ctattaaatt
     4321 aagtccaccc tcgtactttg cgatagctgt tccgattaaa actggagaaa ttgcagccgg
     4381 aagtgtgagt ggccgtgccc cttgaatcca ttgctctcga gtagccattt actttccaat
     4441 tcctgggtgg gttatttttc ctgaagatat ttcttcgctg gcttgcaatc gtaacgttaa
     4501 ccgatcaatt tttccggaat caagcattgg aagttgattt acaaattgaa tagttcttgg
     4561 tgttgcagct cggccaattc gttccatcac gatgtttttc aatgccgtga ttaaatcttg
     4621 tttatttaat tgctgatctc gtaacgtaac gtatgccatt gggattgatc cccataagtc
     4681 atccgctata tctataactg caacatctga aattgccgga tgatgacgaa gtaacgcttc
     4741 aacggccgag agtgaaacat ttgttccgcc tacctgtact acatcatcaa ggcggcccaa
     4801 cacatggagt aagtcggctg aatctaattt gccgacatca tgagtaatta atttattttc
     4861 aataaattca actgcagcta aatcgggacg taatcgatat cctgacgcaa gaactggtcc
     4921 agcgatggat attcgacccg gctctacatc gtccttagtt cccagcgaga tagttactcc
     4981 ggtcaacggc tttccatcaa atacacagcc accgcaagtt tccgtcatgc cataagagat
     5041 aacaacattt atgccaagtt cgcgtaatcg attgagcatt ggttgtggag ttgcagtacc
     5101 accacttaaa accaatttgt atgcctgtaa tgcctcgatg ccaaccgaac cagaatcaag
     5161 tagtcgccca atttgggtag gtacaagtga gacggctatt ggtcgaccat cttttttact
     5221 ttcgcgcacc gcatttgcag ttgttacgga aaaagtggcc gcgtcaaaaa cccgtgcacc
     5281 gccaatggat ggatcaatta agtaaggatt tccattgagc gcagctcgtg ctaaaaccat
     5341 aactccggca atgcggtgtg ccggtacaga cataacccat cggtagtcgc caccgaaacg
     5401 atcagcaaaa gcttgaactg aatatctgat tgcatttgcg gaaagtaaaa caccacgagg
     5461 atttccagtt gatccagaag tagcacaaac tagagcgatg tcactggatt ccaatggaaa
     5521 tcttttatct tctggcttga tcgcatcagt taagaggttg actaaaccag tgggtgaagt
     5581 tggtataggc gcgatcgctg gaccagtacc atcaagtgct gatgtaagca ttggtgcaag
     5641 ccgatttacc ccacctgcac ccggcggaat ttgaaccgca acaagtgatc tggggttagc
     5701 aaaatcgccg gatatgtcag attgcatcat tatgactagc ctagagcgag agttaaaaca
     5761 tcaatcaaaa aggcacaggt aaaaggacct taaatggata cccgcaacaa atatgtactg
     5821 cccccagttg ttgctgatga cagtccggca aaatctgacc ctggttactc gtcgatcaga
     5881 ttggcttgtg attggaaatc aattggcagt taccaagaca taaaacttga cgtttcaacc
     5941 ggcaacgatt ctggaattgc aaaaatcaca atcaatcgtc cggaaaaacg aaatgccttt
     6001 agaccacaaa cggtaaaaga attaatccaa gcatttgatg tagtgcgcga cgacccaagt
     6061 attggtgttg taattcttac cggccaaggc gattgggcat tctgtagcgg cggcgatcaa
     6121 atgattcgcg gcgatgacgg ctacatcggc gatgatgaga ttgctcaggc aggcgtaggc
     6181 agactaaacg ttctcgattt acaaattgca attagacgca ttcctaagcc ggtaatcgcg
     6241 atggttgctg gttactcaat cggtggcggc cacgttctac atgtggtttg cgatttaact
     6301 attgcagcag aaaatgcgcg cttcggacaa actggaccaa aagttggatc ttttgatggt
     6361 ggatatggtt caggtctact agctcgacac attgggcaaa agagagctcg tgaaatttgg
     6421 tacacctgtc gccaatacgg tgcagaacaa gcgtatgact ggggattagt aaaccaagtg
     6481 gtcccgttag ttgacctaga acatgaaacc gttaagtggt gcaaagagat gctagaactt
     6541 tcaccactgg cacttcgtat gttaaaagct agtcacaatg ctgcagatga tggtctggcc
     6601 ggcgttcagc aacttgctgg cgatgcgacg ttgctgttct acatgactga agaagcgcaa
     6661 gaaggtcgta acgcctatag agaaaagcgc cgacctgatt tcaaaaaatt tccaaaacgt
     6721 ccgtagttaa gttgctttcg gatgtcaatt gaaaatttag ctaaagatgc gaaagcgttt
     6781 gctttaccgt tagctaatcg ctttcgaggt attgacgttc ggtatggatt agtttttgca
     6841 ggcagtgctg gttgggctga atttgcccct tttgatgaat acgatgatga agtttccggt
     6901 cgctggcttg caggtgctct tgaacaagcg tttggtgatt ttcccgatcg agttagagac
     6961 ttcgttccag taaatgcaat tattcctgct gtcgatccaa cgactgcagc tgcagaagct
     7021 tttaaagcag ttgttgaata tggaatgaca actctaaaag taaaagttgc agagccgagc
     7081 caagattttg ctgacgactt ggctcggcta actgccatcc gtgcgcaact taatgatcta
     7141 gcagttactg aagtgaatat tcgcattgat gtaaatgcca gatggactgt acaagacgca
     7201 attgacctta ttcctaagta tgaattagcg gctgggggat tagattatgt cgaacagcct
     7261 tgtgcaactt tggcgcaatg tgcgcaagtt aaggaacaaa cttcagtcaa gattgcgatc
     7321 gatgaaggct tacggctagc caaacgaata gatccgcaag caattcgaaa tagtgctgac
     7381 gtgttgatag ttaaatccat tccacaaggt ggggtgagaa attcacttct tgcaatcgcc
     7441 gatataggtt tgccagtagt agtcagtggc agcttagata cctcaatcgg attaaccagt
     7501 ggacttgcat tagcttgtag cgtttctgat ttagcggggg catgtggact gggcactggg
     7561 cttttgtttg cgaatgattt agtcaaacca aaaacattac cgcgtgatgg tgcaatttac
     7621 ttttcccaac caagcccaga tctgcaactc cttgaacaag caaatcttgc agttgaagta
     7681 agtgttcaaa aagcctggca agaacgtatg gttagagcct ggcaagcaag tggcagtaag
     7741 ttagtgtccg aaaaagtgaa gcaggcggtg gaaaattggt gaacccatcg actcagcttg
     7801 cgcttgaaat ttcaaaatca attctggctt gtggcgtaac tgatgtagtg gtttgcccag
     7861 gctcaagatc agcaccgcta gcttggcaga tggcacaact ggcaagtaaa caaaaaatca
     7921 aactacacac tcgaatcgat gaacgcgaag ctggtttttt tgcacttggg attgccaagg
     7981 cgacaaaacg cccagttcca gtaattgtta cctcaggaac tgccgtcgca aatttattgc
     8041 cagccgttgt cgaggcgtat cacagcggaa tcccactagt ggttctgagc gcagatcgac
     8101 cagcggcggt gcgcggaaat agtgcaccac aaactacgaa tcaagttgga atatttagca
     8161 gcttcgtaaa ggcttttgcc gatactgcgg tgccagatcc agaaatactt gcagcgatca
     8221 aaagctcact tcaggttcat cctggtccgg tgcaaataaa tgcgcagttc gatatgccgc
     8281 taatgcctga agatcctgaa atagattttg gccaaatcag cgaaattgaa tttatcaagg
     8341 aagttcaaca aaaagagaag ttggaattac caagtaaagg tttattagta attggagatc
     8401 ttccagatgg tcaacatgtc caagagttaa ttgatctggc ggaagattgt ggctatccaa
     8461 taatttggga accaacggct caagtaatgc gaagtaaaaa tgcgatcgat catggcgcat
     8521 tactacttca aagcgataaa ttgcctaaac cagaagtagt gattacggct gggttaattg
     8581 gattatctag atcagttttg aatgtgttga aaacgacacc acaccacatc gcaatacatt
     8641 tatcggccac cggagcacaa ttacccaatc cggtgcttag cgccaaacaa actttgaccg
     8701 ctgttcctga aataaaaaca ttagtagaca aagattggct ggctaagtgg cagcaagcgg
     8761 cgaaccatgc cagtgatgta ataagtaaaa aaataaaatt agaaactttg accggaccca
     8821 gtgctgccgt aacactttgg aatcacgttc cagcagatag cacactattt atttcttcat
     8881 cttggtcagc acgtcatatc gagatgtatg cacaagctcg gccagattta aaaacatttg
     8941 gtaatcgtgg cgttaatgga attgatggct taatttcgac tgcagctggc gtcgcactca
     9001 cgcaacaaaa ccgaacctat ctgctaatcg gtgacattgc gtttttgcat ggaatgggtg
     9061 gactaaatat ttctgcagaa aatgaaattc ctaatctgac tgttgtggtt ttagacaatg
     9121 atggaagtgg aatctttagt cagcttgaac aaggcgcggc aaagtatcaa aaacattttg
     9181 aacaaatctt tggtacaccc cacggcaaag acctatgggt tattgcagaa agtctcggtg
     9241 tcccagcaac cagagtgaca actaaatcgg aattactaaa agctcttgct ctttcagaca
     9301 aaatacctgg actacaagta attgtctgca gtacaggtag ccgcaaagac gagcaagagt
     9361 taattaacct aattagtgcg caggtttctg actcaattta aatccagaat atgtcagtta
     9421 aatcaactaa tgttgcatta atcaaaattc tggagacgta actaaagtgg ccgataagtt
     9481 tttaaaagtt gaagttgaac agccagaaaa tgcaaagttg cctaaacgcg tttcattttc
     9541 gcaaatgtca ctgtttcaac aatgtgggtt aaagtacttt ttttcttata tcgataattg
     9601 gcgcgagccg ccaactgcgg ccttggctgg cggatcgata actcatgacg tcatcgagcg
     9661 cctttatcgg ttacctaatg aagaaagaac tctcgccaaa gccgtcgaaa ttttgcgaga
     9721 gtacgggcca aaatttttag ctcagccgga atatcaaaaa ttcgctgaag atttaaacat
     9781 gaagcattcg gttcgcgatg cagtcgaaaa cctattcgca gtcgaagatc caaaaagttt
     9841 ggttgttcaa ccagagcatt tggaaatgga acttaatgtt gatatcaacg gagttaattt
     9901 ttttggaaaa gttgatcggt ttacccagga tggcgccaat cgggtaaccg actataaaac
     9961 tggcaaaagc ccgggaaaat ttgttgacgg aaagttggcg caaccgtatc tatacgctct
    10021 tggctacaaa cttcaacatg atatcgatgt tgatgaagtt gaactaattt atttaaatgc
    10081 caaagaagtt attcgccgac cagctgaatg gggacaaata accaaggttg gcgaagattt
    10141 agcaacaatg cgaaatcaaa gccaagcaag ccttaacgag tctgcatggg atgcaaaagt
    10201 ttcgaagtta tgcgattact gctcttttca agaagtttgt aaagcgcgca atccagatgc
    10261 accgcttcca ggaagtatta aaagtgatga aaagctttac agtattggtt tgattcaaaa
    10321 atagctttta atttgtcagt ttagtcaagc catctttaag cacagttaaa aatagatcta
    10381 catcttcttt ggtaataact agtggtggct taatttttaa tacattgtta gcaggaccgt
    10441 cactagataa aaagacacct tgaatttttg catactcgat caagtcagca gttaattgcg
    10501 ttgctggaac ttgagtttct tgatctaata cgaattcaac tccaatgaat aatccacttc
    10561 cacgtacatc cccaataatt gggattgact tacctaattg gcgcacacta tccatcaagt
    10621 agttacctaa atgatttgca ttcgcttgta acgcctgatc tgcaactaca tctagaactg
    10681 cttgaccaat agccgcagaa actggattgc caccaaaagt attgaaatac tccatgccag
    10741 ttaagaatga atgcgcgatt tcaggagtgg taaccactgc ggctaacgga tgtccattac
    10801 cgattggctt tccaatagtg acaatgtcag gaacgacatt gtgtaattca aatccccaga
    10861 agcactcacc aacacgaccc attccaatct ggacctcgtc tgcgatacaa actccacctg
    10921 ctgcccgagt ttgttcgtaa gccaacggta aaaatccggg tgccaaagtt atttgccccg
    10981 cagtgctgat tattgattcg ctgatgaagg cgcacagggg ttgattcaag tcggtcaatt
    11041 gagcggcaaa ttcggttgcg tatttttcgc cgctacctgc tccgcgatga agtcctttat
    11101 aactatctgg tgttgctacc actcgaacat gatctggtct acccaggcca ccttttccag
    11161 aaaatttata tgggctgatg tcgataatgc tggtgacgtg accgtggtat gcctgatcta
    11221 aaacgatcat gccttttgat tttgtgtgtg cttgcgctaa gcgaagggct agatcatttg
    11281 cttctgatcc gctatttaca aagaagacaa catttaatgg atcaggcatc gtgctggcta
    11341 aagctctggc gtactcaatc gcattctggt ttaagtaccg agtatttgtg tttaacgtag
    11401 ccatttgctt acttccagct tcgaccactc gcggatgaca atgtccaaca tgtgcgacgt
    11461 tattaactaa atctaaatat ggttttccag cagtgtcata aagataagca ccctggcctg
    11521 aaacaatttc aatcggttct ttgaaattca aactaagtgc ccgtgacata actacttgtc
    11581 gctctttagc gagtacatct ttgggtaact tagtgtgcgc atcagtgc
//
LOCUS       DBNHVN010000095         2499 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_982368, whole genome shotgun sequence.
ACCESSION   DBNHVN010000095 DBNHVN010000000
VERSION     DBNHVN010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_982368"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1832)
                     /locus_tag="AC16Y1_01805"
     CDS             complement(141..1832)
                     /locus_tag="AC16Y1_01805"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HIR5831231.1"
                     /translation="MTPEELKAAQEDIAVHGARAGDFVRLGDTDLIIRVEGDDRQFGS
                     ELQMGFGKSARDGMGLKSVNSSQSCDLVITNALLIDAVSGIKVTSIGIRNGRISAIGR
                     PGNPDTMDGIDVVVGSGTVVIPGEGFIVTAGGIDTHVHALSPRVCDSALSSGVTTIIA
                     QEFGPFWGVGVSSAWALAHGHEMFSAWPLNVGILGRASASTTAPLEEALSGGICGFKI
                     HEDTGAHPRTIDTALTFAEEHDLAVALHTDGLNEMLDVNGTVSAIAGRAVHAFHVEGC
                     GGGHAPNVLKLAGRENILASSTNPTLAYGINAADEHVAMIISAHGMNSDLPSDVALAK
                     QRVRNATMAAENRLHDMGVIPVTSSDALGMGRADDTWRKTFAMASMFADQAVLDGSVA
                     PNEVVTANERVLRHVAKITINPALVHGLSHEVGSVQVGLVADLVLWRYADFASKPDLV
                     IKSGIPAWGVVGDPGATVKEAQPLLIGPQFGAYGATAADLGIHFTNQAVPNLPGRRRS
                     VAVKNTRTIRGSQMVRHGVMGEIEVSANGEVVTFNGEPVSSSPVTNASLTRTYLL"
     gene            complement(1829..>2499)
                     /gene="ureA"
                     /locus_tag="AC16Y1_01810"
     CDS             complement(1829..>2499)
                     /gene="ureA"
                     /locus_tag="AC16Y1_01810"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016640472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HIR5831232.1"
                     /translation="TPTEQDRLLLFTAGQLAKTRLARGLKLNKPEAVAVICNAVIEAA
                     RDGATHSEALSAGQNALSPADLLPGVSALLSGISVEAVFTDGRRLVVVNFENEESNFP
                     GKVTRLAPFEAPMVTPTSIAVKNESAVPVAVTSHMHFMEVNPKLRFQRETAYGMRLNI
                     PSGTHVDFPPNEIVHVELIPIEGARVVIGFAGIIDGDLTNPNGLEIAMQRLTEFGYLT
                     GEPV"
BASE COUNT          680 a          630 c          517 g          672 t
ORIGIN      
        1 caatttgata tgcagtggct aaaccaatta cgccaccacc caagacagtt acatttggtt
       61 ggctcataac taaagattag agcccaacgg tttaaaaaat aactcggccg ccaaaatatg
      121 gcaacaagga aaaaccatgc ttaaagtaaa taggtgcgag tcagggacgc gttggttact
      181 gggctacttg aaaccggttc accattaaag gtgactactt caccgttagc gctaacttca
      241 atttcaccca tcaccccatg acgaaccatc tggcttccgc gaatagtgcg cgtatttttc
      301 actgccacac tgcggcgcct acctggcaaa tttggcacgg cttgatttgt gaaatgaatt
      361 cctaggtctg ccgcagtagc tccatatgca ccaaattgtg gaccaattaa aagtggttga
      421 gcttccttga cggttgcacc aggatcgcca acaacccccc atgctggaat tccagattta
      481 attactaaat cgggtttact cgcgaaatct gcatagcgcc ataagactaa gtctgcgact
      541 aagccaacct gaactgaacc gacttcgtgt gacaagccat gaaccaatgc tgggttaatt
      601 gttatttttg cgacatgcct aaggacacgt tcgttagcag ttacaacttc attaggtgca
      661 acactgccat ccagtactgc ttgatcagcg aacatactgg ccatcgcaaa agttttccgc
      721 caagtatcgt ccgcgcggcc catgccaagg gcatcagatg acgtaactgg aattacgccc
      781 atatcgtgca atctattttc ggcagccatg gttgcattcc ggacccgctg ttttgctaaa
      841 gccacatcgc taggaagatc tgagttcatt ccatgtgcgg aaataatcat cgccacatgt
      901 tcatcagcag catttattcc gtaagccaat gttgggttgg ttgacgaagc caatatattt
      961 tcacgtccag ctagttttaa tacgttaggt gcgtggccac cgccgcaacc ttcaacatgg
     1021 aaggcatgaa ctgctcgccc ggcaatcgca ctcacagttc cattcacatc gagcatttca
     1081 tttaagccat ctgtgtgtag cgcaaccgct aagtcgtgtt cttctgcaaa agttaacgca
     1141 gtatcaatgg tgcgtggatg tgcaccagta tcttcatgga ttttgaaacc acaaataccg
     1201 ccacttagtg cctcttctag tggtgcagtt gtgcttgccg aagctctacc taagattcca
     1261 acattcaacg gccaagcgct aaacatttca tgaccatggg ccagtgccca ggcggaacta
     1321 acgccaacac cccagaatgg gccaaactct tgcgcgatta ttgtggttac accgcttgaa
     1381 agtgctgaat cacatactct tggcgataaa gcatgaacat gtgtgtcgat gccacccgct
     1441 gtaacaataa atccttcacc gggaattact actgttccag agccaacaac gacatcaata
     1501 ccatccatcg tgtctggatt tccaggtcgg ccaattgcac taattcgacc attgcggatt
     1561 ccaattgaag ttactttgat tccagaaacg gcatctatta acagtgcatt tgtaattact
     1621 aagtcgcaag attgagaact attaactgat ttaaggccca ttccatctct ggcactttta
     1681 ccaaaaccca tttgcaattc actaccgaat tgacgatcgt caccttcgac tcgaattatt
     1741 aagtcagtat caccgagtcg aacgaaatct ccggctcgtg caccatgcac cgcgatatct
     1801 tcttgtgctg cttttaactc ttctggagtc atactggttc tccagtcaag taaccaaatt
     1861 ctgtaagacg ttgcattgca atttctaggc catttggatt agtcaaatcg ccatcaatga
     1921 ttccagcaaa tccaataact acgcgtgctc cttcaattgg aattaattca acgtgcacta
     1981 tttcatttgg tggaaaatcc acatgtgttc ctgatggaat gttcaggcgc atgccgtaag
     2041 cggtttcgcg ttgaaacctt agctttggat tcacttccat aaaatgcatg tgcgaagtta
     2101 ccgcaactgg aacagctgat tcatttttaa ctgcaatcga agttggagta accataggcg
     2161 cttcaaacgg tgccaagcgg gtaactttgc ctgggaaatt actttcctca ttttcaaaat
     2221 tgactactac taaacgacgt ccgtcagtaa aaactgcctc aacggaaata ccagaaagta
     2281 gtgcgcttac tcctggcaat agatccgcgg gactcaatgc attttgacca gcagaaagtg
     2341 cctcgctatg tgttgcacca tctcgagcag cttcgattac tgcattacaa atgacagcga
     2401 ctgcttctgg tttatttagt tttagtcctc gggccaaccg agtttttgcc agttgcccag
     2461 cagtgaatag caaaagtcga tcttgctcag ttggcgtaa
//
LOCUS       DBNHVN010000096         1141 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_908412, whole genome shotgun sequence.
ACCESSION   DBNHVN010000096 DBNHVN010000000
VERSION     DBNHVN010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_908412"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="AC16Y1_01815"
     CDS             <1..285
                     /locus_tag="AC16Y1_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017488323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR5831233.1"
                     /translation="ELPHSIAVVIEEMGLRKDRSPENPLIDIYAIIFVERDSQKHIVI
                     GKNGSRLKEVGTTARLAISKMLGTPVFLDLRVKVAKDWQRDPKQLNRLGF"
     gene            complement(282..>1141)
                     /locus_tag="AC16Y1_01820"
     CDS             complement(282..>1141)
                     /locus_tag="AC16Y1_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR5831234.1"
                     /translation="VLLGLIIFIIGFLALFGGVLFTQAASLVGAIPNLLSNVVTWLNS
                     TFELTLNSSEIQDSLNLRPDQVALWASNFAGGVVGLVSTIFGAIFQLMTLLLFAFYFA
                     ADAERVRQTVGRWLSPTAQEIFVTTWDIAVQKTGGFVASKLFLASLSAAAHGIFFAAI
                     GVPYWLPMAIITGITSQFIPVIGTYLGILIPTLFTLFNDPLNALWIIVFASIYQQFEN
                     YILTPKISRLMMNIHPAIAFASVIVFANLFGAAGALIAIPIAAAIVAVSDTYGKRYEL
                     IPKLNGKTN"
BASE COUNT          388 a          219 c          246 g          288 t
ORIGIN      
        1 gagttgccgc attcgattgc ggttgtaatc gaagaaatgg gtttacgtaa ggatcgaagt
       61 cctgaaaatc cattgattga tatttacgcg attatttttg tcgaacgcga tagtcaaaaa
      121 cacattgtga ttggcaaaaa tggcagcaga ctaaaagaag ttggcacaac ggccaggctg
      181 gcaatctcaa agatgcttgg tacaccagta tttttagacc tgcgggtcaa agtggcgaag
      241 gattggcaac gagatcctaa gcagttaaat cgacttggat tttagttagt ctttccattt
      301 agttttggaa ttagttcgta gcgttttcca taagtatctg aaactgcaac gatggcagcg
      361 gcaatcggaa ttgcgattag tgcaccagcg gcgccaaata agttagcaaa cacaatgacc
      421 gaagcaaaag cgattgctgg atggatgttc atcatcaatc ggctaatttt tggcgtcaaa
      481 atataatttt cgaattgctg gtagatagaa gcaaaaacaa taatccataa tgcgtttagt
      541 gggtcattga aaagcgtgaa caaagttgga ataagtatgc ctaaataagt tccaataact
      601 ggaataaatt ggctcgttat gccagtgata attgccattg gtaaccaata gggcacacca
      661 atagcagcaa agaaaattcc atgagcggcc gccgacaaac ttgctaaaaa taatttagag
      721 gcaacaaaac ctcctgtttt ttgaacagcg atgtcccaag tggtaacaaa aatttcttga
      781 gcagtcggac tcaaccaacg tccaactgtt tggcgaactc gctctgcatc tgccgcaaaa
      841 taaaaggcga ataacaaaag cgtcattaat tgaaaaattg caccaaaaat tgtggaaact
      901 aaaccaacga cgccaccagc aaaattactc gcccaaagtg caacttgatc tggccgtaag
      961 tttaatgagt cttgtatttc tgaactattt agcgttagtt caaaagttga atttagccaa
     1021 gtcaccacgt tacttagtaa attcggaatt gcaccgacta gtgaggctgc ttgggtaaat
     1081 aaaacgccac caaaaagtgc cagaaagccg atgatgaaaa taatcaagcc aagcaaaaca
     1141 a
//
LOCUS       DBNHVN010000097         2457 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1800255, whole genome shotgun sequence.
ACCESSION   DBNHVN010000097 DBNHVN010000000
VERSION     DBNHVN010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1800255"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1045)
                     /gene="dapC"
                     /locus_tag="AC16Y1_01825"
     CDS             complement(<1..1045)
                     /gene="dapC"
                     /locus_tag="AC16Y1_01825"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR03539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HIR5831235.1"
                     /translation="MFTPLASGLPDFPWDRIAAAGDKARSHKNGIIDLSIGTPVDDVP
                     QVIQDALANHANAPGYPTAAGIEPLRQAWVDWVKSVTDCQLTVNQVAPTLGSKELVAW
                     LPIIFGLKKGSTVAIPELAYPTYAVGAMMAHADVQTYRTAAEIGDSVDLIWVNTPSNP
                     TGQVLSVIELRDLVLRGREIGAPIVSDECYIELGWSTTPVSILDRRVNYGDLTGILAV
                     HSLSKRSNLAGYRSGSVSGDQELINNITGLRKHAGMLMPTPVQHATIAGLGDTKHVAS
                     QKAIYGARRDVLHTALVNAGFEISHSEAGLYLWATRGEDCFATVDWLATRGILVAPGD
                     FYGAAGAKHVRVAF"
     gene            complement(1048..1368)
                     /gene="fdxA"
                     /locus_tag="AC16Y1_01830"
     CDS             complement(1048..1368)
                     /gene="fdxA"
                     /locus_tag="AC16Y1_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIR5831236.1"
                     /translation="MTYVIALPCVDLKDKACVEECPVDCIYEGDRMLYIHPDECVDCG
                     ACEPVCPSEAIFYEDDLPEEHAQFAQINKDFFATLGSPGGAAKVGKTSNDPEFVKSLP
                     PQEH"
     gene            complement(1457..1810)
                     /locus_tag="AC16Y1_01835"
     CDS             complement(1457..1810)
                     /locus_tag="AC16Y1_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831237.1"
                     /translation="MLKVRNWLPALIVGLLSSSLAAFVHADRIKISDQSIYFGLVLAP
                     LFLLATQRWLMQAYRNRISGIAFFVAWLLVSVRLSIPNSGGDLALASSWFSTAYLGVT
                     ALVLSMSMVLSPKVK"
     gene            complement(1810..>2457)
                     /gene="mshB"
                     /locus_tag="AC16Y1_01840"
     CDS             complement(1810..>2457)
                     /gene="mshB"
                     /locus_tag="AC16Y1_01840"
                     /EC_number="3.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035595 - N-acetylglucosaminylinositol
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-
                     D-glucopyranoside deacetylase"
                     /protein_id="HIR5831238.1"
                     /translation="VTDWRFLGGPGTYRDSGMIGTAPNERKDNFWQADLLDATKHLVK
                     IIREVKPQVLITYDDFGGYGHPDHIQAHRVATYAVTLAAVPSFGLDLGDPWEVQKVYW
                     TAFPKSVIKAGIEELKAKGDTSDFATQDPDDLPFACDDSLVTSSIDAGEFAEVKLAAL
                     AAHETQISTDDSFFALSNNLGSRVMGVEYFRIAKGNVSGPFNEQGHEIDLFSGVI"
BASE COUNT          712 a          601 c          498 g          646 t
ORIGIN      
        1 tgaatgccac acgaacatgc ttagccccag cggctccgta aaagtcacca ggtgcaacca
       61 atattcctcg agtagctaac caatcaactg tggcgaagca atcttctcct cgagttgccc
      121 acaaatacag gccagcttca gagtgactaa tttcaaatcc agcatttacc agcgcggtgt
      181 gaagtacatc acgtcttgcg ccataaatcg ctttttggct cgccacatgt ttggtgtcac
      241 ctaagccagc gatcgtggca tgttgcaccg gagttggcat caacatacct gcgtgttttc
      301 gcaacccagt tatgttgtta attaattctt gatcaccaga aaccgaacct gatctatagc
      361 cagctaaatt cgatcgcttt gacagcgaat gtactgccag gattccggtt agatcgccat
      421 aattgactcg tcgatccaaa attgaaactg gtgtagtcga ccaacctaat tcgatataac
      481 actcatccga aacaatcggt gcaccaattt ctctacctcg taaaactaaa tcgcgaagtt
      541 caatgactga taaaacttgg ccggttgggt tacttggcgt attaacccaa attaaatcaa
      601 cactgtctcc aatttcagca gcagttcgat aagtctgtac atctgcatgc gccatcatgg
      661 ctccaactgc ataagttgga tatgccagtt ctgggattgc aactgtgctg ccttttttca
      721 gaccaaaaat aatcggcaac caagcgacca actcttttga acccagagtg ggcgcaactt
      781 gattaactgt taattggcaa tcagtaactg atttaaccca atcaacccat gcttgccgaa
      841 gtggttcaat tccagcagca gttgggtaac caggtgcgtt tgcatggtta gctaatgcat
      901 cttgaattac ttggggtaca tcatcaaccg gtgtaccaat agagaggtca atgattccgt
      961 ttttatgtga acgcgctttg tcaccagcag cagcaattcg atcccaggga aaatcaggaa
     1021 gacccgaggc aagcggagta aacagtttta gtgctcctgc ggaggcaacg actttacaaa
     1081 ctcaggatcg ttgcttgtct tacccacttt tgccgcgcca cctggtgagc caagtgtggc
     1141 aaagaaatct ttattgattt gagcaaactg agcgtgctct tctggtaaat catcttcgta
     1201 aaaaattgct tcggatgggc aaacaggttc gcaagctcca cagtcaacac attcatctgg
     1261 atgaatgtaa agcatgcggt caccttcata aatacagtcg actgggcatt cttcaacaca
     1321 tgccttgtct ttaaggtcta cgcatggaag tgcgattacg taagtcacta ttcctccaac
     1381 aattcttttg gtaactcaac gctactctgc tttttgcatt tttgcccact agggctactt
     1441 aatattgcga acagccttat ttaaccttag gagacaaaac catactcatt gaaagtacta
     1501 aggcagttac cccgaggtaa gccgtggaaa accagctaga tgccaaagcc aaatctccac
     1561 ctgaattcgg gatcgaaagg cgcactgaaa caagtaacca ggcgacgaaa aatgcaatcc
     1621 ctgaaattcg atttcgataa gcctgcatta gccatctttg cgtagccaat aaaaaaagtg
     1681 gtgccaaaac caaaccaaaa taaatggatt gatctgaaat tttaattcga tcggcatgta
     1741 caaaagcagc taatgaactc gaaagtaaac caacgataag cgccggaagc caattacgaa
     1801 ccttaagcac tagataacac cactgaataa atctatttcg tgtccttgtt cattaaacgg
     1861 gccgctcaca ttacctttcg caattcggaa atattcaacg cccatcactc ggctacctaa
     1921 attattggat agcgcgaaaa acgaatcatc agtgctgatc tgagtttcat gtgcggccag
     1981 tgcagcaagt tttacctcgg caaattcgcc tgcatcgata cttgaagtaa ctaatgaatc
     2041 gtcacaggca aacggcaaat cgtctggatc ttgtgttgcg aaatctgaag tatcaccttt
     2101 ggctttgagt tcctcaatac cggctttaat cacactctta ggaaatgccg tccagtaaac
     2161 tttttgtact tcccacgggt cacccagatc taagccgaaa cttggtaccg cagctaacgt
     2221 aactgcataa gttgcgactc gatgtgcctg aatgtgatct ggatgtccat atccgccaaa
     2281 gtcgtcgtaa gttatcaaca cctgtggttt aacttcgcga ataattttca ctaaatgttt
     2341 ggtggcatca agtaagtcag cctgccaaaa attatcttta cgttcatttg gcgcagtacc
     2401 gatcattcca ctgtcgcgat acgtgcccgg accgcctaaa aaacgccaat cagtgac
//
LOCUS       DBNHVN010000098         4015 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_537022, whole genome shotgun sequence.
ACCESSION   DBNHVN010000098 DBNHVN010000000
VERSION     DBNHVN010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_537022"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..>4015
                     /locus_tag="AC16Y1_01845"
     CDS             26..>4015
                     /locus_tag="AC16Y1_01845"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016944.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - L-glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HIR5831239.1"
                     /translation="MDLVGRNPNDLAAMIKGHFELGQSRQLGSAVVQTLAPSLETSGW
                     SSSHTIIQLVTDDKPFLVDSVAAELNMQKRGVHLIMHPRFLVHRDKKGRMVEILSSDY
                     ADQAHLPSDAVVESWICVEIDRETNQEELVQITNRIKQVLDDVKIAVADWASMRSATQ
                     NVISVIENAQYFSAEDKKNAAQFMRWLDNDHFTYLGYSYNKVSNGVVSTVTGSSLGVA
                     RAEKESSASANYLIQGSVYSPIILVTKTSNKATVHRPIYMDYIGVREVNETGQLVGEH
                     RFIGLFTSTAYTESATSIPLLAKKVNNVISAMGLGINTHSGKDLMQFIATYPRDELFQ
                     ISSVELEEVARGVLQIQERKQVRLFARTELFGRFVSALVYFPRDRYTTEVRLRMEEVL
                     KTAFGADSIDHSARVSESVLARLHFVIRMPRGKSVPTVDLDQLEQKLAEATRFWDDDF
                     AEALVDQVGEEEAVRLLRTWSESFPESFKEDVPAPSAVNHLKVLEEVEALGGGQIKVA
                     MYQPVLEGDGTRRFTIYRVGDPITLSAVLPMLHDLGIEVIDERAYDLRRTGQEVAWVY
                     DFGIGFDESLTPELDTLSDRFCATFMATWSGEVDSDGFNALVTHGGLAVRHVAVIRAY
                     AAYLRQVGIPFSQGYLQQVLISNVGIVKLLLELFKVRFDPRLEIDRAAAETDLVKQIN
                     KALDAVASLDHDRIMRTSIALVRATLRTNVYQKDFSGNYLAVMAFKLDPTKIPDLPLP
                     VPKFEVWVYSPRVEGIHLRFASVARGGLRWSDRQEDFRTEILGLVKAQMVKNTVIVPS
                     GAKGGFVVKRGPNPADREAWLAEGISCYQSFIGALLDVTDNLVDGNVVPPTDVVRHDV
                     DDPYLVVAADKGTATFSDIANQISLDRSFWMGDAFASGGSVGYDHKGMGITARGAWES
                     VKRHFLELGIDTQTQDFTVVGVGDMSGDVFGNGMLLSQHIRLVAAFDHRHIFLDPHPD
                     ATNSWVERKRLFDSPRSSWEDYNQSVISFGGGVYQRSAKSIDLSHEVKEVLGIDQSID
                     SMSPNQLLSAILRAPVDLVWNGGIGTYIKAATETHAQVGDKANDGLRINGAELRCKVV
                     GEGGNLGCTQLGRIEAARAGVKINSDAIDNSAGVDTSDHEVNIKILLNLAMETGKLSI
                     DQRNTLLSSMTDEVGLLVLQDNYQQNVILEQSRFKAVEMLRVHQRLMQNLESIGLLNR
                     TVEYLPTDSQIDTLHMQKLGLSSPELAVLMAYVKIDLTSAFTEDSVVDEDWCIPVLTN
                     YFPSALRDRYSDLMLRHPLKREIIATVMTNDMVNRGGISYALRANEESGATPADILRA
                     YVVSKEVFGF"
BASE COUNT         1084 a          824 c          939 g         1168 t
ORIGIN      
        1 tgattatttt gattgagttg caccgataga tttagttggc cgcaatccaa acgatttggc
       61 cgcaatgatt aaaggtcact ttgaattggg tcaatctcgc caattgggta gcgccgtagt
      121 ccagacactg gcgccatcat tagagacttc tggttggtca tctagccaca caatcattca
      181 acttgttact gacgataaac ctttcttagt tgattcggtg gccgctgaat taaatatgca
      241 aaagcgtggt gttcatctga ttatgcatcc acgcttttta gttcatcgag acaaaaaagg
      301 ccgaatggtc gaaatacttt cttctgatta cgcagatcaa gctcacttac caagtgatgc
      361 ggtggttgaa tcttggatct gcgttgaaat cgatcgtgaa acaaatcaag aagaactagt
      421 acaaataact aatcgaatta aacaggtatt agacgatgta aaaattgccg tagccgactg
      481 ggcgagtatg cgatcggcaa ctcaaaatgt aatctcggta atcgaaaatg cccaatattt
      541 ttcagctgaa gataagaaga atgctgcgca atttatgcgc tggctagata atgatcactt
      601 tacgtacttg gggtattcgt ataacaaagt tagtaacgga gttgtcagta ctgttacagg
      661 atcaagcctc ggtgtggccc gcgctgaaaa agaatcttca gcttctgcca actacttaat
      721 acaaggttca gtttactcac ctattatttt ggtgacaaaa acgagtaata aagcgactgt
      781 gcatcgacct atttatatgg actacatcgg tgttcgggaa gttaatgaaa ctgggcaatt
      841 agtcggggag catcgcttta ttggtctgtt tacttctact gcctataccg agtcagctac
      901 gtcgattcca ctattagcca aaaaagttaa taacgtaatt tccgccatgg ggcttggcat
      961 taacacccac agtggaaaag atttaatgca atttattgcc acttatcctc gtgacgaact
     1021 atttcaaatt agctctgttg aacttgaaga agttgccaga ggtgtgctgc aaatccaaga
     1081 gcgtaaacaa gtccgacttt ttgctcgcac tgagttattt gggcgatttg tctcggctct
     1141 ggtgtatttc cctcgagatc gttacaccac cgaggttcga cttcgaatgg aagaagtttt
     1201 aaaaacagct tttggcgcag attcaattga ccattccgca agagtttctg agtcagtatt
     1261 ggcaagactc cacttcgtaa ttcgaatgcc gcgaggcaag agtgtgccaa cggttgatct
     1321 tgatcaactt gagcaaaaac tcgcagaagc cactcgattt tgggatgacg attttgcaga
     1381 ggcgttagtt gatcaagttg gcgaagagga agctgtgcgc ttacttcgca cttggtcaga
     1441 atcttttcca gagtcattca aggaagatgt accggcgcca agcgctgtaa atcacctaaa
     1501 agtactcgaa gaagttgagg cactgggcgg cggccaaata aaagttgcca tgtaccagcc
     1561 tgtgctcgaa ggagacggca ctaggcgttt cactatttac cgcgtgggcg atccaattac
     1621 actttctgcc gttttaccaa tgttgcatga cctaggtatt gaagtaattg acgaacgtgc
     1681 ttatgacttg cgtcgcacag gccaagaagt tgcatgggtt tatgactttg gtatcggctt
     1741 tgatgaatca ctaactcctg aattagacac cttgtcggat cgtttttgtg cgactttcat
     1801 ggcgacttgg agtggcgagg tagatagtga cggctttaat gctctagtga cccatggtgg
     1861 tttagcagtt cgccatgttg ccgtaattag agcttatgcg gcctatttgc gccaagtggg
     1921 tattccgttt agtcagggtt atttacaaca agtattgatt tcaaatgttg gaattgtgaa
     1981 gttattactt gagttattca aagttcgatt cgatccaaga ttagaaatcg atcgagctgc
     2041 agccgaaact gatttagtca aacaaattaa taaagcatta gatgcagtag ctagccttga
     2101 tcatgatcga attatgcgca cttccatcgc cttagttcga gcaactttgc gcacaaatgt
     2161 ttatcaaaaa gatttttctg gaaattactt agctgttatg gctttcaaat tagatccaac
     2221 caaaattcca gacctacctt taccggtgcc taagtttgag gtctgggttt attcaccacg
     2281 cgttgaaggc attcacttaa ggtttgcttc tgtggcgcgc ggcggattgc gctggtcaga
     2341 tcgccaagaa gacttccgca ccgaaatcct tggcttggtt aaagcgcaaa tggttaagaa
     2401 cacggtcatc gttccttcgg gagcaaaagg cggttttgta gttaagcgcg gaccaaatcc
     2461 tgctgatcgc gaagcatggc tagctgaagg aatttcttgt taccagtcgt ttattggcgc
     2521 cttgctagat gtgactgaca acttggttga cggcaatgtt gtgcccccga cggatgtggt
     2581 tcgacatgat gttgacgacc cttatttagt tgtggctgct gataaaggca cagcaacttt
     2641 ttctgacatc gcaaatcaaa tttcactgga tcgcagtttc tggatgggtg atgctttcgc
     2701 ctctggtggt tccgtcggtt atgaccacaa gggcatgggt attactgctc gcggtgcatg
     2761 ggaatcagtt aaacgccatt tccttgaatt aggtatcgat acacaaactc aagactttac
     2821 tgttgttggc gtaggcgaca tgagtggtga cgtttttggt aatggcatgt tgctgtccca
     2881 acatattcgc cttgttgccg cttttgatca tcgccatatt ttccttgacc cacaccctga
     2941 tgcaactaat tcatgggttg aacgtaaacg gttatttgat tcaccaagat catcttggga
     3001 ggactacaac caaagtgtta ttagttttgg cggcggtgtt taccaacgtt cggctaaatc
     3061 cattgacctt tcgcacgaag ttaaagaagt tttaggaatt gatcaatcaa tcgactcgat
     3121 gtcaccaaat caattacttt ctgcaatttt gagggcgcca gttgatctag tttggaatgg
     3181 cggcatcggt acttacatca aagctgccac tgaaacccat gctcaagttg gcgataaagc
     3241 caatgacgga cttcggatta atggcgcaga attgcgttgc aaagtagttg gtgaaggtgg
     3301 caacttaggc tgcacacaat taggtcgaat cgaagcagct cgagctggcg taaaaataaa
     3361 ttctgatgca attgataact cggctggtgt tgatacttct gaccatgagg tgaatatcaa
     3421 aattttgctt aacctggcaa tggaaactgg caaattaagt attgaccagc gcaacacctt
     3481 gttaagcagt atgactgacg aagttggcct actcgtttta caagacaact atcaacagaa
     3541 tgtgattttg gagcagtcca ggttcaaggc tgttgagatg ttgcgcgtcc atcaacgatt
     3601 aatgcagaat cttgaatcca ttgggttatt gaatcgaaca gttgaatatt taccgactga
     3661 ttcacaaatt gataccttgc atatgcaaaa acttggctta tcctcacctg aacttgccgt
     3721 gttaatggcc tacgtgaaga tcgatttaac ttcagcgttt actgaggatt cagttgttga
     3781 cgaagactgg tgtattccag tgctcactaa ttactttcca tcagcacttc gggatcgata
     3841 ttcagatttg atgcttaggc atccacttaa aagagaaatc atcgctacgg tcatgacaaa
     3901 tgacatggtt aaccgaggtg gcatttccta cgctttgcgg gcaaatgaag aatctggcgc
     3961 aacgcccgct gacatcttgc gagcctatgt cgtttcaaaa gaagtatttg gattc
//
LOCUS       DBNHVN010000099         3064 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1355326, whole genome shotgun sequence.
ACCESSION   DBNHVN010000099 DBNHVN010000000
VERSION     DBNHVN010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1355326"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..>3064
                     /gene="mfd"
                     /locus_tag="AC16Y1_01850"
     CDS             124..>3064
                     /gene="mfd"
                     /locus_tag="AC16Y1_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013003042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR5831240.1"
                     /translation="MSLNLITTALLADEKVQTALELIKKKQSVEFGIPQTAMPYLAGL
                     IAKNSTGSLIICATDRIAEDVAAQLAEYVPANQVAFFPAWETLPHERLSPSGDTIGTR
                     MAILRKLANPQEAGEINYLVTSVRAVIQPMASGLGNIAPLVINEGQTMAQEKVIEQLS
                     YLGYERVELVDRRGQFAVRGGIIDVFPPSQPHPVRLEYWGDDVDDIRMFSVADQRSLD
                     LEIDQLFATPCRELLITDSVKKKAQKFAIEHPEFAEVFEKVAAGIATEGLESFIPILA
                     PALETLFDLVPQTWTIQELEPERIATRAQELIDTSAEFLAASWGNTTAGNAIPVDLGR
                     GFYGYRELKSEYLNRVWISQSALMGPETITPDLEPTPDYRGQFDSLVTQINSWWQQEF
                     RVVISMAAHGTAERLVNSLGEFNIAARLATEPEPIVKRVEVITGHQENGLVFNAGKLV
                     FLTETDLFGNRSTTKSARAMPSRRRTAIDPLTLKTGDFVVHEQHGVGKYIEVAQRVVQ
                     GATREYLVIEYASSKRGQRGDRLYVPTDQLDLVTKYVGGELPTLNRLGGGDWAQTKSR
                     ARRAVRQIAGELIRLYAVRMASKGHAFAPDSPWQQELEDAFEYVETPDQLSTINEVKA
                     DMERDVPMDRLVCGDVGYGKTEIAVRAAFKAVQDGKQVAVLVPTTLLVQQHMETFARR
                     YAQFPVKLAALSRFQTDAEVKKTFAGIQDGTIDIVIGTHRLFNPEVRFKDLGLVIVDE
                     EQRFGVEHKEHLKALRANVDMLTMSATPIPRTLEMAVTGLREMSTIQTPPEERHPVLT
                     SVGPYDEKYIVAAIHRELIRDGQVFFVHNRVESINRVASRLSELIPEARIAVAHGQMN
                     EKQLEQIIVDFWQHKFDVLVSTTIIESGLDIPNANTLIVDRADTFGLSQLHQLRGRVG
                     RSRERGYAYFLYSPEKPLTETAHDRLATIAAHTDLGSGMAVAMKDLEIRGAGNLLGGE
                     QSGHIAEV"
BASE COUNT          869 a          644 c          725 g          826 t
ORIGIN      
        1 taatggtttg tgatgcagcg ggcaaagaat taactttcaa taatcccgac acatttgtac
       61 caacggcatt tttcggccat gcacaatttg ttcaagccgc gatcaagtct ttagcgtaga
      121 actatgagtc tgaatctaat tactactgcg cttttggcag atgaaaaagt tcagactgca
      181 cttgagttaa ttaagaaaaa acaatcggtt gaatttggta ttccacaaac tgcgatgccg
      241 tatttagccg ggctgattgc gaaaaattca acaggctcgc taattatttg tgcgaccgat
      301 cggattgctg aagatgttgc ggcgcaacta gccgagtatg tccctgcaaa tcaagttgct
      361 ttttttcctg cttgggaaac attgccgcat gaacgtctaa gtccaagtgg agacacaatt
      421 ggcactcgga tggcaatctt gcgcaaactt gctaatccac aagaggctgg tgagataaat
      481 tacttagtta cttcagttcg cgccgtgatt cagccaatgg ctagcggcct aggcaacatt
      541 gcaccactag ttattaatga aggccaaact atggcgcaag aaaaagttat tgagcaactt
      601 agctatttgg gttacgaacg agttgaactt gtagatcgcc gtggtcagtt tgcagtgcgc
      661 ggcggaatta tcgatgtgtt tccaccgtca caaccacacc cagttcgtct tgaatactgg
      721 ggcgatgatg ttgatgacat cagaatgttc tcagtagcag atcagcgctc acttgaccta
      781 gagatagatc aactttttgc tacaccgtgc cgtgaacttt taattactga ttcggttaaa
      841 aagaaagcac aaaagtttgc aatcgagcat ccagaatttg ccgaagtatt tgaaaaagtt
      901 gcggccggga ttgccaccga aggattagaa agttttattc caattctggc tccagcactt
      961 gagacgctat ttgatttagt gccacaaact tggactattc aagaacttga acctgagcga
     1021 attgctactc gggcgcaaga gctgattgat accagcgcag aatttcttgc ggcttcatgg
     1081 ggtaacacca ccgcgggtaa tgcgattccg gttgatttag gtcgcggttt ttatggttac
     1141 cgagaattaa aaagtgaata cttaaatcga gtttggatta gtcaatctgc gctaatgggt
     1201 ccagaaacaa tcacacctga tttggagcca actcctgatt accgtgggca atttgattcg
     1261 ttagttactc aaattaattc ctggtggcaa caagaattcc gggtcgttat ttcaatggca
     1321 gctcatggca cggcagagcg cttagttaat tcacttggtg aattcaacat agcggcgagg
     1381 ttagctacgg agcctgagcc gatcgttaaa cgagttgaag taattactgg tcatcaagaa
     1441 aacggattag tttttaacgc tggcaaatta gttttcttaa ctgaaacaga tttatttggt
     1501 aatcggtcaa ctactaaatc agcccgagct atgccaagca ggcggcgcac cgcaattgat
     1561 ccgctaacac taaagaccgg cgactttgtc gtgcatgaac aacatggtgt tggtaagtac
     1621 attgaagtgg cgcaacgtgt tgttcagggt gctacccgcg agtacttggt aattgaatat
     1681 gcatcgagta aacgcgggca acgtggtgat cgactttatg tcccaactga tcaattagat
     1741 ctagtcacca aatacgtggg tggtgaactg ccaacactta atagacttgg tggtggtgac
     1801 tgggcccaaa ctaaatcacg cgccagacgc gcagtacgac agattgctgg cgaactgatc
     1861 agactttatg ccgtgcgtat ggcaagtaaa ggtcatgctt tcgcacctga ttcaccttgg
     1921 caacaagaac tagaagacgc attcgagtat gtcgaaactc cggatcaact cagcacaatt
     1981 aatgaagtta aagctgacat ggaacgcgat gtgccaatgg atcgcttagt ttgcggtgat
     2041 gttggttatg gcaaaaccga aatcgctgtt cgagctgcat ttaaagcggt gcaagatgga
     2101 aaacaagttg ccgtcttggt accaactaca ttgcttgtgc aacaacacat ggaaactttt
     2161 gctcgtcgat atgcgcaatt cccggtcaag ttagccgcac tttctagatt tcaaactgac
     2221 gcagaagtta agaaaacatt tgccggaatt caagatggaa caatcgacat tgttattggt
     2281 acgcatcgat tatttaatcc agaagttaga ttcaaagatt taggtctagt aattgtcgat
     2341 gaagagcagc gctttggcgt tgaacataaa gaacacttaa aagccttgcg ggcaaatgtc
     2401 gatatgttga ccatgtctgc aacacccatt ccgcgcaccc tggaaatggc ggtaaccggg
     2461 ttacgcgaaa tgtctacaat ccaaacacca cccgaagagc gtcacccagt acttacttcg
     2521 gttggaccgt atgacgagaa atacatagtt gcggctatcc accgtgaatt aattcgtgat
     2581 ggtcaagtgt tctttgttca taatcgggtt gaaagtatta accgagtcgc aagtcgatta
     2641 agtgaattga ttccagaagc tcgaattgca gttgcccacg gccaaatgaa cgaaaagcaa
     2701 cttgaacaga tcattgttga tttttggcag cataagtttg atgttttagt atcaaccaca
     2761 attattgaaa gtggtttaga cattcctaat gccaatactt tgattgttga tcgagcagat
     2821 acatttggcc tttcccagtt acatcaatta cgcggccgag taggccgaag tcgtgagcgt
     2881 ggttacgcct acttccttta ttcacccgaa aaaccgttga ctgaaaccgc gcatgaccga
     2941 ctagccacaa ttgctgcgca tactgattta ggttcaggta tggcggtagc catgaaagat
     3001 cttgaaattc gtggtgccgg taatttactt ggcggcgaac aaagtgggca tatcgccgaa
     3061 gttg
//
LOCUS       DBNHVN010000100         1215 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1058517, whole genome shotgun sequence.
ACCESSION   DBNHVN010000100 DBNHVN010000000
VERSION     DBNHVN010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1058517"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="AC16Y1_01855"
     CDS             complement(<1..296)
                     /locus_tag="AC16Y1_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR5831241.1"
                     /translation="MTGLEILLASLVVASIFGIYRAKTDGHVKEKSEEHIWSTHNCNI
                     DLGEKATLLQFSSSFCQPCRATKLLLRDIAGGIPGVAHVELNAEEHLPLVRELG"
     gene            503..931
                     /locus_tag="AC16Y1_01860"
     CDS             503..931
                     /locus_tag="AC16Y1_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015788023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HIR5831242.1"
                     /translation="MNVKVDPRGPRFGAAITTTVLALVLLTAPSNLATGLLIAQTIVF
                     GLGAFAGLQFHPYGWVFRTFVRPKLSPPLEMEDSAPLRFAQLVGFLFAVVAVVATFGS
                     VENVALVATGFALGAAFLNAAFNICLGCEMYLLGKRLSAK"
     gene            complement(938..>1215)
                     /locus_tag="AC16Y1_01865"
     CDS             complement(938..>1215)
                     /locus_tag="AC16Y1_01865"
                     /inference="COORDINATES: protein motif:HMM:NF024773.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR5831243.1"
                     /translation="QDILGQKVYSSLSELPIVPDIIDVFRKSEDMPKVFEKEFPAISK
                     NCAGKTWWMQLGISNSEVADQASSAGMNVVQDRCIKVDYLNLVTTTR"
BASE COUNT          317 a          248 c          256 g          394 t
ORIGIN      
        1 atccctaatt cgcgaacaag tggaaggtgt tcttcggcgt taagttcaac atgggcaact
       61 cccggaatgc cgcctgcaat atctcgaagc agtaatttag tagcccgaca aggctggcaa
      121 aacgatgagg agaattgaag cagggtagct ttttcaccca aatcaatatt gcaattatga
      181 gttgaccaaa tgtgttcttc tgatttttct tttacatgac cgtcggtctt tgcccgataa
      241 attccaaaaa tagaggcgac aactaggctt gcaagtaata tttctaggcc ggtcatgact
      301 gtcgctttcg ggtaagtagt tccttaaccg taattgaatc ttgaactaat tgaaaattga
      361 aaccaaattt gattactgca aaagttttgc aattgagttg ttattgctta gctaaaaata
      421 ttttttcccg tatctttatg ttctatcgtc agcttaattt gttgatagta attaattgtt
      481 tgcagcctga aagaagtaat tgatgaatgt aaaagtggat ccgcgtggac caaggtttgg
      541 cgcagcaatc accacgacag ttcttgcatt agttttactg accgcgccat caaatctggc
      601 tactggctta cttattgcac aaacgatagt tttcggtctg ggcgcttttg ctggactgca
      661 gtttcaccca tatggctggg ttttccgaac tttcgttcgg ccaaaacttt caccgccact
      721 tgaaatggaa gattctgcgc cacttcgatt tgctcagtta gtaggttttt tgttcgcagt
      781 agttgcagtt gttgccactt ttggctcagt cgaaaatgtt gcattggttg caacaggttt
      841 tgctttaggc gctgcatttc taaacgctgc ttttaatatc tgcctaggtt gcgaaatgta
      901 tctcttaggc aagcgtttat ctgcaaaata aatccgatta acgtgttgta gtaactaggt
      961 tcaaataatc gactttaata cagcgatctt gcacgacatt cattccggcc gaacttgctt
     1021 ggtcggcaac ttcgctattt gagataccta actgcatcca ccaggtttta ccagcgcaat
     1081 ttttggaaat ggccggaaat tctttttcaa aaacttttgg catgtcttcg ctttttcgaa
     1141 atacatcaat aatgtctggc actatcggta actccgagag actcgaataa accttttggc
     1201 caagtatgtc ttggg
//
LOCUS       DBNHVN010000101         3447 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_463281, whole genome shotgun sequence.
ACCESSION   DBNHVN010000101 DBNHVN010000000
VERSION     DBNHVN010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_463281"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..1078
                     /gene="rpsB"
                     /locus_tag="AC16Y1_01870"
     CDS             284..1078
                     /gene="rpsB"
                     /locus_tag="AC16Y1_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR5831244.1"
                     /translation="MAVISTRQLLENGVHFGHQTRRWNPKMKRFIFTERNGIYIIDLQ
                     QSLAFIDRAYEFVRETVARGGSVLFVGTKKQAQEAIAGQATRVGMPYVNHRWLGGMLT
                     NFATVAKRVARLKELEAINFDDTAGTGLTKKELLVLRREKEKLELVLGGVRDMQKLPS
                     AIWVVDTNKEHIAVGEARKLGIPVVGILDTNCDPDVVDFPIPGNDDALRAVGLLTGVI
                     ADAVAEGLIARSGSAPAGTTEAEPLAEWEKELLETSATTPAQTPAE"
     gene            1177..1989
                     /gene="tsf"
                     /locus_tag="AC16Y1_01875"
     CDS             1177..1989
                     /gene="tsf"
                     /locus_tag="AC16Y1_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR5831245.1"
                     /translation="MSISAADVKKLRDVTSAGMMECKKALEEANGDFDKAVEILRISG
                     AAKAAKRGAERETSAGLVANDGGVLVELLCETDFVAKNEDFSKLANQIVAAVKAAGAK
                     DSAEASNLKLDGGDTVAQAIEKMAGVIGEKLELGRVANREGDVAVYLHRRSNDLPPAV
                     GVLVNYSGSEDVARSVAMQIAAMRPGYLTRDEVPAEVVETEKRIAAETAKEEGKPEAA
                     LPKIVEGRVNSFFKDNCLLEQSSVQDNKKTVKQMLDEAGTTVTGFSHFEIGK"
     gene            2180..2911
                     /gene="pyrH"
                     /locus_tag="AC16Y1_01880"
     CDS             2180..2911
                     /gene="pyrH"
                     /locus_tag="AC16Y1_01880"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005295274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR5831246.1"
                     /translation="MWPGVPANGWNRVLLKLSGEVFAGEGKLGVDPNVVADIARQIAE
                     VVKTKTEVAVVIGGGNYFRGAELSERGMDRARADYMGMLGTVMNCLALQDFLEKQGID
                     TRVQTAIPMSQVAEPYVPRRAARHLEKGRVVIFGAGLGAPFFSTDSCAAQRALEIGAQ
                     VVLMAKGVDGVYDSDPRTNPKAKFYETLSHDEVLSQNLKVADATAISLCRDNKLPIVV
                     FNLLVDGNIARAVRGERIGTLVSTN"
     gene            2924..>3447
                     /gene="frr"
                     /locus_tag="AC16Y1_01885"
     CDS             2924..>3447
                     /gene="frr"
                     /locus_tag="AC16Y1_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017235920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR5831247.1"
                     /translation="MVIENILKDAQEKMDKAIEVTREDFLTVRAGRASAALFSKVMVE
                     YYGTMTPIMQVASIQVPEARTAVVTPFDKSALGAIERAIRDSDLGVNPSNDGVVIRVP
                     FPQLTEERRKEFIKVAKTKAEDSKVSIRNIRRHAKESLEKLAKDGDAGKDDVARAEKT
                     LEDLTTKTVAHVDE"
BASE COUNT         1029 a          712 c          862 g          844 t
ORIGIN      
        1 atgaggttcg ttatgtcagt tattcgggaa taacgccaga gcgaaaattg gcttggggaa
       61 atccgattaa tgtgcatttc taggcggtat ttgactggat taaaacccac taaactatta
      121 attcgcacct aggaataggt gacatcacag gttcaacaag cgatctgaaa gaccatcggt
      181 cttgcttagg caagcggact ccagaagcag aaccaagggc acaaccaagt gcacaaccgc
      241 aatgaggtta gtgaaaactg acctaagaaa taggagtaat ccaatggccg taatttcgac
      301 cagacagctt ctggaaaatg gtgttcattt cggacatcaa acccgtcgct ggaatccaaa
      361 gatgaagcgt ttcattttta ctgaacgcaa tggcatttac atcatcgact tacagcaatc
      421 acttgctttc atcgatcgcg cttacgaatt cgtgcgcgaa actgtggcac gtggtggttc
      481 agtactattc gtcggtacca agaagcaagc tcaggaagcg attgctggcc aagcaactcg
      541 cgttggtatg ccgtatgtta atcaccgctg gttaggtggc atgttaacta acttcgctac
      601 tgtggcaaag cgcgtagctc gtcttaaaga attagaagca attaattttg acgacacggc
      661 tggtactggt ttgaccaaaa aagaattatt agtacttcgt cgtgaaaagg aaaaacttga
      721 attagtactt ggtggcgttc gcgacatgca aaaactacca agtgcaatct gggttgtaga
      781 taccaacaaa gaacacattg cagtcggcga agcacgcaaa ctaggtatcc cagttgtggg
      841 catcctggac acaaactgtg atccagacgt tgttgatttc ccaattccag gaaatgatga
      901 cgcacttcgc gccgttggtt tactaactgg cgtgattgca gatgcagttg ctgaaggttt
      961 aattgctcgc tcaggcagtg cgccagcagg cactaccgaa gcagaaccac tggctgaatg
     1021 ggaaaaagaa cttcttgaaa ccagtgcaac aacgccagct caaacacctg ctgaataaat
     1081 ctcaaagtca gccaagtagt aattactact tggctgactt aaaactgcaa gaaaataaaa
     1141 actttaactg ctaactaact aattaaggat taacagatgt ctatctcagc cgccgacgtc
     1201 aagaaactac gtgacgtaac ttctgccgga atgatggagt gcaagaaagc gctagaagaa
     1261 gccaatggcg acttcgataa ggccgtagaa attttacgta tctctggtgc cgcaaaggca
     1321 gcaaagcgtg gtgcagagcg cgaaacttca gccggattag ttgctaatga tggcggcgtt
     1381 ttggttgaac tactttgcga aacagacttc gttgcaaaaa atgaagattt ctcaaaatta
     1441 gccaaccaaa ttgttgctgc ggtaaaagca gcaggtgcaa aggacagcgc cgaagctagc
     1501 aacttaaaac tcgatggtgg cgatactgtc gcacaagcca ttgaaaaaat ggctggtgtg
     1561 atcggtgaaa aacttgagtt aggccgggta gcaaatcgcg aaggcgacgt tgctgtttac
     1621 ctacaccgtc gctcaaacga tctaccacca gcagttggtg tcctggttaa ttattcaggt
     1681 agcgaagatg tggcgcgcag cgttgcaatg cagattgctg ccatgcgccc aggttaccta
     1741 actcgcgatg aagttccagc tgaagtagtt gagacagaaa agcgtattgc tgcagaaact
     1801 gctaaagaag aaggcaagcc agaagcagcc ctgccaaaaa ttgtcgaggg tcgggtaaat
     1861 agtttcttta aagataattg cctactagag caatcatctg tgcaagataa caagaaaact
     1921 gtgaagcaaa tgttggatga agctggcaca acagtgactg gctttagtca ttttgaaatt
     1981 ggcaagtaac cctaaataac taagtgccag ggcagcctaa atggttgccc tggttatttt
     2041 ttgggcagat tttcatcaga aattggggta agtgataact cgtttcggta ttagtgccgg
     2101 gcaagtttgg aatcgacttt agatacggca aaatagaggc gtggctaccg cagtttcaaa
     2161 ttccgatgta gccaaaggca tttggccggg tgtgccagcc aatggttgga accgagtttt
     2221 actaaaacta tctggtgaag ttttcgctgg tgaaggcaaa cttggcgtag acccgaacgt
     2281 agtcgccgac attgccagac aaatcgctga agtagttaaa acaaaaaccg aagttgccgt
     2341 ggttatcggt ggcggtaact acttccgcgg agcggaatta tctgagcgcg gaatggatcg
     2401 agctcgagct gactacatgg gcatgctcgg tacggtgatg aactgtttag cgttacaaga
     2461 tttcctagaa aagcagggaa ttgatacgcg ggtacaaact gctatcccga tgagtcaggt
     2521 ggctgagcca tatgtacctc gccgtgctgc tcgacattta gaaaaaggcc gagttgtaat
     2581 ttttggcgct ggtttaggcg ctccattttt tagtaccgac tcttgtgccg cccagcgcgc
     2641 acttgaaatt ggtgcccaag ttgttttgat ggcaaaaggt gttgatgggg tttatgacag
     2701 tgacccacgc actaatccaa aagctaagtt ttatgaaaca ctttcgcacg atgaagtttt
     2761 gtcgcagaat ctgaaagttg cagatgccac tgcaattagt ctctgccgag ataacaagtt
     2821 accgattgta gtttttaatt tattagtaga tggaaatatc gctcgtgccg ttaggggcga
     2881 gcgtatcggc accctagttt caaccaatta aagatacgag gaaatcgtga ttgagaatat
     2941 tttgaaagat gcccaagaaa agatggataa agccatcgaa gtaactcgtg aagattttct
     3001 aaccgtgcgg gctggccgag cgtccgcagc gttattttca aaagtaatgg ttgagtatta
     3061 cggcactatg actccgatca tgcaggtggc atcaattcaa gtacctgaag cacgtactgc
     3121 agtagtgact ccatttgata aaagtgcgct aggtgcgatc gaacgagcca ttcgagactc
     3181 agatcttggg gtcaatccat ctaacgatgg agttgttatc cgggtgccat tcccacaatt
     3241 gaccgaagag cgccgcaaag aatttattaa agtagcaaaa actaaagcag aagattcaaa
     3301 agtgtctatc cgaaatatcc gtcggcatgc aaaagagtct ttagaaaaac ttgctaaaga
     3361 tggcgatgct ggcaaagatg acgtggctcg agcagaaaaa acactggaag atttaacgac
     3421 aaagactgtc gcccatgttg acgaaat
//
LOCUS       DBNHVN010000102         3742 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_610706, whole genome shotgun sequence.
ACCESSION   DBNHVN010000102 DBNHVN010000000
VERSION     DBNHVN010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3742)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3742)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3742
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_610706"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="AC16Y1_01890"
     CDS             complement(<1..545)
                     /locus_tag="AC16Y1_01890"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase family protein"
                     /protein_id="HIR5831248.1"
                     /translation="MAAIDQNELNPKIWPDSARRSDFGELTIGGVGVSALVKEFGSPL
                     FIFDEDHVRNRAKSYLAAFNNQQIPTDVYYASKAFLSTKVAKWLTDEGLGIDVCTDGE
                     LEVALRAGVRPEQILFHGNNKSVAELEKAVKVGVGRIVVDSLIEIERLNQIAAVNGVT
                     VQVLVRITVGVEAHTHEAIATA"
     gene            complement(548..2227)
                     /gene="argS"
                     /locus_tag="AC16Y1_01895"
     CDS             complement(548..2227)
                     /gene="argS"
                     /locus_tag="AC16Y1_01895"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR5831249.1"
                     /translation="MTPQELVKHIETAVKSAISANQLELTVAEIPQEIVVERPKNPEH
                     GDWATNIALQISKKAGLNPRAVAEILLPLIAKVAGVSDAQIAGPGFINIKISAGSQGQ
                     IAASIVKQGEKYGHGVKLSGKKINVEFISANPTGPLHLGHTRWAAVGDAIARVLEAEG
                     AQVAREFYINDRGVQMDKFGGSVKSAALKETIPEDGYHGAYIADLANEIIGNQPELIN
                     LPEPERSVAFREAAYQLQLADQKRVLALFNTHFDVWYSERTLHESGAVERAFTKLKAQ
                     GHMFELDGATWLRTTDFGDDKDRVLVKADGELTYFASDTAYYVDKRARGFDNCIYLLG
                     ADHHGYVNRLRAVCACAGDNPDENIEPMIGQMVKMMKNGQEVRLSKRAGNIITLEDLV
                     DEVGVDAARYSLIRYPVDSPLVLDLDLLVSRTNDNPVYYVQYAHARIASVVRNAIELG
                     IMTATDWDVTKFDPNLLVDERESQLLGLIADYPRIVATAAELRQPHRIARFMEEVATS
                     YHQFYAACRVLPQGDEPASDLTIARLWLCAATRQTIFNGLSLLGVSAPERM"
     gene            complement(2285..2932)
                     /locus_tag="AC16Y1_01900"
     CDS             complement(2285..2932)
                     /locus_tag="AC16Y1_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR5831250.1"
                     /translation="MNGPISSQESVLNELREMIGSGLLAPGQQIVQESLSVSLGVSRV
                     PIREALRVLEGEGQVVHFPNRGYFVASLSVEDLEEVYRIRAILEDEALRIAVPLLTDQ
                     DIDYIEAILTEVDEAAKSRKISDLTETNRRFHFAIFEASNMPRLVRMIRTLWDATDAY
                     RGVYFASPTNLNEMDHEHRDMVTALRERDVEKIVQLQGVHRANSVSAVSQIIRRK"
     gene            complement(2947..>3742)
                     /locus_tag="AC16Y1_01905"
     CDS             complement(2947..>3742)
                     /locus_tag="AC16Y1_01905"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR5831251.1"
                     /translation="YEAVSLINANVVYCAISGFGSKAGAAIPGYDLLVQAMGGLMSVT
                     GSRPDEPSKVGAALVDVITGLHATIGILAALNHRKETGDGQSIEVNLLSSVLSGLVNQ
                     ASAYVSGGVIPSSLGNAHPSISPYEVYPTADRPIVLAVGNDGQFAKLCNALGCPEVSA
                     DVRFESNANRVANRAALNEILIAHLVKNGADFWWKKLTDADVPCGPINNMAEAFELAK
                     QMELDPIIEINQDGEIRKQVANPIAFSKTPVEYRTAPPAIGEDFTK"
BASE COUNT         1012 a          917 c          729 g         1084 t
ORIGIN      
        1 gcagttgcaa tggcctcatg agtatgagct tctaccccaa cagtgatccg caccaatact
       61 tgcacagtca ccccattgac ggccgcgatc tggtttaacc tttcaatttc aatcaagctg
      121 tctacgacaa ttcgaccgac gccaactttg actgcttttt ctaattcagc aacgctttta
      181 ttgtttccat gaaacaaaat ttgctctggt ctaacaccgg ctcgaagggc aacttctaat
      241 tcaccgtcag tacagacgtc gattcccagt ccttcatctg ttaaccattt agctactttc
      301 gtggaaagaa atgctttact tgcatagtaa acgtcagttg gaatttgctg attattaaac
      361 gcggctaagt aacttttagc tcgatttcgt acgtggtctt catcaaaaat aaacagtggc
      421 gaaccaaact ctttaactaa tgcgctaacc ccaaccccac caatagttaa ctcaccaaaa
      481 tcacttcgac gtgctgagtc aggccagatc ttcggattta attcattttg atcgatagcg
      541 gccataacta cattctttct ggagcagata cgccaagtag tgacaagcca ttaaaaattg
      601 tttgcctggt tgccgcacaa agccataagc gagcaattgt taaatcactc gctggctcat
      661 caccctgtgg aagtacccga caggcagcat aaaactgatg gtaagaagtt gccacttctt
      721 ccatgaacct agcgatgcga tggggttgtc gtaattcggc agccgttgcc acaatgcggg
      781 ggtaatcggc gattaatcca agtaactgac tctcgcgttc atccactaac aagtttggat
      841 caaacttggt tacatcccaa tctgtggcag tcattatgcc cagctcaatt gcatttctaa
      901 ccactgaagc tatccgcgcg tgggcatatt gaacgtaata aaccggattg tcattagttc
      961 tagaaactaa taaatcaaga tccagtacca atggcgaatc aactgggtac cttattaatg
     1021 agtagcgtgc tgcgtctacc ccaacttcat cgactaaatc ttctagcgtg atgatgttgc
     1081 ccgcacgttt tgataagcga acttcttgac cgtttttcat cattttgacc atctggccaa
     1141 tcatcggttc aatattttca tctggattat caccagcaca tgcacaaact gcacgcaatc
     1201 tatttacata accgtgatga tcagcaccta gcaagtaaat acagttatca aaaccgcggg
     1261 ctcttttatc cacatagtaa gcagtgtcag atgcaaagta agttaattca ccatctgctt
     1321 taactaaaac tcgatctttg tcgtcgccaa aatccgttgt tcgaagccaa gttgctccat
     1381 ctagttcgaa catgtgtccc tgagctttga gtttggtaaa ggcgcgctca actgcaccac
     1441 tttcatgtaa ggttctctcg ctgtaccaaa catcaaagtg cgtgttgaat aaagcgagta
     1501 ctcgtttctg atctgcaagt tgcaactgat aagccgcctc gcgaaatgcg acgctacgtt
     1561 ctggttctgg taaattaatc aactccggct ggttgccaat aatttcattt gctaaatctg
     1621 cgatgtaagc gccgtgatat ccatcttctg gaatagtctc ctttagcgca gctgatttaa
     1681 ctgagccgcc aaacttatcc atttgaacgc cacgatcgtt tatgtagaac tcgcgggcta
     1741 cttgtgcacc ctcggcttcg agaactcgag cgatcgcgtc accaacggct gcccaccgag
     1801 tgtgtcctaa atgcagtggg ccagttggat tcgccgaaat aaattcaacg ttgatttttt
     1861 tgcccgaaag ctttacaccg tgaccgtatt tttcgccttg cttaacaatg ctggcagcta
     1921 tttgtccttg gcttccagca gaaattttta tgtttataaa accaggtcca gcaatttgtg
     1981 catcactaac tccggcaact tttgcaatta gtggaagcaa aatttctgca actgcacgcg
     2041 gatttaagcc tgctttttta gaaatctgaa gggcaatatt tgttgcccaa tctccatgtt
     2101 caggattttt cggtcgctcg acaacaattt cctggggaat ttccgcaaca gttaattcaa
     2161 gttgatttgc cgaaatggcg ctcttgaccg cagtttctat gtgcttaact aattcctgcg
     2221 gcgtcatacg ccaaaagcct accgaaaagt tttgagttta aagaattttt ggcagtacgt
     2281 gaagttactt tcggcgaatt atctgactta ctgcgctaac cgaattagcg cgatgtactc
     2341 cttgaagctg cacaatcttc tcgacatcac gttcccgaag tgcggtaacc atgtcacggt
     2401 gttcatgatc catttcattt aagttcgttg gcgaagcaaa gtaaacacct cggtaagcat
     2461 ccgttgcatc ccaaagcgtc cgaatcatcc gaactaagcg cggcatgtta cttgcctcaa
     2521 aaattgcaaa atgaaaacgc cgatttgtct ctgttaaatc tgagatcttt cggctttttg
     2581 ctgcctcatc aacttcagtc aatattgctt ctatgtagtc gatgtcttga tcggttagga
     2641 gcggaactgc aattcgaagc gcttcatctt caagaatcgc tcgaattcga taaacctctt
     2701 caagatcttc aacagacaaa cttgctacaa aatatccacg atttggaaag tgaacgacct
     2761 gaccttcgcc ctcgagaact cgcaatgctt ctcggatcgg aactcgactc acaccaagag
     2821 aaactgatag agactcttgc actatttgct gtcccggggc caataagcct gaaccaatca
     2881 tttcccgcaa ttcgttcaac acagattctt gcgatgagat aggaccgttc attcgttatc
     2941 cattcattac ttagtgaaat cttcacctat tgcaggtggt gcagttcgat attcaacagg
     3001 tgttttactg aaggctatcg gatttgcaac ttgctttcga atttcaccat cttggtttat
     3061 ctcgataatt ggatcgagtt ccatttgttt tgcaagttca aatgcctcgg ccatattatt
     3121 tattggccca catggcacat cagcatcagt cagttttttc caccaaaagt ctgcaccatt
     3181 ttttaccaaa tgtgcaatta aaatctcgtt gagcgcagcg cgatttgcaa ctcggtttgc
     3241 atttgattca aagcgaacat ctgctgaaac ttcagggcaa cctaatgcgt tacaaagttt
     3301 tgcaaattga ccatcattac caactgctaa aactattggg cgatctgcag ttggataaac
     3361 ctcatacggg ctaatcgaag ggtgagcatt accaagtgaa cttggaatga ctcccccaga
     3421 aacatatgcg ctggcttgat ttactagtcc cgacaaaact gaactcaata aattcacttc
     3481 aattgactgg ccatcaccag tttcttttcg atgatttagc gccgctaata ttccgatcgt
     3541 ggcatgtagc ccagttatga catcaactaa tgctgcacca acctttgatg gttcatctgg
     3601 tcttgatcca gttacgctca tcaagccacc cattgcttga actagcaagt cgtagccagg
     3661 aattgcagct ccagctttcg agccgaaacc agatattgca caatagacaa cattcgcatt
     3721 aattagactt acagcctcat aa
//
LOCUS       DBNHVN010000103         2576 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_537975, whole genome shotgun sequence.
ACCESSION   DBNHVN010000103 DBNHVN010000000
VERSION     DBNHVN010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_537975"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="AC16Y1_01910"
     CDS             complement(<1..494)
                     /locus_tag="AC16Y1_01910"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR5831252.1"
                     /translation="MRIARVSVPELTGPVFGVIDGTKIEIIKGHPFAEIEKSGIEFDL
                     DQVKILSPVLPSKVVCVGKNYADHARELGGEVPAEPVIFLKPSTTVIGEGDPIVLPSQ
                     SQNVHHEAELAVVISHLCKNVEIDRAAEVILGYTVANDVTARDLQESDGQWTRAKSFD
                     TFCPL"
     gene            complement(528..2102)
                     /gene="cimA"
                     /locus_tag="AC16Y1_01915"
     CDS             complement(528..2102)
                     /gene="cimA"
                     /locus_tag="AC16Y1_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015746778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HIR5831253.1"
                     /translation="MLSDNFHVYDTTLRDGAQREGISYSVEDKLTVAKLLDEYGVGFI
                     EGGWPGALPKDTEFFARMAAGEVKLKNAKLVAFGSTRKAGVSANEDPQVKALVDSQAP
                     VITLVAKSDVFHVEKALRTTPEENLAMISDTVSYLVGLGRRVFVDFEHFFDGYKHDSD
                     YGVAAIKAAITAGADVAVLCDTNGGMLPSGISKIVTDVKERTGARIGIHCQDDTGCAI
                     ANSIAAIEAGATHVQCTANGYGERTGNADLFVVVPNIELKLGQKVLPEGALAETLRIA
                     HAIAEIANLPPNTHQPYAGVSSFAHKAGLHASALKVNYDLYNHIDATLVGNDMRVLVT
                     EMAGRASVELKGKELGYDLTSNPNAINEVVDKVKSLEARGWSFEAADASFELLLQNAL
                     NGDGYKKFTVESWKTITEQRENGQVVSEATVKLQVNGERIISTGEGNGPVNALDNAFR
                     SALVQRFPELESLDLVDYKVRILDGGAGTGAVTRVLIETADGKKRWTTVGVHENVIAA
                     SWLALEDAINYGLLSN"
     gene            complement(2111..>2576)
                     /locus_tag="AC16Y1_01920"
     CDS             complement(2111..>2576)
                     /locus_tag="AC16Y1_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018383498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR5831254.1"
                     /translation="QSEAAAQGCDQVVWVDAKERKYIEEMGGMNLFFVYGKGKDARVV
                     TPQLTGTLLPGVTRDSLLTLAAELGYQADEVKITIDEWRAGCASGEITESFACGTAAV
                     ITPVGSVDSKNSGTWTIGDGQPGEITMKLREALVNIQTGRAEDTHNWMHRLV"
BASE COUNT          683 a          647 c          509 g          737 t
ORIGIN      
        1 agcggacaaa atgtgtcaaa actctttgct ctggtccact ggccatcact ttcttgtaag
       61 tcacgtgcgg tcacatcgtt ggcgactgtg taacccaaaa tcacttcggc agcccgatca
      121 atttcaacat ttttacataa gtggcttatc actacagcta actcagcttc atgatgaaca
      181 ttttgcgatt gacttggcag cacaatcgga tcgccttcgc cgatcactgt ggttgatggc
      241 ttcaaaaaaa taactggctc ggctggtact tcgccaccaa gttctctggc atgatctgca
      301 taattttttc caacgcatac aactttgctt ggcaaaacgg gcgaaagtat ttttacttga
      361 tctagatcaa actcaatccc tgatttttca atctcagcaa atggatgacc tttaattatt
      421 tcaatttttg taccatcgat aaccccgaaa acaggtccgg ttagttcagg tacagaaacc
      481 cgggcgatac gcattagtaa agaataccaa tcgaattaat ttgcaatcta attgctcaat
      541 aagccgtaat tgatcgcatc ttcaagcgct aaccacgaag cagcgatcac attctcatgt
      601 acgccaacag tagtccaacg cttttttcca tcagctgttt caattagcac tcgagttact
      661 gcgccagtac ctgcgccgcc gtccaatata cgaactttgt agtcaactaa atctaaggac
      721 tcaagctctg ggaagcgctg cactaaggca cttcgaaagg cattatctag tgcattaacg
      781 ggaccattgc cttcgccagt tgaaattatt cgctcaccgt ttacttgcaa tttaaccgtt
      841 gcttcactta caacttggcc attttcacgt tgttctgtaa ttgtcttcca agattcaacc
      901 gtaaattttt tatagccatc accgttaagt gcattttgga gtagcaattc aaaggaagca
      961 tcggctgcct caaatgacca gccacgagct tcaagacttt taactttgtc cactacttcg
     1021 tttatcgcat tggggttact agttaagtca taaccaagtt ctttaccttt tagttcaacg
     1081 gatgcgcggc ccgccatttc ggttaccagc actcgcatat cgttaccaac caaagtggca
     1141 tcaatgtggt tatacaagtc atagttaact tttaaggcgc tggcatgtaa gccggccttg
     1201 tgcgcgaatg aagaaactcc agcgtatggc tgatgagtat ttggtggcag gttggcgatt
     1261 tcagctattg cgtgtgcgat acgcaatgtt tcggctagtg caccttcagg caatactttt
     1321 tggccaagtt ttaattcaat atttggcacc acaacaaaaa gatcggcatt gccggttcgt
     1381 tccccataac cattagcagt gcactgcaca tgagttgcgc cagcttcaat tgcggcaatt
     1441 gaattagcaa tcgcacagcc agtgtcatct tgacaatgaa ttccaatgcg cgcaccagtt
     1501 cgctctttta catcggtcac aattttgcta attccagaag gcaacatgcc accattagta
     1561 tcgcaaagca ctgcaacatc agctccggcg gttattgccg ctttaatcgc tgctacccca
     1621 tagtccgaat catgtttgta gccatcaaag aaatgttcaa aatcaacaaa aacgcggcgg
     1681 ccaagtccta ccaagtacga aaccgtatcc gaaatcatcg ctaagttttc ttctggtgtg
     1741 gtgcgaagtg ctttttcaac atgaaagaca tcgctctttg ccacgagtgt gattaccggt
     1801 gcttgtgaat caacaagagc tttaacttgc ggatcttcgt tcgcagaaac tccggcttta
     1861 cgagtagagc caaatgcaac aagttttgca tttttaagtt ttacttcacc agcagccatt
     1921 cgggcgaaaa actcggtgtc ttttggcaag gcgccgggcc agccaccctc aataaaacca
     1981 acaccatatt catcgagtaa ctttgccaca gttaatttgt cttctactga atatgaaata
     2041 ccttcgcgtt gagcaccatc gcgaagcgtg gtgtcgtaaa catgaaaatt gtcgctcaac
     2101 atttctgtac ttaaaccaac ctgtgcatcc agttatgggt atcttcagcc cgaccggttt
     2161 gaatgtttac cagagcttcg cgtaacttca ttgttatttc gcctggctga ccgtctccga
     2221 ttgtccaagt tccactattt ttactgtcta ccgaacccac aggtgtaata actgcagccg
     2281 ttccgcatgc aaatgactca gtgatttcac cagatgcaca accagcgcgc cattcatcaa
     2341 tagtgatttt cacttcatca gcttgataac caagttcagc tgccagagtc aacaacgaat
     2401 cacgagttac acctgggagt agggtgccag ttagctgtgg agttactacc cgggcatctt
     2461 ttcctttgcc gtagacaaag aataaattca tgccacccat ttcttcgatg tatttgcgct
     2521 ctttggcatc aacccaaacc acttgatcgc aaccctgtgc tgcagcttct gattgg
//
LOCUS       DBNHVN010000104         1222 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_92751, whole genome shotgun sequence.
ACCESSION   DBNHVN010000104 DBNHVN010000000
VERSION     DBNHVN010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_92751"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..>1222)
                     /gene="secD"
                     /locus_tag="AC16Y1_01925"
     CDS             complement(3..>1222)
                     /gene="secD"
                     /locus_tag="AC16Y1_01925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIR5831255.1"
                     /translation="STAKLTFRQVITFDLGSPAPTPTNTESPSPVPNPSVDPSLLVAP
                     IQSATLDDQLAARFAALDCSKSDVLSGGAIDDETQYLVTCDEDGLYKYVLGPAGLSGT
                     DISDAVAGLPQQGAGGWQVDLTMTSDGAKKFADVTTQLSSQQTPQDQFGIVLDGLVVS
                     APQVNEPILGGSATISGTFTADEARALAQILKYGALPITLNVDEVSQISPTLGNDQLR
                     AGVLAGAFGLLLVVLYLLGYYRALGTVAVGSLIVAALILYFVAVVLGNEINFTMTLSG
                     IAGAIVAIGITADSFIIYFERIRDELREGKRLKAAVDAGWQRARRTILAADFVSLLGA
                     VILYYLSVGNVRGFAFALGVTTLIDIFVAFYFTRPLVVKIAQTKWMAKGSAMTGVSPR
                     RLGFVESTQEVAQ"
BASE COUNT          354 a          302 c          257 g          309 t
ORIGIN      
        1 tttcattgag ccacctcctg ggttgattca acgaagccca atcgtcgggg tgaaactcca
       61 gtcattgcgg agccttttgc catccatttt gtttgggcaa tttttaccac caaaggtcga
      121 gtgaaataaa aagccacaaa gatatcgatc aaagttgtga ccccgagtgc aaaagcaaaa
      181 cctcgcacgt tacctacaga caagtaatac aagatcacgg cacctagtaa agatacaaag
      241 tctgccgcca gaattgtgcg acgagcacgc tgccaacccg cgtctacagc agccttcaaa
      301 cgtttacctt cccgaagttc gtctcggatt cgctcaaaat aaataatgaa ggagtcagcc
      361 gtgataccga ttgccacaat tgctccggca atacctgaga gcgtcatggt gaaattaatt
      421 tcattaccca gcaccacagc aacaaaatat agaattaacg cagccacgat caaactgcca
      481 actgcaactg tacccagagc tcggtagtag cccaaaagat acagaacaac caatagcaaa
      541 ccgaatgcac ccgctaagac tccagcacga agttgatcgt tacctaaagt tggtgaaatt
      601 tgcgaaacct catccacgtt aagtgtgatc ggcagcgcac catacttaag tatttgcgcc
      661 aatgctctgg cttcatcagc agtaaatgtt ccactaatgg ttgcactgcc accaagaatt
      721 ggttcgttca cttgcggtgc gctcacaact aatccgtcta agacgattcc aaattgatct
      781 tgtggtgttt gttgacttga taattgggta gttacatcag caaatttctt tgctccatcc
      841 gaagtcatcg ttaaatcgac ttgccagcca ccagcaccct gttgcggtaa tccagcaact
      901 gcatctgata tgtcggtgcc tgataaacct gctgggccta gtacatattt ataaagtcca
      961 tcttcatcac aagtgaccaa atactgagtt tcgtcatcta ttgccccgcc gcttaaaaca
     1021 tccgattttg aacaatccaa tgcggcaaat cttgcagcca gttgatcgtc aagagttgca
     1081 gattgaatcg gcgcaactag aagtgatgga tctactgatg gattcggaac tgggctagga
     1141 ctttcggtgt tagtcggtgt aggcgccgga ctgcctaaat caaaagtaat tacttgtcgg
     1201 aaagttaatt tggctgttga tt
//
LOCUS       DBNHVN010000105         2304 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_761874, whole genome shotgun sequence.
ACCESSION   DBNHVN010000105 DBNHVN010000000
VERSION     DBNHVN010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_761874"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1651)
                     /locus_tag="AC16Y1_01930"
     CDS             complement(<1..1651)
                     /locus_tag="AC16Y1_01930"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR5831256.1"
                     /translation="MSDSMALRFGVGIAITVVLLGFALKRLWFLYSLVRSGQPAVGRT
                     AERKRRVKAEVTEVLGQKKLLAWTVPGIAHVFAFWGFIVLGLTIVEAFGALVNPEFAV
                     PLIGRLPVVGFLEDLFAVLVLVGIAMFALIRVFSSPAKYGRDSRFYGSHTGAAWFVLF
                     MIFNVIWTLFLYRGAQWAVSFEKGANNFPFMNGGAFASEAIGRLMLPLGVTTNEWLES
                     IGLWLNIGVILGFLVLALHSKHLHIGAAPLNVLFSRRPDALGELQPIYAGKEVLNFED
                     PADDATFGSGKIEDFSWKANLDLLSCTECGRCQSQCPAWNTGKPLSPKLMIMDLRDHL
                     FAKAPYILAGEDAAKIEKLSDAVKAEAARPLVGSRQDDPLFLTDGYDEKGHRSNVGAV
                     IDYDALWSCTSCGACVQQCPVDIEHVDHFMEMRRHQVLVESEFPAELNGLFKNLETKG
                     NPWGMNQSGRTAWIEEVQFPVRVFGMQGEDKIPADVEYLFWVGCAGAYDDRAKRTTKA
                     VAELLHTAGIEFMVLGEGETCTGDPARRAGNEFLFQMQAQQN"
     gene            complement(1743..>2304)
                     /locus_tag="AC16Y1_01935"
     CDS             complement(1743..>2304)
                     /locus_tag="AC16Y1_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR5831257.1"
                     /translation="LHFDPELLEFGDRRNVVDKYRYWTVEAIVADLDKSRSKLHIAIE
                     NWQHDLNIGSIVRTANAFNVAGVHIVGKRGWNRRGAMVTDRYLHLFHHPEVSDFKKWS
                     LENNVPIIGIDNVPGCADLEDSSLPESCVLYFGQEGPGMSEEGLAACTEILGITQLGS
                     TRSMNASAAGAIAMYAWAMQHLHKNK"
BASE COUNT          697 a          576 c          431 g          600 t
ORIGIN      
        1 cattttgttg cgcctgcatc tggaataaaa attcatttcc agctcgacgg gctggatcac
       61 ccgtacaagt ttcaccttca ccaagcacca taaattcaat gccagctgta tgaagtaact
      121 cagcgaccgc tttagttgtt cgttttgcgc ggtcatcgta cgcaccagca cagcccaccc
      181 agaataaata ttcaacgtca gccggaattt tgtcttcacc ttgcataccg aaaactcgaa
      241 ctgggaattg aacttcttca atccaggcgg tacgtccact ctggttcatt ccccatggat
      301 tgcctttagt ttctaagttc ttaaacaacc catttagttc agctggaaat tctgactcga
      361 ctaaaacttg atggcggcgc atttccataa aatgatcaac gtgttcgatg tctactgggc
      421 attgctgcac acatgcacca catgaagtac atgaccaaag tgcgtcgtaa tcaattactg
      481 ccccgacatt tgatcggtga cctttttcat cataaccatc cgttaaaaac agcggatcgt
      541 cttggcgtga gccaacaagt ggtcgggctg cctcagcttt aactgcgtca cttaactttt
      601 caatttttgc agcgtcttcg ccagcaagta tgtatggtgc tttcgcaaat agatgatcac
      661 gcaaatccat aatcattagt tttggtgaaa gtggtttacc agtattccaa gccggacatt
      721 ggctttggca acgaccacac tcagtgcaac ttaataaatc aagattcgct ttccaagaaa
      781 agtcctcgat ttttccgcta ccaaaggttg catcatcagc aggatcttca aagttaagaa
      841 cttctttgcc agcataaata ggttgtaact cgccaagtgc atcaggtcga cggctaaata
      901 aaacgttcaa aggcgctgca cctatgtgca ggtgcttact gtgtagcgct aaaactaaaa
      961 acccaagaat tactccgatg tttaaccaaa ggccaatact ttctaaccac tcatttgtag
     1021 tcacgccaag tggcaacatc aatcgaccga tggcttcaga agcaaatgct ccgccattca
     1081 taaatggaaa attatttgca cctttttcaa atgacactgc ccattgtgcg ccgcgatata
     1141 agaacaatgt ccaaatcaca ttgaagatca tgaataacac aaaccaagct gcgccagtat
     1201 gtgaaccgta gaaacgacta tctcgcccgt attttgccgg cgaactaaaa actcgaatta
     1261 gggcaaacat tgcaatgcca actaaaacta aaactgcaaa caagtcttct aaaaatccga
     1321 ctaccggtaa tcgaccgatc aatggcacag caaattcagg attaactaaa gcaccgaatg
     1381 cttcaacaat tgttaggcca agaacgataa aaccccagaa tgcaaataca tgtgcgatac
     1441 ctggaactgt ccaagctaaa agtttctttt ggccaagtac ttcggtgact tctgctttaa
     1501 cccgtctctt acgttcggca gttcgaccaa cagctggctg accactacga actaatgagt
     1561 atagaaacca caaccgctta agcgcgaaac caagtaagac aaccgttatg gcaataccga
     1621 cgccgaaacg cagcgccatt gagtcactca ttaatccacc taaaaattct tagtatttgc
     1681 aggttatcgc aatagttaag caaaaaaatg agtagagcct gttatgtaaa tcgcaaatct
     1741 ggctatttat ttttgtgcaa atgttgcatg gcccaggcat acatcgcgat tgcccccgca
     1801 gccgacgcat tcattgatct ggttgaacca agttgagtaa taccaagtat ttcggtgcag
     1861 gcagctaatc cttcttcaga catacctgga ccttcttgcc caaaataaag tacgcagctt
     1921 tctggcagcg aactgtcttc taagtcagca caaccaggaa cattatctat tccgataatc
     1981 ggtacattat tttcaagcga ccacttctta aagtcactta cctctggatg atgaaataaa
     2041 tgtagatagc gatcggtcac catcgcacca cgtctattcc agccacgttt tccgacgatg
     2101 tgcaccccag caacattaaa ggcatttgcg gtacgcacaa tcgaaccaat atttaaatcg
     2161 tgctgccagt tctcaatcgc aatgtgtaat ttgctgcgtg acttatcaag gtctgcaaca
     2221 atagcctcga cagtccaata acgatactta tccacgacat tacgacgatc accaaactca
     2281 agtagttcag gatcaaaatg taaa
//
LOCUS       DBNHVN010000106         2282 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1208342, whole genome shotgun sequence.
ACCESSION   DBNHVN010000106 DBNHVN010000000
VERSION     DBNHVN010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1208342"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="AC16Y1_01940"
     CDS             <1..752
                     /locus_tag="AC16Y1_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018834788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HIR5831258.1"
                     /translation="LTGAQVISADIGLKLDQVDLEVLGTARRIVVSKDNTTIVDGAGD
                     KNAVNDRVGQIKREIENSDSDWDKEKLSERLAKLSGGVCVIKVGAHTEVELKEKKHRI
                     EDAVSATRAAIDEGIVAGGGSAVIHAAEVLKDNLGLTGDQAVGVQIVRRGVIEPLRWI
                     AENAGMEGYVVVDKVRELGVGKGLNAATGEYGDLVAQGVIDPVKVTRSALENAASIAG
                     MLLTTEALVVEKKEPAEDDHGHGHKHAGHTH"
     gene            906..1550
                     /locus_tag="AC16Y1_01945"
     CDS             906..1550
                     /locus_tag="AC16Y1_01945"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012298264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR5831259.1"
                     /translation="MPIDLVIFDCDGVLVDSEQIAIQVDQEVLHNLGWQISIAEIVDR
                     FVGRSNSHFKFEVENYLDIKLPENWSDQLTEKYRERFAAELRPIAGVSEVLAELPYKY
                     CVASSGSIEKMRFTLGHTGLLPKFDGKLFSAEQVIHGKPAPDLFLMAATTMKVKPQNC
                     VVVEDSPAGLQAAAEAGMRAIAYSGGLVSEDKLATFGFPIIWQMVDLPKELAAL"
     gene            1714..>2282
                     /locus_tag="AC16Y1_01950"
     CDS             1714..>2282
                     /locus_tag="AC16Y1_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012669.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR5831260.1"
                     /translation="MLAEERRIQLIEWSQIEGRIDAAVAANRLNVAIETVRRDLDILQ
                     KSGVVRRVHGGAIAVDRIPHEFNLRERRSTNMYAKRRIASVASAQIPSEGAIFVDGGT
                     TTEMLAEFLIDRPKLLVITNSIPLGNLVAESSTPVHLLSGRLRSTTLSALGSRTISDI
                     ATFQTQVVFLGANGISLKAGLTTSDPEEAA"
BASE COUNT          670 a          437 c          546 g          629 t
ORIGIN      
        1 tacttactgg ggctcaggtt atttcagcag atattggttt gaagttagac caggttgacc
       61 ttgaagttct aggtactgct cgtcgcatag ttgtttctaa ggacaacacc acaattgttg
      121 atggtgctgg cgacaagaat gcagttaatg atcgagttgg tcagattaag cgtgaaattg
      181 agaactcgga ctcagactgg gataaagaaa agctgtctga acgcctagcc aaattatcgg
      241 gtggagtttg tgtaatcaaa gttggcgcgc ataccgaagt tgaacttaaa gaaaagaagc
      301 atcgaattga agatgcagtt tctgctactc gcgcagcaat cgatgaaggt atcgttgccg
      361 gtggtggctc tgcagtaatt catgctgctg aagttctcaa agacaactta ggcctaactg
      421 gtgatcaagc agtaggtgtg cagattgttc gccgcggcgt aatcgaacct ttgcgttgga
      481 ttgctgaaaa tgctggcatg gaaggttatg tcgtcgttga caaagtgcgc gaacttggcg
      541 ttggcaaagg cttgaacgca gcaactggcg aatatggcga cttagtagcc caaggtgtta
      601 ttgatccggt aaaggtaact cgaagtgcgc ttgaaaacgc agcttcaatt gctggaatgc
      661 ttttaactac cgaagcatta gttgttgaaa agaaagagcc tgctgaagac gatcacggcc
      721 acggccataa gcatgctggt cacactcatt aattcaaaga attaatgagt gtgactaatg
      781 ggcagagtct aaaactttcc ccgtaatgca ctaattaatt agccaaataa aaacaccaag
      841 caaatttgct gggtgttttt attgtattgc tggacaatca gagactctaa ctgaaaagat
      901 aaagcgtgcc gattgaccta gtcatatttg attgtgatgg tgttctcgtt gactctgagc
      961 aaattgcaat tcaagtagat caagaggtgc tacacaacct tggttggcaa atctcaatcg
     1021 cagaaattgt tgaccgtttc gttggtcgaa gtaatagtca ttttaagttt gaagttgaaa
     1081 actacttaga tattaaattg ccagaaaatt ggagcgacca gctaactgaa aaatatcgag
     1141 aacgatttgc tgccgaatta agaccaattg ctggagttag tgaagtgttg gctgagcttc
     1201 cctataagta ctgtgtggca tcctccggat ctattgaaaa aatgcgtttc acattaggtc
     1261 acacaggttt acttccgaaa tttgatggca aattgtttag cgcggaacaa gtaattcacg
     1321 gaaagcctgc cccagacctg ttcttaatgg ctgcaacgac aatgaaggta aaaccgcaaa
     1381 actgtgtagt cgttgaagac agtccagcgg gtttacaagc tgcagccgaa gccggaatga
     1441 gggctattgc ttatagcggt ggtctagttt ctgaggataa attggcgact tttggcttcc
     1501 caatcatctg gcagatggtt gatttaccaa aagaattagc tgctttataa attttgctcc
     1561 aataaagcct ttaaataagc cactttttaa tccgcaccaa agtcgataac gaaaagataa
     1621 caaatgtgat tatttttctg atttatggtg acttggatca cataactgct aatctccaac
     1681 ataatcaggc aattacgggc agaaagcgac aggatgctgg cagaagagcg cagaattcag
     1741 ttaattgaat ggtctcaaat tgaggggcga atagatgctg ctgttgcagc taatcggctc
     1801 aacgttgcaa ttgaaactgt tcgacgcgat ttagatattt tgcaaaaatc tggagtagtc
     1861 cgaagggtgc atggcggagc tattgcagtt gaccgaatcc cacatgaatt taatcttcgt
     1921 gaacgtcgtt ctaccaacat gtatgcaaag cgacggatcg cttcggtggc ctcagcgcaa
     1981 attccaagtg aaggtgcaat ttttgtagac ggtggaacta ctacagaaat gcttgctgag
     2041 ttcttaattg atagaccaaa actattagtt atcactaata gcatcccact tggaaatctt
     2101 gttgcggaaa gcagcactcc cgttcatttg ctaagtggac gattgaggtc aacaacactt
     2161 agtgcacttg gttcccgaac tatttcagat attgcgactt tccaaactca agttgtcttc
     2221 ttaggtgcaa atggcatttc actcaaagct gggcttacta ctagtgatcc cgaagaagct
     2281 gc
//
LOCUS       DBNHVN010000107         2301 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1950811, whole genome shotgun sequence.
ACCESSION   DBNHVN010000107 DBNHVN010000000
VERSION     DBNHVN010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1950811"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="AC16Y1_01955"
     CDS             <1..690
                     /locus_tag="AC16Y1_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0044877 - protein-containing complex
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HIR5831261.1"
                     /translation="NELPRNVRNELRTLPDGLALRVARHLAASGEMIDIDPELAWQHA
                     RAALNKASRVGIVREAAAEAAYNAGHFAEALTEFRAARRMRGIKEYWPLMADCERALG
                     RPAKAIAMAADPLVNSLDRASKVEMRIVASGARVDQKKLDAALATLQCPELTTTSTEP
                     WAARIRYAYADVLEKLGRKEEALEWFHRAAGVDANEQTDAEERIAALEGVVFEEVEEY
                     EEYEELDEVEN"
     gene            complement(687..890)
                     /locus_tag="AC16Y1_01960"
     CDS             complement(687..890)
                     /locus_tag="AC16Y1_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831262.1"
                     /translation="MDNQEMKFKRNWLEDESISDEEKLAYFDSLDDANVMNIFEKIEA
                     DRQAALAKAAELEKKINERKKLS"
     gene            1026..1520
                     /locus_tag="AC16Y1_01965"
     CDS             1026..1520
                     /locus_tag="AC16Y1_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831263.1"
                     /translation="MTHFWLPDPINPEIDETIHTEYLLRIYEFYERDFLLSKPNSKDY
                     KYSIKVYPDQFGWPATFWHLISEGEPEANRQIRIERASKIVWIRPIINQFFSIYPSGN
                     FENIYWWKNFRNKKISVLLSTLSFDYLVVLEERKNKFLFCTAYPTRPRTAKRLESEFK
                     NYWS"
     gene            1714..2028
                     /locus_tag="AC16Y1_01970"
     CDS             1714..2028
                     /locus_tag="AC16Y1_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR5831264.1"
                     /translation="MWTNSVLLVGRVTGTGEEVELPSGDILTKFRVVVPREKPETKAV
                     VDTIECVSFKPAVQKKIMKLELEDVVEIEGSLRRRFWQGATGVASKMEVEVSSLKHFK
                     QS"
     gene            2025..>2301
                     /locus_tag="AC16Y1_01975"
     CDS             2025..>2301
                     /locus_tag="AC16Y1_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831265.1"
                     /translation="MNIILTEHVMTRMHERDFTIEDIKYVLEAHDFSVPSKKGGTELW
                     RRFPDGRELKVWIAQQIPLPKRVIIKSAGWGGSMSKYKLTVDADASAA"
BASE COUNT          656 a          460 c          553 g          632 t
ORIGIN      
        1 aacgaactcc cgcgaaacgt gcgaaacgaa cttcgcacct tgccagatgg tttggcattg
       61 cgtgtggcca ggcacttggc agcatcgggc gagatgatcg acattgatcc agaacttgcg
      121 tggcaacatg cacgtgctgc attaaataag gcatcacgcg taggtattgt gcgcgaggca
      181 gccgcagaag ccgcttacaa cgctgggcat ttcgctgaag ccttaactga gtttcgtgcg
      241 gcccggcgta tgcgggggat taaagagtat tggccattga tggctgactg tgaacgcgca
      301 ctcggtcgtc cagcaaaagc aattgcaatg gctgccgatc cacttgttaa ttcacttgat
      361 cgagcatcaa aagtggaaat gcgaattgtg gcgtcgggtg ctcgggttga tcaaaagaaa
      421 cttgatgccg cgctagctac tttgcagtgt ccagaattga ccacgacttc gacagaacct
      481 tgggcggcgc gcattcgtta tgcctatgca gacgtattgg aaaaactcgg tcgcaaagaa
      541 gaagcacttg agtggttcca tcgggctgct ggcgttgatg ccaacgaaca gacagatgct
      601 gaagagcgca ttgctgcatt agaaggcgtg gtttttgagg aagtcgaaga gtacgaagag
      661 tacgaagaac tcgacgaagt agagaattaa gagagtttct ttctttcgtt aattttcttc
      721 tcaagttcgg cggctttagc gagcgcagcc tggcggtcag cttcgatctt ttcaaaaata
      781 ttcattacgt tagcgtcatc tagtgagtcg aagtaagcca acttttcttc gtcggagatg
      841 ctttcgtctt ccagccagtt ccgtttgaat ttcatttctt gattatccat caataaaccc
      901 attctcctat gtggataact acaagcggta ttaaggcaaa ttttgataat cctcgttgtt
      961 tgaggaggaa ctaacttttt gaaacttgcc tcgcccgaga gaaattcgat cgatcggatt
     1021 tccacatgac ccacttttgg cttcctgatc caattaatcc tgaaattgac gaaactattc
     1081 acacagaata tttgctcaga atttatgaat tttatgaacg agatttcctt ttatcaaaac
     1141 caaattccaa ggattacaaa tattcaataa aggtatatcc agatcaattt ggctggccag
     1201 cgacattctg gcacctgatt tctgagggtg aacctgaagc aaataggcaa atcagaattg
     1261 aacgggcctc aaaaatagtt tggattagac caattattaa tcaattcttc tcgatttatc
     1321 caagtggcaa ttttgagaat atctattggt ggaaaaattt tcgaaacaaa aaaattagtg
     1381 ttttgcttag cactttaagt tttgactatt tagttgtttt agaggaacgc aaaaataagt
     1441 ttctattctg tacggcatat ccaacaagac ctcgtaccgc caagagacta gaaagtgaat
     1501 ttaagaacta ttggagttaa aaaccgaagc cgcctttcgg cggcttctct gctactccag
     1561 aaactggtta gctagtaata agttagaaca aaaataactc ttagtcaata ccctagacac
     1621 ctttccacag ggtgggtttt tcacaaatag aaaataatcg ctgactcgtt agcttgtcat
     1681 gcgcatactc actcctaatt aataggagga tttatgtgga cgaattcagt cttacttgtt
     1741 ggtcgggtta ccggtactgg cgaagaggtt gagttaccaa gtggcgatat tttgaccaag
     1801 tttcgagttg tggtgccaag ggaaaagcca gaaaccaaag ccgtagtgga caccattgag
     1861 tgcgtgtcat ttaagccggc agttcaaaaa aaaataatga aacttgaact ggaagatgtt
     1921 gttgagatcg aaggctcact tcggcgcagg ttttggcaag gggctaccgg tgtggcttca
     1981 aaaatggaag tcgaggtttc gtcgttaaag cactttaaac aatcttgaac atcattctta
     2041 ccgagcatgt gatgacgaga atgcatgaac gggattttac gattgaagac attaagtatg
     2101 tgctggaagc tcatgatttt tcggtcccta gtaaaaaggg tggaactgaa ttgtggcgcc
     2161 ggtttccaga cggtagagag ttgaaagttt ggattgcaca gcagatccct ttgcccaaac
     2221 gtgtcataat taaatcagct ggatgggggg gatcaatgag taagtacaaa ttaactgtag
     2281 atgccgatgc tagcgctgcc t
//
LOCUS       DBNHVN010000108         3450 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1132980, whole genome shotgun sequence.
ACCESSION   DBNHVN010000108 DBNHVN010000000
VERSION     DBNHVN010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1132980"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /gene="grpE"
                     /locus_tag="AC16Y1_01980"
     CDS             <1..546
                     /gene="grpE"
                     /locus_tag="AC16Y1_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR5831266.1"
                     /translation="DQAVHETEGVKITDKRKLDPQTGEPRSTDQAPVSDEVRLSGSAD
                     PVGELTADLQRLQAEYSNYRKRVERDREVTRELTISGALAEFLPIIDDIGRARTHGEL
                     EGAFKSVGEALESTVHRLGLKPFGQPGDEFDPTRHEAIAHEHSAEVDKSTCVTVYQPG
                     YEFNGKIIRAAIVSVADPVTE"
     gene            586..1545
                     /locus_tag="AC16Y1_01985"
     CDS             586..1545
                     /locus_tag="AC16Y1_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HIR5831267.1"
                     /translation="MAVKDYLEKDYYKVLGVAKDADASEIKKVYRKLAKDLHPDKNPG
                     DKKIEDRFKEVSEAYDVLGDPKRRAEYDEAQMYGGQFRGGFNTNDMFGPGARNFGAGD
                     FGQMGDIGDLGDLLGGFFGGGRPRGPRRGADLETSVTLSFEDAVHGTTLPLRLASHTV
                     QARIPAGVNNDQKIRLKGKGSPGESGAPAGDLFINVTVSPHPVFGRDGNNLTITVPIK
                     FDEAVNGADIKVPVLDGAPVTIRIPAGTKSGSKFRARGKGVAKAQGATGDLIVMVEIA
                     VPEELTEDAKRALNLFASATSEFDPRAQLLEKTGDISKAGERG"
     gene            1517..1885
                     /locus_tag="AC16Y1_01990"
     CDS             1517..1885
                     /locus_tag="AC16Y1_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein transcriptional repressor
                     HspR"
                     /protein_id="HIR5831268.1"
                     /translation="MFRRQASVDNYELSEEIPLYVISVAAQLAGLHPQTLRQYDRLGL
                     VSPDRTLGKNRLYSLKDIGRLREVARLSAEGVTLSGIIRILALEEQLEATLRELAALR
                     AAVQGPSTALVVWRPQRRAL"
     gene            1913..>3450
                     /locus_tag="AC16Y1_01995"
     CDS             1913..>3450
                     /locus_tag="AC16Y1_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HIR5831269.1"
                     /translation="MNENYTTKSQEAIQSAATTATVNGNPTVEAAHILVALLEQTEGI
                     AGALLDTVGADKSAVLKAARDSVEQLPGASGQSVAQPNFSQSAIRAINEAKDLAKARS
                     DEYISTEHLLVGIAKHGGNAADLLKNSGATARALLEAIDTLHGGRRVTNPDPEGTFQA
                     LEKYGNDLTALAREGKLDPVIGRDSEIRRVVQVLSRRTKNNPVLIGEPGVGKTAVVEG
                     LAQRIIAGDVPESLRGKKLIALDLGAMVAGAKYRGEFEERLKAVLEEIKTSEGQVITF
                     IDELHTVVGAGATGDSAMDAGNMLKPMLARGELRLVGATTLNEYREYIEKDAALERRF
                     QQVLVGEPSVEDTVTILRGLKEKYESHHKVNIADSALVAAAALSDRYITSRFLPDKAI
                     DLVDEAASRLRMEIDSSPEEIDQLQRQVDRLRMEELALAKATDEASFERLMKLREDLA
                     NKDEELRTLRARWDQEKAGLDRVGEIKVRIDELRSEAERALRDGDLERASRIQYAELP
                     ELET"
BASE COUNT         1010 a          708 c          891 g          841 t
ORIGIN      
        1 gaccaagcag ttcatgaaac tgaaggcgta aagatcactg ataagcgcaa acttgatccg
       61 caaacaggtg agcctaggtc aactgaccag gcacctgttt cggatgaagt gaggctgtca
      121 ggttcggctg acccagttgg tgagctaact gccgacttac aacgcctgca ggctgagtat
      181 tccaactacc gtaagcgagt tgaacgcgat cgggaagtta cccgcgagct aacgatttct
      241 ggcgcattag ctgagttttt gccgatcatc gatgacatcg gcagagcccg aactcacggt
      301 gaacttgaag gcgccttcaa aagcgtaggt gaagcacttg agtcaacagt tcatcgcttg
      361 ggtttgaaac catttggtca accaggtgat gaatttgatc caactcggca tgaagcgatt
      421 gcgcatgaac atagtgccga agtagataag tcaacctgcg taactgttta tcaaccaggt
      481 tatgaattta acggaaaaat aattcgcgct gcaattgtca gtgtggctga cccggtaact
      541 gaataattca agaagcaagt atcagaaagg aggcgacgaa cgagtatggc tgttaaagat
      601 tatttagaaa aggattatta caaagtactt ggtgttgcca aagatgctga tgctagcgaa
      661 ataaaaaagg tctaccgaaa gttagccaaa gatttacatc cagataaaaa tccgggtgat
      721 aaaaaaatcg aagatcgttt caaggaagtt agtgaagcat acgacgtcct tggtgatccg
      781 aagcgtcgtg ctgaatatga cgaagcacaa atgtatggtg gccaattccg tggtggattc
      841 aataccaacg acatgtttgg tccaggtgct agaaatttcg gcgcaggcga ttttggtcaa
      901 atgggagaca tcggcgacct aggcgattta cttggcggat ttttcggcgg cggaagacct
      961 cgtggtcctc gtcgcggcgc agacctcgaa acttcggtga cacttagctt tgaagatgca
     1021 gtccatggca caacattgcc gttgcgtctg gcatcgcaca ccgtgcaagc tcgtattcca
     1081 gctggagtaa ataacgatca aaaaattagg cttaaaggta aaggcagtcc aggcgaatct
     1141 ggtgcccctg ctggtgactt atttataaat gtaactgtct ccccgcatcc ggtgtttggt
     1201 cgcgatggaa ataacttaac tattaccgtg ccaatcaaat ttgacgaagc agttaatgga
     1261 gcagatataa aagttccagt actagatggc gcgccagtca caatccgaat tccggcggga
     1321 acaaaatcgg gcagtaaatt ccgcgcccgc ggaaaaggtg tcgccaaagc gcaaggagca
     1381 actggtgact taattgtcat ggttgagatt gcggtgccag aagaactaac cgaagatgca
     1441 aagcgcgcgt tgaacttgtt cgctagcgcg acgtcagaat ttgatccacg tgcccagtta
     1501 ctagaaaaga ccggagatat ttcgaaggca ggcgagcgtg gataattacg agttaagtga
     1561 ggaaattcct ttatatgtaa tttcggtagc tgcacagctt gcaggtttgc acccacaaac
     1621 actcagacag tacgaccggt tgggtttagt atcaccggat cgcaccttgg gaaaaaatcg
     1681 actttattca ttaaaagata ttggtaggtt gcgcgaagtt gctcgtttat ctgcagaggg
     1741 cgtgacactt tccggcatta ttcggatttt ggcgcttgaa gaacaacttg aagcaacatt
     1801 gcgtgagtta gctgcattgc gtgctgcagt tcaaggtcca tcaacggcac tagttgtctg
     1861 gcggccacaa agaagggcac tctaaatttc aaaaacactc gggtaggtaa gaatgaacga
     1921 aaactataca actaagtcgc aagaagcaat tcaaagtgca gcaactactg ccacggttaa
     1981 tggaaatcca acagttgaag cagcgcacat acttgtcgca ttacttgaac aaaccgaggg
     2041 tatcgcaggt gcgctgttag atactgttgg cgccgacaag tctgcagtat tgaaagctgc
     2101 tcgcgattcg gtagaacaat taccaggtgc atctggacaa agtgtcgccc aacctaattt
     2161 ttctcaaagt gcaatcagag cgattaatga agctaaagat ttagctaaag cccgcagcga
     2221 tgaatatatt tcaacagagc acctgctagt tggcattgct aaacatggtg gcaatgctgc
     2281 tgatttattg aagaattcag gagcaaccgc tagagcgtta cttgaggcga ttgacacgtt
     2341 gcatggtggc cgccgggtaa ctaatcctga tcctgaaggc actttccaag ctttagaaaa
     2401 atatggcaat gatttaacgg cacttgctcg cgaaggaaaa cttgatccgg tgattggacg
     2461 cgacagtgaa attcgtcgag tggtgcaagt actttctcgg cgtacaaaaa ataacccagt
     2521 gttgattggc gaaccgggtg taggtaaaac agcagttgtt gagggtttag cccagcgcat
     2581 tattgctgga gatgttccag agtcgttacg tgggaaaaaa ttaattgctc tggatttagg
     2641 cgcgatggtt gcgggagcta aatatcgtgg cgaattcgaa gagcgactaa aagccgtttt
     2701 agaagaaatt aaaactagtg aaggccaagt aattactttt attgatgaac ttcataccgt
     2761 agtaggcgca ggtgcaactg gtgattcagc gatggacgct ggaaatatgt tgaaacctat
     2821 gttggctcgt ggtgaactgc gtttagttgg ggcaaccaca cttaatgagt atcgcgaata
     2881 cattgaaaaa gatgcagcac ttgagcgccg gttccaacag gtattggttg gtgaaccaag
     2941 tgttgaagac acggtaacta ttttgcgcgg tctaaaagaa aagtatgaat cgcatcacaa
     3001 agtaaatatt gctgactctg ctttagttgc tgcagctgct ctttctgatc gttacatcac
     3061 atcgcgtttt ttgcctgata aggcaattga tcttgtcgat gaagctgcct cacgcctccg
     3121 gatggaaata gatagttcgc cagaagaaat cgatcaattg caacgtcaag ttgatcgtct
     3181 tcggatggaa gaactagctc tagcaaaagc cacagacgaa gcatcttttg aacggctgat
     3241 gaagttgcgc gaggacttag caaataaaga cgaagaactt cgtactctac gcgcccgatg
     3301 ggatcaagaa aaagctggct tggatcgtgt tggcgaaatc aaagttcgca tcgatgaact
     3361 gcgctcagaa gcagaacgtg cattacgtga tggcgactta gaacgagcaa gtcgtattca
     3421 gtatgccgaa ttgcctgaat tagaaacaga
//
LOCUS       DBNHVN010000109         5446 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1654381, whole genome shotgun sequence.
ACCESSION   DBNHVN010000109 DBNHVN010000000
VERSION     DBNHVN010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1654381"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="AC16Y1_02000"
     CDS             complement(<1..775)
                     /locus_tag="AC16Y1_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831270.1"
                     /translation="MANIISRLLTKKLLTYLVAVATVTITSISGLSPAYAADENAQVR
                     ISNIGVTLDPSTNRYVIRIDGELANNGPTEIRNIRIVVGTENQIVSKYALSRFYDSRD
                     NANLTESNNFAKLRRVSANSKANWNITFFADEVLTFSNGLYGLGVSAYTDARFSSDVV
                     AIPLFTAPPLNVMNTVFAVQLSTLNRHLANGGSYRSDVQEINRLTSLIVNARDLEVTW
                     IVDPVLYQWLAELASTELQEEALNLSNLLAEVSPKSISSV"
     gene            885..2327
                     /locus_tag="AC16Y1_02005"
     CDS             885..2327
                     /locus_tag="AC16Y1_02005"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016472073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIR5831271.1"
                     /translation="MSEQVAPENIASQLIHDALSSMPDVIELGELFKSAGFDLALVGG
                     SVRDLILQRDLGDLDFTTSATPDQIVKITKPWADSVWDLGMEFGTVGVRKNDQVFEIT
                     TFRSEVYVSESRKPSVKFGNDISEDLIRRDFTINAMAVKLPSFEFIDLFGGINDLVKR
                     VIRTPREAELSFSEDPLRMMRVARFASQLEFTPVSDVIIAVTEMRERIRIVSAERVRD
                     EFTKLIMGANPLHGLEFLIGTNLADEIIPELPALQLEIDEHHKHKDVYEHSLKVLSQA
                     IELEALHEPTCEPDLVLRLAALLHDIGKPKTRKFESGGGVSFHHHEVVGAKIARKRLV
                     AMRYPNEVIDGVTKLIELHLRFHGYGDGQWTDSAVRRYVRDAGDELVRLHKLTRADCT
                     TRNQRRAMMLAATYDDLEDRIAKLQEQEELNAIRPDLDGEEIMTALGLKPGPQVGKAY
                     NHMLNVRLDKGPLGKDEALVELKNWWASQN"
     gene            complement(2324..3670)
                     /locus_tag="AC16Y1_02010"
     CDS             complement(2324..3670)
                     /locus_tag="AC16Y1_02010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5831272.1"
                     /translation="MRKRSRNSLSIFKGENSRRLLFVRIFGQAGDGLLQTALATFILF
                     SPQRAVNPEQIAVAFAILLIPYSIVGPFVGLFIDSWSRRSILFRANILRVFTMVAVAL
                     VVLGHQANQLLAILVLLSLGINRFIQAALASSIPHVVDETNLVGANALFPTLGTVAAT
                     IAVVAGIVTQQIFGNTDEVNAGLIAFGALLALVAAYFATKFTPVDVLGPHGVTLEIRR
                     EFRNVLTGLLAGFNRLRRAPNAKLSISSVALQRTVFGTLTVTALLLARNIWNDPSNPD
                     AAISDFGYAAISAGAGSFVAAIVAASLMNGRIERITKSMDERQSVLILISTFATGAAL
                     VVTVAGFMSQTITGILLTAFTLGLAGQFLKINADTTIQRDIDDAHRGRVFSIFDMSIN
                     VSIVIGICIFTLFDVVRDELVLRITIAATGLLAVVMITGYHYFKSRNLLKLDQQQN"
     gene            complement(3792..4865)
                     /locus_tag="AC16Y1_02015"
     CDS             complement(3792..4865)
                     /locus_tag="AC16Y1_02015"
                     /EC_number="5.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019356732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HIR5831273.1"
                     /translation="MSSVRVAIVGVGNCASSLVQGVEYYKDADPNKQVPGLMHVQFGD
                     YHVRDVEFVAAFDVDAKKVGQDLAHAIFASENNTIKFSEVPPTGVTVQRGNTLDGLGK
                     YYREMVIESDDKPVDVVKALKDAKVDVLVCYLPVGSEQAAKFYAQCAIDAGVGFVNAL
                     PVFIAGTKEWADKFTAAGVPIVGDDIKSQVGATITHRVLAKLFEDRGVIVDRTYQLNV
                     GGNMDFMNMLERDRLESKKISKTQAVTSQLQHDLGARNVHIGPSDYVAWLDDRKWAFV
                     RLEGRAFGDVPLSLEYKLEVWDSPNSAGVIIDAIRACKIALDRGIGGPILSASSYFMK
                     SPPIQYDDTTCRNLVEDFIAGKA"
     gene            complement(4862..>5446)
                     /locus_tag="AC16Y1_02020"
     CDS             complement(4862..>5446)
                     /locus_tag="AC16Y1_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018553898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR5831274.1"
                     /translation="ELSVLGILSTSPLHGYELRKRLISVTGLFKTISYGSLYPILKSL
                     VERGFLAEQDDNPSNKYPKRNRIVYVITDEGKKHLNSLLKDSGPAAWDDDNFAVRMSL
                     FAQTSADTRIHILQGRRSRLEIRLADLRTNLAKGRERLDAYTLELQQHGLDALEREVR
                     WLNELISHEQSGGLKKSTNSNAKNITAANKEAMQ"
BASE COUNT         1636 a         1159 c         1130 g         1521 t
ORIGIN      
        1 agacactcga aatagatttt ggcgaaactt cagccagcaa gtttgaaaga ttaagcgctt
       61 cttcttgtag ttcagtacta gctaactccg ctagccattg atacaaaacc ggatccacaa
      121 tccaagtaac ttccaaatcg cgggcgttaa caattaatga agtcaatcga ttgatttcct
      181 gcacatctga gcgataagaa ccaccgttag ctaaatgcct atttagcgtt gaaagctgaa
      241 ccgcaaatac tgtgttcatt acatttagtg gcggggcagt aaacaacgga attgctacaa
      301 catctgagct gaatcttgca tcggtatatg cgctaactcc taaaccgtat aaaccatttg
      361 aaaaagttag aacttcatcc gcaaaaaaag tgatattcca atttgctttg ctattcgcag
      421 acactcttcg aagttttgca aaattatttg attcagtcag gtttgcatta tcccgactgt
      481 cataaaaacg agaaagtgca tatttactaa cgatttggtt ttcagtgcca acaactattc
      541 tgatatttct gatttcagtt ggaccattgt tagctagttc gccatctatt cgaattacat
      601 atctatttgt gcttggatct aatgtcacac caatgtttga aattcgaact tgagcatttt
      661 catcagcggc ataagctgga cttagacctg aaattgaagt gatagtaact gtggcaacag
      721 caactaaata agttaataac ttcttagtta gtagcctgct aataatattt gccacaaata
      781 cagaatactt ggcttaattg acccttattt ttaaacctta ttcactgata gtacatttca
      841 atgtttactc accttttatt aaagcaaata ggctataact cgtaatgagt gagcaagtcg
      901 ccccagaaaa catcgcatca caattaattc atgatgcgct atcaagcatg cctgacgtga
      961 ttgagttagg tgaattattt aaaagcgctg gttttgattt agctttagtt ggcggaagtg
     1021 ttcgcgacct aattttgcaa cgagacttag gtgatctaga tttcaccacg tcagcaactc
     1081 ctgaccaaat tgtgaaaatc actaaaccgt gggctgattc ggtttgggat ttaggaatgg
     1141 agtttggcac agttggggtg cgcaaaaacg atcaggtttt tgaaatcaca acttttcgaa
     1201 gcgaagtcta tgtatcagaa agtcgaaagc cttcggttaa attcggcaac gacataagtg
     1261 aagacttaat tcgccgcgat ttcacaatta atgccatggc cgtaaagttg ccatcttttg
     1321 agtttattga tttatttggc ggaattaatg acttagttaa acgagttatt cgaacgccgc
     1381 gggaagccga actttcattt tctgaggatc cgcttcgcat gatgcgggta gcccgttttg
     1441 ccagccaact tgaattcacg ccagtttctg acgtaattat cgctgtaacg gaaatgcgtg
     1501 agcgtattcg aattgtttca gctgaacgtg tacgcgatga gttcaccaaa ctaataatgg
     1561 gtgccaatcc attacacggt ttagaatttt taattggaac taatcttgca gatgaaataa
     1621 ttcctgagct tccggcttta caacttgaaa tcgatgagca tcacaaacat aaagatgttt
     1681 atgaacatag tttgaaagta ctaagtcaag cgattgagtt agaagcatta catgaaccca
     1741 cttgtgaacc cgacctagtt ttgcgcttgg ctgctttatt gcatgacatc ggcaaaccaa
     1801 aaactcgaaa gtttgaatca ggtggtggag tcagttttca tcatcatgaa gttgtgggcg
     1861 caaaaattgc gcgcaaacgt ttggttgcga tgcgatatcc aaacgaggtt atcgatggcg
     1921 tgacaaagct catcgagctg catttgcgtt ttcacggcta tggtgatggt cagtggactg
     1981 actctgctgt gcgcagatat gtacgcgatg ccggtgatga attagttaga ttacataaat
     2041 taactcgggc tgattgcacc actcgcaatc aacgacgagc catgatgttg gcggcaactt
     2101 atgacgacct agaggatcgg attgcaaaac ttcaagaaca agaagaactc aacgcaatcc
     2161 gacctgattt agatggtgaa gaaattatga ctgcgctggg ccttaaacca ggtccacaag
     2221 ttggtaaggc ctataaccat atgcttaatg tgcgcctaga taaaggtcct cttggtaaag
     2281 acgaagcact cgtcgaatta aaaaattggt gggcaagcca aaactaattt tgctgctgat
     2341 caagttttag taaattccga gatttgaaat agtgatatcc ggtaatcatc accacagcaa
     2401 gtaaacctgt agcggcaatt gttattcgaa gtaccaattc atctctgacc acatcaaaca
     2461 aagtaaaaat acaaatgcca ataacgattg aaacatttat tgacatatca aaaatagaaa
     2521 aaactcggcc acgatgtgca tcatctatat cgcgttgaat agtagtgtcg gcgtttatct
     2581 ttaaaaactg tccggctaaa cctaaagtga acgcggttag caaaatacca gtaattgttt
     2641 gactcataaa tccagcaacg gtaacaacca gcgcagcccc agtagcaaaa gttgaaatta
     2701 aaattaaaac cgattgacgt tcatccattg attttgtgat tcgttcaatg cgaccattca
     2761 taagtgatgc ggccactatg gcagcaacaa atgatccagc accggcagaa attgctgcat
     2821 agccaaagtc tgaaatcgcc gcatctggat tactcggatc attccaaata ttgcgggcaa
     2881 gtaataacgc ggtaacagtt agtgttccaa aaacagttcg ttgcagagct acagaactaa
     2941 tacttaactt ggcattcggt gcgcgtcgca aacgattaaa cccagcaagt aatccagtta
     3001 gtacatttcg aaattcgcgc cttatttcta atgtgacgcc atgtggccct agtacatcga
     3061 ccggggtgaa ttttgtggca aagtaagcgg caactaaggc taagagtgcg ccaaacgcaa
     3121 tcaagccagc atttacttca tcggtgtttc caaatatttg ttgcgttact attccagcta
     3181 ccactgcaat ggttgcggca actgtgccca aagtcggaaa tagcgcatta gctccaacca
     3241 gatttgtttc atcaactacg tgtggaattg atgaagcgag agcagcctgg ataaatcgat
     3301 taatacctaa actcaacaaa accaaaattg ctaaaagttg attagcttga tgacctagaa
     3361 caactagggc cacagcaacc atggtgaaaa cacgcaaaat atttgctcta aacaaaatag
     3421 agcgtctgga ccagctgtcg ataaaaagcc caacgaacgg cccgacaatt gaatagggaa
     3481 ttaataaaat cgcaaacgca actgcaattt gttcaggatt aaccgcgcgt tgtggcgaga
     3541 acaaaataaa tgttgccaag gctgtttgaa gtaagccatc accagcttgg ccaaaaatgc
     3601 gaacaaataa aagtcggcga gaattttcgc ccttgaaaat acttagcgaa tttcgacttc
     3661 gcttacgcat cacattcaca ataaaacgtt acctgtaatc aaaatttaag ttcgttggac
     3721 atcatgtttt aggaaaaaag aaggacccac tttcgtgggc ccttcttcag gaggctagat
     3781 gtttagtttg attacgcctt gccagcaatg aagtcttcta ccaaattgcg gcacgttgtg
     3841 tcgtcatatt ggataggcgg actcttcata aagtaactcg aagctgaaag aattgggcca
     3901 ccaattccac gatccaaggc aattttgcat gcgcggattg catcgatgat tacaccagca
     3961 gagttagggg aatcccacac ttcaagcttg tactcaagac tcaatggcac atcaccaaat
     4021 gcacgacctt caagtcgaac aaatgcccac tttcgatcgt ctaaccaagc aacatagtca
     4081 gatggaccga tgtgtacgtt acgcgcacca aggtcatgtt gtaactgtga agttactgct
     4141 tgagtttttg aaatcttttt agattcaaga cgatcacgtt ccaacatgtt catgaagtcc
     4201 atgtttccac caacatttaa ttggtaggtg cggtcaacaa ttacgccacg atcttcaaat
     4261 agtttggcta gaacgcggtg ggtgattgtg gcaccaacct gactcttaat gtcgtcacca
     4321 acaattggaa cacctgctgc agtgaacttg tcggcccatt cttttgtgcc tgcaataaat
     4381 accggaagcg cattaacgaa accaacacct gcatcgattg cacattgagc gtagaacttt
     4441 gctgcttgct cagacccaac tggtaagtag caaactaaga catccacttt ggcatcttta
     4501 agtgctttaa ccacatctac tggtttgtca tctgattcga taaccatttc gcggtaatac
     4561 ttaccaaggc catcgagtgt gttaccgcgt tgcacagtaa ctccagtcgg tggaacttca
     4621 gagaatttga tggtgttatt ttcgctggcg aagattgcat gtgctaaatc ttgaccaact
     4681 tttttggcgt ccacatcaaa tgcagcgaca aattcaacgt cgcgcacgtg gtagtcgcca
     4741 aactgaacgt gcatcagacc tggtacttgc ttatttggat cagcatcttt gtagtactca
     4801 actccttgca caagtgatga agcgcagtta cctacgccaa ctattgctac ccttactgaa
     4861 ctcactgcat agcctccttg tttgctgcgg tgatattttt tgcatttgag ttagttgact
     4921 tttttaagcc acctgactgt tcatgcgaaa tcaactcgtt taaccaacgc acttcacgtt
     4981 caagtgcgtc gagtccatgt tgttgaagtt ctaatgtgta agcgtcaagt cgttctcgac
     5041 ctttagctaa atttgtgcgc aagtctgcaa gtcttatttc aagtcgactc cggcgacctt
     5101 gcaaaatatg tattcgagtg tctgctgagg tttgcgcgaa tagcgacatg cgcaccgcaa
     5161 aattgtcatc atcccacgca gctggaccac tatcttttag cagagaattt aagtgttttt
     5221 taccttcatc agtaattaca taaacaatgc gattacgttt cggatattta tttgacggat
     5281 tatcgtcttg ttctgccaaa aagccgcgct cgactaaaga cttgagaatt gggtaaagcg
     5341 atccgtatga aatagtttta aataaaccgg tgactgagat taatcttttg cgaagttcgt
     5401 agccatgcag cggactagtt gaaagaatgc caagcaccga aagctc
//
LOCUS       DBNHVN010000110         2137 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1209021, whole genome shotgun sequence.
ACCESSION   DBNHVN010000110 DBNHVN010000000
VERSION     DBNHVN010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1209021"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="AC16Y1_02025"
     CDS             complement(<1..740)
                     /locus_tag="AC16Y1_02025"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase
                     family protein"
                     /protein_id="HIR5831275.1"
                     /translation="MTDLISENLDVSAAQVVASGAKLYPAKTRISSSDKIVRNPTKNP
                     ASKEARDAILAAPGFGKYFSDNIVRAVWLKDKGWQQAELIGQDASAGGLAINALHYGQ
                     SIFEGLKAYRHPDGGIYTFRPEANALRFQRSAYRLALPPVPTDLFIGAIEALVAQDQD
                     WVSQDPEKSFYLRPLEFGSEPALGVKPSDEVTFVLMGSPVGAYFPNGVKPVKVWISDD
                     FVRAAPGGTGEAKCAGNYAASLLAHSEAA"
     gene            complement(759..1802)
                     /locus_tag="AC16Y1_02030"
     CDS             complement(759..1802)
                     /locus_tag="AC16Y1_02030"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR5831276.1"
                     /translation="MAKDFKLAVIAGDGIGTEVVEQGLKVLSAVAPTAGFNFSTSEYD
                     LGARRYNTTGETLPDSVVEELKKHDAILLGAIGDPSVAPGILERGVLLPLRFLLDHHV
                     NLRPVKLFPGVSSPIAGKGPKEIDFVVCREGTEGPYVGAGGFLRKNTIHEVATEVSIN
                     TRFGAERIIRDAFNRATKRRNKVTLVHKTNVLVYAGDVWARTFAEVAKEFPQVETDYC
                     HVDAASMFFLTNPERFDIVVTDNLFGDILTDIGAAIAGGIGLAASGNIDPSRTNPSMF
                     EPVHGSAPDIAGQGIADPTATILSVVMLLDHLGHIDAAKKVEAAVAADLASRTGTRST
                     NQIGDAIASAVAN"
BASE COUNT          596 a          519 c          441 g          581 t
ORIGIN      
        1 gcagcttctg agtgggctaa caaacttgcg gcatagtttc cagcacattt tgcttcacct
       61 gtgccaccag gagcggctcg gacaaagtca tccgagatcc aaacttttac cggcttaacc
      121 ccatttggaa aataagcgcc aactggtgag cccattaaaa caaaagtcac ttcgtccgaa
      181 ggtttcaccc caagggctgg ttcactacca aattcaagtg gtctcaagta gaacgacttt
      241 tctggatctt gtgaaaccca atcttgatct tgcgcaacca gagcttcgat tgctccaata
      301 aataagtcag tcggtaccgg tggaagtgct aaccgatatg cggatcgttg aaaacgaagt
      361 gcatttgctt ctggacggaa tgtgtagatt ccaccatcgg gatgacgata tgccttcaag
      421 ccttcgaaaa ttgattgtcc ataatgcaac gcattaattg ccaacccacc tgcactggca
      481 tcttgaccga ttaattcagc ttgttgccaa cccttatctt ttaaccagac tgcgcgcact
      541 atgttgtcag aaaaatactt tccaaaacct ggggcagcca aaattgcatc tcgtgcttct
      601 ttgctggctg gattcttggt tggattgcgc acaattttgt cgctgctgct aattcgagtt
      661 ttagcgggat aaagttttgc acctgatgca acaacttgag cagcagaaac atctaagttt
      721 tcactgatta aatcagtcat gctacttcgc ccttcgtttt agttagcaac cgcagatgca
      781 attgcatcgc cgatttgatt ggttgatcta gttccagttc tacttgctaa gtccgcagct
      841 accgcagctt caactttctt tgctgcatca atatgaccca aatgatcaag cagcatcaca
      901 acactcaaaa ttgttgcagt cggatctgcg atgccttgcc ccgctatgtc aggtgcactg
      961 ccatgaactg gctcaaacat acttgggttt gttcgactcg gatctatgtt gccactagcg
     1021 gcaagtccga taccgccggc tattgccgcg ccgatatcag tcaggatgtc accaaataaa
     1081 ttgtccgtta caactatgtc aaagcgttca ggattagtta agaagaacat gctggctgca
     1141 tcgacgtgac aataatcagt ttcaacttgt ggaaattctt tagcaacctc ggcaaaagtt
     1201 ctagcccaaa cgtcaccggc gtaaactaaa acattagttt tatgaaccaa agtgacttta
     1261 ttgcgtcgtt tcgtagcacg attaaaggca tctcgaataa ttcgctcagc accaaaccta
     1321 gtattaatac tgacttcagt tgctacttcg tgaatcgtat ttttgcgcaa aaatccgccc
     1381 gctcccacat aagggccttc agttccttcg cggcacacca cgaaatcaat ttctttgggt
     1441 cccttaccgg cgattggtga actaactccg gggaaaagtt tcactggacg cagatttaca
     1501 tggtgatcaa gtaaaaatct aagcggtaat aaaacacctc gctctaaaat tcctggcgct
     1561 accgaaggat caccgatcgc acccagcaaa attgcatcat gttttttcag ttcttcaaca
     1621 actgaatcgg gcaaagtttc tccggttgtg ttgtagcgcc gagcgccaag atcatattcc
     1681 gaagttgaaa aattaaagcc agctgttggg gctacggcag acaaaacttt tagaccctgc
     1741 tcaacgactt cagtgccaat accatcgcca gcgattaccg ctaatttaaa gtcttttgcc
     1801 atggataaag ggtatcgggg taaggctcaa ttaaaaaaat taagtattgc agatctaaac
     1861 ccgttcaaag ttgacggcca ctttcgattt aacaaaccgt aattattgaa ggtaggcttt
     1921 aggcaatgta cgtgagcctg ctacttcgca gccgcaacga ggcctaataa tccggccttc
     1981 tcgttgcggt gtttgtgctt gctggatagc caaagtgtca aaaacttaat aattcgtaca
     2041 taaccactgg agactttcaa atgaataaat atgaagacca taatcggcaa aaaccatcgg
     2101 gtatgccatt taatcgctac gaaccgttta acccaat
//
LOCUS       DBNHVN010000111         1687 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_243276, whole genome shotgun sequence.
ACCESSION   DBNHVN010000111 DBNHVN010000000
VERSION     DBNHVN010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_243276"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="AC16Y1_02035"
     CDS             complement(<1..232)
                     /locus_tag="AC16Y1_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5831277.1"
                     /translation="MTQQPLLAVNNLGVEFQTEDGVVHAVTGVSYEVYRGKTLAIVGE
                     SGSGKSVSSLAVMDLLGRSATVTGSVKFDGREL"
     gene            complement(232..1218)
                     /locus_tag="AC16Y1_02040"
     CDS             complement(232..1218)
                     /locus_tag="AC16Y1_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006608094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5831278.1"
                     /translation="MFKYIIRRTGLMIVMLGILSVITFLLFNAVPTDPAALTCGKSCS
                     PAIIEANRIRLGLDQPLYVQYIEWIRGIFFGRTYGSGSQTFQCDVPCLGYSFRNGEEV
                     TDLIVNALPVTFYLAIGAFTLWMLVGITSGIYAAKKRGKWQDRAIMGTTLVGYSLPVF
                     FVGLLLLIFIVVRWNFLPYPDYEPPTKDPAAFFQTMFLPWVALAILYAAYYTRLTRSQ
                     MLETLGEDFIRTARAKGLPERTVVRKHAFRAGLTPIVTSAGLDLAGLLGGAVITEYIF
                     SLPGLGRVAIGAVTEYDLPTITAVTMVAAVFVIIANLVVDILYKAIDPRVRL"
     gene            complement(1314..>1687)
                     /locus_tag="AC16Y1_02045"
     CDS             complement(1314..>1687)
                     /locus_tag="AC16Y1_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5831279.1"
                     /translation="GYYGVVFQEDLQGGLTSGGWAPDWTNASTVIPELYTPSGGFALA
                     RYDDAAWNERVAAAKAMTDRAAQAKEWQALNTEAMKKALILPTRFGLEQRIHGSKVTG
                     AYIWGPYGSWPYASLGVEAAN"
BASE COUNT          490 a          404 c          369 g          424 t
ORIGIN      
        1 ttagttcgcg gccatcaaat ttaacgctgc cagttactgt cgctgagcgg ccaagtaaat
       61 ccattaccgc caagctagag acacttttac cagaacctga ctcgcctact atggctaaag
      121 ttttcccgcg gtagacctcg taacttacgc cggttactgc gtggactacc ccatcttcag
      181 tctgaaattc aacacctaaa ttattaacgg caagtaatgg ttgctgtgtc attacaacct
      241 cactcgtgga tcgatggctt tgtacaaaat atcaacaact aaattagcga taattacgaa
      301 caccgctgcg accatagtta ccgcagtaat tgttggcagg tcatattcag tgactgcgcc
      361 gattgcaacg cgacctaaac ctggcaaact gaaaatatat tcagtaataa ctgcgccgcc
      421 taatagtcca gctaaatcta agccagctga agtgacaatc ggtgttaagc cggcacgaaa
      481 tgcgtgtttg cgaacgacag tgcgttctgg caatccctta gcgcgcgcgg tccgaatgaa
      541 atcttcgcct agtgtttcta gcatttggct tctagttaag cgtgtgtaat aagccgcgta
      601 cagaatcgct agcgcaaccc aaggcaaaaa catggtttga aaaaatgctg ctggatcttt
      661 agtcggcggt tcatagtctg gataaggcaa aaagttccag cgaaccacaa taaaaatcaa
      721 aagtagtaag ccaacaaaaa atactggtaa cgagtatcca acaagtgtgg ttcccataat
      781 tgcgcgatct tgccactttc cgcgcttctt ggctgcataa atacctgagg taattccaac
      841 tagcatccaa agtgtgaatg caccgatggc taaataaaaa gttactggta atgcgttaac
      901 aattaagtca gttacttctt ctccgttgcg gaatgaataa cctagacaag gtacatcgca
      961 ctgaaatgtt tgtgagcctg aaccatatgt gcgaccaaag aaaattccgc gaatccattc
     1021 gatgtactga acgtagagcg gctgatctag ccccaggcga attcgattag cttcgataat
     1081 tgcgggtgaa catgacttgc cacaagtcaa ggctgcggga tcagttggta cggcattaaa
     1141 aagtaaaaat gtaatcacgc tcaaaatacc gagcatgaca atcatcaatc cggtgcgtcg
     1201 aattatgtac ttaaacacgc aacctcgcta acgcccaact tgaaaattta ttaagtaaaa
     1261 agttttattt gatagatgtg ggcggagttt cctccgccca catcttttta cagttagtta
     1321 gcggcttcta caccaaggga agcatatggc catgaaccat aaggacccca aatgtaagcg
     1381 ccagtaacct ttgaaccgtg gatacgctgt tcaagtccaa agcgggtcgg cagaatcaga
     1441 gctttcttca tagcttctgt attcagtgcc tgccattctt tagcctgtgc agcgcgatca
     1501 gtcattgcct tagcagcagc gacacgttcg ttccaagcag catcgtcgta acgagctagc
     1561 gcgaaaccac ctgatggtgt gtacagttca ggaataacgg ttgaggcgtt tgtccagtct
     1621 ggagcccaac cacctgaggt taatccacct tgtagatcct cttggaagac tacgccgtag
     1681 taaccac
//
LOCUS       DBNHVN010000112         2432 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_911186, whole genome shotgun sequence.
ACCESSION   DBNHVN010000112 DBNHVN010000000
VERSION     DBNHVN010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_911186"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..767
                     /locus_tag="AC16Y1_02050"
     CDS             36..767
                     /locus_tag="AC16Y1_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024248.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5831280.1"
                     /translation="MFASLTSPKDAASRDIRNNKVVKIDSSAAGNVGRALLAAGSATA
                     ADLADELGLTGAGIRKHLDALLAAGLVQASERASYGPAALAGPKGRGRPAKVFMLTSK
                     GRKIFGEREESLALTALKFMATYSGDQAIDKFAEQIAQNFAQTHTDVSKFKTPQERAM
                     ALVDALNTEGFAATLTPGLGDSVQICQHNCPMGDVAEQFPQVCEAETEMFSQLTGVHV
                     TRLATIATGNPICTTLVPNPRRDQK"
     gene            764..2173
                     /gene="sufB"
                     /locus_tag="AC16Y1_02055"
     CDS             764..2173
                     /gene="sufB"
                     /locus_tag="AC16Y1_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIR5831281.1"
                     /translation="MTTQQEHLDALGSYEYGWHDKNDAGANAKRGLSEDVVRDISAKK
                     NEPQWMLDLRLKGLGLFGKKPMPNWGSDLSGIDFDNIKYFVRSTEKQATSWEDLPDDI
                     KNTYDRLGIPEAEKQRLVAGVAAQYESEVVYHKIREDLEEQGVIFLDTDTALREHEDV
                     FKEYFGTVIPVGDNKFAALNTAVWSGGSFIYVPKGVQVEIPLQAYFRINTENMGQFER
                     TLIIVDEGAYVHYVEGCTAPIYSSDSLHSAVVEIIVKKNARCRYTTIQNWSNNVYNLV
                     TKRAVAHEGATMEWVDGNIGSKVTMKYPAIWLQGPHAKGETLSIAFAGEGQHQDAGAK
                     MVHAAPYTSSSIISKSVARGGGRTSYRGLVQVLEGCHGSKSTVKCDALLVDDISRSDT
                     YPYVDVREDDVALGHEASVSKVSEDQLFYLMSRGLDEDEAMAMIVRGFVEPIARELPM
                     EYALELNRLIELQMEGAVG"
     gene            2176..>2432
                     /locus_tag="AC16Y1_02060"
     CDS             2176..>2432
                     /locus_tag="AC16Y1_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831282.1"
                     /translation="MSIALDHAPSKFAFTANEFPEITGREEEWRFTPIKRTKGLVSGE
                     AKFDGDITVTAEQLGEAKFELVTKNQNRKGYTDRVAALGFE"
BASE COUNT          694 a          522 c          601 g          615 t
ORIGIN      
        1 ggatcttttt caggtttcga gtgaggaaat tgctcatgtt tgcaagccta acgtccccta
       61 aagatgcggc ttctcgggat atacgcaaca ataaggttgt gaaaatagat agttcagcag
      121 ctggcaacgt cggtcgtgct ttacttgcgg ctggctctgc aaccgctgct gatttggcag
      181 atgaacttgg tttaactggc gcaggcatcc gaaaacattt agatgcactc ttagcagctg
      241 gattagttca agcaagtgag cgtgcctcgt atggacctgc tgcacttgct ggacctaaag
      301 gtcgtggacg accagccaaa gtttttatgt tgacttcaaa aggtcgcaaa atttttggtg
      361 agcgtgagga aagtttggca ttaactgcac ttaagttcat ggccacttat tcaggcgatc
      421 aggccatcga taaatttgca gaacagatcg cgcagaactt tgctcaaact catactgatg
      481 tatcaaaatt taaaacccca caagagcgcg cgatggcctt agttgatgca cttaacaccg
      541 aaggtttcgc cgccacctta acgccaggtt taggcgactc agttcaaatt tgtcagcaca
      601 actgtccgat gggcgatgtt gctgaacaat ttccgcaagt ctgtgaagct gaaacagaaa
      661 tgttttcgca actcaccgga gtgcacgtta ctcgactggc aacaattgcc acaggtaacc
      721 caatctgtac cacgctagtt ccaaacccac gaagggatca gaaatgacaa cacaacaaga
      781 gcacttagat gccctaggta gctatgagta cgggtggcat gacaagaatg atgctggggc
      841 aaatgcaaaa cgtgggttga gtgaagatgt cgtgcgcgat atctcggcca agaaaaatga
      901 gccgcagtgg atgttagatc tgcgccttaa aggtttaggg ctattcggta aaaaaccaat
      961 gccgaactgg ggaagcgacc taagtggaat tgatttcgac aacatcaagt acttcgtacg
     1021 ttccacagaa aagcaagcaa ccagttggga agaccttcct gatgacatca aaaatactta
     1081 tgaccgctta ggtatcccag aagctgagaa acaacgttta gttgctggtg ttgcagcgca
     1141 atatgaatct gaagttgttt accacaagat tcgtgaagac cttgaagagc aaggtgtaat
     1201 tttccttgat acagatacag cacttcggga acacgaagat gtgtttaagg aatattttgg
     1261 caccgtaatt ccagtaggtg acaacaagtt tgctgcactg aatactgctg tttggtctgg
     1321 tggttcattt atctatgtac caaaaggtgt gcaggtagaa attccacttc aggcatactt
     1381 ccggatcaac accgaaaaca tgggtcagtt cgaacgaact ctgatcattg ttgatgaagg
     1441 agcttacgta cattacgtcg aaggctgtac tgcaccgatt tattcttctg actcacttca
     1501 ttctgcagtg gttgaaatta tcgtgaagaa aaatgctcgt tgccgttaca ccaccattca
     1561 aaactggtcg aataacgtct acaacttggt aacaaagcgt gcagtagcac atgaaggtgc
     1621 aaccatggaa tgggtcgatg gaaacatcgg ttctaaagta accatgaagt atccagcgat
     1681 ctggcttcag gggccacacg ctaagggcga aacgctttcc atcgcatttg ctggggaagg
     1741 tcaacaccaa gatgcaggag caaaaatggt gcacgcagca ccgtacacat cttcgtcaat
     1801 catcagtaag tcagtcgctc gtggtggcgg acgcacttca taccgcggtc tggtccaggt
     1861 actggaaggc tgccatggtt caaagagcac tgttaaatgc gatgccttgc tagttgatga
     1921 catatcgcgc tcggatacct atccgtatgt ggatgttcgt gaagacgatg ttgcgctagg
     1981 acacgaagct tcagtttcga aagtttctga agatcagttg ttctatttaa tgagtcgcgg
     2041 acttgatgaa gatgaagcta tggccatgat tgtgcgcggt ttcgtcgagc cgattgctcg
     2101 cgaacttcca atggaatatg cactggaact aaaccgacta attgaacttc aaatggaagg
     2161 tgcggtcggc taaggatgtc tattgcgcta gatcatgccc cgagtaaatt tgcttttacg
     2221 gcaaacgaat ttcccgaaat aaccggacgt gaagaagaat ggcgttttac gccaatcaaa
     2281 cgcactaaag gtttagtttc aggcgaagca aagtttgatg gcgacattac tgttactgcc
     2341 gaacaattgg gcgaagctaa atttgagtta gttaccaaga atcaaaaccg aaaaggttac
     2401 accgaccgtg tagctgccct tggttttgaa aa
//
LOCUS       DBNHVN010000113         9612 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1802926, whole genome shotgun sequence.
ACCESSION   DBNHVN010000113 DBNHVN010000000
VERSION     DBNHVN010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9612
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1802926"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="AC16Y1_02065"
     CDS             complement(<1..311)
                     /locus_tag="AC16Y1_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831283.1"
                     /translation="MSSQTPLRPQQRPAPQRVQRPVPQPFQQQRFYEPRPPAVFQLAS
                     PRRRVIRFTQVLAGIFIIYAGQLVNLQIIRGPELAAKSQSNRLQTVLLPAIRGNINDV
                     NG"
     gene            complement(308..796)
                     /gene="ftsL"
                     /locus_tag="AC16Y1_02070"
     CDS             complement(308..796)
                     /gene="ftsL"
                     /locus_tag="AC16Y1_02070"
                     /inference="COORDINATES: protein motif:HMM:NF053549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HIR5831284.1"
                     /translation="MSAMPARAPRLNPIPTRRKRVSRLVAVNSRAIVTPIANAIPVGI
                     PKGRANRNTFVGIIFGMLVFGSFLLLIINTQAQQAAFEKLALQIELNQMLATEQRLAS
                     EVAAAESTDNLVTAARTIGMVPAETPVFLRLSDRVIVGQPVPAKEPAVPFVPPTPAPV
                     VP"
     gene            complement(793..1740)
                     /gene="rsmH"
                     /locus_tag="AC16Y1_02075"
     CDS             complement(793..1740)
                     /gene="rsmH"
                     /locus_tag="AC16Y1_02075"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009188116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR5831285.1"
                     /translation="MTTYSHIPVFKQRCLDLLAPALKQDGAVLVDATLGLGGHTESAL
                     ETFPNLRVIGLDRDKDALTHSQVRLAAFAGRVEFIHAVYDELPEVLAQLGMPTVQGIL
                     FDLGVSSMQLDRAERGFSYSQSAPLDMRMNPTEGQTAADVVNTYSHGELARILSTYGE
                     ERFASRIASAIVKERENQLFTTSDRLVTVVRDAIPAATRRAGGNPAKRTFQALRIEVN
                     DELTVLSRAIPAAIEALAIGGRLVVLAYQSLEDRIVKSAFREVTQSKIPVDLPFIPAG
                     QKPKLQLLTKSAELATEEEIAQNPRAASVRLRAIERLAA"
     gene            complement(1859..2278)
                     /gene="mraZ"
                     /locus_tag="AC16Y1_02080"
     CDS             complement(1859..2278)
                     /gene="mraZ"
                     /locus_tag="AC16Y1_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR5831286.1"
                     /translation="MFLGTHTPKLDEKGRLILPAKFRDPLTSGLVMTKGQERCVVIWP
                     SAQFDTYAESLRARSQNNEKVRAFTRVFFSSAFDDVADKQGRVTIPAPLREWAGLDRD
                     LVVVGADTRIEIWDAVAWSQDLAVQEPGFSSLDEVNL"
     gene            complement(2500..3417)
                     /locus_tag="AC16Y1_02085"
     CDS             complement(2500..3417)
                     /locus_tag="AC16Y1_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5831287.1"
                     /translation="MSFNFETSPKVNGKIALVTGGNSGLGYETVKALALKGAKVYLAS
                     RDITKGEKAKSEILTANPALDIEVLKLDLASIKSIENAVAEIKSKHNQLDFLINNAGI
                     MAMPYRETEDGFESQFGVNHLGHWVLTSSLMPLLLASEKSRVVTVSSTARHMAREITE
                     GDFNFKAKYSPWGSYGHSKLANYYFGIGLARILKRAKVSQASLIAHPGISNTNLQVQT
                     AAQGGAGAPGKYSETLAAKLGMSAWRGAQSQIRAALDPKATNGDFYGPRFAAFGDPVK
                     LTILKPGASKNIELLWRISREATGYAIDL"
     gene            complement(3470..4291)
                     /locus_tag="AC16Y1_02090"
     CDS             complement(3470..4291)
                     /locus_tag="AC16Y1_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 domain-containing
                     protein"
                     /protein_id="HIR5831288.1"
                     /translation="MRKLLNFLFGIFALFFVGFAAYSYISQSVIEEPLTIENVDGRPF
                     LTVTQPATDTWVGELDNSGYDISHPQCNSALPTGEVGFVIIGLNKGKPFSENPCFEKQ
                     WAWAKTYSAAAVYINTADPGDSDPAAYGQRIANDTIERLSKYGVGKQIPIWLDVETYN
                     TWTAPDRSVTVLSETMHILTQAGYHVGIYSVRSHWFEITLNAKIGVPTWVALGKFPDV
                     PSGVAAAKEACNQAGLAGSLPNIVQFVATVDGVTLDRNIMCTEPNGLVGIANQAQ"
     gene            complement(4338..4706)
                     /locus_tag="AC16Y1_02095"
     CDS             complement(4338..4706)
                     /locus_tag="AC16Y1_02095"
                     /inference="COORDINATES: protein motif:HMM:NF022684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HIR5831289.1"
                     /translation="MALSDHEQKLLEQMEKALANEDPRLVSTLRNPGIAVRTSKQVVL
                     AVIGVVAGILGLLAGVITKLPPLGIVGFVAIVAGLTALVSGIGSSALKNVGAKPTGQK
                     NKSSFMQGLEERWDRRNNEF"
     gene            complement(4813..6039)
                     /gene="dinB"
                     /locus_tag="AC16Y1_02100"
     CDS             complement(4813..6039)
                     /gene="dinB"
                     /locus_tag="AC16Y1_02100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIR5831290.1"
                     /translation="MTKEQLNRPNGPRGPLASDAGCNILHIDMDAFFASVEIRDNPKL
                     RDQPVIIGHGGGRGVVLSATYPARTLGVHSAMPMSRAMRIAPEAIVLEPSMHKYWEVS
                     EEVMQIFEDFTPLVQQLSVDEAFLDVSGAIKLLGSPTEIGQQIRKRVWEEQKITCSVG
                     IASTMFVAKLATNFAKPNGLHVVAKDQVIEFLHPLSVSALWGVGAKTAELLAKFGLYK
                     VSDVANLPIKTLKRIMGEVGGQHLHDLAWGRDPRVVTRDQEEQSIGNERTFDIDLQDP
                     DEITTHILALSNKVARRLREAGLTTKTISIKVKFHDFTSLTRSKTLSTPTDLSTEIYR
                     AARQLFERLKLERARVRLVGVRATGLSADAPIQMAFSDQATGWREAELALDKVAEKFG
                     ASAVKPARLIRTDADE"
     gene            complement(6085..7503)
                     /locus_tag="AC16Y1_02105"
     CDS             complement(6085..7503)
                     /locus_tag="AC16Y1_02105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5831291.1"
                     /translation="MPFSDEANPNVLPALADVVEESYWMDDDDWPDDKSRLIGENSAD
                     LLIIGGGFTGLWAAIDAKEHNPDLDVVLVEGKWIAYGASGRNGGFVAASLTHGFANGY
                     NRWPDELALLNKLGQDNLDAIEDFVKAEGIDCDFRRVGEIDVATEEYQLEDLQSFVEL
                     ANQYGEEYQWFDQATVRSKVNSPTYLGGLYNADGVALVDPARLAWGLRDSAIKRGVRI
                     FENTMITDLDERGNKVRAKADFGSITAPKVLLATNAFPPLLRRLKYLMVPVYDSVLVT
                     EPLSKAQREEIGWHEYQGISDSGNQFHYYRPTEDGSILWGGYDATYHWRSGFGADYER
                     TVDSWVKIGTHFFQTFPQLEGLKFTHAWSGAIDTCSRFSAFWGRTMGNKVAYVAGYTG
                     LGVGASRFGAQVAIDLLLDRDTDRRKLSMVNSLPIPFPPEPFRAAGINFTRWSLDKAD
                     RNQGKRNLWLRTLDRLGMGFDS"
     gene            complement(7528..8319)
                     /locus_tag="AC16Y1_02110"
     CDS             complement(7528..8319)
                     /locus_tag="AC16Y1_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5831292.1"
                     /translation="MMRWLGKNAVKIYAVIALIYLAIPVLYTFVFSFNDSGRMNLTWQ
                     GFTFAAWQNICGAPEVCSSVRNSIILSITSTILATILGTMIAFAIARFDFKGRKTTNL
                     LIFLPMATPEVVLGSSLLALYLNAGGGLLGFPGVLIAHVMFCISFVVVTVKARIASLD
                     PRLEQAAQDLYANERETFHKITLPLVLPGIAAAALLAFTLSFDDFIITNFNSGSNFST
                     FPKFVWLYNQRGIPPQANVISSLMFFLALTLVIVGQIRSSRKARA"
     gene            complement(8330..9244)
                     /locus_tag="AC16Y1_02115"
     CDS             complement(8330..9244)
                     /locus_tag="AC16Y1_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5831293.1"
                     /translation="MAFAHALADSSNKNSGTAAKAKRRRTPYILLLPGGLWLGIFFVI
                     PIFILFMTALQTPLPGGLPGQFVQTFTFSNFVEVLSRPQFLEALGRSFLYAAMATLLA
                     LAIGYPLAYAIAFKAGKYKNLMLIMVIAPFFVSFILRTIAWRQILGAEGPVLSLFRFL
                     GLASETSTIYPSAISVVAGLAYNFLPFMTLPIYASLDRMDVRLLEASNDLYANAFTTF
                     RKVTLPISLPGVVAGTLLTFIPAAGDYINSGLLGNQRTVMLGNVIDSRFLINVDYPTA
                     AVLSFTLMLSILILVAAYVRRAGTDELV"
     gene            complement(9247..>9612)
                     /locus_tag="AC16Y1_02120"
     CDS             complement(9247..>9612)
                     /locus_tag="AC16Y1_02120"
                     /inference="COORDINATES: protein motif:HMM:NF019998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HIR5831294.1"
                     /translation="YVLSRSRIAKGTDLTVGGKILVGIRPEKISVSTSDQLHGDFNNQ
                     TGVVRDASFFGVSTQYVIDTPSGEHWMAFEQNSGGSLIPAGTKVYATWKPEQAFGLPG
                     SEDVLAGDQIAAEFETAGN"
BASE COUNT         2695 a         2233 c         2093 g         2591 t
ORIGIN      
        1 ccatttacat cattaatgtt gccccgaatc gctggaagta aaactgtttg caagcggtta
       61 ctttggcttt tggcagctaa ctctggaccg cgaataattt gtaagttaac taactgtccg
      121 gcgtaaataa taaaaattcc agccagcact tgggtaaatc gaattactcg acgccgtgga
      181 cttgccaact gaaatactgc tggtggccta ggttcataaa aacgttgctg ttgaaatggt
      241 tgtgggacgg gtcgttgcac tcgctgcggt gcgggtcgtt gttgaggtcg taacggagtt
      301 tgcgaactca tggcaccacc ggagcaggtg ttggcggcac gaacggcact gcaggttctt
      361 tagctggaac aggctgtcca acaattactc gatctgaaag gcgtaagaac accggagttt
      421 cagcaggcac catgccgata gtgcgtgctg cagtaactaa attgtctgtt gattctgctg
      481 ctgcaacttc acttgctaaa cgttgttcgg tagccaacat ttgattcaac tcaatttgca
      541 gcgcaagttt ttcaaatgcc gcctgttgcg cttgagtgtt aattatcagc agaagaaagc
      601 ttccaaaaac taacatgcca aaaataattc cgacaaaagt atttcggtta gcccgacctt
      661 ttggaatacc aactggaatt gcgttagcaa ttggcgttac gatcgcgcgc gaattaaccg
      721 caactaaacg gctaactctt tttcgtcgcg tagggattgg gttaagtcgt ggtgcgcggg
      781 ctggcattgc actcatgcgg ccaacctttc aatcgcccga agtcgaactg aggcagcccg
      841 aggattttgg gcaatctcct cttcagtagc cagttctgca cttttagtaa gcagttgtag
      901 ttttggcttc tggccggccg gaataaacgg taagtccact ggaatttttg attgagttac
      961 ttcgcggaac gcgctcttta caattcggtc ttcgagtgat tgataggcaa gtacaactaa
     1021 ccggccacct atagccagtg cttcaattgc tgccggaatc gcgcgcgata aaaccgttaa
     1081 ttcatcattt acttcaatcc gtaaggcctg gaaagttcgc ttagctggat ttcctccagc
     1141 gcgacgtgtt gctgcaggaa tggcatcgcg gacaactgtg actagtcgat cacttgttgt
     1201 aaatagttga ttttcacgtt ctttaacaat tgcactagcg atgcgcgaag cgaaacgctc
     1261 ttcaccatat gtacttaaaa ttcttgccag ttcgccgtga ctataagtat tgactacatc
     1321 agctgcagtt tgaccctcgg ttggattcat ccgcatatca agtggagctg attgcgaata
     1381 tgaaaacccg cgttcggcac gatcaagctg catagaagat acgccaagat caaataggat
     1441 tccttgcaca gttggcatac caagttgggc taagacttct ggcagctcgt cgtagacagc
     1501 gtgaataaat tcgacgcgac cagcgaatgc ggctaagcga acctgggaat gtgtcagggc
     1561 atctttatcg cgatctaacc caatcacacg aagattcgga aaagtctcta atgctgattc
     1621 ggtatgccca ccgagtccga gagtggcatc aactagtact gccccatctt gctttaatgc
     1681 gggggctaaa agatctaaac agcgttgttt gaatactgga atgtgactat aagtagtcat
     1741 tagtcacact ccacaccaac aacctcaagt ccccagccgt ccatcagctt ggcaccgggg
     1801 aaggtgcgcc aagattttgt tacggctggg tacttgaggc gattggcaac atgattgttc
     1861 atagattgac ctcatcaagt gaggagaatc ctggttcttg aacagccaag tcctgactcc
     1921 aagcaactgc atcccagatt tcaattcgag tgtctgcgcc tacaaccact aagtcgcgat
     1981 ctaaacctgc ccattcacga agtggggctg gaatagttac tcggccttgt ttgtcggcaa
     2041 catcatcaaa tgcagatgaa aagaaaacgc gagtgaaggc gcgtaccttt tcattgtttt
     2101 gcgagcgggc tcttaatgat tcggcatagg tatcgaactg agcactcggc caaatcacga
     2161 cacagcgttc ttgacctttg gtcatgacta agccgctggt aagaggatca cggaatttag
     2221 ccggcagaat taagcggcct ttttcgtcca gcttcggagt gtgggtgccc aaaaacatgg
     2281 catacctccg aaaactgtgg caacccttcc ccgggccacc tcgttgaacc agaatactcc
     2341 actttgctcc actgtcaacc tatttgaata ccaattttca catttttcac actttttgca
     2401 ctttaaagcc ctatttaatg ggtggttgaa agtggaggtc ttatggcatg taaaaacccc
     2461 cgaaacaatt acttgaatcg ggggttagaa acgatgagtt taaagatcta ttgcgtaccc
     2521 agtagcttct ctagaaatac gccaaagtag ctcgatgttt ttgctggcac ctggcttcaa
     2581 aatagtcaat ttaaccggat cgccaaacgc ggcaaatcga ggaccataaa aatctccgtt
     2641 agtcgcttta gggtccaagg cagctctgat ttgtgactgg gctccccgcc aagctgacat
     2701 accaagtttt gcggccaggg tttcggagta tttacctggt gctccagccc caccttgcgc
     2761 agcagtttgt acttgaagat ttgtattcga aattcctgga tgggcaatta aactcgcctg
     2821 gctaactttt gcccgcttta aaatccgagc taaaccaata ccaaaataat aattagctag
     2881 ttttgaatgt ccgtatgacc cccaaggcga atattttgct ttgaaattga agtcaccttc
     2941 cgttatttct ctggccatat gtctggctgt acttgagacg gtaactactc tcgatttttc
     3001 actagccaac aacaaaggca ttagcgaact tgttaaaacc cagtgaccaa ggtggttcac
     3061 gccaaattgg gattcaaagc catcttcggt ttcgcggtac ggcatagcca taatgccggc
     3121 attattaatc aaaaaatcaa gttgattatg cttgctttta atttcagcta cggcattttc
     3181 aatactttta attgaagcca aatcaagttt aagtacttca atgtccaagg ctggattagc
     3241 tgtcaaaatt tcagatttgg ctttttcacc ttttgttata tctctactag ctaggtaaac
     3301 cttggcgcct ttgagagcta gtgccttaac agtttcatac ccaagaccgc tatttccacc
     3361 ggtgacaagt gcaatttttc catttacctt tggtgaagtt tcaaaattaa atgacattgg
     3421 cgctcctgtt aatagctgag tttaattatc tcaagttttt tagaggtact tattgggctt
     3481 ggttggcaat cccaactaaa ccattaggtt cagtgcacat tatgtttcgg tctaacgtca
     3541 caccatcaac agttgcaaca aactgcacaa tgtttggcaa gcttccagct aaaccagctt
     3601 gattacaggc ttcttttgcg gcggctactc cacttggcac atctggaaat tttcccaacg
     3661 ctacccaggt tggaacgcca atttttgcat tcaacgtaat ttcaaaccaa tgtgacctaa
     3721 ccgaataaat accaacgtga tagcccgcct gcgtaagtat gtgcatggtt tcgctcaata
     3781 ccgtcacaga tcgatcaggt gcagtccagg tgttgtaagt ttcgacatcc agccaaattg
     3841 ggatttgctt accaactcca tatttactta accgctcaat agtgtcattt gctattcgtt
     3901 gaccataagc tgctggatca ctatcaccag gatcggcagt atttatgtaa acggcagcag
     3961 ctgaataagt ttttgcccaa gcccattgtt tttcaaaaca tgggttctcg gaaaacggct
     4021 tacctttgtt caggccaatg attacaaaac caacttcacc ggtgggtagc gcggaattac
     4081 attgtggatg tgaaatgtca taaccactgt tatcaagttc accaacccaa gtatcagtgg
     4141 ctggctgagt aacagttagg aatggtcggc cgtctacgtt ttcaatggtt agtggctctt
     4201 caataaccga ttgagagata tacgaataag ctgcaaaacc aacaaagaac aatgcaaaga
     4261 ttccgaataa gaaattgagt aatttacgca tcagttaagt tgggtactcg aaaaaatttt
     4321 taaataggct taaaaattta gaattcgtta ttgcggcgat cccagcgctc ttcaagcccc
     4381 tgcatgaacg aagatttatt tttctgtcca gttggcttag ccccgacatt tttcaaagct
     4441 gaactgccga ttccacttac tagcgccgtt aggccagcaa caattgccac gaagccaacg
     4501 atgccaagtg gtggcaattt ggtgataact ccagcaagta gtccaagaat tccagcgact
     4561 acgccaatta cagccaacac cacttgcttc gaggtgcgta cagcaatccc tggatttcgc
     4621 agcgttgaaa ctagtcttgg atcttcattg gcaagtgctt tttccatttg ttctaagagt
     4681 ttttgctcat gatctgaaag tgccatgtgg gttaactcct tgtctctgaa ctggaaattg
     4741 gccgttactt aaaggatacg tccgaactac caatcctgca gaattgtgcc agaaaatgga
     4801 catatttgtg gattattcat ctgcatcggt tctaatcaat ctggccggtt ttacagccga
     4861 ggcgccaaat ttttcggcca ctttatctag agctagttca gcttcgcgcc agccagttgc
     4921 ttgatcacta aacgccatct gaattggcgc atcagcagaa agtccggtgg cccgcacgcc
     4981 aactaaccgg accctggctc tttctaattt aagtcgttca aataactgcc tagctgctcg
     5041 atagatttcc gtacttaaat ctgttggagt acttagtgtc ttacttctag tcagcgaagt
     5101 aaaatcatga aatttaactt tgattgaaat tgttttagta gttaagccag cctcgcgaag
     5161 tcgacgtgca actttgttac ttagcgcaag aatgtgcgtt gtaatttcat caggatcttg
     5221 tagatctata tcaaaagttc tttcgtttcc aatactttgt tcttcttgat ctcgcgtgac
     5281 gactcgagga tcgcgacccc aagctaaatc atgtagatgt tgtccaccta cctcgcccat
     5341 gattcgtttt aaagttttaa ttggtaaatt tgcaacatcc gaaactttat aaagaccaaa
     5401 tttggcaagt agttcagcag tttttgcgcc tactccccac aatgcactaa ccgataacgg
     5461 gtgcaaaaac tcaataactt gatcctttgc aactacatga agtccgttag gttttgcaaa
     5521 attagttgca agttttgcga caaacattgt cgaggcaatt cctaccgagc aagttatttt
     5581 ttgctcttcc cagacacgct tgcgaatttg ttggccaatt tcagttggac tgccgagtaa
     5641 tttaattgct cctgacacat ccaaaaatgc ttcatcgacg gatagttgct gaactagtgg
     5701 cgtgaagtct tcgaaaatct gcataacttc ttcggagact tcccagtatt tatgcatact
     5761 tggttccaac acaattgcct cgggggcgat tcgcatagcc cgggacattg gcatcgcaga
     5821 atgcacacct aaggtgcggg ctggatacgt tgccgataac accacccctc gaccgccgcc
     5881 atgtccaata attactggct ggtcgcgcag ttttgggtta tctcgaattt ctactgaagc
     5941 aaaaaacgca tccatgtcga tgtgcaagat attgcaacca gcatcactag ctaacgggcc
     6001 gcgcggtccg ttagggcgat ttagttgctc tttagtcatg ggttaattgt tacacctagc
     6061 tcagacgtaa cgaattttaa ataactaaga gtcaaagccc atgccaagcc gatcaagagt
     6121 ccgtaaccac aaattacgtt taccttgatt acgatcagct ttgtctagtg accagcgagt
     6181 gaaattgata cctgcagccc gaaaaggctc tggtgggaaa ggaattggca aactattcac
     6241 cattgaaagt ttgcgtcgat cagtatcccg atctagtaaa agatcgatcg caacttgcgc
     6301 gccaaagcgc gaagcaccaa ctccaagtcc ggtataaccg gcaacataag caactttatt
     6361 gcccatagtc ctaccccaga acgcgctaaa tcgtgagcaa gtatcaattg cgccgctcca
     6421 ggcatgcgtg aatttcaatc cctcaagttg tgggaaagtt tggaaaaagt gtgtcccgat
     6481 ttttacccaa gaatcaacag tgcgttcgta gtccgcgcca aatccacttc gccaatgata
     6541 ggtagcgtcg tagccacccc aaagtattga accatcttct gttggtcggt agtaatggaa
     6601 ctgattgcct gagtcactta tcccttggta ttcatgccaa ccaatttctt cacgctgagc
     6661 tttagaaagg ggctcggtca ccaataccga gtcataaacc ggcaccatta aatatttaag
     6721 tcggcgcagc agcggtggga atgcattcgt ggccaacaag actttaggag cagtgattga
     6781 gccaaaatct gctttggctc gaactttatt tccacgttca tcaagatcag taatcatcgt
     6841 gttttcaaat atccgcacac cacgcttgat cgcactatct ctaagacccc aagccagcct
     6901 ggctggatct acgagcgcaa caccgtctgc gttatacagt ccacctagat aagttggtga
     6961 attaactttg cttcgcacag tggcctgatc aaaccattga tactcttcac catattggtt
     7021 ggctaactca acaaaacttt gtaaatcttc aagttggtac tcttcggtcg caacatcgat
     7081 ttcgcccact cggcgaaagt cgcaatctat cccctcggct ttaacaaaat cttcaattgc
     7141 atctaaatta tcttgaccaa gtttgtttaa tagggctaac tcatctggcc aacggttata
     7201 gccatttgca aatccgtggg ttaatgacgc agcaacgaaa ccaccatttc gtcccgatgc
     7261 cccataggca atccatttac cctcgactaa aaccacatcg aggtctgggt tatgttcttt
     7321 ggcgtcgatt gctgcccata atccggtaaa accgccacca ataattaata agtcagctga
     7381 gttttcgcca attaaccgag atttatcatc gggccaatca tcgtcatcca tccagtagga
     7441 ctcttcgaca acatcagcta gagcaggtag aacatttgga tttgcttcgt ccgagaacgg
     7501 cataaatcac cagcaggtaa ggattactta cgcgcgcgcc tttcgtgaag atcgtatttg
     7561 gccaactatt accaaggtta atgccaaaaa gaacattaga gaggatatga cgttcgcctg
     7621 gggtggaata ccgcgctggt tataaagcca aacaaacttc gggaaggttg aaaaattaga
     7681 gcctgaattg aaatttgtaa tgatgaaatc atcaaaagac aaagtaaaag ctagtaatgc
     7741 agccgctgcg ataccaggca aaactaacgg caaggtaatt ttgtggaaag tttcgcgctc
     7801 gtttgcataa aggtcttgcg cagcttgttc taatcgtggg tcaagtgagg caatacgggc
     7861 tttcacggta accaccacaa atgaaataca aaacatcaca tgtgcgataa gaacacccgg
     7921 gaagccaagt aagcctccgc ctgcatttag ataaagcgcg agtaatgacg agccaaggac
     7981 tacttctggt gtagccattg gtaaaaagat caatagattt gtggttttgc gacctttgaa
     8041 gtcaaagcga gcaatcgcaa aggcaatcat ggtgccaagg atggtggcca aaatagttga
     8101 ggtaatactc aaaatgatcg agttacgcac cgaagaacag acttcggggg caccacaaat
     8161 attttgccaa gccgcaaaag taaagccttg ccaggttaag ttcatccgac ctgaatcgtt
     8221 aaaggaaaaa acaaaagtat aaagaactgg aatggctaaa taaataagcg caattacggc
     8281 gtagatcttc accgcatttt tgcctaacca acgcatcatc tacacatcct taaaccaact
     8341 catccgtacc agcacgacga acataggcag ctaccaaaat caatatcgac aacatcagcg
     8401 tgaaggaaag aactgcagca gttggataat caacatttat taagaatcga gaatcaatta
     8461 cgttacccaa cattacggtg cgctggttac ctaataagcc agagttaatg taatcgccag
     8521 ctgcgggaat aaaagtaagt aaggttccag cgactacacc cggtaatgaa attggcagcg
     8581 tgactttgcg gaaagtagtg aaagcatttg cgtacaaatc gttcgaagct tccagtaggc
     8641 gtacatccat gcgatcaagc gaagcataaa ttggcaaagt cataaatggt aagaagttat
     8701 aagcaagtcc ggctactaca gaaattgcag atggatatat tgtgctcgtt tcactggcta
     8761 agccaaggaa tctaaagaga gaaagaaccg gtccctcggc acctagtatt tgacgccaag
     8821 cgattgtgcg caaaataaaa gaaacgaaaa atggtgcaat caccataatt aacatcaagt
     8881 ttttatactt accggcttta aaagcaatgg cgtacgcgag tggataccca attgccaaag
     8941 cgagcaatgt tgccattgca gcatataaaa atgatcggcc aagggcttct aaaaactgag
     9001 gtcgactaag aacttcgacg aagtttgaaa atgtaaacgt ctgaacaaac tgtccgggca
     9061 atcccccagg aagcggagtt tgtagcgccg tcatgaacaa aataaatatt ggaattacga
     9121 aaaagattcc tagccagagt ccgccaggaa gtaaaagtat gtacggggtg cgtctgcgct
     9181 ttgcttttgc ggcagttccg gaatttttat tggaactatc cgctagcgca tgagcaaatg
     9241 ccatatttaa ttgcctgctg tttcaaattc tgcagcgatt tgatcgccag caagtacgtc
     9301 ttcacttcca ggcaaaccaa atgcttgttc tggtttccaa gttgcataga cttttgtgcc
     9361 agccggaatt aacgagccac ctgaattttg ctcgaatgcc atccaatgtt cgcctgatgg
     9421 cgtatcaatt acatactgcg ttgaaacgcc aaagaacgat gcatcgcgaa ctacgccagt
     9481 ttgattatta aagtcaccgt gtaattggtc gctggttgag acgctaattt tttctgggcg
     9541 aattccgact aaaattttgc cgccgacagt tagatcggtt cccttagcaa ttcggcttcg
     9601 gctcaatacg ta
//
LOCUS       DBNHVN010000114         3919 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_2026167, whole genome shotgun sequence.
ACCESSION   DBNHVN010000114 DBNHVN010000000
VERSION     DBNHVN010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_2026167"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="AC16Y1_02125"
     CDS             <1..463
                     /locus_tag="AC16Y1_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017585712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831295.1"
                     /translation="ARRWLVAIAVGVATYFLLGLPTVVVENNLFGREIGLTSWSIPVL
                     VATSVLSGLLFATYVRVGEVPTTEREARLGSLGGFFSFLAIGCPVCNKLALIALGYSG
                     AIKYFAPIQPYLAAIGIGLLTYALRQRLTNEASCRLPVKVTKQEMKINEHN"
     gene            501..794
                     /locus_tag="AC16Y1_02130"
     CDS             501..794
                     /locus_tag="AC16Y1_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020490561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HIR5831296.1"
                     /translation="MPAKTKKISHVVQDSDALDEFTKRLRRAEGQIGGVIRMVEDGRP
                     CEDIVNQLAAVSKAVNKAAFGLITAGLSECILEEGKDAAEVQSQLQKLFLTMV"
     gene            812..2440
                     /locus_tag="AC16Y1_02135"
     CDS             812..2440
                     /locus_tag="AC16Y1_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5831297.1"
                     /translation="MKKIVIVGGVAGGMSAATRLRRLDPDASITVIERSGFVSYANCG
                     LPYYVGGVIADQASLLLQTPQSLDARFELDVRIESEVTSIDRTNKKLSVQTKSGESYE
                     LEYDTLVLSPGAQPIIPPIEGIERAHTLRTVEDVTRIAAAVASNPKSAVVIGGGFIGV
                     ESAENLVHKKIQTTIVEAAPQLLGPIDPELAQLVHTEVLKHGVHLRLGAGVTKITDNS
                     VQLADGSEIDAQLIICAIGVKPDTTLAKSCDLEIGERGGITVNEFGQTSDPNIYAIGD
                     VAEKIDAFDGEATLVPLANIANRQGRVAADHIMGRTVRPVKTIGTAIVKVFDLTVAST
                     GWNEKRLKARGINYQAIHTHPGSHAGYYPGSEQMALKLLVEKPNGKILGAQAVGGEGV
                     DKRIDVIATAIRCELPAPELADLELAYAPPFGSAKDPVNMLGYIAENLLTGFTENVQW
                     YDLRPMQSQGAIVLDVRTEAEFARGAISGSINIPLDDLRNRFNEIPDGKILVYCQVGQ
                     RGHTATVLLRERGVNAVNLDGGYKTWVAAEPLTV"
     gene            complement(2437..3441)
                     /locus_tag="AC16Y1_02140"
     CDS             complement(2437..3441)
                     /locus_tag="AC16Y1_02140"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5831298.1"
                     /translation="MIKRKYFEYLTKLIPISFIAWLIYSAVRYPNEFATSVVLGIAKG
                     SIYALIAVGFGLIYNTTKILNLAHGEMFMLGAVGSTYFLIDQFGANTATVTNFLLLFI
                     AVVLAMILGASVSWLTNAAVFSHLNTKQKMAPVVASLGIALIIQNLAIKWNGSGVKKF
                     ASIIPPELPYRAFWPSVERTLVVLVITLPIIFVAMWVITKSRNGLAIRAISDDVATSQ
                     LMGINVSRTISWTYLIGGACAGAAGVIYAQQYQISNYSLGMEVGLIAYAAAIIGGVGQ
                     IKGTVLGGLLIGVVEALSDGVTSGLGYRWSQTAIYAVFILMLVYKPQGLWGTKESESV
                     "
     gene            complement(3464..>3919)
                     /locus_tag="AC16Y1_02145"
     CDS             complement(3464..>3919)
                     /locus_tag="AC16Y1_02145"
                     /inference="COORDINATES: protein motif:HMM:NF020296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIR5831299.1"
                     /translation="EFQYIACDLLIKNSNRLSQDFVIKQAKWFITNKSWWDTVDAIRT
                     AIEPVIDRNPQLRRLMDQWIKYDNAWLVRVAIIHQLKLGKRTDVARLEKYCARRATDT
                     EFFIAKAIGWALRAYSYTDPRWVKRFVKAHPELQPLSKREALKAIQRTK"
BASE COUNT         1145 a          834 c          832 g         1108 t
ORIGIN      
        1 agcacgtcgt tggcttgttg caatagcagt cggagttgca acttatttcc tacttgggct
       61 cccgaccgta gtcgtcgaga ataacttatt cgggcgtgaa attggtttga ctagttggtc
      121 cattccagtt ctggttgcaa cttctgtttt atctggactc ttgttcgcaa cctacgtaag
      181 agtcggtgaa gtcccaacta ccgagcgaga agcaagactt ggcagtctcg gtggattctt
      241 ttcatttctt gcaattggtt gcccagtatg taacaaacta gctttgattg ccctgggtta
      301 ctccggtgct attaaatact tcgcaccaat tcaaccgtac ttagctgcaa ttggtattgg
      361 gctacttacc tacgcgctcc ggcaaagatt aacgaatgaa gcgtcttgca gacttccagt
      421 aaaagtaact aaacaagaaa tgaaaataaa tgagcacaat taaattgatt aaaaaatttt
      481 cgatcggtta ggaaactaaa atgcctgcaa aaactaaaaa gatttctcat gttgtgcaag
      541 actctgatgc tttggatgaa tttactaaac gccttcgacg tgcagaaggc caaattggtg
      601 gcgtaattcg aatggtagaa gatggccgac cttgtgaaga catcgtcaac caattagctg
      661 ctgtttcaaa agcagttaat aaagctgctt ttggtttaat tactgcgggg ctttcagaat
      721 gcattcttga agaaggtaag gatgcagcag aagttcaaag tcaacttcaa aaacttttct
      781 taaccatggt ttaactgtta ggacccaaac gttgaaaaaa attgtcatag ttggtggcgt
      841 tgccggtggg atgtctgccg caactcgatt gcgcagactt gatccagatg cgtcaattac
      901 cgttattgag cgaagtggct ttgtctctta cgcaaactgt gggcttcctt attatgtggg
      961 cggtgtcatt gctgatcaag catcgctgct attgcaaact ccacaatcac ttgatgctcg
     1021 attcgaatta gatgtccgaa ttgaatcaga agtaacctcg atagatcgaa ctaataaaaa
     1081 actttctgtt caaactaaat cgggtgaatc gtacgagctt gaatatgaca ctttagtttt
     1141 aagcccaggt gctcaaccaa taattccacc tatcgaaggg attgagcgag cacatactct
     1201 tcgaactgtg gaagatgtaa ccagaattgc ggctgcagtt gctagtaacc caaaatctgc
     1261 agttgttatc ggtggcggat tcatcggtgt tgaatcagca gaaaatttag tacataaaaa
     1321 aatccaaacc acaattgttg aggctgcccc gcaactgctc ggacctatag atcctgaact
     1381 agcccaactt gtgcatactg aagttttgaa acatggtgtg caccttcggc ttggcgcagg
     1441 cgtaacaaaa attactgata actccgtaca acttgctgac ggaagtgaaa tcgatgcgca
     1501 actaattatt tgcgcaattg gagtaaagcc agatacgact ctggctaaat cgtgtgatct
     1561 agagattggc gaacgaggcg gcattacggt taacgaattt ggtcagactt ctgatccaaa
     1621 tatttatgca ataggtgacg tcgctgaaaa aatcgatgca ttcgatggtg aagcaactct
     1681 tgtgccactt gcaaacattg caaatcgtca aggtcgcgtt gcagcagatc acatcatggg
     1741 tcgcactgtt cggccagtga aaacaatcgg taccgcaata gttaaagttt ttgacttaac
     1801 tgttgcctcc acaggttgga atgaaaagcg acttaaagca cgcggaatta actaccaagc
     1861 aattcacacg cacccgggat ctcatgctgg ttactaccca ggtagtgaac aaatggcatt
     1921 gaaacttttg gttgaaaagc caaacggaaa aatacttggc gctcaagcag ttggtggtga
     1981 aggtgtagat aaacgtattg acgttattgc tactgcaata cgttgtgaat tgcctgcgcc
     2041 agagcttgct gacttggaat tggcctatgc cccacctttt ggttcagcaa aggatccggt
     2101 aaacatgctt ggttacattg ccgagaattt actcaccggt tttaccgaaa atgttcagtg
     2161 gtatgacttg cgaccaatgc aatcccaagg cgcaattgtc ttagatgtgc ggacagaagc
     2221 agagtttgct cgaggtgcaa tttctgggtc gataaacatt ccgctcgatg atttgcgtaa
     2281 tcgatttaac gaaataccag atggaaaaat tttggtttac tgtcaggttg gtcaacgtgg
     2341 tcatactgca actgtcttac ttcgtgaacg tggcgttaat gccgttaacc ttgatggtgg
     2401 gtataaaact tgggttgctg ccgaaccact aactgtttaa acgctttcac tttctttagt
     2461 gccccataac ccttgcggtt tatagaccag catcaagata aacacagcat aaattgcagt
     2521 ttggctccag cgataaccca aaccggaagt tacgccatcg ctaagtgctt cgactacccc
     2581 aatgagtaag ccaccaagta ctgttccctt tatttgcccg actccaccga ttattgctgc
     2641 agcataagca attaaaccga cttccattcc gagtgagtag ttcgaaattt gatactgctg
     2701 cgcataaatt accccagccg cgcctgcgca tgcaccgccg attagatacg tccaacttat
     2761 ggtgcgagaa acatttatcc ccattaattg agaagttgcg acatcgtcgg aaatcgctcg
     2821 aattgccaat ccatttcttg atttagtaat aacccacatc gctacaaaaa ttatcggcaa
     2881 ggtaatcaca agaactacca aagttctttc cactgatggc caaaatgctc ggtacggtaa
     2941 ttcaggcgga attatcgaag cgaatttttt aacacctgac ccattccatt tgattgcgag
     3001 gttttgaatt attagcgcaa tacccaaaga tgctacgacg ggtgccattt tttgttttgt
     3061 attcaagtgt gaaaagacag ctgcgttagt taaccaactc actgatgcac ctaagatcat
     3121 ggcaagtaca accgctataa ataacaacaa gaaattggtt acggtggctg tattcgcacc
     3181 gaattgatca attaagaaat aagtcgatcc aacggcaccc agcataaaca tttcgccatg
     3241 tgccaagttc aaaatttttg tagtgttata aattaagcca aaaccaactg caattaatgc
     3301 ataaattgaa ccttttgcaa ttccaagtac aaccgatgtg gcaaattcat ttgggtaacg
     3361 aaccgcactg taaattaacc aggcaataaa actaattgga ataagtttcg ttaagtattc
     3421 aaaatactta cgcttgatca tttaccaagt ctaatttcga ttatcatttg gtgcgctgaa
     3481 ttgcttttaa agcttcgcgc tttgaaagtg gttgtaattc gggatgagcc tttacaaaac
     3541 gcttaaccca tctcggatcg gtatacgagt aagcacgtag cgcccaacca attgccttag
     3601 cgataaagaa ttcagtatct gtagcacgcc gggcacaata tttttctaat cgagcaacat
     3661 cagttcgttt accaagtttc aattggtgaa ttattgctac gcgcactagc caagcattgt
     3721 catacttaat ccattgatcc atcaatcttc gaagttgagg gttgcgatca attactggtt
     3781 caattgctgt tcgaatcgca tcaacggtat cccaccatga tttattggtg ataaaccact
     3841 tggcttgttt aattacaaaa tcttgcgata accgatttga attttttata agcaaatcgc
     3901 acgcaatata ttggaattc
//
LOCUS       DBNHVN010000115         2677 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_612475, whole genome shotgun sequence.
ACCESSION   DBNHVN010000115 DBNHVN010000000
VERSION     DBNHVN010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_612475"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1666
                     /locus_tag="AC16Y1_02150"
     CDS             <1..1666
                     /locus_tag="AC16Y1_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007449499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HIR5831300.1"
                     /translation="AFRIATQLVDEHLITMDEALTRVNGSQLVQLMFPQFDSANQTKP
                     VATGMAASPGAAVGKAVFDSATAVRWTERGESVVLVRRETNPDDLEGMIVANGILTSR
                     GGKTSHAAVVARGMGKTCVCGADAIEVNTAEKCARIGDLQINEGDMISIDGSSGQVFI
                     GELSVVPSPVSIYLENGLDYAISNSDEETADLIKAVDRILNHADSVRRLRVRTNADTA
                     EDSSWARKLGAEGIGLCRTEHMFLGDRRVLIERVILADNETDRNTALDALLPMQQHDF
                     EQILEAMNDLPVTIRLLDPPLHEFLPDRAQLMVDVALGKERGQDVSHQELLLTAVERL
                     HESNPMLGLRGVRLGIVIPGLFVLQVRAIAYAAINRMRQGGNPKVEIMVPLVGSVMEL
                     HLIRDEIDKVIRDISQAENIDLPFAIGTMIELPRAALTAQRIAEAAEFFSFGTNDLTQ
                     TTWGFSRDDVEAAFFANYLDKGIFTVSPFESLDVDGVGALIATAVEKGRATRPDIKLG
                     VCGEHGGDPESIKFFHAVGLDYVSCSPFRVPVARLEAGRVSVLSRI"
     gene            complement(1663..>2677)
                     /locus_tag="AC16Y1_02155"
     CDS             complement(1663..>2677)
                     /locus_tag="AC16Y1_02155"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5831301.1"
                     /translation="LGIFFAMKPAGHWAAKYGSSRVLVHATWAYGLSTILLGLLLSVP
                     WLIGTLFLTGFLVAVHDIAMNSHATAIERISNKSLMNSFHARFSVGGLVGAAIGGTFS
                     QLEISYLTQAIMLAVISVAAVPILQKNLLPPQVDIHEEELAPKADRERPHVFYWLGLL
                     GFAASVCEGAAADWGAILLRDTWQASPFISTIPFIAFSAAMVIGRLSGDRITDRFSRE
                     TVVKFGGYIAGIGLASGLFIGETFGITLGWVLLGIGVSVIIPSLFSAASEIARTRFVG
                     HIAPSQAVAIVGGVSYAGFLVGAPLLGFLSEYLTLRWSLLIPAVAAILMGTFAKLVRS
                     NPS"
BASE COUNT          772 a          600 c          616 g          689 t
ORIGIN      
        1 tgcattcagg atcgccaccc aattagttga tgaacacttg atcacaatgg atgaagcgct
       61 tactcgagtt aatggtagtc aactagttca acttatgttt ccgcagtttg attcagcaaa
      121 tcaaactaaa ccagttgcaa caggaatggc tgcgtcgcca ggcgctgctg ttggcaaagc
      181 agtttttgat tcggcaaccg cagtccgttg gacagaacgt ggtgagagtg ttgttttagt
      241 tagacgtgaa accaacccag atgatctcga gggcatgatt gtggccaacg gtattttgac
      301 ttctcgaggc ggtaaaactt cacacgctgc agtagtcgct cgtggtatgg gtaaaacttg
      361 cgtctgtggt gcggatgcaa ttgaggtaaa tactgcagaa aaatgtgcgc gcatcggtga
      421 tttgcaaatc aatgaaggcg acatgatttc aattgatggt tcatcgggcc aagtttttat
      481 tggcgaactt tcagtggtgc catctcctgt ttctatttat ctagaaaatg gtttggacta
      541 tgcaatctcc aattctgatg aagaaaccgc tgacttaatc aaggccgttg atcggatttt
      601 gaaccatgca gactcagttc gccgcctaag agtgcgaacg aatgctgaca ctgctgaaga
      661 ttcaagttgg gctcgaaaat taggcgccga aggaatcggc ctatgccgta ctgaacatat
      721 gttcttgggt gatcgacgtg tactaatcga gcgcgttatt ttggctgata acgaaaccga
      781 tcgaaatact gcgctagatg cgttgctgcc aatgcaacag catgactttg agcaaatcct
      841 cgaagctatg aacgatctgc cagtcactat ccgtttgcta gatccaccgc tgcatgaatt
      901 tctgcctgac cgtgctcagc taatggtaga tgtggcactt ggtaaagagc gcggccaaga
      961 cgtatctcat caagagctac ttttgacggc tgttgaacga ttacatgaat cgaacccaat
     1021 gcttggcttg cgcggtgttc gcctaggaat tgtgattcca ggattatttg tcctacaagt
     1081 aagggctatt gcttacgccg caattaatcg catgcgccaa ggtggaaatc caaaggtaga
     1141 aataatggtc ccgctagttg ggtcagtaat ggaattacac ctaatccgag atgaaataga
     1201 caaagttatt cgagatatct ctcaagcaga aaatatcgac ctaccgtttg caattggcac
     1261 aatgattgaa ctgccgcgag ctgcacttac cgcgcagcga attgccgaag ctgctgaatt
     1321 cttttcgttt ggcacaaacg atttaacgca aacaacttgg ggtttcagtc gtgatgatgt
     1381 tgaggccgca ttctttgcaa attatttaga caaaggtata ttcacagttt caccttttga
     1441 atcactcgat gttgacggtg ttggtgcttt aattgccact gccgtagaaa aaggtcgagc
     1501 aactagacct gacattaagt taggggtctg tggcgaacat ggcggtgatc ctgaatccat
     1561 taagtttttc catgctgttg gacttgatta cgtttcttgt tcaccgtttc gagtaccagt
     1621 agccagactg gaggctggtc gagttagcgt actttcaaga atttaacttg gattagatct
     1681 aactagtttt gcaaaggttc ccatcaagat tgctgcaaca gctggaatca ataatgacca
     1741 gcgcaacgtt agatattcag ataaaaatcc aagcaaaggt gcacccacca agaaaccagc
     1801 gtaagaaacc ccaccaacta ttgccactgc ttgtgacggg gcaatgtggc cgacaaaacg
     1861 agtgcgggca atttcactag cggcgctaaa cagcgaagga ataattaccg acacacctat
     1921 tccgagcaaa acccaaccca gagttatgcc aaaagtttca ccaataaata aaccgctggc
     1981 taacccaata cctgcaatgt agccaccaaa tttaactact gtttcgcgtg aaaatcgatc
     2041 agtaattcga tccccactta atcgaccaat aaccatggcc gctgaaaacg cgatgaacgg
     2101 aattgtagag atgaatggtg aagcctgcca agtgtcccga agcaaaattg caccccagtc
     2161 agcagctgca ccttcacaaa ctgaagcagc aaaaccaaga agtcccagcc aataaaaaac
     2221 gtgtgggcgt tcacgatccg cctttggtgc taattcttct tcgtgaatgt ctacctgggg
     2281 tggcagtagg ttcttttgca aaatgggcac agcagcgact gaaatcaccg ccaacataat
     2341 cgcttgagtt aagtaactta tttctaattg agaaaaggtt cctccgatag ccgcgccaac
     2401 taatccgccg acactaaatc tggcatggaa actattcatc agtgatttat ttgaaatacg
     2461 ttcaattgct gttgcgtgtg aattcattgc aatgtcatgt accgcgacta aaaatccagt
     2521 aagaaaaaga gtgccaatta accagggcac actaagcaat aagccaagca aaattgtaga
     2581 taggccatac gcccaagtgg catgcaccaa aactcgagaa cttccatatt ttgctgccca
     2641 atgcccagct ggtttcatgg cgaaaaatat gcctaag
//
LOCUS       DBNHVN010000116         9457 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1878169, whole genome shotgun sequence.
ACCESSION   DBNHVN010000116 DBNHVN010000000
VERSION     DBNHVN010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 9457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9457
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1878169"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /gene="mshD"
                     /locus_tag="AC16Y1_02160"
     CDS             <1..905
                     /gene="mshD"
                     /locus_tag="AC16Y1_02160"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: protein motif:HMM:TIGR03448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HIR5831302.1"
                     /translation="SDEVVSEIKKVINAATVFDEMAPLSEHVLIHLNQGGDEFDEHFL
                     AKDFTGKIIGYLHLDRTDEVAGPIIEVVVHPDSRNNGIGAQLVNYATQTIKDSRMRLW
                     AHGELGGAYALAKKLGFAKSRELWQMRRSLYAELPKLELPSSIQIKNFLPEENMKEFL
                     ILNSIVFEKHPEQGRLDEKDFKIRMSETWFRSEGFFLAQDRDKKLIGFNWTKIHGQDS
                     GPTNNYGHQKIGEIYVLGVKPGNRQSGLGKALAIRGLEYLREQGLGAAMLYVDRTNVP
                     AIKLYESIGFAHWDTDVMFQKQTS"
     gene            complement(898..1587)
                     /locus_tag="AC16Y1_02165"
     CDS             complement(898..1587)
                     /locus_tag="AC16Y1_02165"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HIR5831303.1"
                     /translation="MKPGPIALVGSGEYLPVMAQIEGNLISGRGNKYVQIPTAAAQEG
                     ERSLNYWRDLGIAQANRLGVQPVPLMITDRTKADDDEFVKQIEGAGLIYFSGGNPHYL
                     ADTLRDTKLWTAIYEAWLAGTALAGCSAGAMAIANHIPELRSFMRTTTPGFGLLPNVR
                     VLPHFDRMFMNFVSRIKHDKELTVVGIDENTALVGGLDEWEVQGVGSVWVFFAGNKTQ
                     YKSGEKVVLPS"
     gene            complement(1584..2747)
                     /locus_tag="AC16Y1_02170"
     CDS             complement(1584..2747)
                     /locus_tag="AC16Y1_02170"
                     /inference="COORDINATES: protein motif:HMM:NF015534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysS/YqeB C-terminal domain-containing protein"
                     /protein_id="HIR5831304.1"
                     /translation="MSRQIVIMGSGETSPTMVTPHQQIWSATDTTEEYAFFLDTPFGF
                     QENADELTSRISDYFAKSVGLNPKPVQLRQANDSPAAIANAVNQLRNAKWIFAGPGSP
                     SYALKVWRQTGLQNQLEKVLEQGTLVFASAAALTIGTKTIPVYEIYKAGEDPYLLDGL
                     NILEKFTGLDAVVVPHFDNAEGGTHDTRFCYMGERRIRLLEAQLPETTFILGVDEHTG
                     IKFDLDAQNVTVFGRGNLTVRKGEHTWTLPSGQSAAISELAEQGGVKRTVSSTPIQLP
                     TQPDAVERLLESGQVLAAVDALLEIDDLDRDLETRAIVHGLITRLGQMAANPRVDINS
                     VVGPYIEMLLHARQAARQSGRWDEADEIRDQLTSLKVTIKDSAEGSTWDIDAS"
     gene            2878..5022
                     /locus_tag="AC16Y1_02175"
     CDS             2878..5022
                     /locus_tag="AC16Y1_02175"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003953549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HIR5831305.1"
                     /translation="MASQPPQVNLPGDRYLNREMSWLAFNRRVLELAQDENMELLERV
                     KYISIFASNLDEFFMVRVAGLKRRIETGLARKDSSGMQPRELLDEILAESYALMQEHA
                     ATFNNSIMPSLVANGIEILRWEELTTEERERMSNLFAEQIFPVLTPLAVDPSHPFPYI
                     SGLSLNLAISVKNPETEHELFARVKVPASLPRFFEVSRERFVPLEDVIAGHLDMLFPG
                     MEILQHHTFRVTRNEELEVEEDDAENLLQALEKELSRRRFGAPVRLEVENTIDPHILE
                     LIVSEVGVEQNEVFRITGPRELESLVELAKLNREDLKAPVFVSKTSTELAEVESATAP
                     NVMEVMRNKDVLLHHPYDSFSTSVQLFVQQAAEDPTVLAIKQTLYRTSGDSPLVNALI
                     TAAQNGKQVLAVVEIKARFDEQNNIEWARKLEEAGVHVVYGIVGLKTHAKLCLVVRND
                     AGKLNRYVHIGTGNYNPKTARHYEDYGLLTTDPKIAEDVASLFNLLSGYGIQGKYKRL
                     IVAPKELRNTLVEKIDREAANAWIGLTSGIRIKCNAIVDETIMDALYRASAAGVKIEI
                     LVRGMCALKPGVPGLSENISVRSILGRFLEHSRVYEFMNAGDREVWIGSADLMHRNLD
                     RRVEALVQIKQPNASAIIQVLDLAFAKTTSAWELMPGGNWNRQNLDEDGELLTDYQEF
                     LINNHPVPRSGIDHPTPRVFNALLNKVGLGER"
     gene            5012..5929
                     /locus_tag="AC16Y1_02180"
     CDS             5012..5929
                     /locus_tag="AC16Y1_02180"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5831306.1"
                     /translation="MSDKYIIAAGAVVTRQNAGQTEYLVIHRGYRDDWSFPKGKVDPG
                     ENILGTAIREVREETGYAIKLGIPLPTQKYKVANGNKESHYWHATVTGGNFVANDEVD
                     HLEWLSLEQAQKRLSYEHDHEVLLAAAHAKPTTPFIVLRHTQAIKRAEWVVSKEGLAE
                     VDASRPLTAVGRMQAAGLVPALAAFGINELHSSDSRRCRDTVGPYASARSISIELEPT
                     LSEERHKEHPDKALDRVAQLAQTTEALALCTHRPVMPTVMESLGGIFQIENPGKKTFD
                     PALTPGSMVIFHRESDNLMNVLSVERHIH"
     gene            complement(5933..6751)
                     /gene="pstB"
                     /locus_tag="AC16Y1_02185"
     CDS             complement(5933..6751)
                     /gene="pstB"
                     /locus_tag="AC16Y1_02185"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018800075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR5831307.1"
                     /translation="MPVKHSDRTASFNPIMELRNLDVHYGNFHAVRDVSLDIGRNQIT
                     AFIGPSGCGKSTVLRCLNRTNDLVPGAKVNGRIDYRGHDLYGSEVDPIEVRRRIGMVF
                     QKPNPFPKSIFDNIAYGPRVTGMKLNKTETADLVEHALTRAGLWGEVKDKLDQSGFAL
                     SGGQQQRLCIARAIAVEPDILLMDEPCASLDPIATALIEDLMVELVSDYTVVIVTHNM
                     QQAARVSDRTAFFSAQVDDAGQRFGHLVEVDLTTKMFTNPSDQKTEDYISGRFG"
     gene            complement(6796..7680)
                     /gene="pstA"
                     /locus_tag="AC16Y1_02190"
     CDS             complement(6796..7680)
                     /gene="pstA"
                     /locus_tag="AC16Y1_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007297992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR5831308.1"
                     /translation="MSFDIGVTSKSKNSETNAKSLAFLSLLWFSLFVAFLFLVVLLVT
                     TFLNGIGRYDLRLFTEYNNTTPEDAGARAAILGSVFVVAMTALLAIPIGIAAAIYLEE
                     FADKSKWYNRLIELNIQNLAAVPAILYGMLTLGILTLLGLKNKNVVIGGSLALALLIL
                     PVIILTTREAMRAVPQEIRDGSLALGATQLQTVGRQTLPAAVPGIATGTILAVSRAMG
                     EAAPLLLLGALVFITYDPDGLLAGFTTMPIQIFGWTSAPQEEFKSLAAAAIILLLTIL
                     LSLNALAIFIRNKFAKRW"
     gene            complement(7680..8609)
                     /gene="pstC"
                     /locus_tag="AC16Y1_02195"
     CDS             complement(7680..8609)
                     /gene="pstC"
                     /locus_tag="AC16Y1_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012180687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR5831309.1"
                     /translation="MSATKLSESGFSLKSESKRRGERLIVLTLFLASLISILVTIGIV
                     WSLIVPAIEFFRQVSIVEFLTGTRWAPRFKPATFGVLPLLVGTLWTTTIALLVAVPFG
                     LGAAMYLSEYASEKSRRRLKPILELLAGIPTVVYGFFAIEFVTRVVLKGWLGLDIGTF
                     SVLSAGLVMGVMIIPTIASLSEDALSSVPLAMRQGSMALGANKMQTTIRVVFPAALSG
                     IAAAIVLGISRAVGETMIVALAAGNKPNISLNPLEGAQTMTGFIANAALGDSRVGSLQ
                     YDTLFAVGLLLFVFTLLINFISIRLVRKFRQAY"
     gene            complement(8741..>9457)
                     /locus_tag="AC16Y1_02200"
     CDS             complement(8741..>9457)
                     /locus_tag="AC16Y1_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017882762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR5831310.1"
                     /translation="FKKFCASETDVATASRQIKDEEAADCAAAGVTFTEIIVANDALT
                     VVVNKENDWATCLTVDELKKIWEPAAEGTINSWNQIRSSFPDEPLDLYGAGTDSGTFD
                     YFTKEINGEEGASRTDYNPSEDDNVTVQGVEGAKGALGYFGFSYYEENMDKLNAVQID
                     GGAGCVTPSSATAQDGSYTPLSRPLFIYISNEAAKKAEVLAFAKFFVENDDAITAKAL
                     FVPLSETQKAEAAAAVAKLG"
BASE COUNT         2796 a         2051 c         2008 g         2602 t
ORIGIN      
        1 tttctgatga agtagtttct gaaataaaaa aagtcatcaa tgctgcaaca gtttttgatg
       61 aaatggcacc gctttctgag catgtattga ttcacctaaa tcaaggtggc gatgaattcg
      121 atgaacattt cttagctaaa gatttcactg gtaaaatcat tggctacctg catctggatc
      181 gtactgacga agttgctgga ccaattatcg aagttgtggt gcatccagac tcgcgtaata
      241 acggaatagg cgctcaatta gtaaattatg caacgcagac tattaaagac tctcgaatgc
      301 gactttgggc acatggtgaa ttaggtggag catacgcatt ggcgaaaaaa cttggattcg
      361 ctaaatctcg cgagctttgg caaatgcgca gatcgttgta cgcagaatta ccaaaactgg
      421 aactacccag ttctatccaa attaaaaatt ttttacctga agaaaacatg aaagaattct
      481 taattttaaa ttccattgtt tttgaaaaac atcctgaaca gggtcgatta gacgaaaaag
      541 acttcaaaat taggatgagc gagacgtggt ttcgtagcga aggatttttt cttgcgcaag
      601 atcgggataa aaaattaatc ggatttaatt ggaccaagat acatggccaa gattcggggc
      661 caacaaataa ctacggacat caaaaaatcg gcgaaattta cgttctcgga gttaaacctg
      721 gaaatcggca aagtgggtta ggcaaggcac ttgctattcg tggtctcgaa tatttaagag
      781 aacaaggact cggtgccgcc atgctttatg ttgatcgcac aaatgtgcca gcgataaaac
      841 tgtacgaaag tatcggcttt gctcattggg acactgacgt catgtttcaa aaacaaacta
      901 gctaggtaaa actacttttt cacctgattt gtattgagtt ttatttcctg cgaagaaaac
      961 ccaaacagat ccaacaccct gcacttccca ttcatcgagt ccacctacca gtgcagtatt
     1021 ttcatcaatg ccaacaacgg ttaactcttt gtcatgttta attcgactaa caaaattcat
     1081 aaacatgcga tcaaagtgtg gcaaaactcg gacatttggt aacagaccaa aacctggcgt
     1141 tgtggttcgc ataaaactgc gcaactctgg aatgtgattg gcaattgcca tcgcacctgc
     1201 actacaacca gctaaagctg tcccagccaa ccaagcttca tatattgcgg tccacaactt
     1261 ggtatcccgc aaagtatcag ctaagtaatg tgggttgcct ccagaaaaat aaataagtcc
     1321 ggcaccttct atttgtttta caaattcatc atcgtcagct tttgttcgat cagtgatcat
     1381 taacggtact ggttggactc ctaaacggtt ggcttgggct attcctaagt ctcgccaata
     1441 atttaacgac cgctcacctt cttgcgcggc agcagttgga atctgcacat acttatttcc
     1501 tcgtccagaa attaaatttc cttcgatttg cgccataact ggcagatact cacctgagcc
     1561 aactaaagcg attggacctg gtttcaactg gcatcaatat cccaagttga cccttcggca
     1621 gaatctttaa tggttacttt taacgaagtt agttgatctc gaatttcatc agcttcatcc
     1681 caacgaccac tttgtcgggc agcttgtcta gcgtgtaaaa gcatttcgat gtaaggacca
     1741 accactgaat taatatcaac ccgaggatta gcagccatct gacccaatcg agtgattagt
     1801 ccatgcacga tggctctggt ttctaaatcg cgatctaaat catcaatctc gagtagcgca
     1861 tcaacagctg cgagtacttg tccactttcc aaaagtcttt caacggcatc aggctgagtt
     1921 ggtagttgga ttggcgtgga cgaaacggtg cgcttaaccc cgccttgctc ggctaactct
     1981 gaaatagctg cactttgtcc cgatggcaaa gtccaagtat gttcaccttt tctcaccgtt
     2041 aaattaccgc gaccgaaaac cgttacattt tgtgcatcaa gatcaaattt gattccagta
     2101 tgttcgtcga ccccgagaat aaaagttgtt tcgggtaact gcgcttcgag taaccgaata
     2161 cgacgttctc ccatgtaaca aaatctagta tcgtgcgttc caccttcagc gttatcaaaa
     2221 tgtggaacaa caactgcatc taagccagtg aatttctcta aaatatttaa cccatccaac
     2281 aaataagggt cttcacctgc cttataaatt tcataaaccg gaattgtttt cgttccgatt
     2341 gttagtgctg ctgctgaggc aaaaactaaa gttccttgtt ctaaaacttt ttctagctga
     2401 ttttgtaaac cagtttgtcg ccaaactttt aaggcataac tcggtgaacc tggtccggca
     2461 aatatccatt tagcatttcg taactgattt actgcgttgg cgatggcagc gggtgaatca
     2521 ttcgcttgac gaagctgaac tggttttgga tttaacccga ctgactttgc aaaatagtca
     2581 ctgattcttg aagttaattc atcggcattt tcttgaaaac caaaaggggt atcaagaaaa
     2641 aaagcatatt cttctgtcgt atcggttgcc gaccaaattt gttggtgtgg agtaaccatg
     2701 gttggactgg tttcgccaga acccataatg actatctgtc tactcattag ttaattgttt
     2761 catctaaaca aagtttcctc ttgcccacga ttggcaactg gattcatctg ctagcaaaca
     2821 aggcgaaatc tagagttaac caaattgtgc caatatgaag gcactatgtc ggacttaatg
     2881 gcttcccagc ccccgcaggt aaacctgcca ggtgatcgct atttaaacag agagatgtct
     2941 tggcttgcct tcaaccgacg tgtattagaa cttgcccaag acgaaaatat ggaactactt
     3001 gagcgagtta aatatatttc catttttgct agtaacctcg atgaattttt tatggttaga
     3061 gtggctggct taaagcggcg aattgaaact ggattggcca gaaaagactc gagcggaatg
     3121 caacctcgcg agttgttaga cgaaattttg gctgagagtt acgcactaat gcaggaacac
     3181 gcagcaacat ttaacaactc aattatgcct tctttggtag ctaacggaat tgaaattttg
     3241 cgttgggaag aattaactac tgaagaacgt gaacgaatga gtaatttatt cgctgaacag
     3301 atttttccag ttttgacgcc actcgccgtt gatccttcgc atccttttcc atacatatct
     3361 ggactctcgc ttaacctcgc tatttcagtt aaaaatcctg aaacggaaca tgaacttttt
     3421 gccagagtaa aagtccctgc ttctttgcct cgattttttg aagtatctcg agaacgattt
     3481 gttccacttg aagatgtaat tgctgggcac ctagacatgc tttttcctgg gatggaaatt
     3541 ttgcagcatc acacatttcg agtaactcga aacgaagagc tagaagtaga agaagacgat
     3601 gctgaaaatt tacttcaagc acttgaaaaa gaattgagtc gacgtcgctt tggtgcaccc
     3661 gttcgactgg aagtcgaaaa tactattgat ccacatattc tggagttaat tgttagtgaa
     3721 gttggcgtcg aacaaaatga agtttttaga ataactgggc cacgtgaact tgaatcttta
     3781 gtggaattgg caaaattaaa tcgtgaagat ttaaaggcac cggtgttcgt ttccaaaaca
     3841 agtaccgaac ttgccgaagt tgaatcggca actgcgccaa atgtgatgga agtgatgcgc
     3901 aataaagatg tgttgctaca tcatccgtat gactcatttt caacaagtgt tcaattgttt
     3961 gttcagcagg ctgccgaaga tccaactgtg ctggcgatta aacaaacttt gtatcgaacc
     4021 tccggcgatt caccacttgt taatgcatta attacagcag cacaaaacgg taagcaagtg
     4081 ctagcagttg ttgaaattaa agctagattt gatgagcaaa ataacatcga gtgggccaga
     4141 aaacttgaag aagctggcgt tcatgttgtt tatggaattg tgggattaaa aactcacgca
     4201 aaactatgtc tagtggtgcg taacgacgcc gggaaattaa atcgatacgt ccacatcgga
     4261 actggaaact acaatcctaa aactgcaaga cactacgaag attacggttt actaactacc
     4321 gatccaaaaa ttgctgaaga cgtagcaagc ttatttaatt tattgtctgg ctatggaatt
     4381 caaggtaaat ataaacgtct gattgttgca ccaaaagaat tgcgcaacac gctagtagaa
     4441 aaaattgacc gtgaagcagc taatgcgtgg atcggcctaa cttctggaat cagaatcaaa
     4501 tgtaatgcga tagttgatga aaccatcatg gatgcgcttt atcgagcatc agcagccggg
     4561 gtaaaaattg agattttggt gcgaggtatg tgcgcgttaa agcccggtgt gccaggcctt
     4621 tcggaaaata tttcggtgcg aagtattctg ggtagatttt tagagcacag tagagtttat
     4681 gaattcatga atgctggcga ccgcgaagtt tggattggat ctgccgattt aatgcatcga
     4741 aatctagacc gtcgggttga agcattagta caaataaagc agccaaatgc ttcggccata
     4801 atccaagttc ttgatttggc atttgccaaa accacttctg cttgggagtt aatgccaggt
     4861 ggaaattgga accgacaaaa cttagacgaa gatggtgaat tactcaccga ctatcaggaa
     4921 tttctaatta ataatcaccc agttcccagg tctggaattg atcatccgac tccgcgagta
     4981 tttaatgcat tgttaaataa ggtaggccta ggtgagcgat aaatacataa tcgcagccgg
     5041 cgcagtggtc actcggcaga atgctggtca gactgaatat ttagtaattc atcgtggtta
     5101 ccgtgatgac tggtcttttc ccaaaggaaa agttgatcca ggcgaaaata ttcttggcac
     5161 cgcgattcgg gaagtccgtg aagaaactgg ctatgcaatt aagttaggta tcccgctacc
     5221 aacacaaaag tacaaagtag caaacggaaa taaagagtcg cactattggc acgcaactgt
     5281 gaccggtggc aattttgtgg ccaacgacga agttgaccat ctcgagtggc tctctcttga
     5341 acaagcacaa aaacgtcttt cttatgaaca tgatcatgaa gttttgttag ccgcggctca
     5401 tgcaaaaccc accactccat ttattgtgtt acggcacact caagctatta agcgggctga
     5461 gtgggttgtc tctaaagaag gtttagcaga agtagacgcc agtcgcccgc taactgctgt
     5521 tggtcgaatg caagcagcag gactggttcc ggcacttgct gcttttggaa ttaacgaatt
     5581 gcattcatca gactcaagaa gatgcagaga cactgtaggt ccatatgcat ccgcacgcag
     5641 tatttctatt gagctcgaac caacattaag tgaggagcga cataaagaac atcccgataa
     5701 agcactggat cgggtagcac agttagccca aactaccgaa gcacttgcac tttgtacgca
     5761 ccgaccggtg atgcctacag taatggaatc actcggtggc atttttcaaa ttgaaaatcc
     5821 tggcaaaaaa acttttgatc cagcgttaac tcctggctca atggtgatat tccatcgcga
     5881 gagcgataac ttgatgaatg tacttagcgt cgaacgccac atacattaat ctttatccga
     5941 atcgaccgga aatataatct tcagtttttt gatcacttgg gtttgtgaac atcttggtcg
     6001 ttaggtccac ttcaactaaa tgaccaaagc gttgacctgc atcatcaact tgagcgctaa
     6061 agaatgcagt tcgatcagat acccgagcag cctgctgcat attgtgagta acaataacga
     6121 ctgtgtaatc tgagaccaac tccaccatta aatcttcgat cagcgccgta gcgatcggat
     6181 ctagtgaagc gcaaggctca tccatgagca ggatgtcagg ttctactgca atcgctcggg
     6241 cgatacataa acgttgttgc tgaccaccag aaagggcaaa accactttga tctaatttgt
     6301 cttttacttc gccccaaagt ccagcgcgag taagtgcatg ctcaactaaa tctgcagtct
     6361 ctgttttatt tagcttcatt ccggtaacgc gtggaccgta ggcaatattg tcaaaaattg
     6421 atttggggaa tgggttcggt ttttggaaaa ccattccaat tcgtcgtctg acttcaattg
     6481 gatcaacttc cgaaccgtaa aggtcgtgac ctcgataatc gattcgaccg ttaactttgg
     6541 caccaggtac aagatcgtta gtgcgattta agcagcgcaa tactgtcgat ttaccacaac
     6601 ctgatgggcc aataaatgca gtaatttgat tgcgaccaat atctagcgat acatctctaa
     6661 ctgcatggaa attgccatag tgcacatcta ggttgcgtaa ctccataatt gggttgaaag
     6721 aagcagtgcg gtcactgtgc tttaccggca tttgtcttgc ggtattactg gtctcagtca
     6781 ttttcttctc tcctactacc aacgcttggc aaacttatta cgaatgaaaa ttgcaagggc
     6841 attcagactt agcaaaatgg taagtagcaa aataattgct gccgctgcaa gactcttgaa
     6901 ttcctcttga ggagcactag tccaaccaaa aatttgaatt ggcatcgttg tgaaaccagc
     6961 taataaacca tctggatcat aagtaatgaa taccagagcg cctagcaata gcagtggcgc
     7021 agcttctccc atcgcacgcg aaaccgccag gatagtccca gttgcgatac ccggcaccgc
     7081 ggcaggaagt gtttgacgcc caactgtctg caattgagta gcaccgagcg cgagcgaacc
     7141 atcacgtatt tcttggggca ccgcacgcat cgcttcgcga gtggtcaaaa taatcactgg
     7201 caaaatcaaa agtgctagcg ctagtgatcc accaataaca acatttttat tttttagacc
     7261 aagtaacgtc aaaattccaa gcgttaacat gccgtaaaga atcgccggca ccgctgccaa
     7321 gttttgaatg tttaattcaa ttaatcggtt gtaccacttt gatttatcgg caaactcttc
     7381 tagataaatt gccgctgcga taccaattgg gattgccaat aaggcagtca ttgcaaccac
     7441 gaaaacagaa cctaagattg cggctcgggc accggcatct tctggcgtgg tgttgttgta
     7501 ttcagtaaat aagcgaaggt cgtagcggcc aattccattg aggaatgttg tgaccaatag
     7561 aactactaaa aataaaaatg caacaaaaag actgaaccaa agtaggctaa gaaaagctaa
     7621 agatttagca ttagtttcgc tgttcttact tttacttgtt actccgatat caaatgacat
     7681 tagtaagcct gcctaaattt gcgcaccagg cgaatactga tgaaattaat taacaaagta
     7741 aatacaaaaa gtagcaaacc aacagcgaat aacgtgtcgt actgcaatga gccaacacgc
     7801 gaatcaccaa gcgctgcatt tgcaatgaac ccagtcatag tctgagctcc ttctaatgga
     7861 tttaacgaaa tattcggttt gttaccagca gctagcgcaa cgatcatggt ttcaccgaca
     7921 gcacgagaaa tacctaaaac tatcgctgct gcaatgccag atagcgctgc aggaaataca
     7981 actctgattg ttgtttgcat tttgtttgca cctagtgcca tggaaccttg acgcatagct
     8041 agcggtaccg aggaaagtgc atcttcagaa agggatgcga tcgtcgggat aatcatcaca
     8101 cccatcacta gaccagctga taaaaccgaa aatgttccga tgtctaatcc gagccaacct
     8161 tttagtacga cgcgtgttac aaattcaatt gcgaaaaatc cataaacgac tgttgggatt
     8221 ccagccaata attcaaggat tggttttaat ctgcgacgag atttttctga tgcatattct
     8281 gataaataca tcgcagcgcc taaaccaaaa ggcacagcta ccaaaagagc aattgtggtg
     8341 gtccaaagag ttccaactag taacggcaaa acgccaaaag ttgctggttt aaatcgagga
     8401 gcccagcgcg taccggttaa aaattcaaca atcgaaactt gtctaaagaa ttcgatcgct
     8461 ggaacaatta gtgaccaaac gattccaatg gtgaccagaa tagaaattag cgacgccaaa
     8521 aatagcgtta aaacgattag ccgttcacct ctgcgcttgg attctgattt gagcgagaaa
     8581 ccgctttcgc tcaatttcgt agcgctcatt tagtttggtg ctctttccac gaggattaat
     8641 ttttaagtta tctctataaa caaaagatta gcggcaagga ggtgccgaga tatgggcaac
     8701 tacccaatct cggcacctct tacttatcgt tacttactta ttagcccagt ttggctactg
     8761 cagcagcagc ttcagccttt tgggtttcac ttagcggtac gaataatgcc ttggcagtaa
     8821 tcgcatcgtc attttctaca aagaatttag cgaaagctaa tacttctgct ttcttagctg
     8881 cttcattact aatgtagatg aatagcggac ggctaagtgg ggtgtaactg ccatcttgtg
     8941 ctgtagctga gctcggagta acacagcctg cgccaccatc aatctgcaca gcatttagct
     9001 tgtccatgtt ttcttcgtag taagagaaac caaagtagcc aagtgcacct ttagcgcctt
     9061 cgacaccttg aacagttacg ttgtcatctt ctgatgggtt gtaatcagta cgtgatgcac
     9121 cttcttcacc attaatttct ttggtgaagt aatcgaaggt acccgagtca gttcctgcac
     9181 cataaagatc aagtggttca tctgggaatg aacttctgat ctggttccat gaattaatgg
     9241 ttccttcagc agctggctcc catattttct ttaactcgtc aacagttaga caggtcgccc
     9301 agtcattttc tttgttaaca accacagtta gtgcgtcatt agcaacgatg atttcagtga
     9361 aagttactcc cgctgcagca caatctgcag cttcttcatc tttaatttga cgtgaagcag
     9421 ttgctacatc ggtttcactg gcacaaaact ttttgaa
//
LOCUS       DBNHVN010000117         2342 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1803421, whole genome shotgun sequence.
ACCESSION   DBNHVN010000117 DBNHVN010000000
VERSION     DBNHVN010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1803421"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="AC16Y1_02205"
     CDS             complement(<1..506)
                     /locus_tag="AC16Y1_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5831311.1"
                     /translation="MSVFAELIGQEDAVNQLSRAAQDAQKIVQGQPGSAMTHAWLITG
                     PPGSGRSTSALAFAAALICPQGGCGKCVDCQNALAGTHIDIEHIVPEGVIYTVEDTRT
                     LIERAALAPSRSHWHVIVIEDVDRFRAESASTLLKSLEEPPPGTVWLLCAPTSDDVFE
                     TIRSRCRQV"
     gene            complement(503..1120)
                     /gene="tmk"
                     /locus_tag="AC16Y1_02210"
     CDS             complement(503..1120)
                     /gene="tmk"
                     /locus_tag="AC16Y1_02210"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR5831312.1"
                     /translation="MTGFFIAMEGGEGAGKSTHAKRLAEWLESQGYEVVLTREPGGTP
                     TAEKIRAILLDPEIKDMPDQTEALLFAAARGDHAHNKIKPALDRGAVVICDRYIESSV
                     AYQGIARGLGADQVRELSEWATEQLLPDFTIYMDVPAEDSAQRMDGIDRMEIQSREFH
                     HAVHQAFRDMSANSGKPNILIDATQAKDDVELAIRNAVIAVLPKL"
     gene            complement(1130..>2342)
                     /locus_tag="AC16Y1_02215"
     CDS             complement(1130..>2342)
                     /locus_tag="AC16Y1_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017578818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HIR5831313.1"
                     /translation="EELGIGRPSTYAAIISRIIDQGYVWKKGSALVPHFLAFTVVRLM
                     EENFTQLVDYDFTASLEEVLDQIARGETDRLSVLKRFYFGEQDHGTIFPGLAPLVAVY
                     GDIDARAMASIPIEGHEGIIRVGRYGPYLERGKDETLQRANIPTDLAPDEMTAEKAEE
                     LFNAPSGERELGVDPETGMTILVKTGRFGPYFTEVLPEGSKKSAKAKTASLFSTMDVE
                     SVTLEDALKMLGLPREVGIDPESGEPITAQNGRYGPYLLKGKDSRTLPDENSIFSCTL
                     EEAQALFAQPKIRRGRGQAAGPLKVVGTDPSTGKEVVVRDGRFGTYITDGETNASLRG
                     GDSVETISIERASDLLAERRAAGPSPKKATARKSAAKKATAKKTTTKTVKKSAKKSAA
                     KKTGVGKVAAK"
BASE COUNT          596 a          580 c          508 g          658 t
ORIGIN      
        1 acctgacgac aacggctgcg gatagtttca aaaacgtcat cacttgttgg cgcgcaaagt
       61 aaccagactg tgccaggggg cggttcttct aaactcttaa gtaatgtgga tgcggattca
      121 gctcgaaacc gatcaacatc ctcaatcaca attacatgcc agtgcgaacg gctcggggca
      181 agtgctgctc gttcaattaa tgtacgagta tcttcgacgg tgtaaataac gccttccggc
      241 acaatgtgtt caatgtctat gtgcgtaccc gctaacgcat tctgacaatc aacacattta
      301 ccgcacccac cttgggggca aattaatgca gctgcaaaag ctaatgcgct tgtacttcga
      361 ccacttccgg gtgggccggt aatcaaccaa gcatgtgtca tagcgctgcc cggttggcct
      421 tgaacaattt tttgcgcatc ttgcgcagca cgacttaact ggtttacggc atcttcttgg
      481 ccgataagtt cagcaaagac actcatagtt ttggaagtac cgcaataact gcattacgaa
      541 tcgctaactc aacatcgtct ttagcttgag ttgcgtcaat caaaatattt ggtttgcctg
      601 aattcgctga catatcccgg aatgcctggt gcactgcatg atgaaattcg cgcgattgaa
      661 tttccatccg atcgatgcca tccatacgtt gcgcactatc ttcagccggc acatccatat
      721 aaatagtgaa gtctggaagt aactgttcag ttgcccattc gctgagctcg cgaacttgat
      781 cagcgcctag acctcgtgca ataccttggt aagcaactga agattcaatg tagcgatcac
      841 aaataactac cgcaccgcga tctagggcgg gcttaatttt gttatgcgcg tgatcgccac
      901 gagccgccgc aaaaagtaat gcttcagttt gatcaggcat atctttaatt tctggatcta
      961 acaaaatggc ccgaattttt tctgcagtag gtgtgccgcc tggttcgcga gttaatacaa
     1021 cttcataacc ttgagattcg agccattcgg ctaagcgctt agcgtgggtt gatttacccg
     1081 cgccttcgcc accttccatc gcaataaaga atccggtcat cgcagcacgc tatttcgcag
     1141 cgactttacc gacaccagtt ttcttagcgg cggatttctt agctgacttt ttaacagttt
     1201 ttgtagtggt cttttttgca gttgctttct tggctgcaga tttacgcgca gtcgctttct
     1261 ttggcgatgg gccggcagca cggcgttcag ctagcaagtc agaggcgcgc tcaattgaaa
     1321 tagtttcgac tgaatcgcca ccacgtagcg atgcatttgt ttcaccatcg gtgatgtagg
     1381 taccaaaccg gccatcacga actacaactt ctttaccggt tgatggatcg gtaccaacaa
     1441 ccttaagtgg accagctgct tgaccgcggc cacgacgtat ttttggttgc gcgaataatg
     1501 cttgtgcttc ttcaagtgtg caactaaaga ttgaattttc atctggcaaa gttcttgaat
     1561 ctttaccttt caaaaggtac ggaccataac gaccattttg cgcagtaatc ggctcaccag
     1621 attctggatc tattccaact tcacgcggta acccaagcat tttgagtgca tcttctagtg
     1681 ttacggattc aacatccata gttgaaaaca atgatgcagt ttttgctttt gcagatttct
     1741 ttgatccttc aggcaaaact tcggtgaagt acggaccaaa gcggccagtc tttaccaaaa
     1801 ttgtcatgcc agtttcagga tcaacaccaa gttcacgttc acctgatgga gcgttaaata
     1861 actcttcggc tttttcagcg gtcatctcat ctggcgctaa atctgttgga atatttgcgc
     1921 gctgcaaagt ttcatcttta ccgcgttcta agtacggacc gtatcgtcca actcgaatga
     1981 ttccttcatg accttctatt gggatcgatg ccatagctct ggcatcgatg tcaccgtaga
     2041 ccgctactaa cggagccagc ccagggaaaa tcgtgccatg atcttgttca ccaaaataga
     2101 agcgctttaa tactgacaat cgatctgttt caccgcgagc aatttggtcc aatacttctt
     2161 caagtgaggc agtgaagtca tagtccacta attgagtgaa attttcttcc atcaaccgaa
     2221 ctacggtaaa agctaagaaa tgcggaacta acgcactgcc ttttttccaa acataacctt
     2281 gatcgataat tcgactgata attgccgcat aagttgatgg gcgaccaatt ccaagttctt
     2341 cg
//
LOCUS       DBNHVN010000118         2759 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_837931, whole genome shotgun sequence.
ACCESSION   DBNHVN010000118 DBNHVN010000000
VERSION     DBNHVN010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_837931"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="AC16Y1_02220"
     CDS             complement(<1..264)
                     /locus_tag="AC16Y1_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5831314.1"
                     /translation="MPTKNWKLAAVVLPAALILAACGGTNEGSADPAESTSQIAASEI
                     RIDGSSTVAPLAAAAGELFKEANPEVNVSVGTSGTGGGFKKFCA"
     gene            complement(365..1591)
                     /gene="glgC"
                     /locus_tag="AC16Y1_02225"
     CDS             complement(365..1591)
                     /gene="glgC"
                     /locus_tag="AC16Y1_02225"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006898613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIR5831315.1"
                     /translation="MRRRSNVLSIVLAGGEGKRLMPLTADRAKPAVPFGGSYRLVDFV
                     LSNLVNAGYLRICVLTQYKSHSLDRHITTTWRVGSMLGDYVTPVPAQQRLGPRWYTGS
                     ADAIYQSLNLIYDEMPEYVSVFGADHVYRMDPEQMVAAHIESGASVTVAGIRVPKSQA
                     SSFGIIKTDQSGKNITEFLEKPNDPPSIPDSPNESYASMGNYVFKTEALVEALKKDAA
                     DETSIHDMGSNIIPMLTEKGEANVYDFATNVVPGSTERDKGYWRDVGTIDSYHDAHMD
                     LVSVHPIFNLYNKEWPTLTDLPPLAPAKFVQGGNAHDSIVGAGSIVSGAHVRTSVVAH
                     EVRVNDGAYVDGSVLMPGVQIGRGAVVRKAILDKNVIVPDGAQIGVDLDRDRENYTVT
                     SGGIVVLGKGQQVVTD"
     gene            1626..>2759
                     /gene="glgA"
                     /locus_tag="AC16Y1_02230"
     CDS             1626..>2759
                     /gene="glgA"
                     /locus_tag="AC16Y1_02230"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - alpha-1,4-glucan
                     glucosyltransferase (UDP-glucose donor) activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HIR5831316.1"
                     /translation="MRISIVTKEWPPDIYGGAGVHVEHLVKELQKLAEVDVHAFGEPK
                     SGATTYSTPNELLSANPAVQTLGVNLEMLADLASAQVVHSHTWYANMAGHLGSLLHKI
                     PHVVTAHSLEPLRPWKAEQLGGGYAISSWVEKTSFLSANAVIAVSNGMKTDILNCYPE
                     IDPNRIHVVHNGIDTAAMHKVSTNTALMKYEIDPTRPYVLFVGRITRQKGIMHLLRAA
                     ESLPNEIALVLCASSPDTPELGSEVATQIEKLSKSRTGIHWIKEQVSRPELNEILSNA
                     GVFACPSIYEPLGIVNLEAMACEIPVVASNVGGIPEVVVDEVTGLLVNYDPKNSIAYE
                     VEFAEKLTQVISDQKLASKMGLAGRRRAELEFGWDRIAKETLAV"
BASE COUNT          783 a          631 c          596 g          749 t
ORIGIN      
        1 ggcacaaaac tttttgaagc cgccaccggt accggaagtg cctacagaaa cgttaacttc
       61 aggatttgct tccttaaata gttcaccagc agctgcagct agaggtgcaa ctgttgaaga
      121 gccatcgatg cggatttctg aagcagcaat ctgagaggtt gattctgctg ggtccgcgct
      181 tccttcattt gtgccgccac atgcagcaag gattaatgca gctgggagga caaccgctgc
      241 aagcttccag ttcttagtag gcatttgcct tcctttcatc tgaccatttg gtcaattgaa
      301 aagtaaggtt ttcaagcaaa cacagcccag cgttaaggta aacagaaagt gaatttttct
      361 agatttagtc agtaactact tgctgacctt taccaagaac cacaattccc ccactggtca
      421 cagtgtaatt ttctcgatca cggtctagat caacgccaat ctgtgctcca tctggcacga
      481 tgacgttttt atcaagtatc gcctttcgca ccacagcgcc tcgacctatt tgcacacccg
      541 gcattaaaac tgagccatca acatatgctc catcatttac gcgcacttcg tgtgccacaa
      601 ctgacgtgcg cacatgagca ccactgacaa tcgatccagc accaacgata gagtcatgcg
      661 catttccgcc ctgcacaaat tttgccggag caagtggtgg taggtctgta agtgttggcc
      721 attctttgtt atacaaatta aaaattggat gtaccgaaac taaatccata tgtgcatcat
      781 ggtacgaatc aattgttcct acgtcacgcc agtatccttt atcgcgctca gttgaaccag
      841 gcacaacatt agttgcgaag tcgtaaacat ttgcttcgcc tttttcggta agcattggaa
      901 ttatgttgct acccatgtca tggattgaag tttcatcggc agcatctttt ttcaatgctt
      961 caacaagcgc ctcggttttg aaaacataat tacccatgga tgcataactt tcattgggtg
     1021 aatcaggaat tgacggagga tcatttggct tttctaagaa ttcagttata tttttgcctg
     1081 attgatctgt tttgataatg ccaaaactac tggcttgcga tttaggcact cgaattccag
     1141 cgactgtaac acttgcgccc gattcaatat gagctgcaac catttgctct gggtccatcc
     1201 gataaacgtg atctgcacca aatactgaga catactctgg catttcatcg taaattaaat
     1261 ttagactttg ataaatagca tcagcagaac cggtgtacca ccttggccct aaccgttgtt
     1321 gcgctggtac cggtgttaca taatcaccaa gcattgaacc cacgcgccaa gttgttgtga
     1381 tatgtcggtc aagtgaatgc gacttgtact gagtaagaac gcaaattctt aagtacccag
     1441 catttactaa atttgaaaga acgaaatcaa ctaatcgata tgagccacca aagggcactg
     1501 ctggcttcgc tcgatcagca gttaatggca ttagacgctt accctcgcca ccagcgagca
     1561 caatacttaa tacgtttgag cgcctgcgca tactcacagc ctaatgaaag tcatagagtt
     1621 gtggtgtgcg tatttcaatt gttactaagg aatggccacc agatatttac ggtggtgctg
     1681 gcgtacatgt tgagcattta gtcaaagaat tacaaaaact ggctgaagtg gatgtgcatg
     1741 catttgggga accaaaatcc ggggcaacga cttattcaac tccaaatgag ttattgtctg
     1801 ctaatccagc ggtacaaaca cttggcgtaa acctcgaaat gttggcagat ttagcatctg
     1861 cgcaagttgt gcattcgcat acttggtacg ccaacatggc tggtcatcta ggatctttgc
     1921 tccacaagat tccccacgtt gtcactgcac acagtcttga accactccgg ccctggaaag
     1981 cagagcaact aggtggtggc tacgcgattt catcatgggt tgaaaaaact tcatttctaa
     2041 gtgcaaatgc cgtaattgca gtcagcaatg ggatgaaaac cgacatatta aattgctacc
     2101 cagaaattga tccaaatcgt attcacgtcg ttcataacgg aatagatact gcagcgatgc
     2161 ataaagtttc gactaacact gccttaatga aatatgaaat tgatccaacc agaccctatg
     2221 tacttttcgt tggtcgaatc accagacaaa aaggaataat gcatctactg cgtgctgcag
     2281 aaagtttgcc aaacgaaatc gctttagtgc tctgtgcatc ttcgccagat actccagaac
     2341 ttggtagcga ggttgcaaca caaattgaga aactttctaa atcacgcacc ggtattcact
     2401 ggatcaaaga acaagtaagc cgaccggagt taaacgaaat actttcaaat gctggagttt
     2461 ttgcctgtcc atcaatttat gaaccacttg gcattgtgaa cttagaggcg atggcttgtg
     2521 aaattccggt agttgccagc aatgtagggg gtatacccga agttgttgtc gatgaagtaa
     2581 ccggactgct ggtgaactac gatccaaaaa attcaatagc ttatgaagtt gaatttgccg
     2641 aaaagttaac ccaggtgatt agcgatcaaa agctggctag caaaatgggt ctagcaggtc
     2701 gacgccgtgc tgaacttgag tttggctggg atcgaattgc caaagaaaca cttgcggtt
//
LOCUS       DBNHVN010000119         1048 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1506972, whole genome shotgun sequence.
ACCESSION   DBNHVN010000119 DBNHVN010000000
VERSION     DBNHVN010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1506972"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1048
                     /locus_tag="AC16Y1_02235"
     CDS             <1..>1048
                     /locus_tag="AC16Y1_02235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5831317.1"
                     /translation="IEVIVQIGGVLWDVEVTRPEFDRVSSDLECDLCVVGLGANGLTA
                     ALAAAQRGLNVIAIDADRIAAGAGGRNGGLLLAGIADFHHNARKTFGQQKAAQMYQHT
                     LAEMQLIESTTPSAVKLGGALRLAHDDVELKDCQTHFDALTEDNFPVEWYEGPQGVGL
                     KITSDGVFHPIKRVVELAKLASNAGARIFTHSPATEIHSGEVITPNGKITAKNILVAA
                     DGNLHRIFPELSDKIHPVRLQMIGTAPSKEIQFEYAVYVRDGWDYWQQLPDGRIAIGG
                     GRDMSIETEFTDVNEPTEFMRKYLSSRLLELKVNNKVDYHWSAIVGYTYNDLPIAQEV
                     KQNVYAMVGYCGTGN"
BASE COUNT          305 a          212 c          260 g          271 t
ORIGIN      
        1 atcgaggtaa tcgtgcaaat aggtggcgta ctttgggatg ttgaagttac gcgcccggaa
       61 tttgatcgag tttcgtctga cttggaatgt gatttatgtg tagttggact tggtgctaat
      121 ggtctaactg ctgcacttgc agccgcgcag cgtggtctga atgtaattgc aattgatgct
      181 gaccgcattg cagccggtgc tggcggacgc aatggtggat tgttactcgc cggaattgcg
      241 gattttcatc acaatgcgcg caagaccttt gggcaacaaa aagccgcgca gatgtaccaa
      301 cacacattgg ccgagatgca attgatcgaa agtactacgc catcagccgt aaaacttggt
      361 ggcgcacttc ggttagccca tgacgatgtc gaacttaaag attgtcagac ccacttcgat
      421 gcgctaactg aagataactt tccagtcgaa tggtacgaag gaccgcaagg tgttggttta
      481 aaaataacca gcgacggcgt tttccatcca ataaaacgag tggtggagtt agccaagttg
      541 gctagcaatg caggcgcgcg gatctttacc cactcaccag caaccgaaat acattctggc
      601 gaagttatta cacccaatgg aaaaataact gcaaaaaata ttctggttgc cgctgatgga
      661 aatctacaca gaatatttcc tgaactgagt gacaaaattc atccagtgcg cttacaaatg
      721 atcggaactg cgccaagtaa agagattcag tttgagtatg ccgtttatgt gcgagatggt
      781 tgggattatt ggcagcagct acccgatggc cgaattgcaa ttggtggtgg ccgtgacatg
      841 tcaatcgaaa ctgaattcac agatgtaaat gaaccaactg aatttatgcg taaatattta
      901 agtagccgat tacttgagct aaaagtaaat aacaaagttg attatcactg gtctgcgata
      961 gttggctata cctataacga tttaccaatc gcccaagaag taaaacaaaa tgtttatgca
     1021 atggttggat attgcggtac cggaaatg
//
LOCUS       DBNHVN010000120         1455 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_22147, whole genome shotgun sequence.
ACCESSION   DBNHVN010000120 DBNHVN010000000
VERSION     DBNHVN010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1455)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_22147"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..>1455
                     /locus_tag="AC16Y1_02240"
     CDS             55..>1455
                     /locus_tag="AC16Y1_02240"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIR5831318.1"
                     /translation="MPNVDPKVAGLTLKSGNPLTAVFDAVVVATTTDSKFSIFSDGLT
                     KAAATKLKNTLQAVGATGAKDELIRIPGTGISKSKVIVAVGLGSTKNGVSEEQLRRAA
                     GNATRSLAGFKRVAIYLPATYAGAAKAIFEGAALGAYSFTDHRHATLNKSKSTVSAIS
                     LFTEHAKEKEFKEALDQARTIAHSVHFARDLINTSPLHLPPAEVAKAAKQYLTGTSIK
                     VQILDEKQLASGGYGGILGVGQGSSRPPRLIRMEYKPRVAKKHIALLGKGITFDTGGI
                     SLKPPASMHEMKADMSGAAAVIATIRAIHDLGLPIAVTAYAACAENMPGGGAQRPGDV
                     ITTYGGRTIEVLNTDAEGRLVLADVLVRAAEDNPDLVLDIATLTGAAVVALGHRTAGV
                     MGDDEVRDAVKAAADRAGEEFWPMPLPEELRAPLDSLVADIANIGQREGGMLSAGWFL
                     KEFVEDGLPWAHLDIAG"
BASE COUNT          411 a          331 c          351 g          362 t
ORIGIN      
        1 tagtaacgta gtcattagtc tcaataaggc gacccaaatt aggagtcata accaatgcca
       61 aatgtggatc caaaagttgc cggcttaact cttaagtcag gcaacccgct cactgcagtc
      121 tttgatgccg tagtcgtcgc aacaacaact gatagtaagt tctcaatttt ttctgatggg
      181 ctaactaaag ctgcagcaac taaattgaaa aatactcttc aagcagtcgg tgccactggc
      241 gccaaagatg aattaatccg aattcccggt actggaattt caaaatcaaa agttattgtt
      301 gctgtcggtt taggatcaac aaaaaatggg gtgagcgagg aacaacttcg tcgtgctgcc
      361 ggtaacgcga ccagaagtct ggctggcttt aagcgggttg ctatctattt acctgcgacc
      421 tatgccggcg cggccaaggc catttttgaa ggtgcggcat taggcgcata ttcatttacc
      481 gaccaccgcc atgccacatt aaataagtca aaaagtacag taagtgcgat ttcactattt
      541 acagaacatg ccaaagaaaa agaatttaaa gaagctctag atcaagctcg cacaattgca
      601 cactcggtgc attttgcaag agatttaatc aacacctctc ctttacacct gccacctgca
      661 gaagtagcaa aagcagcgaa acaatatctg accggcacat caatcaaagt tcaaatcttg
      721 gatgaaaagc aacttgcttc tggtggctac ggtggaatac tcggtgtcgg tcaagggtca
      781 agccggccac caagattaat tcgaatggaa tacaaacctc gagttgcaaa aaagcacatt
      841 gcgttactgg gtaaaggaat tacttttgat accggtggca tttcgttaaa gccgcccgca
      901 agtatgcacg aaatgaaagc tgatatgtca ggtgcggcag cagtgatcgc cacaattaga
      961 gcaatccatg atttaggtct accaattgcc gtaactgcat atgctgcatg cgcagaaaat
     1021 atgcccggcg gcggtgcgca acgacccggt gatgtcatca ctacttacgg tggtcgcaca
     1081 attgaagttt taaatactga tgccgaagga cgtcttgtgt tagccgacgt tttagttcga
     1141 gctgccgaag acaacccaga tttagttctt gacatagcca cgttaactgg cgctgctgtt
     1201 gtcgcgctag gtcatcgaac cgctggtgtg atgggtgatg atgaagtgcg agatgcagtc
     1261 aaagccgctg cagatcgcgc tggcgaagaa ttctggccga tgccattacc tgaagagtta
     1321 cgggctccac tagattcttt ggttgcagat attgccaaca ttggtcaacg cgaaggtggc
     1381 atgttatctg ctggctggtt cctcaaagag ttcgtagagg atggtctacc ttgggcccat
     1441 ctagacattg ccggt
//
LOCUS       DBNHVN010000121         1544 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1359242, whole genome shotgun sequence.
ACCESSION   DBNHVN010000121 DBNHVN010000000
VERSION     DBNHVN010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1359242"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="AC16Y1_02245"
     CDS             <1..152
                     /locus_tag="AC16Y1_02245"
                     /inference="COORDINATES: protein motif:HMM:NF015113.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HIR5831319.1"
                     /translation="NADRSGAKFALIMGEEELVANSAVVKNLSNGAQESVAVDLVAQH
                     LLNNL"
     gene            174..>1544
                     /gene="aspS"
                     /locus_tag="AC16Y1_02250"
     CDS             174..>1544
                     /gene="aspS"
                     /locus_tag="AC16Y1_02250"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR5831320.1"
                     /translation="MLRTHDAGKLRKTDAGLEVQLTGWVSRRRDHGGIAFIDLRDRSG
                     TIQVVINDESAANELRNEFCLKIVGQVRIRPEGNENTEIPTGDIEVVAENIEVLSKAA
                     PLPFPIDDSINVGDETRFKYRYLDLRRDSQAQALRMRSEVNRLAREVLHERDFTEIET
                     PTLTRSTPEGARDFLVPVRLQPGHWYALPQSPQLFKQLLMVAGMERYYQIARCYRDED
                     FRKDRQPEFTQLDIEMSFVEQEDIIEVGEAIVRNLWRGTVGHEIAEIARITYFEAMDK
                     YGSDKPDLRFNLELRDCTEYFANTEFRVFQNEYVGAVVMPGGADQPRRTFDAWQEWAK
                     QRGAKGLAYVTFELDGTLGGPVAKNLSESERSGLAKHLQANPGDCAFFGAGPRLTTQQ
                     LLGAVRQEVGSRCGLIDGNAWSFLWVVDAPMFELTDEGNWTAVHHPFTSPNGDWINNF
                     EQNPGEA"
BASE COUNT          444 a          323 c          393 g          384 t
ORIGIN      
        1 aaaatgccga cagaagtggt gcaaaatttg ccctaataat gggtgaggaa gagttggttg
       61 ccaattctgc agtggttaag aatttgtcca acggagcaca agagtctgtc gcagtagact
      121 tagtcgcgca acatctttta aataatttat aagtacttct taccgaggaa taaatgttac
      181 gaacgcatga tgcgggcaaa ctccgcaaaa ccgatgctgg tctcgaggtc caactcactg
      241 gttgggtgag tcgtcggcgc gaccacggag gtatcgcatt tattgatctt cgcgaccggt
      301 ctggaacgat tcaagttgta ataaatgatg aatcggcggc taacgaatta cgcaatgaat
      361 tttgcctgaa gatagtcggt caagtaagga tacgacccga aggtaacgaa aatactgaga
      421 taccaactgg cgatatcgag gtagtagccg agaacatcga agtactgagt aaggcagcgc
      481 cacttccttt ccccattgac gattcgataa atgtcggtga tgaaacaaga tttaagtatc
      541 gctacctaga tttgcgcaga gattcccaag cgcaagctct gaggatgcga agtgaagtca
      601 atcgacttgc tcgcgaggtt ttacatgaac gcgatttcac tgagatagag actccgacgt
      661 taacaagatc aactcctgaa ggagcgcgcg atttcctagt tccagtccgg ctgcaacctg
      721 gacattggta tgcactgcca caaagtccac aactttttaa gcaactactc atggtggccg
      781 gcatggagcg ctactaccaa attgccagat gttatcgcga tgaagatttt cgaaaggatc
      841 gtcaacctga gttcacccag ctagacatcg agatgagttt tgttgaacaa gaggacatta
      901 tcgaagttgg cgaagccata gtaaggaatc tttggcgggg gacagttgga catgaaatcg
      961 ctgaaatagc acggattacc tattttgagg ccatggataa atacggttca gataaaccgg
     1021 acttgcgttt taatttagag ctgcgcgact gcacagaata ttttgcaaat accgaattta
     1081 gagtctttca aaatgaatac gtaggggctg tagtaatgcc tggcggtgcc gatcaaccga
     1141 ggcggacttt tgatgcctgg caagaatggg ccaagcaacg tggtgccaaa ggtttggctt
     1201 atgtcacttt tgagctagac ggaacgctcg gcggtccagt tgccaagaat ttatctgagt
     1261 cagaacgttc tggattagca aaacatcttc aggccaatcc tggcgactgc gcattttttg
     1321 gtgcgggtcc cagacttaca actcaacaat tactcggcgc tgttcgccaa gaagtgggaa
     1381 gtcgttgtgg attaatagat ggcaatgctt ggtcattctt atgggtagta gacgcaccaa
     1441 tgtttgaact aacagatgaa ggtaactgga cggccgtcca tcatccattc acatcaccaa
     1501 acggtgattg gataaataat ttcgaacaaa atccgggtga agct
//
LOCUS       DBNHVN010000122         1271 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1062972, whole genome shotgun sequence.
ACCESSION   DBNHVN010000122 DBNHVN010000000
VERSION     DBNHVN010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1062972"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="AC16Y1_02255"
     CDS             <1..200
                     /locus_tag="AC16Y1_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010064158.1"
                     /note="chaperone Hsp40; co-chaperone with DnaK;
                     Participates actively in the response to hyperosmotic and
                     heat shock by preventing the aggregation of
                     stress-denatured proteins and by disaggregating proteins,
                     also in an autonomous, dnaK-independent fashion; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR5831321.1"
                     /translation="PRLRGSGRGDLIIHLEVQTPTDLTKEQEDLLNQLAKARGEEKPA
                     SAFSEQSSGLFGKLKDAFSGR"
     gene            197..928
                     /locus_tag="AC16Y1_02260"
     CDS             197..928
                     /locus_tag="AC16Y1_02260"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIR5831322.1"
                     /translation="MSAQVYLTPAGTLPEGINAFELTGDEANHAVKVKRTEIGELIEI
                     VDGQGVRICGTVSQVTKKSLTLDVKTRIVESKPDLTLTVAQAIAKGDRADLALELLTE
                     VGVDYILPWKAEFAIAKWEDQAKGKSKWQATVAQASKQSRRSWIPLVHDLVSGEKLAT
                     LAKQFDVAVVLHESAKQSFAQIPLPQSGSVLLIVGPEGGISPKELEQFKELSQVRMGK
                     TVMRTSTAGAIAAGAILSKTRWIGE"
     gene            931..>1271
                     /locus_tag="AC16Y1_02265"
     CDS             931..>1271
                     /locus_tag="AC16Y1_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIR5831323.1"
                     /translation="MSDCLFCKISAKEIPADIVLETQSVLAFRDIDPKAPTHILVIPK
                     MHFENAGELANSDKELAVQVLQATVDVAMLEGLAEGYRIVSNTGSDGGQSVNHVHFHV
                     LGGRSLNWPPG"
BASE COUNT          387 a          246 c          314 g          324 t
ORIGIN      
        1 tgccaaggtt gcgaggcagt ggccgcggcg atttgataat tcatcttgaa gtgcaaacgc
       61 caactgactt aactaaagaa caagaagacc tgctaaatca actagcaaag gctcgtggtg
      121 aagaaaagcc agcctctgca tttagcgagc agagttcggg actatttgga aaattaaagg
      181 atgcgtttag cggtcgatga gtgcccaggt ctatttgact cctgctggaa ctttgcctga
      241 aggaataaat gcttttgaat taactggtga tgaagcaaat cacgcagtaa aagttaaacg
      301 tactgaaatt ggcgaactca tcgaaatagt cgatggtcaa ggggttcgaa tttgcggcac
      361 cgtttcacaa gtaacaaaaa aatcgttaac actagatgtc aaaactcgaa tcgtggaatc
      421 aaaacctgat ttgacgttaa ctgttgctca agcaattgca aaaggcgatc gagccgattt
      481 ggcacttgag ctattgactg aagtgggtgt cgattacatt ttgccctgga aagcggagtt
      541 tgctatcgca aaatgggaag atcaggctaa aggaaaaagt aaatggcaag cgactgtcgc
      601 acaggcttca aaacaatcta gacgaagttg gattccgctt gttcatgact tagttagcgg
      661 tgaaaagtta gcaaccttag ctaaacaatt tgatgtcgca gtagttttac acgaaagtgc
      721 aaaacaaagt tttgctcaaa tccccttacc gcaaagtgga tctgtacttt taatagttgg
      781 tcccgaaggc ggaatatcac ctaaagaact tgagcaattc aaagaactta gtcaggttcg
      841 gatgggtaaa acggttatgc gcacttcaac cgctggtgcg atcgctgccg gtgcaatact
      901 tagtaaaact cggtggatcg gagagtaacc atgagtgact gtttattttg caaaattagt
      961 gccaaggaaa ttcccgcaga tattgtttta gaaacacaat cagttttggc tttcagggat
     1021 atagatccaa aggcaccaac gcatatattg gtcattccga agatgcattt cgaaaatgct
     1081 ggagaattag caaattccga taaggaattg gctgtccagg tccttcaagc aacggtggat
     1141 gtagcaatgc ttgagggcct tgctgagggg tatcgaattg tttcaaatac tggcagcgat
     1201 ggtggccagt cagtgaatca cgtacatttc cacgtactcg gtggccgaag cctaaattgg
     1261 cctcccgggt a
//
LOCUS       DBNHVN010000123         2624 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_393931, whole genome shotgun sequence.
ACCESSION   DBNHVN010000123 DBNHVN010000000
VERSION     DBNHVN010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_393931"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="AC16Y1_02270"
     CDS             complement(<1..213)
                     /locus_tag="AC16Y1_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HIR5831324.1"
                     /translation="MSIQSVFPQLEQLLPRVQKPVQYVGGELNAVVKPWDDVLVRWCL
                     MYPDAYEVGIPNQGVQILYEVINERAD"
     gene            complement(222..629)
                     /gene="ndk"
                     /locus_tag="AC16Y1_02275"
     CDS             complement(222..629)
                     /gene="ndk"
                     /locus_tag="AC16Y1_02275"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIR5831325.1"
                     /translation="MSERTLVLVKPDGVERGLVGEVLSRIERKGFKLVALELRTLDRA
                     TAQTHYGEHKDKPFFKDLVDFITSGPLLAAVIEGNEAIAAWRTMMGATNPANAAMGTI
                     RGDLATETQFNVSHGSDSPESAAREIKLFFPNL"
     gene            complement(650..961)
                     /locus_tag="AC16Y1_02280"
     CDS             complement(650..961)
                     /locus_tag="AC16Y1_02280"
                     /inference="COORDINATES: protein motif:HMM:NF025383.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4233 domain-containing protein"
                     /protein_id="HIR5831326.1"
                     /translation="MKVMGSATMVFQAIVIGLTIPVAVANYGVPVSTAIWLATIFIII
                     CLLAVGGIRRDRRTAIITGSTVQVLVLLSAFWVKPMLFPGIVFTAIWLLAIKLSAKTP
                     A"
     gene            complement(964..2328)
                     /locus_tag="AC16Y1_02285"
     CDS             complement(964..2328)
                     /locus_tag="AC16Y1_02285"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010838294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR5831327.1"
                     /translation="MDWDEPQEISIGHLRRVEEVLESRQPETIIEPSLDRIKHLMDLL
                     GDPHLAYPVIHITGTNGKTSTSRMIEILLREMGLNTGLITSPHLHQITERIRLNGQPI
                     EPERFVNTYDEIEPFLALVDTQSLATGGPAMSYFEVLTGMAFAAFADAPVDVAVIEVG
                     MGGTWDATNVVKPAVSVVTPIGMDHAEYLGDTVEKIAVEKSGIIKSDSIAILSPQIPS
                     VAEILLNRTAEVGAQVARQGVEFSVRDRALAVGGQLLTFDGLNGTYEQIILPLFGQHQ
                     AANASLALAAVEAFFGGITKLSEDSVRRGFANATSPGRLEVVKRSPTVIVDAAHNPHG
                     AQVLANALQESFTFDSIIGVISVMADKDVLGVLEALEPVLQEVVVTWNGAARAMSVEE
                     INKLAISVFGADRVHTRPTLSSAIDYAIELADNIATSGVGVVITGSVVTAARGRALTG
                     HIEA"
     gene            2372..>2624
                     /locus_tag="AC16Y1_02290"
     CDS             2372..>2624
                     /locus_tag="AC16Y1_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR5831328.1"
                     /translation="MTTDRMPEKPTLDGLETKWANTWENQGTYSFDRSATRENIFSID
                     TPPPTVSGSLHVGHVFSYTHTDLIARYQRMIGKKVFYPMG"
BASE COUNT          725 a          636 c          551 g          712 t
ORIGIN      
        1 gtctgcacgt tcattgatta cttcataaag aatttgcacg ccttgattgg gaattccaac
       61 ctcgtaggca tctgggtaca tcaagcacca gcgaaccagc acgtcatccc aaggtttaac
      121 aactgcattt agttcaccac caacatattg aactggtttt tgaactctag gcagtaactg
      181 ttccagttgc ggaaatacgg actggatact cataaaacct cttaaagatt cgggaagaaa
      241 agttttattt cacgagccgc tgattcaggt gaatcagaac catgtgaaac attgaattgg
      301 gtttcggtgg ctaggtcgcc acggatagtt cccattgctg catttgctgg attagttgca
      361 cccatcatgg ttcgccaggc tgcaatggct tcattacctt cgattacggc tgcaagcaac
      421 ggcccacttg tgatgaaatc gactaagtct ttgaaaaatg gtttatcttt atgctcaccg
      481 taatgtgttt gtgctgttgc tcgatccaaa gtgcgaagtt caagtgcgac taacttgaag
      541 cctttgcgtt cgatccgact taagacttcg ccgactaatc cgcgctcgac accatcgggt
      601 ttaactaata caagtgtgcg ttctgacatg tctttaattc tagacgggat taagcaggtg
      661 tctttgcgct caatttgatg gccagtagcc agatggcagt gaagacgatt ccaggaaaaa
      721 gcattggctt tacccaaaat gcgctcagta gcactaaaac ttgcacagtt gagccagtaa
      781 ttattgctgt tcggcgatct cgacgaatac cacccacagc caacaaacaa atgattataa
      841 atattgtggc aagccaaatc gctgtactaa ctggaacgcc ataattggca accgcaactg
      901 gaatagttaa tccgattacg atggcttgaa aaaccatggt tgcgctgccc attactttca
      961 tttttatgcc tcgatgtgtc cggttaatgc gcgaccccgc gcagctgtaa caactgaccc
     1021 ggtgattaca acaccaactc cagaagttgc aatgttatct gccagttcta tcgcgtaatc
     1081 aattgctgaa ctgagcgttg gccgagtatg cacgcgatct gcgccaaaaa cagaaatagc
     1141 aagtttgtta atttcttcaa ctgacatggc tcgtgctgca ccattccagg taacgaccac
     1201 ttcttgcaaa acaggttcca acgcttcaag tacgcctaag acgtctttat ctgccatcac
     1261 cgaaataacg ccgataatcg agtcaaaagt gaatgactct tgtagagcgt ttgcaagtac
     1321 ttgagcaccg tgcggattat gggccgcatc gactatgact gttgggctgc gtttaacaac
     1381 ttctaagcgg ccaggtgaag ttgcatttgc aaaaccacgt cgcaccgaat cttcgcttaa
     1441 cttagttatg ccaccaaaaa atgcctcaac tgcggcgagt gcaagtgatg catttgctgc
     1501 ttggtgttgt ccaaatagtg gcaagataat ttgttcgtac gttccattta gtccatcgaa
     1561 agttagcaac tgcccaccaa ctgcgagcgc tcgatcccgg acagaaaatt caacaccttg
     1621 ccgtgccact tgggcaccaa cttctgcagt ccggttcaac aaaatttctg ccacactcgg
     1681 aatttgcggt gacaaaattg cgatcgaatc tgatttgata attccagatt tttcaacggc
     1741 aattttttct acagtgtcgc caagatattc cgcatgatcc atgccaattg gtgtaaccac
     1801 actcaccgca ggtttaacta catttgtagc atcccacgta ccacccatgc caacttcgat
     1861 aaccgcgacg tcaaccggag catcagcaaa agctgcaaag gccataccgg ttaaaacttc
     1921 aaagtaactc atcgctgggc caccagtggc cagactctgc gtatctacca aagcaaggaa
     1981 cggctcaatt tcgtcataag tatttacaaa acgctctggt tcaattggct gcccatttaa
     2041 tcgaatgcgc tcagtaattt gatgtaaatg cggacttgtg attaatccag tatttaagcc
     2101 catctcgcgc aataagattt caatcattct cgatgtagat gtcttgccgt ttgtgccagt
     2161 gatgtgaatt actgggtaag ccaaatgggg atcgccaagt aaatccatca gatgtttaat
     2221 gcgatccaga cttggttcaa taatcgtctc gggttgccgt gactcaagaa cttcttcaac
     2281 gcgcctaagg tggcctatcg aaatttcctg tggctcatcc caatccatgg gttaattcta
     2341 ggttccatga aacctcgtag aattatggct catgaccact gatcggatgc cagaaaaacc
     2401 cacactcgat gggttagaaa ccaagtgggc aaatacttgg gaaaaccaag gcacatactc
     2461 atttgatcgc agtgctactc gagaaaacat attctcaatc gataccccac caccaacagt
     2521 ttcaggatct ttacacgttg gtcatgtttt ttcctatact cacactgatt taattgctcg
     2581 ttatcaacgc atgattggca aaaaagtttt ttatccaatg ggtt
//
LOCUS       DBNHVN010000124         1219 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_615163, whole genome shotgun sequence.
ACCESSION   DBNHVN010000124 DBNHVN010000000
VERSION     DBNHVN010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_615163"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="AC16Y1_02295"
     CDS             complement(<1..1025)
                     /locus_tag="AC16Y1_02295"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF015418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR5831329.1"
                     /translation="MSETIFDAPLDVAMDIAYNVAPPQEIIELPISDVAGHVLALDIV
                     AKSALPPFAASRVDGYAISGTGSWRLIGENLAGQATELELTSGQCCYVATGAPVPKNT
                     LAVIKQEDCVLVDSVVALKPDMGEIAAGDNIRAAGFESLENEVVIKASTKLIPALLGL
                     VSACGYDLISVYRKPTVDVFIFGDELIRQGFASAGKVRDSIGPQVSAWLEFFGGQLNE
                     IQYIPDRLEQHVRAIKNSTADLVITTGGTASGPADHLHKAIEQCAGEILIDAVNVRPG
                     YHQLLAQLPNKFLIGLPGNPQSAVIGLFTLVWPFITGSTNQTKRVWELRRLATSVSAP
                     VNEHKFVV"
     gene            1088..>1219
                     /locus_tag="AC16Y1_02300"
     CDS             1088..>1219
                     /locus_tag="AC16Y1_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014448.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HIR5831330.1"
                     /translation="MATIVETTVTTEVLDADYFRNLVSDPTAGAVVIFAGDVRNHDGG
                     "
BASE COUNT          343 a          274 c          272 g          330 t
ORIGIN      
        1 acaacgaatt tatgttcgtt tactggagca gaaaccgagg ttgccaacct gcgtagctcc
       61 catactcgct tcgtctggtt agtactgccg gtgataaatg gccaaactaa agtgaacaat
      121 ccaattacgg cagactgcgg gttaccgggc aatccaatta ggaacttatt gggaagttga
      181 gcgagtaact gatggtagcc aggccggaca tttactgcat caatcaagat ttcacccgca
      241 cattgctcaa tcgccttgtg taaatggtct gctgggccac ttgcagttcc accagttgta
      301 ataaccaaat ctgcagttga attttttatt gcgcgaacat gttgctctaa ccgatctgga
      361 atgtactgga tttcatttaa ttgaccacca aaaaattcaa gccacgcaga aacttgaggt
      421 ccaattgaat ctcgcacctt gccagccgat gcaaatccct gacgaatcaa ttcatcacca
      481 aaaataaaaa catccacagt tggttttcga taaacggaaa tcaggtcgta gccacacgca
      541 gaaactaggc caagtagggc tgggattaat tttgtgcttg ctttgattac gacttcattt
      601 tcaagagact caaagccagc tgctctgata ttgtctccgg cagcaatttc gcccatatcg
      661 ggtttgagtg caactacaga atcgacaagt acacaatctt cttgcttgat tactgcgagg
      721 gtattttttg gtaccggggc accggtggca acgtaacaac actgtccaga agttagttca
      781 agttctgttg cttgtccggc taagttttct cctattaatc tccatgatcc agtacctgag
      841 atcgcataac cgtcaacgcg ggatgcggca aatggcggta atgcggattt agccacaatg
      901 tcgagcgcta atacgtgacc ggcaacatca gaaatgggca gttcaattat ttcttgtggc
      961 ggtgccacat tgtatgcaat gtccatcgcc acatcaagtg gcgcatcaaa aatagtttcg
     1021 gacatggact tattctgcct tatcacccag ttcaactgat gaaagcaagg taaacaataa
     1081 ttaacttatg gcgacaatag tcgaaacaac ggtaaccaca gaagttttag atgctgatta
     1141 ttttcgcaat ttagtaagtg atccaacggc tggtgcagtg gtcatttttg ctggcgatgt
     1201 tcgaaatcat gatggggga
//
LOCUS       DBNHVN010000125         2522 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_615350, whole genome shotgun sequence.
ACCESSION   DBNHVN010000125 DBNHVN010000000
VERSION     DBNHVN010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_615350"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..1630
                     /gene="purH"
                     /locus_tag="AC16Y1_02305"
     CDS             71..1630
                     /gene="purH"
                     /locus_tag="AC16Y1_02305"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005238563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR5831331.1"
                     /translation="MSDQIKIRRALISVSEKTGLIELAAGLNESGVEIVSTGSTAQTI
                     SQAGIPVIEVKEVTGFVECLDGRVKTLHPNIHAGLLADLTRPEHVVQLEELGITPFEL
                     VIVNLYPFNKTVQSGASVSECIEQIDIGGPALVRAAAKNHLNVGVVVEPSYYQELLYA
                     VKQGGLTLNQRKAFAVKAFTHVAEYDVSVATWLTQNLDANETEFPEWIGKVWHRQTQL
                     RYGENPHQQAAVYKSAQDNLGLASAKQLHGKEMSFNNYVDADAARRAAYEHEQPAVAI
                     IKHANPCGIAIGQDISLAYSAAHATDPVSAFGGVIAANREVTADMATSVMEVFTEVIV
                     APSYEKAALEILMKKDNLRILQVANVPNEITVESRHISGGLLVQSEDKFQADDADPKN
                     WKLVSGNPADEETLKDLVFAWRASRCVKSNGILLAKSGGAVGIGMGQVNRVDSARLAV
                     SRAGVNRAENSVAASDAFFPFADGLQVLIDAGVKAIVQPGGSMRDGEVIAAAVNAGIT
                     MYFTDTRHFFH"
     gene            1627..>2522
                     /locus_tag="AC16Y1_02310"
     CDS             1627..>2522
                     /locus_tag="AC16Y1_02310"
                     /inference="COORDINATES: protein motif:HMM:NF016535.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5831332.1"
                     /translation="MSKTVAKQKVNIWHLLIGLLVATFNSSQARVNGELGAMLDNAIV
                     AALVSFGSGLTILLIGVIFIRPARKAYFGIPNLIRNQKLHWWQIIGGTAGALFVIGQG
                     FVVPVVGVAIFVISVVAGQTCASLLVDKFGLGPAGPKPITLLRVFSAGLAVVGVIIAV
                     LGRANTGTFNLPMVIYMFTVGALTSAQHAMNGRVAQETKQPWETTTLNFTVGMSVLLV
                     LFLLYNLITDVAIPIPPAPWEQPLLWTGGAIGIIFIVSAVVLVRTLGVLIFALVSVVG
                     QLLGAVLLDIFFPTPGNTVTFQV"
BASE COUNT          704 a          472 c          600 g          746 t
ORIGIN      
        1 aaagaattgc tcgttcaagt ggtcaagcag tttgtttcta aagaactgct aactaaggca
       61 ggaaagtaaa ttgtctgatc aaataaaaat tagacgggca ttaataagtg tttctgaaaa
      121 aactgggctc attgaattag ctgctggact aaatgaatca ggtgttgaaa tagtttcaac
      181 tggcagtact gcgcaaacca tttcgcaggc tggaattcca gttatcgaag ttaaagaagt
      241 taccggattt gttgaatgcc tagacggtcg agttaaaacc ttacatccaa atatccatgc
      301 tggtttatta gccgatttaa ctaggccaga acacgtagtt caacttgaag aattaggtat
      361 tacgccattt gaacttgtga tcgtaaattt gtatcccttc aataaaactg tgcagtctgg
      421 cgcaagtgtt agtgagtgca tagagcaaat tgacataggc ggaccggcac tagttagagc
      481 tgccgctaaa aatcatttaa atgttggtgt tgttgttgaa cccagctatt accaagaatt
      541 actttacgca gttaagcaag gcggattgac tttaaatcaa cggaaagcct ttgcagtcaa
      601 ggcttttacg cacgttgctg agtacgacgt ttcggtcgct acttggttga cgcaaaatct
      661 tgatgcaaat gaaactgaat ttccagaatg gattggcaaa gtttggcatc ggcaaacaca
      721 gttgagatat ggcgaaaatc cacatcaaca agcggctgtt tataaatcgg cacaagataa
      781 tctcggttta gctagcgcta aacagcttca cggtaaagaa atgtccttca ataattacgt
      841 ggatgccgat gctgcgcgcc gggccgctta tgaacatgag caacctgcag tcgcgattat
      901 taaacatgca aatccctgcg gaattgcaat cgggcaggac atcagccttg cttactctgc
      961 ggcgcatgcc acagatccag tttcagcatt cggaggagtt attgccgcaa atcgagaagt
     1021 tactgcggac atggcaacct cagtaatgga agtgtttacc gaagtgattg ttgcgccaag
     1081 ttatgaaaaa gctgcgctgg aaattttaat gaaaaaagat aatctacgaa ttttgcaagt
     1141 tgccaatgtg ccaaatgaaa tcacagtcga atctagacat atttcaggtg gacttctggt
     1201 acaaagtgaa gataaatttc aggccgatga tgctgatcca aaaaattgga aacttgtttc
     1261 aggtaacccg gcagacgaag aaactttaaa agatttagta tttgcttggc gagctagccg
     1321 ttgcgttaag tcaaatggaa ttttattagc caaatctggt ggcgcagttg gaattggaat
     1381 gggccaggtt aatcgagttg atagtgccag acttgcggtg agtagagcgg gtgtaaatcg
     1441 ggcagaaaat agtgtggctg cgtcagatgc attctttcct tttgctgatg gactacaagt
     1501 attgattgat gctggagtta aagcaatagt gcaaccgggt ggatccatgc gagatggcga
     1561 agttatagct gctgctgtaa atgctggcat aacaatgtat ttcactgata cgaggcattt
     1621 cttccattga gtaaaactgt tgcgaaacaa aaagtaaata tttggcatct gcttattggc
     1681 ttactagttg ccacatttaa tagttcgcaa gcgcgagtaa atggtgaatt gggtgcgatg
     1741 ttagacaatg caattgtcgc tgcactagtt tcatttggtt ccggactaac tattttgtta
     1801 attggcgtca tatttattag acctgctcgc aaagcatatt ttggaattcc taatttgatt
     1861 cgaaaccaaa aattacattg gtggcaaata attggcggaa ctgctggcgc attgtttgta
     1921 atcggacagg gtttcgttgt tccagtggtt ggcgtagcaa tcttcgtaat ttcggttgtt
     1981 gctggtcaaa cgtgtgcttc attgctagtt gacaaatttg gtttgggtcc ggcagggcca
     2041 aaacctatta cgttactgcg tgttttttcg gctggtttag ctgtggttgg tgtgattatt
     2101 gctgtacttg gtcgagctaa tactggaacg tttaatttac cgatggttat ttacatgttc
     2161 acggtaggtg cactcacctc cgctcaacat gcgatgaatg gccgagtggc ccaagaaaca
     2221 aaacagccgt gggagaccac aacgctaaat ttcaccgtcg gtatgtccgt acttttggtt
     2281 ctatttttgc tttacaactt aattacagat gtagccatcc caattccacc agctccgtgg
     2341 gaacaaccgc ttctttggac gggcggtgca attggaatta tttttatagt ttcggcagtt
     2401 gtcttggttc gaactctggg agttctaatt tttgctttag tttcagtcgt cggacaactt
     2461 ttaggtgctg tattactaga catctttttc ccaacaccag gcaacactgt gacttttcaa
     2521 gt
//
LOCUS       DBNHVN010000126         6277 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1064267, whole genome shotgun sequence.
ACCESSION   DBNHVN010000126 DBNHVN010000000
VERSION     DBNHVN010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6277)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1064267"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="AC16Y1_02315"
     CDS             <1..375
                     /locus_tag="AC16Y1_02315"
                     /inference="COORDINATES: protein motif:HMM:NF020253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HIR5831333.1"
                     /translation="GMDWIVSKKKDFIGKRSFSRSDTSRAGRHQLVGFLPLDGTEVIP
                     EGAYVTAESDLLTKPPVAHIGYVTSSYMSAALGRSFAMGMLANGQARHGEQVAIPFDG
                     KVVMATVTEPIFYDKENTRRDG"
     gene            368..931
                     /locus_tag="AC16Y1_02320"
     CDS             368..931
                     /locus_tag="AC16Y1_02320"
                     /EC_number="1.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF016180.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:1901053 - obsolete sarcosine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma"
                     /protein_id="HIR5831334.1"
                     /translation="MVNVLNGAKSPLNGAKHGNENSVLLQEVPFTPMWDLRVDPNSAG
                     FGAVNTALGLPLPTSVGDVTKNGDGTINALTLGPDQWMLTGSVDVQAQLASVRSAHHM
                     SLVDVSAQRTKIEIFGPNAKEVLEHVWEQDLREQNFGLDKCSQGIMFRSPVIMWHCCP
                     DCYLIFVRSSFAQHLWAALTDATVEYL"
     gene            975..1823
                     /locus_tag="AC16Y1_02325"
     CDS             975..1823
                     /locus_tag="AC16Y1_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014909936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HIR5831335.1"
                     /translation="MSAVILDGKATAAAVKADLAKRVQVLKARGITPGLGTILVGDDP
                     GSHAYVGGKHKDCHEVGIASIREDLPANATQADVLAAISRLNQDPACTGYIVQLPLPK
                     GLDSNAALEAMLPSKDADGLHPTNLGRLVLGEPAPLPCTPRGIVELLRAYDVNIDGAE
                     VCIVGRGITVGRPLGLLLTRRSENATVTLCHTGTKDMAAHIKRADIVVAAAGVPHFIK
                     PDMIKPGAALLDVGITRTDQGLLGDLDPACASVAGFIAPMPGGTGPMTRAMLLANVVD
                     TAEGQK"
     gene            1823..3472
                     /gene="betA"
                     /locus_tag="AC16Y1_02330"
     CDS             1823..3472
                     /gene="betA"
                     /locus_tag="AC16Y1_02330"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HIR5831336.1"
                     /translation="MTKVYDYIVIGGGSAGSVLGNRLSEDPNNEVLVLEAGRKDSIWD
                     VYIHMPAALTFPIGNKFYDWKYETDPEPFMNNRRVYQGRGKVLGGSGSINGMIFQRGN
                     PMDYERWAGDKGMEKWDFAHCLPYFKKMENATGVPNDKVYRGHSGPLYLEKGPAKNPL
                     FKAFFDAAVQAGFTRTDDVNGFKQEGFGPFDKNVKKGRRWSSARAYLHPVKHRKNLTI
                     KTRHQVNKVIFEGDKAVGVEVRIGKKVVEYRGHEMILCGGAINTPQVLQLSGIGPAQL
                     LNKHGIPVVKDLPGVGQNLQDHLEVYVQHACKQPITMNPNLKVWKRPFIGAAWLFARK
                     GPGATNHFEGGGFARSNTSVKYPNLMYHFLPIAVRYDGTGIEQGHGYQVHVGPMYSDA
                     RGRVEIVSTDPKAKPSILFNYLSTEQDRREWVEAIQVTRKILAQSAFAPFDDGELSPG
                     PSVQTPEQILEWVRKDGETAYHPSCTAKMGKDEMSVVDPETMKVHGVEGLRVVDASVF
                     PYVTNANLYAPVMMVAEKAADIILGKEPLAPEYVEWYKSDK"
     gene            3536..4069
                     /locus_tag="AC16Y1_02335"
     CDS             3536..4069
                     /locus_tag="AC16Y1_02335"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIR5831337.1"
                     /translation="MPRKVLVLLSHPYLESSNVHRNLSQRLAQIENVTVRHIDKLPTK
                     NTHFDAKTEQPFWDEADTIILEFPMYWYHSPASLKQYLDDVLTEDWAYQNAYSLEGKK
                     LTVLTTTGGVESEYAASGEHGFDLSSVLTPYKSTASFTKMKYVPELVIYAANFLTEAE
                     LESEINRIVQAVTALTI"
     gene            complement(4070..>6277)
                     /locus_tag="AC16Y1_02340"
     CDS             complement(4070..>6277)
                     /locus_tag="AC16Y1_02340"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HIR5831338.1"
                     /translation="VVDLLWQTDEIRIERPEVLDEARNALYYLDEIMRTSLGHVLEDL
                     DSALSSLGVKLPANSRPLSLGSWIGGDRDGNPFVTPQVTLDVIGLQRSHAISDLIPLL
                     SRTIEDLSISQKIAGENKQIQEFVAKSIKEVAGLHARYLRVNAEEPWRLALTAVRQKL
                     FNTKDRLDNNDEHVAGSDYATTDEFLGDLELIRTSLLEVGNEDLAVRVIDRLIAVASS
                     VGLHLAALDVREHSARFQDVIAQLVDVADPSCNYRDKTPAEKLIYLQKELASNRPLAA
                     SPPPLNSDGLHVFGAYTAIKQAHELYGSTVCETAIVSMTRGADDLLAAVVVGREAGLV
                     DLNRNYVAVDFVPLLETVEELKNADRILAELLSTPEYRKIVEFRGNVQEVMLGYSDSN
                     KDAGILTSQWEIHLAQRRLRDVATKHGVRLRLFHGRGGTVGRGGGPTFEAIMAQPFGV
                     LTGELKVTEQGEVISDKYILPPLARENLALTLAAVLEASVLHQKPVVPEEKMNHWNLV
                     MQHASDSAFNTYRELVNHPDLPAYFEKSTPVRELADVHMGSRPARRPDTSSGISGLRA
                     IPWVFGWTQSRQIVPGWFGVGSGLKAAREAGFENELAEMVKNWTFFANFISNVEMTLV
                     KTDLTVAQNYVDALVPESLQSLFTTIKAEYELTKAEILKLTGAQTLLESNPSLSRTLA
                     IRDTYLLPLHHLQITLLNRVRTSRDQGQEPDAQLRRALSITVNGIATGLRNTG"
BASE COUNT         1733 a         1391 c         1426 g         1727 t
ORIGIN      
        1 ggtatggact ggattgtttc caagaagaaa gatttcattg gtaagcgttc attctcgcgc
       61 agcgatacgt ccagagctgg tcgtcatcaa ctagttgggt tcttgccttt agatggaact
      121 gaagttattc cagaaggcgc gtacgtaact gcagaaagtg atttattaac taagccccca
      181 gttgcccaca ttggatatgt aaccagttca tacatgtcag cggcgctcgg ccgatctttt
      241 gccatgggta tgttggcaaa tggtcaggca cgacacggtg agcaagttgc gattccattc
      301 gacggcaaag ttgtgatggc aacagttact gaaccaattt tctatgacaa ggagaacact
      361 cgtcgcgatg gttaacgtat taaacggtgc taagagtccg cttaacggag ctaagcacgg
      421 aaatgaaaat tcagtgttgc tgcaggaagt tccatttacg ccaatgtggg acttgagagt
      481 tgatccaaat tctgctggct tcggtgcagt aaatactgca cttggcttac ctctgccaac
      541 aagtgtcggt gatgttacaa aaaatggtga cggaactatc aatgctttga cgcttgggcc
      601 tgatcaatgg atgcttactg gttcagttga tgtgcaggca caactcgctt cagttagatc
      661 tgctcaccac atgtcgctag ttgacgttag tgcacagcgt acaaagattg aaatctttgg
      721 tccaaatgca aaagaagttc tagagcacgt ttgggaacag gaccttcgcg aacaaaactt
      781 tggcctagat aaatgttcac aaggcatcat gttccgaagt ccggtcataa tgtggcactg
      841 ctgccctgac tgttacttaa tctttgttcg gtcttctttc gcacagcact tatgggctgc
      901 gctaactgat gcaacggttg agtatcttta gataagcaag ttttactaaa acatttcgta
      961 ctaggagtgc cagcatgtcc gcagtaattc tcgatggcaa agcaactgct gctgcagtta
     1021 aagctgactt agccaaaaga gttcaagtat tgaaagctcg cggtatcacc ccgggtctag
     1081 gcacaatctt ggtgggtgac gacccaggtt cacatgctta cgtcggtggc aaacataaag
     1141 attgtcacga agttggcatt gcctcaattc gcgaggatct tccagcaaac gcaactcaag
     1201 ctgatgtact tgctgcgata agtcgcctaa atcaagatcc ggcttgtacc ggctacattg
     1261 tgcagcttcc actgccaaaa ggtttagatt caaatgccgc tctcgaggca atgttgccaa
     1321 gtaaagatgc tgatggctta catccaacta atcttggtcg gctagttctt ggtgaacctg
     1381 cccctcttcc atgtacgccg cgcggaatag tcgaattact tcgggcttac gatgtgaaca
     1441 ttgatggcgc tgaagtttgt atcgtcggcc gtggaataac agttggccgc ccacttggtt
     1501 tattacttac tcggcgaagt gaaaatgcaa ctgtcacact ttgccatact ggaactaaag
     1561 atatggctgc tcacattaaa cgcgcggaca ttgtggtcgc tgctgcaggt gttccgcact
     1621 ttattaagcc ggacatgatt aaacctggtg ctgccctact tgatgttggc atcacccgca
     1681 ctgatcaagg gctacttggt gatttagatc cggcctgcgc tagcgttgcc ggttttattg
     1741 caccgatgcc ggggggcact ggaccgatga ccagagccat gttgctagca aacgtggttg
     1801 atacagctga agggcaaaaa taatgacaaa agtttatgac tacattgtta ttggcggtgg
     1861 atcggctggt tcagtattgg gtaatcgtct ttccgaagat ccaaataacg aagtgttagt
     1921 tcttgaagct ggccgtaaag attcaatttg ggatgtctac atccacatgc cagcagcact
     1981 tacatttcca atcggaaata agttttatga ctggaagtat gaaacagatc ctgagccatt
     2041 tatgaacaac cgtcgcgtct atcaaggtcg tggcaaagtt cttggtggct ccggctcaat
     2101 taacggcatg atttttcagc gcggaaatcc aatggactac gaacgttggg cgggcgacaa
     2161 aggaatggag aaatgggatt ttgctcactg tcttccttac tttaagaaaa tggaaaatgc
     2221 tactggcgta ccaaacgata aggtttaccg aggacacagc ggaccacttt atttagaaaa
     2281 aggtccagca aagaatccgc ttttcaaagc attcttcgat gcagcagtgc aggcaggctt
     2341 tacccgaacc gacgatgtga atgggtttaa gcaagaaggt tttggtcctt tcgataagaa
     2401 tgtaaagaaa ggccgtcgtt ggtcttcagc tcgggcgtac ctgcatccag ttaagcaccg
     2461 caaaaactta acaatcaaaa cgcgccatca agtaaataag gtaattttcg aaggcgataa
     2521 agcagttggt gtcgaggttc gcattggcaa gaaagtagtt gaataccgcg gtcacgaaat
     2581 gattttgtgc ggcggtgcga ttaatacccc acaagtattg caactttccg gaattggacc
     2641 ggcgcaatta ctgaataaac acggtattcc tgtagttaaa gacttacctg gtgtgggcca
     2701 aaatttgcag gatcatcttg aggtttatgt gcaacacgca tgtaagcaac caatcaccat
     2761 gaatccaaac ttaaaagtat ggaaacgtcc atttattgga gcggcttggt tgtttgcccg
     2821 taagggtcct ggtgccacaa atcactttga aggtggtggt ttcgctaggt caaatacaag
     2881 cgttaagtac ccaaatttga tgtaccactt ccttcctatt gcggttcgct atgacggcac
     2941 cgggattgaa caaggtcatg gttatcaggt tcacgttggc ccaatgtatt cagatgcgcg
     3001 tggtcgagta gaaattgtga gcacggatcc aaaagctaag ccatcaattt tgtttaacta
     3061 cttatcgact gaacaagatc gacgtgaatg ggttgaagca attcaagtta ctcgcaaaat
     3121 tctggctcag tcagcatttg ccccatttga tgatggtgaa ttaagcccag gaccgtctgt
     3181 gcaaactcct gaacagatct tggaatgggt tcgcaaagat ggcgaaactg cttatcaccc
     3241 atcttgcaca gcgaaaatgg gtaaagacga aatgagtgtt gtcgatcctg aaactatgaa
     3301 agttcatggt gttgagggat tacgcgtcgt ggatgcttcc gttttcccgt acgtcactaa
     3361 tgcaaatctg tatgcgccag taatgatggt ggcagagaaa gcagcagaca taattttagg
     3421 taaagagcca ttagcgcctg aatatgttga atggtacaaa tcagacaagt aatacttgtt
     3481 cactaaaggc aggtctcgag tttcataact ttgggtctgc ctttagtctt agatggtgcc
     3541 aagaaaagta ttagtgctgt taagtcatcc ctaccttgaa tcatcaaatg ttcatcgcaa
     3601 tttatcacag cggttagcgc aaattgaaaa tgtaactgtt cgccatattg ataaattgcc
     3661 tactaaaaac acacactttg atgccaaaac tgaacaacct ttttgggatg aagccgacac
     3721 aattattttg gaatttccaa tgtattggta tcacagccca gcatctttaa agcagtacct
     3781 agatgatgtg cttactgaag attgggcgta ccaaaatgcc tattcactcg agggcaaaaa
     3841 actaacggtc ctaaccacta caggtggggt cgaatctgaa tacgctgcca gtggtgagca
     3901 tggatttgat ttatcttcgg tactaactcc atacaaatca acggctagtt ttaccaaaat
     3961 gaaatacgtt ccagaattgg taatttatgc agcaaatttc cttactgagg ctgaactaga
     4021 aagtgaaatt aaccgcatag ttcaagcagt tactgcatta acaatttgat caaccagtat
     4081 tacgaagtcc agttgcgatt ccattaacgg taatactcaa cgcccgacgc agttgcgcat
     4141 caggttcttg gccctggtcg cgacttgttc gtacccggtt tagcaaagtg atttgcaaat
     4201 gatggagcgg caatagatag gtatcccgaa tagccaacgt tctgcttaat gatggattac
     4261 tttcaagtaa cgtttgagcg ccggtcaatt tcaaaatttc ggctttagtt aattcatact
     4321 ctgcttttat ggtggtgaat aaagattgca aagactctgg gacgagtgcg tcaacgtaat
     4381 tttgcgccac cgttaagtca gtttttacca gtgtcatctc aacatttgaa ataaagtttg
     4441 caaagaaagt ccagttttta accatttcag caagttcatt ttcaaaacct gcttctcttg
     4501 cagcttttag gccactaccg acaccgaacc aacctggaac aatttgtcgc gattgcgtcc
     4561 agccaaatac ccacggaatt gcccgaagcc cagatatgcc cgaacttgta tcaggtctac
     4621 gagctgggcg cgagcccata tgtacatctg ctaactctct taccggagtt gatttttcaa
     4681 agtatgcagg tagatccgga tggttaacta attctcgata agtattgaat gcagaatctg
     4741 aagcgtgttg cattaccaaa ttccagtgat tcattttttc ttcaggcaca actggctttt
     4801 ggtgcaaaac tgatgcttcc aaaacagcag caagcgttag ggctaaattc tctctggcaa
     4861 gtggaggcaa aatatattta tctgaaatga cttcaccttg ttcagtcact tttaattcac
     4921 cggtaagcac cccaaacggt tgggccataa ttgcctcgaa tgttggtccc ccgcctcgac
     4981 cgactgtgcc accacgaccg tggaataaac gcaaccgtac tccgtgctta gtagcgacat
     5041 cacgtagtcg acgttgggct agatgaattt cccattgcga agtcaaaata cccgcatctt
     5101 tattcgagtc tgaatagcca agcattactt cttgtacatt gccgcgaaat tcaacaattt
     5161 ttcgatactc cggagtggag agtagctccg ccaaaattcg atctgcattt ttcaattctt
     5221 caactgtttc tagcagcggc acaaaatcaa ctgcgacata atttctgttt aaatcgacta
     5281 acccagcttc acggccaacg actaccgctg ccaataaatc gtcggctccc ctggtcatcg
     5341 agacaatcgc ggtttcgcaa actgttgagc cataaagttc atgcgcttgt ttaattgcgg
     5401 tgtaggcacc aaatacatgt agcccatcgg aattaagcgg cggtggactc gcagccaacg
     5461 gccgatttga ggctaattct ttttgtaaat agatcagttt ctctgccgga gttttatcgc
     5521 gatagttaca tgatggatca gcaacatcta ctagttgggc aattacgtct tggaatctcg
     5581 ctgaatgctc tcgaacatct aacgcggcta gatgtaaacc gactgaactt gcaaccgcaa
     5641 ttaggcgatc aataacccgc acagctaaat cttcattacc aacttcaagt aaagaagtcc
     5701 tgattaactc taaatcacct aaaaactcat ctgttgtagc gtaatcactg cctgcaacat
     5761 gttcgtcgtt gttatctagt cgatctttgg tgttgaacaa cttttgtctt accgccgtca
     5821 aagccaatcg ccatggctct tctgcattta cccgtaaata ccgagcatgt aatccagcta
     5881 cttctttgat tgatttggca acaaattcct ggatttgttt attctcgccg gcaatttttt
     5941 ggctaattga tagatcttcg attgtgcgcg aaagaagtgg tatcaaatca gagattgcgt
     6001 gactgcgctg tagtccaatt acatccaaag tgacctgcgg ggtaacgaat gggtttccat
     6061 cccgatcgcc tcctatccaa ctccccaaag aaagtggacg agagtttgcc ggaagtttca
     6121 ccccgagact ggaaagagca ctatccaaat cttcaagcac gtggcctagt gaagttcgca
     6181 taatttcatc gaggtaataa agcgcatttc tagcttcatc taaaacttcg gggcgttcaa
     6241 ttcttatttc atcagtttgc caaagtaaat caacaac
//
LOCUS       DBNHVN010000127         3915 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1881338, whole genome shotgun sequence.
ACCESSION   DBNHVN010000127 DBNHVN010000000
VERSION     DBNHVN010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1881338"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1622)
                     /locus_tag="AC16Y1_02345"
     CDS             complement(<1..1622)
                     /locus_tag="AC16Y1_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR5831339.1"
                     /translation="MQIAQLVQDNFGIAGEVSQLPGERDLNYKITSMQDAYIFKIHDA
                     AEREFIELQQKILNLVSEVSKLDQPTPIPAKSGSTIIDLPDGKIGRLLTWADGNLFSQ
                     NEKTTELKQSLGVAVALVDKKLAEFDVAPYLAVLTRPFGWNALQAAGLTEKINLISDE
                     HLRVEVKKTFEFAINQILPNLTKLTNQLIHNDANDNNIVVAQNSVNSLIDFGDVIYAP
                     KICGLAVACAYAINDLADPILDVLPVIRGYHLTNPLTQEELALLFGLIKLRVATSIVM
                     AAIQSAAQPENEYLLISQDWFNQLIKSLNQTDPNLAWYRIRNSCGYEANPNSRLIRQY
                     LQSGRATIADVVMPPLKEVKKIWLDWSSDNPEIPRETAQIFNLMNESGAQLAIGHYCE
                     NRLVYQGEAFSIDSPARRSVHLGVDLFAPAGTAVFAALDGVVEYFNDNNVYLDYGPVI
                     ILRHQTDTGIPFFTLYGHLSRPSLENLYLGKKISAGDLIATMGEEHENVGWPPHTHFQ
                     LLTDLCGMGLDIYGVAPLLDLPVWRSISPNPN"
     gene            1699..2100
                     /locus_tag="AC16Y1_02350"
     CDS             1699..2100
                     /locus_tag="AC16Y1_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831340.1"
                     /translation="MRNSRYRYTRLVMALDSVGTFISGALLLVIPDTSLVFLGFGTTE
                     IEIWFARFIGLLSLTYGILLSTTSRHVEDKYFQRATWALIGVNAISAMSIYGAPGDMT
                     TGRESSTVLFALVTFLFLVTLPIKPIGYKEK"
     gene            2109..3311
                     /locus_tag="AC16Y1_02355"
     CDS             2109..3311
                     /locus_tag="AC16Y1_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR5831341.1"
                     /translation="MSVLDEFDLQNPDLVINPYPAFAELRKTKELLWSDRLGIWLAPT
                     YELANATLRTRTLGRIYQDRLPQNEWEVFNWLHSDSILDSEPPKHTRLRSLVQKAFTP
                     GRIESMRPNVEQLCTQLLDDAQDKLSTQGQFDVLADYAEPLPVLVIADLLGVPRDVAP
                     DLRRWSQDIVKMYEFSKTREQELAAQKAGFEFSQYISELAKDRKQNPQNDLITALVEV
                     EEQGEKLNEHELIAMCVLLLNAGHEASVNGFGNGMVALFNHPEQLSALSANPRELSAT
                     ALEEMMRFDSPLHLFERTATADTQIGSITVKSGEKVGAMLGSAHRDDEVFANAQSFDI
                     KRTPNSHIAFGAGIHFCIGAPLARLEMNTSLPMLFERFPKLKLAGQPEQRPTFVLRGY
                     ETIPVTVD"
     gene            complement(3315..>3915)
                     /locus_tag="AC16Y1_02360"
     CDS             complement(3315..>3915)
                     /locus_tag="AC16Y1_02360"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HIR5831342.1"
                     /translation="TPELLVKRNADTVTSRVLAGTVRRSSDENRDYELAAGLLGSDKD
                     LEEHEYAVNSVAAALASHCIDLDVPTKPFILQLANVQHLGTDITGQLVDSAPVLALAA
                     SLHPTAAVCGTPTERAAGLIKELENMDRGRYSAPVGWMNKSGDGEMGIALRCAAIEDK
                     AKTRLRLFAGCGIVAGSTSEAEVAESNSKFSAMRNALDS"
BASE COUNT         1105 a          846 c          822 g         1142 t
ORIGIN      
        1 aagtttggat ttggtgaaat acttcgccaa acaggtaagt caagtaatgg ggctacacca
       61 tagatatcta gccccatccc acacagatca gttaataatt gaaagtgcgt gtgcggtggc
      121 cagccaacat tttcgtgttc ttcacccatg gtggcaatca gatcaccagc agatattttt
      181 ttaccaagat aaagattttc aagtgatggt ctagaaaggt gcccgtataa agtgaaaaat
      241 ggaattccag tatcagtctg atggcgcaaa ataattactg gaccataatc caaataaaca
      301 ttgttgtcgt tgaagtactc aacaacgcca tcaagtgcag caaatactgc agtacctgca
      361 ggggcaaata aatcaacacc tagatgaacg ctgcgccgag ctggactatc tatcgaaaaa
      421 gcttcacctt gataaaccaa gcgattttcg cagtagtgac ctattgcaag ttgggcacct
      481 gattcgttca ttaaattgaa aatctgcgct gtctcgcgtg ggatttctgg attgtcgctt
      541 gaccagtcaa gccaaatctt ttttacttct ttcagtggtg gcataacgac atctgcgata
      601 gttgcgcgac cactttgtaa atactgtcga attaaacggc tatttgggtt agcttcatag
      661 ccacagctat ttcggattcg ataccaagcc aaattcgggt cagtctggtt gagcgatttg
      721 atcagctggt taaaccaatc ctggcttatt aataggtact cattttctgg ttgagcggca
      781 ctttggatcg cagccatcac gatacttgta gcaacgcgca acttaattaa tccaaaaagc
      841 agcgcaagct cttcttgggt taatggatta gttaagtgat aaccgcgaat tactggaagc
      901 acatccaaaa ttggatcagc taggtcatta attgcgtatg cgcaagcaac tgccaagcca
      961 caaatctttg gtgcataaat tacatcacca aaatcaatta acgaattaac tgagttttgc
     1021 gcaaccacaa tgttgttgtc atttgcgtca ttgtgaatta attggttggt tagtttagtt
     1081 agattaggaa ggatttgatt aattgcaaat tcgaaagttt tcttaacttc gacccgaaga
     1141 tgttcatctg aaattagatt tattttttca gttagaccag ctgcttgaag tgcgttccaa
     1201 ccaaatggtc gagttaacac tgctagatag ggagccacat caaattcagc taacttttta
     1261 tcgacaagtg caaccgccac accaagtgat tgtttgagct ctgttgtttt ttcgttttgg
     1321 gaaaataaat ttccgtcggc ccaagtcagt aagcgaccaa ttttcccgtc aggtaaatca
     1381 ataattgtgc ttccagactt agctggaatt ggagttggct gatcgagctt tgacacttcg
     1441 ctcactaaat tcaaaatttt ctgttgaagc tcgatgaatt cccgctcagc agcgtcatga
     1501 attttaaata tgtaagcatc ttgcattgaa gtaattttgt agttcagatc gcgttcacca
     1561 ggcagttgag atacttcgcc ggcgatcccg aagttatcct gaacaagttg cgcaatctgc
     1621 atacttttat gttgccagat tggtttaaat acccgcccag cgccgtcatc ttttgagttt
     1681 gtatctacac ttacgagtat gcgtaactct cgttatcggt atacccggct agtcatggct
     1741 ttggattcag tcggaacgtt catttctggc gccctacttt tagtaatccc cgatacgtca
     1801 ctggtattcc tcgggtttgg cacaaccgaa atagagattt ggtttgccag atttattggt
     1861 ttactttcgc taacttatgg aatcttgctc tcgactacgt caaggcatgt tgaagataaa
     1921 tattttcagc gggcaacttg ggcgctcatt ggtgttaatg cgatatcagc gatgtcaatt
     1981 tatggtgcac ctggtgacat gacaactggc agagaatcaa gcacagtctt gtttgctcta
     2041 gtgacctttt tattcttagt cactttgccg atcaaaccaa ttggatacaa agaaaaataa
     2101 aaccagtcat gagtgtttta gacgaattcg atttacaaaa cccagactta gttattaatc
     2161 cttatccggc attcgccgaa ctacgtaaga ccaaagagtt actttggagt gaccgattag
     2221 gcatttggtt agcaccaact tatgaattag ctaatgcaac tttgcgcaca agaacgcttg
     2281 ggcggattta tcaagatcga ctcccgcaaa atgaatggga agtttttaat tggttgcact
     2341 cagattcaat tttggatagc gagcctccga agcacacccg gcttcgttca ctagttcaaa
     2401 aagcatttac gccagggcgg attgaatcaa tgcgccccaa tgttgagcaa ctttgcaccc
     2461 agctattgga tgatgcgcaa gacaaacttt ctacccaagg ccaattcgat gttctagcag
     2521 attatgcaga accgctaccc gttttggtaa ttgcggatct attaggcgta cctcgagatg
     2581 tggcacctga tttacggcgt tggtcacagg acattgtaaa aatgtatgag ttttcaaaaa
     2641 cgcgtgaaca agaattagcc gcacaaaaag ccggttttga atttagccaa tacatttccg
     2701 agcttgctaa agatcgaaaa caaaatccgc aaaacgattt gattacagct ttagtggaag
     2761 ttgaagaaca aggtgaaaaa ttaaacgaac atgagttaat cgccatgtgc gtgctattgc
     2821 taaacgctgg tcatgaagca tcggtcaatg gttttggaaa tggaatggtt gcgcttttta
     2881 atcatccaga gcaactatcc gcactaagtg ccaatcctag agaattgagc gccaccgcat
     2941 tagaagaaat gatgcgattt gattcaccac tccatttatt cgagcgcacc gccacggctg
     3001 acactcaaat tggatctata actgttaaat ctggtgaaaa agttggcgcg atgcttggga
     3061 gtgcacatcg cgatgatgaa gtatttgcta acgcgcaatc atttgacatt aaacgaacac
     3121 caaatagtca tattgcattt ggagctggga ttcacttctg cattggcgct ccgctagcca
     3181 gacttgaaat gaatacttca ttaccaatgt tgtttgaacg atttcccaaa ttaaaactcg
     3241 ctggccagcc agagcagcgc ccaacatttg tgcttagagg ttacgaaact attccagtaa
     3301 cagttgatta gaaattaact gtccagtgca tttcgcattg cactaaattt tgaatttgat
     3361 tcagctactt cagcttcgct ggtcgaacca gcaacaattc cacaaccggc aaataaccgc
     3421 aatctagttt ttgccttatc ctcgatagcc gcgcaacgta gtgcgattcc catttcgcca
     3481 tcgccagatt tattcatcca gccaactggc gctgaataac gtccacgatc catgttctct
     3541 aattctttga ttaaaccggc agcgcgttca gttggggttc cacatactgc agctgttgga
     3601 tgtagcgatg cagctaaagc taaaactggt gcactgtcta ctaattgacc agtgatgtca
     3661 gttcctaaat gctgcacatt cgctaattgc aaaataaatg gtttagttgg tacgtctaag
     3721 tcaatacagt gcgaagctag tgctgctgcg accgaattca ctgcgtattc atgttcttcc
     3781 aaatctttat cgctaccaag taacccagca gctaactcgt aatctctgtt ttcatcacta
     3841 ctgcgccgaa cagttccggc aagtactcga cttgttacgg tatcggcgtt tcgttttact
     3901 aaaagttcag gtgtt
//
LOCUS       DBNHVN010000128         1281 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_395526, whole genome shotgun sequence.
ACCESSION   DBNHVN010000128 DBNHVN010000000
VERSION     DBNHVN010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_395526"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="AC16Y1_02365"
     CDS             <1..707
                     /locus_tag="AC16Y1_02365"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HIR5831343.1"
                     /translation="ENSVAGPRSLEHLVDTVLTFEGDRSTPLRLLRANKNRYGPADEV
                     ACFEQADDGLREVSDPSSLFRSQRDQAVPGTCITITMEGRRALLVEVQALTTSSPANN
                     PIRRTSGLDNSRAAMLIAVTERAGRLKLWDKDVFLSTVAGAKISDPGADLGMCLAIAS
                     AAQDKALPIDLVAIGEVALSGDIRPVPGINQRLSEAARLGFATALVPRGTKLPPNVKD
                     LKVIQVDNLTSALKVF"
     gene            762..>1281
                     /gene="disA"
                     /locus_tag="AC16Y1_02370"
     CDS             762..>1281
                     /gene="disA"
                     /locus_tag="AC16Y1_02370"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HIR5831344.1"
                     /translation="MAIREKSDSDSNVLAVLAQVAPGTALRDGLERILRGSTGALIVL
                     GQDKVINQISSGGFVVNTDFTATRLRELAKMDGAVLCDRDATRIYSAAVQLLPDPSIP
                     TDEQGTRHRTADRVSRQTHLPVISVSKSMNIIALYLDGKRYVLEDAAAILSKANQALA
                     TLERYKARLDEVE"
BASE COUNT          354 a          287 c          300 g          340 t
ORIGIN      
        1 gagaaaactc agttgccgga ccacgatctc tggaacattt agttgacacc gtgttaactt
       61 tcgagggtga tcgaagtact ccactgcggt tacttcgagc aaataaaaac cgttatggcc
      121 cagccgatga agtagcttgt tttgaacaag ccgatgacgg tttacgcgaa gtaagtgacc
      181 cgtcatcttt atttaggtcg caacgagatc aagcagtacc aggaacctgc atcacaatca
      241 ctatggaagg tcgccgggct ttattagttg aagtgcaggc gttaactact tcaagtccag
      301 caaataatcc aattagacga acatctggtc tggataattc tcgagctgca atgttgattg
      361 cggtaacaga gcgagcgggc cggctaaagc tttgggataa agatgttttt ctatctactg
      421 tcgcgggcgc aaaaatctca gatccaggtg ctgaccttgg gatgtgttta gcaatcgcca
      481 gcgctgccca agataaagct ttaccaattg acctcgttgc tatcggcgaa gtcgcactaa
      541 gtggtgacat tcgcccagtc ccaggcataa atcagcgttt gagtgaggcg gcccgactag
      601 gttttgctac agcactcgta cctcgcggaa caaaactgcc gccgaatgta aaagacttaa
      661 aagttatcca ggttgataat ttaacttctg cgcttaaagt tttttaacta cctggtttat
      721 ccaatagact cttgctaact tcacgcaaga aagtgcttta agtggcaatt agagagaagt
      781 ctgattcaga ctcaaatgtt cttgcggttc ttgctcaagt tgccccaggt actgcacttc
      841 gtgatggttt agaacgaata cttcgtggtt caactggtgc actaattgtt ctaggtcaag
      901 acaaagttat taatcagatc agctcaggtg ggtttgttgt taataccgac ttcaccgcaa
      961 cacgtctacg cgaactagca aaaatggacg gtgctgtact ttgtgaccgt gatgctaccc
     1021 gcatttattc tgccgccgtc cagttattac cggatccaag tattccaacc gatgaacaag
     1081 gtacgcgcca ccgaactgca gatcgcgtat cgcgtcaaac acatttacca gtaatcagtg
     1141 tgagtaagtc gatgaatatc atcgcgcttt acttagatgg caaacgatac gtacttgaag
     1201 atgcagcggc gattttgtct aaggctaatc aagcgctagc aactttagaa agatacaaag
     1261 cgcgtcttga tgaagttgaa g
//
LOCUS       DBNHVN010000129         1309 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_247806, whole genome shotgun sequence.
ACCESSION   DBNHVN010000129 DBNHVN010000000
VERSION     DBNHVN010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_247806"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1127
                     /locus_tag="AC16Y1_02375"
     CDS             <1..1127
                     /locus_tag="AC16Y1_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5831345.1"
                     /translation="YQDGLILDRGFQLYNPSYEEGFHILDLKLLNLKSLTAGILISID
                     GRQYKLGDPRKEPSWAVDSMLAPVGSIPSKLKFAKYAIKNAFAKNKKSQFDQRTDQFL
                     TRQFGRDLTQKALRPFLAGVFLEDELATSKRFFDIVLRSFVKGVPAVPENGMFEIPKQ
                     LAAQLPIGSIHLNTTVTNISPGIVQTTEGSISCRAIILAANARSAATLIPSLKVPPSN
                     SVTTWYHLADCAPQDLTEGAGTLIVDGKRYSSHAIDPNRPVVNTVAISNAAPNYSGGN
                     RVLVSASTTGVHHTMAGESAVRKHLATLYKVSTTTWQHVGTYPIPDALPAMLPPFNLA
                     KSPRLGDGVYVAGDYRDVSSINGAFRSGRIAAEAAIADSF"
     gene            complement(1124..>1309)
                     /locus_tag="AC16Y1_02380"
     CDS             complement(1124..>1309)
                     /locus_tag="AC16Y1_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HIR5831346.1"
                     /translation="LVQAQTLPLSFATDDHAGIVYHGIDPVEVVIDKADQSARAYRVE
                     KINSEVVETVLPVGKLS"
BASE COUNT          384 a          287 c          278 g          360 t
ORIGIN      
        1 attaccaaga tggcttaatc cttgatcgtg gattccaact ttataacccc tcttacgaag
       61 aagggttcca catcttggac ttaaaattac taaacctaaa atcactaact gcaggcatcc
      121 taatttcgat tgatggacgc caatataaat taggtgatcc ccgaaaagag ccatcttggg
      181 ccgttgattc aatgctggcc cctgtcggat caatcccaag caaacttaaa tttgccaagt
      241 atgcaataaa aaatgcgttc gcgaaaaata aaaaaagcca atttgatcag cgcactgatc
      301 aattcttaac tcgtcaattt ggaagagatt taacacaaaa agcgcttcga ccttttttag
      361 ctggtgtttt tctagaggat gaacttgcaa catctaaaag atttttcgac atcgtgttaa
      421 gaagttttgt taagggtgtc ccggcagtac cagaaaatgg aatgtttgaa attccaaaac
      481 agctcgctgc ccaactgcca atcgggtcaa tacatctaaa tacaacggtc acaaatattt
      541 cgcctggaat tgttcaaact actgaaggtt cgattagctg tagagcaatt attttggcgg
      601 caaatgcacg cagtgcagca acattaatcc caagcctgaa ggtcccacca agtaattctg
      661 tgactacttg gtaccactta gctgattgtg caccgcaaga tttaactgaa ggtgcaggca
      721 cgcttatcgt tgacggtaag cgctacagtt ctcatgcgat agacccaaat aggccggtgg
      781 tgaatactgt ggcgatttct aacgcagcgc caaattattc gggtgggaat cgggtattag
      841 tttcagcgag tacaaccggt gttcatcaca ctatggctgg tgagtcagca gttcgtaaac
      901 acttggctac gctttataaa gtttcaacca ctacttggca acatgtggga acctacccaa
      961 tacctgatgc cttgccagcc atgttgccgc catttaattt agcgaaatca ccaagacttg
     1021 gtgatggtgt ttatgttgca ggggactatc gcgatgtgtc gtcgataaac ggtgcgttca
     1081 gaagtggaag aattgccgcc gaagctgcaa ttgcagacag tttttaacta agttttccaa
     1141 cgggtaaaac agtttcaacc acttcactat ttattttttc tactcgatat gcgcgagccg
     1201 actgatcggc tttatcaatt acaacttcaa ctggatctat cccgtggtaa acaattccag
     1261 catgatcgtc agtagcaaaa gacagcggca atgtttgggc ttgaactag
//
LOCUS       DBNHVN010000130         1389 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_171708, whole genome shotgun sequence.
ACCESSION   DBNHVN010000130 DBNHVN010000000
VERSION     DBNHVN010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1389)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_171708"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="AC16Y1_02385"
     CDS             complement(<1..253)
                     /locus_tag="AC16Y1_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR5831347.1"
                     /translation="MSKPRVLSGIQPTHDSFHIGNHLGALKNWVAMQDTHDCVYFIAD
                     LHAITVEHDPKLLKERTLVSYAQLLALGIDPEKSTLLVQS"
     gene            complement(266..>1389)
                     /locus_tag="AC16Y1_02390"
     CDS             complement(266..>1389)
                     /locus_tag="AC16Y1_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020392994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR5831348.1"
                     /translation="SRFLGAVQVGVTLAGFLSAGYGASRLVPIFSPFLVDLGLAEGIS
                     ETIAFVLGTLLIAYLSLVVGELTPKRLALQHAERYALFLSGSVDVMAKVTAPFIWLLS
                     KSSDVIVRLLGGDPSAGKEQISEEELRGMVAAHEELTHEERELIADVFSAGDREIKEI
                     MIPRTEVAFLDSDLPVFKAVKIIADKPHSRYPVADESQDDVIGFVHVRDLLDPELQGR
                     SIRIGDLAREVLRLPGSKQLIPTLTQMRAASAHMAIVVDEYGGTAGIITLEDLVEELI
                     GDIRDEYDTDESEGVIKPDTDLVVDGLLNLDDFADETSFELPDGPYETAAGFIASQLG
                     RLPMVNDEVTVPGGKLTVVEMDGRRVSRIHVVRQPQNRA"
BASE COUNT          386 a          337 c          301 g          365 t
ORIGIN      
        1 ggctttgcac tagcaatgtc gatttttctg gatcgatacc gagggctaac aactgcgcgt
       61 acgaaactaa agttcgttct tttagtaact ttgggtcgtg ctctacggta attgcgtgta
      121 agtcagcaat gaaataaacg cagtcgtgag tatcttgcat ggcaacccag ttctttagcg
      181 caccgagatg atttccaatg tggaaactgt catgcgttgg ttggatacca gatagaactc
      241 tcggtttact cacttgacta ttgtgtcatg ccctattttg aggttgtcgc acgacatgta
      301 ttcgagaaac tcggcgtcca tccatctcaa ctacggttaa ctttccgccg ggcacggtta
      361 cttcatcgtt aaccatcggc agtcgtccta attgcgatgc gataaatcct gcagcagttt
      421 catagggacc gtctggcaac tcaaatgaag tttcatcagc gaagtcatca agatttaata
      481 ggccatcaac aactaaatcg gtatctggct tgattacacc ttcggattca tctgtgtcgt
      541 attcgtcacg aatgtcacca atgagttctt caactaaatc ttcaagggtg ataatgcctg
      601 cggtgccacc atattcatcc accacaattg ccatgtgtgc gcttgccgcg cgcatctgag
      661 tcagagtggg aattaattgc tttgaaccgg gtaggcgcaa aacttcacga gctaaatcgc
      721 caattcgaat tgagcgaccc tgtaattcgg ggtcaagcag atcgcggacg tgcacaaaac
      781 caatcacatc atcttgagat tcatcggcga ctggatagcg tgagtgaggt ttatccgcga
      841 tgattttcac tgccttaaat actggtaaat cagaatcaag gaacgcgact tccgttctcg
      901 gaatcataat ttctttaatc tcacgatcgc cagcactaaa aacatcagca ataagttcac
      961 gctcttcatg ggttagttct tcatgtgctg ccaccatgcc gcgtaactct tcttcagata
     1021 tttgctcttt tccggcactc ggatctccgc caagtaaacg aactattacg tcactggatt
     1081 ttgaaagtag ccaaataaat ggcgctgtca ctttggccat cacatcgacg gatcccgata
     1141 aaaatagggc atagcgctct gcgtgctgca atgccaaacg cttcggggta agttcgccaa
     1201 ctactaatga aagataggca ataagtaacg taccaagtac gaacgcgata gtttcagaaa
     1261 ttccttcagc caaaccaagg tcaactaaaa acggtgaaaa aattggaacc aagcgtgaag
     1321 ctccataccc agctgacaag aatcccgcta aagtaacacc aacttgaacg gcgcccaaga
     1381 agcgacttg
//
LOCUS       DBNHVN010000131         1126 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_25772, whole genome shotgun sequence.
ACCESSION   DBNHVN010000131 DBNHVN010000000
VERSION     DBNHVN010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1126)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_25772"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..745
                     /locus_tag="AC16Y1_02395"
     CDS             146..745
                     /locus_tag="AC16Y1_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation efflux transmembrane domain-containing
                     protein"
                     /protein_id="HIR5831349.1"
                     /translation="MAGSVFLYLNLKRTVLLVAALNFIYFGVEFTFGRIYNSLALISD
                     SIDFLEDASVNLLVVLALGWSVAKRKYVSYLLAALLLVPGIAFLWNAVDKLLDPVAPV
                     GEGMTIVGLGALAVNVFCALLIARHKSEEHGLVLAAYYSARNDAIGNVLIIISGLITL
                     LTPSIWPDLLVGLFIFILNAGAAKSVIGASKADEKKSQS"
     gene            776..>1126
                     /locus_tag="AC16Y1_02400"
     CDS             776..>1126
                     /locus_tag="AC16Y1_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR5831350.1"
                     /translation="MTKEFKSLLLCIAGGIFFGTAGTAANLIETDSDSGTIGFSRILV
                     GAIALLIVAPLFGGKYTQIPSLLKKKEIYFMALGAGMYQPFFFGAVERTGVAWGTLVA
                     VGSIPVFAGTINWLL"
BASE COUNT          309 a          214 c          248 g          355 t
ORIGIN      
        1 aaattggaag tgcatgtgaa gtttcaagtc atggtcaaat gcatactggt tatgtgcaat
       61 ggcaaaaaaa tggtgacaac attcaattgc aatgcgtcat aacttaaatt gcttattaac
      121 tgagccagcc atataggatt gagaaatggc tggctcagtt tttttgtacc taaatctgaa
      181 aagaaccgtt ttattagtag ccgcacttaa cttcatttat ttcggagtcg aatttacttt
      241 tgggcgaatc tacaattctc tagccctaat cagcgacagc attgattttc tagaagatgc
      301 atctgtaaat ctgttagttg ttctagctct cggttggtca gttgccaaac gtaaatacgt
      361 tagctattta ttggctgcac ttttacttgt tccaggaatc gcatttctgt ggaatgcagt
      421 agataaacta cttgatccag tagcccccgt cggtgaagga atgacaattg ttggcttagg
      481 agcccttgca gtaaatgtat tctgtgcgct tttgatcgca cgacataaat ctgaagaaca
      541 tggattagtt ctagcggctt attactcagc gcgtaatgac gcgattggaa atgttctaat
      601 tattatttcc ggattaatca cactactaac cccaagtatc tggccagact tgttagtagg
      661 gctttttatt ttcatactta atgccggcgc agcaaaatca gttattggcg caagtaaggc
      721 tgatgaaaag aagtcgcagt cctaacaccg ttccacatca cgctaggtta gttcagtgac
      781 caaagaattc aagagccttt tactttgcat tgcaggcggt atattttttg gtaccgcagg
      841 tacagcagct aatttgattg aaacagattc agattcggga acaattggat tctccagaat
      901 tcttgtaggt gctattgcgc ttttaatcgt cgcgccatta tttggcggta agtacaccca
      961 gataccgagt ttgttaaaga aaaaagaaat ttactttatg gcccttggtg ccggaatgta
     1021 tcagccgttt ttctttgggg ctgtagaacg aaccggtgtg gcttggggaa ccctcgtcgc
     1081 agtaggaagc attccagttt ttgctggaac aattaattgg ttacta
//
LOCUS       DBNHVN010000132         2416 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_248483, whole genome shotgun sequence.
ACCESSION   DBNHVN010000132 DBNHVN010000000
VERSION     DBNHVN010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_248483"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..1111)
                     /locus_tag="AC16Y1_02405"
     CDS             complement(65..1111)
                     /locus_tag="AC16Y1_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019066332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HIR5831351.1"
                     /translation="MDQRLAIGIFGDKVVTDLLQVTHEPKDLDASGWWAVAQTFEGEF
                     TAFKFGTVTPIDEFDFDNRVFRPVSPLGWHSSMQQYEYEDAVKSIQEDISRGWVYQVN
                     LCYVLTNEIETPFDPIAFYFELRKHNPAPYLSAIFVPAHQSGLAEDTAITSASPELFI
                     KRKDNLIKSSPIKGTAKTEGQLLEKDRAENVMIVDLIRNDLSRVALDGTVSVPDLLRV
                     EPHPGLVHLVSDVSGTVDPKTSWREILQAMSPPGSVSGAPKSSALEVISRLEPVARNI
                     YCGAFGFVDSDNDEAVLAVGIRTFWQSSKAGKTYLNFGTGAGITWGSTPELEWYETQL
                     KAKNLLKVAGAEIE"
     gene            1188..1259
                     /locus_tag="AC16Y1_02410"
     tRNA            1188..1259
                     /locus_tag="AC16Y1_02410"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1220..1222,aa:Gly,seq:gcc)
     gene            1294..1364
                     /locus_tag="AC16Y1_02415"
     tRNA            1294..1364
                     /locus_tag="AC16Y1_02415"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1326..1328,aa:Cys,seq:gca)
     gene            1381..1454
                     /locus_tag="AC16Y1_02420"
     tRNA            1381..1454
                     /locus_tag="AC16Y1_02420"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1415..1417,aa:Val,seq:gac)
     gene            complement(1515..>2416)
                     /gene="zapE"
                     /locus_tag="AC16Y1_02425"
     CDS             complement(1515..>2416)
                     /gene="zapE"
                     /locus_tag="AC16Y1_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015748721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HIR5831352.1"
                     /translation="EAEHIVEFFTPPPRFAKVRFSNYQPNPEHPSQNVALEAMQNFAN
                     PDIPKKSKWPRKSKSDPGKGIYLDGGFGVGKTHLLAALYHEFSGKVAYASFVEYTNLV
                     GALGFEKTVEALSNFDLICIDEFELDDPGDTVLMSTLLNRLVDLGTNLAATSNTLPDR
                     LGEDRFAADDFIREIQGLAAHFEVLRIDGPDFRHRENEKSALPGTDAQLLNGGDDVSF
                     DFMSALDSHLQKVHPSAYAKLFTGLTQVNVANLFQLSNQANALRWVVFIDRMYDQEIP
                     FAYSGELIDELFSQEMKKGGYRK"
BASE COUNT          659 a          580 c          492 g          685 t
ORIGIN      
        1 tagcaacgtc actactaaaa atttcaccat ttacataact gaaagtttcg gacacaaatc
       61 aactctattc aatttctgcg ccagcaactt tcagtaagtt ctttgccttt aattgagttt
      121 cataccattc gagttcaggt gtactgcccc aagttatccc ggctccggtt ccaaaattta
      181 gatacgtctt tcctgcttta gaggattgcc agaaggttcg tattccaaca gccaacaccg
      241 cttcatcgtt atcactatct acaaatccaa atgctccgca ataaatgttt cgagcaaccg
      301 gctctaacct tgaaataact tctagtgcac tactttttgg agctccacta acagatccag
      361 gtggactcat tgcttgcaag atttcgcgcc atgatgtttt gggatcaaca gttccgctga
      421 catcactaac taaatgaaca aggccaggat gtggctcgac tcttagcagg tctgggacgc
      481 taacggttcc gtcaagtgcg actcgagaaa gatcattacg aatcagatcg actatcatca
      541 cattttcagc gcgatctttc tccagcaatt gcccctctgt cttagccgta cctttaatcg
      601 gacttgattt aatcaaattg tctttccttt taataaataa ttcaggcgaa gcgctagtga
      661 ttgccgtgtc ttcagctaaa ccagattgat gtgcaggcac aaatattgca cttaaatacg
      721 gcgctggatt atgttttctt aattcaaaat agaacgcgat cggatcaaat ggagtttcta
      781 tttcgttagt caagacataa caaagattta cctggtatac ccagcctcga ctaatatctt
      841 cttgaattga cttaaccgca tcttcgtact cgtattgttg cattgatgaa tgccagccaa
      901 gtggacttac tggtcggaaa acacgattat caaaatcaaa ttcatctatg ggagttacag
      961 tgccaaattt aaaagccgtg aattcccctt caaaagtttg agcaaccgcc caccaaccgc
     1021 ttgcatctag atctttcggt tcatgcgtta cttgcagcaa atcagtgaca accttgtcgc
     1081 caaagattcc tattgctaag cgctgatcca tgtaaacacc ttattttgat ccgatagcgg
     1141 tttttgggat aaggtaacgc agtcgggttt attaataaat ccaacatgcg gacgtggctc
     1201 agtggtagag catcaccttg ccaaggtgag ggtcgcgggt tcaaatcccg tcgtccgctc
     1261 gaaaagaact cgcataccgt gagttgtttc agcggtggag tggccgagag gcgaggcagt
     1321 ggcctgcaaa gccgcgtaca cgggttcaaa tcccgtctcc acctcaaata cgaagacaac
     1381 ggtcgattgg cgcagttggt tagcgcgctt ccttgacatg gaagaggtca caggttcgag
     1441 tcctgtatcg accacgctta ttgctttttt acctcgcggc ttagcgcact caatcttgaa
     1501 agtgcccgaa aatattattt tcggtagccg ccctttttca tttcctgcga aaaaagttcg
     1561 tcaattaact cgccactata cgcaaaaggt atttcctgat cgtacatacg gtcgataaaa
     1621 actacccagc gcaacgcatt ggcttgatta ctaagttgga ataagttagc tacatttact
     1681 tgagtcaagc cagtgaacag ctttgcataa gcacttggat ggactttttg tagatgacta
     1741 tctagtgctg acatgaaatc aaacgaaaca tcgtcgccgc catttagtaa ctgcgcgtct
     1801 gttcctggca gcgcactttt ctcattttct cgatgacgga aatcaggacc atcaattcgc
     1861 aacacttcaa aatgagcggc aagtccttgg atctctcgga tgaaatcatc tgcggcaaat
     1921 cgatcttcgc caagtcgatc gggcaacgta ttactagtag ccgcaagatt cgttcccagg
     1981 tccaccaacc ggttgagtaa tgtgctcatc aaaaccgtgt caccaggatc gtctaattca
     2041 aattcatcta tgcagatcaa atcaaaattg ctaagagcct caaccgtttt ttcaaaacca
     2101 agcgcaccca caaggtttgt gtattcgaca aaactcgcat aggcaacttt gcctgaaaat
     2161 tcgtgataca gcgcagcgag taagtgagtt ttccctacgc caaagccacc atctagataa
     2221 ataccttttc ctgggtcaga tttacttttg cgcggccact tactcttttt tgggatatca
     2281 gggttagcaa aattctgcat ggcttccaat gccacatttt gagacgggtg ctctggattt
     2341 ggctggtaat ttgaaaatct aaccttcgca aatcttggcg gtggtgtgaa aaattcaaca
     2401 atatgttcgg cctcta
//
LOCUS       DBNHVN010000133         1709 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_98626, whole genome shotgun sequence.
ACCESSION   DBNHVN010000133 DBNHVN010000000
VERSION     DBNHVN010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_98626"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="AC16Y1_02430"
     CDS             <1..511
                     /locus_tag="AC16Y1_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013122.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR5831353.1"
                     /translation="RLSADGQVKGTLSTLINPEIPTTGARHIHGISFEMVESAPTYKQ
                     VFDQFADLLDGAIFVAHHVKFDEGFISAESYRAGVKLNRMPGLCTYWLSRQVLQLPNY
                     KLATVTNHFGIEQELAHSAYDDALVVSRFLPELLASYGSVEQYVELTDQPRVGQSAEL
                     FPRKTEFFD"
     gene            complement(508..1143)
                     /locus_tag="AC16Y1_02435"
     CDS             complement(508..1143)
                     /locus_tag="AC16Y1_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015793890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5831354.1"
                     /translation="MKLDHVSYAVLNNELADTVQRLGADLGASFIDGGKHPRFGTRNF
                     ILPLNNGAYIEVVAPLDHPAAENVPFGQAVRRRAEAGGGWLSWVLAVEDLAPLEAKVG
                     RAAGPGHRVRPDGVELRWNQIGVIDLLDDPQLPFFIKWDSDSAHPSTAATNSISVTQI
                     SLSGSPTSVKDWLGDGYQQVTQNVNLSWVEDDETGINSVSFNTPSGTITID"
     gene            1387..>1709
                     /locus_tag="AC16Y1_02440"
     CDS             1387..>1709
                     /locus_tag="AC16Y1_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831355.1"
                     /translation="MELTELEKTRDRVCAEISSDSLPENLDQLINNIVEIPIRDGLLR
                     FLYDNESLRQKSANNLRALLETDCGFARCPIATVLAGCCWLDSNLIETRKALNLALEI
                     DSTYSL"
BASE COUNT          463 a          387 c          390 g          469 t
ORIGIN      
        1 caggcttagt gccgatggtc aggttaaagg aacactctcg actttgatca atccagagat
       61 tccaacaacc ggagctcgtc acatacacgg aatctcattt gagatggtgg aaagtgcgcc
      121 tacgtacaaa caagtttttg atcagttcgc cgaccttttg gatggcgcaa tatttgttgc
      181 gcatcacgtg aaatttgatg aaggctttat ttctgccgag tcttatcgtg ccggagttaa
      241 attgaaccga atgccaggac tttgtactta ttggttaagt cggcaggttt tgcaattacc
      301 taactacaaa ttagccaccg tgaccaatca ctttgggatc gagcaagaat tagcacactc
      361 tgcctatgac gacgcattag tggtatctag attcttgccc gaattacttg caagttatgg
      421 cagcgttgaa cagtatgttg aattaactga tcaaccacgc gttggacaaa gtgctgaatt
      481 atttcccaga aaaactgagt tttttgacta gtcgatagtt atggtgccac ttggggtgtt
      541 gaacgaaaca ctatttatcc cagtttcatc atcttcaacc caagacaaat taacgttttg
      601 cgtaacttgt tggtagccat caccaagcca gtctttaact gacgttggtg aacccgaaag
      661 agaaatctgg gtaactgaaa ttgagtttgt tgccgcagtt gagggatgcg cggaatctga
      721 atcccacttg ataaagaaag gtaactgtgg gtcatcaagt aagtcgatga ctccgatttg
      781 attccagcga agttctactc catctgggcg aacacgatga cctggtccgg cagcgcgacc
      841 aacttttgct tcaagtgggg ctagatcttc aacggccaaa acccaagaca accagcctcc
      901 gccagcttcg gcgcgacgac gaaccgcttg accaaatgga acattctcag ccgctggatg
      961 atctagtggc gctactactt caatgtatgc gccgttattc agcggcagaa tgaagtttct
     1021 tgttccaaag cgtgggtgtt ttccaccgtc gataaatgat gcacctaaat cagcaccaag
     1081 acgttgcaca gtatctgcaa gctcattatt aagaacggcg tacgagacgt ggtctagctt
     1141 cataaagaaa ctttggcaca ggggtggaac agcagtcaca ttggggtcgg attttcacaa
     1201 aggtgcattg cggcgtgtct aaggctgcat ttcccttgcg ttgtctaaat taatccctag
     1261 cacccagtgt gacggcggag gggaagccac cgaagcgctg ggtgtctttt ttgcccacca
     1321 tcctgtttgc tccacagtta ttgaatgcca tagaaatgct gctaattcgc aatgcattat
     1381 ccgctcatgg aattaacaga acttgaaaaa actcgcgatc gcgtttgtgc ggaaatatca
     1441 tccgactctt tacccgaaaa tcttgatcag ttgattaaca acatagtaga aataccaatt
     1501 cgtgatggac ttcttcgctt cctatatgac aatgaatcct tgcgacaaaa gagtgcgaat
     1561 aatcttcgag ctttacttga aacagattgc ggttttgcca ggtgcccaat tgccacagtt
     1621 cttgctggct gctgctggct tgattcaaac ttaattgaaa cgcgaaaggc tctgaattta
     1681 gcccttgaaa tagattcaac atattctct
//
LOCUS       DBNHVN010000134         1146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_692998, whole genome shotgun sequence.
ACCESSION   DBNHVN010000134 DBNHVN010000000
VERSION     DBNHVN010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_692998"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..668)
                     /gene="pgsA"
                     /locus_tag="AC16Y1_02445"
     CDS             complement(72..668)
                     /gene="pgsA"
                     /locus_tag="AC16Y1_02445"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR5831356.1"
                     /translation="MKNPVPDHVGDPVSGPSSVNLPNILTAFRLVLVPIFIYYLTIFS
                     DSIFNQWIAALFYFVAAMTDVLDGYLARKNNQITNWGKIADPIADKALTGSAFIGLSL
                     LGVIAWWITVIILVRELGVTLLRFYVIKRGVIPASRGGKAKTLSQNIAIVAFLIPLPT
                     WLEFFPVAVLYFAVILTLATGFDYVRKAQVLMASKKGN"
     gene            complement(732..>1146)
                     /locus_tag="AC16Y1_02450"
     CDS             complement(732..>1146)
                     /locus_tag="AC16Y1_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR5831357.1"
                     /translation="QGDGLFLPMGMSKPIRIQCSWVTEAEIRAVVAHAKTQMEPQYRE
                     EITAPVAAKREVDSDIGNDLDDLLAAVELVVSTQFGSTSMLQRKLRIGFAKAGRLMDL
                     METRGIVGPSEGSKAREVLITAEDLPGTIAMLRGE"
BASE COUNT          352 a          266 c          224 g          304 t
ORIGIN      
        1 gctgtagcaa ctgtgtggcc atcaagggca atgacaacag ttaaagcaag tgaatctaaa
       61 tctgtttgca tttaattgcc tttcttgctg gccatcaata cttgagcttt gcgcacgtaa
      121 tcaaagccag ttgctagcgt caaaattacc gcaaaataca aaactgcgac tgggaaaaat
      181 tcaagccatg ttggcagcgg tatcaaaaat gcaactatcg caatgttttg actcagcgtt
      241 tttgccttcc cacctctact tgctgggatg acgccacgtt tgattacata aaaacgaagt
      301 aaagtaacac caagttcacg aaccaaaata atcacagtta tccaccaagc gattacgcca
      361 agtaaagaca agccaataaa agctgacccg gttagtgctt tatctgcaat cggatcagcg
      421 atcttgcccc agttagttat ttgattattt tttctagcca gatatccatc gagcacatcg
      481 gtcattgcgg caacaaaata aaacaaagcc gcgatccatt ggttaaaaat agaatcgcta
      541 aaaatcgtta agtagtaaat aaaaattggc actaagacca ggcgaaatgc cgtcaaaata
      601 tttggcaggt ttactgacga aggtccagaa accggatcac caacgtgatc gggtaccgga
      661 ttcttcattg catccctctg ctagttgcta cgttcgtgaa gtaaatcttg tcgtaagtta
      721 ataagaaaag actattcgcc gcgcaacatt gcgattgtgc ctggtaagtc ttcagcagta
      781 attaaaactt cacgagcttt agaaccttca gacggtccaa ctattccacg agtttccatt
      841 agatccatca aacgaccagc tttagcaaat ccgatgcgca gtttgcgctg caacattgaa
      901 gttgaaccaa attgagtaga aactaccaac tcaacggcag ccagtaagtc atctaagtca
      961 ttaccaatgt cagaatcaac ttcacgtttt gccgcaactg gcgcagtaat ttcttcgcga
     1021 tactgcggtt ccatctgtgt ctttgcgtga gctacaactg ctctaatttc agcctcggta
     1081 acccatgagc attgaattcg aattggtttg ctcataccca ttggtaagaa caatccatcg
     1141 ccttgg
//
LOCUS       DBNHVN010000135         5712 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1362952, whole genome shotgun sequence.
ACCESSION   DBNHVN010000135 DBNHVN010000000
VERSION     DBNHVN010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1362952"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1085
                     /locus_tag="AC16Y1_02455"
     CDS             <1..1085
                     /locus_tag="AC16Y1_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831358.1"
                     /translation="LNARITELEGAVGRPTFIDMDDDALTQIAAEDAAVIIRAARTRA
                     TKLVEQASETLQKAESDREQIRASAELDARSIREAANGDARKLRAEATSTLETARIQA
                     EQLLAASQDDADEQLKVTQATIAKLLEDATRQADDVIKSANNLRSRTEKEVAVLRAEA
                     EKQIDEEVLASQNEINRNLDSATTEAKRLVSEATQAAQAMVEKTSGDTRATLSETQAR
                     IDNMLREAQQKADSLVAEGQAKLKESEQAAKRQAAEILENARNKSNEIKRTALMYSDH
                     IKESIDNEKRIIVELNNQISKVRTGWLESQTTISELLSEINTTFVGVENSVAEATQKI
                     SNKRTDVTSTLTEMTARINEESAGKE"
     gene            1143..2051
                     /locus_tag="AC16Y1_02460"
     CDS             1143..2051
                     /locus_tag="AC16Y1_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019049685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5831359.1"
                     /translation="MRVDVTNLDKHFGKTHAVRDLSFSIEPGRVTGFLGPNGSGKSTT
                     MRLMLDLDRGKGKTLYGGKRLTQLNPVTKAVGAHLDAKFFHPKRTAFAHLNMLGAEAH
                     VSKQRINEVLELVGLTSVAKKRPGGFSLGMGQRLGLAGAILAKPSVLFLDEPANGLDP
                     QSIQWLRDFLKNYAAEGNSVFVSSHLLSEMQLLADDVVVIAAGKLIAAESMQQFLTRA
                     QGTNVVVRSNELAKLRDFIGSEGIESRFEGSNTLLVPGVTTDRIGEIAFKNNIQISEL
                     TMRSASLEQVFLELTDQGQEFRVGGK"
     gene            2052..2795
                     /locus_tag="AC16Y1_02465"
     CDS             2052..2795
                     /locus_tag="AC16Y1_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831360.1"
                     /translation="MFRALVFEWRRLWSVRATWIMLFVFTAVNSFFGIYPLFSGRVPG
                     MQSWLGLYNTPANFLSLVLLSVVAAQVFGHEYRYGTIRLTLTEFPKREIVMFAKTLIL
                     GFYVSFALILNWGVLGALAQLAPEGTITSDSPGFTFGPYNPADLWKVYLYVLGYVFIA
                     MSITAITRNLAIGIVFPLLLTVIEGLLGLLNQLAKQRFDWLVERLPLENATDWLGNGG
                     MYVSPGLTYAAWVVAFYLAASALFFKRDA"
     gene            2792..3556
                     /locus_tag="AC16Y1_02470"
     CDS             2792..3556
                     /locus_tag="AC16Y1_02470"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR5831361.1"
                     /translation="MTQLNKSELQRTVLIGLLAGVLAGLFGVGGGFLMVPLFISWLAV
                     DQKQAHATSLGAVFPIAISGAFGYATTDHVDWQSGGYLLLGSFFGAMYGAKLLHTISL
                     TKLQFTFGTLLILSALRLLWSAQPTQLVEGVVGHILLIIVGLFAGTMSGLLGIGGGII
                     MVPAMIIAAGLTAVEARGTSLAVIVGSSISGAVSHYKRGNIIIPIALVSGIAGVPATF
                     VGIYLSQNLPERISVALFAARLLTMAAQQFKKALNK"
     gene            complement(3682..3768)
                     /locus_tag="AC16Y1_02475"
     tRNA            complement(3682..3768)
                     /locus_tag="AC16Y1_02475"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3732..3734),aa:Ser,seq:cga)
     gene            complement(3805..4236)
                     /gene="tadA"
                     /locus_tag="AC16Y1_02480"
     CDS             complement(3805..4236)
                     /gene="tadA"
                     /locus_tag="AC16Y1_02480"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIR5831362.1"
                     /translation="MQVALDLAKSAAEVGDVPVGAVVVDQQGSIIGRGFNTREVAHNP
                     LGHAEVNALNDAAKSLGSWRLDDCTLVVTLEPCTMCAGAIVQSRVGRVVFGAFDDKAG
                     AIGSLWDVVRDPRLPHRPEVVSGVLASEASELLSEFFKNKR"
     regulatory      4332..4440
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00634"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class IV; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00634"
     gene            4507..5385
                     /locus_tag="AC16Y1_02485"
     CDS             4507..5385
                     /locus_tag="AC16Y1_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019126645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5831363.1"
                     /translation="MTNANFRRIRNSVAALALTALVAACSPQSETSTAPSADATAANS
                     CSADSLQTLTTGKLTIATGEPAYYPWVIDDNPESGEGFEAAVAYAVAEELGFAKEDVV
                     WERTTFDSAVLPGAKTFDFNLQQFSITEERKAAVDFSSPYYNTTQAIVSIKGSAIEGV
                     TAIADLKDAKLGAGVGTTSLDVIKNQIGVEPQVFNDNAAAVQALINGQIDGIVVDLPS
                     AFYVSAVQIESGDGIIVGQLPATGEGDNFGLLLAKDSPITSCVSQAVDALRADGTLDS
                     IAATWLGTDAGAPVLK"
     gene            5405..>5712
                     /locus_tag="AC16Y1_02490"
     CDS             5405..>5712
                     /locus_tag="AC16Y1_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831364.1"
                     /translation="MNNDSPARNNSGRALSWTPSVREQERQITRSKIARKRITIAAVS
                     TFLVLGALFGGLVSSPGWPVVKKTFFDWEYGQTVISTVIRGLITNITLTVVASIAIGI
                     "
BASE COUNT         1615 a         1232 c         1283 g         1582 t
ORIGIN      
        1 aactaaacgc tcgcataacc gaacttgaag gcgccgtcgg ccgtccaact ttcatcgaca
       61 tggatgatga tgcgttaact caaattgccg ctgaggatgc agcagtaatt attcgcgctg
      121 ctcgtactcg cgctaccaaa cttgttgaac aagcatcaga aactctgcaa aaagcagaat
      181 cagaccgtga acaaattcgt gcaagtgccg aattagatgc ccgcagtatt cgcgaagctg
      241 caaatggcga tgctcgaaaa cttcgcgctg aagctacctc aactttagaa actgctcgta
      301 ttcaggcgga acaattactt gcggcttcac aagatgatgc cgacgaacaa ttaaaagtta
      361 ctcaagcgac tattgccaaa cttcttgaag atgcaacgcg tcaagctgac gatgtaataa
      421 agagtgctaa taatttacgt tcacgcactg aaaaagaagt tgccgtactt cgtgccgaag
      481 ctgaaaagca aattgacgaa gaagttctag catcacaaaa tgaaatcaac cgaaatctag
      541 atagtgctac gactgaagca aagcgccttg ttagcgaagc aacccaagca gcacaagcaa
      601 tggtcgaaaa aaccagtggt gatacacggg caacactttc tgaaactcaa gctcggatcg
      661 acaacatgct tcgcgaagcg cagcaaaaag cagactctct agttgctgag ggtcaagcaa
      721 aacttaaaga atctgaacaa gcagcaaagc gtcaagcggc tgaaattttg gaaaatgctc
      781 gaaacaaatc aaatgaaatc aagcggaccg cattgatgta tagcgatcac atcaaagaat
      841 caattgataa tgaaaagcgg attatcgttg agctaaataa ccaaatttca aaagtacgta
      901 ccggatggct tgaatctcaa acaactatct cggagttact aagtgaaata aacacaactt
      961 ttgttggcgt ggaaaattct gtagctgaag ctactcaaaa aattagcaac aaacgtaccg
     1021 atgtaacttc gacattaact gaaatgactg cgcggattaa cgaagaatct gcaggcaaag
     1081 aatagattta ctgcttcaaa agccgtatgt ctatcaggtc tggtaagcaa tagagttaag
     1141 tcatgcgcgt tgacgtaacg aatctcgata aacactttgg caaaactcat gctgttcgcg
     1201 acttatcatt ttcaattgaa cctggtcgag taaccggatt tttgggtcca aatggttctg
     1261 gtaaatcaac caccatgcgt ttaatgctcg acctcgatcg gggtaaaggt aaaacacttt
     1321 atggtggaaa acgcctaacc caactaaacc ctgtgactaa agccgtgggt gcgcatttgg
     1381 atgcaaaatt tttccatcca aagcgcaccg cattcgcaca cttaaacatg ctgggcgcag
     1441 aagcgcatgt ttcgaaacaa cgcattaatg aagttttgga attagttgga ttaacttcag
     1501 ttgcaaaaaa gcgaccgggg ggattttcac ttggaatggg ccagcgactt ggtttggctg
     1561 gtgcaatttt ggctaaaccc tcggttttat ttctagatga accagcaaat ggtttagacc
     1621 cacaatctat tcagtggtta cgtgattttc taaaaaatta tgccgctgaa ggtaattcag
     1681 tatttgttag tagccactta cttagtgaga tgcagttatt agctgacgat gtagttgtta
     1741 ttgcagcggg caagttaatt gcagccgagt caatgcaaca atttttaacc agagcacaag
     1801 gtacaaatgt tgtggtgcgc agtaatgaat tggcgaaatt acgtgacttc atcggtagtg
     1861 aagggattga atcaaggttc gaaggttcaa atactctttt ggtgcccggc gtaaccaccg
     1921 atcgaatcgg agaaattgcc tttaaaaaca atattcaaat ttcagaatta actatgcgtt
     1981 cagccagcct ggagcaagtc ttccttgagt taactgatca aggacaagag tttcgagtag
     2041 gtggcaagta gatgtttcgc gcattagttt ttgagtggcg cagattatgg tctgttcgag
     2101 ctacttggat catgcttttt gttttcacgg cagtgaattc gttttttggt atttacccgt
     2161 tattttctgg ccgcgtacca ggaatgcaga gttggctagg tctatacaac acaccagcaa
     2221 atttcttaag tttagtttta ctttcggtag ttgccgctca agtattcgga catgaatatc
     2281 gctacggaac aattcgactg accctgactg aatttccaaa gcgtgaaatt gttatgttcg
     2341 ctaaaacttt aattttaggt ttttatgtat cttttgcgtt gattttaaat tggggtgtac
     2401 ttggcgcact agcgcaacta gctccagaag gaacaattac ttctgactca cctggattta
     2461 cttttggtcc ttacaaccct gcggacttat ggaaagttta tttatacgtt cttggctacg
     2521 tgtttattgc gatgagtatt actgcgataa cgcgtaactt ggcaatcgga atcgtttttc
     2581 cattgctatt aacagttatc gaaggtttgc ttggtttatt aaatcagctt gcaaagcaac
     2641 gattcgattg gttagttgaa cgtttacctc ttgagaatgc aactgactgg ttaggtaatg
     2701 gtggaatgta tgtttcgcca ggacttactt acgcagcttg ggtagttgct ttctacctag
     2761 cagcaagtgc gctcttcttt aaacgagatg catgacgcaa cttaataaaa gtgaactgca
     2821 gcgaacagta ttaataggtt tacttgctgg agttcttgct ggactttttg gtgttggcgg
     2881 tggcttcctt atggttccac tttttatctc atggttagca gttgatcaaa aacaagctca
     2941 cgccacttcc ctaggtgcgg tatttccgat cgcgatatcc ggagcatttg gctatgccac
     3001 aactgatcat gttgattggc aatcaggtgg ctacctatta ctcggttcat tttttggcgc
     3061 aatgtacggt gccaaacttt tacacactat ttcgcttacc aaattgcaat tcacttttgg
     3121 aacgctatta atactgtctg ccctgaggtt attgtggtct gcgcaaccaa ctcagttagt
     3181 tgagggagta gtcggccaca ttcttttaat tatcgttgga ttatttgccg gaactatgtc
     3241 gggattactt ggcattggtg gaggcatcat tatggtgcca gcgatgatca tcgccgctgg
     3301 gttaacagca gttgaggcta gaggaaccag cttggcagta atagttggtt cttctatttc
     3361 aggtgcagtt tcacattaca aacgtggaaa tataattatt ccgattgctc tagtaagtgg
     3421 gattgctggg gttccagcaa cttttgttgg tatttattta agccagaatt taccggaacg
     3481 tatctcagta gcactatttg ccgcaagact tttaacaatg gccgcacaac aattcaagaa
     3541 agcacttaat aaatagcaaa ctagcaagcg cggtatcggt gggaaccctt gttcgtgcta
     3601 ttcgcacgaa ctgcgtccca aatcccacaa actgattatt cgcgcatgca aacaatctcc
     3661 gaaatgagat tgttgcaagc gcggtatcgg tgggatttga acccacggtg cgcttgcacg
     3721 cacacaactt ttcgagagtt gcaccttcgg ccgctcggac acgataccgc caactaggtt
     3781 acttgataat taaccgttta aaaattaacg cttatttttg aaaaattcac tcagtaattc
     3841 cgatgcttca cttgctaaaa ctcctgaaac tacttctggt ctatgcggta aacgtggatc
     3901 tcgaactaca tcccaaagtg atccaatcgc accggcttta tcgtcaaagg cgccaaaaac
     3961 aacgcgtccg actcttgatt gaactattgc gccagcacac atagtgcaag gttctaaggt
     4021 aacaacaagt gtgcagtcat ctagccgcca tgaacctaac gattttgcgg catcgtttag
     4081 cgcatttact tcagcatggc cgagtggatt atgcgctacc tcgcgtgtat taaatccgcg
     4141 accaataatg ctgccctgtt gatcaaccac aacagctccg accggaacat ctccgacttc
     4201 ggctgcagat ttcgctaaat caagcgcaac ctgcattgcg ggctcaaatt ttgcggtatc
     4261 cacttgccaa ttctggcatg caaaaaaggt cgagttggct tggtcagtca caacttgcta
     4321 ggctgtcaat cggtccagag agataacgtt aagtcccggc tagttatccg acaacccttg
     4381 caaccgcgat ggggtgcccc cgaggaatag accaggccaa gtaattggca agcgcgggtc
     4441 ccaacgggcc caacatgggt attcatccgt ttactcatgc cacttgaaag taaataggag
     4501 atcagcgtga ctaacgctaa ctttcgtcgc atccgcaatt cagttgctgc actagcacta
     4561 actgcacttg ttgctgcttg ttcaccgcag tctgaaactt caactgcacc gtcagcagat
     4621 gcaactgctg ctaactcatg ttctgcagat agcttgcaga ctcttaccac cggcaaacta
     4681 actattgcta ctggtgaacc tgcttactac ccatgggtaa ttgatgacaa tcctgaatca
     4741 ggagaaggtt ttgaagctgc tgttgcttat gcagtagccg aagaacttgg ctttgctaag
     4801 gaagatgttg tttgggaacg caccacattt gatagcgcag tgctaccagg tgctaagaca
     4861 tttgacttca acttgcaaca gttctcaatc actgaagaac gcaaagctgc tgttgatttc
     4921 tcaagcccgt actacaacac aactcaagca attgtttcga tcaagggaag tgcaattgaa
     4981 ggcgtaactg ctattgccga cctaaaggat gcaaagcttg gcgctggagt tggtacaaca
     5041 tcactagatg tgattaagaa tcagattggt gttgaaccac aagtattcaa cgacaatgca
     5101 gcagctgttc aagcactgat taatggacag attgatggaa ttgtcgttga ccttccatct
     5161 gctttctatg tgtctgcagt tcagattgaa agtggagacg gcatcatcgt tggtcaactt
     5221 ccagcaactg gtgaaggcga caacttcggt ctgttacttg caaaagatag cccaattact
     5281 agctgtgttt ctcaggcagt cgatgcacta cgtgcggatg gcacgttaga ttcaattgct
     5341 gctacttggc taggtacaga tgctggtgca cctgtactta agtaagtgct aacttactaa
     5401 caccatgaac aatgacagcc cggcccgaaa taattcgggt cgggctttgt catggacacc
     5461 aagtgttcgt gaacaagagc gacaaataac cagaagcaaa attgcgcgca aacgaataac
     5521 tattgctgcg gtttctacat ttttagtttt aggtgcgctt tttggtggat tagttagttc
     5581 accaggctgg ccagttgtta agaagacttt ttttgattgg gaatatggtc aaacagtaat
     5641 aagcaccgtt attcgaggct taatcacaaa tatcactctc acagtggttg cttcaatcgc
     5701 aattggcatt at
//
LOCUS       DBNHVN010000136         6459 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_173060, whole genome shotgun sequence.
ACCESSION   DBNHVN010000136 DBNHVN010000000
VERSION     DBNHVN010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6459)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6459)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6459
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_173060"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /gene="hutU"
                     /locus_tag="AC16Y1_02495"
     CDS             complement(<1..1054)
                     /gene="hutU"
                     /locus_tag="AC16Y1_02495"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HIR5831365.1"
                     /translation="MVFRLSGPRPVKAATGTKLSAKGWQQEAAIRMLMNNLDPNVAEH
                     PDELVVYGGTGKAARNWASFDAIVRTLTNLENDETMLVQSGKPVGVFQTHEWAPRVIL
                     ANSNLVGDWANWPEFRRLEQLGLTMYGQMTAGSWIYIGTQGILQGTYETFAAVAAKRF
                     GGTLAGTLTVTGGCGGMGGAQPLAVTMNEGVCLIIDIDETRLRRRVETRYLDEVTTDL
                     KDAVDRCLKAKNERRGLSVGLVGNAAEVLPKLLELDVPVDIVTDQTSAHDPLYYIPTG
                     IEFDQAREYADKKPDEFTDRSREAMAKHVEAMVGFMDKGAEVFDYGNSIRDEARKGGY
                     DRAFDFPGFIPAYIRPL"
     gene            1228..2142
                     /locus_tag="AC16Y1_02500"
     CDS             1228..2142
                     /locus_tag="AC16Y1_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017793497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR5831366.1"
                     /translation="MTNLPSDPLWPRAGHWLSTEAQSNPDLIYLGVPAHLTSISPTNA
                     QTTPDAVRDALLRYSTYSWSKNLDVTRCNFFDAGNVADPDYAEGAVRVAQKLAQLPAT
                     ELLIAVGGDNSITHSVAKSFWKENIGKAGLITFDAHHDLRDGESNGSPVRQLIAAGLQ
                     GTHVVQIGISDFANSPAYAKRAKEYGITVITRDAMRRRPLSEIMQEAIEIAGASGGPI
                     HVDVDVDVCDRSVVPACPAAAPGGISADELRQLVHLACLNTSVKSIDFTEIDASIDSP
                     DQRTVRLAALCILEAAAGVVIRTGKLED"
     gene            2172..3602
                     /locus_tag="AC16Y1_02505"
     CDS             2172..3602
                     /locus_tag="AC16Y1_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017956307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5831367.1"
                     /translation="MATLKNFIGGKLVDPIKGQMMDMENPATGQIYGHAPKSSPEDVD
                     LAFKAADASFDAWKRTSPADRSLALFRIADAMEKRASEIIAAEVQDTGKPVWWMRDME
                     MPMIVDHTRFHATLARNLPGLSTGEYLPGYDSTVRREPVGVCAQVAPWNYPMLMAVWK
                     FAPAIALGNTIVLKPSDTTPASAAILAEIFAEHLPAGVANIIYGDRDTGRAMVAHKRP
                     DMVSITGSVRAGMEIAANAAPDLKRVHLELGGKAPVIVFADCDFDRTVDEVATANVMN
                     AGQDCAAGCRVIVEESIADEFTRALAKRISEFNFGAPEEDKNYYGSLNSQAQLDRVQG
                     FIDRLPSHAQILTGGKASRLNGGYYFEPTLVAGLKQDDEMIQNEVFASVQTIQTFKDE
                     ADAIRMANDVAYGLAASIWSTNHQKIIRVSQDIDFGQVWVNCHLVQVGEMPNGGFKHS
                     GHGNDLSNEAIEGYTRVKHVMSYTGR"
     gene            3708..4706
                     /gene="argF"
                     /locus_tag="AC16Y1_02510"
     CDS             3708..4706
                     /gene="argF"
                     /locus_tag="AC16Y1_02510"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003863360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR5831368.1"
                     /translation="MDLRKRDFLKEIDFTAAEFQYLIDLAIELKREKKARKEKQRMVG
                     RSVALIFEKPSTRTRCAFEVAAFDQGAFTTYLDGSSSQVGHKESVADTARVLSRYYDA
                     IEYRGNEQELVEELAEYSDVPVYNGLTDQWHPTQMLADFMTMIEHSGKRPEEIKFTYV
                     GDARSNMGNSLLVTSALLGSDIRIASPKKLWPSQQIQDLATNLAKTSGAKLLLTESMA
                     DALPGSDFVHTDVWVSMGESEEIWKERVELLGDYQVNYETLAITQNPNAKFMHCLPGF
                     HNEQTTVGRAIANKTGMHEGLEVSHDVFEGPQSVVFDQAENRLHTIKAIMVATIGD"
     gene            4728..5792
                     /locus_tag="AC16Y1_02515"
     CDS             4728..5792
                     /locus_tag="AC16Y1_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014220369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HIR5831369.1"
                     /translation="MSKLFSPIKVGGIEIKNRAWVSPMCQYSAVNGLIQDWHAVHYGA
                     FITGGAGLVMVEATGVQEIGRITPGCVGIWTDQQAQVLAEVPRFAHRYGVKAGIQLAH
                     AGRKGSTAAPWAGGKPVDIEDGGYECVAPSAIAFEGYPVPRELNLADITKLKLDFAAA
                     AERALSADFDLIEIHSAHGYLLHEFLSPISNKRSDEYGGDFDNRIRLLCEIASELRLI
                     VPSSRALTVRISATDWIEGGWDLDQSIELVRELKARGVDLVDVSSAGISMAQQVKLGP
                     AYQTEFAEKIHQETGMATCAVGSITTGPQAEEILQRGNVEAVMIGRAMLANPRWPAQA
                     ALDLGEELPLPNQYLRAVFN"
     gene            complement(5785..>6459)
                     /locus_tag="AC16Y1_02520"
     CDS             complement(5785..>6459)
                     /locus_tag="AC16Y1_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5831370.1"
                     /translation="TFAFIEMVIPFGALGFAEQQLSSSLTGLLVAAVPIVNAVITYQM
                     GLDRNWNTKRVSGLLIGLFGVALLVGFEIRAENWWSVAIVAFAVLGYALGPVIISQML
                     SDVPSSGTIAWSQFFAMLFYLPIVAYQFTNGSWKVVDQVSNNAILSVVSLGLLCTAIA
                     FLVLFKAIDEVGPSRITLITYINPAVAVVLGILILAEPLTAGMILAFPIILIGSILAA
                     SKAKTN"
BASE COUNT         1860 a         1402 c         1511 g         1686 t
ORIGIN      
        1 atagcggtcg aatgtaggcc ggtatgaaac cggggaagtc aaaggcacgg tcatagccac
       61 cttttcgtgc ttcgtcacga atcgaatttc cgtagtcgaa tacctcagcg cctttatcca
      121 taaaaccaac catggcttca acatgctttg ccatagcttc acgtgatcga tcagtaaatt
      181 catctggttt tttatcggcg tattcacgag cttggtcaaa ttcaattcca gttgggatgt
      241 aatacagcgg atcgtgggct gatgtttgat cagtgacaat gtctaccggc acatccaatt
      301 ctaaaagctt tggcaaaact tcagcggcgt tgccaactaa tccaacggag agtccccgtc
      361 tttcgttctt tgcttttaga cagcgatcta ctgcgtcttt taagtcagtg gttacctcgt
      421 ctaagtatct ggtctcaacg cggcgacgta gtcgagtttc atcgatgtcg ataattaaac
      481 aaacgccttc attcatagtt acggcaagtg gttgagcgcc acccatgcca ccgcaaccac
      541 ccgtcacagt taatgtgccg gctaaagtgc caccaaaacg ttttgcagcg acagccgcaa
      601 aggtttcata ggtaccttgc aaaattccct gggtgccgat gtagatccaa gaaccagccg
      661 tcatctggcc atacatagtt aaaccgagtt gttctaagcg gcggaattct ggccagttag
      721 cccaatctcc aactaaattc gaattcgcca aaataactcg aggagcccat tcgtgagttt
      781 gaaaaacacc aactggtttt ccagattgaa ccagcatggt ttcatcattt tctaaattgg
      841 taagtgtgcg cactattgca tcaaaagagg cccagtttct cgcggcttta ccagtaccgc
      901 cataaaccac aagttcgtca gggtgttcgg caacattggg atctaaatta ttcatcaaca
      961 tccgaattgc agcctcttgc tgccaacctt ttgctgacaa tttagttccg gtagcagctt
     1021 ttactggacg tggacctgac aagcgaaaca ccataccttt aagaattatc cggatcgttg
     1081 attctattta ttagtgaaat caattattca acgctagccc aaatccttca tagttaatac
     1141 cccatatttt gacttggcca cctgtcgccg tagtcgaaat tacctattac cgataagttt
     1201 tattaacgaa ccccagaaag cttggaaatg actaatctgc ctagtgatcc gttatggcca
     1261 cgtgccggtc actggctttc aacagaagca caaagtaatc ctgaccttat ttatttaggt
     1321 gtgccagcac acttaacttc aatcagtcca actaatgcac agacgactcc agatgcagtt
     1381 cgcgacgcgc tactgcggta ctcgacttat tcttggtcaa aaaatttaga tgtaacccga
     1441 tgtaattttt ttgacgcggg taacgtagcc gatccggatt atgcagaagg cgcagtgcgg
     1501 gtagcgcaga agttggcgca gttgccagcc actgagttac ttattgctgt tggtggtgat
     1561 aattcaatta ctcattcggt tgcgaaatca ttctggaaag aaaatattgg gaaagctggt
     1621 ttgattactt ttgatgcaca ccatgatttg cgggatggcg aaagtaacgg atcgcctgta
     1681 cgccaactaa ttgcagctgg actccaaggt acacatgtgg tgcaaattgg aataagtgac
     1741 tttgcaaact caccagcgta cgctaaacga gccaaagagt atggcatcac agttatcact
     1801 cgagatgcaa tgcgcaggcg acctctttcg gaaattatgc aagaagcaat cgaaattgcg
     1861 ggggctagcg ggggaccgat tcacgtggac gttgatgtcg acgtttgcga tcgctcagtg
     1921 gttccagcat gtccagcagc agccccgggt ggaataagtg cagatgaatt gcgccaactt
     1981 gtccacttag cctgtctgaa cacgagcgta aagtccattg atttcaccga gatagatgcg
     2041 agtatcgata gcccggatca gcgcaccgtt cggctcgctg cgctttgtat tttagaagca
     2101 gctgcagggg tagtaattcg cactggaaaa ttagaagatt agtcaagtga aaaaaaagga
     2161 gatagtaact gatggccaca ttaaagaact tcatcggcgg caaattagtt gatccaatca
     2221 agggtcaaat gatggacatg gaaaatcctg ctaccggaca aatttatgga catgcaccaa
     2281 agtcatctcc agaagatgtt gatttagcat tcaaagctgc tgacgcgtcg ttcgatgcat
     2341 ggaagcgaac tagtccagca gatcgcagtt tggcactatt ccgcattgca gatgcgatgg
     2401 aaaaaagagc ttcagaaatt attgctgcag aagttcaaga cactggcaaa ccagtctggt
     2461 ggatgcgcga catggaaatg ccaatgatcg ttgaccacac tcggttccat gccacgcttg
     2521 ctaggaactt acctggatta tcgactggcg aatatttacc tggctacgac tcaacagttc
     2581 gccgcgaacc agttggtgtt tgcgcacaag ttgctccgtg gaattaccca atgttgatgg
     2641 cggtttggaa atttgcaccg gcaatcgccc ttggtaacac aattgtgtta aagccttcag
     2701 atacaactcc agcgtcagct gcaatcttgg cagaaatatt tgctgaacat ttaccagcag
     2761 gtgtggccaa cattatttat ggtgatcggg atactggtcg cgcaatggtt gcacacaaac
     2821 gtcccgatat ggtttcgatt actggttcag tgcgtgctgg tatggaaatc gccgcaaatg
     2881 ccgctccaga cttaaagcga gttcacttag aacttggtgg caaggctcca gttattgttt
     2941 ttgctgattg tgactttgat cgaactgtcg atgaagtcgc aactgcaaat gttatgaatg
     3001 ctggacaaga ctgtgctgct ggctgtcgcg taattgttga agaaagtatt gctgatgaat
     3061 tcaccagagc actagctaaa cgtatcagcg agtttaactt tggtgcccct gaagaggata
     3121 agaattacta cggctcactt aatagccaag cccagttgga tcgcgtacaa ggctttatag
     3181 atcgcctacc aagtcacgcg caaatactta ctggcggcaa ggcgtcacgc ctaaacggtg
     3241 ggtactactt tgaaccaact ctggtagctg gcttaaagca ggatgacgaa atgattcaaa
     3301 atgaagtgtt tgcatctgtg caaactattc agacatttaa agatgaagca gacgcaatcc
     3361 ggatggccaa cgatgtcgct tacggtttag cagcctctat ttggtcaacc aatcatcaga
     3421 agatcattcg cgttagccaa gatatcgact tcggtcaggt ttgggttaat tgccacttag
     3481 ttcaagttgg tgaaatgcca aatggtggtt tcaagcactc aggccacggt aacgatttat
     3541 caaatgaggc gattgaaggc tatacccggg taaagcatgt aatgagctac accggtcgct
     3601 agtcgattaa taattaaagc aaggcgttgg atttccagcg ccttgcttga tttttgtctc
     3661 atttattctt cgctaggctg gaacttcaat aacttaaagg tcactaaatg gatttgcgta
     3721 agcgggattt cttaaaagaa atcgatttta ctgctgccga atttcagtac ttgattgact
     3781 tagctatcga attgaagcgc gagaaaaaag cgcgtaaaga aaaacaacgc atggttggcc
     3841 gcagcgtcgc attaattttt gaaaaaccat ccactcgaac aagatgtgct tttgaggtgg
     3901 ctgcttttga tcaaggtgca tttactacct acttggatgg atcatcctct caagtcggtc
     3961 acaaagaatc agtggctgat actgctcgag ttttatctcg ctattacgac gcaattgaat
     4021 accgaggcaa cgagcaggag ttagttgagg agttagctga gtattctgat gtcccggttt
     4081 ataacggact aactgatcaa tggcatccaa cacaaatgtt ggctgatttt atgaccatga
     4141 tcgaacattc aggtaagcgg ccagaagaaa ttaagttcac ctacgtcgga gacgcaagat
     4201 ctaacatggg caattcactt ttagtcacca gcgcattact tggtagcgat attcgaattg
     4261 cttctccaaa aaagttgtgg cctagtcaac aaattcagga tttagcaaca aatttagcca
     4321 aaacaagtgg cgcaaaactt ttactaactg aaagtatggc ggacgcatta cctggctctg
     4381 actttgtgca cacagatgta tgggtttcaa tgggcgagtc agaagagatt tggaaagaac
     4441 gggttgagtt actgggtgac taccaagtta actatgagac tttagctata acacaaaatc
     4501 caaatgccaa gttcatgcat tgcttacctg gattccataa tgagcaaaca acagttggcc
     4561 gagctatcgc aaataaaact ggaatgcatg agggcttaga agtatcgcac gacgtatttg
     4621 aaggcccgca aagtgtagtt tttgatcaag cagaaaatag actccacacg ataaaagcaa
     4681 ttatggtcgc cacaatcggt gactaattca gggaaaagaa aaataaattg agcaagttat
     4741 ttagtccaat taaagttggt ggcatcgaaa ttaaaaaccg tgcatgggtt tcacctatgt
     4801 gtcagtactc ggcagtgaat ggtttaattc aagactggca tgccgttcac tacggagcgt
     4861 ttataactgg tggcgctgga ctagtaatgg ttgaagcaac tggtgttcaa gaaatcggtc
     4921 gaatcacccc gggctgtgtt ggtatttgga cagatcaaca agcgcaagtt cttgctgaag
     4981 ttccgagatt tgcacataga tacggcgtta aagcaggaat ccagcttgct catgccggac
     5041 gaaaaggatc aacagcagcg ccatgggccg gtggtaaacc tgttgatatc gaggatggtg
     5101 gttatgaatg cgttgcacct tcagcaattg cattcgaggg atacccagtt ccgcgcgaat
     5161 tgaacttagc cgacataact aagttgaagt tagattttgc ggctgctgcc gagcgagcgt
     5221 tgtccgcaga ttttgatcta atcgaaatac atagcgcaca tggctattta ttacatgaat
     5281 tcttatcacc gatttctaat aagcgcagcg atgaatatgg tggtgacttt gataatcgaa
     5341 tccgattatt gtgcgaaatc gctagcgaac ttcggttaat agttccatcg tcgcgagcat
     5401 tgacagttcg catatccgcc actgactgga tagaaggagg ctgggattta gatcaaagta
     5461 ttgaattagt tcgtgaactt aaagcaagag gagtggactt agttgatgtt tcatctgctg
     5521 gtatttcaat ggcgcagcaa gtgaaattag gaccggctta tcaaacagaa ttcgctgaaa
     5581 aaatccatca agaaactggc atggcaactt gtgcagttgg ttcaataacc acggggccac
     5641 aagctgaaga aattttgcaa cgtgggaatg ttgaagcggt aatgattgga cgtgcgatgc
     5701 tcgcaaatcc acgctggcca gctcaagctg cacttgatct gggcgaagaa ttacccctgc
     5761 cgaaccaata cttaagagct gtctttaatt agtttttgct ttactcgcag ccaggattga
     5821 gccaatcaag ataattggga aagccaggat cattcccgca gttaatggct cggccagaat
     5881 taaaatacct aaaactacgg cgactgctgg atttatgtaa gtgattaaag tgatccgact
     5941 tggtccaact tcatcaatag ctttaaataa gactaagaag gctatggcgg tacaaagtag
     6001 gcccaggcta accacgctaa ggattgcgtt attggaaact tggtcaacta ctttccaact
     6061 gccattagta aattgatagg ccacaatcgg caaataaaat aacattgcaa aaaactgaga
     6121 ccaagcgatt gttccgctac ttggcacatc acttagcatt tgcgaaataa ttactggacc
     6181 aagtgcgtag ccaagtacag caaaagcaac aattgcgacc gaccaccagt tttcagcacg
     6241 gatttcgaag ccgactaaaa gtgcgacacc aaatagacca atgagcaaac ccgagacgcg
     6301 cttggtattc cagtttcgat ctaaacccat ttggtaggta atcacagcat ttacaatcgg
     6361 caccgcagct actaacaagc cagtaagtga gctggaaagt tgttgctcag caaaaccaag
     6421 ggcgccaaat ggaataacca tctcgatgaa cgcgaaagt
//
LOCUS       DBNHVN010000137         3170 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1066261, whole genome shotgun sequence.
ACCESSION   DBNHVN010000137 DBNHVN010000000
VERSION     DBNHVN010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1066261"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="AC16Y1_02525"
     CDS             <1..823
                     /locus_tag="AC16Y1_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR5831371.1"
                     /translation="ALALMRKGDDVQFRARFSTDKDGDFLYQEALKNQLDVTYSVRTD
                     QPATLVEVRLSATGSPSYEFHLSGTADWHWTDAELAELDLGSQDAVIYGSLAAILEPN
                     FSRIQEWLRSHNNSNLLVGYDPNARPSAIDQRDHDVIRDRIIQLVRSADVVKVSDEDL
                     AWISPEVDPESMASYWSTTGPQLVILTRGENGASAYRQGNCLAHASGVKTKVADTVGA
                     GDTLMAWLVSGLIDSALICRFDADNVIRILDQAVKAAAITCSRVGCDPPNYAEVN"
     gene            complement(816..1295)
                     /locus_tag="AC16Y1_02530"
     CDS             complement(816..1295)
                     /locus_tag="AC16Y1_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HIR5831372.1"
                     /translation="MVEVEVVGIRVELPANQPVLVLRDRAGDRYLPLWIGSSEATAIS
                     LALDEVAPPRPLTHDLLVNVIDHLDDQVMSASITELIDGVYYAQLDFANHDAVTARPS
                     DAVAIALRCGVPIYVAQEVFDEAGMDVVTDDEDLDNPEEEVEKFRAFLDEINPEDFS"
     gene            complement(1318..2040)
                     /gene="ftsR"
                     /locus_tag="AC16Y1_02535"
     CDS             complement(1318..2040)
                     /gene="ftsR"
                     /locus_tag="AC16Y1_02535"
                     /inference="COORDINATES: protein motif:HMM:NF050798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FtsR"
                     /protein_id="HIR5831373.1"
                     /translation="MADKAYASHSSLKSIGEVITLLRDEFPDISISKIRFLESHGLLT
                     PQRTESGYRKFSVVDVENLRYILRMQRDYFLPLRVIREHIDAMNRGLEPPTVGDVAPK
                     PPKVIVDVDEITNNGATAIRLTKAELIANCSDDEKFVNELIDFGIIKADSSGYFTGTS
                     LNIAQKCLTLAQFGLGPRHLKTVVSSASRELDLFEPILKNARSGKNPMSKAQAEDLAL
                     QLAGTIVSLHAYVVRDLLNLEN"
     gene            complement(2040..2507)
                     /locus_tag="AC16Y1_02540"
     CDS             complement(2040..2507)
                     /locus_tag="AC16Y1_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018522497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR5831374.1"
                     /translation="MKCSACGQDNQQQGQPCSNCGVVFSLFEATGVIPLTEDDSAELG
                     IPTDLPSVASGGAALVVRKGPLEGVRFDLVAKDGQIISVGRSPESSIFLDDVTVSRKH
                     ATLARVNGVWIISDTGSLNGTYVNRDRVESGALKNGDELQIGKYRFAFVTGDI"
     gene            complement(2519..2908)
                     /gene="gcvH"
                     /locus_tag="AC16Y1_02545"
     CDS             complement(2519..2908)
                     /gene="gcvH"
                     /locus_tag="AC16Y1_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR5831375.1"
                     /translation="MTNIPADLNYTEEHEWVKVVDATTIRFGITDYAQDALGDIVFAD
                     LPKAGTAISAGSPCGEVESTKSVSEIYAPVDGTIAAANSAVETAPETLNTDPYGNGWL
                     ADVTVGDANAAVAGLLDAAGYRAVIGE"
     gene            complement(2932..>3170)
                     /locus_tag="AC16Y1_02550"
     CDS             complement(2932..>3170)
                     /locus_tag="AC16Y1_02550"
                     /inference="COORDINATES: protein motif:HMM:NF017734.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HIR5831376.1"
                     /translation="VRIVANTWFADATTGVSVSGTTLTLPIRISVIGDRTVLAPALEI
                     PGGLVDTVTTGGGTVSLEEIENISIKSVVPLATR"
BASE COUNT          871 a          737 c          687 g          875 t
ORIGIN      
        1 cgctttagca ttgatgcgaa aaggtgatga tgttcaattt cgagctcgat ttagcacgga
       61 taaagatggc gattttctct atcaagaagc attaaagaat caactagatg taacttatag
      121 tgttcgcact gatcagcctg caactttagt tgaagtaagg ctctccgcca ctggatctcc
      181 cagctatgaa tttcatttat ctggtactgc tgattggcac tggaccgatg cagaattggc
      241 agaacttgat ttaggttcac aagacgcggt tatttatgga tcgctagcgg caattctaga
      301 acctaatttt tctcggattc aggaatggtt aaggtctcac aataattcga atttactagt
      361 tggctacgat ccaaatgcgc ggccaagtgc tattgatcaa agggatcatg atgtaattcg
      421 agatcgaatt attcaattag ttcgctctgc agatgttgtt aaagtttcag atgaagatct
      481 tgcgtggatt tcaccagaag tagatccaga atcaatggca tcttattgga gcactactgg
      541 accacaatta gttattttga ctcgcggtga aaacggcgca agtgcatatc gccagggtaa
      601 ttgtctggcc catgcaagtg gggtcaagac aaaagttgct gacacagttg gtgctggcga
      661 cacacttatg gcttggttag tttctggatt aatcgacagc gcactgattt gcagatttga
      721 tgctgataac gtaataagaa ttttggatca ggccgtgaaa gctgcggcaa tcacttgtag
      781 tcgagtaggt tgtgatccgc ctaattacgc tgaggttaac taaagtcctc aggatttatt
      841 tcatctagaa aggccctgaa tttttcaact tcttcttcag gattgtctag atcctcatca
      901 tcagtaacta catccatgcc ggcctcatca aatacctctt gggctacata aatgggtact
      961 ccgcatctaa gcgcaattgc caccgcatca gacggacgag ccgtaactgc atcgtgattc
     1021 gcaaagtcca attgcgcata gtaaacccca tcgattaatt cggtaatcga ggcggacata
     1081 acttgatcat caagatgatc gattacgtta acaagaagat catgagttag tggtcttggt
     1141 ggtgcgactt cgtctaaagc caatgaaatt gcggtcgcct cagaacttcc tatccaaagt
     1201 ggcaagtaac ggtccccagc gcgatctcgc aggactagaa ccggctgatt agcgggtaat
     1261 tcaactcgga ttccaacaac ttcaacttca accacgttaa aagtgtatgc gttgagttta
     1321 gttttcgagg ttcaaaagat cgcgaacaac gtacgcgtgt agcgaaacga tcgtacctgc
     1381 tagctgcaga gccaaatcct cagcctgagc cttagacatt ggatttttac cacttctggc
     1441 attttttaga attggttcaa ataaatccag ttctcttgag gcgctggaaa ccacggtctt
     1501 taaatgacgt ggacccaagc cgaactgcgc caaggttaaa catttttgtg cgatgttcag
     1561 gcttgtacca gtgaaataac cagatgaatc agctttaatg attccaaaat caattagctc
     1621 attcacaaac ttttcatcgt cggaacaatt cgcgataagt tctgccttcg tcagtcgaat
     1681 tgcagttgcg ccattgttgg tgatttcatc tacgtcaact atgactttag gaggcttagg
     1741 cgccacatct ccaaccgtgg gaggttctag tccccgattc atggcatcga tgtgttcgcg
     1801 gattactcga agtggcagaa aataatcccg ttgcatccgc aggatgtatc gcaaattctc
     1861 tacatcaact acagaaaact tacgataacc actttcggtt ctttgtggag taagtaaacc
     1921 gtgcgattca agaaaacgaa tctttgaaat cgagatgtct ggaaactcat ctcgcaataa
     1981 agtgatgact tccccgatac tcttcaacga gctgtgggaa gcataagcct tatcagccac
     2041 taaatgtcac ctgttacaaa tgcaaatctg tatttgccaa tttgcaactc gtcgccgttt
     2101 ttgagtgcgc cgctttcaac tcgatctcgg ttgacatacg taccgttcaa tgagccggta
     2161 tctgaaatga tccaaactcc attgactcgc gcaagagttg catgcttgcg agatactgtc
     2221 acatcatcaa gaaaaatact gctctcaggt gatcgaccta ctgaaatgat ctggccatct
     2281 ttagcaacta aatcaaaacg aacgccttca agtggacctt ttcgaacaac taaagctgcg
     2341 ccaccagaag caacactcgg caaatctgtg ggaattccta attcggcact atcgtcttcc
     2401 gttaacggaa taacgccagt tgcctcaaac aacgaaaata caacaccaca gtttgagcaa
     2461 ggctgccctt gttgttggtt gtcttgacca caagctgagc atttcatttt tgtggttgtt
     2521 actccccgat tactgcgcgg tatccagcag catcaagtaa acctgctacc gcagcatttg
     2581 cgtcaccaac ggtcacatcc gctaaccagc catttccgta cggatctgta ttaagtgttt
     2641 ctggagcagt ctcaactgca ctatttgccg ctgcgatcgt gccatctact ggtgcgtaaa
     2701 tttcgctcac actttttgtg gactcaactt caccacatgg cgatccagcg cttattgccg
     2761 tacctgcttt tggtaaatca gcaaaaacaa tgtcgccaag tgcgtcttga gcataatctg
     2821 taattccaaa acgaatggtt gttgcgtcaa caactttaac ccattcatgc tcctcggtgt
     2881 aatttaaatc tgccggaatg tttgtcatag caaaagaata ctgcaaaact gttaacgagt
     2941 ggctagaggg accactgatt taattgaaat attttcaatt tcttcaaggg acacggtgcc
     3001 gcctcccgtg gtcacggtat caactaatcc acctgggatc tcaagggcag gcgccaagac
     3061 cgtccgatcc ccgataactg aaatgcgaat gggcagcgta agcgttgtcc ctgagacgct
     3121 caccccagta gttgcgtctg cgaaccaagt gttagcaact attcgaacat
//
LOCUS       DBNHVN010000138         1713 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_2032058, whole genome shotgun sequence.
ACCESSION   DBNHVN010000138 DBNHVN010000000
VERSION     DBNHVN010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_2032058"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="AC16Y1_02555"
     CDS             complement(<1..343)
                     /locus_tag="AC16Y1_02555"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RBBP9/YdeN family alpha/beta hydrolase"
                     /protein_id="HIR5831377.1"
                     /translation="MNPKRVLIIHGWGNRRPFGHWHRYLANTLRAQGHVVAYPQLPNT
                     DFPVFEEWLEVLKTELDMLGEVNNNELVVVAHSLGCLTWIKLAMHGLAPQDVSRVLLV
                     APADPTLCGEVP"
     gene            397..690
                     /locus_tag="AC16Y1_02560"
     CDS             397..690
                     /locus_tag="AC16Y1_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018207358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HIR5831378.1"
                     /translation="MAQYQITYWKDLPSMVVAKLGEETVKIQLAQRLQEAIDEAAMRL
                     GESDADAYLAGWSRSEWQEVDGDPKTVAEAIGAKLENEFTEEKIKTFLDQLSS"
     gene            699..1580
                     /locus_tag="AC16Y1_02565"
     CDS             699..1580
                     /locus_tag="AC16Y1_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018207357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR5831379.1"
                     /translation="MANLYEWLDAGNPLVGDGAMGTALQLLGLTDGGAPELWNVEQPE
                     KVSSILESYAAAGANLLTTNTFGGTKPRLQMHNLEDRVHELNKAGAEVARKIADRYEN
                     VFVLGDVGPTGELMEPMGTLTHADAKEIFAEQIKGLVDGGCDAILIETMSDLAEVSAA
                     VEAVQEVAPGMPVVATLSFDTNLRTMMGVKPAQAVTEISAMGVRLVGANCGRGTDEMR
                     IIAKELVEAKPAGVYVFLQSNAGLPQLVGSEFVYTGTSDEMAEFAAEMKELGINAIGA
                     CCGSTGEHIAAIAKVLN"
BASE COUNT          500 a          344 c          422 g          447 t
ORIGIN      
        1 ttggcacttc cccacaaagt gtcggatcag ctggggcaac taaaagcacg cgactcacat
       61 cctgtggcgc tagtccgtgc atggctaatt tgatccaggt taaacaacct aacgagtggg
      121 caactacaac taactcgttg ttgttcactt cacccaacat gtctagttca gttttaagaa
      181 cttctaacca ttcttcaaaa actggaaaat ctgtattagg taattgtggg taagcaacaa
      241 cgtggccttg ggcgcgaagt gtgtttgcta aatacctatg ccaatgaccg aatggtcggc
      301 gattacccca accatgaatg attagcactc gtttcggatt catcactgac gaccttagca
      361 gtgtgcgtaa cgataaaaca cccgtaaaat caacgtatgg cacagtacca aatcacttac
      421 tggaaagatc tcccatcgat ggtggtggca aagcttggtg aggaaacagt aaaaatccaa
      481 ttggctcagc gcttacaaga agctatcgat gaagccgcta tgcgccttgg cgaatctgat
      541 gcagatgctt acctcgccgg atggagtaga agcgagtggc aagaagttga tggtgaccca
      601 aaaactgttg cggaagccat aggtgccaaa cttgaaaatg aatttaccga agaaaaaatt
      661 aaaacttttc tagatcaatt aagtagttag ggcttgcgat ggcaaatctt tatgaatggt
      721 tagatgccgg aaacccatta gttggcgatg gtgccatggg tactgcatta caactacttg
      781 gtttaacaga tggtggcgca cctgaacttt ggaatgtgga acagccagaa aaagtgagtt
      841 caattcttga aagctacgct gcagcgggtg caaacctttt gactacaaat actttcggtg
      901 gaactaaacc acgtttacaa atgcacaact tagaagatcg cgttcatgaa ttaaataaag
      961 cgggggctga agtagcgcgc aaaattgcag atagatacga aaatgttttt gtattgggcg
     1021 atgtcggtcc aactggtgaa ttaatggaac caatgggcac gttaacccat gcagatgcaa
     1081 aagaaatttt tgctgaacaa attaaaggtt tagtcgatgg tggatgtgac gcaatcctga
     1141 ttgaaactat gagtgactta gccgaagtaa gtgctgcagt tgaagcagta caagaagttg
     1201 cgccaggaat gccagttgtt gctactttga gttttgatac caacttgcgc accatgatgg
     1261 gtgtgaaacc tgcccaagca gtaaccgaaa taagtgcgat gggtgttcgg cttgttggcg
     1321 ctaactgcgg tcgcggcaca gatgaaatgc gaataattgc caaagaatta gttgaggcaa
     1381 agccagctgg tgtttatgta tttttgcaat caaatgcggg gctacctcaa ttagttggtt
     1441 cagaatttgt ttacaccggc acatccgacg agatggcaga gtttgctgct gaaatgaaag
     1501 aacttggcat caatgcaatc ggggcctgct gtggttcaac aggtgaacat atagcagcaa
     1561 ttgcaaaagt tcttaattag tttgcgggtt tattcttgac tgattctttt agcgatgacg
     1621 attcgcatcg cgacgcacta ggtccgggcg aattttatcc agttgttggc gttggccctc
     1681 atccaaaacc gtggccaact gatttacatt ttg
//
LOCUS       DBNHVN010000139         1303 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_693894, whole genome shotgun sequence.
ACCESSION   DBNHVN010000139 DBNHVN010000000
VERSION     DBNHVN010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_693894"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1303)
                     /locus_tag="AC16Y1_02570"
     CDS             complement(<1..>1303)
                     /locus_tag="AC16Y1_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019884837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HIR5831380.1"
                     /translation="SDVSLRVIADHARTCAFLIADGVLPGNEGRGYVLRRILRRVVRN
                     MRILGANDPTIAELIDASITAMTAQYPELKTDAGRITSIASAEEESFLATLERGTALF
                     DLAVTDLKKDKKTALAGNTAFTLHDTFGFPFDLTLEMANEQGISVDETGFKELMQEQR
                     SRAKADAQARKSGVAAVTVYREIMNKSGVTDFTGYRETESESEIRGILVNGVDSSSAK
                     TGDEVEIVLDRSSFYAESGGQLADHGLIEGSNGALIEIVDVQTPVPGLFVHRGKVKSG
                     DFNIGLKAISKVDLKRRTAISRAHTATHMVHKAFREALGETATQMGSENSPGRFRFDF
                     PSSGAVPQSVLTDVQDRVNEMLIQDLSVTASVMSQEEARKTGAMALFGEKYGDEVRVI
                     SIGDWARELCGGTHTQTTGQLGIVTFLSESSIGAGVRRVEAL"
BASE COUNT          327 a          339 c          270 g          367 t
ORIGIN      
        1 agcgcttcaa ctctgcgcac gcctgcgcca atcgagcttt ccgaaagaaa tgtcacgata
       61 cccaattgac ctgtcgtctg tgtatgcgtt ccaccacaaa gttctcgagc ccagtcacca
      121 atactgatga ctcttacctc atctccgtat ttctcgccaa aaagtgccat cgctccagtt
      181 ttgcgagctt cctcttggct catcacggat gctgtaacgg aaagatcttg aatcaacatt
      241 tcattaacgc gatcttggac atccgttagt actgactgag gaacagcacc gctacttgga
      301 aaatcaaatc tgaatcggcc tggactattt tcagagccca tttgagtcgc agtttcgccc
      361 agtgcttcgc ggaaagcttt gtgcaccatg tgcgttgcag tgtgagctct cgagattgcg
      421 gtgcgtcgct ttaagtcaac tttagaaatt gctttcaaac caatgttgaa gtcgccggat
      481 ttaacttttc cgcgatggac aaatagacct ggtacaggtg tttgcacatc aacaatttcg
      541 atgagtgctc cgttagaccc ttcaattagg ccatgatctg caagttgacc gccactttcg
      601 gcgtaaaatg aagatcgatc tagaacgatt tcaacttcgt cgccagtctt tgccgaggaa
      661 ctatcaacgc cattaactaa aatcccccga atttcacttt ctgattccgt ttcacgatat
      721 ccagtgaaat ccgtcacgcc acttttattc atgatctcgc gataaacagt aacggcggct
      781 accccgctct ttcttgcttg agcgtcggcc tttgctcttg atcgttgttc ttgcattaac
      841 tctttaaagc cggtttcgtc tactgaaata ccctgctcat ttgccatttc aagcgttaag
      901 tcgaagggaa aaccaaatgt gtcatgcaaa gtgaacgcag tattcccagc aagtgcagtc
      961 tttttatctt ttttaagatc ggtaacagcc aagtcaaata acgcagtacc gcgctcgagt
     1021 gtagccagga atgattcttc ttcagcgcta gctattgatg ttattcggcc ggcatccgtt
     1081 tttagttctg ggtactgagc agtcatcgct gtgatacttg catcaatcaa ctcagcgata
     1141 gttggatcat tcgcaccaag tatgcgcatg ttccgaacca ctctgcgcaa gattcgacgc
     1201 agtacgtagc cacgaccttc gttcccaggc agaacaccat cagcaattaa aaatgcacaa
     1261 gtgcgagcgt ggtctgcaat aactcgcaaa cttacatctg att
//
LOCUS       DBNHVN010000140         1028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_323699, whole genome shotgun sequence.
ACCESSION   DBNHVN010000140 DBNHVN010000000
VERSION     DBNHVN010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_323699"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1028
                     /locus_tag="AC16Y1_02575"
     CDS             <1..>1028
                     /locus_tag="AC16Y1_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase family
                     protein"
                     /protein_id="HIR5831381.1"
                     /translation="STENVPSRDELISSLLQAVSQTKTWDDGLSQLRISYRKELSAIA
                     AFDVFAKENSISILPGIATALSDLADAVIEAALYLSRKHTEQSEKVELAVIAMGKCGA
                     RELNYVSDVDVMFILQPSANGVDEAVAIEIATQIAKKLMQACSTSTNEGAIWEVDAAL
                     RPEGKAGSLVRTLQSYLDYYDRWAQSWEFQALLKARPMAGSIELGEQFISAIHPYIWQ
                     AANRPGFIEDVQAMRERVTENIPSKDAQRELKLGRGGLRDVEFAVQLLQMVHGRSDDL
                     VRSPNTLLALEQLATWGYVAREDAATLSAAYRFLRTLEHRIQLFQMRRTHIVPTDDGR
                     QRQIGRSM"
BASE COUNT          280 a          224 c          259 g          265 t
ORIGIN      
        1 ctagtaccga aaatgtgccc agccgagatg aattaatttc ttcattgtta caagcggttt
       61 ctcagactaa aacttgggac gatggattaa gtcaactgcg aattagctac cgcaaagaat
      121 taagtgccat tgcagcattt gatgttttcg caaaagaaaa tagcatcagt attttgcccg
      181 gaattgccac cgcgctaagt gacttagctg atgctgttat cgaggctgcg ctctaccttt
      241 caagaaaaca taccgagcag agtgaaaaag ttgagctcgc agtaatcgca atgggtaagt
      301 gcggtgcacg ggaacttaat tatgtaagtg acgtagatgt gatgtttatc ttgcaaccct
      361 cggcaaacgg agtcgacgaa gcagttgcaa ttgagatagc cacccagata gcgaaaaagt
      421 taatgcaagc ctgctcaaca tcgaccaatg aaggcgcgat ttgggaagtt gatgcagcgc
      481 ttcgtccaga aggtaaggcc ggttcactgg tgcgcacctt acaaagttat ttagattatt
      541 acgatcgctg ggctcagtct tgggaatttc aagcgctact taaggcaaga cctatggctg
      601 gcagcataga gcttggcgaa caatttattt cggcgataca tccttacatc tggcaggcag
      661 cgaatcgccc aggttttatc gaagatgttc aggctatgcg ggagcgagtt actgaaaata
      721 ttccttccaa ggatgcgcag cgtgaattaa aactgggtcg cggtggcctg cgcgatgttg
      781 aattcgcagt tcaacttttg cagatggtgc atggtagatc agatgactta gttcgaagtc
      841 caaatacctt gcttgcacta gaacaacttg ctacttgggg ttatgttgcc cgtgaagatg
      901 cagcaacgct ttccgctgct tatcgatttc tgcgaaccct cgagcatcga atccagttat
      961 tccaaatgcg ccgaacccac attgtgccga ctgatgacgg taggcagcgt caaattggtc
     1021 gatccatg
//
LOCUS       DBNHVN010000141         1268 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1883935, whole genome shotgun sequence.
ACCESSION   DBNHVN010000141 DBNHVN010000000
VERSION     DBNHVN010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1883935"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="AC16Y1_02580"
     CDS             <1..901
                     /locus_tag="AC16Y1_02580"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013287432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5831382.1"
                     /translation="VDVMTALVSFDRVFEVLDLKPMVSDAPDAKELNRGPAKVELKNV
                     SFRYPLPEEVSLASLESIARTDLLASSDLILDGVNLEVKPGQMYALVGPSGAGKSTLT
                     ALVPRLFDPISGQVLINNVDLRDATQASIHEVVGVVTQDAHMFHDTIRTNLLYAKPQA
                     TEAQLWQACEDAQIASMIRELPEGLDTVVGDRGHRLSGGEKQRLAIARLLLKAPDVVI
                     LDEATAHLDSENEALVQEALSKALVGRTSIVIAHRLATVRQADQIVVLGEGKIVEQGT
                     HEELLNKNGLYADLYNRQFAPLD"
     gene            complement(869..>1268)
                     /locus_tag="AC16Y1_02585"
     CDS             complement(869..>1268)
                     /locus_tag="AC16Y1_02585"
                     /inference="COORDINATES: protein motif:HMM:NF014193.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HIR5831383.1"
                     /translation="VDGTKINVVRGWTAAGSSAQVSPPLSAVNTSQVELMGRLRQINP
                     PNLIEPTDIPVGQRIGLDPKYGDAYLELVSANPTLNNPEVLTLPGPKITEGTHRSYAI
                     QWQIFAVMLIIGYAILFRNDLIERRKLPVV"
BASE COUNT          346 a          257 c          302 g          363 t
ORIGIN      
        1 tgttgatgtc atgacggctc ttgtttcttt tgatcgcgta tttgaagttc ttgatttaaa
       61 gccaatggtc agtgatgcgc cagatgctaa agaactaaat cgtggaccag cgaaagttga
      121 acttaaaaat gtttcgttta gatacccgct acctgaggaa gtatcgcttg ccagcttaga
      181 aagtattgct cggacagatt tgttggctag ttctgatctc attttggatg gcgtcaactt
      241 ggaagtgaag cctggtcaga tgtatgcatt ggtgggtcca tctggagcag gcaagagcac
      301 actcactgcg ctcgttccca gactttttga tccaatttct ggtcaagtgt taataaataa
      361 tgtggatttg cgtgatgcta cgcaagcatc aattcacgaa gtagttggtg ttgtcacgca
      421 agatgcccac atgttccatg acaccattcg gacaaattta ctttatgcaa agcctcaggc
      481 aacagaagca caactttggc aagcctgtga agacgcccaa atcgctagca tgattcgaga
      541 gctgcctgaa gggctagaca cagttgtcgg agatcgcggt caccgattaa gtggtggtga
      601 aaagcagagg ttggctatcg ctcgattgtt acttaaagct cccgatgtgg tcattttgga
      661 cgaagcaacc gcacatctag atagcgaaaa tgaagcctta gttcaggaag cgttgagtaa
      721 agctttggtt ggtagaactt caattgtgat cgcccatcgt ctggctactg ttcgccaagc
      781 agatcaaata gttgttttag gtgaaggcaa aattgttgaa caagggactc acgaagagtt
      841 gctaaataaa aatggacttt atgctgattt atacaaccgg caatttgcgc cgctcgatta
      901 agtcgttcct aaataaaatc gcataaccga taatcaacat tacagcgaat atttgccact
      961 gaatggcgta cgaacggtga gttccttcgg tgattttagg accaggaaga gtaagtactt
     1021 ctggattatt aagtgttgga ttagcgctca ctaattccag ataagcatct ccgtattttg
     1081 gatctaatcc aatacgttgt ccgactggaa tatctgttgg ctcaattaaa tttggtggat
     1141 ttatttggcg caatcttccc attaactcga cctgggaagt attaactgca ctcaatggtg
     1201 ggctaacttg tgcactgctg ccggctgctg tccaaccacg aacaacatta attttggtgc
     1261 catcaacg
//
LOCUS       DBNHVN010000142         9505 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_694152, whole genome shotgun sequence.
ACCESSION   DBNHVN010000142 DBNHVN010000000
VERSION     DBNHVN010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 9505)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9505)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9505
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_694152"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="AC16Y1_02590"
     CDS             complement(<1..192)
                     /locus_tag="AC16Y1_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831384.1"
                     /translation="MSKRERNFWTLVVLSLILALVTWRNANNREGSLVVDPTNPAILT
                     SAQPEPPYDCAANNPFSEVN"
     gene            complement(441..513)
                     /locus_tag="AC16Y1_02595"
     tRNA            complement(441..513)
                     /locus_tag="AC16Y1_02595"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(478..480),aa:Arg,seq:ccg)
     gene            701..1318
                     /gene="pnuC"
                     /locus_tag="AC16Y1_02600"
     CDS             701..1318
                     /gene="pnuC"
                     /locus_tag="AC16Y1_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HIR5831385.1"
                     /translation="MFTFSDISDLALKTIFTIFGYEVSNLELVAVVTGFIGVWLGTTG
                     KKITWPWWGLSSLLYAWLFYEFELYASAALQFVFIAAAIWGWFGWAPTGAQPRTGTTK
                     QRLIVGAIAIGFWVIIAPALASLGAAATWPDSFGLVFSFVAQLIMVKQFTETWLIWLI
                     VDLVYTVLYATQGLWFTSALYLVFTFVAIRGWWQWGRKLTPAQHV"
     gene            1311..1868
                     /locus_tag="AC16Y1_02605"
     CDS             1311..1868
                     /locus_tag="AC16Y1_02605"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF024635.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase family protein"
                     /protein_id="HIR5831386.1"
                     /translation="MFDDSLINQLVSKVIAAGKFCGRTRVITIDGPAGSGKTTLAAKL
                     SSALSDCPVVHMDDLYSGWNQDLHVELAHRITTEILTPISKEKPAIYKKYDWADEKFK
                     LNEIVNVSDYLILEGVGAGHPEINNQAALNIWIEANAKTLLDRLVRRDGEHLREQLVK
                     WQTHEARYFEQLSVKGCADVVLSGD"
     gene            1941..5594
                     /locus_tag="AC16Y1_02610"
     CDS             1941..5594
                     /locus_tag="AC16Y1_02610"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HIR5831387.1"
                     /translation="MPNQPGSPFGPNDWLVDELYQQYLEDKRQVDPAWWDFFADYNPT
                     DYSAIKSTQVKPVAAAKVEPPVPAPAVQSAPSSSTAPVSQTPAAQPASAAPVISTTEP
                     EKLKGPAARVVTNMETSLAIPTATSVRAVPAKLMVDNRIVINNHLSRNRGGKVSFTHI
                     IGYAIVRALKDMPEMNVSYTTVEDKPAIVRHPNIGIGLAIDLAKEDGTRQLLVPCIKQ
                     ADTMDFAGFWAGYEDVVRKARTGKLAVEDFQGATISLTNPGTIGTVHSVPRLMQGQGV
                     IIGVGAMEYPAEWQGASEDTLTKNAISKVITLTSTYDHRVIQGAQSGDFLRRIHQYLL
                     GEGNFYDDIFRSLQIPYEPIRWASDIATSHDDELDKTARVQEMIHAFRVRGHLLADVD
                     PLEYQQRSHPDLDIANHGLTLWDLDREFATGGFGGKPFAKLRDILGILRDAYCRKVGI
                     EYMHLQDPEQRKWIQNQVERASDKMDRDEQLRVLRKLNEAEAFETFLQTKFVGQKRFS
                     LEGGESAIPLIDQVLIEAAQNDLDEICIGMPHRGRLNVLANIAGKEFSRIFNEFEGNY
                     GEESVQGSGDVKYHLGTSGEFTSPSGKKIGVYLAANPSHLEAVNPVLEGIVRAKQDII
                     GSAKKYSVLPILIHGDAAFAGQGVVAETLQFSQLQGYQVGGTFHLVINNQVGFTTSPR
                     YSRTSVYSTDVARMIQAPIFHVNGDDPEAVVRVAKLAFDFMQAFHKDVVIDMVCYRRR
                     GHNEADDPSLTQPLMYEIIDQKRSVRKLYTEALVGRGDITVEEAEGVLKHYQDELEKI
                     FQATKAENAEGQFSSHSAEVVQTGQQTLKPQTPTSAVDTSISREVIDRVVASQLNMPE
                     GFTIHPRLAPQLQRRKEMVDTDAIDWGMGEALAIGSLLTEGRSVRLAGQDSRRGTFGH
                     RHAVIVDKKTGWQFKPLKQCYEGNAKLWVYDSLLSEYAAMGFEYGYSVIRPDVLTMWE
                     AQFGDFANGAQTIIDEFIAAGEQKWAQRSRVTLLLPHGYEGQGPDHSSARIERFLQLC
                     AQDNITVAMPTTPASFFHLLRWQVHSPLDRPLVIFTPKSLLRAKYATSKVSDFTSGTF
                     TSIIGDKEANSAEVKTALLCSGKVYYDLVAHREKTGRKDVAIIRLERLYPLPAITLPP
                     ELARFENLQTVKWVQEEPANQGAWSFMAMNLPDVIGRQLVGVSRPASSSPAVGSHHRH
                     EIEQETLVNQAFS"
     gene            5602..5781
                     /locus_tag="AC16Y1_02615"
     CDS             5602..5781
                     /locus_tag="AC16Y1_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019889383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6104 family protein"
                     /protein_id="HIR5831388.1"
                     /translation="MYFTDRGIEELENRRGEEEISIAWLAARLQEFVDLNPDFEIPTE
                     RFATWLARLDDEVEE"
     gene            5781..6746
                     /locus_tag="AC16Y1_02620"
     CDS             5781..6746
                     /locus_tag="AC16Y1_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR5831389.1"
                     /translation="MLSIVATHGDAENPLAALSVTDLPEPEPIDNWVKVEIKAAALNH
                     HDIWTLRGQATVSENLPIVLGSDGAGITDQGMPVILHAVIGDPVNGDETLDPKRSLLS
                     EKYNGTFAKFTCVPKYNLIPKPEWLTFEQAACLPTAWLTAYRMLFSKSGLAKDDTVLI
                     QGASGGVASALIPLANAAGFRVWVTTRTESKKEYALKLGAHQIFDTNAKLPEKVDAVM
                     ETVGQATWAHSMRSLKPGGRIVVSGATTGANPPADLNRLFFLQLQIIGSTMGSKAELL
                     ELISFLEKTGLRPDIHKIYDFTHAKKAFEDLINGDVQGKLVLTPS"
     gene            complement(6732..7025)
                     /locus_tag="AC16Y1_02625"
     CDS             complement(6732..7025)
                     /locus_tag="AC16Y1_02625"
                     /inference="COORDINATES: protein motif:HMM:NF021981.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HIR5831390.1"
                     /translation="MALGIAIIEGLSMVPTYAPGERVIVKYGSAFQEGDVVLVDFKER
                     IDIKRIAKIENDRITIIGDNEAVSVDSRQYGQVKRTQIIAKVIYRLPNWLARR"
     gene            7150..7545
                     /gene="sodN"
                     /locus_tag="AC16Y1_02630"
     CDS             7150..7545
                     /gene="sodN"
                     /locus_tag="AC16Y1_02630"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HIR5831391.1"
                     /translation="MKFFAPTVRVFAHCDLPCGVYDPAQARIEAQSVKACMEKYHASD
                     DPNFKARAVAIKEERSHMVKDHLWVLWTDYFKAPHFEKYPQLHQLFNDATKLAGAAGT
                     KGTVDVAVADQLLSKIDEIANIFAETKKA"
     gene            7624..8103
                     /locus_tag="AC16Y1_02635"
     CDS             7624..8103
                     /locus_tag="AC16Y1_02635"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007382932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5831392.1"
                     /translation="MHSMIIELAEFEKAVDEVIATEEQLERALFGGTSHSAHSANTPH
                     GSPALFAHVIEDPEQPGQLAGMAIWFLNYSTWQGEHGIYLEDLYVRPKYRGKGLGKAL
                     LKKLAQVCNENGYTRFQWWVLTWNQSAIDVYKGIGAVPMDEWVVYRLTGDKLTEFSN"
     gene            complement(8100..8450)
                     /locus_tag="AC16Y1_02640"
     CDS             complement(8100..8450)
                     /locus_tag="AC16Y1_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831393.1"
                     /translation="MRRISYLIGLSQSLLLGGYAIAIFIIGVRLESTLGSPVVESIIY
                     LIFAAAIFFVSRGFARVQNWARTPYLVTQMFGLIVAYTLLAGDELNYRITGALVGLSS
                     AVAIYALVKTPLEN"
     gene            8482..9417
                     /locus_tag="AC16Y1_02645"
     CDS             8482..9417
                     /locus_tag="AC16Y1_02645"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012981.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR5831394.1"
                     /translation="MRSLLVVNSFATTTTPEVQAVITSALSNHLDLTVINTEDKSHGI
                     EIGKNAVKEGFELVIALGGDGTINELANGMLAKTESAEIPLLAGLPGGNANVFIRNLG
                     YSNDPVIATAQLIEKITQKSSRKIGLGKIDFAGNSRWFLFNAGLGLDARVLSRMDDKR
                     NQGKLASDFSYALITLKELFTEVRNGKAQLTITDQDGNTYSETQFALMINFSPWTYIG
                     KFAVSPTKKVMDTTSLDLFAPYELSVSRTAQLVRDLVLRSNLTNNENVLVLEKQKTLK
                     ISASVPTWIQTDGEALALVTAVSVKHFADCLTVIA"
BASE COUNT         2731 a         2083 c         2128 g         2563 t
ORIGIN      
        1 gttgacttct gaaaaaggat tattcgccgc gcaatcgtaa ggcggttcgg gttgcgctga
       61 agttaaaatt gccggatttg ttggatcaac aacaagtgac ccttcgcgat tgttagcgtt
      121 acgccaagta accagtgcca gaattaaact caacacaact agcgtccaaa aattacgctc
      181 acgtttggac atttttttag ggtaagttga ctgactcaca acacttaagt gtatgtggct
      241 gggtgtgcct cttttatttt cgcgctacta atgctgcgct tattgcacgt cgatccttac
      301 agtcctcagt gccctcgatg agcgtcgccg gctagcgcct gctccttcaa tcgagggctt
      361 gctggctcgc aaaagaattc attcttttgt tcgcgcagct tggtctgcgc ttattgcgct
      421 tcgatcccta cggtcctcag cgccctcgat tggattcgaa ccaacggcct tctgctccgg
      481 aggcagacgc tctatcccct gagctacgag ggccaacaac cactgagtgg cttagtaaac
      541 agactttacc agcaacaaat aactccactt acaaagcgca tttttaaagt tagacttaaa
      601 caaataacac gggagctcac acaggtgggc tgagagggta gcaattgcta ccgaccgtct
      661 aaccagtcgg gtaatgccga cttgaggagt aacgatcgat atgttcacct tttctgatat
      721 ttcggatcta gcgttaaaaa ctattttcac aatatttggt tatgaagttt caaaccttga
      781 acttgtcgct gttgttaccg gcttcattgg agtgtggcta gggacaactg gcaaaaaaat
      841 aacttggccg tggtggggat taagtagctt gctctacgca tggctatttt atgaatttga
      901 actttatgca agtgcagccc ttcaatttgt ttttattgct gccgctattt gggggtggtt
      961 tggctgggct ccaactggcg cgcaaccgcg caccggaact acaaaacaaa gattgatcgt
     1021 tggcgcaatt gcaattggtt tttgggtgat tattgcgccc gctttagctt ccttaggagc
     1081 tgcagcaacc tggcctgact cgtttggact tgtctttagc tttgttgcac aactgatcat
     1141 ggtgaaacag ttcactgaaa cttggttgat ctggttaatt gtggatttgg tctacacagt
     1201 tttatatgca actcaaggct tgtggttcac ttccgcactc tatttagtat ttacttttgt
     1261 tgctattcgg ggttggtggc aatggggtcg aaaactaaca cccgctcaac atgtttgatg
     1321 attccttaat taatcagtta gtttcaaaag taattgctgc aggtaaattc tgtggcagaa
     1381 ccagagtgat cacaattgat ggtccagccg gatctggaaa aaccacattg gcggcaaaac
     1441 taagttcagc ccttagcgat tgtccagttg tacatatgga tgatctgtac tcgggttgga
     1501 atcaagattt gcacgtcgaa ctcgctcatc gaataacgac tgaaatactc acgccgattt
     1561 caaaagagaa acccgcaatc tacaagaagt acgactgggc tgatgaaaaa tttaaactca
     1621 acgaaatagt taacgtaagc gactacttga tcctagaagg cgttggtgca ggacatccag
     1681 aaatcaacaa tcaggcagca ctcaatattt ggattgaagc aaatgcaaaa acattgcttg
     1741 atcgtttggt tagacgtgac ggtgagcatc ttcgtgagca attggttaag tggcaaaccc
     1801 atgaagcacg gtattttgag cagttatctg tcaaaggctg cgctgacgtt gtcttaagtg
     1861 gcgactaaat ttttcggttt tgtaaagccc cactaacctt gagttagtaa ttagacgtgg
     1921 aatctaggag gcgtggcata gtgccaaatc agcctggtag tccatttggt cctaatgact
     1981 ggttagtcga cgaactttac caacagtatt tagaagacaa acgacaagtt gatccagcat
     2041 ggtgggattt cttcgccgac tacaacccaa cggattactc ggcgatcaaa tcaacgcaag
     2101 ttaaacccgt tgccgctgca aaagtcgaac cgcctgttcc tgcgcctgcg gtacaaagtg
     2161 cgccatcaag ttcaactgca cctgtttcgc aaacacctgc ggctcaaccg gcatctgccg
     2221 ctccagtaat ttcaactacc gaaccagaaa agttaaaggg tccggcggct cgagttgtaa
     2281 caaatatgga aacaagtttg gctatcccaa ctgcaactag tgttcgagct gtcccagcaa
     2341 aactaatggt tgataaccga atcgtcatta ataatcacct ttcacgtaat cgtggtggca
     2401 aagtaagttt cacccacatc attggttacg cgattgttcg ggcactcaaa gatatgcctg
     2461 agatgaacgt ctcgtacaca actgtcgaag ataagccagc aattgttcga catccaaaca
     2521 tcgggatcgg cttagcgatt gatcttgcaa aagaagatgg cacacgccaa ttactagttc
     2581 cttgtatcaa acaagcagac actatggact ttgctggatt ctgggctggc tatgaagatg
     2641 tcgttcgaaa agctcgcact ggaaaactag ccgtcgaaga tttccaaggc gccacaataa
     2701 gtttgactaa ccctggaact atcggaacag ttcattcagt tccacgccta atgcagggtc
     2761 aaggcgtaat catcggcgtc ggagcaatgg aatatccagc tgaatggcaa ggcgcttccg
     2821 aagatacttt gactaaaaat gccataagta aagtgattac gctgacaagc acttacgatc
     2881 accgagtaat tcaaggtgca caatctggag atttcttacg tcgcattcac caatacctat
     2941 taggtgaggg aaatttttat gatgatattt tccgctcact gcaaattccg tatgaaccaa
     3001 ttcgttgggc gagtgacatt gccacctcac atgatgatga attagataaa actgctcggg
     3061 tccaagaaat gattcacgca tttcgggtac ggggacattt actcgccgac gttgatccac
     3121 tcgaatatca acagcgctcg catccagatc tagatattgc aaaccatggg ctaacacttt
     3181 gggatttaga tcgagaattt gcaactggtg gctttggtgg aaaacccttc gcaaaattac
     3241 gcgatatttt aggtattttg cgcgatgcct actgtcgtaa agtcggcatt gaatatatgc
     3301 atttgcaaga tcctgaacaa cgtaaatgga ttcaaaatca ggttgagcgg gcctccgaca
     3361 aaatggatcg cgatgagcag cttcgagtat tgcgtaaatt aaatgaagct gaagcttttg
     3421 aaactttctt gcaaacaaaa tttgttggcc aaaaacgttt cagcctagag ggtggcgaaa
     3481 gtgccattcc gctaattgat caggttctaa ttgaggcggc acaaaatgac ctcgatgaaa
     3541 tctgcattgg catgccacat cgcggtcgat taaatgtcct tgcaaatatt gctggtaaag
     3601 aattttcgcg aatcttcaat gaattcgagg gaaattacgg cgaagaatct gttcaaggct
     3661 ctggtgatgt gaaataccac ctgggtactt ctggtgaatt cacctcgcct tcaggtaaaa
     3721 aaattggtgt ctacttagca gccaacccat cacaccttga agcagtaaat ccagttcttg
     3781 aaggtatcgt tcgcgcgaag caagacatca ttggctcagc aaaaaaatac tcagtgctac
     3841 cgattttaat tcacggtgat gcagcatttg ctggacaagg tgtggtcgct gagacgctgc
     3901 aatttagtca gcttcagggc tatcaagttg gtggaacttt ccatttagta atcaataatc
     3961 aagttggctt cacgacatct ccgcggtata gccgaacaag cgtttactca actgatgtgg
     4021 cacgaatgat tcaagcaccg attttccatg ttaacggtga tgaccccgaa gcagttgtcc
     4081 gcgtagcaaa gttagctttt gacttcatgc aggctttcca caaagacgta gttattgaca
     4141 tggtctgtta ccgtcgtcgt ggacataatg aagctgatga tccttcatta actcaaccac
     4201 taatgtacga aattattgac cagaagcgat cggttcgaaa gttatatacc gaagctctcg
     4261 ttggacgtgg tgacataacg gttgaggaag ctgaaggtgt ccttaagcac taccaagatg
     4321 agttagaaaa gattttccag gcgaccaagg ccgaaaatgc tgaaggacag ttctcttctc
     4381 attcagcaga agttgtgcaa accgggcaac aaactctcaa accacaaact ccaacaagtg
     4441 ccgttgatac gtcaatcagt cgcgaagtaa tcgatcgagt tgttgcttca cagctgaata
     4501 tgcctgaagg ctttaccatt catccacgtc ttgcaccaca attacaacgg cgaaaagaaa
     4561 tggtcgacac cgatgccatc gattggggta tgggtgaggc tctagcaatc ggctctttgt
     4621 taaccgaagg aagatcggtt cgcttagccg gtcaggattc acgtcgtgga acttttggtc
     4681 atcgccatgc ggtaattgtt gacaaaaaaa cgggctggca attcaagcca ctaaagcaat
     4741 gctatgaagg aaatgccaag ctttgggttt acgattcgct acttagcgaa tatgcagcga
     4801 tggggtttga gtacggttat tcagtaattc gacccgatgt attaacaatg tgggaagcgc
     4861 aatttggtga cttcgctaat ggcgcccaga caattattga cgaatttatt gctgctggtg
     4921 aacaaaagtg ggcgcaacgt agtcgagtca cgctattgct tccacacggc tacgaaggtc
     4981 aaggacctga ccactcttcg gctcgaattg aaagattctt gcaattgtgt gcgcaggaca
     5041 acataactgt ggcaatgcca actacgccag catccttctt ccacttactt cgctggcaag
     5101 tgcattcacc tcttgatcga ccacttgtaa tttttacgcc gaaatctcta ctgcgcgcca
     5161 aatacgcaac cagcaaagta agtgacttta cttctggaac attcacttcg attatcggtg
     5221 ataaagaggc gaactcagca gaagttaaaa ctgccctgct ttgcagtggc aaggtttact
     5281 acgatttggt ggcacatcgg gaaaaaactg gtcgcaaaga tgtcgcgatt attcgccttg
     5341 aacgtttata cccacttccc gcaattacgt tgccaccaga gttggcccga tttgaaaatc
     5401 tacaaacagt caagtgggtg caagaagagc cagctaacca aggtgcttgg tctttcatgg
     5461 cgatgaattt gccagacgtg attggtcgcc aacttgttgg tgtctctagg ccagcttcat
     5521 cttcaccggc agttggttcg caccaccgtc acgaaatcga acaagagaca ctagttaatc
     5581 aagctttcag ctaggaaaat aatgtacttc acagaccgcg gcatcgaaga acttgaaaat
     5641 cgtagaggcg aagaagaaat ttctattgct tggttggctg ctcgacttca ggaatttgtc
     5701 gatttgaatc ctgattttga aattccaact gaacggtttg ctacttggct ggccagactt
     5761 gatgacgaag tagaagagta atgctatcga tagtcgcaac tcatggcgac gccgaaaatc
     5821 cacttgccgc actctctgta actgacttac ctgaacctga accaattgat aactgggtaa
     5881 aagttgaaat taaagcggcc gcactaaacc atcacgatat atggacttta cgtggccagg
     5941 ccacagtaag tgaaaattta ccgatagttc ttggttctga tggtgccggt ataactgacc
     6001 aaggtatgcc agttattttg catgcggtta ttggtgatcc agtcaatggt gatgaaacat
     6061 tagatccaaa aagatcgcta ctcagcgaaa aatataatgg gacttttgca aaattcactt
     6121 gcgttccgaa gtacaactta atcccaaaac cagaatggct tacttttgag caagcagcgt
     6181 gccttcccac agcctggttg actgcttatc gaatgctctt tagtaaatcg ggcttagcaa
     6241 aagatgacac tgttttgatc cagggtgcat ctggtggcgt agcttcagct ttaattcccc
     6301 tggctaatgc agccggattt cgagtctggg taactacacg tacagaaagc aaaaaagaat
     6361 acgcactaaa actaggtgcg caccagattt tcgacaccaa tgcaaagttg ccagaaaaag
     6421 ttgatgcagt gatggagaca gttggtcaag caacctgggc acattcaatg agaagtttga
     6481 agccaggcgg ccgcattgtt gtatcgggtg ctacaactgg ggctaatccc ccagctgatt
     6541 taaaccggct cttcttcctg caattgcaga ttattggttc aactatgggc tcgaaagcag
     6601 aactacttga attgatttca tttctagaaa aaaccggact gcgtccagac attcacaaaa
     6661 tttatgactt cactcatgca aagaaggctt ttgaagactt aatcaatggt gatgtgcaag
     6721 gaaaattagt tttaacgccg agctaaccaa ttaggcaatc gatagatgac cttggcaata
     6781 atctgagttc tttttacttg gccatactgg cgtgagtcaa cgctaaccgc ctcgttatcc
     6841 ccaataattg ttattcggtc gttttcgatt tttgcgatgc gcttaatatc aattcgctct
     6901 ttgaaatcaa ctagtactac atcgccctct tgaaacgctg atccatattt aacaattact
     6961 cgttcacccg gagcataggt tggcaccatt gaaagaccct caatgattgc gattccaagt
     7021 gccatagctc aagtctctca ccagaagtaa catgaaatta cctgcagttc atggactgca
     7081 ggtgcacacc aaactgacct accgtaacct tagataagcg ttaaatttat ttttattttt
     7141 ggaggaacaa tgaaattctt tgccccaaca gttcgcgttt tcgcacactg cgacttacct
     7201 tgtggtgttt atgatccagc tcaggctcga attgaagcgc aatcagtaaa ggcatgtatg
     7261 gagaagtacc atgcatcaga tgatcctaat ttcaaagccc gtgcagtcgc tataaaagaa
     7321 gaacgctcac acatggttaa agatcatcta tgggttcttt ggaccgacta cttcaaggca
     7381 ccacactttg aaaaataccc tcagttacac cagctgttta atgatgcaac gaaattagca
     7441 ggtgctgccg gtactaaggg aactgttgat gttgcagtag cagatcaact gctttcaaag
     7501 attgatgaaa tcgctaatat cttcgctgaa actaaaaaag cctaagtatt tttagattca
     7561 ttagctaggc agagatcatt ggcatttcaa gtacgtgcgg ctacaccgcc agatgtggct
     7621 cagatgcatt cgatgattat cgaattagct gagtttgaaa aagcagtaga cgaagttatt
     7681 gccaccgaag agcaactcga acgagcgctc ttcggtggca cgtctcattc tgcacattca
     7741 gcgaataccc cacatggctc gcccgcactt tttgctcatg taattgagga tcccgaacag
     7801 ccgggtcaac ttgctggaat ggctatttgg tttctgaatt acagcacctg gcaaggtgag
     7861 cacggaattt atttagaaga tctttatgtt cggccaaaat atcgaggcaa aggtttaggt
     7921 aaagcattac taaaaaaact tgcccaagtt tgtaatgaaa atggatacac cagattccaa
     7981 tggtgggtac ttacctggaa tcagagtgcg attgatgttt ataaagggat tggcgccgtg
     8041 ccaatggatg aatgggtggt ctatcgtctg actggcgata agttaaccga attcagcaat
     8101 tagttttcaa gtggtgtttt taccaaagca taaattgcca ctgctgaact taaaccgact
     8161 agtgccccgg taattcgata attcaattca tcgccagcta aaagtgtgta ggccacgatc
     8221 agaccaaaca tttgagttac taaatacgga gttctagccc aattctggac tcgggcaaag
     8281 ccccgactca caaaaaagat ggcagcggca aaaataaggt aaataattga ttcaacaact
     8341 ggtgagccca gtgtcgactc cagccgcaca ccaataataa aaattgcaat tgcatatccg
     8401 ccaagtaaca aactctggct aagtccgatt aagtaactaa tccttcgcat actcaaacca
     8461 tatcgcgttt aggctgtagc tatgcgttcc ctactggttg taaattcgtt tgcaacaacg
     8521 actacgcccg aggtgcaagc cgttataact tcagctctct caaaccacct agatttaaca
     8581 gttattaaca ctgaagataa gtctcatgga attgaaattg gcaaaaatgc ggttaaagaa
     8641 ggtttcgaac tggtaatcgc actaggtgga gacggaacta taaatgaatt agcaaatggc
     8701 atgctagcaa aaacggaatc agccgaaatt ccattgcttg caggtttacc tggtggaaat
     8761 gcaaatgtgt ttattagaaa tttggggtat tcaaacgatc cggtgattgc tacagctcag
     8821 ttgatcgaga aaattaccca aaaatcttca agaaaaatcg gacttggcaa aatagatttt
     8881 gctggaaact ctcgatggtt cttattcaat gctggcttag gccttgatgc tcgagtccta
     8941 tctcgaatgg atgataagag aaatcaaggc aagctagcca gtgacttttc atacgcttta
     9001 atcacattaa aagaactttt caccgaagtt cgaaacggta aagcccaatt aacaatcacg
     9061 gaccaagatg gcaacactta ctccgaaacg cagtttgctt taatgattaa cttttcccct
     9121 tggacttaca taggcaaatt tgcagtttca ccgactaaaa aagtaatgga taccacttcg
     9181 ctcgacttat ttgcgccata tgagctttca gtttcaagaa cggcgcaact tgttagggac
     9241 cttgttttgc ggagtaactt aactaacaat gaaaatgttt tagtgttaga aaaacaaaaa
     9301 acacttaaaa tctctgcttc ggtacccact tggattcaga cggatggtga agctttagcg
     9361 ttagttactg cggtaagtgt gaaacatttc gccgactgct taacggttat tgcttaactg
     9421 ccacaaatta actattgacc aaactcccta agccaattgg ttttcgcctt gagaattttg
     9481 aagatttgga cgagtgagac gtaag
//
LOCUS       DBNHVN010000143        10107 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1884239, whole genome shotgun sequence.
ACCESSION   DBNHVN010000143 DBNHVN010000000
VERSION     DBNHVN010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 10107)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10107)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10107
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1884239"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /gene="tal"
                     /locus_tag="AC16Y1_02650"
     CDS             <1..1078
                     /gene="tal"
                     /locus_tag="AC16Y1_02650"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HIR5831395.1"
                     /translation="AAGVSVWLDDLDRHRIESGNLAELIETKSVVGVTTNPSIFEKAL
                     STGVSEYQEQLAQLSNKPVDQAVRELTVADVTAACKVFSGVYQKSNGIDGRVSLEVDP
                     RLANDTAGTANQAVELWQAVNQPNLMIKIPATDAGLPAITATLANGISVNVTLIFSVK
                     RYQQVIDAWLAGLEQAKASGKNLSEIHSVASFFVSRVDTLIDAKLVALGTPQALAMKG
                     KAGVANARLAWQEYENLLVSDRWLSLREAGANAQRPLWASTGVKDPAYPDTLYVTELV
                     APGCVNTMPEGTLNAVADHGLVTGDTVTGTYAEAHGVFSELSSLGISFDEVFATLEKE
                     GVDKFIAAWEQLLSGLTKVLNGAK"
     gene            1075..2592
                     /gene="zwf"
                     /locus_tag="AC16Y1_02655"
     CDS             1075..2592
                     /gene="zwf"
                     /locus_tag="AC16Y1_02655"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIR5831396.1"
                     /translation="MINPLRDPDDRRLPRVAGPCSLILFGVTGDLARKKIMPAIYDLA
                     NRGLLPPGFALVGFARREWADQDFAKVVYEAVKEHARTSFRDDVWQQLSAGIRFVAGD
                     LTSDESFDELKRVVEELDQTRGTLGNHAFYLSIPPNLFPSVIEQLKRSGLAQSQTDAW
                     RRVVVEKPFGHDLTSARELNKLVGSAFPSGSVFRIDHYLGKETVQNMLAIRFANTMYE
                     PIWNANYVDHVQITMAEDIGIAGRAGYYDGIGAARDVIQNHLLQLVALTAMEEPTSFQ
                     ADDVRIEKEKVLNAIVVPKDIAAYTARGQYAPGWQGGIEVKGYLEEDGVNPDSKTETF
                     AAIRLDIDNRRWAGVPFYIRTGKRLGRRVTEVAVIFKHAPHLPFSESATQELSNNALV
                     FRIQPDEGLTVRFGSKVPGTSLEVRDVNMDFAYGESFTEDSPEAYERLILDVLLGDPP
                     LFPRHEEVESQWKILDPILEFWARDGKPQPYVSGGWGPQSAYDLMARDGRTWRRP"
     gene            2589..3512
                     /locus_tag="AC16Y1_02660"
     CDS             2589..3512
                     /locus_tag="AC16Y1_02660"
                     /inference="COORDINATES: protein motif:HMM:NF042449.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HIR5831397.1"
                     /translation="MINLENTNANAVASAISAERRRMGSSTVGMVLNLLVLTDELNQS
                     DALRAASGAAREHPMRIIGLIERPGRGEAHLDAQIQVGGDDGPGELIACRLNGQLAKH
                     AGSVAIPLLLPDTPVVAWWPDGGPDEPATDPIGKHAARRITDTSEVGNYLEKLAIRLA
                     NYQPGDTDLAWTRATSWRTMLAAAFDQNVGTAKKVEIQVASRNPSGLLLAGWLVKRLN
                     VPTKVTHSKGPGITKVTIETDLGEVVLSRPDGHTATLKVPGTPASTVALGRRNLAELL
                     AEELRRLDSDEIYYQALTGVPLVELPDQGKK"
     gene            3516..4208
                     /gene="pgl"
                     /locus_tag="AC16Y1_02665"
     CDS             3516..4208
                     /gene="pgl"
                     /locus_tag="AC16Y1_02665"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HIR5831398.1"
                     /translation="MHQLEVISIADESALIDRIISDLTNEIDSLLTKQDTVHISLTGG
                     RIGSQISVLLLNSPIAANTKVNFWWSDERFLMANDEQRNDLVIPDNLRSAQNIHRMPT
                     GNDLVLETAVAQVQENLANFGQNQLMDICLLSIGPDGHVASLFPNHAALSTSELVVGI
                     SDSPKPPKLRITWSLNAINRSNQVWLIANGAEKSSAVTLVMAGAKDIPASNVHGLTKT
                     VLYAEKSALLPK"
     gene            complement(4232..4564)
                     /locus_tag="AC16Y1_02670"
     CDS             complement(4232..4564)
                     /locus_tag="AC16Y1_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016578660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HIR5831399.1"
                     /translation="MASGSAIRGSRVGAGPMGEAERGDAAPRIHVPYYCADGHETAPS
                     FAHDADVPDEWDCPKCGLPAGKDKENPPVQLKTEPYKTHLAYVKERREAKDGKAILDE
                     AIAKLRGK"
     gene            complement(4574..4807)
                     /gene="secG"
                     /locus_tag="AC16Y1_02675"
     CDS             complement(4574..4807)
                     /gene="secG"
                     /locus_tag="AC16Y1_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR5831400.1"
                     /translation="MIGALQVVLALTSLLLIILVLLHKGKGGGLSDLFGGGMSSSLGG
                     SSVAERNLDRITIAIALIWVTAVVALGLTVKVS"
     gene            complement(4854..5630)
                     /gene="tpiA"
                     /locus_tag="AC16Y1_02680"
     CDS             complement(4854..5630)
                     /gene="tpiA"
                     /locus_tag="AC16Y1_02680"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019433817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR5831401.1"
                     /translation="MSRMPLMAGNWKMNLNHIEAIALVQKLAYTLNDEDFTSLDVAVI
                     PPFTDIRSVQTLVDGDKLKLLYGAQDISAHEKGAYTGEVSAAMLAKLGCTFAIVGHSE
                     RRQHHGETDQVVNAKAKAAIRNEIIPIICVGEGLDTRKAGDQVKYTLAQVDGALAGLS
                     GTQVENLVIAYEPVWAIGTGEVATPDDAQEVCGAIRKRLSELHGNSADKVRILYGGSM
                     KADNVAALMAMPDVDGGLVGGASLDPDEFVSVCRYRMHGN"
     gene            complement(5631..6827)
                     /locus_tag="AC16Y1_02685"
     CDS             complement(5631..6827)
                     /locus_tag="AC16Y1_02685"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR5831402.1"
                     /translation="MTIRGIDELAVENKTVIVRSDLNVPLDGKTITDDGRIRASVPTI
                     KALSSRGAKVIVLAHLGRPKGEPDDKYSLAPAAQRLGQLLGQHVELVNEVSGKLAITA
                     VANAAAGDVLMLENVRFDPRETSKTASERESLASEWAALADFYVSDGFGVVHREQASV
                     TELAKALPHAAGNLVEAEAKVFSKVLTNPDRPYVVVLGGSKVSDKLGVIENLIKSVDR
                     LIVGGGMCFTFLAANGHNVGASLLEEDQIDVVKDLVTRAKQNGVEIVIPIDVVVADKF
                     AADANHKVVDVAQIPEGWMGLDIGPKTVQQFSAALEDAKTVVWNGPMGVFEMPAFANG
                     TKAIATAITKVKGTTVVGGGDSAAAVRLLGIPESGFTHISTGGGASLEYLEGKTLPGL
                     AVLEDN"
     gene            complement(6835..7839)
                     /gene="gap"
                     /locus_tag="AC16Y1_02690"
     CDS             complement(6835..7839)
                     /gene="gap"
                     /locus_tag="AC16Y1_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013287466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR5831403.1"
                     /translation="MSIRVGINGFGRIGRNFFRAIAASGADIEVVGVNDLTDNKTLAH
                     LLKYDSILGRLGQEVSYTDDSIIVGGKKIAALAERDPASLPWGKLGADIVIESTGHFT
                     DATKAAAHLAGGAKKVIISAPASNEDVTIVMGVNHQQYDSAKHNVISNASCTTNCLAP
                     MAKAIHDAFGIDHGLMTTIHAYTNDQVILDFPHKDLRRARAAATNMIPTTTGAAKAIG
                     LVLPELKGKLDGYAMRVPVPTGSATDLTAVLKKAATKEEINAVVKAAADGPLKGILSY
                     TEDPIVSSDIVTDPSSCIFDAGLTNVIGNTVKVVGWYDNEWGYSNRLVDLTKYVGSQL
                     "
     gene            complement(7886..8866)
                     /gene="whiA"
                     /locus_tag="AC16Y1_02695"
     CDS             complement(7886..8866)
                     /gene="whiA"
                     /locus_tag="AC16Y1_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018382720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HIR5831404.1"
                     /translation="MAMTSAVKDELSRLKVTKACCRKAEVSALLRFAGGLHIVGGKIV
                     VEAELDTGQAARRLRKDLMEIFGHDSEVSVVSGAGIRKGSRYVVRVTNEGEYLAKQVG
                     LVDQSGRPVRGLPPHIVSASLCDAEAAWRGAFLAHGSLTEPGRSSSLEITCPGPEAAL
                     ALVGSARRLGITAKAREVRGVDRVVIRDGDAISALLTRIGAHESVLAWEERRLRREVR
                     ATANRLANFDDANLRRSARAAVAAGARAARAIEILGDEIPAHLLEAGQLRVTHQQASL
                     EELGALADPPMTKDAIAGRIRRLLAMADKRAADLGIPNTEAGLTPDMLEG"
     gene            complement(8883..9821)
                     /locus_tag="AC16Y1_02700"
     CDS             complement(8883..9821)
                     /locus_tag="AC16Y1_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HIR5831405.1"
                     /translation="MRFFTKTKNVVALGGGHGLSSTLRALTQTNCNITAVVGVSDDGG
                     SSGRLRKEFGIVPPGDLRMALAALCEDEKWGSTWANVMQHRFSSKGELSGHAVGNLLI
                     TALWEETGDIVEGLDWVAKLLGAKGRVLPLCLEPLRIQAKVKTKGGLTKKVVGQVKVA
                     TTKDKIISIAIDPPNPKVCQQAVTAVLSADVIILGPGSWFTSVLNHFEVPEMKQALVT
                     TKAKRILVLNLQPQNGETSNFNPESYLESLAKANPGFTLDTVIADPRHVKNQKKLTTV
                     AAKLGAKVEMAEVGNNQAPMEQHNPDRLAYTFEQVF"
     gene            complement(9828..>10107)
                     /locus_tag="AC16Y1_02705"
     CDS             complement(9828..>10107)
                     /locus_tag="AC16Y1_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016325959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RapZ C-terminal domain-containing protein"
                     /protein_id="HIR5831406.1"
                     /translation="WDPELKELTGLDASVNDYVIEQPEAKAFLDNYANLVSLVEDGYL
                     REGKRFVTIAMGCTGGKHRSVAMAENLAARLVKNGVEVRVVHRDLGRE"
BASE COUNT         2684 a         2395 c         2245 g         2783 t
ORIGIN      
        1 tgccgctggt gtttcggttt ggttagatga tttagatcgg catcgaatcg agtctggaaa
       61 cttagccgaa ctaatcgaaa caaagtcggt agttggcgta actacgaatc cgagtatttt
      121 tgaaaaagca ctttcaactg gagtttccga gtatcaagag caattggcgc agttgagtaa
      181 taagccggtt gatcaggcag tacgggaatt aacggtcgcc gacgttactg ccgcttgcaa
      241 agttttttcc ggggtttatc aaaaatcaaa tggaattgat ggcagagttt cccttgaagt
      301 tgatccaaga cttgctaatg acactgctgg cacggcaaat caagctgtcg aattatggca
      361 ggcggtaaac caaccaaacc taatgattaa aattccagcc acggatgctg gcttacctgc
      421 gataactgcc acattggcta acggaattag cgttaacgtg acattgattt tttcagttaa
      481 gcgctaccag caagtaatcg atgcctggct agctggatta gagcaagcga aggcaagcgg
      541 aaaaaacttg agtgaaattc attcggttgc ttcgttcttt gtttcgcgcg ttgatacttt
      601 gatagatgcg aaattggttg cactaggcac gccacaggct ctagcgatga aaggtaaagc
      661 tggggtggca aatgcccgtc ttgcttggca agaatacgag aatttgttag taagcgatcg
      721 atggctatcg ctgcgcgaag ccggcgccaa cgcgcagcga ccactctggg cttcgaccgg
      781 agttaaggat ccggcctacc cagatacttt gtatgtaact gaacttgttg ccccaggttg
      841 tgttaacaca atgccagaag gaactttgaa tgcagttgca gatcatggct tagtcacggg
      901 tgacacagtt actggtactt acgcggaagc tcacggagta ttttccgaac tttcatcact
      961 aggaattagt ttcgatgaag ttttcgccac tttagaaaaa gaaggtgtcg ataaattcat
     1021 tgctgcttgg gaacaactac ttagtggcct cactaaagtg ctaaacggag ctaagtgata
     1081 aatccgcttc gtgatcctga tgatcggcga ctacctagag ttgctggccc ctgtagttta
     1141 attttgtttg gtgttacggg tgatttagct cgaaaaaaaa taatgccagc aatttatgac
     1201 ttagcaaatc gcggtctatt gccaccaggt tttgcactag ttggttttgc tcgacgtgaa
     1261 tgggctgatc aagattttgc caaagtagtt tatgaagccg ttaaggaaca tgctcgaact
     1321 tcatttcgag atgatgtctg gcagcaactt tcggctggta ttcggtttgt ggccggcgac
     1381 ttaacaagtg atgagtcatt tgatgaatta aagcgtgtag ttgaagagct agaccaaact
     1441 cgtggcacat taggtaacca tgcgttttac ctttcaattc cgcccaactt attcccttcc
     1501 gttatcgaac aacttaaaag gtctggccta gcccaaagcc aaactgacgc ttggcgtcgc
     1561 gtggttgttg aaaaaccttt tggtcatgac ttaactagcg ctcgcgaatt aaacaagttg
     1621 gtaggcagtg catttcctag tggcagcgta tttcgcatcg accattactt aggtaaagaa
     1681 actgtgcaaa atatgttggc cattcgcttt gcaaacacga tgtacgaacc gatttggaat
     1741 gctaattatg tcgaccatgt gcagattacg atggccgagg atattggtat tgctggtcga
     1801 gctggttact acgacggtat cggtgcggct agagacgtca tccaaaacca cttgttacaa
     1861 cttgtagctt tgaccgcaat ggaagaaccg acttcatttc aagccgatga cgtgcgaatt
     1921 gaaaaggaaa aagtacttaa tgcaatcgtg gttccaaaag atattgctgc ttacaccgcg
     1981 cgaggtcaat acgcaccggg ctggcaaggt ggaatcgaag ttaagggtta tctagaagaa
     2041 gatggcgtca atccagattc aaaaaccgag acttttgcgg ctattcgact agacattgat
     2101 aaccgtcggt gggctggtgt tccgttttat attcggaccg gaaaacgact tggtcgacga
     2161 gtgaccgaag ttgcggtgat ttttaagcat gccccgcact taccttttag cgagagtgcc
     2221 acccaagaac taagcaacaa tgcgttggta tttcggattc agcccgatga aggtttgacc
     2281 gtacgctttg gctcaaaagt acctggtact tcgcttgaag ttcgagacgt aaacatggat
     2341 tttgcatatg gtgaatcatt tactgaggac agcccagagg cttacgagcg acttattctg
     2401 gacgtgctac tcggtgatcc accgctattc ccaagacatg aagaagttga atcacaatgg
     2461 aagatcttgg acccaattct tgagttctgg gcaagagacg gtaaacctca gccttatgtt
     2521 tcaggtggct ggggacccca gagtgcttat gacttaatgg cccgcgacgg cagaacttgg
     2581 cgacgcccat gattaatttg gaaaacacca atgcaaatgc ggtcgctagt gcaatcagcg
     2641 cagaacgccg gcggatgggt agttccacag ttggcatggt gttaaatttg ctggttttaa
     2701 ctgatgagtt aaatcaatcg gatgctctgc gtgctgcaag tggcgctgct cgtgaacatc
     2761 caatgcgaat tattgggtta atcgaacgcc ctggccgcgg tgaggcacat cttgatgccc
     2821 aaatacaagt tggtggcgac gatggtcctg gcgaattaat tgcttgccga ctaaatggcc
     2881 agcttgctaa acatgctgga tctgtcgcaa tcccattact tttacccgat accccagttg
     2941 ttgcctggtg gcccgacggt ggcccagacg agccggccac cgatccaata ggtaaacatg
     3001 cggccaggcg aattaccgac acaagtgaag ttggtaatta cttagaaaag ttggcgatcc
     3061 ggttggccaa ctatcaaccc ggtgataccg atctagcttg gaccagagcg acttcttggc
     3121 gaactatgtt ggcggctgct tttgatcaaa atgttggaac cgcgaaaaag gttgaaatcc
     3181 aagttgccag tcgaaatcca tctggattat tgcttgctgg atggttggtt aaacgtttga
     3241 atgtgccaac aaaagtaacg cattcaaagg gaccaggaat tacaaaagtg actatcgaaa
     3301 ctgatctggg tgaagttgtc ctgagtcgac ctgatggaca tactgcaaca cttaaagttc
     3361 ctggtactcc tgcatcaact gttgcactgg gcaggcgcaa tttggccgaa ttacttgctg
     3421 aagaattacg gagattagat tcggacgaga tttactatca ggcgttaact ggggtgccgc
     3481 tagttgagtt accggatcaa ggaaaaaaat aacctatgca ccaacttgag gttatttcaa
     3541 tagcagatga atcagcattg attgatcgaa taatttctga tttaacaaat gagattgatt
     3601 cgctgctcac gaagcaagat accgttcaca tttcacttac tggtggccga attggtagcc
     3661 agatttcggt cttgttactc aattcgccta tcgcggcaaa tacaaaagtg aacttttggt
     3721 ggtcagatga gcgtttctta atggccaatg atgagcagcg taacgattta gttattccag
     3781 ataatttgcg ctctgcgcaa aatatccatc ggatgccaac tggaaatgac ttagttttag
     3841 aaactgccgt tgcacaagta caagagaacc tggcaaattt tggtcaaaat caactcatgg
     3901 atatttgttt attaagtatt ggtccagatg ggcatgtggc ttcactgttt ccaaatcacg
     3961 ctgccctatc aactagtgaa ttagtagttg gcattagtga cagccctaaa ccacctaagt
     4021 tgcgaatcac ttggtcactt aatgcaataa accgaagtaa tcaggtttgg cttattgcaa
     4081 atggtgcaga aaaatcatct gccgttactt tggttatggc cggtgccaaa gacatacctg
     4141 caagtaacgt acatgggtta actaaaaccg ttctatatgc agaaaaatcc gctctacttc
     4201 ctaagtaaga gcggatcttt cgtgaaatta attacttacc gcgtaacttg gcaatcgctt
     4261 catcgagaat ggctttgccg tctttagctt cgcgacgttc tttcacataa gctaagtgag
     4321 ttttgtaagg ttcggtctta agttgcactg gcggattttc tttatcctta cccgctggta
     4381 aaccgcactt tggacaatcc cattcgtctg gaacgtcagc atcgtgggcg aaacttggtg
     4441 cagtttcatg tccatcagca cagtagtaag gaacgtggat gcgcggggca gcatcgccac
     4501 gttctgcctc acccataggg ccagcaccaa cgcgactgcc tcgaatagca ctaccacttg
     4561 ccataggaac tccttaacta acttttacgg ttaaaccaag agcaactact gcagttaccc
     4621 aaatcaatgc aattgcgatc gtaatacgat ccaaatttcg ctcagcaaca gatgagccac
     4681 caagtgaact actcataccg ccaccaaaga gatcagaaag accgccacct ttacctttgt
     4741 gtaataacac caagatgatt aacaacagac tggtaagtgc cagtactact tgtagtgcgc
     4801 cgatcatgaa gatcccttct aacccttgaa aattttaccc gacctacgcc gaattaatta
     4861 ccatgcatcc gatagcgaca taccgagaca aattcgtctg gatcaaggga tgcaccgcca
     4921 actaatcccc cgtcaacatc tggcatagcc ataagggcag cgacattgtc agccttcatt
     4981 gagcccccat aaagaatgcg cactttgtct gcagaattac cgtgtaattc actaagtcgt
     5041 tttcgaatag cgccacatac ttcttgggcg tcatctggcg tagctacttc accggtacca
     5101 attgcccata ctggctcata ggcaataact aaattctcaa cttgtgttcc agataaccca
     5161 gcaagggcac catcaacttg ggccaaggtg tatttgactt gatcgccggc tttgcgagta
     5221 tctaatcctt caccaacaca aataattggg atgatttcat ttcggatcgc ggcttttgct
     5281 ttggcattca ctacttgatc agtttcacca tggtgttggc ggcgttcaga gtgaccaaca
     5341 atcgcaaaag tacaaccaag tttggctaac attgctgcac ttacttcacc ggtgtatgca
     5401 cctttttcat gtgcggagat atcttgcgca ccataaagta atttgagttt gtcaccatct
     5461 accaaggttt gtacggagcg aatatctgta aacggtggga tgactgccac gtcaagacta
     5521 gtgaagtctt cgtcgtttaa ggtgtaggca agtttttgaa ctaacgcaat cgcctcaata
     5581 tggttgaggt tcattttcca gttacctgcc attaacggca tacgtgacat ttagttatcc
     5641 tccaagacag ctaagccagg caatgttttg ccttctaaat actcaaggct ggcgccgcca
     5701 cccgttgaaa tatgcgtgaa acctgattct gggataccaa gtaacctaac agcggcagcg
     5761 ctgtcgccac caccaacaac tgtagttccc ttaacttttg tgatcgcagt ggcgattgcc
     5821 tttgtgccat tggcaaatgc cggcatttca aaaacaccca tcggaccatt ccaaactaca
     5881 gttttggcat cttcgagcgc ggcgctaaat tgctgcacag tttttggtcc aatgtccaaa
     5941 cccatccagc cttcagggat ttgcgcaaca tctactacct tgtggtttgc atcagctgca
     6001 aacttatctg caaccactac atctatagga attactattt ctacgccatt ttgctttgct
     6061 cgagtcacta aatctttaac tacatctatt tgatcttctt caagtaaaga tgccccgaca
     6121 ttatgaccat tggctgctaa aaatgtaaaa cacataccgc cgccaacgat taatcgatca
     6181 actgacttga tcaagttttc aataacaccg agtttgtcac taacttttga gcccccaaga
     6241 acaactacat atggtcgatc tgggttagta agtactttgc taaatacttt tgcttcagct
     6301 tcgactagat ttccagccgc atgtggaagt gcttttgcta attcagttac ggaggcttgc
     6361 tctcgatgaa ctaccccaaa tccgtcactg acataaaagt cagcaagtgc cgcccactca
     6421 cttgctagag attcgcgttc actggctgtt ttacttgttt cccggggatc aaagcgaaca
     6481 ttttctagca tcaagacatc accagctgca gcatttgcta ccgctgtaat tgctaatttg
     6541 ccactgactt catttactag ctcaacgtgc tggccaagta attgcccaag acgctgcgct
     6601 gccggtgcta aagagtattt atcgtcgggc tcacctttag gtctgcctaa atgcgccagc
     6661 acaatcactt ttgcgccacg actgctaagt gcttttatcg ttggcacact agcccggatc
     6721 cggccatcat cagtaatcgt cttaccatcc aatggaacat tcaaatcaga acgtactatt
     6781 actgttttat tctcaacagc taattcgtca atcccgcgaa tagtcataaa ttatttaaag
     6841 ttggctaccg acgtatttag ttaagtcaac taagcggttt gagtaacccc attcgttgtc
     6901 ataccaaccc acaactttaa ctgtgttgcc aattacgttt gttaaaccag catcgaaaat
     6961 acaagaagac ggatcggtaa ctatgtcgga agaaacgatt ggatcttcgg tgtagctcaa
     7021 aatacctttc aatggaccat cagccgctgc tttaaccaca gcatttattt cttctttagt
     7081 agctgctttt ttaagtactg cggttaggtc agtagcactg ccagttggaa ctggtacgcg
     7141 catcgcgtaa ccatctagct ttcctttgag ttcaggtagc actaaaccaa tggcttttgc
     7201 tgcacctgtt gtggttggaa tcatgtttgt tgcggcagcg cgggcacgac gtaaatcttt
     7261 atgtgggaaa tccaaaatta cttggtcatt tgtataagcg tgaattgtgg tcattaagcc
     7321 atgatcaatc ccgaacgcat cgtgaatagc tttggccata ggtgcaaggc agtttgtcgt
     7381 acatgatgca ttagaaatga cgttatgttt tgctgaatcg tattgctgat gattaacgcc
     7441 catcacaatc gtgacatctt catttgaagc tggcgcagag attatcactt tcttggcgcc
     7501 gccagctaaa tgggctgcgg cctttgtcgc atctgtgaaa tggccggttg attcgatcac
     7561 gatgtcggca ccaagtttgc cccatgggag tgaagcagga tcgcgttcag ctagtgccgc
     7621 gattttcttg ccaccaacga taatcgagtc atctgtataa gaaacttctt gacctagtcg
     7681 gcccaaaatt gaatcgtatt taagtaagtg cgccagagtt ttattgtcag tcaagtcatt
     7741 aacgccaact acctcaatgt cggcgcctga agctgctatc gcgcgaaaaa aattacggcc
     7801 gattcggcca aaaccattaa taccaactcg gatgctcatc aggatccttt caggatttat
     7861 aaataaatcc cgttaaaact cgctcttatc cctcgagcat atcaggggtc aaaccggctt
     7921 ccgtattagg gatgccgaga tcagcagctc ttttgtctgc cattgctaat agccgtcgaa
     7981 tacggccagc aattgcatcc ttagtcatcg gaggatctgc cagcgcacct agttcttcaa
     8041 gactggcctg ttgatgtgtt actcgtaatt gtcccgcttc aagaaggtgg gctgggattt
     8101 catcacccag aatttcaatt gcccgggcgg ctctggcccc agcagcaacc gctgcccttg
     8161 cggagcgacg taagttcgca tcatcaaaat tagctaagcg attcgcagta gctcgaactt
     8221 cacgccgtag gcggcgttct tcccaagcaa gtactgactc atgggcacca attcgagtta
     8281 ataacgcact aattgcatca ccgtcacgaa taactacccg atcaacaccg cgcacttcac
     8341 gtgcctttgc ggtaattcct agtctgcgtg cacttccgac taaagcaagt gccgcttcag
     8401 gaccaggaca agttatttca agtgatgaac tgcgacctgg ttcagttagc gaaccatgtg
     8461 ctaaaaatgc tccgcgccac gctgcctcgg catcgcacag tgaagcagag actatatgtg
     8521 gtggtaaacc tcgtactggt cgtccacttt gatcaactaa accaacttgt ttagctaaat
     8581 actcaccttc attagtaacc cgaactacgt aacgactgcc tttgcgaatt ccggcaccac
     8641 tgaccaccga gacttcactg tcatgaccaa aaatttccat taagtctttg cgtagacgtc
     8701 gtgccgcctg gccagtatct agttcagctt cgacaacaat ttttccgccg acaatgtgta
     8761 acccaccagc aaatcgcagt aaggctgaaa cttcagcttt gcggcaacat gctttagtca
     8821 ccttcaatct gctgagttcg tccttaaccg ctgaggtcat tgccatttac cgttcctacc
     8881 gcttaaaaaa cttgctcaaa ggtgtacgct agccgatcgg gattgtgctg ctccattggg
     8941 gcttggttat ttccaacttc agccatttcc actttggccc ccagttttgc cgctaccgtc
     9001 gttaatttct tttgattttt cacatgtctt gggtccgcaa tcacggtatc tagcgtgaaa
     9061 ccaggattag ctttagcaag tgactctagg taactttctg ggttgaagtt actggtctca
     9121 ccattttgcg gctgcaggtt tagcaccaga attcgcttag ctttagtggt cactaatgct
     9181 tgtttcattt ctggaacttc aaagtgattc agcacactgg taaaccaaga tcctggaccc
     9241 aaaataatta catctgcact tagcaccgct gtaaccgctt gttggcaaac ttttggattt
     9301 ggtgggtcaa tcgcgatcga aattatttta tctttagtgg tcgcaacttt tacttggcca
     9361 actacttttt tagtaaggcc acctttcgtt ttaacttttg cttggattcg aagtggctct
     9421 aaacaaagag gcaaaactct gccctttgcg ccaagtaatt tagccaccca atctaaacct
     9481 tcgacaatgt caccagtttc ttcccagagt gcagtaatca acaaatttcc aactgcatgg
     9541 ccggataatt caccctttga actaaatcgg tgctgcatca cgtttgccca agttgagccc
     9601 cacttttcat cttcacaaag tgcagctaat gccattcgta aatcaccagg tggcacaatg
     9661 ccaaattctt tgcgtaggcg tccactagag ccaccatcgt cactcacacc gacaactgca
     9721 gtgatgttgc agtttgtttg ggtaagggca cgaagagttg aagacaatcc atggccgcca
     9781 ccgagcgcga caacattctt tgtcttagta aaaaaacgca agattaatta ctcccgacca
     9841 aggtcgcgat gaactacccg aacttcaacg ccatttttaa ctaaccgcgc agcaagattt
     9901 tccgccattg ccacacttcg atgtttaccg ccagtacaac ccatggctat tgtgacgaaa
     9961 cgtttacctt cgcgtaagta accatcttca accagcgaaa ctaagtttgc gtaattatca
    10021 agaaaagctt tggcttctgg ttgttcgatt acatagtcat ttaccgaagc atctaatcca
    10081 gttaattcct ttaattctgg atcccaa
//
LOCUS       DBNHVN010000144         1248 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1290389, whole genome shotgun sequence.
ACCESSION   DBNHVN010000144 DBNHVN010000000
VERSION     DBNHVN010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1290389"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="AC16Y1_02710"
     CDS             <1..513
                     /locus_tag="AC16Y1_02710"
                     /inference="COORDINATES: protein motif:HMM:NF023917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3710 domain-containing protein"
                     /protein_id="HIR5831407.1"
                     /translation="GSVWVPSFLGLDISAEIAQQDRQILSLSLIYANSIAKVEVFAAA
                     KDHSSWSDTRFELAARLEETKVQPKVSSGSFGPELSAVMPTFDAQGNALVQAVRFLGI
                     DGDRWFMRVTVSGDAATEEDAIKKLDDLICQLVVNRGDEAMSPGTRLPLSFPTGQAVA
                     QSEENLRFEI"
     gene            530..895
                     /locus_tag="AC16Y1_02715"
     CDS             530..895
                     /locus_tag="AC16Y1_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HIR5831408.1"
                     /translation="MSRVIDYFKSLVESQIDLDSAVLRRQTELIGASAAARCVVGEFV
                     KVQGTIRALRNRSHDNFPVVEVELWDGTGYITLLWMGRAKIRGISVGRQLIVQGRISR
                     GPKQQPAIYNPKYELLPNE"
     gene            888..>1248
                     /locus_tag="AC16Y1_02720"
     CDS             888..>1248
                     /locus_tag="AC16Y1_02720"
                     /inference="COORDINATES: protein motif:HMM:NF022805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HIR5831409.1"
                     /translation="MSDRKESIASELADDAKLLTSSLGGARGMFESGAPAITFIAVYG
                     LTSQNLRASVFAAVSIGLVLAILRLVKRQTLSQVTAGFIGLAFSSWLAIASGRAENFF
                     LPGILTNFLYAGVCLISL"
BASE COUNT          336 a          246 c          311 g          355 t
ORIGIN      
        1 ggttcggttt gggtaccatc atttttgggt ttagacatct ctgcagaaat agcccagcaa
       61 gatcggcaga tcctttcgtt aagtctgatt tatgcaaatt caattgccaa agtagaagta
      121 tttgccgcag caaaggatca cagctcttgg tctgatacgc gatttgagct tgctgcaaga
      181 ttagaagaaa ccaaagtgca acccaaagta tcaagtggaa gttttggtcc cgagttaagt
      241 gccgtaatgc ctacttttga tgcacaaggt aacgcgcttg ttcaggctgt tcgatttctt
      301 ggtattgatg gcgacaggtg gttcatgcgg gtaactgtta gtggagatgc ggctactgaa
      361 gaagacgcaa ttaaaaagtt agacgatcta atttgccaat tagttgtaaa tcgaggcgat
      421 gaggcgatgt cgcctggaac tcggttaccg ctctcgtttc ctactggcca agcagttgcg
      481 cagagtgaag aaaacctgag atttgaaatt taaaaggtaa agtttaggca tgagccgagt
      541 tattgattac tttaaatctt tggtggagag ccaaattgat ttggattcgg cagtacttcg
      601 cagacagaca gaactgattg gcgccagcgc agcagccaga tgtgtagtcg gtgaattcgt
      661 aaaagttcag ggcactatca gagcccttag aaatcgctcg cacgacaatt ttcccgtagt
      721 tgaagttgaa ctgtgggacg gtactggcta catcacgtta ctttggatgg gaagagctaa
      781 aattcgaggt atctcagttg gccggcaact aattgtgcaa ggcagaattt cccgtggacc
      841 taaacaacaa ccggcgattt acaaccccaa gtatgaattg ctcccaaatg agtgaccgca
      901 aagaatccat cgctagtgaa cttgctgatg atgcaaaact tttaacctct tcactcggtg
      961 gcgctcgcgg aatgtttgaa agcggcgctc cagcgatcac atttatcgca gtatatggat
     1021 taacttcaca aaatcttcga gcaagtgtgt tcgctgcagt aagtattggt ttagttttag
     1081 caatacttcg attagttaag cgccaaacgc tatctcaagt gactgccgga tttatcgggt
     1141 tagctttttc atcgtggttg gcgatcgcaa gtggtcgagc ggagaacttt tttctacctg
     1201 ggattttaac taattttcta tatgcaggtg tttgcttaat cagtttga
//
LOCUS       DBNHVN010000145         1262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_28797, whole genome shotgun sequence.
ACCESSION   DBNHVN010000145 DBNHVN010000000
VERSION     DBNHVN010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1262
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_28797"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..631
                     /gene="nadD"
                     /locus_tag="AC16Y1_02725"
     CDS             65..631
                     /gene="nadD"
                     /locus_tag="AC16Y1_02725"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIR5831410.1"
                     /translation="MRVGLMGGTFDPIHIGHLVAASEVHSSLNLEQVIFIPAGNPWQK
                     SDLSLTPSVERLEMVKQAIAGDDRFTLSDIEGTRSGPTYTIDTIVELQKSNPNNEYFL
                     ILGTDALINLHTWHEFEKLTKLVTVVGVNRNGVNQIELDFDYIFVQIPEIAVSATDIR
                     NRVKTAKPIKYLVPSAVERLIEKLGLYK"
     gene            631..>1262
                     /locus_tag="AC16Y1_02730"
     CDS             631..>1262
                     /locus_tag="AC16Y1_02730"
                     /inference="COORDINATES: protein motif:HMM:NF015755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family glycopolymer transferase"
                     /protein_id="HIR5831411.1"
                     /translation="MNRVPEFIKWLTATLLSLALLTAIFFIRSQQIEEQAAFTPVAKS
                     EIKTVLLQLAEDDKSISGLALLIKESGSGLQVINISPQIATQFGQAGLMTVQEAGTQV
                     SPGDVADALGVATGIRIDATLALQRLAIAGLVDSVGGVDVSAQTGLLVSGLDQDPLYV
                     APGQQRLTGQYAAGYALVKQVVESEDEQINRMNNVIRAMFINLPVDQSTT"
BASE COUNT          396 a          239 c          276 g          351 t
ORIGIN      
        1 gatgaatatc ttggttaggc tgttagttag ttaaactttt taagagtatt aacgaggagt
       61 ttaagtgcga gttggcctaa tgggtggcac ttttgatccc attcacatcg gccacttagt
      121 tgcagcaagc gaagtccata gttcgttaaa tctagaacaa gtgattttca ttccagctgg
      181 aaatccttgg caaaagtctg atttatctct aacacctagt gttgaacgac tagaaatggt
      241 taagcaggct attgcaggtg atgaccggtt tacattaagt gacatagaag gaacgagatc
      301 tggtccaact tacacaatcg ataccattgt tgaacttcaa aaaagcaatc caaataatga
      361 gtatttttta atccttggca ctgatgcgct gataaacctg cacacctggc acgagttcga
      421 aaaattaaca aaattagtaa ctgttgttgg cgtgaatcga aatggtgtaa atcaaatcga
      481 attagatttt gattacattt ttgttcaaat tcctgagatc gctgtatcag ccacagacat
      541 taggaatcga gtaaaaacag caaagccaat taagtactta gtgccaagtg cagtagaacg
      601 gttaatcgaa aaactagggc tatataaata gtgaatcggg taccagaatt tattaagtgg
      661 ctaaccgcaa ctttactctc attggcttta cttacagcta tattttttat tcgtagtcaa
      721 caaatagaag agcaagccgc atttacccca gttgctaagt cagaaataaa aacagtttta
      781 ttgcaattag ctgaagatga taaatcaatt tccggcctag cacttttaat taaagagtct
      841 ggtagcggac ttcaggtgat caacatttcc cctcaaatag ctactcaatt tggtcaagca
      901 ggattgatga cagtacaaga agccggcacc caagtttcgc caggagatgt cgctgatgca
      961 ttgggtgtgg ccaccgggat tcgtatcgat gcaactttag ccttacaaag gctggcaatt
     1021 gccggcctag tcgacagtgt tggcggggtg gatgtatctg cgcaaacagg actattggta
     1081 agtggtctag atcaagaccc gctttatgtt gcgccaggac agcaaagatt aactggacaa
     1141 tatgccgccg gatatgcatt ggtcaagcaa gttgttgaaa gtgaagatga gcaaataaac
     1201 cgaatgaata acgtcattcg agcaatgttc attaacttac ctgtcgatca gtcaaccacc
     1261 ga
//
LOCUS       DBNHVN010000146         3821 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1810650, whole genome shotgun sequence.
ACCESSION   DBNHVN010000146 DBNHVN010000000
VERSION     DBNHVN010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1810650"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="AC16Y1_02735"
     CDS             <1..852
                     /locus_tag="AC16Y1_02735"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5831412.1"
                     /translation="DAVGPSRLRTAYAIDTTMMNATTVVGPVLTTYLALRFSGEHALW
                     MTSILMGIGGTAMMTMPLSRNWKPETVKPTTKGLFKNRAFRIIVFEGMIFGFGWGLME
                     ISIPAYSSLIERPELSAPLLATLAITSIVGGLIIGGRKSSITPLNGFKVAGLAVAICA
                     APLAFTSPGMSMGIVLAALGLALGFAQVYHWETLEAIRPAGTATSAQAWLWTLEGSML
                     AIGAAFGGFLVEKVNPQVALGLISACLLGATSYIWLYGSKHLQGANKPLSEVKKVEAL
                     ADLETPT"
     gene            955..1212
                     /locus_tag="AC16Y1_02740"
     CDS             955..1212
                     /locus_tag="AC16Y1_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024835.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5831413.1"
                     /translation="MAKSAPRGKSKTGLDWLRDRMDTLGYNSLESVAQEIGINRGNLY
                     RYFSLETRPSVALLPDLCRALKSSPAEILKALEILRADDRL"
     gene            complement(1189..1470)
                     /locus_tag="AC16Y1_02745"
     CDS             complement(1189..1470)
                     /locus_tag="AC16Y1_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831414.1"
                     /translation="MIELVLSEQISDPVVERLTGPRVRVSINPEQSLDSAINQCRPDL
                     HQAIEWALTTLYENDEAPHRVYVRDANGQSMVAMTDPCINYGSQAIIRP"
     gene            1658..2965
                     /locus_tag="AC16Y1_02750"
     CDS             1658..2965
                     /locus_tag="AC16Y1_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013715.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HIR5831415.1"
                     /translation="MEKPEGEPLARAFLGIAKEEQHHPIRTQISSAGFFNRLGPGIVT
                     GAADDDPSGIGTYSQIGAVQGYRALWVAPVLLPFAFAIQETCARIALVTGEGLARVIK
                     RKFPAPILFLMITLVAIANTVNIAADLSSMAAVIRMFIPLNQFLVVIMITAVVLVAEV
                     LIPYHKYARVLRWLCLSLLSYVAVLFVADIDWAAVGAGLTSISISWSKSDIAALIAVA
                     GTTISPYLFFWQAAEEMEEGLQAPDISVTHIHAMRGDVFAGMLSGVFVMFAIMATTAA
                     TLFGTGITSITSPEQAAAALKPVAGEFASVLFALGILGTGLLAVPVLAGSTAYAVSEA
                     FGWKESLERKPSEAKQFYAVLAASMLIALLLNLIGVNPMQSLILAAIINGITAPILML
                     VIWKISRDQTLLGQWKSPMWSSTLVGIGTIGMFALPICWLFAN"
     gene            complement(2951..3529)
                     /locus_tag="AC16Y1_02755"
     CDS             complement(2951..3529)
                     /locus_tag="AC16Y1_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIR5831416.1"
                     /translation="MSSVSASAVLADRWVNRSIAKSVLLVFGLTGVTAASAQIAIPLP
                     FTPVPLTLQTFAVLVGAASLGATRSVLAQTLYFALAAAGAPVLAGGAGGIDKALGATG
                     GYLIGFIVASFVVGSIASRGATTQIRSTVLAYIAGSAVIYLLGFIWLAQFTGNGFAWA
                     LTHGVTPFIVGDIIKAAAAGLLLPSAWKLVSK"
BASE COUNT         1017 a          870 c          861 g         1073 t
ORIGIN      
        1 gatgctgttg gaccaagtcg acttcgaact gcatatgcaa tcgacaccac aatgatgaat
       61 gccaccacag ttgtgggtcc tgttttaaca acttatctag ctttgcgatt tagcggtgag
      121 catgcccttt ggatgacatc aattctgatg ggtattggtg gtactgccat gatgacaatg
      181 ccgttatctc gaaattggaa acctgaaaca gtcaagccaa ccacaaaggg tttatttaaa
      241 aaccgagcct ttcgaataat agtttttgaa ggaatgattt ttggatttgg ttggggttta
      301 atggaaatct ccatccctgc ctactcgtcg ctaattgagc gacctgagtt gtctgctcca
      361 ttacttgcaa cgctggcaat aaccagcatc gtcggcggct taattattgg cggccgtaaa
      421 tcttcaatta ccccacttaa cggttttaaa gttgcaggtt tagcagtggc aatatgtgcc
      481 gcgccacttg cattcacttc tcccggaatg agtatgggta ttgtgctggc tgcactcggt
      541 ttggcccttg gctttgcgca ggtttaccat tgggaaacac tcgaagcgat cagaccagct
      601 ggaactgcaa cttccgctca agcctggcta tggacattag aaggttccat gttggcaatc
      661 ggggcggctt ttggtggttt cttggttgaa aaagttaacc cccaggttgc tcttggactt
      721 atttctgcat gtctattggg cgcaacttca tacatttggc tctatggcag caagcatctt
      781 caaggggcaa ataaaccact ttctgaagtt aaaaaagttg aggccttagc tgacttggaa
      841 actcctacct aaatttcgcc ccgactttgc gtgctcaatt ggaaagttat aatttttaga
      901 gtaataatga attcaatttc ataataatga actatccatt tagaaaagga aaaaatggct
      961 aaatcggcac ctaggggcaa gtcaaaaact ggcctcgatt ggttgcgcga tcgaatggat
     1021 accttgggtt acaactcact tgaatctgta gctcaagaaa ttggaatcaa ccgcggaaac
     1081 ttgtatcgat acttttcgtt agaaacaaga ccgagtgttg ctttacttcc agacttatgt
     1141 cgcgcattga aatcttcccc tgcggaaatc ctgaaagcgc ttgaaatatt aagggcggat
     1201 gatcgcttgt gatccatagt tgatgcacgg gtcagtcatt gcaaccattg attgaccatt
     1261 tgcatcacga acatacactc gatggggcgc ttcatcattt tcatacaaag tggtgagcgc
     1321 ccattcgatt gcctggtgta aatctggccg gcactgatta attgctgaat ccaaactttg
     1381 ttccggattt atcgatactc gaactcgcgg cccagttaat ctttcaacta ctggatcact
     1441 tatctgttca ctgagcacta gctcaatcat taaattcctt tacctcagaa caccattcgc
     1501 atttgaggac cattgttgtg attaaacatc atactgttag atctgctcaa attcatggtt
     1561 catcgctgcc ctaattcact caaacatcgg tccctgacaa aaattttcct tattaataag
     1621 gctcgaataa cagtcatgta gttcgttaga gttaagtgtg gaaaaacccg aaggcgagcc
     1681 attagccagg gcttttttag gtattgctaa agaagagcag catcatccaa tccggaccca
     1741 aatttctagt gctggtttct tcaatcgatt aggccccggt atagtcacgg gtgctgcaga
     1801 tgatgatcca tcaggtatcg gcacatactc tcaaattggt gcagtccagg gctatcgagc
     1861 actctgggtt gccccagtgt tactcccatt cgcttttgca atccaagaaa cttgcgcccg
     1921 gattgcctta gtaaccggtg agggtttggc ccgagtaatc aaacgtaaat ttccggcacc
     1981 aatccttttt ttaatgatta ctttggttgc aattgcaaac acggtgaata ttgctgccga
     2041 cttaagttcg atggccgcgg taattcgcat gtttatcccg ctcaatcagt tcttagttgt
     2101 aattatgatc actgctgtag tcctcgtggc cgaagtccta atcccctatc acaaatacgc
     2161 tcgagtgctt cgctggcttt gcctttcgct actaagttac gtagcggttt tatttgtcgc
     2221 agatatagat tgggctgcag ttggtgctgg gttaacaagt atctcgatta gttggtctaa
     2281 aagtgacatc gcagcattga ttgccgtcgc tggaaccaca atttctcctt acttattttt
     2341 ctggcaagca gctgaagaaa tggaagaggg cttacaggct ccggacattt cagtcactca
     2401 catacatgca atgcgcggag atgtattcgc cggaatgctc tcaggagttt ttgtaatgtt
     2461 tgcaatcatg gctacaacgg cggccacact tttcggcact ggaataactt caatcacctc
     2521 gcctgagcaa gcggctgcag cactcaagcc tgtggcgggt gagttcgcaa gtgtcttatt
     2581 tgccttaggt attttgggta ccggcttact tgcagtccca gtattagcag gttctactgc
     2641 atatgcagtt agcgaagcat tcgggtggaa agaaagtttg gaacgcaaac ctagcgaagc
     2701 taaacagttc tacgcagtgt tggcggcctc aatgttgatt gcactattac ttaatttgat
     2761 tggagttaat ccaatgcagt cgttaatttt ggcagcgata ataaacggca taactgcgcc
     2821 gatacttatg ttggtaattt ggaaaatttc tcgagaccag acactgctgg gtcaatggaa
     2881 atcgccaatg tggtcatcca cattggtagg cattggaact attgggatgt ttgccttacc
     2941 gatttgctgg ttatttgcta actagcttcc aagcacttgg taacaatagt ccagcagcag
     3001 cagctttgat tatgtcgcca actataaatg gagttacgcc atgggttaat gcccaagcaa
     3061 aaccattacc cgtgaattgg gctagccaaa taaagcccaa taaataaata accgctgacc
     3121 cagcaatgta ggcaagcaca gtcgatctaa tctgagtggt tgcacctcta gatgcaatcg
     3181 aaccaactac aaatgaagcg acaataaatc cgattaggta accaccagta gcaccaaggg
     3241 ctttatcgat accgccggcc ccaccagcta aaactggagc gccagctgcg gctaacgcga
     3301 agtacaaggt ttgcgccaaa acagatctgg tcgcacctaa cgaagctgcg cctacaagta
     3361 cggcgaatgt ttgcaaagta agtgggactg gtgtgaatgg aagtggaatt gctatttgag
     3421 cgctagctgc ggtcacaccg gtgagcccaa aaactaatag aacacttttt gcaattgatc
     3481 tattcaccca ccgatcagcc aacacggcac ttgcggatac tgaactcatt ttgctgctcc
     3541 tacgtttggg cgctatcgcc accaacttcc ctaaagccta ctaatcctgc gctttagtgt
     3601 caggtaggcc aacttggcta attgcaccag cacagtaata gccttaactt caggaggttt
     3661 ggcgtgcgac acataacaag tgaagaaatc gatcaatgcc tagattttcc aaatcttgtt
     3721 aaaagtttgg aaaatgcatt taaatcgcat tggacaactc cagttcgagc gcatcacacc
     3781 gttaccggaa taggccaaga caaagttatg ttgttgatgc c
//
LOCUS       DBNHVN010000147         8694 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1217244, whole genome shotgun sequence.
ACCESSION   DBNHVN010000147 DBNHVN010000000
VERSION     DBNHVN010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 8694)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8694)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8694
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1217244"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="AC16Y1_02760"
     CDS             <1..354
                     /locus_tag="AC16Y1_02760"
                     /inference="COORDINATES: protein motif:HMM:NF014856.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HIR5831417.1"
                     /translation="DGKLAELPELNWKSESAVTVVMAAEGYPEKSVLGAAISGLEDAQ
                     GQGAKVFHAGTALDHTGNVVVNGGRVLSITGLGIDLADARRRAYQAVETIKFAGEHHR
                     TDIALAAVKEEIQIP"
     gene            351..1784
                     /gene="purB"
                     /locus_tag="AC16Y1_02765"
     CDS             351..1784
                     /gene="purB"
                     /locus_tag="AC16Y1_02765"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009679952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR5831418.1"
                     /translation="MSKPSISNVLATRYASSGMANIWSAENKISIERHLWIAVLKAQK
                     DLGIDIPQAAIDAYEKALPNIDLASIAAREKVTRHDVKARIEEFCELSGFEHIHKGMT
                     SRDLTENVEQLQIVEALYFVKIKAVATAAKLAELAVRYTDVALTGRSHNVAAQTTTLG
                     KRFANAAQELLIAIERLDDLLARYPIRGIKGPVGTAQDMLDLFDGDSTKLVELEKRVA
                     DHLGIKNVLTSVGQVYPRSLDYDVISALVQLASGPSSLATTIRLMAGHELVTEGFKEG
                     QVGSSAMPHKMNTRSCERVNGLAVILRGHAAMVGELSGNQWNEGDVFCSVVRRVALPD
                     AFFALDGLLETFLTVLDEFGAFPAVIDRELQRYLPFLATTKILMAAVRNGVGREVAHE
                     AIKEHTVKVALEMRGENGGDGKVILERLAADNRLGLSLDQLTQLIAKPIEFTGVAREQ
                     VSQIAQKVSKLVSENPDSANYRPEPIL"
     gene            1781..2677
                     /locus_tag="AC16Y1_02770"
     CDS             1781..2677
                     /locus_tag="AC16Y1_02770"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR5831419.1"
                     /translation="MIINPVDYQLDETFTHIYSGKVRDLYKTKDGRLLFIASDRISAF
                     DWVLPSTIPDKGRILTAMSLWWFEQLEDIVPNHIITTNVPTQVKGRATYCEELEMFPV
                     ECVARGYLTGSGLSDYQKTSSICGNGLPPGLKDGSQLPKPIFTPATKAALGDHDENVT
                     FDEVMIKIGMEEAQQLKRFTLAVYERALNIAKSRGIILADTKLEFGLGLKGLNKNKVV
                     LADEVLTPDSSRFWPADLWQPGQAQPSFDKQYVRDWLLSKESGWDKNSGEAPPALPDE
                     VIEKTRARYVEAYERLTGEKFS"
     gene            complement(2678..4306)
                     /locus_tag="AC16Y1_02775"
     CDS             complement(2678..4306)
                     /locus_tag="AC16Y1_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019852096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR5831420.1"
                     /translation="MSQSVTKNSQTVAGAILDLLWQAGIRVTFGIPGVHNLAFWDALG
                     VNRPKIIGVRHEQTAAYAADGLARTSGNLAVAITTTGPGAANTVAAFGEAAISGSPIF
                     VIASEAPIAKRGKNGSRGLLHEMDDQAALFAPLAKKLHGIAMAISVEDGTTAVDVAAE
                     MIRDLLRAPAGAGYLGIPADVLGQPFTGSIPKLELGQEVIQVNSASAVEALKDTKKIV
                     LWAGGGATESAEGISALAAHWNAPILTSFAGRGIAADSPFSVKVPIHEPEVENMFSQA
                     DALVVFGSQLDGMNTKNWTIKWPAKIVVVDANPDFVLRNVNADAVITANDIDKVANLL
                     VADTKPQENWFDVSALNNAVRNRLEVDEKSKRGTALVRAIEQNWPSDGVIVCDMAVAG
                     YWTGVYADQPKPRRLVYPVGWGTLGFGLPAAIGPAAAGVRTLSVCGDGGVAFALGELA
                     TIVQENLPYTLLIVDDGGYGMLRYDQIVMGHPERGVDLVSPDWATLAQAFGLSFTETT
                     IEDLGEALSLKQPGIILVREKLNPPKSTSPRWNE"
     gene            4340..5836
                     /locus_tag="AC16Y1_02780"
     CDS             4340..5836
                     /locus_tag="AC16Y1_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5831421.1"
                     /translation="MSTFVSQNPANLSDVVAKVPAATASDIVEAARLAKAAQVAWAKM
                     PAPARGRVIANAGRLFEKNKDALSRLVTREMGKVFREALGDVQEVIDTCDFFLGEGRR
                     LYGQTVPSEMPNKNLFTFRKPVGTALIITAGNFPAAVPSWYIIPALLTGNSVVWKPSE
                     FTPAISKAMTDIFYAAGVPKDVLITVVADGKETFKGLEEGLKQGLINKVGFTGSTEVG
                     RLIGAKVGEYIQSACLELGGKNPMVVMPDANLDLVLDGATFAGFGTAGQRCTSLGTMW
                     LHEDIYDRFLDAYVKRIENEKVGDPMQDVLFGPMIAEKYLDTHEHNLKLVANHHQVHG
                     STGVGKISASNPRKGFVGDPDAGVFAHPTIVSGIKGGDDLYHTETFGPLVGVGKFRTL
                     DEAIELSNGHGYGLSSAIYTSDPNSVWRFREGISAGMVSINNSTSGAEAHLPFGGNGK
                     SGNGSRQSGIWVLDQFTSWQSMNWDWSGKLQKAQMDNEEVPHNPDFTI"
     gene            5848..7014
                     /locus_tag="AC16Y1_02785"
     CDS             5848..7014
                     /locus_tag="AC16Y1_02785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019852098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5831422.1"
                     /translation="MANLPQQVDVVVVGGGVMGASAAFHLAEAGVSVLLVEKNELASG
                     STSKAAGGVRANFSDELNIQMGARSLDLLADFNNRPGQEIDLHRPGYMFALTNADDVT
                     TFERSTELHHKFGVESRMLTPQEAQKISPLLNVDDVLACSFTPNDGHCTPESVVMGYA
                     SGARRHGATVLTNVEATGITVKGDEIVSVTTSAGEVQTSTVINCAGAWSPQIGAHIGL
                     DLPVTPYRRELLITEPLGDEFKDLPASMPMTIDYSTSLYWHREGKGLLMGFSDKTVGP
                     GFDFSRDPKFPEKLAELAYERAPRLLDVGVGSGWSGLYEVTPDHNALLGEWKGVSRFL
                     YATGFSGHGFLEGPAIGEILRDLYLGKTPFVDISPLSVDRFSSGGQLRPEKNIV"
     gene            7014..7688
                     /gene="yczE"
                     /locus_tag="AC16Y1_02790"
     CDS             7014..7688
                     /gene="yczE"
                     /locus_tag="AC16Y1_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YczE"
                     /protein_id="HIR5831423.1"
                     /translation="MATLPAWLKPAKTVPHAPWSAGESNWKAKPRTYATLVLGLWLFG
                     TGEAALVATGAGQSPWTVFAEGISLTTKLSIGWAAFATSLVVLLLWIPLKRKPGAGTI
                     LNAIVISLALEVMIPFFPNPQSGSGQLAQVLFGIALVGFGSALYIPANLGAGPRDGLM
                     TGLHFKTGKPVARIRLFLEVSALSIGWLLGGTVGIGTVLFSLLLGYSVAFWFGVVDRI
                     DQKIKD"
     gene            complement(7702..8670)
                     /locus_tag="AC16Y1_02795"
     CDS             complement(7702..8670)
                     /locus_tag="AC16Y1_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5831424.1"
                     /translation="MSNTAVKHAATGYLLYLAAAILWGFNGTVSKIILLSVEDAARVS
                     QLRTTAAFIILFIFVALTNRKAFRLNRTEVPLLIFYAIVGITFTQWAYFEAISRMPVG
                     ITLLIEFTAPIYIVLWVRYVRKQKVRSTIWLGLAMAIGGLALVAQVWKGFMLDGVGFA
                     FALGATFSLIIYYLVGETASKNRDSVSLIMWGFLFASIFWSVVRPWTSFPWERLDVTV
                     QPFVDSATTTPLLYLITYMVIGGTVLPFVLVVMAIKHLGAAGASLMGMIEPLVAFLVA
                     WVVLNESLNTIQIIGGLAMLTGVYVAELARISKKSDSEKIETIVPS"
BASE COUNT         2451 a         1898 c         1966 g         2379 t
ORIGIN      
        1 gatggaaaac tggccgaact gccagaactc aactggaaat ctgaatctgc cgtaacggtt
       61 gttatggctg cagaaggtta tccagaaaaa tcagttttag gtgcagcaat ttctggcctc
      121 gaggatgcgc aaggccaagg tgcaaaagtt ttccatgccg gcactgcact cgatcacact
      181 ggaaatgttg tagttaatgg tggacgagtg ctgtctataa ctggactcgg aatcgattta
      241 gcagatgcac gtagacgtgc ctatcaagct gttgagacaa ttaagtttgc tggcgaacac
      301 catcgaacag atatcgcact tgccgcagtt aaagaggaga tacaaattcc atgagcaaac
      361 caagtatctc taatgtttta gctacgcgtt atgcctcttc tggcatggcg aatatttggt
      421 cagctgaaaa taaaatttct attgagcgtc acttatggat tgcggtactc aaagcacaaa
      481 aagatttagg tatcgatatt ccacaagctg caatcgatgc atacgaaaaa gcattaccaa
      541 atattgattt agccagtatt gccgcccgag aaaaagtaac tcgacatgat gtcaaagccc
      601 gaatcgaaga attttgtgaa ctttctggat ttgagcacat tcataaggga atgactagcc
      661 gagacttaac cgaaaatgtt gaacaacttc aaatagttga agcactctac tttgtaaaaa
      721 tcaaggccgt tgcaaccgct gctaagttgg ccgaactagc cgttcgctac acagatgtcg
      781 cgttaactgg ccgcagtcac aatgtagcgg cgcaaactac aacgcttggt aaaagatttg
      841 ctaatgcagc ccaagaatta ctaattgcaa ttgaacgatt agacgattta cttgcgcgct
      901 atcccatccg cggaattaaa ggtccagttg gtaccgccca agatatgttg gacttattcg
      961 atggtgattc gaccaagtta gttgaacttg aaaaaagagt tgccgatcac ttagggatta
     1021 agaatgttct aactagtgtg ggccaggttt atccaagatc tcttgattac gacgtaatta
     1081 gcgcactagt tcaacttgca agcggaccaa gttcgttagc caccacaatc cggctaatgg
     1141 ctgggcatga attagtgact gaaggcttca aagaaggcca agtcggttca agtgcgatgc
     1201 cgcacaaaat gaacactcgc tcatgtgagc gcgtcaatgg tcttgccgtt attttgcgcg
     1261 gtcacgctgc tatggttggt gaactttcag gtaaccaatg gaacgaaggc gatgttttct
     1321 gttctgtagt cagacgagtt gcgctgcctg atgcattctt tgcactcgat ggcttattag
     1381 aaactttctt aactgtgctg gatgagtttg gtgcttttcc agcggtgatt gatcgagaat
     1441 tacagcgcta tcttccgttt ttagcaacta caaaaatttt gatggctgct gtacgcaatg
     1501 gtgttggtcg agaagttgct cacgaagcaa ttaaagaaca taccgttaaa gtagctcttg
     1561 aaatgcgcgg tgaaaatggt ggcgatggca aagttatttt agaaagactc gcagctgata
     1621 atcgcttagg tttgtcacta gatcaactaa ctcaattaat tgcaaaaccg attgagttca
     1681 ccggcgtagc gcgagaacag gtttcgcaaa ttgcccaaaa agtttcaaaa ctcgtttctg
     1741 aaaatccaga ttcggctaac tatcgtccgg agccgattct gtgataatta atccagttga
     1801 ttatcaatta gatgaaactt tcactcatat ctattcgggt aaagttcgcg atctatataa
     1861 aacaaaagat ggccgtttac tttttatagc aagcgatcga atctcagcct ttgattgggt
     1921 tttgccaagc actattccag ataaaggccg aatcttgacg gcaatgtcgt tgtggtggtt
     1981 tgagcagtta gaagatattg tgcccaatca cataattaca actaatgtgc cgacgcaagt
     2041 aaaaggccgg gcaacttatt gtgaagaact cgagatgttt ccagtcgagt gtgtagcccg
     2101 tggctaccta actggctcag ggctgtcgga ttatcaaaaa accagttcga tttgtggcaa
     2161 tggacttcca cctggtctta aagatggttc tcaattgcca aaaccaattt ttaccccagc
     2221 cacaaaagct gcacttggtg atcatgatga gaacgtaact tttgacgaag tcatgatcaa
     2281 aattggtatg gaagaggctc aacaactaaa gcgatttacc ttagcggttt atgaacgagc
     2341 attaaatatt gcaaaatctc gtggaattat tttggctgat acaaaacttg aatttggttt
     2401 gggcctaaag ggattaaata aaaacaaagt agtactcgca gatgaagtat taacacctga
     2461 ctcatcaaga ttttggccag cagatttatg gcaaccaggt caggcgcaac cttcgtttga
     2521 taagcaatat gttcgtgatt ggctactttc aaaagaatct ggttgggata aaaactcagg
     2581 tgaggcgccg cctgcattac ccgatgaagt aattgaaaaa acgcgagctc gatatgttga
     2641 agcttacgag cgactaactg gcgaaaaatt tagttaatta ttcattccag cgtgggctgg
     2701 tgcttttcgg tggattaagt ttttcgcgta ccaaaataat cccaggctgt ttaagcgata
     2761 gtgcctcacc caaatcttca atcgtagttt cggtgaacga taaaccaaat gcctgcgcta
     2821 gcgttgccca atctggtgaa actaaatcaa ctccacgctc tggatgcccc ataactattt
     2881 ggtcgtagcg caacatcccg tagccaccgt catcaactat taataaggta tacggcaaat
     2941 tttcttgaac aattgtggcc aattcaccta gggcaaatgc gactccaccg tcgccacata
     3001 ctgaaagcgt tcgcactcca gccgcagctg gaccaattgc agccggcaac ccgaaaccta
     3061 gagtgcccca accaactgga taaaccaatc gtcttggctt tggttgatct gcataaactc
     3121 cagtccaata tccagcaaca gccatgtcac aaacaattac gccatcgctt ggccaattct
     3181 gttcaatagc gcgaacaagt gcagtgccac gttttgattt ttcatcaact tcgagtcggt
     3241 ttcgcactgc gttattaagt gccgaaacat caaaccagtt ttcttgcggc ttggtatctg
     3301 caactaataa attcgcaacc ttatcaatgt cattggcggt aattaccgca tctgcattta
     3361 catttcgcag aacgaaatct ggattagcat ccaccacaac gatttttgcg ggccatttaa
     3421 tagtccagtt tttggtattc atgccatcta gttggctacc aaataccacc agcgcatcgg
     3481 cttggctaaa catgttttcg acttcaggct catgaattgg aacttttaca ctgaacggcg
     3541 aatctgcagc aatacctcgg ccagcgaatg aagtaagaat aggcgcattc cagtgcgcag
     3601 ctaaagcaga tattccttct gcactttcag ttgcgccgcc accagcccaa agcacaatct
     3661 tctttgtgtc ttttaaagcc tcaacagcag aagccgaatt aacttgaatt acttcttgac
     3721 ctaattcgag ttttgggatt gatccagtaa atggctggcc aagcacatcg gctgggattc
     3781 ctaaatagcc cgcacccgct ggtgcacgca ataaatctct gatcatttca gctgcaacat
     3841 caacagcagt agtaccatct tcaacgctga ttgccattgc gatgccatgt aattttttag
     3901 ccaacggagc aaatagtgca gcttgatcat ccatttcgtg gagtaaaccg cgcgatccat
     3961 ttttgccacg ttttgcaatc ggcgcctcag aggcaattac aaaaataggt gagccagaaa
     4021 tcgcggcttc accaaatgcc gcaacggtat ttgccgcgcc tggaccagtt gtcgttatag
     4081 cgacagctaa atttccagaa gttctggcta aaccatctgc agcatatgct gcagtttgtt
     4141 catggcggac cccaataatt tttggacggt taacgccaag tgcatcccaa aatgcaagat
     4201 tatgaacacc aggaatacca aatgtgacac gaatcccagc ctgccaaagg agatccaaga
     4261 tagccccggc tacagtttgc gaatttttgg tgacagactg agacatgtaa atactctagc
     4321 gttttgaaag aggccgaagt tgtctacctt cgtttcccaa aatcccgcca acctatctga
     4381 tgttgttgcc aaagtacctg ctgcaactgc atctgatatt gtcgaggctg cacgcctggc
     4441 taaagctgcg caagttgcgt gggcaaagat gccagcacct gctcgtggcc gcgtgatcgc
     4501 aaatgctggg cggttgtttg agaagaataa agatgctctt tctcgtttag tcaccagaga
     4561 aatgggcaaa gtatttcgcg aagccctagg tgacgttcaa gaagtaattg atacctgtga
     4621 tttcttttta ggtgaaggtc gtcgactcta tggccaaact gtgccaagtg aaatgcctaa
     4681 caagaaccta ttcactttcc gcaagccagt aggcacagca ttaattatta ctgcaggtaa
     4741 tttcccagca gccgtgcctt cttggtacat catcccggcg ttactcaccg gtaattctgt
     4801 ggtttggaaa ccatcagaat tcactccagc tatttctaaa gcaatgacgg acatttttta
     4861 tgcggccggc gtgccaaaag atgtgctcat tactgttgtt gcagatggca aagaaacttt
     4921 taaaggcctt gaagagggtt tgaaacaagg ccttattaat aaggtaggtt tcactggttc
     4981 aactgaagtt ggccgcttaa ttggtgccaa agttggcgaa tacatccaaa gtgcttgcct
     5041 tgaacttggc ggcaagaacc caatggtagt tatgccagat gccaatttag acctagtact
     5101 cgatggcgcc actttcgctg gctttggcac agcgggtcag cgctgtactt cacttggcac
     5161 tatgtggtta cacgaagata tttatgatcg cttcctagat gcatacgtaa agcgcattga
     5221 aaacgaaaaa gtgggcgatc caatgcagga tgtgcttttt ggcccaatga ttgctgaaaa
     5281 atatttagac actcatgaac ataatttaaa gttagtggcc aaccatcatc aggtacatgg
     5341 ttctacaggt gttggaaaaa tttcggcatc aaatccacgc aaaggttttg ttggtgaccc
     5401 tgatgctgga gttttcgcgc acccaacgat tgtttctgga attaaaggtg gagacgatct
     5461 ttatcacact gaaacttttg gtccactagt aggagttgga aagtttagga ctttagatga
     5521 agcaatcgaa ctttcgaacg ggcacggtta tggattatca agtgcgattt acaccagtga
     5581 tccaaattca gtttggcgat tccgtgaggg catttctgcc ggcatggttt cgatcaataa
     5641 ctcaacttca ggtgctgaag cgcacttacc tttcggtgga aatggcaaga gtggaaacgg
     5701 ttctcggcaa agtggtatct gggtacttga tcaattcact agttggcaat caatgaattg
     5761 ggattggtcg ggcaaacttc agaaagctca aatggataat gaagaagttc cgcataaccc
     5821 tgatttcaca atttaggaaa aaattcagtg gctaatcttc cgcagcaagt tgatgtagtt
     5881 gttgttggcg gaggcgtcat gggcgcttcc gctgcgttcc acttggccga agccggcgta
     5941 agtgtattgc tagttgaaaa aaatgagttg gccagtggtt caacttctaa agcagctggt
     6001 ggcgttcggg ccaatttctc agatgaatta aatattcaaa tgggtgctcg ctcattagat
     6061 ttacttgctg acttcaataa tcgtccaggt caagaaattg atttacatcg cccggggtac
     6121 atgttcgcac ttactaatgc agatgatgtc actacgtttg aacgctcaac agaactacat
     6181 cataaatttg gtgttgagtc tcgaatgcta accccacaag aagcccaaaa aataagtccg
     6241 ttattaaatg ttgatgatgt acttgcttgc tcatttacgc caaatgatgg gcactgcaca
     6301 ccagagtcag ttgtgatggg ttacgcatct ggcgcccgtc gtcatggtgc aacagtgctg
     6361 acaaatgtcg aagccactgg aattacggtt aaaggtgacg aaattgttag cgtcacaaca
     6421 agtgctggtg aagtacaaac cagcaccgtt attaactgtg ctggtgcttg gtcaccacaa
     6481 attggtgcgc acataggttt agatttacca gtcactccct atcgccgtga acttctcatt
     6541 accgaaccac tgggagatga gttcaaagat ttaccagcat caatgccaat gacaattgat
     6601 tattcaactt cgctttattg gcatcgtgaa ggtaaaggct tactgatggg cttttcggat
     6661 aaaactgttg gtcctggttt tgatttcagc cgcgatccaa agtttcccga aaaattagct
     6721 gaactggcat atgagcgagc acctcggtta cttgatgtag gcgttggatc aggttggtct
     6781 ggtttgtatg aagttacccc tgaccacaat gcactattgg gtgaatggaa aggtgtttca
     6841 cgttttctct atgcaaccgg ttttagtggc cacggttttc tagaaggtcc agcaattggt
     6901 gaaattttgc gcgacttgta tttgggtaag acaccttttg tcgacattag cccgttgagc
     6961 gttgataggt tttctagcgg cggacaactg cggccagaaa agaatattgt ttagtggcga
     7021 cacttcctgc gtggcttaag ccagcaaaaa cagttccgca tgcaccgtgg agtgctggcg
     7081 aatcaaattg gaaagcaaag cctcgcactt acgcaacact cgtgttgggc ttatggctat
     7141 ttggtaccgg ggaagcagcc ttagttgcga ccggagctgg tcagtcaccg tggacggttt
     7201 ttgcagaagg tatttcgcta actacaaaat taagtattgg ctgggctgca tttgcaacct
     7261 cactcgtagt tttattgctt tggatcccgc ttaaacgcaa accaggtgct ggaacgatac
     7321 tcaacgcgat tgttatctcg cttgcgttag aagttatgat cccctttttc ccaaatccac
     7381 aatccggttc gggtcaacta gctcaagtgc tatttggaat cgccttagtt ggttttggat
     7441 ctgcacttta tatcccggct aatttaggtg ccggaccgcg tgatgggtta atgaccggac
     7501 tgcatttcaa aactggaaaa ccggttgctc gaattcggct atttttagag gtcagtgcac
     7561 tttctattgg ctggttacta ggtggcactg ttggtatcgg taccgttttg ttcagtttgc
     7621 tacttggcta cagcgtggcg ttttggtttg gcgtcgttga tcgaattgat cagaaaatca
     7681 aggattaaaa atttgcctaa attaacttgg cacaattgtt tcgatttttt cactatctga
     7741 ttttttagaa attcttgcta gctcggcaac gtaaacacca gtcagcattg ctaggccacc
     7801 aattatttga attgtattta gtgactcatt aagtactacc caagccacca agaaagcgac
     7861 aagtggttcg atcatgccca tcaaacttgc tccagcagca cctaagtgtt taatcgccat
     7921 aactactaag acgaaaggta ggactgtgcc gccaatgacc atgtaggtaa ttaaatataa
     7981 aagtggtgtg gtcgtcgcac tatcaacaaa aggttgaaca gttacatcaa gtcgttccca
     8041 aggaaaacta gtccaaggac gaactacaga ccaaaagatc gaagcaaata aaaatcccca
     8101 cataatcaat gaaactgagt cacgattttt gcttgccgtt tcaccaacta agtaataaat
     8161 aatcagtgaa aaagttgcgc ctaaggcaaa agcaaatccg acgccgtcta acatgaaccc
     8221 tttccaaact tgggccacca gagctagtcc gccgattgcc attgccaagc ccagccaaat
     8281 tgttgaccta actttttgtt tacgcacgta acgcacccaa agcacgatgt aaataggtgc
     8341 tgtgaattcg attaacaaag tgatgcctac tggcattcgg ctaatggctt cgaaatacgc
     8401 ccactgggta aaggtaattc cgacaattgc gtaaaaaatc aaaagcggca cttcagttct
     8461 atttagccga aaagcttttc gattagttag cgcgacaaaa ataaacaaga taataaatgc
     8521 agcagttgta cgtagttgag aaactcgcgc tgcatcctca acgcttaaca aaataatttt
     8581 gctaactgtc ccgttaaaac cccaaaggat tgctgcagct aaataaagta ggtaaccagt
     8641 cgctgcatgt tttactgcag tattactcaa tctggctcac tttcaaaaac taga
//
LOCUS       DBNHVN010000148         1070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1662581, whole genome shotgun sequence.
ACCESSION   DBNHVN010000148 DBNHVN010000000
VERSION     DBNHVN010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1662581"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="AC16Y1_02800"
     CDS             <1..481
                     /locus_tag="AC16Y1_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831425.1"
                     /translation="HPTDVPGTSNLQKSFALAMRAGIFWFVAGAFIGTSWHLYVGTAL
                     FIAPSYLGLFQDKFPNYPKLYQILPAGLPGLAFTLLVASYSLAGLTAILGETPDLAKL
                     AFVLLPIPSVILSVLGMLGREPADGDVRWYQRDNLTWLYRIGGIAVFIYTLQLTGII"
     gene            630..>1070
                     /locus_tag="AC16Y1_02805"
     CDS             630..>1070
                     /locus_tag="AC16Y1_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011607102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HIR5831426.1"
                     /translation="MSLPPLVSDAADLSVDEVRRYSRHLIIPDVGMSGQKRLKNAKVL
                     CVGAGGLGSPALMYLAAAGVGTLGIIEFDTVDESNLQRQIIHGQSDIGRSKAESARDS
                     VKEINPYVQVNLHELRLDSSNVMDLFAQYDLIVDGTDNFATRYLV"
BASE COUNT          282 a          216 c          243 g          329 t
ORIGIN      
        1 tcacccaacg gatgtgccgg gcacgagtaa tttacaaaaa tcatttgctc ttgcgatgcg
       61 ggcgggtatc ttttggtttg ttgccggtgc atttatcggc acatcatggc acctatatgt
      121 aggtactgca cttttcattg cacctagtta tttagggtta ttccaagata agttcccgaa
      181 ctatccaaag ctttaccaaa tcttgccagc aggcttacca ggcttggctt tcactttgtt
      241 agttgcttcg tattcacttg ctggattaac agccatcctt ggcgaaactc cagacctagc
      301 aaaacttgca tttgttttgt tgccaattcc atcggtaatc ctgagtgtgc ttggcatgct
      361 tggccgtgaa ccagctgatg gtgatgtgcg ttggtatcaa cgcgataact taacttggct
      421 ttatcgcatt ggtggaattg cagtgtttat ttacaccttg caactaaccg gaattattta
      481 aggtatctgc ctaattttcg tgacttctta agtgctgttg caaatccctt gcgataactg
      541 tccaagatca gatcctgcgg gaacaagaaa cataaaaaca cggttggata gtagtagcaa
      601 tcttaaatta tcaatttttg ggagatcgaa tgtctttgcc accactagtg agtgatgctg
      661 cagacttaag tgttgatgaa gtccgtcgat atagtcgaca cctgatcatt ccagatgttg
      721 gtatgtcagg tcagaagcga ttaaagaatg caaaagtttt gtgtgttgga gcaggcggct
      781 taggttctcc agcattgatg tatttagctg cagcaggcgt tggcacactt ggcataattg
      841 agttcgacac agttgatgaa tcaaatttgc agcgtcaaat tattcatggc caaagcgaca
      901 tcggaagatc taaagcagaa tctgcgcgag atagtgttaa agaaattaat ccttacgtcc
      961 aggtaaatct gcacgaactt cggttagatt ccagtaacgt aatggactta tttgcccaat
     1021 acgacttgat tgttgatggc acagataact ttgcaacccg gtatttagta
//
LOCUS       DBNHVN010000149         1909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_620482, whole genome shotgun sequence.
ACCESSION   DBNHVN010000149 DBNHVN010000000
VERSION     DBNHVN010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_620482"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="AC16Y1_02810"
     CDS             complement(<1..698)
                     /locus_tag="AC16Y1_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5831427.1"
                     /translation="MTNSPWLAHYPSEVPKQLPQVSFANLPELLRSAASTYEKTTAFT
                     QVMPNGMNGSLTFAQVDLLSDHFACYLREELGLSAGDRVAVQMPNCLSYPIVAFGIFK
                     AGCVLVNTNPLYTPTEMIHQFRDSGATTLVIIDMFAGRLPEVVGKTNIKNVVTVKISE
                     FFPVVVGGVVRGVQKFWSRSLPKIEVEHTKFSSALSLGKAKYSKDKVSSYSISLTSDT
                     LAALQYTGGTTGVS"
     gene            898..1593
                     /locus_tag="AC16Y1_02815"
     CDS             898..1593
                     /locus_tag="AC16Y1_02815"
                     /inference="COORDINATES: protein motif:HMM:NF015682.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HIR5831428.1"
                     /translation="MSNPAQDLALTNGQNNLLRRLKLVSSTSLADASKSTKPLRMLPP
                     EITPVRPELKLCGRAVTVDASEGLMSMIAALKITVTGDVLVVRGSEIGAVSGELFATE
                     AKRQGLAGIVIDGFCRDRAILLTIDLPVYARGSVPNAPGTSALPVIQIPLTISNVTVR
                     PGDLLVGDADGIVIGSIDEFLAVIDRAEEIEKYEYALREKIANGSPLIDHINYAEHLA
                     ALEAGNESKLNFS"
     gene            1590..>1909
                     /locus_tag="AC16Y1_02820"
     CDS             1590..>1909
                     /locus_tag="AC16Y1_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase-like domain-containing protein"
                     /protein_id="HIR5831429.1"
                     /translation="MTAQIHYSRRMDGVRPSAIRDLLRLGADPEIISFGGGYPDPNLF
                     PIKELQEVYSDLLVPERADALQYTTSNGLPELREQVAKRLTNDGMPCTADDVLIVQGG
                     QQGL"
BASE COUNT          508 a          426 c          477 g          498 t
ORIGIN      
        1 ttagatacgc cggtagttcc gccggtgtac tgcaaggcag ccaaggtatc tgaagtcaga
       61 cttattgagt agctactgac cttgtccttt gaatactttg ccttacctaa ggaaagagct
      121 gaagaaaact ttgtgtgctc aacttcgatt tttggtagcg accgactcca aaacttctga
      181 acacctcgga caactccacc aaccacgact gggaagaact ctgagatttt tacggttaca
      241 acatttttaa tgttggtctt accaacaacc tctggcaaac gtccagcgaa catgtcgata
      301 attaccaatg ttgtagcgcc cgagtctctg aactggtgaa tcatttcggt aggagtgtaa
      361 agcggattgg tatttaccaa aacgcacccg gccttaaaaa taccgaaggc aactattggg
      421 tagctcaaac agtttggcat ttgcacggca acacgatcac ccgcgctaag acctagttct
      481 tcgcgaaggt agcaagcgaa gtgatcagat agtaggtcaa cctgggcaaa agttaaagag
      541 ccattcatgc cgtttggcat tacttgagtg aaggccgtgg tcttttcata agtgctggcc
      601 gcgcttcgaa ggagctctgg caaatttgca aatgaaactt gtggaagctg ctttggaact
      661 tcacttggat agtgagctag ccacggactg tttgtcatct ttgctctttt ccgcaatccc
      721 cagcctggca aaattgccgc agccggactc taatttccac aagcataatg gtctagattc
      781 gccatatctg ggcttcacac cgagatactt caagagtttt ccagacgatg gtggtggggc
      841 tggcagaggc aactttgttg aattgaatta agttaagcaa aaggggttag gggacatatg
      901 tcgaatccag cgcaggacct tgcattaacc aacggtcaga ataatttgct caggcgattg
      961 aagttggtta gcagtacctc gctagccgat gccagtaagt caaccaaacc gcttcgcatg
     1021 ttgccgccgg aaatcactcc agttcgacct gagctgaagc tctgtggtcg ggcagtaact
     1081 gttgatgcca gcgaaggttt aatgtcgatg attgcggctt taaaaattac agtgaccggt
     1141 gatgtgctgg tggttcgcgg aagtgaaatt ggagcggtat ccggtgagct gtttgcaacg
     1201 gaagctaaac gccaaggttt ggccgggata gttattgatg gattctgtcg agatcgcgcg
     1261 attctgctga caatcgattt acctgtttat gcccggggtt cagttccgaa tgcgccggga
     1321 acaagtgcgc ttccagtcat ccaaattccg ttgacaataa gtaatgtgac cgtacggcct
     1381 ggcgatcttt tagtggggga tgccgatggc attgttattg gctcaatcga tgaattcctg
     1441 gccgttattg atcgggcaga agagatcgaa aagtacgaat acgcgcttcg tgaaaaaatt
     1501 gctaatggtt caccgctgat agatcacata aattatgccg agcacttagc tgcactagaa
     1561 gcgggcaacg agagtaaatt gaatttctca tgaccgctca aattcactat tcacgtcgca
     1621 tggatggagt tcgcccatca gctatccgcg atctattacg ccttggtgct gacccagaaa
     1681 taatttcatt tggtggcggt tatccagatc caaacctttt tccgatcaaa gaattacagg
     1741 aagtttattc agatctacta gttccagaac gagctgatgc cctgcaatac acaacctcaa
     1801 acggattgcc agaacttcga gagcaagtgg ccaagcgatt gacaaatgac ggtatgcctt
     1861 gcacggcaga tgatgttttg attgtccaag gtggacaaca aggtttaga
//
LOCUS       DBNHVN010000150        13649 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1217453, whole genome shotgun sequence.
ACCESSION   DBNHVN010000150 DBNHVN010000000
VERSION     DBNHVN010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 13649)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13649)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13649
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1217453"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..753
                     /gene="pyrF"
                     /locus_tag="AC16Y1_02825"
     CDS             70..753
                     /gene="pyrF"
                     /locus_tag="AC16Y1_02825"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR5831430.1"
                     /translation="MVKAPIAVALDAPNLVIAQEWAKAVGPHVQVVKVGLEVFLRDGH
                     ASVQAARDASGCDIFLDLKLHDIPATVNGAAESVSILNPKYLTVHASGGAEMIKAAVD
                     AVPDTLITAVTILTSLTQDQLTQMGWTGSAQDIVKRLAAMSVAAGARAIVCSPQEVAS
                     VRAEVGNAAVLITPGVRPAGSDAGDQKRIATPQQALADGADLLVIGRPITGSSDAGSA
                     AAQIAASLL"
     gene            767..1096
                     /gene="mihF"
                     /locus_tag="AC16Y1_02830"
     CDS             767..1096
                     /gene="mihF"
                     /locus_tag="AC16Y1_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013127201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HIR5831431.1"
                     /translation="MPVPVLTQEQRVAASAKAVVVRVRRAEVKSALKSKVLTLKQLIQ
                     VLESDDIIAGMKVKAVLESLPAIGKIKAKTIMEKCEIAPSRRLKGLGEHQVQALLNEL
                     DSRQVRA"
     gene            1093..1638
                     /gene="gmk"
                     /locus_tag="AC16Y1_02835"
     CDS             1093..1638
                     /gene="gmk"
                     /locus_tag="AC16Y1_02835"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012949164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR5831432.1"
                     /translation="MTAHLVVLAGPSGVGKSSVISQALTQAPETWLSVSATTRAPRPG
                     EVDGVNYFYVSDAIFDEMISNNQLLEWASFAGNRYGTPRKPVEEKLAQGIPVLLEIEL
                     QGAMQIREAMPQAVLVFLEPPTWADLEARLAGRGTESQTQVQARLDAALKELESKGFF
                     DHVIVNDEVNRAAGELVQYLK"
     gene            1686..1946
                     /gene="rpoZ"
                     /locus_tag="AC16Y1_02840"
     CDS             1686..1946
                     /gene="rpoZ"
                     /locus_tag="AC16Y1_02840"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020551792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR5831433.1"
                     /translation="MAAVTPEGITNPPIDELLAVADSKYALVIYAAKRARQINAYYSQ
                     LGEGLLEYVGPLVESGAQEKSLSIALREINEGKLTVEKEVVS"
     gene            1997..3196
                     /gene="coaBC"
                     /locus_tag="AC16Y1_02845"
     CDS             1997..3196
                     /gene="coaBC"
                     /locus_tag="AC16Y1_02845"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR5831434.1"
                     /translation="MNIILGVGGGIAAYKVASLLRLLKEAGHDVHVVPTRAALNFVGL
                     ATWESLSGHPVTSEVWDDVAEVPHVQLGKNANLVIVAPATADLISRTATGSANDLLTN
                     ILLTARCPIVFAPAMHTEMWEHAATQENVKTLRARGITVIDPAVGRLTGSDSGAGRLP
                     EVEQIAQIALSSVTPAKKDLVGKKFLISAGGTREYLDPVRYLGNSSSGKQGIALASAA
                     ADRGAQVTLVCANVVLPTPANVEVIHVQTADELATAVKSGAAKSDVVIMAAAVSDFTP
                     AQRSETKIKKSDSNGKLSIELIQTEDILKTLVASKKPGQYIVGFAAETGDNKATALDY
                     GFAKLASKGCDALVVNEVGNGKGFGTDDNEVVILTSKPKASLPVERSAKYEIAQQVLN
                     VIAKAIG"
     gene            3256..4446
                     /gene="metK"
                     /locus_tag="AC16Y1_02850"
     CDS             3256..4446
                     /gene="metK"
                     /locus_tag="AC16Y1_02850"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR5831435.1"
                     /translation="MSGRLFTSESVTEGHPDKMADQISDAILDDLLAQDPKSRVAVET
                     MLTTGQIHVAGEVTTNGFADVMALVRQVVNEIGYDTSEKGFDGNSCGISVSIGAQSPD
                     IAQGVDDAVEERVSGSADPLDRQGAGDQGLMFGYASDETEALMPLPITLAHRLAEQLS
                     AVRKSGQMGYLRPDGKTQVTIEYDGDQAVRLDTVVVSSQHAPDVDLEKQLTPEVKKFV
                     IDPIVAKFDIDTKDFKVLVNPTGKFVIGGPMGDAGLTGRKIIVDTYGGMARHGGGAFS
                     GKDPSKVDRSAAYAMRWVAKNVVAAGLAKRCEVQVAYAIGKAHPVGVFVETFGTATAP
                     EENIRQAILKVFDLRPAAIIRDLELLNTSVVKYRPTAAYGHFGRPGFTWERTDRAADL
                     QNAV"
     gene            4461..6470
                     /locus_tag="AC16Y1_02855"
     CDS             4461..6470
                     /locus_tag="AC16Y1_02855"
                     /inference="COORDINATES: protein motif:HMM:NF036997.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HIR5831436.1"
                     /translation="MTQDQLSFLPGGAKPTAKPKTKVTKKPKASVPIAKTNPVAQVAI
                     DNALPHLDRLFDYAVPEKLDEIAKPGVRVRVRFAGRLTDGFLISRSETSDHVGELAAL
                     TNVVSPEVVLLPEILELAKDVANRFAGVLSDVLRTAVPNRHAGAEETKFPTRPKLTAP
                     SDSTWAQYSGGAALITRTTQKQTPRAVVTTGQDDPAQLIASYAQSVASHDQGIIVVVP
                     DRTAIDRVLSALTNNGVPKSAIAVLAADDGSATRYRNWLSVLRGSSQIVVGTRSAVFA
                     PVTNLSAIVVWDDWNETHTSPQAPYWNSREVAVLRSSAQECALVLIGAAMSVQSYALQ
                     PWAVHIARTRDDLRKNSPKVRSALDETYLKADPAGKTARVPALVLQVIKEALNTGPVL
                     VMVARTGYTPRLSCANCRELAVCSQCAGPLLQTDRTSSPVCNLCGHLETDWQCSRCNK
                     NSLRAVVIGSTRTAEEFGRAFPGVPVRNSSSDHILRNISGQPSIVVATPGAAPIAETG
                     YSALILLDGSGMLNRQDLDAGQDTYGKWTEFMSLVRPTGQVVVVAESEQPAVQALIRH
                     DPAGFAKHEFESRAQVSLPPTVKLAVLTGTQADIDDLVSQCELPAETVIRGPVPTHDG
                     QMRLLLSVPKKLGLTLSTELKRATAVRSTRKTGGTVNVRIDPDKL"
     gene            6473..7015
                     /gene="def"
                     /locus_tag="AC16Y1_02860"
     CDS             6473..7015
                     /gene="def"
                     /locus_tag="AC16Y1_02860"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR5831437.1"
                     /translation="MAIKPIRLFGDPILTTRADEVTVFDKELRNLVRDLTETMQAAPG
                     AGLAAPQIGVSLRVFTYDVDGVVGHLINPQLDLSEQTQDGPEGCLSLPELTYDCIRAL
                     SVIAKGMNMYGEPVTIEGSDLLARAIQHETDHLDGIVFIDRLDEQLRKEAMKEIRQTE
                     WFGAVPTIKVSPHPMSNGIL"
     gene            7017..7925
                     /gene="fmt"
                     /locus_tag="AC16Y1_02865"
     CDS             7017..7925
                     /gene="fmt"
                     /locus_tag="AC16Y1_02865"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019983893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR5831438.1"
                     /translation="MRIAFAGTPDAAVPTLQALIDSPHEIAFVITRPDAPKGRGQELT
                     PSPVAQLASESGLPIVKTSDLSQVANKVENLDAVVVVAFGGLVPKSLLDIPKHGWINV
                     HFSLLPHWRGAAPVQHSIMAGDDVTGITTFRIEEALDSGPVLAYITTSIDEHETAGQL
                     LDRLSAEGAQLALATLQGLANNSIHPLVQPLDGVSYAPKITTQSARINWQEPALVIAR
                     KIRAMTPSPGAWTTCGDDRYKLTPVKLIPEVTHLAAGQLAFVEDEAYVGTGSHAIALD
                     QIQLPGKTFTQAQNWARNLKSDLVFE"
     gene            7922..9268
                     /locus_tag="AC16Y1_02870"
     CDS             7922..9268
                     /locus_tag="AC16Y1_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR5831439.1"
                     /translation="MSFAARKVAFEVLQAVAVDDAYSNLVLPAAITTAKLDSRDAGFA
                     TELTYGALRRQGSLDAVIKACSERDDIDPKVLTVLRIGTYQLLFMRVPDHAAVSETVD
                     LARKVTSVGPTKFVNAVMRKIAAHDWPTWVAKLTDGLPAIEKLAIEYSYPVWVIRVLA
                     DAYQCDSESIKPILETGNTPIPVSLTARPGQYSVEDLLQLPNVQPGKWSPLAGQIIKS
                     ESGSTKPGDLIPVKENRVGVQDEGSQLVALALVNAKVEQPESHWLDMCAGPGGKAAIL
                     AGYAPMHNADLTAIEPTPARAKLVENSLAHAPGEPKVIVQDAREFESDKKFDRILVDA
                     PCTGLGALRRRPEARWRKNPKDIPPLTSLQSELLAKAATLVKTGGVIGYATCSPHIAE
                     TDAIVNDFLKKHPDFELQDVSSTLPQLNLPAGTKTMRLNPMVHETDGMYLALLTKR"
     gene            9282..9968
                     /gene="rpe"
                     /locus_tag="AC16Y1_02875"
     CDS             9282..9968
                     /gene="rpe"
                     /locus_tag="AC16Y1_02875"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018570184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR5831440.1"
                     /translation="MGLRISPSILSADFGHLVDECERVAPNADWLHVDIMDNHFVPNL
                     TFGLPVVEALIGKVSTPIDCHLMISDADRWAPGYAEAGAKSVTFHVEAAANPKQVAAD
                     IRKAGARAGMAIKPGTKLEDYEELFAHLDMILIMTVEPGFGGQSFMHDQLPKVKRARE
                     VIKNLDLDIWLQVDGGVSAKTIEAAADAGADTFVAGSAVYNTDDPRAAIENLRTLAQK
                     ATDNSWWGNH"
     gene            9968..10972
                     /gene="ribD"
                     /locus_tag="AC16Y1_02880"
     CDS             9968..10972
                     /gene="ribD"
                     /locus_tag="AC16Y1_02880"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR5831441.1"
                     /translation="MSATLPQEKLMQAAIDAAYQVNLAQDINPRVGAVLVNSAGEIVA
                     KGLHRGSGSSHAEIDALNQVTDANDLMLYTTLEPCNSTGVTGPCSQAIVNAGIKKVVI
                     GKLDDNPNMSGGRQYLLDHGVEVISDVLAEACEKINETWHFAHRNNRPFVTWKVATSL
                     DGFIAAKDGSSKWITSPEAREKVQELRASVGAIVTGTGTVIADDPELTVRNVELKNQP
                     LRVVVGTRSIPTKAKVLTGEKPALHICADIKDALTKLWTDFAVHQVLVEAGPGLSRSL
                     WAHGLVDEVFWFQAMLILGDGKHAIGDFGINSLDAGLRFSDYALNRVGLDTLIQFRTN
                     "
     gene            10983..11576
                     /locus_tag="AC16Y1_02885"
     CDS             10983..11576
                     /locus_tag="AC16Y1_02885"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015661852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HIR5831442.1"
                     /translation="MFTGIVEELGQVAKLETLADAVRISITGPLVVSDAKIGDSISVN
                     GVCLTVTQKTSDSFSADVMQETLNRSTLGKLAPGSKVNLERPVTLATRLGGHLVQGHV
                     DGLGKVISREKSDNWDVVTIEPNPELLKYIVEKGSITVDGTSLTVASVNHQVFTVSLI
                     PETLTKTTLGLVNAGDQVNLEVDVMAKYVEKLMAAQK"
     gene            11573..12808
                     /locus_tag="AC16Y1_02890"
     CDS             11573..12808
                     /locus_tag="AC16Y1_02890"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006236659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HIR5831443.1"
                     /translation="MTTALNSVTLDSIESAIADIAAGKMVVVVDDEDRENEGDLIFAA
                     SKSTPELVGFMVRHSSGVICVPMEGPDLDRLGLPPMTHINEDRKGTAYSVSVDARDGI
                     ETGISAKDRSHTIKVISDSATEPWEITRPGHVFPLRAMAGGVLRRPGHTEAAVDLARL
                     AGLKPAGVICEVVHDDGSMMRAPQLREFANEHGLKMISIADLIKYRRRNESLIKRVAT
                     AKLPTEFGEFDIVGYESILDGTDHVALVMGDLGDGQNVLVRVHSECLTGDALGSLRCD
                     CGPQLRAAMRKVASEGRGVVLYVRGHEGRGIGLLHKLAAYQLQDGGADTVSANTALGL
                     PADSRDYGTGAQILVDLGIKSMRLLTNNPTKRAGLDGYGLTITDSVPIEIEPNDHNLA
                     YLQAKRDQMGHTLSFGDTK"
     gene            12805..13278
                     /gene="ribH"
                     /locus_tag="AC16Y1_02895"
     CDS             12805..13278
                     /gene="ribH"
                     /locus_tag="AC16Y1_02895"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR5831444.1"
                     /translation="MSATGSPEDKVTGFTGKRVVIVASSWHNQIMDALIAGATRVVTE
                     AGVTAEVHRVPGTFELPIACRAAAKSGADAVIALGVVIRGETPHFDYVCQAATDGILQ
                     VSLETGVPVGFGVLTCDDEKQALDRAGLPGSKEDKGAQAAAAALNTWVTLKNISS"
     gene            13371..>13649
                     /locus_tag="AC16Y1_02900"
     CDS             13371..>13649
                     /locus_tag="AC16Y1_02900"
                     /inference="COORDINATES: protein motif:HMM:NF018837.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HIR5831445.1"
                     /translation="MNDAVIPNSDFANDDGSADPLLRRALKSYTESATAESARNLLDI
                     LCEARLLVPVVAVLDTEGVNDAGLKFDKDSHMRSVEFTSNDGRTAVLAF"
BASE COUNT         3736 a         2992 c         3365 g         3556 t
ORIGIN      
        1 aacttcgaac tgaacttgaa gtgcgcggct ttgcgaaatt aactgatgcg attggagcag
       61 cacatcaaaa tggttaaagc cccgattgcg gtcgctctgg atgcgccaaa cttagttatc
      121 gcccaagagt gggctaaagc cgtaggtcca catgttcaag tggtcaaagt tggcttggaa
      181 gtttttttgc gcgatggcca tgccagtgtg caagcagcac gtgatgcatc tggttgtgat
      241 atttttcttg accttaaatt gcacgacatc ccggcaacgg taaacggggc agccgaaagc
      301 gtatcaatcc ttaatccaaa atatttaact gtccatgcat ccggcggcgc agagatgatc
      361 aaagccgcag tcgatgccgt gccagatacc ttaattactg ccgtaacaat tttgacttca
      421 ttaactcaag atcaactaac tcaaatgggt tggactggat cagcacaaga tattgttaag
      481 cgacttgctg ccatgtcagt agctgcaggt gctcgcgcaa tcgtgtgctc accgcaagaa
      541 gtcgcaagtg tgcgcgccga agttggaaat gcggcagtac ttatcacccc tggtgttcgc
      601 ccagctggca gtgatgccgg cgatcaaaag cgaattgcaa ctccgcaaca agcgcttgct
      661 gatggtgctg acttactcgt gatcggacgg ccaatcactg gcagcagtga tgctggaagt
      721 gcggccgcac aaatcgccgc tagcctgctc tagtaacatt tggcctatgc cagttccggt
      781 actcacccaa gaacaacgtg tagcagcaag tgctaaagca gttgtggttc gagttcgccg
      841 agctgaagtt aaatcagcat taaaatcaaa agtcttaact ttaaagcaat taattcaagt
      901 gttagagtca gacgacatca ttgccggtat gaaagttaaa gcagttttag aaagcttgcc
      961 ggcaattggc aaaattaaag ccaaaaccat tatggaaaaa tgcgaaatcg cgccatcacg
     1021 taggttaaaa ggtcttggcg aacatcaggt tcaagcacta cttaatgaac tagatagtcg
     1081 ccaggtgcga gcatgacggc acatttagtg gtacttgctg gccctagcgg tgttggtaaa
     1141 tcgtcggtta tctcgcaagc attaactcaa gcaccagaaa cttggttgtc agtttcagca
     1201 actacccgag caccgcggcc aggcgaagtc gatggcgtta attattttta tgttagcgat
     1261 gcaatttttg atgaaatgat ctcgaataat cagttgctcg agtgggccag ttttgccgga
     1321 aatagatatg gcacgccacg aaaaccagtt gaagaaaaat tggcgcaagg tattccagtt
     1381 cttttagaaa tagaacttca aggggcaatg caaatccgcg aagcaatgcc acaagcagtc
     1441 ttagttttcc ttgaaccacc aacttgggct gacctcgaag cacgcttggc cgggcggggg
     1501 actgagtcgc aaacacaagt acaagcgcgc cttgatgctg cgcttaaaga gttggaatct
     1561 aaagggtttt tcgatcacgt catcgtcaat gacgaggtaa atcgggcggc tggcgaattg
     1621 gtacaatatc taaagtaatt ttcgaagtta cagtgaaaat cataaatttt taaaggagtc
     1681 agcacgtggc tgcagtaaca ccagaaggca tcaccaatcc accgattgat gaactattag
     1741 cagttgctga ctcaaaatac gctttagtta tctacgcagc aaagcgcgct cgccagatta
     1801 atgcttacta ctcacaactt ggtgaaggcc tacttgaata tgtaggacca cttgttgaat
     1861 ctggcgccca agaaaaatca ctctcaattg cattacgtga aatcaatgaa ggcaaactaa
     1921 ctgttgaaaa ggaagttgtt tcctaaacag tttggctact agccacataa ctgccaggta
     1981 ggtgtaattc acttaaatga atatcatcct tggcgttggc ggtggaatcg cagcttacaa
     2041 agtagctagt ctgctacgcc tactaaaaga agccggacat gatgttcatg ttgtcccaac
     2101 tcgcgctgcc ttaaattttg tcggcctagc aacttgggaa tcactatctg gccatccagt
     2161 aaccagtgaa gtttgggatg acgttgccga agttcctcat gtacaacttg gtaaaaatgc
     2221 caacttagtt attgtggcgc cagcaacagc agatttaatt tcacgcaccg ctactggtag
     2281 cgccaatgac ttgctcacca acatactttt aactgcgcgc tgtcccattg tttttgcccc
     2341 agcaatgcac actgaaatgt gggaacatgc cgccacgcaa gaaaatgtaa aaactcttag
     2401 agctcgcggg attaccgtta ttgatcctgc cgttggtcgc ctgactggaa gtgacagtgg
     2461 cgctggtcga ctacctgaag ttgaacaaat tgctcaaatt gctttgtcga gcgttacacc
     2521 tgcgaaaaaa gatttagttg gaaaaaagtt tttaattagc gccggtggca cgcgtgaata
     2581 tcttgatcca gttcgctatc tcggaaatag ttcaagtggc aaacaaggta ttgcgctagc
     2641 cagtgcagca gcagatcgcg gtgcccaggt gactttagtt tgcgccaatg tagttttgcc
     2701 cacgccagcc aatgttgaag tgattcatgt tcagactgcg gacgaacttg caactgctgt
     2761 taagtcaggg gcggccaagt ctgatgtagt aatcatggcg gcggcagttt ctgatttcac
     2821 gcccgcccaa cgcagcgaaa ccaaaatcaa gaaatctgat tccaatggca aactttccat
     2881 tgaattaatt caaactgaag acattttgaa aactttggtg gccagcaaaa agcctggcca
     2941 atacattgtg ggcttcgctg ccgaaaccgg tgacaataaa gcaaccgctc ttgattacgg
     3001 ctttgcaaag ttagccagca aaggctgtga cgcacttgtt gtgaatgaag tcggtaatgg
     3061 caaaggcttt ggcactgatg acaatgaagt tgtgatccta accagtaagc ccaaagcaag
     3121 tctgccggtt gagcgcagcg ctaaatatga gattgcccag caggtactta acgtaatcgc
     3181 taaggccatc ggctaaacta aacaagcaaa tttcctatag aagcctctgt agcactaaaa
     3241 gtaaaggtgt catacatgtc cggtcgtctt tttacctctg aatctgtaac cgaaggtcat
     3301 ccagacaaaa tggcagatca gatttcagat gcaattcttg atgacttatt agcccaggat
     3361 ccaaaaagcc gcgtagctgt cgaaactatg ttgaccactg gacaaataca tgtggctggc
     3421 gaagtaacaa ccaatggctt tgcagatgtg atggcgttag ttcgccaagt tgtaaatgaa
     3481 attggttatg acacatctga aaaaggtttt gatggaaata gttgcggtat ttcagtttcg
     3541 atcggtgctc agtctccaga tatcgcccaa ggtgtagacg atgcagttga agaacgcgtt
     3601 tctggttcgg cagatccact agatcgccaa ggtgctggcg accaaggttt gatgtttggt
     3661 tatgcaagtg atgaaacaga ggcgcttatg ccactgccga ttactttggc acatcgctta
     3721 gcagaacagc taagtgcagt tcgcaagagt ggccaaatgg gttacttacg gccagatgga
     3781 aaaactcaag taactatcga atatgacggt gatcaggcag ttcgactaga cactgttgta
     3841 gtttcatcac agcatgcacc tgatgtcgat ttagaaaagc aactaactcc agaagttaaa
     3901 aagtttgtca ttgatccgat tgtggcaaag tttgatattg atactaaaga cttcaaagtt
     3961 ttagttaacc caactggcaa attcgtaatc ggtggaccaa tgggtgacgc tggcttaaca
     4021 ggtcgcaaaa ttattgtgga tacttacggc ggtatggctc gtcacggcgg tggcgcattc
     4081 tcgggtaaag atccgtcaaa agttgaccgc tctgccgctt atgcaatgcg ttgggttgct
     4141 aagaacgttg ttgcagctgg acttgctaag cgctgtgagg tccaagttgc ttacgcaatt
     4201 ggtaaagcac atccagttgg cgtttttgta gaaacatttg gcactgcaac ggcacccgaa
     4261 gaaaacattc gccaagcaat tttgaaggtt ttcgatttgc gtccagccgc gattattcgc
     4321 gaccttgaac tgctaaatac gtcagttgtg aaataccgac caacagcggc ttatggccac
     4381 tttggtcgcc caggctttac ctgggagcgc acagatcgtg ctgctgattt acagaacgcc
     4441 gtctaagtaa tcttggagtt gtgactcagg accagctaag ttttttgcca ggcggcgcaa
     4501 agccaactgc aaagccaaaa accaaagtaa ctaagaagcc aaaagcttcg gtcccgatcg
     4561 caaaaactaa tccagtcgcg caagtagcaa ttgataacgc gctaccgcat ttagatcgac
     4621 tctttgatta cgccgttcca gaaaaactag atgagatcgc aaaaccaggt gttcgggtgc
     4681 gggttcgctt tgctgggcgg ctaaccgacg gttttttaat atctcgaagt gaaactagcg
     4741 atcatgttgg cgaacttgct gcacttacta acgttgtttc acccgaagtg gtgctacttc
     4801 cagaaatact cgagctggca aaagatgtgg caaatagatt tgctggagtt ttatctgatg
     4861 ttttgcgcac ggcagttcca aatagacatg ccggcgcgga agaaactaaa ttcccaactc
     4921 ggcccaagtt aactgcgcca agtgattcaa cttgggcgca atacagcggt ggggcagcgc
     4981 taattacacg caccacacag aaacaaacac cgcgcgctgt tgtaacaaca ggccaagatg
     5041 atccagctca actaatcgct tcgtatgcac agagcgtggc aagtcacgat caaggcatta
     5101 tcgtcgtggt gccagatcgc actgcaatcg atcgcgtttt gagtgcgctg actaataatg
     5161 gtgtacctaa gtcagctatc gccgtgttag ctgcagacga tggatccgca actcgatatc
     5221 gcaattggct ctcagttttg cgcggtagca gccagatagt agttggcact agatctgctg
     5281 tgtttgctcc ggtcacaaac ttgtcggcga ttgtggtttg ggatgactgg aatgaaacgc
     5341 acacaagtcc gcaagcccct tactggaata gtcgcgaagt tgccgtgtta cgaagtagtg
     5401 ctcaagagtg cgctttagtt ttaatcggcg ccgcaatgtc agtacaaagt tacgccttgc
     5461 agccgtgggc agtgcatatt gcgcgaactc gtgatgattt acgcaagaac tcaccaaaag
     5521 ttcgaagtgc acttgatgaa acttatttaa aagctgaccc ggcaggtaaa actgcgcggg
     5581 ttccagcgct ggttttgcaa gttattaaag aagctcttaa tactggccca gttttagtaa
     5641 tggttgcgcg cactggttac acccccagac tttcgtgtgc gaattgtcga gaacttgcgg
     5701 tgtgttctca gtgtgctggc ccactgttac aaactgatcg aacaagtagc ccagtttgta
     5761 atttatgcgg acatcttgaa actgattggc aatgttcgcg ctgtaataaa aatagtttgc
     5821 gtgcagttgt gattggcagc acccgaacag ccgaagaatt tggtcgagca tttcctggtg
     5881 tgccggtgcg taactcttca agcgatcaca tcttgcgcaa catttctggt cagccgtcga
     5941 ttgtggtggc aactccagga gccgcgccga tcgccgagac tggttacagt gcactgatcc
     6001 tgctggatgg atctggaatg ttaaatcgcc aagatttaga tgcgggtcag gatacatatg
     6061 gcaagtggac tgaattcatg tcacttgtca gacctactgg ccaagtcgtt gtagttgctg
     6121 aaagtgaaca acccgcagtg caagcgctta tccgtcatga tccagctgga tttgctaaac
     6181 atgaatttga atcacgggca caagtttcgc taccaccaac tgtgaagtta gccgtcttaa
     6241 ctggaacgca agcagatata gatgatttag tttcgcaatg tgagttaccg gctgaaactg
     6301 tgattcgtgg cccagttcca acgcatgatg gccagatgcg cttgctatta agtgtgccca
     6361 agaaacttgg cttaactctg tcaactgaat tgaagcgcgc gactgcagtg cgcagcacgc
     6421 gcaaaacggg cggaacagtt aatgttcgaa ttgatccaga taaactttaa ctatggcaat
     6481 caaaccaatt aggttattcg gcgatccgat tttaactacg cgcgctgatg aagtaactgt
     6541 gtttgataaa gaattgcgca atttagttcg ggacttaact gaaactatgc aagccgcacc
     6601 tggcgctgga ctagccgcac cacaaatcgg cgtatctctt cgggtcttca cctatgacgt
     6661 ggacggggta gtgggacatt taattaatcc acaattggat ttatctgagc agacccaaga
     6721 cgggcccgag ggatgtctat ctttgcccga gttaacttat gactgtattc gcgctttaag
     6781 tgttatcgct aaagggatga acatgtacgg cgagccagta actattgaag gctcggattt
     6841 actggcccgc gcaattcagc atgaaactga tcatttagac ggcatcgtat ttattgatcg
     6901 tcttgatgaa cagctgcgta aagaggcaat gaaagaaatc cgccaaacag aatggttcgg
     6961 cgctgtgcca acaataaagg taagcccgca tccaatgtcg aacggaatcc tttaacgtgc
     7021 ggattgcatt tgctggtacc cccgatgcag ccgtgccaac tttgcaggcc ctaattgatt
     7081 caccgcacga aattgctttt gtaattactc ggccagatgc cccaaaaggt cgcggccaag
     7141 aattaacgcc aagtccagtg gcgcaattag caagtgagtc tggcttacca attgttaaaa
     7201 cttcggattt aagccaagta gcaaataaag ttgaaaactt agatgcggta gttgtggtcg
     7261 cgtttggcgg attagtgcca aagagtttgt tggatattcc taaacatggc tggattaatg
     7321 tgcatttttc gttgctgcca cattggcgcg gcgctgctcc ggtacaacat tcaattatgg
     7381 ctggcgatga tgttacgggg attactacat ttcgaattga agaagcatta gatagcggac
     7441 cagtgcttgc ctacataact acttcaattg acgagcatga aactgcgggg cagttacttg
     7501 atcgacttag tgctgagggt gcgcagttag cgttagctac cttgcaggga ttagcgaata
     7561 attcaataca tcctctagtt caaccgcttg acggagttag ttacgcacca aaaataacga
     7621 cacaaagtgc gcggataaat tggcaagagc ccgcccttgt tattgctcgt aaaatccgag
     7681 cgatgacccc tagcccaggt gcgtggacaa cttgtggcga tgatcgctac aaattaacgc
     7741 cagtgaaatt aattccggaa gtaactcact tagcagcagg gcagttagca ttcgttgaag
     7801 acgaagctta tgttggcact ggttcgcacg ctattgcgtt agaccagatt cagttacctg
     7861 gcaaaacatt tacccaggca caaaactggg cacgtaacct gaaatcagat ttggttttcg
     7921 agtgagtttt gccgcccgca aagtagcttt tgaagtttta caagccgttg ccgttgacga
     7981 tgcttacagt aatttagttt tgcccgccgc aataactacc gccaaactcg atagtcgtga
     8041 tgccggtttt gctactgagt taacttatgg cgcattacgt cgacaaggca gtcttgatgc
     8101 agtgatcaaa gcctgtagcg agcgtgatga tattgatcca aaagtcttaa cagttttgcg
     8161 catcgggact tatcaacttt tatttatgcg cgtacctgat catgcggcag tttcagaaac
     8221 cgtagattta gcgcgcaagg taactagtgt tgggccaact aaatttgtaa acgctgtgat
     8281 gcgcaaaatt gcggcacatg attggccaac ctgggtagca aagttaactg acggattacc
     8341 ggccattgag aagttagcca ttgaatactc atacccagtg tgggttattc gtgttttagc
     8401 cgatgcctac cagtgcgata gcgagtcgat taaacctatt ttggaaaccg gtaatacgcc
     8461 catcccagtt agtttgactg ctcggcctgg gcaatattca gttgaagatt tactgcaatt
     8521 accaaatgtg caaccaggta aatggtcacc gttggctggc cagattatta aatctgaatc
     8581 tggctcgacc aaacctggtg atttaatccc agttaaagaa aatcgagttg gggtgcaaga
     8641 cgaaggcagt cagctagttg cacttgctct agtaaatgca aaagttgaac aacctgaatc
     8701 acattggcta gatatgtgtg ctggacctgg tggcaaagcg gcaattttgg ctgggtatgc
     8761 gccgatgcat aatgctgact taactgccat cgaaccaaca ccagcgcggg caaagttagt
     8821 tgagaactca ttggcgcatg caccaggtga acctaaagtt attgtgcaag atgcgcgaga
     8881 atttgagagt gacaagaagt ttgatcgaat tttagttgat gcaccatgta ctggcctagg
     8941 tgcacttcgt cgtcgccccg aagccagatg gcgaaagaat ccaaaagaca ttccaccgct
     9001 aacttcatta caaagtgagt tactggctaa agctgccaca ttagttaaaa ccggtggggt
     9061 aattggctac gcaacttgct cacctcacat cgcagaaacc gacgcaattg tgaatgactt
     9121 tttaaagaag catccagatt ttgagttaca agatgtttcg agcacattac cgcaactcaa
     9181 tttgcctgct ggaacgaaaa ctatgcgact aaacccaatg gttcatgaaa ccgatggcat
     9241 gtatttggcg ctacttacta aacggtaagt aggcttaagg tgtgggtttg cgcatatcac
     9301 caagtatttt gtcggctgac tttggtcatt tagtcgatga atgtgaacgg gttgcgccaa
     9361 atgccgattg gttacatgtc gacattatgg ataaccactt cgtaccgaac ttaacttttg
     9421 gcttgccagt agttgaagcc ctaatcggca aagtaagtac cccaatcgat tgtcacttaa
     9481 tgattagtga tgctgatcgt tgggctccgg ggtatgccga agctggtgcc aagagcgtaa
     9541 ctttccatgt tgaggctgcc gcaaatccaa aacaagttgc tgctgatatt cgcaaagctg
     9601 gcgcacgagc cgggatggca attaaaccag gcaccaaatt agaagactat gaagagctct
     9661 ttgcgcatct agacatgatt ttgatcatga cggtcgagcc aggttttggc ggtcaatcat
     9721 tcatgcatga tcaactacct aaagttaaac gcgcccgcga agtgattaag aatcttgatt
     9781 tagatatttg gttgcaagtt gatggcggtg tttccgcaaa gacaattgaa gcggcagccg
     9841 atgctggcgc tgacacattt gtcgctggct ctgcggttta caacactgat gacccgcgtg
     9901 ccgcaattga aaacttacga actcttgccc aaaaagctac tgacaattca tggtggggta
     9961 accactaatg tcggctactt tgccacaaga aaagttaatg caagctgcta tcgatgctgc
    10021 ttaccaagtt aatttagctc aagatattaa tcctcgagtt ggtgcagtct tagtaaatag
    10081 tgctggcgaa attgtggcta agggtttaca tcgaggttct ggaagctcac acgccgaaat
    10141 tgatgcgcta aaccaagtaa ccgacgcaaa cgatttgatg ttgtacacca cacttgaacc
    10201 ttgtaattca actggagtca ctggcccatg tagccaagcg attgtgaacg ctggaattaa
    10261 aaaagttgtg atcggcaaac ttgatgacaa tccaaacatg tcagggggcc gtcagtacct
    10321 acttgatcat ggcgttgaag ttataagtga tgtgctagcc gaggcttgcg aaaaaattaa
    10381 cgaaacctgg catttcgccc atcgaaacaa tcgccctttt gtaacttgga aagttgctac
    10441 ctcacttgat ggatttatag cggctaagga tggttcaagt aagtggatca caagcccaga
    10501 agcgcgcgaa aaagttcaag aacttcgagc aagtgttggc gcaattgtga ctggaacggg
    10561 aacagtaatc gctgatgatc ccgaactaac tgtgcgaaat gttgaactaa aaaatcagcc
    10621 actccgtgtc gtggtgggta ctcgatcaat tccaactaag gcaaaagttt taaccgggga
    10681 aaagcccgcg ctacacattt gcgctgatat taaagatgcg ctaacaaaac tttggactga
    10741 ctttgctgtg caccaggttt tagtcgaggc tggtcctggg ctatcgcgtt cgctttgggc
    10801 gcatggctta gttgatgaag ttttctggtt ccaagcgatg ctgattttgg gtgatggaaa
    10861 gcacgcaata ggggattttg gcattaattc acttgatgcc ggattacgat tttccgatta
    10921 tgcgctaaat cgtgttggat tagatacgtt aatccaattt cgcactaact aaggtttaaa
    10981 aattgttcac cggcattgtc gaagagctgg gccaagttgc caaacttgag accttggctg
    11041 atgccgtgcg catttcaatc accggacctt tagtagtcag tgatgcaaaa attggcgatt
    11101 cgatttcagt taacggtgtt tgtttaaccg tgacacaaaa aacttcagat tcatttagtg
    11161 ctgatgtaat gcaagaaacc ctaaaccgct caaccttggg caagttagca cctggcagca
    11221 aagtaaatct tgagcgccca gttactttgg ctactcgttt aggtggacac ttagtacaag
    11281 gtcatgttga tggactgggc aaagtaattt cacgcgaaaa gtcagacaat tgggatgtgg
    11341 taaccattga acctaatcct gaattactta aatacattgt cgaaaagggc agtattactg
    11401 tcgatggcac atcactaact gtggctagcg ttaatcacca agttttcact gtttcgttaa
    11461 ttcctgaaac gttgactaaa acgactcttg gtttagtgaa tgctggtgac caggtcaatc
    11521 tcgaagttga tgtgatggct aaatatgtcg aaaaattaat ggcggcgcaa aaatgacaac
    11581 cgcattaaat tcagtcacct tagattcaat tgagtcagca attgcagata ttgcagccgg
    11641 caaaatggta gtcgttgttg acgatgaaga tcgcgaaaac gaaggcgatt taatttttgc
    11701 cgcaagtaaa tcaactccag aactagttgg tttcatggtt cgccattcat caggcgtgat
    11761 ttgcgtgcca atggaagggc ctgatttaga tcgcctcggg ttaccgccaa tgactcacat
    11821 taatgaagat cgtaaaggta ctgcttattc agtcagcgtt gatgctcgtg acggaattga
    11881 aaccggtatc agtgccaaag atcgttcaca cacaatcaaa gtgatttcag atagtgccac
    11941 cgagccatgg gaaattacta gaccaggtca cgttttccca ttacgcgcga tggccggtgg
    12001 cgtattacgt cgccccggac ataccgaagc agctgttgat ttagctcggt tagctggctt
    12061 aaaaccagca ggcgtgattt gtgaagtagt tcatgacgat ggttcgatga tgcgcgcacc
    12121 gcaattgcgt gagtttgcaa acgagcacgg actaaaaatg atttcgattg cggacttaat
    12181 aaaatatcgc cgccgaaatg agtctttgat taaacgagtt gccaccgcaa agttacctac
    12241 agagttcggt gaattcgaca ttgtgggtta cgaaagtatt ttggatggta ccgaccacgt
    12301 ggctttggtg atgggcgatc ttggtgatgg tcaaaacgtt ttagttcggg tgcactctga
    12361 atgtctaacc ggtgacgctc ttggttcatt gcgttgtgat tgtggacccc aacttagggc
    12421 cgcgatgcgc aaagttgcca gtgaaggtcg cggggtagtg ctttacgtac gtggccatga
    12481 aggccgcggt attggattac tacataaact tgcggcttat caattacaag atggtggcgc
    12541 ggatactgtt tcggccaata ctgcacttgg tttgccagct gattcacgcg actacggcac
    12601 cggcgcacaa atcttggttg acttaggaat taaatcgatg cggttactta ctaacaatcc
    12661 aacaaagcgt gctggtcttg atgggtacgg cttaactatt actgactcag taccgatcga
    12721 aattgaacct aacgaccata acttggctta tcttcaagcc aagcgcgatc aaatgggtca
    12781 cacgttaagt tttggagata ccaagtgagc gcaaccggca gtcccgaaga caaagtaact
    12841 ggcttcactg gaaaacgagt agtaatagtt gcttcgtctt ggcataacca aatcatggat
    12901 gcgctaattg ccggagccac tcgagttgtt actgaggctg gcgttactgc cgaagtacac
    12961 cgcgtacctg gaacttttga gttaccgatc gcttgtcgcg ctgctgctaa atcaggcgct
    13021 gatgcagtta ttgcacttgg tgttgtaatt aggggcgaga ctccgcactt tgattatgtt
    13081 tgccaagctg ctaccgacgg catcttgcaa gtttcactgg aaactggcgt accagttgga
    13141 tttggcgttt taacgtgtga tgatgaaaaa caagcacttg atcgtgctgg cttaccaggc
    13201 agcaaagaag ataaaggcgc acaagccgca gctgctgcac ttaatacatg ggtgacgtta
    13261 aagaatattt cgtcctaagt acactttttt agtacttatt cgacttaacg atttggcatt
    13321 tttgtgagcg acttttttca agcgaggcct gcaaggatcg agcgcaatat atgaacgacg
    13381 cggtaattcc caatagcgat tttgccaatg atgatggcag tgcagatcct ttattacgtc
    13441 gcgccttaaa gagttacacc gaaagtgcca cggcagagtc tgcacgaaat ttactcgaca
    13501 tcttgtgtga agccaggttg ctcgttccag tggttgcagt cttggatacc gaaggcgtta
    13561 atgatgctgg actcaaattc gataaagatt cacatatgcg ttcggttgag ttcacttcaa
    13621 atgatggtcg cactgccgtg ttagcgttc
//
LOCUS       DBNHVN010000151         2573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_29479, whole genome shotgun sequence.
ACCESSION   DBNHVN010000151 DBNHVN010000000
VERSION     DBNHVN010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_29479"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2533
                     /locus_tag="AC16Y1_02905"
     CDS             <1..2533
                     /locus_tag="AC16Y1_02905"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR5831446.1"
                     /translation="IRLVVFTTLAGSLSGALLPTPAVAQDAINPQEFSTLSIPMSPTN
                     TRWAYQLINSASANSAGFTGSGVRVALLDNGIDHRVTSISSKVIARFDATNSVSGQND
                     HGTATSSIVAADPMPEAGIGGVAPGASILDVRVCLNSGCQTKSIIAGLKWAIDNGANV
                     ISMSLGGGQTIEPAVAHLIRTAISQGIVVVAAAGNNACTATYESGGQTRARNCTQNSL
                     PMSYPGSNSIPGLITVGAIGKDLTRNSYSNYGAYVDVVAPGTDVATFYPWGPNAYFAG
                     TSAATPIVAGIAALIKQAAPNVTSEQVQAILQLTTTDPVIATPNVWESCTYTNNLWDC
                     QNLAPARWPTRYYTGAGVVNAQSAVAMANLLNSGAFKTGLLTSSQDSAINLDWTNSGL
                     GSGPYQVLIDGKQFSETSNTSFQATGLTNSSQYAIQIADAAGNKTVPTIGTAGTPSIY
                     QAPVISTPQVYANAVYLNVASEPNFSKGVLLLANGDQATCARSGSSLRFDCDYIIPIN
                     PIVGTFKLVDDQGNFSTESNQLSFNYSGLSATDSVNLNVVSETEVNASWTAITGASHY
                     CYYDAGIGAWLSTTNTSVQITGVKPGLPQTFTVFASNSTCGPTGLYSPTFWYLPFGPA
                     LTAPNGLTLSNLSSNGVEINFNAPYGADRYAVYRSDGKNWTTGAGSMNVSDSFASDDS
                     GKTFTYWITAIDDVQYGSQYGAISGPFNVSVPMPTVQVINNPPAVAPAPAVGRSAPLT
                     SGSGLSFIPPSSITINPKLRKNSISLVPAARKVKPGNSIWVTGRSRSGVKVSILVNGP
                     CELISRSGNRVKIRGTAPGSCQIYASALANKKFNAAERYLTIPVR"
BASE COUNT          739 a          559 c          556 g          719 t
ORIGIN      
        1 tattagatta gtggttttta ctacactagc cggctcactc tcaggtgcct tgttaccaac
       61 acctgcagtt gctcaagatg caattaaccc tcaggaattt tcaaccctat ctatacctat
      121 gtcgccaacg aatactcgct gggcctacca attaataaat tctgcctcag caaattcggc
      181 tggattcact ggaagtggag tccgagttgc gcttctcgat aatgggattg atcatcgtgt
      241 gactagcatt tcatccaagg taattgctcg gtttgatgca acgaattcag tttcaggaca
      301 aaacgatcat gggacggcaa cctctagcat tgtggctgct gacccaatgc cagaagctgg
      361 tattggcggt gtggcaccag gtgcatcaat tcttgatgtt cgcgtgtgcc taaattcagg
      421 ttgccaaact aaatcgatca ttgctggttt gaaatgggcc atagataacg gtgcaaatgt
      481 aataagcatg agcctgggcg gtgggcaaac tattgaaccc gcagtagctc acctaattag
      541 aactgcaatt tcccaaggga tagttgttgt tgcagctgcc ggcaataatg catgtactgc
      601 cacttacgaa agtggggggc aaactcgggc aagaaactgc acacaaaatt ctctgccaat
      661 gagctatcca ggttcaaatt caattccggg attgattact gtcggtgcaa tcggtaaaga
      721 cttgaccaga aatagttact ctaattacgg cgcttatgta gatgttgttg cacctggcac
      781 agatgtagcc actttttatc catggggacc caatgcctat ttcgctggaa cttctgccgc
      841 aactccaata gttgctggaa ttgcagcctt gataaaacaa gcagccccaa atgtaactag
      901 cgagcaagta caggcaattt tgcaattgac tacaactgat ccagttattg caacaccgaa
      961 tgtatgggaa agttgcacct atacaaataa cctttgggat tgccaaaatt tagcgccggc
     1021 caggtggcca actcgctatt acaccggggc aggtgtggtt aatgctcaaa gtgcagtcgc
     1081 tatggcaaat ttactcaata gtggcgcgtt caaaacaggg ttattaactt caagtcaaga
     1141 tagtgcgatc aatcttgatt ggaccaattc tggattaggt tcaggaccct accaagtatt
     1201 aattgatggt aaacaatttt cagagacttc gaacactagt tttcaagcaa ctggcttaac
     1261 taattcttcg caatatgcaa ttcaaattgc agatgctgct ggaaataaga ctgtaccgac
     1321 aataggtact gcaggaacac cttcaattta tcaagcgcct gtgatctcta cacctcaggt
     1381 ttatgcaaat gccgtttacc tgaatgtcgc aagtgagcca aatttttcaa aaggcgtttt
     1441 gctattagca aatggtgacc aagcaacatg tgctcgaagt ggttcaagcc ttaggtttga
     1501 ttgtgactac ataattccga taaatccaat tgtgggaaca tttaaattgg ttgatgacca
     1561 aggaaacttc agtacagaat caaatcaact ttctttcaat tattcggggt tatcagccac
     1621 agatagtgtg aatttaaatg tagttagtga aactgaggta aacgctagct ggactgcaat
     1681 tactggagct agtcattact gttactacga cgcaggaatc ggagcctggc tatctacaac
     1741 taacacttca gtacaaataa ccggagtaaa accaggccta cctcaaacat ttactgtatt
     1801 tgcaagtaac tccacttgtg gtccgactgg actctactcc ccaacgtttt ggtatctacc
     1861 gttcggccca gcattaacag caccaaatgg attgactctg agtaatttgt cgtcaaatgg
     1921 tgtcgaaatt aatttcaatg caccttatgg ggcagatcgt tatgccgttt atagatctga
     1981 tggaaaaaac tggactactg gcgccggctc aatgaatgtg tccgactcct ttgcttctga
     2041 tgactctggt aagactttta cctactggat aactgcaatc gacgacgttc aatatggctc
     2101 acaatacgga gctatttcag gcccctttaa tgtaagtgtc ccgatgccaa ctgttcaagt
     2161 aattaataat cctcctgcag ttgcacccgc tcctgctgtt ggacgaagtg cgccattaac
     2221 tagcggttct gggttgagtt tcattccgcc aagttctata actattaatc caaaactaag
     2281 aaaaaattct atttccctag ttcctgccgc gcgaaaagtg aaacctggta actcaatttg
     2341 ggtcaccggt agatctcggt ctggagttaa agtttccatt ttggtaaacg gaccttgcga
     2401 gttaattagt cgatcaggaa atcgggtaaa gattcgaggc acggctccag gaagttgtca
     2461 gatctatgcg tcagcattag caaataaaaa atttaatgct gctgagagat atttaaccat
     2521 tccagttaga tgacaacaag acaattagtt ctacatgcta gttaataaac ttg
//
LOCUS       DBNHVN010000152         1600 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1291740, whole genome shotgun sequence.
ACCESSION   DBNHVN010000152 DBNHVN010000000
VERSION     DBNHVN010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1291740"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="AC16Y1_02910"
     CDS             <1..812
                     /locus_tag="AC16Y1_02910"
                     /inference="COORDINATES: protein motif:HMM:NF036799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta-related
                     protein"
                     /protein_id="HIR5831447.1"
                     /translation="EQPMLRTTLLPGLFGAVTRNTSRGFTELGLFEISSVVLPDKKQS
                     ATGTTNPPRPAVTGRPSDKELAKLEELLPAQPLYVAGVLTDADWAKAIEVPLSLANEI
                     GVTLTVQKADIAPWHPGRCAQLVLGDQVIGTAGELTPRVIENVGLPKRTVAFELDLSA
                     LIASANTTPDAPRVWTFPIAKEDLAFVVKKDVPAASLLTEVKAAAGALLEEIKIFDVY
                     EGNQVPAGHKSVAFALRFRASDRTLSAEEVATARNNAIDAANKKFGATLRA"
     gene            909..>1600
                     /gene="argC"
                     /locus_tag="AC16Y1_02915"
     CDS             909..>1600
                     /gene="argC"
                     /locus_tag="AC16Y1_02915"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018785108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR5831448.1"
                     /translation="MKTVAIAGASGYAGAELIRIIHGHPNFELTSITAGSNTGSTLGE
                     VHPQFAAVSELAELKFAPTTLDNLQADLIFFALPHGQSAELISKLPSDQYVVDLGADF
                     RLRDHQQWEKYYGGSHAGAFTYGLPELGQRDEISQAKKVANPGCYATAIELGLAPILK
                     STGTKVHDIIVVAASGTSGAGRNAKVNLLGSEVMGSMSPYKVGGTHQHTPEIEQMVQD
                     FAGGAVEINFTP"
BASE COUNT          430 a          390 c          375 g          405 t
ORIGIN      
        1 atgagcaacc aatgttgcgc actactttgt tgcctggctt atttggcgcg gtaactcgca
       61 atacttctcg tggctttact gaacttggct tatttgaaat ttcttcggtt gtgttgccag
      121 ataaaaaaca aagtgcaact ggcacaacta atccgccacg accagcagtt accggccgac
      181 caagtgataa agaattagcc aaactcgaag agttattgcc agcccaaccg ttgtatgttg
      241 ctggtgtact caccgacgca gattgggcaa aagcaatcga agttccactt tcacttgcta
      301 atgaaattgg cgtaacgctt accgtgcaaa aagctgacat cgcgccttgg catccaggtc
      361 gttgtgcaca attagtttta ggcgatcaag taattggtac cgctggtgaa ttaactccac
      421 gcgtgattga aaatgttggt ttaccaaaac gcacagtggc attcgaactt gatttatctg
      481 cgcttatcgc ttcagccaac accacaccag atgcgcctag agtttggact ttcccgattg
      541 ctaaagaaga tttagctttc gtcgttaaaa aagacgttcc agctgccagc ctgctaaccg
      601 aagttaaggc tgccgctggt gcattacttg aagaaatcaa aatcttcgat gtctacgaag
      661 gcaaccaagt gccagccggg cataagtcag ttgctttcgc attacgtttc cgtgccagcg
      721 accggacact ttctgccgaa gaagtggcca ctgctcgcaa taacgcaatc gatgcggcta
      781 acaagaaatt cggcgccacc ctgcgcgctt aaagtttttg cgactgaaga ctgagccgaa
      841 ggcgatggat cgaagcgcaa taaatatcgc gatttaggcg atcggctatt catccctata
      901 tttaaggtat gaaaaccgta gccattgctg gagcatctgg ttacgcaggc gctgaattaa
      961 ttcgaataat tcacggccat ccaaattttg aactaacttc cattacggct ggctcgaata
     1021 ccggtagcac ccttggtgaa gtgcatccac aatttgctgc cgtaagtgaa ctcgcagagc
     1081 taaagtttgc gccaaccacg ctagacaatc tgcaagctga cttaattttc tttgcgctac
     1141 cgcatggcca atcagccgaa cttatttcga aattacctag cgatcagtat gtagtcgatc
     1201 taggtgctga cttccgacta cgtgatcacc agcagtggga aaagtattac ggcggttcac
     1261 atgctggtgc atttacttat gggctacctg aacttggcca gcgggatgaa attagccaag
     1321 ccaaaaaagt tgctaaccca gggtgctatg ccacggcaat cgaacttggc ctggcgccga
     1381 tcttgaagtc aactggtacc aaagtgcacg acattattgt ggtggcagcc tctggcacat
     1441 ctggtgctgg ccgaaatgcc aaagtaaatc tgcttggtag tgaagttatg ggatcaatgt
     1501 caccgtacaa agtcggtggc acacaccagc acactccaga gatcgagcaa atggttcaag
     1561 attttgccgg cggtgctgtt gaaattaact tcaccccaat
//
LOCUS       DBNHVN010000153         4907 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_919626, whole genome shotgun sequence.
ACCESSION   DBNHVN010000153 DBNHVN010000000
VERSION     DBNHVN010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4907)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4907)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4907
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_919626"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="AC16Y1_02920"
     CDS             <1..585
                     /locus_tag="AC16Y1_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831449.1"
                     /translation="AALLAGCATTDKAGAAAIVGGTKIPSSLVTTQVNEVRVDIENTP
                     PEYLQDLPTMTLLSQMVIDRLVLEEILKFAITDLKIEVTDAQLGEYRDFVFRNYGEPE
                     VKAQLATRNGVAEKYVDGYIYAIMVQREIMDELAPGMPEQVQSAALYKYLSGIADELK
                     VEVSPRYGTWNSEVMQTVLGENFLSTSNEIQQVQ"
     gene            627..1253
                     /locus_tag="AC16Y1_02925"
     CDS             627..1253
                     /locus_tag="AC16Y1_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HIR5831450.1"
                     /translation="MDQLRSPGGCPWDAEQTHQSLAKYLLEETYEALDAMDQGDLVSL
                     REELGDLLLQVVFHARIAQEQDPTFDLDAIAQGVVDKLIRRHPHVFAGLEVNSTAELE
                     ANWATIKSSEKSRESVTDGVPTAMPALQLATQLIYRARENEIKPVEQDLVRQVQDLVG
                     PVTEQSIAQILLAVVELARTKDVDPESALRSASMQYRQVIRRSEGLNS"
     gene            1298..2575
                     /gene="eno"
                     /locus_tag="AC16Y1_02930"
     CDS             1298..2575
                     /gene="eno"
                     /locus_tag="AC16Y1_02930"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR5831451.1"
                     /translation="MASIDGIRAREILDSRGNPTVEVEVLLDDESVGRAAVPSGASTG
                     AFEAVELRDGEKRYLGKGVTKAVRAVEDKIMPELLGLDATEQRLIDQTMIDLDGTANK
                     ANLGANAILGVSLAVAKAAAVSTDLPLYRYVGGPNAALLPVPMMNIINGGAHADSDVD
                     FQEFMIAPIGAPSFAESLRTGSEVYHSLKSVLKAKGYSTGLGDEGGFAPNLPSNRAAL
                     ELISEAIEKAGYKVGKDIAMAMDVAATEFFKDGKYHVEGQVKTTEQMIEFYTQLINDF
                     PIVSIEDPVAEDDWNSWIAFTAAVGDRVQIVGDDHYVTNTERIARGIELKSANALLVK
                     VNQIGSLTETAEAVEMSHRAGFKTMMSHRSGETEDNTIADLAVAFNCGQIKTGAPARS
                     DRVAKYNQLLRIEEDLAEAGRFAGRDAFPRFKG"
     gene            2594..3025
                     /locus_tag="AC16Y1_02935"
     CDS             2594..3025
                     /locus_tag="AC16Y1_02935"
                     /inference="COORDINATES: protein motif:HMM:NF016839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIR5831452.1"
                     /translation="MAKKVKLNSRAFVFIGASFLVLLMLAVPIRSLLQEQREIAALKS
                     QLATQQEKIAALENRKARFADPAYVATLARSRLNYVFPGEIGFIVLDEQTTTAIETVP
                     GAFVPNDDSAWYSKLWRSTQLADNPPAENDPLVVQTEGMPQ"
     gene            3041..3538
                     /locus_tag="AC16Y1_02940"
     CDS             3041..3538
                     /locus_tag="AC16Y1_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010473391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HIR5831453.1"
                     /translation="MLNQEDTAAIAAQINRQPRGVLEVAHRCGCGAPDVIKTSPRLPD
                     GTPFPTMYYLTCPRAASYIGTLEADGVMKQFEDELAADPQLAAQYQKAHDAYLAQREA
                     IEHVEEIAGITAGGMPDRVKCLHVLVAHSLAVGPGVNPLGDKALAMLKDWAIPGPCWV
                     TGDNS"
     gene            3535..4458
                     /locus_tag="AC16Y1_02945"
     CDS             3535..4458
                     /locus_tag="AC16Y1_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019159121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR5831454.1"
                     /translation="MRVGAIDCGTNSIRLLIADIENGQLTDVVRQMIIVRLGEGVDKT
                     GEFSPAALERTFAAVDEYAELIELHKPQRLRFVATSASRDVSNRDVFVAGIKARLGIE
                     PSVISGDEEARLSFLGATADLVNDATPPAIPYLVVDIGGGSTEFVLGADKPVQALSTN
                     VGCVRMTERHLVGDPPTAEQIAAATNDIDAAIEQAAKVVDLASAKSLIGLAGSVTTVA
                     ALALGLKAYKSEVIHGSRITAAQVHKVATELLAMPRAQRAELGPMHPGRIDVIGGGAL
                     VLDRIMQKTGINEVVVSERDILDGIARDLTK"
     gene            4486..4558
                     /locus_tag="AC16Y1_02950"
     tRNA            4486..4558
                     /locus_tag="AC16Y1_02950"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4519..4521,aa:Leu,seq:taa)
     gene            4610..>4907
                     /locus_tag="AC16Y1_02955"
     CDS             4610..>4907
                     /locus_tag="AC16Y1_02955"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018821493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HIR5831455.1"
                     /translation="MAELHLIFGTDIRGPFPDGTESIYLGMGCFWGAEKKLWVAPGVV
                     STSVGYMGGHKENPLYPEVCTGKTGHAEIVRVVFDPSVTTAYDILKIFWENHDPT"
BASE COUNT         1392 a         1022 c         1197 g         1296 t
ORIGIN      
        1 gcggctttgc tggcaggttg tgcaactact gataaagctg gcgcagctgc aattgttggt
       61 ggcaccaaaa ttccatcgag cctagtgact actcaagtta atgaagtccg ggtagacatc
      121 gaaaatactc caccagaata tttacaagat ctaccaacta tgactttgct cagtcagatg
      181 gttattgatc gtttagtttt agaagaaata cttaaatttg caattaccga cttaaaaatt
      241 gaagttacag atgcgcagtt aggcgaatac cgtgacttcg tatttcgcaa ctatggcgaa
      301 cccgaagtta aggctcagtt agctacgcgc aacggggtag cagaaaaata cgtcgatggt
      361 tacatttacg ccatcatggt gcaacgcgaa attatggatg agttagcccc gggtatgcct
      421 gaacaagtgc aatctgctgc tctttataag tacctatccg gaatcgccga tgaattaaaa
      481 gttgaggttt cacctcgcta tggaacttgg aattcagaag taatgcaaac ggtattaggt
      541 gagaatttcc tatccaccag taatgaaata caacaagttc agtaataaat tagatgacct
      601 cacctgttga acgcttggtt caggtaatgg accaattacg ctcaccaggt ggttgtccgt
      661 gggatgccga acaaactcat caaagtttgg ctaaatattt acttgaagaa acctacgaag
      721 cacttgatgc catggatcaa ggtgacttag tttcacttcg tgaagaattg ggcgatttgt
      781 tactccaggt agtttttcat gctcgaatcg cacaagaaca agatccaact tttgatttag
      841 atgcaatcgc tcagggtgtt gtagataaat taattcggcg ccacccacat gttttcgctg
      901 ggttagaagt taattcaact gccgaacttg aagccaattg ggcaacgatt aagtcatcag
      961 aaaaatctcg cgaatcggta actgatggtg taccaacagc tatgccagcc ttgcagttag
     1021 caacgcaact tatttaccga gctagggaaa atgaaatcaa acctgttgaa caagatttgg
     1081 tgagacaggt tcaagacttg gttggcccgg tcactgaaca atcaatcgca cagattttat
     1141 tggcagtagt tgagttggcc cgaacaaaag atgtcgatcc agaatctgct cttcgtagtg
     1201 cgagtatgca gtatcgccaa gtaatccgta ggtccgaggg ccttaacagt tagtcagtag
     1261 aattaaccag taattaagga ttaacgggga gttacaagtg gcaagtattg atggaatccg
     1321 cgcccgcgaa attttagatt ctcgtggcaa tcccacagtt gaagtcgaag tattactcga
     1381 tgatgaaagc gttggccggg ctgcagttcc atctggtgca tcaactggcg catttgaagc
     1441 cgttgaattg cgcgatggag aaaagcgtta tttaggtaaa ggcgttacca aagcagttcg
     1501 cgcagttgaa gacaaaatta tgcctgagct tcttgggctt gatgcaactg aacagcgact
     1561 aatcgatcaa accatgattg atttagatgg cactgctaat aaagcaaact taggtgcgaa
     1621 cgcgatactt ggtgtttcgc tagccgtagc aaaagctgct gcggtcagca ctgacttacc
     1681 gctttaccgt tacgttggcg ggccaaacgc tgcgctactt ccagttccga tgatgaacat
     1741 tattaatggc ggagcacatg cagatagcga cgttgacttt caagaattca tgattgcccc
     1801 aatcggcgct ccatcatttg ccgaaagctt gcgcacaggt agcgaggttt atcactcact
     1861 aaagagtgtg cttaaagcaa agggttactc aactggctta ggtgacgaag gtggatttgc
     1921 accaaattta cctagtaacc gtgctgcact tgaattgatc tccgaagcaa ttgaaaaagc
     1981 tggctacaaa gttggtaaag acattgcgat ggcgatggat gtagcggcaa cagaattttt
     2041 caaagatggt aagtatcacg ttgaaggtca agttaaaaca actgaacaaa tgatcgagtt
     2101 ctatacccaa ttgatcaacg atttcccaat tgtttcgatc gaagatccag ttgccgaaga
     2161 tgactggaat tcctggattg cattcaccgc tgcagttggc gatcgagtgc aaattgttgg
     2221 tgatgatcac tatgtaacaa atactgagcg cattgcccga ggtattgaac ttaaatctgc
     2281 aaatgcgctg ttagtaaaag tgaatcagat tggctcatta actgaaactg ccgaagccgt
     2341 tgaaatgtct catcgtgcag gattcaaaac aatgatgagc caccgttctg gagaaactga
     2401 agacaacacc attgctgact tagcagtggc atttaactgt ggtcagatta aaactggtgc
     2461 accagcacgt tcagaccgcg tagctaagta caaccagtta cttcggattg aagaagacct
     2521 tgctgaagca ggccgctttg ccggtcgcga cgcattccca agattcaagg gttaattaag
     2581 gcagacttaa tacgtggcta aaaaagttaa attaaatagc cgggcatttg ttttcatcgg
     2641 agcaagtttc ttggtgttac ttatgctcgc ggtgccaatc agatcgctgc ttcaagagca
     2701 acgtgaaatt gctgcgctta aatctcaatt agctacccaa caagaaaaaa tcgcggcttt
     2761 agaaaatcgc aaagctagat ttgctgatcc ggcttatgtc gcaactttgg cccgttcacg
     2821 ccttaactat gttttccctg gcgaaatcgg ttttattgtg cttgatgagc aaaccactac
     2881 cgctattgaa actgtacctg gcgcgtttgt accaaacgat gattcagcct ggtattcaaa
     2941 gctttggcgc agtacgcagt tagccgataa tccaccggca gaaaatgatc cgttagttgt
     3001 acaaaccgaa ggtatgccgc aataggcatg caggtttaaa ttgttaaatc aagaagatac
     3061 ggcagcaatt gccgcgcaaa taaatcgaca gccgcgggga gttcttgaag ttgcgcatcg
     3121 atgtggttgc ggggcaccag atgtaattaa aacttcacca cggctaccgg atggcactcc
     3181 atttccaacg atgtattact taacctgccc gcgagctgct tcctacattg gaacccttga
     3241 agccgatggc gtgatgaagc aatttgaaga tgaactagct gctgatccac agcttgctgc
     3301 ccaatatcaa aaagcacatg atgcttattt agcgcagcgt gaggctattg aacatgttga
     3361 agaaattgct ggaatcacgg ctggcggtat gccagaccga gttaagtgcc ttcatgttct
     3421 cgttgcgcat tcattagctg ttggtccagg tgtaaatcct ctaggagata aggcgctagc
     3481 tatgctcaaa gactgggcga tacctggccc atgttgggtg actggagata actcatgaga
     3541 gttggcgcaa tagattgcgg gacaaattca attcgcttgc tgattgcaga cattgaaaat
     3601 ggccaattaa ctgacgtagt ccggcaaatg atcatcgttc gcctcggcga aggcgttgac
     3661 aaaaccggag aattttcacc ggctgcactc gagcgaactt ttgctgcagt tgatgagtat
     3721 gcagagttaa tcgaattgca taaaccacaa cgcctgcgct ttgttgcaac tagtgcaagt
     3781 cgtgatgttt caaaccgaga tgtttttgtc gctgggatta aagctagatt gggtattgaa
     3841 ccaagtgtga ttagtggcga cgaagaagcc cgactttcat ttttaggtgc tactgcagac
     3901 ttagttaatg atgcaacgcc accagcaatc ccttatttag ttgttgatat cggtggtggc
     3961 tcaactgaat ttgttttagg tgctgataaa ccagtgcaag ctttgagtac gaatgttggt
     4021 tgtgtgcgaa tgaccgaacg ccatttagtt ggtgatccac cgacggctga gcaaattgca
     4081 gcggcaacta atgacataga tgcagcaatt gagcaagctg caaaagtagt tgacttagct
     4141 agcgctaaaa gtttgattgg tttagctggt tcagtaacta ctgttgccgc cttggcatta
     4201 ggactgaaag cctataaatc tgaagtgata catggcagtc gaattactgc agcacaagtg
     4261 cataaagttg ccactgagtt acttgcgatg ccacgtgcac agcgcgcaga attaggaccg
     4321 atgcatccag ggcgaataga tgtaatcggt ggcggggcac tggtacttga tcgcattatg
     4381 caaaagactg gaatcaacga agtggtcgta tctgagcgcg atattttgga tggcattgcc
     4441 cgcgatttaa ctaaataggc aagcgtctaa taatctagag gtcttgcccc tgtagtccaa
     4501 tggcagagac aaacgactta aaatcgttac agtgtcggtt cgaatccgac caggggtact
     4561 cttaagaaca cttagggtcc actgaatcgt cagttgaaat gagttaacaa tggccgagtt
     4621 acatttaatt tttggcaccg acatccgtgg tccatttcca gatggcactg aatctatata
     4681 ccttgggatg ggttgtttct ggggtgccga gaaaaaactt tgggttgcac ccggagtagt
     4741 ttctacttca gttggctaca tgggtgggca taaagagaat ccgctttacc cagaagtttg
     4801 tactggtaaa acagggcacg ctgaaatagt gcgagtagtt tttgatccga gcgttaccac
     4861 ggcctatgac atcctgaaga ttttctggga gaaccatgat ccaactc
//
LOCUS       DBNHVN010000154         5783 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1441350, whole genome shotgun sequence.
ACCESSION   DBNHVN010000154 DBNHVN010000000
VERSION     DBNHVN010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5783)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5783
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1441350"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /locus_tag="AC16Y1_02960"
     CDS             complement(<1..982)
                     /locus_tag="AC16Y1_02960"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019066896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR5831456.1"
                     /translation="MGISESGFPVKPVYRSTDQGDWDPEVALGEPGQYPFTRGVYSSM
                     YTGRPWTMRQYAGFGSAAESNRRYHELIKAGSTGLSVAFDLPTQMGYDSDHELAHGEV
                     GKVGVAIDSIDDMRELFDGISLDQISTSMTINAPAATLLLMYQIISEEKGIPGSALTG
                     TIQNDVLKEYISRGTYIYPPNQSLRLITDIFKYCNSELPKWNTISISGYHMSEAGATP
                     AQEVAFTLANGIEYVRAAIKAGLAIDDFAPRLAFFFVARTVLLEEVAKFRAARRIWAR
                     IMKTEFGAKDPKSQMLRFHTQTAGVQLAAQQPEVNLMRITVQALAAIFGGT"
     gene            1082..1468
                     /gene="sdhC"
                     /locus_tag="AC16Y1_02965"
     CDS             1082..1468
                     /gene="sdhC"
                     /locus_tag="AC16Y1_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020496142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HIR5831457.1"
                     /translation="MASTNGTLYRGREGMWSWVAHRITGVAVFFFLFVHVLDTALVRV
                     SPDAYDRVIDTYKTPIVNLLEVGLVGAVLFHALNGIRLILVDFWSKGTKYHRQMLWVI
                     VGIWVAVMIPGTYFMMIHTIEAMFGK"
     gene            1471..1878
                     /locus_tag="AC16Y1_02970"
     CDS             1471..1878
                     /locus_tag="AC16Y1_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020496141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase hydrophobic membrane
                     anchor subunit"
                     /protein_id="HIR5831458.1"
                     /translation="MSTKMPEALLAPRSSRSGRTNYELYSWLFMRISGVVLVFLVLGH
                     LFIMNILDGGVQRINFAFVAGRWSSPFWQVWDLTQLWLALLHGGNGLRTIINDYAERD
                     NTRVVLKSLLYVSTVFILMLGTLVIFTFDPNIG"
     gene            1893..3638
                     /gene="sdhA"
                     /locus_tag="AC16Y1_02975"
     CDS             1893..3638
                     /gene="sdhA"
                     /locus_tag="AC16Y1_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005165557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HIR5831459.1"
                     /translation="MQTHKYDVVIVGAGGAGMRAALESSTRARTAVLTKLYPTRSHTG
                     AAQGGMCAALANVEEDNWEWHTFDTVKGGDYLVDQDAAEVMCKEAIDAVIDLEKMGLP
                     FNRTPEGKIDQRRFGGHTRNHGEAAVRRSCFAADRTGHMILQTLYQNCIKNDVDFFNE
                     FYVLDLLLADDEQGNPITSGVVAYELATGEIHVFQAKSVVLATGGFGKVFKTTSNAHT
                     LTGDGMGIVWRKGLPLEDMEFYQFHPTGLAGLGVLLTEGARGEGGILRNASGERFMER
                     YAPTIKDLAPRDMVARAMVQEVREGRGAGPNKDYVYLDLTHLPAEQIEAKLPDITEFS
                     RTYLGVDPIKERVPVFPTAHYAMGGVPTNVETQVLRNNTDIVPGLFAAGEVACVSVHG
                     ANRLGTNSLLDINVFGRRAGIYAAEHALNSDFVELPENPSAVVEALVNNLKESDGTER
                     IAAIRKELQETMDLNAQVYRNDETLNKALSDIQVLKERYKNVGIHDKGKRYNTDLLEA
                     IELGFLLELAEVLVVSALARKETRGGHAREDYQTRDDEKFMKHTMAYPDPKNGVRLDY
                     KPVTMTRYEPMERKY"
     gene            3680..4450
                     /locus_tag="AC16Y1_02980"
     CDS             3680..4450
                     /locus_tag="AC16Y1_02980"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018188787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HIR5831460.1"
                     /translation="MNAVDPGVINASVSTNSDAPKPFTVTVTVRRFDPEVDQDPFWQD
                     FQVEVFSTDRILDALHKIKWEQDGTLSFRRSCAHGICGSDAMRINGRNRLACKTLIKD
                     LDATKPITVEAIKGLPLEKDLIVDMEPFFAAYREVMPFLIPKGHEPSRERVQSQEERA
                     RFDDSTKCILCAACTSSCPVFWTDNQYFGPAAIVGAHRFIFDSRDAAAETRLEILNSK
                     EGVWRCRTTFNCTEACPRGIQITHAIAEVKQALIRGKI"
     gene            complement(4447..5100)
                     /locus_tag="AC16Y1_02985"
     CDS             complement(4447..5100)
                     /locus_tag="AC16Y1_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HIR5831461.1"
                     /translation="MSWDVVLFDLDGTLTDSGLGVGNGVLYAIREMGFPEPENGELRK
                     YLGPPLWTSFRDFAGMDEAQTKLAVELYRKYYNETGAYENSVYPGIPELLTELNKKER
                     RLAVATSKVDYAAVNILRHFNLDHHFDVIAGGDEHGVHRGTKALVIEHALAELSMCDG
                     ASIVMIGDREHDVHGAAEHKLPAIGVSWGYAEEGELAKAGAIDVVDSVDQLANLLFS"
     gene            complement(5108..>5783)
                     /gene="trpS"
                     /locus_tag="AC16Y1_02990"
     CDS             complement(5108..>5783)
                     /gene="trpS"
                     /locus_tag="AC16Y1_02990"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015746605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR5831462.1"
                     /translation="RMTQFKDKSAKGGAASSSVGLLTYPILQAADILLYQANQVPVGE
                     DQRQHLELTRDLALRFNNRFGEVFTVPEPFIVKETAKIMDLQEPTAKMSKSSPGGCIN
                     LLDPIKATEKKFKSAVTDSETEIKFDQTKKPGVSNLLLINSAYSGKSVSQLVNDFSGR
                     MYGDLKQETAQVVISFIEPYQARVQELLTEKAELEKLMHAGAAKAQQIAEKTLTTVYE
                     KVGFAR"
BASE COUNT         1576 a         1251 c         1293 g         1663 t
ORIGIN      
        1 gagtgccacc aaatattgcc gccaatgctt gaactgtaat tcgcatcaga tttacttctg
       61 gttgttgagc tgctaattgc acaccagctg tttgggtgtg gaaacgaagc atctggcttt
      121 ttggatcttt ggcgccgaat tcagttttca ttattcgcgc ccaaattctt cttgccgcac
      181 gaaacttagc aacttcttct aataaaacag ttctggccac aaagaaaaaa gctaatcgtg
      241 gcgcaaaatc atcaatcgct aaaccggctt taattgccgc gcgcacatac tcaattccgt
      301 ttgccaaagt gaatgcaact tcttgtgccg gtgtcgcacc cgcttcactc atgtgatatc
      361 ccgaaattga aattgtgttc cactttggta attcggaatt acagtattta aagatgtcag
      421 taattaaacg taacgactgg ttcggtggat aaatataggt gccacgagaa atgtactctt
      481 taagcacatc gttttgaatt gttccagtga gagcgcttcc gggaattcct ttttcttcag
      541 aaataatttg atacatcaat agcaaagttg cggcgggtgc attgattgtc atcgaggtag
      601 aaatttgatc gagtgagatt ccatcaaaaa gttctcgcat gtcatcaatc gaatcgatag
      661 cgacgcctac ttttccaact tcaccatgtg ctaactcgtg atccgagtca tatcccattt
      721 gagttggtaa atcgaaagca acgcttaacc cagttgaacc agctttaatt agttcgtggt
      781 accgccgatt tgattcggca gcgctaccaa atccagcata ttggcgcata gtccatgggc
      841 gcccggtgta catggatgag taaacgccac gggtaaaggg gtactgtcca ggttcgccaa
      901 gagctacctc gggatcccaa tccccttggt ctgttgaacg ataaaccggc tttaccggaa
      961 accctgattc cgaaataccc atgatgtgag gttactgaca aaaagggatc tggcgaagcc
     1021 aaggacccca tatcggcact aatctgtagg taatccaaca gtttcaccga aaggtcactt
     1081 agtggcgtca acaaacggaa ccctctatcg cggtcgtgag ggtatgtggt cttgggtagc
     1141 acatcgcatt actggtgttg ctgtcttttt ctttttattc gtgcacgtat tagatactgc
     1201 attagttcgc gtttcacccg atgcatacga ccgggttatt gatacttaca aaacaccaat
     1261 tgtgaattta cttgaagttg gtttggtcgg tgcagtttta tttcatgcac taaacggaat
     1321 tcggctaatt ctggttgatt tttggagtaa aggtactaag taccatcgtc aaatgctttg
     1381 ggtcatagtt ggcatatggg ttgcagtgat gattccaggt acttacttca tgatgattca
     1441 cactattgaa gcaatgtttg ggaagtagtc atgagtacaa aaatgcctga agcattatta
     1501 gcaccacgat caagtagaag tggtcgcact aactatgaac tttattcgtg gctatttatg
     1561 cgcatttccg gtgtagtact ggtatttctt gtacttggtc accttttcat tatgaacatt
     1621 cttgatggtg gcgtgcaacg aattaatttc gcttttgtcg cgggccgttg gtcaagtccg
     1681 ttctggcaag tttgggattt aacacaactt tggctagcgc tactacatgg cggaaatgga
     1741 ttgcgaacca ttatcaatga ctacgccgaa cgggataata ctcgggtagt tcttaagtcc
     1801 ttactttatg tttctacagt atttattttg atgctgggca ctttagttat tttcaccttt
     1861 gatccaaata tcggctaagg ccaaggagtt aaatgcaaac gcataagtac gacgtagtaa
     1921 ttgtcggcgc tggtggcgcg ggcatgcgtg ctgcacttga atcaagcacc agggctagaa
     1981 ctgccgtatt aacaaagctt tatccgactc gttcgcatac tggtgctgca caaggtggta
     2041 tgtgtgctgc tttagctaac gtcgaagaag ataactggga atggcatacc tttgacactg
     2101 ttaaaggtgg tgactattta gtagatcaag atgccgctga agtcatgtgt aaagaagcaa
     2161 ttgacgcggt gattgattta gaaaaaatgg gtttgccttt taaccgcaca cctgagggca
     2221 aaatcgatca acgtcgcttt ggcggtcaca ctagaaatca tggcgaagcc gccgtgcgca
     2281 gatcatgttt tgctgctgac cgcactggtc atatgatttt gcagactctt tatcaaaact
     2341 gtattaaaaa tgatgttgac tttttcaacg agttttatgt tttggacctg ctacttgctg
     2401 atgatgagca agggaatcca attacctcgg gtgttgttgc gtatgaattg gcaactggcg
     2461 aaatacatgt attccaagct aagagtgttg tattggccac gggtggcttt ggcaaagtat
     2521 ttaaaaccac ttcaaatgcg cacactttga ccggcgatgg aatgggaatt gtctggcgaa
     2581 aaggtttgcc tcttgaggac atggagttct accagttcca tccaactggc ttagccggat
     2641 taggcgttct attaactgaa ggtgctcgcg gcgaaggtgg aatcctgcgt aacgcatctg
     2701 gcgaacgttt catggaacgt tatgcgccaa caattaaaga cttagcacca cgagatatgg
     2761 tagctcgagc aatggtgcaa gaagttcgtg agggtcgagg agcgggacca aataaagatt
     2821 atgtttattt ggatctaact catttaccag ctgaacagat tgaagccaaa cttccagaca
     2881 ttactgagtt ttctcgaacc tacttaggtg ttgacccgat taaagaacgg gtacctgttt
     2941 ttcctactgc acactacgca atgggtggtg taccgacaaa tgttgaaact caggtattgc
     3001 gcaataacac tgatatcgtg cccgggctat ttgccgcggg agaagttgca tgtgtttcag
     3061 tacacggtgc aaatcgcttg ggtactaatt cacttttgga catcaacgtt tttggtcgac
     3121 gcgctggcat ttatgctgct gagcacgccc tcaacagcga ctttgttgaa ctgccagaga
     3181 atccatcggc tgttgtcgaa gcactggtca acaatcttaa ggaaagtgac ggcactgaac
     3241 gcatagcagc gatccgaaaa gaattacaag aaaccatgga tttaaatgct caggtttacc
     3301 gtaacgatga aacactgaac aaagcactat ctgatattca ggtattaaag gaacgttaca
     3361 agaatgttgg aattcacgat aaaggtaagc gttataacac tgacttactt gaagctattg
     3421 aactaggttt cctacttgaa ctagctgagg ttttagtggt gagtgcattg gctcgcaaag
     3481 aaacacgcgg tggtcacgca cgtgaagatt accagactcg cgatgatgag aaattcatga
     3541 aacacacaat ggcttaccct gaccctaaaa atggcgttcg cctagattac aaaccagtca
     3601 ctatgactag gtatgaaccg atggagcgta aatactaatg gcatatttgg cagatgaaat
     3661 aacggacttg gttggcacaa tgaatgcagt tgatccagga gttattaatg cttcggtttc
     3721 aacaaatagc gatgcaccaa agccattcac tgtcactgtg actgttcgcc gttttgatcc
     3781 agaagttgac caagaccctt tctggcaaga tttccaagtt gaagtatttt caaccgatcg
     3841 aatcttagat gcgctacata aaatcaaatg ggagcaagac ggtacgctaa gcttccgtcg
     3901 ctcatgtgcc cacggtattt gtggctcaga tgcaatgcgg attaacggac gcaaccgatt
     3961 ggcttgtaag acactgatta aagatctaga tgccactaag ccaataactg ttgaagcgat
     4021 taaagggcta ccactagaaa aagatttaat cgttgacatg gaacctttct ttgctgctta
     4081 tcgcgaagtt atgccgttcc tgattcctaa aggtcatgag ccatcacgcg aacgtgtcca
     4141 gagccaagaa gaacgtgctc gattcgatga cagtacaaaa tgtattttgt gtgctgcgtg
     4201 cacaagtagt tgcccagttt tctggaccga caatcagtat tttggtccag ccgcaattgt
     4261 tggtgcgcat cgatttattt ttgatagtcg cgatgccgca gctgaaactc gcttggaaat
     4321 tttaaatagt aaagaaggtg tctggcgttg ccgcacgacc tttaactgca ccgaagcctg
     4381 cccacgtgga atacaaatca ctcatgcaat tgctgaagtt aaacaagctt taattcgcgg
     4441 gaaaatttaa ctaaaaagta aattggctag ttggtcgacc gaatcaacca cgtctattgc
     4501 tccagctttt gctaactcgc cctcttcggc ataaccccag ctgactccaa ttgccggcag
     4561 cttatgttca gctgcgccat gaacgtcatg ttcgcgatcg ccaatcatta cgatagaggc
     4621 accgtcacac atactaagtt ctgctagtgc gtgctcaatt actaacgctt tcgtacctcg
     4681 gtgtactcca tgctcatcgc caccggctat cacatcaaag tggtgatcta aattaaagtg
     4741 tcgcaaaata tttaccgctg cataatcaac ttttgaagtt gcaacagcca agcgtcgctc
     4801 ttttttattc agctcagtta gtaactcagg aatcccggga taaacactat tttcataagc
     4861 accagtttcg ttgtagtact tgcgatacaa ctcaaccgcc aatttagttt gagcttcatc
     4921 catgccagca aagtctctaa aagaagtcca aagaggtgga cctaaatatt tacgtaactc
     4981 cccattctct ggttcaggaa atcccatttc gcgtattgcg taaagtacac cgttacctac
     5041 gccaaggcca gaatcagtta atgttccatc taaatcaaat agaacgacat cccagctcat
     5101 tttttactca ccgggcgaag ccgacttttt catacactgt agtcaatgtt ttctcggcaa
     5161 tctgttgagc ttttgccgca cccgcatgca ttaatttttc tagttctgct ttttctgtta
     5221 acaattcttg aactcgcgcc tggtatggct caataaatga aatgacgact tgcgcagttt
     5281 cttgctttaa atcaccatac atgcgtccag aaaagtcatt aactaattgt gaaacggact
     5341 tacccgaata ggcactatta attaggagca aattagagac tcctggtttt ttagtttgat
     5401 caaacttaat ttctgtttct gaatcagtaa ctgcagattt aaatttcttt tcagtggctt
     5461 taatcggatc taataaatta atgcagccgc caggtgatga tttactcatc tttgccgttg
     5521 gttcctgtaa atccattatc ttggcagttt ctttaacaat gaaaggctcg ggaacagtaa
     5581 atacttcacc gaaacgatta ttgaatctca gtgctagatc tcgggtcagt tctaggtgtt
     5641 gccgctgatc ttcaccaacc ggcacttgat tggcttggta aagcaggatg tcagctgcct
     5701 gcaaaattgg gtaagtcagt aacccaacac tcgaggatgc cgcgccacct tttgctgatt
     5761 tatctttgaa ctgagtcatg cga
//
LOCUS       DBNHVN010000155         1880 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_2035905, whole genome shotgun sequence.
ACCESSION   DBNHVN010000155 DBNHVN010000000
VERSION     DBNHVN010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_2035905"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..>1880
                     /gene="glgX"
                     /locus_tag="AC16Y1_02995"
     CDS             118..>1880
                     /gene="glgX"
                     /locus_tag="AC16Y1_02995"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120549 - limit dextrin
                     alpha-1,6-maltotetraose-hydrolase activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HIR5831463.1"
                     /translation="MHGIQAGLPEPLGATLVSGGVNFAVWAPESDRVELCLLDDDGAH
                     WNIDLPGFTKGVYHGFIPDIGAGQRYGFRVHGTWQPENGLRFNPAKLLIDPYAKAITG
                     ELKFHEAIFGHIGNDAQVFNDMDSSPFVPNSVVVDDSYDWGDDKSPRIPLANTIIYEA
                     HVVSTTKTHPEVPENLRGTYAGLANPAIISHLKNLGVTTIELLPVHHWVSETFLLARG
                     AHNYWGYNSIGFFAPHAGYSSSGTRGEQIREFKDMIKTFHQSGLEVILDVVYNHTAEG
                     NEFGPTLSFRGLDNSGYYHLAEDQSKYWDSTGCGNTVRASHPQTLRLIMDSLRYWVTE
                     MHVDGFRFDLTSALTRNSANEVDLEGAFLTAVTQDPILRNVKLIAEPWDLGNGGYQVG
                     SYPAPWSEWNDQFRDTVRDFWRGQSGGVAELGWRLTGSADMYWDQVGGSHASVNFVTA
                     HDGFTLRDLVSYNEKHNLANGEENQDGNNSNRSWNCGVEGETQDATINSHRQRQMRNL
                     LATTILSAGIPMLLAGDETGRTQQGNNNAYSQNNEISWLNWNFAPWQNDLREFTTHLI
                     KIRQAHRVFQRSGYLKGENVA"
BASE COUNT          549 a          395 c          434 g          502 t
ORIGIN      
        1 aaattcatac atataaataa gcctaaccat ttagttatgt cgccacctat tctgcaagtt
       61 ttttaggcaa gatagtgttt tgtcagtaaa aagtttgatc aacaggatgg ggcggtaatg
      121 cacggtattc aagccggctt gcctgaacca ttgggtgcaa ctttagtttc cggtggcgtt
      181 aattttgccg tgtgggctcc agaatcagat cgagtcgaac tctgcttact tgacgatgac
      241 ggtgcacatt ggaatataga tctgcctggt tttactaaag gggtttatca cggattcatc
      301 cccgatattg gtgcaggcca gcgttacggt ttccgagtgc atggcacctg gcagcccgaa
      361 aatggattac gttttaatcc agcaaaatta cttattgacc cctacgccaa agcaataacg
      421 ggcgaactta aattccacga agcaattttc ggacatatcg gcaatgacgc ccaagtattt
      481 aacgacatgg atagcagtcc atttgtcccg aactcagttg tagttgatga ctcatacgac
      541 tggggcgatg acaaatcgcc gcgtattccg ctggcgaaca caattattta tgaagcacac
      601 gtagtctcaa caacaaaaac tcacccagaa gttccagaaa atctacgtgg gacctatgca
      661 gggttagcta atccagcgat tatttctcac ctaaagaatt tgggtgtcac cacgattgaa
      721 ctactgccag tacatcactg ggtcagtgaa acttttctat tggcaagagg tgcgcataac
      781 tattggggat acaactcaat tggatttttt gctccacatg ctgggtactc atcttcgggc
      841 actagaggtg agcagatccg cgaattcaaa gacatgatta aaacgttcca tcaatctgga
      901 ctcgaagtaa tccttgatgt cgtctacaac cacacggctg aaggaaatga atttggcccg
      961 acactttcat tccgtggctt agacaattct ggctactacc atctcgctga agatcaatca
     1021 aagtactggg attcaactgg ttgcggaaat actgttcggg caagtcatcc gcaaactctt
     1081 cgactcatta tggactcatt acgatattgg gtaactgaaa tgcatgttga tggtttccgt
     1141 tttgatttaa caagtgcact gactcgaaat tctgcaaatg aagttgattt agaaggtgct
     1201 tttctgactg cggtaacaca ggatccgatt ttgcgaaatg tgaagttgat tgctgaaccg
     1261 tgggatcttg gaaatggtgg gtaccaagtt gggtcctacc ccgcaccatg gagtgaatgg
     1321 aatgatcaat ttcgcgacac agttcgtgat ttttggcgtg gacaaagtgg cggtgtcgca
     1381 gagcttggct ggagattaac tggttcagca gatatgtatt gggatcaagt aggtgggtcc
     1441 catgcttcag tgaactttgt aaccgcgcat gatggcttca ccttgagaga cttagttagc
     1501 tataacgaaa aacataacct ggctaatggt gaggaaaatc aagacggtaa caattcaaat
     1561 cgatcatgga attgtggtgt cgaaggagaa actcaagatg caacaatcaa ctcacatcga
     1621 caacgccaaa tgcgaaattt acttgccacc acaattttga gtgctggaat tccaatgttg
     1681 ttagctggag atgaaactgg cagaacccaa caaggaaaca acaatgccta cagtcaaaac
     1741 aatgaaatat cctggctaaa ttggaacttt gcaccttggc aaaatgactt acgagaattc
     1801 actacacact taattaaaat tcggcaggcc caccgagtat ttcagcgaag tggttaccta
     1861 aaaggtgaaa acgtggcgta
//
LOCUS       DBNHVN010000156         1860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1887389, whole genome shotgun sequence.
ACCESSION   DBNHVN010000156 DBNHVN010000000
VERSION     DBNHVN010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1887389"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="AC16Y1_03000"
     CDS             <1..694
                     /locus_tag="AC16Y1_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5831464.1"
                     /translation="VDDQSTYPAEAPITDLSGFTPNVEAIVAKQPDLVVVSYDADNLI
                     ASLQAANIPVLLQNAAVDLDGTYGQITELGLATGQVQGANDLVTQMKDDISAALASIK
                     PLAEPLTYYHELDPTFYTVTSSTFIGQLYALAGLENIADAAPDAESGYPQLSAEYIVQ
                     ANPDLIFLADTKCCAVTQAELATRAGFNSLTAVKSGGVIELDDDVASRWGPRTTELFA
                     AIVKAVNDLAAS"
     gene            709..1752
                     /locus_tag="AC16Y1_03005"
     CDS             709..1752
                     /locus_tag="AC16Y1_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017837302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR5831465.1"
                     /translation="MSTTATIKKLPSLTWLFAMFAVVLALLIGLGVGAVSIPATDALQ
                     EILSRMTNGLIKSPLSETQATIIWDLRFPRVLLALLVGAMLASAGCAYQGSFRNPLAD
                     PYLLGVSAGAGLGATIVIFTQNQTTRWELPLAAFIGALGAVMLTYLLGRSAIVGRSST
                     SLVLAGVAVASMLTAIQTLLQQRNTDKIREVYSWVLGRLSTAGWGDVILVTPYILICI
                     TILFLYRRILDVFSVGDEEAHTMGLSVGRARAAIIIAASLGTAAAVSVTGLIGFVGII
                     VPHILRLIVGHSYRNLMPLATIYGGVFLVLADVVARTALAPQEISIGVITALIGAPFF
                     LFILGSSKHGSIT"
BASE COUNT          464 a          401 c          427 g          568 t
ORIGIN      
        1 ggttgatgac cagtcaacat atcctgctga agctccaatt acagatctat ctggatttac
       61 cccaaatgtt gaagcgattg ttgctaagca gccagattta gttgtagtca gctacgacgc
      121 tgacaactta attgcttcat tacaagcagc aaatattcca gtacttctgc aaaatgccgc
      181 agttgattta gatggaactt acggtcaaat aactgaactc ggattggcta ctggccaggt
      241 gcaaggtgcc aatgatcttg taacccagat gaaggatgac atttctgccg cattggcttc
      301 aatcaagcca cttgcagagc cgttgactta ctaccatgaa ttagatccga cgttctatac
      361 cgttacttct tcaacattca ttggtcagtt atacgcgcta gctggactgg aaaacatcgc
      421 tgacgctgca ccagatgccg aaagtggtta tccgcaactt tcagctgagt acattgtgca
      481 agcaaatcca gatttgattt tcttggcgga cacaaaatgc tgtgcagtaa ctcaagctga
      541 acttgcaaca cgtgccggct ttaattcact aactgcggtt aaaagtggcg gcgttattga
      601 attagatgac gacgttgcaa gtcgctgggg tccacggaca actgagttgt ttgctgcaat
      661 cgtaaaagca gttaacgatt tagctgcctc ttaatcagag ataagtacat gagcacaact
      721 gcgaccatta aaaaattacc aagtcttact tggttgtttg caatgttcgc agttgtgctc
      781 gctttattga ttggcttggg cgttggtgca gtttcaattc ccgcaactga cgctttgcaa
      841 gaaatacttt cccgcatgac taacggttta attaaaagcc cgctatcgga aactcaagcg
      901 acgatcatct gggacttgcg attcccacgg gtgcttcttg ccctcttggt tggcgcaatg
      961 ctagcgagcg caggatgtgc ttatcagggc tcatttagaa atccgctggc ggatccttac
     1021 ctacttggtg tttcagcggg tgcaggcctt ggtgcaacca ttgtaatttt tactcagaat
     1081 caaacaacaa ggtgggaact tccactagca gcatttatcg gcgcacttgg tgctgtcatg
     1141 ctcacttatt tattaggtcg ttcagcgatt gttggaaggt cttcgacttc tttggtgctt
     1201 gctggtgttg ccgtggcaag tatgttgact gcaatccaaa ctttgttgca acaacgaaat
     1261 accgacaaaa ttcgtgaagt ttattcctgg gttcttggcc ggttatcaac agccggatgg
     1321 ggtgacgtga ttttagttac cccgtacatc cttatttgca tcacaatttt atttttatac
     1381 cgacgtattt tggatgtgtt ttcggttggc gatgaagaag cacatacgat gggactttca
     1441 gtcggacgtg ctcgagccgc gataataatt gccgcttcac ttggaactgc tgctgcggtc
     1501 tcagtaaccg gacttattgg ttttgttgga attattgttc cacacatatt gcggttaatt
     1561 gtgggtcata gctatcgaaa tctaatgcca cttgcaacta tttatggcgg tgtcttctta
     1621 gtccttgcag atgttgtcgc gagaactgca cttgccccac aagaaatatc tatcggcgtt
     1681 ataactgctt tgattggcgc accatttttc ttgttcatcc ttggttcaag taaacatggg
     1741 tcgataacat gaccaaatta aatgtgagtg gtctaaccgt taagttagcc aataaaactg
     1801 tcgtagataa tttttcaatt tcactttccg ttggtgattg ggtttgtgag attggtccaa
//
LOCUS       DBNHVN010000157         3405 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1219372, whole genome shotgun sequence.
ACCESSION   DBNHVN010000157 DBNHVN010000000
VERSION     DBNHVN010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1219372"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="AC16Y1_03010"
     CDS             complement(<1..119)
                     /locus_tag="AC16Y1_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011692322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR5831466.1"
                     /translation="MKIDAVTVFPEYLAPLQLSLIGKAIEDGVIELAIHDLRD"
     gene            complement(116..646)
                     /gene="rimM"
                     /locus_tag="AC16Y1_03015"
     CDS             complement(116..646)
                     /gene="rimM"
                     /locus_tag="AC16Y1_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010061964.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR5831467.1"
                     /translation="MRLVVGRIGRAHGVRGDLFVEPLTDEPENRFAIGEKLQTEKNGL
                     LTVADQKWHSGKLILHFSGVDDRNSAELLKGCELVIEVDPNVLPDDPDEFYDHQLVGL
                     KVVFNEEVIGQVSEVIHLPAQDLLSVSQIDGIEVLIPFVKQIVPEVDLRKGQLVITPP
                     PGLLDDSHAIEAGESE"
     gene            complement(651..884)
                     /locus_tag="AC16Y1_03020"
     CDS             complement(651..884)
                     /locus_tag="AC16Y1_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003781480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HIR5831468.1"
                     /translation="MLDEALEHLVKGIVDHPEDVVVREKNRKGPTLEVQVNPEDMGRV
                     IGKAGRTASALRTVVNALSDKNVRIDILDVDER"
     gene            complement(888..1457)
                     /gene="rpsP"
                     /locus_tag="AC16Y1_03025"
     CDS             complement(888..1457)
                     /gene="rpsP"
                     /locus_tag="AC16Y1_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007323040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR5831469.1"
                     /translation="MAVKIKLKRLGKIRNPQYRVVIANSRTARDGRSIEEIGLYHPKE
                     EPSLIQINSERVQYWLGVGAQPTEPVLALLKITGDWQKFKGLPGSEGTLKVAPPKVDK
                     NTVFAQTVQAVANEPRTPTIRPKVEKPAPEVKAEEVKAEEAVTAQPAEATSEEVVQTA
                     AEVADESGAAAPADTTSADEVSAEVVEEA"
     gene            1666..2037
                     /locus_tag="AC16Y1_03030"
     CDS             1666..2037
                     /locus_tag="AC16Y1_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831470.1"
                     /translation="MYPKVDVSNNQVLINFSIIDQFINAKPGGIKFRLDQVESIDAGA
                     HIFKQLRGLRAPGTYAFVLIGGTYWRSRKTREFWNARRRFADQTVRINLRNHFYRSIV
                     IQVPDVELFKKKLNLAATENL"
     gene            complement(2021..>3405)
                     /gene="ffh"
                     /locus_tag="AC16Y1_03035"
     CDS             complement(2021..>3405)
                     /gene="ffh"
                     /locus_tag="AC16Y1_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR5831471.1"
                     /translation="LPVVRDFCAKVKERALDSEVSAALNPAAQIIKIVNEELTNILGG
                     QTRRIKLAKNPPTVILLTGLQGAGKTTLAGKLALHLRSLGHQPLLVAADLQRPNAVDQ
                     LKIVGEQAQTPVFAPEAGNGVGDPVKVSREAIKFAAQKLHDIVIVDTAGRLAIDAQLM
                     DQLRKIKKEVQPTETLLVIDAMTGQDAVNTASTFAQEVGLDAVVLTKLDGDARGGAAL
                     SVTTVTNTPIMFASTGEKLTDFEVFHPDRMASRILDMGDVLTLIEQAEKTFDQDQTAK
                     LAKKVQDGDDFTLTDFMEQMKQLKKMGNFSKMLGMLPGAGEMKKQLENFDEKELDRVG
                     AIISSMTVTEREDPKILNGSRRARIAKGSGTQVSDVNNLIERFTNAAKMMKQIAKGGV
                     PNMPGLPNMGGLGLPKPPAQKKVQKPGKGAKKSGNPAKRAAQEAGLDVVNQKPADKLT
                     ALPNAFKDFL"
BASE COUNT          853 a          799 c          693 g         1060 t
ORIGIN      
        1 taatctcgta aatcatggat cgccagttcg atgaccccat cttcgatcgc tttgccaatt
       61 agcgaaagtt ggagtggagc taaatattct gggaaaacgg tgacagcatc tatcttcatt
      121 cgctttcacc ggcttcaatt gcatgtgaat catccaagag tccagggggc ggagtaatta
      181 ccaattgccc cttgcgtaag tccacttcag gaacgatctg cttaacaaac ggaattaaaa
      241 cttcgattcc atctatctgg gaaacagata ataaatcctg cgcaggtagg tggatcactt
      301 cagaaacctg tccaataact tcttcgttga aaactacttt caggccaaca agttgatgat
      361 cataaaactc atctggatca tcgggaagca catttgggtc aacttcgatt accagctcgc
      421 agcccttaag tagttctgcc gaatttcgat catcgactcc actgaaatgc agaatcaatt
      481 tgccactatg ccacttttga tcagcaacag taagcagccc atttttttca gtctgcaatt
      541 tctcgccgat agcaaagcga ttttccggtt cgtcagttaa cggttcgaca aaaagatccc
      601 cacgcacacc gtgcgctcga cctattcgcc cgacaacgag ccgcatttat ttagcgttcg
      661 tctacatcaa ggatgtcgat gcgcacattc ttatctgaaa gtgcgtttac cactgtgcga
      721 agtgcgcttg cagtgcgtcc agcttttccg atcactcgac ccatgtcttc aggattaacc
      781 tgaacttcaa gggtcggtcc tttgcgattt ttttcgcgca caactacatc ttcgggatga
      841 tcgacaatgc cctttacgag atgttctaat gcctcgtcca gcatggatta agcctcttcg
      901 actacttctg cactgacttc atctgctgaa gttgtatcag caggagccgc tgcacctgat
      961 tcatctgcaa cttctgctgc agtttggaca acctcttcgc tagttgcttc agctggttgc
     1021 gcagttactg cttcttcagc cttaacctct tctgctttta cttctggagc aggcttttca
     1081 acttttggtc gaatagttgg tgtgcgaggt tcatttgcca cagcttgaac tgtttgggcg
     1141 aacacagtgt tcttatccac tttaggtggc gcaactttaa gagtgccttc acttcctgga
     1201 agccccttaa atttttgcca gtcaccagtg atctttagca atgcaagtac aggttcagtt
     1261 ggttgtgcgc caacacctaa ccagtattga acacgttcag aattgatctg aattaagctc
     1321 ggctcttcct ttgggtggta caaacctatt tcttcgatgg aacgtccatc acgggcagta
     1381 cgtgagttag caattactac gcgatattgc ggattacgaa ttttgccaag tcgctttaac
     1441 ttgatcttta cagccacgtt ttttatatct cctgttaatt gggtgatcac aacttttaaa
     1501 gctttagtgg ggtaaaactt ttacttggcg atcgatgaat ccaaaccttt agcggttaga
     1561 gggccggcta gaagttcggg attacttgca atagtttgcc agatcccttt gatttgggga
     1621 aattggctgc tcgaagccca atttatcagc gtttaaacta tcaatatgta cccaaaagta
     1681 gatgtttcca ataatcaggt tttgattaac ttctcgataa ttgaccaatt tattaacgcg
     1741 aagccaggtg gaatcaagtt tcgccttgat caagtggaaa gtattgatgc aggagcacat
     1801 atttttaagc agttacgcgg tttacgtgca ccaggcactt atgcgtttgt cctaattggc
     1861 ggcacatact ggcgaagtcg gaagactcgt gagttttgga atgcgcgacg tagattcgcc
     1921 gaccaaactg ttcgaattaa tttacgcaat catttttacc gctcaattgt tatccaagtg
     1981 cctgatgttg aattattcaa aaagaaattg aacttagctg ctacagaaaa tctttaaatg
     2041 cgttaggtaa cgcggttagt ttgtcagcag gcttttgatt gactacatct aatccggctt
     2101 cttgcgccgc tcttttggca gggttacctg atttctttgc acccttacca ggtttttgca
     2161 cttttttttg tgcaggtggt ttcggtaaac ctagtccacc catgttgggc agcccaggca
     2221 tattcggcac tccacctttt gcgatttgtt tcatcatttt tgcggcatta gtaaagcgtt
     2281 caattaaatt atttacatcg ctaacttgag taccgctacc ttttgcaatg cgtgctcgcc
     2341 ttgagccatt aagtattttt ggatcttccc tttcggtaac ggtcatcgaa gaaattatgg
     2401 caccaactcg atctaattct ttttcatcaa agttctctag ttgttttttc atttcaccag
     2461 cacccggcaa catgcctagc attttgctga aattgcccat cttctttaat tgcttcattt
     2521 gttccatgaa gtcagtgagc gtgaaatcat caccatcctg aactttttta gctaacttcg
     2581 ccgtttgatc ttggtcaaaa gttttttcag cttgttcaat caaagtaagc acatcgccca
     2641 tatctaaaat gcgcgaggcc attcgatctg gatgaaaaac ttcaaagtca gttagttttt
     2701 cgccagtcga agcaaacatt attggtgtgt ttgtcaccgt ggtcacagat agcgcagcac
     2761 cgccgcgagc atctccatcg agtttagtta gaacgacagc atctaagcca acttcttgcg
     2821 caaaagttga cgcagtatta accgcatctt gtccagtcat tgcatcaatt actaataaag
     2881 tttcggttgg ctgaacctct tttttgattt ttcgaagttg atccatcaac tgcgcatcaa
     2941 tagctaaccg gccagcagta tcgacaatca caatgtcatg taacttttgc gcagcaaact
     3001 taatcgcctc gcggcttact tttactggat cgccaactcc attaccggct tcaggtgcaa
     3061 aaactggagt ctgcgcttgc tcacctacaa tttttaattg atcaactgca tttggacgct
     3121 gcaaatcagc tgctaccaac agcggctgat ggccaaggct tcgaaggtga agagctaact
     3181 tgccagctaa tgttgtttta cccgcaccct gtaaacccgt taatagaatt accgttggcg
     3241 gatttttagc taatttaatc cgtcgagttt ggccacctaa tatatttgtt aactcttcgt
     3301 ttactatctt gataatttgt gctgctggat ttaaggcagc agatacttca ctatctaaag
     3361 ctcgctcttt aacttttgca caaaagtcac gtacgacagg aagtg
//
LOCUS       DBNHVN010000158         2919 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_2036145, whole genome shotgun sequence.
ACCESSION   DBNHVN010000158 DBNHVN010000000
VERSION     DBNHVN010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_2036145"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="AC16Y1_03040"
     CDS             complement(<1..1055)
                     /locus_tag="AC16Y1_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR5831472.1"
                     /translation="MTTNKVNPQRKTISEHLAHPYAHYNLLLGATLILLIFGLVMVTS
                     ASSIYSYQINDGNSWAIALRQAIYAVAGVGVMIWIQRVPLVNVRRMIPFFMIFVLVLL
                     VLVLIIGTAKHGQRNWIEFGSFARIQPSEFAKLAVVLFAADMLERKSHLLNYRSELMR
                     PVGWLFLIILGLILLEGDVGTSIVVVPIMLAAFYLIGAPMRIFVAIAALGIAGIAALT
                     AIAPYRMARFTSWLNPQADPQGAGFQLIHGQQALGSGGLWGRGLGGSREKWGTLPEAH
                     TDFIFAVIGEELGIFGTLSVLFLFFLIAFVGLRIARKTDDLFIELASFGIVVWIMWQM
                     MVNIGAVLRIMPITGVP"
     gene            complement(1045..2493)
                     /gene="murD"
                     /locus_tag="AC16Y1_03045"
     CDS             complement(1045..2493)
                     /gene="murD"
                     /locus_tag="AC16Y1_03045"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR5831473.1"
                     /translation="MNSFDVQTLTRESDWSQLRVCVVGLGIAGFACADALIQMNAQVV
                     VLDANPGEKQKERATLLEILGAKIIFGDDNTLPKNIDLLVLSPGVSPLAPIVSAANEY
                     KVPIWGEIELAWHLRDLTHPAPWLLITGTNGKTTTTLMLESILRAAGKRTVAAGNIGR
                     SLVEVVMDPQPWDVLAIEIGAPQMPFVHTVTPQAAVCLNLAPDHLDLFGTFEAYRNAK
                     ARIYQRCEVAAIFNVADPQTISMVEEAEVTEGCRAIGFTLGIPDVSMLGVVDEFLVDR
                     AFLSERKESALELAEVTDLAINAKHNIENALAASALARAHGIEPRFIRDGLRAWQPAA
                     HRIAHVATINGVTYIDDSKATNTHAAMTSLTAYPKVVWIAGGMAKGQDFDELVLATAN
                     RITGVVLLGVDQEQIAAALAKHAPQIPITKISNTDTSAMELVVAAAAKYAQEGDTVLL
                     APGCASWDMFKDYSHRGQVFAQAVQKLADNDN"
     gene            complement(2503..>2919)
                     /gene="mraY"
                     /locus_tag="AC16Y1_03050"
     CDS             complement(2503..>2919)
                     /gene="mraY"
                     /locus_tag="AC16Y1_03050"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019545784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR5831474.1"
                     /translation="IPGCYTVRDPLDLAIFAAAVGGACFGFLWWNSSPAKIFMGDTGS
                     LALGGAIAALAIMTRTELLLALIGGLFVIVTLSVIAQVGSFKLTGKRVLRMAPLHHHF
                     EMLGWPEVQIVVRFWIIQGLFVIIGMALFYAEWIRA"
BASE COUNT          865 a          700 c          618 g          736 t
ORIGIN      
        1 aatggcacac cagtgattgg cataattcga agtacggcac caatgttcac catcatttgc
       61 cacataatcc aaaccacgat gccaaacgag gcaagctcaa taaataggtc atctgttttt
      121 cgggcaatac gtaagccgac aaaggcaatt aagaaaaaca ggaataacac actgagtgtg
      181 ccaaagatgc caagctcttc gcctattacc gcgaagataa agtcagtatg agcctcggga
      241 agcgtgcccc atttttcgcg tgacccacct aacccgcgac cccaaagtcc acctgagcca
      301 agtgcttgtt gaccgtggat taattggaaa cctgctcctt gtggatctgc ttgcggattt
      361 aaccacgaag tgaatcgcgc catgcgataa ggcgcaattg cagttaacgc tgcgatgcca
      421 gcgataccta aagccgcaat ggccacaaat attcgcatcg gtgcgccaat taaataaaaa
      481 gctgccaaca taattggcac tacaacgatt gaagtaccga catcgccttc aagcaaaatt
      541 aagcccaaga taattaaaaa taaccaaccg actggacgca ttaactcact tcgatagtta
      601 agtaagtgtg acttgcgttc cagcatgtcg gcagcgaata acactactgc taactttgca
      661 aactcagaag gttgaatacg agcaaaagat ccaaactcaa tccagttacg ttgtccgtgc
      721 tttgccgtac caataatcaa aaccaaaaca agtagcacaa gcacaaaaat cataaaaaat
      781 ggaatcatcc gtcgaacgtt tacaagtggc actcgttgaa tccaaatcat tacgccaacg
      841 ccagccactg catatattgc ttggcgaagt gcgattgccc aagagttacc atcgttaatt
      901 tgataagaat aaatacttga tgctgaagtc accataacta gaccaaaaat cagcaaaatt
      961 aaggttgcgc caagtaacaa gttgtagtga gcatatgggt gagccagatg ttcgctaatt
     1021 gttttacgtt gtggatttac tttattagtt gtcattgtca gccaacttct gaacggcttg
     1081 ggcaaaaact tgtccgcgat gtgagtaatc cttgaacata tcccaagacg cgcaacctgg
     1141 ggcaagtagt actgtgtcgc cttcttgggc atactttgcc gcagcagcta caactaactc
     1201 catagctgaa gtatctgtgt tggatatctt ggttattggg atttgcggtg cgtgttttgc
     1261 caacgcagcg gcaatttgtt cttggtcaac gccaagaagt actacacctg tgatccgatt
     1321 tgctgtggcc agcaccaatt cgtcgaagtc ttggccctta gccatgccac cggctatcca
     1381 aactactttt ggataggccg ttaacgacgt catggccgca tgggtattag tcgcttttga
     1441 atcatctatg taagtaaccc catttattgt ggctacgtgc gcaattcgat gggcagctgg
     1501 ttgccaggcg cgcaagccat cgcggataaa gcgcggctca atcccatgtg cccgggccag
     1561 ggctgatgca gccagcgcat tttcgatatt gtgttttgca ttaatcgcta aatcagttac
     1621 ttcagctaat tccaaggccg actctttgcg ttcggataaa aatgctcggt cgactaggaa
     1681 ttcatccaca acgccaagca ttgatacatc tgggatacca agagtgaagc caatggcccg
     1741 acaaccttca gttacttccg cctcttccac cattgaaata gtttgtggat cagccacgtt
     1801 aaaaatagct gctacttcac agcgctgata aattcgggct tttgcatttc gataagcctc
     1861 aaatgtgcca aataaatcta aatggtctgg tgctaagttt aagcaaactg cagcctgtgg
     1921 tgtaactgta tggacaaatg gcatctgcgg agcgccgatt tcaatggcca acacatccca
     1981 aggttgtggg tccatcacta cttcaactag tgagcgtcca atgtttccgg ctgcgactgt
     2041 acgctttccg gcagcgcgca aaattgattc caacattaaa gttgtggtgg ttttgccgtt
     2101 agttccagta attaaaagcc aaggcgcagg atgagttaaa tcgcgcaagt gccaagctaa
     2161 ttcgatttca ccccatattg gaactttata ctcatttgct gcactgacaa tcggagcaag
     2221 tggtgagact cctggcgata gaaccaacaa atcaatattc tttggcaaag tattgtcatc
     2281 accaaaaata attttggcgc caagtatctc aagtaaggta gcgcgctctt tttgtttttc
     2341 acctggatta gcatctaaga ccacaacttg ggcattcatt tgaattaatg catcagcgca
     2401 agcaaatccg gcaataccta aaccaactac acaaactcga agttgtgacc agtcagattc
     2461 acgagtgaga gtttggacat cgaaactatt catggcttcg tcctacgccc gaatccattc
     2521 ggcataaaaa agtgccatgc cgatgatcac aaataaacct tgaatgatcc aaaatcgcac
     2581 aactatttga acttctggcc agcccaacat ttcaaaatga tgatgtagtg gggccattcg
     2641 caatactcgt ttaccagtta atttaaatga acctacttgg gcgataaccg aaagtgtgac
     2701 aatgacaaat aacccgccaa ttaaagcaag taataattcg gttcgtgtca taattgctag
     2761 cgctgcaatt gcgccaccaa gtgcaagcga tccagtgtct cccataaata tttttgctgg
     2821 acttgaattc caccagagaa aaccaaagca agcaccgccc actgctgccg caaaaattgc
     2881 taagtcaagt ggatcgcgaa ctgtataaca accaggaat
//
LOCUS       DBNHVN010000159         5216 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1219571, whole genome shotgun sequence.
ACCESSION   DBNHVN010000159 DBNHVN010000000
VERSION     DBNHVN010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5216)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5216)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5216
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1219571"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="AC16Y1_03055"
     CDS             complement(<1..440)
                     /locus_tag="AC16Y1_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005203695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HIR5831475.1"
                     /translation="MKIGNSCSKNSVELLMSTGLMPPLVIDSFQKAENLLVEQAKFRV
                     NQKRVGSLSMTKSPATDDDFSSAQLNADEIDSVAAKKLSPAKAAELTARFERDALPLL
                     DAIYAGAFRLTRNQADAEDLVQETFAKAYRAFQSFEEGTNLKAWL"
     gene            478..1755
                     /gene="aroA"
                     /locus_tag="AC16Y1_03060"
     CDS             478..1755
                     /gene="aroA"
                     /locus_tag="AC16Y1_03060"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014910848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR5831476.1"
                     /translation="MSELWIAPNRDSTISKTVQIPGSKSLTNRWLVLAAIADGPSRIN
                     LPLKARDTELMANAIQSLGNSIEVDGQAWVVTPGKFSGSTEIDCGLAGTVMRFVPPIA
                     ALASGAVRFDGDPRARVRPMSGVINALRALGADITDDGRGTLPFVINAKGFLPGGQVT
                     IDASASSQFVSALLLAGARFDDGLTVTHSGGKLPSLPHIEMTVEVLARAGVKVAADLI
                     DLQNATWTIAPQIPQGFSITVEPDLSNAAAFVAAALVAGGEVTIENWPEQTTQAGNAL
                     IDLIPMLGGQVSRNGNNLVFSGTGAINGISADLSAVGELTPVIAAMCALADTPSELTG
                     IGHLRGHETDRLAALATEINKMGGQVTQMQDGLQINPSSLSGGVFNTYEDHRMAMAGA
                     VLGLKVKNLQIENISTTSKTLPEFAQMWLELVS"
     gene            1755..2783
                     /gene="rsgA"
                     /locus_tag="AC16Y1_03065"
     CDS             1755..2783
                     /gene="rsgA"
                     /locus_tag="AC16Y1_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008362049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR5831477.1"
                     /translation="MVNYDEDKFDRPSRFNSRRRSKDRPDFSTAQVGQIIGIDRGRIT
                     CVYPIATNHEEQTRVTAVKARELGRKGVTIGDQVRLVGDLSGKADTLARLVEVLPRET
                     ELMRTADDSDPVERVIVANADQIAIVTSIANPEPRLGLIDRSLVAAFHSGLKPLLVIT
                     KTDLANPAQLLQNYEGIDLPFILVNNKTGEGLNKLLAEIKGHNTVFIGHSGVGKSTLV
                     NALVPDAQRITGDVNDVTGRGKHTSTSAIALPLPNDTGWVIDTPGVRSFGLAHVKPED
                     IIESFPELIDGTLKCPRACTHVNSVGQRPPDCALDDWVSDGNAGAAGLARLNSLRRLL
                     FALESPSS"
     gene            complement(2742..3143)
                     /locus_tag="AC16Y1_03070"
     CDS             complement(2742..3143)
                     /locus_tag="AC16Y1_03070"
                     /inference="COORDINATES: protein motif:HMM:NF018508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HIR5831478.1"
                     /translation="MFGRKKYSKKAVRETVKATTDFFSSNPDKIQAVLDAFPKKLTAA
                     GKIPFAEQITAAYYALRDSKTPFKVKAILAAALAYFIMPADLIPDLVAGFGFTDDFAV
                     LLLVLKRVSGSITKEHFELARRRLKGEQESA"
     gene            3200..3967
                     /gene="hisN"
                     /locus_tag="AC16Y1_03075"
     CDS             3200..3967
                     /gene="hisN"
                     /locus_tag="AC16Y1_03075"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017608283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HIR5831479.1"
                     /translation="MFDLEFAQTLADVADKITLGRYLAQDLKIETKPDATPVTEADHA
                     TELKLRELIQSTLPADGILGEEFGQTDGSNNRVWILDPIDGTKNYLRGVPVWATLIAL
                     VEDDEPIVGMVSCPALGRRWWASKNNGAFTSDFDGTIRKISVSGVSKINDAFFSYSDR
                     IGWEKFSGAFQKLTDSVWRTRAYGDFLSHLLVAEGAVDIAAEPELKKWDMAANNIIVT
                     EAGGLVTGLKGGRAFDEFNALSTNGKLHQECLKLING"
     gene            complement(3957..>5216)
                     /locus_tag="AC16Y1_03080"
     CDS             complement(3957..>5216)
                     /locus_tag="AC16Y1_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR5831480.1"
                     /translation="RSMVTSMRNIMAQPLPYLEAVRLQFGDVVQFPIPKPATYFVASP
                     HGARDVLVTKHKQVNKKTIQYSSLSLVTGEGLLTADGEVWKQRRRILQPAFHHEMIAL
                     TETEVVRALENLNSRWHDSLKTGEQILDIDQEMMSLALEITGNTLFGTDLSGEALEIT
                     TATISALHGVVARAQNPLALPLKVPTPANQSMNKAVARLDQAIARIIKMRDKDRLPAG
                     SPIRDMLDVLLAAEDVNQINPQQLRDEIATFIVAGHETVASGLTWAIYLLANNQDEQT
                     ALATNPQRSTQVFDETLRLYPPAWLITRRTLDPVVVDETEIPANSLVILSPWVTHRHE
                     SIWEKPNQFISDRMKDGTPQVGYFPFGAGPRLCIGRDMALLEGQKVLADLFGNWKITP
                     LTSGVVPIDASVTLRPVNGLPMRVTSR"
BASE COUNT         1425 a         1124 c         1191 g         1476 t
ORIGIN      
        1 agccaagctt ttaagttagt tccttcttca aaagactgga atgcacgata agccttggcg
       61 aaggtttctt gtactaggtc ttctgcgtct gcttgatttc gggtcaatct gaatgcgcca
      121 gcataaatcg cgtcaagaag cggaagggca tcccgttcaa agcgggccgt taattctgcg
      181 gcttttgctg gcgaaagttt ttttgccgca actgaatcaa tttcgtcagc attcaattgc
      241 gctgatgaga aatcatcatc ggttgctggc gactttgtca tacttaatga ccctactcgc
      301 ttttggttaa ctctgaattt tgcctgctca acaagtagat tttcggcttt ttggaagctg
      361 tcaattacga gcggtggcat taacccggtg gacataagta attcaactga atttttgcta
      421 cagctattcc caatcttcac taaaattcaa atctttatta actactaggc ttaaagggtg
      481 tctgaactgt ggattgcccc taatagggat agcaccattt caaaaactgt ccagattcca
      541 ggctcaaagt cacttacaaa taggtggcta gtactagcgg cgatcgctga cggaccatct
      601 cggattaatt tgccgcttaa ggctcgtgac actgaattga tggctaacgc aatccaatct
      661 cttggaaatt caattgaagt tgatggtcag gcatgggtag ttaccccagg taaattttca
      721 ggctcaactg aaatcgattg cggccttgct ggaaccgtaa tgcgtttcgt tcccccaatt
      781 gcagcattag cttctggagc agttcggttt gatggtgatc ctcgggccag agttcgcccg
      841 atgtccggag tcataaatgc attacgtgca ttaggggctg acatcactga tgacggtcga
      901 ggtactcttc catttgtcat taatgcaaaa ggttttttgc caggtggcca agtcactatt
      961 gatgcttctg cctcttcgca atttgtaagc gcactattac ttgctggcgc tcgattcgat
     1021 gatggcttaa cagttaccca ctcgggtgga aaactacctt ctttaccgca cattgagatg
     1081 acagttgaag ttttggcacg agctggcgta aaagttgcag cggatttaat tgatctacaa
     1141 aatgccacct ggacaatagc gccgcaaatt ccacaaggtt tttcaatcac agtcgagcca
     1201 gatctatcta atgcagcagc atttgttgca gctgcattag ttgctggtgg agaagtcaca
     1261 atcgaaaatt ggccagagca aactacacag gctggaaatg cgctgattga tctaattcca
     1321 atgcttggcg gtcaagtaag ccgcaacggc aacaatttag ttttttcggg aactggcgca
     1381 ataaatggaa tatctgctga cttatcagcc gtaggtgaat tgactccagt tatcgcagca
     1441 atgtgtgcgc ttgcagatac cccgtctgaa ttaacaggca tcggacactt acgtggacat
     1501 gaaacagatc gcctagctgc tttagctacc gaaataaata aaatgggtgg tcaagtcacc
     1561 caaatgcaag acggactgca aattaatcca agttctctgt caggtggggt attcaacacc
     1621 tacgaagatc atcggatggc aatggctggt gcagttttgg gtttgaaagt aaaaaatctg
     1681 caaattgaaa acatatctac aaccagcaaa acactgccag aatttgctca aatgtggttg
     1741 gagttggtta gttagtggtc aattacgacg aggataaatt tgatcggcca agccgattta
     1801 attcacgtcg cagatcaaaa gatcgtccag atttttctac tgctcaagtc ggacaaatta
     1861 tcggtatcga tcgtggccga ataacttgcg tttatccaat tgccaccaat catgaagaac
     1921 aaactcgggt gactgcagtt aaggcacgtg agcttggacg taaaggtgtg acgataggcg
     1981 accaagttcg gctagttggt gacctttcag gtaaagcaga tactttagcc aggctagttg
     2041 aagttttacc gcgcgagact gaactgatga ggaccgcaga tgattctgat ccggtagagc
     2101 gagttatcgt tgcaaatgca gatcagattg caatagtgac ttcaatagcg aatccagaac
     2161 ctcgactggg tttaattgac cgatctttgg tggctgcctt tcattcagga cttaaaccac
     2221 tattggtaat taccaaaaca gacctagcca atccagcgca acttttacaa aattacgagg
     2281 gtatagatct tccttttatt ttggttaata acaaaactgg cgaaggactt aataaattac
     2341 ttgctgaaat aaaaggccac aacactgtat ttatcggtca ctctggagtc ggaaaatcta
     2401 ctttagtaaa tgcattagta ccagatgccc agagaatcac aggagatgta aacgatgtga
     2461 cagggcgcgg taagcacact tcgacatctg caatagccct accgcttcca aatgacactg
     2521 gctgggtaat tgatacacct ggcgttagat cttttggttt agcccacgtt aaaccagagg
     2581 acataattga atcgttccca gaattaatcg atggaactct taaatgtccg cgtgcctgca
     2641 cacacgttaa ttcagttggc cagcggccac cagattgcgc gctagatgac tgggtttcgg
     2701 atgggaatgc cggtgcggct ggactggcta gattaaattc cttacgccga ctcttgttcg
     2761 cccttgagtc gccgtctagc taattcaaaa tgttcctttg taattgagcc acttacccgc
     2821 tttaacacaa gtagtagtac ggcaaaatcg tcagtaaaac caaatcctgc aactaagtca
     2881 ggaattaaat cggcgggcat gatgaaatac gcaagagcgg cagccaaaat tgctttcacc
     2941 ttgaatgggg ttttggaatc ccgaagtgca taataagcag cagtaatttg ctctgcgaat
     3001 gggatcttgc cggctgcagt cagttttttt ggaaaagcat ccagcaccgc ttgtattttg
     3061 tccggattgc ttgaaaagaa atcagtagtt gctttaacag tttctcgaac agctttcttt
     3121 gaatactttt ttcgcccaaa catcagttaa tttctccaaa tttattattc acctaagtct
     3181 attccactag cgttgtagtg tgttcgatct agaatttgca cagaccttgg cagatgttgc
     3241 cgacaaaatc accttgggtc gctatcttgc gcaggattta aagattgaaa caaaaccaga
     3301 tgcaactcct gttaccgaag cagaccatgc taccgaacta aaactacgag aattaattca
     3361 atcaactctg cctgcagatg gaatacttgg tgaagaattt ggccaaacag acggctctaa
     3421 taatcgggtt tggattttag atccaatcga tggaacgaaa aactatttac ggggtgtgcc
     3481 agtttgggcc actttaattg cgctagttga agatgatgag ccaattgttg gtatggtctc
     3541 ctgtcctgca cttggacgca ggtggtgggc cagcaaaaat aacggcgcct ttacaagtga
     3601 ctttgacgga acgattagaa aaatttcagt ttcaggagtg tcaaaaataa acgatgcctt
     3661 tttttcgtac tctgatcgaa tcggttggga aaaattttct ggtgcttttc aaaagttaac
     3721 tgattcagtg tggcgcactc gcgcctatgg tgatttttta tctcacttac tagttgccga
     3781 aggtgcagtg gacattgcgg ctgaacctga attaaagaag tgggacatgg cggcaaacaa
     3841 catcattgtg acggaagctg gtggtttagt aacgggatta aaaggtgggc gcgcctttga
     3901 tgaatttaat gctctttcta ccaatggcaa attacatcaa gaatgcttga aactaattaa
     3961 cggctagtaa cccgcatcgg caaaccattt actggcctaa gggttaccga cgcatcgatt
     4021 ggcacaactc cactggtcag tggagtaatt ttccaattac caaataaatc agcaaggact
     4081 ttttggcctt ccagtagcgc catatctcgg ccgatacata accttggccc agcaccaaat
     4141 gggaaataac ccacctgagg tgttccatct ttcatgcgat ctgaaataaa ttgatttggc
     4201 ttttcccaga ttgattcatg ccgatgagtt acccatggac tcaatatgac tagtgaatta
     4261 gcgggtatct ctgtctcatc aactacaact ggatctaggg tacgtctcgt gatcaaccag
     4321 gcaggtgggt aaagacgtaa cgtttcatca aaaacttgag tactccgctg cggattagtt
     4381 gcaagtgcag tctgttcatc ttggttgtta gcaagtaaat aaattgccca agttaatccg
     4441 ctagcaacag tctcgtgccc agcaacaata aatgtggcta tctcatcgcg cagctgctgt
     4501 ggatttattt gatttacatc ttcagcagct agtaggacat ccaacatgtc tcggattggc
     4561 gaacctgccg gtagtcgatc tttatcgcgc atttttatga ttcgtgcgat tgcttgatct
     4621 aatcgagcaa cagctttatt catactttga tttgctggag ttggaacttt aagcggcaac
     4681 gctagcggat tttgtgcccg agccactacg ccatgcaatg cgctaattgt ggcagtggtt
     4741 atttctagtg cttcgccgct taggtctgtt ccaaataagg tatttcccgt aatctcaagg
     4801 gcaagggaca tcatttcttg atcgatgtcc aaaatctgtt cacccgtttt tagtgaatcg
     4861 tgccatctag aattcaagtt ttctaatgcg cgaactacct cagtctcggt taaggcgatc
     4921 atttcgtgat ggaatgcggg ctgcaaaata cgtcgacgtt gtttccagac ttcgccatct
     4981 gctgtcaaca atccttcacc agtaactagc gatagcgatg aatactggat tgtttttttg
     5041 tttacttgtt tatgttttgt tactaaaaca tcacgagcac cgtgaggcga ggcgacaaaa
     5101 taagttgctg gttttgggat aggaaattga accacgtcgc caaattgcaa tcgaaccgct
     5161 tctaaatatg gcaatggctg cgccatgatg ttacgcatcg aggtaaccat agatcg
//
LOCUS       DBNHVN010000160         1103 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_995614, whole genome shotgun sequence.
ACCESSION   DBNHVN010000160 DBNHVN010000000
VERSION     DBNHVN010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_995614"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="AC16Y1_03085"
     CDS             complement(<1..137)
                     /locus_tag="AC16Y1_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HIR5831481.1"
                     /translation="MLRTHDAGRLRKTDAGLEVQLTGWVSRRRDHGGIAFIDLRDRSG
                     T"
     gene            complement(159..>1103)
                     /gene="hisS"
                     /locus_tag="AC16Y1_03090"
     CDS             complement(159..>1103)
                     /gene="hisS"
                     /locus_tag="AC16Y1_03090"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR5831482.1"
                     /translation="WYTGPNFRYEQPQSGRYRQHTQVGVEAIGSADPALDAEVIWMAS
                     YAQREILGLKKVRLLVNSLGCQNCRPDYRKLLIDFLAKVDLDEATRERAKLNPLRVLD
                     DKREHVQKQLTDAPLMPDNLCIDCSNHHVKVLAYLDQLHVQYELAPRLVRGLDYYVRT
                     TFECLHDGLGAQSAIGGGGRYDGLIETLGGPAIGGVGFGLGVDRTCLAMQAEGLDQST
                     SGETNVFVVPIGSEAFTRCLQLTKELRANGIGTELAFDDRSMKAAMKNADRSGAKFAL
                     IMGEEELVANSAVVKNLFNGAQESVAVDLVAQHLLTNL"
BASE COUNT          289 a          289 c          231 g          294 t
ORIGIN      
        1 atcgttccag accggtcgcg aagatcaata aatgcgatgc ctccgtggtc gcgccgacga
       61 cttacccaac cagtgagttg gacctcgaga ccagcatcgg ttttacggag tctgcccgca
      121 tcatgcgttc gtaacattta ttcctcggta agaagtactt ataaattagt taaaagatgt
      181 tgcgcgacta agtctactgc gacagactct tgtgctccgt tgaacaaatt cttaaccact
      241 gcagaattgg caaccaactc ttcctcaccc attattaggg caaattttgc accacttctg
      301 tcggcatttt tcatcgcagc tttcatgctt cggtcatcaa aagcaagttc ggtgcctatt
      361 ccatttgcac gcagttcttt agttaactgt aggcatcgag tgaaagcttc tgaaccaatc
      421 gggactacaa atacattggt ctcaccactc gtactttgat ctaaaccttc agcctgcatg
      481 gccaagcaag tgcgatcgac acctaagcca aagccaactc caccgatcgc cggaccgcca
      541 agtgtttcga tcaacccgtc gtagcgaccg ccaccaccga ttgctgactg tgcaccgagg
      601 ccatcgtgca aacattcaaa agtagttcgc acatagtaat caagccctct tactaaccgg
      661 ggagctaatt cgtactgaac atgaagttga tctaaatacg caagtacttt cacatgatga
      721 tttgagcaat caatacacag attgtctggc attaaaggtg catctgttag ctgcttttga
      781 acgtgttcgc gtttatcatc gagtacgcgc agcggattta acttggctct ctctcttgtc
      841 gcctcgtcta aatcaacctt ggctagaaaa tcaataagta attttcgata atccggtcga
      901 caattttggc aaccaagtga attaaccaag agtctgactt tcttaagacc tagtatttcg
      961 cgttgtgcgt aactggccat ccaaataacc tcggcatcaa gcgccggatc ggcagaaccg
     1021 atcgcttcaa cgcctacttg ggtgtgttgc cgatagcgtc cagactgcgg ttgttcatac
     1081 cgaaaattag gcccggtgta cca
//
LOCUS       DBNHVN010000161         4924 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_698182, whole genome shotgun sequence.
ACCESSION   DBNHVN010000161 DBNHVN010000000
VERSION     DBNHVN010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4924)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4924)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4924
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_698182"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="AC16Y1_03095"
     CDS             complement(<1..257)
                     /locus_tag="AC16Y1_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR5831483.1"
                     /translation="MPAPICSALENHGIKDAFQIQELTLPLAISGKDLIGQAKTGTGK
                     TFGFGLPLLTRIVSPTSPEWADHKHAGKPQALVIVPTRELAV"
     gene            368..1018
                     /locus_tag="AC16Y1_03100"
     CDS             368..1018
                     /locus_tag="AC16Y1_03100"
                     /inference="COORDINATES: protein motif:HMM:NF025166.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like fold-containing protein"
                     /protein_id="HIR5831484.1"
                     /translation="MTSPAVIDLLGLLAYGELSSFEQMAADAQKAPSLQDKAAMSKFA
                     SGEFEHYTRITSRLKGLGVEPVDAMQPFAAALDGYHQQLVPSNWFEGLVKSYIGEGIA
                     SDFYREASKFLDKETAELVDEVLSDNGFADFAIERIRAACENDAIMAGRLALWGRRMM
                     GEMIAQAAVAASERMTLAQLFVSDQPSNSLADDLNGLVNRLTVGHARRMDVLGFAS"
     gene            complement(1015..1236)
                     /locus_tag="AC16Y1_03105"
     CDS             complement(1015..1236)
                     /locus_tag="AC16Y1_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HIR5831485.1"
                     /translation="MEIRIGIQQALREVALESNESPADVNAKVAEAIKSGSLLHLTDD
                     KGRTITVPGDKIAFVEVGVATTGKVGFAS"
     gene            1469..>4924
                     /locus_tag="AC16Y1_03110"
     CDS             1469..>4924
                     /locus_tag="AC16Y1_03110"
                     /inference="COORDINATES: protein motif:HMM:NF037833.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin-like domain-containing protein"
                     /protein_id="HIR5831486.1"
                     /translation="MAFALTSLFLVAASENSQATSSPTVSAAVTTVRVGSTTNLNLSI
                     SNFDSTKSFQVTVKLVDANNEDVTNGTIEATKFGSSTNISGYSSVNGSGKFSGTKIGF
                     KGTYSEVIQSLATVTWNANSAANVKMRVAIATAPDANEFFDGNSGHYYRYVSGSNITW
                     GAAAQNAKTLSKLGLQGYLATITTKAENEFIGQETSASNIWFGLTDGMKAETNGTFTT
                     DTSIEGKWQYIDGPANEKNFVYHTYSGTSSIPTSSATGYTSGTWYTATSNSPSASGYL
                     AWASGEPNGGTSESCAVTNWNSVKGRWNDLTCNYSATGYLVEFGGLASDAFTGQNATV
                     DLNLVASNSALAPSNSVIPTIDSVTSIGSTVTASDGTWSDNGATITSTTYKWQYGDGL
                     GAWTDVPTLDGTSITNQSLVIPRSTAGYKIRVLVSKTNSVGTSDPTASQASASIVTGV
                     SLAPTLNSLTASNGALSVVLTHGDNGGSSISAVKYQLSTNGASWGSAVTVNSASSPFS
                     ISGLTNGTLYYVKVLSVNGVGDSPYSNVLSATVGQVPDPPTNVSVVADNDRFTLRFTG
                     STTYGSAVTSYQYSTDGGSTWKALSGTASPQYITTASDDSALTSGTSYDVRMRAVSAI
                     GNSSSTSSLSSTFRSESAFSTSNFVLGGTSGNPASYDSLTGAYVLTQAANNRYGTAWS
                     TSRLDLRQDFVINASVNLGSSEGGADGMAFVIQPASTNSGSSGGGLGYAGINNSFAVE
                     FDTYFNNPGDSTSSDHITYIANGAAGTSSAHTAYVAPYTSELEDGTWRDVRFTWIASS
                     KTLITEFETVSDGPLVQILSTSNLNLTSSGLINSDFAYWGFTAATGGAVNKQQVKINS
                     YTVSNRPNNSPTLDAISDLTISSALNSNSVVLTFADDSSTQAQFTILGTSSNQSIVAN
                     ADIIETVSSTTAGDLAITPKGVSGSTTITVKITDADGANATKTFVLTVFGPPSVPRNV
                     STSLSGTTATISWLAPESNGGSPISRYVAYATPQTGNALSCNTTSSGSNLPARSCEIS
                     GLIEGTRYTVTVVAENTELSNVLPAQTSAASAGIGLATIKSSIPGNIPSRITPVAPPK
                     FVGDLTEATKQTLVNAGVVVPPSGAPVVNVSSDLSTTSINTQTNVTLTETVTSGSEIV
                     LEVVPP"
BASE COUNT         1385 a         1058 c         1098 g         1383 t
ORIGIN      
        1 accgctaatt cgcgggtagg aacgataact aaagcctgtg gtttacctgc atgtttatgg
       61 tcagcccatt caggtgaagt tggcgaaaca attcgagtta gtaaaggcag gccaaagcca
      121 aaagtctttc cagtgcctgt ttttgcttga ccaattaagt ctttgccaga aatagcaagt
      181 ggcaatgtaa gttcttggat ttgaaatgcg tctttaatgc catgattttc caacgcacta
      241 caaattggtg ctggcacgcc gagttcggca aaagtaggga taagtgctcc tgtgtatttc
      301 tggataaaat caacgttttt cgcaggtaat tacatcgtac ctgtgatgtg cggataacct
      361 aaacgttgtg accagtccag cagttattga tttactcggc ttacttgctt acggcgaact
      421 ttcatcgttc gagcaaatgg ctgctgatgc acaaaaagcg ccctcgcttc aagacaaagc
      481 tgcgatgtca aagtttgcct cgggggagtt cgaacattac accagaatca cttcgcgcct
      541 taaaggttta ggtgttgaac ccgtagatgc gatgcaacct tttgccgctg cacttgatgg
      601 ttaccaccaa cagttagtgc caagtaattg gtttgaagga ttagttaagt cttacatcgg
      661 tgaaggcatt gcaagtgatt tctatcgaga ggcttcgaag tttcttgata aagaaaccgc
      721 tgaattagtt gacgaagttt taagcgacaa tggttttgca gattttgcaa ttgagcgcat
      781 tagagctgcg tgtgaaaatg atgcgatcat ggccggccgt ttggctttgt ggggaagacg
      841 catgatgggt gagatgattg ctcaagcagc tgttgcagca agtgaacgca tgacacttgc
      901 tcaattattt gttagcgatc aaccaagtaa ttcacttgct gatgatctga atggtttagt
      961 taatcgatta actgttggac atgcgcgcag gatggatgtt ttaggttttg ctagttaact
     1021 tgcaaagcca actttgccag tagttgctac gccaacttca acgaaagcaa ttttgtcgcc
     1081 cggcacagta atagtgcgac ccttgtcgtc agttaagtga agtagagatc cagatttaat
     1141 tgcttccgca actttggcat ttacgtcagc aggagattcg ttactttcca acgcaacttc
     1201 acgtagagct tgttgaatac cgatccgaat ttccatggtt ctccttttag ggggtcttac
     1261 ggtttccacc ttaaatgacc tctatatagg gtgttcaccg acttcactaa gggcaaaatg
     1321 ttcggctagc gcatcacaaa ctcgggaaac ccgactgtgg gaactaaatc ctttatttat
     1381 cagggtaaat ttgagtttgg cttactgatt tagccaattt ctagttcggg ggaagttaga
     1441 gatgcttaga aaggccacta atttttttgt ggcttttgca ctaacctctc tttttcttgt
     1501 tgcggcttct gaaaacagtc aagcaacctc ttcacctact gtttcggctg cggttacgac
     1561 agttcgagtc ggctccacaa caaatttgaa tttgagtatt tcaaattttg attcaactaa
     1621 aagctttcaa gtaaccgtca aattggttga tgcaaacaac gaagatgtca caaatggaac
     1681 tattgaggca acgaaatttg gctcttcaac caacatttcg ggttacagct cagtaaatgg
     1741 ctcagggaaa ttttctggaa caaaaatagg attcaagggt acttattcag aagttattca
     1801 atctcttgcc actgttacct ggaatgcaaa ttccgcggct aacgtaaaga tgagagttgc
     1861 aattgcgact gcaccagatg cgaatgaatt ttttgatggt aactcagggc attactatcg
     1921 atatgtttca ggcagcaata taacttgggg tgctgctgcg caaaatgcaa aaacactctc
     1981 aaaacttggg ctgcaaggct atcttgcgac gataactaca aaagctgaaa acgaatttat
     2041 tggccaagag actagtgcaa gcaacatctg gtttggattg accgatggaa tgaaagctga
     2101 aactaatgga acctttacga ccgatacttc aatagaagga aaatggcagt atatagatgg
     2161 accagctaat gaaaaaaact tcgtttatca cacttattca ggaacgagtt caattccaac
     2221 ctcgtctgca actggctata cctcgggcac ctggtacaca gcgacaagta attctcctag
     2281 cgcatctggt tatttggctt gggcgtcagg ggaaccaaac ggcgggacat cagaaagttg
     2341 cgctgtaaca aactggaata gcgtaaaagg aagatggaac gatttaacct gcaactattc
     2401 agcaactgga tatttggttg agttcggcgg tttggcttcc gacgctttta ctggacaaaa
     2461 tgccacggtg gatttgaatt tggtagcttc aaattcggca cttgcacctt caaactccgt
     2521 catcccaacg atagattccg taacttctat tggctcaact gttacagcga gtgatggtac
     2581 ttggtcagac aatggtgcaa cgatcacttc gactacgtac aaatggcaat acggtgatgg
     2641 tctcggtgca tggactgatg taccaacttt agatgggaca agtatcacta atcagtctct
     2701 tgtgattccg agatcaaccg ctggttacaa aataagagtt ttggtctcta aaacaaactc
     2761 agtgggcaca agtgatccaa ccgcctctca agcatcagca agtattgtca ctggagtttc
     2821 ccttgcgcca acattaaatt ctttaacagc aagcaatgga gctctaagcg ttgtgctaac
     2881 tcatggtgat aacggcggta gttcgataag cgccgttaag taccaactct cgactaatgg
     2941 agcctcttgg ggcagtgccg taacggtaaa ttcagcgtct tcgccttttt cgatttctgg
     3001 attaaccaat ggaactctct attacgtaaa agtgttgagt gtaaatggag ttggagatag
     3061 cccatattcg aatgtgctta gtgccacggt tggacaggtt ccagatccac cgacaaatgt
     3121 ctcagtagta gcagataacg acagatttac tttaaggttc acaggatcaa ccacatacgg
     3181 ttctgcggta acgagctatc aatattcaac tgatggtggc tcgacttgga aagcgctaag
     3241 tggcaccgct tcgcctcaat acataacaac cgcaagcgat gattcagctc tcacatccgg
     3301 cacaagctac gacgtaagga tgcgcgcggt tagtgcaatt gggaatagtt caagtacttc
     3361 aagtttgtca tcaaccttta gatccgaatc tgcattttca acttctaatt ttgtattggg
     3421 aggcacgtct ggaaatccag ctagttacga ctccttaact ggtgcatatg tgttaactca
     3481 agcagccaat aatcgttatg gcactgcttg gagtacctca cgcttagatt tgcgtcaaga
     3541 ttttgtcatc aatgcatctg tcaacttggg tagtagtgag ggaggtgccg atggtatggc
     3601 atttgtcatc cagcccgctt ctacaaatag cggatcaagc ggtggtggac ttggttacgc
     3661 gggaataaat aactcattcg cggttgaatt tgatacgtat tttaacaatc caggtgactc
     3721 aacttcctca gatcacatca cttacattgc taatggcgct gctggtacat cctcagctca
     3781 cactgcctat gtagctccgt acacatctga actcgaagat ggaacttggc gtgatgttag
     3841 attcacttgg attgcaagct ctaaaacttt gattacagaa tttgaaacag tttcggatgg
     3901 accattggtt caaattctta gtacgagtaa tttaaatctg acatcttctg gattaataaa
     3961 ttcagatttt gcatattggg gatttaccgc tgcaactggt ggagcagtta ataagcaaca
     4021 agtcaaaatt aactcgtaca ctgtcagcaa tcgacccaat aactcaccga cgcttgacgc
     4081 gataagtgac ttaactatta gttctgcact caattcgaat tctgttgtgc tgacttttgc
     4141 agatgattct tcaacacagg ctcaattcac aattcttggc acgtcttcaa atcaatccat
     4201 tgttgcaaat gccgacatca ttgaaactgt tagttctaca acagccggag atttggccat
     4261 tactccaaaa ggcgtatcag gttcgactac gatcaccgta aaaattacag atgctgacgg
     4321 tgctaatgcg actaaaactt ttgtcctgac agttttcggt ccaccatctg tgccaagaaa
     4381 tgtttcgacg tctttatcag gaacaacagc aaccatttcc tggttagcac cagagtcaaa
     4441 cggtggttca ccaattagta ggtacgtggc ctatgcaaca cctcagactg gaaatgctct
     4501 tagctgcaat acgacaagct caggttcaaa tctgccagcg cggtcatgtg aaatttctgg
     4561 attaatcgaa ggaactcgtt acacggtcac agttgttgca gaaaatactg agttatcgaa
     4621 tgttttgcca gcacaaacct ccgcggctag tgctggaatc ggtttagcaa ctataaaatc
     4681 atctattcca ggaaacattc catcgcgcat cactccagta gcaccgccta aatttgttgg
     4741 cgatcttacg gaagcaacaa agcaaacatt agtaaatgca ggcgtagttg ttcccccaag
     4801 cggagctcca gtagtaaatg tatccagtga cctaagtacc acttcaatca atactcaaac
     4861 aaacgtcacc ctgacagaaa cagtcacttc cggttctgaa atcgttttgg aggtagtacc
     4921 gcca
//
LOCUS       DBNHVN010000162         3331 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1368356, whole genome shotgun sequence.
ACCESSION   DBNHVN010000162 DBNHVN010000000
VERSION     DBNHVN010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1368356"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1637)
                     /gene="acnA"
                     /locus_tag="AC16Y1_03115"
     CDS             complement(<1..1637)
                     /gene="acnA"
                     /locus_tag="AC16Y1_03115"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015036993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIR5831487.1"
                     /translation="MSSLNSFGAKTNFKSGATEIEIFSLSALSGVERLPYTHKVLLEN
                     LLRTEDGANVTAKQIESLANWDASSQPTDEIQFTPARVIMQDFTGVPCVVDLATMREA
                     FTKLGGDPKRINPLAPAELVIDHSVIADFFGREDAAQANVELEYQRNGERYQFLRWGQ
                     DAFTDFKVVPPGTGIVHQVNIEALARVIMVRDGLAYPDTCVGTDSHTTMVNGLGVLGW
                     GVGGIEAEAAMLGQPVSMLIPRVVGFKLFGSLPAGTTATDLVLTITAMLREHGVVGKF
                     VEFYGAGVSAVSLANRATIGNMSPEYGSTAAVFPIDDETLNYLRLTGRSEEHIALVKD
                     YAQVQGLWHNSAVEPTYSEYLELDLATVVPSIAGPKRPQDRIELAKAASAFTQALSTY
                     TDKPDKRATVAQSNVEYEIGHGSVVIAAITSCTNTSNPSVMLAAGLLAKKAVEKGLTR
                     KPWVKTTLAPGSQVVTDYYNKAGLTPYLDKLGFNLVGYGCTTCIGNSGPLDPEISTAI
                     QESDLAAAAVLSGNRNFEGRINPDVKMNYLASPPLVVA"
     gene            complement(1700..2917)
                     /locus_tag="AC16Y1_03120"
     CDS             complement(1700..2917)
                     /locus_tag="AC16Y1_03120"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HIR5831488.1"
                     /translation="MNESTEQARVILGDEITLRIDKVANGGFCIGRHNGQAVFVRHSL
                     PGELVKAEITEVNKRYLRADVTEVIEPSSHRASPPCQYAGVCGGCDWQHSEYDFQLEL
                     KSSVVIEQLAHLGQVEFVNEKPLSEFKVRSLLPNETGLHWRTRNRYFNIAGYSLGMRM
                     SRSHSAVEIVDCLIAVPGSVELAKSALHLGTKEIATAQSSTDQLVLVDQNGGPWLDEI
                     VEDRSWRIHAGSFWQVHKDAPTVFVNQVRKFAQPKPADRLLDLYAGAGLFAATLAKDV
                     GAKGEVVAVESSINAVRNARRSCSDLGNLTLITSDVGQWLLQNDQRKAHKEFDVVVLD
                     PPRTGAGQEVISKIAQLAKRSIVYVACDPASLGRDTGYLKESGWEIAELVGFDAFPNT
                     AHVECIAKFIPAK"
     gene            complement(2917..>3331)
                     /locus_tag="AC16Y1_03125"
     CDS             complement(2917..>3331)
                     /locus_tag="AC16Y1_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HIR5831489.1"
                     /translation="AYARATRPTTLEAVTVAIDPKETERFIEEWERRKIPVTLTVLHS
                     PYREISRPILDYVRSIARQSSRDLITIYIPEYVVGKWWEQLLHNQNALRLKGRLLFTP
                     GVMVVSVPWQLASSELVDLDQRMSDRDEYYLPPTS"
BASE COUNT          888 a          788 c          703 g          952 t
ORIGIN      
        1 gccacaacta aaggtggtga agcaaggtaa ttcattttga cgtcaggatt tattcgacct
       61 tcgaaattac ggtttccaga caacactgcg gcagcagcca gatcactttc ttgtattgct
      121 gttgaaattt caggatcgag tggtccagag tttccgatac aagttgtgca accgtagcca
      181 actaaattga aaccaagttt gtctagataa ggcgttagcc cagctttgtt gtagtaatca
      241 gttaccactt gtgagccagg tgcaagtgtt gtcttaaccc acggtttgcg ggtcaacccc
      301 ttctctacag cctttttagc aagcaagcct gcagccaaca tgacagatgg attcgaagta
      361 tttgtgcaag aagttattgc ggcaataacg actgaaccat gtccaatttc atattccaca
      421 ttgctttgtg ccaccgtcgc gcgcttatcg ggcttgtctg tatatgttga aagtgcttga
      481 gtgaaggcgc ttgcagcctt ggctaattca attcgatcct gtgggcgctt tgggcctgcg
      541 attgacggaa ctactgttgc taaatcaagt tcgaggtatt cactgtaagt cggttctact
      601 gctgagttat gccaaagacc ttgaacttgt gcgtagtctt tcactaaagc aatgtgttct
      661 tcacttcgtc cagtaagtct taagtaattt aaagtttcat catcaatagg aaatactgcg
      721 gcggttgatc catactctgg actcatgttt ccaatagttg cccgattagc taatgaaact
      781 gcgcttacac cagcaccata aaactcgaca aactttccaa ctactccatg ctcacgaagc
      841 attgccgtaa ttgtcagaac taaatcagtt gcggtagtac ctgcaggtag tgagccgaac
      901 aatttaaatc cgacaactct tgggataagc atcgaaactg gctggccaag cattgctgct
      961 tcagcttcga tcccacctac gccccaaccc aatacgccca agccattaac catggttgtg
     1021 tgtgagtcag ttccaacaca ggtgtctgga tacgctaatc catctcgaac cataatgact
     1081 cgagctagtg cctcgatgtt aacttggtgc acaattccag tgccaggcgg aactactttg
     1141 aaatcggtaa aagcatcttg gccccaacgc aaaaattgat accgttcccc atttcgctga
     1201 tactcgagtt caacatttgc ttgggcagca tcttcccgac caaagaaatc agcaattacg
     1261 gaatggtcaa tcacaagttc ggccggtgca agaggattaa ttcgttttgg atctccacca
     1321 agttttgtga atgcttcacg cattgttgct aaatcaacaa cacatggaac tccagtgaaa
     1381 tcttgcataa ttacccgagc gggagtgaat tgaatctcat cggttggctg cgaactggca
     1441 tcccagttgg caagtgattc gatttgcttt gcagttacat ttgcgccgtc ttctgtacgc
     1501 agtaaattct cgagtaaaac tttgtgagta tatggaagtc gctcaacacc actcaatgcc
     1561 gacagcgaaa aaatctctat ctcagttgca ccggatttga aatttgtttt tgcgccgaat
     1621 gagttaaggc tagacatact gctcctagaa gtatcttgac gtcaagatac ttcaattcta
     1681 gtgcattact tgttttaagt tacttggctg gaatgaactt ggcgatacat tccacatgag
     1741 cagtgtttgg gaatgcgtcg aatccaacaa gttccgctat ttcccagcca ctctctttta
     1801 ggtagccagt gtctcgtcca agagatgcag ggtcacatgc gacataaact attgaacgct
     1861 tggctagttg agcgatcttg gaaataactt cttgaccggc tccagttctt ggtgggtcta
     1921 agacaacgac atcaaattct ttgtgtgctt tcctttggtc attttgaagt aaccactgtc
     1981 ctacatcgga tgtgatcaaa gttagatttc ccaagtcact gcaagatcta cgggcgttgc
     2041 gcactgcgtt tattgagctt tcaactgcca caacttcacc tttagcacca acatcttttg
     2101 ccaaggtggc tgcaaataat cctgcaccag cgtacaaatc aagcaagcga tcggctggtt
     2161 ttggttgcgc gaattttcga acttgattca cgaaaacggt tggtgcatct ttgtgtacct
     2221 gccaaaaaga cccagcatgt attcgccaag atcggtcttc aactatttca tcaagccaag
     2281 gaccaccatt ttgatcaact aaaacaagtt gatcagttga actttgcgca gtggctatct
     2341 ctttcgtacc caagtgcaat gcagattttg ctagttctac tgagccgggt accgcgatta
     2401 aacaatctac aatctcaact gcagagtgag acctagacat tcgcatgcca agcgaataac
     2461 cggctatgtt gaaatatcta ttgcgagtac gccaatgaag tccagtttcg ttaggtaata
     2521 aacttcttac tttaaattcg cttaatggtt tttcattaac aaattcaacc tgtcccaggt
     2581 gggccaactg ctcaatcacg actgaactct ttaattcgag ctggaaatcg tattcactat
     2641 gctgccaatc acagccacca cagactcctg cgtactgaca aggaggactt gctcggtgtg
     2701 agcttggctc aattacctcg gtgacatctg ctcgcaagta ccgtttatta acttcagtta
     2761 tctctgcttt tactaattca ccgggcaaac tatgtctaac gaacacagcc tgaccattgt
     2821 ggcggcctat gcagaagcct ccattagcga ccttatcgat tcgaagcgtt atctcgtcgc
     2881 caagtatcac tcttgcttgt tcagttgatt cgttcattaa cttgtgggcg gaagatagta
     2941 ctcatcgcga tctgacatgc gttgatctaa gtccactaat tcacttgatg caagctgcca
     3001 agggaccgaa acaaccatta caccaggagt aaacagcaaa cgtcctttca gtcgaagggc
     3061 attttgatta tgtaaaagtt gttcccacca cttacctaca acgtactcag gaatgtagat
     3121 ggtaattaaa tctctagaac tttgtctagc gattgaacga acgtaatcca atatcggtct
     3181 actaatctca cgataaggag aatgtaatac ggtcaatgtg acagggattt tgcggcgttc
     3241 ccattcttcg ataaatcgct cagtttcttt agggtcaatg gcaacggtta ccgcttctaa
     3301 agtagtcggc cttgttgccc tggcataagc c
//
LOCUS       DBNHVN010000163         2061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_476688, whole genome shotgun sequence.
ACCESSION   DBNHVN010000163 DBNHVN010000000
VERSION     DBNHVN010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_476688"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /gene="secF"
                     /locus_tag="AC16Y1_03130"
     CDS             <1..772
                     /gene="secF"
                     /locus_tag="AC16Y1_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR5831490.1"
                     /translation="DQSVEVSKALASALGVNEQDVKIQLVGPTWGSEVSQNAARALVV
                     FLVLVAIFLAIYFEWPMAIAALIALAHDVIITVGIYALSGFEVSPATVIGFLTILGYS
                     LYDTVVVFDKVKENTKGITAGSRMTYSDAANFAVNQTLVRSINTTIVAILPVAAILVV
                     GIIQLGSGTLNDLSLALFVGMIVGTFSSIFIATPFLAQLKERQPEMKALRKRVVSKRG
                     KESDITVTGDALQSSTIVKSVGPRQQRVRRTRSSRKGK"
     gene            798..1331
                     /locus_tag="AC16Y1_03135"
     CDS             798..1331
                     /locus_tag="AC16Y1_03135"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HIR5831491.1"
                     /translation="MNKHEVVALIAQIQDYPKPGVLFKDVTPISANPKAVDFINNQIT
                     SHFGQAKITKIVGVEARGFILGASLATHMNVGFVPIRKQGKLPRKTYRQEYELEYGVD
                     AVEIHTDALDQNDRVLIVDDVLATGGTATAAARLIEQCGAIPVGIAVLLNLDFLGGDV
                     KLKESYPEIDILAVLND"
     gene            1357..>2061
                     /locus_tag="AC16Y1_03140"
     CDS             1357..>2061
                     /locus_tag="AC16Y1_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR5831492.1"
                     /translation="MSAIEGLKSSVALPVKERFPGGVTDQVPDQLQQVIDTLKSFHPN
                     ADVAKVIQAYQVADFLHRNQMRKSGEHYITHPIAVSGMLAELGMTEPTIIAALLHDTV
                     EDTEYDLSQLESDFGSEVAGLVDGVTKLDKVKYGQTSAAETVRKMVVAMARDIRVLVI
                     KLVDRQHNMRTLSYLPIEKQEQKARETLEIYAPLAHRLGMNTIKWELEDLSFATLYPK
                     MYDEITRLVAQRAPARD"
BASE COUNT          595 a          423 c          501 g          542 t
ORIGIN      
        1 ggaccaatcc gttgaggtca gcaaagcatt ggcatctgca ctaggcgtaa atgaacaaga
       61 cgtaaagatt caattagttg gaccaacctg gggtagtgaa gtatcgcaaa atgctgctcg
      121 agcactcgtt gttttcttgg tattagttgc aatattcctt gcgatatatt tcgaatggcc
      181 gatggcgata gctgctctta ttgctctggc acatgacgta atcatcaccg tgggtatcta
      241 tgcgttgtct ggatttgaag tttcgccagc tacggtgatt ggatttttga ctattctggg
      301 ttactcgctt tatgacacag tggttgtttt tgacaaggta aaagaaaata ccaaggggat
      361 taccgccgga agcagaatga cctacagcga cgcggctaac ttcgctgtta accaaacttt
      421 ggttagaagt atcaacacca caattgtggc aattttgccg gtagctgcga ttcttgttgt
      481 cggaatcatt cagttaggca gtggaacgtt aaatgattta tcgctcgcac tatttgttgg
      541 catgattgtc ggaactttct catcaatctt tattgctact ccttttcttg ctcaactaaa
      601 ggagcgccaa cctgaaatga aggcgttgcg taaacgagtg gtcagtaagc gcggaaaaga
      661 aagcgatatc acggtgactg gtgatgcact gcaaagttcg acaattgtta aatcagtagg
      721 gccaagacag caaagggtga gacgaactcg ctcttctcgc aaaggcaagt aatgaaatta
      781 ggaaatgaaa ataagtaatg aataagcatg aggttgtcgc attaattgcg cagattcagg
      841 actatccgaa acctggtgtg ctttttaaag atgtgactcc catctctgct aatccaaaag
      901 ctgtagattt cataaacaac caaataactt cacatttcgg gcaggccaaa ataaccaaga
      961 tagttggggt tgaagctcgc ggctttattt tgggtgcgtc gctagcaacg cacatgaatg
     1021 tcggtttcgt tccgatccgc aagcaaggaa aacttcctcg gaagacttac cgccaagaat
     1081 atgaacttga atatggggta gatgcagtcg aaattcatac tgacgcacta gatcagaatg
     1141 atcgggtact aatcgttgat gatgtcttag caaccggcgg aactgcaacg gcagctgcca
     1201 gattgatcga acagtgtggc gctatcccag ttggtatcgc agttttgctt aacttagatt
     1261 ttcttggcgg cgacgtaaaa ttaaaggaat catatccaga gatagatatt ctggctgtac
     1321 ttaacgacta gttaccgatg aggaaaagag aagcggatga gcgctatcga aggactgaag
     1381 tcttcggtcg cgctgccggt taaagagcgt tttccaggtg gggtaactga tcaggtccct
     1441 gatcagctcc agcaagtaat tgatacgtta aaaagcttcc accccaacgc cgatgtggcc
     1501 aaggttattc aggcgtatca agttgctgat ttcctacatc gaaatcaaat gagaaagtct
     1561 ggtgaacatt acatcaccca tccaattgcc gtatctggca tgttggctga attaggtatg
     1621 accgaaccca caatcattgc tgcgttactc catgacacgg tagaagacac cgagtacgac
     1681 ctgtcgcaac tcgagtcaga ctttggaagt gaagttgctg gattagttga tggggtaact
     1741 aaattagaca aagttaagta tggtcaaacc tcagcagccg aaacggtgcg caaaatggtg
     1801 gttgcgatgg ctcgtgacat tcgagtactc gtgatcaaat tagtagatcg tcaacacaac
     1861 atgcgaacgc tgtcttattt accgattgag aagcaggaac aaaaagcgcg agagacatta
     1921 gaaatatatg cgccacttgc tcatcgactt ggaatgaaca cgattaaatg ggaactagag
     1981 gatctttctt ttgccacttt atatcccaaa atgtacgacg aaatcactcg gttggtggct
     2041 caacgcgcac cagccagaga t
//
LOCUS       DBNHVN010000164         2906 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1590493, whole genome shotgun sequence.
ACCESSION   DBNHVN010000164 DBNHVN010000000
VERSION     DBNHVN010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1590493"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..985
                     /locus_tag="AC16Y1_03145"
     CDS             350..985
                     /locus_tag="AC16Y1_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HIR5831493.1"
                     /translation="MSGIQVPGLSLPESRTPAPNGQGFGDSVFQRLLQDRIIFLGSQV
                     EDQMANTICAQILLLAAEDPEKDIFLYINSPGGSVSAGMAIYDTMQFVKNDVATVAMG
                     LAASMGQFLLCAGAPGKRYSLPHARIMMHQPSGGIGGTASDIKIQAEQMMYTKTKMAE
                     LIAGHTGQSVATITEDSDRDRWFTADQAKEYGFVDHVVKSARDVAGEGGTA"
     gene            985..1611
                     /locus_tag="AC16Y1_03150"
     CDS             985..1611
                     /locus_tag="AC16Y1_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HIR5831494.1"
                     /translation="MTNFDYSPQARYILPSYIERTPQGIREYNPYAKLFEERIIFLGV
                     QIDDASADDVMAQLLVLESLDPERDIQIYINSPGGSYTAMTAIYDTMQFVKCNIQTVC
                     LGQAASAASVLLAAGTPGKRYALPHARVLIHQPYTEGGGQGSDIEIQANEILRMRDEM
                     ESIFSKHTGKDVAEIRRDIERDKILTADQAKEYGIIDQVLTSRVPLTQ"
     gene            1733..2906
                     /gene="clpX"
                     /locus_tag="AC16Y1_03155"
                     /pseudo
     CDS             1733..2906
                     /gene="clpX"
                     /locus_tag="AC16Y1_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020136161.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
BASE COUNT          818 a          614 c          696 g          778 t
ORIGIN      
        1 tgaaagcttt gtcactagtc cttgaaggcg taaaagttac tgataaatct ggaaatgctg
       61 ttgatttaga gtcagtactt aagccaaatc agtcagttga agaattcatc tctaattttg
      121 ctaccgaatc agatgaagag gattctgacg aggccaaata gtctttgtcg atcagaaacc
      181 aatttcacaa agtagcaaag ttcgttaact gcgaaattgc aaatttcaca cccttactgg
      241 aacctttttt gcgattccca aacgctcata gcgaaaaacc ttgagtctgc catgcattag
      301 tcagttgcct gatttagggt tgagacaaca cgtaactaga ggagtcgtct tgagcggaat
      361 ccaagtaccg gggttatcac ttccggaatc gcggacccct gcaccgaatg ggcaaggctt
      421 tggagattcg gtgtttcagc gcctactcca agatcgaatt attttcttgg gttcacaagt
      481 agaagatcaa atggcaaaca ctatttgtgc acagatctta ttacttgcag ctgaagatcc
      541 agaaaaagat attttccttt acatcaactc tccaggcgga tccgtaagtg ccggtatggc
      601 aatttatgac accatgcagt ttgttaaaaa tgatgttgca acagttgcaa tgggcttggc
      661 cgcttcaatg ggacagttct tattgtgtgc cggagcacca ggtaagcgtt actcgctgcc
      721 acatgctcga attatgatgc accaaccttc tggtggtatt ggtggaactg catcagacat
      781 caagattcag gctgaacaaa tgatgtacac caaaactaag atggctgaat taattgctgg
      841 tcatactggc caatcagtag ccacaattac cgaagattca gatcgagatc gttggtttac
      901 cgccgatcaa gctaaagaat atggatttgt tgaccacgtg gtaaaaagtg cccgtgacgt
      961 tgctggcgaa ggtggaactg cgtaatgacg aattttgatt actctccgca ggcacgttac
     1021 atcctgccta gttacatcga acgtacgccg cagggtatcc gtgagtacaa cccttatgcc
     1081 aagttatttg aagagcgcat tattttccta ggcgttcaga ttgatgatgc ttcggcagac
     1141 gatgtaatgg ctcaactttt agtacttgag tcactagatc cagagcgtga tattcagatt
     1201 tatatcaatt caccgggtgg ttcgtacact gcaatgacgg caatttacga cacgatgcag
     1261 tttgtaaagt gcaacattca aacagtgtgc ttaggccaag cggcatcagc agcctcggtg
     1321 ctattagcag caggtacacc aggtaagcgc tatgcattgc cacatgctcg cgtcctaatt
     1381 caccagccgt acaccgaagg tggcggtcaa ggttctgata tcgagattca ggcaaatgaa
     1441 attcttcgga tgcgcgatga gatggaaagt attttctcaa agcacaccgg aaaagatgtt
     1501 gccgaaattc gccgtgacat tgaacgcgac aagattctga ctgctgacca ggccaaggaa
     1561 tacggcatta tcgaccaggt tttaacctct cgggtgccgt taactcagta actaaaccta
     1621 gttatccatg taagtctggg cgtgtgcgct gcgcgaatgt cactaagtcg agatattgtt
     1681 gaaaaacgcc cgagcgtcgg gcaacactta tttcccccag gaggtgcgca ccatggctcg
     1741 aattggcgag ggtggcgacc tattgaagtg ttcgttctgc tgaaaaagtc agaagcaagt
     1801 taagaaatta attgctggac ctggcgttta catctgtgac gagtgcatcg acttatgtaa
     1861 tgaaattatc gaagaagaac tcggcgagag cgtaaatctt ggttgggatg aattacctaa
     1921 gccgcgcgag atctattcat tcttggatca gtatgtaatt ggtcaagagg ttgcaaagcg
     1981 ggcgctttct gttgccgttt ataaccacta caagcgggtt caatctggtg ccaatgaatc
     2041 atcacgcgat gaagcagttg aattagctaa atcaaatatt cttttacttg gcccaactgg
     2101 ctcaggtaaa actttgttag cgcaaacttt ggctcggatg ctgaatgtgc cgtttgcaat
     2161 cgctgacgcc actgcactta cagaagctgg ctacgttggc gaagatgtgg aaaacatcct
     2221 gcttaagtta attcaggctg ctgattacga tgttaagaaa gctgaaacag gcattatcta
     2281 tatcgacgaa atcgataagg ttgcccgaaa gagcgaaaat ccgtcaatca ctcgtgacgt
     2341 ttcaggtgaa ggcgtgcagc aagcactgct aaaaattctt gaaggcacaa ctgcatcagt
     2401 accgccacaa ggtggtcgta agcacccaca tcaggaattt attcaaatcg ataccagcaa
     2461 tgttttattt attgttggtg gagcttttgc tggacttgat gaaattatca agcaacgtgt
     2521 tggcaagaag gctttaggat tcaccgctgc agtggaaact gaaaaaatga gcgaaaccga
     2581 tatttttgct caagttatgc cagaagatat gttgaagttt ggcatgattc cagaattcat
     2641 tggccgtctt ccggtgttta cttcagtatc gcaacttgat caaaaagcac tagttgccgt
     2701 attgactgaa ccaaagaacg cacttgttcg tcagtatcaa aaactgtttg atcttgatgg
     2761 cgttgaattg gaattctcag aagatgcact tgaggcggta gcagatcaag caatccttcg
     2821 cggcactggt gcccgtggtt tgcgcgcaat catggaagaa gtacttcaac ctgtgatgta
     2881 tgacattcca agtcgcgaag atgtcg
//
LOCUS       DBNHVN010000165         2595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_550903, whole genome shotgun sequence.
ACCESSION   DBNHVN010000165 DBNHVN010000000
VERSION     DBNHVN010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_550903"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..562
                     /locus_tag="AC16Y1_03160"
     CDS             29..562
                     /locus_tag="AC16Y1_03160"
                     /inference="COORDINATES: protein motif:HMM:NF025396.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4245 family protein"
                     /protein_id="HIR5831495.1"
                     /translation="MAKRRGRETAYDMVLSMGAVFVAVAVILVIAWRPAKDFIAPVDY
                     NGAIDSAVVNSNWPIYIPKVIPENFEVTSARFERESYGDSRDTRWYLGFASADEFISL
                     WQSDGDFAQIKSATIPNAQCTESKVIAEQSWQQCTSLASATNGYVRTDGELIYLVSGT
                     VDWAELETFIASLEIAK"
     gene            593..802
                     /gene="xseB"
                     /locus_tag="AC16Y1_03165"
     CDS             593..802
                     /gene="xseB"
                     /locus_tag="AC16Y1_03165"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020525704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR5831496.1"
                     /translation="MAANKSDLSYEQAKAALDEVVAKLEDKDLPLDDMVTLWEQGEKF
                     AAICEAKLSAAKAKLDALRPEVDDD"
     gene            complement(799..2019)
                     /gene="xseA"
                     /locus_tag="AC16Y1_03170"
     CDS             complement(799..2019)
                     /gene="xseA"
                     /locus_tag="AC16Y1_03170"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR5831497.1"
                     /translation="MSASDEFKLGISAESPFAVHQVSALVSDWIGRLGSVWVEGQLIE
                     VKTGRGLTYLTLRDVQSEEVLKMYMQTASFAQVTPILEAGSRVVAEVKADWWPKRGSM
                     QFKVFQIRSVGLGELMARLEALRNILAAEGIFDIDRKKPLPFLPRRIGLICGKNSDAM
                     HDVIKNAKRRWPDIEFVVREVAVQGTTAAKAVSTALSELDAIDDVDVIVVARGGGSFE
                     DLLPFSDEKLIRLVASLETPIVSAIGHEENKPLLDYVADFRASTPTDAARKIVPDVRE
                     ELADLLTARMQLNYLVENKINTLRHQLELVLSRPALAHPGELITQRLEVNHQKRQMIY
                     NLVKNKVDLAFAGLHGVQTTLRALSPQGTLDRGYAIVRNVSGQVISTAELAKTEESLI
                     IKFADGSTKVKPVN"
     gene            2075..>2595
                     /gene="ispH"
                     /locus_tag="AC16Y1_03175"
     CDS             2075..>2595
                     /gene="ispH"
                     /locus_tag="AC16Y1_03175"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR5831498.1"
                     /translation="MPNKKVLLAKPRGYCAGVDRAVVTVEKTLEHYGAPVYVRKEIVH
                     NKFVVSSLEEKGAIFVNETDEIPEGATVVFSAHGVSPAVHQEANERNLKTIDATCPLV
                     TKVHSEAKRFASDDYQILLIGHEGHEEVEGTMGEAPEHMTLVESPEDVETVQVDPNRP
                     VAWLSQTTLSVDET"
BASE COUNT          741 a          555 c          560 g          739 t
ORIGIN      
        1 gtcctaattt aggcagcaca atggtttagt ggccaaaaga agaggcagag aaactgcata
       61 cgacatggtg ctatctatgg gtgcagtttt tgtcgcagtt gcggtaattc ttgtcattgc
      121 ctggcgacca gccaaagatt tcattgctcc agttgattac aacggcgcaa tcgacagtgc
      181 tgtagttaat tcgaattggc caatttatat tccaaaggta attccagaaa attttgaagt
      241 aacctctgca agatttgaac gtgaatctta tggggacagt agagataccc gttggtactt
      301 gggttttgca agcgctgatg aatttatctc gctttggcaa agtgatggcg attttgccca
      361 aataaagtcc gcaacgattc caaatgcgca gtgcaccgaa agcaaagtga tcgccgagca
      421 gtcatggcaa cagtgcactt cgcttgcctc tgcgacaaat ggctatgtcc gaactgacgg
      481 tgaattgatt tacctagttt cggggactgt cgattgggct gaacttgaga cttttatcgc
      541 cagtcttgaa attgcaaagt aaaagctaga gataactaga tatagttttc ttatggctgc
      601 caataaatcg gatttatctt acgaacaagc gaaagcagcg cttgatgaag tagttgcaaa
      661 gttagaagat aaagatttac cgctcgatga catggtgact ttgtgggagc aaggcgaaaa
      721 gttcgccgca atttgtgaag ccaaactaag tgcagccaaa gcaaaacttg atgctcttcg
      781 tccagaagta gatgacgatt agttaactgg tttaactttt gtcgaaccat cagcaaattt
      841 gataattaag gactcttcag tttttgctaa ctctgcagtg ctaattactt ggccagagac
      901 atttctaacg attgcatagc cacgatcaag agttccttgt ggcgaaagag cgcgtaaagt
      961 cgtttgcact ccatgcaatc cagcaaaagc caaatcgact ttgtttttaa ctaagttata
     1021 aatcatttgt cgcttctggt gattaacttc aagtcgttga gttattaatt caccgggatg
     1081 agccaatgca ggcctactta ataccagttc taattgatgg cgaagagtat tgattttatt
     1141 ttcgactaaa taatttaact gcattcgagc agttagtagg tctgctaatt cttcgcgaac
     1201 atcaggcaca atttttcggg ctgcatccgt tggcgtagaa gctcgaaaat ctgcaacata
     1261 atcgagtagc ggtttatttt cttcgtggcc gatggctgaa actattggcg tttctaaact
     1321 agccactaac ctaattaatt tttcatcact aaatggaagt aaatcttcaa aagaaccgcc
     1381 accgcgagcc accacaatta catctacatc atcaatcgca tcaagttcgc ttagtgcagt
     1441 cgacacagct tttgctgctg ttgtgccctg gactgctact tctctaacaa cgaattcgat
     1501 atccggccag cgacgtttag catttttgat aacgtcatgc attgcatcgc tattttttcc
     1561 gcagattaaa ccaattcttc tgggtaaaaa cggcaaaggt tttttgcggt caatatcaaa
     1621 aattccttcg gcagccagga tgttgcggag ggcttcaagt ctggccatta actcgccaag
     1681 cccgacggat cgaatttgaa aaactttaaa ctgcattgac ccgcgttttg gccaccagtc
     1741 tgcttttact tcagctacga cccgtgatcc agcttcgaga atcggagtta cttgcgcaaa
     1801 agatgctgtc tgcatgtaca tctttaatac ttcttcactt tgcacatcgc gcaaagttaa
     1861 gtaagtcaga cctctacctg ttttaacttc aattaactga ccttcaaccc aaacactgcc
     1921 caggcgacca atccaatcgc ttaccaatgc acttacttga tgaacggcaa aaggtgactc
     1981 ggctgaaatg cctagtttga actcatcaga ggctgacacc tttctattct gccttgaact
     2041 ggcactattt aatgggtggg ttacgattag atttgtgcct aataaaaaag tcttactcgc
     2101 aaaacctcgt ggatactgtg ccggtgttga tcgagcagtt gtaacagttg aaaagacttt
     2161 ggaacattat ggcgctccgg tctatgtgcg aaaagaaatt gttcataaca aattcgtagt
     2221 ttcttcactc gaagaaaaag gtgccatctt cgtaaatgaa acagacgaaa tacccgaagg
     2281 tgcaacggtt gtattttctg ctcatggtgt ttcaccggca gttcatcaag aagcaaatga
     2341 acgaaatttg aaaaccattg atgccacgtg tccgctagta acaaaagttc attctgaagc
     2401 taaacgattt gcaagtgatg attatcaaat tctgctaatc ggccatgaag gccatgaaga
     2461 agttgaaggc accatgggcg aagctcccga gcatatgact ttggttgaaa gtcccgagga
     2521 tgttgaaacc gtacaagttg atccaaatcg cccggttgcc tggctttctc aaaccactct
     2581 ttcagttgat gaaac
//
LOCUS       DBNHVN010000166         4402 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_32329, whole genome shotgun sequence.
ACCESSION   DBNHVN010000166 DBNHVN010000000
VERSION     DBNHVN010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_32329"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="AC16Y1_03180"
     CDS             complement(<1..284)
                     /locus_tag="AC16Y1_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831499.1"
                     /translation="MGQHSAPRHRPARTAADARFRLARTFKPRMSKTQATITILFLLL
                     GFTTTSAIIDNEADALLSGTRQSDLVGVLDNLAQREARLRQELISLENDR"
     gene            complement(285..617)
                     /locus_tag="AC16Y1_03185"
     CDS             complement(285..617)
                     /locus_tag="AC16Y1_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HIR5831500.1"
                     /translation="MIPAIGLLLGVVLGLILQPTIPLWLQPYLPIAVVAALDAVFGAF
                     RSILDGIFNDRIFVISFLSNVVVAALIVLLGDQLGVGAQMSTGVVVVLSMRIFANVAA
                     IRRHLLKA"
     gene            complement(614..1396)
                     /locus_tag="AC16Y1_03190"
     CDS             complement(614..1396)
                     /locus_tag="AC16Y1_03190"
                     /inference="COORDINATES: protein motif:HMM:NF017734.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HIR5831501.1"
                     /translation="MTAQLDALLADAIASDFEAGQERKTGTTPIIATIAGATIVTFMI
                     GVAIADSRSNSSVNQSTKTELISRIDEADKRVAALEDQVSQANRDLQAAEQANLAGTS
                     LGSQAQIRLDQLRIAAGFTEVTGLGVSVTLDDAITDELIPDAALQSGRVVDSDLQMVV
                     NGLWQAGATAISINDQRLTGASAIRSAGQAILVDYRPLVRPYKVVAIAKNADALAGRF
                     RENQGGLLLEQLELRFGVVWELETIGQVTLPASTNQNIGGTP"
     gene            complement(1393..1941)
                     /gene="clsA"
                     /locus_tag="AC16Y1_03195"
     CDS             complement(1393..1941)
                     /gene="clsA"
                     /locus_tag="AC16Y1_03195"
                     /EC_number="2.7.8.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006540366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_function: GO:0043337 - cardiolipin synthase
                     (CMP-forming) activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046474 - glycerophospholipid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsA"
                     /protein_id="HIR5831502.1"
                     /translation="MNVWTIPNALSFLRLLGVPLFFWLIVGPQRDGLALLVLVVSGAT
                     DWLDGFLARKLNQFSRLGELLDPLADRLYVFAAIVALYLRDVLPAWVLIALVVRDLSL
                     TFLLIYLKRFGITGLPVHFVGKAATMNLLYALPLLLLSTFTGVIGEVAHVVGWAFLIW
                     GIAMYWYGALLYFDQARKLGLK"
     gene            2046..2119
                     /locus_tag="AC16Y1_03200"
     tRNA            2046..2119
                     /locus_tag="AC16Y1_03200"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2080..2082,aa:Leu,seq:caa)
     gene            complement(2122..2520)
                     /gene="nusB"
                     /locus_tag="AC16Y1_03205"
     CDS             complement(2122..2520)
                     /gene="nusB"
                     /locus_tag="AC16Y1_03205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR5831503.1"
                     /translation="MRTRTKARQRAVEAVFEAEQRNVSVSDVFERNPDVNEYAITIAN
                     SVWSNLSRIDELIETYAKDWQVARMPAVDRAIIRIAVGELLFEKKLDTGVIIAEAMEI
                     ARNLSSEESVKFINGVLSNLGTVRETLSSW"
     gene            complement(2520..3083)
                     /gene="efp"
                     /locus_tag="AC16Y1_03210"
     CDS             complement(2520..3083)
                     /gene="efp"
                     /locus_tag="AC16Y1_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011774957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR5831504.1"
                     /translation="MATSNDLKNGLVLKIDGQLWTVIEFQHVKPGKGPAFVRTKLKNV
                     ISGKVVDKTFNAGVSVETADVDKRDMQYLYRDGDNWVFMDLKDFDQYSLSNEIVGDSA
                     QWMLENQEVIIAIYENAPIYLEMPPNVELTITYTEPGLQGDRSSAGSKPATLETGAEI
                     KVPLFVENGTKVKVDTRDGSYLGRVNE"
     gene            complement(3175..3609)
                     /gene="aroQ"
                     /locus_tag="AC16Y1_03215"
     CDS             complement(3175..3609)
                     /gene="aroQ"
                     /locus_tag="AC16Y1_03215"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR5831505.1"
                     /translation="MTKILILNGPNLDKLGTREPDVYGSLTLADLAKACTAKATELGL
                     EVDFRQSNSETELIEWLHLAAAESYPVIFNPAAFTHYSVAIRDAAALLEAPLIEVHIS
                     NPLAREEFRHTSLISGIAKGTISGFGLNSYLLALSAIADELK"
     gene            complement(3612..>4402)
                     /locus_tag="AC16Y1_03220"
     CDS             complement(3612..>4402)
                     /locus_tag="AC16Y1_03220"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018836725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HIR5831506.1"
                     /translation="GGGATTDLAGFVAATWLRGIKLINIPTTLLGMVDAAIGGKTGIN
                     TDEGKNLVGSFHSPAAVFCDLNALSTMAEHDYVSGLAEVIKAGFISDPKILELIESDP
                     NGAKSPNWQHTEEIIARSVQVKADVVQADLRESLQTQVGREVLNYGHTLGHAIERYEK
                     YSWRHGAAVSVGMMYVAHLANLAGQLSDSVVDRHQRVLDAVGLPTRYKAGAWTELLAA
                     MKIDKKTRGDLLRFVILKDIAKPALLEGPDPALLVAAYQEIVQG"
BASE COUNT         1244 a         1073 c          882 g         1203 t
ORIGIN      
        1 tcacgatcat tttctaaact gataagttct tggcgcaacc tggcttcacg ttgggctaaa
       61 ttatccagca ctcccactaa atcgctttgt ctggtgcctg agaggagtgc atctgcttca
      121 ttatcaatga ttgcagatgt cgtcgtaaat cctaaaagta gaaaaagaat cgtaattgtt
      181 gcttgagtct tagacattct gggtttaaaa gttcgagcta atcgaaaacg agcgtcagcc
      241 gctgtccgtg ccggacgatg tctaggtgcg ctatgttgtc ccatttatgc cttaagtaag
      301 tgacgacgaa ttgctgcgac gttagcgaaa attcgcatcg ataacaccac tactacacct
      361 gtcgacattt gagcgcctac accgagttga tctccgagta acacaatcag agcagcaaca
      421 accacattag ataggaatga aataacaaat attcgatcat tgaaaattcc atcaagaatt
      481 gatcgaaacg ctccaaatac tgcatcaagt gctgcgacta cggcaatcgg taaatatggt
      541 tgcaaccata gcgggattgt cggttgcaat ataagtccga gcacgacgcc taaaagaagg
      601 ccaatagccg gaatcaaggt gtgcctccta tgttttggtt agtggatgcg ggcaacgtga
      661 cttgcccgat agtttccaat tcccagacta cgccgaaacg aagttctaac tgctcaagca
      721 acagtccgcc ctgattttct ctaaaacgac cggcaagggc atctgcattc ttagcgatag
      781 ccacaacttt gtaaggcctg accagaggtc ggtagtcgac cagaatcgct tgccccgctg
      841 atctgattgc agaagcgcca gttaatcgtt gatcatttat tgaaattgca gtggcaccgg
      901 cctgccataa gccgttgacc accatctgta agtcactgtc tactacccgg ccggactgta
      961 acgcagcatc agggattaat tcatcagtga ttgcatcatc caaagtgaca ctgacaccca
     1021 aaccagttac ttcggtgaaa ccggcagcta ttcgaagttg atctagtcga atttgagcct
     1081 gagaacctag tgaggtgccg gctaagttag cttgttcagc agcctgcaaa tctcggttag
     1141 cctgcgatac ctgatcttct aatgcagcaa cgcgcttatc tgcttcatct atccgagaaa
     1201 ttaattccgt tttcgtactt tggttaactg atgaattaga tctcgaatct gcaatggcaa
     1261 caccaatcat gaaggtgaca atagttgcgc cggcgatcgt agcaataatt ggtgttgtac
     1321 cagttttacg ttcttgtcca gcttcaaaat ctgaagctat tgcatctgct aacagagcat
     1381 ccaattgagc ggtcacttta aacccagctt ccgggcttga tcaaaataga gaagtgctcc
     1441 gtaccaatac atcgctatac cccatattaa aaatgcccaa ccaactacat gagcgacttc
     1501 cccaattaca cccgtgaatg tagaaagtaa gagtagcggc aaggcataaa gcaaattcat
     1561 cgtcgcagct ttaccgacaa aatgaactgg aagcccagtt atcccgaatc gttttaagta
     1621 aataagtaaa aacgtcaaag ataaatcacg aactaccagg gcaataagta cccaagccgg
     1681 taaaacatca cgaaggtata gcgcaacaat tgccgcaaaa acgtataaac gatctgctaa
     1741 cggatctagt aactcaccaa gtcgactaaa ttggtttagt tttcgagcta aaaagccatc
     1801 taaccaatct gttgcgccgc ttacgactag aactaacaaa gctagaccgt ctcgctgtgg
     1861 acctacgatt agccaaaaga aaagtggaac accgagcaga cgtaaaaaac tcaacgcgtt
     1921 tgggattgtc cagacgttaa tttcttacct actttcaatc gtaactttca ctcgtaacga
     1981 caatttggtc actattaagc ctacggttcg atttggtaaa taactccgat aatctagtca
     2041 ggcacgccct ggtactccaa ctggcagaga gcgcggtctc aaacaccgtt cagtgtgggt
     2101 tcgaatccca cccagggcac gttaccaact tgataaagtt tcccgaactg tccctagatt
     2161 cgacagcact ccattaataa atttcacaga ttcttcagag cttaaattcc gggcaatttc
     2221 catggcttct gcgatgatca cgccggtgtc taacttcttt tcaaagagta attccccgac
     2281 agcaattcga atgattgctc gatcaactgc aggcatacgc gctacttgcc aatctttagc
     2341 gtaggtttcg attagttcat caattctgga taaattcgac cagactgaat tagcaatagt
     2401 tattgcgtat tcattaacgt cggggttacg ctcaaaaaca tcactaacag atacatttct
     2461 ttgctcggct tcaaatacag cttcaactgc acgctgcctt gcttttgttc gagttcgcac
     2521 tactcgttaa cacgaccgag gtaagagcca tcgcgagtat caacttttac ttttgtgcca
     2581 ttttcaacaa aaagcgggac tttaatttca gcgccagtct cgagcgttgc tggttttgaa
     2641 ccagcgctcg atcggtcacc ctgtagacct ggttctgtat aagtgatagt cagttcaacg
     2701 tttggcggca tttccaaata aatcggagcg ttctcataaa tagcgattat tacttcttga
     2761 ttttcgagca tccattgggc gctatcccca actatttcgt tagacaatga atactgatca
     2821 aaatccttta ggtccatgaa aacccaatta tcgccatcgc gatataaata ctgcatgtcg
     2881 cgtttgtcga catcagctgt ttctaccgag acaccagcgt tgaaggtttt gtctacaact
     2941 ttgccggata taacattttt aagttttgtt ctgacaaacg ctggaccttt accgggttta
     3001 acgtgttgaa attcaattac tgtccagagt tgaccatcaa tttttagtac tagaccattc
     3061 tttaagtcat tcgaagttgc cacgtttacc ctttgttatg tcaaaaatag ttcatctaga
     3121 tcgtattgga agatctataa ataacgaaaa ctatcaattt cgtcgattgt ggtgttactt
     3181 caactcgtct gcgattgcgc tcaacgccag taaataacta tttagtccaa aaccagaaat
     3241 agttcctttg gctatccctg aaattagcga agtgtgtcga aattcttcac gtgccagagg
     3301 gttacttatg tgaacctcga tcaacggagc ctctaataac gccgctgcat ctctaattgc
     3361 tactgagtaa tgcgtgaaag cagcaggatt gaatatgacc gggtaacttt ccgctgcagc
     3421 taaatgcaac cactctatta actcggtctc agaatttgat tgtctgaaat cgacttctaa
     3481 acccaattct gttgccttcg ccgtacaagc tttcgctaag tctgcaagag ttagcgaccc
     3541 gtatacatct ggttcgcggg ttcccaactt gtctaaattt ggtccgttga gaatcaggat
     3601 tttagtcata cttaaccctg cactatctct tgataagcag caactagcaa cgctggatca
     3661 ggaccttcta atagcgctgg cttagcaata tctttcaaaa taacaaatcg cagcaagtcg
     3721 ccgcgagtct ttttatcgat cttcattgcg gcaagaagtt cagtccaagc cccagcctta
     3781 tatcgagttg gtaatccgac cgcgtcaagt actctctgat gtcgatcaac cacggagtca
     3841 ctcaactgac cagctagatt tgccaagtgc gcaacgtaca tcattcccac actcacagca
     3901 gctccgtgtc gccaagaata cttttcgtag cgctcaatcg cgtgacctag tgtgtgaccg
     3961 taatttagta cttcccgacc tacttgagtt tgcaaactct ctcgaagatc cgcctgaaca
     4021 acgtctgctt taacttgtac tgaacgggca ataatttctt cggtgtgttg ccaattcgga
     4081 cttttagcac cgtttggatc cgactcaatc aattccaata tcttcggatc gctgataaag
     4141 ccagccttaa tgacctcagc taaaccactt acataatcgt gttcagccat ggtggatagc
     4201 gcattcaagt cacaaaatac agcagcaggc gaatggaaac ttccaactaa attcttacct
     4261 tcatcggtgt tgatcccagt cttaccacca atggcagcat caaccattcc taagagggta
     4321 gttggaatat tgatcaactt aatcccgcgt aaccaagtcg cagctacaaa acctgctaag
     4381 tctgttgttg ctccaccacc ga
//
LOCUS       DBNHVN010000167         4053 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_328238, whole genome shotgun sequence.
ACCESSION   DBNHVN010000167 DBNHVN010000000
VERSION     DBNHVN010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_328238"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1743
                     /gene="pruA"
                     /locus_tag="AC16Y1_03225"
     CDS             118..1743
                     /gene="pruA"
                     /locus_tag="AC16Y1_03225"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - L-glutamate gamma-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - L-proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HIR5831507.1"
                     /translation="MDAITSVPAPINEKVLNYEPGSPERTALDAALKDTIASTPELTN
                     VIGGVHKMASGDKFEVRSPHDRAQVLGISANSTKEDAVAAIKAAKAAANGWRDLPFDE
                     RAAVILRAADLLAGPWRYKVLASTNWGQSKNAFQAEIDAACELIDFWRFNVHFARQIL
                     QEQPISSPGVWNRSDYRPLEGFVYAITPFNFTAIAGNLPTAPALMGNTVVWKPAPTQM
                     HSAYLLMQLLEAAGLPPGVINLVAGDGIAVSDVALADPDLAGIHFTGSTGVFQHLWKT
                     VGNNIANYRSYPRLVGETGGKDFVFAHPSADPGILETALIRGAFEYQGQKCSAASRAY
                     VPRSVWEKMGDHFLNEVENIKLGHASDHTNFMTAVIDEKAFDRVSGAIDRARADKSVK
                     VAAGGTYDKSDGFFIRPTVLVADDPTTEFFTTEYFGPVLAIYVYEDAKWREVMQQMEG
                     IAQYALTGAVIAQDRTAINEVMHTLRFAAGNFYINDKPTGAVVGQQPFGGGRASGTND
                     KAGAPLNLMRWVSARSIKETLVPPTNYRYPFMK"
     gene            complement(1754..2767)
                     /locus_tag="AC16Y1_03230"
     CDS             complement(1754..2767)
                     /locus_tag="AC16Y1_03230"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR5831508.1"
                     /translation="MTALAELTTIRVGGTPSLLTIASTQDEIIAEVTAADDANEELLI
                     LGGGSNLVVADDFNGRVLKIASSGYENDSTVCAGAWVTVQAGHNWDEFVADCVKNGWL
                     GVEALAGIPGTVGATPIQNVGAYGQQVSETIAQVRAWNRETKKIETIFAANCEFGYRT
                     SVFKRNPNRWVILSVTFQLPLGDLASPIKYNELAVYFDREIGNRISAQELRAGVLELR
                     AGKGMLLNSEDHDTWSVGSFFMNPRVEQPPADAPAWKEADGRYKVSAAWLIEQAGFGK
                     GFGLSDRATLSTKHTLAITNRGNATSDDIVQIANHVRQGVKAKFGIELQLEARLVGCS
                     LVN"
     gene            complement(2784..3206)
                     /locus_tag="AC16Y1_03235"
     CDS             complement(2784..3206)
                     /locus_tag="AC16Y1_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019852768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR5831509.1"
                     /translation="MSVTFEQIKVGDELPSFEINFTRSQLAAYASASGDMNPIHLDEA
                     VAKSVGLPDVISHGMLTMGRAIRVVTDWVEDPTMVIDYSVRMTRPVVVPNTETGTLVS
                     FSAKVSEVNPDRTVRIDLTAVCEGTTVLGQAKALVKFK"
     gene            complement(3206..3643)
                     /locus_tag="AC16Y1_03240"
     CDS             complement(3206..3643)
                     /locus_tag="AC16Y1_03240"
                     /inference="COORDINATES: protein motif:HMM:NF024844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR5831510.1"
                     /translation="MNRDMIGFVYPDSKPVLVTQEHIAAFANAIGDSNPIYFDETFAQ
                     KFGHQTVCAPPTFPISVCMKAMEDSFHDPRLNMDYSRIVHSDQRFEYVRPIQVGDELV
                     VRTVVEEIKALGNNDMATFRTEVYSKNELVVTGWSKLVVRGVE"
     gene            complement(3687..3851)
                     /gene="rpmG"
                     /locus_tag="AC16Y1_03245"
     CDS             complement(3687..3851)
                     /gene="rpmG"
                     /locus_tag="AC16Y1_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR5831511.1"
                     /translation="MARNDVRPKITLACEDCKDRNYISRKNRRNDPDRLEIKKHCPKC
                     NKHTVHKETR"
     gene            complement(3901..3974)
                     /locus_tag="AC16Y1_03250"
     tRNA            complement(3901..3974)
                     /locus_tag="AC16Y1_03250"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3938..3940),aa:Met,seq:cat)
BASE COUNT         1102 a          956 c          873 g         1122 t
ORIGIN      
        1 ttagtaactc caaatcggca actttgcccg gtaaataaac cctcacctat ttattgtggc
       61 cgttcttttg tccagggtca gtgtggacac ggagagtaaa agtgtcagta tttatacatg
      121 gatgcaatca cctcagtacc ggccccaata aatgaaaaag ttttaaatta tgagccaggt
      181 tcgcctgagc gcacagcgtt agatgcagcg ctaaaagaca ctattgcgag tacgcctgaa
      241 cttactaatg tcataggcgg cgtgcacaaa atggcaagtg gcgacaaatt tgaagttcgc
      301 tctccacatg atcgggcaca agttttaggt atcagcgcca actcaacaaa agaagatgct
      361 gtcgctgcaa tcaaagcagc taaagctgct gcaaatggct ggcgtgatct gccattcgat
      421 gaacgtgctg cagttatttt gcgggctgca gatttacttg caggtccatg gcgctacaag
      481 gttcttgctt cgacaaactg gggtcaatca aaaaatgcat tccaagcaga aattgatgca
      541 gcgtgcgaac taattgattt ctggcgcttt aatgtgcact tcgctcgcca aattttgcaa
      601 gaacaaccca tttcttcacc aggagtttgg aaccgtagcg actaccgccc acttgagggt
      661 ttcgtttatg caatcacacc gtttaatttc acggcgatcg caggcaactt acctactgca
      721 ccagcgttga tgggtaacac agtcgtttgg aaaccagcac caacacaaat gcattctgca
      781 taccttttga tgcagctgtt ggaagcagct ggattaccac cgggtgtaat taacttagtt
      841 gctggtgatg gcattgcagt atccgatgtc gcgctagctg atccagattt agctggaata
      901 catttcactg gatccaccgg agttttccaa cacctttgga aaactgtcgg aaacaacatt
      961 gctaattacc gcagctaccc acgtttagtc ggtgaaacgg gcggtaaaga ctttgtgttt
     1021 gctcaccctt cagcagatcc aggaatactc gaaacagcgc taatccgtgg cgcctttgaa
     1081 taccaaggtc aaaaatgttc agccgcatca cgtgcttatg tgccacgcag tgtttgggaa
     1141 aaaatgggtg accacttctt gaatgaagtt gaaaacatta agctgggcca cgccagcgat
     1201 cacaccaatt tcatgacagc tgttattgat gaaaaagcat ttgatcgcgt tagtggagca
     1261 attgaccgag cccgcgccga caagtcggta aaggtggctg ctggtggaac ttatgacaag
     1321 agtgacggtt tcttcatcag gccaacagtg ctcgttgccg atgatccaac aactgaattc
     1381 tttactaccg aatattttgg cccagtgctt gcgatctacg tttatgagga tgcaaagtgg
     1441 cgcgaagtaa tgcagcagat ggaaggtatt gcacaatacg cgttaactgg tgccgtgatt
     1501 gctcaagatc gcaccgcgat taatgaagta atgcatactc tgcgctttgc tgcgggtaat
     1561 ttctacatca acgataaacc aacaggtgcc gtcgtcggtc aacaaccttt cggtggtggc
     1621 cgagcttctg gtactaacga taaagctggt gcgccgctga acttaatgcg ttgggttagt
     1681 gcccgcagca ttaaagaaac tttggttccg ccgacaaatt atcgttatcc gtttatgaaa
     1741 taacaagaaa gctttaatta actagcgaac acccaactag acgagcttca agctgtaact
     1801 caataccgaa tttagctttt acaccttgtc ttacgtggtt tgctatctgc acgatgtcat
     1861 cacttgttgc gttacctctg ttggtaattg caagtgtgtg ttttgttgaa agtgttgctc
     1921 gatcggacaa accaaaacct ttaccaaagc cagcctgttc aattaaccat gcggcgctaa
     1981 ctttgtagcg accatcggct tccttccagg ctggtgcatc ggcaggtggt tgttcaacgc
     2041 gtgggttcat gaaaaatgac ccaacactcc aggtgtcgtg atcttcggag ttaagtaaca
     2101 tgccttttcc agcgcgaagt tcaagtacac cagcgcgaag ttcttgcgca ctaatacgat
     2161 tgccaatctc gcgatcaaaa taaacggcaa gctcgttgta cttaattggg ctagctaagt
     2221 cgcccagtgg caactgaaaa gtcacactta atataaccca acgatttggg tttcgtttaa
     2281 atactgaagt tcgataacca aattcacagt tggctgcaaa aattgtttcg atttttttag
     2341 tttcgcgatt ccatgcccga acttgcgcaa tagtttcgct aacttgttga ccgtatgccc
     2401 caacattttg gatgggtgtg gcacctaccg tgccaggtat tccggctaac gcctcaacac
     2461 ctaaccagcc attttttaca caatctgcaa caaactcatc ccaattatga ccagcttgga
     2521 ctgtaaccca agcaccagcg caaacagttg agtcattttc atatccactt gaagcaatct
     2581 ttaatacccg gccattgaaa tcatcagcca caactaaatt tgagccaccg ccaagaataa
     2641 gtaattcttc gtttgcatca tcagcggcgg taacttcagc aattatctcg tcctgagtac
     2701 tggcaatggt tagcaaactt ggcgttccgc caactcgaat tgtggttagt tcagccaaag
     2761 cagtcattac ttaaaccgta tatctatttg aacttaacta aggcttttgc ttggccaaga
     2821 actgtggtgc cctcacaaac tgcagttagg tcaattcgaa ctgtccgatc agggtttact
     2881 tcgcttactt ttgcgctaaa tgagactaaa gttcctgttt cagtatttgg tacaaccact
     2941 ggacgagtca ttcgcacgct gtaatcgatc accatagtcg ggtcttcaac ccaatctgta
     3001 actactcgaa ttgctcgtcc catagttaac atgccgtgtg aaataacatc tggtaacccc
     3061 actgactttg ccactgcttc gtcaaggtga attggattca tgtcaccaga agcacttgcg
     3121 tatgcagcaa gttggctgcg agtgaaattt atttcaaaac ttggcaactc atcgccaact
     3181 ttaatttgtt caaaagttac tgacattact caacacctcg aacaacaagt tttgaccagc
     3241 cagttactac taactcgttt tttgaataaa cctcagttcg aaaagttgcc atgtcattat
     3301 tgccaagggc cttaatttct tcaacaactg tgcggaccac taattcgtcg ccaacttgaa
     3361 ttggcctaac gtattcaaat ctttgatcac tatgcacaat gcgtgagtaa tccatattta
     3421 aacgtggatc gtggaaagaa tcttccatgg ctttcataca cactgatatt ggaaatgttg
     3481 gcggcgcaca aactgtttgg tgcccaaatt tttgcgcaaa agtttcgtca aaataaattg
     3541 ggttggaatc acctatcgca ttagcaaaag ctgcaatatg ttcttgggtc accaaaacag
     3601 gctttgaatc tggatagaca aagccgatca tgtcgcggtt catacccatg gcaaaacctt
     3661 aatttcaaaa attaatttga aagtaattag cgagtttctt tgtgcacagt gtgcttgttg
     3721 cacttagggc aatgcttttt gatttcaaga cgatcaggat catttcgacg attcttacgt
     3781 gaaatgtagt tccgatcctt gcagtcttcg catgccaaag tgatctttgg acgaacgtcg
     3841 ttacgtgcca ctttcttgcc tttcagtttt ggcaaagcaa tgctttacca gccaacttcg
     3901 taccgagggc cggacttgaa ccggcgacac aacgattatg agccgtttgc tctaccaagc
     3961 tgagctaccc cggtgaatag ttaatcgtta gattaacttt cgagccccct tgcggaatcg
     4021 aaccgcagac cttttcctta ccatggaaac gct
//
LOCUS       DBNHVN010000168         4006 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_32666, whole genome shotgun sequence.
ACCESSION   DBNHVN010000168 DBNHVN010000000
VERSION     DBNHVN010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_32666"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="AC16Y1_03255"
     CDS             <1..1258
                     /locus_tag="AC16Y1_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5831512.1"
                     /translation="FERLMKLREDLANKDEELRTLRARWDQEKAGLDRVGEIKVRIDE
                     LRSEAERALRDGDLERASRIQYAELPELETELKTVNELANQRSAMVKEEVTADDVAEV
                     VAAWTGIPAGRLLEGETQKLLHMEKLIGTRVIGQAKAVSAVADAVRRSRAGISDPNRP
                     TGSFLFLGPTGVGKTEMAKALAEFLFDDDRAMVRIDMSEYGEKHSVSRLVGAPPGYVG
                     YEEGGQLTEAVRRRPYSVVLLDEVEKAHPDVFNILLQVFDDGRLTDGQGRTVDFRNVI
                     LILTSNLGSQFLIDPILSDEQKRSSVMEIVRSSFKPEFLNRLDDIVMFEPLGTNELTS
                     IVDMQIGAIQKRLDEKRMTLAVSQAAKDWLVLNGFDPIYGARPLRRLVQTAIGDQLAK
                     AILAGTISDGETVKVDVAGETLSVSS"
     gene            1293..2042
                     /locus_tag="AC16Y1_03260"
     CDS             1293..2042
                     /locus_tag="AC16Y1_03260"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5831513.1"
                     /translation="MGTALITGATAGIGKSFAEQLANRGYNLVLVARDRSRLDELAIQ
                     LQQKYQTKVEVLVADVSNQTDLAEVACRAAAEDIDLVVNNAGFGIKQTFVGGRIEDEQ
                     NLLDVLVTAVMRVTHAALPGQVKRNRGGVINVSSVAGWLSSGTYSSAKAWVTTFSEAL
                     ATQLKKQDVHVMALCPGYTRTEFHQRAEMKVETIPNWMWLDVDRVVQKALKDFAANKP
                     VSVPGLQYKFLSFIAQYLPRPIVRIISNQSR"
     gene            complement(2044..2472)
                     /locus_tag="AC16Y1_03265"
     CDS             complement(2044..2472)
                     /locus_tag="AC16Y1_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831514.1"
                     /translation="MATVAFLPTVDGINPFAQSIQLKVSPFVVSPPTTNVRWVAHVCG
                     QLATTAEELILVFVGEESVHAPAVGFSRRSLHRPAVSYVFIDPVMPKIGGDYSDWPDA
                     PVTVVLTDAASDTAKEASLQAKLRGWIVTTESVQSVLASF"
     gene            2542..3075
                     /gene="pyrE"
                     /locus_tag="AC16Y1_03270"
     CDS             2542..3075
                     /gene="pyrE"
                     /locus_tag="AC16Y1_03270"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR5831515.1"
                     /translation="MSDRELLKAQILEKAVVHGKVILSSGKEADCYVDLRRITLDAVA
                     APIVGKVMLELTKDLDFDAVGGLTLGADPVATAMLHAASAQGRKLDAFVVRKAEKAHG
                     LQRRIEGPDVKGRRVLAVEDTSTTGGSVLTAVEALQEAGATVVGVAVIVERAAGPAIE
                     AKGLPYLYAYNLVDLGL"
     gene            3160..>4006
                     /gene="fbaA"
                     /locus_tag="AC16Y1_03275"
     CDS             3160..>4006
                     /gene="fbaA"
                     /locus_tag="AC16Y1_03275"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIR5831516.1"
                     /translation="MPIASPDVYNEMLDRAKAGAFAYPAINCTSSQTIVAAIRGFAEA
                     ESDGIVQVSWGGAEFASGQPVKDMVAGAAALAEFTHSIAKSYNVNIALHTDHCPAEKL
                     DGFMRPLVQISADRVKNGQQPLFQSHMWDGSAVSLTENLKIADEMLDKCVAAKTILEI
                     EIGVVGGEEDGIEAKHDAKLFSTPEDALAMAEKLGVGERGRYLVAATFGNVHGVYKPG
                     NVVLTPSILKDLQEAVQKKHNTKEKPLDLVFHGGSGSLLSEIREALDYGVIKMNIDTD
                     TQYAFT"
BASE COUNT         1151 a          803 c          986 g         1066 t
ORIGIN      
        1 ttttgaacgg ctgatgaagt tgcgcgagga cttagcaaat aaagacgaag aacttcgcac
       61 tctgcgcgcc agatgggatc aagagaaagc tggcttagat cgcgttggtg aaatcaaagt
      121 tcgcattgat gaactgcgct cagaagctga acgtgcatta cgtgatggcg acttagagcg
      181 agcaagtcgt attcagtatg ccgaattgcc tgaattagaa acagaactta aaacggttaa
      241 cgaactagcc aatcaacgct cagccatggt taaagaagag gtaactgcag atgatgttgc
      301 tgaagtagtt gctgcttgga ctggtattcc agctggcaga ttacttgaag gtgaaacaca
      361 aaaactactg catatggaaa agctaattgg cactcgagtt attggccaag cgaaagcagt
      421 tagtgctgtt gctgatgctg tgcgccgatc tcgagctgga atctctgacc caaatcgacc
      481 aactggttct tttttattct tgggtccaac tggcgttggc aaaacagaga tggccaaagc
      541 ccttgctgaa tttttattcg atgatgatcg agccatggtt cgaatcgaca tgagtgaata
      601 tggtgaaaaa cattctgtat cgcgcttagt cggcgcgccg cctggatacg ttggttacga
      661 agaaggtggc cagttaaccg aagctgttcg tcgccgacct tacagtgttg ttctgttgga
      721 tgaggttgaa aaagcacatc ctgatgtatt caacatcttg ctccaagtct ttgatgatgg
      781 ccgattaact gatggtcaag gtcggacagt tgactttaga aatgtgattc ttattttgac
      841 ctcaaattta ggttcacaat ttttaattga tccaattttg tctgatgagc aaaaacgttc
      901 tagcgtgatg gagattgtgc gatcttcgtt taagccagaa tttttgaatc gcctagatga
      961 catcgttatg tttgagccac ttggcaccaa cgagttaact tcaatcgttg acatgcaaat
     1021 cggtgcaatt caaaagcgac tcgatgagaa gcgaatgact ttagcggtaa gccaagctgc
     1081 taaagattgg ttagtgctga atggattcga tccaatctat ggggcaaggc cgcttcgacg
     1141 attagttcaa actgccatcg gagatcaact agcaaaagca attttggctg gaaccatcag
     1201 cgatggagaa acagtcaaag ttgatgttgc cggtgaaacg ctctcagtaa gttcctaact
     1261 gtaaatctga agttgtttgc gacaattacc taatgggtac tgctttaata actggcgcaa
     1321 ctgcaggaat tggaaagtca tttgcagagc aacttgctaa tcgtggctat aacttggttt
     1381 tagttgcacg ggatagatca cgacttgatg agttagcaat tcagctacaa cagaaatatc
     1441 aaacaaaagt tgaagtattg gttgcagatg tttctaatca aacggatttg gcagaagttg
     1501 catgtcgggc ggcagccgaa gacattgatc tagttgttaa taatgctggg tttggaatta
     1561 aacaaacctt tgttggtgga cgcattgaag atgaacaaaa ccttttagat gttctagtta
     1621 cagcggtcat gcgcgttacc catgcggcac tacctggcca agtaaagcga aatcgtggtg
     1681 gcgtgattaa tgtgagttca gttgcaggtt ggttatcaag tggaacttat agcagcgcaa
     1741 aagcatgggt aactactttt agtgaagcat tagctaccca acttaaaaaa caagatgttc
     1801 atgtgatggc actatgcccg ggatacaccc gaactgaatt tcaccaacgt gctgaaatga
     1861 aagtcgaaac gattccaaat tggatgtggt tagacgttga tcgtgtggtt caaaaagcac
     1921 ttaaagactt tgctgcgaat aaacccgtca gtgtgccagg tttgcagtac aaatttttaa
     1981 gttttatcgc gcagtattta cctaggccga tagttcgaat aatttcaaat caatctaggt
     2041 agtttagaaa cttgctaaaa cgctctggac agattcggta gttacaatcc agccacgaag
     2101 ttttgcttga agtgatgctt ctttagctgt atcacttgct gcatctgtta agacaactgt
     2161 taccggagca tcgggccaat cactgtagtc gccgccaatt ttgggcatta ctggatcaat
     2221 aaacacgtaa ctaactgctg gtctgtgaag cgaacgtcga ctaaatccaa cagcgggagc
     2281 atgcaccgat tcttcgccaa caaaaactaa gatcagctct tcggcagtag tggccaattg
     2341 accacacaca tgtgccaccc atcgaacatt tgttgtaggc ggactaacga caaagggtga
     2401 aacttttaac tggatcgatt gagcgaaagg attaattccg tctacagtcg ggaggaaagc
     2461 caccgttgcc atgtgactac tgtgccacga ttttggttag ttgagcgagt tcgcaagtaa
     2521 tcgtgggttt aggctgtaac catgtcagat cgcgagttac tcaaagcaca gatacttgaa
     2581 aaagcagtag tacatggcaa agttattttg tccagcggca aagaagctga ctgctacgtt
     2641 gacttacgtc ggattacctt agatgcagtt gccgcgccaa ttgttggaaa agtaatgctt
     2701 gagttaacca aagatttaga tttcgatgca gttggcgggt taactctagg tgccgatcca
     2761 gtagcaacag caatgctgca cgctgccagc gcacaaggta gaaaacttga tgcttttgtt
     2821 gttcgcaaag cagaaaaggc acatgggtta caacgccgaa tcgaagggcc agatgtgaaa
     2881 ggtcgtcgag tcctggcagt tgaagacacc agcaccaccg gtggctcagt attaactgcg
     2941 gttgaagcac ttcaagaagc tggtgcgaca gtggttggcg ttgcggtaat tgtggaacgt
     3001 gctgctgggc cagcgataga agctaaaggg ttgccttatt tatacgcata taacttggtt
     3061 gacttaggtc tttagtagcc aattcccaaa ccagtattta accaaaatgg cttccgtgag
     3121 agaatggctt tgcgctaatc cgttttctta ggagtaaaaa tgcctattgc tagtcctgac
     3181 gtttacaacg agatgcttga tcgagcaaag gctggcgctt tcgcatatcc agcaattaac
     3241 tgcacctcat cgcaaacaat tgtcgcggca attcgtgggt ttgccgaagc agaatcagat
     3301 ggcatagttc aggtttcttg gggtggcgca gaattcgcat ctggtcaacc ggttaaagat
     3361 atggttgctg gcgcggcagc attggcagaa tttacacact caatcgcaaa aagctacaac
     3421 gttaacatcg cattgcatac cgatcactgt ccggcagaaa aacttgatgg gtttatgcga
     3481 ccacttgtac agatttcagc tgatcgagtt aaaaatggtc agcagccact tttccagtcg
     3541 cacatgtggg atggatcagc agtttcactc actgaaaatt taaagattgc tgacgagatg
     3601 ttagataagt gcgttgcagc aaaaacaatt ttagaaatcg aaatcggtgt tgttggtggc
     3661 gaagaagacg gtattgaagc aaaacatgat gccaaattat tctcaacacc cgaagacgcg
     3721 cttgcgatgg ccgaaaaact tggtgtgggc gaacgtggtc gctatttagt ggccgccacc
     3781 ttcggcaatg tgcacggtgt ttataagcca ggtaacgtcg tattaactcc gtcaatactt
     3841 aaagatctac aagaagcagt acaaaaaaag cacaatacga aagaaaaacc tctcgatcta
     3901 gttttccatg gcggatctgg ttcactactt tccgaaattc gcgaagcttt ggattacggt
     3961 gtgatcaaaa tgaatatcga tacggatacg cagtatgcgt ttactc
//
LOCUS       DBNHVN010000169         2317 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1741140, whole genome shotgun sequence.
ACCESSION   DBNHVN010000169 DBNHVN010000000
VERSION     DBNHVN010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1741140"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2049)
                     /gene="leuS"
                     /locus_tag="AC16Y1_03280"
     CDS             complement(<1..2049)
                     /gene="leuS"
                     /locus_tag="AC16Y1_03280"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017202347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR5831517.1"
                     /translation="MSTQLPNAEQNQQDPNAIDFNAIQEKWLPIWDELAPFRSGNPDD
                     SRPKKYVLDMFPYPSGDLHMGHAEAYALGDVLARYWVHKGFNVMHPIGWDAFGLPAEN
                     AAIKRNESPAIWTYENIAVQKASMRRYACSFDWDRVLNTCDPEYYKWNQWFFLQMYNR
                     GLAYRKASAVNWCPSCQTVLANEQVVAGLCERCDSVVTKKKLTQWYFKITDYADRLLD
                     DMVQLEGNWPEKVLQMQRNWIGRSQGAEVTFDIEGMDKSVVVYTTRPDTLFGATFFVV
                     AADSELASELASGTQSQKEFEKYLENVKGTSDIDRLATDRPKTGVFLNRYAINPVNGE
                     KLPIWASDYVLADYGTGAIMAVPAHDQRDLDFARAFNLPIRVVVASDTDPAETGVATA
                     DDGAHVNSGPLDGLSKAEAISKMIEILQKNGRGKSAKNYRLRDWLISRQRFWGTPIPI
                     VHCEKCGEVPVPENQLPVELPDATGLDLAPKGTSPLGAAQDWVNVNCPTCGQPAKRDA
                     DTMDTFVDSSWYYMRYLNPKLADQAFDQEQAKKWLPIDQYVGGVTHAILHLLYSRFFT
                     KVMYDMGLVNFTEPFTRLLNQGMVIMDGSAMSKSRGNLVRLSDELNDHGVDAVRLTMV
                     FSGPPEDDVDWADVSPSGSKKFLGRAYRLSADVKSPVDVDFAAGDRTLRKATHRALND
                     A"
     gene            2096..>2317
                     /locus_tag="AC16Y1_03285"
     CDS             2096..>2317
                     /locus_tag="AC16Y1_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR5831518.1"
                     /translation="MTKRGWLLIGALGLIWGTPYLFIRVAVLELSPPVVVFSRLILGA
                     LVLLPLALRHGQGGVLRTHWKGILTFAFIE"
BASE COUNT          597 a          583 c          479 g          658 t
ORIGIN      
        1 agcatcgttt agcgcacgat gtgttgcttt acgtaatgtc cgatcacctg ctgcgaagtc
       61 aacatctacc ggacttttta catcagctga taagcggtag gcacgtccca aaaacttttt
      121 tgatccactt ggcgacacat cagcccagtc cacatcatct tcaggcggtc cagagaaaac
      181 catagttaac cgaacagcat caacgccgtg atcatttagc tcatcactta gccgaactaa
      241 attgccgcgg cttttgctca ttgctgaacc gtccataatt accatgcctt gatttagtaa
      301 tcgagtgaac ggctcggtga aattaactaa gcccatgtca tacataactt tggtaaaaaa
      361 gcgactgtaa agtaaatgca aaattgcgtg cgtaacgcca cctacatatt gatcaattgg
      421 tagccatttt ttggcttgct cctgatcaaa cgcctgatca gctaacttcg gattcaaata
      481 gcgcatgtag taccaagatg aatctacgaa ggtatccata gtgtctgcgt cacgcttagc
      541 tggttggcca caagttggac agttaacatt tacccagtcc tgcgccgcgc ctaacggaga
      601 tgttcctttg ggtgctaaat ccaacccagt cgcatctgga agttcaaccg gcaattgatt
      661 ttccgggact ggaacttccc cacacttttc acaatgcacg attgggattg gcgttcccca
      721 gaacctttgc cgagaaatta accagtcgcg aagacgatag tttttagccg acttacctcg
      781 accatttttc tgcaaaattt caatcatttt gctgattgct tcagccttag agagtccatc
      841 tagcgggcca gaatttacat gcgctccatc atctgcagtt gctactccgg tttctgctgg
      901 gtcagtatca ctggcgacca ctacgcgaat cggtaaatta aaagcacgcg cgaaatctaa
      961 atctcgttga tcgtgagcag gtaccgccat aattgcacca gtgccgtaat ctgcaagtac
     1021 ataatcgcta gcccaaattg gtaacttctc accattaacc ggatttattg cgtagcgatt
     1081 tagaaacact ccagtcttag gacgatcagt tgccaagcga tcaatgtcgc tagttccttt
     1141 gacattctct aagtattttt caaattcttt ttggctctgc gtcccacttg ctagttcgct
     1201 tgctagttct gaatcagcag caacaacaaa aaatgttgcg ccgaataaag tatctggacg
     1261 agttgtgtaa accacaactg acttgtccat gccttctata tcaaaagtga cttcagcacc
     1321 ctggctgcgg ccaatccagt tacgctgcat ttgcagcact ttttctggcc aattgccctc
     1381 gagttgcacc atgtcatcta gtaggcgatc tgcataatca gttatcttga aataccactg
     1441 agttaatttc tttttggtga caaccgaatc gcatcgctcg cacaacccag cgacaacctg
     1501 ttcattagcc aacaccgttt ggcatgacgg acaccagtta actgcggatg cttttcggta
     1561 agccaaacct cggttgtaca tctgcagaaa aaaccattgg ttccatttgt aatattcagg
     1621 atcgcaggta tttaaaaccc gatcccaatc aaaagagcag gcgtatcgac gcatcgatgc
     1681 tttttgcacc gcaatatttt cataagtcca gattgccggg ctttcattac gtttaatcgc
     1741 ggcattttca gctggtaaac caaatgcatc ccagccgatt ggatgcatta cattaaaacc
     1801 tttatgcacc caataacgag caagtacatc accgagtgca taagcttcgg catgccccat
     1861 gtgtaaatca cccgaggggt aaggaaacat atcgagtaca tatttttttg gccgagagtc
     1921 atctgggttt cccgaacgaa atggcgctaa ctcgtcccaa atcggcagcc atttttcttg
     1981 aatggcgtta aagtcaatcg catttgggtc ttgctgattc tgctctgcat tcggcaattg
     2041 cgtactcata atccccaaga ttactggact tttacacctg tctagactcg tgctcatgac
     2101 taaacgcggt tggctattaa ttggggcact tggactaatt tggggaactc cctatttatt
     2161 tattcgggtt gcagtcctag aactttcccc gccggtcgta gtttttagtc gattaattct
     2221 tggcgcgtta gttttattgc cgctagcttt acgccacgga cagggcggcg tccttcgtac
     2281 ccattggaaa ggcatcttga ctttcgcgtt catcgag
//
LOCUS       DBNHVN010000170         1930 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_328546, whole genome shotgun sequence.
ACCESSION   DBNHVN010000170 DBNHVN010000000
VERSION     DBNHVN010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_328546"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="AC16Y1_03290"
     CDS             complement(<1..818)
                     /locus_tag="AC16Y1_03290"
                     /inference="COORDINATES: protein motif:HMM:NF028500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HIR5831519.1"
                     /translation="MTTLNSPTSDIVGAKTATVLESEFGIRTVGDLVRHYPRRYEQRG
                     ALTDLGLLEIGDHVTVQAEVRSFKNLKNKSKAGSRQEVIISDGTGALTLMFFNQHWRE
                     KDLLPGRRGLFSGQISVFNNKRQLTHPEYQLIDETTEVNAADYTEALLPVYPATSKVQ
                     SWTIERAINSALTKLEDLPDPIDSSFRKEHDLLDLTTALKLIHQPANQIELDQAIARL
                     KWDEALVLQLLLAKERKELEMAPAAPRLVKPDGLLANFDQQLPFKLTAGQQAVT"
     gene            complement(815..1714)
                     /locus_tag="AC16Y1_03295"
     CDS             complement(815..1714)
                     /locus_tag="AC16Y1_03295"
                     /inference="COORDINATES: protein motif:HMM:NF014754.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HIR5831520.1"
                     /translation="MPGLQLENLTAPDFVRALGLFKEYLSNYEQLINELNVYPVPDSD
                     TGTNSLLTFSAGLANLTQEQEVSALARSLATGCAKSGLGNSGVILAAYLVGFAEALTD
                     PVNLSVWQNATRSAAISANKSVLHPTQGTMLTIATEVSSVQETTLAEQFFANSTAARL
                     ALVKTTDLLPELKLAGVVDSGAVVLTLFHDAFAKLVTEGNFTDLEIINTKCLAKPDYH
                     GPNHELMFSITLTPENKLKLLSQLEKLGDSISISDIANLAESKEEVRIHIHCDDPDQV
                     ITEAKTFGKVFDLVLINLTLKNL"
     gene            1852..>1930
                     /locus_tag="AC16Y1_03300"
     CDS             1852..>1930
                     /locus_tag="AC16Y1_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014628431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large ribosomal subunit protein bL28"
                     /protein_id="HIR5831521.1"
                     /translation="MAAVCDVCGKGPGFGHNISHSHRRTK"
BASE COUNT          527 a          402 c          411 g          590 t
ORIGIN      
        1 ttagtaaccg cttgctggcc agcggttaat ttaaacggga gttgctgatc gaaatttgcc
       61 agtaacccat caggtttgac caatcgcggc gcagcagggg ccatttctaa ttctttgcgc
      121 tctttagcta aaagcaattg gaggactaat gcctcgtccc acttcagcct ggcaatcgct
      181 tggtctaatt caatttgatt tgcgggctga tggattaact taagtgcggt tgttaaatct
      241 aaaagatcat gttcttttcg aaatgaacta tcgattggat caggtaaatc ttcaagtttt
      301 gtaagcgcac tattgattgc tcgctcaata gtccaggatt gcactttgct tgtggctgga
      361 taaacaggta acaaagcttc ggtgtaatca gcggcattta cttctgttgt ttcatcaatt
      421 aactgatatt cgggatgggt taattgtcgc ttgttattga aaactgaaat ttgtccagaa
      481 aacagcccgc gtcgccctgg cagtaaatct ttttctcgcc aatgttgatt aaaaaacatc
      541 aacgttagtg ctccagtgcc gtcactgatg attacttctt ggcgactacc agccttggac
      601 ttatttttta aatttttaaa actgcgaacc tcagcttgca cggttacatg atcaccgatc
      661 tcaagtaaac ctaaatcagt taaagcgcct cgttgctcat accgtcgtgg gtaatgtctg
      721 actaagtcac caactgtgcg aataccaaat tcactttcga ggacagttgc agtttttgct
      781 ccgacaatgt cagaagttgg cgaatttaac gtggtcatag gttcttgagg gttaggttta
      841 ttagcactaa atcgaaaact tttccaaatg ttttagcctc ggtaatcacc tggtctggat
      901 catcgcaatg aatatgaatc cgaacttctt ctttagactc agctaggttg gcaatatctg
      961 aaattgaaat agagtcacct agtttttcaa gttgtgacaa caacttaagt ttattttctg
     1021 gggtgagtgt tatcgagaac ataagttcat gatttggacc atggtaatcc ggttttgcta
     1081 aacactttgt atttattatt tctaggtcag taaaatttcc ttcagttacc agcttggcaa
     1141 atgcatcatg aaaaagcgtc aacaccactg cccccgaatc aacaacgcca gccaatttga
     1201 gttcaggcag taagtccgtg gttttaacaa gtgctaaccg tgcggcagtt gaatttgcaa
     1261 agaattgttc agctaacgtc gtttcctgca cggaactaac ttctgttgca atagtcaaca
     1321 tggtgccttg tgttggatgt agtacagact tatttgcact gatcgcagcc gatcgggtcg
     1381 cattttgcca aacggataga tttactggat cagttagtgc ttctgcaaag ccaactaaat
     1441 atgctgccaa aataacgcct gagttaccta gacccgattt tgcgcaaccg gtagctagtg
     1501 atcgggccag agctgaaact tcttgttctt gagtaaggtt tgctaaccca gcgctaaaag
     1561 ttaatagcga gttagttccg gtatcggaat ctggaactgg ataaacattt aactcattta
     1621 ttaattgctc ataatttgat agatattctt taaataagcc aagtgcccgc acaaaatcag
     1681 gtgcagtgag attttctaat tgcagcccgg gcacttcttt aggttacctg cattgatttt
     1741 tcgtactaga tcgccgataa ataaggcata aattaacccg aattggaaat tgcctgaaaa
     1801 cacaggtatt gtaaggattc gcaagaattg cgctcaacct taggagtcaa agtggctgct
     1861 gtatgtgatg tctgtggcaa aggcccaggc tttggccaca atatttctca ctcacaccgt
     1921 cgcaccaagc
//
LOCUS       DBNHVN010000171         3758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_328555, whole genome shotgun sequence.
ACCESSION   DBNHVN010000171 DBNHVN010000000
VERSION     DBNHVN010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_328555"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..512
                     /locus_tag="AC16Y1_03305"
     CDS             99..512
                     /locus_tag="AC16Y1_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HIR5831522.1"
                     /translation="MEAQGGSPSGLAGKFGFAEGFVVQEIGFDVDCDKSVSTAARSVV
                     ELVDEAYEDIVDAVILWWREDDGDLVDALVDALTPLADNGVIWLMTPKAGRPGHVEPS
                     DIAEAAPTAGLQATSNINAAPDWQGTRLVAPKARR"
     gene            551..1012
                     /locus_tag="AC16Y1_03310"
     CDS             551..1012
                     /locus_tag="AC16Y1_03310"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017593737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR5831523.1"
                     /translation="MSLEVGKEAPDFTLKNQFGEEVKLSDFRDKKNVVLVFFPMAFTG
                     ICTGELCEIRDNSPVFVSDSVQVLGISCDTSATHKVFAEKENLDYPLLSDFWPHGAVS
                     KMYGSFFEERGFSTRGTFIIDKQGILRWSVVNSPGDARSTDEYKKALAELS"
     gene            complement(1260..1333)
                     /locus_tag="AC16Y1_03315"
     tRNA            complement(1260..1333)
                     /locus_tag="AC16Y1_03315"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1297..1299),aa:Val,seq:tac)
     gene            1445..2224
                     /locus_tag="AC16Y1_03320"
     CDS             1445..2224
                     /locus_tag="AC16Y1_03320"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase family protein"
                     /protein_id="HIR5831524.1"
                     /translation="MSELMPALYIGHGAPPLLDDPVWTTELKQLAASMPTPKAVLIVS
                     AHWESAPLTIGATEFGTPLIYDFGGFDPKYYNLKYETPDSTALANLVKSVLADTQTIS
                     EQPKRGLDHGAYVPLLTMYPEANIPVLQMSIPTHNPIELVKLGQRLAKLREHGVLIIG
                     SGFLTHGLPYLRDFRIEATPPSWSVEFDRWAQEAIGKGDLDVLMNYKNLAPGMPYAHP
                     TVEHLSPIFVTLGAASNSTVAPEVVIDGYWMGLAKTSIQVA"
     gene            2247..3056
                     /locus_tag="AC16Y1_03325"
     CDS             2247..3056
                     /locus_tag="AC16Y1_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HIR5831525.1"
                     /translation="MSLKLTDVIAAMERRYPTATAQEWDAVGLVAGNPDSEISKVLFA
                     VDPSEVVVEQAIQVGAQLIIAHHPLLLKGVTAVSQLSRKGRIISDLIKSDLALFTAHT
                     NADVANPGVSDALATALGIEVDGPLEIETNLGRVGHLIKRIPLQDFAAQVESGLPKTA
                     RGIHIAGDASRSVENVAICGGAGDSLLDLVGQSNADVYVTSDLKYHVAEEFVQSTGKA
                     LIDISHWAGEWPWLNQAAQLLAQDLGSTLETQVSALATDPWTATIGHSANS"
     gene            3065..>3758
                     /locus_tag="AC16Y1_03330"
     CDS             3065..>3758
                     /locus_tag="AC16Y1_03330"
                     /inference="COORDINATES: protein motif:HMM:NF049746.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR5831526.1"
                     /translation="MPVIAGAEHQLKLLELAKVDTKLIQLDHQRVAIPEIKSVQDLEI
                     ELGSIEMRIVAAQTEVSDLAQAQNKAEIDVEQVQSRIDRDQKKLDDGTGSAKDLENLQ
                     HELGTLALRLKELEDVELEVMQQLEDAQNALAELDTEKNIVSVKLADIRKSLESKLNE
                     IELTISEIQTERTKLIQVLPTDLAELYEKIRSERGDVGAAEMIHGACQGCHLAIDATE
                     LNRIKDLPPDSVV"
BASE COUNT         1075 a          782 c          893 g         1008 t
ORIGIN      
        1 attagattgg atcccaatta cgggagtcga aaattaatcg atatctggtc agccaaattt
       61 gagattcttt ggggttaaaa atccaataac ctagacctgt ggaagcacag gggggtagcc
      121 cgagcgggtt agccggcaag ttcggatttg ccgaaggatt tgttgttcag gaaatcggct
      181 tcgatgtcga ttgcgataaa agcgtttcaa ctgcagcacg ttcagttgtt gagttagttg
      241 acgaagctta cgaagatatt gtcgatgcgg taatactttg gtggcgcgag gatgatggcg
      301 atctagttga tgcgctagtt gatgcgctca caccgcttgc agataatggc gttatttggc
      361 taatgactcc aaaagccggt cgcccagggc acgttgaacc aagcgatata gccgaggcag
      421 caccgaccgc aggtttacaa gcgactagca atataaatgc agcccctgat tggcagggaa
      481 ctagattagt agcaccaaaa gctcgtcgat aaattttcaa aaaactaaca cgttaaacaa
      541 ggtaggaaaa atgtcattag aagtcggcaa agaagcacca gactttacac tcaaaaacca
      601 atttggtgag gaagtaaaac tttctgactt cagggacaag aaaaatgtag tgttggtctt
      661 cttcccaatg gctttcaccg gaatctgcac aggtgagctg tgcgaaattc gtgacaatag
      721 tccagtattt gtttctgact cagtgcaagt cttgggtatt tcttgcgata cctctgctac
      781 tcacaaagtc tttgctgaaa aagaaaattt ggactatccg ttacttagcg acttttggcc
      841 gcatggtgcg gtttcgaaaa tgtacggttc attttttgaa gagcgcggtt tttcaacccg
      901 aggaaccttc attatcgaca agcagggtat tttgcgttgg tcagtagtta attcacctgg
      961 cgatgctcgc agtactgatg agtacaagaa agctttggct gaactttcct agccagtact
     1021 tgaaacgcat tgttttaagg taacaatcca atttattggg cgttttccct gtaaatcgct
     1081 ttcgaatcat ttggcaatgg tgtaaaacag atgtcagccg tgactggagt tgacccgaga
     1141 ctctccagtc acggtctttt tatgggcaca gtgtttattg gtgcccacgc aaatttatgg
     1201 tcgcactgtt taatggcgcc aaagcaaaaa ggacggtcga agaccgtcct tttttagcgt
     1261 gggcgctaag ggacttgaac ccctgaccct ctcggtgtaa acgagatgct ctagccaact
     1321 gagctaagcg cccgcgaagc cctaacttac ctaattaata gtcgttttgg aaatcgaaat
     1381 taactagttg acttttcaga taagttatgt tttaatagtt gaaaattcaa ctaaatgagg
     1441 aactatgtca gagttaatgc ccgcgctcta cattggacat ggcgcgccac cattactcga
     1501 cgatccggtt tggactaccg aactaaaaca actagcggca agtatgccaa cgccaaaagc
     1561 ggttttgatt gttagcgcgc attgggaatc tgctccgttg accatcgggg caactgaatt
     1621 tggcacacca ttgatatacg actttggtgg ctttgatcct aagtactaca acttgaagta
     1681 tgaaactcca gattcaactg ctttagctaa tttagtaaaa agtgttttgg ccgatactca
     1741 gacaattagc gagcaaccaa agcgcggtct tgatcatggc gcgtatgttc cgcttttgac
     1801 tatgtaccca gaggcaaaca ttccagtgct tcaaatgtcg attccaacac ataacccaat
     1861 tgagttagtc aagttaggcc aacgcttggc caaacttcgc gaacatggtg tgttgattat
     1921 tggtagtgga ttcttgactc atggcctgcc ttatttgcgc gattttagaa ttgaggccac
     1981 accaccaagt tggagtgttg agtttgaccg ttgggcacaa gaagccatcg gtaaaggcga
     2041 tctagacgta ttaatgaatt acaaaaatct tgctccgggt atgccttacg cacatccaac
     2101 agttgagcat ttgtcaccca ttttcgtaac attaggtgcg gctagcaatt caacagttgc
     2161 acccgaagtt gtcattgacg gctactggat gggcttggct aagacgtcaa ttcaggttgc
     2221 ttaatactcg ttaggcttta atcactatga gcctgaaact cacggacgtt atcgctgcaa
     2281 tggagcggcg gtacccaact gcaactgccc aagagtggga tgcagttgga ttagttgctg
     2341 gtaatccaga cagtgaaatc tcaaaagtat tgtttgccgt tgaccccagc gaagttgtgg
     2401 ttgagcaagc aattcaagta ggtgcgcagc taataatcgc gcatcatccg ttattgctca
     2461 aaggggttac agcagtttcg caattgagtc gaaaaggtcg gattattagc gacttaatta
     2521 aaagtgacct ggcgcttttt accgcacata ccaatgcgga cgttgctaac ccgggagttt
     2581 cagatgccct tgcgaccgca ctcgggattg aagttgatgg cccacttgaa attgaaacaa
     2641 atctaggccg ggttgggcat ctaattaaaa gaattccact tcaagatttt gcggcccaag
     2701 ttgaatctgg attaccaaaa acagctcgtg gcatacacat tgctggtgat gcaagtcgtt
     2761 ccgttgaaaa cgtggcaatt tgtggcggtg ctggagattc acttttggat ttggttggcc
     2821 aatctaatgc cgatgtttat gtaacttcag atttgaaata tcacgtggcc gaagaatttg
     2881 tgcaaagtac cggtaaggcg ctaatcgaca tctcacattg ggctggggag tggccttggc
     2941 tcaatcaagc cgctcaactt ttagcgcaag atttgggcag tactcttgag acacaagtta
     3001 gcgctttagc aacagacccg tggactgcaa ctatcggtca tagcgcaaat agttaggagt
     3061 aggaatgccg gtaatcgctg gtgctgagca ccaactcaaa ctgttggaac tagccaaagt
     3121 cgatacaaaa ttaattcaac ttgatcatca acgggtagca attcctgaaa tcaaatctgt
     3181 tcaggatctt gagattgagt tgggctcgat cgaaatgcga atcgttgcgg cacaaactga
     3241 agtatcagat ttagctcaag ctcaaaataa agctgaaata gatgttgaac aagttcaaag
     3301 tcgaatagat cgagaccaaa aaaaattaga tgatgggact ggctccgcca aagatttaga
     3361 aaatttgcag catgaattag gcactttagc attgcgcctt aaagaacttg aagatgttga
     3421 actcgaggtc atgcaacaac ttgaagatgc ccaaaacgca ctcgctgaat tagataccga
     3481 aaaaaatatt gtcagcgtaa aacttgctga catacgaaag tcactcgaat caaaacttaa
     3541 tgagattgag ttgacaattt ctgaaattca aactgaacga actaaactaa tccaggtact
     3601 tcctaccgat cttgctgaac tttacgaaaa aattcgctct gaacgaggtg atgttggcgc
     3661 ggctgaaatg attcacggtg cttgccaagg atgtcacttg gcaattgatg caacagaatt
     3721 aaatcgcata aaagacttgc cgccagattc tgttgtgc
//
LOCUS       DBNHVN010000172         1340 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_552342, whole genome shotgun sequence.
ACCESSION   DBNHVN010000172 DBNHVN010000000
VERSION     DBNHVN010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_552342"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="AC16Y1_03335"
     CDS             complement(<1..682)
                     /locus_tag="AC16Y1_03335"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018502144.1"
                     /note="catalyzes the phosphorylation and decarboxylation
                     of oxaloacetate to form phosphoenolpyruvate using GTP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HIR5831527.1"
                     /translation="MGTSAVPGLLPAPTENKKLISWVAEVAELTEPDRIEWCNGSEEE
                     WKRLTELLVDNGTFIRLNPEIRPNSFLARSNPNDVARVEDRTFICSIKEEDAGPTNNW
                     VEPSEMKETLRNIFKGCMRGRTMYVVPFSMGPLGSPISALGVELTDSPYVVVSMKMMT
                     RMGQKALDLIGPDGDFVPALHSVGAPLEPGQKDAAWPCSDTKYIVQFPEEREIWSYGS
                     GYGGNALLG"
     gene            878..>1340
                     /locus_tag="AC16Y1_03340"
     CDS             878..>1340
                     /locus_tag="AC16Y1_03340"
                     /inference="COORDINATES: protein motif:HMM:NF015423.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HIR5831528.1"
                     /translation="MQLEPQLVEPIALPLWIDLGAVALGAIYGATLATSRKAPLVGVL
                     LMGMLLGFGGSIIRDILLNAPINPLVKPNYVPTAAAFALVGAVFGNQILKPKRLFYTL
                     DALVIGMFVVIGAEKALIYNMPIGSVIFIGTLTAIGGGILGDILTGNKPDIM"
BASE COUNT          356 a          304 c          285 g          395 t
ORIGIN      
        1 tccctaataa tgcattgccg ccatatccag atccgtatga ccaaatttct cgctcttctg
       61 gaaactgaac tatgtacttt gtgtcactgc atggccaggc agcatctttt tgccccggtt
      121 caagcggtgc gccaactgaa tgaagtgcag gaacaaaatc accatctggt ccaatgaggt
      181 ctagtgcctt ttgacccatt cgcgtcatca tcttcatact cacaactaca tagggtgaat
      241 ctgttaattc aacacctaac gctgagatgg gtgaaccaag cgggcccatt gagaatggaa
      301 ctacgtacat agttcgacca cgcatacagc ccttgaaaat atttcgtaga gtctctttca
      361 tttcagatgg ttcgacccag ttatttgttg ggccagcatc ttcttctttg attgagcaaa
      421 taaatgttcg atcttcaact cgagctacgt catttggatt cgaacgtgca agaaaagaat
      481 tggggcggat ttctggatta agccgaatga aagttccatt atcaaccagt aactcagtta
      541 gtcttttcca ctcttcttct gagccgttgc accattcgat tcggtcaggt tcagttaact
      601 cggccacttc tgcaacccaa gaaattaatt ttttgttttc agttggtgct ggcaacaaac
      661 ctggtacagc cgatgtgccc atgaaaaccc ctagtctttc cgattagttt tattattggg
      721 gataagttaa agaatgcacc taaattcaaa aaaatcgaaa actggctttc cgaagtttcc
      781 ctttaaacat agacggtgtg accaagcaca catccaaata tggcattaac aagccggacc
      841 gataaccttt aattcaaatc agattctgga gtttcggttg cagttagagc ctcaattagt
      901 cgaacctatt gcgttaccgc tctggatcga tttaggcgct gtcgcacttg gcgcaatcta
      961 tggcgcaact ttggcaactt cacgcaaagc accactagtt ggtgtgctgc tgatggggat
     1021 gctcttagga ttcggtggca gcatcattcg cgacatattg ttgaatgcac ccattaatcc
     1081 tttggttaaa cctaactatg ttccgacggc tgcagcattc gcgttagtcg gagctgtatt
     1141 tggaaatcaa attttgaagc cgaagagact cttttacaca cttgatgcac tggttattgg
     1201 aatgtttgtt gtcattggtg ctgaaaaagc attgatctac aacatgccaa ttggatcagt
     1261 tatttttatc ggtacgctta cggcaattgg tggcggaatc cttggcgaca ttttgaccgg
     1321 gaacaaacca gacattatga
//
LOCUS       DBNHVN010000173         1635 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_624891, whole genome shotgun sequence.
ACCESSION   DBNHVN010000173 DBNHVN010000000
VERSION     DBNHVN010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_624891"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1164)
                     /gene="ychF"
                     /locus_tag="AC16Y1_03345"
     CDS             complement(91..1164)
                     /gene="ychF"
                     /locus_tag="AC16Y1_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008737296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR5831529.1"
                     /translation="MALTIGIVGLPNVGKSTLFNALTKNNVLAANYPFATIEPNTGVV
                     AVPDSRLEVLAKIFGSEKILPATVTFVDIAGIVRGASEGEGLGNKFLANIRESDAICQ
                     VIRAFNDPDVVHVDGKISPSSDIETINTELILADLQTLEKAITRLTKEAKQDKTKAES
                     LATAQKAAEILNSGKTLFASDLDVSPIRELFLLTAKPFIYVFNLDEDELVDESLKKEL
                     SQLVQPAEAVFLDAKLEADLTELPDEEALELLKSVGQQESGITVLSRVGFDTLGLQTY
                     LTAGPKESRAWTIKKGATAPEAAGVIHTDFQKGFIKAEVVSFEDLVTTGSMTEAKSQG
                     KVRIEGKDYVMKDGDVVEFRFNV"
     gene            1245..>1635
                     /locus_tag="AC16Y1_03350"
     CDS             1245..>1635
                     /locus_tag="AC16Y1_03350"
                     /inference="COORDINATES: protein motif:HMM:NF042451.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6542 domain-containing protein"
                     /protein_id="HIR5831530.1"
                     /translation="MSEITQTKNPGTHGWTYQGVFIILGVGLAVAMFADVFLNRKVDL
                     ISNVTLLVISVIAGWKVRTNDYLAAIFAPAFVWFVVLMTVGQLAPKRGGSLVREQVLH
                     IAYGLSAHAGWILSATLIAGGIAVVRRG"
BASE COUNT          427 a          341 c          343 g          524 t
ORIGIN      
        1 aatctgatca gttcggtgga gtttaattct taaatttcca ttccacggca gtattcattg
       61 ttgcgatgtt gtcaatgtat ttaggtttaa ctaaacatta aatctgaact caacgacgtc
      121 gccatccttc atcacataat ctttgccttc gatgcggact ttgccctgag acttggcttc
      181 tgtcattgat ccggtggtaa ctagatcttc aaaactaaca acttcagctt taataaaacc
      241 tttttgaaaa tcggtatgaa ttactccagc tgcttcagga gcggttgctc cttttttaat
      301 tgtccaggct ctagattctt ttggtcctgc agttaaatac gtttgcaagc caagtgtgtc
      361 aaagcccact ctcgataaaa cagttatgcc gctctcctgt tgtccaactg acttcaataa
      421 ttccaaggct tcttcgtcag gtaattcagt taaatcagct tcaagttttg catccaagaa
      481 aacggcctcg gcaggttgga ctaattggct caactctttt ttaagacttt catcaacgag
      541 ttcatcttca tctaaattaa aaacatagat aaaaggcttg gcagttagta aaaatagttc
      601 ccgaatcggg gacacatcta agtcggaagc aaataaagtt tttccgctat tcaatatctc
      661 ggctgctttt tgcgcagtcg ctaacgactc tgctttcgtt ttatcttgct tagcttcttt
      721 agttagtcga gtaattgctt tttctaaagt ttgtaggtct gccaaaatta attcggtgtt
      781 gattgtttca atgtctgaac tgggtgagat ttttccatca acgtgcacga catctgggtc
      841 attaaaggct cggataacct ggcagatcgc atctgactca cgaatgtttg ccaagaattt
      901 attgcctaaa ccttcacctt cacttgcacc gcgaacaatt ccggcaatgt ccacgaaagt
      961 aacggtggct ggcaaaattt tttccgaacc aaaaattttg gctaaaactt cgagtcgact
     1021 atctggaacg gcaacaactc ctgtatttgg ttcgattgtt gcaaagggat aattcgctgc
     1081 taagacatta tttttggtta acgcgttaaa taatgtcgac ttacctacgt taggtaatcc
     1141 cacaattcca atagttagtg ccacggctca taagcgtagt ggctatttaa acaaatgcgt
     1201 gattctggct caggtttaaa ttaaatagcg gtaacttatt acctatgtca gaaatcaccc
     1261 aaacaaaaaa tcctggaacc cacgggtgga cctatcaagg tgtgttcatt attctcgggg
     1321 ttggacttgc agtggccatg tttgctgatg tgtttttgaa cagaaaagtt gatttaattt
     1381 ccaacgttac gttgctagtg attagcgtga ttgctggctg gaaagttcgc actaacgatt
     1441 atttggctgc aatttttgcg ccggcttttg tgtggttcgt agttttaatg acagttggac
     1501 agttagcccc aaaaagaggg ggcagtttgg tgcgcgagca agtactacac attgcttatg
     1561 gtttatcggc acatgctggc tggatacttt cggccacact tattgccggc ggaatagccg
     1621 tggttaggcg cgggc
//
LOCUS       DBNHVN010000174         1780 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1444454, whole genome shotgun sequence.
ACCESSION   DBNHVN010000174 DBNHVN010000000
VERSION     DBNHVN010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1444454"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /gene="murA"
                     /locus_tag="AC16Y1_03355"
     CDS             complement(<1..1002)
                     /gene="murA"
                     /locus_tag="AC16Y1_03355"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR5831531.1"
                     /translation="MEVFRIQGGTRLAGQVRITGAKNSSLKLMAVALLATGKTTIRCV
                     PNILDVTIMAQLLRRLGCDVTYDANTEIVEISVPQNVEHRADYDLVRRMRASIAVLGP
                     LVARTGQADVALPGGDAIGSRGLDMHIAGLERMGAKVTNEHGYLVVHAPSTGLVGTQV
                     YLDFPSVGATETLLMAAVLAKGKTVIDNAAREPEIVDICEMLNKMGAQIEGVGTALLT
                     ITGVAELKPVVHDTVPDRIVAGTWAVAAAITQGDIEVVNANPEHISLALDKLVATGAQ
                     VSHTTDGFRVQMERRPKAVDIVTLPYPGFATDLQLQFIALNAIAEGQAMVTENLFEAR
                     "
     gene            1121..1696
                     /locus_tag="AC16Y1_03360"
     CDS             1121..1696
                     /locus_tag="AC16Y1_03360"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007384733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR5831532.1"
                     /translation="MVNLTRIYTRTGDDGTTNLGDMSRTGKNDPRLRAYADVDEANSA
                     IGLAIAAGKLRKDIVELLTRVQNELFDLGADLSTPVIDNPPHEPLRVTEQYVSRLEEA
                     CDKYNEELEPLRSFILPGGTVEASHLHVARTVVRRAERSTWAAIDMYHGGMNPLAATY
                     LNRLSDLLFILARVANKEIGDVLWQPGATRS"
BASE COUNT          477 a          437 c          389 g          477 t
ORIGIN      
        1 tctggcttca aataaatttt cagtaaccat cgcttggcct tcagctattg cattcagcgc
       61 aataaattgc agctgcaagt cagttgcaaa acctgggtaa ggaagcgtca cgatatcgac
      121 agctttaggg cgcctttcca tttgcactcg gaatccatcg gttgtgtgcg atacctgggc
      181 cccagttgct actaatttat cgagcgctaa agaaatatgt tccgggttag cgttaacaac
      241 ttcaatgtcg ccttgagtga tagctgctgc aactgcccaa gtgccagcaa caattcgatc
      301 tggcacggtg tcatgtacaa ctggctttag ttcagctacg ccagttattg tgagcaaagc
      361 agttcccacg ccctcaatct gcgcgcccat tttatttaac atctcgcaga tgtctacgat
      421 ttctggctca cgtgcagcat tatcaataac tgttttacct ttggccaaga cagctgccat
      481 taaaagtgtt tcagtagcac caacgcttgg aaaatctaag taaacctgtg taccaaccaa
      541 gcctgtgctc ggtgcatgca cgaccaagta cccatgttcg ttagtgactt tggctcccat
      601 tcgttcaagt ccggcaatgt gcatatctag tccgcgtgaa ccaattgcat cgccaccagg
      661 tagtgcgaca tcggcctgac ctgttctggc aactaacgga ccaaggactg caatcgaggc
      721 gcgcattctt ctaactaagt catagtcggc acgatgctct acgttctgcg gaactgaaat
      781 ctcaacaatt tcagtgttgg catcgtaagt aacatcgcaa cctaaacgtc ttaacaattg
      841 cgccatgatt gtgacatcta aaatgttggg gacgcaacga attgtggttt ttccagtagc
      901 aagcagagca accgccataa gttttagact gctgtttttt gctccagtaa tccgcacttg
      961 accagctagt ctggtgccac cttggatccg aaaaacttcc acgactaagc agattaccgc
     1021 ttattaacac tcaggtacat taggacagaa attaaatacc acttggtgct aataaccagt
     1081 tcattgcgca gtcctagcct catgccccta ggctttagac atggttaatc taactcgtat
     1141 ttacactcgc actggcgacg atggcaccac aaatctaggt gacatgagtc ggactggtaa
     1201 aaatgatcct cgcctacgcg catatgcaga tgttgatgaa gcaaatagtg caattggatt
     1261 agcaatagct gctggaaagt tgcgcaaaga tattgtcgaa ttactaactc gagttcaaaa
     1321 tgaactattc gacttgggtg ctgatttatc cactccggtc attgataatc ctccgcacga
     1381 accgcttcga gttactgaac aatatgtaag tcggcttgaa gaggcttgcg ataaatacaa
     1441 cgaagagcta gaacctttac gttcatttat tttgccaggc ggaacagtcg aagcctcaca
     1501 tcttcatgta gcaagaacgg ttgtgcgccg agcagaacgt agtacttggg cggcaataga
     1561 tatgtaccac ggtggcatga atcctcttgc tgctacttac ctcaatagac tttcagattt
     1621 actctttatt ttggctcgag tagcgaataa agaaattggc gacgtacttt ggcagccagg
     1681 agcaaccaga agttagtttc agccgattgc tttcgctaac cgctgcatcg ctgacgttag
     1741 tcggtcgtga aataaatcac cgtctagtcc ccacgcaacc
//
LOCUS       DBNHVN010000175         1502 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1667502, whole genome shotgun sequence.
ACCESSION   DBNHVN010000175 DBNHVN010000000
VERSION     DBNHVN010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1667502"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="AC16Y1_03365"
     CDS             <1..626
                     /locus_tag="AC16Y1_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HIR5831533.1"
                     /translation="GAGTQWLAEQKIGAEIDVVAPLGQPFALPKQPVGCVLVAGGYGA
                     APMMMLAQELKARGCKVEVVLGAATESKLFGVLDSKTIADTVTVTTDDGTSGVRGRVT
                     DVLPDAISRINAKAVYACGPMPMLQAVAGLSKELGAYSQCAVEEAMACGIGVCMTCVL
                     PVIGDDGVTRMVRSCVDGPVFRGDRVRWTDVDTVPVDAMGAPKVGGH"
     gene            626..>1502
                     /locus_tag="AC16Y1_03370"
     CDS             626..>1502
                     /locus_tag="AC16Y1_03370"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HIR5831534.1"
                     /translation="MSVDLTTALGSLQLVGPTATASGCAAAGKELSQFGDLTKLGAVV
                     TKSIMRDARSGRPTPRMAETPSGMLNSIGLQGPGIESFIEKDLTWLRENEVTTIVSIA
                     GNNVEEFGYLASRLVNEPGIAALEVNISCPNVESRGQVFACNPISAAAVIETVRSVWN
                     SPAPILAKLSPDVTDIVEIASSVVDAGAGGVSMINTLLGMVIDVDQMKPMLGGKTGGL
                     SGPAIRPVAVRAIYQVHAAFPQLPILGMGGVRTGHDALEFIAAGASAVSVGTVVFGDP
                     SAPWRVADELRTELEV"
BASE COUNT          372 a          310 c          419 g          401 t
ORIGIN      
        1 atggtgccgg aactcaatgg ttagctgaac aaaaaatagg tgccgagatt gatgtcgttg
       61 cgccacttgg ccaaccattt gccttaccca aacaaccagt tggttgcgtt ctagttgccg
      121 gtggttatgg cgcagctccg atgatgatgt tggcgcaaga acttaaagca cgcggttgca
      181 aagtcgaggt agtccttggc gcggcaactg aaagtaaatt attcggagtt ctggattcga
      241 aaactattgc cgacaccgta actgttacta cagatgacgg aacatctggt gtgcgcggtc
      301 gagtaactga cgttttaccc gatgcaatta gtcgaattaa tgccaaagcg gtttacgcct
      361 gcggtccaat gccaatgttg caagcagtag cgggcctttc aaaagaactt ggtgcgtata
      421 gtcaatgcgc agtcgaggaa gcaatggctt gtggcatcgg tgtttgcatg acttgtgtac
      481 tgccagttat tggtgatgac ggggttactc ggatggtgcg ctcatgtgta gacggaccag
      541 tattccgcgg tgatcgagtg cgttggactg atgttgatac tgttccagtt gatgcaatgg
      601 gcgctcctaa agtaggtggc cattaatgag tgtcgattta actaccgcac ttggcagttt
      661 gcagttagta ggtccaactg caacggcctc aggttgcgcg gctgcgggta aagaactttc
      721 gcaatttggt gacttaacca aacttggtgc ggtggttaca aaaagtatta tgcgagatgc
      781 cagatctggc cgaccaactc cacggatggc ggaaactcca agtggcatgc tcaactcaat
      841 tggattacaa ggcccgggta ttgaaagttt tattgaaaaa gatttaacct ggctgcgcga
      901 aaatgaagtc accacaatag tttcaatagc tggaaataac gttgaagaat ttggttactt
      961 agccagtcgg ttagtgaatg aaccagggat tgctgcgctg gaagttaaca tttcgtgccc
     1021 aaatgttgaa agtcgcggcc aagtttttgc ctgcaatcca atcagcgctg ctgcggtaat
     1081 tgaaacagtt cgcagtgttt ggaatagtcc agcaccaatt ttggccaagc tttcaccaga
     1141 tgtaaccgac attgtagaaa tcgctagcag cgtggttgat gctggtgctg gtggggtttc
     1201 aatgattaac acgttgcttg gcatggtgat tgatgttgat caaatgaaac caatgctagg
     1261 cggtaaaaca ggtgggctat ctggtccagc catcaggcca gttgcagtcc gcgcgattta
     1321 ccaagtgcac gctgcgtttc cacagttacc gattttaggt atgggcggcg ttcgaactgg
     1381 tcacgatgca ctcgaattta ttgcggctgg tgcaagtgca gtaagtgttg ggacagttgt
     1441 ttttggtgac ccaagtgcac cttggcgtgt tgctgatgaa cttcgaactg aacttgaagt
     1501 gc
//
LOCUS       DBNHVN010000176         2450 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_625326, whole genome shotgun sequence.
ACCESSION   DBNHVN010000176 DBNHVN010000000
VERSION     DBNHVN010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_625326"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="AC16Y1_03375"
     CDS             complement(<1..287)
                     /locus_tag="AC16Y1_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831535.1"
                     /translation="MAKENKSAKAAKQAAAAARRAAESDLKARDRKIRLIGGGVVVLI
                     MAGLLAIPFLNRGSEGSATDANAALPKGVTSDTYGVKIGTAWNSPDANSIPL"
     gene            complement(379..1158)
                     /gene="trpA"
                     /locus_tag="AC16Y1_03380"
     CDS             complement(379..1158)
                     /gene="trpA"
                     /locus_tag="AC16Y1_03380"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR5831536.1"
                     /translation="MSKLHDVFANAKANNRAALIAYLPAGYPTKAGAVECLTAMIRGG
                     VDIVEIGLPYSDPVMDGPTIQEAVDTSLKNGTKITDVIDTAKAVSDLGVPALVMSYWN
                     PIEANGVTNFTKALADAGGVGVITPDLTPEEAGDWIAATNEKLDRVFLVALSSTNERI
                     AKVCSATTGFIYAGAVMGVTGARETVLDSVKELVAKTRVHTDLPIAVGLGVSTPAQAK
                     EIAQYADGVIVGSAFVTAIKQGGAAAAEKLARELSAALTRD"
     gene            complement(1158..2366)
                     /gene="trpB"
                     /locus_tag="AC16Y1_03385"
     CDS             complement(1158..2366)
                     /gene="trpB"
                     /locus_tag="AC16Y1_03385"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR5831537.1"
                     /translation="MTTEAVLPDLTGHFGKFGGRFVPEALMAALDEITDAFGKAKVDP
                     VFQAQLHDLQVNYTGRPSPLTVASRFSEHAGGATILLKREDLNHTGSHKINNVLGQAL
                     LTKRMGKTRVIAETGAGQHGVATATAAALLGLECVVYMGEEDTKRQSLNVARMKLLGA
                     TVVPVTTGSKTLKDAINEAMRDWVANVHNTHYLFGTVAGPAPFPELVREFHRIIGDEA
                     RAQVLDRFGRLPDAVAACVGGGSNAIGIFTAFIDDKDVRLVGMEAAGDGVETGRHASS
                     ITGGTVGVLHGARTFVLQDADGQTVESHSISAGLDYPGVGPEHAWLADTGRAQYVPIT
                     DTQAMNAFKLLCETEGIIPAIETAHALAGVIDLGKELGPSGLILVNLSGRGDKDVQTA
                     AKWFGLEADS"
BASE COUNT          625 a          621 c          513 g          691 t
ORIGIN      
        1 agcggaattg aattggcatc tggcgaattc caggccgtac caattttcac gccataagta
       61 tcgctggtta cgcctttagg cagcgcagca tttgcatctg tagccgaacc ttcggagccg
      121 cgatttagaa atgggattgc aagtaagcca gccatgatta acacgaccac tccaccgccg
      181 attaatctaa ttttgcgatc gcgagctttt aagtctgatt cagcagctct gcgggctgca
      241 gcagcagctt gttttgcggc tttagcgctc ttattttctt ttgccattag attgcacctt
      301 cgttacttac ctgatacttc gcgttcttct agtttaattg cgaaccaatt aagcgcgcgt
      361 ttgcgcctat ttatcggatt aatcccgcgt taatgctgca cttaattcac gcgctaactt
      421 ttcagccgca gctgcgccac cttgtttgat ggcggtcaca aatgcactgc caacaattac
      481 cccgtcagcg tactgggcta tttcttttgc ctgtgctgga gttgatacac ctaggccaac
      541 tgcaatcggt aaatcggtgt gcacacgggt cttggcaact aattctttaa ctgaatctaa
      601 aactgtttct cgtgctccgg tgacacccat gacagcgccg gcataaataa acccggtagt
      661 tgccgaacaa actttggcaa tgcgctcatt agtactcgaa agtgcaacta agaaaactcg
      721 atccaacttt tcattagttg ccgcaatcca atcaccagct tcttctggag ttaaatcggg
      781 tgtaattact ccgactcccc cagcatcagc gagcgcctta gtgaaatttg tcacgccatt
      841 tgcttcaatt ggattccagt aactcataac taatgccgga acacctagat ctgataccgc
      901 tttagccgta tcaataacgt ccgttatttt tgttccattt tttagcgaag tatcaacagc
      961 ttcttgaatt gtggggccgt ccataaccgg gtcgctatac ggtaaaccga tttcaacaat
     1021 atctacgccg ccgcgaatca ttgcggttaa gcattcaacg gctccggctt ttgttggata
     1081 gccagccgga agataagcaa ttaatgccgc gcgattattt gcttttgcat tagcaaatac
     1141 gtcatgcagt ttgctcatta actgtcagcc tcaaggccaa accatttcgc cgcagtctgc
     1201 acatctttgt cgccacgacc agaaaggtta accaaaatta atccgcttgg acctagttct
     1261 ttaccaagat cgattacccc agcaagtgcg tgtgcggttt caatcgctgg aataattcct
     1321 tcagtttcgc aaagtaactt gaatgcattc attgcttgag tgtcagtaat cggaacatat
     1381 tgtgcccggc cagtatctgc taaccaagca tgctctggtc caacacctgg ataatctaag
     1441 ccagcactaa ttgagtgact ttcaactgtt tgtccatctg catcttgtag tacaaatgtg
     1501 cgtgcaccat gtaacacgcc tacagttcca ccagttatcg aagaggcatg acgaccagtt
     1561 tcaacaccat caccggcagc ttccatgcca actaagcgaa catctttgtc gtcaatgaaa
     1621 gcggtgaaaa taccaattgc atttgaaccg ccaccaacac atgcggcaac tgcatcagga
     1681 agtcgaccaa agcggtcaag tacttgagca cgcgcttcat caccaattat tcggtggaat
     1741 tctcgaacta attctgggaa tggcgctgga ccagcgacgg tgccaaataa gtagtgcgtg
     1801 ttatgcacat tggcaaccca gtcacgcatg gcttcattaa tcgcatcttt taatgtttta
     1861 cttccggtgg ttactggaac aacagttgcg ccaagtaatt tcatccgagc aacgtttaag
     1921 gattggcgtt tagtatcttc ttcgcccatg tagacaacgc attccaagcc aagaagtgcc
     1981 gctgccgttg cagttgctac gccatgttga ccagcaccag tttcggcaat aactctggtt
     2041 ttacccatgc gctttgttag taatgcttgg ccaagtacat tgttaatttt gtgcgaacca
     2101 gtgtgattta aatcttcacg ctttaacaat atagttgcgc caccggcatg ttcagaaaat
     2161 ctgctggcta ccgtaagtgg gcttggtcga ccggtgtaat taacttgcaa gtcatgaagt
     2221 tgcgcttgaa agactggatc tactttggct ttaccaaatg catcagtaat ttcatcgagc
     2281 gcagccatta aagcttcagg aacaaaacgg ccaccaaatt ttccaaagtg cccagttaag
     2341 tcaggcaaaa ctgcttctgt agtcatacct aactcctcat catttcggca gctaactgca
     2401 taccgacttc agtccattct tgaatagttt cggtgggatt gccactttta
//
LOCUS       DBNHVN010000177         2663 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1073667, whole genome shotgun sequence.
ACCESSION   DBNHVN010000177 DBNHVN010000000
VERSION     DBNHVN010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1073667"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /gene="tyrS"
                     /locus_tag="AC16Y1_03390"
     CDS             <1..924
                     /gene="tyrS"
                     /locus_tag="AC16Y1_03390"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019067409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR5831538.1"
                     /translation="NAAIMSNNYIWTHNMDVLTFLRDIGKHFSVNRMLDKESVSARLN
                     GAGISYTEFSYQILQSLDFLELYRRHNCTLQIGGSDQWGNITAGTDLVRRIESVSVNG
                     LTTPLVTKADGTKFGKTESGTVWLDPEMTSPYAFYQFWINADDRDADQLLRTFTFRSK
                     DEIEQLAVATQERAHAREAQRTLARDVTTLVHGQHATNQAEAAAGALFGQGELRELDA
                     STLDAAMSEAPNATIRAQGELPTVAELLVACELVQSNSAGRRAINEGGVSVNNVKITD
                     EDHRVSKDDFLHGKWLVLRRGKRTFGGIRLS"
     gene            1006..1710
                     /locus_tag="AC16Y1_03395"
     CDS             1006..1710
                     /locus_tag="AC16Y1_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831539.1"
                     /translation="MSPAKKPVKKKVVKKTTKKAVKKSVKKAVKRPAKKTAKKAVKRK
                     PAKKAANKPLAKKATQPAAPKSADTKSAAANFDESLEKLNAWAKKTFNTLFNFTPEDN
                     SKQKLAAVDPTFRRIIDNSNPEIVSWVSVGKIKEDGKSGSATRKWLLLMSNGDLSYVN
                     RGTTRSPENAISASGKNVKVIVNANAFVKSVSFDGYKMLLNGVSNKVIEVSLDPKGKP
                     SLEAAKKFATKVKKFI"
     gene            complement(1707..1946)
                     /locus_tag="AC16Y1_03400"
     CDS             complement(1707..1946)
                     /locus_tag="AC16Y1_03400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000987 - cis-regulatory region
                     sequence-specific DNA binding [Evidence IEA];
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0044010 - single-species biofilm formation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HIR5831540.1"
                     /translation="MSKDTTINIRIDAKTKKKAEKIFARCGYTTSTAIRLFLLRTIKE
                     NDWPFDIQVTSDELATSQNKDETKVKNNSKQDAKS"
     gene            2406..>2663
                     /locus_tag="AC16Y1_03405"
     rRNA            2406..>2663
                     /locus_tag="AC16Y1_03405"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          793 a          580 c          609 g          681 t
ORIGIN      
        1 aatgctgcga tcatgtcgaa taactacatc tggacccaca acatggatgt cctaactttc
       61 cttcgcgaca ttggcaagca cttcagcgtc aatcgcatgc ttgataaaga atcagttagc
      121 gctcgactca atggtgcagg tatttcgtat accgaattta gttaccaaat tttgcagtcg
      181 cttgatttcc tcgagttata ccgtcggcac aattgcacat tgcaaattgg tggcagcgat
      241 caatggggca acatcactgc gggtactgat ttagttcgtc ggatcgagag cgtttcggtt
      301 aatggtctaa ctactccact cgttaccaaa gctgatggca caaagtttgg taaaactgaa
      361 tctggcacag tgtggcttga tccagagatg acttcgcctt atgcgttcta ccaattttgg
      421 attaacgcag atgatcgaga tgccgaccaa ttacttcgca cttttacttt ccgctctaaa
      481 gatgaaatcg agcagttggc cgttgccacc caagagcgcg cacacgcccg cgaagcccag
      541 cgcacacttg ctcgtgatgt gaccacattg gtgcacggtc agcatgcaac aaaccaagct
      601 gaagccgccg ccggtgcact attcggtcaa ggtgaattgc gtgaactgga tgcctcaacc
      661 cttgatgctg cgatgagcga ggcacctaat gccacgatca gggcccaagg ggagttaccg
      721 acagtggctg aattgcttgt ggcctgcgaa cttgtgcaga gtaactctgc gggccgccgg
      781 gcgataaacg aaggtggtgt ctcggttaat aatgtaaaaa tcaccgatga agatcaccga
      841 gttagtaaag atgatttctt gcatggcaaa tggttagtcc ttcgccgcgg caaacgcacc
      901 tttggtggaa ttcgcctttc ctaatgggat aaagccttta ttaagtggca aaaagttggg
      961 atttccctcg tactactgaa gccaaaatat cgctaaactc aacccatgag tccagcaaag
     1021 aagcctgtga agaaaaaggt cgtcaaaaag acaaccaaaa aagcagttaa gaagtctgtg
     1081 aaaaaggcgg taaagcggcc tgccaaaaag acagccaaaa aggctgtaaa gcgcaagccg
     1141 gccaagaagg ccgcaaacaa gcccttggct aaaaaggcaa ctcaaccagc cgcgcctaag
     1201 tctgcagata ctaagtcagc cgcagcgaac tttgacgagt cattagaaaa attaaatgcc
     1261 tgggcgaaaa agacttttaa cacccttttt aatttcaccc cagaagataa ttcaaagcag
     1321 aaactagctg ctgttgatcc aactttccgt cgcattatcg acaactcaaa cccagaaatt
     1381 gtttcttggg tttcagtagg caagatcaag gaagacggca agtcaggatc tgcaacgcgc
     1441 aagtggcttt tactaatgtc caatggcgat ttgagttacg tcaaccgcgg tacaactcgt
     1501 tcaccggaaa atgcaattag cgcatctggc aagaatgtaa aagtaattgt taatgcaaat
     1561 gcatttgtta aatcggtttc gtttgatggt tacaaaatgc tacttaacgg agttagcaac
     1621 aaggtcatcg aagtttcgct tgatccaaaa ggtaaaccaa gtcttgaagc ggcaaagaag
     1681 ttcgctacaa aagtaaagaa gtttatttaa ctcttcgcat cttgctttga gttatttttt
     1741 acttttgttt catctttatt ttgtgaagtt gcaagttcat cacttgttac ctgaatatca
     1801 aacggccaat cattttcttt aatagtgcga agtaaaaata atctaatcgc agtcgaagtt
     1861 gtataaccac aacgagcaaa aatcttttca gctttctttt ttgtttttgc atcaatccga
     1921 atattgattg tggtgtcctt gctcatgact tcctcgaaat taatttgtgc gcacaatgta
     1981 tatacaaatt attccagtta gcgattcgcc aagatagcct gcaaaagggg aataaatcag
     2041 ccctaaaaag tggttgtgga aagtggtaaa aaacccttaa atttgcgctt tttttggaaa
     2101 agacgtagat tatgggaacg atcactacct agcgaaaaag ccccaaaaag ctttgaaaat
     2161 gctgctgatt cgggatttga ccgaatctaa caaagagcgt aagttaggga gtcctgcctc
     2221 gaaaaaagcc tagaaatagg cggatttcat gcgcactcgt accttgagaa ctcaacagcg
     2281 tgccacaagt caataccaaa ccccgtttgg taggcgtaag cctgcctaat ggaaattcct
     2341 taattggtaa aagacaaaaa caaataagca gttcagcttg tttgaactat tgccagaatc
     2401 actctttaac ggagagtttg atcctggctc aggacgaacg ctggcggcgt gcttaacaca
     2461 tgcaagtcga acgatgaaag agagcttgct ctctggatta gtggcgaacg ggtgagtaac
     2521 acgtgactaa cctgcccctt tctctgggat aacgcttcga aaggagtgct aataccggat
     2581 acgaactctg aacacatgtt cggagttgga aagtttttcg gaaagggatg gagtcgcggc
     2641 ctatcagctt gttggtgagg taa
//
LOCUS       DBNHVN010000178         7839 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1518999, whole genome shotgun sequence.
ACCESSION   DBNHVN010000178 DBNHVN010000000
VERSION     DBNHVN010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 7839)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7839)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7839
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1518999"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="AC16Y1_03410"
     CDS             complement(<1..732)
                     /locus_tag="AC16Y1_03410"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010267447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR5831541.1"
                     /translation="MRLYDTAARSVREFTPLVPGQVSMYVCGATVQAAPHVGHIRSGV
                     AFDVLGRWFRATGYQVTLVRNVTDIDDKILAKGVTENRPWWAVAAHYEREFQNAYAIL
                     GCTPPTVEPRATGHITQMIDLMEQLIVAGHAYAVDGDVYFDVRSFADYGKLSGQKIDD
                     LLPAGDSEGEARKKDSRDFALWKSAKPGEPAWPTPWGSGRPGWHIECSAMAHAYLGNS
                     FDIHGGGLDLVFPHHENEVAQSKAAG"
     gene            complement(752..1231)
                     /gene="ispF"
                     /locus_tag="AC16Y1_03415"
     CDS             complement(752..1231)
                     /gene="ispF"
                     /locus_tag="AC16Y1_03415"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR5831542.1"
                     /translation="MNNLRIGTGVDVHPLVAGRPMYVACLEWPGETKGPEGHSDGDVA
                     AHAVCDALLSAAGLGDLGSVFGTNDPKWSGASGVTLLAEVHRKITEAGFDIVNVSVQV
                     IGNSPKIGPRRLDAQKAMSSALYGAQVSVSGTTTDGLGLTGRGEGIAAIASVLLSIR"
     gene            complement(1224..1913)
                     /gene="ispD"
                     /locus_tag="AC16Y1_03420"
     CDS             complement(1224..1913)
                     /gene="ispD"
                     /locus_tag="AC16Y1_03420"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HIR5831543.1"
                     /translation="MNNAATGRVAAIIPAAGSGERFGAQIPKAFIELDGISLLTRSAL
                     AMSVVADVIVVAAPSNQLELASEHLVNVDAEVHVVAGGSARQDSIANALNVLPDDVEF
                     VLVHDAARPLVPIEVTKRVLAEIQNGAIAVIPVLPIADTIKRVDAQNNVVETVDRNQL
                     RRVQTPQGFTRQTLHQAYADPTIIATDDAGLMEALGITVLTVAGDELAMKITTSQDLQ
                     IASALLGVTNE"
     gene            complement(1913..2395)
                     /locus_tag="AC16Y1_03425"
     CDS             complement(1913..2395)
                     /locus_tag="AC16Y1_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009303 - rRNA transcription [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR5831544.1"
                     /translation="MAYKVGETVVYPHHGAADIISKETRVVKGEKKEYLVLKVHQSDL
                     TVRVPSDNLELVGVRDVTSQEGLEKVFGVLRQPYVEDPANWSRRFKANTEKLSSGDVI
                     KVAEVVRDLWRRSQKKPLSTGEKSMLARARTQLISEIALAEHTNEVRAEEILDEVLAS
                     "
     gene            2543..3055
                     /locus_tag="AC16Y1_03430"
     CDS             2543..3055
                     /locus_tag="AC16Y1_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831545.1"
                     /translation="MKKVFLTFALVSSLALSGCSTGFNAATNTQGNSGNGRTANVDTI
                     QIRNAVIIVDAQDPTRATLIATVRNIGEETEILKAVELDPAITVSMEEIELVKGNAVS
                     IGYNSDVKIALSSIGESLIAGQWTAVKFVFGNNAPIEMSLLVKPNKDYYSDVEIPTVE
                     VAPSPTPSAS"
     gene            complement(3064..3474)
                     /locus_tag="AC16Y1_03435"
     CDS             complement(3064..3474)
                     /locus_tag="AC16Y1_03435"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HIR5831546.1"
                     /translation="MKKVTVLFVCVHNAGRSQMAAGYLNHLAGDRIQVLSAGTEPEDQ
                     INPVAIEAMAEEGIDIANNSPKVLTEEAVKDSDYVITMGCGDKCPFFPGKTYLDWPLK
                     DPAGQSIEDVRPIRDEIRTKVENLIKEIDSKIIS"
     gene            complement(3476..4165)
                     /locus_tag="AC16Y1_03440"
     CDS             complement(3476..4165)
                     /locus_tag="AC16Y1_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006358524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5831547.1"
                     /translation="MIKILVIEDEESFREALEFILTKEGFQIECAADGLAGLAAFEKY
                     NPDIILLDLMLPGMSGTDVCKTIRAKSNVPIIMLTAKDAEIDKVVGLEVGADDYVTKP
                     FSSPELVARIRAVLRRGTIDVQDAGDVIELGPIKLDSARHSVQVSGQEVTMPLKEFEV
                     LEYLMDNAGRVLTRGQLMDRIWGSNYVGDGKTLDVHIKRIRSKIEKDPANPTLLHTVR
                     GLGYKFEVSNS"
     gene            complement(4165..5301)
                     /locus_tag="AC16Y1_03445"
     CDS             complement(4165..5301)
                     /locus_tag="AC16Y1_03445"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5831548.1"
                     /translation="MPIVEVLLAAVTLGLVVTLFRFRAFKKQIKLQNLDVELIRNRAL
                     KDIVELIPSAAMIADEKNQVITYNSACIPLRLISDDRISIPEIRELNEQVGKTKNAQT
                     IEQLFITTDPTIPDWEAAVAISPIDDKYSLVLITDLSEERRLNEVRRDFVANVSHELK
                     TPVGALSLLAEAITAAGTDQEQIKKFSTRMQLEVKRLTELITDLVELSEVQGDKLMRN
                     FEPVNLVSVINESVDVAKLFAQEKKININSPQNLAAVTVLGDQRQLVTALSNLITNAI
                     RYSPAHTNVGIGLRTENDQAMISVTDQGVGISEADQVRIFERFYRVDPARSRETGGTG
                     LGLAIVKYICANHGGECTVWSRKNQGSTFTLNLPIYSGEIQRGN"
     gene            5457..6104
                     /gene="phoU"
                     /locus_tag="AC16Y1_03450"
     CDS             5457..6104
                     /gene="phoU"
                     /locus_tag="AC16Y1_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR5831549.1"
                     /translation="MREDYQLQLESINIDLVAMTELVGKAMSKATSSLLNDDLQLAEQ
                     VIAGDELINSLNSSIEERSLQMVALQAPVARDLRILMSAIRMGSSLERMGDLASHISK
                     QVRLRYPNPSIPAELHETFARMGEAATKIVAETGRVIKTRDADLAEKIKVYDDELDNL
                     HRELFSVVLSDDWAHSVEAAIDVTLLSRYYERFGDHAVTVARRVVHIVTGEPYTK"
     gene            6177..7442
                     /gene="mshA"
                     /locus_tag="AC16Y1_03455"
     CDS             6177..7442
                     /gene="mshA"
                     /locus_tag="AC16Y1_03455"
                     /EC_number="2.4.1.250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015796772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol-3-phosphate glycosyltransferase"
                     /protein_id="HIR5831550.1"
                     /translation="MTKRLERIAILSVHTSPLHQPGTGDSGGMNVYIAETSKRLAQRG
                     IEVEIFTRATSPKDEEHVVLAPGVTVKHIPAGPFEGLRKEDLPAQLCAVTAGVLRAEA
                     NKLEGFYDLLHSHYWLSGQVGWIAQERWGVPLVHTMHTMARVKNLNLADGDTPEPTIR
                     EIGEEQVVQAADRLVANTQTEAQELIELYGAQPERVRVVHPGVDLTNFVPGDKSAARH
                     ELGIAANAVVMLFVGRIQPLKAPDVLLKAAAELIRHQPELRNRLVVPICGGPSGTGLA
                     RPDALHKLAAELGISENVIFEPPTNRARLVTWLQAADVCLIPSYNESFGLVALEAQAC
                     GTPVIAANVGGLPTAVSNNYSGLLVDGHNPHEWAHVLEKYFADQKLQEKLRAGALDHA
                     AKFSWEATTERLIEVYEEAIDSAIARRNA"
     gene            7442..7813
                     /locus_tag="AC16Y1_03460"
     CDS             7442..7813
                     /locus_tag="AC16Y1_03460"
                     /inference="COORDINATES: protein motif:HMM:NF022182.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone family
                     protein"
                     /protein_id="HIR5831551.1"
                     /translation="MISLDQLTDYLASQDLAPQDISDTALVVTVQGTNKLKINVAISL
                     GQHTLRIESFVARNPDENHENVYRWMLEENRKLLTVAFCLDTFGDIYLRGSLPIENLT
                     LSDVDQILGVVIAQSDRSFNP"
BASE COUNT         2208 a         1738 c         1642 g         2251 t
ORIGIN      
        1 accagcagct tttgattgcg cgacttcatt ttcatgatgt gggaaaacta aatctagacc
       61 gccaccgtga atatcaaatg agttacctaa ataggcatga gccattgctg agcactcaat
      121 gtgccaacct ggccgacctg atccccatgg agttggccaa gcaggttcac ccggttttgc
      181 agatttccaa agtgcaaagt cgcgtgaatc ttttttacgg gcttcacctt cggaatcgcc
      241 ggctggaagt aagtcgtcta ttttctggcc ggataatttt ccgtaatcag caaatgagcg
      301 cacatcaaaa taaacatcgc catcaactgc ataagcatgg ccagcaacaa ttagttgctc
      361 catcaaatcg atcatttggg taatgtgacc agttgctcgc ggttctactg ttggcggtgt
      421 gcagcccaga atcgcataag cattttgaaa ttcgcgttcg taatgcgctg ccaccgccca
      481 ccagggacga ttttcggtca caccttttgc caaaatcttg tcgtcaatat ctgtgacatt
      541 tcgcaccaaa gttacttggt agccagtggc tcgaaaccaa cggccaagta catcaaaggc
      601 cacaccggaa cgaatgtgcc cgacgtgcgg agctgcttga actgttgcgc cgcagacata
      661 catcgaaacc tgaccgggca cgagaggagt aaattcccga accgatctag cagcagtgtc
      721 ataaaggcgc acggaataag ggtactaact attagcggat agacagtaat actgaagcga
      781 tggcagcgat accttcaccg cgaccagtta aaccaaggcc atcagttgta gtaccagaaa
      841 cagaaacttg cgcaccatat aaggcactac tcattgcttt ttgggcatca agacggcgtg
      901 gaccaatttt tggactgtta cctattactt gaactgaaac gttcacgata tcaaagccag
      961 cttcggtaat ctttcggtgc acttcagcca gcaaagttac gccacttgcg ccactccact
     1021 ttggatcgtt ggtaccgaaa actgaaccta aatcacctag gcctgcagct gatagcaacg
     1081 catcacaaac agcatgggct gctacatctc catcggagtg accttctgga cctttagttt
     1141 caccaggcca ctcaagacat gcaacataca ttgggcgacc agcaactaat ggatgaacat
     1201 caacaccagt tccaattcgt aaattattca tttgttactc caagtagcgc gctggcaatt
     1261 tgtaaatctt gactggtggt tattttcatc gctagttcat caccggcaac tgttaacact
     1321 gtgattccta gtgcttccat taacccagca tcatcagtgg caatgattgt tggatctgca
     1381 tacgcctgat gtaaagtctg gcgcgtaaaa ccttgcggag tttgcactcg acgaagttgg
     1441 ttgcgatcga ctgtttcgac aacattattt tgtgcatcaa cacgtttaat tgtgtcggca
     1501 atcggcagaa ctggaataac tgcaatggca ccgttttgaa tttcagctaa aactcgttta
     1561 gtaacttcta ttggcactaa tggccgagca gcatcatgaa ctaaaacaaa ttcaacatca
     1621 tctggcaaaa catttaatgc atttgcaatt gaatcctgcc tggcacttcc gccagcaact
     1681 acatgaactt cagcgtcaac attcactaaa tgctcgctag ccaactcaag ttgattactt
     1741 ggcgcggcaa ctacgatcac atctgcaaca acactcatag ctaatgcgct acgagttaat
     1801 aatgaaatgc catctagttc aataaatgct tttggtattt gtgcgccaaa gcgctcacca
     1861 gaacctgcgg caggaataat tgccgccact cggccagtag cggcgttatt cattaggaag
     1921 ctaatacctc gtcaagaatt tcttcggccc gaacttcgtt agtgtgttca gctaaagcaa
     1981 tttcgctgat tagctgggtg cgggcgcgtg ccaacatact cttttcacca gttgataatg
     2041 gcttcttttg gcttcggcgc cataaatcgc gaactacttc ggcaactttg atgacatcgc
     2101 ccgaagataa tttttcagtg ttggccttga aacgacgcga ccagtttgct ggatcttcga
     2161 cataaggctg acgtagaacg ccaaaaactt tttctagacc ttcttggctc gttacgtcac
     2221 gaacaccaac tagttctaag ttgtccgatg gcacccgaac agttaaatca gactgatgaa
     2281 ctttaagaac taagtattct ttcttttcac ctttaactac gcgagtctct ttcgagataa
     2341 tgtctgctgc accgtgatgc gggtatacga cagtttcgcc aactttatag gccatttatg
     2401 ggttcctttt tatttctgaa ctgcaattct accacgagca aagattgtct aatttcaaga
     2461 aaaccccgat ttaattggct tttaatcaac tactaggctt gagatcaaga ccattgaatt
     2521 tcaggagttg gaccacgtcg tcatgaaaaa agtttttctg accttcgcat tagttagttc
     2581 acttgcccta agcggttgct caactggatt taatgcagca actaatactc aaggtaactc
     2641 tggcaatggt cgcactgcca atgtcgacac tattcaaatt cgcaatgcag taattattgt
     2701 ggatgcgcaa gatccaacac gagcaacgtt aattgccact gtgcgcaata ttggcgaaga
     2761 aacggaaatc cttaaagctg ttgaacttga tccagcgatc acagtttcaa tggaagaaat
     2821 cgaattagtt aaaggtaacg cagtctcaat cggttacaac tctgatgtga aaattgcact
     2881 tagttcaata ggtgaatcac ttattgctgg acaatggact gcagtgaaat ttgtattcgg
     2941 taataatgca ccaattgaaa tgtcgttatt agtaaagcca aataaggatt attactcaga
     3001 cgttgagata cctacagttg aagtagcgcc tagcccaaca ccaagcgcta gctaaagact
     3061 taattaagaa attatttttg aatcaatttc tttaattaga ttttctacct tggtgcgaat
     3121 ttcatctcga attggcctga catcttcaat actttgacct gctggatctt ttagcggcca
     3181 atctaaatat gtcttgcctg ggaaaaatgg acatttatcg ccacagccca ttgtgattac
     3241 ataatctgaa tcttttacag cttcttcagt taaaactttt ggactgttgt ttgcaatatc
     3301 aattccctct tcggccatgg cttcaatggc aactgggttg atctgatctt caggttcagt
     3361 cccagctgag agtacttgga ttcgatcacc ggcaagatga tttaaataac ctgcggccat
     3421 ttgagaacga ccagcattgt ggacacaaac aaatagaacg gtaacttttt tcaagttagg
     3481 aattacttac ttcaaatttg taaccgaggc cgcgcacagt gtgcagcaaa gttggattcg
     3541 ctggatcttt ttcaattttt gaccgaatac gtttgatatg cacgtctaaa gttttgccgt
     3601 cgccaacata gtttgaaccc cagatacgat ccattaattg gcctcgagtt aatacacgcc
     3661 cagcgttatc cattaagtat tcaagtactt caaattcttt tagcggcatc gttacttctt
     3721 ggccgctgac ttgaacacta tgtcttgcgc tatctaattt gattggccca agttcaatca
     3781 catcgccggc atcttgcaca tcaatagtgc cgcggcgaag tactgctctg atacgagcga
     3841 ctagttctgg cgatgaaaat ggcttagtta cataatcatc ggcgccaact tcaaggccaa
     3901 caactttgtc tatctcggca tctttagcag tcagcataat tattggcaca ttactttttg
     3961 cccgaattgt tttgcaaaca tctgtgccag acattccggg caacatcaga tctagcaaaa
     4021 taatgtctgg attgtatttt tcaaatgcag caagcccagc taagccatca gcagcgcatt
     4081 caatttgaaa gccttcttta gtaagaataa attcaagggc ttcgcgaaat gattcctcat
     4141 cttcaataac taaaattttt atcattagtt acccctttga atttcgcctg aataaattgg
     4201 caaattaagt gtgaaagttg acccttgatt ttttcgtgac caaactgtgc attcgccacc
     4261 atggtttgca caaatatatt taacaattgc taaacctaat ccggtaccac cagtttcacg
     4321 acttcgtgct ggatcaacac gatagaaacg ttcaaatatt ctgacctgat cagcttcgct
     4381 aatccctacg ccctggtcgg taactgaaat catggcttga tcattttcag tgcgcaatcc
     4441 gatacctacg tttgtatgtg ctggagaata gcgaattgcg tttgtaatta aatttgaaag
     4501 tgcggtgact aattggcgct gatcaccaag taccgtaacc gcagctaaat tctgcggtga
     4561 atttatattg atcttttttt cttgggcaaa aagtttggct acatctactg attcattaat
     4621 aactgatact agatttactg gctcaaagtt gcgcattaac ttatcgcctt gtacttcact
     4681 taattcaact aagtcagtaa tcaattcagt cagacgttta acctctaatt gcatccgggt
     4741 cgaaaacttt ttaatttgtt cttgatcagt gccagctgcg gtgattgctt cggcaagtaa
     4801 ggaaagtgct ccgacgggtg tttttaattc gtgtgaaacg ttagcaacaa aatcgcggcg
     4861 aacctcgttc aaccttcgtt cttcacttaa atcggtgatt aaaactaatg aatacttgtc
     4921 atcgattggt gagatagcaa cggctgcttc ccaatctggg attgttggat cagtagtaat
     4981 aaaaagttgc tcaattgttt gagcgttctt agttttacca acttgctcgt tgagttcgcg
     5041 gatttctggg atcgaaatac gatcatctga aattaagcgc aatgggatac acgctgagtt
     5101 atatgtgatg acttgattct tttcatctgc gatcatcgcc gcacttggga tcagttcaac
     5161 tatgtcttta agtgctcgat ttcgaattaa ttcaacatct aagttttgta atttaatttg
     5221 ttttttaaat gccctaaatc ggaacagggt tacgactaat cccagagtga ctgcagcaag
     5281 cagaacttcg acgatcggca cagtttaaga atagataatg gccaagggtc agatgaactc
     5341 gataggtgaa cttaactttt tgttcactta atgcttgcta agaattcact gaattttgcc
     5401 gattaaccgg cactaacgaa caaaaccatt taagtatctc gggtaaagta aaggctatgc
     5461 gcgaggacta ccaactacag cttgagtcaa tcaatatcga cttagttgct atgactgaac
     5521 ttgtcggtaa ggcaatgtct aaagcaactt cttcattact aaacgatgat ctacaacttg
     5581 ccgaacaagt tattgctggt gacgagttaa ttaactcgct gaactcttca attgaagaac
     5641 gcagtttgca gatggtggca cttcaggctc cggtagcgcg tgatttacgt attttaatga
     5701 gtgcaattcg catggggtct tcacttgaac gtatgggtga tttagcctca catatttcta
     5761 aacaagttcg cttgcgttac ccaaatccat ctatcccggc tgaactacat gaaacttttg
     5821 cccggatggg cgaagccgct acaaaaattg tggctgaaac tggtcgagtt attaaaaccc
     5881 gcgatgctga cttagctgaa aaaattaaag tttatgacga tgaattagat aacttacatc
     5941 gcgaactatt tagcgttgta ctttctgatg actgggcaca cagtgttgaa gccgcaatcg
     6001 acgtgacttt actatctcgc tattacgaac gatttggcga tcacgcagtt accgtagctc
     6061 gtcgcgtagt ccatattgtg actggcgagc cttatacaaa ataaaacctc aataaataac
     6121 tgaaggtttt tgctcaacta tctataacca caacacttcg ataaattaga agtgcaatga
     6181 caaaacgttt agaacgtatc gcaatcttgt cggtacatac ttccccgcta caccaacctg
     6241 gtactggcga ttctggtggc atgaatgttt atattgccga aacttctaaa cgcctagctc
     6301 agcgcggcat tgaagttgaa atatttactc gggcgacttc accaaaagat gaagagcatg
     6361 ttgtgctcgc gccaggtgtg actgttaaac atatcccagc cggaccattc gaaggtttac
     6421 gtaaagaaga tttacctgca caactttgtg cagtgactgc aggtgtgctg cgcgctgaag
     6481 caaataagct cgaaggtttt tacgacttgc tgcacagcca ttactggtta tccggccaag
     6541 ttggctggat cgctcaagaa cgctggggcg ttccactagt tcacactatg catacgatgg
     6601 ctcgggttaa gaatttgaat ttagctgacg gtgacacacc cgaaccaact attcgagaaa
     6661 ttggtgaaga acaagttgtt caggccgctg atcgactagt agctaatact caaactgaag
     6721 cacaagaatt aatcgaactt tatggcgcgc agcctgaacg ggtacgcgtt gtgcatcctg
     6781 gagttgatct cactaacttt gtgccaggtg ataaaagtgc agcaaggcac gaattaggga
     6841 ttgccgcaaa tgcagttgtg atgctttttg ttgggcgcat tcaaccactt aaagcgccag
     6901 atgttttatt aaaagcagct gctgaattaa ttcgtcatca accagagtta cgtaaccgac
     6961 ttgtcgtgcc gatttgtggt ggcccatctg gaactggatt agctaggcct gatgcacttc
     7021 ataaactagc tgctgaactt ggaatttctg aaaatgtaat ttttgaaccg ccaactaatc
     7081 gcgcacgttt agttacttgg ttacaagcag ctgacgtttg tttgattccg tcgtataacg
     7141 agtcatttgg tttagttgcc ctagaagcgc aagcttgtgg tactccagta attgcggcaa
     7201 atgttggtgg attgccaact gcagtttcaa ataattacag cggtttactt gtggatgggc
     7261 ataatccgca tgaatgggca catgtacttg aaaaatactt tgctgatcaa aagttgcaag
     7321 aaaaattacg tgctggcgcc cttgatcacg cagcaaaatt tagttgggaa gcaacaactg
     7381 aacgacttat cgaagtgtac gaagaggcaa tagattcagc gatagcgcgc aggaacgcct
     7441 aatgatttcg cttgatcaac taacagatta cttagctagc caagatctag ctccgcaaga
     7501 tatttctgac actgcacttg tggttactgt tcaaggcacg aacaaactca aaataaatgt
     7561 ggctatttcg ttaggtcaac atacattgcg cattgaatcc tttgtggcac gaaatcctga
     7621 cgaaaaccat gaaaacgtct accgctggat gttggaagaa aatcgcaaac tacttaccgt
     7681 ggccttttgt ctagatacct ttggtgatat ttatttacgg ggtagtttgc caatcgaaaa
     7741 tttaacgctt agtgatgtcg atcaaatact tggcgtagta attgcccaga gtgatcgcag
     7801 ttttaaccca tgactggaac ttggttttaa aagcgcaat
//
LOCUS       DBNHVN010000179         2804 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1742865, whole genome shotgun sequence.
ACCESSION   DBNHVN010000179 DBNHVN010000000
VERSION     DBNHVN010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1742865"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..279
                     /locus_tag="AC16Y1_03465"
                     /pseudo
     CDS             1..279
                     /locus_tag="AC16Y1_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015036375.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(333..899)
                     /locus_tag="AC16Y1_03470"
     CDS             complement(333..899)
                     /locus_tag="AC16Y1_03470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017219.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR5831552.1"
                     /translation="MKALAFLISATLTFSMGAISGQSSVQAKDAQIRPKVHQAAKPIK
                     NNKASRIKFNPDAAPVSAEAIYVAIAAALTKDDAAYRAGGKGPNSFDCSGLVVWAYAQ
                     AGVSLPHGSNNQLKVVRPIPREELLPGDLIFSGRAGGRIQHVALYIGDGKVIHATYPT
                     ASRANQVRIDDLDGSWVMVHKAYGRVVQ"
     gene            complement(1152..>2804)
                     /gene="leuA"
                     /locus_tag="AC16Y1_03475"
     CDS             complement(1152..>2804)
                     /gene="leuA"
                     /locus_tag="AC16Y1_03475"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR5831553.1"
                     /translation="RTWPNNVITTAPRWCAVDLRDGNQALIDPMTPDRKRRMFELLVK
                     MGYKEIEVGFPSASQTDFDFVRMLIEENLVPDDVVIQVLTQAREHLIERTFEAIEGAP
                     QAIVHLYNSTSTLQRRVVFDLDKDGIKEIAVRGAELCLKYAEQIQDKTDVYFEYSPES
                     FTGTELDYAIEVVNAVNEVWKPTPDRKVIINLPATVEMATPNIYADSIEYMNRKLANR
                     ESIVLSLHPHNDRGTGVAAAELGYMAGADRIEGCLFGNGERTGNVCLVTLGMNLFSQG
                     IDPEIDFSNIDEIRRTVEYCNQLSVPERHPYGGDLVFTAFSGSHQDAINKGLAAMKRD
                     ADAAGVSIDDFKWAVPYLPIDPKDIGRSYEAVIRVNSQSGKGGVAYIMRTEYKLELPR
                     RLQIEFSQVIQRHTDSEGGEVTPAQMWEIFKDEYLPNPDKPWGRFKLKTVAVDSDVEG
                     DTKVRVLMSDTTNTAGEVNLEGIGNGPIAAFANALAQVGVDVRVLDYHEHAMSSGGDA
                     QAAAYLECAVDGRMLWGVGIDSSIVTSSLKAVVSAVNRAIRG"
BASE COUNT          742 a          662 c          627 g          773 t
ORIGIN      
        1 aaacctagcg tgcacattat taatgcagca cgtggtggag tgctcgacga agccgctcta
       61 tataaagcca tttcgcaagg cagagtagcc ggtgctggac ttgatgtata tgcaactgaa
      121 ccatgtattg attcgccatt atttgagctt gatcaagttg tagcgacacc gcatctaggt
      181 gcctcgactg aagaagcgca ggaaaaagca ggcgtcgcag ttgcagagtc tgtggttgcg
      241 ttctttggtg gctatgaagt gccaggtgcg tttaacttgg ctcaattaga aaacgtgtaa
      301 ttagcagaaa gcgcttattg ggtcgagttg tcctattgga caactcgacc ataagccttg
      361 tgcaccataa cccaactacc gtctaaatca tcaattcgta cttgatttgc ccttgatgca
      421 gtcgggtaag tagcgtgaat tactttgccg tcaccgatgt ataaggccac gtgctgtatt
      481 cgaccgccag cgcgaccaga aaaaattaag tcaccaggta gtagttcttc tcgaggaatc
      541 gggcgcacaa ctttgagttg attatttgag ccatggggca gggatacgcc tgcttgagca
      601 tatgcccaga ccaccaatcc tgagcaatca aatgagtttg gtcctttacc gccagcgcgg
      661 taagcagcat cgtctttagt taaagctgcc gcaatggcta cgtatatagc ttcagcagaa
      721 accggagctg catcaggatt gaatttgatg cgtgaagcct tgttattttt aattggctta
      781 gctgcctggt ggactttagg ccggatctgc gcatctttag cttggaccga actttgccca
      841 gaaatggcgc ccattgagaa agtaagggta gcgctgatta gaaaggctaa agctttcact
      901 tttcgatttt gcatattctt ttaaaccttt tgctctcgac aagttcttgc tttggtttgt
      961 ttaccttttc gaccatacgt cggtacttcg ggcgcaccca aactgcgaaa cgccgaaaac
     1021 cccattggat tagcgaatgt gatttatgcc acttttatat gcgtagcgga actaaaggga
     1081 atataaaaaa gtaaatacag gagttaacca ggaagtggat aggcgcttcg atccttcgct
     1141 tcgctcagtc ctcagccgcg gatggctcta ttcactgctg acacaactgc ctttagggag
     1201 gaagtgacaa tcgaagaatc gattccaaca ccccaaagca tgcgaccatc aacagcacac
     1261 tctaaataag ccgctgcttg cgcatcacca ccactactca tggcatgttc gtggtaatcc
     1321 aaaactcgaa cgtctacacc tacttgagct aaagcatttg cgaatgcagc aattggacca
     1381 ttaccaatgc cttcaagatt tacttcacca gcagtatttg tggtgtccga cataagtacg
     1441 cgaactttgg tatcaccctc gacatcgcta tctacggcaa cagtctttaa tttgaagcga
     1501 ccccaaggtt tatcgggatt tggcagatac tcatccttaa atatctccca catctgtgcc
     1561 ggggttactt cgccaccttc tgaatccgtg tgacgttgaa ttacctgact gaattcaatt
     1621 tgcaggcgcc gaggaagttc gagtttgtat tcggtgcgca taatgtaagc cacaccacct
     1681 ttacccgatt gcgaattaac tcggataact gcttcgtaac tgcggccaat atcttttgga
     1741 tcaattggca agtaggggac agcccactta aagtcatcaa ttgaaactcc cgcagcatcc
     1801 gcatcccgct tcatcgcggc caagccttta tttattgcat cttgatggga acccgagaat
     1861 gcagtgaata ccaagtcacc accatatgga tgacgctctg gaacagaaag ttgattgcaa
     1921 tattcaactg tgcgacgaat ttcatcaata ttactgaaat caatttcagg atctatccct
     1981 tgactaaata aattcatgcc aagggtaacc aagcaaacat ttccggttcg ctctccatta
     2041 ccaaataaac aaccttcaat gcgatcagcg ccggccatgt agccgagttc agctgcagcc
     2101 acaccggtac cgcggtcatt gtgtgggtga agtgacagca caatcgattc gcgattagct
     2161 aattttcgat tcatgtattc gatactgtcg gcataaatat tgggagttgc catttcgacg
     2221 gtagctggca aattgataat tacttttcga tctggagttg gtttccacac ctcgtttact
     2281 gcattaacta cttcaatcgc gtaatcaagt tcggtgccag taaaagattc cggagaatac
     2341 tcaaaataaa catcagtttt gtcttgaatc tgttcggcat attttaagca aagctcagct
     2401 ccccgaactg cgatttcttt aatgccatct ttatcaaggt caaaaactac tcggcgctgc
     2461 aatgttgaag ttgagttata taggtgaact attgcttgtg gcgcaccctc gattgcctca
     2521 aaagtacgtt caattaaatg ctcgcgcgct tgggtaagta cttggattac tacgtcatct
     2581 ggaacaaggt tttcttcgat tagcatgcga acaaaatcaa agtcagtttg gctagccgaa
     2641 gggaaaccta cttcaatttc tttgtagccc atttttacta gcaactcgaa cattcggcgt
     2701 ttgcggtcag gggtcattgg atcaatcagt gcctggtttc catcgcgtaa atccacggcg
     2761 caccatcttg gcgccgtagt aataacgttg tttggccagg tgcg
//
LOCUS       DBNHVN010000180         1275 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_479188, whole genome shotgun sequence.
ACCESSION   DBNHVN010000180 DBNHVN010000000
VERSION     DBNHVN010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1275)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_479188"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="AC16Y1_03480"
     CDS             complement(<1..217)
                     /locus_tag="AC16Y1_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017579548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase J family beta-CASP ribonuclease"
                     /protein_id="HIR5831554.1"
                     /translation="MTHAHPELGRPPALADGHLRIVALGGLGEIGRNMTVLEFNGRLL
                     IVDCGVLFPEESQPGVDLILPDFDYIRD"
     gene            complement(214..1113)
                     /gene="dapA"
                     /locus_tag="AC16Y1_03485"
     CDS             complement(214..1113)
                     /gene="dapA"
                     /locus_tag="AC16Y1_03485"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018521480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR5831555.1"
                     /translation="MSAAKDAFGTVLTAMVTPFTKDGLKVDLAAVEKLANDLVELGNN
                     GIVVNGTTGESPTTDEHEKLEIIKAVKSAVGDRVKVVAGAGSNDTAHSVLLAKDAAKA
                     GADGLLVVTPYYNKPPQDGIIAHMQAVADSTDLPVMVYDIPGRAGVPITTESLLKLSE
                     HPKILANKDAKGDPWASSVVMAKSDLAYYSGDDGLNLALLAIGAVGVVSVTGHIVADR
                     HKQMVEAVAANDIGKAQKINEGLVPITEGIMTRVGGAIMVKAALDLLGRAGGGFLRLP
                     LIPATASQREVLRADLIAGGFSL"
     gene            complement(1127..>1275)
                     /locus_tag="AC16Y1_03490"
     CDS             complement(1127..>1275)
                     /locus_tag="AC16Y1_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015490672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HIR5831556.1"
                     /translation="LRRKAEGSHFPSFPQREIEMVAEKYEEVFKQLMPLTHAAFVANG
                     RVAP"
BASE COUNT          334 a          326 c          269 g          346 t
ORIGIN      
        1 gatcccggat gtaatcaaag tcaggcaaaa ttaaatcaac accaggttga gattcctctg
       61 gaaacaaaac accacagtca acaattaaca ggcggccatt aaattcgagc accgtcatat
      121 ttcgaccaat ttcgcccagt ccaccaaggg ctacgatgcg caagtgccca tcagctaatg
      181 ctggtggtct gcctaactcg ggatgggcgt gggtcacagg ctaaaaccgc cagcaatcag
      241 atcagctcgt aaaacttctc gctgacttgc tgttgctgga attaacggaa gtcgcaagaa
      301 gccgccgccg gctcgaccaa gtaaatctaa tgctgcttta accatgatgg caccgccgac
      361 tcgagtcata atgccttcgg ttatcggcac aagtccttcg ttaatttttt gtgcctttcc
      421 aatatcgttg gccgcaacag cttcaaccat ttgtttgtgt cgatcagcga ctatgtgccc
      481 ggttacgctg actacgccaa ctgcaccaat tgcaagtaaa gctagattca atccgtcatc
      541 acctgagtaa taggccaagt ctgacttagc catgacaaca gaagaagccc acggatcgcc
      601 ctttgcatct ttgttagcca aaatttttgg atgctcagac agtttcagta atgactcagt
      661 tgtgattgga actccggcac ggcctgggat gtcgtaaacc attactggca aatctgtgct
      721 gtctgctacg gcttgcatat gcgcaataat tccatcttgc ggaggcttgt tgtaataagg
      781 agtaactact agcaatccat cagcgccagc tttggcagca tccttggcca gtaaaactga
      841 atgagcagtg tcatttgatc cagccccagc aacaactttt actcggtcac caactgcaga
      901 tttaactgct ttgataatct caagtttttc gtgctcatca gtagttggcg attcccctgt
      961 tgtgccgtta acgacaattc cattattacc aagttcaact aaatcattcg ctagcttttc
     1021 aacagctgcc aaatcaactt tcagcccatc tttagtaaat ggggtgacca ttgcggttaa
     1081 gactgtgcca aacgcatctt ttgctgcgga catcttcact cttttcttat ggtgctactc
     1141 gaccatttgc cacaaatgct gcatgagtta acggcataag ttgcttgaat acttcttcat
     1201 acttttcggc gaccatttca atttcacgct gtgggaaaga tggaaaatgg gaaccttctg
     1261 ccttgcggcg caaac
//
LOCUS       DBNHVN010000181         2031 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1074210, whole genome shotgun sequence.
ACCESSION   DBNHVN010000181 DBNHVN010000000
VERSION     DBNHVN010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1074210"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            232..936
                     /locus_tag="AC16Y1_03495"
     CDS             232..936
                     /locus_tag="AC16Y1_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010351073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIR5831557.1"
                     /translation="MSVSPVVKTAQGVRGGIPEATVNRLPTYHRSLLALASRGVLLVS
                     SEELADVSGVTSTKLRKDLSFLGSYGTRGVGYEVEFLLYQIARALGLTQQWPVVIVGI
                     GNLGHALAGYQGFASRGFNVVGLFDSDNSRVGEKVSVAGNSLVVNSLTDLPRVVRMSG
                     ASIGVIATPENSAQEVCDVLVANGVTSILNFAPVLLDVPAGVDVRRVDLATELQILAF
                     HEQRKSQLVLSESNAG"
     gene            939..>2031
                     /locus_tag="AC16Y1_03500"
     CDS             939..>2031
                     /locus_tag="AC16Y1_03500"
                     /EC_number="2.1.1.107"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF012799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional uroporphyrinogen-III
                     C-methyltransferase/uroporphyrinogen-III synthase"
                     /protein_id="HIR5831558.1"
                     /translation="MTEQIVSAIEENNLADSEITETNKVGMGMASPATAAVGVSFVVA
                     GPGDADLLTVRAHRLLSQADVVLVDPDLVPLAEKVSAGQILISVDANGVPMSLAARTK
                     AICDANKIGQQVVRLLSGDPILDGRLALETAALADADLNFEVAAGVSALTAVPAHLGM
                     SLTGGRTREVRILDADVPGINWTVHTDPFVTLVVHNAADRAAEISKSLIKAGRDAKTP
                     VSIIRDGATVEQRSLHSTLEQLPAAVKLSKQAGQGLVIIGEAASERTSWYENRPLHGW
                     RVLVPKTKDSTIDLCEQLVTFGAVPFEVPTMSVEPPRTPQQMERAVHGLVSGRFEWVA
                     FTSVNAVRAIRERFAEYGLDARSLSGIRIA"
BASE COUNT          540 a          423 c          499 g          569 t
ORIGIN      
        1 gttgttctag tcgatggaaa gcatcatgac caatggtcag tagacccaga gcgtcttaaa
       61 aaagccttaa gtctcgggat ttaatttttt cccaattcac gttagtcaca cgagtaacac
      121 gataaaaaaa cgtcatcgca ctatcacgga tataggcata tatgccaatt ttgcctagcg
      181 ccgatatgcg cctaatctga tcgccaagag cacgaaaaaa ggttggttcg aatgtcggtg
      241 tcacccgttg tgaaaacagc acaaggtgtt cgcggaggca tccccgaagc aacggtaaat
      301 cgtctaccga cgtaccacag atcgttactt gcattagcaa gtcgaggcgt acttttagtt
      361 tcgtccgaag aacttgcaga cgtatcaggt gtaactagca ccaaacttcg taaagattta
      421 tctttcttag gttcgtatgg aactcgtggc gttggctacg aagtggaatt tctgttgtat
      481 caaattgctc gcgcattagg tcttactcaa cagtggccag ttgtaattgt cggaatcgga
      541 aatcttggtc acgctttagc tggctatcaa ggttttgctt cacgcggttt caacgtagtg
      601 ggattatttg attcagataa ttctcgcgtt ggtgaaaaag tttcagttgc tggaaattct
      661 ttagttgtta actcattaac tgatttacca agagttgttc gaatgagtgg agcatcaatt
      721 ggtgttattg caacgccaga aaattctgcg caagaagttt gtgatgtgct ggttgctaac
      781 ggggtaacca gcattcttaa tttcgctcca gttctacttg atgttccagc cggagtcgat
      841 gtacgtcgcg ttgatttagc gactgaatta caaattttgg catttcacga acaacgcaag
      901 tcccaacttg tgttgtctga atcgaatgct ggataagtat gaccgaacaa atagttagcg
      961 ccattgagga aaacaatctt gcggacagcg agattaccga aacaaacaag gtaggtatgg
     1021 gaatggcatc accggctact gcagctgttg gagtctcatt tgtagtcgcg ggccccggcg
     1081 acgctgattt attgacggtg cgagcgcacc ggctattaag tcaggcagat gtagttttgg
     1141 ttgatcctga tttagttccg ttagcggaaa aagtttcagc cggtcagatt ttaatttccg
     1201 ttgatgcaaa tggtgtgcca atgtcgttgg cagcaagaac taaagcaatt tgtgatgcaa
     1261 acaaaatagg ccaacaagtc gttcggttac tttctggcga tcctattctt gatggtcgct
     1321 tagctttaga gactgcagcg ctggcagacg ctgacttaaa ttttgaagtt gctgcaggcg
     1381 tttcagcatt aaccgcagta cctgcgcacc tgggcatgtc gttaactggt ggccgcactc
     1441 gtgaagtgcg catccttgat gcagatgtac ctggaattaa ttggacagtt cacacagatc
     1501 catttgtaac tttagttgta cataacgccg ctgatcgtgc tgcagaaatt agcaaatcat
     1561 taattaaggc tggtcgcgat gcaaaaactc cagtgtcaat tattcgagat ggcgcaactg
     1621 ttgaacaacg ttcacttcac tcaacacttg agcaacttcc agcggcagtt aaattgtcaa
     1681 agcaggctgg tcaaggttta gtaattattg gtgaagctgc cagtgaaaga acatcttggt
     1741 atgaaaatcg gccgcttcac ggctggcggg ttctggtgcc aaaaacaaaa gattcaacaa
     1801 ttgacttgtg cgagcaatta gttacttttg gcgctgtgcc atttgaagtt ccgacaatgt
     1861 ccgttgaacc accacgtacc ccacaacaaa tggagcgtgc ggtacacgga ttagtttcgg
     1921 gcaggttcga atgggttgcg tttaccagcg tcaatgcagt cagagcaata cgtgaacgtt
     1981 tcgcggaata cggtctagat gcaaggtcac tttctggaat tcgaattgct g
//
LOCUS       DBNHVN010000182         1131 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1743271, whole genome shotgun sequence.
ACCESSION   DBNHVN010000182 DBNHVN010000000
VERSION     DBNHVN010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1131)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1131)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1131
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1743271"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1131)
                     /locus_tag="AC16Y1_03505"
     CDS             complement(<1..>1131)
                     /locus_tag="AC16Y1_03505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR5831559.1"
                     /translation="SCQRRVEKALTKIDGVSATVNYATGMAVVETDQPVDSAKLIQAI
                     ENVGYSASLSEKAVEKYGLAEFKQRLIFSSFLTVPLMVLAMVPSLQFDYWQWVCAVLA
                     TPVATWAAWPFHRASFMNFRHRAVTMDTLVSIGVSASYLWSLYAILFTHAGKPDMRMS
                     MSLINTSGSHSAEIYFEVAAAVTTLVILGKFLEYRARNQSIKALENLATLNPKSAILM
                     VEGKQTTVPIEQVKVGDELYVQTGGQIPVDSVVVSGTGHVDKSLVTGESMPVEVTAGD
                     TVIGATVLLDGVLTLRAQAVGKDTVLSGISRLVHQAQAGKAEVTRLVDRVSEIFVPLV
                     ILLSVATGLYWLIIQDNSNLALTASIAVLVIACPCALGLATPT"
BASE COUNT          335 a          275 c          240 g          281 t
ORIGIN      
        1 agttggtgta gctaaaccaa gtgcgcatgg gcaagcaata actaaaaccg caattgatgc
       61 ggtgagtgcc aaattggaat tatcttgaat gattaaccaa tagagccctg ttgcgacgga
      121 tagcaaaata actaacggca caaatatttc tgagacacga tcaaccaatc gagtaacttc
      181 agctttacct gcttgcgctt gatgaactaa gcgtgaaatt cctgagagca cggtatcttt
      241 gccaaccgcc tgtgcacgca gagttaatac gccatctaat aaaactgttg caccgataac
      301 agtgtcgcca gctgtaactt caactggcat cgattcgcca gtcactaaag atttatcaac
      361 atgacccgtg cctgaaacaa ccacgctatc gactggaatc tgtccgccgg tttgcacata
      421 tagttcatcg ccaactttta cttgctcaat tggcaccgtg gtttgcttac cttcgaccat
      481 caaaattgcg gactttggat ttaacgtagc taagttttct aacgctttaa ttgattgatt
      541 gcgagctcga tattctaaaa actttcctaa aatcaccaaa gtagttactg cagcggccac
      601 ttcgaaataa atctcggcgc tgtggctgcc actggtatta attagcgaca tagacattcg
      661 catgtcaggt ttaccggcat gcgtgaacaa aattgcatag agcgaccaaa gataggaagc
      721 actaacgcca atactgacca aggtgtccat cgtgacagcg cgatggcgaa agttcataaa
      781 cgaagcgcga tggaacggcc aagccgccca agttgcaacc ggagtggcta gtaccgcgca
      841 aacccactgc caataatcaa actgcagact cggcaccatg gctaacacca ttaatggcac
      901 agtgaggaaa cttgaaaaaa taaggcgttg tttaaattct gccaagccat atttctcaac
      961 ggctttttca ctaagcgaag cgctgtaacc aacattctca attgcttgaa ttaacttggc
     1021 actatcaact ggctgatcag tttcgaccac ggccatgccg gtggcgtaat taaccgtggc
     1081 agagacgcca tcaatttttg ttaaagcttt ttcaactcga cgctggcacg a
//
LOCUS       DBNHVN010000183         3907 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_776883, whole genome shotgun sequence.
ACCESSION   DBNHVN010000183 DBNHVN010000000
VERSION     DBNHVN010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_776883"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..2046)
                     /gene="tkt"
                     /locus_tag="AC16Y1_03510"
     CDS             complement(1..2046)
                     /gene="tkt"
                     /locus_tag="AC16Y1_03510"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR5831560.1"
                     /translation="MSDFNWTPLDDQAVVTARALAMDSVQKVGNGHPGTAMSLAPMAY
                     LLFQKWLNHDPSDSTWLGRDRFVLSNGHSSLTLYIQLYLSGYKLELDDLKSFRTWNSK
                     TPGHPEFGHTDGVETTTGPLGTGVATAVGMAMATRYVRGLLDPQAPAGTSPFDHRVWV
                     IAGDGCLQEGVSAEASSLAGHQKLGNLVMLYDDNHISIEGDTNVSFSEDVLARYQAYG
                     WNVDHVDMNSDGSINVAKLDEAIARVVTVTDRPSIIRVRNIIGWPAPNLKNTGKIHGS
                     ALGEAEVAASKELLGLDSNLSFVVPDEVISHTRKVRDRGKLAHQAWDKKLANWKESNP
                     EGSKLFDRLVSGKLPADFEKAIPKFEVGKEVATRKASGDVINAIASVLPEFWGGSADL
                     GESNLTTIEHGKSFLPADSKMPDASPSGRILHFGIREFAMGAILNGIALSGLLRPFGG
                     TFLVFSDFMRGSVRLAALMNLPVKYVWTHDSIGLGEDGPTHQPIEHLWALRAIPNLDV
                     TRPADANETSAVWQIALNRQNPVALCLSRQNLPVLDTSATSDLSKVSKGAYVIKEAST
                     TPKAIVLASGSEVYIAIDAANQLEQSGIATRVISVPCLEWFEEQSEQYKNELLPASVT
                     NRVSIEAGIAQGWWKYVGSSGRCISIEHFGASASAGKLYQEFGITAQAVIEAVKDGQ"
     gene            2166..3041
                     /locus_tag="AC16Y1_03515"
     CDS             2166..3041
                     /locus_tag="AC16Y1_03515"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR5831561.1"
                     /translation="MLPTWRAYLALTKPRIIELLLITTIPTMFLAAGGFPSFSLMIWT
                     LIGGSLAAGGANSLNCYLDRDIDAIMQRTSHRPSATGQIAPYKIILFGLTLSFLSFVV
                     LGLAVNPLSAFLATVAILFYVVGYTMILKRRTSQNIVWGGAAGCMPVLIGWSAVTNSL
                     NWTPVILFLIVFFWTPPHYWPLSLRYKDDYAAANVPMLPVEETPQVVAKQIVIYGFVM
                     VATSLLLIPVAQMNWLYSVAAVGTGIWFMQEAFKLQKRVKENAAELHPMRLFHLSISY
                     LSILFLAVGLDPFLG"
     gene            complement(3038..>3907)
                     /locus_tag="AC16Y1_03520"
     CDS             complement(3038..>3907)
                     /locus_tag="AC16Y1_03520"
                     /EC_number="1.17.99.9"
                     /inference="COORDINATES: protein motif:HMM:NF014665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR5831562.1"
                     /translation="RNLKKILLANVFAQVGIVITGGLVRVTASGLGCPTWPECVDGSI
                     TPTSAQTESWHKYVEFGNRLLTFVLLIVAILVVLAIRAAKLRRRDLNILSFAIIGGIF
                     AQAILGGITVLTKLHPLTVAAHFLVSIGLITIAYNLYWRASESGYKSFSLNTPELFRR
                     GTQLHTLLALLVIVVGTLVTGSGPHAGDSGDVERLPLDPRNITWLHADLVFLYIGLTI
                     GLFIAARALNLSTKISNAVKLVLLFSAAQGLIGYIQYFTGLPWVLVAFHIAGSCALWI
                     VTLRLQYSTRTLS"
BASE COUNT         1079 a          923 c          839 g         1066 t
ORIGIN      
        1 ttactggcca tcctttactg cttcgattac cgcttgtgcg gtaatcccaa actcttggta
       61 caacttgcca gcactggctg acgcaccaaa atgctcgata cttatacaac gtcctgagga
      121 gcccacgtat ttccaccagc cctgagcgat tccagcttca atcgaaaccc gattagtaac
      181 tgatgcaggt agtaattcat ttttgtattg ctcggattgc tcttcgaacc actcaagaca
      241 aggcactgag atcactcgcg tagcaatacc actttgctca agttggtttg ctgcatcgat
      301 tgcaatgtac acctcgctac cggaagccaa cacaattgct tttggcgttg tacttgcttc
      361 cttaatgacg taggcacctt tactaacctt ggataagtca gatgtcgccg atgtatctag
      421 gactggcaga ttttgtcttg ataaacaaag tgctactgga ttttgtcgat tcagtgcgat
      481 ctgccatacc gctgaggttt catttgcatc tgcaggccta gttacgtcta agttggggat
      541 cgcgcgaagc gcccacaagt gttcgattgg ttgatgcgtt ggaccatctt caccaagacc
      601 aattgaatcg tgggtccaaa catatttaac gggcaaattc attaacgcag ctagtcggac
      661 tgaaccacgc atgaaatcac taaagactaa aaatgtgcca ccgaatggcc ggagtaaacc
      721 acttaaagca attccgttca gaattgcacc catggcaaat tctcgaattc caaaatgcaa
      781 aatacgacca gaaggactcg catctggcat tttcgaatca gccggtaaga aacttttacc
      841 atgctcgatg gtggtcaaat tactttcgcc caaatcggct gaaccacccc aaaattcagg
      901 taagaccgaa gcgatcgcat taataacatc accggaagct ttacgagtag caacttcctt
      961 tccaacttca aatttaggaa ttgccttttc aaagtcagcg ggtaacttac cgctaaccaa
     1021 tcggtcaaat aattttgatc cttcaggatt actttctttc cagttggcta actttttatc
     1081 ccaagcctgg tgggctaact tacctcggtc acgaacttta cgcgtatgtg aaataacttc
     1141 atctggaact acaaaactta agttcgaatc aagtccaagt aattctttac ttgcggcaac
     1201 ttcagcctcg cccaaagctg agccatgtat tttgccagtg ttttttaggt ttggcgctgg
     1261 ccaaccaatg atatttcgta ctcgaataat cgatggtcga tcggtgactg tgaccacgcg
     1321 agcaatcgct tcatctaatt tggcaacatt aatacttcca tcagagttca tgtcaacgtg
     1381 atctacattc cagccataag cctggtaacg agctaagaca tcttcactga aggaaacgtt
     1441 tgtgtcgccc tcaatcgata tatgattatc gtcatagagc atcactaaat tgcctagttt
     1501 ctgatggcca gcaagtgaag aagcctctgc ggaaacacct tcttgtaaac atccatcccc
     1561 agcaataacc cagacccgat ggtcgaatgg gctggtgccg gccggagctt gcggatcaag
     1621 taatccgcga acataacgtg tggccattgc catcccaaca gcagttgcca ctccagtacc
     1681 taacgggcct gtagttgtct cgacaccgtc agtgtgacca aactctggat gtcctggagt
     1741 ttttgaattc caggtccgaa aactttttaa atcatcaagt tctaatttgt aaccagaaag
     1801 ataaagctga atgtaaagag tcaggctgct atgcccattt gaaagaacaa accgatcccg
     1861 acctaaccaa gttgaatcac ttggatcgtg atttaaccat ttttggaata gtaagtaagc
     1921 cataggtgcc aggctcattg ctgtacctgg atggccatta cctacttttt ggacactgtc
     1981 catggctaaa gctcgggcgg taactaccgc ttggtcatct aatggggtcc agttgaaatc
     2041 actcacgtaa taagggtatc cagtgctctc agcagttggc ttgagtatcc agttgttttg
     2101 ccacaggtag gctaaatgat gtgtcagtta ccgcgtcggg ttccgcagca gaagcaagtg
     2161 gctttattct gccaacttgg cgggcatatt tagcactgac aaagcccaga atcatagaac
     2221 tacttttaat caccacgatc ccaacaatgt ttctagctgc tggtggattt ccgtcattct
     2281 cattgatgat ttggacctta attggcggct cacttgctgc tgggggagca aattccctca
     2341 actgctacct tgatcgcgac attgatgcaa ttatgcagcg cacatcgcat cgaccctctg
     2401 ctactgggca aatcgcacct tacaaaataa ttctttttgg gttgaccctg tcatttttgt
     2461 catttgtggt cttaggttta gcagttaatc cactttcagc atttttggct acggtagcaa
     2521 ttttgtttta tgtagttggc tacaccatga ttttgaagcg tcgcacttcg caaaacattg
     2581 tctggggtgg ggcagccggt tgtatgccag tgctaattgg ttggtcagca gtgacgaatt
     2641 cactgaactg gacaccggtg atacttttcc taattgtgtt tttttggaca ccgccacact
     2701 attggccact tagtttgcga tataaagatg attacgctgc tgcgaatgtt ccgatgttgc
     2761 cagtagaaga aacaccacaa gtcgtggcaa aacagattgt tatttatggc tttgtcatgg
     2821 tggctacctc actgttactt attccagtgg cccagatgaa ttggctttat tcagtagccg
     2881 cagttggtac cggtatctgg tttatgcagg aagctttcaa attacaaaaa cgtgtcaagg
     2941 aaaatgcagc agagttacat ccaatgcggc tatttcactt gtcgatctcg tatctgtcca
     3001 tactgtttct ggcagtaggc ctagatcctt ttttaggtta gctcagagtt cgagttgaat
     3061 actgcaaacg cagggtaaca atccaaagcg cacatgatcc ggcgatgtga aacgccacca
     3121 atacccatgg caaaccagtg aagtattgaa tgtatccaat caaaccttgt gctgcactga
     3181 ataacaaaac aagtttcaca gcgttagata ttttggtcga tagattaaga gcacgagcag
     3241 caatgaataa cccaattgtt aagccaatgt aaagaaaaac taaatcggca tgtagccaag
     3301 ttatgtttct tggatcaagt ggtaatcgct caacatcacc tgagtctcca gcatgtggcc
     3361 cagaaccagt gacaagtgtg ccaaccacaa ttacaagtag ggcaagcaaa gtgtggagtt
     3421 gagtaccgcg cctaaataac tcaggcgtat ttagtgagaa tgatttgtag cctgactcac
     3481 tagcccgcca ataaaggttg tacgcaattg tgatcaatcc gattgagact aaaaagtgtg
     3541 cagcaacagt caacggatgc aacttagtaa gaactgtgat tccacccaaa attgcttggg
     3601 caaaaatccc accaattatt gcaaaagaca aaatattcaa gtcacgccga cgaagttttg
     3661 cggctcgaat tgctaacaca accaaaatcg caacaatcaa taaaacgaaa gtgagtagcc
     3721 ggtttccgaa ctcaacgtat ttatgccatg attcggtctg cgctgaggtt ggcgtgattg
     3781 agccatccac acattctggc caagtagggc aacccaggcc agaagcagta actcgaacta
     3841 atccacccgt gatcacgatg ccaacctgcg cgaaaacgtt agccagcagg atctttttca
     3901 ggtttcg
//
LOCUS       DBNHVN010000184         1985 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_479675, whole genome shotgun sequence.
ACCESSION   DBNHVN010000184 DBNHVN010000000
VERSION     DBNHVN010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_479675"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="AC16Y1_03525"
     CDS             complement(<1..520)
                     /locus_tag="AC16Y1_03525"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005449491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR5831563.1"
                     /translation="MSELEGIRLQKVLASAGLGSRRKCEEFIIAGRVTVNGEVVDELG
                     SRVNPDVDLIHVDGVRIAQASGQIVVALNKPRGVVTSMQDDQGRECVGDLVAEYPERL
                     FHVGRLDADTEGLLLLTNDGEISQRLSHPSHGIVKTYFARVSGKMTKEQIAQIRAGVV
                     IEGNPVAVTDCLL"
     gene            complement(523..1176)
                     /gene="scpB"
                     /locus_tag="AC16Y1_03530"
     CDS             complement(523..1176)
                     /gene="scpB"
                     /locus_tag="AC16Y1_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR5831564.1"
                     /translation="MSDELESQVEDLGLQDGELATEEETDDGVSLGAPSLPAALEALL
                     LVAEEPVTLATLATLTRTPEDQVLKTLHDLAQEYAIQGRGFDLREVGGGWRFWTRDNC
                     SQVVERFVLDGQQGRLSQAALETLAVVAYRQPVTRGRITAIRGVNVDGVVRTLVTRGL
                     IEESGHDHETGAHLDRTTPYFLERLGLKSLEDLPPIAQHLPMPDTVEEVLDEVNSQG"
     gene            complement(1169..>1985)
                     /locus_tag="AC16Y1_03535"
     CDS             complement(1169..>1985)
                     /locus_tag="AC16Y1_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR5831565.1"
                     /translation="GPFDVLLSLIAKHRLDVTALALHTVTDDFIKYIKERGDEWSLDE
                     ATEFLVIAATLLDLKAARLLPGGEVEDEEDIAILEARDLLLARLLQYRAFKQMSADFR
                     HLLATAGRMRPRTVGLDERFLKLLPEVEINLSPDDFANLAARALEPKLPEVISMAHLH
                     AAKVSVREQAAIIVERLRRLGQATFRQLTVDCDTTLLIVARFLALLEIYRDGNVMFDQ
                     PTPLGELYVRWVGSENEEAIMRDIDEFETTIDEFAVEQVQQFRDAADWVTEDE"
BASE COUNT          539 a          517 c          396 g          533 t
ORIGIN      
        1 gtaaaaggca gtcggtgacc gccaccggat taccttcgat gactactcca gctcttatct
       61 gagcaatctg ttcttttgtc attttgcccg atactcgagc aaagtaagtt ttcacaattc
      121 catgtgatgg atgactaaga cgctggctta tttcaccgtc attcgtaagt agtaataggc
      181 cttcagtatc tgcatctaag cggccgacgt gaaataaacg ttctggatac tcggcaacta
      241 aatcgcccac acattcgcga ccttgatcat cttgcatcga tgtcaccacg cccctaggtt
      301 tatttagcgc caccacaatc tgaccgctag cttgtgcgat gcgcacgcca tccacgtgaa
      361 tcaaatcgac atctggattt acccgcgaac cgagttcatc aacaacttca ccatttacgg
      421 ttactcgtcc ggcaataata aattcttcac atttacggcg tgaacccaaa ccggcacttg
      481 ctagaacttt ttgaagtcga ataccttcga gttcactcat aattagccct gactatttac
      541 ttcgtccaga acttcttcga cagtatctgg cattggaaga tgctgagcta tcggtggtaa
      601 gtcttcaagt gacttaaggc caagtcgttc taagaaatat ggcgtcgtgc gatctaaatg
      661 tgcgccagtt tcatgatcgt gaccactttc ttcaattaat ccacgagtta ccaatgtacg
      721 caccacacca tcaacgttca cgccacggat tgctgtaatg cgaccgcgcg taactggctg
      781 tcggtatgca acgacggcca atgtctcgag cgcagcttgg cttaatcgtc cttgctgacc
      841 atcaagtacg aaacgttcaa caacttgtga acagttatcg cgtgtccaaa aacgccaacc
      901 gccaccaact tcacgtaaat caaaaccgcg accttgtatt gcatactctt gcgctagatc
      961 gtgcaaagtt tttaaaactt gatcttcagg tgtacgcgtt agtgttgcca aagtagcaag
     1021 tgtcaccggt tcttcggcaa ccaataacaa ggcttcgagt gccgcaggca aacttggcgc
     1081 gcctagtgaa acaccatcat cggtttcttc ctcagtagca agttcaccgt cttgtaaccc
     1141 taaatcttca acttgtgact ctaattcatc actcatcttc agttacccaa tctgctgcat
     1201 cacggaattg ctgtacttgt tctactgcaa actcatcaat agttgtttca aactcatcta
     1261 tgtcgcgcat aatcgcttct tcgttttcac tacccaccca acggacataa agttcaccca
     1321 atggggtcgg ttgatcaaac atcacattgc catcacgata aatttcaagc agcgctaaaa
     1381 atctggccac aatcaaaagc gtagtgtcac aatcaacagt caattgtcga aatgttgctt
     1441 gtcctaagcg ccgcaagcgt tccacaataa ttgcagcctg ttcacgtacc gaaacttttg
     1501 ccgcatgtaa gtgcgccatg gaaataactt ccggaagttt aggttccagt gcgcgcgccg
     1561 ctaagttagc aaagtcatct ggtgaaagat ttatttcaac ttcaggtaat agttttaaga
     1621 aacgctcatc taaaccaact gtgcgcggtc gcatccgacc cgcggtagcc aataaatgtc
     1681 taaagtcagc cgacatctgc ttgaaagcgc gatactgcaa caacctggca agtaataagt
     1741 cgcgggcttc cagaattgca atatcttctt cgtcttcaac ttccccacct ggaagtaatc
     1801 tggcagcttt taagtcaaga agtgttgcgg caatcactaa gaattcagtt gcttcatcta
     1861 acgaccactc atcgccacgt tctttaatgt atttaataaa gtcatcggta actgtgtgca
     1921 gggcaagcgc agttacatca aggcgatgtt tggcgataag cgacaagagg acatcaaaag
     1981 gtcct
//
LOCUS       DBNHVN010000185         1956 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_181084, whole genome shotgun sequence.
ACCESSION   DBNHVN010000185 DBNHVN010000000
VERSION     DBNHVN010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_181084"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="AC16Y1_03540"
     CDS             complement(<1..670)
                     /locus_tag="AC16Y1_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR5831566.1"
                     /translation="MAAILALLSSFAWGTADFLGGILSKKRNAVAVIGGSQPFGLIAI
                     TIVALLAGQFWFSREVVFYGVVAGLIGMVALMAYYQALATGQMGIVAPIGSLGVLVPL
                     TYGYLDGEQPTSIQNLGIAIAIIGVVLASGPEFRGDAAIKPVVLAFISALMFGFTVLF
                     MARGGELNPAGTVVMMRVAQVSVMVSVALLLRTFGGLKKQDIPILALTGSLDAVANVL
                     FTTAA"
     gene            778..1353
                     /locus_tag="AC16Y1_03545"
     CDS             778..1353
                     /locus_tag="AC16Y1_03545"
                     /inference="COORDINATES: protein motif:HMM:NF022891.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3000 domain-containing protein"
                     /protein_id="HIR5831567.1"
                     /translation="MKLLTTEGEPMDIWEQVVSSLSNLELRPEFLIEEVPAPARLAPH
                     SFALALDVVDADDKDLANGRFVVLYDPAGQETWNGNIRIVTFVRAPIEKDVVTDELFD
                     DVAWSWLIEALKDSINGFHDISGTVTRTISKSYAGIEDRELSADVEVRASWTPEGADI
                     GEQLTSWLSFVEQAAGLAPLPPGISAISRNR"
     gene            1357..>1956
                     /locus_tag="AC16Y1_03550"
     CDS             1357..>1956
                     /locus_tag="AC16Y1_03550"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006591280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HIR5831568.1"
                     /translation="MTETDTQFTRIEIPREGLPDLIDSPRGLNRAAELLAKGSGPVAL
                     DAERASGFKYSQRAYLVQLRRENSGTFLIDPTEFENLALIQEAISGTDWILHAASQDL
                     VCLAEVGLRPTKELFDTELAGRLLGLPKVGLGTLVETHLGFSLAKEHSAADWSVRPLP
                     DEWLAYAALDVEFLIELWDLLQIQLNEQNKFIWAQQEFEH"
BASE COUNT          568 a          417 c          458 g          513 t
ORIGIN      
        1 cggctgcagt agtgaatagg acattcgcta ctgcgtctag cgaacctgtt aacgcaagga
       61 ttggaatgtc ttgtttcttt aatccaccga aagttctgag taatagtgca acgctaacca
      121 ttaccgaaac ttgagcaact cgcatcatca ctacggtgcc agctggattt aattctccac
      181 cacgagccat aaatagaact gtgaagccaa acatcaaggc ggatataaag gccagtacta
      241 ctggtttgat tgccgcgtca ccacgaaatt ctggaccgct ggccaacacc acaccgatta
      301 tggcaatagc aatgccaaga ttttgaattg aagttggttg ctcaccgtct aaatatccat
      361 aagtaagcgg aaccaaaaca cctagagagc caattggggc tactattccc atttgaccag
      421 ttgccagtgc ttggtagtag gccatcagtg cgaccatgcc gattaatccg gcaacaactc
      481 cgtaaaagac aacttcccga ctaaaccaaa actgaccagc aagtaacgca acaattgtta
      541 tggcgatcaa gccaaaaggc tgggagcccc cgattactgc aactgcatta cgttttttag
      601 agagaattcc acccaaaaaa tcggctgtgc cccaggcgaa gctagaaagt aacgcaagta
      661 ttgcagccat ccctgaactc tatcggttta cgttaactga ccattaatct cttaacgctt
      721 aataattagc ggcgcgccaa ggcatagcgc tcaagcaata tttataggct taaaggaatg
      781 aaactactta ctactgaagg tgagcccatg gatatttggg aacaagtagt aagtagcctt
      841 tcgaatttgg aattgcgtcc tgagttttta atagaggaag ttcccgcgcc ggctaggttg
      901 gctccgcaca gttttgctct agccctcgat gttgttgatg cagacgataa agatttggct
      961 aatggccggt ttgtagtttt gtatgaccca gctggccaag aaacttggaa tggcaacatc
     1021 cgaatagtta catttgttag agcaccgatt gagaaagatg tcgtcacaga cgagttattt
     1081 gacgatgtag cgtggtcttg gctaattgaa gccctaaagg attccataaa tggtttccat
     1141 gacataagtg gcaccgttac gcgaactatt tcaaaatcgt atgccggaat tgaagatcgg
     1201 gaattgagtg ctgatgtgga agttagagca tcttggacac cagagggtgc tgacatcggt
     1261 gaacagttaa caagttggtt aagttttgtg gagcaagcgg cgggtttagc tccattgcca
     1321 ccaggtattt ccgcaatttc tcgaaaccgc taattgatga ctgaaactga tacacaattt
     1381 acgcgaatag aaatcccgcg agaaggctta cccgatttaa ttgatagtcc tcgtggatta
     1441 aatagggccg cagagttact agctaaggga tctgggcctg ttgccttaga tgccgaacgt
     1501 gccagtggct ttaagtacag tcagcgtgcg tatctggttc agcttcgccg agaaaatagc
     1561 ggcacatttc taattgatcc aaccgaattc gagaatttag ctttaattca agaagcgata
     1621 agtggaactg attggatttt acatgcggcg agtcaagatt tagtttgctt agcagaagtc
     1681 ggactaagac ccaccaaaga attatttgat acagaacttg ctggtcgatt actcggttta
     1741 ccaaaagtgg gcttaggaac tttagtcgaa actcaccttg gattctcgct ggccaaagag
     1801 cacagtgcag ctgattggtc ggttaggccg ctgccagatg aatggcttgc ttatgcggca
     1861 ctagatgttg aatttttaat tgaactttgg gatctacttc aaattcagtt aaacgaacaa
     1921 aataaattta tttgggctca gcaagaattt gaacat
//
LOCUS       DBNHVN010000186         2204 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_777408, whole genome shotgun sequence.
ACCESSION   DBNHVN010000186 DBNHVN010000000
VERSION     DBNHVN010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_777408"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="AC16Y1_03555"
     CDS             complement(<1..361)
                     /locus_tag="AC16Y1_03555"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR5831569.1"
                     /translation="MNQALPDKFSMLGLTYDDVLLLPAASDVVPSEVETSTRLTREIT
                     LRIPLVSSAMDTVTESRMAIAMARQGGIGILHRNLSIEDQASQVDLVKRSEAGMVTNP
                     VTCLPDNTLAEVDRLCGR"
     gene            complement(443..1429)
                     /locus_tag="AC16Y1_03560"
     CDS             complement(443..1429)
                     /locus_tag="AC16Y1_03560"
                     /inference="COORDINATES: protein motif:HMM:NF014646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR5831570.1"
                     /translation="MTQHPSNSGSESDLQIVLSVAAVARRLGVAPATLRTWDRRYGLG
                     PSEHATGKNRLYSAADLAKLEKMRSLVMQGVLPGKAAEIALQSLAAEDTLRNFKVTDT
                     NSFTTEIDRETNQSESENVISLSTPKNTVRGLVRAANMLDSTSCTNIIAHEIEANGVV
                     WAWANVIVPALQIVGEKWNETDSGIEVEHLLSEVIESQLRTITGANFEPVNTRPVLLC
                     CTTHELHTLPIYAIAAALATKQISCRILGARLPIDSLLAAAKKIGPSAILVWSQTRGT
                     EEPTLWDQLQDQRPAAVKIAAGPGWTVQLPDDVKQPASLTETLDILTAANGL"
     gene            1550..1849
                     /locus_tag="AC16Y1_03565"
     CDS             1550..1849
                     /locus_tag="AC16Y1_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012795972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HIR5831571.1"
                     /translation="MADVSLLPGPNSDFWDWQLHSACRGLDSANFYHPDGERGPAREN
                     RIARAKAICATCPVLTQCREHAIKTRETYGVWGGLSEEERQQIFEMLDRKKRMIS"
     gene            complement(1901..>2204)
                     /locus_tag="AC16Y1_03570"
     CDS             complement(1901..>2204)
                     /locus_tag="AC16Y1_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018745611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HIR5831572.1"
                     /translation="QAQEQAVRMCRPGGRISFFGGLPKDKPMVTIDSNLVHYKELILA
                     GANGSTPVHNQRALDLMASGKVKVSDLLTHTVSLDRVQDAIDLVLGGEAIKVVVKP"
BASE COUNT          569 a          515 c          486 g          634 t
ORIGIN      
        1 accgcccaca aagtcgatca acttcagcca gagtgttatc tggcaagcaa gtgactggat
       61 ttgtgaccat tccagcttcg ctacgtttaa ctaaatccac ttgggatgct tgatcttcaa
      121 tcgacaagtt tcgatgcaaa attccgatgc caccttggcg ggccatcgcg atcgccatcc
      181 gcgattcagt caccgtatcc atagcggagg aaacaagtgg aatccgaagc gttatttctc
      241 gagttagccg ggtggatgtt tctacttcac ttggtactac atcgctagcg gctggcagta
      301 aaagtacgtc atcatatgtc aggcccagca tggaaaattt gtctggaagt gcctgattca
      361 tagctgcctt tcaattttta cttacctaag attactgcct aaacaatggc gcaattaact
      421 aggtttttta acgaccgtaa tcctaaaggc cgtttgcagc agtgagaata tctagagttt
      481 cggtgaggct ggctggctgt tttacatcat caggtaactg cacagtccat cctggaccag
      541 ccgcaatttt cactgctgct ggacgttgat cttgtagttg atcccaaagg gttggttctt
      601 cagtgccacg agtctgtgac caaaccaaaa ttgcacttgg tccgattttc ttcgccgcag
      661 ctaaaagtga atcaatcgga agtcttgcgc ctaaaatacg acaacttatt tgtttggtgg
      721 ctagcgcggc ggcaatcgca tatatcggaa gtgtgtgaag ttcatgtgtt gtgcaacaga
      781 gaagcactgg ccttgtgttc actggctcaa aattagctcc agttattgtg cgaagttgac
      841 tttcgataac ttctgaaagc aaatgctcta cttcgatgcc gctatcagtt tcgttccact
      901 tttcgccaac tatttgtaat gccggaacta tgacattggc ccaagcccaa acaactccat
      961 tagcttcaat ttcatgcgca ataatgtttg tgcaacttgt tgaatccaac atatttgctg
     1021 ctcggactaa accccgaact gtgttcttag gtgttgatag cgaaattaca ttttcagatt
     1081 cagattgatt ggtttcacga tcaatctcgg ttgtaaacga gttcgtatcc gtgactttaa
     1141 aatttcgtag agtatcttct gctgccaaac tttgcaaggc aatctctgct gccttgcctg
     1201 gcaggacacc ctgcataact aacgatcgca ttttttctaa cttcgctaaa tcggctgcag
     1261 aataaagtcg attcttaccg gtggcatgtt cacttggtcc aagcccgtag cgtcggtccc
     1321 aggtacgcaa ggtggctgga gccacgccga gccgacgtgc tactgcggca accgagagga
     1381 caatctgtag atcagattct gatcctgaat tgctcgggtg ttgcgtcacc cctatatctt
     1441 tgggtaaaag cggacactcc gcactttgaa aaaacccttg aaatcagggg tttaccatgt
     1501 tttcaccaag gtacttgaac aatgtatgaa gcgatcttat gatgaggcta tggctgatgt
     1561 ttcattatta ccaggtccta attcagattt ctgggactgg caattacatt ccgcttgccg
     1621 aggattagat tcagcaaact tctaccatcc agacggcgag cgcggtccag ctcgcgaaaa
     1681 ccgcatcgct cgggctaaag caatttgtgc cacttgcccc gtgctaactc agtgccgtga
     1741 acatgcaatt aaaacccgtg aaacttatgg cgtttggggc ggtcttagcg aagaagagcg
     1801 tcaacaaatc ttcgaaatgt tggatcgcaa aaagcgaatg atttcctaat taaaaaagat
     1861 taaggcccct taaatgtagc taaggggcct tattttttgc ttaaggtttt acaacaactt
     1921 ttatggcttc tccacctaaa actagatcaa ttgcatcttg aactctatct aggctaactg
     1981 tgtgggtaag caagtctgaa acttttactt ttccagaagc cattaaatct aatgcgcgtt
     2041 ggttatgaac aggtgttgag ccatttgctc cagccaaaat tagttctttg taatgaacta
     2101 agttcgaatc aattgtcacc atcggtttat ctttaggtaa gccgccgaaa aaactaatcc
     2161 gtcctcctgg ccgacacatt cgaacggctt gctcttgcgc ttga
//
LOCUS       DBNHVN010000187         2344 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_406039, whole genome shotgun sequence.
ACCESSION   DBNHVN010000187 DBNHVN010000000
VERSION     DBNHVN010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_406039"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="AC16Y1_03575"
     CDS             <1..323
                     /locus_tag="AC16Y1_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the oxygen-independent formation of
                     protoporphyrinogen-IX from coproporphyrinogen-III; Derived
                     by automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR5831573.1"
                     /translation="HAHMDGLRSWMTKHPAAWASQVDAKLAPVAGSETLSELEVANEN
                     IMLGLRLSSGIALEVLSASALVQAQLALAQGLLDPVQFANGQAVLTDAGRLLADGLVS
                     RFWN"
     gene            413..1429
                     /gene="hrcA"
                     /locus_tag="AC16Y1_03580"
     CDS             413..1429
                     /gene="hrcA"
                     /locus_tag="AC16Y1_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR5831574.1"
                     /translation="MEDRKLAVLRAIVEDYIQTNEPVGSKALADRHKLGVSSATIRND
                     MALLEEEGLISAPHTSAGRIPTDAGYRVFVDKLNTIKPLSVGERKAIQTFLDQAVDLD
                     DVMGRTVRLLAQLTKQVALVQYPSLGRSSIRHIELVSAGEFRLLLIVIADTGRIEQRV
                     IESPIALTDDLLIELRNKFNAALDGQLFSEVPNRISNLTEQFATGAKPLVASLSATLM
                     ESVLAQTEERVVVGGTANLTRIGSDFLTSVGPVLEALEEHVVLLRLLGEAAEPDAVTV
                     RIGHENPVEELHSASVVTVGYGSGEKSIAHMGVLGPTRMDYAASMTSVRAVAKYVGKI
                     LMGK"
     gene            1430..>2344
                     /locus_tag="AC16Y1_03585"
     CDS             1430..>2344
                     /locus_tag="AC16Y1_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HIR5831575.1"
                     /translation="MADHYQVLGVSRDSSADEIKKAYRKLARELHPDINPDEGERFKE
                     VTAAYEVLSDPQKRQMYDLGGDPFSSGGFNSAAGFGFGDIVDAFFGGAQRGPRPRTRR
                     GQDALIRIQVTLEEAAFGSTKDLTVDTAVRCTICQGSGTAQGASIVDCSMCHGRGEIQ
                     SVQKSFIGQVMTTRQCPQCQGFGTLNPHPCAECAGDGRVRTRSTVTVNIPPGVEGGTR
                     IQMTGQGEVGPGGGPSGDLYIEIIEVAHSVFQRHGDQLHANLKLPMTAAALGAMLEVE
                     TLDGMQSINVKPGTQSGQTVTIKNFGMPR"
BASE COUNT          645 a          485 c          586 g          628 t
ORIGIN      
        1 cacatgcaca tatggatggt ttacgttcgt ggatgacaaa acatccagct gcttgggcta
       61 gtcaagttga tgcaaagttg gctccagttg ctggcagtga aacacttagt gaactcgaag
      121 ttgcaaacga aaacataatg ctgggcttac gccttagctc gggtattgct cttgaggttt
      181 taagcgcttc ggctcttgta caagcacagt tagcgctagc gcagggacta cttgatccag
      241 ttcagtttgc taacggccaa gcggtgctaa cggatgcggg tcgattatta gccgatggtt
      301 tagttagtag attctggaat taattccatt tctacttgga attatgcgaa gtacatcttt
      361 tctgcagtca cggtaaactg gcactctaag caggcaagtg ctaggggtgg cgatggaaga
      421 ccgtaaatta gcggttttgc gcgccattgt tgaagactac attcaaacaa atgaaccggt
      481 tggttcaaag gcattggcgg atcggcacaa attaggtgtc tcttctgcaa ctatccgcaa
      541 tgacatggcc ctgcttgaag aagaagggtt aattagtgcc ccgcatacca gtgctggtcg
      601 aattcctact gatgctggat atcgagtatt cgtagacaaa ctaaatacga tcaaaccgct
      661 ttcagtaggt gagcggaaag caattcagac atttcttgat caagcagtcg acctagacga
      721 tgtcatgggt agaactgtcc ggctacttgc tcaattaact aagcaggttg cactcgttca
      781 atatccgtct ttaggtcgaa gttctattcg acacattgaa ttagttagtg caggtgaatt
      841 tcgcttactg ctcattgtga ttgctgacac tggacgcatc gaacaacgag taattgagtc
      901 accaatcgcc ctaacagatg atttacttat cgaacttcga aataaattta atgctgcact
      961 tgatggtcaa ctattttcgg aagtaccaaa tcgaatttca aacttaactg aacaatttgc
     1021 aactggcgca aaaccacttg tagctagttt gagtgccaca ttgatggaat cggttttggc
     1081 tcagaccgaa gagcgcgttg tcgttggtgg aacagcaaat ctgactcgaa tcggatctga
     1141 tttcttaacc tcagttggcc cagttcttga ggctctagag gaacatgttg tcttacttcg
     1201 gttactaggt gaagctgcag aacctgacgc agtaaccgta cggattggtc atgaaaatcc
     1261 agttgaagag ctacattctg catccgtcgt tactgtgggt tatggctcag gagaaaaatc
     1321 aattgcacat atgggcgtac tcggtccaac gcgaatggac tatgcagcgt ctatgacctc
     1381 agtgcgtgca gtagcaaaat atgttggcaa gattttgatg ggaaagtaaa tggctgatca
     1441 ttaccaagtt cttggtgtta gcagagattc ttcggctgat gaaataaaaa aggcttaccg
     1501 caaacttgcc cgtgaacttc atccagacat aaatcccgat gaaggggaac ggttcaaaga
     1561 agtaacagca gcctacgaag ttttgtctga tccgcagaag cgacagatgt atgacttggg
     1621 tggagatcca ttttcaagtg gcggattcaa ttcagcggct ggatttggat ttggcgacat
     1681 agttgatgca ttttttggcg gggcacaacg aggaccacgt ccgcggactc gtcgtggcca
     1741 agatgcacta attcgaattc aagtgaccct tgaagaagct gcatttggat caacaaaaga
     1801 tttaacggtc gatactgcag ttcgctgcac aatttgccaa ggttcgggaa cagctcaagg
     1861 tgcttcaata gttgactgct caatgtgtca tggccgcggt gaaattcagt ctgtacagaa
     1921 gtcctttatc ggccaagtaa tgacaactag acaatgtccg caatgtcagg gttttggcac
     1981 cctcaatcca catccatgtg cggagtgtgc cggtgatgga agggtaagaa ctagatcaac
     2041 tgttaccgta aacattccac ctggcgttga gggtggcaca agaattcaaa tgactggtca
     2101 aggtgaagtt ggtccgggcg gcggacctag tggagatttg tacattgaaa ttattgaagt
     2161 cgctcattcc gtatttcaac gtcacggtga ccaacttcat gcaaacttga agttgccaat
     2221 gactgccgca gcacttggtg caatgcttga agttgaaaca cttgatggaa tgcaaagtat
     2281 aaatgttaag ccgggaactc aatctggcca gactgtaacg atcaaaaatt ttggtatgcc
     2341 aagg
//
LOCUS       DBNHVN010000188         1732 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1594536, whole genome shotgun sequence.
ACCESSION   DBNHVN010000188 DBNHVN010000000
VERSION     DBNHVN010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1594536"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..139
                     /locus_tag="AC16Y1_03590"
     CDS             <1..139
                     /locus_tag="AC16Y1_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003936069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HIR5831576.1"
                     /translation="RLVELGYKVRVYVPYGDEWYGYLMRRMAEKPSNLALFVRSLASK
                     N"
     gene            297..503
                     /locus_tag="AC16Y1_03595"
     CDS             297..503
                     /locus_tag="AC16Y1_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5831577.1"
                     /translation="MASQNERPLGEVRFLTVAEVATAMRVSKMTVYRLVHSGELPAVR
                     VGRSYRVPEQAVHTYLQESFVEAG"
     gene            535..627
                     /locus_tag="AC16Y1_03600"
     CDS             535..627
                     /locus_tag="AC16Y1_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007465623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein bS22"
                     /protein_id="HIR5831578.1"
                     /translation="MGSVVKKRRKRMAKKKHRKMLKKTRSKRKK"
     gene            complement(673..1530)
                     /locus_tag="AC16Y1_03605"
     CDS             complement(673..1530)
                     /locus_tag="AC16Y1_03605"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019875066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR5831579.1"
                     /translation="MTSINASANQSVNARVNRFDALPTGSKVGAFFDVDNTIMRGASI
                     YHLARGLFSRKILSAGDLRNYALAQGKFLIAGSENLADMARITESALSFVAGRDVEEV
                     KALADEIYHEIMEAKIWPGTVELAQSHLTAGHQVWLVSAAPIELAELIASKLGLTGAV
                     ATISQIVDGKYTGKLVSEPMHGAQKAQAVARLADENGLDLKNCFAYSDSKNDIPLLSE
                     VGHPTVVNPDSELRAYAKLKNWPIYDYRRERLIKRYAIPAGTTAVALIGAGAGMALAN
                     RRKNKKLSE"
     gene            1607..>1732
                     /locus_tag="AC16Y1_03610"
     CDS             1607..>1732
                     /locus_tag="AC16Y1_03610"
                     /inference="COORDINATES: protein motif:HMM:NF017574.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR5831580.1"
                     /translation="MTLIGKPDCHLCQDAAQIVAKVCAELNVGWSELSILDDPKLA"
BASE COUNT          465 a          386 c          402 g          479 t
ORIGIN      
        1 gcgcttagtt gaacttggtt acaaagttcg cgtttatgtt ccttatggtg acgagtggta
       61 cgggtacttg atgcgccgaa tggccgaaaa gccaagtaac ttggcactgt ttgttcgctc
      121 actggcatcc aaaaactagc ctttctaact tctacccggt ccctcaataa caatttttga
      181 atcgactttc acattggtca ctttcaccac taaagttatg acctacgaac aagtgcgtga
      241 gtaactccgg aggggaagcc ggaagtggga acgcacagaa tggtcacggt ttacagatgg
      301 cttctcaaaa tgaacgacca cttggtgaag ttcgtttctt aactgtcgct gaagttgcga
      361 cagcaatgcg agtctcaaaa atgacggttt atcgcttagt gcacagtggc gaattgccgg
      421 ctgttcgcgt tggacgctct tatcgcgtgc ctgaacaagc agtgcacacc tacttacaag
      481 aatcatttgt tgaagcagga taaaactaaa acttaaaaag aagggtggcc gttagtgggc
      541 tccgtagtta aaaagcgccg caagcggatg gcaaagaaaa aacatcgcaa gatgttaaag
      601 aagacaagat caaagcgtaa aaagtaaata acttaaaact agtaccgaga gtaattttgc
      661 tctcggtatt ttttattcac taagtttttt gtttttgcgt cggttggcca gagccatccc
      721 agctcctgcg ccaatcaaag ctacggcggt agttccagcc ggaatcgcgt atctcttgat
      781 gagacgctca cggcggtaat catagattgg ccagtttttt aattttgcat aagcgcgaag
      841 ttctgaatca gggttaacta cggttgggtg gccgacttcg ctcaagagtg gaatgtcatt
      901 ttttgaatcc gagtacgcaa aacagttttt taaatccaaa ccattttcat cagctagccg
      961 agctacggcc tgagcttttt gggcgccatg cattggctca cttactaatt tcccggtgta
     1021 ttttccatcg acaatttggc tgatagttgc aaccgcccca gtcaatccaa gtttgctggc
     1081 aataagttcg gcaagttcaa ttggagctgc agaaaccaac cagacttgat gaccagcagt
     1141 taagtgagat tgggccaact caacggtgcc gggccaaatt tttgcctcca tgatttcgtg
     1201 gtaaatctcg tccgctaggg ctttaacttc ttcgacatcg cgcccagcca caaatgacaa
     1261 agcactttca gttatgcgtg ccatgtctgc cagattttca gacccagcga ttaagaattt
     1321 cccctgcgcc aaggcgtaat tacgtaaatc acctgcactt agtatcttgc gtgaaaagag
     1381 gcctctagct aagtggtaga tgctggcgcc acgcataatc gtgttatcta cgtcgaaaaa
     1441 agctccgact tttgaccccg ttggcagcgc atcgaaacga ttcacacgtg cattaacgct
     1501 ttggttagca ctggcgttaa tactggtcac atctaatagt ctacttttgc aagactatgc
     1561 caaacttaat tgcagattag ttggtggtta aattttggaa aatcgagtga ctttaattgg
     1621 taaacctgac tgccatcttt gccaagatgc tgcccagatt gtggcaaaag tatgcgctga
     1681 attaaatgtt ggttggtcgg aattgtcaat tttggatgac cctaagttgg cc
//
LOCUS       DBNHVN010000189         1285 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_182338, whole genome shotgun sequence.
ACCESSION   DBNHVN010000189 DBNHVN010000000
VERSION     DBNHVN010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_182338"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..967)
                     /gene="aat"
                     /locus_tag="AC16Y1_03615"
     CDS             complement(230..967)
                     /gene="aat"
                     /locus_tag="AC16Y1_03615"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HIR5831581.1"
                     /translation="MKTGNYDFPDLASVPENVELIAVGQTDSTGQPIFLTPGGTWPKL
                     IADGSYWDSEVLIWAYKNGYFPMPLDAKESPLVISWWSPNPRGVLDPGKIKVSKSLKK
                     SLKKFTFTIDQDFEFVIRNCGSLNRPSGWITQQVIDAYLELHELKFAHSVEVRNSAGK
                     IVGGLYGVEVGGLFAGESMFHLETDASKAALVFLARYLNDGQRRLIDCQWQTDHLASL
                     GVQEISRVSYVAQLTKLLECKPRFSGN"
     gene            complement(964..>1285)
                     /locus_tag="AC16Y1_03620"
     CDS             complement(964..>1285)
                     /locus_tag="AC16Y1_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019868969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR5831582.1"
                     /translation="RVTTLGKNGVHIQSKDGLDLRVPVAQEKQIKDPTGVGDAFRAGF
                     ITGLSWGLGLERCAQVGALLATYVIETVGTQEFHLEKSVFIERLTAAYGPTAAQEVKS
                     YLLA"
BASE COUNT          349 a          338 c          255 g          343 t
ORIGIN      
        1 tcaggcatac ctaagcgacc taattcagta tcactggcac aacttgaaag taatagtgaa
       61 ctcgctacta ccgataaacc aaaaccgaga cgtttacgtt gcactttgct atctacctgt
      121 ttcttcactt ggtcaaaacc ctctggaata ctgaacatta cctccagact aacgcaaacc
      181 cctttaaact atgcgtttgc cagggtccct agcgccctag acacccctgc taattgcccg
      241 agaatctggg cttacattca agcagttttg ttagttgcgc cacataagaa actcgactta
      301 tttcctgcac gcctagggac gccagatggt cagtttgcca ttggcagtca atgagtcggc
      361 gctgcccgtc gttcaggtat cgcgccaaaa agaccagggc tgctttggac gcatctgttt
      421 cgagatgaaa catcgattcg ccggcaaata gcccgccgac ttctactccg tataacccac
      481 ctacgatttt gccagccgag ttacgcacct cgacggaatg tgcaaacttc agttcgtgca
      541 actcaagata tgcgtcaata acctgctggg ttatccatcc tgatggccga ttcaaggaac
      601 cgcagtttcg aatcacaaat tcaaaatctt gatcaatagt gaaagtaaat ttcttcagag
      661 atttctttag cgacttagag actttaattt tccccggatc caaaacacca cgtggatttg
      721 gcgaccacca actaataaca agtgggcttt cttttgcatc aagcggcatc gggaagtacc
      781 catttttata ggcccaaatt aaaacttcac tatcccagta actgccatca gcaatgagtt
      841 ttggccaggt acctcctggc gttaggaaaa tgggctgccc cgttgaatcg gtttgaccca
      901 cagcaatcaa ttcaacattt tctggcacag acgccagatc tggaaagtcg tagtttccag
      961 ttttcatgcc aacaagtaag acttaacttc ttgcgccgcc gttggtccat atgcggcagt
     1021 gagtcgttcg ataaaaacac ttttttctaa atgaaactct tgggtaccaa ctgtctcaat
     1081 tacgtaagtg gcaagtaatg caccaacttg agcgcatctt tcgagaccaa gtccccagct
     1141 caatccagtt ataaaaccag ctctaaatgc atccccaaca ccggtcggat ctttgatctg
     1201 tttttcttgg gcaactggaa cccgtaaatc aagtccatct ttggattgaa tgtgaacccc
     1261 atttttacca agagtggtga ctctc
//
LOCUS       DBNHVN010000190         2547 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_36777, whole genome shotgun sequence.
ACCESSION   DBNHVN010000190 DBNHVN010000000
VERSION     DBNHVN010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_36777"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="AC16Y1_03625"
     CDS             <1..1108
                     /locus_tag="AC16Y1_03625"
                     /inference="COORDINATES: protein motif:HMM:NF015734.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIR5831583.1"
                     /translation="NDRLPTARAFHDGVIRAVSAVPQAEALTTALPLTQTAVIPTANQ
                     KPINQPEKISGKAVTSGAIPKSKSAGFFATKKRAFFRLIFALTAVIIGAGAWYMFSGT
                     YQPVPNVAGQTLDQATIAFSDLGLSFEQVEEFSEDIPTGTILRTQPEAGARARVGNPI
                     QVFISKGPERYVIPNDIVGKSTGEVEEILRDLNLVASGTEEIFSAEIEVGKVASTNPA
                     PGASLKRDTPVIIQISKGPEPIDMPNIVGALVTDVAPLLLSLDLTIEVTEEIYDENAP
                     IGAILAINPAAGTKVAKGSVIKATVSLGPPLVSVPNVVGKSVTEATRILEAAGFTVQK
                     VNKLPAAPLQIVYSQNPPANSKVAKGSLITIEIV"
     gene            1133..2056
                     /locus_tag="AC16Y1_03630"
     CDS             1133..2056
                     /locus_tag="AC16Y1_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5831584.1"
                     /translation="MSSVTTTSALAHKKRVEFWAMLLLFAVTFIWGITFVTIKDLLTQ
                     MDPADIMVWRFGIAAAVMFLINPRAIIDLPRKHKIHGIYLGIALGVGFIFQLVGLSIT
                     SVTASAFITGLFVVLTPILAGILLKEPIDKMVWLAVLLTTFGLGLLALKGWSTGLGEL
                     LTLACAFFFALHIIGLDKWSKTDYVYGLTAMQIIVVFVFNLIAAVSTDGISAPPTSEV
                     WTAIIFLAVVATCVGYFAQTWVQSSIGPTRTAIILTMEPVFAGIAGVTLGSDVLTSRM
                     IIGAAFILLGTYIVELGPRHSAEGTHPHLEP"
     gene            complement(2070..>2547)
                     /locus_tag="AC16Y1_03635"
     CDS             complement(2070..>2547)
                     /locus_tag="AC16Y1_03635"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR5831585.1"
                     /translation="VKVGPKTKPEDLLALIEKLNPSNKPGRLMLITRMGADQIRNVLP
                     GLIQAVSKTDHKVIWVCDPMHGNTKEAASGHKTRLFDDVIDEVKGFFEVHKAARTHPG
                     GIHIELTGDDVTECLGGIAGVAESDLPFRYETACDPRLNRVQSLELAFLVSDMLRK"
BASE COUNT          708 a          547 c          538 g          754 t
ORIGIN      
        1 aaatgatcgg cttccaacag ctcgagcatt tcacgatgga gttatccgag ccgtgtcagc
       61 tgtgcctcaa gctgaagctt taacaacagc gttgccgctg acgcaaactg cggtaatacc
      121 taccgcaaat caaaagccaa taaatcaacc ggaaaaaata tccggtaaag cggtaacttc
      181 tggagcaatt cctaaatcta aatcagctgg attttttgca actaaaaaac gtgctttttt
      241 tcgattaatt tttgcactga ctgccgtaat tattggtgcg ggtgcttggt acatgttttc
      301 aggaacctat caaccagtgc caaatgtcgc tggccagaca ctggatcagg ccactattgc
      361 attttcagat ttagggttaa gttttgaaca agttgaggaa ttctcagaag acattcccac
      421 cggcaccata cttcgaacac aacctgaagc cggagcacgg gccagagttg gaaatcctat
      481 tcaggttttc atttcaaaag gtccagagcg ttatgtaatt ccaaacgaca tcgttggtaa
      541 gtcaacagga gaagttgaag aaattctgcg cgatttgaat ttagtcgcct ctggaacgga
      601 agaaatattc tctgctgaaa ttgaagttgg taaagttgca tcgactaatc ctgccccagg
      661 tgcgagttta aagcgggata ccccagttat tattcagatt tcaaaaggtc ctgaaccgat
      721 cgacatgcca aacatagttg gtgcattagt aactgatgtt gctccgttat tactctcttt
      781 agatttaact atcgaggtta ctgaagaaat ttacgatgaa aacgcaccga tcggcgcaat
      841 tttagcgatc aatcccgctg ctggaacaaa agttgctaaa ggttcagtta taaaagcaac
      901 ggtgagccta gggccacccc tggtttccgt accaaacgtt gttggcaaaa gtgtcaccga
      961 agcaacacga attttggaag ctgcaggttt tactgttcaa aaagtaaata aacttccggc
     1021 agcaccactt caaattgtgt attcgcaaaa tcctccagca aacagtaaag ttgccaaggg
     1081 atctttaatc acaattgaaa tcgtttaaaa attagttaag tagttaaaaa taatgtcatc
     1141 tgtcacgacc acttccgctt tagcgcataa aaagcgtgtt gaattttggg cgatgttgct
     1201 gctttttgct gtgacattca tctggggtat cacatttgta actattaaag atctacttac
     1261 ccaaatggat ccggctgaca ttatggtttg gcgctttggg atcgctgcag ccgttatgtt
     1321 tctgatcaat ccgcgtgcga ttattgacct tccgcgaaaa cacaaaatac atggaattta
     1381 tctaggaatt gcattgggag ttggatttat tttccaactt gttgggttat ccataacatc
     1441 ggtgacggct tcagctttta ttactggact ttttgtggtg ttaacgccga ttctggccgg
     1501 aatcttgcta aaggaaccga tcgacaaaat ggtttggtta gcagttttac tgaccacatt
     1561 tggattaggg ttactggctc tcaaaggttg gtctacaggg ctaggtgaat tattaacttt
     1621 ggcttgtgca tttttctttg cacttcacat tattggtcta gataaatggt ccaagactga
     1681 ttacgtttac ggccttactg ccatgcaaat tattgtggtt tttgttttca atctcattgc
     1741 agcagttagc actgatggga ttagtgcacc tccgacatcc gaagtgtgga cagcaattat
     1801 tttcttagcc gtggtggcaa cctgcgtcgg atactttgcg caaacttggg tgcagagttc
     1861 aattggtccg acgcgaaccg caatcattct tactatggaa ccggtttttg ctggtattgc
     1921 tggcgtcaca ctaggttccg acgtgctaac ttcgcgaatg attattggtg cggcatttat
     1981 attgcttggc acttacattg tggaacttgg tcctagacac agtgccgaag gaacacatcc
     2041 acacttagag ccataaatcg aagcagtact tatttgcgca acatatctga aactaagaac
     2101 gccaactcaa gactctgcac tcgattcaat ctcggatcac atgcagtttc atatctaaac
     2161 ggtaaatctg attcagcaac tccggcaata ccgccaagac attcggtcac atcgtcacca
     2221 gttaactcaa tgtgaatacc acccggatga gttcgcgcag ctttgtgaac ttcaaagaag
     2281 cctttaactt catcgatcac gtcatcaaac aaacgagttt tatgtcctga tgctgcctct
     2341 ttggtatttc catgcattgg atcacatacc caaataactt tgtgatcggt tttcgatacc
     2401 gcttgaatta aacctggcaa aacatttcga atctgatcag cacccattct ggtaatcagc
     2461 attaaccgac ctggtttatt actaggattt aatttctcaa ttaaagcgag taaatcttcg
     2521 ggtttagttt tagggccaac ttttact
//
LOCUS       DBNHVN010000191         1466 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1447658, whole genome shotgun sequence.
ACCESSION   DBNHVN010000191 DBNHVN010000000
VERSION     DBNHVN010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1447658"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="AC16Y1_03640"
     CDS             <1..771
                     /locus_tag="AC16Y1_03640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR5831586.1"
                     /translation="SALEDNLALVELNQAVQIPIKDADSLNIPGSGQGRTLTQIAQKL
                     GDESANQWRNYLQHIGELWQDVRAPLVEAQLTQNLIQTVGFKNYLRFRKFSNYLNKNL
                     KNSVLQELAQHYRSQCRVSAKTNLGLLSLNAYVQQIFGVYQPTEGLRELTQALKMRCL
                     ELGVDFKFETPATPVELGGKIIGVELVKNKFTSCDYVVVNDLSDVLLDLNWRSMPSYE
                     TKISSLYVINETSWLGLGPAHAVIGASIVAQLIGSAAD"
     gene            793..>1466
                     /locus_tag="AC16Y1_03645"
     CDS             793..>1466
                     /locus_tag="AC16Y1_03645"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR5831587.1"
                     /translation="MANPLDTNQLRDRVSKTLTDFIADHQKMMIAISSDTNPLVDSLA
                     TLLSGGKRLRPAFAYWGYLAGGGKDRDEVVRACAALEFLQACALIHDDVMDNSDTRRG
                     APAVHKQFESLHASNKWLSDGPRFGTGAAILIGDLALSWADELIFNSGLTTKEVTRAY
                     GVYHEMRTELMAGQYLDLLEQVRGQITVERSATVIRYKSAKYTIEQPLLMGAAIADAT
                     DQVKQTL"
BASE COUNT          432 a          303 c          333 g          398 t
ORIGIN      
        1 tccgcccttg aggacaattt agctttagtg gaacttaatc aggcagttca aattccaatt
       61 aaggatgctg actcgctaaa tatccccgga agtggacaag gaagaaccct aacccaaatt
      121 gcacaaaaat taggtgacga aagtgcaaat caatggcgaa actacttaca gcacataggt
      181 gaactttggc aggatgttcg agcaccttta gtcgaagctc aattaacgca aaacttaatc
      241 caaactgttg gctttaagaa ctacttaagg tttcgtaaat tttctaatta tttaaataaa
      301 aacttaaaga attcagtctt acaagaactt gcgcagcatt acaggtcgca atgtcgggtt
      361 agcgcaaaga caaatcttgg tttacttagt ttgaatgcat acgtgcagca aatctttggt
      421 gtttatcaac ctactgaagg actacgcgaa cttacgcagg ctttaaaaat gcggtgctta
      481 gaacttggcg tcgactttaa gtttgagacc cctgctaccc cagttgagtt aggtggcaaa
      541 atcattggcg tcgaactggt aaaaaataaa ttcacatcct gcgactatgt cgtagttaat
      601 gatctatcag atgtgttgct tgatttaaat tggcgatcaa tgccaagtta cgaaacaaag
      661 atctcgagcc tttatgtgat taatgaaaca tcttggcttg gtctaggtcc agcacatgca
      721 gtaattggcg cttcgattgt cgctcaacta atcgggtctg cagccgatta gttttacctt
      781 tacgctatta gagtggcaaa tccattagat acaaatcagt tgcgagatcg ggtatcaaaa
      841 acgctgactg atttcattgc agaccatcaa aaaatgatga ttgccatatc ttcagataca
      901 aacccactag ttgattcttt ggctaccttg ctttctggtg gaaagcgcct tcggccagca
      961 tttgcttact ggggatacct agccggtggc ggaaaagatc gcgacgaagt agttcgtgct
     1021 tgtgccgcac ttgagttttt gcaggcatgt gcgctaattc acgatgacgt catggataac
     1081 agtgataccc gacgtggtgc gccggcagtc cacaaacaat ttgaaagtct gcacgctagc
     1141 aataagtggc taagtgatgg acctaggttt ggtactggcg cggcaatttt gatcggtgac
     1201 ttagcactct cctgggctga tgagcttatt tttaatagtg ggttaacaac taaggaagtt
     1261 accagagcct acggcgtgta ccacgaaatg cgcacagagc tgatggccgg tcaatattta
     1321 gatttactcg aacaagtccg tggccaaatt acagttgaac gcagcgccac agtaatcaga
     1381 tataaaagtg ctaagtacac aattgagcaa ccgttgttaa tgggtgcggc aattgctgat
     1441 gcaactgacc aagtcaagca gacact
//
LOCUS       DBNHVN010000192         5225 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1521616, whole genome shotgun sequence.
ACCESSION   DBNHVN010000192 DBNHVN010000000
VERSION     DBNHVN010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1521616"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="AC16Y1_03650"
     CDS             <1..478
                     /locus_tag="AC16Y1_03650"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR5831588.1"
                     /translation="VGAIGITTLGSLAGATLLYSLGYFFSDERLEQLVRKYGKYVGIS
                     VKDYTKTITWFERHGSQLVFFGRLIPVIRSLVSIPAGLTSMKLSKFLFFTALGSGIWN
                     TIWITTGYFLGDNWAMAEQYAQILDYVVYSSIAIIGLLFLVKVVKGFINYRNRASN"
     gene            complement(470..1474)
                     /locus_tag="AC16Y1_03655"
     CDS             complement(470..1474)
                     /locus_tag="AC16Y1_03655"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015656235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR5831589.1"
                     /translation="MRSRFLAITVAVVLGLNACSSGDQENAIAIKSALPTPNQTPLIE
                     ENFSDVSLPALMQTEIVGSDLVIENTIGTTSKYTRYAVTYKANDLTISGIMNIPKGDG
                     PFPTLVLGHGYIDTRIYTSGRGLKREQDYLANQGFIVLHTDYRNHAESDDDPENSNNL
                     RLGYTIDVIAAAKALKSSGIPQVDVNRIGYLGRSMGGGIGYNVAIVAPNEFDAIILYA
                     PVSANYVDNFNKWGRGNPEIENPIIERHGSPESNPEFWKGISAENYFDRVDDPIMIHH
                     GTNDESCKIEWSQRANDQLVALGKDVTFHIYEGEQHAFGPQWELSMQRSVEFLNKYLV
                     "
     gene            complement(1528..1878)
                     /locus_tag="AC16Y1_03660"
     CDS             complement(1528..1878)
                     /locus_tag="AC16Y1_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831590.1"
                     /translation="MNKFLTNFSGVTKYLNFFIVPMLISIFLRGELLNLLIPVSIYLG
                     YKRRPEKIWPLWLTAVVCLWATYGLATLTKYLPMDDSGETWWSFGFEAFIFMGLLVAI
                     PMFIGRLISQRINK"
     gene            complement(1875..3221)
                     /locus_tag="AC16Y1_03665"
     CDS             complement(1875..3221)
                     /locus_tag="AC16Y1_03665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen/coproporphyrinogen oxidase"
                     /protein_id="HIR5831591.1"
                     /translation="MYDVVVVGSGLAGLTAAWECRDKELLVLESDNRVGGRIKSEKRG
                     DYWLNTGGHVFSGPGSQTQELADEVGVEIRRIDGHLAGLAMNGALITKGAVELYPLRI
                     PMSFKDRISLVFSGIKVRLAVLYFARISKCKPNEDYWTFQNRIYNYKNDRTFADFVGK
                     TSGDAMAMFAATVTRSGGRVNEVAAGTGIGYFKIVWDKSPSLGNSIWGGASVFPETIA
                     AKLGDRVKLNSPVKEVAIHDNHVEIKYTDLTGDHTVLAKHVVMASPATTTHKVLKNAP
                     MDLMDALAQIKYAPYVAAAFLTNEDKAVWDNSYGIAAPKRSFTIFTNQTSVIRSSEKQ
                     RAKGSSIFVFTATDWAEKLIKEDDQKILETYYKDLNEIFPGFSDRVIEHQITRWHEGQ
                     TFNFPGRAKLQPIFSRDNGRINLAGDFLGTFYTETAITTGKIAGQNILRKLEKETK"
     gene            complement(3266..4654)
                     /locus_tag="AC16Y1_03670"
     CDS             complement(3266..4654)
                     /locus_tag="AC16Y1_03670"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HIR5831592.1"
                     /translation="MPTWVRVAELLAAILFILALKGLSSPKSARRGNLLGALGAALAT
                     AVVFYYGQDFFGGPIKNLNAILIAIAIGAVIGWVAARRVQMTQMPQMVALFNGVGGGA
                     AAVVSVIEFLTAKADEPIAVMAAIVFTVMIGSISFTGSILTFMKLQELMTSRPVVLPA
                     GRYITSLAGIATVAAAIQLVLNQSNLLLFTLLFLSLIMGVLLVLPVGGADVPIVISLL
                     NAFTGLSVAASGYVLGNVLLIVAGTLVGAAGTLLTRLMAQAMGRPLTNTLFGAFTAKA
                     TAAGGTGEDRPVKSASPDDVSIMLAYAQKVIIVPGYGLAVAQAQNTIRELVEILESKG
                     IKVDYAIHPVAGRMPGHMNVLLAEANVPYQQLKEMDEINPEFAETDVALIVGANDVVN
                     PAAKNDPTAPIYGMPILNVDQAQQIVFLKRSMRPGFAGIENEVLFDPKTSLLFGDAKE
                     SLTKVVSALKNL"
     gene            complement(4654..4956)
                     /locus_tag="AC16Y1_03675"
     CDS             complement(4654..4956)
                     /locus_tag="AC16Y1_03675"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha part 2"
                     /protein_id="HIR5831593.1"
                     /translation="MDEIALLTIFILSVFVGIEVVSKVSAVLHTPLMSGANAIHGVVL
                     VGAILVTGKADVALELALGLTAVVLATINMVGGFVVTDRMLEMFKPKKNQGQEANK"
     gene            complement(4959..>5225)
                     /locus_tag="AC16Y1_03680"
     CDS             complement(4959..>5225)
                     /locus_tag="AC16Y1_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831594.1"
                     /translation="KDVSLKVSGGLITIVGCQDLPSQIATHSSKLYAQNIVSLLTLFT
                     KDGNAKPDLADEIIAGSAIVWNGEIKNALTRETLKLKPLAKEGE"
BASE COUNT         1511 a         1141 c         1043 g         1530 t
ORIGIN      
        1 tgttggtgcc attggaatta caacactggg ctctctggca ggagctacct tgctttacag
       61 tcttggctat tttttctctg atgagcgtct tgaacaattg gttcgtaaat atggaaagta
      121 tgtcggaatt tcggtaaagg actatacgaa aactattact tggtttgaac ggcatggttc
      181 gcaacttgtt ttcttcggcc gattaattcc agttatccga agtttggtca gcatcccagc
      241 tggattgacc agcatgaaat tatcgaaatt tttatttttt actgcgctag gtagtggaat
      301 ttggaacact atctggatca ctactgggta tttccttggc gataactggg ccatggctga
      361 gcagtacgca caaatactcg attacgttgt ttatagctca atcgcaataa ttggattact
      421 tttcttagtt aaagttgtca aaggttttat aaattaccgc aaccgcgcct caaactaaat
      481 acttatttaa aaactccact gaacgctgca ttgatagctc ccattgcgga ccaaaagcat
      541 gttgctcgcc ttcgtaaatg tggaaagtaa catctttacc gagtgccact aactgatcgt
      601 ttgctcgctg tgaccattca attttgcaac tttcatcatt agttccgtga tgaatcatga
      661 ttgggtcatc gactcgatca aagtaatttt ctgctgaaat acctttccaa aattcaggat
      721 tggattccgg gctgccatgc cgttcaataa taggattttc gatttcagga tttccgcggc
      781 cccatttgtt gaagttatcg acgtagtttg cacttactgg tgcatataaa atgattgcat
      841 cgaattcatt aggtgcaacg attgcaacgt tgtaaccgat gccgccgccc atggatctac
      901 ctaagtagcc aattcgattc acatcaacct gtggaatacc agagctttta agtgcttttg
      961 cagcagcaat tacatcaatt gtgtaaccga gtctcaggtt atttgagttt tcgggatcgt
     1021 catcgctttc ggcatgattg cggtaatcag tatgtagaac tatgaaacct tgattggcta
     1081 aataatcctg ttcccgctta agtccgcggc ctgatgtgta aatacgcgta tcgatatagc
     1141 catgacctaa aaccaatgta gggaaaggcc cgtctccttt tggaatattc ataattccag
     1201 aaattgttaa gtcgtttgct ttgtaagtaa ctgcgtagcg tgtgtattta ctcgtggtgc
     1261 caatagtatt ttcaatcacc aggtcagaac ccacgatttc tgtctgcatc aaggctggta
     1321 aagaaacgtc actaaagttt tcttcaatta gtggtgtctg atttggcgta ggaagtgcag
     1381 acttaattgc tatggcattt tcctgatctc ccgatgaaca ggcgtttaga cccagaacaa
     1441 cagcgaccgt aatcgccaaa aatctcgaac gcatagtcca aaactatgtc aacaaatgga
     1501 attgcgcatt ttaaagaggt ttataagcta tttattaatt ctttgagaaa ttagtcgacc
     1561 aataaacatc ggaattgcga caagcaaacc catgaaaatg aatgcttcaa agccaaaact
     1621 ccaccaagtt tcgccactat cgtccattgg caaatattta gtaagcgttg ctaggccata
     1681 agtagcccat aaacaaacta cagcagttaa ccacaatggc cagatttttt ctggacgtcg
     1741 tttatatcca agatagatcg acaccggaat taatagattt agtaactcac ctcgcaagaa
     1801 aattgaaatt agcatcggaa caatgaagaa gtttaaatac ttagtaactc ccgaaaagtt
     1861 ggttaaaaat ttattcattt tgtttccttt tctaatttcc gcaagatatt ttgcccagca
     1921 attttgccag tagtaattgc agtttcagta taaaaagttc ccagaaagtc accagctaaa
     1981 ttaattcgac cattatctcg agagaagatt ggttgcaatt ttgctctccc tggaaaatta
     2041 aaagtttgac cctcgtgcca gcgagttatt tgatgttcga taactcggtc tgagaatcca
     2101 ggaaaaatct catttagatc cttgtaataa gtctcaagaa ttttttggtc gtcttctttt
     2161 attaattttt ctgcccagtc ggtagccgta aaaacaaaga ttgaactacc tttggctctt
     2221 tgcttttcac tacttctaat cacgctggtt tggttagtga aaatagtaaa tgaacgttta
     2281 ggtgcagcaa ttccgtagct gttatcccaa accgctttat cctcattggt taaaaatgca
     2341 gcggcgacgt agggagcata tttgatttgg gcaagagcat ccatcaagtc cattggtgca
     2401 ttttttaaaa ctttgtgggt ggttgtagca ggcgatgcca taacgacatg tttagcgaga
     2461 actgtatggt cgccagttaa atctgtgtac ttgatttcga catgattatc atggatcgca
     2521 acttctttga ctggtgaatt caacttaacc cgatcgccta actttgcagc aatcgtttca
     2581 ggaaatacag aggctccgcc ccaaatactg ttcccaagac ttggactttt atcccaaaca
     2641 attttgaagt atccgatgcc agtgccagca gcgacttcgt ttacccggcc accacttcgc
     2701 gttacagttg ccgcaaacat ggccatggca tcaccagaag ttttaccaac aaagtctgca
     2761 aatgtccgat catttttgta gttataaatt cgattttgaa aagtccaata atcttcattt
     2821 ggtttgcact ttgagattcg ggcaaaatac aaaactgcta gtcgaacttt aatgccagaa
     2881 aaaactaaag agattcgatc tttaaatgac atcggaattc gaagtgggta taactcaaca
     2941 gcacctttag ttattagtgc accattcatg gctagaccgg caaggtgtcc atcgatacgc
     3001 ctaatttcca cgccaacttc atcggcgagt tcttgggttt gcgaacctgg accgctaaat
     3061 acgtgaccac cggtatttag ccagtaatcg ccacgctttt cagatttaat tcggccgcca
     3121 acacgattgt cggattcaag aaccaaaagt tctttatctc gacattccca agcagcagta
     3181 agcccagcta atccgctacc gacgacaact acgtcataca taaagacacc ccgtagttaa
     3241 agactacggg gtgttcgtta cttttttaga ggtttttcag agccgaaaca actttagtta
     3301 aagactcttt tgcatcaccg aataaaagtg aagttttagg atcgaataaa acttcatttt
     3361 cgattcctgc aaagcctgga cgcatagagc gtttcaagaa aacaatttgt tgtgcttgat
     3421 caacattcaa aataggcatg ccataaatag gagcagttgg gtcattcttt gctgctggat
     3481 taactacgtc atttgcacca acaatgagcg ctacatctgt ttcagcaaat tctggattta
     3541 tttcatccat ttcctttagt tgttgataag gaacatttgc ttcggcaagt aatacattca
     3601 tgtgccctgg catgcgaccg gctactggat gaattgcgta gtcgacttta atgcctttgc
     3661 tttcaagtat ttctactaat tcacgaatag tgttttgtgc ttgggcgact gccaatccat
     3721 aaccaggaac aataattacc ttttgggcat aagccagcat gatggaaaca tcatccggcg
     3781 aagcagactt aactggtcta tcttcacccg ttccaccggc tgcagttgct tttgctgtga
     3841 acgcgccaaa caaagtatta gtcaacggtc tacccatggc ttgggccatc aaacgggtta
     3901 gcaatgtacc agcggcgcca actaatgttc cagcaacgat aagcaatacg tttcctagta
     3961 cgtagccaga agcagctact gataaaccag tgaaggcatt taatagtgaa atcacgattg
     4021 gtacatctgc gccaccaact ggaagtacaa gtagtacgcc catgatcaac gacaagaaaa
     4081 gcaaagtaaa cagtaataag ttcgactgat taagtactaa ttgaatagca gcagcaactg
     4141 ttgctattcc ggcaagtgaa gttatgtaac gacctgcagg cagtacaact ggacgcgaag
     4201 tcatcaactc ttggagtttc ataaaagtca gaattgagcc agtgaacgag atcgacccaa
     4261 tcatcacggt gaacacaatg gctgccatga ctgcaatggg ttcgtctgcc tttgcagtca
     4321 aaaattcaat tactgaaaca actgcggcag cgcctccacc aacaccatta aatagtgcaa
     4381 ccatctgtgg catctgagtc atttgcactc gccgtgcggc cacccaacca ataacagcac
     4441 caattgcaat tgcgatgaga attgcattta agtttttaat tggtccacca aaaaaatctt
     4501 gaccgtaata gaacacaact gccgttgcta gcgcagctcc gagcgctccc agtaagttac
     4561 cgcgtctggc actttttggt gaagaaagtc ccttaagagc aaggataaat aagatggccg
     4621 cgagtaattc tgcgactcta acccaagtag gcattattta tttgcctcct gtccttggtt
     4681 ctttttgggt ttaaacatct ccaacattcg atcagttact acgaaaccac caaccatatt
     4741 tattgtggct agaacaactg cggttaaacc caaagcaagc tctagcgcaa catctgcttt
     4801 gcctgtgacc aaaattgcgc cgactaaaac aacaccatga attgcattag ccccggacat
     4861 gaggggagtg tgtaataccg ccgatacttt tgaaacaact tcaattccca cgaaaacact
     4921 gagaataaag attgttagta aggctatttc gtccatactt attcgccttc cttagcgagc
     4981 ggctttagtt ttaaagtttc ccgagttagt gcatttttaa tttcaccatt ccagacaatg
     5041 gcacttccag caataatttc atcagccaaa tcaggttttg catttccatc tttggtaaaa
     5101 agtgtcagaa gcgaaactat attctgcgcg tagagcttgc tggagtgagt agcaatttga
     5161 gatggtaaat cttgacatcc gactattgtg attaagccgc ctgagacttt taagctaacg
     5221 tcctt
//
LOCUS       DBNHVN010000193         1448 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_259707, whole genome shotgun sequence.
ACCESSION   DBNHVN010000193 DBNHVN010000000
VERSION     DBNHVN010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1448)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1448)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1448
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_259707"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..>1448
                     /locus_tag="AC16Y1_03685"
     CDS             140..>1448
                     /locus_tag="AC16Y1_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5831595.1"
                     /translation="MAVFGAFISLLPLGYLVVRASEIGLTNLFEVFTRGSALTPLLNS
                     FTLVLLVTFTALIVGSIQAWVIVRTDAKFKPLITVSAIAPLAIPSYIAAYSWLSIFPG
                     FSGIFAAWLVLSLCTSPYVFLAISAALIRNNRTTEEVARTLGYTQWQTFRLITWPEIR
                     TAAYGSGLLAALYVLSDFGAVSLLRFETFTRAIYTAYRAGFDRSTAAALALLLVIVTL
                     FLLAVHNKLPETKLRRSEKTAPAIPIHLGKWTKWVGIGSALWLSAGTLVPVASLTYWN
                     IRGRSETDFAAIGRALINTTGYAIAGGLLVSLIGTAAVVLITRYQSKFSKYIEQSIWL
                     SHALPGIVFALAFVFLSNKFFPSIYQTNWLLLLAYLGLFLPNAISVLKTPFVQIPTAL
                     DEVARSFGNNQLQVIRKVILPAALPGILSAAAFVSLTIIKELPA"
BASE COUNT          373 a          314 c          299 g          462 t
ORIGIN      
        1 cacttgatga aattggtagc cccacaattg atctatctga cttagctact ttggctgaca
       61 cgcaactatt gctaagagaa gcaggattga ttccctgaaa ttaactaaag tacaaagacc
      121 gccagcacta attttggttt tggcggtctt tggtgcattc atttcattgt tgccacttgg
      181 ctacttagta gttcgagcaa gcgaaattgg cttaacaaac ttgttcgaag ttttcaccag
      241 aggatcggcg ctaacaccac tgttgaatag ttttacattg gtactacttg ttacttttac
      301 ggcactaata gttggatcaa ttcaagcctg ggtaattgtg cgaactgatg caaaatttaa
      361 accgctcatt actgtttcag cgatagcacc gcttgcgatc ccgtcctaca ttgcggctta
      421 ttcttggctc tcaatttttc cagggttcag tggaattttt gctgcttggc ttgtactttc
      481 actttgcact agcccctatg tatttcttgc tatctcagcc gcgcttattc gcaataaccg
      541 aacgactgaa gaagtagcta gaacccttgg ctacacacaa tggcaaactt ttcgattaat
      601 aacttggcca gagattcgaa ccgctgctta tgggtctgga ttgttagccg ccctttacgt
      661 attaagtgat tttggtgctg tttcactttt acggtttgaa acttttactc gagcaattta
      721 taccgcttac cgagccggat tcgatcgatc gactgcagcc gcacttgcac ttttactagt
      781 tatagtcact ctgtttttgc tcgccgtgca caataaattg cccgagacta aattacgccg
      841 aagtgaaaag acggctccgg caattccaat tcatttggga aagtggacta aatgggtagg
      901 tattggctca gcactatggt taagcgctgg aactttagtt ccggttgcca gcctgactta
      961 ttggaatatc cgcggcagat ctgaaactga ttttgcggca atcggaagag cgttaataaa
     1021 cacaacaggt tacgcaattg ccggtggctt gctagtttcg ctaattggca ctgccgcagt
     1081 tgttctgatt actcggtatc agtcaaaatt ttcaaaatac atcgagcaat ctatttggct
     1141 aagtcatgcc ctacccggaa tagttttcgc gttagcgttt gtatttctaa gtaataaatt
     1201 cttcccgtcg atttatcaaa caaattggtt attattgctg gcttacttag ggctattttt
     1261 accaaatgca atttcagtgt taaaaactcc ttttgtccaa attccaactg cgctagatga
     1321 agttgctaga agttttggaa ataaccaact tcaggtgatt agaaaagtta ttttgcctgc
     1381 agcactgccc ggtattttaa gcgccgctgc atttgtttca ctaacaatta ttaaagagct
     1441 gccagcaa
//
LOCUS       DBNHVN010000194         2435 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_259731, whole genome shotgun sequence.
ACCESSION   DBNHVN010000194 DBNHVN010000000
VERSION     DBNHVN010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_259731"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2159)
                     /gene="secA"
                     /locus_tag="AC16Y1_03690"
     CDS             complement(<1..2159)
                     /gene="secA"
                     /locus_tag="AC16Y1_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009188930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR5831596.1"
                     /translation="MSVLDKILRAGEGKILRRLESISKQVNALEPHFTEMSDEELKSM
                     TPEFRSRLAEGETLDDLMPEAFAVVREAAKRTLGQRHYDVQIMGGAALHLGNIAEMRT
                     GEGKTLVSTLPAYLNALAGNGVHIVTVNDYLAARDAQWMGRVHRFLGLSVGTILANME
                     PPLRRAAYEMDITYGTNNEFGFDYLRDNMAHVPTDRVQRGHNFAIVDEVDSILIDEAR
                     TPLIISGPADQATKWYAEFARLVPRLVRDTDYEVDEKKRTVGILDSGVEKVENWLGIE
                     NLYESVNTPLVGYLNNALKALNLFKRDKDYVVMNGEVLIVDEHTGRMLAGRRYNEGMH
                     QAIEAKEGVPIQNENQTLATITLQNFFRLYDKLAGMTGTAMTEAAEFNQIYKLGVVPI
                     PTNKSAQRIDAVDLVYRTEDAKFKAVIADIVERHRNGQPILVGTASVEKSELLAQYLT
                     RAGIAHNVLNAKQHEREASIIAEAGRKGAVTVATNMAGRGTDIMLGGNPEFRAQKALA
                     DRGLDPVENPTEYEAAWPAAIKAAQEAVATEHEEVISCGGLYVLGTERHESRRIDNQL
                     RGRSGRQGDPGETRFYLSLQDDLMRLFKGEIVNNFLTRFNVPDDVPIEAKMVTNAIKS
                     AQTQVEAQNFEIRKNVLKYDEVMNRQRLVIYSERNRILDGEDVAEHIQNFMHDTIDAY
                     VQAATAEGYPEDWDYDTLWTALKTLYPITLTLEDVIK"
BASE COUNT          618 a          584 c          518 g          715 t
ORIGIN      
        1 tctttaatta catcttctag cgtcagggtg attgggtaca acgtctttag cgcagtccaa
       61 agtgtgtcgt aatcccaatc ttctgggtaa ccttcagcgg tagcagcttg aacatatgca
      121 tcgatcgtgt cgtgcatgaa gttctgaatg tgctcggcaa catcttcacc atcaagtatt
      181 cgatttcgct cactatatat aactaagcgc tgacggttca ttacttcgtc atatttcaaa
      241 acgtttttac gaatttcgaa gttctgtgct tctacttgag tctgtgcact cttaatcgca
      301 ttagttacca tctttgcttc gatcggtaca tcgtcaggca cgttgaatcg agttaagaag
      361 ttattaacta tctcgccctt gaataagcgc attaagtcgt cttgcagtga aaggtagaac
      421 cgagtttcac ctggatcacc ttgacgacca gaacgaccgc gtaactgatt atcaattcgt
      481 cgagattcgt gacgttcggt acctaagaca taaagtccgc cgcaagaaat aacttcttca
      541 tgttcagttg ccacagcttc ttgtgcagct ttaattgctg ctggccaagc agcttcatat
      601 tcagttggat tttcaactgg atcaagtcct cgatcagcca atgctttttg ggcgcggaat
      661 tctggattac caccaagcat gatgtcagta ccacgaccag ccatgttggt tgcaacagtc
      721 actgcacctt tacgacctgc ttcagcaata atcgaggctt cacgttcgtg ttgttttgca
      781 tttaaaacat tgtgtgcaat tccagcgcgg gttaagtact gcgcaagtaa ctcgcttttc
      841 tcaactgatg cggtaccaac aagtattggc tgaccgttac ggtggcgctc aacaatgtca
      901 gcaataacag ccttgaactt ggcatcttcg gttcggtaaa ccaagtcaac cgcatcaatt
      961 ctttgagctg acttattggt cgggattggc accacaccaa gtttgtaaat ctgattgaat
     1021 tcggcagctt cggtcatcgc cgtaccagtc ataccggcta acttgtcgta caaccggaag
     1081 aagttttgca aagtaatggt ggccagagtt tgattctcat tttgaatcgg cacaccttct
     1141 ttagcttcaa tggcttggtg cataccttcg ttataacgac gaccggccaa catgcgaccg
     1201 gtgtgctcgt caacgattaa aacttcaccg ttcataacga cgtaatcttt gtcgcgctta
     1261 aacagattta aggccttaag tgcgttattt aagtaaccaa ctaaaggtgt attaactgat
     1321 tcataaaggt tttcgatacc aagccagttt tcaacttttt cgacaccaga atctaaaatg
     1381 ccaacagtgc gtttcttttc atcaacttcg taatctgtat cgcggactaa acgtggaact
     1441 agtcgagcaa attctgcgta ccacttagtt gcctgatcag ctggcccact aataattaat
     1501 ggcgttcttg cttcatcgat caagatcgag tcaacttcgt caacaattgc aaagttgtgt
     1561 ccgcgctgta cgcgatcagt aggtacatgt gccatgttgt cgcgcaggta atcaaaacca
     1621 aactcgttat ttgtgccgta agtaatgtcc atttcatagg cggctctacg aagcggtggt
     1681 tccatgttgg ccaaaattgt gccaacgctt aaacctaaaa atctgtgtac tcggcccatc
     1741 cattgtgcgt cgcgggccgc aaggtagtcg ttcacggtaa caatgtgcac gccattacca
     1801 gcaagtgcat ttaaataagc tggcaaagtt gaaactaaag ttttaccttc accggttcgc
     1861 atttcagcga tgttgccaag atgtagtgca gcgccaccca tgatctgcac gtcataatgc
     1921 cgttgaccaa gtgtgcgctt tgcagcttcg cgaactaccg caaaagcctc gggcattaag
     1981 tcatcaagag tttcgccctc agccagtcga ctgcgaaact ctggggtcat cgatttcagt
     2041 tcttcgtctg acatctcggt gaaatgcggc tctaaggcat tgacttgctt ggaaatagat
     2101 tcaaggcggc gcaaaatttt gccttcgcca gcgcgaagta ttttgtctag aacgctcaac
     2161 tcttaatcct caaatgtgcc gaactactga atagccctac ttttaaccct tctaatcgta
     2221 ggtcacgctt caattggcac agactgccca ttgagttgat tgctgtgagc gaaatcgcta
     2281 aaaatctggg gtttttcaag tgtcgtttcc acgagtgact gtatttaccg tgttattttt
     2341 aaaaaacacc tttttccccg aaagtaaggc aatgaagctt ccccgcttac attttgcgct
     2401 agtcgctgca cttggtctaa gcctgctttc aatga
//
LOCUS       DBNHVN010000195         5998 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_2042057, whole genome shotgun sequence.
ACCESSION   DBNHVN010000195 DBNHVN010000000
VERSION     DBNHVN010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5998)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5998)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5998
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_2042057"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="AC16Y1_03695"
     CDS             complement(<1..591)
                     /locus_tag="AC16Y1_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5831597.1"
                     /translation="MSAKSEFDRLSGTYFVAGKHKKSSGAAAFQVTNPATGENLGFVA
                     ECTEQEINEVFDAAKGAQKEWWAQSALHRAEVLHEVARKIKANRLELAELLTREMGKT
                     FKESADEVDWSVTALDYYAEIGRHEGGHVIGPATAGQFHFTIKQPMGTVGIILPFNFP
                     YVLLCWEAAAALVAGNAVVVKPSEHTSLCTLKFMEFF"
     gene            791..3241
                     /locus_tag="AC16Y1_03700"
     CDS             791..3241
                     /locus_tag="AC16Y1_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcvT family protein"
                     /protein_id="HIR5831598.1"
                     /translation="MSSPAIPSEARVVIIGGGVIGTATAYQLAKRGWTDIVLLEKNTL
                     TSGTTWHAAGLITQARPTHGMRSIVTRGLKTFKSLEEDTGFSTGYVETGTIHLAMTEA
                     RLEELKHQELASISSQIEVKLLNRDQTLELHPLLNPDGVYGSLFYPYEGRGSATDTTI
                     SLARGATQRGAQVVEQVTVTGVLTDKNKVVGVTTTAGDIKAQYVVNATGMWGREFGEL
                     AGVQIPLQALAHYYIVTEGIENLPRNLPTIKSADDCSYVKDEAGALLVGFFEPDSYAW
                     ASKGIPENDGFIRLPEDWDHLGPYYERMIERMPILADAGVRLHFCGPESFTPDGIYHL
                     GPAPKLENYFVAAGFNSTGFLSGPGAGATLADWIIDGYSPIDMPETDLTRVQPHETNR
                     RFLEERVVETLDMSYGVHWPFEQRKSARPLRVSPLYDSTTAAGAVYGQLLGWERANWY
                     AQTEDEKEYRYTWDRPNWFNANKREHEAVRNNVGIFDTSSFGKLLVQGKDALKVLQRL
                     SIRNVDVAVGQVVYTQWLNIFAGIEADVTVTRVEEDQFLVLSGPGTHLRDLNTLKREV
                     KDGEFCTVADVTGTLAMISVMGPKSREMLQGLTDCDLANDKFKFGQSKEIDLGLGYVR
                     ATRLTYVGELGFELLIPTELSRHIYATLTQAGQQFEMLPAGYYALGSLRLEKGYRSWG
                     HDISSNDDPVSAGLDFGVDWDKDGGFIGLSALTRAKQQGSNRRLMQFVLQAPDEFLLH
                     DEPIFRDGKLVGRCGSTGYGHTLGGPVTLAWIEAAGIESDEWFTAGKYEIQTVNGKFA
                     STASLKPQYDPTSSRIKS"
     gene            complement(3238..4725)
                     /locus_tag="AC16Y1_03705"
     CDS             complement(3238..4725)
                     /locus_tag="AC16Y1_03705"
                     /inference="COORDINATES: protein motif:HMM:NF019520.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006144 - purine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HIR5831599.1"
                     /translation="MSITIAQIVANPSLRTVFIAGQSGGERTVDWAHNTDVSEPWHWL
                     SAGELLLTLGRNFPATAEEQISFIENLANAGIAGLDVAAGWYAPELTPEAAKYADEIS
                     FPIIQTAYETPFVLISRYVAEVTSKTNQLSKILQMYEFYRQSSLRRESADAQLEALEK
                     HVSMDLTILDTKSLRSILPFKRKLPENLLDQISSALKKSPLPAVFRLKNAEDNYLLVS
                     IGSQDQILVVNTKTENVDLVALQHTASVVAFIAERYSLEASTRATTGRRLLTQLLENL
                     VDRELAKERLEQFGLDKGPWRVACPQGNLPIDLEYIHTVFNRKNIPAIATVGSKGLLV
                     LTNADFAWTEEFLNLLVGSISSVGLSSQINSLGRLADGAREANWARESTLAQGIRVGT
                     YGETDSLFLPRTVAAAEIAVNSILGPLLKHDQEHSGELIKSLETFFAANMSWQKASQE
                     LGIHRQTLNYRMQKVFELTNHNVNNIQELTELHLAIKAKNMLDRI"
     gene            4910..5869
                     /locus_tag="AC16Y1_03710"
     CDS             4910..5869
                     /locus_tag="AC16Y1_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831600.1"
                     /translation="MFKRIVTAAAAGVLLAACGTGTQQENIESSDLENTSGHLGWTYE
                     DLKPLNIPGVSPEVIFEDGTIYLYSTDMGIKLYKSSDGLTFELEANAKMPMAADPTII
                     KTSDGKWNMYVTEVMMNGPKPEPGQAPQPMDPKKTQKIVKVSTSDSLENFGPGVETGI
                     AQAKPGAAWGVPDTTVDPSGKIRMFWVDMNEGDKWESIKSATSTDGITFSVDEGIRFT
                     DAYVDPYVLKAQEGDWVMLLSTTPARERLPQKFYLAWSTDGLNWKVDNEPLIDNPDKN
                     YLDPTAYEISPGVWRVYVSTSDKANAIGGPYSLETFILKAPTN"
BASE COUNT         1741 a         1229 c         1325 g         1703 t
ORIGIN      
        1 aaaaaattcc atgaacttta gagtgcacag actggtgtgt tcagatggct taactacgac
       61 agcatttccc gcaacaagcg cggcggcagc ctcccaacaa agtaaaacat aagggaagtt
      121 aaagggaaga ataattccaa cagttcccat cggttgctta attgtgaagt ggaattgacc
      181 agcagttgcc ggcccaatta catgaccgcc ttcatgacga ccaatttcgg catagtaatc
      241 aagcgcagtt actgaccaat caacttcgtc cgcactttct ttaaaagttt tacccatttc
      301 tcgagtgaga agttcagcca attcaagccg gtttgctttt atttttcgag caacttcgtg
      361 gagcacttca gcccgatgta aagctgactg cgcccaccat tctttttgcg caccttttgc
      421 tgcatcaaac acttcattta tttcttgttc ggtgcattca gccacaaagc caaggttttc
      481 cccagttgct gggttagtca cttgaaatgc tgcagctcca ctactcttct tatgctttcc
      541 agcaacaaag tatgttccac tgagtcgatc gaattctgat ttggctgaca tttgaggcag
      601 tcccttcagg ggttgagcaa gttatttttt gagttattaa tgctcagact gccagtttcg
      661 aattcaaagg caattgtgca aatgcttgaa ttcctgcaga cgaattagtc attcgtaaca
      721 ttgctcaaat gaccatcacc cacaacgatc agttagagat cttgactaaa aaacgtttaa
      781 aaggtgaatt atgagcagtc cagccatccc gagtgaagcc agagttgtaa tcattggggg
      841 aggcgtcatc ggcactgcta ctgcctacca actagcgaaa cgtggttgga ccgacatagt
      901 tttactcgag aaaaataccc tcacttcagg aacaacctgg catgcagctg gcttaattac
      961 ccaagcaagg ccaacacacg gaatgcgatc aatagtcact cgtgggttga aaacctttaa
     1021 gtcattagaa gaagacaccg gattttcaac cggttatgtt gaaactggca cgattcactt
     1081 agcaatgacc gaagctcgcc ttgaagaact taagcaccaa gagttggcct caatttcaag
     1141 tcagattgaa gttaaattac tcaatcgtga tcaaactctg gaattacatc cattgttaaa
     1201 tccggatgga gtttatggaa gcttgttcta tccatatgaa ggcagaggta gtgcaaccga
     1261 caccacaatt tcgctcgctc gcggtgcaac ccaacgcggg gctcaagttg tcgaacaagt
     1321 cacggtaact ggagttttaa cagacaagaa caaagttgtt ggagtgacta caactgctgg
     1381 agatataaag gctcaatatg tagtaaacgc aacaggtatg tggggtcgcg agtttggtga
     1441 actggctgga gtgcaaattc cactacaagc tttagcgcat tactacatcg ttacagaagg
     1501 tattgaaaat cttccacgaa atttgccaac tataaaaagt gctgatgatt gttcttatgt
     1561 taaagatgaa gcaggcgcgt tactagttgg attttttgaa ccggatagtt atgcctgggc
     1621 ttcaaaaggt attccagaga atgatggttt cattaggttg ccagaagact gggatcactt
     1681 gggaccttat tacgagcgca tgattgagcg gatgccaatc ttggcagatg ccggagtacg
     1741 tctacatttc tgtggtccgg aaagttttac accagatggt atttatcact taggtccagc
     1801 accaaaatta gaaaactatt ttgttgcagc aggctttaac tcaaccggat tcctgtctgg
     1861 tccaggcgct ggagccacat tggctgactg gatcattgat gggtattcac ctatagatat
     1921 gccagaaacg gatttaacta gagttcaacc acacgaaacc aatcggcgat ttcttgagga
     1981 aagagtcgtt gagactttag atatgtctta tggggttcac tggccctttg aacaacgtaa
     2041 aagtgcaaga cctcttcgag ttagtccact ttatgattca actaccgcag ctggcgcggt
     2101 ttatggacaa ttattgggct gggaacgcgc caattggtat gcccaaaccg aagatgaaaa
     2161 agagtacaga tacacctggg atcgacctaa ctggtttaac gcaaacaaac gcgaacatga
     2221 agcagtcaga aacaatgtcg gtatttttga tacatcttca tttggcaaac ttttagtaca
     2281 aggaaaagat gcacttaaag ttttgcagcg actctcaatt agaaatgttg atgttgctgt
     2341 cgggcaggtt gtgtacacac aatggctaaa tatttttgcc ggcattgaag ctgatgttac
     2401 tgtcaccagg gttgaagaag atcaattctt agttttgtct ggaccgggaa cccacttacg
     2461 tgatttaaac accttaaaac gtgaagttaa agacggtgaa ttctgcacag ttgccgacgt
     2521 gactggcact ttagcaatga tttcggtgat gggtccaaag tcacgtgaga tgcttcaagg
     2581 tttgactgat tgtgacttgg caaatgacaa attcaaattt ggtcagtcta aagaaattga
     2641 tttaggactt ggttacgttc gagctactcg attaacttat gttggagaat taggatttga
     2701 attattaatc ccaaccgaac tttctcgaca catttatgca actttgactc aagctgggca
     2761 acaatttgaa atgttgccgg ctggttatta cgcgcttggt tcacttcgat tagaaaaggg
     2821 ttaccgaagc tgggggcatg acatttccag taacgatgac ccagtgagcg caggcttaga
     2881 tttcggcgtt gactgggaca aagatggtgg cttcattgga ctgagcgcat taactcgagc
     2941 caaacaacaa ggaagtaatc gcaggctcat gcaattcgta ttgcaagctc cagatgaatt
     3001 tttactgcac gatgaaccga tttttcgaga tggaaaatta gtcggtaggt gtggttcaac
     3061 gggttatgga cacaccctgg gcggaccagt aactcttgct tggattgaag cagccgggat
     3121 tgaatcggac gaatggttca ctgcaggaaa atatgaaatc caaaccgtta atgggaagtt
     3181 tgcgtcaact gcatctttaa agccacagta tgacccaact tcaagtcgga ttaagagcta
     3241 aattctatcg agcatgtttt tagctttaat agctagatgt aactcggtta attcttgaat
     3301 gttattaacg ttgtggttag ttaattcaaa tactttttgc attcggtaat tgagagtttg
     3361 gcgatgtatg ccaagttctt gacttgcttt ttgccatgac atatttgccg caaaaaaagt
     3421 ttctagtgat tttattaatt cgccagaatg ttcttgatcg tgtttcaaaa gtggtccaag
     3481 aattgaatta accgctattt cagcagcagc cacagtccga ggcaaaaata gtgaatcagt
     3541 ttcaccataa gttccgacgc gaattccttg agctaatgtc gattcgcgcg cccaatttgc
     3601 ttcacgggct ccatctgcta gtcgacccaa actgtttatt tgtgaactta agccaacact
     3661 actaatcgag ccaactagca agtttaagaa ttcttcagtc caagcaaaat cagcattggt
     3721 taaaactagt aagcccttag agccaacagt cgcgattgca ggaatatttt ttcgattaaa
     3781 tacggtatga atgtattcca aatcaattgg caaatttcct tgggggcaag ctactctcca
     3841 aggaccttta tccagtccaa attgctcaag ccgctctttg gctaactcac gatcaactaa
     3901 attttctaat agttgagtca gaagccgccg ccctgttgtc gccctcgtgc ttgcttctaa
     3961 tgagtagcgc tcagcgataa atgcaacaac agaagcagta tgttgaagcg ctactagatc
     4021 aacattttca gtttttgtat taaccaccag aatttggtct tggctaccaa tcgaaactag
     4081 caagtagtta tcttctgcat tttttaaccg aaaaactgca ggaagaggtg attttttcaa
     4141 tgcggaactt atttgatcaa gtaaattttc tggaagtttt cgtttaaaag gaagaatcga
     4201 ccgaagtgat ttggtgtcta aaattgttaa atccatagaa acatgttttt ctagggcctc
     4261 aagttgtgca tctgctgatt cccgccggag tgacgattgt cgataaaatt catacatctg
     4321 caatattttt gatagctgat ttgtttttga tgttacttct gcaacatatc gactaattaa
     4381 aacaaaaggt gtttcgtagg ctgtctgaat aattggaaaa cttatttcat ctgcatattt
     4441 ggccgcttct ggagtcaatt caggtgcgta ccaaccagcc gctacatcta gtcccgcgat
     4501 accagcatta gctaaatttt caatgaagga tatttgctct tccgccgttg ctggaaaatt
     4561 tcgcccaagt gtgagaagca actcgccggc actgagccaa tgccaaggtt cactaacgtc
     4621 agtattgtgg gcccagtcga cagttcgttc tccaccgctc tgaccagcga tgaatactgt
     4681 gcgcagactt ggattagcaa caatctgcgc gatagttata gacaccatta actccgaatt
     4741 taaaaatctt tagttagtga cactagtaag cgtaattcga gtaatcccca attactttga
     4801 cgttttagaa atagacatca attttgattt tatggggata gctagaaaaa tcctcgaaat
     4861 ttaattgaaa ttgcagcaat ttatcgaggt tttgagcaag gatttactta tgtttaagag
     4921 aattgttact gcggccgcag caggtgtctt gttagccgcc tgcggaacag gcacccaaca
     4981 agagaacatt gaatcttctg atctagaaaa tacttctgga caccttggtt ggacttatga
     5041 agacttaaag ccgcttaata ttcctggtgt ttctcctgaa gtgattttcg aggatggaac
     5101 tatttacctg tattcaacag atatgggtat caagctttat aaatcctctg atggtttaac
     5161 ttttgaacta gaagcaaatg ccaaaatgcc aatggctgcg gatccaacaa ttattaaaac
     5221 ttcagatggt aagtggaata tgtatgtcac cgaagtaatg atgaatgggc caaaacctga
     5281 gccaggccaa gccccgcaac ccatggatcc aaagaaaact caaaaaattg taaaagtatc
     5341 gacaagtgat tcactcgaaa attttggacc tggagttgaa actggcattg ctcaagccaa
     5401 gccaggcgca gcctggggcg ttcctgatac caccgttgac ccatctggaa aaattcgcat
     5461 gttttgggtt gacatgaatg agggtgacaa atgggagtca attaaaagtg caacaagcac
     5521 tgatgggatc acattctcgg tggatgaagg cattcgattt actgatgctt atgtcgatcc
     5581 ttatgttctt aaagcgcaag aaggtgattg ggttatgttg ctatcaacaa cgccggcacg
     5641 agagcgacta ccgcaaaaat tttatttagc ttggtcaact gatggtttga actggaaagt
     5701 agataatgaa ccgctaatcg ataacccaga taagaattat ctcgatccaa ctgcctatga
     5761 aatttcacct ggtgtttggc gagtgtatgt atccacaagc gataaagcca atgcgattgg
     5821 tggcccttac tcattagaaa catttattct gaaggctcca acaaattagt gccactattg
     5881 agttagaaaa cccttagaat ttcgcggttt tgggcaaatt tcggtacgct atctgggcgc
     5941 aagaatattt ggctggcccc cttagctcag taggtagagc gtttccatgg taaggaaa
//
LOCUS       DBNHVN010000196         3884 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1968040, whole genome shotgun sequence.
ACCESSION   DBNHVN010000196 DBNHVN010000000
VERSION     DBNHVN010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1968040"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..587
                     /locus_tag="AC16Y1_03715"
     CDS             72..587
                     /locus_tag="AC16Y1_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017545285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HIR5831601.1"
                     /translation="MDSKFPGLAFSDVLSSNQQFVLDFKSQHLTGRAAKGLAIITCMD
                     SRISPLAMVGMQAGDVKILRNAGARVTDDVLRTLVLATFLLGVNRVLVVPHTDCRMAN
                     SDEIELHKTILDDFGVDTRSVEFRTVKDQLAALEQDVTRIRTYPLLAKGVTVGGAIYD
                     VNTGQLMPQDI"
     gene            601..798
                     /locus_tag="AC16Y1_03720"
     CDS             601..798
                     /locus_tag="AC16Y1_03720"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HIR5831602.1"
                     /translation="MPFISVTMLEGRTADQKLALIRALTDAIVETAGAKRENVHVAIN
                     DVSLDEWGTGGMSMREIKAQS"
     gene            complement(795..2975)
                     /locus_tag="AC16Y1_03725"
     CDS             complement(795..2975)
                     /locus_tag="AC16Y1_03725"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018547356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit B"
                     /protein_id="HIR5831603.1"
                     /translation="MPVKSSKKSSSKAKPQKAKPVKSAAAKPAIKQSAVKRSTAKQST
                     DYNAADLSVLEGLDAVRKRPGMYIGSTDGRGLMHCLWEIIDNSVDEALAGHCKNIEVI
                     LHGDTIAEVHDDGRGIPVDVEPKTKLTGVEVVMTKLHAGGKFGGGSYTASGGLHGVGA
                     SVVNALSSRLDVEVDREGATHAMSFQRGNPGLFSGKDEEAKFTKQGGLKKVAKLAKKS
                     TTGTRIKFWADQQIFTKDAAFDLKAIHDRARQTSFLVPGLKIRVVDNRIKQTDEFVHV
                     GGISEYAEFLSTGELVTPIIRLKGSGHFHETIPVLDEHGHMNPREVERDMGVDIALMW
                     NSTYETTLQSYVNIISTPKGGTHVQGFDRALVKVINDQLKANRLLKANEDAVIKDDVQ
                     EGLVAVVTVRVSEPQFEGQTKEILGTPAATKIVNAVVTKELTNWFTNPPRGAKPATKA
                     ALEKIANASRARIAARTHRDVQRRKTALESSTLPAKLKDCRSEDTERTELFIVEGDSA
                     LGTAKTARNSEYQALLPIRGKILNVQKASLADMLKNAECASILQVIGAGSGRSFDLDS
                     VRYNKIIILADADVDGAHIRTLLLTLFHRYLTPMLEAGRVYAAVPPLHRIEVIGGGGK
                     KSNEIIYTYSDAEMKATLTDLTKQGRKWKEPIQRYKGLGEMDAVQLRETTMDPEKRTL
                     RRITTGDAKSADDVFDLLMGNDVGPRKDFIIEGAAKLDRERIDA"
     gene            3167..3367
                     /locus_tag="AC16Y1_03730"
     CDS             3167..3367
                     /locus_tag="AC16Y1_03730"
                     /inference="COORDINATES: protein motif:HMM:NF048765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7455 domain-containing protein"
                     /protein_id="HIR5831604.1"
                     /translation="MSATLTRTPLTSSDRCDRCAAQAHVRVYLENGSALQFCGHHFRQ
                     HEVRLRSVAVDIQDELAKLADR"
     gene            complement(3419..>3884)
                     /locus_tag="AC16Y1_03735"
     CDS             complement(3419..>3884)
                     /locus_tag="AC16Y1_03735"
                     /inference="COORDINATES: protein motif:HMM:NF037564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3048 C-terminal domain-containing protein"
                     /protein_id="HIR5831605.1"
                     /translation="VTDSSASPAPTAAQNAAAAAGESVAKIGLEFSKGNKPNWTWDNT
                     SGTWLRDDGSKPHMAISGNQLSAKNLVVLRITTVDAGYKDPAGGYVPRTVLEGTGPGF
                     AVLGNKKVDVTWSKPDVKSPITLTDGSGNVVGLLPGNTWVELIPEEGNVTFQ"
BASE COUNT         1001 a          908 c          854 g         1121 t
ORIGIN      
        1 taaattggct aaagcagtct ctgccgttaa ttcaaagcct acttttaatt aacccgtgac
       61 agaataagca cgtggattcc aaatttcctg ggttagcttt tagtgatgtg ctttccagta
      121 atcagcaatt tgttttagat ttcaaaagtc aacatttaac tggtcgagcc gctaaaggtc
      181 ttgccatcat cacctgcatg gactcaagaa tcagtccgtt ggctatggtt ggaatgcaag
      241 ctggtgacgt aaaaatactt cgtaacgctg gtgctcgggt aaccgatgac gtactgcgaa
      301 ctttggtcct ggctacattc ctacttggcg taaatcgagt actagttgtg ccacatacag
      361 attgccgaat ggcaaattca gatgaaatcg aactgcacaa aactattctc gatgattttg
      421 gcgttgacac tcgcagcgtt gaatttagga cagttaaaga tcagctagcg gcgttggaac
      481 aagatgtgac ccgaattcgt acttacccac tgcttgccaa gggagtaaca gttggtggcg
      541 caatttacga cgtaaacact ggccaactga tgcctcaaga catttaatca aaggagaatc
      601 atgccattta tttcagtgac tatgctcgag ggccgcactg cagatcaaaa acttgctttg
      661 atccgtgcgt taacagatgc aattgtggaa accgcaggag ctaaacgcga aaacgtgcac
      721 gttgcaatca atgatgtgtc acttgatgaa tggggcaccg gcgggatgtc gatgcgcgaa
      781 atcaaagcgc aaagttaagc gtcaattcgt tctcgatcta atttggccgc accctcaata
      841 ataaagtctt tgcgtgggcc aacatcgtta cccattaaga gatcaaaaac atcgtcggca
      901 ctctttgcat cgccggtcgt aattctacgt aaagtgcgtt tctctggatc catagtggtc
      961 tcacgcaact gaacagcgtc catttcacca aggcccttat aacgttgaat tggctctttc
     1021 catttacgac cttgcttagt tagatcggta agagtggctt tcatttcggc gtcgctatag
     1081 gtgtaaataa tttcatttga cttcttacca ccgccaccga tcacttcgat tcgatgaagc
     1141 ggtggaacag ctgcataaac gcggcccgct tccagcattg gcgtcaaata ccgatgaaat
     1201 aaggtcagca ataaagtgcg aatatgtgcg ccatcaacgt ccgcatcggc caaaataatg
     1261 atcttgttat accgaacgga atctaaatcg aaagatcttc ctgaacctgc accaataacc
     1321 tgcaaaattg aagcgcactc ggcattcttg agcatgtcgg ctaatgacgc cttttgaaca
     1381 ttgagaatct ttccgcggat tggcaacaat gcctgatact cactattgcg cgcggtttta
     1441 gcggttccta gtgcgctatc tccttcgaca ataaacaatt cagtacgttc agtgtcttcg
     1501 cttcgacaat cttttaattt cgctggcaaa gtcgatgact ctagtgcggt tttacggcgt
     1561 tggacatctc gatgtgttct ggcagcaatc cgagcacgcg aagcattagc aattttctct
     1621 agtgcggctt tcgtggctgg cttagcaccg cgcggcgggt tggtaaacca attcgtcaat
     1681 tcctttgtca ctaccgcatt gacaattttt gtcgctgctg gcgtaccgag tatctcttta
     1741 gtttgacctt cgaattgtgg ttcagaaact cgaacggtaa caacagcaac taaaccttct
     1801 tgaacgtcat cttttataac tgcatcttcg ttagctttta gtaatctatt tgcttttaac
     1861 tgatcattaa ttacttttac caacgcccga tcaaagcctt gtacatgcgt tccacctttt
     1921 ggcgtggaaa taatgttcac ataagactgc agagtagttt catatgttga attccacatt
     1981 aaagcgatgt ctacgcccat atctcgctca acttcacgag ggttcatgtg cccatgttca
     2041 tcgagcactg gaatagtttc gtggaagtgc ccactacctt taagtcgaat aattggcgta
     2101 actaattcac cagttgatag aaattcggcg tactctgaaa ttccgccaac atgaacaaac
     2161 tcatcggttt gtttaattcg gttatcaact accctgatct ttaggccagg aactaagaaa
     2221 gaagtttgcc ttgcccgatc gtgaatcgct ttcaagtcga aggcagcatc tttagtgaaa
     2281 atctgttggt ctgcccaaaa tttaattcga gtaccagttg tggatttctt tgctaatttg
     2341 gcaacctttt tcaggccgcc ctgtttggtg aacttggctt cttcatcttt gccactaaat
     2401 aaccctggat tacctcgctg aaatgacatg gcatgagttg ccccctcgcg gtctacttca
     2461 acgtctaatc tgctggataa agcgtttact actgatgcac cgacgccatg taagccaccg
     2521 gaagctgtgt aagaaccgcc accgaacttt ccacctgcgt gcagtttcgt catcacaact
     2581 tcaacaccag ttagcttggt ttttggttcc acgtctactg gaattccgcg gccatcatcg
     2641 tgcacctcag cgattgtgtc gccatgcaag ataacttcaa tgtttttaca gtgaccagct
     2701 agcgcttcat cgactgaatt atcaataatt tcccaaagac agtgcattaa gccacggcca
     2761 tcagttgatc caatgtacat acccggacgc ttacgaactg catccaagcc ttcgagcacg
     2821 gaaagatcgg cagcgttgta atcggtactt tgtttagcag tacttcgttt tacagcagac
     2881 tgtttgatag cgggtttggc ggcagcggac ttaactggtt ttgctttttg aggcttagct
     2941 ttgctgcttg atttcttgga agatttaacc ggcataacct tataagacta tgaaatattc
     3001 atagattttc ttggattacg cgccgtgctt gtgaatttga gaaaacttcg ccaatagaga
     3061 acttggctat ttcggcgtgt tgttcagaag taagtgctga gagaattaca gaataagaga
     3121 ttaatacgtg atgttgatta ttgtgaatca acttaggaag gcaaggatga gcgctacttt
     3181 aacccgcacg ccattgacgt cttcagatcg ttgtgatcga tgtgctgccc aggctcatgt
     3241 tcgcgtatat ctcgaaaatg gttccgcttt acagttctgt ggtcaccact tccgtcaaca
     3301 tgaagttaga ttgcgcagtg tagccgtcga tatccaggac gagcttgcaa agcttgctga
     3361 ccgctaagaa ttaaaaaaca aaatgggcgc cacttcggcg cccattttgt ttttagtcct
     3421 attggaaggt tacatttcct tcttctggaa taagttctac ccaggtattg ccaggtagta
     3481 atccaaccac gtttcctgaa ccatcagtca aagtaattgg tgacttaacg tccggtttag
     3541 accaggtcac atctactttc ttattaccaa gaacagcaaa acctgggcca gtaccttcaa
     3601 gtacagtgcg tggtacgtat ccgcctgcag gatccttgta accggcgtca acagtggtga
     3661 tccgaagaac tactagattc ttggcagaca gttgattgcc tgagatagcc atgtgtggtt
     3721 ttgaaccgtc atcgcgcagc caggttcctg atgtgttatc ccaggtccag tttggtttat
     3781 tcccctttga gaattccaaa cctattttgg caacactctc gcccgcagct gctgctgcat
     3841 tctgagcagc agttggcgca ggtgaagcag aactatcggt gacg
//
LOCUS       DBNHVN010000197         1578 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1150042, whole genome shotgun sequence.
ACCESSION   DBNHVN010000197 DBNHVN010000000
VERSION     DBNHVN010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1150042"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..>1578
                     /gene="der"
                     /locus_tag="AC16Y1_03740"
     CDS             118..>1578
                     /gene="der"
                     /locus_tag="AC16Y1_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012787261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR5831606.1"
                     /translation="MATEEEPLIYEPLGIDAQVDDSIELPENIEDLDEAAYEKVLAQL
                     GLDNEELEEFLAEPQLLPVVAVVGRPNVGKSTLVNRLIGRREAVVEDIPGVTRDRVRY
                     EAEWNGVPFIVVDTGGWEKDAKGMGEQISAQAERAMNTSDVVVFVVDATTGATGTDEA
                     IVRVLRKAKVPVILVANKVDDERAEAEVAQLWSLGLGEPQPVSALHGRGSGDLLDEVL
                     KKFPDVGSMVARPRGPRRVALLGKPNVGKSSLLNILAGEQRSVVSSVAGTTVDPVDEL
                     IELGGQEWILVDTAGIRKKVKQASGHEFYATLRTKSALDKADVAVVLLDASEPMTEQD
                     LRIISMVVESGRALVIAFNKWDLVDEERRYYLEKEIDRQLVQVNWAPRINFSAKTGWH
                     KDRLVPALRAALEGWETRISTGQLNSLFAEIVAAHPHPVRSGKQPKILFGTQASVAPP
                     VFILFTSGFIEAGYRRFVERRIREKFGFVGSPIIINL"
BASE COUNT          413 a          314 c          431 g          420 t
ORIGIN      
        1 cacccgaacc gtttcaccac ttgaaatggc tgctgatgcc aagttagttg acaccaccca
       61 catggaatta gccgaagttg ttgactatgt gttggcatta gtcgaggact taacctaatg
      121 gcaactgaag aagaaccgtt gatttatgaa ccacttggca tagatgcaca agtagacgat
      181 tcgattgagt tgcctgagaa catcgaggat cttgatgaag cagcttatga aaaggtactt
      241 gctcaactag gtctagataa tgaagaactt gaagagtttt tagccgagcc gcagttactt
      301 ccggttgttg ccgtagtagg gcgcccgaat gttggaaagt cgacgctagt taatcgccta
      361 attggtcgac gcgaagcagt tgtcgaagat attccaggtg ttaccaggga ccgcgttcgg
      421 tacgaagctg aatggaatgg tgttccgttc attgtggtcg acaccggtgg ctgggaaaaa
      481 gatgccaaag gaatgggcga acaaatctcg gcacaggctg agcgagcaat gaacaccagt
      541 gatgttgtgg tttttgtggt ggatgcaact actggtgcaa ctggaactga tgaagcaatt
      601 gtgcgggtac ttcgaaaagc aaaagtgccc gttattttgg ttgctaataa agtcgatgat
      661 gaacgtgctg aagcagaagt agcgcaactt tggtctctgg gtcttggtga accccagccg
      721 gtttccgcat tacatggtcg tggttcaggt gacttacttg atgaagtttt aaagaagttt
      781 ccagatgttg gttcgatggt cgctaggccg cgtggcccac gtcgagtggc gctacttggt
      841 aaaccaaatg ttggtaaatc gagtttgctt aatattttgg ccggtgaaca gcgatcagta
      901 gtgagtagcg ttgcaggcac gaccgtcgat ccagttgacg aacttatcga attaggtgga
      961 caagagtgga ttctggttga caccgccggg atccgcaaaa aagttaagca ggcaagtggc
     1021 catgagttct atgcaacttt gcgcactaag tcagctctag ataaagcaga tgtcgccgtg
     1081 gtactacttg atgccagtga accgatgact gaacaagatc tgcgcattat ttctatggtt
     1141 gttgagtctg ggcgtgcact ggtcattgcg tttaacaaat gggatttagt tgatgaagag
     1201 cgacgctatt acttagaaaa agaaatagat cgtcagttag ttcaagttaa ttgggcacca
     1261 cggattaact tcagcgcaaa aactggttgg cataaagaca gattggtacc ggctcttcgg
     1321 gccgcacttg agggttggga aactcgtatt tcaactggtc agttgaattc gctatttgct
     1381 gaaatagttg ccgcacatcc acacccagtg cgaagcggta agcaaccgaa gattttgttc
     1441 ggcacgcaag caagtgtcgc cccacctgta tttatattgt tcacttcagg atttattgag
     1501 gctggctacc ggcgtttcgt cgaacggcga attcgcgaaa agtttggctt tgttggttcg
     1561 ccgataatca taaatttg
//
LOCUS       DBNHVN010000198         1010 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_482570, whole genome shotgun sequence.
ACCESSION   DBNHVN010000198 DBNHVN010000000
VERSION     DBNHVN010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_482570"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..453)
                     /locus_tag="AC16Y1_03745"
     CDS             complement(<1..453)
                     /locus_tag="AC16Y1_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831607.1"
                     /translation="MATSRLNLTRDQLATFLKDHELIKQFERLFATVDVIAPDLVNEI
                     AISAGSAQATANDALALIEALENSLTADGAVTDAKATLALQELQALKSELQLKTIEPV
                     EQYNNSIITDYLTINEHGRGENAVGRIIWDDGEGTIDVGLKGGNVVCKI"
     gene            complement(453..>1010)
                     /locus_tag="AC16Y1_03750"
     CDS             complement(453..>1010)
                     /locus_tag="AC16Y1_03750"
                     /inference="COORDINATES: protein motif:HMM:NF022584.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019073 - viral DNA genome packaging
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein"
                     /protein_id="HIR5831608.1"
                     /translation="RSHQHLYIHLPDRTLVFDAAATEVLGQPVWFCLTSSLVGFSQYK
                     AKNFVWAYNRWLVGDPTSTTIGYLGQEASAHYGQSVRWEFGTTIIYNESKGAIFNQLE
                     LVALTGRVALGENPQISTSYSVDGQTWSQQKFLNAGTIGDRLKRLVWFQQGHMKNWRI
                     QRFQGDSDSHVAFIRLEAQLEPLAF"
BASE COUNT          248 a          250 c          227 g          285 t
ORIGIN      
        1 aatcttgcaa actacatttc caccctttag gccgacgtca attgttcctt ctccgtcgtc
       61 ccaaattatg cggcctacgg cattttcacc gcgcccatgt tcgttgatgg tcaaataatc
      121 tgtaatgatc gagttgttgt attgctccac aggctcaatc gttttgagtt gcagctcaga
      181 cttcaatgct tgcaactctt gcagagcgag cgtggctttg gcgtcagtaa ctgcgccatc
      241 tgctgtgaga ctattttcca gtgcttcaat caatgccagc gcgtcatttg cggtggcttg
      301 agcgctgcca gcagaaatgg caatttcatt caccaagtca ggggcaatta catcaactgt
      361 tgcaaacagt cgctcgaatt gtttaattaa ctcatgatct ttgaggaaag ttgcgagctg
      421 atcacgggtt aaattaagtc ttgatgtagc cattagaatg cgagcggttc aagttgagct
      481 tctagtcgaa tgaaagcgac atgggaatcg ctatcgcctt ggaatcgttg aatgcgccag
      541 tttttcatat ggccttgttg gaaccaaaca agtcgcttta gtcggtcgcc aatcgtgccc
      601 gcattaagga atttttgctg gctccacgtt tgcccatcaa ctgaatatga tgtgcttatc
      661 tgagggtttt cacccaaagc cactcgccca gtaagcgcga ccaattcaag ctgattgaaa
      721 atcgcgccct tgctttcgtt gtaaataatc gtcgtaccaa attcccatcg cacagattgc
      781 ccatagtgcg cgcttgcttc ctgccccaag tatccaatcg tcgtgctagt cggatcgcca
      841 acgagccatc gattgtaggc ccatacaaag tttttcgctt tatattggct aaacccaaca
      901 agtgagctgg tcaaacagaa ccacacaggc tggccgagca cttctgtagc tgctgcatca
      961 aataccaaag ttcgatctgg cagatgaata tataaatgtt gatggctgcg
//
LOCUS       DBNHVN010000199         1024 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1819533, whole genome shotgun sequence.
ACCESSION   DBNHVN010000199 DBNHVN010000000
VERSION     DBNHVN010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1819533"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1024
                     /locus_tag="AC16Y1_03755"
     CDS             <1..>1024
                     /locus_tag="AC16Y1_03755"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0004534 - 5'-3' RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HIR5831609.1"
                     /translation="DQVEALVLTHGHEDHIGAVPYLLRERSDIPLIGSRLTLAFLENK
                     LKEHRITPSTRLVKEGQTTQLGPFNLEFIAVNHSIPDALAVAVKTQAGTVLMTGDFKM
                     DQLPLDGRKTDLNTFARLGDEGVDLFLVDSTNAEVPGFVPSEKDIVPVLDSVMSKASG
                     RLIVASFASHVHRVQQIINLAAEHGRKIAFVGRSMVKNMAVAAELGYLNLPDGLLVTV
                     DEMAKLPDDQAVLICTGSQGEPMAVLSRIANRDHAITAGENDTVILASSLIPGNENAV
                     NRIINELTRHGARVVHKGNALVHVSGHAAAGELLYCYNIVKPKNVMPIHGEWRHLRAN
                     AALAISS"
BASE COUNT          283 a          232 c          233 g          276 t
ORIGIN      
        1 gatcaagttg aagctcttgt tctgacacac ggccatgaag accacattgg ggcagtacct
       61 tatttacttc gcgaacgtag cgacattcca ttaattggct cgcgattaac tctggctttc
      121 ttggaaaaca aactaaaaga acatcggatc acgccaagta ctcgtcttgt taaagaagga
      181 caaacgacac aacttggccc atttaacctt gagttcatcg cggtaaacca ctcaattcca
      241 gacgcacttg cagtggcggt taaaacgcaa gccggaacag tcttaatgac tggcgatttc
      301 aaaatggatc agctacccct tgatggacga aaaacagatc taaatacttt tgccagactg
      361 ggcgatgaag gtgtggactt attcctagtt gattcaacta acgctgaagt tcccggattc
      421 gtccctagtg aaaaagacat agttccagtc cttgattcgg ttatgagcaa agccagtggc
      481 cgattaatcg ttgcctcttt tgcttcacat gtacatcggg tccaacaaat tattaacttg
      541 gctgccgaac atggacgcaa gatcgcattt gttggtcggt caatggttaa gaatatggca
      601 gttgccgcag aacttggtta cctgaacctt ccagatggtt tactagtcac tgttgatgag
      661 atggccaaac tgccagatga tcaagccgtt ttaatttgta ctggctcaca aggtgaacca
      721 atggcggtgt tatcgcgcat tgctaatcgc gatcacgcaa ttaccgctgg tgaaaatgac
      781 accgtgattt tggcctcgtc cttaatcccc ggtaatgaaa acgcagtcaa tcgaattatt
      841 aatgaactga ccaggcatgg tgcacgtgtt gtgcataaag gaaatgcgtt agttcacgtt
      901 tctggccatg ctgcagcagg tgaacttctt tactgctaca acattgttaa acctaagaat
      961 gtaatgccaa tccacggtga gtggcggcat ttgcgagcta atgcagcact tgctatttcg
     1021 tcag
//
LOCUS       DBNHVN010000200         2011 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1151264, whole genome shotgun sequence.
ACCESSION   DBNHVN010000200 DBNHVN010000000
VERSION     DBNHVN010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1151264"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="AC16Y1_03760"
     CDS             <1..626
                     /locus_tag="AC16Y1_03760"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Orn/Lys/Arg family decarboxylase"
                     /protein_id="HIR5831610.1"
                     /translation="RRQLVVNGEQAIGKSIELMNEIRAGIKSGKRFLNAANQISTHPD
                     VLTTDPLRITINTAVGNISGYAARSILFHEHAVHCEMATDHTLVALVGAGATPEVEKF
                     LIALHNLPEQDLTATKVTALPKPGQRELPVRQAYFGDTELVSANLAVGRISADSVAAY
                     PPGIPNLLPGEVITQENIDFLQATVASPFGHVRGGASKDMSVFRVVR"
     gene            complement(636..>2011)
                     /locus_tag="AC16Y1_03765"
     CDS             complement(636..>2011)
                     /locus_tag="AC16Y1_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017945241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase family
                     protein"
                     /protein_id="HIR5831611.1"
                     /translation="SGVSRKAAIQRTLLPVMLEWFAQAPDPDSGLFGFRQVSEALGTT
                     PWYLRLLRDESATAQRLATILATSKYATELLLRAPEAVNLLASQESLKPKSREELLTE
                     INSIASRYEDSTEAVAAIRAVRRRELFRVSCADILGYLAVEKVAQLLTDITDATLSGT
                     LAVVTRSIPSPPPFLIIAMGRYGGCELGYGSDADVMFAYSDANESEMDIQIAHQMANT
                     VRTLLMAPSPDPELLIDADLRPEGKNGPLVRSITSFDSYYQRWSAGWETQALLRAGIS
                     AGDEKLAEEFINLINPLRYRESGLPENELREIRRLKARMEAERLPRGADPTLHTKLGP
                     GGLSDVEWVAQIIQLGYAQKFAQLQTTRTVEALRVAQNLDLLSPEDCSALVDSWLLCT
                     KVRNAVMLVKGKPSDTVPTDLTDLARVAYQLGYGLRGGQQLLEDYRRTTRRARQVVQR
                     VFYGEQD"
BASE COUNT          528 a          469 c          455 g          559 t
ORIGIN      
        1 ctcgtcgtca attagtagta aatggcgagc aggcaattgg taaatctatt gaactcatga
       61 atgagattag agccggaatt aaatctggca aaagattttt aaatgcggca aatcaaattt
      121 caactcatcc agatgtgcta actacagatc cattacggat cacgattaat acggcggtcg
      181 ggaatatttc aggttacgcg gctagatcaa ttttgtttca cgaacatgca gtgcactgtg
      241 agatggcaac tgatcacact ttggtcgcat tagtgggtgc tggtgcaaca cctgaagtag
      301 aaaagtttct cattgccctg cacaatctcc cggagcaaga tttaactgcg accaaagtca
      361 ctgctttgcc taagcctggt caacgtgaac ttccagttcg tcaggcttac tttggtgata
      421 cagaattagt gtctgcgaat ctggcagttg gtcggatctc tgctgatagt gtggcggctt
      481 acccacctgg aattccaaat ttgcttcctg gtgaagtaat cacccaggaa aatatagatt
      541 tcttgcaagc aacagttgcg tccccatttg gccatgtccg gggcggcgca agtaaagata
      601 tgtctgtttt tcgcgttgtt cgctaactta tttttttaat cttgttcacc ataaaaaact
      661 cgctgcacaa cttggcgggc acgtcgagtt gtccgtcgat aatcctcaag taattgttgt
      721 cccccgcgca gaccataacc aagttgatat gcaacgcgag ctaaatcagt taaatctgtc
      781 ggcacggtat cacttggttt acctttaacc agcatcacgg cattgcgaac tttggtacaa
      841 agtagccatg aatctacaag tgctgagcaa tcttctggtg atagcaaatc caggttttgg
      901 gccacccgga gcgcctctac ggttctggtg gtctgtaatt gggcaaattt ttgcgcatac
      961 ccaagttgaa tgatttgtgc gacccattca acgtcactca ggccgccagg tccaagtttg
     1021 gtgtgcaagg ttggatcagc acctcttggt aggcgctctg cttccatccg tgcctttaac
     1081 cgacggattt ctcgtaattc attttctggt aatcctgatt cgcgatatct aagcggatta
     1141 atcaaattaa taaactcttc agctaacttt tcatcaccgg cactaattcc ggcacgcaat
     1201 aacgcttgag tttcccagcc agctgaccag cgttggtagt aagaatcaaa tgaagtgata
     1261 cttcgaacta acggaccatt ttttccctca ggtcgcagat cggcgtctat taacaattct
     1321 ggatcgggac taggtgccat taacaaagtt ctgaccgtat ttgccatttg atgtgcgatt
     1381 tgaatatcca tttcggattc gttcgcatcc gaataggcaa acatcacatc tgcatcgctg
     1441 ccatatccca gttcacaacc accatatcga cccattgcga ttatcaaaaa cggtggcgga
     1501 ctaggtatcg agcgggttac aacagctaaa gttcccgaaa gtgtggcatc agttatgtca
     1561 gtaagtaatt gagcaacttt ttctacagct aagtacccga gaatatcggc gcaacttact
     1621 cggaataatt cccgccgacg aacagcgcgg attgcagcaa cggcttcggt ggaatcttca
     1681 tacctggaag caatcgagtt aatttcagtt agaagttctt cacggctttt aggctttagt
     1741 gactcttgac tggctaataa attaactgct tctggtgcgc gaagtaaaag ttcggttgcg
     1801 tacttactag ttgccaaaat tgtggcaagt cgctgcgcag ttgcagattc atcccgtaac
     1861 aaacgcaaat accaaggtgt ggtgccgagt gcttcactaa cctgccgaaa tccaaaaagc
     1921 ccagagtctg gatcaggtgc ctgggcaaac cattcaagca ttactggcag aagtgttcgc
     1981 tggattgctg cttttcttga aaccccggaa g
//
LOCUS       DBNHVN010000201         1521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1820638, whole genome shotgun sequence.
ACCESSION   DBNHVN010000201 DBNHVN010000000
VERSION     DBNHVN010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1820638"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="AC16Y1_03770"
     CDS             complement(<1..705)
                     /locus_tag="AC16Y1_03770"
                     /inference="COORDINATES: protein motif:HMM:NF016142.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HIR5831612.1"
                     /translation="MGLDISKFVIHPEVKAALADHKAVVALESTIISHGLPSPQNLVV
                     AREIEQNVRDGGAVPATIAIIDGIVRIGLTEQELEILGDPKSNVIKVSTRDLGPIIAI
                     NGHGATTVSATTPLAMLAGIKYFATGGLGGVHRGANETWDVSADLMTLAMNQITIVCA
                     GVKSILDIGATLERLETIGVTVVGYGTDKFPGFYRADSGFGVPWRADTPEEAALIGKR
                     RDGIQAPGAVVIAKPVS"
     gene            756..1235
                     /locus_tag="AC16Y1_03775"
     CDS             756..1235
                     /locus_tag="AC16Y1_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831613.1"
                     /translation="MEYPKIFTAILASLLVVSCSAKSDPEVEGCLSTQTDYQFCVTTE
                     LISQLSSEEMKATDGDFQKEVFEYGAISASTIYYTDLATKQEHILLSVFLFPADKFDA
                     AANPNEPPRFGQEVIRTDGKVLSVAGPQDSMFDPTSQDGMNINALYELMYEAQSWQK"
     gene            complement(1283..>1521)
                     /locus_tag="AC16Y1_03780"
     CDS             complement(1283..>1521)
                     /locus_tag="AC16Y1_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate reductase"
                     /protein_id="HIR5831614.1"
                     /translation="LFGMIGVLGAKIWVENRVDFGDTVNLMIVAATLVIGIADISFTY
                     GDFTFTGIVNGTVVAVVGYQIMRAIAKSRGTTRS"
BASE COUNT          413 a          377 c          317 g          414 t
ORIGIN      
        1 agagactggc tttgctatta ctacggcacc tggtgcttgg attccatctc tacgtttgcc
       61 aatcaacgca gcttcttcag gtgtatccgc ccgccaaggt acgccaaagc cactatcggc
      121 acgatagaac ccagggaatt tatctgtgcc ataaccaacc acagttaccc caatggtttc
      181 taagcgctcg agtgttgcgc caatatccaa aattgattta acacctgcac agacaatcgt
      241 tatttggttc atcgcaagtg tcatcaagtc tgcagacaca tcccacgttt cgtttgcgcc
      301 tcggtgtacc ccgcccagac caccagtagc gaaatattta attccagcaa gcatcgccag
      361 aggggtcgtt gcagatactg ttgtggcacc atgtccgttg atggcaatga tgggacctag
      421 atcacgggtt gaaactttga tcacgttgct cttggggtca cctaggattt caagttcttg
      481 ctcagtcaat ccaattcgga caatgccatc gataatcgca atcgttgccg gcactgcccc
      541 gccatcgcga acattttgtt ctatctctct agctactact agattttgcg gactcggtaa
      601 gccgtgacta atgatggttg attcaagggc gactaccgcc ttgtgatctg caagtgctgc
      661 ttttacttct ggatgaataa caaactttga aatatctaaa cccatgtatt tagcctaata
      721 cccgagtttc ttgtactttc tggcagactt caaatatgga atacccaaaa atctttactg
      781 caattctggc aagtttgctt gtcgtctctt gctcagcaaa atctgatcca gaagtagaag
      841 gttgcttgtc cacccaaact gactaccagt tctgcgtgac tactgagtta atttctcaat
      901 tgtctagtga ggaaatgaaa gccactgacg gcgatttcca aaaagaagtc tttgagtatg
      961 gggctatctc ggcctcaact atttactaca cggacttagc tacgaaacaa gaacatattc
     1021 tgctctctgt tttcttgttc cctgccgaca aatttgatgc ggcagctaac ccaaatgaac
     1081 cgccgagatt tggccaagaa gtaatccgaa ccgatggaaa agtactttcc gttgctggcc
     1141 cacaagattc aatgtttgat ccaacgagtc aggatggcat gaacattaac gcactttatg
     1201 aattaatgta tgaagcacag tcttggcaga aatgatttaa atagaaaagc cgcaggagta
     1261 acctgcggct tttctatttt tactaactac gagtagtacc tcgtgattta gcgattgcac
     1321 gcatgatttg gtagcccaca acagcaacca cagttccatt tacgatgcca gtgaaagtga
     1381 aatcaccata tgtaaatgaa atgtcggcaa tgccgatgac taacgtggca gccacaatca
     1441 ttaggttcac cgtgtcgcca aaatcaactc ggttctccac ccaaattttt gcgcccagca
     1501 caccgatcat gccaaagagg g
//
LOCUS       DBNHVN010000202         1325 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1672734, whole genome shotgun sequence.
ACCESSION   DBNHVN010000202 DBNHVN010000000
VERSION     DBNHVN010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1325)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1672734"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..870)
                     /gene="lexA"
                     /locus_tag="AC16Y1_03785"
     CDS             complement(181..870)
                     /gene="lexA"
                     /locus_tag="AC16Y1_03785"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR5831615.1"
                     /translation="MAKSEDAKVVSLPDGAEGADGLTTRQRLILDMITTTSAERGYPP
                     SVREIAEAVGLASPSSVQHQLGVLQKKGFINRDTSRARALDIRGPEVAGLPEHLTEGD
                     LAPDAAYVPLVGRIAAGGPILAEQSIEEIFPLPKSLVGEGELFMLKVVGDSMVDAAIC
                     DGDFVVVRRQASCDNGDIVAALLDDEATVKTFKKRDGHVWLMPHNPAYEPILGDQATI
                     MGKVVSVLRKV"
     gene            1089..>1325
                     /locus_tag="AC16Y1_03790"
     CDS             1089..>1325
                     /locus_tag="AC16Y1_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831616.1"
                     /translation="MEIKEMALVTAPAALPKQANRPNHLVLVQDYPQKPVKQPIRLTR
                     RGRRVVALLALIPIAVTFLLIGMRSVQASDASAAA"
BASE COUNT          384 a          305 c          295 g          341 t
ORIGIN      
        1 ggctgaacaa aatgggctgc caatcaatga tcgcaaaaat tttggcccaa cagttttagc
       61 tgcatctgta gcttatcttt gcccagagaa cttagaaaaa gttgtagtgc aatagaaaaa
      121 ctaaaaacgc gctgctagag ccaagtctct aatccgcagc gcgttttaat ttacgtaagg
      181 ttaaacctta cgaagtactg aaactacttt gcccataatt gttgcttggt cacccagaat
      241 cggttcataa gcagggttat gtggcattaa ccaaacatgg ccgtcacgtt tcttgaaagt
      301 ttttacagtt gcttcgtcat caagcagcgc agcaacgatg tcgccgttat cgcatgaggc
      361 ttggcggcgc acaacaacaa agtcgccatc gcaaatagcg gcgtcaacca ttgagtcacc
      421 aactactttg agcatgaata gttcaccttc accaacaagt gacttaggaa gtgggaaaat
      481 ttcttcgatt gactgttcag ccaaaatcgg accaccggca gcaatgcgac caacaagtgg
      541 aacgtaggcg gcatctgggg ctagatcacc ttcagttaaa tgttctggta aaccggcgac
      601 ttctggtccg cgaatatcta atgcgcgagc acgggaagtg tcgcggttaa taaaaccttt
      661 tttctgtaac acgcccagtt ggtgttgaac gctagatgga ctggctaaac caactgcttc
      721 agctatttca cgaacacttg gcgggtagcc acgttcagca ctggtagttg tgatcatgtc
      781 gaggataagt cgctggcgag tagttaaccc atcagcacct tcggcaccat cgggaagcga
      841 cacgactttt gcgtcttcgc tcttggccat aactgcctac tttcggtaaa aaccgccaaa
      901 atacggggaa tttcgacgtt ctggtgttaa tttacctgaa atttggaaaa tattcaaaca
      961 tttgttcgaa taaaacttgc tttgtgtcgg aacttaggtg tacaaataga acatacgttc
     1021 taatcgaaca gatgttcgaa aagaaatata taccgaaagc cttaaatcaa ggcttaaggc
     1081 aaaggacaat ggagattaaa gaaatggcac ttgttactgc accagcagca cttcctaagc
     1141 aagctaaccg tcctaaccat ctagtactgg ttcaggatta cccacaaaag ccagttaaac
     1201 agccaattcg actaacccga cgtggccgtc gagtagtggc tttattggct cttatcccaa
     1261 ttgcagtgac ttttctatta ataggtatgc gatcagtcca ggcatctgat gcttcagctg
     1321 cagcg
//
LOCUS       DBNHVN010000203         3396 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_483955, whole genome shotgun sequence.
ACCESSION   DBNHVN010000203 DBNHVN010000000
VERSION     DBNHVN010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3396)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3396)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3396
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_483955"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="AC16Y1_03795"
     CDS             complement(<1..413)
                     /locus_tag="AC16Y1_03795"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR5831617.1"
                     /translation="MNDIYQVTESQFSESELLSEIVEVLKSGQLAVIPTDTSYAVVAD
                     AFHPGAVTLLRMAKQQSPAVPIPVAASSVEMANGIAKLSNLASDLANAFWPGPLTILT
                     RSQPSLTWSISDHRSALSIRVPNSNIAQSLLHRVGP"
     gene            complement(410..1324)
                     /gene="prmC"
                     /locus_tag="AC16Y1_03800"
     CDS             complement(410..1324)
                     /gene="prmC"
                     /locus_tag="AC16Y1_03800"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR5831618.1"
                     /translation="MVTDVRELLSWANTKLTLAKCASPDVDAQLLLAFTLKTDRKSLS
                     AIENLTSAQTEKFKELIEKRVQRIPVQHLTGQAFFRYLELAVGPGVFIPRPETELLVE
                     VGLAELNKQSGAKLAVDLCAGSGAIALSLALECPNTTVHAVEVSAQAFDWLEKNIWAH
                     SEQLARLNSTVITYNDDATDPKVLSALNGTVDVVLSNPPYIPDEMIPRESEVRDHDPK
                     IALFGGEDGLDVARKVSYVAANLLKPQGFFGMEHADVQGKSAVQMLQVMKSENVELWT
                     QVIDQIDYNELPRFVTAVRTSKAILDSQ"
     gene            complement(1324..2406)
                     /gene="prfA"
                     /locus_tag="AC16Y1_03805"
     CDS             complement(1324..2406)
                     /gene="prfA"
                     /locus_tag="AC16Y1_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019354612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR5831619.1"
                     /translation="MSQDFSKVEEMAKEMADLEAKLSDPATHSNPNEARRIGKRHAEL
                     KPVVAKYLELKQLRDDEAAAREMGQEDSAFQAEAEELKIKVEEAAEKLQQMLIPRDPL
                     DSNDVILEIKAGAGGEESALFAGDLMRMYLRYAEKRGWATQVIEAEESDMGGYKDIAI
                     AIKSKGVVPPEEAPYANLKYEGGVHRVQRVPVTESQGRIHTSAAGVLVLPEAEEIDVT
                     IDPNDLRIDVYRSSGPGGQSVNTTDSAVRITHMPTGIVVSCQNEKSQLQNKESAMRIL
                     RARILAAAEEAAAAAASDARKSQVRTVDRSERIRTYNFPENRLADHRVGFKSNNLDQV
                     MDGELDPVISACLEADNKAKLAALGQ"
     gene            complement(2501..2710)
                     /gene="rpmE"
                     /locus_tag="AC16Y1_03810"
     CDS             complement(2501..2710)
                     /gene="rpmE"
                     /locus_tag="AC16Y1_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR5831620.1"
                     /translation="MKAEIHPEYLVTKVTCSCGNTFETRSTQKNGAIHADVCSNCHPF
                     YTGKQKILDTGGRVAKFEKRFGKQS"
     gene            complement(2905..3048)
                     /locus_tag="AC16Y1_03815"
     CDS             complement(2905..3048)
                     /locus_tag="AC16Y1_03815"
                     /inference="COORDINATES: protein motif:HMM:NF040511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPGG-motif small membrane protein"
                     /protein_id="HIR5831621.1"
                     /translation="MDFILWLISIVLIVSGVLKIIRGDLLMGIVFIIVGLLVGPGGTS
                     VFR"
     gene            complement(3178..>3396)
                     /locus_tag="AC16Y1_03820"
     CDS             complement(3178..>3396)
                     /locus_tag="AC16Y1_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831622.1"
                     /translation="NLDERLAQLTGLPATCIPGEMADSPTNTYTGTISQWVNGRYPES
                     TALALELEAEVDRQKIATYAAALQILNN"
BASE COUNT          915 a          788 c          690 g         1003 t
ORIGIN      
        1 ggaccgacgc ggtgtaaaag tgactgagca atatttgaat ttggcactct gatcgacagt
       61 gcagatcgat gatcactaat cgaccaagtc aatgaaggtt gactgcgcgt caaaattgtc
      121 agcggtccgg gccagaaggc attagccaga tcactggcta agttagataa ctttgcgatc
      181 ccatttgcca tttccacaga actcgcagcg actgggattg gaactgcggg cgattgttgt
      241 ttggccattc gaagtaaagt cactgcacca ggatgaaatg cgtccgctac taccgcgtaa
      301 ctagtatctg ttggaataac tgcaagttgt ccgcttttga gtacctcaac gatttcagac
      361 aaaagttcac tttcagaaaa ttgcgattct gttacttgat aaatgtcgtt cattgcgaat
      421 ccaaaattgc ttttgaagtg cgaactgcgg ttacaaatct tggcagttcg ttgtaatcaa
      481 tctgatcaat tacttgggtc caaagttcga cattttcact tttcatgact tgcaacattt
      541 gcactgcaga tttgccttgc acatctgcat gttccatgcc aaagaaccct tgaggtttca
      601 ataagttggc ggccacataa gaaactttac gcgcaacatc aagtccatct tccccaccaa
      661 aaagagcaat ctttggatca tgatctcgaa cttctgattc gcgtggaatc atttcgtctg
      721 gaatataagg tggatttgaa agcacaacat caacagtccc atttaaagcc gataaaactt
      781 tgggatcagt tgcatcatca ttgtaagtaa tcaccgttga atttaacctg gctaattgtt
      841 cgctatgtgc ccaaatattc ttttctaacc agtcaaaagc ttgggcagaa acctcgactg
      901 cgtgcacagt tgtgttggga cattccagtg caaggcttag cgcaattgcc ccagaaccag
      961 cacataaatc gacggctaac tttgccccag attgtttgtt tagttcagcc aaacccactt
     1021 caactaataa ttctgtttct ggtcttggga taaagactcc agggccaacc gcaagttcta
     1081 ggtatcgaaa aaaagcttgg ccagttaaat gttgaactgg aattcgctgg acacgctttt
     1141 caattaattc cttgaatttt tcggtttgag cagacgttaa attttcgatt gcacttaaac
     1201 tttttcgatc tgtcttgagg gtaaaggcta aaagtagttg tgcgtctaca tcagggcttg
     1261 cgcactttgc caaagttaat ttcgtatttg cccaactaag taactcgcga acatctgtca
     1321 ccattactgg cctagcgcag caagtttcgc tttgttatct gcctcgagac aagcactaat
     1381 cacgggatct aactcgccgt ccataacttg atctaagttg ttacttttaa atccaactcg
     1441 atgatccgct aatcgatttt ccggaaagtt ataagttctg attcgctctg accggtcgac
     1501 agttcttacc tgactcttac gagcatctga tgcagcagct gcagctgctt cttcggccgc
     1561 agcgagtatg cgggctcgta gaattcgcat ggcagattct ttattctgca attgtgattt
     1621 ttcattttgg caagaaacta caattccagt tggcatatgc gtgatgcgca cagcggagtc
     1681 tgttgtattt acactctgcc cacctggccc acttgatcga taaacatcga tgcgtaaatc
     1741 atttggatcg attgtgacat cgatttcttc tgcttcggga agtacaagta caccagcggc
     1801 cgatgtgtga atgcgccctt gcgattctgt gactggaact cgttgcacac gatgaacgcc
     1861 accttcgtat ttcaaatttg catatggagc ttcttcaggt ggaactacac cttttgactt
     1921 aattgcaatt gcaatatctt tataaccacc catgtcggat tcttcggctt caattacttg
     1981 agttgcccag cctcgctttt cggcgtagcg caaatacatc cgcattaagt caccggcaaa
     2041 tagtgcagac tcttcgccgc cagcgcctgc tttaatttct aaaattacgt cgttggaatc
     2101 taatggatca cgtgggatca gcatctgctg aagtttttca gccgcttctt ctactttgat
     2161 ttttaattct tctgcctcgg cctgaaaagc tgaatcttct tggcccattt cacgcgctgc
     2221 tgcttcatca tcgcgtaatt gttttagctc caggtactta gccacaactg gcttaagttc
     2281 agcatgacgc tttccaattc gcctggcttc attcggattg ctatgagttg ccgggtcact
     2341 caacttagct tcgagatcag ccatttcttt ggccatctct tcaactttgc tgaagtcttg
     2401 tgacattttt taattcctac taatcaatta actaacgaaa tttggataaa taaataaggt
     2461 gctgccatcg cacctgcgag gacaacacct tattaaaaag ctaggactgt ttgccgaaac
     2521 gcttttcgaa cttagctact cgaccaccag tatcaagaat tttctgctta ccggtataaa
     2581 acggatgaca gttactgcat acgtctgcgt ggattgcacc attcttttgg gtactacgag
     2641 tttcgaatgt atttccgcat gagcaagtaa cttttgttac taagtactct ggatgtattt
     2701 cggccttcac ggatttctcc ctattaataa tgtgctcggg tcgcattaat ttcggacatt
     2761 aagcgtgaac cgaccagcct ctaaattgtg ccagttaaga gcgattaact caaaatttca
     2821 cccaagatga atagccaagg gcgcttttcc ccagacaaac gggaactaaa agcactttgg
     2881 ctgattaact caatgaaaag tgtgttatct gaagacgcta gttccaccag ggccaacgag
     2941 caagccaaca ataataaaaa cgatacccat caatagatca ccgcgaatga tcttcaacac
     3001 tccgcttact ataagcacaa ttgaaatgag ccaaagaata aagtccatga gggactcctt
     3061 tcggatttct aaacacagaa tcacttaact agcgttctat tttcaccata cgccttaaag
     3121 cagaaaaaca acagcagtaa cttaatcaag aaattcgctt tattgttgct aaataaccta
     3181 attatttaaa atttgaaggg ctgcagcata cgtagcaatc ttttgtcgat caacttcggc
     3241 ttcaagttca agagcgagtg cagttgattc agggtatcgc ccattaaccc attgtgagat
     3301 tgttccggtg taagtattag ttggcgaatc agccatctca cctggaatgc aagtggccgg
     3361 caatccagtc aattgcgcta ggcgctcatc aagatt
//
LOCUS       DBNHVN010000204         1415 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1227680, whole genome shotgun sequence.
ACCESSION   DBNHVN010000204 DBNHVN010000000
VERSION     DBNHVN010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1227680"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="AC16Y1_03825"
     CDS             complement(<1..923)
                     /locus_tag="AC16Y1_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR5831623.1"
                     /translation="MAVTIRDVAAHAQVSVATVSRALRGFSSVSQVTRERVETAAAEL
                     DYVLPFRPDFTVHSKAIRVGVVLPYIGRWYFAKVLSGIESVIGDKNGEVSVIRPFDRT
                     GRRKDLADCIDPGSVDAVILVTISITESEFTWLKDRKISVSLLGSTHPEIDNVHIDDF
                     EAMKAATDYLISLNHTRIGMVGTAAFDPAPNQVHVARTNGFIGAMKEANLEVDPELIV
                     QTDFSIKGAQRAVERLFELDNPPTAIVAESDELAFGVITAARDHGIRVPEDLSVTGID
                     DHDVSDSFGLTTVAQPVTSLGEVAAWQVISH"
     gene            961..>1415
                     /locus_tag="AC16Y1_03830"
     CDS             961..>1415
                     /locus_tag="AC16Y1_03830"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR5831624.1"
                     /translation="MGKIAVLGEVVVDRIITDTAKKDIAGGSAANCALALMRKGDDVQ
                     FRARFSTDKDGDFLYQEAIRNQLDVTYSVRTDRPATLVEVRLSATGSPNYQFHLSVTA
                     DWHWTDAELAELDLGSHDAVIYGSLAAILEPNFSRIQEWLRSHNNSNLLV"
BASE COUNT          382 a          331 c          314 g          388 t
ORIGIN      
        1 ctatgagaga taacctgcca ggcagcgact tcacccagtg atgttactgg ttgcgcgacg
       61 gtagttagtc caaaagaatc agaaacatca tggtcgtcaa tcccagttac cgataaatct
      121 tcgggtacac gaataccgtg atctctggct gccgtgataa cgccgaaagc taattcgtcg
      181 gactcggcaa caatcgccgt tggcgggtta tctagttcaa agagtcgttc aacagcacgt
      241 tgtgcacctt tgattgagaa gtccgtttga acaattaact ctggatcaac ttctaaattt
      301 gcttctttca ttgcaccaat aaaaccattg gttcgcgcca catggacttg gttaggtgcg
      361 ggatcaaacg cagccgttcc aaccattccg attcgggtgt gatttaaact aatcaaataa
      421 tcggttgctg ccttcatggc ttcgaagtca tcgatatgaa cgttatctat ttctggatgc
      481 gtcgatccta aaagtgaaac agagattttc ctatctttca gccaagtaaa ctccgattcg
      541 gtaatcgaaa tagttactaa aatgacggcg tctacggagc ctggatctat acagtcggct
      601 aggtctttgc gtcttcccgt tcggtcaaaa ggccgaatca cactaacttc gccattttta
      661 tcgccaatga cactttctat cccgcttaga actttggcga aataccagcg tccgatgtaa
      721 ggaagaacaa cgcctactcg aatagccttt gaatgaaccg tgaagtctgg tcgaaatggc
      781 aacacataat ctaattcggc agcggctgtt tcaacccgct ctcgggttac ttgtgaaacc
      841 gaactaaacc cgcgcagcgc ccgacttact gtagccacac ttacttgagc atgggcagcc
      901 acatcacgaa tagttaccgc cacaaccggc tcctttgtgc catctggtta ttctagcccc
      961 atgggaaaaa tcgcagttct cggtgaagtt gtcgtagatc ggataattac tgacactgcc
     1021 aaaaaggaca tagccggtgg tagcgcagca aattgcgctt tagcattgat gcgaaaaggt
     1081 gatgatgttc aatttcgagc tcgatttagc acggataaag atggcgattt tctctatcaa
     1141 gaggcaataa gaaatcaact agatgtaact tatagcgttc ggactgatcg gcctgcaact
     1201 ttagttgaag tgaggctctc cgccactggt tctcccaatt atcaatttca cttatctgtt
     1261 actgctgatt ggcactggac cgatgcagaa ttggcagaac ttgatttagg ttcacatgac
     1321 gcggttattt atggatcgct agcggcaatt ctagaaccta atttttctcg gattcaggaa
     1381 tggttaaggt ctcacaataa ttcgaattta ctagt
//
LOCUS       DBNHVN010000205         4850 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_39758, whole genome shotgun sequence.
ACCESSION   DBNHVN010000205 DBNHVN010000000
VERSION     DBNHVN010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4850)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4850)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4850
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_39758"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="AC16Y1_03835"
     CDS             complement(<1..617)
                     /locus_tag="AC16Y1_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HIR5831625.1"
                     /translation="MSAVILKRGDIGPAVKEVRDRLVHLGLLATQGSSSEIFDDELFA
                     AIRVFQQSRGLTVDGIVGPRTLRRLDEARWKLGDRVLSYTPGHLIHGEDVAQLQQRLG
                     QLGFAVDRVDGIFGKDTDRIVREFQGNVGLRADGICGPEVFRAIERLERTVTGGSHEH
                     LRELAMWDNFGRTRTLETATVVIEPIANNALLVGQDISIAKVCWD"
     gene            complement(624..944)
                     /gene="trxA"
                     /locus_tag="AC16Y1_03840"
     CDS             complement(624..944)
                     /gene="trxA"
                     /locus_tag="AC16Y1_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009716492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR5831626.1"
                     /translation="MAAVVVDEANFGTEVLTNSKTVLVDFWAEWCGPCRMVAPILDEI
                     AAEHGDKLMIAKLNVDENPGLAANYGVTSIPTLNVYQNGQVVKQIIGAKPKAALLADL
                     AEYI"
     gene            complement(1002..1940)
                     /gene="trxB"
                     /locus_tag="AC16Y1_03845"
     CDS             complement(1002..1940)
                     /gene="trxB"
                     /locus_tag="AC16Y1_03845"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013866149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR5831627.1"
                     /translation="MSAETVDVIIIGSGPSGYTAAIYAARAQLKPIVFEGAVTAGGAL
                     MNTTEVENFPGFPEGIMGPMLMDNMKEQAKRFGAQLITDDVTKVDLTGPVKAVWDGSG
                     KEYRAHSVILAMGSGYRELGIAREKELSGHGVSWCATCDGFFFRDQDIAVVGGGDSAV
                     EEATFLTRFAKSVTLIHRRGELRASKIMAERAHKDPKINFAWNSEVIELHGEPKLSGA
                     TLRDTVTGETRQLAITGLFVAIGHDPRSELIKGQVDLDSEGYVLVKPGSTATNLAGVF
                     ASGDLVDHTYRQAITAAGSGCAAALDAERFLSNLHG"
     gene            complement(2055..3632)
                     /gene="murJ"
                     /locus_tag="AC16Y1_03850"
     CDS             complement(2055..3632)
                     /gene="murJ"
                     /locus_tag="AC16Y1_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR5831628.1"
                     /translation="MAIGTLTSRITGIFRDIALVAAIGTAIFADTYSVANSLPNIIYI
                     LIAGGAINAVFIPALVRHMKDDADGGQKFTDQLLSFIGLILLVIVTLGVIFAGGLVSL
                     YATESWSAQDFKIATLFAMWFIPQVFFYGVYTIASQVLNARDVFVLPMFAPIINNVIV
                     IITALAFLVISDQVPTTDSVNPTQIYLLGAGTTLGVIVQALVLLPALSRAGYRFNPTL
                     NFRGSGLGKIADLAIWTIGFVVVNQVSFLILSNLTTYANVLARNENLVANGFTSYQKA
                     QLMMMLPHSIITVSIVTSLLPRLSKYAHDFDRGNFNEELAKAMRTVVAFIVPLAAILF
                     LAGNRIGQFLYGYGASTAAQGQAVGKVAAMFAIGLPAFSMFYVLLRSYYAREDTKTPF
                     LINVGFNLLHLILGTFLFIRVDTENKVAALAFAYSVSYIAVWLFTWRLVARMDVDLNT
                     AKQTQHLVRVGVAVLIASGIAWASTQLITGFTPENTFGVFLQLVVMATSFFGFYYLAA
                     KQMQITEVSNFKEIVKQ"
     gene            complement(3679..>4850)
                     /locus_tag="AC16Y1_03855"
     CDS             complement(3679..>4850)
                     /locus_tag="AC16Y1_03855"
                     /inference="COORDINATES: protein motif:HMM:NF039636.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HIR5831629.1"
                     /translation="PKSISSVYSQPDVSRLLASSRDEDLINLVVRSQQFSGKNTLVLA
                     PPDGNLTARAINRLGELGVVPIISNEYLTGDLYSNVEAKVVVDQTTSLVSDRATTNCL
                     RSDSDFVDQFQNRNCLISTLTLASIGQIKNSVVLTPADWQPAENELRSLYSELIGKTW
                     LTIAPIAPLNSAQPTSNFAANTQYGVDPFSRELLDSGDSILADAVRTASIFTDLNYAD
                     AFGLARLRGFSSLWESGLQAAEFLVENEFLLSSYQDLVSIESSKNVTVSSGESEIPVT
                     VVNQSDRDITVSIALTSPQTSLFDSPPSGLITVPQGKRVTVPMNVTLASEGILNVTAK
                     LYAPNGQEFGRAKLIQISSAEYQGLARTLVLVAFGLLVLLSISNVVKRRRETTSS"
BASE COUNT         1500 a         1100 c          989 g         1261 t
ORIGIN      
        1 atatcccaac agactttcgc aatcgaaata tcttggccaa ctaaaagtgc attgttagca
       61 ataggttcaa tgactacagt tgcagtttct aaagtacggg tccggccaaa gttatcccac
      121 atagcaagtt cccggagatg ctcatggctg ccaccagtaa cagttcgttc gagtcgctca
      181 atcgccctaa atacctcagg gccacaaata ccatcggctc gaagccctac atttccttga
      241 aattcccgca caatgcgatc ggtgtctttg ccgaaaatgc catcgacccg atcgacggca
      301 aaacccagtt gaccaagccg ttgttgtaat tgagcaacat cttcaccgtg tattaaatgg
      361 ccgggtgtgt aagaaagcac tcgatcgcca agtttccagc gggcttcatc taaacgtcta
      421 agtgtgcgcg gacccacaat tccgtcaaca gttagaccac ggctttgttg aaatacgcga
      481 attgcagcaa atagttcatc gtcgaatatt tcgctcgaac taccttgggt tgctaacagc
      541 ccaaggtgaa cgagacgatc acggacctct ttaactgctg gccctatgtc gccgcgcttt
      601 agtatcacgg cagacattat ttattaaata tattcagcca agtcagccag tagagcagcc
      661 ttaggcttgg cgccgataat ctgcttaact acttgaccat tttggtagac attcaaagtt
      721 ggaattgagg taacgccata gttagcggct agccccggat tctcatccac gtttagtttg
      781 gcaatcatca acttgtcgcc atgttccgct gcgatttcgt caaggattgg ggcgaccatg
      841 cggcacggac cacaccattc agcccagaaa tcaactagaa cggttttgct atttgtcagc
      901 acttcagtgc caaagttagc ttcatcgaca actactgcag ccatcatttc tcctattgtt
      961 gtggtttaag catagatcgt aagtaactca gtagttacga actacccgtg taaattggat
     1021 aaaaatcttt ctgcatcaag cgcagcagca cagcccgagc cggcagcggt aattgcttga
     1081 cggtaggtgt ggtcaaccaa atcgcccgag gcaaaaacac ccgccaaatt tgtggcagta
     1141 gaaccaggct taactagtac gtagccttcg ctatctaaat caacttggcc tttaataagt
     1201 tcagatcttg gatcatgtcc gatggcaaca aataagccag tgattgccag ttggcgagtt
     1261 tcaccagtta cggtgtcgcg cagtgtcgcg ccagataatt ttggctcgcc atgcaattca
     1321 attacttcgc tattccaggc aaaattaatt ttcggatctt tgtgtgcgcg ttctgccata
     1381 atttttgacg cccgcaattc tccgcgtcgg tggattagcg ttaccgattt agcaaaacgt
     1441 gttaggaaag tagcttcctc aacagcagag tcgccaccac caactaccgc gatatcttga
     1501 tcacggaaga aaaagccgtc gcaagttgca caccaagaga caccatgacc agataattct
     1561 ttttcgcggg caatgccaag ttcacgatag ccagaaccca tggccaaaat taccgaatgc
     1621 gctcggtatt ctttgccgga accatcccaa acagctttga ctggtccagt taaatcaact
     1681 ttggttacat catcagtaat taattgagca ccgaaacgct tggcttgttc tttcatgttg
     1741 tccatcaaca ttgggcccat gatgccttca gggaaaccag ggaagttttc aacctcagtg
     1801 gtattcatta aggcaccgcc agccgtaact gcaccttcaa aaacaattgg ctttaactga
     1861 gcacgagccg cataaattgc tgcggtgtag cccgatggac cagaaccaat gatgataacg
     1921 tcgacagttt cagctgacac agagaatcct ttgaattatt aactagcgca aattagttta
     1981 tctagtgcag tagatataac tgtggcaggc cgaatgttat tccagccacg gataaaggct
     2041 tatttactag cgaactattg cttaacgatt tctttaaagt tactcacttc agtaatttgc
     2101 atctgcttgg cggcaaggta ataaaaaccg aagaatgagg ttgccataac tacaagttgt
     2161 aaaaatactc caaaagtatt ttcgggggta aaaccggtaa ttagttgagt acttgcccaa
     2221 gcaataccgc ttgcgattag tacagcaaca ccaactcgaa ctaagtgttg ggtttgttta
     2281 gcagtattta gatcaacatc catcctggcc acaagtcgcc aggtgaataa ccaaactgca
     2341 atgtaagaaa cggaataagc aaaggcaagc gccgcaactt tattttccgt atcaactcga
     2401 atgaataaaa atgtaccaag aattaaatgc aacaaattaa atccgacgtt gattaagaat
     2461 ggggtcttag tatcttcacg cgcgtaataa ctgcgaagta gtacataaaa catagaaaat
     2521 gctggcaacc cgattgcaaa catcgcagca actttgccaa cggcctgacc ctgagccgca
     2581 gttgaagctc cataaccata taagaactgt ccaatacgat taccagctaa aaataaaatt
     2641 gcagctaatg gaactatgaa tgccacaaca gtgcgcattg cctttgccaa ctcttcatta
     2701 aaattgcctc gatcaaaatc gtgcgcgtat ttacttagtc gaggtaaaag ggaagtaact
     2761 attgagaccg taataatcga gtgtggcaac atcatcatca gttgtgcttt ttgatatgac
     2821 gtaaaaccat tcgcgaccaa attctcattt cgcgctaaaa cgtttgcata agttgtcaga
     2881 ttagaaagga ttaaaaaaga aacctgatta actacgacaa aaccgattgt ccagatcgct
     2941 aagtcagcga tttttccaag accactgcca cgaaaattaa gggtcggatt aaatcgataa
     3001 ccagcccgag aaagtgctgg tagcaaaact aaagcctgaa caattacgcc aagtgttgtg
     3061 ccggcaccta ataaataaat ctgggtggga ttaactgaat ctgtcgttgg tacttgatca
     3121 gaaataacta agaatgccaa agctgtgatt atgacgatga cattatttat tattggcgcg
     3181 aacatcggca agacaaatac atctctcgca tttagtactt gagatgcaat tgtgtaaacc
     3241 ccgtagaaaa atacttgcgg aataaaccac atcgcaaata aagttgcaat tttaaaatct
     3301 tgcgctgacc aagattcggt tgcatagaga ctaactaaac caccagcaaa aattacgccg
     3361 agcgtgacaa taactagtaa aataaggcca ataaatgaaa gtaattgatc ggtgaacttt
     3421 tgaccgccat cagcgtcatc tttcatatgt cgaactaggg ccggaataaa tactgcatta
     3481 attgccccgc cagcaatcaa aatataaata atgttgggca aactatttgc aaccgaatat
     3541 gtatcggcaa aaatcgctgt gccgatcgca gctactaatg caatatctcg aaatatgccc
     3601 gtgattcgac tagttaaggt accaattgcc atgcccgaag tttggccaaa ggaattaact
     3661 atattgcgca ctaattacct agcttgaagt agtttctcgt ctgcgtttaa ccacattgct
     3721 tatggacagc agaactagca aaccaaaagc aactaagacc aatgtacggg ccaaaccttg
     3781 gtattcagcg gaagatatct gaatcaattt agctctgcca aactcttgac cgtttggtgc
     3841 ataaagtttg gcagtcacat tcaaaatacc ttcactagcc aaagttacat tcatcggtac
     3901 agttacgcgc ttaccttgcg gcacagtaat caaaccagat ggaggactat caaaaagcga
     3961 agtttgtgga ctcgttagag caattgaaac agtaatgtct cgatcacttt ggttaaccac
     4021 cgtgactggg atttctgatt cgccactaga tacggtgaca tttttagacg attcaattga
     4081 aactaaatct tgatagctac ttaataaaaa ttcattttca actaaaaact cagcagcctg
     4141 tagtccgctt tcccatagag aactaaatcc gcgaagtctg gctaaaccaa atgcatcagc
     4201 ataattaagg tccgtgaaaa ttgatgcggt ccggaccgca tcagccaaaa ttgaatcacc
     4261 cgagtcaagt aactctcgac taaatggatc aacaccatat tgagtattgg cagcaaagtt
     4321 tgaagttggt tgtgcagaat ttaacggtgc gatcggagca atagtgagcc aagtcttgcc
     4381 tattaattct gagtacaacg aacgcaattc attctcagcc ggttgccaat cagccggcgt
     4441 aagaacaact gaatttttga tttgaccaat agaagccaaa gttaaagtac taattagaca
     4501 gtttcgattt tggaattggt caacaaagtc agaatcacta cgtaaacaat ttgtggttgc
     4561 tcggtcgctg actaaagacg ttgtttgatc aacaactact tttgcctcga cgttactgta
     4621 aagatctcca gttaaatact cattagaaat aatcggtacg acgccaagct cacctaaccg
     4681 gttaattgcg cgagcagtta agtttccatc cggcggagct aaaactaaag tatttttgcc
     4741 agaaaattgt tgacttcgaa caactaagtt aattaaatct tcatcacgtg atgaagctaa
     4801 gagtctagaa acatccggct gactatagac actcgaaata gattttggcg
//
LOCUS       DBNHVN010000206         1029 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_630828, whole genome shotgun sequence.
ACCESSION   DBNHVN010000206 DBNHVN010000000
VERSION     DBNHVN010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_630828"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="AC16Y1_03860"
     CDS             <1..291
                     /locus_tag="AC16Y1_03860"
                     /inference="COORDINATES: protein motif:HMM:NF039186.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HIR5831630.1"
                     /translation="VYRRWKRLTLAILPVLIIFTLWDIYAISQGHWTFDPRYVTGIIA
                     VANVPLEEILFFISIPICAVLTLEGVRATKPHWIVGDEKEFISQNSDQARSN"
     gene            291..686
                     /locus_tag="AC16Y1_03865"
     CDS             291..686
                     /locus_tag="AC16Y1_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase domain-containing protein"
                     /protein_id="HIR5831631.1"
                     /translation="MTYTQLALLAVPLAVLVDLYVLRTKLVKRTVFWASYSIIIFFQF
                     LANGVLTGAGIVEYSGAKIIGSDTPVNERPPFLGDGRIAYAPVEDLLFGFAMILLTLA
                     LWVWWGKRGIQREPLAGPPRAIPGWKRNR"
     gene            complement(639..>1029)
                     /locus_tag="AC16Y1_03870"
     CDS             complement(639..>1029)
                     /locus_tag="AC16Y1_03870"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HIR5831632.1"
                     /translation="GVAFQLANFIRDVNEDLDRGRVYLPLSELAKHGVTRADLEKRIA
                     TPQIKAALKEQIARVRQLEEKSKIGISMLSPAAQPCIETARVLYCGIVGAVEEIDFEV
                     FKSRASVPLVKRLAVALPAWYRAWRAR"
BASE COUNT          258 a          219 c          231 g          321 t
ORIGIN      
        1 gtttatcgtc gctggaagcg cttaacgttg gctattttgc cggtactcat tatctttacg
       61 ctttgggata tttatgcaat cagtcaaggg cattggactt ttgacccgag gtatgtgact
      121 ggaattatcg cagtagcaaa tgtgccgctt gaagaaattt tgtttttcat ctcaatccct
      181 atttgtgccg tacttacgct agaaggggtt agagcaacta aaccacactg gattgttggc
      241 gacgaaaaag aatttattag tcaaaattca gatcaggcga ggagtaacta atgacttaca
      301 ctcaattagc gcttctcgcg gttccacttg cggtacttgt tgatctttat gtcctgcgga
      361 ctaaattagt caaacgcact gttttttggg ctagttactc cataattatt ttctttcaat
      421 ttttggctaa cggagtttta actggtgctg gaattgttga atattccgga gcaaaaatta
      481 tcggttctga tacgccggtg aatgagcggc caccatttct aggtgatggc cgaattgctt
      541 atgcaccagt agaagatttg ttatttggtt ttgcgatgat tctgctaact ttggcgcttt
      601 gggtttggtg gggcaagcgg ggcattcaac gcgaaccgtt agcgggcccg ccacgcgcga
      661 taccaggctg gaagcgcaac cgctagtcgc ttaactaatg gaacggaagc tctgcttttg
      721 aagacttcga aatctatttc ttcgacggca ccaacaattc cgcaatagag cactcgcgca
      781 gtttcaatac acggctgtgc tgctggtgac aacatactga tacctatttt tgatttttct
      841 tccaactgcc gcactctggc aatttgttct ttcagagccg ctttgatttg tggagtagca
      901 atacgtttct ctaaatcagc tctagtaaca ccatgtttag ccaattcaga aagtggtaaa
      961 tacacgcgac cacgatctaa atcttcatta acatctctaa taaagtttgc tagctgaaaa
     1021 gctacgcct
//
LOCUS       DBNHVN010000207         1833 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_2044554, whole genome shotgun sequence.
ACCESSION   DBNHVN010000207 DBNHVN010000000
VERSION     DBNHVN010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_2044554"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="AC16Y1_03875"
     CDS             complement(<1..1188)
                     /locus_tag="AC16Y1_03875"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018565076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HIR5831633.1"
                     /translation="MSEVIINTSGMTFEDVIAVARHDAKVVISPEALDAVSKTREHID
                     KLAAATNPVYGVSTGFGALANRHVSPELRKQLQSSLIRSHAAGMGNPVEREVVRALML
                     LRLKTLCSGHTGVRPVVVQTMAQILNAGITPVVYEFGSLGCSGDLAPLAHCAMVLMGE
                     GEVHGTGGQIISAQQALKSAGINPVQLVEKEGLALINGTDGMLGMLIMAITDLEQLCD
                     SVDLTAAMSIEAQLGTDKVFIPELHMPLRPHPGQATSAANIYQALKDSAIVASHENDD
                     TVVQDAYSLRCAPQVAGAVRDTLAHARLVAQRELAAAVDNPVVLEDGRVSSNGNFHGA
                     PVAYVLDFLAIAAADLGSISERRTDRMLDKARSNGLPPFLTDDPGVDSGLMIAQYTQA
                     ALVS"
     gene            complement(1185..>1833)
                     /locus_tag="AC16Y1_03880"
     CDS             complement(1185..>1833)
                     /locus_tag="AC16Y1_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5831634.1"
                     /translation="SNEMLAACAPHSKWIDVFCDRGAFTIEQTRKILSAGIKSGLKPR
                     VHLNQLEQGSGIELAVELDCASADHLTFLTDEDISLLANSQTVAGLVPACDFSTRSAQ
                     YPRARDLLDAGVVVALSPDCNPGSSYTTNMPFSIALAVREMHMSVDEALYAATMGGAK
                     ALRRNDVGQISVGSNADLVLLNASTYVHFAYRPGVPLISKTWKDGNVVFTKGASS"
BASE COUNT          502 a          452 c          391 g          488 t
ORIGIN      
        1 agaaaccaaa gcagcttgag tgtattgcgc aatcattaac cctgaatcga cacctgggtc
       61 atctgtaaga aacggtggta atccgtttga ccgtgcttta tcgagcatgc gatcagttct
      121 gcgctccgaa attgaaccta agtcagctgc agcgattgct agaaaatcta aaacataggc
      181 aactggtgcg ccatgaaaat tgccatttga agatacgcga ccatcttcaa gcactactgg
      241 attatcgacg gcagctgcta attctcgctg cgcaactaat ctggcatgcg ccaaagtatc
      301 gcgaaccgca ccggcaactt gtggggcaca gcgcaatgaa tatgcatctt gtacaactgt
      361 gtcatcattt tcgtgagatg caacaatcgc tgagtcttta agtgcctgat aaatatttgc
      421 cgcactagta gcttgtcccg ggtgtggccg tagtggcata tgaagttcag gaataaatac
      481 tttgtctgtg ccaagttgtg cttcaatact cattgccgca gttaaatcaa cgctgtcgca
      541 aagttgctct aagtcagtaa tcgccataat taacatgccc agcattccgt ccgtgccgtt
      601 aattagtgct aaaccttcct tttcaactaa ttgaactgga ttaattcctg ctgactttaa
      661 tgcctgctgc gctgaaataa tttgcccgcc agtgccatgt acttcacctt cacccattaa
      721 aaccatcgca caatgagcaa gtggggctaa gtcgccactg catcctaggg aaccaaactc
      781 gtaaacgact ggtgtaattc cggcattaag aatctgtgcc atcgtttgca ctacaaccgg
      841 tctcacacca gtatgtccgc tacataaagt tttcaaccta agtaacatca acgcgcgcac
      901 aacttcacgc tcaaccggat ttcccattcc agcagcatgt gagcgaatca atgagctttg
      961 taattgtttg cgcaattctg gtgaaacatg tcgattagcc aacgcgccaa atcctgtgga
     1021 aactccataa accggatttg ttgccgcggc taacttatca atgtgttcac gagttttgct
     1081 aactgcgtcc agcgcctctg gtgagataac aactttggcg tcgtgtctgg caactgcaat
     1141 aacatcttca aatgtcatcc cagaagtatt tatgattact tcactcacga acttgctcct
     1201 ttagtaaaca caacgtttcc atctttccaa gtcttggaaa taagcggcac accaggtcgg
     1261 taggcaaagt gcacataagt tgatgcatta agtagtacta aatctgcatt actaccaaca
     1321 cttatttgtc caacatcatt tctgcgcaaa gctttggcgc cacccatagt tgctgcgtag
     1381 agcgcctcat caacgctcat gtgcatttcg cgcactgcta aggcaatgga aaatggcatg
     1441 ttagttgtgt aactactgcc aggattgcaa tctggagaca gggctacaac tacgccagca
     1501 tcgagtaagt cacgagcgcg agggtattgc gcagaccttg tactgaaatc acatgctggc
     1561 actaatccgg caaccgtttg actgttggcc agcaatgaaa tgtcttcatc ggtaaggaac
     1621 gttaaatgat cggcagaggc acaatctaat tcaacggcca actcaatgcc actaccttgt
     1681 tctagttggt ttaaatgaac tcgaggcttg agccctgatt taattccggc tgacaatatt
     1741 tttcttgttt gctcaatcgt gaaggcaccg cggtcacaaa aaacatcaat ccatttagag
     1801 tggggagcgc aggcagccaa catttcgttt gaa
//
LOCUS       DBNHVN010000208         1626 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1004253, whole genome shotgun sequence.
ACCESSION   DBNHVN010000208 DBNHVN010000000
VERSION     DBNHVN010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1004253"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1626
                     /gene="pheT"
                     /locus_tag="AC16Y1_03885"
     CDS             <1..>1626
                     /gene="pheT"
                     /locus_tag="AC16Y1_03885"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR5831635.1"
                     /translation="EFVKLPEGTTGREVAASLLKVGFEVESVETLGDVRGPLVVGQVQ
                     QIEELTEYKKPIRYCQVNIGGKVNGIICGARNFVEGDLVVVALPGTTLPGDFTITARE
                     TYGKTSNGMICSAREVGLGDEHDGIMVLPAGSAKPGDDAFPILGLGDEVLDISVTPDR
                     GYALSVRGLAREYAIATGLPFSDPGLELPELPQAAGNAVAAASDDRAACDLLVLHTLS
                     NFNPAAPTPDYIRARLAAVGVRSISLAVDVTNYVMFEVGQPLHAFDADKVKGTIRARL
                     AKKKEQLETLDHTMRDLDPEDLVIADDKVALSVAGVMGGAQSEISDTTTRIVIEAAHF
                     NSVTIARSSRRHKLSSEASRRFERGVDRRLAPIAAARATDLLIKHGGATFDGVAGVET
                     APNPTVISFDPALTAKTAGRPYDDTASEAILSSLGCKVDSSKMPWLITAPTWRPDLTM
                     PIDLVEEIIRIDGYEKVPTTVPVGPHGRGLTANQRLLRRIGLHLAGLGLVEVRNYPFI
                     GVSELDALELPKDASRRHAMVLANPLSDEQPML"
BASE COUNT          425 a          367 c          404 g          430 t
ORIGIN      
        1 acgaatttgt taaattaccc gaaggcacaa cagggcgtga agttgccgca agtttgctta
       61 aagtcggttt cgaagttgaa agcgttgaaa ctctcggtga tgtgcgcggc ccactagtag
      121 ttggccaagt tcaacaaatt gaagaattaa ccgaatacaa aaaacctatt cgttattgcc
      181 aagtaaacat tggtggcaaa gtaaatggca ttatctgtgg cgcccgaaac tttgtcgaag
      241 gtgacttagt tgtagtcgca ttgcctggca ctactttgcc aggggatttc accattactg
      301 ctcgcgaaac atatggcaaa acttcaaacg gcatgatttg ttcggctcgc gaagttggac
      361 tgggtgacga gcacgacgga attatggttt tgccagcagg tagcgccaaa ccaggtgatg
      421 atgcatttcc aatccttggc ttaggcgatg aagtacttga tatttccgtc actcctgatc
      481 gtggttatgc gttaagtgtg cgtgggttag ctcgcgaata tgcaattgca accggtttac
      541 cgtttagcga tccaggcctt gagttacctg aactgccgca agccgctggc aatgctgtgg
      601 ctgcagcaag tgatgatcgt gcagcctgtg acttacttgt gctgcacact ttgagtaact
      661 tcaacccagc agcgccaact cctgattaca ttagggctcg attagctgcc gtaggtgtgc
      721 ggtcaatttc acttgcagtc gatgtgacaa attatgtgat gtttgaagtt ggccaaccat
      781 tgcatgcatt tgatgccgac aaagttaagg gcactataag ggcacgccta gccaagaaaa
      841 aagaacaatt agaaactctt gatcacacca tgcgcgacct tgatccagaa gatcttgtga
      901 ttgcagatga caaagttgcg ctttcggtag ccggtgtgat gggcggggcc cagagtgaaa
      961 tttctgacac tacaactcgg attgtgattg aagcagcaca cttcaattca gtgactattg
     1021 cccggtcaag tcgtcgacac aaactttcat ctgaagcatc acgccgtttc gaacgcggtg
     1081 tcgatcgccg cttagcgcca attgctgctg cgcgggctac tgatttatta atcaaacatg
     1141 gtggcgcgac atttgatggg gtagctggtg ttgaaacagc acctaatcca actgtgatta
     1201 gttttgaccc agcgctaact gcaaaaactg ctggtcgacc atacgatgac acagcaagtg
     1261 aagcaatcct ttcttcactt ggttgcaaag tcgacagcag caaaatgcca tggctaatta
     1321 cggcaccaac ttggcgtcca gatttaacta tgccgattga tttagttgaa gaaataatcc
     1381 gcattgatgg ttatgaaaaa gttccaacga ccgtgccagt tggaccacat ggtcgtggac
     1441 tcactgcaaa ccagcggtta cttcgccgaa ttggactgca cttagctggc cttggattag
     1501 ttgaagttcg taactatcct ttcatcgggg taagtgaact tgatgccctt gaattaccaa
     1561 aggatgctag tcgtcgccat gcaatggttt tagctaatcc gctttccgat gagcaaccaa
     1621 tgttgc
//
LOCUS       DBNHVN010000209         1888 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_929673, whole genome shotgun sequence.
ACCESSION   DBNHVN010000209 DBNHVN010000000
VERSION     DBNHVN010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_929673"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..572)
                     /locus_tag="AC16Y1_03890"
     CDS             complement(72..572)
                     /locus_tag="AC16Y1_03890"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017593250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HIR5831636.1"
                     /translation="MSEIIIRAARTTDVRAVRDLVDLYAPGGRLLSKNTVTLYEDVQE
                     FAVAEFNGQIVGCGALHVIWEDLGEVRTIAVLPEHKGKGIGGKILSVLEVKAKELGLK
                     RLFCLTFETDFFGRHGFDEIVEAPVDPTTYAQLLLSGDEGVAEFLDLERVKPNTLGNT
                     RMLKVF"
     gene            complement(574..>1888)
                     /gene="lysS"
                     /locus_tag="AC16Y1_03895"
     CDS             complement(574..>1888)
                     /gene="lysS"
                     /locus_tag="AC16Y1_03895"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR5831637.1"
                     /translation="VGVAGRVMFLRNTGKLCFAQLQAGNGETLQAMLSFDKVGEANLE
                     NWKALVDIGDIVAVEGEVISSKRGELSVLADKWHMAAKALRPLPNPHKPLNEETRVRQ
                     RYADLIMRDTAREVARLRPATLASLRASFAKRDFVEVETPMLQTMHGGAAARPFVTQS
                     NAFDMELFLRIAPELFLKRCVVGGIERVYEINRNFRNEGADSTHSPEFAMLEFYQAYA
                     DYNDMAKLTQELIQEAALAVHGSLTYELVEGGTRDLSGDWDRVEVFPAVSKAVGQEIT
                     PATPIETLRKLCDAAGITYDPKAIHGKLVEELLEHYVVEKFTKPTFVMDYPTDTSPLV
                     RDHRTTKGVVEKWDLYIDGYEAGTGYSELIDPVIQRERLVAQSALKEGGDAEAMQLDE
                     DFLRALEYGMPPSGGVGMGIDRLLMTLTGLNIRETILFPLVKPES"
BASE COUNT          506 a          463 c          399 g          520 t
ORIGIN      
        1 tcaactgaat ataggtgcta actgttccca aaaatcgggg tttttagcca ttggcctacg
       61 cgttaggcga actagaaaac ctttaacatc ctggtatttc caagcgtatt tggcttaacg
      121 cgttctaagt ccaaaaactc ggccacacct tcgtcaccag agagcaacag ctgtgcatac
      181 gttgttggat caaccggggc ttcaactatt tcatcaaatc cgtgccggcc aaaaaagtcg
      241 gtttcaaaag ttaaacaaaa tagtcgcttg agtccaagtt cctttgcttt tacttcaagg
      301 acgctcagaa tttttccacc aatgccttta cctttatgtt ctggtaacac ggcaatagtt
      361 cgaacttcac ctaagtcttc ccagatcacg tgtaacgcac cgcaaccaac tatttggcca
      421 ttaaattcag ccacggcaaa ctcttgtaca tcttcgtaca gagttaccgt attttttgaa
      481 agtaaccggc cacctggagc gtaaaggtca actaaatcac gaacggcgcg cacatcagta
      541 gtgcgagccg cgcggataat aatttcggac aacttagctt tctggtttaa caagtggaaa
      601 caatattgtt tcgcgaatgt tcaatccagt caaagtcata agtaagcgat cgatacccat
      661 tccaactcca ccacttggcg gcataccgta ttcgagtgca cgtaagaaat cttcatctaa
      721 ctgcatggct tcagcgtcgc cgccttcttt gagcgcagac tgggcaacta agcgctcgcg
      781 ctgaattact ggatcaataa gttctgagta accggtgccc gcttcgtaac catcaatata
      841 aaggtcccac ttttcaacca cacctttagt tgtgcggtga tctcgaacca acggcgatgt
      901 atctgttggg taatccatca cgaatgtagg tttggtgaat ttttcaacta catagtgttc
      961 aagtaactct tcgactaact tgccgtgaat cgcttttgga tcataggtaa ttccagctgc
     1021 gtcacaaagt ttgcgcaaag tttcgatggg ggtggccgga gtaatttctt ggccgacagc
     1081 tttacttaca gctggaaaaa cttcaactcg atcccagtca ccggaaaggt cgcgcgttcc
     1141 gccttcgaca agttcgtatg ttagtgaacc gtgtactgca agtgctgctt cttgaattaa
     1201 ctcttgggtt aatttagcca tgtcgttgta atcggcataa gcctgataaa actcgagcat
     1261 ggcaaattct ggtgaatgtg ttgagtcagc accttcattt cggaagttgc gattgatttc
     1321 ataaacccgt tcgattccac caacaacaca acgctttaag aaaagctcag gtgcgatccg
     1381 tagaaataat tccatatcga aagcatttga ttgagtcacg aatggtctag ccgcggcgcc
     1441 gccgtgcata gtttgcaaca tcggagtttc gacctcgaca aaatcacgtt tagcaaaact
     1501 tgcacgcaaa ctagctaaag ttgctggacg taatctagca acttcgcggg cagtgtctcg
     1561 cataattaaa tctgcataac gctggcgaac tcgagtttct tcgttcagcg gtttatgcgg
     1621 atttggtaat ggacgtaatg cttttgctgc catgtgccac ttatcggcaa gaactgaaag
     1681 ttcaccgcgc ttgctgctaa ttacttcacc ttcaacagca acgatgtcac cgatgtcgac
     1741 taacgctttc caattttcaa gattcgcttc gcctactttg tcgaaggaca acatggcttg
     1801 taaggtttcg ccatttccgg cttgcagctg agcaaaacat aattttccgg tattgcgcaa
     1861 aaacattacg cgaccagcaa ctccgacc
//
LOCUS       DBNHVN010000210         4155 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_335869, whole genome shotgun sequence.
ACCESSION   DBNHVN010000210 DBNHVN010000000
VERSION     DBNHVN010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_335869"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="AC16Y1_03900"
     CDS             <1..502
                     /locus_tag="AC16Y1_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020557081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HIR5831638.1"
                     /translation="PCYRREAGSHGKDTRGIFRVHWFDKVEMFSFCSIEEAEAEHKKL
                     LAFEEEFLQALEIPYRVIDVATGDLGSSAARKFDCEAWIPSQERYREVTSTSNCTTFQ
                     SRRLRVRARNNEGIEPVATLNGTLCAITRTIVALFENHQLADGSVRVPAALQPFLGGA
                     KVFKVK"
     gene            complement(553..1929)
                     /locus_tag="AC16Y1_03905"
     CDS             complement(553..1929)
                     /locus_tag="AC16Y1_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HIR5831639.1"
                     /translation="MGSISDDIRTHKYTLTEDEMPTQWYNIIPDLPSPPPPALNPGTL
                     QPAGPEDFAPLFPMELIMQEVSQERYIDIPGGVLDVYRQWRPSPLFRAHRLEAALGTP
                     AKIYYKYEGVSPAGSHKPNTAVPQAYYNAKEGIKRLTTETGAGQWGTALAFACALYGL
                     ECEIFQVGASYDKKPYRRLMIEVFGGKIHRSPSNLTEAGRKLGADPKNQSGSLGIAIS
                     EAVEEAVKDPDTRYALGSVLNHVLMHQTIIGEEALLQLAKAGDTPDLIVGCTGGGSNF
                     GGLAFPFMREKMAGKMSPRILAVEPESCPSLTRGVYAYDFGDTAGMTPLMKMHTLGKD
                     FIPDPIHAGGLRYHGMAPLISHIYELGMMDAIAVPQTECFDAAVLFARTEGIVPAPEP
                     THAIAMAIREANKAKETGESPVIVTALCGHGHFDLAAYDEYLRGKMVDEIISDDRFTA
                     GLANLPQV"
     gene            2020..2832
                     /locus_tag="AC16Y1_03910"
     CDS             2020..2832
                     /locus_tag="AC16Y1_03910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR5831640.1"
                     /translation="MTRLIASDLDGTLLQSSGLVSQENQQAIKRALGEGIDLIFVTGR
                     PSRWMNNIAELTGHTGLALCANGAVLYDLVEEEIVSAQLLPGDVGVLAAQRLRTIDPG
                     ISFAVELARKQDDFLIDHNYRPRWEPKVPPRKVSVEEMFETDLVVKLLARPSSDATFD
                     ADTFLAEADSALDGLVDITHSDNFDVLIEMSLLGVNKGSGLAKVANQRGFSSENVAAM
                     GDMPNDISMIKWAGIGAAVANAHYRVKEVADLHLPTNDEHAFSHLVEHVLSN"
     gene            complement(2829..3755)
                     /gene="pheA"
                     /locus_tag="AC16Y1_03915"
     CDS             complement(2829..3755)
                     /gene="pheA"
                     /locus_tag="AC16Y1_03915"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR5831641.1"
                     /translation="MATKYAYLGPVGTFTEAALIELTKGQDVEYIPAGTVQQALESVR
                     NAEVEGALVPIENSVEGSVSGTVDELSHGELLQIVDEVAIPVEFALLAKPGTKLSEIK
                     KVATHPHAQAQCREWIAKNLPYAHVLPAMSTAAAAAELTDEANYQAAIASVTAAKHYG
                     LVPLAEGIGDKDAAWTRFVLVKKPGQLNPASGHDKTTLALFIKADRPGALLEILTEFA
                     KQNVNLTRIESRPTKKQLGDYFFSIDAEGHIDDENVGNALKALHQTCSDIKFLGSYPK
                     HEEHGVIVDATISELAGVDPDTWLENLRKRNN"
     gene            3846..4121
                     /locus_tag="AC16Y1_03920"
     CDS             3846..4121
                     /locus_tag="AC16Y1_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831642.1"
                     /translation="MDSSVYRRDKDGRIFGTDESQIASARSQQVPLNVQQPVTSNQPI
                     QANKPSVKKIKPQINVPQLRLPSPIKNLLTSNKNKPTTPLSRVTFYA"
BASE COUNT         1145 a          893 c          912 g         1205 t
ORIGIN      
        1 gccttgctat cgacgcgaag cgggcagtca cggaaaagat acacgcggaa tttttcgcgt
       61 gcattggttc gataaagtcg aaatgttttc tttctgtagt atcgaggaag ctgaagctga
      121 acacaaaaaa ttattggcct ttgaggaaga atttttgcaa gccctagaga ttccttatcg
      181 cgttatcgat gttgcaacgg gggatttagg ttcaagcgcg gcaagaaaat ttgattgcga
      241 ggcttggatt ccatctcaag agcgttaccg cgaagtaact tcaacttcta attgcacaac
      301 ttttcaatca cgtcgacttc gtgtacgggc gcgcaacaat gaaggcattg aaccagtagc
      361 tacccttaac ggcactcttt gcgcaatcac gcgcacaata gttgcgttat ttgagaatca
      421 tcagcttgct gatggaagtg tgcgagttcc ggctgcactt caaccattcc ttggtggcgc
      481 aaaggttttc aaagttaaat aatctgcaaa taactaaggc cccgaattcg gggccttagt
      541 tatttaatgt tgctatacct gtggcaaatt agctaagccc gcagtaaagc ggtcatctga
      601 aataatttca tcgaccattt tgccgcgtaa gtattcgtca taagcagcaa ggtcaaagtg
      661 tccatgccca caaagtgctg taacaattac tggtgattcg ccagtttctt tcgccttgtt
      721 tgcttcgcga atcgccattg caatcgcatg agtaggttct ggtgctggaa caattccttc
      781 agtacgagcg aacaaaactg ctgcatcgaa acattctgtt tgtggaactg caattgcatc
      841 catcatgcca agttcgtaaa tgtgtgaaat tagcggagcc ataccgtgat aacgcaaacc
      901 accggcatga attggatctg gaataaaatc ttttccaagt gtgtgcattt tcatcaatgg
      961 ggtcatccct gctgtgtcac caaagtcata tgcataaaca ccacgtgtaa gtgatggaca
     1021 agactcaggt tctaccgcca agatacgtgg gctcattttt ccagccattt tctcgcgcat
     1081 aaatgggaaa gctaagccac caaagtttga accaccacca gtacaaccaa caattagatc
     1141 cggagtatct ccagccttgg ctagttgcaa caatgcttct tcgccaataa ttgtttgatg
     1201 catcaacaca tgatttaaaa ctgaaccgag agcgtaacga gtatcaggat ctttaactgc
     1261 ttcttctacg gcttcagaaa ttgcaatacc taatgaaccg ctttggtttt tcgggtcagc
     1321 accgagtttg cgaccagctt cagttaaatt cgaaggtgaa cgatgaattt ttccaccgaa
     1381 tacctcaatc attaatcggc ggtaaggctt cttgtcataa gaagcaccta cttgaaatat
     1441 ttcgcattca agtccataga gtgcgcaagc aaaggcaagt gcagtacccc attgacccgc
     1501 acctgtttca gtagttagtc gcttgatacc ttctttagcg ttgtaatacg cctgtggtac
     1561 cgcagtgttt ggtttatgtg aaccagcggg tgaaacacct tcgtacttgt aataaatttt
     1621 ggctggtgtg ccaagcgcag cttctaatcg atgtgcacga aaaagtggag atggtcgcca
     1681 ttggcgatat acatctagca cgccaccagg aatatctatg taacgttctt gacttacttc
     1741 ttgcataatc agttccatcg gaaacagtgg cgcaaaatct tctgggccag ctggttgcag
     1801 agttcctgga tttaatgctg gtggcggtgg cgaaggcaga tcagggatga tgttgtacca
     1861 ctgagttggc atttcgtctt cggtcagcgt gtatttatga gtgcgaatgt cgtcgcttat
     1921 gctgcccata atgctccttt agagttggac gaattaagtt agtacttgac tgaaaactct
     1981 agcgagtttt aggcatcttg ataagtgaga gactaaatcg tgacccgttt aatcgcaagt
     2041 gaccttgatg gcactttgct tcaaagttct ggcttagttt ctcaagaaaa tcaacaagcg
     2101 atcaagcgag ctcttggcga gggaatcgac ttaattttcg taaccggccg gccgagtcgc
     2161 tggatgaata acattgccga attaactggg cacaccggtt tagcgctctg tgcaaatgga
     2221 gccgttctat acgacctagt tgaagaagag atcgtcagtg cgcagttatt gccaggcgat
     2281 gtcggtgttt tagctgcgca gcgacttcgc accatcgatc caggaattag tttcgcggtt
     2341 gaacttgctc gcaagcagga cgatttttta attgaccata actatcgacc acgttgggag
     2401 cctaaagttc cgccgcgcaa agtaagtgtt gaagaaatgt tcgaaaccga tttagtcgtt
     2461 aagttattgg ccagaccaag tagtgatgca acttttgatg cagacacttt cttagcagaa
     2521 gcggattctg ccctagatgg tttagttgac attacgcatt cagataattt cgatgtatta
     2581 atcgaaatga gtttgctggg tgtaaataaa ggatctggtt tagcaaaagt tgcgaaccaa
     2641 cgaggattta gttcagaaaa tgttgctgcg atgggcgaca tgccaaatga catttcgatg
     2701 attaagtggg ctggcatcgg tgcagcagtt gcaaatgcac attatcgagt caaagaagtt
     2761 gcagatttac atttaccaac taatgatgaa catgcatttt cacatttagt tgaacatgtt
     2821 ctatcgaatt aattattacg ctttcttaaa ttttctagcc aagtatccgg atcaacacca
     2881 gctaattctg agatagtcgc atcaacaatt acgccatgtt cttcatgctt tggataagaa
     2941 cctaagaact ttatgtcaga acaagtttga tgtagtgcct tgagcgcatt tccaacattt
     3001 tcatcatcga tgtgaccttc agcgtcaatt gagaaaaagt aatcgccaag ttgtttttta
     3061 gtaggcctag attcaattcg agttaagttc acattttgtt tggcaaactc agtcaaaatt
     3121 tcaagtagtg cacctggacg atctgctttt atgaataaag ccaaagtagt tttatcgtgt
     3181 ccacttgctg ggtttagctg cccaggtttt ttgactagaa caaatcgagt ccaggctgca
     3241 tctttatcgc cgatcccttc ggctaacgga actaacccat agtgtttggc tgcagtcacc
     3301 gatgcgatgg cagcttgata atttgcttcg tccgttaatt cagccgcagc tgcagcagtg
     3361 gacattgctg gcaatacatg tgcataaggt aaatttttgg caatccattc acgacattga
     3421 gcttgggcgt gtgggtgcgt ggctactttt ttaatctctg ataattttgt ccccggtttt
     3481 gcaagcagtg cgaattcaac tggaatagcg acttcatcaa ctatttgaag taattcacca
     3541 tgacttaact catctacggt tcctgagaca gaaccttcaa cactgttttc aattggtacc
     3601 aaggcacctt caacttcagc gtttctcaca ctttctagtg cttgttgcac agttccagcc
     3661 ggaatgtatt caacatcttg gcctttagtt aactcaataa gcgcagcctc agtaaacgta
     3721 ccaaccggac cgaggtaagc gtatttcgta gccatcagac tttatagatt acgggcaaat
     3781 tgcagttaac ccaattttgg ttatcaatgc acggataact aatagttata agtgagaatt
     3841 aactagtgga ttcctcggtt tatcggcgcg acaaagatgg ccgtattttt ggtaccgatg
     3901 aatcacaaat tgcctctgca cgaagccaac aagtaccact gaatgttcaa cagccggtaa
     3961 cttcaaatca gccgatccaa gctaataagc caagtgttaa aaaaataaaa ccccagatca
     4021 atgttccaca acttagattg ccttcgccga ttaagaattt attaactagt aataaaaaca
     4081 aaccaactac tcctttgagt cgagtaactt tctatgccta gcgaatagtt atcttttatt
     4141 taattttctt tgtgt
//
LOCUS       DBNHVN010000211         1473 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1598541, whole genome shotgun sequence.
ACCESSION   DBNHVN010000211 DBNHVN010000000
VERSION     DBNHVN010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1598541"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /locus_tag="AC16Y1_03925"
     CDS             <1..911
                     /locus_tag="AC16Y1_03925"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014913352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent malic enzyme"
                     /protein_id="HIR5831643.1"
                     /translation="ALPVMEGKAALFKQFAGVDAWPVCLDTQDVDEIVRTVQIFAPQY
                     GGINLEDISAPRCFEVEERLRNLLDIPVFHDDQHGTAIVVLAALTNALKLVKKKPEDL
                     KIVMSGAGAAGTAIAQLLFEVGMKDITAFDSHGVVTKDRQGDGHTSQWMIEHSNPSGI
                     TGTLADGLVGADVFIGVSAPNIVSEESIASMAKDAIVFALANPDPEIHPALAAKHARV
                     VATGRSDFPNQINNVLAFPGIFRGLLDSGSSKITNETLIAAANAIADCVDSNQLNESY
                     IVPSVFHPDVAKNVAHAVEQVSRNNG"
     gene            904..1374
                     /gene="moaC"
                     /locus_tag="AC16Y1_03930"
     CDS             904..1374
                     /gene="moaC"
                     /locus_tag="AC16Y1_03930"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR5831644.1"
                     /translation="MADLTHLNDDGNAHMVDVAGREVTTRTATAIGKVLLSTEVVKAL
                     RDNSVPKGDVLSAARIAGVMATKKTSDLIPLCHPIAIHGVTVELTVHDNGVVISATVK
                     TADRTGVEMEALTAVSVAGLTVIDMIKSMDPFATITDVQMTEKDGGRNGKWSRG"
BASE COUNT          419 a          293 c          363 g          398 t
ORIGIN      
        1 cagcgcttcc agttatggaa ggtaaagcag cgttatttaa acagttcgcc ggtgttgatg
       61 cgtggccagt ttgtttagat actcaagatg ttgatgaaat agttcgcact gtgcaaattt
      121 ttgcaccgca atatggtggc atcaatttag aagatatttc tgcgccgcga tgttttgaag
      181 ttgaagaacg tctgcgcaat ttattagaca tcccggtttt tcatgatgat caacatggca
      241 ccgcgatcgt tgtgttagcc gcactaacaa acgcactaaa acttgttaag aaaaaaccag
      301 aagatttgaa aatcgtgatg agtggtgctg gcgccgctgg aactgcaatc gcacaacttt
      361 tatttgaagt gggaatgaaa gatatcactg cttttgattc acacggtgta gttactaaag
      421 atcggcaagg cgatggtcat acaagtcaat ggatgatcga gcacagcaat ccaagcggaa
      481 ttactggaac gttagctgat ggtctagttg gcgctgatgt gtttatcgga gttagcgccc
      541 caaatattgt ttcggaagaa tcaatcgcat caatggcaaa agatgcaatt gtgtttgccc
      601 tggctaatcc tgatccagaa attcatccag cactggccgc caaacatgct cgggtagttg
      661 caactgggcg cagcgatttt ccaaaccaaa ttaataatgt gttggcattc ccagggattt
      721 tccgtggttt acttgattct ggaagttcaa aaataaccaa tgaaacattg attgctgctg
      781 ccaatgcgat tgctgattgc gttgatagca accaattaaa tgaaagctac attgtgccaa
      841 gtgtgtttca tccagacgtg gcaaaaaatg tggcccacgc agttgaacag gtttctagaa
      901 ataatggctg atttaactca cctaaatgat gatggcaacg cccacatggt tgatgtggct
      961 gggcgcgagg taacaacacg tactgccacc gcaatcggca aagtattact ttccactgaa
     1021 gttgttaaag cgcttcgcga taactcggta ccgaaaggtg acgtgctttc cgctgctcgg
     1081 attgccggag ttatggccac taaaaaaact tcagatttaa ttccgctttg ccatccaatt
     1141 gcgattcatg gcgtgaccgt tgagttaact gtgcatgata atggcgttgt cattagtgca
     1201 acagttaaaa cagcagatcg aactggtgtc gaaatggaag cacttactgc agttagcgtt
     1261 gctggcttaa ctgtgattga catgattaag tcaatggatc cgtttgcaac aattactgat
     1321 gtgcagatga ctgaaaaaga cggtggccgt aatggtaaat ggagccgagg ctaactttgg
     1381 cgccgcgcac tgctcaagta ataacagcaa gcaatcgggc aagcaaaggt gtctatgaag
     1441 acacaagtgg tgaaattttg gcagctggtt tag
//
LOCUS       DBNHVN010000212         2732 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1896828, whole genome shotgun sequence.
ACCESSION   DBNHVN010000212 DBNHVN010000000
VERSION     DBNHVN010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1896828"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1247
                     /gene="ispG"
                     /locus_tag="AC16Y1_03935"
                     /gene_synonym="gcpE"
     CDS             93..1247
                     /gene="ispG"
                     /locus_tag="AC16Y1_03935"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013154630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR5831645.1"
                     /translation="MSINLGLPALPPPVLAPRRKSRKIVLRHHSNPVEIGGDAPISVQ
                     SMATTLTADVNSTLQQIAELTATGCQIVRVAVPSQDDADALSQIAKKSSIPVVADIHF
                     QPKYVFAAIDAGVAGVRVNPGNIKQFDDKVAEIAKAAGDAGIPIRIGVNAGSLDKRLL
                     EKYGKATPEALVESALWECSLFEEHGFRDIKISVKHNDPVIMVSAYRLLAEQCDYPLH
                     LGVTEAGPAFQGTIKSAVAFGALLSDGIGDTIRVSLSAPPVEEVKVGNQILESLNLRQ
                     RGLEIVSCPSCGRAQVDVYTLAEQVTAGLEGLEVPLRVAVMGCVVNGPGEAREADLGV
                     ASGNGKGQIFVKGEVIKTVPEAQIVETLIEEAMKLAEQMEQTGTPEVSVK"
     gene            1262..2101
                     /locus_tag="AC16Y1_03940"
     CDS             1262..2101
                     /locus_tag="AC16Y1_03940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024708.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5831646.1"
                     /translation="MAHQIRQIDDESINQLIAIADRDPVKHCFLGARLELTRLGMVRP
                     SYPDLIGFFDDGHLKAAIYLGANLIPVNTSAISRNEFANYLIKQGRRCSAIVGPAVEV
                     IPLWQLLEGSWGRERDIRPDQPLMATSSRSSIQPDELVRYATTADLEILFPACVDMFT
                     EEVGISPISGGNSLPYRNRIAELISDKRSFVRIENNEVIFKAEIGTIGNNVAQIQGVW
                     VNPKFRGQGKAVPAMSAVLRFVLDDIAKTASLYVNEFNKPAIATYRRVGFEQVDTFAT
                     VHF"
     gene            2171..>2732
                     /locus_tag="AC16Y1_03945"
     CDS             2171..>2732
                     /locus_tag="AC16Y1_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HIR5831647.1"
                     /translation="MLRMSTLFLRTLREDPADAEVPSHKLLVRGGYVRRIAPGIYSWL
                     PLGYMVYRNIERVVREEMDSAGFQEVHFPGLLPRDPYEATNRWTEYGENLFRLKDRKD
                     SDYLLAPTHEEVFTLMVKSEYSSYKDLPLSIYQIQIKYRDEARPRAGILRGREFVMKD
                     SYSFDTSKDGLVESYNKHRDAYISTFN"
BASE COUNT          769 a          567 c          647 g          749 t
ORIGIN      
        1 aagccgttaa atctctttta acgagttctc ttttagtcgg ttaaggctgg ccgcagcaag
       61 caaggcacca acgtttgccc gataatagaa acatgtcgat taacttgggc ttacctgcat
      121 tacccccacc agttttagcg cctcgtcgca agagtcgaaa aattgtcctt cgtcatcact
      181 cgaatccagt cgaaattggt ggtgatgctc cgataagtgt gcagtcaatg gctacaactc
      241 ttaccgctga tgtgaattca accttgcagc agatcgcaga attaactgca actggatgcc
      301 aaattgttcg cgttgcagta cctagtcaag atgatgctga cgcgttaagc caaattgcga
      361 aaaaaagtag catcccggtt gttgccgaca ttcatttcca acctaaatat gtttttgctg
      421 caattgatgc aggagttgcc ggagtccgag ttaatccggg aaacattaag cagttcgatg
      481 acaaagttgc tgaaattgcc aaagctgcgg gtgatgctgg aattccaatt cgaattggcg
      541 taaatgccgg atctctggat aaacgtcttc ttgaaaaata cggaaaggcc acaccagaag
      601 cacttgttga atccgctcta tgggaatgtt cattattcga agagcatggc tttcgcgaca
      661 taaaaatttc ggtcaaacac aatgatccgg tgattatggt ttccgcttat cgattgttgg
      721 ccgaacaatg tgattacccg ttacatcttg gggtaaccga ggcaggccca gcatttcagg
      781 gaacgatcaa atcagctgtc gcatttggtg cattacttag cgatggaatt ggcgacacaa
      841 ttcgcgtctc gctgtctgcg ccacctgttg aagaagtaaa agttggaaac caaattcttg
      901 aatcgctgaa tttacgtcag cgtggattag aaattgtttc ctgcccatcc tgcggaaggg
      961 ctcaagttga tgtttacacc ttggcagagc aagttactgc aggccttgaa gggctagaag
     1021 ttccattacg ggttgcggtt atgggctgtg ttgttaacgg acctggtgaa gcgcgtgagg
     1081 ctgacttagg tgtagcaagt ggaaatggca aaggtcaaat atttgtgaag ggtgaagtaa
     1141 taaagacggt acccgaagca caaattgttg aaacgttaat cgaagaggcc atgaaacttg
     1201 ctgagcaaat ggaacaaact ggcactccag aagtttcagt taagtaattt ttattacata
     1261 gatggctcac cagattcgtc agattgatga cgaaagtatc aaccagttaa ttgctattgc
     1321 cgatcgcgat ccggttaaac attgtttttt aggtgccagg ctagaactga cccggcttgg
     1381 tatggtcagg cctagttatc cagacttaat tggttttttt gatgatggtc atctgaaggc
     1441 ggcaatatat ctgggcgcca atctgattcc agtaaacaca tctgctatct caagaaatga
     1501 atttgctaac tacttgatta agcaggggcg caggtgttcg gcaattgttg gtccagccgt
     1561 tgaagtcata ccactttggc agctgttaga aggtagctgg ggcagggaac gagatattcg
     1621 cccagatcaa ccactcatgg caacaagttc cagatccagt attcaaccgg atgagttagt
     1681 tcgttacgca actactgcag atctagaaat acttttcccg gcctgtgtcg atatgtttac
     1741 tgaagaagtc ggcatctcgc caataagcgg tggtaactcg ctgccatatc gaaatcgaat
     1801 tgctgaatta atttcagaca agcgttcatt tgttcggatt gaaaataatg aagttatttt
     1861 caaagctgag attggaacca ttggaaataa tgtggctcaa atacaaggag tttgggttaa
     1921 tccaaaattt cgcggccaag gtaaagcagt accggcaatg tcagcggttt tgcgttttgt
     1981 cttagatgac attgcaaaaa ctgcatcttt atatgtgaat gaattcaaca aacctgcgat
     2041 tgccacctat cggcgggtag gttttgagca agttgacacc tttgcaactg ttcattttta
     2101 aatcgcaatt ctcagatttc atgcggtaat caataagatt aaggaacata actacgcaag
     2161 gagtacgact gtgttgcgca tgtccacttt gttcctacga actcttcgtg aggatcctgc
     2221 ggatgccgaa gtacccagtc acaagttatt agttcgcggc ggttacgttc gtcgaatcgc
     2281 gccaggtatc tatagttggt taccacttgg ctatatggtt taccggaaca tcgaacgagt
     2341 ggttcgcgaa gaaatggata gcgcgggctt ccaggaagta cattttcctg gactacttcc
     2401 gcgcgaccca tacgaagcta caaatcgctg gacagagtac ggcgaaaact tatttcgcct
     2461 aaaggatcga aaagactccg actatttatt ggcaccaaca cacgaagaag tattcacttt
     2521 gatggtgaaa agtgaatatt cttcatataa agatctgcca ctttcgatct atcaaattca
     2581 aataaagtac cgcgatgaag cgcggccaag agctggaatc ttgcgcggtc gtgaatttgt
     2641 aatgaaagac tcgtattctt tcgatacttc taaagacggg ttagttgaga gttacaacaa
     2701 acaccgtgat gcatacatct caacatttaa tc
//
LOCUS       DBNHVN010000213         8213 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1080431, whole genome shotgun sequence.
ACCESSION   DBNHVN010000213 DBNHVN010000000
VERSION     DBNHVN010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 8213)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8213)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8213
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1080431"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="AC16Y1_03950"
     CDS             <1..385
                     /locus_tag="AC16Y1_03950"
                     /inference="COORDINATES: protein motif:HMM:NF040429.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6457 domain-containing protein"
                     /protein_id="HIR5831648.1"
                     /translation="QPSQGINASPLKLMTQLNLIGVKVNEGQIQDVDTWADVAKLVKE
                     SSMSEVTPVWLTQVANLLGVSQQDVPVDELLDLTRDVAHNVERKSAPLTTFLIGYAAG
                     KTDSSVSELIDQVSKAIGEWKPDAK"
     gene            360..1211
                     /gene="fdhD"
                     /locus_tag="AC16Y1_03955"
     CDS             360..1211
                     /gene="fdhD"
                     /locus_tag="AC16Y1_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HIR5831649.1"
                     /translation="MSGNQMPSNDLSFDEPVSATARIKVRKTNRDTELTDFVVGEEPL
                     EIRIETNDGKQQLAVTMRTPGADNELGAGFLWSEGLLRSRDDLLAITSCKDVDLTPRE
                     RENIIVAHVKPGAPALTRVLDRRFTISSACGVCGSTQIEDLRERGCQNVEPVGLPVEK
                     LVELPNLMRPRQKIFEKTGGLHAAGLFDPAGNLVVVREDVGRHNAVDKVVGYALMNNL
                     IPLSGYSLAISGRGGYEIIQKAVSAGISAVVAVSAPTSLAVETARAFGLTLLGFTRDG
                     VATQYSS"
     gene            1238..2143
                     /locus_tag="AC16Y1_03960"
     CDS             1238..2143
                     /locus_tag="AC16Y1_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR5831650.1"
                     /translation="MTNTRDEVIPPWLLAVFAMFTVQLGSALSLPVMEKLGAGGTAWL
                     RLSLGGLIFIALGWSALKKLGLRNISNNDWKVLVGLGVTTGLTTVFFLYSIERLPLGT
                     AVAIEFLGPLTVAAVKSHNKKALLWPALALIGVLLLTQPWADEVNLPGIIYALLAGIG
                     WGTYIILTQKIGDRFTGISGLAITIPISAVTAAIIGVPQAVGNITPELLLATLGLAIL
                     LPVLPYALEMLALKHLTHNAFGTLMAMEPGMGLLLGILVLNQIPNFSQWIGISLVIFA
                     GASAQKGGRRVSDNPYSVRENRGHA"
     gene            complement(2323..2398)
                     /locus_tag="AC16Y1_03965"
     tRNA            complement(2323..2398)
                     /locus_tag="AC16Y1_03965"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2363..2365),aa:His,seq:gtg)
     gene            complement(2462..3070)
                     /gene="orn"
                     /locus_tag="AC16Y1_03970"
     CDS             complement(2462..3070)
                     /gene="orn"
                     /locus_tag="AC16Y1_03970"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HIR5831651.1"
                     /translation="MAQTQADRIVWIDCEMTGLDYKNDQLVEIACIITDADLNVVDEG
                     FQVVITTKPEVLAKMDPVVVSMHTESGLITEIPNGVALEQAEAQLFEYISERIPEAGK
                     APLAGSSVHVDRIFLERDMPRVNSYLHYRIIDVSSIKELARRWYPRLYFASPTKVGNH
                     RALGDIKDSINELRYYREVAFIAQPGPDSDQAREVAAKITAN"
     gene            complement(3092..3499)
                     /locus_tag="AC16Y1_03975"
     CDS             complement(3092..3499)
                     /locus_tag="AC16Y1_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831652.1"
                     /translation="MHLGLGKAQSAIRQKSSYDQSVINVSDLLRSAADMLENSQNRVA
                     DQREQSGREVDPQAISDALLMLGATGVSVVRELIESLPDLPRRKSNSSSFSASDVEGM
                     VNQLIARLGLVEESELAALRKRINDLEARINNQ"
     gene            3528..4859
                     /locus_tag="AC16Y1_03980"
     CDS             3528..4859
                     /locus_tag="AC16Y1_03980"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014447435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HIR5831653.1"
                     /translation="MAEIPQYSLTRSARMVSIPAAHAGRRAVGAGKTLLGKDAEEAKL
                     EAQLKTAAQLFRVMGTLKGGAMKFGQIMSVFEASLPEELMKPYRESLSKLQDSAPPMS
                     TANVYKQMKKNLGEDWRSRFIEFNDQPAAAASIGQVHKAIWKDGREVAVKIQYPGAAG
                     ALIADLNQISRLARLIGLTIPGLDVKALVIELKERVIEELDYIHESKAQKQFANAYEG
                     HPDFLIPHVLSAAPEVVVTEWVESRPLLEIINHGSQEERDKYGKLMLRFLLSGPKIAG
                     LLHCDPHPGNFRIAPDGRMVVLDFGATAEFPDGLPDTMGTLLNIAMIGDDAGVVTGLR
                     DAGFIRPNIELNPTSLLNYLAPFTEPTSVPVFKHSRLWLQDLFAKTADPRNPDWSVAF
                     KINLPPEYLMIHRTWVGSLGVLCQLESEFSVSDEFEKWVPAFKDAQRTTRN"
     gene            4937..5009
                     /locus_tag="AC16Y1_03985"
     tRNA            4937..5009
                     /locus_tag="AC16Y1_03985"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4970..4972,aa:Arg,seq:tct)
     gene            complement(5016..5834)
                     /locus_tag="AC16Y1_03990"
     CDS             complement(5016..5834)
                     /locus_tag="AC16Y1_03990"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTD2 family dehydratase"
                     /protein_id="HIR5831654.1"
                     /translation="MEKWAEWIGRTHSQESLLDPLVAQKMQVTINRELSIKSADPLPP
                     AWHWLYFSELTKAQDLGADGHTKLGITLPNFDLPRRMWAGGKITWQRDLILGQVAIRK
                     SQILEIQEKSGRSGKLVFVTLQHEISQNEELCITEIQNIVYREHFKSAQKIEPQQAPT
                     EFDFNESWSLDEVALFRYSALTFNSHRIHYDADYTRQVEGYSGLIVHGPLLATLLLDL
                     AKLNNLPIHEFSYKAVSPITLPTKFFACGKKSETQTDLWISSDSGTLAMQANLI"
     gene            5958..6353
                     /locus_tag="AC16Y1_03995"
     CDS             5958..6353
                     /locus_tag="AC16Y1_03995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR5831655.1"
                     /translation="MNNSTIQAIGNVATEIRFNTTPEGIPVASFRLAATERKFDRETS
                     RWIDGDVNWYTVSCWRALAENVASSVQKGDPVFVLGRLNVRTWERDERSGTSVDITAD
                     IVGHDLARGTSSFKRSTKQVSEEISTAAA"
     gene            6368..8062
                     /gene="ettA"
                     /locus_tag="AC16Y1_04000"
     CDS             6368..8062
                     /gene="ettA"
                     /locus_tag="AC16Y1_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HIR5831656.1"
                     /translation="MAEFIYTLAKARKAHGDKVILDDVTLSFLPGAKIGVVGPNGAGK
                     SSLLKIMAGVDKLSNGDAMLSPGYTVGMLAQEPDLDDTKTVLENVELGVAETKAMLDR
                     FNEISAQMGDPDADFDALMAEMGTLQEQIDHRNAWDLDSQLEQAMDALRCPPSEADVK
                     VLSGGERRRVALCKLLLQQPDLLLLDEPTNHLDAESVNWLEQHLEKYPGTVVAITHDR
                     YFLDNVAQWILELDRGRAYPYEGNYSTYLEKKQARLKVEGAKDIKLQKRLSEELEWVR
                     SNAKARQTKSRARLDRYEEMAIEAEKTRKLDFEEIQIPPGPRLGNVVIEAEDLTKGFG
                     DRILIDNLSFTLPRNGIVGVIGPNGVGKTTLFRMIVAAASGDTSDSATQPDSGKLRAG
                     ETVKLSYVDQSRAGLDPAKNVWEVVSDGLDWIKVGNVEMPSRAYVGAFGFKGPDQQKP
                     SGVLSGGERNRLNLALTLKQGGNVLLLDEPTNDLDVETLGSLENALLEFPGCAVITSH
                     DRWFLDRIATHILAYEGDSQWFWFEGNFESYEKNKIERLGAEAARPHRATYRKLTR"
     gene            8066..>8213
                     /locus_tag="AC16Y1_04005"
     CDS             8066..>8213
                     /locus_tag="AC16Y1_04005"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR5831657.1"
                     /translation="MLEVVVPIHLRWGDMDVYGHINNVAYAQFMEQARVQLMAETGFD
                     PKNFS"
BASE COUNT         2327 a         1668 c         1919 g         2299 t
ORIGIN      
        1 gcaaccatcg caaggcataa atgcttcacc acttaaattg atgacccaac ttaacctgat
       61 cggggttaaa gtgaatgagg gtcaaatcca agatgtcgat acttgggctg atgttgccaa
      121 actagtaaag gaaagttcaa tgagtgaagt aactccagtt tggctaaccc aagttgcaaa
      181 tttacttggc gtatcgcaac aggatgttcc agtcgatgag ctcctagatt taactagaga
      241 tgtagcacat aatgttgagc gcaaaagtgc gccactaact actttcttaa ttggttatgc
      301 agctggaaaa actgactcga gtgtctcgga attaattgat caggttagca aggcaatagg
      361 tgagtggaaa ccagatgcca agtaacgatt taagttttga tgaaccagtt tccgcaactg
      421 cacgaattaa agttagaaaa acaaaccggg ataccgaact tacagatttt gttgtcgggg
      481 aagagccact cgaaattcgg attgaaacca atgatggtaa acagcaactc gcagtaacta
      541 tgcgcactcc gggtgcagat aatgaacttg gtgctggatt tttatggtcc gaaggcttat
      601 taagaagtcg tgatgattta ctcgcaatta cgtcctgtaa agatgttgat ttaacaccac
      661 gagaaagaga aaacataatc gtggcacatg ttaaacctgg cgcacctgcc ttaacgcggg
      721 ttctagatcg taggtttacc atttcaagtg cttgtggtgt ttgtggttca acgcagattg
      781 aagacttgcg cgaacgtggc tgtcagaatg ttgagcctgt tggtttgcca gttgaaaagc
      841 tagttgagct accgaatttg atgcggccac gacagaaaat atttgaaaaa actggtggat
      901 tgcatgcagc cggattattt gatccagctg gaaatttagt agttgttcgc gaagatgttg
      961 gacgccataa cgcagtagat aaagttgtgg ggtatgcctt aatgaataat ctgattccgt
     1021 tgagtggtta tagtttggct atttcaggtc gaggcggata tgaaattatt cagaaagcag
     1081 tgagcgctgg catttctgcc gttgtggcag tttctgctcc tacatcctta gccgttgaaa
     1141 ctgcacgagc ttttggctta accttgttgg gtttcacgcg cgatggcgtg gcaactcagt
     1201 acagttcgta acccaactaa aagaaatttg aaatcctgtg actaatactc gagatgaagt
     1261 catcccgccc tggctgttag ctgtgtttgc catgttcaca gttcaacttg gttcagcttt
     1321 atcacttcca gtaatggaaa aacttggtgc tggtggtact gcctggttac gtctgagtct
     1381 tggtgggtta attttcattg cacttggctg gtctgcgcta aaaaaattgg gtttgcgaaa
     1441 catcagcaat aatgactgga aagttttagt tggcttaggt gtaacaacag gcttaactac
     1501 agttttcttt ttatattcga ttgaacgact gccattaggc acggctgttg caattgaatt
     1561 tctaggtccg ttaaccgttg ctgcagttaa gagtcacaat aaaaaagcgc tactttggcc
     1621 cgcgctggca ttaattggag ttctattact gactcagcca tgggcagatg aagtaaatct
     1681 gccaggaata atttatgcgt tacttgcggg aatcggctgg ggtacttaca taatcttgac
     1741 gcaaaaaatt ggcgatagat ttaccgggat tagcggtctt gcaattacta ttcctatttc
     1801 tgcagttacg gcagcgatta ttggcgttcc gcaggcagtt ggaaacatta ctccggaatt
     1861 actgcttgct actttaggtc ttgcaatttt gttacccgtt cttccatacg cgcttgagat
     1921 gttggcactg aagcatttaa cacataatgc gtttggcacc ttgatggcaa tggaaccagg
     1981 aatgggttta ttgcttggaa ttctggttct aaatcaaata cctaatttca gtcaatggat
     2041 aggtatttcg ttagtgattt ttgccggagc tagtgcgcaa aaaggcggta gaagggtgag
     2101 tgacaatcca tacagcgttc gcgaaaatcg cggacacgca tagaaaatca ctttctttcg
     2161 aaagggattt tcttgaagtc aggtattagg tgactttggt atttaaatgg ggtgagtgac
     2221 gtagcatgca gcgcccgtga gaatcgcggg cccctatgaa aaaacatttt ctttcgaaaa
     2281 tgttttttca aacttaggca tttaactacc tgagtgtttt attggggtga gtgacgggac
     2341 ttgaacccgc gacgtcctgg accacaacca ggtgctctac cagctgagct acacccacca
     2401 tcggttattt agccgaagcg taaacaactt tcccaattgt aatgattcag gcgctgatca
     2461 actaatttgc agtaattttg gcagcaactt cacgagcctg atctgaatct gggccaggtt
     2521 gagcaatgaa agcaacttcg cggtaatacc gtaattcgtt aattgaatct tttatgtcac
     2581 ccagcgcacg atgatttcca actttagttg ggctggcaaa atacagtctt ggataccaac
     2641 gacgggctag ctctttaatc gacgaaacat cgataattcg gtaatgcaaa taagaattca
     2701 ctcgtggcat atctcgctct aagaaaatgc gatctacgtg gactgatgat ccggctaatg
     2761 gagctttacc ggcttcagga atcctttcgc taatgtattc aaataattgg gcttcagctt
     2821 gttcaagcgc cacaccattt ggaatctctg tgatcaagcc tgattcagta tgcattgaca
     2881 caacaaccgg atccattttg gccagcactt caggcttcgt tgttattact acttgaaagc
     2941 cttcgtcgac aacatttaaa tctgcatcgg taattatgca agcgatctcg acaagttgat
     3001 catttttata gtcaagaccg gtcatttcgc agtcaatcca gacaatgcga tcagcttgag
     3061 tttgtgccac gggctttagt ctattaggcg attattggtt atttattctg gcttctaaat
     3121 cattgattcg tttgcgcaac gcagctagtt cagattcttc aactaaacca aggcgagcga
     3181 ttaattgatt aaccattcct tcaacatcac tagcggaaaa ggacgatgag tttgacttac
     3241 ggcgaggcaa atccggtagt gattcgatta attcacgaac gacagaaaca ccagtagcac
     3301 caagcattaa taaagcatca gaaatcgctt gtggatcaac ttcacgacca ctttgttctc
     3361 gctggtcggc aactcgattt tggctgtttt ccagcatgtc agctgcgctg cgcagtaagt
     3421 cagaaacgtt aatcacactt tgatcgtaac ttgatttttg tctaatcgca ctttgagctt
     3481 tgcctaagcc aagatgcatc cacttcagtt ttaatagaca atgaattgtg gctgaaatcc
     3541 ctcaatattc actaacccgc agtgcgcgca tggttagtat tccagccgcg catgcagggc
     3601 gtcgtgcagt tggcgcgggc aaaaccttgc ttggtaaaga tgccgaagaa gccaaactag
     3661 aagcacaact gaaaacagcc gctcaacttt tccgggtgat gggaacactc aaaggtggcg
     3721 cgatgaaatt cggccagatt atgagtgttt ttgaagcttc acttcctgaa gagttaatga
     3781 agccgtatcg cgaatcacta agtaagttac aagattctgc gccaccaatg tctaccgcca
     3841 atgtttataa acaaatgaaa aaaaatcttg gtgaagactg gcgttcacgt tttattgaat
     3901 ttaatgatca accagctgct gcagcctcaa ttggtcaggt gcataaagct atctggaaag
     3961 atgggcgcga agttgcggta aaaattcagt atcccggagc agccggcgca cttattgctg
     4021 acttaaacca gatttcacga ttggctcgac ttattgggtt aactattccc gggcttgatg
     4081 taaaagcctt ggtaattgaa ttgaaagagc gtgtaattga agagcttgac tacatccatg
     4141 aatctaaggc gcaaaagcaa tttgcaaatg cctatgaggg tcatcccgac tttttaatcc
     4201 cgcatgtatt gtccgcagca cctgaggttg tagtaaccga atgggttgaa tcgcgtccgt
     4261 tacttgaaat catcaatcat ggttctcaag aagagcgaga taaatacgga aagttgatgt
     4321 tgcgcttctt gctttctgga ccaaaaatag cgggcttatt acattgtgat ccgcacccag
     4381 gaaacttcag aatcgccccc gatgggagaa tggtggttct tgattttggt gcaactgcag
     4441 aatttccaga tggactacca gacacaatgg gcacactctt aaatattgcg atgatcggtg
     4501 atgacgcagg agtagttact ggtttacgtg acgctggatt tattcgacca aatattgaac
     4561 taaatccaac ttcgctgctt aactacttag caccgttcac cgaaccaact tcagtgccgg
     4621 tgtttaaaca ttctcgactt tggttgcaag atctattcgc aaaaactgct gacccaagaa
     4681 atccagattg gtcagtggct ttcaaaatca acctgccgcc tgagtatttg atgattcacc
     4741 gaacttgggt gggcagcctc ggtgtgcttt gccaattgga atcagaattt tcggtaagcg
     4801 atgagttcga aaaatgggtg cctgccttta aagacgcaca aagaacaact agaaattaga
     4861 aattgattta caccgaatta tcggcaaatt caggcaagaa tttggcaagt acaattaggg
     4921 cggaacattt agtccagcgc tcgtagctca atggatagag caacaggttt ctaccctgtc
     4981 ggctgggggt tcgagtccct ccgagcgcgc attacttaaa ttaaatttgc ttgcatcgca
     5041 agtgttcccg agtcgctact tatccataaa tcagtttgag tttctgattt tttaccacaa
     5101 gcaaaaaatt ttgtaggcaa agtgattggg ctaacggctt tgtatgagaa ctcatgaata
     5161 ggcaagttat ttaatttagc taaatctaat aacaacgtgg caagtaatgg accatgcaca
     5221 atcaatccgg aatagccttc aacttgccgg gtgtagtccg catcgtaatg tattcgatga
     5281 ctattaaaag taagtgctga gtaacgaaat aaggcgacct catctagaga ccaagattca
     5341 ttgaaatcga attcagttgg cgcctgctgt ggttcaattt tttgagcaga tttgaaatgt
     5401 tcacgataaa caatgttctg aatttccgtt atacaaagtt cttcattttg agaaatttca
     5461 tgttgcaaag tgacgaaaac taattttccg ctgcgacctg atttctcttg aatttctaaa
     5521 atttgtgact ttcgaatagc aacctgaccc aaaattaaat cgcgctgcca agttattttt
     5581 ccaccagccc acattcttcg aggtaaatca aaattgggta aggtaattcc gagtttggtg
     5641 tgaccatccg cgccaagatc ttgtgctttg gtgagttcag aaaaataaag ccaatgccac
     5701 gctgggggta gcgggtcagc ggacttgatt gataattctc gattgatcgt cacttgcatt
     5761 ttttgggcaa ctagtggatc aagtaatgac tcttgcgaat gagttcgtcc aatccattca
     5821 gcccactttt ccatgtaacc gagcctaatt ggatatggaa tctggtatcc acaacggtag
     5881 gaactgcaca gttaaaaata gttcattacc taatcactcc tgccaccaca caatccttaa
     5941 aaaattaagg agtcagtatg aataactcaa caattcaggc gattggcaat gtcgcaacag
     6001 aaataagatt caacaccaca cctgaaggta ttccggtagc tagtttcaga ttggccgcaa
     6061 ctgaacggaa atttgatcga gaaacttcac ggtggataga tggtgacgtc aattggtaca
     6121 cagtttcgtg ttggcgggca ctagctgaaa atgttgccag tagcgttcaa aaaggagatc
     6181 cagtatttgt tctgggccgg ttaaatgttc gcacctggga gcgagatgag cgcagtggaa
     6241 catcggttga catcacagca gatattgtgg gacatgatct tgccagagga acttcgagtt
     6301 ttaagcgctc aactaagcaa gtatctgagg aaataagtac cgctgctgcc taagtagaat
     6361 tgagcccatg gctgagttca tttataccct ggcaaaagcg cgcaaagcac atggcgacaa
     6421 agtaattctt gatgacgtaa cactttcttt tctacctggc gcaaaaattg gcgttgttgg
     6481 tccgaacggt gccggtaaat caagtttgct aaaaatcatg gctggcgttg acaaattatc
     6541 caatggtgat gcaatgctca gcccgggcta caccgttggc atgttagctc aagaaccaga
     6601 tctagatgac actaaaactg ttttagaaaa tgttgagctt ggtgttgcag aaacaaaagc
     6661 aatgcttgat cgattcaatg aaatctcggc gcagatgggt gatccagatg cagactttga
     6721 tgcgctcatg gcagagatgg gaactctgca agagcagatt gatcaccgca atgcttggga
     6781 tttagatagt cagttagagc aggccatgga cgcattgcgt tgtccaccta gtgaagctga
     6841 tgtaaaagta ctttccggtg gtgaacgccg tcgcgttgca ctatgtaaat tacttttgca
     6901 gcaaccagat ttattactgc ttgacgaacc aactaaccac ctagacgcag aaagtgttaa
     6961 ctggttagaa cagcacctcg agaagtatcc aggaactgta gtagcgatta cgcacgaccg
     7021 ttacttcctg gacaatgttg cgcaatggat tttggaattg gaccgtggac gggcatatcc
     7081 atacgaaggt aactactcaa cttacttgga aaagaaacag gctcgcttaa aagttgaagg
     7141 tgccaaagat atcaagttac aaaagcggtt atctgaggaa cttgaatggg ttcgctcaaa
     7201 tgccaaagct agacagacta aaagtcgagc tcggttggat cgctatgaag aaatggcaat
     7261 cgaagcagaa aaaactcgca aattagattt tgaagaaatc caaattccac ctggtccacg
     7321 cttaggcaat gtggtcattg aggcagaaga tttaactaaa ggttttgggg atcgtatttt
     7381 gattgacaac ctttcgttta ccttgccaag aaatggaatt gtcggtgtga ttggcccaaa
     7441 cggtgttggt aaaacaacgc tatttcgaat gattgttgcc gcggcttcag gtgatacatc
     7501 tgacagcgct actcaacctg actcaggaaa acttcgtgct ggtgaaacgg taaaactctc
     7561 atacgttgac caaagccgag ctggtttaga tccagcaaag aatgtttggg aagttgtttc
     7621 agatggtttg gactggataa aagttggcaa tgttgaaatg ccatcgcggg cgtatgttgg
     7681 cgcatttggc ttcaaaggtc cggaccagca aaaaccttct ggagttcttt ccggtggtga
     7741 acggaaccga ttgaatttag cattaacgct gaaacaaggt ggaaacgttt tgctacttga
     7801 cgaaccaact aacgacttag atgttgaaac cttgggttcc ttagaaaatg cgttacttga
     7861 atttcctggc tgtgctgtaa ttacttcaca cgaccgctgg tttttagatc gcattgctac
     7921 acacattttg gcttatgaag gcgactctca atggttctgg tttgaaggta actttgagtc
     7981 ttatgagaaa aataaaatcg agcgcttagg ggccgaagca gcaagaccgc atcgggctac
     8041 ttatcgaaag ttgacccgct agttaatgct tgaggtagtt gttccaattc atctgcgctg
     8101 gggtgacatg gatgtttacg gccacattaa taatgtggcc tatgcccaat ttatggagca
     8161 agctcgcgtt caattaatgg ctgaaactgg ttttgatcca aaaaattttt ctg
//
LOCUS       DBNHVN010000214         5381 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1822727, whole genome shotgun sequence.
ACCESSION   DBNHVN010000214 DBNHVN010000000
VERSION     DBNHVN010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5381)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1822727"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="AC16Y1_04010"
     CDS             complement(<1..214)
                     /locus_tag="AC16Y1_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019058652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5831658.1"
                     /translation="MDDINSGVGVLDKAAHILDTLESGPLTLAGLVSATGLARPTVHR
                     LAMALEHHRYLTRDLSGRFTLGPRFAE"
     gene            273..1691
                     /gene="leuC"
                     /locus_tag="AC16Y1_04015"
     CDS             273..1691
                     /gene="leuC"
                     /locus_tag="AC16Y1_04015"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR5831659.1"
                     /translation="MGRTLAQKVWDDHVVRSAPGEPDLLFIDLHLVHEVTSPQAFDGL
                     RAAGRKVRRTDLTLATEDHNVPTIDIDKPIADPISRIQVDALRSNCAEFGVPIYSLGN
                     QEQGIVHVVGPQTGLTQPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLPL
                     KPFKTMAITVNGQLPDGVTAKDLILTIIAKIGTGGGQGYVLEYRGEAIRSLSMEARMT
                     ICNMSIEAGARAGMIAPDETTFAYLKGRERAPKGADWDKAVAYWQTLPTDSDAVFDRE
                     VIIEASELSPFVTWGTNPGQGVPLNATVPSPDDFTDPVDKAACEKALAYMDLKAGQKI
                     KDIRIDTVFLGSCTNGRIEDLRAAAEILRGQKIADNIRMMVVPGSVRVRLQAMEEGLD
                     KVFTDAGAEWRGAGCSMCLGMNPDKLTPGERSASTSNRNFEGRQGPGGRTHLVSPQVA
                     AATAIRGTLSAPSDLDQLMSVS"
     gene            1694..2281
                     /gene="leuD"
                     /locus_tag="AC16Y1_04020"
     CDS             1694..2281
                     /gene="leuD"
                     /locus_tag="AC16Y1_04020"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR5831660.1"
                     /translation="MEKFVKHTGTAVPLRASNVDTDQIIPAEYLKRISRHGFEDGLFS
                     AWRKDENFILNQPQFKNGTVLVAGPDFGTGSSREHAVWALMDYGFRVVISSRFADIFR
                     GNSGKQGLLTAVVDQDFVDQLWKEIENHPDTQVTVDLESRTITAGSLTSKFEVDDYVR
                     WRLMEGLDDVGLTLKQVDSIDQFEANRPSFKPVTA"
     gene            2510..2788
                     /locus_tag="AC16Y1_04025"
     CDS             2510..2788
                     /locus_tag="AC16Y1_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003215863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR5831661.1"
                     /translation="MNKAELIDAVAEAAGLSKKDATAAVDGVFDTITRAVAKGDKVSL
                     TGFGIFEKSDRAARTGRNPQTGAPVQIAATSVPKFRAGAEFKKKVSGK"
     gene            3063..3806
                     /locus_tag="AC16Y1_04030"
     CDS             3063..3806
                     /locus_tag="AC16Y1_04030"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR5831662.1"
                     /translation="MGKSLKPKSTGIWYRIIVGLLRPFMTLLTKRDWRGGRQLQNYQG
                     VVVAMNHLSWFDPFASGHFVHDHGRQVRFLAKAEVFKLPGIGRVLKSAGQIPVNRQDS
                     DAAAGSLRIAMEAVRRGECIVIYPEGTLTRDPDLWPMSAKTGAARIALTTGVPIVPAA
                     QWGPQEVLAPYGKVFKIFPRKTMHIWAGPPVDLEDLRSQPITAATLREATDRIMRDIT
                     KLLGEQRGLTPPDNPMPRNVVPPRKAASA"
     gene            3803..4825
                     /locus_tag="AC16Y1_04035"
     CDS             3803..4825
                     /locus_tag="AC16Y1_04035"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016571146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR5831663.1"
                     /translation="MKRVAVMGAGSWGTVFSVILADAGCEVSLWARSTQLADEINSSR
                     VNRFYHPNLKLPAAVTATANPDEALANSELVILAVPAQTLRENLGAWAISIPTEAVIC
                     SLIKGVELGTAERMSEVIAEVAKVDINRVAVISGPNLAHEIAMREPAATTVACTDEQS
                     ARRIQDAVTTDYFRPYYTTDVIGVEIAGAVKNVIALANGMAVGLGYGENSQAAIMTRG
                     LAEMTRLGVALGANPLTFAGLAGVGDLIATCQSPLSRNRTIGEHIGKGMSVEEAQLVT
                     LTTSEGVKSCRSILDLANKHKVEMPITEQVVNVIHHGRNANDVLRAFMSRSTRAEEGI
                     EKMEHH"
     gene            4882..>5381
                     /locus_tag="AC16Y1_04040"
     CDS             4882..>5381
                     /locus_tag="AC16Y1_04040"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HIR5831664.1"
                     /translation="MANRIRVAVIFGGRSSEHTISCISAGSVLRAIDQNKYEVVPIGI
                     THEGKWVLESADSARLSVTDGVFPAVDSSRSPILFTPDPSATHLVVQSEVPDVLAQVD
                     VVFPVLHGPWGEDGTIQGLFELAGVPYVGSGVFASAAAMDKGHLKALMKAAGIPIGPY
                     EVVTDA"
BASE COUNT         1486 a         1158 c         1354 g         1383 t
ORIGIN      
        1 gttctgcaaa acgtgggcca agggtgaagc gtccagaaag gtcccgagtt aagtaacgat
       61 ggtgctctag cgccatagcc aaccggtgca cggtgggacg agccaaacca gttgctgaaa
      121 ctaaaccagc caaagtcagc ggacctgatt ctaaggtgtc caaaatgtgg gcagccttgt
      181 caagaacacc gacaccgcta ttaatatcgt ccatgtagtg atattgctat ctcgaataat
      241 gagatgtcaa tttaggtcaa ggagtcacgg gtatgggacg cactttagcc cagaaagttt
      301 gggatgatca cgtagttcgc agcgcaccag gcgaaccaga tctattgttc atcgacctgc
      361 acctggtaca cgaagtaacc agcccacaag cattcgatgg attgcgcgct gctggccgca
      421 aagtgcgccg cacagattta actttggcca ccgaagatca caatgtgcca actatcgata
      481 ttgataagcc gattgcagat ccaatcagcc gaattcaagt tgatgccctg cgtagtaact
      541 gcgctgagtt tggcgtaccg atttattcac ttggtaatca agaacaagga attgtgcacg
      601 tagttggacc ccagacaggt ttgactcaac caggcatgac aatagtttgc ggtgactcac
      661 acacttcaac acatggtgcg tttggcgcat tagcttttgg tatcggtact agcgaagttg
      721 aacacgtact tgctacccaa acacttccgc taaagccttt caaaacaatg gcgatcactg
      781 taaatggtca attgcctgac ggcgtcaccg ctaaagattt aattttgacg atcatcgcca
      841 aaattggaac tggcggtggc caaggttatg ttctggaata tcgtggcgaa gcaatccgaa
      901 gtctttcaat ggaagcgcgc atgacaatct gcaatatgag tatcgaagca ggtgcgcgcg
      961 ctggcatgat cgctccggat gaaacaactt ttgcttatct aaagggtcgc gaacgcgcac
     1021 ctaagggtgc ggattgggat aaggcagttg cttattggca gaccctgcca actgattcgg
     1081 acgcagtatt tgatcgtgag gtcattatcg aggcaagcga actaagccca tttgttactt
     1141 ggggaacaaa cccaggtcaa ggtgttccgc ttaatgcaac cgtgccttca cccgatgact
     1201 ttactgatcc agtcgataaa gcggcttgtg aaaaagcact tgcctatatg gatttaaaag
     1261 ctggtcaaaa aattaaagac ataagaatag acactgtatt tttgggttcg tgcactaatg
     1321 gtcgaattga agatttacgt gcggccgcag agattttgcg cggccagaaa attgcagaca
     1381 acattcgtat gatggttgtg cccggatctg ttcgggtgag actgcaagcg atggaagagg
     1441 gcttggataa agtctttacc gatgcgggcg ccgaatggcg tggggcaggt tgctctatgt
     1501 gtcttggaat gaatccagac aaactaactc caggtgaacg aagtgcatct acctcgaacc
     1561 gcaacttcga agggcgacaa ggcccaggtg gtcgcactca cttagtttca ccgcaagtcg
     1621 ctgcggcaac tgcaattcgt ggaaccttat cggcaccaag tgatctagat caattaatga
     1681 gcgtgagtta ataatggaaa agtttgttaa gcacactggt acagctgttc cattgcgggc
     1741 atcaaatgtc gatactgacc agataattcc ggctgaatat ttaaagcgca tttcaaggca
     1801 cggcttcgaa gatggccttt tttcagcttg gcgcaaagat gaaaacttca tacttaatca
     1861 gccgcagttt aaaaatggca cggttttggt agctggccct gattttggca ctggctcaag
     1921 ccgtgagcat gcagtttggg ctttgatgga ctatggattt cgcgtggtga tttcatctag
     1981 atttgccgat attttccgag gcaattcagg caagcaaggg ttactgactg ctgtagtaga
     2041 tcaagatttt gtagaccaac tatggaaaga aattgagaat catccagaca cccaagtaac
     2101 agttgatcta gaaagtcgca ctattactgc gggaagtttg acctcaaagt ttgaagttga
     2161 tgattacgtg cgctggcgat taatggaagg ccttgatgac gttggattaa cgttgaaaca
     2221 agtcgattcc atcgatcaat ttgaagccaa tcgcccaagc tttaagccag taactgctta
     2281 ggtttttgtg tggataagta ggtatttacc cgcaaaacag caaaaaaacc caagaaattg
     2341 ggattttttg ctgtttttgg gcgtgtcact tagaaaaaac cttgattttg cgccgtaaag
     2401 tcgagattga gttggacggg gtagtccaac ccaactaatt agccaaaggt taattagcca
     2461 acagaaagcc ggctttcgct tgattgtcgg attaaatgga ggatctcaag tgaacaaggc
     2521 agaacttatt gatgcagttg cagaagctgc aggcctaagc aaaaaagatg caaccgctgc
     2581 agttgacggt gttttcgaca ccattactcg tgcagtagcc aagggtgaca aggtttccct
     2641 aactggcttc ggtatcttcg aaaaatcaga ccgcgcagca cgtactggcc gcaacccaca
     2701 aactggtgct ccagttcaga ttgcagcaac atcagtacct aagtttcgcg ctggcgctga
     2761 atttaagaag aaagtatctg gcaagtaagc caacttactt atttaataaa gcccgctggg
     2821 aaactggcgg gctttattta ttgccgagta tttttggttt aggtgcttat cttccgaaag
     2881 acttacttca aaacccagaa ttttgggttt tcccgatgat tggttgggta acctgatttc
     2941 tggctatatg tactgtgaca gaatttagtt agcaagaaac ttagttagta cgaaatttag
     3001 ctagcaagaa tcccaaccag ataccaaagc aagtaatact tagtcgaaaa gtgaagcagg
     3061 cagtgggcaa aagcttaaaa ccaaagtcaa ctgggatttg gtatcgaatt attgttggct
     3121 tgttgcgccc attcatgact ttattaacca aacgggattg gcgcggcggc aggcagctgc
     3181 aaaactacca gggcgttgta gttgccatga atcacttaag ttggtttgat ccatttgcct
     3241 ctggtcactt cgttcatgat cacggccgtc aagttagatt tttagctaaa gcagaggtat
     3301 ttaagttacc tggcattggt cgtgttttaa aaagtgcggg ccagataccg gttaaccgcc
     3361 aagattctga cgcagccgca ggatcactac gtattgcaat ggaggcagtc cgacgcggtg
     3421 aatgcattgt catatacccc gaaggcacct taactcgcga tccagattta tggccaatga
     3481 gtgccaagac cggtgcagcg cgcattgcct taacaactgg cgtgccaatt gttccggcag
     3541 cgcagtgggg gccacaagaa gttcttgcgc cgtatggaaa agtatttaaa atttttccgc
     3601 gcaaaactat gcacatctgg gccggaccac cagtagacct tgaagattta agaagccaac
     3661 caataaccgc ggccacattg cgtgaggcaa ctgatcgaat aatgcgtgac attacgaagc
     3721 tgctgggtga acaacgaggc ttaactccac ctgataatcc aatgccgcga aatgtcgtac
     3781 cacctcgcaa ggcagcatca gcatgaagcg agtagctgta atgggtgcag gttcctgggg
     3841 aactgtgttc tccgtgattt tggcggatgc aggttgtgaa gtatcgctct gggctaggtc
     3901 gacgcagtta gcagatgaga taaatagctc tcgagtaaat cgcttttatc atccaaattt
     3961 aaaattgccg gctgcagtaa cagccactgc aaatccagat gaggcgctag caaatagtga
     4021 attagtaatt ttggcggtac cggctcagac tttgcgcgag aatttaggcg cttgggctat
     4081 ctcgatacca actgaagcag ttatttgctc gctgattaaa ggtgttgaat taggaactgc
     4141 tgagcgcatg agtgaagtca tcgctgaagt tgccaaggtt gacattaatc gggttgcagt
     4201 aatttccgga cctaacttgg cacatgaaat tgctatgcgc gaacccgcag caacgaccgt
     4261 tgcttgcact gatgagcaat ccgctcgccg aattcaagat gccgtgacaa ctgattattt
     4321 ccgtccgtat tacacaactg atgtgatcgg cgttgaaatt gccggagccg ttaaaaacgt
     4381 aattgcactg gctaatggta tggcagttgg tcttggctat ggtgaaaatt cacaagcagc
     4441 aattatgacc cgagggctag cagaaatgac cagacttggt gtcgcacttg gcgcaaaccc
     4501 attaactttt gctggcctag ctggcgtcgg cgatttaatt gcaacctgtc agtcaccact
     4561 ttcgcgtaac cgaactattg gtgaacatat cggcaaaggg atgtcagttg aagaagcaca
     4621 acttgtgaca ttgacgactt ctgagggcgt taagagttgc cgatcgattt tggatttggc
     4681 aaataaacac aaagtagaaa tgccaattac cgaacaagtg gttaatgtca tacaccacgg
     4741 ccgaaatgca aatgatgtat tgcgcgcatt tatgtctaga tcaactcgtg ctgaagaagg
     4801 catcgagaaa atggaacacc attaggccat tagttaagcc gaatcatcgt atttagcaaa
     4861 aactctaata gcgttatacc tatggcaaat cggattcgcg tcgcagtcat ctttggcggc
     4921 cgaagtagcg aacacacaat ttcatgtatt tcagctggca gcgttttgcg ggcaatagat
     4981 cagaataaat atgaagttgt gccaatcgga attactcacg aaggtaaatg ggttttagag
     5041 tctgctgata gcgccagact ttctgtgacc gatggagttt tcccagctgt agacagttcg
     5101 cgttcgccaa tacttttcac accagatcca agtgcgacgc atttagttgt gcaatcggaa
     5161 gttcccgatg tgttagctca agttgatgtc gtatttcctg ttttacatgg cccatggggt
     5221 gaagatggca cgattcaagg tctgtttgaa ttagctggcg ttccttatgt gggttcaggt
     5281 gtttttgcat cggcagcagc aatggacaaa ggacacttga aagctttaat gaaagccgcc
     5341 ggtattccaa ttggtcctta cgaggttgtg acagatgcag a
//
LOCUS       DBNHVN010000215         5506 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1377643, whole genome shotgun sequence.
ACCESSION   DBNHVN010000215 DBNHVN010000000
VERSION     DBNHVN010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1377643"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /gene="atpA"
                     /locus_tag="AC16Y1_04045"
     CDS             <1..1187
                     /gene="atpA"
                     /locus_tag="AC16Y1_04045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020546686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR5831665.1"
                     /translation="LTGIKAIDAMTAIGRGQRQLIIGDRQTGKTAVAIDTILNQRENW
                     KTGDPKKQVKCIYVAIGQKGSTIASVKGALEEYGAMEYTTIVAAPASDPAGFKYLAPY
                     TGSAIGQHWMYKGEHVLIIFDDLSKQAEAYRAVSLLLRRPPGREAYPGDVFYLHSRLL
                     ERCAKLSDELGGGSMTGLPIIETKANDVSAYIPTNVISITDGQCFLESDLFNSGVRPA
                     INVGISVSRVGGSAQPKAMKKVAGRLRLDLAQYRELEAFAAFGSDLDAASKAQLERGA
                     RLVELLKQPQYQPQSMEREAVSVWAGTTGQLDEVPVEDIRRFDQDFLDYLDRNHKDIF
                     TTVRDTTDLSDDILARLEKAIGEFKKQFITTSGHALVNDAPVAALDESEIDPTQIVKV
                     RG"
     gene            1188..2111
                     /locus_tag="AC16Y1_04050"
     CDS             1188..2111
                     /locus_tag="AC16Y1_04050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIR5831666.1"
                     /translation="MGAQLRVYRRRIKSVQATKKITKAMELIASSRIVKAQQRVDASL
                     PYTLELMRAISAAVSHSSNSKHPLFTTVENRKRAAVILITADRGLAGAYSSAIIKEGE
                     ALNGLLKEEGVEAKHFLVGRKSVGFYKFRERPIAASWTGFTDQPTYENAKEIADTVLT
                     AFKEETQNGGVDEIHLVYTHFINMAVQEVRVRRILPVEVVEVSEEEAAVKLETKAFPL
                     YDFEPSAEAVLDALLPRYIENVVYTSLLLAAASEHAARRRAMKSASDNAEELIRTLAR
                     QANQARQAEITQEISEIVGGADALSSASAGS"
     gene            2118..3539
                     /gene="atpD"
                     /locus_tag="AC16Y1_04055"
     CDS             2118..3539
                     /gene="atpD"
                     /locus_tag="AC16Y1_04055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR5831667.1"
                     /translation="MTATTTSVGRVARVTGPVVDVEFPVESMPGLYNALHVDVDFGSE
                     KRTLTLEVAQHIGDNMIRAISMQPTDGLVRGAQVRDTGDSITVPVGDVTKSHVWNTLG
                     KPLDVEESSLQIKERWGIHRQAPSFDQLESKTEVFWTGIKVIDLLTPYVKGGKIGLFG
                     GAGVGKTVLIQEMITRVAKNFGGVSVFAGVGERTREGNDLFLEMTESGVINDTALVFG
                     QMDEPPGTRLRVALSALTMAEYFRDVQKQDVLLFIDNIFRFTQAGSEVSTLLGRMPSA
                     VGYQPTLADEMGVLQERITSTRGHSITSLQAIYVPADDLTDPAPATTFAHLDATTVLS
                     RPISEKGIYPAVDPLDSTSRILDARYVGEEHYAVAARVKEILQRNKELQDIIAILGID
                     ELSEEDKILVNRARRIERFLSQNMFVAEAFTGQKGSFVPVEETVASFKKLTEGEYDHL
                     PEQAFFMCGGIDDVEANAKKLKG"
     gene            3543..3803
                     /locus_tag="AC16Y1_04060"
     CDS             3543..3803
                     /locus_tag="AC16Y1_04060"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HIR5831668.1"
                     /translation="MSALNVAVVSVGQNIWSGEAKSLVAKTTEGEIGILAGHEPVLSL
                     LVDGVVRVEGVAGEKIEVSITGGFLAVDNNSVRVLAEEAQVL"
     gene            complement(3848..4570)
                     /locus_tag="AC16Y1_04065"
     CDS             complement(3848..4570)
                     /locus_tag="AC16Y1_04065"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR5831669.1"
                     /translation="MSIARTALLSPIEPAKATDQVIQRISVAISSGVFKPGDQLPVEA
                     ELAAQLNVAQMTLRQALAILRDLGIIETVRGRNGGSFVTTRPLALGGVFVGPVPTMAE
                     LQDLTDYRMALENEAAALAAIRADRIDLGRLNKSLETCLTSPNEDDHAVEDNSFHICI
                     AETSHSYRLVTAITQLQHEMTNFYQRLTRPNEAIFPHKDEHINIVRAIESGDADAAWE
                     ASNVHLYSTYLFLESLIKKITE"
     gene            complement(4697..>5506)
                     /locus_tag="AC16Y1_04070"
     CDS             complement(4697..>5506)
                     /locus_tag="AC16Y1_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR5831670.1"
                     /translation="DTDTRDSRYSNFGACTDLYAPGQSIFSAYPKNSSNVFGDNFYAR
                     MSGTSMAAPFVSGVAANLLMAEPTLTPSTLSTKLLTFTNPVITTDKANDPNRLIYQPE
                     TGFVTPVNNNSSVVDKNAANPVVETTPIALAPAPTPVVEEPTTPPAPPPAPAPVAAPA
                     PAAAPAAAPAASGAIAQTVGARRVINVSVSAQAGSTTSIQIQSRKNQRVAYKTAAGKT
                     RYRTVKVTFWRTVSTTPTVASAKIRVRSAGTYRILVNTPAGPLEGAPFRVR"
BASE COUNT         1450 a         1233 c         1354 g         1469 t
ORIGIN      
        1 tgcttaccgg catcaaagcg attgacgcaa tgactgcgat tggccgtggt caacgtcagt
       61 tgatcattgg tgaccgccag actggtaaaa ctgcagttgc aatcgacact attttgaatc
      121 agcgcgaaaa ctggaaaact ggcgatccaa agaagcaagt taagtgtatt tacgttgcga
      181 ttggtcaaaa gggttcaact atcgcttcag taaaaggtgc acttgaagag tacggtgcga
      241 tggaatacac cacaatcgtt gcggcgcctg catctgaccc agcaggcttc aagtacttag
      301 ctccgtacac tggttcagcc attggccagc actggatgta caaaggcgaa catgtattaa
      361 ttatttttga tgatctttcc aagcaagctg aggcttaccg tgcagtatcg ctattactac
      421 ggcgcccgcc aggtcgtgaa gcttacccag gcgatgtttt ctacttacac tctcgcttac
      481 ttgagcgttg tgcaaagtta tctgatgaac ttggtggcgg ttcaatgacc ggtttgccga
      541 ttattgaaac aaaagcaaat gacgtatctg cttatatccc aactaacgta atttcgatta
      601 ccgatggcca gtgcttcctc gagtctgatc tgttcaactc gggtgttcgc ccagcgatca
      661 acgtgggtat ttcggtatcc cgtgtaggtg gctcagcaca accaaaggca atgaagaaag
      721 ttgccggtcg tttacgtcta gatcttgcgc agtatcgcga actcgaagca ttcgctgctt
      781 ttggttcaga tcttgatgca gcctcaaaag cacagctaga acgtggtgct cggctagttg
      841 aactgctaaa gcaaccgcag taccagccgc aatctatgga acgtgaagct gtctcagttt
      901 gggcgggtac aactggccag ctagatgagg tgccggttga agatatccgt cgttttgatc
      961 aagatttcct tgattatcta gatcgcaatc ataaggacat ctttacaact gttcgtgata
     1021 cgactgattt gtctgatgac attttggctc gacttgaaaa agcaatcggc gaattcaaga
     1081 agcaattcat cactacctcc ggacatgcat tggtaaacga tgctccagtt gccgctcttg
     1141 atgaatcaga aatcgaccca actcagattg tgaaagtacg gggctaaatg ggcgcacagt
     1201 taagggttta ccgacgacgg attaagtccg ttcaggcaac caagaagatc acgaaggcaa
     1261 tggaactcat tgcgtcttcg cgtattgtca aagcgcagca gcgcgttgat gcttcgttgc
     1321 cgtacaccct tgaattgatg cgggctatct cagctgcagt ttcacacagt agtaacagca
     1381 agcacccact ttttactacg gtagaaaacc gtaagcgagc tgcggttatt ttgattactg
     1441 cagaccgtgg tctagctggc gcttattcat cagcgatcat caaagaaggc gaagcgctaa
     1501 atggcctact caaagaagaa ggcgtggaag caaagcactt cttagtgggg cgtaagtcag
     1561 ttggtttcta caaattccgc gaacgtccga ttgcggcctc ttggactggt ttcactgatc
     1621 agccaacata cgagaatgca aaagaaattg cggacaccgt tttaactgca ttcaaggaag
     1681 aaacgcaaaa tggtggggta gatgaaattc atttggttta cacccacttc ataaacatgg
     1741 ctgtccaaga agtacgggtg cgccgaatac ttccagttga agttgttgaa gtttctgagg
     1801 aagaagccgc agtcaaactt gagaccaagg ctttcccgct ttacgatttc gagcccagcg
     1861 cagaagctgt actcgatgca ctactgccgc gttatatcga aaacgtggtt tacacatcac
     1921 ttttgttagc tgccgccagt gagcacgccg ctcgtcgtcg tgccatgaaa tctgcatctg
     1981 acaatgccga agaacttatt cgaacacttg cccgacaagc taaccaagct cgtcaggctg
     2041 aaatcaccca ggagattagt gaaatcgtcg gtggcgccga tgcactttcc tccgcatcag
     2101 cagggagcta aacaatcatg accgcaacca caacctctgt cggccgcgta gcacgcgtta
     2161 ccggtcctgt tgtagacgtg gagtttccag tcgaatcaat gccaggtctt tacaacgcat
     2221 tgcatgttga cgtggacttc ggtagcgaaa aacgcacact aactcttgaa gttgcccagc
     2281 acatcggcga caacatgatt cgtgctattt cgatgcagcc aactgacggt cttgtccgtg
     2341 gcgcacaggt gcgcgacact ggtgattcga tcacggtgcc tgttggtgat gtaactaaga
     2401 gtcacgtgtg gaacactcta ggtaagccac tagatgttga agaatcatca cttcaaatca
     2461 aagagcgttg gggcattcac cgccaagctc catcatttga tcaacttgaa tctaagacgg
     2521 aagttttctg gactggtatc aaagttatcg atctactaac tccatatgta aaaggtggaa
     2581 agatcggtct attcggtggt gcgggtgttg gtaaaaccgt tctgattcaa gaaatgatca
     2641 cccgtgtagc taagaacttt ggtggtgttt ctgtattcgc aggtgtgggc gagcgtactc
     2701 gtgaaggtaa tgacttattc cttgaaatga ctgaatcagg tgttatcaat gacaccgcac
     2761 tggtattcgg tcagatggac gaaccaccag gcacccgtct tcgcgtggca ctttctgcgc
     2821 taactatggc tgaatatttc cgcgatgtac aaaagcaaga cgtactgctt ttcattgaca
     2881 atatcttccg tttcacgcaa gcaggctctg aggtatcaac tttgcttggt cgtatgccat
     2941 cagcagtggg ttaccagcca acacttgctg acgaaatggg tgtattgcaa gagcgcatta
     3001 cctcgacacg cggtcactca attacttcgt tgcaggcaat ttatgtacct gcagacgact
     3061 taactgaccc cgcaccggca actactttcg cgcacttaga tgcaacaaca gtgttgtctc
     3121 gtccaatttc ggaaaaaggt atttatccag cggtagatcc acttgactca acatctcgca
     3181 tcttggatgc tcgctatgtt ggtgaagaac actatgctgt tgccgctcgc gttaaagaaa
     3241 tcttgcagcg aaataaagaa cttcaagata tcatcgcaat tcttggtatc gatgaacttt
     3301 ctgaagaaga caagattctg gtaaaccgag cccgtcgtat tgagcgcttc ctatctcaga
     3361 atatgtttgt ggctgaagcg ttcactggcc aaaagggttc atttgtgcca gttgaagaaa
     3421 ctgtcgcatc attcaagaag ctaactgaag gcgagtatga ccaccttcca gaacaggcct
     3481 tcttcatgtg tggcggtatc gatgatgttg aagctaacgc caagaaactt aagggctaat
     3541 taatgtcagc attaaacgtg gcagttgttt cggttggtca gaatatttgg tcaggcgaag
     3601 caaagtcact agttgccaaa accactgaag gcgaaatcgg tatcttggct gggcacgaac
     3661 ctgtactttc cttgctagtt gatggggtag tgcgagttga aggcgtagct ggagaaaaga
     3721 tcgaagtttc tattaccggt ggtttcttgg cagttgataa caatagcgtt cgagtactag
     3781 ctgaagaagc tcaagttctg taatcaaaaa acctaaaagc ccgctccatg tgagcgggtt
     3841 ttttatttta ctcagtaatt tttttaatta ggctttcgag aaatagataa gttgaataca
     3901 aatgcacatt tgaagcttcc caagcagcat ccgcgtcacc tgattcgatt gctcgcacaa
     3961 tgtttatgtg ctcatcttta tgcgggaaaa ttgcttcatt tggcctggtg aggcgttgat
     4021 aaaaattggt catctcgtgc tgcagttggg tgattgctgt aaccaagcgg taggaatgac
     4081 tggtctcggc aatgcaaata tgaaaactgt tatcttcaac tgcatggtca tcttcgttgg
     4141 gactagtcaa acaagtttct aaacttttat tcaatcgacc aagatcaatg cgatctgctc
     4201 tgatcgcagc taatgctgcc gcttcgtttt caagtgccat gcggtaatca gttagatctt
     4261 gtaattcagc catggttgga actggaccaa caaacactcc gccaagcgcg agtggacgtg
     4321 ttgtaacaaa tgagccaccg tttcggccgc ggacagtttc aataatgcct aaatcgcgca
     4381 aaattgctaa ggcctggcgc aatgtcattt gcgccacgtt tagttgtgca gcaagttcgg
     4441 cttcgacggg aagttgatcg ccaggtttaa aaacaccaga acttattgct actgatatcc
     4501 gctggataac ttgatcagtt gccttggctg gctcgatcgg gctgagcaag gctgtgcgtg
     4561 caatagacat cccgagtact ccttaggtaa gaatttcccc ttggtcttat tgtgtcgaat
     4621 gagtctgaca aaataccgaa aattaatgtc aaattatcta tgttgcgaaa aagtgataac
     4681 tatgtgaaac atttcgctag cgaactctaa atggtgcgcc ttcaagaggt ccagctggcg
     4741 tattaaccag aattcgataa gttccagcag aacgtacccg aatttttgct gaggcaacag
     4801 ttggagtagt tgaaacagtt cgccagaaag tcaccttaac tgtgcgatat ctggttttgc
     4861 cggcagcagt tttataagca acacgctggt tcttgcgcga ctgaatttga attgaagtag
     4921 tgctacctgc ttgggcactt accgaaacgt taatgactcg acgcgcgcca actgtttgag
     4981 cgattgcacc acttgcagct ggtgccgctg ctggtgcagc agcgggagca ggggcagcga
     5041 ccggtgcagg ggccggtgga ggtgcaggtg gggttgttgg ttcttcaaca actggcgttg
     5101 gcgccggagc taatgcaatc ggagtagttt cgactactgg atttgcagca tttttatcaa
     5161 ccaccgatga attgttgttt accggagtaa caaatcccgt ttctggttga tagatgagtc
     5221 gatttggatc atttgctttg tccgtagtga tcaccggatt agtaaaagtt aataatttgg
     5281 tacttaaggt acttggtgtc aaagttggct cagccattag caggtttgcg gccacaccac
     5341 taacaaaggg cgctgccatt gaagtgcctg acattcttgc ataaaagttg tcaccgaaaa
     5401 cgttggaaga attttttggg tatgccgaaa agatactttg accgggtgca taaagatcag
     5461 tacatgcacc aaaatttgag tacctactgt ctcgcgtgtc agtatc
//
LOCUS       DBNHVN010000216         2797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1005555, whole genome shotgun sequence.
ACCESSION   DBNHVN010000216 DBNHVN010000000
VERSION     DBNHVN010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1005555"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="AC16Y1_04075"
     CDS             <1..798
                     /locus_tag="AC16Y1_04075"
                     /inference="COORDINATES: protein motif:HMM:NF021214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HIR5831671.1"
                     /translation="IGRTISFLIAATLLIWTSSGGISKYAMISFSAHMIQHMTLSMIA
                     PIFLVLSAPITLLLRSLPTKTEVGTKNAREWILGLLNSGYAKVITHPIMVLVIFTFGL
                     YGLYFTPLFGDLMRSHSGHVFMEIHFLFSGFLFAFMTVGIDPAPRQIPHWSKLMMVLV
                     ALGLHTFFALAIMQTTVPIGIDWYAQVQPPWIYDLLRENYEGGGIAWAIGEIPTLILA
                     IIVAVQWARSDSKLAVRKDRAAQRDGDAELKAYNERLAKLNNFDQQN"
     gene            complement(795..1574)
                     /locus_tag="AC16Y1_04080"
     CDS             complement(795..1574)
                     /locus_tag="AC16Y1_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HIR5831672.1"
                     /translation="MTKVALLQVSTDENETVSDRITRVTQLTKEWATKADFVVLPELW
                     HVGAFSSNLFEEYALPTENDLIKSFCQIAKDSNCWIHFGTFIEKHEKGISNTAVTINN
                     LGEIVSQYRKIHLFGFDQGEATMLSRGDKPAVISNTPLGVTGVSTCYDLRFPELFRAQ
                     VDQGAQTLLIASGWPTPRIEHWRVLNTARAIENQAWVIACNQVGKNADYILGGHSMVI
                     SPKGEIVGELGTTEDVLVAQIDLDLVNAWRDTFPVLKDRVL"
     gene            complement(1594..2079)
                     /locus_tag="AC16Y1_04085"
     CDS             complement(1594..2079)
                     /locus_tag="AC16Y1_04085"
                     /inference="COORDINATES: protein motif:HMM:NF022819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3180 domain-containing protein"
                     /protein_id="HIR5831673.1"
                     /translation="MLTPTRITYLIYLAFMTTGIGFIVASVWPRIFGNAFATPWLTAI
                     TMWLLATALFVWTILARKKIKPEKGKPRLDPILAARSAALAMSASRVGALALGFYLGV
                     FIDNLFFSDSAASNERAIICALTSLASLVTVVIGLWLEHICRITQPPTDANSSAVPTS
                     G"
     gene            complement(2079..2582)
                     /gene="folK"
                     /locus_tag="AC16Y1_04090"
     CDS             complement(2079..2582)
                     /gene="folK"
                     /locus_tag="AC16Y1_04090"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR5831674.1"
                     /translation="MAVCYLSLGSNLGDRLAHLTNAVSALKSQPKIKVLKVSSVYQTR
                     PVGGPDQDDFLNAVIKIDTSLTSTELLEITQGIENSANRTREVHWGPRTLDIDILSYA
                     DVVSADEKLTLPHPRISQRAFVLIPWNEIDPTAQIPGLGEVSKLVNQVSSEGVQLNSD
                     MKLPGVS"
     gene            complement(2582..>2797)
                     /locus_tag="AC16Y1_04095"
     CDS             complement(2582..>2797)
                     /locus_tag="AC16Y1_04095"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010079669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR5831675.1"
                     /translation="KNTVNYAEVAKLVHQFITGTPVDLIETLADNIADQLAKLPLVKA
                     VEVTVNKPNAPIDLPFENVSVTVYRGA"
BASE COUNT          785 a          588 c          578 g          846 t
ORIGIN      
        1 attggtcgca ctatctcatt tttaattgcg gctactttac taatctggac tagctctggt
       61 ggcatttcaa agtacgcaat gatttcattc agcgcgcaca tgattcaaca catgacgctt
      121 tcaatgattg cgccaatatt tttagtcttg agtgcaccaa ttactttgtt acttcggtca
      181 ttaccaacaa aaaccgaagt tggcaccaag aatgcccgag aatggatcct tgggttacta
      241 aacagtgggt acgccaaggt aataactcat ccaattatgg ttttagttat ttttactttt
      301 ggtttatacg gactgtactt cactccattg tttggcgact taatgcgcag tcatagcggc
      361 cacgtattta tggaaatcca tttcttattt tctggattct tgtttgcatt tatgactgtt
      421 gggattgatc cagcaccacg tcagattcct cactggagta agttgatgat ggttttagtc
      481 gcacttgggt tacatacctt cttcgcttta gcgattatgc aaacaacggt tccgattggc
      541 attgattggt atgcccaagt tcagccaccg tggatttatg atttgctgcg cgagaactac
      601 gaaggtggtg gtatcgcctg ggcaattggc gaaatcccga ctttaatatt ggcaattatt
      661 gtggccgtgc aatgggccag aagtgattcg aaattagcgg ttagaaaaga tcgagcagct
      721 caacgcgatg gtgacgcaga acttaaggct tataacgaac ggttagcaaa attaaataat
      781 ttcgaccaac agaattaaag aactcgatcc tttaatactg gaaatgtgtc gcgccacgca
      841 tttactaaat ccagatcaat ttgcgcgact aatacatcct cagtggttcc tagttcacca
      901 actatttcac ccttagggct gatcaccata gagtgaccac caagaatgta atctgcattt
      961 tttccaactt gattacatgc aataacccaa gcttggtttt caattgcgcg ggcggtattc
     1021 aaaactcgcc agtgttcaat acgcggggta ggccaacctg atgcaattaa aagcgtttgg
     1081 gcgccttgat caacttgtgc tctaaataat tctggaaaac gtaggtcgta acaagttgat
     1141 actccggtca cacctaaggg tgtattgctg ataactgccg gtttatcgcc acggcttaac
     1201 atagttgctt caccttgatc aaaaccaaat aaatgtattt ttcggtactg acttacaatc
     1261 tcaccaagat tgttgatagt gaccgccgta tttgaaatac ctttttcatg cttttcaatg
     1321 aaagttccaa aatgaatcca gcaattactg tctttcgcaa tttggcaaaa actttttatt
     1381 aagtcatttt cagttggtaa tgcgtattct tcaaacaaat ttgaactaaa tgctccaaca
     1441 tgccaaagct caggcaaaac tacaaaatct gctttagtag cccattcttt agttagctgt
     1501 gtaactcgag ttattcgatc acttactgtt tcgttttcat ctgtacttac ttgcagcagc
     1561 gcaactttgg tcatgactaa agactattgt gctttaacca gaagttggta cggcagaaga
     1621 atttgcatca gttggaggct gcgtaatgcg acaaatgtgt tcaagccata atccaattac
     1681 taccgttacc aagctagcta atgaggtgag tgcgcaaata attgctcgtt cgttgcttgc
     1741 agctgaatca gaaaaaaata agttgtcgat aaagacacct aagtagaaac ccagtgccaa
     1801 tgcgccaact cgagaggcag acattgcaag tgctgcgctg cgggcggcca aaattggatc
     1861 aagtcgaggt ttgccctttt ctggttttat tttttttcgc gcgagtattg tccaaacaaa
     1921 taaggctgtc gctaataacc acatcgtgat tgcggttagc cagggagttg caaatgcatt
     1981 gccaaatatc cgaggccaaa cacttgccac aataaacccg atgccagttg tcataaacgc
     2041 taaataaatt aagtacgtta tgcgcgttgg ggtaagcatt agctaactcc tggcaatttc
     2101 atatcactat ttaactgcac accttcggat gaaacttgat ttaccagctt tgaaacttcg
     2161 ccaagtcctg gaatctgagc ggtcggatcg atttcattcc acgggattaa cacaaatgcg
     2221 cgctgcgaaa ttcttggatg cggaagcgtt aatttttcat ctgcacttac aacatccgca
     2281 tagctgagaa tgtctatgtc tagagtgcgc ggtccccagt gaacttcacg tgtacgattg
     2341 gccgaatttt caataccttg ggtaatttca agtaattctg tcgaagtcaa agatgtatca
     2401 atttttataa cagcattcaa aaaatcatct tggtccggac caccaactgg tcgagtttgg
     2461 taaacagatg aaacttttaa aactttgatc tttggctgac tttttaacgc actcacagca
     2521 tttgttagat gtgcaagtcg atcacccaga tttgagccaa gtgataaata acagactgcc
     2581 attaagcacc gcggtaaaca gtcacagaaa cgttttcaaa aggtaaatcg attggtgcat
     2641 ttggcttatt gaccgttact tctacggctt taactagtgg caatttagct aattggtcgg
     2701 cgatgttgtc agctaaggtc tcaattaaat caactggtgt tccagttatg aattggtgga
     2761 ctaacttagc aacttctgcg tagttaacgg tgttctt
//
LOCUS       DBNHVN010000217         3882 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_113060, whole genome shotgun sequence.
ACCESSION   DBNHVN010000217 DBNHVN010000000
VERSION     DBNHVN010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_113060"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(50..198)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01482"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The adenosylcobalamin (AdoCbl) riboswitch is found
                     in the 5'-untranslated regions of vitamin B12 (Cobalamin)
                     related genes in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF01482"
     gene            complement(235..1242)
                     /locus_tag="AC16Y1_04100"
     CDS             complement(235..1242)
                     /locus_tag="AC16Y1_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HIR5831676.1"
                     /translation="MEFRYLGNSGLLVSEISYGNWITHASQVTEDTAIACVKAALDAG
                     ISTFDTADVYANTAAEEVLSKALKGQRRESLEIFTKVYWPTGPRGHNDCSLSRKHIME
                     SCNGSLKRLGTDYIDLYQAHRFDVTTPLEETLVAFDDLIRQGKVLYIGFSEWNASQIT
                     QALKIAKELGLTKFISSQPQYSMLWRVIESEVDPVCRENGISHIVWSPIAQGALTGKY
                     LPGQPPPKDSRATSSEGGSVTDRFLKDDILSAVQNLKPIAQQVNLTMAQLAVAWVLQN
                     KNVASAIIGASKPEQITEAAKASGVKLPAEVMSAIDKALAGVVATDPRLTVSPDDRPV
                     W"
     gene            complement(1255..1812)
                     /locus_tag="AC16Y1_04105"
     CDS             complement(1255..1812)
                     /locus_tag="AC16Y1_04105"
                     /inference="COORDINATES: protein motif:HMM:NF022686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3043 domain-containing protein"
                     /protein_id="HIR5831677.1"
                     /translation="MSTSDNSSTGKGRPTPKRKDVEAQRQQGFSAKRGTKEANRAARE
                     RAREMRAQARAGLAAGDPRYFPRRDAGPVKAKVRDYIDSRRTVGEVFVVVAFLVLALG
                     LVNNAQLQAVVVFAWTAVLLLVLIDTTIVGLLLARTLSKEFPDKAMRKGAISYGVLRA
                     LQIRKFRIPPPRVRPGGKPITPKNK"
     gene            1838..2950
                     /locus_tag="AC16Y1_04110"
     CDS             1838..2950
                     /locus_tag="AC16Y1_04110"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase family protein"
                     /protein_id="HIR5831678.1"
                     /translation="MRILVSPDCFTGTLTSAQAATSIVQGWQEIHPEDIVDVAALSDG
                     GPGFVSAIATTLGATEVPLVVTGPLGDKVLAQFALLGNRAWIESAQACGLHLIKKSAR
                     DPQFTTTYGVGELILAAIESGAPEITIGLGGSGTNDGGAGMLAALGATADAPLAQGAL
                     SLANVTSIDLSKALLATSGVQLICASDVDNPLLGLRGATNVFAKQKGATEETIMRLEG
                     ALENFAQVCGLRADGKNPAIALGAGAAGGLGYGLLLLGATRVAGIETVMQIVSLDEKI
                     ANADLVITGEGCLDDQSLQGKVAIGVAAHCVAASKPCVVIAGEVRLGKREYLSAGIDA
                     AYSMSQIAGKEHSMANAPEVIKQVASRVAKTWGRRA"
     gene            3060..3407
                     /locus_tag="AC16Y1_04115"
     CDS             3060..3407
                     /locus_tag="AC16Y1_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HIR5831679.1"
                     /translation="MTTEATQSSSVVITDVAANKVRALLDQEGRDDLNLRIAVQPGGC
                     SGLRYQLFFDDRNLDGDVRQEISGVGVVVDRMSVPYLGGAVIDFVDTIEKQGFTIDNP
                     NATGSCACGDSFH"
     gene            3474..>3882
                     /locus_tag="AC16Y1_04120"
     CDS             3474..>3882
                     /locus_tag="AC16Y1_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006136603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR5831680.1"
                     /translation="MRIGITGSVATDHLMTFPGKFNDSLMADQLHNVSLSFLVDDLQI
                     RRGGVAANICFGMGTLGQHPYLIAAVGHDFDSYRDWLQNVGVKCDYVHTSQTAHTARF
                     MCTTDQEQNQIASFYPGAMSESSQIDVESISANV"
BASE COUNT         1009 a          876 c          957 g         1040 t
ORIGIN      
        1 ctcctaatgt ggccgaagaa aaactcggcg aacacaaaag gagtgcatcg acgaactctt
       61 cctcgagagt ttttaacgaa tgcagtaaca ggcgacctgg cttttggttt ccaaaaactt
      121 gggttaacca aattacagtt gcgggacagc gccggaatca caccggactt cgctggtatt
      181 gcagttaggt aaacgtagca caatgctcga gtgaaaacgg gtaagggtcg aaaattacca
      241 aactggtcga tcatcaggtg acacagtcag ccgaggatca gtggcaacaa ctcctgcaag
      301 tgccttatca atagcggaca ttacctcagc aggtaactta actccgcttg ctttcgcagc
      361 ttctgtaatt tgctctggct tcgaagcacc aataattgca cttgctacat tcttattctg
      421 caaaacccaa gccacagcta attgcgccat ggtcagatta acttgctggg caattggctt
      481 taaattttgc actgccgaca aaatatcatc ctttaagaat cggtcagtta ctgaaccacc
      541 ttcgctggat gtggcacgac tatctttagg tggcggttga ccaggtaagt atttaccggt
      601 tagcgcacct tgggcaatag gtgaccacac tatgtgactt attccatttt cgcgacaaac
      661 tggatcaact tccgattcaa ttacccgcca caacattgaa tactgcggtt ggctcgaaat
      721 aaatttcgtt aagccaagtt ctttggcaat cttgagtgct tgggtaatct ggcttgcgtt
      781 ccattctgaa aaaccaatgt aaagaacttt tccttgtcgg attaagtcgt caaatgcaac
      841 aagtgtctct tcaagtggag tcgtgacatc aaatctgtgt gcctggtaaa ggtcaatgta
      901 gtcagtgcca agacgcttaa gcgaaccatt gcaggattcc ataatgtgct tgcgggagag
      961 gctgcaatcg ttatgcccgc gtgggccagt aggccaataa acttttgtaa atatttccag
     1021 tgattcgcgt cgctgaccct ttagggcttt acttaaaact tcttcggcag ccgtgttggc
     1081 ataaacatct gccgtatcaa aagttgaaat ccctgcatct agggcagctt tcacgcatgc
     1141 aattgcagta tcttcggtta cttgcgatgc atgagtaatc cagttgccat atgaaatttc
     1201 tgaaacgagc aacccactgt ttccaaggta acgaaattcc atattggctc cttgttactt
     1261 attttttgga gtaatcggtt tgccaccagg gcgaacgcgg ggtggcggaa ttctgaattt
     1321 acgtatttga agtgcgcgca atacgccgta tgaaatcgcg cctttacgca ttgctttatc
     1381 tggaaattct ttactcaacg ttcttgctag gagtaagcca acaattgtgg tgtcaattaa
     1441 aactagaagt aaaactgcag tccaagcaaa cacaactacc gcttgtagtt gagcgttatt
     1501 aacaaggcca agtgctagaa ctaaaaatgc tacgacgacg aatacttcac caacggttcg
     1561 acgggagtct atataatcac gaacttttgc cttgacgggt ccagcgtcgc gacgcgggaa
     1621 gtatcttggg tcaccagcag ccaggccagc acgagcttgt gcgcgcattt cgcgtgctcg
     1681 ctcgcgagca gcacgatttg cctctttggt tccacgtttg gcactgaagc cttgctgccg
     1741 ttgcgcttct acgtctttgc gttttggcgt cggtcggcct ttgccggtac ttgaattgtc
     1801 actggtactc ataatgatta atgctaacct gcccctcatg aggattttgg tcagcccaga
     1861 ttgctttact ggaacgctaa cctcagcaca ggcagcaact tcaatagttc agggctggca
     1921 agaaattcac cccgaagata ttgtcgatgt agctgcgcta tctgatggcg gaccaggatt
     1981 cgtttctgcg attgcaacaa ctttaggcgc aaccgaagtc ccgcttgtag tgaccggacc
     2041 gctaggtgac aaagtacttg cccaatttgc tctgcttgga aaccgtgcct ggattgaatc
     2101 agcacaggct tgcggtttgc acttaattaa aaaatctgcc agagatcctc aattcacaac
     2161 tacttatggt gttggcgaat taattttggc agcgattgaa tcgggagctc ctgaaataac
     2221 tattggcctt ggtggtagcg gcacaaacga tggtggcgct ggtatgttgg ctgcgcttgg
     2281 cgcgactgct gatgcaccac tagcacaagg cgcactttcg ttagccaacg taacctcaat
     2341 cgatttatca aaagcgcttt tggcaacttc aggagttcag ttaatttgtg ccagtgatgt
     2401 ggataatcca ttacttggac tacgtggggc gacaaatgtt tttgctaaac aaaaaggtgc
     2461 gactgaagaa acaattatgc gactagaggg cgctttagaa aattttgctc aggtttgcgg
     2521 attgcgtgca gatggaaaaa atccagcaat agctttaggc gccggtgcag caggtggctt
     2581 gggttatgga ctactacttc tcggcgcgac ccgagttgct gggattgaaa cggtcatgca
     2641 aattgtttcg ctggatgaaa aaattgcgaa tgctgattta gtcataaccg gcgaaggctg
     2701 ccttgatgat caaagtctgc aagggaaagt tgctattggc gtggccgccc actgtgtggc
     2761 agcgagtaag ccttgtgtgg ttattgctgg tgaggtccgc ctaggtaaac gtgagtacct
     2821 ttcagctgga atagatgctg cttactcaat gtcgcagatt gcgggcaagg aacactcaat
     2881 ggcaaatgcc cccgaggtca taaagcaggt tgcaagccgg gtagccaaaa cctggggtcg
     2941 ccgggcctag gccgtcggct ggcgaatctg cgcttcgggg aaaatcaagt taaaatcaag
     3001 ggaataattg ttggaccaat aaggttgcaa taagtagaaa atttccgagc aggagtgtca
     3061 tgacaacgga agcaactcaa agttcgtcag tagtaattac agacgttgcc gctaacaaag
     3121 ttcgcgcatt attagaccaa gaaggtcgcg atgacttaaa tcttcgaatt gcagttcagc
     3181 cgggtggttg ctctggactt cgttaccaac ttttctttga tgaccgcaat ttagacggcg
     3241 atgttcgcca agaaatcagt ggcgtgggcg tagttgtgga ccgcatgagc gttccttacc
     3301 ttggtggggc agtaatcgat tttgtcgaca caatcgaaaa gcaaggcttc actattgata
     3361 atccgaatgc cactggctca tgtgcctgtg gagattcttt ccactaaatc gaattagaaa
     3421 aaatcccgta gtcgcctgcc gactgcggga ttctttcgtt agagtcaata cttgtgcgta
     3481 taggtatcac aggttctgta gcaactgacc acttaatgac atttccaggc aagttcaacg
     3541 actcgttgat ggccgatcaa ttacacaatg tgtcactttc atttttagtc gatgacttgc
     3601 aaattcgccg tggtggagta gccgccaata tctgctttgg aatgggcact ttaggacaac
     3661 atccatattt aatagcagct gttgggcacg attttgatag ttaccgtgac tggttgcaaa
     3721 atgtgggcgt caaatgcgac tacgtgcata cttctcaaac tgcacatact gcgagattta
     3781 tgtgcacgac agatcaagag caaaatcaga ttgcctcgtt ctacccaggt gcgatgtcgg
     3841 aatctagcca aatcgacgtc gaaagcatat ctgcgaatgt gg
//
LOCUS       DBNHVN010000218         2270 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_931127, whole genome shotgun sequence.
ACCESSION   DBNHVN010000218 DBNHVN010000000
VERSION     DBNHVN010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_931127"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="AC16Y1_04125"
     CDS             complement(<1..307)
                     /locus_tag="AC16Y1_04125"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009759768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR5831681.1"
                     /translation="MNSDIDIARAAKTKPIGEIAAGIGIPADAVFNFGPTKAKVSMEF
                     IDSLKNKKKGKLILVTAMTPTPAGEGKTTTTVGLGDGLNAIGKKAIICLREPSLGPCF
                     "
     gene            complement(304..1743)
                     /locus_tag="AC16Y1_04130"
     CDS             complement(304..1743)
                     /locus_tag="AC16Y1_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR5831682.1"
                     /translation="MFQNKSPRFEILSQDAMAALDKGWKRIVSEIGIEFLSDEALDLF
                     RNAGQKVEGNNVKFDPEFILAQVAKAPKEFDVQARNPKNNVHIGGDSMVFAGNYGSPF
                     VLEDGVRRDAKLRDYENLLKLTQSFSDLDVASSVICEPEDTHLDTRHLDMTYRLQTLT
                     DKVYMGNVVSAQNARDVIKMSEILFGGREAIEKTPTTISLINCNSPLRWDDRMLESQF
                     EYTRANQAVILTPFLLMGAMSPVTIPASLVQQIAEALSGVALSQLIRPGSPVIFGSFL
                     SNIDMQSGSPMFGTPESAIGLLCTGQIARHFGLPFRGGGGLNASQTVDAQAGYQTLMT
                     MHATFLAGTNFVLHTAGWLEGGLVASYEKFVMDMEILQMMQVEFTPFEIDEASLGFDA
                     HIEAGHGGHFLGTMHTMERFRDCFYRPFLSSSDNFDRWSRNGSKDTAERANEYYKKML
                     DEYEQPALDTAIKEELDEYVAKRSKELTA"
     gene            1961..>2270
                     /locus_tag="AC16Y1_04135"
     CDS             1961..>2270
                     /locus_tag="AC16Y1_04135"
                     /inference="COORDINATES: protein motif:HMM:NF020897.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5831683.1"
                     /translation="MEDKTVTGTQSIDRACDLLIRVIDSEKPQTLSELVSATGLAKGT
                     TSRILNALERAGLLGRSNLGGFTPGPVLNQFAVAGGAYTALVKSVTPAMESIAAQTKE
                     T"
BASE COUNT          632 a          513 c          494 g          631 t
ORIGIN      
        1 caaaacatgg tccaagtgaa ggttcacgta aacaaataat tgctttcttg ccaattgcat
       61 tcaagccatc gccaagacca acagttgtag ttgttttacc ttcaccagcg ggagtcggtg
      121 tcattgcagt aacaagaatt aatttgcctt ttttcttgtt ttttaacgaa tcaataaact
      181 ccattgaaac ttttgctttt gttggaccga agttgaaaac tgcatccgcc ggaattccaa
      241 taccggccgc tatctcacca attggttttg tttttgcggc tcttgcgata tcgatatctg
      301 agttcatgca gttagctcct ttgatctttt ggcaacgtac tcatctagtt cttctttaat
      361 ggctgtatca agtgctggtt gttcgtactc atccaacatc ttcttgtaat actcgttagc
      421 tcgctcagca gtgtcttttg agccgttacg tgaccagcga tcaaagttgt cgcttgatga
      481 aaggaatgga cggtagaagc aatcacggaa acgttccatt gtgtgcatag ttcctaagaa
      541 atgaccacca tgtcctgctt ctatgtgagc atcaaaacca agtgaagctt catcgatttc
      601 aaatggagtg aattccacct gcatcatctg caaaatttcc atatccatca cgaacttctc
      661 gtaactggct actaggccgc cttcaagcca gccggcagtg tgcagaacaa agttagtgcc
      721 agcaaggaaa gttgcatgca tagtcatcaa agtttggtaa ccagcctgag catcaacagt
      781 ttgagaggca tttaagccac cgccaccacg gaacggtaag ccaaagtgac gggcaatctg
      841 tccggtacaa agtaagccga ttgctgattc aggtgtgcca aacataggcg aacctgactg
      901 catgtcaatg ttagaaagga aggaaccaaa aattactggt gaacctgggc gaatgagctg
      961 agatagcgca actccagata gtgcttcagc aatttgttgg accagcgaag ccgggattgt
     1021 tactggtgac attgcaccca ttaacaagaa tggtgtcaaa ataacggctt ggtttgcgcg
     1081 cgtgtattca aattgagatt ccaacatgcg atcatcccag cgaagtgggc tattgcagtt
     1141 aattagtgaa atcgttgtcg gggttttttc gatcgcttct cgaccaccaa aaagtatttc
     1201 gctcattttg attacgtcgc gagcattttg cgccgacaca acattgccca tgtaaacctt
     1261 gtcagtcaga gtttgtaggc gataggtcat atctaagtga cgggtatcta gatgagtatc
     1321 ttctggttca cagataacgc tgcttgctac atcaagatcg gaaaatgact gagttaattt
     1381 caaaaggttt tcgtagtcac gcagtttggc gtcgcggcgc acaccgtctt ctaatacgaa
     1441 aggcgagcca tagttgccag cgaaaaccat actgtcgcca ccaatgtgca cattgttctt
     1501 tggatttcgg gcctgtacat caaattcttt gggtgcctta gcaacttgag ccaaaataaa
     1561 ttctggatcg aacttaacgt tgttaccttc aactttttgt ccggcattgc ggaatagatc
     1621 aagagcttca tcgctaagaa actcgatccc gatttctgag actattcgtt tccagccttt
     1681 gtcgagggct gccattgcat cctgagaaag gatttcgaaa cgcggacttt tattctggaa
     1741 catttgattt ggctcctggt gcgcttataa atattcgcca aaaccccttg acgaacaata
     1801 gtcagagtac actacgatat ggaacggtag ttccaaattg taaaaatttg gaattacctt
     1861 gtccttactt atacgcagtg aacttagacg cagtgaactt ttacgcagta aatcactcgt
     1921 attaataagg tacaaaaccg gccaaagtga cagaggaaca atggaagata aaaccgtaac
     1981 tggtacccaa tccattgatc gtgcctgtga ccttttaatc cgagtaatcg attctgagaa
     2041 accacaaact ttaagtgagt tagtttcagc aactggatta gctaaaggca ccacatctcg
     2101 catccttaat gcactcgagc gagccggcct attgggtcgc agtaacttag gtggcttcac
     2161 tccaggacct gtactcaatc aattcgcagt tgccggcggc gcctacacag cacttgttaa
     2221 atcagtaact ccagctatgg aaagtattgc cgcgcaaact aaagaaacag
//
LOCUS       DBNHVN010000219         3186 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_263899, whole genome shotgun sequence.
ACCESSION   DBNHVN010000219 DBNHVN010000000
VERSION     DBNHVN010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_263899"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /gene="thrB"
                     /locus_tag="AC16Y1_04140"
     CDS             <1..842
                     /gene="thrB"
                     /locus_tag="AC16Y1_04140"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIR5831684.1"
                     /translation="PGYDSLALALDIRDIVTAKPNDSGQIEVEISGQGEDTLPRDESH
                     LIAHTILTTAKKFNLNISGFTLECENNIPQGRGLGSSAAAVVAGLVLARELAKSDLPD
                     ELLLQEATLIEGHPDNVSACLMGGLTISWQTESSEVNTRSINVNPAIVPIVGIPNTEL
                     STKLARNLIPTQIPHVDAVFNSARTALLVAALVADPTSLFDATSDRLHQEYRRSAYPD
                     SMTLVDALRTKEIAACISGAGPTVLALTTKENVPMVIGQITSAGFAARQVSVADTGVN
                     VHN"
     gene            997..2610
                     /gene="rho"
                     /locus_tag="AC16Y1_04145"
     CDS             997..2610
                     /gene="rho"
                     /locus_tag="AC16Y1_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006130144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR5831685.1"
                     /translation="MTDTVALDKGLNNKLLPELRAMADDLGIDGATGMRKSELIEAIS
                     AKQSKGRRSASRAAGRPERNAAEADNDNDSDSSEDSSDRSDRGDRSDRGDRSDRGDRS
                     DRGDRSDRGDRDDRGGRNRRGRDRDRGNRRDRGNRGDNAYELEITEDDVLLPVGGIVD
                     ILDNYAFVRTSGYLPGPNDVYVSMGLVKRFGLRKGDAVTGQVKQPREGDRPQKFNPLV
                     RVESVNGLEPDEVKGRVDFSKLTPLYPQDRLRLETQSNNLVTRVIDLVAPIGKGQRGL
                     IVSPPKAGKTMILQNIANAIVENNPEVHLMVVLVDERPEEVTDMQRSVKGEVIASTFD
                     RPAEDHTTIAELAIERAKRLVEMGHDVVVLLDSMTRLGRAYNLAAPASGRILSGGVDS
                     AALYPPKKFFGAARNIEEGGSLTILATALVETGSRMDEVIFEEFKGTGNMELKLDRRL
                     ADRRVFPAVDIEASGTRREEILLAPEELKIIWKLRRVLHALDQQSGIELLLSKLKETK
                     SNFEFLSQVQKTTPEAPNGMGSDELENID"
     gene            2680..>3186
                     /locus_tag="AC16Y1_04150"
     CDS             2680..>3186
                     /locus_tag="AC16Y1_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HIR5831686.1"
                     /translation="MPSQLRYLFIAISLSVVSMSIAFDFAKPPPPETGCVEIIEIGKS
                     VSGKTIELCHVGGSHIGSKNSMLVIGSVHGNEPAGISVVEELIKLGAGKNTDLWVIKN
                     ANPDGTQLSTRQNSNGVDLNRNFPANWYPSEVGAKTYSGTNPASEPETQSLMRAIDLV
                     KPKILITIH"
BASE COUNT          902 a          697 c          745 g          842 t
ORIGIN      
        1 gtcctggcta tgacagtttg gctcttgcct tagatattcg cgatatagtt actgccaagc
       61 caaatgattc aggccagatt gaagttgaga tttctggcca aggcgaagac acactaccca
      121 gggatgaaag ccatttaatc gcccacacaa ttttgactac tgcaaaaaaa tttaatttaa
      181 atatttctgg tttcacactt gaatgtgaaa ataacattcc gcaaggtcga gggcttggct
      241 cttcagcggc agcagtagtt gctggcttag tgctggcaag agaacttgct aaatcagatt
      301 tacctgatga attattactg caagaagcaa ctttgatcga gggacatcca gacaatgtat
      361 ctgcttgttt aatgggtggc ttaactattt cttggcagac ggaatcaagt gaagtaaaca
      421 cccggtcgat aaatgtgaat ccggcgatag ttccgattgt tgggattcca aatacagaac
      481 tttcgacaaa gttagcgcgt aatttaattc caacccagat cccacacgtc gatgcagtgt
      541 ttaatagtgc tagaactgcg ttacttgttg ccgcattagt tgctgatcca acctcacttt
      601 ttgatgccac ttcagatcgt cttcatcagg aataccgtag aagcgcctat ccagattcaa
      661 tgacactggt cgacgccttg cgcacaaaag aaattgcagc atgtattagt ggagctgggc
      721 ctactgtgtt ggcgctgact accaaggaaa atgtacccat ggtaattggc cagatcacct
      781 cggctggttt tgcagccaga caagtttcgg ttgccgatac cggggtaaat gtccacaact
      841 agcgccttaa tttcacagtt tcgtgaaata cctgctacgc ttttgtcatc ggatgcgaat
      901 tcttcgccca ccgaaatccc actaaagttc gcggataaat tcgcctgtag tgctggatga
      961 agaaatccaa ctatttaaag taaaaaggac acataagtga ccgacacagt cgccctagat
     1021 aaagggttga ataacaaact gcttcctgaa cttcgtgcaa tggccgatga cctaggcata
     1081 gatggtgcca caggaatgcg aaagtctgaa ctaatcgaag ctatctcggc aaagcaaagt
     1141 aaaggccgcc gttctgcctc cagagcagca ggacgacctg aacgtaatgc agccgaagcc
     1201 gataatgata atgattctga ttcaagcgaa gattcttctg accgttctga tcgtggtgac
     1261 cgttctgacc gtggtgaccg ttctgaccgt ggtgaccgtt ctgatcgtgg cgatcgttct
     1321 gatcgtggcg atcgtgatga ccgcggtggt cgaaaccgca gaggtcgcga ccgtgatcgc
     1381 ggtaaccgcc gtgaccgtgg caaccgtggc gataacgcat atgaattgga aatcactgag
     1441 gacgatgtct tactgccagt aggtggaatc gtcgacatcc ttgataacta cgcattcgtg
     1501 cgcactagcg gttatttgcc aggtccaaat gatgtttatg tatcaatggg cttagttaag
     1561 cgctttggct taagaaaagg tgacgcagtc actggacaag ttaaacaacc tcgtgaaggt
     1621 gaccgcccgc aaaagttcaa tccgttagtt cgagttgaat cagttaatgg ccttgaacca
     1681 gatgaagtta aaggtcgcgt tgatttcagt aaattaactc cgctctatcc acaagatcga
     1741 ctacgcctgg aaactcaatc caacaactta gtcacacgcg ttattgattt agtcgcacca
     1801 atcggtaaag gccagcgcgg gttaattgtt tcgccaccta aagctggcaa gaccatgatt
     1861 ttgcagaaca ttgcaaatgc cattgtcgaa aataatccag aagttcattt aatggttgtt
     1921 ctcgttgatg aacgtcccga agaagtcacc gacatgcagc ggtctgtaaa gggcgaagta
     1981 attgcttcaa cttttgatcg accagcagaa gatcacacga ctatcgcaga gttagcgatt
     2041 gagcgtgcaa aacgtttagt tgagatggga catgacgtcg tagtgttact tgactcaatg
     2101 actcgtcttg gccgtgctta caacttggca gctccagctt ctggccgcat tctttctggt
     2161 ggtgttgatt cagcagcgct ttatccacct aagaaattct tcggtgcagc gcgaaacatt
     2221 gaagaaggtg gctcgttaac aattttggct acggctttgg ttgaaactgg ctcgcgcatg
     2281 gacgaagtta ttttcgaaga attcaagggc accggaaata tggaacttaa actggaccgc
     2341 agacttgcag atcgccgagt tttcccagcc gttgatatcg aagcatcagg tactcgccgt
     2401 gaagaaatct tgttggctcc tgaagaacta aagattatct ggaaactacg tcgagtactt
     2461 cacgcacttg atcagcagtc aggtatcgag ttacttcttt ctaaacttaa agaaacaaag
     2521 agtaactttg aatttttgtc ccaagttcaa aagacgacac ctgaggcacc aaatggtatg
     2581 ggttcagatg aactagagaa tattgactag tcaaaattga ctgaaatggc gcagagacaa
     2641 tctctgcgcc atttttgttt tgctggatat cattgggcta tgcccagtca gcttcgctac
     2701 ttattcattg cgatttcgct gagcgttgtt tccatgtcta tcgcctttga ctttgccaag
     2761 ccaccaccac ccgaaactgg ctgtgttgaa ataatcgaaa ttggaaagtc tgtttcgggc
     2821 aaaacaattg aactttgcca tgtcggcgga tcgcacattg gttcaaaaaa ttccatgctg
     2881 gtaatcggat cagtgcatgg aaacgaacca gcgggaattt cagttgtaga agaactgatc
     2941 aaacttggcg ccggtaaaaa taccgattta tgggttatta aaaatgccaa tcctgatggc
     3001 acgcaattaa gtactagaca aaattcaaat ggcgttgacc taaatcgaaa ttttccagct
     3061 aactggtatc caagtgaagt gggtgcaaaa acatatagtg gtacaaatcc agcaagtgaa
     3121 ccagaaactc aaagtttgat gcgggcaatt gatttagtta aaccgaaaat tctgatcaca
     3181 attcat
//
LOCUS       DBNHVN010000220         3619 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1452391, whole genome shotgun sequence.
ACCESSION   DBNHVN010000220 DBNHVN010000000
VERSION     DBNHVN010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1452391"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="AC16Y1_04155"
     CDS             <1..416
                     /locus_tag="AC16Y1_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5831687.1"
                     /translation="PELQDGFFFRPTVLDNCNTKMNCVQDESFGPIMTIEVFDTEDEA
                     IALGNDTIYGLAGAVWTKDSEKAQRVARQLRHGTIWINDFHPYVAQAEWGGYKQSGIG
                     RELGEAGLAEYLEHKHIWQNRSPARSDWFADPKGS"
     gene            419..1453
                     /locus_tag="AC16Y1_04160"
     CDS             419..1453
                     /locus_tag="AC16Y1_04160"
                     /EC_number="7.6.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015697 - quaternary ammonium group
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary amine ABC transporter ATP-binding
                     protein"
                     /protein_id="HIR5831688.1"
                     /translation="MTNGSSAISVRNLWQVFGPNPEKIIGSPDASLPRAELRAKTGNT
                     AAVRDVSFDVAPGEVFVVMGLSGSGKSTLVRCLTRLIEPTSGQIVMAGEDILKASQSR
                     LRELRRTRFSMVFQNFGLLPHRKVIDNIAYALEINGVNKEQRHARAREVIDLVGLQGY
                     DQAYPEQLSGGMQQRVGLARALAVDPEVMFLDEPFSALDPLIRRDMQTEVMRLHRELG
                     KTMIFITHDLDEALKVGDRIAIMRDGAIVQMGTAEELIGNPVDDYVADFTRDVPKSHV
                     LTVKSIARPLATGELPSDRSVSADTIIREATPIVLGSPGPVTVTQNGNSIGVINSNDI
                     LRLIGGGAHS"
     gene            1450..3423
                     /locus_tag="AC16Y1_04165"
     CDS             1450..3423
                     /locus_tag="AC16Y1_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5831689.1"
                     /translation="MSNEVLVTSGRRGIKRSWIFAGLLVLWFALSRIFSGKFTLEISV
                     PQNTVFTTLVGDIADSIRGNRTESAAFKYFFTPVREFIDGFVTIIRQTISIPMPGSSI
                     PVIGWFGVIALIGFIVYAISNVKTALLAVSLTFACGALGLWSDSMDTLAMTLAAVVLS
                     LAIGIPVGVWAGRSDFALKLVTPILDLAQILPTLVYLAPLALFFLIGPASATIATMVY
                     SIPIAIRLTAFGIRGVAKSPVEASVSMGSTGRQTLTKVQLPMAKKTIVLGINQTVMAA
                     LSFVVIAALIGAPGLGKPVLDALKIRNVGEGFVAGFAVVFLAIMLDRATSAAVVKQNT
                     FVPPTPKQLRNRRIALTAAGVGTIVSVFLSRQFAWAAFFPEQLVISKPVANVSDGFVT
                     WVTTSLSFFTEGLIKVVTVGILNPLEATLSNAPWFLTVAAITAISALFGGFRSALVTV
                     TTLLLIVGSGLWYDTMITFAQVLVATLLTMGVGVLLGIWIGRSDKAEKFLRPILDAGQ
                     VLPAFVYLIPMLGFFGPARFTAIATGIVYSIPVVVKIVGQGIREVPVTIIEAATSAGS
                     TTRQLITKVQLPAAKNSLLLATNQGLIFVLAVVVIGGFVGAGGLGYLVLLGSSKPELQ
                     GKGLVAGFAILLLGIMIDRIAQYTVRRNSAPRN"
BASE COUNT          904 a          805 c          873 g         1037 t
ORIGIN      
        1 aaccagaact acaggacggc tttttcttcc gccctaccgt gctcgataat tgcaacacga
       61 aaatgaattg cgtccaggac gagtcattcg gcccaatcat gacaattgag gttttcgata
      121 ccgaagatga ggcgattgct ctaggcaatg acactattta tggtttagct ggagcggttt
      181 ggaccaaaga ttctgaaaag gcccagcggg tggcccgcca acttaggcac ggcactattt
      241 ggattaacga ttttcaccca tatgtcgctc aagcagagtg gggtggatac aaacaatctg
      301 gcataggccg agaattaggt gaggctggat tggcagaata tctagaacac aaacatatct
      361 ggcagaaccg ttcccctgcc aggtctgact ggtttgcaga cccgaagggc agctaacaat
      421 gactaatggt tcaagcgcaa tttccgtaag aaatttatgg caagtttttg gtcctaatcc
      481 cgaaaaaata attggcagtc ccgatgcatc gttacctcga gccgagttac gtgcaaagac
      541 aggtaacaca gctgcagtcc gtgatgtttc attcgatgtt gcaccaggtg aagtgttcgt
      601 cgtgatgggt ttgtcgggtt caggtaaatc aactttggtt cgatgtttaa ctcgacttat
      661 tgaaccaacc tccggtcaga tcgttatggc tggtgaagat attctcaagg catcacaatc
      721 tcgattgcgc gaacttcgcc gcactagatt ttcaatggtt tttcaaaact ttggtttact
      781 tccgcatcgt aaagtcattg acaatattgc ctacgcgtta gaaatcaatg gagttaacaa
      841 agagcagcgc catgctcgcg cacgcgaagt tatcgattta gttggtttac aaggttatga
      901 ccaggcttat ccagaacaac tttctggtgg catgcagcag cgagttggtt tagcgcgcgc
      961 acttgcagtt gatcctgaag ttatgttttt ggacgagcca ttctcagctc ttgatccact
     1021 aatccgtcgt gacatgcaaa ctgaagttat gcgcttacac cgcgaacttg gtaaaaccat
     1081 gatctttatc acgcatgact tagatgaagc tttgaaagtt ggtgatcgaa ttgcaattat
     1141 gcgtgatggt gccattgtgc agatgggtac ggctgaagag ctgattggaa atccggtaga
     1201 cgactatgtc gctgacttta ctcgcgatgt cccaaagtca catgtattaa cagttaaatc
     1261 aatagctcgg ccgctagcaa caggtgaact tccatcagat cgcagcgtga gtgctgacac
     1321 cattattcgt gaagcaacac caatcgtgct gggatcacca ggcccagtta cggttactca
     1381 aaatggcaat tcgatcggag taatcaattc caatgacata cttcgcctaa ttggtggtgg
     1441 agcacactca tgagtaacga agtactagtt acttcaggac gtcgaggaat taagcgtagt
     1501 tggatttttg ctggtctact tgtgttgtgg tttgctctgt ctcgtatttt tagtggcaaa
     1561 ttcactcttg aaatttcggt tccacagaac actgtgttta ccacgcttgt cggggatatc
     1621 gccgactcga ttcggggaaa tcgcacagag agcgcagctt ttaagtactt cttcacgccg
     1681 gttcgcgagt ttattgatgg cttcgtgacc attattcgcc aaactatttc gattcctatg
     1741 ccaggatctt caattccagt tatcggatgg tttggcgtaa ttgcattaat cggctttatt
     1801 gtctacgcaa tttcaaatgt taagactgcc ttattggcag tatcgcttac ctttgcttgc
     1861 ggagcacttg gactttggag tgactcgatg gacacgcttg cgatgacact tgctgcggtg
     1921 gtgctctctc ttgcaatcgg aattccagtg ggtgtctggg caggtcgatc tgacttcgca
     1981 ctgaagttag tcacacccat tcttgatctc gctcaaattt tgccaacttt ggtttacctt
     2041 gctccactag ctttgttttt cctaatcggt ccagcatcgg ccactatcgc aacaatggtt
     2101 tacagtattc caattgcaat aaggctcaca gcgttcggta ttcgtggcgt agcaaaaagt
     2161 ccagttgaag caagtgtctc aatgggttca actggtcgac aaacacttac caaagtgcaa
     2221 ttgccaatgg caaagaaaac tatcgtgcta ggtatcaatc aaactgtgat ggctgcacta
     2281 tctttcgtgg taattgcagc gttaatcggt gcacctggcc ttggtaaacc agtacttgat
     2341 gcactaaaaa ttcgaaatgt tggcgaaggt ttcgtagctg gtttcgctgt tgttttcttg
     2401 gcaattatgt tggaccgagc cacatctgct gctgtggtta aacaaaatac ttttgtgcca
     2461 ccaactccaa agcaacttcg caatcgccga atcgcactaa ctgctgctgg tgttggaact
     2521 atcgtgtcgg tatttctatc acgtcaattt gcttgggccg catttttccc agaacaatta
     2581 gtgatctcaa aaccagtagc gaatgtttca gacggatttg tgacttgggt gacgacgtca
     2641 ctttcattct tcactgaagg actaattaaa gttgttacag ttggaatttt aaatccgctt
     2701 gaagcaacac tttctaatgc accctggttt ttaaccgtgg cagccatcac tgctatctca
     2761 gctcttttcg gtggctttag atcagccctt gttacggtca ctaccttgct tttgatcgtg
     2821 ggtagcggtc tttggtacga caccatgatt actttcgctc aagtgcttgt agccactttg
     2881 ctcacaatgg gtgttggagt tctcttaggt atatggattg gtcgaagtga caaagccgag
     2941 aaattcttac gcccgatttt ggatgctggc caagtacttc cagcatttgt gtacctaatt
     3001 ccgatgcttg gtttctttgg gccagctcgg tttaccgcaa tcgcaacggg cattgtttac
     3061 tcaatcccgg tggtggtaaa gattgtcggc caaggaatta gggaagtccc cgtaacaatc
     3121 atcgaagctg caacttctgc aggttccaca acaaggcaat tgataacgaa agtacaactt
     3181 ccagcagcaa agaactccct tttgctcgca actaatcaag gattgatatt cgtactcgcc
     3241 gttgttgtaa ttggtgggtt cgtgggagct ggaggtttag gctacttagt acttcttgga
     3301 agttcgaaac cagaacttca aggcaagggc ttggtggctg gctttgcgat tttgttgctt
     3361 ggcatcatga tcgacagaat cgcccagtac acggtgcgaa gaaattcagc accacgaaac
     3421 taaaagtttc gaactgacaa acgttggttc gaagggttac cgcaaggtgg cccatttcct
     3481 agcgaggaga gaaagtatgg tccggagaaa aggaaagggc tttagacttg cagcacttac
     3541 tgcagttgct ggcctagcct taacggcctg tggtggagcc ggcgttgatg acgcagctac
     3601 atctacagac acaggtgct
//
LOCUS       DBNHVN010000221         1226 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1081445, whole genome shotgun sequence.
ACCESSION   DBNHVN010000221 DBNHVN010000000
VERSION     DBNHVN010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1226)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1081445"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="AC16Y1_04170"
     CDS             <1..1028
                     /locus_tag="AC16Y1_04170"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR5831690.1"
                     /translation="LLIYGTLPTPAQLTEFENNVSKHTMVHEELRRFFDGFPRDAHPM
                     PVLSSAVSALSTFYQDSLDPFDPQQVEISTIRLMAKLPTLAAYAHKKTIGQPYMYPNN
                     DLGYVANFLAMTFGVPAEKYVVDESIVKALDMLFILHADHEQNCSTSTVRLVGSSQAN
                     LFASISAGINALFGPLHGGANQSVLEMLTEIHESDDSVEDYIKRVKNKEAGIKLMGFG
                     HRVYKNYDPRASIIKKQADAILSRMGIEDPLFDIARKLEEAALADDFFIERKLYPNVD
                     FYTGLIYRAIGFPTRMFTVLFALGRLPGWIAQWHEMINDPDTKIGRPRQVYVGEAERP
                     YLPINKR"
     gene            complement(1032..>1226)
                     /locus_tag="AC16Y1_04175"
     CDS             complement(1032..>1226)
                     /locus_tag="AC16Y1_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007464850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HIR5831691.1"
                     /translation="CVVEAGCYLTGGSKITLPDGQVVKAKELSGASNLMFWRNSVNGA
                     LEAKPKTGSWSGLNSILHSN"
BASE COUNT          348 a          274 c          246 g          358 t
ORIGIN      
        1 acttacttat ttatggcacc ttgccaacac cagctcagtt aactgaattt gaaaataatg
       61 tcagtaagca cacgatggtg cacgaagaac tacgtcgctt ctttgatggc ttcccgcgcg
      121 atgcccaccc aatgccagtt ttaagttcag cagtcagtgc attatctact ttttaccaag
      181 actcactaga tccgtttgat ccacagcaag tagagatctc gactattcgc ttgatggcta
      241 aacttccaac tttggcagcc tatgcgcata agaaaactat tggccagcca tacatgtatc
      301 caaataatga tttaggttac gtggctaact tcttggcgat gacttttggc gtcccagctg
      361 aaaaatacgt tgtagacgaa tcaattgtta aagcattaga catgctgttt attttgcatg
      421 ccgatcatga gcagaactgt tcaacctcaa ctgttcgtct cgttggttct tcgcaggcga
      481 atttatttgc gtcaatctca gccgggatta atgcactatt tggtccactg catggtggcg
      541 caaaccaatc tgtattagaa atgcttactg agattcatga gtctgacgat tcagtcgagg
      601 attacataaa gcgggtaaag aataaagaag ccggcattaa attaatgggc tttggtcacc
      661 gcgtctataa gaattacgat ccacgtgctt cgattatcaa aaaacaagca gatgcgattt
      721 tgagtcgaat gggtatcgaa gatccgctat tcgatattgc ccgcaaacta gaagaagctg
      781 cgctagcaga tgacttcttt attgagcgca aactttatcc aaacgttgat ttctacaccg
      841 gcctgattta tcgcgcgatt ggttttccaa ctcgaatgtt cactgtgtta tttgcacttg
      901 gtcgcttgcc aggttggatc gcgcaatggc acgaaatgat taatgatcct gataccaaaa
      961 ttggtcgacc acgtcaggtt tatgttggcg aagcagaacg cccttactta ccaattaaca
     1021 aacgttaaaa attagtttga atgcaaaata ctatttagac cagaccagct gccagtcttt
     1081 ggttttgctt ccagtgcgcc attaactgaa ttacgccaaa acattaagtt acttgctcca
     1141 gataattctt tggctttcac aacttgacca tctggcaacg taatttttga accaccagtt
     1201 aagtagcaac ctgcttcaac aacaca
//
LOCUS       DBNHVN010000222         2373 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1453219, whole genome shotgun sequence.
ACCESSION   DBNHVN010000222 DBNHVN010000000
VERSION     DBNHVN010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1453219"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1231)
                     /gene="dxs"
                     /locus_tag="AC16Y1_04180"
     CDS             complement(<1..1231)
                     /gene="dxs"
                     /locus_tag="AC16Y1_04180"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIR5831692.1"
                     /translation="MLDRINSPADLKGLTLDELNKLAVEIREFLVSKVAKTGGHLGPN
                     LGVVELTLAIHRVFESPKDPILWDTGHQSYVHKIVTGRANQFDSLRQQDGLSGYPSRE
                     ESIHDVIENSHASTALSYADGIAKAFEIHGEKDRHVVAVVGDGALTGGMTWEAINNIA
                     DGSQRSIVIVVNDNERSYSPTIGGMAHHLATLRTTKGYEKFLEWGKGVLERAPVGGHI
                     VFEALHGMKKGIKDFVAPQGMFEDLGIKYIGPVDGHNIEELEFALERARDFGAPVIVH
                     AITEKGRGYRPAEQDDAEKFHAVGVVDPEDGKPLKESVVSWTNIFSDELVSLGDKRQD
                     IVAITAAMLGPTGLDKFKANFPNRTFDVGIAEQHAVTSAAGMAHAGLHPIVAVYSTFL
                     NRAFDQVVMDCALHNAGV"
     gene            complement(1321..>2373)
                     /gene="acnA"
                     /locus_tag="AC16Y1_04185"
     CDS             complement(1321..>2373)
                     /gene="acnA"
                     /locus_tag="AC16Y1_04185"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489101.1"
                     /note="catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HIR5831693.1"
                     /translation="LASPHLVVAYAIAGSMDVDLAKDPLGNDQDGNPVYLKDIWPSVD
                     EVQSVIDQSIDPQMFTARYRDVFAGDERWKNLNTPTGDTFAWDPKSTYVRRPPYFENM
                     PAQPTPVQDINGARVLAKLGDSVTTDHISPAGSIKVDSPAGKYLSENGVDKKDFNSYG
                     SRRGNHEVMIRGTFANIRLKNQLLDGVEGGFTRDFTKPDAPQTTIYDASVAASSAGIP
                     LIILAGKEYGSGSSRDWAAKGTALLGVKAVIAESYERIHRSNLIGMGVLPLEYPEGQN
                     ALSLGLTGEETFTFTGIIKLNEGQTPKTVKVLAKGVNGTETEFDAVVRIDTPGEADYF
                     RHGGILQYVLRSLRAA"
BASE COUNT          637 a          591 c          475 g          670 t
ORIGIN      
        1 tgacaccagc attatgtagt gcgcaatcca taacaacctg atcaaatgca cgatttagga
       61 aagtactgta aacagctact atcggatgca gccctgcatg agccataccc gctgcacttg
      121 tcaccgcatg ctgctcggcg ataccaacat caaaagttcg attaggaaaa ttagccttga
      181 acttatcaag accggtcgga ccaagcatcg cagccgtgat tgccacaata tcttggcgtt
      241 tgtcaccaag tgaaactaat tcatcactaa aaatatttgt ccaagaaacg actgattctt
      301 ttagtggctt accgtcttct ggatcaacga ctcccactgc gtgaaatttc tcagcatcat
      361 cttgttcagc tggacgatat ccgcgacctt tttcggtgat tgcatgaaca ataactggtg
      421 cgccaaaatc tcgtgcccgt tcaagtgcaa attccaactc ttcgatgtta tgaccatcta
      481 cggggccaat gtatttgata ccaagatctt caaacattcc ctgcggcgca acaaaatctt
      541 taattccctt tttcatgcca tgcaaggctt caaaaacaat gtgtccacca actggtgcac
      601 gttctagaac acctttaccc cattcaagaa atttctcata tcctttagtg gttcgaagtg
      661 tcgctaaatg atgtgccatg ccgcctatcg ttggcgaata cgatcgttca ttgtcattga
      721 ccacaattac gattgagcgt tgtgagccat ccgcaatgtt attaatggct tcccaagtca
      781 tcccaccggt cagtgcaccg tcgcctacta cggcaacaac atgtcgatcc ttttcaccgt
      841 gtatttcaaa tgccttagct attccatctg cgtaagataa cgctgttgaa gcatggctat
      901 tttcaataac gtcatgaata ctttcttcgc ggcttggata accagacaag ccatcttgct
      961 ggcgaagtga atcgaattga ttagctcggc ccgtcacaat tttgtgcacg tatgactgat
     1021 gaccggtatc ccaaagaatt ggatcttttg gtgactcgaa aactcggtgg atggctaacg
     1081 ttaattcgac tacacctaga tttggaccaa gatgcccacc agtttttgca accttagata
     1141 ctaagaattc gcgaatttcg accgcaagtt tatttagctc gtctagagtc aagcccttta
     1201 aatcggcagg tgaatttatc cgatctaaca cgtattcacc cttcggttaa ggtctacaga
     1261 ccggagcctt tagaccctac caagtttact tgcgacttga aatccgtaca ttaagaggat
     1321 ttacgctgcg cgcaaactgc gtaaaacgta ttgcaaaatc ccaccgtgtc tgaagtaatc
     1381 ggcttctccc ggtgtatcaa ttcgcactac ggcatcaaat tcagtttccg tgccgttgac
     1441 gcccttagcc aaaactttga cagtttttgg tgtttgacct tcgtttagtt taattatccc
     1501 agtaaacgta aaagtttctt ctccagttaa accaagtgac aaggcattct gaccctcggg
     1561 atattcaagt ggaagtacgc ccataccaat caaatttgac cgatgaatcc gctcgtaaga
     1621 ttccgcaatc acggccttaa ctccgagtag ggctgtgccc tttgctgccc aatctcgaga
     1681 tgagccggaa ccgtattctt tgccagcaag aattattagt ggaattccag cagatgaagc
     1741 agcaaccgaa gcatcgtaaa tagttgtttg tggagcatct ggcttagtga aatctcgagt
     1801 gaatccacct tcaactccgt cgagcaattg atttttcaac cggatgtttg caaaagttcc
     1861 acgaatcatt acttcatgat ttccacgacg cgagccgtat gaattgaaat ctttcttgtc
     1921 aacgccgttt tcagacaagt acttccccgc tggactatcg acttttatcg acccagcagg
     1981 cgaaatgtga tcggtggtaa cactgtcccc taatttagct aaaacccgtg cgccgttaat
     2041 gtcttgtacc ggcgttggct gtgccggcat gttttcaaaa tatggaggac ggcgaacata
     2101 agtactcttt ggatcccaag caaacgtatc cccggttggt gtatttaagt ttttccatct
     2161 ttcatcgcca gcaaaaacat ctcggtatct agcggtaaac atttggggat cgatcgactg
     2221 atcaatcacg ctttgtactt catcaacact tggccaaata tcttttagat aaaccggatt
     2281 accatcttgg tcgttcccaa gtggatcttt tgctaaatca acatccatgg agccagctat
     2341 cgcatacgcc acaactaaat gtggtgaagc aag
//
LOCUS       DBNHVN010000223        17903 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1898961, whole genome shotgun sequence.
ACCESSION   DBNHVN010000223 DBNHVN010000000
VERSION     DBNHVN010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 17903)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17903)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17903
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1898961"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="AC16Y1_04190"
     CDS             <1..612
                     /locus_tag="AC16Y1_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003967081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D-related
                     protein"
                     /protein_id="HIR5831694.1"
                     /translation="ILDLAGCMALGMTGPVLRATGLPQDLRRTDPYCGYETYEFDVIT
                     KDTCDVYGRFLIRLQEMRESLKIIEQCLDRLKPGPVMVEDKRIAWPAQLSIGSDGQGN
                     SLQHIRHIMGESMEALIHHFKLVTEGFQVPAGQAYSAVESPRGELGVHVVSDGGTRPY
                     RVHFRDPSFTNLQAVAAMCEGGQVADVIASVASIDPVMGGVDR"
     gene            612..1256
                     /gene="nuoE"
                     /locus_tag="AC16Y1_04195"
     CDS             612..1256
                     /gene="nuoE"
                     /locus_tag="AC16Y1_04195"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HIR5831695.1"
                     /translation="MALTEETRVKAREIIARYPVPRSALLPMLHLVQAEEGYVTADGI
                     ALCAEELGLTTAEVAAVATFYTMYHRKPVGEYHIGVCINPGCGILGGDEIWSTLCSRL
                     GIGNDEVTEDGKISLERIECQAACTHAPVMTANWEFLDNMTPDKAVNVVDKLRRGEEV
                     LSTRGPRIRTFQENEATLAGFEDDLVDAGGNADELMLAGLKRAKELGMSAPEAK"
     gene            1256..2572
                     /gene="nuoF"
                     /locus_tag="AC16Y1_04200"
     CDS             1256..2572
                     /gene="nuoF"
                     /locus_tag="AC16Y1_04200"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR5831696.1"
                     /translation="MAIAQGSLTPVLSAHWDEPDSYTIAGYMRHGGYTAFKKAFAKGP
                     DDVINTVKDSGLRGRGGAGFPTGLKWSFVPQGDGKPHYLVVNADESEPGACKDIPLML
                     ANPHSLIEGIIIASFAMRANHAFIYLRGEVPHVFRRVNAAIKEAYQAGYLGKNIMGSG
                     FDLEVVLHAGAGAYICGEETALLDSLEGRRGQPRLKPPFPAVAGLYASPSCVNNVETI
                     ANIPYIVNNGVDWFRSFGTEKSPGFKLFAVSGHVARPGIYEAPLGITMRELLEHAGGV
                     RGGHKVKFWLPGGSSVPMLTPDHLDTPLTYEAIAEAGSMLGTGTPMIFDETTSVVRAV
                     TGWLAFYKHESCGKCTPCREGTYWISDVLHRFEEGKGTESDIDKLVEICQQIAGRSFC
                     ALGDAAATPFPAALKYFRDEFLSATKTSANESFDPEKSTIFAGAAK"
     gene            2569..4785
                     /locus_tag="AC16Y1_04205"
     CDS             2569..4785
                     /locus_tag="AC16Y1_04205"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017618587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HIR5831697.1"
                     /translation="MTVTTQTVNVTVDGVTVEVPAGTLIIRVAEMLGTAIPRFCDHPL
                     LDPVAACRACLVEIEGQGKPQPACAIPVAEGMVVKTQHTSEMANKAQAGVMEFLLINH
                     PLDCPICDKGGECPLQNQAMTAGRPETRFDGAKRTFPKPINVSAQVLLDRERCVSCAR
                     CTRFADEIAGDPFLALLERGAKQQVGISADQPFDSYFSGNTIQICPVGALTSAKYRFR
                     SRPFDLISAPTACEHCASGCALRTDVRRDQIMRRLAWDAPEVNEEWNCDKGRFAFTYT
                     NEGRLTTPLVRDEEGNLVPASWPEAIRVAAKGLASSGAKTAVLTGGRLTVEDANAYAS
                     FARNVLSTSDVDFRARATSDEETAFLANFVAGKGLGVTYGDLESAKTVLLVGLEPEDE
                     SPIVFLRLRKAALKRGTKVIAIAPYMTAGFAKMKTKLIAASPRDEVSAVSNVASELGE
                     NSFVLVGERAATTPGLLTAVAELVNRTGAKLAWIPRRAGDRGAVDAGLLPVGGRNTTA
                     IINETGKSIKALVIAGVSAADVDGDLISATDRADFVVSLETRHTDVTANADVVLPVSV
                     VSEKSGTYRNWEGRDRSFKAVLKAPGLLSDAQVLAELAIEMGKPLGENNYWANTQPPQ
                     LVAGDSQSSSIASGQAILSTWKHLLDNGSLQEGEPYLAATARVGVIRVSPATAQNLNL
                     VDGAMAKVSLGSKQAVAPVIVTVGMADGVVWLPTNSSDCAINLPSGTVVNVSAGGA"
     gene            4782..6068
                     /gene="nuoH"
                     /locus_tag="AC16Y1_04210"
     CDS             4782..6068
                     /gene="nuoH"
                     /locus_tag="AC16Y1_04210"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HIR5831698.1"
                     /translation="MMNSIFGNDSGLIIAVKVFGIFAIMVVMTLLTIWAERRVVGRMQ
                     MRIGPNRVGPFGLLQSLMDGIKLALKEEIIPKGVHMAVYVLAPVISATAAFLTFAVIP
                     FGPEVSIFGQLTPLQLTDFPVSVLYVLAIASIGIYGIVLAGWSSGSTYPLLGGLRSTA
                     QMISYEIAMGLSFVAVFLYASSMSTSEIVAAQEPLWYAVLLLPSFAIYVVSMVGETNR
                     APFDLPEAEGELVGGFHTEYSSLKFALFFLAEYVNMVTVSALATTLFLGGWRAPWPIS
                     LWDQANTGWWPMLWFFGKLFAFLFIFIWLRGTLPRMRYDQFMRFGWKVLIPFSIAWIL
                     IVATARYLRSAGAVDTQSLLFAAGVVLVVGLSISYALELRAKKIAAQEELRTTELANK
                     PFDPFEGGFPVPPLPGQEAIPSRHKALSVTAKGEGN"
     gene            6068..6601
                     /gene="nuoI"
                     /locus_tag="AC16Y1_04215"
     CDS             6068..6601
                     /gene="nuoI"
                     /locus_tag="AC16Y1_04215"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011740430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HIR5831699.1"
                     /translation="MAELPQLIRGFGVTFRSMFKKVTTEQYPEEKDKFPPKPRFHGRH
                     QLNRHPDGLEKCIGCELCAWACPADAIYVEGAENSEEERFSPGERYGRVYQINYLRCI
                     LCGLCIEACPTRALTMTNEYELADNSRAKLIYEKQDLLAPIMPGMQAPPHAMAPGTTE
                     TDYYLGNIKGDSARVDS"
     gene            6601..7317
                     /locus_tag="AC16Y1_04220"
     CDS             6601..7317
                     /locus_tag="AC16Y1_04220"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HIR5831700.1"
                     /translation="MNGESVVFWVTAIFAVAGAIGMVVSRKAVHSALWVAFTMINIAI
                     LYFALEAAFLGVTQIVVYTGAVMMLFLFVLMMVGVDSSDSLLETIKGQRLVAVLLVLA
                     FAAMVVGGIAATGVITARGLAGANNFNGGNVQGLAELIFGRYLLAFEVTSALLITAAV
                     GAMLLAHRERITPRKTQAQLQRERVLGGHPTPLPAPGVLATNNAVNVPALLPDGTPAQ
                     ISLPTQFTQPDESNRPEVNK"
     gene            7314..7613
                     /gene="nuoK"
                     /locus_tag="AC16Y1_04225"
     CDS             7314..7613
                     /gene="nuoK"
                     /locus_tag="AC16Y1_04225"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005061219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIR5831701.1"
                     /translation="MNANNYLVLAAILFSIGAFGVLVRRNAIIVFMSVEMMLNAANLA
                     FVAAARITGTLDGQVTAFFVMVVAAAEVVVGLAIIMTIFRTRKSASIDEASLLKY"
     gene            7624..9483
                     /gene="nuoL"
                     /locus_tag="AC16Y1_04230"
     CDS             7624..9483
                     /gene="nuoL"
                     /locus_tag="AC16Y1_04230"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020135932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR5831702.1"
                     /translation="MIAALIGLPLLGAVVLLIGGRKTNSWGHYFAVLMSGASFVLGTS
                     LFVQMLGRGEEERAIKQHLFDWIAVGLFQVPFGLQLDQLSIAFVLLITGVGTLIHIYS
                     IGYMEHDENRRKFFAYLNLFVAAMLLLVLADNYLLLYVGWEGVGLASYLLISFWQHKS
                     TAAAAGKKAFIVNRVGDVGLSLAIMLMFVTLGTVTFDGVAENIANANDTTVTWIGLAL
                     LLAACGKSAQFPLQSWLLDAMEGPTPVSALIHAATMVTAGVYLIARAHSIFDLSQTAR
                     TAVVIIGAITLLLGAYIGTAKDDIKKGLAGSTMSQIGYMILAAGLGPAGYVFAIFHLL
                     THGMFKAGLFLGAGSVMHGMHDDVNMRHYGALRSAMKITYVTFGLGFLAIIGVPPFAG
                     FWSKDEIIYVAFGQNLIIGVATLIGAGITAFYMTRMMTMTFFGEARWQPEVHPHESGK
                     VMTIPMIILAVGSVFGGLLLLFVGDIEHWLEPVVGYAKSEGGISHNTLIPITMSVVLV
                     GAGYAWIKYGRRPVPVAAPTDVSFLTRAARADAYGDAFNESVFMRPGQHLTRALTYFD
                     AKGVDGVVNGLAAAIGGLSARARMTQSGFTRSYALYMLAGAALIAFVLLLVRL"
     gene            9486..11033
                     /locus_tag="AC16Y1_04235"
     CDS             9486..11033
                     /locus_tag="AC16Y1_04235"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HIR5831703.1"
                     /translation="MLLTTLMALPLVGALVVFALPVGRELLAKQIAFAVSLVTALGAI
                     YLGLNLDNSKSGLQFLESYEWIPAFGINWTVGVDGISAVLIVMATILVPLVIIAGWND
                     VERLGQGTVKGYFSLFLLLETFMIGVFAATDLFLFYVFFEAMLIPVYFLIGRFGGPQR
                     AYAAMKFLLYSLVGGLFMLASLIGLYVASGRELGEGTFDFTALANLNLDPATQKILFL
                     GFFFAFAVKAPMVPFHTWLPDAAAQATPATSTLLVGVLDKVGTFGMIRFLLPIFPDAS
                     KYYAPVIIGLAVVSIFYGALLAIGQTDLLRLVAFSSVSHFGFIVMGIFVMTSQGLSGA
                     TLYMVNHGFSTGALFIVVGFLISRRGSKFIADFGGVNKVAPLLAGFVLLSGLSSLALP
                     GMSSFVSEFLVLAGTFAYNKTVAVIATFGIVLAAFYILWMYHRTMTGEPSNEVVQTMT
                     EINRRELAAITPLIALIIALGFAPQVALNVINPAVGQVQDFVDVQDKQPEIKSTGLVE
                     LSMKVGA"
     gene            11033..12601
                     /gene="nuoN"
                     /locus_tag="AC16Y1_04240"
     CDS             11033..12601
                     /gene="nuoN"
                     /locus_tag="AC16Y1_04240"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HIR5831704.1"
                     /translation="MQISIEYAALAPILIVLGAGVISVLLEAFTPRPARRPLQLILVF
                     GSLMLAFVYVVVNSSAPLTGPAEAVGGQTKGIRQIIAQGSVVLDGPGLFIQGILLIIS
                     FIAALMIAERSIDSQGDAFAPRASALPGSEDERAFTAQGVLQTEIWPLYLFSVGGMLL
                     FPVSNDLLTMFIALEVFSLPLYLLAGMARRRRLLSQEAALKYFVLGAFASAFFLFGAA
                     LIYAVTGVMQFGQLAEALANSGQDGLVLGGMGLIAVGLLFKIGAVPFHQWTPDVYQGS
                     PTPITAFMSAATKITAFAALLRVFYVVFGGLRWDWQPVMMVIALLSMIFGSLLAVTQT
                     DIKRMLAYSSVAHAGFLLLGLIAASPNGLSSSLFYLFAYGLTSLGAFGVVSLVRDSSG
                     EATHLSQWAGLGKTSPWIASTFALFLLALAGIPLTSGFTGKFAIFTAAVESGQTPLVI
                     VAVVASAIAAFFYARVIVLMFFTNAPEDAPSVVVPSLLTTIAIGVTAAFTLLLGVVPQ
                     PVLDLCANAGVFLR"
     gene            12668..13645
                     /locus_tag="AC16Y1_04245"
     CDS             12668..13645
                     /locus_tag="AC16Y1_04245"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018840704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR5831705.1"
                     /translation="MTVLFPTNLASLEAALVAGLAAVEKELRSEIKSDYELADITARH
                     LVEAGGKRFRPLMVLLAAQFGDPTAKGVVPSAVVVELTHLATLYHDDVMDEATVRRGT
                     ESANSRWGNTVAILSGDFLFARSSKILADLGPEAVRIQAQTFERLVIGQLRETTGPAP
                     GEDPVKHYLDVLAGKTGSLIAAAGRYGALMSGVNPELTERIARFGENIGIAFQLSDDL
                     LDIKSNNSGKTPGKDLREGIKTLPVLFAIADPNTTPQLRQLLSGPIADDKDHALALAQ
                     LREHRAMDQANDVLLEWVAKARELLSDFPEGEPKAALHVLCDSIVDRDI"
     gene            13651..14571
                     /gene="rarD"
                     /locus_tag="AC16Y1_04250"
     CDS             13651..14571
                     /gene="rarD"
                     /locus_tag="AC16Y1_04250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HIR5831706.1"
                     /translation="MTKQQRGDAAQGVLYGFAAYIIWGIVPIYWPLLQPAGAIEILAH
                     RVIWSLVVLLVFLAIKKRIKKALLIFKDPKKLAILTIASVFIAVNWGIFIWASVSGRI
                     LDSSLGYYINPLFSIGLGVLLLKEKLRNLQWLAIAIATIAIIYLSVTLGAPPYVALSL
                     AFTFSIYGYLKKRANVEAIDSLTVETLILIPFAIGYIAYLSSVGQNSFGVNGIDHAIW
                     LASAGVITAVPLALFGAAAIRIPLSTLGFIQYVGPTLQFIIGLYLFNEPMPRDRFIGF
                     ALTWVAIAIISYDALRNRNKVSKQYVPDLD"
     gene            complement(14568..15644)
                     /locus_tag="AC16Y1_04255"
     CDS             complement(14568..15644)
                     /locus_tag="AC16Y1_04255"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006140990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIR5831707.1"
                     /translation="MPSRMWLQVSDTFPSLALVPKTDEKQSAKDFKTDQEVRWCPGCG
                     DYAILAAVQGFMPELGVKRENIVFVSGIGCSSRFPYYMNTYGVHSIHGRAPAIATGLA
                     TSRPDLSVWVVTGDGDALSIGGNHLIHALRRNVNLKILLFNNRIYGLTKGQYSPTSPL
                     GKITKSTPVGSLDYPFNPLSVALGAEATFVARTIDSDRAHLQSVLRAAAAHEGTALIE
                     IYQNCDIFNDGAFEPLKNSETKDDVTIRLEHGEKIIFGVNRDRCVIRNEIGELEVALV
                     VGVDEAKIVVHDAHHEDPSLAFGLSRLSHPETLQDTPIGVFRDVKRASYDRLMQEQLD
                     DVIAKTGPGDLTKLLLGKETWQVN"
     gene            complement(15613..17475)
                     /locus_tag="AC16Y1_04260"
     CDS             complement(15613..17475)
                     /locus_tag="AC16Y1_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020549296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR5831708.1"
                     /translation="MSKDVVELDQVIIRFAGDSGDGMQLTGDQFTSTTAAFGNDLKTL
                     PDFPAEIRAPAGTLPGVSAFQLNFGDHTIMTPGDQAHVLVAMNPAALKANLKELRLGG
                     DLIVNTDEFGDRAISKVGYKSNPLQDGTLDGYNVHKIALTSMTVEALSEFEITKKEAE
                     RAKNMFALGMLLWLYSRPIEPTLEFLRTKFGKKPQILAANEKALQAGWSFGETTESFA
                     NQYVVKPAKMSPGTYRNISGNLALSYGLIAASVRSKLTIFLGSYPITPASDILHELSK
                     HKNFGVRTFQAEDEIAGIGAAIGASYAGMLGVTTTSGPGVALKSEAIGLAVALELPLI
                     IVDVQRGGPSTGLPTKTEQADLLQAMFGRNGEAPVPIVAPQSPSDCFYAAMEACRIAV
                     TYRTPVFLLSDGYIANGAEPWRVPSEDDLIEIDPKFATGPNHKLPDGTDVFWPFKRDP
                     ETLARPWAVPGVAGLEHRIGGIEKADGTGNISYDPTNHDFMVRTRQAKVDGIAKSIDL
                     LTVDDPSNQAKVLIIGWGSTYGPVNAACEEVRKQGLNIAHAHLRHVNPFPTNLGEVLK
                     KYEKVIIPEMNLGQLALLIRAKYLVDAISYNQVRGLPFKTQELVDAIKDVVASV"
     gene            17596..>17903
                     /locus_tag="AC16Y1_04265"
     CDS             17596..>17903
                     /locus_tag="AC16Y1_04265"
                     /inference="COORDINATES: protein motif:HMM:NF013242.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR5831709.1"
                     /translation="MNSLAVHVFQAGLACCGVEAAAVDLVTQAGVKIVPSDQDANILV
                     VAGTITKVLVPKVIAAYEKLAEPKVVIAFGVCTITGGPYWDSYSVISGAHELISVNIN
                     V"
BASE COUNT         4516 a         3838 c         4233 g         5316 t
ORIGIN      
        1 attctcgatt tagctggctg tatggctctt ggcatgactg gtccggtatt gcgggccact
       61 ggattaccgc aagatttacg tcgtactgat ccatattgcg gatacgaaac ttatgaattc
      121 gatgtgatca caaaagatac ctgtgatgtg tacggtcgct ttttgattcg gttacaagaa
      181 atgcgcgaat cattaaagat tattgaacaa tgtctagatc ggctaaagcc tggaccagta
      241 atggttgaag acaagcgcat tgcttggcct gctcagttat ctatcggttc agacggacaa
      301 ggtaactcgt tacaacacat ccgtcacatc atgggcgaat caatggaagc tttaattcac
      361 cacttcaaat tagtaactga aggttttcaa gttccagctg gtcaggcgta cagcgcagtt
      421 gaatctcctc gcggtgaact tggtgtgcat gtagtttccg atggcggcac ccgcccttat
      481 cgagttcact tccgtgatcc gtcatttaca aacttgcagg ctgttgccgc aatgtgtgaa
      541 ggcggacagg ttgcagacgt tattgcatcc gttgcatcaa tcgatccagt tatgggaggt
      601 gtagatcgct aatggcatta actgaagaaa ctagagtcaa agcgcgtgaa attattgccc
      661 gttatccagt accgcgttca gcactacttc caatgttgca ccttgtgcaa gcagaagaag
      721 gttacgtaac tgcagatgga attgcacttt gtgctgaaga acttggttta acaactgccg
      781 aagttgccgc agtagcaact ttctacacga tgtatcatcg caaaccagtt ggcgaatatc
      841 acatcggcgt ttgcatcaac ccaggctgtg gaattttagg tggcgacgaa atttggtcaa
      901 ctttatgcag tcgccttgga attggcaacg atgaagtaac tgaagatgga aaaatttcac
      961 ttgagcggat tgaatgtcaa gcagcatgca cccatgctcc agtgatgacg gctaattggg
     1021 aatttttaga caacatgacc ccagataagg cagttaatgt tgtcgacaaa ttgcgtcgcg
     1081 gtgaagaagt tttgagtact cgcggacctc ggatccgtac tttccaagag aacgaagcaa
     1141 cacttgcagg ttttgaagac gatctagttg atgctggtgg aaatgctgac gaacttatgt
     1201 tggctggatt gaagcgcgct aaggaacttg gcatgagcgc accggaggca aaataatggc
     1261 gattgcacaa ggttcgttaa ctccagtttt atctgcacat tgggatgaac cagattccta
     1321 cacaattgcc ggttacatgc gccatggcgg atacaccgca tttaaaaagg ctttcgctaa
     1381 aggcccagac gacgttatta atactgtcaa agactccgga ttgcgcggac gaggtggcgc
     1441 tggatttccg accggcctta agtggtcttt cgttcctcaa ggcgatggca agccgcacta
     1501 cttagtggtt aatgctgatg agtctgaacc aggtgcatgt aaagacattc cgctcatgtt
     1561 ggcgaatccg cattcgttaa ttgaaggcat cattattgct tcctttgcaa tgcgggcaaa
     1621 ccatgcattc atttatcttc gcggcgaagt tcctcatgta ttccgtcgag taaacgcggc
     1681 aattaaagag gcgtaccaag ctggatattt aggtaaaaac attatgggat caggctttga
     1741 tctcgaagtt gttttacatg cgggcgctgg tgcgtacatt tgtggtgaag aaactgcgtt
     1801 acttgattcg ctagaaggtc gtcgcggtca accgcgtttg aagccaccgt tcccagccgt
     1861 ggctggactt tatgcttcac cgtcttgtgt gaacaatgtc gaaactattg caaacattcc
     1921 atacattgtt aataacggtg tcgattggtt ccggtcattt ggtacagaaa agtcaccagg
     1981 cttcaaatta tttgcagtct caggacacgt tgctcgtcca ggtatttatg aagctcctct
     2041 tggaataacg atgcgcgaac ttcttgaaca tgctggaggt gtgcgcggcg gacacaaagt
     2101 taagttctgg ttgccgggcg gatcttccgt gccaatgctt acacctgatc atttagatac
     2161 accacttacc tacgaagcaa ttgccgaagc cggttcaatg cttggcactg gtacaccaat
     2221 gatttttgat gagacaactt ctgtcgttcg ggcggtaact ggttggttag ctttctacaa
     2281 acatgaatca tgtggcaagt gcacaccatg ccgtgaaggt acttactgga tcagcgatgt
     2341 acttcatcgt tttgaagaag gtaaaggaac tgaaagcgat atcgacaaac ttgttgaaat
     2401 ttgtcaacag attgctggtc gttcattttg cgcattaggt gatgctgcag caacaccatt
     2461 cccagcagca cttaagtact tccgtgatga atttttatct gcaacgaaaa cttctgcaaa
     2521 tgaatcattt gatccggaaa agtcaactat ttttgctgga gcagcaaaat gactgtaacc
     2581 acacaaactg taaatgtcac cgttgacgga gttaccgtcg aagtgcctgc tggaacatta
     2641 attattcgag ttgctgaaat gcttggcact gcaattccac gtttctgcga tcatccattg
     2701 ttagatccag ttgctgcttg ccgcgcgtgc ttagttgaaa ttgaaggtca aggtaaacca
     2761 caaccggcct gtgcaattcc agttgctgaa ggcatggtgg taaagactca gcacaccagt
     2821 gaaatggcca ataaagcaca agctggtgtg atggagtttc tccttattaa ccacccactt
     2881 gattgtccga tctgcgacaa aggtggcgaa tgcccattac aaaatcaggc gatgacagct
     2941 ggccgaccag agactcggtt cgatggcgct aagcgaactt tccctaaacc aataaatgtt
     3001 tccgcacagg tgttactcga tcgcgaacgt tgcgtatctt gcgcgcgctg tactcgattt
     3061 gctgatgaaa ttgctggcga tccgttcttg gcactacttg aacgcggcgc aaagcagcag
     3121 gttggtattt cagcagatca accatttgat tcttatttct ctggaaacac aattcagatt
     3181 tgtccggttg gcgcactaac tagtgcaaaa tatcggttcc gttctcgtcc gtttgatttg
     3241 atttcagcac caactgcgtg tgagcactgc gcgagtggct gtgcgttacg cactgacgtt
     3301 cgacgtgatc aaattatgcg ccgcttagcg tgggacgcac ctgaagtaaa tgaagaatgg
     3361 aactgcgaca aaggtcgctt cgctttcact tatacaaatg aaggccgctt aaccacgcca
     3421 ctagtgcgcg atgaagaagg taatttagtt ccagcctcgt ggccagaagc gattcgagtt
     3481 gccgctaaag gtttagcaag ttctggtgca aaaactgctg tgttaaccgg cggccgacta
     3541 accgttgaag atgcaaatgc ttatgcgtcg tttgcgcgca atgtactttc aacatcagat
     3601 gttgatttcc gggcgcgcgc aactagcgat gaggaaactg cattcctagc taactttgtt
     3661 gccggcaaag gcttaggcgt aacttacggc gacctagaaa gtgccaaaac tgttttactt
     3721 gttggcttag aaccagaaga tgagtcacca atagttttct tacgtcttcg caaagctgca
     3781 ctgaaacgtg gaaccaaggt aattgcaatc gcaccttaca tgactgcagg ttttgccaag
     3841 atgaaaacca aactaattgc cgcaagcccg cgtgacgaag taagcgcagt aagtaatgtg
     3901 gctagcgaac ttggtgaaaa tagttttgta cttgttggtg aacgtgcagc aacgacgcct
     3961 ggcttactca ctgcagttgc cgaattagtt aatcgaactg gggcaaagtt agcttggata
     4021 cctcgccgtg ctggtgatcg tggcgcggtc gatgctggac tgttacctgt tggtggacgt
     4081 aacacgactg cgattattaa tgaaaccggt aaaagtatca aagcgttagt aattgcagga
     4141 gttagtgcag cagacgttga tggcgatttg attagtgcca ctgatcgtgc ggacttcgtg
     4201 gtttcacttg aaactagaca cacagatgtc actgcaaacg cagatgttgt ccttccagtt
     4261 tccgtagttt cagaaaaatc gggaacttac cgtaactggg aaggtcgcga tcgatctttc
     4321 aaagcagttt taaaggcgcc aggcttactt agtgatgcac aagtgcttgc tgaactagca
     4381 atcgaaatgg gtaagcctct tggcgagaat aactactggg ctaacactca gccaccgcaa
     4441 cttgtagctg gcgattcaca aagcagttca atcgcaagtg gacaagcgat attgagtacc
     4501 tggaaacatt tacttgataa cggaagtttg caagaaggtg aaccatatct agctgcaact
     4561 gctcgagttg gagtaattcg agtcagtcca gcaactgcac aaaatttgaa tttagttgat
     4621 ggtgccatgg caaaagtttc acttggtagc aagcaagcag ttgcaccagt tattgtcact
     4681 gtcggaatgg ctgacggcgt tgtttggcta ccgactaata gttcagactg cgcgatcaat
     4741 ttaccttcgg gaactgtggt caatgtttcg gcaggcggtg catgatgaat tcgatttttg
     4801 gaaacgactc aggtctaatc atcgccgtta aagtcttcgg tattttcgca attatggttg
     4861 tgatgaccct gctaactatt tgggcagaac gccgggtagt tggtcggatg caaatgcgta
     4921 ttggtccaaa ccgagttggc ccgtttgggt tacttcaatc tttaatggac gggattaagt
     4981 tagcgcttaa ggaagaaatt attcctaagg gtgtacacat ggccgtgtac gtgctggcgc
     5041 cggttatttc ggcaactgct gcattcttaa catttgcggt tatcccattt ggcccagaag
     5101 tttcgatttt cggacagctt actcctttgc agttaactga ttttccggtc tcagttcttt
     5161 atgttttagc tatcgcatca atcggtattt acggaatcgt gttggctggt tggtcatcag
     5221 gctctacata tccgttactc ggtggtctac gttcaacggc acaaatgatc agttacgaaa
     5281 tcgcaatggg cctttcgttt gtcgcagttt tcttatacgc atcatcaatg tcaacatctg
     5341 aaattgtcgc tgctcaagag ccactttggt atgcagtgct attactgcca tcttttgcaa
     5401 tttatgttgt ttcgatggtt ggtgaaacta accgcgcacc atttgactta cctgaagctg
     5461 aaggtgagtt agtgggtggt ttccataccg agtactcatc attaaagttt gcgcttttct
     5521 tcttggctga atacgtaaac atggtgactg tttcagcatt ggcaacaact ttgttccttg
     5581 gtggctggcg ggcaccatgg ccaatttctc tttgggatca agcaaatact ggctggtggc
     5641 cgatgctttg gttcttcgga aaattatttg cattcctgtt tatcttcatt tggttacgtg
     5701 gcacgttacc tcgcatgcgt tatgaccaat tcatgcgctt tggttggaaa gtcttaattc
     5761 cattctcgat cgcctggatt ctgattgtgg caactgctcg ctatttacgg tcagcaggtg
     5821 cggtcgatac tcaatcactg ttgtttgccg ctggcgttgt tttggtagtg ggtctaagta
     5881 tttcttacgc acttgaatta cgagcaaaga agattgctgc gcaagaagaa cttcgaacaa
     5941 cagaacttgc taataaacct tttgatccat tcgaaggtgg cttcccagtt ccgccacttc
     6001 ccggacaaga agcaattcca tcaagacaca aggctttatc tgttaccgct aaaggggagg
     6061 gaaactaatg gctgagttac cacaactgat tcgcggtttt ggagttacat tccgctcgat
     6121 gtttaagaaa gttacaactg agcagtatcc agaagagaag gataagtttc cacccaagcc
     6181 aagattccat ggtcggcacc aactaaaccg ccatccagat ggtttagaaa aatgtattgg
     6241 ctgtgaatta tgtgcttggg cttgtccggc tgacgcaatt tatgttgaag gtgcagaaaa
     6301 ctcagaagaa gaacgttttt ctccgggtga acgttatggt cgggtttacc aaatcaatta
     6361 cttacgctgc atactttgcg gactttgtat tgaagcatgt ccaactcgcg cgttaactat
     6421 gacaaatgag tacgaacttg ctgataattc gcgagcaaaa cttatttacg aaaagcaaga
     6481 tttactagcg ccaataatgc cgggtatgca ggcaccacct catgcaatgg cgccaggtac
     6541 tactgaaact gattactacc ttggaaatat caagggtgat tctgcaaggg ttgattcgta
     6601 gtgaatggtg aatcagtagt tttttgggtc accgctattt ttgctgttgc tggcgcaatt
     6661 ggcatggtgg tttctcgcaa agctgttcat agcgcgcttt gggttgcctt cacgatgatc
     6721 aacattgcaa ttttgtattt tgcattagag gcagcatttt tgggtgttac ccaaatcgtg
     6781 gtttataccg gcgcagtgat gatgcttttc ttattcgtgc tcatgatggt tggcgttgat
     6841 tcgtcagatt cacttctaga aacaattaaa ggacaacgct tagttgcggt acttttagtt
     6901 cttgcattcg cagcgatggt tgttggtggt attgcggcga ccggagtaat tacagctcgt
     6961 ggccttgctg gagccaataa cttcaacggt ggaaatgtcc aaggtctggc tgagttaatt
     7021 tttggacgtt atttattagc tttcgaagtt acatctgcgc ttttgattac tgcagcagtt
     7081 ggtgcaatgt tgttagcgca ccgcgagcgg attacgccac gtaagacaca ggcgcagttg
     7141 caacgcgaac gggtattagg tggtcatcca acaccacttc ctgcacctgg tgtacttgcc
     7201 acaaacaacg cagtaaatgt acctgcgcta ttacctgatg gaacacctgc acaaatttca
     7261 ctgcctacgc agtttactca gccagatgaa agtaatcgtc cggaggtgaa caaatgaacg
     7321 caaacaacta tttagtgctt gctgcaattt tgttctcaat cggagcattt ggtgttttag
     7381 ttagacgtaa cgcaatcatt gttttcatga gcgttgaaat gatgcttaac gcagctaatt
     7441 tagcttttgt tgcggcggcc agaattaccg gaacactaga tggtcaagta actgcgttct
     7501 tcgtgatggt tgttgctgca gctgaggtag ttgttggcct tgccatcatc atgacaattt
     7561 tccgtacgcg taaatctgct tcgatcgatg aagcaagtct gttgaagtat tagaggttta
     7621 ttgatgattg ctgcattaat tggactgccg ttattaggcg cagttgtcct attaataggt
     7681 ggacgtaaaa caaattcgtg gggtcactat tttgcggttc tgatgtccgg agcttctttt
     7741 gtccttggca cctcgctatt tgttcagatg cttggtcgtg gcgaagaaga gcgcgcaatt
     7801 aagcagcatc tatttgattg gatcgcggtc ggcttattcc aagtgccatt tggtttacag
     7861 cttgatcaac tatctattgc atttgtgctg ctgattaccg gagttggaac tttaattcac
     7921 atctactcaa tcggttacat ggaacatgat gaaaaccgcc gcaaattttt tgcatattta
     7981 aatttattcg tcgcagcaat gttgctttta gttctcgctg ataattacct tttgctttat
     8041 gttggctggg aaggcgtagg tctggcttcg tacctactta ttagtttctg gcaacataaa
     8101 tcaacggccg cagctgctgg caaaaaagca tttattgtta accgcgttgg tgacgttggt
     8161 ttgtctctag cgattatgtt gatgttcgtg actctaggca cagtaacttt tgatggcgtg
     8221 gcagaaaata ttgccaatgc gaatgacaca acagttacct ggattggttt agccctacta
     8281 cttgcagcct gcggcaagag tgcgcaattt cctttgcagt catggttatt ggacgcgatg
     8341 gaaggtccaa ctccggtttc agcacttatc cacgcggcaa caatggttac tgccggtgtt
     8401 tatttgattg ctagagcaca ttcaattttt gatttatcgc aaactgcaag aactgcagtg
     8461 gtgattatcg gtgcaatcac attgttactg ggtgcttata tcggaactgc caaagatgat
     8521 attaaaaaag gtttggctgg ctcgactatg agccaaatcg ggtacatgat tcttgctgca
     8581 ggtttaggcc cagccggcta cgtatttgca attttccact tattaactca cggtatgttc
     8641 aaagccggct tattcctcgg tgctggatca gtaatgcatg gaatgcatga tgacgtaaac
     8701 atgcgtcact atggcgcact tcgcagtgca atgaaaatta cttacgtcac atttggtttg
     8761 ggtttcttag ccatcatcgg tgttccacca tttgcgggtt tttggtcgaa agacgaaatt
     8821 atttatgtcg cattcggaca gaatttgatc atcggtgtgg cgactttaat cggcgctggt
     8881 attactgcgt tttatatgac gcgaatgatg accatgactt tctttggcga ggctcgttgg
     8941 cagccagaag ttcatccgca tgaatctgga aaagttatga caattccgat gataattttg
     9001 gcggtcggct cagttttcgg tggcttactt ttactttttg ttggcgacat agagcactgg
     9061 cttgagccgg ttgtgggtta cgcaaaatca gaaggtggaa tatcacataa cactctgatt
     9121 ccaatcacca tgtcagttgt cttagttggc gctggatatg cctggattaa atatggtcgc
     9181 cgaccagttc cagtagcagc tccaactgat gttagtttct taactcgcgc agctcgcgca
     9241 gatgcttatg gtgatgcgtt taacgaatca gtatttatgc gtcctggtca acacttaacc
     9301 cgcgcattaa cttattttga tgcaaaagga gttgatgggg tcgtgaacgg attggctgct
     9361 gcaattggcg gcctatcggc tcgggctcgg atgacccaat ctggattcac tcgttcgtac
     9421 gcgctttaca tgttggcagg tgctgcattg attgcattcg tcctactctt agtgaggctt
     9481 taactatgtt gttaacaact ctgatggcgc taccgctggt tggtgcgtta gtagtttttg
     9541 ctcttccagt aggtagggaa ttacttgcaa agcagattgc ctttgccgtt tcattagtaa
     9601 ctgcgttagg cgcgatctat cttgggctta atttagacaa ttcaaaatct ggtctgcagt
     9661 ttttggaaag ttacgaatgg attccagcat ttggcattaa ttggactgtt ggcgttgacg
     9721 gaatctccgc agtcttaatt gtgatggcca ctattttggt gccacttgtg atcatcgctg
     9781 gctggaatga tgtggagcgt ctcggtcaag gaaccgtaaa aggttatttt tcacttttct
     9841 tgctgttaga aacattcatg atcggtgtct ttgcggctac tgacttattc ctgttttatg
     9901 tgttcttcga agcgatgttg ataccggttt atttcttaat tggaagattt ggtggtccac
     9961 aacgcgccta cgctgcgatg aagttcttac tttattcact agttggtgga ctatttatgc
    10021 tcgcttcgct aattggcctt tatgttgctt cgggcagaga actaggtgag ggaaccttcg
    10081 atttcactgc acttgcaaat ttaaatcttg atccagctac gcaaaagata ctgttcctag
    10141 gtttcttttt tgcctttgcg gtgaaagcac caatggtgcc attccacacc tggctacctg
    10201 atgcggcagc acaagcgact ccagcgactt caaccttgtt agtcggcgta ctagataaag
    10261 ttggaacatt tggcatgatt cgctttttgt taccaatttt tcctgatgca agtaagtact
    10321 acgcacctgt aattattggt ctagccgtag ttagcatctt ctacggcgcc ttacttgcaa
    10381 tcggccaaac ggatttattg cggttggtgg cgttttcttc agtttcgcac ttcggcttca
    10441 ttgtgatggg tattttcgta atgacttcac agggattaag cggcgcgact ttgtacatgg
    10501 ttaaccatgg tttctcaact ggcgccctgt tcattgtggt tggcttttta ataagtcggc
    10561 ggggatctaa attcattgca gactttggtg gcgtaaataa agtagcccca ctattggcag
    10621 ggtttgtttt actttcgggt ttgtcttcgc tagcgctgcc aggtatgtcc agtttcgttt
    10681 ctgaattctt agttttagct ggaacatttg cctacaacaa gaccgttgca gtcatcgcaa
    10741 cattcggtat cgtacttgca gctttctaca tactttggat gtatcatcgc accatgacgg
    10801 gtgagccatc aaatgaggtt gtgcagacta tgactgagat caatcgtcgt gagttagctg
    10861 caattactcc acttattgcg ttgataatcg ccttaggttt tgcgccgcaa gttgccttga
    10921 atgtgataaa cccagcggtt gggcaggtcc aagattttgt tgatgtccaa gataaacaac
    10981 cagaaattaa atcaactgga ctggtcgaac tttcaatgaa ggttggtgcc taatgcaaat
    11041 ttcaattgag tatgcagctt tagcaccaat tctgattgtg cttggtgccg gcgttatttc
    11101 ggtattactg gaagctttta cgccacgtcc agctagacgg ccattgcaac tgatactagt
    11161 tttcggttcg ctaatgttgg catttgttta tgttgtagtg aattcatcag cacctcttac
    11221 tggacctgca gaagcagtag gcggtcagac taaaggaatt cgccagatta ttgctcaagg
    11281 ttccgtagtg cttgatggcc ctggattatt tattcaagga attttactca tcatctcttt
    11341 tattgcggcg ttgatgattg ctgagcgaag tattgattca cagggtgacg cgttcgcacc
    11401 acgtgcatca gccttacctg gcagtgaaga tgaacgcgct ttcactgcgc aaggcgttct
    11461 gcaaactgaa atctggccac tgtacttatt cagtgttggt ggaatgctac ttttcccagt
    11521 ttcaaatgac ttgctaacaa tgtttattgc tcttgaagtt ttttcactgc cactttattt
    11581 acttgccgga atggctagac ggcgtcgatt actcagtcaa gaagctgcac ttaaatactt
    11641 cgtgcttggc gcatttgcaa gtgctttctt tttatttggc gctgccttaa tttatgcagt
    11701 taccggtgtg atgcaatttg gccagttagc tgaggcatta gctaactcag gtcaagacgg
    11761 attagtgctt ggcggtatgg gattaatcgc tgtcgggtta cttttcaaaa ttggcgcagt
    11821 tccattccat caatggactc ccgatgttta ccaaggttca ccaactccaa tcactgcttt
    11881 tatgagtgca gcgaccaaaa taactgcttt tgctgcacta cttagagttt tctatgttgt
    11941 gtttggtggt ctgcgctggg attggcaacc agtgatgatg gttattgcgt tacttagtat
    12001 gattttcggc tcactacttg cagtaacgca aaccgacata aagcgtatgc ttgcttactc
    12061 atctgttgca cacgctggtt tcttgcttct tggcttaatc gcagcatcac caaatggttt
    12121 atcgagttca ctgttctatt tatttgctta tggacttacc tcgcttggtg cattcggtgt
    12181 ggtttcgcta gttcgtgatt catcgggcga agcaacacat ttaagtcagt gggctggtct
    12241 tggtaaaaca tcgccttgga tagctagtac tttcgcacta tttttactag ctctagccgg
    12301 aattccttta acaagtggat tcactggaaa gtttgcaatc ttcactgcag cggttgaaag
    12361 tggccaaact ccattagtca tcgttgcagt cgttgcaagt gcaatcgcag catttttcta
    12421 tgccagagtt atcgttttga tgttcttcac aaatgcacca gaagatgctc cgagtgttgt
    12481 ggtaccaagt ttgctaacta caatcgctat cggcgtaacg gcagcattca ctttactgtt
    12541 aggcgttgtg ccacaaccag tattggattt atgtgccaat gctggagtat ttttgcgcta
    12601 aggactgaaa ggatcgaagc gcattttcta attcgcagtt ttatctaaac agtgaaagaa
    12661 ttgctttgtg accgtattat tcccaacaaa tctagcttca cttgaagctg cgttagttgc
    12721 tgggttagcg gcagttgaaa aagaattacg tagtgaaatc aaatcggatt atgaattagc
    12781 cgatatcaca gctcgccatc ttgttgaagc tggcgggaaa agatttagac cactaatggt
    12841 tttgttagcc gcacaattcg gtgacccaac tgcaaaaggt gttgtgcctt ccgctgtggt
    12901 tgttgaatta actcatttag caacgcttta tcacgatgat gtgatggacg aagcaacagt
    12961 gcgccgcggc accgagtcag caaatagtcg gtggggtaat actgtggcta ttttgagtgg
    13021 cgacttttta tttgctcgct cttcgaagat attggcagac ttaggtccag aggctgtacg
    13081 aattcaagca caaacatttg aaagattagt aattgggcaa cttcgggaga ctaccggtcc
    13141 agcacctggc gaggatccag ttaagcacta cctagatgta ttggctggaa aaacaggatc
    13201 cttaattgcg gccgctggtc gctacggcgc gttaatgagc ggcgttaatc ccgagttgac
    13261 tgagcgaata gcacgttttg gtgaaaacat cggaatcgca ttccagttaa gtgatgattt
    13321 gttagacatc aaatcaaaca actcgggtaa aactccaggc aaagatttac gcgaaggcat
    13381 taaaaccctg ccagttttgt tcgcaattgc cgatccaaat acaacaccac aattaagaca
    13441 actactcagt ggtcctattg ctgatgataa agatcacgca ctggcactgg ctcaacttcg
    13501 cgaacacaga gcaatggatc aagctaacga tgtgttgctc gaatgggtag ctaaagcccg
    13561 cgaattactt agtgattttc cagagggtga accaaaagct gcgctacacg tgctctgcga
    13621 ttcgattgtg gatcgagata tttaaaataa atgaccaagc agcaacgcgg tgacgccgct
    13681 caaggagtcc tctacggatt tgctgcatac ataatctggg gtatcgtccc aatttattgg
    13741 ccactgctac agcctgccgg tgcgatagaa attttggcgc accgtgttat ttggagtctg
    13801 gtagtactcc ttgtcttttt ggctattaaa aagcggataa aaaaagcatt gcttattttt
    13861 aaagatccca aaaaattagc aattctgact attgcctctg tatttatcgc cgttaactgg
    13921 ggaatattta tttgggcgag tgtctccggt cgaatacttg actcatcatt gggttactac
    13981 ataaacccac tttttagtat tggccttggt gttttactgc ttaaagaaaa attgcgtaat
    14041 ttgcagtggt tggccatcgc aatcgcgact attgcaatta tttatttgtc ggttacttta
    14101 ggggcgccac cctatgtggc actttcgttg gctttcactt tttctattta tggctattta
    14161 aagaagcgag caaatgttga ggctatcgat agcctcacag ttgaaacctt gattctaatt
    14221 ccgttcgcca tcggttatat cgcttatttg tcatcggttg gtcagaattc atttggggtt
    14281 aatggaattg atcatgctat ttggttagcg agtgctggcg taatcaccgc agttccgctt
    14341 gcacttttcg gagcagctgc gattcgtatt ccactttcca cactcggctt tattcaatat
    14401 gttggcccaa ctctgcaatt cataattggg ctttatttgt ttaatgaacc aatgccacga
    14461 gatagattta tcggttttgc gctgacttgg gtagccattg caatcattag ttacgatgca
    14521 ttacgcaatc gaaataaagt tagtaaacag tacgtaccgg atctcgatta gttaacttgc
    14581 caagtttctt tgccaagtaa taatttagtg aggtctccag ggccggtttt cgcaataaca
    14641 tcatcaagtt gttcttgcat taatcgatcg taagaagccc ttttaacatc ccggaatact
    14701 ccaattggcg tatcttgaag tgtttctgga tgtgaaagtc ggcttaaacc aaaagctaaa
    14761 ctcggatctt catgatgagc atcgtgcaca actatttttg cttcgtccac acctacaacc
    14821 agagctactt ctaattcgcc gatttcattg cggatcacgc aacgatcgcg atttacacca
    14881 aagatgattt tttcaccatg ttcaagtcga attgtcacat catctttagt ttcagaattt
    14941 ttcaatggtt caaaagcacc gtcgttaaaa atatcgcaat tctgataaat ctcgataagt
    15001 gcagtgcctt cgtgggctgc agcagcgcga agtactgatt gtaagtgggc tcggtcgcta
    15061 tcaattgttc tagccacaaa agtggcttca gcgcctaggg caactgacaa cggattaaat
    15121 ggatagtcca atgatccaac tggcgtactt ttagttatct tcccaagtgg cgaagtaggc
    15181 gagtactgac ctttagttaa accataaatt cggttattga ataacaaaat ctttaaatta
    15241 acatttcggc gaagcgcatg gattaagtga tttccaccga ttgaaagtgc atcaccgtca
    15301 ccagttacca cccagaccga aaggtcaggg cgactagtag ctaagccggt cgcaattgct
    15361 ggcgcacgac catggattga gtgaacgcca taagtattca tgtaatacgg gaatcgactt
    15421 gaacaaccaa tgcctgaaac aaaaacaatg ttttcgcgct tcacaccaag ttctggcata
    15481 aaaccttgga ctgcagccag gatcgcataa tcaccacagc ctggacacca gcgcacctct
    15541 tggtctgttt tgaaatcttt agctgactgc ttttcgtcag tttttggcac tagcgcgagc
    15601 gatggaaatg tatcagacac ttgcaaccac atccttgatg gcatcaacca gttcttgagt
    15661 tttaaacggc agacctcgta cttggttata actgattgcg tcaactaaat acttcgctct
    15721 tatgagtaaa gcaagttggc ctaggttcat ttctggaatt attactttct cgtatttttt
    15781 caacacttca ccaagatttg ttgggaatgg attaacgtgc cgcaaatgtg cgtgtgcgat
    15841 atttaaacct tgtttgcgca cttcttcaca tgccgcattt actggaccat aagttgagcc
    15901 ccagccgata attaaaactt tcgcttgatt tgatggatca tctacagtta gcaggtcaat
    15961 tgactttgca atgccatcaa cttttgcttg acgagttcga accatgaagt cgtgattggt
    16021 tgggtcgtaa gaaatatttc cggtgccatc tgccttttca atgccaccaa ttcgatgttc
    16081 taatccagcc acaccaggga ctgcccaagg acgggcaagg gtttctggat cacgtttaaa
    16141 tggccaaaaa acatcagtgc catctggcag tttatggttt ggcccggttg caaatttagg
    16201 atcaatttcg attaagtcat cttcacttgg aactcgccaa ggctctgcac catttgcgat
    16261 gtagccatca gaaagcaaga aaaccggtgt gcggtaagta accgcaattc gacatgcttc
    16321 catagcagcg taaaagcaat cagagggaga ttgcggagca acgataggca ccggagcttc
    16381 gccattgcgc ccgaacatgg cctggagtaa atctgcttgt tcggttttcg tcggtaatcc
    16441 agtagatggg ccaccacgtt gaacatcgac gatgatcagc ggcaactcca acgcaacggc
    16501 taagccaatt gcctcactct ttagcgcaac accaggacca gaggtagttg tgacacctaa
    16561 catgccagca tacgaagcac caattgctgc accaatacca gctatttcat cttcagcctg
    16621 aaaagtgcga acaccaaaat ttttatgctt actcaattcg tgcaaaatgt cgcttgctgg
    16681 agtgatcggg taagacccca gaaaaattgt aagcttactt cgaaccgagg cggcaattaa
    16741 tccgtatgac aatgccaagt ttccactgat gtttcgataa gtgcctggag acattttggc
    16801 tggtttaact acatattgat tagcaaaact ttcagttgtt tcaccaaaag accaacctgc
    16861 ttgcagcgct ttttcatttg cagccaaaat ttgtggtttc ttgccaaact tcgtgcgtaa
    16921 aaactcaagt gttggctcga tcggacgaga gtaaagccaa agcaacatgc caagtgcaaa
    16981 catatttttg gcgcgttcag cttccttttt agtaatttca aactcagaaa gtgcctcaac
    17041 agtcattgaa gtcaatgcaa ttttgtgtac gttatagcca tcaagcgtgc catcttgaag
    17101 cggattagat ttgtagccaa cttttgaaat tgcccgatca ccaaattcgt cggtgtttac
    17161 gattaagtcg ccaccaagtc gcagctcttt taggttagct tttaaggcag ctggattcat
    17221 tgcaaccaac acatgagcct gatcccctgg cgtcatgatt gtgtggtcac caaaatttaa
    17281 ctgaaatgca gaaacgccag gaagcgttcc ggccggagct cgaatttcgg ctgggaaatc
    17341 aggcagagtt tttaaatcgt tcccaaaagc agctgtcgtg gaagtaaatt ggtcgccagt
    17401 taactgcata ccgtcacccg agtcaccggc aaatctaatg attacctgat cgagttctac
    17461 gacatctttc gacacttcgc cccagcccaa ttccttaagt tcagtaactc aattttcgca
    17521 tgaactcgat tagaacacct aacgggtatt ggccttaggg cgataccgat acatcaatgc
    17581 tttagagtga aaattgtgaa ttcacttgct gtgcacgtat tccaagccgg cttggcttgc
    17641 tgtggtgttg aagcggctgc cgtcgattta gttactcaag ccggagtgaa aattgtgccc
    17701 tctgatcaag acgcgaatat tctggtggtg gccggaacaa taaccaaagt tttagtacca
    17761 aaagtaatag ctgcctatga aaaactcgca gaaccaaaag tggtgattgc atttggagtt
    17821 tgcacgataa ctggaggacc ttactgggat tcttattcag ttatatctgg tgcccatgag
    17881 ttaatttcag tgaatataaa tgt
//
LOCUS       DBNHVN010000224         2699 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1527078, whole genome shotgun sequence.
ACCESSION   DBNHVN010000224 DBNHVN010000000
VERSION     DBNHVN010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1527078"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="AC16Y1_04270"
     CDS             complement(<1..1011)
                     /locus_tag="AC16Y1_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990391 - DNA repair complex [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrA family protein"
                     /protein_id="HIR5831710.1"
                     /translation="MVERLSVRGAREHNLKNVSLDLPRDSLIVFTGLSGSGKSSLAFD
                     TIFAEGQRRYVESLSAYARQFLGQMDKPDVDFIEGLSPAVSIDQKSTNRNPRSTVGTI
                     TEVYDYLRLLFARAGKPHCPDCGEPIFKQSPQQIVDRILEIKEGTKFQVLAPIIRERK
                     GEYVDLFRSLQSQGFARARVDGVVYTLEEVPKLKKQEKHTIEVVVDRLAVKPDSTRRI
                     TDSIETALRLGAGLVILDFVDLPENDPARERVFSEHLVCLNDGNQFEELEPRSFSFNS
                     PFGACPECTGLGSKKEVDPDLVIPDPTLSLNEGAIAPWASGHIIDYFLRLIKGLAKEL
                     DFD"
     gene            complement(1031..2020)
                     /locus_tag="AC16Y1_04275"
     CDS             complement(1031..2020)
                     /locus_tag="AC16Y1_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR5831711.1"
                     /translation="MDVSLNIWIGLVAAILILIVVDLLTVSRKPHDVMFKEATFWSVF
                     YIAIAIAFGIWVGREFGGQYGTEYFAAYLVEKSLSVDNLFVFVIILTQFAVPSIYHQR
                     VLMIGILLALVMRAIFIAIGAAALEAFAFTFVIFGAILIWTGVKLFQHWDEDPDMDEN
                     KMVKAVRRLVPVTDEYHGSKSFIKQNGKRVATPMFLVLIAIASTDLLFALDSIPATFG
                     VTSEPFLVFSANAFALLGLRALYFLLKGLLDKLVYLSLGLSFILIFIGFKLVFVYFHE
                     IYSWVPKISTNVSLVVIATILIVSTIASVIKTRRDPSAVAHAGRLTDPKNENE"
     gene            complement(2026..>2699)
                     /locus_tag="AC16Y1_04280"
     CDS             complement(2026..>2699)
                     /locus_tag="AC16Y1_04280"
                     /inference="COORDINATES: protein motif:HMM:NF015686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR5831712.1"
                     /translation="AAAIQRFAVTFYVFAAFLFYTAIHVWRSHDQEPDPEGNGLVRMA
                     EKVFPTVKQYQGAKLIVKRDGKRFITPMFLVILAIGTTDLLFALDSIPAILGLTKEPY
                     LVFMANAFALMGLRQLFFLLDGLLAKIIYLPKGLAIILGFISIKLLFEALHETTEIHV
                     PDISIGQSLSVIGSTLTLTVLASLLAVKRNPQLVRTSELAEAVEESIEHKGEALAHLK
                     DMDKA"
BASE COUNT          848 a          606 c          542 g          703 t
ORIGIN      
        1 atcaaaatct aattcttttg ctaaaccttt aatcagcctc aagaagtagt cgataatgtg
       61 accagatgcc caaggtgcaa tggcaccctc gttaagcgac aaggttggat ccgggataac
      121 taaatcagga tcaacttctt ttttactgcc aagacccgta cattcgggac atgctccaaa
      181 tggtgagttg aatgaaaacg aacgtggttc caattcttca aattgattgc catcgtttaa
      241 acaaactaga tgctcactga aaacacgttc ccgtgctgga tcattttcag gtaaatcaac
      301 aaaatctaaa attactaagc cagctccgag gcgcagtgcc gtttcaatac tgtcggtaat
      361 ccgtctagtt gaatctggtt tgactgcaag tcgatctacc acaacttcaa tagtgtgctt
      421 ttcctgcttt tttaatttag gaacttcttc gagggtataa accacaccat caacgcgggc
      481 cctggcaaaa ccttgtgatt gcaaactgcg aaacagatca acgtattcgc ctttgcgctc
      541 tcggataatt ggagctagaa cttggaattt agttccttct ttgatttcaa gtattcggtc
      601 aactatctgc tgtggcgatt gcttaaaaat aggttcgcca cagtccgggc aatgcggttt
      661 accggcccgg gcaaaaagta agcgcaagta atcgtaaact tcagtgatcg tgccgacggt
      721 tgagcgcgga tttcggttag ttgatttttg atctatcgat actgctggcg acaggccttc
      781 aataaaatca acatcgggtt tatccatttg acccaggaat tgacgggcat aggcagatag
      841 ggattcaaca taacggcgct gaccttcggc aaaaatagtg tcaaaagcta gtgaggattt
      901 tcctgatccc gaaaggccag taaaaacaat tagcgaatct ctaggtagat ccaggctgac
      961 attttttaaa ttgtgttccc gagcacctcg gacacttaag cgttcaacca cggatcaata
     1021 ctagttcagg ctactcattc tcgttttttg gatcagttaa ccgaccagca tgagcaactg
     1081 cagacggatc tctacgagtt ttaatcacgc tggcgatcgt tgagacaatc aaaattgttg
     1141 caataacaac caaactgaca ttggtagaga tcttcggtac ccaactatag atttcgtgga
     1201 agtatacgaa aactaacttg aaaccgataa agatcaaaat aaatgaaaga ccaagagata
     1261 agtaaacaag tttgtccaat agtcccttaa gtaagaagta caaggcacgc aagccaagta
     1321 gggcaaaagc attagctgag aaaaccaaga acggttcact tgtcacacca aaagtagctg
     1381 gaattgaatc aagtgcgaat aataaatctg tgctggcaat cgcaatcaac accaaaaaca
     1441 ttggcgttgc tacccgctta ccattttgct taataaacga cttactaccg tgatactcgt
     1501 cagttactgg aaccaaacgg cgcactgctt taaccatttt gttttcatcc atatcggggt
     1561 cttcatccca atgttggaag agtttgacac cggtccaaat caaaatcgca ccaaaaatta
     1621 cgaaggtgaa agcaaacgcc tcaagagctg cggcaccgat tgcgataaag atggctcgca
     1681 tcaccaaagc aagcagaata ccgatcatca gcactcgttg gtgataaata cttggcaccg
     1741 caaactgcgt caaaataatt acgaagacaa ataagttatc aacagataac gacttttcaa
     1801 ctagataggc cgcgaaatac tcagtgccat actgaccacc aaattctcgg cctacccaaa
     1861 taccaaatgc aattgcaatt gcaatataga agactgacca aaaagtggcc tctttgaaca
     1921 tcacatcgtg tggcttgcgg ctaaccgtta aaagatcaac cacaatcaga atcaaaatgg
     1981 ccgcgactaa gccaatccaa atatttaacg agacatccat cctagttacg ccttatccat
     2041 atctttcaag tgagctagtg cttcaccttt atgttcaatt gattcttcta ctgcttcggc
     2101 caattcagaa gttcgaacta gctgtgggtt ccgtttcaca gcgagcaggc ttgccaaaac
     2161 cgttaacgtt aaagttgaac ctatcacgga caggctttgg ccgatactta tgtccggcac
     2221 gtggatttca gttgtttcat gtagagcttc aaataaaagt tttatcgaaa taaaaccaag
     2281 aattatggct aaccctttag gtaagtaaat tattttggcc aatagaccat caagtaaaaa
     2341 gaacagctgc cgtagcccca tcaatgcaaa tgcattagcc atgaaaacta aatacggttc
     2401 tttagttaat cctaaaattg ctggaattga atcaagggca aaaagtaaat ctgttgttcc
     2461 gatcgccaga ataaccagaa acatcggtgt gatgaaacgc ttgccatcac gcttcacaat
     2521 tagtttggca ccttggtact gcttaaccgt tggaaatact ttctcagcca ttcgaactaa
     2581 gccgttacct tctggatctg gttcctgatc gtgtgagcgc caaacatgta ttgcggtgta
     2641 aaacaggaat gccgcaaaaa catagaaagt aacagcgaat ctttgaatag ctgctgcgc
//
LOCUS       DBNHVN010000225         2852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1751178, whole genome shotgun sequence.
ACCESSION   DBNHVN010000225 DBNHVN010000000
VERSION     DBNHVN010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1751178"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="AC16Y1_04285"
     CDS             complement(<1..941)
                     /locus_tag="AC16Y1_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HIR5831713.1"
                     /translation="MSVTREAVDAALATVIDPEIRRPITEIDMVKGVQIEGGKVTVGV
                     YLTISACPMQDKIVSGVKEAVAKVRDVTAVEVELDVMSPEQRTALREKLQGPTKEIRF
                     NQPGNTTRIYAIASGKGGVGKSSVTTNLAAAMAAQGLTVGILDADIYGHSIPDMLGIT
                     AAPHKVENMIMPPQGHGIRVMSMLPLKPQGRHEPVAMRGPMLHRYLESFLAEVWWGDL
                     DVLLLDLPPGTGDIAISTAHLLPQSELIIVTTPQSAAADVAIRAGMLAPQLNQRVAGV
                     IENMSAFACPHCGEPIDMFGVGGGQMVAEVLTKNVGQ"
     gene            complement(971..1492)
                     /locus_tag="AC16Y1_04290"
     CDS             complement(971..1492)
                     /locus_tag="AC16Y1_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016469591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HIR5831714.1"
                     /translation="MARKEQRSPRLDQPLVRSRSIRPTVDSEAAGRVSERVARFLGSW
                     RFLGWMTLSIIAWLVWNQLAPDNLRVDPFPFLFLTLALSLQASYAAPLILLAQNRQAD
                     RDRVQYQEDREQTQRLIADSDYLTREIAALRLALGEVATRDYLRSEIRSLLEEFNELL
                     KNDQGNSEEKTEK"
     gene            complement(1495..2748)
                     /locus_tag="AC16Y1_04295"
     CDS             complement(1495..2748)
                     /locus_tag="AC16Y1_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020136756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter MgtE N-terminal
                     domain-containing protein"
                     /protein_id="HIR5831715.1"
                     /translation="MVTTTQTRIYLARLAGIAVFDPAGDQVGKIRDVVVTARATGRAP
                     TVLGFVVEVAPRRRIFMPMSRINSIDANAVVTSGVVNLRRFERRAGEKLVIAELLDRK
                     VTVISTDKKATVQDIAMTQERTRDWIISRLFIRHPGTGFARRGETENLDWNEVSGLFL
                     ESAEQGVDSLLASIETMRPADLANLLHELPAKRRLELVSGLGLEKLADVFEELSEDDR
                     VEIVSAMGLDRAREVVEEMDPDDAADLLSELPPEQAEEVLEAMDQEEAEDLRRLLSYD
                     DFSAGGMMTTEPVILGPDSTIAEALARVRVPELSPALASQVYVVRPPLETPTGKYLGV
                     AHVQRLLREHPSNLVSSALDTTLEPIGPGAPLGQVARYFATYNLVALPVIDEDDHLIG
                     AVTIDDVIDHMLPEDWRDKELDEEG"
BASE COUNT          767 a          679 c          638 g          768 t
ORIGIN      
        1 tcctgcccaa cgtttttagt cagaacttca gccaccattt ggccgccccc aacaccaaac
       61 atgtcgattg gttcgccaca gtgcgggcaa gcaaaagctg acatgttttc gattacgcca
      121 gccactcgtt gatttagttg tggcgccaac ataccggctc gaattgcaac atcagctgct
      181 gctgattggg gggtggtgac aattattaat tcactttgtg gaagtaggtg ggctgtactg
      241 attgcgatgt caccagttcc tggcggtaaa tcaagtagca gtacatctaa atctccccac
      301 caaacttcag ctaagaatga ttcaaggtag cgatgtaaca tcggaccgcg cattgcaaca
      361 ggttcatgtc gaccctgagg tttaagtggc aacatcgaca taactcgaat gccatggcct
      421 tgcggtggca tgatcatgtt ttcgacttta tgaggcgctg ctgtgatgcc aagcatgtct
      481 ggaattgagt ggccataaat gtcagcatca agaattccaa cagttaaacc ttgtgctgcc
      541 attgccgcag ccaagtttgt ggtgaccgaa gatttaccga cgccgccctt acctgaggca
      601 attgcataaa ttctggtggt gttacctggt tgattgaaac gaatttcttt cgttgggcct
      661 tgaagttttt cccgaagtgc agttcgttgt tcaggactca ttacatcgag ttcaacttca
      721 actgcggtca catcgcgaac tttagctacc gcttctttga cgccactgac aattttgtct
      781 tgcattgggc aagcagaaat tgtcaggtaa acaccaactg tgacttttcc gccttcgatc
      841 tgtacgcctt taaccatgtc gatttccgtt atcggacgtc gaatctcggg gtcgataaca
      901 gtggctaatg cagcgtcaac tgcttcgcgg gttacgctca caaatactcc agttgtgttt
      961 gaaactaaat ttacttttca gtcttttctt cggaattgcc ttgatcattt ttaagtaact
     1021 cattgaattc ttccagaaga gaacgaattt cgcttcttaa gtaatcacgg gtagccactt
     1081 cacctaaggc aaggcgtaat gcggcgatct cacgagttaa ataatcagag tcagcaatta
     1141 agcgctgtgt ctgttcgcga tcttcttggt actgcacccg atcgcggtca gcttgacgat
     1201 tttgggcaag caaaatgagt ggcgcggcat aagaagcttg aagcgataat gccaaagtta
     1261 aaaaaaggaa tgggaatgga tcaactcgta agttatcagg agcaagttga ttccaaacca
     1321 accaagcaat gatagataaa gtcatccaac caagaaaacg ccaggagcca aggaagcgag
     1381 caactcgttc gcttacgcgg ccggctgctt cggaatcaac agttggtcga attgagcggc
     1441 ttcgaactaa tggctgatct aatcgtggac ttctttgttc tttgcgtgcc atgatcagcc
     1501 ctcctcatct aactctttat ctcgccagtc ttctggcaac atgtggtcga taacgtcatc
     1561 aatcgtcacc gccccgatca agtgatcatc ctcgtcaatc actggtagcg ccaccaagtt
     1621 gtaagtcgcg aagtagcgcg ctacttgacc aagcggagcg cctggaccaa ttggttcgag
     1681 tgttgtatca agagcactcg atactaaatt cgacggatgt tcacgcaaca aacgctgaac
     1741 atgtgccaca cctaaatatt ttcctgttgg agtttcaagt ggcggtctaa ctacgtaaac
     1801 ctgtgaggct aaagcagggg aaagttcagg aacccgtacc cgagctaatg cttcggcgat
     1861 tgttgaatca ggtccaagaa taactggttc agtggtcatc atgccacccg cggagaaatc
     1921 atcataagaa agtagtcgac gaagatcttc ggcttcttcc tgatccatcg cttcaagcac
     1981 ttcttcggct tgctcaggag gtagttcact taacaagtct gcagcgtcgt ctggatccat
     2041 ttcttcaact acttcgcgag cacggtcaag gcccattgca gatacgatct caactcgatc
     2101 atcttcagat aattcttcaa aaacgtcagc aagtttttct aaacctaaac cgcttactaa
     2161 ttccagacga cgtttagctg gcagttcatg taacaagttg gcaagatcgg ctgggcgcat
     2221 tgtttcaatc gaggcaagta gtgaatcaac accttgttct gcagactcaa gaaagagtcc
     2281 tgaaacttca ttccaatcta aattctctgt ttcgccgcgg cgagcaaaac cagtacctgg
     2341 atgacgaata aataggcgag agataatcca gtcgcgggta cgttcttgag tcatcgcaat
     2401 atcttggaca gttgcttttt tatcagtact tattacggtg actttgcgat ctaaaagttc
     2461 agcaataact aatttttcgc cagcgcgacg ttcaaatcga cgtaaattaa ccacaccgct
     2521 agttactacg gcatttgcat caatggaatt tattcgtgac attggcataa aaatacgacg
     2581 acgaggagca acttcaacaa caaagcctag gacagttggc gcacgtcctg ttgcacgagc
     2641 agtaactaca acatcgcgaa tcttgccgac ttgatcacct gctggatcaa atacggcaat
     2701 tccggctaac cgagccagat agatgcgagt ttgagttgta gtcaccatcc aaagttaccg
     2761 cagaaataag ccttttcttt gccaccaaat attcaagtgt agataattat tgactttaag
     2821 gctgttgaaa ttattcaaat aggctttatg cc
//
LOCUS       DBNHVN010000226         4144 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_265525, whole genome shotgun sequence.
ACCESSION   DBNHVN010000226 DBNHVN010000000
VERSION     DBNHVN010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_265525"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /gene="yaaA"
                     /locus_tag="AC16Y1_04300"
     CDS             <1..439
                     /gene="yaaA"
                     /locus_tag="AC16Y1_04300"
                     /inference="COORDINATES: protein motif:HMM:NF015820.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HIR5831716.1"
                     /translation="FGLISLGDRIPAYRLSADTKLPKIGTLANVWAKPCTEIFENQKG
                     LIVDLRSSQYVSLAPIPKSISNQVVMPRILQKMPSGPPKIVSHHNKATKGRILRQVVT
                     GAKIPTTADQLANVIAKLGADVEIVKPKKLGQSVTLNVIVKSL"
     gene            615..1808
                     /locus_tag="AC16Y1_04305"
     CDS             615..1808
                     /locus_tag="AC16Y1_04305"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR5831717.1"
                     /translation="MKLASRLENLGTETAFAVSLAAAEWGAKGNRIFPFHLGDLNLPT
                     SQNIVEAMNKAIATGKTGYCPGAGIPQLREALAANVGGDRGLNFGPENVVVQPGGKPV
                     ITKFIQTIMNPGDEVLYPNPGYPIYESQIEYFGGVAVPYRYVPTGTGFSIDLDQIKAS
                     ITPKTKAIVYNDLQNPNGAESTDEEREAIAEIAIKNNLYVLSDEAYFEMRYAGKSKSI
                     ATLPGMQERTVILYTYSKKFAMTGWRLGAAIAPKEIADQIAKLNTNDESCTTHFVQYA
                     GVEAITGDQSGVPKMLDTLKQRRDVAVVLLRQMPGVIVHTPEAGFYLFPDVTEAMQNK
                     GISDVTQFATEALHATGVSFTTRRHFGRPQPGEKEQYIRLAFSGIDASDISDGLKLMG
                     EWIAN"
     gene            1809..2771
                     /locus_tag="AC16Y1_04310"
     CDS             1809..2771
                     /locus_tag="AC16Y1_04310"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR5831718.1"
                     /translation="MARIVVTGKIPSGGLNRLKAEHDVVAWEKDEPISRKELLSMVSG
                     ADAIVSLLTEKIDDELLDAAGPQLKSVSNVAVGYNNIDVPACAKREVVVTNTPGVLTE
                     ATADIAMSLILMVTRRLSEGERVIRAKQPWQWGMFYMLGSAIQGRQLGVVGMGQIGIA
                     TARRAKAFGMNIAYTRRTPLEDHVVEELDAKYMSMDELIESSDVLSLHCPYSPATHQL
                     IAENQLARMKPTAYLINTARGPIVNEQHLVDALKAGKLAGAGLDVFEHEPAVNPGLLD
                     LDNVVLLPHLGSATIETRAAMADMAATNALAILAGNPAPNQVFA"
     gene            2817..3174
                     /gene="rnpB"
                     /locus_tag="AC16Y1_04315"
     ncRNA           2817..3174
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC16Y1_04315"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(3206..>4144)
                     /locus_tag="AC16Y1_04320"
     CDS             complement(3206..>4144)
                     /locus_tag="AC16Y1_04320"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5831719.1"
                     /translation="ALTSGLSSSTILVIAVIGAIFDPAGYTARKTLLADVAKASEIKL
                     DRLNGIHEGFMGVSWVFGPAVGAGLISIVGAVNSFWVAGGLFIFAALTIAFLRVGNLG
                     KDARDLAEEMGEKSDRSLRVGFQVLWGDKLLRTITISILIIAAVYLPTETVVLSTYFE
                     DLGQPVSLGIVISALAAGSAVGSFGYGWISARLSRKNLVRTTLVGTAVSILPMAFLPP
                     LPILIVAGFFLGLSWGPFNPLVSTLIQQRVPADQQGRVFGVQTAVFYAAPPLGMVLAG
                     LSVESFGVSTTYIILAAILSATALLALLTKSLRSNF"
BASE COUNT         1220 a          905 c          954 g         1065 t
ORIGIN      
        1 atttggactt atttcattag gtgaccgaat tccggcctat cgactatcag cagatactaa
       61 gttgccaaaa attggaactc tggcaaatgt ttgggctaag ccgtgtaccg aaatttttga
      121 aaatcaaaaa ggtttaattg ttgacttgcg ttcgagtcaa tatgtaagtc tggcgccaat
      181 tccaaaatcg atttcaaatc aagttgtgat gccaaggatt ttgcagaaaa tgccatctgg
      241 tccaccaaaa attgttagcc accacaataa agctacaaaa ggtcgaattc ttcgccaagt
      301 tgtgactgga gcaaaaattc caacgacggc tgatcagctg gcaaatgtta ttgccaaact
      361 aggcgcagat gtagaaatag ttaaaccgaa aaaactcggt cagtccgtaa ctttaaatgt
      421 gattgtaaaa agtctctagc aaattgtttc cgggtttcaa atagtgaaac ctgattaatt
      481 gccctgagaa gtaggctctg acaattgttt ttgatgtagt ggatccaatg tcgaatccaa
      541 aattgttgcc aattttattt ttatcagagc atcgataaag tactacctac atttagataa
      601 taaggatgtt ggccatgaaa ctagcgagcc gattagaaaa tcttggtacc gaaactgctt
      661 ttgctgtttc gcttgcggct gcagagtggg gtgcaaaagg aaatcgcatc tttccatttc
      721 accttggcga tttaaatctg ccaacatcgc aaaatattgt tgaggcaatg aataaagcga
      781 tcgcgacggg taagactggc tactgcccag gcgctggtat cccgcaactt cgtgaagccc
      841 tagcagcaaa tgtcggtggt gaccgtggat taaattttgg tccagaaaat gttgtagttc
      901 aaccaggtgg caaacctgta atcactaagt tcatccaaac aatcatgaat ccaggtgatg
      961 aagttctata tccaaacccc ggctatccga tctatgaaag ccagattgag tattttggtg
     1021 gagtagcagt tccatatcgt tatgtaccaa ctggaactgg attcagtatc gatttagatc
     1081 aaataaaagc ttcaattaca ccaaaaacta aagcaatcgt ttataacgac ttacagaatc
     1141 caaatggcgc agagtccact gatgaagaac gcgaagctat cgctgaaatc gctataaaga
     1201 ataatcttta cgtgctttcc gatgaagcat attttgaaat gcgctatgcc ggaaaatcaa
     1261 agtccattgc cacgcttccg ggcatgcaag agagaacggt aattctttat acctattcaa
     1321 aaaagtttgc gatgacaggt tggcgcttgg gtgcggcgat tgcaccaaaa gagattgctg
     1381 atcaaattgc caaactcaac accaatgatg aatcttgtac aactcacttc gttcagtatg
     1441 ccggtgttga agcaatcacg ggtgatcagt ccggtgtgcc aaagatgctc gatacgctaa
     1501 agcagcgccg agatgtggct gttgtcttac ttcgacaaat gccaggcgtt atagtacaca
     1561 cgcctgaagc aggtttctat ttattcccag atgtaacgga ggcaatgcaa aacaagggca
     1621 tcagcgatgt tacccagttt gccactgaag cacttcatgc aacaggtgtt tcctttacaa
     1681 cccgtcgcca cttcggtcga ccacaacctg gcgaaaaaga acagtacatt cgattagctt
     1741 tctcgggtat cgatgcaagt gacatttccg atggtttaaa acttatgggc gaatggatcg
     1801 ctaactaaat ggctcggatc gtcgtcaccg gaaaaattcc aagcggaggc cttaatcgtc
     1861 ttaaagccga gcatgacgta gtggcttggg aaaaagatga gccaatttct cgtaaagaac
     1921 tactttcgat ggtttctggt gctgatgcaa tcgtttcatt gctaaccgaa aaaattgatg
     1981 atgagctact cgatgctgcc ggcccgcaac ttaagtcagt ttctaatgtg gcagttggct
     2041 acaacaatat tgacgttcca gcctgtgcaa agcgtgaggt agtggtcacc aatactccag
     2101 gagttttaac tgaggcgact gcagatattg ccatgtcatt aatccttatg gtcactcgtc
     2161 gcctaagtga aggtgaacgg gttattcgag caaaacaacc atggcaatgg ggcatgttct
     2221 acatgcttgg ctccgcgatt caaggtcgtc aacttggcgt tgttggcatg ggtcagattg
     2281 gaattgcgac tgcccggcga gctaaagcat ttggcatgaa cattgcttac actcggcgca
     2341 cgccacttga agatcacgta gtagaagaat tagatgcaaa gtacatgtca atggacgagt
     2401 taattgaatc gagcgatgta ctttcactgc attgcccata ttcaccggct actcaccagt
     2461 taattgctga aaatcaacta gctcgcatga aaccaacggc ttatttgatt aatactgctc
     2521 gaggtcccat tgttaatgag cagcatttag tcgatgcgct caaggctgga aaactggctg
     2581 gtgctggcct ggatgtattt gaacatgaac ctgcagtgaa tccaggactt cttgatttgg
     2641 acaatgtcgt tttattgccg catttaggct cagccaccat cgaaacacgg gctgccatgg
     2701 cagacatggc cgcaacaaat gccttggcta ttttggccgg aaatcctgcg ccaaatcagg
     2761 tttttgctta attcacattt tcctttttat tcaggaagat ttatccttaa gccagacgag
     2821 ttggttggat gatcgcggat agaaatattc gaggaaagtc cggactccac agggcaacag
     2881 tggtggataa cgtccacttg gggtgaccca caggaaagtg caacagaaag taaaccgccc
     2941 aatttattgg gtaagggtga aacggtgagg taagagctca ccagtaattg tggtgacaca
     3001 gttagctagg taaaccccac tgggagcaag accaagagag ccgaaaggct tgagcaagta
     3061 gcggcccgct tttacttgca ggtaggtcgc atcaggttgt tggcaacaac aatcgcagat
     3121 ggatgatcat cgcgcgcaag cgtacagaat ccggcttaca gaccgactcg tctttatttt
     3181 attaacatag tcgtcaaacc tagccttaga agtttgaacg caatgacttc gttagaagtg
     3241 ccagcaatgc ggtcgctgag agtatcgcag ccaaaattat gtatgtagtg ctgactccaa
     3301 aactttcaac agatagtcca gctaaaacca tgccaagtgg aggggcggcg taaaagactg
     3361 cagtttggac accaaatact ctgccttgtt gatcagccgg aaccctttgt tgaatgagag
     3421 tgctcaccag aggattgaaa ggtccccacg ataaacctag aaaaaatcct gcaactatca
     3481 gaattggcaa tggtggcagg aaggccattg gaagaatgct tactgcagtt cccactaggg
     3541 ttgtccgtac aagattcttt cgtgagagtc gagcgcttat ccagccataa ccaaaggacc
     3601 caactgctga tcctgcagca agagccgaga taacaattcc aagactgaca ggttgaccta
     3661 aatcttcaaa gtaggtagat aagacaactg tctcggtcgg cagataaact gcagcaatga
     3721 ttaaaatcga aatagtaatt gtgcgaagca gtttgtcacc ccagagaact tgaaagccaa
     3781 ctcgaagact tctatctgac ttttcaccca tttcttcagc cagatctcgg gcatcttttc
     3841 cgagattgcc aactcgtaag aaagcaatag taagtgcagc aaaaatgaag aggccgccgg
     3901 cgacccagaa tgaattcact gcgccgacta ttgaaattaa gcctgctcca acagctgggc
     3961 caaaaaccca tgaaactccc atgaaccctt cgtggattcc attgagtcga tcaagtttaa
     4021 tctctgatgc ctttgcaaca tctgccaata acgtttttcg cgccgtgtaa cctgcgggat
     4081 caaagattgc accaattacg gcgataacta atattgtcga actcgatagt cctgatgtta
     4141 aagc
//
LOCUS       DBNHVN010000227         1224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_189557, whole genome shotgun sequence.
ACCESSION   DBNHVN010000227 DBNHVN010000000
VERSION     DBNHVN010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_189557"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..1006
                     /gene="trhO"
                     /locus_tag="AC16Y1_04325"
                     /gene_synonym="yceA"
     CDS             98..1006
                     /gene="trhO"
                     /locus_tag="AC16Y1_04325"
                     /gene_synonym="yceA"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent tRNA uridine(34) hydroxylase
                     TrhO"
                     /protein_id="HIR5831720.1"
                     /translation="MELEKILLYYGFTPLEDPAAVQLWQKTLCESLGLKGRILISKHG
                     INGTVGGNVEALKVYVKQTKKYPGFKKIDFKWSAGGAKDFPKLSVKVRDEIVTFGKPE
                     EIKVDRNGIIGGGKHLTPEQVNELVAARGQEVVFFDGRNAFEAKIGRFENAVIPNVET
                     THDFIAELESGKYDHLKDKPIVSYCTGGIRCEILSVLMKNRGFNEVYQIKGGIVRYGE
                     RFADKAHWKGSLYVFDKRMATNFSPDTEIIGECEKCSAKTNSFYNCSNLACRELTLLC
                     DPCAQTYSSESCIHQKSLSGATLHTS"
     gene            complement(980..>1224)
                     /locus_tag="AC16Y1_04330"
     CDS             complement(980..>1224)
                     /locus_tag="AC16Y1_04330"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR5831721.1"
                     /translation="RKRESLADASAVEYTRNPTGLRRALETLAKDSTVVRQKSNAVAH
                     MWIESPLDGKAVSRLFATHPPLAERIEILRRMESGA"
BASE COUNT          364 a          247 c          276 g          337 t
ORIGIN      
        1 agtcgacttt acttgtatga gacttttgaa agttcctgcc aaccaaaagc taaagcgatt
       61 taaccattaa cttgaatttg tttagaataa ataggtcatg gaactagaga aaattctcct
      121 ttactacgga ttcacaccgt tagaggatcc agcagcggtt cagttatggc aaaaaaccct
      181 ctgcgaaagt ttaggcctga agggtcgaat cttaatttca aagcatggaa taaacggcac
      241 agtcggtggc aatgttgaag cgttaaaggt ttatgttaag caaactaaaa aatacccagg
      301 atttaagaaa attgatttca agtggtctgc tggcggtgca aaagattttc ctaagttaag
      361 cgtcaaagtg cgagatgaga tcgtgacttt cggtaaaccc gaagaaatta aagtggatcg
      421 caacggaatt atcggtggtg gcaagcacct aacacctgaa caagtaaacg aattagtagc
      481 cgcccgtggt caagaagtcg ttttctttga tggccgaaat gcatttgaag caaaaattgg
      541 acgctttgaa aatgcagtga ttccaaacgt cgaaacaaca catgatttca tcgctgaatt
      601 agaaagtggc aaatacgacc acttaaaaga taaaccgatc gtttcttatt gcactggtgg
      661 aattcgctgc gaaatacttt ccgtattgat gaaaaaccga ggcttcaatg aggtttacca
      721 aattaaaggt ggcattgttc gttacggtga aagattcgcc gataaggcac attggaaagg
      781 ttcgctgtac gtgttcgata aacggatggc gactaacttt tcacccgaca ctgaaattat
      841 tggtgagtgc gaaaaatgta gcgccaagac gaacagcttc tataattgct cgaacttagc
      901 ttgccgggaa ctcacgctac tttgtgatcc ttgtgcgcag acttactcaa gtgaatcttg
      961 tatccaccaa aagtctcttt caggcgccac tctccatacg tcttaagatt tcgattcgtt
     1021 ccgccaacgg tggatgagtt gcaaaaagtc gcgaaactgc tttgccgtcc agtggtgatt
     1081 caatccacat gtgtgcgact gcatttgatt tttgcctgac aactgttgaa tcttttgcca
     1141 gtgtttctaa ggctcgacgt aatccggtcg gattacgggt gtactcaaca gcacttgcat
     1201 cagctagcga ttcacgtttt ctcg
//
LOCUS       DBNHVN010000228         1547 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1900152, whole genome shotgun sequence.
ACCESSION   DBNHVN010000228 DBNHVN010000000
VERSION     DBNHVN010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1900152"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1450)
                     /locus_tag="AC16Y1_04335"
     CDS             complement(<1..1450)
                     /locus_tag="AC16Y1_04335"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018537200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR5831722.1"
                     /translation="MTDSPAQLFAAAKARSALKKTLFGQFQAERPYELDQFQIDSCVH
                     LQNDKSVLVAAPTSAGKTIVGIFAVYLALSKQTRCFYTTPIKALSNQKFNEFVAEFGK
                     ENVGLLTGDNSINGDAPIVVMTTEVLRNMLYEGRNLNDLSHVVMDEVHYLADRSRGAV
                     WEEVIIHLPSHVKVVALSATVSNAEEFGDWLNTVRGETEIVVEEHRPTPLHQHVMVKD
                     GLIELFQNGEINRELLRIWQNEKSTEKFGRGRQRFYSRLTPSRIAVVEELERQGLLPS
                     ITFVFSRNGCNEAVNECLSAGLRLTSKEEQLEIRGIVDLIFADAQIDELQALGYAAWR
                     EGLERGIAAHHAGMLPAFKEVVEQLFAEGLVKVVFATETLALGINMPAKSVVLEKLVK
                     WNGSVHAPITPGEYTQLTGRAGRRGIDIEGHAIVIWHKELEPGNLAGLASTRTYPLKS
                     SFRPSYNMAVNLISRFGSSKSKDLLESSFAQFQ"
BASE COUNT          419 a          364 c          307 g          457 t
ORIGIN      
        1 cctgaaattg agcaaaacta gattccagta gatcttttga tttactggaa ccaaagcgtg
       61 aaattaaatt cacggccatg ttgtaactcg gacgaaagct tgacttcagc ggataggtac
      121 gagtacttgc taaaccagct aaatttcctg gttcaagttc tttgtgccag ataacaatcg
      181 catggccttc tatgtcaatc ccacgtcggc cggctcttcc agttagttgg gtgtactcac
      241 ctggagtaat aggtgcatgc acactgccgt tccacttaac taacttttcg agcaccacgc
      301 tcttggctgg catgttgata ccaagtgcca aggtttcagt agcaaaaacg actttgacta
      361 gaccttccgc aaatagctgt tcaactactt ctttaaaggc tggcaacatt ccagcatggt
      421 gcgctgctat gccacgctct agaccttcgc gccatgcggc atatccaagt gcctggagtt
      481 catcgatttg agcgtcagca aaaatgagat caacaattcc acgaatctca agttgttcct
      541 ctttgctggt caaccgaagt ccagcggaaa gacattcgtt cactgcttca ttgcatccgt
      601 tgcgagaaaa aacgaaggtt atcgaaggca atagtccttg acgctctaac tcttcgacaa
      661 ccgctatccg actaggagtt aaccggctgt agaagcgttg tctacctctg ccaaactttt
      721 ccgtggattt ctcattttgc caaattcgta aaagttctcg gtttatctca ccgttttgaa
      781 aaagttcaat tagcccatct ttaaccatta cgtgttgatg tagcggagtt ggtcggtgtt
      841 cctcaacaac gatttcggtt tcacctcgta cggtattcag ccaatcgcca aattcttcag
      901 cattactcac tgtcgctgaa agggctacca cttttacatg cgaaggtagg tgaattatta
      961 cttcttccca aacagctccc cttgatcgat cggcaagata atgtacctca tccatcacaa
     1021 cgtgcgataa atcatttaaa ttacggcctt cataaagcat gtttcgaagt acctcagtag
     1081 tcataaccac tattggtgca tccccgttaa tactgttatc cccggtcaat agaccaacat
     1141 tttccttgcc aaactcggcg acaaattcgt tgaacttctg atttgataat gcttttatgg
     1201 gcgtagtgta aaaacatctg gtttgtttac ttaatgccaa ataaactgca aaaattccaa
     1261 ctattgtttt acccgcactt gttggtgcag caactaaaac acttttgtca ttttgtaaat
     1321 gcacacatga atcaatttga aattgatcaa gttcatatgg acgctctgct tgaaattgac
     1381 caaagagtgt ttttttaagt gctgatctgg ctttcgctgc agcaaaaagt tgcgcaggag
     1441 aatcagtcat acctaaagat tatttgtttt tggtagcaga agtcggattc ggcttggtaa
     1501 aacactgaat gtggctggtc caaaacccat gcgttcacca tcagcgt
//
LOCUS       DBNHVN010000229         2113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1677289, whole genome shotgun sequence.
ACCESSION   DBNHVN010000229 DBNHVN010000000
VERSION     DBNHVN010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1677289"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="AC16Y1_04340"
     CDS             <1..485
                     /locus_tag="AC16Y1_04340"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR5831723.1"
                     /translation="FAGDISPFIVALDGVTDPRNLGAVIRSSAAFGADGVLVPERRAA
                     GMTASAWKTSAGAATRVPVARAVNLTRELVAYQKAGCTVFGLDADGDVNLHELDAALV
                     SGPIVLVVGGEATGMSRLVRDTCDHIISIPMFDESESLNAGVAAGVAMYEISKLRSIG
                     "
     gene            complement(486..1787)
                     /locus_tag="AC16Y1_04345"
     CDS             complement(486..1787)
                     /locus_tag="AC16Y1_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013115919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HIR5831724.1"
                     /translation="MALQVMAAPNEPDLLFLPWSHLLEQWPDEATVSLPKGISRHIVK
                     FVRLNGVVYAIKETQHPIAQREYELLQRLVRRGVPCVEPVAVISGREDRDGAELPAAL
                     MTRHLEFSLPYRALFSSTLKPDTAIRLLDALALLLVRLHLVGFSWGDCSLSNTLFRRD
                     AGAFAAYLVDAETGNLEGKLSDGQRDYDVDLAVTNVTGELMDLQVAGLLHESIDPIVT
                     GEQIRARYQKLWTAITSPLVMAPSERYKLDERVRKLNDLGFDVAEMMATTPLHPSDDE
                     IRIVMTPKVVDAGHHARRLIRLTGLDVEENQARRLLNDLDQYRLEIGLKAEDEELAAH
                     RWVTQVFEPVTNAVPKNMRGKLEPAELYHEVLQHRWFMSEKAGKDLGRDVAVDDYIKK
                     ILVNKPEEKAIIGSKLGSELEDTQEMRIVFPADMEDIDEVN"
     gene            complement(1819..>2113)
                     /locus_tag="AC16Y1_04350"
     CDS             complement(1819..>2113)
                     /locus_tag="AC16Y1_04350"
                     /inference="COORDINATES: protein motif:HMM:NF019998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HIR5831725.1"
                     /translation="AALTAAKAEGLSEITVGFRPEDLVEDANGFALTIDLVEVLGADA
                     YVYGTVKDAAGSERQITVRVDGRNTPAKGSTIKLSPTAGHLHVFSTRTGYRIG"
BASE COUNT          552 a          505 c          470 g          586 t
ORIGIN      
        1 cattcgctgg cgacatttca ccatttatcg tggctctaga tggcgtgacc gatccgcgta
       61 acttaggtgc ggtaattaga tcaagtgccg cctttggcgc tgacggggtt ttagttccag
      121 aacgcagagc agccggcatg accgcatctg cttggaaaac ttcagctggc gctgcaactc
      181 gggtaccagt tgctagagcc gtgaatttaa cccgcgagtt agtggcttat caaaaagctg
      241 gctgcacagt atttggtctt gatgctgatg gtgatgtgaa cttgcacgaa ctcgatgcag
      301 cgctagttag cggccctatc gtgttagttg ttggtggcga agcaactgga atgtcacgtt
      361 tagtacgcga tacctgtgat cacattattt caatcccaat gtttgatgaa tctgaatcgc
      421 ttaatgctgg agttgcggcc ggcgttgcaa tgtatgaaat ttctaaactc cgaagtatcg
      481 gatagttaat taacttcgtc gatatcttcc atatcggccg gaaaaacaat tcgcatttct
      541 tgagtgtctt ccaactcaga accaagtttt gatccgatga ttgctttttc ctcaggttta
      601 ttaaccaaaa ttttcttaat gtaatcatct actgccacat cgcgacccag gtctttgcca
      661 gctttttctg acatgaacca acggtgttgt agtacttcgt gatacaactc agctggctct
      721 aacttgccac gcatattttt tggcactgca ttagtaactg gctcaaaaac ttgagttacc
      781 cagcgatgag ccgctaactc ttcgtcttcg gctttcaagc caatctcaag gcggtactga
      841 tctaaatcat ttagcaggcg acgtgcttga ttttcttcaa catcaagacc agtcaatcta
      901 attaagcggc gagcgtgatg gccagcatct acaacttttg gggtcatcac aattcggatt
      961 tcatcatcac ttggatgtag cggagtagtt gccatcatct cagcaacgtc aaaacctaga
     1021 tcattaagtt tgcggactcg ctcatctagt ttgtatcgct cacttggggc cataactaat
     1081 ggactcgtaa tcgcagtcca cagtttttga taccgcgctc taatttgttc accagtgaca
     1141 atcgggtcga ttgattcgtg tagtaaccca gcaacttgta agtccattaa ttcaccggtc
     1201 acattcgtaa cggctaaatc aacgtcgtag tcacgttgac catcagaaag tttgccctct
     1261 aggtttccag tttcggcgtc aactaagtaa gccgcaaatg ctccggcatc gcggcggaat
     1321 aaagtatttg aaagtgaaca gtcaccccaa ctaaaaccaa ctaaatgcaa ccgaacaagt
     1381 agtaacgcca atgcatcgag cagacgaatt gcggtatctg gttttagcgt tgaggaaaat
     1441 aaagcacggt aaggcagcga gaactctaag tgccgagtca tcaacgcagc tggcagctcg
     1501 gcgccatctc gatcttcgcg gccggaaata acagccactg gttcaacaca cggcacgcca
     1561 cggcgaacaa ggcgttgcag taactcgtat tcacgctgcg caattggatg ctgtgtttct
     1621 ttgatcgcat aaaccacacc attaagacga acaaacttaa caatgtgtcg gcttatacct
     1681 tttggtagcg agaccgttgc ttcatctggc cattgttcaa gcagatgact ccatggcaag
     1741 aacaataagt caggctcgtt cggagccgcc attacctgta atgccattag taacctgcgg
     1801 ggtagttaat ttttttaatt aacctatgcg gtaaccagtg cgagtactaa atacgtgtaa
     1861 gtgaccagca gttggactca acttaattgt tgaacctttg gctggggtat tacgaccatc
     1921 tactcgaact gtaatttgtc gctcagaacc agcagcatct tttactgtgc cataaacata
     1981 tgcatcagca ccaagtactt cgactaagtc gattgtcaaa gcaaaaccat ttgcatcttc
     2041 gactaaatct tctggacgga agccaactgt gatttcactc agaccttctg cttttgcagc
     2101 tgttagtgca gcg
//
LOCUS       DBNHVN010000230         2800 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1974275, whole genome shotgun sequence.
ACCESSION   DBNHVN010000230 DBNHVN010000000
VERSION     DBNHVN010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1974275"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="AC16Y1_04355"
     CDS             complement(<1..267)
                     /locus_tag="AC16Y1_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831726.1"
                     /translation="MSKLNKVALVIVAGLALGACSNSENAQPQPTVSSKWSAAQAGYL
                     QEIYAANLAANDVFSEEIYIEIGNTMCQSFNDGRQSDELLTLLAA"
     gene            complement(294..2291)
                     /locus_tag="AC16Y1_04360"
     CDS             complement(294..2291)
                     /locus_tag="AC16Y1_04360"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIR5831727.1"
                     /translation="MSQNPSVEQALAAVVTEIGGSPRAGQIEMANAVAQSFSTKKHLL
                     VEAGTGTGKSLGYIVPALVRALDGVKADGRNRTIIATATLALQRQLIESDLPAATKAF
                     AKETGKEISFAVLKGRHNYICLDRFHRGPTDPDQDQDATLFQESKSTLGRQAKKLRKW
                     VEQTDTGDRDDYQDEIDPRIWRSVSISGRECVGAGKCPWGQECFAEEAKAKAFAADVV
                     VTNHALLAIHTIENHPILPDHDAIVIDEGHELVDRTTTALANSLDHRDVGRAAGMAKR
                     FISNKIHDKLVELGDDLDDVLSNLATTGTMSRLDELPPALSAILKNLKEQTKSAITEL
                     GSSSQDDLDEAATKHRARSALKEVFDTTSEILATDEHSVTWLDLARTATLHHAPLSVA
                     GFLSESLFAEQTVVMTSATLQVSGSMNATAKAVGLAENTDWTGLDVGSPFDYAKQGIL
                     YCAAHLPAPTSSGVAEEALDELADLIEAAGGRTLSLFSSWRAVERAEEYLKLRFRDRE
                     DRPLIVARKGDSVGELVRQFKQTAQSSLLGTVSLWQGIDVPGDTCTLVTIDRIPFPRP
                     DDPIFAARSARADESGSSGFRSVSLPRAALLLAQGVGRLIRSNEDRGVVAILDSRLAT
                     ANYGSILRKSIPPLWWTIDKDSVISALQRLDEQALSNAKKN"
     gene            complement(2288..>2800)
                     /locus_tag="AC16Y1_04365"
     CDS             complement(2288..>2800)
                     /locus_tag="AC16Y1_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR5831728.1"
                     /translation="EFTLSDTVGFVRDLPHQLVEAFRSTLEEVANSDLILHVVDGSDP
                     DPEGQLSAVRAVLAQIEATNVPEIVVINKCDAADPFVVDRIKLLEKNAIAVSALTGQG
                     IAELIEMIENQLPSPQIDVKVLIPYTQGDLLARVYKEGQLLSRTDTEHGYLVVAKVSQ
                     GLATQLEQLN"
BASE COUNT          719 a          692 c          608 g          781 t
ORIGIN      
        1 tgcagccaat agggtcagta actcgtcaga ttgtcggcca tcattgaagc tttggcacat
       61 agtgttacca atttcaatgt aaatctcttc agaaaaaaca tcattagctg ccaagttggc
      121 tgcgtaaatt tcttgcagat agccagcctg ggctgcgctc cacttcgatg aaacagtagg
      181 ttgcggctgg gcattttcag aattactgca tgcgccaaga gctaaaccag caacaataac
      241 tagtgcaact ttatttaact tcgacacgta aacctcgttt cacctaaaac ttatcagttc
      301 tttttggcat tacttaatgc ctgttcatcg agcctttgca acgcagaaat tactgaatcc
      361 ttatcaatcg tccaccaaag cggtggaatt gatttgcgca aaattgaacc atagtttgca
      421 gttgccaatc gactatcgag tattgctact actccacgat cttcgttact gcgaattagg
      481 cggccaactc cttgagcaag tagcaacgca gctcttggta gtgacacgga tcgaaatcct
      541 gaacttcctg attcgtcggc tcgggcactg cgcgcggcaa aaattggatc atccggacgc
      601 ggaaaaggga tgcggtcaat tgtgacaagt gtgcaggtgt cacctggcac atcaattccc
      661 tgccaaagtg aaactgtgcc taggagtgaa gattgcgcag tttgtttaaa ctgtcgtact
      721 agttcgccta ctgaatcgcc tttgcgcgcg acaatcaagg gtcgatcctc gcgatcccta
      781 aatctaagtt tcagatattc ttcggctcgc tcaacggcgc gccaagatga gaacaggctc
      841 agcgtccgac ctccagctgc ctcaatcaaa tctgctagtt catctaatgc ttcctctgct
      901 accccactcg aggttggggc tggcaaatgc gcagcgcagt aaaggattcc ctgtttggca
      961 taatcaaatg ggctgccaac atctagtcca gtccagtcag tattttctgc caaaccgact
     1021 gcttttgcgg tcgcattcat tgagccagaa acttgcagtg tggcactagt catcaccact
     1081 gtttgttcgg cgaataaaga ttcacttaag aatccagcca ccgagagcgg tgcatggtgc
     1141 aatgttgcgg ttcgagccag atcaagccaa gtgacactat gttcatcggt agccaagatt
     1201 tcacttgtcg tatcaaagac ttctttaagt gcagacctag cccgatgttt agtagcagcc
     1261 tcatccaaat catcttgtga agacgaccct aattcggtga tagctgattt tgtttgttct
     1321 ttaaggttct tcaagatcgc cgataaggcc ggcggtagtt catcaagtcg actcatagtt
     1381 ccagtagtcg ccaagttact aagtacatcg tctagatcgt caccaagctc tactaatttg
     1441 tcatgaattt tgttactaat aaatcttttc gccataccgg ctgctctacc gacatcacga
     1501 tgatcaagtg agttcgctag tgcagttgta gttcgatcaa ctaactcgtg tccttcgtcg
     1561 atcacaattg catcgtggtc gggcaaaatc ggatgatttt ctattgtgtg aattgcaagt
     1621 aaagcatggt ttgttacaac gacgtcagcg gcaaacgctt ttgctttagc ttcttcggca
     1681 aaacattctt gtccccaagg gcatttaccg gcaccaacac attcacgtcc agagatactg
     1741 actgagcgcc atatacgcgg gtcgatttca tcttggtaat catcacgatc accagtgtct
     1801 gtttgctcaa cccacttgcg aagtttcttt gcttgtcggc caagtgtgga ctttgattcc
     1861 tggaaaagtg ttgcgtcttg atcctgatca gggtcagtcg gtccacgatg gaagcgatca
     1921 aggcagatgt agttgtgccg acctttcaaa acggcaaagg aaatttcttt tccggtctct
     1981 ttggcaaaag ctttagttgc cgccggtaaa tcagactcga ttagttggcg ctgaagtgcc
     2041 aaggtggcag tggcgataat tgtgcgattt cttccgtctg ctttaacgcc gtcaagtgcc
     2101 cgaactaacg ctggaacaat gtatcccagt gacttacccg taccagttcc tgcctcgact
     2161 aacagatgtt ttttggtact aaaagattga gccaccgcat tagccatctc aatttggccg
     2221 gcgcgcggtg aaccaccaat ttctgtaacg accgcagcta gggcttgttc aaccgatgga
     2281 ttttgactca atttagttgt tccaattggg tagccaaacc ctgtgaaacc ttcgccacaa
     2341 ctaaataacc atgctcagta tcagttctgc ttaacaactg accttcttta taaactcgag
     2401 ctagtaaatc gccttgagta tatggaatta acaccttcac atcgatttgc ggggaaggta
     2461 actgattttc aatcatttca attagctcag cgatcccctg cccagtcagc gcagacaccg
     2521 cgattgcatt tttctcaaga agttttattc ggtcaaccac aaatggatcc gccgcatcac
     2581 atttatttat gaccacaatt tctggcacat tagttgcctc aatttgcgca aggactgctc
     2641 gaactgccga taattgacct tcgggatctg ggtcagatcc gtcaactacg tgcaaaatta
     2701 aatcgctatt tgcaacttct tccaaagttg atcgaaatgc ctccactaat tggtgcggta
     2761 aatccctaac aaaaccaacc gtgtcagaca gggtaaattc
//
LOCUS       DBNHVN010000231         1360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1380680, whole genome shotgun sequence.
ACCESSION   DBNHVN010000231 DBNHVN010000000
VERSION     DBNHVN010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1380680"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="AC16Y1_04370"
     CDS             <1..626
                     /locus_tag="AC16Y1_04370"
                     /inference="COORDINATES: protein motif:HMM:NF037653.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5831729.1"
                     /translation="WPLCVIVGPVPSSQVATEVHIEQIKRTAKLQKVEAMVGVQTDRL
                     ITIIGGENLSADTMSIAKNFVGHFGQGTVVVGPLANNLLAAATSAIAAISAYRSVALI
                     NQPPRLISADELVSARVINGDYSAIEPLVEKMQKLLKPDVLGTLATYLEEQPSIEGCA
                     RAQFIHVNTVRYRLKKVQDILGYDPTDAGAALVLRMALMLGRKKDTL"
     gene            706..>1360
                     /locus_tag="AC16Y1_04375"
     CDS             706..>1360
                     /locus_tag="AC16Y1_04375"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009775232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR5831730.1"
                     /translation="MLAIVAPGQGSQTPGFLTPWLELPNFEDRLRWLGTVAGIDLVKH
                     GTHSDEETIKDTAIAQPLIVASGLVSLLSLFPHPGESFSKVALGAGHSVGEVTAAAGA
                     HVMTAEQAMVFVRERGKAMADSAAITPTGMTAILGGERSAVIANLTKHGLTAANENGG
                     GQIVAAGTLEQLAALQADPPAGARIVPLSVAGAFHTIHMVPAAKTLGQHAKAISTHDA
                     "
BASE COUNT          359 a          310 c          326 g          365 t
ORIGIN      
        1 attggcccct ttgtgtgatt gttggtccag ttccatcaag tcaggttgca actgaagttc
       61 acattgaaca aattaaacgc acggcgaagt tgcaaaaagt cgaagcaatg gttggggtgc
      121 aaactgatcg acttatcaca attattggtg gtgagaattt atctgcagac acaatgtcga
      181 tagcaaaaaa ttttgtgggt cactttggtc aaggaaccgt tgtagttggg ccgttggcta
      241 ataacctttt ggctgcggca acatcagcta ttgcagcaat ttcggcctac cggtctgttg
      301 cgttgattaa tcaaccccca cggttaattt ctgcagacga gttagtttca gccagagtga
      361 ttaacggtga ctacagtgcg atcgaaccgc tggttgaaaa aatgcaaaag ttacttaagc
      421 cagatgtgct tggtacctta gctacctacc tcgaagagca accatcgatc gaaggctgcg
      481 cgcgagctca attcattcat gtgaatacag tgcgctaccg acttaaaaaa gtgcaagaca
      541 tccttggcta tgatccaact gatgcgggtg ctgcattagt ccttcgaatg gccttaatgt
      601 taggccgaaa aaaggacact ttgtagtatt catacaagaa atagccctag aatttagtta
      661 ttctcaagcc cttttgggtg ttacctatac ggaagactaa ttctcgtgct tgcaattgtt
      721 gctccaggtc agggttcgca aactccaggt tttcttacac cttggttaga gctaccaaat
      781 tttgaagatc gcttgcgttg gcttggcacc gtggctggaa tcgacttagt taagcacggc
      841 acccacagtg atgaagaaac aatcaaagac acagcaattg cccaaccact aattgttgcc
      901 tcgggtttag tttcattact cagcttgttt ccacatccag gtgagtcttt tagcaaagtt
      961 gcccttggtg ctggccatag cgttggcgaa gttactgccg ctgccggtgc tcacgtaatg
     1021 actgcggaac aagccatggt gtttgtgcgc gaacgtggca aagcaatggc tgattcagcg
     1081 gcaataactc ctacaggtat gaccgcaata cttggtggcg aacgttctgc cgtaattgca
     1141 aacttaacca agcatggatt aactgccgca aacgaaaatg gtggtggcca gattgttgca
     1201 gctggaactc ttgaacaatt ggctgcattg caggctgatc ccccagccgg agctcgaatt
     1261 gttccacttt cagttgctgg agcgtttcac acaatccaca tggttcctgc ggcaaaaaca
     1321 ttaggtcagc acgcgaaagc aatctctaca catgatgctc
//
LOCUS       DBNHVN010000232         1902 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_563148, whole genome shotgun sequence.
ACCESSION   DBNHVN010000232 DBNHVN010000000
VERSION     DBNHVN010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_563148"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /gene="argH"
                     /locus_tag="AC16Y1_04380"
     CDS             <1..909
                     /gene="argH"
                     /locus_tag="AC16Y1_04380"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR5831731.1"
                     /translation="LAKHVHALGRDLDRLQDWDKRTAKSPMGSGALAGSSIKLDPLAV
                     AQELGFDGVVENSIDGVSDRDFVAEFLFVIAMIGVHLSRLGEEICLWSSREFAWAKLD
                     DAWSTGSSIMPQKKNPDVAELARGKSGRLIGHVTSILTVLKGLPFAYNRDLQEDKESV
                     IDAIETMSLILPAMTGTVATLEFDTDRMLASAPEGFALATDIAEWVVKQGVPFREAHE
                     IAGACVRKAEQLGTDLNGLSDDDFQSIHRSLTSEVRSVLTVQGSINSRNTVNGTAPDA
                     VRRQIEVAKDKVALLTKWSTNLPHKV"
     gene            complement(906..1166)
                     /locus_tag="AC16Y1_04385"
     CDS             complement(906..1166)
                     /locus_tag="AC16Y1_04385"
                     /inference="COORDINATES: protein motif:HMM:NF016876.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE family
                     toxin"
                     /protein_id="HIR5831732.1"
                     /translation="MTYQVLISKKALKNLQKLSAFDNASVRKILTALSINPRPRNSIK
                     LANSDKYRVRVGNFRIIYTITDSELVVDVIDIDHRRKVYENL"
     gene            complement(1163..1420)
                     /locus_tag="AC16Y1_04390"
     CDS             complement(1163..1420)
                     /locus_tag="AC16Y1_04390"
                     /inference="COORDINATES: protein motif:HMM:NF014643.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR5831733.1"
                     /translation="MATYSISEARENLADAVTRSAVEEVFLEKHGKVVAVMISAEVYE
                     KLVDAWEEIQDLKMLEELEESGELDVPGIPMEQVFRELGLS"
     gene            1511..>1902
                     /gene="tyrS"
                     /locus_tag="AC16Y1_04395"
     CDS             1511..>1902
                     /gene="tyrS"
                     /locus_tag="AC16Y1_04395"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR5831734.1"
                     /translation="MTDILAELGWRNLVSQHTPELPEELAKGSVTLYCGFDPTAPSLH
                     MGNLAQIITMSRFQKLGHTPIALVGGATGSIGDPSGKNAERTLNTTDTVGEWVERLRK
                     QLEPFFEFSGKNAAIMSNNYIWTHNMDVL"
BASE COUNT          526 a          444 c          421 g          511 t
ORIGIN      
        1 ttagccaagc atgtgcacgc acttggccgc gatctagatc gacttcaaga ttgggataag
       61 cgcactgcca agtcaccgat gggtagtggc gcattagctg gctcgtcgat aaaactcgat
      121 ccactggccg tagcccaaga acttggtttc gacggggtag tagagaactc cattgacggc
      181 gtgagtgatc gcgatttcgt tgcagagttt ctatttgtca tcgccatgat tggcgtgcac
      241 ctttctcgcc taggtgaaga aatttgtttg tggtcatcac gcgaatttgc ctgggccaaa
      301 ttagatgatg cttggtcaac tggatcatcg attatgccgc agaaaaagaa tccagatgta
      361 gcagaacttg cacgtggtaa atctggtcgg ttaatcggtc acgtgacttc aatcttgact
      421 gtgctcaaag gtttgccatt cgcatacaac cgcgatttac aagaagacaa agagtcagtt
      481 atcgatgcga tcgaaactat gtctttaatt ttgccggcca tgactggcac agttgccact
      541 ttagaatttg ataccgatcg aatgttagcc agtgcacccg aaggttttgc gcttgctaca
      601 gacattgctg aatgggttgt aaaacaagga gtcccattcc gtgaggcaca cgaaattgcc
      661 ggagcctgtg tgcgaaaggc tgaacaatta ggcacggatc taaacggact ctcagatgat
      721 gacttccagt caatccatcg cagtctgacc tctgaagtgc gcagtgtgct tacagttcaa
      781 ggatcaatca attcacgcaa taccgtaaat ggcactgcac ctgacgcagt tcgccgtcag
      841 attgaagtag ccaaagataa agtcgctctg ctaactaagt ggagcactaa tcttccacat
      901 aaagtttaaa gattttcgta gactttacgg cgatgatcta tgtcgattac atcgacaact
      961 aactcactat ctgtaatcgt ataaataatt ctgaaatttc ccacacgtac tctgtattta
     1021 tctgagtttg ctaattttat tgaattacgg ggccgaggat ttatcgaaag agcggttaag
     1081 atttttcgaa cgctggcgtt gtcaaaagcc gataactttt gcaggttttt tagagccttc
     1141 ttgctgatca aaacctgata ggtcatgaaa ggccaagttc acgaaagacc tgttccattg
     1201 gaattccggg aacatccaat tcgccgcttt cttccagctc ttcgagcatt tttaaatctt
     1261 gaatttcctc ccaagcatcg acgagttttt catagacttc agcagaaatc atgaccgcca
     1321 ccactttgcc atgtttctcc aaaaagactt cttcaactgc agaccttgtt acagcgtcag
     1381 cgagattttc gcgcgcttca gaaatcgaat atgtagccat aaaaaaaatg tacaataatt
     1441 aacttgaatt gtacagacta tccacaggct aaatttcagt tatttcctaa gattccgcca
     1501 agatagttaa gtgactgaca tccttgctga actgggctgg cgaaacctag tctcacagca
     1561 cactcctgag ctacccgaag aattagctaa aggttcagtc acgctctact gcggttttga
     1621 tccgactgca ccaagtttgc acatgggtaa cctggcccaa atcatcacga tgtctaggtt
     1681 ccaaaaactt ggtcatacac cgatcgccct ggttggggga gccaccggat caatcggtga
     1741 cccatctggc aaaaatgccg aacgtacttt gaatacaact gacaccgtgg gtgaatgggt
     1801 tgagcgcctg cgcaaacaac tagaaccatt ttttgaattc agtggcaaga atgccgcaat
     1861 catgtccaac aactacatct ggacgcacaa catggatgtc ct
//
LOCUS       DBNHVN010000233         1547 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1603230, whole genome shotgun sequence.
ACCESSION   DBNHVN010000233 DBNHVN010000000
VERSION     DBNHVN010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1603230"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="AC16Y1_04400"
     CDS             <1..348
                     /locus_tag="AC16Y1_04400"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR5831735.1"
                     /translation="NWLAGHRGVDFRGQIGDLVFATGHGIVVYADRLAGKGVVVISHG
                     LVRTTYEPLVALVEVGDQVTPGQVIGSLTTGESHCATRETVWCLHWGAIRNGKYLNPL
                     LLVYPKVRLLPLT"
     gene            complement(361..1281)
                     /locus_tag="AC16Y1_04405"
     CDS             complement(361..1281)
                     /locus_tag="AC16Y1_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HIR5831736.1"
                     /translation="MSSLQVDFAGLLDRFIQYIELELGRSKNTATAYRKDLENLIEFV
                     TAQGCTQIADVGIMELRGWLAGQRNSGLTSATLARRATAARTFFAWATQQKQLTEDPA
                     ASLVIPKVSKRLPNVLAQDQATEVMNRAALRADDDSPEHIRDLAILELMYATGIRVGE
                     LVGLDISDIDQTRRTIRVTGKGSKQRVVPYGSIADRAISSYLKSARNMLANESSGLAL
                     FLGSRGKRIDQRVVRKMVTELLASLEGIPDLSPHGFRHSAATHLIEGGADIRAVQEML
                     GHASLSTTQLYTHVSMDRLRAVFEQAHPRA"
     gene            complement(1370..>1547)
                     /locus_tag="AC16Y1_04410"
     CDS             complement(1370..>1547)
                     /locus_tag="AC16Y1_04410"
                     /inference="COORDINATES: protein motif:HMM:NF014533.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR5831737.1"
                     /translation="VEAALRSGSLSTANWANALGRKVWGVPGPITSATSAGVHAGIRN
                     GTMQILLEVTELIT"
BASE COUNT          398 a          353 c          350 g          446 t
ORIGIN      
        1 aattggctag ctggacatcg tggggttgac tttcgtggac aaatcggcga tctagttttt
       61 gctaccggcc atggcatcgt ggtttatgcc gatcgactcg ctggtaaagg tgttgtcgta
      121 attagtcatg gtttagttcg aactacgtat gaaccattgg ttgcgctagt tgaagttggc
      181 gaccaagtga cacctggtca agtaatcggc tcccttacca ccggagaaag tcactgtgca
      241 actagagaaa ctgtttggtg tttacattgg ggagcaatca gaaacggaaa atacttaaat
      301 ccgttactgc tggtttatcc aaaagtgcga cttttgccat tgacctaata gttattcaaa
      361 ttatgcgcgc gggtgagcct gttcaaaaac tgcgcgtaat ctatccatcg agacgtgcgt
      421 atacagctgc gttgtcgaca gacttgcgtg tccaagcatt tcttgtactg ctctgatgtc
      481 tgccccacct tcaattaaat gagttgcggc cgagtggcga aaaccgtgtg ggcttaagtc
      541 aggaatgcct tcgaggcttg ctaatagctc agttaccatt ttgcgaacaa cccgttgatc
      601 tatgcgcttt cctcttgagc caagaaatag cgctaaccca cttgattcat tggccaacat
      661 atttctagca gactttaaat atgacgaaat ggctcggtca gcaattgatc cataaggtac
      721 aacgcgttgc ttagaccctt taccagtgac tcgaattgtt cgccgagttt gatcgatgtc
      781 tgagatgtct agacctacta gttctccaac acgaattccg gtggcataca ttaattccaa
      841 aattgctaga tcgcgaatat gttccggtga atcatcgtcg gcacgtaacg cagcacgatt
      901 cattacttca gttgcttgat cttgtgccag tacatttggc aatcgtttac taactttagg
      961 aataactaaa ctagctgctg gatcttcagt taactgtttt tgttgtgttg cccaggcaaa
     1021 aaaagttcta gctgccgttg cccgcctggc caatgttgcc gaagttagtc cgctattacg
     1081 ctgtcctgcg agccagccgc gcagttccat aattccaaca tctgcaattt gcgtacaacc
     1141 ctgtgcagtt acaaattcga ttaagttttc caaatccttg cggtaagcag ttgccgtatt
     1201 tttactccgg cctaattcga gttctatata ttgaatgaag cgatcgagaa gtccagcaaa
     1261 atcgacttgt aacgaactca tttatatacg gtgacaagta aaagcacttt tagcaagtac
     1321 atttagcagt tggctttaag tgtcagaggc ttttcactgt aaagcgaagt taagttatca
     1381 attcagtgac ttccagcaag atctgcatag tgccgtttct aattccagca tgaacgccag
     1441 ctgaagttgc actggtgatt ggaccgggca cgccccagac tttacggcca agtgcattcg
     1501 cccagtttgc cgtagataaa gagccagatc taagtgcagc ctcaaca
//
LOCUS       DBNHVN010000234         1486 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1381490, whole genome shotgun sequence.
ACCESSION   DBNHVN010000234 DBNHVN010000000
VERSION     DBNHVN010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1486)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1486)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1486
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1381490"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..177
                     /locus_tag="AC16Y1_04415"
     CDS             1..177
                     /locus_tag="AC16Y1_04415"
                     /inference="COORDINATES: protein motif:HMM:NF028226.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HIR5831738.1"
                     /translation="MHVLVPGAWTISQGHDLLEDLEEAIIAALPDTTVHTHVEPIEDP
                     RSWADIPAGSYIWD"
     gene            complement(174..485)
                     /locus_tag="AC16Y1_04420"
     CDS             complement(174..485)
                     /locus_tag="AC16Y1_04420"
                     /inference="COORDINATES: protein motif:HMM:NF024232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HIR5831739.1"
                     /translation="MSVKKAWEIYRALAILVGTVLATLTFIAMPYRYLIAKEEILAYS
                     LAWMIHGYLFPLYVIATFVLSLKLNWPIGKTILIMLAGTVPLMSFYTERKVAVEVKPR
                     I"
     gene            complement(482..1234)
                     /locus_tag="AC16Y1_04425"
     CDS             complement(482..1234)
                     /locus_tag="AC16Y1_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014193.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial respiratory chain
                     complex IV assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HIR5831740.1"
                     /translation="MARYFSSRLIGIHLFALAAVTTCLWLGNWQWQRAQTNVQVVTVN
                     GQTDFQTLSPLRNYLPPSSVGVPTTVTGTWQPDGRIVFENRPANGELLTIENQEQFAQ
                     VKKGVWVLDILNLETGSSVAVVHSWLPTADEGQELTGQVTVEGVLQPSELALTQTLVE
                     LPNYITTEVIVKKAISTVHDGYFVSAKPIIGNQKVSPIVPVAQETKLQWRNVIYTGNW
                     IIFAIIIGAMWWRIIQEEVEPSNKQLINGVAK"
BASE COUNT          407 a          334 c          320 g          425 t
ORIGIN      
        1 atgcatgttt tagttcctgg tgcttggact atttcccaag gccacgactt acttgaagat
       61 ttagaagaag caattattgc ggcgctgcca gatacgactg tgcacaccca cgtcgaacct
      121 attgaagatc cacgctcttg ggcggatatt ccggccggtt cttatatctg ggactaaatt
      181 cttggtttaa cttcgacagc tacttttcgt tcagtgtaga acgacatcaa cggaacagta
      241 ccggccagca taatcaaaat agttttacct atcggccaat ttaatttcaa cgacaaaaca
      301 aaagttgcaa ttacataaag tggaaataga tacccatgaa tcatccaggc aagtgagtaa
      361 gcaaggattt cttctttcgc aattaagtag cgatatggca tggcgataaa agtaagtgtg
      421 gccaacactg tgcccaccag aattgctaat gctcgataaa tttcccaagc ttttttaacg
      481 ctcactttgc tactccattt attagctgtt tatttgaggg ttcaacttcc tcttgaataa
      541 tacgccacca cattgcgccg atgatgatgg cgaaaataat ccagtttccg gtgtaaatca
      601 catttcgcca ctgcagttta gtttcctggg caactggaac tatcggacta actttctggt
      661 tgccgataat tggctttgca ctaacaaagt aaccatcgtg cacagtcgaa attgcttttt
      721 tgacgatgac ttctgtcgtg atgtagttag gtagctcaac cagcgtttgc gtgagcgcta
      781 actcacttgg ttgtaaaacg ccttccactg ttacctgacc ggtgagctct tgcccctcat
      841 cagccgttgg taaccagcta tgcacaactg caacacttga tcctgtttct aaattaagta
      901 tgtccagtac ccaaacacct tttttaactt gtgcaaattg ctcttgattt tcaatggtca
      961 gcagttcgcc atttgctggt cggttttcaa aaacgattcg cccatctggt tgccaagttc
     1021 cagtcactgt agttggcact ccgacagaac ttggtggtaa ataatttcta agcgggctaa
     1081 gtgtttgaaa atctgtctgc ccgttgacgg tgactacttg cacattagtc tgggcgcgtt
     1141 gccattgcca gttacctaac caaaggcaag tagttacagc agccagggca aataagtgaa
     1201 tcccgatcag gcggctagaa aaataacgag ccatcgaggt tattttgatt cagtatggcg
     1261 attgacgtcg atccgcttga tatgtctagt catgctgatc aacaaaagaa ttaatgcaat
     1321 taaaagcaag gtaaaaacaa cggcggctaa tggaccggga tgaacggttt cggcatttag
     1381 tattcggggc gctggagttg gcgaggcagc actcaacttg gtcaagatcg aaaacatcga
     1441 agtgccgtcc tcaattaaag aattaactgt ttctctatct taagcc
//
LOCUS       DBNHVN010000235         2907 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_268162, whole genome shotgun sequence.
ACCESSION   DBNHVN010000235 DBNHVN010000000
VERSION     DBNHVN010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_268162"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="AC16Y1_04430"
     CDS             complement(<1..706)
                     /locus_tag="AC16Y1_04430"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIR5831741.1"
                     /translation="MADVLTSIVEGVREDLADRKRATTLSELTDKISARSAALPAATN
                     LLSRPFSVIAEVKRSSPSKGDLAAITDPKALADKYASGGAQVISVLTERRRFGGTLAD
                     LVAVRDAVEIPILRKDFMVDEYQVYEARAFGADVILLIVAAIGDSQLQELDELAKQLG
                     LSVLVEVHNEDELERALELNPQLIGVNARNLKTLDVDLAVCERLLPLIPKNALAIAES
                     GISSLKEVERLVEAGAR"
     gene            complement(767..961)
                     /locus_tag="AC16Y1_04435"
     CDS             complement(767..961)
                     /locus_tag="AC16Y1_04435"
                     /inference="COORDINATES: protein motif:HMM:NF042744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HGxxPAAW family protein"
                     /protein_id="HIR5831742.1"
                     /translation="MSGNNHGQTVAAWTLSIIVMIAFLVGSIGVVLDNRPIFWTGVGL
                     IGVGLIAGKVLSAMGYGKKS"
     gene            complement(1000..1584)
                     /locus_tag="AC16Y1_04440"
     CDS             complement(1000..1584)
                     /locus_tag="AC16Y1_04440"
                     /inference="COORDINATES: protein motif:HMM:NF021079.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trp biosynthesis-associated membrane protein"
                     /protein_id="HIR5831743.1"
                     /translation="MRGLYRSLLLTLAICSLIIFVVSGQTWATVNFMEANSPNLVYEF
                     SGRQLQPIIAGVIVVPIVGVVGIIAAKKLLQRIIGFFIFLSGLTVAYYSFQVGRNWVE
                     LLDPLVTNKVGLTGIEYVYETTQANNVLILPAIAIAAVGFMFTVRNFDQAKKTAAYDA
                     PVAGISTLTPWQALDSGLDPTISVSDGASGITPS"
     gene            complement(1585..>2907)
                     /locus_tag="AC16Y1_04445"
     CDS             complement(1585..>2907)
                     /locus_tag="AC16Y1_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018835581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HIR5831744.1"
                     /translation="QTHWSGNVPVGLPTSGDPIETLRATLEILHTPDVNIGVSANELP
                     PLTGGVVGYLAYDIVRRFEKLPNLTVDSLQLPELAMLIATDLAVLDHVDGSVTLIANA
                     INYDNSPERVELAWADAVSRLDNMEDNLARPLASSVSSYDQNAKPVITNETLSSDYQA
                     AVEVAKDHIRAGDAFQIVLSQRFSTPCTASALDVYRILRTTNPSPYMFLFRIPKVTSA
                     ESELSNEVAFDLVGSSPEALVKLTNGQAMLHPIAGTRPRGEDQIADDKLAEELLADTK
                     ERAEHLMLVDLGRNDLGRVCLPGTVDVSDFMSIEKYSHVMHIVSTVVGQIDPDVNGVD
                     LIAATFPAGTLSGAPKPMAMKIIEQLEPTKRGFYGGCVGYFDFAGNIDTAIAIRSAVI
                     KNQTAYVQAGAGIVADSIPTLEDAECNAKAAAVLRAVAVANTLPTGDR"
BASE COUNT          808 a          698 c          610 g          791 t
ORIGIN      
        1 ctctggcacc cgcttctacc aagcgttcga cctccttgag cgaacttatg cctgattctg
       61 caatggctag agcatttttt ggaatgagtg gaagtagtcg ctcacatact gctaaatcaa
      121 catctaaagt cttgaggttt cgcgcgttta ccccgattag ttgtggattc agttccagtg
      181 cccgttcgag ttcatcctca ttgtgaactt caactaatac agataagcca agttgttttg
      241 ctaattcatc taattcctgc agctgcgaat ccccaattgc cgccacaatt aacaaaatta
      301 cgtcagcgcc aaatgctctg gcttcataaa cctgatattc atcgaccata aagtctttac
      361 gcaatatggg aatttcaacg gcgtcgcgca cggcaactaa atcagctagc gtgccaccaa
      421 atctgcgccg ttcagtaagc acactgatta cttgggcgcc ccccgatgca tacttatccg
      481 caagtgcttt tgggtcggtt attgcggcta agtcaccttt actgggacta cttcgcttta
      541 cttcagcaat cacgctaaac ggtcgggaaa gtaaattagt tgctgccggt agcgctgccg
      601 atcgagctga aattttgtca gttagttccg aaagtgttgt cgcgcgcttt cgatcggcta
      661 aatcttcgcg aactccttcg acaatggatg ttaaaacatc agccatgtcg agctcacttt
      721 cgcattaatg aaccttaagt ttattggaaa gtaagtgatg agttatttaa gattttttgc
      781 cgtaacccat ggcagaaagc actttacctg caattaagcc aacgccgatt aagccaacgc
      841 cagtccaaaa aattggtcga ttgtctaata caacaccgat tgagccaacc aagaatgcga
      901 tcatcacaat tatggatagt gtccaagcag ctacggtctg accgtggttg ttccctgaca
      961 ttttgctcct accaattatt ggcgaatact caccctagtt taactaggtg taattccgga
     1021 agcgccatca gagacactta tcgtcgggtc aagtccagaa tctaaggctt gccacggtgt
     1081 cagcgtgctg atgccagcaa ctggtgcatc gtaagccgcc gttttctttg cttgatcaaa
     1141 atttctaacc gtaaacatga atccaactgc agctattgca attgctggaa gaattaatac
     1201 attatttgcc tgagtagttt catatacata ctcaatccca gttaaaccaa ctttattggt
     1261 gaccaatgga tcgagtagtt caacccagtt acggccaact tgaaaactgt aataggcaac
     1321 ggttaagcca gatagaaaaa taaaaaaccc aataattctt tgcaatagtt tcttcgcggc
     1381 aataattcca acaacaccaa caatcggcac aactattacc ccagcaataa tcggttggag
     1441 ttggcgacca ctgaattcat aaaccaagtt cggactattg gcttccataa aatttacagt
     1501 ggcccaagtc tggccactga ctacaaaaat aattaacgaa caaatggcta acgttaaaag
     1561 cagacttcga tacagcccgc gcatttaacg gtcaccagtt ggcaaagtat tagccacagc
     1621 cactgctcga agtaccgcag cggctttcgc attacattca gcatcttcca gagtcggtat
     1681 tgaatcagcc acgatacctg caccggcctg tacatatgca gtttgattct tgataactgc
     1741 acttcgaatt gcaattgcag tatcaatgtt gccggcaaag tcgaaatacc caacacagcc
     1801 gccataaaaa ccgcgcttgg ttggctctaa ctgttcaata attttcattg ccattggttt
     1861 tggcgcgccc gataaggtgc cagctggaaa cgtggccgca attaaatcca cgccattaac
     1921 gtctggatca atctggccga ccacagttga aactatgtgc ataacgtgac tgtatttttc
     1981 aatcgacata aaatcgctca cgtcaacggt gcccggtaag caaactcggc ctaagtcatt
     2041 tcgacctaaa tcaaccagca ttaaatgttc agctcgctct ttagtatcgg ccaataattc
     2101 ttcggcaagt ttgtcgtcag caatttgatc ttcaccacgc ggtctagtac ctgcaattgg
     2161 gtgcagcatt gcttggccat ttgttaattt cacaagtgct tcagggcttg aacctactaa
     2221 gtcaaatgcc acttcgttac taagttcact ttcagcgcta gtaaccttcg gaattctaaa
     2281 taagaacatg tacgggcttg gatttgtggt gcgcaatatt cgataaacat ctaaagctga
     2341 tgcagtacaa ggagttgaaa atcgttgtga gagcacgatt tgaaatgcat caccggcacg
     2401 aatgtgatct ttggcgactt caactgcggc ttggtaatcg ctagagagtg tttcatttgt
     2461 gataactggc ttggcatttt ggtcataaga tgacaccgaa gatgcaagcg gcctggccaa
     2521 gttgtcttcc atgttgtcta agcgactgac tgcatccgcc caagctaact cgacccgctc
     2581 gggtgagttg tcatagttaa tcgcatttgc aatcaaggtg acggacccgt caacatgatc
     2641 tagaacggct aagtcggtag cgatgagcat agctagctca ggcagttgca aactatcaac
     2701 ggttaaattt ggtagctttt caaaacggcg cacgatgtcg taagccaaat aaccaacaac
     2761 tccgccagtt aacggaggta attcatttgc actaacacca atgtttacat caggtgtgtg
     2821 cagtatttct aaggtagcgc gaagtgtttc gattggatca ccactagttg gtaagccaac
     2881 tggtacattg cccgaccaat gtgtttg
//
LOCUS       DBNHVN010000236         4843 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_935342, whole genome shotgun sequence.
ACCESSION   DBNHVN010000236 DBNHVN010000000
VERSION     DBNHVN010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_935342"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..551)
                     /gene="ruvC"
                     /locus_tag="AC16Y1_04450"
     CDS             complement(30..551)
                     /gene="ruvC"
                     /locus_tag="AC16Y1_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009611473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR5831745.1"
                     /translation="MRVLGIDPGLTRCGVGVIDIDNRAKPAFVHVTVIKTSSDLDLSA
                     RLGLLHSELKIVVANFAPDVMAVERVFSQLNVKTAMGTAQASAVAMLIANDLNLPVHL
                     YTPTEVKAAVTGSGRADKKQITQMVTRHLKLTELPKPADAADALALALCHAWRGLSQE
                     RIATAAKKIGVTI"
     gene            complement(625..1377)
                     /locus_tag="AC16Y1_04455"
     CDS             complement(625..1377)
                     /locus_tag="AC16Y1_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010988230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR5831746.1"
                     /translation="MSGHSKWATTKHKKAVIDARRGKLFAKLIKNIEVAARTGGGDPA
                     ANPTLFDAIQKAKKSSVPNDNIDRAVKRGSGLEAGGAEYQTIIYEGYGPNGVALLVEC
                     LTDNRNRAASEVRVAMTRNGGNMADPGSVSYLFNRKGLILVSSNSTTEDQLLEAVLDA
                     GAQEVNNLGETYEVICEATDLVKVRQAVQAAGIDYESAEATFLPDLTIPLDTEAAGKM
                     FKLIEALEDSDEVQNVYANFDVSDEVLAKLDA"
     gene            complement(1374..1964)
                     /gene="pdxT"
                     /locus_tag="AC16Y1_04460"
     CDS             complement(1374..1964)
                     /gene="pdxT"
                     /locus_tag="AC16Y1_04460"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014134593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HIR5831747.1"
                     /translation="MSTPNIGVLAIQGDVREHVNHLTACGVQTTLVKTVESLAIVNAL
                     VIPGGESTTMSILTVKNNLIEPLRAASKNIPMYGSCAGLIMLADRITDGRADQETIGG
                     LDITAQRNAFGRQVDSFEIDLEIAQIGEPSFHAVFIRAPLVSQVGASVEVLATLPNSD
                     QVVAVRQDNLMATSFHPEIVPDFRIHKYFVEMVKAA"
     gene            complement(1967..2863)
                     /gene="pdxS"
                     /locus_tag="AC16Y1_04465"
     CDS             complement(1967..2863)
                     /gene="pdxS"
                     /locus_tag="AC16Y1_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010118742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HIR5831748.1"
                     /translation="MASEAGVTGTTKVKRGMADMLKGGVIMDVVDAQQAKIAEEAGAV
                     AVMALERVPADIRAQGGVARMSDPDMIEGIINAVSIPVMAKARIGHFVEAQVIQSLGV
                     DYIDESEVLTPADYAHHIDKWKFTVPFVCGATNLGEALRRITEGAAMIRSKGEAGTGD
                     VSNAVTHMRTIRAEINRLKSMSPDELYVAAKELQAPYELVVEVAQKGKLPVVLFTAGG
                     IATPSDAAMMMQLGADGVFVGSGIFKSGNPVQRAKAIVQATLHYEDPAKVADASRGLG
                     EAMVGINVADIPVPHRLAERGW"
     gene            complement(2930..3472)
                     /locus_tag="AC16Y1_04470"
     CDS             complement(2930..3472)
                     /locus_tag="AC16Y1_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831749.1"
                     /translation="MNAEIIWLVAFALFAIWYLSHQAGRLDRLHHRIEVAMVGLDGHL
                     NRRAGIAAELVSLNFLDPASEAYLMQSAHDVLAGADLPDPSRLSDESELTEALADAFE
                     DEEDVLEFRKDAAISLLLDELLTVCTKISLARGFHADAVRDCLNLRDQIFVKMFRLAG
                     WAKRPVAIAFDDRIPSGLGR"
     gene            complement(3462..4658)
                     /locus_tag="AC16Y1_04475"
     CDS             complement(3462..4658)
                     /locus_tag="AC16Y1_04475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014146000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5831750.1"
                     /translation="MKIGIVCPYAWDVPGGVSAHINDLAIALQKIGHQVSVLAPAETE
                     DHLPDFVTSVGQPRAVKYNGSVARLSFGPVTARKVSKWIEDGQFDVLHVHEPLAPSVS
                     VLAVWAAKGPIVATWHSSVDKSRMILSLAKLAQTAMEKISARIAVSEAARTTLINHVG
                     GDAVIIPNGVDTSAFIDAHPISGWPGEGKALVFLGRVDEPRKGLDILLAAFPIVRKEF
                     PNLRLLIAGPGDPEELIDDVPIADRNAIVHIGKISNDEKPDVFASGNIYVAPNTGGES
                     FGIVLLEAMASGVPVVASNLHAFERVLEYGKSGVTFKNENSQDLAKVLIDLLSDEQKL
                     AQLKAAGYARSAIFDWTLVASRVLEVYESVHLPGVKVEPDFAGQMLGRLSKLRIKPER
                     TDIYER"
     gene            complement(4655..>4843)
                     /locus_tag="AC16Y1_04480"
     CDS             complement(4655..>4843)
                     /locus_tag="AC16Y1_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831751.1"
                     /translation="FDDEIHYPKEKHSGRELIKAAQDLTAQVATRFERHIRAHPEAWH
                     QLQPIWSDLRAESSVDGK"
BASE COUNT         1281 a         1192 c          976 g         1394 t
ORIGIN      
        1 ctctaaaact ttgccactaa taatcgcaat caaattgtca ctccaatttt ttttgctgct
       61 gtggcgatgc gttcctgact tagaccacgc caagcatggc aaagtgccaa cgccagagca
      121 tccgcggcat cagctggctt gggcaactca gttaatttca agtgtcgcgt aaccatttgg
      181 gtaatttgtt ttttgtcagc tcggccagaa ccagttacag cagctttaac ttcggttggg
      241 gtataaagat ggaccgggag atttaaatca ttagcgatta gcattgccac cgcgcttgcc
      301 tgtgctgtac ccatagctgt tttgacattt agctgactaa aaactcgttc aactgccatt
      361 acatctggtg caaaattcgc aaccacaatt ttaagttcac tatgtaaaag gcctaaccga
      421 gccgataaat ctaaatcgct cgaagttttt attacagtca catgaacaaa agctggtttg
      481 gctcgattat caatgtcgat gactccgaca ccacatcggg ttaatcctgg gtcaattcct
      541 agaaccctca ttcatgtcgc ctttccgaac atttgttcga aagcctatca ggatagaccg
      601 gcattcctag gtattgagaa agacttaggc gtccaatttt gccaatactt catcagacac
      661 atcgaagttt gcgtacacat tttggacttc atctgaatct tcaagggctt caatcaactt
      721 aaacattttt ccggcagctt cggtatccaa tggaatggtc aaatctggca agaaggtggc
      781 ttcagcagat tcataatcaa ttccggctgc ctgcactgcc tggcgaactt taactaagtc
      841 agtagcttcg caaattactt cgtatgtttc acctaagtta ttcacttctt gggcgccagc
      901 atctagtacc gcttcaagta attgatcttc cgtggttgag ttactcgaaa ctaaaattaa
      961 gccttttcga ttaaataaat atgaaactga acctggatca gccatatttc caccatttcg
     1021 ggtcattgcg acgcgaactt ctgacgcagc tcgatttcgg ttatctgtta aacactcaac
     1081 taaaagggct accccgtttg gaccgtatcc ttcgtaaata attgtttgat actcggcacc
     1141 gccggcttca agtcctgaac ctcgtttaac tgctcgatca atgttgtcat tgggtactga
     1201 gctctttttt gccttttgga tcgcatcaaa aagagttgga ttagctgcgg gatcaccacc
     1261 gccagtgcga gcagcaactt cgatgttttt tattaatttg gcaaagagtt ttccacgtct
     1321 tgcatctata acggcctttt tatgtttagt agttgcccat ttggaatgac cgctcatgct
     1381 gctttcacca tttctacaaa atatttgtga attctaaagt ctgggactat ctctgggtgg
     1441 aatgaagttg ccattaaatt atcttggcgt acggctacca cttgatcact attaggcaga
     1501 gttgccagca cttcgacact tgcgcctact tgagaaacca gtggagcccg aatgaatacc
     1561 gcatgaaaac ttggttcacc aatttgagct atttctaggt caatttcaaa tgaatcgact
     1621 tgtcgaccaa atgcattccg ctgggccgta atgtctaatc caccgatagt ttcttgatcg
     1681 gctcggccat cggtaattcg atcagccaac ataatgagtc cggcacaact tccatacata
     1741 ggtatgttct tactcgctgc tctaagcggt tcaatcaaat tattttttac cgtaagtatg
     1801 ctcatcgtgg ttgattctcc accggggatc acgagtgcgt ttactattgc cagactttca
     1861 actgttttaa ccaaagttgt ttgaacgccg caagcagtta aatgatttac gtgttcgcgc
     1921 acatcgcctt gtatggcgag tacccctatg ttcggggtag acatacttac cagccgcgct
     1981 ctgccaaccg gtgcggaact ggaatgtctg caacgttaat gccaaccatt gcttcaccta
     2041 agccgcgtga cgcatcggca acttttgctg ggtcctcata atgcagagtg gcctgcacaa
     2101 tcgctttagc tcgctgaacc ggatttccgg acttaaaaat accggaacca acgaaaacac
     2161 catcggctcc aagttgcatc atcattgctg catcagatgg cgttgctatg ccgccagcgg
     2221 taaatagcac aactggcaat ttaccttttt gtgcaacttc gacaaccaat tcgtaaggtg
     2281 cttgcagttc tttagccgca acataaagtt cgtctggact catcgattta agtcggttga
     2341 tttcagctcg gattgtgcgc atgtgtgtga ctgcatttga aacatcaccg gttccagctt
     2401 cacctttaga tcgaatcatg gccgcacctt ctgtgattcg tcgaagtgcc tcacctagat
     2461 ttgtcgcacc gcaaacgaag ggtacagtga atttccactt atcaatgtgg tgggcataat
     2521 ctgccggtgt taaaacttca gactcgtcta tatagtcaac acctaaactt tgaattactt
     2581 gcgcttcaac aaaatgtccg atgcgagcct tggccataac cggaatcgaa acggcattga
     2641 taattccttc gatcatgtct ggatcgctca tacgagcaac tccaccttgg gctcgaatgt
     2701 ctgctggaac acgctctaaa gccataactg caacagcgcc ggcttcttca gctatctttg
     2761 cttgctgcgc gtcaaccaca tccatgatga cgccaccttt aagcatgtct gccatgccac
     2821 gctttacttt ggtggtaccg gttacgcctg cttcgctggc cattaaatcc tctttttaaa
     2881 aactagattg ttccagataa aaatgatagc cgagtttcaa agtattggct taacggccta
     2941 aacccgatgg gattcggtca tcaaatgcaa ttgctacagg tcgtttagcc caaccggcaa
     3001 gcctaaacat tttcacaaaa atctgatccc gcagattcag gcagtcgcgg acggcatcag
     3061 catgaaatcc tcgagccaac gaaatcttgg tacatacagt taataactca tcgagcagca
     3121 aactaatagc cgcatctttt cgaaactcaa gcacgtcttc ttcgtcttcg aatgcgtcag
     3181 ccagggcttc agttaattca gattcatcac tcaaacgtga tggatccggt agatcggctc
     3241 cggctaatac atcgtgtgct gactgcatta aatatgcctc agaggctgga tctagaaaat
     3301 taagtgaaac tagttcagcc gcaattcctg ctcttcggtt caaatgtccg tcgagtccaa
     3361 ccatcgcaac ttcaattcga tgatgcagtc gatctagtct ccctgcttga tgtgaaaggt
     3421 accaaatcgc aaatagagca aaggctacta accagataat ttcagcgttc ataaatatca
     3481 gttctttcag gtttaattcg caatttactc aaccgcccca gcatttggcc tgcaaagtca
     3541 ggctcaactt ttactccagg aagatgaacg gattcataaa cttctaatac tcgactagca
     3601 actaatgtcc aatcaaaaat tgcactcctg gcatagccag cagctttcaa ctgcgctaat
     3661 ttttgttcat cacttaaaag atcaatcagt actttggcta aatcttgaga gttttcattt
     3721 ttaaaagtga ctccagactt tccgtattca agtacccgtt cgaatgcgtg taagttgctt
     3781 gctactactg gtacgccact tgccatcgct tcaagaagca cgatcccgaa agattcacca
     3841 ccagtgttag gggctacata aatgtttcca gatgcgaaaa catctggttt ttcatcgttt
     3901 gagatttttc cgatgtgcac aattgcattc cgatcagcaa ttggtacatc atcaattaat
     3961 tcttctgggt caccggggcc cgcaattaat aaacgtaaat taggaaactc ttttctgaca
     4021 attggaaacg cagctaaaag aatgtcaagg cctttacgtg gttcatcaac tctgccaaga
     4081 aatactaaag ctttaccctc gcctggccat cccgaaatgg ggtgtgcgtc tataaaagcc
     4141 gaagtatcta ctccgtttgg aataattact gcgtcgccac cgacatgatt tattaatgta
     4201 gttctcgctg cttcactaac agctatccgc gcagaaattt tctccattgc agtttgagcc
     4261 aatttagcca aactaagaat catccgagac ttatccacac tggaatgcca agtagcgacg
     4321 attgggcctt tagccgccca aactgctaaa acagaaacgc tcggtgccaa cggttcgtgc
     4381 acatgtagca catcaaattg tccatcttcg atccacttag aaactttgcg cgccgtaact
     4441 gggccaaaac ttaatctcgc cacagaaccg ttgtacttaa ctgcacgagg ttgcccaact
     4501 gatgttacaa aatctggtaa gtgatcttct gtttctgcag gtgctaaaac acttacttga
     4561 tgacctatct tttggagggc aattgctaag tcatttatgt gtgcagatac gccaccaggg
     4621 acatcccagg cgtacggaca aacgatgcca attttcattt accgtcaacg ctactttcag
     4681 cccttaaatc tgaccatata ggttgaagtt gatgccaggc ttctggatga gctcttatgt
     4741 gacgttcgaa acgcgttgct acctgcgccg ttaaatcctg tgccgctttt atcaattcgc
     4801 gtcccgagtg tttttctttc ggataatgaa tttcatcgtc aaa
//
LOCUS       DBNHVN010000237         1142 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_117617, whole genome shotgun sequence.
ACCESSION   DBNHVN010000237 DBNHVN010000000
VERSION     DBNHVN010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_117617"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="AC16Y1_04485"
     CDS             <1..629
                     /locus_tag="AC16Y1_04485"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003975738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase family protein"
                     /protein_id="HIR5831752.1"
                     /translation="FCEGKGPFRWAALSGDPKDIARTDQAVLELFPENEHLHRWIKMA
                     QERVAFQGLPARICWLGYGERDKAGVAFNDLVAKGEVSAPIVIGRDHLDCGSVASPYR
                     ESEAMLDGSDAIADWPLLNAMINISSGASWVSIHHGGGVGIGRSIHAGQVSVADGTPL
                     AAQKLARVLTNDPGMGVIRHVDAGYDLAVDVAKERHVHVPMLENGDYL"
     gene            632..>1142
                     /locus_tag="AC16Y1_04490"
     CDS             632..>1142
                     /locus_tag="AC16Y1_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017589777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5831753.1"
                     /translation="MPTNKTEDFSLVNVGLLVTNDSQLGNGPLGIVENAAVSVVDGKI
                     AWIGNSNLADKSLPTVNANSQCVIPGFVDSHSHIIFAGDRSAEFAARMAGVPYSAGGI
                     KVTTAATRSASSAQLKDNALRLIAEMHESGTTTIEIKSGYGLDVETEERSVEVAAELT
                     SEVTYLGAHV"
BASE COUNT          308 a          239 c          292 g          303 t
ORIGIN      
        1 tattttgcga aggtaaagga ccattccgct gggctgcgct atcaggtgat ccaaaagaca
       61 tcgctcgcac agatcaggca gtgcttgaac tttttccaga gaacgaacat ttacaccgtt
      121 ggataaaaat ggctcaagaa cgggtggcat tccaaggttt acccgcacga atttgctggc
      181 ttggatatgg tgagcgcgat aaagcgggtg tcgcatttaa tgacttagtc gcaaaaggcg
      241 aggttagtgc accgattgtt attggccgcg atcacttaga ttgcggttcg gtcgcgtcgc
      301 cttatcgtga atcagaagcc atgctggatg gatcagatgc gattgcggac tggccattac
      361 ttaatgcaat gatcaacatt tcatccggag cctcttgggt ttcaatacat catggcggtg
      421 gcgtgggaat tggtcgatca atccatgctg gtcaagtttc agttgcagat ggcacaccac
      481 tagcggcaca aaaacttgca cgcgtcctaa ctaatgatcc aggtatgggc gtaattcgac
      541 atgttgatgc tggctacgac ttagctgttg atgttgccaa ggaacgccac gtgcatgtgc
      601 cgatgctaga aaatggcgac tacctttaaa aatgccaact aataaaactg aggactttag
      661 tctggtcaac gttggactat tagtaactaa tgattcgcaa ctaggtaatg gccctcttgg
      721 catagttgaa aatgctgccg tcagtgtggt cgatggaaaa attgcctgga ttggtaattc
      781 aaatttagct gacaaaagtc tgccgacagt aaatgccaat agtcagtgtg taatccctgg
      841 atttgttgat agtcactcac acattatttt tgccggcgat cgatctgctg aatttgccgc
      901 caggatggct ggagttcctt attcagccgg tggaattaaa gtaaccacag cagcgactag
      961 gtcagcttca agtgctcagt tgaaagacaa tgcacttcgc cttattgctg aaatgcatga
     1021 gtctggcaca acaacaattg aaatcaaatc tgggtacggg cttgatgtag aaactgaaga
     1081 gcgtagtgtc gaagttgcag ccgaattaac ttcagaagtc acttatttag gcgcacatgt
     1141 gg
//
LOCUS       DBNHVN010000238         1159 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_268630, whole genome shotgun sequence.
ACCESSION   DBNHVN010000238 DBNHVN010000000
VERSION     DBNHVN010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_268630"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="AC16Y1_04495"
     CDS             <1..744
                     /locus_tag="AC16Y1_04495"
                     /inference="COORDINATES: protein motif:HMM:NF013183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HIR5831754.1"
                     /translation="EVIFTAGTLINPAAAGLIASSSIDEVPVKKIPYVDILVTGDELI
                     DGGVAAPGQIRDSLSIQIPGWITALGAQVGQLTRVADDEGQTRNALASCLAPIVITTG
                     GTAHGDYDYVRRALLDLNAELLIDEINMRPGHPSVLAKLPSGQLVASLPGNPLAAVVS
                     FLTLVAPAINRLLGQQLNKLGSATLAQEFPTDQTRIVPVQIENGRAVQSKFRGSAMLR
                     GLAQADALAVISSGNNPLGTTVRLLNLPW"
     gene            complement(748..939)
                     /locus_tag="AC16Y1_04500"
     CDS             complement(748..939)
                     /locus_tag="AC16Y1_04500"
                     /inference="COORDINATES: protein motif:HMM:NF022832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HIR5831755.1"
                     /translation="MINQIDQNIAFAGPKPEVIATHLKKFWTPQMRSDIYKEVQAKPD
                     EFSADVRKALDKLHSEAKA"
     gene            complement(965..>1159)
                     /locus_tag="AC16Y1_04505"
     CDS             complement(965..>1159)
                     /locus_tag="AC16Y1_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006380534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831756.1"
                     /translation="SDRVPAGVVYTTFHHPMSGANVITTEFSDWATNCPEYKVTAVEI
                     RPADSSADSVNSLDDVLMKA"
BASE COUNT          292 a          254 c          265 g          348 t
ORIGIN      
        1 gaagtaattt ttactgctgg caccttgatc aaccctgctg cagctggatt gattgctagt
       61 tcgagtattg atgaagttcc ggttaaaaaa attccttacg tagatatttt ggttactggc
      121 gacgaactta ttgatggtgg tgttgcggct ccaggccaga ttcgtgattc actatcgatt
      181 caaattcctg gttggattac tgcactgggt gcccaagttg gtcaactaac ccgagttgca
      241 gatgatgaag gccaaacaag aaatgcttta gcaagttgcc tggcgccaat tgtcattact
      301 acgggcggaa ctgcgcacgg tgactatgac tatgttcgcc gcgcgttact tgatttaaat
      361 gccgagttac tgatcgatga aataaacatg cgccctgggc atccaagtgt gctagccaaa
      421 cttccaagtg ggcaattagt agcaagtttg cctggtaacc cacttgcggc ggtagttagt
      481 tttctaactc ttgtggcacc agcaataaac cgacttctcg ggcaacaatt gaacaaactt
      541 ggttctgcca ctctggctca agagtttcct actgatcaaa cccgaatagt tccagttcag
      601 attgaaaatg gtcgagcagt acaaagtaaa ttccgtggct cggcaatgtt acgtgggctt
      661 gctcaagcag atgcgctagc tgttatttca tcaggcaata acccgctggg tactactgtt
      721 cgccttttga atttaccttg gtaaaaatta cgctttggct tcgctatgaa gtttgtctag
      781 agcctttcga acatcagcac taaattcatc tggcttagcc tgaacctctt tatagatatc
      841 tgatcgcatt tgaggcgtcc aaaatttctt aagatgcgta gcgataactt ctggttttgg
      901 tccagcgaat gcaatgtttt gatcgatctg attgatcatt gccttcagtt tgttaacgtc
      961 cactttaggc tttcattaaa acgtcatcta atgagttgac gctgtccgca cttgaatctg
     1021 cagggcgaat ttcaactgct gtaactttgt actcgggaca gttggttgcc caatcagaaa
     1081 attctgtcgt aattacattt gcaccgctca ttggatgatg gaaagttgtg taaaccacac
     1141 ctgctggcac gcgatcaga
//
LOCUS       DBNHVN010000239         3717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_46578, whole genome shotgun sequence.
ACCESSION   DBNHVN010000239 DBNHVN010000000
VERSION     DBNHVN010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_46578"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /gene="guaB"
                     /locus_tag="AC16Y1_04510"
     CDS             <1..1158
                     /gene="guaB"
                     /locus_tag="AC16Y1_04510"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013735868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR5831757.1"
                     /translation="EVDRLCGRYRISGVPVVDQEGVLVGIVTNRDMRFEDDKSRLVRD
                     VMTQMPLITGRPGMSGDDALSLLRINKIEKLPLVDDSGKLTGLITVKDYVKSDRYPLA
                     TKDSAGRLMVGAAIGVGEDAFQRGLTLVAAGADVIVVDTAHGHQRAVLEMVTRMKSAS
                     SAQVIGGNVATREGAQALIDAGADAVKVGVGPGSICTTRVVAGVGVPQITAIYEASLA
                     AGPAGVPVIGDGGLQYSGDIAKALVSGADTVMLGSLLAGCEEAPGQLVFVNGKQYKQY
                     RGMGSLGAMQSRGENRSFSKDRYFQDDVLSEDKLVPEGIEGQVPYRGQLAAVAHQLVG
                     GLRASMGYAGAGTIAELKQNGKLIRITPAGLKESHPHDVQLTVEAPNYHGR"
     gene            1167..2279
                     /locus_tag="AC16Y1_04515"
     CDS             1167..2279
                     /locus_tag="AC16Y1_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006604932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HIR5831758.1"
                     /translation="MSDVEVGRAKRGRIAYSFDDVSLVPSRRTRDPEVVSLNWKIDAY
                     NFSHPLIAAPMDSVVSPTSAKTFSELGGLAVLNLEGIWTRYQEPEKELAKISKLEPGE
                     AIKKLQQLYAQPIQPDLITERIGQMRQAGLTVAASLSPQRTAQYASQVVSAGVDIFVI
                     RGTTVSAEHVSAGSEPLNLKEFIYKLDVPVIVGGCATYTAALHLMRTGAAGVLVGFGG
                     GAAHTTQDVLGVRVPMASAVADVAAARRDYLDESGGRYVSVIADGSMGHSGDVAKAIA
                     LGADAAMVGSIFARATDAPGQGKHWGLEATHPDLPRGELVELGTSGTLTEIVNGPSNA
                     TDGTMNLVGALRRSLATCGYSDLKEFQRCEVVVTPR"
     gene            2362..>3717
                     /locus_tag="AC16Y1_04520"
     CDS             2362..>3717
                     /locus_tag="AC16Y1_04520"
                     /EC_number="1.2.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF006916.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinic semialdehyde dehydrogenase"
                     /protein_id="HIR5831759.1"
                     /translation="MDNVVATSGDSVTPISPFPGQTIQTVPLSSIDDVKVAVAQARAA
                     SETWAKTSIEDRIKIFDRYYELLFENQELLQDVIQKETGKARSHAAAEIFGVAMIAAH
                     YAKISKKALSPERRVGIFPVLTKTKVLAQPKGVVGIISPWNYPLTLSIYDAIPAMLAG
                     NTVVIRPDFQTAWSAIHAVDLLTQAGLPKGVATLVIGDGPTVGAALIDEVDYICFTGS
                     TATGRKVGAQAGARLIGASLELGGKNPMIVCKDANLNTFLESAVQGCFTSAGQLCISI
                     ERMFIHEDIYEEFLTALVARVKKIQLGASLTWDYEVGSLGSEAVLNRVEKAVATALSE
                     GAVAVVGGKSRPDIGPFVYEPTVLTGVTKDMKIYHEEVFGPVVYVLPFKDNEEAIRMA
                     NDSEYGLSASVISRDLDQAAQIASRIRSGSVNVNDGFAAAFGSVAAPMGGMGQSGLGR
                     RH"
BASE COUNT         1025 a          755 c          939 g          998 t
ORIGIN      
        1 gaagttgatc gactttgtgg gcggtaccga attagtggcg ttccagtagt agatcaagaa
       61 ggcgttttag ttggcattgt gacaaatcgt gacatgcgat ttgaagatga caagagccga
      121 ttagtccgtg acgtcatgac gcagatgcca cttatcactg ggcgaccagg tatgagtggt
      181 gacgatgcac tctcattgct ccgaataaac aaaattgaaa aactacctct tgtcgatgat
      241 tctggaaaat taactggact aataacggtc aaagattatg taaaaagtga tcgttatcca
      301 ctggcaacaa aagattcagc gggacgacta atggttggcg ctgcaattgg tgttggtgaa
      361 gatgcattcc aacgaggcct aactcttgtt gcggcaggag ccgatgtcat cgtcgtagat
      421 acagcacatg gacatcagag agccgtactc gaaatggtca ctcgaatgaa gtcagcatca
      481 agtgctcaag ttattggtgg aaatgttgca acacgtgaag gtgctcaagc gttaattgac
      541 gctggcgctg atgcagtcaa ggttggagtt ggcccaggtt caatttgcac aactcgagtt
      601 gtcgctggtg tgggcgttcc acagatcacc gcaatttatg aagcatcctt agctgcggga
      661 cctgctggcg ttccagtaat tggcgatggt ggcttgcaat attcaggtga cattgcaaaa
      721 gcattggtat ctggcgcaga cactgtcatg ctcggctcac tcctagctgg gtgtgaagag
      781 gctcctggtc aattagtttt cgtgaatggt aagcaataca agcaatatcg cggaatggga
      841 tccctcggcg cgatgcaatc gcgaggcgaa aatcggtcat tttcaaaaga taggtatttc
      901 caagacgatg ttttatctga agacaaatta gttcctgagg gtattgaagg tcaggttcct
      961 taccgaggcc agctagccgc agtcgcacat caattagttg gtggacttcg cgcatccatg
     1021 ggctatgccg gtgctggaac tattgctgaa ctaaaacaaa atggaaaact aattcgaatt
     1081 actccggctg gcttaaaaga aagccatccg cacgatgttc aactaacagt tgaagcacct
     1141 aattaccacg gccgatagga gtcaagatgt cagacgtgga agtaggtaga gccaagcgcg
     1201 gacgcattgc atattcattt gatgatgttt cgttagttcc gagcaggcga actcgtgatc
     1261 cagaagttgt ttctctcaat tggaaaattg atgcctacaa cttttctcat ccgttaattg
     1321 ctgcgccaat ggattcagtt gtctcgccaa catctgctaa aacttttagc gaattaggtg
     1381 gactagcagt tcttaattta gaaggtattt ggacgcgcta tcaagaaccg gaaaaagaat
     1441 tagctaaaat ttcaaaactt gaaccgggtg aagcaattaa aaaattacaa caactttatg
     1501 cgcaaccaat tcaaccagat ctaattaccg agcggattgg tcaaatgcgc caagcaggtt
     1561 taacagtggc tgcatcgctt agcccgcaac gaacagcgca atatgcatcg caagttgttt
     1621 cagcaggtgt agatatattt gttattcgcg gtacaacagt aagtgccgag catgtctctg
     1681 ctggatcaga gccacttaac ctcaaagaat ttatctataa attagatgtc ccagtgatag
     1741 ttggtggttg tgccacatac accgcagcac ttcacttaat gcgaactggc gctgctggcg
     1801 tattggttgg ttttggtggt ggcgcggcac acacaactca agatgtactt ggcgttcggg
     1861 tgcctatggc atcagccgtg gcagatgttg cagccgcaag acgcgattat cttgatgaat
     1921 ctggtggcag atatgtttca gtaattgccg atggatcgat gggacatagc ggtgatgtgg
     1981 ctaaagcgat tgcattagga gcagacgcgg ccatggtcgg ctcaatattt gctagagcta
     2041 ctgatgcacc aggccaagga aaacactggg gtctggaagc tacccatcca gatttaccgc
     2101 gaggcgagtt ggttgagttg ggtacatccg gaactttgac tgaaattgtt aacggtccat
     2161 cgaatgcaac agacggcacg atgaacttag ttggcgcatt aagaaggtcc ttagcaactt
     2221 gtgggtattc agacctaaaa gaattccaac gctgcgaagt cgtggtaacc ccacgctaat
     2281 aacaaatttc tagttagctt cgctcaagat cagttgagca tttaggctag tgctgtgctg
     2341 acaactggtg aggttcaact catggataac gtggttgcaa ctagtgggga ttcagtaact
     2401 ccaattagtc cctttcctgg acaaactatc caaaccgtcc cactttcatc aatagatgat
     2461 gtgaaggtag cagttgcgca agcccgagca gcaagtgaaa cttgggctaa aactagtatt
     2521 gaagatcgga taaagatttt tgatcgttat tacgaactgc tttttgaaaa ccaagaatta
     2581 ttacaagacg taattcaaaa ggaaaccggt aaagcaaggt cgcatgcggc cgctgaaatt
     2641 tttggtgttg ccatgatcgc tgcacattac gcgaaaattt caaaaaaagc attaagccca
     2701 gaacgtcggg taggaatttt tccggtactt acaaaaacaa aagttttagc acaaccaaaa
     2761 ggtgtggtgg gaattatttc accatggaac tatccgttga cactttcgat ctatgacgcc
     2821 atacctgcaa tgttggctgg taatacagtc gtgatcagac cagattttca aacggcttgg
     2881 tcagcaattc atgcagttga tttgttgaca caggccggtt taccaaaggg tgttgccact
     2941 ttagtcatcg gtgatggccc aactgtgggc gccgcgctaa ttgatgaagt tgattacatt
     3001 tgcttcaccg gatctactgc tacgggcaga aaagttgggg ctcaagccgg ggctagatta
     3061 attggtgctt cgctcgaatt gggtggcaaa aatccgatga ttgtctgtaa ggacgctaat
     3121 ctaaatactt ttcttgagtc agcagtccaa ggatgtttta cttcagctgg gcagctttgt
     3181 atatcaattg aacgcatgtt tattcatgaa gatatttatg aagaattttt aacggccctc
     3241 gtagctcgag ttaaaaaaat tcagctaggt gccagtttga cttgggatta cgaagttggc
     3301 tcccttggta gtgaagctgt attaaatcga gtagaaaaag cagttgcgac tgcactttcc
     3361 gaaggcgccg ttgctgttgt cggcggcaag agtcgacctg atatcggacc tttcgtatac
     3421 gaaccaactg tattaactgg tgtcactaaa gatatgaaga tttatcatga agaggttttt
     3481 ggcccggttg tttacgtact cccattcaaa gacaatgaag aagctattcg aatggctaat
     3541 gattctgaat acggtttatc tgcctcagtt atttcgcgtg acttagatca agctgcacaa
     3601 attgcgtcgc gtattagaag tggttcagta aatgtaaatg atggttttgc tgccgcattt
     3661 ggatcggtcg ctgcacctat gggtggcatg ggtcaatctg gattagggcg tcgacat
//
LOCUS       DBNHVN010000240         3090 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1679722, whole genome shotgun sequence.
ACCESSION   DBNHVN010000240 DBNHVN010000000
VERSION     DBNHVN010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1679722"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..739)
                     /locus_tag="AC16Y1_04525"
     CDS             complement(71..739)
                     /locus_tag="AC16Y1_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5831760.1"
                     /translation="MLRLLLVDSDVKLVEPLKKVLNSDGYAVAHVTNGLTALEKIKLD
                     PPDLMLVDANLPEVNGFEVCETLRKQGFDFPIVMISSNFECSEIVHGFESGADDYISV
                     PFDKSELLARLRAICRRSLKSDSTSLCIGKVKLDRKSHICIVDDYQIDLTRIEYSLLD
                     YLMQNEGRAVKRQLIIREIWATSWLGPTKNLDMHISTLRRKLGPGAAQLKTVRGIGFR
                     FDSV"
     gene            complement(749..1552)
                     /locus_tag="AC16Y1_04530"
     CDS             complement(749..1552)
                     /locus_tag="AC16Y1_04530"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR5831761.1"
                     /translation="MDSIQAKSGYSKLDISRLSSLIDQSGWLKPLIFETVTSTNDIAI
                     ANLEFLDSVLGIVVVADEQTAGRGRLDRKWVAPAGSGIAMSIGTKTQNFSAQFSAVPL
                     MCGLAVHRALKAFGISTYLKWPNDVVFVNPNKNDLRKVGGVLVQLVQDSFVIGIGLNV
                     DLKSEDLPVPQATSLHLEGFEVSREDIISKVLEEINFLQHSDNLWIDDYSKVCVTLGK
                     KVSVTNFSDQTVTGMAQKVLPSGALVVKNLENLYQITVGDIVHLEVESS"
     gene            1589..>3090
                     /locus_tag="AC16Y1_04535"
     CDS             1589..>3090
                     /locus_tag="AC16Y1_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004949090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR5831762.1"
                     /translation="MGTEKSFDIHTTAGKIADLKNRREQSSTRRKESVDKQHAKGKLT
                     ASERLERLLDEGSFQQLDGLARHRSHNFGMQKSRPYGDGVITGYGTIDGRPVCVFAQD
                     FTVFGGSLGEVFGEKICKVMDLALKTGVPIIGLNDSGGARIQEGVVSLGLYAEIFKRN
                     THASGVIPQISLILGPNAGGAVYSPALTDFVIMVDKTSHMFITGPDVIKTVTGEEVEF
                     EDLGGARTHNTKSGVAHYMAHDEDDAFDYVKALLSYLPSNNMDEVPTIDVDSIMEVTD
                     HDRELDTLIPDSPNQPYDMHKIIEIILDDDDFLETQPLFAPNIITGFGRVDGQPVGVV
                     ANQPMQFAGTLDIDASEKAARFVRTCDAFNVPVLTFVDVPGFLPGTDQEWQGIIRRGA
                     KLLYAYAEATVPLITVITRKAYGGAYDVMGSKHLGADLNLAWPTAQIAVMGASGAVNI
                     LYRKEIAEATDPQAERVRLMAEYDDKFANPYLAAERGYVDRVIYPHETRV"
BASE COUNT          884 a          660 c          661 g          885 t
ORIGIN      
        1 tgggtattcc cggaagaaac tgttctacca attgattaag taaggcacct agttgttagc
       61 gctagttgcc tcaaactgaa tcaaagcgaa atccgattcc tcgaactgtt tttaattgcg
      121 cggcgccagg gccaagtttt ctgcgcaaag tagaaatatg catatctaaa ttttttgtcg
      181 gacctagcca agaagttgcc cagatttccc tgatgattaa ttggcgcttg actgctcgtc
      241 cttcgttttg cattaaataa tcaagcaacg agtactcgat tcgtgttaaa tcgatttgat
      301 aatcatcgac tatacatatg tgagactttc ggtctagctt cacttttcct atgcacagtg
      361 aagtggaatc agacttaagt gaacgtcgac aaatagcccg cagccttgct aacaattcag
      421 atttatcgaa aggaacagaa atgtaatcat cagcaccact ttcaaaacca tgcacaattt
      481 ctgaacattc aaaattggag ctaatcatca caattggaaa atcaaaacct tgttttctta
      541 atgtttcaca aacttcgaaa ccatttactt ctggcaaatt tgcgtctacc aacattagat
      601 cgggagggtc taatttaatt ttttctaatg cggttaaccc gttagttaca tgtgcaactg
      661 catagccatc tgagttgagt accttcttta atggttcaac caacttaacg tcactatcga
      721 ctagcaaaag tcgcaacata aagttagatt aactcgactc cacttccaga tgcactatgt
      781 cgccaactgt tatttggtaa agattttcta aattttttac taccaatgca ccggacggta
      841 aaactttttg cgccataccg gttacggttt gatctgaaaa atttgtcaca gaaacttttt
      901 taccgagtgt tacacatact tttgaatagt catcgatcca caaattatct gaatgttgta
      961 aaaaatttat ttcctctaga actttgctga ttatgtcctc acggctgact tcaaacccct
     1021 ctaggtgcag tgacgttgct tgcggtactg gaagatcttc tgactttaaa tcgacgttta
     1081 atccgatgcc gatcacaaaa gaatcttgga cgagttgaac taaaactccg ccaactttac
     1141 gtaagtcatt tttattcggg ttgacgaaaa ctacgtcgtt tggccatttc aagtaggtgc
     1201 taatgccaaa tgcctttaat gctctatgaa cagcaaggcc acacatcaga ggtaccgcag
     1261 aaaattgcgc tgaaaagttt tgtgtttttg tgccgattga cattgcaatg ccgcttccag
     1321 ccggtgcaac ccactttcga tcaagcctgc cccgcccagc tgtttgttca tccgcaacta
     1381 caacaatgcc aagcacggaa tctaaaaatt caaggtttgc tattgcaata tcatttgtgg
     1441 aagttaccgt ttcgaaaatt aagggtttaa gccatccact ctggtcaatt agtgaactca
     1501 atcgcgaaat atcaagtttt gaatagccac ttttggcctg aatactgtcc acatcgctag
     1561 ggtaggcgaa gagggcacgg agggaatagt gggcaccgaa aagtcttttg acatccatac
     1621 aactgcaggc aagattgctg acctaaaaaa tcggcgtgag caatcatcaa ctcgtcgtaa
     1681 agagtcagtc gacaaacagc atgcaaaagg caagctaact gcatcggagc ggctagaacg
     1741 tttgcttgat gagggttcgt ttcaacaatt agatggctta gccagacacc gctctcataa
     1801 ttttggaatg caaaaatcgc gaccttatgg cgatggtgtt atcactggtt acggaacaat
     1861 cgatggtcgg ccagtctgtg tgttcgcgca agacttcacg gtatttggtg gatcacttgg
     1921 cgaagtcttt ggcgaaaaaa tatgcaaagt tatggatctg gctttaaaaa ctggtgtgcc
     1981 aattatcggc ttaaatgatt ctggtggtgc tcgcattcaa gaaggtgtgg tttcacttgg
     2041 actttacgca gaaattttca agcgcaatac acatgcttct ggggtaattc cgcagatctc
     2101 tttgattctc ggtccgaacg ccggaggtgc ggtttattct ccagccttaa ctgactttgt
     2161 gatcatggtt gataaaactt cacatatgtt catcactggg cccgatgtaa ttaaaacagt
     2221 taccggtgaa gaagttgagt ttgaagattt gggtggtgca agaacacata atactaagtc
     2281 aggtgttgca cactacatgg ctcacgatga agatgacgcg tttgattatg taaaggcatt
     2341 actgagttac ctaccgagta acaatatgga cgaagtccca actattgatg ttgattcaat
     2401 aatggaagta accgaccatg atcgtgaact cgatacgtta attccggatt caccaaacca
     2461 gccatatgac atgcacaaaa ttattgaaat aattcttgat gacgatgatt ttctcgaaac
     2521 ccaaccactt tttgcaccaa acataatcac tggtttcggc cgggtagatg ggcaacctgt
     2581 tggtgtggtg gcaaatcaac ctatgcagtt tgcaggaaca cttgatattg atgcatctga
     2641 aaaagcagcg cgttttgtgc gcacctgtga tgcatttaat gttccagtgt tgacatttgt
     2701 tgatgtacct ggcttcttac ctggaaccga tcaggaatgg caaggaatca ttcgtcgtgg
     2761 tgcaaaactt ctttatgcat atgccgaagc aacagttccg ctcattactg taattacccg
     2821 caaagcctac ggcggcgcat acgacgtgat ggggtcaaaa catcttggcg ctgacttaaa
     2881 tttggcttgg ccgactgcgc aaattgcagt gatgggtgca tctggtgcgg tcaatattct
     2941 ttaccgcaaa gaaatagcag aggcgacaga tccacaagcc gagcgggtgc gcttaatggc
     3001 tgaatacgat gacaaatttg ccaacccata tctagctgca gaacgtggtt acgttgaccg
     3061 agtaatttat ccgcatgaaa ctcgagtgat
//
LOCUS       DBNHVN010000241         1952 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_118499, whole genome shotgun sequence.
ACCESSION   DBNHVN010000241 DBNHVN010000000
VERSION     DBNHVN010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_118499"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="AC16Y1_04540"
     CDS             complement(<1..846)
                     /locus_tag="AC16Y1_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010644393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5831763.1"
                     /translation="MSYDASAIQVLEGLDAVRKRPGMYIGSTGERGLHHMVYEVVDNS
                     VDEALAGHCTQIDVTLLTDGGVRVVDNGRGIPVGIVASEGKPAVEVVLTVLHAGGKFG
                     GGGYSVSGGLHGVGVSVVNALSKKVDVEVKTEGAIHTQSYKLGVPTAPLKKGGKTSET
                     GTSVTFWADDKIFETTVYDFETLSRRFQEMAFLNRGLTIRLTDERPGTKVITDDIEND
                     RVTDGPKSVTYKYDGGIADFVSHINARKQPIHSSVIQFFAEDTKRGISAEVALQWNST
                     YTESVY"
     gene            complement(992..1501)
                     /locus_tag="AC16Y1_04545"
     CDS             complement(992..1501)
                     /locus_tag="AC16Y1_04545"
                     /inference="COORDINATES: protein motif:HMM:NF017103.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HIR5831764.1"
                     /translation="MLTAEQQEFLRSLLSRARIKTGAKIYRAPTTQVFVDEQRSGAGP
                     DERDPAKLAGLIDNIIINKGWDLQLATGKLRARWDKIVGADVADHVVIEQFQMNASGQ
                     SGILVLRTDSTAWATQIKLLMPTLTEKLDTEVGQGRISEIKVLGPNAPSWKHGLRSVP
                     GRGPRDTYG"
     gene            complement(1505..>1952)
                     /locus_tag="AC16Y1_04550"
     CDS             complement(1505..>1952)
                     /locus_tag="AC16Y1_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014137023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR5831765.1"
                     /translation="ATLSADNEPLEINYFAKWFNDEKNIEQQFKDSLAEKQNEELDRG
                     ITLVGPHRDEVELKLSGLPAKGYASHGQSWSIALAMRLATFNVLREHDDDPILILDDV
                     FAELDQRRRTRLLASISGVEQTLITAAVAEDLPEGLAGEQILLEDN"
BASE COUNT          494 a          461 c          410 g          587 t
ORIGIN      
        1 gtaaaccgat tcggtgtatg tgctattcca ctgcagggct acttcggcgc tgatgccacg
       61 cttagtatct tcggcaaaaa actggatcac acttgaatgg attggctgtt tccgagcatt
      121 gatgtgcgaa acgaaatctg caataccgcc gtcatatttg taggtaactg atttagggcc
      181 atcagttact cgatcatttt caatgtcgtc ggttataact tttgtgccgg ggcgctcgtc
      241 cgttaatcga atagttaaac cgcgatttaa aaacgccatt tcttggaaac gtctggaaag
      301 ggtttcaaaa tcgtaaacag tagtttcaaa aattttgtca tcggcccaaa aagttacgct
      361 tgttccggtt tcagatgttt tgccaccctt tttaagtggt gcggttggca cacctaattt
      421 gtaagactga gtgtgtattg cgccttcagt tttaacttca acgtctactt ttttcgaaag
      481 cgcatttact acagacacac ctacaccgtg tagaccacct gaaactgaat atccgccgcc
      541 gccaaatttt ccaccagcgt gcagaactgt taatacaacc tcaactgcag gttttccttc
      601 tgatgcaaca atgccaactg gaataccgcg accattatca acaacgcgca caccgccgtc
      661 agttaataac gtgacatcga tttgcgtgca atgaccagct aatgcttcat caactgagtt
      721 gtcgacaact tcataaacca tgtggtgaag accgcgttca ccggtagaac cgatgtacat
      781 acccgggcgt tttcgaaccg catccaagcc ctcgaggacc tgaatggcac ttgcgtcgta
      841 tgacactaaa agggccttcc atgtgagcct atttttgggc caaaaaggct aagaaataag
      901 gcttttttgc agttttttag gtagttttat tctacctgtt ttgtgggctt agcaacaccc
      961 agatatgccc taaaaaggcc ctaaaaagcc tttatccgta ggtgtctcga ggccctcttc
     1021 caggcacact tcgcagccca tgtttccaag acggagcatt gggtccgagc acttttattt
     1081 ctgaaattcg tccttggccc acttcggtat cgagtttttc ggtaagggtt ggcatcagta
     1141 atttaatttg agttgcccaa gcagttgagt ctgtgcgcaa aaccaaaatt cctgattgtc
     1201 cagatgcatt catttgaaat tgctcgatca ccacatggtc ggcaacatct gcaccaacta
     1261 ttttgtccca tctggcgcga agttttccgg tggctaactg taagtcccaa cctttattga
     1321 taattatgtt gtcaattagc ccagctaatt ttgctggatc gcgttcatct ggtccagcac
     1381 cacttcgttg ctcatctaca aaaacttgtg tggtgggcgc gcgataaatt tttgcaccag
     1441 ttttgatgcg cgctcttgat aataaggagc gcaagaattc ttgttgttct gcagttaaca
     1501 cgatctagtt atcctctaac aaaatttgtt cgccggctag cccttcgggt aaatcttcgg
     1561 ctaccgcagc agtgatcaag gtttgttcaa caccgctaat cgaggcaagt aatcgagttc
     1621 ttcttcgctg atctaattca gcaaagacgt catccaaaat taaaattgga tcgtcgtcgt
     1681 gttcgcgcaa aacattaaat gtggcaagtc gcatggcaag agcgatactc caggattgac
     1741 cgtgcgaagc atagccttta gctggcagtc cacttagttt taattccact tcatcccgat
     1801 gtggaccaac caaggtaatt ccgcgatcta actcttcatt ttgtttttcg gctaacgagt
     1861 ctttaaattg ttgctcgata tttttttcat cattaaacca ttttgcaaag taattaattt
     1921 ctagtggttc gttgtcggca cttaatgttg ct
//
LOCUS       DBNHVN010000242         1638 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_47613, whole genome shotgun sequence.
ACCESSION   DBNHVN010000242 DBNHVN010000000
VERSION     DBNHVN010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_47613"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1638)
                     /locus_tag="AC16Y1_04555"
     CDS             complement(<1..>1638)
                     /locus_tag="AC16Y1_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014145233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR5831766.1"
                     /translation="WTLFALTVMGITHLIQGNPAPAAGAAAMRSASGWLGWLFTYPFV
                     TAVGSAVTYLLLAVLWFFSMLIITATPVASIPGKVGQLVSRFSRAEEESEAVLAHPTY
                     EAISAAQDLENIPVTKTSFFARFRRKDKNDFELDPALTIGELDGDQPFINPVVMVRPQ
                     GQELSIDQYATEAISLIEPIEDQSTVAIDVSKVDIKPVAQVAPKAKPVQLPLASALNY
                     KLPSMDHLKPGAVAKERTKANDVVIEALTQVLEQFEIDATVTGFQRGPTVTRYEVELG
                     PKVKVERVTALSKNIAYAVASDDVRILSPIQGKSAIGIEIPNTDREVVSLGDVLRSKE
                     ANADEHPMLVGLGKDVEGGFTVANLAKMPHLLVAGATGAGKSGCMNTLITSILMRATP
                     DEVRMVLIDPKRVELTAYEGIPHLISPIITHPKKAADALAWVVKEMEARYDDLAAYGF
                     RHVDDFNKAVKAGKLTPPAGSERVLRPYPYLLVVVDELADLMMVAPRDVEDSVVRITQ
                     LARAAGIHLVLATQRPSVDVVTGLIKANVPSRLAFSTS"
BASE COUNT          445 a          386 c          367 g          440 t
ORIGIN      
        1 gaagttgaga aagccaagcg acttggcacg ttggctttaa ttaagccagt aaccacatca
       61 actgatggtc gctgagttgc caaaactaaa tggataccag cggcgcgagc taactgcgtt
      121 atgcgcacaa ctgaatcttc aacatcacgc ggtgccacca tcatcaagtc ggctaactca
      181 tcaacaacca caagcaaata tggataagga cgaagtaccc gctctgaacc ggcaggtgga
      241 gttaacttgc cagctttaac tgctttattg aaatcatcaa catgtcggaa tccataagct
      301 gctaaatcgt cgtagcgcgc ttccatttct ttaaccaccc aagcaagtgc gtcggcagct
      361 ttctttggat gcgtaatgat tgggctaatt aaatgtggaa taccttcgta agctgttaat
      421 tcaacgcgct ttggatcgat caaaaccatg cgaacttcgt ctggagttgc gcgcatcaaa
      481 atcgaggtga tcaacgtatt catacagcca gacttgcccg caccagttgc gcccgcaaca
      541 agtaagtgtg gcatcttggc taagttagca acggtaaagc caccttcaac atctttacct
      601 aggccaacca acattgggtg ctcatcggca ttagcttctt tgcttcgtag cacatcacct
      661 agtgaaacta cttcacggtc tgtatttgga atttcgatac cgattgcact cttaccttga
      721 attggcgaaa gtatgcgcac atcatcactt gctactgcat atgcgatgtt cttgctaaga
      781 gcggtaacgc gttcaacttt aacttttgga ccaagttcaa cttcgtagcg ggttaccgtt
      841 gggccgcgtt ggaacccggt taccgtggcg tcaatttcga actgttcaag tacttgggtt
      901 agcgcttcga taaccacatc atttgcctta gtgcgctctt tagcgacggc gcctggcttc
      961 aagtgatcca tcgatggaag tttgtaattt aatgcgctgg ctagtggaag ttggactggc
     1021 tttgccttag gtgctacttg agcaactggc ttaatatcta ctttagaaac gtctattgca
     1081 accgtcgact gatcttcgat tggttcaatc agcgatattg cttctgttgc gtactgatca
     1141 attgataact cttggccttg tgggcgcacc ataacaaccg gattaataaa tggttgatcg
     1201 ccatcaagtt caccaattgt aagtgctggg tctaactcaa agtcattttt atctttccgg
     1261 cggaagcggg caaaaaatga tgttttagtt actgggatat tttctaagtc ttgagcagca
     1321 gaaatagctt cataagttgg gtgcgcaagt acggcttcag attcttcctc tgctctactg
     1381 aaacgagaaa ctaattgccc aactttgcct gggattgatg caaccggtgt agcggtaata
     1441 atcaacatag agaagaacca taaaactgca agtagtaaat aagtcacagc agagccaact
     1501 gcagtaacaa acggatacgt aaataaccag ccaagccagc cacttgcact tcgcattgca
     1561 gcagcgccag ctgccggtgc tggattacct tgaatcaaat gggtaatgcc catcacagtt
     1621 aacgcaaaaa gtgtccag
//
LOCUS       DBNHVN010000243         1758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1977896, whole genome shotgun sequence.
ACCESSION   DBNHVN010000243 DBNHVN010000000
VERSION     DBNHVN010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1977896"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="AC16Y1_04560"
     CDS             <1..464
                     /locus_tag="AC16Y1_04560"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009482434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HIR5831767.1"
                     /translation="ELVDPINRIFLPRADIATDTLVAGLTELGWEVEDVTAFRTVRAA
                     PPAVETREAIKSGGFDAVVFTSSSTVRNLVGIAGKPHPASIIACIGPATAKTAQEHGL
                     RVDVLPEDASIHALTQALAAHGYALREEAAKAGVVGWRPSRKRSASRRRAT"
     gene            479..1114
                     /locus_tag="AC16Y1_04565"
     CDS             479..1114
                     /locus_tag="AC16Y1_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015796631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR5831768.1"
                     /translation="MRTTVHLLRHGEVHNPEKVLYGRLPGYVLSDLGHEMAKRASIAL
                     AHRDIAAVISSPLERAQQTATPLAQVHKLDILLDENLIEAENVFEGQRVSVGDGVLRQ
                     PRAWKHLYNPVKPSWGEPYKVVASRMRKAVTKARELAPGREVVLVSHQLPIWISRLDF
                     EGRRFVHDPRRRQCNLASLTSLVFDDNELSAVLYTEPSADLIVKASKIAGA"
     gene            1107..1667
                     /locus_tag="AC16Y1_04570"
     CDS             1107..1667
                     /locus_tag="AC16Y1_04570"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HIR5831769.1"
                     /translation="MRKLLIALLTIPFLVACSSSDGSVTGSETSFVAGDGTMTLVPAV
                     EREQPVELSGITLEGEPLDLVDYRGKVVVVNVWASWCAPCRAEADELANLAKEFSNES
                     VQFIGLNTRDSLPSAIAFNERFATGFPSIQDKEGELTLAFGKLGPAATPTTIVLDKSG
                     QPAGRILGVITEAQLRNLIKAILEEA"
BASE COUNT          476 a          393 c          433 g          456 t
ORIGIN      
        1 atgaattagt cgatccgatc aatcgaatct ttttaccgcg agcagatatc gcaactgaca
       61 ctctcgtcgc cggtttaact gagcttggtt gggaagtaga agacgtaaca gcattccgca
      121 cagtacgcgc tgcacctcca gctgtagaga ctcgtgaagc aattaagagt ggcggttttg
      181 atgccgtggt attcacttca agtagtaccg ttcgcaacct tgttggtatt gcaggtaagc
      241 cgcatccggc aagcatcatc gcttgtatcg gaccggcaac agccaagact gcgcaagaac
      301 atggattacg agttgatgtt cttccagaag atgcatcaat tcacgcactt acccaagcac
      361 tagctgcaca cggttacgca cttagagaag aagctgccaa agcaggagta gttggttggc
      421 ggccatctcg taagcgttca gcttcacgtc gtcgagcgac ttagtaatga gttcccagat
      481 gcgaaccaca gttcacctac tgcgccatgg ggaagttcat aatccagaga aagtccttta
      541 tggccgctta cccggctatg tactttctga cttaggtcat gaaatggcaa aacgagccag
      601 cattgcgttg gcacatcgag atatcgcagc ggtaattagt tctccgctag agcgcgcgca
      661 acaaacggca acgccgttag cacaagtaca caaactcgat attttgttgg atgaaaattt
      721 aattgaagct gaaaatgttt ttgagggtca acgagtttca gttggtgatg gcgtattaag
      781 acaaccacgc gcctggaaac acttgtataa cccagtaaaa ccctcttggg gcgaaccata
      841 caaagtagtt gcaagtcgga tgcgaaaagc agtaactaaa gcaagagaac tcgccccagg
      901 ccgcgaagtg gtgctggtta gtcatcagtt accaatttgg attagccgct tagattttga
      961 aggccgacgc tttgtacatg atccgcgtcg tcgtcaatgt aatttggcct cgctgacttc
     1021 gctggttttc gacgataatg aattatctgc agttttatac accgaaccat ctgcagactt
     1081 aattgttaaa gcgagcaaga ttgctggtgc gtaaattact catcgcatta ttgaccattc
     1141 catttttagt tgcctgcagt agttcagatg gctcagtaac tggaagtgaa acaagctttg
     1201 tagccggcga tggcacgatg accttggttc cagcagtaga gcgtgagcag ccagtcgagt
     1261 tatctggaat cactcttgaa ggagagccac tcgacttggt cgattaccgc ggaaaagttg
     1321 tcgtagtaaa tgtttgggcc agttggtgcg caccttgccg agcagaggca gatgaacttg
     1381 cgaacttggc aaaagaattc tctaatgaaa gtgtgcaatt catcggctta aatactcggg
     1441 atagtttgcc atccgcaatt gcctttaatg aaagatttgc tactggcttt ccttcaattc
     1501 aagacaaaga aggcgaacta actttagctt ttggaaaact tggtccagct gcaactccga
     1561 caacaattgt gttggataaa tcgggtcaac cagcgggtcg aattttaggc gtgataaccg
     1621 aagctcaact gcgcaactta atcaaagcaa tactcgagga agcgtagtca tgcagctagt
     1681 ttcgggtgtg acggaaattg taagtaacgg ttcactgtta cttgcatttc cacttgcttt
     1741 tgcggcaggt gtaatttc
//
LOCUS       DBNHVN010000244         6224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_638699, whole genome shotgun sequence.
ACCESSION   DBNHVN010000244 DBNHVN010000000
VERSION     DBNHVN010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_638699"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="AC16Y1_04575"
     CDS             <1..937
                     /locus_tag="AC16Y1_04575"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5831770.1"
                     /translation="QFMEFFDALPAGLMQCITGGAAAGQLLVSSPKTDMIAFTGSVPT
                     GRAVVKACAETFKPHMIETSGNDPFIVMPSADLSIAARGVAFAAFLNAGQVCTSAERI
                     YVHEEIHDEFVSLLVAEAKGIRVGNGLEKVDIGPMVNQRERDRYEKILARAIQQGAHV
                     ECGGGRPSGLDQGFFVEPTVLTSVKPEMDILHGESFGPVAPICKVSSLDEAIDLANDS
                     DFGLGANIYTMRLDEAMRAVNEIEVGMVWVNAPLLDNDAGPFGGRKLSGSGRQLGREG
                     LDSFRHTKLAMIDPEARPEDFWWFPYKDDESFQGN"
     gene            981..1154
                     /locus_tag="AC16Y1_04580"
     CDS             981..1154
                     /locus_tag="AC16Y1_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831771.1"
                     /translation="MPELNLPAELEERIRYYEDPNNDPGGFATKDWAVVIGSGIVLPL
                     VCILIGWNFGWPS"
     gene            1151..2548
                     /locus_tag="AC16Y1_04585"
     CDS             1151..2548
                     /locus_tag="AC16Y1_04585"
                     /inference="COORDINATES: protein motif:HMM:NF014218.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-cytosine permease family protein"
                     /protein_id="HIR5831772.1"
                     /translation="MSEKTLDQELTETAWPLTDKERNWNGRSLFRVLMVAAAATWCYV
                     IGEYVGYYLPLWPGTFAMIAGAMIGMLLVTLAVVPTSTRFGIDSIKAAVPQFGTNGWT
                     ITVSLQYLSIIGWNALLLIFFGKSVSQFLNVLGVGDENLANVVVPGATLVACLGVYFI
                     LKRGVTGLEKVALWLFFSIIAVGAWLIIMLAIRQGDTLATALPAYASDKQLDFQYGIE
                     IALVSLLSWWPYIGSMTRLARNPSIAAGPSMLGMGLPVPILSFVGLASILALGISDPT
                     AWLTEVGGQFFGALALLFIIAANLGTALAGLYAATVGIKAVPAFAKLSWNRALLVSIL
                     PVAAIGLFIPNWVFDNFGTFVAYIGVFFAPLCGIMIVDYFILRKGKISIRGIYETGNK
                     SVYRYWGGFNPAAVLAMVVGFFTYTYLLDPISYEIKNPFPLVGASLPAALLSALSYLL
                     VTVLIVKPSGKGGYK"
     gene            complement(2616..3839)
                     /locus_tag="AC16Y1_04590"
     CDS             complement(2616..3839)
                     /locus_tag="AC16Y1_04590"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5831773.1"
                     /translation="MTPPKVVILGAGFGGLTAARALDKDADVLLIDQHNYQTFLPLLY
                     QVSTAGLAADHVAYPIRGALRKTNVKFRMGSPKAIDHQANTITLADGFEVKYDHLVIA
                     LGSTTADFGVPGVTEHALGMKTVSEALHIRSKVMLAYEKMCAANDAHDFEISVVGGGP
                     TGVEMAGAFAELIRGPLKSDFAQVASRFKVSIIEAGPRLLPSFAPSLAQKTKEDLEKL
                     GVAVLTNTVIKEVKSDALLTDKELVIPAKLTVWAAGVKGVSVIDQLGLPTNRGRLVVD
                     PTLQVSGHKNIWAIGDVAGVQDSSGNLLPMVAPVAMQQARFLAKQISSSKSGQLLKSF
                     KYRDKGSMATIGRHKAIVEVKGIKLSGPIAWLAWLWLHLFYILGGRNKIGTMADWTWN
                     YLTFDRSNRHIMDSL"
     gene            complement(3919..4797)
                     /locus_tag="AC16Y1_04595"
     CDS             complement(3919..4797)
                     /locus_tag="AC16Y1_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831774.1"
                     /translation="MITGFQLDSKNKPIPVGLRIILTLSSPGEKTVTFSISSDGEIEN
                     YLQKLTKINPNFGDEIWIEPTPESKSQRFIVHAPADQRILLGDFPIQISFEITVERKF
                     SNQWMTTNKVLIILNDNQHKIFQAREFTESYQKNDLNSVEELNDESSTATETLLSGET
                     QTHAASEISGPLNQSTKARKKFSRPIRLVAGLSLILPAVFLLTPLSLFALDQPDDRNA
                     KLAITLPATESAIGETIVALLVDQNGNAESYLGTVENKSTTAYLLRTGDNFIQVEKDQ
                     VQGRVIISFPFVGLPF"
     gene            complement(4872..4955)
                     /locus_tag="AC16Y1_04600"
     tRNA            complement(4872..4955)
                     /locus_tag="AC16Y1_04600"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4919..4921),aa:Tyr,seq:gta)
     gene            5173..5664
                     /locus_tag="AC16Y1_04605"
     CDS             5173..5664
                     /locus_tag="AC16Y1_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HIR5831775.1"
                     /translation="MADSSMDIVSKVDRQEADNALSQAAKEIATRFDFKGTDTKIEWK
                     GELGIEITSSTEDRAKAALDVFKEKLVKRGVSLKALNSGDPRVSGKEVKINGDFKAGI
                     NQEQAKKISKIIRDDGPKNVKTQIQGEELRVTGKSRDDLQEVQQLLNSSELDFPVQYT
                     NYR"
     gene            complement(5665..6060)
                     /locus_tag="AC16Y1_04610"
     CDS             complement(5665..6060)
                     /locus_tag="AC16Y1_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5831776.1"
                     /translation="MEHKSIALHPASCTSCMLCVRECPDWCISIEAHSEPDPDATQYS
                     NARRPTTKNVLDKFTINFGDCMWCGICVEVCPFDALFWSDTPIEAAGSAADLVLDQTK
                     LVNFLPGAKRSDGTSAPQLIDPPSGRRKK"
BASE COUNT         1728 a         1333 c         1363 g         1800 t
ORIGIN      
        1 acagttcatg gaattttttg atgctcttcc agctgggctt atgcaatgta taactggtgg
       61 cgctgctgct ggtcagttac tggtatcgag tcctaaaaca gacatgattg ctttcactgg
      121 aagcgtgcca actggacgtg cggttgtaaa agcctgtgca gaaacattta agccgcacat
      181 gattgaaaca tcgggcaacg acccatttat agtgatgcca tcagctgatt taagtattgc
      241 tgcacgtggt gttgcatttg ctgcattttt gaatgctggc caagtgtgta cgtcagcaga
      301 acgcatctac gttcatgaag aaatccacga tgaatttgtc tcgcttcttg tcgctgaagc
      361 aaaaggtatc cgagttggaa atgggcttga gaaagtagac attggcccga tggttaacca
      421 gcgggaacga gatcggtacg agaaaatatt agctcgagct attcagcaag gtgcacatgt
      481 tgaatgtggt ggtggtcgtc caagcggctt ggatcaaggc ttctttgttg aaccaacagt
      541 attaacgagt gtgaagcccg aaatggatat tttgcacggt gagtcattcg ggccggttgc
      601 gccaatctgt aaagtatcca gtcttgatga agcaattgat ctagctaatg attctgattt
      661 tggtttaggc gcaaacattt acacaatgcg ccttgacgaa gccatgcgcg cagttaatga
      721 aattgaagtt ggcatggttt gggttaatgc cccactactt gataacgatg caggaccatt
      781 tggtggccgc aaactttcag gttcgggtcg ccaattaggc cgtgaaggcc tggacagttt
      841 ccgtcacacc aaacttgcca tgatcgatcc cgaagcaaga ccagaagatt tttggtggtt
      901 cccttataaa gatgatgaaa gcttccaagg taactaggag gaaaaatgac ccgagcaaca
      961 tcaaatgaaa ggaaaacggt atgcccgaac taaatcttcc agctgagctg gaagaacgta
     1021 tcaggtacta cgaagatcca aataacgacc caggtggttt cgccaccaaa gactgggcgg
     1081 tagtaattgg ttctggcata gtgctaccac ttgtctgcat tcttattggt tggaattttg
     1141 ggtggccatc gtgagcgaaa aaacattgga tcaagagtta actgaaaccg cttggccgct
     1201 aaccgacaaa gaacgtaact ggaatggtcg ttcactattc cgagtactta tggtcgctgc
     1261 agcagctact tggtgttacg taatcggtga atatgtcggc tactacttac cactttggcc
     1321 aggcactttt gcaatgattg ctggagccat gatcggtatg ttgcttgtga cattggcagt
     1381 tgtgccaacg tctactcgct ttggtatcga cagcataaaa gctgcagtgc cccaattcgg
     1441 tacaaacgga tggactatta ctgtttccct tcagtacctt tcaattattg gttggaatgc
     1501 acttctgctt atcttcttcg gtaaatccgt ctctcaattc ttaaatgttc ttggagttgg
     1561 agatgaaaac ctagcaaatg ttgttgttcc gggcgcaaca ttagttgcct gcctcggagt
     1621 ttatttcatt ctaaaacgag gagttactgg acttgagaaa gttgcgctct ggctattttt
     1681 ctcaatcatt gcagttggtg cttggctaat tatcatgttg gcgatccgcc aaggtgacac
     1741 attggctact gcgctacctg catatgcaag tgataagcaa cttgatttcc agtacggaat
     1801 tgaaattgca cttgttagtt tgctgtcttg gtggccatac attggctcga tgactcgtct
     1861 agctagaaat ccttcaattg ctgcaggtcc atcaatgtta ggtatggggc ttccagttcc
     1921 aatacttagt tttgttggat tagcctcaat tttggctctt ggtatttcag acccaaccgc
     1981 ttggttaacc gaagtaggtg gccaattctt tggcgccctt gctttgttat tcatcattgc
     2041 ggcgaacctg ggtaccgcac ttgctggcct ctacgcagcg acagtcggta ttaaagcagt
     2101 tccagcattt gccaaactgt cctggaatag agcgcttttg gtttcaatcc ttccggttgc
     2161 agccattggt ctattcatcc caaattgggt atttgataac ttcggcacat ttgttgctta
     2221 cattggagtt ttctttgccc cactttgtgg aatcatgatc gtggattact tcatccttcg
     2281 taaaggaaag atcagtattc gcggcattta tgaaactgga aacaagtcgg tctaccgtta
     2341 ctggggtggg ttcaacccag cagctgtact agctatggtt gttggtttct ttacctacac
     2401 ctacctactt gatccaattt cgtatgaaat caagaatcca ttcccattgg ttggcgcttc
     2461 attgccagct gctctactgt cggcactaag ttacctgcta gtaactgtgc ttattgtaaa
     2521 gccttcgggt aaaggcggtt ataaataaat aaccccgggt aaaaaacaaa agcaccgaga
     2581 aatcggtgct tttgtttttt tagatgtaaa taaatctaga gcgaatccat aatgtgtcga
     2641 tttgatcgat caaaagttaa gtaattccat gtccagtcag ccatggttcc aattttgttt
     2701 cggccgccca agatgtaaaa caagtgcaac cataaccaag caagccaagc aatcggacca
     2761 gataacttaa ttcccttaac ttcgacaatg gctttatgcc ggccgatggt ggccatcgag
     2821 cctttatctc gatatttaaa actctttaac aactgccctg atttgctcga acttatttgt
     2881 ttagctaaaa accttgcttg ctgcattgca actggtgcca ccatcggcag taaattccca
     2941 gaggaatctt ggactccagc cacgtcacca attgcccaga tatttttatg accactaact
     3001 tgaagtgttg gatcaacaac taatcggcct cggttggtag gtagaccgag ttgatcaatg
     3061 actgaaacgc ctttaacacc tgctgcccat accgttaatt ttgcaggaat aactaattct
     3121 ttatcagtta aaagcgcatc tgacttaact tcttttatta cggtattagt taaaactgct
     3181 acgccaagtt tttctaaatc ttctttcgtt ttttgagcca gagatggtgc aaatgatgga
     3241 agcaatctag gcccagcttc aataatcgaa actttaaagc gactagcaac ttgtgcaaag
     3301 tctgatttca acggtccgcg aattaattct gcaaaagcac cggccatctc aactccagtt
     3361 ggtccgccac cgacaactga aatttcaaaa tcatgtgcat cattggctgc gcacattttt
     3421 tcgtaggcca gcatcacttt cgagcgaata tgcagcgcct cacttacggt cttcattcct
     3481 aacgcatgtt cagtaactcc gggcacacca aaatcagcag ttgtagaacc taaagcgata
     3541 actaaatggt catacttaac ttcaaaaccg tctgccaacg taattgtgtt tgcttgatga
     3601 tcaattgctt taggtgaacc catccgaaac ttcacatttg tcttgcgcag tgcgcctcga
     3661 attggatagg ccacatgatc tgcggctagc ccagccgttg aaacttgata aagcaatgga
     3721 agaaaagttt ggtaattatg ttgatcgata agtagaacat cggcgtcttt gtctaaagct
     3781 ctagccgcgg taagtccacc aaaaccggca ccaagaatga caactttggg tggagtcact
     3841 gtgcctcaat tcatagttaa ctaagaccct aaggttatcc cgttcaagcc aaaaccgctc
     3901 agcaagaaat ttagttggct aaaaaggtag cccaacgaat gggaatgaaa taatcactcg
     3961 accttgaact tggtcttttt ctacctggat aaaattatca ccagttcgta acaggtatgc
     4021 ggtagttgat ttattttcca cggtacccaa gtagctttcg gcatttccat tttgatcaac
     4081 tagaagtgca acaatagttt caccgattgc tgactctgta gctggcaatg tgatggctaa
     4141 tttggcattt ctatcatcag gctgatctaa agcaaacaat gacaatggcg taagtagaaa
     4201 taccgccggc aaaattaggc ttagtcctgc aacaaggcga attggccgtg aaaacttctt
     4261 gcgcgcttta gttgattggt taagcggacc gcttatttca gaagcggcat gggtttgagt
     4321 ttcacctgat aacaaagtct cggtggcagt cgaactttca tcattcaatt cttcaactga
     4381 gttcaaatca tttttttgat aactttcagt gaattcgcgg gcttgaaaaa ttttatgctg
     4441 attgtcatta agaataatta gcactttatt agtggtcatc cattggttac taaattttcg
     4501 ttcgactgtt atctcaaaag aaatttgaat tggaaaatct ccgagtagta tccgctgatc
     4561 cgcaggagca tgaacgatga agcgttggct tttactttct ggggtgggct caatccaaat
     4621 ttcatctcca aaatttggat ttattttggt taatttctga aggtaatttt cgatttctcc
     4681 atctgaagaa attgaaaaag ttacagtctt ttcaccagga ctacttaaag tcagaatgat
     4741 tcgtagacca accggtattg gtttattttt tgaatctagc tggaatccgg taatcactta
     4801 taacaatcct ggacggattt tccccgcttg tcctcaaaca ccttaaatca atagtaaggc
     4861 tgagaattaa gtggtggcag gtagtggatt tgaaccactg aagcttacgc gacggattta
     4921 cagtccgctc ccattggccg ctcgggcaac ctgccatgct tcctataaat actgtgagtg
     4981 agttaccgag ggtagattac cgagggtaaa tactgtgagt aaaaatagac aactccacga
     5041 gaataccgat aaatcaagga aaatcccaat cgttgattgg ccatggaatc gcctactatc
     5101 acctcttata tagtcagtat tcaaagtcag taaaaatttc agtactaaaa acaaagtcaa
     5161 agtgaggacc tcatggccga ttcaagtatg gacattgtta gcaaagtcga tcgtcaagag
     5221 gcagataatg cactcagtca ggcagcaaaa gaaattgcaa ctcgctttga tttcaaaggt
     5281 accgatacca aaattgaatg gaaaggtgaa cttggaattg aaatcacctc ttccactgaa
     5341 gatcgtgcaa aagctgcatt agatgtcttc aaagaaaaat tagttaagcg cggtgtcagt
     5401 cttaaggcgc taaattccgg tgacccacgc gtttcaggta aagaagtcaa aatcaatggt
     5461 gactttaaag ccggaatcaa tcaagaacaa gctaaaaaaa ttagcaaaat aattcgcgat
     5521 gacggtccaa aaaatgtgaa aacccaaatc cagggtgaag aattgagggt tactggcaag
     5581 agtcgtgatg accttcaaga agttcagcag ttacttaatt cgtcagaatt agattttcca
     5641 gttcagtaca cgaactaccg ttaattattt tttcctgcga ccacttggcg gatctattaa
     5701 ctgcggtgcg ctcgtcccat cagatctttt cgcacctggt aaaaaattaa ctaatttagt
     5761 ttgatctaaa actaaatctg cagcacttcc tgcagcctca attggtgtat cagaccaaaa
     5821 gagtgcatca aatggacaga cctcaacaca aattccacac cacatgcagt caccaaaatt
     5881 tatggtgaat ttatctaaaa catttttagt tgttggtcgc cgagcgttgc tgtattgagt
     5941 tgcatccgga tctggttcgc tgtgtgcctc aattgaaata caccaatctg gacactcgcg
     6001 aacacaaagc atgcatgatg tgcatgatgc tggatgcagc gcaatacttt tgtgttccac
     6061 tatgaattac tccaggtttc gtgaacaccg ggcggcaaac ttggtcggcg cctggctttc
     6121 tcatcaggtt cgctagcccc tggccaattt gtttgttgcc gagtagttaa ggcgaaatcc
     6181 ttgcgcaatg gatacccgga aaaatcaact tcaaatgctg gttt
//
LOCUS       DBNHVN010000245         2969 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_566287, whole genome shotgun sequence.
ACCESSION   DBNHVN010000245 DBNHVN010000000
VERSION     DBNHVN010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_566287"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /locus_tag="AC16Y1_04615"
     CDS             complement(<1..836)
                     /locus_tag="AC16Y1_04615"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR5831777.1"
                     /translation="MTDQQGNEQPAVVPAGPPTWIYSSVDTRPPTVKSDRKPSLIKTA
                     VLSGVISGLLAGTAGFIGASLINFQPNSPISLPAKAGDTSPRPNDSIAGIASAVLPTV
                     VSISVKSDSASGTGSGFIIQSNSTESYILTNNHVATGAGASAKIVISFQDQTEVPATI
                     VGTNASYDLAVLRVARGNLPVAVLGNSDDVVVGDTTIAIGSPLGLSGTVTSGIISALN
                     RPVTAGGEGDTSFINAIQTDAAINPGNSGGPLVNAAGQVIGINSAIATLGNSFSGSSG
                     SIG"
     gene            956..1609
                     /locus_tag="AC16Y1_04620"
     CDS             956..1609
                     /locus_tag="AC16Y1_04620"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR5831778.1"
                     /translation="MAPTDPRILAIHRASCDYAEGWSGEDGIRAHARAKAAEIGCVAV
                     SPGAGAVLRTFAAAIDAKNVVEIGTGAGVSALWLLEGMNPEGVLTSVDNEAEHQLIAK
                     DALTQAGISPNRVRLIYGNSQEVLDRLTDSAYDIVLISGRPLELAAHVERAMHLLSPG
                     GLLIIDRALWNDKLADSSQQDAETSAVRGMIESLATNEDLIGALLPVGGGLLVSVKK"
     gene            complement(1655..1837)
                     /locus_tag="AC16Y1_04625"
     CDS             complement(1655..1837)
                     /locus_tag="AC16Y1_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005443666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3117 domain-containing protein"
                     /protein_id="HIR5831779.1"
                     /translation="MAAMKPRTGDGPMECPKEGRGYVLRIPLEGGGRLVVELNAQEVK
                     DLGAVLKDTVAAMPKK"
     gene            complement(1959..2396)
                     /locus_tag="AC16Y1_04630"
     CDS             complement(1959..2396)
                     /locus_tag="AC16Y1_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5831780.1"
                     /translation="MEYIRLEVIVHAPVELVWNRITDWPSQSEWMLGTKVSGTGAEVD
                     GEISAFTGIGPIGFLDTMIITRWKPPHRCDVLHTGKIVKGTGSFQVERVSKNISKFIW
                     IEYLNLPFGFVGRFGFKLVRPLFVLGVQKSLDKFAKYVADFAE"
     gene            complement(2396..>2969)
                     /locus_tag="AC16Y1_04635"
     CDS             complement(2396..>2969)
                     /locus_tag="AC16Y1_04635"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR5831781.1"
                     /translation="SEYKSAYELGKKLSERKYAVITGGGPGIMEAANKGCTEGEGLSV
                     GLGIELPFEQQMNDYVELAIDFRYFFARKTMFIKYSQGFVVFPGGYGTLDELFEALTL
                     VQTKKVTSFPVVLVGSAYWTPLVDWIKNTMLRGGKISQEDVDSLHITDDLDEVVRILV
                     NAEAKRKNLVNQWGILQGTYEENPPPFGAF"
BASE COUNT          814 a          680 c          637 g          838 t
ORIGIN      
        1 ccaatacttc cgctgctacc agaaaaacta ttgccaaggg tggcgatagc agagttaatc
       61 ccgatcactt gaccagccgc attaacaagt gggccgccgg aattacccgg attgattgct
      121 gcatctgttt ggatggcatt aataaaagaa gtgtctccct cgccaccagc agtgaccggc
      181 cgattgagcg ccgaaataat tccgctggta actgttccac ttaagccaag cggagagcca
      241 atcgcaatcg tggtgtcgcc aactacaaca tcatctgaat tgccaagtac tgcaaccgga
      301 agatttccac gagctactcg caatacggcc aagtcatacg aagcattcgt gccaacaatg
      361 gtggcaggta cttcagtttg atcttggaat gaaatcacaa tttttgcact tgctccggcg
      421 ccagtagcaa cgtgattatt ggtcaagata taggactcag ttgaattact ctgaataata
      481 aaaccagagc cagttcccga tgccgaatca gatttaactg aaatagaaac aactgttgga
      541 agtactgcgc tagctatacc cgcgattgag tcatttggtc gaggtgaggt atctcctgct
      601 ttggctggca atgaaattgg tgagttcggt tgaaaattaa tcaggctcgc accgatgaag
      661 cctgcagtac cggcaagtaa tccgctaatt acaccagaca aaactgcagt tttaattagt
      721 gacggctttc gatcactctt aactgtcggt ggccttgtat caacgctgga ataaatccag
      781 gtaggtgggc cggcaggtac aacggctggt tgttcgttcc cttgctggtc ggtcatgtac
      841 tcacctttaa tttgatacat acaattctta cttaaattct ttcgggtatt taccagcgcg
      901 caaattagcc caccaatctg cacaatattt gaacctaggc tctacgctta aacacatggc
      961 acctacagat ccacgtattc tggcaatcca ccgagcatct tgcgattatg cagaaggctg
     1021 gagtggcgag gatggtatcc gcgcacacgc tcgggctaag gctgccgaaa ttggttgtgt
     1081 tgcagtaagt ccaggtgcag gggcagtatt gcgaactttt gcggctgcga ttgatgcaaa
     1141 aaatgttgtt gagattggca ctggtgctgg tgtttctgca ctttggttac ttgaaggcat
     1201 gaatcccgaa ggtgttttaa ccagcgttga taatgaagct gaacatcaat taatcgccaa
     1261 agatgcactt acccaagccg ggatttcacc aaatcgagtt cgtttaattt atggaaattc
     1321 ccaagaagtt ttggaccgtc tcactgactc ggcgtatgac attgtgctga tttctggccg
     1381 cccacttgaa ttagccgcac atgttgaacg tgcaatgcat ctgctcagcc caggcggtct
     1441 tttgataatt gatcgcgcac tgtggaatga caagcttgca gactcaagtc aacaagatgc
     1501 agagacttct gcagtccgcg ggatgattga atcgttagca accaatgaag atttaattgg
     1561 tgctttgcta cctgtcggtg gcggactgct cgtttcggta aaaaaatagc aaaataaaaa
     1621 gcgggtcaag gcccgctttt ttagtttaaa aaaattattt tttaggcatt gcagcaacag
     1681 tatctttaag tactgcgcct agatctttaa cttcttgtgc gttgagttca acgactagtc
     1741 ggccgccacc ttctagtgga atacgcaata cgtatccgcg accttctttt ggacactcca
     1801 ttggtccatc tccggtgcgt ggtttcattg cagccattta agtcctcttt cgacttgatt
     1861 ttgatatacc tattatctct taactgaata aaagtacgaa attgtaagcg ttaaaccttg
     1921 aatttattgg ggatgtaaat gtgatttatt gaattgactt attcggcaaa gtctgcaaca
     1981 tattttgcaa atttatctaa agacttttgt acgccaagta caaatagtgg ccgaactaat
     2041 ttgaatccaa agcggccaac gaaaccaaat ggcaggttta aatattcaat ccaaatgaac
     2101 ttgctgatgt ttttacttac ccgctcaact tgaaaactgc cggttccttt gacaattttg
     2161 ccggtatgta aaacgtcaca tcgatgaggc ggtttccagc gcgtgataat catggtgtcc
     2221 agaaatccaa tcggaccgat tccggtaaaa gcggaaattt cgccgtctac ctcagcaccg
     2281 gtgccgctga ctttggtgcc taacatccac tcactttgac ttggccagtc ggtgattcga
     2341 ttccagacaa gttcaactgg agcatgaact attacttcta ggcggatgta ctccactaga
     2401 acgccccaaa tggtggagga ttttcttcat atgtaccttg caaaattccc cattgattaa
     2461 ccaaattttt gcgctttgct tcagcgttaa ccaaaattcg aacaacttca tctaggtcat
     2521 cggtgatgtg taaagagtcg acatcttctt gcgaaatttt tccaccacgc agcatcgtgt
     2581 ttttgatcca gtcaacaaga ggtgtccaat atgctgaccc aactaaaacc accggaaatg
     2641 aggttacttt tttagtttgc accaaagtta gcgcctcaaa taattcatct aaagttccat
     2701 agccaccagg aaaaactacg aaaccttgtg agtacttaat gaacatagtt ttacgcgcaa
     2761 agaaatatct aaagtcgatt gccaactcaa cataatcatt catctgctgt tcaaagggca
     2821 gctctatacc aagtccaaca gaaagacctt ctccttctgt gcaaccttta tttgcggctt
     2881 ccataattcc tggtccaccg ccggtaatca cggcatactt tctttctgaa agttttttac
     2941 ctagttcata agcagattta tattctgaa
//
LOCUS       DBNHVN010000246         1383 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1830024, whole genome shotgun sequence.
ACCESSION   DBNHVN010000246 DBNHVN010000000
VERSION     DBNHVN010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1830024"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..942)
                     /gene="qcrC"
                     /locus_tag="AC16Y1_04640"
     CDS             complement(166..942)
                     /gene="qcrC"
                     /locus_tag="AC16Y1_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc1 complex diheme cytochrome c
                     subunit"
                     /protein_id="HIR5831782.1"
                     /translation="MAINRRHPAVMATVLGVALGTMGVAYAAVDSSTKPRTITVSQTQ
                     VEEGKALYIEGCSSCHGLDAQGGTDGPSLIGVGAASVDFQVSTGRMPMAAPGVQAPAK
                     KAVYNETEIAALAAYIASLGPGPAIPTAEMLDTSDAEIALGGELFRTNCAQCHSASGR
                     GGALSDGRIAPTLMNSDAKEIYEAMVSGPQAMPVFSDSTLPLEDKQAIIAYIQELQKN
                     ENPGGGSLGRLGPVTEGLFVWIFGLGALLAVAVWIGAKAK"
     gene            complement(982..>1383)
                     /gene="ctaE"
                     /locus_tag="AC16Y1_04645"
     CDS             complement(982..>1383)
                     /gene="ctaE"
                     /locus_tag="AC16Y1_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome oxidase subunit III"
                     /protein_id="HIR5831783.1"
                     /translation="FNTTILVLSSVTCQLGVFAAERGDVAKLRKWFTITLLMGTYFVA
                     GQVYEYVELVHEGLTLSSSAYGSAFYLTTGFHGLHVTGGLIAFIFVLLRTYATKHFTH
                     GQATSAIVVSYYWHFVDVVWIGLFAVIYLIQ"
BASE COUNT          375 a          341 c          301 g          366 t
ORIGIN      
        1 caaccccaaa catggttgca acttgaattt ctgcacgctt tgcagctttt ggatctatat
       61 cagcgcggcg aagctggtgt tcacccatcg ggaaaactgg aacaccgcta actacatcca
      121 aaccatcttt tgcgccatac ggttcaagcc cattgttctc gtgactcatt tagcttttgc
      181 tccaatccac acagcaacgg caagcagagc gccaagtccg aatatccaaa cgaataaacc
      241 ttcagtgacg ggacctagtc gacctagtga gccaccacct ggattttcat tcttttgcag
      301 ttcttgaatg taagcgatga tcgcttgctt atcttcaagt ggcaatgtgc tatccgagaa
      361 aactggcatt gcttgtggtc ccgaaaccat cgcttcgtaa atttctttgg catcagaatt
      421 cattagcgtc ggggcaatgc gaccatcgga caaagcaccg ccacgacctg aggcgctgtg
      481 gcactgcgca cagttagttc taaatagttc tccaccaagg gcgatttcgg catctgaggt
      541 atctaacatt tcagctgttg gaattgcagg tcctggtcca agtgaagcaa tgtaagcagc
      601 tagcgcagca atttctgttt cgttataaac agcttttttt gctggagctt gtactccggg
      661 tgccgccatt ggcattcgac cagtactcac ttggaagtca actgatgctg caccgacgcc
      721 gatcaatgag gggccgtcgg taccaccttg tgcatcaagt ccgtggcaag aagaacatcc
      781 ttctatatac agcgccttac cttcttcgac ctgggtctgt gaaacggtaa tagttcttgg
      841 ctttgtgctc gaatcaactg ctgcataagc aacacccatc gttccgagag caacaccgag
      901 aacagttgcc attactgctg gatgtctacg atttatcgcc actagctttc ctcgtttact
      961 tattttttaa attggttctt tttattggat tagataaata actgcgaaca aaccaatcca
     1021 gactacgtca acaaaatgcc agtagtaaga gacgactatg gctgatgttg cttgcccgtg
     1081 ggtgaaatgt tttgttgcat aagttctaag tagtacaaaa ataaatgcga tcaagccacc
     1141 tgtaacgtgc aacccgtgga agccagttgt taggtaaaaa gctgatccgt atgccgagga
     1201 tgaaagggtt agaccctcat gaacaagttc aacatattcg taaacctgtc cagcaacgaa
     1261 gtacgtaccc attaacaatg tgatggtgaa ccatttacgc aatttcgcaa catcgccgcg
     1321 ttctgcagca aacacaccca gctgacaagt cacagatgat aaaaccaaaa tagttgtgtt
     1381 gaa
//
LOCUS       DBNHVN010000247         3016 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1607118, whole genome shotgun sequence.
ACCESSION   DBNHVN010000247 DBNHVN010000000
VERSION     DBNHVN010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1607118"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1366)
                     /locus_tag="AC16Y1_04650"
     CDS             complement(<1..1366)
                     /locus_tag="AC16Y1_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR5831784.1"
                     /translation="MAKVTSAAKRLAFGRALASERMGDTLLPKRIALPVFASDAMSSV
                     AYASEEILLVLAMGGIALLTNSIYAALGVAVVMIVVVLSYRQNVNEYQSGGGDYEIAT
                     VNLGPKAGVTVASALLVDYVLLVAVSISAAVDNLSSSITALHDYKVALAVGLIIFLTM
                     INLRGVKESGTVLAFPTYAFLLGIFSMFVWGFFRISRGEQVLAESSSWQIIPEGEFTG
                     LVLAFLLLRAFTSGCAALTGIEAISNGVPAFRPPKGKNAATTLTLLGAIGVTMFIGIT
                     LLAQITQVRVTHDDALLIGKPENVEQKTVIAQIANAVFSDFPLIAGFIALVTALILVL
                     AANTGFNGFPLLGSILARDGFLPKQLQKRGDRLAHSNGIIALSAMAILLVIIFEAEVT
                     LLIPLYAVGVFTSFTLSQFGMIRHWSRRISAEIDVVAKREMVRKQSVNKVGFSLTGVV
                     LVIIL"
     gene            1458..2108
                     /locus_tag="AC16Y1_04655"
     CDS             1458..2108
                     /locus_tag="AC16Y1_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018227416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR5831785.1"
                     /translation="MHIVIMGCGRVGSLVAIELENSGHSVAIIDQEESSFKRLGKSFA
                     GQKILGIGFDRDRLTEAGISQADAFVAVSSGDNSNILAARVARETFGVKNVIARIYDP
                     KRAEVYERLGIPTVATVSWTADQILRHVNPIGATEDWHDPSGSVTLAQVAYHESWIGS
                     SISKIESKTNCNIAVITRYGKGILPDASTVLQDGDVLHVLATTDTLSNITKKLGMS"
     gene            2112..2765
                     /locus_tag="AC16Y1_04660"
     CDS             2112..2765
                     /locus_tag="AC16Y1_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR5831786.1"
                     /translation="MRIAIAGAGQVGRSIARELAANGHSILLLDRDSSVHKLAGENIE
                     CIQIDACEIESLSQAKLSECHVMVAATGDDKANLVISLLAKTEFGVSRVVARVNHPKN
                     EWMFDEAWGVDTAVSTPRILSALVEEAVSVGDLIRLMTLRQGQANLLEYTVEAASALA
                     GKRVGDVPWPSDTNLVAILREKKVITPSADDPLEVADELLFIAAEEQEPVLAKLLKG"
     gene            complement(2755..>3016)
                     /locus_tag="AC16Y1_04665"
     CDS             complement(2755..>3016)
                     /locus_tag="AC16Y1_04665"
                     /inference="COORDINATES: protein motif:HMM:NF022805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HIR5831787.1"
                     /translation="PILGYLIESIRGTRTDWQKNRETLRRYRAMTYLWLGVFSLRVLV
                     MTPLYLTNQIAALGFFRLALGLPLFALAGYATFALSKRQVNP"
BASE COUNT          890 a          653 c          674 g          799 t
ORIGIN      
        1 ttaaaatgat tactaaaact acgccagtaa gcgaaaagcc aactttattg acactttgct
       61 tgcgtaccat ttctcgtttt gctactacat cgatttccgc actaattctt cttgaccagt
      121 gacgaatcat tccaaattga ctcaaagtaa atgaggtgaa aactccaact gcataaagtg
      181 gaattaacag ggtaacttcc gcttcaaaaa taattaccag taaaattgcc attgctgaaa
      241 gtgcaataat tccattactg tgtgcgagac gatcgccgcg tttttgtaat tgttttggca
      301 ggaacccatc tcttgccaaa attgagccga gtaacggaaa accattaaag ccagtatttg
      361 ccgctaaaac caaaattaag gcggttacta gcgcaatgaa accagcaatc aacggaaagt
      421 cactgaatac tgcattcgct atttgagcaa ttacggtttt ttgttcaacg ttttctggct
      481 ttccaatcag taacgcatca tcgtgtgtga ctctgacttg tgtgatctgg gcaagcaaag
      541 ttataccaat aaacatcgtc acaccaattg ctcccaggag cgtcaaggta gtggcagcgt
      601 ttttaccctt tggcggtcga aatgcgggta caccattact tattgcttcg atgccggtta
      661 gtgcagcaca tcctgaggta aaggctctta acaataaaaa cgcaagaaca agtccagtga
      721 attcaccttc tggaattatc tgccaactcg aactctcggc gagtacttgt tcacctcgag
      781 aaattcggaa aaagccccaa acaaacattg aaaaaatgcc aagtaagaat gcgtaagtcg
      841 gaaacgcaag aaccgtcccc gattctttaa cacctcttaa gttgatcatt gttaaaaaga
      901 taattaaacc tacggctaaa gccactttgt aatcgtgcag agctgtaata cttgaactca
      961 aattatcgac tgctgcagaa atagatacgg caactagaag aacataatca actaacaagg
     1021 cacttgcgac agtaactcct gccttaggtc ccaaatttac ggtcgcaatt tcataatctc
     1081 cgccaccgct ttgatactcg ttaacgtttt ggcgatacga caaaacaaca acgatcatta
     1141 ctactgccac acctagggcg gcgtaaattg aattggtcag aagtgcaatg ccacccatgg
     1201 ccaggactaa caaaatttct tcagatgcgt aggcgacgct ggacatcgca tctgaggcaa
     1261 aaactggaag ggctatgcgt ttaggtaaaa gagtgtcgcc cattcgctca ctagcaagtg
     1321 cgcgaccaaa agcaaggcgc tttgccgccg aggtaacttt tgccacaagg agagcctacg
     1381 cctgttccgg taaggctctt ttccaggtcg ccttaggtct aatgtaaata aatcaaatag
     1441 acgtgtagtt ttagttcgtg cacatagtga ttatgggttg cggtcgagtt ggatcgctgg
     1501 ttgcaattga acttgaaaat tcaggtcatt cggttgcaat tattgatcaa gaggaatctt
     1561 catttaagcg actaggcaag agttttgctg gtcaaaaaat tttaggaatt ggttttgacc
     1621 gcgatcggtt gaccgaagct ggtatcagtc aggcagatgc ctttgtcgca gtatcgtctg
     1681 gagataactc aaacattctc gccgctaggg tagctcgtga aacttttggt gtaaagaatg
     1741 ttatcgcccg aatttacgat cctaaacgtg ccgaggttta tgaacgcttg ggtattccaa
     1801 cggtagcaac tgtttcttgg actgcagatc aaatcctgcg ccatgtgaat ccgataggtg
     1861 caactgaaga ttggcatgat ccaagtggca gcgtcaccct tgctcaagtt gcttatcatg
     1921 aatcttggat tggaagttca atctcaaaaa ttgagagcaa aacaaattgc aacattgctg
     1981 ttataacccg ctacggaaaa ggaatactgc ccgacgcaag tacagtcctt caagatggcg
     2041 atgtgctaca tgtacttgca acaactgata ctttgtcgaa cattactaaa aaacttggaa
     2101 tgagctaatc gatgcgaatt gcaatagctg gagcggggca agtaggtcga tctatcgcgc
     2161 gcgaattggc agctaatgga cacagcattt tgcttctcga tcgagattca agtgtgcata
     2221 agttagctgg cgaaaacatt gaatgtattc aaattgacgc ttgcgagatt gaatctctca
     2281 gtcaagccaa actgtcagag tgtcatgtaa tggtagctgc taccggcgac gataaagcca
     2341 atctcgtgat cagcctgctg gcgaaaacag agtttggagt ttctagagtt gtggctcgcg
     2401 ttaaccatcc gaaaaatgag tggatgtttg atgaggcctg gggtgttgat actgctgtgt
     2461 ctactccacg aattctttct gcattagtag aagaagcagt ttcagttggc gacttaatcc
     2521 gtctcatgac tctgcggcaa ggtcaagcta acttacttga atacactgtg gaagctgcca
     2581 gcgcgctagc aggtaaacga gtaggcgatg tgccgtggcc aagtgacacc aatttagttg
     2641 cgattttgcg tgagaaaaaa gtaatcacac cttccgcaga tgatccacta gaagttgcag
     2701 atgaattgct atttatcgca gccgaagaac aagaaccagt tctggctaaa ctacttaagg
     2761 gttaacttgc cgcttactca aagcaaatgt tgcgtacccc gctagtgcaa aaagtggtaa
     2821 gccaagtgcg agtctaaaaa atccaagtgc cgcaatttga ttggttaggt atagcggagt
     2881 cataaccaaa acccgcaagc tgaatacacc caaccataaa taggtcattg ctctgtatct
     2941 acgcagagtt tcgcgatttt tttgccaatc cgttctagtg ccacggattg attcgattaa
     3001 gtagccaagt attggt
//
LOCUS       DBNHVN010000248         2267 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1088433, whole genome shotgun sequence.
ACCESSION   DBNHVN010000248 DBNHVN010000000
VERSION     DBNHVN010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1088433"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="AC16Y1_04670"
     CDS             <1..267
                     /locus_tag="AC16Y1_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831788.1"
                     /translation="YFFVFLGPYVFSNGALGFGDVKYAISCGFVIGVVDAKVWLDVIW
                     LMFILAGVFGLIMIMAKKVGLMSAIAFAPFMTASVFVFSLNSLH"
     gene            292..1470
                     /gene="aroC"
                     /locus_tag="AC16Y1_04675"
     CDS             292..1470
                     /gene="aroC"
                     /locus_tag="AC16Y1_04675"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041834988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR5831789.1"
                     /translation="MLRWLTAGESHGPALTAILEGLPAGVEVSTADLNKGLARRRLGF
                     GRGARQKFEQDELAITGGVRHGLTQGGPIAINIKNTEWSKWELVMSADPVNVADLADL
                     ARNAPLTRPRPGHADLAGMQKYSLDDARPILERASARETAARVALGVLAKKFLEQVAQ
                     IHVYSHVVSIGTITTEETQAPTPDDVERIDEDPVRCFSVSASKAMQEEISKAHSDGDT
                     LGGVVEVLATNVPVGLGSHVHWDRRLDSRLAAALMGIQAIKGVEVGDGFETARRRGSV
                     AHDEIYTSDGKLVRHTSRSGGTEGGMSTGEILRVRAAMKPISTVPRALATVDVATGQE
                     ATAINQRSDACAVPAAGVIAEAMVALVLADAFLEKFGGDHLTETRRNYQSFKENLKIQ
                     "
     gene            1472..1987
                     /locus_tag="AC16Y1_04680"
     CDS             1472..1987
                     /locus_tag="AC16Y1_04680"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR5831790.1"
                     /translation="MAIVLIGVPGAGKTTVGSRLAKHLNFDFVDSDQQIEKLTGKSIS
                     DIFVQDGEEVFRKIEAEVIAAALAQTQVVVSVGGGALTNENTRNLVKSHTAIWLQAGL
                     SQAVDRIGMNKNRPLLLGNVRGQLADLMTAREPFYIECAKLVVDTNELTPDEVASKIE
                     QLLGGNFNADN"
     gene            1974..>2267
                     /locus_tag="AC16Y1_04685"
     CDS             1974..>2267
                     /locus_tag="AC16Y1_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR5831791.1"
                     /translation="MPTIEVNAEHTYQVVIGRNLLDQIADSVAGADRVAVLHPRALRE
                     TGELIRSSLNDVTSIAIEIPEAEEAKTFDVLKFCWMALGQSGFTRNDVVIAVGG"
BASE COUNT          612 a          452 c          597 g          606 t
ORIGIN      
        1 tacttttttg tatttcttgg gccatacgta tttagtaatg gcgcccttgg ttttggtgat
       61 gtcaaatacg caatttcgtg tggctttgtt attggcgtgg tagatgcaaa ggtgtggctg
      121 gatgtgatat ggctgatgtt catccttgct ggagtttttg gtttaatcat gattatggcc
      181 aaaaaggttg gactaatgag cgccattgca tttgcaccat tcatgaccgc atctgtattt
      241 gtatttagtc tcaattcgct ccactaagga aattagtgaa agaataggaa aatgttgcgt
      301 tggttaactg ccggagaatc tcacggccct gcgctgactg caattcttga aggtcttccg
      361 gcaggggttg aagtgtctac tgccgattta aataaaggac tggctcgtcg ccggctgggt
      421 tttggtcgag gagcaagaca aaagtttgag caagatgaat tagcgatcac cggtggggta
      481 agacatgggc ttactcaggg tggcccgatt gcaataaaca ttaaaaatac tgaatggtcc
      541 aagtgggaac tagttatgtc cgcggatcca gtaaatgttg cggacctagc agatttagct
      601 cgcaatgctc cgctaaccag gcctcggccc ggacatgctg atttggccgg aatgcagaaa
      661 tactctttag atgacgcacg tccgattctc gaacgggcaa gtgcacgcga aactgcagcc
      721 cgtgttgctt tgggagtact agcaaaaaaa ttcctggaac aagttgcgca gatacatgtt
      781 tacagtcacg tggttagtat cggcacgatc acaacagaag aaacacaagc tccaacacct
      841 gatgacgtcg agagaatcga tgaagatcca gttcggtgct tctctgtctc ggctagtaaa
      901 gcaatgcaag aagaaatctc taaagcacat tctgatggtg acactcttgg tggagtcgtt
      961 gaagttcttg ctaccaatgt tccagtcgga ttaggctcgc atgttcactg ggatcgtcgt
     1021 ttagacagtc gactagcagc agcgctgatg gggatacaag cgattaaagg tgtcgaagtt
     1081 ggagatggtt ttgaaacagc tagaagacgt ggatcagttg cgcacgacga aatttacacc
     1141 tcggacggca aattagtccg ccatacgtcg cgtagtggtg gtacagaagg tgggatgtcg
     1201 acgggcgaaa tactcagagt tcgagcagct atgaaaccta ttagtactgt tccacgtgcg
     1261 cttgccacag tagacgtcgc gactggacaa gaggcaaccg caattaatca acgttcagat
     1321 gcatgtgctg tgccggctgc tggggtaatc gccgaagcga tggttgcact tgttctggca
     1381 gatgctttcc tcgagaaatt cggcggagat cacttaactg aaactagaag gaactaccaa
     1441 agtttcaaag aaaatttgaa gattcagtaa catggccatt gtcttaattg gtgtcccagg
     1501 tgcaggaaaa accactgtcg gctcccgctt ggctaagcat ttaaatttcg attttgtaga
     1561 tagcgatcag caaatagaaa agttaaccgg aaaatcgatt tctgatattt ttgtgcaaga
     1621 tggggaagaa gtttttcgaa aaattgaggc cgaggttatt gcagcagctc ttgcacagac
     1681 tcaagtcgtt gtatcggttg gtggtggcgc tttaacaaat gagaataccc gtaatttagt
     1741 taagagccac acagcaattt ggctacaagc aggattatcg caagcggttg atcgaatcgg
     1801 aatgaataaa aatcgaccat tattgcttgg caatgttcgt gggcaacttg cagatttgat
     1861 gacggcccgc gaaccgtttt acatcgaatg cgccaaacta gttgttgata caaatgaact
     1921 aacaccagat gaagttgctt cgaaaattga gcagctattg gggggaaact ttaatgccga
     1981 caattgaagt taacgccgaa catacttatc aggtcgtaat tggccgtaat ttacttgatc
     2041 agattgctga ttcagttgca ggtgctgatc gcgtagcagt gctgcatccg cgagccctgc
     2101 gagaaaccgg cgagctgatt cgaagctcat tgaatgacgt cacctcaatt gctatcgaga
     2161 ttcctgaagc agaagaggca aagacttttg atgttttgaa attctgttgg atggcattgg
     2221 gtcagtccgg atttacccgc aatgatgttg taatcgctgt cggtggt
//
LOCUS       DBNHVN010000249         1752 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1607167, whole genome shotgun sequence.
ACCESSION   DBNHVN010000249 DBNHVN010000000
VERSION     DBNHVN010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1607167"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="AC16Y1_04690"
     CDS             complement(<1..227)
                     /locus_tag="AC16Y1_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018761336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--1-D-myo-inosityl
                     2-amino-2-deoxy-alpha-D-glucopyranoside ligase"
                     /protein_id="HIR5831792.1"
                     /translation="MQPWNSVTVPKTTFPVPAPKIYDSKTQKLSQLQISADTSIYVCG
                     ITPYDATHLGHAATYVAFDTLIRVWTAAGST"
     gene            complement(233..1246)
                     /gene="gnd"
                     /locus_tag="AC16Y1_04695"
     CDS             complement(233..1246)
                     /gene="gnd"
                     /locus_tag="AC16Y1_04695"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013828910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HIR5831793.1"
                     /translation="MIGLGRMGANIVKRLAEHEISSVVYDTNSQSRNDLAAFNSALIS
                     AAQSIPELISKLTSPRTIWVMVPAGDITESVISELIENLAADDIIIDGGNSYYRDDLK
                     HAKSAQAKGIHFLDVGTSGGVWGLERGYCLMIGGNEKAVGQLTHIWNALAPGIDSAPR
                     TPGKSGVVTPAEQGWLHCGPSGSGHFVKMIHNGIEYGLMAAYAEGLNILQNANEGSRS
                     RINDAETAPLSNPEFYQYDFDIASITELWRRGSVVSSWLLDLTAQALNQSPELSEYSG
                     QVSDSGEGRWTAIAAIEEGIPAPVLTAAVFSRFESRSRGMFANKVLSAMRKQFGGHQE
                     KQG"
     gene            1351..>1752
                     /gene="pgi"
                     /locus_tag="AC16Y1_04700"
     CDS             1351..>1752
                     /gene="pgi"
                     /locus_tag="AC16Y1_04700"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012562.7"
                     /note="functions in sugar metabolism in glycolysis and the
                     Embden-Meyerhof pathways (EMP) and in gluconeogenesis;
                     catalyzes reversible isomerization of glucose-6-phosphate
                     to fructose-6-phosphate; member of PGI family; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIR5831794.1"
                     /translation="MLGSPVTSAPEWASLVALAKAFNNKHLRELFAANPERLSSLVIS
                     APNLTVDISRQRVDSDVLDRLLSLARSRQLLEAITAMFSGQKINNTEDRAVLHTALRL
                     PKTKSLEIDGQDVVPAIHDVLERMSSFANQIR"
BASE COUNT          491 a          409 c          364 g          488 t
ORIGIN      
        1 atggtgcttc ccgcggctgt ccagactcta atgagcgtat cgaaagcaac gtaggtagca
       61 gcatgtccta aatgcgtcgc atcgtaagga gtaattccac atacataaat agaagtgtca
      121 gcactaattt gtagttggct taatttttgc gtctttgagt cataaatttt tggagccggg
      181 acagggaaag tggttttagg gaccgttact gagttccaag gctgcatcag aactagcctt
      241 gcttttcttg atgtccacca aattgcttgc gcatagcaga cagcactttg tttgcaaaca
      301 ttccacggga ccttgactca aatcgcgaga acactgctgc tgttagaacc ggtgctggga
      361 taccctcttc aattgctgcg atggcagtcc accgaccttc accagaatca gatacttgac
      421 ctgaatattc agaaagttct ggcgactgat ttagtgcttg ggcggttaaa tcaagtagcc
      481 atgaagacac aacgctacct ctacgccaaa gttctgttat cgaggcaatg tcaaaatcat
      541 attgatagaa ctctggatta ctaagtgggg cagtctcggc atcattgatt ctggatctac
      601 taccttcatt tgcattttgc aaaatgttaa gaccttcggc gtaagcagcc atcaagccgt
      661 actcaattcc gttgtgaatc atctttacaa agtgaccaga ccctgatgga ccacaatgta
      721 accaaccttg ttcagccggt gtcacaactc cagatttgcc aggcgttctt ggcgcactat
      781 caatccctgg agctaacgca ttccaaatgt gggttaattg cccaacggct ttttcgtttc
      841 caccgatcat taagcaataa ccacgctcga gaccccagac accaccactg gtgcctacat
      901 caagaaaatg aatacctttt gcttgggcgg actttgcatg ctttagatca tctcggtaat
      961 aactattacc accatcgata atgatgtcgt ccgctgccaa gttttcgatc aactcagaaa
     1021 tcacggattc agttatgtca cctgcgggca ccataaccca aatagtgcgc ggggaagtca
     1081 atttactaat taactcagga atagattgtg cggcagatat cagtgctgaa ttgaaagctg
     1141 ctaaatcatt acgagattgt gaattagtgt cataaacgac gctactaatt tcatgttcag
     1201 caagtcgctt tacaatgttt gcgcccattc ggcccaaacc aatcattccg aaatgatttg
     1261 cttgatttgc cacgccaaac tccaatactt catgggcgca cgagaaaaat taacctagtc
     1321 ttagattacc ttgatttagg ggagtattaa atgcttggtt cgccagtaac ttctgcgcca
     1381 gagtgggcga gtttagttgc gcttgctaaa gcatttaaca ataaacactt acgggaactt
     1441 tttgcagcca atcccgaaag actttcaagt ctggtcataa gcgcaccaaa tttaacagtc
     1501 gatatttctc gacaacgtgt tgattcagat gtgctagatc gcctgctttc cttagcaagg
     1561 tcgcgtcaat tacttgaagc catcaccgct atgttttccg gacaaaaaat taataacacc
     1621 gaggatagag cagtcttaca taccgcatta cggttaccta aaactaaatc tttagaaatt
     1681 gatggccaag acgtagttcc ggccattcat gatgttttag aacgaatgag ttcatttgcc
     1741 aatcaaatcc gt
//
LOCUS       DBNHVN010000250         2081 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_195200, whole genome shotgun sequence.
ACCESSION   DBNHVN010000250 DBNHVN010000000
VERSION     DBNHVN010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_195200"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="AC16Y1_04705"
     CDS             complement(<1..668)
                     /locus_tag="AC16Y1_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR5831795.1"
                     /translation="MTDQEVAQHLAEQIGQVLVEVQSTNLNKLSEEQLGAAGDLAGHE
                     CAIRELSKLRPLDHILSEEGEDNVGRLSANRVWIIDPLDGTSHYSRGETEFAVHVALW
                     QRDSSRPFQLEAFAVGVPRKDLVIGINEVKPTQTHDGPIRILVSATRPPEEIAKIKQG
                     LLKAFPQKGEPVLIPMGSVGAKLASIIQGQADLYLNTGGFYEWDIAAPAAVAKAHGLM
                     VCDAA"
     gene            complement(665..1243)
                     /gene="pth"
                     /locus_tag="AC16Y1_04710"
     CDS             complement(665..1243)
                     /gene="pth"
                     /locus_tag="AC16Y1_04710"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR5831796.1"
                     /translation="MSTTDAWLVVGLGNPGPTYERTRHNIGAVVIDELAARFNSSLKR
                     HKRALAEVAEIKIDEHKVVIAKPLSYMNESGGPVKALATFYKVPLEQIIVLHDELDIA
                     LGTIRVKLGGGDNGHNGLKSIRGAFNNGDWYRVRLGIGRPPGNQDPSDFVLKPFSTTE
                     ATEVSILRENGADATQKLITDGLLETQSTFNS"
     gene            complement(1247..1711)
                     /locus_tag="AC16Y1_04715"
     CDS             complement(1247..1711)
                     /locus_tag="AC16Y1_04715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HIR5831797.1"
                     /translation="MTKLKAEARNDFGKGAARRLRRDGKVPAVVYGHGTKPVHVAIDA
                     HELQLVLRKHQSSIDVSIDGKTETVAPRDIQIDPVTRKLEHLDLVIVTAAEAAKLARD
                     AAEASAKAEAAAVAAAENAAAKAAVRAERTEAEEAAASAASASTEPAGDSAE"
     gene            complement(1883..>2081)
                     /locus_tag="AC16Y1_04720"
     CDS             complement(1883..>2081)
                     /locus_tag="AC16Y1_04720"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006548850.1"
                     /note="catalyzes the formation of 5-phospho-alpha-D-ribose
                     1-phosphate from D-ribose 5-phosphate and ATP; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR5831798.1"
                     /translation="LSEPAAARLQESGIKEVVVTDTLPISADKHFERLTVLPIAPLIA
                     RAIREVFDEGSVTSLFDHVGV"
BASE COUNT          519 a          502 c          441 g          619 t
ORIGIN      
        1 gctgcatcac aaaccattaa gccatgagct ttggcaactg ctgccggagc tgcaatatcc
       61 cactcataaa atccgccagt atttaaatac agatctgctt gcccttgaat aatgctggct
      121 aacttcgccc cgacagatcc catcggaatt aaaactggtt cacctttttg cggaaatgcc
      181 ttaagtaatc cttgtttaat ttttgcgatc tcttctggtg gcctggttgc tgaaactaaa
      241 attctgatcg gtccatcatg tgtttgtgtt ggcttaactt cattaattcc gattactaaa
      301 tcttttctgg gcacgccaac tgcaaatgct tctaattgaa atgggcgact actatcccgt
      361 tgccaaaggg cgacgtgtac cgcaaactcc gtttcgcccc gggaatagtg cgatgtgcca
      421 tcaagtggat caataatcca aactcgattt gcggataggc gtcctacgtt atcttcgcct
      481 tcttcactca aaatgtgatc aagtggcctt aacttactaa gttcgcgaat cgcacattca
      541 tggccagcta aatcgccagc tgcgccaagt tgttcttcac ttaacttatt cagatttgtc
      601 gattgaactt cgactaaaac ttggccaatt tgttcagcta aatgctgcgc aacttcttga
      661 tcggtcatga attaaatgtg ctttgagttt cgagtaatcc atcagtaatt aatttttgcg
      721 tcgcatctgc tccgttttca cgaagtatcg aaacttcagt tgcttcggtt gtactaaaag
      781 gttttaacac aaaatcactt ggatcttgat tacctggtgg ccggccgata cccaatcgaa
      841 ctcgatacca gtcaccattg ttaaatgcgc cgcgaattga tttaagccca ttgtgcccgt
      901 tatctccgcc accaagtttg actcgaatcg tgccaagtgc aatgtctaat tcatcgtgca
      961 aaacaatgat ttgctcaagt ggaactttat aaaaagtagc cagcgcttta actggtccgc
     1021 cagattcatt catgtaagaa agtggtttag caatcacaac tttatgttcg tcgattttta
     1081 tttctgctac ttcagctaat gctcgtttat gacgtttcaa cgaactgtta aaacgcgctg
     1141 ctaattcatc gatcactacc gcaccgatgt tatgccgcgt gcgctcgtaa gtggggccag
     1201 gattacctaa ccccactacc agccatgcgt cagtagttga cactaattac tcagcagaat
     1261 cgccagcagg ttcagtgctt gcactagcag cacttgccgc agcttcttca gcttcggtgc
     1321 gttcagcgcg aactgcagct ttagccgctg cattctcagc agcagcaacc gctgcggctt
     1381 cagctttcgc tgatgcttca gctgcatcgc gagctaactt agctgcttct gctgcagtca
     1441 caattactag atctaagtgt tctagtttgc gagttactgg atcaatttga atgtcgcgtg
     1501 gtgcaacggt ttcagttttt ccatcgattg aaacatcaat tgaagattgg tgtttacgaa
     1561 gtactagttg tagttcgtgt gcatcaattg caacgtgaac tggctttgta ccgtggccat
     1621 aaaccactgc aggaactttg ccatcgcgac gtaggcgacg agcagcacct ttaccgaaat
     1681 cattacgtgc ttcggctttt agttttgtca ttattctcct cggggtcgtg cggaatgctc
     1741 cacagcagac cttcgtcgat tacggctaag tttttgtagt ttctacaatc acattgccct
     1801 cgccgaggag cctttatagg ttatcgactg agcgccggat aggcaaaaag ataatcgaat
     1861 aacggtttta gttggttatt tactaaacgc caacgtgatc aaaaaggctt gttactgaac
     1921 cttcatcaaa tacttcgcga atagcccgcg caataagtgg tgcgataggt aacacagtta
     1981 agcgctcgaa atgtttgtcc gcagaaattg gcagggtgtc cgtaactaca acttctttaa
     2041 tacctgattc ttgtagtcgc gcagctgctg gttcggaaag c
//
LOCUS       DBNHVN010000251        14772 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_49388, whole genome shotgun sequence.
ACCESSION   DBNHVN010000251 DBNHVN010000000
VERSION     DBNHVN010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 14772)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14772)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14772
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_49388"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="AC16Y1_04725"
     CDS             <1..484
                     /locus_tag="AC16Y1_04725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5831799.1"
                     /translation="KLNLDGARPWSWILASLSLGMALGVVVAMRVRPKHPLVLGLLAQ
                     VALVSFMIVLGVSTNVWLIMLFAFFCGIGMDLFMILWQTSLQTHIPRESLSRVASYDA
                     FGSLALAPLGLVLAGPAAERFGVETAIVTLGVICAITLAAMISVPGVRKLEAKEPETV
                     "
     gene            629..2056
                     /locus_tag="AC16Y1_04730"
     CDS             629..2056
                     /locus_tag="AC16Y1_04730"
                     /EC_number="1.13.12.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HIR5831800.1"
                     /translation="MNSLGLPPIIQGGMGAAVSSWQLAHTVASLGQLGVVSGTALETV
                     VARRLQDGDEGGHMRRALAAFPYQDVAQHVLDTFYLPEGRKGEPYRPMRRISIKAHKE
                     HDQLSVAANFVEVWLAKQAGAGKVGINFLEKLQTATPAALYGAMLAGVDAILMGAGIP
                     REIPQLIRDFADGKPGHLSIDSERPSGVDAPILEFDPLESFGAAPKLNRPAFLAIVTA
                     EVLAAYLARNEITRPDGFVVEHYHAGGHNAPPRRMQDTETGYGPLDEPNMQKIADVGL
                     PFWLAGGRATPESVAEAFALGASGVQVGSLFALSNESGLLPKYREQMLDAARKGNLRV
                     RTDHRASPTGFPFKVVELPGTVGEESVYQARPRLCDLGYLRSSHIEDSGKVTYRCSAE
                     PESPFLKKGGEEDDLRARICLCNGLMAAIGLGQERADGYKEAPLLTLGSTTGDVEAML
                     KEFPNGWSAADVVNRLLASVPATAK"
     gene            complement(2058..2552)
                     /locus_tag="AC16Y1_04735"
     CDS             complement(2058..2552)
                     /locus_tag="AC16Y1_04735"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HIR5831801.1"
                     /translation="MALEFDVLVEIPGGSRNKYEVDHETGRLRLDRMLFTSTRYPNDY
                     GYIENTLGQDGDPLDALVLIDEPTFPGVLIRCRAIGMFHMRDEAGGDDKIMCVPAKDI
                     RKNHLQDIQDIPEFDRLEIQHFFETYKDLEPGKSVEGAHFEDRASAEAEILASFERLK
                     KQSH"
     gene            2634..3581
                     /gene="tilS"
                     /locus_tag="AC16Y1_04740"
     CDS             2634..3581
                     /gene="tilS"
                     /locus_tag="AC16Y1_04740"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR5831802.1"
                     /translation="MINLELRSALLTNLADLSPGDAIAVGVSGGADSVALLKAAVHVG
                     KELTLNVHAVVIDHQLQPNSNEVANKTAQLATELGATELEVVPVQVATGPGSGGVEAA
                     ARNARRRAFETYVAKNKIAAVLLGHTLEDQAETVLLGLARGSGAKSLAGMQSIDGIYR
                     RPFLMLSRELVRSTVSDLPTYEDPHNSDLSFARVRVRNNVLPVLEKELGPGITQALAR
                     TAEMLREDTQALDSYAQMILAAEDVGISTLEVLPAAVRTRVIRKLAINSGVLINDLTR
                     DHVLNIDQLITNWKGQGPLNLPGQVNVKRESGKLIFYKP"
     gene            3594..4154
                     /gene="hpt"
                     /locus_tag="AC16Y1_04745"
     CDS             3594..4154
                     /gene="hpt"
                     /locus_tag="AC16Y1_04745"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR5831803.1"
                     /translation="MDAASIGDELTSVVLTEEQLRTRIKEIAAQIDNDYANLDKPLLL
                     VGVLKGAVMVMADLARAISIPVQFDFMAVSSYGSGTKSSGVVRIIKDLDIDLTGRHVL
                     IIEDILDSGLTLSWLIKNLKSRGPVSIEVMTMMRKPDAIKVEVNARYIGFDIPNEFVV
                     GFGLDYAERYRNLREVGVLAPHVYGG"
     gene            4277..6436
                     /gene="katG"
                     /locus_tag="AC16Y1_04750"
     CDS             4277..6436
                     /gene="katG"
                     /locus_tag="AC16Y1_04750"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006459806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HIR5831804.1"
                     /translation="MDNSAKCPVEHGGQTKAGYSPMDWWPNALNLEILHQHDTKTNPL
                     GEDFDYRKEVKSLDFAALKKDLHELMTSSQDWWPADWGHYGGLMIRLSWHAAGTYRVS
                     DGRGGAGTGSNRFAPLNSWPDNTNLDKARRLLWPIKKKYGNKISWADLLVLAGTIAYE
                     SMGLKTYGFAFGRPDIWDLEKDVYWGSEKEWLAPSDERYENVDDASTMENPLAAVQMG
                     LIYVNPEGVNGKPDPLKTAQHVRETFARMAMNDEETVALTAGGHTVGKAHGNGRAENL
                     GPEPEGADISEQGLGWNNHKTRGIGNSTVTSGIEGAWTTNPTKWDNGYFDLLFKYEWE
                     LKKSPAGANQWEPKNISEEDKPVDSEDPTKRYNPIMTDADMAMIKDPIYREISERFRN
                     DQDYFSDTFARAWFKLTHRDMGPKVRYIGPDVPAENLIWQDPIPAGNKNYDVAAVRKK
                     IEASGLSVSQMVSTAWDSARTFRGSDMRGGANGARIRLAPQKDWAGNEPERLQKVLAT
                     LEPIAKETGASLADVIVLAGNIGVEKAAKAAGFDVNVPFAPGRGDATQEQTDVESFAV
                     LEPLADGFRNWLKKDYVVSPEELLLDRAQLMGLTGHEMTALLGGMRVIGTNHGGAKHG
                     VFTENVGALTNDFFVNLTDMIYVWEPAGNNLYNIRNRKTGEVKYTATRADLVFGSNSI
                     LRAYAEVYAQDDNKKKFVNDFVAAWVKVMNADRFDLE"
     gene            complement(6495..6827)
                     /locus_tag="AC16Y1_04755"
     CDS             complement(6495..6827)
                     /locus_tag="AC16Y1_04755"
                     /inference="COORDINATES: protein motif:HMM:NF017880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HIR5831805.1"
                     /translation="MTSSTILHLTTKPVWEAAVEKGFYTQSTLDQTLEEVGFIHASFP
                     DQLAETARLHYSASTDELVLLEMKIGTLISAGLEVKVEESRNGQLFPHIYAPIPIGLI
                     AKVTPYQI"
     gene            complement(6997..7081)
                     /locus_tag="AC16Y1_04760"
     tRNA            complement(6997..7081)
                     /locus_tag="AC16Y1_04760"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7045..7047),aa:Ser,seq:gga)
     gene            7149..7931
                     /locus_tag="AC16Y1_04765"
     CDS             7149..7931
                     /locus_tag="AC16Y1_04765"
                     /inference="COORDINATES: protein motif:HMM:NF015747.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR5831806.1"
                     /translation="MRIGVIGVGTISTNVIEAMQKLNTSHTFYLSPRSNQRAKDLASK
                     YKNCIQVNSNQGVVDNSDIVIVSVLPKQVDEVLTELTFRPDQIITSFIAGRPPSEISK
                     LTVPATKVAQLIPLPPIELHKGPLLICPGIPELIETFSGLGDVIPFDDEQNIKVFSSA
                     SAVMSTYFRLVLEIVDWITAQGIDRTLATKYMTSLFEGLGTESNHIAPDLLTSMVREH
                     ETPGGLNESVRTNLEKSEWYADVRAQLDYLIKVFIPALKNQK"
     gene            7962..8057
                     /gene="ffs"
                     /locus_tag="AC16Y1_04770"
     ncRNA           7962..8057
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="AC16Y1_04770"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            8094..9848
                     /locus_tag="AC16Y1_04775"
     CDS             8094..9848
                     /locus_tag="AC16Y1_04775"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019852443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma and tau"
                     /protein_id="HIR5831807.1"
                     /translation="MSLALYRKYRPGQFADVIGQEHVTVPLSRALDNDRLHHAYLFTG
                     PRGCGKTSSARIMARSINCANGPTSTPCGVCQSCIDLAPNGPGSIDVIEIDAATYRGI
                     DDAKELRERAVYAPANSRYKVYIIDEAHQLTRDAFNVLLKLVEEPPPHLRFIFATTEP
                     DKIIPTLRSRTHHYPFRLVSAKNLAEHLGIICQREGVKADPASLALVARAAAGSVRDA
                     QSILGQVIAGAGSEGITYSETVAQLGFTDERLLGDVVVAIAKGDGAKLFTLVDQVVTT
                     GHEPRRFVADLLERLRDLLIVTQIPDSANSGLFDLPAEQVSDLVETAKLFTYGQLVRV
                     ADLVNSGMSELRGAASPRLQLELLVARLVTPEVAQDLAQRVAKLEQGGAPSYQRPVSE
                     PAAAKTQAVEPKVTPTAPKAAPVARTQDAPPPMKPSQVAAAAHVKATEEKPVEAKPQG
                     EITFTLLRANWVGILETLKTQSRVAWLVLQDTVPVSISDDRIVTVAFSSAGAMNNSIK
                     SKHDEKLRDAIKKVLNADVRIEGTVDPKHAKSPAAKKEVADAGEASPDDEAVTKRSAL
                     DMVTNMLGAEIISESPRQ"
     gene            9857..10456
                     /gene="recR"
                     /locus_tag="AC16Y1_04780"
     CDS             9857..10456
                     /gene="recR"
                     /locus_tag="AC16Y1_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014912795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR5831808.1"
                     /translation="MYEGIVQDLIDEFAKLPGVGPKGATRIAFHLLNSDHHDVTRLAE
                     VLREVKEKVRFCKECYNVAQNEHCRICADNRRDQTSICVVEEPKDVLAVERTREFRGK
                     YHVLGGAISPIDGIGPDDLRIKELMSRLASNSVSEVILATDPNLEGEATATYLARLLS
                     PLGLKVTRLASGLPVGGDLEYADEITLGRAFEGRRQVSP"
     gene            10523..11821
                     /locus_tag="AC16Y1_04785"
     CDS             10523..11821
                     /locus_tag="AC16Y1_04785"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR5831809.1"
                     /translation="MSQIVMKFGGSSVADAASVLRVAKRIVDNKNAGNEVVVIVSAMG
                     DTTDELLDLAKEVSPNPPGRELDMLLTAGERISMAVLAMAISDLGVEARSYTGSQAGL
                     ITDSTHGKARIVDVTPGRIKEALADGAIPIIAGFQGVAQDTKDITTLGRGGSDLTAVA
                     IAAALNADVCEIYTDVDGIFSADPRIAPKARQVPRITYDEMMELAAAGAKVLHLRCVE
                     YAKRFDVPIHVRSSFSQREGTFVIADLSDQSKIQKLSQQGAVMEQPIIAGVAHDLNDA
                     KITVVAVPDKPGTAAAIFQAVADSGINMDMIVQNVSASGATATDITFTVPRVDAAKAE
                     LALRKVQSEIGFKDIDTDENIAKISLVGAGMRSHPGVSATFFKAIAGAGVNVEMISTS
                     EIRISVVTRAEDAKAAVTAVHNAFGLDVDGQAVVYGGTGR"
     gene            11824..12873
                     /locus_tag="AC16Y1_04790"
     CDS             11824..12873
                     /locus_tag="AC16Y1_04790"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR5831810.1"
                     /translation="MSKKPTLAVVGATGAVGTVMLDLLSTRPDVWGEIKLLASARSAG
                     KVLQVRGKDVVVEELTEAAFDGVDVAMFDVPDEISAKWAPIAASKGVIAVDNSGAFRM
                     DKEVPLVVPEVNAEQTKNRPRGIISNPNCTTLSMIVVVGALEKAFGVKELVVSSYQAA
                     SGAGQAGIDTLQAQLDAIAGLGLGSSTKDVRNVITELGPFPAPLALNVVPWAGSLQEN
                     DYTSEELKVRNESRKILGRPDLKVHATCVRVPVVTTHSLAVHAVLNKNVTADEVRKLL
                     ADAAGVVVLDDPANKVFPTPIDVVDTDPTVVGRIRVSPEYPNEIDFFVCGDNLRKGAA
                     LNTAQIAEVVAAEFA"
     gene            complement(12881..13825)
                     /locus_tag="AC16Y1_04795"
     CDS             complement(12881..13825)
                     /locus_tag="AC16Y1_04795"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013865853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR5831811.1"
                     /translation="MGIEGVSESPRFWNAVTVGFLATTASTIGAVGLGTLSYSLFEAQ
                     SFTTREVTIPVLPKGSKDLRVLHLSDLHITPKQKRKIKWVRTLSQLDPDLVVVTGDFM
                     AHKDAVASVMYALGDLTSKPGFFVLGSNDYYAPYIKNPFMYFRKNREIKAEGKELPTK
                     DLVDSLINSGWFDLDNQQFRITVNGIAIHARGTDDPHINKDRYDLVAGPYDSFAFALG
                     VTHAPYRRVLNKFAADQADLVLAGHTHGGQVCIPFYGALVTNCDLPTGKAKGLSTFEF
                     EDQQMPLHVSAGVGTSPFTPIRLACRPEATLLTLTSKN"
     gene            13864..>14772
                     /locus_tag="AC16Y1_04800"
     CDS             13864..>14772
                     /locus_tag="AC16Y1_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831812.1"
                     /translation="MADDQSSPADNWDPLTSPYWEPLDNVEPIIAPPIEEFTTPEVQV
                     SRNIPQPPDVDEILRGEYTGWVPIIKPGDSVEAARIRFEQEQEKRQGSSEVMFEPKTE
                     QTLAAKVDPEDLNEFKELIREIKTEPDVYVKDFYSNLKDLAKDADTVASPAQALEEAR
                     RRIEQMRANLQVNLSRVQQSFEHPIFETKPVEEQITKFAQPEQAEEVVQVEQNQPKPE
                     FKSFELPEQTPVVFDYIPVETPITVEHVVEEVETEIHDEQIHHEEEPVQQTSQPIAQT
                     TVVNEEVKKEDHSLELVIMRDEIKDLR"
BASE COUNT         4194 a         3154 c         3410 g         4014 t
ORIGIN      
        1 aaagttgaat ttagatggcg ctcgaccttg gtcttggatt ttggcgtcac tttcgttagg
       61 tatggcattg ggtgtggtag tcgcaatgcg ggtaagacca aaacatccgt tggttcttgg
      121 tttactagcg caagttgctt tagtctcatt catgattgtg cttggcgtat ctaccaatgt
      181 ctggctaatt atgttgttcg cattcttctg cggtatcggc atggacttat ttatgattct
      241 ttggcagacc tcactgcaaa cacacattcc acgtgaatct ctgtcacgag ttgcctctta
      301 tgatgcgttt ggctcgctag ctcttgcgcc acttggttta gtcctagctg ggccagctgc
      361 cgaaagattt ggcgtggaaa cagccatcgt gacgctgggc gttatttgtg cgatcacttt
      421 ggctgcgatg atttcagttc ccggagttcg aaaactggaa gcaaaagagc cagaaaccgt
      481 ttaactgtgg ggttatgctt atctaagggt tcttgatcaa cccaaaagcc ttatttaagt
      541 ggctttagtg ccaacctacg ccggcgtagg ttacgctagc gtaggttacg taaccgtaag
      601 ttagcccaca gataatcccg gagttcaaat gaattcactt ggcttaccac ccattattca
      661 aggtggcatg ggtgctgcag tctcttcatg gcaacttgcc cacaccgtag ctagccttgg
      721 tcaactcgga gttgtttcag gaactgcatt agaaactgtt gttgctcgtc gcctacaaga
      781 tggtgacgaa ggtggacaca tgcgtcgcgc attagcggca tttccatatc aagatgtggc
      841 tcaacatgtg ctcgatactt tttatttacc tgaaggccgc aaaggtgaac catatcgacc
      901 gatgcgtcgc atttcgatta aagcgcataa agaacatgat caactttcag tcgctgcaaa
      961 ctttgttgaa gtttggttag ccaaacaagc aggcgctggc aaagttggaa ttaacttttt
     1021 agaaaaatta caaactgcaa ctcccgctgc actttacgga gcgatgctcg ctggggtaga
     1081 tgcaattttg atgggcgctg gaatcccgcg cgaaattcca caacttattc gtgatttcgc
     1141 agatggtaaa ccaggtcact taagtattga ttcagaacgc ccaagtggtg tcgatgcccc
     1201 aatacttgaa tttgatccgc tggaatcatt tggcgctgca ccaaaactaa atcgacctgc
     1261 attcttagca atcgttacag ctgaagtact tgctgcttat ctagcgcgca atgaaataac
     1321 aagacctgat ggatttgtag ttgagcatta ccatgcaggt ggacacaacg cacctcctcg
     1381 tcgaatgcaa gataccgaaa ctggttatgg accattggac gaaccaaaca tgcaaaaaat
     1441 tgctgacgtt ggtttaccgt tttggctcgc cggcggtcgg gcaactccgg aatctgttgc
     1501 agaagcgttc gcacttggag cctcgggtgt ccaagtgggt tcgctatttg cgctatcgaa
     1561 tgaatcaggt ttactgccta aatatcgtga acaaatgcta gatgctgcca gaaaaggtaa
     1621 cttgcgagta cgcaccgatc accgcgcgtc gccaactgga ttcccattta aagttgttga
     1681 acttcctggc acagtcggcg aagaatctgt ttatcaagca cgcccacgac tatgtgatct
     1741 tggctaccta agatcaagtc acatcgaaga tagtggcaag gttacttatc gttgttcagc
     1801 tgaacctgaa tcaccatttt tgaaaaaggg tggcgaagaa gatgacttac gcgcccgcat
     1861 ttgcttatgt aacggtttga tggcggcaat cggtctaggc caagagcgag ccgatggtta
     1921 caaagaagct ccacttttaa cacttggttc aacaactgga gatgttgagg caatgcttaa
     1981 agagtttcca aatggttggt ctgcagctga tgtagttaat cgactacttg ctagcgttcc
     2041 agcaacggca aaataattta gtgactttgt ttttttagtc gttcaaacga agccaaaatt
     2101 tccgcttcag cagaggcacg atcttcgaaa tgcgctcctt caactgactt accaggttct
     2161 aagtctttat aagtttcaaa aaagtgttga atttctaagc gatcaaattc tggaatgtct
     2221 tgaatatctt gtaaatggtt tttacgaatg tccttggcag gtacgcacat aattttgtcg
     2281 tcgccacctg cttcatctcg catgtggaac atgccgatag ctcggcaccg aattaaaact
     2341 ccaggaaaag tcggttcgtc aattaaaacc aaagcatcaa gtggatcgcc atcctggcca
     2401 agtgtgtttt caatgtagcc gtagtcgttt ggataacgtg tcgaagtgaa aagcatgcgg
     2461 tctaaacgta gtcggccagt ttcgtggtca acttcatatt tattgcggct accacctgga
     2521 atttcaacca gcacatcgaa ttcgagagcc acgctttgcc accaatcaac agttaaatac
     2581 gcttaattaa taggttaagt ctagtaagga aataaggcgt aaagtttcag caaatgatca
     2641 accttgaatt gcgttcggcc ttacttacta atttggcgga cttatcacca ggtgacgcta
     2701 ttgcagttgg cgttagcgga ggtgcagata gcgttgcact tttaaaagct gcagtgcatg
     2761 tcggtaaaga attaacccta aatgtgcacg ctgttgtgat tgatcatcaa cttcaaccaa
     2821 actcaaatga agtcgcaaac aaaactgccc agttagcaac agaactaggt gcaactgaac
     2881 ttgaggtcgt tcctgttcag gtagcaactg gaccgggaag cggtggcgtt gaagccgcag
     2941 ccagaaatgc cagacgccgt gcttttgaaa cttatgttgc aaaaaataaa atcgctgcag
     3001 ttttattagg tcacacactt gaagatcagg ctgaaacagt tctactcgga ttagctcgtg
     3061 ggtccggggc taagtcactt gctggcatgc aaagtattga tggaatttat cgccggccat
     3121 ttttaatgtt aagccgcgaa ttagttcgca gcactgtaag tgatttaccg acttatgaag
     3181 atccacacaa tagcgactta agttttgctc gagtgcgggt gagaaataac gttcttccag
     3241 ttttagaaaa agagctaggc ccgggtatta cgcaagcatt agctcgaaca gccgagatgt
     3301 tacgtgaaga tacccaagca cttgatagtt atgcgcagat gattctggct gccgaagatg
     3361 taggtatttc aacgctcgag gtattacctg ctgctgttcg tactcgcgtt attcgaaagc
     3421 tggctattaa ttccggtgta ttgattaatg acctaacccg tgaccatgta ttgaacattg
     3481 accagttaat tacaaactgg aaagggcaag gtccgctaaa tctgccaggc caagtaaatg
     3541 tgaaaagaga gtctggcaaa cttatctttt ataaaccttg aataggagta tttgtggacg
     3601 cagcttcaat tggcgatgaa ctaactagtg tggtgctgac tgaagagcaa ttgcgaactc
     3661 gcatcaagga aattgctgcg caaatcgata atgattatgc gaacttagat aaaccactac
     3721 ttttagttgg tgtactaaaa ggtgcagtca tggtgatggc tgatttagcc agggcaattt
     3781 cgattcctgt gcaattcgat ttcatggcag tcagttctta tggatctgga accaaatcaa
     3841 gtggcgtagt tcgaattatt aaagatctag atatcgatct aaccggtcgg cacgttttaa
     3901 ttatcgaaga catccttgat tctggtttaa ctctttcctg gttaattaaa aacctcaagt
     3961 cacgtggccc ggtttcaatc gaagttatga ccatgatgcg caaacctgat gcaataaagg
     4021 ttgaagtaaa tgcacgttac attggttttg atattccgaa cgaattcgta gttggctttg
     4081 gacttgatta tgcagagcgt tatcgaaacc tacgcgaagt gggagtatta gccccacacg
     4141 tttacggcgg ctagtttttc aatttcacat ggtgaaaatt cggattagca acggattctt
     4201 actgttggta accttgcctt aacttacaaa tgagatttaa aagttctacc tattaataca
     4261 gtcctaggag ataaaaatgg acaattcagc taaatgcccg gttgagcatg ggggccaaac
     4321 aaaagctggt tactcaccga tggattggtg gccaaatgca cttaaccttg aaatcctgca
     4381 ccaacatgac accaagacaa atccacttgg cgaagatttt gattaccgca aagaagtaaa
     4441 gtcgttagat tttgctgcac ttaaaaaaga tctacatgaa ttaatgacgt caagccaaga
     4501 ttggtggcca gctgactggg gtcattacgg cggcttgatg attcgtttgt cttggcacgc
     4561 tgctggtaca tatcgcgtat ctgatggtcg cggtggtgcc ggtacaggta gcaaccgatt
     4621 tgcgccgctt aactcatggc cagataacac caacttagat aaagctcgtc gtttactttg
     4681 gccaattaaa aagaagtacg gcaacaaaat cagttgggct gacttactag ttcttgctgg
     4741 aacgattgcc tacgaatcaa tgggtcttaa gacttacggt tttgcttttg gtcgtccaga
     4801 tatttgggat cttgaaaaag atgtttattg gggttcagaa aaagaatggt tagccccatc
     4861 agatgagcgt tacgaaaacg ttgacgatgc ttcaacaatg gaaaatccac ttgctgcagt
     4921 gcagatgggt ttgatctatg taaatcctga aggcgttaac ggaaaaccag atccactaaa
     4981 gactgcccaa catgtgcgcg aaacatttgc tcgcatggca atgaacgatg aagaaactgt
     5041 tgcactaact gctggtggtc acactgttgg taaagcacac ggtaacggtc gggccgaaaa
     5101 cttaggtcca gaacctgaag gtgccgacat ttctgaacaa ggtctgggtt ggaataacca
     5161 caagactcgt ggcattggta acagcactgt tacttcaggc attgaaggtg cttggaccac
     5221 taatccaact aaatgggata acggttactt tgacttgtta tttaaatatg aatgggaatt
     5281 aaagaagtct ccagcaggtg caaaccaatg ggaaccaaag aatatttccg aagaagacaa
     5341 gccagttgat tcagaagatc cgactaagcg ttacaacccg atcatgactg atgcagacat
     5401 ggcaatgatc aaggatccga tttatcgtga aatttctgaa cgtttccgaa acgatcagga
     5461 ttacttctca gatacatttg ctcgcgcttg gttcaagcta actcatcgtg acatgggtcc
     5521 aaaagttcgt tacatcggac cagatgtccc agctgaaaat ttaatttggc aagatccaat
     5581 tcctgctggc aataagaatt atgacgttgc ggccgtacgc aagaaaatcg aagcaagcgg
     5641 gctttcagtt tcgcaaatgg tttcaactgc ttgggatagt gctcgcacat tccgtggttc
     5701 agatatgcgt ggtggtgcca atggtgcgcg cattcgctta gctccacaaa aagattgggc
     5761 tggcaacgaa ccagagcgtt tacaaaaggt gcttgcaaca cttgaaccga ttgccaaaga
     5821 aactggcgct agcctggctg acgtaattgt gttggctgga aacattggcg ttgagaaggc
     5881 agctaaagca gccggttttg atgtaaacgt tccatttgct ccaggtcgtg gcgatgcaac
     5941 tcaagaacaa acagatgttg aatcatttgc tgtacttgaa ccacttgctg atggtttccg
     6001 taactggttg aagaaagatt atgtagttag tccagaagag ttactacttg atcgcgctca
     6061 gttaatgggt ctaactggtc atgaaatgac agcactgtta ggcggtatgc gagtaattgg
     6121 taccaatcat ggtggcgcta agcacggtgt gttcacagag aacgttggcg cattaaccaa
     6181 tgatttcttc gtgaacttaa ctgacatgat ttatgtctgg gaacctgccg gtaataacct
     6241 ctacaacatt cgcaatcgca agactggcga agttaaatac actgcaactc gagctgactt
     6301 agtgtttggt tctaactcga ttttgcgtgc gtacgccgaa gtttatgcac aagatgacaa
     6361 caagaagaag tttgttaatg acttcgttgc tgcttgggta aaggttatga acgccgaccg
     6421 tttcgatcta gaataaatca ctaaaaccaa aaacagggcg ctggattcca gcgccctgtt
     6481 ttatttgagc tgaattaaat ttgataagga gtgacttttg cgattagccc aattggaatt
     6541 ggtgcgtaaa tgtgagggaa gagttggcca ttgcgacttt cttctacttt cacttccagg
     6601 cctgcgctta ttagcgttcc gatcttcatt tcaagtaata caagttcatc cgtagatgct
     6661 gagtaatgca gtctggctgt ttcagctagt tgatcaggaa aagatgcatg aataaatccc
     6721 acttcttcta aagtttgatc caaagttgat tgggtgtaga aacctttttc gacagcagct
     6781 tcccaaacag gttttgtagt taagtgcaga attgtggatg aagtcatgga ataagtatgt
     6841 atggggtgat ggcgacttcc tctggcggag gataggggaa ccgatctttt attttccgat
     6901 taagttgatc caatttgatc aacgaatcga cccccaaatc ccgtttgtct catgaaaata
     6961 aaaaacaccc cggaatcgga gtgtttttta tttgcgcgga ggatagggga tttgaacccc
     7021 tgagggcttg cacccaaccc gctttccaag cgagcgccat aggccactag gcgaatcctc
     7081 cgtgaaagac cttacctgca aattgatacc cgacgctaat tgccaaagtc gtgcgagaca
     7141 atctattcat gcgaattgga gttattggtg ttggaacaat ctccaccaat gtcatcgaag
     7201 caatgcaaaa gctaaatact tcacatactt tttacttatc gcctcgctca aatcagcgtg
     7261 ctaaagattt ggcaagtaaa tataagaact gtattcaagt aaattcaaat caaggagtag
     7321 tcgacaacag cgacatagtt attgttagcg ttttaccaaa gcaagttgat gaagttctaa
     7381 ctgaattaac ttttcgacct gaccaaataa ttactagttt tattgccggt cgtccacctt
     7441 cagaaatttc aaaattaaca gtgccggcaa caaaagtagc ccaacttatt ccgttgccgc
     7501 caattgagtt acataaaggt ccgttattaa tctgtcccgg aattccagag ctgatcgaaa
     7561 ctttttctgg acttggtgat gtaattccgt ttgatgatga acaaaacatc aaagttttta
     7621 gttcagcatc tgcagtcatg tccacttact tcagacttgt acttgaaata gttgattgga
     7681 ttactgccca aggaattgat cgaaccttag cgaccaaata tatgacttca ctttttgaag
     7741 gactgggaac agaatcaaac cacatcgcac cagatttgtt gacgtctatg gttcgtgaac
     7801 atgaaactcc aggtggttta aatgaatcgg ttcgaactaa tttagagaaa tctgaatggt
     7861 acgccgatgt tcgcgcacaa ttggattatt tgatcaaagt gtttattcca gccttgaaaa
     7921 atcagaaata atttcggcaa aatttcttaa ctagacttgt ggctagcccc tcacgtggcg
     7981 taaccctgtg aacctcccca ggaccggaag gtagcaagga taagcgggct ctttcgggta
     8041 cgtgaggggt cctttaatgc cagcctgtct tagtcaacga ctaatcttgc tttatgtcct
     8101 tggcgcttta ccgaaaatac cgacccggtc agttcgctga tgtaatcggc caagaacatg
     8161 taactgttcc actatctagg gcactcgata atgatcgact acatcatgcc tacctattta
     8221 cagggccacg tggatgtggc aaaacttcat cagcacgcat catggctcga tcaattaact
     8281 gcgcgaatgg tccgacttca actccatgtg gagtttgcca aagttgcatc gacttagcac
     8341 caaatggccc aggttcaatc gatgtaattg aaattgatgc agcaacctat cgcggtattg
     8401 atgatgcaaa agaattgcgt gaacgagcag tttatgcacc ggctaattcg cgttacaagg
     8461 tttacataat tgacgaggca catcagctaa cccgtgatgc tttcaatgtt cttctgaagt
     8521 tagttgaaga accaccacca catttgcgct ttattttcgc aactactgaa ccagacaaaa
     8581 ttattccaac tctgcgctcg cgtacgcacc attatccgtt tcgattggtt tcagcaaaaa
     8641 acttagctga acacctcggc ataatttgcc agcgcgaagg agttaaagca gatccagcat
     8701 cacttgcact tgtggcaaga gctgcggccg gatcagttcg tgatgcccaa tcaattcttg
     8761 gccaggtaat tgctggtgcc ggtagcgaag gaataactta tagcgaaaca gtggcacagc
     8821 ttggcttcac cgatgaacgc ctattaggtg atgtggttgt tgcaattgca aaaggtgatg
     8881 gcgcaaaatt atttacactt gttgatcaag ttgtgacaac aggtcatgaa ccacgcagat
     8941 ttgtggctga tttacttgaa cgtcttcgtg atttattgat tgtcacgcag attccagatt
     9001 cagcaaactc aggattattt gatttaccag ctgaacaagt ttctgattta gttgagactg
     9061 caaaattatt tacttatggt caactagtgc gcgttgctga tttagtgaat tctggcatga
     9121 gtgaattgcg cggagctgca tctcctcgtt tgcaacttga acttttagta gccagattag
     9181 tcacgcctga agttgcgcaa gatctagcgc agcgagttgc caaacttgaa caaggtggag
     9241 cgccaagtta tcaacgtccg gtcagtgaac ctgctgccgc aaaaacgcag gccgttgaac
     9301 cgaaagtaac tccgacagct ccaaaagcgg cgccagttgc tagaactcaa gatgcaccac
     9361 caccaatgaa accttcgcaa gttgctgctg cagcgcacgt taaggcaact gaagaaaaac
     9421 ctgttgaagc aaaaccacaa ggtgaaatta ctttcacttt acttcgcgca aactgggtcg
     9481 gaatattaga aactctaaaa actcaaagcc gagttgcttg gttagtactt caagacactg
     9541 tgccagtttc gattagcgat gaccgcattg tgaccgttgc atttagtagc gcaggtgcga
     9601 tgaataattc aatcaagagt aaacatgatg aaaaacttcg tgatgcgatt aagaaagttt
     9661 tgaatgccga tgtgcgtatt gaaggaacag tagatccaaa acacgctaaa tcaccggcag
     9721 ctaaaaaaga agttgccgac gcaggtgaag catcaccaga tgatgaagca gtaactaagc
     9781 gttcggcttt agacatggtt actaacatgc ttggtgctga aatcatttct gaaagtcctc
     9841 gccaataatg agcaacattt atgaaggcat agttcaagat ttaatcgacg aatttgccaa
     9901 gttacccgga gtcggtccaa aaggtgctac ccgaattgca tttcatttac taaattcaga
     9961 tcaccatgat gtcaccagac ttgccgaagt attacgagaa gtaaaagaga aagtcagatt
    10021 ttgcaaagag tgctacaacg ttgcacaaaa tgaacattgc cgaatctgcg cagacaatcg
    10081 acgggatcaa acttcaattt gcgtagttga agaaccaaaa gatgttttag cagtagaacg
    10141 cacccgcgaa tttcgcggta agtatcacgt gcttggcgga gcaatcagcc cgatagacgg
    10201 tattggtcct gatgatttgc ggatcaagga attaatgtcg cgccttgcaa gtaacagtgt
    10261 ttccgaagtt attttggcaa ctgatccaaa tctcgagggt gaagcgacgg caacgtattt
    10321 agctcgacta ctttcaccac ttggactgaa agtaactcgc ttagcaagtg gactgccagt
    10381 aggtggcgat cttgaatatg cagatgaaat aacacttggc cgtgcttttg aaggccgccg
    10441 ccaagtttca ccttaatttt taatctgccc acatttcaga caaaaaaatc tgacatttaa
    10501 tttgccaata gaattaaggg ctatgagcca aatcgtcatg aaatttggcg gctcatctgt
    10561 tgcagatgcc gccagcgttc tgcgtgtggc aaaacgaatt gttgataata aaaatgctgg
    10621 caatgaagta gttgtaattg tttcggcgat gggcgacacc accgatgaat tactcgattt
    10681 agcaaaagaa gtttcgccaa atccgcctgg tcgcgaattg gatatgttgc taactgctgg
    10741 cgagcgaatt tcgatggccg tacttgcaat ggcaatcagt gatctaggcg ttgaagcccg
    10801 ttcttacact ggttcacaag ctggactgat taccgactca acccacggta aagcgcgcat
    10861 tgttgatgtg actccgggtc gtattaaaga agcacttgca gatggcgcaa ttccaatcat
    10921 tgccggtttt caaggtgttg ctcaagatac aaaagacatc accacacttg gccgtggtgg
    10981 ctctgactta actgcagtgg caattgctgc cgcacttaat gccgatgttt gtgaaattta
    11041 tactgacgtt gacggaattt tttcagccga tccgcgcatt gcgccaaaag cgcgccaagt
    11101 tccgcgcatt acttacgacg aaatgatgga gttagctgca gctggcgcca aagtactaca
    11161 tctacgctgc gttgaatatg caaagcgttt tgatgtgcca atccatgtgc gctcttcttt
    11221 ttcgcagcgc gaaggaactt ttgttattgc tgatttatct gatcagtcaa agattcaaaa
    11281 actgtcacag caaggagctg ttatggaaca accaattatc gccggtgtcg ctcatgatct
    11341 aaacgatgcc aagattactg tcgttgctgt gccggataaa ccaggtactg cagcggcaat
    11401 cttccaagcg gtggcagatt caggcatcaa catggacatg attgtgcaga acgtgagtgc
    11461 tagcggtgcg acagcaactg acattacatt tactgtgcct cgtgttgatg cagcaaaagc
    11521 agaacttgct ctgcgcaaag tgcaaagtga aattggtttc aaagatattg atactgacga
    11581 aaacattgcc aaaatttctt tagttggcgc tggaatgcgt tcacatcctg gtgtttcagc
    11641 tacattcttt aaagcaattg ctggtgcggg tgtgaacgtt gaaatgattt caacttcaga
    11701 aattcgtatc tcagtagtaa ctcgagccga agatgccaag gctgctgtca ctgcagtgca
    11761 caatgcattt ggtttagatg tagacgggca agcagttgtt tacggcggaa cgggtcggta
    11821 attatgagta aaaaaccaac tttggcagta gtcggcgcaa ctggcgcagt cggaacagtc
    11881 atgctcgact tactatccac tcgcccagat gtttggggcg aaattaagtt attggcctcc
    11941 gctcgctcag ccggaaaagt tttacaagtc cgcggcaaag acgttgttgt cgaagaatta
    12001 actgaagctg cttttgacgg tgtcgatgtt gccatgtttg acgtgccaga tgagatctct
    12061 gccaagtggg caccgatcgc tgcaagtaaa ggcgtaattg ccgttgataa cagtggcgct
    12121 tttcggatgg ataaagaagt tccgcttgtt gtacctgaag taaacgctga acaaactaag
    12181 aatcgtccgc gcggaattat aagtaatcca aactgcacca cactttcgat gattgttgta
    12241 gttggagcac tagaaaaagc atttggcgtt aaggaactag ttgtttcttc ctaccaagct
    12301 gcctctggtg ctggccaagc tggcattgat actttgcagg cccaacttga tgcgattgca
    12361 ggtttaggac ttggttcatc aactaaagat gtgcgaaatg taataactga acttggcccg
    12421 ttcccagcgc cacttgctct aaacgttgtg ccatgggcgg gatctcttca agaaaacgat
    12481 tacaccagcg aagaattaaa agttcgcaat gagtcacgca aaatacttgg tcgcccagat
    12541 ttaaaggtgc atgcaacttg tgttcgcgtt ccagtggtta caacgcactc acttgcagtg
    12601 catgcagtac ttaacaaaaa tgtaaccgca gatgaagttc gtaaacttct ggctgatgct
    12661 gctggtgtag ttgtactaga tgatccagca aataaagttt tcccaactcc gattgatgtt
    12721 gttgatactg atccaacagt tgtcggccgt attcgcgtct cacctgagta tccaaatgaa
    12781 attgatttct ttgtttgtgg tgacaactta cgaaaaggtg ccgctctcaa cactgcacaa
    12841 atcgccgagg ttgttgccgc agagtttgct tagagtttaa ttaattcttg gaagttaacg
    12901 taagtaaagt tgcttcaggt cggcaagcca atcgaattgg cgtgaacggt gacgtaccaa
    12961 ctcctgcgga aacatgtaat ggcatctgtt gatcttcaaa ttcaaaagtc gataagcctt
    13021 tggctttgcc agttggcaaa tcacaattcg taactaatgc accgtaaaac ggaatacaaa
    13081 cttggccacc atgtgtgtgt cccgctaaaa ctaaatcagc ttggtcagcg gcaaatttat
    13141 taagcactcg tcgatatgga gcgtgggtaa caccaagtgc aaaagcaaat gaatcataag
    13201 gaccagccac taagtcatag cgatctttat ttatgtgcgg atcatcagtt ccacgggcat
    13261 gtatcgcaat tccgttaacg gtgatccgga attgttgatt atctaagtca aaccatcccg
    13321 aattgattaa tgagtccact aaatcttttg ttggtaactc ttttccttca gctttaattt
    13381 cacgattttt acggaagtac ataaatggat ttttaatgta tggcgcgtag taatcattac
    13441 tgcccaatac aaaaaaaccg ggcttgcttg ttaggtcacc aagtgcgtac ataactgagg
    13501 ccactgcatc tttatgtgcc atgaaatcac cggttaccac aaccagatcg ggatcaagtt
    13561 gggaaagtgt tctgacccat ttaatcttgc gcttttgttt tggcgtgatg tgtaaatcag
    13621 ataaatgaag tacccgtaaa tctttactgc ccttgggtaa aaccgggata gtaacttcgc
    13681 gggttgtgaa agattgggct tcgaacaagc tataactcag cgtgcccaga ccaacggcac
    13741 caattgtgct ggcggtcgtt gctaaaaaac ccacagttac agcattccag aatctgggac
    13801 tttcgctaac cccctctatt cccatatgct tgtaactctt ctttctttcc ccaaggaggc
    13861 actatggctg acgatcagtc cagccctgcc gacaattggg atccgttaac gtcgccatac
    13921 tgggaaccac tcgacaacgt agagccaatc attgctccgc caattgaaga attcacaact
    13981 cccgaagttc aagtttcacg taacatcccg caacctccgg atgttgatga aattttgcgc
    14041 ggcgaataca ccggttgggt accaattatt aaacctggtg attcagttga agctgctcgc
    14101 attcgctttg aacaagaaca agaaaagcgt caaggttcat ccgaagtaat gtttgaacca
    14161 aaaaccgaac aaactttggc tgcaaaagtc gatccagaag atttgaatga atttaaggaa
    14221 ttaatccgcg agattaaaac tgagccagat gtctatgtaa aagattttta ttcaaactta
    14281 aaggatttag ccaaagacgc cgacaccgtc gcatcgccag cgcaagctct agaagaggct
    14341 cgccgtcgga ttgaacagat gcgagcaaat ctgcaagtta acttgtcgcg cgtacaacaa
    14401 agttttgaac atccaatctt cgaaacaaaa ccagtcgaag aacaaattac gaaatttgct
    14461 cagcctgaac aagcagagga agttgttcag gttgagcaaa accaacctaa gcctgagttc
    14521 aaatcttttg agcttccaga acaaacacca gttgtatttg attacatccc ggtagaaact
    14581 ccaattaccg ttgaacatgt agttgaagaa gttgaaactg aaattcacga tgaacaaatt
    14641 catcacgaag aagagccagt tcaacaaaca agtcagccaa tagcgcaaac aactgttgtt
    14701 aatgaagaag tgaaaaaaga agatcactca cttgaattag tgattatgcg tgatgaaatt
    14761 aaagatttgc gc
//
LOCUS       DBNHVN010000252         1063 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1533054, whole genome shotgun sequence.
ACCESSION   DBNHVN010000252 DBNHVN010000000
VERSION     DBNHVN010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1533054"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="AC16Y1_04805"
     CDS             complement(<1..653)
                     /locus_tag="AC16Y1_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004931998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR5831813.1"
                     /translation="MDKQTEFVLRSMEERDIRFVRLWFTDVLGTLKSVAIAPAELEGA
                     FAEGIGFDGSAIEGFARVYESDMLAKPDPETFSILPWRGEQNSVARMFCDINLPDGSA
                     SFADPRNVLKRALGKAADLGFTFYTHPEIEFFLFKNQPEKGVTPIPVDEVGYFDHSPS
                     GVGYDFRRQAITMLEAMGISVEFSHHEGAPGQQEIDLRYADALTTADNIMTFRVVMK"
     gene            714..>1063
                     /locus_tag="AC16Y1_04810"
     CDS             714..>1063
                     /locus_tag="AC16Y1_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR5831814.1"
                     /translation="MNTIRLALAQVNPTVGDFAGNLNLCFDSVLKANEQGVQLVLLPE
                     MVITGYPIDDLALRPSFQKASNEAIQELATKLVQENLGHIVVVVGYLRGTDVNDAAKL
                     GVPRGAPMNSAAVI"
BASE COUNT          296 a          251 c          225 g          291 t
ORIGIN      
        1 tccttcatca caactcgaaa agtcatgatg ttgtctgcgg tagtcagagc atcggcataa
       61 cgtaaatcaa tctcttgttg acctggtgca ccttcgtgat gactgaattc aacagatata
      121 cccatggctt ctaacattgt gattgcttgc cgacggaagt cgtagccaac accacttggc
      181 gaatggtcga agtaacctac ttcatcaact gggattggag ttactccttt ttccggctga
      241 tttttaaata agaaaaactc gatttctgga tgtgtgtaaa aagtaaaccc aaggtctgct
      301 gctttaccaa gtgctcgctt taatacattc cgcggatcag caaatgaagc actgccatct
      361 ggcagattaa tgtcgcaaaa cattcttgcc acactatttt gttcacctcg ccacggcaga
      421 atagaaaatg tctcaggatc tggtttagcc aacatgtcag attcatacac acgggcaaag
      481 ccttcgatag cagaaccatc aaatccaata ccctctgcaa atgcaccttc gagttcagct
      541 ggagcaattg caacagattt aagtgtgcca agcacatcgg taaaccaaag tcggacaaag
      601 cgaatatccc gctcttccat tgaccgtaat acgaattcag tttgcttatc catagtccct
      661 atgttcctcg gctttcgtta cagccgtgtt acaagcccga tatgctgata tccgtgaaca
      721 ccatccggtt ggccctggca caagttaatc ccacagttgg tgattttgct ggaaacttaa
      781 acctttgttt tgattctgtt ttgaaagcta atgaacaagg tgtgcaacta gttttattac
      841 ccgaaatggt gattaccggt tatccaattg atgacctggc ccttcggcct tcgtttcaaa
      901 aagcaagtaa cgaggcgatc caagaattag ccaccaaatt agtgcaagaa aatttaggtc
      961 acatagttgt cgtggttggt tatttgcgcg gcacggatgt aaacgacgcc gccaaattag
     1021 gtgtaccgcg aggggcgcca atgaatagcg ctgccgtaat tca
//
LOCUS       DBNHVN010000253         3784 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_938783, whole genome shotgun sequence.
ACCESSION   DBNHVN010000253 DBNHVN010000000
VERSION     DBNHVN010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3784)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3784)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3784
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_938783"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1114
                     /locus_tag="AC16Y1_04815"
     CDS             <1..1114
                     /locus_tag="AC16Y1_04815"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005458293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HIR5831815.1"
                     /translation="LPVLLERSRIEKEAQIEIVEALETFPSTLPARRDAAHSGWVSVS
                     VGCNNTCTFGIVPALRGREKDRRPADILNEIKVLVEQGVLEVTLLGQNVNAYGVEFGD
                     RYAFADLLKATGQIEGLERVRFTSPHPRDFTDHVIEAMATTYNVMPQLHMPLQSGSDR
                     ILQIMRRSYRQEKYLGIIERVRKAIPDAAITTDIIVGFPGETEDDFQQTLEVVKQARF
                     AGAFTFQYSKRPGTPAAEMPDQIDPEVVQERFERLVELANDIAWQENKNLVGTQVEVL
                     ISTGEGRKNENSGRMSGRARDNRLVHVTQTDITGVTHEIRPGDIVTAEVTYAAPYHMN
                     ADKLLSVRKTRAGDNYEAGTRPTTPGVLLGMPKIGG"
     gene            1114..2013
                     /gene="miaA"
                     /locus_tag="AC16Y1_04820"
     CDS             1114..2013
                     /gene="miaA"
                     /locus_tag="AC16Y1_04820"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019547269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR5831816.1"
                     /translation="MQPAIVIVGPTAVGKTQLSLDVAHEFSGEIINADSMQLYKGMDI
                     GTAKLPPAQRRGIKHHMLDVLEVTQSANVADYQRDARQIVQELDVAGVRSVIVGGSGL
                     FVQALLEDMQFPGSDPEVRERLTNEAEEIGPEAIYQRLVEIDPQAAANILPGNTRRVI
                     RALEVIELTGLAPVTTLQQLNEVIPSIRIGLSRDRADLDERITMRVDLMWEQGFVAEV
                     EELEKQGIREGLTASKALGYAQVLPALAGEITLEEAKAQTIQATKRFARRQESWFARD
                     KKIHWLDATSATLDDVAQLVNKN"
     gene            2044..2901
                     /gene="dapF"
                     /locus_tag="AC16Y1_04825"
     CDS             2044..2901
                     /gene="dapF"
                     /locus_tag="AC16Y1_04825"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR5831817.1"
                     /translation="MTKSPTPIGAPFLKGHGAGNDFIVLDATTGGLNPSAKAVEVLCD
                     RRKGIGADGILRLAPASEFNVTDANYFMDYRNSDGSLGQTCGNGLRVVARFLVEQGYE
                     QAGSFQIGTRAGTVTAHVAPDDTNFENIAINMGTFAQLNRDLTVSTETGSWQASGIES
                     PNPHAVSIVGSIWDAGSLSDYPSVTPDGTFPDGVNFEFVEPKSPTHIAMRTHERGVGE
                     TLACGSGSCAAAQVWAIKNGLEPGWSVQVDLLGGTVFVDQDLTGSLILRGGAQIVATG
                     NISPQLWQA"
     gene            2904..>3784
                     /gene="hflX"
                     /locus_tag="AC16Y1_04830"
     CDS             2904..>3784
                     /gene="hflX"
                     /locus_tag="AC16Y1_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003935621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR5831818.1"
                     /translation="MTDNFDTELTIGQQDLESRAALRRVAGLSTELQDITEVEYRKLL
                     LEKVVLIGVWSSGSYDQATRSMAELAQLAETAGSQVMDALIQRRDTPDPATYIGSGKL
                     AELRQIVVATGADTVICNGELSPSQLSKMEKVVKVKVVDRTWLILDIFAQHARSREGK
                     AQVSLAQMQYMLPRLRGWGESLSRQSGGRVGSQGGGIGTRGPGETKLETDRRRIGKSM
                     AKLRREIKEMHKARLTQRKAREKHKVPAVAIAGYTNAGKSSLLNRLTNAGVLVENALF
                     ATLDPTVRQAKTPSGRE"
BASE COUNT         1064 a          842 c          941 g          937 t
ORIGIN      
        1 acttccagtg ttacttgaac gttcgcgaat tgaaaaagaa gcgcagattg aaatagtcga
       61 ggccttagaa acatttccat cgactctgcc ggctaggcga gatgcagcgc actcaggctg
      121 ggtatcagta agtgttggtt gtaataacac gtgcacattt ggtattgtcc cagcgctgcg
      181 tgggcgtgaa aaagatcgtc gtccagccga cattttaaac gaaataaaag tactagttga
      241 gcaaggcgtt ctcgaagtta cgctacttgg gcaaaatgta aacgcatacg gcgttgaatt
      301 tggtgatcgc tatgcattcg cagacttact taaagccact ggtcagatcg aaggcttgga
      361 gcgagtgcgg ttcacatcgc cgcatcctcg tgacttcacc gatcatgtga ttgaggcgat
      421 ggctactaca tataatgtga tgcctcagtt gcacatgcct ttgcaatcgg gcagcgatcg
      481 cattttgcag attatgcgcc gctcataccg tcaagaaaaa tatttaggaa ttatcgaacg
      541 ggttcgaaag gctattccgg atgcagcaat caccaccgac attattgtgg gcttcccagg
      601 tgaaaccgaa gatgatttcc agcaaacttt agaagttgtt aaacaagcac gttttgctgg
      661 tgcgtttact ttccaatatt caaagcggcc gggaacgcca gctgcagaga tgcctgatca
      721 aattgatcca gaggttgttc aagagcgatt tgaacgccta gttgaattag ccaacgacat
      781 cgcttggcaa gagaacaaga atctagtcgg tacccaagtt gaggttctaa tctcaactgg
      841 cgaaggacga aagaatgaga actcaggtcg tatgagtgga cgcgctagag ataatcgctt
      901 ggtgcatgta acccaaaccg acataactgg agttacccac gaaattcgcc caggtgacat
      961 cgttaccgcc gaagttactt atgcggcgcc gtatcacatg aatgcggaca agttactctc
     1021 agttcgcaaa actcgtgcag gcgataacta tgaggcaggc actcgaccaa ctacaccagg
     1081 tgtattactt ggtatgccaa aaatcggcgg ctaatgcaac ctgcaatagt tattgttggt
     1141 ccaactgccg taggtaaaac gcaattgtca ctcgatgtgg cacatgaatt ttccggcgaa
     1201 attattaacg ctgattccat gcaactttat aaaggtatgg acatcggtac agccaaactt
     1261 ccaccagcac agcgacgcgg gataaaacat cacatgcttg atgtgctcga ggttactcag
     1321 tcagcaaacg tagcggatta tcaacgtgat gctaggcaaa tagttcagga actagatgta
     1381 gccggcgtta gaagtgtcat cgtcggtggc tcaggattat tcgtgcaggc cctgcttgaa
     1441 gacatgcagt ttcctggaag tgatccagaa gttagggaac gcctgactaa cgaagctgag
     1501 gaaattggac cagaagctat ctatcaacgc ttagttgaaa tagatccgca ggcagctgcc
     1561 aacatactcc ctggaaatac ccgtcgagta attagagcac ttgaagtaat cgaactaact
     1621 gggcttgcgc cggtaactac tttgcagcaa ttaaatgaag taatcccaag tattcgaatc
     1681 ggccttagtc gagatcgcgc tgatttagat gagcgaataa caatgcgagt tgaccttatg
     1741 tgggagcaag gttttgttgc cgaagtcgaa gaacttgaaa agcagggtat ccgggaaggt
     1801 ttaaccgcaa gtaaggccct tggctatgcg caggtgcttc ctgctttggc aggtgagata
     1861 acccttgaag aagctaaagc ccaaactatt caagccacga aaagatttgc gcggcgccaa
     1921 gaatcatggt ttgcccgaga taaaaaaata cactggctag acgccaccag cgcgactttg
     1981 gatgacgttg cgcaattagt gaacaaaaac tagacaaata ctgcctcgcg tagaattaac
     2041 tcaatgacta aaagccccac tccgatcggc gcacctttcc ttaaaggcca tggtgctggc
     2101 aacgatttca ttgtgctcga tgcaacaact ggtggcttaa acccaagcgc aaaagccgtt
     2161 gaagttcttt gcgatcggcg caaaggtatc ggtgccgatg gtattttgcg actggcaccg
     2221 gcaagtgaat tcaacgtaac ggacgccaac tattttatgg attaccgaaa tagcgatggt
     2281 tcacttggac agacctgtgg caatggactt cgggtcgttg ccagattttt agtcgaacaa
     2341 ggttatgagc aagcaggaag ttttcagatc ggtactcgtg ctggaactgt aacagcgcac
     2401 gttgctcccg atgatacaaa tttcgaaaac atcgcaatca acatgggcac atttgcgcaa
     2461 cttaatcgtg acttaaccgt aagcaccgaa actgggtcat ggcaggcaag tggaatcgag
     2521 tcaccaaacc cgcatgcagt atcaattgtt ggaagtattt gggatgccgg aagtttgagc
     2581 gattatcctt ccgtgactcc cgatggcact tttccagatg gcgttaattt tgagttcgtt
     2641 gaaccaaaaa gcccaactca tattgctatg cgaactcatg aacgcggagt cggtgagacg
     2701 ttggcttgcg gttctggttc atgcgctgca gctcaagttt gggcaattaa gaatggatta
     2761 gaaccaggtt ggtcagttca agttgatcta cttggtggca cggttttcgt cgatcaagat
     2821 ttaactggat ctttaatatt gcgtggcggc gcacaaatcg tagctaccgg aaacatttcg
     2881 ccacaattgt ggcaggccta accttgacag ataatttcga taccgaatta actatcggcc
     2941 aacaagatct tgagtcacgc gctgccttaa ggcgcgttgc tggattatct actgaacttc
     3001 aagacattac cgaagttgaa tatcggaaat tacttttaga aaaagttgtg ttaatcggag
     3061 tttggtcatc tggtagttac gatcaagcga ctagatcaat ggccgaactt gcgcagttgg
     3121 ctgaaactgc tggctcacaa gttatggatg cgctaatcca gcgtcgcgat actcccgacc
     3181 cagccaccta cattggttct ggcaagttag ccgaacttag gcaaattgtt gtggcaactg
     3241 gcgcagatac cgttatctgc aatggtgaac tttcaccttc gcagttatcc aaaatggaaa
     3301 aggtagtaaa agtaaaagtt gtagaccgta cttggttaat tttggatatc tttgctcaac
     3361 atgcacgcag tcgtgaaggt aaagcacaag tgagtttggc gcaaatgcaa tacatgctgc
     3421 caagacttcg cggttggggt gaatctttat ctcgccaatc tggtgggcga gtgggttcac
     3481 agggcggtgg cattggtacc cgcggtccag gtgaaaccaa acttgaaacg gatcgtcgtc
     3541 gcattggtaa gtcgatggct aaacttcgcc gtgaaattaa agaaatgcat aaggcccgac
     3601 ttactcaacg caaagctcgc gaaaaacaca aagttcctgc agttgcaatc gctggctaca
     3661 ccaacgccgg caaatcaagt ttgttaaatc ggctaacaaa tgcaggcgta ttagttgaga
     3721 atgcactctt tgccactctt gatccaacag ttcgtcaagc caaaactcca agtggtcgtg
     3781 aatt
//
LOCUS       DBNHVN010000254         1538 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1459977, whole genome shotgun sequence.
ACCESSION   DBNHVN010000254 DBNHVN010000000
VERSION     DBNHVN010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1459977"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="AC16Y1_04835"
     CDS             complement(<1..503)
                     /locus_tag="AC16Y1_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009720263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5831819.1"
                     /translation="MTQKLLNPHADVVTLTSQLIDIPSVSGNEAQIADLVEGALRACD
                     WLTVERINNNVIARTNLGRSSRVIVAGHLDTVPVAENDKAVLLKAGQQLPVEGSTLAE
                     DVLFGLGSCDMKGGVAVALRAAVTLTNPVFDVTYIFYECEEVDSARNGLTKIAAQKPE
                     WLAADIAV"
     gene            609..1529
                     /gene="dapD"
                     /locus_tag="AC16Y1_04840"
     CDS             609..1529
                     /gene="dapD"
                     /locus_tag="AC16Y1_04840"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HIR5831820.1"
                     /translation="MTNLSASAIGLATYSGDTLLDVWYPNPTLSATPAKVSDLTSSAN
                     SDELRQVRSEVITTVVSDLSTPPIDAADVYLRLHLLSHRLVKPRSINLDGIFGLLQNV
                     AWTSLGAVAIDKLTEVQISARAGGLSFNVLGVDKFPRMVDYVVPSGVRIADADRVRLG
                     AHLAEGTTVMHEGFVNFNAGTLGTSMVEGRISAGVVVGNGSDIGGGASIMGTLSGGGK
                     EVITIGENCLIGANAGVGISLGDNCVVEAGCYLTGGSKITLPDGQVVKAKELSGASNL
                     MFWRNSVNGALEAKPKTGIWSGLNSILHVN"
BASE COUNT          381 a          330 c          371 g          456 t
ORIGIN      
        1 actgcaatgt cagcggctag ccattcaggt ttctgcgcgg caatttttgt caacccattg
       61 cgagcactgt ctacttcttc acattcataa aaaatatatg tgacatcaaa tactgggttg
      121 gtgagtgtta ccgcggcgcg cagcgcgaca gcaacaccgc ctttcatgtc gcaactacct
      181 aagccaaaga gcacatcttc agctagtgtg ctgccttcaa ctggaagctg ctgaccggct
      241 ttgagtaaaa cagctttatc attttctgct actggcacag tatctaagtg acctgccacg
      301 ataactcggc tggatctgcc taaatttgtt cgcgcgatca cattgttatt aattcgttca
      361 acggtaagcc agtcacatgc acgaagtgca ccttcgacta agtctgcaat ttgagcttca
      421 ttacccgaaa cacttggaat atcgatcaac tgagaagtta acgtgactac atcggcgtgc
      481 ggattaagca gtttttgggt cacacgacta cggtagtgct gatggacttt taatgtaaaa
      541 ctgcattctc ggtagtgttt gtttttaagg tttatttttg ggacgcaacg aaactgaaag
      601 tgggcaagat gaccaatttg tcggcatccg caattggcct agctacttat agtggcgaca
      661 cattacttga tgtttggtac ccaaatccga ctttgtccgc aacacctgcg aaagtttctg
      721 atttaactag ctccgcaaat tcagatgaat tgcgccaagt tcgctctgag gtcatcacta
      781 cagttgtctc agacctgagt accccgccta tagatgcagc cgatgtttac ttacggttac
      841 acctgctctc ccaccgcttg gtaaagcccc gcagtattaa tctcgatggc atctttggtc
      901 tgctgcaaaa tgttgcttgg acttcacttg gtgctgttgc gatcgacaaa ttaactgaag
      961 ttcagatttc agccagagct ggcggattat cgttcaatgt tttgggcgta gataagtttc
     1021 cacgcatggt cgattatgta gttcctagtg gagttcgtat tgctgacgct gatcgagtgc
     1081 gtcttggtgc ccatttagcc gaaggaacca ctgtgatgca tgaaggtttt gttaacttca
     1141 atgcgggcac acttgggacc agcatggttg aaggtcgcat ctcagctgga gttgttgttg
     1201 gcaatggctc agacattggt ggcggtgcca gcatcatggg aactctttct ggtggtggca
     1261 aagaggtcat cactatcggt gaaaattgtt taattggcgc aaatgctggt gttggtattt
     1321 cgcttggtga taactgtgtt gttgaagcag gttgctactt aactggtggt tcaaaaatta
     1381 cattgccaga tggtcaagtt gttaaagcca aagaattatc gggagcaagt aacttaatgt
     1441 tttggcgtaa ttcagttaat ggtgcgttgg aagcaaaacc aaagaccggg atctggtctg
     1501 gtttaaattc aattttgcac gtgaactaaa tttttagc
//
LOCUS       DBNHVN010000255         2221 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1533513, whole genome shotgun sequence.
ACCESSION   DBNHVN010000255 DBNHVN010000000
VERSION     DBNHVN010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1533513"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="AC16Y1_04845"
     CDS             complement(<1..699)
                     /locus_tag="AC16Y1_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7125 family protein"
                     /protein_id="HIR5831821.1"
                     /translation="MAKTPRASYKCTECGWTGPKWVGRCPECQAWSSVTEVSASPARA
                     GLRTTTTGTLPANPARAIKDIPVGEVERTSTGLAELDRVLGGGLVAGQCVLLAGEPGV
                     GKSTLLLEAADKFAKNGSKVLYISGEESAEQIRIRANRIGAKANTLLIADETDLAVLL
                     GHVEQVDPDLLVVDSVQTIASTELDGRAGGVAQVHEVTQVLTRAAKSRRMPLLLIGQS
                     TRDNSVAGPRSLEHL"
     gene            complement(732..1532)
                     /locus_tag="AC16Y1_04850"
     CDS             complement(732..1532)
                     /locus_tag="AC16Y1_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018157083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HIR5831822.1"
                     /translation="MPEKKLDFPRKWVEFIDPEDDTNLYRCDLTWLTSKWTCIWGAGC
                     KGIDKNNPDVGCCNLGAHIADKEDMDNTKKHAAMLRPDIWQNYSEAKKNGIFEKDDDG
                     DRKTRAINGACIFHNRNGFSGGDGCALHILSMEMGVGHHVTKPQVCWELPIRRTFENF
                     ERPDEVDTVIIGIEEYNRRGWGPGGTDLDWYCSTATEAHVGKQPVYISLEGELTSLMG
                     KKAYKILAEHCRAHEEMVAAVLKTGNEKTIKYLAIHPATRANQLKDKK"
     gene            1679..>2221
                     /locus_tag="AC16Y1_04855"
     CDS             1679..>2221
                     /locus_tag="AC16Y1_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013761.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HIR5831823.1"
                     /translation="MLRELLVSASRSKKLEHIVSTSPLSKSVVNRFVAGYDTSDVVRK
                     VSELHKLGIYTTIDRLGEDTLDRKQADSTVAAYLELLRALHAAGQTHMAEVSVKLSAV
                     GQALEPNGEEIALENARKIVEVANEVGTTVTFDMEDHTTIDSTLRILAEIRKSYPNTG
                     GVLQAQLHRTSNDVASNSYEG"
BASE COUNT          587 a          536 c          483 g          615 t
ORIGIN      
        1 taaatgttcc agagatcgtg gtccggcaac tgagttgtct cgagtggatt gaccaattaa
       61 aagtagtggc atccgtcgac tttttgctgc cctagttaat acttgagtta cttcatggac
      121 ttgggcaacg ccacctgctc gaccatctaa ttcagttgat gcgatagttt gcacactgtc
      181 tacgactagc aaatctggat caacttgttc gacgtggccg agtaaaactg ctaaatcagt
      241 ttcgtcggcg ataagtaatg tatttgcctt tgcgccaatc cgattcgctc taattcgtat
      301 ttgttctgcg gactcttcgc cagaaatata aagtaccttg cttccatttt tggcaaactt
      361 atcggctgcc tcaagaagca aagtagattt tccaacgccg ggctctccag caagtaacac
      421 gcattgccca gcaactaaac caccaccgag tacgcggtct aattcagcca aaccagttga
      481 ggtgcgctcg acttcgccaa ctggaatatc ttttattgct cttgctgggt tagctggcag
      541 tgtccccgta gtggttgtgc gcaggccggc ccgggcaggg cttgcgctta cttcagttac
      601 tgagctccag gcttgacatt cgggacaacg accaacccat ttaggtccgg tccaaccgca
      661 ttcagtgcac ttgtaactcg cacgaggtgt ttttgccacg ctctaagagt aagtgaaagt
      721 gccgacagtt gttatttttt atccttaagt tggtttgccc tagttgctgg gtgaatcgct
      781 aagtatttaa tagttttctc attgccagtt ttcaaaacag cagcaaccat ttcttcgtgg
      841 gcccggcaat gttcggccaa aatcttgtac gcctttttac ccatcagcga ggtcagctct
      901 ccctcaagtg aaatgtaaac cggttgcttg ccaacatgag cttcagttgc agtcgaacaa
      961 taccagtcaa gatcagttcc gcctggaccc caaccacggc gattgtattc ttcaataccg
     1021 atgatcacag tgtctacttc atcaggtcgt tcaaagtttt cgaatgttcg acgaattggt
     1081 aactcccaac aaacctgtgg cttagttacg tgatgtccaa ctcccatttc catggaaagt
     1141 atgtgaagcg cgcaaccatc gccacccgaa aaaccatttc gattgtgaaa aatacaggct
     1201 ccgtttatcg ctcgagtttt acgatcgcca tcatcatctt tttcaaaaat gccattcttt
     1261 tttgcttcgc tgtagttctg ccaaatatct gggcgcaaca tcgccgcatg ttttttggtg
     1321 ttatccatgt cttctttatc ggcgatatgt gcacctaagt tacaacaacc aacatctggg
     1381 ttatttttat cgatgccttt gcagcctgca ccccagatgc aagtccattt gctggttagc
     1441 caagtaaggt cacagcggta aagatttgtg tcatcttctg gatcaatgaa ctcaacccat
     1501 ttacgaggga aatctaattt tttctcaggc acgaaaagag cctaatcggt agctagcgaa
     1561 ataacccaac cgagtagacc tttttagaca gaggtagaac cgaaccacat tgcgaaacat
     1621 ttctcaggtg agcgtaaagt attcattagc caacgaagtc aattaggtag gacaccaaat
     1681 gctccgggaa ctgctagttt ctgcctcgcg cagcaaaaaa ctcgaacaca tcgtctcgac
     1741 ttcaccactg agtaagagtg tggttaatcg atttgtagct ggctatgaca cgagtgatgt
     1801 agttagaaaa gtttcagaac tccacaaact tgggatatac accactattg accgccttgg
     1861 cgaagacacc ttagatcgta agcaagccga ttcgacagtg gctgcttatc ttgagctact
     1921 tcgcgcattg catgcagcag gtcaaactca catggccgag gtatccgtca agttaagtgc
     1981 ggttggccaa gctcttgagc caaatggtga agaaattgcc ttagaaaatg cgcgcaaaat
     2041 tgttgaagtg gcaaatgaag ttggcaccac agttactttt gatatggaag atcacaccac
     2101 gatcgattca acacttagaa ttttggctga aattagaaag tcttatccaa ataccggtgg
     2161 agtgctacaa gcacagcttc atcgcacatc aaatgacgta gcaagcaatt cttatgaagg
     2221 a
//
LOCUS       DBNHVN010000256         5475 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_419874, whole genome shotgun sequence.
ACCESSION   DBNHVN010000256 DBNHVN010000000
VERSION     DBNHVN010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_419874"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1947
                     /gene="typA"
                     /locus_tag="AC16Y1_04860"
     CDS             85..1947
                     /gene="typA"
                     /locus_tag="AC16Y1_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019608173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR5831824.1"
                     /translation="MTRSAIRSDIRNVAIIAHVDHGKTTLVDAMLWQSGAFRANQDVN
                     ERVMDSMDLEREKGITILAKNTAVRYVGPAAPDGVTLNIIDTPGHADFGGEVERGLEM
                     VDGVILLVDASEGPLPQTRFVLRKALQKHLPVILVINKVDRPDARIAEVVDETYELFF
                     DLDATEAQIEFPIVYASAKAGRASLTRPVDGGMPDEENLEPLFRTLINTVPAPSYDPE
                     ATLQAHVTNLDSSPYLGRLALCRVHNGTIKKGQTVTWCKTDGSMEKAKVVELLITQAL
                     ERVPAESAGPGEIIAVAGFENITIGETLADAENPIPLPVITVDEPSISMTIGINTSPL
                     AGETGTKVTARLLKTRLDAELIGNVSIRVLETERPDTWEVQGRGELQLAVLVELMRRE
                     KYELTVGKPQVVTKQINGKLHEPIERLTIDVPEDYVGVVTQLLGLRKGRLEQMVNHGT
                     GWVRMEYLVPARGLIGFRTEFLTETRGTGLLHHIFEKYEPWVGELRTRPTGSLVSDRK
                     GAVTSYACFGIQERGTLFVSPGAEVYEGMIIGENSRQDDMDVNICKEKKLTNVRSATG
                     DELERLVPAKLLSLEQALEFCREDECVEVTPSAVRIRKVNLAKVDRDRVARKTR"
     gene            1954..3762
                     /locus_tag="AC16Y1_04865"
     CDS             1954..3762
                     /locus_tag="AC16Y1_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5831825.1"
                     /translation="MKAKLLALTKSVKFRRGSVAFLIAIFLLLVSTSSSQTINSASAF
                     NAGLDRAVGESTKAGGTLRIVSARDCNSLDPAASRDAWCSVILRLYTRNLLAHAGKAG
                     VAGLETVPDLAATSPEVTEENKVWTFTLRDNLTWDDGSPITSNDVKFSIERLYDDFLQ
                     SPVSNEILCLLSTCSSGSPDYLGPYGPDAAELATISTPDNKTVVFRLTRSFAQFDKIL
                     ATTNFGIISAKRDAELRASGNPYGQRPAASGPFKFVISEGIYKFIRNENWKQESDSIR
                     FPLVDEIQWTLSPDSETADIAVLNGEVDLRVDWGLGPAGRIQVLESEDQRKLLDNPKL
                     GYTNFLALIPNAPPLDRKACREAIAFALDKTALAATHGGTDVSVVANSMTPANILGYQ
                     ESFNPYPTGRQFTGDINKAREKLVECGYPDGFTVKFAFAQLGTGPQVYSVLQQSLGRV
                     GIVVDALPYDDFANYLMNGIGSPEVVKNTGIGLVAAGWSADYNSPISFWAPIVDSRKI
                     RIRSNQNIALLENQKVNQLLDDIEFSRTSDLAKLNQEIEKLIAADAVYLPISADSVLL
                     YRPANLANVYVQFALGSAYDLVNIGVNPQPEAETSP"
     gene            3862..4848
                     /locus_tag="AC16Y1_04870"
     CDS             3862..4848
                     /locus_tag="AC16Y1_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020131391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5831826.1"
                     /translation="MAGPTKLDSMESQSDQAQIVGRSLREIAWLRLKRDRVAMGSLFV
                     LLFIIFIAAFGPLISRWLGVDPYSFNSELISDNGGLPIGKFGGVSLDHPLGVEPLTGR
                     DILARLLYGSRISLLIAGSATLISVTLGTIVGIIAGNSRGRLDSILSRFMDITIAFPL
                     LLVIIAMSPVIEQRLQGLGLPEGNPSRITTLILVLSVFGWPYLARIVRGQVLSLRERE
                     FVEAAISIGSSNRRIIFKELLPNLWAPILVYTTIAMPSLIGAEATLAYLGISVVPPTP
                     TWGSMLEESVGYFTVDPFYFFVPLTMLLAAVLSFNLLGDALRDALDPKSGRT"
     gene            4941..>5475
                     /locus_tag="AC16Y1_04875"
     CDS             4941..>5475
                     /locus_tag="AC16Y1_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5831827.1"
                     /translation="MKKSTSIRFGVGVVSLALLLTACGGGSDDGASAGTKGGTLTFLT
                     EAEQILHLDPQRNYTGEDLAFASGYLNRTLTQYTLSADNNEASQLVADLATDTGTTPD
                     GGKTWEFTLKDGVKWEDGQAVTCEDVKYGVSRTFATDVITDGPTYAISMLDIAKDAEG
                     NSVYKGPYVEDAAGQAAY"
BASE COUNT         1545 a         1158 c         1263 g         1509 t
ORIGIN      
        1 attcaaattc catgcatcag caaacttaat gttcagtatt cactgcggta tttaactaat
       61 aaatactaag ttagactaag gacagtgacc cgatctgcaa ttagaagtga catacgcaat
      121 gtggcaatta ttgcccacgt agaccacgga aaaaccacct tagttgatgc catgctttgg
      181 caatctggtg ctttccgagc caatcaagat gtaaatgaac gcgtgatgga ttcaatggat
      241 cttgagcgtg aaaaaggcat tacgattctg gcgaaaaata ctgcagttag atatgtcggg
      301 ccagctgcgc cagatggtgt cacgctaaac attatcgaca ctccaggtca cgctgatttc
      361 ggtggtgaag tcgaacgcgg tctagaaatg gtcgacggcg ttattttgtt agtagacgca
      421 agcgaaggtc cacttcctca aactcgtttc gttttgcgca aagctttaca aaaacacctt
      481 ccagttattc tggtgataaa caaagttgat cggccagatg ctcgaattgc agaagttgtc
      541 gatgaaacct acgagttatt ctttgattta gatgccacgg aagcgcaaat cgaatttccc
      601 attgtttatg catctgccaa agctgggcgc gcctcactaa ctcgcccggt tgatggggga
      661 atgcctgatg aagaaaatct cgaaccactt ttccgcacgc tgataaatac tgtgccggca
      721 ccttcttatg acccagaagc aaccctgcag gcacatgtaa ccaacttgga ttcatcgcct
      781 tatttaggtc gcttagcgtt gtgccgggtt cacaacggaa ctattaaaaa gggccagaca
      841 gttacttggt gcaaaactga tggctcgatg gaaaaagcaa aagttgtcga acttctcatt
      901 acgcaagcgc ttgaacgagt cccagcagaa tctgccggtc ctggtgaaat tattgcggta
      961 gccggttttg aaaatatcac aattggcgag acgttagctg acgcagaaaa tccaatccca
     1021 cttccagtta ttacggttga tgagccgtca atttcgatga caatcggcat caacacttca
     1081 ccacttgccg gtgaaactgg cactaaagtt actgcgcgtc ttttaaaaac acgtttagac
     1141 gctgaattaa tcggaaatgt ttcaattcgt gttttagaaa cagaacgacc agatacctgg
     1201 gaagttcaag gccgcggcga attgcagtta gcagtgctag ttgaactaat gcgtcgagaa
     1261 aaatacgaac ttactgttgg taaaccgcaa gtagttacta aacaaattaa tggcaagtta
     1321 cacgagccaa ttgaacgctt aactatcgat gtgcccgaag attatgtagg tgtcgtaacg
     1381 caattgctgg gtctaagaaa aggccgacta gagcagatgg ttaaccatgg aactggttgg
     1441 gttcgtatgg aatatctggt tcccgctcgc ggcttaatcg gatttagaac cgagtttcta
     1501 acagaaacgc gcgggactgg gttacttcac catattttcg aaaagtacga gccatgggtt
     1561 ggcgaattgc gtactcgacc aactggctca ttagttagcg atcgaaaagg cgcagtaact
     1621 tcatatgcct gcttcggtat acaagaacgt ggcaccctat ttgtgtcccc gggtgctgaa
     1681 gtttatgaag gcatgatcat tggcgaaaac tcacgccaag atgacatgga tgtcaatatc
     1741 tgtaaagaaa agaaacttac taacgttcgt tcggcaacgg gtgacgaact agaaagacta
     1801 gtgccagcta aattacttag cctggaacaa gcactcgaat tctgtcgaga agacgaatgt
     1861 gtggaagtaa ctccaagtgc tgtgcgtatt cgcaaagtaa acttagccaa agtcgaccga
     1921 gatcgagttg ctcgaaaaac aaggtagtac tccgtgaaag cgaaattact tgcgttaacc
     1981 aagtcggtga aattccgccg agggtctgtc gcattcttaa ttgccatttt tttattgttg
     2041 gtttcaacgt cttcgtcgca aacaattaac tcagcctcag cttttaatgc tggtttagat
     2101 cgagcagtcg gtgaaagtac taaggctggt ggtactttgc gaattgtgag tgcccgcgac
     2161 tgtaactcgc ttgatccggc tgcaagtcgc gatgcttggt gttcagttat tttgcgttta
     2221 tacactcgta acctgttagc ccatgctgga aaagctggcg ttgccgggtt agaaactgtt
     2281 cctgatttag cggccacctc accagaggtg actgaggaaa ataaagtttg gacttttaca
     2341 cttcgcgata atttaacttg ggatgacggt tcacccatca ctagcaacga cgtgaagttt
     2401 tcaatcgagc gactttatga cgatttctta caaagtccag tatctaacga aatcttgtgt
     2461 ctgctatcga cctgttcctc aggctcccca gattatttgg gaccatatgg tccagatgct
     2521 gcggaactag cgactatcag cacgccggac aataaaaccg ttgttttccg attaactcga
     2581 agttttgctc aattcgacaa aattttagct acaactaatt ttggaattat ttctgcaaag
     2641 cgtgatgctg agctaagagc atcaggaaat ccttatggac aacgcccagc agcaagcggc
     2701 ccattcaaat tcgtaatttc cgaaggtatt tacaaattta ttcgtaatga aaattggaaa
     2761 caagagtcgg attccattcg ttttccgctt gtcgacgaaa ttcaatggac actttcaccc
     2821 gatagcgaaa cagcagacat cgctgtatta aacggagaag tcgatttgcg agttgactgg
     2881 ggtctaggtc ctgctggccg gatccaagta ttggaatcag aagatcagcg aaaattactt
     2941 gataatccaa aattgggtta caccaatttc ttggcactca tcccaaatgc accgccactt
     3001 gatcgtaaag cttgtcgaga agcaatcgct tttgccttag acaaaactgc acttgctgca
     3061 acccatggcg gaaccgatgt ttctgtagtg gcaaactcta tgactccggc aaacattctg
     3121 ggttatcaag aatcatttaa cccttatcca actggaaggc aattcactgg tgatatcaat
     3181 aaagccagag aaaaattagt tgagtgtggt tatccagatg gattcacagt taaattcgca
     3241 tttgctcagt tgggaacagg accacaagtt tactcagtac tccaacaaag tttgggtcgc
     3301 gtcggaatag tagtagatgc acttccatat gacgatttcg caaattattt gatgaatgga
     3361 attggttcac cagaagttgt taaaaatact ggcataggtt tagtggccgc tggatggtct
     3421 gcggattaca actcaccaat ttcattttgg gcgccaatag ttgatagtag aaagattcga
     3481 attcgaagta atcagaatat tgcgttactt gaaaatcaaa aagtgaatca acttcttgat
     3541 gacatagaat ttagtcgtac ttcagattta gccaagctaa accaagagat cgaaaaacta
     3601 atcgcagcgg atgcggtcta tttgcctatt tcagcagatt ctgtattgct ttatcggcca
     3661 gccaacctgg caaatgttta tgtgcaattt gcacttggat ccgcttatga tttggtcaat
     3721 attggggtaa acccacaacc agaggcagaa actagtcctt aatccagggg caatttccct
     3781 actttatata tgaaaaaatt atggcgaatt cagggtatgc aaatacacta aagaggctta
     3841 cttcgacaga aagttctcta aatggccggt cctacaaaac tagattcaat ggaatctcag
     3901 tcggatcagg cacaaattgt tggcagaagc ctaagagaaa ttgcgtggct aagacttaag
     3961 cgtgatcgag ttgccatggg cagcctattt gttttgcttt ttattatttt catagcagct
     4021 tttggaccat tgataagtcg gtggcttggt gtagatccgt attcatttaa ttcagagtta
     4081 attagtgata acggcggatt gccaattggc aaatttggtg gagtttcgtt agaccatcca
     4141 ttaggtgttg agccattaac aggtcgcgat attttggctc ggttactcta tggttcacgt
     4201 atctcattac ttatcgccgg tagcgcaact ttaatttcgg taactcttgg cacgatcgtc
     4261 ggaataattg caggtaattc cagaggtcga cttgactcaa ttttgagtcg cttcatggac
     4321 atcacaattg cttttccgtt gctcttggta atcattgcaa tgtcaccagt tatcgaacaa
     4381 cgtctacagg gtttgggttt accagaaggt aatccgtcgc gaattaccac actaattttg
     4441 gtgttgagtg tttttggctg gccatatcta gctcgaattg tccgtggtca ggttttgtca
     4501 cttcgggaac gagagtttgt cgaggccgct atttcaattg gctcgtccaa tcgtcgaatt
     4561 atctttaaag aattattacc gaacttatgg gcaccaattt tggtttatac aacgattgct
     4621 atgccaagtt tgattggcgc cgaagcaact ttggcatatt taggtatttc ggttgtacca
     4681 ccaacaccaa catggggctc gatgcttgaa gaatcagtcg ggtacttcac agttgacccg
     4741 ttctatttct ttgttccatt aactatgttg ttagccgcag ttttgtcgtt caatctattg
     4801 ggagatgcat tgcgagatgc acttgatcca aagagtggcc gtacataaaa acttcttgtg
     4861 acttcaaccg gagacataag aatgattcga gagaatcaaa aaaagttcga gagctcgaac
     4921 aaaaatatgg aggacaaacc atgaagaaat cgaccagcat ccgcttcggc gttggtgtgg
     4981 tgtcgttagc gcttctgtta accgcttgcg gtggcggaag tgacgacggt gcgtctgcag
     5041 gtacaaaggg tggaactctg actttcttaa cggaagcaga acaaattctt cacttagacc
     5101 cgcaacgtaa ctacacaggt gaggacctag catttgctag cggttacctc aaccgtacgc
     5161 ttactcagta caccctgagt gcggacaata atgaagcaag ccagctagta gctgaccttg
     5221 ctacagatac cggtactacc cctgatggtg gtaagacctg ggaattcacc ctaaaagatg
     5281 gcgttaagtg ggaagacggc caagccgtaa cctgcgaaga cgttaaatat ggtgtttctc
     5341 gtacattcgc aactgacgtc atcactgatg gcccaaccta cgcgatctca atgttggata
     5401 ttgccaaaga tgcagaaggt aattctgtat acaagggtcc atacgttgaa gatgcagctg
     5461 gccaagccgc atatg
//
LOCUS       DBNHVN010000257         1774 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_345123, whole genome shotgun sequence.
ACCESSION   DBNHVN010000257 DBNHVN010000000
VERSION     DBNHVN010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_345123"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..927)
                     /locus_tag="AC16Y1_04880"
                     /pseudo
     CDS             complement(1..927)
                     /locus_tag="AC16Y1_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391871.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
     gene            complement(928..>1774)
                     /locus_tag="AC16Y1_04885"
     CDS             complement(928..>1774)
                     /locus_tag="AC16Y1_04885"
                     /inference="COORDINATES: protein motif:HMM:NF025011.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HIR5831828.1"
                     /translation="DLTALAPGRLPAVARRAMAVLADVESTGVATTYRFTENSIRRAL
                     DQGQSAHEILDFLTNLSRTPLPQPLTYLIEDAAKRHGVLRVGVASIYLRCDDPQLVAT
                     VQADRRLASLKFRELAPGIVVSSSPADIVLDRLREAGYAPVAESAEGAVLIHRPDTKR
                     TSAKVPTPEVSISSVSDRLVAAAVKALRAGERADKAKPESGSGPKTTTAETLSLLNEA
                     LSKQLEVWIGYADKGGMTTERVIEPLSISGGFLSAFDVRSNEVRTFTIARITGVELMK
                     KEGSA"
BASE COUNT          456 a          437 c          405 g          476 t
ORIGIN      
        1 tctaaataca ggtgccggta acaggtggac ttcatcgtaa ataatcaagc cccagtcgcg
       61 ggcatcaaat aattccaagt gcgcataaac gccatttcgt tttgtcgtca taacttggta
      121 ggtcgcaatt gtcactgggc ggatctcttt gcgcgaacct gaatactcac caatttcttc
      181 ttcagttaag ttggtgcgct ttagtaattc tgatttccac tgccgagctg aaactgtatt
      241 tgtaacaaga attaaggtcg tcgcctttgc cgtggccatt gccgtagcgt caacaattgt
      301 tttacccgca ccacagggca acaccacaac accagaacca ccatgccaaa agccctctgc
      361 tgcgtccttt tggtactggc gcaggtcaca accctattgc tttaagtcga ttgggtgagc
      421 ttcaccatca acgtagccag ctaagtcttc ggctggccaa ccaagtttta gtaaggcctg
      481 cttaatcgtg ccgcgttcac ttgggtgaac tgcaactgtg tcatcgtcaa ttcgtttgcc
      541 aactaatgga gtaattttct tcgaccgaag tacttcttct aaaactgtgc ggtcatttgc
      601 ttgtaatacc aagccatgag ttggatgcat gtgtaaagat aagcgaccgt agcgagccat
      661 agtttcggca atgtcaacaa gcagggcatg tggtaccgga tagcgcgaat acttaagtag
      721 cgtgtcaaca acaaactctg catcgtggtt agctgcgcgc gcattccaca aacctaaatt
      781 ggtaattcga taggtatgaa tatgttctgg agctcgctct aattcagcaa atggtgcaat
      841 cgagtggcga cattcatcac ttaattcatg atcgatttca agcaataaag ttttatcgct
      901 ctgcacaatc aatgggccgt cagtcattta ggcactacct tcttttttca ttaactcaac
      961 cccagtaatt cgggcaatag tgaaggtgcg cacttcatta ctgcgcacat caaatgctga
     1021 taaaaatcca ccagaaatag aaagtggttc aatgactcgt tctgtcgtca tgccaccttt
     1081 gtcggcataa ccaatccaaa cttcaagctg tttgctaagt gcttcgttta gcagcgacag
     1141 cgtttccgca gtcgttgttt ttggcccact gccactttca ggctttgctt tatcagcgcg
     1201 ttcaccagcc cgaagggctt taactgcagc tgcgactaaa cgatcagata ccgaacttat
     1261 cgaaacttca ggcgttggga cttttgctga tgtgcgctta gtgtcaggtc gatgaattaa
     1321 aacggcacct tccgcacttt cggcaacggg tgcataacct gcttcacgta aacgatctaa
     1381 cacaatgtca gcaggtgatg aactaactac aattccaggg gctaattcac ggaacttaag
     1441 cgatgccaag cggcggtctg cctgcacagt tgcgaccaat tgtgggtcat cgcagcgtaa
     1501 gtaaattgag gcaactccca cgcgcagcac accatgtcgc tttgctgcat cttcaattaa
     1561 gtaggtgagc ggttgtggca gtggtgtgcg agacaagtta gttaagaaat ccaagatttc
     1621 gtgcgctgat tgtccttgat caagtgcccg acgaattgag ttttcggtaa accgataagt
     1681 agtcgctact ccggttgatt caacatcagc cagcactgcc atagctctgc gggctacagc
     1741 cggtagccga ccaggagcca gagcagttaa atcg
//
LOCUS       DBNHVN010000258         1328 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_50182, whole genome shotgun sequence.
ACCESSION   DBNHVN010000258 DBNHVN010000000
VERSION     DBNHVN010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_50182"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..687
                     /locus_tag="AC16Y1_04890"
     CDS             16..687
                     /locus_tag="AC16Y1_04890"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR5831829.1"
                     /translation="MWLAHLGVSVQLIAAIGDDLLGSWIRTKMTEGNLPTDQLQVVPT
                     QSSGTCLILVNSDGHRTMLPDPGANRSLELSTTQIQLIQNSSVVVMSAYTFLRPETRD
                     LANQVIEIVEKCSARLVLDAASSAPIAQVGVEKVREFLAKADLVLANEDEFELLAEAN
                     WSESLSNLIVKLGWQCPVFQAGSMYRHSLPLPKSFESVEFVRKLTQVKGWHRDQATLC
                     GGRQN"
     gene            complement(614..979)
                     /locus_tag="AC16Y1_04895"
                     /pseudo
     CDS             complement(614..979)
                     /locus_tag="AC16Y1_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
     gene            complement(976..>1328)
                     /locus_tag="AC16Y1_04900"
     CDS             complement(976..>1328)
                     /locus_tag="AC16Y1_04900"
                     /inference="COORDINATES: protein motif:HMM:NF016142.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HIR5831830.1"
                     /translation="FYRADSGFGVPWRADTPEEAALIGKRRDGIEAPGAVVIAKPVSA
                     KDELDLDLHDRVLKTGLAEAQKQGVVGKDVTPFLLDWFHTQTNNKSLEVNVTLINANA
                     KLAGQIAVAAAKQK"
BASE COUNT          347 a          292 c          310 g          379 t
ORIGIN      
        1 ccatgcaata tggcaatgtg gttggcccac ctaggtgtct cggttcagtt gattgccgca
       61 atcggtgatg atcttttagg ttcttggatt agaacgaaaa tgaccgaagg taacctgccg
      121 actgatcaac ttcaggttgt gccaactcag tcaagtggaa catgtctaat tttggttaac
      181 tcggatggcc accgcactat gttgccagat cccggcgcca accggtcact tgaattatct
      241 actactcaaa tccaactaat tcagaactct tcggtagtcg taatgagtgc gtacacattc
      301 ttacgtccag aaactcggga ccttgcaaat caagttattg agattgtcga aaagtgctcg
      361 gccagattgg tccttgatgc ggcttccagc gcgccgattg cccaagttgg ggtggaaaaa
      421 gttcgggagt ttttagctaa agcagatctg gttttggcaa atgaagacga atttgaatta
      481 ctagcagagg caaactggtc ggaaagttta agtaatttaa tagtaaaact tggttggcaa
      541 tgtcccgtgt ttcaggccgg aagtatgtat aggcactcat taccactacc gaagagtttt
      601 gaatcagttg aatttgtccg gaagttaact caagtgaaag gttggcaccg ggatcaggca
      661 acattgtgcg gtggccgtca gaattaacca aaattaagca agttccgctt gaatgcgttg
      721 gcactacctg aagttgatcg gttggtaagt caccttcggt cattttcgtt ctaatccaag
      781 aacctaaaag atcatcaccg attgcggcaa tcaactgaac cgagacacct aggtgggcca
      841 accacattgc catattgcat ggtgagccac caagacttaa cgaatttgtg gccttggtat
      901 ctgagttgat ttgaagcggc tcagctagca aactcacgat gtctacattg acatcaccga
      961 ttacggttac ttgattcatt tttgcttagc tgcggcaaca gcaatttgtc cagctaattt
     1021 tgcatttgca ttaattagcg tgacattgac ttcaagtgat ttgttatttg tctgagtgtg
     1081 gaaccagtcg agtaaaaatg gagttacatc tttgccaacg acaccttgtt tttgtgcttc
     1141 ggccaggcct gtttttaata ctcgatcatg taagtcaaga tcgagttcgt cttttgcaga
     1201 aactggcttt gcaattacga ccgcgcctgg tgcttcgatt ccatcccgtc gtttgccaat
     1261 taacgcagct tcttcaggtg tatctgcccg ccatgggaca ccaaaaccgc tatcagctcg
     1321 ataaaagc
//
LOCUS       DBNHVN010000259         2807 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_420278, whole genome shotgun sequence.
ACCESSION   DBNHVN010000259 DBNHVN010000000
VERSION     DBNHVN010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_420278"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="AC16Y1_04905"
     CDS             complement(<1..132)
                     /locus_tag="AC16Y1_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020101563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HIR5831831.1"
                     /translation="MSVASVYKLYLVKVERKNRTKKELDQVICWLTGLDQTELNKHLK
                     "
     gene            complement(157..1278)
                     /locus_tag="AC16Y1_04910"
     CDS             complement(157..1278)
                     /locus_tag="AC16Y1_04910"
                     /inference="COORDINATES: protein motif:HMM:NF017856.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HIR5831832.1"
                     /translation="MIKFSKIAVRKFFTPRNIAGEKVSFRDIINAPYFRLAVLSSLAG
                     TFAYFVGANTTFISGTTAAITTLVSVRHTFHDSIRESVTQVIGVLIGGTVAFVTIKFI
                     GFNSVVVLFAIFTCFVTARLLKLGEEGAIALGITLILVIGPRTESGALGTIETRFMGV
                     LLGATLAIVVSYFVRKGSPQSRALQAGLAQSLAMANLLNRISIKLALESETITRTMSV
                     AWLREAKKIVEELVEITNAAEGAVAGSQWSPIIDRAEATAILRQVQKTKITAETVVNI
                     CRELDLTFSNSEKLPETLAAALAGVLTATAHSILDQAEVAEDQPAAAIDVDEDGFEAQ
                     RQNTISELRHLDETQPLLIGGSILRDAEKINDLLTEEWK"
     gene            complement(1309..1734)
                     /locus_tag="AC16Y1_04915"
     CDS             complement(1309..1734)
                     /locus_tag="AC16Y1_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831833.1"
                     /translation="MSADLTNRIVVAVIERDETRIWTTNAEKGTKPEVIHQPAIADNH
                     KHVRDAQHHGGHESDKATKAYYESISKVLAPAGEILLVGHGNGKSNWVLKFVQHVERH
                     HKDVAQKIVGALDINVPALTENEILAAARDWFDEPIHRR"
     gene            1824..2711
                     /locus_tag="AC16Y1_04920"
     CDS             1824..2711
                     /locus_tag="AC16Y1_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5831834.1"
                     /translation="MTPRQLNMYAWVAIMWGSSFALLVPVVNSLGWAGGVSFRGFIAS
                     GIVLLVALATHRQLHFAGNWRHFAVLGATSVGMNLGGMNYALSHLGTQLTAILVTTIP
                     LYSLVIESIWTRKKPTLQKTVGLIIGFSGVVILIGLSPQAVNREFWFGVFGSFVASFG
                     FALGGNYSRAKAGHIGSWEQTIGTFLFGGILTLPLLLVAPIIRTPTVGDILCLFAVAA
                     TASSLAYILYFKLVAEVGATTALTTEYLVPVVAVILGYFLFSETLSATQILGSLVIML
                     GCALVMGFNPLTRKKALVK"
BASE COUNT          710 a          620 c          628 g          849 t
ORIGIN      
        1 tttcaaatgt ttattgagtt ctgtttgatc aagtccagtc aaccaacaaa taacttgatc
       61 taattccttt ttcgttctat tttttcgttc tactttgacc aaatacaact tgtaaacaga
      121 ggcaacactc atttggtaaa gcttgctcac ggttacttac ttccactctt cagtcagtag
      181 atcgttgatt ttttcagcat ctcgcaaaat agatccaccg atcagcagtg gctgcgtttc
      241 gtctaagtgg cgtaactcag aaatcgtgtt ttggcgttga gcttcaaacc catcttcgtc
      301 tacatcaatt gccgcagcag gttggtcttc agctacttct gcttgatcaa gtatcgaatg
      361 agccgtagca gttaacactc cagccagagc ggccgctaaa gtttcaggca acttttctga
      421 atttgaaaaa gttaaatcta attcacgaca gatattcacc acagtttcag cggtgatctt
      481 ggttttttgc acctggcgga gaattgccgt tgcttctgct ctgtcgatta ttggtgacca
      541 ttgacttccg gctaccgcac cttcagccgc atttgtgatt tcgactaatt cttcaacaat
      601 ctttttagct tctcgtagcc aggcaaccga cattgttctg gtgattgttt cagattctaa
      661 agcgagtttt attgaaattc gatttagcag atttgccatg gctaaagatt gggcaagtcc
      721 agcttgaagt gcgcgtgatt gtggcgaacc tttgcggaca aaatacgaaa cgacaatcgc
      781 aagtgttgct cctagtagga cacccataaa tcgagtttcg attgtgccaa gtgccccgga
      841 ctcggttctt ggaccaataa ccaaaatcaa agtaatacca agtgcgatag ctccctcttc
      901 gccgagcttt aataatcggg ccgtgacaaa gcaggtaaaa attgcaaata gaacaacgac
      961 cgagttaaag ccaatgaatt taattgttac aaatgcgaca gttcccccaa taagaacacc
     1021 aatgacttgg gtgacactct ctcggatcga atcatggaag gtatggcgaa ctgagaccaa
     1081 ggtagtgatt gctgcagttg tgccagagat aaaagtggta tttgcaccca caaaataggc
     1141 gaatgttcct gcaagtgaac ttaaaacggc taggcgaaaa tacggcgcat taataatgtc
     1201 ccgaaacgaa actttttcgc ccgcaatgtt tctaggcgtg aaaaacttac gaacggctat
     1261 cttcgaaaac ttaatcaggg tattgcctta cttttacttg ttcaagattt accggcggtg
     1321 aattggttca tcaaaccaat cacgagctgc ggctaaaatt tcattttcgg ttagggccgg
     1381 aacgtttata tccaaagcgc ccactatttt ctgtgcgaca tctttatgat ggcgctcaac
     1441 gtgttgaaca aatttaagta cccagttgga tttaccgttt ccgtgaccaa caagcaaaat
     1501 ttctcctgcc ggtgctaaaa ctttcgaaat agattcgtag taggccttag ttgccttatc
     1561 ggattcatgg ccgccgtgat gttgtgcatc acgaacatgt ttatgattat cggcaatcgc
     1621 cggctgatga attacttcag gctttgtccc tttttctgca ttcgtcgtcc aaatacgggt
     1681 ttcatcccgt tcaattaccg caacaacaat tcggttggtt aaatcagcac tcatcagaac
     1741 ctctttctct aggtcccagt ttaccgtttc aaataacctc cactgactca aaggaaattg
     1801 tcagtgcgcg atatagtcga actatgacac cacggcagct caacatgtat gcctgggttg
     1861 cgattatgtg gggctcatcc tttgctctac ttgttcctgt tgttaattcg ctcggttggg
     1921 cgggtggtgt ttcatttcga gggtttatcg cgagcgggat tgttctcttg gttgcattgg
     1981 caactcatcg ccagttgcat tttgctggaa attggagaca tttcgcagtt ttaggtgcaa
     2041 cgagcgttgg gatgaattta ggtggaatga actatgcgct atctcacctc ggcacacagt
     2101 tgaccgccat tttggtgacc acaattccgc tttacagttt ggtgatcgaa agtatttgga
     2161 cgagaaagaa gccaacactc caaaaaacag tgggcttgat tattggattt agtggcgtcg
     2221 taattctgat cggcctttca ccgcaggcag ttaatcgaga gttttggttt ggtgtttttg
     2281 gttcatttgt tgcatctttt ggctttgctt taggtggaaa ttattcgaga gccaaggctg
     2341 gacacattgg gtcgtgggaa caaacgatcg gcaccttttt attcggtgga attttgactc
     2401 tgccgcttct actagttgcg ccaattattc gcacccctac agtgggtgac attctttgtc
     2461 tattcgcagt ggcggccacg gctagttcat tggcttacat tttgtacttc aagttagttg
     2521 ccgaagttgg cgcgaccacg gctttgacga ctgagtattt ggttccagta gtcgctgtca
     2581 tactcggcta ctttttgttc agtgagacac tgtccgccac gcaaatactt gggtcgctgg
     2641 taattatgtt gggttgtgca ttagttatgg gatttaatcc attaactcga aagaaagcct
     2701 tagttaagta attaaatgac tgcatcgaaa tcaagtacag aagtcaagtt gcccaagcgg
     2761 tacggctgga ataccccagt gacagcacct ggtccaaaag gccgatc
//
LOCUS       DBNHVN010000260         1089 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_494344, whole genome shotgun sequence.
ACCESSION   DBNHVN010000260 DBNHVN010000000
VERSION     DBNHVN010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_494344"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="AC16Y1_04925"
     CDS             complement(<1..191)
                     /locus_tag="AC16Y1_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831835.1"
                     /translation="MAKRISLALTAILLVYLVFTVLRGLDFLAVDDPAVQALGVAVLV
                     LPFLGAILIYREIKFGKLS"
     gene            complement(207..938)
                     /gene="dapB"
                     /locus_tag="AC16Y1_04930"
     CDS             complement(207..938)
                     /gene="dapB"
                     /locus_tag="AC16Y1_04930"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR5831836.1"
                     /translation="MKVGVLGAKGRMGATVCSALSATSDLTLSCQLDAGDDISQLLIA
                     GVEVVVDFTTPHAVMANLEFLAKNNLHAVVGTTGFTDERIAQVQKLFSNSKANVLIAP
                     NFGLGAVLMMQFAAKAAPYFSSVEIVELHHPQKVDAPSGTAKRTAQMIASARQDSPKM
                     PDATADSLAGARGAVVDGIPVHSIRAAGLVAHQEVIFGGAGETLTIRHDSLDRESFMP
                     GVLLAVRNIPKTAGVTIGLEALLGL"
BASE COUNT          310 a          265 c          226 g          288 t
ORIGIN      
        1 taacttaatt tgccaaattt aatttctcga taaatcaaga ttgcacctaa aaacggcagc
       61 accagaaccg caactccaag tgcctgaacg gctggatcat cgacggctaa aaaatctagc
      121 ccgcgcaaaa ccgtaaaaac taaataaacc agcagtattg cggttaaggc aagtgaaata
      181 cgtttggcca tttattattt ttacttttac aagccaagta atgcttcaag gccgatggtg
      241 accccagcag tttttggaat atttctcact gcaagtagta caccaggcat aaatgattct
      301 cgatctagtg agtcgtggcg aattgttagc gtttctccgg caccaccaaa aataacttct
      361 tgatgggcaa ctaagccagc cgcccgaata ctatgtaccg gaatgccatc tacaacggct
      421 cccctggccc cggcaagtga atcggcagtg gcatctggca tttttggtga atcttgacgt
      481 gcgctagcaa tcatttgtgc agttcgctta gccgttccac taggtgcatc tactttttgc
      541 gggtgatgta attcgactat ttcgacgcta gagaaatatg gagcagcttt cgcggcgaat
      601 tgcatcatta aaactgcacc caacccaaaa tttggcgcaa ttagcacatt agcttttgaa
      661 ttactaaata acttttggac ttgtgcaatt cgctcgtcgg taaaaccggt agttccgaca
      721 accgcatgga gattattttt tgctaggaat tccaaattcg ccatgacggc atgaggcgtt
      781 gtgaaatcaa caaccacttc aacgcctgcg attagtaact gagatatatc atccccagca
      841 tctaactggc aacttaaagt taggtctgag gtggcagata aggctgaaca tacagtggct
      901 cccattcgtc cttttgcgcc gaggacacca actttgattg tcacttgttc accaccatgt
      961 ttgaaggtcc aaccaccgca gtcaacggat cttgtgacca caagtgatgt gccaacgaat
     1021 gtacttcatc aagcgtaact gccagaactc tatctagcac ctcactcacc gaagttaggt
     1081 agccataat
//
LOCUS       DBNHVN010000261         1579 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1608532, whole genome shotgun sequence.
ACCESSION   DBNHVN010000261 DBNHVN010000000
VERSION     DBNHVN010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1608532"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="AC16Y1_04935"
     CDS             complement(<1..937)
                     /locus_tag="AC16Y1_04935"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HIR5831837.1"
                     /translation="MSSPTVAELGEFALIAQIQKRLGVVDQTKVSVGIGDDSAVVSAA
                     GNQAVACVDVLSQGVHFRLDWSSANDIGRKAAAANLADIFAMGATPTALLVGLALPTH
                     TEVNWVLELADGLAQESSKVNTQVVGGDIVKSDVITISVTALGELNGRNPILRSGARP
                     GDTIAVAGKLGYSAAGLLMLSRGFRSPKVLVSAHRTPTPPYELAAKALAASSMIDVSD
                     GLISDLGHIAKASNVQINIDTKALQIPDELAATASAFNVNAFDWVLNGGEDHAFLATF
                     PTPLDVPNGWQIIGKVEVGDAKVLVDGQEQPNKGWD"
     gene            complement(984..>1579)
                     /locus_tag="AC16Y1_04940"
     CDS             complement(984..>1579)
                     /locus_tag="AC16Y1_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003806030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR5831838.1"
                     /translation="WKLNCEQTLARVKKLGFPLFVKPARAGSSVGISKVKVESDLIAA
                     IEFAREHDPKLIIEASMENTREIECGVLGGQNGPRASVLAEIIVREGHEFYDFEAKYV
                     DDSVELSVPAKLDPAISKRAQEIACAAFTAIDAEGLARVDMFITGEQIVINEINTMPG
                     FTPISMFPRMWDESGMSYPELIDYLIEDALRRGTGLR"
BASE COUNT          411 a          399 c          321 g          448 t
ORIGIN      
        1 ggtcccaacc cttatttggt tgttcctgcc catcaacgag aacttttgca tcgcccactt
       61 ctactttccc gattatctgc catccattcg gcacatcgag cggcgttgga aaagtggcta
      121 aaaatgcatg atcctcgcca ccatttaata cccagtcaaa ggcgttcaca ttaaaggcac
      181 ttgctgttgc tgctagttca tctgggattt gcaaagcctt ggtatctata tttatttgca
      241 catttgatgc tttcgctata tgaccaagat cagaaattaa tccatcgctt acatcaatca
      301 ttgaactggc cgctaatgct tttgcagcta actcgtaagg tggtgttgga gtgcgatggg
      361 cactaactaa aacttttggc gatctaaaac cccgggaaag cataagcaaa ccagctgctg
      421 agtaaccaag ttttccggca accgcaattg tgtcacctgg ccttgcgcca gagcgcaaaa
      481 ttggatttct accgttaagt tcgccaagtg cggtaacgct gattgtgatc acgtcacttt
      541 ttacaatgtc accgccgaca acttgggtat taactttgct actttcttgt gccagaccat
      601 cggcaagttc taaaacccaa ttaacttcgg tatgcgttgg aagtgctagt ccaaccaaaa
      661 gtgcagttgg agttgctccc attgcaaaaa tatctgcaag atttgcagcc gctgctttgc
      721 gcccgatgtc atttgcgctc gaccaatcga gtcgaaaatg tacaccttgg cttaatacat
      781 caacgcaggc tactgcttga tttccagcgg ctgagaccac cgcggaatca tcgccaattc
      841 ctacgctgac tttagtttga tcaaccacgc caaggcgttt ttggatttgc gctattaagg
      901 cgaattcgcc taattcggca acagtgggtg aactcaccgc aatccccatg gctcttcgat
      961 tcttcgatct tcgatctcag tcctcatcgc aatccagttc cgcgacgcaa agcatcctcg
     1021 attaaataat caattagctc gggatagctc attccagatt catcccacat ccgcggaaac
     1081 atagaaattg gagtaaatcc aggcatggta ttgatttcgt taataactat ttgttcgccg
     1141 gtgataaaca tgtctactcg agctaaacct tcagcatcga tggcagtaaa cgctgcgcaa
     1201 gctatttctt gggcgcgctt actaattgct ggatcaagtt ttgctggcac tgacaattcg
     1261 acagagtcat caacatattt agcttcgaag tcataaaact cgtgaccctc acgaacaatg
     1321 atttcggcca agacactggc gcgaggtcca ttctgaccgc caagaactcc acattcaatt
     1381 tcgcgagtat tttccatact tgcttcaata attaactttg gatcatgttc tcgagcaaat
     1441 tcaatcgcag ctattagatc actttcaact ttgacttttg aaatcccgac tgacgagccg
     1501 gcccgggcgg gtttaacaaa taatgggaaa cctagttttt tcacccgagc taaagtttgt
     1561 tcgcaattta gtttccagt
//
LOCUS       DBNHVN010000262         2122 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_2054739, whole genome shotgun sequence.
ACCESSION   DBNHVN010000262 DBNHVN010000000
VERSION     DBNHVN010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_2054739"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="AC16Y1_04945"
     CDS             <1..182
                     /locus_tag="AC16Y1_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="D-beta-D-heptose 1-phosphate
                     adenosyltransferase"
                     /protein_id="HIR5831839.1"
                     /translation="AMMRNSQEKVVLADSTKCDSIFPSVFSDGSDLDRIITDIDAPED
                     FVEGFGRQGVEVMLA"
     gene            283..1389
                     /locus_tag="AC16Y1_04950"
     CDS             283..1389
                     /locus_tag="AC16Y1_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020539903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIR5831840.1"
                     /translation="MSNFISSGAGNASGWRAAVQKLGGNLAGMVIPNIGAFIAWGLVT
                     ALFIPTGWIPNEELSKMVGPMILNLLPLLIAYTGGRMVHGHRGGVMGAVATMGVIVGT
                     DIPMFLGAMIMGPLAALVIKKIDAYLDPKSPVGFEMLISNFSLGISGTLMAILGFNAI
                     GPIVKTISEAAGSAVGTLVDNNLLPLAQLVIEPAKVLFLNNAINFGVLAPLGAIQAEE
                     TGKSILFMLETNPGPGLGLLLAFWVAGPKLLRGSIPSVSIIHFLGGIHEVYFPYVLMK
                     PRTLIGVILGGMAGVATNLALDNGLVATPSPGSVFAYMAVTPPGDQLKVLLAIAVSTA
                     VSFVACSFLLKTQKHAEDMGDLADAQSASRAMKG"
     gene            1396..1710
                     /locus_tag="AC16Y1_04955"
     CDS             1396..1710
                     /locus_tag="AC16Y1_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007540867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIB"
                     /protein_id="HIR5831841.1"
                     /translation="MSTVSGSDIKKVVVACEAGMGSSVMVAKQLAKQLKNHGVEVTHS
                     PVNQLAGEKPDVVLCHRGLQTRAKQAVPDTVVVAFDMFLGDPKIMSIVQAVESKGTLS
                     DD"
     gene            1703..>2122
                     /locus_tag="AC16Y1_04960"
     CDS             1703..>2122
                     /locus_tag="AC16Y1_04960"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HIR5831842.1"
                     /translation="MTSAPALTARGILLNRTASSREEAVRLCGQTLFDCGAIEQPYID
                     AMWERELLMSSFIGEATAIPHGTDASRKFVNFAQLVFLRFVNPIDWAGDAVSLAIGIA
                     SANDDHVDTLGVIAEILMDEHRKNILFEATNPQEIIEL"
BASE COUNT          566 a          445 c          514 g          597 t
ORIGIN      
        1 aagccatgat gagaaattca caggaaaaag ttgtgttagc tgacagcaca aaatgtgact
       61 ccatatttcc atcagtgttt tccgatggaa gtgacttaga tcgaatcatt accgacatcg
      121 atgcaccaga agattttgtt gaaggttttg gtcggcaagg tgtcgaggta atgctcgcgt
      181 aaagaaaaag gtttcttcaa tacgccgtat tggaggttcc aaatcagaaa aactttggtt
      241 gatctggtgc ggtaacaata aaaaaagatg aggaaaaact gaatgagtaa cttcatttca
      301 tcgggtgctg gcaatgcaag tggctggcgc gcggcagttc aaaaactcgg cggcaactta
      361 gccggtatgg tcataccgaa tattggtgcc ttcattgctt ggggtcttgt tactgcattg
      421 ttcattccaa ctggatggat tccgaacgag gaactatcca agatggttgg accaatgatt
      481 cttaacctct tgccgttact aatcgcttac actggcggtc gaatggtcca tggtcaccgc
      541 ggtggcgtta tgggtgctgt tgcgaccatg ggtgtaatcg ttggtactga catcccaatg
      601 ttcttgggtg caatgattat gggtccattg gctgcattag taatcaaaaa gatagacgct
      661 tatttagatc cgaagagccc agttggattt gagatgttaa tcagtaactt ctctttgggt
      721 atctccggaa ccttgatggc gattttaggt ttcaatgcaa ttggaccaat tgtaaaaact
      781 atctcagaag ctgccggtag tgcggtcgga actttagtag acaacaactt actgccactt
      841 gcgcaactag ttattgagcc tgcaaaagtt ctattcctaa acaacgccat taactttggt
      901 gtgctggctc cattgggtgc aattcaagcc gaagaaactg gaaagtcaat tctctttatg
      961 cttgaaacta accctggtcc aggccttggt ttgcttttag ctttctgggt tgctggtcct
     1021 aagttacttc gcggttcgat tcctagcgtt tcgattatcc acttcttagg tggtatccac
     1081 gaagtttatt tcccatacgt attgatgaaa ccaagaactc ttattggtgt aatccttggc
     1141 ggaatggccg gtgtagctac aaacctcgct ctagacaacg gtctggtagc aacaccatca
     1201 ccaggttcag tattcgctta catggcggtt actcctcccg gtgaccaact aaaagtccta
     1261 ctagccatcg cagtatcgac agcagtttca tttgttgctt gttccttcct actcaagaca
     1321 caaaaacacg ctgaagatat gggcgactta gcagatgctc agtcagcctc tcgggcaatg
     1381 aagggctaaa taacaatgag taccgtgtcg ggatcagaca ttaaaaaggt agttgtcgca
     1441 tgcgaagcgg gtatgggttc gagtgtgatg gttgccaagc agttagctaa gcaattgaaa
     1501 aatcatggcg ttgaggtaac tcatagccca gtaaatcaat tggccggtga aaagccagat
     1561 gtggtgcttt gtcaccgcgg tttacaaacc agagcaaagc aagctgtccc cgacacggta
     1621 gtcgttgcct tcgacatgtt cttgggcgat ccaaaaatca tgtcgatagt tcaggcggtg
     1681 gaatctaaag gcacactgtc tgatgactag tgccccagca ctcacggcaa gaggaatcct
     1741 tctgaatcgc acggcctcct cgcgagagga ggccgtgcgc ctctgtggcc aaacactttt
     1801 tgactgcggt gcaattgagc aaccctacat tgatgcaatg tgggaacgtg agctattaat
     1861 gtcttcattt attggtgaag caacagccat tcctcatggc acagatgcat ctagaaaatt
     1921 tgtaaatttt gcacaacttg tttttttaag gtttgtaaat ccaattgatt gggcgggtga
     1981 cgctgtttcg cttgcaatag gtattgcttc ggcaaatgat gatcatgttg atacgcttgg
     2041 agtcattgcc gagatactta tggatgaaca tcgaaagaat attttgtttg aagcaactaa
     2101 tccacaagaa attatcgaac ta
//
LOCUS       DBNHVN010000263         5483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1534281, whole genome shotgun sequence.
ACCESSION   DBNHVN010000263 DBNHVN010000000
VERSION     DBNHVN010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1534281"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="AC16Y1_04965"
     CDS             <1..798
                     /locus_tag="AC16Y1_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019181752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24B family metallopeptidase"
                     /protein_id="HIR5831843.1"
                     /translation="DQLRKANNFTVDGFIAVRKEIPQAIKAGGERWLQGTFDRHARTV
                     GQAPGYTSIVGSGAHAPILHWVRADGEVHPDELLLLDMGVEVRSGYTADVTRTIPASG
                     KFSSAQKQVHDLVEKSHRAGLAAVQPGRLWADFHAACMEVLAQGLHDWGMLPVSVDEA
                     LSENGQHHRRYIVCGVGHYLGLDVHDCDSSRYETYLGGKLEPGMALTVEPGLYFHAWD
                     ETVPPELRGIGIRIEDDLVVTDSGHEVISSALPIDAAGIEAWMASHE"
     gene            791..1201
                     /locus_tag="AC16Y1_04970"
     CDS             791..1201
                     /locus_tag="AC16Y1_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013125806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR5831844.1"
                     /translation="MNKKLEKSSRALAVLLVVTGLGHFIFPQPLDAIVPPALPFEPRF
                     WTYLSGVAELVVAGLLFVPAQVKFHGTSIKRIGIWSALALFILVYPANIYMAIDWSSR
                     PLAEALPAYLRLPFQFGLFYWCWQLLKLSKNVKN"
     gene            1341..2123
                     /locus_tag="AC16Y1_04975"
     CDS             1341..2123
                     /locus_tag="AC16Y1_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019852470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriorhodopsin-like"
                     /protein_id="HIR5831845.1"
                     /translation="MNNLSPLLTDNQYSTVYNMLSLGIASMLFTSLFLFVARDRVLPR
                     YRMAVMVSATVTAIAAYHYFRMFDSFKDAFTGETANIFAYNVGYRYVDWLLTVPLLLV
                     EVVAVLALARSVQRSLLNRLVPAATAMIILGYPGDARLDLFGLSATTWGILSTIPFLY
                     ILYVLFVELSKSLERQSEAVKKKVNTLRYLLIASWGVYPITFALGQNATGFDAAAFVN
                     REVGYTIADITAKCLFGLIIFTIARIKSAEDSREFAEAEYRD"
     gene            2211..3668
                     /locus_tag="AC16Y1_04980"
     CDS             2211..3668
                     /locus_tag="AC16Y1_04980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015794820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5831846.1"
                     /translation="MVDKLILVTGATGYIGGRLVRELVNGGKKVRVLVRDPAKIIGHS
                     WANEVEIAIGDAGSKEDLLKALKDVDVCYYLLHSLNVGPNFHEVENEMAMAFASAAKE
                     CQVKQIVYLGGINNDVAQSKHLSSRAQVGSVLAESGVPTIELRAGMVLGSGSASFEML
                     RHLTHRLPIMTTPKWINNRTHPIAVRDVLWYLKKCADLPSAQSGIFDIGGPDVLTYAE
                     LMQEFARVSKLAKRIIISIPVLTPTLASHWIGLVTPIPRSLAKNLVQSLISEVVADKN
                     KDISHLIPPPPGGTLPVKEAIELALQRVNDAQVDTRWSDANGPTSPWNKSATDPDWAG
                     ASVYRDKRELETDISVEKVWYQIESIGGETGWYGADILWSIRGLLDTIVGGAGLRRGR
                     RDPHKLRSGESLDFWRVEQIDHGRYLRLRAEMKLPGEAWLEFEVSHNGKTKIVQTASF
                     LPKGLFGHLYWYLILPFHFFVFPTMIRNIVKSARQ"
     gene            complement(3720..4487)
                     /gene="heR"
                     /locus_tag="AC16Y1_04985"
     CDS             complement(3720..4487)
                     /gene="heR"
                     /locus_tag="AC16Y1_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HIR5831847.1"
                     /translation="MTMKQITIPGLRRFNLIAGVVHLLQMIAVLALTNDFALPVNATY
                     MAGPPGSIYADPVTLFETPIGLSVALFLGLSALAHFIVISPKFFSRYSNGLGQKHNYF
                     RWVEYSISSSVMIVLIAQITGIADVTAIVAIFGVNASMILFGWLQEKYEQPGNGGWLP
                     FIFGCITGAVPWLAVLIYVLGPGNPSDAQAPAFVYGIVVTIFLLFNSFAWVQYKQYQA
                     KGKWANYLYGERIYIVLSLVAKSLLAWQIFANTLIPQ"
     gene            4544..5338
                     /locus_tag="AC16Y1_04990"
     CDS             4544..5338
                     /locus_tag="AC16Y1_04990"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR5831848.1"
                     /translation="MLFSRFVALGDSLTEGLSDRYPSGQFRGWADRVADQLSLQDQNF
                     KYANLAVRGKLVEEVVEQQLPKALELVNENTLISFHAGANNILRPSLDFDFVFEKYRT
                     AVERLSSTNAKVIVFTVREISRPQTPVEILWNRRFGPFNKNVHKVANEFGAKVMDANA
                     HDVFGAAQMLAADRLHLSAEGHRRVANAVLAELGYPHDNDWLDPFPATALAPLPLRFA
                     GNAIWAVGYVGPWLVRRVTGKSSGDGRTAKYPDLIEWQSKETFHKN"
     gene            5353..>5483
                     /locus_tag="AC16Y1_04995"
     CDS             5353..>5483
                     /locus_tag="AC16Y1_04995"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007537844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR5831849.1"
                     /translation="MSETLLTVYSTVWCGYCQRLKKQLAQQGINFVEIDIEENPEAA"
BASE COUNT         1502 a         1160 c         1301 g         1520 t
ORIGIN      
        1 gaccaattac gcaaagcaaa taatttcact gttgatggat ttattgcagt tcgaaaagaa
       61 attccacaag caataaaagc gggcggcgaa cgctggcttc aaggaacttt tgatcgtcat
      121 gcccgcacag ttggacaggc tccgggctac accagcatcg ttggttcagg tgcacatgcg
      181 ccaattctgc attgggtacg tgctgacgga gaagtccatc ctgatgaatt actgcttcta
      241 gacatgggcg ttgaagtgcg aagtggctac accgcggatg ttactcgcac aattccagca
      301 agtggaaaat ttagttcagc gcaaaaacaa gtacatgacc tagttgagaa atctcatcga
      361 gctggattgg ctgcagttca acctggccgg ttatgggcag atttccatgc tgcctgtatg
      421 gaagttctcg ctcaaggatt acatgactgg ggcatgcttc cagtttctgt ggacgaagca
      481 ctttccgaaa atggccaaca tcatcgtcgt tacattgttt gtggcgttgg acattacttg
      541 ggcttagatg tgcatgactg tgactcgtca aggtacgaaa cttatctggg cggaaagctt
      601 gagccaggga tggccttgac tgtagagcct ggtctttact tccatgcttg ggatgaaact
      661 gttccacccg agttacgcgg gattggtatt cgcatcgaag acgatctcgt tgttactgac
      721 tcgggccatg aagtgatttc ttcagcacta cctattgacg ctgcaggcat tgaagcctgg
      781 atggcttcac atgaataaaa aattagaaaa atcaagtcgg gcattagccg tgctgttagt
      841 tgtcacggga ttaggacact ttatttttcc gcagccgcta gatgcaattg ttccgcccgc
      901 tttacccttt gagccacgct tttggactta cttaagcggg gtagcagagt tggtggttgc
      961 cggtttacta ttcgtgccgg ctcaagttaa atttcatggc acttcaataa agcgaattgg
     1021 aatttggtca gccctggcat tattcatttt ggtttatcca gcgaatatct acatggccat
     1081 cgactggtct agtcggccat tagctgaggc acttccggcc tacttgcgat taccttttca
     1141 atttgggctg ttttattggt gctggcagtt actcaagtta agtaaaaatg ttaaaaatta
     1201 ggaatttgta cccttcctac aaaaaataac cccctttttg tgcgtgttcg agttgcaggg
     1261 gagcctccgt aggtggacgc taggagaagt ccggaggggc tcgtgggtgc acagcgcgtc
     1321 cacatttagg aaggaactaa atgaacaatc tctctccatt actgactgac aatcagtact
     1381 caactgtgta caacatgttg tcacttggta tagcgagcat gttattcaca tcgctgttct
     1441 tgtttgtcgc tcgtgatcga gttctacctc gttaccgaat ggcagtcatg gtcagtgcga
     1501 ctgtaactgc aatcgctgcg taccactatt tccgcatgtt tgactcgttc aaagatgctt
     1561 tcaccggtga aacagcaaat atttttgctt acaacgttgg ctaccgctac gttgactggt
     1621 tactaactgt gccgttgcta ctagttgaag tagttgccgt acttgcactt gctcgttcag
     1681 tacaacgctc actgctaaac cgtttggttc cagctgcaac tgccatgatc atccttggtt
     1741 acccaggaga tgctcgccta gatctattcg gtctaagtgc aacaacatgg ggcatccttt
     1801 caacaatccc attcctttac atcctttatg tattgttcgt tgaactctca aagagcttgg
     1861 aacgtcagtc agaggctgta aagaaaaaag taaacacact tcgctaccta cttattgcta
     1921 gctggggcgt ttacccaatc accttcgcgc taggtcaaaa tgcaactggc ttcgatgcag
     1981 cagcattcgt gaatcgtgaa gtcggctaca caattgcaga tatcactgca aagtgtctat
     2041 tcggcttaat cattttcaca atcgctcgaa tcaagtcagc tgaagatagc cgcgaatttg
     2101 cagaagcaga ataccgcgac taagaatcgc ctccataaca aacgagagtc acgaaagtgg
     2161 ctctcgtttg ttttatggca cagtccgggc aaatggcata cggttaaggc atggtcgata
     2221 aattaattct ggtgactgga gccactggct atatcggtgg gcgtttagtt cgagaattag
     2281 taaacggtgg caaaaaagtt cgagtcttag ttagggatcc agcaaaaata atcggccata
     2341 gttgggctaa tgaagttgag atagccattg gtgatgccgg ctccaaagaa gatctactta
     2401 aagcccttaa agatgttgat gtctgctatt acttattgca ctcacttaat gttggaccta
     2461 attttcatga agttgaaaat gaaatggcga tggcctttgc cagcgcagcc aaagaatgcc
     2521 aagttaaaca aattgtttat cttggtggca taaataatga tgttgcacaa agtaagcact
     2581 taagttcgcg tgcgcaggtt ggctcagttt tggctgaatc tggagtacca acaattgaac
     2641 ttagggctgg aatggtgttg ggttctggtt cggcgtcttt tgaaatgttg cgccatttaa
     2701 cacaccgcct accaatcatg accacaccta agtggattaa taataggacc catccaattg
     2761 ccgtgcgaga tgtactttgg tacttgaaga aatgcgcaga cttaccaagt gcacaatctg
     2821 gaattttcga catcggcggt ccagatgtac tgacttatgc agagttaatg caggaattcg
     2881 ctcgagtttc taaattagct aagcgaataa ttattagtat cccggtatta acgccaactt
     2941 tggctagtca ctggattgga ttagtcactc caattccacg atcccttgct aaaaatttag
     3001 ttcagtcact tatttcagaa gttgtcgccg ataaaaacaa agacatttct catttaatcc
     3061 cgcctccacc aggtggaacg cttccggtga aagaggcaat tgagttggct ttgcaaagag
     3121 ttaatgatgc gcaggtagat actcggtggt ctgatgcaaa tggtcccacc tcaccgtgga
     3181 ataaatcggc cactgatcct gattgggctg gagcttcggt ataccgagat aagcgagagc
     3241 tagaaactga tatttcggtc gaaaaagttt ggtatcaaat tgaatcaatc ggtggtgaaa
     3301 ctggctggta tggtgcagac attttgtggt ctattcgcgg tctactggac acaattgttg
     3361 gtggcgctgg cttgcggcgc ggtcgacgag atccacacaa gttacgcagt ggtgaaagtt
     3421 tagatttttg gcgcgttgaa cagatagatc atgggcgtta tttgcgatta cgggcagaaa
     3481 tgaaattacc tggtgaagct tggttggaat tcgaagtcag ccacaatggc aaaacaaaaa
     3541 ttgtgcaaac tgcttcattt ctgcccaaag gattatttgg acatctgtat tggtatctaa
     3601 ttttgccttt tcactttttt gtattcccta caatgattcg caacatcgtt aagtctgcaa
     3661 ggcaataaat aatcaaagcc cagtcagcaa gctaactggg ctttgatttt aggttagatt
     3721 tattgcggaa ttagtgtgtt cgcaaaaatt tgccatgcca acaatgattt agcaaccaaa
     3781 ctaagcacga tgtagatgcg ctcaccatat aaatagttag cccacttacc cttggcttgg
     3841 tattgcttgt attgcaccca ggcaaatgag ttgaataaca agaaaattgt tacgacgatt
     3901 ccgtatacga atgctggagc ttgagcatct gatgggttac cagggccaag tacgtaaatc
     3961 aatacagcta accaaggaac tgcgccagtt atgcagccaa aaataaatgg cagccaacca
     4021 ccgttacctg gctgttcgta tttttcttgt aaccaaccga acaggatcat cgaagcattt
     4081 acaccgaaga tagccacgat agcggtgaca tctgcgatac cagtgatctg agcaatcaac
     4141 acgatcatga ctgatgagct gatcgaatac tcaacccacc ggaagtagtt gtgcttttgt
     4201 ccaagaccat tgctgtaacg cgaaaagaat tttgggctaa tcacaataaa gtgtgcgagc
     4261 gcggacagcc ctaagaataa ggctactgat aaaccgattg gagtctcaaa aagtgtgacc
     4321 ggatctgcgt aaatcgatcc gggaggacca gccatgtatg ttgcattcac tggtagtgca
     4381 aagtcatttg ttagtgccaa aacggcaatc atttgtaata aatgaactac gccagcaatc
     4441 aaattgaagc ggcgtaatcc aggaatagtt atttgtttca tagtcataga gtaagtgtaa
     4501 ttagcctgat tcagacgttg aaatatgcag ttaggcttaa ttcgtgttgt tttcaaggtt
     4561 tgtagcgttg ggcgattcgc ttactgaagg actttctgac cgatatccaa gcggccaatt
     4621 tcgcggctgg gctgatcgtg ttgccgatca actttcattg caagatcaga acttcaaata
     4681 tgccaatttg gcagtgcgcg gaaaactagt tgaggaagtc gtcgagcaac aattgccaaa
     4741 ggcgttggaa ttagtcaacg aaaatacgct tatttcattc catgcgggcg cgaataacat
     4801 tttgcgacca agccttgact ttgattttgt ttttgaaaaa tatcgaactg cagttgagcg
     4861 actatcaagc accaacgcca aagtcatagt tttcacagtt cgagaaattt ctagacctca
     4921 gactccagtt gaaatattgt ggaataggcg atttggcccg tttaacaaga acgttcataa
     4981 agttgcgaat gaatttggcg caaaagtgat ggatgcaaat gcacatgacg tttttggcgc
     5041 agcacagatg cttgctgcgg atcgacttca tttgagtgct gaaggtcacc ggcgcgtagc
     5101 caacgcagtg ttggccgaac ttggatatcc acatgataac gattggctag atccatttcc
     5161 ggcgactgca ttggcgccat taccactgag atttgctgga aatgcaatct gggcggttgg
     5221 ttacgttggc ccgtggttag tgcgtcgagt caccggaaag tcatcgggtg atggtcgaac
     5281 agccaagtac cctgatttaa ttgagtggca atcaaaagaa acttttcata aaaattaacc
     5341 gaatgcgaaa tcatgtctga aactttatta actgtataca gcacagtctg gtgtggctat
     5401 tgccagcgac taaaaaagca actggctcaa caaggaatta acttcgtcga aatagacatt
     5461 gaagaaaatc cagaagctgc tca
//
LOCUS       DBNHVN010000264         5866 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_568928, whole genome shotgun sequence.
ACCESSION   DBNHVN010000264 DBNHVN010000000
VERSION     DBNHVN010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_568928"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /gene="obgE"
                     /locus_tag="AC16Y1_05000"
                     /gene_synonym="cgtA"
                     /gene_synonym="obg"
                     /gene_synonym="yhbZ"
     CDS             complement(<1..316)
                     /gene="obgE"
                     /locus_tag="AC16Y1_05000"
                     /gene_synonym="cgtA"
                     /gene_synonym="obg"
                     /gene_synonym="yhbZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016908787.1"
                     /note="ObgE; essential GTPase; exhibits high exchange rate
                     for GTP/GDP; associates with 50S ribosomal subunit;
                     involved in regulation of chromosomal replication; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR5831850.1"
                     /translation="MTTFVDRVVLHVQGGNGGNGCASVHREKFKPLGGPDGGNGGKGG
                     DIVLVVEPAVTTLLDFHRSPHRAAGNGKPGQGDHKNGAFGSDLELPVPEGTVVSLSDG
                     TVL"
     gene            complement(383..637)
                     /gene="rpmA"
                     /locus_tag="AC16Y1_05005"
     CDS             complement(383..637)
                     /gene="rpmA"
                     /locus_tag="AC16Y1_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR5831851.1"
                     /translation="MAHKKGASSTRNGRDSNAQRLGVKRFGGQLVNAGEIIIRQRGTH
                     FHPGLNVGRGSDDTLFALTAGAVEFGSRRGRRVINIVPAE"
     gene            complement(641..1195)
                     /gene="rplU"
                     /locus_tag="AC16Y1_05010"
     CDS             complement(641..1195)
                     /gene="rplU"
                     /locus_tag="AC16Y1_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR5831852.1"
                     /translation="MYAIVKAGGRQEKVAVGDLISLDRVAAKPGEVLTLPTVMIVDGG
                     KINAKAQSVVVSAEVIDHTKGKKIEILRYKNKTGYRRRQGHRSALTEVQIIAIGDVKI
                     SASDKVEAKTGKKLVQKTAKKTVAPVAKAPVAKEVSTESAEVKTEAVKKAPAKKAPAK
                     KAPAKKAPAKKAPAKKSAPKKTGK"
     gene            complement(1333..3297)
                     /locus_tag="AC16Y1_05015"
     CDS             complement(1333..3297)
                     /locus_tag="AC16Y1_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018511492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR5831853.1"
                     /translation="MSDEVTKPKRRAAGRPAGPPSIPAATDSSTPAEKKSAKKAPIVA
                     PVFQAAPVESVPVKKGAKKKDIKKPVAKNKTEEVEVKASEETSGTEDRGARRNRNRRG
                     GKNKNRPTRADRVEDENQTDDSTEEDSADDSTDSNSPGAHKRVRKRNRDGESDANSTR
                     IDAKRQRRRDGRDSGRRRAPILTEAEFLARRENVERVMVIRQQGSRTQIAVLEDDVLV
                     EHYVNQSSSGSLIGNVYSGRVQNVLPGMEAAFVDIGKGRNAVLYAGEVNWDAAGLDGQ
                     PKRIETALKTGDTVLVQVTKDPVGQKGARLTSQVSLPGRFLVYVPNNPMTGISRKLPD
                     RERVRLKNILKQVVPENGGVIVRTAAEGASEEELRSDVARLVAQWEDIEAKAKSAGPA
                     TMLYAEPDISIKVVRDIFNEDVNKLVVSGDDAWDTVDNYVRYVAPDLVDRIEKYVGTT
                     DIFTEYRIEEQLAKALDRKVYLPSGGSLVIDRTEAMTVVDVNTGKFIGQGGNLEQTVT
                     KNNLEAAEEIVRQLRLRDIGGIIVIDFIDMVLESNRDQVIRRLIECLGRDRTKHQVAE
                     VTSLGLVQMTRKRIGAGLLEVFSESCDHCNGRGVVINMDGTVAKTGRKAKGNSELNAD
                     QHQNGHHHDESDEKLAENGHIHQDDHETVS"
     gene            complement(3412..4140)
                     /locus_tag="AC16Y1_05020"
     CDS             complement(3412..4140)
                     /locus_tag="AC16Y1_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HIR5831854.1"
                     /translation="MARDPGREVIPAVQKLRIQHAKRGPLRFTSHRDFQRAFERALRR
                     ANVPMAYSSGFSPHPKISYANAAPTGVASEAEYVEIGVVEKCDPEKVQLALGAVLPPG
                     LDVIDVVQANTPDFVNRLEASHWQIELPGIGVEELAGAVAKFFDQTEIEVERMTKSGL
                     RKFDARNAVIHGVTESGQDCGILLIVVRHGTPAVRPDDVLAALTKVADLEIKIPPRIT
                     RLAQGPLLEDGWSVGDPLALDRNQ"
     gene            complement(4130..>5866)
                     /locus_tag="AC16Y1_05025"
     CDS             complement(4130..>5866)
                     /locus_tag="AC16Y1_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HIR5831855.1"
                     /translation="ALCERTYSVWPDLEALMREHKVPQFTVEAHRPVGAFDLFGLSFA
                     TELGYTNMLNAIDLAGVPIHSKDRTDEHPLVIAGGHAAFNPEPIANFIDAAVLGDGEQ
                     AVLAITDLVADFKKAGSPGGRNELLLRLAKSGIVYVPKFYDVEYLTDGRIRRVTVNES
                     GVPWRVAKHTVMNLDEWPYPKAPLVPIAESVHERMSVEIFRGCTRGCRFCQAGMITRP
                     VRERSKNTIADMVENGLKKTGYEEIGLLSLSSADHSEIAEISKGLADRYEDTQTALSL
                     PSTRVDAFNIDLANELSRNGRRSGLTFAPEGGSERIRRVINKQVSEEDLIRTVTAAFG
                     AGWRQVKLYFMCGLPTEQDEDVLQIAKLAHEVIKAGREASGRRDIRCTVSIGGFVPKP
                     HTPFQWAAQLDHETTDSRLYKLREAIRQDKEFGKSIGVRYHDGKPGIVEGLLSRGDRR
                     VGKIIEQVWKNGARFDGWSEHFSYEAWMQAAQEALADEPVDVNWYTIRERDLAEVLPW
                     DHLDSGLDKEWLWDDWQDSLSDVEVDDCRWTPCFDCGVCDHMGTEIQIGPTNGVILPL
                     PKVRSEVVVSGA"
BASE COUNT         1445 a         1496 c         1250 g         1675 t
ORIGIN      
        1 ccaatacggt tccatctgaa agtgaaacca ctgttccttc aggtactggt aattctaaat
       61 ctgaaccaaa agcaccattt ttatgatcgc cttggcctgg ttttccattt ccagctgcac
      121 gatgcgggct gcgatggaaa tccaaaagag tagtcacggc aggttcaacc actaaaacaa
      181 tgtcgccacc tttgccgcca ttgccgccat caggtccacc aagtggtttg aatttttctc
      241 gatgtactga agcgcaacca ttgccaccat tgccgccttg aacatgcaaa acaacacgat
      301 caacgaaagt tgtcatgttg tctccttcgt aataagaaaa gcgggcccag cgtgggcccg
      361 ctaatcaaaa acttattaaa agttattcgg caggcacgat attaattacg cgacggccac
      421 gacgtgaacc gaattcaaca gcacctgcag ttaacgcaaa tagtgtgtca tcgctaccac
      481 gacccacatt taatcctggg tgaaagtgtg ttccgcgttg ccgaatgata atttcaccag
      541 cgttaactaa ctgaccacca aaacgcttta cgccaagacg ctgtgcgttt gaatcgcgac
      601 cgttacgagt actcgaggca ccttttttat gagccatgac ttacttccct gtcttcttcg
      661 gggctgactt cttagccgga gcttttttcg ctggagcctt tttggctgga gctttcttag
      721 ctggagcttt cttagctgga gctttcttta cggcttcagt ttttacttct gcagactcag
      781 ttgaaacttc tttggcaacc ggagcttttg caaccggagc tacagtcttc tttgcagttt
      841 tttgaactaa cttcttgcct gtctttgctt caactttgtc gcttgctgaa atctttacgt
      901 caccaatagc aataatctga acttcggtaa gtgctgagcg gtgaccttgg cgacggcggt
      961 agccagtctt gtttttgtag cgaaggattt caatcttctt gccttttgta tgatcgataa
     1021 cttcagcaga aacaacaact gattgcgcct tggcgttaat ttttccgcca tcaacgatca
     1081 tgacggttgg aagcgttaaa acttcaccag gctttgccgc aacgcgatca aggctaatta
     1141 ggtcgccaac ggcaactttc tcttggcggc caccagcttt aacaatcgcg tacacgattt
     1201 ctccttgcac tcttttaagt gaactctttg ctttttcgtc agtttttagg tgaaaaccct
     1261 taatacgtaa gcctcataag gttacggatt aaagggcctt cgggtcaaac tgaagtcttt
     1321 gaattactcg acttaactga ctgtttcatg gtcatcttga tgaatatgcc cattttcagc
     1381 caatttttca tcggactcgt catggtggtg accattttgg tgctggtcag cgtttagctc
     1441 agaatttccc ttagctttgc gcccagtctt ggccacagtt ccatccatat ttatgacaac
     1501 accgcgacca ttgcagtggt cacaggattc gctgaatacc tcaagaagtc cggcaccaat
     1561 tcgctttcgg gtcatctgaa ctaatcccaa tgaagtaact tcggcaactt gatgtttggt
     1621 gcggtcacgc cctaaacact caattaatct gcggataact tgatcgcgat tactttcaag
     1681 caccatatcg ataaagtcaa tcacgataat tccgccaata tcgcgcaacc gtaattgccg
     1741 aacaatttct tcagcagctt ctagattgtt cttagttaca gtctgttcaa ggtttccacc
     1801 ctggccaata aatttgccag tgtttacgtc cactactgtc atcgcttctg tgcggtcaat
     1861 aactaatgaa ccacctgatg gtaggtacac cttacgatcg agagctttgg caagctgttc
     1921 ttcaatccgg tattccgtga aaatatctgt agtgcccaca tacttttcaa ttcgatctac
     1981 cagatctgga gcaacgtagc gaacatagtt atcgactgta tcccaggcat cgtcaccaga
     2041 aactacaagt ttgttaacgt cttcgttgaa aatgtcacga actaccttga tcgagatgtc
     2101 tggttctgcg tacaacattg ttgccggacc agcagatttg gctttcgctt caatgtcttc
     2161 ccattgagca actaaccttg ccacatcgct acgtaattct tcttcgcttg caccttcagc
     2221 agcagtgcgc acaattacgc caccattttc tggaaccact tgcttcaaaa tgttctttag
     2281 gcggacgcgt tcacgatcag gcaacttgcg cgatatgccc gtcatcggat tgttcggtac
     2341 ataaaccaag aaacgaccag gtagtgaaac ttggctagtt agtcgtgcgc ctttttgacc
     2401 gactggatct ttagttactt gaactaaaac agtgtcacct gtttttaatg cagtttcaat
     2461 tcgtttaggt tgaccatcga ggccggcagc atcccagtta acttctccgg cataaagaac
     2521 tgcgttacgt cctttaccaa tgtcaacgaa tgcagcttcc atgcctggta acacattttg
     2581 aactcggcct gaataaacat ttccaattag cgaaccacta ctagattggt ttacgtaatg
     2641 ctcaactaaa acgtcatctt cgagtactgc gatctgggtt cggctaccct gctgacgaat
     2701 caccatgact cgttccacat tttcccgacg tgctaagaat tcagcttcgg taagaattgg
     2761 cgcgcggcgg cgaccagaat cgcgaccatc acgtcggcgt tgacgtttgg catctatgcg
     2821 ggttgagttt gcatcagatt cgccatcacg gttccgtttg cgaactcgct tgtgtgcacc
     2881 tggggagtta ctatctgtcg aatcgtcagc tgaatcttcc tcagtggaat catcagtttg
     2941 gttttcgtct tcaacccgat cagctcgagt tgggcggttt ttattctttc caccacggcg
     3001 attgcgattg cgtcgagctc cgcgatcttc agtacctgaa gtttcttcgc tagcttttac
     3061 ttcgacctct tcagtcttat ttttcgcaac aggtttcttt atatctttct ttttggcacc
     3121 ctttttgact ggaacagatt caacaggtgc cgcctgaaaa accggtgcaa ctatcggtgc
     3181 tttttttgca gattttttct ctgctggggt gctcgaatca gtcgcagcag gaatacttgg
     3241 tggtccagct ggccgaccgg cagcacgacg ttttggtttt gttacttcgt ccgacatatg
     3301 tgtcgttctc ctatcgcact cacaaatgca tcttgtgcat tcggtgagcg atcacccgcg
     3361 ctccgagaag cgcttaagcc tatgtatgtg cggtgttcga gctacaaact gctattggtt
     3421 tcggtctaag gcaagtggat cgcctactga ccaaccatct tcaagcagtg ggccttgggc
     3481 taatcgagta atccgcggtg ggattttaat ttccaagtcc gcaactttag ttagagctgc
     3541 tagaacatcg tctggtcgaa cggctggcgt accatgccga acaactatta gaagtatccc
     3601 acaatcttgg cccgactcag ttaccccatg aattacggca tttctggcgt caaatttacg
     3661 tagtcccgac ttggtcattc gttcaacttc aatttctgtc tggtcaaaaa acttagccac
     3721 tgccccagct aattcttcaa ccccaattcc aggtaactca atttgccaat gactggcttc
     3781 gagtcgattt acaaaatctg gagtatttgc ctgcactacg tcaattacat cgagccctgg
     3841 tggcagtaca gcacctaatg cgagttgaac tttttctggg tcacattttt ctacgacacc
     3901 aatttcaaca tactcggcct cacttgcgac gccagttggt gcggcattgg cgtacgaaat
     3961 ttttggatgc ggcgagaaac ctgaactgta tgccatcggc acatttgctc ggcgtaatgc
     4021 gcgttcgaag gcccgctgga aatcgcgatg tgaagtaaac cgcagtggtc cacgtttggc
     4081 gtgctgaatt cttaattttt gaactgccgg gattacttcg cgccctggat cacgcgccac
     4141 taaccactac ctcagagcga acttttggta acggcagaat gacaccattt gttggaccga
     4201 tttgaatttc agttcccatg tgatcacaca caccacaatc aaaacatggg gtccatctgc
     4261 agtcatctac ttcaacatca gatagcgagt cttgccagtc atcccaaagc cattctttat
     4321 ctagccctga atctagatga tcccaaggta gaacttcggc taaatctcgt tcgcgaattg
     4381 tgtaccagtt aacgtctact ggctcatcgg caagcgcttc ctgtgctgcc tgcatccatg
     4441 cttcgtatga aaaatgctca ctccaaccat caaagcgtgc accatttttc caaacctgtt
     4501 caatgatttt tccaactcga cgatcaccgc gggatagtag gccttcgaca attccaggct
     4561 ttccatcgtg gtagcgaact ccaattgact taccaaactc tttatcctgg cgaatcgctt
     4621 cacgtaattt ataaagacgt gaatcagttg tttcatgatc gagttgagct gcccattgga
     4681 acggggtgtg cggtttaggc acaaaaccac cgatagatac tgtgcagcga atgtccctgc
     4741 gacctgaggc ttctcgccca gctttaatta cttcatgggc aagtttggct atctgtaaaa
     4801 catcttcgtc ttgttcggtg ggtagaccac acatgaagta caacttcact tggcgccaac
     4861 cagcaccgaa tgctgccgta acagttcgaa ttaaatcttc ttctgaaact tgcttattaa
     4921 taactctgcg gattctttca cttccaccct cgggagcaaa agttaaacca cttcggcgac
     4981 cattgcgaga aagttcattt gcaagatcga tattgaaggc atctacacgt gttgatggta
     5041 gtgataacgc agtctgggta tcttcgtaac gatcggccaa acctttagaa atttccgcaa
     5101 tttcactgtg atctgcactg gataacgaaa gtagtccaat ttcttcataa ccagttttct
     5161 tcaaaccatt ttcaaccatg tcagcgattg tgttttttga acgctcacgc accgggcgcg
     5221 taatcatgcc agcttggcag aagcgacagc cccgggtgca gccacggaaa atttcaacac
     5281 tcatacgttc atgcacgctt tcggcaattg gcacaagcgg agcttttgga tatggccact
     5341 catccagatt cataaccgta tgctttgcaa cgcgccatgg gacaccagac tcattaacag
     5401 tgactcgacg aatccgtcca tcagttaaat actcaacgtc ataaaacttt ggtacataaa
     5461 caattcccga ctttgctagt cggagtaaca attcattgcg accaccaggc gaaccagctt
     5521 ttttgaaatc agcaactaaa tccgtgatag caaggactgc ctgctcgcca tcaccaagaa
     5581 ctgcagcatc aatgaagttg gcaatcggtt ctggattaaa tgcagcatga ccgccagcaa
     5641 taaccaatgg atgttcgtcg gtgcgatcct tggagtgaat tgggacaccg gctaaatcaa
     5701 tggcattgag catgttggtg tagccaagtt cagttgcaaa tgaaagcccg aatagatcaa
     5761 atgccccaac tggccggtga gcttccactg taaattgggg aaccttgtgt tcgcgcatca
     5821 atgcttcaag atcgggccaa actgaataag tacgctcaca aagtgc
//
LOCUS       DBNHVN010000265         1882 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1832326, whole genome shotgun sequence.
ACCESSION   DBNHVN010000265 DBNHVN010000000
VERSION     DBNHVN010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1832326"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="AC16Y1_05030"
     CDS             <1..535
                     /locus_tag="AC16Y1_05030"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HIR5831856.1"
                     /translation="RLNPGADSNTYPDLANFARTLGAKIAVDASGPELKSAIEINPDL
                     IKPNQHELAELAGRPLLTIDEIVRAGNELIQSGVGSVLCSLGADGALFIDQNGVIHVE
                     PEQKVVGVPVGAGDILLATFIAGGANAEALPNAISWSAASVPLEGTAIPTPDQAAQVR
                     VRINNQLERSRSLVEVS"
     gene            536..973
                     /locus_tag="AC16Y1_05035"
     CDS             536..973
                     /locus_tag="AC16Y1_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR5831857.1"
                     /translation="MSVFAPELVEFNLSASDKTQAVMQMADLVVKSGRGNDAEQIVKD
                     VMARDEMGTPQVDGVAIPHARSSAVTKSSVVVARTAGVIFDPEEDPADVLFMILVPDG
                     ESNEHIEILSELARRLMDPEFTNILRTTSDANALVDLLSKGQE"
     gene            976..1284
                     /locus_tag="AC16Y1_05040"
     CDS             976..1284
                     /locus_tag="AC16Y1_05040"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: protein motif:HMM:NF051954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HIR5831858.1"
                     /translation="MKIVAITSCATGIAHTYMAAEALERAGKAAGHEIWVETQGAGGF
                     KPIDPTIIANADVVIFANDVGIREADRFSALKKVQSTPKTAIKDAKSLIEQAEKVVNE
                     "
     gene            1357..>1882
                     /locus_tag="AC16Y1_05045"
     CDS             1357..>1882
                     /locus_tag="AC16Y1_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HIR5831859.1"
                     /translation="MGAGTTVRQWLMTGVSYMIPFVAAGGILIAIGFMLAGSDNIVEV
                     TQPSLSEAVEANNIPALIFRLGAATFGFLVPVLSGYIAFAIADRPGLVPGFVAGAISG
                     AVGAGYLGGLAGGIVAGLVAKWISDQNVPSALRSVMPIVVTPLVATLVTGAVMVYIIG
                     EPVASLNQSLTNWLA"
BASE COUNT          508 a          391 c          469 g          514 t
ORIGIN      
        1 cagattaaat cctggcgctg attcaaatac atacccagac ttagccaatt ttgcccgcac
       61 cctaggagca aaaattgccg tagatgcatc tggccctgaa ctaaaaagcg caattgaaat
      121 caacccagat ttgattaaac ccaatcagca tgaattggct gaattagctg gtcgaccgtt
      181 actgacaatc gatgaaattg ttcgggcagg aaatgagcta attcaatccg gagttggttc
      241 tgtcctatgc agtttgggtg ccgatggcgc attatttatt gatcaaaacg gggttatcca
      301 cgttgaacca gaacaaaaag ttgtcggtgt gcctgttgga gctggcgaca ttttgctggc
      361 aacgtttatt gcaggagggg ctaacgccga agcattgccc aatgcgattt cgtggtcggc
      421 tgcctcagta cctttggaag gaacggcaat tccaactcca gatcaggccg cccaagtaag
      481 ggtgcgaatt aacaaccaac tcgaacgatc tagatctttg gtcgaggtga gttaaatgtc
      541 agtatttgcc cctgaacttg ttgagtttaa tttgtcggct agtgacaaaa cccaggcagt
      601 aatgcagatg gccgatttag ttgtgaaatc agggcgaggc aatgacgccg agcagatagt
      661 caaagatgtt atggctcgag atgaaatggg aactcctcaa gtggatggag ttgccattcc
      721 gcatgccaga agtagtgcag ttactaaatc aagcgtggtt gttgctagaa ctgctggtgt
      781 gatatttgat ccagaggaag acccagcaga tgtgcttttt atgattttgg ttccagatgg
      841 tgaaagtaat gaacacattg aaatactttc agagttagca agacggttaa tggatcccga
      901 gtttacaaac atcttgcgca ctacaagtga tgcaaatgca ctagtcgatt tacttagtaa
      961 aggtcaggaa tagaaatgaa aatcgttgca attacttctt gtgctactgg aattgcgcac
     1021 acttacatgg cagctgaagc acttgagcgc gctggtaaag ctgctggcca tgaaatttgg
     1081 gttgaaaccc aaggtgctgg tggtttcaaa ccgatagatc caacaatcat tgcaaatgcg
     1141 gatgtagtta tttttgccaa tgatgttggt atccgtgagg cagatcgatt ttctgcacta
     1201 aagaaagttc agtcaactcc aaagactgca attaaagatg ccaaatcttt aattgaacag
     1261 gctgagaaag ttgtgaacga ataataggtt tctgaagaaa atcttcagaa aaacacactt
     1321 gctacccgga agtgtgaaac gtaggaagga acagccgtgg gtgctggaac tacagtacgt
     1381 caatggttaa tgactggtgt cagctacatg atcccgttcg ttgccgctgg cggtattttg
     1441 atcgcaatcg gattcatgct tgcaggatcc gacaatatcg ttgaggtaac tcaaccttca
     1501 ctatcagaag cagtcgaagc caataacatt ccggctttga tcttccgcct gggtgcagcc
     1561 acatttggct tcttggtacc tgtgctttct ggttacatcg cattcgcaat tgctgaccgc
     1621 ccaggtttgg taccaggttt cgttgctggt gcaatctctg gtgcagttgg cgctggttac
     1681 cttggtggtt tagctggcgg tatcgtcgct ggtctagttg ctaagtggat ctcagatcag
     1741 aatgtgccat cagcacttcg cagtgtgatg ccaatcgttg ttactccact agtagcaacc
     1801 cttgttaccg gtgctgtaat ggtttacatc attggtgaac cagttgcttc gctaaaccaa
     1861 tcactaacta actggttagc tg
//
LOCUS       DBNHVN010000266         2279 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1832620, whole genome shotgun sequence.
ACCESSION   DBNHVN010000266 DBNHVN010000000
VERSION     DBNHVN010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1832620"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..112)
                     /locus_tag="AC16Y1_05050"
     CDS             complement(<1..112)
                     /locus_tag="AC16Y1_05050"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR5831860.1"
                     /translation="MSQVAVIGAGHGGLAVAARLAVKGHAVTVYESTDEVV"
     gene            complement(113..607)
                     /locus_tag="AC16Y1_05055"
     CDS             complement(113..607)
                     /locus_tag="AC16Y1_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019851802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HIR5831861.1"
                     /translation="MNSTADHPSVSRVKAFLAGHDIDVEVRHLSETARSAADAAAALG
                     VQVGQIASSIVFSIPEPLLIITSGRHRVDTELVASTAHLPKLGRADADFVKTWSGFVI
                     GGVSPVAWQHDGLEYIPQTFIDEALNEHDEIWAAAGHSHVVFRTNFQDLARISNATAI
                     FVGP"
     gene            complement(614..1939)
                     /gene="gabT"
                     /locus_tag="AC16Y1_05060"
     CDS             complement(614..1939)
                     /gene="gabT"
                     /locus_tag="AC16Y1_05060"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005479729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - GABA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HIR5831862.1"
                     /translation="MNEVPQERRLVTAIPGPKSQELQKRRTSAVSAGVGATLPIFVTK
                     AAGGVVVDIDGNSLIDMGSGIAVTSVGNSAKHVVDRVIAQVQDFTHTCFMVTQYEGYV
                     EVCEALNRLTPGDHEKRSALFNTGAEAVENAVKIARAYTKRQAVVVFEHGYHGRTNLT
                     LAMTAKVMPYKQGFGPFASEIYRMPLSYPAHDGLTGKEAADHAISRMIKEIGAENIAA
                     IVIEPIQGEGGFIVPAEGFLPGLVKFSNDNGIVFVADEVQTGFCRTGKWFACEHEGIV
                     PDLITTAKGIAGGLPLAAVTGRAEIMDAVHAGGLGGTYGGNPIACAAALGSIAAMEEE
                     DLLAKARNIEAVMKPRLAKIAEATGVIGDIRGRGAMMAIEIVKKGTNEPDPDTTSKIA
                     KACHAEGVVVLTAGTYGNVLRFLPPLVMPEHLLDDALTIIEQQIKEVTA"
     gene            2051..>2279
                     /locus_tag="AC16Y1_05065"
     CDS             2051..>2279
                     /locus_tag="AC16Y1_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5831863.1"
                     /translation="MTTAHPFWLAGKPATSGTLENVIHPGDGTLVGQYHVPNKDQVEQ
                     AVQAAWTVRQEYARTSAAFRAEALMNISRQLA"
BASE COUNT          608 a          565 c          512 g          594 t
ORIGIN      
        1 tcactacttc atcagttgat tcgtagaccg taaccgcatg accttttact gctaagcgcg
       61 cagcaacagc cagcccacca tgtccagcac cgatgactgc aacttgactc atttaaggac
      121 caacaaaaat tgccgttgca tttgaaattc gcgccagatc ttgaaagtta gttctaaaaa
      181 cgacgtgact gtgtccggca gcagcccaaa tttcgtcatg ctcgtttaga gcttcatcta
      241 taaatgtttg tggaatgtat tcaagtccat catgttgcca agcaactgga ctgactccgc
      301 caattacgaa tccactccag gtttttacaa aatctgcatc agcacgaccg agcttaggca
      361 aatgcgcggt gctggcaaca agttcagtat caactcgatg gcgaccagaa gtaatgatca
      421 gcaagggttc tggaattgaa aaaactatag atgacgcgat ctgaccaact tgaactccaa
      481 gtgctgctgc tgcgtccgca gcagagcgtg cagtttcaga taagtgacga acttcaacat
      541 caatgtcgtg gccagccaga aatgccttaa ctcggcttac cgaaggatgg tcggcagttg
      601 aattcataac ttattaagca gttacttcct tgatttgctg ctcaataatt gttaatgcgt
      661 cgtctagtaa gtgttctggc attacaagcg gaggcaagaa gcgcagaaca tttccgtaag
      721 ttcccgcagt taaaaccaca acaccttcag cgtggcaggc ttttgcaatt ttgctggttg
      781 tatcgggatc tggttcgtta gtgccctttt taacaatttc aatggccatc attgcaccgc
      841 gacctcggat gtctccgatg acgccggtag cttcagcaat ttttgctaac cgtggcttca
      901 tcacagcttc gatatttcgt gctttagcaa gtaaatcttc ttcttccatc gctgcaattg
      961 aaccaagtgc agcagcacaa gcgattgggt ttccgccgta agttccaccc agaccaccgg
     1021 catgaactgc gtccatgatt tcagcgcgac cagttaccgc agcaagtggt aaaccaccgg
     1081 caataccttt tgcagtggtg atcaaatctg gaacgatacc ttcgtgttcg caagcaaacc
     1141 atttgcctgt gcgacaaaat ccagtctgca cttcatctgc aacgaaaacg atgccattgt
     1201 catttgaaaa cttaactagt ccaggtaaga aaccttcagc tggaacaatg aagccacctt
     1261 caccttgaat tggttcaatc acaatcgcag cgatattttc tgcgccaatt tctttaatca
     1321 ttcggcttat cgcgtgatcc gcagcttctt ttccagttaa tccatcgtgt gcaggatagg
     1381 aaagtggcat gcgataaatt tctgaggcaa acggaccaaa accttgcttg taaggcatca
     1441 ctttcgcagt catcgcaagc gttaagtttg tacgaccgtg gtaaccgtgt tcaaagacga
     1501 ctactgcttg tcgttttgtg taagcacgag caattttgac tgcattttca acggcttcgg
     1561 cacccgtatt aaatagtgca gagcgcttct catgatcacc tggtgttaag cggtttagtg
     1621 cctcgcaaac ttctacataa ccctcgtatt gggtcaccat gaaacaggta tgggtgaaat
     1681 cttgaacctg agcaattacg cgatctacaa catgtttggc agagtttccg acgcttgtaa
     1741 cagcgatacc tgaacccata tcaataagag agttgccatc aatgtcgaca acaacgccac
     1801 ctgccgcttt cgtaacaaaa attggaagtg tggcgcccac gcctgcactc actgctgagg
     1861 tacggcgttt ttggagttct tgcgacttag gtcctggaat agccgtcact aagcggcgct
     1921 cttgcggaac ctcattaatt gtcattaccg aaatatcaga agcagtcata aaagtaatgt
     1981 tagacccaac tacaacaaag tagccatggc tggctttggc ttggtctgaa aaacggcgta
     2041 gcgtagggcc atgacgactg cacatccttt ttggctagcc ggcaaaccag ccactagcgg
     2101 aactctcgaa aatgtaattc acccaggtga tggcacttta gttgggcaat accacgtgcc
     2161 aaataaagat caggttgagc aagcggtcca agccgcttgg accgtgaggc aggagtatgc
     2221 ccggacgagt gcagcatttc gtgctgaagc actgatgaat atttcgcgtc agcttgctg
//
LOCUS       DBNHVN010000267         1877 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_866875, whole genome shotgun sequence.
ACCESSION   DBNHVN010000267 DBNHVN010000000
VERSION     DBNHVN010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_866875"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..91
                     /locus_tag="AC16Y1_05070"
     CDS             <1..91
                     /locus_tag="AC16Y1_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018848612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal-sulfur cluster biosynthetic enzyme"
                     /protein_id="HIR5831864.1"
                     /translation="LNWVWMPPWGPDKITDEGREMLRALGFNV"
     gene            116..1711
                     /locus_tag="AC16Y1_05075"
     CDS             116..1711
                     /locus_tag="AC16Y1_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR5831865.1"
                     /translation="MIQATGIELRAGADLLLANATFRIDAGDRVGLVGRNGAGKTTLT
                     KVLAGQALPAAGSVTSNGVGYLPQDPRTGDLDVVALDRILSARGLDELVRRMRVLEVE
                     MASTDETIRDRAMHKYTNAESAFQAAGGYAAESEAASLAASVGLTTNVLSQTLGTLSG
                     GQRRRIELARILFSDAQTLLLDEPTNHLDADSIAWLRTFLANHQGGLVVISHDVELLE
                     ATVNRVFHLDANRQALDIYNVGWKAYLTAREDDERRRKRERANAEKQASVLADQANRM
                     RAKASKAKAAQSMLKRAERLMDGLENERVADRVAKLRFPTPAPCGKTPLMASELSRSY
                     GSQEIFTDVDLAIDRGSRVVVLGLNGAGKTTLLRILAGVDKPDTGQIQPGHGLKIGYY
                     AQEHETLAPDRTVLENMRSASPDLTDTQMRSVLGSFLFSGDAVNKLASVLSGGEKTRL
                     SLAMLVVSAANVLLLDEPTNNLDPASRAEILDALAHFEGAVVLVTHDEGAVTALNPER
                     VLLLPDGVEDLWNTDYLDLIALA"
BASE COUNT          508 a          379 c          491 g          499 t
ORIGIN      
        1 attaaattgg gtttggatgc caccatgggg tccagataaa ataacggacg agggccgcga
       61 aatgcttcgc gctcttggtt ttaacgttta attaacccaa taggactggc gcacattgat
      121 tcaggcaact ggaattgagt tgcgcgccgg agccgatctg cttcttgcaa atgccacctt
      181 ccgcattgat gctggcgacc gagtcggttt agttggtcgt aatggtgctg ggaaaacgac
      241 tttaactaaa gtgcttgctg ggcaggcttt accggcagcc ggatccgtaa cttcaaatgg
      301 tgtcggatat ttaccgcagg atccgcgaac tggtgaccta gatgttgtgg cgttagatcg
      361 aatcctgagt gcacgtggct tagatgagtt agttaggaga atgcgggtac ttgaagttga
      421 aatggctagc accgacgaaa ctattcgcga ccgagctatg cataagtaca caaatgctga
      481 gagtgccttt caggcagctg gtgggtatgc cgctgaaagt gaagccgcaa gtcttgcggc
      541 cagtgttggg ttaacgacca acgttttaag tcaaacgctt ggcacgcttt ctggcggaca
      601 gcgacgtcga attgaattag ccagaattct atttagtgat gcccagactc tcttactcga
      661 cgaaccaaca aatcacctcg atgcggactc aatagcttgg ttgcgcactt tcttggcaaa
      721 tcatcaaggt ggtctagtgg ttatcagtca cgatgttgaa ttacttgaag caactgtaaa
      781 tcgggtattt cacttagacg ctaatcgcca ggctttagat atctacaacg ttggttggaa
      841 agcatattta actgctcgcg aagatgatga gcgtcggcgc aagcgcgagc gagcaaatgc
      901 ggaaaagcag gcgagcgtac tggccgatca agcaaatcgg atgcgagcaa aagcaagtaa
      961 agctaaagca gcccaaagta tgttgaaacg tgctgagcgt ttaatggacg gtttagaaaa
     1021 tgaacgagta gccgatcgag ttgctaaatt acgttttcca actccagcac cttgtggtaa
     1081 aacgccattg atggcaagtg aattgagtcg aagttatggt tcgcaagaaa ttttcacgga
     1141 tgttgattta gcaattgatc gtggtagtcg agtagttgtt cttggcttga acggtgctgg
     1201 caaaacgaca ctattacgca ttttggctgg ggtagacaaa cctgacactg gccaaattca
     1261 accgggacat ggtctaaaaa ttggttacta cgcacaggaa cacgaaactt tggcgccaga
     1321 tcgaacagtg ctagaaaata tgcgttccgc ttcacctgac ttaactgaca ctcaaatgcg
     1381 ttcggtactt ggttcatttt tattcagcgg ggatgcggtg aataaattag ctagcgtact
     1441 ttcgggtggt gaaaaaactc gattaagttt ggccatgttg gttgtatcgg ctgcaaatgt
     1501 tctgctttta gatgaaccaa ctaacaatct tgatccagcg agccgtgctg aaattcttga
     1561 tgcattggca cattttgaag gtgctgtagt gctcgtcacc cacgacgaag gggcggtaac
     1621 tgcattaaac ccagaacgcg tcttactttt acctgatggc gtggaagact tatggaatac
     1681 cgattattta gatttaattg cgttggctta acttatcgaa atgagtaatt aagtatgagc
     1741 atgccaggtg gaatgagtgc gttccatgca tatcgcgggt atacctctga taaatcagta
     1801 gttggacaac aacttgggcg acaaacgtta aaacgtattg gcggttacgc ggctgatttc
     1861 aaagtagaaa tattttt
//
LOCUS       DBNHVN010000268         1641 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_642453, whole genome shotgun sequence.
ACCESSION   DBNHVN010000268 DBNHVN010000000
VERSION     DBNHVN010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_642453"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="AC16Y1_05080"
     CDS             <1..557
                     /locus_tag="AC16Y1_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5831866.1"
                     /translation="RACWIAYVAQDPVMPQGMSVFNYVMLGRTAHLNMLAAESSKDID
                     IANYVIDEIGLHDFVDRDVATLSGGERQRVAIARALAQASPIVLLDEPTTALDVGYQQ
                     DVLELIDQLRKEKKIGVISTMHDLTVSGLYPDRLMLLANGKTVEEGSAEKVLTPETIG
                     KHYGAAVEVIASSNGPIVVPVRNN"
     gene            complement(572..>1641)
                     /gene="gcvT"
                     /locus_tag="AC16Y1_05085"
     CDS             complement(572..>1641)
                     /gene="gcvT"
                     /locus_tag="AC16Y1_05085"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012786050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR5831867.1"
                     /translation="HSKHLALGAKMGPFGGWDMPIEYTAGTVAEHNAVRTAVGIFDVS
                     HMGKVRILGEGAYEFANSVFANDLDRITPGQAQYSMYCNEAGGIIDDLIVYLVSKTEV
                     RFIPNAGNASTVVANLEKVVPSGLTIENNHLKQGILAVQGPKSSDVLKELGLPTEHDY
                     MAFVDATWQGHALTVCRTGYTGEHGFEIVISNDGLAPLFDALLAAGNKFGILPCGLGA
                     RDTLRTEMGYPLHGQDIAPDITPLEAKIGWAVGWKKEKFAGRDALVAQKESGAPRTAV
                     GLVANDRGIPRGHMDVKNSAGKVIGQTTSGTFSPTLQKGIALALVKPGVAEGDVLSVD
                     VRGRALSVTVTKPPFVAPSTK"
BASE COUNT          445 a          378 c          355 g          463 t
ORIGIN      
        1 ctagagcctg ttggattgct tacgttgctc aagatccagt catgccacag gggatgtccg
       61 ttttcaatta cgtaatgctt ggacgcaccg cacacttaaa tatgctcgcc gctgaatcca
      121 gtaaagacat tgatatcgcc aactacgtta ttgatgaaat tggattacat gattttgtcg
      181 atcgcgatgt ggctacgctt tcgggtggtg aacgtcaacg ggtggcaatt gctagagctc
      241 tagcccaagc cagtccaata gttctgctcg atgaaccaac tactgctttg gatgtgggct
      301 atcaacaaga cgtgcttgaa ctaattgatc aactacgcaa agagaagaaa attggtgtga
      361 tctcgacgat gcacgactta actgtttctg gactctatcc agatcgactt atgttgctgg
      421 ccaatggtaa aacagtcgaa gaaggcagtg cagaaaaagt attaacgcct gaaacaattg
      481 gcaaacacta tggcgccgca gttgaggtaa tagcgagctc aaatggtccg atagtcgtgc
      541 cagttcggaa taactaaata cttatcgaaa gttatttagt acttggtgca acaaatggtg
      601 gtttggtaac cgttacagaa agtgcgcgac ctcgaacatc tacagatagc acgtcgcctt
      661 cagcaactcc aggtttaact agggctagtg caataccttt ttgtagtgtt ggtgaaaaag
      721 taccgcttgt tgtttgacca attaccttgc cggctgaatt tttaacatcc atatgcccac
      781 gtggaatgcc gcgatcgtta gcaactaatc caactgcagt tctcggtgca ccagattctt
      841 tctgtgcaac tagcgcatcg cgaccagcaa atttctcttt cttccagcca actgcccaac
      901 caatttttgc ctctagtgga gtaatgtctg gcgcaatatc ttgaccatgt aatgggtatc
      961 ccatttcagt gcgcaatgta tctcttgcac ccaagccaca tggcagaatc ccaaatttgt
     1021 ttccggcagc aagtagcgcg tcaaataacg gtgctaaccc atcattgcta attacgattt
     1081 caaacccatg ctcaccggtg tagccagttc ggcaaacagt taaagcatgt ccttgccagg
     1141 ttgcatctac aaatgccatg taatcatgtt cggtaggtaa acctaattct tttagtacgt
     1201 ctgaagattt tggaccttga actgcaagaa ttccctgttt taagtgattg ttttcaattg
     1261 ttaagccact cggtacaact ttttctaagt ttgcaacgac tgtcgaagcg tttccagcat
     1321 tagggataaa tctaacttca gttttgctca ctagataaac aatgaggtcg tcgataattc
     1381 caccagcttc attgcaatac atcgagtact gagcttgtcc cggagttatt cggtctaaat
     1441 cattggcaaa aacagagtta gcaaattcgt atgcgccttc tcccaatatt cgaactttac
     1501 ccatgtgcga aacgtcaaaa attccaactg cagttcgaac tgcgttatgt tcggcaacag
     1561 ttccggcggt gtattcgatt ggcatatccc aaccaccgaa tggacccatc ttggcgccaa
     1621 gggctaggtg ttttgaatgt a
//
LOCUS       DBNHVN010000269         2456 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1164358, whole genome shotgun sequence.
ACCESSION   DBNHVN010000269 DBNHVN010000000
VERSION     DBNHVN010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1164358"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="AC16Y1_05090"
     CDS             <1..614
                     /locus_tag="AC16Y1_05090"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR5831868.1"
                     /translation="HASLAKRMNCDGLMLAGPAYSVPTEDELAAHLLTVVNAVKMPTV
                     LYDYPARIGVSVTQNVLNQVADNEYVIGIKEASGDLSRIPMLQQNFAGRVEPVCGADV
                     DSIVFMEAGVKSWIAGIANALPKAHLGIMDPNTRMAAHFAIKPLFEYIESGQYINKTK
                     ALMGILGVPANFLRGPLKSISAEQTNKLCELVDAAGEWAPKLV"
     gene            complement(660..1022)
                     /locus_tag="AC16Y1_05095"
     CDS             complement(660..1022)
                     /locus_tag="AC16Y1_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR5831869.1"
                     /translation="MEILEIVGRVMFALLFIGSAFGHFKNTAGMTAYAQSKGVPAPKL
                     AVQGSGLLILVGAVFIATGFQAALGALLLVIFLVPTAVLMHPFWKETDQMTKMNEQIS
                     FLKDISLAGAALVMFTLL"
     gene            1143..1778
                     /locus_tag="AC16Y1_05100"
     CDS             1143..1778
                     /locus_tag="AC16Y1_05100"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF003588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR5831870.1"
                     /translation="MWDRLSNEWQTELKHLKPKIEIISSRLRVEPQINPQEDLIFRAL
                     GKSPNDFRVVIVGQDPYPNPDLAMGLAFSIPDNQSMPPSLRNIKKELANDLGVSIGND
                     LSSWTHQGVLLLNRILTCRTGQSLSHKDFGWQEITSEIIEVIHRINPNVVGILWGKFA
                     QELSDYFNPNLLIKSAHPSGLSAYRGFFGSKPFSQANELLRRTSQKPVEWG"
     gene            1786..2232
                     /locus_tag="AC16Y1_05105"
     CDS             1786..2232
                     /locus_tag="AC16Y1_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5831871.1"
                     /translation="MATPTSVLLVELVMSVDRLIQSAKSATTRAGQWAPQIVLGHVSQ
                     VDTQVWLPRMQQMVTALENGEQEPSFVWWEPDPVQTEMLYKDLSLDDVSALVLQSRTN
                     IVTYLRELTPQQWQARAIHSTFGSIDVSELVFQALTHDEEHRASFV"
     gene            2376..>2456
                     /locus_tag="AC16Y1_05110"
     CDS             2376..>2456
                     /locus_tag="AC16Y1_05110"
                     /inference="COORDINATES: protein motif:HMM:NF015944.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR5831872.1"
                     /translation="MVDESFGQIEVQLQRRADLIPNLVETV"
BASE COUNT          750 a          497 c          535 g          674 t
ORIGIN      
        1 accatgcctc acttgcaaaa cggatgaatt gcgatgggtt gatgctggct gggccggcat
       61 acagtgttcc aactgaagat gaacttgccg ctcacttact taccgttgtg aacgcagtaa
      121 aaatgccaac tgtactttat gactatccag ctcgcattgg ggtcagtgtc acgcaaaatg
      181 tactgaatca ggttgcggac aatgaatacg taatcggcat taaagaagct agtggtgatc
      241 taagcagaat cccgatgctg caacaaaatt tcgccggtcg ggtagagcct gtttgtggcg
      301 cagatgttga ctcaattgtc ttcatggaag ctggagttaa gtcatggatt gctggcatcg
      361 caaatgcgtt gcccaaagca catcttggaa ttatggatcc taatactcga atggcagcac
      421 atttcgccat taagccatta tttgaataca tcgaatctgg tcaatacata aataagacca
      481 aggcgttgat gggaatactt ggcgtcccag caaacttcct acgtggaccg ctgaagtcaa
      541 ttagcgcaga acaaacaaat aaactttgcg agttagttga tgccgccggg gaatgggcgc
      601 caaagctagt ctgaaaataa aaatgggcgg cttggccgcc catttttatt taataacttt
      661 taaagcagag tgaacattac taatgctgca ccagcaagcg aaatgtcctt taagaaacta
      721 atttgttcgt tcattttcgt catttggtca gtttctttcc agaatgggtg cataagaact
      781 gcagttggaa ccaagaaaat cactagaagc agtgcgccta gtgcggcttg aaaaccagtt
      841 gcaataaata ctgcgccaac cagaatcaat aatccggaac cttgtacagc tagttttgga
      901 gctggtacac ctttagattg tgcgtaagct gtcatgccgg cagtgttctt aaaatggcca
      961 aatgctgaac caatgaataa taaggcgaac ataactcgac ctacgatttc taaaatttcc
     1021 acaaaaactc ctatttgtaa ctagtggaac taactacaac aagttagcag atagttgaat
     1081 tttcatttat cacttgggtg cgtgtcggaa gcgaattaac cagatatcct taagcggtgg
     1141 aaatgtggga tcgcctttct aacgaatggc aaacagaact caaacattta aaaccaaaaa
     1201 ttgaaattat ttcatccaga cttcgtgtag aaccgcaaat taatccacaa gaagatctga
     1261 tttttcgagc gttaggaaaa agcccaaacg attttcgagt cgtaatcgtt gggcaagatc
     1321 cctatccaaa tccggatttg gcaatgggtt tagcattttc aattccagac aatcagagta
     1381 tgccgcccag tctgcgtaac attaaaaaag agttagcgaa tgatcttggc gttagcatcg
     1441 gcaatgactt aagcagttgg actcaccaag gagtacttct cttgaatcgg attttaactt
     1501 gtcgaaccgg ccagtcactt tcacacaaag actttggctg gcaagaaatc acttcagaaa
     1561 ttattgaagt gatccatcga attaatccaa acgtagttgg aatactctgg ggaaaattcg
     1621 cccaagagtt aagtgattat ttcaatccaa acctgctaat taaatctgcg catccttctg
     1681 gattgagtgc atatcgagga ttctttggat caaagccatt tagtcaagca aatgaattat
     1741 tgcgtcgaac ttcgcaaaaa ccggtcgagt ggggataatt tacaggtggc tactccaact
     1801 tcagttttat tagttgaact tgttatgagt gttgatcgat taattcaatc agcaaaatcg
     1861 gcaaccacga gagcagggca gtgggcacct cagattgtgc tcggacatgt atcgcaggta
     1921 gatacgcaag tttggttgcc tagaatgcaa caaatggtca cagcccttga aaatggagaa
     1981 caagaaccat cttttgtttg gtgggaacca gatccagtgc agaccgaaat gctctataaa
     2041 gacttgtcgc tagatgatgt gagtgcattg gtgctccaaa gtcggacaaa tattgtcacc
     2101 tatctaagag agctaactcc ccagcaatgg caagcacgag ccattcattc aacttttggt
     2161 tcaatcgatg taagtgaatt agtctttcaa gctctgacac atgatgagga acaccgggca
     2221 agttttgtct aaagcgaaca aggttttttc agcagactaa attttggaat gtcaagtaaa
     2281 atcgaagtat ggagattact tacttactta ttgcactcgc tcttatagtc atttttttga
     2341 ttattcaata caaccgttta atcaggctaa atgtcatggt ggatgagtca tttggtcaaa
     2401 ttgaagttca acttcaacgc cgcgcggatc ttattccaaa tctagttgaa actgta
//
LOCUS       DBNHVN010000270         3660 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_793491, whole genome shotgun sequence.
ACCESSION   DBNHVN010000270 DBNHVN010000000
VERSION     DBNHVN010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_793491"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="AC16Y1_05115"
     CDS             complement(<1..351)
                     /locus_tag="AC16Y1_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012296630.1"
                     /note="catalyzes the formation of
                     2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at
                     position 37 in tRNAs that read codons beginning with
                     uridine from N6-(dimethylallyl)adenosine (i(6)A); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR5831873.1"
                     /translation="MTSTLESNALGTYEVRTMGCQMNVHDSERFAGLLEAAGYEPAPE
                     GELADVIVFNTCAVRENADNKLYGALSHLQPIKLKNKNLKIAVGGCLAQKDRGEILKK
                     APYVDVVFGTHNIDA"
     gene            complement(361..912)
                     /locus_tag="AC16Y1_05120"
     CDS             complement(361..912)
                     /locus_tag="AC16Y1_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIR5831874.1"
                     /translation="MFQMAASGSDPLDLKENPEADPLSVAKNIALRQLSLAPKTRFQL
                     EQVLAKKGVDPEVAKASLDRLTEVGLIDDLAYAGMFVRSKCVAKKISRSVLRMEMRQK
                     GISDEHIEQALSQVTDEDEYRMATELVVKKLRSMRSLEKNVIERRLYGLLARKGYNSG
                     VVIRVIKEQLSEFSADQDLADFA"
     gene            complement(931..2049)
                     /gene="recA"
                     /locus_tag="AC16Y1_05125"
     CDS             complement(931..2049)
                     /gene="recA"
                     /locus_tag="AC16Y1_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR5831875.1"
                     /translation="MAAAKGAKAVKETPSASNGTEREKALETALAQIERQFGKGSIMK
                     LGQEDRPPVESIPTGSIALDIALGIGGLPRGRVVEIYGPESSGKTTLAIHAIASVQAA
                     GGIAAFVDAEHAFDPEYAKKLGVDIDNLLVSQPDTGEQALEIADMLIRSGALDLIVID
                     SVAALVPRAEIEGEMGDSHMGLQARLMSQALRKITGALSTNNTTAIFINQLRDKIGVM
                     FGSPETTTGGKALKFYASIRLDIRRIETLKDGAEPVGNRTRVKVVKNKVAPPFKQAEF
                     DILYGEGISREGGILDMAVEEGFVRKAGAWYTYEGDQLGQGKENARAFLKDNPALTND
                     LERMIKEKLGVGVGKADLTVVPTVEAAADLDDEFADED"
     gene            complement(2259..2453)
                     /locus_tag="AC16Y1_05130"
     CDS             complement(2259..2453)
                     /locus_tag="AC16Y1_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3046 domain-containing protein"
                     /protein_id="HIR5831876.1"
                     /translation="MRLTDFWERMEQALGAGYARSWATDFHIPDLAGRTVNQALAEGE
                     DTLVVWRAVHKVLNLEAKYR"
     gene            complement(2459..3235)
                     /locus_tag="AC16Y1_05135"
     CDS             complement(2459..3235)
                     /locus_tag="AC16Y1_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831877.1"
                     /translation="MIKRLVLTFALLIPTPAFANEVVPSSEPTPQSLFDIPVSAYTPP
                     LEASQGVGGWAVVHPVTGVVHGVIVGTIETYNAWNGRMPSEYMGCPAGCLLRFQTRAT
                     ADGNVAGWHGPEVTYDSSDQTFNLGFSSQAPNGSTTVKQKLIPEKTAADGVNLSTGLV
                     DIRTEFVSTQLNGLVVKLTKIQENFESQDESIVEFQDWQSFTYQDSDEMNQNLEADVD
                     SALVAQGFDTQEPAEDGFVDTVKKLTKKITGFFGGLFSSE"
     gene            complement(3289..>3660)
                     /locus_tag="AC16Y1_05140"
     CDS             complement(3289..>3660)
                     /locus_tag="AC16Y1_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006214501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5831878.1"
                     /translation="AVQAEINRALTHTVKIDAPVKADALATVRYPKKPVILRQVIGEE
                     LRRRRMEQKRTLRQVSNLAQVSLGYLSEVERGQKEASSELLASIANALDTPLSELLAG
                     VTKEVARMEDSMSIRNDFSVI"
BASE COUNT          921 a          880 c          779 g         1080 t
ORIGIN      
        1 tgcgtcgatg ttgtgagtgc caaaaaccac gtcaacatat ggtgctttct taagaatttc
       61 accgcgatct ttttgcgcta aacagccacc gactgcaatt tttaagttct tattcttaag
      121 tttgatcggc tgcagatggc taagcgcgcc ataaagtttg ttgtctgcgt tctcacgaac
      181 tgcgcaagta ttgaaaacaa ttacatcggc aagttcgcct tctggggcag gttcataacc
      241 agcggcctct agtaacccag cgaaacgttc tgaatcatgg acgttcatct ggcagcccat
      301 agtgcgcacc tcataggtgc ctaaggcgtt agattccaga gttgaagtca caaaccaatt
      361 ctaggcaaag tcagctaggt cctgatccgc gctaaattcg cttaattgtt ctttaattac
      421 tcgaataact acacctgagt tataaccctt tcgggctagt aagccataaa gtcggcgctc
      481 tattacattt ttttctaatg aacgcattga acggagtttt ttaacaacta actcagttgc
      541 catccgatat tcatcttcat cggtcacctg cgacagtgcc tgttcaatgt gctcgtctga
      601 gatacctttt tggcgcatct ccattcgcaa aacgcttctt gatattttct tagccacaca
      661 tttacttctg acaaacattc cggcataagc caagtcatcg ataagcccaa cttcagttag
      721 tcgatccaga cttgccttag ccacttcagg atccacaccc tttttggcca gtacttgttc
      781 taattgaaac ctggtttttg gggctagtga caactggcga agggcaatgt tttttgccac
      841 agacaatgga tcagcctcgg ggttttcctt tagatctaaa ggatcactac ccgaggctgc
      901 catttgaaac atgtttacct ggactagaaa ttaatcttcg tcagcaaact catcatctag
      961 atcggctgct gcctcaacag ttggcacaac tgttaaatct gctttaccaa cgccgacacc
     1021 tagtttttct ttgatcattc gctctaagtc attggtcaat gctggattgt ctttcaagaa
     1081 agcacgagca ttttctttac cttgacctaa ttgatcgcct tcgtaggtat accaagcacc
     1141 agctttgcga acaaaacctt cttcaacggc catgtctaaa atgccacctt cgcgggaaat
     1201 accttcaccg taaagaatgt cgaattctgc ttgcttgaat ggtggtgcaa ccttgttttt
     1261 aacaactttt acccgagtgc ggttacctac tggttcagca ccatctttaa gtgtttcaat
     1321 tcgacgaatg tctaatcgaa ttgaagcgta gaacttaagc gccttaccac cggttgttgt
     1381 ttcaggtgaa ccaaacatca cgccaatttt gtcgcgaagt tggttaataa aaatcgcagt
     1441 ggtgttattt gtgcttaggg cacctgtgat tttccggagt gcttgtgaca ttaagcgagc
     1501 ttgaaggccc atgtggctat cgcccatttc accttcgatt tcagcacgtg gcacaagggc
     1561 agcaactgag tcaataacaa tcagatcaag tgcacctgag cgaattaaca tgtcggcaat
     1621 ttcaagtgct tgttcgccag tgtctggttg cgaaacaagt aagttgtcga tgtcgacacc
     1681 taacttcttt gcatattctg gatcgaatgc gtgttcagca tcaacaaacg cagcaatacc
     1741 gccagcggct tgcacacttg caattgcgtg aattgccaaa gttgttttac cggaagattc
     1801 aggaccgtaa atttctacaa cgcggccacg tggcaggcct ccaataccta atgcgatatc
     1861 gagtgcgatc gagccagttg gaattgattc aacaggtggg cgatcttctt ggccaagttt
     1921 cataattgag cccttgccaa actggcgttc aatctgggct aatgcggttt caagagcctt
     1981 ctcgcgttcg gtaccgttac ttgcacttgg cgtttctttt actgcctttg cgcctttagc
     2041 tgcagccatt gcttatctcc tactttgctt acgtatgttt ggggaattca tacgttaggt
     2101 gcgggctctg acatctagaa gactgtttgg ctggctgtgg agggttttaa cctgtgcccg
     2161 gccttacatt tcttgatgtt ttgatcgcgt aggattaatt tatcgaacaa gtgttcgaat
     2221 aaccagttag caggccttcg gcgtgttgcg gctctttttc agcggtattt cgcctctaaa
     2281 ttcagcactt tatgcacagc tcgccaaacc acaagagtat cttcaccctc ggccagagcc
     2341 tggttaacgg tccggccagc taaatctggg atgtggaaat cagttgccca tgacctggcg
     2401 tatccggccc ctagggcctg ttccatccgc tcccaaaaat cagtaagtcg catggagttt
     2461 attcgctaga gaaaaggcca ccaaagaaac cagtgatttt tttagttagc tttttcacgg
     2521 tatccacaaa tccgtcttct gctggctctt gggtgtcgaa accttgagca actaatgcgg
     2581 aatccacatc tgcttctagg ttttgattca tctcatctga atcttgataa gtaaaggatt
     2641 gccaatcttg aaattcaact attgactcat cttggctttc aaaattttct tgtatttttg
     2701 ttaacttaac aactaatcca tttaactgcg tagaaacaaa ttcagttcga atatcaacca
     2761 atccagtact tagatttaca ccatcagctg ccgttttttc tggaatcaat ttttgtttga
     2821 cagttgttga tccatttggc gcttgcgagg aaaaacctaa attaaatgtt tgatctgaac
     2881 tgtcataagt gacttcagga ccatgccaac cggctacatt tccatccgct gtggctcttg
     2941 tctggaaccg caataaacat cctgccggac aacccatgta ttcactgggc atccgtccat
     3001 tccaagcgtt gtaagtttca attgtgccga caataacccc atggactacc ccagtaaccg
     3061 gatgaactac tgcccatccg ccaactcctt gactcgcctc tagcggaggg gtgtaagcag
     3121 agacaggaat atcaaaaaga gattgtggtg ttggctcgct ggatggtact acctcatttg
     3181 caaatgctgg tgtcggaatc agcagtgcaa atgtcaaaac taatcgttta atcaatttgg
     3241 cgcctcaagt ccgtcaatta cttgatccta ctgccagttt tgttgcgatt aaatgactga
     3301 gaaatcgttg cgaatgctca tgctatcttc catgcgagca acctctttag tcacgccagc
     3361 aagtagttca gaaagcggag tatccagagc attggcaatc gaagcaagga gttctgacga
     3421 agcctctttt tgaccgcgtt caacttcact taaataacca agggaaactt gggcaagatt
     3481 tgaaacctga cgaagggtgc gcttttgttc cattcggcga cggcgaagtt cttcaccaat
     3541 cacctggcgc aaaataactg gcttttttgg atagcgaaca gtggctaggg catctgcctt
     3601 taccggcgca tcaattttta ccgtatgggt aagcgcgcga ttaatttcag cttgtactgc
//
LOCUS       DBNHVN010000271         1075 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1761243, whole genome shotgun sequence.
ACCESSION   DBNHVN010000271 DBNHVN010000000
VERSION     DBNHVN010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1761243"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..106)
                     /locus_tag="AC16Y1_05145"
     tRNA            complement(36..106)
                     /locus_tag="AC16Y1_05145"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(72..74),aa:Gln,seq:ttg)
     gene            264..926
                     /locus_tag="AC16Y1_05150"
     CDS             264..926
                     /locus_tag="AC16Y1_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019986058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5831879.1"
                     /translation="MSQNDDLDTTPRPRSTDADRVRLTGHERRAQLVEVGRALFAEKG
                     FEAVTIEEIAASANVSKPVVYEHFGSKDGLYAVVVDREMNYLLNSITKALTARHPRLL
                     LEQAACALFDYIESNTDGFQILLRDSPVATASGSFASLLSDVAARVEDLLVTEFSSRG
                     FPKNMAPMYAHMFVGMVALTGQWWLDAPRTKKDEVIAHMVNLVWNGARGLETNPRLIT
                     RR"
BASE COUNT          288 a          262 c          243 g          282 t
ORIGIN      
        1 tcgtgatcgg acgccgttgg cgtcaagcga ctcagctccc ggacgtggac tcgaaccacg
       61 ataagcacct ccaaagggtg ctgtcctgcc attagacgat ccgggattac ctgcacaaaa
      121 acaaaaatgt ttttcgtgcc ttccaaatcc taggcggact aaggccattt cccaaaatac
      181 tgacccaaaa aatgttgcag taccctgtat aaataaagca cgcgtaaaag acggcgaaat
      241 gtagttaaag gaaattcgtg gcaatctctc aaaacgatga cctagataca acacctcgcc
      301 cacgcagcac agatgctgat cgcgtgcgcc tgactggtca tgagcgtcgc gcacaacttg
      361 ttgaagtagg ccgtgcactt tttgccgaaa aaggttttga agctgtaacc attgaagaaa
      421 ttgcggcaag tgcaaacgta tctaaaccag ttgtctacga acactttgga tcaaaagatg
      481 gtttgtatgc agtcgtagtc gaccgcgaaa tgaactactt actcaactcg attactaaag
      541 cactgacggc taggcaccca agacttttac tagaacaagc tgcatgcgcg ctatttgatt
      601 acattgaatc caatactgat ggtttccaga ttttgcttcg cgatagtccg gtggcaactg
      661 cctctggctc atttgccagt ttgctttctg atgttgctgc tcgagtcgaa gacttactag
      721 ttactgaatt ttctagtcga ggttttccaa aaaatatggc tccgatgtac gcccacatgt
      781 tcgttggcat ggtggctcta actggtcagt ggtggttaga tgcaccgcgc actaaaaaag
      841 acgaagttat tgcacacatg gtgaatctag tttggaatgg cgctcgcggc ttagaaacca
      901 atccccgatt aattactcgc cgctaatttc ttcttggtca aagggccgca caatatctcg
      961 cagcacatct gctaactgat tgcgctcggt ttttgaaatc cccgctagca aaattctttc
     1021 ccgctcaagt aagtcagtta aggcagcgtc tacggcattt tgtccagtcg ttgtt
//
LOCUS       DBNHVN010000272         1238 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1092332, whole genome shotgun sequence.
ACCESSION   DBNHVN010000272 DBNHVN010000000
VERSION     DBNHVN010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1238)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1092332"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..>1238
                     /locus_tag="AC16Y1_05155"
     CDS             10..>1238
                     /locus_tag="AC16Y1_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5831880.1"
                     /translation="MRKIVIIGAGIVGCSLADELTERGELDVTVIEQGPMPAPGGSTS
                     HAPGLVQKVTANKTLAIMADQTINKLLSVETEEGPGFYQVGSLEVATTPERLKELNRR
                     VAWLKAYGFEAEVISPQRAAELHPVLDPTKILGAAYCPTDGLAKGVRAVQALQRLAES
                     RGAIFKDRHEVIAIEHENGKVTGVTTDQGFFPADIVVSCAGIWGPKVGRMFGMQKAFQ
                     PLAHQLAWTEDIPSLADQTEEITIPIMRHQGADLYYRQKFKGMGIGSYLHRALPVESE
                     EILPFDQAPVMPSQLEFTQPEWEPVMGLSAQIMPELAEAKITDGMNGMFSFTVDTMPL
                     MGEWNGLKGFWVAEAVWVTHSVGVARATAEWIIEGHPTLPVHECDVNRFEPHQLGPNH
                     IKMRGSQNYIEVYDIIHP"
BASE COUNT          348 a          280 c          293 g          317 t
ORIGIN      
        1 cgacctgcag tgcgcaaaat tgtaattatc ggagccggca tagttggttg ttcacttgct
       61 gatgaactaa ctgaacgtgg tgagttagat gtaaccgtca tcgaacaagg tccgatgcca
      121 gcacctggtg gttcaacttc acacgcacct ggtttagtac aaaaagttac tgctaataaa
      181 actttggcca tcatggctga tcaaacaatt aataaattac tttcagttga aactgaagaa
      241 ggtccaggtt tttatcaagt tggctcatta gaagttgcca caaccccaga acgactaaaa
      301 gaattaaatc gtcgagtagc atggttaaaa gcatatggat ttgaagcgga agttatttcg
      361 ccacagcggg ctgccgaact ccaccctgtg cttgatccaa caaaaatttt aggtgctgct
      421 tactgtccta ccgatggttt agctaaaggc gttcgggctg tccaagcact acagcggcta
      481 gccgaatcac gtggtgccat ttttaaagat cgccacgaag ttattgcgat cgaacatgaa
      541 aatggaaaag taaccggtgt tacaactgac caaggatttt ttccagctga catagtagtt
      601 tcttgcgctg gcatctgggg accaaaagtt ggtcggatgt tcggtatgca aaaagcattt
      661 caaccgctcg cccaccaact tgcttggact gaagacattc catcactagc ggatcaaaca
      721 gaagaaatta ctattccgat catgcgccac caaggtgctg acctttacta ccggcaaaaa
      781 ttcaaaggta tgggtattgg ttcctacttg caccgagcac tgccagttga gtctgaagaa
      841 attttgccat tcgatcaagc gccggttatg ccaagtcagc ttgaattcac ccagcctgag
      901 tgggagccgg tgatgggtct ttcggcacag attatgcctg aattagctga agcaaaaatt
      961 actgacggca tgaacggtat gttctcattc accgtagaca ctatgccgtt aatgggtgaa
     1021 tggaatggac tcaaaggttt ctgggtggct gaggctgttt gggtaaccca ttcagtgggc
     1081 gttgcccggg ccaccgctga atggattatt gaaggacatc caaccttgcc ggttcatgaa
     1141 tgcgacgtaa atcgcttcga accacatcaa ctgggaccaa accacatcaa gatgcggggt
     1201 tcacaaaact acatcgaggt ttacgacatt attcatcc
//
LOCUS       DBNHVN010000273         1642 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_53505, whole genome shotgun sequence.
ACCESSION   DBNHVN010000273 DBNHVN010000000
VERSION     DBNHVN010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_53505"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..478
                     /locus_tag="AC16Y1_05160"
     CDS             101..478
                     /locus_tag="AC16Y1_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR5831881.1"
                     /translation="MQRNTKQRQSVENCLSNYDSFVSIQEIHKELAARGEKIGLSTVY
                     RIIGDMHDENKVDMILSESNESLYRLCSKVHHHHLVCIDCGTAVELVDDLIESWANQI
                     AKSNKFKLLDHQVELTGTCAKCA"
     gene            complement(475..1242)
                     /locus_tag="AC16Y1_05165"
     CDS             complement(475..1242)
                     /locus_tag="AC16Y1_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIR5831882.1"
                     /translation="MQPLKHSSGAKPPKLPKELIPKHVAIVMDGNGRWAKERGLPRTA
                     GHEAGEHSLLDCVHGALELGITHLSAYAFSTENWRRSPDEVRFLMNFNRDVIHRRVDE
                     MHELGVRVRWAGRRPKLWKSVISELENAETLTKRNKNLTLTMCVNYGGRAEIADAMTN
                     IARDVKRKKLDPEKITERTIAKYLDEPDLPDVDLFVRSSGEQRISNFLLWQSAYAEFV
                     FMDTLWPDFDRRHLWQACEIFASRDRRYGGAVPNTPN"
     gene            complement(1255..>1642)
                     /gene="recO"
                     /locus_tag="AC16Y1_05170"
     CDS             complement(1255..>1642)
                     /gene="recO"
                     /locus_tag="AC16Y1_05170"
                     /inference="COORDINATES: protein motif:HMM:NF014607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR5831883.1"
                     /translation="GALRTLVSGEHDATLVLNAYLLRALSLAGYSPNLDACIVCGNFG
                     TQPFFHVPSGGALCFEHRAPGSVAPKAQSIEIMQALLQGDWETADRATTAHLNEISGI
                     VAAFTQWHLEKSLRSLPLVDRTSNLS"
BASE COUNT          461 a          379 c          348 g          454 t
ORIGIN      
        1 tgcccgaagc gcattcaaat aaaccagcaa agtcaacatt tgtttcaact ttcgcaggtg
       61 tgtttgtaat gtttgcacta gtttcattac tccactaaaa atgcaacgaa atacaaaaca
      121 acgtcagtct gttgaaaact gtttgagcaa ctacgacagc tttgtcagca ttcaagaaat
      181 ccacaaagaa cttgctgcca ggggcgaaaa aattggctta tcgaccgtct accgaataat
      241 tggtgacatg cacgatgaga acaaagtcga catgatccta agtgaatcaa atgagtcact
      301 atatcgcctc tgtagcaaag ttcatcatca tcatttagtt tgtattgatt gtggcactgc
      361 ggttgaacta gttgatgact taattgaatc ttgggctaat caaattgcta aatcgaataa
      421 gttcaaactc cttgaccatc aagttgaact cactggcaca tgtgcaaagt gtgcttaatt
      481 aggcgtattg ggtactgctc cgccataacg gcgatcgcga ctggcaaata tttcacaagc
      541 ttgccatagg tgccggcgat cgaaatctgg ccagagcgtg tccatgaata caaattcagc
      601 ataggcactt tgccagagta agaaattact tattcgctgt tcaccgcttg atcggacaaa
      661 taggtcgaca tcaggaagat ctggctcatc taaatactta gcaatcgttc gctcagttat
      721 tttctctgga tctagctttt tgcgcttaac atcgcgggca atattggtca tcgcgtcagc
      781 tatttcagct cgaccgccat agttaacaca catagttagg gtgaggttct tattgcgctt
      841 ggttagcgtt tcggcatttt ctagttcaga aataactgat ttccaaagtt tcgggcggcg
      901 accggcccat cgaactcgaa caccaagttc atgcatctca tcaactcgcc gatgtattac
      961 atcgcgattg aaattcatta agaagcgaac ttcgtctggc gatctacgcc aattctctgt
     1021 tgaaaacgca taagccgata aatgtgttat ccctaattcc aaagcgccat gcacacagtc
     1081 gagcaaactg tgttcgccag cttcatgacc agcagttctt ggcaagccac gctctttagc
     1141 ccatcggccg tttccatcca tcacaattgc cacgtgtttg ggaattagct cttttggcaa
     1201 ctttggtggc tttgcaccag aactatgttt aaggggttgc atccagatat cttttcatga
     1261 aaggttgcta gttcgatcaa ctagcggcaa agaacgaagc gatttttcta aatgccactg
     1321 cgtgaaggca gccacgatcc cagaaatttc atttaggtgt gcagttgtgg cccgatcagc
     1381 tgtctcccaa tcaccttgta gtaacgcttg cataatttca atactttgcg ctttgggtgc
     1441 aacacttcct ggagctcgat gctcaaaaca aagtgcgcca cccgagggca catggaaaaa
     1501 tggttgggta ccaaaatttc cacaaactat gcaggcatca aggtttggcg aataaccggc
     1561 aagcgataat gcgcgtagta aatatgcatt taaaactaat gtggcatcat gttcgccact
     1621 tactaaagtg cgcaatgctc ca
//
LOCUS       DBNHVN010000274         2660 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_199540, whole genome shotgun sequence.
ACCESSION   DBNHVN010000274 DBNHVN010000000
VERSION     DBNHVN010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_199540"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /gene="gcvPB"
                     /locus_tag="AC16Y1_05175"
     CDS             <1..1475
                     /gene="gcvPB"
                     /locus_tag="AC16Y1_05175"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018558701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HIR5831884.1"
                     /translation="QDVFHKYRSETEMMRYLRRLSEMDIALDRAMIPLGSCTMKLNAT
                     SEMEAITWPEFANIHPFAPLSTVSGYLTLIKQLEEWLVEVTGYDAVSLQPNAGSQGEF
                     SGLLAIRSYHMARGEGHRDICLIPSSAHGTNAASAVMAGMKVVVVGCDDFGNVDMDDL
                     KHKIMENESRIAALMVTYPSTHGVYESSITDICAMVHDAGGQVYVDGANLNALVGLAK
                     PGKFGADVSHLNLHKTFAIPHGGGGPGVGPVAVREHLAPYLPSHPIRPEAGPLGRGHA
                     DGGVGPVSAAPWGSAGILTIPWAYIRMMGGAGLTKATQVAVASANYIAKNLADDYPIL
                     YVGANNLVAHECILDLREITKETGVTVDDVAKRLIDFGFHAPTMSFPVAGTFMVEPTE
                     SESLFELDRFIAAMKQIKQEILQVGKGKWTVEDNPLANAPHTAEYCLGEWDHPYSREE
                     AAYPVASLRNQKYWPPVRRIDGAYGDRNLVCACPPITDFM"
     gene            1522..>2660
                     /locus_tag="AC16Y1_05180"
     CDS             1522..>2660
                     /locus_tag="AC16Y1_05180"
                     /inference="COORDINATES: protein motif:HMM:NF016310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HIR5831885.1"
                     /translation="MLSLSEQEEAMLAGDLGQGIALAMKLVVELGRIKGAKKLIDIES
                     VHVDGCLFYGQAGLDYAQKLVDLGARVVVPTTVNVGKVDLLHPNLVRQDTDKEKFMAS
                     GGRKLMDAYAQLGARQTWTCAPYQQDTRPEFGSDIVWAESNAIAFANSVLGARTDRYG
                     DFMDIASAITGKTALAGLHLPENRVGHLILDCRALSKRVLELDITYPILGYLAGKFAG
                     TRNPVFLGIPDDVSEDKLKALGAAAASAGGVGLFHVVGRTPEAMTLEQVVANQNDIPT
                     FFITGDLLAKTRDELSTAKQNAKLDAVSLGTPHASLDELEQLCKAVSGGPNINSEVDF
                     YINTGRSVFNSAKDLGLIDQLEKRNVKFVVDTCAYVTSIFRPGTKT"
BASE COUNT          735 a          551 c          663 g          711 t
ORIGIN      
        1 ctcaagatgt gtttcacaaa taccgaagtg aaaccgagat gatgcgctac ttgcgacggc
       61 tgtcagaaat ggatatcgcc ctcgatcgcg cgatgattcc gcttggttca tgcacgatga
      121 aactaaatgc gacatcagag atggaagcca ttacctggcc agaatttgcc aacatacatc
      181 cgttcgcacc acttagcact gtgtccgggt atttaacgtt gattaaacaa ctagaagaat
      241 ggcttgtcga agtaactggc tatgacgccg ttagtttgca accaaatgct ggctcacaag
      301 gagagttttc tggactcctt gccattcgca gttaccacat ggctcgcggc gaaggacatc
      361 gagatatttg tttgatccca agttcagcac atggcacaaa tgctgcatcc gcagttatgg
      421 ctggcatgaa agtcgttgta gttggttgtg acgattttgg caatgttgac atggatgacc
      481 taaagcacaa gattatggag aacgaaagtc ggattgctgc cctcatggtc acttatccgt
      541 ccacacatgg tgtctatgaa tcttcgatta ccgacatttg tgcaatggtg catgatgcag
      601 gtggtcaagt ttatgttgac ggtgcgaatt taaatgcact tgtaggttta gccaaacccg
      661 gcaagtttgg cgcagatgtt tctcatttga acttacataa gacatttgcg atcccacacg
      721 gtggtggtgg acctggcgtt ggtccagttg cagtgcgcga gcacttagcg ccgtaccttc
      781 catcccaccc gattagacca gaagctggtc cgttgggtcg cggtcatgcc gatggtggtg
      841 taggaccagt tagtgcggca ccctggggta gcgcaggaat tctcacaatt ccatgggcat
      901 acattcgcat gatgggggga gcagggctaa caaaagcaac gcaagttgca gtggccagtg
      961 ctaactacat tgccaaaaat ctagctgatg attacccaat tctttatgtt ggtgccaata
     1021 acttagttgc gcatgaatgt attttggatt tacgcgaaat tactaaggaa actggtgtga
     1081 cagtagacga tgtggcaaag agattaatag atttcggctt ccacgcgccg acaatgtctt
     1141 tcccagttgc cggaactttc atggttgaac ctactgaaag tgaatcctta tttgagttag
     1201 atcgatttat tgctgcaatg aagcaaatca aacaagaaat acttcaggtt ggaaagggta
     1261 agtggacagt tgaagacaat ccgcttgcga atgcaccaca tacagctgag tattgcctag
     1321 gtgaatggga tcacccgtat agccgagaag aagctgctta cccagtggca agtttgcgca
     1381 atcaaaagta ttggccacca gtaagacgaa ttgatggtgc ttatggtgat cgcaatttag
     1441 tttgtgcatg cccgccaatt acggatttca tgtaatactc aatctcgcaa aagaaagcac
     1501 ctacttgggt aacctgagta ggtgctttct ttatctgagc aagaagaggc aatgctggca
     1561 ggggacctgg gccaaggcat tgcattggcc atgaagttag tcgttgaatt aggacgtatt
     1621 aagggcgcca agaagctaat cgatattgaa tcagtccatg ttgatggttg cttgttttat
     1681 ggtcaagccg gtttagatta cgcgcagaag ttagttgatc taggcgccag ggtagtggtt
     1741 ccaaccacag ttaatgttgg aaaggttgat ctacttcacc cgaatctggt ccgacaagat
     1801 actgataaag agaaattcat ggcatcgggt ggtcgtaaat taatggatgc ttacgcgcag
     1861 cttggcgcta gacaaacttg gacctgtgcg ccttaccaac aagacacacg tcctgaattt
     1921 ggctctgaca tagtttgggc tgaatcaaac gcaatagctt ttgctaattc tgttttaggt
     1981 gcaagaacag accgttacgg cgactttatg gacattgcta gtgcaataac tggtaagact
     2041 gcactagcag gtctacatct accggaaaat cgagtcggtc atttaatttt agattgccgc
     2101 gcactttcta aacgtgttct tgaactagac attacatatc cgattctggg atatttagct
     2161 ggaaaattcg ctggtactcg aaatcctgtt tttctcggta tcccagatga tgtaagtgaa
     2221 gacaagttga aagctttagg tgctgctgca gcatctgcag ggggagttgg actctttcac
     2281 gttgttggaa gaacgcctga ggcgatgaca cttgaacagg tagtggctaa tcaaaatgac
     2341 ataccaactt tctttataac tggtgattta cttgccaaaa ctagagatga actaagtacc
     2401 gccaaacaaa atgcgaaatt ggatgcggtg tcgctgggaa caccacatgc ttcgctagat
     2461 gagttagaac agctgtgcaa agcagttagt ggtggaccca acatcaattc agaggttgat
     2521 ttctacatca atactggacg tagtgtattc aattccgcaa aggatctagg tctgatagat
     2581 caacttgaaa agcgaaatgt gaaatttgta gtcgatacct gcgcatacgt aacatcaatt
     2641 ttccgaccag gaacgaaaac
//
LOCUS       DBNHVN010000275         1115 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_719866, whole genome shotgun sequence.
ACCESSION   DBNHVN010000275 DBNHVN010000000
VERSION     DBNHVN010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_719866"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /gene="recF"
                     /locus_tag="AC16Y1_05185"
     CDS             <1..1068
                     /gene="recF"
                     /locus_tag="AC16Y1_05185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.2"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR5831886.1"
                     /translation="LEKFRNHQNLKLDFIGPTTTIVGRNGRGKTNIVEAVNYFATLGS
                     HRVATDQPLIQNQETTSEITGVFEKHGRVATAKITINSSGQNKVFLNSSPLTKPRDLL
                     GLVSAVIFSPEDLDLVKGDPSVRRKYLDDFSVLLSPKFASVRSDYDRVLKQRNTLLKS
                     AGRRALADSAQETLKVWNEQLVKFGSDIVTQRLAALAKLTPFIQQHGATLSADNEPLE
                     INYFAKWFNDEKNIEQQFKDSLAEKQNEELDRGVTLVGPHRDELELKLSGMPAKGYAS
                     HGQSWSIALAMRLATFNVLREHDDDPILILDDVFAELDQRRRTRLLASIGGVEQTLIT
                     AAVAEDLPEGLAGEQILLEDY"
BASE COUNT          361 a          240 c          228 g          286 t
ORIGIN      
        1 ttagaaaaat ttagaaatca tcaaaaccta aaactagatt tcattggccc aaccacaaca
       61 attgttggcc gaaacggtcg aggcaaaacc aacatcgtcg aggcagttaa ctactttgca
      121 accctaggta gtcatcgagt ggccacagac caaccgctga tccaaaatca agaaactacc
      181 tcggaaataa caggtgtttt tgaaaaacac ggccgggtgg cgacggcaaa aatcaccatc
      241 aatagcagtg gtcaaaacaa ggttttttta aacagcagcc cactaactaa acctcgcgac
      301 ttactcggtt tggttagtgc tgttattttt tcacccgaag atttagatct agtaaaaggt
      361 gacccaagtg tgcgccggaa gtacctagat gatttttcag ttttattaag tccaaaattt
      421 gccagcgtta gatcagatta cgatcgagtt ttaaaacaaa gaaacacgct tctgaaatca
      481 gccggccgtc gcgcacttgc cgattcagcc caagaaactt taaaggtttg gaatgagcaa
      541 cttgttaagt ttggtagcga catagtcact caacggttag ctgctttagc aaaactaact
      601 ccattcattc aacaacacgg agcaacatta agtgccgaca acgaaccact agaaattaat
      661 tactttgcaa aatggtttaa tgatgaaaaa aatatcgagc aacaatttaa agactcgtta
      721 gccgaaaaac aaaatgagga gttagatcgc ggagttactc tagttggtcc acatcgggat
      781 gaactggaac taaaactaag cggtatgcca gctaaaggtt atgcatcaca tggtcaatct
      841 tggagtatcg ctcttgccat gcgacttgcc acttttaatg ttttgcgcga acacgacgac
      901 gatccaattt taattttgga tgatgtcttt gctgaattag atcagcgaag aagaactcga
      961 ttacttgcct cgattggcgg tgtcgaacaa accctaatta ctgccgccgt agccgaagat
     1021 ttacccgaag gactagccgg cgaacaaatt ttgttagagg attactagat cgtgttaact
     1081 gcagaacaac aagaattctt gcgctcctta ttatc
//
LOCUS       DBNHVN010000276         3028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_2058481, whole genome shotgun sequence.
ACCESSION   DBNHVN010000276 DBNHVN010000000
VERSION     DBNHVN010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_2058481"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="AC16Y1_05190"
     CDS             complement(<1..418)
                     /locus_tag="AC16Y1_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831887.1"
                     /translation="MVEITKAINKKFFPLISFVPYLVLTPVLYILVSLYAMVFPPLDV
                     AVYKRAADFVFAGMSPYSPEFITGDVYLPWVYTPFGTLVLLPLHFVSESNLLIFWSIF
                     GILIPLLVLVLVGYQGLLSRINLSRKEKLALIILLTF"
     gene            complement(536..979)
                     /gene="rplI"
                     /locus_tag="AC16Y1_05195"
     CDS             complement(536..979)
                     /gene="rplI"
                     /locus_tag="AC16Y1_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006348077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR5831888.1"
                     /translation="MKLILTQEVNGLGAAGDVVEVKAGYGRNFLMPRGLATAWTRGGE
                     KQVTQIKRARTKRQVRDREHALEVKAALESAPVVLTARAGDEGRLFGSVTVSDIAAAI
                     KNVEVDKRKIVLSAPIKLAGKHNVSVRLHPEVVADVVVDVRGKKS"
     gene            complement(994..1230)
                     /gene="rpsR"
                     /locus_tag="AC16Y1_05200"
     CDS             complement(994..1230)
                     /gene="rpsR"
                     /locus_tag="AC16Y1_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003949403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR5831889.1"
                     /translation="MAKPVIRKIKPKACAFCREDNAVIDYKDIALLRKFISDRGKIRA
                     RRVTGNCTQHQRDIANAVKNAREIALLPYTSTAR"
     gene            complement(1292..1750)
                     /locus_tag="AC16Y1_05205"
     CDS             complement(1292..1750)
                     /locus_tag="AC16Y1_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR5831890.1"
                     /translation="MALGDVTVTVTGNLTSDPELRFTPNGSAVASFTVASTTRVLDRD
                     SNQWKDGDTLFLRCSVWRQYAENVAESLTKGTRVIVTGRLKQRSYETKEGEKRTVVEM
                     DVDDVGPALKNATAKVNRITRSSDGGYNGGSSAPADDPWAAPVSNDEPPF"
     gene            complement(1762..2052)
                     /gene="rpsF"
                     /locus_tag="AC16Y1_05210"
     CDS             complement(1762..2052)
                     /gene="rpsF"
                     /locus_tag="AC16Y1_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011738469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR5831891.1"
                     /translation="MRDYEVVVILDPELEERTVAPSLDTFLNVVKKDGGTVRKVDIWG
                     RRRLAYEIDKKGEGIYALLDVNCSPAAVAELDRQLGLNESVLRTKVMRVDSK"
     gene            2187..2969
                     /locus_tag="AC16Y1_05215"
     CDS             2187..2969
                     /locus_tag="AC16Y1_05215"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HIR5831892.1"
                     /translation="MTKLAIGSHVSNENPIETAKNLGLETIQIFVADPQGWKGTEIPH
                     PDGGEGLKKAAKAAKVDVYVHARYIINVATTNNKVRIPSRKILQADMDAAAELGAKGL
                     IVHGGHVTADDDPAVGVANWVKAIDQLDMKIPLLLENTAGGKFSMARRLESIARLWDE
                     IGDKGVGFCLDTCHAHAGGIEQVGLAETILKTTGRIDLVHCNNSRDAFDSGADRHANL
                     NSGTMDVAVLIQNLKKANAPIILETPYEDIETDLKILRDALG"
BASE COUNT          867 a          659 c          656 g          846 t
ORIGIN      
        1 agaaagttaa cagaattatc aaagcaagtt tttcttttcg cgataaatta attctgctaa
       61 gtaatccttg atacccaact agaaccaaaa ccagcagcgg tatcaagatg ccaaaaatac
      121 tccagaagat taataaatta gattctgaga caaaatgcag aggtaataac actaatgtgc
      181 caaaaggcgt gtacacccaa ggtaaataaa catcaccagt tataaattct gggctatacg
      241 gtgacatacc ggcaaaaaca aagtcagcag cgcgtttgta aactgctaca tccagtggtg
      301 ggaaaaccat tgcataaagc gaaacaagga tgtacagaac tggagttaat accagataag
      361 gaacgaaact tattaatgga aaaaatttct tatttattgc ttttgtaatt tcaaccatag
      421 ttctctaatt tttatatctt ggatctcaga ttagtagtta agcgtttcca aaaaaaggaa
      481 accataaaaa tgctggggcc attcagttga atgaccccag cgaatattta attaattagc
      541 tcttcttacc gcgaacatca accacaacat cagcaactac ttctgggtga aggcgtactg
      601 aaacattgtg tttgcccgct aatttaatcg gtgcagaaag tacaattttg cgcttatcta
      661 cttcgacatt cttgattgca gcggctatgt cacttacagt tactgaaccg aacaggcgac
      721 cttcatcacc agcacgtgcg gtaagtacaa ctggtgctga ttctaaagcg gccttaactt
      781 ctagtgcatg ctcacgatcg cgaacctgac gtttagtgcg agcgcgctta atttgcgtaa
      841 cttgcttttc gccaccgcgt gtccacgctg tagctagtcc gcgtggcatt aagaagttgc
      901 gaccgtaacc ggccttaact tcaactacat cgcctgcggc acctagacca tttacttctt
      961 gagtcaaaat aagtttcatt ttctaaatgc tccttatctt gctgtcgatg tgtaaggcaa
     1021 aagtgcaatt tcgcgcgcat tctttactgc gtttgcgata tcgcgctggt gctgggtgca
     1081 gttaccggtt actcggcgag cgcggatctt gccgcgatct gaaataaatt tgcgaagtag
     1141 tgcaatatct ttgtaatcga ttactgcatt atcttcacga caaaaagcac aagcttttgg
     1201 tttgatcttg cgtattactg gctttgccat tgtggtgctc ccttttgagc ccagatttct
     1261 ctggaatggt tactaatttt taaagtgata cctagaaagg tggttcgtca ttcgaaactg
     1321 gagcagccca tggatcatca gccggtgcac ttgagccacc gttgtatcca ccatcgctgc
     1381 tacgagtaat tcgattaact tttgcggttg catttttcaa tgctggaccg acatcgtcaa
     1441 catccatttc aactacagtg cgcttttcgc cttctttagt ctcgtacgaa cgttgcttta
     1501 gtcgcccagt cacaatgacg cgggtgcctt tagttaaaga ctccgctaca ttttctgcat
     1561 actgacgcca aacgctacaa cgtaagaaaa gggtgtcgcc atctttccat tgattggaat
     1621 cgcgatctaa aacacgagtt gtggaagcca cagtgaatga agctactgct gaaccattag
     1681 gggtaaagcg cagttcggga tcactagtta aatttccagt aacagttact gttacgtcac
     1741 caagtgccat gaggtctcct cttacttact gtctacccgc atgactttag tgcgtagcac
     1801 tgactcattc aggcctagtt gacggtcgag ttctgcaact gctgctggtg aacagttgac
     1861 gtcaagcagg gcatagatac cttcaccctt tttgtcgatt tcatatgcca aacggcgacg
     1921 accccaaata tcgactttgc gaactgtgcc accatctttt ttgaccacat ttagaaatgt
     1981 gtccaaagat ggagcaacgg tgcgctcttc gagttcggga tcaaggatta caactacttc
     2041 gtagtcacgc atacttaata cccacctcct atggacttaa tcggccatgg tctttccatg
     2101 gcaggaggga ttaagaatcc caattctatg aggttttttc atcggttaaa aacgggttat
     2161 cagccctgat taggtagcct taggacatga caaaactggc tattgggtct catgtaagta
     2221 atgaaaatcc cattgaaaca gccaaaaatc taggcctcga gacaattcag attttcgtcg
     2281 ctgacccaca aggttggaag gggaccgaaa tcccccatcc agatggtggc gaaggtttaa
     2341 agaaggcagc caaagcagcc aaagttgatg tctacgtaca cgccagatac atcattaatg
     2401 tggcgacaac caataacaag gtgcggatac caagtcgcaa aatacttcag gcggacatgg
     2461 atgcagccgc tgaacttggc gccaaagggt taattgttca tggcggccat gtcaccgcag
     2521 atgatgatcc ggcagttggc gttgcgaact gggttaaagc tattgatcaa cttgatatga
     2581 aaataccgtt gctacttgaa aatactgctg gtggaaaatt ttcgatggct cgtcggttgg
     2641 aatctatcgc tcggctttgg gatgaaattg gtgacaaggg agttggtttt tgcctcgaca
     2701 cttgtcacgc tcatgccggt ggaattgaac aagttggatt agccgaaaca attttaaaaa
     2761 caacaggtcg aattgattta gttcattgca ataacagtcg cgatgctttt gattccggtg
     2821 ctgatcgaca tgcaaattta aattcaggca cgatggatgt tgctgtttta attcaaaatc
     2881 tgaaaaaagc taacgcgcca attattttag aaactccata tgaagatatc gaaactgatt
     2941 taaaaatttt gcgagatgca cttggctaaa aatgtatctg gggtaaccaa atgattctag
     3001 ttaagcaaat tagtgcagca gagcattt
//
LOCUS       DBNHVN010000277         2030 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1612254, whole genome shotgun sequence.
ACCESSION   DBNHVN010000277 DBNHVN010000000
VERSION     DBNHVN010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1612254"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /gene="sucD"
                     /locus_tag="AC16Y1_05220"
     CDS             complement(<1..694)
                     /gene="sucD"
                     /locus_tag="AC16Y1_05220"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020135709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR5831893.1"
                     /translation="MAIFLDKNSKVLVQGMTGAEGTKHTRRMIASGTNVVAGVTPGKG
                     GQEVDGIPVFNSVGEAMEKTGANVSVVFVPPKFAKAAVVEAVDAAIPLCVVITEGIPV
                     HDTASFFQYSQNKGKTRIIGPNCPGLISPEQSNAGIIPGDITKKGRIGLVSKSGTLTY
                     QMMFELRDLGFSTCVGIGGDPIIGTTHIDCLAAFQNDPDTDVIVMIGEIGGDAEERAA
                     AFIKENVTKPVVG"
     gene            complement(704..1879)
                     /gene="sucC"
                     /locus_tag="AC16Y1_05225"
     CDS             complement(704..1879)
                     /gene="sucC"
                     /locus_tag="AC16Y1_05225"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIR5831894.1"
                     /translation="MDLYEYQAKELFGAHGVPVVPGFVCTTPDEAKVAATKIGDMVVV
                     KAQVKTGGRGKAGGVKLAQNAGEAQERAKGILGLDIKGHIVHKVLVTAAAEIAEEYYV
                     SFLLDRSNRTFLAMASVEGGMDIEEVAATKPEALAKIRVDAIEGCTAEKAAEIVDAAK
                     FPAEVRDQVISILQKLWEVLIKEDATLVEVNPLAKTPDGKVLALDGKVTLDDNAQFRH
                     ESHAALIDNSTTDSIELRAKEFDLNYVKLQGEVGIIGNGAGLVMSTLDVVAYAGEEFG
                     GVKPANFLDIGGGASAQVMANGLDIVLGDPEVKAVFVNVFGGITACDAVANGIVQAIG
                     MLAERGEPVTKPIVCRIDGNNAAEGRRILDESGIAVIERVETMDDAARRVAQLAAGK"
BASE COUNT          540 a          511 c          383 g          596 t
ORIGIN      
        1 agccaacaac tggctttgtg acattttcct ttatgaaagc agcagcccgc tcttcagcgt
       61 caccgccaat ttcaccaatc attacgatga catctgtatc cggatcattt tgaaatgctg
      121 ctaagcaatc aatatgtgtt gttccaataa ttggatcgcc accgatgcct acgcaagttg
      181 aaaaacctaa atctctcaac tcaaacatca tttgataagt gagagttcca gattttgaaa
      241 ctaaaccaat tcgacctttt ttcgttatgt caccaggaat aattccagca ttagattgct
      301 ctgggctgat caagccaggg cagtttggtc caataattct ggtcttacct ttgttctgcg
      361 aatactgaaa aaatgacgca gtatcgtgaa caggaattcc ttcagtgatt acgacgcaaa
      421 gtggaattgc cgcatcaacc gcttcgacta cagctgcctt tgcaaacttt ggtggcacaa
      481 acaccacaga cacatttgcc ccagtttttt ccatggcttc acctacggaa ttaaaaactg
      541 gaattccatc aacttcttgc ccgccttttc caggggttac accagcgaca acgttggtac
      601 ctgaagcgat catgcgacga gtgtgctttg tgccttctgc accagtcata ccttgtacta
      661 atactttgct gtttttatct aagaaaattg ccattacaaa tccctactta ccggcagcca
      721 gttgtgcgac tcggcgtgct gcatcatcca tagtctcaac gcgttcaata actgcaattc
      781 ccgattcatc aagaatgcga cgaccttcgg ctgcattgtt tccatcaatc cgacaaacga
      841 ttggttttgt gaccggttct ccacgttcgg caagcattcc gatagcttgc acgattccgt
      901 ttgccacagc atcgcaagca gtaattccac caaagacgtt cacaaaaact gcttttacct
      961 ccgggtcacc aagcacaata tctaagccgt tagccataac ttgtgcgcta gcgccaccac
     1021 cgatgtctaa aaagtttgca ggctttacac cgccaaactc ttcaccagcg taagcaacca
     1081 catccagtgt ggacattact aagccagcgc cattaccaat aattccaact tcaccttgta
     1141 atttgacgta gttaagatca aattcttttg cccgaagttc aattgaatca gtggtcgaat
     1201 tgtcgatcag tgcagcatgt gattcatgac gaaattgcgc gttatcgtca agagttactt
     1261 ttccatcaag tgctagaact ttgccatctg gtgttttcgc tagtggattt acttcaacga
     1321 gtgttgcatc ttctttaatt aaaacttccc agagtttttg caaaattgaa ataacttgat
     1381 ctctgacttc tgctggaaac tttgctgcgt ctacgatctc tgccgctttt tctgctgtgc
     1441 aaccttcgat cgcatcaaca cgaatcttgg ccaatgcttc tggttttgtg gcagcaactt
     1501 cctcgatatc catgccacct tcaacacttg ccattgcaag gaaggttcgg tttgatcggt
     1561 caagtaaaaa agaaacgtaa tactcttcgg cgatttctgc tgcagcagta actaaaactt
     1621 tatgaactat gtgacctttg atatcaaggc caagtatacc ttttgctcgt tcctgcgctt
     1681 cacctgcatt ttgcgctaac ttaactccgc ctgcttttcc tcgaccaccc gttttaactt
     1741 gagccttaac tacaaccatg tcgccaattt ttgttgcagc aacttttgct tcatcaggtg
     1801 tagtgcaaac aaaaccaggt actacgggaa caccgtgtgc accaaataat tcttttgctt
     1861 gatattcata taggtccacg tttggaacac ttcctaatta cgggagatcg agatttcttc
     1921 ctagtccaga ttaggtcagg ctaggtaagt aagttaacgc aggttgtcga ttacccaacc
     1981 agtaagagac gaaagcggtg tacctggcgt gaaaataatt tttacgccaa
//
LOCUS       DBNHVN010000278         1330 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_795743, whole genome shotgun sequence.
ACCESSION   DBNHVN010000278 DBNHVN010000000
VERSION     DBNHVN010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_795743"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..849)
                     /locus_tag="AC16Y1_05230"
     CDS             complement(7..849)
                     /locus_tag="AC16Y1_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HIR5831895.1"
                     /translation="MAIKPNFPAGSTIVVAAFGGWSDAGACATDAINHLIDVWDGKLT
                     YELPTDDYYDFSFNRPEIETDENGKRQLTWPGTKIYEVKPTGLLDTKIYLIQGDEPNL
                     KWTVFANTLLDQIPSSESTVLITLGAMLAEVPHTRPVPISGTTSNEELALLTGYSPSN
                     YEGPTGIISVLAFEAAARNIPAASIWAALPHYVGAPPCPKGTLALVRGLEDVLDTSIP
                     VLDLVEEARAWQTGVEELATEDEEVAEYIRSLEETQDTAELPEATGEAIAREFERYLR
                     RRER"
     gene            949..>1330
                     /locus_tag="AC16Y1_05235"
     CDS             949..>1330
                     /locus_tag="AC16Y1_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HIR5831896.1"
                     /translation="MSESAEFKPGDRVQLSDTKGRKYTLVLEPGKSFFTTHGSVTHES
                     IIGKPQGVTVTSSGNMTYLALRPLLSDFVLAMPRGAAVIYPKDAAAIVGFTDIYPGAV
                     VVEAGVGSGALTCSLIRAIGETGLL"
BASE COUNT          344 a          305 c          303 g          378 t
ORIGIN      
        1 acagaattaa cgttcccgtc gccttaagta gcgttcgaat tcacgagcga tagcttcacc
       61 agttgcttca ggcagctctg cagtatcctg agtttcttct agagatcgga tgtactcagc
      121 aacctcttcg tcttcagttg ctaactcttc aacaccggtt tgccaggctc ttgcttcttc
      181 aaccagatca agaacaggaa tagaagtatc aagtacatct tcaagtccgc gcacaagtgc
      241 cagtgttcct ttggggcatg gcggagcacc aacgtaatgc ggaagtgctg cccagataga
      301 tgcggccgga atgtttcgcg ctgctgcttc aaaagctaaa accgaaataa taccggtcgg
      361 cccttcgtag ttcgagggac tataacccgt taacaacgct aattcttcgt tggaggttgt
      421 gccactaatc ggaaccggac gtgtatgtgg aacttccgcc agcatcgcac caagtgtgat
      481 tagcactgta ctttcgctgc tcggaatctg atcgagcaaa gtatttgcaa aaaccgtcca
      541 tttcaaattc ggttcatcgc cttgaattaa ataaattttt gtgtccaaaa gcccagtcgg
      601 tttaacttcg taaatttttg tgcctggcca agttagctga cgcttcccgt tttcatcagt
      661 ttcaatctct gggcgattaa aactaaaatc gtaataatca tcagttggaa gttcgtaagt
      721 taattttcca tcccagacat caattaaatg gtttattgca tctgttgcgc aggcacctgc
      781 atcgctccac ccgccaaatg cagccaccac gattgtggaa cctgcgggaa agttaggttt
      841 gattgccaaa tgatttcctt ttttattgcg acacaacctt atcccgaatg gtggcaggca
      901 gcagtttcct gggtacattg aaccctaatt tgctgaaaga atagtgcggt gagcgaatca
      961 gctgagttta aacccggaga tcgggttcag ttatctgata caaaaggccg aaaatataca
     1021 ttggttctag aaccaggtaa atcttttttt acaacacatg gcagcgtgac gcatgaatcg
     1081 ataattggca aacctcaagg tgtaactgta acttcatcgg ggaatatgac gtatttagcg
     1141 ctacgcccgt tacttagtga tttcgtattg gctatgcctc gaggtgcagc agtaatctat
     1201 ccaaaagacg ctgcggcaat agttggtttt actgatattt atccaggtgc agttgtagtg
     1261 gaggccggag ttggttctgg cgctcttact tgttcactga tcagagccat tggtgaaact
     1321 ggattactgc
//
LOCUS       DBNHVN010000279         2809 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1538479, whole genome shotgun sequence.
ACCESSION   DBNHVN010000279 DBNHVN010000000
VERSION     DBNHVN010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1538479"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1284)
                     /locus_tag="AC16Y1_05240"
     CDS             complement(1..1284)
                     /locus_tag="AC16Y1_05240"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0006569 - L-tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HIR5831897.1"
                     /translation="MITREFALDLDAKDALSKYRNQFVVQDETVCYLDGNSLGRLPKK
                     TVELINDFLMNGWGAKIVDGWADWIDKAQTTGDLIGRAALGAASGQTLAMDTTSVNFY
                     RAIRAAINARPNRKTIITDEANFPTDRYIMQGIADELGMNLVIIPNDLKAHIVGDVFS
                     NELVTAEILAPYLNQDVAVVSLSVVAYRSGALHNLSELTDLVRSHGALMVWDASHAVG
                     AVDMQFDRDGVEIAVGCTYKYGNSGPGAPAWLYLSKSIQQDLKMPIQGWFAQRDQFLM
                     GSKFEQIAGIRGFQIASPSIIGLLCIDQGFSMIEEASISAINHKASTGTQLMIDLHEQ
                     WLVELGYELVTPRDSKQRGGHISLYHPDGAKIARGLRDEMKVIPDYRAPNCIRLAISP
                     LATSYLEVFEGFERIRDYTASGQYKDIDLTNIKVS"
     gene            complement(1315..2070)
                     /locus_tag="AC16Y1_05245"
     CDS             complement(1315..2070)
                     /locus_tag="AC16Y1_05245"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013287972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5831898.1"
                     /translation="MTRQRTVLVTGGSRGIGAAIAQYFAQQGDRIAAHYSANEKLAEQ
                     VVGSLPGTGHIKVKADLRDPAEIKAMVDQVGNEFRQIDILINNAGVFFDHPIESSSYE
                     VWQQAWADTLGVNLIGAANVTWCALQYMPRNGDSKIVNVGSRGAFRGEPVSPAYGASK
                     AAIVAFGQSIAKALAPEGIGVTTLAPGFVDTEMAAELLNGPTGNDIRNQSPFGRVAEP
                     EELAKVIYFLASKDALWASGAVLDFNGASYLRM"
     gene            2167..2562
                     /locus_tag="AC16Y1_05250"
     CDS             2167..2562
                     /locus_tag="AC16Y1_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013929214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR5831899.1"
                     /translation="MKKLLFAVLGVVSATIFLTACSTDSAAQKVSPKEFSEVIADPSI
                     VILDVRTPQEFAEGHIAGAINIDFQSANFEQEISKLDASKTYAVYCRSSSRSGDAVKV
                     MAKAGFTNLYDMDGGTIEWQAAGGQLVAN"
     gene            2592..2765
                     /locus_tag="AC16Y1_05255"
     CDS             2592..2765
                     /locus_tag="AC16Y1_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831900.1"
                     /translation="MCSRVNCRKCGKVTYSGCGQHLDQVFAGVPHNKRCDCANKQSSS
                     SDKTSLLGKIFGR"
BASE COUNT          791 a          639 c          567 g          812 t
ORIGIN      
        1 ttatgagact ttgatgttag ttaaatcgat atctttatat tgcccggacg ctgtgtaatc
       61 gcgaatacgt tcaaagcctt caaaaacctc aagatatgaa gtcgctaatg gagagattgc
      121 taatcgtatg caattaggtg ctcgatagtc aggtataact ttcatttcat cccgtaagcc
      181 acgagcaatt tttgcgccat ctggatgata gaggctgatg tgtccaccac gctgtttaga
      241 atcgcgcgga gtaactaact catagccaag ctcaacaagc cattgttcgt gaaggtcaat
      301 cataagttga gttccagtcg aagctttgtg attaattgca gaaatagatg cttcttcaat
      361 cattgaaaaa ccttgatcaa tacaaagcaa tccgattatt gatgggctag caatttgaaa
      421 gccgcgtatt ccagcaatct gctcaaactt tgagcccatc agaaattgat ctcgctgagc
      481 aaaccatcct tgaattggca tttttaaatc ttgttgaatc gatttactta aataaagcca
      541 agctggcgca cccggcccag agtttccata tttataagta cagccaactg caatctcaac
      601 accatctcgg tcaaattgca tatcaacagc tccgacggca tggctggcat cccaaaccat
      661 cagtgcgccg tgcgatctaa ctaaatcagt taattcgctt aagttgtgta atgcgcccga
      721 tcgatatgca acaacagaga ggctgactac tgcaacgtcc tggtttaaat aaggagctag
      781 aatctcagca gtaactaact cattgctaaa tacatcgcca acaatatgtg cttttaaatc
      841 atttgggatt attactaagt tcatccctaa ctcgtcggca ataccttgca tgatgtagcg
      901 atcagttgga aaatttgctt cgtctgtgat gattgttttg cgattcggcc tggcatttat
      961 tgcagctcgg atcgctcgat aaaaattaac gctagtcgta tccatcgcca gtgtttgccc
     1021 agaagccgcg cctaatgcag ctcgtcctat taagtcaccg gtggtttgag ctttatcaat
     1081 ccaatctgcc cagccatcaa cgattttcgc gccccaaccg ttcattaaaa agtcatttat
     1141 tagttcaaca gttttcttgg gcaagcgacc aagtgagttt ccatctagat aacaaactgt
     1201 ttcatcttgg acgacaaatt gatttcggta tttgctaaga gcatcttttg cgtctagatc
     1261 gagtgcaaat tctcgagtga tcatgaattt aatcgtattc ctttgttgga ttcgctacat
     1321 ccgcaagtat gaagcgccat tgaaatcaag tactgctcca ctagcccaaa gtgcatcctt
     1381 tgaagctaag aaataaatta ccttggctaa ttcttctggc tcagccactc gaccaaaagg
     1441 actttgattt cgaatgtcat ttcccgtagg gccattcaat aattcagcgg ccatttcggt
     1501 atcaacaaac ccaggtgcca aagttgttac acctattccc tcaggagcaa gtgccttagc
     1561 aattgactga ccaaatgcaa ctattgcagc tttactagct ccgtatgctg ggctaactgg
     1621 ttcgccacga aatgccccac gtgatccaac attgacgatc ttggagtcac catttcgtgg
     1681 catatattgc agcgcacacc atgtgacatt tgcagcacca attaagttaa ctccaagcgt
     1741 gtccgcccaa gcttgttgcc atacttcata acttgaggat tcaatcggat gatcaaagaa
     1801 gaccccagca ttattgatca atatgtctat ctgcctaaat tcattgccaa cttgatcaac
     1861 cattgcttta atttcagcag gatcacgtaa atcggcttta actttaatgt gtccagtgcc
     1921 tggcaatgat ccgacaactt gttcagctaa cttttcattc gcactgtaat gggctgcaat
     1981 tcgatcgcct tgctgcgcga aatattgcgc aatcgcagca cctattcctc ttgagccacc
     2041 ggttactaaa acagttcttt gcctagtcat gaaataagcg tagcgagggc tttaatcctt
     2101 atttgatcct cagttttgta tacccctggg ggtatgctag gattctaaga aaccgaagga
     2161 atctaaatga aaaaattact ttttgctgta cttggagtcg tttcggcgac tattttcctc
     2221 accgcttgct ctacagatag tgcagcgcag aaagtttcac ctaaagaatt ttcagaagtt
     2281 attgctgatc catcaattgt catacttgac gttcggacac ctcaggaatt tgccgagggc
     2341 cacatagctg gcgcaatcaa cattgatttt caaagcgcta acttcgagca ggaaatatca
     2401 aaattagatg catcaaagac ttatgccgtt tactgccgta gtagtagccg ctcaggagat
     2461 gcagtcaaag taatggctaa agcaggtttc accaacttgt atgacatgga tggtggaacc
     2521 atcgaatggc aagctgccgg tgggcaacta gtcgcaaact aatttttaaa actcataaaa
     2581 taggagactt aatgtgtagt cgagttaatt gccgcaaatg cggaaaagtt acttacagtg
     2641 gttgcggtca acatcttgat caagtttttg ctggcgtgcc acacaataaa cgttgcgatt
     2701 gcgctaacaa acaatcaagc tctagtgata agacgtcact tcttggaaag atttttggtc
     2761 gttaattatg caagctactt gtctatcggc gttgctggct tggccagca
//
LOCUS       DBNHVN010000280         1387 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1167226, whole genome shotgun sequence.
ACCESSION   DBNHVN010000280 DBNHVN010000000
VERSION     DBNHVN010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1167226"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /gene="manB"
                     /locus_tag="AC16Y1_05260"
     CDS             <1..1025
                     /gene="manB"
                     /locus_tag="AC16Y1_05260"
                     /EC_number="2.7.7.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018559711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004475 - mannose-1-phosphate
                     guanylyltransferase (GTP) activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HIR5831901.1"
                     /translation="TPKPMLPVAGYSCTEHQIARAREAGVTRIVLGTSYKAEVFKDAF
                     GAGEKLGVELIYAVEQSPLGTGGAIRNAAKHLTCGPDDPVIVFNGDVLTGLNISGLVN
                     KWKDANADVALYLTKVEDPRAYGLVPTDSQGNVTAFLEKPSSPEEIITDQINAGCYVF
                     RRSIIDQIPSDRPVSVERETFPELLTKKAKVIGVVDEGYWLDLGTPLAFAKGSADLVL
                     GNAPSPLVSGRTGKSIVENSAKVDSSAQVFAGTYVGQNVLIEANVVISGSVIFDNVTI
                     KTGAQIINSIISTGAFIGENSNVNGTVVAEKAEVGANNELTSGARVFPNVKISDGAIR
                     FSSDRS"
     gene            complement(1022..>1387)
                     /locus_tag="AC16Y1_05265"
     CDS             complement(1022..>1387)
                     /locus_tag="AC16Y1_05265"
                     /inference="COORDINATES: protein motif:HMM:NF021287.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HIR5831902.1"
                     /translation="SQSKMPAIGYAAHVPHYLATTDYPKAAVALLDQIALSTGLVIPR
                     DELRTAAIAMDEDLDRQIQAVSENVEVVSSLETQHDNLMQSRHELTADGNLVSGDELA
                     AQFEKFLAEQNQKNEGEVD"
BASE COUNT          417 a          268 c          304 g          398 t
ORIGIN      
        1 ctaccccaaa acctatgctg ccagtggctg gctactcttg cactgaacat caaattgctc
       61 gagccagaga agcaggagtc acacgcattg ttcttggaac ttcttacaaa gccgaagttt
      121 ttaaagatgc gtttggtgct ggagaaaaac ttggagtcga attaatttat gcggttgagc
      181 aaagccccct tggaacaggt ggggcaattc gaaatgcggc aaagcattta acttgtggtc
      241 cggatgatcc agtaattgtt tttaacggtg atgtattaac tggcctgaac atttctgggt
      301 tagttaataa gtggaaagat gcaaatgcag acgttgcact ttatttaact aaagtcgaag
      361 atccacgagc gtatggttta gtgccaactg attcgcaagg taatgtcaca gcgtttctag
      421 aaaaaccaag ttctcccgaa gagataatta ctgaccagat aaatgccgga tgctatgtat
      481 ttcgacgttc aataattgat caaatcccta gcgatcgtcc agtatcggtt gagcgcgaaa
      541 cttttccaga actacttacc aaaaaagcaa aagtaatcgg agtagtagac gaaggttact
      601 ggttagacct gggaacgcct ttagcatttg ccaaaggttc cgcagattta gttttaggaa
      661 atgcgccctc gcctttagtt tctggccgaa ccggaaaatc aatcgttgag aattcggcaa
      721 aagtagattc atcagcacaa gtttttgccg gaacttatgt tggtcagaat gtattaattg
      781 aagcaaacgt tgtaataagt ggttcggtaa tttttgataa cgtcacaatt aagacaggtg
      841 ctcaaataat taacagcatt atttcaacgg gtgcattcat aggtgaaaat tcgaatgtta
      901 atggaactgt agttgctgaa aaagcagaag ttggcgcgaa taacgaatta actagcggtg
      961 cacgcgtatt tccaaacgta aaaatatctg acggtgcaat tagattttcg tcagaccgaa
     1021 gttaatcaac ttcaccttca tttttttgat tttgttcagc taagaatttt tcaaattgag
     1081 cggccagttc atcgccactt actaaattgc catcagccgt aagttcgtgg cgagactgca
     1141 tcaaattgtc atgttgagtc tcaagacttg aaacaacctc aacattttca gaaacagcct
     1201 gaatttgtcg atctaaatct tcatccatcg caatagcagc agttcgtaat tcatctctag
     1261 gaattactag tcctgttgaa agtgcaattt gatctagtag cgcaaccgca gcttttggat
     1321 agtcagtagt agctaaataa tgcgggacat gagctgcata gccgatcgct ggcatttttg
     1381 attgact
//
LOCUS       DBNHVN010000281         1409 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1391700, whole genome shotgun sequence.
ACCESSION   DBNHVN010000281 DBNHVN010000000
VERSION     DBNHVN010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1391700"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1348)
                     /locus_tag="AC16Y1_05270"
     CDS             complement(<1..1348)
                     /locus_tag="AC16Y1_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024754.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR5831903.1"
                     /translation="MDSFPADFPISVNPEKLANVFNLTGNRRSGENILTAANLLSSQL
                     REIHPIISPLSFAKPELGPGEIVVGLHKTFADEVSWIADQIAALDMSKLKWKNIAVLI
                     RNKKNIGDFVKALEERNIPVQVADPGDLVDLPEVREVISYLEVIADPTANTALTRILA
                     SPRFAIGARDLAILGRAANELVDQENSGSFDARFELATAQVDRVERACLLDALELVAK
                     SELPYSVQARERMSELSIELRDLRRHASDSVTDLINRIVRTTGLGVQTLINQQGSHQT
                     GCDRISALLDLAGSYVSLDGEMSLRAFVTFLKDAERFDENSTADVSALDNAVTVMSVH
                     KSKGLEFSVVALPSVTKEVFPSTTKLKHWPKSPTELPKELKPTNSYSQAPTFPINRVP
                     RGVDYEKFKKGASTDREIDERRLAYVAVTRAEQTLIASSSWWGPTQKISRGPSDFLV"
BASE COUNT          381 a          318 c          289 g          421 t
ORIGIN      
        1 caaccagaaa atcacttgga cctcgtgaaa ttttttgggt tggaccccac caggagctgc
       61 tagcgataag tgtctgctct gctcttgtta cggcgacata agcaagtcgg cgttcgtcta
      121 tctcacgatc agtgctggca ccttttttaa atttttcata gtcaacgcca cgaggtactc
      181 ggttaatcgg aaatgtgggt gcttgcgagt acgaattggt cggctttaat tccttcggta
      241 actcagttgg agatttaggc caatgtttaa gttttgttgt tgatggaaaa acttcttttg
      301 taacactagg tagggcaact actgaaaact ctaggccttt tgatttgtgt actgacataa
      361 ctgtgactgc attatctaga gcacttacat ctgcagttga gttctcatca aaacgctctg
      421 catcctttaa gaatgtgaca aatgcccgca aagacatttc gccatcaagg cttacgtatg
      481 atccggctaa atcaagaagc gctgaaattc gatcgcatcc agtttggtgt gatccttgct
      541 gattgatcaa ggtttggacg cctaagcctg tcgttcgaac aatccgattg attaaatcag
      601 tgactgaatc acttgcatgc ctacgcaagt cacgaagctc aatcgaaagt tcgctcattc
      661 gttcgcgtgc ttgaactgaa tacggcaatt cactttttgc aactaactca agtgcatcaa
      721 gcagacatgc tcgctcaact cggtcaactt gggcagtagc aagttcaaag cgagcatcaa
      781 atgatcccga attttcttgg tcaactagtt cgttggcggc ccgacccaaa attgccagat
      841 ctcttgctcc aattgcaaat cttggactag ccagaatccg agttaacgcc gtgttagcag
      901 tcggatccgc aattacttct agataactta ttacttcacg aacttcaggt aaatcaacta
      961 agtcacctgg atcggcaact tgaaccggaa tgtttcgttc ttcgagtgcc ttaacaaagt
     1021 ctccgatgtt ctttttatta cgaattaata ccgcgatgtt tttccacttt aatttcgaca
     1081 tatctaaagc ggctatttga tcagcaatcc aactaacttc atcagcaaat gttttgtgta
     1141 agccaactac tatttctcct gggccaagtt ctggctttgc aaaagacaat ggcgaaataa
     1201 tcggatgaat ttctcttagt tgagaactca atagatttgc tgcagtcaaa atgttttcac
     1261 cagatcgacg attgccggtc agattaaata cattcgctaa cttttctgga ttaactgaaa
     1321 ttggaaaatc tgctggaaat gaatccatgt ttgtaatttc tgcaccgcgc cagccataaa
     1381 ttgcctgaca cggatctccg acggcagtt
//
LOCUS       DBNHVN010000282         2220 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_55711, whole genome shotgun sequence.
ACCESSION   DBNHVN010000282 DBNHVN010000000
VERSION     DBNHVN010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_55711"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="AC16Y1_05275"
     CDS             complement(<1..880)
                     /locus_tag="AC16Y1_05275"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5831904.1"
                     /translation="MRPNYSGFGYAFLGVLIFSFTLPMVKVSLPGFSPWTLTFGRAAI
                     AGMLALIFLRLAKVPVPDRSLWKYLIITASGIVVGFPVLTTLALQYTTSAHAAVIVAG
                     LPLVTAVFAVLRVKENVPFLFWIAALIGTFTLVIYAWLNGGNEGVSLIADLMLLGAVL
                     AAAIGYAEGAILVKVMPGWQVVSWCVVLFLPISIPVGAMSFYLGRDTHVVTPVTVGAF
                     LFTALGSMYFGFFAWYRGLSELGVAKGSQVQLLQALLTLAWSALLLDEIVTVRTLIAA
                     SIVILCVAVTQRIRVQK"
     gene            946..1680
                     /locus_tag="AC16Y1_05280"
     CDS             946..1680
                     /locus_tag="AC16Y1_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR5831905.1"
                     /translation="MRATSGATWEQTRSEIVRNALSIGIAVATYGVSFGALGTATGLT
                     IAQTCALSVLMFTGGSQFALVSTLASGGTGVAGALAAILLGTRNTAYSMRMTPLLNTK
                     GPLKFLASHLTIDESTAMGLAQDEKSFSGRATRLAFWTTGLSVFVLWNLATLVGALAA
                     TSIGDPKTLGLDAAIGAGLFALVYPQIQNQKHLVVALGGALVAISLASFVTPGVPIIS
                     ASIVAFIGGYIFTNRAANQNKPEVQK"
     gene            1677..1994
                     /locus_tag="AC16Y1_05285"
     CDS             1677..1994
                     /locus_tag="AC16Y1_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010551385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HIR5831906.1"
                     /translation="MSALWITILTASFACYLLKVLGIVIPKTFLERPLVSEITRLLPI
                     ALLSAIVAIQTLGQGQGLALDARLPALGASLIALRLKAPFVVVVVLAAVVAAVLRYFG
                     FAA"
     gene            complement(1973..>2220)
                     /locus_tag="AC16Y1_05290"
     CDS             complement(1973..>2220)
                     /locus_tag="AC16Y1_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831907.1"
                     /translation="FGKLSYLMGQAIEEIYLPVKELSEEQAREFLDVAIARAKEKMDS
                     WQSWYSVALAYDLLKERKLARQAMQYSIELFQAAKPK"
BASE COUNT          634 a          504 c          488 g          594 t
ORIGIN      
        1 ctttttgaac gcgtattcgt tgagtaaccg cgacacacaa aatcacaata cttgccgcga
       61 ttaaagttcg gaccgtaaca atttcgtcaa gcaaaagtgc ggaccaagcc agagttaaaa
      121 gtgcctgaag taattggact tgtgatcctt ttgcgactcc aagttcacta agcccgcggt
      181 accaagcgaa gaaaccaaaa tacattgaac ccaaggctgt aaataagaag gcgccaactg
      241 tcactggagt tacgacgtgc gtatcccgac ctaggtaaaa actcattgcg ccaactggaa
      301 ttgagatagg taaaaataaa actacacacc aagacacaac ttgccagccc ggcattactt
      361 taaccaagat ggcaccttcg gcatatccaa tcgcagctgc cagcaccgca cccagaagca
      421 tcaaatcagc aataagactg acgccttcat ttccaccgtt taaccaggca taaatcacca
      481 gcgtgaatgt tccgataaga gctgcaatcc aaaaaaggaa gggaacattt tctttaactc
      541 taagtacggc aaatactgca gtaaccaaag gtaggccagc aacaattacc gcagcatgag
      601 ctgaagttgt gtactgcaaa gcaagtgtgg ttaaaactgg aaagccgaca actattccgc
      661 ttgccgtgat tattaaatac ttccaaaggg acctatctgg aacgggcact ttagccaagc
      721 gtaagaatat aagtgcgagc atcccagcta ttgcagcgcg gccgaaagtc aaagtccaag
      781 gagaaaatcc aggtagcgat accttcacca taggtagtgt gaaagaaaaa atcagcacac
      841 ctaaaaaggc ataaccaaaa cctgagtaat taggacgcac agcatttaga ttactggtgc
      901 caagctcgca aaagattaag ttgaagtgac gataaggtta aattcatgcg tgcaactagc
      961 ggagcgactt gggaacagac tcgctctgaa atagtcagaa acgcattaag tatcggaatt
     1021 gcagttgcaa cttatggcgt ttcgtttggt gcacttggta ctgctactgg tttaacgatt
     1081 gcgcagactt gtgctctttc agttttgatg ttcactggtg gtagccagtt tgctcttgtc
     1141 tcaacactgg caagtggtgg taccggagtt gctggcgctc ttgcggcgat cttattagga
     1201 actcgaaaca ctgcttattc gatgcgcatg actcccctgt tgaatacaaa aggcccgttg
     1261 aaattcttgg ccagccacct aacaattgac gagtcaacgg caatgggctt agcgcaagat
     1321 gaaaagtcat tttctggtag agccacccgg cttgctttct ggacaactgg cttatcagtt
     1381 tttgtccttt ggaacttagc cactctggta ggcgcactgg ctgcaacttc gattggtgat
     1441 ccaaaaacac ttggattaga tgcggcaatc ggtgccgggt tattcgcttt ggtctatcca
     1501 caaatccaaa atcaaaaaca tctggtcgta gctctcggtg gcgctttagt ggcaattagt
     1561 ttggctagtt ttgtgactcc tggtgttcca attatttctg cttcaatagt tgcattcatt
     1621 ggtggttaca tttttaccaa tcgtgctgca aatcaaaata aacctgaggt tcaaaaatga
     1681 gtgcactttg gatcaccata ctaacggcaa gttttgcatg ttacttgcta aaagttttgg
     1741 gcatagttat tcctaaaact tttctcgagc gaccactagt tagcgaaatc acccgattac
     1801 ttccgattgc acttttgtca gcaattgtgg cgattcaaac acttggccaa ggacaaggat
     1861 tggctctaga cgccaggctg ccagctcttg gggcgtcact tattgcgctt cgattaaaag
     1921 caccgtttgt ggttgtcgta gttctagctg cagttgtcgc agctgtactt cgttactttg
     1981 gctttgctgc ctgaaataat tcgattgaat attgcattgc ttgacgagct agttttcgct
     2041 ctttaagtaa gtcataggct aaagcaactg aataccagga ttgccacgag tccatctttt
     2101 ctttagcccg cgcaatagca acgtccaaaa attcacgtgc ttgttcctcg gataattctt
     2161 taaccggtag gtaaatttct tcaatggctt gacccattaa ataacttaat ttgccaaatt
//
LOCUS       DBNHVN010000283         2224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1243520, whole genome shotgun sequence.
ACCESSION   DBNHVN010000283 DBNHVN010000000
VERSION     DBNHVN010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1243520"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..786)
                     /locus_tag="AC16Y1_05295"
     CDS             complement(10..786)
                     /locus_tag="AC16Y1_05295"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR5831908.1"
                     /translation="MGNSFLADTSSVQSVDRALSILEILGEKGEVGVTEIAAELDIHK
                     STASRLLSALLSRGLVEQLSDRGKYRLGMGLVRLAGTVSSNLEPVASSRSVTRAIADQ
                     YGETVNIAVLNDNKVLYVDQVAGSHIMTMTSWLGQVVPIHCTATGKVLVAWLDDKQRK
                     ATFPTKFQKLAKNTITSAAEFEKELAKVRERGYAIANEEMDDGFTAVAAPIRDSHLDV
                     VAALVVSGPTARMTKLNLDLVGQNLIKAGKKLSGSPTDWR"
     gene            complement(851..>2224)
                     /locus_tag="AC16Y1_05300"
     CDS             complement(851..>2224)
                     /locus_tag="AC16Y1_05300"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR5831909.1"
                     /translation="GPCFGMKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAAHNLLAA
                     MIDNHIYWGNELGIDVRRIAWKRVIDMNDRALREITAALGGPGNGYPREAGFDITVAS
                     EVMAILCLAMDLDDLERRLGNIVIGYTRDKVAITAKQLNANGAMTVLLKDALMPNLVQ
                     TLENNPAFVHGGPFANIAHGCNTVLATDTALRLGDYVVTEAGFGADLGAEKFFDIKCR
                     KAGLEPAAAVIVATIRALKMHGGVAKDDLGKENVAAVEAGLANLGRHIRNVKKFGVPV
                     VVGVNNFTSDTKAEFDAVQKYCADLGVDAVLCTHWAEGSKGTKVLAEKVVALAESGVS
                     QFAPLYGDELSLWDKVKTIATSIYDADDIVADKTVRDQLKAFEDAGYGKFPICMAKTQ
                     YSFSTDPNLKGAPSNHVVAIREVRLSAGAEFIVVVTGDIMTMPGLPKVPAANSIKLDN
                     GLVVGLF"
BASE COUNT          586 a          549 c          479 g          610 t
ORIGIN      
        1 ttacagtcct catcgccaat ccgttgggct tcccgataat ttcttacctg ccttaattag
       61 attctgacca actaaatcta gatttaactt tgtcatccga gccgttggtc cagaaacaac
      121 tagcgcagcc actacatcaa gatgagagtc tcgaattggg gcagccacag cagtgaaccc
      181 atcatccatt tcttcatttg cgattgcgta accacgttct cgaactttag ccagttcttt
      241 ttcaaactct gcagctgaag tgattgtatt tttggctaat ttctgaaact ttgtcgggaa
      301 tgtggcttta cgttgtttgt catcaagcca agcaactaaa acttttccgg tagctgtgca
      361 atgtattggt actacttggc caagccaact agtcattgtc ataatgtgtg aaccggcaac
      421 ttgatcaaca tataaaactt tattgtcatt caaaactgcg atgttaactg tttcaccata
      481 ttgatcggca atagctcgag ttactgaacg agatgaggca acaggttcta agttgctgga
      541 cactgtgccg gctaggcgaa ctaaacccat gcccaagcga tatttaccgc ggtcagaaag
      601 ttgttcaact aaaccgcgcg agagaagtgc tgacaacaat cgtgatgcag ttgatttatg
      661 aatgtctaac tcagcagcaa tttcagttac gccaacttcg cccttttcac ccaaaatttc
      721 caaaatagaa agggcgcgat ctaccgactg gacagatgaa gtatctgcta aaaaactgtt
      781 gcccatgacg caacattatg cgccatgggc aactagtttg tcctgcgttt tgcaggttaa
      841 tttcttagct ctagaaaagg cctacaacta agccgttatc tagtttgatc gaattagcag
      901 ctggaacttt aggtaggcca ggcatagtca taatgtcgcc agttaccacc acgatgaact
      961 cagccccagc actcaaccga acttcgcgga ttgcaactac gtgattcgaa ggagcgccct
     1021 tgaggtttgg atcagttgaa aatgagtact gggttttagc catacaaatc gggaacttgc
     1081 catagcccgc atcttcaaat gcttttagtt gatcgcgcac tgttttgtcc gcaactatgt
     1141 cgtctgcatc gtaaatgctg gtagcgatag ttttaacttt gtcccaaagc gatagttcat
     1201 cgccataaag cggtgcaaac tgcgagactc cagactcagc tagcgccaca actttttcgg
     1261 caagaacttt agtgcctttg ctaccttcag cccagtgcgt acaaagtacc gcgtctacac
     1321 ctaagtcagc acaatacttt tgtactgcat caaattcggc tttggtatcg gaagtgaagt
     1381 tattcacgcc aactacaact ggtacaccga acttcttgac gttacgtata tgccgaccaa
     1441 ggttagctag gcctgcttca acggcagcaa cattttcttt acctaagtca tctttagcaa
     1501 cgccaccatg catctttagt gcacggatag ttgccacgat tacagccgcc gctggctcta
     1561 gaccggcttt gcgacactta atgtcaaaga atttttctgc acctagatca gcaccgaaac
     1621 ctgcttcggt gacgacgtaa tcgccaagac gaagagcagt gtcagttgct aatactgtgt
     1681 tgcagccgtg tgcgatgttg gcaaatggtc cgccatgaac aaatgctgga ttgttctcta
     1741 gggtttggac caagttaggc attagtgcat ctttaagcaa tacggtcata gcaccattag
     1801 catttagttg ctttgcggta atcgcaactt tgtcgcgggt gtaaccaatg acaatgttgc
     1861 caagacgacg ttctaaatca tctagatcca ttgccaaaca aaggatcgcc ataacttctg
     1921 atgccactgt gatgtcgaat ccagcttccc gtggatagcc gttacctgga ccacctaagg
     1981 ccgcagttat ttcgcgtagt gcacgatcat tcatatcaat aacgcgcttc caggctatac
     2041 gccggacatc gatgccgagt tcgttacccc agtaaatgtg gttatcgatc attgctgcta
     2101 gtaagttgtg cgctgcgcca atcgcgtgga agtcaccggt gaaatgcaga ttgatgtctt
     2161 ccatcggtac aacttgggcg tagccgccac cagcagcgcc tcctttcata ccaaaacatg
     2221 gtcc
//
LOCUS       DBNHVN010000284         1155 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_721648, whole genome shotgun sequence.
ACCESSION   DBNHVN010000284 DBNHVN010000000
VERSION     DBNHVN010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_721648"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..1155
                     /locus_tag="AC16Y1_05305"
                     /pseudo
     CDS             95..1155
                     /locus_tag="AC16Y1_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
BASE COUNT          379 a          249 c          240 g          287 t
ORIGIN      
        1 ctactttttg aaaaaataaa ccaaaattaa tcgtaaacaa aattatttac gatcgaaatt
       61 catcgaggga acaacgtgcg ccatttttaa tagattaacg gacgagcgcc ccggcacaaa
      121 agttataacc gacgacattg aaaatgatcg agtaactgat ggccctaaat cagttactta
      181 caaatatgac ggcggtattg cagatttcgt ttcgcacatc aatgcccgga aacagccaat
      241 ccattcaagt gtgattcagt tttttgccga ggataccaag cgtggcatta gcgccgaagt
      301 agccctgcag tggaatagca cttacaccga atcggtttac accttcgcca acaccatcaa
      361 cacaattgag ggtggaacac acgaagaagg tttccgcacc gcacttacct cactggtgaa
      421 taaatacgct cgtgagtgga gcatcttaaa agaaaaagat ccgaacctga gtggcgaaga
      481 tgttcgagaa ggtttagccg caatcatttc cgttaaattg cgagaaccac aattcgaagg
      541 tcaaactaaa accaaacttg gaaataccga agcgaaaact ttcgtgcaaa aagtagttaa
      601 tgagcaactt gctgaatggt ttgcaaagaa tccaagtgaa ggtaaagaca ttgcacgaaa
      661 atcagtacaa gcagcaacag cacgtttagc agcacgtaaa gcacgtgact tggcccgaaa
      721 ccgaaaaggt ttacttggct caagtggtct tcctggaaaa ttaaaagact gttcaagtaa
      781 tgaaccaagt gaatgcgaag tttttattgt tgagggtgac tcagcaggtg gttcagctgg
      841 ccaaggtcgc gatccgcgca cccaagctat tttgccaatc cgtggaaaaa ttttgaatgt
      901 agaaaaaacc agactcgatc gcgttttgca aaactcagaa gttcaagcaa ttatttctgc
      961 attcggaact ggcatccaag atgattttga tatcaacaaa cttcgatatc acaaattagt
     1021 tttaatggct gatgccgatg ttgacggtca gcatatccgc acccttttac taactttcct
     1081 attccgctac atgaaaccac ttgttgatgg tggcttcgtt tacttagctc aaccaccgct
     1141 atacaaaatt aaatg
//
LOCUS       DBNHVN010000285         1659 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1614036, whole genome shotgun sequence.
ACCESSION   DBNHVN010000285 DBNHVN010000000
VERSION     DBNHVN010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1614036"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="AC16Y1_05310"
     CDS             <1..537
                     /locus_tag="AC16Y1_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR5831910.1"
                     /translation="HWQAGPGKKVAVMGLGGLGHMAVKNAVALGAEVTVLSHSENKRE
                     DAIGMGATHFVNTSQAGELEKLNNTFDVIINTVSAELDLHPYIDSLRMWGSLVVVGAP
                     GKPYSIDAGNLMGRQRSLSGSAIGSIKETQEMLDFCGKHNIVSDVEVISADYINTAYN
                     RVVNSDVKYRFVIDAKTF"
     gene            complement(534..1430)
                     /locus_tag="AC16Y1_05315"
     CDS             complement(534..1430)
                     /locus_tag="AC16Y1_05315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HIR5831911.1"
                     /translation="MANKLQQEFGMRFRRIITNSADGVPDWFPQIESGEDFGLFKPSD
                     APWVVHGDLATLIGGIRALLMQVLHPGSLAGVTDHSRYEKDPLGRLAGTTKWLSILTF
                     GSTQAIATESARVNGMHKRVVGQYEPRPGEQKSYAAADEDLLLWVHIAFTDSFLVAHE
                     FYGAKTIPGGSDAYVSLWSKAVIPLGLALAPQTRAELKEVIKSFDSVLRADQKTLNVV
                     KFIKKPPLSRAARFAFAVMFQAAAITIEPKYRKILGLRVLPTWLVIPTAKLLLNGMRL
                     ALGGVSPLEEAALARHKRLISN"
BASE COUNT          472 a          341 c          374 g          472 t
ORIGIN      
        1 cattggcaag ctggtccggg gaaaaaagtt gctgtgatgg ggctgggtgg actaggtcac
       61 atggccgtaa aaaatgcagt tgctttgggc gccgaagtca ctgttcttag ccacagtgaa
      121 aataaacgtg aagatgcgat tggcatgggc gcaactcatt ttgttaatac gtctcaggct
      181 ggtgagttgg aaaaattaaa taacaccttt gatgtaatta tcaacaccgt ttcagccgaa
      241 cttgatttgc atccctacat tgattcattg cggatgtggg gttcactagt cgttgttggt
      301 gcgccaggaa agccttattc aattgatgca ggtaacttaa tggggcggca acgtagcctt
      361 tctggttctg ctataggcag cataaaagaa acgcaagaaa tgttagattt ctgcggtaaa
      421 cacaacatag ttagtgatgt tgaagttatt agcgccgatt acataaatac tgcatacaac
      481 cgagttgtga atagtgatgt caaatatcgt tttgtgattg acgccaaaac tttttagttg
      541 cttatcaatc ttttatgacg agcaagggca gcttcttcaa gcggtgaaac tccaccgagt
      601 gccagccgca taccgtttaa taacaacttt gctgtcggaa taactagcca agttggcaaa
      661 acgcgcaaac ccaaaatctt gcgatatttg ggttcgattg ttatcgcggc tgcttgaaac
      721 ataacggcaa aagcaaatcg agccgctcgt gacaatggtg gttttttaat gaatttaact
      781 acattcaaag ttttctgatc agccctaagc acagaatcga aagactttat tacctctttt
      841 aattcggcgc gagtttgtgg ggctaatgct aaacctagtg ggattactgc cttactccac
      901 aatgaaacgt atgcatcaga tccaccaggg attgttttcg caccataaaa ttcgtgcgca
      961 actaaaaacg aatctgtgaa ggcaatgtga acccaaagta atagatcttc gtctgctgcg
     1021 gcataagatt tttgttcccc cggtcttggt tcatactgcc caactacccg tttgtgcatt
     1081 ccatttacac gtgcagattc ggtagcaata gcttgagttg agccgaaagt aagtatcgat
     1141 aaccatttag ttgttccggc aagtcggccg agcggatctt tttcatatct tgaatgatct
     1201 gtaactccag ctaaacttcc tggatgtagc acctgcataa gaagtgctct tattccaccg
     1261 atcaaagtcg caaggtcacc atgcacaacc catggcgcgt cactaggttt aaataaacca
     1321 aagtcttcac cactttcaat ttgtgggaac caatcaggaa caccatcagc tgaattcgta
     1381 atgatgcgac ggaaacgcat accaaattct tgctgtaatt tgttagccac ttattctcct
     1441 gtaatgcaga ttccaatcta aatggtgttt tgaatttatg gaacttgaaa attacggtac
     1501 ttcttttgca ccgctggctt ataggctaag aagacacttt gcgaggagaa atatgaagat
     1561 tttggtagtc ggcgcgagcg gattaattgg caaaggcgtc gctgacgaat tagcaaagaa
     1621 acatgaagtt attcgtgctt ctcgaaatgg cgatgttca
//
LOCUS       DBNHVN010000286         1258 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1539529, whole genome shotgun sequence.
ACCESSION   DBNHVN010000286 DBNHVN010000000
VERSION     DBNHVN010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1539529"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="AC16Y1_05320"
     CDS             complement(<1..886)
                     /locus_tag="AC16Y1_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5831912.1"
                     /translation="MTTRITHWIDGKPWSGKAERTSAVFNPATGTQTGEVDLASTKVM
                     ADAVAVAKKAGKEWAAASLTKRTSVLFAFRELLNAHKEDLAAIITAQHGKVLSDALGE
                     VTRGQEVVEFACGIPHLLKGSYSESVSTGVDVYSLRQPLGVVGVISPFNFPAMVPMWF
                     FPIAIGAGNSVIVKPSEKDPSAQNFIAELWKKAGLPDGVFNVVHGDKEAVDAMLEHPD
                     IKSISFVGSTPIAKYIYETGTKNGKRVQALGGAKNHMLVLPDADLDLVADAAVNAGFG
                     SAGERCMAISVLVAVEPIA"
     gene            977..>1258
                     /locus_tag="AC16Y1_05325"
     CDS             977..>1258
                     /locus_tag="AC16Y1_05325"
                     /inference="COORDINATES: protein motif:HMM:NF018399.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HIR5831913.1"
                     /translation="MAQIRKTVVTVEEITRESGQTVSPPTVKVTAAAVVTNPLAGKAL
                     VKDLTELELMSAEVTEYLSKKAIAALGAIGLSASDVRGYGKGAIVGTDGE"
BASE COUNT          332 a          306 c          300 g          320 t
ORIGIN      
        1 ctgcaattgg ttctaccgca actaagaccg agatcgccat gcaacgctca ccagcagaac
       61 caaaacctgc atttactgcg gcgtcagcaa ctagatctaa gtcagcatcc ggtagcacta
      121 acatgtggtt tttcgcacca ccaagtgctt gaacccgctt tccgttcttt gtgccagttt
      181 cgtaaatata ttttgcgatc ggcgttgaac ctacgaaaga aatactcttg atgtctggat
      241 gttccaacat cgcatccacg gcctctttgt caccatgaac aacgttgaat acgccatcag
      301 gtaggcctgc ttttttccaa agctcggcaa tgaaattttg tgcacttgga tctttttctg
      361 aaggtttaac gattaccgag tttccggcac cgatcgcgat tggaaagaac cacattggca
      421 ccatcgccgg gaagttgaac ggtgaaatca cgccgactac accgagtggc tggcgaagtg
      481 aatagacatc aactccagtt gaaacggact cgctataaga accctttaat agatgtggaa
      541 taccacaagc aaattcaaca acttcttgac cacgggttac ttcgccaagt gcatccgata
      601 gaactttgcc gtgttgggca gtaatgatgg cggccaggtc ctctttatgc gcatttagta
      661 actcgcggaa tgcgaaaagc actgaagtgc gcttagtaag tgaggcagct gcccattctt
      721 taccagcttt tttcgctact gcgactgcat cggccataac tttggttgat gcaagatcaa
      781 cttcgccagt ttgtgttccg gtagccggat taaatactgc gctagttctt tcggctttac
      841 cgctccacgg cttaccgtct atccagtggg tgatccgagt ggtcatcctg gcactccttt
      901 gttgaatctg ttctctaatc tagtgcccaa atcaatctag ccttgcagta ggttaaggac
      961 taactggagg tagaaaatgg ctcagattag aaagaccgtg gtaacggtcg aggaaatcac
     1021 tcgggaaagt ggccaaacag tcagtccccc aaccgtaaag gtgacagcgg cagctgtcgt
     1081 gactaatccg ctcgcgggca aagctttagt aaaagattta actgaactag aactcatgag
     1141 cgcagaagta actgagtact tatccaagaa agcaattgcc gcactgggag caatcggatt
     1201 aagtgcaagc gatgttcgcg gatatggcaa gggtgccatt gtcggcactg atggtgaa
//
LOCUS       DBNHVN010000287         3293 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1764801, whole genome shotgun sequence.
ACCESSION   DBNHVN010000287 DBNHVN010000000
VERSION     DBNHVN010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1764801"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="AC16Y1_05330"
     CDS             complement(<1..494)
                     /locus_tag="AC16Y1_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831914.1"
                     /translation="MTESKLSTTQLALEKVGGKYALSWPVAILSFLWASNISISYDAT
                     RFNTSRTGWFLASVLAHVILILLAAPIRYKLLPVVDRPSKPILALTVFAVLGVTRSLL
                     MGEFAVYLNLAPTQEYGYRQITGFISITAGLAVTVILVASVAERQKTLSALLAQRQRL
                     TEIK"
     gene            complement(507..1163)
                     /locus_tag="AC16Y1_05335"
     CDS             complement(507..1163)
                     /locus_tag="AC16Y1_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5831915.1"
                     /translation="MEPRRILVVEDDPFLQALIADSLTSAGFEVKVAGNAANAKSFLA
                     DFDPDAAVLDIELGNGPTGIDLAEIIVRQFPHTAIIFLTHLPDPRFVGVDSAAIPNKA
                     AYIRKETITERGALVKIVENTIQNGAAKNKKLRTDLDPNRPLAALSKSQIAVLRSIAQ
                     GLTNAEIAAQRGSTVRAVEHLVRRTFAAANIDGDQTFNARTAAARAYIQAAGLPTSSD
                     "
     gene            complement(1174..1866)
                     /locus_tag="AC16Y1_05340"
     CDS             complement(1174..1866)
                     /locus_tag="AC16Y1_05340"
                     /inference="COORDINATES: protein motif:HMM:NF038065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HIR5831916.1"
                     /translation="MSNLKFRKYAYGIVALSTWTGFGLSLIIETFGLVEQKLYDPPIP
                     ASQFGFYGNYEDGLAGAPERLLDLFSYFTIWSQIVVGIVATLLFLNPNRDGKYFRVLF
                     LDAVLMITVTGVVYNLLLGPNYPPQGLNQISSPIEHTWTPILMVTAFLLTGPRKWITA
                     SIIPKVLVLPIIYVIYTLARGAIINSYPYDFFDVVTYGYTYVITFVGAILAASLIVLS
                     VFWLIDSRIAKR"
     gene            complement(1969..3072)
                     /gene="rlmN"
                     /locus_tag="AC16Y1_05345"
     CDS             complement(1969..3072)
                     /gene="rlmN"
                     /locus_tag="AC16Y1_05345"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR5831917.1"
                     /translation="MPKPGELIFESPRRAKPPQHWSDLTVEQRKEKVIDLGLPGFRAD
                     QISRQWFSRLSNQPETWTDMPEALRNEIKENFFPELMTSIKSLTCDNGTTVKQVWKLF
                     DGVLVESVLMRYPERVTMCISSQAGCGMNCPFCATGQGGLTRNLTTAEIVEQVLAGAR
                     SLARGEITGGVGRVNNLVFMGMGEPMANYSAVIEAIRRLTDDPPAGLGMSARGITVST
                     VGLVPRIKQLADEGLPVTLAISLHAPDDELRDTLVPINTRFKVSEVLDAAWYYAAKTG
                     RRISIEYALIKDINDQAWRADLLGELLSGHLVHVNLIPLNPTPGSKWTASRPEDEREF
                     VRRLKNHGVAVTVRDTRGREIDGACGQLAASAK"
     gene            complement(3133..>3293)
                     /locus_tag="AC16Y1_05350"
     CDS             complement(3133..>3293)
                     /locus_tag="AC16Y1_05350"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR5831918.1"
                     /translation="ATFGDLIESMIKRDLSIKDMGNLLPGHGGVMDRIVSLLVNAFVA
                     WILFGIFL"
BASE COUNT          959 a          741 c          698 g          895 t
ORIGIN      
        1 tgtttaatct cagttaagcg ctgtcgctgc gctaataagg cacttaaagt tttttggcgt
       61 tcagctacag aggcgaccaa aattaccgtc actgctaagc ctgccgtaat tgaaatgaag
      121 ccagtaattt ggcgatatcc atattcttga gttggcgcca aatttaggta aacagcaaat
      181 tcgcccatta gaagagatct agttactccg agaactgcga aaacagtaag ggcaagtatt
      241 ggtttactcg gtcgatcaac tacgggtagt aatttgtatc taataggtgc ggctaacaaa
      301 attaaaataa cgtgtgccaa tactgacgcc aaaaaccatc cggttcttga tgtattgaat
      361 cgagttgcat cgtaactaat cgaaatattt gaagcccaca aaaaagacaa aattgcaact
      421 ggccaactta gggcgtattt accgccgact ttttccaaag ccaattgagt cgtacttaac
      481 ttggattcag tcataacttc aactttttag tcactcgacg ttggcagccc agctgcttgg
      541 atatatgccc tagcggctgc agttcttgca ttaaaagttt gatctccatc aatgtttgcc
      601 gcagcaaaag tgcgtcgcac gagatgctct actgcgcgaa cagttgatcc acgttgcgcc
      661 gctatttctg cattcgttaa tccttgggca atacttcgca gaacagcaat ctgagacttt
      721 gaaagtgccg ccaacggacg attcggatct aagtcggtac gtaatttttt atttttggcg
      781 gctccatttt gaattgtgtt ttcaactatt ttcaccaagg cgccgcgttc cgtaattgtt
      841 tcttttctga tgtaagcagc tttgtttgga atggctgctg aatcaacacc gacaaatctc
      901 ggatctggta agtgggtaag gaaaataatc gcagtatgcg ggaactggcg aacaatgatt
      961 tcagctaaat caattccggt cggtccatta cctaattcaa tgtcgagaac cgctgcatcc
     1021 ggatcaaaat ctgctagaaa cgactttgca tttgctgcat ttccagcgac tttaacctca
     1081 aatccagccg aagttaagct atcggcaatt aaggcttgta aaaaaggatc gtcttcgaca
     1141 acaagtatcc gacgtggctc catgtcccct agtttagcgt ttggctatcc gactatctat
     1201 tagccaaaaa acgctaagca cgatcaggga tgcagccaaa attgccccaa cgaaagttat
     1261 tacgtaggta tagccgtatg taaccacatc aaagaaatcg tatggatagg aattaattat
     1321 cgcgccccga gcaagagtgt aaatcacgta aataattggt agtaccaaaa ctttcggaat
     1381 tattgaggcg gtaatccact tacgtggtcc agttagcaaa aacgcagtaa ccatcagaat
     1441 tggtgtccaa gtatgttcaa ttgggctaga aatctgattc aaaccttgcg gtggataatt
     1501 cggtccaagt aataagttgt agaccacgcc agttacagtg atcatcaaaa ctgcatcaag
     1561 aaatagaact cggaaatact ttccatctcg atttggattt aagaaaagca aggttgctac
     1621 gatgccaact acaatttgcg accagattgt gaagtaactg aataaatcaa gtaagcgctc
     1681 gggagcaccg gccaagccat cttcgtaatt tccataaaag ccaaattgtg atgctggtat
     1741 cggtggatcg taaagttttt gctcaactaa gccgaaggtt tcaataatta gggacagtcc
     1801 gaaaccagtc caagtgctta gggctacgat cccgtaggcg tacttacgaa atttcaagtt
     1861 actcataact gacaagatta gagccaacat tgaagaaaat actggtaatc gttctggcga
     1921 ttccaggctt aactaccttg aatttgcgct caaaatcaag taaacgcgct actttgcgct
     1981 tgcggctagt tggccgcaag cgccatcaat ttcgcgtcca cgggtgtctc gtacagttac
     2041 cgctacccca tggtttttaa ggcgtcgtac aaattcgcgt tcatcctcag gtcgtgatgc
     2101 cgtccacttt gagccaggtg ttggatttaa cggaatcaaa ttaacatgca caagatgtcc
     2161 ggacaataat tcacctagta aatccgcgcg ccaggcttga tcattaatat ctttgattaa
     2221 cgcatactca attgaaatac gtcggccagt ttttgctgcg taataccaag cagcatctag
     2281 aacttcacta actttaaatc gagtattgat tggtaccaag gtgtcacgca actcatcatc
     2341 tggtgcatgc aatgagatcg ccaaagtaac tggcaaccct tcatcggcta gctgtttgat
     2401 gcgcggaact aatccaactg ttgaaactgt gattccgcga gccgacatcc ctaaaccagc
     2461 tggtggatca tcggtaagtc tgcgaatcgc ttcaataacg gccgagtagt tagccatcgg
     2521 ttcgcccata cccataaaaa ccaaattatt tactcgacca actccgccgg ttatttctcc
     2581 acgagccaag gacctagcgc cggcaagaac ttgttcgaca atttctgcag tagttaaatt
     2641 tctagttaat ccgccttggc cggttgcgca aaatgggcag ttcatgccac aaccggcttg
     2701 cgaagaaata cacatggtga cgcgctcggg atagcgcatc aaaacacttt ctaccaaaac
     2761 gccatcgaac agtttccaaa cttgcttaac cgtggtgccg ttatcgcagg taagcgactt
     2821 tatggaagtc attaattcag gaaaaaagtt ttccttaatc tcatttcgta atgcttctgg
     2881 catatcagtc caagtctcag gctggtttga aagacgactg aaccattggc gcgaaatttg
     2941 gtctgctcga aaaccaggca aaccgagatc aataactttt tctttgcgct gttctacggt
     3001 taaatctgac cagtgttgag gtggttttgc gcgtcgaggc gactcgaaga ttaactcacc
     3061 tggctttggc atagtagtta gcctacgtgg agtttttacc taactgtgaa tttgaaggaa
     3121 tttaatcttt gatcaaagaa aaattccaaa aagaatccaa gcaacaaagg cattaaccag
     3181 tagtgaaact attcggtcca taacgccacc gtgacctggc aataaattac ccatatcttt
     3241 aatacttaaa tcacgtttga tcatagattc aattaagtcg ccaaaggtgg ctg
//
LOCUS       DBNHVN010000288         1887 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_2061164, whole genome shotgun sequence.
ACCESSION   DBNHVN010000288 DBNHVN010000000
VERSION     DBNHVN010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_2061164"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="AC16Y1_05355"
     CDS             <1..1143
                     /locus_tag="AC16Y1_05355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005926.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HIR5831919.1"
                     /translation="QLSRAAQDAQKIVQGQPGSAMTHAWLITGPPGSGRSTSALAFAA
                     ALVCPQGGCGECVDCQNALAGTHIDIEHIVPEGVIYTVEDTRALIERAALAPSRSHWH
                     VIVIEDVDRFRAESASTLLKSLEEPPPGTVWLLCAPTSDDVFETIRSRCRQVVLATPS
                     LELIANQLVQRYGIDKKLASFAARAAQGHIGRARALATDEAVRNRRNETLDMPTRVNS
                     VVSCFEMAAQIVANANADTDSIVEPLDLLDENNIRTAYGDGVEGFKGVERQIKSAVKA
                     SEKRAKDRRRRVLSDQYDRVLLDLTGFYRDVLVVQSDAKTELINEEIRTKIEKVATSS
                     SATETLLRIDAISETRDNLLANVNPTSAFESMLVTLRNPQILTSTT"
     gene            1213..>1887
                     /locus_tag="AC16Y1_05360"
     CDS             1213..>1887
                     /locus_tag="AC16Y1_05360"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5831920.1"
                     /translation="MRKYIATVTAMLLVLVSAIGYTTFESGQPNSATVPSANPENPTD
                     LSGYYDQVLTWENCGGGFECTSLSVPLDYDDPYKDAITISVIRLRVSAQPIGSLILNP
                     GGPGVSGIEYARAAEFVVSKVLLDNFDIVGFDPRGVGESSPIECIDDQKTDEYIASDG
                     TPDDQKEIDNSIEILTQFGQDCLTNSPELISNVDTVSAARDVDILRAALGDEKLNWLG
                     KSYGTFL"
BASE COUNT          547 a          388 c          442 g          510 t
ORIGIN      
        1 cagttaagtc gtgctgcgca agatgcgcaa aaaattgttc aaggccaacc gggcagcgct
       61 atgacacatg cttggttgat taccggccca cccggaagtg gtcgaagcac aagcgcatta
      121 gctttcgcag cagccttagt ttgtccccaa ggtggttgcg gtgaatgtgt tgattgtcag
      181 aatgcgctag cgggtacgca catagatatt gaacacatcg tgccagaagg tgttatttac
      241 actgttgaag atactcgggc attaattgaa cgagcagcac ttgccccaag ccgttcacac
      301 tggcatgtaa ttgtgattga agatgttgat cggtttcgcg ctgaatctgc atcgacatta
      361 cttaagagtt tagaagaacc gccccctggc acagtctggt tactttgcgc gccaacaagt
      421 gatgacgttt ttgaaactat ccgcagccgt tgtcgtcagg ttgtgttagc aactccgtca
      481 cttgagttaa tcgcgaatca acttgtccaa cgttatggaa tcgataaaaa gttggcttcg
      541 ttcgcagcta gagcagctca aggacatatt ggccgagcgc gcgcattagc cacagatgaa
      601 gcagtgcgca acaggcgtaa tgaaacttta gatatgccaa ctagggtaaa ttcggttgtt
      661 tcatgttttg aaatggcagc acaaatagtt gctaatgcaa atgcagacac tgactcaatt
      721 gtcgaaccac ttgacttact tgatgaaaac aacattcgaa ctgcatatgg cgatggtgtc
      781 gagggattca aaggagttga acgccaaatt aaatcagctg ttaaagcttc agaaaaaaga
      841 gcaaaagatc gccgccgtcg agtgctgagc gatcaatacg atcgagtgtt actagattta
      901 actggttttt accgcgatgt tttagtggtg caatcagatg caaagacaga attaattaat
      961 gaagaaatta gaaccaaaat tgaaaaagtt gccacttcta gttcagcaac agagacacta
     1021 ttgcgtattg atgccataag tgagactcga gataatcttt tagcgaatgt caatccaact
     1081 tcagcatttg aatcaatgtt ggtgactttg cgcaacccgc aaatactaac cagcactact
     1141 taattccatt taagtcaggt aggcattaga gttttgctaa ccatactttt ctaatcaagg
     1201 tgctgacatt aagtgcgtaa atacattgcc acagtaacgg cgatgttact tgtattagtt
     1261 agtgccattg gttacaccac ttttgagtcc gggcaaccta actcagcaac tgtgccaagt
     1321 gcgaatccag aaaatccaac tgacttatct gggtattacg atcaggtgct cacttgggaa
     1381 aactgcggtg gcggctttga atgtacgagc ctttcggtgc cactcgacta tgacgatccc
     1441 tataaagatg cgatcacaat ttcagtgatt cgacttcggg tgagtgctca accaattgga
     1501 tccctaattt taaatccagg tggacccggt gtcagcggga ttgagtatgc acgagcggca
     1561 gaatttgtag ttagtaaagt tttgttggac aactttgaca ttgtcggttt tgatccacgc
     1621 ggggtcggtg aaagttcacc aattgaatgt atcgatgacc aaaaaactga tgaatacatt
     1681 gccagtgacg gaacacctga cgatcaaaaa gaaatcgata attcaattga aatccttact
     1741 caatttggtc aagattgctt aactaattca cctgagttga tcagcaatgt tgatacggtc
     1801 agcgctgcac gtgacgtaga cattttgcgt gccgcactcg gtgatgaaaa attgaattgg
     1861 cttggaaaaa gttatggcac attttta
//
LOCUS       DBNHVN010000289         1195 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_128405, whole genome shotgun sequence.
ACCESSION   DBNHVN010000289 DBNHVN010000000
VERSION     DBNHVN010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_128405"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1195)
                     /locus_tag="AC16Y1_05365"
     CDS             complement(<1..>1195)
                     /locus_tag="AC16Y1_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010143644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HIR5831921.1"
                     /translation="SAPGGYLVHLAEQVPADLLAVLEMPNVTYLPRTVVNGLYDHGYT
                     VAIERRTDHESSAVSKNKARIRTWHFRSKKIILATGAYQRHLVFANNDRPGVMLSHAA
                     ATYVARDGVKAFKTAVVVSVDNQGYRDAIRLANAGVKVAAVVDIRGKVSRKSKKSSRK
                     AVKKLGIPVIKKSTAINTLGFEGKLLSVVIGDRDGNVSQTLDCDLLAVSGGWTPTVHL
                     ATYVGLKPEWSKNKAAFVLKASDNWVSAEGMLAGKFGGDDEPEVFFGPELSADLAVEA
                     FVDFQRDATLRDVRRAIKAGMQSIEHVKRYTSIGTANDQGKTSGTITMGLIGAALDKA
                     PDEIGSLTFRAPFVGVPFAALAGRERGQLADPVRRTQIHELHLAAKAPMEDVGQWKRP
                     WYFPQG"
BASE COUNT          309 a          294 c          265 g          327 t
ORIGIN      
        1 ccttgcggaa aataccaagg ccgtttccat tggccaacat cttccatagg tgccttggct
       61 gcaagatgta attcatgaat ttgagttcgg cgcactggat ctgccagttg cccgcgctcg
      121 cgaccagcaa gtgcagcaaa tggaactcca acgaatggtg cacggaaagt tagtgatcca
      181 atttcatctg gcgctttatc aagcgctgca ccgatcaacc ccattgttat agtgccacta
      241 gttttacctt gatcgttagc agtgccaatc gaggtgtaac gcttaacgtg ttcaatactc
      301 tgcatacctg ctttgattgc tctgcgtaca tctcgaagag ttgcatcacg ttggaagtca
      361 acaaatgctt caacggctag atctgcggaa agttctggac caaaaaatac ttcgggttca
      421 tcgtcgccgc caaattttcc agccaacata ccttcggcac taacccagtt atcgcttgcc
      481 tttaatacga aagcagcttt atttttagac cactctggct ttagtccgac ataagtcgct
      541 agatgcacag ttggtgtcca gccaccagag acggcgagta aatcacaatc gagagtctgg
      601 ctaacgttgc cgtcacgatc accaatgaca acgcttagca acttgccttc aaatcccaag
      661 gtattaattg cagttgattt tttaatgact ggtatgccaa gttttttaac ggctttacgt
      721 gacgatttct tactctttcg cgaaactttg cctcgaatgt cgacaactgc agccaccttg
      781 actccagcat tagccaagcg aatagcgtcg cgataaccct gattatcaac agatactaca
      841 actgcagttt tgaatgcttt gactccatcg cgggcaacat aagttgctgc agcatgtgac
      901 agcattacgc caggacgatc attgttagca aagactagat gacgttggta ggcacctgtt
      961 gccaagatga ttttcttaga gcggaaatgc caggtgcgga tgcgagcctt atttttactg
     1021 actgcgcttg attcatgatc tgttctacgc tcgatcgcca cggtgtaacc gtgatcatag
     1081 agaccattta caactgtgcg aggtaaataa gttacgtttg gcatttcaag tacggcaagt
     1141 aaatcagctg gtacttgctc ggctaagtga acaaggtatc cgcccggtgc acttt
//
LOCUS       DBNHVN010000290         4163 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_352612, whole genome shotgun sequence.
ACCESSION   DBNHVN010000290 DBNHVN010000000
VERSION     DBNHVN010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_352612"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="AC16Y1_05370"
     CDS             complement(<1..206)
                     /locus_tag="AC16Y1_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HIR5831922.1"
                     /translation="MPVDQQLMDQVSNWLDQDPDATTKKQLSELVAAAQKNDGTALVE
                     LVDAFRAPLEFGTAGLRGALGPGP"
     gene            253..1422
                     /locus_tag="AC16Y1_05375"
     CDS             253..1422
                     /locus_tag="AC16Y1_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR5831923.1"
                     /translation="MKTSQIIDTAPLVLDVGVVLLLAASLGLLARKLGLPAIIGYLFT
                     GLLVSPFTPGYVAGAEQLAILADIGVILLLFEVGIEIDLRRLRTEQRAIFWASPLQIL
                     TGILISIPVFMWFGIPFWGAVLGGLSVAMSSSVVIVNIVRSRRRTTNPSTETALLGWS
                     IMQDITGVAIAAIVLTIIGVGDRPLPIALLGLVGFGVLAYLSSIALPKLLRFVKWEGD
                     LFLIFSVSIGLTLAAIGTVIFGIPMALAAFVAGLALNQGRETDEVRRVLLPFRDLFAV
                     LFFVVIGSLISFNNLDESIPFALAIILLLIFGKTLPAYLITKFSGVNAQPAQLAVGLS
                     QLGEFSFVLGSAALAKGLINGTQFTAVLMVVVLSIVASTLIVRMFKPQQNNNLAI"
     gene            1516..2250
                     /locus_tag="AC16Y1_05380"
     CDS             1516..2250
                     /locus_tag="AC16Y1_05380"
                     /inference="COORDINATES: protein motif:HMM:NF023818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HIR5831924.1"
                     /translation="MGLLDNLEQRLDRIVNGSFSKAFKSEVQPVELGAALQQELDTRA
                     DNVAGQMVVPNVFVIELSQTDHDRLSPYFGTLISELTQLANNYISEQRYRILHRVEIS
                     FNLDSTFETGVFRIKSASQPPANTVPASSSQSASGQSAMNSQIPVAAMSATEIPRLTD
                     ISGQEFPLTKSVTNIGRGVDADIQIADSGASRLHCAIVLGSIVLVRDLGSTNGTTVDG
                     QRITEAKLQDGSIIKLGNTTFTYKSR"
     gene            2254..2724
                     /locus_tag="AC16Y1_05385"
     CDS             2254..2724
                     /locus_tag="AC16Y1_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein FhaB/FipA"
                     /protein_id="HIR5831925.1"
                     /translation="MSELLVSIVRIGFLVALWWFVISVVGVLRRDLQAPRDAKPLTPA
                     RSSNKPSVRPTLRTRKSPNNIVITEGALEGTVIPLGNTPITIGRAADSTIVLDDDFVS
                     THHARLTPSGKQWIIEDLGSTNGTYIDRDRVNTPTVLRPRVSLRIGRTSMELRS"
     gene            2721..3911
                     /locus_tag="AC16Y1_05390"
     CDS             2721..3911
                     /locus_tag="AC16Y1_05390"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR5831926.1"
                     /translation="MTNFALRFAGRSEIGLVRRNNEDSGYASVDIAVVADGMGGHEAG
                     ELASAATVASVVNAAKLATEADEVLAHLSDAVITAGEYIADVVAANRELTGMGTTMTV
                     VAIRGERMAVAHVGDSRAYLLRDGDFQQITKDHTFVQTLVDAGEITKAEAAVHPRRNL
                     MMRAIDGIHAVDVDLSVREAKVGDRLLLCSDGLCGVVAEEEMRIALSATDLTSAVTEL
                     IDSAIAAGAPDNVTVIVADIVPDTIEIDPVVIGAAADSGNQQRLPEVLFPAEKMEKLN
                     PTEVFYEIKKRSRLVPILSTVFVMSIGFAGALFWLANQWFVGVFNPTGEIATFQGIPS
                     VGLYRVVDVAEFKVNQLPNFERTQVEKTIDATTYAEATEILTNLKSVVTNCQLNPQTP
                     GCPG"
     gene            3919..>4163
                     /locus_tag="AC16Y1_05395"
     CDS             3919..>4163
                     /locus_tag="AC16Y1_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006604455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831927.1"
                     /translation="MSQNLRKYRVSTRRSAELALLFGAWLIGVFAWVLVDNATGAGAL
                     AGWTTLIGSGVLLLVAHLVVRNQAPYADPILLPAAALL"
BASE COUNT         1085 a          852 c          972 g         1254 t
ORIGIN      
        1 ttaggtcctg gacctaatgc gccacgcaaa cctgctgttc caaattcaag tggtgctcga
       61 aatgcatcga ctagttcgac taaagcagtg ccatcatttt tttgagctgc ggcgaccaac
      121 tcacttagtt gcttttttgt agttgcatca ggatcttggt caagccaatt tgaaacttga
      181 tccatcaact gttgatctac gggcacaact taagcctacg tccacaatga tttcgacagt
      241 aaactgttat gggtgaagac atcacaaatc atagatactg cacctttggt attagatgtt
      301 ggtgtcgttt tacttttagc ggcctctctt ggtttacttg ctcgcaaact tgggttacct
      361 gcgattatcg gttacttatt caccgggtta ttggtttcac cattcacacc gggttacgtt
      421 gcaggtgctg aacagttagc aattttggct gacatcggtg taatcctgct tttatttgaa
      481 gttggtatcg aaattgattt acgtcgattg cgcacagagc aacgagcaat tttttgggct
      541 tcgccactac aaatacttac tggaatttta attagcattc cagtttttat gtggtttggt
      601 attccatttt ggggcgcagt acttggtggg ttaagtgtgg cgatgtcatc cagtgtggtg
      661 atcgtaaaca ttgtacgtag tcgtcgacgg acaactaacc catcaacaga aactgctttg
      721 cttggctgga gcattatgca agacattacc ggcgtcgcga tagctgcgat tgttttaaca
      781 atcattggcg taggcgatcg accacttcca attgctttac ttggcttagt tggttttggt
      841 gttcttgctt atctttcatc aattgcatta cctaaattac ttcgattcgt taaatgggaa
      901 ggcgacttgt ttttaatttt ttcagtatct attggtttaa ctcttgcggc aataggcaca
      961 gttatatttg gaattccaat ggccttggct gcattcgtgg ctggcttggc attaaatcaa
     1021 ggtcgtgaaa cagatgaagt gcgtcgagtg ctacttccgt tccgagattt attcgctgtc
     1081 ttattttttg tagttatcgg cagtttgatt tcgtttaata atctagatga gtcgattcca
     1141 tttgcacttg caattattct tttgttaatt ttcggcaaaa cgttgcccgc ttatttaatt
     1201 acaaaattca gtggcgtcaa tgctcagccc gcacaacttg ctgttgggtt aagccaactt
     1261 ggtgagtttt cattcgtgtt gggtagtgcc gcattagcaa aaggcttgat taacggaact
     1321 caattcactg cagttttgat ggttgtggtt ttgtcgattg tggcttcaac gttaattgtg
     1381 cggatgttca agccgcagca aaacaacaat ctggcaattt aattcgtaaa tctgcactaa
     1441 ttcaaagtaa ctcgcttatg gattgcccaa gtaaacttgt atttattcaa tgtttcagcg
     1501 attggagtgg tgcaagtggg tttactagac aacctagaac agcgtcttga tcgcatagtt
     1561 aatgggtcgt tctcaaaagc ttttaaatct gaggttcagc cagtcgaact cggggccgca
     1621 ctccagcaag aactagacac cagagccgat aatgttgccg gtcaaatggt ggtccctaat
     1681 gtttttgtaa ttgaactaag tcagactgac catgatcgac tttcacctta ttttggaacc
     1741 ctaatctctg agttaactca attagcaaat aactacattt cagaacagcg ttatcgaatt
     1801 ttgcatcggg ttgaaataag ttttaatttg gattcgactt ttgaaacagg cgtatttcga
     1861 atcaaaagtg ccagccaacc ccctgcaaat actgttcccg cgtcaagtag ccagtcagca
     1921 agtggccagt cagcaatgaa ctcgcaaatt ccagttgctg ccatgagtgc tactgaaatt
     1981 cctaggctaa ccgacatttc cggacaagaa tttccattaa caaaatcagt taccaacatt
     2041 ggccgcggag ttgatgctga tattcagata gctgatagtg gtgcaagtcg actacattgc
     2101 gcgatagtgc ttggttcaat agtcctagtt cgtgatttag gttcaactaa tggcaccaca
     2161 gtggatggcc agcggataac cgaagcaaag cttcaagacg gttcgattat caaactcggt
     2221 aacacaactt tcacgtataa gagcagataa cttatgtccg aattacttgt gagcattgtt
     2281 cggattggtt ttctggttgc cctttggtgg tttgtaatct ctgttgttgg tgttttgcgt
     2341 cgagacttac aagcaccccg cgatgcaaaa cctttaacac cagctcgcag ttccaataaa
     2401 ccaagtgtgc gcccaacgct tcgtacgcgt aaatcaccaa acaacattgt gattactgag
     2461 ggcgcacttg aaggaaccgt tattccactt ggcaatacac caatcactat tggtcgagcc
     2521 gcagactcaa caattgtttt agacgatgac tttgtgagta cacatcacgc gcggttaacg
     2581 ccgagtggca aacaatggat tattgaggat cttggctcaa caaatggtac ttacatcgat
     2641 agagaccgag taaatactcc aaccgtttta cggccacggg ttagcttgcg aattggtcgc
     2701 acaagtatgg aacttcgttc atgaccaact ttgcacttcg ttttgccggg cgttccgaaa
     2761 tcggattagt cagacgaaac aatgaagata gtggctatgc ctcagttgat attgcggttg
     2821 tagctgacgg tatgggcggc cacgaagcgg gtgaattagc tagtgcggcc actgtagcta
     2881 gtgtcgtgaa cgcagcaaaa ctagcaaccg aagcagatga agttttagcg cacttgtctg
     2941 atgcagtaat taccgcaggt gaatacattg cagacgtagt agctgccaat cgcgaattaa
     3001 ccggtatggg cacaactatg actgtagttg caattcgcgg cgaacgaatg gctgtggcac
     3061 acgttggcga ttcacgcgcg tatttacttc gcgatggtga ttttcagcag ataactaaag
     3121 atcatacgtt tgtgcagact ttagttgatg ctggtgaaat cactaaagct gaagcggctg
     3181 tgcatccaag gcgtaactta atgatgcgcg caatagatgg catacatgca gtcgatgttg
     3241 acctttcagt tcgagaagca aaagttggcg atcgactttt gctttgtagc gatggacttt
     3301 gcggtgtggt tgctgaagaa gaaatgcgaa ttgcgcttag tgcaacggat ttaacaagtg
     3361 cggtgacaga gttgattgac agtgcgatag ctgctggcgc gccagataac gtgacagtaa
     3421 ttgttgctga cattgtgcct gacaccatcg aaattgatcc agttgttatt ggcgccgcag
     3481 ccgacagtgg taaccaacag cgattacctg aagttctttt cccggcagaa aaaatggaga
     3541 aactgaatcc gactgaagtt ttttacgaaa ttaaaaaacg aagtcgattg gtgccaatcc
     3601 tgtcaacagt gtttgtaatg tctattggct ttgccggtgc actattttgg ttggctaatc
     3661 aatggttcgt tggagtattt aatccaactg gtgagatcgc tacattccaa ggaattccat
     3721 cagttggact ttatcgagtt gttgatgttg cagaatttaa agtaaatcag ttgcctaatt
     3781 ttgaacgcac tcaagttgaa aaaactattg acgcaaccac ttacgctgaa gcaacagaaa
     3841 tcttaactaa tctaaaaagt gttgtaacta attgccaatt aaatcctcaa acgcctgggt
     3901 gtccggggtg attgaagttt gagtcaaaat ttacgcaagt atcgagtttc aacacggcgg
     3961 tcagcagagt tggcactact tttcggcgct tggttaattg gcgtctttgc ctgggtattg
     4021 gttgacaatg caactggagc tggtgcacta gctggatgga caacattaat tggttcaggt
     4081 gtgttgctgt tagttgcaca tttagtcgtt agaaaccagg caccttatgc agatccgatt
     4141 ttactcccag cagctgcatt gct
//
LOCUS       DBNHVN010000291         1052 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_575322, whole genome shotgun sequence.
ACCESSION   DBNHVN010000291 DBNHVN010000000
VERSION     DBNHVN010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_575322"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="AC16Y1_05400"
     CDS             <1..649
                     /locus_tag="AC16Y1_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831928.1"
                     /translation="FDIEPLHPEVTLGSFLEVELTKTNLITVSNPPFGRNNSLSIPFF
                     NHAAPHSDLIAFIVPRSWRKWSVINKLDRNFQLVRDLDLAINYVDEFGYEINAKDRLR
                     TCVQFWARTSTPRPLIHVEDMGVITKVTPAEADVSLTIFGYNCGTVNEDFPRVKNTTQ
                     MYLKLNHRKALSALKSANFRQFYLNTATTEALSISEINYALNEVIFNDPKLKLVD"
     gene            complement(638..>1052)
                     /locus_tag="AC16Y1_05405"
     CDS             complement(638..>1052)
                     /locus_tag="AC16Y1_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR5831929.1"
                     /translation="KLANEYSTVPHLIFACGRYEGIDARVAQYYKQNIRVDEVSIGDY
                     VLAGGEAAVLVIVESLARLIPGVLGNSESYQDDSFAVGQMEKLIEGPVYTRPPDWRGL
                     TVPEVLMSGDHKKVAQWQLAQAKHKTAMIRPDLVD"
BASE COUNT          306 a          232 c          199 g          315 t
ORIGIN      
        1 ttttgacatt gagccactcc atccagaagt gacccttggc agtttcttgg aagttgagct
       61 aaccaaaacc aatttgatta ccgtaagtaa tccaccattt ggtcgaaata actctctttc
      121 aattcccttt tttaatcatg ctgcaccaca ttcggattta attgctttca ttgttcctcg
      181 atcctggcgt aaatggagcg taattaataa gttggatcgt aattttcaat tagtacggga
      241 tcttgattta gccattaatt atgtagatga attcggatac gaaatcaatg caaaggatcg
      301 actccggacc tgtgttcagt tttgggcacg aacttcgacc ccaagaccgc ttattcatgt
      361 tgaggacatg ggcgttataa ctaaagtaac tccggcagaa gctgacgttt cgttaacaat
      421 ctttggttat aactgcggga cagtaaatga agattttcca agagttaaaa acacgactca
      481 gatgtacttg aagctcaatc atcgcaaagc tttgagtgct ttaaagagcg cgaacttcag
      541 gcagttttac ctgaatactg caacaactga agcgctttca atatctgaaa ttaactacgc
      601 cttgaatgaa gtaattttta acgatccaaa acttaaatta gtcgactaaa tccggtctga
      661 tcattgctgt cttgtgttta gcttgtgcta attgccattg cgcaactttt ttatgatcac
      721 cagacatcaa cacctctgga accgtcagtc cacgccaatc tggtggccgg gtataaaccg
      781 gaccttcaat tagcttttcc atttgtccaa cagcgaagga atcatcttga taactctcgc
      841 tattacccaa tacacctgga atcaatcggg ctaaagattc aactattacc aagactgctg
      901 cttcaccgcc agcaagtacg tagtcaccaa tggaaacttc atcaacgcga atgttttgtt
      961 tgtagtattg cgctactcgg gcatcaatgc cttcataacg accacaggca aaaattagat
     1021 gaggcactgt tgaatattca tttgcaagtt tc
//
LOCUS       DBNHVN010000292         1142 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1987594, whole genome shotgun sequence.
ACCESSION   DBNHVN010000292 DBNHVN010000000
VERSION     DBNHVN010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1987594"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="AC16Y1_05410"
     CDS             complement(<1..673)
                     /locus_tag="AC16Y1_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HIR5831930.1"
                     /translation="MANTQIARAISGYLDHLIVERGLANNSIFSYRRDLRRYEEFCLS
                     NDLREPKQITSQHISNFLVQLRLGNAEHPALSSSSSARTLIAVRGFHAFLAREGLTPT
                     DPARAVKPPSSAKQLPKALTFPEIELLLNAVGVDAKGDLSFLMLRDRALLELLYGTGA
                     RISELINLTVDEVSQLNPKAPILKLLGKGDKQRLVPVGKPALAALDAYLVRSRPVLAK
                     AGKGSP"
     gene            complement(683..>1142)
                     /locus_tag="AC16Y1_05415"
     CDS             complement(683..>1142)
                     /locus_tag="AC16Y1_05415"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013285593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR5831931.1"
                     /translation="SNSFSIESECIAADLVIGAVLVSGAKAPKLVSNKLVSQMKPGSV
                     LVDIAIDQGGCFEDSHATTHADPTFKVHNSVFYCVANMPGSVPNTSTYALTNVTLPYA
                     VALANKGWKQALKDDKALALGLNTHAGKVVYPAVAQAFDMSYEKLESVLA"
BASE COUNT          322 a          267 c          250 g          303 t
ORIGIN      
        1 ctggcgaacc ctttccggct ttggctaaca ctggccggct gcgaactaaa tatgcatcaa
       61 gtgcagcaag tgctggttta ccaactggca ccaaacgctg cttatcgcct ttacctagca
      121 acttaagaat cggggctttt ggattaagtt gcgacacctc atcaacagtt aagttaatta
      181 actcagaaat tcgtgcccca gtgccataga gcaattcaag taacgcccga tcgcgcaaca
      241 ttagaaaact caaatcccct tttgcatcta ccccaacggc atttagcaat aactcaattt
      301 cggggaaagt tagtgcctta ggtaactgct tggcgctact tggtggcttc acagccctag
      361 ctggatcagt tggcgttaaa ccttcgcgag ctaagaaggc gtgaaaaccg cgcacggcaa
      421 taagtgtgcg cgcacttgaa ctactggata gcgctggatg ctcagcatta cctaaccgaa
      481 gttgcaccaa aaaatttgag atgtgttgtg aagtaatctg ttttggctca cgtaaatcgt
      541 tacttaaaca aaattcctca taccgccgta aatctcggcg atatgagaaa attgaattat
      601 tagctaaacc gcgctcaaca attaagtggt ccaagtaacc cgagattgct cgtgcaattt
      661 gggtattggc catacctaaa acttaagcca aaacgctttc tagtttttcg tatgacatgt
      721 caaaagcttg cgcaactgct ggataaacaa ctttgcccgc gtgagtgttt aacccaagtg
      781 ctagagcctt gtcatcctta agggcttgct tccaaccctt attggctagc gcaactgcgt
      841 atggcaaagt cacgttagtt aaggcgtagg ttgatgtatt tggcaccgaa cctggcatgt
      901 ttgctacgca ataaaacact gagttgtgga ctttgaaagt cggatcagca tgtgtagtgg
      961 catgagaatc ttcgaagcaa ccaccttggt caattgcgat atcaacaagc actgaacctg
     1021 gtttcatttg tgaaacaagc ttgttgctaa ctagctttgg cgctttggca ccagacacaa
     1081 gtactgcgcc gattactaag tcagcagcaa tacattcaga ctcaattgag aaactatttg
     1141 aa
//
LOCUS       DBNHVN010000293         2900 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_501818, whole genome shotgun sequence.
ACCESSION   DBNHVN010000293 DBNHVN010000000
VERSION     DBNHVN010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_501818"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="AC16Y1_05420"
     CDS             <1..410
                     /locus_tag="AC16Y1_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR5831932.1"
                     /translation="TDQVKQTLSQYGLALGEAFQLRDDLLGVFGDAETTGKPTGDDLR
                     EGKQTMLIAVAKELTDSTGQKLIESALDNQQLTVDQVNEIQNLLIKSGAQNRIELRIE
                     ELFQTALGAISNLEPAANAALSELAIMATNRAK"
     gene            411..1946
                     /gene="crtI"
                     /locus_tag="AC16Y1_05425"
     CDS             411..1946
                     /gene="crtI"
                     /locus_tag="AC16Y1_05425"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012087050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR5831933.1"
                     /translation="MVSLREWIPGHTQRTVVGPTENVVIVGAGLAGLSAAMRLAGAGR
                     KVTVVERESIPGGRAGRISDRGFEFDTGPTVLTMPDLIADAFDCLNEDMADWLTLEPV
                     KPLYRTFFADGSQMDVHSDTGQMAAEIEKVIGPDEAAGYHKYVDFVSDLYKYEMRNFI
                     DRNIDTPLDLISKDLIKLISIGGFRKLAPKVEQYLKDDRTQRVYSFQAMYAGLSPYDA
                     LAIYAVIAYMDSVAGVFFPKGGMHAVPQAMADAAAKHGVTFKYNTEVTKVRTNGSRAT
                     GVETKDGEFIAADVVILNPDLPIAHTQLLSSNPWTVSRLTYSPSCYLLLAGSNKKYEH
                     IAHHNIHFGKSWSGVFDELINKKTIMSDPSILVTNPSRSDPALAPAGKDIYYVLFPTP
                     NLDAEIDWKTYEPKYRDLVMRTLDQRGYSDFAESVEVLHITTPQDWADRGMERGAPFA
                     AAHSFFQTGPFRPRNTWGENVVFTGSGTQPGVGVPMVLVSGRLAAERVTGKDTKYRSR
                     ALR"
     gene            1980..>2900
                     /gene="mptB"
                     /locus_tag="AC16Y1_05430"
     CDS             1980..>2900
                     /gene="mptB"
                     /locus_tag="AC16Y1_05430"
                     /inference="COORDINATES: protein motif:HMM:NF053209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol phosphomannose-dependent alpha 1,6
                     mannosyltransferase MptB"
                     /protein_id="HIR5831934.1"
                     /translation="MSLVQTTAKKNLFVAVYIWPGFFGSSFLAIGSLGVGWFPLSAQA
                     LEWSWVDYLQTNSLGQSLARAFVVVGAAFLLQAWLMVGIDALHNKITDVRILYVALAA
                     WCAPLVFTPPLFSRDVFSYYMQGKLQLVGFNPYQTGVALVPGWFQSGVDPLWGETPTP
                     YGPAFLLIEKYIAQISADSALSAAIWFRLTSLLGLLILAIAIPYLARQHGVSDISALW
                     LGAMNPLIIMHFVAGAHNDSLMVALVVLALVFGLNKRWILASFVATLAIGIKPVAIVV
                     LPFLVYLAAESNWVSRIRQGIKTGVISSLTL"
BASE COUNT          792 a          610 c          678 g          820 t
ORIGIN      
        1 caactgacca agtcaagcag acactttcgc aatacggact tgcacttggt gaggctttcc
       61 aactacgaga cgacttactt ggagttttcg gcgacgcaga aacaactggc aagccaaccg
      121 gcgacgacct tcgtgaaggt aaacagacta tgttgatcgc agtcgccaaa gaattaaccg
      181 attcaacagg tcaaaaatta attgagtcag cactagacaa tcagcaactt acggttgacc
      241 aagtaaatga gatccaaaat ttattaatta aaagtggcgc gcaaaatcga attgaactgc
      301 gaatcgaaga actttttcaa actgcattag gtgcaataag caatttagag cccgcagcaa
      361 acgctgcgct atcagaactg gcaatcatgg caacgaatcg agcaaaataa atggtttcac
      421 ttcgggaatg gataccggga cacacccaac gcacagttgt tggaccaaca gaaaatgtag
      481 tcattgttgg tgcaggttta gctgggttgt ctgcagcaat gcgcttagct ggtgcgggtc
      541 gcaaggtcac ggttgtagag cgtgagagta ttcctggtgg tcgagctggt cgaataagtg
      601 accgtggttt tgaattcgat accggaccaa ctgttttaac aatgccagat ttaatcgctg
      661 atgcatttga ttgcctaaac gaagacatgg cagattggct aactttagaa ccagttaagc
      721 cgctgtaccg gacttttttt gctgatggaa gtcagatgga cgtgcatagt gacactggtc
      781 agatggctgc agaaattgaa aaagtaattg gcccagatga agcagctggc tatcacaaat
      841 acgttgactt tgtttcagat ctgtacaagt acgaaatgcg caactttatt gatcgcaaca
      901 ttgacactcc actcgattta atttctaaag acttaatcaa attaattagt attggcggtt
      961 ttcgaaagtt agcgccgaaa gtagagcaat acttaaaaga tgaccgaact cagcgggttt
     1021 attctttcca agcaatgtat gccggacttt ctccatacga cgcattggca atatatgcgg
     1081 tcattgctta catggattca gttgccggtg tgttcttccc caaaggtgga atgcatgcgg
     1141 tgccccaagc aatggcagat gcagcagcga aacatggagt tactttcaaa tacaacaccg
     1201 aagttaccaa agttagaaca aatgggtctc gagctacagg tgtggaaaca aaagacggag
     1261 aatttattgc ggcagatgtc gtaattttga acccagatct accaatcgca cacactcaac
     1321 ttctaagtag taacccttgg actgtaagtc ggctaactta ttcgccgtct tgctacctgt
     1381 tgttggccgg ctctaataaa aagtacgagc acattgccca ccacaatatt cactttggca
     1441 aaagctggtc tggtgttttc gatgagttaa tcaacaaaaa aactattatg tccgacccga
     1501 gtattttggt gacaaatcct tcacgtagtg accctgcatt agcaccagct ggcaaggaca
     1561 tttactacgt gctcttccct acgccgaatc ttgatgcaga aattgactgg aaaacttatg
     1621 aacctaagta ccgcgatcta gtaatgcgca cattagatca gcgtggttat agcgactttg
     1681 ccgagtcagt cgaagttcta cacatcacca ccccgcaaga ctgggccgat cgaggaatgg
     1741 agcgcggcgc tccatttgcc gcagcacact catttttcca gaccggacca tttaggccgc
     1801 ggaatacttg gggagaaaac gttgtgttca ctggttcagg aacgcagcca ggtgtcggtg
     1861 taccgatggt tttagtttct ggtcgactag ccgcagagcg tgtaacaggt aaggatacta
     1921 aataccgatc tcgagccctt cgttaactta attttttcca aattaagtaa cctaggactg
     1981 tgtcactggt ccaaactact gcaaaaaaaa atctttttgt tgctgtttat atctggccag
     2041 gattttttgg ttctagtttc ttagctatag gttcgctcgg agttggttgg tttccattga
     2101 gtgcgcaagc actcgaatgg agttgggttg attacttaca aactaattca ttaggtcaat
     2161 ctttagctcg tgcatttgtt gtagtcggtg cagcattctt attgcaagca tggctaatgg
     2221 ttggaattga cgctttacat aacaagatta cagatgtacg cattttgtac gttgcgcttg
     2281 cagcgtggtg tgcaccgtta gttttcactc caccgttatt cagtcgcgat gtttttagct
     2341 actacatgca aggtaaattg cagttagttg gatttaatcc gtatcaaact ggtgttgcgc
     2401 ttgtcccagg ttggtttcaa tctggcgttg atccactttg gggtgagaca cctacaccgt
     2461 acggtccagc atttttgtta attgaaaaat atattgccca gataagtgca gactcagctt
     2521 tgagcgctgc gatttggttt agattgactt cgttactcgg acttttgatt ctggctattg
     2581 caattccata tttagctaga cagcacggag ttagcgacat atcagcattg tggctgggtg
     2641 ctatgaatcc gttaatcatc atgcactttg tggctggcgc acataacgat tcattgatgg
     2701 tggccttagt ggttttagca ctggtatttg gcttgaataa acgctggatt ctggccagct
     2761 ttgttgccac gttagcaatt ggcataaaac cggttgcaat tgttgtttta ccattcttgg
     2821 tttatctggc agccgaaagt aattgggtta gccgaattcg acaaggaatt aaaactggag
     2881 ttatcagttc gctaacccta
//
LOCUS       DBNHVN010000294         1883 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1988375, whole genome shotgun sequence.
ACCESSION   DBNHVN010000294 DBNHVN010000000
VERSION     DBNHVN010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1988375"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1564)
                     /locus_tag="AC16Y1_05435"
     CDS             complement(<1..1564)
                     /locus_tag="AC16Y1_05435"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR5831935.1"
                     /translation="MTQRIAHMRTLIKAKHVYAPGVSEANCLLVDDNLISWIGQLDSF
                     SHLDLAVDQIVDVSDHFVGPGFVDSHVHLSATGAALTGLDLTKARSAKEAIELLRLHL
                     ANTDEEFVIGHGWEDTNWADAFDWNYPTIEQFLGNRKLYLSRIDVHSALVNIPGFQGL
                     VKQEQHHAVRKYVQDNLPSTQRRNQIFAALQHAASQGIVSVHENGGPHVSGEQDFLDV
                     LDIAAQDGLPQVFAYWGSTELAEVKRLNAFGAAGDLTVDGSIGSKTAWLNNPYEHIEE
                     TGTAFLSAQQISDHLVGCTNMGIQGGFHAIGDGALDAVVSGIRLAISQVGIAAFRSAK
                     HRIEHAEMLNPAELDFLSQAGVVLSMQPVFDELWGKKDGLYEQRLGALRASQMNTFNA
                     ICAAGVTLAFSSDSPVTPIDPWRSIKAATDHHTTKHRISARAAFNAHTRGGWRACGDD
                     NNGVIALGLPANLAVWEVDDYTVKIPDERVRAWSTDERSGTPPLPDLSRKLPSCLLTI
                     RNGGAIFDPNNLW"
     gene            complement(1575..>1883)
                     /locus_tag="AC16Y1_05440"
     CDS             complement(1575..>1883)
                     /locus_tag="AC16Y1_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831936.1"
                     /translation="EKTSTDLVARFGSVSKLIAAAKKNDESIKKGTRENLLNSQNYLT
                     AAMEVVKVRTDAKLPNTLEIPQKINNSKELDFMVNDWGLAKQVNRLLKSLKLPVSNSK
                     "
BASE COUNT          492 a          463 c          372 g          556 t
ORIGIN      
        1 gccaaagatt atttggatca aaaattgctc caccatttct aatagtcagt aaacaacttg
       61 gcagctttct cgagagatcc ggtagtggtg gtgtaccgct tctttcgtcg gtcgaccagg
      121 ctctaaccct ttcgtccggt atttttaccg tgtaatcatc aacttcccac acagccagat
      181 ttgccggcaa tcccaatgca attacaccgt tattatcatc accgcaggct cgccaacctc
      241 ctcgcgtatg ggcgttaaat gccgcccgcg cacttattcg atgcttagtg gtgtgatgat
      301 cagttgcagc ctttatcgac cgccaaggat ctatcggagt taccggagaa tcagagctaa
      361 atgccagcgt tacgcccgca gcgcagatgg cgttaaaagt attcatctga ctagccctta
      421 gtgcgcccaa tctttgttca tagagcccat ctttttttcc ccataattca tcaaataccg
      481 gctgcatact aagtacgacg ccagcttgcg aaagaaaatc taattcagca gggtttagca
      541 tttccgcgtg ctcaatccga tgttttgccg acctgaaagc agcgatgcca acttgcgaaa
      601 tcgccaatct gattccagat acaacggcat ctagtgcacc atctccaatg gcatgaaaac
      661 cgccttgaat ccccatgttt gtacaaccaa ctaagtgatc tgaaatctgc tgggcagata
      721 agaatgcagt gccagtttcc tctatgtgtt cataaggatt attgagccaa gccgttttgg
      781 aaccaatcga accatccacg gttaagtccc cagctgcacc aaatgcattt aatcttttga
      841 cttcagccag ttcagtactt ccccaatatg caaacacttg cggtaagcca tcctgcgctg
      901 cgatgtctaa tacgtctaga aaatcttgtt caccagaaac gtgtggtccg ccattttcgt
      961 gcacactaac tattccttga ctagccgcat gttgcagtgc ggcaaaaatt tgatttcgcc
     1021 gctgggtgct tggcaaatta tcttgaacat attttcttac cgcatgatgt tgctcttgct
     1081 tcactagacc ctgaaaacct gggatgttaa ctaacgctga atgaacatct attcggctca
     1141 agtaaagttt tcgattgcca agaaactgtt cgattgtcgg ataattccag tcaaatgcat
     1201 ctgcccagtt cgtgtcttcc cagccatgac caatgacaaa ttcctcatct gtattagcta
     1261 gatgtaatcg aagtagttca atagcctctt tggcacttct agctttagta aggtctaatc
     1321 cagttagtgc ggctccagtg gccgaaaggt gtacgtgaga atcaacaaag cctggcccta
     1381 caaaatggtc cgatacatca acgatttgat caacagctag atctagatga gaaaaactat
     1441 ctagctgccc aatccagctg attaagttgt catctacgag taaacaattt gcctcgctga
     1501 caccaggggc ataaacatgt ttggctttaa ttagtgtgcg catatgtgca attctttgag
     1561 tcaatttgaa atgtctactt agagtttgaa accggtagtt tcaaactttt aagtagccgg
     1621 tttacttgct tagctagtcc ccagtcatta accataaagt ctagttcctt cgaattatta
     1681 atcttctggg gaatttctaa tgtgttaggt aacttggcat ctgttcgcac ctttaccact
     1741 tccatggccg cagttaggta gttttgcgaa tttagcagat tttctctggt acctttttta
     1801 atcgattcat catttttctt ggctgccgct atcaacttgc tcacacttcc aaaccgagca
     1861 actaaatcag tagaagtttt ttc
//
LOCUS       DBNHVN010000295        13417 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1839685, whole genome shotgun sequence.
ACCESSION   DBNHVN010000295 DBNHVN010000000
VERSION     DBNHVN010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 13417)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13417)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13417
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1839685"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="AC16Y1_05445"
     CDS             complement(<1..479)
                     /locus_tag="AC16Y1_05445"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006522 - obsolete alanine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR5831937.1"
                     /translation="MTQHHAIADIDLGKLSKNVETLKQKIDRSALMAVVKANAYGHGL
                     VQSAQAAKRGGADWLATALLDESIDLRNAGITGPILCWLNTPDDEFAQCIDKDIDLGV
                     NSLETLNSIASAAKSLGKTARVHIKVDTGLGRNGVTLGDLPDLISALKKSQNDRNVNV
                     "
     gene            complement(484..852)
                     /locus_tag="AC16Y1_05450"
     CDS             complement(484..852)
                     /locus_tag="AC16Y1_05450"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR5831938.1"
                     /translation="MTIIGIGVDVVDLARFAEVIERTPGIVDRLFTKTEQQSAEGHEL
                     PLQSLAGRFAVKEAVAKALKAPAGMHFHDCEVSNGGAPVVKISGTVQSVADDLGVTNW
                     HISISHDGPVAIAYVIAEKV"
     gene            complement(913..2754)
                     /gene="glmS"
                     /locus_tag="AC16Y1_05455"
     CDS             complement(913..2754)
                     /gene="glmS"
                     /locus_tag="AC16Y1_05455"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016575940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR5831939.1"
                     /translation="MCGIVGYVGPQQALTVVIEGLRRLEYRGYDSAGVAVVTDGKIES
                     RKKAGKLANLEADIAANPISKSSTGIGHTRWATHGAPNDVNAHPHLSEDGSIAIIHNG
                     IIENFASLRDKLIAEGVTLRSQTDSEVVAHLLAKQVVTTPNLGDALRAVCQSLTGAFT
                     LVAVHADNPDLVVAARRNSPLVVGRGKGENFLASDVSAFIAHTRNAIELGQDQVVEIT
                     PDSIKVTDFQGKSVDVVEYEVTWDASAAEKGGYDLFMLKEIAEQPKAVADTLLGRIDL
                     TGKVTLSDLNLSDDYLKSIDKIIITACGTAYHSGLIAKYAIEHWTRVPVEVELASEFR
                     YRDPILTAKTLVVAISQSGETMDTLMAMRYAREQGAKVLAVCNTNGSTIPRESDAVIY
                     THAGPEIAVASTKAFLTQIVALYLFAMYLAQTRGTGFGEAANEIALELASMPNKIDLV
                     LDTAQGVKRIAEEINQATSILFLGRSVGFPVALEGALKLKELAYMHAEGFAAGELKHG
                     PIALIEDGLPIFVIVPPVEDELHDKIVSNIQEVRARGAKTIVLAEEGDDSINEFADHI
                     MRLPKCPPLMQPLLATVPLQIFSCEMATVKGYDVDQPRNLAKSVTVE"
     gene            complement(2796..4151)
                     /gene="glmM"
                     /locus_tag="AC16Y1_05460"
     CDS             complement(2796..4151)
                     /gene="glmM"
                     /locus_tag="AC16Y1_05460"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR5831940.1"
                     /translation="MSDRLFGTDGVRGLANGLLTAQLATSLGVAAAQVLAKRGDLVKH
                     NSHRPFAIVGRDPRISGEFLQAAISAGLASAGVDVVDVGVVPTPAVAFLTDQTGADLG
                     VMISASHNAMPDNGIKFFAKGGQKLADDVEDEIEKQLSVKSQLPTGSDVGNIRQDHDL
                     VEKYIEHLLASIDQSLAGLKVVVDGANGSASLVGPETLRRAGVQVITIHCEPDGLNIN
                     DNCGSTHMQDLFGAVAEHGADVGIAHDGDADRFLAVAEDGSFVDGDQILAILAIGRKA
                     KNELVGNTVVATVMSNLGFNIAMRKSEIEVVATAVGDRYVLEAMRENGFTIGGEQSGH
                     VILSEYASTGDGILTALHVLNEMKRSGKSLSDLAQIVTRLPQVLINVKGVDKLKVSNS
                     QLQSAVKDAESELGETGRVLLRPSGTEPLIRVMVEAASQEQAQTIAEKLAKVVQESCS
                     L"
     gene            complement(4156..4662)
                     /gene="rpsI"
                     /locus_tag="AC16Y1_05465"
     CDS             complement(4156..4662)
                     /gene="rpsI"
                     /locus_tag="AC16Y1_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR5831941.1"
                     /translation="MTQVNDAALIEDLDGAQEELTEYTSETPAAAPREPRKAPNKNGG
                     GTGRRKEAVARVRIVPGSGRWVINGRTLENYFPNKVHQQLVNEPFKVLNFDGAYDVIA
                     RINGGGISGQAGALRLGVARCLNEIDVDGNRPDLKKAGLLTRDARVIERKKYGLKKAR
                     KASQYSKR"
     gene            complement(4700..5143)
                     /gene="rplM"
                     /locus_tag="AC16Y1_05470"
     CDS             complement(4700..5143)
                     /gene="rplM"
                     /locus_tag="AC16Y1_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR5831942.1"
                     /translation="MRTFTPKSGEITREWHVIDATDVVLGRLAVQVATLLRGKHKPIF
                     ASHVDTGDFVIVINADKVALTGSKRENKMDYRHSGFPGGLRATNYAELLETNPRRAIE
                     KAVKGMLPHNKLSDQQISKLKVYAGETHPHTAQNPKPFVISQVAQ"
     gene            complement(5328..6188)
                     /gene="truA"
                     /locus_tag="AC16Y1_05475"
     CDS             complement(5328..6188)
                     /gene="truA"
                     /locus_tag="AC16Y1_05475"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR5831943.1"
                     /translation="MNKPLVTNVPEGFIRIRGEVAYDGKNFSGWGLQPDQRTVQGAIE
                     EAFSQIVGGPRTIVQCAGRTDAGVHASGQVMHLDVPNSWHDRLADLAYKVNAILDEDV
                     VIKELLHTDQNFDARFAALSRSYTYFIQEGLRDPLTRERVYQHKRQLDVDAMNSASQV
                     LIGLHDFSVFCRKNEYGTSIRNLQKFNWTRTQDGLIRVDITSDAFCYSMVRALVGAVI
                     SIGEGKRTKEWLAQYLVGGQRDFGVFMAPAHPLTLVHVEYPPASEYASRIEKTLKSRH
                     NEPAQAPDGD"
     gene            complement(6205..6777)
                     /gene="rplQ"
                     /locus_tag="AC16Y1_05480"
     CDS             complement(6205..6777)
                     /gene="rplQ"
                     /locus_tag="AC16Y1_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015883596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR5831944.1"
                     /translation="MPRPAKGARMGGSPAHERLILANLATALFEHGRITTTEARARRL
                     RPVAERLVTIAKKGDLAARRRVLTVIRDKGVVHTLFTEIGPRFEHRQGGYTRIIKIGP
                     RKGDNAPMAVIELVEPLAEAVVREATAATKKAAQTAPAAVVEDVVAPEAIETAEEVAA
                     AEEVEAIEAEVEQNDSEESVEATEDSESEK"
     gene            complement(6822..7901)
                     /locus_tag="AC16Y1_05485"
     CDS             complement(6822..7901)
                     /locus_tag="AC16Y1_05485"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR5831945.1"
                     /translation="MLISVRPTLSEEVISETRSRFVLAPLEPGFGYTLGNSLRRTLLS
                     SIPGAAVTSVKIEGVLHEFTTIEGVREDVTEFILNLKNLVVSSQFDEPVVMYLRKQGA
                     GPVTAADIQPPAGVEIYNPDLHLATLTDKGKLDMELVVEQGRGYISAANNKASGGEIG
                     RIPVDSIYSPVLKVTYKVEATRVEQRTDFDSLVLDVETKGSIAPRDAVASAGSTLVEL
                     FGLARELNVDAEGIEIGPSVTDQFAAEQLNMPVEALDLTVRSYNCLKREGIHTVGELI
                     SRSEADLMDIRNFGAKSIDEVKDKLASMGIALKDSPPGFDPSQYASAYDDDYDEDEDI
                     FKPAGEVASSEESDDDQSFAEDEQL"
     gene            complement(7956..8582)
                     /gene="rpsD"
                     /locus_tag="AC16Y1_05490"
     CDS             complement(7956..8582)
                     /gene="rpsD"
                     /locus_tag="AC16Y1_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011602317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR5831946.1"
                     /translation="MARYTGADCKRCRREKTKLFLKGSKCDSPKCPMESRPYPPGQHG
                     RGRSKESEYLLQKREKQKAARIYGVLEKQFRGYYEEASRKHGKTGENLLRILESRLDN
                     VVYRAGFAKSRDMARQVVRHGHILVNGTKVDIPSFRVSPNDVVEVRKASLELTPFQVA
                     YAEIGERTVPGWLEVIPAGMRILVHSLPAREAIDTQVQEQLIVELYNK"
     gene            complement(8609..9016)
                     /gene="rpsK"
                     /locus_tag="AC16Y1_05495"
     CDS             complement(8609..9016)
                     /gene="rpsK"
                     /locus_tag="AC16Y1_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008747488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR5831947.1"
                     /translation="MPPKKTTAAGAKKIRRKEKKNIAVGQAYIKSTFNNTIVSITDPT
                     GAVISWSSSGQVGFKGSRKSTPFAAQQAAEDAAKRAIEHGMRKVDVFVKGPGSGRETA
                     IRSLQASGLEVGTIQDMTPVPHNGCRPPKRRRV"
     gene            complement(9059..9436)
                     /gene="rpsM"
                     /locus_tag="AC16Y1_05500"
     CDS             complement(9059..9436)
                     /gene="rpsM"
                     /locus_tag="AC16Y1_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR5831948.1"
                     /translation="MARLVGVDLPREKRLEIALTYIFGIGRTRADEILNATGISPDLR
                     VKDLTDEQLVPLREYIEANFKIEGDLRREVQADIRRKVEIGSYQGIRHRRGLPVHGQR
                     THTNARTRKGPKKTVAGKKKAAK"
     gene            complement(9593..9706)
                     /gene="rpmJ"
                     /locus_tag="AC16Y1_05505"
     CDS             complement(9593..9706)
                     /gene="rpmJ"
                     /locus_tag="AC16Y1_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HIR5831949.1"
                     /translation="MKVNPSVKPMCDKCKVIRRHGVVMVICENPRHKQRQG"
     gene            complement(9721..9942)
                     /gene="infA"
                     /locus_tag="AC16Y1_05510"
     CDS             complement(9721..9942)
                     /gene="infA"
                     /locus_tag="AC16Y1_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR5831950.1"
                     /translation="MAKKDGAIELEGTITEALPNAMFRVELENGHKVLAHISGKMRMH
                     YIRILPDDKVVVELSPYDLTRGRIVYRYK"
     gene            complement(10113..10943)
                     /gene="map"
                     /locus_tag="AC16Y1_05515"
     CDS             complement(10113..10943)
                     /gene="map"
                     /locus_tag="AC16Y1_05515"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR5831951.1"
                     /translation="MIFGRKEKFEIKTFAQFLQMRKAGLVVADTLARIATEAGPGVTT
                     ADLDQIAREELKRFGATPSFLGYHGYPAVICSSVNDEVVHAIPNERPLKDGDILSVDF
                     GAIVDGWHGDSAVTIEIGTKIPAQTKLSEVTRDAMWEGIAKTLADNYLTDISHAVEKT
                     IRTAGNYGILEEYTGHGIGTKMHMEPSVPNYGLPGNGPQLQVGMALAIEPMVTLGARH
                     VHTLSDNWTVVTDDGSRASHWEHTVAITEEGPWVLTAHDGGAEYFTAHGISSPAQQLS
                     "
     gene            complement(10956..11513)
                     /locus_tag="AC16Y1_05520"
     CDS             complement(10956..11513)
                     /locus_tag="AC16Y1_05520"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009396398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR5831952.1"
                     /translation="MRLLIMGPPGAGKGTQAEFIAKEFGIPHVSTGDLFRANLAQGTA
                     LGQEAKKYMDAGEYVPDSVTNGMVRDRLKQADAQAGFLLDGYPRTVAQVAELDGMMAG
                     KVLNKVIVLTADTNEIVLRLMNRAKEQGRADDTEEVIRRRLEVYEEQTAPLINLYKSR
                     GLIVEVDGLGTVNEVTQRIKQAISA"
     gene            complement(11513..12805)
                     /gene="secY"
                     /locus_tag="AC16Y1_05525"
     CDS             complement(11513..12805)
                     /gene="secY"
                     /locus_tag="AC16Y1_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014674383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR5831953.1"
                     /translation="MLSAFAAAFRTPDLRKKILFTLGIITIFRLGSVVPTPGVDYGSI
                     QTCIALVQDNSIYGLINLFSGGALLQLSIFALGIMPYITASIIIQLLTVVIPRLEMLK
                     KEGQSGTAKITQYTRYLTIGLAVLQSTTILTLARTPGQLFQGCNEQLIPDQSIQVLLT
                     MVIVMTAGTSIIMWLGELVTDRGVGNGMSILIFTSIISTFPGQLASIFRIDGSLKFII
                     TLLIGLVTIAGVVFVEQAQRRIPVQYAKRMVGRRSYGGTSTYLPIKVNQAGVIPVIFA
                     SSLLYLPLLIVQLSGSQSDWAQWLATNFDSGRSLYLLTNFLLILFFAYFYISITFNPV
                     DVADDMKKYGGFIPGIRAGKPTSDYLAYVINRITFPGALYLALIATIPYIAFALLGVG
                     QRFIFGGTSLLIMVGVGLDTVKQIESQLQQRNYEGFLK"
     gene            complement(12854..13294)
                     /gene="rplO"
                     /locus_tag="AC16Y1_05530"
     CDS             complement(12854..13294)
                     /gene="rplO"
                     /locus_tag="AC16Y1_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR5831954.1"
                     /translation="MTLKVHHLRPNPGSKTAKTRVGRGEGSKGKTAGRGTKGTKARYQ
                     VPERFEGGQMPLHMRTPKLKGFKNAFRTEYQAVNVSTINALFPKGGAVSVADLVKAGA
                     VRKGELVKVLGQGEISVAVQVSAHAFSESAKTKIAAAGGSANQI"
     gene            complement(13297..>13417)
                     /gene="rpmD"
                     /locus_tag="AC16Y1_05535"
     CDS             complement(13297..>13417)
                     /gene="rpmD"
                     /locus_tag="AC16Y1_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR5831955.1"
                     /translation="TLRALGLKRIGDTVVKEDRPEFRGMVNTVRHLVSVEEVK"
BASE COUNT         3468 a         3180 c         2892 g         3877 t
ORIGIN      
        1 acatttacgt ttctatcatt ttgtgatttt tttaatgcac tgattaaatc tggcaaatcg
       61 ccaagtgtta ccccgttacg gccaagtcca gtatcaacct tgatatgaac gcgagctgtt
      121 ttaccaagag attttgcagc tgatgcgatc gagttaagag tttcgagaga atttactccc
      181 aagtcaatat ctttatcaat acattgcgcg aattcatcat cgggcgtatt tagccaacaa
      241 agtattggac cggtaatccc agcatttcgt aaatcaattg actcatctag cagagccgtg
      301 gctaaccaat cggctccacc gcgctttgct gcttgtgcac tttgtaccaa gccatgaccg
      361 taggcgtttg ctttaacaac ggccataagt gccgaacgat ctattttttg ttttaatgtt
      421 tctacatttt ttgaaagttt gcctagatca atgtccgcta tcgcgtgatg ttgagtcatt
      481 tagttaaacc ttctcggcaa ttacgtatgc gatggccaca ggtccatcat gactaattga
      541 aatatgccag ttagttacac ctaagtcatc agctaccgac tgtactgtcc ccgaaatttt
      601 cacaacagga gcgccgccat tgctgacttc gcaatcgtga aaatgcatac ctgcaggtgc
      661 tttaagtgcc tttgccactg cttctttaac tgcaaatctg ccagcgagag attgaagggg
      721 cagttcgtga ccctctgcac tttgttgttc ggtctttgtg aataggcgat caactattcc
      781 gggtgtgcgc tcaatcactt cggcgaagcg ggcaagatca actacatcca cgccgatgcc
      841 aatgattgtc atgtattaac tttgccctta atttagcgaa ttatgaaatt aagcggattt
      901 ttcaataggt aactattcga ccgtaacgct cttggctaga tttcgcggct ggtccacgtc
      961 atagcctttc acggtcgcca tttcacaact aaatatttgg agtggaacag ttgctaaaag
     1021 tggttgcatt agcggtgggc acttgggtaa gcgcataatg tgatcagcga attcgttaat
     1081 tgaatcatcg ccttcttcag caagcacaat cgtttttgcg ccacgggctc gaacttcttg
     1141 aatgttagaa acaattttgt cgtgcaattc atcctcgacc ggaggaacaa taacgaaaat
     1201 cggtagccca tcttcaatca atgcgattgg tccatgcttg agttctccag ccgcaaaacc
     1261 ttccgcatgc atataagcaa gctctttgag ttttagcgcg ccttccagtg cgaccggaaa
     1321 gccaacactt cgtccaagga acaatattga cgttgcttga tttatttctt cagcaattct
     1381 tttaactcct tgagcggtat ctagcactag atcaatcttg tttggcatgc tggctaattc
     1441 gagggcgatt tcatttgccg cttcaccaaa acctgtgcca cgagtttgtg ccaaatacat
     1501 cgcgaataaa tagagcgcaa ctatttgagt taagaatgct ttggtcgatg ccaccgctat
     1561 ttctggacca gcatgcgtat aaataaccgc atcactttca cgtggaatag tggaaccgtt
     1621 cgtgttgcat acggctaaaa cttttgctcc ttgttctctg gcgtaacgca ttgccatcaa
     1681 ggtatccata gtttcgccag attgactgat cgcaacaact aaagtcttcg cggtcaaaat
     1741 tggatcgcga tatctaaact cactggctaa ctcgacttca actggaactc gagtccaatg
     1801 ttcaattgca tatttggcaa ttaatcctga atggtaagcc gttccacaag cagtaatgat
     1861 tattttgtca atacttttta aataatcatc acttaagttc aaatctgaca aagtgacttt
     1921 gccggttaaa tcaatgcgac ccaataacgt atctgccaca gcttttggtt gttcggcaat
     1981 ttctttgagc ataaaaaggt cgtagccgcc tttttcggca gcagatgcat cccaagtcac
     2041 ttcgtactca acgacgtcca cggacttacc ttgaaaatca gttactttga ttgagtctgg
     2101 agttatttcg acaacttggt cttggcctag ctcaattgca tttcgcgtgt gagcaataaa
     2161 agcagataca tccgaggcta aaaagttttc acctttcccg cggccaacta ccaacggtga
     2221 atttcttctc gcagcaacta ctaaatctgg attatcggca tgcacggcaa ctaaagtgaa
     2281 tgcacctgtt aaggattggc aaactgcgcg taatgcatca ccaagatttg gcgtagtcac
     2341 aacttgtttt gcaagtaagt gcgccactac ttctgagtcg gtttggcttc gcaaggtcac
     2401 gccttcggcg atcagtttgt ccctaagtga agcgaagttt tcgatgattc cgttatggat
     2461 gattgcaatt gagccatctt cacttaagtg tggatgcgca tttacgtcat taggtgcacc
     2521 atgagtagcc cagcgtgtat ggccgatgcc cgttgaagat tttgagattg gattagctgc
     2581 gatgtcagct tcgaggtttg ctaattttcc agctttttta cgcgactcaa tttttccgtc
     2641 ggtaaccacg gcaacacctg cggagtcata acctctgtat tcaagacgac gtaaaccttc
     2701 aataactacg gttaaggctt gttgcggtcc aacatatccg acaattccgc acatgaatta
     2761 cagtctagcc cttagccatt ttggatcggg tgagcctaaa gactgcagga ttcttgaaca
     2821 acttttgcaa gtttttcggc aatagtttgt gcttgttctt gagatgcggc ttcaaccatc
     2881 acgcgaatta gtggctcagt tccactgggc cgaagtaaaa ctcgaccagt ttcgcctaat
     2941 tcactttcag catctttcac agcagattgc aattgcgaat tagaaacctt aagtttgtcg
     3001 acccccttga cattaatcaa aacttgaggg agtcgagtaa cgatttgtgc taaatcactt
     3061 agagattttc cggagcgctt catttcattg agcacatgaa gggccgtcaa aatcccatca
     3121 cctgtcgagg catattcact caaaatcaca tggccacttt gttcgccacc aattgtgaaa
     3181 ccgttctcgc gcatggcttc aagtacgtaa cgatcgccta ctgcagtagc tactacttct
     3241 atttctgact ttcgcatggc tatgttgaag cccaaattag acatcacagt ggccactact
     3301 gtattgccaa ctaattcatt ttttgctttg cgaccaatag ccaaaattgc caaaatttga
     3361 tcgccatcga caaaggagcc atcctctgca actgctaaaa atcgatccgc atcgccatca
     3421 tgtgcaatcc caacatctgc gccatgttca gcaactgcgc caaataagtc ttgcatgtgt
     3481 gttgagccgc aattgtcgtt aatgttcagc ccatcaggct cacaatgaat agtaataact
     3541 tgcacgccgg ctcgacgcag agtttctggg ccaactagcg aagccgaacc atttgctccg
     3601 tcaactacaa cttttaggcc tgctaatgac tgatcgatgc tggctaataa gtgctcaatg
     3661 tacttttcaa ctagatcatg atcttggcga atgtttccaa catcgctacc cgtcggaagt
     3721 tggcttttga ctgaaagttg tttttcgatt tcatcttcaa catcatctgc gagtttttga
     3781 ccgcccttag caaaaaactt aattccgtta tctggcattg cgttgtggct cgctgaaatc
     3841 attacgccaa gatctgcgcc agtttgatca gtaagaaatg caactgctgg agtcggaact
     3901 acgccaacat ctacaacatc gactccggca ctggctaagc cggctgaaat agcggcttgt
     3961 aaaaattcac cgctgatccg aggatctcga cccacaattg cgaacggccg atgtgaatta
     4021 tgttttacta ggtcgccacg tttagccaaa acttgtgccg cagcaacgcc tagagaagtt
     4081 gctaactgag cagtaagtaa tccgttcgct aacccgcgaa cgccatcggt gccaaaaagg
     4141 cgatccgaca tttgcttaac gctttgagta ctgtgaagcc ttgcgtgctt tctttaggcc
     4201 gtacttctta cgttcaatta cacgcgcatc acgagttagt aatccagcct tcttaagatc
     4261 tgggcgatta ccatcaacat caatttcatt taaacaacga gcaacgccga gacgtaatgc
     4321 gccagcttgg cccgagattc cgccaccatt gatccgagcg attacatcgt atgccccatc
     4381 aaagttaagt accttgaatg gttcattaac taattgttgg tgaactttat tcgggaaata
     4441 attttctagt gtgcgaccat tgattaccca acgacctgag cctggcacaa tgcgcacgcg
     4501 ggctactgcc tctttacgac gaccagtgcc gccgccattt ttgttcggcg ccttacgcgg
     4561 ttcgcgtgga gcagctgctg gtgtttcaga agtgtattca gttaactctt cttgggcacc
     4621 atcaaggtct tcgattaatg ctgcgtcgtt tacttgtgtc atgtcgccta gtttcctttt
     4681 ctaaatcttt cgatggcgct tactgagcga cctgtgaaat tacaaatggt tttggattct
     4741 gcgcagtgtg tgggtgtgtt tcaccggcat aaacttttag tttgctaatc tgctggtcac
     4801 ttagcttgtt atgcggaagc attcccttaa cggctttttc aatagcacgg cgcggattag
     4861 tttccaataa ttctgcataa tttgtagcac gtaaaccgcc tggaaaacca gagtgacggt
     4921 aatccatttt gttttcacgc tttgaaccag ttagcgctac tttgtcggca ttgatcacga
     4981 taacgaaatc acctgtgtca acgtgtgaag caaaaattgg cttgtgcttg ccacgaagta
     5041 atgtggctac ctgcacagca agacgtccta atacaacatc agttgcatcg ataacgtgcc
     5101 attcgcgagt gatctcgcct gactttggtg taaacgtgcg cacagttagc cttctttatt
     5161 acttagtggt tttaagtatc gggtcgcaat ttgcttattt ttaaagcgtt gcgacttcca
     5221 aagatacctg tcatatgggt tagtggtcaa aatcgattcg ggaaaaaaac ccagtcaatg
     5281 atgggttttg agaagtaagt ggcccagaaa cgtgggtttt attcgcacta atccccgtct
     5341 ggggcttgcg ctggctcgtt atgcctggat tttaaagttt tttcgatccg actggcgtac
     5401 tccgacgcgg gtgggtactc aacgtgcacc aaagtcagtg gatgtgccgg tgccatgaaa
     5461 actccaaaat ctcgttgacc tcccaccagg tactgcgcaa gccactcctt cgtccgttta
     5521 ccctcgccaa tggaaataac cgcacctact aaagcgcgca ccattgaata gcaaaatgca
     5581 tctgaagtta tatcaactct gatcaaacca tcttgagttc ttgtccaatt gaatttttgt
     5641 aaatttctaa tggaagttcc atattcattt ttccgacaaa aaacagaaaa atcgtgtagc
     5701 ccaatcaaaa cctgactagc actattcatg gcatcaacat ctaactggcg cttatgttgg
     5761 taaacccgtt cacgggttaa cggatcgcgc aaaccctctt gaataaagta tgtgtaactg
     5821 cgagacagtg ccgcaaatcg agcatcaaaa ttctgatctg tatggagcaa ttcctttata
     5881 actacatctt catccaaaat cgcatttact ttatatgcca agtcagctaa acggtcatgc
     5941 catgaattag gtacatctaa atgcatcact tgacctgatg catgtacacc ggcatctgtg
     6001 cggccagcgc attgaactat tgttcgaggg ccaccaacaa tttgactaaa tgcttcttca
     6061 attgcacctt ggacagttcg ttgatccggt tgtaatcccc atcctgaaaa attttttccg
     6121 tcataggcga cctcgccccg aatacgaata aacccctcag gcacatttgt gacgaggggt
     6181 ttattcattt taatctaaaa gtatttactt ttcagattcg ctatcttcgg tagcttcaac
     6241 tgattcttct gaatcatttt gctctacttc agcttctatc gcttcaacct cttcggctgc
     6301 tgcaacttct tctgcagttt caatagcttc tggagcaaca acatcttcaa ctacggcagc
     6361 tggtgctgtt tgcgcagctt tcttagttgc agcagttgct tcgcgaacta cggcttcagc
     6421 aagtggttca actaattcga taactgccat tggggcgttg tcacctttac gtggaccgat
     6481 cttgataatg cgggtgtagc caccttggcg atgttcaaaa cgtggaccaa tttcggtaaa
     6541 gagtgtgtgc accacacctt tatcccgaat aacagttagc acgcgacgac gagctgctaa
     6601 gtcacctttc ttagcaatgg tgactaggcg ctcggcaact ggacgaagac gtcttgcacg
     6661 agcttcggta gttgtaatgc gaccatgttc gaataacgca gttgcaaggt ttgccaaaat
     6721 taaacgctca tgcgctgggc ttccacccat gcgcgcgcct ttagctggtc ttggcatctt
     6781 tctctcctgt caattctctt taccaacaga tgagcaacta attaaagttg ctcatcctcg
     6841 gcaaaactct ggtcgtcgtc agattcttcg ctactagcta cttcaccagc aggcttgaag
     6901 atatcttcat cttcgtcata atcatcgtcg taagcacttg cgtactgact tggatcaaat
     6961 cccggagggc tgtccttaag cgcaataccc attgaagcta atttgtcttt aacttcatca
     7021 atcgacttag cgccaaaatt acggatgtcc attaggtctg cttcagagcg tgagatcaat
     7081 tcaccaacag tgtgaatacc ttcgcgcttt aagcagttgt atgaacgaac tgtcagatct
     7141 agtgcttcaa ctggcatgtt tagttgttca gcagcgaact ggtcggttac tgacggccca
     7201 atctcgatac cttcagcatc aacgtttagt tcacgagcaa gaccgaataa ttcaacaagt
     7261 gttgaacctg cagaagcaac tgcatcgcgt ggtgcaattg aacccttggt ttcaacatct
     7321 agaactaaag agtcaaagtc ggtgcgctgt tccacgcgag ttgcttcaac tttgtaagtc
     7381 acctttagaa ctggtgagta aattgagtca actggaatac ggccgatttc gccaccactt
     7441 gccttgttgt tagcagctga gatgtagcca cgaccttgtt caacaactag ttccatatct
     7501 aacttgccct tgtcggtcaa ggtagctaaa tgtagatctg ggttgtaaat ttcaacacca
     7561 gctggtggtt gaatgtcggc tgcagttact gggcctgcac cttgtttacg taggtacata
     7621 acaactggtt catcaaactg tgaagaaacc acaagattct tcaagttaag gatgaactca
     7681 gttacatctt ctctgacgcc ttcaattgtg gtgaattcat gaagaactcc ctcaatcttg
     7741 acgctagtta ctgcagcacc tgggatagat gaaagcagcg tacgacgcag tgaattacct
     7801 aatgtgtatc caaagcctgg ctccaatggt gcgagcacaa agcgtgaacg ggtttcggaa
     7861 attacttctt ccgagagggt tggacgtact gagatcagca ctttttcttc cttccagcga
     7921 caaccactat ttgaagtcgc agtttgtggg gatgtttact tgttgtaaag ttcgacgatc
     7981 aactgttcct ggacctgtgt atcaattgct tcacgggcag gaagtgagtg gaccaaaata
     8041 cgcatgccag ctggaattac ttctagccaa ccagggacag tgcgttcgcc aatttcggca
     8101 taagcaactt ggaacggagt tagttctagc gatgctttac gtacttcgac tacatcgtta
     8161 gggctaacgc ggaaagacgg aatgtctacc tttgtgccgt taaccaagat gtggccgtgg
     8221 cgaacaactt ggcgagccat atcgcgactc tttgcgaatc cagcgcggta gacaacattg
     8281 tctagacgtg attcaagaat gcgaagtagg ttttcgccag tctttccatg cttacggctt
     8341 gcttcttcgt aataaccacg gaattgcttt tcgaggacac catagatgcg cgcagctttt
     8401 tgcttttcgc gcttctgaag taagtactca gattccttgg accggccacg accatgttgg
     8461 cctggagggt agggacgact ttccattggg cacttcggtg aatcacactt tgaacccttt
     8521 aggaataatt tggttttttc gcgacggcaa cgcttgcagt cagcaccggt ataacgagcc
     8581 attatttaat ctcctattac ttcttcaatt aaacgcggcg gcgcttcggt gggcggcagc
     8641 cattatgagg gactggggtc atgtcttgga tagttccaac ttctagacca cttgcctgca
     8701 gtgaacgaat cgcagtttcg cgacctgaac ctgggccttt aacaaataca tctactttgc
     8761 gcataccgtg ttcgattgca cgcttagctg catcttctgc tgcttgttgt gctgcgaaag
     8821 gtgtgctctt gcgactacct ttaaagccaa cttgacctga ggatgaccat gagatcactg
     8881 cgccagttgg atcggtaatc gaaacaattg tgttgttgaa tgtgctcttg atgtaagcct
     8941 ggcctacagc aatattcttt ttctctttgc ggcggatttt tttagcaccc gcagcggtag
     9001 ttttttttgg aggcattgaa ttcctttttc taataattag ggaagaagtt cggcgacttt
     9061 acttagccgc tttcttcttt ccggccacgg tcttctttgg acctttacgg gtacgggcat
     9121 tggtgtgggt gcgctgtcca tgaacaggta gaccacgacg atgacggata ccttggtaag
     9181 aaccgatctc aaccttgcgg cggatgtcgg cttgtacctc gcgacgaagg tcaccttcaa
     9241 ttttgaagtt ggcttctatg tattcgcgca acggaactaa ttgttcgtcc gttaggtcct
     9301 tgactcgaag atctgggctg ataccggttg cattcagaat ttcatctgct cgcgtacggc
     9361 caatgccgaa gatataggta agtgcgatct ctagacgctt ttcgcgtggg aggtcaacac
     9421 caacaaggcg tgccatcagg cgttactcat ttctgttctt ggtaaaggtc tcacacgttc
     9481 accattcctt gccgcctgac aaggtctcca gcctttactc acctggaggt gtcgttccgt
     9541 cttcacgcac gcgtaaagtt cgggatcggg tgatcgcctt atttcttgtt tactaacctt
     9601 gacgttgttt atgacgtggg ttttcgcaaa tgaccataac tacaccgtga cgacgaatga
     9661 ctttgcactt gtcacacatt ggtttaacgg agggattaac cttcattttt tagttcctga
     9721 tcacttgtaa cggtaaacaa ttcgtccacg agttaagtcg tatggagata gctccacaac
     9781 aactttgtca tccggaagaa tacgaatgta gtgcatccgc atttttcctg agatgtgtgc
     9841 aagaacttta tgtccgttct caagttctac tcgaaacatt gcattcggta gtgcttcagt
     9901 gatggtgcct tctaattcga tggcgccgtc ttttttggcc atatagtttt tgtactccca
     9961 tggatttttg gccaattacc gtttagtaac tgggccgaat cacgatgtta cggcaaaacc
    10021 caccaaaaca tgaaatccgc tcagagcgga cccattgaaa taaggctttt cgaccaggaa
    10081 tattcctcga ttagaagtgc gtataaatac agtcaagaaa gttgctgggc tgggctggaa
    10141 atgccatgtg cagtgaaata ttctgcgcca ccatcatgag ctgttagcac ccatgggccc
    10201 tcttcagtta tcgcaactgt atgttcccaa tgactggctc ttgaaccgtc gtcagtcacc
    10261 actgtccagt tatcgctaag agtatgaaca tgacgtgcgc caagtgtgac cataggttca
    10321 atagccagcg ccattccaac ttgaagttgc ggtccatttc caggcagtcc gtaattaggt
    10381 actgaaggtt ccatgtgcat ttttgtgccg attccatggc ccgtatattc ctcaagaatt
    10441 ccgtaattac cagcagttct aattgttttt tcgactgcat ggctaatgtc agttaagtaa
    10501 ttgtccgcaa gagtttttgc gataccttcc cacatggcat cccgagtaac ttcacttaat
    10561 ttagtttgtg ccggaatctt agttccaatt tcaatcgtga ctgcactgtc gccgtgccaa
    10621 ccatcaacga ttgcgccaaa gtcaacggac agaatgtcac cgtcttttaa tggtctttca
    10681 tttggaattg catgaactac ttcatcattc actgaagaac aaataactgc cgggtaaccg
    10741 tgatagccca aaaatgatgg cgtagcaccg aatctcttta actcctcgcg cgcaatttga
    10801 tcaagatcgg cggtagtaac gccaggtccg gcttccgtcg caatccgcgc caacgtgtct
    10861 gcaacaacta atcctgcttt tcgcatttga agaaattgtg caaaagtttt aatctcaaac
    10921 ttttctttac gaccaaatat catttgtaaa taaaactaag ctgatatcgc ttgtttgatt
    10981 cgttgtgtaa cttcgttaac agtgcctaat ccatcgactt ctactattaa gccgcggctt
    11041 ttgtataaat taattaacgg tgcagtttgt tcttcataaa cttcaagtct gcgacgaata
    11101 acttcttcag tgtcatccgc gcgaccttgc tcttttgccc gattcattaa ccgtaaaact
    11161 atttcattag tatccgcagt tagcacaatg actttattaa ggactttgcc ggccatcatt
    11221 ccatcgagtt ctgcgacttg cgcaaccgtg cgcggatatc catcaagtaa aaatccagct
    11281 tgagcatcag cctgctttag ccgatcacga accattccgt ttgtaactga atctggaaca
    11341 tattcgccag catccatgta tttttttgct tcttggccaa gtgcggtgcc ttgcgctaag
    11401 ttcgcgcgga ataaatctcc ggtagaaaca tgcggaattc caaactcttt tgcgataaat
    11461 tctgcttggg ttcctttgcc ggctccgggt ggccccatga tcagcaagcg cattacttta
    11521 agaaaccttc ataattacgc tgttgtagtt ggctttcaat ttgcttaact gtatccaagc
    11581 caacaccgac cataatcagc aagctagttc caccaaaaat aaatctctgg ccaacgccta
    11641 acaaggcaaa tgcaatgtag ggaattgtgg cgattaaggc aaggtaaagt gcgcccggaa
    11701 aagtaattcg gttaatgacg taagccaaat aatctgaagt cggtttacct gcccgaatac
    11761 ctggaataaa tccaccatat ttcttcatat cgtcagctac atcaactggg ttgaatgtaa
    11821 tcgaaatgta gaagtacgca aaaaatagaa ttaataaaaa gttagttagt aaataaagac
    11881 tacgaccaga atcaaagttc gtggccaacc attgtgccca atcggattga gagcctgata
    11941 gttgaacgat taacaatggt aagtacaaaa gtgatgaagc gaaaataact ggaataacgc
    12001 cagcctggtt taccttgatt ggcaggtatg ttgaagtgcc gccgtaagaa cgacgaccaa
    12061 ccatgcgctt ggcatactgc actggaattc gacgttgtgc ttgttcaacg aatacaacac
    12121 ccgcgatagt aaccaaaccg attaacaagg taattatgaa tttaagtgag ccatcaatcc
    12181 ggaaaattga ggccaactga cctgggaagg ttgaaataat tgaggtgaaa ataagaatcg
    12241 acataccatt accaacaccg cgatcagtta ctaattcacc tagccacata atgatcgagg
    12301 taccagcagt catcacgata accattgtga gaagtacttg aattgactga tctggaatta
    12361 attgttcgtt acagccttga aatagttggc cgggtgtgcg agccaacgtc aaaatagttg
    12421 ttgactgtag aactgccaag ccaatagtta agtaacgggt gtattgcgta atttttgctg
    12481 ttcccgactg accttctttt ttgagcattt caagacgtgg aattactacc gttaacaact
    12541 ggatgatgat gctcgccgtg atgtatggca tgatgccgag tgcaaaaatc gataactgta
    12601 gtaacgcgcc accactaaat aagttgatta gcccataaat actgttatct tgcacaagcg
    12661 caatacaggt ctgaatggat ccgtagtcca ctccaggagt tggaactacg gagcccaaac
    12721 gaaagatcgt aataatgcca agagtaaaca gaattttttt gcgcaagtcc ggagtacgga
    12781 atgctgccgc aaatgcactt aacactgttt tcctcctggc ttaattacta agagtctttt
    12841 tagtaattaa gacttagatc tggttggctg agccaccggc tgcagcgatt tttgtcttag
    12901 ctgattcaga aaatgcatgc gcactcacct gaacagctac cgaaatttcg ccttggccaa
    12961 gtacttttac aagttcgccc ttgcgaactg cgccagcctt aactagatca gccactgata
    13021 ctgcgccacc ctttgggaaa agtgcattaa ttgtggaaac atttactgct tggtattcag
    13081 tacggaatgc attcttgaaa cctttaagtt tcggagtacg catgtgtagt ggcatttgac
    13141 caccttcaaa acgttccgga acttggtaac gagcttttgt accctttgta ccgcgacctg
    13201 cagtcttacc ctttgaacct tcaccacgac cgacacgagt cttcgcagtt tttgaacctg
    13261 ggtttggacg taaatgatga accttaagcg tcatagttac ttaacctcct cgacactaac
    13321 taagtgacga acggtattaa ccatgccacg aaattctggg cgatcttctt tgacaacggt
    13381 gtcaccgatg cgctttaaac caagagcgcg aagtgtt
//
LOCUS       DBNHVN010000296         2536 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1097290, whole genome shotgun sequence.
ACCESSION   DBNHVN010000296 DBNHVN010000000
VERSION     DBNHVN010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1097290"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="AC16Y1_05540"
     CDS             complement(<1..855)
                     /locus_tag="AC16Y1_05540"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004949488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR5831956.1"
                     /translation="MSQISWPLLPAAQQPEWPDSAALEKAQDILQSLPPLVFAGECDE
                     LTEKLGQVANGEAFVLMGGDCAETFAANTADSIRARLKTVLQMAIVLTYGASLPVVKI
                     GRIAGQYGKPRSADLETFNGQSLPSYRGDAVNGIEFSPEARTPDPMRLVQTYHASAAA
                     LNLIRAFTQGGYADLRQVHAWNQDFVSESAPGKRYEQMAAEIDRALSFMTACGADPEE
                     FRRVDFYASHEALLLDYEKPLTRVDSRTGNLYDVSGHFVWIGERTRDLAGAHVDFAAR
                     IENPIGVKV"
     gene            1007..2227
                     /locus_tag="AC16Y1_05545"
     CDS             1007..2227
                     /locus_tag="AC16Y1_05545"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008102225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrophosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HIR5831957.1"
                     /translation="MAVKKVALLTAGGLAPCLSSAVGALIERYSQVAPEVEIIAYRNG
                     YHGLLKGDYLTVTPEIRAKARLLQDLGGSPIGNSRVKLTNVADCVKRGLVKEGQDPQQ
                     VAADQLVKDGVDVLHTIGGDDTNTAAADLAAFLAKNNYGLGVVGLPKTIDNDVYPIAQ
                     SLGAWTAADEGAKYFSNVVSEHTSNPKMLIVHEVMGRHCGWLTAATAVSYRKLLDDQE
                     WLPEFGAARPQRDIHSVYVPELEIDIEAEAKRLSGVMDSLGNVNIFLSEGAGVDAIVE
                     EMRNRGEEIPLDPFGHVKIDRINPGAWFAAQFAPMIGAEKVLVQKSGYFSRSAPANEK
                     DKQLIEDCAHVAVEAALTGVSGLVGMDEDASNVIRACEFSRVAGAKPFNPNQSWFIDL
                     LEAIKQPKGKPTSH"
     gene            complement(2228..>2536)
                     /locus_tag="AC16Y1_05550"
     CDS             complement(2228..>2536)
                     /locus_tag="AC16Y1_05550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR5831958.1"
                     /translation="TRSPDGRLYRGKTGVARMALDAKVKVIPVAMINTEKVQPIGRSR
                     PNFNVDVGLRIGKPLDFSRYYGMEEDRFVLRSITDEIMYNLMELGGQEYVDKYASRKR
                     "
BASE COUNT          699 a          575 c          600 g          662 t
ORIGIN      
        1 aacttttact ccaattggat tttcgatccg ggcagcgaaa tcgacatgtg caccagctaa
       61 atcccgagtg cgttcaccaa tccaaacgaa atgtcccgat acatcataaa gatttccagt
      121 gcgcgaatct acgcgcgtga gtggcttttc gtaatcaagg agtaatgctt cgtggcttgc
      181 ataaaaatct acgcggcgga attcttctgg atctgcaccg caagcagtca taaaagataa
      241 cgcccgatca atttcggccg ccatttgctc atagcgttta cctggcgccg attcactaac
      301 aaagtcttga ttccaagcat ggacttggcg taagtctgca tagccacctt gggtaaaggc
      361 gcgaattaaa ttaagcgcag ctgcgctggc gtgataagtc tgaaccaaac gcattggatc
      421 tggagttcta gcttctggtg aaaactcaat accgttgacc gcatctccgc ggtaagaagg
      481 aaggctttgt ccgttgaaag tttctaaatc agcacttcgc ggtttcccat attgaccggc
      541 tatacgtcca atcttcacaa caggaagtga cgcgccataa gtaagtacaa tcgccatttg
      601 caaaaccgtt tttaatcttg ctcgaattga atctgccgta tttgccgcaa atgtttcggc
      661 acaatctcca cccatgagaa caaatgcttc accgttggcc acctggccga gtttttcggt
      721 taactcatcg cattcgccag caaaaactaa tggcggtaat gactgcagaa tatcttgggc
      781 cttttctaag gcagcactat ctggccactc tggttgttga gcagccggca gtaacggcca
      841 agaaatttgc gacacggctc taggttagtc gggcttcagg tggattacta aatcgagcaa
      901 atcatcattt ggttcgttac accaggtggg cgcactaagt aaggcgcact aagtaaggcg
      961 caccaggtgg acgtattaag taacccacac tgagtagatt tgagatatgg ctgttaaaaa
     1021 agttgcgcta cttaccgcag gtggccttgc cccttgttta tcttctgcag ttggcgcgct
     1081 aattgagcgt tactcgcaag ttgctccaga agttgaaatt attgcgtatc gaaatggcta
     1141 tcacggctta ctgaagggtg actatttaac tgtcacccca gaaatccgcg ctaaggcccg
     1201 cctactccag gatctgggtg gaagcccaat tggaaatagt cgagttaagt taactaacgt
     1261 agccgactgc gttaaacggg gtttagttaa ggaaggccaa gacccccagc aagttgctgc
     1321 cgaccaactt gtaaaagatg gcgtcgacgt acttcacaca atcggcggtg acgacactaa
     1381 tactgcagct gctgatctgg cagctttctt agcgaaaaat aattatggat tgggtgtagt
     1441 tggtctacct aaaacaatag ataacgacgt gtatccaata gcccaatcac tcggtgcatg
     1501 gacagctgcc gatgaaggtg cgaaatattt cagcaatgta gtttcagaac atacttcaaa
     1561 tccaaaaatg ctaattgtgc atgaagtaat gggccgccat tgtgggtggt taactgctgc
     1621 taccgcagtt tcatatcgaa aattattgga tgatcaagaa tggttacctg agtttggtgc
     1681 ggcaagacct caacgagaca ttcatagtgt ctacgtacct gaattagaaa tcgacatcga
     1741 agctgaagca aaaagattaa gtggcgtgat ggactcgctg ggtaatgtga atatttttct
     1801 ttcagagggt gctggtgttg atgcaatagt tgaagagatg cgcaaccgag gcgaagaaat
     1861 accattagat ccgttcggcc acgtaaaaat tgatcgaata aatccaggtg cctggtttgc
     1921 tgcccagttt gcgccaatga ttggcgcgga aaaggtgctc gtccaaaaaa gtggctactt
     1981 ttctcggtca gctccggcta acgaaaaaga taagcaactt attgaagatt gcgcacacgt
     2041 tgcagtagaa gctgcgttga ctggagtgag tggcctagta ggaatggatg aagatgctag
     2101 taacgtcatt cgggcttgtg aattttctcg ggtagcagga gccaagccat tcaatccaaa
     2161 tcaaagttgg tttattgact tacttgaggc gattaaacaa cctaaaggta aaccaactag
     2221 tcactaacta acgtttacgc gatgcatact tatcgacgta ttcttgccca ccgagttcca
     2281 ttaagttgta catgatttca tcagtaatgg atcgaagaac gaagcggtct tcttccattc
     2341 cgtagtagcg ggaaaagtcc aacggctttc caattcgcaa accaacgtca acattgaaat
     2401 ttggccgact ccgtccaatt ggctgaactt tttcagtatt tatcatcgca actggaataa
     2461 cttttacttt tgcgtccaat gccatccgcg ctacgccagt tttaccgcgg tataaccgac
     2521 catctggcga gcgtgt
//
LOCUS       DBNHVN010000297         5704 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1468329, whole genome shotgun sequence.
ACCESSION   DBNHVN010000297 DBNHVN010000000
VERSION     DBNHVN010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1468329"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..566
                     /locus_tag="AC16Y1_05555"
     CDS             84..566
                     /locus_tag="AC16Y1_05555"
                     /inference="COORDINATES: protein motif:HMM:NF025520.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4307 domain-containing protein"
                     /protein_id="HIR5831959.1"
                     /translation="MQPESQWPAHIKARYGLTKKSKLPFLIGLIVASVVAIGLGMANY
                     RQVNPVIDWYILSFNVQSDKRVDVEWQVARQENRETFCVVRAQNEKRQDVGYATVVIP
                     PGAAKTTATYGLNTESLAVIAEVLGCAYKKEMRVPPADFPPGVKIPAQDPPGFAPTSN
                     "
     gene            584..1066
                     /gene="greA"
                     /locus_tag="AC16Y1_05560"
     CDS             584..1066
                     /gene="greA"
                     /locus_tag="AC16Y1_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015772164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR5831960.1"
                     /translation="MNETWLTQEAFDRLQAELAHRSGEFREEIKNRIAAAREEGDLKE
                     NGGYHAAREEQGKNEGRIRQLAKMLEEAKIGTPSIEDGLVSQGMVVTVHFPDLDDTET
                     FLLGSREEAAHVSIDVYSPTSPLGAAVLGKEIGQEVAYETPTGKSLRIKIISAEIYQG
                     "
     gene            complement(1067..1918)
                     /locus_tag="AC16Y1_05565"
     CDS             complement(1067..1918)
                     /locus_tag="AC16Y1_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5831961.1"
                     /translation="MAIDTKITSTQRPALGWLFTDTMAVAKRHVLKITRVPESLFFSL
                     IQPVMFVLLFAFIFGGAINLGEGGGPQAYREFLIPGIFAQTVMFAVAGITVGITEDAD
                     KGIMDRFRSLPMRPGAVLGGHTLATLIENTLVLVMLSITGLLVGWRIRGSFLDALTAY
                     ALLILFAYAVTWVGAWIGLHMPNPQVAGTAGLAWIFPLTFASSVFTPIATMPSWLQGF
                     ALWNPVSCLALATRELFGNPVPFPGNSFPEQYPVLVSFGYVVLLLAIFAPLSVRAYKT
                     RNSGGKA"
     gene            complement(1921..2910)
                     /locus_tag="AC16Y1_05570"
     CDS             complement(1921..2910)
                     /locus_tag="AC16Y1_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5831962.1"
                     /translation="MSSAIEVTGLAKNFGKVKALNGISFEVEKGTVVGLLGPNGSGKT
                     TTVRILSTLLKPDGGSAKVSGFDVVSQDEDVRRLIGLTGQYAAVDEYLTGRENLELFG
                     RLFHLSKMDAAKRAAELLERFDLSDAADRGIKGYSGGMRRRLDLAASLIGHPSVLFLD
                     EPTTGLDPRSRLGMWEVIKDLVADGTTVLLTTQYLEEADQLASHIIVLDHGNVIAQGT
                     SDQLKTSVGGDRIEVVVDKDDQVAKAAEILKALASGESVVDSMARRILIPVSGGSKAI
                     VEAVRRLDEANILVDDISLRRPTLDDVFLSLTGHIAEEEKEEVPAGRGRRGGK"
     gene            3016..4233
                     /gene="ilvA"
                     /locus_tag="AC16Y1_05575"
     CDS             3016..4233
                     /gene="ilvA"
                     /locus_tag="AC16Y1_05575"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020118470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR5831963.1"
                     /translation="MKVNPVTLDDIYSAQELLKPIIRQTPLIPAGWSKKMYGLDLSFK
                     PENLQRAGSFKIRGAYNRISRLSDSERAKGVVAASAGNHAQGVALAAQLLGIRATIFM
                     PNGAPIPKFEATRGYGAEVHFHGTTIDEALVAAQDFSKENGAIFIHPFDHEHIVAGQG
                     TVGLEMLEQNPEIKTAVVCLGGGGLLAGSAFAIKSLRPDVKVIGVQAKEAAAYPPSLA
                     AGSPQKLARMATMADGIAVGRPGDIPFELIKNYVDEVRVVSEESLSRALLLTLERSKL
                     MAEPAGIAAVAAIMDDPDSFEGPVVATISGGNIDPLLLSKVVRSGLAYAGRFYTLQVR
                     IPDVPGALAKLLMAVASTGSNVIHVSHTRIDPTLAVSEVDVDLELETRGFDHRDEVLR
                     KLTADGFQIIDSF"
     gene            4234..5451
                     /locus_tag="AC16Y1_05580"
     CDS             4234..5451
                     /locus_tag="AC16Y1_05580"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5831964.1"
                     /translation="MRNPGKSLAQRYFQDFPREVSVLTAVGFFVALGYGMVVPIIPVF
                     AKSFGVSTFAATSVVSVFAAMRLIAATPAGRFVNRFGERLMLWTGLSFVAISTFLAGL
                     SQTFIQLLILRGMGGLGSVMFSVASMSLLMRSVEKEKRGRASSTYQGGFLLGSLSGPA
                     LGALFVNENIRLPFFFYTVTLVMAIATAYIALPKGLGRTHVDDPETQEPAMPAREAMR
                     QRRYWAALVANFANGFSSFGLRVALIPLFVIEALQGEPRLASFGFFVAAIVQAAFLFP
                     AGQISDFKSRKVAMLIGNALLTVGLVSIIWQENVSTYFIAMALMGGATAFLSSGPTAT
                     IGDIVGERRGGPVVASYQMTNDFGAIIGPLILGALHDSTGGYAIPFTVALVIVLISFA
                     TTFTMPANKKLTN"
     gene            complement(5440..>5704)
                     /locus_tag="AC16Y1_05585"
     CDS             complement(5440..>5704)
                     /locus_tag="AC16Y1_05585"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020017024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HIR5831965.1"
                     /translation="GNDIGTQYRSAIYWTNDNQEKVARETARLYNEVLVSKGYGAITT
                     ELASSEGKPFWYAEEYHQQYLIKNPNGYDCHAHTGIDLPELVS"
BASE COUNT         1555 a         1294 c         1281 g         1574 t
ORIGIN      
        1 ctatcttaag ccgatttagt gtgcggatct gcacaggttc caatcatcac aaaatcgcat
       61 agtgacttat ccttgaatta gttatgcaac ctgaatcgca atggccagcg cacataaaag
      121 cgcgttacgg gctaacaaaa aaatcaaaac taccattttt aattggtttg attgttgcca
      181 gcgttgtcgc cattggatta ggcatggcta actatcgaca ggttaatcca gttattgact
      241 ggtacatact ttcgttcaac gtccaaagcg ataaacgtgt cgatgttgaa tggcaagttg
      301 ctcgacaaga aaatcgtgaa actttttgtg ttgttcgggc acaaaatgaa aaacgtcaag
      361 atgttggtta tgcaacagtt gtaattccac ctggagctgc aaaaacgaca gcgacttatg
      421 gattaaatac cgaaagtttg gcagttattg ctgaagtact cggctgtgct tataagaaag
      481 aaatgcgggt tcccccggct gattttccgc caggcgtgaa aattccggct caagatccac
      541 ctgggttcgc gccaactagc aattaacttg taatcttttt caaatgaacg agacttggtt
      601 aacacaagag gctttcgatc gccttcaggc agagctagcc catcgaagtg gcgagttccg
      661 cgaagaaatt aagaatcgga tcgccgctgc gcgcgaagaa ggcgacttaa aagaaaacgg
      721 tggttaccac gcagctcgtg aagagcaggg taaaaatgaa ggacgaattc gccaattagc
      781 caagatgctc gaagaagcaa aaattggtac accgtcaatt gaagatggtt tggtttcgca
      841 aggcatggta gttactgtgc acttccccga tttagatgac accgaaacct ttttacttgg
      901 ctctcgtgaa gaggctgcac atgtctcgat cgatgtgtat tcaccgacta gtccattggg
      961 cgcagcagta ctcggcaaag aaattggtca agaagttgct tatgaaactc caactggaaa
     1021 atcactacgg ataaaaatta ttagcgcaga gatttaccaa ggctaattag gcttttccgc
     1081 ctgaatttct agtcttgtaa gcacgtaccg acaacggtgc gaaaattgcg agtaacaaaa
     1141 ctacataccc aaacgaaact aaaactgggt attgttctgg aaatgaatta cctggaaacg
     1201 gaactgggtt accaaataat tcacgagttg ctaacgctaa acaagaaact ggattccaca
     1261 acgcaaaacc ttgtaaccaa cttggcattg tggcaatcgg tgtgaaaaca cttgaggcaa
     1321 atgtaagcgg gaaaatccaa gctaagcctg cggttccagc aacttgtggg tttggcatat
     1381 gtaaaccaat ccaggcacct acccaagtaa cggcataagc aaacagaatc agcagagcat
     1441 aggcagtgag cgcatccaaa aatgatccgc gaattcgcca accaacaagt aagcctgtga
     1501 ttgaaagcat tactaagacc aaagtgtttt caatcagcgt ggcaagagta tgaccaccaa
     1561 gtaccgctcc aggtcgcatc ggtaaggatc taaaccgatc cataattcct ttgtcggcgt
     1621 cttcagtaat accaactgtg attccggcga ctgcaaacat cacagtctgg gcaaaaatac
     1681 ctggaattaa gaattcacga tatgcctgtg gtccgccacc ctcgcctaag ttaatcgcac
     1741 caccaaagat gaatgcgaaa agtaaaacga acatgactgg ctgaatcaac gaaaagaaaa
     1801 gtgactcagg tacgcgagtg atctttaaga cgtgacgttt ggcaactgcc atggtgtcag
     1861 taaataacca acctaatgct gggcgttgcg tacttgtaat ttttgtgtca attgccataa
     1921 ctacttacca cctctgcggc cgcggccagc tgggacttct tctttttctt cttcggcaat
     1981 gtgtccagtt aaggaaagga acacatcgtc aagtgttggt cgccgtagcg aaatgtcgtc
     2041 aaccaaaata ttggcttcat ctaagcggcg cacggcttca acaattgctt tagaaccgcc
     2101 agaaactgga attaaaattc gtcgagccat ggagtcaacc acggattcac cagatgccaa
     2161 tgccttcaaa atttcagcgg ccttcgcaac ttgatcatcc ttgtcaacga caacttcgat
     2221 gcgatcgcca ccaactgaag tttttaattg gtctgaagta ccttgggcaa tcacattgcc
     2281 gtgatctaaa acgataatgt gcgaagctaa ttgatctgct tcttctaggt attgggtggt
     2341 caatagcact gtggtgccat ctgccactaa atctttaatt acttcccaca tgccaagtcg
     2401 gctacgcgga tccaaaccag tagttggttc atccaagaaa agtactgatg gatgtccaat
     2461 caggcttgca gctaaatcta agcgtcgacg cataccgccg gagtaacctt taattcctcg
     2521 atcagcggcg tctgataaat caaaacgttc taataattca gcagcacgtt tagctgcatc
     2581 cattttggaa agatgaaata accgaccaaa aagctctaag ttttctcggc ccgttaagta
     2641 ctcatcaact gcggcatact gaccggttaa tccgataagc cgacgaacat cttcatcttg
     2701 gctgaccaca tcaaagccac tgacttttgc gctaccgcca tcaggcttta acagagtaga
     2761 caagattcgc acggtagtcg ttttacctga accgttgggt ccaagcaggc caactactgt
     2821 gcctttctcc acttcaaaac tgattccatt tagggcttta actttgccaa aatttttagc
     2881 taaacctgtt acttcaattg ccgaactcat tcactgcctt acctaggatt tgagccttct
     2941 taacctaaca cttccgaggg ttaacagtgt ttatcgacct tgctatttgg agttagttag
     3001 taaccatgcc acgatatgaa ggtgaatccg gtaacgcttg atgacatcta ctctgcccaa
     3061 gagttactca agccaataat tcgtcaaact ccacttattc cagctggctg gagtaaaaaa
     3121 atgtacggat tggatttaag ttttaagcca gaaaatttac aacgggctgg ctcattcaaa
     3181 attcgtggtg cttataaccg catctcacga ttaagcgata gtgagcgtgc caagggtgtc
     3241 gttgcggcaa gtgcgggtaa tcatgcgcaa ggtgttgcct tagcggctca actacttggc
     3301 attcgcgcga caatctttat gccaaatggt gcaccgattc caaaatttga ggcaacccgt
     3361 ggctacggtg ccgaggtaca ttttcatggc accacaattg atgaagcgct agtcgctgct
     3421 caagattttt caaaagaaaa cggtgcaatt ttcattcatc cgtttgatca cgaacacata
     3481 gttgctggcc agggaactgt cggcttagaa atgctcgagc aaaatccaga aattaaaacc
     3541 gctgtggttt gtttaggcgg cggtggttta cttgctggaa gtgcttttgc gattaagtcg
     3601 ctgcgcccag atgtaaaagt tattggcgtg caagcaaaag aagctgctgc ttacccacca
     3661 tcacttgctg ccggttcacc acaaaaactt gctcggatgg caactatggc tgacggtatt
     3721 gccgttggtc gccctggtga cattccattt gaattaatta aaaactacgt tgatgaagtc
     3781 agagttgtct ctgaagagtc actgtctcgc gcgctactac taactcttga acgcagtaaa
     3841 ttaatggctg aacctgctgg tattgcagcc gttgccgcaa tcatggatga tcctgactct
     3901 ttcgaaggac cagttgttgc aactatttct ggcggcaaca ttgacccact tttactgagc
     3961 aaagtagtgc gaagtggttt agcttatgct ggccggttct acacacttca agttcgtatt
     4021 cctgatgttc caggggccct tgcaaaatta ctcatggctg tggcaagtac cggttcaaat
     4081 gttattcacg tatcccacac gcgtattgac ccgactcttg cggttagtga agtagatgtg
     4141 gatttggagt tagaaacccg cggctttgac catcgtgatg aagtcttacg taaattaact
     4201 gctgatggat ttcaaatcat cgatagtttc taaatgcgaa atcctggtaa atcactagcc
     4261 caacgttatt ttcaagactt tcctcgagaa gtttcggtct taacggctgt cggatttttc
     4321 gtagcacttg gctatggaat ggtcgtgcca attattccgg tattcgctaa atcattcggg
     4381 gtcagcacat ttgctgcaac atcagtagtt agcgtctttg ctgccatgcg attaattgcc
     4441 gcgactccag ctggcaggtt tgtaaaccgt tttggtgaac gcttaatgct ttggactggc
     4501 cttagttttg tggcaatctc aacatttcta gctgggcttt cgcaaacatt tattcagtta
     4561 ttaattctgc gcggcatggg cggcttaggt tcagttatgt tttcggttgc ctcgatgtca
     4621 cttttaatgc gaagtgtcga gaaagaaaag cggggtcgag ccagtagcac ttatcagggt
     4681 ggctttttat tgggcagctt aagcggccct gcactcggcg ctttatttgt taatgaaaat
     4741 atccgactgc cctttttctt ttacacagtt actttggtta tggcgatcgc aactgcttac
     4801 atcgccttac ccaaaggttt aggtcgaact catgttgatg atccagaaac tcaagaacct
     4861 gcaatgccag cgcgcgaagc aatgcgccaa cgacgctatt gggctgcatt agttgcaaat
     4921 ttcgccaatg gtttttcatc atttggctta cgcgttgcac ttattccact ttttgtaatc
     4981 gaagcactgc aaggcgaacc acgattagct agtttcggtt tctttgtagc ggcaatcgtc
     5041 caggctgcat ttttatttcc agccggacaa atcagcgact ttaagagtcg caaagtggca
     5101 atgctgattg gtaatgccct gttaactgtg ggcttagttt caatcatttg gcaagaaaac
     5161 gtttcgactt acttcattgc aatggcgctg atgggtggtg ctactgcatt cttaagttca
     5221 ggccccactg caactattgg tgacattgtg ggtgaacgtc gcggcggacc agttgtggct
     5281 agttaccaaa tgacaaatga ttttggtgca attatcggac cactaattct gggtgcacta
     5341 catgattcaa caggtggata cgcaattcca tttaccgtgg ctttagtaat tgttttaatt
     5401 tcatttgcca ccacattcac tatgccagca aataaaaaac taactaacta gttcaggtaa
     5461 atcgatcccc gtgtgggcgt ggcagtcata accgtttgga ttcttgatca aatactgttg
     5521 atgatattcc tcggcgtacc agaaaggttt accttcgcta ctggccaact ctgttgtgat
     5581 tgcgccgtaa cccttactaa ctaaaacttc gttatatagc cgggctgtct cgcgggcaac
     5641 tttttcttgg ttatcgtttg tccaataaat tgcacttcgg tattgggtgc caatgtcgtt
     5701 acct
//
LOCUS       DBNHVN010000298        13924 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_649032, whole genome shotgun sequence.
ACCESSION   DBNHVN010000298 DBNHVN010000000
VERSION     DBNHVN010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 13924)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13924)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13924
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_649032"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..686
                     /locus_tag="AC16Y1_05590"
     CDS             147..686
                     /locus_tag="AC16Y1_05590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HIR5831966.1"
                     /translation="MNWLTPKAQARPAGDKGWGSFAIEKIKAGETIAGFGGWCVTREM
                     LNEISEDRQHRSIQVDENLYLVSKDEREAGDCLNHSCDPNCGIMGSTILVAMRDIEIG
                     DELCFDYATCDDSDYDEFICGCGTKACRGTVTGLDWKRPELRIKYNGWFSAYLARKIL
                     AEETAALAELTANEKVSLA"
     gene            complement(678..1724)
                     /locus_tag="AC16Y1_05595"
     CDS             complement(678..1724)
                     /locus_tag="AC16Y1_05595"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR5831967.1"
                     /translation="MAEPRAARRAYEFVRASVSIPENKKSPWYGLSLRFLLAIGLLVL
                     NAVIVYVDRDGYRDANGDGLSWIDCLYYSSVTLSTTGYGDISPVTESARLTNFLLLTP
                     LRFAFIALFVGTTIEVLTNRSKQAFREARWRKTVDQQYVVIGYGVKGRSAVKRLLANN
                     VPPKAIVVVESTHDFDFDLQESGVVAVYGDARREETLRTAQVEEAKQIIVAVDNDDSA
                     VLITLVARRLNSRANIVVAVRESVHNKTFKQSGANTVITTDESTGNLLADTALSPLAG
                     DVLSDLLDPTEGLEVYQREVTKSDIGKSPTQLADIGDLVVSVIRDGVEYRFAYGEIEQ
                     LQAGDKIVSVRNLG"
     gene            complement(1727..2455)
                     /locus_tag="AC16Y1_05600"
     CDS             complement(1727..2455)
                     /locus_tag="AC16Y1_05600"
                     /inference="COORDINATES: protein motif:HMM:NF039977.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6069 family protein"
                     /protein_id="HIR5831968.1"
                     /translation="MAKLARQVTPPAWRIWSYGLAATTFAALANYGWLYLATEIFNWN
                     LLVPKTFSTTELTALTDVRVIIATAIAGLVGTFGATVLAKSVIGPKIWWLIIGFGFGL
                     ASLYGALTLPNVDLSVRLGLSVMHIIATFSIVIPVGWALEIRDHDLKKAVDRYSTHLE
                     ANNPSATTSEVVHPTAVISAEDISKSVIGQEVNDAIAKIEAVGLTARITRRDAEVFTT
                     TFDFREDRINLEVENGTVVSTNVG"
     gene            complement(2622..2706)
                     /locus_tag="AC16Y1_05605"
     tRNA            complement(2622..2706)
                     /locus_tag="AC16Y1_05605"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2670..2672),aa:Ser,seq:tga)
     gene            complement(2819..3679)
                     /locus_tag="AC16Y1_05610"
     CDS             complement(2819..3679)
                     /locus_tag="AC16Y1_05610"
                     /EC_number="3.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF039614.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016403 - dimethylargininase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylarginine dimethylaminohydrolase family
                     protein"
                     /protein_id="HIR5831969.1"
                     /translation="MKNFGVSSSVANLKRVAVRTPTKDADYAAAHWLGAPQELDLDAL
                     LRDHQIFVQLLKDLGCEVEILPEALGLPDAIFVYDPAFVIPSGVVQLQGAKEARVKEP
                     ALLAGDLNALGIPTVGVLTGAATADGGDLFWLDSKTIGLGRTYRTNAEGEKQLRAIFA
                     QDGIEVISFHMPHAMGPEFCLHMMSVISPVREDLAVVYEKEAPVTLLQELESRNIDTV
                     LVPDEEYLSLACNVLPVRPGVVVMPDGNPVTEKRLREKGVEVHTYPASVINRGEGGPT
                     CLTRPIWRED"
     gene            complement(3721..5484)
                     /locus_tag="AC16Y1_05615"
     CDS             complement(3721..5484)
                     /locus_tag="AC16Y1_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831970.1"
                     /translation="MKNSKAHEFFVSFAGPKSTSPLVALPILVFLSFGALFTETLVNG
                     ASKIQLLEMSAIGFAVSFIYFLTFGKLINSFTQVGSITRAAWLAILFFTTEMIRTLLL
                     GWEVLQKNLAASVQWDYRIVAGGLTGVLFFGVRSVVLNDSIRYKENRIQLAEAQERLR
                     KLTSVTQDDLDKNKNRIVNSIQEAVNQALRSVVFDRSTDKKNTKFVVDELVRVSDEVV
                     RPLSRELMNDKFDFVENLEKKQKLTFLGVKIFRLATIVQPFHPTITALFGLLQFIGLA
                     IFLTKNPVVGVLTVLGFTTWIFTILFLAKKYIQPKLAGINIFFRIVVINLIFWLMSSF
                     LLLDSRLSSNLMLPPAGEIYIYLLIIGTIIPWLFAVYSAIKAARSEILENIQETNEKL
                     NWSNARLGAKLWAEQKKLAAMVHRDIQGALISRALKFKQDIESGVDSNVAIGPIREFI
                     EGISTAIYAEIELADPKDEIEKLNSLWDGIFKISAEVSDETRSRIKHDPICWQIVNDF
                     IGEFATNSVKHGKASLGEIKIEQAEEKIIKMIMVNNGLPLPAQMNHGLGSQMVLQQSL
                     NVVHENLPNGGVYFAATIPIN"
     gene            5707..5795
                     /locus_tag="AC16Y1_05620"
     tRNA            5707..5795
                     /locus_tag="AC16Y1_05620"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5743..5745,aa:Ser,seq:gct)
     gene            complement(5967..7199)
                     /locus_tag="AC16Y1_05625"
     CDS             complement(5967..7199)
                     /locus_tag="AC16Y1_05625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5831971.1"
                     /translation="MRTLETEILVVGAGPAGSSAAAWAARNGHEVTIIDKEKFPRDKP
                     CGDGLTPRAVKELTSLGLADWLATKPKNIGLRAAGFGQELLLPWKSPSLPDWGSAVPR
                     LELDNQILEVARAAGVSINDGCAAEDVEVVNGAINKVITKTANETIEYKVKNVIIADG
                     VRSHLGRKLNRTWHRDTAYGVAARAYIKSDRHDDPWISSHLELRGSENEILSGYGWIF
                     PQGNGEVNIGVGTLATQKRPADINLRALISQYTEEQRENWKLEGDLRLPWSAMLPMGG
                     AVSGVAGHNYMFIGDAAGCVNPLNGEGIDYGLETGRLAAELINTENDFSEIWPTLLKD
                     QYGYAFSIARRLAGLLTVPGLLPALGPIGMRSRSLMKVALRVMGNLVTPEDEDLVARI
                     WRKSGALSVKADHRPPFS"
     gene            complement(7256..7894)
                     /gene="upp"
                     /locus_tag="AC16Y1_05630"
     CDS             complement(7256..7894)
                     /gene="upp"
                     /locus_tag="AC16Y1_05630"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010149128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR5831972.1"
                     /translation="MRILITEHPLISHKITALRDERTPSPTFRQLAEELVTLLAYEAT
                     RDIRVEPVTIQTPVAKTVGMQLSQPTPVVIPILRAGLGMLEGMTRLLPTSEVGFLGML
                     RNEETLEASTYADRIPGNLAGRQVFLLDPMLATGGTLISAIELMLQRGANKIVAITLL
                     SAPEGIAAVENAFKDQEVDITIVTGALDEKLNEKGYIVPGLGDAGDRLFGVV"
     gene            8126..9181
                     /locus_tag="AC16Y1_05635"
     CDS             8126..9181
                     /locus_tag="AC16Y1_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013001240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIR5831973.1"
                     /translation="MKKGLKIASVMAAAALVLSACGGGSDEAAATAEPTSAASTVKVG
                     LAYDVGGRGDGSFNDAAYAGLSRAESELGVEIKEVEAGADETDASREERLRLLASEGY
                     NPVIAVGFAYSAAVGAVAAEFPETNFAIVDSVVEGANVASLVFAAEQGSYLVGAIAAT
                     ASKSGVIGFIGGMEIPLIKAFEAGYIQGAKSVNPDIEVLSEYMGPAGDNTAWNVPDKA
                     KTATAAMIDKGVDIAYAAAGGSGLGMFQAIQAAGGPAEGLWAIGVDSDQYTVPSLADV
                     KDVILTSMLKRVDTAVFEVIKGVVDGAPLTGVQNFDLARDGVGYATSNAAVNEFKAAA
                     DAAAEAIKSGSVTVAVE"
     gene            9309..10835
                     /locus_tag="AC16Y1_05640"
     CDS             9309..10835
                     /locus_tag="AC16Y1_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5831974.1"
                     /translation="MANSENAVELTGIWKRFPGVIANSNVNLKVKRGSAHAIVGENGA
                     GKSTLMKILYGMQKPDEGQILVNDVAQDFDSPSDAIEVGIGMVHQHFMLADNLTVLEN
                     IILGSEIKKGIRLDLATATEKISEIAKQYRLELPLNEYVSELGVGDRQRIEILKVLYR
                     GATILILDEPTAVLVPHEVDELFKALNDLKSEGITIIFISHKLDEVLEIADEITVIRN
                     GSTVGTVSPKTQQVTAEDLAEMMVGSELPTPATTESTVTDKVVLEVNDLTVLTEGGKA
                     LVDHASFKIHAGEILGIAGVEGNGQAELLDALLGIEKSTGTVKYEGSDISNWPTRKRR
                     ENGIAYIPEDRSRQALLLEAPLWENRMLGHQTLAPSSKGMWLTPKTAKADTARIVAEY
                     DVRTPSIDVFASALSGGNQQKLIVGREMSGEPKLLIAAHPTRGVDVGAQAAIWDHLRA
                     ARAAGLAVLLISADLDELIGLSDSLKVILRGKFVADADPATVTPKQLGNAMTGAGAAA
                     "
     gene            10835..11929
                     /locus_tag="AC16Y1_05645"
     CDS             10835..11929
                     /locus_tag="AC16Y1_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5831975.1"
                     /translation="MKIRKFIEVLAAPILAIVFSLIVTTIILAAFGANIVEVFSRMFD
                     YGTSTRSLTLIVNTATTYYLSAVAVAIGFKMNLFNIGVNGQYRLAAMTAGAIAGWVVL
                     PGPLTVIAAILAAMVVGALWAGIAAWLKIVRGISEVISTIMLNFIAIGVISYIIVQTN
                     VGSEVRLNVRGTPVVPEGARLVGFNLIDPKVKVYGFFFIAIAVGIIYWVVLNRTRFGY
                     DLRASGLSPSAAKASGVNSKRMVLYAMLASGSVAGLVGLPDLLGKEFSYTLTFPANYG
                     FTGIAIALLGRNNPIGIAFGALLWAFLDVSSQILDLEGVPREIVTIMQGVIVLSVVVA
                     YELVRRRKVITQQRNVGKALAAASPSEGGK"
     gene            11929..13152
                     /locus_tag="AC16Y1_05650"
     CDS             11929..13152
                     /locus_tag="AC16Y1_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5831976.1"
                     /translation="MAALTLPKTKLVRFALLLLAALFALSLARTFADTADLTSTGTIA
                     AAVGLAVPIGLAGLGGIWSERSGVANIGLEGMMILGTFGAGWAGWQFGPWVGMASAVI
                     YGAVGAFIFAIATVSFGVDQIIAGTAINILGFGSTQFLAKLAFANAPGGGQTQSPPVS
                     NLPSFTVESIAGPLSDFQKQGIFLLSDAAGLLSGFFYQVSILTLLAISLFVLTSFIMW
                     RTSFGLRLRSSGENPWAAETLGVNVYKYKYIAVTVSGALAGLAGAYLAMVGTNVYREG
                     QTAGRGYIGLATMIFGNWRPGGTAIGAVIFGYIDALQLRDKSNINGLTLLGAVVLIAI
                     AIMMLIRGMRGTGMALGFLGAALLSAFLLTTKMPTEVALVAPYVTTLLVLATASQSLR
                     PPAADGVPYRRGESH"
     gene            13153..13548
                     /locus_tag="AC16Y1_05655"
     CDS             13153..13548
                     /locus_tag="AC16Y1_05655"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013772009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIR5831977.1"
                     /translation="MEINWESLREHATTAMKNAYAPYSDFPVGVAALVNDGRIITGCN
                     VENASYGVGLCAECGLVSNLINSGGGRLIAVACVDRHGNALMPCGRCRQLLWEHGGAE
                     CLLDSVSGIKPMREILPDAFGPDDLINRK"
     gene            13596..>13924
                     /locus_tag="AC16Y1_05660"
     CDS             13596..>13924
                     /locus_tag="AC16Y1_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HIR5831978.1"
                     /translation="MNNTKPEPFSAPEVIATKRDRGELSDAQIDWFVDAYVRNVIAEE
                     QMSAMAMAILLNGMNRREIVRWTQSYINSGETMDWSKLGRPTADKHSTGGVGDKITLP
                     LAPLVAA"
BASE COUNT         3873 a         2998 c         3058 g         3995 t
ORIGIN      
        1 catctctggc acttttggcg atacctggac caggttttct cttggccaaa aattataaat
       61 atttggctaa gtggcactaa catcgaatct gcacttattt gttactacgc tcgctcgagc
      121 agaagcatga ggccacctaa ttggacatga actggttgac tccgaaggcg caggctcgcc
      181 cagctggaga taaaggttgg ggcagtttcg ctatagaaaa aattaaagcc ggagaaacca
      241 ttgctggttt tggcggatgg tgcgtaactc gcgaaatgct aaatgaaatt tctgaagatc
      301 gccagcatag atccatacag gttgatgaaa atttatatct tgtttctaaa gatgaacgag
      361 aagccggtga ctgtttaaac cactcatgtg atccaaattg cggaattatg ggcagcacta
      421 ttttggttgc catgcgcgac attgaaattg gcgacgaact ttgctttgat tacgcaactt
      481 gtgacgattc agattatgac gaattcatct gcggatgtgg aacaaaagct tgtcgcggta
      541 ccgtaactgg tcttgactgg aaacgtccag aattgagaat taaatacaat ggttggttct
      601 cggcgtacct agccagaaaa attttggccg aagaaactgc agccttagcc gaattaactg
      661 cgaacgaaaa agtttcgcta gcctaagttt ctgaccgaaa ctattttgtc accggcttgt
      721 aattgttcta tctcgccgta cgcaaaacga tattcgacgc catctctaat tacgctcaca
      781 accaaatcgc cgatgtcggc taattgagtt gggctcttac cgatatcact ctttgtgact
      841 tcacgttgat atacctctaa accttcagtt ggatctagta ggtcacttaa tacatcacct
      901 gctagcggtg atagcgctgt atctgcaagt aaatttccgg ttgattcgtc agtagtaata
      961 actgtgtttg caccagattg tttaaacgtc ttattgtgca cactttcacg aaccgcaaca
     1021 acaatgtttg ctcgtgaatt taatcttctt gcaaccaagg tgattaacac ggccgaatca
     1081 tcattatcga ccgcgacaat tatttgcttt gcttcttcaa cttgagctgt gcgcaaagtt
     1141 tcttcacgtc gagcatcacc ataaaccgct actacacctg attcttgaag atcgaaatca
     1201 aaatcatgtg tgctctcaac tacaacgatt gctttaggtg gcacattgtt tgccagtagg
     1261 cgttttacag cagaccgacc tttaactcca tacccaataa ctacatactg ctgatccact
     1321 gttttcctcc accgagcttc acgaaatgct tgcttactcc gatttgtcag tacttcaatt
     1381 gttgtaccta caaaaagtgc tatgaatgca aaacgcaacg gagttagtaa taaaaaattt
     1441 gtaagtcttg cagattctgt aactggagaa atgtcgccat aaccagtggt tgaaagtgtg
     1501 acgctcgaat agtaaagaca gtcaatccaa cttaatccat caccattagc atctcgataa
     1561 ccatcgcgat cgacgtaaac aattactgca ttaagtacaa gtaacccgat agctaataaa
     1621 aatcttaacg atagtccata ccaagggctt tttttgtttt cgggaatcga gaccgaagca
     1681 cgaacaaatt catatgctct tcgtgccgct cttggttcag ccatatttaa ccaacgtttg
     1741 tcgaaactac tgttccgttt tcaacttcta aattgattct atcttcgcga aaatcaaaag
     1801 ttgtggtaaa aacttctgca tcgcgccgtg taattcgagc cgttaaacct actgcttcga
     1861 tttttgcgat tgcatcatta acttcttggc caatgactga ctttgaaata tcttcggcac
     1921 taataacagc agttggatga acaacttcac ttgttgtggc tgaaggatta tttgcttcta
     1981 aatgagtgct gtaacgatcc acagccttct tcaaatcatg atcgcgtatt tctaacgccc
     2041 agccaactgg aatcacaata gaaaacgtag cgattatgtg cataacgctt aggccaaggc
     2101 gcacggatag atcgacattt ggaagagtta gtgcgccata aagtgaagct aagccaaaac
     2161 caaaaccaat aattaaccac caaatttttg gaccgattac tgatttggct aatactgttg
     2221 caccaaaagt tccaactagg cctgctattg ccgttgcaat aatgacccga acgtcagtaa
     2281 gtgcggttaa ttcagtcgtg gaaaaagttt taggaactaa taaattccaa ttgaaaattt
     2341 cggtagctaa atacagccaa ccataattcg ccaatgctgc gaatgtggtt gcggctaatc
     2401 cgtaactcca gattcgccat gcgggtggag taacttgacg agctaacttc gccatatcgt
     2461 gcctttctga ttcacccgaa actttgctca attccctttg tggaaatttt tggcggcgag
     2521 ggagggaacc gtcgctcgcg ctattcgcac gagctcctcc caaatccctc gttgcttacc
     2581 aagcgtaaac aacgattccc gaaagggaat cttcgtttac gcggcgaggg agggatttga
     2641 acccccggag gcttacacct cagacgcttt caaggcgccc gcattagtcc gctctgccac
     2701 ctcgcccggc tatataaata gccacacgca agattacgcg attagcaata cataagggaa
     2761 atctcggcgc aaaatccttc tagtcttcgc gccattactt actaggcgca aaatccttct
     2821 agtcttcgcg ccatatcggc ctggtcaaac acgttggtcc accctcacca cgattaatca
     2881 cgctggctgg ataagtgtga acttctacac ccttttcgcg caaacgtttt tcagttactg
     2941 ggtttccatc aggcataaca actacgcctg gacgaactgg taatacattg cacgctaacg
     3001 acaaatactc ttcatctggt accaagacgg tatctatatt gcgagattcc aattcttgaa
     3061 gcagcgtaac cggagcttcc ttttcataaa ccacagctaa atcctcacgg accggtgaaa
     3121 taacactcat catgtgtagg caaaattctg gacccattgc atgtggcatg tggaagctaa
     3181 ttacttcaat tccatcttgc gcaaaaattg cacgtagttg cttttcgcct tcggcatttg
     3241 tgcgataggt acggcctaat ccaatagttt tactatccaa ccaaaaaagg tcgccaccgt
     3301 ctgcagttgc agctccagta agtaccccga cagttggaat gcctaaggca tttaaatctc
     3361 cagctaaaag tgccggttct ttaacccgag cctctttggc cccttgtaat tgaactacgc
     3421 cactaggaat aacaaatgct ggatcgtaaa caaatatggc atctggcaaa cctagcgctt
     3481 cgggcaggat ttctacctcg cacccaagat cttttaataa ctgaacaaat atttgatgat
     3541 ctcgaagaag tgcatctaga tccagttctt gaggtgcacc taaccagtgt gcggcagcgt
     3601 aatctgcatc tttagttggg gttctaactg caactcgttt taggtttgct actgatgaac
     3661 taactccgaa atttttcata gacacatact tctagagaaa attaaaaata acaagtacga
     3721 ctaattaatt ggaatagttg ctgcaaagta aactccacca tttggcaagt tttcgtgtac
     3781 aacgttaagt gattgctgta aaaccatttg cgatccaagt ccatgattca tctgtgctgg
     3841 aagtggtaat ccgttgttta ccattatcat ttttattatt ttttcttccg cttgctcaat
     3901 ctttatttca ccaagactag ctttaccgtg tttaacagaa ttggttgcaa attctccaat
     3961 gaagtcattt acaatttgcc agcaaatcgg atcatgctta attctggagc gggtctcgtc
     4021 actgacttca gctgaaattt tgaaaatgcc atcccaaaga ctatttaatt tttcaatttc
     4081 atcctttgga tcggccaact cgatttcagc ataaatagca gtggagatac cttcgataaa
     4141 ttcacggatg ggtccgattg ccacatttga atccacaccg gattcaatat cttgtttaaa
     4201 cttaagtgct ctggagatta aagcgccctg gatatcgcga tgcaccatcg cagcaagttt
     4261 cttttgctca gcccaaagtt tggcgcctaa tcgcgcatta gaccaattaa gtttctcatt
     4321 tgtttcttgt atgttttcca aaatctcgct acgtgcggct ttaatagctg aataaacagc
     4381 aaacagccaa ggaataatcg ttccgattat caataggtaa atataaattt cacctgctgg
     4441 tggcaacatt aaattacttg aaagtcttga atccagtaaa agaaatgaag acattaacca
     4501 aaaaattaaa ttaataacga caattcgaaa gaaaatattt attccagcaa gtttaggttg
     4561 gatgtatttc tttgctaaaa agagaatcgt aaaaatccat gtagtaaagc caagcacagt
     4621 tagaactcca accactggat tcttggttag aaatatcgca agcccgatga actgaagtaa
     4681 gccaaatagc gcggtgatag ttgggtgaaa tggttgaaca attgtggcta gtctaaatat
     4741 tttgacgcct aaaaaagtca acttctgttt tttttctaaa ttttcaacaa aatcaaattt
     4801 gtcgttcatt agttcacggc taagtggcct aaccacttcg tcagataccc gcactaattc
     4861 atcgacaaca aactttgtat tttttttatc agttgatcgg tcaaatacaa cacttcgcag
     4921 ggcttgatta actgcttctt gtattgaatt aacaattcga ttcttatttt tatccaaatc
     4981 atcttgagtt accgaggtta atttgcgtag tctttcttga gcctcagcta actgaattcg
     5041 attttcttta taacgaattg agtcatttaa aacaacagag cgcacgccaa aaaataaaac
     5101 accagtaagg cctccagcaa ctattcggta atcccattga actgaagcgg ctaaattttt
     5161 ctgaagtacc tcccagccca ataaaagtgt ccggatcatt tcagtggtga agaaaagtat
     5221 tgccaaccag gcggcccgag taatcgaacc aacttgagtg aagctattaa ttaattttcc
     5281 gaaagttaaa aaataaataa aactaactgc aaatccaatt gctgacattt caagtaactg
     5341 aattttagat gcgccgttaa ctaaagtttc agtgaaaagt gccccaaaag ataggaacac
     5401 caaaatgggt agcgcaacaa gcggtgaagt agattttggt ccagcaaaac taacgaagaa
     5461 ctcgtgggcc ttactatttt tcaatacgtt caattggttt accgaacttt aattagagta
     5521 agttgttcgc aaaaccgaga atcatgcctt actcagttaa ttcttctggg cgctgggctt
     5581 cttcgcaatt tgaaatataa gttcacaatg tgcatccaga aaagaagccc gcattattgg
     5641 gctaaaatcc aataattgag agtttttcgc catatctacg gataaccgat aatctcttga
     5701 cgattaggag atgtcgcata gcccggtcga gtgcgcctca ctgctaatga ggtaaggggt
     5761 aaaacccttc ggaggttcaa atcctctcat ctccgcaaat gagtaagact ccgcttgtgc
     5821 ggggtcttac tcatttgatc ggaaaataaa tgaagaggat ttgggaggcc gatgtttagc
     5881 cgaaggcttt aaacatcgga acaataaaga ctgtatagcg acggtccctc tcatctttaa
     5941 tcggaacaat aaatactgca tacccactaa ctaaacggtg ggcgatgatc tgctttaact
     6001 gaaagtgcgc ctgacttacg ccatatgcga gcaactaaat cttcatcttc tggggtaact
     6061 aagttaccca ttacgcgcag cgcaactttc attaatgagc gagaacgcat gcctattggt
     6121 cctagtgctg gcagtaaccc gggaacagtc aataagccag ccagtctgcg ggcaattgaa
     6181 aaggcgtaac catattgatc tttaagtaat gtcggccaaa tttcggaaaa gtcattctca
     6241 gtatttataa gctctgcagc tagtcgacca gtttccagtc cgtaatcaat gccttcacca
     6301 ttaagtggat ttacacaacc agcagcgtca ccaataaaca tgtaattatg tccagcaacg
     6361 cccgaaactg ctccacccat tggcaacatg gctgaccatg gaaggcgtaa atcaccttcg
     6421 agtttccaat tttcgcgttg ttcctcggtg tattgagaaa ttaatgcgcg caaattaatg
     6481 tctgctggtc gtttttgcgt tgctagggtt ccaacaccaa tatttacttc gccattacct
     6541 tgcggaaaaa tccaaccata cccagaaaga atttcattct cggaaccacg aagttctaaa
     6601 tgagaagaaa tccaagggtc atcgtggcga tcgcttttta tatacgctct ggcagctact
     6661 ccatatgcgg tatctcgatg ccaggttcga ttcaattttc gacccaaatg tgagcgcaca
     6721 ccatcagcaa taattacgtt tttaactttg tactcaatag tttcattagc cgtttttgta
     6781 atgaccttat taatagctcc atttacaact tcgacatctt cggctgcaca tccatcatta
     6841 atactgacac cggctgccct tgctacttca agaatttgat tatcaagttc aaggcgtgga
     6901 accgcacttc cccaatcggg taaggatgga cttttccacg gtagtaagag ttcttgacca
     6961 aagcctgctg ctcgaagccc gatatttttt ggtttagtag caagccaatc ggccaacccc
     7021 aatgaagtta attcttttac tgcgcgcgga gttaaaccat cgccacaagg tttatcccgc
     7081 ggaaatttct ctttatcaat aatggttact tcgtgaccat tgcgcgctgc ccaagctgct
     7141 gctgagctgc cggcaggacc ggctccaacc actaaaattt cagtttctaa tgtgcgcact
     7201 tgttcattct ctcgcagtta aattatttgc gcatatttaa aaatgatggc ttgatttaaa
     7261 caacgccaaa taagcgatcg ccagcatcgc caagacctgg cactatgtag cctttttcat
     7321 ttaatttttc gtctaatgcg ccagtgacaa tggtgatatc gacttcttga tctttaaatg
     7381 cattttcaac tgcagcaata ccctcgggtg cactaagtaa tgtaatggca acaattttgt
     7441 tcgcgccacg ttgcaacata agttcaattg cagagattag cgttccacca gttgccaaca
     7501 ttggatctaa taagaaaact tgacgcccag ctaaattacc tgggatgcga tctgcataag
     7561 tagaagcttc taaagtttct tcgttgcgca acatgccaag aaaaccaact tcagaagttg
     7621 gcagaagtcg cgtcatacct tcaagcatgc ccaagcctgc gcgcaaaata ggaattacaa
     7681 ctggtgttgg ttgcgaaagt tgcattccga cagtctttgc caccggagtt tgaatagtta
     7741 ctggctcaac ccgaatatct cgagtcgctt cataagcaag aagtgtgact aactcttcgg
     7801 caagttgacg aaaagttggc gaaggagttc gttcatcgcg caatgccgta attttgtgtg
     7861 aaattaatgg atgctcagta atcaagatgc gcacggtaca agagtacgcg cctgactatt
     7921 taatagagca gcatgtccca atccaagttg tgcgaacaga atgttactgg tgtgacgtgt
     7981 gtgattaatt ctcgcttgcc aaagttacaa cttggtgacg attggccaac actcgaccgc
     8041 caagtaactc gtaaaacacc tttgatttaa actgatttat agacaatgtg atactcacaa
     8101 agttgcttaa gagaggagcc catctgtgaa gaagggttta aaaattgctt cagttatggc
     8161 tgcagcagcg cttgtactaa gtgcttgcgg tggcggtagc gatgaagccg ctgcaactgc
     8221 cgaaccgaca agtgcagcaa gcaccgttaa ggtcggtctt gcatatgatg tcggcggtcg
     8281 tggcgacggt tcattcaacg atgcggcata cgctggttta tctcgtgctg aatctgaact
     8341 tggcgtagaa atcaaggaag ttgaagctgg tgcagacgag actgacgcaa gtcgtgaaga
     8401 acgtctacgt cttctagctt cagaaggtta caacccagtt attgcagttg gctttgctta
     8461 ctctgctgct gttggtgccg tagctgctga attcccagaa actaactttg cgatcgttga
     8521 ctctgtagtc gaaggtgcaa acgttgctag cctggtattc gctgctgaac aaggttcata
     8581 cctagttggc gcaattgctg ctactgcaag taagtcagga gtcattggct ttattggtgg
     8641 tatggaaatt ccactaatca aggctttcga agctggttac atccaaggtg caaaatcagt
     8701 taacccagat atcgaagttc tatctgaata catgggtcca gctggtgaca acactgcatg
     8761 gaacgttcca gacaaggcaa agactgcaac tgcagcaatg atcgacaagg gagttgacat
     8821 tgcttacgca gctgctggtg gttctggtct aggtatgttc caggcaatcc aagctgctgg
     8881 tggacctgct gaaggtctat gggcaattgg tgttgactca gaccagtaca cagttccgtc
     8941 gctagctgat gttaaggatg taatccttac ttcgatgcta aagcgcgttg acactgctgt
     9001 attcgaagtg atcaagggcg tagtagatgg cgcaccacta actggtgtcc agaactttga
     9061 ccttgctcgc gacggtgttg gctatgccac atcaaacgct gccgtaaatg aattcaaggc
     9121 tgcagccgat gctgccgctg aagcaatcaa gagcggttcc gtaacagtcg ctgttgagta
     9181 atcaatagtt aactaaaggt cgttggcccg ggtggaaata tctacccggg ccacactttt
     9241 aaccccagat agtcgtaagt ttgggttaat aaaacttaat aagtccctac gagaagtggc
     9301 taggaattat ggctaattca gaaaatgccg ttgagttaac tggaatctgg aaacgtttcc
     9361 cgggcgtaat tgcaaatagc aacgttaact taaaagttaa acgtggcagc gcgcatgcaa
     9421 tcgtgggtga aaacggtgct ggtaaatcta ctttaatgaa aattctgtat ggcatgcaaa
     9481 aaccagacga aggacaaatt ttagttaacg atgtagcgca agatttcgat tctccatctg
     9541 atgcaatcga agtcggtata ggcatggtgc atcagcactt tatgttggcc gacaatctca
     9601 ctgttttgga aaacataatt ctgggcagcg aaattaaaaa aggaatccgg cttgatctgg
     9661 caacagcaac tgaaaaaatt tcagagattg caaaacaata ccgcctcgaa ttgccgctaa
     9721 atgaatatgt ctcagagctt ggcgttggcg atcgtcaacg cattgaaatc ttaaaagttt
     9781 tataccgcgg cgcaactatt ttaattttag acgaaccaac tgcggtttta gttccgcatg
     9841 aagtcgatga attattcaaa gccctaaatg acttaaagtc tgaaggcatc acaattattt
     9901 ttattagcca caaactagat gaggttttag agattgcgga tgagataacc gtgattcgaa
     9961 atgggtctac tgtcggaaca gttagtccaa aaactcaaca agttaccgcc gaagacttgg
    10021 ctgaaatgat ggttggcagt gaattaccta ctcctgccac tactgaaagt acagttaccg
    10081 acaaagttgt tttagaagtt aatgacttaa cagtgctcac tgaaggtggc aaggctttag
    10141 tagatcatgc aagtttcaaa atacatgctg gtgaaatatt aggtattgct ggtgttgaag
    10201 gaaacggtca agctgaatta cttgatgcgt tacttggaat cgaaaaatca actggcacgg
    10261 tgaaatatga gggaagtgat atttcaaatt ggccaactcg caaacgacgt gagaatggta
    10321 ttgcctacat tccagaagat cgttcgcgcc aagcattact acttgaagca ccactttggg
    10381 aaaatcgcat gctagggcat caaactctcg ccccaagttc aaaaggtatg tggcttaccc
    10441 ctaagacagc taaagccgac actgctcgga ttgttgcaga atacgatgtg cgcacaccaa
    10501 gcattgatgt atttgcaagt gcactttcgg gtggtaacca acaaaaatta attgtgggcc
    10561 gagaaatgtc tggtgaacca aaactactta ttgcggctca tccaactcgt ggtgtggatg
    10621 tcggcgctca agcagcaatt tgggatcatt tacgtgcggc tcgggcagct ggtttagctg
    10681 tattactaat cagtgcggac cttgatgaat tgattgggtt aagcgattca ctgaaagtga
    10741 ttcttcgcgg aaaatttgtg gcagatgcag atccagcaac tgtgacaccg aaacaacttg
    10801 gtaatgcgat gaccggtgcg ggagcggctg cgtaatgaaa attcgtaaat ttattgaagt
    10861 tttagccgcg ccaattttag cgattgtttt ctcgctgata gttaccacaa ttattttggc
    10921 ggcctttggc gcaaacatag tcgaagtatt tagtcgaatg tttgattacg gtacttctac
    10981 tcgttcacta actttaattg taaatactgc cactacttac tacttaagtg cggtagcggt
    11041 tgcaattggt ttcaagatga acttgttcaa cattggtgtg aacggacaat atcggttagc
    11101 tgcaatgact gcaggtgcaa tcgcaggctg ggttgtactg ccaggtccac taactgtgat
    11161 tgctgcaatt ttggctgcaa tggttgtcgg tgcactatgg gctggaattg ccgcttggtt
    11221 aaaaattgtg cgcggtatca gtgaagttat ttcaactatc atgcttaact tcattgcaat
    11281 cggagttatt agttacatta ttgtgcaaac aaatgtcggt agcgaagtaa gacttaatgt
    11341 gcgaggcact ccggttgttc cagagggtgc tcgattagtt ggttttaact taattgatcc
    11401 aaaagtcaag gtttatggat tcttcttcat tgcaattgcc gtaggaatta tctactgggt
    11461 tgtgctcaat cgcactcgtt ttggatatga cttaagagca agtggactgt caccttctgc
    11521 agcaaaggca agtggagtta acagcaagcg catggtgctt tatgcgatgt tagcttctgg
    11581 ttctgttgcc ggacttgttg gtttaccaga tttacttggt aaagaatttt cttacacact
    11641 tactttcccg gctaactacg gtttcaccgg aattgcaatt gctttgcttg ggcgcaataa
    11701 cccgataggt atcgcatttg gtgcattact ttgggcattc ttagatgtat cgagtcagat
    11761 tcttgatcta gaaggcgtac cgagagaaat tgtcacaatt atgcaaggtg tgattgtgct
    11821 ttctgttgtt gtagcttacg aattagttag acgccgcaag gttattactc aacaacgaaa
    11881 tgtgggtaaa gctttggctg cggcgagccc aagtgaagga ggtaagtaat ggctgcactt
    11941 actcttccta aaacaaaatt agttcgcttt gctctgttat tgcttgcggc gttatttgca
    12001 ctttcgctgg cgcgtacttt tgcagacact gcagacctaa ctagtactgg aacaattgct
    12061 gctgctgttg gtttagctgt accaattggc ctagcgggct tgggtggcat ctggtctgaa
    12121 cgttctggtg ttgccaacat tggccttgaa ggcatgatga ttcttggaac ttttggtgca
    12181 ggttgggctg gttggcaatt tggaccatgg gtcgggatgg cttcagcagt tatctacggt
    12241 gctgttggcg catttatttt tgcaatagca acagtttctt ttggtgtaga tcaaattatt
    12301 gctggtacgg caattaatat tctgggattt ggttcaactc aattcctggc gaaattagct
    12361 tttgcaaatg ctcctggagg tggtcaaact cagtccccac cagtttcaaa cttgccaagt
    12421 tttaccgtcg aatcaattgc tggaccactt tctgattttc agaagcaagg tattttctta
    12481 ctaagtgatg ctgctgggtt actgtctgga ttcttttatc aagtttcaat acttactttg
    12541 ctggcgatct cactctttgt gctgacttct ttcattatgt ggcgcacttc atttggtctt
    12601 cgattacgaa gttccggtga aaatccgtgg gcagctgaga cgcttggcgt aaatgtttac
    12661 aagtacaaat acattgcggt aactgtttca ggtgcacttg ctggacttgc tggtgcatat
    12721 ctagccatgg ttggcaccaa cgtttaccgc gaaggacaaa cggctggacg cggttacatc
    12781 ggtttagcca cgatgatttt tggtaactgg cgaccaggtg gaacggctat tggcgcagta
    12841 atttttggtt acatcgatgc actacagctt cgagataaat caaatatcaa tggattaact
    12901 ttgcttggcg ctgttgtgct aattgcaatt gcgatcatga tgttgattcg cggcatgcgc
    12961 ggtactggta tggcacttgg atttcttggc gctgcgctac ttagcgcatt tttgctcacc
    13021 accaagatgc caactgaagt tgcattagtt gcgccatacg taactacctt gttggtgcta
    13081 gcaacggcat cacaaagttt gcgcccgcca gctgccgatg gtgtacctta ccgcagaggc
    13141 gaatcacatt aaatggaaat taactgggaa agtttgcgcg aacatgccac aacggcaatg
    13201 aaaaatgcat atgcacctta ttcagatttt cccgtgggtg tagctgcact agtaaatgac
    13261 ggtcgcatta ttactggatg taacgttgaa aatgcttctt acggagttgg actttgcgca
    13321 gaatgtggct tagtttcaaa cttaattaat tctggtggtg gtcgcttaat tgcggttgcc
    13381 tgtgtcgatc gccatggaaa tgcacttatg ccttgtggtc gttgccggca gctactttgg
    13441 gagcatggcg gcgctgagtg tttacttgat tcagtttccg ggataaaacc aatgcgcgaa
    13501 atattgcctg atgcatttgg tcctgacgat ttaataaacc gtaaataaaa attaaccatt
    13561 aactaaagcc cgatttgcat ggcaagatgg gtttaatgaa taacaccaag ccagagccgt
    13621 tttctgctcc cgaggttatt gcaaccaagc gtgaccgggg tgaattatcc gatgcacaaa
    13681 tcgattggtt tgtggatgct tatgtgcgaa atgtaatcgc tgaagaacaa atgtcggcga
    13741 tggcgatggc gattttgcta aatggaatga atcgtcgcga gatcgttcgt tggacccaga
    13801 gttatataaa ttctggcgaa actatggatt ggtctaagtt aggacgccca actgcagata
    13861 aacattcaac tggtggcgtt ggtgacaaaa ttactttgcc acttgctccc ttggttgctg
    13921 cctg
//
LOCUS       DBNHVN010000299         5850 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1691767, whole genome shotgun sequence.
ACCESSION   DBNHVN010000299 DBNHVN010000000
VERSION     DBNHVN010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1691767"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="AC16Y1_05665"
     CDS             complement(<1..255)
                     /locus_tag="AC16Y1_05665"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR5831979.1"
                     /translation="MQFDGLVVPAITQFDETGQVDAKGVAEFTEVLISQGVHGIVACG
                     TTGEAYSLTPDERHLVISTVMKQVAGRIPVLSGVGGMSTNT"
     gene            392..973
                     /gene="trmB"
                     /locus_tag="AC16Y1_05670"
     CDS             392..973
                     /gene="trmB"
                     /locus_tag="AC16Y1_05670"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF014447.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(46)-N(7))-methyltransferase TrmB"
                     /protein_id="HIR5831980.1"
                     /translation="MAFEQYVPIYQIPLPDRLINLQEVFGHKDIYVDFGCGMGGHTID
                     LLEQGKNVLAIDVHTAGICDLSNYAHDNNLETFRLFHGDGIDLFEKILPKTIKQIDVY
                     FPDPWPKARHAKRRLFSESFLQLANNLLVTEGRLVLVTDDDSYAQHVVDVLGTQDYFS
                     EVEFKQSVTMTSFHARALKLGHNIHKFELVNKT"
     gene            complement(977..1513)
                     /locus_tag="AC16Y1_05675"
     CDS             complement(977..1513)
                     /locus_tag="AC16Y1_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase family protein"
                     /protein_id="HIR5831981.1"
                     /translation="MTDITDSISTWLPSDELENARAKLPIVYVDAIPVRIDEAGRVLK
                     IGLLLRGRPDGSISRAVISGRVLFGERIRDALLRHIEKDLGSMALPQIPTNPAPFTVA
                     EYFPDPNVSGFHDPRQHAVSLVYVVPVKGDCQPSQSALDLVWITPHEAVSRQVTAEMT
                     GGQDLLVRLALAHTGALP"
     gene            complement(1524..2375)
                     /locus_tag="AC16Y1_05680"
     CDS             complement(1524..2375)
                     /locus_tag="AC16Y1_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015882806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5831982.1"
                     /translation="MTTATNDLGSSRHRVVDLQDVTVHRGGKNILAHINWQVSANERW
                     VIMGPNGAGKTTLMQICTGFLHPTTGSAQILGEKLGLTDVRELRTRIGLSSAALNNYL
                     PLDETVFNTVLTAAYAVTGRWRERYDEVDFARTHKLLGDWGLTGFADRLIGTLSEGER
                     KRTHIARALMSDPEILILDEPAAGLDVAGREDLVSRLAQLAVDPDSPCLILVTHHVEE
                     IPPNFTHALLLSDGQVSSLGEVQEVIQSEPMSVAFKTPLDVEFGDDRWYARGRTPSRG
                     RRARSNM"
     gene            2394..3092
                     /locus_tag="AC16Y1_05685"
     CDS             2394..3092
                     /locus_tag="AC16Y1_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5831983.1"
                     /translation="MGFPAAIELAIWLNQVRNGSISTTDAVNAAETITQSLVIEPDAD
                     LGSTSASDWPALVELAKSFKVPCFALLPSPGHPYGLSAHVISRVDPNFGVCILGDEYL
                     LAKTNSQDPTWFLTDLPNTIPALDPKSARLVLQDLIEKSSVELSSMELTGNREFVEQE
                     LHQQRPIHLPPALSNRVRNDLELAERIWLIAQISMNEAQAPHSRSLDEQKLNRLRELR
                     HAAINLMTASTLAA"
     gene            complement(3124..3897)
                     /gene="fabI"
                     /locus_tag="AC16Y1_05690"
     CDS             complement(3124..3897)
                     /gene="fabI"
                     /locus_tag="AC16Y1_05690"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIR5831984.1"
                     /translation="MGILDGKNILITGVLTDSSIAFHVARLAQEQGANVVLSSFGRTT
                     SITKRIAGRLPVEPPIVQLDVTNKDDLANLANAIKEHVPHLDGVLHSIAYAPEAALGG
                     NFLNTEWEDVKVALEISAFSLKSLSMACLPLMEKNGGQVLGLDFDATVTWPFYDWVGV
                     SKAALESTCRYLARDLGPKKVRVNLVAAGPLYTTAAKGIPGFDQFGPMWQRRAPIGWD
                     TSDPVPTAKACVALFSDWFPATTGEIIHVDGGVHSQGGP"
     gene            complement(3900..4604)
                     /gene="fabG"
                     /locus_tag="AC16Y1_05695"
     CDS             complement(3900..4604)
                     /gene="fabG"
                     /locus_tag="AC16Y1_05695"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012786512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HIR5831985.1"
                     /translation="MTRVVLVTGGNRGIGLTIAQKFKANGDTVVVTSRSGEAIDGLNV
                     IKCDVADSGSVEAAFDELEQKFGPAQVVVANAGITRDGLVIRMSDNDIDEVLNTNLFG
                     AIRVARRASKAMMKARTGRIIFISSVVGLMGSAGQVNYAASKAGLIGAARSLARELGS
                     RGITTNVIAPGFVDTDMTAQLSEDRRNEIVANVPLGRYASTNEIADVVTFIASEQAGY
                     ITGAVIPVDGGLGMGH"
     gene            4623..>5850
                     /locus_tag="AC16Y1_05700"
     CDS             4623..>5850
                     /locus_tag="AC16Y1_05700"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018545868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR5831986.1"
                     /translation="MNIISVTPLAGGQISPERLAEIALSVVAKAGVTVGQLRIAQHDT
                     RGVGLRDGKVENVATDTSIALSVRVVKDGAWGFAATTDLTEFGAKDTANRAVEMAKLS
                     AAVSTDRVELVDEPSYGKVSWRLPIAIDPMLVSDEQVIELLKSWHEKVAAHSIVHHVD
                     VHAAMGKDITFFADLTGNLITQERDRTSAQLTAVHVSESGFDDMRTCAPPAGRGFEYL
                     QGQGWNWDDELTNLPEYLSERTKAPSVTAGKWDLVIDPTNLWLTIHESIGHATELDRA
                     LGYEANYAGSSFATIDKLNNFKYGSEIMNITGDRNAPHGLSTVGYDDEGVAAQQWDLI
                     KDGTLVGYQTDRNIAKKIGLTRSNGCAFADSALHSPIQRMPNVSLQPGVEDLSNADLI
                     ARVDRGIYIVGDKSWSI"
BASE COUNT         1591 a         1385 c         1312 g         1562 t
ORIGIN      
        1 agtattcgtt gacataccac cgacgcctga gagcaccgga attcgaccag caacctgttt
       61 cataactgtg ctgattacca aatgtcgctc atccggagtc agcgaatatg cttcaccagt
      121 tgttccacaa gctacgattc catggacacc ttggctgatt agtacctcag taaattcagc
      181 aacgcctttt gcgtcaactt ggccagtttc atcaaattga gtgattgctg ggacgactaa
      241 accgtcgaat tgcactttat ccccctgtgt tcggctcttg tagccttgcc atatgctgcc
      301 tcaaatctac ccgaacgaat ctggtctgtt gggtcgacct cggattcgaa gtttccaggg
      361 acggcgtggt cgaatatcac agagtcgaag cttggcattc gaacagtatg tcccgattta
      421 ccaaattcct ctgcccgatc ggttaatcaa tctacaagag gtttttggtc acaaagatat
      481 atacgtcgat tttggctgtg gcatgggtgg tcacacaatt gatttactcg agcaaggtaa
      541 aaacgtctta gcaatcgatg tacataccgc ggggatctgt gacttaagta actatgcaca
      601 tgacaacaat ctagaaacat ttagattgtt tcatggtgat ggaatcgatt tgttcgaaaa
      661 aatactacct aaaactatta agcagatcga tgtatatttc cctgatccgt ggccaaaagc
      721 acggcatgcc aaacggcgtt tgtttagtga gtcatttttg cagctggcta ataatcttct
      781 tgtaactgaa ggtcgacttg tccttgttac tgatgatgac tcttatgcgc agcacgtagt
      841 tgatgtgctt ggaactcaag attatttttc tgaagttgaa tttaagcaaa gtgttacgat
      901 gacttcattt cacgccaggg cattaaaact tggtcataat attcacaagt tcgagttagt
      961 taacaaaact tgaagattaa ggcaaggctc cggtgtgagc caaggccaat cgcacgagaa
     1021 gatcttgacc ccctgtcatt tcagcagtta cctgtcggga aactgcctcg tgtggcgtta
     1081 tccaaactaa atccaatgcc gattgcgatg gttggcagtc acctttaact ggaactacat
     1141 aaactaacga cactgcatgt tgtcttggat catgaaatcc gctgacattt ggatcaggaa
     1201 aatactctgc aaccgtaaat ggagctgggt tagtcggaat ttgtggcagt gccatggatc
     1261 ctaaatcttt ttcaatgtga cgtaatagcg catcccggat ccgttcacca aaaagaaccc
     1321 gaccggagat gactgcacga cttatcgatc catctgggcg gccacgcaaa agtaaaccaa
     1381 tttttaaaac gcgaccagct tcatcgatcc ggactggaat tgcatcaacg taaacgatcg
     1441 gtagcttggc gcgggcattt tctaattcat cgcttggtaa ccaagtagag atggaatcgg
     1501 ttatgtcagt cacagtctta ttgttacatg ttggagcgag cgcggcgtcc tcttgacggt
     1561 gttcgacccc tggcgtacca ccgatcgtca ccaaattcga catcaagcgg tgttttaaag
     1621 gcgacactca tcggttcgct ttgaataact tcctgcactt cgcccaaaga actgacctgg
     1681 ccatctgaca gcaatagagc gtgggtgaaa ttcggcggta tctcttcgac gtggtgcgta
     1741 accaaaatca aacatggact atctggatca acagctagtt gagccaaccg actaactaaa
     1801 tcttcgcgac cggctacatc caggccagcg gctggttcat ccaaaatcaa aatttctgga
     1861 tcactcatta aagctcgagc aatatgggtt cgcttccgct ccccctcaga taaggtaccg
     1921 attaatcgat cggcaaaacc agttagtccc caatcaccca gcaatttgtg tgtccgggca
     1981 aaatcaactt cgtcatatcg ctcgcgccat cgtccagtga ctgcataggc cgctgtaagt
     2041 actgtattaa aaactgtttc atcaagcggt aagtagttat ttaatgctgc gctcgacaat
     2101 ccaatccgag tgcgaagctc acgcacatcg gttagcccaa gcttttcacc caaaatttgt
     2161 gcacttccag tagtcggatg caaaaatcca gtacatatct gcattagcgt ggtttttcct
     2221 gcgccattcg gacccataat gacccagcgt tcattagcac ttacttgcca attgatatgc
     2281 gccagaatat tttttccgcc tcggtgaact gtgacatctt gtagatctac gacacggtgt
     2341 cgagaactcc ctaagtcatt tgtcgcagta gtcacagtag taatctaccg ttcatgggat
     2401 ttccggccgc gatcgaatta gctatttggc taaaccaagt tcgaaatggc tcgatatcaa
     2461 caacagatgc ggtcaacgct gctgagacca ttacccaatc tctagtcata gaacctgatg
     2521 cagatttagg gtctaccagt gcatcagatt ggccagcact cgttgaatta gccaaaagtt
     2581 ttaaagttcc atgctttgca ctcttaccaa gtcctggaca tccgtatggg ttatctgcgc
     2641 acgtgatttc aagagttgat ccaaatttcg gtgtttgtat tcttggtgat gaatatttgc
     2701 tggctaagac taatagtcag gatccaacct ggtttctaac cgatctgcct aatacgatcc
     2761 cagcgctaga tccaaaatca gcccgattgg ttctacagga tttgatcgag aaaagcagcg
     2821 ttgaattaag ttcgatggag ttgactggaa atcgtgaatt tgtcgaacaa gagttacatc
     2881 aacaaaggcc gatccatttg ccacctgccc tatcaaatcg tgttcgcaac gatcttgaat
     2941 tagctgaacg tatttggtta attgcccaaa ttagtatgaa cgaagcgcaa gcgccacaca
     3001 gtcgaagtct tgatgagcaa aaattaaatc ggcttcgtga acttcgtcat gctgcaatca
     3061 atttgatgac tgcaagtacg ctagccgcct agcggttaaa agtgctcaca cgctgcgcaa
     3121 ggatcacggc ccgccttgag aatgtacgcc accatcgacg tggataatct caccggttgt
     3181 ggccgggaac caatcactaa atagcgcaac gcatgcttta gctgttggca ccggatcact
     3241 tgtatcccaa ccgatcggcg cacgtcgttg ccacatcgga ccaaactgat cgaaacctgg
     3301 aataccttta gctgcagttg tgtaaagcgg accggcagca actaaattaa cccgaacttt
     3361 ctttggtcct aaatctctgg ctagataccg acaagttgat tcgagtgctg cttttgatac
     3421 acccacccag tcgtaaaacg gccaagtcac agttgcgtca aaatctagac caagtacttg
     3481 cccaccattt ttttccatca aaggtaaaca cgccatgctt agcgatttaa gtgagaatgc
     3541 agaaatttct aaagcgactt ttacatcttc ccattcggtg tttagaaagt ttccacctaa
     3601 tgcggcttca ggcgcatacg cgatggaatg taaaacaccg tcaaggtgtg gaacatgttc
     3661 tttaatcgcg ttagccaaat tagccaaatc atctttatta gtaacatcta gttgaacaat
     3721 cggcggctct accggcaggc gaccagcgat ccttttggtg atgcttgttg ttcggccaaa
     3781 cgagctaagc acaacatttg caccctgctc ttgagccaat cttgcaacgt gaaacgcgat
     3841 agatgaatcc gttagtacac cggtgattaa aatattttta ccgtcaagga tgcccataat
     3901 taatgtccca ttcctaatcc accatcaact ggaataacag cgcctgtgat ataaccggct
     3961 tgttcagaag caataaaggt gaccacgtca gcaatctcat ttgtacttgc atatcggcca
     4021 agaggaacgt ttgccacaat ctcgtttcga cgatcttcag acagttgtgc tgtcatatcc
     4081 gtgtcaacga aaccaggagc aataacattt gtggtaattc cacgagaacc aagttcacga
     4141 gcaagtgacc tagcggcgcc gattaaaccg gcctttgagg ctgcgtaatt gacttgtccg
     4201 gccgaaccca ttaatccaac gacacttgaa ataaaaatga tccgaccagt gcgggccttc
     4261 atcatcgcct tacttgcacg tcttgctacc cggatggcgc caaataaatt agtgtttaat
     4321 acttcatcaa tgtcgttatc cgacattcga ataactaggc cgtcgcgcgt gattccggca
     4381 ttagcgacca ccacttgggc tggaccaaac ttttgttcga gttcatcgaa ggcagcctcg
     4441 acactgccac tatccgctac atcacatttg atgacattca aaccgtcgat agcttcgcca
     4501 gatcggctgg ttacgacaac cgtgtcacca ttggctttga atttttgagc aatcgtcaat
     4561 ccgattcctc gattgccacc tgttaccagc acaacacggg tcattggtct aggttatcgg
     4621 taatgaacat tatttcggta acaccattgg ccggcgggca gatatctcct gagcgattag
     4681 cagaaattgc gctttctgtc gttgccaagg ctggtgtaac ggtgggtcaa cttcgcattg
     4741 cacaacatga cacaagaggt gttggtttgc gcgatggcaa agttgagaat gttgcaacgg
     4801 acacttcgat agcgttaagt gttcgagtcg ttaaagatgg cgcatggggt tttgctgcca
     4861 caactgactt aactgaattt ggtgcaaaag acactgctaa tcgagctgtg gaaatggcta
     4921 aattaagtgc cgcagtctct actgatcgcg ttgagttagt tgatgagcca agctatggaa
     4981 aagtttcttg gcgcttacca atagcaatcg atccgatgtt ggtttcagat gaacaggtaa
     5041 ttgagttact caagtcatgg catgagaagg tagccgctca ttcgattgtt catcacgttg
     5101 atgttcatgc ggcaatgggt aaagacataa cttttttcgc cgatctaact ggaaacctaa
     5161 ttacccagga acgtgatcga acaagtgcgc aattaacggc tgttcatgtc agcgaatctg
     5221 gctttgacga tatgcgcacc tgtgccccac ccgcgggtcg tggctttgag tacttacaag
     5281 gtcaaggttg gaattgggat gacgaattaa caaatctgcc ggagtatctt tcggaaagaa
     5341 ctaaggctcc atcggtcact gcaggtaagt gggatctggt aatcgatcct acaaatctat
     5401 ggctgacaat ccatgaatcg atcggacatg caactgagct tgatcgagcc cttggttacg
     5461 aagcaaacta cgctggatca tcatttgcca cgatcgacaa attaaataat tttaaatacg
     5521 gcagtgaaat tatgaatata actggtgacc gaaatgcacc tcatgggctg agcacagttg
     5581 gctatgacga tgaaggtgta gccgctcaac aatgggatct aattaaagac ggaacgctgg
     5641 ttggttacca aacagatcgg aacattgcta aaaagattgg cttaactaga tcaaatggtt
     5701 gtgcatttgc tgacagtgcg ctgcatagcc caatccaaag aatgccaaac gtttcactgc
     5761 aacctggtgt ggaggattta tctaacgccg atttaatcgc ccgagttgac cgtggaatct
     5821 atattgttgg cgacaagtcc tggtctatcg
//
LOCUS       DBNHVN010000300         8540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1320008, whole genome shotgun sequence.
ACCESSION   DBNHVN010000300 DBNHVN010000000
VERSION     DBNHVN010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 8540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8540
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1320008"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..126
                     /locus_tag="AC16Y1_05705"
     CDS             <1..126
                     /locus_tag="AC16Y1_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR5831987.1"
                     /translation="TGVPLPLVSTGGSSLIPTLAALGLLMSFAKYEAAKEQSQNR"
     gene            190..1284
                     /gene="murG"
                     /locus_tag="AC16Y1_05710"
     CDS             190..1284
                     /gene="murG"
                     /locus_tag="AC16Y1_05710"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016468455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR5831988.1"
                     /translation="MQSGTAKATLNIVVAGGGTAGHIEPAMNLADELKRRHPEAKITA
                     LGTEKGLEVTLVPARGYDLQFIRALALPRKPSLKLLALPILLRAAVVETRALLHVLRA
                     DIVIGFGGYVSVPVYLAARGRVPIVVHEANAKAGLANRLGAQFADAIAVTVPNSLPNS
                     VQTGMPLRESIAQFNRVQIRDEAKAYFGITNDLPTVLVFGGSQGAQSLNKVIEQAKRD
                     GLLEQINIIHSVGKKNDFVFAKSQNYIPLNYIEQMDLAYAAADFVIARSGAMTVAEVT
                     AVGLPACFVPLPIGNGEQKVNAQPVVSAGGAIMVENANFSPDFISSQVLPIVTNSTRL
                     SQMASASKSCGRQDATSRLADVVDHVLKVQ"
     gene            1284..2714
                     /gene="murC"
                     /locus_tag="AC16Y1_05715"
     CDS             1284..2714
                     /gene="murC"
                     /locus_tag="AC16Y1_05715"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR5831989.1"
                     /translation="MSKLAGRVHIVGIGGAGMSGIARILLSKGFQVSGSDAKNSRRLQ
                     SLIPLGAHVYVGHDAQKMLLDGKAPDTVVVSTAIAQTNPEVEHALELGIPVLNRATAL
                     AQVVEGSRVISVAGTHGKTTTTSMTTVILQNSNLDPSFVIGSEMNESGSNAHLGSGKD
                     FVVEADESDGTFLVLPTTAAIVTNVEADHLNFWGTYEAVEQAFIDFVVGSGSKHGFAV
                     VCGDDPGAKKVSERARELGARVINYGQGAHNDAVIKFLPPVASAVEVIPGNIGSRFEV
                     NFAGQVFGPIQLRVPGAHNALNATAALLTCFELGCSADQVVTGLEKFTGTHRRFEYRG
                     IAGGVRVFDDYAHHPTEIDAVLKAARTVAGSGRVLAAFQAHHYYRTALFSKEFGIALG
                     LADFVVVLEVFAPGETPIPGASGSTMAGNVPLPSEQVVFEPSWSAVPKHLVDHAKPGD
                     IIMTLGAGDIGMMCPEILSLLEQKNS"
     gene            2714..3400
                     /locus_tag="AC16Y1_05720"
     CDS             2714..3400
                     /locus_tag="AC16Y1_05720"
                     /inference="COORDINATES: protein motif:HMM:NF020067.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR5831990.1"
                     /translation="MAKTIKAAQKKTRKNSKLPWLAWLVAVVVIALWWSLYQSRWFIT
                     ETVTIQGVSRLTVEQVGAVAGAPLNEPLISQDVDGIAERLNALPEIKTALVERGWPKT
                     LLITITERIPIAVAATAGGFNLIDDEGKNAGLVAAPPAGLLVIAANPDSPAMLSAIQV
                     VAALPAEWQVVGLAATSQDNVVANLASGAVVTFGSGENAKRKVEVASALLAQNYLTVN
                     VSAPEAPSSK"
     gene            3643..4875
                     /gene="ftsZ"
                     /locus_tag="AC16Y1_05725"
     CDS             3643..4875
                     /gene="ftsZ"
                     /locus_tag="AC16Y1_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005478214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR5831991.1"
                     /translation="MPAPQNYLAVIKVVGIGGGGVNAINRMIEQGLKGVEFVAINTDA
                     QALLMSDADVKLDIGRDTTRGLGAGADPEVGRKAAEDHREEIEEILRGADMVFVTAGE
                     GGGTGTGGAPIVARISRDLGALTIGVVTRPFSFEGKRRMTQADEGVEALRSEVDTLIV
                     IPNDRLLELADNGISVLDAFRHADSVLLQGVSGITDLITTPGLINLDYADVRSVMKGA
                     GSALMGIGSARGADRAIEAARQAISSPLLEASIEGARGVLISVSGGSDLGLFEINEAA
                     RMVQGAVHPDALIIFGAVIDDTQGDQVKVTVIAAGFDGGAPPSTKTTSPMAKRVMEET
                     PIAAATAVGLAKEGEAPSIPVASNQTQQSAIASSVDDDDDNFQSLFGSSPVAGDVFDE
                     TSSTDGSEEDLDIPDFLR"
     gene            4885..5592
                     /locus_tag="AC16Y1_05730"
     CDS             4885..5592
                     /locus_tag="AC16Y1_05730"
                     /inference="COORDINATES: protein motif:HMM:NF014619.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH/LACC1
                     family protein"
                     /protein_id="HIR5831992.1"
                     /translation="MSEYARVEIGPISVIALSRHGGVSREPYKSLNLGSYVGDNPNDV
                     AQNFAHVKNLSKADQLTWLNANHGTTVHYAIDPGQAPPGDGLVTTKRKHGIVALSADC
                     VPFALVDPVNQVIAVGHAGWKGVLANLMQVLTQSFVSNGANPDSSHAVIGPSICGACY
                     EVPADRVSLFTDVLPSAIFDQTHLTLSSGVTKVLTELGFTTQQIPGCNFEDENLFSFR
                     KANGAATGRGGLVAIIN"
     gene            5625..6320
                     /locus_tag="AC16Y1_05735"
     CDS             5625..6320
                     /locus_tag="AC16Y1_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR5831993.1"
                     /translation="MERRSELAQNLAEVKADLANACIAANRDPAEVTLIVVTKTWPAS
                     DVELLAQLDVTNVGENRDQEAKVKHGEVTAKLTWHAIGQLQTNKAKSVANWADVVHSV
                     DRLELVAALTKAVAHRPSPLGCLIQVNLDPDPRDERGGANPLDVAELAQAISSTPGLE
                     LRGVMGVAPLDADPNPAFAKLREISANLAREYPTAIWISAGMSGDYIEAINHGATHLR
                     IGSSILGHRQVNG"
     gene            6353..6856
                     /locus_tag="AC16Y1_05740"
     CDS             6353..6856
                     /locus_tag="AC16Y1_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HIR5831994.1"
                     /translation="MPNAMRKMAEYLGLVDGDQYNQSYDALEEYVEYEEQYTQAPLRS
                     VNQNAVAEPTSSYATPTYPAPVARPAAPSNDLYRITTLHPRTYNDARRIGDEFRNGTP
                     VIMNLGDMDDADARRIVDFSAGLAFGLHGSIERVAKGVFLLSPANVDIGAAARAQLRE
                     DPFFNQS"
     gene            6871..7155
                     /locus_tag="AC16Y1_05745"
     CDS             6871..7155
                     /locus_tag="AC16Y1_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013838457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR5831995.1"
                     /translation="MSAITSLIASLLDVYILILILRLVFDYITMFARDWRPRGMVLVL
                     AETLYSLTDPPLNALRRVVPPLRLGSISLDLGFLILIFGVSILRNVFLSL"
     gene            7264..8106
                     /locus_tag="AC16Y1_05750"
     CDS             7264..8106
                     /locus_tag="AC16Y1_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015032937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HIR5831996.1"
                     /translation="MAVTPEDVRNKQFTTSRFKEGYDLDEVDDFLDSIEETLSALTKE
                     VADLRATAKGEIPSSIREEIATLTKENNRLKGELDQAVNSVEKLRAENESLVSAGGDD
                     SALEELRAKLTQSQTELAEARNQLANTSRGQEDLSGEIVRLNSALEELTAANANLAKE
                     NEELKQAAPVMEPGEASSASVRILELAQRTADEAVATARIESENLRNTATAEVERLMR
                     ETNANIQLMTREFESHKANMERKVEELRAYEREYRSRLRSYLEGQLRELEGKTLGGDR
                     QVEG"
     gene            complement(8200..>8540)
                     /locus_tag="AC16Y1_05755"
     CDS             complement(8200..>8540)
                     /locus_tag="AC16Y1_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017591206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HIR5831997.1"
                     /translation="APREGWTVASEGGESLALDLALTPELIQQGIARDITRLIQDGRK
                     SSGFDISDRISVTWQATDAQTKAALENYGQNIAAEVLATNWAPGTVSTVSVADEELGF
                     SAMLVKSNAD"
BASE COUNT         2339 a         1840 c         2093 g         2268 t
ORIGIN      
        1 actggtgtgc cattgccgtt ggtaagtacc ggtggttcct cattaatacc aactttggcc
       61 gcattaggtt tacttatgtc gtttgcaaaa tatgaagcgg ccaaagaaca aagccaaaac
      121 cgttaaaacc aaagttatct acatcaatat cagataattg agttattaaa ttatttgaaa
      181 tgagttttgg tgcaatctgg aacagctaaa gcaactctaa atattgttgt tgctggtggc
      241 ggtaccgctg gccatattga accagccatg aatttagctg atgagctaaa gcgcagacac
      301 ccggaagcaa aaattactgc gcttggcacc gaaaaaggtt tagaagtgac gctggttcca
      361 gcgcggggtt acgacttgca atttattcgg gcactggcgt taccgcgcaa acctagtttg
      421 aaactacttg cactacctat tttgttacgg gctgctgttg ttgaaaccag agcgttactt
      481 catgtgctgc gagcagatat tgtcattggc tttggtggct atgtatcggt tccggtttac
      541 ttggccgcta gaggcagagt gccaattgtg gttcacgaag caaatgctaa agccgggtta
      601 gctaatcggt taggcgctca atttgctgac gctatagcgg taacagtgcc aaatagtttg
      661 cctaattcag tgcaaactgg aatgccgctt cgggaatcta ttgcacaatt caatcgagtc
      721 caaattcgag atgaagccaa ggcgtatttt ggcataacta atgacttacc tactgtgctt
      781 gtatttggtg gctcacaggg cgctcaatca ctaaataaag ttatcgagca ggctaagcga
      841 gatggcttac ttgagcagat aaatatcatt cattcggtcg gtaagaaaaa tgattttgtt
      901 tttgctaaat cccaaaacta cattccactt aattacattg aacaaatgga tttagcttat
      961 gcggcagccg attttgtgat cgccaggtct ggtgcaatga cagttgccga agttaccgca
     1021 gttggattac ccgcatgctt tgtgccactt cccattggca acggcgagca aaaagttaat
     1081 gcgcaacccg tggttagtgc gggcggtgca attatggttg agaatgcaaa tttttcacca
     1141 gactttatct caagccaagt gcttccaatt gttactaact caactcgatt aagccagatg
     1201 gcatcggcga gtaaatcctg tggtcggcaa gatgcaacca gtagattggc agatgtcgta
     1261 gaccacgttc ttaaggtgca gtgatgagca aactagctgg ccgagtccac atcgttggaa
     1321 ttggtggagc tggaatgagt ggaattgcgc gaatcttgct cagcaaaggt ttccaagttt
     1381 ccgggagtga tgcaaaaaat tctcgccgat tgcaatccct tattccatta ggtgcacatg
     1441 tttatgtcgg ccacgatgcg caaaaaatgt tacttgatgg aaaagctcca gataccgtcg
     1501 tggtttcaac tgctattgcc caaacaaatc ccgaagttga acacgcccta gaacttggga
     1561 ttccggtttt gaatcgagca actgctttag cgcaagttgt agaaggcagt cgagtaattt
     1621 cagttgcggg aactcatggc aaaactacaa ctacttcgat gaccacggta attttacaaa
     1681 attccaattt agatccgtca ttcgtgatcg gctctgagat gaatgagtca ggtagtaacg
     1741 ctcaccttgg cagtggaaaa gattttgttg ttgaagcaga cgagagtgat gggacattct
     1801 tagtgctacc aactacagct gcgattgtga caaatgtcga agccgaccat cttaattttt
     1861 ggggtactta tgaagcagtc gaacaagcgt ttattgattt tgtagttggc agtggcagca
     1921 aacatggatt tgcggttgtt tgtggcgatg atccaggtgc taaaaaagtc tcagaacggg
     1981 cccgtgaact cggggctcga gttatcaatt atggtcaggg tgcacataat gatgcggtca
     2041 ttaaattttt gccacctgtt gcaagtgctg ttgaagtaat tccaggcaat atcggctctc
     2101 gctttgaagt gaactttgct ggccaagttt ttggtccgat ccaacttcgc gtacctggtg
     2161 ctcacaatgc gctgaatgca acggcggctt tacttacgtg ttttgagtta gggtgctctg
     2221 ctgaccaagt agttactgga ttagaaaaat ttactggcac acaccgtcgc tttgaatatc
     2281 gtggaattgc tggcggtgtt cgagtctttg atgattacgc acatcatcca accgagatcg
     2341 atgccgtttt gaaagcagct agaacagttg ctggttcagg acgagtgttg gctgcttttc
     2401 aagcgcatca ttactaccgg accgcattgt tttcaaaaga atttgggatc gcacttggat
     2461 tagctgattt tgttgtggtg ttagaagttt ttgcgccagg cgaaactccg atcccaggag
     2521 ctagtggcag cacaatggca ggaaatgtcc cgctgccgag tgaacaagtg gtgttcgagc
     2581 cttcttggtc tgcagtacca aaacacttag tagatcacgc aaaacctggt gacatcatta
     2641 tgactttagg tgctggtgac attgggatga tgtgtccgga aatacttagt ttgcttgagc
     2701 agaaaaattc ttaatggcca aaacaattaa agctgcgcaa aagaaaactc gaaaaaattc
     2761 caaattacct tggcttgctt ggttagttgc agtcgtcgta attgcgttgt ggtggtcact
     2821 gtaccaatcg cgttggttta taaccgaaac cgtcactatc caaggagtat cgcgactaac
     2881 tgttgagcaa gtcggggcag tggccggggc tccactaaat gagccgttga taagtcaaga
     2941 tgttgatgga atcgctgaac gcttaaacgc actacctgaa attaaaactg cgttagttga
     3001 gcgaggttgg ccaaaaacat tattgatcac gataactgaa cgtattccga tcgctgtggc
     3061 ggcaacggct ggcgggttca acttaattga tgacgagggc aagaatgctg gtttagttgc
     3121 agcgccacct gcaggattac tggtgattgc cgccaatccc gacagcccag cgatgttatc
     3181 tgcaattcaa gttgttgctg cgctacctgc agaatggcaa gttgttggtc ttgctgccac
     3241 atcacaagac aatgtcgtgg ctaacttagc cagtggagca gtagttactt ttggctcggg
     3301 tgaaaatgca aagcgcaaag ttgaagttgc atctgcacta ttagcgcaaa actatttaac
     3361 ggtaaatgta agtgcgccag aggcgccaag cagtaaatag ctgaaagcta tttactgcca
     3421 ataagtcctg ctcaatataa ccagaagttg tttgcaggta atagaaactg tgcaagagaa
     3481 actgtgcaat aaaaactgta tgaaataaaa aacaaattta cggcgtggtg taacgaaaaa
     3541 aactagtaaa cgcaataact ttatgtggaa ataggttgac ataactataa ggctctggtt
     3601 gaggtttaga gttattcact tcggggatat gaaaggcctg gcatgccagc accacagaat
     3661 tacttagcag ttattaaagt cgtcggaatc ggcggcggtg gcgtcaacgc aatcaatcgc
     3721 atgattgaac aaggtcttaa aggtgttgag ttcgtcgcga tcaatactga cgctcaagca
     3781 ctgttaatga gcgatgcaga tgtgaaatta gatattggcc gcgacacaac tcgtggctta
     3841 ggcgctggcg ctgatccaga agttggtcgt aaagctgctg aagatcatcg cgaggaaatt
     3901 gaagaaattt tgcgcggtgc agacatggtt tttgtcaccg caggtgaagg tggcggaact
     3961 ggtactggtg gcgcgccaat agttgctcga atctctcgcg acttaggagc cctaactatc
     4021 ggtgttgtaa ctcgtccatt tagttttgaa ggcaagcgtc gtatgactca agccgacgaa
     4081 ggtgttgaag ctctgcgcag tgaagtagat acattaattg tgattccaaa cgatcgctta
     4141 cttgaattgg ctgataacgg aattagtgtt cttgatgctt tccgccatgc tgactcagtt
     4201 ttactgcaag gggttagcgg cattactgac ttaatcacca cacctggctt aattaacttg
     4261 gactatgcag atgtgcgttc ggtcatgaaa ggcgccggtt cagctttgat gggcattggc
     4321 tcggctcgcg gcgctgatcg tgcaatcgaa gcagctcgtc aggcaatctc cagcccacta
     4381 cttgaagcaa gtatcgaagg tgctcgcggc gtcttgatat cagtatccgg tggcagtgac
     4441 ttagggctat ttgaaatcaa cgaagccgcc cggatggttc aaggcgcagt acatccagat
     4501 gctttgatta tttttggtgc agttatcgat gacacccaag gcgatcaagt aaaagtaaca
     4561 gtaattgcgg ctggatttga tggtggtgcg ccgccttcaa ctaagaccac atcaccaatg
     4621 gccaagcgtg taatggaaga aactccaatt gccgcagcca cggcagtcgg cttggctaaa
     4681 gaaggtgaag cgccaagtat tccggtggca agtaatcaaa ctcagcagag tgcaattgct
     4741 agctcagtgg atgatgacga cgataatttc cagagccttt ttggaagttc tccagttgct
     4801 ggcgacgtat ttgatgaaac ttcgagtact gatggctctg aagaagactt agatattcca
     4861 gactttcttc gttagtcgct aagtgtgagc gagtacgccc gagtcgaaat aggtccgatc
     4921 agtgtaattg cactgagtcg ccatggtggg gtaagtcgcg aaccttacaa gtcacttaat
     4981 ttaggtagtt atgtcggcga caatccaaac gatgttgccc aaaattttgc gcacgttaaa
     5041 aacttatcca aggcggacca actaacttgg ctgaatgcta atcatggaac cactgtgcat
     5101 tacgcgattg atccagggca ggcgccacca ggtgatggat tagttactac taaacgcaaa
     5161 catggcatcg tagctttatc ggctgattgt gtgccgtttg cattagttga cccagttaac
     5221 caagtcatcg cagtaggaca tgctgggtgg aagggtgtcc tggcaaattt aatgcaggtg
     5281 ctgacccaaa gttttgtttc taatggcgca aatcctgata gttcacatgc cgtaattggc
     5341 ccatcgattt gtggcgcttg ttatgaagtt ccggcagatc gagtttcttt atttaccgac
     5401 gtcctaccaa gtgcgatttt tgatcaaacg catctaactc tttccagtgg cgtaactaag
     5461 gttttgactg aacttggttt tactacccag caaattcctg gctgtaattt tgaagacgag
     5521 aatttattca gtttccgcaa agcaaatggc gcagccaccg gtcgcggtgg tttagttgcg
     5581 ataattaact aatcaaaaag atttaattta ggtcgcaggt ataaatggaa cgtcgaagtg
     5641 aattagcgca aaatcttgcc gaagtgaaag cagatttagc taatgcctgt attgcggcca
     5701 atcgtgaccc agccgaagtc acgttaattg ttgtaactaa aacttggccc gctagcgatg
     5761 ttgaattatt agcccaactt gatgtgacta atgtgggcga gaatcgagat caagaggcaa
     5821 aagttaaaca cggggaagtt acggccaaat taacttggca cgcaatcggg cagttacaaa
     5881 ctaataaagc aaagtcagta gcaaattggg ctgacgtggt gcacagtgtt gaccggcttg
     5941 aattagttgc agctttgact aaagcagttg cgcacagacc gtcaccactt gggtgtttaa
     6001 ttcaggtcaa cttagatcca gatccacgtg atgaacgtgg gggagcaaat ccgttggacg
     6061 ttgccgaact tgctcaagct atttcctcaa caccgggtct agagctacgc ggagtaatgg
     6121 gggtagctcc gctagatgca gatccaaatc cggcatttgc aaagttaaga gaaatttcgg
     6181 caaatcttgc gcgcgaatat ccaactgcaa tatggatttc agcaggaatg agcggagact
     6241 atatcgaagc gataaatcat ggcgcgacac accttcgaat tggctcgtct atcctgggac
     6301 atcggcaagt taacgggtaa gttttaagaa ggctttcccc aaggagtaac taatgccgaa
     6361 cgcaatgcgc aaaatggccg aatatctagg cctagttgac ggcgatcaat ataaccaaag
     6421 ttatgacgca cttgaagagt acgttgaata cgaagagcaa tacactcaag ctccacttcg
     6481 ctcggtaaat caaaatgcag tagctgagcc gacaagttca tatgcaactc cgacctaccc
     6541 agccccggtt gctcgtccag ccgcgccaag taatgatttg tatcgcatca caaccttgca
     6601 cccacgcacc tacaacgacg cacgtcgtat tggtgacgag tttcgtaacg ggactccagt
     6661 catcatgaac ttaggtgaca tggacgatgc agacgctcga cggattgtcg acttttctgc
     6721 tggtttagca tttggcctac atggcagtat tgaacgagta gccaaaggcg tattcctttt
     6781 gtctccggca aatgttgata tcggcgcagc cgctcgggct cagttgcgtg aagatccatt
     6841 cttcaaccaa agttaattaa gtagtcgatt atgtcggcaa tcacatcact gatcgcatca
     6901 ttacttgatg tttacattct gatattaatt ttgcgcttag tttttgacta cataacaatg
     6961 tttgctcgtg actggcgacc acgcggaatg gttttggttc ttgctgaaac tttgtattct
     7021 ttaactgatc caccacttaa tgcactccga agagttgtcc cgccattgcg acttggttcg
     7081 atttcgttgg atctaggttt cctgatcttg attttcggcg tttccatctt gcgcaacgta
     7141 tttctaagcc tttaacgacc cactggaaag gcgcgagttt ccaaattagc ccatttccgc
     7201 ctaatctagt aaggcgaaga ggggctatcc ccaccagaaa acctaatcga aagggtcttc
     7261 aaaatggcag ttacgccgga agatgtgcgc aacaaacagt tcaccacttc acgttttaaa
     7321 gaaggttacg acttagatga agtagatgat tttctagatt caatcgagga aacattatct
     7381 gcacttacta aagaagtagc cgacctgcgc gcaaccgcta agggcgaaat tccatcttca
     7441 attcgcgaag aaattgccac acttaccaaa gagaacaatc gcttaaaagg cgaactagat
     7501 caggctgtaa actcagtcga aaaacttcga gccgaaaacg aatccttagt ttctgctggt
     7561 ggcgacgata gcgcccttga agaacttcgg gctaaattaa cccagtcgca aactgaacta
     7621 gctgaagcaa gaaatcagtt agcgaacacc tcgcgaggcc aagaggatct atctggtgaa
     7681 atcgtcagac tcaatagcgc ccttgaagag ttaactgcag ctaatgccaa cttggcaaag
     7741 gagaatgaag agcttaagca agccgctcca gttatggaac ctggcgaagc aagctctgca
     7801 tcagtacgta ttttggaatt ggcgcaacgc acagctgatg aagcagttgc aacagcgcgc
     7861 attgaatctg aaaatctccg caatactgca actgctgaag ttgaacgcct catgcgcgaa
     7921 acaaatgcca acatacaatt gatgactcgc gaatttgaat cacacaaagc aaacatggag
     7981 cgcaaagttg aagaactacg cgcttacgaa cgcgagtatc gcagccgcct tcgcagttac
     8041 ttagaaggtc aacttcgcga acttgaaggt aaaacccttg gtggcgatcg tcaagttgaa
     8101 ggttaatcac ttaacttaaa ataaggaccc gattttcggg tccttttttt ggtgctaaga
     8161 aatatatttt tactggtcag tttttaatta gcactgcttt tagtcagcgt tacttttaac
     8221 aagcatcgcg ctgaagccaa gttcctcatc ggcgacgcta actgtggaaa cggttcccgg
     8281 cgcccaattt gtcgccaaaa cttctgcagc aatgttttga ccatagtttt ccaacgctgc
     8341 cttagtttgg gcatctgtcg cttgccaagt gacactaatg cgatcgctta tgtcaaagcc
     8401 cgatgattta cggccatctt ggattaaccg agttatgtcg cgggcaatac cttgttgaat
     8461 tagctcagga gttagcgcca aatcaagggc tagtgactca ccaccctcag aggcaacagt
     8521 ccagccttct cttggtgctt
//
LOCUS       DBNHVN010000301         2805 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1914659, whole genome shotgun sequence.
ACCESSION   DBNHVN010000301 DBNHVN010000000
VERSION     DBNHVN010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1914659"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..905
                     /gene="murI"
                     /locus_tag="AC16Y1_05760"
     CDS             120..905
                     /gene="murI"
                     /locus_tag="AC16Y1_05760"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIR5831998.1"
                     /translation="MDNRPIGIMDSGVGGLTVVRAILDQLPNEPIIYIGDTANGPYGP
                     KPIAQVRQLALDILDSLVERDVKALVIACNSASAAMLRDARERYSIPVIEVIQPAVRR
                     AVSATRTGHVGVIGTTATINSGAYPDAFAAATHISLTTAACPKFVELVEAGITSGPEV
                     LDVAREYLAPLLQNEIDTLVLGCTHYPLLTGAISLLTGDRVTLVSSAEETAKDVYRTL
                     VENDLLRDPDAQAPYHQFLATGKPFTNLARRFLGPEVNVVESI"
     gene            922..1650
                     /gene="rph"
                     /locus_tag="AC16Y1_05765"
     CDS             922..1650
                     /gene="rph"
                     /locus_tag="AC16Y1_05765"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HIR5831999.1"
                     /translation="MTNRSDGRSADELREVKFTRKWLDHAEGSVLVEFGATRVLCAVS
                     FIEGVPRWKKGSGEGWLTAEYSMLPRATHTRSDRESVKGKLGGRTHEISRLIGRSLRS
                     VIDLKSLGENTIMIDCDVLQADGGTRTAAITGAYVAVVDAVEWARSAGLVNPSAVVLG
                     DSVAAVSVGIVDGQPCLDLHYDDDVRAETDMNVVMTGSGNFIEVQGTAEREPFDRNLL
                     NQLLDLATIGCIELSALQKAALAK"
     gene            1647..2255
                     /gene="rdgB"
                     /locus_tag="AC16Y1_05770"
     CDS             1647..2255
                     /gene="rdgB"
                     /locus_tag="AC16Y1_05770"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIR5832000.1"
                     /translation="MSLKIVLATRNNHKAEELQRILMDQNLSVEILNLSNFPDAPEVI
                     ETESTFEGNALLKARAICNHTGLPAIADDSGLCVNSLNGMPGVLSARWSGTDKNVDEH
                     NLQLVLKQIADVPDVRRGAQFVCKAVAVFPDGTEVIASGTVEGEIVRSPRGTNGFGYD
                     PIFQPLDSQLTTAQMSADQKDQISHRGKALRDLANQLAKVIS"
     gene            complement(2266..2347)
                     /locus_tag="AC16Y1_05775"
     tRNA            complement(2266..2347)
                     /locus_tag="AC16Y1_05775"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2311..2313),aa:Leu,seq:tag)
     gene            complement(2402..>2805)
                     /locus_tag="AC16Y1_05780"
     CDS             complement(2402..>2805)
                     /locus_tag="AC16Y1_05780"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018566878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR5832001.1"
                     /translation="QAVNSALEKVGMLEFADRPPHHLSFGQRRRVALATVLVMNPEIL
                     VLDEPSSNLDPTSRRELADILRSLDITLLMVTHDLPYAYELCERALILSKGQIVADKS
                     TYDILTNQDLMAANRLELPFGFDPRNTPKPA"
BASE COUNT          761 a          616 c          693 g          735 t
ORIGIN      
        1 taacagattg attctgcagg aaattccaaa agcacgctcg ccagagtttt gcaataacgc
       61 gccacctatt aaattccttt aattcattgg ctcgggttca ggttgcctat gcttattcag
      121 tggacaatcg cccaatagga attatggact ccggcgtcgg aggcttaacg gtggttcgag
      181 ctattttgga tcagctacca aacgaaccaa ttatttacat cggcgatacc gcaaatggtc
      241 catatggccc caagccgata gcgcaagtta gacagcttgc gttggatatt ctcgattcac
      301 ttgtcgagcg tgacgttaaa gcgctggtca ttgcctgcaa ctcagccagc gccgcaatgc
      361 ttcgcgatgc tagagagcga tattcgatcc cagttatcga agtaattcaa ccagcagttc
      421 gtagggcagt tagtgcaacc agaactggtc acgttggcgt tattggcact actgcaacaa
      481 taaacagtgg tgcgtaccca gatgcatttg cagccgctac gcacatttca ttgacaactg
      541 ctgcctgccc taaatttgtt gagttagttg aagctggaat aacaagtggc ccagaagttt
      601 tagatgtagc tcgcgaatat cttgcgccac ttttgcaaaa tgaaatagac actcttgtac
      661 ttggctgcac acattatcca ttgctaaccg gtgcgatcag tctgctgacc ggagaccgag
      721 tcacacttgt ttcaagtgct gaagagacag ccaaagatgt ttatcgaact ttagttgaga
      781 atgatcttct ccgtgatcca gatgcgcagg ctccttatca ccaattttta gcaaccggaa
      841 aaccgttcac aaatctggcc cgccgctttt taggtccaga ggtaaacgta gttgagtcca
      901 tttaattgct agagtcgcta cgtgactaat agatctgatg gccgaagtgc cgacgaatta
      961 agagaagtta aattcacccg aaagtggcta gatcatgctg aaggcagtgt gctagtcgaa
     1021 tttggtgcca cccgagtact ttgtgccgtt tcatttattg aaggtgttcc gcgctggaaa
     1081 aaaggcagtg gagaaggttg gttgacagca gagtattcaa tgttgccgcg tgccacgcat
     1141 acccgcagcg atcgggaaag tgtcaaagga aaacttggcg gtcgaaccca tgaaatttct
     1201 cgactaattg ggcgaagcct tcgttccgta attgacttaa agtcattggg cgaaaacacg
     1261 atcatgattg actgtgacgt ccttcaggct gatggtggaa ctcgaactgc agcaattaca
     1321 ggtgcatacg ttgcagtagt ggatgcagtt gaatgggcgc gttctgcagg tttagtaaat
     1381 ccgtcagccg tagtcctagg cgattcagta gctgcggttt ctgttggaat agtcgatggt
     1441 cagccttgtt tagatttgca ctacgacgac gatgttcgtg cagaaacaga catgaatgta
     1501 gttatgactg gtagtggaaa ttttattgaa gttcaaggta cagcagaacg agagccattt
     1561 gatcgaaacc tgcttaatca gctacttgat ttagcaacca taggctgtat cgaactttcc
     1621 gcacttcaga aagcggcact agctaagtga gtttaaaaat tgttttagca actcgcaata
     1681 accataaagc cgaagagttg caacgtattt tgatggacca aaatttatca gtagagatac
     1741 ttaatttaag taattttcca gatgccccag aggtgattga gactgagagc acttttgaag
     1801 gaaatgcatt gttaaaggct cgggcaattt gcaatcacac cggccttcct gcgatcgcag
     1861 atgattcggg cttatgtgtt aactcattaa atggaatgcc cggagtttta tcggcccgct
     1921 ggtcaggtac agataaaaat gttgatgagc acaacctgca gttggtgctc aagcagattg
     1981 ccgacgtgcc tgatgtccgc cgcggagcgc aattcgtctg caaagcagtt gcagttttcc
     2041 cagatgggac cgaagttatt gctagtggca cggtcgaagg tgaaatcgtt cgcagcccaa
     2101 gaggcactaa tggatttggg tatgatccaa tttttcaacc gttagattcg caactaacta
     2161 cggcacaaat gtcagctgat caaaaggatc agattagtca tcgaggtaaa gcgcttaggg
     2221 atttagccaa tcaactagcc aaagttattt cctaagagtc aaacgtgcgg gacgggggag
     2281 ttgaaccccc acacccgaag gcgccagaac ctaaatctgg tgcgtctgcc aattccgcca
     2341 gtcccgctca cgtgcgctct tattctgcca gtaaaccctg ggtaattgaa aatcgagaga
     2401 tttaagcggg cttaggcgta tttcgtgggt cgaagccgaa aggtaactca aggcgatttg
     2461 cagccatcaa gtcttgattc gtcagaatgt cgtaagttga tttgtcggcg acaatttgcc
     2521 ctttcgacag gatcaaagcc cgctcacaaa gttcataggc atatggcaaa tcgtgagtga
     2581 ccattaacag ggtaatgtct agacttcgta aaatatctgc cagttctcgt ctactagttg
     2641 gatcaaggtt tgaacttggt tcatctaaaa caagaatttc cggattcatc actagaactg
     2701 tggcaagagc gacacgcctg cgttgaccga aagataaatg atgcggggga cgatcggcga
     2761 attcgagcat gccaactttt tctagagccg aattcactgc ttgct
//
LOCUS       DBNHVN010000302         3053 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1542484, whole genome shotgun sequence.
ACCESSION   DBNHVN010000302 DBNHVN010000000
VERSION     DBNHVN010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1542484"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1467
                     /locus_tag="AC16Y1_05785"
     CDS             142..1467
                     /locus_tag="AC16Y1_05785"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HIR5832002.1"
                     /translation="MSIPFGFSPNPGDNNDLAAMFENMGRMIRNMGKNTGSVDWDSAR
                     DAVKKTLKSDAAVTDRDEQSMKIATELADLWLNSATSFPQSDTAHRLLTKQQWIDQTF
                     ESWKKIVEPVAQGVSGAMTGLLPNDQDGGSIQIPEEFISSLPPEVAANLRETLASADF
                     AAITGPIMAMAKSMGATMYGTQFGQALGTMANEVLSTTDIGIPLTDHEVSGYVASNVA
                     NFSNSLGLDESDVRIYVALRERAHSRLFANAPWLSSQILSAITAHAKGVKIDTSRIQD
                     AIAGLDPTNPEQIQNSITPDLFEPVLTPEQEKAHEKLEQLLALIEGWVSVVVSQSIAN
                     RLLSAVALDEAFRRRRAAGGPAEKLFAGLIGLELRPKRMREAAQLWEKLTELKGQTDR
                     DDIWQHPDLLPSLSDLGNIQAYLDSQSIDLMTELNRIIETTDAPKEEDS"
     gene            complement(1464..2072)
                     /locus_tag="AC16Y1_05790"
     CDS             complement(1464..2072)
                     /locus_tag="AC16Y1_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjP-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HIR5832003.1"
                     /translation="MTAEQTSLFDDQLYRDSTSSVNKSFRAKLPNPKSLDYEIRRSKR
                     RKKSVGAFRENGKTIVVAPIRMSLKEIEIYAQELIEKLDGHYNKVSSNEILDQRARYL
                     VKNYLEIDLFQSYPVPIDITWVTNQNSRWGSCTPNDGKIRISHRLQLAPQYVIDAVLL
                     HELIHLIVPDHSERFYEYLAKFEDYKLAKEFLAGYSFARENI"
     gene            2508..>3053
                     /locus_tag="AC16Y1_05795"
     CDS             2508..>3053
                     /locus_tag="AC16Y1_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5832004.1"
                     /translation="MEATQDILTSALQLAPSVHVYLRNKDQIQVGLNPQNSLCLPTSL
                     HAIIKKCDGFTSIAKLIELTDRDQDSILQVLNLLVERELLILVNPDIKKLTSNQQSHH
                     LDASRAVNSNAKLVNQRANIRLVIVGGGRLGSTLALLLGNSGFSNLRVLDSNPVTLND
                     LTPWGFSRIDVNLRRDYVIQTM"
BASE COUNT          924 a          630 c          636 g          863 t
ORIGIN      
        1 ctaaattgct tacctaaact ttacggcagt aataattcgc ccgattttgc ccagaattaa
       61 atccgccaaa ggctaaatca caaacaactc cgttggctat atcaacgcgt aatctggagg
      121 tagaaaactt caaggagtcg cgtgtccatt ccatttgggt ttagtccaaa ccctggcgat
      181 aacaatgact tagcagccat gttcgaaaac atggggcgaa tgattcgaaa tatgggcaaa
      241 aacaccggca gtgtggattg ggattctgct cgcgatgcgg ttaagaaaac attgaagtct
      301 gatgcagcgg tcacagaccg tgacgaacaa tcaatgaaaa ttgcaaccga attggctgac
      361 ctctggctaa atagcgctac ttcgtttcca caatcggata ccgcacaccg tctgctaact
      421 aaacaacaat ggattgacca gactttcgaa agctggaaaa aaattgttga accagttgcg
      481 caaggcgttt cgggggcaat gactggtttg ctgccgaatg accaagatgg tggatcaatt
      541 caaatacctg aagaatttat ttcttcactt ccacctgagg ttgccgcgaa tttacgcgaa
      601 actttggctt cagcagattt tgcagcgatc actggaccaa taatggccat ggctaaatca
      661 atgggtgcaa ctatgtatgg tacccaattt ggccaggcac ttggcactat ggccaacgaa
      721 gttttaagta caacagatat cggtattcca ctaaccgacc atgaagtaag tggatatgta
      781 gctagtaatg tggctaattt tagtaactca cttggccttg atgaaagtga tgtgcgaatt
      841 tatgttgcgc tacgcgagcg cgcacacagt cgattatttg ctaatgctcc ctggttaagt
      901 tcacagattc tgtccgcaat cactgctcat gcaaaagggg taaaaattga tacctcccgc
      961 attcaagatg caattgctgg tctcgaccca actaatccag aacaaatcca aaacagtatt
     1021 acgccagatt tatttgaacc agtactaact ccagaacaag aaaaggccca cgagaaacta
     1081 gaacaacttc tagcgcttat cgaaggctgg gtgtcagttg tggttagcca atcaattgcc
     1141 aaccgattac taagtgcggt cgcattagat gaagcgtttc gccgacgccg ggcagctggt
     1201 ggaccagcag aaaagctatt cgccggtttg attggacttg aattaaggcc gaagcgaatg
     1261 cgcgaagccg cccaactttg ggaaaaactt actgaactta aaggccagac cgatcgggat
     1321 gatatctggc aacatcctga cttgttgcca tcactttctg acttaggcaa tattcaggct
     1381 tatcttgatt cacaatcaat cgatttaatg actgaattga atcgaattat cgaaacgact
     1441 gacgcgccaa aagaagaaga ttcttaaata ttttcacgcg cgaatgaata accagctaag
     1501 aattctttag ctaatttata gtcttcaaat ttggccaaat actcataaaa ccgttcactg
     1561 tgatcaggca caatcaagtg aattaactcg tgcagtaaca ctgcatcaat tacatattga
     1621 ggagctaact gtagccggtg tgaaattctg atttttccat cgtttggcgt acatgatccc
     1681 cagcgcgaat tttgattagt tacccaagtg atgtctattg gaactggata actttgaaat
     1741 agatcaattt ctaaataatt tttaacaagg tacctagcgc gttgatcaag tatctcgttt
     1801 gatgaaactt tgttgtagtg cccatccaat ttttcaatta gctcctgggc ataaatttca
     1861 atttctttaa gagacatcct gattggcgct acgacaattg ttttcccatt ttcacgaaat
     1921 gcgccaacag attttttacg tcgtttactt cgtctaattt cgtaatctaa agattttgga
     1981 ttgggcagtt ttgctctaaa tgacttattg accgaactcg ttgaatcccg ataaagctga
     2041 tcatcaaaca atgaggtttg ttcggcagtc atatatttag attatctagc cgcactgacg
     2101 aaactggttt aaaaagagat gaagctctcg agccgcgtgc tcgtccattc gccttcccct
     2161 tgcgaatgcc ggctcgttat ctcgagagct tcaacgaaga atgaatttac gcggtaaatg
     2221 tttacattgc caatgaaatc caataattta ccggtttcac tgtggataag gctggggatc
     2281 acttggggaa aggtatgtga attactgggg attaacggtg taaaacttgg ggatagttaa
     2341 gagacttggg cccaaatatg caggtttatc agggattttg ttatccacgg aatgtggaca
     2401 gaaaatagtt tgtcttaaat ttcagataaa aatttcatgg gcaacttaac ccaaaattgt
     2461 gtgggtaact tgagcgaatt tgactgaatt tgaactacaa ctgcgccatg gaagctacgc
     2521 aagacatcct tacttcggca ctgcaattag cacctagtgt tcatgtctat ttacgaaata
     2581 aagatcaaat tcaagttgga cttaatcccc aaaattcact ctgcctaccg acttcattac
     2641 acgcgataat caaaaaatgc gacgggttta cttcaattgc aaaacttatt gaattgactg
     2701 accgtgatca ggattcgatt cttcaagtac ttaatttatt ggttgaacga gagcttctaa
     2761 ttttggtaaa tcctgacata aaaaaattaa caagcaatca gcaaagtcat cacttagatg
     2821 caagtagagc tgtcaattca aatgccaaat tagttaatca gcgcgcaaat attagattag
     2881 taatcgttgg cggtggacgc ctcggaagta cgctagcttt acttttaggc aactccggat
     2941 tttcaaattt aagagtttta gattcaaacc cagttacttt aaatgattta actccttggg
     3001 gatttagtcg aatcgatgta aatctacgac gcgactatgt cattcaaact atg
//
LOCUS       DBNHVN010000303         1576 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_281603, whole genome shotgun sequence.
ACCESSION   DBNHVN010000303 DBNHVN010000000
VERSION     DBNHVN010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_281603"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="AC16Y1_05800"
     CDS             complement(<1..235)
                     /locus_tag="AC16Y1_05800"
                     /inference="COORDINATES: protein motif:HMM:NF020296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIR5832005.1"
                     /translation="MDRNEKIIKDLRTQLKCEADPEVAIGARAYMRNKFQFIGVKTPE
                     RRRLTRGLLKQSQNFSEKELISLCQDLWNQKERE"
     gene            complement(225..>1576)
                     /locus_tag="AC16Y1_05805"
     CDS             complement(225..>1576)
                     /locus_tag="AC16Y1_05805"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5832006.1"
                     /translation="GGFECTSLSVPLDYDDPYKDAITISVIRLRVSAQPVGSLILNPG
                     GPGGSGIEYARAAEFVVSKVLLDNFDIVGFDPRGVGERSPIECIDDQTTDEYIASDGT
                     PDDQTEIDTSIEILTQFGQDCLTNSPELISNVDTVSAARDIDILRAALGDEKLNWLGK
                     SYGTFLGATYADLFPQNVGRMLLDGAIDPTLSNLQLGEGQARGFEDALARFMEDCLTQ
                     DDCPFNGSVEQGIASVKKILDDLDQIPATLDDGRTFTQAMGVLGVVGGLYDVSYGWAS
                     LRPMLAFALGGDFNALAQSVGFYTSRNDDGSYSDNSNDAIMAINCIDRPDRGTPEEAK
                     VLAEQWSEFAPVFGGYLAWGNIACTYWPVPASGQPKTISAIGSPLILVVGTTHDPATP
                     YVWAQGLAGQLSNAALLTLDGDGHTAYMQGSKCIDEVVDEYFLTGMAENGLVCADGP"
BASE COUNT          441 a          382 c          315 g          438 t
ORIGIN      
        1 attcacgttc tttttgattc cacaaatctt ggcaaagtga gattaattct ttttccgaaa
       61 agttttgaga ctgttttaac aaacccctag ttagtcggcg tctttcagga gttttgacac
      121 caataaattg gaatttattt cgcatatatg cgcgtgcgcc aattgctact tcaggatcag
      181 cctcacactt aagttgcgtg cgtagatcct tgattatttt ctcgttacgg tccatctgca
      241 cagacaaggc cattttcagc cattccagtt aagaaatact catctacaac ttcatcaatg
      301 catttggaac cttgcatgta tgcggtgtgg ccatcgccat ccaaagttag taaagcagcg
      361 tttgaaagtt ggccagccaa accttgagcc catacgtaag gagttgctgg atcatgcgtg
      421 gtgccaacta cgagaattaa cggtgaacca atagctgaaa ttgtttttgg ttgaccgctt
      481 gctggaactg gccaatatgt gcaggcaata tttccccaag caaggtagcc accgaatact
      541 ggtgcaaatt cactccattg ctcggccaag acctttgctt cttcgggtgt gccgcggtct
      601 ggtcgatcta tgcaattgat cgccataatt gcatcatttg agttatcaga ataactaccg
      661 tcatcgttac gactggtata aaaaccaaca ctctgcgcca gtgcgttgaa gtcaccacct
      721 agcgcaaatg ccaacattgg gcgcaagctg gcccaaccat atgaaacgtc atatagtcca
      781 ccaacaacac caagtactcc catggcttga gtgaaagttc ggccatcatc aagtgtggct
      841 ggaatttgat ctaagtcgtc aagaattttc ttaactgaag caattccttg ttcaactgat
      901 ccattaaatg ggcagtcatc ttgggttaag caatcttcca taaacctggc tagcgcatct
      961 tcaaaaccac gagcttggcc ctcacctaat tgcaaattag aaagtgtcgg gtcgattgca
     1021 ccatctaaca acatgcgacc gacattctgc ggaaatagat cagcataagt ggcacctaaa
     1081 aatgtgccat aactttttcc aagccaattc aatttttcat cacccagtgc ggcacgcaaa
     1141 atatctatgt cacgtgcagc actgactgta tcgacattgc tgatcaactc aggtgaatta
     1201 gttaaacaat cttgaccaaa ttgagtaagg atttcaattg aagtatcgat ttcagtttga
     1261 tcgtcaggtg ttccgtcact ggcaatatat tcatcagttg tttggtcatc aatacattca
     1321 attggtgacc tttcaccgac gccgcgtgga tcaaaaccga ctatgtcaaa gttgtctaac
     1381 aaaactttac ttacaacaaa ttctgccgcc cgcgcatatt caatcccgct gccaccaggt
     1441 ccacccggat ttaaaattag cgatccaact ggttgagcac tcacccgaag tcgaatcact
     1501 gaaattgtga tcgcatcttt atagggatcg tcatagtcga gtggcaccga aaggctcgta
     1561 cattcaaagc cgccac
//
LOCUS       DBNHVN010000304         4481 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_429362, whole genome shotgun sequence.
ACCESSION   DBNHVN010000304 DBNHVN010000000
VERSION     DBNHVN010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_429362"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..617
                     /locus_tag="AC16Y1_05810"
     CDS             <1..617
                     /locus_tag="AC16Y1_05810"
                     /inference="COORDINATES: protein motif:HMM:NF013565.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR5832007.1"
                     /translation="LWKHERPGDPSWPTSIGVGRPGWHIECAAIALEYLGETIDVQGG
                     GSDLKFPHHEMSAAHAQSATGKAFAKNYVHTGMVSLNGEKMSKSLGNLVFVSKLCASG
                     VDPMAIRLAILANHYRSDWEWTDQKLNEADARLKKWRAALANEHSAPDVSKEIAELLA
                     NDLATPAVLELIDDWASQTLSDSKLNSKGNQAMQITIDALLGII"
     gene            644..1387
                     /locus_tag="AC16Y1_05815"
     CDS             644..1387
                     /locus_tag="AC16Y1_05815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HIR5832008.1"
                     /translation="MMNEKSEDLKVLGQLANASNATLLVERSGHKYIYKPKSGERELW
                     DFPTGTLYQRERAAYVMSEIFTWDFVPETQIIEGPYGIGSLQDWHDSEITEVDIFLPQ
                     EIPSDWLKIFSGIDEHGQQVVLAHAQNQQLARICLFDAVINNADRKAGHLLTDSVGKI
                     WAVDHGVTFNEEPKLRTVLWGWIGEPIPEALMTDLEKGLHSVKDSELVELLNESEFSQ
                     LINRIEDLIAQGVFPTPNPGWPAVPWPVF"
     gene            complement(1390..1941)
                     /locus_tag="AC16Y1_05820"
     CDS             complement(1390..1941)
                     /locus_tag="AC16Y1_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HIR5832009.1"
                     /translation="MTRIVHLFDNPERFVVGTVGQPGERTFFLQAKQANRLVSVALEK
                     QQVNLLADRMTELLDQVSKLENLQITNQAPADLEPLDNPIEEEMRVGTIALGWNSITA
                     QVVVEAHAVTEFIDDVPELEEDSDEGPDVLRVRMSGSYARSFCLRAKAVVAAGRPNCP
                     LCDNPLDPRGHICPRANGYKRRG"
     gene            complement(1938..2615)
                     /locus_tag="AC16Y1_05825"
     CDS             complement(1938..2615)
                     /locus_tag="AC16Y1_05825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_4193 family putative phosphomutase"
                     /protein_id="HIR5832010.1"
                     /translation="MGTLILLRHGRTDANAQGVLAGRSPGVQLDEVGIKSCDFLREKF
                     SSLDIKHVATSPLERTIQTAAIVFPKNKSRIEPDLTECDYGDWTGKKLSDLVKEPMWE
                     TVRKAPQDVVFPEGESMQAMSDRAINAVLNLDNELTNEYGEEFIWAAVSHGDIIKAIV
                     AHALGLELSKFQRIYVEPASVSVLRIKGDDTGVLKVNDTGDSWVQSLAKVGESTVGGQ
                     SGQESAK"
     gene            complement(2700..3530)
                     /locus_tag="AC16Y1_05830"
     CDS             complement(2700..3530)
                     /locus_tag="AC16Y1_05830"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019766504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR5832011.1"
                     /translation="MTEQLSWLQAIILGISQGLTEFLPISSSAHTLIISKLAGWPDPG
                     AAFTAVSQLGTELAVLIYFRKDLAAIIKSWFSSVFKFQRPQDDHAKLGWYIIFGSIPI
                     AILGVTFKDQIETTARNLYLVSFSLIFMGLLLGLADRLGKHSKDFSNLKTRHALLFGI
                     GQALALIPGVSRSGATITFGLAMGFKREFAARYSFLLAIPAVIGSGLFTARDIGSDDF
                     VNWPATLLATFIAFIVGYFVISALMNYLKTKSFMPFVIYRVVLGSVLLVLLQFGTISS
                     "
     gene            3589..3777
                     /locus_tag="AC16Y1_05835"
     CDS             3589..3777
                     /locus_tag="AC16Y1_05835"
                     /inference="COORDINATES: protein motif:HMM:NF040150.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 family protein"
                     /protein_id="HIR5832012.1"
                     /translation="MSAVWEFREIKFSPTVKRGEIRTLLTTMAEIERWEIDRVRVTHD
                     GRRWIRLRRKTYLVARTA"
     gene            complement(3779..>4481)
                     /locus_tag="AC16Y1_05840"
     CDS             complement(3779..>4481)
                     /locus_tag="AC16Y1_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013425725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5832013.1"
                     /translation="MKLRAEGTAGHGSFLNNDNAVTRLAEAVAAVGNHKFDVALTPTV
                     RDFIKAVSDALGMELSADDPEQLLAQLGPIARIIGATLSNTANPTMLNAGYKHNVIPG
                     VAEAMIDGRFLPGFEDELIKDIENLLPPGVIREDVVNGIALEAPFEGPLVEAMSAAIR
                     AEDPMGTPVPYTVSGGTDAKAFSTLGIKCYGFLPLLLPPELDFSAMFHGVDERVPVTG
                     LEFGVKVMDRFVRSA"
BASE COUNT         1315 a         1015 c          936 g         1215 t
ORIGIN      
        1 ttctttggaa acacgagcgc cctggcgatc caagttggcc aacgtcgatc ggagttggtc
       61 gtccaggttg gcacattgag tgtgctgcta ttgcacttga atacttgggt gaaaccatcg
      121 atgtacaagg aggtggcagt gatttgaaat tcccgcatca cgaaatgtcc gcagcgcatg
      181 cgcaaagtgc aaccggtaaa gcttttgcca agaattatgt gcataccggc atggtctcgt
      241 tgaacggcga aaagatgagt aagtcacttg gcaatttagt ttttgtctca aaactttgtg
      301 caagcggtgt tgatccaatg gcaattcggt tggctatcct ggcaaaccat taccgaagtg
      361 attgggaatg gactgaccaa aaacttaatg aggcagatgc tcgcctgaaa aaatggcggg
      421 ccgcattggc aaatgagcat tccgcacctg atgtcagcaa ggaaattgcc gagttattag
      481 caaacgattt agctacaccc gctgtacttg aattaataga tgactgggct tcccagactc
      541 taagtgatag caaactaaat tcaaaaggta accaggcaat gcaaataaca atcgatgcct
      601 tacttggcat tatttaattt cgggcttaaa aaaattattt caaatgatga atgaaaaaag
      661 cgaagatctt aaagttcttg gacaattagc gaacgcctct aacgcaacat tgttagttga
      721 gcgatctggt cataaataca tctacaaacc aaaaagtggc gaacgtgaac tttgggactt
      781 tcctaccgga actctttacc agcgtgaacg tgctgcctat gtaatgtcgg aaatatttac
      841 ctgggatttt gtgcctgaaa cgcaaattat cgaagggccc tatggaatcg gtagtcttca
      901 agattggcac gatagcgaga taactgaagt tgacatcttt ttaccccaag aaattccctc
      961 tgattggtta aaaatattta gcggcatcga cgaacatggt cagcaagttg ttttagcgca
     1021 cgctcagaat caacaattgg ccagaatctg tttatttgat gcggtaataa ataatgctga
     1081 tcgaaaagct ggtcatcttt taactgattc ggttggaaag atttgggccg ttgatcacgg
     1141 agttactttc aacgaagagc ccaaactgcg aactgtactt tggggttgga ttggggaacc
     1201 aatacccgaa gcgttaatga cagacctaga aaaaggtttg cactcagtta aggacagtga
     1261 actcgtcgag ctacttaatg agtctgaatt ttcccaactt ataaatcgaa ttgaagattt
     1321 aatcgcgcaa ggagttttcc cgactccaaa tcctggttgg cccgcagtac cttggccagt
     1381 tttttaaatt taacctctac gcttgtaacc attcgctcgc gggcaaatgt gcccgcgagg
     1441 atctaacgga ttgtcacaaa gtggacagtt aggacgacca gcagctacaa ctgctttggc
     1501 tcgaaggcaa aaacttcggg catatgaccc actcattcga actctaagta cgtctgggcc
     1561 ttcgtcgcta tcttcttcca gctcagggac atcatcaata aattcagtaa ctgcatgagc
     1621 ctcaacaaca acttgtgcag taatcgagtt ccaaccaagt gcaatggttc caacgcgcat
     1681 ttcttcttca atagggttat ctaaaggttc taggtcggct ggagcttgat tagtgatttg
     1741 caggttttcc aacttagaca cctgatcaag taactcagtc attcgatctg ctagtaaatt
     1801 aacttgctgt ttttctaacg caacgcttac gaggcgatta gcttgtttgg cctgaagaaa
     1861 aaaggtgcgc tcgccaggtt gtccgactgt acctacgaca aatcgctcgg gattatcaaa
     1921 tagatgaact atccgagtca ctttgcgctc tcctgcccac tttgtccgcc tacggttgat
     1981 tcgccaactt ttgctagaga ctgaacccaa ctatcaccgg tgtcattgac tttcagtaca
     2041 ccagtatcat caccctttat tcgtaaaaca ctgacactgg ctggttcgac atatattctt
     2101 tgaaattttg aaagttctag tccgagtgca tgtgcaacta ttgctttaat aatgtcacca
     2161 tggctaacgg cagcccagat aaattcctcg ccatactcat tagttaattc attatctaga
     2221 tttagtaccg catttatagc ccgatctgac atagcttgca tgctttcacc ctcaggaaaa
     2281 actacatctt gaggtgcctt acgaactgtt tcccacatag gttctttaac taaatccgat
     2341 agttttttac cagtccaatc gccgtaatca cattccgtta ggtccggttc gattctggac
     2401 ttatttttag ggaaaacaat tgcagcggtt tgaatagttc gttcaagtgg actcgttgct
     2461 acatgtttaa tgtctaaact tgaaaatttc tcccgcaaga aatcacacga cttaattccc
     2521 acttcatcta gttgaacacc aggactgcgt ccagctaata caccttgggc attagcatca
     2581 gtccgaccat gccgaagtaa aattaatgtt cccacatgaa aagcctattg gtttaataag
     2641 tcgaatgaat cttggttcgc tcagcgctta cccgactcaa ggacagtaac tgtaatgatc
     2701 taactagaga ttgtcccgaa ttgaagtaaa accaataaga cagaacctaa aacaacacga
     2761 taaataacaa atggcataaa ggatttggtt ttcaaatagt tcatcaacgc agatatcacg
     2821 aaatagccca ctataaatgc gatgaaagta gctaataatg ttgccggcca atttacaaaa
     2881 tcatcagatc cgatgtcacg agcggtaaat agcccactac cgataacagc tgggatcgct
     2941 aaaaggaatg aatatcgagc tgcaaattct cgtttgaaac ccatggctag tccaaaagta
     3001 atagttgccc cggaccgact tactcctgga attagcgcaa gcgcttgtcc gataccgaat
     3061 aacagtgcgt gcctagtctt taagttggaa aaatccttag agtgtttacc taggcgatct
     3121 gccagcccaa gcagcaatcc cataaatatg agactgaacg aaactaaata cagatttcgt
     3181 gcagtggttt caatctgatc cttaaaagtc acacctagaa ttgcgattgg gatagaacca
     3241 aaaattatgt accaacctag ttttgcgtgg tcatcttgcg gcctttggaa tttaaacacg
     3301 ctactaaacc aactcttaat aatggctgcc agatcttttc gaaaataaat caataccgcc
     3361 agttcagttc ctaactgact tactgcagta aaagcagcac cagggtctgg ccagccggca
     3421 agtttggaaa taatcaaagt atgtgcggat gaacttatcg gcaaaaattc agttaagcct
     3481 tgagatatgc ccaaaataat tgcttgcaac caagataact gttcggtcac tcaacacgcc
     3541 tatcgttagt tgctctaggt atttagtgta aaaggctaat ctcaatttat gtccgctgtg
     3601 tgggagtttc gtgagattaa gttctcacca acagtaaagc gcggcgaaat tcgtactttg
     3661 ctaacaacca tggcagaaat agaacgctgg gaaatagatc gagtgcgagt aacccatgat
     3721 ggccgacgtt ggattcgact ccgccgtaaa acttacttag tggctcgcac tgcgtaaatt
     3781 atgcagatct aacaaaccga tccataactt tcactccaaa ttccaaacca gtgactggaa
     3841 ccctttcatc aacaccatgg aacatggcgc tgaaatctaa ttccggtgga agtagtaacg
     3901 gtaaaaatcc gtaacactta attcccaagg tgctaaaagc tttagcatca gttccgccag
     3961 aaactgtgta cggaacggga gtacccatcg gatcttcggc tcgaattgct gcggacatcg
     4021 cctctacaag tggaccttca aatggcgctt ctaatgcaat tccatttacg acatcttccc
     4081 gaataacacc tggtggtaat aaattttcga tgtctttaat caattcatct tcaaagccgg
     4141 gcaagaaacg tccatcaatc atcgcttcgg ctacgcctgg aattacgttg tgcttataac
     4201 cagcattcaa cattgttgga ttcgctgtat ttgaaagagt agcgccaatt attcgggcaa
     4261 tcggtcccaa ctgagccaag agttgctcgg gatcatcagc acttaattcc ataccaagag
     4321 catccgaaac cgcctttatg aagtcacgaa ctgttggagt taacgccaca tcaaatttat
     4381 gatttccaac ggcggctaca gcttccgcta atcgcgtaac ggcattgtcg ttattcagaa
     4441 atgacccatg tcctgcggtt ccttcggcgc gcagtttcat c
//
LOCUS       DBNHVN010000305         2747 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1544181, whole genome shotgun sequence.
ACCESSION   DBNHVN010000305 DBNHVN010000000
VERSION     DBNHVN010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1544181"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1679)
                     /locus_tag="AC16Y1_05845"
     CDS             complement(<1..1679)
                     /locus_tag="AC16Y1_05845"
                     /inference="COORDINATES: protein motif:HMM:NF015644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HIR5832014.1"
                     /translation="MSQINLGNPKKSGFGVALTALIVAFVAFSIAAGFYVDWQWFAVL
                     ERTDVYIKELVVKTALFVGVGGLTAFFVWLSASLAYRARPFELPRTPEEFALARYREA
                     LDSFRRAAFVIAPLFFGALIGLSAAGQWRVFLQWQNATPFNQVDAQFGKDISFYVFEL
                     PFYRTILSFAFTALISSLVINLAIHYLYGSLRPTSSIASNVARRHLMIFLGLLASLKA
                     VAYYLDRFALAVKSDSLITGLKYTDVNALIPAKNILTYIAIAVALLFFISLFRPGWQL
                     PFVSFFVMLASSIIIGGLYPAFVQQFQVRPSEFQREAPYIERNLAATKQAYDIADVQI
                     TDYAAIENPSEAVLTEDSATLNNIRILDPSLLSPTFRNLQQIRGFYAFADTLDIDRYL
                     IDGVKRGTVIAVREINLEGVADTQRNWFNDTMVFTHGYGAVAAFENTVSRDGSPAFFE
                     YDVPPTGGLQIDQPRIYFGEQSPNYSIVGKGPNGEDRELDYPDDNSENGQTNNTYDGT
                     GGVAVGNMLQRAIFAVNYQDPNILLSNQMTQDSKILYERNPKDRVAKIAPW"
     gene            1749..2264
                     /locus_tag="AC16Y1_05850"
     CDS             1749..2264
                     /locus_tag="AC16Y1_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPA1309 family protein"
                     /protein_id="HIR5832015.1"
                     /translation="MENVGNLAAVVQEVESHISAAGWDQPIRLFAVVSTADLIASEPA
                     LAAELNLSDSMALTSIEQELSATLNLEELLATIAWPQNVLGAILAIERIVLPPDAQAQ
                     LPSEDEPNWLAEVSNHPLRRDVRIISAVMRNGENLNALRYREHDEPSAVAIAPDLVVN
                     LNESLLATFSD"
     gene            complement(2261..2383)
                     /locus_tag="AC16Y1_05855"
     CDS             complement(2261..2383)
                     /locus_tag="AC16Y1_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004808421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5832016.1"
                     /translation="MCSPVNCPSCGKITWQGCGQHLDEVFAGLSEDQICKCDES"
     gene            complement(2417..>2747)
                     /locus_tag="AC16Y1_05860"
     CDS             complement(2417..>2747)
                     /locus_tag="AC16Y1_05860"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018776488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HIR5832017.1"
                     /translation="IGGPSAGMMFSIAIVDKLTPGAITNGIKIAGTGTIDGDGNVGPI
                     GGINRKLIGASDAGTKLFLAPLENCSEVVGNIPDGLTVVPVATLTQAIKAIADFNSGA
                     TLPQCTS"
BASE COUNT          817 a          608 c          616 g          706 t
ORIGIN      
        1 aaccaaggtg caattttagc tacgcgatcc tttggattgc gttcgtaaag aatttttgag
       61 tcttgggtca tttggtttga taacaagatg tttgggtctt gataattaac ggcaaatatt
      121 gcacgctgca acatgtttcc tacggcaacg ccgccagtgc catcataagt attgttagtt
      181 tggccatttt cactattgtc atctgggtaa tcgagttctc gatcttcacc atttggaccc
      241 ttaccaacta ttgaatagtt aggcgattgc tcaccgaaat aaattcttgg ctggtcgatc
      301 tgtaaaccac cagtaggtgg cacatcgtat tcaaaaaatg ctggtgaacc atcacggcta
      361 actgtgtttt caaatgcagc taccgcgcca taaccatggg tgaaaaccat agtgtcgtta
      421 aaccagttgc gctgagtatc tgcaacaccc tcaagattaa tttcgcgaac agcaattaca
      481 gttccacgtt ttacgccatc aattaaatag cggtcgatat ctagcgtgtc agcaaaagcg
      541 tagaaaccac gaatctgctg taagttgcgg aaagtcgggg ataacaaact cggatctaga
      601 attctgatgt tgttcaaagt tgcagaatct tctgttaaca ccgcttcgga cggattctcg
      661 attgcggcgt agtcagtaat ttgaacatca gcaatgtcat aagcctgctt agtggcagct
      721 aagttgcgtt caatgtatgg agcttctcgc tgaaattcac ttggtcgtac ttggaattgc
      781 tgtacgaagg ctgggtacaa gccaccaatg attattgacg aagccagcat cacaaagaat
      841 gaaacaaagg gtagttgcca acctggtcta aacaatgaga tgaagaaaag tagtgcaact
      901 gcaattgcaa tgtaagtaag gatatttttt gctgggatta gcgcattaac atcggtgtac
      961 tttaacccag taattagcga atcagattta acagctaacg cgaatcgatc taagtaataa
     1021 gcaactgctt taagtgatgc tagtagtccc aaaaaaatca tcaaatgtct gcgggcaaca
     1081 tttgatgcaa tcgaagaagt cggtcgcaaa ctgccataca gatagtgaat agctaaatta
     1141 attaccagcg aggagataag cgcagtaaag gcaaagctta aaatcgtgcg gtaaaacgga
     1201 agttcaaata cgtaaaacga aatatctttt ccgaattgtg catcaacttg gttaaacggt
     1261 gtcgcgttct gccactgcaa aaacactcgc cactgtccag cagctgataa accaattagt
     1321 gcgccaaaaa atagtggcgc aattacgaat gcggcgcgac gaaatgaatc aagagcctcg
     1381 cgataacgag caagcgcaaa ttcctctggc gtacgaggca gctcgaatgg acgggcgcga
     1441 tacgccaaag aggccgaaag ccatacgaag aaagcggtca agccaccaac gcccacgaat
     1501 aacgcagtct taacaactag ttctttgata taaacgtcag tgcgttctaa gacagcaaac
     1561 cactgccagt ccacataaaa acctgcggcg attgagaagg caacgaaggc aactattagt
     1621 gcggttaagg caacgccaaa accgctcttt tttgggtttc cgagattaat ttggctcaca
     1681 cctctaccct acgatgtttc tggccaacat gaattttggc cagaaacata atggggcaaa
     1741 ctaaaaccat ggaaaatgtg gggaatttag ccgcagttgt gcaagaagtt gagtctcaca
     1801 tcagcgcagc aggctgggac caacccattc gattatttgc tgttgtttct acggcggatc
     1861 taatcgcaag cgaaccggcg ttagctgcgg aactaaattt aagcgactca atggctctaa
     1921 cttcgatcga gcaagaatta tcggcaaccc taaatcttga agaactttta gcaacgattg
     1981 cctggccgca aaatgtgctg ggcgcaattt tggcgattga gcgaatcgtg ttgccaccag
     2041 atgcccaagc ccaacttccg agtgaggatg agccgaattg gctagccgaa gtatcgaatc
     2101 atccacttag acgtgacgta cgaatcatta gcgcggtgat gcgtaatggt gaaaacttga
     2161 atgctctgcg ctaccgcgag catgacgagc caagtgctgt tgcaattgcg ccagatttag
     2221 ttgttaactt gaatgaaagt ttgcttgcaa cctttagcga ctagctttcg tcacatttgc
     2281 aaatttgatc ttcagataga ccagcaaaaa cttcgtctaa atgttgacca caaccttgcc
     2341 aagtaatttt tccgcaagac gggcaattaa ctggagagca catatttttc ctaactgatc
     2401 agttaatttt actcgactaa gaagtgcatt gcggcaaggt ggcacccgaa ttgaaatctg
     2461 cgattgcttt tatcgcttga gttaaagtag caaccggaac gacagttaag ccatctggaa
     2521 tattgccaac aacttcactg cagttctcga gtggggctaa aaacaatttt gtaccagcgt
     2581 ctgaggcgcc aataagtttt cgatttattc cgccgattgg accaacattt ccatcaccat
     2641 caatagttcc ggtgccggcg atttttatcc catttgtaat agcgcccgga gtcaatttat
     2701 ccacgattgc gatactaaac atcattccag cgcttgggcc accaata
//
LOCUS       DBNHVN010000306         2590 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1099610, whole genome shotgun sequence.
ACCESSION   DBNHVN010000306 DBNHVN010000000
VERSION     DBNHVN010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1099610"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="AC16Y1_05865"
     CDS             complement(<1..313)
                     /locus_tag="AC16Y1_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR5832018.1"
                     /translation="MGNAKHVFVTGGVASSLGKGLTASSLGNLLTARGLRVTMQKLDP
                     YLNVDPGTMNPFQHGEVFVTNDGAETDLDIGHYERFLDRDLEGFANVTTGQVYSTVIA
                     KE"
     gene            complement(358..2031)
                     /gene="recN"
                     /locus_tag="AC16Y1_05870"
     CDS             complement(358..2031)
                     /gene="recN"
                     /locus_tag="AC16Y1_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR5832019.1"
                     /translation="MLEQLRIKNLGVIRDAVLDFAPGFTVLTGETGAGKTMVFRSLAL
                     LFGGKSDSSLIATGAEQATVEADVVVNADIATRVAELGSELDQNVLILSRQVNSGGRS
                     RAFAGGVSVPAGVISELGEELIAVHGQSDQIRLTKGDQQRLLLDRFAGEKVATVLAEY
                     RALWDKRKNLKNQIELLSSDAGTRATELERLNRILEQVNSLKPQPKEDLELAEEASRL
                     AHSESLYAAAKQAVDGLTGGESGSENVAGILATIRKAVEHESALDPKLAEFAKRLREL
                     EVLVADLAGDLGGYSAAIDASPQRLAYVEDRRAALKAIAREFGTVDDLLAWVSENEPR
                     RAQLAGGDEVIAQLNQELAVLNTQIKEVAAKLTKARQKAADEFAKLVSAELVGLAMAG
                     AKVEFVILEAKLGPFGGDDISLGLSSRPGAPLVAVAKGASGGELSRIMLAVEVVLAKA
                     DPIETFVFDEVDAGVGGAAAVEVGKRLAQLAKTSQVVVVTHLPQVAAFADKHLVISRI
                     AGDEFHQTAVSEVKDSERVTELSRMLAGLSDSDAGTKLAEELLALARAQ"
     gene            complement(2051..>2590)
                     /locus_tag="AC16Y1_05875"
     CDS             complement(2051..>2590)
                     /locus_tag="AC16Y1_05875"
                     /inference="COORDINATES: protein motif:HMM:NF039482.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase catalytic
                     domain-containing protein"
                     /protein_id="HIR5832020.1"
                     /translation="VVVNGSWTVEERTALSVAVVGQDGAKWNSWALNEISIEKAHRER
                     MATLLVSIDGQPVSKWNCDGLLCSTPTGSTAYAFSAGGPVVWPEVEALLVVPVSAHAL
                     FNRPLVVSPKSVIDIAVINGPVVVSADGRRTTEVGDGGKVHVVRDTAPVKFARIKQTP
                     FTERLVAKFELPVQGFQKK"
BASE COUNT          679 a          719 c          528 g          664 t
ORIGIN      
        1 gctctttagc aatcacggtt gaataaacct gaccggtagt tacgttggca aaaccttcaa
       61 ggtcacgatc taagaatctt tcgtagtgac cgatatctaa atcagtttca gcgccatcgt
      121 tagttacgaa aacttcaccg tgttgaaacg gattcatagt gcctggatct acgtttaagt
      181 aaggatcaag cttttgcatc gtcacacgta aaccacgtgc ggttaagaga tttccaagac
      241 ttgaggcggt taaaccttta ccaagagaac tggccacgcc acccgtgacg aaaacatgct
      301 ttgcattacc cacggaagac aaacgtatca ggtatctgcg cgttaacgca cattgaccta
      361 ctgtgcgcgc gccagcgcca ataactcctc ggctaacttg gtgccggcat cagaatcaga
      421 tagcccggcc aacatccgcg aaagttctgt aacgcgttca ctatctttta cttcactaac
      481 tgctgtctga tggaattcat caccggcaat gcgcgaaata actaaatgtt tatcggcaaa
      541 cgcagcaact tgcggtaaat gtgtgaccac tacgacctgg ctggttttag ccaattgcgc
      601 caaccgttta ccaacttcaa ccgcagctgc cccaccaacg ccagcatcca cttcatcaaa
      661 cacaaaggtt tcaattggat cggctttagc caacacaacc tcaacggcaa gcattattcg
      721 acttaattca ccacccgatg cacctttagc cactgcaaca agtggtgccc caggtcgact
      781 ggataaacca agtgagatgt catcgccacc aaaaggccca agtttggctt caagtatcac
      841 gaactcaact ttggcccctg ccatggctaa accaacaagc tcagcactta ctaattttgc
      901 gaactcatcg gcagcttttt gtctggcttt agttaatttg gctgccacct ctttaatttg
      961 cgtattaagt acagccaact cttggtttaa ctgagcaata acctcgtcgc caccagccag
     1021 ttgggcccgg cgcggttcgt tttcgctaac ccaagccaac aggtcatcaa ctgtgccaaa
     1081 ttcgcgcgcg attgccttta gcgctgctcg gcgatcttcg acatatgcca accgctgcgg
     1141 tgaagcatca atagccgcgc tgtagccacc taggtcacca gctagatcgg ctactaaaac
     1201 ttctagttcg cgaaggcgct ttgcaaactc agccaacttt ggatcaagtg cgctttcgtg
     1261 ttcaactgct ttgcggattg tggccaaaat acccgccaca ttttcactgc cactttcacc
     1321 accagttaaa ccatcgactg cctgcttggc cgcggcataa agtgattcac tatgggccaa
     1381 tctgctggct tcttcggcta actcaagatc ttctttaggt tgaggtttaa ggctattaac
     1441 ttgttccagg attcgattta gccgttctag ttcggtagcc cgtgttccgg catcagaact
     1501 tagtaactca atctggtttt ttaagttttt tcgcttatcc caaagtgctc gatactcggc
     1561 caatactgtg gcaacttttt cacctgcaaa tcgatcaagt aatagccgct gctgatcacc
     1621 tttagtcagt cgaatttgat cactttggcc atgaacggca attaattcct cacccaattc
     1681 agaaataacc ccagctggca ctgatacccc accagcaaag gcccgtgagc gcccaccgga
     1741 attaacttgg cgagacagta ttaatacgtt ttgatctagt tcactaccaa gttcggcaac
     1801 ccgggtcgca atatcagcat taacaactac atccgcttca acagtggctt gctcggcgcc
     1861 agttgcaatc agtgaactat cagatttgcc accaaacaat agggctagcg agcgaaacac
     1921 catagtttta ccagcaccgg tttcgccagt taacaccgta aagcctggtg cgaaatctaa
     1981 aacggcgtcg cgaattacac ccagattctt aatccgcagt tgttctagca cgtagatacc
     2041 ttaatcggaa ttactttttt tggaagcctt ggaccggcaa ttcaaactta gccactaaac
     2101 gttcagtaaa tggagtttgt ttaattcggg caaacttaac tggcgcagta tctcgaacca
     2161 cgtgcacttt gcccccgtcg ccgacttcgg ttgtgcgccg accatcagcg ctaaccacaa
     2221 ctggtccatt gattaccgca atatctatta ctgacttcgg ggaaacaact aaaggtctat
     2281 taaataaggc atgtgcacta actgggacca ccagtagcgc ttcaacttcg ggccaaacta
     2341 ctggcccgcc agcactaaat gcgtacgccg tcgaaccagt tggcgttgaa cacagtaacc
     2401 cgtcgcaatt ccattttgat actggttgcc catcaatact tactaataag gtagccatcc
     2461 gctcgcgatg tgctttttca atcgaaattt cgttcagggc ccatgaattc cacttggccc
     2521 catcttgacc aacaactgcc actgaaagcg cggtacgttc ttcaactgtc cacgagccat
     2581 tcaccacaac
//
LOCUS       DBNHVN010000307         2598 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_206708, whole genome shotgun sequence.
ACCESSION   DBNHVN010000307 DBNHVN010000000
VERSION     DBNHVN010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_206708"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1739)
                     /gene="qcrB"
                     /locus_tag="AC16Y1_05880"
     CDS             complement(66..1739)
                     /gene="qcrB"
                     /locus_tag="AC16Y1_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc1 complex cytochrome b subunit"
                     /protein_id="HIR5832021.1"
                     /translation="MSTTKPNSAIDASSKSVVGGVAGYVDDRLASANFTKRILNKVFP
                     DHWSFMLGEIALYSFIILLLSGTYLTLWFKPSMIEIVYNGSYVPLKGVKMSEAFASTL
                     EISFDVRGGLLMRQIHHWAALFFVAAMSVHVMRIFFTGAFRKPREINWVIGTFLLVLG
                     ILEGFAGYSLPDDLLSGTGLRIAEGIIQAVPLVGSYLAMFIFGGEFPGTDWVARLYAI
                     HILIVPGILLALVTVHLIMVFYQKHTQFPGQGRTESNVVGYRLYPIYIAKAGGFFFIV
                     FGVITLISALVTINPVWLYGPYTPSQVTAGSQPDWYIGWLDGAVRIMPNWETNLLGYT
                     LSWNLIVPALVIPGIMFTALGLYPWIEAWATGDKREHHLLDRPRNAPTRTGLGVMAIS
                     FYLMLWISGGNDVIATAFDLSINAITWSFRVGIFVVPVIAFKVTKRICLGLQRRDREK
                     LLHGYETGQILRLPHGEFIEIHKPVSDAEKAVLLAKQDYTPLQLDAAVDANGVRNPKS
                     RKARLQARISKWYYDENIARPTAAELEAAAHHAEHELEQAQGQVKELSR"
     gene            complement(1736..>2598)
                     /gene="qcrA"
                     /locus_tag="AC16Y1_05885"
     CDS             complement(1736..>2598)
                     /gene="qcrA"
                     /locus_tag="AC16Y1_05885"
                     /inference="COORDINATES: protein motif:HMM:NF050121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome bc1 complex Rieske iron-sulfur
                     subunit"
                     /protein_id="HIR5832022.1"
                     /translation="ILSSIVAYIAIPNDSRVNVPLLGLVNGNHVAVGFFFGLGTFLIG
                     AGAIHWAKKLMPDDEIVQERHPLTSSAADRAEVAEIYKRGAAESGIGERKIIRRTLLG
                     ALSVFPLPFIVFLRDLGPLPGTKLRETIWSQGIHVVTDPTYRKIKAEDIPIGTLINAV
                     PENLLEIEHEEGNLNQRGKASIILVRMDPADIVAQQGEGWDYQGILAYSKICTHVGCP
                     TSLYQQRTHHLLCPCHQSTFDLSDAGNVVFGPAARRMPQLPITVDSEGYLVAKSGFQE
                     PVGPSFWERG"
BASE COUNT          752 a          611 c          590 g          645 t
ORIGIN      
        1 tggttgcaac attagtggtt taaacaaaaa accccggccc aagggtcggg gttttttgta
       61 gttatttaac gcgacaactc tttaacttga ccttgagctt gttctaactc atgttctgcg
      121 tggtgtgcag cggcttcaag ttcggcagca gtcggtcgag cgatgttttc gtcgtagtac
      181 cacttagaaa tacgtgcctg caatctagcc ttgcgactct ttggatttcg aactccattg
      241 gcatctacag cggcatcaag ttgtagtggg gtgtaatcct gcttagcaag gagcacagcc
      301 ttttctgcat ctgataccgg cttatggatt tcaataaatt caccatgagg caggcgcaga
      361 atctgaccag tttcgtaacc atgtagaagt ttctcgcggt cacgtcgttg taaacctaga
      421 caaatacgct ttgttacttt aaacgcgata actggaacca caaatatgcc tacccggaac
      481 gaccaagtaa ttgcattaat cgacaagtcg aatgcggtag caataacgtc attaccacct
      541 gaaatccaaa gcattaagta gaagctgatt gccatcacac caagtccagt gcgagtcgga
      601 gcgttgcgcg gacggtctag taagtggtgt tcacgcttgt caccagtagc ccaagcctcg
      661 atccacggat ataaaccaag tgcggtgaac ataatgcctg gaataactaa agccggcaca
      721 attaagttcc aactaagcgt gtagcccaaa agattcgttt cccagttcgg cataattcga
      781 accgcaccat ctagccatcc gatgtaccag tctggttgcg aacctgctgt tacttgcgaa
      841 ggcgtgtaag gaccgtaaag ccaaaccggg ttaattgtga ctaatgcaga gatcaaagtg
      901 atcacaccaa acacaataaa gaagaagcca ccagctttag caatgtaaat cggatataag
      961 cggtagccga ctacattact ttcggtgcgt ccttgccctg ggaattgtgt gtgcttttgg
     1021 tagaacacca tgatgaggtg aacagttacc aacgccaaca aaataccggg cacgatcaaa
     1081 atgtggattg cgtataacct ggcaacccaa tctgttcctg ggaattcgcc accgaaaata
     1141 aacatggcta agtaggagcc aaccagtggt actgcctgaa taattccttc agcaatccgc
     1201 aagccagtac cagaaagtaa atcatctgga agtgagtagc cagcaaaacc ttctaagata
     1261 ccgagaacta aaaggaaagt tccgataacc cagttgattt cacgaggctt gcggaatgca
     1321 ccggtaaaga aaatgcgcat cacatgcacc gacattgcgg ccacgaaaaa tagtgcagcc
     1381 cagtggtgaa tctgtcgcat taacaatcca ccacgcacat cgaatgaaat ttcaagagtc
     1441 gaagcgaatg cctcggacat ctttacacct tttaatggaa catatgaacc gttataaaca
     1501 atttcgatca tcgatggctt gaaccaaaga gtcagataag ttccagacag tagcaaaata
     1561 ataaaactgt aaagtgcaat ttcaccgagc ataaatgacc agtgatctgg aaatacctta
     1621 ttaagaattc gcttagtgaa attagcggaa gctaaccgat catcaacgta gccagcaact
     1681 ccaccaacta cagatttact cgacgcatca atcgccgagt ttggttttgt tgtgctcatc
     1741 cacgctccca gaagctaggt cctactggct cttggaaacc tgacttggcg accaaatagc
     1801 cttcgctgtc tacagtaata ggaagttgcg gcatccgcct ggcggcggga ccaaagacaa
     1861 cattgccagc atcagatagg tcgaatgtcg attggtggca tgggcagagc aggtgatggg
     1921 tacgctgctg gtaaagcgat gttggacagc caacatgcgt acaaatcttt gagtacgcca
     1981 aaataccttg gtaatcccag ccttcacctt gctgggcaac aatgtcagcc gggtccatcc
     2041 gaacaagaat tatcgaggct ttaccgcgtt ggttcagatt gccttcttcg tgctcaattt
     2101 ctaaaagatt ttctggaacc gcattgatca gtgtgcctat tgggatatct tcggccttaa
     2161 tttttcgata tgtcggatcg gtcacaacat ggataccttg gctccagatt gtttcgcgca
     2221 attttgtacc gggtagcgga cctaagtcgc gcaagaaaac aatgaatgga agtggaaaca
     2281 cacttagggc acctagcagt gttcgtctaa taattttgcg ctcaccaata ccagattcag
     2341 ctgcaccgcg cttgtaaatt tctgctactt cagcccgatc tgctgcgctt gaagttaggg
     2401 gatggcgttc ttgaacgatt tcgtcatctg gcattaactt ctttgcccaa tggattgcgc
     2461 cagcgccgat taagaaagtt cctaaaccaa aaaagaatcc aactgcaacg tggttaccgt
     2521 taactagtcc aagaagtgga acgttaactc gggagtcatt tggtatcgca atataagcaa
     2581 cgatcgaact taagattg
//
LOCUS       DBNHVN010000308         1258 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_61606, whole genome shotgun sequence.
ACCESSION   DBNHVN010000308 DBNHVN010000000
VERSION     DBNHVN010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_61606"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="AC16Y1_05890"
     CDS             <1..719
                     /locus_tag="AC16Y1_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009719810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR5832023.1"
                     /translation="GGERWLQGTFDRHARTVGQAPGYTSIVGSGAHAPILHWVRADGE
                     VHPDELLLLDMGVEVRSGYTADVTRTIPASGKFSSAQKQVHDLVEKSHRAGIASVQPG
                     RLWADFHAACMEVLALGLHDWGMLPVSVDEALSENGQHHRRYIVCGVGHYLGLDVHDC
                     DSSRYETYLGGKLEPGMALTVEPGLYFHAWDETVPPELRGIGVRIEDDLVVTESGHEV
                     ISSALPIDAAGIEAWMASNS"
     gene            747..>1258
                     /locus_tag="AC16Y1_05895"
     CDS             747..>1258
                     /locus_tag="AC16Y1_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5832024.1"
                     /translation="MGHRHAHAKPGETFDAWVDKRRERRVHNFVLFFLIPVTILGIFN
                     IFRTWPDDVAKFDFSSLGYVQETQFYSAEVRTFETFDCIANGATQLPDGTFSTSLCAN
                     VSAELLDGPDKGKVVSFEANASIVQFGIKYGDEVSIASIPAGSETIYVFNDFERTNGL
                     LYLFFVVVVVL"
BASE COUNT          322 a          275 c          317 g          344 t
ORIGIN      
        1 ccggtggcga acgctggcta caaggcacct ttgatcgaca tgcccgcaca gttggacagg
       61 ctcctggtta caccagcatt gtcggatcag gcgcgcatgc cccgatactg cactgggttc
      121 gcgcagatgg cgaagtgcac ccagatgaat tactgctact ggatatgggt gttgaggtac
      181 gcagtggtta cacagcagat gtgacaagaa caattccagc gagcggaaaa tttagtagtg
      241 cccaaaagca agttcacgac ttagttgaaa agtcacatcg cgctgggata gcttcagttc
      301 aacccggccg actatgggca gattttcatg ctgcttgcat ggaagtactt gctctaggct
      361 tacatgactg gggcatgcta cccgtttcag ttgatgaagc actttccgaa aatgggcagc
      421 atcatcgccg ttacatcgtg tgtggtgttg gacattattt gggactagat gtgcacgact
      481 gtgattcatc gaggtatgaa acatatctgg gcggaaagtt ggaaccaggt atggcattaa
      541 ccgtggagcc tggcctttac ttccacgctt gggacgaaac cgtaccgcca gagttacgtg
      601 gaatcggtgt tcgcattgaa gatgatttag tagtaaccga aagcggacac gaagtaattt
      661 cttcagcatt gccaatagat gcagcaggaa ttgaagcctg gatggcaagt aatagctagt
      721 tcataacagc accgtattct tgctgtatgg gacatcgcca cgcacatgct aaacctggtg
      781 aaacctttga tgcctgggtg gataaacgca gagagcgccg agttcacaat ttcgttttat
      841 tctttctaat tccagttacg atccttggaa ttttcaacat tttccggact tggccagatg
      901 acgttgccaa atttgatttc agttctttag gttacgttca agaaacgcag ttttattctg
      961 cagaagtaag aactttcgaa actttcgatt gcatagcaaa tggggccacc caacttcccg
     1021 atgggacatt ttcaacatca ctttgtgcga atgtttcagc tgaactttta gacgggcccg
     1081 ataaaggcaa agttgtgtca tttgaagcga acgcatcgat cgtgcaattt ggcattaaat
     1141 acggtgatga ggtctcgatc gcgtcaattc ccgcaggttc ggaaactatc tacgttttca
     1201 atgactttga gcgaaccaat ggcctacttt atttattctt tgtagtggtt gtagttct
//
LOCUS       DBNHVN010000309         5057 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_61679, whole genome shotgun sequence.
ACCESSION   DBNHVN010000309 DBNHVN010000000
VERSION     DBNHVN010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_61679"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="AC16Y1_05900"
     CDS             <1..439
                     /locus_tag="AC16Y1_05900"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HIR5832025.1"
                     /translation="DLYAPGGRLLSKNTVTLYEDVQEFAVAEINGQIVGCGALHVIWE
                     DLGEVRTIAVLPEHKGKGIGGKILTMLEDKAKELGLKRLFCLTFETDFFGRHGFNEVV
                     EAPVDPTTYAQLLLSGDEGVAEFLDLERVKPNTLGNTRMLKVF"
     gene            583..3072
                     /locus_tag="AC16Y1_05905"
     CDS             583..3072
                     /locus_tag="AC16Y1_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008743301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR5832026.1"
                     /translation="MFERFTDRARRVVVLAQEEARMLNHNYIGTEHILLGLIHEGEGV
                     AAKSLESLEISLEAVRAQVEEIIGQGQQAPSGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLVKLGADLNRVRQQVIQLLSGYQGKEPQVAGGPAEGT
                     PSTSLVLDQFGRNLTQAAREGHLDPVIGREKEIERVMQVLSRRTKNNPVLIGEPGVGK
                     TAVVEGLAQNVVKGDVPETLKDKQIYSLDLGALVAGSRYRGDFEERLKKVLKEIKTRG
                     DIIIFIDEMHTLVGAGAAEGAIDAASILKPMLARGELQTIGATTLDEYRKYIEKDAAL
                     ERRFQPIQVAEPSIAHTIEILKGLRDRYENHHRVTITDGALVNAATLADRYISDRFLP
                     DKAIDLIDEAGARLRIRRMTAPPDLRDFDDKIANVRREKESAIDGQDFERAASLRDDE
                     KNLISARAQREKEWKAGDLDVVAEVDDDLIAEVLAASTGIPVFKLTEEESQRLLKMED
                     ELHKRVIGQQQAIKALSQAIRRTRAGLKDPKRPGGSFIFAGPSGVGKTELSKTLAEFL
                     FGDEDSLIQLDMSEYSEKHTVSRLFGSPPGFVGYEEGGQLTEKVRRKPFSVVLFDEVE
                     KAHPDIFNSLLQILEDGRLTDAQGRVVDFKNTVIIMTTNLGTRDIAKGVSLGFADGSD
                     TSTSYDRMKAKVQDELKTHFRPEFLNRIDDIVVFHQLSRDEIVQIVDLMIDRLDARLK
                     DKDMAIELTTAAKQLLAKKGYDPVLGARPLRRTIQREIEDPLSEKMLFGDLKPGEIVI
                     VDVEGEGEEATFSLRGEFKGELSDIQIETAG"
     gene            3072..3353
                     /locus_tag="AC16Y1_05910"
     CDS             3072..3353
                     /locus_tag="AC16Y1_05910"
                     /inference="COORDINATES: protein motif:HMM:NF022184.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2516 family protein"
                     /protein_id="HIR5832027.1"
                     /translation="MFSGFIWEIAGWIALGVKILALVDVPRRPAEAFPFLNRLTKNAW
                     LAIIVGSVAAHLVFGAWGLTGIIGLIACGIYLADLKPKIDEMQSNSGRY"
     gene            3369..4208
                     /locus_tag="AC16Y1_05915"
     CDS             3369..4208
                     /locus_tag="AC16Y1_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018488960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5832028.1"
                     /translation="MSGVVGDITRGTTNPNRLRRVDRWAYYAICGWIRRCPDPLVVDL
                     GYGATPITTVEMLDRYRKNIRPDIEVVGIEIDPQRVLEAKALEKPGLSFIHGGFEIPT
                     GARKPMMIRAFNVLRQYDESEVTDAWQTMTNRLATDGFLVEGTCDEIGRRSVWVSLRA
                     GQSEPDTITFSTHVETLDKPSDLAPRLPKVLIHRNVPGEKIYDFLQAFDRAWLAKSSL
                     IPFGAKQRFVAAVELLAQEYPIMDNKARWKLGEVTVPWDVVMNTDRPRTPYQTTSCEL
                     PLR"
     gene            complement(4213..4959)
                     /locus_tag="AC16Y1_05920"
     CDS             complement(4213..4959)
                     /locus_tag="AC16Y1_05920"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018559609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HIR5832029.1"
                     /translation="MSEYTLVLVRHGESQWNALNLFTGWVDVDLNEKGLKEAHRAGEL
                     MKSKNVLPDVLHTSLLRRAIKTANIALDVADRHWIPVYRSWRLNERHYGALQGKDKAQ
                     TLAEFGEEQFMLWRRSYDVPPPEINPDSEFAQTYDPRYADIPSSERPATECLADVVKR
                     MLPYWESNIVPDLKAGKVVMVAAHGNSLRALVKHLDGISDDEIAGLNIPTGIPLVYKL
                     DSNLKPTFKEYLDPEAAVAAAAAVANQGKK"
BASE COUNT         1403 a         1117 c         1180 g         1357 t
ORIGIN      
        1 tgacctttac gctccaggcg gccggttact ttcaaaaaat acggtaactc tgtacgaaga
       61 tgtacaagag tttgctgtcg ccgaaattaa tggccaaata gttggttgcg gtgcattgca
      121 cgtgatctgg gaagacttag gtgaagttcg aacaattgcc gtgttaccag aacataaagg
      181 taaaggtatt ggtggaaaaa ttctgaccat gctcgaggac aaggcaaaag aacttgggct
      241 caagcgacta ttttgtttga cttttgaaac cgactttttt ggccggcacg gatttaatga
      301 agtagttgaa gcaccggttg atccaactac gtatgcgcag ctgttgctct ctggtgacga
      361 aggcgtggcc gagtttttgg acttagaacg cgtgaagcca aatacgcttg gaaataccag
      421 gatgttaaag gttttctagt tcgcctaacg cgtaggccaa tggctaaaaa ccccgatttt
      481 tgggaacagt tagcacctat attcagttga aataactaac cgagaagccg agtttcgcat
      541 ctggctctag gctaagaaaa agcgacattc aaggagtttt tgatgttcga aaggtttacc
      601 gaccgagccc gacgcgtagt agtgcttgcc caagaagagg cacgcatgct caatcacaac
      661 tacatcggca ctgaacacat tctgcttggc ctgattcacg aaggtgaagg cgtcgctgca
      721 aagtcactcg aatcactcga gatatcactt gaagcagtac gtgctcaagt tgaagaaatt
      781 attggccaag gtcaacaggc acctagtggt catattccat ttaccccacg cgctaagaaa
      841 gtgcttgaac tttcacttcg cgaagctttg caactaggcc ataactacat cggcaccgaa
      901 cacattttgc ttggtttaat tcgtgaaggt gaaggcgttg ccgcgcaggt attagtaaaa
      961 cttggcgctg acttaaaccg cgttcgtcaa caagttattc aattactttc tggctaccaa
     1021 ggtaaagaac cacaagtagc aggtggcccg gctgaaggaa ctccgtctac ctcacttgtg
     1081 cttgatcaat tcggtcgcaa tttaacccaa gctgcccgcg aaggccatct tgatccagtt
     1141 atcggtcgag aaaaagaaat agaacgcgta atgcaggttc tttctcgtcg caccaagaac
     1201 aacccagtat taataggtga accaggtgtt ggtaaaactg cagttgtcga aggcctggcc
     1261 caaaatgttg ttaaaggcga tgtgccagaa actcttaaag acaaacagat ttattcactt
     1321 gatcttggcg cattagttgc tggttctcgt taccgcggtg atttcgaaga gcggttaaag
     1381 aaagttctta aagaaattaa aactcgtggc gacatcataa ttttcattga tgaaatgcac
     1441 accctagttg gcgccggtgc tgctgaaggt gcaatcgatg cggccagtat tttgaagcca
     1501 atgttggcgc gcggtgaact acagacaatc ggtgccacca cccttgacga ataccgcaag
     1561 tacattgaaa aggatgcagc gctagaacgt cgcttccaac caattcaagt tgctgagcca
     1621 tcaattgcac acactattga aattcttaaa ggccttcgcg atcgttatga aaaccaccac
     1681 cgagttacca tcactgatgg tgcattagta aacgcagcaa cattggctga ccgttacatc
     1741 agtgatcgct ttctgccgga caaggcaatc gacttaatcg atgaagctgg tgctcgtctt
     1801 cgcatccgtc gcatgaccgc accaccagac ttacgtgact ttgatgacaa gatcgcaaat
     1861 gtccgtcgcg aaaaagaatc tgccattgat ggtcaagatt ttgagcgtgc tgcatcgcta
     1921 cgtgatgacg aaaagaattt gatttctgct cgcgcccagc gcgaaaagga atggaaagcc
     1981 ggcgatctag acgtagtggc tgaagttgat gatgacttaa tcgctgaagt acttgcagct
     2041 tcaaccggta tcccagtctt caaattaact gaagaagaat cacagcgtct attaaagatg
     2101 gaagacgaac ttcacaagcg agttattggt caacaacagg cgatcaaggc actttcgcaa
     2161 gcaattcgtc gtacccgcgc aggacttaaa gatccaaagc gtccgggtgg atcgttcatt
     2221 ttcgctggtc catctggtgt tggtaagact gaactttcta agactctcgc tgaattctta
     2281 ttcggtgatg aagattcatt aattcagttg gatatgtccg aatactcaga gaagcacaca
     2341 gtttctcgcc tattcggctc acctccagga ttcgtcggtt acgaagaagg cggacagtta
     2401 actgagaagg tacgtcgtaa accatttagc gttgtacttt tcgatgaagt tgaaaaagca
     2461 cacccagata tctttaactc actacttcaa attcttgaag atggtcggtt aactgatgcg
     2521 caaggtcgag tagttgattt caagaacacc gtaatcatca tgaccacaaa ccttggtact
     2581 cgtgacattg caaagggagt cagcttaggt ttcgctgatg gatctgacac ttcaacttct
     2641 tacgatcgca tgaaggctaa agttcaggat gaattaaaga ctcacttccg tccagaattc
     2701 cttaaccgta ttgatgacat cgtggtcttc caccagttaa gtcgcgatga aatcgttcaa
     2761 atcgttgatt tgatgatcga tcgtcttgat gctcgcctta aagacaagga catggctatc
     2821 gagctaacaa ctgccgcaaa gcagttacta gctaaaaagg gttatgaccc agtacttggt
     2881 gcccgtccat tgcgtcgcac aattcaacgt gagattgaag atccgttaag cgaaaagatg
     2941 cttttcggtg atctcaagcc aggcgaaatt gtgattgtcg atgttgaagg cgaaggcgaa
     3001 gaagcaactt tctcgctacg cggcgaattc aaaggtgaac tatctgacat tcagatagag
     3061 actgctggct aatgttttcc ggattcattt gggaaattgc tggttggatc gcccttgggg
     3121 taaaaatact tgccctcgtt gatgtaccaa gacgaccggc cgaagccttt ccatttttga
     3181 atcgcctaac taaaaatgct tggttagcga tcattgttgg tagcgtcgca gcacacttag
     3241 tgtttggcgc ttggggacta accggaatta ttggcttaat tgcttgcgga atttaccttg
     3301 ctgatctcaa accaaaaatt gatgaaatgc aaagtaattc tggtcgctac taaggcataa
     3361 gtaattgcat gagcggcgta gttggcgaca tcactcgcgg cacaacaaat ccaaatcgct
     3421 tgcggcgggt tgatcgctgg gcctattacg caatttgtgg ttggattcga cgttgcccag
     3481 atccattagt agttgactta ggttacggcg caacaccaat aacgacagtt gaaatgcttg
     3541 atcgctatcg aaaaaacatt cgaccagaca ttgaagttgt tggaattgaa atagatccac
     3601 agcgcgtact ggaagctaaa gcgcttgaaa aacctggttt aagtttcatt cacggtggtt
     3661 ttgaaattcc aactggtgct cgaaaaccaa tgatgattcg agcgttcaat gtgttgcgtc
     3721 aatacgacga atctgaagtt actgatgctt ggcaaactat gactaatcgg ttggcaacag
     3781 atggttttct agttgaaggc acttgtgacg aaattggtcg ccgaagtgtt tgggtaagtt
     3841 tgcgggctgg tcaaagtgaa ccagacacga ttactttttc aacccacgtt gaaacattag
     3901 ataaaccaag tgacttagca ccgcgtctac ctaaagtttt gatccatcga aatgtgccag
     3961 gcgaaaaaat ttatgacttt ttacaagcat ttgatcgggc ttggttagca aaaagtagcc
     4021 tgattccatt tggtgccaag cagcgatttg tagccgcggt tgaattactc gcgcaggaat
     4081 atccgattat ggataacaaa gctcgctgga aactcggtga agttacggtt ccttgggatg
     4141 tagtcatgaa tacagatcga ccacgaactc cgtatcaaac aacatcttgc gagttaccgc
     4201 ttaggtagct tgttacttct taccttggtt agccacggct gcagcggccg caactgcagc
     4261 ctctggatct aaatactctt taaatgttgg ctttaagttg ctatctagtt tgtaaacaag
     4321 cggaatacca gtgggaatgt ttagtccagc gatttcatca tcgctgattc catcgagatg
     4381 ttttacaagt gcgcgaagtg agttaccgtg cgcagcaacc attacgactt tgccagcttt
     4441 taagtccggc acaatatttg attcccaata aggcaacatg cgcttaacca catcggcaag
     4501 acactcggtt gctgggcgtt cacttgatgg aatatcggca taacgcggat cataagtctg
     4561 cgcgaattca ctatctggat ttatttctgg tggtggcaca tcgtatgagc gacgccacaa
     4621 cataaattgt tcttcaccaa attcagccag agtttgcgcc ttatctttac cttgaagtgc
     4681 gccgtaatgc cgttcattca atcgccaact gcgataaacc ggaatccagt ggcgatcggc
     4741 aacatcgagt gcgatgtttg ctgtttttat tgctcgacgt aagagtgaag tgtgtaatac
     4801 atctggcaac acattttttg acttcattaa ttcgccagcg cgatgtgctt ctttcaaacc
     4861 cttttcattt aggtcaacat caacccaacc ggtaaaaaga tttaacgcat tccattggct
     4921 ttcaccatgt cgaactaaaa ccagggtgta ttcactcata acttaattct gccttagtca
     4981 ttgaatagat gtgcaaacgc ttgcagattg gcagtgtcca ttccattact agtccgccaa
     5041 gcccattcac gttcgat
//
LOCUS       DBNHVN010000310         8789 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1397519, whole genome shotgun sequence.
ACCESSION   DBNHVN010000310 DBNHVN010000000
VERSION     DBNHVN010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8789)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8789
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1397519"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="AC16Y1_05925"
     CDS             <1..941
                     /locus_tag="AC16Y1_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005626428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR5832030.1"
                     /translation="FLRKAGDVIPEILGPVVELRDGNEKAFKMPKKCPECNSPLAPEK
                     ESDVDIRCPNSQSCPAQLRGRLEHLGSRSVLDIEGLGEKAARALLEDKIINDEGDLFA
                     LTEAKLKKSDFFVRGANREFAENGKLLLAQIELAKTKPLWRILSALSIRHIGSTTAQA
                     IANEYSSIDQLAKAKVEEIAQVEGIGQTIAQAIVEWFSEPWHRDVVEKWRKAGVQLAQ
                     EKIVTGPQILAGLTLVVTGSLEKYSREGAQEAITSRGGKCASSVSKNTDFVVIGPGAG
                     SKAKKAEELGRPILDEAGFDLLLTSGPEAVKAKYFS"
     gene            1011..1307
                     /gene="gatC"
                     /locus_tag="AC16Y1_05930"
     CDS             1011..1307
                     /gene="gatC"
                     /locus_tag="AC16Y1_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR5832031.1"
                     /translation="MSGISKDDVAHLGRLARIDIPEDQLEHYAKQLDAILDAVAQVSE
                     VAAKDVPAMSHPVPMTNVFREDLPSESLANGRAVAGAPAVEDGRFRVPRILDED"
     gene            1323..2828
                     /gene="gatA"
                     /locus_tag="AC16Y1_05935"
     CDS             1323..2828
                     /gene="gatA"
                     /locus_tag="AC16Y1_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020644189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR5832032.1"
                     /translation="MSELIHLTAAQLAEKISKREVSSVEVTQAHLTRIEQVDQQVHAF
                     LYVNQEGALAAAKKADEDIAQGKPASVLAGVPLGLKDVLASEGLPTTAGSKILEGWIP
                     PYDATLVAKIRQAGIPILGKTNMDEFAMGSSTEHSAYGPTHNPWDLTRIPGGSGGGSS
                     ACLAAFEAPMSIGSDTGGSIRQPAAVTGTVGVKPTYGGVSRYGLIALASSLDQAGPCA
                     RTVLDTALLHSVIAGYDPKDSTSINKPVPAVVEAAKRGDIKGMKVGVIKELGGDGYQP
                     GVLARFNETVKIIEGLGAQVVEVSCPSFPYALAAYYLILPSECSSNLAKFDGIRFGLR
                     VGDDGSNSVEQVMSKTRAAGFGDEVIRRVILGTYALSAGYYDAYYGQAQKVRTLVAQD
                     FAAAFEKVDVLISPTAPTTAFKIGEKLDDPLAMYLNDIATIPVNLAGLPGMSIPIGLA
                     PEDNLPVGLQIIAPAMADERLYNVGAAIERALTQTWGGHLIEKIPALAVNK"
     gene            2840..4339
                     /gene="gatB"
                     /locus_tag="AC16Y1_05940"
     CDS             2840..4339
                     /gene="gatB"
                     /locus_tag="AC16Y1_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004983146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR5832033.1"
                     /translation="MKFDEAISKFDPVLGLEVHVELGTKTKMFCGCSTQFGAKPNTQV
                     CPVCLGLPGALPAVNKIGVESAIRIGLALNCEIATWCRFARKNYFYPDMPKNFQTSQY
                     DEPIAFNGFLDVDVDTDEGRKTFRIEIERAHMEEDTGKNSHVGGATGRIHGADYSLVD
                     YNRAGIPLIEIVTKPIENTGKYAPEVAKAYVSALRDILRALDVSDVKMEQGSLRCDVN
                     LSLRESAASPLGTRSETKNVNSLRSVERAVRYEVTRQAARLTAGEKVVQETRHWHEDT
                     GVTTSGRSKEQAEDYRYFPEPDLLPIAPSREWVEELRQTLPENPAMRKSRLMSEWGIS
                     ELEMQTVINAGALDLILAAINAGVPQSAARKWWTGELARIANDQGVELETLSITSAHL
                     LRIEELIADGSLNDKLARQVIEALLGGETDVDAVIANRGLAVVSDDGPLIAAIDDALA
                     ADPEVAAKIQSGKLQAAGAVVGAVMKATRGQADAARVRELLFERLGVSE"
     gene            complement(4340..5776)
                     /locus_tag="AC16Y1_05945"
     CDS             complement(4340..5776)
                     /locus_tag="AC16Y1_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR5832034.1"
                     /translation="MSLTPGPAEDLGFSSISDLRADLDENLISPRELVAELTDRINQI
                     DDCENGLNSVLAINSTLETQLEKVDLTKPLGGIPILIKDNIESIGLPATAGSLALVGR
                     PVIKSAPLVNTLENSGAIILGSTNLSEWANIRSGNSTSGWSAVGGLTANPWKYSHSAG
                     GSSSGSGAAVAAGLVPVAIGTETDGSIVCPASLNGIVGIKPTVGSVSTQGIIPISSSQ
                     DAPGPLARNVFDAATVLEVLMNKKGLVEQVSNPISLTIGVVRKWLTTDQATNDIFENQ
                     VSELAKSGIKIVEVEVPDISDEVGSDELTVLLCELVHTMDSYLASRAGDGVKSLAEVV
                     EFNRLNKERELAHFGQEFFEMAIKSGGLTDDYRLARNRNLTWAKSQVLDPAFANVDVL
                     VGIPYGPSWRSDLVNGDNFASASWMTNPAAISGYPIASIPMGFVADLPVGMGLIVSAG
                     NEVKLVNALSQIETVFDLADLIPAFNQN"
     gene            5821..6732
                     /locus_tag="AC16Y1_05950"
     CDS             5821..6732
                     /locus_tag="AC16Y1_05950"
                     /inference="COORDINATES: protein motif:HMM:NF014841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR5832035.1"
                     /translation="MSRHLIAPDWVISGYEFPADLKVTECDDLTILSDEALNSADFIV
                     IPYLQDTDQIEATLKRLTIPCVLQSLTAGYNHITPFLASGITLCNAVGVHDDSTSELA
                     VLLALSMLREIPRLVQGQEKQIWDQFFSKSLADKKVLLIGYGGVGKATEQRLLGFQCE
                     VIPVATKARNHVRAVSELPQLIPTADVIILTVPLNDSTYKLVDQSFISQMKYGSLLVN
                     VARGPVVDTEALLAALQAEKIFAALDVTDPEPLPEGHPLWQLSNCFITPHLGGESDVF
                     APRAQAMIRSQLDLWLSGRPLENVVST"
     gene            6758..8446
                     /gene="ilvD"
                     /locus_tag="AC16Y1_05955"
     CDS             6758..8446
                     /gene="ilvD"
                     /locus_tag="AC16Y1_05955"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR5832036.1"
                     /translation="MPDMKPRSKEVTEGLERAPARGMLRAVGMGDEDFAKPQIGIASS
                     WNEITPCNLSLDRLAKASKEGVFKAGGFPMQFGTISVSDGISMGHEGMHFSLVSREII
                     ADSVEAVMNAERLDGMVTFAGCDKSLPGMLMASARLDVAAVFVYAGSILPGNVTLSDG
                     SNRDVTIIDAFEAVGACARGLMSAEDVDRIERAICPGEGACGGMYTANTMASAAEAMG
                     MSLPGSAAPPSVDRRRDSYAIASGEAVVELIRQGITTRDIITKKSLENAIAVLMAFGG
                     STNAVLHLLAIAHEADIDLTLNDFKRIGAKVPLLADVKPFGRYVMSDVDKVGGVPVVM
                     RALFDAGLIHGDCLTVTGKTVAENLAQVDSPDPDGVILHAVKNPIGGVGGITILEGSF
                     APEGAVCKTAGIPVDVFEGPAKVFERERSAMDALEKGDIKAGDVVIIRYEGPKGGPGM
                     REMLMITGAIKGAGLGKEVLLITDGRFSGGTTGLCVGHVAPEASDGGPIAFVETGDRV
                     RIDIPNGKLDLLVDPAVLAERKKSWKVVPHKFNRGVLHKYAKLVGSASKGAVCD"
     gene            8439..>8789
                     /locus_tag="AC16Y1_05960"
     CDS             8439..>8789
                     /locus_tag="AC16Y1_05960"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5832037.1"
                     /translation="MTNLGINFPTQTVKAARWTIYFAFVYNGTSMGLFVSRLPDIRNY
                     FGLSNAQLGLVTFIGSLGIFFAMKPAGHWAAKYGSSRVLVHATWAYGLSTILLGLLLS
                     VPWLIGTLFLTGFLV"
BASE COUNT         2444 a         1809 c         2095 g         2441 t
ORIGIN      
        1 tatttttacg taaagctgga gatgtcattc cagaaatttt gggaccagtg gttgagctac
       61 gcgatggtaa cgaaaaagct tttaagatgc caaaaaaatg tccagaatgc aatagcccac
      121 tggctccaga aaaagaaagt gatgtggaca tcaggtgtcc gaatagccag tcctgtccag
      181 cacaactaag agggcgatta gaacatttgg gttcgcgttc cgttcttgat atcgaaggcc
      241 ttggtgaaaa agcagctcga gcgcttctag aagacaaaat aattaatgac gaaggcgatt
      301 tgtttgcttt aactgaagca aaactaaaaa agagtgattt ttttgttcgc ggcgcaaatc
      361 gtgaatttgc cgaaaatgga aaattactct tggcccaaat tgaacttgca aaaacaaaac
      421 cactttggcg catactttcc gcactctcaa ttcgtcatat tggaagtacc acggcccaag
      481 caatagcaaa tgagtattca tccattgatc aattagctaa agccaaagtt gaagagatcg
      541 cgcaagtcga aggaattgga caaactatcg cccaggcgat tgtggaatgg tttagcgaac
      601 cctggcatcg tgatgttgtt gagaagtggc gtaaagctgg cgtgcaactt gcgcaagaaa
      661 aaattgtcac ggggccacaa atacttgctg gcttaactct cgtggttacc ggaagtttag
      721 aaaagtactc tcgcgaaggt gctcaagagg caattacctc tagaggtgga aagtgcgcaa
      781 gttcggtttc aaaaaatact gactttgtgg tgattggtcc cggtgctggc tcgaaagcga
      841 aaaaagccga agaacttggt cgcccgattt tagacgaggc tggctttgat cttttattaa
      901 ccagtggacc cgaagcggtt aaagctaagt atttttcata actttgcttg tttggtatta
      961 acggaaattg caaattttta ctttgcgaat tcaccaatag aattgctcac atgtctggaa
     1021 tttctaaaga tgatgtagcc cacttaggtc gcttagccag aatagatatt ccagaagacc
     1081 agcttgaaca ttatgcaaag caattagatg caatcttgga cgcagttgcc caagtttcag
     1141 aagttgccgc taaagatgta ccagcgatga gtcatccagt gcccatgaca aatgttttcc
     1201 gtgaagattt accgagtgag tcactggcaa atggtcgggc agtggccgga gctccggcag
     1261 tggaagatgg tcgatttaga gtgcctcgta ttttggatga agattaataa tgaggcttat
     1321 aaatgtctga attaatccac ttaaccgcgg cacaattagc cgaaaaaatt tcgaaacgtg
     1381 aagtttcttc agttgaagtc acgcaggctc acttaaccag aattgaacaa gtagaccaac
     1441 aagttcacgc atttctttat gtaaatcaag aaggtgcgct tgccgcagca aaaaaagctg
     1501 atgaagatat cgcccaagga aaaccagcat ctgtacttgc tggtgtgcca cttggcctga
     1561 aagacgtttt agcaagtgaa ggtttaccaa ctactgctgg ctcgaaaata cttgaaggct
     1621 ggatcccgcc ctacgacgca accttggtag caaaaattcg ccaagcaggt attccaattt
     1681 tgggtaaaac aaatatggat gagttcgcaa tgggctcatc tacagagcat tctgcgtatg
     1741 gtccaacgca taacccttgg gatctaacgc gaattccagg tggttctggt ggcggatctt
     1801 ctgcgtgctt agctgctttc gaagcaccaa tgtctatcgg tagtgacact ggtggttcta
     1861 ttcgtcagcc tgcagcagta acaggaacag ttggagttaa accaacctat ggcggcgtta
     1921 gccgttatgg cttaattgcg ctagcttcat cccttgatca agctggccca tgtgcgcgaa
     1981 cagttttgga tactgcatta cttcacagtg tcattgctgg atatgaccct aaagattcca
     2041 cttcgatcaa caagcctgta ccggcagttg tcgaagccgc aaaacgcggc gacattaaag
     2101 gcatgaaagt cggtgtcatc aaagaattag gtggcgatgg ttatcaacct ggggtgctgg
     2161 ctcggtttaa cgaaacggta aagataattg agggacttgg tgcgcaagtt gtcgaagtta
     2221 gttgcccgag tttcccttat gcacttgctg cttattactt aatcttacca agtgagtgct
     2281 caagtaattt agcaaaattt gatggaatta gatttggatt acgcgtcggt gatgatggca
     2341 gtaactctgt agaacaggtt atgagtaaaa ctcgtgctgc tggtttcggt gatgaagtta
     2401 tcaggcgcgt aatactcggt acctacgcac tttctgctgg ttactacgat gcttattatg
     2461 gccaagcgca aaaagtaaga actttagttg cgcaagattt tgctgcagca ttcgaaaaag
     2521 ttgacgtttt gatttcaccg actgcaccaa ccaccgcatt caaaattggc gaaaaattgg
     2581 acgatccact tgctatgtat ttaaatgaca tcgccacaat cccagttaac ttagctggac
     2641 taccgggtat gtcaataccg attggtttag cgcccgagga taatctgccg gttgggttac
     2701 agattattgc tccagcaatg gcggatgaac gtttatataa cgtcggcgcg gcaattgagc
     2761 gcgcgctaac ccaaacttgg ggcggacatt taattgagaa aattccagcg ttggcggtga
     2821 acaagtgagc gagccaacaa tgaaatttga tgaagcaatt tctaaatttg atccagtact
     2881 tgggttagag gttcacgtcg aactgggtac taaaacaaaa atgttttgtg ggtgctcaac
     2941 gcaattcggg gcaaagccaa atactcaagt ttgtcctgtt tgcttgggtc ttccaggtgc
     3001 acttccggct gtaaacaaaa ttggtgttga gtctgcaatt cgaattgggc tggcccttaa
     3061 ttgtgagatc gctacttggt gtcgttttgc tcgcaagaac tatttttatc cagacatgcc
     3121 aaagaatttc caaacctctc aatatgacga accgattgcg tttaatggct ttttagatgt
     3181 tgacgtagat actgatgaag gacgtaaaac atttcgaatt gaaattgaac gcgcccatat
     3241 ggaagaagat acgggtaaaa attcacacgt tggtggtgct actgggcgca tccatggcgc
     3301 agattattcg cttgttgatt acaaccgtgc tggtattcca ttaatcgaaa tagtcaccaa
     3361 gccaatagaa aacactggca agtatgcccc tgaagtagcg aaagcttatg taagcgccct
     3421 gcgagatatt ttgcgtgccc ttgatgtatc agatgtaaaa atggagcagg gttctttacg
     3481 ctgcgatgta aatttatctc tgcgcgaaag tgctgcatct cctttaggca ccagatctga
     3541 aaccaaaaat gtcaattcac ttagatcagt agaacgtgca gttcgctacg aagtcaccag
     3601 acaagctgct cgcttaactg caggagaaaa agtagttcaa gagactaggc actggcatga
     3661 agacacgggt gttacaactt caggtcgttc taaagaacag gctgaagatt atcgatattt
     3721 tcctgaacct gacttactgc cgatagcgcc ttcgcgcgaa tgggtcgaag aattacgcca
     3781 aacgcttcca gaaaatccag ctatgcgtaa atctcgcttg atgagcgagt ggggtatttc
     3841 agaacttgaa atgcaaacag tcataaatgc tggagcactt gatttaattt tggccgcaat
     3901 taatgccgga gttcctcaaa gcgcggcccg taaatggtgg actggtgagt tagccagaat
     3961 tgccaacgat caaggcgttg agttagagac gctttcaatt acttcagcac atctattgcg
     4021 catcgaagag cttattgccg atggttcgtt gaatgacaaa ctagctcgtc aagttattga
     4081 agcacttctt ggtggtgaaa ctgacgtgga tgcagtaatt gcaaatcgtg gtctggccgt
     4141 ggtttctgat gacggcccac tgattgccgc aattgatgat gcattggcag ccgatccaga
     4201 agtagcagca aaaattcaat cgggtaagtt gcaagctgca ggtgcagtag tcggcgccgt
     4261 aatgaaagca acacgtggtc aagctgatgc tgctcgcgtt cgcgagttac ttttcgagcg
     4321 tttaggcgta tctgagtagt taattctgat taaatgctgg aattaaatca gccaagtcga
     4381 atactgtctc aatctgactt agtgcattga ccagttttac ttcatttcca gccgacacaa
     4441 ttagacccat gccaactggc aagtccgcaa caaatcccat tgggattgaa gcgattggat
     4501 atcctgaaat agcagctggg ttggtcatcc aactagcgct cgcaaaatta tcgccattaa
     4561 ctaagtcact acgccaactt ggtccgtaag gtatgccaac tagcacgtca acatttgcaa
     4621 aagctggatc tagaacctga gatttagccc aggttagatt tctatttcga gctaatcggt
     4681 aatcgtcagt taagccacca gatttaatcg ccatttcaaa aaactcttga ccgaaatgag
     4741 ctagctccct ttctttatta agtcgattga attccactac ctctgccaat gacttaacgc
     4801 catcaccagc acgactggct aagtaactat ccattgtgtg tactaattcg caaagcagaa
     4861 ctgtcaattc atctgagcca acttcatcag aaatatctgg tacttcaact tcaacaattt
     4921 tgataccaga tttagctagt tcacttactt ggttttcaaa gatatcgttt gtggcttgat
     4981 cagtcgttaa ccatttgcgg acaacaccga ttgttaagga aattggattg gatacttgtt
     5041 caacaagtcc ttttttgttc atcaagactt ctagcacagt tgcggcatca aaaacatttc
     5101 tagcaagtgg tcctggcgca tcttgacttg aactaattgg aattatgcct tgtgttgaaa
     5161 cactgccgac agttggcttg attccgacaa ttccgttcaa agacgctgga caaacaattg
     5221 acccatctgt ttcggtgccg atagcaaccg gaactaatcc agcggcgact gcagctccag
     5281 aaccagaaga acttccgcca gcactatggg aatatttcca tggatttgca gttaacccac
     5341 caactgcaga ccaaccacta gtggaatttc cagaccgtat atttgcccat tcggacaagt
     5401 tagtactgcc caaaataatt gccccagaat tttctaaagt gttaacaagt ggggcacttt
     5461 ttatgaccgg tctaccaact aaggccagtg aaccagctgt ggctggcaaa ccaatagatt
     5521 caatgttgtc tttaatcaga atcggtatgc caccaagtgg tttggttagg tctacttttt
     5581 caagttgagt ttcaagcgtt gagtttattg ctagaaccga attcaagcca ttttcacaat
     5641 catcaatttg attaattcga tccgttaact cagctactaa ttcacgggga ctaattaggt
     5701 tttcgtctag atcagctcgt aagtctgaaa tagaactaaa ccctaggtct tcagcggggc
     5761 cgggcgtaag cgacataggt catatagttc cacagaactt gggcgggcag aatagggagg
     5821 gtgagccgac atttaatcgc accggattgg gttatttctg gttatgaatt tccggcagat
     5881 ctaaaagtta ctgaatgtga tgacttaact attcttagcg atgaagcgct aaatagcgca
     5941 gacttcattg tgattccata tttacaagac accgaccaaa ttgaagccac cctgaaacgc
     6001 ttaacaattc cttgtgtact gcaatcttta actgcagggt acaaccacat caccccattc
     6061 ttggcaagcg gaattacgtt gtgcaatgca gttggtgtac atgacgacag cacttcagaa
     6121 ttagccgtgc ttttagcgct aagtatgctg cgtgaaatac cacgacttgt tcaaggtcaa
     6181 gagaaacaaa tttgggatca attttttagt aagtcacttg ctgataaaaa agttttactt
     6241 attggatatg gtggcgttgg aaaggctact gagcaacggt tattaggttt ccagtgtgaa
     6301 gtaattccag tggcaaccaa agcacgaaat catgtgcggg cggtcagtga gttacctcag
     6361 ctaatcccaa ctgcagacgt aattattttg acggttccgt taaatgattc aacctacaaa
     6421 cttgttgatc aaagttttat tagtcaaatg aaatatggtt ctttgttagt aaatgttgct
     6481 cggggacctg tagtggatac tgaagcttta ttagcggcgc tgcaggctga aaagattttt
     6541 gcggcgctag acgtcacaga tcctgagccc cttcccgaag gacatccact ttggcagcta
     6601 tcaaattgct ttataacgcc tcatctaggt ggcgaatctg atgtctttgc accaagagcg
     6661 caagcaatga ttcgaagcca attagatttg tggttatcgg gtcgaccact tgaaaatgtg
     6721 gtttcaacct aaattatttg aagggtagat ttggtttatg ccagatatga agccacgcag
     6781 taaagaagtc accgaaggac tagaacgcgc cccagctcgc ggtatgttgc gagccgttgg
     6841 aatgggtgat gaagattttg caaagccgca aattggtatt gcttcatcat ggaatgaaat
     6901 cacgccttgt aatttgtcac ttgatcgttt agcaaaggct tctaaagaag gtgtgtttaa
     6961 agctggtggt ttcccgatgc agttcggaac gatatcagtt tctgatggta tttcaatggg
     7021 tcacgaaggt atgcattttt cattagtgag ccgcgaaatt attgctgact cagttgaagc
     7081 agtaatgaat gcggaacgcc tggatggaat ggttacattc gctggttgcg ataagtcact
     7141 tccaggcatg ttaatggctt cggccagact cgacgtagct gctgtttttg tttatgccgg
     7201 atcgattttg ccaggtaatg tgacgctttc agatggaagt aatcgagatg taacgatcat
     7261 tgatgctttc gaagctgttg gtgcttgtgc ccgcggctta atgagcgcag aagatgttga
     7321 tcgcattgag cgagcaattt gtccaggtga aggcgcctgt ggcgggatgt acaccgcaaa
     7381 cacaatggct tctgcagcag aggctatggg tatgtcacta cctggttcag ctgcgccacc
     7441 ttcagtggat cgacgtcgcg atagctatgc aattgcttca ggtgaagcag tggtagaact
     7501 aattcgccaa ggcataacta ctcgcgacat cattactaaa aagtcacttg agaatgccat
     7561 tgcagtatta atggcatttg gtggttcaac taatgctgtt ttgcatctac tggcaatcgc
     7621 gcatgaagca gatatcgatt taacgcttaa tgatttcaaa cgaattggtg caaaagttcc
     7681 attgttagcc gatgttaaac catttggacg ttatgtaatg agtgatgtcg acaaagtcgg
     7741 tggtgttcca gttgttatgc gcgcactatt tgatgctggt ttgatccacg gtgattgctt
     7801 aactgtaacc ggtaagactg ttgccgaaaa tctcgctcaa gtagattcac ctgatcctga
     7861 tggggtaatt ttgcacgcag ttaaaaaccc aattggtgga gttggtggaa tcacaattct
     7921 cgagggttca tttgccccag aaggcgcagt atgtaaaacc gctggcattc cagttgatgt
     7981 tttcgaaggt ccagcaaaag tttttgaacg tgaacgtagc gctatggatg ccttagaaaa
     8041 gggtgacatt aaagctggtg acgtagtgat tattcggtac gaaggtccaa agggtggtcc
     8101 cggtatgcgc gaaatgttaa tgattaccgg cgcaatcaaa ggtgccggtt taggcaaaga
     8161 ggttttgcta atcactgacg ggcgctttag tggtggcaca actggactat gtgtcggaca
     8221 cgttgcccct gaagcaagtg atggtggccc aatcgctttt gtcgaaactg gcgatcgagt
     8281 tcgaattgat attccaaatg gcaaactcga tttattagtt gatccagcag tacttgctga
     8341 gcgtaaaaag agttggaaag ttgtgccgca taaattcaat cggggagttt tgcacaaata
     8401 cgcgaaatta gttggctcgg cttccaaggg tgcagtttgt gactaatttg ggaataaatt
     8461 ttccaaccca aactgtaaaa gcggctagat ggactatcta ctttgcattt gtctacaacg
     8521 gaacaagtat ggggcttttt gtctcacggc taccagacat tcgtaactac tttggattat
     8581 caaatgcaca actaggactt gtaactttta tcggatcctt gggcatattc tttgccatga
     8641 aaccagctgg gcattgggca gcaaaatatg gaagttcgcg agttttggtg catgccactt
     8701 gggcgtatgg cctatctaca attttgctcg gcttattact tagtgtgccg tggttaattg
     8761 gtaccctctt tcttactggc tttttagtc
//
LOCUS       DBNHVN010000311         1837 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1323000, whole genome shotgun sequence.
ACCESSION   DBNHVN010000311 DBNHVN010000000
VERSION     DBNHVN010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1323000"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="AC16Y1_05965"
     CDS             complement(<1..242)
                     /locus_tag="AC16Y1_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5832038.1"
                     /translation="MTNQVNVNRIRLILRAGSIVFGFSALTLLALPSFFNELLGFVPS
                     SELDWAMRMIGITLVALAGNMFSVSTRGTVESVLFSG"
     gene            complement(266..1213)
                     /locus_tag="AC16Y1_05970"
     CDS             complement(266..1213)
                     /locus_tag="AC16Y1_05970"
                     /inference="COORDINATES: protein motif:HMM:NF046806.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5832039.1"
                     /translation="MINWGFLGASWVAGKALVPAIKTAPNANLYAVASRDQKRSATFE
                     PTKVHATYADLITDPNVHAVYISLANHLHLDWTIKALNAGKHVLCEKPLAITSDDAKL
                     MAQAATANDRLLVEAIWTRWHPRFVRMVELVKAGEIGQLKSIDSTFTFTREVDDNYRM
                     NPQQGGGSLLDVGPYQVHVWAALAGASTSFSITSATQNIGPTGVDLTTEVQALLADQI
                     KVSAVTSFEQAAVQHLLISGDTGTLEFPTGQAFTSWNEESSLRIGNYEEKFAPVDPFA
                     LMVTDFGNHLAGNDSWLPRIEESIFVASIMDNVKSYCQS"
     gene            complement(1210..>1837)
                     /locus_tag="AC16Y1_05975"
     CDS             complement(1210..>1837)
                     /locus_tag="AC16Y1_05975"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5832040.1"
                     /translation="TMKHLIESGEIGEVFHYESFVGGYSKPCSYWHSDADVSGGAIFD
                     WGSHFIDQILDLVPAKVDFVSGLNQKRVWDHVTNADHAQVTINFKDGVLATFINSDLA
                     AARKPKFYVLGTKGAIIGNWDPAAGDSVANLPALISVHREDGTIKNFPHIEVEPFSFH
                     TSLVEYLNNRKPMEVVAEQSRDVVAIMQAAEESALKNGVPVTPTLLRS"
BASE COUNT          532 a          402 c          398 g          505 t
ORIGIN      
        1 ccagagaata aaactgattc aacggtgcca cgagttgaca cagaaaacat atttccagcc
       61 aaggcgacta aagtgatccc gatcattcgc atagcccaat caagttccga acttggtacg
      121 aaacctaaaa gttcgttgaa gaatgaaggt agtgcaagaa gtgtaagtgc actaaaacca
      181 aaaactattg aaccagcacg aagaattagg cgaatacggt tcacgttaac ttgatttgtc
      241 aagttctatt cctttactaa attaactaag actggcaata cgatttaaca ttatccatga
      301 tcgaagcaac aaaaatcgat tcttcgattc ttggcagcca actatcgttg ccagccaaat
      361 gatttccaaa gtcggtcacc atcaaggcaa atggatcaac tggagcaaat ttttcttcat
      421 agttgccaat tcgaagcgaa ctttcttcat tccaagaagt gaaagcttga ccggttggaa
      481 attcaagggt cccagtatca ccagaaatta gcagatgctg caccgcggct tgctcaaagg
      541 aagtgaccgc tgaaactttt atctgatcag ctaaaagtgc ctgtacttct gtagttagat
      601 caactccagt cggtccaata ttttgagtcg ccgaagtgat tgaaaaactg gttgaagcac
      661 cagcaagtgc tgcccaaaca tgaacttggt acggcccgac atctaataaa ctgccgccac
      721 cctgctgggg attcatgcga tagttatcgt caacctctcg ggtaaacgta aatgttgaat
      781 caattgattt aagttggcct atttcacctg ccttaacaag ttcaaccatg cgcacaaaac
      841 ggggatgcca tctagtccag atagcctcaa ctaaaagccg atcatttgct gtggctgctt
      901 gtgccataag ttttgcgtcg tcacttgtga tggctagcgg tttttcacat aaaacatgtt
      961 tgccagcatt cagcgctttt attgtccagt caagatgtaa atgattggca agagagatat
     1021 aaaccgcgtg gacgtttggg tcagtaatta aatctgcgta ggtggcgtgt acttttgttg
     1081 gttcaaatgt cgcagatctt ttttgatcac gactagcaac tgcatagaga tttgcattag
     1141 gtgcagtttt tatagccggc actaatgctt tgccggcaac ccagctagca ccgagaaatc
     1201 cccaattaat catgaacgca aaagtgtggg agtcaccgga acgccatttt taagtgctga
     1261 ttcttcggcg gcttgcatga tggctactac atcgcgagat tgttcagcaa cgacttccat
     1321 tggttttcga ttgtttaaat attccactag tgaagtgtgg aatgaaaatg gctcaacttc
     1381 aatgtgtggg aagtttttaa ttgtgccatc ttctcggtgt accgaaataa gtgctggtaa
     1441 atttgcaaca gaatcaccag ctgctggatc ccaattgccg attattgcgc ctttagtccc
     1501 aagcacataa aacttaggct tacgggcagc agctaggtct gagtttataa aggtagccag
     1561 aactccgtct ttaaagttaa ttgtcacttg agcatgatct gcattcgtga catggtccca
     1621 gacacgtttt tgatttagcc cacttacgaa gtcaactttg gctggaacta gatccaggat
     1681 ttgatcgatg aagtgactgc cccagtcaaa gatcgcgcca ccggaaacat cagcgtcaga
     1741 gtgccagtat gaacagggtt tggagtagcc accgacaaaa ctttcatagt ggaatacctc
     1801 acctatttca cctgattcaa ttaagtgttt catggtc
//
LOCUS       DBNHVN010000312         1621 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_133314, whole genome shotgun sequence.
ACCESSION   DBNHVN010000312 DBNHVN010000000
VERSION     DBNHVN010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_133314"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1399)
                     /gene="lpdA"
                     /locus_tag="AC16Y1_05980"
     CDS             complement(41..1399)
                     /gene="lpdA"
                     /locus_tag="AC16Y1_05980"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR5832041.1"
                     /translation="MHDVVILGGGSGGYACALRSAQLGLSVVLIEQDKLGGTCLHRGC
                     IPTKALLHAAEVADTAREGENYGVHSTFNGIDMNKVNSYKDGVIGRLYKGLQGLVKSR
                     EITYVEGFGKLVAKNAVEVNGQRYEGKNIVLATGSYAKSLPGLEIAGRIMTSDQALTM
                     DYVPTSAIILGGGVIGVEFASVWKSFGVDVTVIEGLPRLVPAEDEAISKTMERAFRKR
                     GINFKTGVKFQSAATDDSGVTVTLENGETVRADLLLVAVGRGPRTEGLGYEDQGVNMD
                     RGFVLANDRLATNVPGIHAVGDIVPGLQLAHRGFQQGIFVAEEIAGLNPKAIDETGIP
                     RVTYSEPEIASIGYTESQAKEKFGEIDSYEYNLGGNGKSQILSTAGFIKLIRKKDGPV
                     VGVHMIGSRVGELVGEAQLIYNWEATADDVAPLVHAHPTQNEALGEAFLALAGKPLHA
                     HA"
BASE COUNT          429 a          417 c          326 g          449 t
ORIGIN      
        1 attctcctta ttaatctgtc ttaataattt tagttaactt ttaggcgtgg gcgtgcagcg
       61 gttttccagc caatgccaag aaagcctcac caagtgcctc attctgggtc ggatgagcat
      121 gaactaaagg cgccacatca tcggcagtag cttcccagtt gtaaatcaat tgagcttcac
      181 caacaagttc accaacgcgt gaaccaatca tgtgtacacc aaccactggg ccatcttttt
      241 tgcggattaa tttaatgaat ccagcagtcg atagaatctg gctctttcca tttccaccta
      301 agttgtattc gtatgaatca atttcgccaa atttttcttt cgcttgcgac tcggtgtagc
      361 cgatagatgc tatttcaggt tcactatagg taacccgagg aatgccggtt tcatcaattg
      421 cttttggatt aagaccagca atttcttcag ccacaaaaat accttgctgg aatccacggt
      481 gggccagctg tagaccagga acgatgtcac caaccgcgtg gattccaggc acatttgttg
      541 ctaatcgatc attggctaaa acaaagcccc gatccatgtt tacgccctgg tcttcataac
      601 ccaagccttc agtgcgaggt ccgcgaccga ccgcaactag caataaatct gcgcgaacag
      661 tttcaccatt ttctagagtc acagtcacgc cagaatcatc agttgcggca ctctggaatt
      721 taactccagt tttaaagtta atgccgcgtt tacggaaggc acgttccata gtttttgaaa
      781 ttgcttcgtc ttcagctgga actaagcgcg gtaagccttc aattacagtt acgtcaacgc
      841 cgaatgattt ccatacagat gcaaactcaa cgccaataac accgccaccc aaaataattg
      901 cacttgttgg cacgtagtcc atagttaggg cttggtcact tgtcattatt cgaccagcaa
      961 tttcaagtcc cggaagagac tttgcgtagg agccagttgc taaaacaata tttttgcctt
     1021 cgtaccgctg tccgttaact tcaaccgcat tcttggcaac aagttttcca aaaccttcaa
     1081 cataagttat ttcacggctt ttaactagac cttgtaaacc tttgtaaagg cgaccgatta
     1141 caccatcttt atatgaattg actttattca tgtcaattcc attgaaagtg ctgtgcacgc
     1201 cgtagttttc gccttcacgc gcggtatcag ctacctcggc tgcatgcagt agtgccttag
     1261 ttggaataca accccggtgt aagcaagtgc caccaagttt gtcttgctca attagaacta
     1321 cagataaacc aagttgtgct gaccggagcg cacatgcgta tccaccacta ccgccaccca
     1381 aaatcactac gtcgtgcatt aatcctccga aaacaatttc tggctgagta atacctagta
     1441 gctcttgcga cgatactcag tcgcttctat tgttccacta tttggaattg gcaccgacac
     1501 tggatatgca atcttgggca aaagcaacca tagttcgcac gccaaagcca acgccacctt
     1561 tatgcgtata gccatgggcc gcttgcgagt tgtaggccgg accggcaatg tctagatgtg
     1621 c
//
LOCUS       DBNHVN010000313         1335 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1770570, whole genome shotgun sequence.
ACCESSION   DBNHVN010000313 DBNHVN010000000
VERSION     DBNHVN010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1770570"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..941)
                     /gene="rsmA"
                     /locus_tag="AC16Y1_05985"
     CDS             complement(84..941)
                     /gene="rsmA"
                     /locus_tag="AC16Y1_05985"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR5832042.1"
                     /translation="MSELVLLGAAQIRELAAQLDIKPTKKLGQNFVIDPNTVARIANE
                     AKLNADDVVLEVGPGLGSLTLAMLPKVKKVLAIEVDQRLAQQLPLTVKKLTPDLVNRL
                     EVINEDALRITKLPESPTKLVANLPYNISVPVVIHLLETFESITEVLVLVQLEVAQRM
                     AATPGSKIYGVPSVKIAWFGDAALAGQVGRNVFWPAPNVDSGLVRITRAKERGSIELR
                     KKLFQVVDSAFGQRRKTLRSSLSSVFGSSTQVDQALAKAKVDSSLRGEQLGLADFLAI
                     AKVAGELND"
     gene            complement(954..>1335)
                     /locus_tag="AC16Y1_05990"
     CDS             complement(954..>1335)
                     /locus_tag="AC16Y1_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020385546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aggregation-promoting factor C-terminal-like
                     domain-containing protein"
                     /protein_id="HIR5832043.1"
                     /translation="VVVVGTGDPETIRNAEIAQGINIQSIADAKKFTELFIAATYGWD
                     AQQFSCIENLWEKESNWRVTADNPTSSAYGIPQALPGSRMAEIADDWRTNPVTQIKWG
                     AQYIEKRYSTPCVAYNKALNVGWY"
BASE COUNT          352 a          323 c          261 g          399 t
ORIGIN      
        1 attctcttgg tccaacacct agatacagat tcactttgcc tgggacccga acgacaactt
       61 gagcaggaag taatgctgat gcactagtca tttaattcac ctgccacttt cgcaatcgct
      121 agaaaatcag ctagcccgag ttgttctccg cgtagcgaac tatctacttt tgctttagct
      181 aaagcctgat cgacctgagt cgaacttcca aatactgatg acagcgatga cctaagggtt
      241 tttctacgtt ggccaaatgc actgtcaact acttgaaaga gctttttgcg taattcaata
      301 cttccacgtt cttttgcccg cgtaatccga actaaaccag aatccacatt tggtgctggc
      361 caaaatacat ttcgacctac ttgtccagcc agtgcagcat caccaaacca agcaatcttc
      421 acactcggca caccatagat tttgctacct ggagttgctg ccatgcgctg cgcaacctca
      481 agttgcacca gcactaaaac ttcagtgatt gattcaaaag tttcaagtaa gtgaatgact
      541 accggaactg aaatgttgta cggcagattt gccactaatt tagttggcga ctctggcaat
      601 ttagtaatcc gaagtgcatc ttcattgatt acttccagtc gattgaccaa atctggcgtt
      661 aattttttaa cggtcagcgg taactgctgg gcaaggcgtt ggtcaacttc gattgccaaa
      721 acttttttaa cttttggcag cattgccaaa gtaagtgaac caagtccggg accaacttct
      781 agaaccacat catcagcgtt taactttgct tcattggcga ttctggcaac tgtgtttgga
      841 tcaattacaa aattctgacc aagttttttt gttggcttga tatccagttg cgcagctagt
      901 tcgcgaattt gtgctgcgcc tagcaaaact aattcgctca tgcactaagc ggcttaatac
      961 cagccgacat ttaatgcttt gttgtatgca acgcaaggag tgctgtaacg cttttcaatg
     1021 tattgcgcac cccacttaat ttgcgtaact ggatttgttc tccagtcgtc tgcaatttcc
     1081 gccatccgcg atccaggaag tgcttgtgga attccgtatg cacttgatgt cggattatca
     1141 gcagtaactc gccaattact ttctttttcc caaagatttt ctatacaact aaattgctga
     1201 gcatcccaac cataagttgc ggcaatgaaa agttctgtga atttctttgc atcggcaatt
     1261 gattgaatgt ttatcccctg cgcaatttca gcattacgaa tagtttctgg atcgcctgtg
     1321 ccaacgacaa caact
//
LOCUS       DBNHVN010000314         1334 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1770636, whole genome shotgun sequence.
ACCESSION   DBNHVN010000314 DBNHVN010000000
VERSION     DBNHVN010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1770636"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="AC16Y1_05995"
     CDS             complement(<1..487)
                     /locus_tag="AC16Y1_05995"
                     /inference="COORDINATES: protein motif:HMM:NF017200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HIR5832044.1"
                     /translation="MGLARARSVVLTGVQGMLVEIEAYISAGLPSMNIVGLGDTAVAE
                     ARDRVRAAVSNSKLSWPSERKITVGLSPASQHKKGSGLDLGIALAILAANGEVPEVTD
                     FIVVGELALDGKVRPTPGVLVAALTAYQLGFTKIMVPINQLAEAQLVPDLAVIGVSCL
                     RD"
     gene            complement(478..843)
                     /locus_tag="AC16Y1_06000"
     CDS             complement(478..843)
                     /locus_tag="AC16Y1_06000"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR5832045.1"
                     /translation="MRLSNKELGKLGEDFATNWFTYRGFRLLDRNWRFGRVELDLVVS
                     KDGIVIFVEVKTRTNMEFGGAVHAISKQKLNNLNRAALHWLSQNRFDADGIRIDLMAL
                     QYDGENFRVIHIPGIGSWA"
     gene            complement(924..1232)
                     /locus_tag="AC16Y1_06005"
     CDS             complement(924..1232)
                     /locus_tag="AC16Y1_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008730273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HIR5832046.1"
                     /translation="MSADELERYEAELELELYREYRDLVHLFNYIVHTERRVYLANGV
                     DVQVRTNEAGDVYFEVVLSDAWIWDIYGQARFAKKVKVLTFKDVNVEELAKPDLDSLT
                     "
BASE COUNT          395 a          324 c          227 g          388 t
ORIGIN      
        1 catcacgtaa acaactcacc ccaatcaccg ctaagtcagg cacaagttgc gcttctgcca
       61 gttgattaat tggaaccata atttttgtaa atcccaattg ataagccgtt aaggcagcaa
      121 ctaaaactcc cggagttggt cgcactttgc catcaagagc taactcacca accacaataa
      181 agtcagtgac ttctggcact tcgccgttag ccgccaaaat tgctaaggca attccaagat
      241 ctaaaccgga acctttttta tgttgagatg caggtgataa tccaactgtt atttttcgct
      301 cacttggcca gctcaattta ctgtttgaaa cagcggcacg aacacgatca cgagcctctg
      361 caacggctgt gtctccaagt ccaactatgt tcatacttgg caatccggca cttatataag
      421 cttcaatctc aactagcatt ccttgcacac cggttaaaac cacacttcgc gcacgagcta
      481 agcccatgaa ccgatcccag gaatatgaat tactcgaaaa ttttcaccgt catactgcag
      541 cgccatcaaa tcaattcgaa ttccgtcggc atcaaatcga ttttgactta accaatgaag
      601 tgcagctcga tttaagttat ttaatttttg tttggaaatc gcatgaactg ccccaccaaa
      661 ttccatattt gttcgagttt ttacttcaac aaaaataact attccgtctt tactcactac
      721 taaatcgagc tcaacacggc cgaatcgcca atttctgtcg agtaagcgaa agcctctgta
      781 ggtaaaccaa tttgttgcaa aatcttcgcc gagtttgcct aattctttat ttgataagcg
      841 cacccaagaa tcatggatat atcaattgca aaataaaata agcagtttga tttgtggatg
      901 gattgaactt gtgatgaaac cagttaagtc agtgaatcta agtctggctt agccaattct
      961 tcaacattta cgtctttgaa cgttaaaact tttacttttt tggcaaacct ggcctgacca
     1021 taaatatccc aaatccaagc atcacttagt actacttcaa aataaacatc gccagcttca
     1081 tttgttcgaa cctgcacatc aacaccatta gctaaataaa ctcgccgctc agtgtgaact
     1141 atgtaattaa ataagtgaac tagatctcga tattcgcgat ataactcaag ttcaagttct
     1201 gcttcatatc tttctagttc gtcagctgac atagttctag cctattagtt ggcttttggc
     1261 agattccaag acctccggtg aaactgcgaa ggtccatgtt tttgaattgc cgccatgtgc
     1321 gctgcgctgc cata
//
LOCUS       DBNHVN010000315         1083 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_133621, whole genome shotgun sequence.
ACCESSION   DBNHVN010000315 DBNHVN010000000
VERSION     DBNHVN010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1083)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_133621"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..403
                     /locus_tag="AC16Y1_06010"
     CDS             179..403
                     /locus_tag="AC16Y1_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5832047.1"
                     /translation="MEKVNKPMIEFNGKMIEVDSEHDEDWLDDDSIPDEEKLAYFNSL
                     DWVPVRNLATEFGITSPEEHEEFLKKQAGG"
     gene            complement(400..>1083)
                     /locus_tag="AC16Y1_06015"
     CDS             complement(400..>1083)
                     /locus_tag="AC16Y1_06015"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HIR5832048.1"
                     /translation="VLEYCARNDESLTGNMMNKMSDALVGILTGQSEIAPERRHSAAQ
                     VQVVVSLETLLGLSETPADVIGSEFSITAGAIRDLAKDSHLRRMVVDPDSGALLELGR
                     KSYEPSRQIKDFVKLRDKKCRAPGCVRRAMACDVDHVKAWDDGGETNVDNLVSLCRRH
                     HVLKTIGTWQYELLPNGDTVWRLPSGHIVRDYASTWVEPAVNNVALDPEAIENAIRQL
                     NPDLEPAPF"
BASE COUNT          295 a          264 c          245 g          279 t
ORIGIN      
        1 ggtctattac ctgcaggcat ggctgctcct atttacttta aaagtgaccg ctatttaatt
       61 gtgatcctca gtctatatca aattcaagct acaagattta caatgcaaac ggacagatgc
      121 taactgccta tttgcatcca cttaaccaag aattgggcat ctggtttctt ggtacagcat
      181 ggaaaaagta aacaagccga tgattgaatt caacggcaaa atgattgaag tcgattccga
      241 acacgatgaa gactggttag acgacgattc aattccagac gaggaaaaac tcgcctattt
      301 caattcactt gattgggtcc cggttagaaa tctggcgact gaatttggaa ttacgtcacc
      361 agaggaacac gaagagtttc taaaaaagca ggctggcggc taaaacgggg cgggttccag
      421 gtcgggattc agttgccgga tagcgttttc gattgcttcc ggatcaagtg caacattatt
      481 caccgcaggc tcgacccacg tgctcgcgta atcccgaact atgtggccac tcggaagtcg
      541 ccaaaccgtg tcaccgttgg gtagcagctc gtactgccaa gttccaatgg ttttcaaaac
      601 atggtgtcgt cgacaaaggg ataccaagtt atcaacattc gtttcaccac cgtcatccca
      661 cgctttcaca tgatcgacat cgcaagccat agctcttcgc acacaaccgg gtgctcgaca
      721 ttttttatct cgcaacttca caaagtcttt gatttgccga ctaggttcgt aacttttacg
      781 gccaagttcg agtagcgctc cagaatctgg atcaacgacc attcggcgta ggtgtgaatc
      841 ctttgcaaga tcgcgaattg cgccagcagt gatcgagaat tcagacccaa ttacatcggc
      901 tggagtttca gatagcccaa gtaaagtctc aagactcacg acaacttgaa cttgagcggc
      961 actgtgtcga cgctctggag cgatttctga ttgaccggta agtatgccaa ctagcgcgtc
     1021 actcattttg ttcatcatgt tgccggtaag tgactcgtca ttgcgagcgc aatactccaa
     1081 aac
//
LOCUS       DBNHVN010000316         1085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_728426, whole genome shotgun sequence.
ACCESSION   DBNHVN010000316 DBNHVN010000000
VERSION     DBNHVN010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_728426"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="AC16Y1_06020"
     CDS             <1..741
                     /locus_tag="AC16Y1_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HIR5832049.1"
                     /translation="EARKTGAMALFGEKYGDEVRVISIGDWARELCGGTHTQTTGQLG
                     IVTFLSESSIGAGVRRVEALVGTDAYRFLAREHYLIDQLAQIVKVRSEELPDRINNLL
                     DRLKDAERQIAKAKTAALTEKLSELVGSPVNENGVSIYRFLAPTGVESSQLRDLVISA
                     KAQIKTTCNLIVGMVSEEGKISAVVTVDAGAKEKGLAANSVLNELMTHLAGRGGGNAD
                     LAQGGGTNLAGIDLAFDQVVESVRGAAR"
     gene            725..>1085
                     /gene="ruvX"
                     /locus_tag="AC16Y1_06025"
     CDS             725..>1085
                     /gene="ruvX"
                     /locus_tag="AC16Y1_06025"
                     /inference="COORDINATES: protein motif:HMM:NF015607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR5832050.1"
                     /translation="MALPGKGTRLAIDVGSVRIGIARCSTEQIMAVPECTVLASQDPI
                     KEIARLVDEYKIELVYVGNPISLAQKVTPSTELAKNFALELAKVLEVDVHMLDERLTT
                     VSAQTQLRDSGKSSKDGR"
BASE COUNT          319 a          213 c          281 g          272 t
ORIGIN      
        1 gaagctcgca aaactggggc gatggccctt tttggcgaga aatacggaga tgaagtaaga
       61 gtcatcagta ttggcgactg ggctcgagaa ctttgtggtg gaacgcatac gcagacgaca
      121 ggtcaattgg gtatagtgac gtttctttcc gaaagctcga ttggcgctgg cgtgcgcaga
      181 gttgaagccc tagtcggaac tgacgcatac cgatttcttg ctagagaaca ttacttgatt
      241 gatcaattag cacaaatagt gaaagtgcgc tccgaagagc ttccagatcg aattaacaat
      301 ctcctagatc gcttaaagga tgcagagcga caaatcgcta aagcaaaaac tgctgcacta
      361 actgaaaagc taagcgaatt agttggtagt ccagtaaacg aaaatggcgt gagcatttat
      421 cgattcttgg ctcctacagg agtcgaaagt tcacaacttc gagatctcgt tatttcagca
      481 aaggcacaaa ttaagacaac ctgtaatttg atcgtcggaa tggtttccga agaaggaaaa
      541 attagcgcag tcgttactgt agatgctggt gccaaagaaa aaggtttggc tgccaatagt
      601 gtcttaaatg aactaatgac tcatcttgct ggccgtggcg gtggtaatgc tgatttagcc
      661 caaggtggcg gaacgaattt agctggaatt gatctcgcct ttgatcaagt tgtagagagt
      721 gtacgtggcg ctgcccggta aaggaacacg actggccatt gacgtcggtt cagtacgcat
      781 cggtattgcc aggtgttcta ccgaacaaat tatggcggtt ccggagtgta cggttttggc
      841 tagccaagac ccgataaagg aaatcgctcg attggttgat gaatacaaga tagaacttgt
      901 ttatgtaggt aatccgattt cgctggctca aaaagttaca ccgtcaaccg aattggctaa
      961 aaatttcgca ctcgagttag caaaagtgct agaggttgat gtccacatgc tagatgaaag
     1021 actgaccacg gttagtgctc agacccaatt aagagattca ggtaaatcct caaaagatgg
     1081 acgtt
//
LOCUS       DBNHVN010000317         4366 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1323935, whole genome shotgun sequence.
ACCESSION   DBNHVN010000317 DBNHVN010000000
VERSION     DBNHVN010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1323935"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="AC16Y1_06030"
     CDS             <1..203
                     /locus_tag="AC16Y1_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5832051.1"
                     /translation="YDPNLPFAIYLPEDIETLRKSDLALVKMWRQEVRDLLVESFTNN
                     CQISQMVDDRKALLVTPMKGTE"
     gene            200..1309
                     /gene="menC"
                     /locus_tag="AC16Y1_06035"
     CDS             200..1309
                     /gene="menC"
                     /locus_tag="AC16Y1_06035"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020551880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HIR5832052.1"
                     /translation="MKLEAVELIRMDVPLKSAFRTSFSSVNLHQVVWAHVITDVGEGW
                     AECGANERPDYSSEFHNGAATVIKEFLLPQLFALGSDLTAESVAPTLEWVKGHRMAKA
                     VVETALLDAQLRSTNTSFATYLGAVKDKVPAGVSVGIMDSLPELMKFVEGYLADGYLR
                     IKLKIEPGWDYEPVKLVRETFGPDLLLQVDANTAYTREDFDLLKKLDEFNLLLIEQPM
                     NEEDMLGHAKLADYIETPVCLDESIVSADIARDAIELGAAEIINIKPSRVGGYLESKK
                     IHDVSAKAGIPVWCGGMLETGIGRAANLALAALPNFTLPGDTSASARYFETDVTEPFV
                     LVNGHVKVPTGPGIGVDPIPEIIDRFAVSREIVKR"
     gene            complement(1299..1757)
                     /locus_tag="AC16Y1_06040"
     CDS             complement(1299..1757)
                     /locus_tag="AC16Y1_06040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR5832053.1"
                     /translation="MDNHAKGRLLVAIQFLLLGGLILISSEDIFLGNNTIDAIGVAFE
                     LIGFVIIIFGFSGLGKSLTANPVPIKSGKLTTTGIYSRVRHPIYLGLLLVTLGITLTN
                     GSVVKFGFWLLLLSLLIFKIKFEETLLLMAYPGYKQYKEKVPAIFPKFTA"
     gene            1959..2360
                     /locus_tag="AC16Y1_06045"
     CDS             1959..2360
                     /locus_tag="AC16Y1_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-opening dioxygenase LigA"
                     /protein_id="HIR5832054.1"
                     /translation="MKNNKLIGLIAIVVGAIFIVAGGTTYNMVHNKLADEKITVSADA
                     ERYAGQQVAGPFTAYTEAMIIQKHALEATGGKTYAELDREDPLRAVAMNGSFLRASLF
                     TSVVSFGVAALAAGLGVMFVLLGVAIRNTAR"
     gene            2433..2615
                     /locus_tag="AC16Y1_06050"
     CDS             2433..2615
                     /locus_tag="AC16Y1_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5302 domain-containing protein"
                     /protein_id="HIR5832055.1"
                     /translation="MAKNESAKDPAKAAFLAALEKKKNSATKKAGESHAEGKGAAKGP
                     NSGVTRRIERRRSGSS"
     gene            2677..3759
                     /locus_tag="AC16Y1_06055"
     CDS             2677..3759
                     /locus_tag="AC16Y1_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011207642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_function: GO:0015369 - calcium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA];
                     GO_process: GO:0070588 - calcium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium:cation antiporter"
                     /protein_id="HIR5832056.1"
                     /translation="MVVKEIRSASNVIGPILAAIAVIYFWNNTPNALEVIFISIVLIG
                     GVTTAVHHAEIISAYVGRSLGALILALSVTIIEVGVIVAIMTSSDGAASTLGRDTVFA
                     AVMITCNGIIGFSVVAASLKQAAPTFNSEGSGAALSAIVTIATLSLVLPTFTRSSSGP
                     TLTTGQLTFVSIISLAVYALFLYVLTVRNREHFEDPEGQVVVPYAIHPTKRVFRQSVS
                     LLLISLTAIIGLAKVMSPSIESVVRDAGFPLSVVAVVVALVILAPEGIAAVKSSSRGD
                     LQTGFNLAYGSALASIGLTIPALAIASTLLGIELQLGLGATEIVLYVLTLLVSLVTVS
                     SHRTTFFQGGLHLVIFSAFIFLALSP"
     gene            complement(3765..>4366)
                     /locus_tag="AC16Y1_06060"
     CDS             complement(3765..>4366)
                     /locus_tag="AC16Y1_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013862453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase-like domain-containing protein"
                     /protein_id="HIR5832057.1"
                     /translation="NIFVVEDTPYRELRFAGTSLPTLKSMDTQGRVLHLGSFSKILSP
                     SMRLGWALASPEILEKLSLLKLAADTQNSTLNMNATLEYLRRYNIDEHLEKVRPVYRH
                     KRDLMAATMVEAFPSEVTFTKPEGGLFTWLTFPKEFDATKFMKEILIPQGKVAFVPGA
                     TFFPVEQESNHARFSFSGISDERLVKGISILGELLKQNL"
BASE COUNT         1211 a          906 c          966 g         1283 t
ORIGIN      
        1 cctacgatcc aaatttacct tttgcgattt atttgcccga agacatagaa actctgcgca
       61 agtccgacct ggctttagtc aaaatgtggc gtcaagaagt tcgagattta ctagtcgaat
      121 cttttaccaa taattgccaa ataagtcaga tggtcgatga tcgcaaagct ttactagtaa
      181 ccccaatgaa aggtaccgaa tgaaattaga agcagttgag ctaatccgaa tggatgttcc
      241 gctcaaatca gcatttcgta cttcatttag ttcagttaac ttgcaccaag ttgtctgggc
      301 acacgtcatc accgatgttg gtgaaggctg ggctgaatgc ggcgctaatg agcgtcctga
      361 ttactcaagt gaatttcata atggcgcggc aactgtaatt aaagagtttt tactacctca
      421 actattcgca ctgggctcag atttaactgc tgaaagtgtg gcaccaactc tggagtgggt
      481 taaaggccat cgcatggcca aggcagtggt agaaacagcg cttttggatg cgcaattacg
      541 ttctaccaat acgtcatttg ctacttactt aggcgccgta aaagataaag ttccagcagg
      601 cgtctcagtt ggaattatgg attcgctccc tgaattaatg aagtttgttg aaggctattt
      661 ggctgatgga tatttacgga tcaaactcaa aattgaaccg ggctgggatt atgaaccggt
      721 taaattagtt cgcgaaactt ttggccctga cttattactt caagtagacg ccaatactgc
      781 atatacccgg gaagactttg acttacttaa aaagttggac gaatttaatt tgctcttaat
      841 tgaacaacca atgaacgaag aagatatgct cggtcatgcc aaattggctg attacattga
      901 aactcccgtc tgcctggatg aatcaattgt ctcggccgac attgcaagag atgcaattga
      961 attgggagcc gctgagatta ttaacatcaa gccatctcgg gttggcggtt accttgaatc
     1021 taagaaaatt catgatgttt cggccaaggc tggaattcca gtttggtgtg gtggcatgtt
     1081 agaaactggt atcggtcgcg cagccaactt ggctttggct gcattaccta atttcacact
     1141 tcccggtgat acctcggcat cagcgcgtta ttttgaaaca gatgtcactg agccgtttgt
     1201 attagtcaat ggtcacgtca aggtaccaac cggtcctggt atcggcgttg acccgattcc
     1261 agaaatcata gatcggtttg ctgtgagccg cgaaatagtt aagcggtaaa tttaggaaaa
     1321 attgctggga ctttttcttt gtactgtttg taccctggat aagccatgag cagcaaggtt
     1381 tcttcaaact taattttaaa aattagtagt gatagcagta gtagccagaa accaaattta
     1441 acgactgatc cattagtcaa agttatgccg agagtaacta gtaataaccc caagtagatt
     1501 ggatgtctaa ctctcgaata aatgccggtg gtagtcaatt ttccgctttt tattggaacg
     1561 ggatttgcgg ttagtgactt acccaaaccg ctaaagccga aaataattat cacaaaacca
     1621 atcaattcaa aagctacacc gattgcatca attgtgttat tgccaagaaa aatatcctcg
     1681 gaagatatca ggattaatcc accaagtaac aagaactgaa ttgcaacaag taagcgacct
     1741 ttagcatgat tgtccattta aaattccttg gatttttttg tactaaaaaa gtaactattt
     1801 ttaatattct acgcctggta gttgtgcgaa atgtccgcac aaagtgtgag tttatagaca
     1861 tgtttttcta gtattttcaa ggtttttcag gcgtagtttg ttaaatgtag gagcataaca
     1921 aagtgttaat gcactaaata acagaaaggc attaaacaat gaaaaataac aagctaatcg
     1981 gcttgatcgc catcgtcgta ggtgcaattt ttatcgttgc aggtggcaca acttacaaca
     2041 tggttcataa caaactagct gacgaaaaga ttacagtttc tgctgatgcc gagcgctatg
     2101 ccggtcagca agtggcagga ccatttactg cgtatacaga ggccatgatt attcagaagc
     2161 atgcccttga ggcaacaggc ggaaaaacct atgcggaact agatcgtgag gacccactac
     2221 gtgcagtagc aatgaacggc tccttcctac gtgcttcatt gttcacctca gtggtgtcct
     2281 tcggagttgc cgcacttgct gctggcttag gcgtaatgtt cgttcttctt ggggtggcca
     2341 ttaggaatac tgccaggtag caaaaaacgc agatagcggc tgtgatctcg ggtttacggc
     2401 cgctatcggt ttttctagtg atttaatgag ttatggcaaa aaatgaatct gcaaaagacc
     2461 cagccaaagc tgcattcttg gccgccttag agaaaaagaa aaactccgca acgaaaaagg
     2521 ctggcgaaag tcacgctgaa ggtaaaggtg cggcaaaagg tcccaactct ggtgtcacca
     2581 gaagaataga acgccgccgt agcggtagtt catagtaatt agttcgaaga caattctcgc
     2641 cgagcctaat tttataacca agggtatatt tggttgatgg ttgtcaaaga aattcggtcc
     2701 gcaagtaacg tcattggtcc gattcttgct gccattgcag tcatttattt ttggaataac
     2761 acacctaatg cattagaagt tatttttatc agcattgtgc ttattggtgg agttaccact
     2821 gccgttcatc acgctgaaat aatttctgct tatgttggcc gttccctcgg tgcgttaatt
     2881 cttgccttgt cggtaacaat tatcgaagtc ggcgtaattg tggcaattat gacttcgtca
     2941 gatggtgctg catcaacact tggccgggat acagttttcg cagcggtaat gatcacctgc
     3001 aatggaataa tcggcttttc ggtagtagct gcctcattga aacaagcggc accaacgttt
     3061 aactccgaag gtagcggcgc ggcattaagt gccattgtca caattgccac gttaagcctg
     3121 gtattgccaa cattcactcg aagcagttcg ggcccgactt taactactgg ccagttaacc
     3181 tttgtttcga ttatttcgtt ggcggtgtac gcgctgtttc tatacgtact gaccgtacga
     3241 aatcgtgaac attttgaaga tcccgaaggt caagttgtag tgccctacgc aattcatcca
     3301 acaaaacgag tttttcgaca aagtgtttca ctacttctta tttcattaac tgcaatcatt
     3361 ggcttagcaa aagtcatgtc gccttccatc gaaagtgttg tgcgagatgc tggctttccg
     3421 ctttcagtgg ttgcggttgt ggtagcgcta gtaattcttg ctccagaagg tattgcggcg
     3481 gttaaatcct caagtcgagg tgatttgcag actggattta atcttgccta tggatccgct
     3541 ttagcaagta ttggcttaac tattccagcc ctagcaatag cctccacctt gcttggcata
     3601 gaactacaac ttggtttagg tgcaactgaa atagttcttt acgtattaac tttgctagta
     3661 agccttgtca ctgtgtcttc acatcgcact acgttctttc aaggtggctt acatttagtt
     3721 atattctcgg catttatctt tttggcacta agcccataaa agttttataa attttgcttg
     3781 agtagttcgc ccaatatcga aataccttta accaagcgtt catcagaaat tccagaaaaa
     3841 ctaaagcggg catgatttga ctcttgctca actgggaaga atgtagcgcc tggaacaaaa
     3901 gctactttgc cctgcgggat cagtatttct ttcataaatt ttgttgcatc aaactcttta
     3961 ggaaaagtta gccaggtgaa caagccgccc tctggtttcg taaaagttac ctcgcttgga
     4021 aatgcctcta ccattgttgc ggccattaag tctcgtttat gccggtagac cggtctgact
     4081 ttttctagat gttcatcaat gttgtatcgg cgtaaatact ctaaagtcgc attcatattt
     4141 aatgtgctgt tttgggtgtc agcagcaagt tttaaaagcg aaagtttttc taatatttct
     4201 ggcgaagcta acgcccatcc cagccgcatt gatggtgaaa gaatttttga aaaactgccc
     4261 agatgaagta ctcggccttg cgtatccata cttttcagag taggtaacga tgttccggca
     4321 aagcggagct cacggtatgg cgtatcttca accacaaaaa tattaa
//
LOCUS       DBNHVN010000318         4479 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1992779, whole genome shotgun sequence.
ACCESSION   DBNHVN010000318 DBNHVN010000000
VERSION     DBNHVN010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1992779"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..118)
                     /locus_tag="AC16Y1_06065"
     CDS             complement(<1..118)
                     /locus_tag="AC16Y1_06065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009884049.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit C is part of the
                     membrane proton channel F0; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit C"
                     /protein_id="HIR5832058.1"
                     /translation="MQLLAEINGNLNAIGYGLAAIGPGIGIGILVGKALEGIA"
     gene            complement(160..981)
                     /gene="atpB"
                     /locus_tag="AC16Y1_06070"
     CDS             complement(160..981)
                     /gene="atpB"
                     /locus_tag="AC16Y1_06070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010157575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR5832059.1"
                     /translation="MLNTIAHLPSTFSFAEGEGGGFKPPSVGEFFPAPILFEGTIFEF
                     NRINMIGVIMTLLLCSFFIAAFRKPQFVPRGVQNLGELAVEFVQKNIIEEILGHKGPR
                     YAVYLTSMFWLLFFFNITGILPLMHISVTSVIGIPLLLAGISWIVFNVEGIRANGVGH
                     YLKSNLFPAGVPAPIYALVTPIEFVSTFVLRPVTLTIRLLANMMAGHLLLVLFFGATQ
                     YFLLESDGFMKVVAIPAFFMGFAFTLFEILVAFLQAYIFTLLTAVYIDGATSAEH"
     gene            complement(1001..1240)
                     /locus_tag="AC16Y1_06075"
     CDS             complement(1001..1240)
                     /locus_tag="AC16Y1_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HIR5832060.1"
                     /translation="MTGENLPKFDPAKAGPGEDMAWRMISTLLAGPIVWGAIGALVDR
                     VADTSRVFLPLGVVIGFITSIYIVYVRFGKDSESK"
     gene            complement(1307..1723)
                     /locus_tag="AC16Y1_06080"
     CDS             complement(1307..1723)
                     /locus_tag="AC16Y1_06080"
                     /inference="COORDINATES: protein motif:HMM:NF053722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cg1360 family ATP-synthase-associated protein"
                     /protein_id="HIR5832061.1"
                     /translation="MGQVTAKQAARRAALFGLLAWLAITVIALRIGDATEGEAGFWGS
                     ALGAIVAGVFFSVTALIALATTNMGPDKLGFVILGSWLVKIIALMGVLAWLRNQDFYS
                     RPFFFGVLVVQTMVLLVLEAIMLTRAKVPYVEPNKD"
     gene            complement(1755..2843)
                     /locus_tag="AC16Y1_06085"
     CDS             complement(1755..2843)
                     /locus_tag="AC16Y1_06085"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HIR5832062.1"
                     /translation="MREYLLVLLAAAAVTYLVTPVARAMALKFGALAEVRDRDVHEEP
                     TPRWGGLAMFVGLAVSILLATQLPLMSTIFSDNSQINALLSGAAIIVILGVVDDKIGL
                     DAPTKLVGQILAAGTMAMQGVTLVWLPIGGTFILDPTTSVFVTVLIVLVSINAVNMVD
                     GLDGLATSIVGVGAAAFFSYSYFLSVVNGYQRATLATLVSATLMGMCAGFLPHNWFRA
                     RVFMGDTGSMLIGFLMAASSIMLTGQIDPGGLTSGSLLPAFLPIVLPLSILAIPLLDL
                     ILAILRRSRRGRSPFQPDKEHIHHRLLKLGHGQERAVLIMTAFTGLIAFSAVSLAFIP
                     AWITAMVFALATIALVGWILNPPKVSYK"
     gene            complement(2879..4144)
                     /locus_tag="AC16Y1_06090"
     CDS             complement(2879..4144)
                     /locus_tag="AC16Y1_06090"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010851616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR5832063.1"
                     /translation="MSETFIGSDFDELKNTDPEIAQVLIDELNRLRTGIQLIASENFT
                     SPAVLTALGSTLSNKYAEGYPGKRYYGGCEVVDVAETLGIERAKKLFGADHANLQPHS
                     GASANIAVFGAFLKPGDTYLAMSLPHGGHLTHGAAVSYSGKWFNAVHYGVAEGTEDID
                     YDQLRDLAREHKPKMIIAGGSAIPRLIDFKAFREIADEVGAIFHVDAAHFIGLVAGKA
                     IPSPVPYADVVTFTTHKVLRGPRSGMIVCKEEHAAAIDKAVFPMMQGGPMMHVVAAKA
                     VALKECMTPEYQKYAKDVIANAQELVAGLAQEGMRPVTGGTDTHLALLDLQGIDVSGK
                     DAEARCGASGIVLNKNSIPYDPKPAAVTSGVRVGTPSLTTQGMGIPEMKQIAKLIGRA
                     VKSDDSSVHAEIKAEVNTLTAAFPAYPRG"
     gene            complement(4213..>4479)
                     /locus_tag="AC16Y1_06095"
     CDS             complement(4213..>4479)
                     /locus_tag="AC16Y1_06095"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR5832064.1"
                     /translation="QSLLHRVGPLAMTGAQLSGQEPIQMVEQAVAALGQKVAVYLDAG
                     ALSHNVSSVIDATTSNLRLIRQGAITIGSIREVNPNVIDATVTK"
BASE COUNT         1297 a         1080 c          981 g         1121 t
ORIGIN      
        1 gagcgatgcc ttctagtgcc ttaccgacaa gaatgccgat accgatacca gggccaatcg
       61 cggccaagcc atagccaatt gcgtttaggt tgccattgat ttctgcgagc aactgcacgt
      121 tatttcctct cgttttgcga aatttctttc gcggtttttt tagtgttccg cactagtggc
      181 gccgtctatg tagacggcag tcaatagtgt gaagatgtat gcctgtagga atgccaccaa
      241 gatttcaaaa agggtgaaag caaagcccat aaagaacgcg gggattgcca ccaccttcat
      301 gaaaccatct gattccaaca gaaaatattg ggttgcccca aagaataaaa ctaaaagtaa
      361 gtgtccggcc atcatattgg ctaacagacg gattgttaac gtcactggac gaagcacgaa
      421 agttgaaaca aattcaattg gtgtcaccaa tgcatagata ggtgcaggca caccagctgg
      481 gaacaagttg ctttttaggt agtgccctac accgttagct ctaattcctt cgacgttaaa
      541 aactatccat gaaataccag caagtagaag cggaattccg ataacgctgg taactgaaat
      601 atgcattagt ggcaggatgc cagtgatgtt aaagaagaaa agcaaccaaa acattgaagt
      661 taggtaaacg gcatatcgtg gacctttgtg gccgagaatt tcttcaataa tatttttttg
      721 cacaaattca acagctagct cacctaagtt ttgaacacca cgtggaacaa attgaggctt
      781 gcggaaggct gcaataaaga aactacaaag cagcagtgtc atgatgacac caatcatgtt
      841 tattcgattg aactcaaaaa tagttccttc gaaaagtatt ggggcgggaa agaattcacc
      901 tactgatggt ggcttaaaac cgccaccttc tccttcagca aaggaaaacg ttgacggtaa
      961 gtgagcgatg gtgtttagca cggcgcttac ttaactccct ttatttagat tcggaatctt
     1021 tcccgaatct gacgtacaca atgtagatag aagtgatgaa cccaatcacc acaccaagcg
     1081 ggaggaagac ccttgatgta tctgcgactc ggtcgaccag tgctccgatt gcgccccaaa
     1141 cgatcgggcc agcaagtaaa gtgctgatca tccgccaggc catatcctcg cctggtccag
     1201 cttttgccgg atcaaactta ggtaggtttt cccctgtcat cgaaatcgaa tttagcagag
     1261 aatcccaagc cgtgattcac cggtccgcct caagaaaagg acgcaattaa tccttgtttg
     1321 gctcgacata tggcaccttg gcccgagtca gcattattgc ctctaggact aaaagcacca
     1381 tagtttggac cactaaaaca ccaaagaaga aagggcgact ataaaagtcc tgattgcgca
     1441 gccaggccaa aactcccatg agcgcaatta ttttcactag ccaggagcca agaatcacaa
     1501 agccgagttt gtctggcccc atgtttgtag tggcaagtgc gattagtgca gtaacactaa
     1561 aaaaaacacc cgcgacaatt gcacctaagg cactgcccca aaatccagct tctccttctg
     1621 tggcatcgcc aatccgaagt gcgataacgg taattgcaag ccaagcaagt agtccaaaaa
     1681 gtgccgcacg acgagctgct tgctttgcag taacttgacc catgattact tccctagtcg
     1741 caatttatcg aactttattt atacgaaact tttggtggat taagtatcca gcccactaat
     1801 gcaattgtgg caagcgcgaa caccatcgcg gtgatccagg ctggaataaa cgccaggcta
     1861 accgctgaga atgcaattag cccagtgaaa gcagtcatga ttaaaactgc acgttcttgc
     1921 ccatgtccaa gttttaacaa gcggtgatga atgtgctctt tatctggctg gaatggagat
     1981 ctgcctcttc ggcttcggcg caaaattgcc aaaattaaat caagcagtgg gattgccaaa
     2041 attgaaagtg gcaaaacaat tggcaagaat gctggcaata atgaaccgct ggtcaatcca
     2101 ccgggatcaa tttgtccggt caacataatg gacgaagcag ccatcagaaa cccaattaac
     2161 attgagccgg tatcgcccat gaaaacccgg gcgcgaaacc agttatgcgg caagaaacca
     2221 gcgcacattc ccatcaacgt tgctgagacc aaggtggcta acgtagctcg ctgatagcca
     2281 ttcaccacac ttaaaaagta tgaatacgaa aagaatgctg ccgcgccaac gcccacaatg
     2341 ctggtggcca ggccatcaag accatcaacc atattcacag cattgatact gactaaaaca
     2401 attaacactg tgacaaaaac agaggtggtc ggatccaaaa taaatgtgcc gccaattggt
     2461 agccaaacta gtgtgacacc ttgcattgcc atggttccag ctgcaagtat ttgcccaact
     2521 aatttcgttg gagcatcgag accgattttg tcatctacga cgccaagaat cacgataatt
     2581 gcggctccac taagcaacgc attaatttga gaattatcac taaaaattgt gctcattaac
     2641 ggcaattggg ttgctaacaa aattgaaaca gctaacccga cgaacattgc caagccaccc
     2701 caacgtggcg ttggctcttc gtggacatct cgatcgcgaa cctcagccaa tgcgccgaat
     2761 ttaagtgcca ttgcgcgcgc gacaggagtt accagataag taacagcagc ggcagctagc
     2821 agcacaagta agtattcgcg cacaggtcaa cgctacttgg tcaacttagt tgggcaaatt
     2881 atccgcgtgg ataggcagga aatgcagcag ttaaagtatt aacttcggcc tttatttctg
     2941 catgcacaga tgaatcgtct gacttaacag ctcgaccaat cagttttgca atctgcttca
     3001 tttccggaat gcccatacct tgagtagtta acgaaggcgt gccaactcga acaccacttg
     3061 tcactgcggc tggttttgga tcgtatggga ttgaattttt atttagcaca attcctgatg
     3121 ctccacaacg tgcctcggcg tctttgcccg acacatcaat accctgtaga tcaagtaatg
     3181 caagatgtgt atctgttcca ccagtaacag gacgcatacc ttcttgggcc aaaccggcga
     3241 caagttcttg agcatttgca attacatctt ttgcatactt ttgatattca ggcgtcatac
     3301 attctttaag cgcaacagct ttagcagcca ccacatgcat cattgggcca ccttgcatca
     3361 ttgggaatac cgccttatcg attgcggctg catgttcttc tttgcaaacg atcattccgc
     3421 ttcgtgggcc acgaagcact ttatgtgtcg tgaaagttac tacatccgcg taaggaactg
     3481 gcgaagggat tgctttacca gctactagac cgatgaagtg ggctgcatca acgtggaaga
     3541 ttgccccaac ttcatctgca atttcacgga atgctttgaa gtcaattagg cgtggaattg
     3601 cggaaccacc agcgataatc atctttggtt tatgttctcg cgctaaatcc cgtaactggt
     3661 cgtagtcaat atcttctgtt ccttcagcta caccgtagtg aactgcgtta aaccatttac
     3721 ccgaataaga aacagcagcg ccgtgggtta aatgaccacc atgtggcaaa gacattgcca
     3781 agtaagtgtc acctggtttt agaaatgcac caaagactgc aatgtttgcc gatgctcccg
     3841 agtggggctg caaattagcg tgatctgcac caaataattt cttggcccgc tcaataccaa
     3901 gggtttccgc gacgtcgact acttcacaac cgccgtagta gcgtttgccc ggatagcctt
     3961 cagcatattt gttactcaaa gttgaaccca atgcagtaag taccgctggt gaagtgaaat
     4021 tctcgcttgc gattaactga attccggttc taagtcgatt tagttcatct atcaaaacct
     4081 gagctatttc tggatcagta tttttaagtt catcaaaatc agaaccaata aatgtttcac
     4141 tcatcgcatt acctaacgcc ttgattgatt agtaccaccc gaaaagtagt tagatcagtt
     4201 tagaaccaat gactactttg tgacagtcgc gtcgataaca tttggattta cttctcgaat
     4261 ggatccaata gtgattgcac cctggcgaat tagtcttaag tttgaagtag ttgcatcaat
     4321 aacggaagac acattgtgag atagcgctcc agcgtcaagg taaaccgcaa ctttttgacc
     4381 aagcgctgcc acagcttgtt ccaccatttg gattggctcc tgtccactta gctgtgcacc
     4441 agtcatcgca agtggaccga cgcggtgtaa aagtgactg
//
LOCUS       DBNHVN010000319         1279 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1620610, whole genome shotgun sequence.
ACCESSION   DBNHVN010000319 DBNHVN010000000
VERSION     DBNHVN010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1620610"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..725
                     /locus_tag="AC16Y1_06100"
     CDS             120..725
                     /locus_tag="AC16Y1_06100"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020488415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HIR5832065.1"
                     /translation="MSTNSTVASISSEAIEQLFTQAHTAYNFIDFEVDDQLLEQIYDL
                     TKMAPSSMNAQPLRVTYIKSAEAKARLLPLIPESNRNKSKSASVVAILSADLDFHEHF
                     NVLQPHNPNAKNNFTDPVKREQFARFNAAIQAGYFILAVRAVGLDAGPMAGVNLAGID
                     QEFLANTNQKTLFVVNIGKVSETGKFPRSGRLSFAQAAKIL"
     gene            758..1249
                     /locus_tag="AC16Y1_06105"
     CDS             758..1249
                     /locus_tag="AC16Y1_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015659860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR5832066.1"
                     /translation="MAKSDEVDALVAAWQRERPDLDVTLMHVLSRVSRLARHLDLARR
                     EAFAAHDLEAGEFDVLAALRRAGKPYELTPSQLIAANLVTSGTMTNRIDRLEQKNLVT
                     RKPDPNDGRGVLVKLTPTGQNTVDAALTDLLERERILLAGISKSERNQLADVLREIVL
                     PFD"
BASE COUNT          385 a          266 c          276 g          352 t
ORIGIN      
        1 ccacggcgca agagtaattc gttaagtcaa atttaaacct gttcgctttt agagaataaa
       61 gttgtttgaa aattcaacta aatctaaact cagtttatta attcaaaatt aggaatcaca
      121 tgagtacgaa ttcaaccgtc gcttcaatca gcagcgaagc tatcgagcaa ctttttacgc
      181 aagcacacac cgcctataac tttattgatt tcgaagttga cgaccagtta cttgagcaaa
      241 tttatgatct aacaaaaatg gcgcctagtt cgatgaatgc gcaaccgctg cgcgttactt
      301 acatcaaaag tgctgaggct aaagccagac ttttgccgtt gattccagaa agtaaccgta
      361 ataaatcaaa aagtgcttca gttgttgcga ttctttctgc ggatttagat tttcacgagc
      421 actttaacgt tttgcaaccg cacaatccga atgccaaaaa taatttcact gacccagtaa
      481 aaagagagca atttgctcgg ttcaatgcgg caatccaggc aggttatttt attttggctg
      541 ttcgagccgt tggtttagat gctggtccaa tggctggcgt taacttagct ggaattgatc
      601 aagaattttt agccaataca aatcaaaaaa ctttgtttgt agtaaacatt ggcaaagtaa
      661 gtgagactgg aaaatttcca cgaagtggtc gcttaagttt tgcgcaagca gcaaagattc
      721 tctgagtcat ttgcgcctcg aaccgtagga ttacttcgtg gcaaagtccg atgaagtcga
      781 tgcattagtt gcagcctggc agcgcgagcg ccctgacttg gatgtaactc ttatgcatgt
      841 tcttagccgt gtaagccgat tagcaagaca tctagaccta gcaaggcggg aagctttcgc
      901 tgcacacgat cttgaagccg gagagtttga tgtgctagct gcacttcggc gcgcaggtaa
      961 accttatgaa ttaactccat cacaactaat tgccgccaac ttagtgacta gtggcacgat
     1021 gacaaaccga attgatcgac tagaacaaaa aaatttagtc acccgcaaac ctgatccaaa
     1081 tgatggccgc ggagttttag ttaaattaac tcccactggg caaaatacag tggacgccgc
     1141 acttactgac ttgctcgagc gcgaacggat tttgttagct gggatttcaa aaagtgaacg
     1201 aaatcaactt gctgacgtat tgcgtgaaat tgttttaccg tttgattaag aaaaaattag
     1261 cgcctggtaa ttaatcggg
//
LOCUS       DBNHVN010000320         4653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_878894, whole genome shotgun sequence.
ACCESSION   DBNHVN010000320 DBNHVN010000000
VERSION     DBNHVN010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_878894"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="AC16Y1_06110"
     CDS             complement(<1..183)
                     /locus_tag="AC16Y1_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5832067.1"
                     /translation="MKIDRGTAVFVVLASGVLFGTTGTATLIFDVEASSTSFAATRLL
                     VGALGLVAISIIQRGLP"
     gene            602..1156
                     /locus_tag="AC16Y1_06115"
     CDS             602..1156
                     /locus_tag="AC16Y1_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019852313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HIR5832068.1"
                     /translation="MTTKSGDRESYFPAIEKKYGQPMRYWFDVMADLKDAKYPEQIAY
                     LKENHGFSQSHANALVMYSRGSKSSKRFSTLDQYLKPFDETKRKTVQSIFKAIQTKYP
                     KMELVMAWNQPMLKLGNEYIFGVSVLKNHILLGPWGKDSVQKLEKQLVDYKTNKKTIQ
                     VPTDWKIDKKLLLDLVAIRLKEIK"
     gene            complement(1158..1997)
                     /locus_tag="AC16Y1_06120"
     CDS             complement(1158..1997)
                     /locus_tag="AC16Y1_06120"
                     /inference="COORDINATES: protein motif:HMM:NF022112.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HIR5832069.1"
                     /translation="MKKVALTIAVTVFLGGCAMSDTNTSPIPAQSSAISAEPSQAPSS
                     AENQAILYFVSDTGKSFRLFSEQHDLISSSGDLVTDALATLLSGVKPKDSDYTNLWSS
                     DSRLNGLTVNNGFATVDLHFEQLNVGAETELRAIEQILWTLKVNNPEISKVEFLRDGN
                     IVESFAGHVDTLDVFQIDEGYQSLATIDLDLDEGAVIKSGDPVTGLACTFEANAPWEL
                     FKDGQLISSGAETAEMACPVRSKFSISLGPLKPGSYLLRVWESSMEDGSLINEDTKNL
                     VVK"
     gene            2093..2254
                     /locus_tag="AC16Y1_06125"
     CDS             2093..2254
                     /locus_tag="AC16Y1_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5832070.1"
                     /translation="MINPFFILRILIVLVVALIVYRKTKNFKKTFFAVLLSSTLLWGF
                     AFIVDQFME"
     gene            2261..2875
                     /locus_tag="AC16Y1_06130"
     CDS             2261..2875
                     /locus_tag="AC16Y1_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5832071.1"
                     /translation="MARTLLLTALVLAINLIYFAIQQNARKRAQFAKSFALWMMIGFG
                     LLGGLFTAGYALADPGGSTGSLLVALWAVPLIVLSVLAWLKSEIVKPALWILLIAAGV
                     TNLVSLIFTEEYREFFNERGPWLAIGSFAFTITATVFGYHVNARLGGWMNIAIALMPI
                     AMSILAFDLQYVLAVSSIAAALTPGFTAGILLLISDRFAKASFI"
     gene            complement(2868..3611)
                     /locus_tag="AC16Y1_06135"
     CDS             complement(2868..3611)
                     /locus_tag="AC16Y1_06135"
                     /inference="COORDINATES: protein motif:HMM:NF021505.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HIR5832072.1"
                     /translation="MNILALDLINGLPAHPLIVHAAVVLVPITALFFIALVIFPKLQK
                     NYLNLVILGAVLGLGSSLAAKQSGEELAERVGLPVEHAQFGERLVIVSGALLALIVAF
                     KYLTRSENDSKNLLRIVGVVGALVAIVAIVLTYQTGDSGAKSVWEDRIAGVTEATPSA
                     TPSESAAEPTGISLAEVALHNSPEDCWSVVNGKVYDLTKFVSQHPGGADKISGICGRD
                     GTSAFNGQHRGEDTPVSTLGNYLIGDLAK"
     gene            complement(3646..>4653)
                     /locus_tag="AC16Y1_06140"
     CDS             complement(3646..>4653)
                     /locus_tag="AC16Y1_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HIR5832073.1"
                     /translation="VKATVDAYDGTVKIYGWDESDPILQTWMKAFPGVVQPKSAIPAG
                     VLDHVRYPEDMFKVQRDILSRYHVNDAQAFYSGQDFWIIPKDPTKPELQQAQPPYYLT
                     LKMPGQTEAAFQLTTTYAPNKRQTLASFMAVNADHGPDYGTIRILQLPRNTTIPGPTQ
                     VQNNFESDPTVATQLSLLRQGGSDVQLGNLLSLPVGGGLLYFEPVYVRGSQGEGYPLL
                     RKVLVSFGSKVAFEDDLATALSKVFTGQTVTPTPESPETPTSPEEGQTLTDLQILAKA
                     ISDANAAYEDGQAALAKGDFAAYGIAQDRLADALRRISSASGTITGVSPIPSASPTPT
                     P"
BASE COUNT         1341 a         1022 c          961 g         1329 t
ORIGIN      
        1 aggcaagccg cgttgaatta tcgaaatggc caccaagcca agtgcaccaa ctagtaatcg
       61 tgtggctgca aatgacgttg aacttgcttc aacgtcaaaa attaaagtag ctgtaccagt
      121 agtgccaaaa agtactcccg atgccaaaac tacaaatacg gcagtaccgc gatcaatttt
      181 caaatctact tacctatcaa cagtcgactt aagtttaaca accatatatt tcataaacta
      241 aaagtgatcc cctataaaaa actgagcagc tgaacaagta gttcagctgc tcagttgcga
      301 aaataatcag atcagagagg taactgatta ttttctactt tcggccgacg ttctcaacac
      361 tttctagaac tagattccag taacggcttg cgtctagttt catcgccacg tacacatttg
      421 gctcttgatc tgaggtaaga ataatccgac ttccggaatc tgatgcagag atcttctaag
      481 ttcttgagtg taagaaaaat tttctgaaac cacgcgcttt acgtcactaa gttccataaa
      541 tctccaaatg tttaaattga aagtatcaat ttaactcacg ttaacatgat aattttcatt
      601 agtgactact aaaagcggcg atcgtgaatc ctacttcccg gcaatagaga aaaaatatgg
      661 acagccaatg cgctattggt ttgacgttat ggctgaccta aaggatgcga aataccctga
      721 gcaaattgct tatttaaagg aaaatcacgg ctttagccaa tcccatgcga atgcacttgt
      781 tatgtactcg cgtgggtcta aaagttctaa gcggttttct acgttagacc agtaccttaa
      841 accctttgat gaaaccaagc gcaaaactgt acaaagtatc tttaaagcaa tccaaactaa
      901 atatccaaaa atggaattag taatggcttg gaaccaacca atgctaaaac ttggcaatga
      961 atacattttt ggcgttagcg tactaaaaaa ccatattttg cttggacctt ggggcaaaga
     1021 ctcagtccaa aaacttgaaa agcaattagt cgactacaaa acaaataaaa aaactattca
     1081 ggttccaacc gactggaaaa ttgataagaa attactgctt gatttagtcg caatccgctt
     1141 aaaagaaatt aagtgactta cttaactaca agattcttag tgtcttcatt tattaatgag
     1201 ccatcttcca tcgaagattc ccaaacccgt aacaagtaac ttcctggctt tagcggccca
     1261 aggctgattg agaattttga tctgactggg catgccatct cggctgtttc tgcacctgag
     1321 ctaattaact gtccatcttt gaataattcc caaggtgcat tcgcctcaaa cgtacaggct
     1381 aatccagtga ccggatcgcc acttttgatt acggcaccct catcaagatc tagatcaata
     1441 gttgctagcg attgatatcc ttcatcaatt tgaaaaacat ccagagtgtc aacatgccca
     1501 gcaaaactct caacaatgtt gccatcacgc aaaaattcca ctttggatat ctctggatta
     1561 tttaccttta gcgtccacaa gatttgttca atggctcgca gttctgtttc tgcgccgacg
     1621 ttcaattgct caaaatgtaa gtcgacggtg gcgaatccat tattaacagt caagccattt
     1681 aaccttgaat cactactcca caggttcgtg taatcagaat ctttaggttt tacacctgac
     1741 aaaagagttg ccagggcatc ggttactaaa tcaccactag aagaaatcaa gtcatgttgc
     1801 tcagaaaata gtcgaaacga tttgcctgtg tcactgacga aatacaaaat cgcctgattc
     1861 tctgctgagg atggcgcctg gcttggctcg gctgaaatcg cactgctttg agcagggata
     1921 ggtgaagtgt ttgtatcgct catcgcacag ccaccaagaa ataccgtaac tgcaatggtc
     1981 aaagcaactt tcttcatgaa tccaatatgc cagtaaaacc acatattgct ggctttgacg
     2041 ctgatctcga tgaaaattta ctgatggttt agatttgaag tagaggtgca atgtgattaa
     2101 tccatttttt attttacgaa ttttaattgt gctagtcgtt gcgctaattg tttaccgcaa
     2161 aaccaaaaat ttcaagaaga ctttttttgc agttttgctg tcaagcacat tgctctgggg
     2221 ttttgctttt atcgttgatc aatttatgga ataggtagaa atggctcgga cattattatt
     2281 aaccgcttta gtactagcaa taaatcttat ttactttgca attcagcaaa atgcgcggaa
     2341 gcgcgctcaa tttgcaaagt catttgcact ttggatgatg attggttttg gacttttggg
     2401 tgggttgttc accgcaggat atgcgcttgc agatccaggt ggttctacag gctcattact
     2461 tgttgcactg tgggcggtac cgctaattgt gctatccgta ctcgcctggc ttaagtcaga
     2521 aattgttaag ccagcccttt ggatactttt aattgctgca ggtgtaacaa atttagttag
     2581 cctgattttc acagaagagt accgtgaatt ttttaatgag cgtggtccat ggcttgcaat
     2641 cggtagcttt gctttcacta tcacagcaac cgtttttggt taccatgtga acgcacgtct
     2701 tggtggctgg atgaacatcg cgatcgcgct aatgccaatt gccatgtcta tcttggcttt
     2761 tgatttgcaa tatgtacttg cggttagctc aatcgcagca gctttaactc ccgggtttac
     2821 tgcaggaatt ttgctcttga ttagtgacag atttgctaaa gctagtttta tttagctaaa
     2881 tcgccaatca agtaatttcc aagcgtactt accggagtat cttcgccgcg gtgctggcca
     2941 ttaaatgcag acgtgccgtc gcgaccacaa attccggaaa ttttgtcagc gccacctggg
     3001 tgttggctaa cgaatttagt taaatcgtaa actttgccat taaccactga ccagcaatct
     3061 tcgggggagt tatgcagtgc tacttctgcc aacgaaattc cagtcggctc agcagctgac
     3121 tcagacggtg ttgcggacgg agtggcttca gttactccag cgatgcgatc ttcccaaaca
     3181 gattttgcgc cagaatctcc agtttgatat gtaagaacta ttgcaacaat cgctactagt
     3241 gcgccaacta ctccgactat tcgcagtaaa tttttggagt cattctcgga tcttgttaaa
     3301 tacttaaaag caactatcag cgcaagtaaa gcaccggaaa caattactaa tctctcgcca
     3361 aactgcgcat gctctactgg taatccaaca cgttcggcca gttcctcacc tgattgttta
     3421 gccgcaagcg aagaaccaag accaagaacg gcgccaagta ttactagatt taggtaattt
     3481 ttttgaagtt taggaaaaat taccaaagca atgaagaaca acgcggttat cggcaccaaa
     3541 actacagctg catgaacgat tagtgggtgt gcaggcaggc cattaatcaa atcaagggca
     3601 aggatattca taactgtctg tccttttcgt ttttacatca atgatttatg gagttggcgt
     3661 aggactagca cttggaatcg gacttactcc ggtgattgtt ccactcgcac ttgaaatccg
     3721 tcgcaatgca tctgcaagtc gatcttgcgc tattccataa gcagcaaagt cacctttagc
     3781 caaggctgct tggccatctt cgtaggcagc atttgcatca gaaattgcct tagccaaaat
     3841 ttgcaaatca gttagcgtct gaccttcttc tggcgaggtt ggtgtctcag gggattcagg
     3901 tgttggcgtt actgtttgcc cagtgaaaac tttgcttaac gcagtggcta aatcatcttc
     3961 gaaggcaact ttagaaccga acgaaacaag tactttccga agtagcggat aaccttcacc
     4021 ctgactacct cgaacataaa caggttcgaa gtaaagtaag ccgccaccaa caggcaggga
     4081 aagcaggtta cctaactgaa catctgatcc accttgacga agcagtgaca actgggttgc
     4141 cacggttggg tcagactcga aattattttg tacttgggtt ggccccggaa tagttgtatt
     4201 tcgcggtaac tgcagaattc gaatcgttcc ataatcaggg ccatgatctg catttaccgc
     4261 catgaatgac gctaaagttt ggcgcttatt cggtgcataa gttgtcgtca actggaaggc
     4321 agcctcagtc tgtccaggca tctttaacgt taagtaataa ggtggctgcg cttgctgcaa
     4381 ttctggctta gttgggtctt taggaataat ccagaaatcc tgaccagagt agaaagcttg
     4441 ggcatcgttc acgtggtagc gagacagaat atcgcgctga actttaaaca tatcttctgg
     4501 gtaacgaacg tgatcaagaa cacctgcagg tattgcagac ttaggctgaa caacccctgg
     4561 gaatgctttc atccaagttt gcaaaattgg atctgattca tcccaaccat aaatcttgac
     4621 cgtaccgtcg taagcatcaa ccgtagcttt aac
//
LOCUS       DBNHVN010000321        10351 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_730513, whole genome shotgun sequence.
ACCESSION   DBNHVN010000321 DBNHVN010000000
VERSION     DBNHVN010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 10351)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10351)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10351
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_730513"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="AC16Y1_06145"
     CDS             <1..546
                     /locus_tag="AC16Y1_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5832074.1"
                     /translation="LLVYLNHAVQILDSGIATKQDIDAAMRDLAQYPMGPLELVDLIG
                     IDTCVEILNTIYQESGNDLHKPAAGLIHLLSKGNKGRKSGSGFYDYATHHLPPTSENE
                     NIKVAIFTELETAYLGNAVAMEASGYASKADIDNGMKLGCGLPKGPFERIDLIGLESL
                     RERQAALAASTGNPQFAPLSM"
     gene            566..844
                     /locus_tag="AC16Y1_06150"
     CDS             566..844
                     /locus_tag="AC16Y1_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005458710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5832075.1"
                     /translation="MGRRNRREPIPEPGELSVGGFRRSETHTDGEWIVQSITGATSVK
                     AYRCPGCDMEIKPATPHIVAWPIDAVDDRRHWHSACWTKRNHRRPIRK"
     gene            841..1596
                     /locus_tag="AC16Y1_06155"
     CDS             841..1596
                     /locus_tag="AC16Y1_06155"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007464471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5832076.1"
                     /translation="MISEISANTVLPAQREQITLQTEDGLALVGELALPESNPPKATI
                     ICLHPNPTGGGMMDSHLYRKAAWRLPALAELAVLRFNTRGTTSATGTSEGEYDEGRGE
                     GFDLRAALQFAIDRKLPNIWLVGWSFGTDVTLLHGNVDPVRGAILFSPPLKWSGVDAL
                     ENWAESGRPLVALVPELDEYLQPDEARERFAVIPQCEVIAIPECKHLWIGEKFVRIAW
                     DEALKRIRPELPTLTWTWTGPAQKWDDLTGKLT"
     gene            1611..2393
                     /locus_tag="AC16Y1_06160"
     CDS             1611..2393
                     /locus_tag="AC16Y1_06160"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5832077.1"
                     /translation="MNKYGATENSKTGRNILLIHPFPFDSRLWSEVAQKLSQKNYVLV
                     PDLRGCGKSNLGTDEPSLNLLAKDILELVETEKVSDVILAGISLGGYVAMEISRMKQE
                     VLSGLILLDTKACADSTAAKENRLRIATQMESGAKVSLFAEQMLESVVGNYTLENRSD
                     VVALVKNWMLDASPQTIAWLQRVMASRPESFSTLGKLDVPVLLIRGAQDGISSVEDFA
                     QMQKNLRQVTYIEISNAGHLPPVEDPVATFAAIDQWLTTFAN"
     gene            complement(2377..3135)
                     /locus_tag="AC16Y1_06165"
     CDS             complement(2377..3135)
                     /locus_tag="AC16Y1_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5832078.1"
                     /translation="MAKWRLLFMSLPVVAIVLGLRYLVHNVAGINDVVSFSDAGAVLT
                     GASIIVGLMLGGVIADYKESEKLPAALAGGLSGFEGLAIKGLEAKGLDTSWVHPRIVK
                     TGTAINDWLYGRISDDEVFAAVGDNAQLIVDLEKAGVGTHYLSRLFVANGDLGGALSR
                     MSVIRNTNFIKSGYALMEILIAIVLGLLIIVDYPKPTVQWFVSGVLAAAYTYLVLLVK
                     DLDNPFDYGANGEKGSAADVDLSPWKKVFANLQK"
     gene            complement(3155..3592)
                     /locus_tag="AC16Y1_06170"
     CDS             complement(3155..3592)
                     /locus_tag="AC16Y1_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5832079.1"
                     /translation="MPAPQQVIRDLIIGIDHVGMAVSNLAESIDYWSVNFGAQLHSRE
                     INEEQGIEEALLEFPDKSQIQLLASVDEHSAISKFLTKHGQGVQQIALRVTDLTAATA
                     ALAESNIPMVYPAPKSGSNNTTINFIHPKHAGGVLLELVEYPA"
     gene            3672..4853
                     /locus_tag="AC16Y1_06175"
     CDS             3672..4853
                     /locus_tag="AC16Y1_06175"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR5832080.1"
                     /translation="MHMSVIVGGARTAIGRLLGSLKDFSASDLGSIAIKGALAKTGLN
                     PNSVDYVIMGHVLQAGAGQNSARQASVKAGLPMSVPALTVNKVCLSGMNAISLADQMI
                     RSGECQVVIAGGMESMSQAPHLLIGSREGVKYGDWQLKDSMTLDGLFCAIDQLAMGES
                     TEKYNSNYKLTRQAQDEFSVRSHTLVKEAIDSGKFAEEIVPVTIPTKSGPDLVFQVDE
                     GVRPETSIATLSQLKPAFAKDGTITAASSSQISDGAAALIVMSEEKAVELGLPILAHI
                     EAQGMVAGPDPSLHAQPANAIKVAASKAGIKINEIDLFEINEAFAAVSLVSTSELGVS
                     SDRVNVNGGAIALGHPIGMSGARLVLHLAMELKRRGGGLGAAALCGGGGQGDAIIIRV
                     P"
     gene            4881..5813
                     /locus_tag="AC16Y1_06180"
     CDS             4881..5813
                     /locus_tag="AC16Y1_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5832081.1"
                     /translation="MTQNPMNLQGAVDLGALAAVRESQEKASLVQANMAANPLANKVI
                     LDVTTADFEQLVINQSANVPVVIDLWATWCGPCKQLSPILEQLALEYNGKFVLAKIDV
                     DAEPQISAAFQVQSIPTVFVAIGGQVAPLFQGAQPYPQVKQVIDAVLAQAKQLGVTGS
                     LAETPEEIQQEVDQPISDPRFDAAETALEAGDWDGALAAYQQVLSATPNDPIAKIGVL
                     NVALLKRTDGIDLTNAAANPDSSIESQILAADAQFLLNDLAGAFTRLIELVKVTSGKD
                     REQVRDRLVELFDIIGPTDPSVVKARTQLANALF"
     gene            complement(5820..7973)
                     /gene="glgB"
                     /locus_tag="AC16Y1_06185"
     CDS             complement(5820..7973)
                     /gene="glgB"
                     /locus_tag="AC16Y1_06185"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020579296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIR5832082.1"
                     /translation="MAIHKAPTEQLDQIVDGWHHDPHSILGAHPDGNGIIIRVLRPWA
                     KAVQMVIDGQAHNLIHEHRGIWLGRIAGTEIPSYTLLVDYGDGFIKADDAFRFIPTIG
                     ELDLHLFNEGRHEQLWNFLGAHQISHQGVSGVRFAVWAPNAQGVRVVGDFNHWDGLAT
                     PMRSMGASGVWELFIPLIESGAIYRYSILGKDGIWRMKSDPMAYATEAPPANASKVFK
                     SNYAWQDQDWLTRRGQVNPHDQAMSLYEVHLGSWRPGLTYLELAKQLVDYTVEHGFTH
                     IEFMPVAEHPFSPSWGYQVTSYFAPTSRFGNPDEFRYLVDACHQAGLGVIMDWVPAHF
                     PKDDWALARFDGTCLYEHENPKRGEHPDWGTLVFDFGRNEVRNFLVANALYWLTEFHI
                     DGLRVDAVSSMLYLDYSREDGQWESNIHGGRENLEAMQFLQEVTATVYGRVPGAVMIA
                     EESTAWPGVTKTTDSGGLGFGFKWNMGWMHDSLEYIKEDPINRKYHHDKMTFSLMYAW
                     SENFILPISHDEVVHGKGSLYARMPGDHWQKLANLRAYLGFMWAHPGKQLIFMGTEFA
                     QPDEWNESQSLAWHLTELDQHEGVQKTIKDLNKNYRETPSLWQRDNEPSGFEWLVGDD
                     AANNVFAWARHGNDGSTLVSVTNFSPVVREDYQLPFPSSGIWQQILNTDDLKYGGSGV
                     GSIDHLEVAAVNYFGKPASAIVTLPPLATCWFKSK"
     gene            complement(7973..9967)
                     /locus_tag="AC16Y1_06190"
     CDS             complement(7973..9967)
                     /locus_tag="AC16Y1_06190"
                     /EC_number="2.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003987854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009313 - oligosaccharide catabolic process
                     [Evidence IEA];
                     GO_process: GO:0030979 - alpha-glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HIR5832083.1"
                     /translation="MATTKPSSGSIGRIPVLDIWPMVSGGIRPVKAVTAERIKIYASV
                     FREGHDSVAADVVLLNPAGEQFSRTRMKLIGEGTDRYVAQIQPDKEGLWSYRIEAWSD
                     PLSTWLHRIEVKIPANIDVELEFQEGTTLLLKIAKLASGKDAETIRKIAKSINDKNLL
                     PAIRQKLIEDPIFLETLRSYPLREFVSSYGPIKLKVERKRALFGSWYEFFPRSEGAKQ
                     LTDGSWQSGTFKTAALRLPAIAEMGFDVLYLPPIHPIGVTNRKGPNNSLTAGAKDPGS
                     PWAVGSKSGGHDAIHSDLGTEKDFVDFVNAARKLNIEIALDLALQASPDHPWVSSHPE
                     WFTSRADGTIAYAENPPKKYQDIYPINFDQDPDGIYAEVERIVRHWMNLGVRIFRVDN
                     PHTKPVAFWEWLIGRINATDPDVIFLAEAFTRPAMMRVLAEIGFQQSYTYFTWRNTKW
                     ELEEYLGELSGGASPYMRPNFFVNTPDILPAYLQEGGRPAFAIRAALAATLSPTWGVY
                     AGYELYENQPTKPGSEEYYFSEKYEYKNRDWAGALKSGNSLAPFIKKLNEIRRSHDAL
                     QQLREIEFHRVDSDQVICYSKKDGADLVVCVVTLDPFVSQKTTVHFDMPKLGLDWASQ
                     VDFLDELTGNEIKISEHTVFNLDPSGQVAFILTKKKSVKR"
     gene            complement(10030..>10351)
                     /locus_tag="AC16Y1_06195"
     CDS             complement(10030..>10351)
                     /locus_tag="AC16Y1_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5832084.1"
                     /translation="WFNADGNLFSEENWQDQQTRAIGMYLAGAISDGINTDIVDNGFY
                     YFLNSSDNPIMVNLPAGDFATSYRLKFNTVDSRDWEKFDEITAGSQVTLARWSSALWM
                     VTSR"
BASE COUNT         2956 a         2292 c         2279 g         2824 t
ORIGIN      
        1 ttacttgttt atctaaacca tgctgtgcaa attttagatt caggaattgc gaccaaacaa
       61 gatattgatg cggccatgag agatctagca caatatccga tgggaccact ggagttagtg
      121 gacttaatag gtattgatac ttgcgtggaa atcctaaata ccatttacca ggaatcagga
      181 aatgatttgc acaaaccagc tgctggccta atccatttac tttctaaagg aaataaagga
      241 cgaaagtcgg gaagtggctt ttacgattat gcgacacatc atttgccacc aactagtgaa
      301 aatgaaaaca taaaagtcgc tattttcact gaacttgaga cggcatattt aggtaacgca
      361 gttgcgatgg aagcaagcgg ttatgcaagt aaagcggata ttgataacgg aatgaaactc
      421 ggttgtggtt tgccaaaagg tccatttgaa cgaattgatc taattggact tgagtctctg
      481 cgcgaaaggc aagcagcact tgctgctagt actggaaacc cgcagtttgc tccgttatct
      541 atgtagcgca acttaaactt aagacatggg tcgccgaaat cgccgagaac caattcctga
      601 accaggtgaa cttagcgttg gcggatttcg cagatcagaa acacatacag atggcgaatg
      661 gatagttcaa tcaattactg gagcaacttc ggttaaggca tatcggtgtc ctgggtgcga
      721 catggaaatt aaaccagcta ctccacacat agtcgcatgg ccaattgatg cggtagatga
      781 tcgtcgacat tggcatagtg catgttggac taaacgaaat caccgtaggc cgatccgcaa
      841 atgatcagcg aaatttcagc gaacacagtt ttacctgcac agcgcgaaca aattactctg
      901 cagactgaag atggtttagc gttagtgggc gagttagcgc tgccagaaag taatccgcca
      961 aaagccacaa ttatttgcct tcatccaaac ccaactggtg gcggaatgat ggatagtcac
     1021 ttgtatcgaa aagctgcctg gcggttacca gcccttgccg aactggccgt tttaaggttc
     1081 aacacgcgcg gcacaacaag tgcaactgga acgagcgaag gtgaatatga cgaaggtcgc
     1141 ggtgaaggtt ttgatcttag agctgcactc caattcgcga tagatcggaa actgccaaat
     1201 atttggttag tcggatggtc gtttggaaca gatgtgactt tattgcatgg caatgttgat
     1261 ccggttcgag gagccatttt gtttagcccg ccacttaagt ggagtggcgt agacgcttta
     1321 gaaaattggg cagaatcggg tcgaccgctt gttgcacttg tgccagaact cgacgaatac
     1381 ttacagccag atgaagcaag agaacgtttt gccgtgattc cacagtgtga agttattgcg
     1441 atccctgaat gtaagcacct ctggattggg gaaaaattcg ttcggattgc atgggatgag
     1501 gcacttaaac gtattcgacc tgaattaccg accttaactt ggacgtggac tggtccagca
     1561 cagaaatggg atgacttaac tggaaagtta acttaagcgt gcaactccag gtgaataaat
     1621 acggtgcaac cgaaaattcg aaaactggaa gaaacattct cttaattcat ccatttcctt
     1681 tcgatagccg actttggtct gaggtagccc aaaaactcag ccagaaaaat tatgtactag
     1741 tgccagattt gagaggctgc ggtaaaagta acttaggcac ggatgaacca agcttaaatt
     1801 tattggctaa agacattctt gaattggttg agactgaaaa ggtatcggat gtaattttgg
     1861 ccggtatttc acttggtggc tatgtcgcta tggaaatttc tcgaatgaaa caagaagttc
     1921 taagtggact aatacttttg gataccaaag cttgcgcgga ctcgactgct gcaaaagaaa
     1981 accgcctacg tattgccacc caaatggaat ctggagcaaa agtttcacta tttgccgagc
     2041 aaatgctcga aagcgttgta ggtaattaca cacttgaaaa ccggtcagat gtagttgcgc
     2101 tagtaaaaaa ctggatgctt gatgccagcc cgcaaacaat tgcctggctg caacgagtca
     2161 tggcatcgcg acctgaaagt ttttcgactc tcgggaaatt agatgttccg gtcttattaa
     2221 ttagaggggc gcaggatgga attagttcag ttgaagattt cgcgcagatg cagaaaaact
     2281 taaggcaagt gacctacatt gaaatttcaa acgcaggtca cttgccgcca gttgaagacc
     2341 cagttgccac atttgcggca atagatcagt ggttaactac ttttgcaaat tagcaaaaac
     2401 tttcttccaa ggtgatagat caacatctgc tgctgagcct ttttcgccat ttgcaccgta
     2461 gtcaaatggg ttatctagat cttttactag aagtactaaa tatgtataag ccgcagctag
     2521 gacaccagaa acaaaccatt gcacagtcgg ttttggataa tcaacaatta ttaacagacc
     2581 cagcacaatc gcaatcaaga tttccattaa ggcataacca cttttaataa agttggtatt
     2641 acgaataaca gacatccgag aaagagctcc acctaaatca ccattggcaa caaaaagcct
     2701 ggatagataa tgtgtgccta caccagcttt ttctaaatca acaattagct gagcattatc
     2761 gccaacagca gcaaacactt catcatcgct gatacgtccg tatagccagt cgttaattgc
     2821 agttccagtt ttaacaatcc ttgggtggac ccaagaagta tctaatcctt ttgcttctaa
     2881 gcctttgatc gctaaacctt caaagcctga aagcccaccg gctagcgcag ctggcaattt
     2941 ctcggattct ttgtagtccg caataacacc accaagcatt aaaccaacaa tgatcgatgc
     3001 gccagttaat actgcgccgg catcagaaaa actgactaca tcattaattc cagcaacatt
     3061 gtggactagg taacgtagcc caaggactat cgctacaaca ggtaaggaca tgaaaagtag
     3121 acgccacttg gccatttatt ttccttttta ttgattaggc aggatattca acgagctcga
     3181 gaagcactcc acctgcatgt ttcgggtgaa taaagtttat cgtggtgttg tttgagccag
     3241 atttaggcgc agggtaaacc atcggaatat tgctttctgc tagcgcagca gtggcggcag
     3301 taaggtcagt gactcttagc gcaatttgtt gtacgccttg accgtgttta gtaagaaatt
     3361 tactaattgc tgagtgctca tcaactgagg caagtagttg aatctgagat ttatccggga
     3421 attcaaggag tgcctcttca ataccctgct cttcattaat ttcacgcgaa tgtaactgcg
     3481 cgccaaaatt taccgaccag tagtcaattg actcagctaa gttacttacg gccataccca
     3541 cgtgatctat gccgataatc aagtcccgaa ttacttgctg tggcgcaggc atttgatccc
     3601 cttatttaac tcgctaaagt gacagtaggg gattttgccc taaacaggca atttgaatat
     3661 cgactgaaag gttgcacatg tcagtaatag ttggcggtgc acggacagca attggtcggt
     3721 tactcggaag tttaaaagat ttctcggcca gcgatcttgg atcaattgcc ataaaagggg
     3781 cgttagcaaa aactggcctt aatccaaatt cagttgacta cgtaatcatg ggtcatgtac
     3841 tgcaggctgg tgctgggcag aattcggctc gtcaggcatc agttaaagct gggttgccta
     3901 tgagcgttcc agcattgaca gttaataaag tttgcctctc tgggatgaat gcaatttcac
     3961 ttgccgacca aatgattcgt tcgggtgaat gccaagtggt tattgctggt ggcatggaaa
     4021 gtatgtcgca ggcaccacac ttacttatcg gaagccgtga aggtgttaag tatggtgact
     4081 ggcagttaaa agactcaatg acacttgatg gattattttg cgcaatagac cagctagcca
     4141 tgggcgagtc caccgaaaaa tataattcaa actataaatt aactcgacaa gcgcaagatg
     4201 aattttcggt tcgttcccac actcttgtta aagaagctat tgattcgggt aaatttgccg
     4261 aagaaatagt tccagtaacg atcccaacta aatctggacc tgatttagtt tttcaagttg
     4321 atgaaggtgt cagacctgaa actagtattg caactctttc acaattaaaa cccgcgtttg
     4381 ccaaagatgg aacaataacc gcggcttcgt cgtcgcagat ctcagatggc gcagcagcac
     4441 taattgtgat gagcgaagaa aaagcagtcg aattaggatt gccgatcctg gcgcacattg
     4501 aagcgcaagg aatggtggcc ggtccagatc cttcgctaca cgcccaacct gcaaatgcaa
     4561 ttaaagttgc agcgagcaag gccgggataa aaattaatga gattgacctg tttgagatca
     4621 atgaggcctt tgccgccgta tcacttgtta gcacttctga attgggcgta agtagtgatc
     4681 gagttaatgt caatggcgga gcaattgcgc tcggccatcc aattggaatg tccggtgcca
     4741 gacttgtgtt gcatttagcg atggaattaa aacgtcgcgg cggtggcctc ggcgctgccg
     4801 cgttatgcgg cggcggtggc caaggtgacg caataattat tcgcgttcct tgatcagata
     4861 taactaaacg gagaaattaa gtgactcaaa atccaatgaa cttgcaaggc gctgttgatt
     4921 taggtgcgct tgcggcagtt agggaatcgc aagaaaaggc aagtcttgtc caggccaata
     4981 tggctgctaa cccattggct aacaaagtta tcctggatgt aacgaccgct gattttgagc
     5041 agttagttat caaccaatct gcaaatgttc cagtggttat tgatttgtgg gcgacttggt
     5101 gtggcccgtg caaacaactt tctccgatcc ttgaacagtt agcactcgag tacaacggaa
     5161 aatttgtgtt agcgaaaatt gatgttgatg cagaaccgca aattagtgca gcatttcaag
     5221 tgcaatcaat tccaacggta ttcgttgcaa ttggcggaca agtagcgcca cttttccaag
     5281 gtgcccaacc ttatccgcag gtaaagcaag ttatcgacgc agttttggcc caagcaaaac
     5341 aacttggtgt cactggatcc cttgctgaaa cgccagaaga aatacagcaa gaagttgatc
     5401 aaccaatctc tgacccaaga tttgatgcag cagagacagc gcttgaagca ggggattggg
     5461 atggtgcctt agctgcatat cagcaagttt tgtcagccac tccaaatgat ccaatcgcaa
     5521 aaattggtgt tttgaatgtt gcgttactaa agcgtacgga tggaattgat ttaactaacg
     5581 cggcagcaaa cccagattca tcaatagaaa gccaaatttt ggcagctgat gctcagttct
     5641 tgttgaacga tttggccggc gcatttactc ggctaatcga gttagtcaaa gtgacatcgg
     5701 gtaaagatcg agagcaagtt cgggatcgct tagttgagct tttcgacatt attggaccaa
     5761 ctgacccttc agttgtaaaa gcaagaactc aacttgcaaa tgcacttttt taattaaaac
     5821 tattttgatt taaaccaaca agtagccaat gggggaagag tcactattgc gctggctggt
     5881 ttgccgaaat aattcaccgc agccacctct aaatggtcaa ttgatcccac cccagatcca
     5941 ccatatttaa gatcatcggt atttaggatt tgctgccaaa tgccagaact tggaaacgga
     6001 agttgataat cttcacgaac tactggcgaa aaattagtga ctgaaactag tgttgagccg
     6061 tcatttccgt gtcttgccca agcaaaaacg ttattcgctg cgtcatcacc aacgagccac
     6121 tcaaatccac ttggttcgtt atcacgttgc cacagacttg gagtttctcg atagtttttg
     6181 tttaaatcct taattgtctt ttgaacacct tcatgctgat caagctcggt taaatgccag
     6241 gccaaacttt gcgattcatt ccattcatcc ggttgtgcaa actcggtacc cataaaaatt
     6301 aattgtttac ctggatgtgc ccacataaaa cctaagtagg cgcgtaagtt tgctaacttc
     6361 tgccagtggt caccgggcat acgggcgtaa agagaacctt taccatgcac aacttcatca
     6421 tggcttatag gcaaaataaa attttcactc caggcataca taagtgaaaa agtcattttg
     6481 tcgtgatgat acttacgatt tatcggatct tcttttatgt attccagtga gtcgtgcatc
     6541 caacccatat tccatttgaa accaaaacct aagccacctg agtcggttgt tttagttaca
     6601 cccggccagg cggttgattc ttcggcaatc attacagccc ctggaacacg accataaaca
     6661 gtcgctgtaa cttcttgtaa aaactgcatg gcctctaaat tttctcgacc accatgaatg
     6721 tttgattccc attggccgtc ttcgcgtgaa taatccagat agagcattga cgaaacggcg
     6781 tctacacgta gcccatcaat gtgaaattca gttaaccaat aaagtgcgtt tgcaactaag
     6841 aaatttcgca cttcatttcg accaaaatcg aaaactagtg ttccccagtc aggatgttca
     6901 ccccgttttg gattttcatg ttcgtaaaga caagtgccat caaatctggc taacgcccaa
     6961 tcgtccttgg ggaaatgcgc tggaacccaa tccataatga caccaagtcc ggcttgatga
     7021 caggcgtcaa ctaagtatct gaattcatcc ggattaccaa aacgcgaagt tggggcaaag
     7081 taagaagtaa cttggtaacc ccatgaaggt gaaaatggat gttcggcaac gggcataaat
     7141 tcaatgtggg taaagccatg ttctactgtg taatcaacca actgctttgc gagttctaaa
     7201 taagttagtc ctggccgcca tgaaccaagg tgtacttcgt ataacgacat agcctggtcg
     7261 tgtggattaa cttgaccgcg cctggttaac caatcttgat cctgccaggc gtaatttgat
     7321 ttaaaaactt tcgaagcatt tgctggagga gcttcagtcg cgtaagccat cggatcagac
     7381 ttcatccgcc aaattccatc tttacccaaa attgaataac ggtaaattgc gccactttca
     7441 attagcggaa taaataattc ccaaactcca cttgcaccca tggatcgcat cggagtagcc
     7501 aggccatccc aatgattaaa gtcaccaact actcgaacgc cctgcgcatt tggcgcccac
     7561 actgcgaacc tgactccact aactccttgg tgacttattt gatgtgcacc taggaagttc
     7621 cacaattgtt cgtgtcgacc ttcattaaat aagtgcagat ctaattctcc gatggttgga
     7681 atgaatctga atgcgtcatc tgccttgata aatccatcgc cataatcaac aagcaatgtg
     7741 tagctgggta tttcagttcc agctatccga ccaagccaaa tcccgcgatg ttcgtggatg
     7801 agattatgcg cctgcccatc gatgaccatt tgcacggctt ttgcccaagg tcgtaagacc
     7861 cgaattatta tcccgttgcc atctggatgt gctcccaaaa ttgaatgtgg atcatgatgc
     7921 cagccatcaa caatctgatc caattgttca gttggagctt tgtgtattgc catcagcgtt
     7981 taacagactt tttcttagtc aaaataaatg ccacctgtcc agagggatca aggttaaata
     8041 cagtgtgctc ggaaatcttt atctcatttc cagttaactc atccaggaaa tcaacttggc
     8101 tagcccaatc aagacccagt tttggcatat caaagtgcac tgtggttttt tggctaacga
     8161 aaggatcaag agtcaccaca catacaacta gatcagcacc atcttttttc gaatagcaga
     8221 ttacctgatc ggaatcgact cgatgaaatt caatttcgcg taattgctgt aacgcatcgt
     8281 gcgatcttcg aatttcattt agtttcttaa tgaatggggc aagtgaattt ccagatttaa
     8341 gtgcgccagc ccaatctcga tttttgtact cgtatttttc ggaaaagtaa tactcttcac
     8401 ttcctggttt tgtcggttga ttttcataga gttcatagcc ggcataaacg ccccaggttg
     8461 gtgacaaggt tgcggctagg gctgctctta ttgcgaatgc cgggcggcca ccttcttgta
     8521 aatatgcagg aagtatgtcg ggggtattta caaagaagtt aggacgcata taaggcgaag
     8581 cgccgccgct taactcaccc aaatattctt caagttccca cttggtattt cgccaggtga
     8641 aataggtgta actctgctga aatccaattt cggcaagtac tcgcatcatt gctggcctag
     8701 tgaatgcctc agctaagaaa atcacatcag gatctgttgc attaattcga ccaattaacc
     8761 attcccagaa tgccactggc tttgtgtgcg gattatcgac tcgaaaaata cgcacaccta
     8821 aattcatcca atgtcgaacg attctttcta cttcggcata aataccgtca ggatcttgat
     8881 cgaagttaat tggataaata tcttgatatt tcttgggtgg gttttctgcg taagcgattg
     8941 tgccgtcagc tcggctggta aaccactctg gatgcgagct aacccaggga tgatctggac
     9001 ttgcttgcaa cgcgagatct aaggcaattt caatatttaa ttttcttgca gcattaacaa
     9061 aatcaacaaa atctttttcg gtacctaaat ctgaatgaat tgcgtcatgc ccacctgatt
     9121 tactaccaac tgcccaaggt gagccaggat cttttgcacc agcggttaag gaattattag
     9181 gaccttttcg attagtcact ccgattggat gaataggcgg cagatataaa acatcaaaac
     9241 ccatttctgc aatcgctggt aaccgaagcg cagctgtttt aaacgttcca ctttgccaag
     9301 aaccatcagt tagttgctta gcaccttcgc tgcgcggaaa aaattcatac catgatccaa
     9361 atagcgctcg ttttcgttcc acttttaatt taattggacc gtaacttgaa acaaattctc
     9421 ttaatggata gctacgtaac gtttccagga agattggatc ttcaattaac ttttgtctaa
     9481 tcgcgggcag taaatttttg tcattgattg attttgcgat ttttctaatc gtttcagcat
     9541 ctttacccga agctagtttg gcaattttga gtagcaaggt agtaccttct tgaaactcca
     9601 gctcaacatc gatgtttgct ggaattttta cttcgattcg atgtagccag gtagaaagtg
     9661 ggtcactcca agcttcaatt cggtaactcc aaagaccttc tttatcgggc tgaatctgtg
     9721 caacgtagcg atcagttccc tcgccgatta atttcattcg agttctagag aactgttcgc
     9781 cagcgggatt tagtaaaact acgtcagcag cgacactgtc atgtccttcg cgaaaaacac
     9841 tggcgtaaat ttttattctt tcggcggtga ccgctttaac cggacgaatt ccgccgctga
     9901 ccattggcca aatatctaaa actgggatcc gcccaattga tcctgaactt ggtttagtcg
     9961 tcgccacaag tcaaccgtag cgatttgcca ggcttcaggc gtaattgttg cccaattacc
    10021 tcaagttgtt tatcgagaag tgaccatcca tagtgcgcta gaccatctcg ctagcgtcac
    10081 ctgactacca gcagtaatct catcaaattt ctcccagtcg cgtgaatcaa cagtattgaa
    10141 cttgagccgg tagctagtcg caaaatcacc agctggcaga tttaccataa tcggattgtc
    10201 gcttgaattc aaaaagtaat agaagccatt atcaacaatg tcagtattta ttccgtcgga
    10261 aattgcgcca gcaaggtaca ttccaatcgc gcgcgtttgt tgatcctgcc agttttcttc
    10321 ggaaaataaa tttccatctg cgttaaacca g
//
LOCUS       DBNHVN010000322         3896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1251782, whole genome shotgun sequence.
ACCESSION   DBNHVN010000322 DBNHVN010000000
VERSION     DBNHVN010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1251782"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="AC16Y1_06200"
     CDS             <1..372
                     /locus_tag="AC16Y1_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006554466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HIR5832085.1"
                     /translation="YSEDEAPNTKGGVDTRVVMRFDKRLAPIKAAVLPLSRNADLSPK
                     ARDLATQLRKNWMIEFDDAGAIGRRYRRQDEIGTPFCITVDFETLTDQAVTVRDRDTM
                     AQERIGLDALEGWLAARLVGC"
     gene            372..1538
                     /gene="dusB"
                     /locus_tag="AC16Y1_06205"
     CDS             372..1538
                     /gene="dusB"
                     /locus_tag="AC16Y1_06205"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015936585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR5832086.1"
                     /translation="MFADILARNPVIELAPMAGITNRAFRRVCREASTELRAQAGQND
                     DDGTIYVAEMTTSQALLMRNQETLELVTFDSDEKLRSIQLYGVDPKIVGDAVNMVVSE
                     NMADHIDLNFGCPVPKVTRKGGGSALPWKRDLFAQIVQSAVKNAASKVPVTVKMRVGI
                     DHDHITYLDAGKIAAESGVAWVALHGRTAAQYYGGQADWSTIAKLVEHIPDTPVLGNG
                     DIWSAHDAKRMVEQTGVRGVVIGRGCLGRPWLFGQIRSAFAGQPIPSEPKAKDILRWM
                     RRHAQLLCEMHGDEFYGAREMRKHMAWYLKGIVAGSETRGSLALIDSLAQMDDLLATI
                     DPDQQLTEEIAHSPRGRTTPEKKVNLPDGWLNSQSMSQAEQATVREAELSVSGG"
     gene            1569..2798
                     /locus_tag="AC16Y1_06210"
     CDS             1569..2798
                     /locus_tag="AC16Y1_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013600459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HIR5832087.1"
                     /translation="MYQSEDLARWVPEPNKSVQRTEFARDRARIMHSSALRRLGVKTQ
                     VLVAAQDDFPRTRLTHSLEVAQVGRELGSALGCDPDLVDTACLAHDLGHPPFGHNGET
                     ALNEIASDIGGFEGNAQSFRLLTRLEPKAVSPSDGPRPNESIGLNLTRASLDAASKYP
                     WTKRATSNKFGVYDEDIDIFTWVRHGRIGDEVSFEAQVMDWSDDVAYCVHDVEDAIHE
                     SHVDVGILNSDAGRSEIVDIARQWYGLELEAAAVDEALIRLRSLVTWPTSYDGTLKSQ
                     AALKNLTSHLIGRFCLSVQRATQEKFGHTPLIRYGANLVVPQESRYEVTALKALAARF
                     VMTRAGADVVYERQRQFFADLYPIISSKPETHLDSIHLALWKSAPDERAQKRAIIDQL
                     ASLTDVSLTQWHSNLCN"
     gene            2808..>3896
                     /gene="dnaG"
                     /locus_tag="AC16Y1_06215"
     CDS             2808..>3896
                     /gene="dnaG"
                     /locus_tag="AC16Y1_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008405563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR5832088.1"
                     /translation="MAGRIKEDDVTAVRERARIEEIVRETVTLKAAGGGSFKGLCPFH
                     DERTPSFHVTPSKNLYHCFSCGEGGDVITFVRKMDALSFTEAVEKLAAKYSISLRYED
                     GPSGQASGQGQRSKLIEAHNLATKFFQDQLHKPEAQHARDFLTQRGFDQAAWELFGVG
                     YSPAGWDGLRTFLKGKGFTEAELLAGGLLVQGQKGAYDRFRDRVMWPIRDSGGDTIGF
                     GARKLTEADQGPKYLNTPETPIYRKSQVLYGIDLARRDMAKQQQAVVVEGYTDVMACH
                     LSGVTTAVATCGTAFGSEHVKVLRRILMDDEAKPASVVFTFDGDAAGKKAAIKAFTED
                     QKFAAATFVAVEPHGLDPCDLRLQNGQEA"
BASE COUNT         1065 a          853 c          981 g          997 t
ORIGIN      
        1 tactctgagg acgaggcacc aaatacaaaa ggtggcgttg atactcgagt tgttatgcgc
       61 tttgacaaga gactcgcacc aatcaaagct gcagttctgc ccctttctcg taatgcggat
      121 ctgtcgccaa aggcacgtga tttagcaact caattacgga aaaactggat gattgagttt
      181 gatgatgccg gtgcgattgg tcgtcgttac cgacgtcaag atgaaattgg tacgccattt
      241 tgcatcacgg ttgactttga gactttaact gatcaagcag tcaccgtccg cgatcgtgac
      301 acgatggcac aagaacgcat tggactggat gcgctagaag gatggctcgc cgctcgcctc
      361 gtaggttgct aatgtttgct gacatccttg ccagaaatcc agtgatcgaa ttagcgccaa
      421 tggccggcat cacaaaccgg gcatttcgtc gcgtttgccg agaggcatct actgaacttc
      481 gggcgcaggc tggccaaaat gatgacgatg gcactattta tgttgctgaa atgactacgt
      541 ctcaagcctt gctaatgcga aatcaggaaa ctttagaact agttactttt gattcagacg
      601 aaaagttgcg aagtatccag ctttatggcg ttgatccaaa aatagtaggc gacgcagtaa
      661 atatggtggt tagcgaaaat atggctgatc atattgattt aaattttggt tgccctgtac
      721 caaaagtcac gcggaaggga ggtgggagcg cacttccttg gaaacgagat ttattcgcac
      781 agattgttca atcagcagtc aaaaatgccg cgagtaaagt tccagtgact gtaaaaatgc
      841 gcgttggcat cgaccatgat cacatcactt atttggatgc tggaaaaatt gcagcggaat
      901 ctggcgtcgc gtgggttgca ttacacggcc gaacagcagc acagtattac ggcggacagg
      961 ccgattggtc cacgattgca aagttagttg agcacattcc tgatactccc gttttaggca
     1021 atggcgacat ctggtctgca catgacgcta aacggatggt cgaacaaact ggcgtccgag
     1081 gagtcgtgat cggaagaggc tgcttgggtc gaccctggct attcggacaa atcagaagtg
     1141 cttttgctgg tcaaccaatt ccgagtgaac ctaaagcaaa agatattttg cgttggatgc
     1201 gccgacatgc gcagttatta tgcgaaatgc atggtgatga attttatggt gcccgggaaa
     1261 tgcgcaaaca catggcttgg tatttaaaag gaattgttgc cggcagtgag acgcgtggct
     1321 cacttgcgct aattgattcg cttgcgcaga tggatgactt acttgccacc atcgatccag
     1381 accaacagct aaccgaggaa atcgcgcatt ccccacgggg ccgcacgaca ccagaaaaga
     1441 aagttaactt acccgacggt tggcttaatt cacaaagtat gtcgcaagct gaacaggcaa
     1501 ctgtacgtga ggctgaatta agtgtcagcg gcggctagtt aaatagaagt ggaaaataga
     1561 ttttgaatat gtaccagtcc gaagatctag cgcgttgggt gcccgaaccc aataagagtg
     1621 tacagcgcac agaatttgcg cgtgatcgag ctcgaattat gcatagctca gcgcttcgaa
     1681 ggcttggtgt aaaaactcaa gttttagtcg ctgctcaaga tgactttccg cgcactcgac
     1741 tcactcactc actggaagtg gctcaagtgg gacgtgaact ggggagtgct cttggttgtg
     1801 accctgattt agtcgacact gcctgtttag ctcatgattt aggccatcca ccttttgggc
     1861 acaacggtga aactgcgcta aatgaaattg caagtgatat aggtggattc gaaggtaacg
     1921 cacagtcatt tcgattatta accagacttg aaccaaaagc cgtaagtcca agtgatggtc
     1981 cgcggccaaa tgaatcaatt ggtttgaact taacccgggc atcactagat gcggcttcaa
     2041 aatatccatg gactaagcga gcaactagca acaaatttgg cgtttatgac gaagatatag
     2101 atattttcac ttgggtacgc cacggtcgaa taggtgatga agttagtttt gaagcccaag
     2161 ttatggattg gtctgacgat gttgcctact gtgtgcacga cgttgaagat gcaattcatg
     2221 aaagtcatgt tgatgtagga attcttaact cagatgcagg tcgctccgaa atagttgaca
     2281 ttgctcgcca gtggtacgga ttagaattag aagccgctgc agtagacgaa gcgttaatta
     2341 gattacgcag ccttgtaacc tggccaacaa gttatgacgg aacacttaaa tcgcaagcag
     2401 cactaaaaaa tttaaccagc catctaatcg gtcgcttttg cttatccgtc cagcgggcaa
     2461 cccaagaaaa atttgggcac accccgctaa ttcgttatgg cgctaatctc gttgtgccgc
     2521 aagaaagtcg ttacgaggta actgccctta aggcgcttgc tgctcgattt gtgatgacac
     2581 gagctggtgc agatgtggtt tatgagcgcc aacgacaatt ttttgctgac ctatatccga
     2641 ttatttcttc gaaacctgaa actcacctag attcaatcca tctggcgttg tggaaatctg
     2701 cacccgatga acgcgctcaa aagcgtgcaa ttattgatca attggcctca ctgactgacg
     2761 ttagcctcac tcagtggcat tcgaatcttt gcaattaggc ttacgttgtg gctgggcgaa
     2821 ttaaggaaga cgatgtaact gcagtacgcg aacgtgcccg gattgaagaa attgtccgag
     2881 aaacagtcac acttaaggcc gccggcggcg gttcgtttaa aggcctttgc ccatttcatg
     2941 atgaacgaac accttcattt cacgtaactc caagtaaaaa tctgtaccac tgtttttcat
     3001 gtggtgaagg cggagacgta atcactttcg tgcgaaagat ggatgcccta agtttcactg
     3061 aggcagttga gaaactagct gcaaaatatt caatcagttt gcgctatgaa gatggcccga
     3121 gtggtcaagc aagcggccag ggccagcgct ctaaattaat cgaggcgcat aatttagcga
     3181 ctaagttttt tcaagatcaa ttacacaaac ctgaagcaca gcatgctcgc gatttcttaa
     3241 cccaacgtgg atttgatcag gctgcttggg aattatttgg cgtcggctac tctccggctg
     3301 gttgggatgg acttcgcact tttctaaaag gcaagggatt tactgaagca gaattacttg
     3361 ctggtggact attagttcaa ggtcaaaagg gtgcttatga tcggtttcgc gatcgagtta
     3421 tgtggccaat tcgcgactct ggtggtgaca ccattggctt tggtgcgcgc aaacttaccg
     3481 aagctgatca agggccaaag tatttaaata ccccagaaac tccgatttat cgaaagtcgc
     3541 aagttttata tggcattgac ttagccagaa gagacatggc aaaacagcaa caagctgttg
     3601 ttgttgaagg ctataccgac gtaatggcct gtcatctatc tggagtaaca actgctgttg
     3661 caacttgtgg cacagctttt ggcagcgaac atgtcaaagt attgcgacgt attttgatgg
     3721 acgacgaagc caagcccgcc tcagttgttt ttacttttga cggagatgca gctggcaaaa
     3781 aggccgcaat aaaagcattc actgaagatc aaaaatttgc ggccgctaca ttcgtcgcag
     3841 ttgagccaca tggcttagat ccttgtgatc ttcgcctgca aaatggccaa gaagca
//
LOCUS       DBNHVN010000323         6072 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_655310, whole genome shotgun sequence.
ACCESSION   DBNHVN010000323 DBNHVN010000000
VERSION     DBNHVN010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6072)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6072)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6072
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_655310"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..694)
                     /locus_tag="AC16Y1_06220"
     CDS             complement(68..694)
                     /locus_tag="AC16Y1_06220"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5832089.1"
                     /translation="MITPDWFLNFEQVLPIAQHEHFSRFKFPEVIERNAAVLILFGPY
                     ENDADLLIIERAAHLIDHPGQPAFPGGHIESQDKSLIQTAFREAREEVGLNPETVRVV
                     AQLPKLWLPPSQVAVTPVVGWWEEPHELIEIDKNEVAGVHRIPILDLVNPENRVTVKT
                     RSGFLGPAFTVNNLVIWGFTGGIVSALLDIAGLTQEWDKSKLHELSFS"
     gene            complement(694..1389)
                     /gene="nth"
                     /locus_tag="AC16Y1_06225"
     CDS             complement(694..1389)
                     /gene="nth"
                     /locus_tag="AC16Y1_06225"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR5832090.1"
                     /translation="MNESRNTLIRRARKINGILGDVFPDAKCELDFKNPLELLVATVL
                     SAQCTDKRVNMVTPALFKKYPTAQKLAGAPRADLEELIKSTGFYRNKAASIHGLAAAL
                     LEKHGGKVPNELDALVQLPGVGRKTANVVLSNAFDIPGITVDTHVGRITRRLGFTTNT
                     DPVKVEFDLMEIFEPKDWKLLNHRLIFFGRRICHSRKPACGACPLAKLCPSFGEGPTD
                     KVQATKLLKMEDS"
     gene            complement(1429..2103)
                     /locus_tag="AC16Y1_06230"
     CDS             complement(1429..2103)
                     /locus_tag="AC16Y1_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003975365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR5832091.1"
                     /translation="MDENLRVAPLFKALDDETARNLRKSMVSQTLIKGQTLFQEGQPG
                     DRLYLVVNGKMKLSHSSGDGRESVFMVLGPGEMFGELSLFDPGPRTSTAIAVTDAEVL
                     GLSHTDIRPFLIEYPEVALSLLQAVAHRLRRTSEAMSDLVFADVPGRVAKALLELSEK
                     FGEKRASGIQVNHDLTQEELAQLVGASRETVNKALADFASRGWIRLETKSVELLDLDR
                     LAKRAK"
     gene            complement(2149..2613)
                     /locus_tag="AC16Y1_06235"
     CDS             complement(2149..2613)
                     /locus_tag="AC16Y1_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR5832092.1"
                     /translation="MSAVEDRLASNGHTLPEVAAPVAAYIPAVKSGNLVFTSGQLPSV
                     SGQLIAAGLVGGEVDPETANACARQCALNALAAIKSVVGDLDKVTRVVKVVGFVAAAP
                     GFTAIPPVINGASELFALAFGDNGVHARSAVGVAALPLNAPVEVEVIVEVKN"
     gene            complement(2621..2776)
                     /gene="sp36"
                     /locus_tag="AC16Y1_06240"
     CDS             complement(2621..2776)
                     /gene="sp36"
                     /locus_tag="AC16Y1_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012157776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sp36 family protein"
                     /protein_id="HIR5832093.1"
                     /translation="MTKWEYLTAPLLVHNTKAILDNFGEDGWELVAVIPGMNAENLVA
                     YFKRPKN"
     gene            complement(2823..3131)
                     /locus_tag="AC16Y1_06245"
     CDS             complement(2823..3131)
                     /locus_tag="AC16Y1_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HIR5832094.1"
                     /translation="MNWDSDWSRNAACRNTDPDLLFVQGAAQNRVKAICASCSVRTEC
                     LADALDNQIEFGVWGGMTERERRALLRRRSNVTSWRELLMSARNDYERLDPVAFVQGA
                     "
     gene            3341..3805
                     /locus_tag="AC16Y1_06250"
     CDS             3341..3805
                     /locus_tag="AC16Y1_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019355542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR5832095.1"
                     /translation="MGSVKDQIQSDLTSAIRAKDSLTAGTLRMVLAAITNEEVSGKEA
                     RTLTDQDLITVLNREAKKRKEASAAYIDAKRQDLADKEEAELKVIQNYLPEALSEEQL
                     AKIVADAVAQVTASGQSGPSAMGAVMKLVTPQIAGRADGGAVAAAVKAALAN"
     gene            3826..4758
                     /locus_tag="AC16Y1_06255"
     CDS             3826..4758
                     /locus_tag="AC16Y1_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR5832096.1"
                     /translation="MAKAAKSQVAIGLLIMYIAWGTTWVGIAYTVETMPPLIAMGLRF
                     LTATSIILLFISARNGFAAIKITKSQVKTAAILGVFMLAAGMGTATTGTKVVPIGVGS
                     LVVASMPIWIALFRFIHKDRPSKQTLIGLFFGFIGISLIMLPGQTQARPGAEGQNVLL
                     WMLVIMAGSISWGYTSYRSKNMDTPENAFLLSAYEMLFAGITLLFVGLILGESVNQFI
                     NASTASFGGWIYLIIVGSLLGFSTYVWLLDHAPISLVSTYTYVNPIVAIALGILIFNE
                     RLTTNVLIGGLIVLVAVAIVIRTESRNIEPAERV"
     gene            complement(4726..5796)
                     /gene="hisC"
                     /locus_tag="AC16Y1_06260"
     CDS             complement(4726..5796)
                     /gene="hisC"
                     /locus_tag="AC16Y1_06260"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012380056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 -
                     L-histidinol-phosphate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR5832097.1"
                     /translation="MEPQLRSVINDLPTYKAGKKVAGVAGLEPYKLSSNENPTAPLPS
                     VLKAISESALDINRYPDPFVTELTNALAKKFSVNPEFIATGTGSVGVCQQIMQAVAEP
                     GDEVIYAWRSFEAYPIITKIVGATPVEVPLTNGTEHDLDAMYQAITPKTKLIMVCTPN
                     NPTGAVVNHQKLKEFLTKVPKNILVVIDEAYVEFNQDDLAVRGLEFFTEFENVGVLRT
                     FSKAYGLAGLRIGYFIGPKNIAEAVRKTAVPFGVSSIAQSAALLSLEYEDELFKRVSE
                     MNSLRVWFEAELAKLGFKLPASQANFVWLPLGEKTDEFVAKCAQLAVAVRPFSGEGVR
                     ISIGEKPALKRVLNALSGFNIS"
     gene            complement(5826..>6072)
                     /locus_tag="AC16Y1_06265"
     CDS             complement(5826..>6072)
                     /locus_tag="AC16Y1_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014670665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR5832098.1"
                     /translation="ISQVGARAEYRFTKPAPVNGTQSAKASDDELDEFMHLFMCNRGI
                     LMTPFHNMALMCPTTTAAEVDQHAQLFEEAIRELVSS"
BASE COUNT         1691 a         1327 c         1257 g         1797 t
ORIGIN      
        1 acaaccaaac tgatcaatgc gatggcgatc agtaagaaat cgaggctgga aaatcctaaa
       61 ttcattatta tgaaaaactc agttcgtgta gtttgctttt atcccattct tgcgtaagtc
      121 ctgcaatgtc taataaggca gacactatcc cgccagtgaa accccaaatt actaaattat
      181 ttacggtaaa cgccggacca agaaagccac ttcgggtttt aaccgttact cgattttctg
      241 gattaactaa atcaagaatt ggaattcgat gaactccagc aacttcattt ttgtctatct
      301 caattaattc atgcggttct tcccaccaac caactacagg agttactgct acttgagatg
      361 gtggtaacca tagttttggt aactgagcca caacgcgaac tgtttcagga ttgagtccaa
      421 cttcttcacg tgcttctcga aatgctgttt gaattagcga tttatcttgt gactcaatgt
      481 gcccgccagg aaatgctggc tgaccaggat gatcgattaa gtgggcagct cgttcaataa
      541 tcagtaaatc tgcatcattt tcatatggac cgaacaaaat taatactgca gcatttcgct
      601 cgattacttc tggaaactta aaacgcgaga aatgttcatg ttgagcgatc ggcaacactt
      661 gctcaaaatt taaaaaccaa tctggcgtaa tcattaacta tcttccattt ttagcaattt
      721 ggttgcctgt actttgtcgg ttggaccttc accgaaagaa ggacagagct tggctaacgg
      781 acatgcaccg caggccggtt tgcgagaatg acaaatccga cggccaaaga aaattagtcg
      841 gtgatttagt aatttccaat cctttggttc gaaaatttcc atcaaatcaa attcaacttt
      901 tactgggtct gtgtttgttg taaaaccgag tcgccttgtt atccgaccta catgtgtatc
      961 aacagtgata ccgggaatat caaacgcgtt acttaacact acgtttgccg tcttgcggcc
     1021 aacacctgga agttggacaa gtgcatctag ttcgtttgga acttttccac cgtgcttttc
     1081 tagtaatgcg gcagctaaac catgaattga agcagcttta tttcgataga aaccagttga
     1141 cttaatcagc tcttcaagat ctgctctagg tgcacctgct aatttttgcg cggttggata
     1201 ttttttaaaa agtgctggag taaccatgtt tactcgctta tcagtgcact gcgcactcaa
     1261 aaccgttgcg actaaaagtt ctaatggatt tttaaaatcg agttcacact ttgcatctgg
     1321 aaaaacgtcg ccgagtatgc catttatttt gcgggctcga cgaattaatg tattgcgtga
     1381 ttcattcact taattagcct aagtcgttta agtgagggtt gaaactgctt acttagcgcg
     1441 cttagctaaa cgatctaagt caagtaattc aacagatttt gtttcaagtc tgatccaacc
     1501 tcggctagca aagtcagcta acgctttgtt tacggtttca cgcgatgctc cgactaactg
     1561 ggctaattct tcttgagtta gatcgtggtt gacttgaatt ccacttgcgc gcttttcgcc
     1621 aaacttttca ctaagttcaa gtagtgcttt agcgacccga cctggtacat cggcaaaaac
     1681 taaatcagac attgcttcac tagtcctgcg taatctgtgt gcgactgcct gcaaaagtga
     1741 aagtgcaact tctggatact caattaaaaa tggtcggatg tcggtgtgac ttaaacctag
     1801 gacttccgca tcggtaactg ctatcgcagt tgaagtgcgt ggtcctggat caaacaaaga
     1861 aagttcgcca aacatttcgc ccgggcccag caccataaat actgactctc gtccatcacc
     1921 cgatgaatgg ctaagcttca tttttccgtt aaccactaaa taaagacgat cgccaggctg
     1981 tccttcttgg aataaagttt ggcctttgat taaagtttgc gaaaccattg acttacgaag
     2041 gttgcgcgca gtttcatcgt ctaacgcttt aaaaagtggg gcgactcgta aattttcatc
     2101 catactgatc ctgctttaat ccacttgcta aatcattaac agtaaaattt agtttttcac
     2161 ttctacgatt acttcaactt ctactggtgc gtttagcgga agtgcggcta cgccaactgc
     2221 tgaccgagcg tgtactccgt tgtcaccaaa agcaagcgca aataattcac tggcaccgtt
     2281 aattactggc ggaattgccg taaacccagg agctgctgct acgaagccaa ccactttaac
     2341 tacccgagta actttgtcga gatcgccaac tactgacttg attgcagcaa gtgcgtttag
     2401 cgcgcactga cgagcacagg cgtttgcggt ttctggatct acttcgccgc caactaaacc
     2461 ggctgctatt aactgaccag aaacacttgg taattgtcca gatgtgaaaa ctaaattccc
     2521 tgacttaact gcaggaatgt aagcagcaac cggtgctgct acctctggca aagtgtgtcc
     2581 gttactggct aaccgatctt caactgcact cataacattc ctaattcttt gggcgcttga
     2641 aatacgcgac taaattttct gcattcattc ctggaataac ggcaactaac tcccagccat
     2701 cttcaccaaa attatccaaa attgcttttg tgttatgtac caacagtgga gcggttaagt
     2761 attcccattt agtcataacg aaactctacg ccaaaagtta tgacaagtaa taattggatc
     2821 cgttatgcgc cttggacaaa ggccactgga tcaagacgtt catagtcatt acgtgctgac
     2881 attaaaagtt cgcgccaaga agtaacgttt gatcggcgac gcaataatgc gcgacgttct
     2941 ctttcggtca tgccacccca cacaccgaat tcaatttggt tatctaaagc atctgctaaa
     3001 cattcagttc gcacactgca tgaagcgcaa attgctttca ctcggttctg tgctgcaccc
     3061 tgcacaaaaa gtaagtctgg gtctgtattt cgacaagctg cgttgcggct ccaatcgcta
     3121 tcccaattca cccggacaca ctccttgctt ttcaacttgc aactgaaaca actatttagg
     3181 ttgtcgcggg tctatgtcaa tacctctggc gcaactattt ttatcaagct tgtgcaagtt
     3241 ttcactaagt taaatctgaa tttagttacc gattgacagt tcttggttgc tatgcccacc
     3301 gcaattacat ctttatgaag caatctggca aactaagcgt gtgggttcag taaaagatca
     3361 aattcaaagt gacttaacat cagcaattcg cgctaaagat tcgttaactg caggtacatt
     3421 acggatggtt ttagcagcaa ttaccaatga agaggtttct ggtaaagaag ctagaacttt
     3481 aactgaccaa gatttaatta ctgtattaaa tcgtgaggcc aaaaagcgca aagaggcttc
     3541 ggctgcttac atcgacgcca aacgccaaga tctagccgac aaagaagaag ccgaattaaa
     3601 agttattcag aactatttac ctgaagcatt aagtgaagaa cagcttgcaa aaattgttgc
     3661 tgacgccgtt gcacaagtta cagctagcgg acagtcaggg ccaagtgcaa tgggtgcagt
     3721 aatgaaatta gtaactccac aaattgcggg tcgagccgat ggtggcgcag ttgctgccgc
     3781 cgttaaagct gcgctggcaa attaataaat tcgcaaagaa tataaatggc taaagctgct
     3841 aaatcccagg tcgcaattgg gctactgatt atgtacattg cctggggtac aacctgggtt
     3901 ggaatcgcgt acaccgtaga aacaatgcca ccgctgattg caatgggtct tagattctta
     3961 actgcaactt caataattct gttatttatt tcggcgcgaa atggatttgc ggcaataaaa
     4021 attacaaaat ctcaagtaaa gacggcggcc attctcggcg tatttatgtt ggctgccgga
     4081 atgggaactg caacaaccgg tacgaaggtt gtccctatcg gtgtgggttc gcttgtggtt
     4141 gcatcaatgc cgatttggat tgcgctcttt agatttattc acaaagatcg cccaagtaaa
     4201 caaactttaa ttggtttgtt ttttgggttt attggaatca gtttgatcat gttgccggga
     4261 caaacgcaag cacgaccggg tgccgaaggg caaaatgttt tgctttggat gttggtgatt
     4321 atggcgggct ctatttcttg gggttacacc tcataccgca gtaaaaatat ggacactcca
     4381 gaaaatgcat ttttattgag tgcttacgaa atgttatttg ctggaatcac attgcttttc
     4441 gttggtttaa tcctgggcga atcggttaat caatttatca atgcctcgac cgcgtcattt
     4501 ggtggttgga tttacttgat tattgttggc tcgttacttg gcttcagtac ctatgtttgg
     4561 ctacttgacc atgcaccaat atctttagtt tctacctaca catatgtaaa tccaatagtt
     4621 gcgattgcac ttggtatttt gattttcaat gaacgactaa ctacaaacgt tttgattggc
     4681 ggcttaattg ttttagtcgc ggtagcaatt gtgattagaa ctgaatcaag aaatattgaa
     4741 cccgctgagc gcgtttaata ctcgctttaa cgctggcttt tcgccgattg aaatccgaac
     4801 gccttcgcca ctaaaaggtc taactgcaac agcaagttgc gcacattttg cgacaaattc
     4861 atcagttttc tcaccaagtg gtaaccaaac aaaatttgcc tgactcgctg gtaatttaaa
     4921 accaagtttt gcaagttctg cttcaaacca aacgcgcaaa ctattcattt cactaactct
     4981 tttgaatagt tcatcttcgt actcaagtga aagtagtgct gctgattggg cgattgaaga
     5041 aacaccgaaa ggcacagctg ttttgcggac tgcttcggca atattttttg gtccgatgaa
     5101 atatccgatc cgcaagccgg ctaaaccata ggctttagaa aaagttctta agacacccac
     5161 gttttcaaat tctgtaaaaa attctaaacc gcgaactgct agatcatctt gattgaattc
     5221 aacataagcc tcatcaataa ctaccaaaat attttttggc acttttgtta aaaactcttt
     5281 tagtttttga tgattaacta ctgccccggt tggattattg ggggtgcaaa ccataatcaa
     5341 tttagttttt ggtgtgattg cttgatacat cgcatcaaga tcatgttcgg tgccattagt
     5401 tagtggtact tcaaccggag tagcaccaac gatttttgtg atgattgggt aagcctcaaa
     5461 tgatcgccaa gcatagataa cttcgtcacc gggctctgcc actgcttgca ttatttgttg
     5521 gcaaactcca actgatccgg tgccagttgc aataaattct ggattaacag agaacttctt
     5581 tgccagcgca tttgttaatt cagttacaaa tggatcagga tacctattta tatctaaggc
     5641 actttcagaa attgctttaa gcacacttgg taatggtgcg gtgggatttt cattacttga
     5701 aagtttgtat ggttcaagtc cagcaacgcc agcaactttc ttaccggctt tgtaagtggg
     5761 taagtcatta attaccgagc gaagttgcgg ttccacacct agagcctaaa ggtaatttgg
     5821 cttactcacg aactgacaag ttcgcgaatg gcttcttcaa ataactgtgc gtgctgatcg
     5881 acttcggctg ctgttgtagt tggacacata agcgccatgt tatgaaatgg cgtcattaaa
     5941 attccacgat tacacatgaa cagatgcatg aactcgtcta gctcatcgtc acttgctttt
     6001 gcagattgtg ttccatttac tggggcaggt ttagtgaaac gatattcagc ccgtgcacca
     6061 acttgagaga ta
//
LOCUS       DBNHVN010000324         4120 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1474717, whole genome shotgun sequence.
ACCESSION   DBNHVN010000324 DBNHVN010000000
VERSION     DBNHVN010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1474717"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="AC16Y1_06270"
     CDS             <1..348
                     /locus_tag="AC16Y1_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5832099.1"
                     /translation="LTFVTYLSLAWGIFINLAVASNQKFALPIAAGGKFENFPKAVRI
                     VYFIQSVWICYQTWIFYQLISGNTIKPSWIIFIFISLGLIGTLLNLISRSKFERLNAI
                     PLALITYSFWLLR"
     gene            complement(352..1059)
                     /locus_tag="AC16Y1_06275"
     CDS             complement(352..1059)
                     /locus_tag="AC16Y1_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIR5832100.1"
                     /translation="MTLTIIRLYHDLLGTYGDQGNAEVLIHRAHARQLQTELIEVTPG
                     MAIPRSGDIYLLGGGEDGPQTVSLELMAKDGGLTAAVNSGATVFAVCAGFQVIGTSLP
                     GPDGLIIDGLGLIDAQTVVNAAPRSVGELVVESTKNDLPILTGFENHQGLTNLGQDVT
                     PLGKVLTGIGNGFDRTEGVWQGRVIGTYMHGPALVRNPQLADAILESGTGAKLEKFDD
                     EFANALAAERRSSLLSK"
     gene            complement(1056..2252)
                     /locus_tag="AC16Y1_06280"
     CDS             complement(1056..2252)
                     /locus_tag="AC16Y1_06280"
                     /inference="COORDINATES: protein motif:HMM:NF019850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HIR5832101.1"
                     /translation="MAGNKSVRLAFANLAGQCAGTISKVLKRGQGETIKGRVILSLDK
                     DAIKKLAVNRDIVLVSGTNGKSTTTANLARILEVGTGEKVNTSETGANMAAGIAAVLA
                     QKPKHRYVVLECDELYLPTMYELLAPKVIVLLNLSRDQLHRTGEVRKVANLWHNTFTH
                     DDVTLIIDRDDPFLEFAAANAGHVIRVTFGGRRHPDAATCPNCAQILNWSLGDYRCSC
                     GLGSKLPQIRADKKLSGPARNSLLAIEAARLMGATVPIELQEELTANPPDRQEVFNAS
                     GRNIPTRLAKNPESWRESLSSLTSSNIILALNARGIDGRDTSWLWDIDFAALVGKKVF
                     CVGERKLDIAYRLHVQGIDVETADSFAMAAEQLPLGPVEAIASYTAFADLVAVQKGIH
                     TRGVGR"
     gene            complement(2261..2656)
                     /locus_tag="AC16Y1_06285"
     CDS             complement(2261..2656)
                     /locus_tag="AC16Y1_06285"
                     /inference="COORDINATES: protein motif:HMM:NF023691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HIR5832102.1"
                     /translation="MKFGGKLFIAFTIFYALVTVVYWYMTQEVIGTTVLALTGGLSFL
                     IGFYALFTSNRVGLLPEDIEYAKISDIDSDYGFFSPHSWTPLAIGFATFIFVLGFVFA
                     RWLMVLGTMAIIAAVAALLFEYYRGEFVK"
     gene            complement(2660..>4120)
                     /gene="ctaD"
                     /locus_tag="AC16Y1_06290"
     CDS             complement(2660..>4120)
                     /gene="ctaD"
                     /locus_tag="AC16Y1_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018849752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome oxidase subunit I"
                     /protein_id="HIR5832103.1"
                     /translation="MHGTLMLFLFATALFAGFANAIMPLQIGSPDVSFPRLNMISYWL
                     YFFGGMIVMLGFLTPGGAAGFGWFAYAPLSNVVNSPNIGSDLWIMGLTMSGMASILGA
                     VNFITTIFTMRAPGMTMFRMPLFTWNVLVTSILILMAFPVLAAAMLMLESDRKFGTQV
                     FAAENGGAILWQHLFWFFGHPEVYILALPFFGIASEILPVFSRKPIFGYKGLVYATIA
                     IAALSVSVWAHHMYVTGAVSLPFFAFMTMLIAVPTGVKFFNWIGTMWRGSVTFESPML
                     WIIGFLATFLFGGLTGILLAAPPIDFHVSDTYFVVAHFHYVLFGTVVFAMFAGFYFWW
                     PKFTGRMMDEKLAKLHFWLVFFGFHITFLVQHWLGAEGMPRRYSDYLASDGFTTLNIV
                     STIGSIVLGASTFVFFWNVYITYKRAPMVNQDDPWGWGTSLEWATSCPPPRHNFTSIP
                     PIRSERPAFDLHHVEHTDLSPEARQEIADFIAGKGR"
BASE COUNT         1208 a          990 c          867 g         1055 t
ORIGIN      
        1 ttaactttcg ttacctacct gtctttagcg tggggaatct ttattaatct agccgtggca
       61 tcaaatcaga aatttgccct tcctattgct gccggcggaa agtttgagaa ttttccaaaa
      121 gctgttcgaa ttgtttattt cattcaatct gtttggattt gctatcagac ctggattttt
      181 tatcaattaa tctctggaaa tacgatcaag ccatcctgga ttatttttat attcatttca
      241 cttgggctca taggaacgct gttaaaccta atttccagga gtaaattcga aaggctcaat
      301 gcaattcccc ttgcgctcat aacttatagt ttttggcttt tgcgctaagt tttacttact
      361 taataagcta ctacggcgtt cggctgctaa agcattagca aactcgtcat caaatttttc
      421 aagtttcgca cctgtgccac tttctaaaat tgcatcagct aattgtggat tacgaactaa
      481 agcgggtcca tgcatgtaag tacctattac tctgccttgc caaacgcctt cggtgcgatc
      541 gaatccatta ccaatgccag ttaagacttt gccaagtggt gtgacgtcct ggcccagatt
      601 ggtaagccct tgatgatttt caaaacctgt aaggatcgga aggtcattct tcgttgactc
      661 aaccaccaat tccccaacac ttctcggtgc tgcgttaact actgtttgag catcaattag
      721 tccaagcccg tcgattatca aaccatctgg tccgggcaaa gaggtgccga taacttgaaa
      781 tccagcacat accgcaaaaa ctgtggcacc tgagttcact gctgcagtta gcccgccatc
      841 cttagccatc agctccagac ttactgtttg tggtccatct tccccaccac caagtaaata
      901 aatgtcgccg cttcgtggaa tggccatccc gggagtcact tcaatcaact ctgtttgtaa
      961 ttggcgggca tgtgctcgat gaattaaaac ttcagcattt ccttgatcgc catatgttcc
     1021 aagcagatcg tgataaagcc gaatgatagt taacgtcatc ggcctactcc tcttgtgtgg
     1081 ataccttttt gcactgcaac taaatcagca aaggcggtat agctggcaat cgcctcaact
     1141 gggcctaatg gcagttgctc tgcagccatg gcaaagctgt cggcagtctc aacatctatc
     1201 ccttggacat gcagccgata agcaatatcc aatttgcgct cacctacaca gaagactttt
     1261 ttcccaacta aggcagcaaa atcaatatcc cacagccacg aagtatctcg cccatcaatt
     1321 cctcttgcat tgagcgccag gataatgttt gaacttgtta gcgaagataa actttcgcgc
     1381 cagctttcag gattttttgc caagcgggta ggaatgttgc gtcccgacgc attaaatact
     1441 tcttgtcgat caggtggatt tgcggtcaac tcttcttgta actcaatagg tacagttgcg
     1501 cccattaagc gggcagcctc gattgccaat aatgagttac gagctggacc agaaagtttt
     1561 ttatctgccc gaatctgagg caacttacta cctaatccac aactgcagcg atagtccccc
     1621 agcgaccagt tcaaaatttg cgcacaattc ggacaagtgg ctgcatccgg gtgacgtcgc
     1681 ccgccaaatg taactctgat gacatgaccg gcatttgccg cagcaaactc caagaacgga
     1741 tcatctcgat caattatcag agtgacatcg tcatgggtga acgtgttatg ccataaattt
     1801 gccacttttc gaacttcgcc cgtgcgatgt agttgatccc gcgaaagatt caaaagcaca
     1861 atcacttttg gcgcaagtaa ttcatacata gttggtaagt aaagctcatc gcactccagg
     1921 acaacataac gatgttttgg tttctgtgct aatactgcag ctattccggc tgccatgttt
     1981 gctccagttt cactggtatt tactttttca ccggtgccga cttctaaaat cctggccaag
     2041 tttgcggtag ttgttgattt accatttgtg ccactgacca gaacgatgtc acgatttact
     2101 gcaagctttt tgatcgcatc tttgtcaaga gacaaaatca cgcggccttt aattgtttcg
     2161 ccttggcctc gtttcaaaac ttttgaaatt gtgccagcac attgcccagc aaggtttgca
     2221 aatgctagcc gaacgctttt attaccggcc attggaaaaa ttacttaacg aattcaccgc
     2281 ggtagtactc gaatagcaag gcagccactg ctgcaattat tgccattgtg ccaagcacca
     2341 ttaaccagcg ggcaaagaca aatccgagta cgaaaataaa tgtggcaaaa ccaattgcca
     2401 gtggtgtcca agaatgtggc gagaagaagc cataatcaga gtcaatgtct gagatcttgg
     2461 cgtactcgat atcttctggc aataatccaa cacgattaga agtaaataat gcgtagaagc
     2521 cgattaggaa cgataggcca ccggttaagg caagcacagt tgtaccgata acttcttgag
     2581 tcatgtacca gtaaacaact gtgactaatg cgtagaaaat cgtaaacgca atgaaaagct
     2641 ttccgccaaa tttcatttgt tagcgacctt tcccagcaat aaaatcagca atttcttgtc
     2701 gagcctctgg gcttaaatca gtgtgctcaa catggtgtaa gtcaaaagct ggacgctcgc
     2761 tacgaatcgg tggaattgaa gtgaagttgt gccgtggtgg tggacaagag gtcgcccatt
     2821 caagggaagt tccccagccc catgggtcat cttgattaac cattggcgcc cgcttgtagg
     2881 tgatgtaaac attccagaaa aagacgaatg tggacgcacc caaaacaata gatccgatgg
     2941 ttgaaacaat atttaacgtg gtgaaaccat cactagctaa ataatctgaa taacgacgag
     3001 gcataccctc agcgcccaac cagtgttgga caaggaaagt tatgtggaag ccaaaaaata
     3061 ctaaccagaa gtgcaactta gctaactttt catccatcat gcggccagta aatttaggcc
     3121 accagaaata aaagccagca aacatcgcaa acacaacagt gccaaacagt acgtaatgga
     3181 agtgcgcaac tacgaagtag gtgtcactta cgtggaagtc aatgggtggt gcggccaaca
     3241 aaataccagt taagccaccg aataagaaag tagccaggaa gccaattatc cagagcattg
     3301 gcgattcaaa agtgaccgag ccccgccaca tcgttccaat ccagttgaag aatttcacgc
     3361 cggtcggcac agctatcaac atcgtcataa atgcaaagaa cggcaaactc accgcaccgg
     3421 ttacatacat gtggtgtgcc cagacagaga ctgaaagtgc agcaatcgcg atagttgcgt
     3481 agaccaagcc tttataacca aagattggct tgcgactaaa tactggcaaa atttcagatg
     3541 caataccaaa gaatggcaac gccaaaatgt aaacctcagg gtgaccgaag aaccagaata
     3601 aatgctgcca caagattgcc ccaccatttt cagcagcaaa aacttgagtg ccaaacttgc
     3661 gatcactttc aagcattaac atggccgcag ctagaactgg gaatgccatc aaaatcaaaa
     3721 tacttgtgac aagaacattc caggtgaata acggcattcg gaacatcgtc atacctggcg
     3781 cacgcattgt aaatattgtg gtgatgaagt taaccgcacc cagaatcgaa gccatgccag
     3841 acatagttag acccataatc cataggtcag aacctatgtt tggtgaatta actacatttg
     3901 aaagcggtgc ataagcaaac caaccaaaac cagccgctcc acctggggtt aaaaagccca
     3961 gcatcacgat catgccaccg aagaagtaca accaatagct gatcatgttt aaccgcggga
     4021 atgatacgtc tggtgagcca atttgtagcg gcatgattgc gtttgcgaaa ccagcaaata
     4081 atgcagtcgc aaataagaaa agcatcagtg tgccgtgcat
//
LOCUS       DBNHVN010000325         3794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_359988, whole genome shotgun sequence.
ACCESSION   DBNHVN010000325 DBNHVN010000000
VERSION     DBNHVN010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_359988"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="AC16Y1_06295"
     CDS             complement(<1..226)
                     /locus_tag="AC16Y1_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5832104.1"
                     /translation="MSKVLQNFINGQSVKSSSGKTTQLVDPSTGQGYAEAQLSNEKDV
                     ASAYEAAQTAFESWRETTPSERQKALLQIAD"
     gene            439..897
                     /locus_tag="AC16Y1_06300"
     CDS             439..897
                     /locus_tag="AC16Y1_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006602804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR5832105.1"
                     /translation="MSKVSPLDEVSKSIIEQLQEDGRRPYASIGKAVGLSEAAVRQRV
                     ARLLETGVMQIVAVTDPMQVGFNREAMIGIEVDGDIEAVANVIEKMPEVSYLVITAGS
                     FDILLEVVCDDDEALLEVINRKIRALPGVRRTETFMYLKLRKQIYTWGTR"
     gene            911..2293
                     /locus_tag="AC16Y1_06305"
     CDS             911..2293
                     /locus_tag="AC16Y1_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018190843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR5832106.1"
                     /translation="MTTLEQNTTNYSTSAKDNLWMHFTRHSGFEQGGEVPIIVRGEGA
                     RIFDDKGKSYIDGLAGLFTVQVGHGRKELAEAAAKQAEQLAFFPLWSYAHPKAIELAE
                     RLVSYAPSNLNRVFFTTGGGEAVESAWKAAKQYYKLIGKPMKHKVVSRAVAYHGTPQG
                     ALAITGIPDAKKFFEPLTPGGFRVPNTNFYRAEYHTDDIKAFGLWAANRIEEAILFEG
                     ADTVAAVFLEPVQNSGGCFPPPPGYLERVREICDQYDVLLVADETITGFGRIGSMFAM
                     NRFGVQPDILTMAKGMTSGYSPIGAMMVGDKVFEPFKQGMNYFAHGYTFGGHPVSAAV
                     ALANLDIFEREGLNQHVQENEGAFRRTLEKLNDLDIVGDVRGEGFFYGIELVKDKKTK
                     ETFSPDESERLLRGYLSKALFENGLYCRADDRGDPVIQLSPPLIVGQKEFDEMEQILR
                     AVLIEGMNVL"
     gene            2300..3682
                     /locus_tag="AC16Y1_06310"
     CDS             2300..3682
                     /locus_tag="AC16Y1_06310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013474084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5832107.1"
                     /translation="MSTNSNISFWWDTLSPSLTANVREQLAHDLEVDVAIVGAGYTGL
                     WTAYYLKSRKPDLEIAIIDANKVGFGASGRNGGWCSALFPTSMDKLASKFGVQSAVNM
                     QTAMHQTVDEVGRVIARENISCDWDKGGTLVFARSQLQKNQLQKEVLDWQNWGFTDSD
                     YRFLDQAETASRVNVSNNFGAFFTPHCAALNPAKLVRQLAQVVEHQGVKIYENTQATR
                     IDPGMVITDSGVICARYVVRATEGYTANIAGLKRQLAPIYSLMLATEPLSDQTWNEIG
                     LTNRETFSDGRNLIIYGQRTSDNRIAFGGRGAPYHFGSRIRPEYDQVKKVHHELEKIL
                     VELFPALAKTKITHTWGGPLGISRDWMPSATFDPHTGLAHAGGYVGDGVGSSNLAGRT
                     LADLITGTDSPLVKLPWVNHRWPNWEPEPFRWLGANLGLQAMTLADRTEQKSGKSSTI
                     AKWMGKLTGK"
BASE COUNT         1026 a          802 c          931 g         1035 t
ORIGIN      
        1 catcagctat ttgcagcagc gctttttgtc gctcggaagg tgtggtttcg cgccaagatt
       61 cgaatgcagt ctgagcagcc tcatatgcgc tcgccacatc tttttcattt gaaagttgag
      121 cttcggcata gccttggcca gtagatggat caactaactg cgttgttttc ccgctagagc
      181 tttttactga ttgaccattg atgaagttct gcagaacttt ggacacttgt cttcctccat
      241 tttgtgctga acgctttaat ccaggtattg cgttattcga actttacgtt gggtaacaag
      301 gttttggtag ggttttttta gtattttttt tgcggtttta gtaacaaatt ggttacaaac
      361 ctgctaaatc cgcagcaaaa ttgcgttttc actgcgaaat cacttgattg tccttcgaaa
      421 accattcatg atggaggtat gtcgaaagta agcccgctag atgaggtctc aaagtccatc
      481 atcgagcagc tacaagaaga tggtcgccgg ccgtatgcca gcattggcaa agccgttggc
      541 ctttccgaag cagctgttcg ccagcgggtt gccagacttc tggaaactgg cgtcatgcaa
      601 attgtggcgg taaccgaccc aatgcaagtt gggtttaacc gggaagccat gatcggcatc
      661 gaagttgatg gcgatattga agcagtggca aatgtaattg aaaaaatgcc tgaagtttct
      721 tacctcgtaa tcactgccgg tagtttcgac attctccttg aggttgtttg tgacgatgac
      781 gaggcgttac tcgaagtaat caaccgcaag atccgtgcgc tccctggggt ccgccgtacg
      841 gaaacattca tgtatctgaa attgcgcaag caaatttata catggggaac ccgttaaaca
      901 ggaaaaaacc atgacaacgc tagaacaaaa cacaactaac tactcaacat cagcaaaaga
      961 taatttatgg atgcacttca ctcggcattc gggctttgag caaggtggtg aagtaccaat
     1021 aatcgttcgt ggtgaaggtg cccgcatctt tgatgacaaa ggtaaaagct atatcgatgg
     1081 tttagctgga ttgttcaccg tccaagtggg acatggtcga aaagaattag ctgaagcagc
     1141 cgcaaagcag gccgagcaac tagcattctt cccgctctgg tcttatgcgc atccaaaggc
     1201 gattgaacta gccgagcgtt tagtttcata tgccccaagt aatttgaatc gagttttctt
     1261 taccactggt ggtggcgaag ctgttgaatc tgcatggaaa gctgccaagc agtactacaa
     1321 gttaattggt aaaccaatga aacataaagt ggttagtcgc gcagtggctt accacggcac
     1381 tccccaaggt gcattggcga tcactggtat tccagatgca aagaagtttt ttgagccact
     1441 caccccaggc ggtttccggg taccaaatac aaacttctat cgcgctgagt atcacacgga
     1501 tgacatcaag gccttcggac tttgggctgc aaatcgaatc gaagaagcga ttttgttcga
     1561 aggtgcagac acagtcgcag cagtatttct tgaaccagtg cagaactcgg gcggatgttt
     1621 cccgccaccg ccaggatatt tggagcgagt tcgagaaatc tgcgatcaat atgacgtact
     1681 gcttgttgcc gatgaaacca ttactggttt tgggcgaatt ggcagcatgt ttgcgatgaa
     1741 tcgctttggg gttcaaccag atattttgac gatggccaaa ggaatgactt ctggctactc
     1801 acctattggc gcaatgatgg ttggtgacaa agtattcgaa ccgtttaaac agggaatgaa
     1861 ctactttgca catggataca ccttcggtgg tcatccagta tctgctgcgg tagcgctagc
     1921 taatttagat atctttgaac gagaaggact taaccagcac gttcaagaaa atgaaggtgc
     1981 tttccgccgc actttggaaa aacttaatga cctggacatc gttggcgatg tccgcggtga
     2041 aggcttcttc tacgggattg agttagtcaa agataagaaa actaaagaaa ctttcagccc
     2101 tgatgagtca gagcgattac ttcgtggtta cttgtcaaag gctttattcg aaaatggttt
     2161 gtactgtcgc gctgatgatc gtggcgaccc ggtaatccag ttatcgccac cgctgattgt
     2221 tgggcaaaaa gagtttgatg aaatggaaca aattctgcgc gccgtactta tcgaaggcat
     2281 gaacgtactt taggtaaatg tgagtaccaa tagcaacatc tccttctggt gggatacgct
     2341 ttcgccttcg ttaaccgcga atgttagaga gcagttagcc cacgatttag aagttgatgt
     2401 tgctattgtc ggtgctggtt acaccggact ttggacggct tactacttaa aatcgcgcaa
     2461 accagattta gaaatcgcca taatcgatgc gaacaaagtt ggttttggtg catctggaag
     2521 aaatggtggc tggtgctcgg ccttattccc cacttcgatg gataaattag ccagcaagtt
     2581 tggagtccaa agtgcggtga acatgcaaac tgcaatgcac caaacagttg atgaagttgg
     2641 tcgggtaatt gcccgcgaaa atatttcctg cgattgggat aaaggcggca cacttgtttt
     2701 tgccaggtcc cagctacaaa aaaaccagtt gcaaaaagag gttttggatt ggcaaaactg
     2761 gggttttact gattcagatt atcgatttct agatcaggct gaaacggcaa gtcgagtaaa
     2821 tgtgtcaaat aattttggtg ctttctttac cccacattgt gctgcgttaa atcctgcaaa
     2881 gttagtcaga cagttagcac aggtagttga acatcaaggc gtaaaaattt acgaaaatac
     2941 tcaagccacc cgaattgatc ctgggatggt aattacagat tctggggtga tttgcgctcg
     3001 atatgtcgtt cgcgctactg aaggttacac tgcaaatatc gccggactaa aaaggcagct
     3061 agctcctatc tattcattga tgttggctac tgaaccgtta agtgatcaaa cgtggaatga
     3121 aattggcttg accaatcgtg aaacttttag tgacggccgg aatttaatta tttacggaca
     3181 acgaactagc gataatcgaa ttgcttttgg cgggcgcggt gcaccatacc attttgggtc
     3241 aagaataaga cctgaatatg accaggttaa aaaagttcac catgaattag aaaagatttt
     3301 ggtggaactt tttccagcgc tagcaaagac aaaaattact catacctggg gtggtccgct
     3361 tggtatttcg cgtgactgga tgccatcggc aacttttgat ccacataccg gcttagcgca
     3421 cgccggcgga tacgtcggcg atggcgtggg tagcagtaac ttagctggac gcaccttggc
     3481 agatttaatc accggtactg atagtcccct agtgaaacta ccttgggtta atcaccgctg
     3541 gccaaattgg gagcctgaac catttaggtg gttaggtgcg aaccttggct tgcaagcaat
     3601 gacattggct gatcgcacag aacaaaagtc tggcaaatct tctaccattg ctaaatggat
     3661 gggcaaactt actggaaaat gaaaatggct aaccgcagtt agccattttt gtttagtttg
     3721 aattaatttg cgtaggctgc aattccagtt agtgcttgac ctaccaccaa ggtgtggatc
     3781 tcatttgtac cttc
//
LOCUS       DBNHVN010000326         1434 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1029067, whole genome shotgun sequence.
ACCESSION   DBNHVN010000326 DBNHVN010000000
VERSION     DBNHVN010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1434)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1434)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1434
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1029067"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="AC16Y1_06315"
     CDS             <1..225
                     /locus_tag="AC16Y1_06315"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006944026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HIR5832108.1"
                     /translation="RRPQVQERLTTQIADAMVNILDARGVIVVIECEHLCMSMRGIRK
                     PGATTVTSAVRGQLRDATTRAEAMSLINGK"
     gene            226..1086
                     /gene="folP"
                     /locus_tag="AC16Y1_06320"
     CDS             226..1086
                     /gene="folP"
                     /locus_tag="AC16Y1_06320"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020490570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR5832109.1"
                     /translation="MLKPSGLPDSLAKLNRTLVMGVLNVTPDSFSDGGKFAATETAIV
                     HGKQMFTDGADVVDVGGESTRPGAERISVEEELSRVIPVIENLASNGIPVSIDTMRAE
                     VAKQAVAAGACIVNDVSGGKADPEMFQFLNTIELPYIMMHWRGHSTEMMNLTNYDDVV
                     TDVCDELQSQANAAVSAGIAISRLVLDPGIGFAKTTEQNWPLLGQLEILQDLGLPLLI
                     GASRKKFLGELLAKEGQPRELDGREAATTALTTLMAMQSVWAVRVHDAKSASDAVKVV
                     SRIMQEQGNG"
     gene            1079..>1434
                     /gene="folB"
                     /locus_tag="AC16Y1_06325"
     CDS             1079..>1434
                     /gene="folB"
                     /locus_tag="AC16Y1_06325"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002858274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR5832110.1"
                     /translation="MAEYQIELTGLTGFGYHGVLQSERENGQDFAVDVTLLVESPQVN
                     DDIKNTVNYAEVAKLVHQFVTGTPVDLIETLADNIADQLAKLPLVNAVEVTVNKPSAP
                     IDLPFENVSVTVYRGA"
BASE COUNT          427 a          291 c          342 g          374 t
ORIGIN      
        1 cgtcgcccgc aagtacaaga acgtttaacc acccaaattg cagatgcaat ggtaaatatt
       61 cttgatgccc gcggtgtgat cgttgttatt gaatgtgaac atttatgtat gtcgatgcgc
      121 ggaattcgta aacctggagc aactacagtt acttctgcag tgcgtggtca acttcgcgat
      181 gcgacaactc gcgctgaagc aatgtctctt atcaacggaa aataaatgct taagccaagc
      241 gggctacctg attcactagc caagttaaat cgcacattag ttatgggtgt gctgaatgta
      301 actcctgatt catttagcga tggtggaaaa tttgccgcaa cagaaactgc aatagtccac
      361 ggcaaacaaa tgttcacaga tggtgctgat gtcgtagatg ttggtggtga atcaacaagg
      421 cctggcgctg aacggatttc tgtcgaagaa gaattaagcc gagttattcc agttatagaa
      481 aatttagcca gtaacggtat tccagtaagt attgacacaa tgcgtgccga agttgcaaag
      541 caagctgttg ctgctggggc ttgcattgtt aatgatgtca gcggtggcaa agctgaccct
      601 gaaatgtttc aatttctaaa cacaatcgaa ttgccttaca tcatgatgca ttggcgcggt
      661 cactctacag aaatgatgaa tctaaccaac tatgacgatg tagtaacaga tgtgtgcgat
      721 gaattgcaat cgcaggcaaa tgcggcggta tctgctggaa ttgcaatctc gcgattggtt
      781 ttagatccag gaattggctt tgcaaaaacc acggaacaaa attggccgct gcttggtcaa
      841 ttagagattt tgcaagatct gggattacca cttctgattg gtgcgagtcg taaaaaattc
      901 ctcggtgaat tattagccaa agaaggtcaa ccacgtgaac tggatggtcg cgaagcagca
      961 accacagcgc taactacctt gatggcaatg caatcagttt gggccgtgcg agtacacgat
     1021 gcaaaatcag caagcgatgc agtaaaagta gttagccgaa taatgcagga gcaaggaaat
     1081 ggctgagtat caaattgaat taactggtct aactggtttt ggctatcacg gtgtattgca
     1141 atccgaacgt gaaaacggac aagatttcgc agtcgatgtg actttattag ttgagagtcc
     1201 gcaagttaat gacgacatca agaacaccgt taactacgca gaagttgcta agttagtcca
     1261 ccaattcgta actggaacac cagttgattt aattgagacc ttggccgaca acatcgccga
     1321 ccaattagct aaattgccac tagttaatgc cgtagaagta acggtcaata agccaagtgc
     1381 accaatcgat ttaccttttg aaaacgtttc tgtgactgtt taccgcggtg ctta
//
LOCUS       DBNHVN010000327         1021 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1253119, whole genome shotgun sequence.
ACCESSION   DBNHVN010000327 DBNHVN010000000
VERSION     DBNHVN010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1021
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1253119"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="AC16Y1_06330"
     CDS             <1..571
                     /locus_tag="AC16Y1_06330"
                     /inference="COORDINATES: protein motif:HMM:NF020752.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HIR5832111.1"
                     /translation="VVKRVRPIELRWYLASAHYRSNLEFSDSALTESATAFQRIEGFV
                     KRATAIVGDVDLTSTPICSDFEIAMNEDLNVPSALAALHEVVGEGNTLLANNASKDAI
                     KGCLASVRKMLDILGVDPLAQMWNQANADSAALTTALDQLIQDQIKLRLQAKANKDFA
                     TADQIRDELRAAGVLLEDTADGARWTLDQ"
     gene            571..>1021
                     /locus_tag="AC16Y1_06335"
     CDS             571..>1021
                     /locus_tag="AC16Y1_06335"
                     /inference="COORDINATES: protein motif:HMM:NF019644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR5832112.1"
                     /translation="MAGNSKRKGAIRTSKKGPLVGSGGRGKQKLEGRGPTPKASERPK
                     HPAAKRKRAAQKAEAKGGRVTTRSQEGKAKAIRPRDRDGIEWVAGKNSCLEALRADIP
                     ANALYLQQHLTVDDRVKEIIELANERGLALLESPRGELDRMTDGALHQ"
BASE COUNT          318 a          222 c          246 g          235 t
ORIGIN      
        1 agttgtaaaa cgggtgcggc caattgaact tcgctggtat ttagctagcg cgcattaccg
       61 cagcaacttg gaattttcag atagcgcatt aactgaatca gcaactgcat tccaacgcat
      121 cgaaggattt gttaaacggg caacggcaat tgttggtgac gttgatttaa ccagcacacc
      181 aatttgttca gactttgaaa ttgcgatgaa tgaagatcta aatgtgccat ccgcgttagc
      241 tgcactgcat gaagtagttg gtgaaggtaa tactttgctt gctaacaatg ccagtaagga
      301 tgcaatcaaa ggttgcttag cctcagttcg aaaaatgctg gacatacttg gcgttgatcc
      361 acttgcacaa atgtggaatc aagcaaatgc agatagcgcc gcattaacca ctgctcttga
      421 tcaattaatt caagatcaaa ttaaattgcg cctccaagca aaggcaaata aagattttgc
      481 aacagctgat caaattcgag acgaacttcg tgcagctggc gtgctactgg aagacacagc
      541 tgatggtgca cgctggactt tggatcagta atggctggta attcaaaacg caaaggcgcg
      601 attcgcactt caaaaaaagg tccactagtt ggctctggtg ggcgcggtaa acaaaaactt
      661 gaaggccgcg gtccaacacc taaagcaagt gaacgtccaa agcaccctgc cgcaaaacgt
      721 aaacgcgctg cgcaaaaagc cgaagcaaaa ggtggtcgag ttaccacccg cagtcaagaa
      781 ggcaaagcta aagcaattcg cccgcgtgat cgtgatggaa tcgaatgggt agccggtaaa
      841 aatagttgtt tagaggcatt gcgagctgac atcccagcaa acgcacttta cttacaacaa
      901 cacttaacag tcgatgaccg agttaaagaa attattgaac tggcaaatga acgcggcttg
      961 gcgttacttg aatcaccacg tggtgaatta gatcggatga ctgatggtgc attgcaccaa
     1021 g
//
LOCUS       DBNHVN010000328         7867 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_955190, whole genome shotgun sequence.
ACCESSION   DBNHVN010000328 DBNHVN010000000
VERSION     DBNHVN010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 7867)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7867)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7867
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_955190"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="AC16Y1_06340"
     CDS             complement(<1..688)
                     /locus_tag="AC16Y1_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018509732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5832113.1"
                     /translation="MTKPIVLIAEELSPATLSVLGDGFEIKHCNGADRAELMQAIADV
                     DAILVRSATKVDAQAIAAAKNLKIIARAGVGLDNVDIPTATAAGVLVVNAPTSNIVSA
                     AELAVGLLLASARNIVPANLALKNGKWARSKYSGSELQDKTVGIIGMGKIGLLVAKRL
                     AGFDMKFVAYDPYVKQAPKGGPEIKMVEFDELLAVSDFITIHIPKTAETVGLIGEEAL
                     AKVKPSVHIIN"
     gene            complement(789..1421)
                     /locus_tag="AC16Y1_06345"
     CDS             complement(789..1421)
                     /locus_tag="AC16Y1_06345"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR5832114.1"
                     /translation="MSAKLKSIKSIQAYLATQDKKLGKWLIDAPLYPVAKEPAHNSHF
                     ETIVFAVIGQQLSGKAADTITHRLIAQTGSPVKPAKVLKLGHEKLREVGLSNAKARTI
                     LELSQAVKDGFALASVDELSDEEILKELTQFWGIGRWTVEMFMMFRLGRLDIWPAGDL
                     GVRKGWAKIQGLKVTPTEKEIVGMADHLRPYRSVVAWHSWRMLDEEPEFW"
     gene            complement(1421..2464)
                     /locus_tag="AC16Y1_06350"
     CDS             complement(1421..2464)
                     /locus_tag="AC16Y1_06350"
                     /EC_number="4.2.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0050346 - trans-L-3-hydroxyproline
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-3-hydroxy-L-proline dehydratase"
                     /protein_id="HIR5832115.1"
                     /translation="MRSSKVIHVVSVHAEGEVGDVIVGGVNPPPGETIWEQSRFIAND
                     QELRNFVLREPRGGVFRHVNLLVPPKNPKAAVGWIIMEPEDTPPMSGSNSMCVATVVL
                     ETGIVPMHEPETRFTMEAPGGLVEVVAQCKDGKATEIRVHNVPSFVDRLDVNIEVPGI
                     GTVKADIAYGGDSFAIVNAHELGFAIKPDEARELAEVGITITNAANEQIGFNHPTNSE
                     WKHISFCQIAAPVFEEDGVLISHNAVAIQPGKIDRSPTGTGVSARMAVLHHRGVLKVG
                     DQLRGRSIIGSHFNGRVEKEVSIGDRNGIIPSIAGQAWLTGTHQHTLDPSDPWPQGYV
                     LSDTWPQIKPKTS"
     gene            complement(2494..3567)
                     /locus_tag="AC16Y1_06355"
     CDS             complement(2494..3567)
                     /locus_tag="AC16Y1_06355"
                     /inference="COORDINATES: protein motif:HMM:NF024911.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5832116.1"
                     /translation="MPSALVTGTFNPPHMGHVKLIKAAIEKRDHVVVVVLAQPHDRVS
                     AADRAKALQQDCIAEGVAESNMTIVQGYESTPYDLDNAAAWDSNARVIQAHLRQTPPV
                     DLLVTSEKSGEKFAEKLGLHHFSFDPDRKLIPVSSTQIRKDLIGNWHLLGPGSRELLA
                     IRAVVIGAESTGTTTTTLAVQEALKKRGGNFSATNWIREYGRDLTMRKKEQAEAMGLA
                     DHAVPWTSTDFVEIAVVQQQLEDVAARTGGPVVCCDTDVFATIIWERRYLGNKANLQM
                     PNTTQNRIYFVTQPDGVPFVQDKIRDSENLRYSMTREFEDDLISTGRAWLSLDGNVET
                     RVELALDAIDSAMRQAFDFKTAE"
     gene            complement(3652..4680)
                     /gene="ilvC"
                     /locus_tag="AC16Y1_06360"
     CDS             complement(3652..4680)
                     /gene="ilvC"
                     /locus_tag="AC16Y1_06360"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR5832117.1"
                     /translation="MAELFYEDDADLAIIKKRKVAVIGYGSQGHAHALSLRDSGVDVR
                     VGLAEGSKSREKAQAEGLRVVDPATAVKEADLIMILVPDHVQRKVYAQAIEPNLNPGD
                     ALFFAHGFNIRFGYIKPPANVDVCMVAPKGPGHLVRREYVAGRGVPAIVAVEQDATGN
                     AWPLALSYAAAIGALRAGGIKTTFTEETETDLFGEQAVLCGGASALVQAGFETLVEAG
                     YQPEVAYFECLHELKLIVDLMYEGGIAKQRWSVSDTAEYGDYVSGPRVIDARVKESMK
                     SILKDIQSGSFAKRFIDDQDAGAPEFKALRAKGEAHPIEATGRELRKLMSWVKSSDSD
                     YKEGTAAR"
     gene            complement(4736..5260)
                     /gene="ilvN"
                     /locus_tag="AC16Y1_06365"
     CDS             complement(4736..5260)
                     /gene="ilvN"
                     /locus_tag="AC16Y1_06365"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018087233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR5832118.1"
                     /translation="MSKHTLSVLVQDQPGVLARISALFSRRGYNIESLAVGVTETPGI
                     SRMTIVVEVDEIALEQITKQLNKLINVLKIIELTDETTISRELMLIKVRADANARAAV
                     LQLVELFRAKVVDVGTDAVTIEATGSREKLEALLEVLAQHGIKEIVQSGVVALSRGGR
                     GIGDRALRSADKQA"
     gene            complement(5260..7008)
                     /locus_tag="AC16Y1_06370"
     CDS             complement(5260..7008)
                     /locus_tag="AC16Y1_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HIR5832119.1"
                     /translation="MAEVISGAQSLIRSLELSNVDVVFGIPGGAILPAYDPLMDSKSV
                     RHILVRHEQGAGHAAEGYAAATGRVGVCMATSGPGATNLVTPIADAHMDSVPMVAITG
                     QVGSASIGTDAFQEADIRGITMPITKHNYLVTRAEDIPGAMKEAFHLASSGRPGPVLV
                     DITKDALQNKAEFNWPTSIDLPGYKPTTKPHGRQIKEAARMIVEAHRPVLYVGGGIIR
                     SRASIELRKLAELTGIPVVTTLMARGAFPDSHPQHMGMPGMHGSVGAVGALQQSDLLI
                     TLGARFDDRVTGKLETFAPRAKVIHIDIDPAEIGKNRTADIPIVADLREALLQLTPAI
                     IAEFDAGNRADLTGWWRTLNHWRETYPLGYDLPDNGTLSPQLVIERLGKIAGPDAIYS
                     SGVGQHQMWAAQFIGYENPNTWLNSGGLGTMGYAIPAAMGAKVGMPDSTVWAIDGDGC
                     FQMTNQELATCTINNIPIKVALINNSSLGMVRQWQTLFYSERYSNTDLHSHSFPDFVK
                     LADAYGCLGLRCDSPEDVDATIEKAMGTNDVPVVVDFRVHRDAMVWPMVAAGTSNDEI
                     KFARDLAPKWERGDDE"
     gene            7367..>7867
                     /locus_tag="AC16Y1_06375"
     CDS             7367..>7867
                     /locus_tag="AC16Y1_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019126882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR5832120.1"
                     /translation="MTQYVYDFSEGDKDQKDLLGGKGANLAEMTRLGLPVPPGFTITT
                     EACREYLVGGTEPGPLRVQVTMALRRLEDQLGKRLGDVDDPLLVSVRSGAKFSMPGMM
                     ETVLNVGLNDYSVNGLTKASNNERFAWDSYRRLIQMFGKTVLDIEGEIFASALQRAKE
                     RNGVSSD"
BASE COUNT         2089 a         1915 c         1673 g         2190 t
ORIGIN      
        1 cattaataat gtgcacgcta ggtttcactt tggccaacgc ttcttcacca attaagccga
       61 cagtttcggc tgtctttgga atgtgtattg tgatgaaatc tgataccgca agcaactcat
      121 caaactcgac catctttatt tcagggccac ctttaggcgc ttgctttaca taaggatcgt
      181 aagcaacaaa tttcatatcg aatccagcaa gacgcttggc aactagtagc ccgattttgc
      241 ccatgccgat aatgccaaca gttttatctt gtaattcgct accactgtat ttagacctag
      301 cccacttacc gtttttgagc gctaagttag ccggaacaat atttcgagca ctagcgagca
      361 atagaccaac agccaactca gccgccgaaa ctatgttgct ggtcggcgca ttgactacaa
      421 gcactccagc cgcagtggcc gtggggatgt caacgttatc taagccaacg cctgcccggg
      481 caataatctt caaattttta gccgcggcga ttgcctgcgc atcaactttg gtggcggacc
      541 taaccagaat tgcatcaaca tctgcgattg cctgcattaa ctcagctcga tcagccccat
      601 tgcaatgttt gatttcaaat ccatcaccca aaacagaaag ggttgctggc gataattctt
      661 cagcaataag aacaattggc ttagtcatga cacgagttta gtttgaacag cttgatcaag
      721 gtttgcaagg aatttgcgat taccagaact caggttcttc agtacttagg gaagaacctg
      781 agttctggtc accagaactc aggttcttca tccaacatcc gccagctatg ccaagccacc
      841 acgctgcgat atggcctcaa gtgatctgcc atacctacta tttctttttc cgttggcgtc
      901 actttaagtc cttgaatttt ggcccaacct ttgcgcaccc ctaaatctcc agcaggccaa
      961 atgtctaacc gacccaaccg gaacatcata aacatttcaa cagtccaacg accaataccc
     1021 cagaactgag ttaattcttt aaggatctct tcatcgctta attcatcaac acttgctaag
     1081 gcaaagccgt ccttaacagc ctgactaagt tccaaaatag ttcgcgcttt tgcattactt
     1141 aaaccaactt cgcgtaattt ttcatgacct agttttaaaa ctttcgctgg tttgactggt
     1201 gatccagttt gagcaatcaa tctatgtgtg attgtgtcgg ctgcttttcc ggataactgt
     1261 tggccaatta cggcaaaaac gattgtttcg aagtgtgaat tatgcgctgg ttctttagca
     1321 actggataca gcggcgcatc aattaaccat ttacctaact tcttatcctg tgttgctaaa
     1381 taagcctgga tggatttaat cgactttaat tttgcagaca ttaactagtt ttaggtttaa
     1441 tttgtggcca ggtatcactc aacacataac cttgcggcca cggatcagat ggatcaagag
     1501 tgtgttggtg ggtaccggtt aaccaggcct ggcctgcaat tgaaggaatg atgccattcc
     1561 tatcgccaat cgagacttct ttttcaactc ggccgttgaa gtgcgagccg attattgaac
     1621 gaccacgtag ttggtcgcct acttttaata cgccgcggtg atgtagcact gccattcggg
     1681 cacttacacc ggtaccggta ggtgagcgat caatttttcc aggttgaatt gccactgcgt
     1741 tgtgacttat taacacacca tcctcttcaa aaactggagc agctatttgg caaaacgata
     1801 tgtgtttcca ttccgaattt gttggatggt taaacccaat ttgctcgttc gctgcattgg
     1861 taattgttat tccaacttca gctaactcac gagcctcatc tggttttatg gcaaagccca
     1921 actcatgcgc atttacgatc gcaaaactgt ccccgccata ggcaatatct gctttaacgg
     1981 ttccaattcc aggaacttca atatttacat caaggcgatc aacaaaagat ggcacattat
     2041 gaactcgtat ctcggtggct tttccgtcct tgcattgagc cactacttca actaagccac
     2101 ctggtgcttc catcgtgaat cgtgtttctg gttcgtgcat tggaacaatt ccggtttcca
     2161 aaaccacagt tgccacacac attgaattgg aaccagacat gggtggagtg tcttcaggct
     2221 ccataatgat ccagccaact gcggcctttg gattctttgg tggtaccaat aaattaacgt
     2281 gacgaaatac cccaccacgg ggttcacgca aaacaaaatt tctcagttct tgatcattag
     2341 caatgaatct tgattgttcc caaatagttt caccaggtgg tggatttacg ccacccacaa
     2401 ttacatcgcc aacttcacct tcagcatgca cgctaactac gtgaattact tttgaacttc
     2461 gcatgttcta actataaagt aagaatcttt ttcttattca gcagttttaa agtcaaatgc
     2521 ttgacgcatt gctgaatcaa ttgcatcgag cgccaactca actcgagttt caacgtttcc
     2581 atcaagtgaa agccaagcgc gacctgtgct aattaggtca tcttcaaatt ctcgtgtcat
     2641 tgagtaccgt aaattttcgc tatctcggat tttgtcttga acaaacggaa ccccgtcagg
     2701 ctgtgtcaca aagtaaattc ggttttgggt cgtatttggc atttgcagat ttgccttatt
     2761 gcccaagtat cgacgttccc aaataattgt ggcaaaaaca tcggtatcgc agcagacgac
     2821 tgggccacca gtacgagctg ccacatcctc taactgttgt tgcaccacag cgatttcaac
     2881 aaagtcagtt gaagtccaag gcactgcgtg gtcagctaag cccattgctt cagcttgttc
     2941 ttttttgcgc atggttaaat cacgaccgta ttcgcgaatc cagttagtgg ccgagaaatt
     3001 tccaccgcgc tttttcaatg cctcttggac ggcaagcgtc gttgtcgtcg tgccagtcga
     3061 ttcagcacca atgacaactg ctctgatcgc aagcaattca cgtgaaccag gtccgagtag
     3121 gtgccaattt ccaattaagt ctttgcgaat ttgcgttgaa gaaacaggaa tcaattttcg
     3181 atcagggtca aagctgaaat gatgtagccc aagtttttca gcaaacttct cgccagattt
     3241 ttcggaagta actagtaagt caactggtgg tgtctggcgc agatgcgctt gaataactcg
     3301 agcgtttgag tcccaggctg ccgcattatc taagtcgtat ggagttgact catagccctg
     3361 tactatcgtc atatttgatt cggcaacgcc ctcagcgatg caatcttgct gtaaagcttt
     3421 agctcgatca gcagcactaa ctcgatcatg tggctgagca agaaccacaa ctacaacgtg
     3481 atctcgtttt tcgattgcag ctttaatcag cttcacatga cccatatgag gtgggttaaa
     3541 tgtgccggtc actaatgcag aaggcatatg ttgagcctac cttttgctaa gcagaagtaa
     3601 aaataaaaaa agtccgccca acattagggc ggacttttta ttcaatgcga attagcgtgc
     3661 agccgtacct tctttgtagt cgctatcaga agacttaacc cacgacatta atttacgtaa
     3721 ttcacggcca gttgcttcaa ttggatgtgc ttcaccttta gcgcgcaatg ccttgaattc
     3781 tggagcaccg gcatcttgat catcaataaa gcgtttagca aatgaaccac tttgaatgtc
     3841 tttgagaatc gatttcattg actctttcac tcgagcatca attacgcgtg ggcctgagac
     3901 gtagtcaccg tattctgcag tatcagaaac tgaccaacgc tgttttgcga taccgccttc
     3961 gtacattagg tccacaatta acttaagttc atgtaaacac tcgaagtaag caacttctgg
     4021 ttggtaacca gcctcaacta gagtttcaaa tccagcttga actaaggcag atgcgccacc
     4081 gcaaagtact gcctgttcgc caaataagtc tgtttcggtt tcttcagtaa atgtagtttt
     4141 aattccacct gctcgtaatg caccaattgc agccgcataa gagagtgcaa gtggccaagc
     4201 attaccagtt gcatcttgtt cgactgcaac aatggctggc acgcctcggc cagcaacata
     4261 ttcgcggcga actaagtggc ctggaccttt gggcgcaacc atacaaacat ctacgtttgc
     4321 aggtggcttg atgtaaccaa accggatgtt aaatccatgt gcaaaaaata gtgcgtcacc
     4381 aggatttaaa tttggctcga ttgcttgagc ataaacttta cgttgcacat gatctggaac
     4441 cagaatcatg attaggtctg cttctttaac tgcagttgct ggatcaacaa cgcgtaaacc
     4501 ttcagcttgt gctttctctc gactctttga accttcagct aaaccaacgc ggacatctac
     4561 accactgtcg cgaaggctaa gtgcgtgtgc gtgaccttgg ctaccgtaac cgataaccgc
     4621 aaccttgcgc tttttaataa tggctagatc tgcgtcatct tcataaaata actcggccac
     4681 agtttctcct tatttagtgt gtttagtgtg ccagaaaaat taaaaattcc cgaatttatg
     4741 cttgcttatc ggcactgcgc agtgcgcgat cgccgattcc gcggccacct cgacttaatg
     4801 cgacaacgcc ggactgcaca atttccttaa tcccatgttg ggcaaggact tcgagcaggg
     4861 cttcaagttt ttcgcgagat ccagttgctt caatagtgac cgcatctgtg ccaacgtcaa
     4921 caaccttggc tcgaaacagt tcaactaact gcaaaacagc cgcccgcgca ttcgcatcgg
     4981 ctctaacttt tattaacatc agttcgcgcg aaattgtggt ctcatctgta agttcaataa
     5041 tctttagcac attaatcagt ttgtttaatt gcttggtgat ctgctcaagg gcaatttcat
     5101 ctacttcaac cacaatagtc atccgagaaa tacctggggt ctcagtcaca cctacagcta
     5161 aggattcaat gttgtaccca cgtcgagaga acaatgcaga aatacgagca agtacacctg
     5221 gttggtcttg cactagtaca gaaagagtgt gtttagacat tattcgtcat cccctctttc
     5281 ccacttcggt gccagatcgc gagcaaattt gatttcatcg ttactggttc cagctgcgac
     5341 catcggccaa accattgcat ctcgatgtac tcggaaatca acaactactg gaacatcatt
     5401 ggtgcccata gctttttcga ttgttgcatc aacatcttca ggagaatcac aacgtaagcc
     5461 caggcatccg taagcgtccg ctaacttcac aaaatcaggg aacgaatgag aatgtaaatc
     5521 agtgttgcta taacgttcgc tatagaacag ggtttgccat tggcgaacca tacccaatga
     5581 tgaattgtta attaacgcga ctttgattgg aatgttattg attgtgcaag tggccaactc
     5641 ttggttagtc atttggaagc atccgtcacc atcaatcgcc caaacagttg aatctggcat
     5701 accaactttg gctcccattg ctgccggaat tgcgtagccc atggtgccta atccaccaga
     5761 gttcaaccaa gtgtttggat tttcatagcc aataaattgg gcagcccaca tttgatgttg
     5821 tccaacgccc gatgagtaaa ttgcatctgg gccagcgatt tttcctaacc gctcaatcac
     5881 taactgcgga gaaagtgttc cattatccgg caagtcataa cctagcggat atgtttcacg
     5941 ccagtgatta agtgttcgcc accaacccgt taaatccgcg cgatttccag catcaaattc
     6001 cgcaataatt gctggagtta attgaagtaa tgcttctctt aagtcagcca caatcgggat
     6061 gtctgcagta cggttcttgc caatttcagc aggatctata tcaatgtgaa tcactttggc
     6121 gcgtggcgca aaagtttcta atttgccagt tactcgatcg tcaaaacgtg cgccaagcgt
     6181 aattagtaaa tcactttgtt gaagtgcgcc aactgcacct actgaaccat gcatgcccgg
     6241 catacccatg tgctgcggat ggctatctgg gaaagcgccg cgggccatca aagttgtaac
     6301 aacaggaatt ccagttaatt cagcgagttt gcgaagttca attgatgcac gtgaacggat
     6361 aattccgcca ccaacgtata gaactggacg atgagcctca acgatcatgc gcgcagcttc
     6421 tttaatctgt cgcccatgtg gtttcgtagt gggtttgtaa cctggtaaat caattgaagt
     6481 tggccaatta aattcggctt tgttttgcaa tgcatctttt gtgatatcaa ctaggactgg
     6541 tcctggccta ccactacttg ctaagtggaa tgcttctttc atcgcgcccg ggatatcttc
     6601 tgcgcgagta actaaataat tatgttttgt tattggcatt gtgatgccac ggatgtcagc
     6661 ctcttgaaat gcgtcggtac caattgaagc cgaaccaact tgaccagtga tcgccaccat
     6721 cggaactgag tccatatgtg catcagcaat tggcgtaact aagttagttg cacctggtcc
     6781 gcttgtcgcc atacagacac caacgcgacc agttgctgct gcataacctt cagcagcgtg
     6841 accggcacct tgctcgtggc gcaccaaaat atggcgaaca gactttgagt ccatcaaagg
     6901 gtcataggcg ggaagaattg cgccgccggg aataccgaaa actacatcga catttgaaag
     6961 ttcaagagag cgaataagac tttgtgcacc cgagataact tcagccacgg attgctccta
     7021 gttctaaaag aaggatcact tctttttaat ttgatccacc cagtagttgg ctttgacgat
     7081 atgctctctg cgccgcaagc gacttgtaac agaaagttgc gcccggattg ttggtcacgc
     7141 cccgattgca ccgtcaccac ctggtggtgt tagtggttct actctaagca aaaaagtcac
     7201 atcagtcaca tcggcaactt tttgtctcac taggtggact tagttggatc agcaggtagc
     7261 aggattctag tcagataaat caccgataaa tgcggatgaa aacgttacat tttgccaata
     7321 cgttaggggt gagagcaggt gctctatcta gaacggcagg actttcatga ctcagtacgt
     7381 ctacgacttc tctgaaggcg acaaagatca aaaagatctt ctaggtggca aaggcgccaa
     7441 ccttgctgag atgactcgac tgggcttacc ggttcctcct ggcttcacaa ttacgaccga
     7501 agcttgccga gaatacttag ttggtggcac cgaacctgga ccacttcgcg tgcaagtaac
     7561 gatggcactt cgtcgactag aagatcagct aggtaaacga cttggtgatg ttgatgatcc
     7621 actcttagtg agtgtgcgct caggtgcaaa attttccatg cccggaatga tggaaaccgt
     7681 tctaaacgtt ggcctcaatg attactcggt caatggtcta acaaaggcta gtaacaacga
     7741 acgttttgct tgggattcat accgacgact tatccaaatg tttggtaaaa ccgttcttga
     7801 tatagaaggt gaaattttcg ccagtgcatt acaacgagcc aaagagcgta atggagtttc
     7861 ctcggat
//
LOCUS       DBNHVN010000329         1447 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1549407, whole genome shotgun sequence.
ACCESSION   DBNHVN010000329 DBNHVN010000000
VERSION     DBNHVN010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1549407"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="AC16Y1_06380"
     CDS             complement(<1..255)
                     /locus_tag="AC16Y1_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018350100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HIR5832121.1"
                     /translation="MTRAVLVLEDGQVFTGNSYGAIGSAFGEAVFATGMTGYLETITD
                     PSYCGQVVIQTAPHIGNTGWNDEDDESTIIWVSGYVVRDPA"
     gene            complement(252..836)
                     /locus_tag="AC16Y1_06385"
     CDS             complement(252..836)
                     /locus_tag="AC16Y1_06385"
                     /inference="COORDINATES: protein motif:HMM:NF051760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH-like domain-containing protein"
                     /protein_id="HIR5832122.1"
                     /translation="MMFNTGLLKLAQSAPVTNVPLRTVLTLIVVAAILLSVLGMARGW
                     RKNLRTDLAQIDTQVPTGANQISPKVAARFAGTTTAGNWLDRITNQELGTPRGVDLQI
                     FDQGIFITDDTNFQLWIAKSQISKVATGQGIAGDVVEKSGMLIFTWRLGDTHLDTGVR
                     VSRHSDHELIVLALANFPGSDLSQSTNFDRGAGA"
     gene            complement(836..>1447)
                     /locus_tag="AC16Y1_06390"
     CDS             complement(836..>1447)
                     /locus_tag="AC16Y1_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009398028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5832123.1"
                     /translation="HVCHLSTAGSVEIIRWAKSRGINVTAEVTPHHLFFNDEVVSSYD
                     PIFKVNPPLRSAKDVQAVRDGLADGTIDIVATDHAPHPAEDKDCEWSAAAFGMTGLES
                     ALAIVNSTMVQTGKLSWRRVAEVMSINPAKIGRVLDQGQPLEVGSPANIVLFNPSVEL
                     KVDPTKSESGSRNTPYRELTLPGQVIATFFHGQPTVLAGQVVS"
BASE COUNT          373 a          364 c          317 g          393 t
ORIGIN      
        1 agctggatct cgaactacat aaccagaaac ccaaattatg gttgactcat catcttcgtc
       61 attccaccct gtgttaccaa tgtgcggtgc agtttgaata actacttgac cgcaataaga
      121 agggtcagta atagtttcta gataaccagt cattccagtt gcgaaaactg cttcgccaaa
      181 cgcgctacca atggcaccat aagaatttcc agtgaagact tgtccgtctt cgagcactaa
      241 tacggctcgt gtcacgcccc tgctcccctg tcgaaatttg tactctgcga aagatcagag
      301 ccggggaagt ttgccagtgc caaaactatt aattcatgat ccgagtgtcg gcttacccgc
      361 accccagtgt ccaggtgcgt atcgcccaat cgccaagtaa aaattagcat gccggatttt
      421 tcgactacat cgccggcaat tccttgacca gttgctactt tgctaatttg cgattttgcg
      481 atccacaact gaaagttggt gtcatcggtg ataaaaatac cttgatcaaa aatctgtaag
      541 tccacaccac gcggggtacc aagttcttga ttggttatcc ggtctaacca atttcctgca
      601 gttgtagttc cggcaaacct agccgcaact tttggtgata tctggttggc gccggttgga
      661 acttgggtgt cgatttgggc caagtcagtg cgcaagttct tgcgccaacc gcgggccatg
      721 ccaagcactg aaagtaagat tgccgccacc acgatcaaag taagtaccgt gcgaagtggc
      781 acattagtta ctggtgcaga ttgggccaat ttaagtaaac cggtattaaa catcattacg
      841 acactacctg cccagctaaa actgttggct ggccgtggaa gaaagtagca attacctgac
      901 caggcaaagt taattcgcgg tagggcgtat ttcgacttcc actttcgctc ttggttgggt
      961 ctactttcaa ttcaactgac ggattgaata acacaatgtt ggctggactg cccacttcaa
     1021 gtggttgacc ttgatctaac acccggccaa tttttgctgg gttaatcgac atcacttcag
     1081 ccacccgacg ccaagataat ttgccagttt gcaccatagt tgaattcaca attgctaacg
     1141 cagactctaa accagtcatt ccaaatgctg ccgctgacca ttcgcagtct ttgtcttctg
     1201 ctggatgtgg tgcgtgatcg gtggcaacta tgtcgatagt tccatctgcc aaaccgtccc
     1261 gcaccgcttg aacatctttt gcactccgaa gtggtggatt tactttaaat atcggatcgt
     1321 atgaactaac tacttcgtca ttaaaaaata agtggtgcgg agtaacttct gcagtgacat
     1381 ttatcccgcg acttttggcc cacctgatta tttccacgct gccagcagtt gatagatgac
     1441 aaacatg
//
LOCUS       DBNHVN010000330         1272 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1922078, whole genome shotgun sequence.
ACCESSION   DBNHVN010000330 DBNHVN010000000
VERSION     DBNHVN010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1922078"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..>1272)
                     /locus_tag="AC16Y1_06395"
     CDS             complement(173..>1272)
                     /locus_tag="AC16Y1_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011731466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR5832124.1"
                     /translation="PYGVAILRYEDANALQNDKRLHQGTRRWPEHNGIESDLLVPWWN
                     EIMLSLEGEDHIRIRKLANPAFSPRIIETLTPKFAKLANDLIDDFCEKGSCEFMSEFA
                     EPYSAKVITLLLGLPENLWPEIADLATKLGYVFSVTFKEDLPEIEEGLSGILRITQQL
                     IDTHQNLDSDSFISTMVQASVDGQKITERELLVMLSFIIFAGFDTTRNQLGLGMQTFT
                     KNKEQWALLGQNPEHARIAVEEVMRLNPTITWFTREAVEDFDYKDLHIEKGMTLQFYN
                     VQIGTDHRKYEPLEMDITASRAPHFGFGGGMHHCLGHYVARIDMQEALKALSKRLPDF
                     QIEHGAHYLPDSGNTGPIELPISFTPSERYS"
BASE COUNT          369 a          279 c          249 g          375 t
ORIGIN      
        1 taattccaat ggcaccaaca aaaccaaatc gcccaaggga aacgttaaaa cctgttagta
       61 acagaattaa ttctgagggg attggcagga ttacaacttc aattgcaatt aataaacccg
      121 caccaagtaa tccaatttga tcaattagat ttaccgcaaa ttcaagcact tgctaagaat
      181 accgttcact tggcgtgaaa ctaattggca actcgatcgg accggtgttt ccactatcag
      241 gtaagtagtg agcgccatgt tcaatctgaa aatctggcaa ccgcttagaa agtgccttta
      301 atgcttcttg catatcgatc cgggcaacat aatggcctag acaatgatgc attccgccac
      361 caaatccaaa atgtggtgct cgcgatgccg ttatgtccat ttccagcggt tcgtattttc
      421 tgtgatcggt gccgatttga acgttataaa actgcagcgt catgcctttt tctatatgta
      481 aatctttgta atcaaaatct tcgacagcct cacgtgtaaa ccaagtgata gttggattta
      541 gtcgcataac ttcttcgact gctattcgag catgttctgg attttggcca agaagcgccc
      601 attgctcttt attttttgta aacgtttgca tacccagacc aagttgatta cgcgtcgtat
      661 caaatcccgc aaaaataata aacgagagca ttactaaaag ttcgcgctca gttatttttt
      721 ggccatcaac actggcttga accatggtgg aaataaaact gtcactatcc aggttttgat
      781 gggtgtcgat cagctgctga gtaattcgaa gtatcccgct taatccttct tcaatttcag
      841 gaagatcctc tttaaatgtc acgctaaaca catagccaag tttggtagct aaatccgcaa
      901 tctctggcca caaattttct ggaagcccca gtaaaagtgt aattaccttg gccgaatagg
      961 gctcggcaaa ttcactcatg aattcgcaag atcctttttc gcaaaagtca tcaatcaaat
     1021 cgtttgcaag ttttgcaaat tttggagtta gtgtctcaat gattcgagga gagaatgcag
     1081 gattagcaag tttgcgaatt cgaatatgat cttcaccttc aaggctaagc ataatttcat
     1141 tccaccaagg aactaataag tcgctttcaa ttccattgtg ttctggccaa cgtctcgtgc
     1201 cttgatgaag acgcttgtca ttttgtaatg catttgcatc ttcgtaacga aggatggcta
     1261 cgccataagg cg
//
LOCUS       DBNHVN010000331         2141 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1253348, whole genome shotgun sequence.
ACCESSION   DBNHVN010000331 DBNHVN010000000
VERSION     DBNHVN010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1253348"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="AC16Y1_06400"
     CDS             <1..470
                     /locus_tag="AC16Y1_06400"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR5832125.1"
                     /translation="VALLNMPIEVLEKLVPILLLIASLSVLIPIKPDSGGLTEKQEQA
                     AIFGTGLYCGYFGPGQGVMVAATLARDGRRSSSNLNALKNFITGYTSLVSNLIYLGSG
                     QIQWRLVLLLAVTSGTGGWIGGRAAGRLSLPLYRGLLLLVGVGASAYFFAKFW"
     gene            884..1258
                     /gene="rpsL"
                     /locus_tag="AC16Y1_06405"
     CDS             884..1258
                     /gene="rpsL"
                     /locus_tag="AC16Y1_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007078642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR5832126.1"
                     /translation="MPTIQQLVRKGRQDKVSKNKTPALKGSPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLTSGTEVTAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYKIIRGAL
                     DTQGVKNRKQARSRYGAKKEKS"
     gene            1261..1731
                     /gene="rpsG"
                     /locus_tag="AC16Y1_06410"
     CDS             1261..1731
                     /gene="rpsG"
                     /locus_tag="AC16Y1_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR5832127.1"
                     /translation="MPRKGPAPKRPIVTDPVYASPMVTQLVNKILLHGKKSTAEAIVY
                     GALEGCREKTGTDPLQTLRRALDNVRPTLEVRSRRVGGATYQVPVEVKPARANTLALR
                     WLVGFSRARREKTMTERLMNEILDASNGLGAAVKKREDTHKMAEANKAFAHYRW"
     gene            1810..>2141
                     /locus_tag="AC16Y1_06415"
     CDS             1810..>2141
                     /locus_tag="AC16Y1_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008360969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR5832128.1"
                     /translation="MAHIDAGKTTTTERILFYTGINYKIGEVHEGAATMDWMEQEQER
                     GITITSAATTCSWKDHTINIIDTPGHVDFTVEVERSLRVLDGAVAVFDGVAGVEPQSE
                     TVWRQANK"
BASE COUNT          606 a          488 c          511 g          536 t
ORIGIN      
        1 cagtcgcact tttaaacatg cctattgaag tattagaaaa gttagtccca atacttttac
       61 ttattgcaag cctttcggtg ttaattccaa ttaagccaga ctcaggtgga ctaactgaaa
      121 aacaagagca agctgcaatt tttggaaccg gactttactg tggttacttt ggacctggcc
      181 aaggagttat ggttgctgca acattggcac gcgacggtcg acgctcatcg agcaacctaa
      241 atgcgctaaa gaatttcatc accggttata cctcgttagt atcaaacttg atttacttag
      301 gaagtggcca aattcaatgg cgacttgttt tacttttagc agtgacatcg ggaaccgggg
      361 gatggatagg cggtcgggca gctggccgac tctcgctacc tttatataga ggtctgttgc
      421 ttttggtcgg agtaggagct tcggcctact tttttgctaa attttggtaa ttttttacac
      481 ttcaacgtaa ctaggggaaa gttgaatgcc cacactgaaa aacccctaaa tcattgagga
      541 atttgtccgt ttaggtctga gttcgacagt attggttaag ttggcttaga tcgacacgcc
      601 gaaggtaagc cagcgaacct aactcgtttt gacctagagc acatgttatg ggtaacgtat
      661 tccttcgtgc ccttggaaac ttgggtctcg cacctatgcc tagggcttcc ccgtcggcgc
      721 agattaagcg aaaaaactaa tgttaaaaag ttttttcatg attgcgcagg actgggcgac
      781 actcccgacc gcgtgggtcg aaggtagtga ttggtcacca ccacacaacg aacgaaacga
      841 ttaatgctgc cacgcataaa tcgaatgaca aaaaggagtc agagtgccaa caatccaaca
      901 gttggtccgt aagggtcgac aggataaggt ctccaaaaat aagacacctg ctcttaaggg
      961 cagtccacaa cgtcgtggag tttgcacacg cgtctacaca acaacaccta agaagccaaa
     1021 ctcggcgctt cgtaaagttg ctcgtgtgcg tttaacctct ggcactgaag taaccgcgta
     1081 cattccaggt gaaggtcaca acctgcaaga acattcaatc gttcttgttc gtggcggtcg
     1141 cgtaaaagat ctccctggtg ttcgctacaa aattattcgc ggtgcacttg atactcaagg
     1201 tgttaaaaac cgtaagcaag ctcgcagccg ttatggtgca aagaaggaga agagctagtt
     1261 atgccacgta aaggcccagc tcctaagcga ccaatcgtta ctgatccggt ttacgcatca
     1321 ccgatggtta ctcagttagt taacaagatt ttattgcacg gcaaaaaatc cactgctgaa
     1381 gcaattgttt acggtgcact tgaaggttgc cgtgaaaaaa ctggtactga tccattacaa
     1441 acacttcgtc gcgcattaga taacgtgcgt ccaacacttg aagttcgtag ccgtcgcgtc
     1501 ggtggtgcaa cttaccaagt tccagtagaa gtaaagccag caagagcaaa tactttggcg
     1561 cttcgttggt tagttggatt ctcacgtgcc cgtcgcgaaa aaacaatgac cgaacgttta
     1621 atgaacgaaa ttcttgatgc ttcaaatggc ctcggtgcgg ctgtaaagaa gcgcgaagac
     1681 actcacaaaa tggctgaagc taacaaggct tttgctcact accgctggta aacgaagaag
     1741 agacgagaga acaaaaaagt ggctgcagag acatcacttg acctcgccaa ggtccgcaac
     1801 atcgggatca tggcgcacat tgacgcgggt aaaaccacga ctactgagcg catccttttc
     1861 tacaccggta tcaactacaa aattggtgaa gtgcacgaag gtgccgcaac tatggactgg
     1921 atggagcaag agcaggaacg cggtatcact attacctctg ctgcaactac ttgttcatgg
     1981 aaagaccaca ccatcaacat cattgataca cctggtcacg ttgacttcac tgttgaagta
     2041 gaacgttcac tacgtgtact tgatggtgca gttgcagtat tcgatggcgt agctggagtt
     2101 gaaccacagt ctgaaactgt ttggcgtcag gctaataagt a
//
LOCUS       DBNHVN010000332         1524 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1847578, whole genome shotgun sequence.
ACCESSION   DBNHVN010000332 DBNHVN010000000
VERSION     DBNHVN010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1847578"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..609
                     /gene="rsmD"
                     /locus_tag="AC16Y1_06420"
     CDS             52..609
                     /gene="rsmD"
                     /locus_tag="AC16Y1_06420"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR5832129.1"
                     /translation="MTRIIAGEHRGRALKVPKSVTRPTSSKVREAIFSTVNHALAGLT
                     DLRVLDLYAGSGAFAIEALSRGADEAIAIEIDSRASQTIKDNCETLKLTNLRTLTMDV
                     QAALSGEPQFGKFDLVFIDPPYALADSSITQLLESLVHGWLADGALVVVERAKDSKFV
                     APAELPEFARKVYGDTSVWYGQYED"
     gene            637..1110
                     /gene="coaD"
                     /locus_tag="AC16Y1_06425"
     CDS             637..1110
                     /gene="coaD"
                     /locus_tag="AC16Y1_06425"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007384940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR5832130.1"
                     /translation="MRKAVCPGSFDPVTMGHLDVFARAANQFDEVTVAVLINSTKAGL
                     FSIDERIELIRECVANYKNIKVDSFQGLLVDYCKANDIGAIVKGLRAVSDFDYELQMA
                     QMNSQLGVDTLFVATKPEYSFLSSSIIKEIAKYQGDVAKLVPPAVDRALKAKFNK"
     gene            1224..>1524
                     /locus_tag="AC16Y1_06430"
     CDS             1224..>1524
                     /locus_tag="AC16Y1_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014138240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR5832131.1"
                     /translation="MSSVDPRSPLVLDTRELGRGAGSMKSYKFVAPAPTELGIAMIAV
                     PTGSEIEFEIRLESVVDGVLVSGTALVSVEGECSRCLEPIAFDMEVDLQELFEYPP"
BASE COUNT          437 a          310 c          355 g          422 t
ORIGIN      
        1 cgcagtgaca aacttaattg aaacccaaca agcacagttt gtgaaaaaat catgacccga
       61 attattgctg gtgagcatag aggacgggca ctaaaagttc ccaagtcagt gactcggcca
      121 acttcaagca aagtacgcga agcaattttt tcaactgtta atcatgcgtt agctggctta
      181 actgatctgc gcgtacttga tctttatgct ggatccggag cctttgcaat tgaggcgctt
      241 tctcgcggtg ctgatgaagc cattgcaatc gaaatagatt ccagagcttc gcaaactata
      301 aaagacaatt gcgaaacttt gaaattaaca aatcttcgaa ctttgacgat ggatgtgcaa
      361 gcggcacttt caggcgaacc acaatttggc aaatttgatc tagtttttat tgatccacct
      421 tacgcacttg cagatagctc aataacgcag ctacttgaat ctcttgttca tggttggttg
      481 gcagatggtg cattagtggt agttgaacgc gcaaaggatt ctaagtttgt agcgcctgca
      541 gaattacctg aatttgcgcg caaggtttac ggcgacacaa gtgtttggta cggtcagtat
      601 gaagattaag ccaattagct caagtaaggc ctgaagatgc gcaaagcagt atgtcctgga
      661 tcctttgatc cagtcaccat gggacacctt gatgtttttg cacgagcagc gaatcaattt
      721 gacgaagtta ccgtagccgt tttaattaac agtacaaagg ctggactttt ctcaattgac
      781 gaacgcattg aactgatccg agaatgtgtg gcgaactaca aaaacattaa agttgattct
      841 tttcaaggcc ttctagtcga ttactgcaaa gcaaatgaca tcggtgcaat tgtaaaaggt
      901 ttacgtgccg tttctgattt tgattatgaa ttacaaatgg cacagatgaa ttctcaactg
      961 ggtgttgaca cactttttgt ggcaacaaag cccgagtaca gctttctgtc ttcgagcatc
     1021 attaaagaaa ttgctaagta ccaaggtgac gttgctaagt tggtgccgcc agcagttgac
     1081 cgagcgctga aggctaaatt taataaatag tctttatttc aaaggtaatt gtcccggccg
     1141 gatttggggc gaacctaaaa attaaggtaa cctagccatt ggtcaggcta gaccaaacag
     1201 tagcgaattc cagcgaggat gccatgtctt ctgttgatcc acgctcgccg ttagtactcg
     1261 atactcgaga actcggacga ggtgccggct caatgaaatc atacaaattc gttgcaccag
     1321 cgccaactga acttgggata gccatgattg cagttccaac cggatctgaa attgagtttg
     1381 aaatcagact cgagtcagtg gtggatggag ttctcgttag cggtactgca ttggtatctg
     1441 ttgaaggaga atgttcaaga tgtcttgagc cgattgcttt cgatatggaa gtagatctac
     1501 aagaactttt tgaatatccg ccaa
//
LOCUS       DBNHVN010000333         2306 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_288631, whole genome shotgun sequence.
ACCESSION   DBNHVN010000333 DBNHVN010000000
VERSION     DBNHVN010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_288631"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="AC16Y1_06435"
     CDS             <1..288
                     /locus_tag="AC16Y1_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5832132.1"
                     /translation="SPVSWIHHIAWLVAVPGAILKTEQLKTDLGKVSRTGMLWFFTLF
                     VMAIPPVRYGWSPIRWIGLEEHYSFICVLIILLLWWRSKHNSQQTQNYVKS"
     gene            301..819
                     /locus_tag="AC16Y1_06440"
     CDS             301..819
                     /locus_tag="AC16Y1_06440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR5832133.1"
                     /translation="MTTLFTLHTNHGDIRINIFDNHAPKTCKTILGLADGTHEWTDPR
                     TNEKTTKPLYDGTIFHRIIPGFMIQGGDPLGQGIGGPGFKFADEPHPELVFDRPYLLA
                     MANAGPNTNGSQFFITVAPTTWLNFKHTIFGEVADQESRDVVDAIANVATGAMDRPVE
                     DVVIEKTTVTKS"
     gene            900..1622
                     /locus_tag="AC16Y1_06445"
     CDS             900..1622
                     /locus_tag="AC16Y1_06445"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR5832134.1"
                     /translation="MVSAPVGFQCVECVNQAPKPKVRSSFGAPTSQIPIVTKYLIGIC
                     VGAFIYMMLNGGVYYLANRFGMIPLAIASGEWWRLLTAAFLHGSFLHIAFNMYALYWL
                     GPQLENFLGRARFLGLYMLSALGGSVASYYFSDIQTVSVGASGAIFGLLTATIVIGRE
                     LRHDVSQLTVLLAINVGLGFFSSAIDWRAHLGGAAVGAAIGYLQTRGNKEFRLVGMIG
                     ITVALLGIALLRGQQITSLFTG"
     gene            1670..1936
                     /locus_tag="AC16Y1_06450"
     CDS             1670..1936
                     /locus_tag="AC16Y1_06450"
                     /inference="COORDINATES: protein motif:HMM:NF018487.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HIR5832135.1"
                     /translation="MPESKRRKSLKPAYVPPTSTNQTPVKIGSPVWLVPTMVGMFIVG
                     LLWIVVFYLAGNQVPVMRDLNNLLNVGIGFGFIGIGFALATKWR"
     gene            1999..>2306
                     /locus_tag="AC16Y1_06455"
     CDS             1999..>2306
                     /locus_tag="AC16Y1_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HIR5832136.1"
                     /translation="MNRSNSRRARRAITALIALSAGLLFVSSGNTAAGTNIRTSGLED
                     LRSLIINRAKGMVGLEQDVNALQTEVGQLTEGKIDPELSAQLAALENQAGLSPVSGAA
                     L"
BASE COUNT          640 a          473 c          520 g          673 t
ORIGIN      
        1 tcccctgtct catggatcca ccacatagcg tggttagttg ctgttccagg tgcaatttta
       61 aaaactgaac aactcaaaac agatctaggt aaagtttcaa gaacaggcat gctttggttt
      121 tttaccttat ttgtgatggc aataccacct gttcgatatg gttggtcacc cattcgatgg
      181 attggacttg aggagcatta cagttttatt tgtgttttaa ttattttgtt gttgtggtgg
      241 cgtagtaaac ataatagtca gcaaacacaa aattatgtta agtcttaaaa aaaggaaaaa
      301 atgacaactt tatttacact ccacacgaat catggtgaca tccgaatcaa catttttgat
      361 aatcatgcgc caaaaacttg caaaactatc ttgggtttag ctgatggcac gcatgagtgg
      421 actgacccac gcacgaatga aaaaacaact aagccacttt acgatggcac aatttttcat
      481 cgaatcattc caggtttcat gattcaaggt ggagatccat taggccaggg gatcggcggc
      541 ccgggcttta aatttgcaga tgagccacat ccagaattag tttttgaccg tccttattta
      601 cttgcaatgg caaatgctgg acctaatacc aacggttcac agttcttcat caccgttgca
      661 ccaactactt ggttgaattt caaacacact atttttggcg aagttgctga tcaagaaagc
      721 cgcgatgttg ttgacgcaat agcaaacgta gctactggtg caatggatcg accagttgaa
      781 gatgtggtaa ttgaaaagac aactgttacc aaaagctaat gacgacctgc taccggcact
      841 ctgatcgaga aacatatatt gaatgttcta ggtgtgggca atttatttgc ccagagtgca
      901 tggttagcgc acccgttggt tttcaatgtg tggaatgcgt aaatcaagcg ccaaaaccta
      961 aagttcgttc aagttttggt gcaccaacaa gtcaaatacc aatcgtcacc aaatatttga
     1021 ttggtatttg cgttggtgcg tttatctaca tgatgttaaa cggtggcgtt tactacttag
     1081 caaatcgttt tggcatgatc ccgctggcga ttgctagcgg tgaatggtgg cgtttactta
     1141 ccgctgcatt tttacatggc agctttttac acatagcttt taacatgtat gcgctctatt
     1201 ggcttggacc acagctagaa aattttcttg gtcgagcaag atttctaggg ttgtatatgc
     1261 tctcagcact tggcggcagc gttgcgtcct actatttttc agatattcaa actgtttcag
     1321 ttggggcctc gggcgcaatt tttggtttgt taaccgcaac aattgtgatc ggccgagaac
     1381 tacgacatga cgtctcgcaa ctcaccgtac ttcttgcgat aaacgttggt ctaggctttt
     1441 tcagttccgc aattgattgg cgagcccacc taggtggcgc agccgtaggt gcagcgattg
     1501 gctatttaca aactcggggt aataaggaat ttcgattagt gggaatgatt ggaattactg
     1561 tggcactact cggcattgct ttactaaggg gccaacaaat aactagcctt tttacaggtt
     1621 aagtaaattc ccgattacca tcagggcaaa caataagtaa gattgagtta tgccagaatc
     1681 aaaacgtcgg aaatcgctga aaccggccta cgtgccacca acttcgacaa atcaaactcc
     1741 agtaaaaatt ggaagtccag tttggttagt tccaactatg gttggcatgt ttattgtcgg
     1801 attactttgg atcgtagttt tttatcttgc cggcaatcaa gttccagtca tgcgggattt
     1861 aaataatttg ttaaatgttg gaattggctt tggatttatt ggaatcggct tcgcgcttgc
     1921 gacgaagtgg cgctgaaact cctttaatta caccggtgta atttctcccc aacggtggat
     1981 aaggttgtgg ataagtccat gaatagatca aactccagac gagcccgccg agcaatcact
     2041 gcgctgattg ccttatcggc cggattactt tttgtttcga gcggtaacac tgcagctggc
     2101 acaaacatcc gtacaagtgg cctggaagat ttacgaagtc taataattaa tcgagccaaa
     2161 ggcatggttg gtttagagca agatgtaaac gcgctccaaa ctgaagttgg tcaattaacc
     2221 gaaggaaaaa ttgatccaga gttaagtgct caacttgctg ccctggaaaa ccaagcaggt
     2281 ctatcgccag taagtggagc agcact
//
LOCUS       DBNHVN010000334         1302 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1699840, whole genome shotgun sequence.
ACCESSION   DBNHVN010000334 DBNHVN010000000
VERSION     DBNHVN010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1699840"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..271
                     /locus_tag="AC16Y1_06460"
     CDS             134..271
                     /locus_tag="AC16Y1_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019852316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2256 domain-containing protein"
                     /protein_id="HIR5832137.1"
                     /translation="MRVAKAKNGFAPKVCQTCGLPFEWRKKWAKNWDSVKYCSEKCRK
                     S"
     gene            271..>1302
                     /locus_tag="AC16Y1_06465"
     CDS             271..>1302
                     /locus_tag="AC16Y1_06465"
                     /inference="COORDINATES: protein motif:HMM:NF016158.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptochrome/photolyase family protein"
                     /protein_id="HIR5832138.1"
                     /translation="MSKLIYVAFDQLNKNYGALKSAKPKEDIVVMVESQRMVVGRKWH
                     KQRLHFLISSARHFAHELEQAGFKVLYLKSSTTKEGLTELKKKYPKHELVCAAPNSFK
                     LRHDLIALGATVLDSDFFLTSEHLFKLWADAQKTYTMENFYRAQRRRLNILMVGDKPE
                     GGTWNYDAENRLPPPKVHDWGKTLTFKSDEIDAQVKAELPDTAWGELEQKIWGTSRAE
                     ALKQMKHFFDNHFKDFGPYEDAMPNSSWSAHHSLLSPYLNNGLLHPSEVVKAALKKYE
                     SGEVSIESCEGFVRQIIGWREYVNGMYWYLGEDYRDNNGLGADRKLLPLFHDPNKTQM
                     NCVKSVVTDI"
BASE COUNT          394 a          259 c          294 g          355 t
ORIGIN      
        1 gttgatcagc gctttttcac ttggtgtatt gaccctactg attccagttg cacttagtcg
       61 gttcacggtt ttgtatgcct taattggatt tggcttttct gcggcttatg cgttcgcgat
      121 gtggaaaaaa taaatgcggg tagctaaagc taagaatgga tttgcaccta aagtatgcca
      181 gacctgcgga ttaccatttg agtggcgaaa aaaatgggca aagaattggg attcagttaa
      241 atactgctca gaaaaatgtc ggaaaagttg atgagcaaat taatttatgt tgcctttgat
      301 cagctaaata aaaactacgg tgccttaaaa agtgccaaac ctaaagaaga tatcgtagtt
      361 atggtcgaaa gccaacgaat ggttgtcggc cgaaagtggc acaagcagcg tttacatttt
      421 ttaatctcta gtgcccgcca tttcgctcac gagcttgaac aggctggatt caaagtttta
      481 taccttaaaa gctcaaccac caaggaaggg ctgactgagc tgaagaagaa atacccgaaa
      541 cacgaactag tttgtgctgc gcctaactca tttaagttac gccatgactt gatcgcactt
      601 ggggcaactg ttctagacag tgattttttt cttaccagcg aacatttatt caagctctgg
      661 gctgacgcgc aaaaaactta cacgatggaa aatttttatc gtgcccaacg tcgccgctta
      721 aacatcctga tggttggtga caaaccagaa ggtggcactt ggaattacga tgccgaaaac
      781 cgactaccac caccaaaagt tcatgactgg ggcaaaacgc taacttttaa atccgatgag
      841 attgatgcgc aagtaaaagc tgaactgccc gataccgcgt ggggcgaact tgagcaaaaa
      901 atctggggca catcgcgtgc cgaagcacta aagcaaatga aacatttttt tgacaatcac
      961 tttaaggact ttggccctta tgaagatgcg atgccaaatt cgagttggag tgcacaccac
     1021 tcacttttaa gtccttattt aaataatgga ctacttcatc ccagtgaagt tgttaaagct
     1081 gctctcaaaa aatacgagtc aggtgaagtc tcaattgaat cttgtgaagg atttgttaga
     1141 cagattattg gttggcgcga atatgtaaac ggaatgtatt ggtacttggg cgaagattat
     1201 cgcgataaca atggcttagg tgcggataga aagttacttc cactttttca cgaccccaac
     1261 aaaacgcaaa tgaactgtgt gaagtccgtg gttactgata tt
//
LOCUS       DBNHVN010000335         4227 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_67185, whole genome shotgun sequence.
ACCESSION   DBNHVN010000335 DBNHVN010000000
VERSION     DBNHVN010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_67185"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2348)
                     /locus_tag="AC16Y1_06470"
     CDS             complement(<1..2348)
                     /locus_tag="AC16Y1_06470"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013863085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HIR5832139.1"
                     /translation="MAKSTTSSKNQEHGPGGRIVDIDVASEMQGSFLEYAYSVIYSRA
                     LPDARDGLKPVQRRILHTMAQMNLKSDRGHVKSARVVGEVMGKLHPHGDSAIYDALVR
                     MAQSWSLRLPFVDGHGNFGSLDEGPAAYRYTEARLAAAANAMVDSLDEDTVDFNPNYD
                     GRELEPAVLPAAIPSLLVNGASGIAVGMATNMPPHNLREVTAAAVHLLNNPKATLDDL
                     MEFVPGPDLPTGGLIVGLEGIREAYESGKGSFRVRARVDIERVSAKRTGLVVRELPYQ
                     VGPERIIERIKDLVTSKKLQGISDVIDLTDYESGLELVIELKNGFNPQAVLDQLYKQT
                     PLEDSFHINNVTLVEGQPVTLGLKALLDVFVKHRLEVVKRRSEFRRKKAQDRLHLVDG
                     LLIAILDIDKIIKLIRASEDTGTARTGLMKTFKLSELQANHILEMPLRRLTKFSKLEL
                     QKEQKELQAIIADLTEILTSSKRLKKVVAQELNDMADLYGDDRRSDLISDDEIISAAS
                     SAPMEVEDDPCYVIMSTSGLLARTSSLAPLEIGKKRVNHDAIRSVVKSSARGEIGLVT
                     SAGRVLRLSVIGLPALPENANLTAAGGGAIKEFLTLPKDEKPLALMNLADSNSTLVVF
                     TASGQIKRVNHDVPASAKDWNILKLDNQDSLVGAIQSTDDSGNAILITSDAQLLHFPA
                     NTVRGTGRQAGCVAGIKLAKGAKVIYGSVINPDLDPVVATVAGKDEANATSIKVSQLS
                     EFPAKGRATGGVRCHKFLAGENVLVTAAAGSAPIHAATGTGG"
     gene            complement(2399..3193)
                     /gene="sepH"
                     /locus_tag="AC16Y1_06475"
     CDS             complement(2399..3193)
                     /gene="sepH"
                     /locus_tag="AC16Y1_06475"
                     /inference="COORDINATES: protein motif:HMM:NF040712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein SepH"
                     /protein_id="HIR5832140.1"
                     /translation="MKDLIFVGRSDDNSALVFMDDNGEEFRVAVDDHIVRNVNSPKTA
                     SAGETSLGISPRDIQTRIRRGESPKEIAIDAGVSVERIERYAGPVYAERLYISTKARE
                     TFVRRPHGEMRLEDIVVSQLISRNVDILTLEWDSYRRDDNRWTITVQWASGTGTGGAS
                     WIYDPLGHSIVALDDEARWLFDEATSAAKEVNRKIAAAEPKPRLVGLPTVAQDVEPEA
                     ELDSPGWAGPGHPTIPVPMPVSNPAPIVDDSPSWDDILFGHRPSDQ"
     gene            3270..4139
                     /locus_tag="AC16Y1_06480"
     CDS             3270..4139
                     /locus_tag="AC16Y1_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015036716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR5832141.1"
                     /translation="MVNPKTRNDGTMTKATQDYEKLLTDPDVAFAVDLARRAGDLLAD
                     RPIALTVETKTTDTDVVTHMDQMAEELIVGAIEIGRPQDGLLGEEGADKKSSSGRQWV
                     IDPIDGTVNYLYQLPHWCVSIALVEEETQRGIVGVVSAPSLNSLFVSRAGQGAFRISN
                     NQVSRLQVSTEDRLSHSLVGTGFGYSSIRRAHQAKVLTQILPNVRDIRRLGSCALDLC
                     LVASGELDAYYERGVKPWDHAAGSLMVVEAGGKVSDLDDQRPSEKMIVAGNSETQPAL
                     VKILKTQNADQGD"
     gene            4142..>4227
                     /locus_tag="AC16Y1_06485"
     CDS             4142..>4227
                     /locus_tag="AC16Y1_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011598384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIR5832142.1"
                     /translation="MPIYALGDKVPNIHADAFVHPDAVIIGDV"
BASE COUNT         1141 a          997 c          961 g         1128 t
ORIGIN      
        1 aatccaccag ttccagtggc tgcatgtatt ggtgcactac cggcagcggc tgtgacaaga
       61 acattttcgc cagctaggaa cttatgacaa cggacaccac ctgtagctcg gcctttagcg
      121 ggaaattcgc taagttgcga aactttaata ctggttgcat tagcttcatc tttacctgca
      181 acagttgcga ccaccggatc gagatccggg ttaataactg agccatagat tactttcgca
      241 cctttagcca acttgatacc tgccacacat cctgcttggc gtcccgtacc tctaactgtg
      301 ttggcgggga agtggagaag ttgcgcatca ctcgtaatca aaattgcatt tcctgagtca
      361 tctgttgatt gaatagcccc aaccaaagaa tcttgattat ccagtttgag aatattccag
      421 tctttagcac tagctggcac atcatgattc actcgtttga tctgaccact ggcggtaaaa
      481 acaactaaag tcgaattact gtcagccaaa ttcatcaagg caagtggctt ttcatcctta
      541 ggaagagtta ggaattcttt tattgcgcca ccaccggcgg cggttaagtt cgcattttcc
      601 ggtaacgcag gtaaaccaat aaccgaaagt cgcagcactc gaccagcact ggtcaccaag
      661 ccaatctcac ccctggctga tgatttaacc actgagcgga ttgcgtcatg attaacccgt
      721 tttttaccga tttccaaagg cgcaagagaa gaagtccgag caagtaatcc gctcgtagac
      781 atgatcacat aacaaggatc atcttcgact tccatcggcg ctgacgaggc agcagaaatt
      841 atttcatcat cagaaattaa atcgctgcgg cggtcatcac cgtataagtc cgccatgtca
      901 ttaagttctt gcgcaacaac ttttttaagt cgcttactgc tagttagaat ttcagttaaa
      961 tcggcaatga ttgcctgcaa ttctttttgt tccttttgaa gttctaattt tgaaaactta
     1021 gttaatctgc gcaaaggcat ttccagaatg tgattggcct gtaactcgct taacttaaag
     1081 gttttcataa ggccagttcg tgccgtacca gtatcttcac ttgcgcgaat taacttaata
     1141 attttgtcta tatctaaaat tgcaattaat agaccgtcaa ctaagtgcag tctgtcttgc
     1201 gcttttttgc gacggaattc tgagcggcgc tttactactt ctaaacgatg tttcacgaaa
     1261 acatcaagga gtgccttcaa cccgagagtg actggctgac cttctactag agtcacgtta
     1321 ttgatatgaa atgaatcttc tagcggagtt tgcttatata gctgatcaag cactgcttgt
     1381 gggttaaatc catttttcag ctcaatgaca agctctaaac cggattcata gtccgttaaa
     1441 tcaatcacat cagaaatgcc ttgcagtttc ttactggtaa ctaaatcttt aatacgttca
     1501 ataatgcgct ctggtccaac ttgataaggc aattcacgaa ccactagacc agtgcgtttt
     1561 gcggaaactc tttcgatatc tactcgggca cgaacacgga agctgccttt acctgattca
     1621 taagcttctc ggataccttc aagaccaaca atcaacccgc cggtcggtaa atctggtcca
     1681 gggacaaact ccattaagtc gtccaaagtt gcttttggat tattaagtag atgcactgcg
     1741 gctgcagtaa cttcacgcaa attatgtggt ggcatgtttg tggccatacc aacggcaata
     1801 cctgaagcac cgttaaccaa tagtgagggg atagcagcag gcaaaactgc tggttctaat
     1861 tcgcggccgt catagtttgg gttgaaatca actgtgtctt catccagcga atccaccatt
     1921 gcattcgccg cagcagctaa ccgtgcttcg gtatatcgat aagcagcggg accttcgtct
     1981 aaggaaccaa agttgccgtg gccatcaaca aaaggaagac gcaatgacca agattgcgcc
     2041 attcgaacca acgcatcgta aattgcactg tcgccgtgcg gatgcaattt acccatgact
     2101 tcgcccacaa cacgggcaga tttaacatgg cctcggtcgg attttaaatt catttgcgcc
     2161 atagtgtgca agatgcgccg ctgtacgggc tttaatccat cacgtgcatc aggcagtgca
     2221 cgtgaataga ttacggagta ggcgtactca aggaatgaac cctgcatctc gcttgcgaca
     2281 tcaatgtcga caattcgtcc gcctggaccg tgttcttgat tcttacttga cgtggttgac
     2341 ttagccattt tctcgcttat ttttagtttc taagattgct tacttgaatt cgagcacttt
     2401 attgatctga aggacgatga ccaaacaata tgtcatccca ggaaggtgag tcatcaacaa
     2461 taggtgccgg atttgaaact ggcatcggaa ccggaattgt tggatgtcca ggaccagccc
     2521 aaccaggact gtctagttcc gcttctggtt caacgtcttg ggcaactgtg ggcaaaccaa
     2581 caagtcgggg tttaggttca gctgctgcaa tttttcgatt aacttcttta gctgcacttg
     2641 ttgcttcatc aaatagccaa cgagcttcat catctaaagc aactatcgaa tgaccaagcg
     2701 gatcataaat ccatgaggct ccgccggtcc cagttcctga tgcccactga actgttattg
     2761 tccaacggtt gtcatcgcgg cgataggaat cccattcaag ggtaagaatg tctacattgc
     2821 gcgaaataag ttggctgacc actatgtctt ctaggcgcat ttcgccatga ggccggcgca
     2881 caaaagtttc tctagctttt gttgaaatgt ataaccgttc agcataaaca ggcccggcat
     2941 aacgttctat gcgctcaaca gaaaccccag cgtcaattgc aatttctttt ggagattcgc
     3001 cccgacggat gcgagtttga atatcgcgtg gcgagattcc tagtgaagtt tctccggcac
     3061 ttgcggtctt aggagaatta acattgcgaa ctatgtgatc atcaacggcg acgcgaaatt
     3121 cttcgccatt gtcatccata aagaccaggg cggagttgtc atcacttcga ccaacaaaga
     3181 taaggtcttt caagtttctt cctccaaagc tctacgaata gtttcgcgtg aaaagccctg
     3241 aaaatcgggg tttttggggg gtgtgtcgca tggttaatcc caagacgcgc aatgatggaa
     3301 ccatgacaaa agccacccag gattatgaaa agttactcac cgatccggat gtggcattcg
     3361 ccgttgactt ggctcgccga gccggcgatt tacttgccga tcggccgatt gcactgacag
     3421 tagaaaccaa aactactgat accgacgtgg ttactcacat ggaccaaatg gctgaagaat
     3481 taattgttgg tgcgattgaa attggtagac cacaagatgg gttacttggc gaagaaggtg
     3541 cagacaaaaa gagttcatca ggtagacagt gggtgataga tccaattgac ggaaccgtta
     3601 attatctcta tcaattgcca cattggtgtg tatctatagc tctagttgag gaagagaccc
     3661 agcgtggaat agtgggcgtt gttagtgcac caagtcttaa ctcacttttc gttagccgag
     3721 cgggtcaagg cgcatttcgg atttccaaca accaggtttc taggctgcaa gtttcaaccg
     3781 aagatcgact gagccacagt ctggttggca ctggatttgg ctattcctcg attcgacgag
     3841 cgcatcaagc caaagtgtta acgcaaatat tgcccaatgt acgagacatt cgacggctag
     3901 gctcatgcgc actcgatcta tgcctggtag caagtggaga actcgatgca tattatgagc
     3961 ggggagttaa gccttgggat catgccgcgg gctcgctcat ggtggtcgaa gctggcggga
     4021 aagtaagtga cctagacgat caaaggccaa gtgaaaagat gatcgttgcg ggtaatagtg
     4081 aaacacaacc tgccttagtc aaaatcttaa aaacccaaaa tgcagatcaa ggagactagc
     4141 aatgcccatt tatgcactcg gcgataaagt cccgaacatc cacgcagacg cattcgtgca
     4201 tccagatgcg gtaattattg gcgatgt
//
LOCUS       DBNHVN010000336         1065 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1477428, whole genome shotgun sequence.
ACCESSION   DBNHVN010000336 DBNHVN010000000
VERSION     DBNHVN010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1065)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1065
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1477428"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="AC16Y1_06490"
     CDS             <1..1007
                     /locus_tag="AC16Y1_06490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR5832143.1"
                     /translation="LLVGTGRGAALGLLIRGPRAIESSSRIDQMFIDKTGTLTDGQMS
                     VSDVVSTISDQELWQIVDALEATSMHPVATSLRKHAHTLGFTSNPATSVQSVSGSGVQ
                     GLVNGVPCALGSVSWLGVPAGELKTAADKFAAQGDGVVVVYRNAFAVAVIALSDQIAA
                     NTKTAIENLAKMNIEPIVISGDHEAAVKKVCDQLGIASYHANSSPEYKLEQVTLHQSQ
                     GHHVAMVGDGVNDAAALAKAHLSLAMGAGTDVAASSADIVLMRSSMAAAVDAIALSRA
                     TMKTIRSNLFWAFAYNVAAIPLAMAGLLGPIIASAAMAFSSVFVVTNSLRLRKFQPIK
                     "
BASE COUNT          284 a          227 c          260 g          294 t
ORIGIN      
        1 cgttacttgt tggcactggt cgaggcgcag cacttggttt actgattcgc ggacctcggg
       61 caattgaatc aagtagccga atagatcaaa tgtttattga taaaactggc acattaactg
      121 acgggcaaat gagtgtttca gatgtagtta gcaccatttc tgatcaagag ctttggcaga
      181 tagtggatgc gctagaggca acttcaatgc atccagtagc cactagtttg cgaaagcatg
      241 cacacactct tggattcacc agtaatccag ccacgagtgt gcaaagtgtc agcggttcag
      301 gtgttcaagg tttagtcaat ggagttccat gcgcactagg ttcagtttct tggcttggcg
      361 tgccagcagg cgaactcaaa actgctgcag ataaatttgc cgcccagggt gacggcgtag
      421 ttgtggttta ccgaaatgca tttgccgttg cagtaattgc tctttcagat caaatagcgg
      481 ctaacactaa aaccgccatt gaaaacttag ccaaaatgaa cattgaacca attgtgattt
      541 ctggtgacca cgaagcagca gttaaaaaag tttgtgatca attaggtatc gcaagttacc
      601 atgcgaatag ctcaccggaa tacaaattag aacaagttac tcttcatcaa agtcaaggtc
      661 atcacgttgc catggttggc gatggagtca atgatgcggc ggcgttggct aaggcgcatt
      721 taagtttggc catgggtgct ggcacagatg tggctgcaag tagtgcggat attgtcctca
      781 tgcgctcatc gatggccgca gcagttgatg caattgctct ttcgcgggcg acgatgaaaa
      841 caatccgctc aaaccttttc tgggcttttg cttataacgt ggcagcaatt ccacttgcaa
      901 tggctggttt gctcggccca attattgctt cggcagcaat ggcattttcc agtgtctttg
      961 tagttactaa tagtttacgg ctgcgaaaat ttcagccaat caagtaagga ttaattaaat
     1021 ccagtttgga aaccacatcc gtagtttcca catttcgtaa gttaa
//
LOCUS       DBNHVN010000337         2952 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_2071614, whole genome shotgun sequence.
ACCESSION   DBNHVN010000337 DBNHVN010000000
VERSION     DBNHVN010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_2071614"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="AC16Y1_06495"
     CDS             complement(<1..880)
                     /locus_tag="AC16Y1_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR5832144.1"
                     /translation="MASPKKSTSKKSTAKKSSAVKKTVGKKAVARKKSANKSGVKKTT
                     AKSPVKKKSSAKKSAPIKAGKKSKSKSTAKAPAKLTPKQREAARKALAREKEAARKAA
                     AKAKEAARKEAAKQKELARKEAIKKKEAAKKEAALIAAAKKAEKEAARAAKEAAKQAA
                     KIVIPPPPAVQVINPIGGDTIPQVRKAVPGKKAAPKRPVVEEDESKWTKTELNQVRKI
                     LLKEVAELDAEIAAEEARFHALIVESGEGAGDDQADAGAKTFEREHEISILSNKKDLL
                     DQSHHALERIEAGTYGF"
     gene            1310..>2952
                     /locus_tag="AC16Y1_06500"
     CDS             1310..>2952
                     /locus_tag="AC16Y1_06500"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020699972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR5832145.1"
                     /translation="MSYRDVPAKIDLPKMEQDVLAFWASNDIFHKSLKRTQDGPLWVF
                     FEGPPTANGMPGTHHVEARVFKDIFPRYRTMKGFNVPRKAGWDCHGLPVELAVEKELG
                     FNGKQDIEKYGIAQFNEKCRESVLKYVGAFGDMTTRMGYWVDMDQAYWTMDENYIDSV
                     WWSLKRIFDKGLLVQDYRVSPYCPRCGTGLSDHELAQGYEDVVDASVYVRFPVTSGKL
                     LDKYPGLNFLVWTTTPWTLISNTAAAVNPEVDYVVALDSNNEHFVLASALVEKVLGED
                     AKVLETFSGKSLEGTSYNRPFDWVSFPENPGPVNTIVLASCVTTEDGTGIVHQSPAFG
                     AEDLAVCKSYGLPMINPVAPDGTFEADIPEVGGVFFKAADPRLVEILKERGLLFRHLP
                     YEHAYPHCWRCHTALIYYAQPSWYIRTTEIKDQLVAQNEKTNWFPETIKWGRYGDWLN
                     NNVDWALSRNRYWGTPLPIWRCTEGHLTCVSGRRELAQLAGDAALTVDPHRPYVDDVT
                     FACPECSTEATRVPEVIDCWFDSGAMPFAQFGYPELNKDA"
BASE COUNT          719 a          650 c          692 g          891 t
ORIGIN      
        1 aaaaaccgta agttccagct tcgatccgct ctagtgcatg gtgcgactga tcgagtaaat
       61 ctttcttgtt ggaaagaatg gaaatttcat gttcgcgttc aaatgtcttt gcgccagcat
      121 ctgcttggtc gtcgcctgca ccttcgccac tttcaacaat taatgcatgg aagcgtgctt
      181 cttcggcagc gatttcagca tcaagttcgg caacttcttt aagtaaaatt tttcgaactt
      241 ggtttaattc cgttttagtc cactttgatt cgtcttcttc gacaacaggt cgttttggcg
      301 cggctttctt tccgggaact gcttttcgaa cttgtggaat tgtgtcgcca ccaattgggt
      361 taatcacttg aacggctggc gggggtggaa taacaatttt tgcggcttgc ttggccgctt
      421 cctttgctgc tcgggcagct tctttttcgg ctttctttgc cgctgcaatt aacgcagctt
      481 cttttttcgc agcttctttt ttcttaattg cctctttgcg ggctaactct ttttgcttcg
      541 ctgcttcttt acgggcagct tcttttgcct tcgcagccgc tttgcgagca gcttcttttt
      601 cgcgggctaa tgctttgcgg gcggcttctc tttgttttgg cgttaatttc gctggcgcct
      661 ttgcagttga cttggattta gatttttttc cagctttaat cggagcagat tttttggccg
      721 aactcttctt ctttacaggt gacttagcgg tggtcttttt tacgccagac ttgttggcac
      781 ttttcttcct tgccacagct tttttgccca cagtcttttt aactgccgaa ctcttctttg
      841 cagtgctttt ctttgaagtg cttttctttg ggctagccat tattacttac tcctcccgag
      901 acataagttt taacaattct cgttactcta cctaaaacta atgacttgga ggcaaagagc
      961 caccccagga atggtttatt ttatgggctt gccaaccatt ctggttttta aaccccatta
     1021 tccacagggt aaactcgtgc ccagttcgaa actgacgatg accgaggaca agtcaagcag
     1081 ttcagctgat taaagcgagc cagggttggt gtgagcctgg tagtcagccc tgattgatat
     1141 cacctggcga gttagccgaa ctaaaaacta agtgggttaa taaatattgt tagtaaatat
     1201 tgttagccaa ccagggtggt accgcgggct tgctcgtccc taactcaact gcacgaaact
     1261 gatccgaacg aacgcataaa actaacacat aaaaattgag taggaataaa tgagctaccg
     1321 ggacgtacct gcgaaaattg atctgccaaa gatggaacaa gatgttctag ctttttgggc
     1381 aagtaacgat atttttcaca agtcactcaa gcgcacccaa gatggtccac tgtgggtttt
     1441 cttcgaaggt ccgcctaccg caaacggtat gcctggtaca catcacgtcg aagcacgcgt
     1501 atttaaagat atttttcctc gctaccgcac aatgaagggg ttcaacgttc cgcgtaaagc
     1561 ggggtgggac tgtcacggtt taccagttga gttagccgtc gaaaaagaac ttggtttcaa
     1621 tggcaagcaa gacattgaaa aatacgggat tgcacaattc aacgaaaaat gtcgcgaatc
     1681 agttttgaag tatgtaggcg catttggtga catgacaact cgaatgggtt actgggttga
     1741 catggatcag gcgtattgga ctatggatga aaattacatc gacagtgttt ggtggagtct
     1801 aaaacgaatt ttcgataaag gtttgttagt tcaggactat cgggtttccc catattgccc
     1861 acgttgtggc actggcttat ctgatcacga gttggcgcaa ggttatgaag atgttgttga
     1921 tgcatcggtc tacgtgcgct ttccggttac tagtggcaag ttactggata agtacccagg
     1981 gttgaatttc ctagtttgga ctacaactcc gtggacccta atttcaaaca ctgccgcagc
     2041 agtaaacccc gaagtagatt atgtagtcgc gctagacagc aataatgaac actttgtttt
     2101 agcaagtgca ttagttgaaa aagttcttgg cgaagatgcg aaagtattag aaaccttcag
     2161 tggaaaatct cttgagggta cgagttacaa ccgaccattt gattgggtta gttttcccga
     2221 aaatccagga ccagttaaca caattgtgtt agcaagttgc gtcacaactg aagacggcac
     2281 aggcattgtg caccaatcac cggcgttcgg tgccgaggac ttagcggtat gtaagtcata
     2341 cggtctgcca atgattaacc cggttgcccc tgatggaaca ttcgaagctg acattcctga
     2401 agttggtggc gtatttttca aagctgctga tccgcgatta gtcgaaatac tcaaagaacg
     2461 tggtttactt tttcggcact tgccatatga acacgcttac ccacattgct ggcgttgtca
     2521 cactgcactt atctattacg cccaaccttc gtggtacatc cgcacaactg aaattaaaga
     2581 tcaactagtt gcgcaaaatg aaaaaacaaa ttggtttcca gaaacaatta aatggggtcg
     2641 ttatggtgac tggctcaaca acaatgttga ctgggcactt tctagaaatc gttactgggg
     2701 tacgccgcta ccaatttggc gttgcaccga aggtcactta acgtgcgtaa gtggtcgacg
     2761 tgaactggcg cagttggctg gtgatgcggc actgacagtt gatccacatc gcccgtacgt
     2821 ggatgatgta acttttgctt gtcctgaatg tagtactgag gcaacacgtg ttcctgaagt
     2881 aattgactgc tggtttgatt caggtgcgat gccatttgcg caatttggat atccggaact
     2941 taataaagat gc
//
LOCUS       DBNHVN010000338         1233 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1106418, whole genome shotgun sequence.
ACCESSION   DBNHVN010000338 DBNHVN010000000
VERSION     DBNHVN010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1106418"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="AC16Y1_06505"
     CDS             <1..1108
                     /locus_tag="AC16Y1_06505"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018514306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HIR5832146.1"
                     /translation="DEREQTLNQMLVEMDGFDANVGVILIAATNRPDILDPALLRPGR
                     FDRQVAVESPGLTGRYDILKVHAKGKPIAEDVDLMAVARRTPGFTGADLANVLNEAAI
                     LTARGAAKLITDEILDEAIDRVIAGPQKRTRLMSEQERLITAYHEGGHALVAAALPNT
                     DPVHKVTILPRGRALGYTMVLPETEKYSTTRSQMLDQLAYMLGGRAAEELIFHDPTTG
                     ASNDIEKATAVARKMVTEYGMTERLGAIKYGQATGEPFMGRDFGHQRDYSEEVAAVID
                     EEVRTFIEIAHHEAYEILVANRKVLDALVVELLEKETLDREEVATIFEPLKRRPERPA
                     WTGSARRKPSRKPPVAVPPKIIEEDAAAAETSNA"
BASE COUNT          360 a          274 c          295 g          304 t
ORIGIN      
        1 cgatgaacgc gaacagactc ttaaccaaat gttggttgag atggatggtt tcgatgcaaa
       61 cgtcggcgta attttaattg cggcaacaaa ccgtccggat attcttgatc ctgcgttact
      121 tcgaccagga cgttttgatc gccaagtggc tgttgaatca ccaggcttaa ctggtcgcta
      181 tgacattttg aaagttcacg ctaaaggtaa accaattgcc gaggatgtcg acttaatggc
      241 ggtcgctcgt cgcacacctg gtttcactgg agcagattta gctaacgtgc ttaacgaagc
      301 tgcaattctt acggctcgtg gcgcagctaa gttaatcact gacgaaatac ttgatgaagc
      361 aattgatcga gtaattgctg gtccacaaaa acgtactcgc ttaatgtcag aacaagaacg
      421 tttaattact gcttaccacg aaggcggtca cgcattagtt gcagctgcat tacctaatac
      481 tgatccagta cataaggtga caattctgcc tcgcggtcgc gcacttggtt acacaatggt
      541 gcttcccgaa actgaaaagt attcgaccac tcgttcgcaa atgcttgatc aacttgctta
      601 catgctaggc ggccgcgcag ccgaagaatt aatcttccac gatccaacta ctggtgctag
      661 caatgacatt gaaaaagcaa cagcagtagc tcgcaaaatg gttaccgaat acggcatgac
      721 tgaacgttta ggtgcgatta agtacggaca ggcaacaggt gaaccgttca tgggtcgcga
      781 tttcggtcac caacgcgatt actcagaaga agttgctgca gtaattgatg aagaagttcg
      841 tacatttatt gaaattgcac accacgaggc atatgaaatt ttggttgcta atcgaaaagt
      901 tttagatgca ttagtcgttg agttacttga aaaagaaacg cttgatcgcg aagaggttgc
      961 gacaattttt gaaccactaa agcgtcgacc agaacgtcca gcttggaccg gttcggctag
     1021 acgaaagcca agccgtaaac caccggttgc tgttccgcca aagattattg aagaggatgc
     1081 agcagcagct gagacgagca acgcgtgagt attgatccaa tcgaacaact tcccgagcca
     1141 ccggcattaa agccatacga tgcaaaacgt gttgaaaatg caattcgtga actactaatt
     1201 ggcattggtg aaaatccaga ccgtgaggga ctg
//
LOCUS       DBNHVN010000339         1109 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_213114, whole genome shotgun sequence.
ACCESSION   DBNHVN010000339 DBNHVN010000000
VERSION     DBNHVN010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1109)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_213114"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="AC16Y1_06510"
     CDS             complement(<1..196)
                     /locus_tag="AC16Y1_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HIR5832147.1"
                     /translation="MAKIVSFDEDARRALERGVNALADAVKVTLGPKGRNVVISKSWG
                     APTITNDGVTIAKEIELEDSY"
     gene            complement(321..620)
                     /gene="groES"
                     /locus_tag="AC16Y1_06515"
     CDS             complement(321..620)
                     /gene="groES"
                     /locus_tag="AC16Y1_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR5832148.1"
                     /translation="MSVNIKPLEDRVLVQSLEAEQTTASGLVIPDTAKEKPQEGKVIA
                     VGPGRFDEDGEKRIPLDVKVGDVVIYSKYGGTEVKYAGQEYLLLGARDILAIVEK"
     gene            complement(748..>1109)
                     /locus_tag="AC16Y1_06520"
     CDS             complement(748..>1109)
                     /locus_tag="AC16Y1_06520"
                     /inference="COORDINATES: protein motif:HMM:NF046132.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6912 family protein"
                     /protein_id="HIR5832149.1"
                     /translation="SPGVNLSERVGFALLPVWALGQSDQDPEVLEHELLMLAAQMSLE
                     NGRRLVFVADLPATELDQSLAQVAVPEFSSGQILAMFTDDQANIEAILAGNDGQDLDL
                     TWFGTGEILEILDFIRP"
BASE COUNT          278 a          286 c          229 g          316 t
ORIGIN      
        1 cgtacgaatc ttccagttca atttctttag caatagtcac gccatcgttg gtaattgttg
       61 gtgcgcccca ggacttactg attactacgt tgcgtccttt aggaccaagc gttactttta
      121 ctgcatcagc caaggcgttt acgccacgct ctaatgcgcg acgagcatct tcatcaaaac
      181 taactatctt tgccattact acttcttttc ttgagtttgg ataaaacttt taagaattaa
      241 atagcgcccc gattatcgag gcgctattcg aatttaatat ttacttttcg ttcttttgat
      301 agataaaaaa tgtctagcga ttatttttca actatcgcaa ggatgtcgcg agcgcctaga
      361 agtaaatact cttgtccggc gtacttaact tcggtgccgc cgtactttga gtagatcact
      421 acgtcgccga ctttaacgtc tagcggaata cgcttttcgc catcttcatc gaaacgacct
      481 gggcctactg cgattacttt gccttcttgt ggcttttctt tagcagtatc tggaataacc
      541 aaacctgatg cggtggtctg ctctgcttct aaggactgaa cgaggacgcg atcctcaagt
      601 ggcttaatgt tgaccgacac ggcctgactc ctgttattaa tcgactaatt cctacgaacc
      661 aactggatag ttggttagca cccagaccta tagagtgcta atcccaatct aagagttctt
      721 tggcactcga gcaaatcgag tgccaattta gggccgaata aagtccaaaa tctcaaggat
      781 ttctccggtt ccaaaccagg ttaaatccag gtcttggcca tcatttcccg ccaaaattgc
      841 ctcgatgttt gcctgatcgt ccgtaaacat ggccaaaatc tggccactag agaactcggg
      901 aactgctacc tgggccaaac tttgatccag ctcagttgcg ggcaaatccg cgacaaaaac
      961 taggcggcgc ccattttcca ggctcatttg ggcggccaac attaatagct catgctcgag
     1021 tacctcagga tcctgatctg attgccccag tgcccagacc ggcagcaggg caaaaccaac
     1081 tcgctcgctt aagttaactc ctggacttt
//
LOCUS       DBNHVN010000340         1204 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_213176, whole genome shotgun sequence.
ACCESSION   DBNHVN010000340 DBNHVN010000000
VERSION     DBNHVN010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1204)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1204)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1204
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_213176"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="AC16Y1_06525"
     CDS             <1..534
                     /locus_tag="AC16Y1_06525"
                     /inference="COORDINATES: protein motif:HMM:NF014874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR5832150.1"
                     /translation="AASAVASILGMDLRSIATALDQVTIKSKWRMEVHQLKNSITLIN
                     DAYNANPESMAAALKTLASIETIGRRWAILGTMHELGNEAMTEHDRIGRLVVRLNIDQ
                     LVVVGDTAKALHLGAAQEGSWDGESVWFADFAQACDYICKRVKPNDVLLFKASRSEAF
                     DRLAELVANQINSEGEY"
     gene            537..>1204
                     /locus_tag="AC16Y1_06530"
     CDS             537..>1204
                     /locus_tag="AC16Y1_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HIR5832151.1"
                     /translation="MKLVVLAGALSAILSLVCTPLLIKFLRRRGYAQAIRESADGINY
                     PEHGSKKGTPSMGGAAIIFSVIAGYALAHLISWRQPSVSGLLAIYLMVALGAVGIADD
                     YLKIFKQRSTGIRARTKLLGQAVAAISFGILGQMFPDESGQTPISNAISWVRDTNIVL
                     PSALLMIWIWFLITGITNGVNLTDGLDGLATGAATMAFAAYTVMSIWQYGQSCYWEEI
                     PGCYT"
BASE COUNT          322 a          252 c          296 g          334 t
ORIGIN      
        1 gctgcaagtg ccgtggccag cattttaggt atggacttac gttcaatagc cacagcctta
       61 gatcaggtaa ccatcaaaag taaatggcga atggaagttc atcaattaaa gaactcaatc
      121 acgttaatta atgatgcgta taacgcaaat cctgagtcaa tggctgctgc gctcaagaca
      181 cttgcttcga tcgaaaccat tggtcggcgc tgggcaatat tgggcacgat gcatgaactt
      241 ggcaatgaag cgatgactga acacgatcga atcggtaggt tagttgttcg actgaatatt
      301 gaccaactcg tggtggttgg tgataccgct aaagcacttc acttaggcgc agctcaagaa
      361 ggttcttggg atggtgaatc ggtgtggttc gccgattttg cacaggcttg cgactacatt
      421 tgcaagagag tcaaaccaaa cgatgtgctg ctgtttaaag cctctcggtc ggaagctttt
      481 gatcgacttg ctgaattagt agccaaccaa attaattccg aaggggagta ctaagcgtga
      541 agcttgtcgt cctcgccggg gcactttctg caatattgtc gttagtctgc acaccactat
      601 taattaaatt ccttagacga cggggttacg cacaagctat tcgcgaatct gccgacggga
      661 ttaattaccc agagcatggc agtaaaaaag gcacgccatc aatgggcgga gcagccatta
      721 ttttttcggt tattgctggc tacgcattgg cacatttaat ttcgtggcgc caaccaagtg
      781 tcagtggact tttggcgatc tacttaatgg ttgccttagg tgccgtcggt attgcggatg
      841 attacttaaa gatttttaaa caacgtagca ctggaatccg agctagaaca aagttattgg
      901 gtcaagcagt agctgctatc tcgtttggca ttttgggaca gatgtttccc gacgaaagtg
      961 gtcaaacacc aatttcaaat gcgatctctt gggtacgaga cacaaacata gttttaccgt
     1021 cagcactatt aatgatttgg atttggttct taattaccgg tatcaccaac ggcgttaact
     1081 taactgatgg ccttgacggt ttagcaactg gcgcagccac aatggcattc gctgcatata
     1141 ctgtgatgtc aatttggcag tacggtcaaa gttgttactg ggaagaaatt cctggttgtt
     1201 atac
//
LOCUS       DBNHVN010000341         2710 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_734233, whole genome shotgun sequence.
ACCESSION   DBNHVN010000341 DBNHVN010000000
VERSION     DBNHVN010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_734233"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="AC16Y1_06535"
     CDS             complement(<1..143)
                     /locus_tag="AC16Y1_06535"
                     /inference="COORDINATES: protein motif:HMM:NF014053.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5832152.1"
                     /translation="MSSLPAKVDVVIVGAGLAGLAAARRLTIAGKSVCVVEAADEIGG
                     RVRT"
     gene            complement(157..1035)
                     /locus_tag="AC16Y1_06540"
     CDS             complement(157..1035)
                     /locus_tag="AC16Y1_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018844065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HIR5832153.1"
                     /translation="MKIAIAGSSGLIGTALVESLRADGHEILRLVRRKAVATSEVWWD
                     PTTEQIDLASLNGVEVLINLAGAGVGDRRWTNKYKNLILSSRVQSTKTIATAALTIKP
                     KVLINASAIGYYGDTGDQEVDETSAPGTGFLVDVVKAWEDTTSVASNAGIRVVNLRTG
                     LVVSSKGGAWQRLLPLFKLGLGGRLGNGKQFWSFISIRDEVAAIKFLIENETISGPVN
                     LTAPNPASNREVTKAMAKIFKRPALLNVPATALKIVLGEFSQEVLGSCRVIPRKLMAA
                     GFKFSDPDIVSAIKTL"
     gene            complement(1077..>2710)
                     /gene="sucB"
                     /locus_tag="AC16Y1_06545"
     CDS             complement(1077..>2710)
                     /gene="sucB"
                     /locus_tag="AC16Y1_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HIR5832154.1"
                     /translation="TVTRWLKNVGDTVAADEALLEVSTDKVDTEIPSPVSGVLTEIIA
                     GEDAVVPVGGALGVIAEAGTSASSSPAPVAAAAPTVETPAAPAASAPTPEASAAVTPA
                     AATATTPVLLPALGESVTEGTVTRWLKAIGDTVSADEALLEVSTDKVDTEIPSPVSGV
                     LLEITVPEDGLAKVGGQLGLISSSAGSVAPTPAPSAPAAAPAPASPSPAPPVAPVVQV
                     SNTTIDNSGSYATPIVRKLAAELGVDLSNVKGTGVGGRVRREDILATSPTMAPVKVSS
                     DRPVESKPHVAPTAVSATSTVSPQAALVRGKTEKMTRLRSVIAQRMVESLQISAQLTS
                     VVEVDVTKISVLRNKSKSAFEQREGVKLSFLPFFAKAACEALKQFPTVNASLNQEEGT
                     VTYYDAEHLGIAVDTERGLLVPVIRDAGDLNIAGLARKIDELAQRTRTNKLSPDELSG
                     GTFTITNTGSRGALFDTPIINQPQVAILGTGAVVKRPVVVTDSSGADVIAVRQMVYLS
                     MTYDHRLVDGADAARFLSAIKARLEEGAFEADLGL"
BASE COUNT          699 a          662 c          610 g          739 t
ORIGIN      
        1 gtgcggacgc gcccaccaat ttcatctgct gcctcaacta cgcaaacact ttttcctgca
       61 attgttaatc gacgtgctgc agccaaacct gccagaccgg cgccgacgat aactacatct
      121 actttggcag gcagcgagct cattaaatcc gattcgttaa agtgttttta tagcactaac
      181 gatatcagga tcagaaaatt taaacccagc cgccattaac tttctcggaa tcactcggca
      241 acttcccaaa acttcttggg aaaattcacc taaaactatt tttaatgcag ttgctggaac
      301 atttaaaagt gctggtcgct tgaaaatttt ggccatagct ttggtaactt cacggttact
      361 tgctggattt ggcgcagtta aattaacggg tccagaaatt gtttcgtttt cgattaagaa
      421 tttaattgct gcaacttcat cacgaattga aatgaacgac caaaattgct ttccattacc
      481 aaggcgtcca cctaagccga gtttgaacaa tggcagcaat cgttgccatg ccccaccttt
      541 gctactaacc actaaacctg ttctcaaatt aacgactcgg atgcctgcat tacttgctac
      601 agatgttgtg tcttcccacg ctttgacgac gtcgaccaaa aacccagttc ctggggcaga
      661 agtttcatcc acttcttgat cgcctgtgtc tccgtagtag ccaattgcgc ttgcattgat
      721 caaaactttt ggctttatcg ttagcgctgc ggtagcaatt gtttttgtcg attgcacacg
      781 tgagctaagg attaaatttt tatatttatt tgtccaacgc cgatcgccaa cacctgcacc
      841 agccaaattt ataagcactt caacgccatt aagacttgct aaatcgatct gttcagttgt
      901 tggatcccac caaacttcag aagttgctac agctttacgt cggacaagtc gaagaatttc
      961 atgtccgtcg gcacgcagag attcaaccag ggctgtacca attagtccag atgaaccagc
     1021 aattgcgatc ttcatgtaac tagtttgcag gtgatttgag ttttatgcga gcgcaattac
     1081 agaccaaggt ctgcctcgaa ggcgccttct tcaaggcgag ccttaattgc agacaagaag
     1141 cgagcagcgt cagccccatc aaccaatcgg tgatcgtaag tcatcgacaa ataaaccatc
     1201 tgccgaacgg caattacatc tgcaccactt gaatcagtta ccacaaccgg acgcttaact
     1261 accgcaccgg tgccaagaat ggccacctgc ggttggttaa ttattggtgt gtcaaaaagc
     1321 gctccgcgac tgccggtatt tgtgattgtg aaagtaccac cacttaattc atctggtgaa
     1381 agtttgttag tccgggtgcg ttgggcaagt tcatctattt tgcgcgctaa tccagcgatg
     1441 tttaagtctc cagcatcacg aataaccgga actagtaaac ctcgttcagt gtctacggct
     1501 atgcctaagt gctcagcatc gtaataagtt acagttcctt cttcttgatt cagcgatgca
     1561 ttaacagttg ggaattgctt taatgcttcg caggcggctt ttgcgaagaa tggcaggaag
     1621 gaaagtttta cgccttcacg ttgttcaaac gcagattttg atttattgcg aagtactgag
     1681 attttggtta cgtccacctc tactacagat gtaagttggg ctgaaatctg cagcgattca
     1741 accatccgct gcgcaataac tgaacgcaga cgagtcattt tttcggtctt accgcgaacc
     1801 aaagctgctt gtgggctaac tgtcgaggta gcactaacag ctgttggcgc tacatgaggc
     1861 ttactttcca ctggacgatc tgaggaaact tttactggcg ccatagttgg gctagttgcc
     1921 aaaatgtcct cacggcgaac tcgtccaccg actccagtgc cttttacgtt cgaaaggtct
     1981 acgccaagtt ctgcggctag tttgcgcaca atcggagttg cgtatgaacc tgaattatca
     2041 attgttgtat tcgaaacctg tactaccggt gcaactggag gagcaggtga aggtgaagct
     2101 ggagctggag cggcagcagg ggcacttggc gctggagtag gtgctacaga tcctgctgaa
     2161 ctacttatta aaccaagttg tccgccaact ttggcaagtc catcttcggg aacagttatt
     2221 tcgagtagca cccccgatac tggtgaagga atttcagtat cgactttgtc agttgaaact
     2281 tccaaaagtg cttcatctgc acttactgtg tcaccgattg cttttaacca gcgagttaca
     2341 gttccttcgg taacactctc acccaaggct ggcaataaaa ctggagtggt tgctgttgct
     2401 gcagcaggag ttactgcagc agaagcttct ggggtaggag cgctagcagc aggggctgct
     2461 ggagtttcaa cagtcggagc agctgctgca acaggtgctg gactacttga cgcactagta
     2521 cccgcttcag caataacacc aagtgcgcca ccaactggaa ctactgcgtc ttcaccagca
     2581 ataatttcgg ttagcacacc acttactggt gagggaattt cggtatcaac tttgtcagtt
     2641 gaaacttcaa ggagcgcttc gtcagctgcc acagtgtcgc caacattttt aagccagcga
     2701 gtgactgtac
//
LOCUS       DBNHVN010000342         1283 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_511730, whole genome shotgun sequence.
ACCESSION   DBNHVN010000342 DBNHVN010000000
VERSION     DBNHVN010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_511730"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..152
                     /locus_tag="AC16Y1_06550"
     CDS             <1..152
                     /locus_tag="AC16Y1_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016814709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIR5832155.1"
                     /translation="YDGVLKIDGEVGNKKAYDPRAYGKLAEANMAARVVKACEDLRST
                     GTRIK"
     gene            224..>1283
                     /locus_tag="AC16Y1_06555"
     CDS             224..>1283
                     /locus_tag="AC16Y1_06555"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003975310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR5832156.1"
                     /translation="MPAIVLVGAQWGDEGKGKATDLLGGRVDYVVRYQGGNNAGHTVV
                     IGDKKFALHLLPSGILTPEVTPVIGNGVVIDAGVLFTEIEGLEKQGIDTSKLVVSANA
                     HLITSYHVTLDKVTERFLGNSKIGTTGRGIGPTYADKISRVGIRVQDLFDEKILRAKV
                     EGALNNKNQVLVKVFNRRAITANEVVEELLSYADRLRPFVADTALLLNNALDQNKVVL
                     LEGGQGTLLDVDHGTYPFVTSSNPTAGGACAGSGIGPTKITGVIGILKAYTTRVGSGP
                     FPTELLDEDGEKLRTIGGERGVTTGRPRRCGWFDAPIARYATRVNGLTDIFLTKLDVL
                     TGWEKIPVCVAYDIDGKRS"
BASE COUNT          367 a          244 c          313 g          359 t
ORIGIN      
        1 attatgacgg cgtgctaaaa attgatggcg aagttggcaa taagaaagcc tatgatccgc
       61 gcgcttatgg taagttggcc gaggcaaata tggctgctcg ggtggttaaa gcgtgtgagg
      121 atttacgctc aactggcact cggataaagt aagtgcgggt ttgaacttct ctgcaaatct
      181 gtcaatttaa tttattccgt aaatttaata aaaaaggaga gccatgcctg ccatcgtgct
      241 agttggcgcc caatggggtg acgaaggaaa aggtaaagct accgatttac tcggtggtcg
      301 agtcgattat gtagttcgtt atcaaggtgg aaataacgct ggtcacactg ttgtgatcgg
      361 tgacaagaaa tttgccttgc accttttacc aagtggaatc ctcacaccag aagttactcc
      421 agtgattggt aacggagttg tgatcgatgc tggtgtactt ttcaccgaaa ttgaaggact
      481 tgaaaaacaa ggtatagata cttcaaagtt agttgtaagt gcgaacgcgc atttaattac
      541 ttcttatcac gtcactttag ataaagttac tgaacgcttt ttaggtaatt caaaaatcgg
      601 aactaccggt cgaggtattg gcccaaccta cgcagataaa atttcgcgtg ttggtattcg
      661 agtgcaagat ttgtttgacg aaaagatttt gcgtgccaaa gttgaaggtg cattaaataa
      721 caaaaaccag gtcttggtaa aagtttttaa tcgtcgtgcg attacagcaa acgaagttgt
      781 tgaagagtta ctttcttacg ctgatcggct aagaccattt gtagctgata ctgcactatt
      841 acttaacaat gcacttgatc aaaataaagt tgttttactt gaaggtggcc agggaacact
      901 acttgatgtt gatcacggaa cttatccatt cgtaacctca agtaatccaa cagctggtgg
      961 cgcttgtgct ggttcaggta ttggcccaac caaaatcacc ggcgtgattg gcatccttaa
     1021 ggcttacaca actcgagttg ggtcaggacc gttcccgact gagttactag atgaagatgg
     1081 cgaaaagctt cgaacaatcg gtggcgaacg tggcgtaaca acgggacgcc cacgacgctg
     1141 cggttggttt gacgcaccaa ttgctcgtta tgcaacacgt gttaatggtc taaccgatat
     1201 tttcttaacc aaacttgatg tattaactgg ctgggaaaaa attccagttt gtgttgcgta
     1261 tgacattgat ggcaaacgaa gcg
//
LOCUS       DBNHVN010000343         4189 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1255684, whole genome shotgun sequence.
ACCESSION   DBNHVN010000343 DBNHVN010000000
VERSION     DBNHVN010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1255684"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="AC16Y1_06560"
     CDS             complement(<1..569)
                     /locus_tag="AC16Y1_06560"
                     /inference="COORDINATES: protein motif:HMM:NF016365.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HIR5832157.1"
                     /translation="MFTGAGTVINLGTIVLGTFIGVFLAHRLADRVRNVVTDGLGLIT
                     LISGFIAVLDLMKPEFKAALPDGSSLLVPLGAILLGGITGSFLRIEDRLNNFGDNLRK
                     RFAKADSSSKFTEGFVAASLLFGIGPLALMGSISDGLGNGIDQLLLKSTLDFFASIAF
                     AASFGWGVAASIIPVGLLQGTFTVLGMYLG"
     gene            complement(580..987)
                     /locus_tag="AC16Y1_06565"
     CDS             complement(580..987)
                     /locus_tag="AC16Y1_06565"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR5832158.1"
                     /translation="MSESVDTSLIPVGPMAERMGIKLLSASSQEVIGTMPVEGNTQPF
                     GLLNGGASMALVETLGSIGAVIHAGRERIAVGIEINGTHHKSARTGLVIGKATAISLG
                     NTLATYEVKIFDETETLICTGRITCLIRDRKAK"
     gene            1012..3711
                     /gene="polA"
                     /locus_tag="AC16Y1_06570"
     CDS             1012..3711
                     /gene="polA"
                     /locus_tag="AC16Y1_06570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR5832159.1"
                     /translation="MANTSKFLILDGHSLAYRAFHGLPVENFVTSSGQHTNAVYGFTS
                     MLLNVLKEQKPTHLAVAFDVSRDTFRSKRYPDYKATRAKTPPEFKGQVELIKEILVAL
                     NIKVVTADGYEADDILATLAHACDFKVNIISGDRDTFQLVDDRVTVLYPRKGMSDLVL
                     MTPEAVQEKYGVNPSQYRDLAAMVGEASDNLPGVPGVGPKTGAKWIVQYGSLGEIIAK
                     SDEITGKVGESLRANIDRAKENYDLNRLVTDVPIDTDISLYQKQDYQPNVVHEVFDAI
                     EFSALRSRLLSAWPTKLSAGVPAQAEAKGKNSESKVEAKASSSVAGKKVDAKELATWL
                     KEQNGKDVAVAFSGQWGQGAGNIDTVALLSDSGDGIWVEFALAKNELKTWFESAASKI
                     IHDAKGPALALLNSEIKVNNIKMDTALAAYLLAPGGRNFSLPDLALRYLKKDLTEVSA
                     QPAQLSFETNAQADGQALIIHAEATMQLAKYFETELATRGVAKLLTDMEIPLIHVLVE
                     MESAGIAIDVSSLKKLEDSFEADVQSCIKQAHKAAGHEFNLGSPKQLQEVLFEERGLP
                     KTKKIKTGYTTDADALQGLLERFPEDELLVALIRFREISKLRQTVTGLLSCVANDGRI
                     HTTFNQMIAATGRLSSTEPNLQNIPIRTNEGGLIRDTFVVGKGYETLLTADYSQIELR
                     VMAHLSNDQGLIEALKSGEDLHTTVGSYVFGVAKDKVDAEMRRKVKAMSYGLAYGLSA
                     FGLANQLGIGTGEAKTLMDKYFERFGGVREYLAKVVQEARKVGYTESIFGRRRYLPDL
                     NSDNRQLRDIAERAALNAPIQGSAADLVKIAMLNVDRALKDAGVKSRLLLQVHDELII
                     EIAKGELEQVSSLVRENMANVANLSVPMEVSLGVGQSWAKAAH"
     gene            3914..>4189
                     /locus_tag="AC16Y1_06575"
     CDS             3914..>4189
                     /locus_tag="AC16Y1_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018191155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR5832160.1"
                     /translation="MTVTTPKSQSITVNDIGSAEDFLAAIDKTIKPFDDGDLVEGIVV
                     KVDRDEVLLDIGYKTEGIIPSRELSIKHDIDPNEVVKVGDTIEALVLQ"
BASE COUNT         1261 a          890 c          947 g         1091 t
ORIGIN      
        1 cctagataca ttcctagaac agtaaaagtt ccttgcagca ggccaacggg gattattgat
       61 gcagcaacgc cccagccaaa tgatgcagca aaagcaatgg aagcaaaaaa gtccaaagtt
      121 gatttcaaca gcagttgatc aattccatta cctaagccat ctgaaatcga acccattaat
      181 gccagcggcc cgatgccaaa aagtagggaa gccgcaacaa aaccttcggt aaatttgctc
      241 gacgaatctg ctttggcaaa acgcttgcgt aaattatcac caaagttatt aagtcgatct
      301 tcgatgcgaa gaaatgatcc agtgattccg ccaagcaaaa ttgcgccgag aggaactaaa
      361 agcgaactac catccggaag tgcagccttg aattccggtt tcattaaatc cagcacggcg
      421 atgaaaccac taattaaggt gattaaacca agaccatcag taactacgtt gcgaactcga
      481 tcggctaacc gatgcgcaag aaatacacca ataaaggtgc caagcacaat tgtgccaagg
      541 ttaattacag ttcctgcacc cgtgaacatt aactaatacc tacttagctt tgcgatcgcg
      601 aatgaggcaa gttattcgac cggtacaaat tagcgtctcg gtttcatcaa aaatcttcac
      661 ttcgtaagta gccaaagtat tacctaagct aattgctgtt gccttaccga ttactaaacc
      721 cgtgcgtgca gatttatggt gggtgccatt gatttcgatt ccaacagcaa ttcgttcccg
      781 gccagcatgt attactgcac cgatagaacc caaagtttca actaaggcca tcgaagcgcc
      841 gccgttaagt aggccaaatg gttgagtgtt tccttcaact ggcatagttc cgattacttc
      901 ttggctactt gcgcttaaca atttaatgcc catccgttcg gccattgggc caactgggat
      961 aagtgatgtg tcaacggatt cagacattta ggagccctct aggatcaaag tgtggcgaat
     1021 acctctaagt ttctcattct tgatgggcac tcgctggcat accgcgcctt tcacggctta
     1081 cctgtagaga attttgtcac gagttcgggc caacatacta atgcggtcta tgggttcact
     1141 tcgatgttgt taaacgtctt aaaagaacaa aagccaaccc atctagccgt ggcatttgat
     1201 gtctctcgag acacattccg ttcaaagcgt taccccgatt acaaagccac tagggcaaag
     1261 acaccaccag aatttaaagg ccaagttgag ttaattaagg aaattttggt cgcgctgaac
     1321 ataaaagttg ttactgcaga tggctacgag gccgatgaca tcttggcaac actggcacac
     1381 gcttgtgact tcaaagtaaa tataatttcc ggagatcgcg acactttcca gctggtcgac
     1441 gatcgagtta ccgtgcttta cccgcgcaaa ggtatgtcag atcttgtttt aatgactccc
     1501 gaagctgtgc aagaaaaata tggagttaat ccaagtcaat atcgtgattt agctgccatg
     1561 gttggtgagg ctagcgataa tttgcctggc gtcccaggag ttggaccgaa aacaggtgca
     1621 aagtggattg tgcagtatgg ctcacttggc gaaattattg caaagtctga tgaaataaca
     1681 ggcaaagtag gcgagtcact tcgagccaac attgatcgag ctaaagaaaa ctatgattta
     1741 aatcgcttag ttactgatgt gccaatcgat acagacatca gcctttatca aaaacaggat
     1801 taccaaccaa atgttgtgca tgaggtcttt gatgctatcg agttttcggc actacgttcc
     1861 cgattgcttt cggcatggcc cacaaagtta agtgccggcg ttccggcgca agcagaggcc
     1921 aaaggcaaga attcagaatc aaaagttgag gcaaaggcat cctcatcggt agccggtaaa
     1981 aaagttgatg ccaaagaatt agcaacttgg ttaaaagagc aaaacggtaa agatgttgca
     2041 gttgcatttt ctggtcaatg gggtcaaggt gctggaaaca ttgacactgt tgcattactt
     2101 agcgattctg gcgacggtat ttgggttgag tttgcgttag ccaagaacga attaaaaact
     2161 tggtttgaat cagcagcatc aaaaattatc catgacgcaa aaggtccggc acttgctctt
     2221 ttaaatagtg aaataaaagt aaacaacata aaaatggaca cggcgttagc agcatatttg
     2281 ctcgcacccg ggggacgtaa tttctcatta cctgatctgg cgcttcgcta tttaaagaaa
     2341 gatttaacgg aagtttcggc acagcctgct caacttagtt ttgaaacgaa tgcgcaagca
     2401 gacggtcaag cactaataat tcacgcagaa gcaaccatgc aattagctaa gtattttgaa
     2461 actgagttag cgactcgggg agttgccaag cttttaactg acatggaaat cccgttaatc
     2521 catgttctag ttgagatgga atccgctggt atcgcaattg atgtatcaag tttgaagaaa
     2581 cttgaagatt cattcgaggc agatgttcag agttgcataa agcaagcaca caaagctgcc
     2641 ggtcatgaat tcaaccttgg ttcaccgaag caacttcagg aagttctttt tgaagaacgc
     2701 gggctaccga aaactaaaaa aatcaaaact gggtacacca cagatgctga tgcgcttcaa
     2761 ggtttacttg agcgctttcc cgaagatgag ttattggttg cgttaatcag attccgcgaa
     2821 ataagtaaat tacgccagac cgtcaccggt ttactttcgt gtgtggctaa cgatggtcga
     2881 attcacacca cttttaatca aatgattgcc gcaactggtc gactaagtag tactgaaccg
     2941 aatctacaaa acattcctat tcgcactaac gaaggcggtt taatccgcga cacattcgta
     3001 gttggtaaag gttatgaaac tttactaact gctgattaca gccagattga attgcgtgtt
     3061 atggcgcact tgtctaatga tcaaggctta attgaggcac tcaagtctgg tgaagaccta
     3121 cacaccacag ttggttccta tgtatttggt gtggccaaag ataaagtgga tgcagaaatg
     3181 cgcagaaaag ttaaagccat gtcatatggg ttggcttatg gtctatctgc atttggcttg
     3241 gctaatcaac ttggcatagg tacgggtgaa gccaagactc tgatggataa atattttgaa
     3301 cgatttggtg gcgtgcgtga atatttagct aaggtcgttc aagaagctcg caaagttggt
     3361 tataccgagt cgatatttgg tcgccgtcgc tatttacccg atttgaatag tgacaatcgg
     3421 cagttaaggg atattgctga acgtgctgca ctcaatgcgc cgattcaagg aagcgcagct
     3481 gacctggtga agatagcaat gttaaatgtt gatcgtgcac ttaaagacgc tggcgtaaaa
     3541 agtcggttgt tacttcaagt tcatgatgaa ttgataatcg agattgcaaa gggcgaactt
     3601 gagcaggtca gcagcctagt ccgcgaaaat atggcaaacg tggccaacct cagtgtgccg
     3661 atggaagtat cacttggtgt tggacaaagt tgggctaaag cagcgcacta attggttcga
     3721 tcctcaggta tttcgtggat taaatctggg gaaaattgcc tatttagagc gctttaccta
     3781 ttcttagaag gttgaaattc gccatttttt agacctagaa agaatggctc aactaatcaa
     3841 actaaaaacc tggttatttg aaaaagtgat ttcagcatcc gcactataac tatccactaa
     3901 aggcattaac ttaatgaccg ttactacacc taagagtcaa tcaatcactg taaacgacat
     3961 tggctctgcc gaggatttcc tcgccgcaat tgataagacc atcaaacctt tcgacgacgg
     4021 agatctcgtt gagggcatcg ttgtaaaagt agaccgtgac gaagttcttt tggacatcgg
     4081 ctacaaaact gaaggcatca ttccttctcg cgaactaagt atcaaacacg acattgatcc
     4141 aaatgaagtc gtaaaagttg gcgacacaat cgaagcttta gttcttcaa
//
LOCUS       DBNHVN010000344         4246 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1329754, whole genome shotgun sequence.
ACCESSION   DBNHVN010000344 DBNHVN010000000
VERSION     DBNHVN010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1329754"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /gene="tsaE"
                     /locus_tag="AC16Y1_06580"
     CDS             <1..471
                     /gene="tsaE"
                     /locus_tag="AC16Y1_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010986375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR5832161.1"
                     /translation="IISDADQMHKFGYAVGKQLHAGDYVVLTGNLGAGKTTFTQGLAA
                     GLEVIGSVTSPTFVISRIHKPAGDGPKLVHVDAYRLTGNDQLLDLDLADQINSVVVIE
                     WGAPYISELTDSWLAIDIARESDVDNTDPAAGIRTANLTAFGDRWRDFNLAELQ"
     gene            468..1085
                     /gene="tsaB"
                     /locus_tag="AC16Y1_06585"
     CDS             468..1085
                     /gene="tsaB"
                     /locus_tag="AC16Y1_06585"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR5832162.1"
                     /translation="MKILAIDTSVGVSVAILENQNILAQFTDISHGIQGELTSAKISQ
                     LLKQTDLSSNDIDKVLVGVGPGPFTGLRVGIATAQAFSFALNIPHFGICSLDAVAYEF
                     GKPCVVVTDARRKELYWARYEKNKIAGPAVATPAELVALQADCIFIGPAAALYPEFIN
                     GEVMPLTAGALGQLYASGEAELLPARPLYLRKPDAQESTVRKSVL"
     gene            1085..1528
                     /gene="rimI"
                     /locus_tag="AC16Y1_06590"
     CDS             1085..1528
                     /gene="rimI"
                     /locus_tag="AC16Y1_06590"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR5832163.1"
                     /translation="MQIREMKNSDLDQVIEIEQKLFPTDAWTISLFLEELAQVPISRS
                     VVVGQIGQEIIGYASLRFVGKEGDINTIAIAPSHQRKGYGNLLLTWLLENAKAKGVRE
                     LFLDVRADNAAAIEMYKNAGFERIDIRRNYYDNSVDAHVMRKKIS"
     gene            1525..2571
                     /gene="tsaD"
                     /locus_tag="AC16Y1_06595"
     CDS             1525..2571
                     /gene="tsaD"
                     /locus_tag="AC16Y1_06595"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR5832164.1"
                     /translation="MSEPLVLGIETSCDETGVGIVRGTTLLADEVATSVDEHVRFGGV
                     VPEVASRAHLESIVPTIERACATAKIKVSDIDAIAVTAGPGLAGALLVGVSSAKALAV
                     GLNKPIFGVNHLAAHVCVDQLEHGYLDNPSVALLVSGGHSSLLLVPEISGEVIPLGQT
                     IDDAAGEAFDKVARVLGLPFPGGPYIDQAAKEGNRAAIEFPRGLTSTKDLAKHRFDVS
                     FSGLKTAVARWVELETKKGNQIMVNDVAASFQEAVVDVLLRKAIDACLVNEVSTLVIG
                     GGVAANSRLRQVAQERCTENKIKLRIPRPGLCTDNGAMVAALGSELVRQGKFPSPIEF
                     GTDSAMDVRTVVER"
     gene            2571..3485
                     /gene="mmuM"
                     /locus_tag="AC16Y1_06600"
     CDS             2571..3485
                     /gene="mmuM"
                     /locus_tag="AC16Y1_06600"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF007020.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047150 - betaine-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase"
                     /protein_id="HIR5832165.1"
                     /translation="MQSFNDFLPAGPWAIDGGLSGELEKRGHDLSDKLWSARLLRDEP
                     EAIAHVHQDYVAAGARVIITASYQASRKGFVEFGLTERKADELIELSVTLAKQVVSQA
                     KDKVWVAASVGPYGAVLGGGQEYVGNYGVSHKDLVEFHTQRIEVLAKADPDLFACETI
                     PDIAEVEALLEVLKKYPNIPAWITMSAKNGTQSCAGQDITQLAKAVEKFPNVVAIGVN
                     CTMPEFVTSLFERLSAATDLPLVVYPNAGRVWDGQGRCWIGEGHSTLPTQIISEWVKL
                     GAKLIGGCCGLGPDAISSIDQDLKPYRN"
     gene            complement(3487..3975)
                     /locus_tag="AC16Y1_06605"
     CDS             complement(3487..3975)
                     /locus_tag="AC16Y1_06605"
                     /inference="COORDINATES: protein motif:HMM:NF022161.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HIR5832166.1"
                     /translation="MPAELNFDFELPASAKATFEMLTHPDFLTEKIALAQSGKYSISG
                     DNPNLVVDITRTVTADLPDLVRKFVGENLVVQEIQNWKEVSTNNYLADFKLKISNAPV
                     DISGQINLQETQNTKVSITGKVKVNIPLFGATAEPKVVETIMRVLGDEAKLCKDWITK
                     TK"
BASE COUNT         1166 a          838 c         1005 g         1237 t
ORIGIN      
        1 ataatttccg atgcagatca aatgcacaaa tttggttatg cagttggcaa gcagttgcat
       61 gcaggtgact atgttgtttt aactggaaat ctaggtgctg gtaaaacaac ttttactcaa
      121 ggtttagctg caggtcttga ggtcattgga tcagttacta gcccgacatt tgttatttcc
      181 agaattcata aaccagctgg cgatggaccc aaattagtcc acgtggatgc ctatcgatta
      241 actggaaacg atcaattgtt agatctggac ttggcagatc aaattaatag cgttgtggtt
      301 atcgaatggg gcgcccccta catttcagaa cttacagact cctggctggc gattgatatt
      361 gctcgagagt ctgatgttga taacactgat ccagctgcgg gaattcggac cgcgaatctc
      421 actgctttcg gagacagatg gcgtgatttc aacttggcgg aattgcaatg aaaattcttg
      481 cgattgatac ttcggtgggt gtctcagttg ccatccttga aaaccaaaat attctggcgc
      541 aatttactga catttcacat ggaatccaag gtgaacttac ctcagcaaaa atttctcaat
      601 tattaaagca aactgactta agttcaaatg acatcgacaa agttttggtt ggagttggtc
      661 ctggtccatt taccggactt cgcgttggaa ttgctacggc tcaggcattc tcatttgcac
      721 ttaacattcc gcactttgga atttgttcac tggatgccgt tgcgtatgaa tttgggaagc
      781 catgtgtcgt tgtaactgac gcaaggcgta aagaactcta ctgggcgagg tacgaaaaaa
      841 ataaaatcgc tggaccagca gttgccactc cggcagagct agtagcgctg caggcagatt
      901 gcatatttat cggaccagca gcggcactct acccagaatt tattaacgga gaagtaatgc
      961 cacttactgc aggtgctctt ggccaactct atgcatccgg tgaagccgaa cttttgccag
     1021 cgaggccgct gtatttacgt aaaccagatg ctcaagaatc aaccgtacga aagtcggttt
     1081 tgtaatgcag attcgtgaga tgaaaaatag cgatttagac caagttattg aaattgaaca
     1141 aaaacttttt cctacggatg cgtggacaat ttccttattt cttgaagagt tagcccaagt
     1201 accaatttct cgctcagttg ttgttggaca gattggccaa gagattatcg gctacgcctc
     1261 acttagattt gtgggcaaag aaggcgacat caatacgatt gcgattgcac caagtcatca
     1321 aagaaaaggt tacggtaact tactgcttac ctggttacta gaaaatgcaa aagctaaagg
     1381 tgtgcgcgag ttatttttag atgtccgagc cgataatgct gcggcaatcg aaatgtacaa
     1441 gaatgctggg tttgaaagaa ttgatattcg gcgcaactat tacgacaatt cagttgatgc
     1501 tcatgttatg cgaaagaaaa tatcatgagt gaacccttag tccttggcat tgaaacttcc
     1561 tgtgatgaaa caggtgtagg catagtacgt ggaacgacat tattagctga tgaagtagcc
     1621 acaagtgttg atgaacacgt gcgttttggt ggcgtagttc cagaagttgc tagtcgtgcg
     1681 catttagagt ccatagtgcc aactattgag cgagcatgtg ccacagcaaa aattaaagtc
     1741 agtgatattg atgcaattgc cgtgaccgca ggcccaggac ttgcaggcgc acttttagtt
     1801 ggcgtttcaa gcgcaaaagc actcgcagtt ggtctaaata aacctatttt tggtgtgaat
     1861 catttagcgg cgcatgtctg tgttgatcaa ctagagcatg ggtatttaga taacccaagt
     1921 gtggccttac ttgtttcggg cggacattcg tcattattgc tggtgccaga aatatctggc
     1981 gaagtgattc cacttggcca aacgatcgac gatgcagcag gcgaagcttt tgacaaagtt
     2041 gctcgagttt tgggattacc ttttcctggc ggcccctaca tagatcaagc cgctaaagag
     2101 ggaaatcgag ctgcgattga atttccacgt ggccttactt caactaagga cctggccaaa
     2161 catagattcg atgtttcatt ttctggatta aaaaccgctg tggctagatg ggttgagctg
     2221 gaaactaaaa aaggtaatca gatcatggtt aacgatgtcg cagcctcatt tcaagaagca
     2281 gttgtcgacg tactgcttcg taaagcaatt gatgcctgtt tagttaatga agtttcaact
     2341 ttagtaattg gcggtggagt agcggcaaat agccgattgc gccaagttgc acaagaacga
     2401 tgcaccgaaa ataaaatcaa attacgcatc ccgcgcccgg gcctatgcac agataacggg
     2461 gctatggttg ctgcattagg tagtgaatta gttcgacaag gtaagttccc gtcaccgata
     2521 gagtttggga cagatagtgc aatggatgta agaacggttg tagagcggta atgcaaagct
     2581 ttaacgattt tttaccagct ggaccttggg ctattgatgg tgggctttca ggtgaactag
     2641 aaaagcgtgg ccatgatctt tcggacaagc tttggtctgc tcgcttactt cgcgatgaac
     2701 ctgaagctat tgcgcacgtt catcaagatt acgtagctgc tggagcgcgc gtaataatta
     2761 ccgcttctta tcaagcaagc cgaaaaggtt ttgtcgaatt tggtttaact gagcgtaagg
     2821 ccgatgaatt aattgagctt tctgtgactt tggctaagca agtagtaagt caagctaaag
     2881 ataaagtttg ggtggcagcc agtgttggtc cctacggtgc tgtgcttggt ggtggacaag
     2941 aatatgtcgg aaattatgga gtttcacaca aagatttagt tgagtttcat acgcagcgta
     3001 ttgaagtttt agccaaagca gatcctgatc ttttcgcatg cgaaaccatt cctgacattg
     3061 ctgaggtcga agcgttactt gaagtattga aaaaatatcc gaacattcca gcttggataa
     3121 ctatgagcgc aaaaaacggt acccaaagtt gcgctggaca agatatcact caactagcta
     3181 aagctgttga gaagtttcca aatgtagtag cgattggtgt taactgcaca atgcctgaat
     3241 ttgtgacttc actgtttgag cgcctatcag ccgctactga tttgccgcta gtcgtctatc
     3301 cgaatgctgg tcgagtttgg gatggtcaag gtcgatgctg gattggcgag ggccactcaa
     3361 ctttgccgac gcaaattatt tctgaatggg tgaaactagg cgcaaaatta attggtggct
     3421 gctgtggctt aggtccggat gctatcagtt caattgatca agacttgaaa ccgtatcgaa
     3481 attaaatcat ttagttttag taatccaatc tttacacaat ttggcttcgt cgcctagtac
     3541 tcgcattatt gtctcaacaa cttttggttc ggcagtagca ccaaataaag gtatgttaac
     3601 ttttactttt ccagttattg aaacttttgt attttgagtt tcttgcaaat taatttgccc
     3661 agagatatca accggcgcgt tagatatttt caatttaaag tcggcaaggt aattattggt
     3721 cgatacttct ttccaatttt ggatttcttg cacaactaaa ttctcgccaa caaattttct
     3781 aactaggtcg ggcaaatccg ctgtaactgt tctagtgata tcgacaacca gatttggatt
     3841 gtcaccagaa attgaatatt ttccagattg cgccaatgca attttctcgg tcaaaaaatc
     3901 tggatgtgtc agcatttcaa atgtggcttt tgcgctagcc ggcaactcga agtcaaagtt
     3961 cagttcggca ggcatagttc catattgcac taataactga gtaacacttc ggttaaatta
     4021 gccttctagt gcttggttat taagtcgatt ccttcattgc taggcttaat tttaattcac
     4081 taaataaacg gtgacctggg aggcttgcgg tcttgtctga aatgacatcg cgcggatctg
     4141 ttcgtcttga agatgcaaga cgagatcttt tgacccaagt tgcaaatctt gtgccaaagg
     4201 aattttcggc cgttgtcgat gattattttg atttagttgc accgat
//
LOCUS       DBNHVN010000345         3992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1849988, whole genome shotgun sequence.
ACCESSION   DBNHVN010000345 DBNHVN010000000
VERSION     DBNHVN010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3992
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1849988"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="AC16Y1_06610"
     CDS             <1..779
                     /locus_tag="AC16Y1_06610"
                     /inference="COORDINATES: protein motif:HMM:NF019883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5832167.1"
                     /translation="RLQNGQEAVGQLIASKIPLFEFVIKTTMAEFDLATAEGRVGAMR
                     AVTPIVAGIKDSALRIEYTRTVAGWLGIDEATLRKDVSGKSRPGANQHAEAKKTDSRA
                     AAIEREALKCVLQWPALVGNWFESLEESLFTVPMAAEVFQTCVQAGDPLEFSDASKWV
                     QAVLAVAPSDEVKAQIRALAIEPLPNDNPDKKYIQAVLARILEMDASRRVEQLKSQLA
                     LAEVEYDSAEQDRLLAELLSLESYRRSMRDFAIGDQQFEV"
     gene            779..1258
                     /locus_tag="AC16Y1_06615"
     CDS             779..1258
                     /locus_tag="AC16Y1_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5832168.1"
                     /translation="MKLFRKSIPEQLAENLVKNERVLAWANHPGGVVAVTNFALISLD
                     HHERVRIPWELTISGKWDEPLLVVTLQENLGGVPTQRAWNLAQPGLVPTAVRERITTA
                     QVFDQVKEVPEVGIVRFIARQGPQGISWDYLTEEKISADSQTHIENALSQLRQTLGI"
     gene            1304..1376
                     /locus_tag="AC16Y1_06620"
     tRNA            1304..1376
                     /locus_tag="AC16Y1_06620"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1337..1339,aa:Asn,seq:gtt)
     gene            1431..2312
                     /locus_tag="AC16Y1_06625"
     CDS             1431..2312
                     /locus_tag="AC16Y1_06625"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013357.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HIR5832169.1"
                     /translation="MNIRRGISFLLGATLLLIGLGQPAFAKEKSIFGTSAISAQYKSC
                     SYPKGPAVVRPPNAASWSEQGFAEVVKGIDVSRWNHPNNRAIDFKKVKEKYGVTFVII
                     KSSDGGDKGNALAKKWFPVDRKAAKAQGLVVGGYHYALPGNMSKDRTKDAKLQAARAV
                     RQANGAKVGDLPLTLDMEELPCGWTNEQLARWTDVFLKEVKRLTGRTPMIYMNGIFIK
                     RIVGANVADFSEYPLWLAKWGPSLGTNPGDASIWPGKWVIWQFTADGKVSAVPSKTTD
                     LNVFNGTQADFQQFINR"
     gene            complement(2309..3010)
                     /gene="deoD"
                     /locus_tag="AC16Y1_06630"
     CDS             complement(2309..3010)
                     /gene="deoD"
                     /locus_tag="AC16Y1_06630"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013967846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR5832170.1"
                     /translation="MTTPHIGANKGDIADTILLPGDPLRAQWIAENFLENPVKYNSVR
                     NMFGFTGTYKGNRVSVQGTGMGVPSLSIYVTELFKDFDVQTAIRVGTCGAVGPLKLRD
                     VVIAMSASTDSGVNRRATGGLDFAPTANYDLLEAAVRAAKSRNVSHHVGGIATMDVFY
                     DDSNALDVLENLGVYAVEMETSGLYTIAAKYRRRALTICTVSDLIRTHEQTTPAERET
                     GFRAMVEVALDAAFA"
     gene            complement(3076..3654)
                     /locus_tag="AC16Y1_06635"
     CDS             complement(3076..3654)
                     /locus_tag="AC16Y1_06635"
                     /inference="COORDINATES: protein motif:HMM:NF025580.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HIR5832171.1"
                     /translation="MDRSIAISKSALSSATALLAAQWIVALASIPSAQDYANQIAEGK
                     SPEEITTIYDNVSILIFIFSIWSYIATTRLLAFAYDREIKTNPTAVRLKRIWIYLGWV
                     TPVVSFWFPKRIVDDLVEAKSKRINEPNPTSKNSITWWTTWISHDLINAIGFAGAALG
                     TMTRIEPTYQLAAAAMLTASYLVWTKILKALA"
     gene            3706..>3992
                     /locus_tag="AC16Y1_06640"
     CDS             3706..>3992
                     /locus_tag="AC16Y1_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GmrSD restriction endonuclease domain-containing
                     protein"
                     /protein_id="HIR5832172.1"
                     /translation="MKKSLWPVLVVVALVGFCAPTSSDTSLNSTPQVAQNAQTTTAQP
                     SQSSPTQNSSVEDSAPIPSTQAAGGTSRVLIQLTELAIKGRAPKTGYDRDL"
BASE COUNT         1118 a          854 c          966 g         1054 t
ORIGIN      
        1 ttcgcctgca aaatggccaa gaagcagttg ggcaattaat tgcaagtaaa attccacttt
       61 ttgaatttgt gattaaaaca acgatggccg agtttgattt agcaactgcc gaaggtcgag
      121 ttggtgcaat gagggcggta actccgattg tggccgggat taaagatagt gcgctgcgta
      181 ttgaatacac caggacagta gctggttggt taggaattga tgaagcaacg ctgcgcaaag
      241 acgtatctgg aaagtcgcgt ccaggtgcca atcagcatgc tgaggccaag aaaactgatt
      301 cccgagcggc agcgattgaa cgtgaggcac tcaaatgcgt actacagtgg ccggccttag
      361 ttggaaattg gtttgaatca ttagaagagt cactatttac ggttccgatg gcagcagagg
      421 tttttcaaac ctgcgtccaa gccggcgacc cgcttgagtt tagcgatgca agtaaatggg
      481 ttcaggcggt acttgccgtt gcgccaagtg atgaggttaa agcccaaatt cgcgcattag
      541 cgattgaacc tttgccaaat gacaatccgg ataaaaaata cattcaagca gttttagctc
      601 gcatcctaga aatggacgcc agtcgacgag tcgagcaact taagagccag ctggctttag
      661 cggaagttga atatgattcg gctgaacaag atcgactctt ggctgaatta ttgtcgttag
      721 aaagttatcg acgaagtatg cgtgattttg caatcggcga tcagcagttc gaggtttaat
      781 gaaacttttt cgcaaatcaa ttccagagca acttgctgag aatctagtaa aaaatgaacg
      841 cgtgttagct tgggcaaatc atccaggtgg tgtagtcgcc gtaacgaact ttgcacttat
      901 cagcctggat catcatgagc gagtacgtat tccttgggaa ctaacgataa gtggcaaatg
      961 ggatgaaccc cttttggttg tgactttgca agaaaatctt ggtggcgtgc ccacccaacg
     1021 ggcttggaat ttagcgcaac caggtttggt gccaaccgct gttcgtgagc gaataacaac
     1081 tgcccaagtt tttgaccaag ttaaagaagt acctgaggtt ggaattgtgc ggtttattgc
     1141 gcgccaagga ccacagggga ttagttggga ttatctaact gaagaaaaaa taagtgcaga
     1201 ttcacaaact cacattgaaa atgcactcag ccaattgcgc caaaccctgg gcatttagtg
     1261 agtttggggc aatctaacgc ctttgttagt attggtgagc caatcccctg tagctcaatt
     1321 ggcagagcac tcgactgtta atcgagtggt tgttggttcg agtccaaccg ggggagcagt
     1381 taaaccggaa attttgaact acatctagca caggtttagt gggttgaaat ttgaatatca
     1441 ggcgaggtat ctccttccta ctcggcgcga ctttattact cattggtttg ggccaacctg
     1501 catttgcaaa agaaaaatca attttcggca catcagccat cagcgcacaa tacaagtcct
     1561 gcagttatcc aaaagggcca gcagtggttc gaccgccaaa tgctgcaagt tggagcgagc
     1621 aaggattcgc cgaagttgta aaaggtatag atgtttctcg ctggaatcac ccaaacaata
     1681 gagcaattga ttttaagaaa gtaaaagaaa aatatggcgt cacttttgta atcataaaat
     1741 cttcagatgg tggagataag ggaaatgccc tggccaaaaa gtggtttcca gtagaccgca
     1801 aagctgccaa ggctcaaggg ttagtagtgg gtggctatca ttatgcgctg cctggaaaca
     1861 tgagcaaaga ccgaaccaaa gatgccaaac ttcaagccgc tagagccgtt cgccaggcga
     1921 atggagctaa agttggggac ttaccgctga ccctagacat ggaagaactg ccatgtgggt
     1981 ggactaatga acagttagct cgctggactg atgtttttct aaaagaagta aaacggctaa
     2041 ccgggcgcac gccgatgatt tacatgaatg gaattttcat caaacgcatt gttggcgcaa
     2101 atgttgctga cttcagtgag tatccacttt ggctagccaa gtggggtcca agcctgggaa
     2161 ctaatcctgg agacgcgtcg atctggccag gtaagtgggt tatctggcaa ttcactgcag
     2221 atggaaaagt ttcagcagtt ccgtccaaga caactgattt aaatgttttt aatggaactc
     2281 aggctgactt ccagcaattt attaatcgtt aagcaaacgc ggcgtcgagt gcaacttcca
     2341 ccattgcgcg gaatccagtt tcacgttcgg ccggagttgt ttgctcgtgt gtgcgaatca
     2401 gatcactgac tgtgcagata gtcagagcgc gtctgcggta tttagctgca attgtgtaca
     2461 gaccagaggt ttccatttca actgcgtaga cgcctaggtt ttctaaaaca tctagcgcat
     2521 tactgtcgtc gtaaaagaca tccattgttg caatcccacc aacatgatgt gaaacatttc
     2581 gtgacttagc cgcacgaacg gctgcttcaa gtaggtcgta atttgctgtt ggggcaaaat
     2641 ctaagccgcc agttgcccta cggtttactc cggagtcagt tgatgcagac attgcaatca
     2701 caacatcacg taacttcaat ggtccgacag caccacaggt gccaactcgg atagcagtct
     2761 gcacatcaaa gtctttaaaa agctcagtta catatattga taatgacggc acacccatac
     2821 cagtgccttg tactgaaaca cggttgcctt tgtaagttcc agtgaagcca aacatgtttc
     2881 gcacactgtt gtatttcact gggttctcaa ggaagttttc cgcaatccac tgagcacgaa
     2941 gtgggtctcc gggtaacaaa attgtgtcgg caatgtcgcc cttattggca ccaatgtgtg
     3001 gagtagtcat gacataaatc tacttcactt ggacacatgc aatgactggg gagttatctt
     3061 ttcgcttgct atctgctaag ccagtgcctt aaggattttt gtccagacta gataagaagc
     3121 ggttaacatc gctgcggctg ccagttggta agtgggttca attcgagtca tggtgccgag
     3181 cgcagcccca gcaaatccaa tcgcattaat taagtcgtga cttatccaag tagtccacca
     3241 ggttattgaa tttttgctag ttgggtttgg ttcattaatt cgctttgatt ttgcctcaac
     3301 tagatcatcg acaattcgct tcggaaacca gaaagacacc actggagtga cccagcccaa
     3361 atatatccag atccgcttta agcgcactgc agttggatta gttttaattt cacgatcgta
     3421 agcaaatgct aagagtctgg ttgtagcgat gtaggaccaa attgaaaaaa taaatatcaa
     3481 aatcgataca ttgtcataaa ttgtcgtaat ctcttcaggc gatttacctt cagcaatttg
     3541 atttgcataa tcttgcgcac tgggaatcga ggccaaagcc acaatccact gtgcagctag
     3601 taatgcggtt gcagatgaaa gtgctgattt tgaaattgcg atcgatctat ccatttggtt
     3661 taaggataca agatgccagt ccggcttagt cggtacatta attaagtgaa aaaatcactc
     3721 tggccagttt tggttgttgt tgctttagtt gggttttgtg cgccaacttc ctcagatact
     3781 tcacttaatt ccacgccgca agtagcacaa aatgcgcaga caactacagc tcagccaagt
     3841 caatcttcgc caactcagaa ttcatcggtt gaagatagtg cgcccatacc ttcaactcaa
     3901 gcagctggcg gtacctcaag agttttaatt cagttaactg aactcgctat aaagggtagg
     3961 gcacctaaaa ctggttatga tcgcgattta tt
//
LOCUS       DBNHVN010000346         1799 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1479084, whole genome shotgun sequence.
ACCESSION   DBNHVN010000346 DBNHVN010000000
VERSION     DBNHVN010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1479084"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="AC16Y1_06645"
     CDS             <1..503
                     /locus_tag="AC16Y1_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5832173.1"
                     /translation="TMLERIHRGQLKTVRLKETRSKPDLFIFAPDPVSDLPYFDPKIS
                     DLAIESDTPFIALATSPKSALITSVINPGSAGCIRCYHLHQSDRDNVWPRLVTQLIGK
                     TIPDPTPTNLILRAALHGYEQICSWLEPRSTDENYWQSLTSAGEVTHLEVLPHTDCGC
                     TLLTKD"
     gene            complement(489..>1799)
                     /locus_tag="AC16Y1_06650"
     CDS             complement(489..>1799)
                     /locus_tag="AC16Y1_06650"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR5832174.1"
                     /translation="STETVRDLAGEVEWAKVNIFSPDVYRTKANELRRSVGLGVTAEQ
                     TAKVYEAYLALLDEQNAMDFEDVLLLNTAILENFPEIAAQVRSQYRNFTVDEFQDVSP
                     VQFELLRLWLGNRDDVCVVGDPAQTIYSFTGATADYLKNFKKFFPRTQEFELTNNYRS
                     TKPIVDVANQVLVKDDQNSLRLKTKISSGPKVTLTDYSSDEEEASAVAEKIQALIKSG
                     IEPRDVAILFRINSQSEVFEAALAQRQIPVTLRGAERFFERPEVKNALLNLRAGLHVS
                     SAHNLTQTVKDILTGLGWRSVAPEGGPATRATWESLNTLVELAVDIEAADKKVTLADF
                     VSLLDQRNEMEYAPSANAVTLASIHAAKGLEWRCVFLIGLSEGLLPISHATKAAEFAE
                     ERRLFYVAITRAKENLAMSWSHSRHKDAKGYREPSPLLRDLVLS"
BASE COUNT          503 a          418 c          357 g          521 t
ORIGIN      
        1 aaactatgct cgagcgaata catcggggtc aactaaagac cgtgcgatta aaagaaacaa
       61 gaagtaaacc tgatttattt atttttgcgc ctgatcctgt ttcagacttg ccctactttg
      121 atccaaaaat ttctgaccta gcgattgagt cagatacgcc atttattgca ctggctacaa
      181 gcccaaaaag tgcgctcatc acttcagtca ttaatccagg aagcgcaggt tgtattcggt
      241 gctaccactt acatcaatca gatcgagata atgtgtggcc aagattggta actcaattaa
      301 tcgggaagac catcccagat ccaacgccaa caaaccttat tttgcgcgct gcattgcatg
      361 gttatgaaca aatttgcagt tggttagaac ccagaagtac tgacgaaaat tactggcaaa
      421 gtttgacgag tgccggtgag gtcacccacc tcgaggttct gccccacaca gattgtggct
      481 gtactttact aactaaggac taagtcacga agtagcggag atggttctcg atagcccttc
      541 gcatctttat ggcgagaatg tgaccaagac atagccagat tttctttagc tcttgttatt
      601 gctacataaa aaagtctgcg ctcttcagca aactcagctg ctttagtggc atgagaaata
      661 ggcaataagc cttcacttag tccaattaga aatacgcatc gccattcaag gcctttcgcc
      721 gcatgtattg atgccagcgt taccgcgttt gcactcgggg cgtattccat ttcatttcgc
      781 tgatctagta gtgacacaaa atcggccaac gtaacctttt tatctgctgc ttcgatatcg
      841 acggcaagtt cgaccaaagt atttagcgac tcccaagtgg ctctagttgc tggacctcct
      901 tcaggagcaa ctgatcgcca acccaaacca gtcaatatat ctttaacagt ttgagttaaa
      961 ttatgtgcgc tactcacgtg taaaccggct cgcaaattta acagtgcatt ctttacttct
     1021 ggtcgttcaa aaaatctctc tgcaccacga agtgttaccg gaatttgccg ctgagctaaa
     1081 gctgcttcaa atacttcaga ctggctattt atccgaaata agattgcaac atctcggggt
     1141 tcaatgccag atttaattaa tgcttggatt ttttcagcta cggcgctagc ttcttcctca
     1201 tcacttgaat aatcggtgag tgtaactttt ggcccactag aaatttttgt cttcaacctc
     1261 agtgaattct ggtcatcttt gactagaact tgattcgcta catccacaat cggcttagtg
     1321 cttcggtagt tattggtcag ttcaaattct tgagttctag gaaaaaactt cttgaaattc
     1381 tttaaataat ctgcagttgc acccgtaaaa gagtagatag tttgagctgg atcgccaaca
     1441 acacatacat cgtcacggtt accgagccaa agtcgaagta gctcaaactg aactgggctc
     1501 acatcttgaa actcatctac ggtgaagttg cgatactgtg aacgcacttg agcagcaatc
     1561 tctggaaaat tctctaggat ggcggtattt agtagtaaaa catcttcaaa atccattgca
     1621 ttttgttcat ctagcaacgc caggtacgct tcataaactt ttgcagtttg ctcagcggta
     1681 acaccaagac caacgctgcg ccttaattca tttgcttttg ttcgataaac atcaggtgaa
     1741 aaaatattta ctttggccca ttcaacttcg ccagccagat ctcgaacagt ttccgtgct
//
LOCUS       DBNHVN010000347         2474 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1479105, whole genome shotgun sequence.
ACCESSION   DBNHVN010000347 DBNHVN010000000
VERSION     DBNHVN010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1479105"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..825
                     /locus_tag="AC16Y1_06655"
     CDS             208..825
                     /locus_tag="AC16Y1_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR5832175.1"
                     /translation="MSDENSDLNAQSAANNSEVERQFAQTKAGEQGLLFDEISMQTMP
                     ATGYRGPVAAGVVGITYRQLDYWARTELVSPTVRPAGGSGTSRLYGFRDILELKIVKR
                     LLDTGVSLQQIRTAVEHLRMRGTTDLSQVTLMSDGASVYECTSNDEVIDLLAGGQGVF
                     GIAIGRVWQEVEGSLAQLPGEKALEELDPLITDELAARRKNRTAG"
     regulatory      868..956
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            1029..>2474
                     /locus_tag="AC16Y1_06660"
     CDS             1029..>2474
                     /locus_tag="AC16Y1_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014691375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HIR5832176.1"
                     /translation="MSVKTANHDSFVPRHIGPNQTELEHILQVLGYGSLDALSSAAVP
                     ANIALPVGLDLFPGASEPEALDELRSLAQANEVLTSMIGLGYYGTHVPGVILRNITEN
                     PAWYTAYTPYQPEISQGRLEALINFQTMVTDFTGLDIASASLLDESTAAAEAMTLMRR
                     ISKSESNIFVVDSDTHPQTLAVLNTRATPLGIELSILDIDANQELPDSFGALFSYPGS
                     SGKIRNMEGLISAAHNQGILVGVVTDLLALTLLKSPGEMGADIAVGSSQRFGVPMAFG
                     GPHAGFMAVKSGLERSLPGRLVGVSVDATGKPAYRLALQTREQHIRRDKATSNICTAQ
                     VLLAVMASMYAVSHGPVGLKEIARRTHSYARTMAAGLIRAGLEIEGDFFDTVRVKVPG
                     KAQTVLNSAYDRGISFWEVDADTVQISVDETTTPQVIEQVWQAFGAAIPGDYPLSFDD
                     VATGENIPTGLQRTSSYLTHPVFNSHHSETEM"
BASE COUNT          668 a          543 c          607 g          656 t
ORIGIN      
        1 tgggtttgaa gtggctaaat ctgctgttgt cgtgcctgca aatacggggt taattctgtc
       61 gaaactctaa actaaaccct ctagtagatg tttagagatg tctcggcgtg tcagatgaca
      121 atgtcgttga ctcgatatat ggctccgcat atagttcttg ttacaggttt ccccgtgtca
      181 aaaggcaccc tgaacacagg agttcgcatg tcggacgaaa actcagatct aaacgcacaa
      241 agtgcggcta ataactctga ggtagagcgt caatttgcac aaacaaaagc tggcgaacaa
      301 ggtctacttt ttgatgagat ctcaatgcaa acaatgccag ccacgggcta tcgcggtccg
      361 gtagccgcag gtgtagttgg cataacttac cgacagcttg attactgggc tcggactgaa
      421 ttggtttcac cgacagttcg ccctgcaggt ggatctggta catcacggct ttatggtttt
      481 cgcgacattt tggaattgaa aattgttaag cggcttctcg atactggtgt atcacttcag
      541 caaatccgaa cagcggttga acaccttcgg atgcgtggta ctaccgattt aagtcaagtg
      601 acattaatga gcgatggggc cagtgtttac gaatgtactt ccaatgacga agtgatcgat
      661 ttactcgccg gcggccaggg tgttttcggg attgcaatcg ggcgagtttg gcaagaggtt
      721 gaaggttcac ttgcccagtt accgggtgaa aaggctttgg aagaattgga tccattaatt
      781 actgacgaat tagctgctcg acgcaaaaat agaactgctg gataactttc gcataaatat
      841 tgatttcgta ttagtctgat atcagcaaga tttaacgtgg gagagcgtgc ttaacagcat
      901 cgccgaaggg gcaaaactcc tcagaacctc tcaggcaaaa ggaccgcgtt gaattagtaa
      961 ctctgaagtg acctgcacaa cagatgggga gagtccgaaa aggacttaaa tctgtttatg
     1021 aggttgtcat gtcagttaaa actgctaatc atgattcgtt cgttccacgt cacattggac
     1081 ccaatcagac tgaactagaa cacatattac aagttcttgg ttatggatcc ttagatgctc
     1141 tttctagcgc tgcagtgccg gcaaatatcg cgttacccgt agggttagat ctttttcctg
     1201 gtgcaagtga accagaagcg cttgatgaac ttcgctctct agcgcaagca aatgaagttc
     1261 taacttcaat gattggtttg ggttactacg gcacacatgt gcctggcgtt attctgcgca
     1321 acatcaccga gaatccagct tggtataccg cctacacacc gtaccaacca gaaatttccc
     1381 aaggtcgtct tgaagcattg attaattttc aaacaatggt cacagatttc actggcctcg
     1441 acattgcaag cgcttcgtta cttgatgaat caacggctgc ggccgaagcg atgaccttaa
     1501 tgcgtcgaat aagcaaaagt gaatcgaata tttttgtagt cgacagtgac acacatccac
     1561 aaactttggc ggtattgaat actcgggcaa cgccacttgg gattgagtta agtattcttg
     1621 acattgacgc taaccaagag ttgccagact cttttggtgc attatttagc tacccaggta
     1681 gtagtggcaa aattcgaaac atggaaggtc tgatcagtgc cgctcataac caaggaattt
     1741 tagttggggt ggtaacagat ttattagcgt taactcttct taagtcacct ggtgaaatgg
     1801 gtgccgacat tgcagtcggt agttcgcaac gtttcggtgt accgatggca tttggcggac
     1861 cgcatgccgg attcatggca gtcaagagtg gtttagaacg atcattacca ggacgcttgg
     1921 ttggcgttag tgtcgatgcg accggaaagc ctgcttatcg tttggcatta caaactcgcg
     1981 aacaacacat tcgtcgcgat aaagcaacta gcaatatttg taccgcacaa gttttacttg
     2041 ctgttatggc gtctatgtat gcggtctccc atggtccagt tgggctgaag gaaattgcgc
     2101 gacgtacaca tagttacgcc agaactatgg ccgctggttt aatccgggca ggtttagaga
     2161 ttgagggcga cttctttgac accgtcagag ttaaagtccc gggcaaggcg caaactgtac
     2221 tgaacagcgc ctacgaccgt ggaatttctt tctgggaggt cgacgctgac acagttcaga
     2281 tttcagtgga tgaaacaact acacctcaag ttattgaaca ggtgtggcaa gcatttggcg
     2341 cagcaatacc aggggactac ccactttcat tcgatgacgt agcaactggt gaaaacatcc
     2401 caactggctt acagcgcaca agtagctatc taacacatcc agtattcaat tcacaccata
     2461 gcgaaacaga aatg
//
LOCUS       DBNHVN010000348         2275 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_735410, whole genome shotgun sequence.
ACCESSION   DBNHVN010000348 DBNHVN010000000
VERSION     DBNHVN010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_735410"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="AC16Y1_06665"
     CDS             complement(<1..543)
                     /locus_tag="AC16Y1_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008616126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5832177.1"
                     /translation="MYILGISAFYHDSAACLLSDGKIIAAAQEERFTRVKHDSRFPTN
                     AIKYCLAEAGISPDQVDQVVFYEKPLPKFERLIETYLAFAPKGFKSFRVSMPIWLTDK
                     IFQKRNLIKQLKSDLAQDVDWESKLLFSEHHLSHAASAFYPSPFESAAVLTMDGVGEW
                     PTTSLALGSGSKLDVIKEIHF"
     gene            complement(547..696)
                     /locus_tag="AC16Y1_06670"
     CDS             complement(547..696)
                     /locus_tag="AC16Y1_06670"
                     /inference="COORDINATES: protein motif:HMM:NF039953.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HIR5832178.1"
                     /translation="MGFLKELVKFLRSRNKMWMLPIIVVMLLLALLLLAAQTSVFATF
                     IYTLF"
     gene            complement(714..1103)
                     /locus_tag="AC16Y1_06675"
     CDS             complement(714..1103)
                     /locus_tag="AC16Y1_06675"
                     /inference="COORDINATES: protein motif:HMM:NF039992.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HIR5832179.1"
                     /translation="MPTLQDRKFGQQMSIALLLVGILGFFRDWNTTVTTTLFVVSAAL
                     LILAYVAPIALSPLNKIWKGVGIVVGKIFQPIEVTLIFGVIFVPVALAMRLFGRDALY
                     LRNRKGDSFWIPRKVQEISVDSFRNQF"
     gene            complement(1181..1939)
                     /locus_tag="AC16Y1_06680"
     CDS             complement(1181..1939)
                     /locus_tag="AC16Y1_06680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013288784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5832180.1"
                     /translation="MTTPGYAVITGASSGIGAASARALAKEGYQVIAAARRVDMLKDL
                     AKEDARIEPFDLDTTDQESIDRLVAHLADKNVTVLFVNAGGAFDGTSIETADVESWIK
                     TYDVNVIGAVRAIKALIPALRKSGRGTIVITGSTAGRIVYENGGSYTAAKHGVAALAE
                     TLRLELSGDPIRVIELAPGMVKTDEFAVKRFSGDTAKAAKIYEGVAKPLTADDVAEAV
                     RWCVTLPDHFNVDLMVIRPLAQAAQHKVHRVFDK"
     gene            complement(1988..>2275)
                     /locus_tag="AC16Y1_06685"
     CDS             complement(1988..>2275)
                     /locus_tag="AC16Y1_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase"
                     /protein_id="HIR5832181.1"
                     /translation="LLALYENDLWLKSATHANSMAMKLYEGVKDVVRVEKPQANALFP
                     YLDPKVTEELQKEFKFYVWDHLTGQVRWMCSWDTSEQDVEAFVSLVRAKVG"
BASE COUNT          660 a          539 c          451 g          625 t
ORIGIN      
        1 gaaatgaatt tccttaatca catctaattt actccctgaa cccaaagcaa gtgaggtggt
       61 aggccactcg cctactccat ccatggtcag aactgcagct gattcaaaag gtgatggata
      121 aaacgcactt gccgcatgtg acaaatggtg ctcactaaac agcaacttgc tttcccagtc
      181 aacatcttgc gcaagatcgg attttagttg cttaattaaa ttacgtttct gaaaaatctt
      241 gtccgttaac caaattggca ttgaaactct aaatgactta aaaccctttg gcgcaaaggc
      301 taagtaggtt tcaatcaatc gttcaaattt tggaaggggc ttttcgtaaa aaacaacttg
      361 gtcaacctga tcaggtgaaa ttcctgcctc agctaagcag tacttgattg cattagtcgg
      421 aaaacgtgaa tcatgcttta ctcgcgtaaa ccgttcttct tgcgccgcag cgataatctt
      481 tccatctgaa agaagacaag ctgccgaatc gtgataaaaa gcggaaatac caagtatgta
      541 catgatttag aaaagcgtgt aaataaatgt ggcaaagacg ctggtctgag cggcgagtaa
      601 tagcagggca agcagtaaca tcaccacaat tattggcagc atccacattt tgttgcgaga
      661 ccgcaggaac ttaactaact cttttaaaaa tcccacttgt cagacccttc ttcctaaaac
      721 tgatttctaa atgaatcgac tgaaatttct tggactttcc gagggatcca aaatgagtca
      781 cctttgcgat ttctaaggta cagcgcatcc ctaccaaata atcgcattgc caatgctact
      841 gggacgaaaa tcaccccaaa aatcaatgtg acttcgatcg gctggaatat tttgccaact
      901 actattccaa cgcctttcca aattttgttt aatggtgaaa gcgcaattgg ggcgacatag
      961 gccaaaatca ggagggctgc gctaaccaca aaaagcgtgg tagttacggt tgtgttccaa
     1021 tcgcggaaga aacctaaaat gcctacaaga agtaaggcga tcgacatttg ttggccaaat
     1081 ttacgatctt gcaacgtcgg cattgaactc agtcgactca cctcttcccc agtaagtata
     1141 actgagcaaa atcaccgact cgtgaaataa gggttttcgg ctatttatca aatactcgat
     1201 gaactttatg ttgcgcggct tgcgctaacg gccgaattac catcaaatca acattgaagt
     1261 gatcaggtaa agtgacgcac cagcgcactg cttctgcgac atcatcggca gttagcggtt
     1321 tggctacacc ttcatatatt tttgctgctt ttgccgtatc gccactaaaa cgttttaccg
     1381 caaattcatc tgtcttaacc atgccgggtg ctaattcgat aactcgaatt ggatctcctg
     1441 acaattccaa tcgcaaagtt tcagcaagtg cagccacccc atgtttggct gcggtataac
     1501 ttccgccatt ttcataaaca attcggcctg cggttgagcc agtaataact atggttcctc
     1561 gacctgattt tcgcagagct gggatcaatg ctttgatcgc tctaacagcg ccaatgacat
     1621 ttacgtcata agttttaatc cacgactcga catctgctgt ttcgatggaa gttccatcga
     1681 atgctccacc agcattgaca aataaaactg ttacattttt gtcagcaaga tgcgcaacta
     1741 gtcgatcgat actttcctga tcggtagtat ccaagtcaaa cggctcaatc cgagcatctt
     1801 cctttgccaa gtctttcaac atatcgactc gacgggcagc ggcaattact tggtagccct
     1861 ctttagctaa cgctcgagca gaggctgcgc ctattccgct acttgctccg gtaataactg
     1921 catatccagg tgtggtcata aggatattta atacccaagc cacaacggcc tgccagcaga
     1981 cctgtaatta acctactttt gctctaacta aagagacgaa agcttcaaca tcttgttcag
     2041 aggtatccca cgagcacatc caacgaactt ggccagtcaa gtgatcccaa acatagaatt
     2101 taaattcttt ttgaagttct tcggtcactt ttggatcaag atatgggaaa agtgcatttg
     2161 cctggggttt ttcaactcgc acgacatctt taacaccctc gtataacttc atcgccattg
     2221 aattggcatg tgttgcgctt ttaagccaga gatcattttc ataaagagca agtaa
//
LOCUS       DBNHVN010000349         2147 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1479525, whole genome shotgun sequence.
ACCESSION   DBNHVN010000349 DBNHVN010000000
VERSION     DBNHVN010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1479525"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2147)
                     /gene="carB"
                     /locus_tag="AC16Y1_06690"
     CDS             complement(<1..>2147)
                     /gene="carB"
                     /locus_tag="AC16Y1_06690"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018539840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR5832182.1"
                     /translation="IAGLGLQASPTTEVLLEESIIGWKEYELELMRDKNDNVVVVCSI
                     ENVDPMGVHTGDSITIAPSMTLTDREYQRLRDLGIAIIRAVGVDTGGCNIQFAVNPDN
                     GRIIVIEMNPRVSRSSALASKATGFPIAKIAARLALGYTLDEIPNDITQETPASFEPT
                     LDYVVTKIPRFTFEKFPNADQTLTTTMKSVGEAMAIGRNFTESLQKALRSIERKEAVF
                     DWSLDPKSIDVADLLTKMKVPHDGRLNKVQLALWAGASIDEVFAATKIDPWFLDQLVL
                     LCEVAREIKAAPGLDREILGFAKRHGFSDAQIGKIRAVSESEVRSIRHTLDVRPVYKT
                     VDTCAAEFAAKTPYHYSSYDLETEVSPRTNPAVIILGSGPNRIGQGIEFDYSCVHASL
                     TLREKGYETIMINCNPETVSTDYDTSSRLYFEPLTLEDVLEVIHAESQAGPILGVITQ
                     LGGQTPLGLAQGLKDAGVTILGTTPEAIHLAEERGAFGEVLAQANLPAPAHGMATSFE
                     DAKAIANRVGYPVLVRPSYVLGGRGMEIVYDETMLADYLTRAAQVNPEHPVLVDRFLD
                     DAIEIDVDALYDGKDLYLGGVMEHIEEAGIHSGDSACALPPYTLNQAQIEEIRGATLA
                     IAKGTGVRGLLNVQYAWAHDGLYVLEANPRASRTVPFVSKATGVQLAKAAARVAVGDS
                     IAKLRTEGFLREVGDGGTMPAGSAIAVKEVVLP"
BASE COUNT          568 a          505 c          480 g          594 t
ORIGIN      
        1 cggtaaaaca acttctttaa ctgcgatggc tgagccagct ggcatggttc cgccgtcacc
       61 gacttcgcgt aagaaacctt cggtccgaag tttggcaatc gaatcaccga cagctactcg
      121 cgcagcggct ttagctagtt gtacgccagt tgcttttgac acgaatggca cagtgcgcga
      181 tgctcgtgga tttgcctcga gtacatagag gccatcatgt gcccatgcgt actgcacatt
      241 taataagcca cgtacgccag tgcctttagc gatggcaagg gttgctcctc ttatctcttc
      301 aatttgtgct tggttgagtg tgtatggcgg tagtgcgcag gcagagtcac ccgaatgaat
      361 accagcttct tcgatgtgtt ccataacgcc acctaagtaa aggtcttttc cgtcataaag
      421 tgcatcaacg tcaatttcaa ttgcatcatc tagaaaccga tcaaccaata ccggatgttc
      481 tggatttact tgggcagctc gagttaagta atcggccaac atagtttcgt cataaacaat
      541 ttccatcccg cggccaccaa gtacatatga aggacgaact aggaccggat agccaacgcg
      601 attggcaatt gctttggcat cttcaaatga agttgccatg ccatgagctg gggcgggcag
      661 atttgcctgt gctaatactt caccaaaagc accacgttct tcggccaagt gaattgcctc
      721 aggagtagta cccaggatgg taaccccggc atctttcaaa ccttgggcta aaccaagtgg
      781 tgtctggcca ccaagttggg tgatcacacc aagtattggt ccagcctgac tttcagcatg
      841 gataacttcg agcacatctt caagcgttaa tggttcaaag tacaaccgac ttgaggtgtc
      901 atagtcagtt gaaactgttt ctggattgca gttaatcatg atggtttcat aacccttttc
      961 tcgaagagtt aatgaggcat gcacacatga ataatcaaat tcaatacctt ggccgattcg
     1021 atttggacct gaccccaaaa taattaccgc tggattagtt cgtggactta cttccgtttc
     1081 taagtcgtaa gacgaatagt gatacggagt tttagctgca aactcggccg cacaagtatc
     1141 gactgttttg taaaccggtc gcacatctaa ggtgtggcga atcgagcgaa cttctgattc
     1201 actgactgcg cgaattttgc caatttgcgc atcagaaaaa ccatggcgtt tagcaaagcc
     1261 aagaatttct cggtctaaac caggtgccgc tttaatttca cgggcaactt cgcaaagtaa
     1321 aacgagctga tctaaaaacc atggatcgat tttggtagca gcaaaaactt catcaattga
     1381 agcacctgcc cagagtgcaa gttgcacttt atttaatcgg ccatcatgtg gcactttcat
     1441 tttggttaac aggtcagcaa catcaattga ttttggatca agagaccaat cgaagactgc
     1501 ttctttacgc tcaatcgagc gaagtgcttt ttgcagactt tcggtgaaat tacggccaat
     1561 tgccatcgct tcgcctaccg acttcatcgt cgtagtaaga gtttgatctg catttgggaa
     1621 tttttcaaaa gtgaaacgtg gaatttttgt aactacgtaa tccaaagtcg gttcaaatga
     1681 ggccggggtt tcttgggtaa tgtcatttgg aatttcatcc aaggtataac caagtgctag
     1741 ccgcgctgca attttcgcaa ttggaaaacc ggtagcttta cttgctaatg cagatgagcg
     1801 agaaacgcgt ggattcattt cgatcacaat gattcgaccg ttatctgggt ttaccgcaaa
     1861 ttgaatattg cagccaccag tatcaacacc cacggctctg ataatcgcga tacctaagtc
     1921 acgcaatctt tgatactcgc ggtccgtcaa agtcatcgaa ggcgcaattg tgatcgagtc
     1981 gccagtatgt acgcccatcg gatctacgtt ttcaattgag caaactacaa ctacgttgtc
     2041 gtttttatcg cgcattaact caagttcgta ttctttccag ccgataatcg actcttccag
     2101 aagtacttca gtcgttggtg atgcttgtaa tccaagtccg gcaattc
//
LOCUS       DBNHVN010000350         1318 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1628408, whole genome shotgun sequence.
ACCESSION   DBNHVN010000350 DBNHVN010000000
VERSION     DBNHVN010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1628408"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1257
                     /locus_tag="AC16Y1_06695"
     CDS             <1..1257
                     /locus_tag="AC16Y1_06695"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HIR5832183.1"
                     /translation="TTNGNSVEQTLAIASFVQLSEGTACGLASGQIRSKADVDLLLEK
                     SKASAIAAGVAEDANEIISGSANPNFNTGPESVDIASLAKVATDLGEVMANKEQEYFG
                     YSEISTDTVYLATSSGLRNRFSQNSSRFELCAKSLDRTRSAWSGQSGSDFSGLSVTDH
                     AASVNQGLRYQKNKIEIEPGKHTVTLSPSAVADLMIYLMWTASAREAAEGRSVFSGPV
                     GGTRIGEQLTERKFTLRSDPHHPGLATIDQVISMGNSTFGSTFDTGAKIDPVNLIDQG
                     KLAALGSSRFTAQLAKLPFTPLADNIIVTDTDGIGDLNNLASRMKDGLLITCLWYIRE
                     VDPQSLLLTGLTRDGVYVVKNGEIVGAANNFRFNESPVGMLKRIIDAGESTYCLPREW
                     ADWFSRAQICPLTIADFNLSTKSEAV"
BASE COUNT          383 a          287 c          295 g          353 t
ORIGIN      
        1 accacaaatg gaaatagtgt tgagcagact ttggcgattg catcttttgt tcaactcagt
       61 gaaggcacag cctgtggcct agcaagtggg caaatacgaa gtaaggctga tgtcgattta
      121 cttctagaaa aaagtaaagc tagcgcgatt gccgcaggcg tggctgaaga tgcaaatgaa
      181 attatcagtg gatcagctaa cccaaatttc aataccggtc ctgaatcagt tgacatagcg
      241 tcactagcca aagtcgcaac agatcttggt gaagttatgg caaataaaga gcaagaatat
      301 tttggctata gcgaaatatc gaccgacaca gtttatctag ccacttcctc tggactacga
      361 aatcgatttt cgcaaaacag cagccgtttt gaactttgtg caaagtcact tgatcgaaca
      421 cgttctgctt ggtctggtca aagtggctct gatttctctg ggctatcagt tacagaccat
      481 gccgcttcag taaaccaggg tctgcgatat cagaaaaata aaattgaaat tgaacccgga
      541 aaacacacgg taacgctttc ccccagcgct gttgccgatc taatgatcta tctaatgtgg
      601 actgcatcgg cacgtgaagc tgctgaaggt agatcggtgt tttctggtcc agttggtgga
      661 acacgaatcg gtgaacaact taccgaacga aaatttactt tgcgttctga tcctcatcat
      721 cctggcttag ccacgattga tcaggtaata agtatgggga attctacttt tggcagcacc
      781 tttgataccg gggcaaaaat tgatccagtg aatctaattg accaaggtaa attagctgct
      841 ttaggtagta gtaggtttac tgcacagtta gcaaaacttc catttactcc gctggctgac
      901 aacatcatcg tcaccgacac agatggcatc ggtgatttaa ataatctggc gagtcgaatg
      961 aaagacggct tacttattac ctgtctttgg tacatccgag aagttgatcc acaatcacta
     1021 ctactaactg gtttaactcg cgacggagtt tatgtcgtaa agaatggaga aattgttggc
     1081 gcggcaaata actttagatt taacgagtca ccagtaggaa tgctgaagcg aataatagat
     1141 gccggcgagt caacttattg tttaccgcgc gaatgggccg actggttttc ccgagcgcaa
     1201 atctgtcccc ttacgattgc cgatttcaac ctatcgacta aatccgaagc agtttaggca
     1261 tcttctaaac ggaaaccaat tttcattccg acctgaacgt gtgcaatttc accatcac
//
LOCUS       DBNHVN010000351         1128 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1109347, whole genome shotgun sequence.
ACCESSION   DBNHVN010000351 DBNHVN010000000
VERSION     DBNHVN010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1109347"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="AC16Y1_06700"
     CDS             <1..523
                     /locus_tag="AC16Y1_06700"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017241093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HIR5832184.1"
                     /translation="DEPFAVLLGDDLVDARTPVLPKMFEAQSKFGGTVLALMEVPSDQ
                     IHLYGCAAVVDTDEKDVVKVTQLVEKPAAGKAPSNYAVIGRYVIHPAVFEILKDLKPG
                     SGDEIQLTDALAKLIELPANQGGGVHAVIFRGKRYDTGDKLSYLKSVISIALDRDDLG
                     KDLRTWLTKYLSD"
     gene            527..1114
                     /locus_tag="AC16Y1_06705"
     CDS             527..1114
                     /locus_tag="AC16Y1_06705"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007382660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5832185.1"
                     /translation="MNKSWPVSLRHGDVRLRPLKKSDAKKWRELRSANQDWMQEWEAT
                     PPIPAIEPAPTFRQSAKQLLKDAKDGRAMPFVVEYKGEFVGQLNVADIIYGSLRGAHF
                     GYWIDHRFAGRGIMTTAVALVTEHLLTTCGLHRVEVAVRPENEPSNKLVQRLGFRLEG
                     QRAKFLHINNDWRDHNIYVMFADDLQDSLFTRLKK"
BASE COUNT          332 a          220 c          275 g          301 t
ORIGIN      
        1 cgatgaacca tttgcggtgt tacttggcga tgacttagtt gatgcccgca caccagtttt
       61 accaaagatg tttgaggcgc aaagtaaatt tggcggaact gtgttggcac tgatggaagt
      121 gccaagtgat caaatccatc tttatggctg tgcagcagtc gtagataccg acgaaaaaga
      181 tgtggttaaa gtaacccagt tagttgaaaa acctgcggct ggaaaagcac catccaatta
      241 cgcagtaatc ggtcgttatg taattcatcc agcagtcttt gaaatcttga aagatttaaa
      301 accgggtagt ggtgatgaaa ttcagttaac ggatgcgcta gcaaaattaa ttgagttacc
      361 agctaatcaa ggtggcgggg tgcacgcggt tatttttcgc ggcaaacgtt atgacactgg
      421 tgacaaactt tcctacttaa agtcagtaat cagtattgct ttagatcgag acgatttagg
      481 taaagattta cgcacttggt taactaaata cctaagtgat taattaatga ataaaagttg
      541 gcctgtatca cttcggcacg gcgatgtgcg cttgcgtcca cttaaaaaaa gtgatgcaaa
      601 gaaatggcgc gaactaaggt cagctaatca agactggatg caagagtggg aagctacacc
      661 accaattccg gcgattgaac cggctccaac ttttcgccag agtgcaaaac aattattgaa
      721 agatgcaaaa gacggccggg ccatgccatt tgtcgtcgaa tacaaaggtg agtttgttgg
      781 tcaactaaat gtggctgaca ttatttatgg ttcactgcgc ggtgcgcatt ttggttattg
      841 gattgatcat cggtttgctg gtcgtggaat tatgacaacg gcggttgcat tagtaaccga
      901 acacttactg accacctgtg gcttacatcg agttgaagtt gcagtccggc cagaaaatga
      961 accgagtaat aagttagttc agcgcttagg tttcagactc gaaggtcagc gagctaagtt
     1021 tttgcacatt aacaatgact ggcgcgacca caacatctat gtcatgtttg ccgatgacct
     1081 acaagatagt ttgttcaccc gactcaaaaa gtaagacacg ggcttaga
//
LOCUS       DBNHVN010000352         6236 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1779873, whole genome shotgun sequence.
ACCESSION   DBNHVN010000352 DBNHVN010000000
VERSION     DBNHVN010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 6236)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6236)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6236
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1779873"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="AC16Y1_06710"
     CDS             <1..1122
                     /locus_tag="AC16Y1_06710"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF033740.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HIR5832186.1"
                     /translation="GVFAIVGIALGIWVANTALTYFYGAATTVDARRVTISTSVFITS
                     VVFGSILGTIAASFLQKFIPRGPLRTLNRIGGSLFSVVTLGLMTWLVSSYLLLIPNAS
                     INAGLNDSTIIGKLDELAPDEFQDYLELARDYATESQLPEVAIEVIIGPEIPEPDPAI
                     TGNENIIAALDSVVRIESIAEECNAKLSGSGFVVSDNLIVTNAHVVAGIVNPEIRLGG
                     KGKTVSGKVIYFDPAVDIALIRTTELVAPALVIGDDLRRNEMAVAAGFPLGGPLELSP
                     ARVRAIATSTDPNIYGEGQVTREIYVLRTAVQQGDSGSALIDENGQVVGVIFAASAND
                     STIGFALMKSELNEALAKGSALTETADTGKCLPVKENKS"
     gene            1174..3135
                     /gene="acs"
                     /locus_tag="AC16Y1_06715"
     CDS             1174..3135
                     /gene="acs"
                     /locus_tag="AC16Y1_06715"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018226250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetyl-CoA synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HIR5832187.1"
                     /translation="MSDDALEILATETRIFPPSAQFIAQANATQSLYDQAKSDLLEFW
                     QAQANRLDWQTNWTQTLDWSSAPVAKWFVGGKLNVAVNCVDRHVQAGNGDRVAFYFEG
                     EPGDSEVITYAQLKDRVCQAANGLLELGIQAGDRVAIYMPMIPQAVIAMLACARIGAT
                     HSVVFGGFSAEALRSRIEDATAKLVITADGGYRKGSAFGLKQAVDEAVSQLPFVEKVL
                     VVKRTSQEVNWDARRDIWWHDLVDKQSTNHDPQYFDAEHPLFILYTSGTTGKPKGILH
                     TTGGYLTHVTYTHNVVFDIKPQSDIYWCTADVGWITGHSYVAYGPTANGATQVIYEGT
                     PDTPHKARWWEIIAKYGVTILYTAPTAIRTFMKWGDEFPASHDLSSLRLLGSVGEPIN
                     PEAWIWYRQTIGGDKCPIVDTWWQTETGGLMISPLPGVTDCKPGSAMRPLPGISAEIL
                     DEQGEVLSHNGNAGYLSLTHPWPGMLRGIWGDVKRYQENYWSRFPGKYFAGDGAKWDS
                     DGAIWLLGRVDDVMNVSGHRISTTEVESALVSHPMVAEAAVVGASDEMTGQGIVAFVI
                     LRAGSIDGPDVAKTLRDHVAKEIGAIAKPRQIMIVPELPKTRSGKIMRRLLRDVAENR
                     TLGDVTTLADSSVMNLISYGLNNSANTED"
     gene            3157..4377
                     /gene="nhaA"
                     /locus_tag="AC16Y1_06720"
     CDS             3157..4377
                     /gene="nhaA"
                     /locus_tag="AC16Y1_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017600437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HIR5832188.1"
                     /translation="MEEALQPIDELTPRSQSRIKKLLHNETNSGALLLSAAAVAMVLA
                     NSAISDWYFDLANTKIGPEILNLNLSLQKWAADGLLAVFFFVAGMELKYELVHGSLSN
                     KANAAVPIAAALGGMIAPALIYFSFNSGQPTIDGWGIPMATDIAFALAVLGVAGRRLP
                     IELRAFLLSLAVVDDLGAIIVIAIFYTTSLALVQLLIGTACIVAFALAQRVNVKNNLL
                     YISLALSSWYFIHESGVHATIAGVALGLAMRVTSVTGETQTPLERALDKVHPWSAGLC
                     VPVFAFFAAGISVTGISLSDAITSPVSLGITLGLVIGKPVGVLGFAWLATKLSKAELN
                     SSLFWGDILAVGLLAGVGFTVSLLITQLAFLPGTEVNPVARLSVLIASIIAAILAVFA
                     LQIRKRIHGNKLAN"
     gene            4429..4824
                     /locus_tag="AC16Y1_06725"
     CDS             4429..4824
                     /locus_tag="AC16Y1_06725"
                     /inference="COORDINATES: protein motif:HMM:NF018980.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR5832189.1"
                     /translation="MEQDKNLTGLINSITENVTTLVRGHIELAKAEVIAAIKNALKSS
                     VLFLIAIAMVNLGMIFLFIGLAFWVSQAFGLASSTGFFITAGVLILVAVVFVGIALAK
                     VKSIFESRKTIDSINATSQTLQSLIPGKK"
     gene            4836..5252
                     /locus_tag="AC16Y1_06730"
     CDS             4836..5252
                     /locus_tag="AC16Y1_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5832190.1"
                     /translation="MDQLSNLIKVERINDAVTAALKSVDELNWNRSVRNNKDLIPLAR
                     RISGYASAALEGASMPADPRQDPDDSPMGKLSNSALGLTAEVDFQLPTFTKTPLQTWA
                     RLHSFIDASENRGRPRTNNEVIDPLHIGAPVPYELI"
     gene            5370..5612
                     /locus_tag="AC16Y1_06735"
     CDS             5370..5612
                     /locus_tag="AC16Y1_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5832191.1"
                     /translation="MIARATTRLVLQSKNVDQLKIVMPEYGFYKLGRNSYAKGLIAYQ
                     SGTIDGVSEWVELHSQAVIIGASSIGLVTELYAQAN"
     gene            5689..>6236
                     /locus_tag="AC16Y1_06740"
     CDS             5689..>6236
                     /locus_tag="AC16Y1_06740"
                     /inference="COORDINATES: protein motif:HMM:NF053434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaE-like family protein"
                     /protein_id="HIR5832192.1"
                     /translation="MQDVLLISQDANLVTEIQKICAVTQASLSCLATASEGEIKNSHT
                     VLIDAELDVVIDHSNVVLVTVSEPGTSIWQKAFATSAKYVAFLPDARPWLLENLTPKL
                     QSSGYAVGLIGATGGLGTSLLATLIATNFSSKNKKVVLAEFSNFTGGLDVLCGVEEVK
                     GTRWSHISNQVLAQDLFRSLPTV"
BASE COUNT         1775 a         1215 c         1418 g         1828 t
ORIGIN      
        1 ggagtgtttg caattgttgg gattgcacta ggtatttggg tcgctaatac cgcactcaca
       61 tatttttatg gcgccgccac aaccgttgat gccagacggg taactatttc aacctctgta
      121 tttatcacca gtgttgtttt cggaagtatt ttaggaacaa ttgcggcgag ctttttacaa
      181 aaatttattc cgcgaggacc actgcgcact ctgaatagaa tcggtggttc attattttca
      241 gtcgtaactc ttggcttaat gacttggtta gtaagcagct acttactttt gattccaaat
      301 gccagtatta atgctggttt aaacgactca acaataattg ggaaactaga tgagttagca
      361 ccggatgaat ttcaagacta tttagagtta gctcgagatt acgcaactga atctcagcta
      421 cctgaagtag caatcgaagt aatcattggt ccggaaattc cagaaccaga tccagcaatt
      481 accggtaacg aaaatatcat tgctgcactt gattcagtgg tccgaattga aagtattgcc
      541 gaagaatgta atgccaaact ttctggcagt ggttttgtgg ttagtgacaa tttaattgtg
      601 actaatgctc acgtagttgc tggaattgtg aatccagaaa tcagacttgg tggtaaaggt
      661 aaaactgtaa gtggcaaagt gatttacttt gatccggcag tagacattgc acttatccga
      721 actactgaat tagttgcgcc cgccttagta attggtgacg atttacgcag gaatgaaatg
      781 gcagtcgcag ccggttttcc attgggtggt ccacttgaat taagtccagc tcgagttaga
      841 gcaattgcaa cttcaactga tccaaatatt tatggtgaag gacaagtaac tcgcgaaatt
      901 tatgttttgc gcacagcagt tcaacaaggt gactctggtt cagctttgat tgacgagaat
      961 ggccaagtag taggtgtaat ttttgctgcc tcagcaaatg attcaacaat tggctttgca
     1021 ttaatgaagt cagagttaaa tgaagcatta gcaaaaggtt ctgctttaac tgagacagct
     1081 gataccggaa agtgtttgcc agtaaaagaa aacaagtctt gacgaaaatt ggctttagcg
     1141 aaactatttg tttagttagg taacggtgaa attatgagcg acgatgccct agagattttg
     1201 gctaccgaga cgcgcatttt tccaccgagt gcgcaattta ttgcgcaagc aaatgcaact
     1261 cagtcacttt acgatcaggc caaaagtgac ttgcttgaat tttggcaagc tcaggctaat
     1321 cgcttggatt ggcaaactaa ttggacgcaa actttagatt ggagtagtgc gccagtagca
     1381 aagtggtttg ttggcggcaa acttaatgtt gcggttaatt gtgtggatcg tcacgtacaa
     1441 gcaggtaatg gcgatcgagt tgctttttat tttgaaggcg aaccaggcga cagcgaagta
     1501 ataacttatg cccagctaaa agatcgagtt tgccaagcag caaatggtct actagaactt
     1561 ggtattcagg ctggcgatcg agttgcaatc tacatgccaa tgattccgca agcagtcatt
     1621 gcgatgttgg catgtgcgcg tatcggtgca acacactcgg ttgtattcgg tggtttttct
     1681 gctgaagcgc ttcgaagccg aatcgaagac gctacagcca aacttgtaat aacagctgat
     1741 ggcggttatc gcaaaggttc tgcttttgga ctgaaacaag cagttgatga agctgtttcg
     1801 caacttcctt ttgttgaaaa agtattagta gttaagcgaa ctagtcaaga agtgaactgg
     1861 gatgcaagga gagatatttg gtggcatgac ttagttgata aacaatcaac aaatcatgat
     1921 ccgcagtatt ttgacgctga acatccattg tttattttgt atacctctgg aactaccgga
     1981 aaaccaaaag gtattttgca cacgacaggt ggttacctaa ctcacgttac gtatacccac
     2041 aatgtagttt ttgacatcaa accccaaagt gatatttatt ggtgcaccgc agatgttggc
     2101 tggataactg gtcattctta tgtggcctac ggcccaacag ctaacggcgc aacacaggtg
     2161 atttacgaag gaactcctga cactccgcac aaagcacgtt ggtgggagat cattgcaaaa
     2221 tatggcgtaa caattcttta cactgcgcca actgcaatca gaactttcat gaagtggggc
     2281 gatgaatttc cagctagcca cgacctttct agtttgcgtt tacttggaag tgttggcgaa
     2341 ccaattaatc ctgaggcatg gatttggtat cgccaaacaa tcggtggtga taagtgtcca
     2401 atagtagata cgtggtggca gactgaaact ggtggactca tgatcagccc attaccgggt
     2461 gtaactgact gcaagccagg ttcagcaatg agaccactgc cgggaattag tgcagaaata
     2521 cttgatgaac aaggtgaagt tttaagtcac aacggaaatg cgggatacct gtcactaact
     2581 catccttggc ctggcatgtt acgtggcatt tggggtgacg tcaaacgtta tcaagaaaat
     2641 tattggtcca gatttccggg aaagtatttt gctggcgatg gagccaagtg ggattccgat
     2701 ggtgcaattt ggttactcgg ccgcgtcgat gacgtgatga atgtttctgg ccatcgcatt
     2761 tcaacaactg aagttgagtc agctttagtt tcacatccaa tggttgctga agccgcagta
     2821 gttggtgcaa gcgacgaaat gactggacaa ggaatagtag cgtttgtaat tttgcgggcc
     2881 ggttctattg atggaccaga tgtagcaaaa actttgcgcg atcacgtggc aaaagaaata
     2941 ggggctattg ccaagcctcg acaaatcatg attgtgcctg aactacctaa aactagatca
     3001 ggaaaaatta tgcgccgatt acttcgtgat gttgccgaga atcgaacact tggcgatgta
     3061 acaacgttgg ctgattcctc agtaatgaac ctaattagtt atggattaaa taactctgca
     3121 aatactgaag attaattttc taatagactc aaagtcatgg aagaagcatt acaaccgatt
     3181 gatgagttaa cacctcgatc tcaatcgcgg atcaaaaaac ttctgcacaa tgaaacaaac
     3241 agtggcgcat tacttttaag cgctgcggct gtggcaatgg tgttagctaa ctcagcaatt
     3301 tctgattggt attttgattt agccaacacc aaaattggtc ctgaaattct caatttaaat
     3361 ttaagtctgc aaaagtgggc ggctgacggt ttacttgcag ttttcttttt tgttgccggt
     3421 atggaactca aatacgaatt agttcatggc tctttaagca ataaggcaaa tgcagctgtg
     3481 ccgatcgccg cagcactggg tggcatgatt gcgcctgcgc taatttattt ttctttcaat
     3541 agcggtcaac caaccattga tggttggggt attcccatgg ctaccgatat cgcatttgct
     3601 ttagctgtac ttggtgttgc tggtcgcaga cttccaatcg aacttcgggc atttttatta
     3661 tctcttgcag tggttgatga tctcggcgca attattgtga ttgcaatttt ttatacgact
     3721 tcgttggcgt tggttcaatt attgattggc acagcctgca tagttgcatt cgccttagcc
     3781 caacgtgtaa atgttaaaaa taatttgctt tacatcagtt tggcacttag tagttggtat
     3841 ttcattcacg aaagtggtgt gcacgcaact attgctggtg tggcattggg cttagccatg
     3901 cgagtaactt cagtaactgg cgagacgcaa actccactcg aacgagcact agataaagtt
     3961 catccttggt cagctggact ttgcgttcca gtctttgcat tctttgctgc tggaatttca
     4021 gtaaccggaa tatctttgag tgacgcaatt acttcaccag tctcacttgg aatcacccta
     4081 ggtttagtaa ttggtaaacc agttggtgtt ctgggctttg cttggttggc cacaaagtta
     4141 tctaaagcag aattaaatag ttcattattc tggggtgaca tattggctgt cggtttatta
     4201 gctggagttg gctttaccgt gtcattactt attacccagt tagcttttct gccgggaacc
     4261 gaagtaaatc cagttgcgcg actctcggta ttaattgcga gcataattgc tgcaatcttg
     4321 gcggtgttcg cactacaaat cagaaagcga attcacggga ataagttagc caactagtgc
     4381 aaatgcaagg atttatttaa ctaaaaaagt tgcactacgc tatttgttat ggaacaagat
     4441 aaaaatttaa cgggattgat taattcaatt accgaaaatg taacgactct tgttcgcgga
     4501 catattgaat tagccaaagc agaagtaatt gctgcgatta aaaatgccct taaatcttca
     4561 gttctatttt taatcgcaat tgccatggtc aatctcggaa tgattttttt gtttatcgga
     4621 ttggcatttt gggttagcca ggcctttggt ctagcaagtt caaccggatt ctttattact
     4681 gcaggcgttc ttattttggt ggctgttgta tttgttggga tcgcacttgc caaagtaaaa
     4741 agtatttttg aatctaggaa aacaatcgat tcaattaatg ccacatctca aactttgcaa
     4801 tccttgatcc cagggaaaaa atagattttc ttttaatgga tcaactttca aatttaatta
     4861 aagttgaacg aatcaacgat gcagttactg ctgcgcttaa atctgttgat gaacttaatt
     4921 ggaatagatc tgttcgaaat aacaaagatt taattccgtt agcgcggcga atcagtggtt
     4981 atgccagtgc agcactcgaa ggtgcgagta tgccagcaga tccacgacaa gacccagatg
     5041 attcaccgat gggaaagctg agtaatagcg cattaggttt aactgctgaa gttgattttc
     5101 aactaccaac ttttactaaa acgccattgc aaacttgggc cagacttcat tcgttcatag
     5161 atgcatctga aaatcgtggt cgaccacgaa ccaacaatga agtgatcgat ccacttcata
     5221 ttggagcgcc agtaccttat gaattaattt agaatcggct tacagattta gttgaaatag
     5281 ttatttcttc gaaagcacct gcattattaa ttgcggctat cgcacatgcc gaattcgcca
     5341 cccttgcgcc atttacaaac ggaagtcaat tgatcgctcg ggctacgacc agattagttc
     5401 tgcaaagtaa aaatgtagac caattaaaaa tagtgatgcc cgaatatggt ttttacaagc
     5461 taggacgtaa ttcttacgcc aaaggtttga ttgcctatca aagtggaact atcgatgggg
     5521 tatccgagtg ggttgaatta cacagtcagg cagtaattat tggtgcatcg agtattggcc
     5581 tagtgactga actttatgca caggcaaatt aattacacaa accaaatctg aatagttact
     5641 agggtccatc aatggactaa atcctgatca gaacaggtca ggagcccaat gcaagacgtg
     5701 cttttaattt cgcaagatgc caacttagtt accgaaatcc agaaaatttg tgccgtaacg
     5761 caagctagtc ttagctgctt agcgactgct tctgaaggtg aaattaaaaa ctcacataca
     5821 gtattaatag acgccgagtt agatgtggtg atcgatcact cgaatgttgt tttggtcacc
     5881 gtaagtgagc caggaacttc gatttggcaa aaagcttttg ccactagcgc aaagtatgtt
     5941 gcctttttac cagatgcacg tccgtggcta cttgaaaatt taacaccaaa acttcaatct
     6001 tccggatacg cagtcggact tattggcgca acaggtggac ttggtacgtc attactggca
     6061 accctgatcg cgacaaattt ttcttctaaa aacaaaaaag tagtgcttgc cgaattttct
     6121 aacttcacag gaggcctaga tgtgctgtgc ggagttgaag aagtaaaagg tactcgctgg
     6181 tcacacattt caaatcaagt attggctcag gatctattta gatctcttcc aacggt
//
LOCUS       DBNHVN010000353         1544 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1035339, whole genome shotgun sequence.
ACCESSION   DBNHVN010000353 DBNHVN010000000
VERSION     DBNHVN010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1035339"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="AC16Y1_06745"
     CDS             <1..936
                     /locus_tag="AC16Y1_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5832193.1"
                     /translation="LSFYRSYHGNTTSAITATGDPRRWPNEFAMGHVHFFGPHLYRSS
                     FWSETQEQESERALAHLAEVIKYEGAKTIGAVLIETVVGTAGILEPPPGYLQGVRKLC
                     DDNDILLILDEVMAGFGRTGEWFAFDNYKVKPDLFVFAKGSNSGYVPVGGVVIGDHIA
                     KTFDERVFPGGLTYSGHPLAAASIVATIDIMKEEGIVENAKMIGEKILGPGLRDLQER
                     HKVIGEVRGLGTFWALDLVKDRKTKEMIAPYGGTSPAMADLISACKKNGLLPFTNYNR
                     LHVVPPCNVTETEAKEGIAILDEVFASIDKYYEGA"
     gene            1003..>1544
                     /locus_tag="AC16Y1_06750"
     CDS             1003..>1544
                     /locus_tag="AC16Y1_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003949522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR5832194.1"
                     /translation="MQILVIGQGAREHAIVVSLLKDKDVSVVCAPGNPGIAQIARTVT
                     VNPVNAQDVLSLAKQVDADLVVIGPEAPLVAGVADILRENGIKVFGPSAAAAQLEGSK
                     AFAKEVMQEAGVPTAASSYCVTVEEARIALEKFGAPYVVKDDGLAAGKGVVVTHDFDI
                     ALEHALVCIDSRDGGAVVVE"
BASE COUNT          429 a          301 c          380 g          434 t
ORIGIN      
        1 ttaagttttt accgcagtta ccacggcaac accacatcag cgattacggc aactggtgat
       61 ccacgtcgtt ggccaaatga atttgcaatg ggtcacgtac acttcttcgg cccacattta
      121 tatcgctcta gtttttggtc tgagactcaa gaacaagaaa gcgaacgcgc attagcgcat
      181 cttgctgaag tgattaagta tgaaggcgct aaaactattg gtgcagtttt gattgagacc
      241 gttgttggta ccgcaggtat tttggaacca cctcctggtt atttacaagg tgtgcgcaaa
      301 ctgtgcgatg acaatgacat tctgttaatt cttgacgaag taatggctgg ctttggccgc
      361 actggtgaat ggtttgcatt cgacaattac aaggtaaagc ctgacttgtt tgtttttgca
      421 aagggatcaa acagtggtta tgtgccagtt ggtggcgtag ttatcggcga tcatattgct
      481 aaaacttttg atgaacgagt ttttccaggt ggattaactt actcaggtca tccattagct
      541 gcagcatcaa ttgtggcaac gattgacatt atgaaagaag aaggcatagt cgaaaatgcc
      601 aaaatgattg gtgaaaaaat tcttggtccg ggacttcgtg atttacaaga gcgtcataaa
      661 gtcatcggtg aagttcgtgg acttggtact ttctgggcac ttgatttagt taaagatcgc
      721 aagactaagg aaatgattgc tccttatggt ggaacatctc cggcgatggc tgatttaatt
      781 tccgcttgta aaaagaatgg cttacttcca ttcacaaact acaaccgctt acatgttgtg
      841 ccgccatgca atgtaactga gaccgaagct aaagagggca tagcaatctt ggatgaagta
      901 tttgcatcaa tcgacaagta ctacgaaggc gcttaatcgc ttattttctt aagttaattc
      961 actaattatt gctttatagc gattcggaat aaacttagga aagtgcaaat cctcgttatc
     1021 ggtcagggtg cgagagaaca cgcaatcgta gttagtctgc ttaaagacaa agatgtcagc
     1081 gttgtttgcg ctccaggcaa tcctggcatt gctcaaattg ccagaactgt gacggtaaat
     1141 ccagttaatg cccaagacgt gctgtcgtta gccaagcaag tggacgctga tttagttgta
     1201 attggaccag aagcaccgct agtcgcaggt gttgcagaca tacttcggga aaatggaatt
     1261 aaagtttttg gcccttccgc cgccgccgct caacttgaag gaagtaaagc attcgccaaa
     1321 gaagtaatgc aagaagcagg agttcctacc gcagcttcca gttattgcgt aacagtagaa
     1381 gaggcacgta ttgcactgga aaaatttggt gcgccctacg tagttaagga tgatgggcta
     1441 gcagctggca aaggcgtagt tgtaacccac gattttgata ttgctttgga gcacgcttta
     1501 gtttgcattg atagtagaga cggcggcgct gttgtagttg aaga
//
LOCUS       DBNHVN010000354         1014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_961050, whole genome shotgun sequence.
ACCESSION   DBNHVN010000354 DBNHVN010000000
VERSION     DBNHVN010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_961050"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="AC16Y1_06755"
     CDS             complement(<1..889)
                     /locus_tag="AC16Y1_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019663492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5832195.1"
                     /translation="MATPQHPFELYDIDTLFNEEQLAIRDVARDFVNDRIKPHISKWF
                     EDGTIPGRELAKEMGQLGMLGMHLEGYGCAGTDATTYGLVCAELEAGDSGLRSLVSVQ
                     GSLAMFAIWKWGSDEQKQQWLPGMAAGELLGCFGLTESDAGSNPSEMRTNAKRDGDDW
                     VLNGSKMWITNGNIADLAIVWAHTDEGIQGFIVPTDTPGFKANLVHHKISLRASVTSE
                     LVFDNVRLPKDAVLPGVQSLRGPLTCLSEARFGILFGVVGAARDCLETAIDYTMTREQ
                     FGRPLASFQLSQQKLADMAI"
BASE COUNT          283 a          242 c          209 g          280 t
ORIGIN      
        1 cgatcgccat gtcagctagc ttttgttgac ttaattgaaa acttgctaat gggcggccga
       61 attgctctcg cgtcatggtg tagtcaattg cagtttctag gcaatcgcgg gcagcaccta
      121 cgacaccaaa taaaattcca aaacgtgctt cggaaagaca agtaagtgga ccacgtagtg
      181 attgcacgcc tggtagaaca gcatcttttg gtaatcgcac attatcgaaa actagttctg
      241 aggtgacaga tgcccgaagc gaaattttat ggtgaacaag atttgcttta aatcctggtg
      301 tgtcagtagg aacaatgaat ccttgaattc cttcatcagt atgcgcccaa acaattgcca
      361 agtcagcaat gttgccattg gtgatccaca tttttgatcc attgagtacc cagtcatcac
      421 catcacgttt agcgttggtc cgcatttcgc ttggattcga acctgcatca gattcagtta
      481 aaccaaaaca gccaagtaat tcgccagccg ccataccagg gagccactgc tgtttttgtt
      541 cgtctgatcc ccatttccag attgcaaaca tggcaagtga gccttgaact gaaacaagag
      601 aacgtaatcc agaatcgcca gcttcaagtt cggcacaaac taatccataa gttgtcgcat
      661 cagtaccagc acagccataa ccctcgaggt gcataccgag catgcccagt tgacccatct
      721 ctttagctaa ttcgcgacca ggaattgtgc cgtcttcgaa ccactttgaa atgtgtggct
      781 tgattcgatc gttaacaaag tcgcgagcaa catcacggat tgccaattgt tcctcgttaa
      841 acaatgtatc gatgtcgtat aactcaaacg gatgctgtgg agttgccatt ttcgcttttc
      901 caatcctgac gaaatttagt tactaattac cttgattatt accttacggt aattggatcc
      961 aatttagcac atattggatc caattaattt tagcccacta gcgtggcgct aaaa
//
LOCUS       DBNHVN010000355         1955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1481766, whole genome shotgun sequence.
ACCESSION   DBNHVN010000355 DBNHVN010000000
VERSION     DBNHVN010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1481766"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1306
                     /locus_tag="AC16Y1_06760"
     CDS             17..1306
                     /locus_tag="AC16Y1_06760"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017605218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HIR5832196.1"
                     /translation="MTVLDVATIKADFPILSRTVRDGKKLIYLDSGATSQKPRQVLDA
                     EREFYERHNSAVHRGAHQLAEEATDAYELARKKIAGFVGAKPNEIVFTKNATEAINLV
                     SYSFLNATLKAQQNKPLPEGAERFILRPGDEILVTEMEHHANLVPWQELTDKLGVTLK
                     WIGLTDEGRLDLNTLGELITEKTKIVALTHQSNLLGTVNDIPTVVEQAKKVDALVLLD
                     ACQSVPHMPVDVQTLGVDFIAWSGHKMLGPLGIGCLWGKSELLNSMPPFISGGSMIET
                     VTMPSSTFALAPARFEAGVPMAAQAVGLGAAVDYLTEIGMDNVAKHEHDLTDLALKLV
                     GEISGVRIIGPTDSSNRGSAVSFVVDGIHPHDVGQILDNTGVAVRVGHHCAWPVCRRY
                     SVPATTRATFYLYNDESDVYALRDGILQAQDFFGVKS"
     gene            1303..1746
                     /gene="sufU"
                     /locus_tag="AC16Y1_06765"
     CDS             1303..1746
                     /gene="sufU"
                     /locus_tag="AC16Y1_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018536854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HIR5832197.1"
                     /translation="MKLESMYQDIILDHYKHPHGKGLVDNADAQVHHVNPTCGDEITL
                     QVRAIPGTENFSIHYDNQGCSISQASASILFDQLNGKSYLEGEKAVDHMIAMMQRASE
                     PDEDLLGDGIALVGVAAYPARVKCALLSWMAWKDAVIQAQAKEGK"
     gene            1747..>1955
                     /locus_tag="AC16Y1_06770"
     CDS             1747..>1955
                     /locus_tag="AC16Y1_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018503334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIR5832198.1"
                     /translation="METLSEDDVMELLRDVVDPELGINVVDLGLVYGVTLDEQNSATI
                     DMTLTSAACPLTDVIEDQTRAALDG"
BASE COUNT          566 a          385 c          480 g          524 t
ORIGIN      
        1 gtaactgcat aaattcatga cggtactaga tgtagctacg atcaaggcag attttccaat
       61 tctgtcgcgc actgtgcgtg atggaaaaaa gttaatctac cttgatagtg gtgctacatc
      121 tcaaaaacca cgccaagttt tagacgcaga acgcgagttt tacgagcgac acaattcggc
      181 ggtacaccgt ggcgcacatc agctagccga agaggctacc gatgcttatg aattggcgcg
      241 taaaaaaatt gctggtttcg taggggcaaa accaaatgaa attgttttta ccaaaaatgc
      301 aactgaggca attaatttag tttcctattc attcctgaat gcgacgctta aggcgcaaca
      361 gaataaaccg ttaccagaag gtgcagaacg atttattctg aggccaggtg acgaaattct
      421 ggtcactgag atggaacatc atgccaattt agttccgtgg caagagttaa ccgacaaact
      481 tggggtgaca ctcaagtgga ttggtttaac tgatgagggt cgactagatc tgaatacgct
      541 gggcgaatta atcaccgaga aaacaaagat cgtcgcactt actcatcaat caaatttact
      601 tggcaccgta aacgacattc cgactgtagt tgaacaagcg aagaaagttg acgctttagt
      661 tttattagac gcttgccaaa gtgtgccgca tatgccagtt gacgttcaaa cacttggcgt
      721 tgactttatt gcatggagtg ggcacaaaat gttaggtcca ctcgggatcg gctgcctctg
      781 gggtaaatca gaattactta attcaatgcc accatttatt tctggtggtt cgatgattga
      841 aacagtaacc atgccaagtt caacttttgc tcttgcaccc gcgcgatttg aagctggtgt
      901 accgatggca gcccaagctg ttggtttagg tgccgcagtc gattacctga ccgaaattgg
      961 tatggacaat gtggctaaac atgaacacga cctaactgac ttggcgttaa aacttgtcgg
     1021 tgaaatttcc ggtgttcgga taattggacc aacggattcc agtaaccgtg gtagtgctgt
     1081 ttcatttgtg gtcgatggaa ttcatccaca tgatgttggt caaatcttag ataacactgg
     1141 ggtagcagtt cgggtcggtc atcactgtgc ctggccggta tgtcgaaggt attcagttcc
     1201 agcgacaacg agagccacgt tttatcttta taacgacgag agcgatgttt atgccttgcg
     1261 cgatggaatt ttgcaggcgc aagatttctt tggggttaag tcatgaaact agaaagtatg
     1321 taccaagaca taatcttgga tcactataaa catccgcacg gtaaaggctt agttgataat
     1381 gcagatgccc aagtgcatca cgtgaatcca acctgtggtg acgaaataac gttgcaagtt
     1441 agagccattc caggaactga aaatttttcg atacattacg acaaccaagg ttgttcgatt
     1501 agccaagcat cagcatcaat tctgtttgat cagttgaatg gaaagtcata cttagagggc
     1561 gaaaaagcag ttgatcatat gattgcgatg atgcagcggg caagcgaacc cgatgaagat
     1621 ttactcggtg atggaattgc tcttgttggt gttgcagcat atccagcacg agttaagtgt
     1681 gcactattgt catggatggc ttggaaagat gcagtcattc aggcgcaagc aaaggaaggt
     1741 aagtagatgg aaaccttaag tgaagacgat gtaatggaat tacttcgaga tgtagtagac
     1801 cctgaattgg ggattaacgt agttgatctt ggcttggttt atggtgtgac acttgatgaa
     1861 caaaattcag caactataga catgacactt acttcagctg cttgtccact aactgacgta
     1921 atcgaagatc agacacgtgc ggcactagat ggtca
//
LOCUS       DBNHVN010000356         1014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1630142, whole genome shotgun sequence.
ACCESSION   DBNHVN010000356 DBNHVN010000000
VERSION     DBNHVN010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1630142"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..>1014)
                     /locus_tag="AC16Y1_06775"
     CDS             complement(71..>1014)
                     /locus_tag="AC16Y1_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011976109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyltransferase family protein"
                     /protein_id="HIR5832199.1"
                     /translation="TGERNLCLAGGVALNCVANGKLLRDGSFDNIWIQPAAGDAGGAL
                     GAALAVHYMMNGNSRTVPTNGDGMSGSYLGSHYSQPEIEKRLTAMGAVFETVTDEQVI
                     ERTAKALADGLAVGWHQGRMEFGPRALGGRSILADPRSPEVQKTLNLKVKFRESFRPF
                     APSVLRDDVSEWFDLKTDSPYMLLVADVAKDKQFAMTDDQNKLFGIDKLNVPRSQIPA
                     VTHVDYSARIQTVHQETNPRYYALIKKFKELTGCPVIVNTSFNVRGEPIVESPEDAFR
                     CLMGTDLDFLAVGNAILRKSDQPQHLIEDYKDKYELD"
BASE COUNT          261 a          258 c          211 g          284 t
ORIGIN      
        1 gtcagcacgc tggaattgag tcttttggtt tttgtgacaa cgaaggtcgc tggttcgcag
       61 taattcgcga ctaatccaac tcgtacttat ctttatagtc ttcgatcaaa tgttgcggct
      121 gatctgactt gcgcaaaata gcatttccga ctgctaagaa atctaagtcc gttcccatta
      181 gacacctgaa tgcatcttct ggtgactcga cgatcggttc gccacgaaca ttaaacgaag
      241 tgttcacaat tactggacat ccagttaatt ctttgaattt tttaatcaga gcgtaataac
      301 gtggattagt ttcctggtgc acagtctgaa tacgcgctga ataatcaacg tgtgttaccg
      361 ctgggatctg cgagcggggt acattcaact tatcgatacc gaataactta ttttgatcat
      421 cagtcatcgc aaattgctta tctttggcca catctgcgac caagagcata tatggtgaat
      481 cagtttttaa atcaaaccat tcagaaacat cgtcacgcag cacacttggt gcaaatggcc
      541 ggaaagactc tcggaattta acttttaaat tcaatgtctt ctgaacttca ggcgaccttg
      601 gatcagccaa gatcgaccgg ccgcctaacg ctcgaggacc aaactccatt cgaccctggt
      661 gccagccgac agctaaccca tctgcaagcg cttttgctgt gcgctcgatc acctgttcat
      721 cggttactgt ctcaaataca gcacccattg ctgttaaccg tttttcaatt tcaggttggc
      781 tgtaatgact tcctaagtac gaaccgctca ttccatcccc attagttgga actgtgcggg
      841 aatttccatt catcatgtag tgcacggcaa gggctgcacc aagggcaccg cctgcatcac
      901 ctgctgctgg ttgaatccaa atattgtcaa aagatccatc gcgcaaaagt tttccgttag
      961 ccacacagtt gagcgccaca cctccagcaa gacataggtt gcgctcgcct gtct
//
LOCUS       DBNHVN010000357         2144 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1556409, whole genome shotgun sequence.
ACCESSION   DBNHVN010000357 DBNHVN010000000
VERSION     DBNHVN010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1556409"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="AC16Y1_06780"
     CDS             <1..394
                     /locus_tag="AC16Y1_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR5832200.1"
                     /translation="KDHGFGEAIFLNSEGLLTEGTGSNIFIVKDKEVLTPSSGCGLLQ
                     GVTRDLVIEWAADFYPISQRKISQDELYAADEIFITSTTRDVSPVSRLASLDKSSRIE
                     NDKAFQVGPVTQEIARIFSAKSNQMSNP"
     gene            394..828
                     /locus_tag="AC16Y1_06785"
     CDS             394..828
                     /locus_tag="AC16Y1_06785"
                     /inference="COORDINATES: protein motif:HMM:NF021193.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HIR5832201.1"
                     /translation="MSETKQRNSLGSNIRFFYRDRRAAAKTNRAIDATWKLIVLVVGL
                     TLVGLGLFFLLFPGPGWATLILGLIVLASEYSWANRLLHPVRKFTSGLAIKLMSDEYK
                     QRRMHIGLATSFLIVVVLYAYWNEWGLTLDGVRQVGNWFLNS"
     gene            870..>2144
                     /locus_tag="AC16Y1_06790"
     CDS             870..>2144
                     /locus_tag="AC16Y1_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005057522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HIR5832202.1"
                     /translation="MPKVLVGNQKVETEIAAGATAFDVVGDKARQVVVALVNGKLVDL
                     ATSLIDGDEVLPVLITDPEGLSVLRHSVAHIVAQATQNLFSDVKLGIGPPTKDGFFYD
                     FGTNRTFTPEDLSAIEDETVRIINSGQRFNRRVIDRNSARKELAHEPFKIRLLGDDDR
                     TQVMAEDVMEVGGSELTIYDNIDLRSNEQKWCDLCRGPHVPDTRYIDARAIKIMRSSA
                     AYWLGDQKNESMQRVYGTAWPTKEDFKKYLNFLEEAEKRDHRKLGVELDLFSFPDEIG
                     SGLAVFHPKGGIVRRQMEDYSRIRHELAGYEFVYTPHITKSTLFETSGHLDWYKEGMF
                     PAMHLDTELNEDGTVKRQGQDYYLKPMNCPFHNLIYKSRGRSYRELPLRLFEFGSVYR
                     YEKSGVVHGLTRVRGMTQDDAHIYCTREQMPNE"
BASE COUNT          627 a          415 c          503 g          599 t
ORIGIN      
        1 taaagatcat ggatttggtg aagctatttt tctcaatagc gaaggtttgt tgactgaagg
       61 aactggcagc aacatcttta tcgttaaaga caaagaagta ctcacaccaa gttctggatg
      121 cggactatta caaggggtga ccagagactt agtgattgaa tgggcagcag atttctaccc
      181 aatttcgcaa cgcaaaatat cgcaagatga actttatgct gccgatgaaa tatttattac
      241 ctcaactacg cgagatgtgt ctcctgttag tcggttagcc agtctagata aatcgtctag
      301 aattgaaaat gacaaagcct ttcaagtcgg ccccgttact caggagatag ccagaatttt
      361 ctcggcgaaa agtaatcaga tgagtaatcc gtgatgtccg aaactaagca aagaaattct
      421 cttggcagca acattagatt tttctatcgt gatcgaagag ctgctgcaaa aacgaatcgc
      481 gccattgatg ccacttggaa attgatcgtt ttagtggttg gactaactct cgttggctta
      541 ggtttattct tcttgttgtt tccaggccct ggttgggcaa ccttgattct gggactaatc
      601 gttctggcat ccgagtacag ctgggcaaat cgacttcttc atccagttcg taaattcacg
      661 tccggcctag ccataaagtt gatgagcgac gaatataaac agcgacgtat gcacattggt
      721 ttggctactt catttctaat tgttgtggtg ctctacgcct attggaatga atggggatta
      781 acactcgatg gtgttcgtca agttggaaac tggtttttaa atagttgaga taggctggtt
      841 ttctaacaag ccaaatcaaa ggatcattcg tgccaaaagt tttagttggc aaccaaaagg
      901 ttgaaactga aattgctgct ggtgctacag cattcgatgt tgtaggggac aaagccaggc
      961 aggtggtcgt tgcactagtc aacggaaaat tagttgatct tgctacatcc ttaattgatg
     1021 gtgatgaagt tttaccggta ctcattacgg atccagaagg tctttctgtt cttcgtcata
     1081 gcgtggcgca catagttgct caggcgactc aaaatttatt tagtgatgta aaacttggaa
     1141 taggtccacc gactaaagat ggatttttct atgatttcgg tacgaatcga acattcacgc
     1201 ctgaagattt atcggcaatt gaagatgaaa cagtgcgaat cataaattca ggtcaaaggt
     1261 ttaatcgacg agttatagat cgaaatagcg cacgtaaaga attagctcat gagccattca
     1321 aaattcggtt acttggcgat gatgaccgaa ctcaagttat ggctgaagac gtcatggaag
     1381 tgggcggaag tgaactcaca atctatgaca acattgattt gcgttccaac gaacagaaat
     1441 ggtgtgatct gtgtcgggga ccacatgttc ctgatactcg atacatagat gcacgggcca
     1501 ttaagatcat gcgatcttca gccgcttatt ggcttggaga tcaaaaaaat gaatccatgc
     1561 aacgggtcta cggcacagca tggccaacta aagaagactt caagaaatat ttgaatttct
     1621 tggaagaggc tgaaaaacgt gatcaccgca aactcggcgt tgaattagat ctattcagtt
     1681 ttccggatga aattggaagc ggtcttgcag tattccatcc aaaaggtggc attgttcgac
     1741 gccagatgga agattactca cgaattcgac atgaattagc tgggtatgag tttgtctaca
     1801 cgccacacat tacgaagtcc acgttgttcg aaacatctgg acatcttgat tggtacaaag
     1861 agggcatgtt cccggcaatg caccttgata ctgaactgaa tgaggatgga actgttaaac
     1921 ggcaaggaca ggactattac ttaaagccga tgaactgtcc gtttcacaat ttaatttata
     1981 aatccagggg acgcagttac cgagaacttc ctttgcggtt gtttgaattt ggttctgtat
     2041 accggtatga aaaatccggt gtggtacatg gacttactcg cgtcagaggt atgacacaag
     2101 atgacgcaca catttattgc acccgcgagc agatgccaaa tgaa
//
LOCUS       DBNHVN010000358         2967 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1111366, whole genome shotgun sequence.
ACCESSION   DBNHVN010000358 DBNHVN010000000
VERSION     DBNHVN010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1111366"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="AC16Y1_06795"
     CDS             <1..887
                     /locus_tag="AC16Y1_06795"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HIR5832203.1"
                     /translation="LSNQEMFDVLAEAGAVICAASETLAPADRRIYALRDVTATVESI
                     PLISASIMSKKIAEGTSALVLDVKVGSGAFMKTVEQAKELASTMVAIGNDAGVNTRAL
                     LTAMDVPLGRTAGNALEVQEALDVLAGGGPSDVVELTLLLANEMIDAAGVKTTKDPQT
                     ALSDGSAMDVWRKMISMQGGDVNAPLPKAKETHTVIAQRDGYLTKLDALGVGIAAWRL
                     GAGRARKEDSVQAGAGVTWSATHGETVTMGQELFTLHTDDAARFELALEPLMNSFEIG
                     SETEVKDRFPLVIERITQ"
     gene            910..1263
                     /locus_tag="AC16Y1_06800"
     CDS             910..1263
                     /locus_tag="AC16Y1_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5832204.1"
                     /translation="MPELIEEPTLIPVPGGKSIAEFIGRVNSNDEKISVAVMQAPAGW
                     SEPAQTPEFDEFTIVLSGSIEVEHEGGSTLVKANQAIVTRAGERVRYLTKEAAQYVAI
                     CLPAFDVDLVNREED"
     gene            1263..2351
                     /locus_tag="AC16Y1_06805"
     CDS             1263..2351
                     /locus_tag="AC16Y1_06805"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016907827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HIR5832205.1"
                     /translation="MLTQEVLKRVPKAVLHDHLDGGLRVKTILELAKETGYNKLPFDN
                     EPELTNWFHTAVQGSLEVYLETFAHTVGVMQTPAAITRVARECVEDLANEGVVYAEIR
                     FAPELHTSKGLTYEEVIDAVLEGFRQGSDQSAQAGKPIRVGALLTAMRMADVSLEIAK
                     LVVKYRHQGVVGFDIAGKEKGYPPTEHLEAFQFLQRENAHFTIHAGEAFGLKSIWEAI
                     QFCGTERLGHGVRIIDDITVNPDGSVSIGELAAYVRDRRIPLELCPKSNVDTGAAESI
                     AKHPIGLLRKLNFRVTLNTDNRLMSDTSMTKEMFWLAEAFNYTLKDFEWLTINSLKSS
                     FIPFDERLAIINNVVKPGYAKIKSDENL"
     gene            complement(2371..>2967)
                     /locus_tag="AC16Y1_06810"
     CDS             complement(2371..>2967)
                     /locus_tag="AC16Y1_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007540685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5832206.1"
                     /translation="QFVHEIQISTGKTLKQATTEVERAIDSWVWHAGWPDKIDAVAGN
                     MNPVAGPFFNISTLVPTGVVAAISPSSSSLVGLVNTIAPAIVSGNSIVVLANTDHPLP
                     AATLSEVFETSDLPAGVVNLLTGLESELASWLATHQDVNAIDLEGASKVSDLEIDAAI
                     NLKRVIRPGEFNRNNSIGNFQHICSLMEVKTVWHPKGH"
BASE COUNT          891 a          578 c          682 g          816 t
ORIGIN      
        1 aactttctaa tcaggaaatg tttgatgttt tagcagaagc aggtgcggta atttgtgcag
       61 ctagtgagac tctcgctcca gcagatcgac gtatctacgc attacgtgat gtcactgcca
      121 cagtcgaatc aatccctttg atctctgcat caattatgag taaaaagatt gccgaaggaa
      181 ctagcgcttt agtccttgat gtaaaagttg gctctggtgc gtttatgaaa acagttgaac
      241 aagcaaaaga attagctagc acgatggtgg caatcggaaa cgatgcaggt gttaataccc
      301 gtgcattgtt aactgcaatg gatgttccac ttggacgaac tgcgggcaac gcactagaag
      361 ttcaagaggc tctagatgta ttggctggtg gcgggccgag cgatgtagtt gaactaaccc
      421 ttttattagc aaatgaaatg attgacgccg ctggtgttaa aacaactaaa gatccccaaa
      481 ctgcactgag cgatggaagt gcgatggatg tttggcgcaa gatgatttca atgcaaggtg
      541 gcgacgtgaa tgcgccactt ccaaaagcaa aagaaactca tacggtaatt gcgcaacgtg
      601 atggctactt aacaaagtta gatgcacttg gtgtcggtat tgcagcttgg cggttaggag
      661 ctgggcgggc ccgcaaagaa gattcagttc aggcaggcgc tggtgtaact tggtcggcaa
      721 cccatgggga gacagtaact atgggtcaag aattatttac tctacataca gatgatgcag
      781 cgcgatttga acttgcgcta gaacctttaa tgaatagttt tgaaatcgga agtgaaacag
      841 aagtgaaaga tcgctttcca ttggtgattg agcgaataac tcaataatta aataacgtag
      901 agttagtaca tgcctgaact aattgaagaa ccaacactta ttccagtacc tggtggaaaa
      961 tctatcgctg agtttattgg ccgagttaat agcaacgatg aaaaaatttc agttgcagta
     1021 atgcaagcgc cagctgggtg gagcgagccg gcacagactc cagaatttga tgaatttaca
     1081 attgtgctca gcggatcaat tgaagttgaa catgaaggcg ggtccacctt agttaaagct
     1141 aaccaggcaa tcgttacgcg agcaggcgaa cgggttagat acttaactaa agaagctgca
     1201 caatacgtag caatttgctt acctgcattc gatgttgatc tagttaaccg agaagaagat
     1261 tgatgcttac tcaagaagtt ttgaaacgtg ttcctaaagc ggttttgcat gatcaccttg
     1321 acggtggcct aagagtaaaa actattttgg aactagctaa agagactggt tacaacaaac
     1381 tgccatttga taatgaacct gaattaacaa attggtttca cactgcagtt caagggtcgc
     1441 ttgaagttta tttagaaacg tttgcccaca cggtaggtgt tatgcaaacg cccgcggcaa
     1501 ttactcgagt ggctcgtgaa tgtgttgagg acttagcaaa cgaaggtgtg gtttatgccg
     1561 aaattagatt tgccccggaa ctacatacgt caaaaggatt aacctacgaa gaagtaatcg
     1621 atgcagtgct tgagggattt cggcaaggta gtgatcaaag tgctcaagct ggcaaaccta
     1681 ttcgcgttgg tgcgctatta accgcaatgc ggatggcaga tgtttcactg gaaattgcca
     1741 agttagtagt taagtatcgg catcaaggtg ttgttggttt tgacatagct ggcaaagaaa
     1801 aaggttatcc gccaacagag catcttgagg cttttcaatt tttacaacgt gagaatgcac
     1861 atttcacaat tcatgctggt gaagcatttg gattaaaaag tatttgggaa gcaattcaat
     1921 tctgcggcac cgaacgactt ggtcacggtg ttcgcattat tgatgacata accgtaaatc
     1981 cagatggttc agtatctata ggtgaattag cggcttacgt tcgcgatcgc cgcattccac
     2041 ttgaactttg cccaaaatca aatgttgata ctggcgccgc tgaaagtatc gctaaacatc
     2101 cgattggttt acttcgcaaa ctaaatttcc gcgtcacctt aaataccgat aatcgactta
     2161 tgtctgatac ttcaatgact aaagaaatgt tttggcttgc cgaggcattt aattacactt
     2221 tgaaggattt tgaatggctg acaatcaata gtctgaaaag ttcattcatt ccatttgatg
     2281 aacggcttgc cattatcaat aatgtggtaa aaccgggata tgcaaaaatc aaatcagatg
     2341 aaaatctttg agctcatctg attttaaaat ttagtgtccc tttggatgcc agacagtttt
     2401 aacttccata agggaacaaa tatgttgaaa atttccaatg gaattatttc ggttaaattc
     2461 gccaggtcta atcacgcgtt ttaaattgat ggccgcatca atttccaggt cactgacttt
     2521 cgatgcacct tcaagatcta tggcatttac atcttgatgt gttgctaacc acgaagctaa
     2581 ttcactttct aagccagtca acaaattaac aactccggca ggaaggtcac ttgtttcaaa
     2641 aacttcgctc aatgtagctg ccggaagggg atgatccgta ttcgccaaaa caacaattga
     2701 attacccgaa actattgcag gtgcaatagt gttcaccagc ccaactaaag aggagctcga
     2761 tggagaaatt gcagctacga ctccagtagg tactaatgtc gagatattaa aaaatggtcc
     2821 agctactgga ttcatgttgc cggctactgc atcgattttg tctggccacc ctgcatgcca
     2881 aacccaacta tcaattgcac gctcaacttc agtagttgct tgttttaaag ttttaccagt
     2941 tgaaatttga atttcgtgaa caaattg
//
LOCUS       DBNHVN010000359         2488 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1334577, whole genome shotgun sequence.
ACCESSION   DBNHVN010000359 DBNHVN010000000
VERSION     DBNHVN010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1334577"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2488)
                     /gene="gyrA"
                     /locus_tag="AC16Y1_06815"
     CDS             complement(<1..>2488)
                     /gene="gyrA"
                     /locus_tag="AC16Y1_06815"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009716475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR5832207.1"
                     /translation="YRPDKAFSKSARVVGEVMGQYHPHGDSAIYDTLVRLTQRWTLRY
                     PLVQGQGNFGSPGNDSAAASRYTECRMAPLAMEMVRDIDQETVDFTPNYDGKNLEPTV
                     LPSRIPNLLINGSSGIAVGMATNIPPHNLTEVANGIQWALEHPDATREELLSALTERI
                     TGPDFPTGGLIVGRAGIKDAYRTGRGSITMRAVTEITEDKRGRSIIVITELPYQVNPD
                     NLMERIAELVDTGKISGIADLVDESSSRTGMRIVVVLKKDAVPKVVLNNLFKFTQLQD
                     NFGANMLALVDGVPRTLSLDAFVSHWINHQIEVIARRTAYRLRIAQERVHILIGLLKA
                     IERIDDVIKLIRASESASVAQQGLMKLLKIDELQAQAILDMQLRKLAALERNELQSQH
                     DKLMTEIADYKDILSNVKRQYKIVSEELAELVEKFGDERRSRIVAAEDDVSHEDLIAQ
                     EPIVVTLTQNGYVKRTKAVLYRAQKRGGRGVKGAALKQDDVVTHLFVTTTHHWILFFT
                     NRGRVFRVKAYELVDTGRDARGQHVANLLQLGQDEFVAQVLDLRDYEQAPYLVLATRN
                     GVVKKTKLTEYDSNRSTGLIAINLRDDDSVVSAQLISDDDDLLLVSKFGNSVRFNASD
                     DTLRPMGRSATGVIGIRFKNKEDHLLTMEVAHPDAYVVTITNGGFAKRSAMSEWNAKG
                     RGTLGVRAMRLSEKRGGLAGALVCEADDELFAVASNGVVIRTRVEEIRAAGRDTMGVS
                     FMKVGDDEEVVAVARSSAATLAKLEQLKLEQPANGLVEVEQDIEQPEVEAKPVAKTPA
                     KNETAPAKVKPAVQTKPEVKAKPVDKSKKTA"
BASE COUNT          642 a          558 c          512 g          776 t
ORIGIN      
        1 ctgcagtctt ttttgattta tcgactggtt tggcttttac ctctggcttt gtttgcaccg
       61 caggtttaac ttttgccgga gcggtttcat ttttggctgg agttttagcg actggttttg
      121 cttctacttc tggttgttcg atatcttgtt cgacttctac taaaccgttt gctggttgtt
      181 cgagtttaag ttgttcaagt tttgccaaag ttgctgctga agatcttgct acggcaacga
      241 cttcttcatc gtcgccaact ttcatgaatg aaacacccat agtgtcgcgg ccagcagcac
      301 gaatttcttc gactctggta cgaattacaa caccattgct tgctacagca aataactcat
      361 catctgcttc acaaaccaat gcgccagcta atccaccacg tttttcggaa aggcgcatag
      421 cacggacacc taaagttccg cggccttttg cattccattc actcatcgca cttcgttttg
      481 caaaaccacc attagtgatt gtcacaacat aagcatctgg atgtgcaact tccatagtta
      541 aaaggtggtc ttctttattt ttaaatcgga ttccgattac gcctgttgca cttcggccca
      601 taggtcgcaa ggtgtcatcg ctggcattaa atcgaactga gttaccgaat ttagaaacta
      661 gtaataaatc atcatcatca gaaattaact gtgcagagac tactgaatcg tcatcacgta
      721 agttgatcgc aattagtcca gtgcttcggt ttgagtcgta ttcagttaat ttggttttct
      781 taaccactcc atttcgggta gctaaaacta aatacggagc ttgttcgtaa tcacgtaaat
      841 ctaaaacttg cgcaacaaat tcatcttggc caagttgtaa caagttagct acatgttgtc
      901 cgcgggcatc acggccagta tcaactaatt catatgcttt aacacgaaat actcgcccac
      961 gattagtaaa gaataaaatc caatggtgag ttgtagttac aaataaatga gttactacgt
     1021 catcttgttt aagcgcagcg cctttaacgc cgcggccgcc gcgtttttgt gcacggtata
     1081 gcaccgcttt ggtgcgcttt acataaccgt tttgagttag agtcaccaca attggttctt
     1141 gagcaattaa atcttcgtgg ctgacatcat cttcggctgc cacaattcga gatctgcgtt
     1201 catcaccgaa tttttcaacg agttcggcta gctcttcgct gactatcttg tactggcgtt
     1261 ttacgttact caaaatatct ttgtaatctg caatctcagt catcaacttg tcatgctgtg
     1321 attgcaactc gttgcgttca agagctgcaa gttttcgcaa ctgcatatct aaaattgcct
     1381 gagcttgaag ttcatcaatt ttgagtagct tcatcaaacc ttgctgcgca actgatgctg
     1441 attccgaagc gcgaattaat ttaataacgt catcgattcg ttctattgct ttaagcaacc
     1501 caattaatat gtgaacgcgc tcttgggcaa tccgaagtcg atatgcggta cgtcttgcga
     1561 ttacctcaat ctgatggtta atccagtgtg agacaaatgc atctagtgag agcgttcttg
     1621 gcacaccatc aacaagagct agcatgtttg cgccgaagtt gtcttgtagt tgagtgaact
     1681 taaataagtt atttaaaaca actttgggaa ctgcatcttt tttaagtaca acaacaatgc
     1741 gcatgccggt acgtgaactt gattcatcaa ctaaatcagc gattccagaa atttttccgg
     1801 tgtcaactaa ttcagcaata cgttccatca agttgtctgg gttaacttgg tatggcaatt
     1861 cagtaataac aatgattgag cgaccacgtt tgtcttcggt tatctcagtt actgctcgca
     1921 ttgtgattga accgcggcca gttcgataag catctttaat tcctgcgcgc ccaacaatta
     1981 agccacccgt tgggaaatct ggccctgtaa ttcgttcagt cagcgcagat agtagttctt
     2041 cgcgtgtggc gtcaggatgc tcgagtgccc actgaatacc gtttgctact tcagttaaat
     2101 tatgtggcgg aatatttgtc gccataccaa cggcaatacc tgatgaaccg ttaattaata
     2161 agttaggaat tcgacttggt aaaactgttg gttctaaatt tttgccatcg taatttggcg
     2221 tgaagtcgac agtttcttga tcgatatcac gcaccatttc catagcaagt ggtgccattc
     2281 gacattcggt ataacgagat gctgcagccg agtcgtttcc aggtgagcca aagttacctt
     2341 gaccttgaac taatggataa cgaagtgtcc aacgttgtgt taatcgaacg agagtgtcat
     2401 agatcgctga gtcaccgtgt ggatgatact gacccataac ttcgccgaca acgcgagcac
     2461 ttttactaaa tgctttgtct ggtcgata
//
LOCUS       DBNHVN010000360         2320 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1483411, whole genome shotgun sequence.
ACCESSION   DBNHVN010000360 DBNHVN010000000
VERSION     DBNHVN010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1483411"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="AC16Y1_06820"
     CDS             complement(<1..585)
                     /locus_tag="AC16Y1_06820"
                     /inference="COORDINATES: protein motif:HMM:NF022702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 N-terminal domain-containing protein"
                     /protein_id="HIR5832208.1"
                     /translation="MKQKASKIAAIALASALVLTACGGEDETVAPQDQPVSENTLWPL
                     TQLPGPEDALTQPVLAIKVENDPSIRPQYGLDGADIVVEELVEGGLTRFVAFFQSNVP
                     DEVGPIRSARHVDASIVSPLADYFVMSGASGVTFAYIDQNMPINIMRVLEGGPGMHRT
                     NYHPAPHNVFVYPLDVISGIQTAKSPTGGLFVYPA"
     gene            complement(610..2058)
                     /locus_tag="AC16Y1_06825"
     CDS             complement(610..2058)
                     /locus_tag="AC16Y1_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5832209.1"
                     /translation="MAAKKSAPQPVETKKATVAKKAPAKTVATKKSTVKASASSTEAK
                     KNSVPVKKSAVKESAKKDTAKKVPATKTAQKPAIKDSSGKKAAKAGIEKPVKGRGKAK
                     AVSDDDEEIVDLEEIDLEADIEVDIVVAAEVEAEAETILAEDLADVEAAAAEIADADA
                     LVISDVDDTDEPVQQVVTAGATADPVKDYLKQIGKVPLLNAEEEVELAKRIEAGLFAE
                     ELLATRGPFKDRRQGDYEWIAEDGQRAKNHLLEANLRLVVSLAKRYTGRGMLFLDLIQ
                     EGNLGLIRAVEKFDYTKGYKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLA
                     RVQRQMLQDLGREPTPEELARELDMTPEKVVEVQKYGREPISLHTPLGEEGDSEFGDL
                     IEDSEAIVPADAVSFTLLQEQLDSVLDTLSEWESGVVKMRFGLTDGQPKTLDEIGKVY
                     GVTRERIRQIESKTMSKLRHPSRSQVLRDYLD"
BASE COUNT          560 a          555 c          510 g          695 t
ORIGIN      
        1 agctgggtaa acaaacaggc cacctgttgg tgacttggca gtctgaattc cgctgatcac
       61 atctagtgga taaacgaaaa cgttgtgcgg tgctggatgg taattagtcc ggtgcatacc
      121 tggaccacct tcgagtacgc gcatgatgtt aatcggcata ttctgatcga tgtaagcaaa
      181 agtcacacca gaggcgccag acataacaaa gtagtcagca agtgggctaa caatcgaggc
      241 gtcaacgtga cgtgcacttc gaattgggcc aacttcatct gggacatttg attggaagaa
      301 agcaacgaaa cgtgttaggc caccttcaac aagttcctct acgacaatgt ccgctccatc
      361 tagtccgtat tgaggacgga ttgacggatc gttttcaact ttgatcgcaa gtacaggttg
      421 agttagtgca tcctcgggtc caggcaactg ggtaagtggc caaagtgtat tttcagatac
      481 cggctgatct tgcggagcaa cggtttcgtc ttccccaccg caagcagtga gtactaaagc
      541 tgatgccaat gcaatggccg cgatcttgct tgctttttgt ttcatctttt tccctcgggt
      601 cattgctttc tagtctaagt aatctcgtag tacttgagag cgagatgggt gtcggagttt
      661 cgacattgtt ttgctctcga tttggcggat tcgctcgcgg gtaaccccgt ataccttgcc
      721 aatttcgtcc aaagtctttg gttgaccgtc agtaagaccg aatctcattt tcacaacgcc
      781 ggattcccac tcagaaagcg tatcaagcac agaatcaagc tgttcttgta acagagtgaa
      841 tgaaacagca tcagccggaa caatggcttc agagtcttca atcaaatcac caaattcgct
      901 atcgccttct tcgcctagtg gcgtgtgaag tgagattggt tcacgtccgt atttctgaac
      961 ttcgacaact ttttctggcg tcatgtctag ttcacgagct aactcttctg gagttggttc
     1021 acggcccaaa tcctgaagca tttgtcgctg cacacgtgca agtttgttaa tgacttcaac
     1081 catgtgaact ggaatacgaa tggtgcgagc ttggtcagcc atcgcgcgag taattgcctg
     1141 gcgaatccac caagttgcat aagtcgaaaa cttataaccc ttggtgtagt caaatttttc
     1201 aacagcgcga attaaaccta agttaccttc ttgaattaag tccaagaaca acatgccgcg
     1261 gccggtatag cgcttagcta acgaaacaac aagacgtaag ttcgcttcta gaaggtgatt
     1321 cttagcgcgt tgcccgtcct cggcaatcca ctcataatca ccttgacgac gatctttaaa
     1381 tggtccacga gtagcaagta attcttcagc gaataaacca gcttcaattc tttttgctaa
     1441 ctcaacttct tcttcagcat taagtagcgg aactttaccg atttgcttca gataatcctt
     1501 cactggatct gcggttgcgc cagcagttac tacttgttgt accggttcat cagtgtcatc
     1561 aacatcagag ataaccaaag catctgcgtc tgcaatttca gccgcagcag cctcaacatc
     1621 cgctaaatct tcggccaaaa tagtttcagc ttcggcttca acttctgcgg caactacgat
     1681 atcaacttcg atgtcggctt caagatctat ttcttctaga tcgacaattt cctcatcgtc
     1741 atcgcttacc gcttttgcct tgcctctacc cttgactggt ttttcgatac cagcttttgc
     1801 tgctttctta cctgatgaat cttttattgc tggcttctgg gcagttttag ttgccggaac
     1861 ttttttagct gtatcttttt ttgcagattc ttttactgcg gacttcttaa ctggaactga
     1921 attcttttta gcttcggtgc tagaagcaga agcttttaca gttgatttct ttgttgcaac
     1981 agttttagca ggtgcttttt ttgctaccgt tgcttttttt gtttcaactg gttgtggggc
     2041 agattttttc gcagccacta acgcctctaa tttccttggt cgaccaataa caagggccct
     2101 tgtcaagcaa atgcttaact tgggcggcac ccttatgggt acttgagtat tctaacctga
     2161 ctttaagcca ttgctagacc tgggtcgaat acctggctag ctaccgcatt aatactgact
     2221 tggcccgaag aattggaatc gacttgctgg ttgattgggt tactaagtcg ccgccggtcg
     2281 cgaaagtacc aattccactg actttgtaag ttgcttgcca
//
LOCUS       DBNHVN010000361         3154 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1633474, whole genome shotgun sequence.
ACCESSION   DBNHVN010000361 DBNHVN010000000
VERSION     DBNHVN010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1633474"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2619)
                     /gene="fdhF"
                     /locus_tag="AC16Y1_06830"
     CDS             complement(<1..2619)
                     /gene="fdhF"
                     /locus_tag="AC16Y1_06830"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003891493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HIR5832210.1"
                     /translation="MFTQGIAPIRNGIGEPDFGTPKSNSDQQVTLLIDGAPVTVPAGT
                     SIMRAAGENGCSIPKLCATDSLEPFGSCRMCLVEIDGRKGTPASCTTPAEDGMVVRTQ
                     TAKLSKLRRNVMELYISDHPLECLTCAANGDCELQDMAGVVGLRDVRYGYDGENHLDA
                     PKDTSNPYFDFEGSKCIVCNRCVRACDQIQGTLALTIEGRGFNSKVSPGGLNFLSSEC
                     VSCGACVQACPTGTLEEKSVIQLGMPTRTVQTTCAYCGVGCTFNAELRGDELVRMVPA
                     KTGGANEGHSCVKGRFAFGYATHKDRVLSPMIREKITDDWQEVSWDEAINYTAAKFKE
                     IQAKFGDNAIGGITSSRCTNEEIFVVQKLIRAAFGNNNVDTCARVCHSPTGFGLSSTF
                     GTSAGTQDFKSVGKSDVILLIGANPTDAHPVFASRLKRRLRQGAKLIVVDPRKIDLVE
                     SARVKAQHHLQLQPGTNVAIINALAHVIVTEGLVAKEFINERCDVEAFDRWMNFIALP
                     ENSPEVVQAQSGVPADEIRQAARLFATGGNGAIYYGLGVTEHSQGSTMVMGMANLAMA
                     TGNIGREGVGVNPLRGQNNVQGACDMGSFPHELSGYRHVSDDAVREQFESVWDVKILP
                     EPGMRIPNMLDNAISGDFRGIFVHGEDIAQSDPNTNHVKAALSAMDLVVVQDLFINET
                     ANFAHVFFPGTSFLEKDGTFTNAERRINRVRPVMAPKNGLQEWEIVAKLATAMGYPMH
                     YDNAAQIMDEIAVMTPTFKNVSFELLDRVGSLQWPVNDAAPDGTPIMHLNGFVRGKGL
                     FIETPFVPTEERSNRKFPLLLTTGRILSQYNVGAQTRRTANSTWHQEDLLEIHEADAQ
                     MRGISDGQKVAITSRIG"
     gene            complement(2649..>3154)
                     /locus_tag="AC16Y1_06835"
     CDS             complement(2649..>3154)
                     /locus_tag="AC16Y1_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HIR5832211.1"
                     /translation="IARGGIVETAFGITLGQLVDDFGGGTHSGRPIKTVQVGGPLGAY
                     LDKSAWDVPMAYESLAAAGGMLGHGGVVVFDDTVNMAEQARFAMEFCAEESCGKCTPC
                     RIGSTRGVEVIDKIVAGEDQAQNLELLEDLCEVMTDGSLCAMGGLTPLPVRSALKLYP
                     SDFGIRS"
BASE COUNT          823 a          781 c          685 g          865 t
ORIGIN      
        1 accgatgcgg ctagtgatgg cgactttttg accatcacta attccacgca tttgtgcatc
       61 agcttcatga atttccaaaa ggtcttcctg atgccaggtg ctatttgcag tgcgtctagt
      121 ttgcgcacct acgttgtact ggctcaaaat acggccggtc gttaatagca atgggaactt
      181 tcggtttgag cgttcttcag ttggcacaaa aggagtttca ataaataagc ctttaccgcg
      241 aacaaaccca ttcagatgca tgatcggggt accatcaggt gcggcatcat ttactggcca
      301 ttgcaaactg ccaacacgat ccagcaattc aaaacttacg ttcttgaatg ttggcgtcat
      361 gaccgcaatt tcatccatga tttgagctgc attgtcatag tgcattgggt aacccattgc
      421 agttgccaac ttagctacga tttcccactc ttgtaatcca ttcttcggcg ccataactgg
      481 acgaacgcga ttgatccggc gttcggcatt agtgaaagtg ccgtcttttt caaggaaaga
      541 agtgcctggg aaaaatacat gagcaaagtt tgctgtttcg ttaatgaaca aatcttgaac
      601 aacaactaga tccattgcag aaagtgcagc tttcacatgg tttgtgtttg gatctgactg
      661 ggcaatgtct tcgccatgaa caaaaattcc acggaagtca cctgatatcg cgttatccaa
      721 catgttagga atacgcattc ctggttcagg aagaattttt acatcccaaa ctgattcaaa
      781 ttgttcacgg actgcgtcgt cggatacatg tcgatatcca gaaagttcgt gtgggaatga
      841 acccatgtca caagcgcctt ggacgttatt ttgtccgcgc agtggattaa ctccaacgcc
      901 ctcacggccg atgtttccgg tggccatcgc taggtttgcc atacccataa ccattgttga
      961 tccttggcta tgttcggtaa cacctaagcc gtaatagata gcaccgttac caccagtagc
     1021 aaataatctg gctgcttggc gaatttcatc tgccggtaca cctgactgag cttgcacaac
     1081 ttctgggcta ttttccggaa gcgcgatgaa attcatccag cggtcaaatg cttcaacatc
     1141 gcagcgttca ttgatgaatt ctttagctac taatccttcg gtgacaatta catgagcaag
     1201 tgcgttgatg attgcaacat ttgtgccagg ttgaagttgt aagtggtgct gggcttttac
     1261 tcgcgcggat tcaactaaat caatctttcg tgggtccacc acaattaact tggcaccctg
     1321 acgtaaccga cgttttagtc gtgaggcgaa tactgggtgt gcatctgttg ggttggcacc
     1381 aattagcaag attacatctg atttacctac cgacttaaag tcttgggttc cagcagatgt
     1441 accaaatgtt gaactcaaac caaaacctgt tggtgaatgg caaacccggg cgcaggtatc
     1501 gacattgttg ttaccaaatg ccgcgcgaat taacttttgc acgacgaaaa tttcttcatt
     1561 tgtgcagcga gaggaagtta ttccaccaat tgcgttatca ccaaacttgg cctgaatttc
     1621 tttgaactta gcagcggtgt aattgattgc ttcatcccat gaaacttctt gccagtcatc
     1681 ggttattttc tcgcgaatca tcggcgaaag aactcggtct ttatgggttg cgtagccaaa
     1741 agcaaaacga cccttaacgc acgagtgacc ttcatttgca ccaccagttt tagctggcac
     1801 catccgaact aattcatcgc cacgaagttc ggcattgaaa gtacaaccaa caccgcaata
     1861 agcacatgtc gtttgaactg ttcgagttgg catacccagc tggatgacgc ttttctcttc
     1921 aagagttccg gttggacaag cttgcacaca tgcgccacat gaaacacatt cgctggataa
     1981 gaaattaagt ccgcctggac ttacttttga attaaagccg cggccttcaa tagttagcgc
     2041 aagtgttcct tggatttgat cacaggcacg gacacagcga ttacaaacaa tgcacttact
     2101 accttcgaaa tcaaaatatg gattacttgt atcttttggc gcatccagat gattctcgcc
     2161 gtcatagcca tagcggacgt cacgtagccc aacaacacct gccatgtctt gcaattcaca
     2221 atcaccgttt gctgcgcaag ttaaacactc aagtgggtga tcagaaatat aaagttccat
     2281 tacgttgcgg cgaagtttgc ttaactttgc agtttgtgtg cgaactacca tgccatcttc
     2341 ggctggagtt gtacacgaag ctggtgttcc tttacggcca tcaatttcaa ctagacacat
     2401 ccggcatgaa ccgaaaggtt caagtgaatc cgtggcgcaa agttttggaa ttgagcaacc
     2461 attctcacca gctgctcgca taatcgaagt tcccgctggc acagttactg gtgcgccatc
     2521 aatcaacaaa gtaacttgct gatctgagtt tgactttggt gtgccaaaat ctggttcgcc
     2581 aataccgttg cggattggcg ctattccctg ggtaaacatg tccctcggat caaatagcag
     2641 tgtcatggct aactcctgat tccaaagtca gatggatata acttaagcgc actgcgaact
     2701 ggaagtggag tcaacccgcc cattgcacat agtgaaccat ctgtcattac ttcacatagg
     2761 tcttctagta actctaaatt ctgggcttgg tcttctccag caactatttt gtcgattact
     2821 tcaaccccac gggttgaacc gatccgacaa ggtgtgcatt ttccgcaaga ctcttcagcg
     2881 caaaattcca tcgcgaatct ggcttgctcg gccatgttta ctgtgtcatc aaaaactacg
     2941 actccgccat ggccaagcat tccacccgct gcggccaacg actcgtatgc cattggaaca
     3001 tcccaagccg atttatctaa ataggcaccg agggggccac cgacttgaac cgttttaatc
     3061 gggcggccgc tatgtgtgcc gccaccaaag tcatcaacta actggccaag cgtgattcca
     3121 aaagctgtct caacaattcc acctcttgca atgt
//
LOCUS       DBNHVN010000362         2646 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_1039563, whole genome shotgun sequence.
ACCESSION   DBNHVN010000362 DBNHVN010000000
VERSION     DBNHVN010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1039563"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="AC16Y1_06840"
     CDS             complement(<1..1158)
                     /locus_tag="AC16Y1_06840"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR5832212.1"
                     /translation="MSDYALLVTALFLVIVSGVLVASEIAITRVSKVTIEQLKKDGVK
                     KAAKLFDLLDDRTPYVNALLFAHLALSTIAIVLVTDGLIGIFGDNLFAVGLSAGIMIT
                     VGYVFLGVAPQTLGRQKPEQIALATLGLARFITVFLEPLSRLFISLGNAITPGKGFRE
                     GPFSTQAELRELVDMAGADSVIEDEERQMIHSVFELGDTIAREVMVPRTEMVWVESSK
                     TLRQAMSLGLRSGYSRIPVVGESIDDIVGVVYLKDIAKRTFEHSESQTTERVSDLMRP
                     AYLVPDSRSVDELLRDMQAARTHLAVLVDEYGGTAGLVTIEDVLEEIVGEISDEFDVA
                     APDIEDLGAGDYRVSARLHIDDLAELLEIEIDEEGVDTVAGLMAKRLGIVPI"
     gene            complement(1151..1627)
                     /gene="ybeY"
                     /locus_tag="AC16Y1_06845"
     CDS             complement(1151..1627)
                     /gene="ybeY"
                     /locus_tag="AC16Y1_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR5832213.1"
                     /translation="MSIDVANESEIELDEKEIADQAAFMLRKLHINPGAELSILLVDE
                     VAMASLHEKFMDEPGSTDVLSFPMDELRAGTPTQLSAEGILGDVVICPQVAIRQAGTA
                     GHSMEVEMRLLLTHGILHLIGHDHAESEEHKVMFGLQTQLLLDWEVERNRRGKTDV"
     gene            complement(1630..2610)
                     /locus_tag="AC16Y1_06850"
     CDS             complement(1630..2610)
                     /locus_tag="AC16Y1_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014179737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR5832214.1"
                     /translation="MTISAAPIHSVVVVPTSTPMVSVVGTRDELVKVIEKSFPDVVVL
                     VRGNEFTLDGPQESVKLIEQLISEMLVVLRTGQGLTPDSVERSIAMLRSDIRPADVLT
                     ANIISNRGKAIRAKTVNQKKYVDAIDQNTIVFGIGPAGTGKTYLAVAKAVQALQSKQI
                     SRIILTRPAVEAGERLGFLPGTLSEKIDPYLRPLYDALHDMVDADSIPRLMTSGTIEV
                     APLAYMRGRTLNDAFIILDEAQNTSPEQMKMFLTRLGFGSTMVITGDVTQVDLPSGTS
                     SGLRVVREILTGIDDVQFCELTSSDVVRHKLVGQIVDAYSKFDDAKSRKR"
BASE COUNT          747 a          650 c          513 g          736 t
ORIGIN      
        1 aatcggcacg atacctaatc gctttgccat taaacctgca acagtatcta cgccttcttc
       61 gtctatttca atttccagaa gttcagcaag gtcatcaata tgtaaccgtg cacttactcg
      121 gtagtcacca gcacctagat cttcaatatc aggcgccgca acgtcgaatt catcggatat
      181 ttcgccaaca atttcttcca ggacatcttc gatagtcact aagcccgctg tgccaccata
      241 ctcatcgacc agcacagcta agtgcgttct agctgcctgc atgtctcgca ataactcatc
      301 aaccgagcgt gaatcaggaa ctaaataagc tggacgcatt aaatcagaaa ctcgctctgt
      361 tgtttgtgat tcactatgct caaaagtgcg cttggcaatg tccttgagat aaacaacacc
      421 aactatgtcg tcaatacttt caccaacgac tgggatacgc gagtagccag atcgaagccc
      481 cagtgacata gcctgtctaa gagttttcga actttcgacc caaaccattt cagtgcgggg
      541 caccattact tctcgtgcaa ttgtgtcacc aagctcaaaa actgagtgaa tcatctgacg
      601 ttcttcatct tcaatgactg aatctgctcc agccatgtcg actaattcgc gcaattcagc
      661 ttgagtggaa aatgggccct ctctaaatcc ctttcctggt gtaatcgcat tacccagcga
      721 aatgaataac cggcttagtg gttctaaaaa tacggtaatg aatctagcta atccaagtgt
      781 ggctaaggca atttgttctg gcttttgccg ccctaatgtt tgcggcgcta cacctaaaaa
      841 cacataacca accgtgatca taatccctgc agaaagtcca actgcaaata aattatcgcc
      901 aaaaattcca atcaagccat cagtaaccag cacaattgca attgtggata gggctaagtg
      961 agcgaacagc aacgcattaa catacggcgt acgatcatca agtaaatcaa aaagtttggc
     1021 tgcttttttt acgccatctt ttttcagttg ttcaattgtt actttactta ctcgtgtaat
     1081 tgctatttcg gaagcaacta atactcctga aacaatcact agaaataacg cagttactag
     1141 caatgcataa tcagacatca gttttacctc ggcgattgcg ctcgacttcc cagtccaaaa
     1201 gtaattgagt ttgtaaacca aacatcactt tgtgttcttc agattccgca tggtcatgac
     1261 caatcaaatg caaaataccg tgagttagta gtaggcgcat ttcgacttcc atactgtgtc
     1321 cggccgtacc tgcttggcgg atcgcaactt gtgggcaaat taccacgtcg ccaaggattc
     1381 cttcggcgct aagctgcgtt ggagttccag ccctaagttc atccatcgga aaactcaata
     1441 cgtcggtact tccaggctca tccataaatt tttcgtgcag cgaagccata gcaacctcat
     1501 caacaagcaa aattgaaagt tcagctcctg gattgatgtg caacttgcgc agcatgaaag
     1561 cagcttgatc tgctatctct ttttcatcaa gttcaatttc ggattcgtta gcaacgtcaa
     1621 tcgacatact tagcgtttcc tgctttttgc atcatcaaac ttgctatagg catccacaat
     1681 ctgtccaact aatttatgtc gaactacgtc gcttgaagtt agctcacaaa actgcacgtc
     1741 atcaattccg gtaaggattt cccggaccac gcgtaaacca ctagatgttc cactaggtaa
     1801 atcaacctgg gtaacgtcgc cggtaattac cattgttgaa ccaaaaccta aacgagtcaa
     1861 aaacattttc atttgctcag gtgaggtgtt ctgcgcctca tccagaataa tgaatgcatc
     1921 gttaagagtt cgcccacgca tataggcaag tggcgcaacc tcgattgttc cactagtcat
     1981 gagacgcggg attgaatcgg catcgaccat atcatgtagg gcgtcatata gaggccgaag
     2041 gtatgggtca attttttcgc ttaatgtgcc aggtaaaaat ccaaggcgct cgcctgcttc
     2101 tactgcaggt ctagtcagaa taattctaga aatttgttta ctttgcaatg cttgaactgc
     2161 cttggcaacg gctaggtaag ttttgccggt accagcgggg ccaataccaa aaacaattgt
     2221 gttttggtca attgcatcaa catacttttt ttgatttacc gttttagctc taattgcttt
     2281 gccacgattt gaaattatgt ttgcggtcag cacatctgct ggcctgatat cacttcttaa
     2341 catggcaatt gaacgctcta cggaatcagg tgttaatcct tggccggtgc gcaacacaac
     2401 cagcatctcg ctaattaatt gttcaattaa tttgacgctc tcttgcggtc catccaaagt
     2461 aaattcgttg ccacgaacaa gcaccacaac atctggaaat gacttttcta tgactttaac
     2521 taactcatcg cgcgtgccaa ctacagaaac cataggtgtg gaagtgggca caacaactac
     2581 tgaatgaatt ggtgctgcgg aaattgtcat taatccaatt ctaaatgatt acccgggagg
     2641 ccaatt
//
LOCUS       DBNHVN010000363         1645 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Actinomycetes bacterium isolate MAG05105
            SRR25158351_k127_593708, whole genome shotgun sequence.
ACCESSION   DBNHVN010000363 DBNHVN010000000
VERSION     DBNHVN010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414513
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetes bacterium (soil metagenome)
  ORGANISM  Actinomycetes bacterium
            Bacteria; Bacillati; Actinomycetota; Actinomycetes.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:25:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,374
            CDSs (total)                      :: 1,344
            Genes (coding)                    :: 1,337
            CDSs (with protein)               :: 1,337
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 4 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Actinomycetes bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_593708"
                     /isolate="MAG05105"
                     /isolation_source="soil"
                     /db_xref="taxon:1883427"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="AC16Y1_06855"
     CDS             <1..370
                     /locus_tag="AC16Y1_06855"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR5832215.1"
                     /translation="QVGVASVREFLAKADLVLANEDEFALLADPEWLAGVENLIVKLG
                     PSGAKWLVAGQEVNKVSANKVKVLDTTGAGDSFLAGLISNLTNHADWFAISTEDRILA
                     LTRAIEVAEENISKIGAGPI"
     gene            404..859
                     /locus_tag="AC16Y1_06860"
     CDS             404..859
                     /locus_tag="AC16Y1_06860"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SixA phosphatase family protein"
                     /protein_id="HIR5832216.1"
                     /translation="MSRVYVLRHAQAQSGLKDHSRELTQYGRLQAQMMGQWLRLNLHN
                     LDLAIVSSSRRTVQTFDGLDLGIDSVIDDRAYNADATQLIELVNELGVDHESVLLIGH
                     NPGVTDLVNLAGYPQPLSPCTCVILEFSESVSVFDPQAAKVELWHQARP"
     gene            complement(863..1363)
                     /locus_tag="AC16Y1_06865"
     CDS             complement(863..1363)
                     /locus_tag="AC16Y1_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5832217.1"
                     /translation="MKIGIAGSFLFLAVALAGCTAQSDSNGLLGETSLSNTSNKVIPP
                     LQSDNSSVDCSGWTQDATTYIRNQGLLTIDKTIYGVATFSCGAPDSELSTEYVEAFVF
                     SNGVWAGAGIVPGPRLQFMTTQPCTSGEQVTCSALQIIGPDEEVPGTIVIFQEGSDLA
                     WRFDPN"
     gene            1463..>1645
                     /locus_tag="AC16Y1_06870"
     CDS             1463..>1645
                     /locus_tag="AC16Y1_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5832218.1"
                     /translation="MENADRFAVPGKKGNFLHYSGTGGPLTYEADFVVVDVETTGFSA
                     WQGDRIVELGAVRLSAD"
BASE COUNT          466 a          345 c          383 g          451 t
ORIGIN      
        1 ccaagttggt gttgcaagtg ttcgggagtt tttagctaaa gcagatttag ttctggcaaa
       61 cgaagacgaa tttgcgctac ttgctgatcc agaatggtta gctggcgtag aaaacttaat
      121 agtaaaactt ggtccatcgg gtgctaaatg gttagttgct ggacaagaag taaacaaagt
      181 ctcggctaac aaagtcaaag ttctcgatac aacaggtgcg ggcgattctt tcttggctgg
      241 gctaatttcg aatttaacca atcacgcaga ttggtttgcg atcagcaccg aagacagaat
      301 tcttgcctta actcgagcaa ttgaagtagc tgaagaaaat atttcaaaaa ttggtgccgg
      361 acccatttga ctcgagcaat ttaggcaact ctaggcttta cttgtgagcc gtgtttatgt
      421 tttgcgtcat gcccaagcac agtctgggct taaggatcat tcgcgagaat taactcagta
      481 tggcaggcta caagcgcaaa tgatggggca gtggctacgg ctcaatttac ataatttgga
      541 tttggccata gtgagctcgt cgcgcagaac tgtacaaact tttgatggcc tagatttagg
      601 tattgattca gtaattgacg atcgagcata taacgctgat gcgacacaac taattgaact
      661 agtcaatgaa ttaggtgttg accacgagtc agttttactt atcggacata acccaggggt
      721 taccgatctt gttaatctcg ctggatatcc ccagccacta tcgccatgca cttgcgtaat
      781 cctcgagttt tcagaatcgg taagtgtatt cgatccgcag gctgcaaaag ttgaactttg
      841 gcatcaagcc agaccctaac ctctaattag gatcgaagcg ccaagctaga tcagagccct
      901 cttggaaaat aacaattgtt ccagggactt cttcgtccgg accgataatt tgtaaggctg
      961 aacacgtgac ttgctcgcca gatgtacaag gttgtgtagt cataaattga agccgaggtc
     1021 ctggaactat tccggctccg gcccatacgc catttgaaaa aacaaatgcc tcaacatact
     1081 cggtacttaa ctcgctatcg ggcgctccgc atgaaaaagt agctactcca taaattgtct
     1141 tatcaattgt cagtaaacct tgattgcgaa tgtaagtagt tgcgtcttgc gtccaacccg
     1201 aacaatcaac tgatgagtta tctgactgca atggtggaat taccttattt gatgtgttgg
     1261 aaagtgaagt ttcaccaagt aagccatttg aatcagattg cgccgtgcag ccagcgagcg
     1321 caacagccaa aaataaaaaa cttccagcta tgccaatttt cataaagtcc agattacttt
     1381 gtgattttca taaaagttac cggctgataa ttgagtgtct gagccaccaa gtagttttat
     1441 ctactagaaa aggggtgcct cgttggagaa cgcagatcgt tttgcagttc ctggcaaaaa
     1501 agggaacttt ttgcattaca gcggcactgg tgggccatta acttacgaag ctgacttcgt
     1561 agtcgttgac gtagaaacaa ccggattttc tgcttggcaa ggagaccgaa tcgtagaact
     1621 tggtgcagtc aggcttagtg ccgat
//
