LOCUS       DBNHVU010000001         6185 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1486887, whole genome shotgun sequence.
ACCESSION   DBNHVU010000001 DBNHVU010000000
VERSION     DBNHVU010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1486887"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="AC16Y7_00005"
     CDS             <1..651
                     /locus_tag="AC16Y7_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020809838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HIR5707795.1"
                     /translation="DPLEAEPAIELVRVRPGSALPGDAGLVILPGSKCTRADLEALRG
                     AGFDIDIAAHVRRGGAVLGLCGGFQILGRVVHDPDGIEGAPGSTAGLGLLDVETTLTG
                     EKRLEPAQGETFDGAPFSGYEMHMGETLGADCTRAFAQLTDGAKDGAMSPDGRVIGTY
                     VHGLFADDRQRGAWLARFAAGTPQIAYDSSVDAILDRLAEHLEAHVDIDRLLTISR"
     gene            complement(635..1600)
                     /gene="cbiB"
                     /locus_tag="AC16Y7_00010"
     CDS             complement(635..1600)
                     /gene="cbiB"
                     /locus_tag="AC16Y7_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HIR5707796.1"
                     /translation="MAAPLALLALLIELVVGYPDAIMKKIGHPVTWMGHLIGFLDKSL
                     NKDGAPAESRRRTGAIALFVLILVVGAIAFIAETALLLLPFGLIFAGAACSTLIAQRS
                     LYMHVANVADALESSNLVKARKEVAHIVGRDTAELDEAGVARAAIESLAENFSDGVVS
                     PVLWIWLAGLTGGAVYKAINTADSMIGHRSERYESFGKPAAQLDDLVNLPGSRASGLL
                     IVAAAALTGGASASGAWNTMVRDAGKHASPNAGYPEAAMAGALDVSLGGPHRYDGEET
                     DGAWLGEGRRDLKAADVQAALDVYGRADGLLIALVFVLVAVTAIL"
     gene            1599..2609
                     /gene="cobD"
                     /locus_tag="AC16Y7_00015"
     CDS             1599..2609
                     /gene="cobD"
                     /locus_tag="AC16Y7_00015"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HIR5707797.1"
                     /translation="MIPPNAYAPLVHGGDLNAARRLFPDAPEPFLDLSTGINPNPYPI
                     PPLSPQVFAQLPQEDTLAKLLACAAAAYGAPDESNVVPAGGMQILVTLIAGAAPPGLT
                     RIVGPTYAEHARALVLAGHDVETVPDLAPLGEAALSLVVNPNNPDGRVFAREDLLAIA
                     ARQRKSGGMLIVDEAFMEIGPREESLAGDVEEANVIVLRSLGKAYGLAGLRLSVALAA
                     TTAAARLRAALGPWPLSGPALEVGAQALSDMTWLTATRAALAKDGKRLDELLETAGLA
                     RVGGTPLFTLVRHDDAPQVFDRLGRAGIFVRRFAEEPTWLRFGLPGAEADWQRLEVAL
                     RG"
     gene            complement(2606..2827)
                     /locus_tag="AC16Y7_00020"
     CDS             complement(2606..2827)
                     /locus_tag="AC16Y7_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707798.1"
                     /translation="MAAESLVQMLEEIAIGLKAGDEEVYGWLRDQLKESGGSEISVEL
                     AMGVIARLLPEEAQGNLELAEHYSRRWAH"
     gene            complement(2974..3726)
                     /locus_tag="AC16Y7_00025"
     CDS             complement(2974..3726)
                     /locus_tag="AC16Y7_00025"
                     /inference="COORDINATES: protein motif:HMM:NF018597.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin-like domain-containing protein"
                     /protein_id="HIR5707799.1"
                     /translation="MAALLVTAAFLKACGGGSVPREDRAEWRDRAEAQCMASGRVRKS
                     SYVSQVSMISGPSSCGIRAPLKVSAALGGRVRITPTATIGCPVTASLDRWIHKSVQKA
                     AYRQFGKPVVEIKQISSYSCRGRNGRKRGPLSEHSYGNAIDIAGFRLSDGRQITVVKG
                     WWRGSRREKAFLRDVFAGACDEFYTVLGPGSDRHHYNHFHLDLLVTNVSKGRHYCRPK
                     PRRGLFAEAGGLAADPVSTASIKPIPFTGPPD"
     gene            complement(3848..4489)
                     /locus_tag="AC16Y7_00030"
     CDS             complement(3848..4489)
                     /locus_tag="AC16Y7_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707800.1"
                     /translation="MSDSSPWLILIALGAFHGLNPAMGWFFAASLGLFRKSRSVILLS
                     LIPIALGHAAAIAVVVFAAIGLGFTIDADTFRILSGALLIAWGLYHWFFGHQHHLRIT
                     AATGLTGIGVWSFLMAMAHGAGMMLIPVLMPMGHVHTHMGGHGGHMVSTDSFGVAALG
                     VIVHTLAMLIVTGCIAILVYQSAGLEILKRRWINLDLAWTLALIGMGLWLLFA"
     gene            complement(4585..>6185)
                     /gene="ligD"
                     /locus_tag="AC16Y7_00035"
     CDS             complement(4585..>6185)
                     /gene="ligD"
                     /locus_tag="AC16Y7_00035"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HIR5707801.1"
                     /translation="QEHIDTPHGAIRYFAFDLLFLDGKDLRREPLTTRKAKLRDLLAA
                     KGVSDFLIYSDHVVGSGPAFFETASAAHLEGVISKKADGPYRSGRGRDWLKTKCIKSQ
                     KFVIGGFSKSDVRGKPFASLLLGRFDGDTFNYAGKVGTGFKAQDYAALAKRFKPLIRK
                     TSPFVSVPASESKGAVWLSPKLVCMINYAEWTRDGRLRHPSFQGLRDDKPARDVTREA
                     SNNPETAMAAKAKTAARNVQFGEVTLTSPDRVLYPEIGFTKLDLARYYDGIAKAMLPY
                     AASRPISLVRCPQGVDGERFFQRHAMKGMSRAIKQIPIPGGQSKKPYLYIDDVDGLYG
                     LVQIAALEIHDWGVSLKALDKPDRLVFDLDPDEELDFAVMKAAAAEVRGFLDDLGLTS
                     FLKATGGKGLHVVAPLTPKAGWDEVKTFSKAVADALVVARPDRYTANPLKRTRVGKIF
                     VDYLRNQRGSSAICNFSTRAKRQATVATPLRWDELKRLKSGAPYTVESVPARLKRLKS
                     DPWEDFFSTRQSITAKARKALGLD"
BASE COUNT         1138 a         1980 c         1979 g         1088 t
ORIGIN      
        1 gatccgctcg aggcggagcc cgccattgag ctcgtgcgcg tgcgccccgg cagcgcgctg
       61 cccggtgatg cgggcctcgt catcctgccg ggctcgaaat gcacgcgcgc cgacttggag
      121 gcgctgcggg gcgccggctt cgacatcgac atcgccgcgc atgttcgccg cggcggtgcg
      181 gtgcttggcc tgtgtggcgg cttccagatt cttggccgcg tggtgcacga ccctgacggc
      241 atcgagggcg cgcccggcag cacggcaggc cttggcctgc tcgacgtcga gacaaccttg
      301 accggcgaga aacgcctgga gcctgctcag ggggagacct tcgacggtgc acccttctca
      361 ggctacgaga tgcatatggg cgagacgctg ggtgcggatt gtacgcgggc cttcgcgcag
      421 ctcaccgacg gagccaaaga tggtgcgatg tcgccggatg gccgcgtaat cggcacctat
      481 gtccatggtc ttttcgccga cgaccgccaa cgcggcgcgt ggctggcacg gttcgccgcc
      541 ggcacgccgc agattgccta cgactcatcc gtcgatgcga tcctcgatcg tctcgccgaa
      601 catttggaag ctcatgtcga tatcgatcgc ctgctcacaa tatcgcggta actgcgacca
      661 gcacgaacac aagggcgatc agcagcccgt ccgcgcggcc gtagacgtcg agtgcggctt
      721 ggacgtcggc tgccttgaga tcgcgccgcc cctcgccgag ccacgcgccg tcggtctcct
      781 cgccgtcata gcggtgcggg ccgccgagag acacgtcgag cgcgccggcc atggccgctt
      841 ccgggtagcc ggcattgggc gaggcgtgtt ttccggcatc gcgcaccatc gtgttccagg
      901 cgccgctggc cgacgcaccg cccgtcagcg cggccgctgc gacgatcaga agtccggacg
      961 cccgcgagcc cggcagatta acaaggtcat ccagctgcgc cgcaggcttg ccgaaacttt
     1021 cataccgctc gctgcggtgt ccgatcatgc tgtcggcggt gttgatggcc ttgtagacgg
     1081 cgccgcccgt cagcccagcg agccaaatcc acaacaccgg cgacacgacg ccgtcggaga
     1141 agttctcggc gagactttcg atcgccgcgc gcgccacacc agcctcgtca agctctgccg
     1201 tatcgcgtcc gacaatatgc gcgacctcct tgcgggcctt gaccagattg ctggactcaa
     1261 gcgcatcggc aacgttggcg acatgcatat agaggctgcg ctgcgcgatg agcgtgctgc
     1321 aggcggcccc ggcgaagatc agcccgaacg gcaacagcag cagcgcggtc tcggcgataa
     1381 aggcgatggc gccgaccacg aggatcagga cgaagagcgc gatggcaccc gtccggcggc
     1441 gcgactccgc aggcgcgccg tccttgttca ggctcttgtc gaggaaaccg atcaaatggc
     1501 ccatccaggt gacgggatgg ccgatcttct tcatgatcgc gtccgggtaa ccgacgacga
     1561 gctcgatcag caaagcgaga agcgctagcg gtgcggccat gatccctccc aacgcctatg
     1621 cgccgctcgt tcacggcggc gatctgaacg ccgcgcggcg cctgttcccc gacgcgcccg
     1681 agccgtttct cgacctatcg acggggatca atcccaatcc ctaccctatt cccccgcttt
     1741 cgccgcaggt ttttgcgcag ctcccacaag aggacacgct cgcaaagctg ttagcctgtg
     1801 cagcagccgc ttatggcgcg ccagatgagt ccaatgtggt tccggccggg ggcatgcaga
     1861 tcctcgtgac cttgattgca ggagcggctc caccaggcct tacccgtatt gtcggaccga
     1921 catacgcgga gcatgcgcgc gcgcttgttc tcgccggtca tgacgtcgag acggtgcctg
     1981 accttgcccc gctcggcgag gccgcgcttt cactggtggt caatccaaac aaccccgatg
     2041 gccgggtctt tgcccgcgaa gatctgcttg ccattgcggc gcggcaacgc aagtccggcg
     2101 ggatgttgat cgtcgacgaa gcgtttatgg agattgggcc gcgcgaagag agtctcgccg
     2161 gtgacgtcga ggaggccaac gtcatcgttc tgcgctctct tggcaaagca tatgggctgg
     2221 cgggtcttcg tctcagtgtc gcgcttgctg caacgacggc ggccgcgcgt cttcgcgccg
     2281 cgctcggccc ctggccattg tcagggcccg cgctcgaggt cggcgcgcaa gcattgtccg
     2341 acatgacttg gctgaccgca acgcgcgccg cgctggcgaa agacgggaag cgtctcgatg
     2401 aattgctgga gaccgcaggc ttggcgcggg tcggcggcac gccgctcttc acgctggtcc
     2461 ggcacgatga cgcaccgcaa gtcttcgatc gtctgggacg tgcagggatc ttcgtgcgcc
     2521 ggttcgccga ggaaccaaca tggctgcgct tcggtcttcc cggcgcggag gccgactggc
     2581 aacgtctgga agtcgcgctg cgcggttagt gcgcccagcg gcggctgtag tgctcggcga
     2641 gctcgagatt gccttgtgct tcctcaggca ggagcctggc gatcacaccc atggcaagct
     2701 cgacggaaat ctcgctgccg ccggactctt tgagttggtc gcgcagccag ccatagactt
     2761 cctcatcgcc ggccttgagc ccgatggcga tctcttcaag catttgcacc aacgattcgg
     2821 ctgccatcgc gcccctccaa tcgaaccaag cccaaaggct tatggctgcg gtcttgcgtt
     2881 actcccggtg cgaatccccg aagaggacca tatcgccaat tcgccaggcg cgacgttgcc
     2941 cgcaggccac agtggagggg tttctggtga agattagtcc ggcgggccgg tgaaggggat
     3001 cggcttgatg gaggcggtgg agaccgggtc ggccgccaga ccgcctgcct ccgcgaacaa
     3061 cccgcggcgg ggcttgggcc ggcagtaatg gcgtcccttg gacacgttgg tcacgagcag
     3121 gtctaagtgg aagtgattgt agtggtgacg gtcgctgccc gggccgagca cggtgtagaa
     3181 ctcatcgcag gcgccagcga agacgtcccg gaggaaagcc ttctcccgcc gagagccgcg
     3241 ccaccagccc ttgaccacgg tgatctgcct gccatccgaa aggcggaacc cggcgatgtc
     3301 gatggcgttg ccgtaggagt gttcggaaag cggaccccgc ttgcggccgt tccgccctcg
     3361 gcagctatag gatgagatct gcttgatctc gaccaccggc ttgccgaatt ggcgataggc
     3421 agccttctgc accgatttgt gaatccagcg atcgaggctt gccgttaccg ggcagccaat
     3481 ggtcgcggtc ggagtgatgc gcacgcgacc ccccaaggca gctgacacct tgagcggtgc
     3541 ccggatgccg caagagctcg gcccgctgat catggagacc tggctcacat aggacgattt
     3601 gcgcacgcgg cccgaggcca tgcattgcgc ctccgcccgg tcgcgccatt ccgcgcgatc
     3661 ctcccgcgga acgctgcccc cgccacatgc cttgaggaag gcggccgtca ccagaagtgc
     3721 ggccacaaac gcaatacgaa cacccaccat ggacgccaca atggggccaa gggtctgaaa
     3781 aaatactcaa tggcaggagt gcggagcgcc tagatatgcg gcgcgggcgg cacaaaaaga
     3841 gaacgcatca ggcaaacaac agccagagcc ccatgccgat cagcgccagg gtccaggcga
     3901 gatcgagatt gatccagcgc cgtttcagga tctcaagccc tgccgactgg tagaccagga
     3961 tcgcgatgca gccggtcaca atcagcatgg cgagggtgtg gacgatgacc cccagtgcgg
     4021 caactccaaa cgagtccgtc gacaccatat gaccgccgtg cccgcccata tgggtgtgca
     4081 catgccccat cggcatgagc accgggatca gcatcatgcc ggcgccatgc gccatggcca
     4141 tgaggaacga ccacacgccg attcctgtaa gccccgtcgc cgccgtgatg cggaggtgat
     4201 gctgatggcc gaagaaccag tgatagagcc cccacgcgat tagcagggca cctgagagta
     4261 tgcggaacgt gtcggcatcg atagtgaacc cgagcccgat cgccgcgaat accacgacgg
     4321 caatagcggc ggcatggccc aatgcaatgg ggatcagcga aagcaggatc acgctacggc
     4381 tcttgcggaa gagcccgagg gacgcggcaa agaaccagcc catggccggg ttgaggccgt
     4441 ggaaggcgcc gagcgcgata aggatgagcc agggggacga gtcagacatg aggccgcgtt
     4501 gttgacacga aaagccagcc gaatggacgt gagattgagg gcacgcgtcc ttgatcgagc
     4561 gcaaatacct cgcggcacga ggtctcagtc aaggccgaga gccttccgag cctttgccgt
     4621 gatcgactgg cgtgtgctga aaaaatcttc ccaagggtcg gacttcagac gcttgaggcg
     4681 cgcgggcaca ctctcgacag tgtaaggcgc gccgcttttt agcctcttga gttcgtccca
     4741 acgcagcggc gttgccacgg ttgcttgccg tttcgcgcgc gtcgaaaaat tgcagatcgc
     4801 cgagctgcca cgctgattgc gaagatagtc gacgaaaatc ttgccgacgc gcgtccgctt
     4861 cagcgggttc gccgtgtagc ggtcgggccg cgcgacaacg agcgcatccg ccacagcttt
     4921 ggaaaaggtc ttgacctcat cccaaccggc cttcggggtg agcggtgcga ccacatgcaa
     4981 gcccttgccg ccggtcgctt tgagaaagct cgtgagcccc aggtcgtcaa gaaagccgcg
     5041 cacctctgct gcggcagcct tcatcaccgc gaagtccagc tcttcgtccg ggtcgaggtc
     5101 gaacaccagt cggtcgggtt tgtcgagagc tttgagtgac acgccccaat catggatctc
     5161 gagcgcggcg atctgcacca gcccatacag cccgtccacg tcgtcgatat agagatacgg
     5221 cttcttcgat tggccgcccg gtatcgggat ctgcttgatc gccctgctca tgcccttcat
     5281 ggcatggcgc tgaaagaacc gctcaccgtc gacgccctgc gggcagcgca ccaggctgat
     5341 gggccggctc gctgcgtaag gcagcatggc cttcgcgatg ccgtcgtagt agcgcgcaag
     5401 gtcaagcttg gtgaagccga tttcgggata gagcacgcgg tcggggcttg tcagggtcac
     5461 ctcgccgaat tgcacattgc gtgcagctgt cttcgccttc gcggccatgg cggtctccgg
     5521 attgttcgac gcttcgcggg tgacgtcgcg cgcaggcttg tcatcgcgta ggccctgaaa
     5581 gctcggatgg cgcaagcgtc cgtcgcgcgt ccactccgca tagttgatca tgcagacgag
     5641 cttcggacta agccacaccg cgcctttgct ctccgacgcc ggcacgctca cgaacggcga
     5701 agtcttgcgg atgagcggtt tgaagcgctt ggcgagggcc gcatagtcct gcgccttgaa
     5761 accggtgccg accttgccgg catagttgaa cgtgtcgcca tcgaacctgc cgagcaagag
     5821 cgaagcgaaa ggcttgccgc gcacgtccga cttggaaaag ccgccgatga cgaacttttg
     5881 gctcttgatg catttggtct tgagccaatc ccggccgcgg ccggagcggt aaggtccgtc
     5941 cgccttcttc gatatgacgc cttcaagatg cgccgcggac gcggtctcaa agaaggcggg
     6001 tcccgatccc acgacgtgat cggagtagat gagaaagtcc gagacgccct tggccgccaa
     6061 caggtcacgc agctttgcct tacgtgtcgt gagcggttct ctccgcaaat ccttgccgtc
     6121 gagaaacaga aggtcgaacg cgaagtagcg gatcgcgcca tgcggcgtat cgatgtgctc
     6181 ttgaa
//
LOCUS       DBNHVU010000002        15542 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_75462, whole genome shotgun sequence.
ACCESSION   DBNHVU010000002 DBNHVU010000000
VERSION     DBNHVU010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 15542)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15542)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15542
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_75462"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="AC16Y7_00040"
     CDS             <1..638
                     /locus_tag="AC16Y7_00040"
                     /inference="COORDINATES: protein motif:HMM:NF012697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707802.1"
                     /translation="PHYFKPTEFVIAEGLLGYTTRAMRDCYDVKIYLDPEDDLRIKWK
                     IHRDTSKRGYGLEEVVASLERRKQDSPNFIHPQRTFADIVIRFQSPKGANGSDTQLDV
                     RHLLRPTLPHPDFTPLFDGSSNAGLHLELVRDKDGKPVDVLEINGNISDKRAERIEDL
                     LWNLIPEAGHLRDQVGRIDVANGANKSHPLALTQLLVGYHAVKAAMGVRAY"
     gene            663..2678
                     /gene="tkt"
                     /locus_tag="AC16Y7_00045"
     CDS             663..2678
                     /gene="tkt"
                     /locus_tag="AC16Y7_00045"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR5707803.1"
                     /translation="MSEALAQDQALSGASHDDMANVIRALAMDAVQKAASGHPGMPMG
                     MADVATVLFTRFLKYDAAHPEWPDRDRFVLSAGHGSMLLYALLYLTGYPDMDIEQLKN
                     FRQFGARTAGHPEYGHAPGIETTTGPLGQGIGNAVGMALAERLLNQRYGDDIVSHYTY
                     CIAGDGCLMEGLSQEAISFAGHLKLGRLIVLFDDNEISIDGGTNLTLSDDQLARFRAS
                     KWHVATVDGHDPEAVALAIENARGVTDQPSMIACRTVIGYGAPNKAGTAATHGAPLGE
                     DEIAGARQELGWPHASFEVPDAVLESWRAAGMCGRADYDKWMEAAGRLDAASRARLTD
                     PIDAKVGEDIIRAMAGIKTDFAAEGAKLATRQSSQKVLERLMPVVPGLIGGSADLTGS
                     VGTLTKQHGIVKPDDFAGNYIHYGVREHAMGASMNGLALHGGIVPYAGTFLVFSDYCR
                     PSIRLSALMEQRVVYVMTHGSIGLGEDGPTHQPVEQLAALRAMPQLHVMRPADSVECA
                     ECWELALLSKDRPTIMTLTRQGLPLLRTTPTDDNLSAKGAYVLIEPEGGRDVTVLATG
                     SEVSLAVNAAKMLEEQGIKAAVVSMPCWELFEAQDEGYQSAVLGTTPRVGVEAAVGFG
                     WDKWLGENGKFIGMRGFGASAPAQELYKHFEITPEAVVAAARDLISN"
     gene            2692..3705
                     /gene="gap"
                     /locus_tag="AC16Y7_00050"
     CDS             2692..3705
                     /gene="gap"
                     /locus_tag="AC16Y7_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR5707804.1"
                     /translation="MANTRVAINGFGRIGRLTFRAFLEAGRKDLDFVAINDLGSADMN
                     ALLLEFDSVHGRLPANVSVSDGRLKADGQDVAVLSEADPEKLPWGELGVDIVMECTGR
                     FTKRETAAQHLEAGAKRVLVSAPCSGADLTVVYGVNDDKLTEKDHVVSNASCTTNCLA
                     PVAEVLHKAVGIERGYMTTIHAYTGDQRTVDTLHKDPRRARASAVNLIPTSTGAAKAV
                     GLVLPELQGKLDGASIRVPVPNVSLIDLTFQSSRPTTVEEINDAMTKAASKAPLKGVM
                     GINEQPLVSSDFNHDPLSSVFDATGTQVIDGTFCRVVSWYDNEWGFSNRMNDTAVAMG
                     RLI"
     gene            3702..4955
                     /locus_tag="AC16Y7_00055"
     CDS             3702..4955
                     /locus_tag="AC16Y7_00055"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR5707805.1"
                     /translation="MSDDAGMGGVDLSRIKTIDGLDVKGKRVLMRADLNVPVENGHVA
                     DATRIERALPTIEALRSGGAKLVIMSHFGRPKGERSPETSLKPVAAKLQEMLGTRVSF
                     LHDCVGPDVEAGVNALKDGDVALLENLRYHKGETANDAGFAKQLAALGDIYVDDAFSC
                     AHRAHASVEAITHLLPSYAGKSMMAEIEALASALHNPKRPVMAIVGGAKVSTKIDVLT
                     NLVQRMNVVVVGGGMANTFLYAQGVDIGNSLCEPDLKDIALDIIDKAKVSGCEIMLPV
                     DQVVAKELAPGAETVVCDTGTVPAGMMILDVGPKSIARLKEKLGEMATLLWNGPLGAF
                     EITPFGEGTFALAREAGRLSKDGKLITIAGGGDTVRALNTTGAAPDFTYISTAGGAFL
                     EWLAGEQLPGVVALAGGDTKHSAVA"
     gene            4966..5640
                     /gene="rpe"
                     /locus_tag="AC16Y7_00060"
     CDS             4966..5640
                     /gene="rpe"
                     /locus_tag="AC16Y7_00060"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008132866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR5707806.1"
                     /translation="MSDDIIIAPSILSADFAKLGEEVKAIDKAGCDWIHIDVMDGHFV
                     PNITIGPDVVKALRPHCDKVFDTHLMIAPCDPYLDAFAEAGSDIITVHAEAGPHLDRS
                     LQHIRSLGKKAGVSLTPSTPETAIEYVLDKVDLILVMTVNPGFGGQSFIPEQLKKISA
                     IRKMIGDRPIHLEVDGGVNPETAGLVAEAGADVLVAGSAVFKGGAYAKNIAAIRDAAL
                     RARDAA"
     gene            5766..6500
                     /locus_tag="AC16Y7_00065"
     CDS             5766..6500
                     /locus_tag="AC16Y7_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HIR5707807.1"
                     /translation="MDVTYFGALFAGFLSFLSPCVLPLVPPYLCFLGGTTFDQLAGED
                     ETPPHVYTTVVLSSVAFVLGFTTVFVILGATATVLGQLVLENLPILAKVAGVIIIIAG
                     LHFLGVIHIPILHREARYHADTRPAGLVGAYIIGLAFAFGWTPCIGPVLGAILAVAAG
                     EDSVRQGVSLLFVYSLGLGIPFILAAVAVRPFMNAMGRFKGHMGTIEKVLGALLVLTG
                     ILFLTNSMTLIAGWILRTFPALATIG"
     gene            6580..7389
                     /gene="thiD"
                     /locus_tag="AC16Y7_00070"
     CDS             6580..7389
                     /gene="thiD"
                     /locus_tag="AC16Y7_00070"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR5707808.1"
                     /translation="MTPRSTSIALTIAGSDSSGSAGIQADLKTFGALGVHGACVVAAV
                     TAQNTQKVEAILPVPPEFVLAQMQAVANDIDVGAIKIGMLATRDNIEAVAEGLTLFDG
                     TPVVLDPVMASHRGDPLLDESAVDTLKTMLMPCATLVTPNLFEAALLTSEEKARSVDA
                     MAEQAWMLRGLGAKAVLVKGGHLDGEAIDVLLSNDGVERLEAPRHETGNTHGTGCTLS
                     SAIAAGLAHGKPLEKAVKDAKVYVTAAIIAADEIAVGKGRGPLHHFHALWP"
     gene            complement(7402..8247)
                     /locus_tag="AC16Y7_00075"
     CDS             complement(7402..8247)
                     /locus_tag="AC16Y7_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR5707809.1"
                     /translation="MKLVRFGAPGAEKPGVLDAKGLIRDLSGEIGDLAGDALGRASLD
                     RLKAIDPETLPLVEGNPRLGPPIGVVPKFLGIGLNYRDHAEETGMDIPEVPIVFCKAN
                     SCVSGPTDPVLAPPDFQRMDYEVELAVVIGTPAKRVSKDDALDYVAGYCICNDVSERS
                     LQKGGPGEWMKAKSYDSFGPLGPWLVTTDEVTDPQALDLTMDLNGERMQTGNTETMIF
                     TVADLVSYISTYMTLMPGDVITTGTPPGVGMARKPRVFIKPGDEMVLHVQGLGEQKLK
                     VVADG"
     gene            8365..8868
                     /locus_tag="AC16Y7_00080"
     CDS             8365..8868
                     /locus_tag="AC16Y7_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707810.1"
                     /translation="MRLRLRSPRLGTLIAVFLANALTVLSALPASANPYQQLDGAWTG
                     SGTVTPLKGAPEKVSCNTTYKTEGSVVRQTVRCASASHAFAGNLELTYEGGRIRGAWR
                     EQVHGASGDVSGTANGSSIRARLSGQTFSGRMRIDVDGPKHTIEIVQRDTGSGAYRPV
                     ASISLHR"
     gene            complement(9381..10250)
                     /locus_tag="AC16Y7_00085"
     CDS             complement(9381..10250)
                     /locus_tag="AC16Y7_00085"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HIR5707811.1"
                     /translation="MTNGDLRIRLEGALGSGVVDTAPLPVGFGLTGLSARLQDGRHVA
                     VKAKDNARSGPSLALEGYMLGELSRTSDLPVPQVHAAEPDLLVMDFIETDGGTITANV
                     ERDAARLIAALHATPRDGFGYARDTLIGPLHQPNPQSQCWIPFFRDERLLYMARAAKD
                     EGRLPAALYGRIETLASKLEDFLTEPPFPSLLHGDLWTGNVLVCGNRIAGFVDPAIYC
                     GHPEIELAFTTMFGTFGDAFFEAYEGMSPLEPGFHELRRDLYNLYPTLVHVRLFGGSY
                     LGRVETTLTKLGL"
     gene            10326..11156
                     /locus_tag="AC16Y7_00090"
     CDS             10326..11156
                     /locus_tag="AC16Y7_00090"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HIR5707812.1"
                     /translation="MLNTRIDDYTQPDAEQRRYRALFISDIHLGTKACQAEAFLDFLR
                     YHDAPRFYLVGDIVDFWRIRRGVYWPQAHNDVLQKLLRKVRKGTELVYIPGNHDEVMR
                     EYCGSQFGGIAIERQTIHTGADGRRYLIMHGDEFDVVVRYAKWLAFLGDWSYTAALWF
                     NTHLNAVRRWFGMPYWSLSAYLKHKVKRAVNYIGEFEQALSQEARRNGAQGVLCGHIH
                     HAAMRQVGDVVYINTGDWVESCTAVGETEEGVFEIIHWNPHTGVRTSGTPAKELEAAA
                     "
     gene            11156..12331
                     /locus_tag="AC16Y7_00095"
     CDS             11156..12331
                     /locus_tag="AC16Y7_00095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5707813.1"
                     /translation="MRILVATDAWFPKVNGVVRTYERLAQELPKLGADISFLTPAPFY
                     TLPCPTYPEIRLSLVSPSMVARHIEEARPDFIHIATEGPIGLMARGYCRKIKRPFTTS
                     YHTRFPEYVSARLPIPESWCYAFQRQFHNSGAGLFVATQSVEDDLKEKGFARMMRWSR
                     GVDLELYRPRDVRLFGDEPVFLYVGRISVEKNIKAFLDLELPGRKVVVGGGPQLNDLK
                     RQYPDVLFTGPKEGEALAEAYASADVFVFPSLTDTFGLVLLEALACGVPVAAYPVSGP
                     KDVLTDPACGVVGPDLQAAALQALDLDREAAREHALLYSWENSAQQFVDNVLSAHNLG
                     LPERRRLLPRLRGRRSARKAKKKKTAQVGGTRAASFSCVSLGDALGDLASTRRWLGA"
     gene            complement(12419..13288)
                     /locus_tag="AC16Y7_00100"
     CDS             complement(12419..13288)
                     /locus_tag="AC16Y7_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707814.1"
                     /translation="MPTRPIRGLAEQYAALEATLSGTPLGRWFLSEYMRRHRMPETQL
                     MLDAIARLERAMLKPKHQAGLTSVLGELVKISAAISRARAEIAQLRPADVDAELSNAT
                     EELDQIVETTEKATSEILSAAEDIQEVAWTLRENGVSPELGDRLDKHAIDIYTACSFQ
                     DITGQRVAKVVRTLRLIEQRINAVIDIWGAHDIAHKMSPPVEAGLLNGPQGEGAGLRQ
                     DDVDRALSQPEKGPDAAPAQAPEPAPEPHMRPEATRGEAPAGEARGGERFERPEPLVV
                     PQLHWVKRAALFG"
     gene            13406..14644
                     /locus_tag="AC16Y7_00105"
     CDS             13406..14644
                     /locus_tag="AC16Y7_00105"
                     /inference="COORDINATES: protein motif:HMM:NF037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5707815.1"
                     /translation="MANHATSSPSLAWRLGFLYAALFLVIGCYLPYMPVWLSWRTLSA
                     DQIAILLATPLYIRIVFTPVISFVADLLGNRRGIVIALAWGSLGSFALLWVSDGFWQM
                     MLAIALLAVNWTTIMPLVETIAISGIRKSGLDYGRVRLWGSLTFIGASLAAGIVIQFR
                     DAGTVLPMLMTATALLIFAAYLVPRDVEPKRQGSPVRRLRLRHAFALARAPLFLLFLL
                     AAAMIQGSHALLYAFGSLHWRAQGFSGGTIGALWSIGVIAEVALFAVSGRLVSYVGST
                     RLMVLAGGAAVVRWAVLALDPPLLATGLVQTLHALSFGATHLAAIHFLTHAVPEDRSA
                     TAQGLYAAVVAGLVLGSVTIACGPLYEAYGGQAYAAMVLLAGIGTAAAALLGRHWQGG
                     LVVGDQPHSAGKGVKTMPSR"
     gene            complement(14596..>15542)
                     /gene="dgcA"
                     /locus_tag="AC16Y7_00110"
     CDS             complement(14596..>15542)
                     /gene="dgcA"
                     /locus_tag="AC16Y7_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyl-D-Glu racemase DgcA"
                     /protein_id="HIR5707816.1"
                     /translation="WPIRGGFTIARGSKHEAHVVVAKIGDGRHTGRGECVPYARYGET
                     VEGVMADIEACALNHSLTRAALTKRLPAGAARNALDCALWDLEAKQSGTPAATAAGFS
                     TLQPVPTAFTISLGDPGTMAAEAKAASAHTLLKLKFGGAGDTARLQAVRAAVPTARLI
                     ADANEAWQPHETESLLAIAADAGVELIEQPLPAGNDAMLGEIERPIPVCADESVHDSA
                     SLPPLAGRYDAVNIKLDKTGGLTEALATAARARDLGLKIMVGSMVATSLAMAPALLLA
                     QDADWVDLDGPLLLERDRDAALTYRDGMVFTPLPALWG"
BASE COUNT         2685 a         4966 c         5013 g         2878 t
ORIGIN      
        1 gcccgcacta cttcaagccc accgagttcg tgattgccga aggattgttg ggctacacca
       61 cgcgcgccat gcgcgattgc tacgacgtga agatctatct cgacccggaa gacgatctcc
      121 ggatcaaatg gaaaatccat cgggacacca gcaagcgcgg ctatgggctt gaggaggtcg
      181 tcgcgtccct ggagcggcgc aagcaggaca gccccaactt catccatccc cagaggacgt
      241 tcgcggacat cgttatccgc ttccagtcgc caaaaggggc caatgggtcc gatacccaac
      301 tcgatgtgcg ccatctcctg cggcctactc tgccgcatcc ggacttcacg ccgcttttcg
      361 atgggtccag caacgccggg ctccatctcg agctggtgcg cgataaggat ggaaaacccg
      421 tcgacgtact tgagatcaac ggcaatatct ccgacaagag ggccgagcgg atcgaggatc
      481 tgctgtggaa tctcattccg gaggcgggcc acctgcgcga ccaggtgggc cgcatcgatg
      541 tcgcgaacgg cgcaaacaag agccacccgc tggcgctcac gcagcttctt gtgggctatc
      601 acgcggtcaa ggcggcgatg ggcgtccgcg cctactagaa aaaggacttt gggggacaat
      661 agatgagtga ggccctggca caggatcagg cattgtctgg agcgagccac gacgacatgg
      721 cgaacgtcat ccgcgcgctc gccatggacg cggtgcagaa agcggcctcg ggccatccag
      781 gcatgcccat gggcatggcg gacgtcgcca cggtcctgtt cacccgcttt ctgaaatacg
      841 acgccgccca cccggaatgg cccgaccgcg accgcttcgt gctctccgcc ggccacggct
      901 cgatgctgct ctacgcgctg ctctatctca ccggctatcc agacatggat atcgagcagc
      961 tcaagaattt ccgccagttc ggcgcgcgca ccgcggggca tcccgaatac ggccatgcgc
     1021 cggggattga gaccacgacg ggcccgctcg gccagggcat cggcaatgcg gtcggcatgg
     1081 cgctcgccga gcgcctgctc aaccagcgct acggcgacga catcgtcagc cactatacct
     1141 attgcatcgc tggcgacggc tgcctgatgg aaggcctcag ccaggaggcg atctccttcg
     1201 ccggccacct caagctcggg cggctgatcg tgttgttcga cgacaacgag atctccatcg
     1261 acggcggtac gaacctcact ctgtcggacg atcagctggc gcgcttccgg gcgtccaaat
     1321 ggcatgtcgc gacggtggac ggacacgatc cagaggccgt ggcgctcgcc atcgagaacg
     1381 cgcgcggcgt caccgaccag ccttcgatga ttgcctgccg cacggtgatc ggctatggcg
     1441 cgccgaacaa ggcgggcacg gccgccaccc atggcgcccc gctcggcgaa gacgagatcg
     1501 ccggtgcccg ccaggagctc ggctggccgc acgcgtcctt cgaggtgccc gatgcggtcc
     1561 tcgagagctg gcgcgcggca ggcatgtgtg gccgtgcgga ctacgacaag tggatggagg
     1621 ccgccggccg gctcgatgcc gcatcgcgcg cgcgcctgac cgatcccatc gacgcgaaag
     1681 tcggcgagga catcatccgc gccatggccg gcatcaagac ggacttcgcg gccgaaggcg
     1741 ccaagctcgc cacgcggcaa tcttcgcaga aagtgctgga gcggctgatg ccggtcgtgc
     1801 cggggctcat cggcggctcg gcggatctaa ccggttcggt gggcacgctc accaagcagc
     1861 acggcatcgt caagccggac gacttcgccg gcaactacat ccattacggc gttcgcgagc
     1921 atgccatggg cgcgagcatg aacggcctcg ccttgcatgg cggcatcgtg ccttacgcgg
     1981 gcacgttctt ggtgttctcc gattactgcc ggccgtcgat taggctgtcg gcactcatgg
     2041 agcagcgcgt cgtctatgtg atgacccacg gttccatcgg tcttggcgag gacggtccca
     2101 cgcatcagcc ggtcgagcag ctcgcagcgc tgagggccat gccccagctt cacgtgatgc
     2161 gcccggcgga ctccgtcgaa tgcgccgaat gctgggagct ggccttgctg tccaaggacc
     2221 ggccgacgat catgacgctg acccgccagg gcctgccgct gctgcgcacc acgcccacgg
     2281 acgacaatct ctcagccaaa ggcgcctatg tcctcatcga gccggaaggc ggccgggacg
     2341 tcaccgtgct ggccacgggc tcggaggttt cgttggcggt gaatgccgca aaaatgctcg
     2401 aagagcaggg catcaaggcc gccgtggtgt ccatgccctg ttgggagctg ttcgaggcgc
     2461 aagacgaagg ctaccaatcc gccgtcctcg gcaccacacc ccgggtgggc gtcgaggccg
     2521 cggtcggctt cggctgggac aaatggttgg gcgagaacgg aaagtttatt ggcatgcgcg
     2581 gattcggcgc gtctgccccg gcccaggagc tttataagca tttcgagatc accccggagg
     2641 ccgtcgtggc cgcggcccgt gatctgattt caaactagag gtaaggagca catggctaac
     2701 acgcgcgtcg ccatcaacgg atttggtcga attggcaggc tcaccttcag ggccttcctg
     2761 gaggccggcc gcaaggacct ggatttcgtg gcgatcaacg atctcggctc tgccgacatg
     2821 aatgcgctgc tgctcgagtt cgacagcgtg cacggcaggc tcccggccaa tgtttccgtc
     2881 tccgacggcc gcctcaaggc cgacgggcag gacgtcgccg tgctctccga agcggaccct
     2941 gagaagctcc cttggggcga actcggtgtc gacatcgtca tggaatgcac cggccgcttc
     3001 accaagcgcg agaccgctgc gcagcatctg gaagcaggcg ccaagcgcgt gctcgtctcc
     3061 gcgccgtgct ccggcgccga cctcaccgtg gtctacggcg tgaacgacga caagctcacc
     3121 gagaaggacc acgtggtctc caatgcctcg tgcacgacca actgcctggc tccggtggcc
     3181 gaggtgctgc acaaggcggt cggcatcgag cgcggctaca tgaccacgat ccacgcctat
     3241 accggcgacc agcgcaccgt ggacacgctc cacaaggacc cgcgccgggc ccgcgcgagc
     3301 gccgtgaacc tgatcccgac ctcgaccggt gcggcgaaag ctgtcgggct cgtgctgccc
     3361 gagctgcaag gcaagctcga cggcgcttcg atccgcgtgc cggtgcccaa tgtcagcctg
     3421 atcgatctga ccttccagtc ctcgcgcccg accacggtgg aggaaatcaa cgacgccatg
     3481 accaaggcgg cctctaaagc gccgctcaag ggcgtcatgg gcatcaacga gcagccgctc
     3541 gtgtcctcgg atttcaatca cgacccgctg agctcggtgt tcgacgctac ggggacgcaa
     3601 gtcatcgacg gcacgttctg ccgcgtggtg tcctggtacg acaacgagtg gggcttctcg
     3661 aaccggatga acgacaccgc ggtcgccatg ggacgactta tatgagcgat gacgccggca
     3721 tgggcggcgt cgacctgtcg cgcatcaaga ccatcgatgg tctcgacgta aagggaaagc
     3781 gcgtgctcat gcgtgcggac ctcaacgtgc cggtcgagaa cggccacgtc gccgacgcaa
     3841 cgcgcatcga gcgcgcactg ccgaccatag aggcgcttcg ctccggcggt gccaagctcg
     3901 tcatcatgtc tcactttggc cgtcccaaag gcgagcggtc gccggagaca tcactcaagc
     3961 ctgtcgccgc caagctgcaa gaaatgctcg gcacgcgcgt ttcgttcctg cacgattgcg
     4021 tggggccaga cgtcgaggcg ggcgtcaatg cgctgaaaga cggcgacgtg gcgctgctgg
     4081 agaacctccg ctaccacaag ggcgagacgg ccaacgatgc cggcttcgcc aagcagcttg
     4141 cggcgctagg cgatatctat gtggacgatg cattttcctg cgcccatcgc gcgcatgctt
     4201 ccgtggaagc gattacgcat ctgctgccgt cctatgccgg caaatcgatg atggcggaga
     4261 tcgaggcgct ggccagcgcg cttcacaacc cgaagcggcc ggtcatggcc attgtcggcg
     4321 gcgccaaggt ctccacaaag atcgacgtgc tcaccaacct cgtgcagcgc atgaacgtgg
     4381 tggtcgtcgg cggcggcatg gcgaatacct tcctctacgc ccaaggcgtg gatatcggca
     4441 attcgctgtg cgagcccgac ctcaaggaca tcgcgctcga tattatcgac aaggcgaagg
     4501 tgtctggctg cgagatcatg ttgcccgtcg accaagtcgt cgccaaggag ttggcgccgg
     4561 gtgccgagac cgtagtgtgt gacaccggca ccgttccggc cggcatgatg atcctcgacg
     4621 tggggccgaa gagcatcgcg cggctgaagg aaaagttagg cgagatggcg actctgttat
     4681 ggaacgggcc gctcggcgct ttcgaaatca cgccgttcgg ggaaggcacg ttcgcgctgg
     4741 cgcgcgaggc aggccgtctc tctaaagacg gcaagcttat tacgattgcc ggcggcggcg
     4801 acacggtgcg cgccttgaac acgacgggcg cggcgcccga cttcacttat atctcaacgg
     4861 cgggcggcgc ctttctcgaa tggctggccg gcgagcagct gccgggcgtg gtcgcgctgg
     4921 ccggtggcga tacgaaacat tccgcggttg cgtgaggcac gaagcatgtc cgacgacatc
     4981 atcatcgcac cgtctatcct ctcggctgac ttcgccaagc tcggcgagga agtgaaggcc
     5041 atcgacaagg ccggttgcga ttggatccat atcgatgtca tggacgggca ctttgtgccc
     5101 aacatcacca tcggtcccga cgtggtgaag gcgcttcgcc cgcattgcga caaggtgttc
     5161 gacacccatc tcatgattgc gccgtgcgat ccgtatctcg acgcattcgc cgaggccggt
     5221 tccgacatca tcaccgtgca tgccgaagcc ggtccgcatc tggaccgctc gctgcagcac
     5281 atcaggagcc tcggcaagaa ggcaggcgtc agcctcaccc cgtcgacccc tgagacggcg
     5341 atcgaatacg tgctcgataa agtcgatctg attttggtga tgacggtgaa cccgggtttt
     5401 ggcggtcagt ccttcatccc tgagcagctg aagaagatca gcgcgatccg taagatgatc
     5461 ggcgatcgtc cgattcacct ggaggtggat ggcggcgtga acccggagac ggcggggctt
     5521 gtcgccgagg ctggcgccga tgttctcgtg gccggctctg ccgtgttcaa gggcggtgct
     5581 tacgcgaaga acatcgcggc gatccgcgat gcggcgttac gtgcccgcga tgccgcttga
     5641 gggcacggtt aaccggaatt ttagtgaccc cggtcactga ctgtggcgct gacctgtgat
     5701 caatcctctt cacgacttgg tgccaggcct tgctcttccg atgcgggcgc aatagctaga
     5761 aacccatgga tgtcacctat ttcggcgctc tgttcgccgg cttcttgagt ttcctcagcc
     5821 cctgcgtgct gccgctcgtg ccgccttatc tgtgcttcct cggcggcacc accttcgatc
     5881 agctggccgg cgaggacgag acgcctcccc atgtctacac cacggtggtg ttgtcctctg
     5941 tcgccttcgt gctcggtttc accaccgtgt ttgtcatctt gggtgccacg gcgacggtgt
     6001 tgggccagct cgtgctggag aacctgccga tccttgccaa ggtcgcgggc gtcatcatca
     6061 tcatcgccgg cctgcacttc ctcggcgtca tccacattcc gatcctccat cgcgaggcgc
     6121 gctatcatgc cgacacgcgg ccggcggggt tggtcggcgc ctatatcatc ggcttggcgt
     6181 tcgccttcgg ctggacgccc tgcatcgggc ccgtgctcgg ggctattctg gcggtcgccg
     6241 ccggcgagga ctccgtgcgc cagggcgtga gcctgctctt cgtctattcg ttggggctcg
     6301 gcattccgtt cattcttgcc gccgtcgccg ttcggccgtt catgaatgcc atgggccgct
     6361 tcaagggcca catgggcacg atcgagaagg tgctgggcgc gcttctggtc ctcaccggca
     6421 ttctgttcct cacgaactcg atgacgctga tcgcgggctg gatcctgcgc acgttcccgg
     6481 cgcttgccac gattggctga cgaggctcag tccgattgcc tctggaggct tgcctctaga
     6541 ggcttgctct tgggcgccag cggtgaatag tggcgcctca tgacccctag atcgacgtcc
     6601 atcgcgctca ccatcgccgg ctccgattct tccggaagcg ccggcatcca agccgacctc
     6661 aagacgttcg gtgcgcttgg cgtgcatggc gcttgcgtcg tcgccgcggt caccgcgcag
     6721 aacacgcaaa aggtcgaggc catcttgcca gtgccgccgg agttcgtact ggcgcagatg
     6781 caggccgtgg caaacgacat cgatgtcggc gccatcaaga ttggcatgct ggcaacgcgc
     6841 gacaatatcg aggccgtcgc cgaggggttg acgctgttcg acgggacgcc ggtggtcctc
     6901 gatcccgtga tggcgtcgca tcggggcgac ccgctactcg atgagagcgc cgtggacacg
     6961 ctcaagacca tgctgatgcc gtgcgccacg ctggtgacgc ccaacctgtt cgaagcggcc
     7021 ttgctgacca gcgaggagaa ggcccgcagc gtggacgcca tggccgaaca agcgtggatg
     7081 ctgcgcggcc tcggcgccaa agccgtgctc gtcaaaggcg ggcatctcga cggcgaagcc
     7141 atcgacgtgc tgttgagcaa tgacggcgta gaacgcctcg aagcgccccg gcatgagacc
     7201 gggaatacgc acggcacggg ctgcaccctg tcctctgcca tcgccgcggg gctcgcgcat
     7261 ggcaagccgc tggagaaggc ggtgaaggac gcgaaggtct atgtcaccgc ggcgatcatc
     7321 gccgccgacg agatcgcagt ggggaagggc cgggggccac ttcaccactt ccatgcactg
     7381 tggccgtagc cgtaaagggc gctagccgtc ggcgacgact ttgagcttct gttcgccgag
     7441 gccttggacg tggagcacca tctcgtcgcc aggcttaata aagacgcgag gtttgcgtgc
     7501 catgccgacg ccgggcggcg tgccggtggt gatcacgtcg ccgggcatca gcgtcatata
     7561 ggtgctgata tagctgacga ggtcggcgac ggtgaaaatc atggtctcgg tattgcccgt
     7621 ctgcatgcgc tccccgttga ggtccatggt gagatcgagc gcttgcggat ccgtaacctc
     7681 gtccgtggtg acgagccaag ggcctaaggg tccgaagctg tcatagctct tggctttcat
     7741 ccattcgccg ggtccgcctt tttgcagact gcgctcggag acatcgttgc agatgcaata
     7801 gccggccacg tagtcaagcg cgtcgtcttt cgagacgcgc ttggcgggcg tgccgatgac
     7861 caccgcgagc tccacctcgt agtccatgcg ctggaagtcc ggcggcgcca gcaccggatc
     7921 ggtgggcccg gagacgcagg agttcgcctt gcaaaacacg atggggactt cgggaatgtc
     7981 catgccggtc tcttcggcgt ggtcgcgata gttgaggccg atgccaagaa atttcggcac
     8041 gacgccgatg ggcggaccaa gcctggggtt cccctcgacc agcggcagcg tttccggatc
     8101 gatcgccttg agacggtcaa ggctcgcgcg gccaagcgca tccccggcaa gatcgccgat
     8161 ctccccggac aggtcgcgga tcagcccctt ggcatctaga acgcccggct tttccgcccc
     8221 gggtgcgcca aaacgtacaa gcttcattgt cttcccccag ccatgatttt ctgcatgatg
     8281 cacgcgtggc gatggggcgg caacggcctg ccggcttgtc tcccgcgcaa cgccaatccc
     8341 gaaagacgaa aggtgccggt tccgatgaga cttcgtttgc gttcgcctcg cctcgggacc
     8401 ttgattgccg tctttctcgc aaacgccctg actgtcctat ccgcgctgcc ggcgtccgcc
     8461 aacccgtatc agcagttgga tggcgcttgg accgggagcg ggacagtgac cccgttgaaa
     8521 ggggcgccag agaaggtcag ctgcaacacc acctataaga ctgaagggtc cgtggtacgg
     8581 cagaccgtgc gctgcgcgag cgcgagccac gctttcgccg ggaatctgga actgacctat
     8641 gagggcggcc ggatccgcgg cgcctggcgt gagcaggtcc atggcgcctc cggcgacgtc
     8701 agcggcacgg ccaacggcag ctccatccgc gcgcgcctca gcggccagac cttctcaggc
     8761 cgcatgcgca tcgatgtcga tgggcccaaa cacacgatcg agatcgtcca gcgcgacacc
     8821 ggatcgggcg cttatcgtcc cgtcgcctcg atctcgttgc accgctagag tcacctgtgg
     8881 cggctgcgcc aaggccgttg ctgaattgcc acaaagcctc acaaagtttc ccccaaacgg
     8941 gtgcagccac ggccccccgc gagggtgcag cgatgcgcgt tgcagcccgc ctggggcgcc
     9001 tcggaagcaa cataatatgt tgaaatacaa taataaaacc aggtgacgcg ccgcgccggt
     9061 caagccgctt tgcatttccg caacagtgcg gcgacgattt gcgtttcgcc cctcgcgcat
     9121 tgttgaagcg cgcgccgggg ctcgctagct tttcatcaca acacggggga aggggttggg
     9181 ggctttcttc tcaccggaac gaaagcaaga gcaaggttgc cgacgagccg aagcaagaac
     9241 caagaccaag aaagacggcg cagaaaaaat gggggggcga gtcgaagccc gctgtaaagg
     9301 tttaggcctt tcgcgttcgg ctcgtttcgc agggaagacc ggcccggctt tgtccgggcc
     9361 ggttcttttt tggcaccagg tcaaagtccg agcttcgtca gcgtcgtctc aacgcgcccc
     9421 aaatagctcc ccccgaacag ccgcacatgc acgagcgtgg gatagagatt gtagagatcc
     9481 cgccgcagct cgtggaaccc cggctccagc ggcgacatcc cctcataggc ctcgaagaac
     9541 gcatcgccga acgtcccgaa catcgtcgtg aaggcgagct cgatctccgg atggccgcaa
     9601 tagatcgccg gatcgacgaa cccggcgatg cggtttccgc acacgagcac gttcccggtc
     9661 catagatcgc cgtgcagcag actggggaag ggcggctcgg tcagaaagtc ctcgagcttt
     9721 gatgcgagcg tttcgatgcg gccatagagc gcggccggca gccggccttc gtcctttgct
     9781 gcgcgcgcca tatagaggag ccgctcgtcg cggaagaacg ggatccagca ttggctttgc
     9841 gggttgggct ggtgcagggg gccgatcagc gtgtcccgcg catagccgaa gccgtcccgc
     9901 ggcgtggcat ggagtgcggc aatgagcctc gcggcatcgc gctctacgtt ggcggtgatc
     9961 gtcccgccgt ccgtctcgat gaagtccatg acaaggaggt cgggctcggc cgcatgaacc
    10021 tgcggaacgg gcaagtccga cgtccgcgaa agctcgccga gcatatagcc ttcgagcgca
    10081 agcgacggcc cggagcgcgc gttgtccttc gccttcaccg ccacatgccg gccgtcttgg
    10141 agccgcgcgg acaggccggt cagaccgaat cccactggca atggcgcggt gtcgacgacg
    10201 cccgagccga gcgccccctc aagccgtata cgcaaatcgc cgttggtcat cgccgtttac
    10261 caagtcctta agcagtcgcc agtgtcacat ccctgatact aaatcacctt agatcgcggt
    10321 ttatcgtgct gaacactcga atagacgatt acacgcagcc agacgccgag cagaggcgtt
    10381 atcgcgcgct gtttatttcc gacattcatc ttggcaccaa ggcctgtcag gccgaagcct
    10441 tcctagactt cttgcgctac cacgacgcgc cccggttcta cctggtcggc gatatcgtcg
    10501 atttctggcg catccggcgc ggggtctact ggccgcaagc ccacaacgat gtcttgcaga
    10561 agctgctccg caaggtccgc aaagggaccg agctcgttta catccccggg aatcacgacg
    10621 aggtcatgcg ggagtattgc ggctcgcaat tcggcggcat cgccattgag cgccagacga
    10681 tccatacggg cgcggacgga cggcgctatc tgatcatgca tggcgacgag ttcgacgtgg
    10741 tcgtgcgcta cgccaagtgg ctcgccttct tgggcgactg gagctacacg gcggcgcttt
    10801 ggttcaacac gcacctcaat gccgtgcgcc gctggttcgg catgccgtat tggtcgctct
    10861 cggcctatct gaagcacaag gtcaagcggg cggtgaatta catcggcgag ttcgagcaag
    10921 cgttgtcgca agaggcccgc cgcaatggcg cccaaggcgt gctctgcggg cacatccatc
    10981 acgccgccat gcgtcaggtc ggcgacgtcg tctacatcaa taccggtgac tgggtcgaaa
    11041 gctgtacggc ggtgggcgag acggaggagg gtgtcttcga gatcatccac tggaatccgc
    11101 acaccggcgt tcggacgtcg ggaacgccgg ccaaggagct tgaggccgca gcttgatgcg
    11161 catcctcgtt gccaccgacg cctggtttcc caaggtgaat ggagttgtcc gcacctatga
    11221 gcggcttgcg caagagcttc cgaagcttgg cgccgatatc agctttctca cacccgcccc
    11281 gttctacacg ctgccttgcc ccacctatcc ggagatcagg ctatcgctgg tcagcccaag
    11341 catggtggcg cgccatatcg aagaggcgcg gccggacttc atccatatcg ccacggaggg
    11401 gccgataggg ctgatggcca ggggctactg ccgcaagatt aagcgcccct tcaccacgag
    11461 ctaccacaca cgctttcctg aatatgtttc ggcgcgcctg ccgatccccg agtcttggtg
    11521 ctacgccttc cagcgccagt tccataacag cggcgcgggg ctgttcgtgg cgacccagtc
    11581 ggtcgaagac gatctcaagg aaaagggttt cgcgcgcatg atgcgctggt cccgtggcgt
    11641 cgacctggaa ctctaccggc cgcgcgacgt gcggctcttc ggcgatgagc ccgtgttcct
    11701 ctatgtgggc cgcatttcgg tggagaagaa catcaaggcc tttctcgatc tggagcttcc
    11761 ggggcggaag gtcgtggtcg gcggcggccc ccagttgaac gatctcaagc gtcaatatcc
    11821 cgacgtgcta ttcaccggtc caaaggaggg cgaggcgctg gcggaagcct atgcctcggc
    11881 ggatgtgttc gtgttcccaa gcctgaccga cacgttcggt ctggtgctgc tggaggcctt
    11941 ggcgtgcggt gtacccgtcg cggcctatcc cgtcagcggg cccaaggacg tgctgacaga
    12001 ccccgcttgc ggggtcgtgg ggcctgacct ccaggcggcg gcgcttcagg cgctggacct
    12061 cgaccgcgag gcggcacgcg agcatgcgct gctctatagc tgggaaaact cggcgcagca
    12121 gttcgtggac aatgtgctgt cggcccacaa tctcggcctg ccggaacggc ggcggctcct
    12181 gccgcgcttg cgcggacggc gaagcgcgcg gaaggcaaaa aagaaaaaaa cggcccaggt
    12241 ggggggaacc cgggccgctt cattctcatg cgtcagtttg ggggacgctt tgggggatct
    12301 tgcgtctacc cggagatggt taggggcctg aatcttttca aggtgagggt gccgactttt
    12361 tgcccgccgg acgctaacct actgattcaa tagcaaaaaa atcgacgaaa aagtgtggct
    12421 agccgaacag ggcggcccgc ttgacccagt gaagctgggg cacgaccagc ggctccggtc
    12481 gctcgaagcg ctcgccgcca cgagcttcgc cagcaggggc ttcgccgcga gtggcttcgg
    12541 gccgcatgtg aggctccgga gctggctctg gggcctgcgc cggggcggcg tccgggccct
    12601 tttccggttg gctcagggcc cgatccacgt cgtcctgccg cagcccggcc ccctcgcctt
    12661 gaggaccgtt cagcaggccg gcctccacag gcgggctcat cttgtgggca atgtcatgcg
    12721 cgccccagat atcgatcacg gcgttgatgc gctgttcgat gagacgcaaa gtgcgcacga
    12781 ctttggccac gcgctgtccc gtgatgtctt ggaacgagca agcggtgtag atgtcgatcg
    12841 cgtgcttgtc gagcctgtcg ccgagctccg gcgagacgcc attctcgcgc agcgtccagg
    12901 cgacttcctg gatgtcctcg gctgcggaca aaatctcaga ggttgccttc tcggtggtct
    12961 cgacgatctg atcgagctct tccgtcgcgt ttgagagttc cgcgtcgacg tcggccggac
    13021 ggagctgcgc gatctcagcc cgtgcacgcg aaatcgcggc gctgatcttc acgagctcgc
    13081 cgagtacgct tgtcagccct gcctgatgct tcggcttgag catggccctt tcaagccggg
    13141 cgatggcgtc gagcatgagt tgcgtctcgg gcatgcggtg ccggcgcatg tactccgata
    13201 agaaccagcg tccgagcggc gttccgctca atgttgcctc aagagcagcg tattgctccg
    13261 ccaggccacg tatgggccgc gttggcattc gccgcgatgt atcggcgcct tttggagctc
    13321 gttccggcat ggtgatggcg aagctatctg attcggagta aggcccgaac gtagcaaccg
    13381 cgccgcaagg gagtcgttaa cgtctttggc gaaccacgcc acatcgtcgc cctcactcgc
    13441 atggcggctc ggctttctct atgccgcgct attcctggtt atcggctgct acctgcctta
    13501 catgccggtg tggctaagct ggcggacgct gagtgcggac cagatcgcca ttcttttggc
    13561 gacgccgctc tatatccgca tcgtgtttac ccccgtcatc agctttgtgg cggacctgct
    13621 cggcaaccgg cgcggaattg taattgcgct cgcctggggg tcgctgggct cgttcgccct
    13681 gctttgggtg tccgacggat tttggcagat gatgctcgcc atcgcgctgc tggccgtcaa
    13741 ctggacgacg atcatgccgc tcgtcgagac gatcgcgatc agcggtatcc gcaagagcgg
    13801 gctcgactat gggcgcgtgc ggttgtgggg ctcgttgacc ttcatcggcg cgagccttgc
    13861 cgcgggcatc gtcattcagt ttcgggatgc gggcaccgtg ctgccgatgc tcatgaccgc
    13921 gacggcgctt ctgatcttcg cggcgtatct ggttccgcgg gacgtcgagc cgaagcgtca
    13981 aggcagcccc gtgcgccgtt tgcgcctgag acatgccttc gcgctcgcgc gcgcgccgct
    14041 ctttctgctg tttctacttg ccgcggccat gatccaaggc agccatgcgc tgctttatgc
    14101 tttcggctcg ctccattggc gcgcgcaagg cttctccggc ggcacgatcg gcgcgttgtg
    14161 gtcgatcggc gtcatcgccg aagtggcgct gttcgcggtc tcaggcaggc tcgtgtccta
    14221 tgtcggcagc acgcggctca tggtgctggc tggcggcgcg gctgtcgtgc gctgggccgt
    14281 cttggccctc gatccgccgc ttctagccac ggggctggtc cagaccctgc acgccttgag
    14341 cttcggcgcc acccatctcg ccgccatcca tttcttgacc catgcggtgc cggaggatcg
    14401 ttccgccacg gcgcaaggcc tttacgcggc cgtggttgcc gggctcgtct tgggctcggt
    14461 gacgatcgcc tgcggtccgc tttacgaggc gtatggcggc caggcctatg cggccatggt
    14521 gctgcttgcg gggatcggga cggccgcggc ggccttgctc ggccgccact ggcagggcgg
    14581 gcttgtcgtg ggcgatcagc cccacagcgc aggcaagggc gtgaaaacca tgccgtcgcg
    14641 gtaggtgagg gcggcatccc ggtcgcgctc caagagcaag gggccgtcta ggtccaccca
    14701 gtcggcgtct tgggcgagga gcagggcggg cgccatggcc agcgacgttg cgaccatgct
    14761 gcccaccatg atcttgagac cgaggtcgcg tgcgcgggcg gccgtggcca acgcttcggt
    14821 gagaccgccg gtcttgtcga gtttgatgtt caccgcgtca tagcggccgg ccagcggcgg
    14881 aagcgatgcg ctgtcatgca cggactcatc ggcgcaaacc gggatcggcc gttcgatctc
    14941 ccccagcatc gcgtcgttgc cggccggcag cggctgctcg atcagttcca cacccgcatc
    15001 cgccgctatg gccagaagcg actccgtttc atggggttgc caggcctcgt tggcatcggc
    15061 gatgagacgt gcggtgggga cggccgcgcg cacggcctga agacgcgccg tatcgcccgc
    15121 gccgccgaac ttcagtttga gcagcgtatg cgcggacgcg gcctttgcct ccgccgccat
    15181 ggtgccggga tcgccaagtg agatggtgaa ggccgtggga acgggctgaa gggtcgaaaa
    15241 acctgctgcc gtggcggcag gcgttccgga ctgcttcgcc tccaagtccc atagggcgca
    15301 gtcgagggca ttccgcgcgg cgcccgccgg caggcgcttg gtaagcgcgg cgcgcgtgag
    15361 gctatggttg agcgcgcagg cctcgatgtc ggccatcacg ccttccacgg tctcgccata
    15421 gcgggcatat ggcacgcact cgccacgtcc ggtgtggcgg ccatcgccga tcttggccac
    15481 gaccacgtgc gcttcatgct tggacccgcg ggcgatggtg aagccgcccc ggatcggcca
    15541 gc
//
LOCUS       DBNHVU010000003         3242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_75764, whole genome shotgun sequence.
ACCESSION   DBNHVU010000003 DBNHVU010000000
VERSION     DBNHVU010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_75764"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="AC16Y7_00115"
     CDS             <1..215
                     /locus_tag="AC16Y7_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR5707817.1"
                     /translation="RVGTEDNDSVLQTYYGHSFIADHTGDMVAELGEGAEGVIIAEFD
                     VDDIAAYRADWGFFRDRRTDLYGKSI"
     gene            212..1129
                     /locus_tag="AC16Y7_00120"
     CDS             212..1129
                     /locus_tag="AC16Y7_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR5707818.1"
                     /translation="MTAVLNPLDDPTTTRIVAGDTEAVFVPGHGMLGISLRHKGLEYL
                     RRIDDLKTYAAMNQTAGIPFLFPWANRLGGFQYEIEDRGVTLSGHSPTLRYDGNGLPM
                     HGLMWPHLAWSIETTAPDSLTAQYDWSHPKGLVIFPYPCRLTMTATVAPGNLTIETAL
                     APTGDIGVPVSFGFHPYLGLPDVPRQDWQVTLPEMRHLTLDKRMIPTGGSEPFPGLDA
                     ALGDRAFDDGFALLGDSATLSVAAGGNRISVDLLEGYPYTQVYAPHDKDFLAFEPMTA
                     PTNALISGEGLRLVPPGETFSAKFRFKVD"
     gene            1204..2373
                     /gene="lpxB"
                     /locus_tag="AC16Y7_00125"
     CDS             1204..2373
                     /gene="lpxB"
                     /locus_tag="AC16Y7_00125"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HIR5707819.1"
                     /translation="MSRQEPLIFIIAGEASGDNLAGRLMYALKQQTEGRVRFAGVGGP
                     QSEAQGLKSLFPMQELSLVGLAEVLPHLPLLAKRLSQTTAAARELKPDVMVTVDAPSF
                     GLRISGRLRGSGIPVVHYVAPQAWAWRAGRAKTIGKRMDHLMALLPFEVPFFADHGIP
                     CTYVGHPAIESAAAGDGKVFRRKHNLSDDALVLCVVPGSRAGEVRRILPIFTEAISRL
                     KDRHPDLHVVIPVAPAVAHQVETQTADWPLPVVRVTDPDERFDAFAACDAAMTKSGTV
                     TLELALADVPMVVAYKVNALTAFIVRRMGVNVEHASLVNLLANSEVVPELLQEGCTPE
                     TVANAVEDLLGSKEARETQRAGFRDVWKALGEATPPPSARAAKLVLDMIGRKQDA"
     gene            complement(2370..>3242)
                     /locus_tag="AC16Y7_00130"
     CDS             complement(2370..>3242)
                     /locus_tag="AC16Y7_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5707820.1"
                     /translation="QVAEEFGAEAFTDHQQIFDRVDAVSVAVPTPLHFDVSRELIEAG
                     KHVLIEKPITDTVESANIITKLAEERGTVLQVGHIERYSAAYRVISENIGRPLYLESY
                     RIAPWKKRGVEVDVILDLMIHDIDMIIGLVGAPVARVDAVGTGVMSHKADIANARISF
                     ESGCVANVTASRVSYKTERRLRAFAHNKYLNCDLGERKIFGYSLRGNPMTQGLSAIVT
                     EKIDIPQEDSLGNEVASFLDCIKTGKKPFVDGYAGSEALRVAAMINESIDEQLKKVQG
                     WATQDGAEVSPAEV"
BASE COUNT          596 a         1078 c          948 g          620 t
ORIGIN      
        1 accgcgtcgg cacggaagac aatgacagcg tcctccagac ctattacggc cactccttca
       61 tcgccgatca cacgggcgat atggtcgccg agcttggcga gggcgccgaa ggtgtcatta
      121 ttgccgaatt cgatgttgac gacatcgccg cctaccgcgc cgattggggc ttcttccgcg
      181 accgccgcac cgatctttat gggaaatcga tatgacagcc gttctgaatc ctctcgacga
      241 tccaaccacc acgcgcattg tcgccggcga cacggaggcc gtattcgtgc ccggccacgg
      301 catgctcggc atttcgctgc gccataaggg cctcgagtac ttgcgccgta tcgacgacct
      361 caagacctac gccgcgatga accagaccgc gggcattccc ttcctctttc cctgggccaa
      421 ccggcttggc ggattccaat acgagatcga ggatcgcggc gtgacgctca gcggccattc
      481 gccgacgctc cgctacgatg gcaatggcct gcccatgcac ggcctgatgt ggccgcacct
      541 cgcttggagc atcgagacga cggcgccgga cagtctgacg gcgcaatacg attggtctca
      601 tcccaagggc ctcgtcatct tcccctaccc ctgccggctg acaatgacgg caaccgtggc
      661 gcccggaaac ctcaccatcg agacagcgct cgcgccgaca ggcgacattg gcgtgcctgt
      721 cagcttcggt tttcatcctt atcttggact accggacgtg ccccgccagg actggcaggt
      781 gacgctccct gagatgcggc atctcaccct cgataagcgg atgatcccga ccggcggatc
      841 ggagcccttc ccgggcctcg atgcggcact cggcgaccgg gctttcgacg acggcttcgc
      901 gcttctcgga gacagcgcca ccctgtcggt cgcggccgga ggaaaccgca tctcggtgga
      961 cttgctggag ggctatccct acacgcaggt ctatgccccg cacgataagg acttcctcgc
     1021 cttcgagccc atgaccgcgc cgaccaacgc gctgatcagc ggcgagggct tgcggctggt
     1081 ccccccgggc gagacgttta gcgccaagtt ccgcttcaaa gtggactaga ggcgaatttt
     1141 cctggcgcag accccgtccc ttggcctaag tcctgcctcg atgagaccca gaaggaacga
     1201 cagatgagcc ggcaggagcc gctaatcttt atcatcgccg gcgaggcctc aggcgataac
     1261 ctcgccggtc ggctgatgta cgcgttgaaa caacagacgg aaggccgcgt gcggtttgcc
     1321 ggcgtcggcg ggccgcaaag cgaagcgcaa ggcctgaaaa gcctgttccc catgcaagag
     1381 ctgtccctcg tggggctggc cgaggtgctg ccgcacctcc cgcttctcgc caaacgcctc
     1441 agtcaaacga ctgccgccgc ccgcgaactg aagcccgacg tcatggtcac ggtcgacgcc
     1501 ccgagcttcg gcctgaggat ttcgggcagg ctccgcggct ccggcatccc ggtggttcat
     1561 tacgtggcgc cgcaagcctg ggcctggcgc gcgggccgcg caaagacaat cggcaagcgg
     1621 atggaccacc tcatggcgct cctgccgttc gaggtcccgt tctttgccga ccacggtatt
     1681 ccctgcacct atgtcggcca cccggcaatc gagtcggccg ccgccggtga cggcaaagtc
     1741 tttcgacgga aacacaacct ctccgacgac gcgctggtgc tctgcgtcgt accgggcagc
     1801 cgtgcgggcg aagtgcggcg catcctcccg atctttaccg aggcgatttc tcgtctgaag
     1861 gataggcatc ccgatctgca tgtagtcatt cccgtcgccc cggcagttgc ccaccaagtc
     1921 gaaacccaga ccgcggactg gccgttgccg gtcgtgcgcg tgacagatcc agatgagcgg
     1981 ttcgatgcct tcgctgcatg cgacgccgcc atgaccaagt cgggcaccgt gacactggag
     2041 ctcgccttgg cggacgttcc aatggtcgtc gcctataagg tcaatgcgct tacagctttt
     2101 atcgtccgcc gcatgggcgt caacgttgag cacgcctcgc tggtcaacct tcttgccaac
     2161 agcgaagtcg ttcccgaact cctgcaagag ggctgcacgc cagagactgt ggccaacgcc
     2221 gttgaagatt tgctgggctc aaaggaggcg cgggaaacgc agcgcgccgg cttccgcgac
     2281 gtttggaaag cgctcggcga agcgacgccg ccgccaagcg cacgcgcggc aaaactcgta
     2341 ctcgatatga tcggcagaaa acaagacgcc tagacttcgg cggggctgac ctccgcgccg
     2401 tcttgggtcg cccacccttg cactttcttc aactgctcat cgatgctctc gttgatcatc
     2461 gccgcgacac gcaacgcttc ggaccccgca tagccgtcga cgaacggctt tttgccggtc
     2521 ttgatacagt cgagaaacga tgcaacttcg ttgccgaggc tgtcttcttg cggaatatcg
     2581 atcttctcgg tgacgatggc cgacagccct tgtgtcatcg ggttgccacg caggctgtag
     2641 ccaaatatct tccgctcgcc gagatcgcag ttgaggtact tgttgtgcgc gaaggcgcgc
     2701 aagcgccgtt ccgtcttgta gctcacccgg cttgcggtga cattggccac gcagccagac
     2761 tcgaaactga tccgcgcatt ggcgatatcg gccttgtggc tcatcacgcc cgtgccgacg
     2821 gcatcgaccc ttgcgaccgg cgcaccgacg agcccaatga tcatatcgat gtcgtgaatc
     2881 atcaggtcga ggatcacgtc gacttcgacg ccgcgtttct tccacggcgc aatacgatag
     2941 ctctccagat agagcggcct gccgatattc tccgaaatga cacgataggc cgcggagtag
     3001 cgctcgatgt gaccgacctg aagcaccgtg ccgcgctctt ctgccagctt ggtgatgata
     3061 ttcgcgctct cgactgtgtc ggtgatcggc ttttcgatca gcacgtgttt gcccgcttcg
     3121 atcaattcgc gcgacacgtc gaaatgcagc ggcgtaggca cggcaacgct caccgcgtcc
     3181 acacggtcga aaatctgctg gtgatccgtg aaggcctcgg cgccgaactc ctcggcaacc
     3241 tg
//
LOCUS       DBNHVU010000004         8118 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1562559, whole genome shotgun sequence.
ACCESSION   DBNHVU010000004 DBNHVU010000000
VERSION     DBNHVU010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8118)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8118)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8118
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1562559"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="AC16Y7_00135"
     CDS             complement(<1..186)
                     /locus_tag="AC16Y7_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR5707821.1"
                     /translation="MADLQKIADDLSGLTVLEAAELAKILEEKWGVSAAAPVAVAAAA
                     GGGGDAAAAEEKTEFTVV"
     gene            complement(232..747)
                     /gene="rplJ"
                     /locus_tag="AC16Y7_00140"
     CDS             complement(232..747)
                     /gene="rplJ"
                     /locus_tag="AC16Y7_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR5707822.1"
                     /translation="MERAEKREVVSALHDAFAETGVVVVAHYAGLSVADMTKLRSDMR
                     EAGGRVKVAKNRLVKLALEGTDAKGITDLMKGPTCLAFSDDPVTAPKVAVKFAKGNEN
                     FVILGGTMGTTLLDTSAVNSLAELPSLDELRGKLVGLLQAPAGKIARTVGAPAAQLAR
                     VFGAYGNKDAA"
     gene            complement(1085..1783)
                     /gene="rplA"
                     /locus_tag="AC16Y7_00145"
     CDS             complement(1085..1783)
                     /gene="rplA"
                     /locus_tag="AC16Y7_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR5707823.1"
                     /translation="MSKPGKRFRTAADAIEAEKLYGIEDAVKLIKSGAKTKFDETIEI
                     AMNLGVDPRHSDQMVRGVCQLPHGSGRKLRVGVFAKGDKAEEAKKAGADVVGAEDLVE
                     QVQKGVIEFDRCIATPDMMPLVGRLGKVLGPRGLMPNPKVGTVTADVAAAVGAAKGGA
                     VEFRVEKAGVIHAGVGKASFTEEALVENIRAFTDAVNKAKPSGAKGTFVKRIAVTSTM
                     GPGVKVEPASLGSA"
     gene            complement(1787..2215)
                     /gene="rplK"
                     /locus_tag="AC16Y7_00150"
     CDS             complement(1787..2215)
                     /gene="rplK"
                     /locus_tag="AC16Y7_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR5707824.1"
                     /translation="MAKKIDGYIKLQVPAGQANPSPPIGPALGQRGVNIPEFCKAFNA
                     ATQKLEPGSPIPTLITVYADRSFTFEMKTPPASYLLKKAAKIGKGSKEPGRAGAGKVS
                     QDQLREIAETKMVDLNANTVEQAMKIIAGSARSMGLEVQE"
     gene            complement(2264..2794)
                     /gene="nusG"
                     /locus_tag="AC16Y7_00155"
     CDS             complement(2264..2794)
                     /gene="nusG"
                     /locus_tag="AC16Y7_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR5707825.1"
                     /translation="MAMRWYIVHAYSNFERKVAESIKERAAAAGFGDKFEQVLVPMEE
                     VVEMRRGRKVASERKFFPGYVLVKMELDDETYHLIKDTPKVTGFLGTENKPMPITEEE
                     AGRILHQVQEGVERPKPSVTFEIGEQVRVADGPFASFNGLVEEVDEERARLKVAVSIF
                     GRATPVELEYAQVEKL"
     gene            complement(2799..2996)
                     /gene="secE"
                     /locus_tag="AC16Y7_00160"
     CDS             complement(2799..2996)
                     /gene="secE"
                     /locus_tag="AC16Y7_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HIR5707826.1"
                     /translation="MAKTNPFVFLQQVRTEVSKVTWPTRRETVITTIMVVIMSILAAL
                     FFLGVDWVLGTVVRTILGMRF"
     gene            complement(3086..3162)
                     /locus_tag="AC16Y7_00165"
     tRNA            complement(3086..3162)
                     /locus_tag="AC16Y7_00165"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3126..3128),aa:Trp,seq:cca)
     gene            complement(3225..3298)
                     /locus_tag="AC16Y7_00170"
     tRNA            complement(3225..3298)
                     /locus_tag="AC16Y7_00170"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3264..3266),aa:Gly,seq:tcc)
     gene            complement(3324..3408)
                     /locus_tag="AC16Y7_00175"
     tRNA            complement(3324..3408)
                     /locus_tag="AC16Y7_00175"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3372..3374),aa:Tyr,seq:gta)
     gene            3611..4408
                     /gene="rlmB"
                     /locus_tag="AC16Y7_00180"
     CDS             3611..4408
                     /gene="rlmB"
                     /locus_tag="AC16Y7_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR5707827.1"
                     /translation="MPDQKYKHSKQFSQGGKGKKGKRRGSPRNAGGEPIQLFGLHAVE
                     AALANPDRPLIGLRATENAAHRLGTLIAERGVQVDTMLPKDLDRLLGPDTVHQGVLLE
                     AGPLPLRTLDDVDPNGILLVLDQVTDPHNVGAALRSAAAFGASGLVLTERHSPPLSGV
                     LAKAASGALDLIPVVAVKNLAQALTELGTRGVMRIGLAEDAETTLEDASLSRPLALVL
                     GAEGKGLRQLTRERCDRMCRISTVNALASLNVSNAAAIAMHWASRQT"
     gene            complement(4506..4910)
                     /locus_tag="AC16Y7_00185"
     CDS             complement(4506..4910)
                     /locus_tag="AC16Y7_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707828.1"
                     /translation="MRILLSLVAVVAVAVGFSLISAAGADARCRVGWGCSAAVLVPAP
                     NPIPLRRMRRPRVVAPCGVAPCAAPVAVAPAPCVAPCGLPPGPTAYRYRAPNYYAYYK
                     QPAYAGECGLSTCNTYWRRNCWYDSFGRRFCD"
     gene            complement(5125..5394)
                     /locus_tag="AC16Y7_00190"
     CDS             complement(5125..5394)
                     /locus_tag="AC16Y7_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707829.1"
                     /translation="MFDVRLGLAAIAAVGLLAVLPQSPAQARDGGPAFHHGKRAQSAY
                     CLRRNYWWFYRPYTTKQEDFPRCEPYFHYLEPASGRRAAPSRYSK"
     gene            5552..5788
                     /locus_tag="AC16Y7_00195"
     CDS             5552..5788
                     /locus_tag="AC16Y7_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707830.1"
                     /translation="MATAKQTEELAAQIDAIRADMQNLTSTVSRIANTQMGRAQDKAV
                     ETAQEAEEAIKRNPLQAVAIAAGLGFLFGVFTRR"
     gene            5804..6220
                     /locus_tag="AC16Y7_00200"
     CDS             5804..6220
                     /locus_tag="AC16Y7_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707831.1"
                     /translation="MLRLVLSLISSLQIGARIQDVVERSVRRTVTIVIAIVVLLFALG
                     FGLVAAYQALLLYDFSPLEASGIVAAVLAFLGVLLLLLSPVLAKPKRKEPDLVNAPAE
                     GLAMIDQGLGKSMQQVGPLTLLALAFAAGLLASRRR"
     gene            6352..6528
                     /locus_tag="AC16Y7_00205"
     CDS             6352..6528
                     /locus_tag="AC16Y7_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707832.1"
                     /translation="MLRRIGLMLLASACIAGPANAQAVDVPRPYAVTLPPNANPSPDP
                     QWELGNSEQSSGTG"
     gene            6531..6842
                     /locus_tag="AC16Y7_00210"
     CDS             6531..6842
                     /locus_tag="AC16Y7_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707833.1"
                     /translation="MRADAAILPVAYLWNEDSHYFRTDLGPVPNRTDEGTGWGYQLEG
                     ELRLDIATNWAIGAGVRYWYAETDGDTDFLHRDTTVELNEFSSERFGVFGNVTYRFAT
                     Y"
     gene            6957..7032
                     /locus_tag="AC16Y7_00215"
     tRNA            6957..7032
                     /locus_tag="AC16Y7_00215"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6990..6992,aa:Thr,seq:tgt)
     gene            7134..7793
                     /locus_tag="AC16Y7_00220"
     CDS             7134..7793
                     /locus_tag="AC16Y7_00220"
                     /inference="COORDINATES: protein motif:HMM:NF016830.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HIR5707834.1"
                     /translation="MKEVGTSCLDPLFSDGAAVAGLWKFYTGTGDDGARSGYISVGGT
                     WSGKEYTVVDPQSLAFVPGVGLSLTDRQESWAIFSVIDQPLWTDSSAPNRTLGFRGMY
                     TLTDGEASPVKWSATAALEVSGPFRSRENDTFAVGYFHNELSSGFENTVGPLLSVAAT
                     VANRTPTRIAIEDTDGFEAYYVAQLTPWCALTGDLQVITETLSTEDTKVVTGFRGNLK
                     F"
     gene            7891..>8118
                     /locus_tag="AC16Y7_00225"
     CDS             7891..>8118
                     /locus_tag="AC16Y7_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707835.1"
                     /translation="MAKPELPPGIVSKEEPQTFVEAVGKINATPAFFDALAKQPDDGP
                     TINLNFLRYRPRGDSSRYDLYGAVAGKEIVGV"
BASE COUNT         1615 a         2654 c         2341 g         1508 t
ORIGIN      
        1 aacgacggtg aactccgtct tctcctcagc cgcagcggca tcaccgccac cgccggccgc
       61 agccgcaacc gccacaggag cagcggcgga cacgccccac ttctcctcaa gaatcttagc
      121 cagttcagcg gcctcgagca ctgtcaggcc cgaaaggtcg tctgcaattt tctgaagatc
      181 tgccattgtc ttatcctcgt acgttcaact cgtattgggt tatttctcgg ctcacgccgc
      241 gtccttgttg ccataggctc caaaaacccg cgcaagctgg gcagccgggg caccgaccgt
      301 gcgggcgatc ttgcccgccg gtgcctgaag gaggccaacc agtttgccgc ggagctcatc
      361 gagcgatggc aattcggcaa gcgaattcac tgcgcttgtg tccagaagcg tggtccccat
      421 cgtaccgcct aggatcacga agttttcgtt ccccttggcg aacttgacgg ccaccttcgg
      481 cgccgtgacg ggatcgtcgg aaaatgcgag gcaagtcggc cccttcatca gatccgtgat
      541 tcccttggcg tctgtccctt cgagcgcaag cttcacgagc ctgttcttcg ccactttgac
      601 ccggccgcca gcttctctca tgtcgctgcg cagcttggtc atgtcggcta cggacaggcc
      661 cgcatagtga gccacgacga ccacgccagt ctcggcgaag gcgtcatgaa gcgcagagac
      721 aacttcgcgc ttctcagctc tttccatgct cactccgttt ttttcaaagc cgcgaagtca
      781 tccctcgcgg ccttcggcca cactgtgccg cgagacatgc ggggcaaggc cagaagccac
      841 cccgcgcgta ctgcgcggcg ctctgttgcc tgtcccgccc aagcgcaaaa acgcgcgcga
      901 gccacatacg aggttcagat cgatctcctg gttgcggaaa gcccgctccc atcgaaccgt
      961 tcatctcccg tctcatgcag gcccgaatgg cttaagcggt ctcccgcacc tgcaatctcg
     1021 gacaggtgtg gattacccgg cgaaccggac aacccttcgg ggccgaagcc ccgaaactct
     1081 ctacttaagc gctgccgaga ctggcaggct cgactttgac gccgggcccc atggtcgagg
     1141 tcacggcgat ccgcttcaca aacgttccct tggcgcccga aggcttggcc ttgttcaccg
     1201 catcggtgaa ggcacggatg ttctccacca gcgcctcttc ggtgaagctc gccttgccca
     1261 cgccggcatg gatgacaccg gccttctcga cgcgaaactc cacggcgcca cccttggcgg
     1321 cgccaacggc cgcggcgaca tcggcggtca cggtgccaac tttcgggttc ggcatcaggc
     1381 cacggggccc gagcacctta ccgagacggc caaccagcgg catcatgtcc ggcgtggcga
     1441 tgcagcgatc gaactcgatc acgcctttct gcacctgctc caccagatct tcggcaccaa
     1501 cgacgtcggc accggccttc ttggcctcct cggccttgtc acccttggcg aacacaccaa
     1561 cccgcagctt gcggccagaa ccatgcggca gctgacatac gccgcgcacc atctgatcgg
     1621 agtgacgcgg atcgacacca agattcatgg cgatctcgat cgtctcgtcg aacttggtct
     1681 tggcaccgct cttgatcagt ttcacggcat cctcgatgcc ataaagcttc tccgcttcaa
     1741 ttgcgtctgc ggcggtacgg aaccttttgc caggcttgct catcgtttac tcctgcacct
     1801 caagacccat cgagcgggcg gagccggcga tgatcttcat cgcttgctca acagtgttcg
     1861 cgttcaaatc caccatcttc gtttcggcga tctcgcgcag ctgatcctgg gacaccttgc
     1921 cggcgccggc gcggccaggc tctttggagc ccttgccgat cttcgccgcc ttcttcagga
     1981 gataagacgc cggcggcgtc ttcatctcga aggtgaagga gcggtcggca tagaccgtga
     2041 tcagtgtggg gatcggcgag ccgggctcga gcttctgcgt ggccgcgttg aatgccttgc
     2101 agaactcagg gatgttcacc ccacgctgac ccagtgccgg cccgatcggc ggcgaaggat
     2161 tcgcctgccc tgccggcacc tgcagcttaa tgtaaccgtc gatcttcttc gccatccact
     2221 taagtcctgc gttctacgaa tgcgcgcgtt gtccgcgcgg tcgttaaagc ttctccactt
     2281 gcgcgtattc gagctcgacc ggggtcgccc ggccaaaaat cgaaaccgcc accttgagcc
     2341 gcgcacgctc ctcgtcgacc tcttccacca agccgttgaa cgaggcaaag ggtccgtcgg
     2401 ccacgcgtac ctgctcgccg atctcgaagg tcacggacgg cttcggccgt tccacgccct
     2461 cctgcacctg atgaaggata cggcccgcct cctcctcggt gatcggcatc ggcttattct
     2521 cggtaccgag aaagccggtg accttgggcg tgtccttgat caggtgatag gtctcgtcat
     2581 cgagctccat cttgaccagc acgtagcccg gaaagaactt gcgctcggac gcgaccttcc
     2641 ggccacgccg catctccacg acttcctcca tcggcacgag cacttgctcg aacttgtcgc
     2701 cgaaaccggc ggcggcggcg cgttccttga tggactccgc gaccttccgc tcgaagttcg
     2761 agtaggcgtg gacgatgtac cagcgcatag ccatccggtt agaatctcat tcccaagatg
     2821 gtgcggacga ccgtccccag cacccaatca acgccaagaa agaacagcgc ggccaggatg
     2881 gacatgatga ccaccatgat ggttgtgatg accgtctccc ggcgcgtcgg ccaggtgacc
     2941 ttggacacct cggtgcgcac ctgctggaga aacacaaaag gattcgtttt cgccatctgt
     3001 ttcggtccta aagaccctgc gcattcgcgc ccgggccgcc cttcttcctt tttgagctca
     3061 tggccggcat ctcgcctctc caacctggca ggagtggagg gactcgaacc cccaaccccc
     3121 ggttttggag accggtgctc tagccagttg agctacactc ctatagccgt ctcgccgtcc
     3181 ctaaaattcg cttccaaatc aaatagcttg cagccctaaa ctgttggagc gggtgaaggg
     3241 aatcgaaccc tcatcgtcag cttggaaggc tgctgctcta ccattgagct acacccgcaa
     3301 tccccatccg gctcctgaag aggtggtgga gggggttgga ttcgaaccaa cgtaggcata
     3361 gccaacgggt ttacagcccg tcccctttag ccactcgggc acccctcctt ggaagcaaag
     3421 atggattaaa cggtgatttg accaagcaaa aacaccccgc cgtcggccgg cagggtccgc
     3481 aatttcagcg tttatgagta gccatggggc ctctgtcaac tgtaaatcag gacgagcgga
     3541 ctggaattcc ttggttttcg gtgccttccg caccttgtcg cagtccccca cgccttgagg
     3601 tcgacatccg atgcctgatc aaaaatataa gcattccaaa cagttctccc aaggcgggaa
     3661 aggcaagaag ggcaagcggc gcggatcgcc ccgaaacgcc ggcggcgagc ccatacagct
     3721 cttcggcctc cacgcggtcg aagccgctct cgcgaatccc gatagacccc tgattggcct
     3781 tcgagccacc gagaacgctg cccatcggct gggtaccctc atcgccgagc gcggtgtcca
     3841 ggtggacacc atgctgccca aggatctcga ccggctgctc ggccccgaca cggtgcatca
     3901 gggcgtgctc ctcgaggccg ggccgctgcc gctccggacc ctcgacgatg tggacccgaa
     3961 cggcattctc ctggtgctcg atcaggtcac cgacccgcac aatgtgggcg cggcgctgcg
     4021 gtcggccgcc gccttcggtg catcgggtct ggtcctgacc gagcgtcaca gcccgccgct
     4081 ctcaggcgtg ctggccaagg cggcgagcgg cgcgctcgac ctcattcccg tcgttgcggt
     4141 caagaacctg gcgcaagcgc tgaccgaact cgggacacgc ggcgtgatgc gcatcggctt
     4201 ggcggaggac gcagagacca cgctcgaaga cgcaagcctc agccgcccct tggcgctggt
     4261 gctcggcgcg gaaggcaagg gcttgcgtca gttgacgcgc gagcgctgcg atcgcatgtg
     4321 ccgcatctcg acggtgaacg cgctggcgag cctcaacgtg tcgaacgccg cggccatcgc
     4381 catgcattgg gcgagccgcc aaacctaaag acgcgtgagg cttaaacgaa cgcgagggag
     4441 aaagtgctca cgcaccctcc ccctcgcatc cgggaatcaa ccagctcgcg ctggaagctc
     4501 taacgtcagt cacagaagcg gcggccgaac gagtcgtacc agcagttcct gcgccaatag
     4561 gtgttgcagg tgctgaggcc gcactcgccc gcataggccg gctgcttgta ataggcatag
     4621 tagttcggcg cccgataacg gtatgcggtc gggcccggcg gcaggccgca cggcgcgaca
     4681 catggcgcgg gcgcaacagc caccggagcc gcgcaaggcg caacgccgca tggcgcgaca
     4741 acccgtgggc gccgcatgcg gcgcagcgga atgggatttg gcgccggaac gaggacggcg
     4801 gcactgcagc cccaacccac ccggcaacgc gcgtcggcac cagccgcgga gatcaggcta
     4861 aagccgaccg caaccgccac aacggcgaca agactcaaca ggattctcat ttgtaactcc
     4921 actcggaacg agggggcgcc ccatgctggc gccggccagt ggtaggctgc ctaaagagca
     4981 aggagcgcaa gtatcagcaa ggttaacaga ggcctgcccg gcccaagaaa aggattacag
     5041 acacactaac gactggcgca aaatgataaa cttgcgctga atatcagtaa ttctaagatc
     5101 agttagagaa ggtgtcgcgt aagactactt ggagtagcgc gacggcgcgg cgcggcgtcc
     5161 cgatgccggc tccaggtagt ggaagtacgg ctcgcagcgg gggaagtctt cttgcttggt
     5221 ggtgtagggc cggtagaacc accagtaatt ccggcggagg caataggcac tctgggcgcg
     5281 cttgccgtga tggaaggccg gcccgccatc gcgggcctgc gcggggctct gcgggaggac
     5341 ggccaacaga ccgacggcgg caatcgcagc cagacccagc ctcacgtcga acattgccga
     5401 attccccctg cggcacccct cttaagcaat cggactctaa acgatgaaag tccctgcggc
     5461 aacgcaaggc tccagcaaga ggtgctgtgc aagacatgct cgtgtatggt tcgttaagaa
     5521 gcctgaccta accattttca gggagacccc catggcaacc gccaaacaga ccgaagagct
     5581 ggccgcacag atcgacgcga tccgggcgga tatgcagaac ctgacctcga ccgtcagccg
     5641 catcgccaac acgcaaatgg gccgcgccca ggacaaggcc gtggagaccg cgcaagaggc
     5701 tgaagaagcc atcaagcgga acccactcca ggccgtggcc attgccgccg gccttggctt
     5761 cctgttcggc gtcttcaccc ggcgctagtc gaggacgatc tccatgcttc gtctcgtgtt
     5821 gtcgctgatc tcgtctttgc agatcggcgc gcgcatacaa gatgtcgtcg agcgctcggt
     5881 ccggcggacc gtgaccatcg tcatcgccat cgtcgttctg ctgttcgcgc tcggcttcgg
     5941 tctggtcgcg gcctaccagg cgcttctcct ctacgacttc tcgccgctcg aggcgtcggg
     6001 gatcgtggca gccgtgctcg cctttctcgg cgtgctcctc ctgctcctca gcccggtcct
     6061 cgccaagccg aaacgcaaag aaccggacct ggtcaacgca ccggccgaag gcctggccat
     6121 gatcgatcag ggcctcggca agtccatgca gcaggtgggt ccgctgaccc tgctcgccct
     6181 cgccttcgcc gccggccttc tggccagccg ccgccgctaa ccctcaagtt ttccgtgcga
     6241 tagagggcct caacgtccca tttcggcgca cgtcttaagc tctccttaac cagccgccag
     6301 ccaccttggg gaccagcgaa aacgtgagtc taccaagggg cgaggcggaa catgctacga
     6361 cgcataggtc tgatgctgct ggcttcggcc tgcatcgcag ggcctgccaa cgcgcaagcc
     6421 gtcgacgtgc cgcgccccta cgccgtcacc ctgccgccca acgccaaccc ctcccccgat
     6481 ccgcaatggg agttgggcaa ctcagagcaa agctctggga ccggctgagt gtgcgcgccg
     6541 acgccgccat cctccccgtc gcctatctct ggaacgagga cagtcactac ttccgcactg
     6601 atctcggccc cgtgcccaac cgcaccgacg aaggcacagg ctggggctat caactcgaag
     6661 gcgaactaag gctggatatt gcgaccaatt gggccatcgg cgcaggggtg cgctattggt
     6721 atgccgagac ggacggcgac accgactttc ttcatcgcga cacgaccgtg gagctcaatg
     6781 agttctcgag cgagcgcttc ggcgtgttcg gcaacgtcac ctaccgcttc gccacgtact
     6841 agccgcgggc tctcacgcca cgccgcttga gcaggactgc aaaagtggga taaaggggct
     6901 tggagtgcag cgcgcctcgc cccagatcag ggtcatgcag aggccaagca tcagatgccg
     6961 gcttagctca gttggtagag cggctgattt gtaatcagta ggtcgggggt tcgaatcctc
     7021 cagccggcac caaatcaaca atatatatca gataattaga tgcaacagca agaagtggct
     7081 ccagctcttc tcagtctgtg tccgcaagat gtccgcaaat cacgaccaga accatgaaag
     7141 aagtggggac atcctgccta gaccccctct tttcagatgg cgctgccgtc gcgggcttat
     7201 ggaagttcta caccgggaca ggcgacgatg gtgcgcgctc gggatacatc agcgttggcg
     7261 gcacatggag cggcaaagaa tatacagttg tagatccaca aagcttggct ttcgttccag
     7321 gcgtgggcct gtctctaacg gaccgacagg agtcctgggc gattttttcc gttatcgacc
     7381 agcctctatg gacagattct tcagctccta acaggacgct tggcttcaga ggcatgtaca
     7441 cactcaccga tggcgaagcg agccccgtca agtggagcgc cactgccgct ctagaagtga
     7501 gcggcccatt ccgaagccgg gagaatgaca cgttcgccgt cggttacttc cataacgaat
     7561 tgagcagcgg ttttgagaac acagtcggac ctcttctttc ggtggcggca accgtcgcaa
     7621 acagaacgcc gacaagaatt gcgatcgaag acactgatgg ctttgaagcc tattacgtcg
     7681 cccagcttac gccctggtgc gcactaaccg gagacctgca ggtgataacc gagaccttga
     7741 gtacggaaga cactaaagtg gtgaccggct tccggggaaa tctcaagttc tgagtatcac
     7801 gtcggcgccg cgcggcaggt tgcaggctca gcgcgcttgt ccaaacggca agtagccgag
     7861 ccgcccgtaa aaatctcgaa ggagcaataa atggcaaagc cagaacttcc gcctggtatc
     7921 gtttctaagg aggagccgca aacctttgtt gaggcggttg gaaagatcaa cgccacgccg
     7981 gcatttttcg acgccctcgc caagcagcca gatgatgggc cgaccatcaa cctcaacttt
     8041 ctgcgctacc ggccacgggg agacagctca cgatatgatc tttacggcgc cgtggccggc
     8101 aaagagattg tcggagtc
//
LOCUS       DBNHVU010000005         4717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_4128, whole genome shotgun sequence.
ACCESSION   DBNHVU010000005 DBNHVU010000000
VERSION     DBNHVU010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4717
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_4128"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="AC16Y7_00230"
     CDS             <1..1375
                     /locus_tag="AC16Y7_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011505158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5707836.1"
                     /translation="LGKAGRASTATDPAPMSMFETVINLKPEAEWRDGMTIDGLIAKL
                     DEALQFPGVSNSWTMPIKARIDMLSTGIRTPVGIKVIGRDLGEMEKLAREIEVAVRTV
                     PGTTSAYAERATGGYYLNIDPDRAQLARYGLMVGDVQSVIATALGADPVTTTVEGRER
                     YNVAVRYPRDYRDNPQAIASEVLIPTPAGGTVPLSEVATISLAQGPSMIRTENAQLAL
                     YIFVDFRDRDIGNYVADAQKAVAAQVDFPPGYYVTWSGQFEYLERAKKRLQIVVPLTV
                     LIIFVLLYLNFRRLTETLIVLLSVPFAAVGGLWLMWWLDFNFSVAAVTGFIALAGVAA
                     ETGVVMLIYLDNALKERRRERESEGHTLSAGDLHAAIMAGAVGRVRPKIMTVTAIMAG
                     LLPIMWAHGTGAEVMQRIAVPMIGGMVSSTALTLIVIPVIYALVKGVGLRPLKDAQPK
                     GLLRQSS"
     gene            complement(1417..2577)
                     /locus_tag="AC16Y7_00235"
     CDS             complement(1417..2577)
                     /locus_tag="AC16Y7_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transporter family protein"
                     /protein_id="HIR5707837.1"
                     /translation="MATAAIVQAFAFGAAADSTQATGAASEGNPTPGSFDILFADGEA
                     KRAPRADGHGPIGVMGDHRHHEGDLMLSYRYMRMQMEGNLIGDKSVSPDTIVTTVPNR
                     FFGRPMQPPTLRVVPTEMTMDMSMFGAMYGLTDRVTLTAMLPLTDKEMSHITYRGPMG
                     TTRLGTFTTDSEGIGDFVFGGLVGLYDSKSAEGEQHLNLILNVSAPTGSITEDGRVLT
                     PMGGTPLVRLPYAMQLGSGTWDFLPGVTYTARRRNLSFGAQYRGNFRLEDENSEGYAL
                     GDLHQATGWAAFEWVPWVSNSVRLAYQTQGDISGIDTNIVAPVQTANPDFYGGDRLDL
                     LFGVNLVGQSGAVCGHRLAAEFGFPVHQDLNGPQMETDYTFTLGWQKALGDC"
     gene            complement(2713..3069)
                     /locus_tag="AC16Y7_00240"
     CDS             complement(2713..3069)
                     /locus_tag="AC16Y7_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707838.1"
                     /translation="MASAFALLGVLLYTALVPGHVISQATAFLLADEFGTALEMSCHE
                     GMEVGSSAPGEPSVPQKKCPFCKGYAPFMTALAGMSDAGTIDAERTTLQFVVADDQAV
                     QRMSRRPHNRGPPLEL"
     gene            3243..4229
                     /gene="glk"
                     /locus_tag="AC16Y7_00245"
     CDS             3243..4229
                     /gene="glk"
                     /locus_tag="AC16Y7_00245"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HIR5707839.1"
                     /translation="MSETSKIPSHALVGDIGGTNARFAIADLATLALSHVGGSPTASH
                     PTLADAIRDYLKGVDQKIEHAGIAVAGPLLDDTVKLTNAAWTFCQSSLAKDAGLKTAH
                     VFNDFEAQAYCVPVLAPEERHQLGGTEPVEHAPKVVLGPGTGLGVAGLVWSASGWVAV
                     AGEGGHVTLAAENERELALIERMRKGLVRLSVERALSGPGLAELYQAIAASRGFGDSP
                     LSPAEIEHLAISGEDEMAEEALDFFVSWLGRFAGDMALVFGARGGAYLGGGIAPKMLS
                     RLQQDDFREAFERKGRMTPFVAPIPVYVVVADNPGLRGAAAGLRTRLKSAVT"
     gene            complement(4259..>4717)
                     /gene="greA"
                     /locus_tag="AC16Y7_00250"
     CDS             complement(4259..>4717)
                     /gene="greA"
                     /locus_tag="AC16Y7_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR5707840.1"
                     /translation="MTAEGYAALEIEIKTLKTVERPRIIKLIAEARSHGDLSENAEYH
                     AANELQGITEARIADLEDKLSRADIIDVSKLKGDQVMFGATVTLVDEDTDEKVKYRIV
                     GEVEADVKEGRISITSPIARALLGKRKKDVVEVSTPGGGKSYEIMRVAFK"
BASE COUNT          853 a         1463 c         1531 g          870 t
ORIGIN      
        1 actcggcaaa gccggacgtg cttcaacggc aacggatccg gcgccgatga gcatgttcga
       61 gacggtaatc aacctcaagc cggaggccga atggcgtgat ggcatgacca tcgatggtct
      121 gatcgccaag cttgatgagg ctttgcagtt cccgggcgtc tccaactcct ggaccatgcc
      181 tattaaggcg cgcatcgaca tgttgtcgac gggcatccgc acgcctgtcg gcatcaaagt
      241 catcggccgt gacctcggtg agatggagaa actagctcgc gagatcgagg tcgccgtccg
      301 gaccgtgcca ggaacgacca gcgcttacgc cgagcgggcc acgggcggtt actacctcaa
      361 catcgatccc gaccgcgcac agctcgcgcg ctacgggctt atggttggcg acgttcagag
      421 cgtgatcgcc accgcgctcg gtgccgatcc cgtcacgacc actgtggagg gcagggagcg
      481 ctacaacgtg gccgtccgct atccgcgtga ctaccgtgac aatccgcaag ccattgccag
      541 cgaggtgctt attccgacgc ccgccggcgg cacggtgccc ttgagcgagg tggcgacgat
      601 cagcctggcg caagggccgt ccatgatccg cacggaaaac gcacagctcg cgctctatat
      661 ctttgtggat ttcagggatc gcgacatcgg caactatgtc gccgatgcac aaaaggccgt
      721 cgcggcacaa gtcgactttc cgcccggcta ttacgtcacc tggagcggtc agttcgaata
      781 tctggagcgc gccaagaaga ggcttcagat cgtcgtgccg ctgacagtgc tcatcatctt
      841 cgtgctgctc tatctaaact tccggcgact gacggagacg ctcatcgtcc tgctgtcggt
      901 gccattcgcg gcggtcgggg ggctgtggct catgtggtgg ctggacttca acttctcagt
      961 cgctgctgtc acgggcttca tcgcgctggc tggcgttgcg gccgagaccg gcgtcgtcat
     1021 gctgatctac ctcgacaacg cgctcaaaga gcgcaggcgc gagcgggaga gcgaaggcca
     1081 cacactcagt gccggcgacc tgcacgcggc catcatggcc ggtgcggtcg ggcgcgtgcg
     1141 gcccaagatc atgacggtga ccgcgatcat ggcggggctc ttgcccatca tgtgggcgca
     1201 tggcacgggc gcggaagtga tgcagcgtat cgccgtgccg atgatcggcg gcatggtgtc
     1261 gtcgacagcg ctgacgctga tcgtaatccc ggtcatctat gcgttggtca aaggcgttgg
     1321 cttgcgccct ctaaaggacg cgcagccgaa aggtctgttg cggcagagct cctgacttct
     1381 gagtcagggc ctgccgcaac atttgcttgt ctgccgtcag cagtcgccga gcgccttctg
     1441 ccagccgagc gtgaacgtat aatccgtctc catctgcggg ccgttcaggt cctggtggac
     1501 ggggaagccg aactcggccg cgagccgatg cccgcaaaca gcgccgctct ggccgaccag
     1561 attgacgccg aacaagaggt ccagtcggtc cccgccgtag aagtctggat tggccgtctg
     1621 caccggcgcg acaatgttcg tatcgatccc gctgatgtca ccttgggttt ggtaggcgag
     1681 acgcaccgag ttgctgaccc aagggaccca ctcaaacgct gcccagcctg ttgcctgatg
     1741 caaatcgcca agcgcgtagc cttcgctgtt ctcgtcttcg aggcggaaat tgccccgata
     1801 ctgcgcaccg aacgacagat tgcggcgccg cgccgtgtag gtgacgccag gcaggaaatc
     1861 ccacgtgccc gagccgagct gcatggcata gggcaggcgc accagaggcg tgccgcccat
     1921 cggagtgagg acccggccgt cctccgtgat cgagcccgtc ggcgcgctca cgttcaaaat
     1981 gaggttcaaa tgctgctcgc cttccgccga cttggagtcg tagagcccga cgaggccgcc
     2041 gaacacgaaa tcgccgatgc cttcgctgtc ggtggtgaac gtgccgagac gcgtcgtgcc
     2101 catcgggcct ctataggtga tgtgactcat ctccttgtcg gtgagcggca gcatcgccgt
     2161 cagcgtgaca cggtcggtga ggccgtacat ggcgccgaac atgctcatgt ccatggtcat
     2221 ttccgtcggc accacgcgca aggtcggcgg ctgcatgggc cggccgaaga agcggttggg
     2281 tacggtcgtg acaatggtgt ccggcgaaac gctcttgtcg ccgatgaggt tgccttccat
     2341 ctgcatccgc atgtagcggt aggaaagcat caggtcgcct tcgtggtggc ggtgatcgcc
     2401 catgacgccg attggcccgt ggccgtcggc gcgcggcgcg cgcttcgctt cgccgtcggc
     2461 aaacaagatg tcgaagctgc cgggcgtggg attgccttcg ctagcggcgc cggtggcttg
     2521 tgtgctgtct gcagccgcgc cgaaggcaaa tgcctggacg atggctgcgg tggccaaggc
     2581 cggcagcaac cgccttggtg cggatgatat gcaaaacatt tgtcgaactc cctgcgtgtt
     2641 ggatcgatcc aaccacggca cgccacacgc aagtctcaag cgtgcggccc ccgcggacgg
     2701 acatcgaaaa catcagagtt cgagaggtgg tccccggtta tgcgggcgcc gggacatgcg
     2761 ctgcaccgcc tggtcgtcgg caacgacgaa ttgcagcgtg gtccgttcgg catcgatcgt
     2821 gccggcgtcg ctcatgccgg caagggcggt catgaagggc gcatagccct tacagaacgg
     2881 gcatttcttc tgaggaaccg aaggctcgcc gggggcagag ctgcccacct ccatgccttc
     2941 gtggcaggac atttccagcg ctgtgccgaa ctcgtcggca agcaggaacg ccgtcgcctg
     3001 cgatatcacg tgccccggaa cgagcgccgt ataaagcaga acgccgagaa gcgcgaaagc
     3061 gctcgccaag cggcgatacc gatggaagtc ccccaactcc atgcctcaat aatagccagg
     3121 agcctagggg agcgaaagcc ggttgaacgg tcagagtgac gcaagcgggc aacactcgca
     3181 ggggggccaa aaatggggta ccaccatgtc catggtcaat tcgcaccggg gctttgcgga
     3241 ccatgagtga gacaagcaaa attccgtcgc acgcgctcgt cggcgacatt ggcggcacca
     3301 atgcgcggtt cgccatcgcc gatctggcaa cgctcgcctt gagccatgtg ggcggctcgc
     3361 cgaccgcatc gcacccgacg ttggcggacg caatccgcga ctatctcaag ggcgtggacc
     3421 agaagatcga gcatgcaggc atcgcggtgg cggggccatt gctggacgac acggtcaagc
     3481 tcaccaacgc cgcgtggacc ttctgccaga gcagcttggc gaaggacgca gggctgaaga
     3541 cggcgcatgt cttcaacgat ttcgaggcgc aagcctattg cgtgccggtt ctcgcgcccg
     3601 aagagcgcca tcagctcggc ggcaccgagc ccgtcgagca tgccccaaaa gtcgtgctgg
     3661 gtccgggcac agggctcggc gtcgccggtc tcgtctggtc ggccagcggc tgggtcgcgg
     3721 tggcgggcga aggaggtcac gtcacgcttg ccgccgagaa cgagcgcgag ctcgccctga
     3781 tcgagcgcat gcgcaaaggc cttgtgcgcc tatccgtgga gcgcgcgctg tccgggcctg
     3841 ggctcgccga gctttaccaa gccatcgccg cctcgcgcgg cttcggcgat tcgcccctat
     3901 cgccggcgga aatcgaacat ttggcaatct ccggcgagga cgagatggcc gaggaggcac
     3961 tcgatttctt cgtcagctgg cttggccggt tcgcgggcga catggcgctg gtatttggcg
     4021 cgcgcggggg tgcctatctc ggcggcggta tcgcgccgaa aatgctgtct cgtctccagc
     4081 aagacgattt ccgggaagcc ttcgagcgca agggtcgtat gacgccgttc gtcgcgccga
     4141 tcccggtcta tgtggtggtg gccgacaatc cggggctcag aggtgccgcg gctggcttgc
     4201 gaacgaggct gaagagcgcc gtaacctaga gaaacgattc agtttttggc aggcaggtct
     4261 atttgaacgc gacccgcatg atctcgtagg acttgccgcc gccgggcgtc gacacttcga
     4321 ccacgtcctt cttgcgcttg ccgagaagcg cccgcgcgat cggcgaggtg atggagatcc
     4381 ggccctcttt cacgtcggcc tcgacctccc cgacgatgcg gtacttgacc ttctcgtcgg
     4441 tgtcctcgtc gaccagcgtg acagtcgcgc cgaacataac ctgatcgccc ttgagcttgg
     4501 acacgtcgat gatatcggcg cgcgacagct tgtcctcaag gtcggctatg cgggcctcgg
     4561 tgatgccctg cagctcattg gcggcgtggt attcggcgtt ttccgagagg tcaccatgag
     4621 agcgagcctc ggcaatcaat ttgatgatac gcggccgctc aacggtcttg agcgtcttga
     4681 tctcgatctc gagggcggca tagccctccg cggtcat
//
LOCUS       DBNHVU010000006         6012 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1194611, whole genome shotgun sequence.
ACCESSION   DBNHVU010000006 DBNHVU010000000
VERSION     DBNHVU010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1194611"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..122
                     /locus_tag="AC16Y7_00255"
     CDS             <1..122
                     /locus_tag="AC16Y7_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR5707841.1"
                     /translation="VDAIVAAAKTGQIGDGKIFVVPVEQTVRIRTGETNESAL"
     gene            140..1492
                     /locus_tag="AC16Y7_00260"
     CDS             140..1492
                     /locus_tag="AC16Y7_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR5707842.1"
                     /translation="MTTLAKYLFGVVAALAIAALYHIDPALAQEAAAEGAEPLAEAAE
                     ETAKLDTGDTAWMLTSTVLVLMMAIPGLALFYGGMVRKKNVLSVVMQCFAIVCLLSIT
                     WFVVGYSLAFTDGGSLNSYIGGLSKMFLNGVGVDTLSGTIPESVFFTFQMTFFAITPA
                     LIVGAFVDRMKFSAVCIFMVLWALIVYAPVCHWVWGGGFLSEDGVLDFAGGTVVHINA
                     GIAGLVCALFLGKRVGYGTENMAPHNLVLSLIGASLLWVGWFGFNVGSALAANAGAGM
                     VMVTTQIATAAAALSWMFIEWLIHKKPSVLGIISGAIAGLVAITPACGFVDGFGALWI
                     GIAAGLICFFVSTTVKRSLGYDDSLDVFGIHGVGGIVGALLTGVFAVEAIGGTAGLLE
                     GNAGQVLTQLWGIVATIVWCAIATFVILIITNILVGVRVSQAVEVEGLDINLHGEVVQ
                     "
     gene            1652..2860
                     /locus_tag="AC16Y7_00265"
     CDS             1652..2860
                     /locus_tag="AC16Y7_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5707843.1"
                     /translation="MSVLGETNSLDAIIRSPFLRLNALLEDIEPGAPAIHFHLGEPKA
                     SLPSFLAPVLNEHLVEFGRYPPIKGTPGLREAISAWMGRRYPALRGVIDPEHHVLPLN
                     GSREGLFSAVFPAKDRRAEIADPAVLIPNPFYQVYAAATAVAGAEPAFLPAGPEGGFL
                     PKLDEIDEAVLSRTIGFYLCSPSNPEGAVANGEYLAQAIALARRHDFLLFADECYSEI
                     YGDAPPPGALEVAQAETGSLDNIVAFQSLSKRSGLPGLRSGFVAGDPSFLAAFGRFRT
                     VACPQIPLPIQHVSAAAWAEETHVEAARALYEANFDVADSILKGRYGYRRPAGSFFLW
                     LNFADFGGGEAAATTLWKGCGVRVLPGAYLTYAQANQANPGQDFVRLALVHDADTTRE
                     GLARIVATLG"
     gene            2916..5336
                     /locus_tag="AC16Y7_00270"
     CDS             2916..5336
                     /locus_tag="AC16Y7_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015341497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE family DNA translocase"
                     /protein_id="HIR5707844.1"
                     /translation="METGLRRLLFGTYGFFLIAGACAVWLSLATWSVQDPSLNNATRA
                     VPRNLLGDWGAVIADLGIQSLGLAAVFLFLPLAAWGWHLVARTTPNSRKWRLIAWPAS
                     ALMLAAAFAALPQPKSWPLPNGLGGILGDFIMAGAHIIGPFLPEAAVAFVAGLIFFII
                     GFWLLMFASGTSTSQLIALWAPRSSAAGEWANAWIGAVLHFVMHSFGALRRMFRRKPR
                     EADGYDAAYDEDEDEDWAEEPPAVDTHGRIEPSFGPAPLIDDETYDADYDDDAPDYRI
                     TRLEQKEQKKTKKRHESIHSQYSDAPFNPPALRLLQQKPRSARNREVSDEVLQENARE
                     LEGVLQDFGVKGEITNVRPGPVVTLYELEPAPGTKSSRVIGLADDIARSMSAIAARVA
                     VVPGRNAIGIELPNDQREMVMLRELFESPDFRDTDARLALSLGKTIGGEPMIADLSRM
                     PHLLIAGTTGSGKSVGINTMILSLLYRLSPEQCKFIMIDPKMLELSVYDGIPHLLAPV
                     VTDPKKAVVALKWTVREMEERYKRMSKLGVRDIKGYNARVLQAESRGEVLTRTVQTGF
                     DRETGQAIYEQEEMDYEAMPYIVVIVDEMADLMMVAGKDIEAAVQRLAQMARAAGIHL
                     ITATQRPSVDVITGTIKANFPTRISFQVTSKIDSRTILGEQGAEQLLGSGDMLYMAGG
                     GRIIRVHGPFVSDEEVEKVVRHLKKQGVPAYLDAVTDDSESEDEDGEGGSGYGNSGDD
                     IYDQAVAVVLRDRKVSTSYIQRRLGIGYNRAATLIERMENEGLIGAANHAGKREILVG
                     ENEGTAPY"
     gene            5447..>6012
                     /locus_tag="AC16Y7_00275"
     CDS             5447..>6012
                     /locus_tag="AC16Y7_00275"
                     /inference="COORDINATES: protein motif:HMM:NF015510.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA family protein"
                     /protein_id="HIR5707845.1"
                     /translation="MTSRRAAGILLGVPIALGLSMSAAPAQDGSTVVPKSSVPTTPVT
                     GAAPAGAAAGSPGDALRPSIGEEVVPPEDAAPVSEIKPAAVDEGESWEAAVEAANAPT
                     PLIGAEQSKAVQQINAYFNGITNMQGRFEQIDPNNKRTNGKFYVERPGKVLFDYAPPS
                     ELRILADGHSLAIEDSDLKTVEKYPIKST"
BASE COUNT         1081 a         1903 c         1868 g         1160 t
ORIGIN      
        1 ctgtggacgc catcgtggcc gcggcaaaga ccgggcagat cggcgacggc aagatcttcg
       61 tcgttcccgt cgaacagacg gtgcgcatcc gcaccggcga aaccaacgaa tcagcactgt
      121 gagaggggga gaattgaata tgacgaccct tgccaaatat cttttcggcg tggtcgctgc
      181 gctcgcaatt gcggcgctct accacatcga tcctgcactc gcccaagagg cggcggccga
      241 gggagcagag cctctggcgg aagctgccga ggaaactgca aaactcgata cgggtgacac
      301 ggcctggatg ctgacatcca ctgttctcgt cctgatgatg gccattccgg gcctcgcgct
      361 cttctacggc ggcatggtcc gcaagaagaa cgtgctgtcc gtggtgatgc agtgcttcgc
      421 gatcgtctgc ttgctgtcga tcacgtggtt cgtcgtcggc tactcgctcg cgttcaccga
      481 cggtggctcg ctcaactcct atattggcgg tctgtcgaag atgttcctga acggtgtcgg
      541 tgtcgatact ctgagcggaa ccattccgga atcggtcttc ttcaccttcc agatgacgtt
      601 cttcgccatc acgccggcgc tgatcgtcgg tgccttcgtg gaccgtatga agttctcggc
      661 tgtctgcatc ttcatggtgc tttgggccct gatcgtctac gccccggttt gtcactgggt
      721 gtggggcggc ggcttcctgt cggaagacgg cgtgctcgac ttcgctggcg gtacggtggt
      781 gcacatcaac gccggtattg ccggtctagt ctgcgccctg ttcctcggaa agcgcgtcgg
      841 ctacggcacc gagaacatgg cgccgcacaa tctggtcttg agcctgattg gtgcatcgct
      901 tctgtgggtc ggctggttcg gctttaacgt cggctcggct ctcgctgcca acgccggtgc
      961 cggcatggtc atggtcacga cgcaaatcgc gaccgcggct gcggctctgt catggatgtt
     1021 catcgaatgg ctcatccata agaagccgtc cgttctcggc atcatctcgg gcgcaattgc
     1081 cggtctcgtg gcgatcaccc cggcttgcgg cttcgtcgat ggctttggtg cactttggat
     1141 tggtattgct gccggtctga tctgcttctt cgtatcgacg accgtgaaga ggtccctggg
     1201 ttacgacgac tcgctcgatg tcttcggcat ccatggcgtg ggcggcatcg ttggcgcact
     1261 gctgacgggc gtgttcgcgg tcgaggcgat cggtggaacc gccggcttgc tggaaggcaa
     1321 tgccgggcag gtcctcaccc agctctgggg catcgtggcc accatcgttt ggtgcgcgat
     1381 cgcgaccttc gtcatcctga tcatcaccaa catccttgtc ggggtgcggg tgagccaggc
     1441 tgtcgaagtc gagggcctcg acatcaactt gcatggcgag gtggtccaat agacctccgc
     1501 gcaatgtaac gagggacaac aatgacaaag agctaggtcg cctcctccct gacctcagct
     1561 ccaccttgga ccccggcgcc tctcgcgccg gggtctttta cattgtgcca aggcattgcc
     1621 gcaggccgcg tcatcgccta acaccgcgtg catgtcagtc ttgggcgaga ccaactctct
     1681 cgacgcgatc atccgctcgc cgttcttgcg tttgaacgcg ctgcttgagg atatcgagcc
     1741 cggcgcgccg gcgatccact tccatctcgg cgaaccgaag gcgtcgctac cgtcgttctt
     1801 ggcgccggtg ctgaatgaac atctcgtcga gttcgggcgg tacccgccga tcaagggaac
     1861 gccgggtttg cgcgaggcga tcagcgcttg gatgggacga cgctatccgg cgcttcgcgg
     1921 cgtgatcgat ccagagcatc acgtcctgcc gctcaacggc tcgcgcgaag gattgttttc
     1981 ggccgtcttt cccgcaaaag acagaagggc tgagatcgcg gacccggcgg ttctcattcc
     2041 caacccgttc tatcaagtgt acgcggcggc gaccgcagtc gcgggcgctg agcctgcgtt
     2101 cctgccggcg ggtccggaag gcggcttttt gcccaagctg gacgagatcg acgaggcggt
     2161 cttgtcgcgc acgatcggat tctatctctg ctccccatcg aatcccgaag gggccgtggc
     2221 caacggcgag tacctcgccc aggcaatcgc actcgctcgc cgccacgatt tcctgctgtt
     2281 tgccgacgag tgctattcgg agatctacgg cgatgcgccc ccgcccggtg cactggaggt
     2341 cgcccaggcc gagaccggca gcctcgacaa tatcgtggcc ttccaatcgc tatcgaagcg
     2401 atccggcctg cctggcctcc ggtccggctt cgtggccggc gatccgtcat ttttggcggc
     2461 tttcggccga ttccggactg tagcctgccc gcaaatcccg ctgccaatcc agcacgtttc
     2521 ggcggcggcc tgggccgagg agacccatgt ggaggccgcg cgggccctct atgaggccaa
     2581 tttcgacgtg gccgattcga tcctgaaggg ccgctacggc taccgccggc ccgcgggaag
     2641 cttcttttta tggctgaatt tcgccgattt cggcggtgga gaggccgccg cgacaaccct
     2701 ttggaaaggg tgtggtgtca gagtcctccc aggggcctac ctcacgtacg cgcaagccaa
     2761 ccaggccaat cccggccagg actttgtcag gcttgcgctc gtgcatgacg cggacacaac
     2821 gcgggaaggc ttagcgcgca tcgtcgccac actgggttag agggatggca ccaagacgta
     2881 gatccaaatc acagcggcgt cttttaccga gcgccgtgga gaccggcctg cgccggctcc
     2941 tattcggcac gtacggcttc tttctgatcg ccggcgcctg cgccgtatgg ctgagcctgg
     3001 ccacctggtc ggtccaagat ccgagcctga acaacgccac ccgggcggtg ccccgcaatc
     3061 tgcttggcga ctggggcgcg gtgatcgccg atcttggcat ccaatcctta gggctcgccg
     3121 cggtcttcct gtttctgccg cttgccgcct ggggctggca tctcgtggcc cgcaccacgc
     3181 cgaatagccg caaatggcgc ctgatcgcct ggcccgcctc ggccctcatg ctcgccgctg
     3241 ccttcgccgc cctgccccaa ccgaagagct ggcccctgcc gaacgggctc ggcggcattc
     3301 tcggcgactt catcatggcc ggcgctcaca ttatcgggcc cttcctgcca gaagccgcgg
     3361 tcgcattcgt tgccgggctc atctttttca tcatcggctt ctggcttctg atgttcgcta
     3421 gcggcaccag cacctcgcag ctcattgctt tgtgggcacc gcgatcctcg gcggcaggcg
     3481 agtgggccaa tgcctggatc ggcgcagtcc tgcatttcgt catgcacagc tttggcgcgt
     3541 tacgccgcat gttccgccgc aagccgcgcg aagccgatgg ctatgacgcg gcctatgacg
     3601 aggacgaaga cgaggattgg gctgaggagc cgccggcggt cgacacccac ggccgcatcg
     3661 agccctcctt cggcccggcg cctctcatcg acgatgagac ttacgacgcg gactacgatg
     3721 acgatgcgcc ggactaccgc atcacgcggc tcgagcagaa agagcagaag aagacgaaga
     3781 agcggcacga gagcattcat tcgcaatact ccgacgcacc cttcaatccg ccggcgctga
     3841 ggctccttca gcaaaagccg cgctccgcgc gaaaccgcga ggtaagcgac gaggtgttgc
     3901 aggagaacgc gcgcgagttg gagggtgtgc ttcaggactt cggcgtgaag ggtgaaatca
     3961 ccaatgtgcg ccccggcccc gttgtcacgc tctacgaatt ggagcccgca ccaggcacaa
     4021 agtcgtcgcg cgttatcggc cttgccgacg acatcgcacg ctccatgagc gccatcgccg
     4081 cgcgcgtggc ggtggttccc ggacgcaacg ccatcggcat agaactgccc aacgatcaac
     4141 gcgagatggt aatgcttcgc gagttgttcg aatcgcctga ttttcgcgat acggacgcgc
     4201 ggctggcgct gtccctcggc aagacaatcg gcggcgagcc gatgattgcc gatctatcac
     4261 gcatgccgca tttgctcatt gccggtacca cgggctcagg caaatcagtc ggcatcaaca
     4321 ccatgatctt gtcgctgctc tatcggcttt cgccggagca atgcaaattc atcatgatcg
     4381 atccgaaaat gctggagctg tccgtctatg acggcattcc gcatctgctt gcgccggttg
     4441 tcacagatcc gaagaaggcc gtcgtcgcgc tgaagtggac cgtgcgcgag atggaagagc
     4501 gctacaagcg catgtccaag ctcggcgtgc gcgacatcaa gggctacaac gcccgcgtcc
     4561 ttcaggccga gtcgcgcggc gaggtgttga cgcggaccgt gcagaccggg ttcgaccgcg
     4621 aaaccgggca ggccatctac gaacaagagg agatggatta cgaggcgatg ccgtacatcg
     4681 tcgtgatcgt cgacgagatg gcggacctca tgatggtcgc gggcaaagac atcgaagcgg
     4741 cggtgcagcg ccttgcgcaa atggcgcgcg cggcgggtat acatttaatc acggcaacgc
     4801 agcggccgag cgtcgacgtc attaccggca ccatcaaagc gaacttccca acgcgcatca
     4861 gcttccaggt cacctcgaag atcgacagcc gcacgatcct cggtgagcaa ggcgctgagc
     4921 agctgctcgg ttccggcgac atgctctaca tggcaggcgg cggccgtatc atccgcgtcc
     4981 atggcccctt cgtctccgat gaagaagtgg agaaggtggt gcgccacctc aagaagcagg
     5041 gcgtaccggc atacctcgat gccgtgacgg acgactccga gagtgaggac gaggacggcg
     5101 agggcggctc cggatacggc aatagcggcg acgacattta cgaccaggcg gtggcggtgg
     5161 tcctgcgcga ccgcaaggtg tcgacgagct atatccaaag gcgcctcggg atcggctata
     5221 accgggccgc gacgctcatt gagcgcatgg agaacgaggg tctcatcggc gctgccaacc
     5281 atgctggaaa gcgcgaaatc ctggtcggcg agaacgaggg caccgcacct tattaggtgc
     5341 gttgacttgc cgccgccaag ccgccaagaa gttgaccaga tttggcggaa tgatctatcg
     5401 aatacctgcc aatcctttcc cgagctaaat gcgtcatggg gggtttatga ctagccgcag
     5461 agccgccgga attctccttg gcgtgcccat tgctctggga ctctccatga gcgccgcgcc
     5521 ggctcaggac ggctcgaccg tggtcccgaa gtcgtccgtg ccaaccacgc cggtcactgg
     5581 agccgcccca gccggggccg ccgccggctc tccgggtgac gcgctccgcc cttcgatcgg
     5641 cgaggaggtc gttcccccgg aagacgcagc gcctgtcagc gaaatcaagc cagccgcggt
     5701 cgacgagggc gaaagctggg aggcggccgt cgaggcagcc aatgcgccga cgcccctcat
     5761 cggcgctgag cagtccaagg cggtgcagca gatcaacgct tatttcaacg ggatcaccaa
     5821 tatgcagggc cgtttcgagc agatcgaccc caacaacaag cgcaccaacg gaaagttcta
     5881 cgtggagcgg ccgggcaagg tcctgttcga ctacgccccg cccagcgaac tgcgcatcct
     5941 ggctgacgga cactccttgg cgatcgagga cagcgacctg aagacggtcg agaaatatcc
     6001 gatcaagtcc ac
//
LOCUS       DBNHVU010000007         9926 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_5983, whole genome shotgun sequence.
ACCESSION   DBNHVU010000007 DBNHVU010000000
VERSION     DBNHVU010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 9926)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9926)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9926
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_5983"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /locus_tag="AC16Y7_00280"
     CDS             <1..957
                     /locus_tag="AC16Y7_00280"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HIR5707846.1"
                     /translation="KQAVFGLDFPNPIGMAPGFDKDARVPHQLLEMGFGFVEVGTLTP
                     RAQSGNPAPRLFRSHRDRAIVNRLGFNNEGQDAALTRLARRPRGIVGVNLGANRDSTD
                     RIADYADGITRMAPVADYFTINISSPNTPGLRDLQAPRKLDALLGKVQEARKALGPKA
                     PPLLVKLAPDLADADLPEVAAVIEQNEADGIIVSNTTLARDNLRDETLLREAGGMSGR
                     PAFLRTTRMLARVHQATEGRLPLIGVGGLDSGEAALAKIEAGASLIQLYTALVFEGPG
                     LIGRIKDTLVRAMARENADNLKPLIGRRAGEWSEKELPDVVV"
     gene            complement(973..1491)
                     /locus_tag="AC16Y7_00285"
     CDS             complement(973..1491)
                     /locus_tag="AC16Y7_00285"
                     /inference="COORDINATES: protein motif:HMM:NF013003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR5707847.1"
                     /translation="MKPKTYFLCTVVFLASVAADASLSLAFDKAKLDTFLTTNACKYC
                     DLSEADLSGRELRGADLENAKLGSVKLSGADLSALPMEKRTKRTNLESANLRGADLTN
                     ANLANAAASGAYLREADLTGADLSGADLSSANLKDANLTDAKLDGAKLDGAKFCGTVM
                     PDGSKNDEGCAP"
     gene            1580..1933
                     /gene="arsC"
                     /locus_tag="AC16Y7_00290"
     CDS             1580..1933
                     /gene="arsC"
                     /locus_tag="AC16Y7_00290"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995352.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HIR5707848.1"
                     /translation="MPRTATIYHNPRCSKSRQTLALLEENGVEPKIVEYLKTPPSAAE
                     LKRVLKRLGLSPHDIVRKGEAVYAELGLKDKDVSDEELIKLMVVNPILIERPIVVSGG
                     KAAIGRPPEAVLKIV"
     gene            complement(1930..2250)
                     /locus_tag="AC16Y7_00295"
     CDS             complement(1930..2250)
                     /locus_tag="AC16Y7_00295"
                     /inference="COORDINATES: protein motif:HMM:NF039461.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6460 domain-containing protein"
                     /protein_id="HIR5707849.1"
                     /translation="MERFFGGNPGLVLLRLVILSLIVGVVLAALGFSPYDILQNLQDL
                     AARIYDMGFAAVEKAFRYFLLGAVIVFPVWIVMRMFKVLGRDTDTQVPDTQVAETPAP
                     NRET"
     gene            2352..4910
                     /locus_tag="AC16Y7_00300"
     CDS             2352..4910
                     /locus_tag="AC16Y7_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HIR5707850.1"
                     /translation="MGVSARKELKTAAADPAASDARLPEPFAGWFAARGWVPRAHQLA
                     LLDTVSAGRDTLLIAPTGGGKTLAGFLPSLIDLAKRPLTRAPATSVHTLYVSPLKALA
                     TDIARNLTAPVEEMGLSIGLETRTGDTPQARRARQRVLPPDILLTTPEQIALMLAHRS
                     AAELFGSLKCVILDELHALATSKRGDLLALDLARLRNLSPELMAIGLSATVARPSELA
                     AFLVPHRDQAATRLAEIVTAEGGAHADITILKSNADLPWAGHSARYALPEIYESIKAH
                     NMSLMFVNTRSQAEMLFHELWRCNDDNLAIALHHGSLDVGQRRKVEAAMVAGKLRAVV
                     CTSTLDLGIDWGDVDLVVNVGAPKGASRLAQRIGRANHRLDEPSKALLVPANRFEVME
                     CQAALEAAEEGAQDTPPTRPGALDVLAQHILGMACAEPFDADGLYREVTSASPYQVLT
                     REGFDQVLAFVATGGYALANYDRYAKLKRTKEGTYRVAHPRIAQQYRLNVGTIVEAPM
                     LRVRLARGALKKSGAVVGGRVLGEVEEYFIEQLSPGDTFAFAGEVLRFEGLREMDAIV
                     TRAHDSDPKVPAYEGGKFPLSTYLADRVRHMLAERGDWSKLPGQVSDWLRLQAEFSVV
                     PSADEVLIETFPRGQRHYLVCYPFEGRLAHQSLGMLLTRRLERAGAAPLGFVANEYAL
                     AVWGLNDMSEMIAAKHLDLAALFDEDMLGDDLDAWLAESNLMKRSFRVCAIISGLIER
                     RHPGQEKTGRQMTVSSDLIYDVLRQHEPDHVLLKAAFADAAAGLIDLGRLGQFLKRIG
                     GRIRHSEVSHVSPLAVPVMLEIGRESVPGDIGDTLLRETAEELIAEALGEHAAR"
     gene            4897..5622
                     /gene="pdeM"
                     /locus_tag="AC16Y7_00305"
     CDS             4897..5622
                     /gene="pdeM"
                     /locus_tag="AC16Y7_00305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020088000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HIR5707851.1"
                     /translation="MLRARLDLMPETAPTLRSHLSVSGHDFQPLVAGALYWEAEDTLL
                     VADLHMEKGAAFAALGMLLPPYDTRSTLARLRKIIKRIDPGRVVSLGDSFHRSECADH
                     MVADDLALLTKLQKGREWYWICGNHDPHLPECVGGTVCETLTISGVTLRHEPSETNSG
                     PEIVGHLHPVARIARRGMVVRRRCFATDGNRLVMPAFGAYAGGLNVLHGAFQPLFRWH
                     QVEAWMMGRRAVYPVLGSLLLPD"
     gene            complement(5626..6432)
                     /locus_tag="AC16Y7_00310"
     CDS             complement(5626..6432)
                     /locus_tag="AC16Y7_00310"
                     /inference="COORDINATES: protein motif:HMM:NF021148.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HIR5707852.1"
                     /translation="MRAATLFLALLLTALIGAPGFAQQTPPLPAEKPESKETVQSDIS
                     TREIAIQSNFTGIEILIFGSVDFSDSRTPGSGTYDVIAVVRGPDEPIVVRKKQRVAGI
                     WVNGPGKTYDNVPGFYAVLSSRPFRAIASDERLKELGIGLSELDFGRHIPSESQEQAF
                     RTAMIRLMEEKELFKEDDDGVTFIGRSLFRATVALPVSVPTGRYLVDVYLFRDGELAS
                     ESQGTLEVTKAGIEAFLYTLAFQHPFVYGLLGVLIAVLMGLIGWYAFRRE"
     gene            complement(6435..7361)
                     /locus_tag="AC16Y7_00315"
     CDS             complement(6435..7361)
                     /locus_tag="AC16Y7_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011974359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR5707853.1"
                     /translation="MFQIYLPIAEISVNLLVMLGLGATVGFLAGMFGVGGGFLLTPFL
                     MLSGIPPAIAVATGANQIVGASVSGTLAQWRRGNVDVQMGGILIAGGIAGAVVGVLLL
                     KYLREAGQAGLIISLTYVCFLGSIGALMLWESINAIRRARAGKPVSARKSGQHIWIHG
                     LPLKMRFPRSRLYISIIPPLVIGVLVGLLASFLGVGGGFIMVPAMIYLLRMPTNIVIG
                     TSIFQIIFVTAIVTLLHATLNHTLDIVLAFLLIVGGVVGGQFGVRAGQKLRGEQLRAL
                     LALMVLAVALRLLFDLVARPSNLYSIVPLAGA"
     gene            complement(7500..8393)
                     /locus_tag="AC16Y7_00320"
     CDS             complement(7500..8393)
                     /locus_tag="AC16Y7_00320"
                     /inference="COORDINATES: protein motif:HMM:NF013625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HIR5707854.1"
                     /translation="MRSRTPRQRRRRCRRLHSARSHCARPASEGDAAAQYEIAVRYAE
                     GKGTKRDAKMAAQWFERAGRDGLAPAQYRLAAMYERGLGVDRDPVKARAWYKAAAQNG
                     NVKAMHNLAVSVSGRDGGAPDYTFAAKWYGQAAAHGLPDSQFNLGIVAEHGLGRTKSL
                     GEAYKWYALAAATGDTESTKRRDVVAVQLTPETRAKAEAEVKAWKATPALPPANQVAE
                     NPAWTAKKTASTAKASNASTLVTRAQTLLNKLGYDVGVPDGVAGSRTRSEIKRFQGRN
                     GLEETGEVSVPPVSQLESLAS"
     gene            complement(8308..>9926)
                     /locus_tag="AC16Y7_00325"
     CDS             complement(8308..>9926)
                     /locus_tag="AC16Y7_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707855.1"
                     /translation="IAANAQSALALDTDPYASDVTPAVDHTGLDKRLVDMAERLERSI
                     GDAMPASAIQSLNARLDEIGDQLSRSLDTAPSRQALEHVERQISDMGQQLNRAEEQLG
                     KFGGVEEHLLKLIERLDQKDAEGSKPIDPLQLQEVAAKAATDAARIVADDSKKTTERL
                     DALQRDLSDMSDQSRQSGDKLVSTLEAVHESLKQLVQQVERGNSFVPGQRSPFTERAP
                     QADAPPAAPFASQAAPQAPAPQAATPSPQGSEAPQPSPAPRMPEMKPRMPAAPAPNET
                     PDVAVKDQQLRDRLDAAMPGLNETETPPPFGRSKPSPLDQNAFDLDAKPAQPRQSPDD
                     LVAAARRAAQAAAARAEERDGRRSKMPLPGSGTAEPGRRKRSWLMIAAATLLAVSAIL
                     LYGRLGSKPEPEPTPAATGQTAPASPDAGTEAGDTAPASKAKPADELTPAEPDQSGFW
                     APPPGTTIEPIDLGQAATGTTDIAKGRMPAMPASELSASPQLASLKPTNGPALPPDVV
                     FSVEEPDASAAKAPMPPAALGPLPLRKARL"
BASE COUNT         1720 a         3177 c         3235 g         1794 t
ORIGIN      
        1 aagcaggccg tattcggcct cgactttccc aatcccatcg gcatggcgcc cggcttcgac
       61 aaggatgcgc gcgtaccgca ccagctcttg gagatggggt tcggtttcgt cgaggtgggt
      121 acgctcacgc cgcgtgcgca atccggcaat ccggccccgc gcttgttccg ctcccatcgg
      181 gaccgcgcca tcgtcaatcg cctcggcttc aacaatgaag gccaggacgc ggcgctgaca
      241 cgtctggcga ggcgcccgcg cggaatcgtc ggcgtcaatc tcggcgccaa ccgcgacagc
      301 accgaccgca tcgccgatta cgccgacggc atcacgcgca tggcacccgt ggcggactat
      361 ttcacgatca atatctcctc gcccaacacg ccgggcctgc gcgacctgca agcaccgcgc
      421 aagctcgatg cgctgctcgg taaagtgcag gaggcacgca aagcgcttgg gccgaaagcc
      481 ccgccgctcc tggtgaagct cgcgccggat ctcgccgatg ccgatttgcc ggaagtggcc
      541 gccgtgatcg aacagaatga ggccgacggg attatcgttt ccaacaccac gctggcgcgg
      601 gataatttgc gcgatgaaac gcttctccgc gaggcgggcg gcatgtccgg gcgtcccgcg
      661 ttcctacgta cgacccgcat gctggcgcgc gtccatcaag ccaccgaagg acggctgccg
      721 ctgattggcg tcggcgggct cgattccggc gaggcggcgc tggccaagat cgaagccggt
      781 gcctcgctga tccagcttta caccgcgctc gtcttcgaag ggcccggcct gattgggcgc
      841 atcaaggaca cgcttgtgcg ggccatggcg cgcgagaatg ccgacaacct caagcccttg
      901 atcgggcgcc gcgctggcga gtggtcggag aaggaactgc cggacgtcgt tgtctgacct
      961 tgcccgccgc tgttacggcg cacacccttc gtcgttcttt gagccgtcgg gcataacagt
     1021 tccgcagaac ttcgcaccat cgagttttgc gccatccaac ttggcgtcgg tcaaattggc
     1081 gtccttcaga ttggcgctcg acagatcggc ccccgaaaga tcggcgccag tgagatcggc
     1141 ttcgcgcaga taagcacctg aagctgcggc gttcgcaagg ttggcattgg tgaggtctgc
     1201 gccgcgtaag ttggcgcttt cgaggttcgt ccgcttggtg cgcttttcca ttggcaatgc
     1261 agagaggtcc gcgccgctca acttaacgct gcccagcttg gcgttttcca gatccgcgcc
     1321 gcgcaactcc cgcccgctca ggtcggcttc gctgaggtcg caatatttgc aagcattggt
     1381 ggtcagaaac gtatcgagct tcgccttgtc gaaggcgagg ctgagacttg cgtccgctgc
     1441 aactgaagct agaaagacga ctgtgcaaag aaaataggtc ttgggtttca tttttgccct
     1501 ctccgtgagt ccggcagcct agcaacgttc acggcaatgt tcattctcaa gccggctatt
     1561 tctggcttac cttcgtaaaa tgcctagaac agccaccatc taccacaatc cgcgctgctc
     1621 caagtcgcgc cagacgctcg cgctgttgga ggaaaacggc gttgagccga aaattgtcga
     1681 atatctgaag acgccgccct ccgctgccga actcaagcgc gtgttgaaga gactcgggtt
     1741 gagcccccac gacatcgtgc gcaaaggcga ggccgtctat gccgagcttg gcctgaagga
     1801 caaagacgtc tccgatgaag agctgatcaa gctcatggtc gttaacccaa tcctgatcga
     1861 gcggcccatc gtcgtctcgg gcggtaaggc ggccattggc cgcccgccgg aagccgtctt
     1921 gaaaatcgtc taggtctcgc ggttcggcgc aggcgtctca gcgacctgcg tatcgggaac
     1981 ttgcgtgtcg gtgtcccgcc ccagaacctt gaacatgcgc atcacgatcc agacggggaa
     2041 gacgatcacg gcacccagca agaagtagcg gaaggccttc tccaccgcgg caaagcccat
     2101 atcgtagatg cgggccgcca ggtcctgcag gttctggagg atgtcgtagg ggctgaagcc
     2161 cagcgccgcg aggacgacgc ccacgatcag ggataagatg accagccgga gcagaaccag
     2221 ccccggattg ccgccaaaaa accgttccat tttcgcttcc cttgaatcgt gagacgtttg
     2281 cattgggtga gccccacaat tggggctcca aacggcgaag gtcaaggcga ggcggcaacc
     2341 ggaaagggct gatgggagtg tccgcgcgga aggagctgaa gaccgcagcc gccgatccgg
     2401 cggcaagcga cgcgcggctg cctgagccct tcgccggctg gtttgccgcc cgcggctggg
     2461 tcccgcgcgc gcaccaattg gcgctgctcg ataccgtatc cgcggggcgc gatacgctgc
     2521 tgatcgcgcc gaccggcggc ggcaagacgc ttgccggctt cttgccgagc ctcattgatc
     2581 ttgcgaagcg tccgctcacc cgcgcgccgg cgaccagcgt gcacacgctt tatgtctcgc
     2641 cactgaaagc acttgccacc gacatcgcgc ggaatctgac ggcgccggtc gaagagatgg
     2701 ggctctccat cgggctggag acccgcaccg gcgacacgcc gcaagcccgg cgggcacgcc
     2761 agcgcgtcct gccgcccgac attctcctga cgacgccgga gcagatcgcg ctgatgcttg
     2821 cgcacagaag cgccgccgaa ctgttcggct cgctcaaatg cgtgatcctc gatgaattgc
     2881 atgcgcttgc caccagtaag cgcggcgacc ttctggcgct cgaccttgcg cggctacgca
     2941 acctgtcgcc tgagcttatg gcaatcggtc tgtcggcgac cgtcgcgcgc ccctccgagc
     3001 tcgccgcatt cctggtgccg cacagggacc aggccgcgac gcgccttgcc gagatcgtca
     3061 ccgccgaggg cggcgcccac gcggacatca ccattctcaa gtcgaacgcg gatctgcctt
     3121 gggcgggaca ttcggcgcgt tacgcattgc cggagatcta cgaaagcatc aaggcgcaca
     3181 acatgtcgct gatgttcgtg aacacgcgtt cgcaagccga gatgctgttc cacgagctgt
     3241 ggcgctgcaa tgacgacaat ctcgcaatcg cgctccacca cggctcgctc gatgtgggtc
     3301 agcggcgcaa agtggaggcg gccatggtgg ccggcaagct ccgcgccgtg gtctgcacct
     3361 cgacgcttga tctcggtatc gactggggcg atgtcgatct cgtcgttaat gtgggcgcgc
     3421 cgaaaggcgc aagccggctc gcgcagcgta ttggccgcgc caatcatagg ctcgacgagc
     3481 cgagcaaggc gctgctcgta ccggccaatc gcttcgaggt gatggaatgt caggcggcgc
     3541 tcgaggccgc cgaagaaggc gcgcaggata ccccgcccac gcggcccggc gcgctcgacg
     3601 tgctggcgca gcacattctc ggcatggctt gcgcggaacc tttcgacgcc gacgggcttt
     3661 atcgtgaagt gaccagcgca tccccttatc aagtcctcac ccgcgagggc ttcgaccagg
     3721 ttctcgcctt cgtcgccacg ggtggttacg cgctcgccaa ttacgatcgc tacgccaagc
     3781 tgaaacgcac caaggaaggc acgtaccgcg tggcccatcc gcgcatcgcg caacaatacc
     3841 ggctcaatgt cggcaccatt gtcgaagcgc ccatgttgcg cgtgcggctc gcccgcggcg
     3901 cgctcaagaa atcgggtgca gtagtcggcg ggcgcgtcct cggcgaagtc gaggaatatt
     3961 tcatcgagca gctctcaccc ggcgacacct tcgcctttgc cggcgaggtg ctacgcttcg
     4021 aaggcttgcg cgagatggat gccatcgtca cccgcgcgca tgattccgac ccgaaagtgc
     4081 cggcttacga aggtggcaag ttcccgctgt ccacctatct tgccgatcgc gtccggcaca
     4141 tgctggccga acgcggagac tggtccaaac taccgggcca agtttccgat tggctgagac
     4201 ttcaggcaga gttctcagtc gtaccttccg ccgacgaggt gctgatcgag acgtttccaa
     4261 gagggcagcg tcactatctc gtttgctacc cgtttgaagg gcgtctcgct catcagagtc
     4321 tcggcatgct gctgacaagg cgcctggagc gcgcaggcgc ggccccgctc ggcttcgtcg
     4381 ccaacgagta tgcgctggcg gtctgggggc tcaacgatat gagcgagatg atcgcggcaa
     4441 agcatctcga cctcgcggca ctcttcgacg aggacatgtt gggcgacgac ctcgatgcgt
     4501 ggctggcgga gtccaacctg atgaagcggt ccttccgcgt ctgcgccatc atctccggcc
     4561 ttatcgagcg gcgtcatccc ggacaggaaa agaccgggcg gcagatgacc gtctcctccg
     4621 atctcatcta cgatgtgctg cgccagcacg agccggacca cgttttgctg aaagcggcat
     4681 tcgccgacgc cgcggcgggg ctgatcgatc ttggccgctt gggacaattc ctgaagcgga
     4741 tcggcggtcg aatcaggcat agtgaggtga gtcacgtttc gccgctcgcc gtgcccgtca
     4801 tgcttgagat cggccgcgaa agcgtcccgg gcgacattgg cgacaccttg ttgcgtgaga
     4861 ccgccgaaga actgatcgca gaagcattgg gggaacatgc tgcgcgctag gctcgatctc
     4921 atgccggaaa cggcgccgac cttgcgctcg cacctgtcgg tgtcgggcca cgacttccag
     4981 ccgctcgttg cgggcgcgct ctattgggaa gcggaagaca cgcttctcgt cgccgacctg
     5041 cacatggaga agggggccgc gttcgccgcg ctcggcatgc tgctcccgcc atacgacaca
     5101 cgctccacgc tggcgcgcct gcgcaagatc atcaagcgga tcgatccggg ccgcgtggta
     5161 tcgctcggcg acagcttcca ccgcagcgaa tgcgccgacc atatggtcgc cgacgatctg
     5221 gcgcttctca ccaagcttca aaagggtcgc gagtggtact ggatctgcgg taatcacgat
     5281 ccgcatttgc cggaatgcgt cggcggcacg gtctgcgaga cgctcaccat tagcggcgtg
     5341 acgctgcgcc acgaaccgtc cgaaaccaat tcgggtccgg agattgtcgg acatctgcat
     5401 cccgtggcgc gcattgcccg ccggggcatg gtggtccggc ggcgctgttt cgccaccgac
     5461 ggcaaccgcc tggtgatgcc ggccttcggt gcttatgcgg gcggtctcaa cgtgctgcac
     5521 ggcgccttcc agccgctatt ccgctggcat caagttgagg cgtggatgat gggccgccgc
     5581 gcggtctatc ccgtgctggg cagcctgctt ctgcctgatt agccgctact cgcggcggaa
     5641 ggcataccag ccgatcagac ccatcaatac ggcgataagc acgccgagca ggccgtagac
     5701 gaagggatgc tggaaggcga gcgtgtagag aaacgcttcg atccccgcct ttgtcacttc
     5761 cagcgtgcct tggctctcgc tcgccaattc tccgtcgcgg aacagataga cgtcgaccag
     5821 atagcgtccc gtcggcacgc tgaccggcaa cgccaccgtg gcgcggaaca ggctccggcc
     5881 aataaaagtc acgccgtcgt cgtcctcttt gaagagttcc ttctcctcca tcagccggat
     5941 catggccgtg cggaaggctt gctcctggga ctcgctgggg atgtgccggc cgaagtcgag
     6001 ttcagacaga ccgatgccca gctccttcaa cctctcgtcg gaggcgatgg cgcggaacgg
     6061 gcgcgaggac agcacggcgt agaagcccgg tacgttgtcg taggtcttgc cgggaccgtt
     6121 gacccagatg cctgcgacac gctgcttctt ccgaacgacg atcggctcgt cgggcccgcg
     6181 caccacggcg ataacgtcat acgtgccgct tcccggtgtt cgagagtcac taaagtcgac
     6241 gctgccgaaa ataagtattt cgatgccggt gaagttcgac tggatcgcga tctcgcgcgt
     6301 cgagatgtcg gactggacgg tttccttgct ctccggcttt tcggccggca gtggcggcgt
     6361 ctgctgcgca aaccctggcg caccgatcag tgcggtcaaa agaagggcaa ggaagagggt
     6421 tgctgctctc atcatcatgc tcccgccaac ggaacgatgc tgtagaggtt cgacgggcgc
     6481 gccaccaggt caaacaagag gcgcaaggcg acggcgagga ccatcagcgc gagcagcgcg
     6541 cgcaattgct cgccgcgcag tttctgtccg gcccgcacgc cgaactgtcc gccgacgacg
     6601 ccgccaacga tgagcaagaa ggccagcacg atgtcgagcg tgtgattgag cgtcgcgtgc
     6661 aggagcgtga cgatggcggt cacgaagatg atctgaaaga tcgatgtgcc gatcacgata
     6721 ttcgtcggca tgcgcaagag atagatcatg gccgggacca tgatgaagcc gccgccgacg
     6781 ccgaggaacg aggcgagcag tccgaccagg acgccgatga cgagcggggg gatgatgctg
     6841 atatagagac gcgaacgcgg aaagcgcatc ttcagcggca ggccgtgaat ccagatgtgc
     6901 tgtccggatt tgcgtgccga gaccggcttg ccggcccgcg cccgcctgat ggcgttgatg
     6961 ctctcccaca gcatcagcgc gccaatgctt ccgaggaagc acacataggt cagcgagatg
     7021 atcaggccgg cttggcccgc ttcgcgcaga tatttcagca gcagcacgcc gaccaccgcg
     7081 cccgcaatac cgccggcaat caggatgccg cccatctgga cgtcgacatt gccgcgccgc
     7141 cattgggcga gggtgccgga caccgaggct ccgacaatct ggttggcgcc ggtggccacg
     7201 gcgatcgccg ggggaatgcc ggaaagcatc agaaatggcg tgagcaggaa gccgccgccg
     7261 accccgaaca tgcccgccag gaagcccacc gtcgcgccaa gccccagcat gaccaaaagg
     7321 ttcaccgaga tctcagcaat gggcagataa atctgaaaca tcggccaggc gttgggaaaa
     7381 gcgggcgctc aggcaaactg ttcgccgcgc gcgggcctgt caatgcataa gccgcgaact
     7441 taccgaaatg tcaccgaaat tcgtcgccag gcggggaaat gcccgcttct ggctgccggc
     7501 tagctggcca ggctctcgag ctggctgacg ggcggcacgg aaacctcgcc cgtctcttcc
     7561 aggccgttgc ggccctggaa ccgtttgatc tcggaccggg tgcggctgcc ggcgacgccg
     7621 tccggcacgc cgacgtcata gccgagcttg ttgagcaggg tctgggcccg agtcaccagc
     7681 gtggaggcgt tggaagcctt cgccgtcgat gccgtcttct ttgccgtcca ggccgggttc
     7741 tccgcgacct ggttggcggg aggcagggca ggcgtggcct tccaggcctt cacttccgcc
     7801 tcggccttgg cgcgcgtctc cggggtcagc tgcacggcaa cgacgtcgcg ccgcttggtg
     7861 gattcggtat cgcctgtggc ggcggccagt gcgtaccact tataggcctc gccgaggctc
     7921 ttggtcctgc cgagcccgtg ctcggcgacg atgccgagat tgaactggct gtccggaagg
     7981 ccatgcgcgg cagcttggcc gtaccatttg gcggcaaagg tatagtccgg cgcgccgccg
     8041 tcgcggccgc tgacgctcac cgcgagattg tgcatggctt tgacattgcc gttctgggca
     8101 gccgctttgt accaggcgcg cgcctttacg gggtcacgat caacgcccag gccgcgctca
     8161 tacatggccg cgagccggta ttgggccggg gcgaggccgt cgcggcctgc gcgctcgaac
     8221 cactgggcgg ccatcttggc atcgcgcttg gtgcctttgc cttcggcgta acgcacggcg
     8281 atttcgtatt gcgcggcggc atcgccctca gaggcgggcc ttgcgcagtg ggagcgggcc
     8341 gagtgcagcc ggcggcatcg gcgccttcgc tgccgaggcg tccggctcct caacagagaa
     8401 gacgacgtcc ggcggaagag ccggcccatt cgtgggtttc agcgaggcga gctgcggcga
     8461 tgcggagagc tcgctcgccg gcatggcggg catgcgtcct ttggctatgt ccgtcgtgcc
     8521 cgttgccgcc tgcccgaggt cgatcggttc gatcgtggtg ccgggaggtg gcgcccagaa
     8581 gccggactga tcgggctccg caggcgtaag ctcgtcggcc ggtttggctt tgcttgccgg
     8641 agcggtgtcg ccggcctcgg tgcctgcatc cgggctggcc ggtgcggtct gtccggtggc
     8701 ggccggggtc ggctccggct ccggcttgga accgagacgg ccataaagaa ggatggcgct
     8761 caccgcgaga agtgtggcgg cggcgatcat caaccaggaa cgcttgcggc gccccggttc
     8821 tgcagttccg ctgccgggaa gcggcatctt ggaccggcgt ccgtcgcgct cctcggcccg
     8881 agccgccgct gcctgcgccg cacggcgcgc cgcggcaacg aggtcgtccg gcgactggcg
     8941 gggctgcgcc ggcttggcat ccaggtcgaa agcgttttga tccaaaggcg acggcttgga
     9001 gcgcccgaac gggggcggcg tttcggtctc gttgaggccc ggcatcgccg cgtccaacct
     9061 gtcgcggagc tgctgatcct tgactgccac atcgggcgtc tcgttcgggg ccggcgcagc
     9121 cggcatgcgc ggcttcattt ccggcatgcg cggcgcgggg ctaggctgcg gcgcttcaga
     9181 accttgcggg cttggggtag ctgcctgcgg tgcgggggct tgcggcgcag cttgcgatgc
     9241 aaacggagcg gcaggcgggg cgtcggcttg cggtgcgcgc tccgtgaatg gtgagcgctg
     9301 ccccggcacg aaggaattgc cacgctcgac ctgctgaacg agctgtttga gggattcgtg
     9361 gaccgcctcg agcgtgctga ccagcttgtc gcccgattgg cggctctggt cgctcatgtc
     9421 cgagaggtcg cgttgcagcg cgtcgagccg ctcggtggtc ttcttgctgt cgtcggcgac
     9481 gatgcgtgca gcatccgtcg cagccttggc ggcgacctcc tgaagctgta gcggatcgat
     9541 cggctttgag ccttcagcgt ccttttgatc cagacgctcg atcagcttga gaagatgctc
     9601 ctccaccccg ccgaacttgc cgagctgttc ctcggcgcgg ttaagctgtt ggcccatatc
     9661 ggaaatttgg cgttcgacat gctccagagc ctggcggctg ggggccgtat ccaaggaccg
     9721 ggagagctgg tctccgatct cgtcgagcct ggcgttcaag ctctgaatgg cgctcgccgg
     9781 catggcgtcg ccgatcgacc gctcgagccg ctcggccatg tccacgaggc gcttgtcgag
     9841 gccggtgtga tcgacggccg gcgttacatc gctggcgtaa gggtcggtgt ccagggccag
     9901 ggcagactgc gcatttgcag cgatgg
//
LOCUS       DBNHVU010000008         2890 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1417756, whole genome shotgun sequence.
ACCESSION   DBNHVU010000008 DBNHVU010000000
VERSION     DBNHVU010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1417756"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="AC16Y7_00330"
     CDS             complement(<1..991)
                     /locus_tag="AC16Y7_00330"
                     /inference="COORDINATES: protein motif:HMM:NF026760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-hydrolase family protein"
                     /protein_id="HIR5707856.1"
                     /translation="MAFFIGYAIGYGCVTLWRYLELPEVPRSWWRAANIALAVACAIA
                     AAIYIPQAAAWQDEVRARLNMPPTETANLLQVIFVAAIFALLLMLLARLLIGGTRATS
                     SLVYRWMPRRIAALLGAVIFAVLLVTLVNGTLVSAIIGAIDRAQALVDVTNPPGASVP
                     REATRSGGPGSQIAWQDLGRAGKRFVTEGPRKADIEEFSRRAALEPIRVYAGLRSADS
                     PEEQAELALADLIQMGAFSRSLLVIVTPTGTGWVDNNGIAPLEYMHDGDVATVAVQYS
                     YLQSPLSLILEPGRSQASAAAVFRAIYNYWSKLPEANRPKLYLFGLSLGSLGSE"
     gene            complement(1486..1710)
                     /locus_tag="AC16Y7_00335"
     CDS             complement(1486..1710)
                     /locus_tag="AC16Y7_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707857.1"
                     /translation="MRYRSIVFASLALASALVLTALAPQPGSAANMSPAQLDQIQSTR
                     IVTPAHCRAVRHCHRRCYRGRCTRYCHRCG"
     gene            1898..2596
                     /locus_tag="AC16Y7_00340"
     CDS             1898..2596
                     /locus_tag="AC16Y7_00340"
                     /inference="COORDINATES: protein motif:HMM:NF016206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HIR5707858.1"
                     /translation="MFEDPLFWLLVGPGLALGLYAQARIKANVTKYSQVPTRDGITGA
                     EVARRLLDAKGLQDVKVETTEGVLSDHYDPRAKVLRLSQEVYYTPSVAAAGIAAHETG
                     HALQDARDYFPMELRTYIVPVVQLASQAAPWAFVGGLMLQWDVLTWIGVILFGASFVF
                     ALLTLPVEFNASARAKELLMSHGIIGKKELEGVDKVLDAAAWTYVAAAVSAIGVWLFY
                     VFMLIGRGRSASAR"
BASE COUNT          580 a          880 c          936 g          494 t
ORIGIN      
        1 cttccgatcc gagcgaaccg aggctgagac caaacagata gagtttcggg cggttggcct
       61 ccggaagttt gctccaatag ttgtaaatgg ccctgaacac ggcggctgca gacgcctgcg
      121 agcgtccggg ctcaaggatc aacgacaggg ggctttggag ataggagtac tgaacggcga
      181 ctgtcgcgac atctccgtca tgcatgtatt ccagtggcgc gatgccatta ttgtcgaccc
      241 accccgtgcc tgtgggcgtc acgatgacca acaatgaccg ggagaaagcg cccatttgga
      301 ttagatcggc gagggccaac tcagcctgct cctcgggcga atccgccgag cggagccctg
      361 catagacgcg gatcggctca agtgctgcgc gacggctgaa ttcctcgata tctgccttcc
      421 tcggtccttc tgtaacgaac cgcttgccgg ctcgaccgag atcctgccac gcgatttgag
      481 atccgggacc acctgatctc gtggcctccc ttggtactga tgcacccggc ggattggtga
      541 catccaccag cgcttgagcg cggtcaattg cgcctatgat ggcgctaacc agcgtgccat
      601 tcaccaacgt caccaggagc accgcgaaga tcacggcacc gagcagcgca gcgatcctcc
      661 tcggcatcca acgatagacg agggatgacg tagctcgggt ccccccgatc aacaagcgcg
      721 cgagaagcat cagaagcagc gcaaagatgg ccgcgacaaa gatcacttgc aggaggtttg
      781 cggtttccgt cggcggcata ttcaaacggg cgcggacttc gtcctgccac gcggcggctt
      841 gcggaatata gatggcggca gcaatcgcgc aggccactgc cagagcgatg ttcgctgccc
      901 gccaccatga ccgcggcact tccggtagct cgagatagcg ccacaatgtc acgcagccgt
      961 acccgatggc atagccaatg aagaaggcca agccgccgag taggccctga aaaatcggcg
     1021 gccgtggaac caaagaagga gtgaaggtaa ggacgaaaaa cagcagaccg agaaaaaccc
     1081 cggtcaagga cggcagccac caagggggcg cctgagtttc tttggtagag cctatccttt
     1141 cgatcatgct ttggagcttt tcgaagctgg acaactatgg caagagccca gaagcgattg
     1201 actgacgcgc cgggcgacca agtcggcttc tggcgccgag ccaaaaactt atccccggac
     1261 ctcgccacac ccccataatc cgcccatccc tcgttcatga gggcgcgagc tgcagaccgt
     1321 ctgctcaagt agggtcgccc tcaactaggt ccaaggccgg tagggcaaac agaagaggcc
     1381 ggtcgcgcaa acaaaacccc ggggccggcg acgaacatgc gtgactgctc cctctccctg
     1441 aaagggggag ggctgtggtg ggagtcaatg ggtgactctc cgctcctaac cgcagcggtg
     1501 gcaatagcgc gtgcagcggc cgcggtagca gcgccggtgg caatggcgca ccgcgcggca
     1561 atgggccggc gtcacgatcc ttgtggactg gatctggtcc agctgcgccg ggctcatatt
     1621 ggcggcgctg cccggttgcg gggcgagcgc ggtgagcaca agggcgctag cgagggcgag
     1681 actggcgaag acgatcgatc tatagcgcat acgatcctcc cgttattggt gcccgcgcgt
     1741 cgaaagcggc gcgggccttg acgccagatt atcacgccga tcggcgtgcg ctcgccttga
     1801 tcccgctcaa atgcgctcaa gttggaccgc atgccgactt gggatggcgt gctagactgg
     1861 ccagcaatgt tggacgcgcg gaaggatcgg agcaacgatg tttgaagatc cgctgttttg
     1921 gctgctggtc gggccgggcc tggcgctcgg gctttatgcg caagcccgca tcaaggcgaa
     1981 cgtcaccaaa tattcgcaag tgccgacgcg ggacggcatc acgggcgcgg aagtggcgcg
     2041 gcggcttttg gatgcgaagg gactgcagga cgtcaaggtg gagaccaccg aaggcgtgct
     2101 gtccgatcac tacgacccgc gcgccaaggt cttgcgcctc agccaggagg tctattacac
     2161 gccgtccgtc gccgccgccg gcatcgccgc ccatgagacg ggccacgcgc tccaggatgc
     2221 gcgggactat ttccccatgg agctgcgcac ctacatcgtg ccggtggtgc agctcgcctc
     2281 gcaagccgcg ccttgggcct ttgtcggtgg gctgatgctg caatgggacg tgctgacctg
     2341 gattggcgtc atcctgttcg gcgcctcctt cgtcttcgcg ctgttgacct tgcccgtgga
     2401 gttcaacgcc tccgcccgcg ccaaggagct gctcatgagc cacggcatta tcggcaagaa
     2461 ggagctggag ggcgtcgaca aggtgctgga cgcggcggcc tggacctatg tggcggcggc
     2521 ggtctcggcc atcggcgtct ggctgttcta cgtgttcatg ctgatcggcc gcggccgcag
     2581 cgcgtcggcg cgctaaaacg cgctcaagta gcgcgaagcg cggtagcgca tagaagaagc
     2641 gcggtagcgc aaagaagatg acgaagacgt gagaggccac gccccgatcc cgccataggc
     2701 gagggtgata gagtgggctt aaggagccca gatcatgacc gataccgcaa tacccaccga
     2761 cgacgcctcg ggcgcgatcg cactcgacga ggcctgcgtc tccggcgaaa tcgccgatgc
     2821 gatcatcgct tcgggcctga ccgaccggcc cgtgggcgat ctcgtggaga cggtcatggg
     2881 gcggctggtg
//
LOCUS       DBNHVU010000009         6039 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_897572, whole genome shotgun sequence.
ACCESSION   DBNHVU010000009 DBNHVU010000000
VERSION     DBNHVU010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6039)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6039
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_897572"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..2922
                     /locus_tag="AC16Y7_00345"
     CDS             253..2922
                     /locus_tag="AC16Y7_00345"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HIR5707859.1"
                     /translation="MDITTPVTPPKRGRKKKRRGFFLRFLGFMFAAGMILAIGVAGAV
                     AFVLWKASQDLPDYEVLAKYEPPVMTRIHANDGRLISEFARQRRIYVPITAVPKRVIE
                     AFLSAEDKNFYQHGGLDIQGIIRAIVTNMSAMQAGGGHMVGASTITQQVAKNFLLSSE
                     QNIERKLKEAILAIRIERAFTKDQILELYLNEIYLGGGAYGVAAAAQRYWGKALNELS
                     WAEAAYLALLPKAPSRYDPWKHAERALTRRNWVIDRIVENGFATPEEGEQAKATPLGV
                     LAKKYGPQIHASEYFAEEVRREILDKFGEDKLYGGGLSVRTTLDPRLQAIARKALVKG
                     LTKYDRAHAGWRGVDKTIEISGDWGKALAEIPVWKDIAPWKRAVVLEVSGDQATIGIR
                     PGRDKEGRLVEERETGVIPASQVKWTRKSIKSALKPGDVIYVAPLEPKKNKDGEVAPA
                     ETVAGKWSLQQPPKVSGAITAIDPHTGRVLAIVGGFSFHQSQFNRATQALRQPGSSFK
                     PLIYLTAIDNGYTPSSIIVDGRICVSQGRGMPPWCPKNYSGGGAGPSTLRRGIEKSRN
                     LMTVRLSRDIGMPVIAEYSNRFGVYDNLVPALAMSLGSGETTLLRMVNAYSMIANGGK
                     KVEATFIDRVQDRYGRTVWRHDKRDCSLCSAREWTGQGEPELIDVRDQVVDPVSAYQM
                     VGIMEGVVQRGTATRLRALKRPLAGKTGTTNDYKDAWFVGYTPDMAVGVYVGYDQPKP
                     MGRSATGGGLAAPIVKDFLAEALKDVPPAEFRPPEGAVLVRVNSKTGLPARGGGGGTI
                     VEAFKPNEVPPGAIPLGRDGKPQGQAAEEGAQPDGFPQYPAEAQAGAPGQPAGAQAAA
                     PQAAPQPPPQASAPPPRQRRAPRRERGLFRGFF"
     gene            3008..4136
                     /gene="prfB"
                     /locus_tag="AC16Y7_00350"
     CDS             join(3008..3076,3078..4136)
                     /gene="prfB"
                     /locus_tag="AC16Y7_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_143006121.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR5707860.1"
                     /translation="MRAETEAVVNDIKESLSLLRRHLDPDQAKARLSELDREAEAPDF
                     WNDAGRAQTLMRERQQIETSLNAFDRLANELDDNLTLVSMGEEEGDDSIVAEAEAALQ
                     KLRAEAHTREVEALLSGEADANDAFIEIHAGAGGTESQDWAQMLERMYLRWSEARGYK
                     TELIGEHYGDEAGIKSATILVKGLNAYGWLKTESGVHRLVRISPFDSNARRHTSFASV
                     WIYPVIDENIDIEIDEKDVRIDTYRASGAGGQHVNTTDSAVRITHEPTGIVVQCQNQR
                     SQHKNRATAWAMLRARLYEAELKRREEEALAAAASKSEIGWGHQIRSYVLQPYQLVKD
                     LRTGVESTNPTAVLDGDIDAFIEAALAERIHADQKPENKPA"
     gene            complement(4208..4765)
                     /locus_tag="AC16Y7_00355"
     CDS             complement(4208..4765)
                     /locus_tag="AC16Y7_00355"
                     /inference="COORDINATES: protein motif:HMM:NF016408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR5707861.1"
                     /translation="MFTKKPESAEAVTKLGERPSSSEKSSPFTERPATYSKNPSPSPS
                     AGANSGASKMVPSIIGEDLTVIGNVASKGEIQVDGEIQGDIDCGSLLLGDKAQINGSV
                     RAEDVVVRGKVTGSVRGLRVTLQSQSHVEGDIHHQSLAIEQGAYFEGKSRRSDDPMAD
                     GKKPSARSAKSADSEENPFSGRAAE"
     gene            5222..>6039
                     /locus_tag="AC16Y7_00360"
     CDS             5222..>6039
                     /locus_tag="AC16Y7_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707862.1"
                     /translation="MGEPIAGALAKLTAGSGKALRALPGQGRELVKRVPPPVQIQLAK
                     LSTRSAHLFREIFAGILVVGLIAIVFGYGRLAKGPVSLPNFVEPIETAINDQLTDLHV
                     KIDDAVLQRSDDGPGVLFRLRDIRLIDKDGEVVAQAPLAAIGMSGSALLSGRVAPGSV
                     DFIGPRLLMSYTDDKGLSLAFSKNDAQSDQLIRGALPAGKPPSDALVPPAAPSQAPPA
                     SSPRQFDLTKTVEDVFDRTRAGDTSYLTRFGFKDAIVVLNNDGAQTLWQVPDFA"
BASE COUNT         1145 a         1881 c         1914 g         1099 t
ORIGIN      
        1 gagaagcaac tcacctccga ggcttggcgc gaccggatgg ccacatcggt gacggcggcg
       61 atcgacggct atttcaacaa gcgcatcgcc cgcagcccgt tctgagcggg aaggtctggc
      121 cctaaggctg ctcccgcaag gtcgggaaat tccatgattt cggacgactc ttgcgtttta
      181 ttgccatatt cttggctagg ttggtttggg tgccccgcca ggcggaacgc ggggtcggga
      241 cggtaggaaa ttatggacat cacgacacca gtcacgccgc ccaagcgagg gcggaaaaag
      301 aaacgtcgcg gcttcttcct gcgctttctc ggcttcatgt tcgcagcggg catgatcctg
      361 gccattggcg tggcgggcgc ggttgcgttc gtgttgtgga aggcatcgca agacctgccg
      421 gactacgagg tgctcgccaa atacgagccg cctgttatga cgcgcatcca cgccaatgac
      481 ggccggctca tttccgagtt tgcacgccag cgacgtatct acgtgccgat taccgcagtt
      541 ccgaagcgcg tgatcgaggc attcctctcc gccgaggaca agaatttcta tcagcatggc
      601 ggcctcgaca ttcagggcat catccgcgcc atcgtcacca acatgagcgc gatgcaagcc
      661 ggcggcggcc atatggtcgg cgcttcgacc atcacccagc aggtggcgaa gaacttcctt
      721 ctgtcgagtg agcagaacat cgagcgcaag ctcaaggagg cgattctcgc catccgcatc
      781 gagcgcgcct tcactaagga tcagattctc gagctttacc taaacgagat ctatctcggc
      841 ggcggtgctt acggcgtggc ggcagctgcg cagagatatt ggggcaaagc gctgaacgag
      901 ctgtcttggg cggaagcggc ctatctcgca ctcctgccga aggcgccaag ccggtatgac
      961 ccctggaagc acgctgaacg cgcgctgacg cggcgcaact gggtgatcga ccggatcgtc
     1021 gagaacggct ttgcgacgcc tgaggaaggc gagcaggcca aggccacgcc gctcggcgtg
     1081 ctggcgaaga agtacggtcc gcagattcac gcctcggagt atttcgcaga ggaagtgcgc
     1141 cgcgagatcc tcgacaagtt cggcgaggac aagctttacg gtggcggcct gtcggtccgc
     1201 accacgctcg acccgcggct ccaagcgatc gcccgcaagg cgctggtcaa ggggctaacc
     1261 aaatacgacc gcgcccatgc aggctggcgc ggcgtcgaca agaccatcga aatctccggc
     1321 gattggggca aggcgcttgc cgaaattccg gtgtggaagg atatcgcgcc ctggaaacgg
     1381 gcggtcgttc tcgaagtgtc gggcgatcag gcaacgatcg gcatccggcc cgggcgcgac
     1441 aaggaaggcc gcttggtcga agagcgggag accggcgtga tcccggcgtc gcaagtcaag
     1501 tggacgcgta agtccatcaa gtccgcgctg aagccgggcg atgtgatcta cgtcgcgccg
     1561 ctcgagccca agaagaacaa ggatggcgag gttgcgccgg cggaaacggt tgcgggcaag
     1621 tggtcgctcc agcagccccc caaggtcagc ggcgccatta cggctataga cccgcacacg
     1681 ggtcgcgtgc tcgccatcgt aggcggcttc agcttccatc agagccagtt caaccgcgcc
     1741 acccaagcgc tgcgtcagcc cggctcctcg ttcaagccgc tcatctatct cacggccatc
     1801 gacaacggct atacgccgtc gagcatcatc gtcgacggac gcatttgtgt gagccaaggc
     1861 cgcggcatgc cgccctggtg ccccaagaac tactcgggtg gaggcgcagg cccctcgacc
     1921 ttgcgccgcg gcatcgagaa gtcgcgcaac ctgatgacgg tgcgtttgtc gcgggatatc
     1981 ggcatgcccg tcattgccga atattcgaac cgcttcgggg tttacgacaa tctggtaccg
     2041 gcgctcgcta tgtcgctcgg gtcgggtgag accacgctgc tgcgcatggt taacgcctac
     2101 tcgatgatcg ccaatggcgg caagaaggtc gaagcgacct tcatcgaccg cgtccaggat
     2161 cgctatggcc gcaccgtctg gcgtcacgac aagcgggact gcagtttgtg cagcgcgcgc
     2221 gagtggaccg gacagggcga gcccgagctt atcgatgtcc gcgatcaggt cgtcgacccg
     2281 gtttccgcct atcagatggt tggcatcatg gaaggtgtcg ttcagcgtgg aacggcaacg
     2341 aggctgagag cgttgaagcg gccgctggcc ggcaagaccg gcacgaccaa cgattacaag
     2401 gacgcctggt tcgtcggcta tacgcccgac atggccgtgg gcgtctatgt cggctacgac
     2461 cagccgaagc ccatgggccg cagcgccacc ggcggcggtc tggccgcgcc catcgtcaag
     2521 gacttcctcg ccgaggctct caaggacgtt ccgccggccg aattccggcc gcctgaaggg
     2581 gccgtcttgg tccgcgtcaa ctccaagacg ggtcttccgg cccgtggtgg tggaggcggc
     2641 acgatcgtcg aggctttcaa gccgaacgag gttccccccg gtgcgattcc gctgggtcga
     2701 gatggcaagc cccagggcca agcggccgag gaaggtgcgc agccggacgg gttcccccag
     2761 tatccagctg aagctcaggc gggtgcgcca gggcaaccgg ctggtgccca ggccgcagcg
     2821 ccgcaggccg cgcctcagcc gccgcctcag gcctccgcgc cgccaccgcg ccaacgccgc
     2881 gcaccgcggc gcgagcgcgg attgttccgc ggtttctttt agagcttcgt tcaggggcga
     2941 ggaccgggcg cgccgtccgc ccggctccgt tctattgaat ttcgacatca aactttgagg
     3001 actgcgcatg cgcgccgaaa ccgaggcggt cgtcaacgac atcaaagagt ctctgtcttt
     3061 gctgcggagg catctttgac cccgatcagg ccaaagcacg gctgagtgaa ctcgaccgcg
     3121 aggccgaagc gcccgacttc tggaacgatg caggccgcgc gcaaactctg atgcgcgaac
     3181 gccagcagat cgagacctcc ctcaacgcct tcgatcgcct cgcaaacgaa ctcgacgaca
     3241 atctcacgct cgtctccatg ggcgaggagg agggggacga cagcatcgtc gccgaagcgg
     3301 aggccgcgtt gcaaaagctg cgggccgaag cccacacccg cgaggtggag gcgctgctgt
     3361 cgggggaggc ggacgccaac gacgccttta tcgagattca cgccggcgcc ggtggcaccg
     3421 aaagccaaga ctgggcgcaa atgcttgagc gcatgtatct gcgctggtcg gaagcgcggg
     3481 gctacaagac cgagctgatc ggcgagcatt acggcgatga agccggcatc aagtcagcca
     3541 cgatcctggt caagggcttg aacgcctatg gttggctgaa gaccgagtca ggcgtgcacc
     3601 ggctggtccg gatttcgccg ttcgactcca atgcgcgccg gcacacgagc tttgcttcgg
     3661 tgtggatcta ccctgtcatc gacgagaaca tcgacatcga gattgatgag aaggatgtcc
     3721 gcatcgacac ctatcgggcg tcgggcgccg gaggtcagca cgtcaacaca actgacagcg
     3781 ccgtgcgcat cacccatgag ccgaccggca ttgtcgtgca gtgtcaaaac cagcggtcgc
     3841 agcacaagaa ccgcgctacg gcatgggcca tgttgcgcgc gcggctgtat gaagccgagc
     3901 tcaagcggcg ggaggaggag gcgctggccg ccgccgcgtc gaagtcagag atcggatggg
     3961 gccaccaaat ccggtcctac gtgctccagc cctatcagct ggtgaaggat ctgcgcacgg
     4021 gcgtggagag tacgaatccg acggctgtgc tcgacggcga tatcgacgcc tttatcgagg
     4081 cggcgctcgc ggagcgcatt cacgccgacc agaagccgga aaataagccg gcctaaaaac
     4141 cagcctaaaa agcaatgagc ggagggccgc aaccggcacc ccgctcatcg cgcaatctga
     4201 tagtccgcta ctccgcggcg cggcccgaga acggattttc ttccgagtcg gctgactttg
     4261 ccgaacgcgc tgacggtttc tttccgtcgg ccatcggatc gtcggaccgg cgtgacttcc
     4321 cttcgaagta ggccccttgc tcgatagcaa ggctctgatg gtggatgtcg ccttcgacat
     4381 gggactgcga ttggagcgtc acccgaaggc cacggaccga tcccgtcacc ttgccgcgca
     4441 ccacgacgtc ttcggcccgg acggatccat tgatttgggc cttgtcgcca agcagcaggg
     4501 agccacagtc gatatcgcct tgaatctcgc catcgacctg gatctcgccc ttcgacgcga
     4561 cgttaccgat caccgtcagg tcctcgccga tgatcgaggg gaccatcttg ctcgcgccgg
     4621 aattggctcc agcgctcgga cttgggcttg ggttctttga gtaggtcgcg ggccgttcgg
     4681 tgaacggcga tgatttctcc gagctgctcg gtcgctcacc aagcttggtg acagcctcgg
     4741 cagactcagg cttcttcgtg aacatacgtg ggtacctccc cattcaatac gagtgacgca
     4801 gcgcaagacg caaacgagta ccagatcccg cgacaggtga cagctaaact gctgaaatgg
     4861 tgtggaccca gtgttcaaac tcctgaccgt gacaatatcc cctcgaccta tccttgtcat
     4921 gggattctct gtcccagaaa gggcccttgc cgagacccgg ccaggcccgg tattgggctt
     4981 gaatcgcctt ggtttggccg attttcgtcc ctcttatgcc ttgagtcctt ggcctcttgg
     5041 ggtacagagg cagaaggcgg gattaaaggt gcgccttggg ggtgccggtc aagttgccat
     5101 gagcagacca gggctcgctg gtgtaaaacc agtagcgagg tcggttgttg cgcatggctg
     5161 cgtgttatcc gcggccgcgc agccaagcgg gcttggccag atttatgggg cctgtgactg
     5221 aatgggcgaa ccgatcgcag gggctctggc taagctgacc gccggcagcg gtaaagccct
     5281 tcgcgctctg cccggccagg gccgggagct ggtgaagcgc gtccctccgc ctgttcagat
     5341 ccagcttgcc aagctgtcga cccgcagtgc ccatctcttc cgtgagattt tcgccggcat
     5401 cttggtcgtc ggcttgattg ccattgtttt tggctatggc cgcttagcca aggggccggt
     5461 ttcgcttccg aacttcgttg agcccatcga gacggcgatc aacgatcagt tgacggatct
     5521 gcacgtcaag atcgacgacg cggtgctcca gcgcagtgac gatgggcccg gcgtgttgtt
     5581 ccgccttcgc gatattcgcc tcatcgacaa ggacggcgag gtcgtcgcgc aagccccgct
     5641 cgccgctatc ggcatgagtg gcagtgcgct cctttcggga cgagtcgccc ccggaagcgt
     5701 cgacttcatc ggcccgcggc ttctgatgtc ctatacggac gacaaaggcc tctccttggc
     5761 cttctccaag aacgacgcgc aaagcgatca gttgatccgc ggcgcacttc ccgcaggtaa
     5821 accgccgtct gatgcgttgg tgccgccggc cgcgccctct caagcgccgc cggccagcag
     5881 ccctcgtcag ttcgatctga ccaaaaccgt ggaggatgtg ttcgaccgca cgagggctgg
     5941 ggacacgtcc tatctcaccc gtttcggttt caaggacgcg atcgttgttc tcaacaacga
     6001 tggcgcgcag acgctctggc aggttcccga ctttgccat
//
LOCUS       DBNHVU010000010         5679 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_452238, whole genome shotgun sequence.
ACCESSION   DBNHVU010000010 DBNHVU010000000
VERSION     DBNHVU010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5679)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5679)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5679
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_452238"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..307)
                     /locus_tag="AC16Y7_00365"
     CDS             complement(5..307)
                     /locus_tag="AC16Y7_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR5707863.1"
                     /translation="MDCRVLKPIAVAALSAVILSAAAPPARAACVPWKSAGALISQNS
                     LVPGNVIYQKVQSKTGGKVISATLCQNGSNFVYKVVVLGKKGDVKNLTINARTGQF"
     gene            complement(545..1027)
                     /locus_tag="AC16Y7_00370"
     CDS             complement(545..1027)
                     /locus_tag="AC16Y7_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HIR5707864.1"
                     /translation="MSLWTKLKDLVEGIDTDAGRVKDLRGEELRLAAGALLIGAGGID
                     GNFDPDEKRKVNDLLQRRFDLEPKEVKALFDDAAAQERDAVDLYRFTSVLCRELDQDG
                     RKRIVEMLWEVVMADGVVDEFESNLVWRVAELIGVSTRDRVTIRKMVEARLGMSSQAT
                     "
     gene            complement(1130..1549)
                     /locus_tag="AC16Y7_00375"
     CDS             complement(1130..1549)
                     /locus_tag="AC16Y7_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HIR5707865.1"
                     /translation="MTAACWALQRSMYQTLSNASELTSLLGGDGIYSNPPPAAEFPFI
                     TIGQTVDLDWSTGTEDGNEHSITLHIWSRADSAMEVHEITEVVRNLLHDQALTLDDHE
                     LVNLRHEFSQARIDPDGETLHGIVRYRAVTEPMQQAA"
     gene            2519..4465
                     /locus_tag="AC16Y7_00380"
     CDS             2519..4465
                     /locus_tag="AC16Y7_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020397560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR5707866.1"
                     /translation="MNITPKILTLLAVTSCVVPVSALAEEEKVGGQVGEMSLSAGDVG
                     LFKRENFSPYAGRQFPNRPLWGDTHLHTSNSLDARAFGVTLDPEQAYRFARGEEIKTS
                     HGLNVRLSRPLDWLVVSDHSDGMGAMNEIVAGDVTLMRDPQLRDWNKRINEGGDEALQ
                     ATMEVIESFAGISEIKMPAAALDPRFVQTVWDEYLETADKYNEPGRFTTIIGYEWTPT
                     EGGNNLHRNVLYRDGAIRAQQMLPFTTESSFNPEDLWKWMEAYEDKTGGRVLALAHNG
                     NVSNGLMFPVETNPATGKPLTGDYAETRAKWEPMYEVTQIKGDGEAHPFLSPNDEFAD
                     YETWDKSNLGPVPKEDDMLQYEYAREALKNGLKLEDGLGTNPYKFGMVGSTDSHTALA
                     TAQEDNFFGKHAGVEPEPHRWEHVVGEFGPTRILGWEMAASGLAAVWAPENTRRAIFD
                     AMQRKEVYGTTGSRIGLRFFGGWDFNDSDATSRTPAEIGYAKGVPMGGDLENPPEGKS
                     PSFLVAALKDPIGANLDRIQIVKGWLDADGKTQEKVYDVVWSGARKPGSDGKLPAVGD
                     TVDVEKATWTNTIGAPELITVWEDPDFDPNQRAFYYARVIEIPTPRWTAYDAKRFDVE
                     MSDDVPMTTQERAYTSPIWYTPKG"
     gene            4870..>5679
                     /locus_tag="AC16Y7_00385"
     CDS             4870..>5679
                     /locus_tag="AC16Y7_00385"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat domain-containing protein"
                     /protein_id="HIR5707867.1"
                     /translation="MGRNILVVLTAVLVLVVAGAYVVLVPAEAVRPPPATVAKPNLVS
                     IPTPFTHRWSKGTSHPLLAAAAIDVDDSGRDAVFLGGSDGQPDALLAWRDGKLVDIAS
                     EVGLGDNQATYGALSIDINHDGRIDLLTVGHSGLVLWINYGRRFERKRLDVEFPADAV
                     PMALTAGDFDRDGQVDLYISLFVAPEKFRSPVFNDPTHAKRNVLLRNEGDLKFANVTD
                     DVSGGLQNTFTASFIDLDNDHWLDLVLAQNTGQIEFLRNLGNGKFARLDFPF"
BASE COUNT         1129 a         1749 c         1712 g         1089 t
ORIGIN      
        1 cctcctagaa ttgccccgtg cgggcgttga tggtgaggtt cttcacgtcg cccttcttgc
       61 ccagcacgac aaccttgtaa acaaagttcg agccgttttg gcacagggtg gcggagatca
      121 ccttgccgcc ggttttcgac tggacctttt gatagatgac gttgccagga accagcgagt
      181 tctgcgagat gagtgcgccg gccgatttcc acggaacgca cgcagcccgc gccggcggcg
      241 ccgccgcgga cagaatgacc gcgctcaacg ccgcgacggc aattggcttc aaaacccgac
      301 agtccatctc agtcccaaca tccattcttg ggaggtaacc ctaagggacc gccaatgaac
      361 ggcgcatgaa cggttggtca agcacctttc gagcgaccgg atttcctcgc ggcaggcttg
      421 gcggcatcgt ccacctaggc gccgagcgcc gcaagcccca aaccgcaagc tgcaaacctc
      481 gtgtttaaac aacaaaaaag ggaggccaaa gccgccctcg aaaatctcat cgctaaaaaa
      541 ccggtcaggt cgcctgggac gacatcccca gccgcgcctc gaccatcttg cgtattgtca
      601 cgcggtcgcg cgtcgacacg ccgatcagct cggcgacgcg ccagacgagg ttcgattcaa
      661 actcgtccac gacgccgtcc gccatcacca cttcccacaa catctcgaca atgcgcttgc
      721 ggccgtcttg atcgagctcg cggcagagca cgctggtgaa ccggtagagg tccaccgcat
      781 cgcgctcctg cgccgccgca tcgtcaaaca gcgccttcac ttccttgggc tcaagatcga
      841 agcgccgctg gagcaggtcg ttgaccttcc gcttctcgtc cgggtcgaag ttcccgtcga
      901 tgccgccagc gccaatcaga agcgcgcccg ccgctagccg cagttcctct ccgcgcagat
      961 ccttcacgcg cccagcgtcc gtatcgatcc cctcgacgag atccttcagc ttcgtccaca
     1021 acgacatcca gtcctcccac gcaagcaact ctgccctgag gtcctacaaa acgacaatgg
     1081 ccgggacaag cccggccact gccatttgga tactgggggt gtagtgcgtc taagctgctt
     1141 gctgcatcgg ctcggtcacg gcgcgatagc gcacgatgcc gtgcagggtt tcaccgtccg
     1201 gatcgattcg cgcttgcgag aactcgtgcc ggaggttgac cagttcgtgg tcgtcgagcg
     1261 tcagggcctg gtcgtgcagc agattgcgca cgacctctgt gatttcgtgc acttccatgg
     1321 cgctgtcagc gcgcgaccag atgtgcagcg tgatgctgtg ttcgttgcca tcctcggtgc
     1381 ccgtgctcca atccaggtcc acggtctgcc cgatggtgat aaaggggaat tcggccgccg
     1441 gcggcggatt gctatagatg ccgtcgccgc caagaagcga cgtcagctca gacgcgttgg
     1501 agagcgtctg gtacatgctg cgttggagcg cccaacacgc ggccgtcata tgggatcctc
     1561 catcagttaa cgaacatgta gggaccggaa cgccgaatac aatggcccgc tcttaccgcc
     1621 tcgccagtgc ggcacgtaaa gtatttgtga gactttgctt aacgcgcggt aaacgcgcgc
     1681 gaaaaatcgg ccaaagccag gggggtcgcg ggctacgttg gagtggcgat acgctgcaga
     1741 agacgataca cgaacggtag acggcacgcc tttggaacct accgttgctg cttcgcagca
     1801 attgggttca ttttgcaaaa cggcagccaa ggcgacctag cggttctaag ggtctcgcct
     1861 tcgtcgtttg tccctaagtc gaactcccgg gcccatctcg tcgtccgcag ggatgaattc
     1921 cacacccgct tcctccagag ccgtctggag cttccagtag gtgtccgccg ctccgcggac
     1981 gtcaggggag gcttcaattc gctttacggt tgctagcccc gcagaagcga gagcggcaag
     2041 atctcgttga cttatgccaa ccagtgcacg ggctgctcga atctgtgaag cgccattggg
     2101 ccaaggtagg catcgcgtcg cagacggtga gcgtcggatt gctcggacgt tggtagaggg
     2161 cttagcaaag tagcggactt accacatggg cctgacttcc gttttttggc actcagcgga
     2221 cgtgcccgtg gaccggccgc taggttggct cttgacccaa agcgggcatc gagagcttgt
     2281 tggctatcgg ccggttggtg cctctgccgc ccgatcactc gagactggcg ggcggcgggg
     2341 agcatcctga cagcgggaaa aagattgtga gcaggccgag atcgccttcc agagagttct
     2401 cgacatcaaa gtcgtgcatc caggttagtt gcgtgctccc ccggctccgc gttaagtcta
     2461 cacgtcgccc atctggtaca cgcctaaggg ctagccacag tcagggagga ctggtgcact
     2521 gaacatcacc ccgaagattc tgacactgct tgcggtaaca tcctgcgtcg tccccgtttc
     2581 ggcgctcgca gaggaggaga aggttggcgg ccaggtcggc gagatgtcgc tctcggccgg
     2641 cgatgttggc ttgttcaagc gggagaactt ctcgccctac gcagggcgcc agtttccgaa
     2701 ccgcccgctc tggggggaca cgcacctgca tacctcgaac tcgctggacg cacgggcctt
     2761 cggtgtgacg ctcgacccgg agcaagccta ccgctttgcc cgcggcgagg aaatcaagac
     2821 cagccacggc ctcaatgtgc ggctgtcgcg gccgcttgat tggctcgtgg tctccgatca
     2881 ctccgacggc atgggcgcca tgaatgagat cgtcgcaggt gacgtcacgc tcatgcggga
     2941 ccctcagctg cgcgactgga acaagcgtat caacgaaggc ggagacgagg cgctgcaagc
     3001 cactatggaa gtgatcgagt ccttcgccgg gatcagcgag atcaagatgc cggccgcggc
     3061 gctcgatcca cggttcgttc agacagtttg ggacgagtac ctcgagacgg ccgacaagta
     3121 caacgagcct ggcaggttca cgacgatcat tggctatgag tggaccccga cggagggcgg
     3181 caacaatctg caccgcaacg ttctgtaccg cgacggagcc atccgcgcgc agcagatgtt
     3241 gccgttcacc acggagagca gtttcaatcc tgaagacctc tggaagtgga tggaggctta
     3301 cgaggacaag actggcggcc gcgtgctggc gctggcgcat aacggcaacg tctcaaacgg
     3361 cctcatgttt cctgtcgaga ccaacccggc aaccggcaag ccgttgaccg gcgactatgc
     3421 cgagacccgg gcgaaatggg agccgatgta cgaggtcacc cagatcaagg gcgacggcga
     3481 ggcacatccc ttcctgtcgc cgaatgacga gttcgcggac tacgagacct gggacaagtc
     3541 gaaccttggc ccagtcccga aagaagacga catgctgcaa tacgagtatg cgcgcgaggc
     3601 tctgaagaac gggctgaagc ttgaggacgg tcttggcacg aacccgtaca agttcggcat
     3661 ggttggctcc accgacagcc acaccgcctt ggccacggcg caggaggaca acttcttcgg
     3721 caagcacgcc ggcgtcgaac cggagccgca ccgctgggag cacgtggtcg gcgagttcgg
     3781 cccgacccga atcttgggat gggagatggc ggcctcgggt cttgcggccg tttgggcgcc
     3841 tgagaatacg cggcgcgcca tcttcgacgc gatgcagcgt aaggaggtct atggcaccac
     3901 cggatcgcgt atcggcttgc ggttcttcgg gggctgggac ttcaacgaca gcgacgccac
     3961 cagccgaacg ccggccgaga tcggttatgc caaaggcgta cccatgggtg gcgacctgga
     4021 gaatccgcct gagggcaagt ctccaagctt ccttgtcgcc gcgctgaagg acccgatcgg
     4081 cgccaatctc gatcgcatcc agatcgtcaa gggttggctc gacgccgacg gcaagacgca
     4141 agagaaggtc tatgacgtcg tctggtcagg tgcgcgcaaa ccgggttccg acggcaagct
     4201 gccggcagtc ggggacaccg tggatgtcga gaaggccact tggaccaaca cgattggcgc
     4261 ccctgagctg atcactgtct gggaagaccc ggacttcgat ccgaaccagc gtgcgttcta
     4321 ctatgcgcgg gtgattgaga tccctacgcc ccgctggacc gcctatgacg ccaagcgctt
     4381 cgatgttgag atgtcggacg acgttcccat gaccacccaa gaacgcgcct acacgtcgcc
     4441 catttggtac acgcctaagg gctaagtatc gaatgctaga gaaggtttta gggcagctcg
     4501 ctgagggccg cctttttgct gcgaaagact ccgctgcgat tcacgtgaat gtccacttct
     4561 ggcacttagc ggacctctat gccgaaaccg agcaagtccc ctcttgggcc caaaccagac
     4621 gtggtctggc caaggcgcaa gggccgcttc tgaccctaag cggacatgtt cagaccagta
     4681 cagcaggcga tggctttgcc tggacaaggg acaacgcgcc agtcagagat aatggcttgg
     4741 tctggattgt ctctccgatg gctatgagag aagctgattg ccggtctcgg gttctgggct
     4801 ctgcgcagaa gcacgtattg cgctcagcgg ggaccgatga cgaggcccac aatcaggggg
     4861 atctcaatca tggggcgcaa catcttggtt gttctaaccg cagtgcttgt tctcgtagtc
     4921 gcgggggcct acgtcgtgct agtgcccgcc gaggctgttc gcccgccccc tgcgaccgtc
     4981 gccaaaccca atctcgtgtc gatccccacc cccttcacac accggtggtc caaggggacc
     5041 agtcatccgc tgcttgccgc tgcggccatc gatgtcgacg actccgggcg cgatgcggtt
     5101 tttctcggcg gctccgacgg gcaaccggac gcgctgcttg cgtggcggga tggcaagctt
     5161 gtcgacatcg ctagtgaggt cggtctcggt gacaatcagg cgacttacgg cgcgctatcc
     5221 atagacatca accatgacgg gcgcatcgac cttttgaccg tcgggcactc gggacttgtt
     5281 ctctggatca attatggtcg gcgtttcgag cgcaagcggc ttgacgtcga gtttccggcc
     5341 gatgcagtgc cgatggctct cacggcgggc gatttcgacc gcgatggtca ggtcgacctc
     5401 tacatcagcc tgttcgttgc gccggaaaaa ttccgctcgc ccgtgttcaa cgatccgacc
     5461 cacgccaagc gcaacgtact gcttcgtaat gaaggtgatc ttaaattcgc caacgtgacg
     5521 gacgacgtat cgggcgggct gcagaacacg ttcactgcat cgttcatcga ccttgataac
     5581 gaccactggc ttgacctcgt gttggctcag aacaccggcc aaatcgaatt tctacgcaac
     5641 ctcggcaacg gtaaattcgc tcggctagat tttcccttc
//
LOCUS       DBNHVU010000011         8447 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_304321, whole genome shotgun sequence.
ACCESSION   DBNHVU010000011 DBNHVU010000000
VERSION     DBNHVU010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8447)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8447)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8447
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_304321"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="AC16Y7_00390"
     CDS             <1..277
                     /locus_tag="AC16Y7_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine amidotransferase-related protein"
                     /protein_id="HIR5707868.1"
                     /translation="KVAEIYGGTEISERHRHRYEVNMRYRNKLEAAGLRFSGLSPDGL
                     LPEIVEIPEHPWFIGVQFHPELKSRPFAPHPLFESFIGAAVEQSRLV"
     gene            293..715
                     /locus_tag="AC16Y7_00395"
     CDS             293..715
                     /locus_tag="AC16Y7_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR5707869.1"
                     /translation="MPETRHKGSCACGQVRVEARGEPYRIGLCHCLTCRKAHGAPFSF
                     YAVFDPEAVSVDGDVIAFASTEHVRRYACAACGAPVYSTYGRTDEFYLHPGSLDDIGG
                     FEPTYELWTIRREPWLPEIPSVKYRYEKSRPKWRRHEN"
     gene            774..1625
                     /gene="kdsA"
                     /locus_tag="AC16Y7_00400"
     CDS             774..1625
                     /gene="kdsA"
                     /locus_tag="AC16Y7_00400"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HIR5707870.1"
                     /translation="MNEAMKPAPAAEVAIGNVRFGNDLPLALMAGPCVLESRAHALEM
                     ASALKEIAAKLGVGLVYKSSFDKANRTSAAGARGLGLKQALPIFAEVREVTGLPVVTD
                     VHEPEQCAPAAEAADVLQIPAFLCRQTDLLVAAAKTGKPVNVKKGQFLAPWDMRHVVA
                     KVTSAGNPNVLVTERGVSFGYNTLVSDMRALPILAETGAPVIFDATHSVQQPGGQGAS
                     SGGERRFVPVLSRAATAVGVSGLFIETHQDPDAAPSDGPNMVPLKELEPLLAELIEID
                     RLVKSQA"
     gene            complement(1632..2612)
                     /locus_tag="AC16Y7_00405"
     CDS             complement(1632..2612)
                     /locus_tag="AC16Y7_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HIR5707871.1"
                     /translation="MTDLVITNGDVAGELLRKTLIGAEVLPWRDVLHEGPVPLTDTRE
                     ALAKARAEYLAGLAGSDVGTIEFEMTARDRGLGISAEFDRVMLWFEHDLYDQLQLLQV
                     LDWFADHPPQAGLLSLVQVEDYIGRQEPDAIPQLAASAVPVTQAQLDLAKRAWAAFRQ
                     PTPEAWAALLDEDLSVLPFLHAAVVRMLEELPGVDGLSRTERQMLAAIEADESATALG
                     AFVAVQKMEDAEFMGDWSFWRLLDQLALADEPLIAGLESAPFQYQDPELAKAYLTSRL
                     SLTSLGKAVLAGGADWAKHRAVDHWWGGTHLTEDNLWRWDGEAARLIAPA"
     gene            complement(2617..3078)
                     /gene="queF"
                     /locus_tag="AC16Y7_00410"
     CDS             complement(2617..3078)
                     /gene="queF"
                     /locus_tag="AC16Y7_00410"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HIR5707872.1"
                     /translation="MTRKITGLRQLGQETKVPGSPEEAVLERVPNPHADTAYVARFTA
                     PEFTCLCPVTGQPDFAHFVIDYAPGAWLLESKSFKLFLASFRNASAFHEDATLTIGKQ
                     IVDLIEPSWLRIGGYWYPRGGIPIDVFWQHGTPPEGVWIPDQGVAPYRGRG"
     gene            complement(3082..3462)
                     /locus_tag="AC16Y7_00415"
     CDS             complement(3082..3462)
                     /locus_tag="AC16Y7_00415"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl trahydropterin synthase family
                     protein"
                     /protein_id="HIR5707873.1"
                     /translation="MRIYKEFFFEAAHFLPSAPPGHPNSRVHGHSFRARITVDGEPDE
                     DTGIIVHFDELDAAVAEARDALDHRMLNEVEGLQYPTLERIAMWLWDRLHNRLPGLVE
                     IVVARDSCHEGCVYHGPPMRLAAE"
     gene            complement(3522..4157)
                     /gene="queE"
                     /locus_tag="AC16Y7_00420"
     CDS             complement(3522..4157)
                     /gene="queE"
                     /locus_tag="AC16Y7_00420"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HIR5707874.1"
                     /translation="MYTVKEIYYTLQGEGAQAGRPAVFCRFAGCNLWSGREVDRAQAI
                     CDFCDTDFVGTDGPGGGKFAGPDALAVACRETWRGNSGTPPFVVLTGGEPMLQVDKAL
                     VDAFHTVGFEVAIETNGTLPVLTSIDWVCVSPKPGAPLRQRSGDELKLVYPQDRLWPE
                     EVQTLNFSHYLLQPLDDGTGGVKHLQAAIDYCLAHPKWRLSLQTHKLLGLP"
     gene            complement(4454..5200)
                     /gene="queC"
                     /locus_tag="AC16Y7_00425"
     CDS             complement(4454..5200)
                     /gene="queC"
                     /locus_tag="AC16Y7_00425"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HIR5707875.1"
                     /translation="MPEKTSDAKKSALVLHSGGQDSTVCLAQALDRYERVETVGFNYG
                     QRHGVELECRVRVRAAIERHFPDWAKRLGPDHMIDISSFGAIGDTALTSDAEIVMLDS
                     GLPSSFVPGRNLAFFVYAAALGYRRGLNVLVGGMCETDYSGYPDCRDATLRALQESIA
                     LGTDAPFVIETPLMWLSKAQTWDLAQEVGGEALVHILIEETHTCYKGERGTRYDWGYG
                     CGECPACELRAKGYWEWQASSSGRATKAAK"
     gene            5301..6578
                     /gene="eno"
                     /locus_tag="AC16Y7_00430"
     CDS             5301..6578
                     /gene="eno"
                     /locus_tag="AC16Y7_00430"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR5707876.1"
                     /translation="MTAIANIVGREILDSRGNPTVEVDVVLEGGHHGHAAVPSGASTG
                     AHEAHELRDGGTRYGGKGVQKAVEAVNGEIRKALDGFDADDQRGLDKLLCDLDGTENK
                     KRLGANAILGVSLAAAKAAASSHEQPLYRYLGGEEATLLPVPMMNIVNGGMHADNPID
                     FQEFMIVPVGAPSIAEAVRMGAEIFHTLKADLKAAGYGTAVGDEGGFAPNLASTRQGL
                     DFVMESIGKAGYHPGKDVYLALDVAATEFYKDGSYVLAGENRSLTSDEMAAYLADLVA
                     NYPIFSIEDGMSEDDWDGWRSLTEMLGSRVQLVGDDLFVTNVTRLQEGIDRNIANSIL
                     IKVNQIGTLSETLDAVKMAHDAGYTAVMSHRSGETEDTTIADLAVATNCGQIKTGSLA
                     RSDRVAKYNQLIRIEEELGPRARYAGAAVLRTA"
     gene            6651..6974
                     /locus_tag="AC16Y7_00435"
     CDS             6651..6974
                     /locus_tag="AC16Y7_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIR5707877.1"
                     /translation="MRFRAFIFPLLALSAGGYFGYHLQNGDHGLTSHADLERRKDVLE
                     GELAGLREVRLRIERDVALLEPDSLDPDMLDERARAILNLAHRDDLVMMKRRAPDPTG
                     PLRKP"
     gene            7106..8119
                     /gene="pdhA"
                     /locus_tag="AC16Y7_00440"
     CDS             7106..8119
                     /gene="pdhA"
                     /locus_tag="AC16Y7_00440"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HIR5707878.1"
                     /translation="MDQKPFGTPASAPPKGAPLAVEDELNAYRDMLLIRRFEEKAGQL
                     YGMGFVAGFCHLYIGQEAVVTGMTMAIKPGDQIITAYRDHGHMLATGMDPKGVMAELT
                     GRAHGYSKGKGGSMHMFSVERNFFGGHGIVGTPAPLGTGIAFANKYRDNGNVCMAFFG
                     EGAANQGQVYESFNMASLWKLPIVYVIENNHYALGTSVERASAEWKELHHRGLAFGIP
                     GEEIDGMDVAMVKAAGEWALAHAREGKGPVIIEALTYRYRGHSMSDPAKYRTREEVQD
                     MRQHHDPIELVRKRLLDAGKSEDELKAIDRDIRAVVNEAAEFAQSDPEPDPSELYTDV
                     VAT"
     gene            8133..>8447
                     /locus_tag="AC16Y7_00445"
     CDS             8133..>8447
                     /locus_tag="AC16Y7_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR5707879.1"
                     /translation="MSTEILMPALSPTMEEGTLAKWHVREGDVVHAGDVIAEIETDKA
                     TMEVEAIDDGTLDKILVPEGAEHIPVNQPIAILMTEGEDDVSAIEVMSQAAPADAFQA
                     PPP"
BASE COUNT         1628 a         2709 c         2630 g         1480 t
ORIGIN      
        1 caaggtggcg gagatctatg gcggcacgga aatctccgaa cgccatcggc accgttacga
       61 agtcaacatg cgctaccgca ataagctgga agcggcgggc ttgcgattct ccggcctttc
      121 tcccgacggc cttctgccgg agatcgtcga gattcccgag catccgtggt tcatcggcgt
      181 tcagttccat cccgaactga agtcgcggcc cttcgcgccg cacccgctgt tcgagtcgtt
      241 cattggcgcg gcggtcgagc agagccgctt ggtctagtgc cgtcgacctg acatgcccga
      301 gacacgtcac aagggttcct gcgcctgcgg tcaggtgcgc gtcgaggcga ggggggagcc
      361 gtaccgcatt ggcctgtgcc actgcctgac ctgccggaaa gcgcatggcg cgccgttcag
      421 cttctatgcg gtgttcgacc cggaggcggt gagcgtcgac ggcgacgtga ttgccttcgc
      481 cagcaccgaa catgtccgcc gctatgcgtg cgccgcgtgc ggggcgccgg tctattcgac
      541 ttacgggcgg acagacgagt tctatctgca tcccggctcg ctcgacgaca tcgggggctt
      601 cgagccgacc tacgagcttt ggaccatacg gcgcgagcct tggctgcctg aaatcccgag
      661 cgtgaagtat cgctatgaga agagccgccc caaatggcgg cgtcacgaga actagggttc
      721 cccgttgacg gctctgggcc atagccccat ggtccgcccc aaggaaatcg cccatgaacg
      781 aagccatgaa acccgcccca gcggccgaag tcgccatcgg aaatgtcagg ttcggcaatg
      841 acttgccgct ggcgctgatg gcgggacctt gcgtcttgga aagccgcgcc catgcgctgg
      901 agatggccag cgcgctgaaa gaaatcgccg caaaactcgg cgtcgggctc gtctacaagt
      961 cgtccttcga taaggcgaac cgcaccagcg cggcgggcgc ccgggggctc gggctcaagc
     1021 aagcactccc catttttgcc gaggttcgcg aggtgaccgg gcttcccgtc gtcacggacg
     1081 tgcacgagcc ggagcagtgt gcgcctgccg ctgaagcggc ggatgtcctg cagatcccgg
     1141 cctttctgtg ccgccaaacc gatcttctcg tggcggcggc gaagacgggc aaacccgtga
     1201 acgtcaagaa gggacaattc ttggcgcctt gggacatgcg gcacgtggtg gcgaaggtga
     1261 cgagtgccgg caatcccaac gtgcttgtca ccgagcgcgg cgtgtcgttt ggctacaaca
     1321 cgctcgtctc tgacatgcgc gcgctgccca tcctcgccga aaccggcgcg ccggtgattt
     1381 tcgacgcgac ccattcggtg cagcagcctg gcgggcaggg cgcctcgagt ggtggcgagc
     1441 ggcgtttcgt gccggtgctg tcgcgcgcgg ccacggccgt cggcgtgtcg gggctcttta
     1501 tcgagaccca ccaggacccg gacgcagcgc cgtcggacgg ccccaacatg gtgccgctga
     1561 aagagctgga gccgctgctt gccgaattga tagaaatcga ccgcttagtg aagtcccaag
     1621 cctaagacgt tttacgccgg cgcaatgaga cgcgccgctt caccatccca gcgccacaga
     1681 ttgtcttccg tgaggtgcgt gccgccccac cagtgatcca ctgcgcggtg cttggcccaa
     1741 tcggcgccgc ccgcaagcac cgccttgccg agcgatgtca ggctcaagcg gctggtgaga
     1801 taggcctttg caagttcggg atcttgatac tgaaacggcg cggactccaa tcccgcaatc
     1861 aacggttcgt cggcaagcgc aagctggtcc agcaatcgcc agaagctcca atcgcccatg
     1921 aactcggcgt cttccatctt ctgcaccgcg acgaaggctc cgagcgcggt cgcggactcg
     1981 tcggcttcga tcgctgcaag catctgccgc tctgtgcggg aaagaccatc cactcccgga
     2041 agttcctcca gcatccgcac gacagccgcg tgcaagaacg gaagcacgga gaggtcttca
     2101 tcgagcagcg cggcccacgc ttcgggagtc ggctgacgaa aagcggccca tgcgcgtttg
     2161 gccagatcga gctgcgcttg cgtgaccggc accgcagacg ccgcaagttg cggaatcgcg
     2221 tcaggttctt ggcggccgat atagtcctcg acctggacca gcgaaagcaa tccggcttga
     2281 ggcggatgat cggcgaacca atcaaggacc tgcaagagtt gcagctggtc gtataggtcg
     2341 tgctcaaacc acagcatgac gcgatcgaac tcagcgctaa tgccgagtcc gcggtcacgc
     2401 gccgtcatct cgaactctat ggtgccgaca tcacttccgg ccaaaccggc caagtattca
     2461 gcgcgggcct tggcaagagc ctctcgcgtg tcggtgagag gcaccggccc ttcgtgcaac
     2521 acgtcacgcc aaggcaagac ctcggcgccg atcagcgtct tgcgcaggag ctcgcccgca
     2581 acatcgccgt tggtgatgac caggtcggtc atccgatcaa ccgcgtcccc gataaggcgc
     2641 cacgccctgg tcggggatcc agacgccttc gggcggcgta ccatgctgcc aaaacacgtc
     2701 gatgggaatg ccgccgcgcg gataccaata accgccgatc ctgagccaag acggctcgat
     2761 caagtccacg atctgcttac cgatggtgag ggtcgcatct tcatggaacg cgctcgcatt
     2821 gcgaaagctg gctagaaaca gcttgaacga cttcgactcc aagagccatg cgccgggcgc
     2881 atagtcgatg acgaaatgtg cgaagtccgg ttgccccgtc accggacaaa ggcaggtgaa
     2941 ctccggcgcg gtgaaacgcg ccacataagc cgtgtccgca tgcgggttgg ggacgcgctc
     3001 cagcaccgcc tcttcaggcg agccaggaac tttcgtctct tgcccaagct gccgcaggcc
     3061 tgtgatcttg cgcgtcatcg tctactccgc agcgagccgc atgggcggtc catgataaac
     3121 gcagccctcg tggcaggaat cccgcgccac cacgatctcg accaggccgg gaagccggtt
     3181 gtgcagccgg tcccacagcc acatggcgat gcgctccagc gtgggatatt gcaggccctc
     3241 gacctcgttc agcatgcggt gatcgagcgc gtcgcgcgcc tccgccacgg cggcgtccaa
     3301 ctcatcgaaa tgcacgatga tgcccgtgtc ctcgtcgggc tcgccgtcca cggtaatgcg
     3361 cgcacggaag gagtgtccat gcacccgcga attcgggtga cccggtggcg ccgatggcaa
     3421 gaagtgcgcc gcctcgaaga agaattcttt gtagatacgc ataggggctg tttaggggat
     3481 ttcgcgtgac cggaccagag gttagagcag cctcgcgacg tttacggcag gcccagcaat
     3541 ttgtgggtct gcagactcaa gcgccatttg ggatgggcca ggcaataatc aatggccgcc
     3601 tgcagatgtt tgacgccgcc ggtgccatcg tccaacggtt gcagcaggta atggctgaaa
     3661 ttgagcgtct ggacctcctc cggccacagc ctgtcttgcg gatagaccag cttcagctca
     3721 tcgcctgagc gctgccggag cggcgcgccg ggcttcggac tgacgcagac ccaatcgatc
     3781 gaggtgagca cggggagggt gccgttcgtc tcgatggcca cctcaaagcc gactgtgtga
     3841 aaggcatcga ccaacgcctt atcgacctgc agcatcggct caccgccggt cagtacgacg
     3901 aagggcggcg tgcccgaatt gccgcgccag gtctcgcgac aagccaccgc caaggcatcg
     3961 gggcccgcga acttgccgcc gcccggacca tcggtcccaa caaagtccgt gtcgcagaaa
     4021 tcgcaaatcg cctgcgcccg atccacctcg cggcccgacc acagattgca gcccgcaaag
     4081 cggcagaaaa ccgccggccg cccggcctgg gcgccttcgc cttgcagcgt gtagtagatt
     4141 tccttgacgg tatacatctg gcgcggaatt tgacgccttc gcgtcccgcg agcaagtgcc
     4201 gaggtgccgc aacgtaaaat cgcccggaca tctgaatcga gcgcgatgcc cgggcgatcc
     4261 acctatccag agctactcgg cctttttctc gccgggcggc gtgtacttgc ccggaatgac
     4321 cttttccggc gggttggtga cgacagcggc tggccttctc gtggccggcc tttttattgt
     4381 cgggttcgcc gccgtcacga gcaagacgtc gcaacccacc tgaacgcagc ctgaaccaag
     4441 cgttgtctgg cggtcactta gcggctttgg ttgcgcgacc gctggaggag gcttgccatt
     4501 cccaatagcc cttggcgcgg agttcgcagg ccggacattc gccgcagcca tagccccaat
     4561 cgtaacgcgt gccgcgctcg cctttgtagc aggtgtgggt ttcctcgatg aggatatgaa
     4621 ccagcgcctc gccgccgacc tcttgggcga ggtcccaagt ctgtgccttc gacaaccaca
     4681 tcaatggcgt ctcgatgaca aacggtgcat ccgtcccgag cgcgatggat tcttgcaacg
     4741 cccgcaacgt cgcgtcgcgg cagtcgggat agccggaata gtccgtctcg cacatacccc
     4801 cgacaagcac attcaacccg cgccgataac cgagcgcggc ggcgtagacg aagaacgcga
     4861 gattgcggcc cggcacgaag gaggagggga gcccggagtc cagcatgacg atttcggcgt
     4921 cgctcgtcag ggccgtgtcg ccgatcgcgc caaagctcga aatatcgatc atatgatcgg
     4981 ggccgagccg cttcgcccaa tccggaaaat gccgttcgat cgccgcgcgg acccgaacgc
     5041 ggcactcgag ctcgacgccg tggcgctggc cgtagttgaa gccgacggtc tccacgcgtt
     5101 catagcgatc aagcgcttgg gcgaggcaaa cggtcgagtc ctggccgcct gagtgcagca
     5161 ccagcgcgct ttttttggcg tctgacgtct tttccggcat cgggtcgatc tctcgcggca
     5221 tgggagcgcg gttacatata cgccttccgc ttctccgccg cctaggctaa gacggcccaa
     5281 attctaaaag gggttcccgt atgactgcca tcgccaatat cgtcggccgt gaaatcctag
     5341 atagccgcgg caatccgacc gtcgaggtcg atgtcgtgct tgagggcggc catcatggcc
     5401 acgccgccgt cccgtccggc gcttcgaccg gcgcccacga ggcgcatgaa ctccgcgacg
     5461 ggggcacccg ctatggcggc aagggcgtgc agaaggcggt agaggccgtc aatggcgaga
     5521 tccgaaaagc cctcgatggc ttcgacgccg acgaccagcg cggcctcgat aagctgctct
     5581 gcgatctgga cggcaccgag aacaagaaac ggctcggcgc caacgccatt ctgggcgtct
     5641 cgctggcggc ggccaaggcg gcggcaagct ctcacgagca gccgctgtac cggtatctcg
     5701 ggggcgaaga ggcgaccttg ctgcccgtac cgatgatgaa catcgtcaat ggcggcatgc
     5761 atgccgacaa cccaatcgac tttcaggaat tcatgatcgt accggtcggc gcccccagca
     5821 ttgccgaggc ggtccgcatg ggcgcagaga ttttccatac gctgaaagcc gacctgaagg
     5881 cggccggcta cggcaccgcg gtcggagacg agggcggctt cgcgcctaat ctcgcctcga
     5941 cccgccaggg gctcgacttc gtcatggagt cgatcggcaa ggccggctac cacccgggca
     6001 aggacgtgta cctcgcgctc gatgtggccg cgaccgagtt ctacaaggac ggcagctacg
     6061 tgctcgctgg cgagaaccgg agcctcacgt cggacgagat ggcggcctat ctcgcagatc
     6121 tcgtggccaa ctatccaatc ttctccatcg aagacggcat gtccgaggac gattgggacg
     6181 gctggcgcag cttgaccgag atgcttgggt cccgggtgca gctcgtgggc gacgacctct
     6241 tcgtcaccaa tgtcacacgc ctccaggaag gcatcgaccg gaacatcgcc aactcgatcc
     6301 tcatcaaggt caatcagatc ggcacgctgt ccgaaacgct ggacgccgta aagatggccc
     6361 atgacgccgg ctatacagcg gtgatgtcgc atcgctccgg cgagaccgag gacaccacca
     6421 tcgccgacct ggccgtggcc accaattgcg ggcagatcaa gacaggctcg cttgcccgct
     6481 ccgaccgcgt ggcgaaatac aatcagctca tccgcatcga agaggagctc gggccccgcg
     6541 cgcgctacgc tggcgccgcc gttttgagaa cggcgtaaaa ctcgcctcgt ggtaaccgca
     6601 acttaacgcg tcccggccga tcttggtgat tcggtgtgtg gggaggggtc atgcggttcc
     6661 gggcgtttat ctttccgctg cttgcgttga gcgcaggcgg ctatttcgga tatcacctgc
     6721 agaatggcga ccacggcctg acgtcccatg ccgacctcga gcggcgtaag gacgtgctcg
     6781 aaggcgagct cgctggcttg cgcgaagtcc gtttgcgcat cgagcgtgac gttgcgctgc
     6841 ttgagcccga tagtctcgat ccggacatgc tggatgagcg cgcccgcgcc attctcaacc
     6901 tcgcccatcg cgacgatctc gtcatgatga agcggcgtgc gcccgatccc actgggccat
     6961 tgcgcaagcc ttgaccgctt aaggtctgat cgcctttttg cctgcttcga actcgtgcta
     7021 gctttttcga aggcgcgccg tattgagtcg aaggaggcca ggagtgaaca gcccaagcct
     7081 gacgccggga gcggccaact tctggatgga ccagaagcca ttcggaactc cggcttcagc
     7141 gccgccgaaa ggcgcacccc tcgcggtcga ggacgagctc aacgcctatc gcgacatgct
     7201 gctgatccgc cgcttcgagg agaaggcggg gcaactctac ggcatgggtt tcgtggccgg
     7261 cttctgccac ctctatatcg gccaagaggc cgtggtcacc ggcatgacga tggcgatcaa
     7321 gcctggcgac cagatcatca ccgcctatcg cgatcacggc cacatgctcg ccaccggcat
     7381 ggaccccaag ggcgtcatgg ccgagctcac gggccgcgcc catggctact ccaagggcaa
     7441 aggcggctcg atgcacatgt tctccgtgga gagaaacttc ttcggtggtc acggtatcgt
     7501 cggcacgccg gcgccactcg gcaccgggat cgcgttcgcc aataaatatc gcgacaacgg
     7561 caatgtctgc atggccttct tcggcgaagg cgcggccaat caaggccagg tctatgagag
     7621 cttcaacatg gcgtccctgt ggaagctgcc gatcgtctat gtcatcgaga acaaccacta
     7681 tgcgcttggc acatccgtcg agcgcgccag cgccgagtgg aaggagcttc atcatcgcgg
     7741 gctcgccttc ggcattcccg gcgaggaaat cgacggcatg gacgtggcga tggtgaaagc
     7801 ggcgggcgaa tgggcactcg cgcatgcccg cgaaggaaaa ggtcctgtca tcatcgaagc
     7861 cctgacctac cgctatcgtg gtcactcgat gtccgacccg gcaaagtacc gcacgcggga
     7921 agaagtgcag gatatgcgtc agcatcacga tccgatcgag ctggtgcgca aacgtctgct
     7981 cgatgcggga aaaagcgagg acgaattgaa agcgatcgac cgcgacatcc gcgccgtcgt
     8041 gaatgaggcg gcggagttcg cgcaaagcga tcccgaaccc gacccatccg agctctacac
     8101 cgacgtggtg gcgacgtgag cgcgaaccgc taatgtcgac agaaattctc atgcccgcgc
     8161 tttcgcccac tatggaggag ggcacgcttg ccaaatggca cgtgcgcgaa ggcgacgtag
     8221 tccatgcggg cgacgtcatc gccgagatcg agaccgacaa agccaccatg gaggtcgagg
     8281 cgatcgacga cggcacgctc gacaagatct tggtgcccga aggtgcggag catattccgg
     8341 tcaaccagcc catcgccatt ctgatgacag agggcgaaga cgacgtgtcc gccatcgagg
     8401 tgatgtcgca agcggcacct gctgacgctt ttcaggcccc gccaccg
//
LOCUS       DBNHVU010000012         4102 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_304361, whole genome shotgun sequence.
ACCESSION   DBNHVU010000012 DBNHVU010000000
VERSION     DBNHVU010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_304361"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="AC16Y7_00450"
     CDS             complement(<1..195)
                     /locus_tag="AC16Y7_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707880.1"
                     /translation="MANGVDASEHSDALVFFGATGDLAYKQIFPALYGLIRDEGLSLP
                     IIGVAKAGWGLDQLKGRAADS"
     gene            complement(195..1208)
                     /gene="gnd"
                     /locus_tag="AC16Y7_00455"
     CDS             complement(195..1208)
                     /gene="gnd"
                     /locus_tag="AC16Y7_00455"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011653425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HIR5707881.1"
                     /translation="MQLGMIGLGRMGANMVVRAMKAGHDCHVYDTHADAVKALKDKGA
                     KGSTDLGEFVKGLDKPRAVWMMVPAAVVDAVIAELAPLLDADDIIIDGGNSYYHDDLR
                     RAKALKEKNIHYVDVGVSGGVWGLERGYCMMIGGEDKVVAHLDPVFRTLAPGVDEAPR
                     TPGAKGEASPAENGYLHCGPNGAGHFVKMVHNGIEYGVMAAYAEGLNILKHGNAGKVK
                     RDTDAETTPLRNPEYYQYDINLPEVAEVWRRGSVIGSWLLDLTAAALRHSPDLAEFEG
                     RVSDSGEGRWTLQAAIDEGVPAPVISSALFTRFASRGNAEFADKLMSAMRFEFGGHHE
                     KKA"
     gene            complement(1269..3341)
                     /gene="tkt"
                     /locus_tag="AC16Y7_00460"
     CDS             complement(1269..3341)
                     /gene="tkt"
                     /locus_tag="AC16Y7_00460"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR5707882.1"
                     /translation="MAQTPDKIDELSINTIRTLSIDAIQKANSGHPGTAMDAAPTAYT
                     LWQQFLRYDPKAPHWINRDRFVLSAGHASMLLYSLIYLAGIEAAEGSYQKKGGEAVSL
                     KDIEGFREDGSRCPGHPEYGWTTGVESTTGPLGQGIATSVGMAIAGKWLAETYNKPDF
                     DLFDFNVYALHGEGCLMEGVGSEAASLAGHLKLSNLCWIFDNNNVTIDGHTDITFTED
                     VAKRFDAYGWNVVRVSNANDLDELAAAYKDFLATDDKPTMIIVHSHIGYGSPDKHDTP
                     AAHGSPLGPDEVRRTKEFLGFDPDKFFVVPDGVRQHFTTHMGQRGATARAKWEALFAD
                     YAKKHPDEAKQIEQIRDRELPDDWDADLPEFALDPRGIATREASGKTLNAIAAKVPWV
                     VGGAADVAGSTKVLLTYPDANEFQPYGELGDYSGHNLRYGIREHAMCALVNGAVLTGM
                     RAYDSCYLIFTDYARGAIRLASLMDLPVLHIWTHDSISLGQDGPTHQPVEQIVSLRAI
                     PGLMVVRPADANETVEAYRIAMPHKSRPVALILTRQAVPTFDRSKLAPASGLAKGAYV
                     LADADDGKPDVILIGTGSEVSLCLAARDLLAKDKIKARVVSMPCRELFECQTQAYRDS
                     VLPPQITARVTVEEASPIGWDRYAGPKGIVLGMDTFGLSAPLKVVSEHFGFTPERVAA
                     AAKKVLDS"
     gene            3565..3783
                     /locus_tag="AC16Y7_00465"
     CDS             3565..3783
                     /locus_tag="AC16Y7_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HIR5707883.1"
                     /translation="MAETGASTKDQSDLATLPLDKLEAKLGTSPEKGLTVAEAEKRLA
                     QYGANEIAEKKISLLLKFLSYFWGPSRG"
     gene            3780..>4102
                     /locus_tag="AC16Y7_00470"
     CDS             3780..>4102
                     /locus_tag="AC16Y7_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707884.1"
                     /translation="MIEAAAILSAVVHHWSDFVIIMVLLLANAIVGFWKEDQAGYAIA
                     ALKAKLAPHARVKRGGAWSDLAAKLLVPGAAAAFRNVSASRGSKRFRGLEMDGFVTKF
                     SGLAGA"
BASE COUNT          710 a         1252 c         1320 g          820 t
ORIGIN      
        1 gctgtccgcc gcgcggcctt tcagctgatc cagcccccag cccgccttcg cgacgccgat
       61 gatgggcaag gacaagcctt cgtcgcgaat gagcccgtag agcgcgggga agatctgctt
      121 gtacgcgaga tctccggtgg cgccgaagaa gacgagcgcg tcggaatgct ctgaagcgtc
      181 tacgccgttc gccattacgc tttcttctcg tgatgaccgc caaactcaaa ccgcatggcc
      241 gacatcagct tgtcggcgaa ctccgcattg ccgcgggagg cgaagcgcgt gaacagtgcc
      301 gaagagataa ccggagccgg cacgccttcg tcgatcgccg cttgcagggt ccagcggcct
      361 tcgccggaat ccgatacgcg gccttcgaac tcggctaagt cgggactgtg acgcagcgcc
      421 gccgcagtga ggtcgagcag ccaagagccg atcacgctgc cgcgccgcca cacctcggcg
      481 acttccggca ggttgatgtc atattgatag tactcggggt tgcgtaaggg tgtggtctcc
      541 gcgtccgtgt cgcgcttcac cttgccggcg ttgccgtgct tcaagatgtt caggccttcg
      601 gcgtaagcgg ccatgacgcc gtactcgatg ccgttgtgga ccattttgac gaagtgcccg
      661 gcgccgttcg gcccgcaatg gaggtagccg ttctccgccg gcgatgcttc gcccttcgcg
      721 cccggcgtgc gcggggcttc atcgacgccc ggcgccagtg tgcggaagac cggatcgaga
      781 tgcgccacca ccttgtcttc gccgccgatc atcatgcagt agccgcgttc gaggccccag
      841 acgccgccgc tgacgccgac atccacatag tggatgttct tttctttcag ggctttagcg
      901 cggcgcaggt cgtcgtgata gtaggagttg ccgccatcga taataatgtc gtcggcgtcc
      961 agcagcggtg cgagttccgc aatcaccgca tcgactacgg cggcgggcac catcatccaa
     1021 accgcgcgcg gcttatccaa acccttgacg aattcgccga ggtcggtgct gcccttcgcg
     1081 cccttgtcct tgagcgcctt caccgcatcg gcgtgcgtgt cgtagacgtg acagtcgtgg
     1141 ccggccttca tcgcccgcac caccatgttg gcgcccatcc gaccgaggcc gatcatgccg
     1201 agttgcatcg cttctctcct cgtttcgcaa atccaagaac gctccggggt cccggagcgc
     1261 gggaacgtct aactgtccaa aacctttttg gccgccgcgg cgacccgctc cggtgtgaac
     1321 ccgaaatgct ccgagacgac tttcagcggc gccgacaggc cgaacgtgtc catgccaagc
     1381 acgatgccct tcggccccgc atagcggtcc cagccgatcg gggaggcttc ttccaccgtg
     1441 acgcgcgcgg tgatctgtgg cggcagcacg gagtcgcgat aggcttgggt ttgacactcg
     1501 aacagttcgc ggcaaggcat tgaaacgact cgcgccttga tcttgtcctt ggcgagcaga
     1561 tcgcgcgcgg caaggcacag cgacacctcg ctgcccgtgc cgatgagaat gacatcgggc
     1621 ttgccgtcgt ccgcgtcggc gagcacataa gcgcctttgg cgagaccgga agccggcgca
     1681 agtttcgagc ggtcgaacgt ggggaccgct tggcgcgtca ggatcagcgc cacgggccgg
     1741 ctcttatgcg gcatggcgat gcgataggct tccaccgtct cgttggcgtc cgccggacgc
     1801 acgaccatga gaccggggat cgcacgcaac gatacgatct gctcgacagg ctgatgggtc
     1861 gggccgtcct ggcctaagct gatggaatcg tgcgtccaaa tgtgcagtac cggcagatcc
     1921 ataagcgagg ccagccggat tgcgccgcgc gcatagtccg tgaagatcag gtagcaggag
     1981 tcgtaggcgc gcatgcctgt cagcaccgcg ccgttgacga gcgcacacat ggcgtgttcg
     2041 cggatgccgt agcgcagatt gtggccgcta tagtcgccga gttcgccgta aggctggaac
     2101 tcgtttgcgt ccgggtaggt caaaagcacc ttggtactgc cggccacgtc cgcggcgcca
     2161 ccaacgaccc acggcacctt cgccgcgatg gcgttcagcg tcttgccgga ggcttcgcgc
     2221 gtagcgatcc ccctggggtc gagcgcaaat tccggaaggt ctgcatccca atcgtcgggc
     2281 agttcgcgat cgcggatctg ctcgatctgt ttggcctcgt ccggatgctt cttggcgtag
     2341 tcggcgaaca gggcttccca tttggcgcgg gcagtggcgc cgcgctgacc catatgcgtg
     2401 gtgaagtgct gacgcacacc atccggcacc acgaaaaact tatcgggatc gaagcccagg
     2461 aactccttgg tgcggcgcac ctcgtcgggc ccaagcgggg agccatgtgc ggcgggcgtg
     2521 tcgtgcttgt cgggcgagcc gtagccgata tggctgtgca cgatgatcat cgtcggcttg
     2581 tcgtcggtgg cgagaaagtc tttgtatgcg gctgccaact cgtcgagatc gttggcgttc
     2641 gacacgcgga cgacgttcca gccataggcg tcgaagcgct tggccacgtc ctcggtgaag
     2701 gtgatgtcgg tgtggccgtc gatagtcacg ttgttgttgt cgaaaatcca gcacaggttc
     2761 gacagcttca ggtggcctgc gagcgatgcc gcctcgctgc cgacgccttc catcaggcag
     2821 ccttcgccgt ggagcgcata gacattgaaa tcgaagagat cgaagtccgg cttgttgtag
     2881 gtctcggcga gccacttgcc ggcaatcgcc atgccgacgc tggtcgcgat gccttggccg
     2941 agcggcccag ttgtggattc aacgcccgtg gtccagccat actcgggatg cccggggcag
     3001 cggctgccgt cctcgcggaa accctcgatg tccttgagac ttaccgcctc gccgcccttt
     3061 ttttggtagg agccttcagc cgcttcgata cctgcgaggt agatgaggct gtagagcagc
     3121 atcgaggcgt ggcccgccga aagcacgaac cggtctcggt tgatccagtg cggcgccttc
     3181 gggtcgtagc ggaggaattg ctgccacagc gtgtaggcgg tcggcgcggc gtccatggcc
     3241 gtgccgggat gacctgaatt ggccttctga atggcgtcga tggagagcgt gcggatcgtg
     3301 ttgattgaaa gttcgtcgat cttgtctggg gtctgcgcca ccgtcgccgt tcctcccttt
     3361 gtctcagggg cgctgaattc aatacagcat gcggcattga gatcaaagcg tcgagccgca
     3421 tggttacttt ggcgagggta gtaaactgct tgtcacagtc ttgacgtctt atccacggga
     3481 cgcgcgagac ttggggcggc cgttgggggg catccttgcc gcgccgcgat gccttggttg
     3541 aaagaagacg aggagagcac gcccttggct gagaccggag cttcgaccaa agatcagtcg
     3601 gatctcgcga cccttcccct cgataagctc gaagccaagc tcggcactag ccccgagaaa
     3661 gggctcacgg ttgcggaggc cgagaagcgc ctcgcgcaat atggcgccaa cgagattgcc
     3721 gagaagaaga tcagtctgct cctcaagttt ctctcgtatt tctggggccc atcccgtgga
     3781 tgatcgaggc ggcggcgatt ttatcggctg tcgtgcacca ttggtccgac tttgtaatca
     3841 tcatggtgtt gctcctcgcc aacgccatcg tcggattctg gaaggaagat caagccggct
     3901 acgccattgc cgcactgaag gccaagctcg cgccgcatgc gcgggtgaag cggggtgggg
     3961 cctggtcgga ccttgccgca aagctcttgg tgcccggcgc ggcggctgcg tttcggaatg
     4021 tttcggcttc gagagggtcg aaacgtttcc ggggtttgga aatggacggt ttcgttacaa
     4081 aattctcggg attggcaggg gc
//
LOCUS       DBNHVU010000013         4662 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_750522, whole genome shotgun sequence.
ACCESSION   DBNHVU010000013 DBNHVU010000000
VERSION     DBNHVU010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_750522"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="AC16Y7_00475"
     CDS             <1..704
                     /locus_tag="AC16Y7_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HIR5707885.1"
                     /translation="DTVKEVIAFLGQQPGIAGVDMLTQEQSAELLEPWLGDVSNIKEL
                     PIPRLLAIEIDSDRTPNFTAIRSELASRFQGVTLDDHRQWQQQIRTVTRSFALGGLAV
                     LALVAAATTAIIITATRSAMSSNREIVEVLHFVGATDRFIAHEFERNFLRLGVRSGIL
                     GALVAALIFLALPMILHFLGGGAMATTEMQRFLGSGTLDLKGYGFLALVILVVAALCM
                     LTSRVSVFRILNSRD"
     gene            1042..1587
                     /locus_tag="AC16Y7_00480"
     CDS             1042..1587
                     /locus_tag="AC16Y7_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR5707886.1"
                     /translation="MRADAIVVLTGGGARIAAAGKLLREGRANRLLISGVNTSTRKKD
                     LLRLSRLPPEKFDCCVDVGYEALNTAGNASETRSWVRDNNFISVIVVTSGYHMPRSLM
                     ELKREMPGITLIPYPVQPTSLSRERWWLDKTTMRVLVSEYLKLLPAAARFAASRIVGN
                     LDKSTAATKSGQSRAESRADM"
     gene            1623..2384
                     /locus_tag="AC16Y7_00485"
     CDS             1623..2384
                     /locus_tag="AC16Y7_00485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR5707887.1"
                     /translation="MLRSLLFALAFYLTTALYLLFGSWLLLAPRTWAMAGLKSHGRTC
                     VKLLKWIVGTDIDVRGLDKVPPGGALIVSKHQSAWDTFALVPLFRDPAIVLKDELKWI
                     PVYGWFCIKFGHILVKRDKRAAALKNMLAQAREKIAAGRDILIFPEGTRQPPGAPPAY
                     MPGYLALYEGLNVPCVPLALNSGPFWPRRKLIRYPGTIVVEFLDPIPPGLDRKAFRRR
                     LENDLEAASHRLLAEAANAPNAPPTAAAFANRNKN"
     gene            2479..>4662
                     /locus_tag="AC16Y7_00490"
     CDS             2479..>4662
                     /locus_tag="AC16Y7_00490"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HIR5707888.1"
                     /translation="MSKDPDFLAPIAERIWRQKYQFRPASEQGGDARGGDIALADTFN
                     RVATAAASAEPGGAKAKQKWMRRFYDSMADFGFIPAGRILAGAGTGREVTLFNCFVMD
                     TIGDDLSDIFDNVREAALTLQQGGGIGHDFSTLRPKGALVQSVGADASGPVSFMDVWD
                     AMCRTIMSAGTRRGAMMATLRCDHPDIEAFIDAKKDPARLRNFNLSVLVTDAFMDAVQ
                     KDAPWPLIFNGKVYRTVEARSLWNRIMRATYDYAEPGVIFIDRVNRRNNLAYCETISA
                     TNPCGEQPLPPYGACLLGSINLTAFVKDAFTTSAALDLKAIKRRVRVATRLLDNVIDI
                     SNYPLTAQHQEALAKRRIGLGLTGLADTLAVLGIRYGSNEAAQHAANWMQTIQNAAYQ
                     ASARLAKRKGAFPLYDEKAMFSQPNLAALDPKTRKLIRRHGLRNGCLTSIAPTGTISL
                     LAGNVSSGVEPIYDYRYKRRVLKADGTRDDLVVEDYAVAAYRRRFGADADLPPAFTTA
                     RDLTPFEHLKMQAALQPHVDSSISKTINCPEHLPFDDFKSIYAHAYSLGLKGCTTYRP
                     NPVTGAVLETVDAGQAQHAPQQGSAPVPDPEAILSAEHQPRPALDIVPPAGANLARPA
                     GLPAASPAAGLTPPAFDAQADANTPPGDVIYVSKPLARDTVLTGATYKLKWPESDNAL
                     YITINDVEVGGRKRPFEIFINTRHLEHYAWTVALTRMISAVFRRGG"
BASE COUNT          949 a         1598 c         1222 g          893 t
ORIGIN      
        1 aagacaccgt caaggaggtc atcgccttcc ttggacagca acccgggatt gccggtgtcg
       61 atatgttaac gcaggagcaa agcgccgaac tcctcgagcc ctggctcggc gatgtcagca
      121 acatcaagga gctgcccatc ccgcgcctgc tcgccatcga gatcgacagc gacaggacac
      181 cgaacttcac cgccatacgt tccgaactcg catcgcgctt tcaaggtgta acgcttgacg
      241 atcaccgcca atggcagcag cagatccgca ctgtgacccg cagctttgcg ctcggcggtc
      301 tcgctgtcct ggccctcgtc gccgctgcca cgaccgccat catcatcacc gccacccgat
      361 cggcaatgtc ctctaatcgc gaaatcgtcg aagtgctcca tttcgtcggc gccaccgacc
      421 gcttcatcgc tcacgaattc gaacggaact tccttcgcct tggcgttcgc agcggtatcc
      481 tgggcgccct ggtcgccgca ctgatcttcc tggccctgcc catgatcctg cattttctcg
      541 gtggcggtgc catggccaca acggagatgc agcgcttcct cggctccggc accctcgacc
      601 tcaagggcta tggatttctt gccctcgtca tactggtcgt tgcagcgctc tgtatgctga
      661 cgtcaagggt gagcgtgttc cggattctca acagccgcga ctagccgttg tccgacattc
      721 ctgctttact ttgaaaagat agacagcccc tggcagtcgc cagtctggtg tatttgtagg
      781 gaactggctt gtgcgattgc caggttgcgg ccgctgtctt gttcggtggg gcctttggcc
      841 gtcagatgaa gaccagtgca tgcagagacg gcagccggtg aaggctgcct tccgaagtct
      901 tgccgcgcgg cggaggcgcg caccgttcca cacatggcaa agcgactcat cataggcgtt
      961 ttagggttgt ccgcctcgct gctcgcgttc gggtttattg cattctcgat cgtcgctttg
     1021 cggtctgcag gcactgaaaa cgtgcgcgct gacgccatcg tggtgttgac gggcggcggc
     1081 gcccgcatcg cagccgcagg caagttgctt agggaaggcc gcgccaaccg gctcttaatt
     1141 tccggcgtca acacgtcgac gcgcaagaag gaccttctgc gcctgtcgag actgccgcct
     1201 gaaaaattcg actgctgcgt cgacgttggc tacgaggcac tcaacaccgc aggcaatgcc
     1261 agcgagacca gatcatgggt gcgcgacaat aacttcatta gcgtaatcgt cgtcacgtca
     1321 ggctaccaca tgccaagaag cctgatggag ctgaagcgcg aaatgccagg catcaccttg
     1381 atcccttatc ccgtgcagcc aacgtcgcta agccgcgaac gatggtggct cgataagacg
     1441 acgatgcgcg tcctggtttc cgagtacttg aaacttcttc ccgctgctgc tcgctttgca
     1501 gcctcgcgta tcgtcggaaa tttggataag agtaccgcag ccaccaagtc gggccaatcg
     1561 cgcgctgagt ctcgcgccga catgtaacgg ccaaaaaaac gttcaggcat taacaagaca
     1621 ccatgctgcg ctccctcctg ttcgccctcg ccttctatct aacgacggcg ctttatctcc
     1681 tgttcggctc ctggctcctg ctcgcacccc gcacctgggc gatggcgggg ctgaaaagcc
     1741 atggccgcac ctgcgtcaaa ctgctgaagt ggattgtcgg cacagacatc gacgtccgcg
     1801 gtctcgacaa agtcccgccc ggcggcgccc tcatcgtctc aaagcatcaa tccgcctggg
     1861 atacgttcgc cctggttccg ctgttccgcg atcccgcaat cgtcctcaaa gacgaactca
     1921 aatggattcc cgtttacggc tggttctgca tcaagttcgg ccacatcctg gtcaagcgcg
     1981 acaagcgggc agcagccttg aagaacatgc tcgcacaggc ccgcgaaaaa atcgcagccg
     2041 gccgcgacat cctcatcttt cccgaaggga cccgccagcc gcccggtgcc ccgccagcct
     2101 acatgcccgg ctacctcgcg ctttacgaag gcctcaacgt gccgtgtgtt ccgctggccc
     2161 tgaactcagg ccccttttgg ccgcggcgca aactcatccg ctatcccggc acgattgtcg
     2221 tagagttcct cgatcccatt ccccccggcc tcgaccgcaa agcgttcagg cgcaggcttg
     2281 aaaatgacct cgaggccgcc agccaccgcc tcctcgccga agccgccaat gcacccaacg
     2341 ccccaccaac cgcggcggca tttgcaaata ggaacaaaaa ttaaacaaaa gaagaacatt
     2401 tgttgacgag gccggccttg gcccctattt tgtaaagcac cccaatccct gcgtcgtagc
     2461 tgctcatagt tctgcgtcat gtcgaaagac cccgattttc tggccccaat cgccgagcgc
     2521 atctggcgcc agaagtacca attcagaccc gcctcagaac aaggcgggga cgcgcgaggc
     2581 ggcgatatcg ctctcgccga caccttcaac cgtgttgcaa ccgccgccgc ttcagccgag
     2641 cctggaggcg ccaaagccaa gcagaaatgg atgcggcgct tttatgactc catggccgac
     2701 ttcggcttca tcccagccgg gcgcattctg gctggtgccg ggaccgggcg cgaggtcacg
     2761 ctcttcaact gctttgtcat ggacaccatc ggcgacgacc tctccgacat cttcgacaac
     2821 gtccgcgaag ccgcattgac gctgcagcaa ggcggcggga tcggccacga cttttccacg
     2881 ctgcggccca aaggcgccct cgtccaaagc gtcggcgccg atgcctctgg tcccgtcagc
     2941 ttcatggacg tctgggatgc catgtgccgg accatcatgt ccgcaggcac acggcgcggc
     3001 gccatgatgg caacgctcag atgcgaccac cccgatatcg aagcctttat cgacgccaag
     3061 aaggacccgg cccggctcag gaatttcaat ctctcggtcc tggtcaccga cgccttcatg
     3121 gacgccgtgc aaaaagatgc gccgtggccg ctcatcttca atggcaaggt ttaccgcact
     3181 gtcgaagccc gctccctatg gaaccgcatc atgcgcgcga cgtatgacta cgctgagcca
     3241 ggtgtcatct tcatcgaccg cgtcaaccgg cgcaacaatc tcgcatattg cgaaacgatt
     3301 tcggccacca atccctgcgg tgaacaacca ctccctccct atggcgcctg cctgcttggg
     3361 tcgatcaacc tgacggcttt cgtcaaagac gcattcacca cctctgccgc gcttgacctc
     3421 aaggcgatca agcgccgtgt ccgcgtcgcc acccgcctcc tcgacaacgt catcgacatt
     3481 tcgaactacc ccctgacagc ccagcaccag gaagcgctgg ctaagcgccg catcggactc
     3541 gggctgactg gtctcgccga taccctagcc gtgctcggca tccgttatgg gtctaacgaa
     3601 gcagcacaac acgccgcgaa ctggatgcaa acaatccaaa acgccgccta ccaggccagt
     3661 gccaggctgg ccaagaggaa gggggccttc ccgctttacg atgaaaaggc aatgttttct
     3721 cagcccaacc tggcagcatt ggatcctaaa acgcgaaaac ttattcgccg ccatggcctg
     3781 cgcaacggct gcctgacatc gatagcgccg accggcacca tttcgcttct tgctggcaac
     3841 gtctcaagtg gcgtcgaacc gatttacgat tatcgctaca agcgaagagt cctgaaagcc
     3901 gacggaaccc gcgacgacct cgtcgtcgag gactatgccg tcgccgccta ccgccgccgt
     3961 ttcggcgccg acgccgatct gccgccggcc ttcacgaccg cccgcgacct gacacccttc
     4021 gaacatctga agatgcaggc cgccctccag ccgcacgtcg acagctcgat ttcaaaaacc
     4081 atcaactgcc ccgaacacct cccctttgac gacttcaagt cgatttatgc ccatgcttac
     4141 agcctcggcc tcaagggttg cacgacatac cggccgaacc cggtcacggg agccgttctc
     4201 gaaactgtcg atgccgggca agcccaacac gcgccgcagc aaggcagcgc cccggtgccc
     4261 gacccagaag caatcctgtc tgctgagcac cagcctcggc cggccctgga catcgttcca
     4321 ccggcaggcg caaatttggc gcgaccagca ggcctcccgg cagcatcgcc agccgccggt
     4381 ctcaccccac cggcctttga tgcccaggct gatgcaaaca cgccacccgg cgatgtcatt
     4441 tatgtctcaa aacctctcgc ccgcgacacc gtcctcactg gcgccaccta caaattgaaa
     4501 tggcccgaga gcgacaatgc gctttacatc accatcaacg atgtcgaagt cggcggccgc
     4561 aaacgcccat ttgagatttt catcaacacc cgccatctcg aacattacgc ctggacagtc
     4621 gcgttaacac gcatgatcag cgcagtattc cgccgcggcg gc
//
LOCUS       DBNHVU010000014         6129 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_527441, whole genome shotgun sequence.
ACCESSION   DBNHVU010000014 DBNHVU010000000
VERSION     DBNHVU010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6129)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6129)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6129
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_527441"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="AC16Y7_00495"
     CDS             complement(<1..1210)
                     /locus_tag="AC16Y7_00495"
                     /inference="COORDINATES: protein motif:HMM:NF040455.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase scaffold domain-containing
                     protein"
                     /protein_id="HIR5707889.1"
                     /translation="MRAIALGIALAVGVSLPGLAAEPDGPAELIGKTEAIRIAIQDQV
                     GANSMPDVAEQIALAEYYSVSGRKPLWVDENGLTKRGKAVMAEIAKADDYGLRTSDYK
                     LPKLSGFNPAAPNATETLADAELKISAAAIEYASDARGGRITPQSLSKNLDPSLFLPK
                     PAEVLAFIAIRSDPAAYLRSFQPTHPQFEALRKELIAERGGNAPQQQEVSGPEDVVIP
                     AGPALKLGVEHGQVALLRKRLEVPVKDGANEYVFDAELREAVKAFQRTQGGKADGIVG
                     RGTRRLLNGNEAAAPQVSGQQKIDLLLVNMERYRWLPQDLGSFYVKVNIPAFKVSVKG
                     DEGTVWESRVVVGKTHTQTPIFNDEMEEIVFRPNWYVPNSIKTNEIAPYLRRGGGFFS
                     GGWDTSVLKRQ"
     gene            complement(1378..1767)
                     /locus_tag="AC16Y7_00500"
     CDS             complement(1378..1767)
                     /locus_tag="AC16Y7_00500"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR5707890.1"
                     /translation="MNRDEIEAFVLGLYDARVTGDMDVLAGIFADDATFQIAGSPGHS
                     LLATVAEGHEGVMALLQTIVDTLELENLTILDLLVEDNKVAVRWRATVHQVTSGEIHT
                     SELADFIEIRNGKVVSFIEFLDTALAG"
     gene            complement(1780..2391)
                     /locus_tag="AC16Y7_00505"
     CDS             complement(1780..2391)
                     /locus_tag="AC16Y7_00505"
                     /inference="COORDINATES: protein motif:HMM:NF021453.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2165 family protein"
                     /protein_id="HIR5707891.1"
                     /translation="MLITRYAKIVMVLSLAIFCLLVAFTNLTDYSTNFLFVKHVLSMD
                     TTFPGNALMYRSITSPELWSAAFGLIIASQIVAGVLFLAGAICLWQERHASGLAFNGA
                     KGLTIAGALAAFLIWFFGFMVIGGEWFAMWQSQTWNGQDAAFRFYVTALAVLIFVNQA
                     DGDLPAPAKAPQAKRTAPKKAASKTRNPTRRRTDKGRRRTDTA"
     gene            complement(2532..3551)
                     /gene="ilvC"
                     /locus_tag="AC16Y7_00510"
     CDS             complement(2532..3551)
                     /gene="ilvC"
                     /locus_tag="AC16Y7_00510"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR5707892.1"
                     /translation="MRVYYDRDADLNLIKGKKVAIIGYGSQGHAHALNLKESGVKEVA
                     VGLREGSSSAAKAEGEGLKVMSVADAAKWADVVMMLTPDELQAGIWENDLKPNMKQGA
                     ALLFAHGLNIHFNLIEPRADMDVCMVAPKGPGHTVRAEYKRGAGVPCLVAIAQDATGN
                     AHDLSLSYASAIGGGRAGIIETTFREECETDLFGEQAVLCGGLVELMRNGFETLVEAG
                     YAPEMAYFECVHEVKLIVDLIYEGGIANMNYSVSNTAEYGEYVSGPRVVNEQSKAAMK
                     DVLSDIQSGKFVKDWMLENKINQPSFKAMRAKCDDHDMEVVGKKLRDMMPWIAERKMV
                     DKAKN"
     gene            complement(3633..4175)
                     /gene="ilvN"
                     /locus_tag="AC16Y7_00515"
     CDS             complement(3633..4175)
                     /gene="ilvN"
                     /locus_tag="AC16Y7_00515"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR5707893.1"
                     /translation="MTQPGSAYSLDKQEQNPESHTISVLVDNEPGVLARVIGLFSGRG
                     YNIESLTVSETEHEAHLSRITIVTTGTPMVIEQIKHQLDRLVPIHSVTDLTVLGQPIE
                     RELALLKVAGTGEKRVEALGLAEAFHAEIIDSSTEHFVFEITGEQSRIEEFIALMQPL
                     GLVEVCRTGLAAIARGAERM"
     gene            complement(4175..5941)
                     /locus_tag="AC16Y7_00520"
     CDS             complement(4175..5941)
                     /locus_tag="AC16Y7_00520"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HIR5707894.1"
                     /translation="MSRTMTGAQIVLEALADQGVEHIFGYPGGAVLPIYDELFQQDRL
                     KHVLVRHEQGAVHAAEGYARSTGKVGVVLVTSGPGATNAVTGLTDALMDSIPVVCITG
                     QVPTHLIGNDAFQECDTVGITRPCTKHNYLVKDVDQLSTVLHEAFYVARSGRPGPVVV
                     DIPKDIQFASGLYAGLSNSQHKSYRPPKEGDSAAVEAAVEAMATATRPIFYTGGGVIN
                     AGPAASERLRELVHMTGYPITSTLMGLGAFPASDKQWLGMLGMHGTYEANNAMHDCDV
                     MICIGARFDDRITGRVDAFSPGSKKIHVDIDPSSINKNVQVDIPIVGDCDHVLNQMIA
                     AWKKKKPKVDKPALKTWWDQIDTWRARKCLHYKNRDDIIMPQYAVQRLFELTRGKDVY
                     VTTEVGQHQMWAAQHFHFDEPNRWMTSGGLGTMGYGIPAAVGVQMAHPDSLVIDIAGE
                     ASVLMTMQEMSTAVQYDLPIKIFILNNEYMGMVRQWQELLHGGRYSHSYTEALPDFVK
                     LAEAYQAVGIRAEKPSELDDAIREMIETPRPVIFDCRVHKHANCFPMIPSGEAHNNML
                     MGDDVTEEDIENAITDEGKVMV"
BASE COUNT         1110 a         1915 c         1891 g         1213 t
ORIGIN      
        1 cctgacgctt caggacggag gtatcccatc caccgctaaa aaagccgccg ccacgccgga
       61 ggtatggcgc gatctcattg gtcttaatgg agttcggcac ataccaattt gggcggaaga
      121 cgatttcttc catttcatcg ttgaagattg gcgtctgggt gtgcgtcttg ccgacgacca
      181 cgcgggattc ccagaccgtg ccttcatcgc ccttcacact gaccttaaac gccgggatgt
      241 tcaccttgac gtaaaagctg cccaggtcct gcggtagcca gcgatagcgc tccatgttga
      301 cgaggagcag atcgatcttc tgctgcccgc tgacctgcgg cgccgccgcc tcatttccgt
      361 tcaacaagcg ccgcgtgcca cggccgacga taccgtcggc cttgccgcct tgggtgcgct
      421 gaaaggcttt aaccgcttca cgcagctccg cgtcgaagac gtattcgttg gcaccgtcct
      481 tcacgggcac ttcgagccgc ttcctaagca gagcgacctg cccgtgctcg acacccagct
      541 tcagggccgg gcctgcggga atgacaacat cctcgggacc cgacacctct tgttgctggg
      601 gggcgttccc cccacgctcg gcaatgagct ctttacggag agcctcgaat tgcgggtggg
      661 tcggctggaa actccgcaag taggcagcag gatcggagcg aatggcaatg aaggcgagca
      721 cttcggccgg cttcggcagg aagaggctgg gatccaggtt cttgctgagg ctttgcggag
      781 tgatgcgccc gccccgggca tcgctggcgt actcgatcgc cgccgcgctg atcttcagct
      841 ccgcatccgc caaagtctct gtggcgttgg gggcggcagg attgaacccg gagagcttcg
      901 gcaatttata gtcggaggtg cgcaggccat agtcgtcggc tttggcgatc tccgccatga
      961 ccgccttgcc gcgcttggtg aggccgttct cgtcgaccca gagcggcttg cggcccgaaa
     1021 cggaatagta ttcggcgagc gcgatctgct cggcgacgtc gggcatggag ttcgcgccaa
     1081 cttggtcctg gatggcgatc cggatcgcct ccgtcttgcc gattagctcg gccggaccat
     1141 cgggctcggc tgccagacct ggaaggctga ccccgaccgc cagggcgatt cccaaggcga
     1201 tggctctcat aaaatctgct ccctctagcc ccgcgcttga ggacgaatcc gtccttttcc
     1261 cgttcagttt ggcggattat gcgccaaccg ctattagcag accacccaaa tccggccctg
     1321 aaatggcaag tctcacagga ttgtgcaaga ccccaggcga ccaaaaaact cccggcccta
     1381 tccggcgagc gccgtgtcca ggaactcgat gaaggagacc accttcccgt tccggatttc
     1441 aatgaagtcg gccagttcgc tggtatggat ctcgccggac gtaacctggt ggacggtagc
     1501 acgccagcgc actgcaacct tgttgtcctc aacgaggagg tcgagaatgg ttaagttctc
     1561 taattcgagc gtatccacaa tggtctggag cagcgccatc accccttcgt ggccctcggc
     1621 gacggtggcc aacaggctgt gccccggcga gcccgcgatc tggaaggtgg cgtcgtcggc
     1681 gaatatcccg gcgagcacgt ccatgtcgcc tgtcacccgg gcgtcgtaga ggcccaaaac
     1741 gaaggcctcg atctcatcgc ggttcatctc gaagtctccc tacgcggtgt cggtgcggcg
     1801 gcgccctttg tcggtgcggc ggcgggtggg gttacgcgtc ttggacgcag ccttcttggg
     1861 cgccgtcctc ttggcctggg gcgctttggc cggcgcgggc agatcgccgt cggcctggtt
     1921 cacgaagatc agcacggcca gcgcggtgac atagaagcgg aaggctgcat cctggccgtt
     1981 ccaggtttgc gactgccaca tggcgaacca ctccccgccg atcaccatga agccgaagaa
     2041 ccagatgagg aaggcggcga gcgcgccggc gatcgtgagc cccttggcgc cattgaacgc
     2101 gagccccgaa gcatgccgct cctgccacag gcaaatggcg ccggccagga acaggacacc
     2161 cgccacgatc tggctggcga tgatcaaccc aaaggcggcg ctccacagct cgggactggt
     2221 gatcgaccgg tacatcaacg cattgcctgg aaacgtcgtg tccatgctga gcacgtgctt
     2281 cacgaacagg aagttcgtgc tgtagtcggt caggttggtg aacgcgacga gcaggcagaa
     2341 gatcgccagg ctcagcacca tcacgatctt ggcgtagcgg gtgatcagca tggcggttcc
     2401 cctctcccgg cgtcacggat cacatcatag atcgacccta ccggcgactg gattcggatt
     2461 tgatgacacc ggaaagacga acggccgggg attcccggcc attctctcaa gtcttcggtg
     2521 acgcaaggtc cctagttctt ggccttgtcg accatcttgc gctcggcgat ccacggcatc
     2581 atgtcgcgca gcttcttgcc gacgacctcc atgtcgtggt cgtcgcattt ggcgcgcatg
     2641 gccttgaagc tcggctggtt gatcttgttc tcgagcatcc aatccttcac gaacttgcct
     2701 gactggatat cgctcaagac gtccttcatg gcggctttgc tctgctcgtt caccacgcgg
     2761 gggcccgaga cgtactcgcc gtactcggcg gtgttggaca cggagtagtt catgttggcg
     2821 atgccacctt catagatgag gtcgacgatc agcttcacct cgtggacaca ctcgaaatag
     2881 gccatctcag gggcatagcc tgcctcgacc aacgtctcga agccgttgcg catcagctcg
     2941 acaaggccgc cgcagagcac ggcctgctcg ccgaacaggt cggtctcgca ttcctcgcgg
     3001 aacgtggtct cgatgatgcc ggcgcggccg ccgccgatcg ccgaagcata agagagcgac
     3061 aggtcgtggg cgttgccggt cgcgtcttgc gcgatggcca cgaggcacgg cacgccggcg
     3121 ccgcgcttat attcggcgcg gaccgtgtgg ccggggcctt tcggcgccac catgcacacg
     3181 tccatgtcgg cgcgcggctc gatgagattg aagtggatgt tgaggccgtg ggcgaagagc
     3241 agcgccgcgc cctgcttcat gttgggcttg aggtcgttct cccagatacc ggcctggagc
     3301 tcgtccgggg tgagcatcat caccacgtcg gcccatttgg cggcgtcggc cacggacatg
     3361 accttgagcc cctcgccctc agccttggcg gcggagctgg agccctcacg caagccaaca
     3421 gccacctctt tgacgccgga ctccttcagg ttcagcgcat gcgcgtgtcc ctgactgcca
     3481 tagccaataa tggcgacctt cttacccttg atcagattga gatcggcatc ccgatcgtaa
     3541 tagacacgca tacctcttcc cttcgtcgtc taaatccaat gtgacggctc ggcccgggtt
     3601 gtgccgccgg accggcgaaa aaacaagtgg cgttacatcc gctccgcccc gcgggcaatt
     3661 gccgcaaggc ctgtgcggca gacctcgacc aggccgagcg gctgcatcag ggcgatgaat
     3721 tcctcgatgc gcgactgctc gccggtgatc tcaaagacga aatgctcggt ggacgagtcg
     3781 atgatctcgg cgtgaaacgc ctcggccaga ccaagcgcct cgacccgctt ctcgccggtg
     3841 cctgccacct tgagtaacgc gagctcgcgc tcgatgggct ggccgagcac ggtcagatcg
     3901 gtgaccgagt ggatcggcac gagccggtcg agctgatgct tgatctgctc gatgaccatg
     3961 ggcgtgccgg tggtcacgat ggtgatgcgc gacagatgcg cctcgtgctc ggtctccgac
     4021 acggtcagcg actcgatgtt gtagccgcgg ccagagaaca gaccgatgac gcgcgcgaga
     4081 acgccgggtt cgttgtcgac cagcacgctg atcgtatgcg actccgggtt ctgctcttgc
     4141 ttgtcgagcg agtatgcgga cccaggctgc gtcattacac catcaccttg ccttcgtcgg
     4201 tgatggcgtt ctcgatgtcc tcttcggtga cgtcgtcgcc catcagcatg ttgttgtgcg
     4261 cctccccgct cgggatcatg gggaagcaat tggcatgctt atggacccgg caatcgaaaa
     4321 tgaccgggcg cggcgtctcg atcatctcgc ggatggcgtc gtccagctcc gacggcttct
     4381 ccgcgcgaat gccgacggct tgatacgcct ccgccaactt gacgaagtcg ggcaacgcct
     4441 cggtgtagga atgcgagtag cggccgccat ggagcagctc ctgccactga cggaccatgc
     4501 ccatgtactc gttgttcaag ataaagatct tgatgggcag gtcgtactgc accgccgtcg
     4561 acatctcctg catggtcatg agcaccgacg cttcgcccgc gatatcgatg acgagcgagt
     4621 ccgggtgcgc catctgcacg ccaaccgcgg ccggaatgcc atagcccatg gttccgagcc
     4681 cgccggaggt catccagcgg ttcggctcgt cgaagtggaa gtgctgagcc gcccacatct
     4741 gatgctggcc gacttccgtg gtgacatata cgtccttgcc gcgcgtcagc tcgaacagcc
     4801 gctgaacggc gtattgcggc atgatgatat cgtcgcggtt cttgtagtgc aggcacttac
     4861 gcgcgcgcca tgtgtcgatc tggtcccacc aagtcttcag tgccggctta tcgactttcg
     4921 gcttcttctt tttccacgcc gcgatcatct ggttcagcac gtggtcgcaa tcgccaacga
     4981 tggggatgtc gacctggacg ttcttgttga tcgaggacgg gtcgatgtcg acgtggatct
     5041 tcttggagcc cggcgagaag gcgtcgacac gccccgtgat acggtcgtcg aagcgcgcgc
     5101 cgatgcagat catcacgtcg caatcgtgca tcgcgttgtt cgcttcataa gtgccgtgca
     5161 taccgagcat gccgagccat tgcttgtccg atgccggaaa ggcgccgagc cccatcaggg
     5221 tcgaggtgat cggatagccg gtcatgtgca ccagctcacg cagccgctcg ctggcggcgg
     5281 gccctgcgtt gatgacgcca ccgccggtat agaagatcgg acgcgtggcg gttgccatcg
     5341 cctcaacagc agcttcgacc gcggcggaat cgccttcctt gggcgggcgg tagctcttgt
     5401 gctgcgagtt cgacagcccg gcgtagaggc cggatgcaaa ctggatgtcc ttggggatat
     5461 cgaccacgac aggaccgggc cggcccgaac gcgccacata gaacgcctcg tgcaagacgg
     5521 tcgacaactg gtcaacgtct ttcaccagat agttgtgctt ggtacagggg cgcgtgatgc
     5581 cgaccgtgtc gcattcctgg aacgcatcgt taccgatcag gtgggtcggc acctgtccgg
     5641 tgatgcagac aacggggatc gaatccatca gcgcgtcggt caggcctgtg acggcgttcg
     5701 tcgcgcccgg gcccgatgtc acaagcacga cgccgacctt gccggtggag cgggcatagc
     5761 cttcggcggc gtgaaccgca ccctgctcgt ggcggacgag cacgtgcttc agccggtctt
     5821 gctggaacag ctcgtcatag atcggcagaa cggcgccgcc gggatagccg aagatgtgct
     5881 cgacgccctg gtcggcgaga gcctccagca caatttgggc gcctgtcatc gtgcgtgaca
     5941 ttgtctctac tcattggcaa caccgggccg tcgcggctgg acgcagccgc tgccctggtg
     6001 gaaacgctaa agaatggcgg aaaacgccgg tgtaagccgc gcaccctaag cggtgcatga
     6061 caacgggtca acgagaattg cgaatgaatg acaagcttaa cgctctattg ctttgtgaaa
     6121 attacccgc
//
LOCUS       DBNHVU010000015         3051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_8029, whole genome shotgun sequence.
ACCESSION   DBNHVU010000015 DBNHVU010000000
VERSION     DBNHVU010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_8029"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="AC16Y7_00525"
     CDS             complement(<1..666)
                     /locus_tag="AC16Y7_00525"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase"
                     /protein_id="HIR5707895.1"
                     /translation="MATVQTGSPEWKARAQAVLPPTLLPDLAYTPEVAAETAHLYERV
                     KRVIPPIEWPGFAPYIKAINDLKKARNAVVLAHNYMTPEIFNCVADVSGDSLQLAREA
                     VEADADVIVQCGVHFMAETSKLLNPDKTVLIPDMRAGCSLADSITGADVRALREAYPG
                     VPVVTYVNTSADVKAESDICCTSSNAVRVVESLGVKRVIMIPDEFLAKWVQTQTDVEI
                     ITWK"
     gene            929..2515
                     /locus_tag="AC16Y7_00530"
     CDS             929..2515
                     /locus_tag="AC16Y7_00530"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase scaffold domain-containing
                     protein"
                     /protein_id="HIR5707896.1"
                     /translation="MRRRSVVTILGAATLLGLIALLAVQLATGEQREETAAADIVVAI
                     PDNPASLAATEVQDAEVVAVLPGAEPPQEDAGEASDAPQDLRITDTIRQLLDDPLLHK
                     GAHADDIAAVKAFYTSHSGPALWLTTTGISSNGQAVLREMGRGGEWGFDPSIFQVPPA
                     DFKPASASDQAATEIAISLASLKYARAARGGLAEPSKVFDQTPSVRDPAAVMTAIAAS
                     QSPDAYLTDLHPKHEQFAALRQALAKAQSKENITRIEASMERWRWMPEELGASYVWLN
                     VPEFMLRVVKDGKTVASEKIAVGNTRNPTPVLSADLKEIVLNPERKVPLSVIRRDVLP
                     KLRESGGLFGGGASAIIEQYKLTVKNRGRVIDPDNIDWNKVNLSKLTFVQAPGRTNVL
                     GKVQFLYPNDRGVFMHDTIRRGQLTRAVRAEGSKEPRVANPAKLAGVLLAEDKGWSQA
                     KVNQLIKSGKTRRVKIDKPIPVHTTYFTAVVDDEGEVKTFDDVYKLDKPSKPDDEAAA
                     SPPVAGSPPARKPVRAATAP"
     gene            2533..2724
                     /locus_tag="AC16Y7_00535"
     CDS             2533..2724
                     /locus_tag="AC16Y7_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707897.1"
                     /translation="MQSQFTVGINCEGKGDRVVVNAEDALIAALKVKAERPEATITYV
                     RRLNRRGDQRHPPHRLAEK"
     gene            complement(2738..>3051)
                     /locus_tag="AC16Y7_00540"
     CDS             complement(2738..>3051)
                     /locus_tag="AC16Y7_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707898.1"
                     /translation="ALSGTIAELASATVLLLLSVFTVVNGALFVLKGRKGEEPGRFEI
                     PRIIPALGASVCLALIVVRVSTGDWRAPAIAGGLLLGCFVLYMAMRAIGSARHALEED
                     S"
BASE COUNT          647 a          915 c          956 g          533 t
ORIGIN      
        1 cttccaggtg atgatctcga cgtccgtctg agtctgcacc catttggcga gaaactcgtc
       61 ggggatcatg atcacgcgct tgacgccgag cgactccacg acgcgaaccg cgttcgaaga
      121 cgtacagcaa atgtcggact ctgccttaac gtcggcggag gtgttgacgt aggtcaccac
      181 cggcacgccg ggataagctt cgcgcagcgc acgcacatcg gcgccggtga tggaatccgc
      241 gagcgaacag cccgcgcgca tatccggaat cagaaccgtc ttgtcggggt tcagcagctt
      301 cgaggtctcg gccatgaagt gaacaccgca ttgcacgatg acgtccgcgt ctgcttcgac
      361 cgcctcgcgg gcgagctgga gggagtcgcc ggacacgtcg gcaacgcagt tgaagatctc
      421 aggcgtcatg tagttgtgcg ccagcacgac cgcattacgg gccttcttaa gatcgttgat
      481 cgctttgatg tagggagcaa aacccggcca ctcgatcggc gggatcacgc gtttcacgcg
      541 ctcataaaga tgtgcggtct ctgccgccac ttcgggcgta taggcaagat ctggcaatag
      601 ggttggcggc agtaccgcct gagcccgtgc tttccattca gggctaccag tttgaactgt
      661 tgccatgaaa tcctgcctcc catcgctatt aatactcaat atgagtatat atggggtcta
      721 aaaaggtccg gcacaaggcc ggcacgtccc aattctcaaa aagagtatag gcgattgacg
      781 caccgcatca agagtgggcc atccgctgtg gggacaagag atctggcaga aaccggcgaa
      841 attcaagact ttgcccggcc aagaggcaat tcttgctgga aggaggtttc tccggggact
      901 aacctaggtc catagtgggg ttcttgggat gcgtaggcgg tctgtagtca cgatcctcgg
      961 tgcggcgacc cttttggggc tcatcgcatt gttggcggtt cagctggcca ctggcgagca
     1021 acgcgaggag accgcggccg ccgacatcgt cgtcgcgatt cctgacaatc cagccagcct
     1081 tgcagcgacc gaggtgcagg acgccgaagt cgttgccgta ctgccgggtg cggagcctcc
     1141 gcaagaggac gcgggcgagg cttcggacgc cccgcaagac ctgcgcatca ccgatacgat
     1201 ccgtcagctg ctcgacgatc cactgctgca caaaggcgct catgcggacg atatcgcggc
     1261 ggtcaaggcg ttctacacct cccatagcgg gcctgcgctg tggctgacca cgacgggtat
     1321 ttcgtcaaac gggcaagctg tgctgcgcga gatgggacgg ggtggtgagt ggggcttcga
     1381 cccaagcatc ttccaggtgc cgcctgcgga ctttaagccc gcctcggcat cggaccaggc
     1441 ggccaccgag atcgcgatca gtctcgcatc cctgaaatat gcacgcgcgg cacgcggcgg
     1501 tctcgccgag ccgagcaaag tcttcgatca gacgccttcg gttcgcgatc cggcagccgt
     1561 catgacagcg atcgccgcat cgcaatcgcc ggacgcctat ctcacggatc tgcaccccaa
     1621 gcatgagcag ttcgcagcct tgcgccaagc gctcgccaaa gcacagtcga aagaaaacat
     1681 cacgcgcatc gaagccagca tggagcgctg gcgctggatg ccggaagagc tgggcgcatc
     1741 ctatgtgtgg ctcaacgtcc cggagttcat gttgcgcgtg gtcaaggacg gtaagaccgt
     1801 ggcgtcggag aagatcgcgg tcggcaatac cagaaatcca acgccggtct tgtccgctga
     1861 cctcaaggaa atcgtcctca atccggagcg caaggtcccg ctgtcggtca ttcgccggga
     1921 cgtcttgccc aaattgcgcg aaagcggcgg gctgttcggc ggcggggctt ctgcgatcat
     1981 cgagcagtac aagttgaccg tgaagaaccg gggcagggtg atcgacccgg acaatatcga
     2041 ttggaacaag gtcaacctgt ccaagctaac cttcgttcag gcgccgggcc gcaccaatgt
     2101 cttgggcaag gtccagttcc tctatccgaa cgatcgtggc gtctttatgc atgacaccat
     2161 ccggcgcgga cagttgacgc gcgccgtccg ggctgaaggg tctaaggagc cgcgcgtcgc
     2221 gaaccctgcg aagctcgcag gcgttctgct cgccgaggac aagggctgga gccaggcgaa
     2281 ggtcaatcaa ctgatcaaga gcggcaagac gagaagggtt aagattgaca agcccatccc
     2341 ggtccacacg acctacttta cggctgtagt cgacgatgag ggcgaggtta agacctttga
     2401 cgatgtttac aagctcgaca aaccgagcaa gccggacgac gaagcagccg cgtcgccgcc
     2461 tgtcgcaggt tcgcctcccg cgcgaaagcc tgtaagggcg gccaccgcgc cttagcaccg
     2521 agggcagggg ctatgcagtc gcagttcacc gtcggaatca actgcgaggg gaagggcgac
     2581 cgcgtcgtcg tcaatgcgga ggacgcgttg atcgcggcgc tcaaggtcaa ggccgaacgg
     2641 cctgaagcaa cgatcaccta tgtgcgccgt ctcaaccgcc gcggcgatca gcgtcacccg
     2701 ccgcaccgcc ttgccgagaa gtaaggcccc acgccggcta gctatcttct tctaaagcat
     2761 ggcgcgcgga accgatcgcg cgcatggcca tatagaggac gaagcaaccc agcagtagcc
     2821 cgccggcgat ggcgggcgcg cgccaatcgc cggttgagac gcgaacgacg ataagggcga
     2881 ggcacaccga ggcgccaagc gccggaatga tgcgcggaat ctcgaatcgg ccgggctctt
     2941 ccccttttct acctttcaag acgaagaggg cgccgttcac gacggtgaag acggagagca
     3001 gaagcaacac agtcgcggag gcgagctcgg cgatcgtgcc ggagagcgcc a
//
LOCUS       DBNHVU010000016        18610 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_601423, whole genome shotgun sequence.
ACCESSION   DBNHVU010000016 DBNHVU010000000
VERSION     DBNHVU010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 18610)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18610)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18610
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_601423"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="AC16Y7_00545"
     CDS             complement(<1..476)
                     /locus_tag="AC16Y7_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013252.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HIR5707899.1"
                     /translation="MVLARGSKKNSSGTAHVKSKRARKVKAHAFVAPQLATLAADTPT
                     SEEWLHEIKYDGYRIEARKAGDDITLFSRSGLDWTQRFPGIARAMQTLPCKSALIDGE
                     AAFVLPSGLTDFKSIQEHIDTPHGAIRYFAFDLLFLDGKDLRREPLTTRKAKLRDL"
     gene            complement(425..1072)
                     /locus_tag="AC16Y7_00550"
     CDS             complement(425..1072)
                     /locus_tag="AC16Y7_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5707900.1"
                     /translation="MTRARSDDPLATYKAKRDFGKTPEPGPKRVRKTGNSFVIQKHAA
                     RRTHFDFRLEHDGVLKSWAVTKGPSLDPSQKRLAVQTEDHPLEYGGFEGVIPKGEYGG
                     GPVMIWDEGTWEPLDDPDEGLAKGDLKFRLHGKRLHGDYVLVRMKKDRRGGKRENWLL
                     IKKRDAYAGDGAEPTEDYDTSVRSGRTMDEIATGADSAVWSSRAAQKKTPAAQHT"
     gene            complement(1076..1945)
                     /gene="ku"
                     /locus_tag="AC16Y7_00555"
     CDS             complement(1076..1945)
                     /gene="ku"
                     /locus_tag="AC16Y7_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end joining protein Ku"
                     /protein_id="HIR5707901.1"
                     /translation="MAPRAFWTGHLRLSLVNIPVRLYPATTSERAIAFHQIHEPTGKR
                     VRYQKIVPEVGAIENEEIVKGYEYEKGKYVLIDPTEIDELKLESKQSIEIVRFVDEDS
                     IDTRYFQTPYYLMPDGDMADEGYVLLRRALTETKKVGVGQFILRGHGNIVAIKPFEEG
                     LLLEVLRHADAIRPAQKLFDEIPDVKIDKEALELAKELVTRKSGEFQPEQFKDEYAAA
                     VWELIQAKLEDRAPDIVTEEPGGAKVINIMDALKKSVGKRKGSGAKPRAKKTAPSRSS
                     AKKTARGAKRKSA"
     gene            complement(2049..3176)
                     /locus_tag="AC16Y7_00560"
     CDS             complement(2049..3176)
                     /locus_tag="AC16Y7_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707902.1"
                     /translation="MYLLLFGTLSLVGLAPALAEPSLSEAIETADASYLAGPESRTID
                     DTLKIVAGGGEEGLPPVDEELVLGPLRVTLTYVEEGPGGGAPADNGEIPLEDIPADAM
                     LPGEFDLGETVARLAVVRVHLEDPNAADRVAASAESEIGGSDQTDGEATGPRVVAELP
                     GPSTGVSDPPVSIQIAELDPGNAHPEVVVSFFTGGAHCCSVSQVVTGNADGSEWTTVD
                     VGEFDGGPLLAVDLDGDGTYEFETRDNAFLYAFACYACSQAPLQVLALRSGKIKNVGA
                     EPRFKPAHAAWLKDMIVGVPEEDVNGFLAGYVGQKIRLGEGKQAWDLMLRHHDRSADW
                     GLEVCDTERDEGGECPGQTTKVTFPEALKRMLNKNGYMIGG"
     gene            3404..4276
                     /locus_tag="AC16Y7_00565"
     CDS             3404..4276
                     /locus_tag="AC16Y7_00565"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5707903.1"
                     /translation="MNASGETPQPLAPASIITGATQGIGRALVDEFAKGGHTLLLVAR
                     TETALAEIARETADKYGVAVHYVPCDLSTSEGCDRVAEALDANGLYCESLVNNAAIMT
                     AGFFHDLDKDILVQKVDLNVRAAVDLTRRFLPDMIARGQGGVLNVASLEGFMPVPYQA
                     TYAATKGFLIAWSRSLAWEVFGTGVRICVATPGPIDTTIHEKGGAQHSRYMVYLPRHS
                     AEQAAEAAYRRFMRGNWVIVEGWVNRIIASGVRFIPGVMLIPAVGWFFRVRDAAGNLK
                     EPKPQKHPDQASGD"
     gene            complement(4306..4381)
                     /gene="ssrA"
                     /locus_tag="AC16Y7_00570"
     tmRNA           complement(4306..4381)
                     /gene="ssrA"
                     /locus_tag="AC16Y7_00570"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            4541..4834
                     /locus_tag="AC16Y7_00575"
     CDS             4541..4834
                     /locus_tag="AC16Y7_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008539240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR5707904.1"
                     /translation="MVPLIGFFVLVIDLYIWVVIASAILSWLIAFNVVNTNNRIVLTI
                     ADALYRITEPALRPIRSILPSLGGIDISPVILILLLLFIRDVVLLGWILPAVV"
     gene            4894..5169
                     /locus_tag="AC16Y7_00580"
     CDS             4894..5169
                     /locus_tag="AC16Y7_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014633.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HIR5707905.1"
                     /translation="MPVRLTPKSAHDAVEGLQDFAGETVLKARVAAVPEAGRANAAVE
                     TLIAKWLGVPASKVTVSHGANSRLKQVAIEGDAVMLSALIAARIVAL"
     gene            complement(5170..5886)
                     /gene="pyrF"
                     /locus_tag="AC16Y7_00585"
     CDS             complement(5170..5886)
                     /gene="pyrF"
                     /locus_tag="AC16Y7_00585"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR5707906.1"
                     /translation="MTTDTVSPKDRIIVALDVPHVDEARALIKKLGDSVGVFKIGLEL
                     IYAGGLALAEELAKDGHRVFLDAKLLDIEATVERATAAIAKTGAHFLTVHATDRKTLN
                     AAVRGRGDSALKLLGVTVLTNLDRSDLSEQGIDMPPLALVQERARLSQDAGFDGIVAS
                     GKEAAALHERLRNFLIVTPGIRQAGTDVQDQTRVVTPTIAVQSGADYLVIGRPITRAD
                     DPRAAAQEIAEEIAGAIEAG"
     gene            complement(5883..6446)
                     /locus_tag="AC16Y7_00590"
     CDS             complement(5883..6446)
                     /locus_tag="AC16Y7_00590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase [NAD(P)H] activity
                     [Evidence IEA];
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HIR5707907.1"
                     /translation="MRLLFFAGSARKGSHNKKLARLGQHIAEANGIEAVFVDLADFYM
                     PIYHGDLEEAEGVPERARAFKALLDEYDGVMIVTPEYNASVPALLKNTLDWVSRVKAE
                     EDVFKTRVFAISGASPGYYGAMRSLLQLREILSVGLGATVIPQQMALPRAMQAFEEDG
                     SLKDDNQQKMFADVVEALAVATRKFAA"
     gene            complement(6459..7544)
                     /locus_tag="AC16Y7_00595"
     CDS             complement(6459..7544)
                     /locus_tag="AC16Y7_00595"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR5707908.1"
                     /translation="MAREPAKPSTRRPASSPAPMSAGQAAKITVAVLAVIALAFALWY
                     AQTAILLLFAGILLAIVLYGASHDLSELTGLPRLLMLAIVSIAVALGLALAAWTAGPT
                     LANQIALLAESVAAGVTTLTTELSSFVKHTAFLKSIDLAQLLGNFMSQWGIATGATSV
                     AISLFGLFSAGLIVVFFGVYLAADPHTYVNLLARLAPPEKRGRVVELLYETGELLRRW
                     LIGQSITMSVIGGFTFVGLLILGVPIAFVLAMFAGLACFLPYLGPIIGAIPILLVAGG
                     ESFHMALWALGLYVCVQFLESYLLTPLIQARAVSMPPAAVILNQLVFGAVFGLVGLAL
                     ATPLAAAATVPLREVFGIGENGNTKKT"
     gene            complement(7650..8807)
                     /gene="dnaJ"
                     /locus_tag="AC16Y7_00600"
     CDS             complement(7650..8807)
                     /gene="dnaJ"
                     /locus_tag="AC16Y7_00600"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR5707909.1"
                     /translation="MVKRCYYDILGVERSAGDPEIKSAFRSLAKECHPDRCNGDPSAE
                     TRFKEVNEAYEALKDPQRRAAYDRFGHAAFENGGGGFGGPGHGFGSDFSASMSAMFDD
                     LFGEFMGAGAGARRPRQRSGRERGADLRYNMEIELAEAYEGKTAQICVPTSVTCDSCE
                     GSGAKAGTVPAACRTCNGMGKVRASQGFFTIERTCPACQGRGETIETPCPSCNGAGRV
                     MRERTLSVSIPVGVEDGTRIRLAGEGEAGLRGGPPGDLYIFLSIKPHEFFQRDGADLF
                     CRVPIAMTTAALSGQVEVPTIDGGRSRVKIPEGTESGKQFRLKSKGMPVLRSKLQGDL
                     YIQVEVETPKNLTRKQRDLLKDFEDASNPDTSPASAGFFARVKEFFEGISD"
     gene            complement(8916..10841)
                     /gene="dnaK"
                     /locus_tag="AC16Y7_00605"
     CDS             complement(8916..10841)
                     /gene="dnaK"
                     /locus_tag="AC16Y7_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041594641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR5707910.1"
                     /translation="MAKVIGIDLGTTNSCVAVMDGANPKVIENAEGMRTTPSMVAFTD
                     DDEVLVGLPAKRQAVTNPENTFFAIKRLIGRRWDDPTVAKDQKLVPYKLVEADNGDAW
                     VESHGKKYSPSQISAYILQKMKETAESHLGQTVEQAVITVPAYFNDAQRQATKDAGKI
                     AGLEVLRIINEPTAAALAYGLDKKEGQTIAVYDLGGGTFDVSILEIGDGVFEVKSTNG
                     DTFLGGEDFDMRIVDYLADEFKKEQGIDLRSDKLALQRLKEAAEKAKIELSSTQQTEV
                     NLPFITADQSGPKHLTMKLTRAKLESLVEDLIKRTIAPCEAALKDAGLKPGEITEVIL
                     VGGMTRMPKVADTVKTFFGKEPHKGVNPDEVVAIGAAIQAGVLQGDVKDVLLLDVTPL
                     SLGIETLGGVFTRLIDRNTTIPTKKGQVFSTAEDNQNAVTIRVFQGEREMAADNKMLG
                     QFDLVGIPPAPRGVPQIEVTFDIDANGIVNVSATDKATGKEQSIRIQASGGLADADID
                     RMVKEAESHAEEDKKRRELVEARNQAEALVHSTDKMLADFGDKVSAEDKAAIEAAVAE
                     LKTAAEGEDPEAIAQKVDAVAQASMKLGEAMYQTSQGAAGGEEEGGPSAEAGSPDEDV
                     VDVDFEEVKDDDDKKSA"
     gene            11139..11735
                     /locus_tag="AC16Y7_00610"
     CDS             11139..11735
                     /locus_tag="AC16Y7_00610"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB
                     family protein"
                     /protein_id="HIR5707911.1"
                     /translation="MRHSPQALNEAGQRALLAEIRAVIAKAPLFVPTMPRSGNPMSVR
                     MTNAGPLGWVTDKTHGYRYQATHPVTGELWPPMPQTVLDLWEALAGHPHPPEACLINY
                     YGGAAKMGLHQDKDEEDFDAPVLSVSLGDTGIFRVGGKTRKSPTEKYELKSGDVFLLG
                     GEDRLAYHGIDRILAGTSDLLEEGGRFNLTLRRVTKPG"
     gene            complement(11744..12379)
                     /gene="grpE"
                     /locus_tag="AC16Y7_00615"
     CDS             complement(11744..12379)
                     /gene="grpE"
                     /locus_tag="AC16Y7_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006940247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR5707912.1"
                     /translation="MADDNSDPRETGEDQAQPDAPETAVDPDASAELAGDLEGLLTEN
                     ADMRDKLLRTMADMENLRRRTEREKEDTARYAISNFARDVLAIGDNLRRTLEHVPEDA
                     VAQDPTLKSIVEGVELTERDMLKMLEKHGVAKLEPLGERFDPNQQQAMYEVPTTEVPE
                     GTVVQVMQAGYTIGDRVLRPALVAVSKGGPKPAPEAPQRGPADDDIPGGGD"
     gene            complement(12462..13559)
                     /gene="hrcA"
                     /locus_tag="AC16Y7_00620"
     CDS             complement(12462..13559)
                     /gene="hrcA"
                     /locus_tag="AC16Y7_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR5707913.1"
                     /translation="MGHTDVKGAKAAAEAAVNLRQLNERSREVFKRIVETYLETGEPV
                     GSRNLSRALPMTLSPASVRNVMSDLESIGLVYAPHTSAGRLPTQTGLRLFVDGLLEIG
                     DITEAERKEIETRMAGGGRRRGVEDFLAEAGEMLSGLSRCAGVVLAEQQQAKTLRQVE
                     FVDLGKGQALAVLVAEDGDVENRIVTLPEGLPLSALSAATNYTNARIAGLTLAEARNR
                     IEGEIEAHRAQLDELTARVVTEGLATWSGDADDRQSLIVCGRAHLLEDVRAMEDLERV
                     RHLFEDLDAKKDLIQLLGLAESADGVRIFIGSENKLFSLSGSALIVSPFEDSEQRIVG
                     VLGVIGPTRLNYARIIPMVDYTARLLGRLVP"
     gene            complement(13645..14529)
                     /gene="olsB"
                     /locus_tag="AC16Y7_00625"
     CDS             complement(13645..14529)
                     /gene="olsB"
                     /locus_tag="AC16Y7_00625"
                     /EC_number="2.3.2.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043810 - ornithine-acyl [acyl carrier
                     protein] N-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="l-ornithine N(alpha)-acyltransferase"
                     /protein_id="HIR5707914.1"
                     /translation="MGWRRAPAGFRGRFQQPRIYGRMGPLEVRLAQKKSELRRAQRLR
                     YKVFYEEMSATPGALAMLSRRDEDAFDPIFDHLLVMDQGDPNKKGWRRPSVVGTYRML
                     RQDIAELHDGFYTQGEYDIAPLIRAKADYSFIELGRSCVLKPYRNRRTLELLWQGVWS
                     YVREHGADVMIGCASFPGTDPSAHALALSFLHHNSLAPEDWRVRAHDHLRVDMNMMPK
                     DAVNTRAALKALPPLIKGYLRVGAFVGDGAVIDHQFGTTDIFVIMPVKHINSRYFAHF
                     GAPDELNSQIAPKTGALN"
     gene            complement(14823..17135)
                     /locus_tag="AC16Y7_00630"
     CDS             complement(14823..17135)
                     /locus_tag="AC16Y7_00630"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing hybrid sensor histidine
                     kinase/response regulator"
                     /protein_id="HIR5707915.1"
                     /translation="MQRKTWQSFGADCAGGIGARVALSGLAVALVALSAVLLGAIKTF
                     ALASLGAGVVLLILALRQNGKTGNARVESLASRLDSNLEALKDLQWEVREREARYRDL
                     LDHQGDVILRRDVDGHLSFVNDAFCQTFGTSRDAALGQVFELPVLSCDSTAAAMKGGG
                     RHSRVVEISTVSGARWFVWEDFAIADDDGNMSEVQSVGRDVTEQRAAELELAQARDDA
                     MIASDAKSRFLASVSHEIRTPMSGILGMTGLLLDTDLSPEQHTYARAVSTSAKTLLSL
                     IDEVLDFSKIEAGKIELHNAPFDIADAAQNVVELLAPRARDKGLEIGGFVAPDLPKTV
                     IGDELRVRQILLNLVGNAIKFTDHGGVALTLNMAPGSQQDDRAVMRFAVRDTGPGVPP
                     EALTRIFSEFEQADQGRARQHGGTGLGLAISKRLVDEMGGNIDVTSVPGAGATFTADL
                     PFETPTPPERVGESWPGPQITEHVLIVLNGSMEAALVGELLLGMGASVVRARPEDAAR
                     TVSDAARKGMPFTAVLTDKPSIEAGADRLPALVAAKGCEHPRAVVVIDPSERADMPKF
                     REHGFGGYLVRPVRPLSVLTQLFGDTAQDHAPEDQASHDQAPCAATPEALPAPTLDEP
                     DCLAELSVLLAEDNDINALLARTVLEKGGARVVRARNGADAIAEARAALTAARGFDLV
                     LTDIHMPGMDGVEAARRIRALYPDDAGPGAGRPPIVALTANAFAEDRTAYLAAGLDDY
                     LAKPFETRDLAALLSHWHIRETEETGAGAA"
     gene            complement(17239..17671)
                     /locus_tag="AC16Y7_00635"
                     /pseudo
     CDS             complement(17239..17671)
                     /locus_tag="AC16Y7_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950100.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase subunit ChlI family protein"
     gene            17759..18220
                     /locus_tag="AC16Y7_00640"
     CDS             17759..18220
                     /locus_tag="AC16Y7_00640"
                     /inference="COORDINATES: protein motif:HMM:NF024636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HIR5707916.1"
                     /translation="MPRDPRTLALLAHLAAYLVAVAISAAVNLWLSPDRLWFVWIAAG
                     WGIGVAAHALAFWLAHTRRRERVFLDPHARGFAVHAFAYVAVVVLLFIVNVTVTPKVW
                     WFYWVALGWGAGVLAHGWCVFFRKCAPSGRRPPSPRLRPRSLRRYPRRRTP"
     gene            complement(18144..>18610)
                     /locus_tag="AC16Y7_00645"
     CDS             complement(18144..>18610)
                     /locus_tag="AC16Y7_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR5707917.1"
                     /translation="YGNGGAAIFRIAEGDTNLNSLIELLGLTFREPIMVQRYLPEVRA
                     GDKRIILVDGEVAGAINRVPAADETRSNLHVGGTAKATTLSPREDEICARLGPELKKR
                     GLIFAGIDVIGDYLTEINVTSPTGIRQVKEFDGVDIAAMIWDVIEEKVAAGR"
BASE COUNT         3209 a         5940 c         5887 g         3574 t
ORIGIN      
        1 aacaggtcac gcagctttgc cttacgtgtc gtgagcggtt ctctccgcaa atccttgccg
       61 tcgagaaaca gaaggtcgaa cgcgaagtag cggatcgcgc catgcggcgt atcgatgtgc
      121 tcttgaatcg acttgaagtc ggtcaatccc gacggcagca cgaacgcggc ctcgccgtcg
      181 atcagagcgc ttttgcaggg aagcgtctgc atcgcccgcg cgattccagg aaagcgctgc
      241 gtccagtcga gcccgctacg gctgaacagg gtgatgtcgt cgccggcttt gcgcgcttcg
      301 atgcgatagc cgtcatactt gatctcgtgc agccattcct cgctcgtcgg tgtgtcggcg
      361 gcgagcgtgg caagctgcgg cgcgacgaag gcgtgcgctt tgacctttcg ggcacgcttg
      421 gactttacgt gtgctgtgcc gctggagttt ttttttgagc cgcgcgcgag gaccacacgg
      481 cagagtccgc gcctgtcgcg atttcgtcca tggtacggcc tgatctgacg ctggtgtcgt
      541 agtcctccgt gggctccgcg ccatcgccgg cgtaagcgtc gcgcttcttg atcagtagcc
      601 agttctcgcg cttgccgccc ctgcgatcct ttttcatgcg gaccaacaca tagtcgccat
      661 ggaggcgctt gccgtgcagc cggaacttca gatcgccttt ggccagccct tcgtcgggat
      721 cgtcgagggg ttcccacgtc ccttcgtccc agatcatcac cgggccgccg ccatattcgc
      781 ccttcgggat cacgccctcg aagccgccat attcgagcgg gtgatcttcg gtctgaacgg
      841 caagccgctt ctgggacgga tcgaggctcg ggcctttggt gacggcccag ctcttcagca
      901 cgccgtcatg ctccaggcgg aaatcgaaat gggtccgccg cgccgcgtgc ttctggatga
      961 cgaaactgtt gcctgtctta cgcacgcgct tcggcccggg ctcgggcgtt ttgccgaagt
     1021 cccgctttgc cttgtaggtg gcaagcggat cgtcacttcg ggcgcgcgtc atagcttaag
     1081 ccgatttgcg cttcgcgccg cgcgctgtct tcttggcgct cgaccgcgag ggcgcggttt
     1141 ttttggcgcg cggcttcgcg cctgaacctt tgcgcttgcc tacgctcttc ttcagcgcgt
     1201 ccatgatgtt gatgactttc gcgccgcccg gctcttcggt gacgatgtcc ggcgcgcggt
     1261 cctcgagctt agcctggatg agttcccaca cggcggcggc gtattcgtcc ttgaactgct
     1321 cgggctggaa ctcgcctgat tttcgcgtga cgagttcctt ggccagctcg agcgcttcct
     1381 tgtcgatctt cacatctgga atttcatcga agagtttctg agcgggccga atagcgtccg
     1441 catggcgcaa cacctccaag agtaggccct cctcgaaggg cttgatggcg acgatgttgc
     1501 cgtggccgcg caaaatgaat tgtccgacac cgactttctt tgtttcagtt agcgcgcggc
     1561 gtaggagcac atagccttcg tcggccatgt cgccgtccgg catcaaatag tagggcgtct
     1621 ggaaatagcg cgtatcgatg gagtcctcgt cgacgaagcg gacgatttcg atcgactgct
     1681 tggactccag tttgagctcg tcgatctccg tggggtcgat caggacatat tttcccttct
     1741 cgtactcgta gcccttgacg atctcctcgt tctcgatcgc gccgacctcg ggcacgatct
     1801 tttggtagcg cacgcgcttg cccgtcggct cgtgaatctg gtggaaggcg attgcccgct
     1861 cggacgtggt ggccggatag agcctgaccg ggatgttgac gagcgacagc cgcaaatggc
     1921 cggtccagaa agcgcgcggt gccatgcgcc gtactcctta catgaacgcg taaaacctcc
     1981 ccggatccct tggcaggtaa ggccaggttg ggaagggcgc cggccccggc gtttgcttga
     2041 gggcgggctc agccccctat catatagccg tttttgttaa gcatgcgttt gagcgcctca
     2101 ggaaacgtga ctttcgtggt ctggccaggg cattccccgc cctcgtcgcg ttctgtatcg
     2161 cagacttcca gcccccaatc ggcgctccgg tcgtggtgcc ggagcatcaa gtcccaggcc
     2221 tgcttgccct cgccgagccg gattttctgg cccacatagc cagccagaaa gccgttcacg
     2281 tcctcttcgg gtacgcccac gatcatgtcc ttgagccagg ccgcatgggc cggcttgaag
     2341 cggggctccg cgccgacgtt cttgatcttg ccgctgcgaa gcgcaagcac ctgaagcggc
     2401 gcctgcgagc atgcatagca ggcaaacgca taaaggaagg cgttgtcacg cgtctcgaac
     2461 tcgtaagtgc cgtcgccgtc gagatcgact gccaataaag gcccgccgtc gaattcaccg
     2521 acatcgaccg tggtccattc ggagccgtcg gcgttgccgg tcacaacctg actcaccgag
     2581 cagcaatgcg cgccgccggt gaagaacgac accaccactt ctggatgggc attgcctgga
     2641 tcgagctccg cgatctggat gctaaccggc ggatcggaaa cgccggtgct cgggccggga
     2701 agttcggcga cgacacgtgg accggtggcc tcaccgtccg tctggtcgga accgccgatc
     2761 tccgattcag cgcttgccgc gacgcgatct gctgcgttgg ggtcttcgag atggaccctc
     2821 acgacggcca ggcgcgcgac cgtttctcca aggtcgaact ctcccggcag catggcatcc
     2881 gccgggatgt cctccagggg aatctcgccg ttgtcggccg gtgccccgcc acccggtcct
     2941 tcctcgacat aggtcagcgt cacgcgcaag ggaccgagga cgagttcctc gtccacgggc
     3001 ggcaggcctt cctcgccgcc gccggcaacg attttgagcg tgtcgtcaat ggtgcggctc
     3061 tccggacccg ccagatagct ggcatcggcg gtctcgatcg cctcgcttag gctgggttcg
     3121 gcgagggcgg gcgcgaggcc cacaagcgaa agcgtcccga aaaggagaag atacaaggaa
     3181 gcgattggtg acattgcgca gcgttttcca agtctgacaa ataacagcca acgtgaatca
     3241 gtaacattct agcgcggcgc ccagccggcc cccaacccat tggaggcatg agggttaccg
     3301 gaatacacgg cgcccgcgcg cggccacaga tggtagggta tgggcttgcg tgcaaggggt
     3361 gctcggacgt atctgagcgc tcagcaagtg gcattggatg gacatgaacg catctggtga
     3421 aacaccgcag ccgctggcgc ctgcctccat catcaccggc gcgacgcaag gcattggccg
     3481 cgcgctcgtg gatgaattcg ccaagggcgg ccacacattg ctgctcgtcg cccgcaccga
     3541 gacggcgctc gctgagatcg cgcgcgagac ggcggacaaa tacggcgtcg ctgttcacta
     3601 cgtgccgtgc gacctgtcga cgagcgaagg ttgtgaccgc gtggccgagg ctctcgacgc
     3661 aaacgggctc tactgcgagt cgctggtcaa caacgccgcg attatgacgg ccggcttctt
     3721 tcacgatctg gacaaggaca tccttgtaca aaaagtcgat ctcaatgtgc gcgcggcggt
     3781 cgacctcacg cgccggttcc tgccggacat gatcgcgcgg gggcaaggcg gcgtgcttaa
     3841 cgtggcctcg ttggaaggct tcatgcccgt cccctatcaa gccacttatg ccgccaccaa
     3901 ggggttcctg atcgcctgga gccgatcgct cgcttgggag gtcttcggca ccggggttcg
     3961 catttgcgtc gccacccctg gtcccatcga caccacgatc cacgagaagg gcggcgctca
     4021 gcattcgcgc tacatggtct atctgccccg gcacagtgcc gaacaggcgg ccgaagcggc
     4081 ttaccggcgg ttcatgcgcg gcaactgggt gattgtcgaa ggctgggtca accggattat
     4141 cgcgagcggc gtgcgcttca tcccaggtgt gatgctgatc cccgcggtgg gctggttctt
     4201 ccgggtccgc gacgcggcgg gaaacctgaa agaaccgaag ccccagaagc atcccgatca
     4261 ggcttcaggc gactgagctc cgcgcgggta tgacgattgg ggatttggtg gagccgaggg
     4321 gaatcgaacc cctgacctca gcagtgcgat tgctgcgctc tcccaactga gctacggccc
     4381 caggaagtcc gcatttaggg aatggcctcc atacctgtca agcgatggcc cgccgcggca
     4441 gtggaagcgg gacaaacggc atccaggccc tctcaaggct tgccctcgcc tacacctctc
     4501 cgctacatgg accacacggc cgcgccagga ggggtctttt atggtgcctt tgatcggatt
     4561 tttcgtcctt gtgatcgatc tctacatctg ggtggtgatc gcgagcgcga ttctgagctg
     4621 gttgatcgcc ttcaacgtgg tcaacaccaa taaccgtatc gtcctgacca tcgcggacgc
     4681 gctctaccgg atcaccgaac cggccttgcg cccgatcagg agtatcttgc ccagtctcgg
     4741 cggcatcgac atctcgccgg tgatcctgat tctcctgctg ttgttcatcc gcgacgtggt
     4801 gctgctcggc tggatcctgc cggctgtcgt ctagccaggg ccgacggcac ggcctcaagc
     4861 ctcaatatcc gcaagaccgc caaaggcgtc atcctgcccg ttcgcctgac gccaaagtcg
     4921 gcgcacgatg cggtcgaggg ccttcaggac tttgccggcg agacggtgct gaaggcgcgc
     4981 gtggccgcag tgcccgaagc aggccgcgcc aatgccgcag tggagacgct gatcgccaaa
     5041 tggcttggcg tccctgcctc aaaggtcacg gtcagccatg gcgccaattc gcgcctgaag
     5101 caggtcgcca tcgaaggcga tgcggtcatg ctctcagcgt tgatcgcagc gcggatcgtg
     5161 gcgctctgat cagccggcct cgattgcgcc ggcgatctcc tcggcaatct cttgcgccgc
     5221 cgcgcgcggg tcgtcggcgc gcgtaatggg acggccgatg acgagataat ccgcgcccga
     5281 ctggaccgcg atcgtcggtg tcaccacgcg agtctgatcc tgaacgtcgg tgcccgcctg
     5341 gcggatcccc ggcgtcacga tcaaaaagtt gcgcaagcgc tcatggagcg ccgccgcctc
     5401 cttgcccgaa gcgacgatgc cgtcgaagcc cgcatcttgc gaaagccgcg cgcgctcctg
     5461 caccagcgcc agcggtggca tgtcgatgcc ctgttcggat agatccgagc ggtcgagatt
     5521 ggtcagcacc gtcacgccga gaagcttgag cgcagaatcg ccgcggccgc gcacggcggc
     5581 gttgagcgtc ttgcggtcag tcgcatgcac ggtgagaaaa tgtgcaccgg tcttggcaat
     5641 ggcggccgtg gcacgctcga ccgttgcctc gatgtcgagg agctttgcgt cgagaaacac
     5701 acgatgtccg tcttttgcaa gttcctcggc aagcgccaag ccgcccgcgt agatcagttc
     5761 gagcccgatc ttgaacacgc cgacgctgtc gccaagcttc ttgatgagcg cgcgcgcctc
     5821 gtccacgtgg gggacgtcca gcgccacgat gatgcggtcc tttggagata cggtgtccgt
     5881 tgtcatgcgg caaacttcct cgtagcgact gccagcgctt cgaccacgtc ggcaaacatc
     5941 ttttgctgat tgtcgtcctt caagctgccg tcttcttcga acgcctgcat ggctcgcggc
     6001 agcgccatct gttgcgggat cacggtggcg ccaaggccca cgctgagaat ctcgcgaagt
     6061 tgcaacaggc ttcgcatggc accgtaatag ccgggcgacg cacctgagat cgcgaagacg
     6121 cgcgtcttga acacgtcttc ctccgctttg acccgcgaca cccaatcgag cgtgttcttc
     6181 aaaagcgccg gaacggaggc gttgtactcc ggggtcacga tcatcacgcc atcatactcg
     6241 tcgagcagcg ccttgaaggc acgggcgcgc tcgggcacac cctctgcttc ctccaggtcg
     6301 ccgtgataaa tcggcatgta aaagtcggcg agatcgacaa agacggcctc gattccgttc
     6361 gcttcggcga tgtgttgccc cagtcgggca agtttcttgt tgtgtgagcc cttccgggcg
     6421 ctgccggcaa agaacaatag acgcatacaa gtctcccgtt atgtcttctt ggtgttgccg
     6481 ttctcgccaa taccgaacac ctcacgtaaa ggcacggtag cggcggcggc gagcggcgtg
     6541 gccagcgcga gcccgacaag gccgaacacg gcgccaaaca cgagctggtt gaggatcacg
     6601 gccgccggcg gcatagacac cgcgcgcgcc tggataagcg gcgttagaag atagctctcc
     6661 agaaactgga cgcagacgta aaggccgagc gcccagagcg ccatgtggaa gctctcgccg
     6721 cccgcgacca ggaggatcgg aatcgcgccg attatcgggc cgagataagg caggaagcac
     6781 gctaagccgg cgaacattgc gagcacgaag gcgatgggca caccgaggat cagaaggccg
     6841 acaaaggtga acccgccgat caccgacatg gtgatcgact ggccgatcag ccagcgccgg
     6901 agcaactcgc ccgtctcata cagaagctcc accaccctgc cgcgcttctc gggcggggcg
     6961 agccgcgcga ggaggttgac gtaagtgtgt gggtccgccg cgaggtagac gccgaagaac
     7021 acgacgatca atccggcact gaacaagccg aacagcgaaa tcgccacgga cgtggcgccg
     7081 gtggcgatgc cccactggct catgaagttt ccgagcagct gggcgagatc gatactcttt
     7141 agaaacgccg tgtgcttgac gaagctggaa agctcggtcg tgagtgtcgt cacaccggca
     7201 gccacgcttt ccgccagcaa tgcgatctga ttggcgagcg tcggtcccgc cgtccaggcg
     7261 gcaagcgcaa ggcctaacgc cacggcgatc gagacaatcg cgagcatcag gaggcggggc
     7321 aagccggtca attcgctaag gtcgtggctg gcgccgtaga gcacgatggc gagcaggatg
     7381 ccggcgaaca agagcagaat cgccgtttgc gcgtaccaca gcgcaaatgc gagcgcgatg
     7441 acggcgagta ccgccacggt gatcttcgcg gcctgaccag cactcatggg cgctggagag
     7501 cttgcggggc gtctggtgct gggctttgcg ggctcgcggg ccatggcggt cctgcttggc
     7561 tatggcgcgc ggcactgagg tgaaacgggc gcgcggaaga agggctcgtc tcaccaatct
     7621 acgtcaaagg aggtgggaag ggataggcgc tagtcgctga taccctcaaa gaactccttc
     7681 acccgggcaa agaatccggc cgaggccggg ctggtatccg ggttggaggc atcttcgaag
     7741 tctttcagca ggtcgcgctg cttgcgcgtg aggttcttcg gcgtctcgac ctcgacctga
     7801 atgtagaggt cgccttgcag cttcgagcgc agcaccggca tgcctttcga cttcaggcgg
     7861 aactgcttac cgctctcggt gccttcggga atcttcacgc ggcttcggcc gccgtcgatg
     7921 gtcggcacct cgacttgtcc gctaagtgcc gccgtcgtca tggcgatcgg cacacggcaa
     7981 aacagatcgg cgccgtcgcg ctggaagaac tcgtgcggct tgatcgacag gaagatgtac
     8041 aggtcgcccg gtgggccgcc gcgcaagccc gcttcgcctt cgccggcgag acggatacgc
     8101 gttccgtcct ccacgccgac gggaatgctg acggacagag tgcgttcgcg cataacgcgg
     8161 ccggcgccgt tacacgacgg gcacggcgtc tcgatcgtct caccacggcc ctggcaggcg
     8221 ggacaggtac gctcgatggt gaagaagccc tggctcgcgc ggacctttcc catgccgttg
     8281 caggtgcggc acgccgctgg caccgtgccg gctttcgcgc ccgatccttc gcaagaatcg
     8341 caagtgacgg atgtgggaac gcaaatctgc gccgtcttgc cttcataggc ttcggcgagc
     8401 tcgatctcca tgttgtagcg gaggtcggcg ccgcgctcgc ggcctgagcg ctgacgggga
     8461 cgccgcgcgc ccgcacccgc acccatgaac tcaccgaaca gatcgtcgaa catcgccgac
     8521 atggaggcgg agaaatccga gccgaagcca tgtccgggac cgccgaagcc gccgccgccg
     8581 ttctcgaatg cggcatggcc gaaccggtca tacgccgccc ggcgttgcgg gtccttcagg
     8641 gcctcgtagg cctcgttgac ttccttgaag cgtgtttcag cgctggggtc gccgttgcac
     8701 cggtcgggat ggcattcctt ggcgagcgaa cggaacgcgg acttgatctc aggatcgccc
     8761 gcgctgcgct caacgcccag aatgtcgtaa taacaacgtt taaccatcac ccctccggac
     8821 cttcgcgacc gctcatccta agcggcgcgc gggacctctt gttgggcgca agtggagccc
     8881 tctttcactc gagagctcca cgccgtgaaa taccgttacg ccgacttctt gtcgtcgtcg
     8941 tccttgacct cttcgaagtc gacatcgacg acatcttcgt cggggctgcc ggcttcggcg
     9001 gacggtccgc cttcttcttc accgccagca gcgccctggc tcgtctgata catcgcctcg
     9061 cctagtttca tggaggcttg ggcgaccgcg tcgaccttct gcgcgatggc ctcggggtct
     9121 tcgccctcgg cagcggtctt gagctcggcc acggccgctt cgatcgcggc tttgtcctcg
     9181 gctgaaacct tgtcgccgaa atcggcgagc atcttgtcgg tggagtgaac caacgcctcg
     9241 gcctggtttc gggcttccac cagctcgcgg cgcttcttgt cctcctcggc gtggctctcc
     9301 gcttccttga ccatgcggtc aatgtcagcg tcggcgaggc cgccggaggc ctggatgcgg
     9361 atcgactgtt ccttgccggt cgccttgtcg gttgccgaca cgttgacgat gccgttcgcg
     9421 tcgatgtcga atgtgacttc gatttgcggc acgccgcgcg gcgccggcgg aatgccaacg
     9481 agatcgaact gaccgagcat tttgttgtcc gccgccattt cgcgctcgcc ttggaagacg
     9541 cgaatggtca cggcgttctg gttgtcctcc gcggtggaga acacctggcc cttcttggtc
     9601 gggatcgtgg tgttgcgatc gatcaggcgc gtgaacacgc cgcccaaggt ctcgatgccg
     9661 agcgacagcg gcgtgacgtc gagcagcagg acgtccttga cgtcgccctg cagcacgccg
     9721 gcctggatgg cggcgccgat ggcgacgacc tcgtccgggt tcacgccctt atgcggctct
     9781 ttgccgaaga acgtcttcac ggtgtccgcg accttcggca tgcgcgtcat gccgccgacc
     9841 aggatcactt cggtaatctc gccgggcttg agaccggcat ccttcagcgc agcctcgcac
     9901 ggcgcgatgg tgcgcttgat cagatcctct accaggctct ccagcttggc gcgagtgagt
     9961 ttcatggtca gatgcttcgg gccggactga tcagccgtga tgaagggcag gttcacctcg
    10021 gtctgctgcg tcgacgacag ctcgatcttg gccttctcgg ccgcttcctt caggcgctga
    10081 agcgcgagct tgtccgagcg caggtcgatg ccttgctctt tcttaaactc atcggccagg
    10141 taatcgacga tgcgcatgtc gaaatcctcg ccgccgagga aggtgtcgcc attggtcgac
    10201 ttcacctcga acacgccgtc gccgatctcc aggatcgaaa cgtcgaatgt accgccaccc
    10261 agatcgtaga cggcgatggt ctggccttcc ttcttgtcaa gcccgtaggc gagcgctgcc
    10321 gccgtcggct cgttgatgat gcgcaggacc tcaaggccgg cgatcttgcc ggcatccttg
    10381 gtcgcttggc gctgggcgtc gttgaaatag gccgggacgg tgatgaccgc ttgctcaacg
    10441 gtctggccga gatgggactc ggcggtctcc ttcattttct gaaggatgta ggcggaaatc
    10501 tgagagggcg aatatttctt gccgtgagac tcgacccagg catcgccgtt gtcggcctcc
    10561 acgagtttgt aggggacgag cttctgatcc ttggcgaccg tggggtcgtc ccagcggcgg
    10621 ccgatcaggc gcttgatggc gaagaaggtg ttctcgggat tggtgaccgc ctggcgcttg
    10681 gccggcagtc caacgaggac ctcatcgtcg tcggtgaagg ccaccatcga tggggtcgtg
    10741 cgcataccct cggcattctc gatgactttg gggtttgccc cgtccatgac cgcgacgcac
    10801 gagttggtcg tgccgaggtc gattccaatc actttcgcca tttgcgagcc tctcttttct
    10861 ggcaaaccgt ccgggccctg gtgcggcacc cataaaaatc gcgcatcttt cggcgctgat
    10921 cggtccccaa tcgtttcgct gcccctggcc gccggccaag ggcggcaggg ctccgcggtt
    10981 atataggtgg caaccctatg gctcgcaagg agaagcgggg ttaacgcacc ggtctctgcc
    11041 aggtgtttca ggagacccgt ttccaacggc cctcctcttg ccccaccgca aggcgtggta
    11101 ggaccaagtg tcaggtatta cagcgaacca aggacggcgt gagacactcc ccgcaagccc
    11161 tcaatgaggc cggccaaagg gcccttttgg ccgagatcag ggctgtgatc gcgaaggccc
    11221 cgctcttcgt gcccaccatg ccccgctccg gcaatccgat gtccgtgcgc atgaccaacg
    11281 cagggccgct cggctgggtc accgacaaga cccatggcta ccgctatcag gccacgcacc
    11341 cggtcacggg cgagctttgg ccgccaatgc cgcaaacggt gttggatctc tgggaggcgc
    11401 tggcgggtca cccgcatccg ccggaggctt gcctcatcaa ctattacggg ggcgccgcca
    11461 agatggggct gcatcaggac aaggacgagg aggacttcga tgcgcccgtc ctctcggtct
    11521 cgcttgggga caccggcatc ttccgggtcg gcggcaagac gcgaaaaagc ccgacggaga
    11581 aatatgagct gaaatccggc gatgtcttcc tgctcggcgg cgaagaccgt ctcgcctatc
    11641 acggcatcga ccgcatcctg gccggtacgt cggatctctt ggaagaaggc ggccgcttca
    11701 acctcacctt gcggcgggtg acaaagccgg gatgagcgag gcgctagtcg ccgccgcccg
    11761 ggatgtcgtc gtcagccggg ccgcgctgtg gcgcctcagg ggccggctta gggccgcctt
    11821 ttgagaccgc aacgagggcc ggacgcagca cccggtcgcc gatcgtatag ccggcctgca
    11881 tgacctggac cacggtgcct tccggcactt cggtggttgg cacttcgtac atcgcctgct
    11941 gctgattggg atcgaagcgc tcgccgaggg gctcgagttt ggcgacgccg tgcttctcca
    12001 gcattttcag catatcgcgc tcggtgagct cgaccccctc cacgatgctc ttgagcgtgg
    12061 ggtcttgcgc cacggcgtct tcaggcacgt gctcaagggt ccgccgtaaa ttgtcgccga
    12121 tggcgagcac gtcgcgtgcg aaattggaga tggcgtagcg cgccgtgtct tccttctcgc
    12181 gctcggtgcg gcggcgcaaa ttctccatgt ccgccatggt gcgcaacagc ttgtcgcgca
    12241 tgtcggcatt ctcggtgagc agcccctcga ggtctccggc aagctccgcg ctcgcatccg
    12301 ggtcgacagc cgtctccggc gcatcaggct gggcctgatc ctcgcccgtc tcacgtggat
    12361 cggaattgtc gtccgccatc agacgcctcg acgcttgcgg ttcgaatggg aggcccggat
    12421 atcaaccgtg cgcgccaaaa aatcaagagg gtggcggcag ctcaaggaac caggcgcccg
    12481 agtagccgtg ccgtgtagtc aaccatgggg atgatgcgcg catagttgag ccgggtcggc
    12541 ccgatcacgc caaggacgcc cacgatcctc tgctccgaat cctcgaaggg cgagacgatc
    12601 agcgccgagc ccgacagcga gaacagcttg ttctccgagc cgatgaaaat acgaacgcca
    12661 tcggcgcttt cggcgaggcc cagaagctga atcaggtcct tcttggcatc gaggtcctcg
    12721 aacaggtgcc gcacgcgttc gaggtcctcc atcgccctga cgtcctccag caggtgcgcc
    12781 cgcccacaca cgatcaggct ctgacggtcg tccgcgtcgc ccgaccaagt ggcaagcccc
    12841 tccgtcacca cacgcgccgt gagctcgtcg agctgtgcgc gatgcgcctc gatctcccct
    12901 tcgatgcggt tgcgcgcctc cgccagtgtc agcccggcga tccgtgcatt ggtgtagttc
    12961 gtggcggcgc tgagcgccga aagtggcaac ccctcgggca gagtgacgat gcggttctcc
    13021 acgtcgccat cctccgcgac gagcaccgcc agggcctggc ccttgccgag atcgacgaac
    13081 tcgacttggc gcagcgtctt ggcctgctgc tgttccgcca ggacgacgcc ggcgcagcgc
    13141 gataggcccg acagcatttc acctgcctcg gcgagaaagt cctcgacccc gcgccgccgg
    13201 ccgccaccgg ccatgcgcgt ctcgatctcc ttgcgctccg cctcggtgat gtcgccgatc
    13261 tccaaaaggc cgtcgacgaa cagccgcaag cctgtttgcg ttggcaggcg tccggcgctc
    13321 gtatgtggcg cgtagaccag gccaatgctc tccagatcgg acatgacatt gcgcacggac
    13381 gcgggcgaca aggtcatggg tagcgcccgg ctcaaattgc gcgagccgac aggttcgccg
    13441 gtctcgagat atgtctcgac gatgcgcttg aagacttcgc gcgaacgctc gttgagttgg
    13501 cgcagattga cggcggcctc ggcggcggcc ttcgcgcctt tgacgtcggt atgacccatg
    13561 gtcctagaaa tctaggaggc gtccgcaccg gcgtcaaacg ggcacaccag cctggttaag
    13621 ccaagctaag caagccagtg agccttagtt cagtgcgccg gtcttcggcg caatctggga
    13681 attgagttcg tcgggcgcgc cgaaatgcgc gaagtagcgc gaattgatat gtttgaccgg
    13741 catgatgaca aatatgtcgg tcgtgccgaa ttggtgatcg atcaccgccc cgtcgccgac
    13801 aaaagcgccg acccgcaaat agcccttgat caaaggcggc aaggctttga gcgcagcacg
    13861 cgtattcacc gcatccttgg gcatcatgtt catgtcgacc cgcaaatggt cgtgcgcccg
    13921 cacacgccaa tcttcgggcg ccagcgagtt atggtgcagg aagctcaagg ccagcgcgtg
    13981 cgcggatggg tccgtccccg ggaagctggc gcagccgatc atcacgtcgg cgccgtgctc
    14041 gcgcacatac gaccagacgc cctgccacag aagctcgagc gtacgccggt tgcggtaggg
    14101 cttgagcacg caagagcggc cgagctcgat gaagctgtag tcggctttcg cccggatcag
    14161 cggggcgatg tcgtattcgc cttgcgtgta gaagccgtca tgcagttcgg cgatgtcttg
    14221 gcgcagcatg cgataggtgc cgacgacact cggccgccgc cagcctttct tgttgggatc
    14281 gccctggtcc atgacgagga ggtgatcgaa tatcggatcg aaggcatctt cgtcgcggcg
    14341 cgacagcata gcgagcgcgc ctggcgtggc cgacatctct tcgtagaaca ctttgtagcg
    14401 gaggcgctgt gcccgtcgga gttcgctctt cttttgcgcg agccggactt cgagggggcc
    14461 catgcggccg tagattctcg gctgctggaa gcgcccgcga aagccggcag gcgcacggcg
    14521 ccatcccatg aaggggagcc cgccaatctg gttcggcttg aagttcaatc cgggaaacat
    14581 ggctccacta cgctgtttgc ccaagggtcc ttgagttgtc ttcatcggca tgcctcagcc
    14641 cgtcactcga gttatctggg aacgcgccaa ttcggggatc caccctgggg tgccgccagc
    14701 gacggatgca atgcaaagcc tcgcgattgc ccgattcggc cataacacaa gccggttagc
    14761 aaaccaattc gaagtctatg tgacagcctg gggaaagcct ggagatgcgc ttggcgcgtg
    14821 tgtcaggcgg cgcctgcacc cgtctcttct gtctcgcgaa tgtgccaatg ggacaggagc
    14881 gccgccaggt cccgcgtttc gaagggcttg gcgaggtaat cgtcgaggcc ggccgcgaga
    14941 taagcagtgc ggtcctcggc gaaggcattg gcggtaagcg ccacgatggg cggccgccct
    15001 gcgccggggc ccgcatcgtc cgggtaaagc gcccgaatgc ggcgtgcggc ctcgacgccg
    15061 tccatgcccg gcatatggat atccgtcagc acgaggtcga accctcgcgc agccgttagg
    15121 gcagcgcggg cttcggcaat ggcatcggcg ccgttgcggg cacgcacgac gcgcgcgcca
    15181 cctttctcca ggacggtgcg ggcgagaagg gcattgatgt cgttgtcctc ggcaagaagg
    15241 acggagagtt cggcgagaca gtctggttcg tccaatgtcg gggcggggag cgcttcgggc
    15301 gtcgcggcgc atggcgcttg gtcgtgtgac gcttggtcct ctggcgcgtg gtcttgcgcc
    15361 gtgtcgccga agagttgggt cagcacggag agcggccgga ccggacgcac cagatagccg
    15421 ccaaagccgt gctcacggaa cttgggcatg tccgcacgct cactcggatc gatcacgacg
    15481 acggcgcgcg ggtgctcgca acctttcgct gcgacaagcg ctggaaggcg atccgcgccg
    15541 gcctcgatgc tcggcttgtc ggtcagcacc gcggtgaatg gcatgccttt gcgcgcggcg
    15601 tcactcaccg ttcgcgcggc gtcctcgggc ctggcgcgca ccacgcttgc acccatgccc
    15661 aacaggagtt cgcccacgag cgccgcctcc atactgccat tcagcacgat caggacatgt
    15721 tcggtgatct gggggccggg ccagctctcg ccgacccgct cgggtggcgt tggcgtttca
    15781 aacgggaggt ccgccgtaaa tgttgcgccg gcaccgggca cgctcgtcac gtcgatattg
    15841 ccgcccatct cgtccacgag ccgtttggag atggcaaggc caagccccgt tccgccatgc
    15901 tggcgggcgc ggccctgatc ggcctgctcg aattccgaga agatgcgtgt caaagcctcc
    15961 ggtggcacac cggggcctgt gtcgcgcacg gcgaagcgca tcaccgcgcg gtcgtcctgc
    16021 tgtgaaccag gggccatatt gagcgtgagt gcaacgccgc catgatcggt gaacttgatc
    16081 gcgttgccga ccagattcaa caggatctgc cggacacgca actcatcgcc gatgacagtc
    16141 tttggcaggt ccggcgcaac gaacccgccg atctcaaggc ccttgtcgcg cgcgcggggg
    16201 gccaacagct caacgacgtt ttgcgctgcg tctgcaatgt cgaacggcgc gttgtgcagc
    16261 tcgatctttc cggcctcgat tttggagaag tccagcactt cgtcgatgag cgacaaaagc
    16321 gtttttgccg atgtggagac ggcccgcgcg taggtgtgtt gctccgggga cagatccgtg
    16381 tcgagcaaga gaccggtcat gccgagaatg ccgctcatgg gcgtgcggat ctcgtggctc
    16441 accgaagcga gaaaacgcga cttggcgtcg cttgcgatca ttgcatcgtc gcgcgcttgc
    16501 gccagctcaa gctccgcggc ccgttgttcg gtgacgtcgc ggccgacgct ttgaacttcg
    16561 ctcatattgc cgtcatcgtc ggcgatggcg aagtcctccc acacgaacca gcgtgcgcct
    16621 gacacagtgg aaatctccac aacacggctg tgacgtccac cgcccttcat cgcggcggca
    16681 gtgctgtcgc agctgagtac aggaagctcg aaaacttgac cgagggcggc gtcccgcgac
    16741 gttccgaacg tttggcagaa cgcgtcgttg acgaaagaca gatggccgtc gacgtcacgg
    16801 cgcaggatca cgtcgccctg gtgatcgaga agatcgcggt agcgcgcttc gcgctcgcgg
    16861 acttcccatt gcagatcttt cagggcttcg aggttggagt ccagtcgtga tgcaaggctt
    16921 tcgacccgcg cgttgccagt cttgccgttt tgccggaggg ctaggatcaa aagcaccacg
    16981 cccgcgccca aggacgccag cgcgaatgtc tttatcgcgc cgagcagcac ggccgacagc
    17041 gcaacaagcg ccacggccaa gccgctcaag gcaacccgcg caccaatccc accggcgcaa
    17101 tccgcgccga agctctgcca agtcttacgc tgcactgttt gacgccgttt tgttatggcc
    17161 gccgggcgtt ttcgccctga cgtttcattg gcgccgagga tggcagagaa agcttgaaaa
    17221 aggctttagg ggatgcgttt aagcggcggc cttggcccgg cgcaaggtct cgccgcgata
    17281 gctcaaggcc tcggccacat ggattcgggc cacgttttcc tcgccgtcga gatcggcgag
    17341 cgtccgggcc actcgcagcg tccgataata gccgcgcgcg ctcaaagaca gcgcgttcgc
    17401 cgcatcgcgc aagagagaca gaccttggga atcgggcgcg gcgatctctt cgagcaaagc
    17461 cccatcacat tcggcattgg tgcgaatccg cggctgatca agcgctgcat agcgcgccgt
    17521 ttgtgccgcg cgcgcggccg cgacgcggac ccgaacctcg gcgcttcctt tctgaaggct
    17581 gcggcagcac cagatcgacg gcggaaacgg cgggcacttc gatctcaaga tcgatgcgaa
    17641 cgagcatcgg cccggacacc cgagtcaagt atgcatatgc cccgtcgctg caagaacaat
    17701 aagtgaacac tcactgcaaa ccgcacagaa gcaacgcatc tgtgctttgc tggccgccat
    17761 gcctcgggat ccccgcacgc tcgcgcttct tgcccacctc gccgcctatc tggtggccgt
    17821 ggccatcagc gccgccgtga atctgtggct tagccccgac aggctgtggt tcgtctggat
    17881 cgcggcaggc tgggggatcg gcgtcgccgc gcacgcgctc gccttctggc tggcgcatac
    17941 ccgccgccgc gagcgcgtct ttctcgaccc gcacgcgcgc ggcttcgcgg tccacgcctt
    18001 cgcctatgtg gcggtggtcg tgctgctgtt catcgtcaac gtgacagtca cgccaaaagt
    18061 ctggtggttc tattgggtcg cgctcggctg gggcgccggc gtcctcgccc atggctggtg
    18121 cgtgttcttc cgcaaatgtg cgcctagcgg ccggcggcca ccttctcctc gattacgtcc
    18181 cagatcattg cggcgatatc cacgccgtcg aactccttga cctggcggat gcccgtcggc
    18241 gaggtgacgt tgatctcggt gaggtagtcg ccgatcacgt cgatgccggc gaagatcagc
    18301 ccgcgcttct tgagttcagg tccgagccgt gcgcagatct catcctcgcg cggcgagagc
    18361 gtcgtcgctt tggcggttcc ccccacatgc aggttggagc gcgtctcgtc cgcggccggg
    18421 acgcggttaa tcgcgccggc cacttcgccg tcgactagga tgatgcgctt gtcgcccgcg
    18481 cgtacctccg gcaggtagcg ctgcaccatg atgggctcgc ggaaggtcag cccaagcaac
    18541 tcgatcagcg agttgaggtt ggtgtcgccc tcggcgatgc ggaagatcgc cgcgccgcca
    18601 ttgccgtaaa
//
LOCUS       DBNHVU010000017         3940 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1122285, whole genome shotgun sequence.
ACCESSION   DBNHVU010000017 DBNHVU010000000
VERSION     DBNHVU010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1122285"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..718)
                     /locus_tag="AC16Y7_00650"
     CDS             complement(281..718)
                     /locus_tag="AC16Y7_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5707918.1"
                     /translation="MPTVKHVLDKKGRDVHSIGPKASVFDALKVMDAHDIGALVVLDE
                     GRLVGLITERHYSREIILKGKTSPRTRVGDIMSTKVICALPQQSVEECMAVMTARAVR
                     HLPVLEHKQVVGVVSIGDMVKAVIDDQTFIIEQLEHYIHGHVH"
     gene            942..2498
                     /locus_tag="AC16Y7_00655"
     CDS             942..2498
                     /locus_tag="AC16Y7_00655"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5707919.1"
                     /translation="MAQATAKAGHDSRFWLVVAAACAGAFEVAYNTTAVMTALPAIKS
                     SLDLDVDTLQWVINIYMLASAVSLAAMGHFADTFGMMRIFAVGLCLFAIGSITIAFSG
                     DAAVLLAGRAFQGAGVAALMATSVALISVTAPPEKRAAAHGLWAAAVALGFALGPLIG
                     GTLTDVVSWRAIFGLDIAILAAVAALSFWVVRLGLVPRALDTEKRIDFLGIALLFVTL
                     VSFLYGLTSGPLFGWTSFQTLALFTAAILGGAGFVVRELRAADPLIQFGFFRHLDYVA
                     ATLGMFLNGVTQMGILYFTNLFLQAPEGLHFSASQAGLALLPFTSAMFVVSLILPRLI
                     APGSFRLPVTAGMLALVVGFWLMHGIDHQTRYSDIWWMLTILGIGVGFNWALLPRVGL
                     SVLPDANAGQGSGVLNTCMFAGLATGTAVGSVVASYIKRGLIDPVVDRVASTTPDLDS
                     LKVTLVHGSESQIARAMAQIPPDDARKLEGVMGSAFDSGFSGVMVMMAIAGLLGAVIC
                     FALISRRAAK"
     gene            2607..2738
                     /locus_tag="AC16Y7_00660"
                     /pseudo
     CDS             2607..2738
                     /locus_tag="AC16Y7_00660"
                     /inference="COORDINATES: protein motif:HMM:NF016230.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
     gene            complement(2883..3704)
                     /locus_tag="AC16Y7_00665"
     CDS             complement(2883..3704)
                     /locus_tag="AC16Y7_00665"
                     /inference="COORDINATES: protein motif:HMM:NF025700.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4396 domain-containing protein"
                     /protein_id="HIR5707920.1"
                     /translation="MVIDGVMLLWFILTAASVLFVAIDIRATPEHPVMKWAFVLFTAY
                     SGPIGAFLYVLGCREPLPGLHEQYVAARWRQALGSTMHCVAGDGIGILVGAVIGAVVA
                     LPMLADFALEYLLGFVFGWTIFQALFMGQMFGSYGRALKGTFTSELLSMNCLMGGMLP
                     VSALAFAGNEAAHDPTQALFWFRMSLALMVGAAFAYPMNWWLVAHHLKHGMVTVRPEG
                     EAAKPMPGMDKKMDMAAKSGSALPAPSPPVWVMAIVSFAVLATGVAVAIIFGGAT"
BASE COUNT          678 a         1207 c         1256 g          799 t
ORIGIN      
        1 gtcccgcggc cgagctgcgc acgggggcgg tcggataagg tgattgtcgt cgccattgtg
       61 ctgcccagcc cctagcgaac gcctcatgcg tgggcaaagc ccctttgagg cacccctaac
      121 tattgaccgg caatgcccga gggacgttga cctaagtcaa ctttccagcc gatggaccgg
      181 cttctatggg ctattcccga gcctcaaaag gcggggatgg cactggtaaa agggcggaaa
      241 taggacgcgc ggccccacgt gcattcacgg gtaaatgctc ctagtgcacg tgcccgtgga
      301 tgtagtgctc cagctgctcg atgatgaacg tctgatcgtc gattacggcc ttcaccatat
      361 cgccgattga tacgacgccg accacctgct tgtgctccag aacaggaagg tggcgtacgg
      421 cacgcgcagt catcacggcc atgcattcct cgaccgactg ctgcggcagc gcgcagatga
      481 ctttggtcga catgatgtct ccgacccgcg tccgcggcga ggtcttgccc ttcaagatga
      541 tctcacgtga gtagtggcgc tcggttatga ggccgacaag ccgaccctcg tccaacacga
      601 ccagcgctcc aatgtcgtgg gcgtccatga ctttgagggc atcgaacaca gaagcctttg
      661 ggccgatgct gtggacgtcg cggcccttct tatcaagcac atgtttcacg gtcggcatcg
      721 gacatcaccc cctttggacg atgatcgacc caaaagctta tcagcaaccc ttcggaccgc
      781 attgatttcg ctcaagacgc cttgggtttc ctctcgcgcc ggaaacaggg gctcgattcc
      841 cggggcgcca gcgctacaat ccgcgcgggc gcgccttccc aacgactttg agatagactg
      901 atcgcaacgc ccacgggggc ggcgcgaggg gggacttaca tatggcgcag gcgacggcca
      961 aggccggtca cgattccagg ttctggctgg tggttgcggc ggcctgcgcc ggcgccttcg
     1021 aggtggccta caacaccacc gcggtcatga cggccctgcc ggccatcaag tcgagcctgg
     1081 acctcgatgt cgacacgctc caatgggtga tcaatatcta tatgttggcg tcggcggttt
     1141 cgctcgcggc catgggccat ttcgccgaca cgttcggcat gatgcgcatt tttgccgtcg
     1201 ggctctgcct tttcgcgatc ggctcgatca cgatcgcttt ctctggcgat gccgccgttt
     1261 tgctcgcagg tcgcgcgttt cagggcgccg gcgttgcggc gctcatggcg acctcggtcg
     1321 cgctgatcag cgtcaccgcg ccgcccgaga agcgcgcggc cgcacatggc ctgtgggcgg
     1381 ctgcggtggc tctcgggttc gcgctcggtc cgttgattgg cggaactctg acagacgtgg
     1441 tcagttggcg cgcgatcttc gggctcgaca tcgccattct cgccgctgtc gcagctttga
     1501 gtttctgggt cgtccgctta gggctggttc cgcgtgcgct cgataccgaa aagcggatag
     1561 actttctcgg catcgcgctc ctttttgtga cgctcgtgtc ctttctctat ggcctcacca
     1621 gcggcccgct gttcggctgg acatcgtttc agactttggc gctcttcacg gcggccatac
     1681 tgggcggcgc tgggtttgtt gtgcgggaac tccgggctgc cgaccctctc atccagttcg
     1741 gctttttccg ccatttggac tacgtggccg caacgctagg catgttcttg aacggcgtga
     1801 cccagatggg cattctgtat ttcaccaatc ttttcctgca ggcgccggaa ggtttgcatt
     1861 tcagcgcctc gcaagccggg cttgccctgc ttccctttac gtcggcgatg ttcgtggtgt
     1921 cgcttatcct gccgcggctg attgcgcccg gcagtttccg cttgccggtc accgccggaa
     1981 tgctggcgct ggtggtggga ttttggctga tgcacggcat cgaccatcag acacgctata
     2041 gcgacatttg gtggatgctg acgatcctcg gcattggcgt cgggttcaac tgggctctcc
     2101 tgccgcgggt gggactgagt gttctgcccg atgcgaacgc aggccaaggg tctggcgttc
     2161 tcaacacatg catgttcgca ggcctcgcca ccgggacggc cgtgggcagt gtcgtggcgt
     2221 catatatcaa gcgcgggctt atcgatcctg tcgtcgaccg cgtcgcctca acgaccccgg
     2281 atctcgattc actgaaggtc acgctcgtcc atggctcgga aagtcagatc gcacgcgcca
     2341 tggcgcaaat tccgcccgac gatgcacgga agcttgaagg ggtcatgggg agcgccttcg
     2401 acagcgggtt ttccggcgtc atggtgatga tggcgatcgc ggggctgctc ggcgctgtca
     2461 tttgcttcgc cctgatcagc cgccgggctg ctaagtagcc ggcagcttgg ctggctggtg
     2521 ggcggacgag gccctgatcc gccgcgggca cggacttaca agcccttcgc cgagcacaat
     2581 tcctagagcc tcgcccaacg gcttcaattt gtgcctgctt cggcatgggg ctgtcggcta
     2641 ttcaggtgtt gatcgcaagc ggcgatgctg tctccgtgga ggcggccggc gaacatctca
     2701 aggcgggcac gaattgcggg tcctgccaac tggaaatcaa gaggctcatc aggctactaa
     2761 gggggaacgc gtcctcgcag gcgcaacggg gtaaatcggg cactgccaat cagaccgtta
     2821 ctgctggtga ctttcctaat cgcgttggat cgcggcgcgc actcggcgtg cgtgggctgt
     2881 gcctaggtcg ctccgccgaa gatgattgcg accgccacgc cggtggccaa cacggcgaac
     2941 gacacgatgg ccatgaccca gactggcggg gagggggcgg gcagggccga gccgcttttc
     3001 gcggccatat ccatcttctt gtccatgccg ggcatcggtt ttgccgcttc gccttcgggc
     3061 cgcaccgtga ccatgccgtg tttgaggtgg tgcgccacga gccaccagtt catcgggtag
     3121 gcgaacgcgg cgccgaccat cagcgccagc gacatgcgga accagaacag ggcttgtgtg
     3181 ggatcgtggg cagcctcatt cccggcgaag gcgagcgcgg agaccggcag catgccgccc
     3241 atgaggcagt tcatcgagag cagctcggac gtgaacgtgc ctttcagcgc gcggccgtag
     3301 gagccgaaca tctggcccat gaacaaggcc tggaagatgg tccagccgaa gacgaagccg
     3361 agcaggtatt ccagcgcgaa gtcggcgagc atgggcagcg cgacaaccgc gccgatcacc
     3421 gcgccgacca agatgccgat gccgtcgcca gcgacgcagt gcatggtgga gccgagcgct
     3481 tggcgccagc gggcggcgac atattgctcg tgcaggccgg gcaggggctc ccggcagcca
     3541 agcacataaa gaaaggcgcc gatcggcccc gaataggcgg tgaagaggac gaaggcccat
     3601 ttcatcaccg ggtgctcggg cgtggcgcgg atgtcgatgg ccacgaacag cacggatgcc
     3661 gcggtcagga tgaaccagag cagcatgacg ccgtcgatga ccatggatcc cccttaatag
     3721 cccctgattc gtcccgaaat cagcccagtt taacgcctcc ccaggtcacc gagaaatggc
     3781 ctggaagggc gaacccgagg gctgaaagtg cccaattctt cggcaaatcg gccgggcagc
     3841 tggccaaggc cgtgccaatt gtctgccctc tgttgcttct ctgctctatc ggcgagggat
     3901 tgatgggcgt aggcttatgt cagtcttgcg aatcagtttt
//
LOCUS       DBNHVU010000018         6787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_8439, whole genome shotgun sequence.
ACCESSION   DBNHVU010000018 DBNHVU010000000
VERSION     DBNHVU010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6787
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_8439"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /gene="tsf"
                     /locus_tag="AC16Y7_00670"
     CDS             <1..586
                     /gene="tsf"
                     /locus_tag="AC16Y7_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006925393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR5707921.1"
                     /translation="GKTKTNVAETLKEMVGSIGENMTLRRTAYLSAKDGVVANYMHNQ
                     AAPGLGKIGVIVALESTGDADALKAIGKQVAMHIAAASPQAVDTDSLDPEVVERERAV
                     LTEQAKESGKPPEVVDKMVEGRLRKFYQDVVLLQQAFVHDPDNTVAKALEAAGKEAGA
                     PIKITGFYRFALGEGIDKGESDFAAEVAAAASGS"
     gene            665..1411
                     /gene="pyrH"
                     /locus_tag="AC16Y7_00675"
     CDS             665..1411
                     /gene="pyrH"
                     /locus_tag="AC16Y7_00675"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR5707922.1"
                     /translation="MDKSPSPSKLRYGRVLLKLSGEALAGDKGYGIDLDVVEPIARDI
                     AEAADMGAEICLVVGGGNIYRGLMAAESGMDRARADYMGMLATVMNALALQTAIERVG
                     PTARVLSAIPMPTVCEPYIRDKALDHLEKGRVVIFAGGTGNPFFTTDTAAALRAAEME
                     CRALYKATKVDGVYSADPVKVPDAKRYVRLSYDDVLNQDLKIMDGAAIALARDNRIPV
                     IVFSIKSQGSLALELQGQGASTTIDDGRGA"
     gene            1500..2057
                     /gene="frr"
                     /locus_tag="AC16Y7_00680"
     CDS             1500..2057
                     /gene="frr"
                     /locus_tag="AC16Y7_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008546529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR5707923.1"
                     /translation="MSSFDLDEIERRMRGALNVLKQEFGGLRTGRASASLLDPIMVSA
                     YGNPMPLNQVATVNVPEPRMITVQVWDKSQINAVDKAIRESDLGLNPVVEGQLLRLPI
                     PELNQERRQELVKVAHKYAEQARVAVRNVRRDGMEHLKKAEKDGDMGKDEHHTLGTKV
                     QDLTDKVVKEIDGALATKEAEITQV"
     gene            2118..2861
                     /locus_tag="AC16Y7_00685"
     CDS             2118..2861
                     /locus_tag="AC16Y7_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIR5707924.1"
                     /translation="MDNKWGDADRPVARHVAIIMDGNGRWAAERGLPRVEGHRQGVES
                     VRRTVEAAMEQGITHLTLFSFSSENWSRPKQEINDLFGLLRRFVRRDLAELHKNGVNI
                     RVIGARTNLDDDILRMLNDAVELTKDNTALNLTIAFNYGSRDEIARAAQRIAEDAKEG
                     RVAPEDVTEERLADYLDTAGLPDPDLLIRTSGELRLSNFLLWQLAYAEFVFVDVFWPD
                     FTKEVFEEAVAEYQRRSRRFGGTSARSTA"
     gene            2858..3721
                     /locus_tag="AC16Y7_00690"
     CDS             2858..3721
                     /locus_tag="AC16Y7_00690"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR5707925.1"
                     /translation="MTPTQTPSDKHPKDPAPQPMPELLRRIISALVLGAIALAAVIAS
                     PWSFLVLVIVAGGLLAWEWGSLTRGSGFDGTALVHAIAVIAIAVLVAFSRPDLAILIL
                     GLASVALFFAAPDRRSGAWALAGLLYVALPVWSLIWLRSDPNYGLVAILFLLAIAWTT
                     DTASYFGGRSIGGPKLAPRISPKKTWAGFFTGVLTAAAVGYAIALYLGGTSPWILALV
                     GAGLALACQLGDLLESAVKRHFGVKDMSQLIPGHGGLFDRIDSLLLAAVVAGLIALRN
                     PAAPGAGLLIW"
     gene            3794..4948
                     /gene="dxr"
                     /locus_tag="AC16Y7_00695"
     CDS             3794..4948
                     /gene="dxr"
                     /locus_tag="AC16Y7_00695"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR5707926.1"
                     /translation="MTVLGATGSIGESTLDLVGRDPDAYEVIALTGCKNATRLAELAI
                     KHRAKLAVVADPDCYADLQEALVGSGIAAASGEKALLEAASRPADWIMAAIVGAAGLK
                     PTLRAVKQGTATALANKECLVSAGALFMDEVARHDTTLLPVDSEHSAALQAMTGTPPE
                     SIDRVCLTASGGPFRTWDLQQMADAEPAQALKHPNWSMGAKVTIDSATLMNKGLELLE
                     AHHLFALAPKKLDVLVHPQSIVHCLVYMRDGAVLAQMSCPDMRTPIAYSLAWPNRMNV
                     PDMRLDLAKVGTLTFESPDPEKFPALRLAKEVLAAGGSAGTVLNAANEVAVEAFLDNR
                     LGFLAIAGLVETTLAGSPDLIALSPQTVGEILEIDEEARRRARVSLPRIA"
     gene            4999..6141
                     /gene="rseP"
                     /locus_tag="AC16Y7_00700"
     CDS             4999..6141
                     /gene="rseP"
                     /locus_tag="AC16Y7_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR5707927.1"
                     /translation="MDTINLLSGFGSSFLSYLIPFLFILTLVVFIHELGHFLVARWCG
                     VGVKAFSIGFGPEIFGFYDRHGTRWRVAWIPLGGYVKFVDDENAASAGQTSLDELSPE
                     DRAKSFQGKPLVQRAAIVAAGPIANFILAIVIFTFMFSVYGERITAPKVDAVSPNSAA
                     ERAGFKPGDEVVSIDGDEIDNFATMQRLVSMSPDQDLRFVVDRGGAKVELTATPERKE
                     ITDRFGNTLKIGLLGIQRSASPDDWSWERHDPATALVMAVQECYFVISRSLGYLYDVI
                     MGREDADQLGGPIRIAQISGQVATAGFVALLNLAAIISVSIGLINLFPIPMLDGGHLL
                     FYGIEALRGKPLSESTQEIGFRIGLAFVLMLMIFATWNDLIHLKFL"
     gene            6306..>6787
                     /locus_tag="AC16Y7_00705"
     CDS             6306..>6787
                     /locus_tag="AC16Y7_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HIR5707928.1"
                     /translation="MVRWTLERGLLAALLVLSSVPLSLGVSACFGDLYASAQGAVIRS
                     IVVEGNRRVEPETVRSYLTFSSGDAYDPGEVNQSLKTLFATGLFQDVRIRRSGSTIIV
                     IVVENPIVSRVAFEGNEDIDDETLTPEVQLKPRAVYTRARVQADVQRILIVYRRKGHY
                     S"
BASE COUNT         1304 a         2165 c         2083 g         1235 t
ORIGIN      
        1 cggcaagacc aagacgaacg tcgcagaaac cctgaaggag atggtgggct ccatcggtga
       61 gaacatgacg ctccgccgga cggcgtacct ctcggccaag gacggcgtgg tcgcgaacta
      121 tatgcacaac caggccgcac cgggcctcgg caagatcggc gtcatcgtgg cgctcgaatc
      181 taccggtgac gccgatgcgc tcaaggcaat cggcaagcag gtggccatgc atattgcagc
      241 cgccagcccg caggccgtgg acaccgacag tctcgatccg gaggtcgtcg aacgggagcg
      301 cgccgtgctg accgagcagg ccaaggaatc gggcaagccg cccgaggtcg tggacaagat
      361 ggtcgagggc cgtttgcgca agttctacca agacgtcgtc ctgcttcagc aggcattcgt
      421 ccacgatccg gataacaccg tcgccaaggc cctcgaggct gcgggcaagg aggccggggc
      481 ccccatcaaa atcaccgggt tctaccggtt cgcgctcggc gaggggatcg acaagggcga
      541 gagcgatttc gccgcagagg ttgccgcggc tgcatcgggc tcctagggtc ggtgacaatc
      601 tcgggatgga ttcgggtctg agacttgacc tgaggtcacc gcatcgccac gggagttcgc
      661 gctgatggac aaatcgcctt cgccgtccaa actccgttac ggacgcgttc tcttgaagct
      721 gtcgggcgaa gcgctcgcgg gcgacaaggg ttatggcatc gatcttgacg tggtcgagcc
      781 gattgccagg gacatcgccg aagcggccga catgggcgcc gagatctgcc tcgtcgtggg
      841 cggcggcaat atctatcgcg gattgatggc tgccgagagc ggcatggacc gcgcccgcgc
      901 cgactatatg ggcatgctgg cgacggtgat gaacgctctc gcccttcaga ccgctatcga
      961 acgggtaggc ccaacagcac gggtgctctc ggcgatccca atgcctacgg tgtgcgagcc
     1021 atacattcgc gacaaggccc tcgaccatct ggagaagggc cgggtggtaa tctttgccgg
     1081 cggcacggga aatcccttct tcaccacgga caccgccgcg gctctgcggg ccgccgaaat
     1141 ggaatgccgg gccctctata aggccaccaa ggtggatggg gtctacagcg ccgacccggt
     1201 caaggtcccg gatgcaaaac gctatgtccg cttaagttac gacgatgtgc taaaccagga
     1261 tctgaagatc atggatggcg ccgcgatcgc ccttgcccgg gacaaccgga ttcccgtaat
     1321 cgtgttttca atcaagagcc aaggcagctt ggctcttgag ttgcaagggc agggcgcctc
     1381 gaccaccata gacgatggaa ggggcgccta atggacgctc ggaaaacgcc aacagggctc
     1441 aagcccccac gccgcgccca accggaaccg gcgtaagggg cggggcagga aggcaacgaa
     1501 tgtctagctt cgacttggac gagatcgaac gacgcatgcg tggtgcgctc aacgttctca
     1561 aacaggaatt tggcggtctg cgtacaggcc gcgccagcgc aagcctgctc gaccccatca
     1621 tggtgtcggc ctacggcaac cccatgccgt tgaaccaagt ggcgacggtc aatgtgccgg
     1681 agccgcgcat gatcacggtg caggtctggg acaagagcca gatcaatgcg gtcgacaagg
     1741 cgatccggga gtcggacctc ggtctcaacc ccgtcgtcga ggggcagctc ctgcgcctgc
     1801 cgatccccga gctcaaccag gagcgccgtc aggaactcgt caaagttgcc cataaatatg
     1861 ccgaacaggc acgcgtcgcc gtgcgcaatg tccggcgcga cggcatggaa cacctcaaaa
     1921 aggccgagaa agatggtgac atgggcaaag acgaacacca tacactagga acgaaggttc
     1981 aggatttgac cgataaggtg gtcaaagaga tcgacggggc cctggccacc aaggaagccg
     2041 aaatcacgca ggtctgaggc catgcgcgcg attccctttc aagagacgct ttggatacgc
     2101 cgaccgtgac acttgcgatg gacaacaaat ggggtgacgc cgatcggccg gttgcgcgcc
     2161 atgtcgccat catcatggac ggcaatggcc ggtgggccgc cgagcgcggt ctgccgcgtg
     2221 tcgagggtca ccgccaaggc gtcgagagcg tccggcgtac cgtcgaggcg gccatggagc
     2281 agggcatcac ccacctgacc ctgttcagct tctcctcgga gaactggtcg cgccccaagc
     2341 aagagatcaa cgacctgttc gggcttcttc ggcgctttgt ccgccgcgac ctcgccgaac
     2401 tgcataagaa cggcgtcaac atccgtgtca tcggcgcgcg caccaacctc gacgacgaca
     2461 tcctgcgcat gctcaacgat gccgtcgaac tcaccaagga caacaccgcg ctcaacctga
     2521 ccatcgcctt caactacggg tcgcgcgatg agatcgcccg cgccgcgcaa cgcattgccg
     2581 aggacgctaa agaaggccgc gtcgcaccgg aggacgtgac cgaggagcgc ttggccgact
     2641 atctcgatac ggccggcctg ccagatcccg accttctgat ccgcaccagc ggcgagttgc
     2701 gcttgtccaa cttcctattg tggcaattgg cctacgccga attcgtcttc gtcgacgtct
     2761 tctggcccga cttcaccaag gaagtgttcg aagaggccgt tgccgagtac cagcgtcgca
     2821 gccgccgctt cggtggaacc tcggcgagat cgaccgcgtg acgccaaccc aaacgccttc
     2881 cgacaagcac ccgaaagacc ccgcaccgca acccatgcct gagctcttgc gccggatcat
     2941 ctcggccttg gtgctggggg ccattgcgct tgcagccgtc atcgccagcc cgtggagctt
     3001 tcttgttctc gtgatcgttg ccggcggact cctggcctgg gagtggggca gccttacccg
     3061 cggcagcggc ttcgacggta ccgccctggt gcacgccatc gcggtcatcg cgatcgcggt
     3121 tctcgttgcc ttcagtcgcc cggatctggc gatccttatt cttggcctgg cgagcgttgc
     3181 gctattcttt gccgcccctg accggcgaag cggtgcctgg gctttggccg ggctgctcta
     3241 tgtggcactc ccggtttgga gcctgatctg gctgcgcagc gaccccaatt acggccttgt
     3301 cgccatcctc tttctcctgg ccatagcctg gaccacggac acggcctcct attttggcgg
     3361 ccgcagcatc ggcggcccga aattggcgcc ccgcatctcg ccgaagaaaa catgggccgg
     3421 cttcttcacc ggcgttctga cagcggcggc cgtcggctat gctattgcgc tgtatctcgg
     3481 cggcacgtct ccatggattt tggcgctggt gggcgccggt ctcgcgctcg cctgccagct
     3541 gggagacctg ctagaaagtg ccgtgaagcg gcatttcggt gtgaaggaca tgagccaact
     3601 cattccgggt catggcggac tcttcgaccg gatcgacagc ctcctcctgg cggcggtcgt
     3661 ggcgggcctc atcgccttgc gcaatccggc cgcgcccggt gccggccttc tcatctggta
     3721 aggacgcgcc ctgttaccgg cacagacatc ctcaagcccc gagcccacgc aaaacgccgc
     3781 acggccaaag cggatcaccg tgctcggcgc caccggctcg atcggcgaaa gcacgctcga
     3841 cctcgtcggc cgcgatccgg acgcctatga ggtgatcgcg ctaacgggct gcaagaacgc
     3901 gacccgcctt gccgagctcg ccatcaagca tcgcgctaag ctcgccgtcg tcgccgaccc
     3961 cgattgctac gcggacttgc aggaggcgct ggtgggctca gggatcgccg cggcgagcgg
     4021 cgagaaggcg ttgctcgaag cggcaagccg tccggctgac tggatcatgg ccgcgatcgt
     4081 cggcgcggcg ggactgaaac cgacattgcg cgcggtaaag caaggcacgg ccacggcact
     4141 tgccaacaag gaatgcctcg tctcggccgg ggccttgttt atggacgagg tggcgcgcca
     4201 cgacacgacg ctgcttccag tcgattccga acactcagcg gcgctgcaag cgatgacggg
     4261 cacgccgccg gagagcattg accgcgtctg tctcaccgcc tcgggcggac cgttccgcac
     4321 gtgggacttg caacagatgg ccgacgcgga acccgcgcaa gccttgaagc atcccaattg
     4381 gtccatgggc gccaaggtca ccatcgattc cgcgacgctc atgaacaagg ggctggagct
     4441 cttggaggcc catcaccttt tcgcgctcgc acccaaaaag ctcgacgtgc tggtccaccc
     4501 gcagtccatt gtccactgcc tcgtctatat gcgcgacggc gcggtcctgg cgcagatgag
     4561 ttgcccggat atgcgcacgc caatcgccta cagcctggcg tggccgaacc gcatgaacgt
     4621 tccggacatg cgcctcgacc tcgcgaaggt cggcacgctg acctttgaga gccccgatcc
     4681 ggagaaattc ccggccttgc gtctcgcaaa ggaagttctc gccgcgggcg gcagcgccgg
     4741 aacggtgctc aacgcagcca atgaagtggc cgttgaggcc tttctcgaca atcgcctcgg
     4801 cttcctggcc atcgccgggt tggtggaaac cacgctcgcc ggcagtccgg acctaatcgc
     4861 ccttagcccg cagaccgttg gcgaaattct tgagatcgac gaggaggccc gccggcgcgc
     4921 acgggtctcc ctcccacgga ttgcataagc ctgacctgcg ggctaagaac ggacaaatta
     4981 ttgaggtaac tggcacttat ggacacgata aatctgcttt ccggctttgg cagctccttc
     5041 ctcagctacc tcattccatt tctcttcatc ctgaccctgg tggtcttcat ccacgagctc
     5101 ggccactttc ttgtggcccg ttggtgcggt gtcggcgtca aggcgttctc catcggcttc
     5161 gggccggaga tcttcgggtt ctacgaccgg cacggcacac gttggcgggt ggcatggata
     5221 cccctcggcg gctacgtgaa gttcgtcgac gacgagaacg ccgcaagcgc aggccagacg
     5281 tcgttggacg aactgtcgcc ggaagatcgc gccaagagct ttcaaggcaa accgctcgtc
     5341 cagcgcgccg ccatcgtcgc cgccggacca atcgccaatt tcatcctggc gattgtgatc
     5401 ttcacgttca tgttcagcgt ctatggcgag cgcatcaccg cgccgaaagt ggacgccgtc
     5461 tcaccgaaca gcgcggcgga gcgggcaggc ttcaaacccg gtgacgaggt cgtcagcatc
     5521 gacggcgatg agatcgacaa cttcgccaca atgcagcgcc ttgtctccat gagccccgat
     5581 caggatcttc gcttcgtggt cgaccggggc ggcgccaagg tcgagctcac ggcgacgccg
     5641 gagcgcaaag agatcaccga ccgattcggc aacacgctga agatcgggct gctcggcatc
     5701 cagcgcagcg catcgccgga cgattggtcc tgggagcggc acgatccggc cacggccttg
     5761 gtcatggcgg ttcaggagtg ctacttcgtc atttcccggt ccctgggcta cctctacgac
     5821 gtcatcatgg gacgggaaga cgccgaccag ctcggcggac ccatacggat cgcccaaata
     5881 tcggggcagg tggccacggc gggatttgtt gcgctcctga acctcgcggc catcatttcc
     5941 gtcagtattg gtctaataaa cctgttccca ataccaatgc tcgatggtgg acatcttctg
     6001 ttttacggca tcgaggctct cagaggaaaa ccgctcagtg agtccacaca agagatcggt
     6061 ttccgtattg gcctagcatt cgttctcatg ctaatgatct tcgcgacctg gaacgacttg
     6121 attcacttga agtttttgtg atctcaggtt gtagaggcgc acgcttagcg gggaccccga
     6181 ggtccccgta tggttaacgg gcgacaccaa aaatcggcca caaacaaatg gccagttgcg
     6241 gtctcaaggc atttgccgcc aaggcttttt gccaacgggg tcggcttgag gggaagacac
     6301 cagtaatggt gcggtggacg ttggaacgag ggctgctcgc ggccctcctc gtgctgagta
     6361 gcgtcccgct ttcgctgggc gtcagcgcct gttttggcga cctctatgcg tccgcccaag
     6421 gcgccgtcat ccgatccatc gttgttgaag gcaaccggcg cgtagagccg gagacggtcc
     6481 ggtcctacct gacgttctca tcgggcgacg cgtacgatcc cggcgaggtc aatcaatccc
     6541 tcaagacgct gttcgctacc ggcctgttcc aggacgtccg cattcgtcgc agcggctcga
     6601 ccataattgt catcgtcgtt gaaaacccga tcgtcagccg tgttgcgttc gaagggaacg
     6661 aggacattga cgacgagacg ctcactcctg aagtccagct caaaccacgc gccgtctata
     6721 cgcgagcccg agtccaggcg gatgtgcagc gcatcctgat tgtttatcgg cgcaagggcc
     6781 attactc
//
LOCUS       DBNHVU010000019         5273 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_601820, whole genome shotgun sequence.
ACCESSION   DBNHVU010000019 DBNHVU010000000
VERSION     DBNHVU010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5273)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5273)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5273
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_601820"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1864)
                     /locus_tag="AC16Y7_00710"
     CDS             complement(<1..1864)
                     /locus_tag="AC16Y7_00710"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR5707929.1"
                     /translation="MTRQYELVERVLSYQPNANEAMLNRAYVYAMKAHGNQKRASGAP
                     YFSHPLEVAAILAEMRLDDATIATALLHDTIEDTTVTRAEIDTLFGAEIGTLVDGLTK
                     IKKLDLVTKKAAQAENFRKLLLAISSDIRVLLVKFADRLHNMRTLKHMKPAKRKIIAE
                     ETMDIYAPLAGRMGMQWLRDELEDHAFRWLNPEAYATVVERLKILRERNQGLIDEIQD
                     ELTTKLKQARVKGIASGREKKPYAIWSKMERKQISLEQLSDIYGFRIVVPEVADCYHA
                     LSVVHTTWHAVPGRFKDYISNPKQNDYQSIHTTIVGPRHQRVELQIRTEKMHQVAEYG
                     VAAHGLYKDLNGANGKGKVPSQDSSAYRWLRHLVGMLLEGDNPEEFLEHTKLELFQDQ
                     VFCFTPKGRLIALPRGANCIDFAYAVHTDVGNTCVGAKINGRSMPLVSALRNGDEVEI
                     VCSEAQAPPAAWQSLAVTGKARSAIRRATRDSVRAQYGKLGREILDHAFQRRGKTFNE
                     ETIGAALGRLSQKTIEDVMAAVGRGELASADVVRAVFPEDAPPEPPKRRREVKRNDEG
                     WFGLGNKVRGLKFRWPGSSRERPEGASGIPIRGLRGDLPVNFADGGAVPGDRIVG"
     gene            complement(1967..2377)
                     /gene="rpoZ"
                     /locus_tag="AC16Y7_00715"
     CDS             complement(1967..2377)
                     /gene="rpoZ"
                     /locus_tag="AC16Y7_00715"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR5707930.1"
                     /translation="MARVTVEDCIDKIQNRFELVLLAAHRARTISAGAQIAVQRDNDK
                     NPVVALREIADKKILPDDLKEDFIHSMQKYVEVDEPEAEAVPMLSSETRMPVLGQDDQ
                     STDTQIDRMSEEELLRRMATQAPSDGSNAGPRAR"
     gene            complement(2466..3011)
                     /gene="folK"
                     /locus_tag="AC16Y7_00720"
     CDS             complement(2466..3011)
                     /gene="folK"
                     /locus_tag="AC16Y7_00720"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR5707931.1"
                     /translation="MVAKAQVLVGLGSNSPGPWGTPAETITRALRELQDAGVTVISVS
                     PYYRTAAMGRARQPAYVNAAAAIDTALPPEALLRVLKAIERASGRRGGSPWGPRTLDI
                     DILDYKGLVRHWRGKHPRFARAGPRPLVLPHPWIEKRPFVLRPLLDVAPFWRHPVTRR
                     SAQEVWRSVAWSREGQVIGKR"
     gene            3106..3723
                     /locus_tag="AC16Y7_00725"
     CDS             3106..3723
                     /locus_tag="AC16Y7_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LabA-like NYN domain-containing protein"
                     /protein_id="HIR5707932.1"
                     /translation="MNVYPTERIALFIDGSNLYAAAKSLSFDIDYKRLLTLFGLKGQL
                     VRALYYTALSEDQEYSSIRPLIDWLDYNGYTMVTKPTKEFVDAAGRRKVKGNMDIELA
                     VDAMELAEHLDHLVIFSGDGDFRSLVEALQYKGKRVSVVSTLTTNPPMVADELRRQAD
                     QFIDLASLQNEIGRDPSQRTQREQRQRPDGEPPTDEFEPGDLIET"
     gene            3777..4466
                     /locus_tag="AC16Y7_00730"
     CDS             3777..4466
                     /locus_tag="AC16Y7_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR5707933.1"
                     /translation="MTLIASPANQPEPASDCPLCPRLVEFRHDNQTAHPDWFNGAVPS
                     FGPETARLLIVGLAPGLKGANRTRRPFLGDHAGDLLYPTLLKVGLARGTHDVETPEAL
                     QLIDCMITNAVRCVPPQNKPTSQEVKTCRPFLIGRMEALPDLRALLAIGRIAHDSLMD
                     VFGLKKRDFPFSHGARHTLPGGLILFDTFHCSRLNTNTGRLTPEMFEAVVGAAAASLD
                     EASLSEAALKD"
     gene            4529..4936
                     /locus_tag="AC16Y7_00735"
     CDS             4529..4936
                     /locus_tag="AC16Y7_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HIR5707934.1"
                     /translation="MAGIEISPTKVGFVILKAREYGAKVGAWDDSATSDHDAESILED
                     FADDSTVAELKEFIHDLNEDEQVSLVALAWIGRGTFSPEEVSEAIDTARAERTTRTED
                     YLLGIPLLSDYLEEGLDKLGYSVEEAEDGAMGD"
     gene            complement(4957..>5273)
                     /locus_tag="AC16Y7_00740"
     CDS             complement(4957..>5273)
                     /locus_tag="AC16Y7_00740"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HIR5707935.1"
                     /translation="ERFGALSPRFEALDVRLFGLSLQEVGYQQELVTRLGLPFPLLSD
                     TGEEFSGALALPSFATGGQTYLKRLTLVVRDGAIEVPFYPVPDPATHADEVLAWLEKA
                     TR"
BASE COUNT          954 a         1685 c         1603 g         1031 t
ORIGIN      
        1 tgccgacaat gcgatcaccc ggcaccgcgc cgccgtcggc gaagttcacc ggaaggtcgc
       61 cgcgaagacc gcggatgggg atgccgctgg cgccttcggg acgctcgcgg ctcgacccgg
      121 gccaacgaaa cttgaggccc ctcaccttgt tgccgaggcc gaaccagcct tcgtcgttgc
      181 gcttgacctc gcggcgccgc ttgggcggct caggcggcgc gtcctcgggg aataccgcgc
      241 gcacgacatc ggcagaggcg agctcgccgc ggccgacagc ggccatgaca tcttcgatgg
      301 ttttctgtga cagacggccg agggccgccc cgatcgtctc ttcattgaaa gtcttgccgc
      361 ggcgctgaaa ggcatggtcg agaatctcgc ggccgagctt gccgtattgc gcgcggaccg
      421 aatcgcgcgt tgcgcgcctg atggccgagc gcgccttgcc cgtcacagcg aggctttgcc
      481 atgcggcggg cggcgcttgc gcttccgagc agacgatctc gacttcgtcg ccgttgcgca
      541 gcgccgacac aagcggcatc gaccggccgt tgatcttggc gccgacgcat gtattgccga
      601 catcggtatg gaccgcatag gcgaagtcga tgcagttcgc gccgcgtggc agcgcaatca
      661 agcgcccctt cggcgtgaag cagaacacct ggtcctggaa cagctcaagc ttggtgtgct
      721 cgaggaattc ttccgggtta tcgccctcga gcaacatgcc tacgagatgg cgcagccatc
      781 gataggcgct ggaatcttgc gaaggcacct tgcctttgcc attggcgccg ttgaggtcct
      841 tgtagagacc gtgtgccgcc acaccgtact cggcgacctg atgcatcttt tcggtgcgga
      901 tctgcaactc gacgcgctgg tggcgcggcc cgacaatcgt cgtgtgaata gactgatagt
      961 cgttctgttt cggattggaa atgtaatcct tgaatcggcc tggcaccgcg tgccacgtgg
     1021 tgtgcaccac cgaaagtgca tgatagcagt cagccacctc gggcacgacg atgcggaagc
     1081 cgtagatgtc ggaaagttgc tcaagcgaga tttgcttccg ctccatcttg ctccagatgg
     1141 catagggctt cttctcgcgg cccgaggcga tgcccttgac cctggcctgc ttcagcttgg
     1201 tggtcagctc gtcctgaatc tcgtcgatca agccctgatt gcgctcgcgt aggatcttca
     1261 agcgttcgac aaccgtcgca taggcctcgg ggttcagcca acggaaggcg tggtcctcca
     1321 actcatcccg cagccattgc atgcccatgc ggcccgcgag cggcgcatag atgtccatgg
     1381 tctcttcggc gatgatcttt cgcttcgccg gcttcatgtg cttcagcgtc cgcatgttgt
     1441 gcagacggtc ggcgaacttc accagcaaca cccggatgtc gctggagatg gcgagcaaca
     1501 gcttgcggaa attctcggcc tgcgccgcct tcttagtgac gaggtcgagt tttttgatct
     1561 tggtaagacc gtcgaccagc gtgccgatct cagcgccaaa cagcgtgtcg atctccgccc
     1621 gggtcacggt cgtgtcttcg atagtgtcgt gcaaaagggc cgtggcgatc gttgcgtcgt
     1681 cgagacgcat ctcggcgagg atcgccgcca cttcgagagg atgggaaaag tacggagcgc
     1741 cggacgcgcg cttctgattg ccatgcgcct tcatggcgta gacataggcg cggttcagca
     1801 tcgcctcgtt ggcgttgggt tgatagctca gcacccgttc gaccagctcg tattggcggg
     1861 tcatgggttg cgcccgctta tcgctgtgga acgtgacgcg ctttgcggca aagcccggtg
     1921 cggcgaggca atcaaacttc tgccacgccc aaagatccgg tcggcctcag cgggcgcgtg
     1981 gtcccgcatt cgagccatcc gagggcgcct gggtcgccat ccggcgcagc agctcctctt
     2041 cggacatccg gtcgatctgc gtatcggtcg actgatcgtc ctgaccgagt acaggcatcc
     2101 gggtctccga cgagagcatc ggcacggctt ccgcttcggg ctcatccact tcgacatatt
     2161 tctgcatgga gtggatgaaa tcttccttga ggtcgtcggg aaggatcttc ttgtccgcga
     2221 tctcgcggag cgccacgacg gggttcttgt cgttgtcccg ctgcacggcg atctgggcgc
     2281 cggcggagat ggtgcgggcg cggtgggcag ccagcagcac cagctcgaag cggttctgaa
     2341 tcttgtcgat gcaatcttcg acggtgacgc gagccatctg gaagtccttt tgaaagtggc
     2401 cggatttagc ccctatgtat gcagttcgcg gggttctggc aagtcgaacc caggtcatta
     2461 acaagttaac gcttgccgat cacctgccct tcccggctcc acgcgacgct ccgccacacc
     2521 tcctgagcgc ttctgcgggt gacggggtgc cgccagaagg gggccacgtc gagcagcggc
     2581 cgcagcacga aggggcgctt ttcgatccac ggatggggca aaaccagcgg tctgggccct
     2641 gcccgtgcga accgtggatg cttaccccgc caatgcctaa cgaggccctt atagtccagg
     2701 atgtcgatat cgagggtccg tgggccccag gggcttcccc cccggcgccc cgaggcgcgc
     2761 tcgatggcct tcagcacgcg taagagcgct tcgggcggca gcgccgtgtc gatcgcggcg
     2821 gcggcattta cgtaagccgg ttgtcgcgcg cgccccatcg cggctgttcg gtaatacggg
     2881 gaaaccgata tcacagtgac accggcgtcc tgtaattccc gcaaagcccg cgtgatcgtt
     2941 tcggcaggag tcccccaggg tccgggacta ttggaaccca atcctaccaa tacctgcgcc
     3001 ttcgcgacca attgccgagc gtccttgctg tgcctatctt gactagggag ttgttcgtct
     3061 cagttcgttc ccgtccacta atttttttga gcgttaggac ccaagatgaa tgtctatcca
     3121 acagagcgca tcgcgctctt tatcgacggc tccaatcttt acgccgccgc aaaatccctt
     3181 tccttcgaca tcgactacaa gcgtctgctt acgctcttcg gtttaaaggg tcagctggtg
     3241 cgcgcgctct attacaccgc cttgtccgag gaccaagaat actcctcgat ccggccgctg
     3301 atcgattggc tcgactacaa cgggtacacc atggtgacca aaccaaccaa ggagttcgtc
     3361 gatgcggcgg gccggcggaa ggtcaaaggc aacatggata tcgagctcgc ggtcgatgcc
     3421 atggaacttg ccgaacatct cgatcacctc gttatctttt ccggcgatgg tgatttccgt
     3481 tcgctggtcg aagcgctcca atataagggc aagcgcgtga gcgtcgtatc cacgctaacc
     3541 acgaacccgc ccatggttgc cgacgagctg cgccgtcagg ccgatcagtt catcgacctt
     3601 gcgagcctgc agaatgagat tgggcgcgat ccgtcccaac gcacgcagcg cgaacagcgg
     3661 cagcgccccg acggcgagcc gcccaccgat gagttcgagc ccggcgacct catcgagacc
     3721 tgattcggag cttcgcgcga cttgcgaagc ctggcccgcc agccgagact ggcgcggtga
     3781 ctctcattgc gtcccctgcg aaccagcccg aaccggcgtc cgactgcccg ctctgtccgc
     3841 ggctggtgga gttccgtcat gacaaccaga cggcacatcc ggactggttc aatggcgcgg
     3901 tgccatcgtt cggccccgag accgcgcggc tcctcattgt cggcttggcg ccggggctca
     3961 aaggcgcgaa ccggaccagg cgccccttcc ttggcgatca cgccggcgac cttctctatc
     4021 caacgcttct caaggtgggc cttgcccggg gcacgcacga cgtcgagacc cctgaggcgc
     4081 ttcaactgat cgactgcatg atcaccaacg ccgtgcgctg cgtgccgccg cagaacaagc
     4141 ccacatcgca agaagtcaaa acctgccggc cgttcctgat cggccgcatg gaggccctcc
     4201 ccgatttgcg agcgctgctc gcaattgggc gcattgccca tgacagcctc atggatgtct
     4261 tcggcctcaa gaagcgtgat ttccccttct cccacggggc gcggcacaca ttgccaggcg
     4321 ggctcatcct gttcgacacg tttcactgct cccgcctaaa cactaatacc ggccggctga
     4381 cgccagagat gttcgaggcc gtggtcggag ccgctgccgc gagccttgac gaggcaagcc
     4441 tcagcgaggc agccctcaaa gactaattgc tgacatagct caactcgggc atcctcgatg
     4501 tctcctattg ttcgccgagg aggttccgat ggccggaata gaaatctcac cgacgaaggt
     4561 cggcttcgtc attctgaagg cgcgcgagta cggcgcgaag gtcggcgctt gggacgacag
     4621 cgccacgtcc gaccatgatg cggagtcgat cctggaggac ttcgccgacg actccactgt
     4681 cgccgagctg aaagagttca tccacgatct caacgaggac gagcaagtca gcctcgtggc
     4741 gctcgcctgg atcggccgcg gcaccttcag tccagaggaa gtcagcgagg ccatcgatac
     4801 cgcccgcgcc gaacggacca cccgcacgga ggactatctc ctcggaatcc cgttgctctc
     4861 cgattacttg gaagaaggct tggataagct cggctattcc gtcgaggagg ccgaagacgg
     4921 cgccatgggt gactagccca ggcgactagg cggtaactag cgtgtcgcct tctcaagcca
     4981 agcgagcacc tcgtcggcgt gggtcgccgg atccggcacc ggatagaacg gaacctcaat
     5041 cgcgccgtcc ctcaccacaa gtgtgagtcg tttcaggtag gtctgaccgc ctgttgcgaa
     5101 actgggtaat gccagcgccc ccgaaaattc ctctcccgta tcgctgagga gcgggaacgg
     5161 caggcccagg cgcgtgacca gctcctgctg atagccgacc tcctggaggc tcagcccgaa
     5221 cagccgcacg tcaagggcct cgaaccgggg tgagagggcg ccgaagcgct cca
//
LOCUS       DBNHVU010000020        15753 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_156309, whole genome shotgun sequence.
ACCESSION   DBNHVU010000020 DBNHVU010000000
VERSION     DBNHVU010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 15753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15753
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_156309"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="AC16Y7_00745"
     CDS             complement(<1..708)
                     /locus_tag="AC16Y7_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR5707936.1"
                     /translation="MLRSKFVTAVLLGGVVAAVAALGGCGQFSPPYMKPLSARTAMLL
                     GTKGMTEQQPILIRIFKAESELEIWKQKDDGHYYHLKTYPICAYSGGLGPKLKQGDRQ
                     APEGFYLVDKDLMNPKSKYYLAFNMGYPNAYDRSFGRTGANLMVHGDCTSSGCYAMTD
                     AVMEEIYVLAREAFAGGQEKFQVHAFPFRMTKANLAAQKEHDWYTFWKNLKEGYDFFE
                     TTRLAPALAVCGKRYVVN"
     gene            complement(885..1838)
                     /locus_tag="AC16Y7_00750"
     CDS             complement(885..1838)
                     /locus_tag="AC16Y7_00750"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HIR5707937.1"
                     /translation="MRTYLDFEKPIAELESKVAELKALVADDESVPMTEELARLESKA
                     HKALVETYAELTPWQKTQVARHPERPHFKHYIEGLVADFTPLAGDRYFAEDEAIIGGI
                     GRLSGLSVMVIGHEKGSDTEGRLKHNFGMARPEGYRKAVRLMEMADRFSMPVVSLVDT
                     AGAYPGIGAEERGQAEAIARCTDCCLRLGVPIVSLIVGEGGSGGAVAIATANRIYMLE
                     HSIYTVASPEAAASILWRDSARAQDAATNMKITAQDLLRFGIVDGIIPEPVGGAHRDT
                     EGVIKAAGDVIVKAITEFDGRSADEIRKDRREKFLAMGRAG"
     gene            complement(1974..2942)
                     /locus_tag="AC16Y7_00755"
     CDS             complement(1974..2942)
                     /locus_tag="AC16Y7_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIR5707938.1"
                     /translation="MAPAKGSSRRADSSELALFLDMLMAERGAASHTIDAYARDISDF
                     LAFLAAKKVGAKKASADHVRAYLESLSDRGLASTSRARKLSAIRQFFRFLLGEGLRVD
                     DPTSAVDSPKLGRPLPKVLSLAEVETLIDTAKQSCAISTQGAPWTRALRLYALLETLY
                     ASGMRVSELIALPRNVLAADDRVLTIKGKGGRERLVPLNEAAREALAAHRAALGEAGL
                     DASLWLFPTGDGGQHITRQRFGQELKALALKAGLDPARVSPHVLRHAFASHLLERGAD
                     LRTVQQLLGHADISTTQIYTHVIEERLRRLVEQHHPLAQAHLTKRR"
     gene            complement(2987..3205)
                     /locus_tag="AC16Y7_00760"
     CDS             complement(2987..3205)
                     /locus_tag="AC16Y7_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707939.1"
                     /translation="MPTLFRFLAVLGTIAGVVAGSLYVLAEYFQPEPKEISKALRNVK
                     ATQRAADVSGAPTKIEPSIVLPPSHRAQ"
     gene            3365..3979
                     /locus_tag="AC16Y7_00765"
     CDS             3365..3979
                     /locus_tag="AC16Y7_00765"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR5707940.1"
                     /translation="MAEHNKDDRTAQLAEIRQLLGARSVVLIGLMGAGKTAVGRRLAN
                     WLDLPFTDADSEIEAAADQTIKEIFATHGEAYFREGEQKVITRLLENGPQVLATGGGA
                     YMNPKTRAAVKANGISVWLRADLRVLLKRVGRRDHRPLLANGNPETVMKKLMAERDPI
                     YAEADIIVESREVPHEVIVGAVVDALGAELGCKAKPRARRRRKA"
     gene            3983..5128
                     /gene="aroB"
                     /locus_tag="AC16Y7_00770"
     CDS             3983..5128
                     /gene="aroB"
                     /locus_tag="AC16Y7_00770"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015917340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR5707941.1"
                     /translation="MDDTATSTNLGETTIDVALAERGYHVVIGEDLLASAAGRLKALL
                     PGARFAVVSDSNVAPLHMGPLKESLEQDGLFLGSAVVAPGEASKSFPVLAKLCETLLD
                     LGVERGDCIIALGGGVVGDLAGFAASILRRGVRVVQVPTSLLAQVDSAIGGKTGIDTS
                     QGKNLIGTFHQPNLVLADVDVLSTLSEREFRAGYAEVAKYGLLGDAPFFVWLEQNWQG
                     VFADETGKRRHAVETSAHAKVDIVVADEREESGTRALLNLGHTFGHALEAFAGYSDRL
                     LHGEAISIGMRLAFTYSVERDLCTPDDAARAEAHFKAVGLPTDIATIPGDKPTPGELL
                     RLMAQDKKVAGGKLVLVLVKGIGQAFIEHDVSMEQMTDFLKRECARR"
     gene            5125..6426
                     /locus_tag="AC16Y7_00775"
     CDS             5125..6426
                     /locus_tag="AC16Y7_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HIR5707942.1"
                     /translation="MTLALGLTLGAIVGLLVVSAFFSGSETALTAASRARMHALEEDD
                     NARAKLVNRLLSSRERLIGALLLGNNLVNIAASALATSLFLSVFGEAGVVYATIVMTV
                     LVVVFAEVLPKTYAILNADRMALAVSPILKWLVAVLAPVTAALQFLVRHTLRLFGSSI
                     ADDADVLSAHEEIRGAIELHHKEGGVVKLDRDMLGGVLDLRELDVSDIMVHRTNMDAI
                     DAELPPGEIVDAALKSPHTRLPLWRGEREEIVGILHARTLLQALRDSEGDVSKIDVMS
                     LATSPWFVPDTTTLKAQLSAFLKEKAHFAIVVDEYGEVMGLVTLEDIIEEIVGDITDE
                     TDIGASLAKPQADGSLIVDGLVPIRDVNRLMEWDLPDEEATTVAGLVLHEAQTIPNPG
                     QAFTFHDFRFEILRRHRNRITSLRVTPLDDARPADAETSEA"
     gene            complement(6430..6699)
                     /locus_tag="AC16Y7_00780"
     CDS             complement(6430..6699)
                     /locus_tag="AC16Y7_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HIR5707943.1"
                     /translation="MSIEQSIREKLTEAFQPDALDVVNESHLHAGHAGSPGTGESHFA
                     VKVVSAAFAGKSRLERHRMVNDVLAEEMSGNIHALALATLAPEEA"
     gene            6821..7444
                     /locus_tag="AC16Y7_00785"
     CDS             6821..7444
                     /locus_tag="AC16Y7_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIR5707944.1"
                     /translation="MKLNSKFFDGLRVKPDKDRRLRDTTPKCDWEGCSEPGLYPAPKG
                     RGLEGQYHRYCLDHVRAYNKGYNYFSGMPDDDVVEHQKDAATGNRPTWFVGVNSTSRN
                     RTKRGKPGGYSHRFDAEDPFRLFGENAPGGKPPGEAPSRPLKRLERKALRQLNLEDNA
                     SKPEIKTRFKELVKRLHPDHNDGDRSSEDKLREVIQAYNYLRQAGLA"
     gene            7531..8520
                     /gene="cobS"
                     /locus_tag="AC16Y7_00790"
     CDS             7531..8520
                     /gene="cobS"
                     /locus_tag="AC16Y7_00790"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HIR5707945.1"
                     /translation="MTLEAAQNGAGLPDMTVSVRQVFGIDSDLEVPAYSQPDEHVPDV
                     DPDYLFNRDVTLAILAGFKHNRRVMIQGYHGTGKSTHIEQIAARLNWPCLRINLDSHV
                     SRIDLVGKDAIVIKDGKQVTEFREGILPYALQTNTALCFDEYDAGRPDVMFVIQRVLE
                     VSGKLTLLDQSKVIRPHPAFRLFATTNTIGLGDTSGLYHGTQQINQGQMDRWSIVATL
                     NYLPHDDEVDIVVAKAKTWNTEEGRKTVSNMVRVAELTRNAFMNGDLSTVMSPRTVLT
                     WAENAEIFGDVGEAFALSFLNKCDELERGLIAEFYQRCFGEDLPQSTANVALS"
     gene            8525..10426
                     /gene="cobT"
                     /locus_tag="AC16Y7_00795"
     CDS             8525..10426
                     /gene="cobT"
                     /locus_tag="AC16Y7_00795"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HIR5707946.1"
                     /translation="MTLNKRKEAPLDPFKRAVTQTTRSIAADGSVEVVFSTEPPGLAG
                     KTARLPEPSRVPTMNEIAVIRGHADAAALAVACHDEKLHQALAPSGGDARAVFEAIEQ
                     ARVEAIGAHRMEGVAANLNARMEQRYARSRITEDTERSEAPLPDALALLVRERLTGIA
                     PPDRARALVNLWRPWIEERANTLLDRMPDTVEDQEAFGRLARDVLAALNLADQLDQGE
                     QDEESTTDGESDPNASDEEDESAEGQDQSELAQDANAEPDDTSEETLEDQEDGPTDDL
                     DREADAADLPRRSDPWQPNVSVLDDPEAFGYKVFTRAFDEIIGAEELCDIDELDRLRA
                     FLDKQLVALHGAVARLANRLQRRLLAQQNRGWDFDLEEGVLDAARLPRVIIDPMHPLT
                     FKQERDTDFRDTVVTLLLDNSGSMRGRPIMVAACCADILARTLERCGVKVEILGFTTR
                     AWKGGHAREAWLNAMKPALPGRLNDLRHIVYKSADAPWRRARRNLGLMMREGLLKENI
                     DGEALAWAHQRLLARPEQRRILMMISDGAPVDDSTLSVNSGAYLERHLRQVINEIETR
                     SPVELLAIGIGHDVTRYYRRAVTVTDAEELAGVMTEKLAELFDEDLAWRQLHRAVPTA
                     TRQKRRKLH"
     gene            10438..11475
                     /locus_tag="AC16Y7_00800"
     CDS             10438..11475
                     /locus_tag="AC16Y7_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HIR5707947.1"
                     /translation="MIRRPAPALASIVLAGAVLLAVGADLASKPATVLEGPIKPRVTA
                     IAVAFDRDNPKQKTFGKLTYRGGFNLFANSRHFGGYSGLAIDASGTTILAVSDGGTWM
                     RASLDYNGRLLQGVHDMTLGPILGPNGKPLRKGKDRDAEGLALASGDTRNGEAYVSFE
                     RVHRIARYPFSPKRFGPPSRTVKLPKAARQMKTNQGLEAVALIRAGRLTGTIVAFSER
                     LKDSRGNLKGWLIGGPSPGTITVRRRGGFDITDAAGLPDGGLILLERRFRYSEGVQMR
                     IRRIKAADLRPGKLIAGEILLEATDRLNIDNMEAVAVHRSRGGETILTLMSDDNFNPL
                     QRSLIMQFALP"
     gene            complement(11489..11788)
                     /gene="rpmB"
                     /locus_tag="AC16Y7_00805"
     CDS             complement(11489..11788)
                     /gene="rpmB"
                     /locus_tag="AC16Y7_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR5707948.1"
                     /translation="MARRCELSGKAVLTGNNVSHAKRRTRRRFLPNLCNVTLQSDVLK
                     KRVRMTVSAHALRSVEHRGGLDAYLLKAGDDELSLKARRLKREIAKASQAPAAAS"
     gene            12016..12882
                     /locus_tag="AC16Y7_00810"
     CDS             12016..12882
                     /locus_tag="AC16Y7_00810"
                     /inference="COORDINATES: protein motif:HMM:NF022750.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HIR5707949.1"
                     /translation="MRSARHCLSLLAVAGLTLGLAPTLDDERALAEAPRTSAARISAV
                     YKVNLGRFNLGNFRVTTTVQEGGYQLRGEGTFSVLEGLIFKWQGITSSRGRVTDEGPA
                     PSSYAFNYSDGGKNSERLRITFGNGAVQQVSIVPKRRIPGLIPVRREQLVGVIDPMSG
                     AFLTARSPNPNGDLSVCNQMLPVFDGRSRFDLVLKPKKRVMVQNRSRSSYSGPAAVCR
                     VKFVPISGYRPDDAGIQHMKQSNEIEVWLMPVRGTDMYVPYRIVLPTPVGYGTAVITS
                     IKVSGARRASVD"
     gene            13352..>15753
                     /locus_tag="AC16Y7_00815"
     CDS             13352..>15753
                     /locus_tag="AC16Y7_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR5707950.1"
                     /translation="MTSSLSTQSTSLAKGRGVTAVLGPTNTGKTHLAIERMLAHEVGV
                     IGLPLRLLAREVYDKVVARAGAEQVALITGEEKIKPPAARFYVCTVEAMPRELEADFV
                     AVDEIQLAADGERGHVFTNRMFHARGLSETLLLGSATMRDAIRELLPGANLVSRPRLS
                     KLSYAGDKKITRLPRRSAIVAFSANDVYAIAELVRRQRGGAAVVLGALSPRTRNAQVA
                     LYQNGDVDFLVATDAIGMGLNLDLDHVAFAGLRKFDGHSHRNLTPSEVGQIAGRAGRH
                     MNDGTFGVTGKVEPLESELVERLEDHAFDQVGVLQWRSRSLDMASLEALKESLREAPN
                     HPRLTRSRMVDDVIALENVTQEADICAMAAAPAAVGLLWEMCQIPDYRKVSPNSHADL
                     VATLYRFVMSDEGKIPEDWFANQVAQSDRTDGDIDTLSNRLAQIRTWTFVSHRPQWLH
                     DPEHWQRRTRAIEDTLSDALHERLAQRFVDRRTSVLMRRLRDKEEMMAEISDDGQILV
                     ENHYVGRLDGFCFTPDASSDGIDGKAARHAAVQVLGTELANRTAALVAAPDETIALDP
                     HGRIVWQGSGIARLERGETALKPRIHLFVDEHLSPPDRERIAARLDAWLAAHLDAKLK
                     PLIALSEAQDLTGLARGLAFRLTETLGVLRRDTAAEEIRSLDQADRGQLRKYGVRFGA
                     FNLYFPALLKPAAAELLLLLWALHDGDAHGLNVDGLPERPQQGLTSVAADNAIPEPYW
                     RAAGFHVAGSRAVRIDMLERLSDLIRARITWRPTENAEDAPPTGATGDGGFRAVPDLM
                     SMV"
BASE COUNT         2949 a         5135 c         4938 g         2731 t
ORIGIN      
        1 gttgaccacg tagcgcttgc cgcatacggc gagcgccggc gccagccgcg tggtttcgaa
       61 gaagtcgtag ccttccttca ggttcttcca gaacgtgtac caatcgtgct ctttctgcgc
      121 cgccaaattg gccttggtca tgcggaacgg aaaggcgtgc acttggaact tctcttgccc
      181 gccggcgaac gcctcgcgcg cgaggacata gatctcctcc atcaccgcgt cggtcatggc
      241 gtagcagccc gaggaggtgc aatcgccatg caccatcaga ttggcccccg tgcggccgaa
      301 cgagcggtcg taggcgttgg ggtagcccat gttgaaggcg aggtagtatt tgctcttcgg
      361 attcatcagg tccttgtcga ccagatagaa cccttcaggc gcctgccggt cgccctgctt
      421 cagcttgggc ccgagcccgc ccgaataggc acagatcgga taggtcttca ggtggtagta
      481 gtgcccgtcg tccttttgct tccagatctc cagctctgac tcggccttga agatgcggat
      541 gagaatgggc tgctgctcgg tcataccctt cgtgccaagg agcatggcgg tccgcgccga
      601 gagcggcttc atgtaaggcg gcgagaactg gccacagcct ccgagcgcgg ccaccgcggc
      661 gaccacgccc ccaagaagta cggccgtgac aaacttcgaa cgaagcatcg cagggcccct
      721 cgcgcacccc gaccaagatc aagttaggtt ggttaacgaa acgttagcgc gagcacccta
      781 cacccccact acgccccgga tttcctcgat tttggccata ctatacgctt gccgatatcg
      841 agttggcctc aaaaaaaagg cgtattttgg ggcgccggag gcgcttagcc ggcccgcccc
      901 atggcgagaa acttctcgcg ccggtctttg cgaatttcgt ccgccgagcg gccgtcgaat
      961 tcggtgatgg ccttcacgat gacgtcgccg gccgccttga tcacaccctc cgtatcgcga
     1021 tgggcgccgc ccacgggctc gggaatgatg ccgtcgacaa tgccgaaccg cagcaggtcc
     1081 tgggcggtga tcttcatgtt tgtcgccgcg tcctgggcac gggccgaatc ccgccacagg
     1141 atcgacgcgg ccgcttccgg cgaggcgacc gtatagatgg agtgctccag catgtagatg
     1201 cggtttgccg tggcgatggc caccgcgccg ccagacccgc cctcaccgac gatgagggaa
     1261 acgatgggca cccccagccg caggcagcaa tcggtgcagc gggcgatcgc ctccgcctgc
     1321 ccgcgctcct ccgcgccaat gccgggatag gcgccggccg tatcgaccag gctcacgacg
     1381 ggcatcgaga agcgatcggc catctccatc aggcgcaccg ccttgcggta accttccggg
     1441 cgcgccatgc cgaaattgtg cttgagccgc ccttccgtat cggagccctt ttcatggccg
     1501 atgaccatga ccgagaggcc cgagaggcgc ccgatcccgc caatgatggc ctcgtcctcg
     1561 gcaaaatagc ggtcgccggc cagcggcgtg aaatcggcca ccagcccttc gatatagtgc
     1621 ttgaaatgcg gacgctcggg gtggcgggcc acctgcgtct tctgccacgg cgtcagctcg
     1681 gcgtaggtct ccaccagcgc cttgtgagcc ttgctctcca gcctcgccaa ttcctcggtc
     1741 atggggaccg attcatcgtc ggcgacaagc gccttcaact cggcaacctt gctttcgagc
     1801 tccgcaatcg gcttttcaaa atcgagatag gtacgcatgc gcctcggcta ttccccgccg
     1861 gaaaccccag ttttcctcgg cgggcactct tataacaaaa cggaaggcca cgggaatccg
     1921 gatgtaagtg gccttgtgag cgggacctgt caaccggcgc ccgtctttgg agcctagcgg
     1981 cgcttggtta agtgagcctg agcgaggggg tgatgctgct ccaccagccg gcgcaggcgc
     2041 tcttcgatca catgggtgta aatctgcgtc gtggagatgt ccgcgtgtcc gagcagctgc
     2101 tggacggtcc gcagatcggc gccccgctcc agaagatggc tggcgaaggc atggcgcagc
     2161 acatggggag agacccgcgc cggatcgagc ccggccttga gcgccagcgc cttaagctcc
     2221 tggccgaagc gctgacgcgt gatgtgctga ccgccatcgc cagtgggaaa gagccacagc
     2281 gatgcgtcga gccccgcctc gcccaaggcc gcccggtgcg cggcgagtgc ctcccgggcc
     2341 gcctcgttga gcggcaccag ccgctcccgc ccgcctttgc ccttgatcgt caggaccctg
     2401 tcgtcggcgg cgagcacatt gcggggaagc gcaatcagct ccgacacccg catgcccgag
     2461 gcatagagcg tctcgaggag cgcatagagc cgcaacgcgc gcgtccacgg cgcaccttgc
     2521 gtagaaatgg cgcaggactg cttggccgta tcgatcagcg tctccacctc ggcgagcgac
     2581 aggaccttcg gcaaggggcg gccgagcttc gggctatcga cggccgaggt cggatcgtcg
     2641 acacgcaggc cttcgcctag gagaaagcgg aagaactggc ggatcgcgga aagcttgcgc
     2701 gcgcgcgagg tggaggcgag ccctctgtcc gaaagacttt cgagataggc gcgcacgtga
     2761 tcggcagatg cctttttggc gccgaccttc ttcgctgcca ggaaagcgag gaagtcggaa
     2821 atgtctcgcg cataggcgtc gatcgtatga gacgcagcgc cgcgctcggc catgagcatg
     2881 tcgaggaaca gcgccagctc ggagctgtcg gcacgccggg aggacccttt tgcgggggcc
     2941 atggctctag gcccttccag gcgcccaaat ggcaccgaaa aacggactat tgcgcgcggt
     3001 ggctgggcgg aagaacgatt gagggttcga tcttggtcgg ggcacccgat acgtcggccg
     3061 cgcgctgggt cgccttgacg ttgcgcaacg ctttggagat ttccttgggc tctggctgaa
     3121 agtattcggc gagcacatag aggcttccag cgacgacacc ggcgatcgtc cctaagactg
     3181 caaggaaacg aaacagggtt ggcattgagg cgctcgtatt cctaactatc tgtgggttcc
     3241 gaccaggcgg tattatgact caaaacgccc gagcgcctta acccttcaat cgcatcatgg
     3301 acgattcgac acggccctgc cagcatggga cattgggccg cccttcgcac atatgacccc
     3361 acgcttggct gaacataata aagacgaccg cacggcgcag ttagctgaaa tccgccaatt
     3421 gctgggcgcg cgctcggtcg tcctgatcgg cttgatgggc gccgggaaga cggctgtcgg
     3481 ccggcgccta gccaattggc tcgatttgcc cttcaccgac gccgacagcg agatcgaggc
     3541 agcggcggac cagaccatca aggagatctt cgccacgcac ggtgaggcct atttccgcga
     3601 gggagagcag aaggtaatca cccggctcct ggagaatggc ccgcaagtgc ttgccacggg
     3661 cggcggcgcc tatatgaacc ccaagacgcg cgccgccgtt aaggcaaacg gtatctcggt
     3721 gtggctcagg gcggacttgc gggttttgtt gaagcgcgtc gggcgccgcg atcaccggcc
     3781 gttgctggct aacggcaacc ccgaaaccgt gatgaagaaa ttgatggctg aacgcgatcc
     3841 catttacgcc gaagcggaca tcatcgtgga gagccgcgaa gtgccccacg aagtgatcgt
     3901 cggcgccgtg gtcgatgcgc tgggtgctga actcgggtgc aaggccaagc cgcgggcacg
     3961 ccggcggcgg aaggcttaag caatggacga caccgcgaca tctaccaatt tgggtgagac
     4021 gaccatcgac gtcgcgctgg ccgagcgcgg ctatcatgtc gtcatcggcg aagatttgct
     4081 ggcgtccgcg gccgggcggc tcaaggcgct tctgcccggc gcacgcttcg ccgtggtcag
     4141 cgactccaac gtggcgcccc ttcatatggg cccactcaag gagagtctcg aacaggacgg
     4201 actgtttctt ggctccgcgg tcgttgcgcc tggcgaggcc agcaagagct ttccggttct
     4261 tgccaagctg tgcgagacgc tgctggacct tggcgtcgaa cgcggcgatt gcatcatcgc
     4321 gctcggcggc ggcgtggtcg gcgacttggc agggttcgct gcctcgattt tgaggcgcgg
     4381 cgtgcgggtc gtgcaggtac cgacatcgct cctggcgcaa gtggattccg caatcggcgg
     4441 caagaccggc atcgacacct cgcaaggcaa gaacctgatc ggcacgtttc atcaaccgaa
     4501 cctggtgctc gccgacgtgg atgtgctgtc gacattgagc gagcgggagt ttcgtgccgg
     4561 ctatgccgaa gtggccaaat acgggctgtt gggcgatgcg cccttcttcg tgtggctgga
     4621 gcaaaactgg caaggcgtgt tcgccgacga gaccggcaag cgccgccacg ccgtggagac
     4681 cagcgcgcac gccaaggtgg acatcgtcgt ggccgacgag cgcgaggaaa gcggcacgcg
     4741 ggcgctgctc aatctcggcc acacctttgg tcacgcgctg gaggccttcg ccggctattc
     4801 cgatcggctg ctgcacgggg aggccatttc catcggcatg cgcctcgcct tcacctattc
     4861 ggtggagcgg gacctctgca cgccagacga cgccgcgcgc gccgaagcac atttcaaggc
     4921 cgttggcctt cccaccgaca tcgcgaccat ccccggcgac aagccgacgc cgggcgaact
     4981 gttgcgtctg atggcccaag ataagaaagt cgcaggcggc aagctcgtgc tggtactcgt
     5041 caagggtatc ggccaggcct tcatcgagca cgacgtctcc atggagcaga tgaccgactt
     5101 cttgaagcgg gagtgcgcgc gccgatgacc ttagcgctag gtctcacgct cggcgccatc
     5161 gttggcctcc tcgtcgtctc cgccttcttc tccggttcgg agacagcatt gacggcggca
     5221 agccgcgccc gcatgcacgc gctcgaagag gacgacaatg cgcgcgcaaa gctcgtaaac
     5281 cggcttctgt cctcgcgcga gcgcctaatc ggcgcactgc tcctcggcaa caatctcgtc
     5341 aacatcgcag catccgcgtt ggcgaccagc ttgttcttgt cggtgttcgg cgaagcgggc
     5401 gtcgtctatg ccacgattgt catgacggtt ctcgtcgtcg tgttcgccga ggtgctgccc
     5461 aagacctacg ccatcctcaa cgccgaccgc atggcgctgg ccgtatcgcc gatcctcaaa
     5521 tggctcgtcg ccgtactcgc acccgtgacc gccgcgctgc aatttctcgt gcggcacacc
     5581 ttgcgcttgt tcggctcgtc catcgccgat gacgccgacg tattgtccgc ccatgaggag
     5641 attcgcggcg ccatcgagct gcatcacaag gaaggcggcg tggtgaagct cgaccgcgac
     5701 atgctgggcg gtgtgctcga cctgcgcgag ctcgacgtct ccgacattat ggtgcaccgg
     5761 accaacatgg acgcgatcga cgccgaactg ccgccgggcg agatcgtcga cgcggccctc
     5821 aaaagcccgc acacgcggct accgctatgg cgcggcgaac gcgaggagat cgtcggcatt
     5881 ttgcacgcgc gcacgctgtt gcaggccttg cgggactcag aaggcgacgt ttcgaagatc
     5941 gacgtcatgt cgctggcaac ctcgccatgg ttcgtgcccg acacgacaac gctgaaagcg
     6001 cagctcagcg ccttcctcaa ggagaaggcg cactttgcca tcgtcgtgga cgagtacggc
     6061 gaggtgatgg ggctggtgac gctggaggat attatcgagg agatcgtcgg cgacatcacc
     6121 gacgagacgg atatcggcgc ttccctcgcc aagccgcagg ccgacgggtc gctgatcgtc
     6181 gacggcctcg tgccgatccg cgacgtgaac cggctaatgg agtgggacct gccggacgaa
     6241 gaggcgacca ccgtggccgg cctcgtcctt catgaggcgc agaccattcc caatccgggt
     6301 caggccttca ccttccatga tttccgcttc gagatcttgc ggcggcatag gaaccgcatc
     6361 acttcgttgc gcgtgacgcc tttggacgat gcgcgcccgg ccgacgccga gaccagtgaa
     6421 gcctaggctc taagcctcct cgggggcgag cgttgcgagc gccagcgcat ggatattgcc
     6481 tgacatctcc tcggccagca cgtcattgac catgcggtga cgctccagcc gggacttgcc
     6541 ggcgaaggcg gccgacacca ccttgacggc gaaatggctc tcgcccgtgc ccggcgagcc
     6601 ggcatggccc gcgtggagat gggactcgtt gacgacatcg agcgcgtccg gctggaaggc
     6661 ttcggtcaac ttttccctga tcgactgctc aatgctcatt ggcttgatgg tccttggctg
     6721 catgtggcgg tgcgtggcat tcaaccatac agattatcgc gtttgtcatc acggagccat
     6781 gcttgttgta accaatgctc gttcccataa tgccgattcg atgaagctca actcaaaatt
     6841 tttcgacggc ctgcgcgtca agcccgacaa ggaccgtcgg ctgcgcgaca ccacccccaa
     6901 atgcgactgg gaaggctgta gcgagccagg cctctatccg gcgccgaagg gccgtgggct
     6961 cgagggtcag tatcaccgct actgcctcga ccacgtccgc gcgtacaaca agggctacaa
     7021 ctatttttcc ggaatgcctg acgacgacgt cgtcgagcac cagaaggacg ccgcgaccgg
     7081 caaccgcccg acctggttcg tcggcgtcaa ttccacctcc cgtaaccgca caaaacgcgg
     7141 caagcccggg ggctacagcc accggttcga cgccgaggac ccgttccgcc tgtttggcga
     7201 gaatgcgccg ggcggcaagc cccccgggga ggccccgagc cgccctctga agcgcctgga
     7261 acgcaaggcc ctccgccagc tcaatctgga ggacaacgcc tccaagcctg agatcaagac
     7321 ccgcttcaag gaattggtta agcgcctcca ccccgaccac aatgacggcg accgctcctc
     7381 ggaggacaag ttgcgcgaag tgatccaagc ctataattat ttaaggcagg cggggctcgc
     7441 ataagtcgct gggccagccc ccggcaaatc caggctctgc cctctggcga gcctctactg
     7501 aaacgcgaca cacaggctca tcttaagaca atgacacttg aagctgctca gaacggtgcc
     7561 ggtctgccgg acatgaccgt ttccgtccgc caggtcttcg gcatcgatag cgatctcgag
     7621 gttcccgcct attcccagcc ggacgagcac gtcccggacg tggaccccga ctatctgttc
     7681 aacagggacg tgacgctggc catcctcgcg gggttcaaac acaaccgccg cgtgatgatt
     7741 cagggctatc acggcaccgg caagtcgacc catatcgagc agatcgccgc gcggctgaat
     7801 tggccgtgcc tccgcatcaa tctggacagc cacgtgagcc gcattgatct cgtcggcaag
     7861 gacgccatcg tcatcaagga cggcaagcag gtcacggagt tccgcgaggg tatcctacct
     7921 tacgcgcttc agaccaacac cgcgctgtgc ttcgacgaat acgacgccgg gcgtcctgac
     7981 gtgatgttcg tcattcagcg cgtgctggag gtctccggca aactgacctt gctcgaccag
     8041 tcgaaggtca tacggccgca tccggccttc cgcctcttcg ccaccacgaa cacgatcggt
     8101 ctcggcgata cgagcgggct ctatcacggc acgcaacaga tcaaccaagg tcaaatggac
     8161 cgctggtcga tcgttgcaac gctcaactat ctgccacacg acgacgaggt cgacatagtc
     8221 gtggccaaag ccaagacgtg gaacacagag gaaggccgca agaccgtgtc gaacatggtt
     8281 cgagtcgccg agttgacgcg caacgccttt atgaatggcg acctctccac ggtgatgagc
     8341 ccgcgcacgg ttctgacctg ggcggagaac gcagagattt tcggtgatgt cggcgaagcc
     8401 ttcgcgctgt cgttcctcaa caaatgtgac gaactggagc gcggcctgat cgccgagttc
     8461 tatcagcgct gtttcggcga ggacttgccg cagtcgacgg ccaacgtagc cttgagctaa
     8521 tcccatgacc ctcaacaagc ggaaagaggc gcccctcgat ccgttcaaac gcgcggtaac
     8581 acagaccacg cgctcgatcg ccgccgatgg cagcgtggag gtggtgttct cgacggagcc
     8641 gccgggtctt gccggcaaga ccgcgcgcct gccggagccg tcgcgcgttc cgacgatgaa
     8701 cgagattgcc gtcatccgcg gccatgcgga cgccgcggcg ctcgccgtcg cctgtcacga
     8761 cgaaaaactg caccaggcgc tcgcgccctc cggcggcgat gcccgcgcgg tgttcgaggc
     8821 gatcgaacaa gcccgcgtgg aagccatcgg cgcacatcgc atggaaggcg ttgccgccaa
     8881 tctgaacgcg cgcatggagc agcgctacgc aagaagccgc atcaccgagg acaccgagcg
     8941 tagcgaggcg cccctgcccg acgcgcttgc ccttttggtg cgtgagcggt tgacgggcat
     9001 cgccccgccg gaccgtgccc gcgcgctcgt caatctttgg cgtccctgga tcgaggagcg
     9061 ggccaacacc ctactcgacc gcatgccgga tacggtggag gaccaggagg cctttggccg
     9121 cctcgcccgc gacgtgcttg cagcgctgaa cctcgccgat cagctcgatc agggcgagca
     9181 ggacgaggag tcgaccacgg acggtgagtc cgatcccaat gcctcggatg aagaagacga
     9241 aagtgccgag ggacaggacc agtccgagct ggcgcaggat gccaacgccg agccggacga
     9301 cacatccgag gaaacgctgg aggaccaaga ggacggcccc actgacgacc tcgaccgcga
     9361 ggccgacgcc gccgatctgc cgcgtcgctc cgatccttgg caaccgaacg tctctgtgct
     9421 cgatgacccg gaggccttcg gctacaaggt cttcacccgc gccttcgacg agatcatcgg
     9481 cgccgaagag ctgtgcgaca tcgacgagct cgaccgtctg cgcgcattcc tcgacaaaca
     9541 actggtcgcg cttcacggcg ccgtggcccg cctcgccaac cggctgcagc gccggctgct
     9601 ggcgcagcag aaccgcggct gggacttcga tcttgaggag ggcgtgctcg atgcggcccg
     9661 tctgccgcgg gtcatcatcg accccatgca tccgctgacc ttcaagcagg aacgcgacac
     9721 ggacttccgc gataccgtgg tgacgctgct cctggacaat tcgggctcca tgcgcggacg
     9781 ccccatcatg gtcgccgcct gctgcgccga tatcctggcg cggaccctgg agcgttgtgg
     9841 cgtgaaggtc gagatcctcg gcttcaccac gcgcgcctgg aagggcgggc acgcacgcga
     9901 ggcgtggctc aacgccatga agccggcgct tcccggacgg ctcaacgacc tgcgccacat
     9961 cgtctacaag tcggcggatg cgccgtggcg ccgggcacgc cgcaatctcg ggctcatgat
    10021 gcgcgagggg ctgctcaaag agaacatcga cggcgaagcg ctggcctggg cgcaccagcg
    10081 gctattggcg cgtcccgaac agcggcgcat tctgatgatg atctccgacg gcgcgcccgt
    10141 cgacgattcc acgctctcgg tcaactcagg cgcctatctg gagcggcatc tgcgtcaggt
    10201 gatcaacgag atcgagaccc gatcgcccgt cgaattgctc gccatcggca tcggccatga
    10261 cgtgacgcgc tattaccggc gcgccgtcac cgtaaccgac gccgaagaac ttgccggtgt
    10321 catgacggag aagctggcgg agctgttcga cgaggacctc gcctggcggc agctgcatcg
    10381 cgccgtcccg acggccacgc ggcaaaagcg ccgcaagctc cactaggttt cggccccatg
    10441 atccgaagac ccgcaccggc gctcgcgtcc atcgtgctcg cgggcgcggt gctgctcgcc
    10501 gtgggcgcag accttgccag caaacccgcg actgtcctcg aagggccgat caagccgcgc
    10561 gtcaccgcca ttgccgtcgc gttcgaccgc gacaacccca agcaaaagac cttcggcaag
    10621 ctgacctatc ggggtgggtt caatctgttc gccaattcgc ggcacttcgg cggctattca
    10681 ggcctggcca tcgatgcgtc ggggaccacg atcctcgccg tctccgacgg tggcacctgg
    10741 atgcgtgcct cgctcgatta taacggccgc ttgctgcagg gcgtgcacga catgacgctc
    10801 ggacccatcc tcggccctaa tggaaaaccg ctgcgcaagg gcaaggaccg cgacgccgag
    10861 gggctggcgc tggcctccgg cgatacgcgc aacggcgagg cctatgtctc cttcgagcgc
    10921 gtgcaccgta tcgcgcgcta ccccttcagc ccgaaacgct tcgggccgcc gagcaggact
    10981 gtgaaactgc caaaggccgc gcgccaaatg aagaccaatc aaggcctcga ggcggtcgcg
    11041 ctgatccggg ccggacggct gacaggcacc atcgttgcct tctccgagcg gctgaaggat
    11101 tcgcgcggca acctcaaggg ctggctgatc ggcggcccga gcccgggcac cattacggtc
    11161 cgccggcgcg gcgggttcga catcaccgat gccgcagggt taccggacgg aggcttgatc
    11221 ctgctggagc gccgcttccg ctacagcgaa ggcgtgcaga tgcgcatccg ccgcatcaag
    11281 gcagcggact tgcggcctgg aaaattgatc gccggagaga tcctgctgga ggcgacggac
    11341 aggctcaata tcgacaacat ggaggcggtc gcggtccatc gctcgcgcgg cggcgagacc
    11401 atcctgacat tgatgtctga tgacaatttc aacccgctac agcggtcgct gatcatgcag
    11461 ttcgctctgc cgtgagcgaa ggcaggcgct aagacgccgc ggccggcgcc tggctggctt
    11521 tggcgatctc acgcttgagg cgccgcgcct tcagcgacaa ttcgtcatcg cccgccttca
    11581 gcaggtaggc atcaagcccc ccgcgatgct cgaccgagcg cagcgcatgg gcggagacgg
    11641 tcatccggac gcgcttcttg agcacgtcgc tctgcagcgt cacattgcac agattcggca
    11701 gaaaacggcg ccttgtgcgg cgcttggcat ggctcacatt gttgccggtg agcacggctt
    11761 tgccggatag ctcgcagcga cgcgccatgg tccttgtcct tctccgaata tcttgtggaa
    11821 ccctgaggcg gcctgtgaca ttcacaagca caaaggcacc agggaggcga ggtatacggg
    11881 gctcgcccgg ttcggtcaag acaccggctc gatttcgatc gaagccagcc gaattgacga
    11941 atccccaaaa tacctggaca ttcgaagggg tctggggcgt cgaatgaagg gacgtctagc
    12001 cagaggtcgg gagccatgcg gtcagcacga cattgcttga gcctgttggc ggtggccggc
    12061 ctcactctgg ggctcgcgcc caccctggat gacgaacgcg cgctcgccga ggccccgcgc
    12121 acgtcggcgg cccgcatctc cgccgtctac aaggtcaatc tcggcaggtt caatctgggc
    12181 aatttccgcg tcaccaccac ggtgcaggaa ggcggatatc agctgcgcgg cgaaggcacc
    12241 ttcagcgtcc tggagggcct gatcttcaaa tggcaaggga tcacgtcaag ccgcgggcgc
    12301 gtcaccgacg aaggcccggc gccctcttct tatgccttca actattccga tggcggcaag
    12361 aacagcgagc gcttgcgcat aacgttcggc aacggcgcgg tgcagcaggt ctcgatcgtt
    12421 cccaagcgca gaatccccgg cctcatcccg gtgcggcgcg agcagctcgt aggcgtcatc
    12481 gacccgatga gcggcgcatt cctcaccgcg cgctcgccga acccgaacgg cgatctcagc
    12541 gtctgcaatc agatgctgcc cgtgttcgac ggcaggtcgc gcttcgatct cgtgctcaag
    12601 ccgaagaagc gcgtcatggt gcagaaccgg tcgcgttcct cctactcggg accggcggcg
    12661 gtgtgccggg tgaagttcgt tccgatctcc ggatataggc cggacgatgc gggaattcag
    12721 cacatgaagc agtccaacga aatcgaggtc tggctgatgc cggtcagagg cacggacatg
    12781 tatgtgccct atcgtattgt cctgccgaca cccgtaggct acgggactgc ggtcatcacc
    12841 tcgatcaagg tttcaggcgc acggcgggcg agcgtcgact aacgaacagg caccgggagg
    12901 caggccctcc atcgcaacga tcgttaacgc ggaaacgcca aaccccaaca tgtcgtaggg
    12961 aacgaagctc gaaaatttac gatcccctga ttcgtccgcg ctctgttccc gttgcgatcc
    13021 ggcgagccaa gaagcgcacg gggttaacgc gaaagggcca aaccccaaca tgtcgtaggg
    13081 aacggagctc gaaaatttac gatcccctga ttcgtccgtg ttctgttccc gttgcgatcc
    13141 catggaacgg gtgatcaagg gagaccgaaa cccagatgta gcgtacaacc accaggaaac
    13201 gccgatggcg ccccctgacc ccggagaccc ggccaaactg tgttgttttg aagcagaaca
    13261 ttatccgaat gggcgatgta tgcgattcgg acgagattcg ttcttccccc gttcacgcct
    13321 gaagcgagag tcgcgttaag acctgcgtag catgaccagc tctttatcca cgcaatccac
    13381 aagtctggcc aagggccgcg gcgtcaccgc ggtgctcggg cccaccaata ccggcaagac
    13441 gcatctggcc atcgagcgca tgctcgcaca cgaggtgggc gtgatcggct tgcctttgcg
    13501 gctcctggcc cgcgaggtct acgacaaggt cgttgcccgg gcgggggccg agcaagtggc
    13561 gctcatcaca ggcgaggaga agatcaagcc gccagccgca cgcttctatg tgtgcaccgt
    13621 ggaagcaatg ccacgggagc tcgaagcgga cttcgtggcc gtcgatgaga tccagctcgc
    13681 ggccgacggc gagcgcggcc atgtcttcac caaccgcatg ttccacgcgc ggggcttgag
    13741 cgaaacgctg ctgctcggct cggcgaccat gcgcgacgcc atccgcgagc tgctccccgg
    13801 cgccaatctg gtgtcgcgcc cgcggctctc caagctgtcc tatgccggcg acaagaagat
    13861 cacccgcctg ccccgccgtt cggcgatcgt cgccttctcg gccaacgacg tatatgccat
    13921 cgccgaattg gtgcggcggc agcgcggcgg cgcggccgtg gtactcggcg cgctcagccc
    13981 ccgcacgcgc aacgcgcaag tggcgctcta tcagaatggc gacgtggact ttctggtggc
    14041 caccgacgcg attgggatgg gcctgaacct cgatctcgat catgtcgcgt tcgccggcct
    14101 tcgcaagttc gacggccatt cgcatcgcaa tctcacgccc tcggaggtcg gccagatcgc
    14161 cggccgcgcc ggacggcaca tgaacgacgg cacgttcggc gtgaccggaa aggtggagcc
    14221 gctggagtcg gagctggttg agcggctgga ggaccacgct ttcgatcaag tcggcgtcct
    14281 gcaatggcgc agccgcagcc tcgatatggc ctcgctcgaa gcactgaaag agagtttgcg
    14341 tgaagcgccg aaccatccgc ggctgacccg ctcgcgcatg gtggacgacg tgatcgcctt
    14401 ggagaacgtc acccaggagg cagacatctg cgccatggcg gcggcacccg ccgcggtcgg
    14461 gctcttatgg gaaatgtgcc aaatcccgga ctaccgcaag gtttcgccga acagtcatgc
    14521 agacttggtt gccacccttt atcgcttcgt gatgagcgac gaaggaaaga tccccgaaga
    14581 ttggtttgcc aaccaggtgg cccagtctga ccgcacggac ggcgatatcg acacgctgtc
    14641 gaaccgcctg gcacaaatta ggacctggac ttttgtctcc cacaggcctc aatggctcca
    14701 tgatccagag cattggcaaa gacgcaccag ggcgatcgag gatacgcttt cagacgcgct
    14761 tcatgagcgc ctggcacaac gtttcgtgga ccggcgcacc agcgtgctca tgcggcgcct
    14821 gcgcgataag gaggagatga tggccgagat cagcgacgac ggtcagattc ttgtcgagaa
    14881 tcactatgtc ggacgccttg acggcttttg ctttacgccc gatgcgtcga gcgacggcat
    14941 tgacgggaag gccgcgcgcc atgcggcggt gcaagtgctc ggaacggagc ttgccaaccg
    15001 caccgcggcg cttgtcgcgg ctcccgacga gaccatcgcg cttgacccgc acgggcggat
    15061 cgtctggcaa ggctccggga tcgcgcggct ggagcgcggc gaaacggcgc tgaagccccg
    15121 catacacctc ttcgtcgacg agcatctgag cccgccggac cgcgaacgta tcgcagcccg
    15181 cctcgatgcc tggctggccg cccatctcga cgccaaactg aaaccgctga tcgcgctgag
    15241 cgaggcgcag gacctgacag gattggcccg cggtcttgcc ttccgcctga ccgagacgct
    15301 tggcgtgctc cgccgcgata cggccgcgga ggaaatcagg tcactcgacc aagccgaccg
    15361 gggccagctg cgcaaatacg gcgtgcgctt tggcgccttc aatctctatt tcccggccct
    15421 gctgaagccc gccgccgccg agcttctcct tctgctctgg gcgctgcacg atggcgacgc
    15481 acatggtctc aacgtcgacg gcctgcccga gcggccgcag caggggctca cgtccgtcgc
    15541 cgctgacaat gctatccccg agccttactg gcgcgccgca ggcttccacg ttgccggcag
    15601 tcgcgccgtg cgcatcgaca tgctggagcg gttgtccgat ctgatccgcg cgcgcattac
    15661 ctggcgcccc accgagaacg ccgaagacgc accacccaca ggtgccaccg gcgacggcgg
    15721 cttccgtgcg gtgcctgact tgatgtcgat ggt
//
LOCUS       DBNHVU010000021         8074 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_232014, whole genome shotgun sequence.
ACCESSION   DBNHVU010000021 DBNHVU010000000
VERSION     DBNHVU010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8074)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8074)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8074
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_232014"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="AC16Y7_00820"
     CDS             <1..759
                     /locus_tag="AC16Y7_00820"
                     /inference="COORDINATES: protein motif:HMM:NF014068.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HIR5707951.1"
                     /translation="DDFKGRYPALLDWVRRRYHDGSAIYSACSGSVMLAATGLLDNKE
                     ATSHWGYADLFRKHYPQVRFRPEPALVFADPAGHIVTAGGTTSWHDLAIHIIARHLSP
                     GEAMRIAKVYLLKWHNEGQLPYAALVRQQPHADSVVRQCEDWLEANCRAADAVTQVVA
                     VSGIPERTLKRRFKAATGTTLIGYVQNLRVEEAKRQLETEAMSFEAIAADLGYENLAF
                     FRRLFKRATGLTPGQYRKMFRPLMGANDAPKPRA"
     gene            complement(756..1316)
                     /locus_tag="AC16Y7_00825"
     CDS             complement(756..1316)
                     /locus_tag="AC16Y7_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 domain-containing protein"
                     /protein_id="HIR5707952.1"
                     /translation="MIRECIVTTMGDEGRVHIAPLGLIEDGDGWVIGPFRPSTTLDNL
                     RTTPFAVANFTDDVMVFAGCLTGNKDWPTRGAAKVPGAVLDGSLAHLELAVERVEEDE
                     LRPRFHCHIVHEETHAPFKGFNRAQAAVIETAILASRLKMLPREKIEQELAYHQIAVE
                     KTAGPREREAWRILVEKIEDFYKHAD"
     gene            complement(1342..2742)
                     /locus_tag="AC16Y7_00830"
     CDS             complement(1342..2742)
                     /locus_tag="AC16Y7_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6513 domain-containing protein"
                     /protein_id="HIR5707953.1"
                     /translation="MSENILFITGHLAEPRLKAMLSSMEADFKWSVLDIGVKVAALMT
                     EEIVLRRLPEAGAADKIMLPGRCRADLDRLSAHYGVPVVRGPDEVKDIPVFFWRARKA
                     SDMSKYDIQIFAEIIDATTMSIEEILETAEDYSHKGADVIDLGCLPDTPFPHLEDTVR
                     ALKTYGYKVSIDSADPDELLRGGKAGADYLLSLDESRLQIADEVASVPVLIPKDHGDM
                     DSLYRAMDVLDGKGLPYLADPVLDPINFGFMTSLERYADLRRRRPDVEILLGTGNLTE
                     LTDADTTGTTAMLIGIASELNIRHVLVVQVSPHTRRTYEEHDAARRLMYASHADGELP
                     KGYTDALLCLHDKRPYPSTPEEIAENADAVRDKNFRIEVAEDGVHIYNRDGHHVAQDI
                     FELYPKLDLGDDSAHAFYLAAELARAETAWRLGKRYAQDERLDWGVAADKPAEDMSKL
                     KEAGHTLAMKKTREEK"
     gene            complement(2751..3425)
                     /locus_tag="AC16Y7_00835"
     CDS             complement(2751..3425)
                     /locus_tag="AC16Y7_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid kinase family protein"
                     /protein_id="HIR5707954.1"
                     /translation="MDAPALPQGALKPRAKDAYEASIAVVKLGGSIVRAKTLENWLDV
                     IAGAAAPIVVVPGGGALADEVRTCQRDLGFGDAPAHRMALLAMDQLAWAVAGLRPGFE
                     VGETEAALRDILARGHVAVWAPHAAISGRTDLEANWRLTSDSLALWLSGQLGARACYI
                     VKSVASKRGAASAAALSRDGVVDEAFPAMLAETGIPAVMFGQGDQDALAEMLADSTAR
                     AGRAIE"
     gene            complement(3427..3831)
                     /locus_tag="AC16Y7_00840"
     CDS             complement(3427..3831)
                     /locus_tag="AC16Y7_00840"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR5707955.1"
                     /translation="MEQRLGRVSTTRETGEPLDRIFVHDYVLEVEIGVHHNEKGVTQR
                     VRFSVDIDLTPSRQELEDDIEHTLDYDYVINGIKAIIARGHINLVETLAEDVAQHCLA
                     HPRAARVTVGIEKLDKDPGAVGVEVMRSKREA"
     gene            complement(3869..4603)
                     /locus_tag="AC16Y7_00845"
     CDS             complement(3869..4603)
                     /locus_tag="AC16Y7_00845"
                     /inference="COORDINATES: protein motif:HMM:NF016367.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HIR5707956.1"
                     /translation="MSVTRFLASVRDEGEAALALAAGADIIDLKDPDNGALGAVAPPT
                     MKACLTRIAGRAPVSATVGDLPMEPGVIARAVAATAASGADDVKLGVPQGGDAGSCFA
                     ALRRLDLPAGLILVFFADAMADVDPPQAARAAGARGLMLDTAGKGDGALPDYMSIEAI
                     AHFVEAGRAAGLMVGLAGALRAKHLADLLALRPDVLGFRGGLCRDGVRNQGLDPAACG
                     RIRALIPGSSERDDGRFREAPAAALC"
     gene            complement(4626..6344)
                     /locus_tag="AC16Y7_00850"
     CDS             complement(4626..6344)
                     /locus_tag="AC16Y7_00850"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5707957.1"
                     /translation="MALIRTKICLSFAAILAVVLAVVAVAPAHAEDQVLLRAREGAAA
                     MIRGQFDKAVALYDEALATPDVSKFVQAGIYSDRGIAKWRMQKTKAAIADFNQAIELS
                     PENATVYNNRGNALMDLGHPEEALKDFDRAIALRSTYGAAYNNRGNAYAALHKYGAAF
                     QSFRKAAQFMPQSAIPFNGRGRSQTELTRYHAAVRDLTRAISLNPRYAEAYRNRAEAT
                     FAIGNYRDAAADATQAMTLEPEPPLPKLILLRAEAYARLNNFKGALADFNKVLELNPD
                     LVEAYVERGILFANNRRHDDAIGDYTRAIQIDPKNGKAHAMRGEARLKKGEPDIALYD
                     VNAALARSLGDPLALRIRGNIYEAQERVDDAIYDYQKALTKDPFQQESREALIRLGQE
                     LPVAVGQPLGEPVDGWVVTQPSPGRYIATNPDYRTLRVELEMFGSGQPAIRGWKLLGN
                     ALRGIGLLTYHAGTIGEDGDLEYVAIVDTRANKVVSIEPERWGDKPATWTWQAVSVSI
                     TDPDGHTNEVALRKARQRAAPVARRRGGDGFWGFNQSQRKKTRRRARGNRRRGGGGGG
                     FDWLFR"
     gene            complement(6437..7924)
                     /locus_tag="AC16Y7_00855"
     CDS             complement(6437..7924)
                     /locus_tag="AC16Y7_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIR5707958.1"
                     /translation="MLELLTAEEMGRADRLTIEGGTPGAVLMETASRGVADEVSARFP
                     DAETVAVLCGPGNNGGDGFVAARYLMERGYRVRLGFDGDPAKLPADAASMAARFNAPI
                     EPLGERLFTHADVIVDGLFGAGLARPIDGKLGDLIDAANASGLPIVAIDVPSGIDGTT
                     GEIRGHAIRALATVTFFRLKPGHLLQPGRLHCGPTRVVDIGIPQSVLAEIEPKTFANQ
                     PDLWLAEYPWPTVEGHKYLRGHALVASGPKDATGAARLAARGALRSGAGLVTVASPRD
                     ALEVNASQLTAVMVREVNDARGLSQLLADERKNAFLIGPGHGVGEETRAMVLAALKAS
                     PRVVLDADALTSFADDAKTLFTAIKARAAPVVLTPHEGEFVRLFGDVPGASKVDRTRT
                     AAARSGATVVLKGADTVIAAPSGEAAIDSAGTPWLATAGSGDVLGGMILGMLAQGMTG
                     FLGGCAGVWLHGEAAKVFGPGLIAEDLPEMVPQVLQKLEALRAEA"
BASE COUNT         1395 a         2675 c         2571 g         1433 t
ORIGIN      
        1 gatgacttca aagggcgcta cccggcgctt ctggactggg tgcggcgccg ttaccatgac
       61 ggcagtgcca tctactccgc ctgctccggc tcggtgatgc tcgccgcgac cgggctcctc
      121 gacaacaagg aggccacgtc acactggggc tacgcggacc tgttccgcaa acactaccca
      181 caagtgcgat ttcggcccga acccgcactg gtcttcgccg atcctgcagg ccacatcgtc
      241 accgcgggcg gcaccacctc atggcacgat ctcgctattc atattatcgc gcggcatttg
      301 agccccggcg aagccatgcg catcgccaaa gtctatctgc tgaaatggca caacgaaggt
      361 cagctgcctt acgccgcctt ggtgcgccag caaccgcatg ccgactccgt ggtccggcaa
      421 tgcgaggatt ggctcgaagc gaactgccgt gccgctgatg cggtcaccca agtcgtcgcg
      481 gtctccggta tccctgagcg cacgctcaag cggcgattca aggccgcgac aggcacgaca
      541 ctgatcggct atgtgcagaa cctgcgtgtg gaagaagcca agcggcagtt ggaaaccgag
      601 gcgatgtcct tcgaggccat cgccgccgac ctcggctacg agaacctcgc cttcttccgc
      661 cggctcttta agcgcgccac cggcctgaca ccgggtcagt atcgcaagat gtttcgccca
      721 ttgatgggcg cgaacgatgc tcccaaaccg cgcgcctagt ccgcgtgctt gtagaagtcc
      781 tcgatcttct ccacgaggat gcgccacgcc tcgcgctcgc gcgggccggc ggtcttctcc
      841 acggcgatct ggtgataggc cagctcctgc tcgatcttct cgcgcggcag cattttgagg
      901 cggctggcca ggatcgccgt ttcgatcacc gccgcttgcg cccggttgaa acccttgaac
      961 ggcgcgtggg tctcctcgtg cacgatgtgg cagtgaaagc gcggtcgcag ctcgtcctcc
     1021 tcgacgcgct cgaccgcaag ctccaaatgc gccagcgatc catcgaggac cgcgccgggc
     1081 acttttgcgg cgccccgtgt cggccaatcc ttattgccgg tgaggcagcc ggcgaagacc
     1141 atcacatcat cggtgaaatt ggcgacggca aagggcgtgg tccgcaaatt gtcgagcgtg
     1201 gtggagggcc ggaacgggcc aatgacccag ccgtcgccgt cttcgatcag accaagcggg
     1261 gcgatgtgca ccctgccttc atcgcccatg gtggtgacga tgcattcgcg gatcatcggc
     1321 atggcggcct cgcaatcact ttcatttctc ctcgcgcgtc ttcttcatgg ccagcgtgtg
     1381 gccggcctct ttaagtttcg acatgtcttc ggcgggcttg tccgccgcga cgccccaatc
     1441 gagccgttcg tcttgcgcat agcgcttgcc taagcgccac gccgtctcgg cgcgcgccag
     1501 ctcggcggca agatagaagg cgtgggcgct gtcgtcgccc agatcgagct tgggataaag
     1561 ctcgaaaatg tcctgcgcca catgatggcc gtcgcggttg tagatgtgca cgccgtcctc
     1621 ggcgacctca atgcggaagt tcttatcgcg caccgcgtcc gcgttctcgg cgatctcctc
     1681 cggcgtcgag ggataaggcc gcttgtcatg caggcaaagc agcgcgtccg tatagccctt
     1741 gggcagctca ccgtctgcgt gcgaggcata catgaggcgc cgcgcggcgt cgtgttcctc
     1801 gtaggtgcgg cgcgtatgcg ggcttacctg cacgaccaac acgtgtctaa tgttcaactc
     1861 cgaggcaatg ccgatcaaca tcgccgtggt gcccgtggtg tcggcgtcgg tcagttcggt
     1921 gagattgccg gtgccaagca aaatttcgac atcgggacgc ctgcggcgca ggtccgcgta
     1981 gcgctccaac gaagtcatga aaccgaaatt gatgggatcg agcacgggat cggcgagata
     2041 tggcagcccc ttcccgtcga gcacatccat ggcgcggtaa agggagtcca tatcgccgtg
     2101 gtccttgggg atcagcaccg ggaccgaggc cacctcgtcg gcgatctgca gccgcgactc
     2161 atcgagactg agcaaatagt cggcgcctgc tttgccgccg cgcagaagct cgtccgggtc
     2221 ggcggaatcg atgctgacct tgtagccgta tgtcttgagg gcccgtaccg tgtcttcgag
     2281 atgcggaaac ggcgtatccg gcaggcaacc caggtcgatg acgtcggcgc ccttgtggga
     2341 gtagtcctcg gcggtttcga gaatctcctc aatcgacatc gtcgtggcgt cgatgatctc
     2401 ggcgaagatc tggatgtcgt atttagacat gtcggaggct ttgcgcgcgc gccagaagaa
     2461 cacggggatg tccttgacct catcgggccc gcgtacgacc ggaacgccgt aatgcgcact
     2521 gagccggtcg agatccgcac ggcagcggcc gggcagcatg attttgtccg cggcgcccgc
     2581 ctcggggagc cgacgcaaaa cgatctcctc ggtcatcagc gcggcgacct tgacgccgat
     2641 gtcgagcaca gaccatttga agtcggcctc catgcttgac agcatggcct tgagccgcgg
     2701 ctcggccaaa tgtccggtga tgaacaggat gttctcgctc atagcgcggt ctactcgatc
     2761 gcgcgcccgg cgcgagccgt actgtcggcc aacatctcgg ccaatgcgtc ctgatcgcct
     2821 tggccgaaca tcaccgctgg tatgccggtc tcggcgagca tggcgggaaa cgcttcatcg
     2881 acgacgccgt cgcgcgacag cgccgccgcg cttgccgcgc cgcgtttgct cgcgaccgat
     2941 ttgacgatgt aacaggcgcg cgcgccaagt tggcccgaga gccacagcgc caagctatcg
     3001 gacgtcagcc gccaattggc ctcaaggtcg gtgcgcccgg agatcgcggc atgcggcgcc
     3061 caaaccgcga cgtggccgcg cgcaagaatg tcgcgcaacg ccgcctcggt ctcacccacc
     3121 tcaaagccgg ggcggaggcc cgcaacggcc caggcgagct ggtccatggc gagcaacgcc
     3181 atgcgatgcg cgggcgcatc gccaaatccg agatcgcgct gacaagtccg cacctcgtcg
     3241 gccaaggccc cgcccccggg gaccacgacg atgggcgcag ctgcacccgc gatcacatcc
     3301 agccaatttt caagcgtttt tgcgcgaaca atgctgccgc cgagcttcac cacggcgatc
     3361 gaagcctcat acgcgtcctt tgcgcgtggc ttcagcgcgc cttgcggcaa cgcgggcgca
     3421 tccatgtcag gcctcacgtt tactgcgcat gacctcgaca ccgacggcgc cgggatcctt
     3481 gtcgagcttc tcaatgccga cggtgacgcg cgccgcgcgc ggatgggcaa gacagtgctg
     3541 cgccacgtcc tccgccaggg tctccaccag attgatgtgg ccgcgcgcga taatcgcctt
     3601 aatgccgttg atgacatagt cgtagtcgag cgtgtgctca atatcgtctt cgagttcctg
     3661 ccggctcggc gtcaggtcga tgtcgacgga gaaacgcacc cgctgggtca cgcccttttc
     3721 gttgtggtgg acgccgatct ccacttcgag cacgtagtcg tgcacgaaga tgcggtcgag
     3781 cggctcgccg gtctctcgcg tggtggacac acgtcccaat cgctgttcca tggcccattc
     3841 ctgtggcgct tggttgaaag cggaagctct agcacagagc ggcggccggg gcctcccgga
     3901 aacggccatc atcgcgttcg ctggagccag ggatcagagc ccggatccgg ccgcaggcgg
     3961 cagggtcgag cccctggttc cgcacgccat cgcggcacag gcccccgcga aagcctaaga
     4021 cgtcgggccg gagcgcgaga agatcggcca agtgcttggc gcgcaaagcc ccggcgagac
     4081 ccaccatcag tccggcagcc cggccggcct ccacaaaatg ggcgatcgcc tcgatggaca
     4141 tataatcggg aagcgcgccg tcgcccttgc cggcggtatc gagcatcagc ccccgcgcgc
     4201 ccgccgcgcg cgcggcctga ggcggatcga catccgccat ggcgtcggca aagaacacca
     4261 ggatcaggcc ggcaggcaga tcgagccggc gcaaggctgc aaagcagctt cccgcatcgc
     4321 cgccttgcgg cacgccgagc ttgacgtcgt cggcaccgct ggcggccgtg gctgcgaccg
     4381 cgcgcgcgat cacgccgggc tccatgggaa gatcgcccac agtcgcactg accggcgcac
     4441 ggccggcaat gcgtgtcagg caggccttca tggtcggagg cgcaacggcg ccaagcgcgc
     4501 cattgtccgg atccttcagg tcgatgatgt cggcgccagc cgcaagcgcg agcgcggcct
     4561 cgccttcatc ccgcacactg gcgagaaagc gggtcacgga catgccgggt gcccccaagt
     4621 cggcattagc ggaacagcca atcgaagccg ccgccgccac caccgcgacg acgattgccg
     4681 cgggcccggc ggcgcgtctt cttccgctgc gactgattga atccccaaaa gccgtcgccg
     4741 ccacgccggc gcgcgaccgg ggccgcccgc tgccgcgcct tgcgcaaggc gacttcgttg
     4801 gtatgaccgt cgggatcggt gatcgacaca gagacggctt gccacgtcca cgtggcgggc
     4861 ttgtctcccc accgctcagg ctcgatggac acaaccttgt tggcgcgcgt atcgacaata
     4921 gcgacgtatt cgagatcgcc gtcctcgccg atcgtgccgg cgtgataggt caacaggccg
     4981 atgccacgca gcgcgttgcc cagcagcttc catccccgga tcgccggctg gccggaaccg
     5041 aacatttcaa gctcgacccg aagcgtgcgg taatcgggat tggtggcgat gtagcgcccc
     5101 ggcgaaggct gcgtcaccac ccatccgtca acaggctcgc ccagcggctg gcccaccgcg
     5161 accggcagtt cttgtccaag acgaatgagc gcctcgcggc tctcctgctg gaacggatcc
     5221 ttcgtcagcg ccttttggta atcgtagatc gcgtcatcca cgcgctcctg cgcttcgtag
     5281 atattgccgc gaatgcgcaa cgccagcgga tcgccgagac tgcgcgccag cgctgcattc
     5341 acgtcgtaca acgcaatgtc gggctctccc tttttgagcc tcgcctcgcc ccgcatggcg
     5401 tgggccttcc cattcttggg atcgatttga atggcgcggg tgtagtcgcc aatggcgtcg
     5461 tcgtggcggc gattgttggc gaacaagata ccgcgctcca cataggcctc gaccagatcc
     5521 ggattgagct cgagcacttt gttgaaatca gccagtgccc ctttgaagtt gttcagccgc
     5581 gcataggcct cggcgcgcag caggatgagc ttggggagcg gcggctccgg ctccagggtc
     5641 atggcttgcg tggcgtcggc ggcggcgtcc cggtaattac cgatggcgaa agtggcctcg
     5701 gcgcggttgc ggtaggcctc ggcatatctc gggttgaggc tgatggcgcg ggtcaggtcg
     5761 cgtacggcgg cgtggtagcg cgtaagttcg gtttgcgagc ggccgcgccc gttaaacggg
     5821 atcgcacttt gcggcatgaa ctgcgcggcc ttgcggaacg actggaacgc cgcgccatat
     5881 ttatggagtg ccgcataggc gttgccgcga ttgttgtaag ccgcgccgta ggtcgaacgc
     5941 agggcgatcg cccggtcgaa gtccttgagc gcttcctcag gatggccgag atccatcaga
     6001 gcgttgccgc gattgttgta gaccgtggcg ttctcgggcg agagctcgat ggcctggttg
     6061 aaatcggcga tcgcagcctt ggtcttctgc atgcgccatt tggcgatgcc ccgatcgctg
     6121 taaatgccgg cctggacgaa cttggagacg tcgggcgtcg ccaaggcttc gtcgtagagg
     6181 gccacggcct tgtcgaattg cccccggatc atcgccgcgg cgccttcgcg ggcgcgcaac
     6241 aagacctgat cctccgcgtg cgccggcgcc accgccacga cggcgagaac cacggccaag
     6301 attgccgcga agctcaagca gattttcgtg cgaataaggg ccatgccgcc gtcaaaaagg
     6361 cctttcgtca gacacttcgt cccttcatgt ctcccataag cttggagtgt gtcaataaag
     6421 ggtccccggg gccgggtcag gcttcagcgc gaagggcttc cagcttctgc aagacctgag
     6481 gcaccatctc cggcagatcc tcggcgatca agcctggacc aaagaccttg gccgcctccc
     6541 cgtgtagcca caccccggcg catcctccta agaatccggt cattccctgg gccagcatgc
     6601 ccaagatcat cccgcccagt acatcgccgg atccggcggt cgcgagccat ggcgtgccgg
     6661 cggaatcgat ggccgcctcc ccgcttggcg cggcgatcac cgtatcggca cccttgagca
     6721 cgaccgtggc gcctgaccgg gcggcggcgg ttcgcgtccg gtcgaccttc gatgcgcccg
     6781 gcacgtcgcc gaacagccgt acgaactcgc cttcgtgggg cgtcagaacc accggcgcgg
     6841 cgcgcgcctt gatggcggtg aagagcgtct tggcgtcatc ggcaaacgag gtcagcgcat
     6901 cggcatccag caccacgcgg gggctggcct tcagtgccgc cagcaccatc gcccgggttt
     6961 cttcgccgac gccgtgaccg ggcccgatca gaaatgcgtt cttgcgttcg tcggccagga
     7021 gttgcgaaag cccgcgcgca tcgttcacct cgcgcaccat gacggcggtg agctgggagg
     7081 cattgacctc caatgcatca cgaggcgagg cgacggtgac aaggccggcg cctgaccgga
     7141 gcgcaccacg cgccgcaagc cgcgcggcac ccgttgcgtc cttggggccc gaggcgacca
     7201 gggcgtggcc gcgcagatat ttgtgccctt ccaccgtggg ccaaggatat tcggcgagcc
     7261 acaaatcggg ctgattggca aaggtcttcg gctcgatctc cgcgagcacg gattgtggga
     7321 tgccgatatc gacgacgcgc gtgggtccgc agtgaagccg gcccggctgg agcaagtgcc
     7381 ccggtttcag ccggaagaag gtcaccgtgg ccagcgcgcg tatggcatgc ccgcggatct
     7441 cgccggtggt gccgtcgatc ccactcggca cgtcgatcgc cacaatggga aggccgctgg
     7501 cattggccgc gtcgatgaga tcgccgagct tgccgtcgat gggccgggcg agcccggcac
     7561 cgaacagacc gtcgacgatg acgtcggcgt gggtgaagag cctctcgccg agaggctcga
     7621 taggcgcatt gaagcgtgcg gccatggaag cggcgtcggc cgggagcttt gccggatcgc
     7681 cgtcgaagcc aagacggacg cgatagccgc gctccatcag gtaccgcgcg gccacgaagc
     7741 catcgccccc gttattgccg ggcccgcaca ggaccgcgac cgtctccgca tcggggaagc
     7801 gcgcggaaac ctcgtcggcg acgccgcggc tcgccgtctc catgagcacc gcgccgggtg
     7861 taccgccctc gatggtcagg cggtcggcac gccccatttc ctccgccgtc aaaagttcaa
     7921 gcatgtcttc cagccgttat atgcgccagg tgatgtagac cagggccgtg atgcgatagc
     7981 aatttgcggc tcgacatttc attgcccttt ctctaggcag agtgcctaat ctatatgcac
     8041 gtctattgac ctattcgcgt gcgctttcgc ggaa
//
LOCUS       DBNHVU010000022        19809 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1198407, whole genome shotgun sequence.
ACCESSION   DBNHVU010000022 DBNHVU010000000
VERSION     DBNHVU010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 19809)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19809)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19809
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1198407"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1087)
                     /locus_tag="AC16Y7_00860"
     CDS             complement(158..1087)
                     /locus_tag="AC16Y7_00860"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5707959.1"
                     /translation="MSQRVLITGASGFIGKALVPALAQAGFTVRAAARDPSGVIAPGT
                     VERVAMPDLGETADWAPLLEGVEHVVHLAGIAHAPGTLPDDVYTRVNAEAVGALAEQA
                     KGKVARLVLMSSVRAQVGLSADAPITEADTPAPTDTYGQTKLRAEQLLADSGVAYTVL
                     RPSVVYGKGVKGNIAALAAVAKTPMPLPFGGLDNARSLLAQENLVSAVAHVLKTPATE
                     GETYLVADAAPISVADLVSAMREGLGKPPHLVKMPLGAVKRMMKSFGREADWERVSGH
                     FAINPAKLMATGWQPEIETRAGIISMMRAESGA"
     gene            complement(1088..2086)
                     /locus_tag="AC16Y7_00865"
     CDS             complement(1088..2086)
                     /locus_tag="AC16Y7_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HIR5707960.1"
                     /translation="MLASWAALTAVSAGLSGALILVLKPILARHLLAHPNARSSHKAA
                     TPQGAGLAVMAALLAVCGSAILLGVAVPPPSLIPILAGAAVLTVIGALDDVHALAVSW
                     RLFAQALVAFAIVFLLPPDFRILPDLLPAMVERVLLALGVIGFINAVNFLDGLDWMTV
                     VQVVPITLGVAVLCNLDLVPSTVGFLALALLGATLGFAVFNKHPAQIFLGDAGSLPIG
                     LVLAYLLLYVAEAHIVSAILMALYTLTDSIVTFSRRLLAGEKVFEAHRVHFYQRAVAN
                     GMNVRQVTARIFWLGLWLACLAIAAALARTYTFDITALLLGLAGTGLVLFNFARGR"
     gene            complement(2102..4063)
                     /locus_tag="AC16Y7_00870"
     CDS             complement(2102..4063)
                     /locus_tag="AC16Y7_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR5707961.1"
                     /translation="MSLAVWLIERPRWFKRSLLIMNDFAMLSIALWAAYTLRLSRLYV
                     PETWQIFLLMLAAPVVGVIVFYWRGLYKLVTRFIGPEGTTRIYTAVILAALTWALVVL
                     MSGIQGHPRSVVVIYALIAAGLIRLSRQWAGSMLLKAAPHHKPVSSDERKNVIIYGAG
                     PIGIQLLRALNETSNYRPVAFIDANPSLAGQMVHGVKVARPEKISKLISDENVAEVLL
                     TTPSALRGERRLALKALEAYPVAVKTLPALEEIASGHVEVSDLRPIDVEDLLGRDSVA
                     PDTELLTAQVSGKVVMITGAGGSIGSELTRQLLQLEPRALILLDSSEAALYEISMEIE
                     ERQHRLRKESEGARNDTAILQILGSVLDRKLIDRIIEEQGVEVIYHAAAYKHVPIVER
                     NPFAGLHNNTLGTLVVAEAAKEHGVERFVLVSTDKAVRPTNIMGASKRLAELVLQGLA
                     QDNSNTIFTMVRFGNVLDSSGSVVQLFRNQIKAGGPVTVTHPDVTRYFMSIPEAAQLV
                     IQAGAMATGGEVFVLEMGTPVKIADLARTMIRLSGLDVRDETNPDGDVAIDFVGLRPG
                     EKLFEELLIGENTTGTGHPRIFKTSEPILPYDDLIAVLERMEDAIQRVDLAGVQDVLR
                     ATVEGYVPAKAPTAVSAKDEWQPISRTLH"
     gene            4291..5421
                     /locus_tag="AC16Y7_00875"
     CDS             4291..5421
                     /locus_tag="AC16Y7_00875"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR5707962.1"
                     /translation="MGRRSAISKGVSKGAVSGALGLAMVLLGSAPAVSETATFATPIA
                     YGTHLPGLGTQALTLARSLKKRSDGQIVLDLKEPGDGTLPHEILDKVSDGKVDAGFAT
                     ASFWAAKVPATPLFGGFPFGPDASGYVAWFEHGNGRALYQEMYDQAKLKVHVIPCGFG
                     GAEAGIWSKQEIADTKDLDGLRVRIFGLGARVLSRLGAVPVLVPGGQLPQAFKEDKIA
                     AAELYTPAVDRAKGLQDAVKLIYGPGWHQPATLLELLINKDRWDALGKGQQDLIESTC
                     QVMLRSSRAESAGLQAAALASLRTEDNVQIARLPDGVLKALREAWGEVAKEEGARDYF
                     FKQVLEDIAKFEAAEAAPQAPAPEALPPASEGAPKDKPDPAR"
     gene            5563..7095
                     /locus_tag="AC16Y7_00880"
     CDS             5563..7095
                     /locus_tag="AC16Y7_00880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5707963.1"
                     /translation="MSTLNRPDPDRAVLARRNDIVKQLRRLVPDAVLIADEEGRRTFE
                     SDALTAYRCLPLAVLLPGTTEEVAAILRFCQDNHIKVVPRGAGTSLCGGALPLEDAVV
                     LCLSRMNKVLEVDPKNRIARVEAGITNIDITEAASKHGFFYAPDPSSQVACTVGGNIA
                     TNSGGAHCLKYGVTVNNLLGVRMVTMDGEIIDIGGVHFDAPGYDFLPLITGSEGQLGV
                     VTEATVRLLPAPEGTRPVLIGFDSSEAAASCVAAIIAAGIVPVAIEFMDRPAIHVCEH
                     FVPAGYPLDAEAALIIEVEGSEDEIEYLLEKIEDIARDFLPTSLRRSQSPDETAMIWK
                     GRKAAFGAIGQISDYYCMDGVIPLSQLPKALDEIGWICRKYRFDVANIFHAGDGNLHP
                     LILYNANDSVQLERVELCGAEILKLCVELGGCLTGEHGVGIEKRELMYEQYTDEDIDQ
                     QVRVKEEFDPSWLLNPGKVFPIEGRDALRLRSVELQGLEVDVVTETVAVEEELVEGRD
                     AP"
     gene            7092..8327
                     /gene="glcE"
                     /locus_tag="AC16Y7_00885"
     CDS             7092..8327
                     /gene="glcE"
                     /locus_tag="AC16Y7_00885"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HIR5707964.1"
                     /translation="MTGHMRLGFHAPETEEELQHLVAEAAETRTPLEVMGTGTKRELG
                     HPIGAGAVVSTEAMTGVTLYEPTELVLVAKAGTPLTEIDAALAENDQELPFEPVDFGP
                     VLGYGPGDGTLGGMIGTNISGSRRLLKGAARDHVLGVRAVNGRGELIKAGGRVMKNVT
                     GYDLSRTLAGSWGTLAVMTEIALKVLPAQRESRTIICFGLTDQTAVEALCIATGLPYE
                     VSGALHLHADIAARMSDAEIADAEGAVTAIRVENFPASARYRASRVRQSLLAYSPALE
                     LDAERSKAFWDDVRALKMFQGRANPLWRISTKPTSAAKLIGNLLRTIDVRVAYDWSGG
                     LIWVETPPLTDGGAVDIRRTLAELGGHATLIRADDTIRAGIDVFQPLDPQTAVFSAKL
                     KHAFDPLGIFNPGRMYRDM"
     gene            8337..9713
                     /gene="glcF"
                     /locus_tag="AC16Y7_00890"
     CDS             8337..9713
                     /gene="glcF"
                     /locus_tag="AC16Y7_00890"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HIR5707965.1"
                     /translation="MQTNFNALQLGDPTIARADEILRRCVHCGLCTATCPTYVLTGDE
                     RDSPRGRIYFMKQMFEGRDVTPSTVRHVDRCLSCLGCMTTCPSGVDYMHLVDLARVRI
                     EQRGHRSPNRRMMRWFLSRTLPNPNRFKAMLVLGWFAKPFRNVFAKLGFRRMAAALAL
                     VPKGALKLKILKPKSAFNPKAPQPKRVALMLGCVQEVLAPQINQATIRLLRRHGIDVV
                     VVKDEGCCGALTHGLGREEEARAYARHNIDAWTAVMRERLLDGIIVTAGGCGTMLKDY
                     GNLLSRDRGYAERAEYVSGLARDITEFLDEIGLNPPVMWTGLKVAYHSACSLGHGQKL
                     DRLPRELLEQAGYTLTDIPEGHLCCGSAGTYNILEPELAGQLRDRKLKNIESIAPEVV
                     VTGNIGCMTQLKEGVNVPFVHTVELLDWATGGPCPPALSKLRGRANPIEALVEMAKAA
                     AKERELAS"
     gene            9793..11322
                     /gene="hisS"
                     /locus_tag="AC16Y7_00895"
     CDS             9793..11322
                     /gene="hisS"
                     /locus_tag="AC16Y7_00895"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR5707966.1"
                     /translation="MSKNDKKPGAEAADSRPEAKSPRTEARLPKGLQDTPAPEIRTTE
                     AMLGTIKGVFEAYGFEPLDTPAIEYTDALGKFLPDQDRPNEGVFSFQDEDEQWLSLRY
                     DLTAPLARYVAENYQGLPKPLRRYQVGPVWRNEKPGPGRFRQFMQFDADTVGAGSIAA
                     DAEMCMLAADTLEALGIKRGDYVVKIGSRKILDGVLERAGVSAKDKGQHLTVLRAIDK
                     LDRLGVEGVRLLLGKGRKDESGDFTKGADLDQKQIDAIIEFVEASGGDRDQTLAALKT
                     LVAGSAIGEEGVAEVTAMHDLLTAADFGSNRVVIDPSVVRGLEYYTGPVFEAELTASI
                     KDKRGQTIRLGSVVSGGRYDGLVERFTGEHVPATGVSVGVSRLLFGLQLLGKCTGDET
                     CGPVVVLVFDKDRLADYQKMVQELRGAGIRAELYLGSSGMKAQMKYADKRGAPCVIIQ
                     GGDEKERGEVQIKDLVLGAGLSKIEDRDEYKKKQAEAQFAVPEADLVAGVRKVLDRHK
                     A"
     gene            11327..12502
                     /locus_tag="AC16Y7_00900"
     CDS             11327..12502
                     /locus_tag="AC16Y7_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR5707967.1"
                     /translation="MTAESAKAFEALEGQARALLDLFARAGYEPVAPAIIQPAGVFLD
                     QIGEQVRGRTYVFTDLAGEELCLRPDLTVPVSRLYLERHPQADEKARYSYNGPAFRFQ
                     PLGPSATHPREFRQAGIECFGVPDKENADIEMIRLAVEAVKSAGLRDFSLRFGDIALF
                     YALVDALQLPKRWRNKLRHYFWRPPVFHALLDRIAQGTRPEPDTLATALADKIASADQ
                     ARAEEIVAAYLDAQGIPLAGNRSLREIAERLLDHAADLRADPLPQEVGTVIDYVLTVS
                     APPREACDKLAMIAKGAGLDIDAGLGAISRRFDRLEGEGVDLSRATFTAEFGRKLEYY
                     SGLVFQIEAEGLDETEPVAGGGRYDNLLTALGAPRPVPAVGSAIHTERLLAAVEGGA"
     gene            12502..13203
                     /gene="hisG"
                     /locus_tag="AC16Y7_00905"
     CDS             12502..13203
                     /gene="hisG"
                     /locus_tag="AC16Y7_00905"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HIR5707968.1"
                     /translation="MSSPALVIAVPSKGRLKEKAAALFEQGSMPLRVKGHERGYRGQL
                     EGLPDVEVEYTSAAEIVHQLRAGRVHLGITGEDLVRETLTDVDGTVEFLKPLGFGHAD
                     VIVAVPECWIDVASMADLEDVAEQFARSHGRRLRVATKYMNLTRRFFAKKGVTGYRIV
                     ESVGATEATPATGTSELIVDITSTGTTLKANHLKVLEDGLILQSQAHLLAAKTGVWDA
                     RARELREAVLGALSK"
     gene            13363..14067
                     /locus_tag="AC16Y7_00910"
     CDS             13363..14067
                     /locus_tag="AC16Y7_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR5707969.1"
                     /translation="MFARLALAGALLAASISAAAAAPARVVILRGGETAADGTLCEIG
                     KQRAQALQFNYLGEKAAKPLFTEDEPPAFVFAITPSTAALATPATKSWGKPVIFYSVL
                     PQANNKKTVEALNERTREAAGNILNNPALKGKTVVMVWERTHIADKALDAKYEREASV
                     TLRQLFHLDILPGVPREWPEDNHDYFWILDFPENSNVPVKFEMVKQEFGKSFPKVPAN
                     DWGEPSGLAPDSGCAP"
     gene            complement(14141..15781)
                     /gene="groL"
                     /locus_tag="AC16Y7_00915"
     CDS             complement(14141..15781)
                     /gene="groL"
                     /locus_tag="AC16Y7_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004109730.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR5707970.1"
                     /translation="MAAKDVKFGAEARERMLKGVDVLADAVKVTLGPKGRNVIIEKSF
                     GAPRSTKDGVTVAKEVELEDKFENMGAQMLKEVAQKTNETAGDGTTTATVLAQAIVRE
                     GLKAVAAGMNPMDLKRGIDKAVTEVVAAIERQSKEVSGSSEIAQVGTISANGEKAIGD
                     MIAQAMKKVGNEGVITVEEAKSLDSELEVVEGMQFDRGYISPYFVTNAEKMTTELENP
                     FILIYEKKLSGLQAMLPLLESVVQSGRPLLIIAEDVEGEALATLVVNKLRGGLKVAAV
                     KAPGFGDRRKAMLQDIAILTGGEMVSEDLGIKLENVTTQMLGKAKRVTITKDDTTIVR
                     GGGKKDDIEARVAQIRAQIEDTASDYDREKLQERLAKLAGGVAVIKVGGATEVEVKEK
                     KDRVDDALNATRAAVEEGIVPGGGVALLHATKALKTKGDNDDQEAGVNIVRRALQAPI
                     RQIAENAGVEGSIVVGKVLEQKSPSFGYDAQKDTYVDLISKGIIDPAKVVRTALQDAA
                     SVAGLLVTTEAGVAESPKKDEPAMPGMPPGGMGGMDMM"
     gene            complement(15869..16156)
                     /locus_tag="AC16Y7_00920"
     CDS             complement(15869..16156)
                     /locus_tag="AC16Y7_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR5707971.1"
                     /translation="MKFRPLHDRVVVKRLEEEQTTKGGIIIPDTAAEKPQTGKVVSAG
                     PGARGEDGKLQPLGVKAGDKVLFGKWSGTEVKIDGDDLLIMKESDIMGILG"
     gene            complement(16311..17288)
                     /locus_tag="AC16Y7_00925"
     CDS             complement(16311..17288)
                     /locus_tag="AC16Y7_00925"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0042542 - response to hydrogen peroxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HIR5707972.1"
                     /translation="MSGVARVSFCAIGALILIGTALSAKAEPGAAHLVDALNSVFGEH
                     PHTRAGHAKGVCVTGNFTPSAEAPSLSKAPHFAKTVPVIGRFSMGGGNPEAPDNAKGN
                     VRGMALRFELGEGATTDLAMISAPVFGAKTPQAFFDLLTARASGDADSVEAVFAANPE
                     SRNQGVWLNARPVPASYAGVNYWGVHAFTLTNAAGKETLVKFKALPAAGEEGLTDEEA
                     EAKGTNFLIGELKERLGQAPAKIDLVAIIGEDGDPTDDPTAFWDEDDRKNAALGTVSI
                     EAAAPDAICDPFSFLPSNLAEGITGPTNDPLFQIRSPAYLVSFTRRLAP"
     gene            17580..19190
                     /locus_tag="AC16Y7_00930"
     CDS             17580..19190
                     /locus_tag="AC16Y7_00930"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR5707973.1"
                     /translation="MLDVIVLIAMAVTAVAFAAGLTVQAGLPLVPVLIGTAALFLVMA
                     TSFLSMARGSRGGAASGGDRLAELEEALEIIDGDLQRLDQVEDGLSRLDTLSETVARL
                     DQTGGASGEGAGGAELEGVYSQIEALRAEIAAATAGSRTAQAFKPTSEPPEPVVSVPE
                     MPPAPPPLPEPEPVPVEKPFSLMDREPEPEPEAETLIVEEVVETVVIEPEPEVSAALQ
                     DEPQDEPQDEPEDEPKDEPQDDEAIEEVAVAETVESEPEPQSEPEPESETDLPAAALW
                     DDDEEAAEERMLGILREGISEGRVDLYLQPTITLPDRKVRYFEALTRIRKTDDEIVLP
                     GEYMPAAKRSGLMPLIDNVLLVRSVQVLRRVGQDTRIKGVFGNVSMHSLLDPDYYPEL
                     IEFMEENTSLSDNLIFEISQMEMLGLTQSELACLDTLGALGYTFCLDNVSDMETDFST
                     LSDRYFRHVKVSAASFLGGPGRKASDLKRQLDGLGMQLIIEKVENEGDVAELLDHGIE
                     LAQGYLFGEPRPMNAALSKELEDAGASQ"
     gene            complement(19241..19495)
                     /locus_tag="AC16Y7_00935"
     CDS             complement(19241..19495)
                     /locus_tag="AC16Y7_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707974.1"
                     /translation="MRHLTLKTCIAGACALGFVAFGVPAMAADQEDLALERDLRPDEE
                     PIVLEDEGVKPKEAMPAESSGGSDANIEDEMIDKIGPGAE"
BASE COUNT         3514 a         6347 c         6383 g         3565 t
ORIGIN      
        1 atgtggaagc cttggccgat gccatggcgc gcctgacaga agatgcggct ttgcggcaga
       61 cgttcggcaa agcggcgcgc agtctggtgg aggcggagtt ctcggccgac gcgatcggcc
      121 gccaaatcgt ggcgctctac aatcggctca tcggccgcta agcgccgctc tcggcgcgca
      181 tcatgctgat gatccccgct ctggtctcga tctcaggctg ccagccggtc gccatcagtt
      241 ttgccggatt gatggcgaag tgccccgaga cgcgctccca gtcggcttcg cggccgaagg
      301 acttcatcat gcgcttgacc gcacccagcg gcatcttgac cagatgcggc ggtttgccga
      361 gcccttcgcg catggcgctc acgagatcgg ccacgctgat cggcgccgca tcggccacga
      421 gataggtctc gccttcggtc gcgggcgtct tcaagacatg tgcgaccgcc gagaccaggt
      481 tctcctgggc gagcagcgag cgcgcattgt cgagcccgcc gaagggaagc ggcatcggcg
      541 ttttggcgac ggcggcgagc gcggcgatgt tgcccttcac gcccttgccg tagaccacgg
      601 atggacgcag caccgtgtaa gcgacgccgc tgtcggcaag gagctgttcc gcccgcagct
      661 tggtctggcc ataagtatcg gtcggggccg gcgtgtccgc ctcggtgatc ggtgcgtccg
      721 ccgacagtcc cacttgcgcc cgtaccgacg acatcaggac gaggcgcgcc accttgcctt
      781 tggcctgctc ggccagcgcg ccaaccgcct cggcattgac gcgcgtatag acatcatcgg
      841 gaagcgtgcc tggtgcatgg gcaatgccgg cgagatggac cacgtgctca acgccttcga
      901 gcagcggcgc ccagtcagcc gtctcgccga gatcgggcat cgcaacgcgc tccaccgtgc
      961 ccggggcgat aacgcccgaa ggatcgcgcg cggcggcacg gacggtaaag cccgcttgcg
     1021 ccagcgcggg cacaagcgct ttgccgatga agccggacgc gccggtgatg aggacacgct
     1081 gcgacattca acgcccccgc gcgaagttga acagcacgag gcccgtaccg gctaagccca
     1141 acagaagggc cgtgatgtcg aacgtgtagg tgcgggccaa ggccgcggcg atggcgaggc
     1201 acgccagcca caggccgagc cagaatatgc gggcggtcac ttgtcgcacg ttcatcccgt
     1261 tcgcgacggc acgttgataa aagtgcacgc ggtgcgcctc gaaaaccttc tcgccggcca
     1321 gaaggcgccg cgaaaacgtg acgatgctgt ccgtgagggt gtagagcgcc atcagtatcg
     1381 cggacacaat gtgcgcctcc gccacataga gcagcaggta ggcgagcacg aggcctattg
     1441 gtaggcttcc cgcatcgccg agaaagattt gcgccgggtg cttgttgaac acggcgaagc
     1501 caagcgtcgc gccgagtagc gccaaggcta ggaaccccac agtggacggc accaggtcga
     1561 gattgcaaag gacggccacg ccaagggtga tgggcacgac ttgcaccacg gtcatccagt
     1621 cgagcccgtc caggaagttg acggcgttga taaagccgat cacgccgagc gctaaaagca
     1681 cacgttcgac catggccggc aaaaggtcgg gcaagatgcg aaaatccggc ggcagcaaaa
     1741 acacgatggc gaaggcgacc agcgcctggg caaacagccg ccacgacacg gcgagcgcat
     1801 ggacgtcgtc gagcgcacca atcaccgtca ggacggcggc gccggccaaa attggaatga
     1861 ggctgggcgg cggcacggcc acacccagca ggatcgccga gccgcacacc gcgagcaggg
     1921 cagccatcac ggcgagtccc gcgccttgag gcgttgccgc cttatgcgaa ctgcgcgcgt
     1981 tggggtgtgc gagtaggtgc cgcgcgagga tcggtttgag aacaaggatc aaggcgccgc
     2041 tgagcccggc gctgacggcc gtcaaggcgg cccaggaggc aagcacgggt gtcaccattt
     2101 gctagtgcag cgtacgtgag atcggttgcc actcgtcctt ggcggagacc gcggtgggcg
     2161 ccttggccgg cacatagccc tcgacggtag cgcgaagcac gtcctggacc ccggcgaggt
     2221 ccacgcgctg tatggcgtcc tccatgcgct ccagcaccgc gatcaaatcg tcatagggca
     2281 ggatcggttc cgacgtcttg aagatgcgcg gatggccggt gcccgtcgta ttctcgccga
     2341 tgaggagctc ttcgaaaagt ttctcgccgg gacgcaggcc cacaaagtcg atcgcaacgt
     2401 cgccgtcggg attggtctcg tcgcggacat cgagtcctga aaggcggatc atggtgcgcg
     2461 cgagatcggc aatcttcacg ggcgttccca tctccagcac gaacacttcg ccgcctgtcg
     2521 ccatggcccc ggcttggatc acaagctggg cagcttcggg gatcgacata aagtagcggg
     2581 ttacgtctgg atgcgtgacg gtcactgggc cgccggcctt gatctggttg cggaacaact
     2641 gcaccaccga gcccgaggaa tcgagcacgt tgccgaagcg caccatggtg aagatggtgt
     2701 tgctgttgtc ctgggcgagc ccctgtagga cgagttcggc aaggcgcttg ctggcgccca
     2761 tgatgttcgt gggccgtacg gccttgtcgg tcgacaccag cacgaagcgc tcgacgccat
     2821 gctccttggc ggcctcggca accaccagcg tgccgagcgt attgttgtgc agtcctgcga
     2881 acggattgcg ttcgacgatc ggcacatgtt tgtaggcggc cgcgtgatag atcacttcga
     2941 cgccttgctc ctctataatg cggtcgatga gcttgcgatc gagcacggac ccgagaattt
     3001 gcaagatggc cgtgtcgttc cgcgcgcctt cggactcttt gcggaggcga tgttgccgtt
     3061 cttcgatctc catcgagatt tcgtagagcg cggcttccga actatcgagc aggatgagcg
     3121 ctcgcggttc cagctgcagc aactgacgcg tcagctccga gccgatggac ccgccagcgc
     3181 cggtaatcat caccaccttg ccgctcacct gtgcggtcag aagttccgtg tccggtgcga
     3241 ccgagtcccg gcccagtaga tcctccacgt cgatggggcg cagatcgctg acttcgacat
     3301 ggcccgaggc gatctcctcc agcgccggga gcgtcttcac cgcgacgggg taggcctcga
     3361 gtgccttgag ggcgaggcgc cgctcgccgc gcagcgccga cggcgtggtg agcaggacct
     3421 ctgccacgtt ctcatcggag atgagtttgc tgatcttctc cggccttgcg accttgacgc
     3481 cgtggaccat ttggccagcg agcgacgggt tggcgtcgat gaaggccacc ggccggtagt
     3541 tgctcgtctc gttcagggcg cgcagaagct gaataccgat aggccccgcg ccgtagatga
     3601 tgacgttctt tcgttcgtcc gagctgacgg gtttgtggtg cggggcggcc ttgagcaaca
     3661 ttgaacccgc ccattgccgg ctcagccgga tgaggcccgc ggcgatcagg gcgtagatca
     3721 ccaccaccga acgcgggtgt ccctggatgc cgctcatcag gaccaccagc gcccaggtca
     3781 gggcggcgag gatcacggcg gtgtagatgc gggtcgtgcc ctctgggccg atgaagcgcg
     3841 tgacgagctt gtacagcccg cgccagtaga agacgatcac gccgacaacg ggcgccgcca
     3901 gcatcagcag aaagatctgc caggtctccg gcacgtagag ccggctgagg cgcagggtat
     3961 aggcggccca caacgcgatg ctgagcatcg cgaagtcgtt catgatcagc agcgatcgct
     4021 tgaaccagcg agggcgctcg atcagccaaa cggcaagact catgtcggcc gaccccgttc
     4081 cggacctaag atcagcttaa gaatatcatc cacataggga aagtgtgccg caccataccc
     4141 gaagcgtccc acgaacggaa gtaaggggtt gctggcgttt cttaagaact tttgcccgtt
     4201 ccctcgcgtt atgggttctt gggcttgagg tcgtcaggcc ttccgggtca agctccgccg
     4261 cggggtaggg acagtttcgg cagcgaggga atgggcagaa ggtccgcaat cagcaaaggc
     4321 gtctccaagg gggccgtcag tggcgccttg gggctcgcca tggtgctgct tggcagtgcc
     4381 ccggcggtga gcgagacggc cacgttcgcc acccccatcg cctacggcac gcatttaccg
     4441 ggcctcggga cgcaagcctt gacgctggcc agatcgctca aaaagcgttc ggacggtcag
     4501 atcgtgctgg acctgaagga gccgggcgac ggcacgctgc cccacgagat tctcgacaag
     4561 gtctccgacg gcaaagtcga tgcaggcttt gcgaccgcga gcttctgggc cgcgaaggtt
     4621 cccgcaacgc ccctgttcgg cgggtttccc ttcggcccgg atgccagcgg ctacgttgct
     4681 tggttcgaac acggaaacgg ccgcgcgctc tatcaggaga tgtacgacca ggcgaagctc
     4741 aaggtccatg tcatcccttg cggcttcggc ggggcggagg ccggcatctg gtccaagcag
     4801 gagattgcgg acacaaagga cttagacggc ctgcgtgtac gcatattcgg gctcggcgcc
     4861 cgcgtcctgt cgcggctggg ggcggtgccc gtacttgtgc ctggcggaca actcccccaa
     4921 gccttcaaag aggacaagat cgccgccgcg gagctctaca cgccggccgt cgatcgcgcc
     4981 aaaggcttgc aagatgcggt caagcttatc tacgggcccg gctggcatca gcccgccacg
     5041 ctactggagc ttctcatcaa caaggatcgc tgggatgcgc tggggaaggg ccagcaagat
     5101 ctcatcgaat ccacctgcca ggtcatgctg cggtccagcc gcgccgaaag cgcaggcctg
     5161 caggccgcgg ccctcgcaag cctgaggacc gaggacaatg tgcagatcgc gcgcctgccg
     5221 gacggcgtgc tcaaggcgct gcgcgaggct tggggcgagg tcgccaagga ggagggcgcg
     5281 cgcgactact tcttcaagca ggttctcgag gatattgcga agttcgaggc ggcggaggct
     5341 gccccgcaag ccccggcccc cgaagcgctc ccgcctgcgt ccgagggcgc gccgaaggac
     5401 aagcccgatc cggcgcgctg acgccaattc cccacgtttg agccattttg ggacgctaca
     5461 tatagcccat ggtgggggcg catattgccc aggccagcgc cgcgtgcgaa agactagccg
     5521 acgcttctaa ccgtaatggc cactctcgac ggcttggagc ccatgtcgac attgaaccgt
     5581 ccagatccgg atcgggccgt gcttgcgcgc cggaacgaca tcgtgaagca gctgagacgg
     5641 ctcgtgcccg acgctgtgct gatcgccgat gaggaggggc gccgcacctt cgagtccgac
     5701 gcgctgacgg cctaccgttg tctgcccctt gccgtcctgc ttcccggaac caccgaggag
     5761 gtcgcggcga tcctgcggtt ctgtcaggac aaccacatca aggttgtgcc gcgcggcgcg
     5821 ggcacctcgc tatgcggtgg cgcgctgccg ctcgaggacg cggtcgtgct ctgcttgtcg
     5881 cgcatgaaca aggtgctgga ggtcgatccc aagaaccgta tcgcgcgcgt ggaggctggg
     5941 atcaccaaca tcgacatcac cgaggcggcg tccaaacacg gtttcttcta tgcgcccgat
     6001 ccgtcgagcc aggtcgcctg caccgtgggc ggcaacatcg ccaccaattc cggcggcgcg
     6061 cattgcctca aatacggggt caccgtgaac aatctgctcg gtgtccgcat ggtgacgatg
     6121 gacggtgaaa tcatcgatat cggcggcgtg catttcgacg cccccggtta cgacttcttg
     6181 ccgttgatca ccggttcgga aggtcagctc ggggtcgtga ccgaagcgac cgtgcgtctg
     6241 ctcccggcgc cggaaggcac gcgccccgtc ctgattggct tcgattcctc cgaggcagcc
     6301 gcatcctgcg tcgccgccat cattgctgca gggatcgtgc cggtcgctat tgagttcatg
     6361 gaccggccgg ccatccatgt ctgcgagcat ttcgtgccgg cggggtatcc gctcgacgcc
     6421 gaagccgcgc tcatcatcga agtcgaaggc tccgaggacg agatcgagta tctcctggag
     6481 aagatcgagg acatcgcacg cgatttcctg ccgaccagtc tgcgccgcag ccagagtccc
     6541 gacgagaccg cgatgatttg gaaggggcgc aaggccgcgt tcggcgccat cggccaaatt
     6601 tccgactatt actgcatgga tggcgtcatc ccgctgtccc agcttccgaa ggcactcgat
     6661 gagatcggct ggatttgccg caaatatcgc ttcgacgttg ccaacatctt ccatgccggc
     6721 gacgggaacc tgcatccgct gatcctctac aacgccaacg actccgttca gttggagcgc
     6781 gtggagctgt gcggtgcgga gatcttgaag ctctgcgtcg aactcggcgg ttgcctcacc
     6841 ggagagcacg gcgtcggcat cgagaaacgc gaactgatgt acgagcaata caccgacgag
     6901 gacatcgacc agcaagtccg cgtcaaggaa gagttcgacc cgtcatggct gctcaatccc
     6961 ggcaaggtgt tccccatcga gggccgcgac gcgttgcgct tgcgcagtgt cgagctgcaa
     7021 gggctcgagg tcgacgtcgt gactgagacg gtcgcggtcg aggaagagct ggtggaagga
     7081 cgcgacgccc cgtgaccggt cacatgcgcc tcggctttca cgctcctgag acggaggaag
     7141 agcttcagca tctcgttgcc gaagctgcag aaacccgtac gccgctcgaa gtcatgggca
     7201 cggggaccaa gcgcgagctg ggacatccga tcggcgccgg cgccgtggtc tcgacggagg
     7261 ccatgaccgg cgtcacgctc tacgagccga cggagctcgt cctcgtggcg aaggcgggca
     7321 cgccgttgac ggagatcgac gccgcgctgg ccgagaacga ccaggaactt ccatttgagc
     7381 cggtcgattt cggtcctgtg ctgggctacg gaccggggga cggcacgctt ggcggcatga
     7441 tcggcaccaa tatttccggc agccggcgcc tcctcaaagg cgccgcccgc gaccacgttc
     7501 tcggtgtccg tgccgtcaat gggcgcggcg agctgatcaa ggccggcggc cgcgtgatga
     7561 agaacgtcac cggctatgat ctcagccgca cgcttgccgg atcgtggggc acgctcgccg
     7621 tcatgacgga gatcgcgttg aaagtgctcc cggcccaacg cgaatcgcga accattatct
     7681 gtttcggtct caccgaccag accgccgtcg aggcgctgtg cattgccacg gggcttccct
     7741 acgaggtgtc cggcgcactc catctgcatg cggacatcgc cgcacgcatg tccgatgcgg
     7801 agatcgcgga cgcagaaggg gcggtcacgg ccatccgggt ggaaaacttt ccggcctcag
     7861 cgcgataccg tgcgagccgc gtgcgtcaat cgctcctcgc ctatagcccg gcgctggagc
     7921 tcgatgcgga gcgaagcaag gcgttctggg acgacgtgcg ggcactgaag atgtttcaag
     7981 gacgcgccaa cccgctttgg cgcatctcaa ccaagccgac ctccgcggcg aagctgatcg
     8041 gcaatttgtt gcgcactatc gatgtgcgtg tggcttacga ttggtccggt ggtttgatct
     8101 gggtcgagac gccacctttg acggatggcg gggcggtcga tattcgccgc acacttgccg
     8161 aattgggcgg gcatgccaca ctgatccgtg cggacgacac catacgcgcc gggatcgatg
     8221 tctttcagcc gctggatccg caaacggcgg tgttttcggc gaaactcaaa cacgctttcg
     8281 atccgctcgg aattttcaat ccgggccgga tgtaccggga tatgtagcgg cgcgtcatgc
     8341 aaaccaattt caacgcactt cagcttgggg acccgacgat cgcgcgcgcg gacgagatct
     8401 tgcgccgctg cgtccattgc gggctgtgca ccgcgacgtg tccgacctac gtgctgaccg
     8461 gcgacgagcg cgacagcccg cgcgggcgca tctacttcat gaagcagatg ttcgagggtc
     8521 gcgatgtgac cccgtcgacc gtgcgtcacg tcgaccgctg cctttcgtgc ttgggctgca
     8581 tgacgacctg cccgagcggt gtcgactata tgcatctcgt cgatctcgcc cgggtgcgca
     8641 tcgagcaacg tgggcatcgg agccccaacc ggcgcatgat gcgttggttc ctcagccgga
     8701 ccctgccgaa cccgaaccgg ttcaaggcca tgctcgtgct cggctggttc gccaagccgt
     8761 tccgtaacgt gttcgccaaa ctcggtttcc gccgtatggc agcggcgctg gcgctcgtgc
     8821 ccaagggcgc gttgaagctg aagatcctca agccgaaatc cgccttcaat cccaaggcgc
     8881 cccagccgaa gcgcgtggcc ctgatgctgg gctgcgtgca ggaggttttg gcgccacaga
     8941 tcaatcaagc gacgatccgg cttctgcggc gtcacgggat cgatgtcgtg gtggtgaagg
     9001 acgagggctg ctgcggtgcg ctgacccatg gccttggccg cgaggaagaa gcgcgcgcct
     9061 atgcgcgcca caatatcgac gcctggaccg cggtcatgcg cgagcgcctg ctggacggca
     9121 tcatcgtcac ggccggcggc tgcggcacca tgctcaagga ttacggcaat ctgctctcgc
     9181 gcgaccgggg ctacgccgaa cgcgccgaat atgtctcagg gctcgcccgc gacatcaccg
     9241 agttccttga cgagatcggc ctcaatccgc ccgtgatgtg gaccggtctc aaagtggcct
     9301 accactccgc ctgctcgctc ggccatggcc agaagctgga tcggctgccg cgggagctgc
     9361 tggagcaggc cggctacacg ctgaccgata ttcccgaggg gcatctgtgc tgcggctccg
     9421 ccggcaccta caacattctg gagccggagc tggccggcca gctgcgcgac cgcaagctca
     9481 agaatatcga gtccatcgcc cccgaggtcg tcgtaaccgg caatattggc tgcatgaccc
     9541 agctcaagga gggcgtgaac gtgccttttg tccacaccgt ggagctgctg gactgggcca
     9601 cgggcggccc atgcccgccg gcgctttcca agctgcgcgg ccgcgccaat cccatcgagg
     9661 cgctggtcga gatggccaag gcggcggcca aggagcgcga gctggccagc tgacgcggcc
     9721 ttgcttgctg cccaaggtgc gatgggctag aacgcggcgg ctggagccct ttagaatcag
     9781 ccgcattttc ccatgagcaa gaacgacaaa aagcccggcg ccgaggccgc cgactctcgc
     9841 ccagaggcaa aaagtcctcg tacagaggca cgcctgccga aggggctgca ggatacgcct
     9901 gcgcctgaaa tccgcaccac cgaggcgatg ctcggcacga tcaagggcgt attcgaggcc
     9961 tacggcttcg agccgctcga tacgcccgcc atcgaatata ccgacgcgct cggcaagttc
    10021 ctgcccgatc aggatcggcc caatgagggc gtgttctcct tccaggatga agacgagcaa
    10081 tggctgtcgc tgcgctatga cctgacggcg ccgctcgccc gctacgtggc ggagaactat
    10141 caaggtctgc ccaagccctt gcgccgctat caggtgggac ccgtttggcg caacgagaag
    10201 ccgggccccg gccgcttccg tcagttcatg cagttcgatg ccgatacggt gggtgcaggg
    10261 tccatcgcgg cggacgcgga gatgtgcatg ctcgccgccg atacgctgga ggcgctcggc
    10321 atcaagcgtg gcgactacgt ggtgaagatc ggcagccgca agattctcga tggcgtgctg
    10381 gaacgggccg gcgtgagtgc aaaggacaaa ggccaacacc tcacagtgct gcgtgccatc
    10441 gacaagctcg accgacttgg tgtcgagggc gtgcgtttgc ttttaggcaa gggccgcaag
    10501 gacgagtccg gcgacttcac aaaaggcgcc gatctcgatc agaaacaaat cgacgccatc
    10561 atcgaattcg tcgaggcgtc gggcggtgat cgcgaccaga cactcgccgc cctaaagacg
    10621 ctcgtcgctg ggtccgccat cggtgaagaa ggtgtcgccg aggtgacggc gatgcacgac
    10681 ctcctgaccg cggcagattt tggctcgaac cgcgtcgtca tcgatccatc cgtcgtgcgc
    10741 gggctggagt actacacggg cccggtgttc gaagcggagc tgacggcctc gatcaaggac
    10801 aagcgcggac agaccatacg tctcggctcg gtggtgagcg gcggccgcta cgatggcttg
    10861 gtggagcgct tcaccggcga gcacgtgccg gcgaccggtg tctcggtcgg cgtgtcgcgg
    10921 ctcctgttcg gattgcagct tctcggcaag tgcacgggcg acgagacctg cggtcccgtc
    10981 gtcgtgctcg tcttcgataa ggatcgcctg gccgactatc agaagatggt gcaggagctt
    11041 cgcggtgccg gtattcgcgc cgagctttat ctcggctcct ccggcatgaa ggcgcagatg
    11101 aaatatgccg acaagcgcgg cgccccgtgc gtcatcattc agggtggcga tgagaaagag
    11161 cgcggcgagg tgcagatcaa ggatctcgtg ctcggagccg ggctctccaa gattgaagac
    11221 cgcgacgaat acaagaagaa gcaggccgag gcgcagttcg ccgtgcccga agccgatctc
    11281 gtcgctggcg tccgcaaagt cctcgaccgt cacaaggcgt gagtttatga ccgcggaatc
    11341 ggccaaagca ttcgaggccc tcgaaggcca ggcacgggcg ctgctcgacc tgttcgcgcg
    11401 cgccggctac gagcccgtgg ctccggccat catccagccg gccggggtgt tcctcgacca
    11461 gatcggtgag caggtgcgcg gacgcaccta tgtgttcacc gatcttgccg gtgaggagct
    11521 gtgcctccgg cccgacctta ccgtgccggt gtcgcggctc tacctggagc ggcacccgca
    11581 agcagatgag aaagcccgct atagctataa cggccccgcc ttccgctttc agccgctggg
    11641 gccgagcgcc acgcatccgc gcgagttccg tcaggcgggc atcgaatgtt tcggtgtgcc
    11701 cgacaaagag aacgccgata tcgagatgat ccggctcgcc gtcgaggcgg tgaagagcgc
    11761 tgggcttcgc gacttctcct tgcgcttcgg cgacatcgcg ctgttttacg cgttggtcga
    11821 tgcacttcag ctacccaaac gctggcgcaa caagctacgc cactatttct ggcgtccgcc
    11881 cgtgttccac gcgctgctcg acaggatcgc gcaaggcacg cgcccggagc cggacacgct
    11941 ggccacggcg ctggccgaca agatcgccag cgccgaccag gcgcgcgccg aggagatcgt
    12001 ggcggcgtat ctcgatgcgc aaggcatccc gcttgccggc aaccggtcct tgcgcgagat
    12061 cgcagaacgc ctgctcgatc atgcggcgga cttgcgcgcc gatccgctgc cgcaggaagt
    12121 gggtacggtc atcgactatg tcctgaccgt atcggctccg ccgcgcgagg cgtgcgacaa
    12181 gctcgcgatg atcgccaagg gtgccgggct cgacatcgac gcaggcctcg gcgcgatctc
    12241 gcgccgcttc gaccgtctcg aaggtgaggg ggttgatctt tcgcgtgcga ccttcaccgc
    12301 cgagttcggc cgcaagctcg aatattattc ggggctggtc ttccagatcg aggcggaggg
    12361 gctcgacgag actgagccgg tcgccggcgg cggccgttac gacaatcttc tgacggcgct
    12421 tggcgcaccg cgtcccgtgc ccgccgtggg ttcggccatt cacaccgagc gtttgctcgc
    12481 cgccgtggaa ggaggcgcgt gatgtcttct ccggcgcttg tcatcgccgt cccgtccaag
    12541 gggcgcctga aagaaaaggc cgccgcgctg ttcgagcagg gcagcatgcc cttgcgcgta
    12601 aagggccatg agcgcggcta tcgcggccag ctcgaagggc ttcccgacgt tgaggtggag
    12661 tacacctccg cggcggagat cgtgcatcag ctgcgggcgg gccgcgtgca cctcggtatc
    12721 accggcgagg atctggtgcg tgagaccctc accgacgtgg acggcacggt ggagttcctg
    12781 aaaccgctcg ggttcggcca tgccgatgtg atcgtcgccg taccggaatg ctggatcgac
    12841 gtggcttcca tggcggacct ggaagacgtg gccgagcaat tcgcgcggtc ccacggccgc
    12901 cgcctgcgcg ttgcgaccaa gtacatgaac ctgacgcggc gcttcttcgc gaagaagggc
    12961 gtcaccggct atcgcattgt cgagagcgtc ggagcgaccg aagcgacgcc cgccacgggc
    13021 acgtccgagc tgatcgtcga catcacctcg acgggcacga cgctgaaggc gaaccacctg
    13081 aaggtgctgg aggacggact gatcctgcaa tcgcaagcgc acctgctggc ggcgaagacc
    13141 ggcgtgtggg atgcgcgtgc gcgcgaattg cgtgaggccg ttcttggtgc gctctccaag
    13201 taattggtca tggccgggct tgtcccggcc atccatgacc tggctctctg cactccggta
    13261 aatcgtggat gcccggccca aggccgggca tgaccagagt aggtcccact tcacccttag
    13321 gtgaatggcg gtcgggtccg gtagaggcta gtctccgcag ccatgttcgc tcgtcttgca
    13381 cttgcagggg ctttgctcgc cgcctccata tccgccgccg ccgctgcgcc cgcgcgcgtt
    13441 gtcattttgc gtggcggcga gacggccgcc gacgggacgt tgtgcgagat cggcaagcag
    13501 cgggcgcagg cgcttcagtt caattatctc ggcgagaagg cggccaagcc cttgttcacc
    13561 gaggacgagc cgcccgcttt cgtctttgct atcacgccgt ccacggcggc tttggccacg
    13621 cctgcgacga agagctgggg taagcccgtc atcttctatt cggtactgcc gcaagccaac
    13681 aacaaaaaga cggtcgaggc gctgaacgag cgtacgcgcg aggcggcggg caacatcctc
    13741 aacaatcccg cgctgaaggg aaagaccgtg gtgatggtgt gggagcgcac gcatatcgcc
    13801 gacaaggcgc tcgatgccaa atatgagcgc gaggcctcgg tgacactgcg ccagctgttc
    13861 catctcgaca tcctgcccgg cgtgccgcgc gagtggccgg aggacaatca cgattatttc
    13921 tggattctcg acttccccga gaactcaaac gtgccggtga agttcgagat ggtgaagcag
    13981 gaattcggca agtccttccc caaagtcccg gcaaacgatt ggggcgagcc cagcggactg
    14041 gcccccgaca gcggctgcgc gccgtagcct aacctcaaca aaaaaggggc ggcccttaaa
    14101 gccgcccctt cgcattctca ggatgctcgc cgttgccggc ttacatcatg tccatgccgc
    14161 ccatgccgcc cggaggcatg ccaggcatgg ccggctcgtc cttcttcggc gactcggcga
    14221 cgcccgcctc ggtggtcaca aggaggccgg caacggatgc tgcatcctgc agcgccgtgc
    14281 gcacgacctt ggccgggtcg atgatgccct tcgagatcag gtcgacataa gtgtccttct
    14341 gggcgtcgta gccgaaggac ggcgacttct gctcgagcac cttgccgacc acgatggagc
    14401 cttcgacacc ggcattctcg gcgatctgtc gaatgggggc ctggagcgcg cggcgcacga
    14461 tgttgacgcc tgcctcctga tcgtcgttgt cgcccttggt cttgagtgcc ttcgtggcat
    14521 ggagcagagc cacgccgccg ccaggcacga tgccttcctc gacggccgcg cgggtcgcgt
    14581 tcaacgcgtc gtcgacgcga tccttcttct ccttcacctc gacctcggtg gcgccgccga
    14641 ccttgatcac cgcaacgccg ccggcgagct tcgccagccg ctcctgcagc ttctcgcggt
    14701 catagtcgga cgccgtgtcc tcgatctgtg cgcggatctg cgccacgcgg gcctcgatgt
    14761 cgtccttctt gccgccaccg cgaacgatgg tcgtgtcgtc cttggtgatg gtgacgcgct
    14821 tggctttgcc gagcatctgc gtcgtgacgt tctcgagctt gatgccgaga tcctcggaga
    14881 ccatctcgcc gcccgtgagg atggcgatgt cctgcagcat ggccttgcgg cgatcgccga
    14941 agcccggggc cttgacggcc gcgactttca agccgccacg cagcttgttg accacgagcg
    15001 tggccagggc ctcgccttcg acgtcctcgg cgatgatgag gagcggacgg ccagactgca
    15061 ccaccgactc caagagcggc agcatggcct gaaggccgga cagcttcttt tcgtagatga
    15121 ggatgaacgg gttctcgagt tccgtggtca tcttctccgc attggtcacg aagtagggcg
    15181 agatgtaccc gcggtcgaac tgcatgccct cgaccacttc gagctcactg tcgaggctct
    15241 tggcttcctc gacggtgatg acgccctcat tgccgacctt cttcatggcc tgggcaatca
    15301 tgtcgccgat ggccttttcg ccattggccg agatggtacc gacctgcgcg atctccgagg
    15361 agccgctgac ttctttggac tgcctctcga tggctgcgac cacctcggtc acggccttgt
    15421 cgatgccgcg cttcaggtcc atggggttca tgccggcggc gacggccttc aggccctcgc
    15481 gcacgatcgc ctgggccagc acggtcgcgg tcgtggtgcc gtcgcccgcg gtctcgttgg
    15541 tcttctgggc gacctctttc agcatctgcg cgcccatgtt ctcgaacttg tcctcaagct
    15601 cgacctcctt ggccacggtg acgccatcct tggtcgagcg cggggcgccg aacgacttct
    15661 cgatgatgac gttgcggccc ttgggaccga gcgtcacttt cacggcgtcg gccagcacat
    15721 cgacgccctt gagcatgcgc tcacgggcct cggcgccgaa ttttacgtct ttggctgcca
    15781 ttgaatcctc ttatgagttg cgaacgggtc atcctgggag cccccaaagg cgctccacag
    15841 catgtctcga agaattggtc gggtggcgtt agccgagaat gcccatgatg tcggactcct
    15901 tcatgatgag gagatcgtcg ccatcgattt tgacctcggt gcccgaccat ttgccgaaca
    15961 gcaccttgtc gccggccttc acgcctaggg gctgaagttt gccgtcttca ccacgggccc
    16021 cggggcctgc ggagacgact ttgcctgtct gcggcttttc ggccgccgtg tccggaatga
    16081 tgatcccgcc tttggtcgtc tgctcttcct cgagccgttt gaccacgacg cggtcatgca
    16141 agggacggaa tttcatggat atttgctcct gttcgctgga actgcgccct ctagcactcg
    16201 cccttagcga ctgccaatag cgccgcatgt aagggcggcc tctggccctg tcaaggcgac
    16261 gcatcggccc gaggcgccgg gggcgccccg gaaacgcgtc atgttgagcg ttacggcgcc
    16321 aggcgcctgg taaacgagac gagataggcc ggggagcgga tctggaagag cggatcgttg
    16381 gtcggcccgg ttatcccttc ggctaggttg gacggcagga aggaaaaggg gtcgcaaatg
    16441 gcgtccggtg cggcggcttc gatgctcacc gttccgagcg cggcattctt gcggtcatcc
    16501 tcgtcccaga aggcggtggg gtcatctgtc ggatcgccgt cctcgccgat gatggccacc
    16561 aggtcgatct tggccggagc ctgacccagc cgctccttca actcaccgat gaggaagttc
    16621 gtccctttgg cttcggcttc ctcgtccgtc aggccttcct cgccggcggc cggtagcgcc
    16681 ttgaacttca cgagcgtctc cttgcccgcg gcgttggtca gcgtaaaagc gtgcacaccc
    16741 caatagttga cccccgcata gctcgccggc actgggcggg cgttcagcca cacgccttgg
    16801 ttcctcgatt ccggattggc ggcgaagacc gcctcgacac tgtccgcatc gccgctcgct
    16861 cttgcggtca ggaggtcgaa gaaggcttgc ggtgtcttcg ccccgaacac cggcgcggag
    16921 atcatggcca aatccgtcgt cgcgccctcg ccgagttcga agcgcaaggc catgccgcgc
    16981 acatttcctt tggcgttgtc cggagcctct gggttgccgc cgcccattga gaaccggccg
    17041 atcaccggaa ccgtcttggc gaaatgcggc gccttcgaca gcgacggcgc ttcggctgaa
    17101 ggggtgaagt ttccggtcac gcaaacacct ttggcgtgac ccgcccgcgt atgcgggtgc
    17161 tcgccgaaca cgctattcag cgcatcgacg agatgggcag cgcctggctc ggctttggcg
    17221 ctgagtgccg tgccgatgag aatgagagca ccaatggcgc agaaagaaac gcgcgcaacc
    17281 ccggacatga ccgaaactcc tatggatcaa gcctcaagta tgaggcgagc ctaataccga
    17341 aacactgtca ccgatggtgg attctttcgt caccagatgg ccatgctggc catcgctaga
    17401 gcgaggccgc tagctggccc ggtcctcctt ggccgcgatc acgctcggcg cggcgcccaa
    17461 tttcatgttg ctttgcaacg gcctgtcgcc catgatttcg gccgcgaggg gcggcgccat
    17521 catgcaatac ggcattacca tgtggtccag gcagggcgcc ccccaaagga gtgttcgcca
    17581 tgcttgatgt gatcgtcctg atcgccatgg ccgtgaccgc ggtcgcgttt gctgccggct
    17641 tgacggtcca ggccggtctg ccgctcgtgc cggtcctgat cggcacggcc gcgctctttc
    17701 tggtcatggc cacgtcgttt ctttctatgg cacgcggatc gcgtggcggc gcggcgtctg
    17761 gcggcgaccg tctcgccgag cttgaggaag cactcgaaat catcgacggc gatctgcagc
    17821 gcctcgatca agtcgaggac ggcctctccc gtctcgacac gctctccgag acagttgcgc
    17881 gactggacca aaccggcggc gcgtcgggtg agggcgccgg cggcgccgag ctcgaaggtg
    17941 tctattcgca gatcgaggct ttgcgcgccg aaattgcggc ggccacggcg ggctctcgaa
    18001 ccgcccaagc cttcaagccc acgtccgagc cccccgagcc ggtggtttcc gtgccggaga
    18061 tgccgcccgc acctccgccg cttcccgagc cagaaccggt ccccgtagag aagcctttct
    18121 cgctgatgga ccgtgagccg gagcccgagc cagaggccga gaccttgatc gtcgaggaag
    18181 tcgtcgaaac cgtcgtgatc gagcctgagc ctgaggtttc ggcggcgctg caagacgagc
    18241 cgcaagacga accacaagac gaacccgagg atgagccgaa agacgagccg caggacgacg
    18301 aggcaatcga ggaagtcgcc gtcgcggaga ccgttgagtc cgagccggag cctcagtcag
    18361 agccggagcc ggaatcagag accgacctgc cggccgctgc tctctgggat gacgacgaag
    18421 aggcggccga agagagaatg cttggcatcc tccgcgaagg gatttcggaa ggacgcgtcg
    18481 atctctacct gcagccaacc atcaccctgc cggaccgtaa ggttcgctat ttcgaggcgc
    18541 tgacgcgcat ccgcaagacg gacgacgaaa tcgtgctgcc gggcgagtac atgccggccg
    18601 ccaagcgctc ggggctcatg ccactgatcg acaacgtgct gctcgtccgt agcgtccagg
    18661 tgctccgccg cgttggccag gacacacgca tcaaaggcgt cttcggcaac gtctccatgc
    18721 actcattgct cgacccggac tactatcccg agttgatcga gttcatggag gagaacacca
    18781 gcctcagcga caacctgatt ttcgagatca gccagatgga gatgctcggt ctgactcagt
    18841 ccgaacttgc ttgcctcgat acactgggcg ccctgggcta cacgttctgc ctcgacaacg
    18901 tgtcggacat ggagacggat ttctccactt tgagcgatcg ctatttccgc cacgtcaagg
    18961 tcagcgccgc gagcttcctc ggaggaccag gccgcaaggc ttcggacctg aagcgtcagc
    19021 tcgatggcct tggcatgcag ctcattatcg aaaaggtcga aaacgagggc gatgtcgcgg
    19081 agcttctcga ccacgggatc gagttggcgc aagggtatct tttcggcgag ccgcggccca
    19141 tgaacgcggc gctgtccaaa gagctcgaag acgccggcgc ttcacagtaa gcggcgcgcc
    19201 agatgggcgc gccggaaact ggtctcgacc gacaaatcgc ctattccgct ccggggccga
    19261 tcttgtcgat catctcgtct tcgatattgg catcgctccc gccgctcgat tccgcgggca
    19321 tggcctcttt cggcttgacc ccttcgtcct ctaagacgat cggctcctcg tcgggacgca
    19381 gatccctttc cagggcgagg tcctcttggt ctgccgccat ggcgggcacg ccaaatgcaa
    19441 caaagccaag ggcgcaagcc cccgcgatgc acgtcttcaa tgtgagatga cgcatttcag
    19501 tctccattgc cgatgggagg caccaacgcg ggtgcctatg gcctcattct acgccagcga
    19561 ccatagcaca ggccggaaag tccaagtctt aacgcgccgc gaccaggggc cgcttgaccc
    19621 cttcctcctg agcctctaac cgacccccat gaccgatgct tctctcacca ttccgatcat
    19681 cgactccgtc ggggatatcg gcgcccgcta tcgcgcttgg ctcgtcgata tctggggggt
    19741 catgcacaac ggacaggccg ccttcgcgca ggccgccgcc gcgacgcgcg ccttccgcga
    19801 gtctggcgg
//
LOCUS       DBNHVU010000023         7122 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1644957, whole genome shotgun sequence.
ACCESSION   DBNHVU010000023 DBNHVU010000000
VERSION     DBNHVU010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7122)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1644957"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="AC16Y7_00940"
     CDS             <1..252
                     /locus_tag="AC16Y7_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707975.1"
                     /translation="APAKGVLPDGRNLHVTLKPHDWWRERIAQHFGPLYPVRTLRRAR
                     AGFKTWQRGPKQTLQYHLMRAREDARYYAKRVQGKPKVQ"
     gene            complement(259..945)
                     /locus_tag="AC16Y7_00945"
     CDS             complement(259..945)
                     /locus_tag="AC16Y7_00945"
                     /inference="COORDINATES: protein motif:HMM:NF015527.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HIR5707976.1"
                     /translation="MILSHKHKFIYIKTRKTASSSIEAMLSRACGPEDVITPTTDRLM
                     TGERMPPRNYRLDHPLVPKRPLWRRLLRRPERVYHPTIGFYEHIPAWRIKAYVGDEIW
                     NSYYKFTFERNPWDKQVSWYFYKTKSKTHRPSFESFMTNKEKASVENYSLYTIDGDIA
                     VDFLGRYETLGEDLAKVLKEIGIEGDTPLPRLNVYGSGDDYRKLYTDETRKLVEDWYA
                     PEIKMFGYEF"
     gene            complement(1027..1419)
                     /locus_tag="AC16Y7_00950"
     CDS             complement(1027..1419)
                     /locus_tag="AC16Y7_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HIR5707977.1"
                     /translation="MSTDADWLLKAMVVIAAADGRLDTREVGLIQQVYEKQTGRRLTA
                     VDIAKAAQANAEGDVLAQFAAASAVLDDPIKEEIIRSAYLVLLADNRISGEERKKLKD
                     IAMALKIQEIHFGTILENLALSLQGKKD"
     gene            1582..2616
                     /locus_tag="AC16Y7_00955"
     CDS             1582..2616
                     /locus_tag="AC16Y7_00955"
                     /inference="COORDINATES: protein motif:HMM:NF018017.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HIR5707978.1"
                     /translation="MRPLRIIFLADTRPGHYHLAQGVIAALAHVRPVEVTRIEVKRKW
                     IVPTRWLRARINADTFFPPRMLRMAYRIDAEELPDADLVVSAGGETHMPNICVTRLLD
                     VPNIFCGSLLRGLEADNFSLIISSYDCDADSERHLVTLKPSPINPDALGRPKTVPCFG
                     PDRLPKLAGLLVGGKAGSFRYTSKEWERLLAFVAEISKAWGTRWLISTSRRTPDQVAD
                     RIAELAKDDSVVAKFIDFRTAGPGTLPEILSKAEVVVCTEDSSSMISEAISARLPVVG
                     VAPARHRFTDEETLYRDFLMQNGWSRVVPLAKLTPETFAKALSEIEPLHENPLDALAA
                     KLKERLPELF"
     gene            2950..4608
                     /gene="ettA"
                     /locus_tag="AC16Y7_00960"
     CDS             2950..4608
                     /gene="ettA"
                     /locus_tag="AC16Y7_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HIR5707979.1"
                     /translation="MATPQYVYVMNKLNKTYPGGKQVLKDVTLAFLPGAKIGVVGVNG
                     AGKSTLLRIMAGDEKEFVGEAWAAEGVNVGYLPQEPQLDDSKDVMGNVMEGVAEKKGK
                     LDRYNELAVNYSDETADEMAQLQDEIDAQDLWDLDSQVEQAMDALRCPAGDADVTKLS
                     GGERRRVALCKLLLAKPDILLLDEPTNHLDAESVAWLEHHLRDYAGTVVMVTHDRYFL
                     DNITEWTLELDRGAGVPYKGNYSSWLEQKHKRLDVEGKQEEAKQRTLARELDWIRASP
                     KARQAKSKARIKAYDELLAEAGKDKTGKAQITIPPGPRLGDVVIEAEGLEKGFGERLL
                     IDGLSFKLPPGGIVGVIGPNGAGKTTLFRMIVGQETPENGEIRVGDTVKLGYVDQSRD
                     ALDEKKSVYEEISEGAEVVTLGKREVPARAYVGWFNFKGGDQQKKVDQLSGGERNRVH
                     LAKMLKSGANLILLDEPTNDLDVETLSSLEAALEDFAGCAVIISHDRFFLDRIATHIL
                     AFEGDSHVEWFEGNFEDYEEDKKRRLGEDSVVPKRIKYKKFARG"
     gene            complement(4633..5526)
                     /locus_tag="AC16Y7_00965"
     CDS             complement(4633..5526)
                     /locus_tag="AC16Y7_00965"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5707980.1"
                     /translation="MAASCYVGCNLPSTSHEEYMIALRTSSARLLLMLGFFVLASFAA
                     RAEETPAQDGRLNDVTAGDHRSEKNKARDKYRHPAETLTFFGIRPDMTVVEIYPGRGW
                     YTEVLAPYLKGNGTLYAAEHPGDPSYAPLQMSLDAFDKKIKDAPELYGDVKRTKLTKE
                     GDIAPSGSADLVVTFRNVHSLMRSGSEQEAFAAMFKALKPGGVLGVVQHRGDPNVKQD
                     PKGGKGYVNEDYVIELVEKAGFELADKSEINANPRDTKDYAKGVWTLPPSLRLGDEDR
                     DKYVAIGESDRMTLKFVKPAQ"
     gene            complement(5580..6194)
                     /locus_tag="AC16Y7_00970"
     CDS             complement(5580..6194)
                     /locus_tag="AC16Y7_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007816184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5707981.1"
                     /translation="MGINRRFFFDFARRVLFKGRMRQRQVDGLSFILDTWEAHYARKD
                     DRWLAYALGTTHHETGATMQPIKEYGGRRYFFHMYDIEGDRPRVARQLGNTQPGDGVL
                     FHGRGYVQLTGRSNYTKMQRKFDIDLTSSDKAANRALEPELAAKIMFYGMEKGVFTGK
                     KFADYFDEDTDRWVSARSIINPRDKPRLVADYALDYYAAISRTT"
     gene            6697..>7122
                     /locus_tag="AC16Y7_00975"
     CDS             6697..>7122
                     /locus_tag="AC16Y7_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5707982.1"
                     /translation="MSDDAQSPQAPEGLSSDQALAVLRGAGEATRLRILALLAGAELN
                     VKDLTQILAQSQPRISRHLKLMAEAGLITRFREGSWVFFRAADSGPEGALARAIVESL
                     DASDLTLARDHARAEAVQTARAEAAQSYFKAHAAEWDSIR"
BASE COUNT         1337 a         2201 c         2223 g         1361 t
ORIGIN      
        1 gcaccggcca agggcgtgct ccccgatggc cggaacctgc atgtgacgtt gaagccgcat
       61 gactggtggc gggagcgcat tgcgcagcat ttcggaccgc tctacccggt gcgcactttg
      121 cggcgggccc gcgccggctt caagacatgg cagcgcgggc cgaagcagac gctgcagtat
      181 catctgatgc gcgcgcgcga agatgcgcgg tattacgcca agcgcgtcca aggaaagccc
      241 aaggtgcagt aagcgcggct agaactcgta gccgaacatc ttgatctccg gcgcgtacca
      301 atcctcgacg agcttgcggg tctcatcggt gtagagcttc cgatagtcgt cgccgctgcc
      361 atacacattg aggcggggca ggggcgtgtc gccttcgatg ccgatctcct tcagcacttt
      421 tgcgaggtcc tcgcccaagg tttcatagcg gccgaggaag tcgacggcga tgtctccatc
      481 gatcgtgtac agcgagtaat tctcgacgct ggccttctcc ttgttggtca tgaagctctc
      541 gaacgagggc cggtgcgtct tcgacttggt cttgtagaaa taccaggaga cctgcttgtc
      601 ccaagggttc cgttcgaacg tgaacttgta atagctgttc cagatctcat cgcccacata
      661 ggctttgatg cgccacgccg gaatgtgctc gtagaagccg atcgtcgggt gatagacgcg
      721 ctcgggccgg cgtagcaggc gccgccacaa agggcgcttc ggtacgagcg gatggtcaag
      781 ccggtagttc cgcggcggca tgcgctcgcc cgtcatcagc cggtcggtgg tcggcgtgat
      841 cacgtcttca ggcccgcaag cccgcgacag catcgcctca atgctcgacg aggcggtctt
      901 gcgcgtcttg atgtagatga acttatgctt gtggctgagg atcatgggcg ttggaacggg
      961 aaccttcggg cctcacggcg gcggcgtgaa actagtcgtt tcacgccacg tcgggcaagc
     1021 gcccgattag tctttcttgc cttggagcga cagggcgagg ttttccagga tcgtgccgaa
     1081 atggatttcc tggatcttga gcgccatggc gatatccttg agcttcttgc gttcctcccc
     1141 ggaaatccgg ttatccgcta gaagcaccag ataagccgag cggataatct cttcttttat
     1201 ggggtcatcg agcacggcgg aggccgcggc gaactgcgcc agaacgtcgc cctcggcgtt
     1261 cgcctgggcg gccttggcga tatcgacggc ggtcagacgg cgcccggtct gcttctcgta
     1321 gacctgctgg atgaggccca cctcgcgcgt gtcaagccgg ccatcggcgg cggcgatcac
     1381 caccattgcc ttcaacaacc aatctgcgtc tgtgctcatc tgatcctcag ccattggccc
     1441 catcccaggg tatcctcgat cttgcggccg atagtgcggg ggcgggcgct ttttgccaag
     1501 acgggcaggt cagcaaaaca aggctttgaa gcgggcagcc aagttgcgct atgaggcggg
     1561 gtcctcttaa agacagaaac catgcgccca ctcaggatca tcttcctcgc cgatacccgg
     1621 cccggccact accacctcgc tcaaggcgtg atcgcggccc ttgcgcatgt gcgcccggtc
     1681 gaggtcacgc gcattgaggt gaagcgcaag tggatcgtgc cgaccaggtg gctgcgcgcg
     1741 cggatcaacg cagacacctt ctttccgccg cgcatgttgc gcatggccta ccgcatcgac
     1801 gcggaagagc tgccggacgc cgatctcgtg gtgtccgcgg gcggcgagac gcatatgccg
     1861 aatatctgcg tgacgcggct gctcgacgtg cccaacatct tttgcggttc gctcctacgc
     1921 ggcctggagg ccgacaattt cagcctgatc atctcatctt acgactgcga tgcggacagt
     1981 gagcggcatc tggtgacgct gaagccgtcc cccatcaatc ccgatgcact cggccgcccc
     2041 aaaacggtgc cgtgctttgg ccccgaccgg ctgccgaagc tggccggcct gttggttggt
     2101 ggcaaagccg ggagcttccg ctacaccagc aaagaatggg agcggctgct ggcattcgtc
     2161 gccgaaatct ccaaggcctg ggggacgcgc tggctgatct ccacttcccg ccgcacgccg
     2221 gaccaggtgg cggaccgcat tgccgagctc gccaaggatg acagcgtcgt ggcgaagttc
     2281 atcgacttcc gaaccgcagg gcccggcacc ctgccggaga tcctctccaa ggccgaggtc
     2341 gtcgtctgca ccgaggattc aagctcgatg atttcggagg ccatctctgc tcgcctgcct
     2401 gtggtcggcg tcgcgcccgc gcggcaccgc ttcaccgacg aggagacgct ctaccgggac
     2461 tttctcatgc aaaacggctg gtcgcgggtc gtgcccctcg ccaagctcac gccggagacc
     2521 ttcgccaagg cgctttccga gatcgagccc ttgcatgaga atccgctcga tgcgctggcc
     2581 gccaagctga aagagcgcct gccggagctc ttctagctgc tactaccgcc tataattgcg
     2641 gttgtagttc ttgcgctgct tgttgcggtt gttcttctga atctcgttac cgacgatgcc
     2701 gccaatgagc gcgccaccga ttacgccacc ggcgcgccct cgtgtgactg cggcgccgat
     2761 ccctaagccg atggcgccgc catcgccata ttgtagtaga agcgatccct cgctctattc
     2821 gggatgccgg gctcacgacg gctgcgcttg ttttggggtg gtcgtcgcgt ccgacattga
     2881 gttcgtgcgt ccgaatcgct aaaagcgcgg gcgaaacctc ctcgcaacac gaaacgatga
     2941 cggaagccca tggccacccc gcaatacgtc tacgtcatga acaagctcaa caagacctat
     3001 cccgggggga agcaggtctt gaaggacgtg acactcgcct tcctgcctgg cgccaagatt
     3061 ggcgtggttg gcgtcaacgg cgccggcaag tctacgctgc tgcgcattat ggcgggcgat
     3121 gaaaaggagt tcgtcggcga ggcctgggcc gccgaggggg tcaatgtcgg ctatctgccc
     3181 caggagcctc agctcgacga cagcaaggat gtcatgggca atgtcatgga gggcgtggcc
     3241 gagaagaagg gcaaactcga ccgttacaac gagctggccg tgaactattc cgacgaaacg
     3301 gcggacgaga tggcgcaact ccaggacgag atcgacgcgc aagacctttg ggatctcgac
     3361 agccaagtcg aacaggccat ggacgcgctg cgctgtccgg ccggcgatgc ggacgtgacc
     3421 aagctctccg gtggcgagcg ccgccgcgtc gcgctgtgca agcttctgct ggcaaaaccc
     3481 gacatcttgc tgctggatga gcccaccaac catttggacg ccgagagcgt cgcctggctg
     3541 gagcaccatt tgcgcgacta cgcgggcacc gtggtcatgg tcacccacga tcgctacttc
     3601 ctcgacaaca tcaccgaatg gacgctcgaa ctcgatcgcg gagccggggt tccctacaag
     3661 ggcaactact cgtcctggct ggagcagaag cacaagcgcc tcgacgttga gggcaagcag
     3721 gaagaggcca agcagcgcac gcttgcccgc gagctcgact ggatccgcgc aagtccgaaa
     3781 gcccgccaag ccaaatccaa ggcccgcatc aaggcctatg acgagcttct cgccgaagcc
     3841 ggcaaggaca agaccgggaa agcgcagatc accatcccgc cgggcccgcg cctgggcgat
     3901 gtcgtgatcg aggccgaggg gctggagaag ggtttcggcg agcggcttct gatcgatggc
     3961 ctgtcgttca agctgccgcc cggcggcatt gtcggcgtca tcggtccgaa cggcgccggc
     4021 aagaccacgc tattccgtat gatcgtcggc caagagacgc cggagaacgg cgagattcgc
     4081 gttggcgaca cggtaaagct cggctacgtg gatcaatcgc gtgacgcgct cgacgaaaag
     4141 aagtccgtct acgaggagat ctccgaaggc gcagaggtgg tgacgctcgg aaaacgcgaa
     4201 gtgccggccc gcgcctatgt cggctggttc aacttcaagg gcggggatca gcaaaaaaag
     4261 gtggaccagc tttcgggagg cgagcgcaac cgcgtgcact tggcgaagat gctgaagtcg
     4321 ggcgcgaacc tgatcctact cgatgagccc accaacgatc tcgacgtgga gaccctatcc
     4381 tcgctcgaag cggcactgga agacttcgcc ggctgcgccg tcatcatcag ccatgatcgc
     4441 ttcttccttg accgcatcgc cacgcatatt cttgccttcg aaggcgacag ccacgtggag
     4501 tggttcgagg gcaatttcga ggactacgag gaggacaaga agcgccgcct cggcgaggac
     4561 tcggtggtcc cgaagcgcat caagtacaag aagttcgcgc ggggctaagc agacggcacc
     4621 ggcgcgcgcg gcctattgcg caggcttcac gaatttcagc gtcatgcggt cgctttcgcc
     4681 gatcgccacg tacttgtcgc gatcctcatc gccgagacgc agcgatggtg gcagcgtcca
     4741 cacgcctttc gcataatcct tcgtgtccct cggattggca ttgatctcag atttgtccgc
     4801 aagctcgaag cccgccttct ccaccaactc gatgacgtag tcctcgttca cgtagccttt
     4861 gccgcctttg gggtcctgct tcacgttggg atcaccgcga tgctgtacga cgccaagcac
     4921 gccacccggc ttcagggcct tgaacatggc agcgaaggcc tcctgctcgg agcccgatct
     4981 catcaggcta tgcacgttgc ggaaggtgac gacgaggtcg gccgagccgg aaggcgcgat
     5041 gtccccctct ttggtcagct tcgttcgctt gacgtcgccg taaagctcgg gcgcgtcctt
     5101 tatcttcttg tcgaacgcgt cgagggacat ttgaagcggc gcgtaggatg gatcgcccgg
     5161 gtgttcggcg gcatacaagg tgccgttgcc cttgaggtag ggcgcaagaa cctccgtgta
     5221 ccagccccgg cctgggtaga tctcgaccac cgtcatgtca ggcctgatgc cgaagaaggt
     5281 gagcgtctca gccgggtgac ggtatttgtc gcgcgccttg ttcttctcag atcggtggtc
     5341 gccggcggta acgtcgttta agcgaccatc ttgtgccggt gtctcctccg cgcgcgcggc
     5401 gaacgacgcg agtacgaaaa atccgagcat gagaagcaga cgagcggagc ttgtgcgcag
     5461 agcgatcatg tattcctcgt ggctggtgga cggcagattg cagccaacgt agcagctcgc
     5521 ggccaaacac aaacccctgc gtcagttcaa gatttgttga ggtcggagcc tcaaacggat
     5581 cacgtcgtgc ggctgatggc ggcgtaataa tcgagcgcgt aatccgcgac gagtctcggc
     5641 ttgtctcgag gattgatgat cgatcgtgcc gacacccatc gatcggtgtc ctcatcgaaa
     5701 tagtcggcga atttcttgcc agtgaagacg cctttctcca tgccgtagaa catgatcttg
     5761 gcggcgagtt ccggctcgag cgcgcgattg gccgccttgt ccgagctggt caggtcgata
     5821 tcgaacttgc gctgcatctt cgtgtagttg gaccggcccg tgagctgcac atagccgcgc
     5881 ccgtgaaaaa gcacaccatc accaggttga gtgttaccga gctggcgcgc gacccgcgga
     5941 cggtccccct cgatgtcgta catgtggaag aaatagcgcc ggccgccata ctctttgatc
     6001 ggctgcatcg tggcgccggt ctcgtgatgg gtggtgccga gggcgtaggc cagccagcga
     6061 tcgtccttgc gggcgtagtg ggcttcccac gtatcgagaa tgaagcttag gccatccacc
     6121 tgccgctgcc gcatgcgtcc cttgaagagc acgcgccgcg caaaatcgaa gaaaaacctg
     6181 cgattgatcc ccacgttcgg gcctctttgg gttgccgcaa ccgaagagcg caaggctctc
     6241 gtgactgcat gttaccccga agagggccgg tggggattgc gagggggcct agatcctcaa
     6301 agggagcgct tcgaggccta ttgcctcgat ggcgggcggg tacagctgtc cccgagggcc
     6361 tcccgaacac cgtcggtgag gcgcatctcg tcgtggctcc actctccgca ccaatgctgg
     6421 ttcgtggtca tcggaaattt ctgcacgacc gtcccggtgg agggattgat ggcaatggcc
     6481 ggcggctagc ggtggcaatg ccccacagtc cgaggacggg cggtcttgtc gatgacccag
     6541 tagacgcaat gggagcatag tggccgaagg tggcgcatcg ggcgttcctt gtctgactac
     6601 tcttgagact accccgaggc ctatcactaa aattttcaga aagtctgaac ggtctccggc
     6661 ttgcgcccag tcgccagaag atataaacat atctttatgt ccgacgacgc tcagagcccc
     6721 caggcccctg aaggcttaag cagcgaccag gcccttgccg tgctccgtgg tgcgggcgag
     6781 gccacgcgct tgcgcattct ggcgctgctg gcgggcgcgg agctgaacgt caaggatctc
     6841 acccagatac tggctcagag ccagccgcgc atctcccggc acctcaaact gatggccgaa
     6901 gccgggctca tcacccgctt ccgcgagggc tcctgggtct tcttccgcgc cgccgatagc
     6961 ggcccggagg gggcgctcgc ccgtgccatc gtggaaagcc tcgatgcctc cgatttgacg
     7021 ctcgcccgcg accatgcccg tgccgaggcg gtgcagacgg cgcgcgccga ggccgcacaa
     7081 agctacttca aggcccatgc cgccgagtgg gactcgatcc gc
//
LOCUS       DBNHVU010000024         4586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1719737, whole genome shotgun sequence.
ACCESSION   DBNHVU010000024 DBNHVU010000000
VERSION     DBNHVU010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1719737"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="AC16Y7_00980"
     CDS             <1..329
                     /locus_tag="AC16Y7_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HIR5707983.1"
                     /translation="ASLLLCAGEKGMRYVLPNARVMLHQPSGGFQGQASDIERHAEDI
                     IKMKRRLNEAYVHHTGQDYDTIERTLDRDYFMSAEDSRKFGLVDEVISRRQASEGKSD
                     SAPSSS"
     gene            634..1899
                     /gene="clpX"
                     /locus_tag="AC16Y7_00985"
     CDS             634..1899
                     /gene="clpX"
                     /locus_tag="AC16Y7_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR5707984.1"
                     /translation="MSKVSGTDSKNTLYCSFCGKSQHEVRKLIAGPTVFICDECVELC
                     MDIIREENKSSLVKSRDGVPTPVEICQVLDDYVIGQGYAKRVLSVAVHNHYKRLNHSG
                     KNADVELAKSNILLVGPTGCGKTLLAQTLARILDVPFTMADATTLTEAGYVGEDVENI
                     ILKLLQAADYNVERAQRGIVYIDEVDKISRKSDNPSITRDVSGEGVQQALLKIMEGTV
                     ASVPPQGGRKHPQQEFLQVDTTNILFVCGGAFAGLEKIISARGRSTSIGFGAKVETVD
                     ERRTGDILRGIEPEDLLKFGLIPEFIGRVPVIATLDDLDEEALIRILTEPKNALVRQY
                     QRLFEMEDVQLSFAEEALRTISKKAIERKTGARGLRSIMEGILLDTMFELPSMRGVEE
                     VVISAEVVEGKARPLRIYSERHEEVGTSA"
     gene            2179..>4586
                     /gene="lon"
                     /locus_tag="AC16Y7_00990"
     CDS             2179..>4586
                     /gene="lon"
                     /locus_tag="AC16Y7_00990"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR5707985.1"
                     /translation="MTDTDPSVTIEDAPELYPVLPLRDIVVFPYMIVPLFVGREKSIA
                     ALEEVMRTDKQILLVAQKNASDDEPNADAIYEMGTLASVLQLLKLPDGTVKVLVEGMR
                     RATIARYTENENYFEAEIAPIEESLGSEDEIEALARSVVSQFESYVKLNKKISPEVLG
                     TASQIEDYSKLADTIASHLAIKIAEKQQILEVTSVSERLERVFTLMESEISVLQVERK
                     IRSRVKRQMEKTQREYYLNEQMKAIQKELGDSEEGRDDLAELEHKIEKTKLSKEAREK
                     AMGELKKLRQMSPMSAEATVVRNYLDWLLSIPWGVKGKVKRDLDDAQKILDEDHFGLE
                     KVKDRIVEYLAVQNRTNMLKGPILCLVGPPGVGKTSLGKSIARSTGREFVRMSLGGVR
                     DESEIRGHRRTYIGSLPGKVIQSMRKAKKVNPLFLLDEIDKMGADFRGDPASALLEVL
                     DPEQNHTFNDHYLEVDYDLSNVMFVTTANTLNIPPALMDRMEIIRIAGYTEDEKVEIA
                     RRHLIPEQIKNHGLEDEEFSLDDGALKEVIRRYTREAGVRSLEREIAKLSRKSVKELL
                     TTENKSVNVTIDNLADYLGVPKYRYGEAELEDQVGVVTGLAWTEVGGEILTVEGVMMP
                     GKGKMTVTGNLRDVMKESIQAANAYVRSRAVDFGIEPPLFEKRDIHVHVPEGATPKDG
                     PSAGVAMATAIISLMTGIPVKRDVAMTGEITLRGRVLPIGGLKEKLLAALRAGIKTVI
                     IPEENVKDLAEIPDSLKNALEISPVSRVEEVLKLAFTRAPQPIVWEEEGEGSAQVVPS
                     EDKSG"
BASE COUNT          996 a         1376 c         1408 g          806 t
ORIGIN      
        1 tggcctcgct gctgctctgc gccggcgaga agggcatgcg ctacgtcctg cccaacgcgc
       61 gcgtcatgct gcatcagccc tccggcggct ttcagggcca agccagcgat atcgagcggc
      121 atgccgagga catcatcaag atgaagcgcc gactgaatga agcctacgtc catcacaccg
      181 gtcaggacta cgacaccatt gagagaacgc tggatcgtga ttacttcatg tctgccgagg
      241 actcccggaa atttggcctg gttgacgagg tgatttccag gcggcaagcc tccgagggca
      301 agtcggatag cgctccgtcg tcgtcctaag gacccgaaac atcgacgcta ttggcgccca
      361 ttaggactaa cccgcgaatt gccacgatag gcccttgctg cggccataag tgacatgttc
      421 cctaggcctc aaattcattg ggaaaagcct ctttcagggg gattcgccgg attaatcaat
      481 ccttgatcag tgccgcctta gcatgctcag attgtgcgtc gggcaacctt ccaagaggag
      541 tcgctcacac ttgcgaggcg gtatcgagac gacgcgaaag cggggccaaa gcccccagcg
      601 ccaagcacga accaagaaga atcggatgat ggaatgagca aggtgagcgg aacagattca
      661 aaaaataccc tgtactgctc tttctgcggc aagagtcagc acgaggttcg caagcttatc
      721 gccggcccga cagtgttcat ctgcgatgaa tgcgttgagc tctgcatgga tattattcgc
      781 gaggagaaca agagctctct cgtgaagtct agggacggcg ttccgacccc tgtggagatt
      841 tgccaggttc ttgacgacta tgtcatcggc cagggttacg ccaagcgcgt gctctcggtt
      901 gccgtgcaca accactacaa gcggctcaat cactccggca agaacgcgga cgtcgagctc
      961 gccaagtcga acattcttct ggtcgggccc acgggttgcg gcaagacact gctcgcccag
     1021 acgctggccc gcatcctcga cgtgcccttc accatggcgg acgccacgac gctgacggaa
     1081 gccggctatg tgggcgaaga cgtcgagaac atcatcttga agttgctgca ggccgccgac
     1141 tacaacgtcg agcgggccca gcgcggcatc gtctatatcg acgaggtcga caagatcagc
     1201 cgcaagtcgg acaacccgtc catcacgcgc gacgtgtcgg gcgagggcgt ccagcaggcg
     1261 ctcctcaaga tcatggaagg cacggttgct tccgtgcccc cgcaaggcgg gcgcaagcat
     1321 ccgcagcaag agttcctgca ggtcgacacc acgaacattc tgttcgtgtg cggcggcgcg
     1381 tttgccggct tggagaagat catctccgcg cgcggccggt ccacttcgat cggcttcggc
     1441 gccaaggtgg agaccgtgga cgagcggcgc accggcgata tcctgcgcgg tatcgaaccc
     1501 gaggatcttt tgaagttcgg cctgatccca gaatttatcg gccgtgtgcc tgtcatcgcc
     1561 acgctcgacg atctggacga ggaagcgctg atccgcatcc tcaccgagcc gaagaacgcg
     1621 ctcgtgcgtc agtatcagcg tctgttcgag atggaagacg tgcagctctc cttcgccgag
     1681 gaggcattgc gcacgatctc caagaaggcc atcgaacgca agacgggcgc gcgcggcctc
     1741 cgctccatca tggagggcat tctgctcgac accatgttcg agctgcccag catgcgtggc
     1801 gtggaagagg tcgtcatcag cgccgaggtc gtggagggca aggcacggcc cttgcgcatc
     1861 tactccgaac ggcatgagga agtcggcacc agcgcctgac tttaaggccg ttaaccattc
     1921 gttcttgaag acggccgcga atcccactca aattcgggca ttcgcgcctt atccgcgcgt
     1981 gggtttgagg ccacttgacg gctccccggc cggtcgccac tttcaggttc aggcgagcca
     2041 acttgacgca gtgcccagtg gggcgtttgc cgggagaatg cacgccaaag gcggtccttg
     2101 ggggaggaga gctgagacag tagcttcgcg gaacggcact cggtccgccc aaaagacgaa
     2161 gaaagcgacg gcaccacaat gactgacaca gacccgtccg tgaccatcga ggatgcaccg
     2221 gagctttatc cggtgctgcc tctgcgcgac atcgtggttt tcccctacat gatcgtgccg
     2281 ctcttcgtcg gacgcgagaa gtcgatcgcc gccctggaag aggtcatgcg caccgacaag
     2341 cagatcctgc tggtcgcgca gaagaatgcc tcggacgacg agccgaacgc cgacgccatc
     2401 tacgaaatgg gtacgcttgc ctcggtcctg cagcttctga agctgcccga cggcacggtg
     2461 aaagtgcttg tcgaaggcat gcgccgggcc accatcgccc gctacaccga aaacgagaac
     2521 tatttcgaag ccgagattgc gccaatcgag gaaagcctcg gctcggagga cgagatcgag
     2581 gcgcttgccc gctcggtcgt gtcgcagttc gagagctatg tgaagctcaa caagaagatc
     2641 tcgccggagg tactcggcac cgcgagccag atcgaggact attcgaagct ggccgatacc
     2701 attgccagcc atctcgccat caagatcgcc gagaagcagc agatcttgga ggtcacgtcg
     2761 gtctccgagc ggttggagcg tgtcttcacg ctcatggaga gcgagatttc cgtccttcag
     2821 gtcgagcgca agatcaggag ccgcgtgaag cgtcagatgg agaagacgca gcgcgagtac
     2881 tacctgaacg agcagatgaa ggcgatccag aaggagcttg gcgacagcga ggaaggccgg
     2941 gacgacctgg ccgagctcga gcacaagatc gagaagacca agctctcgaa ggaagcgcgt
     3001 gaaaaggcca tgggcgagct gaagaagctg cgccagatga gccccatgtc ggccgaagcc
     3061 acggtcgtgc gcaactacct cgattggctg ctgtcgatcc cgtggggcgt gaagggcaag
     3121 gtcaaacgcg acctggacga cgcccagaag atcctggacg aggaccactt tggcctggag
     3181 aaggtcaagg accgcatcgt cgaatatctc gccgtccaga accgcaccaa catgctgaaa
     3241 ggcccgatcc tgtgcctggt cggtccgccc ggcgtgggca aaacctcgct cggcaagtcg
     3301 atcgcaagat ccacgggccg cgagttcgtg cgcatgagcc ttggcggcgt gcgtgacgag
     3361 tctgaaatcc gcggtcaccg ccgcacctat atcggctcat tgccgggcaa ggtgatccag
     3421 tccatgcgga aggccaagaa ggtcaatccg ctgttcctgc tcgatgagat cgacaagatg
     3481 ggcgccgatt ttcgcggcga cccggcctcg gctctgctcg aggtcctgga tccggagcag
     3541 aaccacacct tcaacgacca ctatctcgaa gtggactacg acctgtccaa cgtgatgttc
     3601 gtgaccacgg ccaacacgct gaacattccg ccggccctga tggaccgcat ggagatcatc
     3661 cgcatcgccg gctacaccga agacgaaaag gtggagatcg cccggcggca tctaatcccc
     3721 gagcagatca agaaccatgg tctagaggac gaagagttct cgctggatga cggggccttg
     3781 aaggaggtca ttcgccgcta tacgcgcgag gccggcgtcc gcagtcttga gcgcgagatc
     3841 gccaagctct cacgtaaatc ggtgaaggag ctcctgacca cggagaacaa gtcggtcaat
     3901 gtcacgatcg acaatctcgc cgactatctt ggcgtgccga aataccgcta cggcgaggcc
     3961 gagctggagg accaggtcgg tgtcgtcacc ggtctcgctt ggaccgaagt cggcggcgag
     4021 atcctgaccg tcgaaggcgt gatgatgccg ggcaagggca agatgaccgt caccggcaat
     4081 ttgcgcgatg tcatgaaaga atcgattcag gccgcaaatg cctatgtgcg gtcccgggcc
     4141 gtcgatttcg gcatcgagcc gcctttattc gagaagcggg atatccacgt tcacgtgccc
     4201 gaaggagcca ccccgaagga cggcccgtcc gcgggcgtcg ccatggctac cgccatcatc
     4261 tcgctgatga ccggaattcc ggtcaagcgg gacgtcgcca tgacgggcga gatcaccttg
     4321 cgcggccggg tcctgcccat tggcgggctc aaggagaagc tgctcgcagc cctccgcgcc
     4381 ggcatcaaga ccgtgattat ccctgaggaa aacgtcaagg atctcgcgga gatacccgat
     4441 tcgcttaaga atgcacttga aatcagcccc gtctcgcgtg tggaggaggt gctgaagctc
     4501 gccttcacac gggcgcccca acccatcgtc tgggaagaag agggcgaagg gtctgcgcag
     4561 gtcgtcccgt ccgaggacaa gtccgg
//
LOCUS       DBNHVU010000025         3984 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_679285, whole genome shotgun sequence.
ACCESSION   DBNHVU010000025 DBNHVU010000000
VERSION     DBNHVU010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_679285"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="AC16Y7_00995"
     CDS             <1..230
                     /locus_tag="AC16Y7_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HIR5707986.1"
                     /translation="SAAEHETVKSVQQALAELGYAPGPVDGDWGENTARAISAFQRDR
                     NLPQSGQITPELLGEFQRVTGRDVTKTAAHR"
     gene            257..604
                     /locus_tag="AC16Y7_01000"
     CDS             257..604
                     /locus_tag="AC16Y7_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HIR5707987.1"
                     /translation="MRLTSEIWVSAYIRRCQAAFVQAVVVRRGDAAAGAIFISIDLLD
                     GNVCLYGPAPTGITGSDTDRRWVRCLEGSAVASEEARVFLARQLDFDSDLWILEVEDS
                     AGRHFLDDALVET"
     gene            complement(903..1481)
                     /locus_tag="AC16Y7_01005"
     CDS             complement(903..1481)
                     /locus_tag="AC16Y7_01005"
                     /inference="COORDINATES: protein motif:HMM:NF018561.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HIR5707988.1"
                     /translation="MRVRLPGLYIALCVVLAGLIHIAAVLTLPMLAPRDAHARLAALG
                     PANKMIQLPLPAPGRQVMPLMAPDVRYAICRYDLSKGPVRLKARIADGNWLIAFYTPL
                     GENYYTVVGGDLKRPDVDLIVAMSDQTVAEVGVDSPEAFDNVVVVNSPVEEGIALVRV
                     PLTGQSRSVEAERALKATYCGAHKGSNQPSSP"
     gene            complement(1472..2140)
                     /locus_tag="AC16Y7_01010"
     CDS             complement(1472..2140)
                     /locus_tag="AC16Y7_01010"
                     /inference="COORDINATES: protein motif:HMM:NF018452.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HIR5707989.1"
                     /translation="MTALPDMPLTGAATRTSLWREEGSTSMRALGYFIGTFAVALILG
                     IGTAWYMIERGSPLTTMSAGPWKSWLSEGNPNADPYTRAHLARSGRLPLTSTAARYFV
                     SRTDSWGHTLSSGCEYSVVGLPLNARWWSLAVYDQYGSLIPNPSNRYSFNSEEMIRRA
                     DGSFHLNLSRNARPENWLPSGTGPDRNLVLVLRAYGVRDTDISGIGQIPADRLPKIER
                     VRCE"
     gene            complement(2177..>3984)
                     /locus_tag="AC16Y7_01015"
     CDS             complement(2177..>3984)
                     /locus_tag="AC16Y7_01015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR5707990.1"
                     /translation="AQADRVVQGGSSISQQLAKNMFLTPEKALSRKIKEAFIAIYLEN
                     HYTKPEILKLYFDRAYLGGGSYGVEAAAQYYFGKSIREVTLAEAAMLSGMFKAPTRYA
                     PHINLAASRARANEVLTNMVEAGYLSEGQVYGARLNPARIVERGDSKTPDYFLDWGFE
                     EVQRLMANRPNRILVARTTVDLGLQKAADDALKDTVNKYGRSRNFNNGALVSMETDGA
                     VRAMVGGKDYGTSQFNRAAHAYRQPGSSFKPYVYLTAIEHAGYTPDTGVSDGFVRCGR
                     WSPKNYSGGYRGRMTLRRALAKSINTVAVKLSLKAGREVVLADLEKMGIRHLKKTCSL
                     ALGDNGMTPLEHVGGYAVFAAGGLEVRPYGIEEIRTLSGELLYNHDRDEPKRKRIFKR
                     KSVEQLNSMLHTVTVAGTGRRAQLDYTNVAGKTGTSTAYRDAWFMGYTGQYVTGVWLG
                     NDDFRPMARVTGGSFPAQTWKTYMTAAYATDNIPTIPGVEPHPRQRAEQQRIAAVMSE
                     NTTAELPVPPPPENVKDMSSSTRKVLQRLGNLLKKAPALDPEDQTSPDRVQAPAPTSA
                     PSLASAANGDGISRPQPAPAATESSGQPTDAGVPQ"
BASE COUNT          708 a         1190 c         1300 g          786 t
ORIGIN      
        1 ccagtgcggc ggagcatgaa acggtgaaga gcgtgcagca agcgctcgcg gagctcggat
       61 atgcgcccgg tccagttgac ggcgattggg gagagaacac cgcgcgcgcg atcagcgcct
      121 tccagcgcga ccgcaatctt ccccaaagcg gacagatcac gccggagctg ctcggagagt
      181 tccagcgcgt cacggggcgg gacgtcacca agacagccgc gcatcgctga cggtttcgcg
      241 ttaggcttgc cacctcatgc gtcttaccag cgaaatttgg gtcagtgctt atatccggcg
      301 ctgccaggcg gcgttcgttc aggccgtggt ggtgcgccgc ggagacgcgg ctgccggtgc
      361 gatcttcatc tcgattgatc tgctggacgg gaatgtctgt ctctacgggc cggcgccaac
      421 cggaatcacc ggaagcgata cggatcggcg ctgggttcgg tgtctggagg gctccgccgt
      481 cgcatcggaa gaggccagag tgtttctggc gcggcagctc gacttcgatt cagatttgtg
      541 gatcctcgaa gtcgaagaca gtgcgggacg gcactttctt gacgatgccc tcgtcgagac
      601 gtgagcgcat cttcgcgatt aggagttcgt taaccttacc ttggtcttaa ttcggcgcag
      661 gtgcattggc gccgttgcga atcgcgctca tcgcgaaagc aaacccgtgg agactgagaa
      721 tgtcgaccat actggaattc cgccgaaccg aggagcccgc ggccaggact gacaaacctg
      781 ttgagggaac gctgggagag atcattatct ttccgggcgt ccgcatcgag cgccacacgt
      841 cgatggatgc accccaagac agctctccta gccggcggcg tgccccgcgc gtccgcaaga
      901 agtcacgggc ttgagggctg gttcgagcct ttgtgcgcgc cacaataggt ggccttgagg
      961 gctcgctccg cttctacact gcggctctgg cccgtcagtg gcacgcgcac gagggcgatt
     1021 ccctcctcca ctggcgaatt gaccaccacg acattgtcga atgcctcggg cgaatcgacg
     1081 ccgacttccg cgaccgtctg gtccgacatc gccacgatca gatcgacatc gggccgcttc
     1141 aggtcgccgc caacaaccgt gtaatagttc tcgccaagcg gcgtgtagaa cgcgatgagc
     1201 cagttgccgt cggcaatgcg cgccttcagg cgtaccggtc ccttcgaaag gtcatagcgg
     1261 cagatggcat agcgtacgtc cggagccatc aacggcatga cctgacgccc cggcgcgggc
     1321 agcggcaatt ggatcatctt gttggcaggg ccgagggccg caagccttgc gtgcgcatct
     1381 cggggcgcca gcatgggcag cgtcagcacg gcggcgatgt ggatgagacc cgccagcacg
     1441 acgcagagcg caatatagag tcctggaagc cttactcgca tcgcacacgc tcgatttttg
     1501 gcaggcggtc ggctggaatc tgaccgatac cgctaatgtc cgtgtcgcgc acaccgtagg
     1561 cgcgcaggac cagcacgagg ttgcggtctg gaccagtgcc gctcggaagc cagttttccg
     1621 gtctggcgtt tcgtgaaaga ttgagatgga agcttccgtc cgcgcggcgg atcatctctt
     1681 cgctgttgaa gctatagcga ttggaagggt tcgggatcag tgagccatat tgatcgtaga
     1741 cggcgagcga ccaccaacgc gcattcaggg gcagcccaac gacggagtac tcgcagccgg
     1801 agctcaacgt gtgcccccag ctatccgtcc gtgaaacgaa atagcgggct gcggttgagg
     1861 tgaggggaag tctgccgctt cgtgcaaggt gcgcgcgcgt gtagggatcg gcattcgggt
     1921 tgccttcgct aagccaactc ttccacgggc ccgctgacat cgtcgtcaga ggagagccgc
     1981 gttcgatcat gtaccaagcc gtgccgatac caaggatcaa ggcgaccgcg aacgtcccga
     2041 tgaaatagcc caacgcacgc atcgacgtgc tgccctcttc gcgccagagg ctggtgcgcg
     2101 ttgcggcgcc cgtcaggggc atgtctggga gggcggtcac gcgggcatcg cctttcgaag
     2161 gaagcgtctc agccgtttac tggggaacgc cggcgtcggt gggttgtccc gaactctctg
     2221 tcgcggccgg cgcgggttgg gggcgggaaa ttccatcgcc attcgccgcc gatgccaggc
     2281 tcggcgctga tgtgggcgca ggcgcttgta cgcgatcggg gcttgtctgg tcctcggggt
     2341 cgagcgccgg cgccttcttg agaaggttgc caagcctctg cagaaccttg cgggtggagg
     2401 aggacatatc cttcacgttt tcgggcggag gcggcacggg caactcagcc gtggtgtttt
     2461 cagacatgac agcagcgatc ctctgctgct cggcgcgctg gcgcggatgg ggctcgacgc
     2521 cgggaatggt cggaatgttg tcggtggcgt atgccgccgt catgtaggtc ttccaggttt
     2581 gcgcggggaa gctaccgccg gtgacgcggg ccatgggccg gaagtcatcg ttgccgagcc
     2641 agacgccggt gacatattgg ccggtgtagc ccatgaacca tgcatcgcga taggccgtac
     2701 tcgttcccgt cttgccggcg acgttcgtat agtccaattg cgcgcggcgc ccggtaccgg
     2761 ccaccgtcac ggtgtgcagc atggaattca gctgctccac ggattttctt ttgaatatcc
     2821 gcttgcgttt cggttcgtcg cgatcgtgat tgtagagaag ctcgcccgaa agcgtgcgga
     2881 tctcctcgat gccatagggc cgaacttcaa ggccgcctgc ggcaaagacg gcataaccgc
     2941 cgacatgctc aagaggcgtc atgccgttgt cgcccagcgc caaggagcac gtcttcttca
     3001 ggtgccgaat gcccatcttt tccaggtcgg cgaggaccac ttcgcggccg gcctttagag
     3061 acagcttcac ggccacggtg ttgatcgact tcgccaacgc ccgccgaagc gtcatgcgac
     3121 ccctgtagcc gcccgagtag tttttcggcg accagcgtcc gcagcgcacg aagccgtccg
     3181 agacccctgt gtcgggcgta tagccggcgt gttcgatcgc ggtgaggtag acgtacggtt
     3241 tgaacgacga tccgggttgg cggtaggcgt gcgccgcacg gttgaattgg ctcgtgccat
     3301 agtccttacc gccgaccatg gcgcgcaccg cgccatcggt ctccatcgag acgagcgcgc
     3361 cattattgaa gtttcgtgag cggccatact tgttgaccgt gtctttcaag gcgtcgtcgg
     3421 ccgctttctg taggccgaga tcgacggtcg tacgcgccac caggatgcgg ttcggcctgt
     3481 tggccatcaa ccgctggacc tcctcaaacc cccagtcgag aaaatagtca ggcgtcttcg
     3541 agtcgccgcg ctccacaatg cgagctgggt tcaggcgggc gccgtagact tggccttcgc
     3601 tcaaatagcc ggcctcgacc atgttggtca gcacctcatt ggcgcgcgcg cgcgaggcgg
     3661 cgaggttgat gtgcggggcg taccgcgtcg gcgccttgaa catgccggag agcatggcgg
     3721 cctcggccag cgtcacctcg cggatcgact ttccgaaata gtactgcgct gctgcctcga
     3781 cgccgtagga gccgccgccg agataggcgc ggtcgaagta gagcttgagg atctccggtt
     3841 tggtgtagtg gttctcaaga tagatggcga tgaacgcctc tttgatcttt ctcgacagcg
     3901 ccttctcggg tgtgaggaac atattcttgg cgagctgctg ggaaatcgag ctaccgcctt
     3961 ggacaaccct atctgcttgg gcgt
//
LOCUS       DBNHVU010000026         5902 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_85391, whole genome shotgun sequence.
ACCESSION   DBNHVU010000026 DBNHVU010000000
VERSION     DBNHVU010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5902)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_85391"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="AC16Y7_01020"
     CDS             complement(<1..557)
                     /locus_tag="AC16Y7_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HIR5707991.1"
                     /translation="MTALTKLFRTTTFRLSLTYLALFSAAAVVAIVYLYWNTTVLLTR
                     QLNQTIDAELTGLAEQYRAGGLDQLVRIVAERSRTPGNSLYLVADGDGKQLAGNLSAV
                     SPQLWDSLGPVEFFYTRPAPGGVERRLAFANVFRLPGKHRLIVGRDIEDRRELARLIR
                     TTMLWGLGVMALVGIGGGYWVSRRLL"
     gene            complement(541..1221)
                     /locus_tag="AC16Y7_01025"
     CDS             complement(541..1221)
                     /locus_tag="AC16Y7_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5707992.1"
                     /translation="MRVLVIEDDQETAAFLKRALKESGHVAEHAADGQAGLDLARTNA
                     YDVLIVDRMLPKCDGLGVIKTLRAEEVSTPVLILSALGEVDDRVKGLRAGGDDYLTKP
                     YAFSELLARIEVLARRTSPEESSTRLAVGDLVLDRLSHKVTRAGEAITLQPREFRLLE
                     YLMKHAGQVVTRTMLLENVWDYHFDPQTNVIDVHISRLRSKIDKSFGEPLLHTVRGAG
                     YTIRDGAH"
     gene            complement(1323..2885)
                     /locus_tag="AC16Y7_01030"
     CDS             complement(1323..2885)
                     /locus_tag="AC16Y7_01030"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HIR5707993.1"
                     /translation="MKTDQSPAATESPQLRKLPRKPALLAAAALIGAGVLGLSLNPSV
                     PFAVAETAQNTQTIETPFGRAPLSFADLVAKVSPAVVSINVKGDVKVADNNGSPIPGM
                     PNIPEDNPLYDFFKQFRKGMPPSQKHTPSLAQGSGFFITEDGFVVTNNHVVEDANDIT
                     VTMEDGEKFDAKLIGTDPRTDLALLKVTDKTSNKFPFVEMSDNTPRVGDWVLAVGNPF
                     GLGGTVTAGIISAHNRDIGSGPYDYLQIDAAVNRGNSGGPSFGLDGKVVGVNTAIFSP
                     SGGNVGIAFAVPAALVKEVIGELKDDGSVDRGWLGVVIQNVNDDIADSIGLADPKGAM
                     ITKVTEDGPASKEDIKAGDVIVEVNGNEVKDSRDLARKIAELDPDTKVNLSIVRYGDK
                     REVDMQLGSFPSNKKLAALKKDEPEDLSEQMKDLGLSLAPAVKIPGAGKKGVVITEVD
                     PDSDAADKGLEAGQIILQVAGVDVSDPDDVAEGIKKAMEAAKGDKDTVKVLMQVKTGD
                     QTRFVALSLKKA"
     gene            complement(3080..3559)
                     /locus_tag="AC16Y7_01035"
     CDS             complement(3080..3559)
                     /locus_tag="AC16Y7_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HIR5707994.1"
                     /translation="MTTRGPLLAFLLSLVVGLCLTVPAGAVQPDEVLDDPALEGRARA
                     LSAGLRCLVCQNQSIDDSDAPLAKDLRVLVRERLKAGDSDEEIIDFVVSRYGEFVLLK
                     PRLLPQTWALWFATPLVLLVALGVILFSYRRRKALAKSPKPLTANEKRRLKRLVNED"
     gene            complement(3561..5552)
                     /locus_tag="AC16Y7_01040"
     CDS             complement(3561..5552)
                     /locus_tag="AC16Y7_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HIR5707995.1"
                     /translation="MIAETGHYALILALGVAIVQAVLPVIGTRFRDPQLTAVAIPASQ
                     LQFILIVIAFMALTIAYVTSDFSVLNVWQNSHSAKPLLYKISGVWGNHEGSMVLWVLI
                     LALFGAAVATFGTNLPRQLQANVLAVQGSIAVAFLLFIVITSNPFVRVNPAPFDGQGL
                     NPVLQDPALAIHPPFLYAGYVGFSMAFSFAVAALIDGRIDAAWARWVRPWTLAAWMCL
                     TLGIALGSWWAYYELGWGGFWFWDPVENASFMPWLAGTALLHSALVMEKRDALKVWTI
                     LLAIITFSLSLMGTFLVRSGVLTSVHAFAVDPTRGVFILGIMAVFIGGAFALFAWRAP
                     LLSQGGIFAPISREGSLVLNNLLLTVACAAVLVGTLYPLALEALTGEKISVGAPFFNL
                     TFVPLIVPLLIAVPFGPLLAWKRGDLLAAAQRLLVAAIVSLAAIILTLAFYQKGPWLA
                     PLGIGLGVWLLMGAATDIAFRTKLFKASGAESWRRAWNLPRAAYGSALAHAGLGVMVL
                     GLVATTAWRSERIEALQPGGTVDIAGYQLKFEGVAPHQGPNYQERIGVFAVSRGGSPV
                     TKLAPSKRAFTVEKSATSEAGIYNGWRGDLYVVLGDPLKDGAYSVRVYFNPLVRFIWI
                     GALIMFFGGALSLSDRRLRVGAPKKAGKRAKSAAVPAER"
     gene            complement(5552..>5902)
                     /gene="ccmE"
                     /locus_tag="AC16Y7_01045"
     CDS             complement(5552..>5902)
                     /gene="ccmE"
                     /locus_tag="AC16Y7_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HIR5707996.1"
                     /translation="AKMEVTPGQRFRLGGLVETGSVVRDAGTTVRFAITDGAKTLPVT
                     YTGVLPDLFREGQGVVTEGTLQPDGVFHADSVLAKHDENYMPPEVAAKLKEQGVWRDD
                     SKTDGGSKSGGAVN"
BASE COUNT         1190 a         1888 c         1805 g         1019 t
ORIGIN      
        1 agcaacctcc gactcaccca atagccgccg ccgataccga ccagcgccat gacgccgagc
       61 ccccacaaca tcgtcgtgcg gatgagacgc gcaagctcgc gccgatcctc gatgtcgcga
      121 ccgacgatca gcctgtgctt gccaggcaaa cgaaagacgt tggcaaaggc aagccgccgt
      181 tccacgccac cgggcgccgg gcgcgtgtag aagaactcca caggacccaa actgtcccag
      241 agctgcggcg acactgcgct caagtttccg gcaagctgct tcccgtcccc atcggcgacg
      301 agatagaggc tgttgcccgg cgtacggctg cgctcggcaa cgatccgcac tagctgatcg
      361 agcccgccgg cgcgatactg ctcggcaagc cccgtcaact ccgcatcgat ggtctgattg
      421 agctgccgcg tcagcagcac cgtcgtgttc caatacagat acacgatcgc caccacggcg
      481 gcggcgctga acagcgcaag atacgtcagc gagagccgga aggtcgtcgt gcggaagagc
      541 ttagtgagcg ccgtcacgga tggtgtatcc agcgccgcgg acggtgtgca ggaggggctc
      601 gccgaagctc ttgtcgatct tcgaccggag ccgcgagatg tggacgtcga tgacattggt
      661 ctgcggatcg aagtggtagt cccacacatt ctccagcagc attgtgcgcg tcaccacctg
      721 ccctgcgtgc ttcatcaagt actccagcag acggaattcg cgcggctgca aagtaatggc
      781 ctcgccggcc cgcgttacct tgtgggacag ccggtcgagc acgagatcgc cgaccgcgag
      841 gcgcgttgag ctttcctccg gagaggtccg gcgggccagc acctcgatac gcgccaggag
      901 ctcgctaaag gcataaggct tggtaagata atcgtcgccg cctgcgcgca ggcccttgac
      961 ccgatcatcg acttcgccga gcgcggagag gatcaaaact ggcgtgctga cttcctcggc
     1021 ccgcagcgtc ttgatcacac caaggccatc gcatttggga agcatgcggt cgacgatcag
     1081 aacgtcgtag gcgttggttc tggcaagatc gagcccggct tgaccgtccg ccgcgtgttc
     1141 ggcgacatgg ccagactcct taagcgcccg cttgaggaag gctgcggtct cctgatcgtc
     1201 ctcgatcact agaacgcgca tgtcttgagg cctccctggg caacgacgtt acgttgagaa
     1261 gaaatggtgg gcggcgcagt cggggggaaa ccgcgccgcc ctccggcgaa agaaggctgg
     1321 ttttaagcct tcttcagcga aagcgccacg aagcgcgtct gatcgccggt cttcacttgc
     1381 atgagcacct tgacggtgtc cttgtcgccc ttagcagctt ccatggcctt cttgatgccc
     1441 tcggccacgt cgtcggggtc agagacatcc acgcccgcca cctgaaggat gatctgacca
     1501 gcctccaagc ctttgtcggc ggcatcgcta tcgggatcga cctcggtgat gacgacgccc
     1561 ttcttaccag cgccggggat cttcaccgca ggcgccagcg ataggccaag gtccttcatc
     1621 tgctcgctca ggtcttcggg ctcatccttc ttgagcgccg ccagcttctt gttgctcggg
     1681 aacgagccaa gctgcatgtc gacctcgcgc ttgtcgccgt agcggacgat ggacagattg
     1741 accttggtgt cgggatcgag ctcagcaatc ttgcgcgcaa gatcgcgaga gtcctttacc
     1801 tcgttgccgt tcacttcgac gatgacgtca ccagccttga tgtcctcttt ggaagccgga
     1861 ccgtcctcag tgaccttggt gatcatggcg cccttggggt ccgcgaggcc gatggagtca
     1921 gcgatatcgt cgttgacgtt ctggataacg acaccgagcc aaccgcggtc gacgctaccg
     1981 tcgtccttga gctcgccgat cacttccttg acgagcgctg ccggaacggc aaacgcgata
     2041 ccgacattgc cgcccgacgg ggagaagatg gcggtgttca caccaaccac cttgccgtca
     2101 agaccgaagc tcggcccacc ggagttgccg cggttcaccg cagcatcgat ctggagatag
     2161 tcgtatggac cggagccgat gtcgcggttg tgtgccgaga taatgccagc tgtaaccgta
     2221 ccgcccaggc cgaacggatt gccgacggca agtacccaat cgccaacgcg cggggtgttg
     2281 tcggacatct cgacgaacgg aaacttattg ctcgtcttgt cagtcacctt gagcagcgcc
     2341 aagtcagtgc gcgggtcggt gccgataagc ttggcgtcga acttctcgcc atcctccatc
     2401 gtcacggtga tgtcgttggc gtcctcgacc acgtggttgt tcgtaaccac aaagccgtct
     2461 tcggtgataa agaaaccgga cccttgcgcc aagcttggcg tgtgcttctg cgaaggcggc
     2521 atgcccttac ggaactgctt gaagaagtcg tagagcggat tgtcctccgg gatgttcggc
     2581 atgcccggga taggcgaccc gttattgtcg gcaaccttga cgtcgccctt gacgttgatg
     2641 ctgaccacgg cggggctcac cttagcgacg aggtcggcga aggacagcgg cgcgcgcccg
     2701 aaaggcgtct cgatggtctg cgtgttctgt gcggtctccg ccacggcgaa gggcacgctc
     2761 ggattgagcg acaacccgag cacgccggcg ccgatcagcg cggcggcagc gaggagcgcg
     2821 ggcttgcggg gtagcttccg caactgcggg ctctcggtcg cggcagggct ctggtcagtt
     2881 ttcattttca ccttctcctg agatggaaga atcccgttga tcgtgtccga cccccgatca
     2941 gacaagggcc gacgtctagg ttggaaatat agggagtccg caattacgct gcggtttcgg
     3001 cggcattaaa tttcggtaag gttcaacggc cggaaaagct ttgatttcga agagcttagg
     3061 gcgctgcggc cggctcgcgt cagtcctcgt tgacaagccg cttcaggcgg cgtttttcat
     3121 ttgctgtcaa tggcttaggt gattttgcga gcgccttgcg ccgccggtag ctgaacagaa
     3181 tgacgccgag cgccaccaac aacacaagcg gggtggcgaa ccacaaagcc caagtctggg
     3241 gcaacagacg cggcttcagc agtacgaact ccccatagcg ggacaccacg aaatcgatga
     3301 tctcctcgtc cgagtcgccg gccttcaagc gttcgcgcac caggacgcgc aaatccttgg
     3361 cgagcggcgc atccgaatcg tcgatcgact ggttctgaca aacgaggcac cgcaaccccg
     3421 ccgaaagcgc ccgggctcta ccttcgagcg caggatcgtc gagcacctcg tcgggttgga
     3481 ccgcgccggc cggtacggtc aagcaaagcc caacgacgag cgacagaaga aacgccaaga
     3541 gcggcccgcg tgtcgtcatg tcatcgctcc gcaggcactg cggcagactt cgcgcgcttg
     3601 cccgcctttt tgggcgcacc gacccgcaag cgccggtcgg acagcgagag ggcgccgccg
     3661 aaaaacatga tgagcgcgcc gatccagata aagcgcacga gcggattgaa atagaccctg
     3721 acactgtagg cgccgtcttt gagcggatcg ccaagcacca catagaggtc gccccgccaa
     3781 ccgttgtaaa tgcccgcctc gctggtcgcg ctcttctcca ccgtgaaggc gcgcttcgac
     3841 ggcgcgagct tggtgaccgg gcttccgccg cggctcacgg caaacacccc gatcctctct
     3901 tgatagttcg gaccttggtg cggggccacg ccttcgaatt tgagctgata gccggcgatg
     3961 tccaccgtgc cgccgggctg taacgcctcg atgcgctcac tgcgccacgc agtcgtcgcc
     4021 acgagaccaa gcaccatcac cccgagcccc gcatgcgcca gcgccgaacc gtaggctgcg
     4081 cgcggcaagt tccaagcacg acgccaactc tccgcgccag aggccttgaa cagcttcgtc
     4141 cggaacgcga tgtcggtcgc ggcgcccatc aaaagccaaa cgccaagacc gatgccgagc
     4201 ggcgccagcc acgggccctt ctggtagaac gcaagcgtaa gaataatggc cgcaagagac
     4261 acgatcgccg ccaccaagag ccgctgcgcg gcggccaaga gatcgccccg cttccaggcc
     4321 agcaatggac cgaacggtac ggcgatcagc agcggcacga tcagcggcac gaaggtcagg
     4381 ttaaagaacg gcgcgccgac ggagatcttc tcgcccgtta gcgcttcgag cgccaacggg
     4441 tagagcgttc cgaccagcac ggcggcgcac gccaccgtca gcagcaggtt gttgaggacg
     4501 aggctcccct cgcggctgat cggcgcgaaa atcccgcctt gcgacagcag cggcgctcgc
     4561 caggcgaaca gcgcgaaggc accgccaatg aataccgcca tgatgccgag gatgaacacg
     4621 ccgcgcgtgg gatcgacggc aaatgcatgc accgaggtga gcacgcccga ccgcaccaag
     4681 aaggtgccca tgaggctcaa ggagaacgtg atgatggcaa ggaggatggt ccacactttc
     4741 agcgcgtcgc gcttctccat caccaatgcc gaatgaagca gcgccgtgcc ggcgagccac
     4801 ggcatgaagg acgcattctc caccgggtcc cagaaccaga agccgcccca gccgagttcg
     4861 taataggccc accacgagcc aagagcgatg ccgagcgtca ggcacatcca ggcggcgagc
     4921 gtccagggcc gcacccagcg cgcccaggcc gcatcgatgc gcccgtcgat cagcgcggcg
     4981 accgcgaagg aaaaggccat agagaatccg acatagcccg catagagaaa cggcggatgg
     5041 atcgcgagcg cgggatcctg cagtacgggg ttgagcccct gcccgtcgaa cggcgccgga
     5101 ttgacgcgca cgaacgggtt ggacgtgatc acgataaaca ggaggaaggc gacagcgatc
     5161 gaaccctgca ctgcaagcac attggcttga agctggcgcg gtaaatttgt gccaaaggtc
     5221 gccaccgccg cgccgaacaa agcgaggatc agcacccaaa ggaccatgga accttcgtgg
     5281 ttcccccaca ctcccgagat cttgtagagc agcggcttcg ccgagtgcga attctgccag
     5341 acgttgagga cggaaaagtc cgacgtcacg tatgcgatcg tcagcgccat gaaggcaatt
     5401 acgatgagaa tgaactgaag ttgtgaggcc gggatcgcta cggcggtgag ttgggggtcg
     5461 cgaaagcggg tcccgatcac aggcaacacc gcctgcacga tggcaacgcc aagtgccagg
     5521 atcagtgcgt aatgtcccgt ttcggcgatc atcagttcac tgccccgccc gatttgctgc
     5581 cgccgtccgt tttggaatcg tcgcgccaaa caccctgctc tttcagcttc gcggcgacct
     5641 cgggcggcat gtagttctcg tcgtgctttg ccagcacact atcggcatgg aaaacgccgt
     5701 ccggctgcag cgtgccctcg gtcacaacgc cttgcccttc gcgaaacaga tccggcagca
     5761 cgcccgtata ggtgacgggc aaggtctttg cgccgtcggt gatggcgaag cgaaccgtcg
     5821 tgcctgcatc ccgcaccacg ctgccggttt ccacgagacc gcccagccgg aagcgctgtc
     5881 cgggtgtgac ctccatcttg gc
//
LOCUS       DBNHVU010000027         5683 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1497010, whole genome shotgun sequence.
ACCESSION   DBNHVU010000027 DBNHVU010000000
VERSION     DBNHVU010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5683)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1497010"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..160)
                     /locus_tag="AC16Y7_01050"
     tRNA            complement(84..160)
                     /locus_tag="AC16Y7_01050"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(124..126),aa:Met,seq:cat)
     gene            complement(210..1043)
                     /locus_tag="AC16Y7_01055"
     CDS             complement(210..1043)
                     /locus_tag="AC16Y7_01055"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5707997.1"
                     /translation="MESQRSVVDTDTVRHAYRRWAPIYDYTFGQVADAGRKHAVRIIN
                     KRKGRVLEVGVGTGLSLPGYGEQLTVTGIDLSPEMLEKAQEKVTKKKLGNVDGLHEMD
                     ASALAFADESFDTVVAMYVMTVVPDPDKVMRELERVCAPGGEVILVNHFSQEEGVRGF
                     LEKKLAPFADLIGWHAVFEVDRVLICNDLRLVERRSLWPIGLFTMLRFVKEPGFGVAP
                     QAARYMLAKGRREAPARAPEPIRVRAMRMTARGGVFARRLLDTNYWRSLYRRLQSQLT
                     E"
     gene            complement(1086..2294)
                     /gene="mnmA"
                     /locus_tag="AC16Y7_01060"
     CDS             complement(1086..2294)
                     /gene="mnmA"
                     /locus_tag="AC16Y7_01060"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041799471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR5707998.1"
                     /translation="MLNSLHLPGRPADHRVVVAMSGGVDSSVVAALLKAQGYDVVGIT
                     LQLYDHGQAVGRKGTCCAGQDIQDARRVAETLQIPHYVLDYEARFAQAVMGEFADSYL
                     NGETPVPCVACNQKIKFHDLLQTAEGLGAAALATGHYVRSMPGASGWELHRAEDKDRD
                     QSYFLFNTTPEQLAFLRFPLGDLPKEETRKLARDYGLAVAEKSDSQDICFVPTGRYTQ
                     VIERLRPGAAEPGDIVHVDGRVLGRHTGIINYTIGQRKGIGVAASEPLYVVKLDANRR
                     EVVVGPRDALRAQTLLLRDVNWLGDEPFDAAAAEGIEVLARIRSTGPLQRATVRLDDD
                     SVKISLADGEEGVSPGQACVFYAAMGGGERLLGGGWIKSAEDGAWLAKDGFAAQASPL
                     PVEAFLAGSR"
     gene            2640..2918
                     /gene="sciP"
                     /locus_tag="AC16Y7_01065"
     CDS             2640..2918
                     /gene="sciP"
                     /locus_tag="AC16Y7_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CtrA inhibitor SciP"
                     /protein_id="HIR5707999.1"
                     /translation="MTEHYRPRVRYVIGPDGSPLTVADLPPKNTKRWVIRRKAEVVAA
                     VRGGLLSLEEACERYTLTVDEFLSWQRSIDKHGLPGLRATRIQDYRSG"
     gene            complement(3009..3521)
                     /locus_tag="AC16Y7_01070"
     CDS             complement(3009..3521)
                     /locus_tag="AC16Y7_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708000.1"
                     /translation="MFRLGATMLALGLAALWVTACGGRSTGSASVAESTANVAATEAV
                     GTVPAYNTTRMSIATAKPVDAYVLLGGRIKSCWFNPSAPLFPEHVYRADVSPDGQKVK
                     ITIHDKRNLGRAGKLAYSIDFAQQGSQTAVTSRNLTMTPQQAAKMQFDVNRWKSGQKD
                     CSKKWPKVAG"
     gene            complement(3552..4037)
                     /locus_tag="AC16Y7_01075"
     CDS             complement(3552..4037)
                     /locus_tag="AC16Y7_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HIR5708001.1"
                     /translation="MPSFDIVSKTDLEEVDNALANMVREMSQRYDFKGSQCKIERKEA
                     EITINADDDLKLKQMHELLQGHLARRKIEAGAIDYQTAEKAAGQSMRQKAIIRQGIDK
                     ELAKRIVKSVKDAKFKVQVAIQGSELRVTGKKRDDLQAVIEHIKGLPLELPLQYVNFR
                     D"
     gene            complement(4160..4561)
                     /locus_tag="AC16Y7_01080"
     CDS             complement(4160..4561)
                     /locus_tag="AC16Y7_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HIR5708002.1"
                     /translation="MKSRDAVIRLKRFEVEEKCRKVAEIESMIGEFNTMVSDLDRQIA
                     IEQERAGVSDVNHYAYPTFAKAAIQRRDNLSNSVAGLDAKLAAARAELDEAVEELKKI
                     ELLQARDSDRVRVETPAPADLVAVGAHRAAS"
     gene            4937..5632
                     /gene="ctrA"
                     /locus_tag="AC16Y7_01085"
     CDS             4937..5632
                     /gene="ctrA"
                     /locus_tag="AC16Y7_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor CtrA"
                     /protein_id="HIR5708003.1"
                     /translation="MRVLLIEDDSATAQSIELMLQSEGFNVYKTDLGEEGVDLGKIYD
                     YDIILLDINLPDMSGYEVLKTLRVSKVQTPILILSGLAGIEDKVRGLGFGADDYMTKP
                     FHKDELVARIHAIVRRSKGHAQSVIQTGGLKVNLDTKTVDVNGQRVHLTGKEYQMLEL
                     LSLRKGTTLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLAAATGGKHYIETVWGRG
                     YVLRDPEEQAEAA"
BASE COUNT         1096 a         1824 c         1654 g         1109 t
ORIGIN      
        1 atgtatgtag ggtatgaaac ccaattgcta catacaagta gtttggtcgt tggcatatat
       61 ccgattgttt ttattgatct ttttggtagc ggaggaggga ctcgaacccc cgacacgcgg
      121 attatgattc cgctgctcta accagctgag ctactccgcc ccaaggccaa acgccctgtc
      181 atctatatag gtcaggggct gccgcgccgc tattcggtca attggctttg aagtcggcga
      241 tatagagacc gccagtaatt ggtgtcaagc aaacgccgcg caaagacgcc gccgcgcgcc
      301 gtcatacgca tggcgcggac ccggatgggc tcaggcgccc tcgccggcgc ttcccgccgg
      361 cccttggcca gcatgtaccg cgcggcttgc ggcgccaccc cgaaccccgg ctctttcacg
      421 aagcggagca tggtgaaaag gccgatgggc cacagcgacc ggcgctccac gaggcgaaga
      481 tcattgcaga tcaacacgcg gtcgacctca aacaccgcat gccaaccgat gagatcggca
      541 aaaggcgcca gcttcttctc caggaatccg cggacgccct cttcctggct gaaatgattc
      601 accaaaatca cctcgccgcc cggcgcgcag acccgctcca gctcccgcat caccttgtcg
      661 ggatcaggca ccacggtcat gacgtacatc gccaccacgg tgtcgaagct ctcatcggca
      721 aaggcgagcg cgctcgcgtc catctcgtgc aagccgtcga cattgccgag cttcttcttg
      781 gtgactttct cctgcgcctt ctccagcatc tccggcgaaa ggtcgatgcc cgtgaccgtc
      841 agttgctcgc catagcccgg cagcgacagg ccggtgccga cacctacttc gagcacgcgc
      901 cctttccgct tgttgatgat ccggaccgca tgcttccggc cggcgtccgc cacctgaccg
      961 aacgtatagt cgtagatggg cgcccagcgg cgataagcat ggcgcacggt gtccgtgtcc
     1021 actacggaac gttgtgactc cactgtggga tcctccccga ctacaggccg cctattcgcc
     1081 gcggcctaac gcgacccggc caagaaagcc tctaccggca atggcgatgc ctgtgccgca
     1141 aaaccgtcct tggccaacca ggctccgtct tcggcagact tgatccatcc gccgccgaga
     1201 agccgctccc cgccgcccat cgcggcataa aatacacagg cctgtcccgg ggacacgccc
     1261 tcctcgccgt cggcgaggct gatcttgaca ctatcatcgt ccaggcgaac cgtcgcgcgc
     1321 tgcagcggac ccgtggagcg gatccgcgcc agcacttcga tgccctccgc cgccgccgcg
     1381 tcgaacggct cgtcaccaag ccagttcacg tcgcgcaaga gaagcgtctg ggcacgcaag
     1441 gcatcgcgcg gaccgacgac aacctcgcgg cggtttgcgt cgagcttcac gacgtagagc
     1501 ggctcgctcg ccgccacgcc aatgcccttc cgctggccaa tcgtataatt gatgatgccg
     1561 gtgtggcggc caagcacgcg cccgtccaca tgaacgatgt cgccgggctc cgcagcaccg
     1621 ggacgcagac gctcgatcac ttgcgtgtaa cgccccgtgg gcacgaagca gatatcctgg
     1681 ctgtccgact tctcggcgac cgcgagccca tagtcgcgtg ccagcttgcg cgtctcttcc
     1741 ttcggcaggt ccccaagcgg aaaccgcaaa aaggcaagct gctccggggt cgtattgaat
     1801 agaaagtagc tctggtcgcg atccttgtcc tcggcccggt ggagctccca cccggacgcg
     1861 cccggcatgc tcctcacgta gtgcccggtt gccaacgccg cggcgccgag gccctcggca
     1921 gtctgaagca aatcgtggaa cttgatcttc tggttgcacg ccacgcacgg caccggcgtc
     1981 tcgccgttga gatagctgtc ggcgaactcg cccatgacgg cctgcgcgaa gcgcgcctcg
     2041 taatcgagga cgtagtgcgg aatctgaagc gtctccgcga cccgccgcgc atcctgaatg
     2101 tcctggcccg cgcaacacgt gcctttgcgc ccaacggcct ggccgtggtc gtaaagctgc
     2161 agcgtgatcc cgaccacgtc gtagccttgg gccttcagca gggccgcaac cacggaggaa
     2221 tcgaccccgc ccgacatggc gacaaccacg cggtgatcgg ccgggcggcc aggaaggtgc
     2281 aaactgttaa gcattctcgg cttcaataat aatggcgatc caggagttct cgaaggatcc
     2341 tgacggcaaa tgatcttgcc cgtcccacag tataaggggc catatcggac ccgtgtggcc
     2401 ttcgaggcaa cccctacccg gagcagaaca tagggagcga ccccttggga agcaaggcta
     2461 ttggcgcctc cggtgcctgc cagggcgact tcgaggggag cggaagtttc agtgaattca
     2521 gcaagttttg gctatttcct tgccgcttct tttaccccac agcttaggca attcttaaat
     2581 ggggctctgc tagcgtccgc ctagatttaa gatcatcgat tttgtgagtg ttgcgtacga
     2641 tgactgaaca ttacaggccg cgtgtcagat atgtgattgg accggacggc agcccgctga
     2701 ccgtcgccga cctgccaccc aagaatacca agcggtgggt catccgccgg aaggccgagg
     2761 ttgtggccgc cgtgcgtggt ggtctgctga gcctcgagga ggcctgtgag cgctatacgc
     2821 tcaccgtgga cgagttcctg agctggcagc gctccatcga taagcacggc ctgcctggcc
     2881 tgcgcgcgac ccgcattcag gactatcgca gcggctaacg gcccgccgat acatcttctg
     2941 agatttcctt gatggccggg tcccaagccc ggccatttgc acgtccgggc tcgctagcgc
     3001 gccgcaggct agcctgcgac cttcggccat ttcttgctgc aatccttctg tccgctcttc
     3061 cagcggttga cgtcgaactg catctttgcc gcctgctgcg gcgtcatggt gaggttccgc
     3121 gaggtcaccg ccgtttggct gccttgctgg gcaaaatcga tcgaataggc gagcttaccg
     3181 gcccgaccaa gattgcgctt gtcgtggatg gtgatcttca ccttctggcc gtcggggctc
     3241 acatcggcac ggtagacatg ctcggggaaa agcggggcgc tggggttgaa ccagcagctc
     3301 ttgatccgcc cgccgagcag tacataggca tcgacaggct tggccgtcgc gatcgacatg
     3361 cgggtcgtgt tgtaggcggg cacggtaccg acggcctcgg tcgcggcaac gttcgcggtg
     3421 gactcagcca cgctggcact gcccgtggac cgaccgccac aggcggtcac ccaaagggcc
     3481 gcgagcccaa gcgccagcat cgtcgcgccc aacctgaaca cgctatgtcc cttcaagaga
     3541 atcaccagcg gtcagtctct gaagttcaca tattggagcg gcagctcaag cggaagccct
     3601 ttaatgtgct cgatcacggc ctgtagatcg tcccgcttct tgcccgtgac ccgcaattcg
     3661 ctgccttgga tggcgacctg gaccttgaat ttggcgtcct tgacgctctt gacgatgcgc
     3721 ttggccagct ccttgtcgat gccttggcgg atgatcgcct tttggcgcat ggactggcct
     3781 gcggccttct ccgctgtctg ataatcgata gcccctgcct cgatcttgcg gcgcgccaga
     3841 tggccctgga ggagctcgtg catctgcttg agcttcaggt catcgtcggc gttgatggtg
     3901 atctccgcct ccttgcgctc gattttgcac tgcgacccct tgaagtcata gcgctggctc
     3961 atctcacgca ccatgttggc gagagcgtta tcgacctctt cgaggtcggt cttggatacg
     4021 atatcgaatg acggcatgga cccttcccgc gctcacagac tgacctggat actagccacc
     4081 tgagacgccg gacgcgagcg gtccccgtgg gcagcaagcc acgggccact cgacaggcct
     4141 caggaccgct gaaagtcggc tagctggctg cgcggtgagc gcccaccgcc acgagatccg
     4201 caggcgcggg ggtctcaaca cggacccgat cgctgtcgcg cgcctgaagg agctcgatct
     4261 tctttagctc ctcaacggcc tcgtccagct ccgcgcgcgc ggccgcaagc ttggcatcca
     4321 ggccggccac ggaattcgac aggttatccc gccgctggat cgccgccttg gcgaaggttg
     4381 gataggcata atgattgacg tcggagacgc cggcccgctc ttgctcaatg gcaatctgcc
     4441 gatcgaggtc ggaaaccatg gtgttgaact cgccgatcat ggattcgatt tcggcgactt
     4501 ttcggcactt ctcctcgact tcgaagcgct tcaaccggat gactgcgtcg cgtgacttca
     4561 taactcaata ccctcagcct gctcgggccc cgctcacgga gatacaatta ccatgccagc
     4621 ggcgcgctcc ggctgagcca ttgcccaaag aacaatgcga cccccaaagc gaacctaggc
     4681 cttctttcga ggactggtcc ccctgcccca ctctccagat catggccgac aggccttaag
     4741 ggccggtaaa ccatatgggt tgcatttaaa ggatgggcca ccccgcctga ggtagggatg
     4801 gttagcgagt gtggttagca ggcttagctt ttgttaacta ttggccacta atttattgat
     4861 tcgaattaac caatctgcgt ccgacgtact gggtcagggg ataaccagga cattgagaat
     4921 acgggggaat taaaaaatgc gggttcttct gatcgaggac gatagcgcca cggctcagag
     4981 catcgaacta atgctccagt cggaaggctt taacgtctac aagaccgatc taggcgaaga
     5041 aggcgtcgat cttggtaaga tctacgacta cgacatcatt ctgctcgaca taaaccttcc
     5101 ggatatgagc ggttacgagg tcttgaaaac cttgcgggtc agcaaggtac agaccccgat
     5161 cctgatcctc tccggactgg ctgggatcga ggacaaggtc cggggcctgg gcttcggggc
     5221 cgacgactac atgaccaagc ccttccataa ggacgagctc gttgcccgca tccacgcgat
     5281 cgtgcggcgc tcgaagggcc acgcccagtc cgtcatccag acaggcggcc tgaaggtcaa
     5341 tctggacacc aagacggtcg acgtgaacgg ccagcgggtc cacctgaccg gcaaggaata
     5401 tcagatgctt gagctgctct ctctgcgtaa gggcacgacc ctgaccaaag agatgttcct
     5461 gaaccacctc tatggcggca tggacgagcc cgagctcaag atcatcgacg tcttcatctg
     5521 caagctgcgc aagaagctcg ctgcggcgac tggcggcaag cactacatcg agacggtctg
     5581 gggccgcggc tacgtgctgc gcgatcccga ggagcaggca gaagctgcct gattgatttt
     5641 cactgcgaga gaccgaacgg cccgccctaa ccggcgggcc gtt
//
LOCUS       DBNHVU010000028         3569 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_11964, whole genome shotgun sequence.
ACCESSION   DBNHVU010000028 DBNHVU010000000
VERSION     DBNHVU010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_11964"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            856..3159
                     /locus_tag="AC16Y7_01090"
     CDS             856..3159
                     /locus_tag="AC16Y7_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR5708004.1"
                     /translation="MISSAAETAAQDAPSETGTQEAQEFVEVYELPPIEVTVDSPVKK
                     TSAPKRPSGAASRPSPAPSRTTSTRSAAAVGDAPDRVLDLDIPAGVAVVTGDSFVPVT
                     VATEREILANGGATITDSLQNKPGIAGSTFAPGANRPIIRGLDSYRIRIQENGIGTHD
                     VSAISEDHAIPIDPSDADRIEVVRGPSTLRYGSQAIGGVVSVENQRVPTYIPDRGFRS
                     VVKGGGSTVDDGGDGAFSVTGGAHGIVLHADGFIRDVNDYDTPLGSELNTFVKSRGGS
                     AGISHVWRNGFIGVSYSRFESLYGIPGEEAEEGVLPRIDLEQDKVLVKGEWRPDSSGI
                     EAVRVWFGASDYAHNELARHGEHGHGEEEHEEEGEGHEEEEHEEEEVAGFSVGQTFTN
                     REQEARLEIQHRPVASRFGPLSGAIGVQWDHANTKGIAFEADSLLEPYNTESIAGFWF
                     EELEANARLRLQLALRVEEASVDGTGFSSIADPEAPVVFSGTRSFVPLSGGLGALYDL
                     GNNTVLRLNGQAVERAPAGPELFSKGIHEATGTFEIGNPFLDEEKAQTLELAVKRATG
                     RFRYDANGYYTNYDGFIFRELTGLVCGGTLDSCAAALDEEETFDQVLFQQRNATFYGV
                     ELLGEYDVGRVWNGIWGIDGQYDFVRARFDNGENVPRIPPHRLGGGIYYRDGKWFART
                     AVLHAFDQDQVAPGELTTPSYTLLNAELSYALNTAGPAGLTPAMNIGLKGENLLDDKV
                     LNSASFKRREGVLEPGANVRIFGKISF"
BASE COUNT          718 a         1052 c         1105 g          694 t
ORIGIN      
        1 gatgtccgct ttgggtcatt agccgacatc agagcgcgga tcagggatgt cggctttgtc
       61 cctacaagcg gacattgcca gcgtcggtgt cgaggtcagc taagtgccat aagcggtcat
      121 gaaccgcttt cgtcactagt gttgtattat agcaaccatg gatcttgatg tgcctgttat
      181 gttgtaacaa tgcgttactt tcggttttcc cgtcatggac tgtcgtggct cgcactgatc
      241 gcatttgcgg gacagaccat cctgaccttg ggtcatgttc atggcctcga tcacgagatc
      301 gcaagagcgc aaatcgagga gatggatgaa ctgccctcgc attatcgcga ggctccggcc
      361 gatgccgaac accgtgaaac accggaggat tcggatgatt tagacctctc gtgcgctgtt
      421 tgtcggatga tggcgcaggc ggagtcctca attatcccta gcccactacc gatccggatc
      481 aagcgcactc aaacaagctt tcttcccatc ttttcagcag gcgcgttctc aacagcagag
      541 aacgcctccc cattcgcgtc gcgcgcaccc ccactaacat caacttagat ggcctgacgg
      601 cccatttgca tcgtcactcc tgcgcaccag gatcgtcact cctgcgcacc aggtaagtgc
      661 gctgtgcgga aacgagcgtg ggccggagtt agtcggcata agtttgtgat cgaggtgggt
      721 atgtggacgg atttcagaag gcgtatttgt cttttagtga catttgaatg ttacattgta
      781 acagacaagt tgtgcaataa tataacagtt tggtttggca ggggcgcagc tggtctctgc
      841 tcgattttcg ttctcttgat ctccagtgcg gctgaaaccg ccgcgcaaga tgcaccgagc
      901 gagacgggca cgcaggaagc gcaggagttc gtggaagttt acgaacttcc gccgatcgaa
      961 gtgaccgtcg atagtccggt caagaagacc tcggcgccca agaggccaag cggggcggcg
     1021 tcccggccga gccccgcgcc ttcacgcacg accagcacgc gaagcgcggc agcggtcgga
     1081 gacgcacccg acagagtgct cgacctcgac attccggccg gtgtcgccgt ggtcactggc
     1141 gatagtttcg tgcccgtcac cgtggcgacg gaacgcgaga tcttggccaa tggcggcgcg
     1201 accatcaccg acagcctgca gaacaagccc ggcatcgcgg gatcaacgtt tgcgcctggc
     1261 gccaaccggc ctatcattcg cggcctcgac agctaccgga tccgcattca ggagaacggc
     1321 atcgggaccc acgacgtctc ggctatctcg gaggatcacg ctattccgat cgatccgtcc
     1381 gacgcggacc gcatcgaagt cgttcgcggt ccatcgacgc tgcgttacgg ctctcaagcg
     1441 atcggtgggg tggtctcggt cgagaaccag cgcgttccca cgtacatacc ggatcgcggc
     1501 tttcgcagcg tcgtcaaggg cggcggcagc acggtcgatg acggcggcga cggtgctttc
     1561 agcgtcacgg gcggcgcgca cgggatcgtg ctgcatgccg acgggttcat ccgcgacgtc
     1621 aacgactacg acaccccgct cgggtcggag ctcaacacct tcgtgaagag ccgcggcggg
     1681 tcggccggca tctcccatgt ctggcgcaac ggcttcattg gcgtctctta ctcgcggttt
     1741 gaaagtctgt atggcattcc cggcgaggag gccgaggagg gcgtgctgcc gcgcatcgac
     1801 ctcgagcagg acaaggttct cgtcaagggc gagtggcgtc cagattcctc tggcattgaa
     1861 gcggtgcgcg tctggtttgg tgcctccgac tatgcgcata acgagctcgc caggcacggc
     1921 gaacacggac atggcgagga agaacatgag gaagagggtg aggggcacga agaggaagag
     1981 catgaggaag aggaggtcgc cggcttctcc gtcggccaaa ccttcaccaa cagggagcag
     2041 gaagcgcggc tggagatcca gcaccgcccc gtcgccagcc gcttcggccc cttgagtggc
     2101 gccatcggcg tccaatggga tcacgccaac acgaaaggca tcgcctttga agctgactcg
     2161 cttctggagc cttacaatac ggagagcatt gccggttttt ggttcgaaga gcttgaggcc
     2221 aatgcgcggc tccggcttca gcttgccttg cgggtcgagg aagcgtcggt ggacggaacc
     2281 ggcttctcca gcatcgccga tccggaagcg cccgtcgtgt tcagcggcac gcggtccttc
     2341 gtgcccttga gcggcggcct cggcgcactc tacgatcttg gcaacaacac ggtgctgcgc
     2401 ctcaatggtc aggccgtcga gcgagcgcca gcaggtcctg agctcttctc gaaggggatt
     2461 cacgaagcga ccggcacgtt cgagatcggc aacccctttc tcgacgaaga gaaggcccag
     2521 acgctggaat tggcggtgaa gcgcgcgaca ggaaggttcc gctacgacgc gaacggctac
     2581 tatacgaact atgacggctt catctttcgc gagctcaccg ggctcgtttg cggtggcacc
     2641 ttggattcct gtgcggcggc tctcgacgaa gaggagacgt tcgaccaagt gctgttccag
     2701 cagcgaaacg ccacattcta cggcgtcgag ctactcggcg agtacgacgt cgggcgggtc
     2761 tggaacggca tctggggcat agacggccag tacgatttcg tccgcgcgcg cttcgacaac
     2821 ggcgaaaacg tgccgcgcat tccgccccac cgcctcggcg gtggcatcta ctaccgggac
     2881 ggcaagtggt tcgcgcgcac cgccgtgctg catgccttcg atcaggacca ggtggcgccg
     2941 ggcgagctga ctacgccgag ctacacgctg ctcaacgcgg agctgagcta cgcgttaaac
     3001 acggcgggcc cggccggcct gacgcccgcc atgaacatcg gcttgaaagg cgagaacttg
     3061 ctggacgaca aggtgctgaa ttcggcctcg ttcaagcgcc gggagggcgt gctggagccg
     3121 ggcgcaaatg ttcgcatatt tgggaaaata agcttttgac ggcgtgtcac cggtgaatcg
     3181 tcgaacgcgc cgatcagtat ttctcgaggc taggccaaac accgtttaca tcagcgtctg
     3241 ggacacgggt atcgaaccgc agcctcaaca gctcgtgaag cccggtgcct ggcacgatac
     3301 tgtcgccaag cggatcgcct cgaaggactt gtcacccaag tagggccctg aacctaaaca
     3361 aggaaggcga gaccaaggtg ccggtggcaa ggacggcatc cgcgtcacat ctggtgaacg
     3421 ttactgaatg tccgctttgg gtcattagcg gaaatcagaa cgcgacggag agacggccgc
     3481 tttacaccca taagcggaca tcctcggcgc ggattttggc ttttgattgc cttcagtcat
     3541 tgcagcaatg ccctttcggc cgattccaa
//
LOCUS       DBNHVU010000029         6886 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1497474, whole genome shotgun sequence.
ACCESSION   DBNHVU010000029 DBNHVU010000000
VERSION     DBNHVU010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6886)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6886)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6886
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1497474"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="AC16Y7_01095"
     CDS             complement(<1..1059)
                     /locus_tag="AC16Y7_01095"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR5708005.1"
                     /translation="MNDDVEMEGLPRPLAAARAIDPFIVMDVMRDANAREVAGEDIIH
                     MEVGQPATAAPRAARERAAAALRDDRLGYTEALGLPALRERIAAHVKERYGVRVGPER
                     IVVTSGSSAGFVLAFLAILDHGAGLGLPSPGYPCYRQILKALGMRPHLIETSGQGRWM
                     PTAADVDRLGTDGASGLLLASPNNPTGTMAMGACLAEIADACRRARLWLISDEIYHGL
                     EYGAPAETALSHSDAAIIVNSFSKYFSMTGWRIGWLVVPEALTRALERLAQNLYISPP
                     AISQIAALGAFDGIDELEEIKARYARNRAMLLEELPRAGLDAILPADGAFYLYADISR
                     YTGDSAAFARDMLREIGVA"
     gene            1308..2669
                     /locus_tag="AC16Y7_01100"
     CDS             1308..2669
                     /locus_tag="AC16Y7_01100"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR5708006.1"
                     /translation="MLVAALIGSVICSTAQARGIIRDAETEALIRDYAKPIFRAAGVG
                     SQNIRIHIVNDRNFNAFVVDGHNMFMHAGALMIAETPNQVIGVIAHETGHITGGHLAR
                     LRSQMARAKSASLMLQLLGLAAVAGGAIAGIPNAGQLGMGAAMGGQQAALRSLLAYRQ
                     TEESSADQAAMSFLNATKQSGRGMIETFDVLAEKLRGVQGINPYLMSHPLPQTRIAQL
                     RQLVEQSPYYDKKDSPELQFRHDLIRAKLSGFLDKPEIAFNRYPSSDTTLPSIYARAI
                     ATYRQSGVDAAMPLLNKLIAEQPNWPYFYETKGQFLFESGRGAEAIAPLKKAIELAPH
                     EAIVQIMYAQALIGTEDNRYLDEAIRNLRQALVRETSSARGYRQLATAYARKAHLAKA
                     SGAKRNFMARASLASAEAYFYEGRLDRAKQQAKRAKAGLPEGSPNWIRADDILSFQLP
                     KKK"
     gene            2697..3500
                     /locus_tag="AC16Y7_01105"
     CDS             2697..3500
                     /locus_tag="AC16Y7_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR5708007.1"
                     /translation="MTSKTGLATALVAAVVAAIVSAATLLIVGRTAPGVATAPTGGLD
                     EKQVIEVVRNYLTENPEILVEMTTKLDKIQQEKQAADQVSAISENSDALFRSTKAFGA
                     GNPDGDVTVVEFFDYNCGFCRRAMPEVVKLVDNDDKVKVVFMELPIFGDESEDAAKGA
                     LAAGKQDKYMEMHQKLFTAPGKANKEKVLRIANEIGLDVPQLEKDMEGDEVQAALDEA
                     RGLAQALGLQGTPLYLVGDRIIPGAPDDLYDQLVEKVTEVREKGCTATC"
     gene            3648..4100
                     /gene="aroQ"
                     /locus_tag="AC16Y7_01110"
     CDS             3648..4100
                     /gene="aroQ"
                     /locus_tag="AC16Y7_01110"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR5708008.1"
                     /translation="MAQTIQILNGPNLNLLGTREPEIYGSETLDDLHARCERKAKALG
                     YEVVFRQSNHEGELVSWVQEARERAAGLIVNAGAFTHTSVALLDALLSCSVPIVEVHL
                     SNIFRREPFRHRSYVSKAAKGVICGFGGLGYELAIEALATSLAEAKAG"
     gene            4120..4599
                     /gene="accB"
                     /locus_tag="AC16Y7_01115"
     CDS             4120..4599
                     /gene="accB"
                     /locus_tag="AC16Y7_01115"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008194390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR5708009.1"
                     /translation="MNKDGGNLDKDLIRELAELLEETGLTEIEIELKSGRFRVARGVN
                     LTTTLMTTGNGAPPPAPPQEHREPKRASETEDVAVHPGSVTSPMVGTAYRSAEPGAPP
                     FVEVGSRVSAGQTLLIIEAMKTMNHIPSPKAGTVLAILVEDGQPVEYGEPLVVIEQG"
     gene            4609..5964
                     /gene="accC"
                     /locus_tag="AC16Y7_01120"
     CDS             4609..5964
                     /gene="accC"
                     /locus_tag="AC16Y7_01120"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR5708010.1"
                     /translation="MFDKILIANRGEIALRIERACKELGISTVAVHSTADAHAMHVRL
                     ADESVCIGAPPAKDSYLNIPAIVAACEITGADAVHPGYGFLAENARFAEILDEHNIAF
                     IGPTAEHVRLMGDKIKAKETAKSLGIPVVPGSDGPVTNYDEAKKIAAKIGFPVLVKAS
                     AGGGGRGMKLAHFENELEPALATARNEARAAFGDDTVYLEKYLGQPRHIEVQVLGDGE
                     GNAIHLGERDCSLQRRNQKLWEEAPSPALNDNERLRIGEIVADAMRKLKYRGVGTVEF
                     LYENGEFYFIEMNTRLQVEHTVTEMVTGLDLVIEQIRIAAGAPLTLAQEDVSFIGHAI
                     ECRVNAENALTFRPSPGTITHFHPPGGLGVRVDSGAYAGYTIPPYYDSLIGKLIVHAR
                     TRNECLMRLKRALGEFVVDGIETTIPLFSSLISEPDIVDGSYDIHWLEDHLAPNTERP
                     G"
     gene            5971..6639
                     /gene="aat"
                     /locus_tag="AC16Y7_01125"
                     /pseudo
     CDS             5971..6639
                     /gene="aat"
                     /locus_tag="AC16Y7_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087059.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
     gene            complement(6537..6839)
                     /locus_tag="AC16Y7_01130"
     CDS             complement(6537..6839)
                     /locus_tag="AC16Y7_01130"
                     /inference="COORDINATES: protein motif:HMM:NF021444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HIR5708011.1"
                     /translation="MFAESPGLHAVEHPVFDVWLTSCKMPIGGAPSESAENAPSEPGA
                     EGEAMAEPAPAPAAPAPPPAAAPAPPPAAAAPRPRPAPPPGPGGLPWLPNAPSRSP"
BASE COUNT         1362 a         2210 c         2157 g         1157 t
ORIGIN      
        1 ggccacgccg atctcgcgca gcatgtcacg cgcgaaggcg gcgctgtcgc cggtgtaacg
       61 gctgatatcc gcatagagat agaacgcacc gtcagcgggc aggatggcgt cgaggcctgc
      121 gcgcggcaat tcctccaaga gcatggcgcg gttgcgcgca tagcgggcct tgatctcctc
      181 aagttcgtcg atgccatcga aggcgccgag cgcagcaatt tgcgaaatgg ccggcggcga
      241 gatgtagaga ttctgtgcca gccgttcgag cgcgcgggtg agcgcctccg gcaccacgag
      301 ccagccgatg cgccagcccg tcatgctgaa atatttcgag aagctgttga cgatgatggc
      361 ggcgtcgcta tgcgacagag ctgtctcggc cggcgcgccg tattcgagcc cgtgatagat
      421 ctcgtcggaa atcagccaca gccgagcgcg gcggcacgca tcggcgatct ccgccaagca
      481 cgcacccatc gccatagtgc cggtcgggtt gtttgggctc gccagtagga gcccggacgc
      541 gccgtccgtg ccaagccggt ccacatcggc cgcggtcggc atccaacgcc cctgcccgga
      601 cgtttcgatc agatgcggac gcatgccgag cgccttcagg atctgccggt agcacgggta
      661 accgggcgac ggcaggccaa gcccggcacc gtgatcgagg atggcgagaa aggccagcac
      721 gaagcccgcg gacgatcccg acgtgaccac gatgcgctcc ggcccgacgc gaacgccgta
      781 gcgctccttc acatgcgccg cgatgcgctc ccgcaatgcg ggcagcccca aagcctcggt
      841 atagcccaac cggtcgtcgc gaagcgcggc cgcggcccgt tcgcgtgcgg cacgcggcgc
      901 ggccgtggcg ggctggccca cctccatatg gatgatgtcc tcgccggcca cttcgcgcgc
      961 gttggcatcg cgcatcacgt ccatgacgat gaaaggatcg atggcccgcg ccgcagccaa
     1021 tggcctgggc agcccttcca tttcgacgtc atcgttcacg gagcgaacct cgcgcttaag
     1081 tgatcgcaac cgtttcgggc ccgtcacgtt gtcgcctcat tcaaaagtca agcaaatcaa
     1141 agcttggcaa cggcgggggc cggaagcggc gtgccaacct aacgccctaa gaaggcaaaa
     1201 caaaataggc gtctttgccg ctgcccacta agcgaatacg atcacgtccg accatgcctt
     1261 gtctggctca ttcccgctct cgtcgaacgc cgtcgcgcct gcgcacggtg cttgtcgctg
     1321 cgctcatcgg tagcgtgatc tgctcaacgg cgcaggctcg cggcattatt cgcgatgccg
     1381 agactgaggc gctgatccgc gactatgcca agccgatctt ccgcgccgcg ggcgtcgggt
     1441 cgcagaacat tcgcatccac atcgtcaacg accgtaactt caatgccttc gtggtggatg
     1501 ggcacaacat gttcatgcat gccggcgcgt tgatgatcgc cgaaacgccg aaccaggtga
     1561 tcggtgtcat cgcgcacgaa accgggcaca tcaccggcgg ccatctcgcg cggttgcgct
     1621 cgcagatggc cagggcgaag tcggcctcgc tcatgttgca acttctcggc ctcgcggccg
     1681 tggcgggcgg cgcgatcgcc ggcatcccga acgcgggtca gctcggcatg ggggcggcca
     1741 tgggcggcca gcaagccgcc ttgcgcagtc tgctcgctta ccggcagacc gaggaatcgt
     1801 ccgccgacca ggcggcgatg agcttcctca atgccaccaa gcaatcgggg cgcggcatga
     1861 tcgagacctt cgacgtcttg gccgagaagc tccgcggcgt tcaaggcatc aacccgtacc
     1921 tgatgtctca ccccttaccc caaacgcgta tcgcacagct gcggcaactc gtggagcaga
     1981 gcccctacta cgacaaaaag gactcgcccg agcttcagtt ccgccacgac ctgatccggg
     2041 cgaagttgtc gggctttttg gataagccgg agatcgcgtt caaccgctat ccgtcgagcg
     2101 acacgacgct tccgtctatt tatgcgcgcg caatcgcgac ctaccggcag tccggcgtgg
     2161 acgccgccat gccgctgctc aacaagctga tcgccgaaca acccaactgg ccctatttct
     2221 atgagaccaa agggcagttc ctgttcgaga gtggccgggg ggcggaggcg attgctcccc
     2281 tcaagaaagc cattgagctt gcaccgcacg aagcgatcgt tcagatcatg tacgcgcaag
     2341 ccttgatcgg gaccgaagac aaccgctatc tcgacgaagc cattcgcaat ctgcgccaag
     2401 cgctggtccg cgaaacctca tccgcgagag gctaccggca gcttgcgacg gcctatgcgc
     2461 gcaaggccca tctggccaag gcgagcgggg caaagcgcaa tttcatggcg cgcgcatcac
     2521 ttgcgtcggc ggaagcctat ttctatgaag gtcgtttaga cagggcgaaa caacaggcca
     2581 agcgggccaa ggccggactt cctgagggct cgccgaactg gatcagagcc gacgacattc
     2641 tttcctttca attgccgaag aagaaataac tcgaaccaaa ccctggagag ctaagcatga
     2701 cctctaagac aggactcgct accgcgctgg tggccgccgt cgtagccgcc atcgtgagcg
     2761 ctgcgacgct cctcattgtc gggcgcacgg cgccaggggt cgccaccgcc cccacaggcg
     2821 gactggatga gaagcaggtc atcgaggtgg tgcgtaacta cctcaccgag aaccccgaga
     2881 ttctcgtcga gatgacaacg aaactcgaca aaattcagca ggagaagcaa gccgccgacc
     2941 aggtgtcggc tattagcgag aattccgacg cgctgttccg gtccaccaaa gccttcggcg
     3001 ccggcaatcc ggacggcgac gtcaccgtgg tcgagttctt cgattacaat tgcggtttct
     3061 gccgccgcgc catgcccgag gtcgtcaagc tcgtcgacaa cgacgacaag gtgaaggtgg
     3121 tcttcatgga gttgccgatc tttggcgatg agtccgaaga cgccgccaaa ggcgctctcg
     3181 ccgcgggcaa gcaggacaaa tacatggaga tgcaccagaa gctgttcacg gcgcccggca
     3241 aggccaacaa ggaaaaggtg ctgcgcatcg ccaacgagat cgggctcgac gttccgcagc
     3301 tggagaaaga catggagggc gacgaggtcc aagccgctct cgacgaggcc cggggcctcg
     3361 cccaagcact cggcctgcag gggacgccgc tctacctggt cggcgaccgg atcatccccg
     3421 gcgcgcctga cgacctctac gaccagctgg tcgaaaaggt aaccgaggtg cgcgaaaagg
     3481 gctgcacagc cacctgctag ggactaaccc taggtggcaa ggggcagggt ttgccgcccg
     3541 gcgcttttgc cgaacggcag aaaagctgat aaatgggcgc gtttcccgat gctgaaacgt
     3601 ggccgttccg gccgatttat ccttgaaaac aggcaaccgc tccggtcatg gcccagacga
     3661 tccaaatcct caacggccct aatctcaacc tgcttggcac gcgggagccc gagatttatg
     3721 gcagcgagac gttggacgat ttgcacgcgc gctgcgagcg caaggcgaag gcccttggct
     3781 atgaggtcgt gttccggcaa agcaaccacg agggcgagct ggtctcgtgg gtccaggagg
     3841 cgcgcgagcg tgccgcaggg ctgattgtca acgccggcgc cttcacccat acctcggtcg
     3901 ccctgctcga tgccttactg tcctgctcgg taccgattgt cgaagttcat ttatccaata
     3961 tcttccgccg cgagccattc cgtcaccggt cctatgtatc aaaggccgcc aagggcgtga
     4021 tctgcggatt cggaggcttg ggctacgagc ttgccatcga ggcgctcgcc acctcgctcg
     4081 cggaagccaa ggccggttga tggccaagaa aggacgccga tgaataaaga cgggggtaac
     4141 ctcgataaag acctcatccg agagctggcg gagctccttg aggagaccgg tctgaccgag
     4201 atcgagatcg agctcaagag cggccgcttc agggtggccc ggggtgtcaa tctcaccacg
     4261 acgctgatga cgaccggcaa cggcgcacca ccacctgcgc cgccgcaaga gcatcgcgag
     4321 ccgaagcgcg cctccgagac cgaagacgtc gccgtgcatc cgggctctgt gacctcgccc
     4381 atggtcggca ccgcctaccg gtcggccgaa cccggcgcgc cacccttcgt cgaggtcggc
     4441 agccgcgtgt ctgcgggtca aacactgctc atcatcgaag ccatgaagac gatgaaccac
     4501 attccctcgc ccaaggctgg cacggtcctc gccatccttg tcgaggacgg tcagccggtg
     4561 gaatacggcg agccgctcgt ggtgattgag cagggttagc cacgctctat gttcgacaag
     4621 atcctgatcg caaaccgggg agagatcgca cttcgcatcg aacgtgcttg caaggagctg
     4681 ggcatctcca cggtggcggt tcactccacg gcggatgcac acgccatgca tgtccgcctc
     4741 gcagacgaga gcgtgtgcat cggcgcaccg cccgccaagg acagctatct caacattccg
     4801 gccatcgtcg ccgcctgcga gatcaccggc gccgatgccg tgcatccggg ctacggcttc
     4861 ttggccgaga atgcccgctt cgccgagatc ctcgacgaac acaacatcgc gttcatcggg
     4921 cccacggccg agcatgtccg gctcatgggc gacaagatca aagccaagga aacggcgaag
     4981 tccctcggca ttcccgtcgt gccaggttcg gacgggccgg tcacgaacta cgacgaggcg
     5041 aaaaagatcg cggccaagat cggctttcca gttctggtca aggcgtccgc gggcggcggc
     5101 gggcgcggca tgaagctcgc gcatttcgag aacgagcttg agcctgcatt ggcgacggcg
     5161 cgcaacgaag cgcgcgcggc cttcggcgac gatacggtct atctggagaa gtatctcggc
     5221 cagccacggc atatcgaggt gcaggtctta ggcgatggcg aaggcaacgc cattcatctc
     5281 ggcgaacgcg attgttcgct gcaacggcgc aatcagaagc tttgggagga ggcaccctcg
     5341 cctgctctga acgacaacga acgcctacgc atcggcgaga tcgtggcgga tgcgatgcgc
     5401 aaactcaaat atcgcggcgt gggcaccgtg gagttcctct acgaaaacgg cgagttctac
     5461 ttcatcgaga tgaacacgcg gctccaggtg gagcataccg tgaccgagat ggtcaccggg
     5521 ctcgacctgg tgatcgagca gatcagaatc gcggccggtg cgccgctcac gctggcccaa
     5581 gaagacgtgt cgttcatcgg ccacgccatc gaatgccgcg tcaatgcgga gaacgcgctc
     5641 accttccggc cctcgccggg gacgatcacg catttccacc cgccaggcgg acttggcgtc
     5701 cgggtcgatt ccggcgctta cgcgggctac accattcccc cgtattatga cagtctgatt
     5761 gggaagctca tcgtgcacgc ccgcacgcgc aacgaatgct tgatgcggtt gaagcgggcg
     5821 ctcggagaat tcgtcgtcga tggcatcgag accaccattc cgctgttctc atcgctcatc
     5881 tcagagcccg acattgtcga cgggagctac gacattcatt ggctcgagga ccatctggcg
     5941 ccgaataccg aacgcccggg gtagcgcgtc atgaccacca tcgacgacgt gatgatcgag
     6001 atcacgccgc aggtgctgct caaggcgtat gcgtgcggca tctttccgat ggcagagagc
     6061 gcggatgaca acgcgctgta ttggatcgag ccggagcgtc ggggcatcct ccccttagac
     6121 gacgtacaca tccctaagag tctggcgcgc accattcgcc ggggcgggtt cgaggttaaa
     6181 gtcgaccacg acttcgaggg cgtcatcgac ggctgtgcgg cgccccgcgc gggccggcgc
     6241 agcacgtgga tcaacggccg catccgcagt ctctaccgcg aactgtttaa gctcggccat
     6301 tgccataccg tggaggtctg gcaggacggg aagatgatcg gcggtcttta cggcgtccac
     6361 ttaggccgcg cgttcttcgg cgagagcatg ttctccacgg cgcgcgacgc gagcaaaatc
     6421 gcgctggtct atctcgtcgc caggctaaag tacggaggct ttaccctgct cgacacgcag
     6481 ttcgtcaccg gacacctcgc ccgcttcggc gcgatcgagg tgagccgtca ggagtttcag
     6541 gggctcctgg aaggcgcgtt ggggagccag ggtagaccgc ccggaccggg cggtggcgcg
     6601 ggccgcggcc gcggtgccgc agcggcagga ggcggtgcag gcgcggcagc aggaggcggt
     6661 gctggcgcgg cgggcgcagg cgcaggctca gccatggcct caccttctgc gccaggttcc
     6721 gaaggcgcgt tctccgcgct ctccgaaggc gccccgccaa tcggcatctt gcagctggtg
     6781 agccaaacat cgaagaccgg atgctccacg gcgtgaagtc cagggctctc ggcgaacatc
     6841 cacccggtga agatgcgatg ctgctcgccc gcgagcgtaa cctcgt
//
LOCUS       DBNHVU010000030         5763 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_829602, whole genome shotgun sequence.
ACCESSION   DBNHVU010000030 DBNHVU010000000
VERSION     DBNHVU010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5763)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_829602"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..436)
                     /locus_tag="AC16Y7_01135"
     CDS             complement(29..436)
                     /locus_tag="AC16Y7_01135"
                     /inference="COORDINATES: protein motif:HMM:NF020157.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HIR5708012.1"
                     /translation="MAINYIAVVIATLAGFGLGAVWYMVLGRAWMRALDKTEAEIKEG
                     QGSVKALPFIIALVALFVMAWALAGLMGHLAQVNVRGGVIAGLIVWIGFVITTMGVNH
                     AFSGASPKLTLIDGGHWLAVLVVMGAVIGAFGL"
     gene            complement(499..930)
                     /locus_tag="AC16Y7_01140"
     CDS             complement(499..930)
                     /locus_tag="AC16Y7_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HIR5708013.1"
                     /translation="MAYWLMKSEPGTWSWEQQKNEGKKGAEWDGVRNYQARNNMRAMK
                     KGDFAFFYHSIGEKACVGIVKVVVEAHPDSTDTTGKWECVDVAAVADLPSPVTLADIK
                     DTPQLENMVLVKNSRLSVQPVTAAEWKKVCALGGLKDPPTA"
     gene            complement(939..1232)
                     /locus_tag="AC16Y7_01145"
     CDS             complement(939..1232)
                     /locus_tag="AC16Y7_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006280397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR5708014.1"
                     /translation="MLFAFICTDKPGGLPIRKENRPDHLAYLKSLGDALKFAGPFTAE
                     DGETMNGSLIVIEAASLQAARDIADDDPFAKAGLFASVEIRPWLWSLGKPGGA"
     gene            complement(1308..2303)
                     /locus_tag="AC16Y7_01150"
     CDS             complement(1308..2303)
                     /locus_tag="AC16Y7_01150"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR5708015.1"
                     /translation="MAHQSVGIIGAGAWGTALAIVARRAGRDVLVWAHEPETISAIND
                     QHCNEVFLPGVPLDPAIEATDRLSEAATCDMLLMVTPAQHFRPIAGELKPYVRESQPL
                     VICCKGIEQTTGKLMSQIAAELLPETATSVLSGPSFAGDVANGRPAAVTMSTEEEELG
                     RALSHALSHPPFRCYWSNDLIGAEIGGAAKNVYAIAAGIVVGKALGASAHAGLVTRGF
                     AEMARFAVALGGKLETLIGLSGLGDLVLTCGSPHSRNMSLGVALGEGKSAEEVLASRL
                     TVTEGVATAAAMAEIAGARGIELPIAEAVHAVLSGRTTVDAAIGGLLARPLRSEI"
     gene            complement(2361..3422)
                     /gene="tsaD"
                     /locus_tag="AC16Y7_01155"
     CDS             complement(2361..3422)
                     /gene="tsaD"
                     /locus_tag="AC16Y7_01155"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR5708016.1"
                     /translation="MLGIETSCDETAASVVRQGADGAGEILSNVVRSQLDLHAAYGGV
                     VPEIAARAHVDLLDTAVTQAMADFGLDFAALDGIAAAAGPGLIGGLIVGLTTGKAMAL
                     GLEKPFMAINHLEAHALSAGLTDGLDFPYLLLLVSGGHTQLLIVKDVGAYERLGTTLD
                     DALGEAFDKAAKLLGLPYPGGPEVERTAARGRATIKLPRPMLGRSEPHFSLAGLKTAL
                     RQEAINRAPLDDQTVADLCASFQEAVADVIQDRTARAMDLYATRLGEKAQRALVVAGG
                     VAANQRLRGALQDVARARGFSLVVPPPALCTDNGAMIAWAGTMRLARGLTDDLTAPAR
                     ARWPLDPDAPPALGAGVKA"
     gene            3553..4482
                     /gene="hemC"
                     /locus_tag="AC16Y7_01160"
     CDS             3553..4482
                     /gene="hemC"
                     /locus_tag="AC16Y7_01160"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HIR5708017.1"
                     /translation="MPATILIGTRGSPLALWQAAHVRDRLHQTHGLDRGAVDLRVITT
                     SGDRIEDKALRDFGGKGLFTKEIDEALFAGEVGLAVHSMKDLPTVLPQGISLAAVLPR
                     ADVRDAFLSPSAGSLADLPPGAVVGTSSLRRGAQVRRSRPDLRVIDFRGNVETRLKKL
                     DEGVADATLLAKAGLDRLGLTDKAASILETEEMLPAVAQGAIAILARDGDEETRRLLA
                     PLNDPATATAVACERAFLARLDGSCRTPIAGLAEIGDDKTLRFRGLILSPGGAEWHDV
                     EVTGAAEQAEQIGRDAAEDLLDRAGPNFLAQLP"
     gene            4491..5216
                     /locus_tag="AC16Y7_01165"
     CDS             4491..5216
                     /locus_tag="AC16Y7_01165"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HIR5708018.1"
                     /translation="MRLLVTRPEPDAGREADALIALGHDPVRAPLLEIEFLSDVPLPL
                     DDAQGVVITSRNTIRALANHPDVDQVLELPVFAVGEATAWAARQLGFREVTIGPGTGA
                     GLPPLIRREVHRGKGPLVHIGAEKVAFDLKGALEEDGFELRRIVLYRSHPVDTLTQEV
                     VSELKSGAISGVLLMSPRTARTFTRLAQAEGLVPEAKGLVCYCLSKAIAEAVAPLGLP
                     VRVPTYAREEDLLALLNSGASSS"
     gene            5252..>5763
                     /locus_tag="AC16Y7_01170"
     CDS             5252..>5763
                     /locus_tag="AC16Y7_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708019.1"
                     /translation="MAGSFDDKSSTSDQAGKRPAPTIEGTAKEVSVETEDKATDDASD
                     ETRTEKTESGETRALPSESDEDASETDEPESPAATRADQSDEGRRSFGASLLAALTSF
                     TTHAAAGLLGGALVLVGMGLGYVPTPTSTDPDEAAVLKDRIATLEAAPQTPDNSAALK
                     TLETRLAALE"
BASE COUNT          997 a         1929 c         1849 g          988 t
ORIGIN      
        1 gagaagacac cgcccattat cctttcgtct agagtccgaa agcgccaatc acggcgccca
       61 tgacgacgag aacggcgagc cagtggccgc cgtcgattag ggtcagcttg gggctggcgc
      121 cgctgaaggc gtgatttacg cccatagtgg tgatgacaaa accgatccaa acgatgagcc
      181 cggctatcac gccacctctg acgttgactt gcgccagatg gcccatgagc ccagccagcg
      241 cccaggccat gacgaacaag gctaccaggg cgatgatgaa gggcagcgcc ttgaccgagc
      301 cctgtccttc cttgatttcg gcttccgtct tgtcaagtgc gcgcatccag gcgcgcccga
      361 gcaccatgta ccacaccgcg ccaaggccga acccggccaa ggtcgcgatg acgacggcga
      421 tatagttgat ggccatgtca ttctccttcg cggaccacga tccggcgcaa ctctagcgca
      481 agccatgtgg taaaggcact acgccgtcgg cgggtccttc aggccgccaa gggcgcagac
      541 cttcttccac tccgccgcgg tgacaggctg caccgacagg cgagagttct tgaccagcac
      601 catgttctcc agctgggggg tgtccttgat atccgccagc gtcacggggc tcggcaaatc
      661 ggcgacggcg gccacgtcca cgcattccca cttgcccgtg gtgtcggtgg agtccgggtg
      721 cgcctcgacc acgaccttga cgatgccgac gcacgccttc tccccgatcg aatggtagaa
      781 aaaggcgaag tcgcccttct tcatcgcgcg catgttgttg cgtgcctggt agttccgcac
      841 gccgtcccac tcggcgccct tcttgccttc gttcttttgc tgctcccagg accaggttcc
      901 gggctcggac ttcatcagcc aatacgccat cacagttcct aagcgccgcc aggtttgccg
      961 agggaccaaa gccacggcct gatctcgacc gaggcgaaca ggccggcttt ggcgaacggg
     1021 tcgtcgtccg cgatgtcgcg cgcggcctgc agtgatgccg cctcgatcac gatcaggctg
     1081 ccgttcatcg tttcgccgtc ctccgcggtg aagggaccgg caaacttgag cgcatcgccg
     1141 aggcttttga gataagcgag atggtctggg cggttctctt tgcggatcgg tagtccgccc
     1201 ggcttgtcgg tacatatgaa cgcaaacagc atggatcccc cttggtttgc ggtgaggcta
     1261 ttgcgctgca ccggacctgt ccatggccga tcagatttca gatcgcgtca gatttcagat
     1321 cgtagcggcc gcgcgagaag tcccccgatc gcggcgtcca ccgtcgtccg gccggagagg
     1381 accgcgtgca cggcttcggc gataggaagc tcgatgccgc gcgccccggc gatctccgcc
     1441 atggcagccg ctgtcgccac gccttcggtg accgttagcc ggcttgcaag cacctcctcg
     1501 gcgcttttgc cctcgccgag ggcgacaccg agagacatgt tgcgcgagtg cggactgccg
     1561 caggtgagca ccaaatcgcc caggcccgaa aggccgatca gcgtctccag cttcccgcca
     1621 agcgcgaccg cgaaccgcgc catctccgca aagccgcggg tgacaagtcc cgcatgggca
     1681 ctcgcgccca gcgccttgcc cacgacgatg cccgccgcga tggcgtagac gttcttcgcc
     1741 gcgccgccga tctcagcgcc gatcaggtcg ttgctccaat agcagcggaa cggcggatgg
     1801 ctcagcgcat gcgacagcgc gcggcccagc tcctcctcct cggtcgacat ggtcaccgcg
     1861 gccggccggc cgttcgccac gtcgccggcg aagcttggcc ccgacagcac ggaggtcgcc
     1921 gtttcgggca acagctcggc cgcgatttgc gacatcaact ttccggtcgt ctgctcgatg
     1981 cccttgcagc agatcaccag gggctggctc tcccgcacat atggtttgag ttcgcctgcg
     2041 atggggcgga aatgctgcgc gggcgtcacc atcagcagca tgtcgcaggt ggcggcctcg
     2101 ctgagccggt cggtggcctc gattgccggg tcgagcggca cgccaggcag gaacacctcg
     2161 ttgcagtgct gatcgttgat tgcggaaatg gtctcgggct catgcgccca gacgagcacg
     2221 tcacgtccgg cgcgccgcgc cacaatggcg agcgcggttc cccacgcccc cgcgccgata
     2281 atgccgaccg attggtgcgc catgtgcccc ctcgactcac ttcatagcgc aatttgtgcg
     2341 tgtcaggcga acggaagcgc ctaagccttc actccggcgc ccagcgcggg cggtgcatcc
     2401 ggatcgagcg gccagcgcgc ccgggcgggc gctgtgagat cgtcggtcaa accccgggcc
     2461 agccgcatgg ttccggccca ggcgatcatc gcgccattgt cggtgcacag cgccggcggc
     2521 ggcacgacca gactgaaccc acgagcgcgg gccacgtctt gaagcgcccc cctgaggcgt
     2581 tgattggcgg ccacgccgcc agccaccacc agcgcccgct gtgccttctc accaagccgc
     2641 gtcgcgtaga gatccatggc ccgcgccgtt cggtcctgga tcacatccgc caccgcctcc
     2701 tggaagctgg cgcagagatc cgcgaccgtc tggtcatcca agggcgcgcg gttgatggct
     2761 tcctggcgga gcgcggtttt gaggccggcc agagagaaat gtggctcgct tcgccccagc
     2821 atgggccggg gcagtttgat ggtcgcgcgc ccccgcgcgg cggtccgctc gacctccggc
     2881 ccccccggat agggcaggcc cagcagcttc gccgccttgt cgaaggcctc gcccaaagca
     2941 tcgtccagcg tggtcccgag ccgttcatag gcgccaacgt ccttgacgat caggagttgg
     3001 gtgtggccgc cggagaccaa aagcagcagg tagggaaagt cgagcccgtc cgtgagcccg
     3061 gcgctcagcg cgtgggcctc caagtggttg atggccatga agggcttctc aaggccgagc
     3121 gccatcgcct tgcccgtggt gaggccgacg atcagcccgc cgatcaggcc cggcccagcg
     3181 gcggccgcga tcccgtccag cgcagcgaaa tccagcccga agtccgccat ggcttgggtg
     3241 accgccgtat ccagcaggtc cacatgggcc cgcgccgcga tctcgggcac gaccccgcca
     3301 taggcggcgt gcaggtcgag ctgcgagcgc accacgttgg acaagatctc gcccgcgccg
     3361 tccgcgcctt ggcgcaccac cgacgcagcc gtctcgtcgc aactcgtctc gatcccgagc
     3421 acggtcaggg cggaggcgtt ctttgtcatg cttcctttta tacggtccgc gcggagattg
     3481 aagccggcgc cgaaacaagc cattaatgcg gggacctggc ccctttatcc agacagctca
     3541 aggagttccg ccttgccggc aacgattctc atcggcaccc gtggcagccc gcttgccctg
     3601 tggcaggcgg cccatgtgcg cgaccggctg catcagaccc acgggctgga ccgtggggcg
     3661 gtggacctca gggttatcac cacgagcgga gatcggattg aggacaaggc gctgcgcgac
     3721 ttcggcggca agggcctttt caccaaagag atcgacgagg cgctttttgc aggcgaggtc
     3781 ggccttgccg tgcactccat gaaggatctg ccgacggtgc taccgcaagg gatttccttg
     3841 gccgccgtgc tcccccgggc cgacgtgcgc gatgcttttc tcagcccaag cgcagggtct
     3901 ctcgcggacc tgccacccgg cgccgtggtc ggcacctcat ctttacgccg tggtgcgcaa
     3961 gtgcgccgct cgcgtccgga cctgcgcgtc atcgacttcc gcggcaatgt cgaaacgcgc
     4021 ctcaagaaac tcgacgaagg cgtcgccgac gcaacactgc tggccaaggc gggcctcgac
     4081 cgtctcggcc tcacggacaa ggccgcatcg atcctcgaaa ccgaggagat gctccccgct
     4141 gtcgcgcaag gcgccatcgc catcctcgcc cgcgacgggg atgaggagac gagacgcctg
     4201 cttgcaccgc tgaacgatcc cgcaacggca acggcggttg cttgcgagcg cgcctttttg
     4261 gctaggctcg acggttcgtg ccgcacgccg atcgccggcc ttgccgagat cggagacgac
     4321 aagactttgc gttttcgtgg gttgatcctc tcgccagggg gcgcagaatg gcacgatgtc
     4381 gaggtgaccg gcgcggcgga gcaagctgaa cagattggcc gcgatgccgc tgaagacttg
     4441 ctggaccgcg caggccccaa cttcctcgcc cagctaccgt gatggatgcg atgcgtttgc
     4501 ttgtaacgag gccggagccc gatgcgggtc gtgaggccga cgcgctgatt gcactgggcc
     4561 atgatccggt gcgcgcgccg ctgctcgaaa ttgaatttct gtcagacgtg ccgctgccgc
     4621 tcgatgacgc gcaaggcgtc gtcatcacca gccggaacac gatccgcgcg cttgccaatc
     4681 acccggacgt ggatcaggtg ctggaactgc ccgtattcgc ggtcggtgag gcaacagcat
     4741 gggcggcgcg gcaactcggc ttccgcgaag tgacgatcgg tccaggaaca ggtgccggtc
     4801 tgccgccgct catccgtcgt gaggtgcacc ggggcaaagg tccgcttgtg catatcggtg
     4861 ccgagaaggt cgcattcgat ctaaagggtg cgctggagga ggatggcttc gaactgcggc
     4921 gcatcgtctt gtatcgctct catccggtcg acacgctaac gcaagaggtc gtctctgaac
     4981 tgaagagcgg cgcaatctcg ggtgttctgc ttatgtcgcc gcgaacggcc agaaccttca
     5041 cgaggctcgc gcaggccgag ggcttggtcc cggaagcgaa gggacttgtt tgctattgcc
     5101 tgtcgaaggc gatagcagag gccgtcgcgc ccttagggct accggttcgc gtgccgacat
     5161 atgcgcggga ggaagatctc cttgcgctcc tgaactccgg cgcgtcatcc tcgtgagtgg
     5221 cgcgaatctc tgatgcgaag gtgatgagct gatggccggc tctttcgacg acaagagcag
     5281 cacttccgat caagcgggca agcgtccggc gccaaccatc gagggcacgg cgaaggaagt
     5341 ctccgtggaa actgaggaca aggctacgga cgacgcgtcc gacgagacgc gtacggagaa
     5401 gaccgagagc ggcgaaaccc gcgcgcttcc ctcggagtcc gacgaggatg cgtccgagac
     5461 cgatgagccc gaaagcccag cggcaacgcg cgccgaccaa tcggatgagg gccggcgatc
     5521 attcggcgcg agcctcttgg ctgcgctgac cagtttcacg acccatgcgg cagcgggcct
     5581 tctcggcggc gcgctcgttc tcgttggcat gggtttgggt tatgtgccga cgccgacgtc
     5641 gaccgatcca gacgaagctg cagtgctcaa ggaccgcata gccacgctcg aggccgcgcc
     5701 gcaaacgccg gacaacagtg cggcgctcaa gaccctcgaa acccgtttgg cggccctgga
     5761 aag
//
LOCUS       DBNHVU010000031        10477 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1572487, whole genome shotgun sequence.
ACCESSION   DBNHVU010000031 DBNHVU010000000
VERSION     DBNHVU010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 10477)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10477)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10477
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1572487"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..337
                     /locus_tag="AC16Y7_01175"
     CDS             89..337
                     /locus_tag="AC16Y7_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708020.1"
                     /translation="MSTDKKTKPQFSRRPAKRVLTGETREEEEARLAAEIAAHPVTKC
                     EPGEYTSTSSRPGWNNKPIIPQADVATAERIAKKMRKS"
     gene            375..653
                     /locus_tag="AC16Y7_01180"
     CDS             375..653
                     /locus_tag="AC16Y7_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708021.1"
                     /translation="MMRFTIPVERGNQAFADHTIGEAIEALVKATNAEAAYFATINGK
                     RAGFIFFEETDQAQMAKLNEPMFAALNASIDTTPALTLEDLKRGLAQD"
     gene            663..995
                     /locus_tag="AC16Y7_01185"
     CDS             663..995
                     /locus_tag="AC16Y7_01185"
                     /inference="COORDINATES: protein motif:HMM:NF020037.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR5708022.1"
                     /translation="MQSSQLEVFNAMPFFFWAKDEEGRYVWGNRSINTFAKEDVAGKT
                     DYELPWSANADRLRTDDKEVLRTREPIFRREYVGKSGKGRTTLSVCKFLGELDGTRCV
                     MGVSFVIE"
     gene            complement(1004..1861)
                     /locus_tag="AC16Y7_01190"
     CDS             complement(1004..1861)
                     /locus_tag="AC16Y7_01190"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR5708023.1"
                     /translation="MARKRLNRIAFQGEPGANSHLAARDAYPNMEAMACATFEDALAA
                     VKTGKARFAMIPIENSVAGRVADIHHLLPHSGLYIVAEHFEPIRHQLLALPRAKLTGL
                     KTVHSHIMALGQCRKAIAALKLKPVTEADTAGAARHVREAGDPTQGAIASKLAAEIYG
                     LRIAKKNIEDAAHNTTRFVVLAAKPVRPKASDGPVLTTFVFRVRNVPAALYKAMGGFA
                     TNGVNMTKLESYQLEGSFNATMFYADIEGHPSERPVKLAMEELGFFTSQVTVLGTYPA
                     SPFRAKLKA"
     gene            complement(1900..2637)
                     /locus_tag="AC16Y7_01195"
     CDS             complement(1900..2637)
                     /locus_tag="AC16Y7_01195"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HIR5708024.1"
                     /translation="MAKSLIVIPARLAATRLPDKPMAEIAGEAMIVHVWRRAIEANCG
                     PVLVATDADSVFDAVAKAGGEAVMTRPDHASGSDRIHEALQQVDPDRRVDTIVNLQGD
                     LPTLDPGLVHTCVAALEEGNADIGTIAAEIVRDEERTNPNVVKVVGSPLPRGGILNAL
                     YFTRATAPHGNGPLYHHIGIYAYRRAALERFVALPPSPLETREKLEQLRALEAGLRIH
                     VGLVDTVPLGVDTPADLARAREILTSA"
     gene            2792..4351
                     /locus_tag="AC16Y7_01200"
     CDS             2792..4351
                     /locus_tag="AC16Y7_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5708025.1"
                     /translation="MKYYYLNRIAMAVLVALLLFFGTRTLIDIVYQEHPPEKPGYEVA
                     GTKDSDGEKKKEPADEGAAFVAALNKADPAKGEQDVGLCKVCHSFEKGGPTMIGPNLY
                     GIVGMKIASHEGVNYSPALKGHGGDWTFENMDKWLTNPQAFAAGTTMAFPGIPDLAKR
                     ANIVAYLNKNSDDPLPIPEPKAAPEAADEGAAEDAAPAKPEVFALLATADAAKGEQAA
                     ALCKVCHTFEKGGAAMVGPNLHNVVGADVAHVEGFNYSAALKGHGGKWTYEALDAWLK
                     NPQEFAAGTTMAFPGVPDAKKRADIIAFLMSHTDNPPPVPSGEAEPAAPAEEAKPAED
                     EAKPAEEPAAEEEPPFAAEPEEPSVTDEPAADEVMEKGREEIIINAPTVSDDAPSEPH
                     KGEAPSPNQPQPVVPEDSGDDAAASDGDAAMEEASEVMSAPPTVSPDAPSEPHMGEAP
                     SPNQPQPVVPDDSASSHSMAPASDDGMSSQPQPVYPDGEPGSASMAPAGGDGPSNQPQ
                     PVYPDGAPDGL"
     gene            4531..6402
                     /locus_tag="AC16Y7_01205"
     CDS             4531..6402
                     /locus_tag="AC16Y7_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5708026.1"
                     /translation="MKLSSAPMIVRRLTFAALLTALVLSSATAEESAKHHALSLIGEP
                     KYKAGFTHFDFVNPDAPKGGTVRLPSIGGFDSLNPVLYRGEKAAGLQLVYESLMHDSI
                     DEPSTSYGLIAEWASYPEDYSSVTFKLRDDARWHDGEPITPDDVIYSLEINKTANPRM
                     GLYYKNVSRAEKTGDNEVTFYFDTKGNRELPMIMGQLTILPKHWWTGKDGSGAARDPM
                     KTTMELPLGSGPYRIGTVSPGRSISYERVDDYWGKDLPVNKGQWNYDEVRFESYRDQT
                     VAFESFKAGNLDYWTESSAKNWATAYDFPALRDGEVKREEVKIKRTMPMQAFVMNLRR
                     PQFQDRRVRQALNLAFDFEWANKNLFYDQYERVGSYFQNSELAAPSELPAGRELEILE
                     EVKGEVPEEVFTKVHKNPVNEDRNSMRKNLRQAVMLLKDAGWQVKDGVLVNTETGEKM
                     TIEFLLVSPLFQRIVQPYMRNLERLGIKSTLRLVDSAQYTRRLNGYDYDIVVGNFAQS
                     DSPGNEQRDFWGSEAADRDGSRNLIGIKDPAIDKLIDRVIFAKDRAELVAATRALDRV
                     LLWHDFVVPQWFSPNVRIAYWNRYDQPKKLPGLTPGFLQVWWYDKTKAEELAEPSKS"
     gene            6402..8369
                     /locus_tag="AC16Y7_01210"
     CDS             6402..8369
                     /locus_tag="AC16Y7_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5708027.1"
                     /translation="MAMARAKSSLIVAALALALGTSAVTAAQRNHGLSAFGDLALPQD
                     FKHLAYADPEAPKGGMLVLIGWGGVTTFNNLNNYILKGDAAQGLDLLFDSLMTRAMDE
                     PDAVYGLVAESAEVADDGMSVTFYLRPEARFADGSPLTADDVAFSFETLKTKGHPLFR
                     QTLRDVENVEAVDPHTVRYTLKGNQVRDLPLTVAALPVLSKAYYTKRKFDETTMEPPV
                     GSGPYVIDDFAQGRTITYKRNPDYWAKDLPVNAGRWNFDRIRFEYFRDRTAGMEAFKA
                     GTYDFREEFTSKVWATEYDFPAVRDGRVKKETLLDETPSGTQGFFINTRRDKFKDPRV
                     REALALAFDFEWTNRNLFYGLYDRTESYFENSPMKAEGEPSAEEHTLFESLEVEVPEA
                     ALRSAAIPPKSDGSGQDRRLLLKAGGLLDDAGWRIENKKRVNDKGEQLTVEFLTFEPT
                     FERIVAPYVKNLKLLGIDSRIRRVDPAQYQQRLKNFDFDITTQRYVMRNTPGVELRSY
                     FGSQAADLDGSLNLAGVKDPAVDALIEKVVTAKTREDMTIAARALDRTLRAGHYWVPH
                     WYKGSHTVAYWDKFGRPETKPKFDRGLLDTWWFDEAKASEVATNGHSRPSQDGNGTEG
                     ESSRLWLILGGLALAVLAVTLFFRGRRKARG"
     gene            8522..9220
                     /locus_tag="AC16Y7_01215"
     CDS             8522..9220
                     /locus_tag="AC16Y7_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR5708028.1"
                     /translation="MNSLGFAAIAAGIILYALFAKRLETTVITPPLVFAVFGLVVGES
                     VLGWADLSFENHFIHGLAEITLILVLFSDAARIDLSLLRKDHNLPLRMLVIGMPLIII
                     FGTVAALALPLGFNLWEAALLAAILAPTDAALGQSVVLSRFVPARIRQTLNVESGLND
                     GIALPFVLIFAFLAGAPCRRKVVTIGSSSAPSRSFSAPWRASLSAWPARGFWTWPPKK
                     NGRRRATKVPQSSV"
     gene            9115..9789
                     /locus_tag="AC16Y7_01220"
     CDS             9115..9789
                     /locus_tag="AC16Y7_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR5708029.1"
                     /translation="MAGVVVGMAGAWLLDLAAKKEWTTASYEGAAILGLAFLAYSAAE
                     LIGGNGFIAAFVSGLVFGNFVRGRCGFLFEFAEAEGNFLVLLTFLIFGAAILPSAFGS
                     FDLVMLVYAVLSLTVIRMIPIAIALAGSGVSSPTVLFLGWFGPRGLASILFALFVLEE
                     VAIPGGEDIAVTTILTVALSIVLHGLTAAPLSRIYADCIECAGPSEETEPVSEMPLRG
                     RAQPTA"
     gene            9955..>10477
                     /locus_tag="AC16Y7_01225"
     CDS             9955..>10477
                     /locus_tag="AC16Y7_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease family protein"
                     /protein_id="HIR5708030.1"
                     /translation="MAAYILRRLLLIVPTMLGIMLVTFVIVQFAPGGPVERLIAQLAG
                     TDPGATAKFSGGGGDFSGSGAQPGAVPGDVAAAPSKYRGAQGLDPEFIASLEKQFGFD
                     KPAHERFGKMIWDYLRFDFGESYFRDTSVLKLIAERMPVSISLGLWMTLISYSISIPL
                     GIRKAVKDGSRFDV"
BASE COUNT         2046 a         3263 c         3260 g         1908 t
ORIGIN      
        1 tgggtgcggg cggccgcttc tggtaaaggc accccagttt gaccacatca gtgcagtgga
       61 ttcctcgcat tccaatcgga gacaaacaat gagcacagac aaaaagacga agcctcagtt
      121 ctcgcgcagg cccgctaagc gtgtattgac aggcgaaacg cgcgaggaag aagaggcccg
      181 gcttgcagca gagatcgccg ctcatccggt gacgaagtgc gagcccggcg agtacacgtc
      241 cacctcgtcg cggccgggct ggaacaacaa gccgatcatt ccgcaagcgg acgtcgcgac
      301 cgcggagcga atcgccaaga aaatgcgcaa gtcgtgacgg cgaatttcca gccaagggag
      361 tttccatgcg gctgatgatg cgatttacga ttcctgtcga gcgcggcaac caggccttcg
      421 ccgaccacac gatcggcgag gcgatcgagg cgctggtcaa agccaccaac gccgaggcgg
      481 cctatttcgc gacgatcaac ggcaaacgcg ccggctttat cttttttgag gagaccgacc
      541 aagcgcagat ggcgaaattg aatgagccaa tgtttgccgc tctcaacgcc tccatcgaca
      601 cgacgcccgc gctgaccctc gaggacctga agcgcggcct ggcgcaggac taggacacga
      661 ccatgcagag cagccaactc gaagtcttca atgccatgcc cttcttcttc tgggccaagg
      721 atgaggaagg ccgctacgtt tggggcaacc gatccatcaa cacgtttgcc aaggaggacg
      781 tcgccggcaa gaccgattac gaactcccat ggtcagcaaa cgccgaccgg ctgagaaccg
      841 acgacaaaga agtcttgcgc actcgtgagc ccattttccg acgcgagtat gtcggcaagt
      901 ccgggaaggg ccggacaacc ctgagcgtat gcaagttctt gggagagcta gacggcacga
      961 gatgcgtcat gggtgtctcg ttcgtgatcg aatagcccgc agctcaggcc ttaagcttgg
     1021 cgcggaaggg gctcgccgga taggtgccga gcacggtcac ttggctggtg aagaaaccga
     1081 gctcctccat ggcgagcttg acgggacgct cgctcggatg accctcgatg tcggcataga
     1141 acatggtcgc gttgaacgag ccttcgagct ggtaggattc cagcttcgtc atattcacgc
     1201 cgttggtggc gaaaccgccc atcgccttgt agagcgcggc cggcacgttg cgcacccgga
     1261 acacgaatgt ggtgagcacc ggaccgtcgg acgccttggg cctgacgggc ttggccgcca
     1321 gcacgacaaa gcgcgtcgtg ttgtgggcgg cgtcctcgat gttcttctta gcgatgcgaa
     1381 gcccgtagat ttcagcagca agcttcgagg cgatagcacc ttgcgtcgga tcgcccgcct
     1441 cgcgcacatg acgcgccgcc cctgccgtgt cggcctcggt gactggcttc agcttgagcg
     1501 cggcaatggc cttccggcac tggcctagag ccatgatgtg actgtgcacg gtcttgagac
     1561 cggtcagctt ggcgcgcgga agcgccagca attgatggcg gatgggctcg aagtgctcgg
     1621 cgacgatgta gaggcccgag tgcggcagaa gatgatgaat atcggcaacc cgccccgcca
     1681 ccgaattctc gatggggatc atggcgaacc tcgccttgcc ggtcttcacg gcggccagcg
     1741 cgtcctcgaa ggtcgcgcaa gccatcgcct ccatgttcgg ataggcgtcg cgcgcggcca
     1801 aatgcgaatt ggcgccaggc tcgccctgga aggcgattct attcaagcgc tttctggcca
     1861 taggtcttgc ttggaaatgc tttgaaaaac gttgggactt caggcggatg ttagaatttc
     1921 acgcgcccgc gcaaggtcgg ccggagtatc gacaccgagc ggaaccgtgt caacgagccc
     1981 gacatgaatc cgtaagcccg cttccagcgc acggagctgc tccagcttct cccgggtctc
     2041 cagcggcgaa ggcggcagcg caacgaaacg ctcgagcgcc gcgcgccgat aggcgtatat
     2101 gccgatgtga tggtagagcg ggccgttgcc gtgcggcgcc gtggcgcggg tgaaatagag
     2161 cgcattcaag atgcccccgc gcggaagcgg cgagcccacg accttcacca cgttcggatt
     2221 ggtgcgctct tcgtcgcgca cgatctccgc cgcgatggtc ccgatatcgg cgttgccctc
     2281 ctccagcgcc gcgacgcaag tgtgcacgag gccgggatcg agcgttggca aatccccttg
     2341 caggttgacg atggtatcga cccgccggtc gggatcgacc tgctggaggg cctcatgaat
     2401 tcggtcggaa ccggaggcgt ggtcgggccg cgtcatcacc gcttcgccgc cggccttggc
     2461 caccgcatcg aacaccgaat cggcatcggt tgcgacgagg accgggccgc aattcgcctc
     2521 gatggcgcgc cgccacacat gcacaatcat ggcctccccg gcgatctcgg ccatgggctt
     2581 gtccggcaag cgggtcgcgg cgaggcgcgc agggatgaca atgagggatt ttgccatgat
     2641 tttccgctag ttggtttggg aagagttcca caccacaggt tgagtgtgcg gcaattctct
     2701 gagttgcaac agctggcgac cccttatagt ctcggcccgc gtcgcaagag ccacgaagaa
     2761 gtggactttg cctgccgggg ggacatcgac catgaagtat tactacctca atagaattgc
     2821 catggcggtg cttgttgcac tgctgctgtt tttcggcaca cgaactctca tcgatattgt
     2881 ttatcaggag catccgcctg agaagcccgg ctatgaggtg gctggcacca aggattcgga
     2941 cggcgaaaaa aagaaggagc cagctgacga aggcgcggcc ttcgtcgcgg ccctcaacaa
     3001 ggccgatccc gccaagggtg aacaggatgt cggcctctgc aaggtttgcc acagcttcga
     3061 gaagggcggc ccgacgatga tcggcccgaa cctctatggc atcgtcggca tgaagatcgc
     3121 gagccacgag ggcgtcaact attcgccggc cctcaagggc catggtggcg actggacctt
     3181 cgagaatatg gataagtggc tgacgaaccc gcaagccttc gcggccggca cgacgatggc
     3241 ctttcccggc atccctgacc ttgcgaagcg tgcgaacatc gtcgcctatc tgaacaagaa
     3301 tagcgacgac ccgctgccga tccccgagcc taaggcggcc cccgaggccg ctgacgaagg
     3361 cgcggcggag gacgcggcac cggccaagcc ggaggtcttc gcgctgctcg ccacggcgga
     3421 cgccgccaag ggcgagcaag ccgcggcact ctgcaaagtg tgccacacct tcgaaaaggg
     3481 cggggcggcc atggtcggcc cgaacctgca caacgtcgtc ggcgcggacg tcgctcacgt
     3541 cgagggcttc aactactccg cggccttgaa gggccatggc ggtaagtgga cctacgaagc
     3601 gctcgatgcg tggctgaaga atccgcaaga gtttgccgcc ggcaccacca tggctttccc
     3661 cggcgtcccg gacgccaaga agcgcgccga catcatcgcc ttcctgatga gccatacgga
     3721 caatccgcca ccagttccgt cgggcgaggc tgagccggct gcgcctgccg aggaggccaa
     3781 acctgccgaa gacgaggcga agcccgccga ggaacctgcc gcggaagagg agcccccgtt
     3841 cgcggcagag cccgaggagc cgtccgtcac ggacgagcct gcggccgacg aggtgatgga
     3901 aaaaggccgg gaagagatca tcatcaatgc gccgacggtc tcagacgatg ccccgtcgga
     3961 gccgcataag ggcgaggcgc cttcgccgaa ccagccgcaa ccggtcgttc cggaagatag
     4021 cggggacgat gcggcggcca gcgatggcga tgccgccatg gaagaggcca gcgaagtcat
     4081 gtcggcaccg ccgacggtct ctccagacgc accgtccgag ccgcatatgg gcgaggcacc
     4141 gtcgccgaac cagccgcagc cggtcgttcc cgacgatagc gccagcagcc actcgatggc
     4201 tccggccagt gatgacggga tgtcgtcgca gccgcagccc gtctacccgg atggcgaacc
     4261 gggcagcgcc tccatggcgc cggccggggg cgatggtccg tcgaaccagc cgcagccggt
     4321 ttatcccgac ggagcgccgg acgggctcta gaccaaagca aactggattt gagaaagggc
     4381 ctctattgaa ggggcccttt ttctttgggc gcttcgacat gaccgataat tcatttggtc
     4441 gcaccgcccg ccatgctagc ttttgccgag tttttcgcgc ctttggtcct tcttcgcgcc
     4501 cgcgcgttct tgggggccaa tccctcaaac atgaagcttt cttctgcgcc catgatcgta
     4561 cgacgcctaa cctttgccgc cctcctcacc gctcttgtgc tcagctcggc caccgccgaa
     4621 gagagcgcaa aacatcacgc gctgtccttg atcggcgagc cgaagtacaa ggccggcttc
     4681 acgcatttcg actttgtgaa tcccgacgcc cccaaaggcg gcacggtgcg cttgccctcg
     4741 atcgggggct tcgacagctt gaacccagtg ctttatcgcg gcgagaaagc agctggattg
     4801 cagctcgttt acgagagcct gatgcatgac agcatcgacg agccgtcgac ctcctacggg
     4861 ctcatcgccg aatgggcgag ctaccctgaa gactactctt cggtgacctt caagctgcgc
     4921 gatgacgcgc gctggcacga cggagagccg atcacgccgg acgacgtcat ctacagcctg
     4981 gagatcaaca agaccgccaa tccgcggatg ggtctctact acaaaaacgt ctcccgcgcg
     5041 gagaagaccg gcgacaacga ggtgacgttc tacttcgaca ccaaaggcaa ccgcgagctg
     5101 ccgatgatca tggggcagct caccatcctg ccgaagcact ggtggacggg caaagatgga
     5161 agcggcgcag cgcgcgatcc gatgaagacg acgatggagc tgccactcgg ctcaggccct
     5221 taccgcatcg ggaccgtcag tccgggacgc tccatttcgt acgaacgcgt ggacgactat
     5281 tggggcaagg acctgcccgt gaacaaaggg cagtggaact acgacgaggt gcgctttgag
     5341 tcctatcgcg atcagacggt cgcgtttgaa agcttcaagg ccggcaatct cgactactgg
     5401 acggagtcga gcgccaagaa ctgggcgacg gcctacgact ttcccgcgct tcgcgacgga
     5461 gaggtcaagc gcgaagaggt caagatcaag cgcacgatgc caatgcaggc cttcgtgatg
     5521 aatttgcgcc ggccgcaatt ccaggaccgg cgcgtgcggc aggcgctgaa cctcgccttc
     5581 gatttcgagt gggccaacaa gaacctcttt tacgaccagt acgagcgcgt cggcagttac
     5641 ttccaaaact ccgaactcgc ggcgccttcc gaactcccgg ccggccggga actggagatc
     5701 ctcgaagagg tgaaaggcga agtcccggag gaagtcttca ccaaggttca caagaacccg
     5761 gtgaacgagg accgcaacag catgcgcaaa aatttgcgcc aggcggtgat gctcctgaag
     5821 gatgccggct ggcaggtgaa ggacggcgtc cttgtcaaca ccgagacagg cgagaagatg
     5881 acgatcgaat ttctgctggt gtcgcccctg ttccaacgga tcgtgcagcc ctacatgcgc
     5941 aacctcgaac ggctcggcat taagagcacg cttcgcctcg tggactccgc gcagtacaca
     6001 cgccggctca acggctacga ctacgacatc gtcgtcggta actttgctca atcggactcg
     6061 cctggaaacg agcagcgcga tttctggggc tcggaggccg cggaccgcga cggcagcagg
     6121 aacctgatcg gcatcaagga cccggctatc gacaagctca tcgaccgcgt catcttcgct
     6181 aaggaccggg cggagctggt cgcggcaacg cgcgcactcg accgcgtgct gttgtggcac
     6241 gacttcgtgg tgccgcagtg gttctcgccc aatgtgcgta tcgcctattg gaaccgttac
     6301 gaccaaccta aaaaacttcc cgggctgacg ccgggcttcc tgcaggtgtg gtggtacgac
     6361 aagaccaagg ccgaggaact cgcggagcca tcgaagtcct gatggcgatg gcgcgcgcca
     6421 agtcctctct tatcgtcgcc gctctcgcgc tggcgctcgg gaccagcgcc gtcaccgcag
     6481 cccaacgcaa ccacggcctt tcggccttcg gcgatctcgc gctaccgcaa gacttcaagc
     6541 acctcgccta cgccgatcca gaggcgccga aaggcggcat gctcgttctg atcggttggg
     6601 gcggcgtgac cacgttcaac aatctgaaca actacattct caagggcgac gcggcgcagg
     6661 gcctggactt gttgttcgat tcgctcatga cccgtgcgat ggacgagccc gacgcggttt
     6721 acgggctcgt cgcagaaagt gccgaggtcg ccgatgacgg catgtcggtc acgttctact
     6781 tgcggcctga agcgcgcttc gccgacggca gtccgctgac cgccgacgac gtggcgttct
     6841 cgttcgaaac cttaaagacc aagggtcatc ctctgtttcg tcagacactg cgcgacgtgg
     6901 agaatgtgga ggccgttgat ccgcacaccg tgcgctatac cttaaagggc aatcaggtgc
     6961 gcgacctgcc gctgaccgtc gccgccctgc cggtgctctc caaggcctac tacacgaaac
     7021 ggaaattcga tgagacgacc atggagccgc ccgtcggctc cgggccttat gtgatcgacg
     7081 acttcgccca agggcgcacc atcacctaca aacgcaaccc cgactactgg gccaaggacc
     7141 tgccggtgaa tgcggggcgg tggaatttcg acaggatccg cttcgagtat ttccgtgacc
     7201 gcaccgccgg catggaggcc ttcaaggccg gcacatatga cttccgcgag gagttcacct
     7261 caaaagtgtg ggcgacggag tacgactttc cggccgttcg cgatgggcgg gtgaagaagg
     7321 agacgctgct ggacgaaacg ccgtcgggca cgcaaggctt cttcatcaac acacggcgtg
     7381 acaagttcaa ggacccgcgc gtgcgtgaag cgctggcact cgccttcgac ttcgagtgga
     7441 ccaaccgcaa tttgttctat ggcctctacg accgcacgga gagctatttc gagaactcgc
     7501 cgatgaaggc ggaaggagag ccgtcagcag aggagcacac gctgttcgaa agcttggagg
     7561 tggaggtgcc ggaggccgcg cttcgttctg ctgcgatacc gccaaagagc gatggctccg
     7621 gccaggaccg caggcttctg ctcaaggcgg gcggtttgct cgatgacgcg ggctggcgca
     7681 tcgaaaacaa gaagcgcgtg aacgacaagg gcgaacagct cacggtcgaa ttcctgacat
     7741 tcgagccgac cttcgagcgg attgtcgccc cctacgtcaa gaatctgaaa ctactcggta
     7801 tcgactcgcg catccgcagg gtcgatcccg cgcaatatca gcagcgcctc aagaatttcg
     7861 atttcgacat caccacgcag cgctatgtga tgcgcaacac gccgggcgtc gagctgcgaa
     7921 gctatttcgg ctcgcaggcg gcggacctcg acggctcgct caatcttgcc ggggtcaagg
     7981 acccggcggt cgacgcgctg atcgagaaag tcgtgaccgc gaagacgcgc gaggacatga
     8041 ccattgcggc ccgcgcgctc gaccggacat tgcgcgctgg gcactactgg gtgccccatt
     8101 ggtacaaagg ctcacacacg gtcgcctatt gggacaagtt cggccgcccc gagacgaaac
     8161 cgaaattcga ccgcggtctc ctcgacacct ggtggttcga tgaggccaag gcgtctgagg
     8221 tggccaccaa cgggcattcg cggccgtctc aagacggcaa tgggacggaa ggagaaagtt
     8281 cgcgcctctg gctgatcctg ggcgggctgg cgctcgccgt tctcgccgtt accctgttct
     8341 tccgcgggcg gcgcaaagca cgcggctaag ccaaacgatc gaagcgctcg atcttgcgaa
     8401 atacaggggc cggattcata cagatcggcg acctcggcat cggggaagct ggcttccagg
     8461 ccttccatcg gcgatgatcg gggtctgccg gatctctagg gctcggggac ctcgatcatc
     8521 catgaattca cttggctttg ccgcgattgc cgcgggcatc atcctctacg cgctgtttgc
     8581 aaagcggctc gaaacgaccg tcatcacgcc gccgctagtc ttcgcggtct tcggcctggt
     8641 cgttggcgag tctgttctcg gatgggccga tctgtcattc gagaaccatt tcatccatgg
     8701 gcttgccgag atcacgctga tccttgtgct gttctccgat gccgcgcgga tcgatctcag
     8761 cctcttgcgc aaggaccaca atctgccctt gcgcatgctg gtgatcggta tgccgctgat
     8821 catcatcttc ggcacggttg ccgctcttgc ccttccgctt ggcttcaatc tttgggaagc
     8881 ggcgctgtta gcggccattc tcgcgccgac cgacgcggcc ctcggacaat cggtggtcct
     8941 cagccggttt gtcccggcgc gcatccgcca gacgttgaac gtggagagcg gcctcaacga
     9001 cggcatagcg ctccccttcg tcttgatctt cgccttcctt gccggggccc catgcagacg
     9061 gaaagtggtc acaattggct catcttcggc gccaagcaga tcattttcgg ccccttggcg
     9121 ggcgtcgttg tcggcatggc cggcgcgtgg cttctggacc tggccgccaa aaaagaatgg
     9181 acgacggcga gctacgaagg tgccgcaatc ctcggtctag cctttctggc ctactcggct
     9241 gcggagttga ttggcgggaa cggttttatt gccgccttcg tcagtgggct ggtcttcggc
     9301 aatttcgtac gcgggcgttg cggctttctg ttcgagttcg ccgaagcgga gggaaacttc
     9361 ctcgtgctgc tgactttcct gatcttcggc gcggcgatcc tgccctcggc attcgggtcg
     9421 ttcgatttgg tcatgctggt ctatgcggtc ctcagcctta cggtgatcag gatgatcccg
     9481 atcgccatcg ctcttgccgg ctcaggggtg agcagcccga ccgtcctgtt cctgggttgg
     9541 ttcggaccgc gcgggcttgc ctcgatcctg tttgccctct tcgtcttaga agaggtggcg
     9601 atcccgggag gcgaggacat cgcggtgacg acgatcttga cagtcgccct cagtatcgtt
     9661 ctgcacgggc tcaccgcggc cccgctatcg cgcatctacg cggactgtat cgagtgcgcc
     9721 ggtccgtctg aggaaaccga gccggtgagc gagatgccgc tgcgcgggcg ggcccagccc
     9781 acagcctgaa gggcgcgaag gccttaaatc ttaccaaatt gcaatgactc cagacccttc
     9841 catcgggttt ccatgccgcc cagaatccag taagcatcag acttccgatg aggttcccgg
     9901 gccgccacgg cggggcggcg accgtcaggt ttagcgacag gaaacccagc gacattggcc
     9961 gcctatatcc ttcgcaggct tcttctgatc gttcccacga tgctcggcat catgctggtg
    10021 acgttcgtca tcgtgcagtt tgcgccgggc ggccccgtgg agcggctgat cgcccagctg
    10081 gccggcaccg atcccggcgc cacggctaaa ttcagcgggg gcggcggcga cttttccggt
    10141 agcggcgcgc agccgggcgc cgtgccgggc gacgtcgcgg cggcaccgtc caaatatcgc
    10201 ggcgcgcaag gcctggaccc ggagttcatc gccagcctgg agaagcagtt cggcttcgac
    10261 aagccggcgc atgagcgctt cggcaagatg atctgggact atttgcgctt cgatttcggc
    10321 gagagctatt tccgcgacac gagcgtcttg aagttgatcg ccgagcgcat gccggtctcg
    10381 atctcactcg ggctctggat gacgttgatc tcgtactcca tctccatccc gctcggcatc
    10441 cgcaaggccg tcaaggacgg ctcacgcttc gacgtat
//
LOCUS       DBNHVU010000032         4071 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1201760, whole genome shotgun sequence.
ACCESSION   DBNHVU010000032 DBNHVU010000000
VERSION     DBNHVU010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4071)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1201760"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="AC16Y7_01230"
     CDS             complement(<1..1228)
                     /locus_tag="AC16Y7_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5708031.1"
                     /translation="MSGAHDAKVIETSVPARLDRLRWGRFHTLIVLALGITWVLDGLE
                     VTLAGAVAGALKESPVLKLTNAEVGLASSFYLAGAVLGALLFGWLTDRLGRKKLFFIT
                     LAVYLIATAATAFSWNFVSFALFRFLTGAGIGGEYTAIASTIQEFIPARYRGHTDLVI
                     NGSFWIGAAGGAAGSIVLLDPAVLPPDYGWRAAFFIGSVLGLGILIMRRWIPESPRWL
                     MTHGRPDEAHEILEQIEDHHRARGHHVPDDDPALFKLNTRSHTPLAEVWHTLFDLHRQ
                     RTLVGLALMAAQAFFYNAIFFTYALVLTDFYGIAASKVGWYLLPFAAGNFLGPLLLGR
                     LFDTVGRRPMIAITYALSGLLLALTGALFAAGVLSATMQTVCWTVIFFFASAAASSAY
                     LTVSETFPLEIRALAIA"
     gene            complement(1225..1926)
                     /locus_tag="AC16Y7_01235"
     CDS             complement(1225..1926)
                     /locus_tag="AC16Y7_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013586.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIR5708032.1"
                     /translation="MSAGSMPKGFVYLYDVDPSIEQDMRYAGPNNFTRQPVPGYDAAE
                     CVLVREAAEALKAAQTDLKPMGLSLKVYDCYRPKQAVTAFVDWSKEPDNPNAKLEHYP
                     DLHKSALFPEYIATRSGHSRGSTLDVTIVPLGSSPADTDDEDGIACTEAQPSDNSVDM
                     GTGFDCFDPKANTDAPGLTPEQQRNRTLLVDVMSRHGFKNYPKEIWHYTFEPEPYPDT
                     YFDFPIVPRPASGKS"
     gene            complement(2307..2669)
                     /locus_tag="AC16Y7_01240"
     CDS             complement(2307..2669)
                     /locus_tag="AC16Y7_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HIR5708033.1"
                     /translation="MEINTLILGVATLLFAVALIALLVWAFKTFFGKSGGSTRRAPEK
                     RLTVVETAEVDSKRTLYLLRRDDVEHLVMIGGPVDVVVETGIRWRPQPQPQPLGPPHD
                     DVIIAKTETPGRPEYPKP"
     gene            2834..>4071
                     /locus_tag="AC16Y7_01245"
     CDS             2834..>4071
                     /locus_tag="AC16Y7_01245"
                     /inference="COORDINATES: protein motif:HMM:NF020037.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR5708034.1"
                     /translation="MTDINAPMRYAEPVADQTERDGSISLVVLLAVALAVAAVGLAMV
                     SREMAEPFVLAILAGLAVVGVFCLFAGAVGILYFGQRHARNDLTKAFVDNLPQGALIA
                     DATGRVLYANRAYRDLLGLDGEASVLAPDRAFGGNPHLAEPIFRLARAAQQGRRLKEE
                     FRLPPPGEVQDEGGITPRWFRISVQPMPPDPKAGRKGALVVWQVDEITQDRAREETSF
                     AKVQAAIDYLDNAPAGFFIADADGHIDYLNATLSQWLGLDLSEVSNRQIKLTQIMSED
                     SAALIAGASRGDTQGGTRHFDIDLLKANGTSLPVRLLLRLPRGSELAHVLILNRGPGE
                     VEEAGAGELRFARLFHSSPIAIATVDTTGTLSATNAAFVRMFGAETGDGETSLESFLD
                     PSNHADLRKALESAIAGQVLT"
BASE COUNT          777 a         1255 c         1333 g          706 t
ORIGIN      
        1 aggcgattgc gagcgcgcgg atttcgagcg ggaaggtctc gctcaccgtg agataggccg
       61 agctcgccgc ggccgaggcg aagaagaaga tcaccgtcca gcagaccgtc tgcatcgtcg
      121 ccgacagaac gcccgcggcg aatagcgcgc cggtgagcgc cagcaacaga ccggagagcg
      181 cgtaggtgat cgcgatcatg gggcggcgtc ccaccgtgtc gaacaagcgt ccgaggagaa
      241 gcggtccgag gaaattgccg gccgcgaagg gcagcaaata ccagccgacc ttgctggcgg
      301 cgatgccgta gaagtcggtc agcaccagcg cataggtgaa gaagatcgcg ttgtagaaga
      361 acgcttgcgc cgccatcagc gcaagcccca ccagcgtgcg ctggcgatgc aggtcaaaca
      421 gcgtgtgcca gacttccgct aagggcgtgt ggctgcgcgt gttgagcttg aacagtgccg
      481 ggtcgtcgtc gggtacgtgg tggccgcgcg cgcgatggtg atcttcgatc tgctccagga
      541 tctcgtgcgc ttcgtccggg cgcccatggg tcatgagcca gcgtggcgat tccggaatcc
      601 accggcgcat gataaggatg cccagcccca gcaccgagcc gatgaagaag gcggcgcgcc
      661 agccatagtc cggcggcagc acggccgggt cgagcagcac gatggaccca gcggcgcctc
      721 ccgccgcgcc aatccaaaag ctgccgttga tgacgagatc ggtatggccg cgatagcggg
      781 ccgggatgaa ctcctgaatg gtggaggcga tcgccgtgta ttcgccgccg atgccggcgc
      841 cggtcaaaaa acggaacagg gcgaagctca cgaagttcca ggagaaggcc gtcgccgccg
      901 tggcgatcag atagacggcc agcgtgatga agaacagctt cttacggccg aggcggtcgg
      961 tgagccatcc aaacagcagt gcccccagca ccgcgccggc cagatagaag ctggaggcaa
     1021 gtccgacctc cgcattcgtc agcttcagga cggggctctc tttcagcgcg ccagccaccg
     1081 cgccggcgag cgtcacttcg agcccgtcca gcacccaggt gatgccgagt gccaagacga
     1141 tgagcgtgtg gaagcgtccc cagcgcaggc gatcgagccg cgccggcacg gacgtctcga
     1201 tcaccttcgc gtcgtgggcc ccgctcacga tttgccggat gctggccgcg gcacgatggg
     1261 aaagtcgaaa taggtgtcgg ggtagggctc cggctcgaac gtgtaatgcc agatctcctt
     1321 gggatagttc ttaaagccgt ggcggctcat cacgtccaca aggagcgtcc ggttgcgctg
     1381 ctgctccggc gtgagaccgg gcgcatccgt gttggccttc ggatcgaagc agtcgaagcc
     1441 ggttcccatg tcgacgctgt tgtcggacgg ctgcgcctca gtgcaggcga tgccgtcctc
     1501 gtcgtccgtg tcggcaggtg acgatccgag gggcacgatg gtgacatcca gcgtcgaccc
     1561 gcgggagtgc ccggaccgcg tggcgatata ttcgggaaac agggcgctct tgtgcaggtc
     1621 gggataatgc tcgagcttgg cgttcggatt gtccggctct ttcgaccaat cgacgaaggc
     1681 ggtcacggcc tgtttggggc ggtagcagtc atagaccttc agcgacaacc ccattggctt
     1741 gaggtccgtt tgcgcggctt tcaaggcttc ggccgcttcc ctgacaagca cgcattcggc
     1801 ggcgtcgtag ccgggcacgg gctgccgcgt gaagttattg gggcctgcat agcgcatgtc
     1861 ctgctcgatc gaagggtcga cgtcgtagag atagacgaag cccttgggca tggaaccagc
     1921 gctcatgggt gacgtccccg caacaaagag agctgcaacc agcacggcgc agccgatagc
     1981 tcgacaacca gacatggggt gactgtttgc gatggggttt gcgccgtcaa cgcatggcgc
     2041 aaaaaaactc cccgctggag ggcggggagt ggaagtgttt gggggactga catgactggc
     2101 agggccagtc ggtgtcggac cattagcagg gaactgccgg cgctcttacg ataaggacgg
     2161 ttggctctac cggcgtcgct ccagccgcct gcccatcagg gctaccgtaa gatcgttcct
     2221 attatttacc gcaatcgacg ccctatctca agcaaaaccg cctgtgacat ttccgcaaca
     2281 gtcaaccgga atcgcactaa attcttctag ggcttgggat attccggcct gccgggggtc
     2341 tcggtcttgg cgataatgac gtcgtcatgg ggcggaccca gaggctgggg ctgcggctgc
     2401 gggcgccatc tgattcccgt ctccaccacg acatccaccg gaccgccgat catgacgaga
     2461 tgctcgacgt cgtcgcggcg taagagatag agcgtacgct tggagtcgac ctcggccgtc
     2521 tcgacgacgg tgaggcgctt ttcaggcgcc cggcgtgtcg aaccgcccga cttgccgaag
     2581 aatgtcttga acgcccaaac aagaagggcg atgagcgcga cggcaaagag aagcgttgcg
     2641 acgccgagaa tgagggtgtt gatctccatg atggatggtc cctacccagg cagagcttaa
     2701 cagttcttta gccgagaggc tccccccttg aaacgaggcc ccggaaacgg ggctgaaccc
     2761 ggactaacct tgcggtcatc cgtccatgac aatctccggc caagacaagt cgaatcgggt
     2821 attttcccaa gccatgacgg atattaacgc gccaatgcgc tacgccgagc ccgttgctga
     2881 ccagaccgag cgcgacggga gcattagcct ggtcgtactc ctcgcggtcg cgcttgcggt
     2941 agccgccgtg gggctggcca tggtgagccg ggagatggcc gagccgttcg tgctggcgat
     3001 cctcgccggc cttgcggttg tcggcgtgtt ctgcctgttc gcgggcgcgg tcggcatttt
     3061 gtatttcggc cagcgccacg cgcggaacga cctgaccaag gctttcgtcg acaacctgcc
     3121 gcaaggcgcg ctgatcgccg acgcgacagg gcgggtgctt tacgccaacc gggcctatcg
     3181 cgaccttctg gggctcgacg gcgaggcaag cgtcttggcg ccggaccgag ccttcggtgg
     3241 caacccgcat ttggccgagc cgattttccg cctggcccgt gcggctcaac aaggacgccg
     3301 cctgaaggaa gagttccgcc tgccgccgcc gggcgaagtt caagacgagg gcggtatcac
     3361 cccgcgctgg ttccgcatct cggtgcagcc aatgccgcca gatccgaagg caggccgcaa
     3421 aggggcgctc gttgtctggc aggtagacga gatcacccaa gaccgcgccc gcgaggaaac
     3481 cagcttcgcc aaagtgcaag cggccatcga ctatctcgac aacgccccgg ccgggttctt
     3541 cattgccgac gcggacggcc atatcgacta tctgaacgcc accttgtcgc aatggctcgg
     3601 ccttgatctg tccgaggttt ccaaccgcca gatcaagctc acgcagatca tgtccgagga
     3661 cagcgccgcg ctgatcgccg gcgccagccg cggcgacacg caaggcggca cccgccattt
     3721 cgatatcgac ctgctcaaag ccaacggcac atcgctgccg gtgcgccttc tcttacgcct
     3781 gccgcgcggc agcgaactgg cgcatgtgct gatcctcaat cgcgggcccg gtgaagtgga
     3841 ggaggccggc gccggcgagc tcagatttgc gcggctgttc cattcctcgc ccattgcgat
     3901 tgcaacggtg gatacgacag gcacgttgag cgcgacaaac gcggccttcg tgcgcatgtt
     3961 tggcgccgag accggcgatg gcgagacgag cctggagagc tttctcgatc cgtccaacca
     4021 cgccgatctt cgaaaagctc ttgaatccgc aattgccggc caggttttga c
//
LOCUS       DBNHVU010000033        12969 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_755677, whole genome shotgun sequence.
ACCESSION   DBNHVU010000033 DBNHVU010000000
VERSION     DBNHVU010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 12969)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12969)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12969
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_755677"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="AC16Y7_01250"
     CDS             complement(<1..888)
                     /locus_tag="AC16Y7_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007066472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HIR5708035.1"
                     /translation="MTVFPQLEIPETLAAGPGPGNTDPRVLERFAKTGVADHMQADVL
                     RGMKEAKQMLRDLWGTKNVYTFGVAGTGWSGLDCVHSLILPGDKVVTFANGTFSGIDD
                     LTIRIKASSLEDLAADSVNPKPKNVVTVTVPHGQSVSGETVEKALAEHKPMWAFMAHW
                     ETGSGRVNDLKGFSDACVKHGVMGIVDAVSSLGVGDFSIDDYPGVVAWASCPQKGVLS
                     LPLTYAPVSFTDKAIEVVKKRGCYTFVNHPILDARHWGIIDGKDVETPVYHQTHSCYA
                     VAAFHEALRILLSYGRAAKA"
     gene            complement(1009..3057)
                     /locus_tag="AC16Y7_01255"
     CDS             complement(1009..3057)
                     /locus_tag="AC16Y7_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR5708036.1"
                     /translation="MGQAQGQPARSARCIALVGPYLSGKTTLLESILARTGAINRQGS
                     VAAKNTAGDASDEARHHGMSVELNAATIDFLDDTFTFLDCPGSIEFQADSANVLAACD
                     AAVVVCEPDEKKVPALQLILKQLEDRGIPHFLFLNKIDKAEARVRDILPMLQPASSVP
                     LVLRQVPIWEKEIVTGFIDLALERAHVYREHAPSEVVEIPDAVSEREQEARFSMLEQL
                     ADYDDELMEQLLEDVPPPRDKVFDDLATDLREGFICPVLLGSAENGNGILRLLKALRH
                     EAPFVAATAERVGAEGAKSCAFVMKTFNTAHAGKLSLTRVLTGEFADGDSVYGGESEE
                     RITGVFNLLGHEPVKRGPAKPGDTVAFGRLESVKTGDTLLEEKGASLKIEPGTITQPV
                     FGLAVEAREKKDDVKLTTGLAKIIEDDPSLSITHDQDLGQMVLWGQGEMHLRVALERL
                     IRKYGVGAATLPRQIPYKETIRKSTEVRGRHKKQSGGHGQFGDVVVTIKPLARGEGIA
                     FSETITGGVVPKNYIPSVEHGVRDYLHAGPLGFTVVDVAVCLIDGSYHSVDSSDMAFR
                     QAGRLAMSEGMPQCQPVLLEPIMAVEIAVPSEATARINSIVSSHRGQILGFDARPDWP
                     GWDVVQAHIPEAEIQNLIVELRSATSGVGTFQAKPDHLAELVGKAADMVLAHRGDKAA
                     "
     gene            3249..3773
                     /locus_tag="AC16Y7_01260"
     CDS             3249..3773
                     /locus_tag="AC16Y7_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708037.1"
                     /translation="MRFVFCLGLLALLLVALAPAGGDHASAFTPMAPSTPTSIVLDAK
                     IVCGDFGQGYTCRRESGAIRRGKMRKVPSSRSSSGSTSSGGGWFGGSDDEDAPPPPDA
                     AAGASGGAPASGACPPNSERLGGHCIPYTQSCKRGLAANAPPQVCRGAEEKLVCDFRA
                     DGLKDCCCRTYNKF"
     gene            complement(3809..4717)
                     /locus_tag="AC16Y7_01265"
     CDS             complement(3809..4717)
                     /locus_tag="AC16Y7_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5708038.1"
                     /translation="MDTRVTDDSPIATGRSSRVHRLADGRRIGFAEYGDPDGLPAIAL
                     HGTPGSRDMFALTDAGARARGLRIIAPERPGYGLSEAHHFDALPQTASDVEAIADALG
                     LDRFALIGVSGGAPHAIAAVSLLKDRILRLLLIGPVGPIADCDHIRMSHLHQFIFTRM
                     APSPHAAGAFFMGLRTLIEWAPGAAYHLLLQRVPETDRQVLYRPEVRENLQTAMKEGL
                     RPGVEGPLQDLRLYCGAWNLPLGEVAVPTVMWQGSEDTVVPPDAAYFLAGELPNCQLE
                     IIEGAGHYWVFGEFGHVLDAVRAAWS"
     gene            complement(4772..4990)
                     /locus_tag="AC16Y7_01270"
     CDS             complement(4772..4990)
                     /locus_tag="AC16Y7_01270"
                     /inference="COORDINATES: protein motif:HMM:NF015424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HIR5708039.1"
                     /translation="MKLSKLSTANQIEGKIMEIRKGAVTAIVYVQIAGDSMVEVSVTI
                     ASLETLGLFEGKTVALIINPGDIMIGCD"
     gene            complement(5030..6232)
                     /locus_tag="AC16Y7_01275"
     CDS             complement(5030..6232)
                     /locus_tag="AC16Y7_01275"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR5708040.1"
                     /translation="MGLVSRTLERVKPSPTMAITSKAREMKAAGFDVIGLGAGEPDFD
                     TPDNIKRAAIEAIQRGETKYTAVDGIPELKQAISEKFARENGLDYQLTEITVGSGGKH
                     VLYNALLATLDPGDEVVIPSPYWVSYPDIVLLAGAEPVVVQTKLEDGFKLTPEALEKA
                     ITPKTKWFIFNSPSNPTGSAYTRDEIKALTDVLLRRERVWVLSDDIYEHLVYDGFKFS
                     TPAQVEPKLKDRALTLNGVSKAYSMTGWRIGYAGGPAPLIKAIGKLQSQSTSNPCSIS
                     QWAAVEALRGPQDFLGDWVASFKERRDLVVSMLNQANGLSCATPEGAFYVYPSCEGCI
                     GKKTPKGDVVADDEAFATLLLQEEGVAVVHGAAFGLSPFFRVSYATGIEALEEACNRI
                     QRFCASLR"
     gene            6981..7562
                     /locus_tag="AC16Y7_01280"
     CDS             6981..7562
                     /locus_tag="AC16Y7_01280"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase family protein"
                     /protein_id="HIR5708041.1"
                     /translation="MLLAAAAQFQHALPPIGEDGFVQALRQSVWKRVLADQTHATPWP
                     WQDLSTNMSRAPGATVPRLGLSAALRDHVVSEPASPAPDGRTTKTVSNAPPRDHAAQG
                     REQQGDVALSDVTIGDSITFTGTDGATCVYQVTGRRVVDPHLAASEAERFDGEASLFE
                     CSPLESLIMQATQGAAQVEPTAPVAPGGDQQKL"
     gene            complement(7572..8252)
                     /locus_tag="AC16Y7_01285"
     CDS             complement(7572..8252)
                     /locus_tag="AC16Y7_01285"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5708042.1"
                     /translation="MGPTMRLILGLSALVLILAAVALGLPAHVTVARSVVINAPEYAV
                     YPYLNNLRRFQEWSPWVARDPEMKFTYSGPREGKGAKVEWVSGKPSIGTGSMQIVQTE
                     PSRSVDLTANINGLEGDSSYELEPSGSGSKVTWSFGYQTGSSPFKRWKALMLDGFIGA
                     EYRAGLEKLKALVEGDRAPAVRRVVPPTPTAPPAAPPAAGGETVVPQQTPAATPAAEA
                     APPPPPPQ"
     gene            8387..8557
                     /locus_tag="AC16Y7_01290"
     CDS             8387..8557
                     /locus_tag="AC16Y7_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708043.1"
                     /translation="MSLSAPTTIVFIVSAILAALAVIGTFMPIPFITENAFWVAVVAY
                     VILACGNIFRGF"
     gene            complement(9671..10453)
                     /locus_tag="AC16Y7_01295"
     CDS             complement(9671..10453)
                     /locus_tag="AC16Y7_01295"
                     /inference="COORDINATES: protein motif:HMM:NF025389.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin-like domain"
                     /protein_id="HIR5708044.1"
                     /translation="MATILSLMHGTHLWVVALIMMVVAEVYAVGLMLMSRYRYGADRL
                     KLNNEVAGFKFAVVGVFYAVMLAFVVIAVWENYSETENAVREEAKAAVDLYHVAEAIP
                     NQGDDIQAGVMRYVKDVQTHAWDSMAVGERNEVVARDLRALNSAVLKLQPEDDKELVL
                     FQHALTLVTLIADSRNERLDSASGSVPGVLWFVLIVGGLITLGYPAFFAASNAGAQVL
                     MTVALAALVALSLLLALAFDFPFTGNPHISKEPFAEVLLQME"
     gene            10545..11393
                     /locus_tag="AC16Y7_01300"
     CDS             10545..11393
                     /locus_tag="AC16Y7_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008189079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HIR5708045.1"
                     /translation="MTKQGASTKTPQTQEGLTRHETKEVKERRRLRAAVVYEIIRLEG
                     EAELARTFTALWWSALAAGLSIGFSVLSQALLEATLGDFPGRTVIADFGYCVGFLIVI
                     LARQQLFTENTLTAVLPVMVRKDWSGIWDVLRLWGIVLAGNLVGCFVFAAFLAYSGAF
                     SDQTQTAITEIGRHLMANSPTEMFVKGIMAGWLIAALVWMLPSSEGTEIFIITLITYI
                     IALGDFTHIIAGSVEAIYMCLIGEVGIGQAVGGFFIPTLLGNVAGGTVLFALISYAQV
                     REELEG"
     gene            11401..11823
                     /locus_tag="AC16Y7_01305"
     CDS             11401..11823
                     /locus_tag="AC16Y7_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HIR5708046.1"
                     /translation="MTVPLWVLLTLAVWTLLVLLAGVGLHRWSLILSSKAELTDFPGG
                     VAEGAPAYCRAVRAHANCVENLPVYGAIAITAAIAGVDTPRLDQLAIVLLIARIGQTL
                     IHMIFRETNRTVAIRFGFFAVQLLAMFWMAAIVAMTAW"
     gene            11876..>12969
                     /locus_tag="AC16Y7_01310"
     CDS             11876..>12969
                     /locus_tag="AC16Y7_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HIR5708047.1"
                     /translation="MPPRAPKTFGSRAEPRAVTGEAAGLPLAGDRRDPGFGEAPQPLI
                     QEHPLVTGETAAFTPHRPPRPDKSEGGIALQTETELEPKGDQPQAIAELVAQVNAHER
                     DQVLLGVTGSGKTFTMAKVIEETQRPALILAPNKTLAAQLYGEFKSFFPENAVEYFVS
                     YYDYYQPEAYIPRTDTYIEKDSSINEQIDRMRHSATRALLERDDVIIVASVSCIYGIG
                     SVETYTAMTFTLKRGDQIEQRQLMADLVALQYRRNDVNFVRGSFRVRGDTIELWPAHL
                     DDRAWRVSLFGNEIESIVEFDPLTGAKTDEFSLVKVYANSHYVTPKPTLKQAVRGIKE
                     ELKQRLTELHGAGRLLEAQRLEQRTLFDLE"
BASE COUNT         2317 a         4304 c         3910 g         2438 t
ORIGIN      
        1 cgccttggcg gcacggccat aggacagcag gatgcgcagc gcctcgtgaa acgccgccac
       61 ggcgtagcag gaatgggtct ggtgatagac cggcgtttcg acatccttgc cgtcgatgat
      121 gccccaatgg cgcgcatcga ggatcggatg gttcacgaag gtgtagcagc cgcgcttctt
      181 gacgacctcg atggccttgt cggtgaagct gaccggcgcg taggtcagcg gcagcgacag
      241 cacacccttc tgcggacacg acgcccaggc caccacgccc ggatagtcgt cgatgctgaa
      301 gtcgcccacg ccgaggctcg acacggcgtc gacgatgccc atgaccccat gcttcacgca
      361 ggcgtcggaa aagcctttga ggtcgttgac gcggcccgag ccggtctccc aatgggccat
      421 gaacgcccac ataggcttgt gctcggccag cgccttttcc acagtctcac cggacaccga
      481 ctggccatgc ggcacggtca cggtgacgac gttcttcggc ttcgggttga cggagtcggc
      541 agcgagatcc tcaagggacg aggccttgat gcggatggtg aggtcgtcaa tgcccgagaa
      601 cgtgccgttg gcgaaggtca cgaccttgtc gcccggcagg atcaggctgt gaacgcagtc
      661 gaggccgctc cagccggtgc cggccacacc gaacgtgtag acgttcttgg tgccccacag
      721 atcgcgcagc atctgcttgg cttccttcat accgcgcagc acgtccgcct gcatgtggtc
      781 ggcgacgccg gtcttggcga accgctcaag cacgcgcgga tcggtgttgc cgggccccgg
      841 gccggcggca agcgtctcgg ggatttcgag ctgcggaaat acggtcatgg gaacaccctc
      901 tatctaactg gtcgaaaagc taggaattcg gcctaatcgt ctaccggccc aggcggggcg
      961 gggcaaggcc cccgcgccaa ttttggcccg ccctggccct gccgtccgtc aggccgcctt
     1021 gtccccgcga tgggccaaga ccatatccgc agccttgccg acgagctcgg cgaggtgatc
     1081 cggcttagcc tggaaggtcc ccaccccgga tgtcgccgaa cgcagctcca cgatcaggtt
     1141 ctggatctcg gcctccggaa tatgggcctg gaccacatcc cagccgggcc agtcgggccg
     1201 cgcatcgaag cctagaatct gcccccggtg ggacgagacg atggaattga tgcgtgccgt
     1261 ggcctccgac ggaacagcga tctcgacggc catgatcggc tccaatagaa ccggctgaca
     1321 ctgcggcatg ccctcggaca tcgcgagccg ccctgcctga cggaaggcca tgtccgacga
     1381 atcgaccgag tgataagacc cgtcgatcag gcatacggcg acatccacca ccgtaaaccc
     1441 gagcggtccg gcgtggagat agtcccgcac gccatgctcg accgacggga tgtagttctt
     1501 cggcacaaca ccgccggtga tggtttcgga gaaggctatc ccctcgccgc gcgccaaggg
     1561 cttgatggtc accaccacat cgccaaactg gccatgaccg ccggactgct tcttgtggcg
     1621 tccacggacc tcggtcgatt tgcggatggt ttccttgtag ggaatttgcc gcggcagggt
     1681 tgccgcgccc acaccgtact tgcggatcag ccgctccagg gccacacgca gatgcatctc
     1741 cccttgcccc cacaggacca tctgacccag gtcctgatcg tgggtgatcg agagcgaagg
     1801 gtcatcctcg ataatcttgg ccagcccggt cgtcagcttc acgtcgtcct tcttctcccg
     1861 ggcttcaact gcgagcccaa acacgggctg cgtgatcgtg cccggctcaa tcttgagtga
     1921 cgcgcctttc tcctctaaca gcgtgtcgcc ggtcttgacg ctctccaatc ggccgaacgc
     1981 gacggtatca ccgggtttcg cagggccacg ctttactggc tcgtgtccca ggagattgaa
     2041 cacgccggtg atccgttcct cgctctcgcc gccatagacg gagtcgccat cagcgaactc
     2101 acccgtaaga acccgggtga gcgacagctt gccggcatgg gccgtgttga aggtcttcat
     2161 cacgaatgcg caggatttgg cgccttccgc gccgacccgc tccgccgtcg ccgccacgaa
     2221 aggcgcttca tgacggagcg ccttcagaag ccgcaatatg ccgttgccgt tctctgccga
     2281 tcccagcagg accgggcaga tgaaaccttc gcgcagatcc gtggccagat cgtcgaagac
     2341 cttgtcgcgc ggcggcggca cgtcttcgag aagctgctcc atcagttcgt cgtcatagtc
     2401 ggcgagctgc tccagcatcg aaaagcgcgc ctcttgctcg cgctcggaca cggcatcggg
     2461 aatctccacc acctcggacg gcgcgtgctc gcgatagacg tgcgcgcgtt ccagcgcgag
     2521 gtcgatgaag cctgtgacga tctccttctc ccagatcgga acctggcgca gcacgagcgg
     2581 cacgctcgag gcgggctgca acatcggtag gatgtcgcgc acgcgcgcct ccgccttgtc
     2641 gatcttattg agaaataaga aatgcggaat gcctcgatcc tccagctgtt tgaggatcag
     2701 ctgaagcgca ggcaccttct tctcgtcggg ctcgcacacc accacggcgg cgtcgcacgc
     2761 ggccagcaca ttcgctgagt ccgcctggaa ctcgatggag ccgggacagt cgaggaaggt
     2821 gaatgtgtcg tcgagaaaat cgatcgtggc cgcgttgagc tcgacgctca tgccgtgatg
     2881 gcgggcttcg tctgaggcat cgcccgccgt gttcttggcg gcgacactgc cttgcctgtt
     2941 gatggcgccc gtccgcgcca gaatagattc gagaagtgtt gtcttaccgc tgagataggg
     3001 gccaaccagt gcgatgcagc gggcgctgcg ggctggttgg ccctgcgctt ggcccatgct
     3061 ctcctccctt tgcctgctcg caaaagtgga ccttttcggc ccttttttcc ttccgcgagc
     3121 cttgaggaca ctatcgtgtc aagggacagg cactctggca agagccgcga gtcgccaaaa
     3181 ttgctgcagc acttctgatc ccgtgacgga gccagtaagg tggcgcctcg tcacgaacgg
     3241 ggagttccat gcgtttcgtc ttctgtcttg ggttgttggc tttgcttttg gtggcgcttg
     3301 cgcctgccgg cggcgaccac gcgtcggcct ttacgccgat ggcgccgtca acgcccacat
     3361 cgatcgtcct cgatgccaag attgtctgcg gcgactttgg tcaaggctac acctgccgga
     3421 gagaatccgg cgccatccgc cgcggcaaga tgcggaaggt tccgagctcc cgatcgagca
     3481 gcggctcaac cagttccggc gggggatggt tcggcggcag cgacgacgag gatgctccgc
     3541 cgcctcccga tgctgccgca ggcgcaagcg gtggcgctcc ggcatcgggc gcttgcccgc
     3601 ccaactccga acgtctcggc ggacattgca ttccctatac ccagtcctgc aaacgcggcc
     3661 ttgccgcgaa cgcgccgccg caagtctgcc ggggcgctga agagaaactg gtttgcgact
     3721 tccgcgccga cggcctgaag gattgctgtt gccggaccta caataagttc taagcgcgtg
     3781 cgctattcgt tggcatgcgg gcccaagctc aagaccacgc cgctctaacg gcgtccagca
     3841 cgtggccaaa ttcgccaaag acccagtagt ggcccgcgcc ctcgatgatc tcgagctggc
     3901 aattggggag ttcaccggcc aagaagtagg ccgcgtccgg cggtacgacc gtatcctcac
     3961 tgccttgcca catgacggtc ggaacggcga cctcgccgag cggcaagttc cacgccccac
     4021 aatagagccg caagtcctgg agcggacctt cgacacccgg tcgcaagcct tccttcatcg
     4081 ccgtctggag attttccctg acctccggtc gatacaacac ctgccggtcc gtctccggca
     4141 cacgttgaag gagcaggtga taagcggccc ccggcgccca ctcgatcagc gtcctcagtc
     4201 ccatgaagaa ggcgcccgcc gcatgcggcg acggcgccat gcgcgtgaaa atgaactgat
     4261 gcagatgcga catgcggatg tgatcgcaat cggcgatcgg cccaaccggc ccaatcagca
     4321 gaagccgcaa gatccggtcc ttcagtaggg acaccgccgc gatcgcgtgg ggggccccac
     4381 ccgagacgcc gatcagggcg aagcgatcga gcccgagcgc atcggcgatg gcctccacgt
     4441 ctgaggccgt ctgcggcagc gcgtcaaagt gatgggcctc ggacaggccg tagcccggac
     4501 gctccggcgc gatgatgcgc aagcccctcg cccgcgcacc ggcgtcggtc agcgcgaaca
     4561 tgtcacgaga gcccggcgtg ccatggaggg caatcgccgg caagccgtcc ggatcaccat
     4621 actcggcaaa gccgatcctc cgcccatcgg cgaggcggtg cacccgcgag gagcggccgg
     4681 tagctatcgg gctatcgtct gtaacgcgcg tatccatgga tcctcgaggg cgcgctggtg
     4741 ggccccgccc ccaaggccag gctcaattgc gctaatcgca gccgatcatg atgtcgccgg
     4801 gattgatgat cagggccacc gtcttgcctt cgaacagccc gagggtctcg agcgaggcaa
     4861 tggtgacgct cacctcgacc atagagtcgc cggcgatttg cacatagacg atggctgtca
     4921 cggcaccctt gcggatttcc atgatcttgc cttcgatctg gttggccgtg ctgagcttcg
     4981 aaagtttcat ggcctcaccc tccgctgatt cgacgccagt atagacctgc taacgaaggc
     5041 tcgcgcaaaa gcgctggatg cggttgcagg cctcttccaa agcctcgatg ccagttgcat
     5101 aggacacccg gaagaacggc gacaacccga acgcagcgcc gtggaccacg gccacgccct
     5161 cttcctgcag caacagggtc gcgaaggcct cgtcgtcagc gacgacgtcg cccttcggcg
     5221 tcttcttgcc aatgcatcct tcgcaggacg ggtagacgta gaatgcgcct tccggcgtgg
     5281 cacaactcaa gccattcgcc tggttcagca tcgaaacgac gaggtcgcgc cgctccttga
     5341 acgacgccac ccagtccccc aagaagtctt gcggaccgcg cagcgcttcg accgccgccc
     5401 actggctgat cgagcacggg ttcgacgtcg actgcgattg cagcttgccg atcgccttga
     5461 tgagcggtgc cggtccgccg gcatagccga tacgccaacc ggtcatcgaa taggccttcg
     5521 acacgccgtt cagcgtcagc gcccggtctt tcagtttggg ctcgacttgc gccggcgtag
     5581 aaaatttgaa gccgtcatag accaggtgct cgtagatatc gtcggacagc acccagaccc
     5641 gctcgcggcg aagcagcacg tcggtcagtg ctttgatctc atcgcgcgta taggccgacc
     5701 ctgtcgggtt ggacggcgag ttgaagatga accacttcgt cttcggcgtg atcgccttct
     5761 cgagcgcttc cggggtcagc ttgaacccgt cctcgagctt ggtctggacc acgaccggct
     5821 ccgcgcctgc cagcagaacg atatcgggat agctcaccca atagggcgac gggatgacga
     5881 cctcgtcgcc cgggtccagc gtcgccagca gcgcgttgta caagacgtgc ttgccgcccg
     5941 agcccacggt gatctcggtt agctggtagt cgagcccgtt ctcgcgcgcg aacttctccg
     6001 aaatcgcctg cttcagctcc gggatgccgt ccacggcggt gtatttggtc tcgccgcgct
     6061 ggatggcctc gatcgccgcc cgtttgatgt tgtcgggcgt atcgaaatcg ggttcgccag
     6121 cgccaagacc aatcacgtcg aacccggcgg ccttcatttc gcgcgccttg ctcgtgatgg
     6181 ccatggtggg agacggcttg acccgttcaa gcgtgcggga gaccagtccc atgaagaact
     6241 cctctaaggt cgaaagatgc gggaaaaatt cgaggcaacc ctattcgggc gcgcatatcc
     6301 tcgcaagtgc aatgcacaat atgacagtgc aaaaaaggcc gcagcgcggg ataagcgcgc
     6361 cccttgcggc tttcccaagc agaacagcca gcgtccacgc gagacgcgtg ccacacatgt
     6421 ggcgggaagc tccgcctcac agccacatta tggttaagcc tccgccccat tgcggtgctc
     6481 aactgacgcc cgttgccgaa catcgagggc tcatcgacga tgctgcgccg ccgcgttccg
     6541 cgcccctccc agctcccagc agcctggccg cttccagcgc tgatggcggc cgtaatcctt
     6601 gccgtttcaa gctgtcccgc gccggtcaaa gccgcagaaa tagctgaatc cagggcggat
     6661 agcggtgttt ttaccagatt tgagcaggcc ctgaagcttc ccgccgggcg gggctatgat
     6721 aatgtcacga ctcaggtcga aagcgctgag ccgacggccc caaacggcaa gacagggcaa
     6781 atcacgatgt tcgtccttct attcaccttc cttagcgcgg tgacgctgct catgtggcgt
     6841 cactcccgcc gcgacaatgc ctccccgcgg cggatcggga ggaggatctg atgccgttac
     6901 gggggtcggt acagctcctc ctcctttccc ctcgccggcg cgtcgcgcgc cgggccgggg
     6961 ttggaacggc tgttgcggtt ttgctacttg ccgcggcggc ccagttccag cacgcgctcc
     7021 cgccgatcgg cgaggatggg tttgtccagg cccttcgcca gagcgtctgg aagcgggtgc
     7081 ttgcggacca gacccacgcc acgccttggc cctggcagga cctctcgacg aatatgtcgc
     7141 gcgcgccagg ggcgacggtg ccccggctcg gcctgagcgc cgccttgcgc gatcatgtcg
     7201 tttccgagcc cgcctcaccg gcgccggacg gccgcaccac caagactgtt tcgaacgcac
     7261 cgccgcggga ccacgcagcc caaggtcgag aacagcaagg cgacgtggca ctgagcgacg
     7321 tcaccatcgg cgacagcatc accttcaccg ggaccgacgg cgccacgtgc gtctatcaag
     7381 tcaccggccg ccgcgttgtg gacccgcacc ttgccgcgag cgaggcagag cgtttcgacg
     7441 gcgaagcctc actgtttgag tgcagcccgc tggaaagcct gatcatgcag gcaacgcagg
     7501 gcgcggccca agtcgagcca acagctcctg tcgccccagg cggcgaccag caaaaactct
     7561 aagacaaacg tctactgagg gggcggtggc ggaggcgcgg cttccgcggc cggtgtagct
     7621 gcgggcgtct gctgcggcac caccgtttca cccccagcgg caggcggtgc agcgggcggc
     7681 gcggttgggg tcggcggcac gacccttcgg accgcaggag cccggtcgcc ttccaccagg
     7741 gccttcagct tctccaagcc ggcgcgatac tccgcaccga tgaagccgtc aagcatcaag
     7801 gccttccaac gcttgaaggg gctagaaccc gtctgatagc cgaagctcca ggtcaccttg
     7861 ctcccggagc cggacggctc gagctcgtag ctactgtcgc cttcgagccc attgatgttg
     7921 gcggtgagat ccacgctgcg ggacggctcg gtctgcacga tctgcatgct gccggtgccg
     7981 atggatggct taccactcac ccactcgact ttcgcaccct tcccttcccg tgggccggaa
     8041 taggtgaact tcatttccgg atcgcgggcc acccacggcg accactcctg gaaccggcgc
     8101 aagttattca gataaggata gaccgcatat tccggtgcgt tgatgacgac cgatcgcgcg
     8161 acggtcacat gtgccggcaa ccccagggcc acggccgcca gaatgagtac cagagcgcta
     8221 agccccagga tcagccgcat tgttggcccc atcacacaag tctcctcatc gttcggcgta
     8281 gagtcccccg aaaggcagac agccagccct gttggggtcg gccaacgccc tcgctttttc
     8341 acagaaaacg attaaagtcc gctcgtcaac aagagggagg gtagagatga gtctgtcagc
     8401 accgacgacc atcgtcttca tcgtttcggc tattctggcg gcattggccg tcatcggcac
     8461 gttcatgccg attccgttca tcaccgaaaa tgcgttctgg gtcgccgtag tcgcctacgt
     8521 gatcctggca tgcggcaata ttttcagagg tttctagcct caagagcttg aggtacgtgg
     8581 aaagaggaag ggccggctga aaagccggcc cttctgtcgt ctagtcgtcg tcgtcccggg
     8641 cccacgatgg ccgatagggc atcggggtcg gccgcggtcc cgccggcgca cctggcatcg
     8701 gcggacgcgg cgtaccgaat ccgggcgaac cgcccgtcgg cgttgccggg ctgccaacac
     8761 tgggcgacgg tggggtcacc ggacgcggtg ttggggcgac cgggctcacc ggcgcagcag
     8821 cgaccggctt tggcctgacc accggcttgg ccgccggttt gcgcctgacg gcctttttgg
     8881 ccttcgctgc ggacttggtc gccggcttgc gagccggcgc cgatcgcgca gacttcttgg
     8941 ccgccgactt tcgagccgtc ttctttcgag ccgcagtctt ccgtcgagtg gtcttccgcg
     9001 cggtccgtgc cgtcttgcgg gcggcggtct tccgccgcgc ggtcgtcttg cgagcagtct
     9061 tccgcacttt ccgtgctgtc ttgcgcgccg tggtcttccg ccgaacgccg gtcttgcgag
     9121 cggtcctccg cactttccgt gctgtcttgc gcgccgcggt cttccgccga acgccggtct
     9181 tgcgagcggt cttccgcact ttccgcgcag tcttgcgcgc cgcggtcttc cgccgagcgg
     9241 tcgtcttgcg agcggtcttc cgcactttcc gtgctgtctt gcgcgccgcg gtcttccgtc
     9301 gatcggtcgt cttgcgagcg gtcttccgca ctttccgcgc agtcttgcga ccggtcttcc
     9361 gcgcggcccg tgccgtctta cgtccagttg atctcgtggc catagcttcg ctccgattct
     9421 gttttagatg tcgttacccg ttcaaacgac gcatacatcg aatcacgcta cacttggtcc
     9481 aagcttgccg tcccacatgg caagtcaatt gtttttttta gtatacgctg attgcatcag
     9541 aaaatggcaa attaacgggc actattcgct taacgtcaac aaccaattct ttccgcgcgc
     9601 aacgcgatgc gcagcaaact gtacattgcg cgtgcaaaac gattcgtttg cgaagtgcgt
     9661 ttgccgcact tcattccatc tgcaggagca cttcagcgaa cggttccttc gaaatgtgag
     9721 ggttcccggt gaacggaaag tcgaaggcaa gcgccaagag caaagacaac gctacaagcg
     9781 ctgcgagggc cactgtcata aggacttgcg cgccggcatt tgacgcggcg aagaacgccg
     9841 gatagccgag ggtgatcagg ccgcccacga tcaacacaaa ccagagcacc cctggtaccg
     9901 acccacttgc gctgtcgagc cgctcgtttc ggctgtcggc aatcaaggtc accaaggtga
     9961 gcgcgtgctg gaacagcacc agttccttat cgtcttccgg ctgcagtttg agaacggcac
    10021 tgttcagagc cctgaggtcc ctggccacga cctcgttacg ctcgccaacc gccatgctat
    10081 cccaagcgtg cgtctgcacg tcttttacgt acctcatgac ccccgcctgg atatcgtcac
    10141 cctgattggg tatcgcctcc gccacgtggt agagatcgac ggcggcctta gcctcctccc
    10201 ggacggcatt ttccgtttcg ctgtagttct cccaaacggc gatgacgacg aaagccaaca
    10261 tgacggcgta gaacacgccc accacggcga atttgaaccc ggcgacttca ttgttgagct
    10321 tgaggcggtc ggcgccgtaa cgatatcggc tcatgagcat gagcccgacc gcatagacct
    10381 ccgccaccac catcatgatg agggccacga cccacaaatg cgtcccgtgc atcaaagaga
    10441 gaatggtggc catggtcttc cgtcccggga aagcgaccga taggaacgcc gccttaggcc
    10501 cgctattttg ggaccaacat gagcgattcg cgacaaggaa gcccatgacc aagcaaggcg
    10561 caagcacaaa gaccccccaa acgcaggagg ggctgacgcg gcatgaaacc aaggaagtca
    10621 aggaacgccg tcgtctgcgc gctgcggttg tctacgagat catccgcttg gagggtgagg
    10681 cggagcttgc gcgcacattc accgcgctgt ggtggtcggc cctggcggcc ggtctttcga
    10741 tcggcttctc cgttctgtcc caagctctgc tcgaagcgac tttgggcgac ttcccgggcc
    10801 ggaccgtgat tgccgatttc ggctactgcg tcggcttctt gatcgtgatc ctcgcccgtc
    10861 agcagctctt caccgagaac acgctgactg ccgtcttgcc cgtcatggtc cgcaaggatt
    10921 ggagcggcat ctgggacgtg ttgcgcctct ggggcatcgt cctcgccggc aatctcgtcg
    10981 gctgcttcgt gtttgctgcc ttccttgcct attcgggtgc cttctccgat cagacccaga
    11041 ccgccatcac cgagatcggc cgccacctca tggccaacag cccgaccgag atgttcgtca
    11101 agggcatcat ggcgggctgg ctgatcgccg cgctggtttg gatgcttccg tcctcggaag
    11161 gcaccgagat cttcatcatc accctcatca cctacatcat cgcgctcggc gacttcaccc
    11221 acatcatcgc tgggtccgtc gaggccattt acatgtgcct catcggcgag gtcggcatcg
    11281 ggcaggccgt tggcggcttc ttcatcccca ccctgctcgg caacgtggcc ggtggcaccg
    11341 tactcttcgc cttgatcagc tacgcgcagg tccgagagga actggaaggc taatgaggcg
    11401 atgaccgttc ctctatgggt tctgctgaca ttggccgtgt ggaccctgct cgtgcttctg
    11461 gctggcgtcg gtctccatcg gtggagccta atcctgtcca gcaaagctga gttgaccgac
    11521 ttcccgggcg gcgtggccga gggagcgccc gcgtattgcc gcgccgtgcg ggcccacgcc
    11581 aactgcgtcg agaatctgcc ggtctatggc gcgatcgcta tcaccgccgc gatcgccggg
    11641 gtcgataccc cgcgtctcga tcagctcgcc atcgtccttc tcatcgcgcg gatcggccag
    11701 acgctgatcc atatgatttt ccgcgagacc aaccgcacgg tcgccatccg cttcggcttc
    11761 ttcgccgtcc agctattggc catgttttgg atggcggcga ttgtcgccat gaccgcctgg
    11821 tagcgcggcg gcgcgcctct agccaagccc tctccccgcc gccatattcg cctttatgcc
    11881 ccctcgcgct cccaagacct tcggctcccg ggcggagccg cgcgccgtca ccggcgaggc
    11941 ggccggtctg ccgcttgccg gcgaccggcg tgacccggga ttcggagagg caccccagcc
    12001 gctgattcag gagcacccgc tggttacggg cgagacggcc gccttcacgc cgcaccggcc
    12061 gccacgaccc gacaaatccg aaggcggcat cgctcttcag accgagacgg aattggagcc
    12121 caagggcgac cagccccagg caatcgccga actcgtcgcc caagtgaacg cccatgagcg
    12181 cgatcaggtg ctgctcggcg tcacgggctc gggcaaaacc ttcaccatgg ccaaagtcat
    12241 cgaggaaacc cagcgcccgg cgctcatcct cgcgccgaac aagacgctcg ccgcgcagct
    12301 ctatggggag ttcaagagct tcttcccgga gaacgcggtc gagtatttcg tctcgtacta
    12361 cgactactac cagcccgagg catatatccc gcgcaccgac acctatatcg agaaggactc
    12421 ctccatcaac gagcagatcg accgcatgcg ccactcggcg acacgcgcgc tgctcgagcg
    12481 cgacgacgtg atcatcgtgg catccgtgtc gtgcatctac ggtatcggct cggtggagac
    12541 ctataccgcc atgacgttca cgctgaagcg cggcgaccaa atcgaacagc gccagctgat
    12601 ggctgatctg gtcgcccttc agtaccggcg gaacgacgtc aacttcgtgc ggggaagctt
    12661 ccgggtccgc ggcgatacca tcgaactgtg gcccgcccat ttagacgatc gcgcctggcg
    12721 cgtctcgctg ttcggcaacg agatcgaaag catcgtcgaa ttcgaccccc tgaccggcgc
    12781 caagaccgac gagttcagcc tggtcaaggt ttacgccaat tcgcactatg tgaccccgaa
    12841 gccgacgctg aagcaggccg ttcgcggcat caaggaggag ttgaagcagc gcctcaccga
    12901 gctgcacggc gccggacgcc tgcttgaggc gcagcggctg gaacagcgca ccctgtttga
    12961 tctggagat
//
LOCUS       DBNHVU010000034         2546 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_830327, whole genome shotgun sequence.
ACCESSION   DBNHVU010000034 DBNHVU010000000
VERSION     DBNHVU010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_830327"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="AC16Y7_01315"
     CDS             complement(<1..671)
                     /locus_tag="AC16Y7_01315"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR5708048.1"
                     /translation="MAAALVIGSQAMLFGDQALGQSTKALPKVTATSVFVMDADTGQV
                     LYAKNPDKVFRILSLTKLVTAYILVDQMGDRLSETMTISGAHLAGGSTAGLKQRDVWS
                     LQDLLYGMVLVSGNDASLAIADHVGGNLLAQENKKGDARKRFVAEMRSTAKALGAQQA
                     NFADPYGLARTNVATARDMGLIAARVFTDARLLPAWACTGRILRIEGPKAREVTLKTS
                     LEILG"
     gene            complement(744..1349)
                     /locus_tag="AC16Y7_01320"
     CDS             complement(744..1349)
                     /locus_tag="AC16Y7_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708049.1"
                     /translation="MPVSLFPEGTISTQEISKAEAKVFVISAIALSVSVCNAAFWYGV
                     FGKIFFTHIIYVWAAATAALLASLFIPRMETPLLKLPWRGRFILALPTIWLVLGAFID
                     MESYRPNSIGTWTLWAVTLASAFLTLPYLLYVMIVAVVPDIDRLTHVKLRVALPAIVL
                     LIGVAGFILGTSHTRLLTCENFKIGGHHVPENCRKAGEAPY"
     gene            complement(1366..1896)
                     /locus_tag="AC16Y7_01325"
     CDS             complement(1366..1896)
                     /locus_tag="AC16Y7_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0072570 - ADP-D-ribose binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HIR5708050.1"
                     /translation="MRHPLVFAISKTRYAVITNVHGDILLSNARAIAHGVAPHDHFNQ
                     GLALALREHHPAMAKDFRHYCRQENPKPGHAWLWAGPERVVINLMTQAPAASEKAHPG
                     KATTHNVSHALKELRKILEAEVISSIALPRLATGVGGLGWAEVQPLIESHLGDLPIPV
                     IVYETYEKGVKAAEPT"
     gene            complement(2005..>2546)
                     /locus_tag="AC16Y7_01330"
     CDS             complement(2005..>2546)
                     /locus_tag="AC16Y7_01330"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007068650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HIR5708051.1"
                     /translation="LAAFIERAGGSGKGEITVGTVPDGLEPVYVHEQSRPLSEILSQL
                     LIGSNNYIANQVFLEIGSTLGGPVSLEKSLKVANAFLATNGLAEDIHLEEGSGISRGN
                     HFTARGLAQVLHLFAPHAHLLKGSRGSKYKTGTLSGVRTLAGYAETAKHGRVRFVISL
                     RGNTGRMRYRLLRAIERGL"
BASE COUNT          466 a          759 c          805 g          516 t
ORIGIN      
        1 tcgcctaaga tctccaggct ggtcttcagc gtgacttcgc gcgccttggg tccttcgata
       61 cgcaatatcc gtccggtgca ggcccaggcc ggcagcagcc tcgcgtcggt gaacacgcgc
      121 gcagcgatca ggcccatgtc gcgcgccgtg gcgacattgg tgcgcgcgag gccgtatgga
      181 tcggcaaagt tggcctgctg ggcgccgagc gccttggctg tcgaccgcat ctccgccacg
      241 aagcgcttcc tggcgtcacc cttcttgttt tcctgggcga gcaaattgcc tccgacgtgg
      301 tcggcgatcg cgagtgaggc gtcattgccg gagacgagca ccatgccgta gagcaagtcc
      361 tgcaagctcc agacgtcgcg ctgcttgagt ccggccgtgg agccgccagc caagtgcgct
      421 ccgctgatcg tcatggtctc cgacagccgg tcgcccatct ggtccacaag gatgtaggct
      481 gtgacaagtt tggtcaggct gaggatcctg aagaccttgt ccggattctt ggcgtagagg
      541 acttgtccgg tatctgcatc catcacgaag acggaggttg ccgtcacctt cggtagtgct
      601 ttcgtgcttt ggcccaaggc ttggtctccg aacaacattg cttggcttcc gatcactagg
      661 gcggctgcca tcgcgatggt ggcttgccaa catctcatgc ggcaactgtt gttacgttga
      721 agcacgattt ctccctcgcg gtcctagtag ggcgcctcgc cggccttgcg gcaattttcg
      781 gggacatggt gtcctccgat cttgaagttc tcacaggtca gaagccgcgt gtggctcgtt
      841 ccgaggatga atcccgccac gcctatgagc agcacaatag ccgggagtgc gacccgcagc
      901 ttcacgtgcg ttaacctgtc gatgtccgga acgacagcga cgatcatcac gtagagcagg
      961 tagggaagcg tcaggaaggc gctcgccagc gtgactgccc aaagggtcca tgtgccgatg
     1021 ctgttgggcc gataggactc catgtcgatg aacgcgccca gcacgagcca gatcgtcggc
     1081 agcgccaaaa tgaagcgccc cctccacgga agcttcagca gcggtgtctc catccttggg
     1141 atgaacaggc tcgcaaggag cgctgccgtg gccgcagccc aaacgtagat aatgtgggtg
     1201 aagaagattt tcccgaagac gccgtaccaa aaggcggcgt tgcacacgga gaccgacagt
     1261 gcgatcgccg aaatgacaaa gaccttcgct tccgccttgc tgatctcttg tgtgcttatt
     1321 gttccctccg gaaacaatga tacgggcaat gccgaatttc gggaactagg tgggctcggc
     1381 ggccttcacg cctttctcgt aggtctcgta gacgatgacc ggtataggca agtcgccgag
     1441 atggctttcg atgagcggtt ggacttcggc ccagccaagc ccaccaacgc cggttgcgag
     1501 tctgggcaag gcgattgacg aaatcacttc cgcctcgagg atcttgcgga gttccttcag
     1561 ggcgtgactg acattatgcg tcgtggcctt tccgggatgg gccttctcgc tggccgcggg
     1621 cgcctgggtc atcagattga tgacgacccg ttcgggcccg gcccaaagcc aagcatgacc
     1681 cggtttcggg ttttcctggc ggcagtagtg ccggaagtcc ttcgccatcg ccggatgatg
     1741 ctctcgcagc gccagggcaa ggccttgatt gaaatggtca tgcggagcca caccgtgggc
     1801 gatggcccgc gcgttgctca agagaatgtc accgtgaaca ttggtaatca ccgcgtacct
     1861 cgttttagaa atcgcgaaga ccagggggtg ccgcaccagc gaggccccat ggagtcggca
     1921 ggatacagcg gctcgcgccg ccaccacctt gagcaagctc aagactttgc cggtggcgtt
     1981 tccacgaggt tggccaagtt gcggctatag cccgcgctcg atggcccgga ggagccgata
     2041 gcgcatcctg ccggtgttgc cccgcagcga gatcacgaag cgcacccgcc cgtgcttggc
     2101 ggtttcggca tagcccgcca gcgtgcggac gccgctcaat gtgccggtct tatatttgga
     2161 gccacggctg cccttgagca gatgggcatg gggcgcgaac aggtggagca cttgagcgag
     2221 gccgcgggcg gtgaagtggt tgccgcggct gatgccggag ccctcctcga gatggatgtc
     2281 ctcggccaag ccgttcgtgg caagaaatgc gttcgccacc ttaagggatt tctctaggct
     2341 cacggggccg cccagggtgc tgccaatctc caagaatact tggttggcga tgtaattgtt
     2401 cgagccgatg agaagttggc tgaggatctc ggagagcggg cgcgactgtt cgtgtacata
     2461 gaccggttcc aggccgtccg gcacggtccc gacggtgatc tcgcctttgc cgcttccgcc
     2521 ggcccgctcg ataaacgcgg cgagca
//
LOCUS       DBNHVU010000035         3819 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_533441, whole genome shotgun sequence.
ACCESSION   DBNHVU010000035 DBNHVU010000000
VERSION     DBNHVU010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_533441"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="AC16Y7_01335"
     CDS             complement(<1..299)
                     /locus_tag="AC16Y7_01335"
                     /inference="COORDINATES: protein motif:HMM:NF019460.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1636 family protein"
                     /protein_id="HIR5708052.1"
                     /translation="MTFTDITRDDDAENRSGTTVYVCVTCRRPGDPEDGPRPGLKLAR
                     ETALAADGTGVAVCEIECLANCSRSLSAALRCHGSWTYIFGGLEAGRDAKALIEGA"
     regulatory      complement(396..590)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     regulatory      668..868
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            906..1100
                     /locus_tag="AC16Y7_01340"
     CDS             906..1100
                     /locus_tag="AC16Y7_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtB domain-containing protein"
                     /protein_id="HIR5708053.1"
                     /translation="MTTHSTKPDTTGLDAAALTTSRAVPIFAAALLGIFIVGGVGFSH
                     MAVFHNAAHDYRHSMAFPCH"
     gene            1122..1865
                     /locus_tag="AC16Y7_01345"
     CDS             1122..1865
                     /locus_tag="AC16Y7_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbtA family protein"
                     /protein_id="HIR5708054.1"
                     /translation="MDYFRNIVVVAALAGALAGLGMTVAQYFVTVPLILKAETFEGQG
                     ATQATDASEATEATAAAHEHHAHEENAAAHDHGEGWAPEDGFERTFFNFLANLITGVG
                     FALLLVAASELFGGLHGWRQGVFWGLAGFTIFTLAPGLGLPPELPAMPAAELGARQAW
                     WAGTVVSAAIALGLLFYSRSLIATIAAIALLVLPHAIGAPQPISHDSPVPAGLHHSFV
                     VAVAMTTLVFWALLGGLAGYFRERFIRAA"
     gene            1867..2406
                     /gene="cobU"
                     /locus_tag="AC16Y7_01350"
     CDS             1867..2406
                     /gene="cobU"
                     /locus_tag="AC16Y7_01350"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HIR5708055.1"
                     /translation="MTQIEAAGDEGTTFVLGGARSGKSRFAESLVTARPRPWVYIATA
                     EPHDDEMAARIAEHKARREAGWETIEAPHELPEALDAAPRGATVLVDCLTLWLSNVMH
                     AQFDIEKVTQRLEEALHARVGTTVVVSNEVGLGLVPDNELGRAFRDAQGRLNQRIAFH
                     AGRVVFVVAGQPLIVKGSS"
     gene            2403..3020
                     /gene="cobO"
                     /locus_tag="AC16Y7_01355"
     CDS             2403..3020
                     /gene="cobO"
                     /locus_tag="AC16Y7_01355"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR5708056.1"
                     /translation="MTKAVDDKDAERHRAKMAKRKSVQDDEVAGKTLEKGLLIVHTGT
                     GKGKSTAAFGLALRMIGRGNRVGIVQFVKGAWHTAERDALEKFGDQVAWYSMGEGFTW
                     ETQDLARDVAAAERAWGKAVELMDDPSFSLVILDELNIALRYDYLPLEQVIATLKSRR
                     PDLHVVVTGRNAKPELIEAADLVTHMTLVKHHFAAGVKAQPGIEF"
     gene            3024..>3819
                     /locus_tag="AC16Y7_01360"
     CDS             3024..>3819
                     /locus_tag="AC16Y7_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020809838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HIR5708057.1"
                     /translation="MTARTLMFQGTGSDVGKSLIVAGLARALVARGLQVLPFKPQNMS
                     NNAAVTIDGGEIGRAQDLQARAARVPPSVHMNPVLLKPQSETGAQVIVQGRVYATAKA
                     ADFQSLKTELMKFVQDSFERLKADADIVLVEGAGSASEVNLRNNDIANMGFARACDVP
                     VVLIGDIDRGGVIASLVGTKAVIDVGDAAQMVGFLVNKFRGDPKLFETGMEKIASVTG
                     WPALGLIPFFAEARALPAEDALALDGAGTEKNDAVLKVAVPILPHIA"
BASE COUNT          671 a         1234 c         1225 g          689 t
ORIGIN      
        1 gccccctcga tcagggcctt cgcgtcgcga cccgcctcca ggccgccgaa aatgtaggtc
       61 cagctgccgt gacaacgtaa tgcggcgctg agcgaacggc tgcaattggc gagacattcg
      121 atttcgcaca cggcaacacc cgtcccgtcg gccgctagcg ccgtctcgcg ggcgagcttg
      181 agccctgggc gcggaccgtc ctcggggtcg cccggcctgc ggcaagtcac gcagacatag
      241 accgttgttc cagaacggtt ttctgcgtca tcgtcacggg taatgtcggt gaaagtcacc
      301 ccggaaagcc gctcctcggg cacatgcgtc tggggtgtgg gaccggagtt tagctccggc
      361 cttcgagcta accgccgccg aggcaccccg cccggccggc catcagatcg acggtaggtc
      421 tcctggcttt gcgggtcttt gccgtttgcc gccttcccgg gaatgacctc ccagtggctt
      481 taggctccgg ctcgccgctt acagttgcgg gggcagccgc ggatttggag gacttccgta
      541 ccgcgttccc tgttggctcg aaatgagcga ccgtcgatca ggcacttaag gggccggggc
      601 ccccctttgt caatccgctg tcaatgcgcc gcgcgggggc ccgcttgcgg cggcggcgaa
      661 ccccgctata gtcgctcccg tcgtcggttc ccgcatgagg cgggacgcga gaagggaacg
      721 cggtgaggga ttttcccgat tccgcggctg cccccgcaac tgttagcggt gagcgccctc
      781 gttaaggcca ctggagccag tctccgggaa ggccggggga aggcgttgac ccgcgagcca
      841 ggagacctgc cggcggctca acctggaacc gcgccctcgg gtggagggcg agagggggac
      901 aattgatgac gactcattcg accaagcccg acacgacggg cctcgacgcc gcagcgctga
      961 cgacatcgcg cgcggtcccg atctttgcag ccgctctgct cggcatcttc attgttggcg
     1021 gcgtgggatt ttcccacatg gcggtgttcc ataacgccgc ccacgactac cgccattcca
     1081 tggcctttcc ctgccactga cgtccaggga ggaccaggac catggattat ttccgcaaca
     1141 tcgttgtggt ggctgcgctc gccggcgcgc tggccggcct cggcatgaca gtcgcccagt
     1201 acttcgtcac cgtgccctta attctcaagg ctgagacctt cgaggggcaa ggcgcaactc
     1261 aggccaccga cgcaagtgag gccaccgaag caacagcggc ggctcacgag catcacgcgc
     1321 acgaagagaa tgccgccgcg cacgaccacg gagagggctg ggcgccagag gacggttttg
     1381 aacgcacctt cttcaacttc ctcgccaatc tgatcacggg cgttggcttc gctttgctgc
     1441 tggtcgccgc gagtgaatta ttcggcggcc tgcacggttg gcgccaaggg gtcttttggg
     1501 ggcttgccgg ctttacgatc ttcacactcg cgccaggatt aggcctgcca ccggaacttc
     1561 ccgccatgcc ggcggcggag cttggggcgc ggcaagcttg gtgggccggc acggtggtgt
     1621 ccgctgcgat tgcgctcggc cttctattct atagccggtc gctgatcgcc acgatcgcgg
     1681 cgatcgcgct cctcgtgctt ccgcacgcaa tcggcgcgcc gcaaccgatc agtcacgaca
     1741 gcccggttcc agcggggctg catcacagct tcgtcgtggc cgtggcgatg acgacgctcg
     1801 tgttctgggc gttgctcggc gggcttgcgg gctatttccg cgagcgcttc atccgcgccg
     1861 cgtgacatga cacagattga ggcagccggg gacgaaggga cgaccttcgt cctcggcggc
     1921 gcccgttccg gcaagagccg cttcgcagaa tctctcgtca ccgcgcggcc tcggccttgg
     1981 gtctacatcg cgacagccga gccgcatgac gatgagatgg ccgcgcgcat cgccgagcat
     2041 aaagcgcggc gcgaggcagg ctgggaaacc atcgaagcgc cgcacgaatt gcccgaagct
     2101 ctcgatgcgg cgccgcgtgg tgccaccgtc ctcgtcgatt gcttgacctt gtggctgtcc
     2161 aacgtgatgc acgcccagtt cgatatcgag aaggttacgc agcgtttgga ggaggcactg
     2221 cacgcccgcg tcggcaccac cgtggtcgtc tccaacgaag tcggcctcgg actcgtgccc
     2281 gacaatgaat tgggccgcgc ctttcgtgac gcgcaaggcc gcctcaatca acgcattgcg
     2341 tttcacgcgg gccgggtggt cttcgtggtc gcaggccagc cgctcatcgt gaaaggatcg
     2401 tcatgaccaa ggccgtcgac gacaaggacg ccgagcgtca tcgcgccaag atggcgaagc
     2461 gaaagtccgt tcaagacgac gaggttgccg gcaagactct ggagaagggc ctgctgatcg
     2521 tccacaccgg aacaggcaag ggcaagtcga ccgcggcctt cgggctggcg ctgcgcatga
     2581 tcggccgggg caaccgcgtc ggcatcgtgc agttcgtcaa gggcgcctgg catacggccg
     2641 agcgcgatgc gcttgagaag ttcggcgatc aagtcgcctg gtattcgatg ggcgaaggct
     2701 tcacctggga gacacaggac cttgcccgcg atgtcgcggc ggcggaacgc gcttggggca
     2761 aggctgtcga gctcatggac gatccgagct tcagcctggt cattctcgat gagctcaaca
     2821 ttgcgctgcg ctacgactac ctgccgctcg aacaagtcat cgccacgttg aagtcgcggc
     2881 gccccgatct tcatgtcgtg gtcacgggcc gcaacgccaa gccggaactc atcgaagccg
     2941 ccgacctcgt cacccatatg acgctggtca agcaccactt tgccgccggc gttaaagcgc
     3001 agcccggcat cgagttctga cccatgaccg cccgcacgct catgttccag ggcacgggct
     3061 ccgatgtcgg caaatcgctg atcgtcgcag ggctggcgcg cgcgcttgtc gcgcgggggc
     3121 tgcaagtgct gcctttcaag ccgcagaaca tgtcgaacaa cgcggccgtg accatagacg
     3181 gcggtgagat cggacgggcg caagacttgc aggcgcgcgc cgcacgcgta cccccaagcg
     3241 tgcacatgaa cccggttctg ttgaagccgc aaagcgaaac cggcgcgcaa gtcatcgtgc
     3301 aagggcgcgt ttacgccaca gccaaagcgg cggactttca gtcgttaaaa accgagctca
     3361 tgaaattcgt gcaagacagt ttcgaacgcc tcaaagctga cgccgacatt gtcctcgtcg
     3421 aaggcgccgg cagcgcatcc gaagtcaatc ttcgtaacaa cgacatcgcc aatatgggat
     3481 tcgcccgcgc gtgcgacgtg ccggtcgtgc tgatcggtga catcgaccgg ggcggcgtca
     3541 tcgctagtct cgttggcacg aaggccgtga tcgacgttgg cgacgccgcg cagatggtcg
     3601 gcttcctggt caacaagttt cgcggcgatc ccaaactttt tgaaaccggc atggaaaaga
     3661 tcgcgtccgt aaccggctgg ccggcgcttg gtctcattcc attctttgcc gaagctcgcg
     3721 cgctcccggc agaggacgcg ttggcgttgg acggtgccgg cacggaaaag aacgacgctg
     3781 tgcttaaagt tgcggtgccc atcctgccgc atatcgcca
//
LOCUS       DBNHVU010000036         4083 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1351782, whole genome shotgun sequence.
ACCESSION   DBNHVU010000036 DBNHVU010000000
VERSION     DBNHVU010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1351782"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1018)
                     /locus_tag="AC16Y7_01365"
     CDS             complement(155..1018)
                     /locus_tag="AC16Y7_01365"
                     /inference="COORDINATES: protein motif:HMM:NF022853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HIR5708058.1"
                     /translation="MNDFLYRAGMLLALSAFGAFLGVALGTPGLAASKGQSPWSKQTN
                     SKVRLVSGTLDADGTPALYAGVQLRMEPGWKTYWRNPGDSGVPPTFDWTGSKNVKSAE
                     VLYPMPHRFADAAGTAIGYDDEVVFPVKITPENAGEPVTLSLTFEYGLCKDLCIPSSV
                     DLRALLPADLGKGDALLIEQANAVVPEPAGHETLPRLEAVKATLDGPAPGLEIDALFA
                     DGATATDLFVASPELLVPVPKALGPLKAGKQRFAVAFASAEEAAAVKGKPLTFTLVSN
                     EGASDTVRTEE"
     gene            1212..1817
                     /locus_tag="AC16Y7_01370"
     CDS             1212..1817
                     /locus_tag="AC16Y7_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIR5708059.1"
                     /translation="MDSGSQTEDEGYLDGQLLIAMPAMGDPRFSRSVIYLCAHSSEGA
                     MGIIINQRAPNISFSELLEQLNIVPTEDRIALPAGLSAMDVHLGGPVETGRGFVLHSA
                     DYFKVESTLPIDETVCLTATVDILRDIAKGSGPNKALLALGYAGWAPGQLEDEIHANG
                     WLNCPANPELIFDPELDGKYSRALGSLGIDPIRLVNDSGHA"
     gene            complement(1808..>4083)
                     /locus_tag="AC16Y7_01375"
     CDS             complement(1808..>4083)
                     /locus_tag="AC16Y7_01375"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR5708060.1"
                     /translation="YRAAAEAGLFASLVLFLYTFLRIGLWHMWIKTVFWVWIAAQFAL
                     IAVAIIDPQTAAGLARASAFVIAGVGTALIAYFALRGQDRAMSLIPSWMLLIVWILGA
                     AVIVLGKLSGEVVISGLIAGLVLIIVLFGFTVTQFAFRTGGTAVSLGPPSLVQMKSLA
                     VDGSGTHVFQWHAGRDLITMGDEVEVELGLDPGALNGTVENFLQHMHASDRERFRLML
                     WSLQEKQGGDLQIDFRLRRHDGTYLWYDLRAHAAPSDNVRLLRCVGLMRDVTSLKRSH
                     ERLMHDAVHDSLTGLPNRELFMDRLQGAITRAAEGQSHRPTLIFIDIDRFKNVNKSFG
                     LVIGDSMLLTLGRRLGRHLNPQDTLARLGGDQFAILLISDSDPHHVATLAERVRRSLR
                     TPMKISAKEIVLTASIGIVVHDGSQATAQDMLRDGEIAVLRAKRGGADRIEIFNPGMR
                     TEDESRLPLESELRLALDRGQISVFYQPITRLASNQLAGFEALVRWDHPTRGRLVPED
                     FIPLAEESNLIVDLGSYVLNQALEEAASWHKVLPRDRDPLFVSVNVSSRQLFRQDMVQ
                     EIRLMLARESVPRGTLKLEITESLVMENPERAVEILAWLKTYGASLALDDFGTGYSSL
                     SYLHRFPFDVIKVDRSLVRDASQNGQTPLILRSVVALAHELGKEVVAEGVETQADAGY
                     LRSIGCEFGQGFYYGEPMAAKDVAALLAALASHRKRKQRERAHAPAPQTAPSGMEVDA
                     PSQGAIPALPGPSTAQA"
BASE COUNT          798 a         1283 c         1272 g          730 t
ORIGIN      
        1 gtcgacagac tctggatgcc ttcatcatcg aaaacccgga acgtgacgtc cggcaggcgg
       61 tctcctacgg caatgctcat cggggagtct cctcaatact gactttcgcg aggcacctta
      121 ccggctcacc tttgggacgc caaccgggcc tcggctattc ctctgtccgg accgtgtcgc
      181 tcgccccttc gttcgagacg agcgtgaagg tcagcggctt gcccttgact gcggcggcct
      241 cctctgcgct cgcgaaggca acggcgaacc gctgcttacc ggccttgagg ggcccgagcg
      301 ctttgggcac aggaacgaga agctccggac ttgcgacgaa gaggtcggtc gccgtggcgc
      361 cgtccgcgaa caacgcgtcg atctcaagac caggcgcagg accgtccagc gttgctttga
      421 ccgcttcgag gcgtggcagc gtttcatgcc ccgccggttc cgggacgacc gcgttggcct
      481 gctcaatcag cagggcgtct cccttgccga gatcggccgg aagcagggcg cgcagatcaa
      541 ccgaactcgg aatgcaaaga tccttgcaga gcccgtactc aaaagtcagc gacagcgtga
      601 cgggttcgcc cgcgttttcc ggcgtgatct tgacgggaaa gaccacctcg tcgtcgtaac
      661 cgatcgccgt gcctgcagcg tcggcgaaac ggtggggcat gggatagagc acctcggcgc
      721 ttttgacgtt cttcgaaccg gtccagtcga aggtcggcgg cacgccggaa tcaccagggt
      781 tgcgccaata ggttttccaa ccgggctcca ttcgaagctg aacgccggcg taaagtgcag
      841 gcgtaccgtc tgcatcaagc gtcccgctca ccagccgcac cttggaattg gtctgcttcg
      901 accaaggcga ttgccccttc gatgcggcaa gcccgggcgt gccgagggcg acgcctagaa
      961 acgcgccaaa tgccgacaag gcaagcagca tccccgcgcg gtacagaaaa tcgttcattc
     1021 ttgacataga ccttgcttca gaaattggct gaacacttga gcgaaaattt agcgctgcta
     1081 cggaaacggc gcgaccttgg aagggtcaag ttgcgtcacg cttgcgtgaa ccgtttgcgc
     1141 gaagaatgac agggccgcct taattcagcg ttgcctcttc cctggtggtg gaactgatgt
     1201 agcatggcct tatggacagc ggctcccaaa ctgaggacga aggctatctc gacggccagc
     1261 tcctcatcgc tatgccggcg atgggcgatc cgcgcttctc ccgctcggtg atctatctgt
     1321 gcgcgcactc ttcggaaggc gccatgggca tcatcatcaa tcagcgggcg cccaatatca
     1381 gcttcagcga actcctcgag cagctcaaca tcgtgccgac ggaagatcgc atcgcgcttc
     1441 ctgccggctt gtctgcgatg gatgtgcatc ttggcggacc ggtggagact ggccgcggct
     1501 tcgtgttgca cagcgcggac tacttcaagg tggagagcac gctgcctatc gatgagaccg
     1561 tctgccttac cgccactgtc gacatcttgc gtgacatcgc caaaggcagc gggccgaaca
     1621 aggcgctact cgcgcttggc tacgcgggct gggcgcccgg tcaattggag gacgagattc
     1681 acgcgaacgg ctggctcaac tgtcccgcca atccggagct gatcttcgat cccgaactgg
     1741 acggcaagta ttcgcgcgcg ctcggcagcc tcggcatcga tcccattcgg ttggtgaacg
     1801 actcaggtca cgcctgagcg gtgctcggcc ccggcagcgc cgggatggcg ccttggcttg
     1861 gcgcatcgac ttccatgccc gagggtgctg tctgcggcgc cggcgcgtga gcgcgttcgc
     1921 gttgcttccg tttgcgatgg ctcgccagcg cggcgagaag cgcggcgaca tccttcgccg
     1981 ccatcggctc gccgtagtaa aatccttggc cgaactcgca gcctattgaa cgcaaatacc
     2041 cggcgtcggc ttgtgtctcc acgccctcgg cgacgacttc tttgcctaac tcgtgcgcca
     2101 gcgccacgac cgaacgcaaa atcaacggcg tctgtccgtt ttgcgaagcg tcccggacca
     2161 acgagcgatc gaccttgatg acgtcaaagg ggaagcgatg cagatagctc aaggagctat
     2221 agccggtgcc gaaatcgtcg agcgctaggc tcgcgccata ggtcttcagc caggcgagaa
     2281 tttcgaccgc gcgctcgggg ttctccatca ccagcgactc ggtgatctca agcttcaacg
     2341 tgccccgcgg cactgactcg cgggccagca ttaaccggat ctcctgcacc atgtcctgac
     2401 ggaagagctg gcggctcgac acgttgacgc tgacgaacaa gggatcgcgg tcgcgcggca
     2461 gcactttgtg ccagctggcc gcctcctcca acgcctggtt caagacatag ctgccgagat
     2521 cgacgatgag attggattct tcggcgagcg ggatgaagtc ctcgggcaca agacgcccgc
     2581 gggtcgggtg gtcccagcgc accagcgcct cgaagccggc gagctggttc gacgcgagcc
     2641 gggtaatggg ctgatagaac acagaaatct gcccgcgatc caacgccaat ctgagctcgc
     2701 tctccagcgg cagccggctc tcgtcctcgg tgcgcattcc cggattgaag atttcgatac
     2761 ggtcggcgcc gccgcgcttg gcgcgcagca cggcaatctc gccgtcgcgc agcatgtcct
     2821 gcgccgtcgc ttgggagccg tcatgcacga cgatgccgat ggacgcggtc aggacaatct
     2881 ctttggcgct gatcttcatc ggcgtgcgca gcgaccgcct cacccgctcc gccagcgtgg
     2941 caacatgatg cggatcgctg tcagagatca acaggatcgc gaactgatcg ccaccaaggc
     3001 gcgccaacgt gtcttgcgga ttgagatgac ggccaagccg gcgccccaag gtcagcaaca
     3061 tggaatcgcc aatgacgaga ccgaagctct tattgacgtt cttgaagcga tcgatgtcga
     3121 tgaagatcaa cgtgggccgg tgagactgac cctcggcggc gcgcgtgatg gcgccttgca
     3181 ggcggtccat gaacagttcg cgattaggaa gccccgtcag gctgtcatga accgcgtcgt
     3241 gcatgagccg ctcgtgcgag cgcttcaggc tcgtcacatc gcgcatgaga ccgacacagc
     3301 gcaacagccg cacgttgtcg ctcggagcgg cgtgggcgcg aaggtcatac caaagatagg
     3361 ttccgtcgtg acgccgcaaa cggaagtcga tctgaagatc gcccccttgc ttctcctgca
     3421 gcgaccaaag cattagacgg aaacgctcgc ggtcggacgc gtgcatgtgc tggaggaagt
     3481 tttcgaccgt cccgttcagc gcaccggggt cgaggcccag ctccacctcc acctcgtcac
     3541 ccatggtgat gagatcgcgg ccggcgtgcc attggaaaac atgtgtgccc gagccgtcga
     3601 cggcgagcga cttcatctgc acgaggctgg ggggcccgag gctcaccgct gtgccgccgg
     3661 tgcggaaggc gaactgcgtg accgtgaagc caaagagcac gatgatcagc acgaggccgg
     3721 cgatgagacc cgagatgact acctctcccg acagtttgcc gagcacgatc acggccgcgc
     3781 cgagaatcca cacgatgagg agcatccaac ttgggatcag cgacatggcg cggtcctgcc
     3841 cgcgcaacgc gaaatatgcg atgagcgcgg tgccgacgcc ggcgatgacg aatgccgagg
     3901 cgcgggcgag gccggcggca gtttgcggat cgatgatggc gaccgcgatc aaggcgaact
     3961 gggcggcgat ccagacccag aacactgtct tgatccacat atgccacagg ccaatacgca
     4021 aaaaggtgta gaggaacagc acgaggctcg cgaaaaggcc ggcttccgcg gcggcccggt
     4081 aga
//
LOCUS       DBNHVU010000037         3907 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1574492, whole genome shotgun sequence.
ACCESSION   DBNHVU010000037 DBNHVU010000000
VERSION     DBNHVU010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3907)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3907)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3907
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1574492"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..1537
                     /locus_tag="AC16Y7_01380"
     CDS             269..1537
                     /locus_tag="AC16Y7_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR5708061.1"
                     /translation="MTGPRGTPRTAARVSRDAKAMRNETGESEGNALASAEASAHAGT
                     PAPGSAGAAEGNKGTGAFAQRPDHSVYGALDLGTNNCRLLVAKPSRRGFVVIDAFSRI
                     IRLGEGVLTSGKLSDDAMQRTIDALRVCGDKMRRRTVTRSRLIATEACRIATNGAEFV
                     DRVENETGLKLEVVGRETEAKLAVSGCASLLDWNCDWALVFDIGGGSSELIWLDLTRL
                     GKQRRRSVHDRVDVQNCIAAWTSLPIGVVSLAERHGGREVTEESYEAMVDDVMVGLNA
                     FESQYRFGEKIGRSSAHFLGTSGTVTTISGIHLKLPHYERSRVDGCWLTADDVRRVSG
                     DLIAMSYAERVAQPCIGHERADLVLAGCAILEALLRMWPCQRLRVADRGLREGILTTL
                     MAEDGHDRRGTSPRPRRRRRGGKKAQAGRD"
     gene            complement(1556..2974)
                     /locus_tag="AC16Y7_01385"
     CDS             complement(1556..2974)
                     /locus_tag="AC16Y7_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HIR5708062.1"
                     /translation="MPRYFVTLIVAVALFMETMDSTVIATSLPAIGADLGEDPIALKL
                     ALTSYLLSLAVFIPVSGWMADRFGAQTVFRVAIVVFTLGSAACGFAQGLADFVLFRIV
                     QGMGGAMMVPVGRLVILRSVPKSELISALAWLTVPALMGPVIGPPLGGFISTFFSWRW
                     IFWINIPIGLLGIYLATRFIENFREDEVPPLDVKGFFLSGVGLAGLAFGFTVIGQPFF
                     PPAFVVGLLTVGAIACTLYVRHALNTPNPLLDLRLLKIDTFFANIFGGFLFRIGVGAT
                     PFLLPLFLQLGFGMTALQSGLLTFATAVGAIAMKTTAAPIIRRFGYKRILVWNALLTS
                     VFVAACALFTAATPSAVILTVLLIGGFFRSLQFTAVNSIAYADIGEHDMSKATSFASV
                     AQQLSMSAGVAVGAFVLEIERMGRETNDVVASDFIPAFLVVAALAASSALIFMRLPKN
                     AGSSLSRKAHAVGEPEKQQSVE"
     gene            3063..3779
                     /locus_tag="AC16Y7_01390"
     CDS             3063..3779
                     /locus_tag="AC16Y7_01390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HIR5708063.1"
                     /translation="MVRPTKGGPAGRRGLNVRVKTAKGRKLSSTRWLQRQLNDPYVEE
                     AKRQGYRSRAAFKLIEIDDRYRLLKPGGVVIDLGAAPGGWCQVAAARVKAGEGKGQAQ
                     GKVIAADITDIEPVPGATFMQVDVTDAEAGEFVRESLGGARADVVLSDMAASAIGHRQ
                     TDHLRVIALAEAALDIAEDVLKPGGAYLAKVLQGGASGDLLTRLRKGFAKVAHVKPKA
                     SRQGSSEVYVLATGFRGAPA"
BASE COUNT          771 a         1129 c         1364 g          643 t
ORIGIN      
        1 ccgaggcccg tcctatcaag gagccggcgg gcgcggcgta gggcgctcaa cccaacgttt
       61 accctatagc cctgcgtcat cgacggaccc ccagtcgcgg cggtaacggg gcttgccacc
      121 gcttgagtgc ctgtctcatg aggccctgct tgcggaaacc ggctttacct gcctgagaac
      181 cttggtaaat tagcgacata ggatcaacgc cgcaggcggt cgggcaaggg aaagcgccgg
      241 cagctccggc ttgaaaggtt tgaccatcgt gactggaccg cgcggcaccc cccgcacggc
      301 tgccagggtt tcgcgtgacg ccaaagccat gcggaacgag accggcgaaa gcgagggaaa
      361 tgccttggcc agtgccgagg cgtcagccca cgcgggcacg ccagcgccag gctctgccgg
      421 ggcagccgag ggtaacaaag gtacgggggc gttcgcgcag cgcccggacc attcggtcta
      481 cggcgcgctc gatctcggca cgaacaattg ccgtcttctg gtcgccaagc cgtcgcgacg
      541 cggctttgtc gtgatcgatg ccttctcgcg catcatccgg ctgggcgaag gcgtgctgac
      601 ctccggcaag ctctccgacg atgccatgca gcgcacgatc gatgcgttgc gcgtttgcgg
      661 cgacaagatg cgccggcgca cggtcacgcg ctcgcggctg atcgcgacgg aagcttgccg
      721 catcgccacc aacggcgctg agttcgtcga tcgcgtggag aatgagaccg ggctcaagct
      781 cgaggtcgtt gggcgagaga cggaggccaa gcttgcggtt tccggctgcg cctcgctgct
      841 cgactggaat tgcgattggg cgctcgtgtt tgatatcggc ggtggcagct cggaactaat
      901 ctggctcgac ctgacacggc ttggaaagca acggcggcgt tcggtccacg atcgcgtcga
      961 tgtgcagaac tgcatcgccg cgtggacgtc gctgccgatc ggcgtggtca gcttggcgga
     1021 acggcatggt ggccgggagg tcaccgagga aagctacgag gccatggtcg atgacgtcat
     1081 ggtcgggctc aacgcgttcg agtcacagta tcgcttcggt gaaaagatcg ggcgctccag
     1141 cgcgcatttt ctcggaacgt cgggcacggt gacaacgatc tccggcatcc atctgaagtt
     1201 accccactac gagcgttcgc gggtcgatgg ttgttggctg acggcagacg atgtgcgccg
     1261 ggtgtccggc gacctcatcg ccatgagcta cgcggagcgg gtggcgcaac cttgcattgg
     1321 gcacgagcgc gcagatctcg tactggccgg ctgtgcgatt ctcgaagcgc ttcttcgcat
     1381 gtggccgtgc cagcgcctgc gtgtggccga tcggggtttg cgggaaggta ttctcaccac
     1441 actcatggcc gaggacggtc acgaccgccg cggaacctcg ccccggccgc gccggcggcg
     1501 ccgaggcggc aagaaagctc aggccggacg cgactaagcg cacaggctct tgcagctatt
     1561 cgacggattg ttgcttctct ggttcgccca ccgcatgcgc cttgcgggag aggctcgatc
     1621 cggcgttctt gggcaagcgc atgaagatca gcgcggaact tgctgcaagt gccgcgacca
     1681 cgaggaaggc gggaataaag tcgctggcca caacgtcgtt tgtctcgcgg cccatgcgtt
     1741 cgatctccag cacgaacgcg ccgaccgcga cgccggcgga catagaaagc tgctgagcga
     1801 cgctggcaaa gctcgtggcc ttgctcatgt cgtgttcccc gatatccgca taggcgatcg
     1861 agttgacggc cgtgaactgg agcgagcgga aaaagccgcc aatgagaagc acggtcagga
     1921 tgacggcaga tggggttgcc gcggtgaaga gcgcgcacgc ggcgacaaat acggatgtca
     1981 gcagtgcatt ccagaccaga atccgtttat agccaaagcg ccggatgatc ggcgcagcgg
     2041 ttgtcttcat ggcgatagcg ccaacggctg tggcaaaggt gagcaagccc gactgcaacg
     2101 ccgtcatgcc gaagccgagt tgcaagaaca gcggcagcaa gaacggtgtc gcgccgacgc
     2161 cgatgcggaa caagaagccg ccgaaaatat tcgcaaagaa cgtatcgatc ttgagcaagc
     2221 gcaagtcgag cagcgggttg ggcgtattga gcgcatggcg gacataaagc gtgcaggcaa
     2281 tagcgccgac cgtcagcagg ccgacgacga atgccggagg aaagaatggc tggccgatca
     2341 ccgtgaagcc gaaggcaagc ccggcgagcc cgacgcccga caagaagaag cccttgacgt
     2401 cgagcggcgg cacctcgtct tcgcggaagt tctcgatgaa gcgcgtggcg aggtagatgc
     2461 ccagcaagcc gatagggatg ttgatccaaa agatccaacg ccaggagaag aaggtcgaga
     2521 tgaagccgcc aagcggcggt ccgataaccg ggcccatgag cgcaggcacg gtgagccagg
     2581 cgagcgccga gatcagctcc gactttggca cgctgcgcag gatgacgagg cgtcccacag
     2641 gcaccatcat ggcgccgccc atgccttgca cgatgcggaa cagcacgaag tcggctaggc
     2701 cttgggcgaa gccgcaggcc gccgagccga gcgtgaaaac cacgatggcg acgcgaaaga
     2761 ccgtctgtgc gccgaacctg tcggccatcc agccgctgac gggaataaag acggccagcg
     2821 aaagcaggta tgaggtgagc gccagcttga gcgcgatagg atcctcgcca agatcggcgc
     2881 cgatcgccgg cagcgatgtt gcgatcacgg tggagtccat ggtttccatg aacagggcga
     2941 ccgcgacgat cagggtgacg aagtagcgtg gcatgtggca ggtgttagag aacggataga
     3001 gtggagatga ggctgggcgg tccgtccggt caaggcggtg cggcgatggg attgattagc
     3061 acatggtaag gccaacgaaa ggcggacctg cgggacggcg tgggctcaat gtccgggtca
     3121 agaccgcgaa ggggaggaag ctctcctcca cgcgctggtt gcagcggcag ctcaacgacc
     3181 cttatgtcga ggaggcgaag cgccagggct atcgcagccg cgcggccttc aagctcatcg
     3241 agattgatga caggtatcgc ttgttgaagc cgggcggcgt ggtgatcgat ctcggcgcgg
     3301 cgcctggcgg ctggtgtcag gtggccgcgg cgcgggtgaa agccggggag ggcaagggcc
     3361 aagcgcaggg caaggtcatc gccgccgaca tcaccgatat tgagcccgtt ccgggcgcca
     3421 ccttcatgca ggtggatgtg accgacgccg aagcgggtga gttcgttcgg gaatcgctgg
     3481 gcggcgcgcg cgccgacgtt gtgttgtccg atatggcggc atcggcgatc ggccatcgcc
     3541 agacggacca tttgcgggtg attgcgctgg ccgaagcggc gctcgatatc gcggaggacg
     3601 tgctgaagcc ggggggcgcc tatcttgcta aagtgctgca aggcggcgcg agtggcgatc
     3661 tcctcacgcg gctccgcaaa ggcttcgcca aggtggccca cgtcaagccg aaggcgagcc
     3721 ggcagggttc gtcggaagtc tatgtgcttg ccacgggatt ccggggtgcg cctgcatgaa
     3781 atggacacga catgattcgt tcacgggact gtgcttggcc gtgtgctgct ccagcgttgc
     3841 gagtcttgct agggtgagcg cgcatcgatt gggccggtcg gatagtggag ttgcacgatg
     3901 aatgtcg
//
LOCUS       DBNHVU010000038         6531 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_386432, whole genome shotgun sequence.
ACCESSION   DBNHVU010000038 DBNHVU010000000
VERSION     DBNHVU010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6531)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6531)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6531
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_386432"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="AC16Y7_01395"
     CDS             complement(<1..571)
                     /locus_tag="AC16Y7_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0140299 - molecular sensor activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5708064.1"
                     /translation="MAPSVGAPRQMLRRLREVMAEHVSAQARLDKVVMLIASNIVAEV
                     CSLYLRRRDGSLELVATEGLNIEAVHSTHLKPGEGLVGLVAEQAEPKQFADAQHHPAF
                     SYRPETGEEIYHSFVGVPILRGGHTVGVLTVQNRTMRQYSDEEVEALQTTAMVIAETL
                     ASGGIISDEVTEETRRDSSVRFTGKPISEG"
     gene            complement(659..1954)
                     /locus_tag="AC16Y7_01400"
     CDS             complement(659..1954)
                     /locus_tag="AC16Y7_01400"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR5708065.1"
                     /translation="MSLLVLKFGGTSVADLERIRNAATHIKREADAGNACAVVVSAMA
                     GQTNQLVSLVEEAWGYEERSQNGGEASAHYDMREYDAVVSSGEQVTSGLLALVLQAMG
                     VKARSWQGWQIPLHTDTAHRAARITGIDVGALKASIQKGEVAVVAGFQGIAPGNRIAT
                     LGRGGSDTSAVALAAALQADRCDIYTDVDGVYSTDPRVVPKAHRMDRISYEEMLEMAS
                     QGAKVLQTRSVELAMQKGVRVLVRSSFDAPDIVAKGDAETGEGIGTLVCHEDEIVEQN
                     VVSGIAYARDEAKVTLLKVADKPGVAARVFGPLADAHINVDMIVQNVSEDGRSTDLTF
                     TVQSADLDRALEVLKKAKDTIGYLDLRGSTDIAKVSAIGVGMRSHAGVAAQMFSALAE
                     KGINIEAISTSEIKISVLIDAAYAELAVRTLHSLFGLDS"
     gene            2089..2841
                     /gene="ubiG"
                     /locus_tag="AC16Y7_01405"
     CDS             2089..2841
                     /gene="ubiG"
                     /locus_tag="AC16Y7_01405"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HIR5708066.1"
                     /translation="MTAAPDNAAMLDGDEVARFAKLAGEWWDETGPFRPLHRINPTRL
                     TFIRDRLCAHFQRDPKAVPSLTGLSVLDIGCGGGLVAEPLTRLGATVTGIDPAAETIE
                     AAKTHARGARLSITYEATTAEALADAGQSFDAVLLLEVVEHVPDVPAFLKRIAPLVAP
                     GGMMILSTLNRTLKAYALAIVCAEYVLRWLPAGTHQWDRFVRPDELQTALCGAGLELA
                     NTTGMVYDPLADEWSLSTDTDVNYFAAATRAA"
     gene            complement(2852..3688)
                     /locus_tag="AC16Y7_01410"
     CDS             complement(2852..3688)
                     /locus_tag="AC16Y7_01410"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR5708067.1"
                     /translation="MRLSNLFRLRNLSFELIAGGLFAVLLASPLHAEPTHALDLDVDA
                     DTLAGPQDDSVTAQPEPVYPNGEPGIETVEGDDANEVVNAKPQVVASIDKGTQSMTVF
                     VDGVEQHTWKVSTGIGGYSTPSGNFTASSMNKIWYSRQWDNAPMPHAVFFTKKGHAIH
                     ATNETKKLGKPASHGCVRLSPKNAETFFNLVKEAGLKNTQVELVGVTPGGEYKVSQPP
                     RDRYPEYGRRYRDRYGSYEERQPPVYRKRRRLFRPYYDSPPRQQRQPRQRRRWFRRNN
                     GF"
     gene            complement(3834..4280)
                     /locus_tag="AC16Y7_01415"
     CDS             complement(3834..4280)
                     /locus_tag="AC16Y7_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HIR5708068.1"
                     /translation="MIRFALSCDEGHSFEAWFSSGASYDEQIEAEVIRCPACGSAAVR
                     KAPMAPAVVRGRGKNQDQTAAEAPELPANPEQKQSFAILRGLREHLKANADDVGEKFP
                     DEARKIHYGEAEARDIYGEATSDEATSLNKEGIPVLPLPKLPEEHD"
     gene            complement(4277..5155)
                     /locus_tag="AC16Y7_01420"
     CDS             complement(4277..5155)
                     /locus_tag="AC16Y7_01420"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR5708069.1"
                     /translation="MSIPSDRRFRAALVQLRAGRTIAPNLEAAEALIREAARGGAQYV
                     QTPENTALMELEPALVLAQASPEASSEPLQVFCALAAELGIWLHVGSLGIQADDTRVA
                     NRSFLIDPKGAIAARYDKLHMFDVDLAGGESYRESQYYAPGSKAVVADLPWGRLGMTV
                     CYDLRFPALYRALATAGANLIAIPAAFTKQTGQAHWHVLLRARAIETGSFVLAATQGG
                     LHENGRETYGHSMIVSPWGDILAEAGGDPCVIFAEIDLARSDEARAAIPALKHGRPFE
                     IEPAGDEPDFVRKELS"
     gene            complement(5252..5611)
                     /locus_tag="AC16Y7_01425"
     CDS             complement(5252..5611)
                     /locus_tag="AC16Y7_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012383.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR5708070.1"
                     /translation="MPVPPYHSRLWWRRFNQSALLSQELSKHTSTPVDCFALKRVRQT
                     ASQVGFTAEQRKKNMAGAFKVDGARELTLSGKRIVLVDDVITTGATAEACARVLRRAK
                     AARVDVVVLARAVEPAL"
     gene            complement(5614..5799)
                     /locus_tag="AC16Y7_01430"
     CDS             complement(5614..5799)
                     /locus_tag="AC16Y7_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708071.1"
                     /translation="MLSAAAIASPPAYDRARAVARYSGTMRDLIQSFKYRDRQEGLGL
                     FGWWLARGGPNSWKTPI"
     gene            complement(5712..6185)
                     /locus_tag="AC16Y7_01435"
     CDS             complement(5712..6185)
                     /locus_tag="AC16Y7_01435"
                     /inference="COORDINATES: protein motif:HMM:NF039185.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HIR5708072.1"
                     /translation="MGNQGLALGDLMGEAGPAPAEQSAVEEGLVIHCHRLACFPCSPS
                     RCIIGPMSMSEAQFRRSHSWRAAFGVGLDLLLRPVCIACRARVSRHGLVCGACFAGID
                     FIAPPICDRLGVPLPFDTVGLPIPAGLCCRRRQSPRRRPTTARGPWRATLGPCAI"
     gene            6090..>6531
                     /locus_tag="AC16Y7_01440"
     CDS             6090..>6531
                     /locus_tag="AC16Y7_01440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5708073.1"
                     /translation="MNDQALFDRTLLRRRRARFAHEITEREALIAHVAREIAERVSLM
                     LREFPLVLDLGAYHGLLGQAIAALPSVGEVVYAESTFAYAEQCPRPALVCDEDLLPFK
                     DASLNLIVSGLALHRVNDLPGSLIQIRRALAPDGLFMAAALGARA"
BASE COUNT         1152 a         2166 c         2013 g         1200 t
ORIGIN      
        1 cgccctcgga gatgggcttg ccggtaaaac gcacacttga gtcgcggcgc gtctcttcgg
       61 tgacctcgtc ggagatgatg ccgcccgagg caagtgtctc ggcaatcacc atcgccgtgg
      121 tctgaagcgc ctcgacctct tcatcgctgt attggcgcat ggtgcggttc tgcaccgtga
      181 gcacgccgac cgtgtgcccg ccgcgcagga tcggcacacc gacaaaggag tgatagatct
      241 cctcgcccgt ctccggccgg taggagaagg ccggatgatg ctgagcatcg gcaaactgct
      301 tcggctcggc ctgttcggcg acgaggccga ccagtccctc gccgggcttg aggtgcgtgc
      361 tgtggaccgc ttcgatgttc aggccctcgg tggccacgag ttcgagcgag ccgtcgcgcc
      421 gccgcaaata gagcgagcac acctccgcca cgatgttgga cgcgatcagc atcacgacct
      481 tgtcgaggcg agcctgggcg ctcacgtgct cggccatcac ctcacggagg cgccggagca
      541 tctgtcttgg agcaccaact gacggagcca tagtcttcag tcctcgctgc ccgccttttc
      601 ccggcggacc cgagccttcc cgatcttcac agaccagatt agcctttttt ggcgtctact
      661 agctgtctag cccgaataga gaatgcaagg ttcgcaccgc aagttcggca taagccgcat
      721 cgatcaatac gctgattttg atctccgaag tggaaatcgc ttcaatattg ataccttttt
      781 cggcaagtgc gctgaacatc tgggcggcga cgccggcatg gcttcgcata ccgacaccaa
      841 tggccgacac cttggcgatg tccgtcgatc cgcgcagatc cagatagccg atcgtgtcct
      901 tggccttctt caggacctcc agagcgcgat cgagatcggc ggactgcacc gtgaacgtga
      961 gatcggtcga gcggccgtcc tcggagacgt tctgcacgat catgtcgaca ttgatatgtg
     1021 cgtcggccaa aggaccgaac acccgggcgg cgacgcccgg cttgtcggcc accttgagca
     1081 gcgtcacctt ggcttcgtcc cgcgcataag caattccact gacgacgttc tgctccacga
     1141 tctcatcctc atggcaaaca agtgttccaa tcccctcgcc cgtctcggca tcgcccttgg
     1201 cgacgatatc gggcgcatcg aagctcgaac gcaccagcac gcgcacgccc ttttgcatgg
     1261 cgagctcgac ggagcgcgtc tgcagcacct tggccccttg cgaggccatt tctagcatct
     1321 cctcatatga aatgcggtcc atgcggtgcg ccttcggcac cacgcgggga tcggtcgaat
     1381 agaccccgtc cacgtcggtg tagatgtcgc agcgatccgc ttgaagcgcc gcggcgagcg
     1441 cgaccgcgct cgtgtccgaa ccgccccggc cgagcgtggc gatgcggttg ccaggcgcaa
     1501 tcccttggaa gcccgcgacc acggcgacct cacccttctg gatagaggct ttgagcgcgc
     1561 cgacatcgat gccggtgatg cgcgcggcac gatgggcggt gtccgtgtgg agcggaatct
     1621 gccagccctg ccaggaccgg gccttgacgc ccatggcctg gagcaccagc gccaaaaggc
     1681 ccgacgtcac ctgctcgccg gaggacacca cagcgtcgta ctcacgcatg tcataatgag
     1741 ccgaggcctc gccgccattc tggctgcgct cctcatagcc ccaggcctcc tcgacgagag
     1801 agacaagctg gttagtctgc ccggccatgg ccgaaaccac caccgcgcaa gcgtttccgg
     1861 cgtccgcctc gcgcttgatg tgcgtggcgg cgttgcggat acgctccaga tcggctaccg
     1921 acgtgccgcc gaatttcagg accagcaagg acatgactgc tcacaacaag agggccggcg
     1981 ccctttttgg cccggccagg aaaatccggc tctttcatac tgtttagggc gcaacgccgc
     2041 aacatggctg tcttgacagg ctggcgcttg ggccggccaa tgcgagccat gacagccgcc
     2101 cccgacaatg ccgccatgct cgacggcgac gaggttgccc gctttgccaa gctcgctggc
     2161 gaatggtggg atgagaccgg cccctttcgc ccgctgcatc ggatcaatcc cacccgcctc
     2221 acctttatcc gcgaccggct ctgcgcgcat tttcagcgcg atccgaaggc ggttccgagc
     2281 ctcaccggcc tttcggtgct cgatatcggc tgcggcggcg gcctggtcgc cgagcctttg
     2341 acccggctgg gcgcgaccgt cacaggcatt gacccggcgg ccgagaccat cgaggccgcc
     2401 aagacccacg cgcggggcgc gaggctctcg atcacctacg aggccacaac cgcggaagcc
     2461 ctggcggatg cgggacagag cttcgatgcg gttcttctgc tggaggtggt ggagcatgtg
     2521 cccgacgtgc cggcgttcct gaagcggatc gccccgctcg tcgcgccagg cggaatgatg
     2581 atcctctcca cgctgaaccg tacgctcaaa gcctatgcgc tcgccattgt ctgtgcggag
     2641 tatgtccttc ggtggctgcc cgccggcacc catcaatggg atcgcttcgt gcggcccgac
     2701 gagttgcaga cggcgctgtg tggtgcaggt ctcgaactcg ccaacaccac gggcatggtc
     2761 tacgacccgc tcgcggatga atggagcctg tcgaccgaca cggacgtgaa ctatttcgcc
     2821 gcggcgaccc gcgccgcttg agccttctcg cctagaagcc gttgttgcgg cggaaccagc
     2881 ggcgccgttg ccgcggttgc cgttgctggc gcggtggcga gtcgtaataa ggcctgaaca
     2941 gacggcggcg cttccggtag acgggcggct gccgctcctc gtaagagcca tagcgatcac
     3001 gatagcggcg cccgtattcg ggatagcggt cgcgcggcgg ctgcgagacc ttgtattcgc
     3061 cgcctggcgt cacgccgacc aattccactt gggtgttctt gaggcccgct tccttgacca
     3121 agttgaagaa cgtctcggcg ttcttgggcg atagacgcac gcaaccatgc gaagccggct
     3181 tgccgagttt cttcgtttca ttggtggcgt ggatcgcgtg tcccttcttg gtgaagaaca
     3241 ccgcatgcgg catcggcgca ttgtcccact gccggctata ccaaatcttg ttcatcgagc
     3301 tggcggtaaa gttgcccgac ggcgtggaat agccgccaat gcccgtcgag accttccagg
     3361 tatgctgctc gacgccatcg acgaagacgg tcatcgactg cgtgcccttg tcgatgctgg
     3421 ctacgacctg gggcttcgcg ttgacgactt cgttcgcgtc gtcgccttcg acggtctcga
     3481 tcccgggctc gccgttcgga tagaccggct ccggctgcgc ggtgacactg tcatcctgtg
     3541 ggccggcaag cgtgtcagca tcgacgtcga gatctagagc gtgcgtcggc tctgcatgaa
     3601 gcgggcttgc gagcagaact gcgaacagcc ctccggcgat cagctcaaaa gacagatttc
     3661 gaagtcggaa aagatttgag aggcgcatcg ttcgttccct gctttacacg caaaccaccg
     3721 cccgacagcc tcaatatagg gggccaacaa ggctttacca gggcgcctgg cgagaagtga
     3781 ttctccttat aggccaaggg cccaaacaac aggttacccc gaatgcccgt cggtcagtcg
     3841 tgctcctccg gcagcttggg caacggcaga acgggaattc cctctttgtt caatgacgta
     3901 gcctcatccg acgtcgcttc accgtagatg tcccgtgcct ccgcttcccc gtagtggatc
     3961 ttgcgggcct catcggggaa cttttccccg acatcgtctg cattggcctt cagatgctcg
     4021 cgcaggcctc tcaggatggc aaagctctgc ttctgctccg gatttgcagg caattcgggc
     4081 gcctcggcgg ccgtctggtc ttggttcttt ccgcggcccc gcacgaccgc cggcgccatg
     4141 ggcgccttcc gcaccgccgc acttccgcag gccgggcagc ggatgacctc ggcctcgatc
     4201 tgctcgtcat aggacgcgcc ggacgagaac caagcctcga agctatggcc ttcatcgcaa
     4261 gagagcgcaa agcggatcac gatagttcct ttcgtacaaa gtcgggctcg tccccagccg
     4321 gctcgatctc gaacgggcgc ccatgtttga gtgcggggat ggccgcccgc gcctcgtcgg
     4381 accgtgccaa gtcgatctcg gcgaagatca cgcaagggtc cccgcccgcc tcggccagga
     4441 tgtcgcccca gggcgacacg atcatgctgt gtccgtatgt ttcccggcca ttctcgtgca
     4501 gaccgccttg ggtggcggcc aacacgaacg atcccgtctc gatcgcccgt gcccgtaaca
     4561 gcacatgcca atgtgcctga ccggtctgct tggtgaaggc agcaggaatg gcgatgaggt
     4621 tggcgccggc cgtggcgagc gcacgataga gcgcggggaa ccgcaggtca tagcaaacgg
     4681 tcatgccgag tcgcccccat ggcagatcgg caacgaccgc cttggaaccg ggcgcatagt
     4741 attgcgactc acgatagctc tcgccacctg ctaggtcgac atcgaacata tgcagcttgt
     4801 cgtagcgcgc ggcgatcgcg cctttggggt cgatgagaaa ggagcggttg gcgacgcggg
     4861 tgtcgtcggc ctggataccg agcgagccca catgcagcca aatcccgagt tcggccgcaa
     4921 gcgcgcagaa gacctgcaag ggctcactcg atgcttcggg actcgcctgc gccagcacaa
     4981 gcgccggctc aagctccatg agcgcggtgt tctccggcgt ctgcacatat tgggcgcccc
     5041 cgcgcgcggc ctcgcggatc aatgcttcgg ccgcttccag attgggcgcg atggtccggc
     5101 ccgcccgcaa ctgcacaagg gcggcgcgga accgccggtc tgagggaatg ctcattggta
     5161 tctcatcgtc cgaggctgaa gcgtctcagg tcatcgtaac tggcccgata accggcctgg
     5221 cgcaaggcca aaccctacgc ccacacctag atcacaaagc cggctcgacg gcccgggcaa
     5281 gcaccacgac gtccacgcgc gcggccttgg cccgtctcaa cacccgcgcg caagcctccg
     5341 ccgtggcgcc cgtggtgatg acatcgtcca ccaagacgat gcgcttgcca ctgagtgtga
     5401 gctcacgcgc accatcgact ttgaaggcac ccgccatgtt cttcttgcgc tgctcggccg
     5461 tgaacccaac ttggcttgcc gtctgccgca cgcgtttcaa ggcgaagcag tcgacgggag
     5521 tgctcgtgtg tttcgacagc tcttgggaaa gaagcgctga ctgattgaac cggcgccacc
     5581 acagccgcga gtggtagggc ggcacgggca cgatcaaatc ggcgtcttcc aggagttggg
     5641 cccgccgcgc gcaagccacc agccgaacag gccaagcccc tcctggcggt cgcgatattt
     5701 gaagctttgg atcagatcgc gcatggtccc agagtagcgc gccacggccc gcgcgcggtc
     5761 gtaggccggc ggcgaggcga ttgccgccgc cgacagcaaa ggcccgccgg tatcggaagg
     5821 cccactgtat cgaaaggcag cggcacaccg aggcggtcgc agatcggagg cgctatgaaa
     5881 tcgatccccg cgaaacacgc cccgcagact agcccgtgcc tgctgacgcg cgcacggcag
     5941 gcgatgcaga ccggccgcag caagaggtcc aggccgacac cgaacgccgc gcgccaggaa
     6001 tggctccgcc ggaactgggc ttcgctcatc gacatcggac ctatgataca gcgtgacggt
     6061 gagcacggga aacacgcaag cctatgacaa tgaatgacca ggccctcttc gaccgcactt
     6121 tgctccgccg gcgccgggcc cgcttcgccc atgagatcac cgagcgcgag gccctgattg
     6181 cccatgtggc gcgggagatc gccgagcgcg tgagtctgat gctgcgggag tttcccttag
     6241 tgctcgatct cggcgcctat cacggcttgc tcggccaagc gatcgccgcg ctgccctccg
     6301 tcggcgaggt tgtctatgcg gagagcacgt tcgcttatgc agagcagtgt ccgcgtccag
     6361 cgctggtgtg cgacgaggac ctcctgccgt tcaaggacgc gagcctcaac ctgatcgttt
     6421 cggggctggc ccttcaccgc gtcaacgatc ttcccggcag tctgatccag atccgacgcg
     6481 cgctggcgcc agacggtctg ttcatggctg cggccttagg cgcgcgcgcg c
//
LOCUS       DBNHVU010000039         4923 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1649861, whole genome shotgun sequence.
ACCESSION   DBNHVU010000039 DBNHVU010000000
VERSION     DBNHVU010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4923)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4923)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4923
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1649861"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="AC16Y7_01445"
     CDS             <1..416
                     /locus_tag="AC16Y7_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HIR5708074.1"
                     /translation="AFAEALHEKVRRELWGYAPDETFSNEQLIAENYAGIRPAPGYPA
                     QPDHTEKRTLFTLLDAENKIGVKLTESCAMWPAAAVSGLYFSHPESRYFGIGKIGRDQ
                     VADYAARKGWTVEEAERWLGPILSYDPKRAADAAA"
     gene            complement(849..1253)
                     /locus_tag="AC16Y7_01450"
     CDS             complement(849..1253)
                     /locus_tag="AC16Y7_01450"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5708075.1"
                     /translation="MPPWSQMGYSAEVMTRFLAARDDGARRYVIDVDGAPAGAVSIRY
                     PWLLGPYVELLAILPASQGRGLGAAVLDWLAQEASRLEARNLWVCASRGNASALRFYQ
                     RHGFEETAALPDLVADGFEDILLRRTLFHSFS"
     gene            complement(1324..2376)
                     /locus_tag="AC16Y7_01455"
     CDS             complement(1324..2376)
                     /locus_tag="AC16Y7_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR5708076.1"
                     /translation="MALIRAVLSGLRALAVFCVSTAHAEDIGSTNNLGPAEDAGVLAR
                     CWTSEALAGSKAELKAPPSATRFDLKALRTEALPDPEPVSEELRGSIRSVDLPDGEKL
                     IALTFDLCESGGRRAGYDYRVVDLLRKEGIKATFFAGGKWLESHPERAQQLIADPNFE
                     IGGHGLRHRDFTKASKATLSDEIRLTEAAYARAKSALKTRACATDPAPDIQTRMTLMR
                     FPYGRCNAKALTAVAEAGQLAVQWDIVTGDPDPRISAKTMANTILTKAHPGAIVVAHA
                     NGRGRRTAEALALALPKLKSQGYEFVTVGELLAAGKPVIAETCYLSRPGDNRARVARA
                     KRRKTKNGSLSTFRQR"
     gene            complement(2436..3182)
                     /locus_tag="AC16Y7_01460"
     CDS             complement(2436..3182)
                     /locus_tag="AC16Y7_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR5708077.1"
                     /translation="MQRRGIGFADHLPSRLIAGIAAIAVAGVASLAVGQSAKKGPCTD
                     DAMIVFDASGSMSGNQTLGIPNSRPRIDELRSALGHVLPSATRFRRVGLVTYGPGPYN
                     QCNVTLNLKPTIDAAKPIMRVVNRLIPAGKTPLTEGFEQAAEALDFRNKPGVIVLVTD
                     GEETCGRSPCELAKLLHQEAYQLTIHVIAFRYSGYSWTGGNNFMDLMCVADENNGLYI
                     KANDEGELIDALEKTLDCPMVSQALLRQIR"
     gene            complement(3283..4458)
                     /locus_tag="AC16Y7_01465"
     CDS             complement(3283..4458)
                     /locus_tag="AC16Y7_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_function: GO:0015369 - calcium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA];
                     GO_process: GO:0070588 - calcium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium:cation antiporter"
                     /protein_id="HIR5708078.1"
                     /translation="MKERTAGSRRKRVPKKTAAKTPPPFRLTSIAPAIAGILTTIVFF
                     AFGDTFLADLSNHLRTVVLFAFLFGVMMWCAFAVVTHADAVAEYLGEPYGTLILTLSV
                     IVIEVALLASIMIHGENNPTLARDAMFATLMIVLNGMVGIALLIGALRYWEQDYNLAG
                     ARSFLVVITALSVFALILPNYTKTPPDPINAPGKAWMFLAVTLLFYGVFLAIQTLRHR
                     AFFDEPAEAVAASPKRGDRKAATGSLAYHVAMLILSLLPVVLLAEYLAVIVDYGIEDL
                     DLPDELGGILIAILVLAPEALTAFHAALANRLQRAVNVCLGSALSTIGLTIPAVLLIG
                     MVTGRDVHLGLTGTETVLLVLTLFISGLTFGGGRTNVLQGVVHLLLFFVYILLIFSP"
     gene            4617..>4923
                     /locus_tag="AC16Y7_01470"
     CDS             4617..>4923
                     /locus_tag="AC16Y7_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5708079.1"
                     /translation="MTHSGRGWRRSGSYHHGNLKEVLLEAARKLIEEHGAFGFSLTEA
                     ARLAGVSPAAPYRHFRDRDALLAEVARSGFERFAARLDAAWNNGIPTPLSAFENLGRA
                     "
BASE COUNT          939 a         1485 c         1606 g          893 t
ORIGIN      
        1 aagcttttgc cgaggcgcta catgagaagg tgcgccgcga actgtggggt tacgcgccgg
       61 acgagacgtt ctctaacgag cagctcatcg ccgagaatta tgccggcatc cgtccggcgc
      121 ccggctatcc ggcccagccc gaccacaccg agaagcgcac gctgttcacg ctgctcgatg
      181 cggagaacaa gatcggcgtg aagctcacgg agtcttgcgc catgtggccc gcggccgccg
      241 tgtcgggtct ctatttctcc caccccgaga gccgttattt cggcatcggc aagatcggcc
      301 gcgaccaagt cgccgattac gccgcacgaa agggctggac cgtcgaggag gccgagcgtt
      361 ggctcgggcc catcctcagc tacgatccca agcgggccgc agacgccgcc gcgtaagaca
      421 acgccgacgt ccgagaccgc ccaggcgatt aggttcgccg gaggcgccac acttcattga
      481 actgtcggtg gcggccctaa ggggccgaaa gcctcgctct tagatcatcc ctaggatgac
      541 ggcggcgagg aaaccgaaag atacgatcgc catcacggcg gtcagtttgg ctgtgagttc
      601 catcggaagc tctcctttcc gattggaggg tgcccgttac cgaactgtcg gattttactg
      661 ggtcccctca agtcgtgcca aggtgttttc ttgctgcgcc gcacaattgt gaagagctgc
      721 aagctgatgc aaacaacgtt gattgggcct ttttggttcg tcccgaaccg aaattttttt
      781 ccaaatcccg gatttccgcc acgaatgggg cgggtccgtt tgagcgcgga acgtggcgcc
      841 taaggcggtc aggaaaagct gtggaaaagc gtacggcgca gcaggatatc ctcgaacccg
      901 tcggcgacga ggtccggcag cgcggcggtc tcctcaaatc cgtgacgctg gtagaagcgt
      961 aaggccgacg cgttgccgcg cgaggcacac acccagagat tccgggcctc taagcggctt
     1021 gcctcctgcg cgagccaatc gaggacggcc gcccccaagc cgcgcccttg ggatgcgggc
     1081 aggatggcca gcaactcgac atacggccca aggagccagg ggtagcggat cgagacggcg
     1141 ccggcgggcg cgccgtcgac gtcgatgaca tagcggcggg cgccatcgtc gcgagcggcg
     1201 agaaagcgcg tcatcacctc ggcggagtag cccatctgcg accacggcgg caacgcgaca
     1261 atggcctcag cgagcctggc gcagtcttgt tccgaaggtg ggcgaagccg tccaaccgaa
     1321 agatcagcgc tgccggaaag tggagagtga tccattcttt gtcttgcgcc gtttggcgcg
     1381 cgccacgcga gctcgattgt ctcctggacg actgagatag cacgtttccg cgatgacggg
     1441 ttttcctgcg gcgagcaatt cgccgaccgt gacgaactcg tagccttggc tcttgagctt
     1501 cggcagggcg agcgcgagag cttctgcggt tctccgaccg cgtccgttcg cgtgcgccac
     1561 gacgatggcg cccggatggg ccttcgtgag aatcgtattg gccatggtct tggccgagat
     1621 gcgcggatcg ggatcgcccg tcacgatgtc ccattggacc gcgagctgcc ccgcctcggc
     1681 caccgcggtc agcgccttgg cattgcaccg cccgtaagga aaccgcatca aggtcatgcg
     1741 tgtctgaatg tcgggcgcgg ggtccgtggc gcaggcgcgg gtcttaagag cgcttttcgc
     1801 ccgggcatag gcggcctcgg tcagacggat ctcgtcgctc agcgttgcct tgctcgcctt
     1861 cgtaaagtct cgatgacgaa gaccatggcc gccaatttcg aaattggggt cggcaatcaa
     1921 ttgctgggcg cgctcaggat ggctctccag ccactttccg ccggcgaaga aggttgcttt
     1981 gatgccctcc ttccgcagga gatcgaccac gcggtaatca tatccggcgc ggcggccgcc
     2041 gctttcgcac aagtcgaagg taagcgcgat cagcttctcg ccgtccggca gatcgacgct
     2101 gcggatcgag ccgcgcaatt cctccgatac cggctccgga tcgggcagag cttccgtcct
     2161 cagcgctttc agatcgaacc gcgtggcgga cggcggagcc ttgagctcag ctttcgaccc
     2221 ggcgagcgcc tcggaggtcc aacagcgcgc gagcacgccg gcgtcttcgg ctgggccgag
     2281 gttgtttgtt gagcctatgt cttcggcatg tgccgtgctc acgcagaaaa ccgcgagcgc
     2341 gcgcaacccc gacaacaccg cacggatcag cgccataagg catcgtcccc tttgggcaat
     2401 ctatcggacc gtcgcgtgct gagtgcgacg aaagactagc gaatttgcct gagcagagcc
     2461 tgggagacca tcgggcaatc caatgtcttt tccagagcgt cgatgagctc gccctcgtcg
     2521 ttggccttga tgtacaggcc gttgttttcg tcggcgacgc acatcagatc catgaagttg
     2581 ttgccgccgg tccaggagta tccggaatag cggaacgcga tgacgtggat ggtgagctgg
     2641 tacgcttctt ggtggagcag cttggccagc tcgcaaggtg agcgcccgca ggtttcctcg
     2701 ccgtcggtca ccaggacaat gacgcccggc ttattgcgga agtcgagcgc ttcggccgct
     2761 tgctcaaagc cctcggtcag cggcgtcttg ccggccggga tcaaccggtt caccacgcgc
     2821 atgatgggct tggcggcgtc gatggttggc ttgaggttga gggtgacgtt gcactgattg
     2881 tagggacctg gaccataggt cacgaggccg acgcggcgga accgcgtggc actcggtagg
     2941 acgtggccca gtgctgaacg caactcatct atgcgcgggc gtgaattcgg gatacccaga
     3001 gtttggtttc cggacatgga gccggacgca tcgaagacga tcatggcgtc gtcggtgcaa
     3061 gggccttttt tggcgctttg cccgacggcc aggctggcca cgccagcgac agcgatcgcg
     3121 gcgatgccgg cgatcagccg agagggcaga tggtcggcaa atccaatgcc acgacgctgc
     3181 atatgacgac actcccttgg taatttcggc gcgagtgtag cgaaattccg catatgaagg
     3241 gaatagcagg aattccggga cgtccgctgc ccgtccgtcc gctcaagggc tgaagatcaa
     3301 aaggatatag acgaagaaca gaagcaggtg gacgacgcct tgcagcacgt tggtgcgccc
     3361 gccgccgaat gtgaggccgc tgatgaaaag ggtgaggacc agcagcaccg tctcggtccc
     3421 ggtcagcccc agatgcacgt cgcgcccggt gaccatgcca atcagcaata cggcagggat
     3481 cgtgaggccg atcgtcgaca gggcggagcc caggcagaca ttcaccgccc gctgaaggcg
     3541 attggcgagt gcggcatgaa acgccgtgag ggcttccggc gccaagacca gaatggcgat
     3601 caagatgccg ccaagctcgt cgggcaggtc gagatcttcg atgccgtaat cgacgatgac
     3661 cgcgagatat tccgcgagca gcaccacagg tagaagcgac aggatcagca tggccacgtg
     3721 gtacgcgagc gacccggtcg ccgccttcct gtctccacgc ttggggctcg ctgccacagc
     3781 ttcggcgggc tcgtcgaaga aggcgcggtg ccgtagcgtc tgaatagcga ggaacacgcc
     3841 gtagaacagc agcgtgacgg cgaggaacat ccatgctttg ccgggcgcat tgatcgggtc
     3901 cggcggtgtc ttcgtatagt tgggcaggat gagcgcgaag acggaaagcg cggtgatgac
     3961 gacgaggaag gagcgcgcgc cggccaaatt gtagtcttgc tcccaatagc gcagcgcacc
     4021 gatgagaagc gctatgccga ccatgccgtt cagcacgatc atcaaggtgg cgaacattgc
     4081 atcgcgggct agcgtcggat tgttttcgcc gtggatcatg atcgaagcca gcagcgcaac
     4141 ttcgatgacg atgaccgaaa gggtcaggat caaggtgccg tagggctcgc cgagatattc
     4201 ggcaaccgcg tcggcgtggg tcaccacggc gaaggcgcac cacatcatca cgccgaagag
     4261 gaaggcgaac agcacgaccg tgcgcaggtg attgctcagg tccgcaagga atgtgtctcc
     4321 gaatgcgaag aacacaatgg tggtgagaat gccggcgatg gcgggagcaa tgctggttag
     4381 acggaacggc ggcggcgttt tcgcggccgt cttcttcggg acgcgtttgc gtcggcttcc
     4441 ggccgttcgt tctttcatcg ggtctgctcg tgcgggttcg aacaacgcct gacaggcgat
     4501 ttacgggaaa aacctatcac ggcgcctaag cgccttgccc gcgcgcgagc ggccgtcccc
     4561 aactcctttc gacaggcgct agccgccgcc aggcctttga tgtaaaagcc gtttacatga
     4621 ctcactcagg ccgcggctgg cggcgttcag gctcatatca tcacggcaac ctgaaagagg
     4681 tgctgctgga ggcggcgcgc aagctcatcg aagagcatgg cgcgttcggt ttcagcctga
     4741 ccgaggccgc gcggctggcc ggtgtcagcc ccgccgcgcc ctatcgccat ttccgcgatc
     4801 gcgatgcgct ccttgccgag gtcgcccgca gcggcttcga gcgcttcgcc gcgcggctcg
     4861 acgcggcatg gaacaacggc attccgacgc cgctgtccgc gttcgaaaat ttgggccgcg
     4921 ctt
//
LOCUS       DBNHVU010000040         4245 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_980583, whole genome shotgun sequence.
ACCESSION   DBNHVU010000040 DBNHVU010000000
VERSION     DBNHVU010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_980583"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..838)
                     /locus_tag="AC16Y7_01475"
     CDS             complement(137..838)
                     /locus_tag="AC16Y7_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HIR5708080.1"
                     /translation="MGFKDAGKPQYLGHRERLRTRFREAGADAVPDYELLEIVLFRAM
                     PRRDTKPIAKAILARFGTFAKAVNAPEDLLLEIPGLGDAAITEIKLVRTAALRLMRGE
                     VLERPLLGSWKQVLDYCRAAMGFEAKEQFRILFLDKRNKMIADEVQQGGTVDHTPVYV
                     LGSRCRSVHMFAAGGRNDSTLHQAFSAGGIGELLGDDGNSGRPRRHPRLQHQNSRISS
                     DARHCRHENRHAEIL"
     gene            complement(854..1675)
                     /gene="map"
                     /locus_tag="AC16Y7_01480"
     CDS             complement(854..1675)
                     /gene="map"
                     /locus_tag="AC16Y7_01480"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR5708081.1"
                     /translation="MSNLEFARAQVRDTKIKLHDANAFAGMAKACRLVAEMLDLLVDH
                     VKPGVTTDALDAIAYDFAMDHEAIPAPLNYRGFPKAICTSLNHVVCHGIPGPRGLREG
                     DILNIDVTFIVGGWHGDSSRMYPVGKISRAAERLLDVTHNALMRGVAVVKPGATTGDI
                     GHAIQTYAEGERCSVVRDFCGHGLGRVFHDRPNILHYGEPGEGLPLKPGMLFTIEPMI
                     NIGKPHVKILSDGWTAVTRDRSLSAQFEHTVGVTETGYEVFTLSPKGLDRPPAGT"
     gene            complement(1733..2467)
                     /gene="sfsA"
                     /locus_tag="AC16Y7_01485"
     CDS             complement(1733..2467)
                     /gene="sfsA"
                     /locus_tag="AC16Y7_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HIR5708082.1"
                     /translation="MRFSSGLAEGRLLKRYKRFLADVELLSGETVTAHCANPGSMLGL
                     NAPGSRVWLSRSANPKRKLPYSWELIEVDLGVGPTLVGINTGSPNAAVAAAIEAGLIP
                     ALTGYATLRREVRYAENSRIDILLEDPKRPPCYVEIKNVHLMRQAGLAEFPESVTARG
                     TKHLKALCSMVAAGARAVMVYFVQRGDADRFTLAHDIDPTYARAFTEASAAGVEALAV
                     SSEVTLEGLALPRAIPLRPPPGVDEA"
     gene            2620..3396
                     /locus_tag="AC16Y7_01490"
     CDS             2620..3396
                     /locus_tag="AC16Y7_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIR5708083.1"
                     /translation="MTSETSDTVTAALLVIGEEILSGRTRDENVGYIAAYLTRIGIAL
                     REVRVVADVEADIVEAVNALRERYTYVFTTGGIGPTHDDVTTDAIAKAFGVGANVNAD
                     AVAAMRQHYKSGELSPARLRMARIPEGAELIHNAISRAPGYKLQNVIVMAGIPRVMQV
                     MLDEVVPRLAKGRPIISRSVRIDAPEGDIAPPLGALQAAHPEVQMGSYPFFEKNRLGT
                     HVVIRAADEASLDSALSALWTVIAEEGFEAAPADDEPGAS"
BASE COUNT          757 a         1328 c         1357 g          803 t
ORIGIN      
        1 ttgcccgagt gccatatgac cgcgacggcc cgcctcgaaa ttagcggccg gcatgccgtt
       61 caatattgct tgcagcccgc gattgagatc caggttcgca aaaagggcct tggcagcctc
      121 agcgtcgggt atgccgtcaa agaatttcag cgtgccgatt ctcgtgtcga cagtgtctgg
      181 cgtcagaact gattctggaa ttttggtgtt gaagcctggg gtggcgtctt gggcgaccgc
      241 tattcccgtc atcgccgagc agttcaccaa taccgccagc actaaaggcc tgatgtaagg
      301 tggagtcatt tcgtcctccc gctgcaaaca tatgtaccga tctgcacctg gagcctagga
      361 catagaccgg tgtgtggtcc acagtgcctc cctgttggac ctcgtcggca atcatcttgt
      421 tgcgcttgtc gaggaacagg atgcggaatt gctccttggc ttcgaagccc atggcggccc
      481 ggcaatagtc cagcacctgt ttccacgagc caagcagcgg gcgttccagc acctcgccgc
      541 gcatcagccg tagcgccgcg gtgcgcacca gcttgatttc ggtgatggcc gcatcgccta
      601 gccccggaat ctccaaaagc aggtcttccg gcgcgttcac ggccttggcg aacgtgccga
      661 agcgcgccag gatggccttg gcgatcggct tggtatcccg gcggggcatg gcgcggaaca
      721 gcacgatctc cagcagctca tagtccggga cggcatcggc gcccgcctcg cgaaagcgcg
      781 tcctaaggcg ttcgcgatga ccgagatatt gtggtttgcc ggcgtctttg aaccccatga
      841 cgcatcactc gcgtcaagtg cccgccggcg gacgatctag gcccttaggc gacagcgtga
      901 acacctcgta gccagtctcc gtgacgccga cggtatgctc gaactgtgcc gacagcgagc
      961 ggtcgcgcgt caccgccgtc cacccgtcgg acaggatctt cacgtgcggt ttgccgatat
     1021 tgatcatcgg ctcgatggtg aacagcatgc ccggcttcag cggcaggcct tcgccgggct
     1081 ctccgtaatg gagaatgttg ggccggtcgt ggaacacacg cccaagcccg tggccgcaga
     1141 aatcgcgcac cacgctacag cgctcgcctt ccgcataggt ctggatggcg tgcccgatat
     1201 cgccggtcgt cgcgccgggc ttcaccacgg ccacgccgcg catcagcgcg ttatgggtga
     1261 cgtcgagcag gcgttcggca gcgcgggaga tcttgcccac cggatacatg cggctggaat
     1321 cgccgtgcca gccaccgaca atgaaggtca cgtcgatatt gagaatgtcg ccctcgcgta
     1381 gcccccgcgg gcccgggatg ccgtggcaca ccacatggtt cagcgaggtg cagatggcct
     1441 tcgggaagcc gcgatagttc agcggcgccg gaatggcctc atgatccatg gcgaagtcat
     1501 aggcgatggc gtcgagcgca tcggtcgtca ccccaggctt cacatgatcg acgagaaggt
     1561 cgagcatctc cgccacgaga cggcaggcct tggccatgcc ggcgaaggcg ttggcgtcgt
     1621 gcagcttgat cttggtgtcg cgcacttgcg cgcgggcgaa ttcgagattg gacatgggca
     1681 gggattatag ggacttcccg ctagaggtaa gaggaaatct gcgtcggttc aatcaggcct
     1741 cgtccacgcc gggtggcggc cgaaggggaa tggcgcgcgg cagcgccaga ccttcgagcg
     1801 tcacctccga ggagacggcc agcgcttcca ccccggcggc gctggcttcg gtgaaggccc
     1861 tggcgtaggt ggggtcgata tcgtgggcga gggtgaagcg gtcggcgtcg ccccgctgga
     1921 cgaagtagac catgacggcc cgggcgcctg ccgccaccat gctgcagagc gctttgaggt
     1981 gcttggtgcc gcgcgcggtg acggactcgg ggaactcggc aagcccggcc tgccgcatca
     2041 ggtggacgtt cttgatctcc acgtagcagg gcgggcgctt ggggtcctcc aggagaatgt
     2101 cgatgcgcga attctccgcg tagcggactt cccgtcgcag cgtggcgtag ccggtgaggg
     2161 cagggatcag ccccgcttcg atcgccgccg ccaccgccgc attggggctc cccgtattga
     2221 tgccgaccag cgtgggccca acgcccaagt ccacctcgat cagctcccag gaatagggga
     2281 gcttgcgttt cggattggcc gagcgcgaca gccacacacg cgagccgggc gcattaagcc
     2341 ccagcatgga ccccggattg gcgcaatgcg cggtgaccgt ctcgccggag agaagctcaa
     2401 catcggcgag gaagcgctta tagcgtttca ggagccgccc ttcggccagg ccggaggaaa
     2461 accgcatcag catgaggcca cggtacgcga ggtgacgcgc gaccaacaag gtttgcgcga
     2521 tgttccccca cagggcatca aataccggcc aaggacggga caagccggcg aagccggtca
     2581 ataatggcgt ctttcgcccg caaactggct tcaaggatca tgacttcgga gacttcagat
     2641 accgtcaccg ccgcgctgct cgtgatcggc gaggagatcc tctccggccg taccagggac
     2701 gagaatgtcg gctacatcgc cgcctatctc acccggatcg gcatcgcgct gcgtgaggtc
     2761 cgggtggtcg cggatgtgga ggcggacatc gtcgaggcgg tcaacgcgct gcgtgagcgc
     2821 tacacttatg tcttcaccac cggcggcatc gggcccaccc acgacgacgt caccactgat
     2881 gcgattgcca aggccttcgg cgtcggcgca aacgttaacg ccgatgccgt ggccgccatg
     2941 cgccagcact ataagagcgg tgagctctcg cccgcgcggc tccgcatggc ccgcattcca
     3001 gagggcgccg agctgatcca caacgcgatc tcccgcgcgc cgggctacaa gctccaaaac
     3061 gtcatcgtta tggccggcat cccccgcgtc atgcaggtga tgctggacga ggtcgtcccg
     3121 aggcttgcca aggggcgccc catcatctcg cgcagcgttc ggatcgatgc acccgagggc
     3181 gatatcgccc ctccgctggg cgccctccag gcggcacatc cggaggttca gatgggttcc
     3241 tacccatttt ttgaaaaaaa tcggcttgga acccacgtag tgattcgcgc cgcagatgag
     3301 gccagccttg attcggccct gtccgcgcta tggaccgtga tcgccgaaga gggtttcgag
     3361 gcggcgccgg ccgacgatga gcccggcgca agctgacggc acgcgacgcc acgcttcggt
     3421 ctcgtgcatg gctgcatttt tgtctccgcg tcgctctgcg tatctcaaac tccgcagttc
     3481 gaagtccgca tctggcgttc acgcatggtc gaaaccgtta gcgcatcgtg cgcgcggttc
     3541 gctcgagcga cgttgcgtgc aatcgatgca tcaacgatgc aattgtccac aacgccgtcg
     3601 tgacatgcgg caatggcaca acagcattgg atgttttgag acttgcttgc acagcgcgcg
     3661 gcatgtttgt tctgtcgcgc gcaaggttga tgcacggcgc ggcatgtcca ctctgttgcg
     3721 ctgacagtgt ggcgcaaggc gcggtgcgtc aggttgcgtg atgcaattgc agaattaaac
     3781 gagcgctcat tgcagagagg tcagcgcttc gaggtgcacc tgggtttggc ggttggcatg
     3841 aacgcaggtt gccttgcgat ccggttccgc ggattgcctg ttgcttcgcg tcgtagatgc
     3901 ggcgatcagg caacaaaaag ccccggagtt ggccccaggg cagcgcattc taacgtattg
     3961 ataaaaaaga aaaaaggcgc gggagttgcc ccccgcgccc tttgtaaaaa ctagcgcttg
     4021 cgagcgactc tacgcttcgc agtcttgcgc tttgcggcgc cggtcttgcg aacggtccgc
     4081 ttcttggcgg ctgccgtctt acggcgagca gtggtcttcc gcgccgtttt ccggcgaacg
     4141 gtcttccgcg ccgttttccg gcgaacggtc ttccgcgccg ttttccggcg aacggccttc
     4201 cgcgccgtat tccggcgaac ggccttccgc gccgttttcc ggcga
//
LOCUS       DBNHVU010000041         2775 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_312528, whole genome shotgun sequence.
ACCESSION   DBNHVU010000041 DBNHVU010000000
VERSION     DBNHVU010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_312528"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="AC16Y7_01495"
     CDS             complement(<1..851)
                     /locus_tag="AC16Y7_01495"
                     /inference="COORDINATES: protein motif:HMM:NF025080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HIR5708084.1"
                     /translation="MPTQCSENELDLGRSAGRKLVGAFDGGAITSNGGAVLLAGADRG
                     LRLADRLAACFTDLRAADAVKHTLPDLLRQRIFGIALGYEDLIDHDALRFDPVLAAVI
                     DKPGGALAGKSTLNRLEHASKIGQDRHHKLDHDTAAIERLFVDLFLDAHSKPPTRIVI
                     DLDATDDRLYGEQEGRHFHGYYDCYCYLPLYIFCGRHLLAAKLRSSANDVADGATDEV
                     ARIVGHIRELWPRTTIVIRADSGFCRDDLMTWCEENGVQYVLGLAGNARLVSQIASEM
                     KAAERKA"
     gene            complement(1287..2441)
                     /locus_tag="AC16Y7_01500"
     CDS             complement(1287..2441)
                     /locus_tag="AC16Y7_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family RNA-guided transposase"
                     /protein_id="HIR5708085.1"
                     /translation="METTIFVGLDVHKKTIAVATVAADASASVQFYGTIPNTPEAIRS
                     MLRKLAKGGQRLHLTYEAGPLGYNLYRLSTGLGHRCDVVAPSLIPRKAGDKIKTDRRD
                     AMMLAQLLRAGQLTEVWVPDEAHEAMRDLVRMRAQAMKDLRKTRQQLHSFLLRHARVF
                     PGKHWTKAHRRWLGELKFAHPAQHLVLEELLARIERAEELCDRLKASIVELVPQWTLA
                     PVVAAVQALRGVSILVAATVVAEVGNFSRFTSPRQLMAYLGLNPSEHSSGATIKRGAI
                     TKTGNSLARTCLVEAAWTYRHPARVTKIIRDRLEGLPEPIRAIAWKAQVRLSARYRKL
                     VAAGKPAPKAIVAVARELVGFIWAIARLVEPKAAIMEAPKAAIMEAPKAA"
BASE COUNT          486 a          895 c          877 g          517 t
ORIGIN      
        1 ttcgccttgc gctcggcggc cttcatctca gatgcgattt ggcttacgag ccgcgcgttg
       61 ccggcaagcc cgagcacgta ttgcacgccg ttttcctcgc accaggtcat gaggtcatcg
      121 cggcagaagc ccgagtcggc gcggatgacg attgtcgttc tgggccagag ttcgcggatg
      181 tgaccgacga tccgggcgac ctcgtcggtc gcgccgtcgg ccacatcgtt cgccgacgat
      241 ctaagcttgg ccgcaagcag gtgccgaccg cagaagatat agagcggcag atagcagtag
      301 cagtcgtaat agccgtggaa gtgccgcccc tcctgctcgc cgtagagccg atcatcggtt
      361 gcgtcgagat cgatgacgat gcgtgtcggc ggcttggaat gcgcgtccag gaacagatcg
      421 acgaacagac gctcgatggc agcagtgtca tgatcgagct tgtggtggcg gtcctggccg
      481 attttggagg cgtgctccag ccggttcagc gtcgacttgc cggcaagcgc accgcccggc
      541 ttgtcgatga cggcggcgag caccggatcg aagcgcagcg catcgtgatc gatgaggtcc
      601 tcatagccga gcgcgatgcc gaaaatgcgc tggcgcagca gatcgggaag cgtgtgcttg
      661 accgcgtcgg cggcgcgcag atcggtgaag caggccgcca gccgatcagc gagccgcaag
      721 ccccggtcgg caccggccag cagcaccgcg ccaccgttcg acgtgatcgc gccaccatca
      781 aaagcgccca caagcttgcg gcctgcggac cttcccaaat caagttcgtt ctcgctacac
      841 tgtgtcggca tcggggatca tctcctgcaa agcccaagcc tttgttgcag aagaactttc
      901 gctgagtcgg agccccgatg ctcctcaact gtgggagaaa ggcgggctct tatgtccgct
      961 ttgtcaacgt cgaggcgcgc gtcttcgatc cgctttcgcg gcgtggcagt tgatcgatgc
     1021 ccaaggcagg cgacaggacg aaggaacgac ggttgatccg gctcttatcc gcgggttgac
     1081 taccgcatga ccagggttcg tcgcggggct tcctctgtct agaaaacgag cgtgggctgt
     1141 catgttggcg agaagtgcga acgcgacgcc tgctcaaacc cgtatatgcg agggctcccc
     1201 accctctcct gccctgccgc ccgctttggg caccgatatc tcggctgtcg aactgtctcc
     1261 tcttctcgcg ttgatccctg ggttcgtcag gccgccttcg gcgcttccat aatcgcggcc
     1321 ttcggcgctt ccataatcgc ggccttcggc tcgacgagcc gggcaatggc ccagatgaag
     1381 ccgacgagtt cgcgggcgac cgcgacaatc gccttcggcg ccggcttgcc ggcagcaacc
     1441 agcttgcgat agcgcgccga caatctcact tgcgccttcc aggcgatggc ccggatcggc
     1501 tccggaagcc cttcaagcct gtcacgaatg atcttcgtaa cgcgcgctgg atgccggtag
     1561 gtccaggccg cttcgacgag gcacgtgcgc gccaggctgt tgccggtctt ggtgatggcg
     1621 ccgcgcttga tggttgctcc gctcgaatgc tccgacggat tgaggcccag gtaggccatc
     1681 agctgccgtg ggctcgtgaa ccggctgaag ttgccgacct cggcgacaac cgtcgcggca
     1741 accaggatcg agacgccgcg cagcgcctgc acggccgcaa cgaccggcgc cagcgtccat
     1801 tgcggaacca gttcgacgat ggaagccttc aaacgatcac agagttcctc ggcgcgctcg
     1861 atccgagcca gcaactcctc gagaacgaga tgctgagcgg gatgggcgaa cttcaactcg
     1921 ccgagccagc gccggtgcgc cttggtccag tgcttgcctg ggaacacgcg ggcgtgccgc
     1981 agcagaaagc tgtgcagctg ttggcgcgtc ttgcgcaggt ccttcatcgc ctgagcccgc
     2041 atgcgcacga gatcgcgcat cgcctcatgc gcttcgtctg gaacccacac ctcggtcaac
     2101 tgtccggctc tcagcaactg ggcgagcatc atcgcgtcgc gccggtcggt cttgatcttg
     2161 tcgccggcct tgcgcgggat cagcgacggc gccaccacgt cgcagcgatg gccaagcccg
     2221 gtcgacagcc gataaaggtt gtagcccagc ggcccggcct cataggtcag gtgcagccgc
     2281 tgcccgccct tggcgagctt cctcagcatc gacctgatgg cctcgggcgt gttcgggatc
     2341 gtgccgtaaa attgaacaga cgccgaagca tccgctgcaa ccgtcgccac cgcgattgtc
     2401 ttcttgtgga cgtccagccc gacgaaaatg gtagtctcca tcctgatccg ctccccgtgt
     2461 ctggggatag gcctcgctca cgcgaagcaa cccccgttgc gcttcaagcg ccttagtcga
     2521 cacgtcgggg cgggtcacca tccaggtcac ccgtgtcccc agcggacatg atggtctagc
     2581 atctagggaa cctctgaaca gggcggttct catcgggcag gcggttgacg cggttcggtt
     2641 ttggatgccg tcgcggttga atggcgagcg aagtgccgcc gcactggcag cactgccatc
     2701 gatcaggcgc acttcagttg ccgtcttgcc agaaacaggt tggcgagcgc cagcagcgag
     2761 aagacctgcg agccg
//
LOCUS       DBNHVU010000042         3542 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_238669, whole genome shotgun sequence.
ACCESSION   DBNHVU010000042 DBNHVU010000000
VERSION     DBNHVU010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_238669"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..1053
                     /gene="glpX"
                     /locus_tag="AC16Y7_01505"
     CDS             58..1053
                     /gene="glpX"
                     /locus_tag="AC16Y7_01505"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HIR5708086.1"
                     /translation="MDVPDQTARSELDRALALDIVRVTEATAVAAARWRGRGDEAAAD
                     EAAATTMYRAIARLPIEGVVVVGEGEESESPILYIGETLGAASETDVKVDLAVDPIEG
                     TTLCAKALPNSMSVFAVAERGHFLKVPAIYMDKVAIGPGYPDGLINLNVGPTENLKAF
                     ADAKGVPVNELSVCILDRPRHGQLIEEVRNAGAALRLIGDGDIAGVIHVSDPVETGID
                     MYMGTGGAPEGVLAAAALACMGGQMQGRLIASNAEQQARARASGITNLDRTYAIADMV
                     SGDLSFAATGITNGSLLGGVRFTKEAILTQSVVMRASTHTIRWIKTTHADVSKFD"
     gene            1167..2960
                     /gene="recJ"
                     /locus_tag="AC16Y7_01510"
     CDS             1167..2960
                     /gene="recJ"
                     /locus_tag="AC16Y7_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR5708087.1"
                     /translation="MDDAHVLLGVERSATGQRWVERLKAGREHTATAIAQAEDLPELL
                     GRVLAARGAEPQTTRHFLDPSLRTLLPDPSRLQDMERAAQRFARAITEGEPIAVFGDY
                     DVDGAGSVALVERFLRAHEQSCLAYIPDRLTEGYGPSPEALRGLVEEGAKLIVTVDCG
                     TTSAGAISAANTVGAEVIVIDHHQADEDLPPAYAVVNPNRQDDLSGQGHLAAAGVVFL
                     FLVATAGVLRREGFYAGRTEPDLLGLLDLVALATVCDVVPLKDANRAFVAKGLQVLRL
                     RHNAGLRALADTARLDEAPSTYSLGFILGPRINAGGRIGASGLGARLLATEDDVEARA
                     IAAKLEALNAERKSIEDRMLEEAFVRAETVIDASPDAPLIFLGAADWHKGLVGLVAGR
                     IAQRLHRPAFIMGHEPDGTATGSARSVGSVDLGDAVRAAVQEGLLIKGGGHTMAAGFS
                     LALAKEDAVRTFLEERLAGPVAEARAVRSLAIDGALSARGATLHLIDLLDRAGPYGMG
                     HPRPRFAFPSHRIMRLRPIGENHMRFTLQAPDGGRLDAVAFRVAGTPLGDLLAASDGA
                     TLHVAGHLKRSVWQGRESVELMVEDAAPCLG"
     gene            3025..3099
                     /locus_tag="AC16Y7_01515"
     tRNA            3025..3099
                     /locus_tag="AC16Y7_01515"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3058..3060,aa:Glu,seq:ttc)
     gene            complement(3125..>3542)
                     /locus_tag="AC16Y7_01520"
     CDS             complement(3125..>3542)
                     /locus_tag="AC16Y7_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5708088.1"
                     /translation="KYLHTMVRVADVDKSLDFYCNKLGLKEVKRMENEQGRFTLIFLA
                     APGDEDAQVELTYNWDSEEYGEGRNFGHLAYEVDDIYATCDHLMKNGVTINRPPRDGY
                     MAFIRSPDNVSIELLQKGAPKPKQEPWASMENTGKW"
BASE COUNT          599 a         1148 c         1168 g          627 t
ORIGIN      
        1 agttgtgata gggagtctct aagacgtctg tacggacgga cccgcgagtg agggagcatg
       61 gacgtgccag accaaacggc aaggagcgaa ctcgaccgcg cgctcgcgct cgatattgtt
      121 cgcgttaccg aggcaaccgc tgtcgccgcc gcccgctggc gcggccgggg tgacgaggcc
      181 gccgccgacg aggccgcggc caccaccatg taccgggcca tcgcgcggct cccgatcgaa
      241 ggcgtggtcg tcgtgggcga gggcgaggag agcgagtcgc ccatcctcta tattggagag
      301 acgctcggcg ccgcctcgga aacggacgtc aaggtggacc ttgccgtcga tcccatcgaa
      361 ggcacgacgc tgtgcgccaa ggccctgccg aattccatgt cggtcttcgc ggtggccgag
      421 cgcgggcact ttctcaaagt gccggccatc tacatggaca aggtcgccat cggccccggc
      481 tacccggatg gactgatcaa tctcaatgtc ggccccacgg agaacctcaa agccttcgcc
      541 gatgccaaag gcgttcccgt caacgaactc agcgtgtgca tcctcgaccg tccgcgtcat
      601 ggccagttga ttgaagaggt gcggaacgcc ggagcggcgt tgcggttgat cggcgacggt
      661 gacatcgcgg gcgtcatcca tgtgagcgat ccggtagaga cggggatcga catgtatatg
      721 ggcacgggtg gcgcgccaga aggcgtgctc gctgccgcgg ccctggcctg catgggcggt
      781 cagatgcaag gacgcctgat cgcctcgaac gccgagcagc aggcgagggc gcgcgcctcc
      841 gggatcacca acctcgatag gacatacgcg attgctgaca tggtctccgg cgatttgagc
      901 ttcgccgcca cgggcatcac caatggttcg cttcttggcg gcgtgcgttt caccaaggag
      961 gcgatcctga cccagagcgt ggtcatgcgc gcctccacgc acacgatccg ctggatcaag
     1021 accacgcatg ccgacgtctc gaagttcgac tgacgggaga acgggggccc cgcgcgaatc
     1081 ggctacactt ttcgatcctc taagatcgca atcagtgggt agcgtgcgtg acggcagctc
     1141 tcaaaactga cgccaagaac gaaccaatgg atgacgcaca cgtcttgctg ggcgtggaac
     1201 ggtccgccac ggggcagcgc tgggttgagc gcctaaaggc cggccgcgag cataccgcca
     1261 cagccatcgc acaagccgaa gacctgccag agctgctcgg ccgggtgctc gctgcgcgcg
     1321 gtgccgagcc ccagacgacg aggcactttc tggacccgtc gctgcgcacg cttctgccgg
     1381 acccttcgcg tctacaggat atggagcggg cggcgcagag gttcgcgcgc gccatcaccg
     1441 agggtgagcc gatcgccgtg ttcggcgact atgacgtgga cggcgcgggt tccgtggcgc
     1501 ttgtcgagcg cttcctacgc gcccatgaac agtcctgcct cgcctacatc ccggatcgcc
     1561 taacagaggg ctacggaccc tcaccggagg ctctgagggg gcttgtggag gagggggcga
     1621 agctcatcgt caccgtggac tgcgggacga cctccgcggg cgccatttcg gcagccaata
     1681 cggtgggggc ggaggtcatc gtgatcgacc atcaccaggc cgacgaagac ctgccgcccg
     1741 cctacgccgt ggtaaacccg aaccgccagg acgatctctc aggccaaggt catctcgccg
     1801 ccgcgggcgt ggtgttctta ttcctggtcg caaccgccgg ggtcctgcgg cgcgagggct
     1861 tttatgccgg ccgcactgag ccggatctgc tcggtcttct ggacctcgtc gccctcgcca
     1921 cggtttgcga cgtggtgccg ctgaaggacg cgaaccgcgc cttcgtcgcc aaggggctcc
     1981 aggtcttaag gctccggcac aatgccggct tgcgcgcgct cgccgatacc gcgcggctcg
     2041 acgaggcgcc ctcgacgtat agtctcggct tcatcttggg cccgcgcatc aacgccggcg
     2101 gacgcatcgg cgcatcgggg ctgggcgcgc ggttgctcgc aaccgaagac gacgtcgagg
     2161 cacgcgccat cgccgccaag cttgaagcgc tgaacgccga gcgcaaatcc atcgaagacc
     2221 gcatgctcga agaggcattc gtccgcgccg aaaccgtgat cgacgcatcg cccgatgcac
     2281 ccctgatctt ccttggcgct gccgactggc acaaagggct cgttgggctc gtggcgggac
     2341 gcatcgcgca gcgccttcac cggcccgcct tcatcatggg gcacgagcct gatggtacgg
     2401 cgacgggatc ggcacggtcg gtgggctccg tcgatctcgg ggatgcggtc cgtgccgccg
     2461 tgcaggaggg acttctgatt aagggcggcg gccacaccat ggcagcaggg ttttcgctcg
     2521 cgcttgccaa ggaagacgcc gtccgcacct tcttggagga gcgcctggcc gggccggtgg
     2581 cggaagcccg cgcggtgcgg tcgctggcaa tcgacggcgc gctgtctgcc cgcggtgcca
     2641 cgcttcatct gatcgatctg ttggaccggg ccggacccta tggcatgggg cacccgagac
     2701 cgcgcttcgc ctttccgtcc catcgcatca tgcggctgcg gccgatcggc gagaaccaca
     2761 tgcgctttac gcttcaggcg cccgacggcg gccgtctgga cgcggtcgcc ttccgggtcg
     2821 ccggcacccc gctcggggat ctcctggccg cgtcagacgg cgcaaccctt cacgtcgccg
     2881 ggcatttgaa gcgctcggtc tggcagggac gggaaagcgt ggagctgatg gtcgaggacg
     2941 cggctccgtg cctcgggtga gggcaggcgc ctcattcttg cgggaagcgg caaagctttc
     3001 tatatgacgg tgctcaagcg gtacgctccc ttcgtctaat ggttaggacg ccaggttttc
     3061 aacctggaaa caggggttca attcccctag ggagtgccac ttcttctcgg cgcaccgcgc
     3121 ccgcctacca cttcccggta ttttccatcg acgcccaggg ctcttgcttg ggtttcggtg
     3181 cgcccttctg cagcaactcg atcgagacat tgtcgggcga gcggatgaag gccatgtagc
     3241 cgtcccgcgg cgggcggttg atcgtcacgc cgttcttcat caggtggtcg caggtcgcgt
     3301 agatgtcgtc gacctcgtaa gccaggtggc cgaagttgcg gccctcgcca tactcttccg
     3361 aatcccagtt ataggtgagc tcaacttggg catcctcatc gccgggggct gccagaaaga
     3421 tcagggtgaa gcggccttgc tcgttctcca tccgcttgac ttccttcagg ccgagcttgt
     3481 tgcagtaaaa gtcgagcgac ttatcgacat cggcaacgcg caccattgtg tgcagatact
     3541 tc
//
LOCUS       DBNHVU010000043         5163 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1129326, whole genome shotgun sequence.
ACCESSION   DBNHVU010000043 DBNHVU010000000
VERSION     DBNHVU010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1129326"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2349
                     /locus_tag="AC16Y7_01525"
     CDS             <1..2349
                     /locus_tag="AC16Y7_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR5708089.1"
                     /translation="VANMANAIKTISVQRGYDVSEYVMNSFGGAGGQHACLVAEALGI
                     KHVLLHPLSGVLSAYGMGLASLRAARTHAVLTPFEADGLLRLAEISAPLEADARDELV
                     RQGVDASDVTVTAQAHLRYEGTDSSLPVPVGKLTGMRALFETAHQQRFGFISPEKDIE
                     IEAVEVEAVGGGALIEEHDVASSDSTSAPKPSEHADMFTHGQWRKVPVYRRETLARSM
                     RVAGPALIIEPHQTVAIEPAWRADVTAKNHLLLTHAAEGTEAKVGKKADPVMLEVFNN
                     LFVSVAEQMGYALQNTARSVNIKERLDFSCAIFDDEGHLIANAPHIPVHLGSMDRSVE
                     TIQREVGRALKPGDVWMLNAPYNGGTHLPDITVVTPVFAENDTELLFFVAARGHHADI
                     GGIAPGSMSPKATSIHEEGIYIDPFKLVEHGRFREDATCALLTEGPYPVRNLAQNIAD
                     LKAQVAANEKGVIELRKMVRHYELDTVKAYMGHVQDNAADAVRAVIGKLKAARFEVET
                     DQGSVIKVTIGIDRKAKTATVDFAGTSAQGTTTFNAPEPITRACVLYVFRTLVDEDIP
                     LNAGCLRHIKILVPKDSLLKPNYPAPVAGGNVETSQTIVNCLYGALGALGSAQGTMNN
                     LTFGNARFQYYETICSGAPAGPGFDGASAVQTHMTNSRLTDPEVLELRYPVLLECFEI
                     ARGSGGKGEWCAGDGTRRVIRFLEEMDCAILSGFRNVRPFGTKGGAAGQIGENWVRRA
                     GGHLERLKGADQTVIAAGEAVIIKTPTGGGYGTLASKGRRSR"
     gene            complement(2327..2710)
                     /locus_tag="AC16Y7_01530"
     CDS             complement(2327..2710)
                     /locus_tag="AC16Y7_01530"
                     /inference="COORDINATES: protein motif:HMM:NF025838.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4431 domain-containing protein"
                     /protein_id="HIR5708090.1"
                     /translation="MRIAGLLLTLVVMLAVPSAQAASCMRSNADGAIAEGRLSLGQFE
                     DAAGRPEQAFILTLPAPTCLSGEDDMDNVDDVETIHVFSVDALHAGLDEFVGKDVQVR
                     GTPILAHTAHHHAPIVMSITEIDGL"
     gene            complement(2734..3132)
                     /locus_tag="AC16Y7_01535"
     CDS             complement(2734..3132)
                     /locus_tag="AC16Y7_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708091.1"
                     /translation="MHRVNFSAACMISALVLAAPVASAEPTDLKELSKDPQKFLGEQV
                     ELVGFCVKGGRAGDVIGYECTTDAGVYVDIDVIEPESAMKTLADCGATQSDECRATVQ
                     FAPHSYTTSGVIEADKNVTVYHAKKGTVSF"
     gene            complement(3309..3689)
                     /locus_tag="AC16Y7_01540"
     CDS             complement(3309..3689)
                     /locus_tag="AC16Y7_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2852 domain-containing protein"
                     /protein_id="HIR5708092.1"
                     /translation="MHLVATLDDYGKPAWIAATILGFIVWWPIGLGVLAYLIWSGRMG
                     CGNKTDWRQYFTDEARRFGSGGGFSTTGNHAFDEYREETLRRLEDEAKEFQSFLKRLR
                     HAKDKAEFDQFMAEQRPSGTPKAV"
     gene            3905..4678
                     /locus_tag="AC16Y7_01545"
     CDS             3905..4678
                     /locus_tag="AC16Y7_01545"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HIR5708093.1"
                     /translation="MSFAYRRLVGLWVVAALVAGVAFGGPVLARALKDHKPHKGVTRA
                     HTMPIQGIDVSYWQGDIDWRKVREAGIQFAFIKATEGGDHLDPKFLQNWHAAKRAGVA
                     RGAYHFIYWCRLASEQADWFVRNVPRDPDALPPVLDLEWHTHSKTCPKKISRKLALKK
                     TKIILEAMERHTGKRPIIYTDPAFHKDVLKGELKSYHYWLRSVAAEPHKIYGDRSWAF
                     WQFTTTGSVPGIDGHVDRNIYNGSPSDWDRVLRWLEASR"
     gene            complement(4675..>5163)
                     /locus_tag="AC16Y7_01550"
     CDS             complement(4675..>5163)
                     /locus_tag="AC16Y7_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024701.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HIR5708094.1"
                     /translation="DRDALLAEVARSGFERFAARLDAAWNNGIPTPLSAFENLGRAYL
                     AFAREEPASYTVMFESGLAPGTEGENIPAAEQAFDVLQKAAEALCRQLPEDERPPIKL
                     VSLHIWTMAHGVASLFAQEGRAARKVPMTSEEVLESGLLIYLKGLGVLSESEEDGSTL
                     ES"
BASE COUNT          986 a         1628 c         1643 g          906 t
ORIGIN      
        1 gtcgccaaca tggcgaacgc gatcaagacg atctccgtgc agcgcggcta cgacgtctcc
       61 gaatatgtga tgaactcgtt cggtggcgcc ggcggccagc atgcctgcct tgtggccgag
      121 gcgctcggca tcaaacacgt gctgctgcat ccgctgtcgg gcgtgctgtc ggcctacggc
      181 atgggactcg caagcttgcg ggcggcgcgc acgcacgcgg tgctgacgcc gttcgaggcg
      241 gacgggctcc tgagacttgc cgaaatcagc gcgccccttg aggcggacgc gcgcgacgag
      301 ctggtgcgtc aaggcgtgga cgccagcgac gtcaccgtca cggcgcaagc acatctgcgc
      361 tacgaaggca cggacagttc cctccccgtt cccgtgggca aactcacggg catgcgcgcg
      421 ctgttcgaaa ccgcgcatca acagcgcttc ggctttatca gccccgagaa agacatcgag
      481 atcgaggcgg tggaagtgga agcggtgggc ggcggcgcgc tgatcgaaga gcacgacgtt
      541 gcgtcttcgg atagcacatc cgcgccgaag ccgagcgagc atgccgacat gttcacccac
      601 gggcaatggc ggaaggtccc ggtctaccgg cgcgagacgc ttgcgcgcag catgcgtgtg
      661 gccgggcccg cactcatcat cgaaccgcat cagacggttg cgatcgagcc ggcatggcgc
      721 gcggacgtca cggcaaagaa ccatctcctc ctgacacacg ccgccgaggg gaccgaagcc
      781 aaggtcggca agaaggccga ccccgtgatg ctcgaagtgt tcaacaacct cttcgtgtcg
      841 gtggccgagc agatgggcta cgcgcttcag aacacggcgc ggtcggtcaa catcaaggaa
      901 cggctcgact tctcctgcgc catcttcgac gacgaggggc atctcatcgc caacgcgccg
      961 cacatccctg tgcatctcgg gtccatggat cgaagcgtgg agacgatcca gcgggaggtg
     1021 ggccgcgcct tgaagccggg tgacgtgtgg atgctgaacg cgccctataa cggcggcacg
     1081 catctgcccg acatcaccgt ggtgacgccg gtcttcgccg agaatgatac cgagcttttg
     1141 ttcttcgttg ccgcgcgcgg ccatcacgcc gacatcggcg gcattgcgcc gggctccatg
     1201 agccccaagg cgacatcaat tcacgaggaa ggcatctata tcgatccctt caagctggtc
     1261 gagcatggcc gctttcgcga ggacgccacg tgtgcgctcc tcaccgaggg gccctacccc
     1321 gtgcgcaacc tggcgcagaa catcgccgac ctgaaagcgc aggtcgccgc aaacgaaaaa
     1381 ggtgtcatcg agctgcgcaa gatggtgcgc cattacgagc tcgacacggt caaggcttac
     1441 atgggccatg tgcaggacaa cgccgcggac gcggtgcgcg cagtcatcgg caagctgaag
     1501 gccgcgcgtt tcgaggtgga gaccgatcag ggcagcgtga tcaaagtcac catcggcatc
     1561 gacaggaaag ccaaaacagc gacggtcgat ttcgccggca cgagcgcgca aggcacgacc
     1621 acgttcaacg cgccggagcc catcacgcgg gcttgcgtgc tctatgtctt ccgcacgctg
     1681 gtggacgaag acatcccgct gaacgcgggc tgcttacgcc acatcaagat cctggtgccg
     1741 aaggactcac tcttgaagcc gaactatccc gcgcctgtcg cgggcggcaa tgtcgaaacc
     1801 agccagacca tcgtgaactg tctctacggt gcgctcggcg cactgggctc cgcgcaaggc
     1861 acgatgaaca acctcacttt cggcaatgcg cgcttccagt attacgagac aatctgctcc
     1921 ggtgcgccgg ccggcccggg cttcgacggc gcctctgccg tacagacaca catgaccaat
     1981 tcacggctga ccgaccccga agtgctggag ctgcggtacc ccgtgctact ggagtgcttc
     2041 gagatcgcgc gtggctcggg cggcaaaggc gaatggtgcg caggcgacgg aacacgtcgc
     2101 gtcatccgct ttctggagga gatggactgc gcgatcctgt cgggcttccg caacgtgcgt
     2161 cccttcggaa cgaagggcgg cgcggcggga cagataggtg agaactgggt gcggcgcgcg
     2221 ggaggtcact tggaacgcct caagggcgcc gaccagacgg tcatcgctgc cggcgaagcg
     2281 gtcatcatca agacacccac cggcggcggc tacgggacgc tcgcgtctaa aggccgtcga
     2341 tctcggtgat actcatcaca atcggggcat gatggtgcgc cgtgtgcgcc aggatgggtg
     2401 tgccgcgcac ttgcacgtcc ttgcccacga actcgtcgag cccagcgtga agcgcgtcaa
     2461 cggagaagac gtggatggtc tcaacgtcat cgacattgtc catatcgtct tcgccggaaa
     2521 ggcaggttgg cgcgggcaat gtgaggatga aggcctgctc gggacgtccc gctgcatcct
     2581 cgaactgacc gagcgagagc cgcccttcgg cgatcgcgcc atcggcattg gaccgcatgc
     2641 aggaggccgc ctgcgcgctc ggtaccgcga gcatcacgac gagggttagc agcaggccgg
     2701 caattctcat caatcgcccc ccaaatgttc aggctagaag ctcaccgtcc ccttcttggc
     2761 gtggtacacg gtgacgttct tgtctgcctc gatcacgccg gacgtcgtgt aggagtgcgg
     2821 cgcaaattgt accgtcgcgc ggcattcgtc gctttgggtc gcgccgcaat cggccagggt
     2881 cttcatcgcg gactcaggct cgatcacgtc gatatccacg tagacgccgg cgtccgtggt
     2941 gcactcatag ccgatgacat cgccggcgcg cccgcccttc acgcaaaagc cgaccagctc
     3001 gacctgttca ccgaggaact tctgagggtc cttggacagc tccttcaagt cggtgggctc
     3061 ggcggacgcc acgggcgcgg cgaggaccag cgcggaaatc atacaagcgg cggaaaagtt
     3121 cacgcgatgc atttgtctca accttcgttt gttgctggcc ccgggagggg cttgtcggcc
     3181 cgcatcctac agagcgactc acggaaggaa agcctgtctt tcgcgcaaag attaagggcg
     3241 cgggatcgaa atcccgcgcc ccttggaaag cgtggcctca cggccggctc gtccgcaggg
     3301 ggagagcgct agactgcttt cggcgtgccg ctggggcgct gctcggccat gaactggtcg
     3361 aactcggcct tgtccttcgc gtgacgcagg cgcttcagga aggattgaaa ctccttcgct
     3421 tcgtcctcca gccgcctcag ggtctcttcg cggtactcgt cgaaggcatg gttgccggtc
     3481 gtggagaagc cgccaccgct gccaaaccgg cgggcctcat cggtgaaata ctgccgccaa
     3541 tcagtcttat ttccgcatcc cattcgtcca ctccagatga gataggccaa aacgccaagg
     3601 ccaataggcc accacacgat gaaaccgagg atcgttgccg cgatccaagc cggcttcccg
     3661 tagtcgtcta aggtcgcaac taggtgcatc aggtccttcc ttacgtgtga atgttattca
     3721 catttacatt atgttgggat ggttggcaag ttggtcaagg gaacgccgca aatccccctc
     3781 agatatgggg ttgaaacagc cgcccgcgta ggggcagcca aagaaaattt aaccccctgg
     3841 agagatgctt ggccgtcaat tcgctgaaaa ttgcaggcgt gtgtgggtct tcgagggcgt
     3901 ttcaatgagt tttgcgtatc gccgattggt cggtctttgg gtggttgccg ctttggtggc
     3961 cggggtggct tttggggggc ccgtgctggc ccgcgcgctg aaagaccata aaccgcacaa
     4021 gggcgtgacc cgcgcccaca ccatgccgat ccaggggatc gacgtctcct actggcaggg
     4081 cgacatcgac tggcgcaagg tgcgcgaggc cggcatccag ttcgccttta tcaaggcgac
     4141 cgagggcggc gaccatctgg atcccaagtt cctccagaat tggcacgctg cgaagcgcgc
     4201 aggcgttgcc cgcggcgcct accacttcat ctattggtgc cgcctcgcat ccgaacaggc
     4261 cgattggttc gtgcgcaacg tgccgcgcga cccggatgcg ctgccgcccg tgctcgacct
     4321 ggagtggcac acccactcca agacctgccc gaagaagatc tcgcgcaagc tggcgctgaa
     4381 gaagaccaag atcatcctgg aggccatgga gcggcacacg ggcaagcgcc cgatcatcta
     4441 caccgacccg gccttccaca aagacgtgct gaagggcgag ctcaagagct accattattg
     4501 gctgcgttcg gtggccgccg agcctcacaa gatttacggc gaccggagct gggcgttctg
     4561 gcagttcacc accacaggca gcgtgccggg gatcgacggg cacgtcgacc gcaatatcta
     4621 caacggctcg ccgagcgatt gggaccgcgt gctgagatgg ctcgaggcga gccgctagct
     4681 ctcaagcgtc gacccgtcct cctcgctttc ggagagaacg ccgagtcctt tcaagtagat
     4741 gagcagcccg ctctcaagga cttcttcgga ggtcatgggg actttgcgcg ccgccctgcc
     4801 ctcttgggca aacagcgagg cgacgccatg cgccatggtc cagatgtgca ggctgaccag
     4861 cttgatcggc ggccgttcgt cttccggcag ctgacggcat agcgcctcgg cggccttttg
     4921 gagaacgtcg aaggcttgct cggcggcggg gatgttctcg ccttcggtgc ccggcgccaa
     4981 gccgctctcg aacatcaccg tgtagctggc cggctcttcg cgggcgaagg cgagataagc
     5041 gcggcccaaa ttttcgaacg cggacagcgg cgtcggaatg ccgttgttcc atgccgcgtc
     5101 gagccgcgcg gcgaagcgct cgaagccgct gcgggcgacc tcggcaagga gcgcatcgcg
     5161 atc
//
LOCUS       DBNHVU010000044        31393 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_536136, whole genome shotgun sequence.
ACCESSION   DBNHVU010000044 DBNHVU010000000
VERSION     DBNHVU010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 31393)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 31393)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31393
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_536136"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2121)
                     /locus_tag="AC16Y7_01555"
     CDS             complement(<1..2121)
                     /locus_tag="AC16Y7_01555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR5708095.1"
                     /translation="MNEQPKRDRFFGGCPHDCPDTCAMIYEVADGKLVDVRGNKEHPM
                     TRGGLCVKLKDYHDHHYNPDRVLHPLRRVGPKGNRQFERISWDKAIAEITSRWKDIIA
                     KHGSQSIMPYSYLGNEGLVQGLTCGDAFFNRLGSTVNEKTFCASGSSTAWLLTVGPTG
                     GVDPESFVHSKYIVIWACNSISTNLHHWPFVLEAQKKGAKVVVIDSYRSRTAKAADWH
                     IAPKPGTDGALAMGIIAALIRGGYVDDEWVNKHTVGFSELAQRALDFTPDYVEKVTGV
                     AAEDISKFAREFATVQPSVIRMGVALERHAGGGQTIRAVCAIPALAGSWRHVGGGLLQ
                     MPLWEFPVDWGRVSRPDYIRPGTRVVNNLQLGKALMGDMNLDPPIMGLFVYNTNPVSQ
                     APETNRIVQGLSREDLFFVTAEHFVTDTAAYADIILPATMAGEHDDMMFSWGHFYLTL
                     NEKAIEPCGEAKSNAEIFRLLGKAFGFDDDPRFTMSDMELAEHYVQWDAPQMGGIDME
                     HFRQHGYFHLAVGTPDDRLPHAEGNFPTPSGKVEFMVEGAKNFVAPPFRMMYEENQSG
                     EDVDPLPGYLPPRESVETNPNLADQYPLNIISPKSHGFLNSCYANEEHKIKGQGEQFV
                     LISKEDAKARSIRDGDPVRVYNGRGDFEGLARVTDDVNQGVVVATLGYWRSLNRSDGS
                     VNSISSDNFASLGHAPTFSDNLVQV"
     gene            complement(2271..3500)
                     /locus_tag="AC16Y7_01560"
     CDS             complement(2271..3500)
                     /locus_tag="AC16Y7_01560"
                     /inference="COORDINATES: protein motif:HMM:NF013715.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HIR5708096.1"
                     /translation="MSSSKSGTFFLIGPGLLLAATGVGAGDLATGSLVGGILGLAVLW
                     AVVVGAFFKFVVTEGLARWQLATGETLLEGAVSRLGRGVIWCFLPYLLLWSFFVGSAL
                     MSASGVTLYAMLPVFEDAARGKVVFGIAASLIGLAIVWFGGYRVFETVMRFCILIMFV
                     TVVLTAALLWPGTVPVLEGLFIPRIPDAGGEGVKWTLALIGGVGGTLTVLCYGYWLRE
                     KGLTDRQDLRACRIDLVTGYVATAIFGLAMVIVGSTISIDGSGATLLVTLSEQLEALL
                     GPFGKWLFLIGTLGAVFSSLLGVWQSIPYLFADCWRLLERQPSSEVARVDTRAAPYRA
                     YLLLIAIVPIAGLYWRFRDIQLAYAVTGALLFPVLALALLIFNGRWVGPQFRNRPLTI
                     AALVIVLLVFSWMGFRA"
     gene            3632..3919
                     /locus_tag="AC16Y7_01565"
     CDS             3632..3919
                     /locus_tag="AC16Y7_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR5708097.1"
                     /translation="MPFSLAQLIVWLIIGVIAGSLAGTVATAGAGLGRWTSVGVGLVG
                     ALIGGLIFHLFGIWPGLDAISISLRSIVAAFIGSLIFLLLLWIFKRGQGGA"
     gene            4049..5347
                     /locus_tag="AC16Y7_01570"
     CDS             4049..5347
                     /locus_tag="AC16Y7_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708098.1"
                     /translation="MGYCLPIVAVGLASSPLAANESWLEFVGQSEPVEWIPPSQSDNP
                     DAIDPNVDDPYDIVDEGWEPLKIGPVRVGGAFRFNYIYKDWDEDFQGLGELAFDTARI
                     NLDLDDDASPMSGSFEYRYYRDKFADGHDYSMIHHAWIGRKFGERREVQAGITKVPFG
                     ILPYASHNWFFQLPYYLGFEDDYDLGVKFIGDYAPWNIQAAYFARDEGNYFGDSDDSA
                     RYAYDLVREGTNGNAERDQFNGRLAYTMEPREGYSTEIGVSLMVGDISNDNPLGGDGS
                     RYAMAAHLVGNYDRWNLMLQAIHYNFDLNNDPNRDASPNGSFVVLGAYDAEYYVASVA
                     SVYTAGLAYTLPVDWQRIKSLTFYNDFSLMDKQESDFHDSMQNVLGCAVDAEPFYIYI
                     DLAMGKYHPWLGGDWTTALASGGLESDWHTRFNINVGLYF"
     gene            5419..7569
                     /locus_tag="AC16Y7_01575"
     CDS             5419..7569
                     /locus_tag="AC16Y7_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR5708099.1"
                     /translation="MRNISFRATLLTLMLAVLLTAVALMGTAAYLYARFAVQDLGAQV
                     ITQASARVEQHVQHALDVAEEEADTVTTLIQERWLDPENEEHASTYFLAALEARPSLS
                     YLSFGMPSGKYYHALRDRDGGLSVLQLTPEDDGSRRLYEFNVQPDGAPEVVRDIPQSK
                     RTPPYDRPYYRAARDADEAIWTESYVFLGSGESLDVPGVSRAVPVKQADNDSLAGVLT
                     ADFDLHALSRFLRNVTLGDEGFSFLVEVTSDGTPRVIAHPAAADPDPDKRLDLTEPSP
                     DGEGRVTVRANEIKEPRVVKFLDVLGDDLSAVPADLQNVRFETDGRSYIGGFRHLGRE
                     GGPNWIIAMLLPEGEVFGDVQRMARLMLYLGLGGVLMAAVLSVLLSKRIAGSLSAIAA
                     ETREIGQFQLEPKPPIQSRIREIATLAGSVEEMKTGLRSFQKYVPADLVRLLLASGEE
                     AKLGGARKEITIYFSDIVGFTSISESLSPEELVDLLARYLDEMTGEILRNGGTVDKFV
                     GDAIMALWGAPRPHKWDALAACRAALANRTKLAELRQEWAEAGLPALHARTGIHTGPA
                     IVGNFGSPNRLDYTAMGDTVNVASRIEGLNRIFGTEILISEITRKAVDEVMFTRPIDM
                     VAVKGRKEGILIYELIGEARTVSDADRRFAEVYGEALDAYLDRKWDAAHKAFKAAAKL
                     NPEDTATRIMLERCEAYRASPPPPDWDGVFRAPK"
     gene            complement(7583..7671)
                     /locus_tag="AC16Y7_01580"
     tRNA            complement(7583..7671)
                     /locus_tag="AC16Y7_01580"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7635..7637),aa:Leu,seq:taa)
     gene            complement(7819..8769)
                     /locus_tag="AC16Y7_01585"
     CDS             complement(7819..8769)
                     /locus_tag="AC16Y7_01585"
                     /inference="COORDINATES: protein motif:HMM:NF017672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006672 - ceramide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ceramidase domain-containing protein"
                     /protein_id="HIR5708100.1"
                     /translation="MTLGEHVFQYCERGTDTALWAEPLNAVSNAGFFLAALIFWQCLL
                     WRPREQRSADHYLLIALVFLIGLGSLAFHLFATAGTELADVIPITVFMLVYLGVALNR
                     FLSVPPGWTVLLVAGFAGLMAAAAQVHCWDGGIGLAGTVEGSKTCLNGSMSYLPALAA
                     MIIVGMLAAERRHRAAPYVLGAAVVFIVSVLLRSLDFVFCDEVTIDGRDVGTHFMWHL
                     LNAVVIFLLLRATLETGFKEVPLEESGPAPVPALVKAAEPEFEPEPEPEPADEPEPEP
                     EREPEELEDSLSESPEGEEHAEEPEDEGAGESEDEAPPKT"
     gene            complement(8878..10656)
                     /gene="aspS"
                     /locus_tag="AC16Y7_01590"
     CDS             complement(8878..10656)
                     /gene="aspS"
                     /locus_tag="AC16Y7_01590"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR5708101.1"
                     /translation="MHAYRTHTCGALRSDDVGTSVRISGWVHRIRDHGGLLFIDLRDH
                     YGITQIVADPDSPVFAAAETLKPEWVVKIDGKVVARSEDTINPNLPTGQVEVRVEGLE
                     VLSESAELPLPVFGEPDYPEETRLKHRFLDLRRETLHNNVMKRAEIIASLRRRMVEAG
                     FFEFQTPILTASSPEGARDYLVPSRVHPGKFYALPQAPQQFKQLIMMGGFDRYFQIAP
                     CFRDEDARADRSPGEFYQLDLEMSFVTQEDIFETVEPILRALFEEFADGKPVTQDFPC
                     IPYAESMRKYGTDKPDLRNPIEMQDVTDHFRGSGFKIFAGMIEKDANARVWAIPGKGG
                     GSRAVCDRLNAWAQGEGQPGMAYIFYREGEGAGPVAKNVGPERTAAIREQLGLEDGDA
                     VFFVAGLPKEFADFAAQARVKIGDDLKLSATDRFDFCWIVDFPMFEWNEDEQKIDFSH
                     NPFSMPQGGLEALDAIEPEEILAYQYDLVCNGVELSSGAIRNHKPEIMEKAFAIAGYP
                     KEELENRFGGMLRALQYGAPPHGGIAPGIDRMVMLLCGEENLREVVMFPMNQQAEDLL
                     MGAPSEVSQRQLQDLSIRISLPKAKK"
     gene            10931..12100
                     /gene="rnd"
                     /locus_tag="AC16Y7_01595"
     CDS             10931..12100
                     /gene="rnd"
                     /locus_tag="AC16Y7_01595"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HIR5708102.1"
                     /translation="MDLITRTEDLAAACGKLSTASFVTVDTEFMREQTFWPRLCLIQI
                     AGGETEILIDSLAPDLDLQPFFDLMVDESVLKVFHSARQDIEIVHHLAGVVPQPIFDT
                     QVAAMVCGFGEAVSYSMLVKRLLGHNLDKSSRFTDWSRRPLTDRQLTYALGDVTYLRD
                     LYPKLRAQLDKSERASWLSEEMGVLTDPATYEAHPEHAWRRLKMRIKTPKALAILMEL
                     AAWREREAQTQDVPRGRILKDEALYDIAGQAPRTAEDLGSLRSLHNGFARSQRGRAVL
                     ETVQKGLDRDPSTVPPLRRGEPMPPEAQAIVDLLRVLLKATAGRHGVAPKLIATSDDL
                     EQIARSDDADAAALRGWRRKLFGEDALALKRGDLSLAIRKGAVRVLPAEDPEDNA"
     gene            complement(12068..12262)
                     /locus_tag="AC16Y7_01600"
     CDS             complement(12068..12262)
                     /locus_tag="AC16Y7_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HIR5708103.1"
                     /translation="MRAGIIVFYVLLVILLTMASVQYGLGRLPGDIVVDLGDFYFYLP
                     FTTALIVATLLSVVFWIFRR"
     gene            complement(12266..12592)
                     /locus_tag="AC16Y7_01605"
     CDS             complement(12266..12592)
                     /locus_tag="AC16Y7_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR5708104.1"
                     /translation="MPTEHDVVARVHSLTVTRLRIWVSQGLIKPADETARSYSETDIA
                     RADLICTLEDELGFDEEDVPVLLNLIDQIHGLRSELRGLLEVLDELPSDMRSTVRMRI
                     ENRRRG"
     gene            complement(12592..13509)
                     /locus_tag="AC16Y7_01610"
     CDS             complement(12592..13509)
                     /locus_tag="AC16Y7_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017264435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HIR5708105.1"
                     /translation="MAEDLYSELGVSKTAKAGEITKAYRKLAKKLHPDLNPGDKAAED
                     RFKKISHAYSILKDPDKRGQYDRGEIDASGQERSEARYYREHAGGPEGARYRSSAGFE
                     DMGDFSDLFGDMFTGARGGRRGGAQFSMRGQDAQYHLDINFLDAVNGTKTRITLPDGG
                     TLDVTIPPGVSEGQVLRLKGKGMPGMGEGPPGDALVEIGVRGHPVFTREGSDIVVEVP
                     ISFDEAVLGGKVEVPTISGRVAVTVPAGSNSGQTLRLKGRGVKGKGDQLVKLTVMMPE
                     TIHDDLKRFAEDWRKSHAYDPRSKLKEQA"
     gene            complement(13623..15143)
                     /gene="ppx"
                     /locus_tag="AC16Y7_01615"
     CDS             complement(13623..15143)
                     /gene="ppx"
                     /locus_tag="AC16Y7_01615"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HIR5708106.1"
                     /translation="MASRNDRGPVAVVDIGSNSVRLIVYDDAIRSPGPIYNEKVLCGL
                     GRSIASSGKLDTEGVSRALRALRRFRSLADQLKAERIEVIATAAAREAENGPEFIAQA
                     ERILGRQVTVLSGTMEAELAALGIIAGIYAADGFVGDLGGGSLELIDVRGGRLSDAIT
                     MPLGGLRLIDESGGNMKKAREIVDRAFSKLDWLEKGRGRDFYAIGGTWRALARLHMTQ
                     TNYPLRVMHNYRIEADEALKFASLLDHQSQSSLAGIRDISSARRETIPYGALVLERLM
                     KQMKPRAIVVSVLGIREGLLYNLLSDEERAKDPLIEACAYLAKQRSRSQQNAWELSAW
                     TEPLFRMPGPVETPEQTRLRQAACLLSDITWRAHPDYRGAQCLNMVAHGDFVGVDHPG
                     RAFLALAAYYRNEGVIKNDELSQRLVELVDKEALKRARILGAAFRAAHMVTAAMPGVL
                     PHTPLSYEGNRLIWQLPEPYANLEGERVERRFKTLANLLDREPEIRVGAPFQLAAG"
     gene            complement(15148..17328)
                     /locus_tag="AC16Y7_01620"
     CDS             complement(15148..17328)
                     /locus_tag="AC16Y7_01620"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HIR5708107.1"
                     /translation="MNISEQVGPDGAAARIAAFGPDRFYNRELSWLNFNKRVMEEAGN
                     ERQPLLERLRFLSISANNLDEFYMVRVAGLRAQVVAGIETPSQDGLTPTEQLDRINES
                     VADLTANQQETWVTLEALLGEAGIHVLSADQLAGQERTWLEDHFLNHIFPVLTPIAVD
                     PAHPFPFIPNFGFTLGLELVREGSFRTMHALLPIPPQLDRFIRLPAEEGSKEAIRFIR
                     LETVVGIFVSRLFPGYRVRSQGAFRVVRDSDIEVQEEAEDLVRVFESALKRRKRGSVI
                     RLEMEATMPSRLQRFVMQELKVSERESFVQKGLIGLADTNQLIVSDRPDLKFQPFVSR
                     FPERIREHHGDCFAAIAKKDIIVHHPYESFDVVLQFLKQATADPDVVAIKWTLYRTSK
                     TSPIIEALKDAAEAGKSVTAVVELKARFDEEANIKWARDLESAGVQVVYGFIELKTHA
                     KLSLIVRRERGELATYCHVGTGNYHQVTAKIYTDLSFFTADRVVGNDLSRIFNYVTGY
                     AEPAELEDMAVSPHGIRARILDHIREEMYHAKAGRPAAIWMKMNSLVDPEIIDGLYEA
                     SQAGVEIDLVVRGICCLRPGVPGLSDNIHVKSIVGRFLEHSRIYCFGAGHGLPHRKAV
                     VYISSADMMPRNLNRRVEAMTPIKNPTVHEQILDQIMVANLKDNLQSWRVMPDGSSQR
                     IEVEPGQEPFSAQNYFMTNPSLSGRGTSLTENMPPRFTRPVENV"
     gene            complement(17438..18115)
                     /locus_tag="AC16Y7_01625"
     CDS             complement(17438..18115)
                     /locus_tag="AC16Y7_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011424823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR5708108.1"
                     /translation="MSLAQLAFDLPHRAALGAEDFLVSDCNAAAVKLVDAWPQWQDQV
                     QLLTGPPASGKTHLVRVWQGRAGAIALDPENLGIEVIDRLPASMPVAVEDADRAGYDE
                     KSLFHLLNLAREKRLFVLLTSRSSANRWEVGLPDLASRLSAVPVTEIGVPDEALLRTV
                     MLKHFADRQLDIDPKVLEFMAVRIDRSLEAAAAAVEAVDRVALRSGRKISRQLVTETL
                     EGEVSDK"
     gene            complement(18112..18723)
                     /locus_tag="AC16Y7_01630"
     CDS             complement(18112..18723)
                     /locus_tag="AC16Y7_01630"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR5708109.1"
                     /translation="MNIPNTLTLARIVAVPLLVWLIIDNEMLVAFGVFVLAGLSDAAD
                     GFLAKRYGWHTELGAYLDPIADKALLVTIFVTLGLAGHLPVWLVIAVVSRDILIVGAV
                     LLAWMMSRPITVKPLLISKVNTCAQIALAGVVLAELGLGLGLDWLIWLLIWVTGTFTI
                     LSAAAYFWAWLQHMNSYEPEPGAIRRRRRESKVRAPRTRARAS"
     gene            18966..20048
                     /gene="purM"
                     /locus_tag="AC16Y7_01635"
     CDS             18966..20048
                     /gene="purM"
                     /locus_tag="AC16Y7_01635"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008128463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR5708110.1"
                     /translation="MPPQSKSPDPHGKPLTYRDAGVDIDAGNALVEKLGPLAAKTKRA
                     GVLGGLGGFGGLFDLKACGYKDPLLVAATDGVGTKLKLAIETGLHAWIGIDLVAMSVN
                     DLVVQGAEPLFFLDYFSTGALDVGVAETIVGSIAQGCAEAGCALIGGETAEMPGLYEP
                     GDYDLAGFAVGAVERDSLLPRTDLAEGDVLIGLPSSGVHANGFSLVRKVVAASGLGWD
                     DPAPFAPSLKLGQALLTPTRIYVRQLLDTIRGNPGAVKALCHITGGGLIDNLPRVLPA
                     GLTAAIDQSAMPLPPVFEWLQAEARLDPLEMYRTFNCGIGMVAVAAADDATRVRDALG
                     AEAVVIGTLVQRGDGAAVRLDGKRSA"
     gene            20048..20710
                     /gene="purN"
                     /locus_tag="AC16Y7_01640"
     CDS             20048..20710
                     /gene="purN"
                     /locus_tag="AC16Y7_01640"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR5708111.1"
                     /translation="MTKKRVGVLISGRGSNLQALIEAAKAPDYPAKLALVVSNVAGAQ
                     GLARADDAGIPTQVVDHKAFASRPGFEAVLDEALHGAGVDLLCNAGFMRLLTEDFVTR
                     WLNRHLNIHPSLLPAFKGLNTHARVVEAGAKITGCTVHFVRAAMDDGPIVAQAAVPVA
                     PDDTEDSLAARVLAAEHKLYPHALRLVASGDVRVEREHVVGISRPEGENALFSPPLPG
                     TL"
     gene            20963..22327
                     /locus_tag="AC16Y7_01645"
     CDS             20963..22327
                     /locus_tag="AC16Y7_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR5708112.1"
                     /translation="MADMTVAYSPSHGLGEEQSIWFKLTPLLNQENPMQVLAALSDKY
                     NGVIPINLKNQRVVLLSEPEYAERVLVTNADNYTKYFDGLRPIFGRSMITIDGALWQK
                     IRTPQQAAFHPQMFEEYFPYLMSAIDSKMDRWAKFASTGETIEMVEETWTLAAEMICK
                     ALFDREMPFNPHFVFKQVKTYTDVMNHKSIRLKNVRGELVEMTGEDTAKAMEVWHSVP
                     DMVIGADVVEDRDKTLLNMLLEAEADPQFPEFDRRQVVDEIKQYLWAGTETTALTLAW
                     SLYLLSQHPEAMERIRAETRAVCGDADPQWNQVQQLTYTRQVIQETMRLFPPIWALIR
                     IAAGDDDIAGHKIRAGDKIVMLTYIAHHNPKYWDNPEKFDPERFAPERAKKRVKYSYL
                     PFAAGKRSCIGGALSQIENMLALVQLLRRFTPEYVGPNPAKIHATVTLCPKGGLPFRI
                     RELS"
     gene            complement(22334..23035)
                     /locus_tag="AC16Y7_01650"
     CDS             complement(22334..23035)
                     /locus_tag="AC16Y7_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR5708113.1"
                     /translation="MFAVLVAAVSPVGERLAHAADEPAAPCTEDAMLVFDASGSMSGN
                     GWGYGSENPTAVSRIDRVRIALAEILPRITRFRRVGLITYGPGPYQTCNVQLDLSPAP
                     SAAQRILDAVNGLTPAGQTPLSKAVAQAARVLDYRAKPGVIVVLTDGEETCGGKPCDL
                     GKELRKDGLQLTVHVIGLRVKGITWTGEQAVVETRCLAEETGGLYTTVETEEELTDAL
                     EKTLGCPMVTEALKR"
     gene            complement(23109..23732)
                     /locus_tag="AC16Y7_01655"
     CDS             complement(23109..23732)
                     /locus_tag="AC16Y7_01655"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5708114.1"
                     /translation="MPRMLLLVSVLAGLLALPAITSRAEDVPPDANGVPKDIPKTQSP
                     ALDWGMRNLDPDQRQRMLRFSTFVNGGIPDDYQKAENTVGYTVRAISEGGQLYASHCQ
                     KCHGEWGLGNGDLAYALKPSPALLAYMVQQPVAVDQYLLWSIAEGGAPFGTAMPGFKD
                     QLSHEQIFQIVAYLRAGFPDLDDKPPAKRGPGAAATDEPDPRTHTID"
     gene            complement(23797..24219)
                     /gene="ndk"
                     /locus_tag="AC16Y7_01660"
     CDS             complement(23797..24219)
                     /gene="ndk"
                     /locus_tag="AC16Y7_01660"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIR5708115.1"
                     /translation="MASERTMSIIKPDATERNITGLIIARLEGAGLRVIAQKRVWWKK
                     KDAQKFYEIHKDRSFYKDLIQFMTSGPVVLMVLEGEDAIARNRSVMGRTNPVDAEPGT
                     IRKDFGVNMQRNSVHGSDSPESAQREIDLCFKKAEIIG"
     gene            24366..24716
                     /locus_tag="AC16Y7_01665"
     CDS             24366..24716
                     /locus_tag="AC16Y7_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HIR5708116.1"
                     /translation="MLYATIKVLLTSVLVVAVSEAAKRSALFGAIIASIPLTSVLAMI
                     WLYIDTGDTEKVARLASGIFWLVLPSLVLFLTLPLLLRAGVDFYASLAASVTLTVGAY
                     FAMIYGLRLAGIEI"
     gene            complement(24713..25171)
                     /locus_tag="AC16Y7_01670"
     CDS             complement(24713..25171)
                     /locus_tag="AC16Y7_01670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HIR5708117.1"
                     /translation="MSGETEVYFYHLEGRTLEQVLPTLLERSLERGWRAVVQASSPER
                     VEALNTLLWTFREDSFLPHGTSSDGTAAMQPIFLTVEADNPNDAAVRFLVDGATLDDA
                     SPYARVVYVFDGHDDDAVSQARAAWTEAKDKGFAVSYWQQDGEGRWQQKA"
     gene            complement(25175..26527)
                     /gene="der"
                     /locus_tag="AC16Y7_01675"
     CDS             complement(25175..26527)
                     /gene="der"
                     /locus_tag="AC16Y7_01675"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR5708118.1"
                     /translation="MSFTVAIVGRPNVGKSTLFNRLAGKRLALVDDQPGLTRDRREAE
                     TVLGACAVTLVDTAGLESGDEGLSARMRQQTDEAIAQADLVLFLIDARVGITAADEIF
                     GELVRASGKPVVLAANKSEGRAAEAGYYEAFALGLGEPLAISAEHDLGIGEIAEAIEQ
                     ACSARKPEPDTDAVEADSGPHPLRIAVVGRPNVGKSTLVNALLGEERMITGPEAGITR
                     DAIASEIEWNGRPMRVFDTAGLRRKARIEKKAEVLSVGDTLKAIRFAEVVILLLDAEQ
                     PFEKQDLQIADLIAQEGRALVIAINKWDLVEDRQQRLAELREMCQRLLPQVKGVSLVT
                     LSANSGKGLGRLMRAVVAADEMWNRRLPTHTLNQWLRGATEAHPPPAVSGRRIKLRYM
                     TQANARPPTFVVFCSLPKALPDAYVRYLVNGLRETFDLPGVPIRLNLRKGENPYVKGR
                     "
     gene            complement(26583..27893)
                     /locus_tag="AC16Y7_01680"
     CDS             complement(26583..27893)
                     /locus_tag="AC16Y7_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR5708119.1"
                     /translation="MGYRLLGAGLTVSLAIVLTACGSLSAIKNPFGEEEERLPGERVS
                     VITDPGQFNAAQISPRAVALPPARSNASWAQPGGSASNNLGHLALAGGLRKAWSVDAG
                     TGSSSRGRLSAVPIVAGGRVYTLDAAGNVSAFSASSGAKAWGVSVTPDNESSKEGFGG
                     GLAFESNTIYVTTGYGTVVALNASSGAVLWTTPIGEPIRNSPTVAGGKVFFVSTENTL
                     HALNASTGSKLWRARGLPQPTTLLSNASPAVASGTVVAPFPAGDIMAFQTGSGKRAWS
                     DSLSRSSDTSAAGILGDPARPVIDRGVVYAVSHGGRMIATSAGSGARVWTRNIASTQM
                     PWLAGDVVFVVDTRGRLMAIGRRDGQIRWVSDLPASSRWSGPVLAGSRVWVVSGDGLL
                     VGADARTGQLGTQIDLDTDVFVTPVVAGGRMYILSDDADLIALN"
     gene            complement(27960..28643)
                     /locus_tag="AC16Y7_01685"
     CDS             complement(27960..28643)
                     /locus_tag="AC16Y7_01685"
                     /inference="COORDINATES: protein motif:HMM:NF021492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5708120.1"
                     /translation="MSDNSFFREVDEAVRHERYKALWDQYGVYIIILAAAVIAGVAGY
                     KGWTYWTERQAKEAGTEYTQALALKTGADLDKAREAFETLAKDGPSGYQVLSRFQLAA
                     TDAKAGDTDKAVAAYDALATDAGVDPILQGLATVQAATLRVDAADYAEMERRLNGLVE
                     NNSRWRFSARELLGLSAYQHKDMRAAEEQFSALLADQNTPPNLRERANMMLALIVSST
                     PAQSSSTTN"
     gene            complement(28797..29666)
                     /gene="panB"
                     /locus_tag="AC16Y7_01690"
     CDS             complement(28797..29666)
                     /gene="panB"
                     /locus_tag="AC16Y7_01690"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR5708121.1"
                     /translation="MSQSFDVKRVTAPQIAGRKGGAPIVALTAYHAHTAQIIDPHVDL
                     LLVGDSLGMVMHGYETTVPVPLDLMIMHAQAVVRGSKHALVVVDMPFGSYEESPAVAF
                     RNAARIMKETGAGAVKIEGGVHMAETIDYLTSRGIPVMGHIGLTPQAVNQIGSFKPQG
                     RHRSEWAPFEEDAKAVAGAGAFAVVLEALAELLAARITKQISIPTVGIGASAECDGQI
                     LVLEDMLGLSPRVPKFVKEYAQLGDAIEGAVKAYSADVRRRAFPSDAHTYPMIDEDGG
                     DDEDTKKTEMTGS"
     gene            complement(29760..30332)
                     /locus_tag="AC16Y7_01695"
     CDS             complement(29760..30332)
                     /locus_tag="AC16Y7_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0090471 - 9,15,9'-tri-cis-zeta-carotene
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0016120 - carotene biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HIR5708122.1"
                     /translation="MTLLIIGIVVFLGIHLVPAFPHVREALIGRLGEKGYQVAFALAS
                     IAGFALLVWGFAVAPFVQVWPPPAWTRWVAIALMLPAFIFLFAAYIPGGIKAKLKHPY
                     LVAIKTWALAHLIANGDLASIILFGSFLAYAIFDRISLKRRQPRELIVSAAPGGARND
                     VIAVVLGGVFYVIFLIWLHPLLIGRAVVPH"
     gene            complement(30523..31257)
                     /locus_tag="AC16Y7_01700"
     CDS             complement(30523..31257)
                     /locus_tag="AC16Y7_01700"
                     /inference="COORDINATES: protein motif:HMM:NF015961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR5708123.1"
                     /translation="MGNSWIRRQKVRLRRPAQILAAPVFSNYVKLVLGTSSRVSDPPD
                     YVARAWENHPQIIALWHGQFFLLPGIYPQEVPGRAMVSRHEDAEALARVLRGFGLELI
                     RGAGAGERRRDRGGAEAAHGAVASLREGYSIAMTADVPPGPARKCGRGIVTIASMSGR
                     PVVPFAVASSRYAAVNSWDRMTFNLPFSKLGIAMGDPIQVPPDLNVRELEPYRQQVED
                     ALNVTTEKAYALAGADSARATPPKRR"
BASE COUNT         5929 a        10190 c         9619 g         5655 t
ORIGIN      
        1 tacctggacg aggttgtcgg agaatgtcgg cgcgtgcccc aaactggcaa agttgtccga
       61 gctaatcgag ttcaccgatc cgtccgagcg gttgaggctg cgccaataac cgagcgtcgc
      121 cacgaccacg ccctggttca catcgtccgt gacgcgcgca agtccctcaa agtcgcctcg
      181 gccgttgtat acgcgcacgg gatcgccatc gcggatcgaa cgagccttcg catcctcttt
      241 gctgatcagc acgaactgct cgccctggcc tttgatcttg tgctcttcgt tggcgtagca
      301 cgagttgagg aagccgtggc tcttcggcga tatgatattg agcgggtatt gatcggcgag
      361 attgggattg gtctccacgc tctcgcgcgg cggcaaatat cccggcagcg ggtcaacgtc
      421 ctcgcccgac tggttttcct cgtacatcat gcggaacggc ggcgcgacga agttcttcgc
      481 gccttcgacc atgaattcca ccttgccaga cggcgtcggg aaattgcctt cagcatgagg
      541 caggcggtcg tccggcgtgc cgacggcgag atggaaatag ccgtgctgcc ggaagtgttc
      601 catgtcgatg ccgcccatct gcggcgcgtc ccattgaacg tagtgctcag cgagttccat
      661 gtcgctcatg gtgaagcgcg gatcgtcgtc gaagccgaac gccttgccaa gtaagcggaa
      721 gatctccgca ttggacttcg cctcgccgca cggctcgatc gccttctcgt tgagcgtcaa
      781 atagaaatgc ccccaggaga acatcatgtc gtcgtgctcg ccggccatcg tcgcgggcag
      841 aatgatgtcc gcataggccg ccgtgtccgt cacgaagtgc tcggcggtga cgaagaacaa
      901 atcttcgcgg gagagcccct gaacaatacg attggtttcc ggcgcctggc tcacggggtt
      961 cgtgttgtag acaaaaagcc ccatgatcgg cgggtcgaga ttcatgtcgc ccataagcgc
     1021 cttgccgagc tgcaaattgt tcaccacgcg ggtgccaggc cgaatgtagt cgggacgcga
     1081 gacacgtccc caatccaccg ggaattccca gagtggcatt tgcaggaggc cgccgcccac
     1141 atggcgccat gacccggcaa gcgccgggat cgcgcacacc gcgcgtatgg tctgcccacc
     1201 gcccgcgtgc cgctcgagcg cgacacccat gcggatcacc gatggctgca ccgtggcgaa
     1261 ctcgcgggcg aacttcgaga tgtcctcagc cgcaacgccg gtgaccttct ccacatagtc
     1321 cggcgtaaaa tcgagcgcgc gttgggcgag ttccgagaac ccgaccgtgt gtttgttgac
     1381 ccactcgtca tcaacatacc caccgcggat cagcgccgcg atgatgccca tggcaagtgc
     1441 accgtccgtg ccgggcttcg gcgcgatgtg ccagtcagcc gccttcgccg tgcgtgaccg
     1501 atagctatcg atcaccacga ccttggcgcc cttcttctgg gcttcgagca caaacggcca
     1561 gtgatgcaag ttggtggaga tcgagttgca ggcccaaatg acaatgtact tggaatgcac
     1621 gaaactttcg gggtcgacgc cgcccgtggg cccgaccgtc aacagccacg cggtcgaaga
     1681 tcccgaagcg cagaacgtct tctcgttcac tgtggagcca agccggttga agaacgcatc
     1741 gccgcaggtg agcccctgca ccagcccttc attgccgaga tagctgtacg gcatgattga
     1801 ttggctgccg tgtttggcga tgatgtcctt ccagcgcgat gtaatctcgg cgatcgcctt
     1861 gtcccaagag atgcgctcga actggcggtt ccccttcggg ccaacgcgcc gcaagggatg
     1921 gagcacgcgg tcgggattgt agtggtgatc gtgatagtcc ttgagcttca cgcacagccc
     1981 accccttgtc atcgggtgtt ccttattgcc ccggacgtcg acgagcttgc cgtcagcaac
     2041 ctcgtagatc atggcacaag tgtcgggaca gtcgtgggga caccctccga aaaacctgtc
     2101 gcgcttgggc tgttcattca tgctggctcc tccctcatcc cgtctagcgg ctcttgatgg
     2161 ccactgatct gcatcagagt acgaaggtcg gagacctttg tcgattcagc accggcacgg
     2221 attggcgatg ggcgcccaac gtcctaattt tccgcaggaa agcgcagccc ttaagcgcga
     2281 aagcccatcc agctgaagac cagcaggaca atgacaaggg ccgcgatggt aagcgggcgg
     2341 ttgcgaaact gcggaccaac ccacctgccg ttgaagatca ggagagcgag cgccaggact
     2401 ggaaacagca gcgcgccggt aacagcgtag gccagttgga tatcgcgaaa gcgccaataa
     2461 agtcccgcta tgggcacgat agcgatgagc agaaggtaag cccgatacgg cgctgcgcgc
     2521 gtgtcgacgc gggcaacctc gctgcttggc tggcgctcca gcaagcgcca gcaatcggcg
     2581 aagagatatg ggatggactg ccacacgccg agaagacttg agaacaccgc gcccagcgtg
     2641 ccgatgagaa acagccactt gccgaacggc ccgagcagtg cctcgagctg ctccgacagc
     2701 gtcacgagca acgtcgcgcc gctgccgtcg atactgatgg tcgaaccgac gatgaccatt
     2761 gctaggccaa agattgccgt cgccacatag cccgtgacga gatcgatgcg gcatgcgcgc
     2821 aagtcttgcc tgtcggtcag ccctttttcg cgcaaccaat agccgtaaca aagcacggtg
     2881 agcgtcccgc cgacgccgcc gatcagagcg agcgtccatt tgaccccctc cccgcccgca
     2941 tccggaatgc gcgggatgaa tagcccctcc agaacgggga ccgttcccgg ccagagcagc
     3001 gcggccgtca gcacgacggt gacgaacatg attagaatgc agaaacgcat gacggtctca
     3061 aacacgcgat agccgccgaa ccaaacgatg gcgagaccga tcaggctcgc ggcgatgccg
     3121 aacacgacct tgccgcgtgc ggcgtcctcg aagaccggca gcatcgcgta aagcgtcacg
     3181 ccactcgcgc tcatcaaggc cgagcccacg aagaaggacc acagcaggag ataaggcaga
     3241 aagcaccaaa tcacgccgcg gcccagacgg ctgacagcgc cttccagcag cgtttcgccg
     3301 gtcgccaatt gccaccgcgc cagaccttct gtcaccacga acttgaagaa cgcaccgacg
     3361 accacggccc aaaggacggc cagtcccaaa atcccgccaa cgagactgcc cgtggcgaga
     3421 tcgccggcac cgacaccggt cgccgcaagc agcagcccgg gcccaatgag aaagaatgtt
     3481 ccgctcttgg aagacgacat ccggcgcaaa ctagcgggtg caggcggcgg gagtcgaatt
     3541 cggtgcggct ctcctataag ctaagctttc gcacgaccgt tcaaatgaaa ccggtcattc
     3601 gacgcatttg cccttgggga ggctacaaga gatgccattt tcgctcgctc aactcatcgt
     3661 ttggttgatc ataggcgtga tcgctggctc gctggcagga accgttgcga cagcaggcgc
     3721 cggtctaggc cgttggacca gcgttggtgt cggtctggtc ggcgcattga ttggcggcct
     3781 catttttcac ctattcggga tctggccagg cctcgatgcg atttccatct cacttaggag
     3841 catcgttgca gcctttatcg gctcactgat ctttctgctg cttctttgga tcttcaaacg
     3901 cggacagggc ggcgcctagt ctgacatcaa tcgctctgtc tggttgggag cgactccttg
     3961 gctactcgtt tggccattag ccgacgttga gggggcttgc cgaaggttgg taatgaagtt
     4021 ggctacggca gtccggttgc ttagcggggt gggatattgt ctcccgatcg tcgccgtggg
     4081 gttggcatcg tcgccccttg ccgccaacga atcttggctc gaattcgtcg gacagtccga
     4141 acctgtcgaa tggatcccgc cgtctcagag cgacaatcca gatgcgatcg atccaaacgt
     4201 tgacgatccc tacgacattg tcgacgaggg ctgggagcct ctcaagattg gacctgtgcg
     4261 ggtcggcggc gccttccgct tcaactacat ctacaaagac tgggacgagg actttcaagg
     4321 actcggcgaa ctggccttcg acaccgcgcg gatcaatctc gacctggatg acgatgcaag
     4381 tccgatgtcc ggctccttcg aataccggta ctaccgggac aagttcgcgg acggtcacga
     4441 ctattcgatg atccatcacg cctggatcgg gcggaagttc ggcgagcggc gcgaagtaca
     4501 ggccggcata acgaaggtcc ccttcggcat cctgccttac gcatcgcaca actggttctt
     4561 tcagttgccg tactatctcg ggttcgaaga cgactacgat ctcggcgtga agttcattgg
     4621 cgactacgcg ccctggaaca ttcaagcggc atactttgca cgcgatgagg ggaactactt
     4681 cggcgacagc gacgacagcg cgcgctacgc ctacgatctc gtgcgcgagg gcacgaacgg
     4741 caatgccgaa cgtgaccagt tcaatggtcg cttggcctac acgatggaac ctcgggaggg
     4801 gtacagcacg gagatcggcg tttcgctgat ggtcggagac atatcgaacg acaacccgct
     4861 aggaggcgat ggaagccggt atgcgatggc cgcgcatctc gtcgggaatt acgaccgttg
     4921 gaatctcatg ctccaggcga tccactacaa ctttgacctg aacaacgacc caaaccgcga
     4981 cgcgagtcca aacggaagct tcgtcgtcct aggcgcatac gatgcggagt attatgtggc
     5041 ctcggtcgcg agcgtctaca ccgcaggact tgcgtatacc cttccggtcg attggcaacg
     5101 catcaagtcg ctcaccttct ataatgactt cagcttgatg gataagcagg aaagcgactt
     5161 tcacgacagc atgcagaacg tgctcggctg cgccgtagac gccgaaccgt tctatattta
     5221 catcgacctc gccatgggca agtaccaccc ctggcttggc ggagattgga ccaccgccct
     5281 tgccagcggc ggcctggaaa gcgactggca cacgcgcttc aacatcaatg ttgggctcta
     5341 cttctaagcc catggtgtgg agtatcctca taaattgcgg cttgggccgg aacaggctcc
     5401 tcggaaggtt cccacatcgt gcgcaacatt agcttccggg ccactctcct caccctcatg
     5461 ctggccgtgc tgctcactgc ggtggcactg atggggacgg ccgcctatct gtatgcgcga
     5521 ttcgctgtgc aggacctcgg cgcacaagtg atcacgcaag cgtccgcgcg cgtcgaacaa
     5581 catgtgcagc atgcgttgga cgttgccgag gaagaggcgg acacggtcac cacgctgata
     5641 caagaacgct ggctcgatcc cgaaaacgaa gagcacgcca gcacctactt ccttgccgcg
     5701 cttgaggcac ggccgagcct gtcctacctg tcgttcggca tgccgagcgg aaagtactat
     5761 cacgccctcc gcgaccgcga tggcgggctc agcgtcttgc agctaacgcc cgaggacgat
     5821 ggaagccgcc gcctctacga attcaatgtt cagcctgacg gcgcgccgga agtggttcgc
     5881 gacattccac agtccaagcg caccccgccc tatgaccggc cctactaccg cgcggcgcgc
     5941 gatgcagacg aggcgatctg gactgagtcg tatgtgttcc tcggatccgg agagtcgttg
     6001 gacgttccag gggtgagccg tgccgtgcca gtgaagcaag cggataacga cagcctcgcc
     6061 ggcgtgctga ccgccgattt cgatctccac gcgttaagcc gcttccttcg caatgtcact
     6121 ttgggggatg aagggttctc cttcctggtg gaggttacat cggacggcac cccacgggtt
     6181 atcgcccacc cggcggcagc ggaccccgat cccgacaagc ggctcgacct gaccgaaccg
     6241 tctccggacg gcgaaggccg cgttaccgtg cgcgccaacg agatcaaaga gccgcgcgtg
     6301 gtcaagttcc tcgatgtgct tggtgacgat ctctccgcgg tgccggcgga tctccagaac
     6361 gtacgatttg aaaccgatgg ccgttcatat atcggcggct tccggcatct cggccgcgag
     6421 ggaggcccga actggatcat cgccatgttg ctgcccgagg gcgaagtctt cggcgacgtt
     6481 cagcgcatgg cgcggctcat gctgtatctc gggctcggcg gtgtactcat ggctgccgtg
     6541 ctctccgtgc tgctctcaaa acgcattgcc ggaagtttga gcgcaatcgc cgcggagacc
     6601 cgcgagatcg gacaattcca actggagccg aaacccccga tccaaagccg catccgagaa
     6661 atcgccacgc tggccggctc ggtcgaggaa atgaagacgg gcctgcgatc cttccagaaa
     6721 tacgtgcccg cagacctggt gcgcctactg cttgcgtccg gggaggaagc caagctcggc
     6781 ggcgcccgga aggagatcac gatctacttc tccgacatcg tcggattcac ctcaatctcc
     6841 gaaagccttt cccccgaaga gcttgtcgac ttgcttgcgc gctatctcga cgagatgacg
     6901 ggcgaaatcc ttcgcaacgg cgggacagtc gacaagtttg tcggtgatgc aatcatggcc
     6961 ctttggggcg caccccgccc tcacaaatgg gacgcgctgg cggcgtgccg tgcggcactt
     7021 gccaatcgta cgaagctggc ggaactccgc caagagtggg ccgaggccgg cctgcccgcg
     7081 ctccacgcgc gtaccggcat ccacaccggg ccggccatcg tcggcaattt cggcagcccc
     7141 aaccgcctcg actacacagc catgggagac acggtcaacg tcgccagccg gatcgaaggt
     7201 ctcaaccgga ttttcggcac ggagattttg atcagcgaga tcacgcgtaa agcggtcgat
     7261 gaggttatgt tcacgcgccc gatcgacatg gtcgccgtca aaggacgcaa agaaggcatc
     7321 ttgatctatg agttgatcgg cgaagcaagg accgtctcgg atgcggatcg gcgtttcgcg
     7381 gaagtctatg gcgaggcgct cgatgcgtat ctggaccgca agtgggacgc ggcccataag
     7441 gcattcaagg ctgccgcgaa gctgaacccc gaagacaccg caacgcgcat catgctcgag
     7501 cgttgcgagg cgtaccgcgc ctcaccccca ccgccggatt gggatggtgt cttccgcgcg
     7561 ccgaagtaag cgagggaaca ggtggtgcgg acggcgggag tcgaacccgc acgggggttg
     7621 cccccctcag gattttaagt cctgtgtgtc taccagttcc accacgtccg ctgacgccaa
     7681 accacgtctg acgatagcgc ctgagccatc tgtatagacg gagtctttgc gcgagggaaa
     7741 agcccttaga ccggaaagga cagcttctcc cgcgaagggc ctggacggat aaggcccggc
     7801 cggagctgaa agaaacgttc aggtcttggg tggcgcctcg tcctctgact cacccgcgcc
     7861 ctcgtcctct ggctcctccg catgttcctc accctcaggc gactcggata atgagtcttc
     7921 tagctcttcg ggctctcgct ccggctcggg ttccggctcg tccgcgggtt ccggttccgg
     7981 ttcgggctca aactcaggtt ctgccgcctt cacgagagcc ggaactggcg caggtccgct
     8041 ttcctcaagc ggaacctcct tgaaccccgt ctccagggtc gcgcgcaaca gaaggaagat
     8101 caccaccgcg ttgagcaaat gccacatgaa gtgcgtaccg acgtcgcgcc catcgatcgt
     8161 cacctcatcg cagaagacaa agtcgagcga gcgcaggagg accgagacga taaagaccac
     8221 cgcggcgccg agaacataag gcgcggccct gtggcgacgc tcggccgcca gcatgccgac
     8281 gatgatcatc gcggcgagcg ccggcaaata ggacatgctc ccgttgaggc aggtcttgga
     8341 gccttccacc gtacccgcaa ggccaatgcc gccgtcccag caatgcactt gcgcggccgc
     8401 cgccatgagc ccggcgaagc ccgccacgag cagcaccgtc cagccgggcg gcacgctcaa
     8461 gaagcggttc aacgcaacac cgagatagac cagcatgaac acggtgatgg ggatcacgtc
     8521 ggccagctcc gtgcctgcgg tcgcaaagag atggaacgcg aggctgccga gcccaataag
     8581 aaagacgagc gcgatgagga gatagtggtc cgcgctgcgc tgctcgcgcg gccgccagag
     8641 caggcactgc caaaagatca gcgcggcgag gaagaagccg gcgttggaga cggcgttaag
     8701 cggttcagcc caaagcgccg tgtcagtgcc gcgctcgcaa tattggaaca cgtgctcgcc
     8761 aagagtcatg gccccttttc ctttcgaccc gtgaactagg aaagaaccct tcccgccggc
     8821 gaacattccg cgttgtcatg caactgacgc gaaacgcgcc agacacaaga cggttcgcta
     8881 tttttttgct ttaggcagag aaatgcggat gctgaggtcc tgaagctgtc tttgcgacac
     8941 ttcggacggc gctcccatga gcaggtcttc ggcctgctgg ttcatgggga acatgaccac
     9001 ctcgcgtaag ttctcctcgc cgcacagcag catgaccatg cggtcgatac ccggcgcgat
     9061 gccgccatgg ggcggtgcgc cgtattgcag cgcgcgcaac atgccgccga agcgattctc
     9121 gagctcttct ttgggatagc cggcaatggc gaaggccttc tccatgatct ccggcttgtg
     9181 gttccggatc gcgccggagg acagctccac accgttgcac acgagatcgt actggtaagc
     9241 caggatctcc tcgggctcga tggcatcgag cgcctcaagt ccgccttgcg gcatggagaa
     9301 cgggttgtgg gagaaatcga tcttctgctc gtcctcgttc cactcgaaca tggggaagtc
     9361 gacgatccag cagaagtcga agcggtccgt cgccgagagt ttcagatcgt cgcctatttt
     9421 gacccgcgcc tgcgcggcga agtcggcaaa ctcttttggg agcccggcaa cgaagaacac
     9481 cgcatcgccg tcctcaaggc caagctgctc gcggatcgcc gcggtgcgct ccggcccgac
     9541 attcttggcc accggccctg caccttcgcc ctcacggtag aagatatagg ccatacccgg
     9601 ctggccctcg ccttgcgccc aggcattcag gcggtcgcat accgcgcggg agccgccgcc
     9661 cttgccgggg atcgcccaga cgcgcgcatt cgcgtccttc tcgatcatgc ccgcgaagat
     9721 cttaaagccg gagccgcgga agtggtccgt gacgtcctgc atctcgatcg ggttgcgcaa
     9781 gtccggcttg tcggtgccgt atttgcgcat ggactccgcg tacggaatgc acgggaagtc
     9841 ctgcgtcact ggcttgccgt cggcgaactc ctcgaacagc gcccgcaaga tcggctcgac
     9901 cgtctcgaag atgtcttctt gggtgacgaa gctcatctcc agatcgagct ggtagaattc
     9961 gcctgggctg cggtcggcgc gcgcatcctc gtcgcggaag cacggcgcaa tctggaagta
    10021 gcggtcgaag ccgcccatca taatgagctg cttgaattgc tggggtgcct gcggcagcgc
    10081 atagaatttg cccggatgca cgcgggacgg cacgagatag tcgcgcgcgc cttccggcga
    10141 cgaggccgtc aggatcggcg tctgaaactc gaagaagccc gcttcgacca tgcgccggcg
    10201 caaggacgcg ataatctccg cgcgcttcat gacgttgttg tgcaacgtct cccggcgaag
    10261 atcgaggaag cggtgtttga gccgggtctc ttccggatag tccggctcgc cgaacaccgg
    10321 cagcgggagt tcggccgatt ccgacaagac ctcaagcccc tcgacccgca cctcgacctg
    10381 gcccgtgggc aggttggggt tgatggtgtc ctcggagcgc gccaccacct tgccgtcgat
    10441 cttgacgacc cattcgggct tcagggtctc ggccgcggcg aaaaccggcg aatcgggatc
    10501 ggccacgatc tgggtgatgc cgtaatggtc gcgcaggtcg atgaacaata ggccgccatg
    10561 atcgcgaatc cggtgcaccc agccggaaat acggacggac gtgccgacat cgtcgctgcg
    10621 gagcgcgccg caagtgtggg tgcgataagc gtgcatggct ctgaaaggac tcaagggtgg
    10681 gaggtcagct tcggtgccgc ggaacaccgc acaggaagcg cgcccttgtc aaggcgggcg
    10741 cctccagcgc ggcccttcgc ctcggcatgg gacggcaggc gagccagggg cccgcgcgcg
    10801 catcaggcga atatcgcctg ctttcgggga atttccggca ttgaccgcat ctgcggcccc
    10861 cggccctgtc cgaaaaattc ccacattgcc cggttacggg gtgccggacg gtaggctata
    10921 cccgcccccc atggatttga ttacccgtac tgaggacctc gcggcggctt gcggcaagct
    10981 ctccaccgct tccttcgtca ccgtcgatac cgagttcatg cgcgagcaga ccttctggcc
    11041 gcgcctgtgc ctcattcaga tcgccggcgg cgagaccgag atcctgatcg acagcctggc
    11101 gcccgatctc gacctccagc ccttcttcga cctcatggtc gacgagagcg tgctgaaggt
    11161 ctttcactcc gcgcgccagg acatcgagat cgtgcatcac ctggcgggcg tcgtgccaca
    11221 accgatcttc gacacgcaag tcgcggccat ggtgtgcggc ttcggcgagg cggtcagcta
    11281 ttcgatgttg gtgaagcggc tgctggggca caacctcgac aagagctccc gcttcaccga
    11341 ctggagccgc cgccctctca ccgaccggca gctgacctat gcgctcggcg atgtgaccta
    11401 cctccgcgac ctttacccca agctccgcgc gcagctcgac aagtccgagc gggccagctg
    11461 gctcagcgag gagatggggg tgctgaccga ccccgcgacg tacgaggctc accccgagca
    11521 tgcctggcgc cggctcaaga tgcggatcaa gacgccgaag gcgctggcga tcctgatgga
    11581 gctggccgct tggcgcgagc gcgaagcgca gacccaagac gtaccgcgcg gacgcatcct
    11641 caaagacgag gcgctttacg atatcgccgg tcaagccccg cgcacggcgg aggatctcgg
    11701 atccttgcgc tcgctgcaca acggcttcgc ccgctcgcag cgcggccgcg ccgtgttgga
    11761 gacggtgcag aagggcctcg accgtgatcc cagcacagtc ccgccgcttc gacgtggcga
    11821 gcccatgccg ccggaagcgc aggcgatcgt cgatctcttg cgcgtgttgc taaaagcgac
    11881 cgcgggacgc cacggcgtgg cgccgaaact catcgctacg tccgacgatc tggagcagat
    11941 tgcccgttcc gacgatgcgg acgcggccgc tttgcgcggc tggcggcgca agctgttcgg
    12001 ggaagacgcc cttgctctga agcgcggaga cttgtctctc gcaatccgga agggcgcggt
    12061 acgggtgcta ccggcggaag atccagaaga caacgcttag aagcgtggca acgatgagcg
    12121 ccgtcgtgaa cggcaggtag aagtagaaat cccctaagtc cacgacgatg tcgccgggaa
    12181 gccggcccaa gccgtattgc acgctggcca tggttagcaa gatgaccagc aacacataaa
    12241 agacgattat gcccgcccgc atggcttaac cccgccgccg gttctcaatg cgcatgcgca
    12301 ccgtgctgcg catgtcagag ggaagctcgt ccaggacttc aagaagtccg cgcagttccg
    12361 aacgcaatcc gtgaatctga tcgatcaggt taagcagcac cggcacgtcc tcctcgtcaa
    12421 acccaagctc gtcctccagc gtgcagatga gatcggcgcg cgcaatatcc gtttcggaat
    12481 agctccgcgc ggtctcgtcc gccggcttga tgagtccctg actcacccaa atccgcagcc
    12541 gggtcaccgt gaggctgtga acgcgcgcaa cgacatcgtg ctcggtcggc atcacgcttg
    12601 ctccttcagc ttgctccgcg gatcgtatgc atggctcttg cgccaatctt ccgcgaagcg
    12661 cttcaagtcg tcatggatcg tttcaggcat catcacggtc agcttcacca actgatcgcc
    12721 tttgcccttc acgccgcggc ccttgaggcg cagcgtctgg cccgagttgg agcccgcggg
    12781 cactgtcacc gcgacgcgcc cgctaatcgt cggcacctcg accttgccgc cgagcaccgc
    12841 ctcgtcgaag ctgatcggca cctcgaccac gatgtcgctg ccctcacggg tgaacacggg
    12901 gtgaccgcga acgccaatct ccacaagcgc atcgcccgga ggcccctcgc ccatccccgg
    12961 catgcctttg cccttgagcc gcagcacttg cccttcgctg acgccgggcg gaatggtcac
    13021 gtcgagcgtg ccgccatcgg gcagcgtgat ccgcgtctta gtgccgttga cggcatccag
    13081 gaagttgatg tcgagatggt actgcgcgtc ttgcccgcgc atggagaact gcgcgccgcc
    13141 acggcggccg ccgcgcgcgc cagtgaacat atcgccgaac agatcgctga agtcgcccat
    13201 gtcctcgaaa cccgccgagg aacggtagcg cgcgccttcc ggaccgccgg catgttcgcg
    13261 gtagtagcgg gcctcggagc gctcctgtcc gctcgcatcg atctcgccgc gatcgtactg
    13321 cccgcgctta tcgggatcct tcaggatcga ataagcgtgg gagattttct tgaacctgtc
    13381 ttcagccgcc ttgtcccccg gattgagatc cgggtgcagc ttcttggcga gcttgcggta
    13441 agccttggtg atttcaccgg ccttcgcggt cttggacacg ccaagttcgg aatagagatc
    13501 ctctgccatc gacccattct aacggctgga gtgaatgtcg ccggtcaggg cgtggattgt
    13561 catgcccggc ttgaactcaa acttgttgtt actggattcc cgcgttcgcg tgaatgacaa
    13621 acctagcccg ccgccagctg gaacggcgcc ccgaccctga tctcaggctc ccggtccagg
    13681 agattggcga gcgtcttgaa acgccgctcc acccgctcac cctcaaggtt cgcgtagggc
    13741 tccggcaact gccagatgag gcggtttcct tcatagctga gcggcgtatg gggcagaaca
    13801 cccggcatgg ccgccgtgac catatgagca gcgcggaagg ccgcacccag gatgcgggca
    13861 cgcttcagcg cttccttgtc gaccagttcc accaaccgct ggctcaactc gtcgttcttg
    13921 atcaccccct cattgcggta gtaggcggcg agcgccagga aggcgcgccc cggatggtcc
    13981 acgccgacga aatcgccatg ggccaccata ttgagacact gggccccgcg gtaatcggga
    14041 tgcgcccgcc aagtgatatc cgacagcagg cacgccgcct gccgcagccg ggtctgctcc
    14101 ggcgtttcca cgggccccgg catgcggaat aacggctcgg tccaggcact gagctcccaa
    14161 gcgttttgct gcgagcgtga gcgctgcttg gcaaggtagg cacacgcctc gatcaacgga
    14221 tccttggcac gttcctcgtc gctcaacagg ttgtagagca ggccttcgcg gataccgagc
    14281 acggacacca cgatggcgcg cggcttcatc tgcttcatga gccgttcaag caccaaggca
    14341 ccgtagggaa tggtttctcg ccgggcgctg gagatgtcgc gaatgccggc aagcgaactt
    14401 tgcgactggt ggtcgagcag cgaagcgaat ttcagcgcct cgtcggcctc gatgcgatag
    14461 ttgtgcatca cccgcaaagg atagttggtc tgcgtcatgt gcagccgcgc gagcgcccgc
    14521 cacgtaccgc cgatggcata gaaatcgcgc ccccggccct tctccagcca gtccagcttg
    14581 gaaaaggccc tatcgacgat ctcccgcgcc ttcttcatat tgccgccgga ctcatcgatc
    14641 agccgcaagc cgccaagcgg catggtgata gcgtcggaga gtcgcccgcc gcgtacgtcg
    14701 atgagctcga gactgccgcc gccgagatcg ccgacgaaac cgtccgccgc gtagatgccg
    14761 gcgatgatac cgagtgcggc aagctccgct tccatcgtgc ccgacaacac cgtgacctgc
    14821 cggccgagga tgcgttccgc ctgggcgatg aactcgggcc cgttctcggc ctcacgcgcc
    14881 gccgccgtgg caatgacctc gattcgctcg gccttcaact ggtcggccag ggagcggaaa
    14941 cgcctaagcg ccctgagcgc ccgcgacacg ccttcggtat cgagcttgcc cgaactggcg
    15001 atggaccgcc cgagcccgca cagcaccttc tcgttgtaga tgggccccgg ggaacgaatc
    15061 gcgtcgtcgt agacgatgag acgcaccgag ttcgagccga tatcgacaac ggcgactggg
    15121 cctcgatcgt tgcgcgaggc catgcgacta gacgttctcg actggacgcg tgaatcgcgg
    15181 cggcatgttc tccgtcaagg acgtgccgcg gccagacagg ctcggattgg tcatgaagta
    15241 gttctgggcg ctgaagggct cctgccccgg ctccacctcg atgcgctggg aactgccatc
    15301 gggcatcaca cgccagctct ggaggttgtc cttgagattc gccaccatga tttgatcgag
    15361 tatctgctcg tgcaccgttg ggttcttgat cggtgtcatg gcctccaccc tgcgattcag
    15421 gttgcgcggc atcatgtcgg cagaactgat atagaccaca gccttgcgat gcggaagccc
    15481 gtgaccggcg ccgaagcaat agatgcgcga gtgctccagg aagcggccga cgatactctt
    15541 tacgtggatg ttgtcggaga gccccggcac accgggccgc aagcaacaaa ttccgcggac
    15601 cacgaggtcg atctccaccc cggcctggct cgcctcgtag agcccatcga tgatctcggg
    15661 atccacaagg gagttcatct tcatccaaat ggcggcgggc cggccggcct tggcgtggta
    15721 catctcctcg cgaatgtggt cgaggatgcg cgcgcggatg ccgtgcggcg acaccgccat
    15781 gtcctccagc tccgccggct ccgcgtagcc cgtcacgtaa ttgaagatgc gcgacaggtc
    15841 gttgccgacc acccggtcag ccgtgaagaa cgagagatcc gtatagattt tggccgtgac
    15901 ctggtggtag ttgccggtgc ccacatgaca gtaggtggcg agttcgccgc gctcgcggcg
    15961 gacgatcagg ctcagcttgg cgtgggtctt cagctctata aagccgtaga ccacctggac
    16021 gcccgcgctt tccagatcgc gcgcccattt gatgttggct tcctcgtcga accgcgcctt
    16081 cagctcgacc accgccgtga ccgacttgcc ggcctcggcg gcgtccttca gcgcctcaat
    16141 gatcggcgag gtcttgctgg tgcggtaaag cgtccatttg atcgccacaa catccggatc
    16201 ggccgtggcc tgcttcagga actgaagcac cacgtcgaac gactcgtacg ggtgatggac
    16261 gatgatgtcc ttcttggcga tggccgcgaa gcaatcgccg tgatgctcac gaatacgctc
    16321 cgggaaccgg ctgacgaaag gctggaactt gagatcgggc cggtcggaga cgatgagctg
    16381 gttcgtgtcc gcaagaccga tcagcccctt ctgcacgaag ctttcgcgct cggagacttt
    16441 cagctcttgc atgacgaagc gctggagacg cgagggcatc gtggcttcca tctcgagccg
    16501 gatgaccgat ccacgcttcc ggcgcttcag cgcggactcg aacacgcgca ccagatcttc
    16561 ggcctcttcc tgcacctcga tgtcgctgtc gcgcacgacc cggaacgcgc cttggcttct
    16621 cacgcgatag ccggggaaga gcctcgacac gaaaatgccg accaccgtct ccagccgaat
    16681 gaagcggatg gcctccttgc tcccctcctc tgcgggaagg cggatgaacc ggtcgagctg
    16741 cggcgggatg ggaagcagcg cgtgcatggt ccggaacgac ccttcgcgca ccagctccag
    16801 tcccagcgta aaaccgaaat tcggaatgaa cgggaacggg tgagccggat cgacggcgat
    16861 cggcgtcaac accgggaaga tgtggttgag gaaatggtcc tcgagccagg tccgttcctg
    16921 gccggcgagc tgatcggcgc tgagcacatg aattcccgcc tcgccgagaa gcgcctccag
    16981 cgtgacccag gtctcctgct ggttcgcggt caagtcggcg acggactcgt tgatgcggtc
    17041 gagctgctct gtgggcgtca gtccgtcttg gctaggcgtt tcgatgccgg cgacgacctg
    17101 cgcgcgcaag cctgccacgc gcaccatgta aaactcatcg agattgttgg ccgagatcga
    17161 taggaagcgc aggcgctcca atagcggctg ccgctcgttc ccggcctctt ccatcacacg
    17221 cttgttgaaa ttcagccagg acagttcccg gttgtagaag cggtccggcc cgaaggcagc
    17281 aatgcgggca gcagcgccat cgggtccgac ctgttcagaa atgttcatcg atatcctcgt
    17341 gaagacctgc gcctcacccc tcctaggggg ttgttccaag cgcatggtac tctgtcaaat
    17401 gtatcacttg cgttacggaa aacatcaatc cgctgcgtca cttatcgcta acttcaccct
    17461 ctagcgtctc ggtcaccaat tggcggctga tcttgcggcc tgagcggagc gccacccggt
    17521 ccaccgcctc cacggccgcc gcggcggcct ccaaggaacg atcgatccgt accgccatga
    17581 actccagcac cttgggatcg atgtcgagct gccggtcggc gaaatgcttg agcatcacgg
    17641 tccgcagcag cgcttcgtcg ggaacgccga tctccgtgac cggaacggcg ctgagccgcg
    17701 aggcaaggtc gggcaacccg acctcccagc ggtttgcgct gctccgcgac gtcaacagca
    17761 cgaagaggcg tttctcgcgc gccaggttga gcagatggaa caagctcttc tcgtcgtagc
    17821 ccgcgcggtc ggcgtcctcc accgcgacag gcatgctggc gggaagccga tcaatgacct
    17881 cgatgcccag attctccgga tcgagcgcga tggcgccggc gcgtccctgc cacacgcgaa
    17941 cgagatgcgt cttgccgctt gccggcggcc cggtcagaag ctgcacctgg tcctgccatt
    18001 gcggccacgc gtcgaccagc ttgacagccg cggcgttgca atcgctcaca aggaagtcct
    18061 ccgcgccgag cgcggcgcgg tgcggcagat cgaaggcaag ctgagcgagg ctcacgaggc
    18121 tctggcccgc gtccgggggg cgcgaacctt gctttctctg cggcggcgcc ggatggcgcc
    18181 cggttccggc tcgtagctgt tcatatgttg gagccatgcc cagaaatacg cggcggccga
    18241 aaggatggtg aaggtcccgg tcacccagat cagcagccag atgagccagt ccaatccgag
    18301 tccgaggccg agttccgcca gcaccacgcc tgccagcgcg atctgcgcgc aagtgttcac
    18361 tttgctgata agcagcggct tcacggtgat cggccgcgac atcatccagg cgagcagcac
    18421 ggcgccgacg ataagaatgt cccggctcac cacggcgatc accagccaca ccggcaaatg
    18481 gcccgcgagc ccaagcgtga cgaagatcgt cacgagcagc gccttgtccg cgatgggatc
    18541 gagataggcg ccgagttcgg tgtgccagcc atagcgcttg gcaaggaacc cgtcggccgc
    18601 gtcggagagc ccggccagca cgaacacccc aaacgccaca agcatctcat tatcgatgat
    18661 gagccacacc agcagcggca cggccacgat ccgcgcgagc gtcagtgtgt tggggatatt
    18721 caccagcagt tctccaaata atccggccgt cccaaagtgg attttagcag attccgccgg
    18781 tgaattggcc cggcgccgcg cgtgacgaaa gtcagcacca tgtggggatt caatctgcga
    18841 gcagccacgt ctaattgcct gaaacggttg gaatgcgtcg cctgtgaggc ctttccgcct
    18901 tgacggctcc cgtccggcac gattgttatg ccaaatcctt cgaacttgca ccacgagtgg
    18961 ctcaactgcc cccgcaatcc aaaagcccag acccacacgg caagccgctg acctatcgtg
    19021 acgcgggcgt cgatatcgat gccggcaatg cgctggtcga aaagctcggg cccctggccg
    19081 ccaagaccaa gcgggcgggc gtccttggcg gccttggcgg gttcggcggc ctcttcgacc
    19141 tcaaggcttg tggctacaag gacccgcttc tcgtcgccgc caccgacggc gtcggcacca
    19201 agctcaagct cgccatcgag acggggctgc acgcgtggat cggcatcgac cttgtcgcca
    19261 tgagcgtgaa cgatctcgtg gtgcaaggcg ccgagccgct gttcttcctc gactacttct
    19321 cgaccggtgc gctcgatgtc ggcgtcgccg agacgatcgt ggggagcatc gcgcaaggct
    19381 gcgccgaggc cggctgcgcg ctgatcggcg gggagaccgc cgagatgccc ggcctctatg
    19441 agccgggcga ctacgacctg gccggcttcg ccgtgggcgc cgtggaacgg gacagcctct
    19501 tgccgcgcac cgacctcgcc gagggcgatg tgctgatcgg gctcccctcc tctggggttc
    19561 acgccaacgg cttctcgctg gtccgcaagg tcgtcgcggc gagcgggctt gggtgggacg
    19621 atcctgcccc ctttgcgccg agcctgaagc tcggccaagc gctcctcacc ccgacacgga
    19681 tctatgtccg ccagctcctt gatacgattc gcggaaatcc gggggcggtg aaggcgctat
    19741 gccacatcac cggcggcggg ctgatcgaca atctaccgcg cgtcctgcct gcaggtctca
    19801 ccgccgccat tgaccaaagc gccatgcccc tgccgccagt gtttgaatgg ctccaggcgg
    19861 aggcgcggct cgaccccctc gagatgtacc gcaccttcaa ttgcgggatc ggcatggtgg
    19921 ccgtcgcggc cgcggacgat gcgacgcgcg tgcgagacgc cctcggggcg gaagcggtcg
    19981 tgatcggcac ccttgttcag cgcggagacg gcgcagccgt ccggctcgat ggaaagcgga
    20041 gcgcgtgatg acgaagaagc gcgtcggcgt cctcatctcc gggcgcggca gcaaccttca
    20101 ggcgctgatc gaggcggcca aggcgcctga ctacccggcg aagctcgcac tcgtcgtctc
    20161 caacgtagcg ggcgcgcaag gtctcgcccg ggccgacgat gcgggcattc caacgcaggt
    20221 ggtcgaccac aaggccttcg cgagccgccc cggtttcgag gccgtgctgg atgaggctct
    20281 ccacggcgcg ggcgtggatc tgttgtgcaa tgccggcttc atgcggcttc tgacggagga
    20341 cttcgtgacc cgctggctga accgccatct caatatccac ccctccctgc tcccggcctt
    20401 caagggactc aatacccacg cgcgggtcgt ggaggcgggc gccaaaatca ccggctgcac
    20461 ggtccatttt gtgcgcgcgg ccatggatga cggcccgatc gtggcccagg ccgccgtacc
    20521 cgttgcccca gacgataccg aggatagcct ggccgcacgg gtcctggcgg cggagcacaa
    20581 gctctacccc cacgcgctcc ggctggtcgc ctcgggggac gtccgcgtgg agcgggagca
    20641 tgtggtcgga atttcacggc cggagggcga aaatgccctc ttttcgccgc cacttcccgg
    20701 cacgctttga tcctcgcgcc atcggaccta agatggaaga ttggacagcg catgttgacc
    20761 cagctcaaca tgcggcgttc ccaaccatgg acaatccgga cggctggatg ttaggaacgg
    20821 ccaattcaac acaattcccg cgaactgcca taggggaagc ccctagggtc gaggcagcgc
    20881 ggagggccgt ttcctgagct gcgggttggc cctatggggt cgaacccgag attgaacgct
    20941 caggagcggg ggaagggcgt aaatggcgga catgacggtc gcctattctc cgtcacacgg
    21001 gcttggcgaa gagcagagca tctggttcaa gctcacaccg cttttgaacc aggagaaccc
    21061 tatgcaggtt ctcgcggcgc tcagcgacaa atacaacggc gtcatcccga tcaatctaaa
    21121 gaaccagcgc gtcgtgctct tgagcgagcc cgagtacgcc gagcgcgtgc tcgtaacaaa
    21181 cgccgacaac tacacaaagt atttcgatgg gctccgcccc atcttcggcc gcagcatgat
    21241 caccatcgac ggtgcgctgt ggcagaaaat tcgcacacct cagcaggcgg ccttccatcc
    21301 gcagatgttc gaggagtact tcccctatct gatgtcggcc atcgactcca agatggaccg
    21361 ctgggcgaaa ttcgcttcga ccggcgagac catcgagatg gtggaagaga cctggacgct
    21421 cgccgccgaa atgatttgca aggcgctgtt cgaccgcgag atgccgttca acccacactt
    21481 cgtgttcaag caggtgaaga cctacaccga cgtgatgaac cacaagtcca tccgtctgaa
    21541 gaacgtgcgc ggcgaacttg tcgagatgac cggcgaggac acggccaagg ccatggaagt
    21601 gtggcactcg gtgcccgaca tggttatcgg cgccgacgtc gtcgaggatc gcgacaagac
    21661 actgctcaac atgctgctgg aggccgaggc cgatccgcag ttcccggagt tcgaccgccg
    21721 ccaggtcgtc gacgagatca agcaatatct gtgggccggc accgagacca ccgcgctgac
    21781 gctcgcctgg tcactctatc ttctgtcgca gcaccctgaa gccatggagc gcatccgcgc
    21841 cgagacgcgc gccgtctgcg gcgacgccga tccgcaatgg aaccaagtgc agcaactcac
    21901 ctacacgagg caggtgatcc aggagaccat gcgtctcttc ccgccgatct gggcactgat
    21961 ccgcattgcc gccggcgacg acgacatcgc gggccacaag atcagagcgg gcgacaagat
    22021 cgtgatgctg acctatatcg cccatcacaa cccgaagtac tgggacaacc cggagaagtt
    22081 cgatccggag cgtttcgcac cggagcgcgc caagaagcgg gtgaagtact cctacctgcc
    22141 gttcgccgcc ggcaagcggt cttgcattgg cggggcgctg tcgcagatcg aaaacatgct
    22201 ggcgctggtc cagctgctgc ggcgcttcac gcccgaatat gtcgggccca acccggcaaa
    22261 gatccacgcc acggtgacgc tctgcccaaa gggcggcctg cccttccgca tccgcgaact
    22321 gagctagacg cgcttagcgc ttgagtgctt ccgtcaccat ggggcagccg agcgtcttct
    22381 ccagcgcgtc cgtgagttcc tcttccgtct cgacggtcgt gtagagcccg ccggtctcct
    22441 cggcgaggca cctggtctcg acgaccgcct gctcgcctgt ccaggtaatc cccttcaccc
    22501 gcaagccgat gacatgcacg gtgagctgca gcccgtcttt gcggagttcc ttgccgaggt
    22561 cgcatggctt gccgccgcag gtctcttcac cgtcggtgag caccacgatc acgccgggct
    22621 tggcacggta gtcgagcacg cgcgcggctt gggcaaccgc cttcgacaac ggcgtctggc
    22681 ccgcgggcgt caagccgttc accgcatcga gtatgcgctg cgccgcgctt ggcgcagggc
    22741 ttaagtcgag ttgcacgttg catgtctgat aaggaccggg cccataggtg atgagcccga
    22801 cgcggcggaa acgcgtgatg cgcggcaaga tctcggcgag cgcaatgcgc actctgtcga
    22861 tgcggctgac agcggtgggg ttctcgctgc cgtagcccca gccattgccc gacatcgagc
    22921 ccgaagcgtc gaacaccagc atggcgtctt cggtgcaagg cgcagccggc tcgtcggctg
    22981 cgtgcgcgag cctctcgccc accgggctca cggccgcaac aaggacggca aacacaaagc
    23041 ttttgctcag aagacccatg atggtgcaag tttagcccaa cggcgcacgc cagggccgta
    23101 acataccgct agtcgatcgt atgggttctc gggtccggct cgtccgtcgc agcagcgcct
    23161 ggcccacgtt tcgcgggcgg cttgtcgtcg aggtcaggga agcctgcgcg caaataggcc
    23221 acgatctgga agatctgttc gtgggagagc tggtccttga accccggcat ggcggtgccg
    23281 aacggcgccc cgccttcggc gatgctccat aagagatact gatccacggc aaccggctgc
    23341 tgcaccatat aggcgaggag cgccggcgac ggcttcagcg cgtaagcaag atcgccattg
    23401 ccgagccccc attcgccatg acacttctgg caatggctcg catagagctg tccgccttca
    23461 gagatggcgc gcaccgtgta gcccaccgtg ttctccgcct tctgatagtc gtccggtatg
    23521 ccgccattga cgaaggtcga gaagcgcagc atgcgctgcc gctggtcggg atcgagattg
    23581 cgcatgcccc aatcgagggc gggcgactgc gtcttgggga tgtctttggg cacgccgttg
    23641 gcatccggcg gcacatcttc cgcgcgcgag gtgatagccg gcagagcaag cagacccgcc
    23701 aggacagaga ccaaaaggag catacgaggc atggtggcat tcttagccac cacggcggat
    23761 catgccttgc cgtagatcaa aaggaggcga acagccctac ccgatgatct cggccttctt
    23821 aaagcagaga tcgatctcgc gctgggcgct ttccgggctg tcggagccat gcaccgagtt
    23881 ccgctgcatg ttgacgccga aatccttgcg gatcgtgccc ggctccgcat cgaccgggtt
    23941 ggtccgcccc atcaccgagc gatttcgggc gatggcgtcc tccccttcga gcaccatgag
    24001 cacgacaggc cccgaggtca tgaactggat gaggtcctta tagaaggagc ggtccttatg
    24061 gatctcgtag aacttctgag cgtccttctt cttccaccag acccgctttt gcgcgatcac
    24121 gcgcaagccg gccccctcca gacgcgcgat gatcagcccg gtgatgttgc gctcagtggc
    24181 gtcgggctta atgatcgaca tcgtgcgttc gctggccatg cccaagtcct tcttgtcctt
    24241 tgattctttg ccgggcttat agccatcagg aatgacggcc acaaggcgcc taaggtaaac
    24301 gggggcaaaa acctgttggg gggtgccaag caagggcggc ccagaacgcc caaggaggaa
    24361 gaccgatgct gtatgccacg atcaaggtcc tgctgacgag cgtcctcgtg gtcgcagtct
    24421 ccgaggccgc caagcgcagc gccctgttcg gggccattat cgcctcgatc cccctgacct
    24481 ccgttctggc catgatctgg ctctatatcg ataccggcga tacggagaag gtggcccgcc
    24541 tggcctcagg catcttctgg ctggtcctgc cctctctggt cctgtttctg accttgccgc
    24601 tgctgctgcg agccggggtc gatttctatg cgagcctggc cgcctcggtc acgctgacgg
    24661 tcggcgccta tttcgccatg atctatggct tgcggcttgc cggcatcgag atttaggcct
    24721 tctgctgcca gcggccctcc ccatcttgct gccaatagct cacggcgaag cccttgtcct
    24781 tcgcctccgt ccatgcggcc cgcgcctggg acacggcgtc gtcgtcatga ccgtcgaaca
    24841 catagaccac ccgggcgtaa ggcgatgcat cgtcgagcgt ggcgccatcg acgaggaagc
    24901 gcaccgccgc gtcgttggga ttgtccgcct cgacggtcag aaagatcggc tgcatcgccg
    24961 ccgtgccgtc gctggacgtc ccgtggggta ggaagctgtc ctcgcgaaag gtccacagca
    25021 acgtgttcaa cgcctcgacg cgctccgggc tcgaagcttg caccactgcg cgccacccgc
    25081 gttcgagcga gcgttccagc agcgtcggca gcacctgctc cagcgtgcgg ccctcaaggt
    25141 gatagaagta gacttccgtc tcgccgctca tgccttagcg tcccttcaca taagggttct
    25201 cgcctttacg caaattgagc ctgatcggca caccgggcaa gtcaaacgtc tcgcgcaagc
    25261 cgttcacaag atagcgcaca taggcatccg gcagagcctt gggcagcgag cagaacacca
    25321 cgaaggtcgg cggccgcgca ttggcttgcg tcatgtagcg cagcttgatg cggcgccccg
    25381 agacggctgg cggcggatgc gcttccgttg cgccgcgcag ccactggttc agcgtatgcg
    25441 tcggcaaacg ccggttccac atttcgtcgg cggcgaccac cgcccgcatc agcctgccga
    25501 gacccttacc gctgttcgcg gagagcgtga ccagagacac gcctttgact tgcggcaaca
    25561 gccgctggca catctcgcgc aactcggcca gacgttgctg ccggtcttcc acgaggtccc
    25621 atttgttgat ggcgatgacg agcgcccgtc cttcttgtgc aatcaagtcg gcaatctgga
    25681 ggtcctgctt ctcgaacggt tgttccgcat cgagcagcaa gatcaccacc tcggcgaagc
    25741 ggatagcttt gagcgtatcg ccgacggaga gcacctccgc cttcttctcg atacgcgcct
    25801 tgcgccgcag ccctgcggtg tcgaacacgc gcatgggccg gccgttccac tcaatctccg
    25861 acgcaatcgc atcacgggtg atgcccgctt ccggacccgt aatcatgcgc tcctcgccaa
    25921 gcagcgcgtt gacaagcgtg gacttgccca cattgggacg tccgacgacg gcgatgcgca
    25981 acggatgcgg acctgagtcg gcctccacgg catccgtatc gggctccggc ttgcgagcgc
    26041 tgcaagcctg ctcgatggcc tccgcaattt cgccgatgcc aagatcgtgc tcggcggaga
    26101 tcgccaaagg ctcgcccagt cccaacgcaa aggcttcgta gtagcccgcc tccgcggccc
    26161 ggccctccga cttgttggcc gccagcacca cgggctttcc cgacgcgcgg acgagttcgc
    26221 cgaagatttc gtccgccgct gtgatcccga cacgtgcatc gatgagaaac agaacgagat
    26281 ccgcttgcgc gatggcttcg tccgtctgct ggcgcatgcg cgcgctgagg ccctcatcgc
    26341 ccgactcgag ccccgccgta tcgaccagcg tgacggcaca ggcccctaag accgtctcgg
    26401 cctcgcgccg gtcccgggtc aggcccggct ggtcgtcgac cagcgccagc cgcttaccgg
    26461 cgagccggtt gaacaaggtg gacttgccca cattgggccg tccgacaatt gcgacagtga
    26521 aggacatggg gaccgttacg gcgcgagagg aaaccgggcg gcgcgagagg ccagcccaag
    26581 cgtcaattaa gagcaatcag gtcggcgtcg tccgacagga tatacatccg gccgcccgcc
    26641 acaacgggcg tgacgaacac atccgtatcg aggtcgatct gcgttccaag ctgtccggtc
    26701 cgcgcatcgg ccccgaccaa gagcccgtcc ccggagacga cccaaacccg gctgcctgca
    26761 agcaccggac cgctccaacg cgaactcgcc ggcaaatcgc taacccagcg gatttggccg
    26821 tccctgcggc cgatcgccat gaggcgtccc cgcgtatcca ccacgaagac aacatcgccc
    26881 gcgagccaag gcatctgcgt gctggcgata ttgcgcgtcc acacgcgcgc gccggagccc
    26941 gccgaggtcg caatcatcct tccgccgtga ctgaccgcgt aaaccacacc gcggtcgatc
    27001 accggccggg caggatcgcc gagaatgccg gcggccgacg tgtccgacga ccggctgagc
    27061 gagtcgctcc aggctcgttt gccgctgcca gtctggaagg ccatgatgtc gcctgccggg
    27121 aacggcgcca ccaccgtccc gctcgcgacg gcggggcttg cgttgctgag caacgtcgtg
    27181 ggctgcggca gtccacgcgc cctccacagc ttgctgccag tgctggcgtt caacgcatgc
    27241 agcgtgttct cggtggatac gaaaaagact ttaccgcccg cgaccgtcgg cgaattgcgg
    27301 atcggctcac caatcggtgt ggtccacagg acagcgccgc tgctcgcgtt caaggcgaca
    27361 accgtcccgt agcccgtcgt cacataaatt gtgttgctct cgaatgcgag gccgccgcca
    27421 aagccctctt tgctgctctc gttatctggc gtgacgctaa cgccccaggc cttggcgccg
    27481 ctcgaggcag agaacgcaga gacatttccc gcagcatcaa gtgtgtagac ccggccgccg
    27541 gcgacaatcg gcaccgcgct caaacgcccc ctcgacgacg acccggtgcc ggcgtcgacg
    27601 gaccaggctt tgcgcaagcc gccggcaagc gccaagtgac cgagattgtt cgaggccgac
    27661 ccgccaggct gcgcccaact cgcattagac cgcgccggcg gcagcgccac ggcccgcggt
    27721 gagatttggg ccgcattgaa ctggcctggg tcggtaatga ccgacacccg ctcgccgggc
    27781 agacgctctt cttcttctcc gaaaggattc ttgatggccg acaggctgcc acaagccgtg
    27841 aggacgatcg cgagcgagac ggtcagtccc gcgccgagaa ggcgatagcc catgtctcgt
    27901 tcacttctcg ggcccacgtt tcgagccatc ccctgatcga acgcgccctg ttggacgagc
    27961 tagtttgtcg tgctgctgct ttgcgccggc gtactgctca cgatcaaggc tagcatcatg
    28021 ttggcacgct cccgcaaatt cggcggcgtg ttctgatcgg caaggagcgc gctgaattgc
    28081 tcctcggcgg cacgcatgtc cttgtgctgg taggctgaca gacccagtag ctcccgcgcc
    28141 gagaagcgcc agcgcgaatt gttctcgacc agcccattca gccgccgctc catctccgca
    28201 tagtccgcgg catcgacccg cagcgtcgcg gcttggaccg tggcaagacc ttgcagaatg
    28261 ggatcgacgc cggcatccgt ggcaagcgca tcgtaggcgg cgacggcttt gtccgtgtcg
    28321 ccggccttgg cgtcggtcgc cgccagctgg aagcgggaca gcacctggta gccgctgggg
    28381 ccgtccttcg cgagcgtctc gaatgcctca cgcgctttgt cgagatcggc gcctgtcttc
    28441 agcgccagag cttgggtgta ctcggtcccc gcctccttgg cctggcgctc cgtccaatag
    28501 gtccagccct tgtacccggc aacaccagcg atcacggccg cagccaggat aatgatgtaa
    28561 accccgtact ggtcccacag cgccttgtac cgctcgtggc ggacggcttc gtcgacctcg
    28621 cggaaaaaac tattgtcgct cataaggctg cggactcttt cggtgacaaa ggacggtgcg
    28681 atcgaggccg actgtggccc ccaacctcac gccgtatagt gaaggccggt tgctcacgca
    28741 aggctcaagc ctccgcgcga tagcgacaaa cgtggctctt ggttagccct cgaaggctaa
    28801 ctgcctgtca tttcagtctt tttcgtgtct tcgtcgtcgc cgccgtcctc gtcgatcatc
    28861 ggataggtat gggcatccga tgggaaggcg cgccgacgga catcggcgga atacgctttc
    28921 acggcgccct cgatggcgtc cccgagctgg gcatattcct tcacgaattt cggcacgcgg
    28981 ggcgagagcc ccagcatgtc ctcaagcacg aggatctgcc cgtcacactc ggccgaggcg
    29041 ccgatgccga cggtcgggat agaaatttgc ttggtgatgc gcgcggcgag gagctcggcc
    29101 aaggcctcga gcaccaccgc aaaggccccg gccccggcca ctgccttggc gtcctcctcg
    29161 aagggcgccc actccgaacg gtggcggcct tgtggcttga agctgccgat ctggttcacc
    29221 gcttggggcg tcaacccgat atggcccatc accggaatac cccggctcgt cagataatcg
    29281 atggtttcgg ccatgtgcac gccgccttcg atcttgacgg caccagcacc ggtttccttc
    29341 atgatgcgcg cggcgttgcg gaacgcgacc gccgggctct cctcgtaaga cccgaaaggc
    29401 atatcgacca cgacgagcgc gtgcttggag ccgcgcacca ccgcttgagc atgcatgatc
    29461 atgagatcga gcggcacggg aaccgtggtc tcgtagccat gcatcaccat gccgagcgaa
    29521 tcgcccacca gcagcaagtc cacatgcgga tcgatgatct gcgccgtatg ggcgtggtag
    29581 gcggtcagcg ccacgatagg cgccccgccc ttgcggccgg cgatttgcgg tgcggtgacc
    29641 cgcttgacgt caaagctctg cgacacggac ttcccctggg tcaatggcga tttcgggcta
    29701 actttatgac agatatatag tgatttgtgc actgcaaaaa agtcctacga cgtgtggact
    29761 tagtggggca caaccgcccg gccgatcagg agtggatgca gccagatcag gaatatcacg
    29821 tagaacacgc cccccaggac gacagctatc acgtcgttgc gggcgccgcc tggcgccgcg
    29881 gacacgatga gttccctggg ctgccgccgt ttcaaggaaa tgcggtcgaa gatggcatac
    29941 gcaagaaacg acccgaacag gatgatggag gcgagatcgc cattggcgat cagatgcgcc
    30001 agcgcccacg tcttgatggc aacgagatag gggtgcttga gcttcgcttt gatcccaccc
    30061 gggatatagg ccgcgaacag gaagatgaag gccggcagca tgagcgcgat cgccacccag
    30121 cgcgtccagg ccggcggcgg ccagacttgc acgaacggcg cgacggcaaa gccccagacg
    30181 agaagcgcga agcccgcgat cgacgcgagc gcaaaggcga cctggtagcc tttctcgccg
    30241 agccgcccga tcagcgcctc gcgaacatgc ggaaatgccg gcaccagatg gatgccgaga
    30301 aacacaacga tgccgatgat gagtagcgtc atgacgcagg tcccagcttt tttgattcag
    30361 ccctttgttg ggcggcacca aagctcaagg gtggggaagc gtcaaccgga agcgcggtgc
    30421 tgtccggcgc tagtctgctg ccgatccacg tccggtggcc aggaacgcgc agccttcgcc
    30481 tttcggagac tcgcttctct gtcacggcgg ctttcggcgc gatcagcggc gcttgggcgg
    30541 cgtggccctg gccgaatcgg cgcccgccag cgcataggcc ttctcggtgg tcacgttaag
    30601 cgcgtcctcc acctgttgcc ggtatggctc cagctcgcga acgttcaggt ccggcggcac
    30661 ctggataggg tcgcccatgg cgatgccgag tttcgagaac ggcagattga acgtcatgcg
    30721 gtcccatgaa ttcaccgcgg cgtagcgcga gctcgccaca gcgaacggca cgaccggccg
    30781 gcccgacatg ctggcgatcg tgacaatgcc gcgtccgcat ttgcgggctg ggcccggcgg
    30841 cacgtcggcg gtcatggcga tgctgtagcc ctcccgtaaa gaggcgaccg ccccgtgggc
    30901 ggcctcggca ccgccgcgat cgcggcggcg ctctcccgca cccgccccgc gaatgagttc
    30961 cagaccgaat ccacgcaaga cgcgggcgag cgcctcggcg tcctcatggc gtgacaccat
    31021 tgcccgtccc ggaacttcct gcggatagat ccccggcagc agaaagaact gcccatgcca
    31081 cagcgcgatg atctgaggat ggttctccca ggcgcgcgcc acatagtccg gcggatcgga
    31141 aacgcgcgag gacgtgccga gcacgagctt gacgtagttg gagaagacgg gagccgcgag
    31201 aatttgcgca gggcggcgca gacgcacctt ttgccgccgg atccatgaat tgcccatggg
    31261 tagctcgatc ccccagtctt ccagtctcaa gattcggcgc gagaataacg aagtcggacg
    31321 tcacttcttg tagctgacac agcaccaata tggggtgcgc tcggaaaaca caatgttttc
    31381 gaaaccggcc ttg
//
LOCUS       DBNHVU010000045        10940 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_536211, whole genome shotgun sequence.
ACCESSION   DBNHVU010000045 DBNHVU010000000
VERSION     DBNHVU010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 10940)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10940)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10940
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_536211"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="AC16Y7_01705"
     CDS             <1..469
                     /locus_tag="AC16Y7_01705"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR5708124.1"
                     /translation="WGIIDGKDVETPVYHQTHSCYAVAAFHEALRILLSYGRAAKAAD
                     YRYHEAALRQAVEAMGCEVTSDMPSLVVLNLPGKLAGREKELVQGARSQGFGIWPTLS
                     EPVQVRIGILNQLSPEQITEIVSRFADAMLAMGAEVDKAAVLNQLKSYYAKAA"
     gene            complement(542..1156)
                     /locus_tag="AC16Y7_01710"
     CDS             complement(542..1156)
                     /locus_tag="AC16Y7_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HIR5708125.1"
                     /translation="MAQRKSSTRKSRSRKTTGRRRTRKRSTKNTLAKQLGKVIALVLV
                     LGLVLLLQRYCGIAPVPFGPNANIVSIDGDTLRAGDGTEYRIFGIDAPELKQTCWELG
                     GKQWQCGRAAKVALTKLIKGGDVRCEVKNTDRYGRSVAKCSAKGVPDVGEALVRDGYA
                     IDLGRKTRHAYASVEAEARAANRGIWRGTFQRPSEWRYENPRRN"
     gene            complement(1156..1962)
                     /locus_tag="AC16Y7_01715"
     CDS             complement(1156..1962)
                     /locus_tag="AC16Y7_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008122082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HIR5708126.1"
                     /translation="MAHKSLMALASAMLLFFASTAEAARCGKSAAGFEKWKRAFAKEA
                     RANGIGQRGLNALKGTRYAYGTIKADRGQHSFKLSLNQFMKKRGASAIASQGKRLKRS
                     NAKLLAAIERRYGVPAGPLLAIWGMETGFGRYMGRQNTLSAVATLAYDCRRPEFFTEH
                     LYAALQLIDQRRLAAGQRGAMHGEVGHTQFLPKSVLLYAVDGDGNGRADLNSKADALA
                     STANFLRGHGWQPGAGYQPGQPNFRAIQGWNAAGVYQQTIAIVGRQIDGG"
     gene            2189..3058
                     /locus_tag="AC16Y7_01720"
     CDS             2189..3058
                     /locus_tag="AC16Y7_01720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR5708127.1"
                     /translation="MAVAALSTVLTGFVVAGGAIAVPYVMRSPPVQDYSLKALEPAAH
                     RTLVLHGNDGQPFASRGGCVAELVSLGEVPPHVVDALLSMEDRRFYYHFGVDPIGLAR
                     AALANRAAGRIVQGGSTLTQQLVKYSLLSTDRTLERKQKEAWLALSLELRLTKDEILE
                     RYLSSAYFGQGCYGLRAAAKRYFEVPVADLSLPQAAYLVGLLKAPTALNRDPEAAKAR
                     SEAVFDAMLDNGKLDPEQRTALVHVLPRIESRETVGSYYADWVASTAGSAQRQLCSVA
                     GPHVLRSGASASC"
     gene            3066..4100
                     /locus_tag="AC16Y7_01725"
     CDS             3066..4100
                     /locus_tag="AC16Y7_01725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR5708128.1"
                     /translation="MGRVLAKQGKARRATQAAMVVMRSDGRVLAMVGGRDYDQSKFNR
                     AVQARRQPGSSFKLFVYLAGLRAGLKPSSIVVDQPVRVGRYTPRNFGNRYLGTIRLHR
                     AFSSFVNTVAVQISEATRRKPVIQAARDLGITTPLKARPSLALGAFEVTLLEMTSAYA
                     AVAAGAYPVKPWSVTGYEEIDLEVTPPKGAGRWRLNERQYMRALLRAVVKNGSGRRAK
                     LPVNSFGKTGTSQRYRDAWFIGFAGNLVVGAWVGNDNSKPMKGVTGGSLPAEIWGRFM
                     RKAIEHDGFRNNGRVAAFDAKPRKRKVRFVKAGFWLDAGKANNKKKKKQKKKKKKNKK
                     RKKNKKAKKR"
     gene            4172..4384
                     /locus_tag="AC16Y7_01730"
     CDS             4172..4384
                     /locus_tag="AC16Y7_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708129.1"
                     /translation="MIPVMADRSRLATERMSVSIVRAAPRIGRQRVTFCALEGSLLIL
                     IEADSEDEAHAICAEQGLEFHSLCEG"
     gene            complement(4409..4795)
                     /locus_tag="AC16Y7_01735"
     CDS             complement(4409..4795)
                     /locus_tag="AC16Y7_01735"
                     /inference="COORDINATES: protein motif:HMM:NF020918.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HIR5708130.1"
                     /translation="MPDNKMFEIPTEMRELAEKNVEEARVAYGQFMEFMSQAMNAWSS
                     TGAPEAGNFKALQERAVSFAKENAERSFSLASDLANAKDMQDVVTLQSRYVQTQMQTF
                     GIQAQQLSWLMADAFRNMQSKPPKKD"
     gene            4981..6693
                     /locus_tag="AC16Y7_01740"
     CDS             4981..6693
                     /locus_tag="AC16Y7_01740"
                     /inference="COORDINATES: protein motif:HMM:NF015148.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HIR5708131.1"
                     /translation="MTPQTDSPAPGPAELTPRALATGLLLGALLAPCNVYSGLKIGWS
                     FNMSITALLLAFAFWMPLARLCKLAPWGMLESNINQTTASAAAAITSSGLVAPIPALA
                     VMTGENLHWAPLTMWVFTVSFLGVWVGWYLRPRMIVQSNLVFPAGMATAQTMLDMFAH
                     RREAMARVKVLLSALIGAVLFNVVDKLVWHIPRLGPSVTASKLTFVFDPSLLLIGFGG
                     IIGLRAGLGLLFGALIAWGLIAPQLIAHDIVIVSQDASNWFEPLVGWLLWPGVAMMLA
                     GSLTSFGASLLDVSKPHRRPQARAQTSGSGRLQFAGFVAAAALAIALQIILFDVHWTM
                     ALASIAIAFALATVAARVVGETSVAPIGAIGKLSQLSFGVMAPGQPITNLMTANVAGG
                     AAGQSADLLNDFRAGYEVGAHPARQVAAQCAGVVVGSLVSSLVYLMLIPDPTTQLFTP
                     EWPAPAVAVWKAVAEALSHGLNGIAPSALIAMAIGAVLGIGLALAEHWLDKKYLLFVP
                     SGATLGLAFVIPASTSLMLFAGAALASLAHKFAPNWSARFLLSVAAGLVAGESLFGVV
                     SVWF"
     gene            6752..7825
                     /locus_tag="AC16Y7_01745"
     CDS             6752..7825
                     /locus_tag="AC16Y7_01745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015457.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5708132.1"
                     /translation="MTTGMIGGGFYNRHSAPQANALEYVLPWLIEAAAAMDLSPVPEA
                     VTLADFGCSEGQNSIKVMHAVLPVLRSRTSRPLVTIHSDLPTNDFSEALLALRPQGQS
                     VFGENTYSAILGGSMFDRLLPPRSLHVAMTFNAVGFLSRRPLDRLPGYILPNGPSALR
                     NVGAVTETERSAFAEQAGADLESFLRARAVELVPGGKLLVQVFGMGEEYRTCDGLYDV
                     LNDAVLEAVTSGQIDRATYDAYYQPVYFRTLKELLTPLEAGTGVCTDLFSIDRAEFYE
                     VPVPFVEDFKQNGAAAAYASAYTRFLRAFTEPVLRLALGDNPKTDAVVGDVFGRVEQL
                     LTAHPERYRFDYVSVAALMTRTG"
     gene            complement(7839..8801)
                     /gene="trxB"
                     /locus_tag="AC16Y7_01750"
     CDS             complement(7839..8801)
                     /gene="trxB"
                     /locus_tag="AC16Y7_01750"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008547726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR5708133.1"
                     /translation="MGSRHAKVIIIGAGPAGYTAAIYAARAMLEPVLIEGIQPGGQMT
                     ITTDVENYPGFADVIQGPWLMDQMRAQAEHVGTDLVADTIVEADLKQRPFKLKGDSGT
                     EYTADAVIIATGAKARWLGLPSEDKFQGFGVSACATCDGFFYRGKNVIVVGGGNTAVE
                     EALYLANLADKVTLVHRRDALRAEKILQERLFKHPKIEVLWDHQLAEVLGDEDPLGVT
                     AAKLRNAKTGDETTMPIDGVFIAIGHSPQTELFKDQLALKQGGYIKTEPGSTATSLEG
                     VYAAGDVTDDVYRQAVTAAGMGCMAALDVERYLAAMEAHAEAAE"
     gene            complement(8885..9952)
                     /locus_tag="AC16Y7_01755"
     CDS             complement(8885..9952)
                     /locus_tag="AC16Y7_01755"
                     /inference="COORDINATES: protein motif:HMM:NF018017.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HIR5708134.1"
                     /translation="MNVPHSGNFGDPQSTPGGDAEGAAALIAESRKARTAKTWIVTDG
                     SVGMEAQGIAMAEAVGLPYALKRVAPKGLLRLLPAPGQLLLPPQALLRQTKSNEALEP
                     PWPRLIISIGRRGVPMALAIKRLSGAYALHIQNPKVPARLFDLVAAPAHDNFEGPNVI
                     TTFGAVHGVTAERLAEAGERFAPTIAKLPHPRVAVELGGESQAFSFPPETAAAFGAKL
                     ADMARETGGSLLVTPSRRTRVESLDALSKAIADTPHFVWDETGYNPYFAFLALADAIV
                     VTEDSVNMVTEAAGTGKPVYVQAMPGRSTRLARFHALMRERGATRPFEGHLESWTYAP
                     INDTEMVAARIREALDLETKA"
     gene            10080..10577
                     /locus_tag="AC16Y7_01760"
     CDS             10080..10577
                     /locus_tag="AC16Y7_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR5708135.1"
                     /translation="MRAARLDATDWLILRELQADGRITNIELARKVGISAPPCLRRVR
                     ALEEAGIIEGFTAILNDKALGFDVSAFAMIKLHSQAEADLIAFEERVAACAIVRDCYM
                     LSGEIDFLLRCVAPDLSAFQEFIIHELTAAPNVESVKTTLVIRTSKHAAGVPTELVSG
                     EMRAK"
     gene            complement(10581..>10940)
                     /locus_tag="AC16Y7_01765"
     CDS             complement(10581..>10940)
                     /locus_tag="AC16Y7_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015687422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03808 family TAT-translocated repetitive
                     protein"
                     /protein_id="HIR5708136.1"
                     /translation="NLFLRKQGEVRGVGIGVEADTVVTGNVIEGAPAYGIFAGWAKYL
                     RDVSVTDNVIRKSHVGIAVSVDPSAGPALITDNLIDGAKDGAIRAMKGRTPVGPDLAH
                     ESAEAYRNLAVYANVAR"
BASE COUNT         1830 a         3402 c         3575 g         2133 t
ORIGIN      
        1 ttggggcatc atcgacggca aggatgtcga aacgccggtc tatcaccaga cccattcctg
       61 ctacgccgtg gcggcgtttc acgaggcgct gcgcatcctg ctgtcctatg gccgtgccgc
      121 caaggcggcg gactaccggt atcacgaagc cgcgttgcgc caggccgtcg aggccatggg
      181 ctgcgaggtg acgtcggata tgccgagcct cgtggtgctc aacctgccgg gcaagcttgc
      241 cggccgggag aaggaattgg tgcagggcgc acggtcccag ggctttggca tttggccgac
      301 cctgtcggag cccgtgcagg tccgtatcgg tatcctcaac cagctcagcc ctgagcagat
      361 caccgagatc gtcagccgct tcgccgacgc catgctggcc atgggcgcgg aggtcgacaa
      421 ggctgcggtg ctgaaccagt tgaagtccta ctacgccaaa gccgcgtagg cagacaggat
      481 cacgcgcgac cggcgtctgc ccaagggctc ctcgccggtc gcgcaaactc ttcttttcaa
      541 atcagttcct gcgcgggttt tcgtagcgcc actcggacgg ccgctggaag gtaccgcgcc
      601 agatgccgcg attggcggcg cgggcctcgg cctctacgct cgcataagcg tgccgggtct
      661 tgcggcccag atcgatggca tagccgtcgc ggaccagtgc ttctccgaca tcgggcacgc
      721 ccttggcgct gcatttggcg acggagcggc catagcggtc cgtgttcttc acctcgcacc
      781 tcacgtcgcc gcctttgatc agcttggtca gcgccacctt cgccgcgcgg ccgcactgcc
      841 attgcttgcc gccgagctcc cagcaggttt gctttagctc gggcgcatca atgccgaaga
      901 tgcggtactc cgtaccgtcg ccggcgcgca gcgtgtcgcc atcgatggat acgatgttgg
      961 cgttcgggcc gaagggcacc ggcgcaatgc cgcaatagcg ttgcagcagc agcacgagcc
     1021 ccagtacgag cacgagcgcg atgaccttgc cgagttgctt ggcgagagtg ttcttggtcg
     1081 accgcttacg tgtacgccgc cgccccgtcg tcttgcgcga tcggctcttg cgcgttgaag
     1141 acttccgttg cgccattagc cgccgtcgat ctggcggccg acaatggcga tggtctgttg
     1201 atagacgccc gccgcattcc agccctggat cgccctgaag ttgggctggc ccggctgata
     1261 gccggcgcct ggttgccagc catggccgcg caagaagttc gcagtggagg cgagggcgtc
     1321 ggccttgctg ttgagatcgg cacgcccatt cccatcgccg tcgaccgcgt agagtaggac
     1381 gctcttcggc agaaactggg tgtgacccac ttcgccatgc atggcgccgc gttgcccggc
     1441 ggccagccgg cgctggtcaa tcagttgcag ggccgcgtag aggtgttcgg tgaagaattc
     1501 gggccgccgg cagtcgtaag cgagcgttgc gaccgcagac agggtgttct gtcgtcccat
     1561 atagcggccg aagccggttt ccatacccca gatggcgagg agcgggcccg ccggaacgcc
     1621 atatcgacgc tcgatggcgg cgaggagctt cgcattggag cgcttcagcc gcttgccctg
     1681 agaggcaatg gccgacgcac cgcgcttttt catgaactga ttcagagaga gtttgaaact
     1741 gtgctggccg cggtccgctt tgatcgtccc gtaggcgtat cgcgtgccct tcaacgcgtt
     1801 gagcccgcgc tggccgatac cgttggcccg cgcctccttg gcaaaggctc gcttccactt
     1861 ttcgaatccg gcggcactct tgccgcatcg cgcggcctca gcagtggagg cgaaaaagag
     1921 gagcatggcg cttgccagcg ccatcagtga tttatgcgcc atttgggcct tccgtgtttc
     1981 agggaggccc gggccgaaag cgccgaaccg ccatccttga gagtcctccc ccaacaagcc
     2041 atttctaccg tgatgtttcc ggccatggca atccgcttag gccgccagag cggggtcgtc
     2101 attgcaccgc cacaaaagta ttgtctcgcg tcggggcagg cgtggaggcg agatgcgcat
     2161 accggggata gtggcgaaga gcgggtactt ggccgttgca gccttgtcga ccgtcttaac
     2221 gggcttcgtc gtcgctggcg gggcaatcgc cgttccgtat gtgatgcgtt caccgccggt
     2281 ccaagactat tctctgaagg cccttgagcc ggcagcgcat cgcaccctgg tccttcacgg
     2341 aaacgatggt cagccgttcg cgagccgggg cggctgcgtg gccgagctgg tgtctcttgg
     2401 ggaggtgccg ccgcacgtcg tcgacgcctt gctctccatg gaagaccgcc gcttctacta
     2461 ccatttcggg gtcgatccga tcgggcttgc gcgcgcggcc ctggccaacc gtgcggccgg
     2521 gcggattgtc caaggcggca gtaccctgac gcagcaattg gtgaagtact cgctgctgtc
     2581 gaccgaccgt acgctggaac ggaagcagaa ggaggcgtgg ctcgcgctgt cgctagagct
     2641 gcgtctcacc aaagatgaga tcctggaacg ctatctgagc agcgcatatt tcggccaagg
     2701 ctgctatggc ctgcgcgcgg cggcgaagcg gtatttcgaa gtgcctgttg cggacctgtc
     2761 gctccctcaa gctgcttacc tggtggggtt actcaaggcg ccgacagcgc tcaaccgcga
     2821 tcccgaggcg gccaaggcac gctccgaagc ggtcttcgac gccatgctcg acaacggcaa
     2881 gctcgacccc gagcagcgca cggcacttgt ccacgtgtta ccgcggatcg agtctcgtga
     2941 gaccgtcggc agctattacg cagactgggt ggccagcacg gcaggttccg cgcaacggca
     3001 actatgctcc gttgccggtc cacacgtcct tcgatccgga gcttcagcat cttgctgaag
     3061 cggcagtggg tagggtcctt gcgaagcagg gtaaggcgcg ccgcgcaacg caagccgcca
     3121 tggtcgtaat gcgctcggac ggacgcgtgc tcgccatggt tggcggccgc gactatgacc
     3181 agagcaagtt caatcgcgca gtacaggcgc gccgccagcc tggctcctcg ttcaaactgt
     3241 tcgtctacct cgccggcttg cgagcggggc tcaagcctag cagcatcgtc gtcgaccagc
     3301 cggtgcgcgt tgggcgctat acgcctcgga acttcggcaa tcgttatctc ggcacgataa
     3361 ggctgcaccg cgcattctcc tcgttcgtca acaccgtggc ggtgcagatc tcagaagcga
     3421 cgcggcgcaa gcccgtgatt caggccgcgc gcgaccttgg catcaccaca ccactcaaag
     3481 cgcgtccgag cctggcgctg ggtgccttcg aggtgacctt gcttgagatg acaagcgcct
     3541 atgctgccgt ggccgccggc gcctatccgg tgaagccttg gtcggtcacc ggctacgagg
     3601 agatcgacct ggaggtcacg ccgcctaagg gcgcgggccg ctggcggctt aacgagcggc
     3661 aatatatgcg cgcgctgctg cgcgccgtgg tcaagaacgg ctcaggccgg agggcaaagc
     3721 tccccgtcaa ttcgttcggc aagaccggca ccagccaaag atatcgcgat gcctggttca
     3781 ttggctttgc cggcaatctc gtcgtgggcg cgtgggtcgg aaacgacaac tccaaaccca
     3841 tgaagggagt cacggggggc agtctgccgg cggagatctg gggcaggttc atgcgcaagg
     3901 cgatcgagca tgatggcttc agaaacaacg gacgtgtggc cgcctttgac gccaagcctc
     3961 gcaagcgtaa agtgaggttc gtcaaggccg gcttctggct ggacgccggc aaggccaaca
     4021 acaagaaaaa gaagaagcag aagaagaaga agaagaaaaa caagaagcgc aaaaagaaca
     4081 agaaggcaaa gaagcgctaa ggcccgcggg ctatggccaa gggagccatg atcctcgggg
     4141 aaggggcaag ccaaaccact gcgaatcagg catgattccg gtcatggcgg atcgttccag
     4201 acttgccacc gagcgcatga gcgtcagcat cgttcgcgcg gcgccgcgga tcgggcgtca
     4261 gcgcgttacg ttctgcgcgc tggagggatc tttgctgatc ctcatcgagg cggacagcga
     4321 ggatgaggcg cacgcgatct gcgccgagca agggctcgag tttcactcgc tctgcgaagg
     4381 ctgacggcgc ctggctggca aagcgtgcct agtccttctt gggcggcttc gactgcatgt
     4441 tgcggaaggc gtcggccatg agccaggaga gctgctgagc ctgaatgcca aaggtctgca
     4501 tctgagtctg cacgtagcgg ctctggagcg tcacgacgtc ctgcatatcc ttggcattgg
     4561 caagatcgct ggcgagcgag aaggaccgtt ccgcgttctc cttggcaaag gagacggcac
     4621 gctcttgcag ggccttgaag ttgcccgcct cgggggcgcc ggtcgacgac caggcattca
     4681 tagcttggct catgaactcc atgaactggc cataggccac gcgcgcctcc tcaacgttct
     4741 tctcagcgag ttcgcgcatt tcggtcggaa tttcgaacat cttattgtct ggcatcggtt
     4801 ccccttaagc ccggttttct tggcctcgcg gcctctcagc cggtcgtggt agtagcccta
     4861 gattacagca tttttcggca atcacactat ggtctgcgac cttaaggaag gcttgcctcc
     4921 aaggtctatg gcgggcttgg cgcttggtga aggccctgct tcgtggcacc aagcgcagca
     4981 atgacgcctc agaccgattc cccggcgcct ggacctgcgg aactgacacc gcgcgcgctt
     5041 gccaccggcc tcctccttgg cgcgctgctc gcgccctgca acgtctattc gggcctgaag
     5101 atcggctggt cgttcaacat gtcgatcacg gcgctgttgc tcgccttcgc cttctggatg
     5161 ccgcttgcgc ggctctgcaa gctcgcgcca tggggcatgc tcgaaagcaa catcaatcag
     5221 acgacggcct ccgcagcagc ggcgatcaca tcgagcggct tggtggcacc catcccggcc
     5281 ctggcggtga tgacgggcga gaacctgcat tgggccccgc tgacgatgtg ggtcttcact
     5341 gtgagctttc tcggcgtgtg ggtcggttgg tatttgcggc cgcgcatgat cgtgcaatcg
     5401 aacctcgtct tccccgcggg catggcgacg gcccagacca tgctggacat gttcgcgcat
     5461 aggcgagagg ccatggcgcg ggtgaaggtc ctgctgtcgg cgctgatcgg cgcggtcctg
     5521 ttcaacgtgg tcgataagct ggtgtggcac attccgcggc tggggccttc ggtgacagcc
     5581 tcgaagctca ccttcgtttt tgatccatcg cttctgttga tcggctttgg cggcattatc
     5641 ggcctgcgcg ccggccttgg cctcttgttc ggcgcgctga tcgcttgggg gctcattgcg
     5701 cctcaactga tcgcgcacga catcgttatc gtctcgcaag acgcgtcgaa ctggttcgag
     5761 ccgctcgtgg gctggcttct ttggcctggc gtcgccatga tgctggccgg aagccttaca
     5821 agcttcgggg cgtcgctgct cgacgtgtca aagccgcata gacgcccgca ggcccgcgcg
     5881 caaacttccg gcagcggcag gctgcagttc gccggcttcg tggccgccgc cgcgctcgcc
     5941 atcgcgcttc agatcatctt gttcgacgtg cactggacca tggcgcttgc gtccattgcc
     6001 attgcctttg cgctggccac tgtggcagcg cgggtcgtgg gcgaaacgtc cgtcgcgccc
     6061 atcggcgcga ttggcaagct gtctcagctc tcctttggtg tcatggcgcc cggtcagccc
     6121 atcaccaatt tgatgacggc gaatgtcgca ggcggcgccg caggacagtc ggcggatctc
     6181 ctgaacgatt tccgcgcggg ctacgaggtc ggtgcgcatc ccgcccgtca ggtcgcggcc
     6241 caatgcgcgg gcgtcgttgt ggggagcctg gtcagtagtc tcgtctacct gatgctgatc
     6301 ccggatccta cgacgcagct cttcacgccc gaatggccgg cgccggcggt cgcggtttgg
     6361 aaagccgtgg ccgaagcgct cagccatggg ctgaacggca ttgcgccgtc ggcgctcatc
     6421 gcgatggcca tcggcgcggt gctcggtata ggcctcgcgc tggcggagca ctggctggac
     6481 aagaaatatc tcctcttcgt gccgagcggg gccacgttgg gccttgcgtt cgtgattccc
     6541 gcaagcacgt cgctaatgct gttcgcgggc gccgcgcttg ccagtcttgc acacaagttt
     6601 gcgcccaact ggtccgcgcg gttcctcttg agcgtggcgg cagggctcgt ggcgggcgaa
     6661 agtctcttcg gcgttgtctc ggtatggttc taaaggctca tccgcacaga tatctcactg
     6721 gcaagccccg ggaggtcttg ttttgacgac cgtgacgact ggcatgattg gcggtggctt
     6781 ctataaccgc cactctgcgc cccaagcgaa tgcactcgaa tatgttctgc cttggctcat
     6841 cgaggccgcc gcggccatgg atctctcgcc ggtgcccgag gcggtcactc tggcggactt
     6901 cggatgctcg gaaggccaaa actcgatcaa ggtgatgcat gcggtgctgc cggtcttgcg
     6961 gtcgcgcacc agccggccgc tggtgacgat ccatagcgat ttgccgacca atgacttctc
     7021 cgaggcgctg ctggcgctgc ggccgcaagg ccagtcggtg ttcggagaga acacttattc
     7081 ggcgatcctc ggtggctcga tgttcgaccg gctcctgccg ccgcgctcac tccatgtcgc
     7141 catgacgttc aatgcggtcg gtttcttgag ccggcggccg ctcgatcgtc tgccgggtta
     7201 catcctgccg aatggaccaa gcgcgctgcg caatgtcggt gcggtgacag agaccgaaag
     7261 gagcgctttc gccgagcagg caggcgccga tctggagagc ttcttgcgtg cccgcgcggt
     7321 ggagcttgtg cccggtggga agctgttggt tcaggtgttc gggatgggtg aagagtatcg
     7381 cacctgtgat ggcctctacg atgtcctgaa cgatgcggtt ttggaggcgg ttacctccgg
     7441 tcagatcgat cgggcgacgt acgacgccta ctaccagccc gtctatttcc gcacgctcaa
     7501 agagctgctc acgcccttag aggcaggcac gggcgtgtgc accgacttgt tcagcatcga
     7561 ccgggccgag ttctatgagg tgccagtccc ctttgtcgaa gacttcaagc aaaacggcgc
     7621 cgccgcagcc tatgctagcg cttacacgcg cttcttgcgt gctttcaccg agccggtgct
     7681 gcgccttgcg ctaggtgaca atccaaagac ggatgcagtc gtcggcgatg tgttcggccg
     7741 tgtcgagcag ttgctgactg cgcatccgga gcgttaccgg tttgactatg tcagcgtggc
     7801 agcacttatg acccgcaccg gctagccgaa gggacgcgct actcggcggc ttcggcgtgg
     7861 gcttccatcg ccgccaaata gcgctccacg tcgagcgccg ccatgcagcc catgccggca
     7921 gcggtgacgg cctggcggta gacgtcgtcg gtcacgtcgc cggcggcgta cacgccctca
     7981 aggctcgtcg ctgtggagcc gggttcggtc ttgatgtagc cgccttgctt cagcgcgagc
     8041 tgatccttga acagctcggt ctgcggtgag tgaccgatgg cgatgaacac gccgtcgata
     8101 ggcatggtgg tctcatcgcc cgtcttggcg tttctgagct tggcggccgt gacgccaaga
     8161 ggatcctcat cgcccagcac ctcggcaagc tgatggtccc acagcacctc gatcttgggg
     8221 tgcttgaaca ggcgctcttg cagaatcttc tccgcgcgca aagcatcgcg ccggtgcacc
     8281 agcgtcacct tgtcggcgag gttggcgaga taaagcgctt cctctacggc ggtgttgccg
     8341 ccgccgacta cgatcacgtt ctttccgcgg tagaagaacc cgtcgcaggt ggcgcaggcc
     8401 gacacgccga agccctggaa cttgtcttca gagggaaggc caagccagcg cgccttggcg
     8461 cccgtggcga tgatcaccgc atcggcggtg tactcggtgc cgctgtcgcc tttgagcttg
     8521 aaggggcgct gcttgaggtc ggcctcgacg atggtgtcgg cgacgagatc ggtgccgaca
     8581 tgctcggctt gcgcgcgcat ctggtccatg agccaggggc cctggatcac gtcggcgaag
     8641 ccgggatagt tctccacgtc cgtggtgatg gtcatctgac cgccgggctg gatgccttcg
     8701 atcaggaccg gctccagcat ggcccgcgct gcatagatcg cggccgtgta gccggccgga
     8761 ccggcgccga taatgatcac cttggcgtga cgggaaccca tggaagaagc ctctcggttc
     8821 aaattaggaa gcaagacgta agcatgcccg ccgctctgcg caaccggctt tgggcgccgc
     8881 cgttttacgc tttggtttca agatcaagcg cctcgcgaat gcgcgcggcg accatttcgg
     8941 tgtcgtttat cggggcatag gtccacgatt cgaggtgccc ttcgaaggga cgcgtggccc
     9001 cacgctcgcg catcagggca tggaagcgcg caagtcgcgt cgagcggccg ggcatggcct
     9061 gcacatagac cggtttgccg gttcccgccg cttcggtgac catgttgacg gagtcctcgg
     9121 tgacgacgat ggcgtccgcc agcgcgagaa acgcgaaata ggggttgtag cccgtttcgt
     9181 cccaaacgaa gtggggcgtg tcggcgatcg ccttggatag ggcgtcaagc gactccacgc
     9241 gggtccgccg cgagggcgtg accagaagcg atccacccgt ttcgcgggcc atatccgcca
     9301 gcttcgcgcc aaaggctgcc gccgtctccg ggggaaagct gaaagcttgg ctctcgccgc
     9361 cgagctccac cgcgacacgg gggtggggca gcttcgcgat ggtgggcgca aaacgttcgc
     9421 ctgcttcagc gagccgttcc gcggtgacgc cgtggacagc gccgaaggtg gtgatgacgt
     9481 tcgggccttc gaaattgtcg tgggctgggg ccgcgaccag gtcgaagagg cgcgcgggca
     9541 ctttcggatt ttgaatgtga agggcgtagg cgcctgacag ccgcttgatc gccagtgcca
     9601 tgggaacgcc gcgccggccg atcgagatga ttagacgcgg ccaaggcggc tccagagcct
     9661 cgttactctt cgtttggcgg agtagggctt gtggcggcag gaggagctgc cccggcgcgg
     9721 gcaggagccg caggagccct ttgggggcga cccgcttcag ggcgtagggg aggccgacgg
     9781 cttcggccat ggcgatccct tgcgcctcca tgccgacgga cccatcggtg acgatccacg
     9841 tcttggccgt gcgggccttg cggctctcgg caatcagcgc ggcggcgcct tcggcatctc
     9901 cgccgggcgt gctctggggg tcgccaaaat ttccggaatg gggaacgttc aatttctttt
     9961 cgattctttt gcgaggaaga cgtaaagttg cgcaaagccg aaatttatcg aagcttgcgg
    10021 gctcattagg cgccttctag cgcgttgagc ctgtgatcgt ccagggagct tgagtaccca
    10081 tgcgcgccgc ccgcctcgat gccacggact ggttgattct gcgcgagctt caagcggacg
    10141 ggcggatcac caatattgag ctagcccgca aggtcgggat ctcggcgccg ccctgtctgc
    10201 gccgggtgcg ggcgctggaa gaagccggga tcatcgaggg cttcaccgcg atcctcaacg
    10261 ataaggcgct cggcttcgac gtttccgcct tcgccatgat caagctgcac agccaggccg
    10321 aggccgatct gattgccttt gaggaacggg tcgcggcatg cgcgatcgtg cgcgactgct
    10381 acatgctctc cggtgagatc gatttcctgc tgcgctgcgt ggcgccggac ttaagcgcct
    10441 tccaagagtt catcatccac gaactgaccg ccgcgccgaa cgtggagagc gtcaagacga
    10501 cgctcgtcat ccgcacctcc aagcacgccg cgggcgtgcc gacggagctc gtctccggcg
    10561 agatgcgggc gaagtagccg ttagcgcgcg acgttggcgt agaccgcgag attgcggtag
    10621 gcctcggcgc tctcgtgcgc caagtctggg ccgacaggcg ttcggccctt catggcgcgg
    10681 atcgcgccgt ccttggcgcc gtcgatcaga ttgtcggtga tcaacgcagg accggcggac
    10741 gggtcgaccg acaccgcgat gccgacatgg ctcttgcgga tcacgttgtc cgtgacgctg
    10801 acatcacgca aatacttggc ccaaccggcg aagatgccat aggccggcgc gccctcgatg
    10861 acgttgccgg tgacgacggt gtcggcctcg acgccgatgc cgacgccgcg aacctcgccc
    10921 tgcttccgca agaacagatt
//
LOCUS       DBNHVU010000046         5971 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_536390, whole genome shotgun sequence.
ACCESSION   DBNHVU010000046 DBNHVU010000000
VERSION     DBNHVU010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5971)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5971)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5971
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_536390"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..902)
                     /locus_tag="AC16Y7_01770"
     CDS             complement(54..902)
                     /locus_tag="AC16Y7_01770"
                     /inference="COORDINATES: protein motif:HMM:NF023413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR5708137.1"
                     /translation="MFDDRGHKVKLTGPASPVEILGLTSLPEAGDQFQVFPDSIKARQ
                     ISEFRQQKLRERELASSARLTLDQLHQKVQEGKIKELPIVLKADVQGSVEVLRDTLTK
                     LSNDEVKVKIIRAGAGAITDYDVLLASASNAVIVGFNVRPEPTARELAEKENIDIRLH
                     TVIYNITDELKKAMQGLLEPTYREVLLGHAEIRETFKVPKVGIIAGCYVTDGKVVRSA
                     DVRLLRDNVVIFEGKIGSLKRFKEDASEVKEGFECGIGLAGYNDIKVSDVIEIYTTEK
                     VSPSSD"
     gene            906..3515
                     /locus_tag="AC16Y7_01775"
                     /pseudo
     CDS             906..3515
                     /locus_tag="AC16Y7_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501101.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit A"
     gene            3517..3861
                     /locus_tag="AC16Y7_01780"
     CDS             3517..3861
                     /locus_tag="AC16Y7_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HIR5708138.1"
                     /translation="MELLVASAIGLITAVGVYLILQQRTFPIVVGLTLLSYAVNIFLF
                     AAGRLAVNQPPILTGADAYADPLPQALVLTAIVISFGMTALIVVLALRGFLETETDSA
                     DIPPDDLKADKR"
     gene            3858..5402
                     /locus_tag="AC16Y7_01785"
     CDS             3858..5402
                     /locus_tag="AC16Y7_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HIR5708139.1"
                     /translation="MTAGFTTDWVIAPAILPAMTAALLIVAWRRDIQMQRIVSVISAV
                     ALLGIALFLFDLASDGTVRAYRVGAWPAPFGIVLVLDRLSALMLLLAAGLALAVLIYA
                     LAGWDKRGRHFHALFHFQLLGLNGAFLTGDIFNLFVFFEVMLIASYGLMLHGAGARRL
                     RAGFQYVAINLVASTLFLIAVGLIYGITGTLNMADLAQKVPQVTAGNEALLKTGAILL
                     FLVFAIKAAFVPLHWWLPATYAAASAPVAALFAIMTKVGAYAIIRIFGTIFGDGAGAL
                     ANMVIPWILPAALLTLAVGTIGLLASRRLLDLAAFSIVASMGTLLIAISLFDVDGLTT
                     ALYYLVHSTLTGAALFLIVDLISERRGETLDWLVPANPISQAPILGTLFFLIALGVVG
                     LPPLSGFIGKLMVLDATYETRHVWLIWALVLVASLMMTISFARAGSVVFWKTDGRTTG
                     ATWATAAIAPMAAVAVLVTASAALSVFAGPAVEVFHATAVQALDPTGYIDAVLTSQPA
                     AQPTGQ"
     gene            5405..5896
                     /locus_tag="AC16Y7_01790"
     CDS             5405..5896
                     /locus_tag="AC16Y7_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HIR5708140.1"
                     /translation="MFKSLVPHPLLTLLILLVWMMLNSVSWGAAILGLTLGVAIPRLT
                     SAYWPSRPLISSPLTIVTYALIVLWDIVVANFQVAYLILFRRGDSLRSQFVTVPLDLR
                     TPEAITALAGTITMTPGTVSADLSGDGRALLVHCLETDDPEATVATIKSRYETRLKRI
                     FER"
BASE COUNT          912 a         2019 c         1742 g         1298 t
ORIGIN      
        1 ggtgcctagc tttcgtacgt gagtccccgc cggcggcgag ggctactccc gtctcaatcg
       61 gagctcggtg acaccttctc cgtggtgtag atttcaatca cgtcgctcac tttgatgtcg
      121 ttgtacccgg caagcccgat tccgcactcg aagccctctt tgacctcgct cgcgtcttct
      181 ttgaagcgct tcagacttcc gatctttcct tcgaagatga cgacgttgtc gcggagcagg
      241 cgcacgtcgg cggatcgaac caccttcccg tcggtcacgt agcaacccgc gatgattcct
      301 accttgggca ccttgaaggt ctcgcggatc tccgcatgtc cgagcaacac ctctcggtag
      361 gtgggctcga ggagaccctg catcgctttc ttgagctcgt cggtgatgtt gtagatgacg
      421 gtgtggagcc ggatgtcgat gttctccttc tcggccagct cgcgcgcggt cggctcgggc
      481 cggacattga agccgacgat cacggcgttc gatgccgaag ccaggaggac atcgtagtcg
      541 gtgatggcac ccgccccggc gcgaatgatc ttcaccttga cctcgtcgtt ggagagcttc
      601 gtcaacgtat cccggagcac ctcgaccgac ccctgaacat ctgctttgag gacgatcggc
      661 agctccttga tcttcccttc ttgaaccttc tggtgcagct gatcgagggt caaacgagcg
      721 ctcgaggcga gctcacgttc gcgcagtttc tgctggcgga actcggaaat ctgtctcgcc
      781 ttgatgctat cgggaaagac ctggaactgg tctcccgcct cgggcaagga ggtcaagcct
      841 agtatctcca cgggcgaggc aggccccgtc aacttcacct tgtgacctcg gtcgtcgaac
      901 atgcgctcat tctcggcatg ggcctgctga tcattctcta cgcgcgctac tatctcgggt
      961 ccgacgatcc gatggggctc ttctatttct atttgctgct gttccagggc gcgatggtcg
     1021 gcatcgtgct ctcggacaac attctgctgc tgatcgtctt ctgggagctg acgagcctca
     1081 cctcgttcct gctcatcggt tattggagcg atcaggccgc atcgcgtcag ggcgcgcgca
     1141 tggcgctcgt ggtaaccggc gcaggcgggc tcgcgctcat cggcgggatg ctgctgctcg
     1201 gccacatcgc cggctcctac gaaatttcag agatcctgca acgcggcgat gtcatccgct
     1261 cctcaccgct gctcttgccg acgctgctgc tcatcttagg cggtgccttc accaagtcgg
     1321 cgcagttccc gttccacttc tggctgccgc atgccatggc cgcgccaacg ccggtgtcgg
     1381 cctacctgca ttcggccacc atggtgaagg cggggctgtt tttgctagcg cggttctggc
     1441 cggtatttgc cagcaccgac gcctggtttc ttatcgtggc gccggtcggt ctcatcacca
     1501 tgctgatcgg cgcctggatt gcgctgttca gagacgacct caaggcgctt ctcgcctatt
     1561 cgacggtcag tcatttgggc ctcacgacca tgcttctcgg catggcgacg ccgctcgcgg
     1621 cgatcgccgc cgtgttccac attctcaatc acgcgacctt taaggccgca ctgttcatga
     1681 gcgcaggcat cgtcgaccac gaggccggca cgcgcgacat ccgcaaactc ggcggcctgc
     1741 tgacgctcat gcccatcacg gcgacgctcg ccttcatcgc ctctgcctcc atggccggca
     1801 tcccattgtt caacggcttt ctctcaaagg agatgatgct cgaagaggac tcgcatacgg
     1861 tctatgcggg gtatccgtgg atcttcccgg tcctggcgac actcgccgcg cttgtttcgg
     1921 ccgcctactc ctaccgcttc gccatcagca cgtttctggg caagcctcgc gactactatc
     1981 cgaagaagcc ccacgacccg ccggtcggca tgtggcttcc cgtcgccatc ctcatcgtgc
     2041 ccgtgatcgc catcggcatt cttccggcct tgacggccgg cgccatcgtc gaacgcacgg
     2101 cgctcgccgt cgtcggcgga caattgcccg agtacaagct tgcgctctgg catggcgtca
     2161 cacccgccct ttggatgagc gtgatcgcgc ttgccggcgg cgccttactg ttcaccgcct
     2221 accgccaggc cgaggccctg catcgcgtct ttgccggacc ggaggcaaag cggctctacg
     2281 atgcggcgat cgccgcgcta gtctccggcg cccgcgcctt cacggacgca acccacaacg
     2341 ggtccctgcc ccgctatgcg ggcatcatcc tcacggcgat cgtcgccgtc ggcgcaaccg
     2401 cgttcttgag cgcgccatac gcggcaggca cccgtgaggt tattccggtc gaatttcccg
     2461 cactcgccgc ttgggtggtc ctggcggcga tctgcgtcgc gctgctcttc tggcatcgcg
     2521 aaaggctgct tgccctcgtg ctgaccagtg gcgtcgggct gatcacctcg ctcgccttcc
     2581 tggagttctc cgcgccggac ctggcattga cgcaaatctc cgtcgacatc gtgacgacta
     2641 ttctgctgct cctcgccctc aatctcttgc cgaagacctc acgcggcgaa accccgcttg
     2701 ccggacgctt gggacgcggc acgctgtcgg cggcggttgg cctcggggtc ggtgccctcg
     2761 cctacgcgat catgacgcgc gacttccagt cgatctccga ctaccacctt gcggaagcca
     2821 aacctggggg cggcggcacc aatgtcgtca acgtcatcct cgtcgacttc cgtggttttg
     2881 atacgttcgg cgagatcatc gtcctcggca tcgccgcgat cacgatcttc gcgctgctgg
     2941 acacggcgct gaggggcgcc gccgcaaaga ccttagagtc gatccgctcg cccgagcaga
     3001 gcaaggacgc gcatccgcta atgctggttg tcgcgacacg cgtgctgttg ccgctggcgc
     3061 ttaccgtagc ggtcttcatc ttcctgcgcg ggcacaacca acccggcggc ggctttatcg
     3121 ccgggcttat cgtcgccgtc gccctcatca tgcagtatct ggcgagcggt tatgcctggg
     3181 cggcggagcg gaccagcgtc gacacccaaa ccgtgattgg cgcgggcgtc gccatcgccg
     3241 gccttgcggg ccttggctcc ttattcttcg gctggccgtt tctgacgagc acttacacat
     3301 atgtcgagct gccgcttatc ggcgagatcg agctcgcgtc cgccatcgct ttcgatctcg
     3361 gcgttttcct tgcggtcgtc ggcgtcgtga tgctgtctct cgcccaaatc tcgcgcgtcg
     3421 aggagcgcgc cgaacatgag cccgtccccg aaggccccat ggatgtgccg ctgaaaaaag
     3481 gccgcatcgt cagctcgaac gggcaggaga gctgacatgg agcttctcgt cgccagcgca
     3541 attggtttga tcaccgccgt cggggtctat ctgatcctcc agcaacggac cttccccatc
     3601 gtcgtcgggc tgaccctgtt gtcgtatgcg gtgaacatct tcctgtttgc cgcaggccgg
     3661 cttgcggtca accagccgcc gatcctcacc ggcgccgacg cctatgccga tccgttgcct
     3721 caagccctcg tgctgacggc catcgtcatt tcgttcggca tgacggccct gatcgtcgtg
     3781 cttgcgttgc gcggcttcct ggagaccgaa acggacagcg cggacattcc gccagacgat
     3841 ctcaaggcgg acaaacgatg acggcgggtt tcacgacaga ttgggtgatt gcccccgcca
     3901 ttctgccagc gatgacggcg gcgctgttga tcgtggcctg gcgccgcgac atccaaatgc
     3961 agcggatcgt gtccgtcatt tccgccgtgg cgctgctcgg catcgcgctg ttcctgttcg
     4021 accttgcgag cgacggcacg gttcgggcct atcgcgtggg cgcctggcct gcccccttcg
     4081 gcatcgtgct cgtgctcgac cgcctttcgg ccctgatgct gctgcttgcg gcggggctcg
     4141 cactcgcggt cctcatctat gcgctggcgg gatgggacaa gcgaggacgc cacttccacg
     4201 ctttgtttca tttccagctt cttggtttga acggcgcctt cctcaccggc gacatcttca
     4261 acctgttcgt gttcttcgag gtgatgctga tcgcctccta tgggctcatg ttgcatggcg
     4321 cgggcgcacg ccgcttgcgc gccggctttc aatacgtggc catcaacctc gttgcttcga
     4381 cgctgttcct gattgccgtc ggcctgatct acggcatcac cggcacgctc aacatggcgg
     4441 acttggccca aaaggtgccg caggtcacgg ccggcaacga agcactgctc aagaccgggg
     4501 cgatccttct gttcctggtc tttgcgatca aggccgcctt cgtgccgctg cattggtggc
     4561 tgccggcaac ttatgccgcg gcctcggcgc cggtggccgc cttgttcgcc atcatgacaa
     4621 aagtcggcgc ctacgcaatc atccgcatct tcggcacgat cttcggcgac ggcgcgggcg
     4681 cgctagcaaa catggtgatt ccctggattc tccccgcggc actcctcacg ctggctgtcg
     4741 gcacgatcgg gcttctcgcc agccgcagac tgctcgacct cgccgcgttc agcatcgtcg
     4801 cctccatggg cacgctgttg atcgccatca gcctgttcga cgtcgatggc ctgacgaccg
     4861 cgctctacta tctcgtgcac agcacgctca caggcgccgc gctattcttg atcgtcgacc
     4921 tcatctctga acggcgcggg gaaacgctcg attggctggt cccggcgaac cccatcagcc
     4981 aggccccgat cctgggcacc ttgttcttcc tcatcgcgct cggcgttgtc ggcctgccgc
     5041 cgctgtccgg cttcatcggc aaactcatgg tgttggatgc gacctatgag acgcggcatg
     5101 tctggctgat ctgggcgctg gtcctcgtcg cttctctgat gatgacgatt tcattcgcgc
     5161 gcgccggcag tgttgtattc tggaagacag acggtaggac caccggcgcc acctgggcaa
     5221 cggccgcgat cgcgccaatg gccgccgtag cggttctggt gacggcaagc gcagcgttga
     5281 gtgtctttgc cggtcccgca gtcgaagtat tccacgcaac ggcggtacag gcgctcgatc
     5341 cgacaggcta catcgacgcc gtgctgacca gccaacccgc cgcgcaaccg acaggacagt
     5401 gagcatgttc aaatcactgg tgccccatcc gcttctcaca ctgctcatcc tcctcgtctg
     5461 gatgatgctc aacagcgtgt cgtggggcgc ggccatcctc ggtctgaccc tcggcgtcgc
     5521 gatcccacgc ctcaccagtg cctattggcc cagcaggccg cttatcagca gcccgctcac
     5581 gatcgtcacc tacgccctca tcgtgctgtg ggacattgtg gtggcgaatt tccaggtcgc
     5641 ctatctgatc ttgttccgcc gcggagactc gctccgctcg cagttcgtga ccgtgccgct
     5701 cgacctcaga acgcccgaag cgattacggc gctcgccgga accatcacca tgacgcccgg
     5761 aacggtcagc gccgatctca gcggcgatgg gcgcgcactc ctcgtccatt gcctcgagac
     5821 ggacgacccg gaagcgaccg tggcgactat caagtcacgc tacgaaacca gactgaagag
     5881 gatcttcgag agatgatcga aattgcgtcc atgatcgggt tcggcgccat cggcgtggcg
     5941 atgctgttga gcctttaccg cttgctcgtc g
//
LOCUS       DBNHVU010000047         6486 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1429147, whole genome shotgun sequence.
ACCESSION   DBNHVU010000047 DBNHVU010000000
VERSION     DBNHVU010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6486)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1429147"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1662)
                     /gene="glmS"
                     /locus_tag="AC16Y7_01795"
     CDS             complement(<1..1662)
                     /gene="glmS"
                     /locus_tag="AC16Y7_01795"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR5708141.1"
                     /translation="MCGIVGVLGKGPVAELLVDALKRLEYRGYDSAGVATLENGQIGR
                     RRAEGKLRNLEELLKVEPLHGTSGIGHTRWATHGKPVEANAHPHMTDDVAVVHNGIIE
                     NFRELKAQLVAEGAPFSSDTDTEVIAHLITRELRAGKDPVAAVYQTLAHLQGAFALAM
                     IFRGYDDLMIVARQGSPLAIGYGDGEMFVGSDAIALAPFTDQVAYLEDGDWAVITREG
                     VAIHDRQGQSVERPVAKSLAAGLVVDKGNYRHFMAKEIHEQPEVVGHTLAHYLDLANY
                     QVSLPDLPLDFSQVTRLSLSACGTAFYSCFVAKYWFERWAKLPVDVDIASEFRYREIA
                     MDQSGAALFVSQSGETADTLASLRYCRDQRQHVLSVVNVQESSIARESDVVLPTLGGP
                     EIGVASTKAFTCQLTVLACLAIAAGKARGVIGPELEAELVKALVEVPRHMATILRDET
                     PYETLAHSLSKARDVLYLGRGTSYPIALEGALKLKEISYLHAEGYAAGELKHGPIALI
                     DEGVPVIVIAPRDNLFDKTVSNMQEVAAREGKIILVSDADPTSTGC"
     gene            complement(1771..3126)
                     /gene="glmU"
                     /locus_tag="AC16Y7_01800"
     CDS             complement(1771..3126)
                     /gene="glmU"
                     /locus_tag="AC16Y7_01800"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR5708142.1"
                     /translation="MTQSSALAIVLAAGKGTRMKSDLPKVLHRIAGAPMLAHVLQAGS
                     AAGIARGCLVVGPNMEAVGEAGQAVDAGLDVFVQPEQLGTADAVKAALPALEAGQGPI
                     LVLYGDTPLLRAETLKAVVGELDAGADLVVVGFEADDPTGYGRLLFDEEGRLAGIREE
                     KDASTAERALTLCNSGIVAFRSGETLKTLLARIGNDNAKGEYYLTDAVSLARADGLHA
                     RVVLSNAEEVLGVNSRAELAVAEAAMQMRLRNVAMAHGVTLIAPETVFFSYDTAIGRD
                     VVIQPNVIIGPGVTIDDGATIMGFCHFENARIGPGATVGPFARLRPGAELAKNAHVGN
                     FVEIKNAHVHEGAKVNHLTYIGDASVGAKANIGAGTITCNYDGFAKHRTEIGEGAFIG
                     SNSSLVAPVKVGDGAFVGSGSVVTKDVPEDALAVSRVPQQNHEGWAGKVRARRTRDKS
                     K"
     gene            3404..4453
                     /locus_tag="AC16Y7_01805"
     CDS             3404..4453
                     /locus_tag="AC16Y7_01805"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR5708143.1"
                     /translation="MVKRQRAMKRSLGRNTFAFLWIVLAGISGLYLYTLLTDPTALGG
                     QAERRAPSDGDTAAAPPPQRPPQQAVAAPPPADALSKADVAALKNTLQGLSRQVGQLD
                     ERLKPIERFVGPVAALPPSASVSTSPPKPQTPSPEPAPAAAVVEEVVEEVVEETPPPP
                     PPTPAPAPLAAAPEPAPEPGPAAAPAPEPEPEPAPEPAPAAAPAPEPEPAPPPVAAAP
                     EPAPEPAPAETAAATPPAPPAVPPAAVVETDVAAFDPVTLPPAANDGSTRYGIEIGAV
                     AKRDDLRPLWREYLTKHAALVAGLQPRRVLAPDKQWRLIAGPFANAQDAEGACSLFKK
                     ADRPCAATVYAGDAL"
     gene            complement(4473..5498)
                     /locus_tag="AC16Y7_01810"
     CDS             complement(4473..5498)
                     /locus_tag="AC16Y7_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5708144.1"
                     /translation="MEIGGSAYALDVLYYDDESTPQRGTVLVERLIREDGVRFILGPY
                     SALLTKAAVPVVEKHRIPMVEANGASRDVFSRGYEYLFAVLSTSDNYLTQAIDLAVAY
                     AAKFGKAPNELTLAMATDDGPLAQDARAGVLASVKEHGVNLVVDDRLPPELDDMSMTL
                     SKVEELKPDMLVVSGHDKGALTAVEQLGALQVSVPMIAMTHCDSAQVERKFLKASSYI
                     FCAQQWHRSLNYKGALFGSAEDFARAFEMVYGYAPPYQAAQSAAAVYVLADALERAQS
                     LDTDKVRDAIAATDLLTFYGPIKFDETGKNIAKRKILTQIIDGGYVVVAPEELAEQEP
                     VIPRPAQ"
     gene            complement(5819..>6486)
                     /gene="ypfJ"
                     /locus_tag="AC16Y7_01815"
     CDS             complement(5819..>6486)
                     /gene="ypfJ"
                     /locus_tag="AC16Y7_01815"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="KPN_02809 family neutral zinc metallopeptidase"
                     /protein_id="HIR5708145.1"
                     /translation="QAPQTSNEDDLKSFVAVVLGTTEDTWRQTFREIGAEYREPKLVL
                     FDDYVRSACGTGMSAMGPFYCPADEKVYIDLAFYRELKNRFRAPGDFAQAYVISHEVG
                     HHVQKLLGIADKVEGAKRRMSRRSANALQVRMELQADCFAGVWAHQMQADKGLIEPGD
                     IDEALRAASAIGDDRIQRRTQGYVVPDSFTHGSSEQRARWFRRGYADGRLQTCDTFNT
                     KQL"
BASE COUNT         1159 a         2165 c         2004 g         1158 t
ORIGIN      
        1 gcagccggtg ctggtgggat ccgcatcgct gaccaggatg atcttacctt cgcgcgccgc
       61 aacctcctgc atgttggaga ctgtcttatc gaacaggtta tcgcgcggcg cgatgacgat
      121 gaccggcacg ccttcatcga tgagcgcgat cggaccgtgc ttcaactcgc cggccgcgta
      181 gccttcggcg tgaaggtagg agatttcctt cagcttcaac gcgccttcga gcgcgatggg
      241 gtagctcgtg ccacgcccga gatacagaac gtcgcgtgcc ttggagagcg agtgcgccaa
      301 ggtctcatac ggcgtctcat cgcgcaggat ggttgccatg tgacgcggca cctcgaccag
      361 agccttgacc agctccgctt caagctccgg cccgatcacg ccacgcgcct ttccggcggc
      421 gatggcaaga caggcaagca ccgtaagttg gcaggtgaac gccttggtgg aggccacgcc
      481 aatctccggt ccgcccagtg tcggcagcac cacgtcggac tcccgggcga tagacgattc
      541 ttgcacattc acgaccgaga gcacgtgctg gcgctggtcg cggcaatagc gcagcgaagc
      601 aagcgtgtcg gcggtctcac cgctctgcga aacgaacagc gccgcaccgc tttggtccat
      661 ggcgatctcg cgataacgga actccgatgc gatatcgaca tcgacgggca gcttggccca
      721 gcgctcgaac cagtacttgg cgacaaagca ggaatagaac gccgtgccgc aggctgagag
      781 cgacagacgc gtcacttgcg agaagtcaag cggcaggtcc ggcagcgaga cctgatagtt
      841 ggccagatcg agataatgcg cgagcgtatg gccgacgacc tcgggctgct cgtgaatttc
      901 cttggccatg aagtggcggt agttgccctt gtcgacgact agccccgcgg ccaacgattt
      961 cgcgaccgga cgctcgaccg actggccttg ccggtcgtgt atggcgacgc cttcgcgggt
     1021 gatgaccgcc cagtcgccgt cctccagata ggcaacctgg tcggtgaacg gcgcgagcgc
     1081 gatggcgtcg gagcccacga acatctcgcc gtcgccatag ccgatggcga gcgggcttcc
     1141 ctgacgggcc acgatcatca agtcgtcgta accgcggaag atcatggcca gcgcgaacgc
     1201 gccctggaga tgcgcgagcg tttggtagac cgcggccacc gggtctttac ccgcgcgcag
     1261 ttcacgggtg atgagatgcg cgatcacctc ggtgtcggtg tcgcttgaga acggcgcccc
     1321 ctccgccacg agctgtgctt tcaactcgcg aaaattctcg atgatgccgt tatgcacgac
     1381 cgcgacgtcg tcggtcatgt gcggatgggc gttggcttcg accggcttgc catgcgtggc
     1441 ccagcgcgtg tgaccgatac cgctggtgcc gtgcagcggc tcgaccttga gcagttcttc
     1501 gagattgcgt agcttgcctt cggcacgccg ccggccgatc tgcccgtttt cgagcgtcgc
     1561 cacaccggcg gagtcgtagc cgcgatattc gagacgcttc agcgcgtcca cgaggagttc
     1621 tgcaacgggc cccttaccga ggacaccaac aatgccgcac atcgacccct actccctcga
     1681 tcccaaactc ttgcctaaac gggctgacgg catgtcgcaa ctcacgagct gtgtcccttt
     1741 attgcgattg aaattctaaa acacagcggc ctatttggac ttgtcgcgcg tgcgccgcgc
     1801 ccgcaccttg cccgcccaac cctcgtggtt ctgttgcggc acgcgggaaa cggcgagcgc
     1861 gtcctcgggc acgtctttcg taacgacgct tcccgaaccg acaaaggcgc cgtctccgac
     1921 cttgaccggc gcgaccagcg aactgttcga gccgatgaag gcgccctcgc cgatctcggt
     1981 acgatgtttg gcgaatccgt catagttaca cgtgatcgtt ccagcaccga tattagcctt
     2041 cgcgcccacg cttgcgtcac cgatgtaggt gaggtggtta actttggcgc cctcgtgcac
     2101 gtgcgcgttc ttgatctcga cgaagttgcc gacatgggca ttcttggcga gctccgcgcc
     2161 cggccgcagc cgcgcgaacg ggccgaccgt cgcaccaggt ccgatgcgcg cgttctcgaa
     2221 atgacaaaat cccatgatgg tcgcgccgtc gtcgatggta acaccggggc cgatgatcac
     2281 gttgggctgg atcaccacgt cacggccaat ggcggtgtcg taactgaaga acacggtctc
     2341 gggcgcgatg agcgtaacgc catgcgccat ggcgacgttg cgcagtcgca tctgcatcgc
     2401 ggcctcggca accgcgagct cggcgcgtga gttcacgcca aggacctctt cggcgttaga
     2461 caacacgacg cgtgcatgca agccgtctgc gcgcgccagc gacactgcgt cggtgaggta
     2521 gtattcgccc ttggcgttgt cgttgccgat gcgcgccagc agcgtcttca atgtctcgcc
     2581 tgaccggaac gccacgatgc cggagttgca cagagtgagt gcgcgctctg cggtgctcgc
     2641 gtccttctcc tcgcgaatgc ctgccaggcg cccctcctcg tcgaacagca gacggccata
     2701 accggtggga tcgtccgcct cgaagccgac cacgacaaga tcggcgcctg cgtcgagctc
     2761 gcccacgacc gctttgagcg tctcggcgcg caggagcggc gtatcgccgt acagcacgag
     2821 aatgggcccc tgcccggcct cgagggccgg caatgccgcc ttcaccgcat cggcggtgcc
     2881 gagctgctcg ggctggacaa atacatcgag gccggcgtcc acggcctgcc cggcctcgcc
     2941 gacagcctcc atattcggac cgacgaccag gcagccccgc gcaatccctg cggcgctgcc
     3001 cgcctgcagc acgtgggcga gcatgggggc cccggcgatg cggtggagca ccttcggcag
     3061 gtcggatttc atgcgggtgc ccttgcctgc ggcaaggacg atggcgagag ccgatgattg
     3121 ggtcatgccg tctttagaag tccctcttcc tggggcgggc ttgtcttggg gaaaactggc
     3181 cgccgcccaa cgttattcct gaccgccgtc tgtctaatcc aaagcgcgcc tcagggccac
     3241 agccccagca tctatgcgac cacctcgcgc aaaatcggca cagggaattt gaccagccag
     3301 atgaactgag taatctcgca aggagcggca cggcacaccc gattggggac ttcgttcagg
     3361 tggttatcgc gccgaaacct cgcatttgga ggctggattc ctcatggtca agcggcagcg
     3421 tgcgatgaag cggagcctag ggcggaatac gttcgccttc ttatggatcg tgctggccgg
     3481 tatttcgggc ctttatctct acacactgtt gaccgatccg acggcccttg gcggccaggc
     3541 tgagcgccgc gcgccctcag acggcgacac cgccgccgcg ccgccgccac aaaggccccc
     3601 gcaacaagcc gtggccgcgc ccccgccggc ggacgccctc agcaaggcgg acgtcgccgc
     3661 gctcaagaat acattgcaag gtctctcccg acaggtcggg cagcttgatg agcgcctgaa
     3721 gccgatcgag cgtttcgtcg gtccggtggc ggccctgccg ccgtccgcct cggtttcaac
     3781 ctcgccgccc aagccgcaaa cccccagccc agagcccgcg ccggccgccg ccgtggtcga
     3841 agaggtggtc gaggaagtcg tcgaagagac gccgccgcca ccaccgccga cacccgcccc
     3901 ggcaccctta gcggctgccc cggaaccggc gcccgagcct gggcccgccg ccgcccctgc
     3961 gcccgaaccg gaaccggaac cggcgcccga gcctgcgccc gccgccgccc ctgcgcccga
     4021 accggaacct gccccgccgc ccgtggccgc cgcgccggaa ccggcccctg agcctgcgcc
     4081 ggcggaaacg gccgccgcaa cgccccctgc accgccagca gtgccgcccg cagccgtggt
     4141 tgaaacggac gtcgcggcct tcgatccggt gaccctgcct ccggccgcca atgacggctc
     4201 gacgcgatac ggtatcgaga tcggtgccgt ggccaagcgc gacgaccttc gcccgctctg
     4261 gcgcgaatac ctgaccaagc atgccgctct ggtggcgggt ctgcagccgc gccgggtgct
     4321 cgcacccgac aaacagtggc gcttgatcgc cgggccgttc gccaacgctc aagacgctga
     4381 aggcgcgtgc tcactgttca agaaggcgga ccgtccgtgc gcggcgaccg tctacgccgg
     4441 cgacgcgctc taagtcgacc ggcctctacc tgctattggg ctggacgcgg gatcaccggc
     4501 tcttgctcgg cgagctcttc cggcgcgacc acgacataac caccatcgat gatctgggtc
     4561 aggattttgc gcttggcgat attcttgccg gtctcgtcga acttgatggg cccgtagaac
     4621 gtcagcaggt ccgtggcggc gatggcgtcg cgcactttgt ccgtatccaa gctttgtgcg
     4681 cgctctaacg cgtcggccaa cacatagacc gcggcggcgg actgggccgc ctggtatggc
     4741 ggtgcatagc cgtagaccat ttcgaaggcg cgcgcgaagt cttccgccga gccgaacaat
     4801 gcccccttgt agttgaggga gcgatgccat tgctgcgcgc agaagatgta cgacgacgcc
     4861 tttaggaact tcctctccac ttgcgcggaa tcgcaatggg tcatcgcgat catcggcaca
     4921 ctcacctgta atgcaccgag ctgctccacg gccgtgagcg cgcccttgtc gtggcccgac
     4981 accacaagca tgtccggctt gagttcttcg accttgctca aggtcatcga catgtcgtcg
     5041 agttccggcg gaagccgatc gtcgaccacc aagttaacgc cgtgctcttt tacgctggca
     5101 agcacgccag cgcgcgcgtc ctgcgcgagc gggccgtcgt cggtggccat ggcaagggtc
     5161 agttcgttcg gggccttgcc gaactttgcc gcatatgcca cggcgagatc gatcgcctga
     5221 gtcagatagt tgtcagacgt cgacaacacg gcaaagagat attcgtagcc cctggagaag
     5281 acatcgcgcg atgcgccgtt tgcctccacc atcggaatac gatgcttctc gacgaccggc
     5341 actgcagcct tcgttagcaa agcgctgtaa gggcctagaa tgaagcgaac gccatcctca
     5401 cggatcaaac gctcgacgag caccgtgcca cgttggggcg tggactcgtc gtcgtaatac
     5461 agaacgtcga gcgcgtaggc gctgccacca atctccaccc ccgcccttgt cgttgatttt
     5521 tcggaccgcg aggtcgtagc cgttcttggt gtgcgtgccg ctgacggcga acttgcctta
     5581 gagagaaagc gccgtgccaa gccgaatggt ctggtcggcg cttacggccc aaccagcact
     5641 gaatgcgagc gacgcaaacg cgatgcttgc gcagaatcga cgcgcgaacg ccatgtgatg
     5701 aaaccgcccc gtcttgcccg aatctcctgg agccgggaga ttacgcgcta atcggcccgc
     5761 gcgcatcttt tatgggcatt ttgcgacggc gtttctcgcc gagatcgccg aggccagact
     5821 agagctgctt ggtattgaag gtatcacaag tttgcagacg gccatccgcg tagccacggc
     5881 ggaaccagcg ggcgcgctgc tccgacgaac cgtgggtgaa ggaatctggc acgacatagc
     5941 cttgcgtccg ccgctgaata cgatcgtcgc caatggcgct cgccgcacgc agcgcctcat
     6001 cgatatcgcc cggctcgatg aggcccttgt cggcctgcat ctgatgggcc cagaccccgg
     6061 cgaagcaatc cgcttgaagc tccatgcgga cctgcaaggc gttggccgac cgccggctca
     6121 tccggcgctt ggcgccctcg accttgtcgg caattccgag cagcttttga acgtggtgac
     6181 caacctcatg ggagatcaca taagcctggg cgaaatcgcc cggcgcccga aagcggttct
     6241 tcagctcacg gtagaaagcg aggtcgatat agaccttttc gtcggcgggg cagtagaacg
     6301 gccccatggc cgacatgccc gtcccgcatg cggagcgcac atagtcgtcg aacaacacga
     6361 gcttgggctc ccgatattcg gcgccgatct cgcggaaggt ctgtcgccag gtgtcctccg
     6421 tggtaccgag cacgacggcg acgaaactct tcagatcgtc ttcgttggac gtttgtggag
     6481 cctgga
//
LOCUS       DBNHVU010000048        13521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_536885, whole genome shotgun sequence.
ACCESSION   DBNHVU010000048 DBNHVU010000000
VERSION     DBNHVU010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 13521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13521
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_536885"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="AC16Y7_01820"
     CDS             <1..385
                     /locus_tag="AC16Y7_01820"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014626.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HIR5708146.1"
                     /translation="YEWTGPKGKRRPFHIRPQPPRLIAFAGLYEYWQDPQGGEIDTAT
                     ILTCAANRTVSGLHDRMPVVVEPEHYDDWLDVTRVSAEEALAMIGPADDDLFETLELH
                     PKINDSRKDEPGIQEPLQPLQTSLL"
     gene            413..1297
                     /locus_tag="AC16Y7_01825"
     CDS             413..1297
                     /locus_tag="AC16Y7_01825"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009737156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HIR5708147.1"
                     /translation="MLSEKIIDRYRVTDGDKFKLKDYDPDDTGNVGFDKEDGREILEV
                     GVQKLARLQERLYAEGSWAILMVLQGIDTAGKDGVIKHVLSGVNPQGCSVHSFKQPSH
                     MELAHDFLWRCNLALPMRGNIGIFNRSYYEETIVVRVHPELLAKQNIPPKLVTKEIWK
                     ERYQDINAFERRLARAGTVPLKFFLNISKEEQLKRLLARVDDHEKNWKFSPYDVAERK
                     LWDKYQDVYEDTIKNTATKHAPWYVVPCDKKWFARLVVAGALIEHMKALDLHFPKVSD
                     EDMEALKKAHAELLAEKK"
     gene            complement(1304..2005)
                     /locus_tag="AC16Y7_01830"
     CDS             complement(1304..2005)
                     /locus_tag="AC16Y7_01830"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR5708148.1"
                     /translation="MTKKKFWDRTEDPNWEAIDSFIAEHFLEADPALEAALAASDAAG
                     LPPIQVDPLQGKLLTMLAAALGARNILEVGTLGGYSTIWLARGLTGGGRVITLDMNPE
                     YAEIARKNFATAGLADRIEVRVAPALDTLAALDAEGAGPFDLVFIDADKPNTPHYFDW
                     AVKLAREGSLIVVDNVVREGAILAAQSDSAHVKGLRDFYARVAADPRVSATAFQTVGS
                     KGHDGLAIVRVTQAP"
     gene            complement(2015..2965)
                     /locus_tag="AC16Y7_01835"
     CDS             complement(2015..2965)
                     /locus_tag="AC16Y7_01835"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR5708149.1"
                     /translation="MTALPATDQAIARAAQALARGETVAFPTETVYGLGANALDASAV
                     ARVFAAKDRPRFNPLIVHVPDIATAERYATFDATARKLAEAFWPGPLSLVAPKRPDSG
                     IADLVTAGLDTIALRAPDHPIAQKLLAATGLPLAAPSANRSGRVSPTTAAHVETELGD
                     LPAMILDGGPCARGIESTVVRVTGDAPVLLRLGAVPREAIEAVLGSPLPLVQDGTEIA
                     SPGQLERHYAPSTPLRLDAADVREGEALLAFGPNVPEGARETVNLSASSDLQEAATNL
                     FASLRALDACGAGTIAVMPIPDQGLGEAINDRLRRAAKAR"
     gene            3042..3968
                     /locus_tag="AC16Y7_01840"
     CDS             3042..3968
                     /locus_tag="AC16Y7_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR5708150.1"
                     /translation="MTTLLLTHPACLEHDTGYGHPERPDRLRAVNKALAAPAFNALAR
                     KKAPRADLAAIERIHPKAYVEMVKSEIPEQNHNWLDPDTVVSSGSWEAALRATGAVLH
                     AVDQVAEGKAKNAFCAIRPPGHHAEPSKAMGFCLFNSAAVAAMHARAAHGAKRVAVVD
                     FDVHHGNGTQAAFWEDKDAFYGSTHQMPLFPGTGALDETGVGNIFNAPLSPGDDGEKF
                     RAAYAERVLPGLDAFAPELLIVSAGFDAHLMDPLAQIRVLEPDFAWVTEKLMDVAEKH
                     CGGKLVSTLEGGYDLDALADSTAIHVKTLMGA"
     gene            4000..4254
                     /locus_tag="AC16Y7_01845"
     CDS             4000..4254
                     /locus_tag="AC16Y7_01845"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR5708151.1"
                     /translation="MADTDNADIKTMSFEQALKELETIVDRLEKGDVELEASIVIYER
                     GEALRAHCDGLLRKAEARVEKISLSQNGEPTGSEPLDVKD"
     gene            4402..6330
                     /gene="dxs"
                     /locus_tag="AC16Y7_01850"
     CDS             4402..6330
                     /gene="dxs"
                     /locus_tag="AC16Y7_01850"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIR5708152.1"
                     /translation="MSAETATPLLDKVRDPENLRGLGDDELKQLADELRRETVSAVSV
                     TGGHLGAGLGVVELTVALHAVFDTPRDKVIWDVGHQAYPHKILTGRRDRIRTLRQPDG
                     LSGFCKRAESEFDPFGAGHSSTSISAGLGMAVARDLAGGDNSVVAVIGDGAMSAGMAY
                     DAMNNAGAMDSRLIVVLNDNDMSIAPPVGAMSAHLARLISGGTYRSFRRWMKQVANKL
                     LPNSWFRRAARAEEFTRGFWTGGTLFEELGFYYVGPIDGHNLDHLLPVLRNVKKMKNG
                     PILVHVVTQKGKGYGPAEESADKYHGVGRFNVVTGTQEKAAPGGPPSYTKVFANALID
                     EARKDDKIVAITAAMPSGTGLDKFAQSFPERCFDVGIAEQHAVTFAAGLATEGYKPFA
                     AIYSTFLQRAYDQVVHDVAVQKLPVRFAIDRAGLVGADGPTHAGTFDTAFLSCLPDMI
                     VMAAADEAELVHMVATAAAIDDAPSAFRYPRGSGAGVALPEHGIPLEIGKGRIVREGS
                     TVALLSFGARLTECLGAADELAGYGLSATVADARFAKPLDIDLIRDLAKNHEVLITVE
                     EGSSGGFGGQVLHALSADGLLDRGLKVRTLVLPDRFIDQGKPEKMYADAALDAQGIVA
                     AVFAALGKDAKPGAARLA"
     gene            complement(6327..6797)
                     /locus_tag="AC16Y7_01855"
     CDS             complement(6327..6797)
                     /locus_tag="AC16Y7_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708153.1"
                     /translation="MTKRLLILLAAFVLASTEASAATNIWQAKSSGSFVTLRYGPLID
                     REHPRFLLSCLNSVKIAVLSVYMDFPERETGAAITVTFSAGGQSTTVAGETAAEDGTG
                     VIYGEAGDIAVAPILKVLREKGSVSMKSGAIGYDMSDAGRADAVAKFTKDCTLD"
     gene            7103..7831
                     /locus_tag="AC16Y7_01860"
     CDS             7103..7831
                     /locus_tag="AC16Y7_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006165049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR5708154.1"
                     /translation="MTKRRRIDQRLVEEWLVASRARAADLIRRGCVTVGVAKPGALVA
                     DEAAIAVAGDAGAHVSRGALKLLAALRSFGLSPHDAIALDVGASTGGFTEVLLAEGAA
                     KVYAVDVGRAQLHKRLRADKRVVSLDATDARKLNATLVPQSVTAIVADVSFISLTLAL
                     PAALKRAAPGAWLVALIKPQFEAGREAVGKGGIVRGEEDRMRAVKRVREFLEREGWES
                     IGVIDSPITGGSGNVEFLIGARKR"
     gene            complement(7878..8417)
                     /locus_tag="AC16Y7_01865"
     CDS             complement(7878..8417)
                     /locus_tag="AC16Y7_01865"
                     /inference="COORDINATES: protein motif:HMM:NF025135.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4166 domain-containing protein"
                     /protein_id="HIR5708155.1"
                     /translation="MRRPLFHRAYGEALGRLPPAIVRVHDTGNGRTWRGEANVTTGSS
                     LLARIVRALAALPPASGTVPILIHVQPDGDGETWRREFAGHPLTTHLRAGRTPGTIEE
                     ALWPVTAVLQLEPDKDGVQQVLIGLRLFGLLLPRFLWPDLDVRESAEGDRYRFRVVMK
                     LGGVPLEAYEGWLEAPEKA"
     gene            complement(8417..9007)
                     /locus_tag="AC16Y7_01870"
     CDS             complement(8417..9007)
                     /locus_tag="AC16Y7_01870"
                     /inference="COORDINATES: protein motif:HMM:NF016056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HIR5708156.1"
                     /translation="MRRHSCRADWRSDDGDRAGGPLSQAPRPDGPRLTAHVSGSRQAM
                     SVSVEPYSYRDDPAVPHFPDDGPLIVFDGVCVLCNGFARFVAKRDSAERFRFAEAQSA
                     LGGALFRHYGLDDIDFETNLLIQDGRAYGRLEAFARILGELGGPWSAARAALALPRPI
                     SDWLYDRIAKNRYAIFGRYETCPAKGDCLIARRLIG"
     gene            complement(8908..9999)
                     /gene="aroC"
                     /locus_tag="AC16Y7_01875"
     CDS             complement(8908..9999)
                     /gene="aroC"
                     /locus_tag="AC16Y7_01875"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR5708157.1"
                     /translation="MSHNSFGYLFRITTWGESHGPAIGCVVDGCPPGIPITETEIQAY
                     LDKRKPGQSKYTTQRKEPDTVEILSGVFETEDGEQVTTGTPISLLIKNVDARSKDYGD
                     IKDKFRPGHADYTYFQKYGVRDWRGSGRASARETAARVAGGAVARKVVPEMVVRGALV
                     QVGPHKIDRANWCWDTVGDNPFFCPDPKAVDTWAKFLDEVRKAGSSAGAVIEVVADGV
                     PPGLGAPVYGKLDQDICSGLMSINAVKGVEIGAGFAAAALSGEENADELRKHPPGWPE
                     FTSNNAGGILGGISTGQPVVARFVVKPTSSILTPRRTVDKDGNDTEVSTKGRHDPCVG
                     IRAVPIGEAMMAIVLADHYLRHRGQTGQG"
     gene            10243..10656
                     /locus_tag="AC16Y7_01880"
     CDS             10243..10656
                     /locus_tag="AC16Y7_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR5708158.1"
                     /translation="MRAAHAVFMSLTIALLAGAAPLTPAEAFPAFAGLTKKVEKPAKP
                     GRHSVGPRPSRWCGWWMRTQFGGGPEYNLARNWAKRGRGLNGPRVGAVVVWSHHVGYI
                     TGRNEAGQWIVKSGNDGGRVRERPRSVRGAVFRKI"
     gene            complement(10689..11858)
                     /locus_tag="AC16Y7_01885"
     CDS             complement(10689..11858)
                     /locus_tag="AC16Y7_01885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HIR5708159.1"
                     /translation="MRALRALYDSRYPGIADLKTRAKRRLPNFAFDYLEGGIDDEASK
                     RRNRQAFQDVHLTPRYLRDVSTVDTRVTLFGAGYALPLGVSPVGLGNMMWPGAEAALA
                     RAAQSARIPYVLSTFSTTLLEDIAQLAPDVCWFQLYVPRDEQVMEDLIARVKRAGFRA
                     LVVTVDVPVGAKRNRELKNGLKLPFTMTPRIVWQSLTHPTWALRTLTHGTPDFVNVTS
                     YRTDPSQGLSDFVTSMTRPGVPLDHLQRIRTLWDGPLIIKGLQAEENFRAAIDCGVDG
                     VIISNHGGRQLDAAPATLETLRQLPGDITDRLTVMIDSGVRTGLDIVRAKALGAHAAF
                     SGRSFFYGVGALGEAGGKQVIEITRDEITRSLQQLGCQRFEDMDASWLAEPCDKD"
     gene            complement(11854..12672)
                     /gene="fabI"
                     /locus_tag="AC16Y7_01890"
     CDS             complement(11854..12672)
                     /gene="fabI"
                     /locus_tag="AC16Y7_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIR5708160.1"
                     /translation="MADGGNLMAGKRGIVFGVANNRSIAWGITKAVTDQGAEVALTYQ
                     GEALKKRVEPLAAEVGSKLVLPCDVTDTASVDAVFETLAKEWGEIDFLVHAVAFSDKD
                     QLGGRYVDTTEENFTQTLLISCFSFTALAKRAEKLMPNGGSLLTLSYYGAEKVMPHYN
                     VMGVAKAALEASVRYLAADLGKNNIRVNGISAGPIKTLAASGISDFRYILKWNEYNSP
                     LRRTVTIDDVGGGGLYLLSGLSNGVTGEVLHIDAGYHVVGMKNEDAPDISVAKD"
     gene            complement(12761..12943)
                     /locus_tag="AC16Y7_01895"
     CDS             complement(12761..12943)
                     /locus_tag="AC16Y7_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708161.1"
                     /translation="MAQLDERGATPAIAAPALNADDKSRRFTPLRSYVTPARALGKDV
                     RRDPMRSPLIPLAQKS"
     gene            complement(12954..13259)
                     /locus_tag="AC16Y7_01900"
     CDS             complement(12954..13259)
                     /locus_tag="AC16Y7_01900"
                     /inference="COORDINATES: protein motif:HMM:NF028308.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HIR5708162.1"
                     /translation="MAVGPAPQGLQDLKASLDQSDRTIAFQALQMALNELGDGVTLKW
                     RRPSSQLSGRIKPVTAFRDDQGRICRQVVYSLARGDQKNEAQGIACRAQNGRWVIAG"
BASE COUNT         2435 a         4271 c         4375 g         2440 t
ORIGIN      
        1 ttatgaatgg accgggccga agggcaaacg gcgccccttt catataagac cccagccgcc
       61 acggctgatc gccttcgctg gtctctatga gtactggcag gatccgcaag gcggcgagat
      121 tgacacggcc acgattctca cttgcgcggc caaccgcaca gtctcgggcc tgcatgatcg
      181 gatgcccgtc gtggtggagc cggagcacta cgacgattgg ctggacgtca cacgcgtgag
      241 cgctgaagaa gcgcttgcca tgatcggccc cgccgacgac gatctcttcg agacactcga
      301 actgcatccg aagatcaacg attcgcgaaa agacgaaccg ggtatccaag aaccattgca
      361 gccattgcag acttcgctgc tctaggcccc aagggaccca cagggggaaa gcatgctgtc
      421 agagaagatc atcgatcgtt atcgcgtcac cgacggcgat aagttcaagc tcaaggatta
      481 cgacccggac gatacgggga atgtcggctt cgacaaggag gacggcaggg agatcctcga
      541 agtcggtgtc cagaaactcg ccaggcttca ggagcggctt tacgccgaag gcagttgggc
      601 cattctcatg gtcctgcaag gcatcgatac ggccggcaag gacggcgtca tcaagcacgt
      661 cctgtcgggc gtcaatccgc aaggctgctc ggtccattcc ttcaagcagc cgagccatat
      721 ggaactcgca cacgatttcc tttggcgctg caatttggcg ctgccgatgc gcggcaatat
      781 cggcatcttc aatcgctcct attacgagga gacgattgtg gtgcgcgtgc accccgagct
      841 gctggcgaag cagaacattc cgccaaaact cgtgaccaag gaaatttgga aagagcgcta
      901 tcaggacatc aacgccttcg agcggcgtct tgcgcgcgcc ggaaccgtgc cgctcaaatt
      961 cttcctcaac atctccaagg aggagcagct gaagcgcctg ctggcccgcg tcgacgatca
     1021 cgaaaagaat tggaagtttt cgccgtatga cgtggccgag cggaagctgt gggataagta
     1081 ccaagacgtc tacgaggaca cgatcaagaa caccgcgacc aagcatgcgc cgtggtacgt
     1141 ggtgccgtgc gacaagaagt ggttcgcccg gcttgtcgtt gccggtgcgc tgatcgagca
     1201 catgaaggcg ctcgacctgc acttccccaa agtcagcgac gaggacatgg aggccttaaa
     1261 gaaggcccac gcggagctgc ttgccgagaa gaagtaacgt tcgttagggc gcttgcgtca
     1321 cgcgcacgat cgccagcccg tcatggccct ttgagccgac ggtctgaaac gcggtcgcgc
     1381 tgacccgcgg gtcggcggcg acgcgggcat agaagtcgcg caagcccttc acatgcgcac
     1441 tgtcgctttg cgcggcgagg atcgcgccct cgcgcaccac gttgtcgacc acgatgagcg
     1501 atccttcgcg cgcaagcttc accgcccagt cgaaatagtg cggcgtgttc ggcttatcgg
     1561 cgtcgataaa cacgagatcg aagggcccgg cgccttccgc atcgagtgca gcaagcgtat
     1621 cgagagcggg cgcaacacgg acctcgatgc ggtcggcaag gcctgcggtg gcgaagttct
     1681 tacgcgcgat ctcggcgtac tccggattca tgtccagtgt gatgacccgg ccgcccccag
     1741 tgagccctcg cgccagccag atcgtgctgt agccgccgag tgtcccgact tcgaggatgt
     1801 tgcgcgcgcc gagcgcggcg gccagcatcg taagaagctt gccttgcagc gggtccacct
     1861 ggatcggcgg caggccggcg gcgtcgctgg cggctaaggc ggcctcaagc gcgggatcgg
     1921 cctcaagaaa gtgctcggcg atgaagctgt cgatcgcctc ccagttcggg tcttcggtcc
     1981 ggtcccagaa cttcttcttg gtcatggtga gcccctagcg cgctttggcg gcgcgccgca
     2041 agcggtcgtt gattgcctcg cccaaacctt ggtcggggat tggcatcacc gcgatggttc
     2101 ctgcgccgca cgcatcgagc gcgcgaagcg aggcgaagag gttggtggcc gcctcttgca
     2161 gatcgctgga ggcgctgagg ttgacggtct cgcgcgcgcc ctccggcacg ttcggtccga
     2221 aggctagcag cgcttcacct tcgcggacat ccgccgcgtc aagccgcaac ggtgtgctgg
     2281 gcgcgtagtg ccgctccaat tgtccggggg aggcaatctc ggtgccgtct tggacgaggg
     2341 ggaggggact gccgagcaca gcttcgatgg cctcgcgcgg cacagccccg agccgaagca
     2401 agacaggcgc atcgccggtg acgcgcacca cggtcgactc gatcccacgc gcgcaaggac
     2461 ccccgtcgag gatcatggcg gggaggtcgc ccaactcggt ctccacatga gccgccgtgg
     2521 tcgggctgac gcggccggag cgattggcgc tcggcgcggc cagggggagc ccagtcgccg
     2581 ccagtagctt ttgcgcgatc gggtgatcgg gcgcgcgtag cgcaatcgta tcgagaccgg
     2641 ccgtgaccag atcggctatc ccgctatcgg gacgcttggg tgcgaccagc gacagtgggc
     2701 cgggccagaa cgcctcggcg agcttgcgtg ccgtggcatc gaaggtggcg tagcgttcgg
     2761 ccgtggcgat gtcggggacg tgcacgatga gcgggttgaa gcgcggccgg tccttggcgg
     2821 caaaaaccct cgcgacggcc gaggcgtcga gcgcgttggc gccgagccca tagaccgtct
     2881 ccgtgggaaa ggcgaccgtc tccccgcggg caagcgcctg cgcggcacgg gcaatggcct
     2941 gatctgtggc ggggagggct gtcatggtcg gcctcctttg gcgcgttctc aagggacggt
     3001 caagctgtcg tctgggcctc agtcgggtat catgccccga catgaccacg ctgcttctga
     3061 cccatcccgc ctgcctggaa cacgatacgg gctatggcca ccccgagcgc ccagaccgct
     3121 tgcgcgccgt aaacaaggcg ttagccgcgc cggctttcaa cgcgctcgcc cgcaagaagg
     3181 cgccgcgcgc cgacctggcc gcgatcgagc gcatacaccc caaggcctat gtggagatgg
     3241 tcaagtccga gatcccggag cagaaccaca actggctcga tcccgacacg gtggtgtcgt
     3301 ctggaagctg ggaggcggcc ttgcgcgcca ccggcgccgt gctgcacgcg gtcgaccagg
     3361 tggcggaggg caaagccaag aatgcgttct gcgccattcg tccgccgggc catcacgccg
     3421 agccgtcgaa ggccatgggc ttttgcctgt tcaactccgc ggccgtcgcc gcgatgcacg
     3481 cccgcgccgc ccatggggcc aagcgtgtcg ccgttgtcga ttttgacgtg catcacggca
     3541 acggcaccca ggccgctttc tgggaggaca aggacgcgtt ctacggttcg acgcaccaaa
     3601 tgccgctatt ccccggcact ggcgcgctcg acgagacggg cgtcggcaat atcttcaacg
     3661 cgccgctctc gccgggcgac gacggggaga agttccgcgc cgcctatgcg gagcgcgttc
     3721 tgccggggct cgatgccttc gcccccgagc tcttgatcgt ctccgccggg ttcgacgcgc
     3781 atttgatgga cccgctggcg caaataaggg tgttggagcc tgatttcgcc tgggtcaccg
     3841 agaagctcat ggacgtcgcc gagaagcatt gcggcggcaa gctcgtctct acgctcgagg
     3901 gcggctacga cctggacgcg ctcgccgatt ccaccgccat ccacgtcaag acgctgatgg
     3961 gcgcttgatt aagccccgcc gaaggggcta agccaggcca tggccgatac cgacaatgcc
     4021 gatatcaaga cgatgagctt cgagcaggcg ctgaaggagc tcgaaaccat cgtcgatcgc
     4081 ctggagaagg gcgacgttga gctcgaggcc tccatcgtca tctacgagcg gggtgaggcg
     4141 ctgcgcgccc attgcgacgg gttgctgcgc aaagcggaag cccgtgtgga gaagatctcc
     4201 ctcagccaga acggcgagcc tacgggctcc gagcccctcg acgtcaagga ctagcggcgg
     4261 ccgtttcacg ataaacgcct tgggccggac ctgtctgtcc gctaaggtcc aacgtcttgc
     4321 tgccgggccc gccgagggcc tcataagata taacgtgctg atcctttgag gcctttttcc
     4381 caatagtcgc cagggcaatc aatgagcgcc gagactgcca cgccattgct ggacaaggtc
     4441 cgtgacccgg agaatttgcg tggcttgggc gacgacgagc tgaagcagct tgccgacgaa
     4501 ctgcgccggg agaccgtcag cgcggtgtcc gtcacgggcg gccatcttgg cgccgggctt
     4561 ggcgtggtcg agctgaccgt ggcgctgcat gcggtcttcg acacgccccg cgacaaagtc
     4621 atctgggatg tcggccatca ggcctatccg cacaagattc tcaccggccg ccgcgaccgc
     4681 atccgcacac tgcgccagcc cgacggcctg tcgggtttct gcaagcgcgc cgagagcgaa
     4741 ttcgatccct tcggcgccgg ccattcctcc acgtcgatct cggcggggct tggcatggcc
     4801 gtggcccgcg atcttgccgg cggggacaac agcgtggtcg ccgtgatcgg agatggcgcc
     4861 atgagcgccg gcatggcgta cgatgccatg aacaatgcag gcgccatgga ttcgcgcctg
     4921 atcgtcgtcc tcaacgacaa tgacatgtcg atcgccccgc ccgtgggcgc catgagcgcg
     4981 catctggcgc gcctgatctc gggcggcact tatcgcagct tccgccgctg gatgaagcag
     5041 gtggcaaaca agcttctgcc gaacagctgg ttccgccgcg ctgcgcgcgc cgaggagttc
     5101 acgcgcggct tctggaccgg cggcacgctg ttcgaggagt tgggcttcta ctatgtcggg
     5161 cccatcgacg ggcacaatct cgatcacttg cttcccgttt tgcgtaacgt gaagaagatg
     5221 aagaacgggc cgatcctcgt tcacgtcgtc acgcagaagg gcaaaggcta cggcccggcc
     5281 gaagagtccg ccgacaagta tcacggcgtc ggccgcttca atgtcgtgac cggcacgcag
     5341 gagaaggccg cgcctggcgg tccgccgagc tacaccaaag tgttcgcgaa cgcgctgatt
     5401 gacgaagcgc ggaaggacga caagatcgtc gccatcaccg cggcgatgcc gtcggggaca
     5461 ggtctcgaca agttcgcgca aagctttccc gagcgctgtt tcgatgtcgg catcgccgaa
     5521 cagcacgcgg tgacctttgc cgcagggttg gcgacggaag gctataagcc cttcgccgcg
     5581 atctactcga cattcctgca gcgcgcctac gatcaggtgg tgcatgacgt tgcggtgcag
     5641 aagctgcctg tgcgcttcgc catagaccgc gccgggcttg tgggcgccga cgggccgact
     5701 cacgcgggca cgttcgacac ggccttcttg tcgtgcctgc cggacatgat cgtcatggcc
     5761 gccgccgacg aggcggagct ggtgcacatg gtggcgaccg ccgccgccat cgacgatgca
     5821 ccgtccgcgt tccgttatcc gcgcgggagc ggtgcaggcg tcgcactgcc ggagcacggt
     5881 ataccgcttg agatcggcaa gggccggatc gtacgcgaag gttccaccgt ggcgctgctt
     5941 tcgttcggcg cgcggctgac cgagtgcctc ggcgccgcgg atgagcttgc aggctacggt
     6001 ctgtcggcca ccgtggcgga cgcgcgtttc gccaaaccgc tggacattga tctcattcgg
     6061 gacctcgcca agaaccacga agtgctcatc accgtggagg aaggctcaag cggcggcttt
     6121 ggcggccaag tgctgcacgc gctttcggcc gacggcctgc tcgaccgcgg gctaaaagta
     6181 cgcaccctcg tgctgccgga ccgcttcatc gatcagggca agccggagaa gatgtacgcg
     6241 gacgcggcgc tcgatgcgca aggcatcgtc gcggcggtat tcgccgcgct cggcaaggac
     6301 gcaaaacccg gcgcggcgcg cctggcttaa tccagcgtgc aatccttggt gaatttggcg
     6361 acggcgtcgg cgcgtcccgc atcggacatg tcgtatccga tcgcgccgga tttcatcgat
     6421 acggatccct tttcgcgcag gactttgaga atgggcgcaa cggcaatgtc gccggcctcg
     6481 ccatagatca cgcccgtgcc gtcttcggcc gcggtctcgc ccgccaccgt cgtcgactgt
     6541 ccgccggcgc tgaacgtgac ggtaatcgcc gcgcccgtct cccgttccgg aaagtccata
     6601 tacacggaca gcacggcgat cttgacgctg ttcaagcagg acagcaggaa gcgcggatgc
     6661 tcacggtcaa tgagcggccc gtagcggagc gtcacgaagc tgccgctcga ttttgcctgc
     6721 cagatattcg ttgcggcgct ggcctccgtg cttgcaagca caaaggcggc cagaagaatg
     6781 agaagacgct tcgtcacggg acctcgcaag gtgcggtttc gatggcggct cttgcttagt
     6841 cgacccgaag cgtcttggca aacttcggcg ttttttggtg ccgcgcgctc aagcttcaag
     6901 ccgtcgatac gccactgaag gtaaggcgat aagtggcccg cataacgttg ccataaagcg
     6961 ggattgcgcc agtccttgcc cgtcacctcg ccccggcgcg atgccgaacc tcgcatttgc
     7021 gggtaaggta tgtgtcttgg ctcaaagtgc ctccgcgctc gcgtgtccac gcgtgaatgc
     7081 ggaccatata tgagaacagc ccttgaccaa aagaagacgc atcgatcaac gcctggtgga
     7141 agagtggctc gtcgcctccc gcgcccgcgc cgcggacctc atccgccggg gctgcgtcac
     7201 tgtgggcgtt gcgaagcccg gcgcgctcgt ggccgacgag gcggcgattg ccgttgccgg
     7261 cgacgcaggc gcgcacgtct cacggggtgc gctcaaactt cttgccgcgc tgcgttcgtt
     7321 tggtctgtcg ccgcatgatg ccattgcgct cgatgtcggc gcctcgaccg gtgggttcac
     7381 cgaagtgctg ttggcggaag gcgccgcgaa ggtctacgcg gtcgatgtcg gccgcgcgca
     7441 gctccataag cgtctgcggg ctgacaagcg cgttgtgtct ttggatgcga cggacgcgcg
     7501 caagctcaac gcgaccctcg tccctcagtc cgtcactgcc attgtcgccg atgtcagctt
     7561 catcagtttg acattggcgc tgccggccgc gctgaaacgt gccgccccag gtgcttggct
     7621 tgtggcactg atcaagccgc aatttgaggc ggggcgcgag gcggttggca aaggcggtat
     7681 cgtgcgcggc gaagaagacc gcatgcgggc cgtcaagcgc gtgcgcgaat ttctcgaacg
     7741 agaaggctgg gagagcatcg gcgtgatcga ctcccccatc accggtggca gcggcaatgt
     7801 ggaattcctg atcggcgcgc ggaagcgcta gcctgcggaa agactagttc ccgtcagaat
     7861 gacgatacgc tcgacgttca tgctttctct ggcgcttcga gccacccttc atacgcttcg
     7921 agtggcacac cccccagttt catgacaact ctaaatcgat aacggtcgcc ctcggcgctc
     7981 tcgcgtacgt caaggtcagg ccatagaaag cgaggaagta gcaagccgaa cagacgaagc
     8041 ccgattagca cctgctggac cccatctttg tcaggctcaa gctgcaaaac cgcggtcacc
     8101 ggccagagcg cttcttcgat cgttcccggc gttcggcctg cgcgcaagtg tgtggtgagc
     8161 ggatggccgg caaattcgcg gcgccatgtt tcaccgtccc cgtcgggctg cacgtggatc
     8221 aggatgggca cggtccctga cgccggtggc agggctgcga gagcccgaac gatgcgcgcg
     8281 agcagggagg aaccggtcgt cacgttcgcc tcgccgcgcc aagtccgccc gttgccggta
     8341 tcgtggacgc ggacgatggc tggaggaagc cgtccgaggg cctcgccata agcgcggtgg
     8401 aacaatggtc ggcgcatcaa ccgataagcc tcctggcgat caggcaatct cccttggctg
     8461 ggcaggtttc atagcggccg aatatggcgt agcgattctt ggcaatacga tcatagagcc
     8521 agtcgcttat tgggcgtggc agggcaagcg cagccctggc cgcggaccat gggcccccga
     8581 gctccccaag gatgcgggcg aaagcctcga gccgtccgta ggcgcggcca tcctgaatca
     8641 aaagattcgt ctcgaagtcg atgtcgtcga gaccataatg gcggaaaaga gcacccccga
     8701 gagcggactg cgcctcggcg aagcgaaagc gttccgcgct atcgcgtttg gcgacgaacc
     8761 gggcaaaccc attgcataag acgcaaactc catcgaacac gatcagcggc ccatcgtcgg
     8821 gaaagtgcgg aaccgcagga tcatcgcgat aggagtacgg ctcaacgctg acggacatgg
     8881 cttgccggct gccactcacg tgcgctgtca gccttggccc gtctggccgc ggtgcctgag
     8941 atagtggtcc gccagcacga tcgccatcat cgcttcgcca atcggcacgg cacgaatgcc
     9001 gacgcatgga tcgtggcgtc ctttggtcga gacctcggtg tcgttgccgt ccttgtcgac
     9061 cgttcgccgt ggcgtgagaa tggacgaagt aggcttcacc acgaaccgcg cgacaacggg
     9121 ttgccccgtc gagatgccgc cgagaattcc accggcgttg ttcgacgtga actcgggcca
     9181 gccgggaggg tgcttgcgca actcatcggc gttctcttcg cctgagagcg cggcggccgc
     9241 gaagcctgcg ccgatttcaa cgcccttcac cgcgttgatg ctcatcaggc cggaacagat
     9301 gtcctggtcg agcttgccgt agacgggcgc gccgagtcct ggcggcacgc cgtcggccac
     9361 gacctcgatc accgcgccgg ccgaagaacc ggctttgcgc acctcgtcga ggaacttggc
     9421 ccaagtgtcg accgccttcg ggtcggggca gaagaacggg ttgtcgccaa ccgtgtccca
     9481 gcaccaattt gcgcggtcga ttttgtgcgg accgacttgc acgagggcgc cgcgcacgac
     9541 catctccggg accactttcc gcgccacggc gccgcctgcg acgcgcgccg ccgtttcacg
     9601 cgcggatgca cggccggagc cccgccaatc gcgcacgccg tatttctgga aataggtgta
     9661 gtcggcgtgg ccggggcgga acttgtcctt gatgtccccg tagtccttgg accgcgcatc
     9721 gacattcttg atcagcagcg agatcggcgt gccggtcgtg acctgttcgc cgtcctcggt
     9781 ctcgaacacg ccggagagga tctcgaccgt gtcgggctcc ttgcgctggg tcgtgtattt
     9841 cgactgacct ggcttgcgct tgtccagata ggcctggatc tccgtctcgg tgatcgggat
     9901 accgggcggg cagccgtcga ccacgcagcc gatggccggc ccgtggctct cgccccaggt
     9961 ggtaatgcgg aaaaggtatc cgaagctgtt gtgcgacatg gcgtctctct agccgcttct
    10021 agtttagggg gcgtgaaggg tcaaacggcg caagcttggg gattttgagg gaataggggc
    10081 gccaacagcc ccgccaggcc cgaaaattgg cccatcctgg ccgtctccgg ggccagtttt
    10141 cgtcttattt ttccgtagtt tgcgcctccg cgccaatgaa gcgcggaccg gccgagacct
    10201 cgccggtcat cccctcccaa gacaaaaaac aaggagtatt cgatgcgtgc tgcgcatgct
    10261 gttttcatga gtctgaccat tgccttgctg gcgggcgctg ccccgctcac cccggctgag
    10321 gctttccccg cgttcgcggg gctgaccaag aaagtcgaga aacccgcaaa acccgggcgc
    10381 cacagcgtgg ggccgaggcc atcccgctgg tgcggctggt ggatgcggac ccagttcggc
    10441 ggcggacctg aatacaatct cgcccggaac tgggccaagc ggggccgggg cctgaacggt
    10501 ccgcgcgtcg gcgccgtggt cgtctggtcg caccatgtgg gctacatcac cggccgcaac
    10561 gaagccggcc agtggatcgt gaagtcgggc aatgacgggg gccgggtgcg cgagcgtccg
    10621 cgctcggtta gaggcgccgt cttccgcaag atctaagcaa acggggcagg gcgtgttcgc
    10681 gtcctgccct aatccttatc gcaaggctcc gcgagccagc ttgcgtccat gtcttcgaaa
    10741 cgctgacatc caagctgctg cagactgcgc gtgatttcgt cgcgagtgat ttcgatcacc
    10801 tgcttgccgc cggcctcgcc gagcgcgcca actccgtaga agaaactgcg gccgctgaag
    10861 gcggcatgcg ctccaagcgc tttagcgcga acgatgtcca gcccggtacg gacaccgctg
    10921 tcgatcatca cggtcaaccg gtcggtgatg tcgccgggca actgccggag cgtctcaagc
    10981 gtggcaggcg cggcatcgag ctgtcggcca ccgtgatttg agatgatgac gccatccacc
    11041 ccgcagtcaa tcgccgcgcg gaagttctcc tccgcttgaa gtcccttgat gatcagcggc
    11101 ccgtcccaca gagtgcgtat gcgctgcaag tggtccagcg gcacgcccgg acgggtcatg
    11161 ctggttacga agtcgcttag accttggctt gggtctgtcc ggtagcttgt cacgttgacg
    11221 aaatccggtg tgccatgtgt cagcgtccgg agcgcccagg tcggatgcgt cagggactgc
    11281 cagacgatgc gtggtgtcat cgtgaacggc agtttcaggc cattcttcag ttcccggttg
    11341 cgcttggcgc ccaccgggac atccaccgtg accaccaagg cccgaaagcc tgcgcgcttc
    11401 acgcgtgcga tgaggtcctc catgacctgc tcatcccgcg ggacatagag ctgaaaccag
    11461 cacacgtcgg gcgccaactg cgcgatgtcc tcgagcaagg tggtgctgaa cgtactgagg
    11521 acgtagggga tgcgcgcgct ctgcgcggca cgggccagtg ccgcttctgc gcccggccac
    11581 atcatgttgc cgagcccgac cggcgataca ccgaggggca gcgcgtatcc ggccccgaac
    11641 agcgtgacgc gcgtgtcgac agtgctgaca tcgcgcaagt aacgcggcgt caagtgcaca
    11701 tcttggaagg cttgtcggtt tcgtcgcttt gacgcttcgt cgtcgatccc gccttccaga
    11761 tagtcgaagg caaagtttgg caggcggcgt tttgcgcggg tcttgagatc ggcaatgccc
    11821 ggatatcggc tgtcgtaaag cgcgcgcagc gccctaatcc ttcgccacgg agatgtccgg
    11881 cgcgtcctcg ttcttcatgc cgaccacgtg gtagccggcg tcgatatgca ggacctcgcc
    11941 ggtgacgccg ttcgacagac ccgacagcag gtaaagtccg ccgccgccca cgtcgtcgat
    12001 ggtcaccgtg cgccttaagg gcgagttgta ctcgttccac ttcagtatat agcggaagtc
    12061 ggaaatgccg gacgccgcca gcgtcttgat cggcccggcc gagatgccgt tgacgcggat
    12121 attgttcttg ccgagatctg ccgcgagata gcgcacgctt gcttcaagcg ccgccttggc
    12181 cacgcccatc acgttgtaat ggggcatgac cttctctgcg ccgtaatagc tcagcgtcag
    12241 aagcgagccg ccattcggca taagcttctc ggcgcgtttt gccagcgccg tgaaggagaa
    12301 gcaggagatc agcagggtct gggtgaagtt ctcttcggtg gtgtccacat agcggccgcc
    12361 gagctggtct ttgtcggaga aggcgacggc gtggacgagg aaatctattt cgccccattc
    12421 cttagcgagc gtttcgaaca cagcatcgac cgaggcggtg tcggtcacgt cgcaaggcag
    12481 cacgagtttt gagcccacct cggcggcgag cggctcgacg cgcttcttca aggcctcgcc
    12541 ctgataggtc agcgcgacct cggcgccttg atccgtcacg gctttggtaa tgccccaggc
    12601 gatggaacga ttattggcga cgccgaaaac gatgccgcgc ttgcctgcca tgagatttcc
    12661 gccgtcggcc atgacccctc ctccgagcct caagaattcg gggtcttata caccgggcga
    12721 agctcacggt aagggctttc gccggatcga ggctaacggc ctaggatttt tgagcgaggg
    12781 ggatgagcgg cgagcgcatc ggatcgcgcc tgacgtcctt gcccagcgcg cgtgccgggg
    12841 tcacataaga ccggagcggg gtgaaacggc ggctcttgtc gtccgcattg agggcaggcg
    12901 ccgctatggc gggcgtcgcg cccctctcgt caagctgagc cataaagacc tctctagccg
    12961 gcgatgaccc agcgcccgtt ctgcgcacgg caggcgatgc cttgggcctc gttcttctgg
    13021 tcaccgcggg ccagcgaata aaccacctgg cggcagatac ggccctgatc gtcccggaag
    13081 gcggtcacag gcttgattcg gcctgaaagc tggctggagg gccggcgcca tttgagcgtc
    13141 acgccgtcgc cgagctcgtt cagcgccatt tgcagcgcct ggaacgcgat cgtgcgatcg
    13201 gactgatcga ggctggcttt gaggtcctga aggccttgcg gcgcagggcc aacggccacc
    13261 accgcagttt tttgtgcccc actctccccg gccataaggg gagaaagggc ggggagcaat
    13321 acagacaaca cgagagcccc cgccccgatg ataggggcgt tccaatggaa cgtacgcata
    13381 acaaactcgt ccccaaacag tgacgcggct actgaattcc cccgcgccct ctatcaacgg
    13441 gatcgatcct gcactatcag ggtgaggaaa tcgtaaatgg ccgtatcggg ccgggaattt
    13501 tttttgaaat cccttgcgcg c
//
LOCUS       DBNHVU010000049         5041 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_463474, whole genome shotgun sequence.
ACCESSION   DBNHVU010000049 DBNHVU010000000
VERSION     DBNHVU010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5041)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5041)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5041
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_463474"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2529
                     /locus_tag="AC16Y7_01905"
     CDS             <1..2529
                     /locus_tag="AC16Y7_01905"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HIR5708163.1"
                     /translation="SIAEQNAAMRRRRHIEQTRGRDKLRGTFDHVLAEAAADGIAPKE
                     VDDLLRSLRIRPVITAHPTESKRVTVLEKYRKIYLLLRELEVARWTEREREEILADIR
                     DQIELVWMTGELHLEKPTVEHEVLRGLYFFDETLYERAPEMLELLDGALQRHYPQENF
                     DLPPFFQFGSWIGGDRDGNPHVTTTVTGWALRQNATAALRYYHGRIVDLARALSITER
                     ALPVPERFKGELAHVLKMSGEAESIAHRNPGEPYRQFLTCVLRKLDATMTRAEGSELE
                     LGAPHYGNADELIHELMQVERALAEANSPSLAADLVRPVRRAVELFRFSTVRLDLREN
                     TTRTTETLEALWWASAGHSDGEPPELGSREWRTWLLRALARPLTTEHIIGGLPSEARE
                     LIDMFAQAAEVRRGLDRDAFGSFILSMTRSVDDILGAYLLAKEGGLFLDAAGTEICAM
                     PIVPLFETIDDLRAAPAIMREALAIPLIRRSTHWQGGLQEVMIGYSDSNKDGGFVASN
                     WELTKAQTNLTRVGEEAGTAIAFFHGRGGSVSRGGAPTGRAIAAQPAGSIRGRFRVTE
                     QGEVVSHKYANKGTSLYQMELLASSVLEHALKSEREAALRPSAEFDDALEALAGASRA
                     AYTGLITHPDLVTYFQAASPLEEISLLNIGSRPARRFGASSLKDLRAIPWVFAWAQNR
                     HIITGWYGVGSALQTFMEVRGAQGEALLAKMFADSRLFRLIIDEVEKVLALVDMEIAK
                     DYAGLVDDEGVRATVLAMIEKELALTTEMVLKVSGSAFPAERFPQFRASLAERLPTVN
                     QVNREQVELLRRFRASENEHDREAFKSALLLSINTVAAGLGATG"
     gene            2548..3519
                     /locus_tag="AC16Y7_01910"
     CDS             2548..3519
                     /locus_tag="AC16Y7_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR5708164.1"
                     /translation="MSFTLIQQATPRLHRSELAVPGSNPSLFEKAATSAADVIFLDLE
                     DAVAPDDKEQARKNIIQGLNDIDWGNKTMMIRINGLDTHFAYRDVIDVLENCPRLDMI
                     LIPKVGVPEDVYAIDMLITQVEQYKKYEKKLGLELLIETALGMANVEAIAQASPRVEA
                     MSFGVADYAASTRARTVGIGGPHPDHGVLTDADESGKRDYHWGDPWHYALSRMMVACR
                     AYGLRPIDGPFGDFSDPDGYKAAAKRGAVLGFEGKWAIHPSQIPLANEVFSPSEAEVT
                     KAQRIVEAMEQAAKEGKGAVSLDGRLIDIASIRQAQALLDKAKQITG"
     gene            complement(3624..3800)
                     /locus_tag="AC16Y7_01915"
     CDS             complement(3624..3800)
                     /locus_tag="AC16Y7_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708165.1"
                     /translation="MSVLSLLTSVVLAGLVYGLSFLPNATLTQADDEIGVGLNGDEPA
                     TETKDADTKHTARA"
     gene            complement(3945..4742)
                     /locus_tag="AC16Y7_01920"
     CDS             complement(3945..4742)
                     /locus_tag="AC16Y7_01920"
                     /inference="COORDINATES: protein motif:HMM:NF025389.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin-like domain"
                     /protein_id="HIR5708166.1"
                     /translation="MNGFPVFALITGIVATLVGLSLQFIGRGLFSRHKRDDSKELAGV
                     VGLRVSAIFGIAVGLIFASSASHLMDAKRNLQEEARLIGTLKFLAAEAPTLDNRGKIQ
                     QNLDDFATRSVKELSGPGGPSEARRVTSHLLFEICRGMALDDSDNAQIRWTKVEFQRS
                     CSKLIDLRGRKIIGSRESLVTPPFWVFFIVCFCFLAFLFGVFEPRPLNIVFACLFYFA
                     TGVTGVIIYASSDPYREPGRISTAPLEVVLPKKVAPPPAEVPAGAPN"
BASE COUNT          931 a         1600 c         1619 g          891 t
ORIGIN      
        1 tccatcgccg aacagaacgc cgccatgcgc cgccgccggc atatcgagca gacgcgcggg
       61 cgcgacaagc tccgcggcac gtttgaccat gtgctggccg aagctgccgc ggacggcatc
      121 gcgccgaaag aggtcgatga cctgctgcgc tcgttgcgca tccgccccgt catcacggcg
      181 catccgacgg agtcgaagcg cgtcacggtc ctcgagaagt accgcaagat ctatcttttg
      241 ctgcgcgagc tcgaggtcgc gcgctggacg gagcgggagc gggaagagat cctcgccgac
      301 atccgcgatc agatcgaact cgtgtggatg accggcgagc ttcatctgga gaagccaacc
      361 gtcgagcacg aggtcttgcg cggcctctac ttcttcgacg agacgctgta cgagcgggcg
      421 ccggagatgc tggagctgct ggacggcgca ctccagcgcc attacccgca agaaaatttc
      481 gacttaccgc ctttcttcca gttcggttcg tggattggcg gcgaccgtga cggcaatccg
      541 catgtcacga ccaccgtcac gggatgggcc ttgcggcaga atgcgacggc ggcgttgcgc
      601 tattatcatg ggcgcatcgt cgacctggcg cgcgcgctgt ccatcaccga gcgcgcgctt
      661 cccgtgccgg agagattcaa aggcgagctt gctcatgtct tgaagatgag cggcgaagcc
      721 gagagcattg ctcaccgcaa tcccggggag ccctaccgtc agtttttgac ctgcgttttg
      781 cgcaagctcg atgcaacgat gacgcgcgcc gaaggcagcg agctggaact tggcgcgccg
      841 cattacggca acgccgacga gctgattcac gaattaatgc aggtggagcg ggcgcttgct
      901 gaggcgaata gcccttcgct cgccgccgac ttggtgcggc ccgtgcgccg ggccgtcgag
      961 ctgttccgct tttccacggt gcggctcgat ctgcgcgaga acaccacgcg cacgactgag
     1021 actctagagg ctctctggtg ggccagcgcc ggtcactccg atggcgagcc gccggagctc
     1081 ggcagccgcg agtggcgcac ttggctgctc cgcgcactgg cgcggccgct caccaccgag
     1141 cacatcatcg gtggcctgcc gagcgaggcc agggagctga tcgacatgtt cgcccaggcc
     1201 gccgaagtca ggcgcgggct ggaccgcgac gcgttcggct ccttcattct ctccatgacg
     1261 cgctcggtcg acgacattct cggtgcgtat cttctggcca aggaaggcgg cttgttcctc
     1321 gacgcggcgg gcacggagat ttgcgccatg ccgatcgtgc cgctgttcga aaccatcgac
     1381 gaccttcgcg cggcgcccgc catcatgcgc gaggcgctcg ccattccgct gatccggcgt
     1441 tcgacccatt ggcagggcgg cttgcaagaa gtgatgatcg gctattcgga ttccaacaag
     1501 gacggcggct tcgtcgcctc caactgggag ctgaccaagg cgcagaccaa tttgacgcgc
     1561 gtcggcgagg aggcgggcac ggccatcgcc ttcttccatg gccgtggcgg atcggtatcg
     1621 cggggcggtg cgcccacggg ccgtgccatc gccgcgcagc ccgccggttc gatccgcggc
     1681 cgcttccgcg tgaccgagca gggcgaggtg gtgtcccaca aatacgccaa caagggcacg
     1741 tcgctctatc agatggagct gctcgcctcg agcgtgctgg agcatgcctt gaagtccgag
     1801 cgcgaagcgg ccttgcggcc aagcgccgag ttcgacgatg cgctggaggc gttggccggt
     1861 gcatcgcgcg ccgcctatac gggcctcatc acgcatccgg atctcgtcac ctatttccag
     1921 gcggcgagcc cgctggaaga gatctcgcta ctcaatatcg gctcgcggcc cgcgcggcgc
     1981 ttcggcgcca gctcgctgaa ggacttgcgg gcaatcccgt gggtgttcgc ctgggcccag
     2041 aaccggcaca tcatcactgg ctggtatggg gtgggcagcg cccttcagac cttcatggag
     2101 gtccgcgggg ctcaaggcga ggcgctcctg gccaagatgt tcgccgactc ccggctgttc
     2161 cgcctgatca tcgatgaggt ggagaaggtg ctggccctgg tggacatgga aattgctaag
     2221 gattacgcgg gcttagtcga tgacgagggg gtccgggcga ccgtccttgc gatgatcgag
     2281 aaggagcttg cactgaccac cgagatggtg ctgaaagtgt ccggaagcgc cttccccgcg
     2341 gagcgcttcc cccaattccg cgcctctttg gccgagaggc ttcccaccgt caatcaggtg
     2401 aaccgggagc aggtcgagct gctccgccga tttagagcga gcgagaacga gcacgaccgc
     2461 gaagctttca aatccgcctt actgctttcc atcaacaccg ttgccgccgg tctcggcgcc
     2521 acaggttaaa ccaggaggga ctattcaatg agctttaccc tgattcagca ggcgacgccg
     2581 cgcctgcacc gttcggagct ggcggtgccc gggtccaacc cgtcgctgtt cgagaaggcg
     2641 gccaccagcg ccgccgacgt catcttcctc gaccttgagg acgccgtggc gcccgacgac
     2701 aaggagcagg cccgcaaaaa catcatccag ggcctgaacg acatcgactg gggcaacaag
     2761 accatgatga ttcgcatcaa tggtctcgac acccactttg cctatcgcga cgtgatcgac
     2821 gtgctggaga actgcccgcg cctcgacatg atcctgatcc ccaaggtcgg cgtgccggag
     2881 gacgtctatg ccatcgacat gctcatcacg caggtcgagc aatataagaa gtacgagaag
     2941 aagcttggcc tggagctgct catcgagacg gcgcttggca tggccaatgt ggaagccatc
     3001 gcgcaggcga gcccgcgcgt ggaggccatg agcttcggcg ttgccgacta tgcggcgtcc
     3061 acccgcgcgc gcaccgtcgg cattggcggc ccgcacccgg accacggcgt gctgaccgac
     3121 gccgacgaga gcggcaagcg cgactatcac tggggcgatc cttggcacta tgcgctgagc
     3181 cgcatgatgg tagcctgccg cgcctacggc cttcgtccga tcgacggtcc gttcggcgac
     3241 ttctccgatc cggacggcta caaggccgcg gcgaagcgcg gcgccgttct cggctttgag
     3301 ggcaaatggg ccatccaccc ctcgcagatc ccgctcgcca atgaggtgtt ctcgccgtcc
     3361 gaggccgagg tcaccaaggc ccagcgcatc gtcgaggcca tggagcaggc agcaaaagaa
     3421 ggcaagggcg ccgtgtcttt ggacggccgc ctgatcgaca tcgcttcgat ccgccaggcc
     3481 caggcgctgc tggacaaggc caagcagatc actggctagt cagggcaagc agcctgcgct
     3541 gcgatgaaga tcaataggat taagagaaac ggctggggtt cgtcccggcc gttttttgta
     3601 tctcaaatac gtggatcgcg ggttcaggcg cgcgcggtgt gcttggtgtc cgcgtccttg
     3661 gtctcggtgg ccggctcgtc gccgttcaag ccgacgccga tctcgtcgtc ggcctgcgtc
     3721 agcgtggcgt tgggcagaaa actcaggcca tagaccagcc ctgcaagaac cacactggtc
     3781 aaaagcgata acactgacat gccgatactc catccctcta ctgagagaga ctgtgccagg
     3841 ggacctgggc gccgtctcga ccgaaacccg gcaatttctc ggcaagatcg gaaaatgccg
     3901 ccgcatatcc gccgcaagcc ttgaggtttt gtgaagacga gtcttcagtt cggcgctccc
     3961 gccggtacct cggccggcgg cggcgccacc ttcttgggca ggaccacctc caagggcgcc
     4021 gtgctgatac ggcccggctc gcgatacggg tcgctggagg catagatgat cacgccggta
     4081 acgccggtgg cgaaatagaa caggcacgcg aagacgatat tcagcggtcg cggttcgaac
     4141 acgccaaaca aaaaggcgag gaagcagaaa cacacaatga agaagaccca gaagggcggc
     4201 gtgaccagac tctcgcgcga gccgatgatc ttgcggccgc gcaggtcgat cagcttgctg
     4261 cacgatcgct gaaactcgac cttggtccag cggatttggg cgttgtcgct gtcgtccagc
     4321 gccatgcccc ggcaaatctc aaagagcaga tgactggtga cgcgccgcgc ttcggacggg
     4381 ccgcccggcc cggagagctc cttcaccgag cgtgtcgcga agtcgtccag gttctgctgg
     4441 atctttccgc ggttatcgag cgtcggcgcc tcggcggcga ggaacttcaa ggtgccgatc
     4501 aggcgcgcct cttcttgcag gttgcgcttg gcgtccatca ggtgggaggc ggaggaggcg
     4561 aagatcagac cgacggcaat accgaaaatg gccgagacgc gtaggccaac gacgccggcc
     4621 agctctttgg agtcgtcgcg cttgtggcgc gagaacaggc cccggccgat gaactgcagg
     4681 ctgaggccga caagcgtggc gacgatgccg gtgatcagcg cgaagacggg gaatccgttc
     4741 atctggcggg cgcagctctc tcgcgctcac gcaccgcgcc ggtcgtgtaa ctcatttccc
     4801 tgctccctca aagtgttacg ccttttcagt cttagcccca aatcaaaacg accgcacgcc
     4861 gttcgctttg atatgaatta aggtccgctt ctggcactta gccgacatta gacgcgagat
     4921 ttatccccgc cgctcttttg gtgcgctcta gggggaggga accgaagccc gcgcataaaa
     4981 gcggcggctc cgaggagccg ccgcttcgat tgctcgtttg aagtgcgctg ccgcttagtc
     5041 g
//
LOCUS       DBNHVU010000050        13314 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_835619, whole genome shotgun sequence.
ACCESSION   DBNHVU010000050 DBNHVU010000000
VERSION     DBNHVU010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 13314)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13314)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13314
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_835619"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..1067)
                     /locus_tag="AC16Y7_01925"
     CDS             complement(228..1067)
                     /locus_tag="AC16Y7_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR5708167.1"
                     /translation="MSGEDTPVTAEKPQGPGASDIVSDWEQEAERIEDGDQLRVDVEG
                     FEGPLDLLLTMARTQKVDLSKISVLALAQQYLDFIGEARRLRLEIAADYLVMAAWLAF
                     LKSKLLLPAEPSEEGEPTGEELAALLAFRLKRLDAMRDAAAQLMTRKRLGRDVFGRGL
                     PEPIRVTKTSVYEANVYDLLKAYSQQRQRTATHTLHMEKRSVWSLKEARDELERLLGV
                     ACEWAPLDQLLLEFLVDPEVRRTALASSFTASLEMTREGTLEIRQSKSFGPLLVRRRG
                     EAG"
     gene            complement(1064..2113)
                     /gene="nagZ"
                     /locus_tag="AC16Y7_01930"
     CDS             complement(1064..2113)
                     /gene="nagZ"
                     /locus_tag="AC16Y7_01930"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HIR5708168.1"
                     /translation="MTFKAFISGCASTALTPAETELFGRERPCGLILFARNVQSPGQV
                     QALVADFKAAVGSDEVLILIDQEGGRVQRLRPPHWRAMPPARVFGDLYASDPEAAKRA
                     AFAGARLIAAELTDLGINVNCTPCIDVPQEGAHDIIGDRAFSTDPEVVSALGRAVMDG
                     TLAGGVLPVIKHVPGHGRALADSHKSLPRIDASKEELEQVDFKPFRALHDAPLAMTAH
                     VLLPVCDECHPATLSPAIMGKVIRDQMGLTGLIMSDDIGMEALEGSYADRTRAVIAGG
                     CDVVLHCSGKFAEMTDVAGAAPLLEGKPAERFEAARAALHDPEPFDEAEALALVTEAA
                     GTRVASLGPDPTVQA"
     gene            complement(2161..4011)
                     /locus_tag="AC16Y7_01935"
     CDS             complement(2161..4011)
                     /locus_tag="AC16Y7_01935"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR5708169.1"
                     /translation="MVQGAGRPGQPNIFGAGGGPKFAQMPTYQPVTRAPGEAPQADPQ
                     APYPPQTARPQQPAPAQAQQPAPVQPTAAQPVPAQPAPQAMPPQPAAYQPQQPQPQAF
                     GGQPAAQPQAYTAPSPAAAPSGQELPDLSDADPNFPDSIFGDFKSPEASPPEADFAAP
                     NPAADAGLDAAFPEPVDAGFPEASFPETPAAAGADPALGGGEMAYGASPYDPAAQGAA
                     QAPMQQHPGYPPQDYPQQHPAPQAPQAAPGEPGRALQALAPAYDEPPQIPLGGQQGMA
                     PPPQDYAEAGSDADFMDESQLAAAPAGKGSKLKSSLKGRSAVMVASALLGAIALGGAL
                     AYAYKQSGGSGEAPMITADATPVKEAPDQPGGKEFPHKNKLIYDRLTNGDQPGSERLV
                     PRQEDVAVPALPPATATHSLPTPVATTDLANQAQAAADGARKVNTFVVRPDGSVEAAA
                     AAAGAVTGAAGAATQTAQDAAGQAAQMAQNATGAATQAANGAAAQAAGQAANMMPVPA
                     PQAAPQQVATANPAPAAAPAAPAADSKYVVQLGSSKSQTDALANFADAQQKYPSLLSS
                     HQPVVRKTDLGAKGTWYRLQVGPLADKSAAYKLCGQLKAKGHADCLVMAQ"
     gene            complement(4197..5954)
                     /gene="argS"
                     /locus_tag="AC16Y7_01940"
     CDS             complement(4197..5954)
                     /gene="argS"
                     /locus_tag="AC16Y7_01940"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR5708170.1"
                     /translation="MDVFTGFHGHVLAAVQQLKHDGVVPQDASVDGISLETPKDSGHG
                     DLATNAAMVLAKPAKLAPRALAERIVPLIASDPYVEKVEIAGPGFINLTLARAFWPSV
                     LRMVLEQGDAFGRSAIGKGQAVNVEYVSANPTGPMHVGHCRGAVFGDALANLLDYASY
                     DVTREYYVNDAGAQVDVLAQSAFLRYREALGEDIGAIPEGLYPGDYLKPVGEQLAGNH
                     GRDLLDMPEAEWLPLVKAAALKAMLAMIADDLSQLGIKHDVFFSERSLTDGKDEVAET
                     IDDLRAKGLIYEGRLPPPKGKVDEAWEDREQLLFRASDFGDDQDRPLVKSDGSYTYFA
                     SDIAYHYDKFRRGFHSMIDVWGADHAGHIKRLRAALSALTVGKADLDVKVCQLVRLFR
                     SGEPVKMSKRAGTFVTLRDVVEEVGAGPVRFMMLYRKNDAPLDFDFAKVTEQSRDNPV
                     FYVQYAHARASSVLRNVAETFADLSVAEDVLVQADLNQLNDDGEIALIRRLAQFPRIV
                     EGAAQAHEPHRLSFYLYDLAGDFHGLWNRGKDLPQLRFIYESDRELTRARVALVAATK
                     HVLALGLGILGVHAMNELH"
     gene            complement(6035..7249)
                     /locus_tag="AC16Y7_01945"
     CDS             complement(6035..7249)
                     /locus_tag="AC16Y7_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HIR5708171.1"
                     /translation="MSQPAKPEPDVSAPTGRGPLAPYAVDARVSRGRLIAEPPSPTRT
                     AYARDRDRILHSSAFRRLNYKTQVFVYHEGDHYRSRLTHSLEVAQIARSIARSLRVDE
                     DLTETVALAHDLGHSPFGHAGERALDAAMTDAGGFDHNAQSLRVVTRFERKYGAFDGL
                     NLTWETLEGLVKHNGPLEGHETPVPEPVLDYCATHDLELDTYASLEAQIAALSDDIAY
                     NAHDLEDGYRANCFTFAELAEVPMTASLLAEVDAAYPDLDDLRRLYEVTRRLITAMVN
                     DAIAEARARLATLRPESADDVRQAGRTLIAFSDEMLGRLDDLRKFLFARVYRHARVVR
                     IMDDAETVIADLFERYTETVDALPPEWRAEAPPLGTRQYARYVADFIAGMTDRYALVE
                     HRRLFDATPDLR"
     gene            7323..7655
                     /gene="erpA"
                     /locus_tag="AC16Y7_01950"
     CDS             7323..7655
                     /gene="erpA"
                     /locus_tag="AC16Y7_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HIR5708172.1"
                     /translation="MSDPSITISTRAAKRIVELVSEDDAARLFRVSVEGGGCSGFQYR
                     FELVKDTAPDDIVIERDGATVAIDPVSAGFVKGSEIDFVDELIGAQFKIHNPNATAAC
                     GCGTSFSI"
     gene            7730..8509
                     /gene="xth"
                     /locus_tag="AC16Y7_01955"
     CDS             7730..8509
                     /gene="xth"
                     /locus_tag="AC16Y7_01955"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR5708173.1"
                     /translation="MKIASWNINGVKARIETAVTWLKAAAPDVVCLQEIKSVDEAFPA
                     ERFEELGYNVATHGQKGFNGVALLSKLPFDDVSPGLAGDPDDTQARYLEAVISVPSGV
                     VRVVNIYLPNGNGSPEKFAYKLAWMRRLQARVMTLLTYEEPFVVAGDFNVIPAPEDVY
                     NPEAWMDDALFRPETRARFRALVNLGLTEAFRACHDEPHQFTFWDYQAGAWQKNHGLR
                     IDHFLLSPQAADRLQSCEVDPEPRGWEKPSDHVPIVVELSL"
     gene            complement(8523..9482)
                     /locus_tag="AC16Y7_01960"
     CDS             complement(8523..9482)
                     /locus_tag="AC16Y7_01960"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5708174.1"
                     /translation="MPMFSASTIGSALAAVIVLTPAGIGAADEPPLYESATQAYRQGV
                     SAMKSGEMTAAVAALEYAAGRGVLGAQLKLARLYAAGRDVPKDDTKAFFHFQKIADQY
                     ADIPPSSPIAKYVGESFAALGKYYVSGIEAMPLHPDPVYAAGLFRHASAYFGDAEAQY
                     QLGCLYRTGAGVEKNSTIAANWLAMAARKQHPGAQALLGEMLWRGDGVSQRPARGLAL
                     LMLAEENAKAKGGEPEQIRQLYKETLAEADSGTRKRAMALLPQLGGPNRATARAMTKP
                     VKSDPKLTASKEPQLGPVDLTPPAAMGLSVGFGASGDGLGVPQ"
     gene            complement(9469..11193)
                     /gene="ilvD"
                     /locus_tag="AC16Y7_01965"
     CDS             complement(9469..11193)
                     /gene="ilvD"
                     /locus_tag="AC16Y7_01965"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR5708175.1"
                     /translation="MDAKTFDKSRLPSRHVTEGPSRAPHRSYYYAMGLTRGQIHQPFV
                     GVATCWNEAAPCNIALMRQAQAVKQGVAAKDGTPREFCTITVTDGIAMGHQGMKSSLP
                     SREVIADSVELTMRGHCYDALVGLAGCDKSLPGMMMAMCRLNVPSIFIYGGSILPGTY
                     KGRPVTVQDLFEAVGQYSVGKMSPEDLEELEHVACPSAGACGAQFTANTMATVSEAIG
                     LALPYSAGAPTPYEIRDQFCMMAGEKIMDLIALNIRPRDIISRKSLENAAAVVAASGG
                     STNAALHLPAIAHECGIDFDLFDVAEIFKKTPYIADLKPGGRYVAKDMFEAGGVPLLM
                     KTLLDHGYMHGDCLTVTGRSIAENLSKVAWNPDQDVVRSADNPLTPTGGVVGLQGTLA
                     PDGAIVKVAGLNNLKFRGPARCFDSEEEAFAAVSAKKYDEGDVIIIRYEGPKGGPGMR
                     EMLSTTAALYGQGMGDKVALITDGRFSGATRGFCVGHVGPEAAVCGPIALVRDGDMIV
                     LDAEDGVIDLEVSEDELAARAKDWTAPPTSFQSGYLWKYANEVGAARTGAVTHPGGAA
                     EGSCYADV"
     gene            complement(11460..>13314)
                     /locus_tag="AC16Y7_01970"
     CDS             complement(11460..>13314)
                     /locus_tag="AC16Y7_01970"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR5708176.1"
                     /translation="KRHGLPMINVLSKDARLQLQANSEFTDGVEMTPQLHATILELNG
                     VFRFAARKMIVDRLEDEGLLEKTEPHVLAVPHGDRSGAVIEPYLTDQWYVDAATLAKP
                     AIDAVRRGRTTFVPKNWESTYFHWMENIQPWCISRQLWWGHQIPAWYGPDGEIFVAET
                     EADAKAAAKAHYGKDATLKRDEDVLDTWFSSALWPFSTLGWPDKTPELARYYPTDVLV
                     TGFDIIFFWIARMMMMGLHFMDEVPFRDIYIHALVRDEKGQKMSKSKGNVIDPLELID
                     SYGADALRFTLAAMAAQGRDIKLSGNRVEGYRNFATKLWNASRFAMMNDCVPQADFDP
                     TSAQVTVNRWIAGEVERTTAAVTEALEAFRFNDAAAAIYHFIWHIYCDWYLELIKPIL
                     MGEDEVAKAETRAMAAYVLDRALQLLHPFMPFVTEELWEKRAPEGTRRETLLMLTEWP
                     THLGLQNAKADAEMDWVIRFVTEVRSVRAEMNVPAGAKVPLVIAGATDKTKAQVAAHE
                     ETLKRLARIKTIDFTDAPPSGSVQMVLDEATLAMPLEGVIDIGAESERLKREIDKMGS
                     EIAQLDARLANEKFVSRAPEHVVEEQRVRKSEAEATAAKLAQALKRLEAAL"
BASE COUNT         2269 a         4293 c         4292 g         2460 t
ORIGIN      
        1 tcctccgaca gcttcgatat gtcggagagc accgcggtca ggttcggctt ggactcatct
       61 gccttggact catctgcctc gcccctatct cgagcaagct tcgatggcaa aattttgggt
      121 ttcggcatga tatgcccctc gcgtatcggc cagtaacgcg cctgccaggt accggtggcc
      181 tcattcctcc actgactgca ctcgattcaa cgcaaatcca actcaactca acccgcttcg
      241 ccgcgacggc gaaccaggag cggtccaaac gacttcgact ggcggatctc aagggtgcct
      301 tcgcgtgtca tctccaagct cgcggtgaag ctcgacgcca gcgcggtgcg ccggacctcc
      361 gggtccacca agaactccag aagcagctga tcgagcggcg cccactcgca ggcgacaccg
      421 aggagccgct ccagttcgtc gcgcgcctcc ttaagcgacc atacggagcg cttctccatg
      481 tgcagcgtgt gcgtcgccgt gcgctgccgc tgctgggaat acgctttcaa gagatcgtag
      541 acattcgcct cataaacgct ggtcttggtg acgcggatgg gttccggcag cccccgcccg
      601 aaaacatcac ggccaaggcg cttgcgcgtc atcagctgag ctgccgcatc gcgcatggca
      661 tcaaggcgct tcagcctaaa ggccaagagc gcggcaagct cttcaccggt cggctcgccc
      721 tcctccgacg gttcagccgg aagcaggagc ttcgacttca agaaggccag ccacgcggcc
      781 atgacgagat aatcggcggc gatctccaag cgcaggcgtc gagcctctcc gatgaagtcg
      841 agatattgct gcgccagcgc cagcacggaa atcttggaga ggtcgacctt ctgggttcgc
      901 gccatcgtca aaaggagatc cagcgggcct tcgaacccct caacgtcgac ccgaagctga
      961 tcgccgtcct cgatgcgctc ggcctcctgt tcccagtcgg aaacaatgtc ggaagcgcca
     1021 gggccttgcg gcttctctgc tgtcactggt gtgtcttcgc cgctcatgcc tgcaccgtcg
     1081 ggtctggacc aagactcgcc acgcgggttc cggcagcctc ggtcaccagc gccaaagctt
     1141 ctgcctcgtc gaaaggctca gggtcgtgca gagccgccct cgccgcctcg aagcgttccg
     1201 caggcttccc ttccaaaagg ggtgctgcgc cggcgacgtc ggtcatctcc gcgaacttgc
     1261 cggagcagtg gagcaccaca tcgcagccgc ccgcgatcac cgcgcgcgtg cggtccgcgt
     1321 acgagccctc aagggcctcc atgccgatgt cgtcactcat gatgagtccg gtcaatccca
     1381 tctgatctcg aatcactttc cccattatag cgggggagag ggtcgcaggg tggcactcgt
     1441 cgcaaacagg cagcaaaacg tgcgcggtca tcgccagggg cgcatcgtgc agcgctcgaa
     1501 acggcttgaa atccacctgc tcgagctctt ccttggacgc atcgatccgc ggcagcgatt
     1561 tgtggctatc cgctagggcg cgaccgtggc caggcacgtg cttgatgacc ggcagaacgc
     1621 cgcctgcgag cgtgccgtcc ataaccgccc gccccaaggc gctcaccact tcaggatctg
     1681 tggagaacgc ccggtcgccg atgatgtcgt gggcgccttc ttggggcacg tcgatgcacg
     1741 gggtgcagtt cacgttgatg ccgaggtcgg tcagttcagc cgcgatcagc ctggcgcctg
     1801 cgaacgcggc acgcttggcc gcttccggat cggacgcata gaggtcccca aatacccggg
     1861 ccggcggcat ggcccgccaa tgcggcggac gaagccgctg cacgcggcca ccttcttggt
     1921 cgatgaggat cagcacctcg tcgctgccga ccgcagcctt gaagtcggcg acaagcgctt
     1981 ggacctgtcc ggggctctga acgttcctgg cgaacaggat gaggccgcaa ggccgctcgc
     2041 gtccgaagag ctcagtctcc gcaggcgtca gcgcggtgct cgcgcatccc gagatgaaag
     2101 ctttgaaagt cacgttgcgg ggctagcccc gccgcgcgcg gcggtcaagc gttccgtggc
     2161 ctattgcgcc attaccagac agtcggcgtg ccctttcgcc ttcagctgac cgcaaagctt
     2221 gtaggccgcg gacttgtcgg ccagaggccc gacctgaagc cgataccaag tgcccttggc
     2281 gccgagatcg gtcttgcgaa cgacgggctg atggctgctc aacaggctcg gatatttctg
     2341 ctgggcatcg gcgaagttcg ccagcgcatc ggtctggctc ttgctggagc cgagctgcac
     2401 gacatatttg gagtcggctg cgggagccgc tggtgctgcg gctggcgccg ggttggcagt
     2461 ggccacctgc tgcggcgcag cctgtggcgc cggcaccggc atcatgttgg ccgcctgacc
     2521 cgccgcttgg gcggcagcgc cgttcgcggc ctgggttgct gctccggtgg cgttctgagc
     2581 catctgggcc gcctggccgg cagcgtcctg ggcggtttgt gtcgccgcac cggccgcgcc
     2641 tgtaacggcg cctgcagccg ctgcggcagc ttcgacggag ccgtcaggac ggacgacgaa
     2701 agtattgacc ttgcgcgcac catccgccgc ggcctgggcc tggttggcga gatcggtggt
     2761 ggcaacgggc gtcggcaaac tgtgggtcgc ggttgccggc ggcagggccg gtaccgccac
     2821 gtcttcctgg cggggcacga gacgctcgga cccgggttga tcgccattgg tcagacgatc
     2881 gtagatcagt ttgtttttgt gaggaaattc cttgccgccc ggctggtcgg gggcttcctt
     2941 caccggcgtc gcgtcggccg tgatcatcgg cgcctcgccg ctcccgccgc tctgcttgta
     3001 ggcgtaggcc agagcaccgc cgagcgcgat cgctccaagc agtgcgcttg cgaccatgac
     3061 ggcgctgcgg cctttcagcg aactcttcaa cttggagccc ttacccgccg gcgcggcagc
     3121 aagctggctc tcatccataa aatcggcatc gctcccggcc tcggcgtagt cctgcggcgg
     3181 gggcgccatg ccctgctggc cacccagcgg gatttgcggg ggctcgtcgt aggcgggggc
     3241 aagggcctga agtgcacgtc caggctcgcc aggcgcagcc tgaggggctt gcggcgctgg
     3301 gtgttgctgc gggtagtctt ggggcggata tcccggatgc tgctgcatgg gcgcctgagc
     3361 agcaccttgc gcggccgggt cgtagggcga cgcgccatat gccatctcgc cgccgccaag
     3421 cgctgggtcc gcgccggccg ccgcaggtgt ctccgggaag ctcgcctccg ggaaaccggc
     3481 atcaaccggc tcggggaagg ccgcatcgag gccagcgtcc gcagccgggt tcggcgcggc
     3541 gaaatcggcc tccggggggc tcgcttcggg gctcttgaag tctccgaaga tcgagtcggg
     3601 aaaattcgga tcggcgtcgc tcaggtccgg cagctcttgg ccgctcggcg ccgcggcggg
     3661 gctgggcgcc gtgtaggcct gcggctgggc cgccggctgt ccaccgaagg cttgcggttg
     3721 aggctgttgg ggctggtagg ccgcaggctg gggcggcatg gcctgaggtg caggctgggc
     3781 cggcacaggc tgagcggctg tgggctgaac gggagcgggc tgctgggctt gcgccggagc
     3841 aggctgctgg ggccgcgccg tctggggcgg atagggcgcc tgcggatcgg cttggggcgc
     3901 ctctccgggc gcgcgcgtca ccggctgata ggtcggcatc tgggcgaatt tcggaccacc
     3961 gccggcaccg aaaatgtttg gctgacctgg ccgccccgct ccttgaacca tgaaagatac
     4021 ctcactcttt tcgatgttgg ccgtgctcgc gtcccaggat tacccggcgc aggcggcggc
     4081 gtgcctgttg atcaaatctg atgcaaattt tgcggatcgg ccagcccagc ggccgaaccc
     4141 actctcgcgc gtggacatgg cttaagtccc tcgaactacc ccctgcccca ggccggctaa
     4201 tgcaactcgt tcatggcatg aacgccgagt attcccaaac ccaacgccaa gacatgcttt
     4261 gtggcggcca cgagcgcaac acgagcacgt gttaactctc tgtcactctc gtaaataaaa
     4321 cgtaattgtg gcaagtcttt gcctctgttc caaagaccat ggaagtctcc agcaagatcg
     4381 taaagataga aactgagccg atgtggctca tgtgcttgcg ctgcaccctc aacaatacga
     4441 ggaaattgag ccagacgacg gatcaaggcg atctccccgt cgtcattcaa ctgatttagg
     4501 tcggcctgca ccaacacatc ctccgctaca ctgaggtccg caaatgtctc agcaacatta
     4561 cgcagaaccg aactcgcccg ggcgtgggcg tactgaacat agaagaccgg attgtcccgc
     4621 gactgctcgg tgaccttggc gaagtcgaaa tcaagcggcg cgtcgttctt ccggtacagc
     4681 atcatgaagc gaacgggccc tgcacccact tcctccacga cgtcccggag cgttacgaat
     4741 gtcccggcac gcttggacat tttgacgggc tcaccagacc gaaacaagcg gaccagctgg
     4801 cataccttaa cgtcgagatc ggccttgccg accgtcagcg cggacagggc ggcgcgcagg
     4861 cgcttgatat ggcccgcatg gtcggcgccc cagacgtcga tcatggagtg aaaaccgcgt
     4921 ctgaacttgt cgtagtgata ggcgatatcg gaggcaaaat acgtgtatga gccatccgac
     4981 ttgacgagcg gccggtcctg gtcgtcgccg aagtcgctgg cgcggaacag gagctgctcg
     5041 cggtcctccc aggcctcgtc caccttgccc ttgggcggag gcaaacggcc ctcgtagatc
     5101 aggcctttgg ctctcaggtc gtcgatggtc tcggcgacct cgtccttgcc gtcggtgagc
     5161 gagcgttccg agaagaagac gtcgtgcttg ataccgagct ggctcagatc gtccgcgatc
     5221 atggccagca tggccttcag cgcggcggcc ttcaccagcg gcagccactc ggcctccggc
     5281 atgtcgagca gatcccggcc gtgattgccg gcaagctgct cgccgaccgg cttcaggtag
     5341 tcgccgggat agagcccctc tgggatcgcg ccgatgtcct cgccgagcgc ctcgcggtag
     5401 cggagaaacg cggactgcgc gagcacgtcg acctgcgcgc cggcatcgtt gacatagtac
     5461 tcacgggtga cgtcgtagct ggcgtagtcg aggagattag ccagcgcatc gccgaacacg
     5521 gcgccccggc agtgacccac atgcatggga cccgtcgggt tggcggacac atactccaca
     5581 ttcacggctt gccccttgcc gatggcgctt cggccaaacg catcaccctg ctccagcacc
     5641 atacgcaaca cgctcggcca aaacgcacgg gcgagtgtca ggttgatgaa tccgggtccg
     5701 gcgatctcca ccttctccac atacggatcg gaggcgatca gaggaacgat ccgttcggcg
     5761 agcgcgcggg gcgcgagctt cgcgggcttg gccagcacca tggcggcgtt ggtggcgagg
     5821 tccccatggc cggagtcctt gggcgtctcg agcgaaatgc cgtcgacgct cgcgtcctgc
     5881 ggcacgacgc cgtcatgctt gagctgctgg acggccgcca gaacgtggcc gtgaaaccct
     5941 gtgaaaacgt ccatggatgc tccgggaaac tttttcgcgc atgatcttat cggaaaaccg
     6001 gtgcccactt ctccggatca tgcgtggcgc gcgactatcg caaatccggg gtggcgtcaa
     6061 acagacgccg gtgctccacc agcgcatagc ggtcggtcat accggcgatg aagtccgcca
     6121 catagcgggc gtattggcgc gtccccagcg gcggcgcctc ggcgcgccat tccggcggca
     6181 gagcatcgac cgtctccgtg tagcgctcga acaggtccgc gatgacggtc tccgcatcgt
     6241 ccatgatgcg cacgacccgc gcgtggcggt acacccgggc gaagagaaac ttccgcaagt
     6301 cgtcgaggcg gcccagcatc tcatcagaga acgcgatgag cgtacgtccg gcctggcgca
     6361 cgtcgtcggc gctttcggga cggagcgtgg caagccgcgc gcgcgcttcg gcgatggcat
     6421 cgttgaccat cgcggtgatc agccgccgtg tcacctcgta gaggcgcctt aggtcgtcca
     6481 ggtccggata ggccgcgtcg acttcggcaa gaaggctcgc agtcatcggc acttcggcaa
     6541 gctcggcgaa ggtgaagcag ttcgcgcgat agccatcttc gagatcgtgc gcattgtagg
     6601 cgatgtcgtc ggagagcgcg gcgatttgcg cttcgagcga tgcgtaggta tcgagttcca
     6661 ggtcatgggt cgcgcaatag tccagcacgg gctccgggac cggcgtctcg tgcccctcaa
     6721 gcggcccgtt gtgcttcacc aaaccttcca gcgtctccca ggtgagattg agtccgtcga
     6781 aggcgccgta tttcctttcg aacctcgtga cgacacgcaa ggactgagcg ttgtgatcga
     6841 agccgcctgc gtccgtcatt gcggcatcga gcgcgcgctc gcccgcatgg ccgaagggcg
     6901 aatggccgag atcgtgcgcg agcgcgacgg tctcggtgag atcttcgtcg acccgcagcg
     6961 accgcgcgat ggagcgggcg atctgcgcca cttcgagact gtgggtgaga cgcgaccggt
     7021 aatggtcgcc ctcgtgatag acgaagacct gggtcttgta gttcagccgg cggaaggcgc
     7081 tcgaatgcag aatccggtcg cggtcgcgcg cataggccgt gcgcgtcggg ctcggcggct
     7141 cggcgatcaa gcgcccgcgg ctcacccgcg cgtccacggc atagggcgca agggggcccc
     7201 tccccgttgg ggcgctgacg tctggctcag gctttgcggg ctggctgatt gacaactccc
     7261 ttggccattc tcatctttag tgtatcaaac acttggctga acagaacttg gctataccga
     7321 agatgagcga cccgtccatc accataagca cccgcgccgc caagcgcatc gtcgagctcg
     7381 tcagcgaaga cgacgccgcg cgcctgtttc gcgtcagcgt cgagggtggc ggctgctcgg
     7441 gatttcagta ccgctttgag ctggtgaagg acaccgcgcc cgacgatatc gttatcgagc
     7501 gcgacggcgc cacggtcgcc atcgatcccg tctccgcagg tttcgtgaag ggatcggaaa
     7561 tcgacttcgt cgacgagctg atcggtgctc agttcaagat ccacaatccg aacgccaccg
     7621 cggcttgcgg ctgtggcacc agcttctcga tctagcgcgc gatttggact atgtcccgtc
     7681 cttgccgggt cttcgcctcg gcgtgacgac ttgctatgca tgagtcccca tgaagatcgc
     7741 ctcctggaac atcaacggcg tcaaggcgcg catcgagacc gcggtgacgt ggctgaaagc
     7801 cgccgccccc gatgtcgtct gcctgcaaga gatcaaatcc gtggacgagg cttttccggc
     7861 cgagaggttc gaggagctcg gctataacgt cgcgacccac ggccagaagg gcttcaacgg
     7921 cgtggccttg ctgtccaagc tgcccttcga cgatgtgagc ccggggctcg ccggcgatcc
     7981 agacgacacc caggcgcgct atctggaagc cgtgatctcg gtgccgtccg gcgtcgtgcg
     8041 ggtggtgaac atctatctac cgaacggcaa tggctcgcca gagaagtttg cttacaagct
     8101 ggcgtggatg cgccgcctgc aagcgcgggt gatgacgctt ctcacttacg aggagccgtt
     8161 cgtcgtcgcg ggcgacttca acgtcatccc cgcgccggag gatgtctaca atccggaagc
     8221 ctggatggac gatgcgctgt tccgccccga gacgcgggcg cggttccggg cgctggtcaa
     8281 tctgggcctg accgaagcgt tccgagcctg ccacgacgag ccccaccaat tcaccttttg
     8341 ggactatcag gccggcgcgt ggcagaagaa ccacggactg cgcatcgacc acttcttgct
     8401 gtcaccgcaa gccgccgacc gcttgcagtc atgcgaggtc gatcccgagc cgagaggctg
     8461 ggagaagccg tccgatcacg tgccgattgt tgtggagctt tcgctttaag ccgcctgcgc
     8521 gactattgcg gaacaccgag cccgtcaccc gaggccccga agccaacgga caggcccatg
     8581 gcagccggcg gcgtcaggtc aaccggacca agctgcggct ccttggaggc ggtcagcttc
     8641 gggtcggatt tgacgggttt ggtcatagcg cgggccgtgg cgcgattggg accgccaagt
     8701 tgcggcagca aggccatcgc tcgcttgcgc gtcccgctgt cggcctcggc caaggtctct
     8761 ttgtacagtt gtcggatctg ctccggctcg ccgcccttcg ccttggcgtt ctcctcggca
     8821 agcatcagaa gcgcaagtcc ccgcgccggc cgctggctga cgccatcgcc ccgccacaac
     8881 atctcaccta gaagggcttg cgcgccgggg tgctgcttcc gcgccgccat ggccagccaa
     8941 ttggcggcga tggtcgagtt cttctccacg cccgcgccgg tgcggtagag acagccgagc
     9001 tgatactgcg cctcggcgtc gccgaaatag gcactggcat gccggaacaa gccggcagcg
     9061 taaaccggat cggggtgcag cggcatcgct tcaataccgc ttacgtaata cttgccgagc
     9121 gccgcgaacg actcgccgac atatttggcg atcgggctcg acggcgggat atcggcatat
     9181 tggtcggcga tcttctggaa gtggaagaac gccttcgtgt cgtccttcgg cacgtcacgg
     9241 cctgcggcgt agagacgtgc gagcttgagc tgagccccga gcacgccgcg cccggcggca
     9301 tattcgagcg ccgcaacggc cgctgtcatc tcgccggact tcatcgcgct cacgccctgg
     9361 cgataggctt gcgtcgcgga ctcgtaaagc ggtggctcgt cggccgcgcc gatcccggcg
     9421 ggcgtcagga caatgacggc ggccaacgcg ctgccaatgg tggaggcgct aaacatcggc
     9481 atagcacgac ccctccgcag caccgcccgg gtgggtcact gcgcccgtcc gggccgcccc
     9541 aacctcgttg gcgtatttcc acagatagcc gctttggaac gacgtcggcg gtgccgtcca
     9601 gtccttcgcg cgcgcggcga gttcgtcttc gctgacctca aggtcgatga cgccgtcttc
     9661 ggcatcgagc acgatcatgt cgccgtcgcg caccagcgca atgggaccgc acacggccgc
     9721 ctccggcccc acatggccaa cgcagaaacc gcgcgtggcg cctgaaaagc gtccgtcggt
     9781 gatgagcgca accttgtcgc ccatgccttg gccgtatagc gccgcggtgg tcgacagcat
     9841 ctcccgcatg cccggaccgc ccttcggccc ttcatagcgg atgatgatga catcgccctc
     9901 gtcgtacttt ttggcgctga cggcggcgaa cgcctcttcc tcgctgtcga agcagcgggc
     9961 gggtccgcgg aacttcaagt tgttcaatcc ggccaccttc acgatcgcgc cgtccggcgc
    10021 gagcgtcccc tgcaatccca cgacgccacc ggtgggcgtc agcgggttgt cagcagaacg
    10081 cacaacgtcc tggtccgggt tccacgcaac cttggaaaga ttttccgcga tcgagcggcc
    10141 cgtgaccgtc aggcagtcgc cgtgcatata accgtggtcg agtagcgtct tcatgaggag
    10201 cggcacgcca ccagcttcga acatatcctt ggcgacataa cggccacccg gtttcaggtc
    10261 cgcgatatac ggcgtcttct tgaagatttc cgctacatcg aacagatcga agtcgatacc
    10321 gcattcatgg gcgatggccg gcagatgcag cgccgcgttg gtcgagccgc ccgaggccgc
    10381 gaccacagcg gccgcgttct ccaaggattt gcggctgatg atgtcgcgcg gacggatgtt
    10441 cagcgcgatc aagtccatga tcttctcgcc cgccatcatg caaaactggt cgcggatttc
    10501 gtaaggcgtg ggcgcgccgg ccgagtatgg cagtgcaagg ccgatagcct ccgacaccgt
    10561 cgccatggtg ttcgcggtga actgcgcgcc gcatgcaccg gccgaggggc acgccacgtg
    10621 ctccaactcc tcgaggtcct ccggggacat cttgccgacg gagtactgcc cgacggcttc
    10681 gaacaggtcc tgcaccgtga cgggacggcc cttgtaagtg ccgggcagga tcgagccgcc
    10741 atagatgaag atcgacggga cgttgagccg gcacatggcc atcatcatgc ctggcagaga
    10801 cttgtcgcag ccggcgagcc cgaccaacgc gtcgtagcaa tggccgcgca tggtgagctc
    10861 gacactgtcg gcgatgacct cgcgcgaggg cagcgaggac ttcatgccct ggtggcccat
    10921 ggcgatgccg tcggtgacgg tgatggtgca gaattcgcga ggtgtgccgt ccttggctgc
    10981 aacgccctgc ttgacggcct gggcctggcg catcagggcg atgttgcagg gcgcggcctc
    11041 gttccagcag gtggcgacgc caacgaaggg ctgatgaatt tggccgcgcg tcagccccat
    11101 cgcgtagtag tacgagcgat gtggggcgcg ggacggcccc tccgtcacat gcctgctcgg
    11161 caagcgggat ttatcgaacg tctttgcgtc catgtaccaa tactgggctg gagctgaatg
    11221 agccgccggt cgtccgccgc tctacacccc agccggtcgc tccctggccc tgccccctcg
    11281 cttttgcgct acgcgagtgt ctgatgagcc ataatcctcg aactgcagag gatgtcccaa
    11341 agttttatgg cgcaaacgag gcgcaaatac ctcagtctgt tgcaatttag ctacattttc
    11401 gaggattcag ggcaacggtt gcttggtggg aactttgccg ccgtccggtg aagacgccct
    11461 taaagcgcgg cttcgagccg ctttagcgcc tgcgccagct tggccgcggt ggcttccgct
    11521 tcggacttgc ggacgcgttg ctcctcgacc acatgctcgg gcgcccggga gacaaatttc
    11581 tcgttggcga gcctcgcatc cagctgggcg atctcagacc ccattttgtc gatctcgcgt
    11641 tttaggcgct ccgactccgc gccgatgtcg atcactcctt cgaggggcat ggcgagcgtc
    11701 gcctcgtcca gcaccatctg gaccgagccg gacgggggcg catcggtaaa gtcgatggtc
    11761 ttgatgcggg cgagccgctt gagcgtctcc tcgtgagcgg cgacctgcgc cttggtcttg
    11821 tccgtagcgc cggcgatcac cagcggcacc ttggcaccgg ccggaacgtt catctcggcg
    11881 cgcacggagc gcacctcggt aacgaagcgg atgacccaat ccatctccgc gtccgccttg
    11941 gcattctgga gtcccaaatg agtgggccac tcggtgagca ttagcagcgt ctcgcggcgc
    12001 gtcccttcgg gcgcacgctt ctcccacagc tcctcggtga cgaagggcat gaagggatgc
    12061 aggagctgga gcgcgcggtc gagcacgtac gccgccatgg cgcgggtctc ggccttcgcc
    12121 acctcgtcct cgcccatgag gatcggcttg atcagttcga gataccagtc gcagtagatg
    12181 tgccaaatga agtggtagat ggcggcggcc gcatcgttga agcggaaggc ctcgagtgct
    12241 tcggtcacgg ccgcggtcgt acgctcgacc tcgcccgcga tccagcggtt gacggtgact
    12301 tgcgcgcttg tgggatcgaa atcggcctgg ggcacgcaat cgttcatcat ggcgaagcgc
    12361 gatgcgttcc agagcttcgt cgcgaagttg cggtagcctt cgacgcggtt gccggagagc
    12421 ttgatgtcgc gcccttgcgc ggccatggcg gcgagcgtga agcgcagcgc atcggcgcca
    12481 tagctgtcga tcagctccag gggatcgatg acgttcccct tcgacttcga catcttctgg
    12541 cccttctcgt cgcggaccag cgcgtggatg tagatgtcgc ggaacggcac ctcgtccatg
    12601 aagtgcagcc ccatcatcat catgcgggcg atccagaaga agatgatgtc gaagcccgtg
    12661 acgagcacgt cggttgggta atagcgggca agttccggcg tcttgtccgg ccagccgagg
    12721 gtagagaatg gccacagcgc cgaggagaac caggtgtcga gcacgtcctc gtcgcgcttc
    12781 agcgtcgcgt ccttgccgta atgtgccttg gcggccgctt tggcatccgc ttcagtctca
    12841 gcgacgaata tctcgccgtc gggcccatac caagcaggga tctgatgccc ccaccagagc
    12901 tggcgggaga tgcaccatgg ctggatgttc tccatccagt ggaagtaggt ggactcccaa
    12961 ttcttcggca cgaaggtcgt gcgcccgcgc ctgaccgcgt cgatggcggg cttggccagt
    13021 gtcgcggcgt cgacatacca ctgatcggtg agataaggct cgatgaccgc gccggagcgg
    13081 tcgccatggg gcacggcgag gacgtggggc tcggtctttt cgagcaagcc ctcgtcctcc
    13141 agacgatcga cgatcatctt gcgcgcggcg aagcggaaca cgccgttcaa ttccagaatc
    13201 gttgcatgga gttgcggcgt catctcaacc ccgtccgtga attcggagtt ggcttgtagc
    13261 tggagccgcg cgtccttgct gaggacgttg atcatcggca ggccgtggcg cttg
//
LOCUS       DBNHVU010000051         6613 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1281917, whole genome shotgun sequence.
ACCESSION   DBNHVU010000051 DBNHVU010000000
VERSION     DBNHVU010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6613)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6613)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6613
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1281917"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..365)
                     /locus_tag="AC16Y7_01975"
     CDS             complement(135..365)
                     /locus_tag="AC16Y7_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708177.1"
                     /translation="MMRIGNALPGLLIAALAIATAGPALAKQKQKPQQVTKTTQKQSG
                     GQNCVTTAPPFMRNPRYMNRGFFKKKCYGKKN"
     gene            537..818
                     /locus_tag="AC16Y7_01980"
     CDS             537..818
                     /locus_tag="AC16Y7_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708178.1"
                     /translation="MVRVLFLALGLLIVASAGLQAQTQSLRPTPNACTSGSQSVRVCN
                     NDFQSCNDVCVARALDPSTEIVGCTTACCYKFNVCLRMRGCGPRAINCD"
     gene            complement(829..2718)
                     /locus_tag="AC16Y7_01985"
     CDS             complement(829..2718)
                     /locus_tag="AC16Y7_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708179.1"
                     /translation="MAVAMRKKRAWSTRADDAESQETAAFEASVAAAKDTAAKSGAFL
                     SDAKSDLTDHQRWLRTQSAAVERDRQRHERWLQRQRDRRVASARKERLRLRRQLVRQR
                     AFQAMRHALWAGMLVVSAWYVFALTALRAGIFSVGRLITRGARVAGRRIQAGASWMVA
                     KLGVLAHRLGALVVAGSLFAASKSGTAARTAGRSLGSGLSVASAKTSALAGVTGHAAS
                     RGLAAAGKKTAAVQAAGKKGLANGFEWTMAKTAAVTPTLYVGMAKLGRQAERYARAGV
                     AQAEGMMARAAAKTSKAAALYGPHVGGAEITSRAANDPGSSPHSSVEEPKSAAEAAEV
                     YGPFYEGFWVKGVSPNEPRVAEAAPAPIAPPAEAKNALSWVSSWAHAASARAGASAGQ
                     MRSVAVSSAVQAKAWAAQSQAWVQTRDVDLSRMMIIAGAVLLVCGGLLVGGGLLLRAG
                     AGTAATAAAEEESGVGIALTFQETDRPLSDRAVFTLSGTPAAFRINGLSVGGMNLSDQ
                     PLTGVEAVLKPDVKRPDLKLTLKVDTNAAPAGEGDAAPQAFQIASEATVPAGATFRLA
                     FPFPPEAMNGEDGITVEEYFESYGGLLLKLRYEVDGTQKSLIQYLPPDELKAQLDEVS
                     AQAGG"
     gene            complement(3007..3315)
                     /locus_tag="AC16Y7_01990"
     CDS             complement(3007..3315)
                     /locus_tag="AC16Y7_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708180.1"
                     /translation="MTPPSLRRSYVAMIRIALILAVLLPVGLSACASREEVQSGRVAQ
                     QVAQDAEDDTACRADNEPDTEAYEACRADRTAQRARQSDIDYQKARDFDRVLGGLDNL
                     "
     gene            3431..4993
                     /locus_tag="AC16Y7_01995"
     CDS             3431..4993
                     /locus_tag="AC16Y7_01995"
                     /EC_number="3.4.16.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S10 family peptidase"
                     /protein_id="HIR5708181.1"
                     /translation="MTELIRPLASALSLSALSLGALLVLLAPSAVAVADAPKTEQSDE
                     ADKTPTLRLPPAAITYHTATLDREQVSYSAVAGAIKPRDDRGKPTAEIFYVAYNREPE
                     QTSRPITFVFNGGPGAASAYLHLGAIGPKVVETSAVGELIGPPPRLIGNDASWIDFTD
                     LVFVDPVGTGYSRATGSKTDSDFWGVKQDTDSLAAFIRLYLTQAGRTDSPVFLTGESY
                     GAFRAATITRKLQKTTGISPSGLVLVSPALEFALIYGEDFDPIPWALALPSYTAVKLE
                     NEGVATRGELRDALKDVERYALTDYLVALASGAEQGGRIASARVAELTGISRAEVEKR
                     FARIPLSFFIKEFDRDGGQLLSRYDGSVSGPDPHPSSPWPRGPDPVLDVTKPLWTTAF
                     VTYAQEQLAFKTEQPYELLNGKVRGKWDFGTSPTRQGYAGVIDDIQRARAANRGLEVL
                     IATGYTDLITPYMTATYLVKQLPPLTGASPITIEDYAGGHMLYLRADTRRALKDDVEA
                     MYRRALPVPSEG"
     gene            5072..5671
                     /locus_tag="AC16Y7_02000"
     CDS             5072..5671
                     /locus_tag="AC16Y7_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708182.1"
                     /translation="MPIRRFALATFVLIALVLPGHAEDKAETPDAPQESPWKEFHFTE
                     RGFVASFPVAEKPKAASTPVAGQNPLLQHTYQANLARDTVYSIVVFEYPEGRAPNPPR
                     PDYFIKVVEAYAKGSDTKVRKKASRTFDGRPGYEAVAEDGRGKRNHLIDLVANGDRIY
                     MIVSAGPKGHSRSGDAERFRDGFRLLGGPPPQDSKPDSK"
     gene            complement(5678..>6613)
                     /locus_tag="AC16Y7_02005"
     CDS             complement(5678..>6613)
                     /locus_tag="AC16Y7_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family transporter"
                     /protein_id="HIR5708183.1"
                     /translation="NVFAQDSGQNGGMNRVALVFCLAAGIAALPAVLMRGFTTPTPSE
                     ARGSFLLALPFAGLLLLGAPALGALYEAAAVRAGDALSMIAEGVLTVAALATLLAAGG
                     ALALSVGNVLSYDIYFKSLNPTASVRRQVFVARICVIVVTGLAGLSAIAFPQESLTGA
                     SAMLSLAASTLLPVLVLGVWWKRATADAALAGMVAGTLVCLYYMVGPHTIPFAFYESS
                     SFFSDASDAQVAAYERLRNDYYLAADDAAKAAVLAAWDESVRPITNWFGVRAVFAGLF
                     AVPAGFLVTIVVSLFTRAPSENVRRLVDNLRTRAA"
BASE COUNT         1258 a         2286 c         1995 g         1074 t
ORIGIN      
        1 ttgccccgca aaatcgcata ctcagcggta tagaaattgg cctcgcaacg acctacacct
       61 cgaagggcta cttcgacaat cagatgcata ccggacgcgt tggcgtaacg cggtatttca
      121 attgggggga ctgatcagtt cttcttgccg tagcacttct tcttgaagaa gccgcggttc
      181 atatagcgtg ggttgcgcat gaagggcggc gcggtggtga cgcaattttg cccgccactc
      241 tgcttttggg tggtcttggt gacctgctga ggcttttgct tctgcttcgc caaggccggt
      301 ccggccgttg cgatcgccaa ggccgcaatc agcaggccgg gcagcgcatt gccaattctc
      361 atcatgctcc cctcgcaacg cctcgtttca accgcgcgca gcatgacata cggggccaaa
      421 ccggcgccag cccgatcccc ggccttgcgt cagaccggcg gcaccataat atggccgcgc
      481 tggcggcatg gactgccggc ctcaaagttc gctgcggcgg ggtggcacga aatagcatgg
      541 tgagagttct cttcttggcc ctcgggcttc tgatcgtcgc gtcggcaggc cttcaggcgc
      601 aaacccaatc gctaagacca acgccaaacg cctgcacctc cggcagccag agcgtgcgcg
      661 tctgcaacaa cgacttccag tcttgcaacg acgtctgcgt cgccagggcg ctggatccca
      721 gcaccgagat cgtcggctgc accacagcct gctgctacaa attcaatgtg tgcttgcgga
      781 tgcgcggatg cggcccgcgc gccatcaact gcgactgagc gcgacgtatc aaccgcccgc
      841 ttgcgccgac acctcatcga gctgcgcctt gagctcgtcc ggcggcagat actggatgag
      901 ggacttctgt gtgccgtcga cttcatagcg gagcttcagc agcagcccgc cataggactc
      961 aaaatactcc tccacggtga tgccgtcctc gccgttcatc gcttcgggcg ggaagggaaa
     1021 tgccagccgg aaggtagcac ctgcaggcac ggttgcctcc gacgcgatct gaaacgcttg
     1081 cggggcggca tcgccttcgc cggccggcgc agcgttcgta tcgaccttca gtgtgagttt
     1141 cagatcaggc cgcttcacat ccggcttcaa cacagcctcc accccagtca gcggctgatc
     1201 ggataggttc atcccgccca cggacaggcc gttgatgcga aacgctgccg gtgtccccga
     1261 aagcgtgaac acggcccgat ccgacaacgg acggtcggtc tcctggaacg tcaaagcgat
     1321 ccctacgccg gactcttcct ccgccgcggc cgtcgccgcc gttccggcgc ccgcacgcaa
     1381 caacagcccg ccgccgacca gcagcccgcc acagaccagc aacacagcgc ccgcgatgat
     1441 catcatgcga ctgagatcga catcccttgt ttgtacccac gcctgggact gagccgccca
     1501 agccttcgct tgcacagccg acgagaccgc cacggaccgc atttggcctg cggaggcgcc
     1561 tgcacgcgca ctcgccgcgt gcgcccagga cgagacccac gatagcgcgt ttttcgcctc
     1621 agccggaggc gcgatgggcg ccggagcggc ctccgcaacg cggggctcgt tgggcgagac
     1681 ccccttgacc cagaaccctt catagaacgg tccgtagact tccgcagcct ccgcggcgga
     1741 tttcggttcc tccaccgagg aatggggcga cgaaccgggg tcgttcgccg cacggctggt
     1801 aatctcggcg ccccccacat gcggtccata gagcgcggca gccttcgatg ttttcgccgc
     1861 ggcccgggcc atcatccctt cggcctgcgc tacaccggcc cgcgcatagc gctccgcctg
     1921 gcgccccagc ttggccatgc cgacataaag cgtgggcgtc accgccgccg tcttggccat
     1981 tgtccactcg aagccgttgg cgagaccttt cttgccggcg gcttggaccg ctgcggtctt
     2041 tttgccggca gccgccagcc cgcgcgaggc cgcgtggccc gtgacgcctg caagcgccga
     2101 agtcttggcg gaggcgaccg aaagcccact gcccagcgaa cggccggcgg tgcgcgcagc
     2161 agtgccgctt ttggacgccg cgaataatga ccccgccacc accaaggcgc ccaatcggtg
     2221 cgccagcaca ccgagcttgg cgaccatcca cgacgcgcca gcctggatcc gacggcctgc
     2281 gaccctcgcg ccgcgcgtaa tcagccggcc gacagaaaag attccggccc ggagcgccgt
     2341 tagggcgaag acgtaccagg ccgacacaac caacatgccg gcccaaagcg cgtgccgcat
     2401 ggcttggaag gcacgctgcc gcacgagctg gcggcgaagg cgcagccgct ccttgcgcgc
     2461 ggacgcaaca cgacgatccc gctgacgctg caaccagcgc tcatgccgct gccggtcgcg
     2521 ctccacggcc gcgctctgcg tccggagcca acgctggtga tcggtgagat cggatttggc
     2581 gtcggagaga aacgccccgg atttcgccgc ggtatccttg gcagcggcaa cactcgcctc
     2641 gaaggccgcg gtctcttgcg actcggcgtc gtctgcgcgc gttgaccagg cccgtttctt
     2701 acgcattgca acggccatgc cgtgaggctc cgccaaagat gggatccgcc gcgagcctcg
     2761 taacggcgaa acggctcaac gtggaactcg ccagggggct acaacgccaa agtttgcgaa
     2821 gctccccgcc cccgattctg gagcttcgct acaaggaggc cactcaagac ctctatcggc
     2881 caccaaatga aggcgaggcg gaacggccga tcgccggttc gcccgcctgc gctagcttat
     2941 ccggcaaacg gaccgcggcc aacggccaaa ccttgagggc aaacactttt ggggtgggtg
     3001 gccccgtcac agattgtcga gacccccgag aacccggtcg aaatcacggg ccttctggta
     3061 gtcgatatcc gactgccgcg ctctctgcgc ggtccggtcc gcgcggcacg cctcataggc
     3121 ctcggtatcc ggctcgttgt cggcccggca agccgtatcg tcctccgcat cctgggcgac
     3181 ctgctgtgcc acgcgtccgg actggacctc ctcacggctg gcacaggcag aaagaccaac
     3241 aggcagcaaa acggccaata tcaaggcgat tctgatcatg gcgacgtagc tgcgcctcaa
     3301 tgatggcggg gtcatgatcg aggatcgatc accgggcctc ccgccttgag accgccctcc
     3361 ttgctgcata aatccttccc aaggggagtg aagacgtttc atccggtgtt ttcgaagagg
     3421 acgcgcgccc gtgaccgaac tgatccgccc cctcgcctcc gcactgagcc ttagcgcact
     3481 gagccttggt gcgcttcttg tccttctcgc cccgtctgcc gtcgccgtcg ccgacgcgcc
     3541 aaagaccgaa caatcggacg aagccgacaa gaccccgacc ttgcgcctgc cgccggcggc
     3601 gatcacctac cataccgcca cgctcgacag agaacaggtc tcctatagcg cggtcgccgg
     3661 cgccatcaag cctcgcgacg atcgcggcaa acccacggcg gaaatcttct acgtcgccta
     3721 caaccgggag ccggaacaga cctcccggcc gattacgttc gtcttcaacg gaggaccggg
     3781 cgcggcctcg gcctatcttc accttggcgc catcggaccg aaagtcgtcg agacgtccgc
     3841 cgtcggcgaa ctcatcggtc cgccgccgcg cctcatcggc aatgacgcaa gctggatcga
     3901 cttcaccgac ctcgtcttcg tcgaccccgt cggcacgggc tatagccgcg ccaccggcag
     3961 caagactgac agcgactttt ggggcgtgaa gcaagacacc gactcgctcg ccgcgttcat
     4021 ccggctttac ctcacacaag ccgggcgcac cgactcgccc gtcttcttga ccggcgaaag
     4081 ctatggtgcc ttccgtgccg ccacgatcac gcgcaagctt cagaagacaa caggcatctc
     4141 gccgagcggt cttgtgctgg tctcgccggc gctcgagttc gcgctgatct acggcgagga
     4201 cttcgacccg attccctggg cgctggcgct cccctcttac acggccgtca agcttgagaa
     4261 cgaaggcgtt gcgacgcgtg gggaactgcg cgatgccctg aaggacgtcg aacgctatgc
     4321 gctcaccgat tatctcgtgg cgctcgcgtc cggcgccgaa caaggtggcc ggatagcatc
     4381 ggcgcgcgtg gccgagctga ccggcatctc ccgcgccgaa gtcgagaagc gctttgcccg
     4441 catccccctc tcgttcttca tcaaggagtt cgaccgcgac ggcggccaac tcttgagccg
     4501 ctatgacggc agcgtgagcg gacccgaccc gcacccgtcc agcccgtggc cgcgcggacc
     4561 cgaccccgtt ctcgacgtga ccaagcccct ttggaccacg gcgttcgtga cctatgcgca
     4621 agagcagctc gccttcaaga cggagcagcc atacgagctc ctcaacggca aggtgcgcgg
     4681 gaagtgggac ttcggcacga gcccgacccg tcaaggctat gccggcgtga ttgacgacat
     4741 tcagcgcgct cgcgccgcca accgcgggct cgaagtgctg atcgccaccg gttatacgga
     4801 cctgatcacg ccgtatatga cggccaccta tctggtcaaa caattgccgc cgctcaccgg
     4861 cgcaagcccg atcacgatcg aggattatgc ggggggacac atgctgtatc tgcgtgccga
     4921 cacgcggcgg gccttgaagg acgatgtgga ggccatgtac cggcgggccc ttccggtgcc
     4981 gagcgaaggc tgacagacgg caagcggatg aaccgtcacc accgatgggc tacacttgcg
     5041 attctaacct taggagatgt gaggaccggc gatgccaatt cgacgcttcg ctctcgccac
     5101 ctttgtcctg atcgccctcg ttttgccggg gcacgccgag gacaaggccg agacgccaga
     5161 tgcaccgcaa gagtcaccct ggaaggagtt ccacttcacc gaacgtggct tcgtggcgtc
     5221 ctttcccgtg gccgagaagc ctaaggccgc aagcacgccg gtcgcggggc aaaacccgct
     5281 gctccaacac acctatcaag ccaatctcgc ccgggacacc gtctacagca tcgtggtgtt
     5341 cgaataccct gaaggccggg cgccgaaccc gcctaggccc gattacttca tcaaggtcgt
     5401 tgaggcttac gccaagggca gcgatacaaa agtccgcaag aaggcgtccc ggactttcga
     5461 tggtcgcccg ggctatgagg cggtggccga agatgggcgc ggcaagcgca accacctgat
     5521 cgatctggtg gccaatggcg accggatcta catgatcgtc tccgccggcc cgaagggaca
     5581 cagccgaagc ggcgatgcag agcgctttcg cgatggcttc cgtttgctgg ggggcccacc
     5641 accacaagac tccaagcccg acagcaagta acggcgtcta agcagcacgt gtgcgcaggt
     5701 tgtcgacgag gcggcgcacg ttttccgacg gcgcgcgcgt gaacaggctc acgacgatcg
     5761 taacgagaaa gccggcgggc actgcgaaca atccggcgaa tacggcgcgc acgccgaacc
     5821 aattggtgat cggacgcacc gattcatccc acgccgcgag caccgcggct ttggctgcat
     5881 cgtcggcggc cagataatag tcgttgcgca acctctcgta agcggcgacc tgcgcatcgc
     5941 tcgcatcgga gaagaagctc gacgactcgt agaaggcgaa cggaatcgtg tgcggcccga
     6001 ccatgtagta gaggcacacc aaggtgcctg cgaccatgcc ggcaagcgcg gcgtcggccg
     6061 tcgcacgctt ccaccacaca ccaagcacca gcacgggaag aagcgtgctg gccgcgagcg
     6121 aaagcatggc acttgcgccc gtcaagctct cctgcgggaa ggcgatggcc gaaaggccgg
     6181 cgagccccgt caccacgatc acgcagatgc gcgccacgaa aacttggcgc cgcaccgacg
     6241 ccgtcgggtt caggctctta aaatagatgt cataggagag gacgttcccg accgaaagcg
     6301 cgagcgcgcc gcctgctgca aggagcgtcg caagcgcggc caccgtcaag acgccctcgg
     6361 cgatcatgga aagcgcgtcc ccggcgcgca ccgcggccgc ctcgtacagc gcaccgagtg
     6421 cgggcgcacc gagcaggagc agtccggcga agggcagcgc gagcaaaaac gaaccccgcg
     6481 cctcagatgg cgtcggggtc gtaaagcccc gcatcaggac agccggcaaa gccgcaatac
     6541 cggccgcgag gcaaaagacc agcgccacac ggttcatgcc gccgttttgc ccgctatctt
     6601 gcgcaaagac gtt
//
LOCUS       DBNHVU010000052         9752 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_835922, whole genome shotgun sequence.
ACCESSION   DBNHVU010000052 DBNHVU010000000
VERSION     DBNHVU010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 9752)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9752)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9752
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_835922"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..501)
                     /locus_tag="AC16Y7_02010"
     CDS             complement(136..501)
                     /locus_tag="AC16Y7_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007067181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HIR5708184.1"
                     /translation="MPAPVYKVLVEAALEAAKGEGRFLGTGDDLRDGFIHLSAGHQVE
                     DTLAKHFAGQEDLLLVALDPDRLGAGLKWEPSRGGDLFPHLYGAMPLDAILSANPLPL
                     DDDEQHILPEGFPPEGVAG"
     gene            647..1282
                     /locus_tag="AC16Y7_02015"
     CDS             647..1282
                     /locus_tag="AC16Y7_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HIR5708185.1"
                     /translation="MTRLTHARVWSAIDALAKRYGLSPSGLAKQAGLDPTAFNKSKRV
                     TGEGRPRWPTTESIAKVLRATGADFDDFTALIGAERREPALPIPLIGMTQAGGGGFFD
                     DGGFPVGGGWDHIRFPRVDDDNAYALEVAGDSMAPLYRAGDILIVSPNAAPRKGDRVV
                     LRTTDGEVMAKMLVRRTAKTIELASLNPAHPDLVFPVERIAWIARIIWASQ"
     gene            1336..2076
                     /locus_tag="AC16Y7_02020"
     CDS             1336..2076
                     /locus_tag="AC16Y7_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HIR5708186.1"
                     /translation="MSIASRLVTALVGVLLIWFFWPFGESEEVVQTVTPPPTGHERLF
                     TTKLPEETPPKLAPRAASPEAAPPKAAPKTEAETDGEAPEAKTLEDKTPDDKTPDDTA
                     SEDKASKNTKTAAVAVSPKLKPKRFYRVRVLDSGTLEASGRVIKLDGINPIPSNETCT
                     DKSGTKWLCGTRARLALTRFIRGRAVVCKVPEKDKAKALTARCSLASKDLSVWVAAQG
                     WAKPAAPPKPKIAKAADQARKKKIGIWR"
     gene            complement(2073..3713)
                     /locus_tag="AC16Y7_02025"
     CDS             complement(2073..3713)
                     /locus_tag="AC16Y7_02025"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR5708187.1"
                     /translation="MSKSDTAKQTAPAPIEINSELLAHAHVSKAWPFEEARKVLSRLK
                     RIPEPTGPIIFETGYGPSGLPHIGTFGEVARTTMVRHAFRVLTEDKVPTRLICFSDDL
                     DGLRKVPDNIPNKDIIAEHLGKPLTQVPDPFGEYPSFGQNNNARLKAFLDTFGFDYEF
                     LSATECYTSGRFDATLLKVLENYDAIRDVILPTLGPERRATYSPFLPISPATGKVLQV
                     PVIDRDLASGSIVYKDPDTDKLTHTPVMRGHCKLQWKADWAMRWAALGVDYEMAGKDL
                     IDSVRLSGRICRSLGRQQPEGFNYELFLDENGEKISKSRGNGITIEEWLSYASPESLA
                     LYMYQSPRKAKRLYFDVIPRAVDEYVGHLAAYPKEEQAPKLMNPVWHIHGGNPPEAEL
                     PISFALLLNLASASNSEDRDVLWGFIRRYTPDATPEGHPLLDELVGYAIRYFHDFVKP
                     SKAYRAPSEKERAALTDLDARLAALPEKILAEEIQNEVYAVGKEHGFEPLRDWFQALY
                     QVLLGQTQGPRFGSFVELYGIPETRALIAKALSGELAA"
     gene            3898..4310
                     /locus_tag="AC16Y7_02030"
                     /pseudo
     CDS             3898..4310
                     /locus_tag="AC16Y7_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933597.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
     gene            4426..4701
                     /locus_tag="AC16Y7_02035"
     CDS             4426..4701
                     /locus_tag="AC16Y7_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708188.1"
                     /translation="MWGLERKPSRFARDLDLGDVHDQLDALRGFVQELSRDVGQDARS
                     SYGRARDMAAEAARDAEETMKDNLAASLLLALGLGVAVGYFISRGRK"
     gene            complement(4705..5979)
                     /locus_tag="AC16Y7_02040"
     CDS             complement(4705..5979)
                     /locus_tag="AC16Y7_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708189.1"
                     /translation="MRIATTLALAAIGVLSAATPCAAQSGAVLLAQNSSSVSKSTVSP
                     RRLSPPESPAVQPSSEDTTAREEVAPPPPQQRAVVRTQQIRPVSPQQVAPQHIYARPT
                     LSTPPAGTAPAASSPSEFSSAERLLDWVSNYRDRPEYWKVPDAVHAMRDHGLFKDEEQ
                     RWFCAGFIAGVLGTNPNDGPGLIPRMFPMPPKEQEVIIRAIAYSGRPDWQDLLTKNAD
                     KMPLRQPLIEDFINGRRPTLQKLGLELGGAQAVYAHWGYYAATGQHEPVIRILPALQW
                     SKGASEEGFFGKLTSGWGSTAKNLDMVTTGGTAKWTLASYAERDRDLINLYRSEVPNQ
                     PEEIAKPLNDVIKAAELFEAEKIRKEQYGAIEDAERQQLTAEAGMSKGMTAGSIAIAT
                     GCMAATALGQAQIAVPCVIGGALYSGAAKLSR"
     gene            6382..7311
                     /locus_tag="AC16Y7_02045"
     CDS             6382..7311
                     /locus_tag="AC16Y7_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR5708190.1"
                     /translation="MSIDHANLGRLDLNLLVAFDALFTERSVTRAAARVGLGQSAMSH
                     NLARLRTLFGDELFTRGGDGMRPTPRAMVLADPIRLTLAQVQSAVLQREDFDPATAHR
                     VFRIGLADSIEVAVIPALIERLQAIAPGVELRLSSTNHMSILEELDSGNLDLGIGVFD
                     QGQTHHKRRHLYTDSFLCLFSPEQLEFAPPISLQDYLRWPHVLTSLGEARGAVDEALA
                     KHKLRRNIVVTTPGFMAVPFIVRRSRVITTMPSRLARYFSEAFGLATSPVPIELPNFT
                     ISLLWHTSFDHDPGHLWLRQTVAGVAPDSSLAL"
     gene            7451..8212
                     /locus_tag="AC16Y7_02050"
     CDS             7451..8212
                     /locus_tag="AC16Y7_02050"
                     /inference="COORDINATES: protein motif:HMM:NF015674.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009268 - response to pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HIR5708191.1"
                     /translation="MAETAAAKDRSSTALSEVLADSWWAVGLRGLLGIAFGLICLLVP
                     AAAILALILLFSAYMLVDGVLAIVSGIKAARNGERWGLLILEGIVDLAAGIIAFLWPA
                     ITTVAFVILIAVWAIISGVLMITAALALKIDHGRWWLALAGIASVIFGIVLLVAPVVG
                     AVVLTWWLGAYAIVFGIFLLVLAFQLHGKKEDQATPAKSAARKAPATKTAAKKAPAKK
                     AAAKRAPAKKKTRAKKASARRAPAKKKSRARKARA"
     gene            8293..9219
                     /locus_tag="AC16Y7_02055"
     CDS             8293..9219
                     /locus_tag="AC16Y7_02055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018011619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR5708192.1"
                     /translation="MSQPGRRGTLAAMQNVFDQSDGLIRLGVFAGVFLVMALVEFAWP
                     KRTLVASKGRRWLTNLGISVTANVVLRAMAALAVPIAAIAAAFYAQANGIGLLNAVNW
                     PGWIKIVIALVVLDLAIWAQHLVSHKVPILWRLHKVHHADRDIDVTTAVRFHPFEIAL
                     SMLWKIVVVVPLGASPFAVFLFEVILNGCAMFNHANVALPAWLDRTLRLFIVTPDMHR
                     VHHSVLRREHDSNYGFNLSLWDRLFRTYTAQPEGGHQRMKIGLTPYQSEDPSRFGWSL
                     LLPFWPGGGNTATTEVSKEQLQGGAHRDGDAA"
     gene            complement(9231..>9752)
                     /locus_tag="AC16Y7_02060"
     CDS             complement(9231..>9752)
                     /locus_tag="AC16Y7_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR5708193.1"
                     /translation="EADAAIASIRITEEALKAADFSSRYYATPARFAGRKGSDNQDIR
                     PETVEGLKIGVQKGTGHEAYLKKFFRKSAIAAFDSAEDAQKALGDGAIDLIFGDGVSL
                     TFWLNGAESQACCEFRGGPYLDAKYFGEGVGIAVKKGNRKMAEILTYGLEQVHASGRY
                     EELFLRYFPMSFF"
BASE COUNT         1735 a         3102 c         3055 g         1860 t
ORIGIN      
        1 tctggctcag ccgcggatcg tccggacccg agctgcgggg ataaaccccg agttcgagcg
       61 tcttgagcgt gagatcgtga gcgcgctccg gatcgagcgc cagcagcagc gtctggccca
      121 gcccgaacag cccgctcaac ccgccactcc ttccggcgga aagccctcag gcaggatgtg
      181 ctgctcgtca tcgtcgagcg gcagcggatt ggcggaaagg atcgcatcca gcggcatcgc
      241 gccataaaga tgcgggaaca ggtcgccgcc gcgggacggc tcccatttga gcccagcgcc
      301 cagacggtcg gggtcgagcg cgacgagcag caagtcctcc tggccggcaa aatgcttggc
      361 caaggtgtcc tccacctggt ggcccgcgga cagatggatg aacccgtcac gcaaatcatc
      421 ccctgtccca aggaatcgcc cctcgccctt cgctgcctcc agggcagcct cgacaagcac
      481 tttgtagacg ggcgcgggca tggcgggaga ctagcgggag cggcatgccc caacaaggaa
      541 ggccgtccac ggcggccgaa agggagtgga caacgggagc gtttgagcga taattttaag
      601 aataggtatt tttccctatg agtcccgctt ttccgaggag cctgccgtga caaggctcac
      661 ccatgcccgg gtttggtcgg ccatcgacgc gctggcgaag cgctatggcc tgtcgccgtc
      721 cggactcgcc aagcaggcgg ggctagatcc caccgccttc aacaaatcca agcgggtcac
      781 gggcgagggt cggccccgct ggcccaccac cgagagcatc gccaaggtgc tgcgggcgac
      841 gggggccgat tttgacgact ttaccgccct catcggcgcc gagcgccgcg agccggccct
      901 gccgatcccg ctgatcggca tgacgcaagc gggcggcggc gggttcttcg acgacggcgg
      961 ctttcccgtg ggcggcggct gggaccatat ccgcttcccg cgcgtggacg acgacaacgc
     1021 ctatgcgctc gaagtcgccg gcgacagtat ggcgccgctc taccgggccg gggatatcct
     1081 catcgtttcg ccgaacgccg cgccgcgcaa aggcgaccgc gtggtgctgc gcaccacgga
     1141 cggcgaggtg atggccaaga tgctggtccg gcgcacagcc aagacgatcg agctggcttc
     1201 gctcaacccg gcgcaccccg acctcgtgtt tcccgtggaa cggatcgcct ggatcgcgcg
     1261 cattatctgg gccagccaat aaggcgtaag ggcgctctta aagccaccgc gcaaggcggc
     1321 tatgatctga acgtcatgtc gatcgcgtct cgtcttgtca ccgccctcgt cggcgtattg
     1381 ctcatctggt tcttctggcc gttcggcgag agcgaggaag tggtgcagac cgtgacgccg
     1441 ccgccgaccg ggcatgagcg cctcttcacc acaaaactgc cggaggagac cccgccaaag
     1501 ctggcgccca gagccgcctc acccgaggca gccccaccca aggccgcccc gaagacagaa
     1561 gcagagacgg acggcgaagc tcctgaggcc aaaacgcttg aagacaagac acctgacgac
     1621 aagacgcctg acgacacggc atctgaggac aaggcatcaa agaacacgaa gaccgctgcc
     1681 gtggccgtgt cgcctaagct caaaccgaaa cgcttctacc gcgtgcgggt gctcgacagc
     1741 ggcacgctcg aggcaagcgg ccgcgtcatc aagcttgacg gtatcaaccc gatcccaagc
     1801 aacgaaacct gcactgacaa gagcggcacg aaatggctct gcggcacgcg agcgcggctg
     1861 gcactgacgc ggttcatccg cggccgcgcc gtggtctgca aggtgccgga gaaggataag
     1921 gcgaaggcgc tgacggcacg gtgcagcctg gcgagcaagg acctctccgt gtgggtggcc
     1981 gcgcaagggt gggccaagcc ggccgcgcct ccaaaaccga aaatcgccaa agccgcggac
     2041 caggcgcgca agaagaagat cggtatctgg cgctaagccg cgagctcgcc tgacagcgcc
     2101 tttgcgatca gcgcgcgggt ctcggggatg ccgtacagct ccacgaacga accgaagcgc
     2161 gggccttgcg tctggccgag cagcacctgg tagagcgcct ggaaccaatc gcgcaacggc
     2221 tcgaagccgt gctccttgcc gaccgcatag acctcgttct ggatttcctc ggccaaaatc
     2281 ttctccggca acgcggcgag gcgcgcatcg agatcggtta gcgccgccct ctccttttcg
     2341 gagggcgcgc gataggcctt cgacggcttc acgaaatcat ggaagtagcg gatggcgtag
     2401 cccacgagtt cgtcgagcag aggatgacct tccggcgtcg cgtcgggcgt gtagcggcgt
     2461 atgaagcccc acagcacgtc gcgatcctcc gagttggagg ccgaggcgag attgagcaga
     2521 agcgcgaaac taatgggcag ctcggcttcc ggcgggttgc cgccatggat gtgccacacc
     2581 gggttcatca gcttcggcgc ctgctcttct ttcgggtagg cggcgagatg gccgacatat
     2641 tcgtcgacgg cgcgggggat cacatcgaaa tagagccgct ttgcctttcg cgggctctga
     2701 tacatgtaga gcgcgagact ctccggcgag gcgtagctga gccattcctc gatggtgatg
     2761 ccgttgccgc gcgacttgga gatcttctcg ccgttctcgt cgagaaacag ttcgtagttg
     2821 aagccctcgg gctgctggcg tccgagactg cggcagatgc ggcccgacag gcgcaccgag
     2881 tcgatgaggt ccttgcccgc catttcgtaa tcgacgccaa gcgccgccca gcgcatggcc
     2941 cagtccgcct tccattgcag cttgcaatgg ccgcgcatca cgggtgtatg cgtgagcttg
     3001 tccgtgtcgg ggtccttgta gacgatggag ccggaggcca gatcgcgatc gatgaccggc
     3061 acctgcaaca ccttgccggt cgccggcgag atcggcagga aggggctgta ggtggcgcgg
     3121 cgctccggcc ctaaggtcgg caggatgaca tcgcgaatag cgtcgtagtt ctccagcacc
     3181 tttagcaagg tcgcatcgaa ccgtcccgac gtgtagcact ccgttgctga cagaaactcg
     3241 tagtcgaaac cgaacgtatc gaggaacgct ttcagacgcg cattgttgtt ctggccgaag
     3301 ctcggatact cgccgaaggg atcgggcacc tgtgtcagcg gcttgccgag atgctcggca
     3361 atgatatcct tgttggggat gttgtccggc actttgcgca agccgtccaa gtcgtcggaa
     3421 aaacagatca gccgggtcgg gaccttgtcc tcggtgagca cgcggaaggc gtgacgtacc
     3481 atggtcgtcc gcgccacctc accgaacgtg ccgatatgcg ggagccctga aggcccgtag
     3541 ccggtctcga agatgatcgg cccggtcggc tccgggatcc gtttcagccg gctgagaact
     3601 ttccgcgcct cttcgaaggg ccaggccttg ctcacatggg cgtgggcgag gagctcggag
     3661 ttgatctcaa tgggtgcagg ggccgtctgt ttggccgtat cggacttgga catcggagaa
     3721 gtcagtatcc aacgctgtgg cgggagggta cgcccgacaa atttcacagt ttccggcccg
     3781 atgccagtct ttccggccac tttcctcttg ggctggcgct tgccggaccg taagcaattg
     3841 cctaccgtcg cgccggccta gcccaccaga gggaatctaa agagaggtga tacgccgatg
     3901 acccagcctt tggaccatca cgacgccctg atctacacga tggtcacgat ttcggccgtg
     3961 gatcgcgcca tgaccgacgc cgagctggcc cgtatcggcg agatcgtatc gaatctgccg
     4021 gttttcgacc tgtacgacgc cgacaagctg gtcccggcct cgcgggcttg cggtgagatc
     4081 ctgagcggcg acagcgggct tcagctggtt ctggagttga ttcgcgacac cctgccgacg
     4141 aagctccgcg agacggccta cgccgtcgcg ctcgaggtcg cggcggccga ccttttgtgc
     4201 ggcccgagga aacacgcttc ctcgaaatgc tggccgatac gctcgatctc gacaagctga
     4261 ccacggcggc catcgagcgc ggtatccgct cgcgcaatac ggtcatctaa gccctcttcc
     4321 gcactttacc aatttccaac gctgacgcgc gcgaccggcg cgcgcagctt cacttgttgg
     4381 tatagtcgcg cttccgtcgc gtgttccgat tgaaggaggt ctgttatgtg gggtttggag
     4441 cgaaagccgt cccggttcgc acgggatctc gacctaggcg atgttcacga ccagctcgat
     4501 gcgctgcgcg gcttcgtgca ggaattgtcg cgggacgtcg gacaggacgc caggagctcg
     4561 tacggacgcg cccgcgacat ggcagccgag gcggcgcgcg atgccgaaga gaccatgaag
     4621 gacaaccttg cggcctcgct tctgttggcg ctcggacttg gcgtggcggt cggctacttc
     4681 atcagccgtg ggcgaaaata gcgatcagcg ggacagcttg gccgcgcccg agtagagcgc
     4741 gccgccgatt acgcacggta cggcaatctg ggcttggccc aaggccgtag ccgccatgca
     4801 gcccgtggcg atggcgatcg aacccgcggt catgcctttc gacatgccgg cctcggcggt
     4861 gagctgctgc ctctcggcat cctcgatcgc accgtattgc tctttgcgta tcttctcggc
     4921 ctcgaacagt tcggccgcct tgatcacatc gttcagaggc ttggcgattt cctccggctg
     4981 gttcggaacc tccgagcggt aaaggttgat caggtcgcgg tcgcgttccg cataggacgc
     5041 cagcgtccat ttggcggtgc cgcccgtcgt gaccatgtcg agatttttcg ccgtcgaacc
     5101 ccacccggac gtgagcttgc cgaagaagcc ttcctcgctg gcgcccttgg accattgcaa
     5161 cgccggcaga atgcgtatga ccggctcgtg ctgcccggtg gcggcgtagt agccccaatg
     5221 ggcatagacg gcttgcgccc cgccaagctc caagccgagc ttctgcaagg tcgggcggcg
     5281 cccattgata aagtcctcaa tgagcggctg gcgaagcggc atcttgtcgg cattcttcgt
     5341 cagcaggtcc tgccaatcag ggcgccccga ataggcgatc gcccggatga tgacctcctg
     5401 ctcctttggc ggcatgggaa acatccgggg gatgagcccc gggccatcgt tcggattggt
     5461 accgagcacg cccgcgatga agccggcgca gaaccagcgc tgctcttcgt ctttgaacag
     5521 cccatggtcg cgcatggcgt ggacggcgtc gggcactttc caatattccg ggcgatcccg
     5581 gtagttcgac acccaatcca gcagccgctc ggcggacgag aactccgatg gcgacgacgc
     5641 ggccggtgcc gtgcctgccg gaggcgtgct caaggtcggc cgcgcgtaga tgtgctgagg
     5701 tgccacctgc tgcggtgaga cgggcctgat ctgttgggtc cgcacgacgg cacgctgctg
     5761 gggcggcggc ggggccacct cctcgcgtgc ggtcgtgtcc tcgctggaag gctgcacggc
     5821 tggcgattcc ggtgggctca agcgccgggg cgagaccgtg ctcttggaga cggagctgct
     5881 gttctgcgca aggagcaccg caccggactg agcggcgcaa ggcgttgccg cactgagaac
     5941 gccgatggcg gcgagcgcta aagtcgtggc gatacgcatg tcatcagctt cctcccccag
     6001 accctgaagt gctgacgaat tacacgaata tagttaatgc ccgcgggccc ccggccaacg
     6061 ggcaacatca tgattttcaa tcgtttcgtg gcgatatgaa ggccggccgg ttaaccataa
     6121 caaaaggtta ccgccattta ggctgtttcc aggcctggtt gaccgaccgc cctcccgaac
     6181 tttgcgtcac agaagggcgg ccggggcctc gcacttacgc cgctagggag cgatccttta
     6241 cgcgcgaggg agaccctcgt gtccgttgcg catctcctgg gtcacgttgg cagctcttgt
     6301 ttgtctcaaa acgcgggttc gtttcctgtt gcaagcaatg tgggcgcttc ggtagcatta
     6361 ttccaatcga taggatcgat gatgagtatt gatcacgcta atttaggacg gctcgacctc
     6421 aatctcctgg tcgccttcga tgctcttttt accgagcgca gcgtgacacg cgccgccgcc
     6481 cgcgtcggtc tcggccaatc ggccatgagc cacaacctcg cccgcctgag aacgctcttc
     6541 ggggatgaac tgttcacccg tggcggcgac ggaatgcgcc ccacgccccg ggccatggtc
     6601 ctggcggacc cgatccggct gactttggcg caagttcaat cggccgtgct tcagcgggag
     6661 gacttcgatc cggccacggc ccaccgtgtg tttcggattg ggcttgcaga ctccatcgag
     6721 gtcgctgtca tcccggccct catcgaacgc ctgcaagcca tcgcgcccgg ggttgagctc
     6781 cgcctgagct cgaccaatca catgagcatt ctggaggaac tcgacagcgg caacctggat
     6841 ctcggtattg gcgtgttcga ccagggtcaa acgcatcaca agcgccgcca tctttatacg
     6901 gacagctttc tctgcctgtt cagccccgag cagctcgagt ttgcgccacc tatttccttg
     6961 caagactacc ttcggtggcc gcacgtgctc accagccttg gcgaagcgcg cggcgcggtg
     7021 gatgaagccc tggcaaagca caagcttcgc cgcaatatcg ttgtgactac accaggcttc
     7081 atggccgtac cgttcatcgt tcgccgctca cgcgtcatca cgacgatgcc gtcacgctta
     7141 gcgcggtact tctcagaggc cttcgggctg gccacgagcc ccgtcccgat cgaactgccg
     7201 aacttcacca tctctctgct ctggcataca agtttcgatc acgatccggg acatttgtgg
     7261 ctgcgccaga ccgtggcagg cgttgccccc gacagcagtt tggcgctttg atgcgccctc
     7321 cggatgtgga caaatgccgt tttttccggg cggcggttgc atattagctt catcatcgac
     7381 gcggaattga agcacgtgga gctcgccacc gtggcgaggc tgccggtgca accccgaagg
     7441 agggactttc atggctgaga cagcagctgc aaaggatcga tcgtcaactg cgctgagcga
     7501 agtcttggcc gatagctggt gggccgtagg cttgcggggc cttttgggca tcgctttcgg
     7561 cctgatctgc ctccttgtcc ccgcggccgc gatcctcgcc ctgatcctcc tgttctccgc
     7621 ctacatgctg gtggacggcg tgcttgcgat tgtttcgggc atcaaggccg cccgtaacgg
     7681 cgagcggtgg ggtctgttga tcctcgaagg cattgtcgat cttgccgccg gcatcattgc
     7741 ctttctgtgg ccggcgatca ccacagtcgc ctttgtcatc cttatcgccg tgtgggccat
     7801 catctccggc gtcctaatga tcaccgccgc cctcgccttg aagatcgacc atgggcggtg
     7861 gtggcttgcg cttgccggca tcgcgtctgt gattttcggc atcgtgctgc tcgtggcgcc
     7921 cgtcgtcggt gccgtggtgc tgacatggtg gcttggcgcc tacgcgatcg tgttcggcat
     7981 cttcctcctc gtccttgcct tccagcttca tggtaagaag gaggaccaag ccacgccggc
     8041 gaaaagcgcg gcgcgcaaag cacccgctac aaagacagcc gccaagaagg ccccggcgaa
     8101 aaaggccgcg gctaagaggg ctccagccaa gaagaagacc cgcgccaaga aggcctcggc
     8161 taggagagct ccagccaaga agaagagccg cgccaggaag gctcgcgcct agtagctacg
     8221 aaggggcggc tcgcttgcgc cgccctttcc tctcgtgcct ctgaactcgc gccgttttga
     8281 tcgcgcccat ggttgtcgca gccagggcgg cgcggcacac tggccgccat gcagaacgtc
     8341 ttcgaccaga gcgacggcct gatccgtctc ggcgtctttg ccggcgtttt cctcgtgatg
     8401 gcgctcgtcg agttcgcctg gccgaagcgg acacttgtcg cctcgaaggg gcgccgctgg
     8461 ctcactaatc tcggtatcag cgtgaccgcg aatgtggtgc tgcgggcgat ggcggcgtta
     8521 gccgtgccca tcgcggccat cgccgccgcg ttctacgcac aggcaaacgg gataggactt
     8581 ctcaacgcgg tcaattggcc gggttggatc aagatcgtca ttgcgcttgt ggtgctcgat
     8641 ctcgccattt gggcgcagca tctcgtctcc cacaaggtcc cgatcctctg gcgcttgcac
     8701 aaggtgcatc acgccgatcg cgatatcgac gtcacgacgg cggtgcgttt tcatccgttt
     8761 gagatcgcct tgtcgatgct ctggaagatc gtcgtggtgg tgccgctcgg cgcctcgccg
     8821 tttgcggtgt tcctgttcga ggtcatcctc aatggctgcg ccatgttcaa tcatgccaat
     8881 gtcgcgcttc ccgcttggct cgaccgcact ttgcgtctgt tcatcgtgac ccccgacatg
     8941 catcgcgtgc atcactctgt gctgcgccgg gagcatgact caaactacgg cttcaatctc
     9001 tcgctttggg atcggctgtt ccgcacctac acggcgcaac ccgagggcgg gcatcagcgc
     9061 atgaagatcg gactgacgcc ctaccaaagc gaggacccgt cccgtttcgg ttggagtctg
     9121 ttgctgccgt tttggcccgg gggtggaaac acggccacga cagaggtgtc taaggaacaa
     9181 ctgcaaggcg gcgcacatcg cgacggcgac gccgcctaga aaaagctcat ctagaagaag
     9241 ctcatgggga agtagcgcag gaacagttcc tcgtagcggc cgctcgcgtg cacctgctcc
     9301 agcccatagg tcaggatctc cgccatcttg cggttgccct tcttcacggc aatgccgaca
     9361 ccttctccga aatacttggc atcaaggtac ggcccaccgc gaaactcgca gcacgcttgg
     9421 ctttccgcgc cgtttagcca gaacgtcaaa ctcacgccgt cgccaaagat gagatcgatc
     9481 gcgccgtcgc caagcgcctt ctgtgcgtcc tcggcgctgt cgaaggcggc tatcgcgctc
     9541 ttgcggaaga acttcttcag atacgcctca tggcccgtcc ctttttggac gccaattttc
     9601 aggccctcca cggtttcagg ccggatatct tggttgtcac tgccctttcg gccagcgaag
     9661 cgcgccgggg tggcgtagta tcgggaggaa aagtccgccg ccttaagcgc ctcttcggtg
     9721 atgcggatcg aggcgatcgc cgcatcggcc tc
//
LOCUS       DBNHVU010000053         6747 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1578770, whole genome shotgun sequence.
ACCESSION   DBNHVU010000053 DBNHVU010000000
VERSION     DBNHVU010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6747)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6747)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6747
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1578770"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..2111)
                     /locus_tag="AC16Y7_02065"
     CDS             complement(63..2111)
                     /locus_tag="AC16Y7_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR5708194.1"
                     /translation="MENGPGNQLKAETSPYLLQHKDNPVHWRAWGREAFAEAKAANKP
                     ILLSIGYAACHWCHVMAHESFEDEETAKVMNDLFVNIKVDREERPDVDTIYMSALHRI
                     GEQGGWPLTMFLTPDGEPFWGGTYFPKDARYGRPAFVSVLTTVAQVFRDEPDKVRSNA
                     DAITEALRPRAQDGGASMAINDETLANAATRFLQAVDPVTGGMRGAPKFPQTQFFAFL
                     WRAALRYGLSNPREAVHVTLTNIAQGGIYDHLGGGFARYSVDEQWLVPHFEKMLYDNA
                     LLVELLTEAWREDKTRLYAERVNETVDWLLREMVTKDGAFASSLDADSEGEEGKFYVW
                     SEAEIVDVLGADDAQLFAEVYGVTEGGNFEGQNILNRLDALKLRDDKTEAKLAELRSK
                     LLARREARVRPGFDDKVLADWNGLMITALANAGAAFDKPEWLAAAEAAFDFVFTRMTT
                     DGRLTHAYRDGRAKAPATASDYANMIAAALALTNADNNPEYVEQACAWTHILDEHYWS
                     DELGGYCLAADDTDDLIVRPFSGLDDATPNANAMMISNLIALSLWTGEARYVERAHEM
                     LRRFGVALTGNPVAHSGMLAAAIDTMAPALVVLMVPEGGHVAGMRAALRDVSLPNVVV
                     DEVWGDETLPSSSPAFGKAPVDGKTTAYVCLGPQCSAPVTEPEKLVETIKSARQVAVH
                     "
     gene            2129..3469
                     /gene="mnmE"
                     /locus_tag="AC16Y7_02070"
     CDS             2129..3469
                     /gene="mnmE"
                     /locus_tag="AC16Y7_02070"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR5708195.1"
                     /translation="MSRTIADQAVPESGGTIVAPASGAGPSAVAVVRVSGPRTRAVLE
                     ALVGTVPEPRQASLRKLADIDRGLVLWFPAPRSFTGEDMAELQVHGSRAVVQALVEAV
                     LEVEGTRLAEPGEFARRAFENGKLDLTEVEGLADLVNAETEAQRRQALAQSEGALRRL
                     YEGWRAELVQAQSLMEAGLDFADEADVAANVSAKAAAIVVPLLQSIESHLADRSGEVL
                     RDGLRVVIAGPTNAGKSSLLNALAKRDVAIVSEEAGTTRDVIEVHLDVGGLPVILADT
                     AGIRSGISPVETEGIRRAFARAEDADLVLWVVDGTSPVFEPPEALAQKPILRALNKAD
                     ITPELAPKDAAKCPLRLSAKTGEGLEGLIGGLEAAARDRIGASSGAPITRARHRAELV
                     STADALKRFVNHDLGPELAAEELRLAASHLGRLTGTIGVEEVLGAIFAEFCIGK"
     gene            3578..5461
                     /gene="mnmG"
                     /locus_tag="AC16Y7_02075"
     CDS             3578..5461
                     /gene="mnmG"
                     /locus_tag="AC16Y7_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIR5708196.1"
                     /translation="MSQVSRGKNFDVIVIGGGHAGCEAASASARMGAATALLTHRFDT
                     IGEMSCNPAIGGLGKGHLVREIDALDGLMGKVADAAGIQFRVLNKSKGPAVQGPRAQA
                     DRKLYRAAMQGAISETPNLEVIEAAVDDIVVEAGEVTGVVTSDGRHFGAGAVVLTTGT
                     FLRGLIHIGETKIPAGRMGEAPALGLSERLYGLGLRMGRLKTGTPPRLDGRTIQWNRL
                     QMQPGDTRPVPFSFLTKEITTPQVPCHITHTSEETHRVIAGNLDRAPTYSGDIDSVGP
                     RYCPSIEDKVVRFKGRGSHQIFLEPEGLDDHTVYPNGISTSLPEDVQHAFLKTIPGLE
                     QAEVIRPGYAIEYDYVDPRELHISLQTKAVARLFLAGQINGTTGYEEAAGQGLVAGVN
                     AALTASGAADGFTVTRADGYLGVMIDDLVTRGVTEPYRMFTSRAEYRLMLRADNADQR
                     LTPLGLDIGCVGETRRVAFAAKTAKLEAGAALLRGLTLTPDEAAKAGLTINRDGRRRS
                     AFDLLSFPEIDLPRLAGIWPEIGELDAVTARQLAVDARYAVYIARQKMDVAAFRKDEA
                     IAIPDGFAYDGLQGLSNELRQKLESHKPASLGQAARLDGMTPAALMLLLAHLKKGAAA
                     KSA"
     gene            5458..6123
                     /gene="rsmG"
                     /locus_tag="AC16Y7_02080"
     CDS             5458..6123
                     /gene="rsmG"
                     /locus_tag="AC16Y7_02080"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR5708197.1"
                     /translation="MSARRKGPILIDGPESFAEAFNVPHETIHRLTRYADLLAHWQKS
                     INLVAPLTLPELWSRHFADSAQLLGLAPEARLWLDLGSGAGFPGLVVAILQKDLPDFR
                     MSLVESNTKKCAFLAEVVRATEAPVDIHAMRIEQLAEKAQSLIPDVVCARALAPLPRL
                     LELAEPFFGEGTRGLFLKGRATEAEVEAAKAGWDFKSRRHRSLTADDAFIVEVTNVRR
                     RVS"
     gene            6120..>6747
                     /locus_tag="AC16Y7_02085"
     CDS             6120..>6747
                     /locus_tag="AC16Y7_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011942685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR5708198.1"
                     /translation="MSRAKTRIIALANQKGGVGKTTTAINLGTALAAVGERVLIVDLD
                     PQGNASTGLGIEAREKSTFDVLTGDATIMEAKVATHVPRLSIVPATIDLMSFERESAN
                     ANGKHYRFRDCLAALAAAEDPEHRTTYVLVDCPPSLNLLTLNALAAADAVLVPLQCEF
                     FALEGLAQLLSTVEEIRVRLNPKLRIHGVVLTMYDQRTSLSDQVVEDVR"
BASE COUNT         1188 a         2218 c         2138 g         1203 t
ORIGIN      
        1 gggcaccatg gacgcgatcg agtgccgcca tcgactctgg aacctgaggt tatttgagtc
       61 aatcaatgca ccgccacctg ccgcgcggac ttgatcgtct cgacgagctt ctcgggctcg
      121 gtgacgggcg ccgagcattg cgggcctaag cacacatagg cggttgtctt gccgtcgact
      181 ggggctttgc cgaacgcggg cgatgaggac ggcagcgtct cgtcgcccca gacctcgtcc
      241 accacgacat tgggcagcga cacgtcgcga agcgcggcgc gcatccctgc gacgtgtccg
      301 ccctcaggca ccatgaggac gacgagtgcc ggcgccatcg tgtcgatcgc cgcggcgagc
      361 atgcccgaat gcgccacggg gttgcctgtg agcgcaacac cgaaacgccg cagcatctca
      421 tgggcgcgtt ccacgtagcg cgcttcgccg gtccacaacg atagtgcgat gaggttcgag
      481 atcatcatgg cgttggcgtt gggtgttgcg tcatcgaggc cggagaacgg ccgcacgatg
      541 agatcgtccg tgtcgtcggc ggcaagacag tatccgccaa gctcatccga ccagtagtgc
      601 tcgtcgagaa tgtgcgtcca ggcgcaggcc tgttccacat attccggatt gttgtccgcg
      661 ttggtgagcg ccagtgcggc cgcgatcata ttggcgtagt cgctcgcggt ggccggcgcc
      721 ttggcacgcc cgtcgcgata agcgtgggtg aggcgcccgt cagtggtcat tcgtgtaaag
      781 acgaaatcga acgcggcctc cgcagcggcg agccattcgg gtttatcgaa ggcggcgccg
      841 gcattggcga gcgctgtgat catgagtccg ttccagtcgg ccagcacttt gtcgtcgaag
      901 ccgggacgca cgcgggcttc acgacgcgct aagagtttgc tgcgcagctc ggcaagcttt
      961 gcttccgtct tatcgtcgcg caatttgagc gcatcgagcc gattgagaat gttttggcct
     1021 tcgaaattgc cgccctcggt aacgccgtag acttcggcga agagttgcgc atcgtccgcg
     1081 cccaaaacat cgacaatttc ggcttcgctc cacacataga acttgccttc ctcgccttcg
     1141 gaatccgcat cgagcgatga ggcgaacgcg ccgtccttcg tcaccatctc gcgcaatagc
     1201 caatcgaccg tctcgttcac gcgctcagcg taaagccgtg tcttgtcctc gcgccaggcc
     1261 tcggtgagaa gctcgacaag aagcgcgttg tcgtaaagca tcttctcgaa atgcggcacg
     1321 agccattgtt cgtcgacgct gtagcgcgcg aagccgccgc cgagatgatc gtagatgccg
     1381 ccttgcgcga tgttcgtcag cgtgacgtgc acggcctcgc gcgggttcga taaaccgtag
     1441 cgcaaggcag cgcgccacag gaaggcgaag aactgcgttt gcggaaactt cggtgcgccg
     1501 cgcatgccgc cggtcaccgg atcgacggct tgcagaaatc gcgtggcagc attggcgagt
     1561 gtttcgtcgt tgatcgccat ggacgcgccg ccgtcctggg cgcgggggcg taaggcttcg
     1621 gtgatcgcgt cggcattgct gcggaccttg tccggttcgt cgcggaagac ctgcgccaca
     1681 gtagtcagta cactgacgaa agcgggtctc ccatagcggg cgtccttcgg aaaataggtg
     1741 ccgccccaga agggctcccc gtcgggcgtg aggaacatgg tcagcggcca gccgccctgt
     1801 tcgccgatcc ggtgcagcgc gctcatgtag atcgtgtcga cgtccgggcg ctcctcgcgg
     1861 tcgaccttga tgttgacgaa caggtcgttc atcaccttgg cggtctcttc gtcctcgaag
     1921 ctctcatggg ccatcacatg gcaccaatgg caggcggcgt agccgatcga caggaggatg
     1981 ggcttgttcg ctgccttggc ttcggcgaac gcctcccgtc cccaggcgcg ccaatggaca
     2041 ggattgtcct tgtgctgcag gaggtagggg ctggtctccg ccttgagctg attaccgggg
     2101 ccgttttcca tggcctcaag gatactccat gagccgcacg atcgcagatc aagctgtgcc
     2161 cgaaagcggc ggcaccatcg tcgcgccggc gtcaggtgcc gggccttcag ccgttgcggt
     2221 ggtccgcgtg tcgggtccgc gcacgcgcgc cgtgctcgaa gcgctcgtcg ggactgtgcc
     2281 cgagccgcgc caggcgagct tgcggaagct ggccgacatc gaccgcggtc tcgttttgtg
     2341 gtttccggcc ccgcgcagct ttaccggcga agacatggcc gagcttcagg ttcatggcag
     2401 ccgcgccgtg gtgcaggcgc ttgtggaggc ggtgctcgaa gttgaaggca cgcgcctcgc
     2461 cgagcccggc gagttcgcaa ggcgagcctt cgagaacggc aagctcgatc tcaccgaggt
     2521 cgaggggctg gccgatctcg tcaatgcgga gaccgaagcc cagaggcgtc aggcgctggc
     2581 ccaaagcgaa ggcgcgctcc gccgtctcta tgaaggctgg cgcgccgagc tggttcaagc
     2641 gcaaagcctc atggaagccg ggctcgactt cgccgacgag gccgatgtcg cggccaatgt
     2701 cagcgccaag gccgccgcga tcgtggtgcc gctgcttcag tcgattgaga gccatcttgc
     2761 tgaccgcagc ggcgaggtgt tgcgcgatgg cctccgggtc gtcatcgcag ggccaaccaa
     2821 tgccggcaag tcgtcgctcc tcaatgcgct ggccaagcgc gatgtcgcca tcgtctccga
     2881 agaggccggc accacccgcg acgtgatcga agtgcatctg gatgttggcg ggctcccggt
     2941 catccttgcg gatactgcgg ggataagatc ggggataagt ccggtcgaga ccgaaggcat
     3001 aaggcgggcc ttcgctcggg cggaggacgc cgatctcgtc ctctgggtcg tcgacgggac
     3061 gagcccggtt ttcgagcccc cggaggcgct tgcccaaaag ccaattctgc gtgccctgaa
     3121 taaggccgat atcaccccgg aattggcccc taaagacgcc gcaaaatgcc ctttgcgcct
     3181 atcggccaag accggcgaag ggctcgaggg cttgattggc ggcctggagg cggccgcacg
     3241 ggacagaatc ggggcttcta gtggggcccc catcaccagg gcccggcacc gggccgaact
     3301 tgtgtcaacg gcggacgccc tgaaacgctt tgttaaccat gatctcggcc ccgagctcgc
     3361 cgccgaggag ctccgccttg ccgccagcca tctcggccgc ctcaccggca cgatcggcgt
     3421 ggaggaggtg ctgggcgcca tcttcgccga gttctgcatc ggcaagtagc tcggcaagga
     3481 gcgcggcacg tagccgccac cgcgttgatt tgattcacgt gaaacactcc ccggccggcg
     3541 ctttgacggg agccctccga cccactactt accgcccatg agccaggttt ctaggggcaa
     3601 gaactttgac gtcatcgtca tcggtggtgg ccatgcgggc tgcgaggccg cttccgcttc
     3661 cgcgcgcatg ggcgcggcaa ctgccctcct cacccaccgt ttcgatacga tcggcgagat
     3721 gtcctgcaac cccgccattg gcggtctcgg caagggccat ctggtgcgcg agatcgatgc
     3781 cctcgacggt ctcatgggca aagtggcgga tgcagccgga atccagttcc gcgtcctgaa
     3841 caagtccaag ggccctgccg ttcaaggccc ccgcgcccag gccgaccgca agctctaccg
     3901 cgcggccatg caaggcgcga tctccgagac ccccaatctc gaagtcatcg aggccgcggt
     3961 cgacgacatc gtagtcgagg ccggcgaggt cacaggtgtg gtgacgtcgg acggccgcca
     4021 cttcggcgcc ggcgccgtgg tgctgaccac cggcacgttc ctgcgcgggc tcatccatat
     4081 tggcgaaacg aagatccccg ccggccgcat gggcgaggcc ccggccctcg gtctctccga
     4141 gcggctttac gggctgggac tgcgcatggg gcgcctgaag accggtacgc cgcctcgcct
     4201 cgacggccgc accattcaat ggaaccgcct gcaaatgcag ccgggcgata cgcggcccgt
     4261 gccattctcc ttcctcacca aagagatcac cacgccccag gtgccctgcc acatcacgca
     4321 cacgagcgaa gagacgcatc gcgtcatcgc tggcaatctc gaccgcgcgc ccacctattc
     4381 gggcgacatc gatagcgtcg gcccgcgcta ttgcccctcc atcgaagaca aggtcgtccg
     4441 cttcaagggg cgtggcagcc atcagatctt cctcgagccc gaaggcctcg acgaccacac
     4501 agtctatccc aacggcattt cgacctcgct ccccgaggac gtgcagcacg ccttcctcaa
     4561 gaccattcct gggctggagc aagccgaagt catccggccc ggctacgcca ttgagtacga
     4621 ctacgtggac ccacgcgagc ttcacatcag ccttcagacc aaggccgtgg cgcggctgtt
     4681 tctcgccggc cagatcaacg gcaccacggg ctacgaggag gcggcaggcc agggtctggt
     4741 cgcgggcgtc aatgccgcgc tgaccgcctc cggcgctgcg gacggtttca ctgtcacccg
     4801 cgcggatggc tatctcggcg tgatgatcga tgacctcgtc acgcgtggtg tcaccgagcc
     4861 gtaccgcatg ttcacgagcc gtgcggagta ccggctgatg ttgcgtgccg acaatgccga
     4921 ccagcgcctc acgccgcttg ggctcgacat tggctgtgtc ggtgaaacgc gccgtgtcgc
     4981 gttcgccgca aagacggcga agctcgaggc cggcgccgca ctgctgcgcg gtctcaccct
     5041 gacgccggac gaagccgcga aggcagggct caccatcaat cgcgatggac gccggcgcag
     5101 cgccttcgac ctactcagct tccccgagat cgatctgccc cgcctggccg gtatctggcc
     5161 ggagattggt gagctcgacg ccgtcaccgc gcgccagctc gcagtcgatg cgcgctacgc
     5221 cgtctacatc gcgcgccaaa aaatggacgt cgccgcgttc cgtaaggacg aggcaatcgc
     5281 cattccggac ggcttcgctt acgacggtct gcaggggctc tccaacgagc tccgccagaa
     5341 gctcgaaagc cacaagcccg caagcttggg ccaggcagcg cgtctcgatg gcatgacgcc
     5401 ggctgccttg atgctgctcc tcgcgcatct caaaaaaggc gcagcggcga aaagcgcatg
     5461 agtgcgcggc gtaaagggcc catcctcatc gacggtccgg agtcgttcgc cgaagccttt
     5521 aatgttccac atgaaacaat tcacaggctg acccgctacg cggacctgct cgcccactgg
     5581 cagaaatcca tcaatctcgt cgcgcccttg acgctcccag agctgtggtc gcggcacttc
     5641 gccgactccg cgcaactgct tggccttgcg cctgaggcgc gactctggct cgatcttgga
     5701 tcgggcgccg gctttccggg cctggtggtt gctattctgc agaaagacct gccggatttc
     5761 cgcatgagcc tggtcgagtc gaataccaag aaatgcgcgt ttctcgccga ggtcgtgcgc
     5821 gccacggagg cccctgtgga catccacgct atgcgtattg aacagcttgc cgaaaaagct
     5881 caaagcctaa taccagacgt ggtctgcgcc cgcgcgctcg cgccgctgcc gcgtctccta
     5941 gagctcgcag agccgttctt cggggaagga accagggggc tgttcctgaa aggtcgcgcg
     6001 acagaggccg aggttgaggc cgcgaaagcc gggtgggatt ttaagtcccg ccggcatcgc
     6061 agcctgacgg ctgacgacgc cttcatcgtc gaggtgacga acgtgcgccg gagagtgtca
     6121 tgagccgcgc caagacacgc atcatcgcac tcgccaatca gaagggcggc gtcggcaaga
     6181 ccacgaccgc catcaatctc ggtacggcct tggccgccgt cggagagcga gtgctgatcg
     6241 tcgatctcga cccgcaaggc aacgcctcga ccggacttgg catcgaggcc cgcgagaagt
     6301 ccaccttcga cgttctgaca ggcgatgcga cgatcatgga agccaaggtg gcgacccatg
     6361 ttccgcggct ttccatcgtt cccgcaacga tagatctcat gtcgttcgag cgggaatccg
     6421 caaacgccaa cggcaagcac taccgcttcc gcgactgcct tgccgcgctg gccgccgccg
     6481 aggacccgga acatcggacc acctatgttc tcgtcgactg cccgccgtcg cttaatctgc
     6541 tgacgttaaa tgcgctggcg gccgcggacg ccgtcttggt cccactgcaa tgtgagttct
     6601 ttgcgcttga aggcctcgcg cagctcctca gcaccgtcga agagatccgg gttcggctga
     6661 acccgaagct gcgcatccac ggcgtggttc tcaccatgta cgaccagcgc accagcctgt
     6721 ccgaccaggt ggtggaggac gtgcgcg
//
LOCUS       DBNHVU010000054        11556 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_316849, whole genome shotgun sequence.
ACCESSION   DBNHVU010000054 DBNHVU010000000
VERSION     DBNHVU010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 11556)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11556)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11556
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_316849"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1612)
                     /locus_tag="AC16Y7_02090"
     CDS             complement(<1..1612)
                     /locus_tag="AC16Y7_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708199.1"
                     /translation="MAQKQAKSAPGKETSDDKEEVKGPLEAMMQGKTKGKSDDLERMS
                     TLPRRSTLADRLQAEQRKRVEPKREEPRREEPRREEPTPANLSAPQEEAKPADAPEAA
                     GAPSPKHLSRSRRPAGPPPQRRLATPANDDVPSIGGLIFALQQRPAHTPFLFALVASV
                     VWFVIGGIFAYGVISNAAASGGFLSAPALAATAAILVPIIIFWFLALLVWRAQELRLM
                     ASAMTEVAVRLAEPDRLAEQSVASVGQTIRRQVAAMNDAISRAIGRASELEAMVHSEV
                     AALERSYGENELRVRGLINELATEREALTNNSQRVSEALKGVGAQVARDISNASGAID
                     QKLAERGTQLTELLVSRSNEAADQVHKAQAKVSEQVPGLLERLSKEQGRLTKVIEGAT
                     QNLTSLETVVAQRTTALDETMKERTEALQSSLAERIKGLETSVAQGAIVLDKTLKERA
                     ETFMAEIGKSTQVLDDALKNRTNAFTGLVAEGTVAIEKTMADKTEALTNVVAESAVTL
                     DKTLEDRTAALTNVVAESAVALEKTMTDK"
     gene            2005..2400
                     /locus_tag="AC16Y7_02095"
     CDS             2005..2400
                     /locus_tag="AC16Y7_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013767.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_function: GO:0043424 - protein histidine kinase binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR5708200.1"
                     /translation="MASKTAAEVQASTPANEQGPSGNPVDFEHLARYTLGEPELEREV
                     LGLFCSQSVLLLDQLRAAKSDEDWKQAAHSLKGSARAIGAWRMAQAAEQAEALQGDGL
                     SELRSARVDEVEASLREAEAYIATVLESR"
     gene            2577..2897
                     /locus_tag="AC16Y7_02100"
     CDS             2577..2897
                     /locus_tag="AC16Y7_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR5708201.1"
                     /translation="MARITYIQPDGTENVVDADPGMTVMEAAVKNSVRGIAAECGGAC
                     ACATCHVYVEDAWREPTGQPEQMEEDMLDFAFDIRPESRLSCQIKVTEELDGLRVRIP
                     EKQF"
     gene            2904..3932
                     /locus_tag="AC16Y7_02105"
     CDS             2904..3932
                     /locus_tag="AC16Y7_02105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5708202.1"
                     /translation="MSAEVIKTDAVIIGAGPVGLFAVFELGLVDVKAHVIDILDRPGG
                     QCAELYPEKPIYDVPALPVVTGQELTDKLMEQIKPFGAEFHFSERVETLERTDEGFHL
                     VTDADTAFECKVVVIAAGGGSFTPKRPPLPGIEAYEGTSVYYSVRKMDVFKDKDVLIV
                     GGGDSALDWTLNLQPVAKSLTLLHRRSEFRAAPASVQKMLDLVENGDAAFKLGQVTEL
                     HGDHGQLTGVTVKGPDGATFEQSADVMLPFFGLTMKLGPVADWGLNLEENLIPVDTEK
                     FETSETGIFAIGDINTYPGKLKLILSGFHEAALMAQQVHKIVYPDKKLIFQYTTSSSN
                     LQKKLGVK"
     gene            complement(3956..4942)
                     /locus_tag="AC16Y7_02110"
     CDS             complement(3956..4942)
                     /locus_tag="AC16Y7_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708203.1"
                     /translation="MTVAPRGPGRTAPQGGTSSGGHTKTAAVGAMSRTKDSNTNAAAL
                     GRGALRNRLAGRLAQLADKTSSQTKQATKDGLARAAEYGRGSVLPEIERTGAKIKEHA
                     RPERIKQDYKAFLHWLNARVFDPPKEQLFFSSTKDPVALSGLTIRSRHPERGHDYRPT
                     PCQLFQWTLAGIDHDFSKLTFVDHGAGKGRVLLLAAEHPFAAVGGIEFAEELHDDAAM
                     NIAQYPRSHMRCRNVECVLGDATEVGPPEGEAIHYFFNPFSREAFAEVLNNIVVSYRD
                     KPRRLYLVLVDPIATDLLDESGVFARVEIPANERLKIRLFSPYDVAIYRTLA"
     gene            5115..5669
                     /locus_tag="AC16Y7_02115"
     CDS             5115..5669
                     /locus_tag="AC16Y7_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708204.1"
                     /translation="MQAEDFHDSVRPGLIAMLPRLMRFADVLVGARDEGAGLLSRALQ
                     LMLAEQHRYQRGTSLDVWAFAEIYRYWLSELRDHAEPVAQARIDETRFVELFYAKGDD
                     YVDASVVAFLGSLSAPQRLTVLLVYGEGFEYEDASRILDVPPETIAARLIRISAVLAD
                     RLNARAPAQAAATVETLYPEGLRG"
     gene            5672..5983
                     /locus_tag="AC16Y7_02120"
     CDS             5672..5983
                     /locus_tag="AC16Y7_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR5708205.1"
                     /translation="MTELSDELLIAYVDGQLARKQTRAVEGVLDQDDVLAARVDALKH
                     AHARLEAAFEAILAGEEMDISEVPVPQDDGIFVSWRTIKIATAGLGLAVAAALAVAGY
                     G"
     gene            complement(6076..6528)
                     /locus_tag="AC16Y7_02125"
     CDS             complement(6076..6528)
                     /locus_tag="AC16Y7_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708206.1"
                     /translation="MKHPFSTATPIAAAAAAALLVALTACSTVDSIRGPETGASTEAA
                     AYNVRPNDPLARPTQVAWTSARATRCGFVFNPQQLRSNYLAYESRFGHGQARMAKTTH
                     AYDYTLESVFQQIKNDQGYCTKDRLDAIRVDLNRHLSGDYRAIARMAR"
     gene            7252..8088
                     /locus_tag="AC16Y7_02130"
     CDS             7252..8088
                     /locus_tag="AC16Y7_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708207.1"
                     /translation="MPGLVGTRARRLLLAAGAAVLGGLLALGADVSGPGGASLDMRNL
                     VGGETVVSKHAKTKGPAAASAADGFEPTSVSRLLTMALAPPLPSPAQDAEPNLLGLSE
                     IPRELIWNRPPKKDDGKKRRAFAAFSKASANLPWDAVEPVPFSPLGPTGKSAQKKTSP
                     NGAAPVAQAAVVLPAGGEVKQWIKSKVTEIKGSARSRPLYHFELWLEPPAEMKQRLVG
                     VSYAFNSPAIRPQLQASSDKTNGFRISAGGLACADEVKLTLRFNDGGSQTVALDGCNL
                     LS"
     gene            complement(8093..9073)
                     /locus_tag="AC16Y7_02135"
     CDS             complement(8093..9073)
                     /locus_tag="AC16Y7_02135"
                     /inference="COORDINATES: protein motif:HMM:TIGR01620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HIR5708208.1"
                     /translation="MTAPKRRKPAAFRVEDVDVFVPPEPPAAETLPTPMDAQTRMLPN
                     LDRGLRWGSIFLAALGGLISLIASLWLYDWVLSLLARDDWIGWVAVGLLAVVVLALLM
                     IIIREVAGLLRLGRLDKIRHEADSAARQNDKALAVDVTRRLKRFYAGRDDLAWSLQRL
                     AEHEHDIMNAEELLRLDERTLIAPLDPVARGIVATSAKRVSVVTAVSPNALIDMMFVG
                     AQNLRMLRKLATLYGARPGTLSLLKLARMVVTHIVLTGGIALGDDVIQQVIGHGLTAR
                     LSARLGEGVFNGALTTRIGIAAIDVCRPLPYIEQPRPRFRELVAEVAGIK"
     gene            complement(9070..10527)
                     /locus_tag="AC16Y7_02140"
     CDS             complement(9070..10527)
                     /locus_tag="AC16Y7_02140"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019003 - GDP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family GTP-binding protein"
                     /protein_id="HIR5708209.1"
                     /translation="MPLSTYTQAAGDAVRNVGDVASGLATPRLRLGVTGLARSGKTVF
                     ITALVHNLIASGRLPFFDAGAEGRLIRVYLEPQPDEIVPRFEYERHLADLTAEPPTWP
                     ESTRQISQLRLTLEYASTRFWKRQLGRERLHIDIVDYPGEWLLDLPLLQRDYASWARD
                     AVAQAREADREAAAKDWNTALGEIDPAEPADEALAAKLADLFKTYLREARSDQYALST
                     LPPGRFLMPGDLAGSPALTFAPLDAKGADSFPRGSLWSLMERRYEAYKTYVVRPFFRD
                     HFARLDRQVVLIDVLAALNGGPAAVADLERAMTEILECFRTGANNLWSNLFAPRIDRI
                     ALAATKADHLHHESHDRLEAILSLLTDKALARARYRGADVNVLALAAIRATQEGEAKR
                     GTQTLPCIVGYPLKGEKIGRRIFDGNEPFAIFPGDLPEDPKKALSGWQTEHGNMRFVR
                     FRPPNPVQLPSGGVAPFPNIRLDRAIQTLIGDRLA"
     gene            10866..11300
                     /gene="dksA"
                     /locus_tag="AC16Y7_02145"
     CDS             10866..11300
                     /gene="dksA"
                     /locus_tag="AC16Y7_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HIR5708210.1"
                     /translation="MATARKKKKVLPEDYRPSDDEPFMNEQQRAYFRRKLLNWRDEIL
                     RSTKETLQHLQDDSEQYADIADRATSETDRSLELRARDRQRKLIAKIDDALERIEGGT
                     YGYCEETGEPISLKRLDARPIATLSVEAQERHERRERVYRDV"
     gene            complement(11338..>11556)
                     /locus_tag="AC16Y7_02150"
     CDS             complement(11338..>11556)
                     /locus_tag="AC16Y7_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5708211.1"
                     /translation="AFFYAVGTGVGGIGAPWLFGALIDTGSRMSLFAGYLLGAGLMLA
                     ASVIAARYGVAAEGKSLEDVAKPLSAAH"
BASE COUNT         2051 a         3611 c         3782 g         2112 t
ORIGIN      
        1 tcttgtccgt catcgtcttc tcaagcgcca ccgcgctttc ggccacgaca ttggtcaagg
       61 ccgccgtgcg gtcttccaag gtcttgtcga gggtgacggc gctttcggcg acgacgttgg
      121 tgagcgcctc ggtcttgtcc gccatcgtct tctcgatcgc caccgtgccc tcggccacga
      181 gaccggtgaa cgcgttggtg cggttcttca gcgcgtcgtc gagcacctgc gtgctcttgc
      241 cgatctcggc catgaacgtc tcggcgcgct ccttgagcgt cttgtcgagg acgatggcgc
      301 cctgtgcgac cgacgtctca agtcccttga tgcgctccgc aagcgagctc tgcagggctt
      361 ccgtccgctc cttcatggtc tcgtcgagag ccgtggtgcg ctgtgcaaca acggtctcca
      421 gagaggtcag gttctgggtc gcgccttcga tcaccttcgt caggcgcccc tgttccttgc
      481 taaggcgctc cagcaggccc ggcacttgct cggacacctt agcttgcgcc ttgtggactt
      541 gatcggcggc ttcgttggag cgggacacga ggagctcggt gagctgtgtg ccgcgctcgg
      601 ccagcttctg gtcgatcgca ccgctcgcgt tggagatatc gcgcgccacc tgggcgccca
      661 cgcccttcag ggcctcgctt acgcgctggc tgttattggt gagggcctcg cgctcggtgg
      721 cgagttcgtt gatcaggccc ctgacgcgaa gctcgttctc gccgtaggag cgttcgagcg
      781 ccgcgacctc gctatgcacc atggcttcga gctcgctggc ccggccaatg gcgcgcgaaa
      841 tagcgtcgtt catcgccgcg acttggcggc ggatggtctg acccacagag gccacggact
      901 gctcggccag ccggtccggc tcggcgagcc tgacggcgac ctcggtcatg gccgaggcca
      961 tgaggcgcag ctcctgggcc cgccatacga ggagggcgag gaaccagaag atgatgatgg
     1021 gaaccagaat ggcggccgtg gcggcgaggg ccggcgcact caggaagccg ccgctggcgg
     1081 ctgcgttgga gatcacgcca taggcgaaaa tgccgccaat gacgaaccag acgaccgagg
     1141 cgaccagcgc aaacaggaac ggcgtgtggg caggccgctg ttgaagggcg aagatcaggc
     1201 cgccgatcga cggcacgtcg tcattggccg gcgtggccaa gcggcgctgc ggcggcggtc
     1261 cggcggggcg tcgcgaccga gacagatgct tgggagaggg cgcgcctgcg gcctcggggg
     1321 cgtcggcagg cttggcctct tcctgaggcg cgctgagatt ggccggtgtg ggctcttcgc
     1381 gccgcggctc ctcgcggcgg ggctcttcgc gcttgggctc cacgcgcttg cgctgttcgg
     1441 cctggagccg gtcggcgagg gtcgatcgcc gcggtagggt cgacatgcgc tcaaggtcgt
     1501 cggatttgcc cttggtcttg ccctgcatca tcgcttccag ggggcctttg acctcttcct
     1561 tgtcgtcgga ggtctctttc cctggggccg atttcgcttg tttctgcgcc attacgcgtt
     1621 cctcgcacca ctaaatagaa ctgggaaaac agtgcgccct agaactcaaa acccgcaaca
     1681 gcggcgcatc gcatctcgtg ggaccccgca tcatcttccg aggaagtcta accggaagag
     1741 cccccaacgc tttttcccct cgcccaacat cgttaacgtt tttctaggct taaaaaaagc
     1801 tgaatcgcct gcttgggtta acgccggtta actccctagg attgaacccc aattttgctg
     1861 cgaggcaaag gcgaatcggc taagtggagc ctgggaccgt tgccaggtgc gagggtttcg
     1921 tcactttgcg cagggccggc cgaaaataga gccgtttcaa gggttcttga ggcagcgggt
     1981 ttggggcggc atcgagcgag gcgcatggcc agcaagacgg cagcagaagt ccaagcttcg
     2041 acccctgcca acgaacaggg cccctcgggc aatcccgtgg attttgagca cttggcgcgc
     2101 tacacgctcg gcgagccgga gcttgagcgg gaggttctgg ggctgttctg cagccagtcc
     2161 gttctccttc tggatcagct ccgcgcggcc aagtccgatg aggactggaa gcaggccgcc
     2221 cattccctca aaggttccgc ccgcgcgatt ggcgcctggc gcatggcgca agccgcagaa
     2281 caggccgaag cccttcaagg cgatggtctg agcgagctcc gcagcgcgcg ggtcgatgag
     2341 gtcgaagcct cgctgcgtga ggccgaggcc tatatcgcca cggtgcttga gagccgctga
     2401 ccggcatctt tcgccacaag gggccgcaag gctctggtgt ctcaaattca attgccttat
     2461 agagcacggg ctgcgggcgg gcatgcccca accgcgccaa cccttcgggc gcggtcaccc
     2521 cgcaagacgc ccggctcccg agataaggat tttgcgcgtt agagcccgcc aacgtcatgg
     2581 ccaggatcac atacattcaa cccgacggaa ccgagaacgt tgtcgatgcc gatcccggca
     2641 tgaccgtgat ggaggccgcg gtgaagaact cggtcagagg cattgcggcg gaatgcggcg
     2701 gtgcctgcgc ctgcgcgacg tgccatgtct acgtagagga cgcgtggcgc gagccgacag
     2761 gccagccgga gcagatggaa gaggacatgc tcgactttgc tttcgatatt cgcccggaga
     2821 gccgcctgtc ttgccagatc aaagtgaccg aggagctcga tgggctcagg gtccgaattc
     2881 ccgaaaaaca gttctgaggc gctatgtcag cggaagtgat caagaccgac gcggttatca
     2941 ttggggcagg cccggtgggc ctctttgccg tgttcgagct cggccttgtc gacgtgaagg
     3001 ctcacgtgat cgacatcctc gaccggccgg gcggccagtg cgccgagctt taccccgaaa
     3061 agccgatcta cgatgtgccg gctctgcccg tcgtcaccgg tcaggagctg accgacaagc
     3121 tcatggagca gatcaagccc ttcggcgctg agttccattt cagcgagcgc gtcgaaacgc
     3181 tcgagcgaac ggacgagggc ttccacctcg tgaccgatgc cgacaccgcg ttcgagtgca
     3241 aggtcgtggt gattgccgcc ggcggcgggt ccttcacgcc caagcggccg ccgctcccgg
     3301 gtatcgaggc gtatgagggg acgtccgtct actactcggt gcgcaagatg gatgtgttca
     3361 aggacaagga cgtgcttatc gtcggcggtg gcgactctgc gctcgattgg acgctgaacc
     3421 tacagccggt cgccaagtcg ctgacgctgc tgcatcgacg ctcggagttt cgtgcggcgc
     3481 cggcctcggt gcagaagatg ctggatctgg tcgaaaacgg ggatgccgcc ttcaagctcg
     3541 gtcaggtgac ggagctgcac ggggaccacg gccaactcac cggcgtcacg gtcaaagggc
     3601 ccgacggcgc gacgttcgag caatcggcgg atgtcatgtt gccctttttc ggcctgacca
     3661 tgaagctcgg cccggtggcc gattggggtc tcaatctcga agagaactta atcccggtcg
     3721 atacggagaa gttcgagacg agcgagacgg gcatcttcgc catcggcgac atcaacacat
     3781 acccgggcaa gctgaagctg attctgtcag gcttccatga agccgcgctg atggcgcagc
     3841 aggtgcacaa aattgtgtac cccgacaaga agctgatctt ccagtacacg acgtcgtcgt
     3901 cgaatcttca aaagaagctg ggcgtgaaat aggctggccc ttaggcctaa ttagctcagg
     3961 ccaacgtgcg gtagatcgcg acgtcgtagg ggctgaacag cctgatcttg aggcgctcgt
     4021 tcgccggaat ttccacacgc gcaaacacgc cgctctcatc cagaaggtcg gtcgcgatcg
     4081 ggtcgacgag gacgaggtag agacgccgcg gcttgtcgcg gtacgacacg acgatgttgt
     4141 tcaagacctc ggcgaaggct tcgcgcgaaa acggattgaa aaagtagtgg atcgcttcgc
     4201 cctcgggcgg gccgacctcg gtcgcatcgc caagcacgca ctccacgtta cggcagcgca
     4261 tatgggagcg cgggtattgc gcgatgttca ttgcggcgtc gtcgtgcagt tcctcggcga
     4321 actcgatgcc gccgacggcc gcaaacgggt gctcggctgc gagtaacagc acgcgtccct
     4381 tgccggcgcc gtgatcgacg aaggtaagct tcgagaaatc gtggtcgatg cctgcaaggg
     4441 tccattggaa caattgacac ggtgtcgggc ggtagtcgtg tccgcgctcg ggatgacggc
     4501 tgcggatcgt gagtcccgat agcgctactg gatctttggt cgaggagaag aacaactgct
     4561 ctttcggcgg atcgaaaacc cgtgcgttca gccagtgcaa aaacgctttg tagtcttgct
     4621 tgatccgctc gggacgcgcg tgctccttaa tcttcgcgcc ggtccgttcg atctcgggca
     4681 gcacgctccc tcgtccatac tcggccgcgc gagccaagcc gtctttggtg gcctgcttcg
     4741 tctgactgga ggtcttgtcg gcgagttggg caaggcgccc ggcaaggcgg ttgcgcagtg
     4801 cgccgcggcc caaggcggcg gcattcgtat ttgagtcctt ggtcctcgac atggcgccta
     4861 cagccgccgt cttggtatgg ccgcccgacg aagtaccacc ttgcggcgca gtccggcctg
     4921 gaccgcgggg ggcgaccgtc atgggagttc cccgttcatg cgtaaggatt gtgcacgatg
     4981 cctcgtgaag caagcgcttt ttaggcaaag gccggctccg caacgcaaac gcgtctgccg
     5041 aatgtgctat cctcttgatt cgcgaggggg acccgcgacg gccggcacaa accccaagcg
     5101 gcggggagcg aggcgtgcag gcagaagact tccatgactc cgtgaggcca ggcctcattg
     5161 ccatgttgcc gaggctcatg cgtttcgccg acgtgcttgt cggcgcgcgt gacgaaggcg
     5221 cagggctgct gagccgcgcg ctccagctca tgctggctga gcagcatcgc taccagcgcg
     5281 gcacgtccct cgatgtttgg gccttcgccg agatctaccg gtattggctg agcgagttgc
     5341 gcgatcatgc cgagcccgtt gcgcaagcca ggatcgacga gacgcggttt gtcgagttgt
     5401 tctacgccaa aggcgacgac tacgttgacg cgtcggtcgt tgccttcctg gggagcctgt
     5461 cggcgccaca acgcctcaca gtgctcctcg tctatggcga aggctttgag tacgaggacg
     5521 ctagccgcat cctcgacgtt ccacccgaga caatcgccgc acggctcatc cgtatcagtg
     5581 cggtgcttgc ggaccgcttg aacgcacggg cgccggcgca agccgcggcg accgtcgaga
     5641 cgctctatcc cgaagggctc aggggctgac catgactgaa ctcagcgacg aactcctcat
     5701 cgcctatgtc gatggacaac ttgcccgcaa acagacgcgt gcggtggagg gggttctcga
     5761 tcaagacgat gttcttgcgg cgcgggtcga cgcgctgaag cacgcgcatg cgcgtctgga
     5821 agcggccttt gaggctatcc tggccggcga agagatggac atctccgaag tgccggtccc
     5881 gcaagacgac ggcatcttcg tttcgtggcg caccataaaa atcgccacgg ctggtctcgg
     5941 cctggccgtg gccgctgcgc ttgccgtcgc cgggtacggg tgacctctca aacttccggg
     6001 ccaaggtgcg gaagtgcaga tcgatgtccc cgcgcagacc gcgccccagc cgcaaacgtc
     6061 aacgggtgcc aaccgctaac gcgccatgcg cgcgatcgcc ctgtaatcgc cggagagatg
     6121 gcggttgagg tcgacccgga tcgcgtctag ccgatcctta gtgcaatagc cctgatcgtt
     6181 tttgatctgt tggaacacgg actcgagcgt gtagtcgtag gcatgtgtgg tcttcgccat
     6241 tcgtgcctgg ccatggccaa aacgcgattc ataggcgagg tagttcgagc ggagctgctg
     6301 ggggttgaag acgaacccgc agcgggttgc gcgggccgag gtccatgcga cctgggttgg
     6361 ccgcgcgagc gggtcgttgg gccgcacatt gtatgccgcg gcctcggtgg acgctccggt
     6421 ctccggacct ctgatgctgt ccaccgtcga gcaggccgtc agcgcgacca acagcgcggc
     6481 tgcggcagcc gctgcaatcg gcgtggcggt tgagaagggg tgtttcattt cggcgcgatc
     6541 ttatcggtcg cgtcgtcgca gctaaagggg gcgaaatgaa gtgcaaaaac aaggaaaggc
     6601 ggagctttgc ggccccgcct ccctgttgcg atgtcgagcc gcacgcccgt tcgggacgat
     6661 tggtcggcgc cgcgagacct tatcggcctc ttggtcacgc gcctccctca tggtctccca
     6721 caagcagcgc cgcgacctga ccacggccct tattttgcgt cgcgttgcta cgacgtggca
     6781 ccagccccgg ccgggctgaa accaatcagc gatgggatgt gtggcgcaaa ggaccctgtc
     6841 gacctcgact cacggtaatg cgacgacgtc gccagaccgt ggaccatttg cgtctacgct
     6901 tcccgccgcc ttcagccgcc catggcaatg gacgacctcg ggcttgccat gccctgcgag
     6961 ccttccgatc atttcccgaa gctttgggat ttctccgtca gccccttcta caaccggcca
     7021 actcacagtt tgaagaatac cgcaatgcat tcgcgttgca ctggtggcgc accgcttttc
     7081 cccgcaatcc accggatcgc acgcccccgg ccacgttgcg gccgcccgca aggctttggg
     7141 aagagcgccg tttcgtcccg ttggaccgcc gtttttctga gctgtatgct aggctccaag
     7201 tcgggggagt tcagtttcat gcggcgtgtc gcagtaagag cagtatcggt cttgccgggc
     7261 cttgtcggta cccgcgcgcg gcggctccta ctggcggctg gcgcggcggt gctcgggggg
     7321 cttctggcgc tcggcgccga tgtctccggg ccaggcggcg cctcgctgga catgagaaat
     7381 ctcgtcggcg gggagaccgt cgtttccaag cacgcaaaga ccaaagggcc ggctgcggca
     7441 tcggccgcag acggcttcga gccaacgagt gtcagccggt tgttgaccat ggcgctggcg
     7501 ccgccgcttc ccagccctgc acaagacgcc gagccgaacc tcttggggct aagcgaaatt
     7561 cccagagagc tgatctggaa ccggccgcca aaaaaggacg acgggaagaa gcgcagagcc
     7621 ttcgccgctt tctccaaggc cagcgcaaat cttccctggg acgctgtgga gccggtcccg
     7681 ttcagcccgc tcggccccac cggaaagtcg gcgcagaaaa agacgtcacc gaacggcgct
     7741 gcaccggtgg cgcaagccgc cgtcgtgctg ccggcaggcg gtgaggtgaa gcagtggatc
     7801 aagtccaagg tcaccgagat caagggctcg gcgcgctcgc ggccgcttta tcacttcgag
     7861 ctttggttgg agcctcccgc cgagatgaag cagcgcctgg ttggcgtctc ttacgcattc
     7921 aactcgccgg ccatccgccc gcagttgcaa gcctcgagcg acaagacgaa cggcttccgc
     7981 atcagcgcgg gcggacttgc ttgcgccgac gaggtcaagc tgacgctcag attcaacgac
     8041 ggcggctccc aaaccgtcgc gctcgatggc tgcaatctcc tgagttgatg catcacttga
     8101 tgccggcgac ctcggcgacc agttcgcgga agcgcgggcg cggctgctcg atatagggca
     8161 gggggcggca aacatcgatc gccgcgatgc cgatgcgcgt ggtcagcgct ccattgaaca
     8221 cgccttcgcc gagccgtgcc gaaaggcgcg cagtcagtcc gtgtccgatc acctgctgga
     8281 tcacgtcgtc gccgagcgca atgccgccgg tgagcacgat atgcgtgacc accatgcgcg
     8341 cgagcttcaa cagcgacaac gtcccggggc gtgcgccata gagcgtggcg agcttgcgga
     8401 gcatgcgcag gttctgcgcg ccgacgaaca tcatgtcgat aagcgcgttg gggctaaccg
     8461 ccgtgaccac cgacacgcgc tttgccgatg tggcgacgat gccgcgtgcg acgggatcga
     8521 gcggcgcgat gagtgtgcgc tcgtcgagac gcagcagctc ctccgcgttc atgatgtcat
     8581 gctcgtgctc ggctagacgc tgcaagctcc aagcaaggtc gtcgcgtccc gcatagaaac
     8641 gcttcagccg tcgcgtcacg tcgacggcga gcgccttgtc gttctggcgc gccgcgctgt
     8701 ccgcctcgtg gcggattttg tcgaggcgtc caagccgcaa cagcccggcc acctcgcgga
     8761 tgatgatcat gagaagtgcc agcacgacga cggctagcag cccgacagcg acccagccga
     8821 tccagtcgtc ccgcgccagg agcgaaagca cccaatcgta gagccatagc gaggcgatga
     8881 ggctgatcag cccgccgagt gcggcgagaa agatgctgcc ccagcgtaag ccgcgatcca
     8941 gattgggcag catgcgcgtt tgcgcgtcca taggagtagg gagcgtttcg gcggccggcg
     9001 gctccggcgg cacgaacacg tccacgtctt cgacgcggaa cgccgcgggc ttccggcgct
     9061 tgggtgcggt catgcgaggc ggtctccgat cagggtctgg atggcgcggt cgagccggat
     9121 atttgggaac ggcgcgacgc ctccggaagg cagctgcacc ggattgggcg gccgaaagcg
     9181 aacgaagcgc atattgccgt gctcggtctg ccagccggac agcgctttct tcggatcctc
     9241 gggaagatcg cctgggaaga tggcgaaggg ctcgttgccg tcgaagatac gccggccgat
     9301 cttctcgccc tttagcggat agccgacgat gcacggcaac gtctgggtgc cccgcttcgc
     9361 ttcgccttct tgcgtggcgc ggatggcggc cagcgccaga acgttgacgt cggcgccgcg
     9421 atagcgggcg cgggccagcg ccttgtccgt gagcagcgac aggatcgcct ccaaccggtc
     9481 atgactctcg tggtgcagat ggtcggcctt cgtcgcggca agggcaatgc ggtcgatccg
     9541 cggtgcgaac aggttcgacc agagattgtt tgcgcccgtg cggaagcatt cgaggatctc
     9601 ggtcatggcg cgttccagat cggcaactgc cgccggcccg ccgttcaacg cggcaagcac
     9661 gtcgatcaac acgacctgcc ggtccagccg cgcaaaatgg tcgcggaaga aggggcgcac
     9721 gacgtaggtc ttataggctt cgtagcgccg ctccatgagc gaccacagcg agccgcgcgg
     9781 gaaactgtcg gcacccttgg catcgagcgg cgcgaaggtc agcgcaggcg agcccgcgag
     9841 atcgcctggc ataagaaagc gtcccggcgg caaggtggac agcgcatatt ggtcggagcg
     9901 cgcctcgcgc aaataagtct tgaacagatc tgcgagcttc gcggcgagcg cttcgtcggc
     9961 aggctcagcc ggatcgatct cgccgagcgc ggtgttccaa tccttggccg ccgcttcccg
    10021 gtccgcttcg cgcgcctgcg caaccgcgtc cctagcccaa ctggcatagt cgcgttgcag
    10081 gagcggcaga tcgagaagcc attcgcctgg atagtcgacg atgtcgatat gcagccgctc
    10141 gcgccccaat tgccgtttcc agaaccgcgt cgaggcgtat tcgagcgtca ggcgcagctg
    10201 gctgatctgg cgtgtgctct ccggccaggt cggcggctcg gcggtgaggt cggcgagatg
    10261 tctttcgtat tcgaagcgcg gcacgatctc gtcgggttgg ggctcgagat agacgcgtat
    10321 caagcgtccc tccgcgcccg catcgaagaa tggcagccgc ccactggcga tgagattatg
    10381 gaccagcgcc gtgatgaaca ccgtcttgcc ggagcgggcg aggccggtca cgccgaggcg
    10441 caatcgcggc gttgcgaggc cggaggcaac gtctcccacg ttgcgtacgg cgtcgcccgc
    10501 ggcctgggta taggtggaaa gtggcacgct tcagatcagt ccttttttct cggccccccg
    10561 tgccatgcgc ctcatagcgt gctcggggca acgaaggaag accagcggga actcttggta
    10621 cctttttgac tgtgcacctg cacaacaaat gtgcgccgca agtcccagcg ctgcctatga
    10681 gatatgacga ttccgcgatg ttctcgggag cggcgcttgg cgcatttgtc aaccccgcgt
    10741 gttgtcttga cttgtcaact cgcttccgta aatacggggc tgcctggtgg gggaacgctg
    10801 aggcgtgccg ggtgaagtta agtaggcgac gagccgatga agtggatggc ctggacgtga
    10861 ctctcatggc gactgcaaga aagaagaaga aagtcctccc cgaggattat cggccgtcgg
    10921 atgatgagcc gttcatgaac gagcagcagc gggcctattt ccgccgcaag cttctcaact
    10981 ggcgtgacga gattctgcgc tccaccaagg agacgctgca gcatctgcag gacgactccg
    11041 agcaatatgc cgacattgcc gaccgcgcga cctccgagac ggatcgctcg ctggaactgc
    11101 gcgcccgtga tcgccagcgc aagctcatcg ccaagatcga tgacgcgctg gagcgaatcg
    11161 agggcggtac gtacggctat tgcgaggaaa ctggcgagcc catcagcctc aaacggctcg
    11221 atgcgcgtcc catcgcgacc ttaagcgtcg aggcgcaaga gcgtcacgag cgccgcgagc
    11281 gcgtctaccg cgacgtgtag cgcgcgacgg tctttcgaga ccggtagccc aacatcgcta
    11341 atgcgccgcc gacagcggct tcgccacatc ttcgagcgat ttgccttcgg cggcgacacc
    11401 gtagcgtgcc gcgataacgc ttgcggccag catcaagccc gcgcccagca ggtacccggc
    11461 gaacaggctc atgcgcgaac ccgtatcgat cagcgccccg aacagccacg gcgcgccgat
    11521 gcccccgaca cccgtcccga cggcatagaa gaaggc
//
LOCUS       DBNHVU010000055         3929 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1209424, whole genome shotgun sequence.
ACCESSION   DBNHVU010000055 DBNHVU010000000
VERSION     DBNHVU010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1209424"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="AC16Y7_02155"
     CDS             <1..927
                     /locus_tag="AC16Y7_02155"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR5708212.1"
                     /translation="IQKKAKNMLVRVDGKLPEGVTAKDIILAIIGEIGTAGGTGHVIE
                     YAGEAIRDLSIEGRMTVCNMSIEGGARAGLIAPDEKTFAYIEGRPRTPKGGAFEVAKA
                     HWEALYSDEGANFDAEVVLRAENLPPIVSWGTSPEQVVSVAGRVPVPSEIDDPTKRKA
                     AERALDYMGLKGGEKVTDIALDRIFIGSCTNGRIEDLRAVAKIVEGQHVNENLNAMIV
                     PGSGLVKLQAEEEGLDTIFKAAGFEWREPGCSMCLAMNPDKLSPGERCASTSNRNFEG
                     RQGRLGRTHLVSPQMAAAAAIAGRFVDVRETS"
     gene            953..1723
                     /locus_tag="AC16Y7_02160"
     CDS             953..1723
                     /locus_tag="AC16Y7_02160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5708213.1"
                     /translation="MTSLSQKKFGEAAADYAGSSTHAEGPSLARLAELIAPEPTWRHL
                     DVGTGAGHTALAFADQVAKVTASDITPEMLQEVRKAAEKRGLGNVATRQANAQDLPFP
                     DTSFHLVTCRLAAHHFANPRSFVAEAARVMIPGGTFAFVDNISPSDEALASAYNAFES
                     LRDPRHGRCLSLAEWSTLIEDAGLTLTSAEVLDKDIAFDPWVARMRCTDAVIARLKEI
                     LTGEPLRELLHPRETTDGLTFTLREAIVIAQKPAEAHR"
     gene            1720..2127
                     /locus_tag="AC16Y7_02165"
     CDS             1720..2127
                     /locus_tag="AC16Y7_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HIR5708214.1"
                     /translation="MIDLNKHRAPSLTEFETIANTAFKRLPAAFRDMTKDVLIRIEEF
                     PTDEVLDSLGIDSPFGLLGLYQGVDLTRQSILDVSPLPEMIFLYRRPILDYWASHEEK
                     LGDIITHVLVHEIGHHFGLSDDDMERIESEATA"
     gene            2258..2878
                     /locus_tag="AC16Y7_02170"
     CDS             2258..2878
                     /locus_tag="AC16Y7_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708215.1"
                     /translation="MFPRRAIYQFSAAFGLALAVFASGGSPVRAQEFVYRGNSATGPT
                     VAQYLEQIGPAGKEIPHGQLKLDGKSVNCGKRPTVLNPNFDSWGGAFPGFLILNTKKI
                     KGLSTQVKLYIYSHECGHQFIGANETKADLFAIRRGVKWGWLNAQGLEEICIFISKLK
                     GDAVHPPGTQRCETMRTYYRQLVAGNDQHRAGTSSNWRPTTLGPSN"
     gene            complement(2912..3235)
                     /locus_tag="AC16Y7_02175"
     CDS             complement(2912..3235)
                     /locus_tag="AC16Y7_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708216.1"
                     /translation="MAGEGDRQDRKEATKLFIAGALALILLVAMGAAGLYYATNMMGE
                     ADPFSEGLGLKNAAVISFAVSFVAILVMTVASGGDAILGELPFTIVGFLIFFVVFWLM
                     IAWIF"
     gene            complement(3248..3643)
                     /locus_tag="AC16Y7_02180"
     CDS             complement(3248..3643)
                     /locus_tag="AC16Y7_02180"
                     /inference="COORDINATES: protein motif:HMM:NF015648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR5708217.1"
                     /translation="MAGEVIYEAHPAMFRAHPFWFILFVLLIAAFGIGLFILLYWYIT
                     TRATALSVTEHSLLYERGILSKERLAVSLRHVRSVQVSQGFINRILGVGTIEIKTAGD
                     EPEFTVDDLPDPHQVREAISRAQAMAGDN"
     gene            3764..>3929
                     /locus_tag="AC16Y7_02185"
     CDS             3764..>3929
                     /locus_tag="AC16Y7_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR5708218.1"
                     /translation="MEKFTTFTGIAAHLPMINVDTDMIIPKQYLKTIKRTGLGEGLFA
                     ELRYDEAGEKL"
BASE COUNT          815 a         1295 c         1163 g          656 t
ORIGIN      
        1 atccagaaga aagccaagaa catgctggtg cgcgtcgacg gcaaactgcc cgaaggcgtc
       61 accgccaagg acatcatcct cgccatcatc ggcgagatcg gcacggctgg cggcactggg
      121 cacgtcatcg aatatgccgg cgaggccatc cgtgacctct cgatcgaagg gcgcatgacc
      181 gtctgcaaca tgtcgatcga gggcggcgcc cgcgccgggc tcatcgcacc cgacgaaaag
      241 accttcgcct atatcgaagg ccggccgcgc acgcccaaag gcggcgcctt cgaggtagcg
      301 aaagcacact gggaggcgct ttactccgat gagggcgcca acttcgatgc cgaagtggta
      361 ctgcgcgccg agaacctgcc gcctatcgtg tcttggggaa caagcccgga gcaagtcgtc
      421 tccgttgccg gccgcgttcc ggttccctcc gagatcgacg acccaaccaa gcgtaaagcc
      481 gcggagcgcg cgcttgacta tatgggcctg aagggcggtg aaaaggtcac cgacattgcg
      541 ctggaccgca tcttcatcgg ctcatgcacc aatggccgta tcgaagactt gcgcgccgtg
      601 gcgaaaatcg tcgaaggcca gcacgtcaat gagaacctga acgccatgat cgtgccgggg
      661 tcgggtctgg tgaagctcca agccgaggaa gaaggtctcg acacgatctt caaagcggcg
      721 ggctttgagt ggcgcgagcc gggttgctcc atgtgtcttg ccatgaaccc cgacaagctc
      781 agccctggcg aacgctgtgc gtccacctcg aaccgcaact tcgaaggccg ccagggccgc
      841 cttggccgca cgcatctggt gtcgccgcaa atggccgcgg ccgccgcgat tgccggacgc
      901 ttcgtcgacg ttcgcgagac gtcctagccg atccccgtcg ctgggagctc acgtgacgtc
      961 cctgtctcaa aaaaagttcg gagaggccgc cgccgactac gcaggctcgt ccacgcatgc
     1021 ggaaggtcct tcgctcgccc ggctcgccga actcatcgca ccggagccca cctggcgaca
     1081 tctcgacgtc gggacaggcg ccggccacac tgcgctcgcc ttcgccgacc aagtggccaa
     1141 ggtcaccgcg agcgacatca cgccggaaat gcttcaggag gtccggaagg ccgccgagaa
     1201 acgcggcctc ggcaatgtgg cgacgcggca agcgaacgcc caagaccttc ccttccccga
     1261 caccagcttc cacttggtga cctgccggct cgccgcgcat cacttcgcca atccgagaag
     1321 cttcgttgcg gaggccgcgc gggtgatgat ccccggcggc acgttcgcgt ttgtcgacaa
     1381 catctcgccg agcgacgagg cgctggcctc cgcctacaac gcctttgaga gcttgcgcga
     1441 tccgcgccac ggccgctgcc tcagcctcgc cgagtggagc acgctgatcg aggatgcggg
     1501 gctgacgctt acctcggccg aagtgctgga caaggacatc gccttcgatc cgtgggttgc
     1561 gcgcatgcgc tgcaccgatg ccgtgatcgc gcgactgaag gagatactga ccggagagcc
     1621 gttgcgggag ctcttgcacc cacgcgagac gacggacggg ctgaccttca ctttgcgtga
     1681 agcgatcgtg atcgcgcaga agccggcaga ggcccatcga tgatcgatct gaacaagcat
     1741 cgcgcgccga gcctcaccga gttcgagacc atcgccaata ccgcattcaa gcgcctgccg
     1801 gcggcattcc gcgacatgac caaggatgtc ctcattcgca tcgaggagtt ccccaccgac
     1861 gaggtcctgg actcccttgg catcgactcg cccttcgggc tgctcggcct ctaccaaggt
     1921 gtcgacctta cgcggcagag cattctcgac gtttcgcctt tgccagagat gatttttctc
     1981 tatcgccgcc ccatcctcga ctattgggca tcgcacgagg aaaagctggg cgacatcatc
     2041 acccacgtgc tggtgcacga gatcggccac cattttgggt tgtcggacga cgacatggag
     2101 cgcatcgaat ccgaggccac agcctagccc aaagtctgcc ggaatttgca ggcttcctcc
     2161 acactgaggc cggaaggcaa caggtgacgc gccttgagca acagatgccg cgcggtaatt
     2221 gcgcggatac ctttcggcgc atagagtagg acccatcatg tttcccaggc gggcgatcta
     2281 ccagttttct gcggctttcg gcctcgcgct tgcggtcttt gcgagcggcg gctcacctgt
     2341 gcgcgcgcag gagttcgtct accgcggcaa cagcgcaacc ggccccaccg tcgcacaata
     2401 tttggagcaa atcgggcccg ccggaaaaga gattccccac ggccagctca agctcgacgg
     2461 caagtccgtc aattgcggca agcggccgac ggtgctgaac cccaatttcg actcctgggg
     2521 cggcgccttc ccgggcttcc tcattctcaa caccaagaag atcaaagggc tctccaccca
     2581 ggtgaagctc tacatctact cccacgaatg cggtcaccag ttcatcggcg ctaacgagac
     2641 gaaggccgac ttgttcgcca tccggcgtgg cgtcaaatgg ggctggctga acgcgcaagg
     2701 gctcgaggag atctgcatct ttatctccaa gctcaaaggt gacgcggtgc acccgcctgg
     2761 aacgcagcga tgcgagacga tgcgcaccta ttatcgtcag ctggtcgccg gcaacgatca
     2821 gcaccgggcc ggcaccagtt ccaattggcg gcccacgaca ctgggtccgt ccaactagcg
     2881 gaacgcttcg cggcgaaagc cgcacggacg cctagaagat ccaggcgatc atcaaccaga
     2941 acaccacaaa aaagatcagg aagccgacaa tggtgaaggg cagctcgcct aaaatggcat
     3001 cgccacccga ggccacggtc atcaccagga tcgccacgaa ggacaccgcg aacgaaatga
     3061 ccgcggcgtt cttcagcccg agaccttcgg agaagggatc cgcctcgccc atcatattgg
     3121 tggcgtagta gagcccggcc gcacccatcg ccaccaagag gatcagcgcc agcgcgccgg
     3181 cgatgaagag cttggtcgcc tctttccggt cttgccggtc tccctcgcct gccatgcagc
     3241 ctccgtccta attgtccccg gccatggcct gagcccggct gatcgcctcg cgcacctggt
     3301 gcggatcggg caagtcgtcg acggtgaact caggctcgtc gcccgccgtt ttgatctcga
     3361 tcgtgccgac gccgagaatg cggttgatga aaccctggct cacctgcacc gagcgcacat
     3421 gccgcagcga caccgccagc cgctccttgc tcaaaatgcc ccgctcgtag aggagcgagt
     3481 gctccgtcac cgaaagcgcc gtggcgcggg tcgtgatgta ccaatacagc aaaatgaaga
     3541 ggccgatccc gaacgcggca atcaggagca cgaacaaaat gaaccagaaa ggatgcgccc
     3601 ggaacatcgc cggatgcgcc tcgtagataa cctctcccgc cattctgttg cctcccgttg
     3661 ggctgaggac cttaagccgc accatggcct gagaaaagcg cggatatgtg acggtggaaa
     3721 cgcgggccct gagccgctat atggctcaca taagaggaca gcgatggaaa aattcacaac
     3781 gttcacgggc atcgccgccc acctgccgat gatcaatgtc gacaccgaca tgatcatccc
     3841 caagcagtac ctgaagacga tcaagcgcac cggccttggc gaaggcctgt tcgctgagtt
     3901 gcgctacgac gaggctggcg agaagctgc
//
LOCUS       DBNHVU010000056         9641 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1803023, whole genome shotgun sequence.
ACCESSION   DBNHVU010000056 DBNHVU010000000
VERSION     DBNHVU010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 9641)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9641)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9641
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1803023"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..1405
                     /locus_tag="AC16Y7_02190"
     CDS             197..1405
                     /locus_tag="AC16Y7_02190"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR5708219.1"
                     /translation="MTDIKKVVLAYSGGLDTSIILKWLQETYGCEVVTFTADLGQGEE
                     LEPARKKAEMLGIKEIFVEDLREEFVRDYVFPMFRANALYEGVYLLGTSIARPLIAKR
                     QIEIAKETDADAVCHGATGKGNDQVRFELSYYALNPDIKVVAPWREWDFVSRSDLIEF
                     AEKHQIPVAKDKRGEAPFSVDANLLHSSSEGKVLEDPNAEPPPYVYQRTIAPEDAPDT
                     PTVVTVGFRNGDPVSIDGNAMSPATLLTKLNELGHDNGIGRVDLVENRFVGMKSRGVY
                     ETPGGTILLAAHRAMESLTLDREAAHLKDSLMPRYAELVYYGFWFAPEREMLQALVDK
                     SQENVEGEVTLKLYKGNVIVIGRKSDKSLYSTDIVTFEDDAGAYDQKDAEGFIKLNAL
                     RLRLVGKRRR"
     gene            2182..3297
                     /locus_tag="AC16Y7_02195"
     CDS             2182..3297
                     /locus_tag="AC16Y7_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR5708220.1"
                     /translation="MRWSLNLGSIAGTAIRIHVTFLLLLVWLGAIFYRQGGPEAAWHG
                     TIFVVLIFVCVLLHELGHVFAARRYGVRTRDITLWPFGGISSMERMPDKPSQELIVAV
                     AGPAVNVAIAAVLILWLGPRFNLEALTEIENPAVSLAVKIAGANIILVVFNMIPAFPM
                     DGGRVLRALLAMRMGNARATQVAATIGQGFAVVFGVLGIFYNPMLIIIAAFIFLAASG
                     EATQAQLRAVAQGTLVSDAMVTKFESLPTSATVADAADALIRTTQTEFPIVDGTGHLR
                     GVLTRAAMIKALKDRGPDTPVLEVMADVPTVHANTKLDVALRALMQRGQPVIGVTDTK
                     DKLVGLLTVENLGEMMMIHSARPETGSEPGPWSNMGR"
     gene            3385..5211
                     /gene="typA"
                     /locus_tag="AC16Y7_02200"
     CDS             3385..5211
                     /gene="typA"
                     /locus_tag="AC16Y7_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR5708221.1"
                     /translation="MKLRNIAIIAHVDHGKTTLVDRLLQQSGAVRDNQRVDERAMDSN
                     DLERERGITILAKATSVIWNETRINIVDTPGHADFGGEVERILNMVDGALVLVDAAEG
                     PLPQTKFVVSKALKMGLKPIVLINKVDRADARPDEVLDEVFDLFAALNASDEQLDFPI
                     LYGSAKEGWIADSPEGPKDQGMLPLFELVLRHVAPPSIEDGPFRMLGTILEANPYLGR
                     IVTGRITSGTIKPNEMVKVLDHEGGVVEQGRVSKVLAFRGLERTPLDEASAGDIVSIA
                     GLPNATVAHTMCAPTVDTPLPAQPIDPPTLSMTFRVNDSPLAGTEGSKVTGRMIRDRL
                     LREAEGNVALRVTESEERDSMEVAGRGELQLGILIETMRREGFEISVSRPKVLLTSGE
                     GGTLMEPIEEVVIDLDEEHSGAVVQKLAERKADLIEMKPSGGGRQRLVFHAPTRGLIG
                     YQGELLTDTRGTAIMNRLFHGYAPHKGVIQGRRNGVLISTDQGEAVAYALWNLEDRGP
                     MMIDPGARVYRGMIVGEHTRGNDLEVNVLKGKKLTNIRTTSKDEAVRLTTPLRMALER
                     ALAYIENDELVEVTPKSIRLRKRHLDPNERKKAERQRAEEMA"
     gene            5297..5803
                     /locus_tag="AC16Y7_02205"
     CDS             5297..5803
                     /locus_tag="AC16Y7_02205"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5708222.1"
                     /translation="MSVDVAHIRPARPKDATALVAAYEEAWRGAYQGIIPHLSLERMI
                     ARRSLRWWNNAIGKRAPILVLDFQGEAAGYTTFGRCRLGSLPHRGEIFELYLHPTYQG
                     LGLGQKLFAGARHRLGELRLKGLLVWALADNEAACGFYFSLGGKPVAEGSESFGDTAL
                     RKVAFAWG"
     gene            5899..6492
                     /locus_tag="AC16Y7_02210"
     CDS             5899..6492
                     /locus_tag="AC16Y7_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR5708223.1"
                     /translation="MTDVGARDRWIMAGLVMLGLFTVLAGVLSGRSTINYVLGRDARD
                     ASLTWAKEVNDRLRTADHSDLKVLDTAAFHSIVNDGSSTGLTLPTGATFADDGFKLID
                     GFNRLTQGWLLGTVNTDSSEFVSQREGFAILAPTGKALAAEGVETESQATMLKEWGCD
                     QVQGYYYGGPETEIPDDKADDTPLDMESRPLDRPLVA"
     gene            6657..7454
                     /gene="dapB"
                     /locus_tag="AC16Y7_02215"
     CDS             6657..7454
                     /gene="dapB"
                     /locus_tag="AC16Y7_02215"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR5708224.1"
                     /translation="MRIAVLGAAGRMGQALTRALAETPGCTVAGGVEVKGSAAIGQDI
                     GAVAGLEPLGHAITDDPLPLFAHIDGVLDFTLPKPTIEYAALAAQARIVHVIGTTGFS
                     DGDLAKLEAAARHATIVKAGNMSMGVNLMAALTEQVARTLGPEFDIEILEMHHRHKRD
                     APSGTSLMLGDAAAKGRNVALGDKSVRTREGDTGPRREGDIGFAALRGGDVVGEHKVI
                     FAGPGERIEIAHIASDRGIFARGAVKAALWGRGKPPGLYSMADVLGL"
     gene            7657..8307
                     /locus_tag="AC16Y7_02220"
     CDS             7657..8307
                     /locus_tag="AC16Y7_02220"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HIR5708225.1"
                     /translation="MLQKACEVPRKSENVLVLVRHGQSEWNRLNMFTGWKDVGLSEEG
                     VSEAHRAGQRLKEEGLHFDSAFASTLRRAHHTLDIILGELGQGKLPTVKAAALNERDY
                     GQLVGIDKEEARKRFGAEQVQIWQRSYDIAPPGGESLKDTAARVCPFFERWIVPELQD
                     GKNVIVVAHGNSLRSLIMELDHLTPEEVKDCSLATATPVIYRLTVDGSVKKTDLAA"
     gene            complement(8304..8810)
                     /locus_tag="AC16Y7_02225"
     CDS             complement(8304..8810)
                     /locus_tag="AC16Y7_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HIR5708226.1"
                     /translation="MAEAKTPSQAPEAQNPQFSAVLTPHRSLGPKGFVIFMAALCVVS
                     FGTGLLFFLMGAWPVLGFFGLDVLLVYIAFKLNFRALRVYETVELGGDALIVTRVDPK
                     GREQSWRFNPYWVRLRLDERVGRNSELSLASHGKRLVFANFLTDPEREDFAAALNAAL
                     RETRSGMA"
     gene            8959..9576
                     /gene="nth"
                     /locus_tag="AC16Y7_02230"
     CDS             8959..9576
                     /gene="nth"
                     /locus_tag="AC16Y7_02230"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR5708227.1"
                     /translation="MFARLAARDPEPKTELRYTDPYTLLVAVVLSAQATDASVNKATP
                     ALFATADTPEKMVALGEKAVTEKIKTIGLFRNKAKNVVALSRKLIEEHGSAVPKSREA
                     LETLPGVGRKTANVVLNVAFGEPTIAIDTHIFRVSNRTGLAPGKTPLKVEETLERVVP
                     ARYKHNCHHWLILHGRYVCKARKPECWRCIIADICVFKPKTPAPA"
BASE COUNT         1901 a         3123 c         2932 g         1685 t
ORIGIN      
        1 caatgcaagc cgcgcgacaa aaccgttttt catgtcatct cctccctgaa gttccccgac
       61 tatcggtgcc ttgaggggcc actataaggc agacctggcc ttgggctcgt tttccgtctc
      121 tcgtcgagct cttgcgcaaa ggttttgcgt gcctatagtc gccgccaagt caactttgaa
      181 cagcccgttt caccccatga cagacataaa gaaagtcgtg cttgcctatt cgggcgggct
      241 cgacacctcg atcatcctca aatggctcca ggagacctac ggctgcgagg tcgtgacctt
      301 cacggccgac cttggccagg gcgaggagct ggagccggcc cggaagaagg ccgagatgct
      361 cggcatcaag gagatcttcg tcgaggatct gcgcgaggaa ttcgtgcgcg actacgtgtt
      421 cccgatgttc cgggccaacg cgctctacga aggtgtctat ctgctcggca cgtcgattgc
      481 ccggccgctc atcgccaaac gccagatcga gatcgccaag gagacggacg ctgatgccgt
      541 ctgtcacggt gccaccggca agggcaacga ccaggtgcgg ttcgagctct cctactacgc
      601 gctgaacccg gacattaaag tcgtggcgcc ttggcgcgaa tgggacttcg tctcacgcag
      661 cgacctgatc gaattcgccg agaagcacca gatccccgtc gccaaggaca agcgcggcga
      721 ggcgccgttc tcggtcgacg cgaacctgtt gcactcgtcc tcggaaggaa aagtgctgga
      781 ggacccgaac gcggagccgc caccttatgt ctaccagcgc accatcgcgc cggaggacgc
      841 gcccgatacc ccgacggtcg tgactgtcgg ctttagaaac ggagaccctg tctctatcga
      901 cggcaatgcc atgtcgcccg caacactgct cacgaagctc aacgaactcg gacacgacaa
      961 cggcatcggc cgtgtcgacc tcgtcgagaa ccgttttgtc ggcatgaagt cgcgcggggt
     1021 ctatgagacg ccgggcggca ccattctcct ggccgcgcat cgcgccatgg agtcgctgac
     1081 gctcgaccgc gaggcggcgc acctgaaaga ctcgctcatg ccgcgctacg ccgagctggt
     1141 ctactatggc ttctggttcg cgccggagcg cgagatgctg caggcgctcg tggacaagag
     1201 ccaggagaac gtcgaaggcg aggtgacgct taagctctac aagggcaatg tcatcgtcat
     1261 cggccgtaag agtgacaagt cgctctattc caccgacatc gtcaccttcg aagacgacgc
     1321 cggcgcttat gaccagaagg acgccgaagg tttcatcaag ctgaacgcac tccgcttgcg
     1381 cctagtcggc aaacgccgcc gctaggactg tgacgcggct ctactttgtg ccgggccttg
     1441 gcacttagcc gacgtctatg acgaagccag agatgtccac acatgaccca aagcggacct
     1501 cagtccagca agtctggact gcccagagtc cgctgagcgt tgaaaagcat gtcgggcgcc
     1561 ttcatgtctg tgaaaaagac cgtggtcggg tccgatgtag ctctggatcg gcccgtgcgc
     1621 cgtagcgcgt gccttggcgt cagcatcgtc agtcatagga agcctcgctt agttggcttc
     1681 cccgaccccg caggccaagg atagaagcct cggcctgtca gcaaccatta tacggtagcg
     1741 gccctaatac ccgtaacgga gcctgtactt gcgacgctct ctaggaggct ttgccgtgag
     1801 atcagcgatg cagttcgtca caaactgttc tcgtccgcct cgaccattaa atgacctctt
     1861 cgacgcctca tggaaacaga ctttctctat ccactctggg gccgcctcac tagttacgct
     1921 tgttatcgac atggctgccg caaaaactat aggtgcgaga tagcgcattg ctttgctcca
     1981 tgtggctggg cttttcttcg gttactgtag tgcgctcgca aggttgtgag ttgttcaatc
     2041 aaaggtgcct tttcggaaag ggcatcattc tgagcgttct gccagcgtcg tcaagcacac
     2101 cgtgcgacag aaaccacctc tatgttcagc caaatgccta tatgctagag gccatctaac
     2161 cggagacacg agccttacag aatgcgctgg tccctgaatc tggggtcgat cgccggcacg
     2221 gcgattcgca tccacgttac tttcctgctg ctcctcgttt ggcttggcgc gattttttac
     2281 cgccagggcg gccccgaggc tgcgtggcac ggtacgatct tcgtcgtgct catcttcgtg
     2341 tgcgtgctct tgcacgagct gggtcatgtg tttgccgcca ggcgctatgg cgtgcggacc
     2401 cgggacataa cgctgtggcc gttcggcggc atctccagca tggagcgcat gcccgacaag
     2461 ccgagccagg agctgatcgt ggccgtggcc ggcccggcgg tgaacgtcgc catcgccgcg
     2521 gtgctgattc tctggctcgg cccgcgtttc aatctggagg ccctgaccga gatcgagaac
     2581 ccggccgtgt cgctcgccgt gaagatcgcc ggcgccaaca tcatcctcgt cgtgttcaac
     2641 atgatcccgg cctttcccat ggacggtggc cgcgtacttc gcgcgctcct tgccatgcgc
     2701 atgggcaatg cccgcgccac acaggtggca gccaccatcg gccagggctt tgccgtcgtc
     2761 ttcggcgtcc tcggcatctt ctacaatccg atgcttatca tcatcgccgc cttcatcttt
     2821 cttgctgcat ccggcgaggc gacccaggcg cagctccgcg ccgtggccca aggcaccctc
     2881 gtgtcggacg ccatggtcac gaaatttgaa tcgctgccaa cctcggccac ggtcgccgac
     2941 gccgccgatg cgctgatccg caccacacag accgagtttc ccatcgtcga cggcaccggc
     3001 cacttgcgcg gcgtgctcac ccgcgctgcc atgatcaagg ccctgaagga ccgcggcccg
     3061 gacacgccgg ttctggaggt gatggccgac gtgccgactg ttcacgcgaa cacaaagctg
     3121 gatgtcgcgc tgcgcgcctt gatgcagcgc ggccaaccgg tgatcggcgt gaccgatacc
     3181 aaagacaagc tcgtcggcct gctcacggtg gagaatctgg gtgagatgat gatgatccat
     3241 tccgcgcggc ccgaaactgg gtccgagccg ggcccctgga gcaatatggg gcgttagccg
     3301 gcaagcccgc attgcggcgc gagggaaaat ccctatattg cgctgcaaca accagccgcc
     3361 acacggccga aagacctcat cagaatgaaa ctccgcaata tcgcgatcat tgcccatgtc
     3421 gatcatggca agacgaccct cgtcgaccgc ctcctccagc agtccggcgc cgtgcgcgac
     3481 aaccagcgcg tggatgagcg cgccatggat tccaacgacc tggagcgcga gcgcggcatc
     3541 accatcctcg ccaaggcgac ctcggtcatc tggaacgaaa cccgcatcaa catcgtcgac
     3601 acgcccggcc acgccgactt cggcggcgag gtggagcgca tcctcaacat ggtggacggc
     3661 gcgctggtgc tggtggatgc cgccgagggc ccgctgccgc agacaaagtt cgtcgtgtcg
     3721 aaggcgctga agatgggcct gaagcccatc gtcctgatca acaaggtcga ccgtgcggac
     3781 gcccgcccgg acgaagtgct cgacgaggtg ttcgacctct tcgccgccct caacgccagc
     3841 gacgagcagc tcgactttcc gatcctctac ggttcggcca aggaaggctg gatcgccgac
     3901 agcccggagg ggcccaaaga ccagggcatg ctgccgttgt ttgagcttgt gctgcgacac
     3961 gtcgcgccgc cctcgatcga ggacggtccc ttccggatgc tcggcaccat tttggaagcc
     4021 aacccctatc ttggccgcat cgtcaccggc cgcatcacct ccggcaccat caagccgaat
     4081 gagatggtga aggtgctcga ccatgagggc ggcgttgtcg aacagggccg cgtgtccaag
     4141 gtcctcgcat tccggggact ggagcgcacg ccgctcgatg aggccagcgc cggcgacatt
     4201 gtctcgatcg ccgggctgcc caacgccacg gtggcgcata cgatgtgcgc gcccacggtg
     4261 gatacgccgc tgccggcgca gccgatcgat ccgccgacgc tgtccatgac cttccgggtc
     4321 aacgactcgc cgctggccgg aaccgagggc tcgaaagtga cggggcgcat gatccgtgac
     4381 cggctcttgc gcgaggcgga aggcaatgtc gcgctgcggg tcaccgaatc cgaagagcgc
     4441 gactccatgg aggtcgccgg acgcggcgag ttgcagctcg gcatccttat cgagaccatg
     4501 cgccgcgaag gtttcgaaat ttcggtatcg cggcccaaag tactgctgac gagcggcgaa
     4561 ggtggcaccc tgatggagcc catcgaagag gtggtcatcg acctcgacga ggagcattcg
     4621 ggcgccgtgg tgcagaagct cgccgagcgc aaagcggatc tcatcgagat gaagccttcg
     4681 ggcggcggac gccagcgtct cgtgtttcat gcaccaaccc gcgggcttat cggctatcag
     4741 ggtgagctgc tcaccgatac gcgcggcacg gcgatcatga accgtctgtt ccacggctac
     4801 gccccgcaca agggggtcat tcaaggacgc cgcaacggcg tgctgatctc cacggaccag
     4861 ggcgaagccg tcgcctatgc gctgtggaac ctggaagatc gcgggccgat gatgatcgac
     4921 cccggcgcac gcgtttatcg cggcatgatc gtcggcgagc acacccgcgg caacgacctc
     4981 gaagtgaacg tgctcaaggg caagaagctc accaacatcc gcactacgtc gaaagacgaa
     5041 gcggtgcgcc tgacgacgcc gctgcgcatg gcgttggaac gcgcgctggc ttacatcgag
     5101 aacgacgagt tggtcgaggt cacgccgaag tcgattcgcc tgcgcaagcg ccacctcgat
     5161 ccgaacgaac gcaagaaggc cgagcgccaa cgcgccgaag aaatggctta gccacaactg
     5221 gcctgacagc gaagaactcg gataacgccg aagctgcctt tgcccggcgc aacagtcctg
     5281 cgttaggctc tagatcatgt ccgtcgatgt cgctcacatc aggccggcac gcccaaagga
     5341 cgcgaccgcg ctcgtcgccg cctacgagga ggcttggcgc ggcgcctatc agggcatcat
     5401 cccgcacctg tctctggagc gcatgatcgc gcggcgtagc ttgcgctggt ggaacaacgc
     5461 gatcggcaag cgtgcgccga ttctcgtgct cgatttccaa ggcgaagcgg ccggatatac
     5521 gaccttcggc cgctgccggc tcggaagctt gccgcaccgg ggcgagatct tcgaactcta
     5581 tctccatccc acctatcaag gcctcggcct tggccagaaa ttgttcgccg gcgcccgtca
     5641 caggctcggc gaactccggc taaaaggcct tctggtttgg gcccttgcag acaacgaggc
     5701 cgcttgcggc ttctatttca gcttgggtgg aaagccggtc gccgagggct cggagagctt
     5761 tggcgacacg gcgttgcgca aagtcgcctt cgcctggggc taggcgcttt accgttcgtt
     5821 aaccgcaagc gttcgacgcg ccttaacgac cctgccctat ggttgcaaac ttctactgcg
     5881 gcataggaag catcgataat gacagacgtt ggcgcacgcg accggtggat catggccggc
     5941 ctggtgatgt tggggctctt tactgtactt gccggtgtct tatcgggtcg atcgacaatc
     6001 aactacgtgc tgggccgcga tgcccgcgac gcaagcctaa cctgggccaa agaggtcaac
     6061 gatcgcttgc gcacagctga tcactccgac ctcaaggtcc tcgacaccgc cgcatttcac
     6121 agcattgtga acgatggatc cagcaccggc cttaccctgc ccacgggtgc gacgttcgca
     6181 gacgacggct tcaagcttat cgacggcttc aaccgactga cccagggctg gctccttggg
     6241 accgtcaaca ccgacagcag cgagttcgta agccagcgtg aggggtttgc cattcttgcg
     6301 ccaaccggca aggcgctggc cgccgagggc gtggagaccg aaagccaggc cactatgctc
     6361 aaggagtggg gctgcgatca agtgcagggc tactattacg gcggcccgga gacggagatc
     6421 cccgacgaca aggcggacga cacaccgctg gacatggagt cccggcccct cgaccggccc
     6481 cttgtcgcct aacgaacacg cacggcagtt atcgcgacgc gttgcgatcc tttcgcgagg
     6541 cttgtgaccg gctttccgcc gtcctagaac cacgcgggaa cagtcccgag caccctccgt
     6601 ggtcgccggg gcaacaatag cgacaacaag aaacatggcc gggcagcatg agtgacatga
     6661 ggatcgccgt tttgggcgcc gccgggcgca tgggtcaggc gttgacgcgc gcgctggccg
     6721 agacaccggg ctgcacggtg gctggcggtg tcgaggtcaa gggctcggcc gccatcggtc
     6781 aggatatcgg tgcggtcgcc gggctcgagc cgcttgggca cgcgatcacc gacgatccgc
     6841 tgccgctctt cgcccatatc gacggcgtgc tcgacttcac gctgcccaag cccacgatcg
     6901 agtatgccgc gctggcggca caagcgcgca tcgtccacgt catcggcacc actggatttt
     6961 ccgacggcga cctcgccaag ctcgaggccg ccgcgcgcca cgccactatc gtcaaggccg
     7021 gcaatatgag catgggggtg aacctgatgg ccgccctcac ggagcaggtc gcccgtacgc
     7081 tcggcccgga attcgatatc gagatcctgg agatgcatca ccgccacaag cgcgatgcgc
     7141 cctccggcac ctcgctcatg ctgggcgatg ccgcggccaa gggacgcaat gtggccctcg
     7201 gtgacaaaag cgtccgcacc cgcgagggcg acaccggacc gcgccgcgag ggcgatatcg
     7261 gctttgccgc actacgcggc ggcgacgtcg tgggcgagca caaggtgatc ttcgccggcc
     7321 ccggcgagcg catcgagatc gcacacatcg cgtcggaccg cggcattttc gcccggggcg
     7381 ccgtcaaggc cgcgctttgg ggccgaggca aaccgcctgg cctctattcc atggccgacg
     7441 tgctcggatt gtgaggactc gttgatattt tcggcatgac cgttggcaca ccatgatcaa
     7501 gcgcctaaaa actcggtgag tgccggaaag tggcctaaaa gaaaggcgat ggtggcgacc
     7561 gctttaactt tcctcgccta tagtggccgc ggccagaggc tcgcaccgag cttcgaggac
     7621 gctgaaacgc cgtaacgaga ttgccttcct agggatatgc tccaaaaggc ctgcgaagta
     7681 ccccgaaagt ccgagaacgt tctcgtgctg gtccgtcatg gtcagagcga gtggaaccgt
     7741 ctcaacatgt tcaccgggtg gaaggacgtc ggcctcagcg aggagggcgt ctccgaagcc
     7801 catcgtgccg gccagcggct aaaggaagag ggtctgcatt tcgacagcgc cttcgcctca
     7861 acgttgcggc gggcccacca taccctcgac attatcctcg gcgagctcgg ccaaggaaag
     7921 ctccccaccg tcaaagccgc cgccctcaac gagcgcgatt acggccagct cgtcggtatc
     7981 gacaaggaag aggcccgcaa gcggttcggc gccgaacagg tgcagatctg gcagcgctcc
     8041 tacgacatcg cgccgccggg tggagaatcg ctgaaggaca ccgccgcccg cgtctgtccg
     8101 tttttcgaac ggtggatcgt gccggagctg caagacggca agaacgttat cgtcgtcgca
     8161 cacggaaact cgctgcgctc gctgatcatg gagcttgacc atttgacacc cgaagaggtc
     8221 aaggactgct ccctcgccac ggcgacgccc gtgatctacc gcctcaccgt cgacggcagc
     8281 gtgaagaaga cggacctcgc cgcctaggcc atcccgctcc gcgtctcccg caacgccgcg
     8341 ttcagcgccg cggcgaaatc ttcccgctcc ggatcagtca agaagttcgc gaagaccagc
     8401 cgcttgccgt ggctggccag cgatagctcc gagttgcgcc ccacgcgctc gtcgagacga
     8461 agccgcaccc aataggggtt gaagcgccaa gactgttccc gccccttggg atcgacgcgg
     8521 gtgacaatca gggcatcgcc gcccaattcc accgtctcgt agacacgcaa agcccgaaag
     8581 ttcagcttga aggcgatgta gacgagaagc acgtcgagcc cgaagaaccc cagcaccggc
     8641 catgcgccca tgaggaagaa cagcagtccc gtgccgaacg agaccacgca cagcgccgcc
     8701 atgaagataa cgaacccctt tgggccgagc gaacggtgtg gtgtcagcac ggcgctaaac
     8761 tgggggtttt gcgcctcggg ggcttgtgat ggggtcttgg cctctgccat agcaaggagg
     8821 ctaatacaca accgcgcagt gacaaagggg cggcgataac agcctgtggc gacgcaaccg
     8881 aaagcgaaga agacggcgac accagcgctg gacggcaagc cgaaaggctc aaagctcacg
     8941 cgacgacaga tcgaggacat gttcgcgcgg cttgccgcgc gcgatccgga gcccaagacc
     9001 gagctccgct acaccgatcc ctacaccttg ctcgtcgccg tggtgctctc tgcccaagcg
     9061 acagacgcct cggtcaacaa ggcaacgccc gccctgttcg ccaccgcgga cacccctgag
     9121 aaaatggtgg cgctcggcga aaaggccgtg accgagaaga tcaagaccat cggccttttc
     9181 cgcaacaagg cgaagaacgt ggtcgcgcta tcgaggaagc tcatcgagga gcatggcagc
     9241 gcggtcccaa aatcacgcga agcgctggag acgcttcccg gcgtcggccg gaagaccgcg
     9301 aacgtggttc tcaacgtcgc cttcggcgag ccgaccattg ccatcgacac gcacatcttc
     9361 cgtgtgtcga accgcaccgg gctggcgccc ggcaaaacgc cgctcaaagt ggaagagact
     9421 ttggagcgcg ttgtccctgc gcgctacaag cacaactgcc atcactggct gatcctgcat
     9481 ggccgctatg tctgcaaagc gcgtaagccc gagtgctggc gctgcatcat cgcggacatc
     9541 tgcgtcttca aacccaagac gcccgcgccc gcgtaaagcc cgaactacgc aggctgctcc
     9601 gcgatgcgca ggcccgactt ggccgcgatg cgtttgaata c
//
LOCUS       DBNHVU010000057         5959 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_392029, whole genome shotgun sequence.
ACCESSION   DBNHVU010000057 DBNHVU010000000
VERSION     DBNHVU010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5959)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5959)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5959
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_392029"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1137
                     /locus_tag="AC16Y7_02235"
     CDS             130..1137
                     /locus_tag="AC16Y7_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5708228.1"
                     /translation="MTNPLKIAIRGAGVAGLWQALTLARRGHGVAIVERSVEPFVDAC
                     SPYAGAMLAPRCEEESAEAVIRELGTRGIALWQATYPDLMTHGSLVVAAPRDRSLLDR
                     FARMTQEPTRLDGSALAALEPGLAPRFGSALYYEDEAHVNPNTALSFLLRAVQEAGVQ
                     VHFGVGDMPQGADIVIDCRGLAAKDALPTLRGVRGERILVGAPEIAIQRPVRLLHPRF
                     PLYLVPWGNGLFMVGATAIERDDAGPVTLRSALELLSAAYVLDPAFGEAEILALGAGV
                     RPAFPDNRPRIIPAKGYIYVNGLYRHGFLLAPVLAEMVADFLETGAADPEVFLADPAQ
                     R"
     gene            1115..1312
                     /gene="thiS"
                     /locus_tag="AC16Y7_02240"
     CDS             1115..1312
                     /gene="thiS"
                     /locus_tag="AC16Y7_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018688131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HIR5708229.1"
                     /translation="MQILLNGERVTTDASTLDELCARLGFGEAKVATAVNGSFVAAGA
                     RERTALSEADEVEIVAPRQGG"
     gene            1324..2112
                     /locus_tag="AC16Y7_02245"
     CDS             1324..2112
                     /locus_tag="AC16Y7_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HIR5708230.1"
                     /translation="MLELYGKKFTSRMLLGTAGYASPDILRQAVETSGAEIVTVSLRR
                     ESARARTGQGFWELIEKLGRLVLPNTAGCRTAKEAVATAQMARELFGTTWIKLEVIAN
                     DDTLQPELFGLVEAATELCADGFQVLPYTTEDLSAAERLVAAGCDVLMPWGSPIGSAR
                     GLLNKTALHSMRAYFPETPLIVDAGIGTPSHAAEAMEMGYDGVLLNTAIAKAGDPIKM
                     AEAFAKAIQAGRIAYEAGLIEPRDMATPSTPVAGTPFFKIDHAS"
     gene            2121..2753
                     /locus_tag="AC16Y7_02250"
     CDS             2121..2753
                     /locus_tag="AC16Y7_02250"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR5708231.1"
                     /translation="MLRLDPFYPIVPDTAWLARLLPEGVKLVQLRIKEAGEDVIRAEI
                     ERGLALCADHGCQLVVNDYWREAIEAGAGLVHLGQEDLANADLAAIRGAGLKIGISTH
                     SEEELDTALAASADYIALGPIYPTKLKKMPWAPQGLPRLAEWRERVKCPLVAIGGITA
                     ERAPAVLEAGADSVAVITDIVTHQDPERRTGNWLAVTKSWRALSTGFPHG"
     gene            2831..3826
                     /locus_tag="AC16Y7_02255"
     CDS             2831..3826
                     /locus_tag="AC16Y7_02255"
                     /inference="COORDINATES: protein motif:HMM:NF017085.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HIR5708232.1"
                     /translation="MSYRLNPAMPMSEALRSVALAELDVAHSALASAPDRHKGVHNAR
                     KCFKRLRSVLLLARPGMPEPVFRNLTARVANIGKGLAAARDAQALLDAIDKLEQNTEP
                     DASDGPMRSIRGWLHKRRHAAEQNLAQTTGSEAMRKLLEIRPAIAGLAVYPDNFTPLA
                     KGIERCYRATRTHFKSAIRSDEAEELHEWRKGVQQHWRHMQLLTPCWPSELSARAEAA
                     RALSQLLGDDHDIAMLMHLVMTPTMTFGSPEDTTAFVKRCRKRHKALRKDAKIKGERL
                     FVEKPGPFASRIHAYWTTAAVGVDEPVAVPAAQEPVQQDNVVAIGGVRSGRAGQS"
     gene            complement(3823..4761)
                     /gene="pqqB"
                     /locus_tag="AC16Y7_02260"
     CDS             complement(3823..4761)
                     /gene="pqqB"
                     /locus_tag="AC16Y7_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HIR5708233.1"
                     /translation="MLIKVIGAGAGGGLPQWNCNGQNSADARHGLPTVKPRTQASIAV
                     SADGRQWVLLNASPDLRQQINATPELQPDRDGPPRNSPIKAVVLSNGDVDAVAGLLCL
                     RESQPLTVYGSARVLDVLAGNSIFDVLNPELVKREPMALGRPFAVVGPEGPVGLTVEG
                     FDVPGKIPLYLEDEEKGTGGSFGTEEGDTIGLKVTADESGKSFFFIPGCSGLDDALCA
                     RLEGASLLFFDGTLYTNDEMIAQGLMQKTGDRIGHMNMSGPKGTLAQMAPLGIARKIF
                     IHVNNSNPALRDDSAERAAVEAAGWEVSYDGMEVKA"
     gene            complement(4885..5577)
                     /locus_tag="AC16Y7_02265"
     CDS             complement(4885..5577)
                     /locus_tag="AC16Y7_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5708234.1"
                     /translation="MTHVLLIDDHPIVLQGCKKLLEDAGAQEVDEALSASAGFRLYRK
                     RKPDVIVVDLAMQAGALSGLSFLRRLRRIDQETPVLVLTMHRDPMVVGRALELGASGY
                     VLKDAPPEEVVQAFQKIRVGQSYLSHDLASDVVFTRIKEKSHKLDRLTPRELQTLTLV
                     AEGKPYGEIADELNVSYKTVANICTQLKSKLGARTRPELMRIAIQHLPENGGLGPVIH
                     EPRLGEDPIDDF"
     gene            complement(5574..>5959)
                     /locus_tag="AC16Y7_02270"
     CDS             complement(5574..>5959)
                     /locus_tag="AC16Y7_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5708235.1"
                     /translation="LATIEELAKSYGEGADLTLYRFVQEGVTAAIRKAKAKTVYIDIV
                     EVHGRGKAARETLRATLRFDGAGFTGSKPKDLTIATMVERVRAVGGSCVFRNSKNKGT
                     TIRTDIPIRRASAAAGELELVGGSL"
BASE COUNT         1081 a         1992 c         1844 g         1042 t
ORIGIN      
        1 cattgccgtg acggaggacg gccttgaagg cggcgccgac ccgcgccgcg aaggcgtcgc
       61 attaggcgat taggggaact ggacagacgc gcccgtcaaa ggcaccccgt agaaggcagc
      121 ccatagaaca tgaccaaccc cctcaagatc gcgatacgcg gcgccggcgt cgccggactg
      181 tggcaggcgc tcaccctcgc gcggcgcggc cacggcgtcg caattgtcga gcgctccgtt
      241 gagccctttg tcgacgcgtg cagtccttac gccggcgcca tgcttgcgcc ccgctgcgaa
      301 gaagaaagtg ccgaagccgt aatccgcgaa ctcgggacgc ggggcatcgc cctgtggcag
      361 gccacctatc ccgaccttat gacacatggc agcctcgtgg tcgcggcacc gcgcgaccgt
      421 agcctgctcg accgctttgc gcgcatgacg caagagccca cacggctcga cgggagcgcc
      481 cttgccgcgc tggagcccgg gctcgcgccg cgcttcggca gcgctctcta ttatgaggac
      541 gaggcacatg tgaacccgaa caccgcgctg tccttcctgc ttcgcgctgt gcaggaggcc
      601 ggcgtccagg tgcactttgg cgtgggcgac atgcctcaag gtgccgacat cgtcatcgac
      661 tgccgcggcc ttgccgccaa ggacgccctg ccgaccttgc gtggcgtgcg cggcgaacgc
      721 attctcgtgg gcgcgccgga gatcgccatc cagcggccgg tgcggctcct gcatccgcgc
      781 tttccgctct acctcgtgcc ttggggcaac gggcttttca tggtcggcgc cacggccatc
      841 gagcgggatg atgccgggcc cgtcacgctg cgctcggcgc tagagctgct cagcgccgcc
      901 tatgtgctcg atccggcctt tggcgaggcc gaaattttgg cgcttggcgc cggcgtccgg
      961 cccgccttcc cggacaaccg ccctaggatt atcccggcga aggggtatat ctatgtcaac
     1021 ggcctctacc ggcacggctt tctgctggcg ccggtcctgg ccgagatggt cgccgatttt
     1081 cttgagaccg gcgccgccga tccggaggtg ttccttgcag atcctgctca acggtgaacg
     1141 cgtcacgacc gacgcttcta cgctcgatga gctctgcgcc aggctcggct ttggcgaggc
     1201 gaaggtcgcc acggcggtca atggcagctt cgtggcggcc ggcgcacggg agcgcacggc
     1261 gctatcggaa gcagacgagg tcgagatcgt ggcgccaaga cagggcgggt gacaggacag
     1321 cagatgctcg agctctacgg caaaaaattc acttcgcgca tgctgctggg cacggccgga
     1381 tacgcctcgc ccgacatctt gcgtcaagcg gtggaaactt cgggcgccga gatcgtcacc
     1441 gtctcgctcc ggcgcgaatc cgcccgcgcc cgcaccggcc aaggcttctg ggaactgatc
     1501 gagaagctcg gccggcttgt gctacccaac accgccggtt gccgcacggc gaaagaggcc
     1561 gtcgccactg cgcagatggc gcgcgagctg ttcggcacca cttggatcaa gctcgaagtc
     1621 atcgccaatg acgacacgct ccaaccggag ctgtttggct tggtcgaagc ggccaccgaa
     1681 ctctgcgccg acggtttcca ggtgctgccc tacaccaccg aggacttaag cgccgcggag
     1741 cggctggtcg ccgcgggctg cgacgtgctc atgccatggg ggtctcccat cggctccgcg
     1801 cgcggactac tgaacaagac cgcgctgcat tcgatgcgcg cctattttcc tgaaaccccg
     1861 ctcatcgtcg atgccggtat cggcacgccg tcccacgccg ccgaagccat ggagatgggc
     1921 tatgacggcg tgctgctcaa cacggccatc gccaaggccg gcgacccgat aaagatggcc
     1981 gaggcattcg ccaaggcgat ccaggctgga cgcatcgctt acgaagccgg gctcatcgag
     2041 ccgcgggaca tggccacgcc ctccacgccc gtggccggca cgccgttctt caaaatcgac
     2101 cacgcgtcct gaccgcccac gtgctccgtc tcgacccgtt ctacccaatc gttcccgaca
     2161 ccgcctggct cgcgcgcctt ctgcctgagg gcgtcaagct cgtgcaactg cggatcaagg
     2221 aggcgggcga agacgtcatt cgcgccgaga tcgaacgggg actcgcgctc tgcgctgatc
     2281 atggctgcca actggtcgtc aacgactatt ggcgcgaggc catcgaggct ggagcaggtc
     2341 tggtgcatct cggccaggag gacttggcga acgccgatct tgccgccatc cggggtgcag
     2401 gtctcaagat cggcatcagc acccatagcg aagaggagct cgacacggcg ctcgccgcga
     2461 gcgcggacta catcgccttg ggcccgatct accccaccaa attgaagaag atgccctggg
     2521 cgccgcaagg gctgccgcgc ctcgccgaat ggcgcgaaag ggtcaagtgc cccctggtgg
     2581 ccatcggtgg catcaccgcc gaacgggccc cggccgtgct cgaagccggc gccgacagcg
     2641 tggctgtcat aacagacatt gtgacgcatc aagatcctga acgtcggaca ggaaattggc
     2701 tcgctgtcac aaaatcatgg cgggctctgt caacaggttt tccacacggc tagattccgg
     2761 tttttcggtt gaccctggac ctgaccggcg gtcatcatcg gcttgggaac ttggaggcct
     2821 gcagggacag atgagttacc ggctcaatcc ggctatgccg atgagcgaag cgctgcgatc
     2881 tgtggcgttg gctgagctcg acgtcgccca tagcgcgctg gcaagcgcac ctgatcgcca
     2941 taagggcgtc cacaacgccc gcaagtgctt caaaaggctg cgctctgtcc tcctgctcgc
     3001 gcgcccggga atgcccgagc ccgtcttccg caatctgacg gcccgcgtcg ccaatatcgg
     3061 caagggcctg gcagccgccc gcgatgccca agctcttctc gacgccatcg acaagcttga
     3121 gcagaacacg gagccagacg ccagtgacgg cccgatgcgg tcgatacgcg gctggttgca
     3181 caagcgccgc cacgccgccg agcagaactt ggcgcagacc accggatcgg aggcaatgcg
     3241 caagctgctc gaaattcggc ccgccatcgc cgggctggcc gtctatccgg acaattttac
     3301 ccccttggcg aagggtatag agcgctgtta ccgcgccacg cgcacgcact tcaagtccgc
     3361 catccgcagc gacgaagccg aggaactgca cgagtggcgc aaaggcgtcc agcagcattg
     3421 gcgacacatg cagctcctca cgccctgctg gccgtccgag ctgtccgccc gcgccgaggc
     3481 cgcgcgcgcg ctatcgcaac tgctgggcga cgatcacgac atcgccatgt tgatgcacct
     3541 cgtcatgacg ccgaccatga cttttggcag cccggaggac acgacggctt tcgtgaagcg
     3601 ttgccgcaaa cggcacaaag cattgcgcaa ggacgcgaag atcaagggcg aaagactgtt
     3661 cgtcgaaaag cctggacctt tcgccagccg catccatgct tattggacta ccgccgcggt
     3721 cggcgtggac gaacccgtcg cggtgcccgc agcgcaagaa ccggtgcagc aggacaatgt
     3781 ggtggcgatc ggcggggtgc gctcagggcg cgcgggccag agctaggcct tcacttccat
     3841 cccatcgtag gaaacttccc atccggcggc ctcgacggcg gcgcgctcgg ccgagtcgtc
     3901 gcgcagcgcc ggattggagt tgttgacgtg gatgaagatt ttgcgggcga tcccgagcgg
     3961 cgccatctgc gcgagcgtgc ccttcggtcc ggacatattc atatgaccga tgcggtcgcc
     4021 cgtcttctgc atgagaccct gggcgatcat ctcgtcattg gtgtagaggg tcccgtcgaa
     4081 gaacagaagt gacgcgcctt caagccgcgc gcacaacgca tcgtcgagcc cggagcagcc
     4141 gggaatgaag aagaaggact tgccgctctc gtccgcggtc actttgaggc cgatcgtatc
     4201 gccctcctcc gtgccgaaac tgccgcccgt tcccttctcc tcgtcctcga gataaagcgg
     4261 gatcttgccc ggtacgtcga acccttcgac cgtcaagcca acagggccct cgggcccgac
     4321 cacggcgaac ggacgcccaa gtgccatggg ctcgcgcttg acgagctcgg ggttcagcac
     4381 atcaaaaata ctgttgccgg ccagcacgtc caggacccgg gcgctgccat agaccgtcag
     4441 cggttggctc tcgcgcaggc acagaagccc ggcgacagca tcgacgtcgc cgttgctgag
     4501 aaccacggcc ttgatgggac tattccgcgg cgggccgtcc ctgtccggct gcagctcggg
     4561 cgtcgcatta atctgctgcc tgagatcggg cgaggcgttg agcagcaccc attgccgccc
     4621 gtcggcgctg accgcaatcg aggcttgcgt gcgtggcttg accgttggca ggccgtggcg
     4681 tgcatcggcc gagttctgac cattgcaatt ccactgcggg agtccgccgc ccgctcctgc
     4741 gccgatgact ttaatcaaca tgcccgtcag atggcgtctc cggcactgca tcgtcaagat
     4801 aaaaagtccg gcccatgtca gagccgggtc agcggtaagc cggcgttaag tcagcgcacc
     4861 gtcacgctct caccttggct tccgtcagaa atcgtcgatc gggtcttccc cgagcctcgg
     4921 ttcgtggatg accggaccca aaccgccatt ctccggcaga tgctggatgg cgatccgcat
     4981 caactcgggg cgggtgcgtg cgccaagctt gctcttcagc tgcgtacaga tattggcgac
     5041 cgtcttgtag ctaacattga gctcgtcggc gatctcgcca tagggcttgc cttctgcaac
     5101 caaggtcagc gtctgaagtt cacgtggcgt caggcgatcg agcttgtggc tcttctcctt
     5161 gatgcgcgta aacaccacgt cggaggcaag gtcatggctg agataggact gacccacgcg
     5221 aatcttctga aacgcttgaa caacctcctc aggcggtgcg tcctttaaga catacccgct
     5281 tgccccaagc tccagcgcgc ggccaacgac catcgggtcg cgatgcatgg tcagcacgag
     5341 gaccggcgtc tcctgatcga tccggcgtaa gcgccgcaga aaggacaagc cactcagcgc
     5401 accggcctgc atggcaagat cgacgacgat cacatcaggc ttgcgtttgc ggtaaagccg
     5461 aaatcccgcc gaggcgctaa gcgcctcgtc gacctcctgg gccccggcgt cctccaacaa
     5521 cttcttgcag ccctggagga caatcggatg gtcgtcaatc aggagaacgt gggtcaaagg
     5581 ctccctccca cgagctcaag ttcgccggca gctgcgctcg cgcgtctaat cggaatgtcg
     5641 gtgcgaatcg tggtaccctt gtttttggag ttcctgaata cgcaggaccc ccccacagcg
     5701 cgcacgcgtt cgaccatcgt ggcgatggtc aggtccttcg gcttcgaccc ggtgaaccca
     5761 gcgccatcga accgaagcgt cgcgcgcaat gtctcgcgcg ccgccttgcc gcggccgtga
     5821 acctcaacaa tatcgatata gacggtcttc gccttggctt tgcggattgc cgccgtgaca
     5881 ccttcttgaa caaaacggta gagcgtgaga tccgctccct cgccatagga cttagcgagt
     5941 tcctcgatcg tcgccaaga
//
LOCUS       DBNHVU010000058         6045 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1952635, whole genome shotgun sequence.
ACCESSION   DBNHVU010000058 DBNHVU010000000
VERSION     DBNHVU010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6045)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1952635"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="AC16Y7_02275"
     CDS             <1..266
                     /locus_tag="AC16Y7_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708236.1"
                     /translation="PDLLAGARDNVIPYDQAGEGDWKGVVERALIAARDPAWRDGILS
                     WDESAARIEAELPHPAASSAGSPAARSLPGAAGWKAQSQSIGS"
     gene            423..1118
                     /locus_tag="AC16Y7_02280"
     CDS             423..1118
                     /locus_tag="AC16Y7_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708237.1"
                     /translation="MDEVERIISRLNGIDPARPYFDPLAAEEALRQHGRLIGFRDMSF
                     TWAMGPQQANSELAGIDFESPDASRWHMTMQALRDQAEVELQRDKAMWSAYLKAREEA
                     ETRLADALHLEAFRITLINLVSGEAGEVSNSVACLASTAMREAIAGNSVENESLQHLN
                     DVYMPFVDALLAGLGSFWIVDERFVCLPLPRLALEEGQLVSDGRPAAVWPNGEAYANG
                     DEGLIPVLQSAEW"
     gene            1174..1485
                     /locus_tag="AC16Y7_02285"
     CDS             1174..1485
                     /locus_tag="AC16Y7_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708238.1"
                     /translation="MREHHAIGLAACLLGATLISVTAPALAAKIKCQGIFQVMKHGLL
                     STPYCQDQEIARVARSYGWKVTVAQVRNDPLKKVYICQALGNDNRLKGACGAYSSRRY
                     R"
     gene            complement(1514..2257)
                     /gene="gpmA"
                     /locus_tag="AC16Y7_02290"
     CDS             complement(1514..2257)
                     /gene="gpmA"
                     /locus_tag="AC16Y7_02290"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF010713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HIR5708239.1"
                     /translation="MPRLILLRHGQSVWNRDAVFTGWTDVDLCDNGITEAKHAAALLK
                     EHNIEFDQCYASYLQRSVHTLWMVLDALELTWLPVRRSWRLNERHYGALQGRKRDEVM
                     EEVGPEQLHIWRRSYTVRPPALTEADPRFPGNQSKYDRVPRRHLPLTESLKDCVARVL
                     PFWQERVEPLLMDGRTPLISAHGNSLRGLVKYLDGISDEDIPHLEIPTGKPLVYELDE
                     GLRTVRSFYLEDGKEAHALPKAAEAAKTA"
     gene            2488..4806
                     /locus_tag="AC16Y7_02295"
     CDS             2488..4806
                     /locus_tag="AC16Y7_02295"
                     /inference="COORDINATES: protein motif:HMM:NF017021.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR5708240.1"
                     /translation="MRLIFSILTAIVVIIAALVFVGPMFISTEELRNQLFARVESMTG
                     YRLRVSGPVDITLFPSIDLVAEDVGVAQPADRGDAEFATAKTLRFSLMLRSLFDGKVR
                     VKTITLVDPVIAIPQKTAAPAAPAAPALAPMPAPGDSQASPAQGGGSSGLARVAEQLK
                     TVTLDKLVIKDGTVILPPSDGAPGKRIEKLNLNASLPDYDKPLSFAVSAVVDGKAYKS
                     EGAIGNFGGFLEGAPAPVRLKVEAPGALDAPASLGGTATYKNDTLTFSQFSAKSGDKT
                     LAGSAVYKDGVATLSQLTGTVGRDTFAGHAVYKDNVVTVRSLRANVRGAVLAGSLTVN
                     LANKVPYVVAAFAAKTLDVNALTGAPKSKPKSKPKPTPTPTAKAKPSSGGGGGGSGGG
                     SKTKANAGKSGWSSARIDFSPLKLVNGKFSLTAEQLIYDHVKLSPVSVQATLNGGKLN
                     ASLSKFNLYGGAGNAAIAVDASRKTPTQQVNLAVTKFNAYPLLKDAADFERIEGKGTV
                     NLKLSGVGASQRAIVSSLNGTANLEFVDGAVRGVNIAKMIRKVGIGVVDGWQGGKTEK
                     TDFASLGASFQIAKGQATTSDMHLVGPLVKMTGAGTVNLPAQSLKFRVEPQLVASLEG
                     QGRQGDIAGLGVPVMIVGPWSKPKIYPDIKGILQDPAGAIARFKDFGDGVKKLPGLEN
                     IDQSGVLQNVIKDGKVDQDALIQGIGGLLGGTQTAPAPPPPPAQAVPQGAAPAAAQPK
                     PPAQPKQKKKVDAEDIAKDAARQLLNDFLGGR"
     gene            4868..5353
                     /locus_tag="AC16Y7_02300"
     CDS             4868..5353
                     /locus_tag="AC16Y7_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR5708241.1"
                     /translation="MRSILHLCQDAPLQELPAGTVLLSEGETTGHLYVLKSGEIEVLR
                     GETQVAVIEDPGAVFGEMSVLLKRSHTATVRTLAPASLYVFDDAESFLRSNPEIALFI
                     GRLLAGRLNAATSYLADLKRQFEDQGNHLGMVGEVLDVLIHQQDEDFSPGSEDGADPR
                     L"
     gene            complement(5313..>6045)
                     /locus_tag="AC16Y7_02305"
     CDS             complement(5313..>6045)
                     /locus_tag="AC16Y7_02305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR5708242.1"
                     /translation="FDVVVMAGDHLDLASLVDRRAQAVVVKKYFARIQAKTRLLICSG
                     NHDLDAENEAGEQTAKWLLGARNKGVLSDGDSVLIEDTLFTMCAWWDGPVSREAIGAQ
                     LAEAAKKRPKHWIWIYHAPPANSPVAWGGQRYFGDDALKKWIEEYRPDIVMSGHVHEA
                     PYVDKGSWVDRIGQTWIFNTGHYLGAPPAHIMMDTKAGEVLWFSAAGNQSVELDSQLT
                     RPPAKLTALPEWLTAGDPPHPQSQG"
BASE COUNT         1139 a         1823 c         1936 g         1147 t
ORIGIN      
        1 cccctgatct cctggctggc gctcgggaca atgtcattcc ttacgaccaa gcgggtgagg
       61 gcgactggaa gggcgtcgtc gaacgtgcgc tcatcgcggc tcgagatccg gcgtggcgcg
      121 acggcatcct gtcgtgggac gagtccgccg cgcggatcga agcggaactt ccgcacccgg
      181 cggcgagctc tgccggttct cctgcggcgc gatccctccc gggcgccgcg gggtggaagg
      241 ctcagagcca gtccatcggc tcctgagtca cttcatgaca tgaaatggtg ggctgcgcgg
      301 tgtaggatcg ccgaccagac aactggaccg gcagccaatg ccggcacgct gcgagccaca
      361 aatggctggg ttttgcgttc acacgcgtcc gagggaggac gcttctgggg gagggcaacg
      421 gcgtggacga agtagagagg atcatctcgc gacttaacgg gatcgacccc gcgcgtccct
      481 attttgatcc gcttgccgcg gaagaggcgc tgcggcagca tggccggctc atcggctttc
      541 gggacatgtc ctttacctgg gccatgggac cgcagcaggc caatagcgaa ttggcgggca
      601 tcgattttga aagtcccgat gccagccgct ggcatatgac gatgcaggcg ttgcgcgatc
      661 aggcggaggt cgagcttcag cgcgacaagg cgatgtggtc ggcctatctg aaggcgcgcg
      721 aagaagccga gacgcggctc gccgacgcac tccatctgga ggcgttccgc atcacactga
      781 tcaatctcgt gtctggcgag gccggtgagg taagcaacag cgtggcatgt cttgcatcga
      841 ccgcgatgcg cgaagcgatc gccggaaaca gcgtcgagaa cgagagcctc cagcacttga
      901 acgacgtcta tatgcccttc gtggacgccc tgttggcagg gctcggctcg ttttggattg
      961 tcgacgaacg gttcgtttgc ctgccgttgc cgcggcttgc gctggaggag gggcaattgg
     1021 tgagcgacgg gcgtcccgcc gctgtttggc ccaacggtga ggcctacgcc aatggagacg
     1081 aagggctcat tcctgttcta caatccgcag agtggtgagt tgcggcgcca catggcgcgc
     1141 aattcctttt gcggctttcg atgggaggca gacatgcgcg aacatcatgc aatcggtttg
     1201 gcggcgtgcc ttcttggcgc gacgctcata agcgtgacgg cgcctgcgct cgcggccaag
     1261 atcaaatgcc agggcatctt tcaagtcatg aaacacggtc ttctctcgac gccttactgt
     1321 caggatcaag agatcgcgcg tgtggcgcgg agctatggct ggaaggtgac cgtggcgcag
     1381 gtccgcaacg acccgttgaa gaaggtctac atttgtcagg ccctcggcaa cgacaaccgg
     1441 ctcaagggcg cgtgtggcgc ttactcgtcc aggcggtacc ggtaggctgc ggcgaacatc
     1501 aaccaatctg cttctaagcg gtcttggctg cctcggccgc tttgggcagg gcgtgggctt
     1561 ccttcccgtc ctcaagatag aaactgcgga cggtacgcag gccttcgtca agttcgtaaa
     1621 ccagcggctt tcccgttggg atttccaggt gcgggatatc ctcgtcggaa atcccgtcga
     1681 gatatttgac gagacctctg agactatttc cgtgcgccga gatcagcggc gtgcgtccat
     1741 ccatgagaag gggttcgacg cgctcctgcc agaagggcag cacccgggct acgcagtcct
     1801 ttaggctttc ggtcagcggc aggtggcggc gcggcacccg gtcatatttc gactgattac
     1861 cgggaaagcg cggatcggcc tcggtgagtg ccggcggccg gacagtgtaa ctgcggcgcc
     1921 aaatgtgcaa ctgctccggc ccgacttcct ccatgacctc gtcgcgcttg cgtccctgca
     1981 gcgcgccgta gtggcgctca ttgagccgcc agctgcgccg cacgggaagc caggtgagct
     2041 ccagcgcatc cagcaccatc cagagcgtat gaaccgagcg ctgcagatag gaggcgtagc
     2101 actgatcgaa ctcgatgttg tgctctttca gcaatgccgc ggcatgcttc gcctcggtga
     2161 tgccgttgtc gcagaggtcc acatcggtcc agccggtgaa gaccgcatcg cggttccaga
     2221 cgctttgacc gtgtcgcagg agaataagtc gcggcatacg aagtctccgt tgtaggtgac
     2281 ggcaacaggt gtatgggtcc gagccttccg gggcaagcct ccaggggcgc gcgccggccg
     2341 gttattagcc gtcatttgcc ctcaatccgg gtctattcca atgcatcagg cccaagtcag
     2401 gcgtagcctt ttcaggggat tatcccttag tttggaattt gtccgtatct cggtaaattc
     2461 caccagcaat cgctttcgga ggggctcgtg cggctcatct tctcgatcct gaccgccatc
     2521 gtggtcatta tcgcggcgct cgtttttgtc ggcccgatgt tcatctccac cgaggagctg
     2581 cgtaaccagc ttttcgcgcg ggttgagtcg atgacgggat acaggctccg ggtcagcggt
     2641 ccggtggata tcaccctgtt tccgtcaatc gatctggtgg ccgaagatgt cggggtcgct
     2701 cagcccgcgg atcgcggcga tgccgagttc gccaccgcca agacccttcg gttcagcttg
     2761 atgctgcgca gcttgttcga cggcaaggtg cgggtgaaga cgatcacact cgttgatccg
     2821 gtgattgcca tcccgcagaa aactgctgcc ccggccgctc cagccgctcc ggcgctagcg
     2881 ccaatgccag cgcccgggga tagccaggca agcccggctc aaggcggcgg tagcagcggg
     2941 ctcgcacgcg tggccgaaca gttgaagacc gtcacgctcg acaagctcgt gatcaaggac
     3001 ggaacggtga tcctgccgcc ttcggacggc gcgccgggca agcggatcga gaagctcaat
     3061 ctcaacgcgt cgctgccgga ctacgacaaa ccgttgtctt ttgcagtgag tgccgttgtc
     3121 gatggcaagg cgtacaagag cgaaggggcg atcggcaatt tcggtggctt tctggaaggc
     3181 gcgcccgcgc ctgtgcggct caaggtcgag gcgccggggg cgctcgatgc gcccgccagt
     3241 ctgggcggta cggcgacata caagaacgac acgctcacgt tcagccagtt tagcgccaag
     3301 tccggcgaca agacgctcgc aggatccgcg gtctataaag atggcgtcgc aacgttgagc
     3361 caactcaccg ggacggtggg acgggatacc ttcgccggtc atgccgtcta caaggacaac
     3421 gtcgtcaccg ttagatcttt gcgcgccaat gtgcgcggcg ccgttctggc cggctcgctg
     3481 acggtgaatc ttgccaacaa ggttccttac gtcgtagcgg cgtttgcggc gaagacgctg
     3541 gacgtcaacg cgctcacggg cgcgccgaaa tcgaagccga aatcgaagcc gaagccgacg
     3601 ccgacgccga cggctaaggc aaagccgtcc tccggcggag gcggtggcgg tagcggcggc
     3661 ggttcaaaaa cgaaagccaa tgcgggcaag tcgggctgga gcagtgcgcg aatcgacttc
     3721 tcgccgctca agctcgtcaa tggcaagttc agcctcaccg ccgagcagct catctacgat
     3781 catgtcaagc tcagcccggt gtcggtgcaa gcgacactca acggcggaaa gctcaacgct
     3841 tcgctgtcga agttcaatct ctatggcggc gcaggcaatg ccgcgattgc cgtcgatgcg
     3901 agccgcaaga cgccgacgca acaggtcaat ctcgccgtca ccaagttcaa tgcctatcct
     3961 ttgctcaagg acgccgcgga ctttgagcgc atcgagggca agggcacggt caacctcaaa
     4021 ctgagcggcg ttggcgccag ccagcgcgcc attgtctcga gcctcaacgg cacggccaac
     4081 ttggagttcg tcgatggtgc tgttcgcggc gtcaacattg ccaagatgat ccgcaaagtc
     4141 ggcataggcg tcgtcgatgg atggcagggc ggcaagaccg agaagactga ctttgcctcg
     4201 cttggtgcca gcttccagat cgccaagggg caggccacca ccagcgacat gcacctggtc
     4261 ggtccactgg taaagatgac cggcgccgga acggtcaatc tgcccgcgca aagcctaaaa
     4321 ttcagggtcg agccgcagct cgtggcatcg ctcgaaggtc aggggcggca gggggacatc
     4381 gccggtctcg gcgtgccggt catgattgtc gggccttggt ccaagccgaa gatttatccc
     4441 gacatcaagg gcatcctgca ggacccggcc ggcgctattg cgcggttcaa ggacttcggc
     4501 gacggtgtga agaaactgcc gggcctggag aacatcgatc agagcggcgt gctgcagaat
     4561 gtgatcaagg acgggaaggt cgatcaggac gcgttgatcc agggcattgg cggtctgctc
     4621 ggcggcacac aaaccgcgcc tgcgccgccc ccaccgccgg cgcaagccgt accccagggc
     4681 gccgcgccgg cagcggctca gccaaagccg cctgctcagc cgaagcagaa gaagaaggtc
     4741 gatgccgaag acatcgccaa ggatgccgcc aggcagttgc tgaacgattt tctcggcggg
     4801 cgatagcgcc gacccacgcg gcaccaggtc tcgttgacaa cgctagaggt gaggatacgc
     4861 tcacgtcatg cgttcgatcc tccacctgtg ccaagacgca ccgcttcagg agctgccggc
     4921 cggcacggtt ctgctgtcag agggcgagac gacaggccat ctctacgtgc tcaagtccgg
     4981 tgagatcgag gtgttgcgcg gcgaaacgca agtcgccgtg atcgaggatc ccggagccgt
     5041 cttcggcgag atgtcggttt tgctcaagcg gtcgcatacg gccacggtac ggacgctcgc
     5101 gcccgcctct ctctacgttt tcgacgatgc ggagagtttt ctcaggtcca acccggagat
     5161 tgcgttgttt attggccgct tgctcgccgg gcgtttgaac gcggcgacca gttatctggc
     5221 cgacctcaag cgccagttcg aagaccaagg caaccatctc ggcatggttg gcgaggtgct
     5281 cgatgtcctg atccatcagc aggacgaaga cttcagccct ggctctgagg atggggcgga
     5341 tccccggctg tgagccactc gggcagcgcg gtgagcttcg ctggtggccg cgtcagttgg
     5401 ctgtcgagtt cgacggactg attcccggcg gctgagaacc acaacacttc gcctgctttc
     5461 gtatccatca tgatatgtgc tggcggcgcg ccgaggtagt gcccggtgtt gaatatccag
     5521 gtctggccga tacggtcgac ccaagagcct ttgtcgacat acggcgcttc gtgcacatgt
     5581 ccggacatca cgatgtcggg ccggtactcc tcaatccatt tcttgagtgc gtcatcgcca
     5641 aaatagcgtt ggccgcccca agcgacgggc gagttcgccg gcggtgcgtg atagatccag
     5701 atccagtgtt tcggacgctt cttagcggcc tcggcaagct gcgctccaat tgcttctcgc
     5761 gacaccggtc cgtcccacca agcgcacatc gtgaacagcg tgtcctcgat gagaacagag
     5821 tcgccatcgg acagcacgcc tttgttgcgc gcgccgagaa gccatttggc cgtctgctcg
     5881 cctgcctcgt tctcagcgtc gagatcgtga ttgcctgaac agatcagaag ccgtgtcttc
     5941 gcctgaatgc gcgcaaagta cttcttcacg acgacggcct gggcgcggcg atcgacgagc
     6001 gaggcgagat cgagatggtc tcccgccatc accacaacgt cgaag
//
LOCUS       DBNHVU010000059         6744 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_244912, whole genome shotgun sequence.
ACCESSION   DBNHVU010000059 DBNHVU010000000
VERSION     DBNHVU010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6744)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_244912"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /gene="msrA"
                     /locus_tag="AC16Y7_02310"
     CDS             complement(<1..507)
                     /gene="msrA"
                     /locus_tag="AC16Y7_02310"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR5708243.1"
                     /translation="MIARWICVGLTAAFLAMFMVASGADDTSAGESKTAIFAGGCFWC
                     VEDAFDNVDGVSETVSGYAGGTLPEPTYGRHEGHVEALKVTYDPSKVTYAQLLDHYWH
                     NIDPFDATGQFCDKGPAYRTVVFTSDEEERTLAEKTKKEISTRFNKTVATEIRPTTTF
                     TAAEDYHQD"
     gene            660..2315
                     /locus_tag="AC16Y7_02315"
     CDS             660..2315
                     /locus_tag="AC16Y7_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5708244.1"
                     /translation="MQTTLSLPQDAMKPAYDVVVVGSGYGGGVSASRLSRAGQRVCVI
                     ERGREVPAGGFPARLPELRRELQLSGGTMRSGSKTGLYDFRIGADIHVLVGCGLGGGS
                     LVNAGVALRPDARVFADAAWPDAINKDGLLDLGFERAEAMLRPARYGNAAALTKYRAL
                     ETAGAAFGRATVASPVTVSFEDTENAAGVTQAACTLCGDCCSGCNVGAKNTVAATYLP
                     DARAHGAEIFTELTVDYIEKRDGGWRVHFAPSGDKNGEARHVDARSVVLAAGTLGSTE
                     ILLRSRDQGLKCSDRLGTRFSANGDIIAFALGGSERVNAIGVGEPPKFDGDTVGACVA
                     GQIELPDEKDLDRGMIIEEGVLPSALAPLLPVFFISGGRILGAAQSLIKGVYQGPLSH
                     LRTFFVVSHDDADGRLVLDDGRLKVRWPNVAEQPVYKRVDEALTKVADTVGGRYIKSP
                     LAATSMGVTPATAHPLGGCGMGEDARRGVVNHKCQVFADTNGEDVHDGLYVCDGAVIP
                     RSIGCNPLLTITALTERAMVHLARDHNLGFDTEPIREAEQAAS"
     gene            2312..3724
                     /locus_tag="AC16Y7_02320"
     CDS             2312..3724
                     /locus_tag="AC16Y7_02320"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR5708245.1"
                     /translation="MRIGCSILLGAAALALSLVASASASQGAVRITPCASTEYTASIG
                     DAPAQTPTQSPDQLTIVLVGDTGFNPKGAEVDPRGFKKNGKEIGFAESVSGVASDING
                     DLAFVNLETVITDRDDLEPDGKGQTSPYNFKSHPAGLRALLNTGFNLFSLANNHSMDY
                     GPLGVEETLYHLAVAGAEHPIAYAGIGTNYEEATRPGCLELGDMKLGFSATGIVTGQR
                     EEHRAGQNKAGQAAYRNPKDFQLVLNRLAELPADYRILSIHYGLEGRVVPDKRQLTDW
                     RGKAAQGRGIDLIVGHHPHVAQGVERVGNSLIFYGLGNFLHPGTAEMKRFGVCRDYGL
                     MAKVHLAKQDGDWRLGTIEAVPLTGTHMKPERFSPQQSATRIHALNHLGARLGDGGTA
                     QGVLFTPRSDGSGLYCSDDANALGGKIGAICKGYQPAPATPASIARQLASACADKPFY
                     GAGKKKRKRWQRRRSGFERR"
     gene            complement(3721..6132)
                     /gene="pheT"
                     /locus_tag="AC16Y7_02325"
     CDS             complement(3721..6132)
                     /gene="pheT"
                     /locus_tag="AC16Y7_02325"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR5708246.1"
                     /translation="MKFTLSWLKEHLDTDASVDEIADTLTRIGLEVEEVHNPAAALKE
                     FRIAEVVKANKHPDADKLQVLEVNTGKERLQVVCGAPNAHAGMKGVFAPVGAYVPGLD
                     FTLTKAKIRGVESSGMMVSEKELELSDEHTGIIELPKKARVGSSAAKALGLDDVMFDI
                     AITPNRPDCLGVRGIARDLAAAGLGKLTKETIKPVKGGFKNSVPIKLEFSTETKTACP
                     VFAGRLVRGVENGPSPDWLQARLRAIGLRPINALVDITNYISYDRGRPLHVYDADKLD
                     GAIRARLGRKGEKFLALDGKTYAVDEEMCVIADDARVLGLGGVIGGEDTGCTEETTNV
                     FIESAYFDPKRTARTGRTANIVSDARFRFERGVDPQSVLPGIEFATELVLELCGGEPS
                     QVTVAGKAPKSNSPFRFDMRQIGRLSGLSLSNADIRRKLTALGIKLGGKTNAVKASPP
                     SWRPDIAIPADLVEEVVRLTGVDQVPATPLPDLSGVAKPVLTERQARAKFTRRLLAAR
                     GFVEAVTWSFNPPAEAKLFGGGDKSLTLSNPISTELAQMRPSLLPGLIAAAKRNRDRG
                     FADGALFELGQAYRGLAPEDQYMAASGVRFGRSALDGAGRHWSGDADPAGWEAVKADA
                     VAALTALGLSQANMQVTREAPDWFHPGRSGALKLGPKVTLGVFGEIHPDTLASLDAEA
                     PMAAFELYLDALPRSKRKGTARPALDASDLQAVRRDFAFLVDADVAAGQVVRAASGAD
                     RTLIADVSVFDVFTGQGVPDGKKSLAIDVTLQPRDKTLTDAEIDAVAEKVVAAVTKAT
                     GGALR"
     gene            complement(6129..>6744)
                     /locus_tag="AC16Y7_02330"
     CDS             complement(6129..>6744)
                     /locus_tag="AC16Y7_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA ligase subunit PheS family protein"
                     /protein_id="HIR5708247.1"
                     /translation="DTFYFAEGPDGARPVLRTHTSPVQIRTMLANEPPLRVIAPGRVY
                     RCDSDQTHTPMFHQVEGLVIDEETHLGHLKWVLEEFCKAFFEVPEVKMRFRASYFPFT
                     EPSMEVDIGYAKVGDEIRIGEGDEWLEILGCGMVHPNVLKAVNLDPKKHQGFAFGVGI
                     DRLAMLKYGMPDLRAFFSGDLRWLTHYGFASLAVPTLAAGLSAR"
BASE COUNT         1185 a         2171 c         2165 g         1223 t
ORIGIN      
        1 gtcctggtga tagtcctcgg cggcggtgaa ggtcgtcgtg ggacgaatct ccgtggcgac
       61 ggtcttgttg aagcgcgttg agatttcttt cttggtcttt tcggccagcg ttctttcttc
      121 ctcgtccgag gtgaacacca ccgtgcggta ggccgggcct ttgtcgcaga actgacccgt
      181 cgcatcgaat gggtcgatgt tgtgccaata gtggtcgagc agttgcgcgt aggtgacctt
      241 cgatgggtcg taggtgacct tcaacgcctc cacatggccc tcgtgtctgc cataggtcgg
      301 ctcgggcagc gtgccgccgg catagccgga gacggtctcg ctcacaccgt cgacattgtc
      361 gaaggcgtcc tcgacgcacc agaagcagcc gccggcgaag atcgcagtct tgctctcgcc
      421 cgcagacgtg tcatcggcgc cgctggcgac catgaacatg gcgagaaagg ccgccgtcag
      481 cccgacgcaa atccaacgcg caatcatgag atgtctccac attgttgtct ggctctaacg
      541 ataggcaacg ggcgcgagcg cgcctaggca ctctcgcggc accgtgatgg gaagtgatgc
      601 gtcatttggg gccgcataac cgcttggggc gacttgctga tttgggttat tgaggggcca
      661 tgcaaacgac gttgtcttta ccccaggacg ccatgaaacc ggcctatgac gtggtggtgg
      721 tgggctccgg ctatggcggc ggggtctccg cctccaggct gtcccgggcc gggcaacgcg
      781 tctgcgtcat cgagcggggc cgggaggtgc cggcgggagg ctttccggcg cggctgcccg
      841 aactgcggcg cgagctgcaa ctgagcggcg gcaccatgcg ctccggctcc aagaccggcc
      901 tctacgactt ccggatcggc gccgatatcc acgtgctcgt gggctgtggg ctcggtggcg
      961 gctcgctggt caatgccggc gtcgccttgc gccccgatgc gcgcgtcttt gccgatgccg
     1021 cctggccgga cgcgatcaac aaagacggcc tgctcgatct cggctttgaa cgtgcggagg
     1081 ccatgctgcg gccggcgcgg tacggtaacg ctgccgcctt gaccaagtac cgtgcgctgg
     1141 aaaccgccgg cgccgcgttc ggccgcgcca cggttgcctc gcccgtcacg gtgagcttcg
     1201 aggatactga gaatgcggcc ggcgtaactc aagccgcgtg cacgctgtgc ggcgattgct
     1261 gttcgggctg caatgtcggc gccaagaaca ccgtggccgc gacctatctc cccgatgcac
     1321 gcgcgcacgg cgccgagatc ttcaccgagc taactgtcga ctacatcgag aagcgcgacg
     1381 gcggctggcg cgtgcatttc gcaccctccg gcgacaagaa cggcgaagcg cgccatgtcg
     1441 atgcacgctc ggtggtgctc gccgccggca cgcttgggtc cacggaaatt ttgttgcggt
     1501 cgcgcgatca aggattgaaa tgctccgacc ggctgggcac gcgcttttct gccaacggcg
     1561 acatcatcgc ctttgcgctc ggcggaagcg aacgggtcaa cgccatcggt gtcggcgaac
     1621 cgccaaagtt tgacggtgac acggtcggcg cttgcgttgc cgggcagatc gagcttcccg
     1681 acgagaagga cctcgatcgt ggcatgatca tcgaagaagg cgtgctgccc tctgccttgg
     1741 cgccgctgct gccggtgttc tttatatcgg gggggcgaat tctcggcgcc gcacagagcc
     1801 tcatcaaggg cgtgtatcaa ggcccgctgt cgcacttgcg cacgttcttc gtcgtctctc
     1861 atgacgacgc cgacggccgg ctcgtgctcg acgatggacg cctcaaggtt cgctggccga
     1921 atgtcgccga gcaacccgtc tacaaacgcg ttgatgaggc cctgaccaag gtggccgata
     1981 cggtcggcgg gcgctacatc aagagcccgc tcgccgcgac cagcatgggc gtgacacccg
     2041 cgacggcgca tccgcttggc ggctgcggca tgggtgagga cgcgaggcgc ggcgtggtca
     2101 accacaaatg ccaggtcttc gccgatacca acggcgagga cgtccatgac ggtctctatg
     2161 tctgcgacgg cgccgttatt ccgcgctcca tcggttgtaa cccgctgctg accatcaccg
     2221 ccctgaccga gcgcgccatg gtccatcttg cccgcgacca caatctggga ttcgatacag
     2281 aaccgattcg cgaggcggaa caggccgcat catgaggatt ggctgttcga tattgctggg
     2341 cgccgccgcc ttggccctct cgcttgtcgc gagcgcgagc gcttcgcaag gcgccgtgcg
     2401 gatcacgcca tgcgccagca ccgaatacac cgcctcgatc ggcgatgccc cggctcagac
     2461 gccgacacaa agccccgacc aactcaccat cgtgcttgtc ggcgataccg gcttcaatcc
     2521 gaaaggcgca gaggtcgacc cgcgcggctt caagaagaac ggcaaggaga tcggctttgc
     2581 ggaaagcgtg agcggcgttg cgagcgacat caatggcgac cttgccttcg tcaatctaga
     2641 gacggtgatc actgaccgcg acgatcttga gcccgacggt aaaggccaga cctcgcctta
     2701 caacttcaag agccacccgg cgggcttgcg tgcgttgctc aacaccggct tcaatctgtt
     2761 ctctctcgcc aacaatcatt cgatggacta cggcccgctc ggcgtcgaag agacgctcta
     2821 tcacttggct gtggcggggg cggagcatcc gattgcttat gccggcatcg gcaccaatta
     2881 cgaggaggcg acgcggcccg gctgtctcga gctcggcgac atgaagctgg gcttcagcgc
     2941 taccggcatc gtcaccggcc agcgggagga gcatcgcgcg gggcaaaaca aggcggggca
     3001 ggcggcctat cgcaatccaa aggatttcca gctggtgctg aaccggctcg ccgagcttcc
     3061 ggccgactac cgcatcctgt cgatccacta tggcctcgaa ggccgtgtcg tgccggacaa
     3121 gcggcagttg acggattggc ggggcaaggc ggcgcaaggc aggggcatcg acctcattgt
     3181 cggccatcac ccgcacgtgg cccaaggcgt cgagcgcgtc ggcaactctc tcatcttcta
     3241 tgggctcggc aatttccttc atcccggcac cgcggagatg aagcgcttcg gcgtttgccg
     3301 cgactatggg ctgatggcga aagtgcatct ggccaagcag gacggcgatt ggcgtctcgg
     3361 caccatcgaa gcggtcccgc taacgggcac gcatatgaag cccgaacgct tctctccgca
     3421 gcaaagtgcg acgcgcatcc atgcgctgaa ccatctcggc gcgcggctcg gcgacggcgg
     3481 cacggcacaa ggcgtgctct tcacaccgcg tagcgacggc tcggggcttt attgctcgga
     3541 cgatgcaaat gcgctcggcg gcaagatcgg cgccatctgc aaaggctatc agccggcgcc
     3601 cgcgacgccg gcgagtatcg cgcggcaact tgccagcgct tgcgccgaca agccgttcta
     3661 tggcgctggg aagaaaaagc ggaagcgctg gcagcggcgc agatccggtt tcgagcggcg
     3721 ctagcgcaac gccccgccgg tggctttggt cacggcggcg acgacctttt cggccaccgc
     3781 gtcgatctcc gcgtccgtga gcgtcttgtc gcgcggctgc aacgtcacat cgatggcgag
     3841 tgactttttg ccgtccggca caccctgacc ggtgaacacg tcgaacacgc tgacatcggc
     3901 gatcagcgtg cggtcggcac cggaggctgc gcgcacgacc tggcctgcgg ccacgtcagc
     3961 atccacaaga aacgcaaagt cgcgccgcac cgcctggagg tccgaggcat cgagcgcagg
     4021 cctcgccgtg cctttgcgct ttgaacgggg caaggcatcg agataaagct cgaacgccgc
     4081 catcggcgct tcggcgtcga ggctggcgag agtgtcggga tggatctcgc cgaacacgcc
     4141 gagcgtgacc ttcggcccga gcttcagtgc acctgaacgt cccggatgga accagtccgg
     4201 cgcctcgcgg gtcacttgca tgtttgcctg actgaggccc agcgcggtga gcgcggcgac
     4261 ggcgtcggcc ttcaccgcct cccagccggc ggggtctgca tcgcctgacc agtgacggcc
     4321 agcgccatcg agcgccgaac gcccgaagcg cacgccgctc gcggccatgt attggtcttc
     4381 aggggcgagg ccccgatagg cctggcccag ctcgaacagt gcgccgtctg cgaaaccgcg
     4441 atcgcgattg cgcttggctg ccgcgatcaa gcccggcagc aggctgggcc gcatttgagc
     4501 aagttccgtg gagatcggat tactcagcgt gagcgacttg tcgccaccgc cgaacagttt
     4561 cgcctcggcc ggcggattga aagaccaggt gacggcctcg acaaatccac gtgcggcaag
     4621 gaggcgccgc gtgaacttcg cgcgcgcttg gcgctcggtc aggactggct tggccacacc
     4681 agacagatcg gggagcggcg tagccggcac ctgatcgaca ccggtcagcc gcaccacctc
     4741 ttcgacgaga tcggctggta tggcgatgtc cgggcgccag gagggcgggc tcgccttcac
     4801 agcgttcgtc ttgccgccca gcttgatgcc gagtgcggtg agcttgcggc ggatgtccgc
     4861 gttcgacagt gacagcccgc tcaagcggcc gatctgcctc atgtcgaagc gaaagggcga
     4921 attggatttt ggcgccttgc cggcaaccgt gacctggctc ggttcaccgc cgcacagctc
     4981 gagcaccagt tcagtggcga attcgatccc cggcagcacg gattgaggat ccacgccgcg
     5041 ctcgaagcgg aagcgcgcgt ccgaaacaat gttcgccgtg cggccggtgc gcgcggtgcg
     5101 cttgggatcg aaataggcag actcgatgaa gacattcgtc gtctcctcgg tgcagcccgt
     5161 gtcctcgccg ccaatcacgc cgccaagtcc aagcacgcgc gcatcgtccg cgatgacgca
     5221 catttcttcg tcgactgcat aggtcttgcc gtcgagggca aggaacttct cgcccttgcg
     5281 tccaagccgc gcgcgtatgg cgccgtcgag cttgtccgcg tcgtaaacat gtaagggacg
     5341 cccacggtcg taagagatgt aattggtgat atcgaccagc gcattgatgg ggcgcaaacc
     5401 gatagccctc agccgcgctt gcagccagtc cgggctcggc ccgttctcaa cgccgcgcac
     5461 aagccgcccg gcaaacacag ggcaagccgt cttggtctcc gtcgagaact ccagcttgat
     5521 aggaacggag ttcttgaacc cgcctttgac cggcttgatc gtttccttcg taagcttgcc
     5581 gaggcccgcc gcggcgagat cgcgcgcgat cccgcgcacg ccgagacaat cggggcggtt
     5641 cggcgtgatg gcgatgtcga acatcacatc gtcgaggccg agcgcctttg cggccgaact
     5701 gccgacgcgg gccttcttcg gcaactcgat gatcccggta tgctcgtcgg acagttcaag
     5761 ctccttctcg gagaccatca tgccgctcga ctcgacgccg cggatcttcg ccttggtcaa
     5821 cgtaaagtcg aggccgggca cgtaagcgcc gacaggcgcg aacacgccct tcatcccggc
     5881 atgggcgttc ggcgcgccac acacgacttg caggcgctcc ttgccggtgt tgacctcaag
     5941 cacttgcagc ttgtcggcgt ccgggtgctt gttcgccttc accacttcgg cgatgcgaaa
     6001 ttctttgagc gccgcggccg ggttgtgcac ctcttcgact tccaacccga tgcgggtcag
     6061 cgtgtcggcg atctcgtcca cggaggcgtc cgtgtcgaga tgttccttca gccatgacag
     6121 ggtgaacttc atcgcgcgct cagccccgca gcaagtgtgg gaacggcgag cgacgcgaaa
     6181 ccgtaatggg tcagccagcg caaatcgcct gagaagaacg cgcgcaagtc aggcatgcca
     6241 tatttcagca tggccaagcg gtcgatgccg acgccaaacg caaatccctg atgtttcttg
     6301 gggtcgaggt tcaccgcttt cagcacgttg ggatgcacca tgccgcagcc gagaatctcg
     6361 agccactcgt cgccttcgcc gatgcggatc tcatcgccca cttttgcata gccaatgtcg
     6421 acttccatcg acggctcggt gaaggggaag tagctggcgc ggaagcgcat cttgacttcc
     6481 ggcacttcga agaaggcttt gcagaattct tcgagcaccc atttcaggtg accgagatgc
     6541 gtctcctcgt cgatcacgag tccctcgacc tgatggaaca tcggcgtgtg ggtctgatcg
     6601 gagtcgcagc gatagacgcg gcccggcgcg atgacgcgca aaggcggctc gttcgccagc
     6661 atggtgcgga tctgcaccgg gctcgtatgc gtgcgcagca ctggccgcgc gccgtccggc
     6721 ccttcggcga aataaaacgt gtcg
//
LOCUS       DBNHVU010000060        13334 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_987409, whole genome shotgun sequence.
ACCESSION   DBNHVU010000060 DBNHVU010000000
VERSION     DBNHVU010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 13334)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13334)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13334
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_987409"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..736)
                     /locus_tag="AC16Y7_02335"
     CDS             complement(41..736)
                     /locus_tag="AC16Y7_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018236845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5708248.1"
                     /translation="MAVSERRQTRFREMKKNYHRSKISFEFLSPVEKRGAPMSNDTLT
                     AGLESYEIGPKIHRLRKQKGLSLAKLGEHTGMSAGLLSKIERGRLVPTLPTLLKIAMV
                     FGAGLDHFFSDDKDRPVVAVVKKKDRLRLPNRSDGEAPSYFFECLDFPVTDRKMDAYF
                     VEFRPRAKPAEPHQHPGAEVIYVIGGELVVTIDGEETPLREGDSMYFDSSYPHSYRQC
                     GRRKCSAVVVVTS"
     gene            765..1070
                     /locus_tag="AC16Y7_02340"
     CDS             765..1070
                     /locus_tag="AC16Y7_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012354.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR5708249.1"
                     /translation="MEIQTNDWLEPKQFPTVSLNALRAFEVSARHMSMKRAALELNLT
                     PSAISHRLRLLEKTLGCRLFERTGSYLQLTDSGRQLAPALTAAFAQIMEAVGSVRRD"
     gene            2009..4198
                     /locus_tag="AC16Y7_02345"
     CDS             2009..4198
                     /locus_tag="AC16Y7_02345"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024267.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5708250.1"
                     /translation="MLARVLAYSIPASLLPLDAAIAQEAAGFDARTLLTLGINLGIIT
                     FAVGTAIACMRATKRARAQEAKAAGEAERYALSESMLETVLAAEPQALMTLTETGEPE
                     LLVSNLPGSFGVPRDPARLMNFVSWLDSESTAELEPAMQALAERGEPFNLMLRAARGR
                     YVEVDGRTAGRQLVFKVRDTAGRRLELADLSSKHKELEEQVSSLRALLDEAKRNETTR
                     AESKASIEAHFRSFDRLATAFAVFDSGQRLAHYNQAYVDLWQLDPAWLATHPRDGEIL
                     DRLRQARRLEERADYRDWKQSWLSPYGSNQQVEDRWYLPDGRSLHVIADADGKGVTYL
                     YENVTEQIALESRYNALIQVQRETLDTLREGVAVFAPDGRLRLHNSAFARIWQLDPEL
                     VDGEPHVDDVIAQCRKLYDVPEEWERTVAMVTAIVAERHPYESQFDRPDGTVIKSAAL
                     PLPDGDTLLIYRDITDAKRAERALIEKNEALQAADRLKSDFVSHVSYELRTPLNSMLG
                     FAQMLAEPAFGPLADKQREYVNDILSSGTTLRAIVDDILDLATIDAGSIDLKLEPVYV
                     RNVIEAARQGVEERLKQNDVKLEIEIALGVDEIVGDASRITQILYNLLSNAIGFSEPG
                     EIVTLKCARADEMIAFDVEDRGVGIPEEFQQSVFDRFESRTQGSRHRGAGLGLSIVKS
                     LAELHGGTVSLSSAPGQGTRVTVRVPLKHEPDREAPNAPAYQLNRAG"
     gene            4195..5718
                     /gene="tsaE"
                     /locus_tag="AC16Y7_02350"
     CDS             4195..5718
                     /gene="tsaE"
                     /locus_tag="AC16Y7_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR5708251.1"
                     /translation="MSASWRLDVVDLEQLDIFASRVALSLKSGDVVTLSGPLGAGKTT
                     FARALVRHLGVEGEVPSPTFAIMQRYETQRLTLVHCDFYRLQPDELDELGLDDALSEG
                     SLLIEWPERGGDWLPADRLDITLEDAEDDTRRIDLAGHGAWGPRLERLHALSHFLKGT
                     SFAGAEARYLQGDASTRSYARLFGADARAILMNSPRQPDGPPIQDGKPYSALVHLAED
                     VTPFVAVAGALGDCGLSAPQIHAFDLDRGFIVLEDFGDLVFGSEVARGADQGALWRAA
                     VDVLVALADRPPPETLPIPGHAPYRLPRFDADAMLIEASLIIDWLWPALHGAETPPTL
                     RQEFQDLWRPHLEVAAAADPGWVLRDYHSPNLMWLPERTSIQKVGLLDFQDALRGPLA
                     YDLVSLLQDARLDVPEALERDLLDRYCAARSAQSESFDSHDFRRLYAVLGAQRNSKIL
                     GIFARLAKRDGKRGYLAHIPRVARYLERDLAHDALGDLRAFYAREFPASADLAPLAL"
     gene            5746..6477
                     /locus_tag="AC16Y7_02355"
     CDS             5746..6477
                     /locus_tag="AC16Y7_02355"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR5708252.1"
                     /translation="MTIARTAMVLAAGYGARMRPLTETMPKPLVPLLERPLIDHVLDR
                     LAAAGVETAIVNVHYLPEQIEAHMRERASRPPATKISDEGDLLLDTGGGAKKALPMLG
                     DGPFFIHNSDSVWAEGVTPALPAMLRAWDPERMDCLLLLAPLSTSIGYAARGDFDMAP
                     DGRLTRRGERQVVPFAFAGVSLCTAALFEDAPDGPFSLNLLWDKALAKGRLHGARLEG
                     RWMHVGTPEALVEAEASFEHEDEGA"
     gene            6464..9562
                     /gene="addB"
                     /locus_tag="AC16Y7_02360"
     CDS             6464..9562
                     /gene="addB"
                     /locus_tag="AC16Y7_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HIR5708253.1"
                     /translation="MKAPKSLTAPHLYTIPPSAPFLSTLAQAIMSGDLPLPGGTKPDP
                     LTLPQVTIYLPTRRAARALREAFLAQGGGEAMLLPRIHALGQADEDAALILEAEAFSA
                     ADGALGAPAIPTLRRRLALMRLIRAQGAAPAAEATETEAGAARWHLSPGQASSLASDL
                     ADLMDVVESEEIDFSALDALAPDDLAAHWDITVRFLKIVTEHWPQYLEDNGLTSPVTR
                     RNLLMARETARLGRGAPYPVIAAGSTGTVPATARLLGTIANLENGAVVLPGLDRLLDD
                     ESWDSLPEHPEHPQAGMAELLRKLGVTRDAVAYVPGSDPDGAARARLHLVSEALRPAE
                     TTEQWQRYLESDALAPDGRASFANALSGVRLLEAPTAHDEAEAIALLLKSCIEVPGKT
                     AALVTPDRVLARRVAARLKGFGLTVDDSAGEPVARTMPGAFLDLLLGAMETDFAPSAL
                     MALLKHPLTRLGRAPKAIRNAARMLERGAFRDVYLGQGLTGVAHALQTANLRRPPTDA
                     ERDDALGLVADLEQAFAPLVRLFESSTPVSVTTLAEAHAASAEALSRDASGTSAGLWQ
                     GDEGEALSLLLAELIEEGEGLALRPLEYPALYRSLLAGQVVRPRGATHPRLHIWGPLE
                     ARLQQPDVVILGSLNEGIWPRPQESGPWLSRPMRATLGLTPPERRTGLSAHDFAQALG
                     ANSVYLTRALKVDGVPTVPSRWVQRLEALIGASGLQSKLVPEQPWVAWARERDRVERF
                     EPAEPPSPRPPVVARPRQLSVTQIERWIANPYEIYAKHILRLEPLAELGAEPDPAMRG
                     TAFHRILHLFARAHPDALPDDIYGALKSIADAEFDALAHAPSIEAFWRPSFQRFARWF
                     AATEPMRRAEIARTYSETKGSLQVSDGFRLTARADRIDEMESGALVIYDYKTGGLPRQ
                     IHVTELYAPQLPLEAAIAEAGGFAELGPRDVEDLVYIRASGRQEGGDEQAASPTSART
                     LSAKALEDLQRLVAYFEDPATPYDVKRRAGGGFANAYRYDDYEHLARVQEWLTEEAEE
                     GW"
     gene            9562..13104
                     /gene="addA"
                     /locus_tag="AC16Y7_02365"
     CDS             9562..13104
                     /gene="addA"
                     /locus_tag="AC16Y7_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair helicase AddA"
                     /protein_id="HIR5708254.1"
                     /translation="MSDAAHDLLHEADANQARASDPSASAWVSANAGTGKTAVLVKRV
                     LRLLLAGSKPESVLCLTYTKTAAAEMQNRLLKSLADWATMPDQALRDVLAALMRRAPD
                     EEDLRAARRLFAKALEARGGLKIHTIHGFCERLLQRFPLESGVTPHFSVLEEHSAAQL
                     KDEAFDTVVAEAAAAPNATLGLALTKIIGLTSEDYFRQVVNVVLSKRAALSRMVAVHD
                     GLEDWAAAEAEALRQLLGVAGKEEDAILSTQAAILTDEQIDAALAAFSAYGSDAKTDQ
                     QAQGALENARGLDGEARANALRALFLTQKGTPAANLCTKGFKDAAPDIGAMLDAAKGP
                     FAALETDLAHLRVAEASGAVIVLADAILADYEGRKIRNGVLDYDDLIIKAQHLLSRTG
                     AAAWVLYKIDGGVDHILVDEAQDTNPEQWSIIETLADEFFAGRGAGEQTRTLFAVGDE
                     KQSIYSFQGANPARFGAVGQAFSKRAQAAQIKWHKVPLNVSFRSTVPVLAAVDHVFGQ
                     DHAAQGLTFAENTIIEHHAFRQGEAGLVELWPVETTDKPEPAEPFEPWTEDVSGADAV
                     DTLCKRIATQIRRWLDSAEPLPSQGRPIQPGDILILVRRRDPFTAPMIRELKRAKIPI
                     AGADRMRLMDQLAVQDLVALADVLLMPEDDLALAVVLKSPLFGLSEDDLFALAHDRKG
                     SLWAELVARRDEEKFTEAATRLAAWLGHADTLPPYEFFAELLGADGQLMRKRMLTRLG
                     PEAAEAIDEFLDAALAYDRDSTPSLQGFVHELRHGEIDIKRDMDQDRDEVRIMTVHGA
                     KGLQAPIVILPDTCTNPRGQGPRIYEAPRPGQPPGEVDHLLWPPAGHSNVPGLATAKS
                     ATERAERDEYHRLLYVAMTRACDRLYICGWQGVQRRDKNCWYDLVAEGLEPHLSQTDG
                     DDGLTVSRMTSSQKKPAKDAATPATVLETPPLPDFALTPAMPERVRTRLAPSHLALNA
                     ASEGDGNGGSDGAFGPEQPPLGPSALAGDLRFVRGGLVHALLQHLPEVPAAEQERAAR
                     SFVAARGVSLPDTLREEIVGETLAIVRDATFAPLFAPGSLAEVPVVARFGDGEDGFDL
                     EGQIDRLAILDGDLLILDYKTNRPPPTTPGEVAQSYINQLAAYRLALGKMFPGKPARV
                     ALLWTDGPRLMEIPSTLLEAAEHDMLSRGPALTR"
     gene            13177..>13334
                     /locus_tag="AC16Y7_02370"
     CDS             13177..>13334
                     /locus_tag="AC16Y7_02370"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR5708255.1"
                     /translation="MAIDTVSDDTFDAEVLKSSTPVVVDFWAEWCGPCRQIAPALEEI
                     ASELGGKV"
BASE COUNT         2388 a         4309 c         4272 g         2365 t
ORIGIN      
        1 tctcactttc gaggacactg cctagcggat aaaggcgtcg tcagctcgtc accacaacga
       61 ctgcggaaca tttccgtcgg ccgcactgcc ggtagctatg cggataggat gagtcgaaat
      121 acatcgaatc cccctcacga agcggggttt cctcgccatc gatggtcacg acaagctctc
      181 cgcctatgac gtagatcacc tcggctcctg ggtgctggtg gggttctgcc ggctttgcgc
      241 gtggtctaaa ctccacgaaa tacgcgtcca tcttgcgatc ggttacggga aaatcaaggc
      301 attcgaaaaa gtaactcggt gcctctccat cgctgcgatt gggcagccgc aagcggtcct
      361 tcttcttaac gaccgcaacg accgggcgat ccttatcgtc gctgaagaag tgatcgagcc
      421 ccgcgccgaa aaccatcgct atcttgagca gcgtcggaag ggtcggcacc agcctaccgc
      481 gctcgatctt ggacagcagc cccgcagaca tgcctgtgtg ttcaccaagc ttcgccaggc
      541 tgagaccctt ttgcttacga agcctgtgga tcttcggacc gatctcatag ctttccaaac
      601 ctgctgtcag ggtgtcgttg gacatgggcg cgcctctttt ttcgactggt gacaaaaatt
      661 caaaggatat tttacttcta tgataatttt ttttcatttc acgaaatcgt gtctggcgcc
      721 gttcggagac cgccatgtag cctcccctaa caggagtgac acacatggaa attcagacca
      781 acgattggct tgagcccaag cagttcccga ccgtgtccct caatgcactt cgtgcctttg
      841 aggtgtcggc gcgtcatatg tcgatgaaga gggctgcgct cgaactcaac ctgactccat
      901 cggcgattag ccatcggctg cgccttctgg aaaagaccct cgggtgccgg ctctttgagc
      961 gcacgggatc atacctccag ctcacggact ccggtcggca gctcgcgccg gcgctaacgg
     1021 ccgccttcgc ccagattatg gaagccgtgg ggagcgttcg ccgcgattag cgaacagtcc
     1081 caaaaggaga gacctcatta tgttaagaac ccttctcatc agtgctcttg tcgtaactct
     1141 tgctgctcgt gatgcgggca caaatgagca ggtgatctcc gctgacgcag gaaagattgt
     1201 ggcctccctc ttgtcgtatc ctacgggaaa cgccgcccca gcgggttggt ttcatcgcgc
     1261 atcggacaag gcggacgtcc cgcccccaaa caccctcgca gtcattgcgt gccgggttga
     1321 gcgaaagtct gaggccaaag ggggcgccgc cgaaagtgca catcggatcg aatggcgctt
     1381 gcctcttgag tgcatgagag tcgtcgagac agtgcgtgat gccgcagctc tcagcaatct
     1441 gtggctacgt gagcgaaacc ccgatctttc cgaacacagc gtttgcgccg agatcgtaat
     1501 gacctacgca cccatctggg aggccagcca caacaactgg gcgatcgtaa aagtgggctg
     1561 tccgacgaag attgtggacg cgagtggtcg agtcatcggc tggcatatgc ccgagtgcca
     1621 gggcacgatt cctggaacga cgtatccgct tcgctgccgt ttcgacccaa gcgaagtctg
     1681 acgtgcgctc atggacctag tggccaggcc aattagatcc gaggagcgtc aaatttagcc
     1741 acttttgcaa caaagcgggg tttccggccc tgtgggcaag agtccacgaa gcagttagtc
     1801 taaaagtgat tcgcccttgc ggtccgcgga gagccacccg gctccagcat tcaagtctgt
     1861 ggcagcgttc agcggcgcga ggcgatgcca taggcaaatg cgttgttggt gagatgcgtt
     1921 ggctatgacg ttagcggcgg cgtgggagca cggggggacc atgcaaacca gcagaggaga
     1981 ggcgacacct gactcaaggt ggcccaccgt gctggcccgt gtcctggcct attccattcc
     2041 ggcttcattg cttccgctcg atgccgccat cgctcaagaa gcggccgggt tcgacgcgcg
     2101 aacgcttctg acacttggaa tcaatctcgg gatcatcacc ttcgccgtcg gcacggccat
     2161 cgcttgcatg cgcgcgacca agcgcgctcg ggcgcaggaa gccaaggcag caggcgaggc
     2221 ggaacgttac gccctcagcg agagcatgct ggagaccgtg ctcgccgccg agccgcaggc
     2281 gctgatgacc ctgaccgaga ccggagagcc cgagcttctc gtctccaacc tgccggggag
     2341 cttcggcgtt ccgcgcgacc ctgcgcgtct catgaatttc gtatcttggc tggactcaga
     2401 gtcgaccgcg gagctcgagc cggccatgca ggccttggcc gaacgcggcg agcccttcaa
     2461 cctcatgctg cgcgcggcgc ggggccgcta tgtcgaggtc gacggccgta cggcaggccg
     2521 acagctggtg ttcaaggtcc gtgataccgc cggacgacgg cttgaacttg ccgacttgtc
     2581 ttccaaacac aaagagctgg aggagcaagt ctcgtccttg cgcgctttgc tggacgaagc
     2641 gaaacggaac gagaccaccc gcgcggaatc aaaagcaagc atcgaggcgc atttccgcag
     2701 tttcgacagg cttgcgaccg cgttcgccgt gttcgattca ggacagcggc ttgcccacta
     2761 caaccaggcc tatgtggatt tgtggcagct cgatcccgcg tggctggcta cgcacccccg
     2821 cgatggggag atcctcgacc gcctgcgcca ggcgcggcgt ctggaggaac gggcagacta
     2881 tcgcgattgg aaacagagct ggctttctcc ttacggctcc aaccagcagg tcgaggaccg
     2941 ctggtacctg ccggacggac gctcgctgca tgtgatcgcc gatgccgacg gcaagggcgt
     3001 gacctatctc tatgagaacg tcaccgagca gatcgcactg gagagccgct acaacgcgct
     3061 gatccaagtt cagcgggaga cgctcgatac tttgcgagaa ggtgtcgccg tgttcgctcc
     3121 cgacggacgt ttgcggcttc acaacagcgc cttcgccaga atctggcaac tcgatccgga
     3181 attggtcgat ggcgaaccgc acgtggacga cgtcattgcc caatgccgca agctctacga
     3241 cgtgccggag gagtgggagc gcaccgtcgc aatggtgaca gcgattgtcg ccgagcggca
     3301 tccttatgaa agccaattcg accggcccga tggcacggtg atcaagtctg ctgcattgcc
     3361 gctgccggac ggcgacacac tgctgatcta ccgggacatc accgatgcca agcgcgccga
     3421 gcgcgccttg atcgagaaga acgaggcgct gcaggcggcg gaccggctca agagcgactt
     3481 cgtttcacac gtttcctacg agttgcggac accgcttaac agtatgctgg gctttgcgca
     3541 aatgctcgcc gagcctgctt tcggcccgct ggctgataag cagcgcgagt atgtgaacga
     3601 tattctgtcg tcgggaacga cgctgcgcgc catcgtcgac gacattctcg acctggccac
     3661 gatcgacgcg ggctcgatcg atctcaagct tgagccggtc tatgtgcgca atgtcatcga
     3721 agccgctcgc cagggtgtgg aagaacgcct gaaacagaac gacgtgaagc tcgagatcga
     3781 gatcgcactc ggtgtcgacg agatcgtggg cgatgctagc cgcatcacgc agatcctcta
     3841 taatctcttg tccaacgcca tcggcttttc ggagccgggc gagatcgtga cgctcaaatg
     3901 cgcccgcgcc gacgagatga tcgccttcga cgtcgaggac cgcggcgtgg gcattccaga
     3961 ggagttccag cagtccgtgt tcgaccgttt cgaaagccgc acgcaaggct cgcgccatcg
     4021 cggcgctggc ttgggactat ccatcgtcaa gagcctagcg gagctccacg gcggtaccgt
     4081 gtcgctctca tcggcaccgg gccaaggcac aagggttacg gtccgcgtgc cactcaagca
     4141 tgagccggat cgggaggcgc ctaacgcgcc agcctatcag ctcaaccgcg ccggatgagc
     4201 gcaagctggc ggctcgacgt tgtcgacctg gaacagctcg acatttttgc cagccgggtc
     4261 gcactttccc tcaagtctgg tgacgtcgtc acgctgagcg gaccgctcgg cgcgggcaag
     4321 accacgtttg cgcgcgcgct tgtgcgccac cttggcgtcg agggcgaagt gccaagcccc
     4381 accttcgcga tcatgcagcg ttacgaaacc cagcggctga cattggtcca ttgcgacttc
     4441 tatcggctcc agccggacga gctggacgag ctcgggctcg acgatgcgct ttccgaaggc
     4501 agcctcctga tcgaatggcc ggagcgcggt ggcgattggc ttcccgccga ccggctcgac
     4561 attacgcttg aggacgcgga ggacgatacc cgccgcatcg acttggccgg acatggtgct
     4621 tggggcccgc gcttggagcg cctgcacgcg ctgtcgcatt ttctgaaggg cacgtcgttc
     4681 gcgggcgccg aggcgcgcta cctgcaaggc gatgcatcca cgcgcagcta cgcgcggctt
     4741 tttggtgccg acgcccgcgc cattctgatg aattcgccgc gccagccgga tggtcccccg
     4801 atccaggacg gcaagccgta tagcgcactg gtccatttgg cagaagacgt gacaccattc
     4861 gtggccgttg ccggcgcgct cggcgactgc ggtctttcgg cgccacagat ccatgctttc
     4921 gacctggacc gagggtttat cgtcctggag gatttcggcg atctcgtctt tgggtccgag
     4981 gtcgcgcggg gtgcagacca aggtgcgttg tggcgcgccg ccgtggacgt tctcgtggcg
     5041 ctggccgata ggccgccgcc ggagaccttg cccattccgg gccacgcccc ctatcgtctg
     5101 ccgcgcttcg acgcggacgc catgttgatc gaggcctctc tgatcatcga ctggctgtgg
     5161 ccggcgctcc atggcgccga gacgcctccg acgctgcgcc aggagttcca ggacctctgg
     5221 cggccgcatc tggaggtagc cgccgccgcg gatccaggct gggtcttgag ggattatcac
     5281 tcgcccaacc tgatgtggtt gccggagcgg acaagcatcc agaaggtcgg cctcctggac
     5341 ttccaggatg cgctccgcgg ccccctcgcc tacgacttgg tctccctcct ccaggatgcc
     5401 cggctggacg tgcccgaggc gcttgaacgc gatctgctcg accggtactg tgccgcccga
     5461 agcgcgcaga gcgagtcgtt tgatagccat gatttccgca ggctctacgc agttctcggc
     5521 gctcaacgca actcgaagat cctcggcatt tttgcgcgat tggcgaaacg tgacggtaag
     5581 cgcggctatc ttgcccacat cccgcgggtg gcgcgctatc tcgagcgcga cctggcgcac
     5641 gacgcgctcg gcgacctgcg cgccttctat gcgcgcgagt tccccgcaag cgccgacctg
     5701 gcgcccttgg ccctttagct cttggcgctc taaaaggacg tggacatgac catcgcacgc
     5761 acggccatgg tgctggcagc cggctatggc gcgcggatgc ggcctctcac ggagaccatg
     5821 ccgaagccgc tggtgccgct gctggagcgc ccgctgatcg accacgtcct ggaccgcctc
     5881 gctgcggcgg gcgtggagac ggcgatcgtc aatgttcatt acctgcccga gcagatcgaa
     5941 gcgcatatgc gtgagcgcgc gagccgcccg ccggcaacca agatttccga cgagggcgac
     6001 cttctgctcg acacgggggg cggtgcgaag aaggcgctgc caatgctcgg agacggaccg
     6061 ttcttcatcc acaactccga ctctgtgtgg gccgagggcg taacgccggc gctgcccgcc
     6121 atgctgcggg catgggaccc cgaacgcatg gactgcctcc tgttgctcgc gccgctttcc
     6181 accagcatcg gctatgcggc acgcggcgat ttcgacatgg cgcccgatgg acgcctgaca
     6241 cggcgcgggg aacggcaagt cgtgccgttc gcctttgccg gcgtatctct gtgcacggcc
     6301 gcattgttcg aggacgctcc ggacgggccg ttctcgctca acctgctgtg ggacaaagcg
     6361 ctcgccaaag gccggctcca tggcgcgcgt cttgagggac gctggatgca tgtgggcacg
     6421 ccggaggcgc tagtggaggc cgaagcctcg ttcgagcacg aagatgaagg cgcctaagtc
     6481 tctcacggcc ccccacctct acacgatccc gccttcggcg ccgtttctgt cgaccctcgc
     6541 ccaggcgatc atgtccggcg atttgccgct tcctggcgga accaagccag acccgctcac
     6601 gctgccgcaa gtcacgatct acctgccgac gcggcgtgcg gcgcgcgctt tgcgcgaggc
     6661 ttttcttgcg caaggcgggg gcgaggcgat gctgctgccg cgtatccacg cactcggaca
     6721 ggccgacgag gacgccgccc tgatccttga ggcggaggcc ttcagcgcgg cggacggcgc
     6781 actcggcgca ccggcaattc ccaccttgcg gcgccggctg gccttgatgc ggttgatccg
     6841 cgcgcaaggg gccgcacctg ccgcggaagc cacggagacg gaggccggcg cggcgcgatg
     6901 gcacttgagc ccaggacagg cgtcaagcct cgccagcgat ctggccgatc tgatggatgt
     6961 cgttgagtcg gaggagatcg acttctccgc gctcgatgcg ctggcgcccg atgacctcgc
     7021 tgcgcattgg gacattaccg tccgcttcct gaagattgtc accgagcact ggccgcaata
     7081 tctggaagac aacggcctca cctcgccggt cacgcgccgc aatctgctga tggcgcgaga
     7141 gacggcgcgc ctgggacgcg gcgcgcccta tccggtcatt gccgcgggct ccacgggcac
     7201 ggttcccgcc accgcgcggc tgctcggaac catcgccaat ctggagaacg gcgccgtcgt
     7261 gctgcccggg ctcgatcgtc ttctcgacga tgagagctgg gacagtctgc cggagcatcc
     7321 ggaacatccc caagcaggca tggccgaact gttgcgcaag ctcggcgtga cgcgcgacgc
     7381 ggtcgcttat gtacccggca gcgacccgga cggcgccgcc cgcgcgcgcc tgcatctggt
     7441 gagcgaagcg ttgcgtcccg ccgagacgac cgaacagtgg cagcgctacc ttgagagtga
     7501 cgcgctcgca ccggatggcc gcgcgagctt tgccaatgcg ttatccggtg tgcgcctgct
     7561 tgaagcaccg acagcccatg acgaggccga agcgatcgcg cttctcctga agtcgtgcat
     7621 tgaagtgccc ggcaagacgg cggcgctggt cacgcctgac cgtgttcttg cgcgccgggt
     7681 ggccgcgcgg ctcaaaggct tcggtctcac cgtggacgac tcggccggtg agcccgtcgc
     7741 acggaccatg ccgggcgcgt ttctcgatct tctgctcggt gcaatggaga cggacttcgc
     7801 accatctgcg cttatggcgc tgctgaaaca tccgttgacg cgattggggc gcgcgcccaa
     7861 ggcaatccgc aacgcggcgc gcatgctcga gcgtggggca tttcgtgacg tctatcttgg
     7921 ccaagggctg acaggcgtgg cgcacgcctt gcaaactgcg aatcttcgca ggccgccaac
     7981 ggacgcagag cgcgacgatg cgctgggtct tgtcgcggat ctagagcaag cctttgcgcc
     8041 gttggtccgg ctctttgagt cctccacccc ggtaagcgtg acaacgctcg ccgaggccca
     8101 tgcggcaagc gcggaggcgc tgtcacgcga tgcaagcgga acgtcggcgg ggctgtggca
     8161 aggcgatgag ggcgaagctc tcagcctgct tcttgccgag ctcatcgaag agggagaagg
     8221 gctcgcgttg cgcccgcttg agtatccggc cctctaccgc agcctgctcg cgggccaggt
     8281 ggtgcgcccg cgcggggcga cccatccgcg gcttcacatc tggggtccgc tcgaagcgcg
     8341 gctgcagcag cccgacgtcg tcattctcgg cagcctgaat gaaggcatct ggccgcggcc
     8401 ccaagaatca gggccttggc tcagccgccc gatgcgcgcc acgctcggcc tgacaccgcc
     8461 ggagcggcgc acgggcctga gcgcccacga cttcgcccag gcgctgggcg ccaattccgt
     8521 ctacctcacc cgcgcgctca aagtggacgg cgtgcccacc gtgccctcgc gctgggtgca
     8581 gcggcttgag gctttgatcg gcgcatcggg gcttcaatcg aaacttgtgc cggagcagcc
     8641 ttgggttgcc tgggcgcgcg agcgcgatcg cgtcgaacgg ttcgagcccg ccgagccgcc
     8701 gagcccccgc ccgcccgtcg tggcgcggcc gcgccagctt tccgtgacgc agatcgaacg
     8761 ttggatcgcc aatccttacg agatctacgc caagcacatc ttgcggctgg agccgcttgc
     8821 cgaactcggt gcggaaccgg accccgccat gcgcgggacc gccttccacc gcatcttgca
     8881 tctgtttgcg cgcgcgcacc cggatgctct gccggacgac atttatggcg cgctcaagtc
     8941 gatcgccgat gctgagttcg atgcgctggc gcacgcgccg agcatcgagg ccttctggcg
     9001 cccgagcttc cagcgcttcg ctcgctggtt tgccgcaacg gagccaatgc gacgcgcgga
     9061 gattgcccgc acgtattcgg agacgaaggg ctcgcttcaa gtctctgacg gttttcggct
     9121 gacggcacgc gcggaccgca tcgacgagat ggaaagcggc gcgctcgtca tctacgacta
     9181 caagaccggc gggctgccgc gccagatcca cgtgaccgaa ctctacgcgc cgcaattgcc
     9241 gctggaggct gccattgccg aagccggtgg atttgccgaa ctcggaccgc gcgacgtgga
     9301 agacctcgtc tatatccgcg cgtccggacg ccaagagggc ggtgacgagc aggccgccag
     9361 ccccacgtca gcccgcacct tgtccgccaa ggcgctagag gatctgcaac gcctcgtcgc
     9421 ctatttcgag gatcccgcca cgccctacga cgtcaagcgc cgcgcgggcg gaggcttcgc
     9481 caatgcctac cgctatgacg actacgagca cttggcgcgc gtacaagaat ggctgaccga
     9541 agaagccgag gagggttggt gatgagcgac gctgctcacg acctcctcca cgaagcggac
     9601 gccaatcagg cgcgcgcgtc agacccgagc gcatcggcct gggtgtcggc caatgcgggc
     9661 acgggcaaga ctgccgtcct ggtgaagcgc gtcttgcgtt tgctgctcgc ggggtcaaag
     9721 cccgaaagcg ttctgtgcct cacctacacg aagaccgcgg cggcggagat gcagaaccgc
     9781 ctgctcaaga gcttggcgga ctgggcgaca atgcctgacc aggccttgcg cgacgtgctt
     9841 gccgcactca tgcgccgcgc gccggacgag gaggatttgc gtgccgcacg caggctcttt
     9901 gccaaggctc tcgaagcgcg cggcggtctc aagatccaca ccattcacgg attctgcgag
     9961 cgcttgctgc agcgatttcc tttggagtcc ggcgtgacgc cgcatttctc cgtgctcgaa
    10021 gagcacagcg cggcgcagct taaagacgag gctttcgaca ccgtcgtggc ggaggccgcg
    10081 gccgcaccca atgccacgct cggcctggcg cttaccaaga tcatcgggct gaccagcgag
    10141 gactatttcc gccaagtggt gaatgtcgtt ctgtcgaagc gcgcggcgct gtcgcgcatg
    10201 gtcgcggtgc acgatggcct agaggattgg gcggccgccg aagctgaggc cttgcggcag
    10261 ctattgggcg tggcgggcaa ggaagaagac gcgattcttt ccacgcaagc cgcgatcctg
    10321 acggacgagc agatcgacgc ggccttggcc gccttctccg cttacggcag tgacgccaag
    10381 acagaccagc aagcgcaagg cgcgctggaa aatgcgcgag ggctcgatgg cgaggcgcgg
    10441 gcaaacgcac tacgcgctct gttcctcacg cagaagggca cgcctgccgc caatctctgc
    10501 accaaaggct tcaaggatgc cgcgcccgat atcggcgcga tgctcgacgc cgctaaaggc
    10561 ccgttcgccg cgctggagac ggacttggcg caccttcgcg tggccgaagc gagcggcgcg
    10621 gtcatcgtgc tcgccgatgc gatcctggcg gactacgaag gccgaaagat tcgcaacggg
    10681 gtgctcgact acgacgactt gatcatcaag gcacagcatc tactgtcgcg cacaggtgcg
    10741 gcggcctggg tgctctacaa gatcgacggg ggtgtcgacc atatcctggt ggacgaggcg
    10801 caagacacga accccgagca atggtcgatc atcgagacgc tggcggatga gttcttcgcc
    10861 gggcgcggcg ccggcgagca aacgcgcacg cttttcgccg tgggcgacga gaagcaatcg
    10921 atctacagct ttcagggcgc caatccggcg cgcttcggcg ccgtgggcca ggctttcagc
    10981 aaacgcgcgc aagccgcgca gatcaaatgg cacaaggtgc cgctcaacgt gtcgttccgc
    11041 tccacggttc ccgtgctagc tgcggtggat catgtgttcg ggcaggacca cgcggcgcaa
    11101 gggctgacct tcgccgagaa caccatcatc gagcatcacg ccttccgcca gggcgaggcg
    11161 gggctcgtgg agctttggcc ggtcgaaacg acggacaagc cggagcccgc cgagccgttc
    11221 gagccctgga ccgaggacgt cagtggcgcg gatgcggtcg atacgctgtg caaacgcatc
    11281 gcaactcaga ttcgccgctg gctggatagt gctgagcccc tgccctcgca aggacgcccc
    11341 attcagcccg gcgacattct gatcttggtg cggcggcgcg atcccttcac cgcgccaatg
    11401 atccgcgagc tcaagcgggc aaagattccc atcgccggcg ccgaccgcat gcggctgatg
    11461 gatcagctag ccgtgcagga tctcgtggcg cttgccgacg tgctgcttat gccggaggac
    11521 gacttggcgc tcgcagtggt gctgaagagc ccgctattcg gcctcagcga agacgatctg
    11581 ttcgcgctgg cgcacgatcg caaaggatcg ctgtgggcgg agctcgtggc gcggcgggac
    11641 gaggagaaat tcaccgaagc tgcaacgcgg cttgccgcct ggctcggtca cgcggacacc
    11701 ctgccacctt acgaattctt cgccgagctg ctcggggccg acggccagtt gatgcgcaag
    11761 cgcatgctga cgcggttggg gccggaggcg gccgaagcga tcgatgagtt tctcgacgcc
    11821 gccctcgcct acgaccggga ctccacgccg tcgctgcaag gcttcgttca cgaactgcgc
    11881 catggcgaga tcgacatcaa gcgagacatg gatcaagacc gcgacgaggt gcgcatcatg
    11941 accgtgcatg gcgccaaagg cttgcaagcg ccgatcgtca tcttgccgga cacgtgcacg
    12001 aacccgcgcg gtcaaggccc gcgcatctat gaagcgccgc gccccggaca gccgcccggt
    12061 gaggtggacc accttttgtg gccgccggcg gggcattcca acgtgcctgg gctggcgacg
    12121 gctaaaagcg ccacggagag agccgaacgc gacgaatatc accggctgct ctacgtcgcc
    12181 atgacgcgtg cctgcgaccg gctctatatc tgcggctggc agggcgtaca gcgccgcgac
    12241 aagaactgtt ggtacgatct cgtcgctgag ggccttgagc ctcacctcag ccaaaccgat
    12301 ggtgacgacg gcctcaccgt ttcccgcatg acctcatcgc agaaaaaacc cgccaaggac
    12361 gcagccacgc cggccaccgt gctggagacg ccaccgctgc cggacttcgc cctgacgccc
    12421 gcgatgccgg aacgcgtgcg cacacggctc gcgccgtcgc atcttgcgct aaatgccgca
    12481 agtgaaggcg atggcaatgg cggtagtgat ggcgcgttcg gtccggagca gccgccgctt
    12541 gggccaagcg cgctggccgg cgatctgcgt ttcgtgcgcg gaggcctcgt gcatgcgctg
    12601 cttcagcacc tgcccgaggt acctgcggca gagcaggagc gtgcggcgcg tagcttcgtg
    12661 gcggcccgcg gcgtaagcct gcccgatacg ctaagggagg agatcgtcgg cgagacgctc
    12721 gccattgtga gagatgcgac gttcgcgcct ctgttcgcgc ccggaagcct tgccgaagtc
    12781 cccgtagtgg cgcgattcgg cgacggcgaa gatggtttcg atctcgaagg ccagatcgac
    12841 cggctcgcca ttttggacgg cgacctcctg atcctcgact acaagacgaa ccgtccgccg
    12901 cccacaacgc ccggtgaggt ggcgcaaagc tatatcaacc agctcgccgc ctaccggctg
    12961 gcgctcggca agatgtttcc gggaaagccg gcacgcgtgg ccttgctgtg gaccgacggg
    13021 ccgcggctca tggagatccc atcaactttg cttgaggccg ccgagcacga catgttgagc
    13081 cgcgggccag ccttgacgcg ttgagggggc gcacctaggt tccggtggct agatcgggcg
    13141 caaaactggc ccagccggcg gttaggagaa aagcgaatgg cgattgatac ggtttccgac
    13201 gatacattcg acgccgaggt gctcaagtcc tcgacacctg ttgtcgtcga cttttgggcc
    13261 gagtggtgcg gcccctgccg ccagatcgcg cccgcgctgg aagaaatcgc ctccgaactc
    13321 ggcggcaaag tgaa
//
LOCUS       DBNHVU010000061         7890 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1212061, whole genome shotgun sequence.
ACCESSION   DBNHVU010000061 DBNHVU010000000
VERSION     DBNHVU010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7890)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1212061"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="AC16Y7_02375"
     CDS             complement(<1..840)
                     /locus_tag="AC16Y7_02375"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR5708256.1"
                     /translation="MTFLEDLDRTAWAVEQRLGEILAARDTTPPGGAELAEAMRYGAL
                     GGGKRLRPFLLIQSAGLFGVPQASALDTACALECVHCYSLIHDDLPAMDDDDLRRGRP
                     TLHVAFDEATAILAGDALLTLAFEIVSNPQTCEDAETRAELIHVLAKASGWRGMALGQ
                     ALDLAAERRAFTPEETASMQALKTGALFRFACEAGAMLGRADASCRDALLAYAKAFGQ
                     AFQLADDLLDAQGDAEAMGKAVAKDASRCKATLVALLGIDGATAHLAGLVAEAENALK
                     PFGE"
     gene            993..1679
                     /gene="mtgA"
                     /locus_tag="AC16Y7_02380"
     CDS             993..1679
                     /gene="mtgA"
                     /locus_tag="AC16Y7_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014283146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HIR5708257.1"
                     /translation="MDGRWRLLLARLGLAAAILAGAVLVSIVLFRFVNPPITATMVVD
                     KLRGGTLHRQWVPLEKISPNLQLAVIASEDGNFCDHRGVDWGAVREVINKAKSLKAVR
                     GASTIPMQVAKNLYLWERRNYVRKALEVPLAYAMTTLWPKRRMLEVYLNIAQWGPHTY
                     GAEAASRRYFGKPASRLTRRQAVLLAAALPNPHARNPARPNRKMRLIASAVQRRLPYI
                     SKRADCVLKP"
     gene            1782..1967
                     /gene="rpmF"
                     /locus_tag="AC16Y7_02385"
     CDS             1782..1967
                     /gene="rpmF"
                     /locus_tag="AC16Y7_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR5708258.1"
                     /translation="MAVPKRKVSRMKRGNRRAHDAVQSPAYVEDKDSGELRRPHHIDL
                     KSGRYRSRQVIVIKEEA"
     gene            2253..4037
                     /locus_tag="AC16Y7_02390"
     CDS             2253..4037
                     /locus_tag="AC16Y7_02390"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR5708259.1"
                     /translation="MVESSNKVSAGGAMPSSESAGDVSGPDACTESGAPLDIAQVLES
                     VSATAYRWDFNTDTIEWADNAGTKLEVADMGKLGKGRAFALHIDPEHAGARYDGVTGA
                     PRAEPGAARRYALHYRFLPDGRRGKPALWVEDTGVCFTDADGRPVQAQGTLRVMEDRR
                     QEDRTQPASGHDELTGQLNRARITEALGDLLSNTSRSPANGAFLLAGVNDLTLINETY
                     GFDVGDEVITIVGKRLARVLRGKDCIGRFSSNKFGLVLHNCKAEGVEAIARRLMAAIG
                     DSILDTSVGAVAATISIGAVILPDDATGAQSAVGRALSALDVARVNRGDQFSFYVPCE
                     RKETERKQNVAIADDIIRALNDRRMMIALQPIVTSRSHEPELYECLLRMRRMDGSVVS
                     AGEFIPVAEQFGLAKLIDHRVLELAIELLRTMPKVKLALNVSAATTTNPEWISALESL
                     TGKDRSILERITIEITETTAISDVKAAIEFVSSLKTLGCRVALDDFGAGYTSFRSLRD
                     LGVDMVKIDGSFIENLGTDAEDEMFVRTLIDLARSFGVVTVGEWVGDERTVQLLEKAG
                     VSYMQGYFFGAPELTKDEDGREAQKPAS"
     gene            complement(4034..4564)
                     /locus_tag="AC16Y7_02395"
     CDS             complement(4034..4564)
                     /locus_tag="AC16Y7_02395"
                     /EC_number="4.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HIR5708260.1"
                     /translation="MSEFWVFGYGSLMWRPGFPFEAQLPGILDGSHRALCVYSVLHRG
                     TREEPGLVLGLDHGGACQGVAFRVTPGAEDDTVAYLREREQVTDVYVEAYRDVRLDDG
                     SNRMVTALTYLADCNHAQYAGPLSLEEQLRIVLRCSGQEGANIEYVLNTVQHLEELGV
                     HDDMLANLATLLRREA"
     gene            complement(4573..5499)
                     /locus_tag="AC16Y7_02400"
     CDS             complement(4573..5499)
                     /locus_tag="AC16Y7_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
                     /protein_id="HIR5708261.1"
                     /translation="MTKPLFDTLAIIGPGLIGSSIMRAARAHDAVRTIVTCSRSPETR
                     KRVAELGIADKVADSNASAVENADLVIVCTPVGICGAVAEEIAPHLKEGAIVSDVGSV
                     KSAIVRDMSPHLPDNVHFVPAHPVAGTEHSGPDAGFAKLFDGRWCILTPPEGTDPQAV
                     DRLAAFWALFGANVEIMTPDHHDLVLAVTSHLPHLIAYTIVGTADELETVTRSEVLKF
                     SAGGFRDFTRIAASDPTMWRDVFLTNKDAVLEMLGRFNEDVAALTKAIRRGDGDALFK
                     HFTRTRAIRRGVIDAGQDTPDADFGRPHGHED"
     gene            complement(5496..6620)
                     /gene="hisC"
                     /locus_tag="AC16Y7_02405"
     CDS             complement(5496..6620)
                     /gene="hisC"
                     /locus_tag="AC16Y7_02405"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 -
                     L-histidinol-phosphate:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR5708262.1"
                     /translation="MAPKSLAAKGGPAPRPGILDIAPYVPGTRTLPGAGTVVKLSSNE
                     TPLGPSPKAVEAYSAEASCLSRYPDGSAAPLREALAARYGLDAARIVCGAGSDELLNL
                     LACAYLGPGDEAIYTEHGFLVYRIAIQARGATPVVAAETDLTANVDAILARVTEKTRM
                     VFIANPNNPTGTYVPFDEIKRLHAGLPERVILVLDAAYAEYVRANDYEAGIELVATSP
                     NAVMTRTFSKIYGLAALRLGWAYCPAPIADALNRIRGPFNVTGPSIAVGVAALNDVAH
                     MELAVAHNTHWRIVMTEALSGIGLRVTPSVANFLLVHFPDAPGKSARDADKFLHARRI
                     VLRRVEEYGFPNALRMTIGTKEENMAVLDALTEFMGTEAA"
     gene            6810..>7890
                     /gene="metX"
                     /locus_tag="AC16Y7_02410"
     CDS             6810..>7890
                     /gene="metX"
                     /locus_tag="AC16Y7_02410"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase MetX"
                     /protein_id="HIR5708263.1"
                     /translation="MNETPGAERFADLKRREQTTNPQSEIVHFPASEPLPLDCGGALA
                     PLSIAYQTYGTLNAEKSNLILICHALTGDQHAANLHPITGKPGWWDTMVGPGKSIDTD
                     RYFVLCPNVIGGCMGTTGPSSINPETGRAYGPDFPVITVRDMVRAQARLLDHLGIDQV
                     LSVIGGSMGGMQVLEWAATYQDRVFSAIPIACAARHSSQNIAFHEVGRQAVMADPEWC
                     EGRYLDEGKSPRKGLAVARMAAHITYLSDLALHDKFGRNLQDREKVTFGFDADFQVES
                     YLRHQGMSFVDRFDANSYLYITRAMDYFDIAADHDGVLANAFAGTKTRFCIVSFTSDW
                     LFPTEESRDVVHALNAVAANVSFVEI"
BASE COUNT         1445 a         2510 c         2538 g         1397 t
ORIGIN      
        1 ctcgccgaag ggcttgagcg cattctcggc ttcagcgacg aggcctgcga gatgcgcggt
       61 ggcgccgtcg atgccgagca acgccaccag ggttgccttg cagcggctgg catccttggc
      121 caccgccttg cccattgcct ctgcgtcccc ttgcgcatcg agcaggtcat cggcgagctg
      181 gaaggcttgg ccgaacgcct tggcgtaggc cagaagcgca tcgcggcagc tcgcgtccgc
      241 ccggcccagc atggcgcctg cctcgcaggc gaagcgaaat agtgcgccgg tcttcagcgc
      301 ctgcatcgaa gcggtttcct ccggcgtaaa cgcgcgccgc tcggcggcca aatcgagcgc
      361 ctggcccaac gccatgccgc gccagccgct cgccttggcc agcacatgga tgagctcggc
      421 gcgggtttcc gcatcctcgc aggtttgcgg gttcgacacg atttcgaagg cgagggtcag
      481 cagcgcatcg cccgccagaa tggccgtcgc ctcgtcgaaa gcgacgtgca gtgtggggcg
      541 gccacggcgc aggtcgtcgt cgtccatggc gggcaagtcg tcatggatga gcgaatagca
      601 atgcacgcat tcgagcgcgc aagcggtgtc gagcgcggac gcttgcggaa cgccgaacag
      661 gcctgccgac tgaatcagca gaaaggggcg cagacgcttg cctccgccca gcgcgccata
      721 gcgcatggct tcggcgagct cggcgcctcc cggcggggtc gtgtcgcgcg cggcgaggat
      781 ttcgccgaga cgctgctcca cagcccaagc ggtgcggtca aggtcttcga gaaacgtcat
      841 tgccgaaggg atagcggagg cgggccaggg ccgcaaaccg ccggtgcaaa tccaggcctc
      901 cgcctgttgc ccgacgccgt tccggattgg tagaacccgg tcccatggcg gcatcggcgg
      961 gttcaggggc tcagaaacgc agcaagaagc gcatggacgg cagatggcgc ctattgctgg
     1021 cccggttagg actcgccgcc gccattcttg cgggcgcggt gctcgtctcg atcgtgctgt
     1081 tccgcttcgt caatccgccg atcacagcca ccatggtcgt ggacaagctg cgcggcggga
     1141 cgctgcaccg ccaatgggtg ccgctcgaga agatctcccc caatcttcag cttgcggtga
     1201 tcgccagcga agacggcaat ttttgcgatc atcgcggggt cgattggggc gcggtgcggg
     1261 aagtcatcaa caaggccaag agcctgaagg cggtgcgcgg cgccagcacc atccccatgc
     1321 aggttgccaa gaacctctat ctttgggagc ggcgcaacta tgtccgcaag gcgctggagg
     1381 tgccgctggc ttacgccatg accactctct ggcccaaacg gcggatgctg gaggtctatc
     1441 tcaacatcgc ccaatggggc ccccatacgt atggcgccga ggccgcgagc cggcggtatt
     1501 tcggcaagcc cgcctcacgc ctgacgcggc gccaggccgt cctgctggcg gcggccctgc
     1561 ccaacccgca tgcccgcaat ccggcccgtc cgaaccgcaa aatgcgcctg atcgccagtg
     1621 ccgttcagcg gcgcctgccc tatatctcca agcgggcaga ctgcgtcctg aagccctaaa
     1681 aaggtgggtt atcgccactg gccaccgggc tcgattgcca ctataagggc gccacgacag
     1741 gcctcccgca cgatgcggca ggcgcccaga ggtttagaat aatggctgtt cccaagcgaa
     1801 aagtgtcccg catgaagcgt ggcaatcggc gcgcccatga cgcggtgcaa tcgccggctt
     1861 acgtcgagga caaggactcc ggcgagcttc gccggccgca ccacatcgac ctgaagtccg
     1921 gccgctaccg tagtcgtcag gtgatcgtca tcaaggaaga ggcataaggg ccgaggtcac
     1981 caagaccggg ttcttttgcg gttttgatcg ctccgggctt cttgagccgg agtgctttcg
     2041 tttgtctgcc acacttcggt ccctcaaccg tttgtcaacc aattcgcacg aaaggcgctg
     2101 ccttcgcgaa cgcccggctg aagggctggt tttcggcttt cttaagcctc ttcgcttagt
     2161 ctcccggtca acgatcaggc acctaaaaag gtgctccatg cgcaggtcca ttggccacgc
     2221 agcgtgggtc aaaggggcta agggggcgtc gagtggttga gtcttcaaac aaagtttctg
     2281 cgggcggcgc catgccgtcg agcgagtctg ccggagacgt ctccggcccg gacgcctgca
     2341 ctgagagcgg cgccccgctg gatatcgcac aagtcctgga gagcgtgagc gccaccgcct
     2401 accgttggga tttcaacacc gacacgatcg agtgggcgga caacgccgga accaagctgg
     2461 aggtggccga catgggcaag ctcggcaagg gccgcgcctt cgctctacat atcgatccgg
     2521 agcatgccgg cgcacgctat gacggggtca cgggtgcgcc acgtgccgag ccgggtgccg
     2581 cgcgacgcta tgcgcttcac taccgatttt tgcccgacgg gcgccgcggc aaaccggcgc
     2641 tttgggtcga agataccggc gtttgcttta ccgatgccga cgggcggccc gtccaggcgc
     2701 aaggcacgct gagggtcatg gaagatcgcc gccaggaaga tcgcacacag cccgcttccg
     2761 gccacgacga gcttacaggt cagctgaacc gtgcccgcat caccgaagcg cttggcgacc
     2821 ttctatccaa cacctcacga tcgcctgcca acggggcctt tttgcttgcg ggcgtcaacg
     2881 accttacatt gatcaacgag acctatggct tcgacgtcgg cgacgaggtg atcaccattg
     2941 tcggcaagcg gctggcccgc gtgctgcgcg gcaaggactg catcggccgg ttctcgtcca
     3001 acaagttcgg ccttgtcctg cacaactgca aggcggaagg cgtggaggcc atcgcgcggc
     3061 gcctcatggc ggcgatcggc gacagcatcc tggacacgag cgtgggcgcg gtggctgcga
     3121 cgatttcgat tggcgcggtg atcctgccgg acgatgccac cggcgcgcag agcgccgttg
     3181 gacgcgcgct gagcgcgctg gatgtcgcgc gggtcaatcg cggcgaccag ttcagcttct
     3241 acgttccgtg cgagcgcaag gaaaccgagc gcaaacaaaa cgtcgccatt gccgacgaca
     3301 tcatccgcgc gctcaacgat cgccgcatga tgatcgcgct tcagcccatc gtgacctcgc
     3361 ggagccacga gcccgagcta tacgaatgcc tcctacgcat gcggcgcatg gatggttcgg
     3421 tggtgagcgc gggtgagttc attcccgtgg ccgagcagtt tggcctcgcc aagctcatcg
     3481 accatcgcgt tctggaactc gccatcgaac tcctgcgtac catgccgaag gtcaagcttg
     3541 cgctgaacgt gtctgccgcc acgacgacaa atccggagtg gatctcggcg cttgagagcc
     3601 tcacgggcaa agaccggtcc atcctcgaac ggattaccat cgagatcacg gagacgacgg
     3661 ccatttccga cgtgaaggcc gccatcgagt ttgtgtcctc gctgaagacg ttggggtgcc
     3721 gcgtggcgct cgacgatttc ggcgcgggct acacctcgtt ccgctccttg cgggacctcg
     3781 gtgtcgacat ggtcaagatc gatggctcgt tcatcgaaaa tctcggcacg gacgcggaag
     3841 acgagatgtt cgtgcgcacg ctgatagacc tggcccgaag cttcggtgtt gtcaccgttg
     3901 gcgaatgggt cggcgatgaa cggaccgtgc aattgctcga aaaggccggt gtctcgtaca
     3961 tgcaaggcta tttcttcggc gcaccggaac tcaccaagga cgaggacggc cgcgaggcgc
     4021 agaagcccgc gagctaagct tcgcgcctga gaagcgtggc gaggtttgcg agcatgtcgt
     4081 cgtgcacgcc cagctcctca agatgctgca cggtgttcag cacatactcg atattggcgc
     4141 cttcttgccc gctgcatctg agcacaatgc gcagctgttc ctcaagcgag agcgggccgg
     4201 cgtattgcgc gtggttgcag tcagccagat aggtcagcgc tgtaaccata cggtttgagc
     4261 cgtcatctag gcgcacgtcc cgataagcct cgacatagac atcagtgacc tgttcgcgct
     4321 cgcgcaaata agcaacggtg tcatcctccg cgcccggcgt cacgcgaaag gcaacgccct
     4381 ggcaggcacc gccgtggtcc agccctagga cgagccctgg ctcttcgcgg gtgccccggt
     4441 gcaaaacaga atagacgcac agcgcgcggt gcgagccgtc caaaatgccc ggcaactgag
     4501 cctcgaacgg gaagcccggc cgccacatga gcgagccgta gccgaagacc cagaattcag
     4561 acatgctcct cgtcagtcct cgtggccgtg cgggcggccg aaatccgcgt ccggcgtatc
     4621 ttggcctgcg tcgatgacgc cgcggcggat ggcgcgcgtg cgcgtgaaat gcttgaacag
     4681 ggcatcgccg tcgccgcgcc gtatcgcctt ggtgagtgct gcgacgtcct cgttgaaccg
     4741 gccgagcatt tcgagcaccg cgtccttgtt ggtcaggaat acgtcgcgcc acattgtagg
     4801 atccgaggcc gcgatgcggg tgaagtcacg gaagccaccg gcggagaatt tgagcacctc
     4861 cgagcgggtc accgtttcaa gctcgtccgc cgtgccgaca atcgtgtagg cgattagatg
     4921 cggcagatgg ctggtgaccg cgagcaccag gtcgtggtga tcgggcgtca tgatctcgac
     4981 attggcgccg aacagcgccc agaaggccgc caaacggtcc actgcttgcg ggtccgtgcc
     5041 ttcgggcggc gtcagaatgc accagcgtcc gtcgaacagt ttggcgaagc cggcgtcggg
     5101 gccggagtgc tcggtgcctg cgaccgggtg cgccggtacg aaatgcacat tgtccggcag
     5161 atgcgggctc atatcgcgca cgatcgcgct cttcaccgag ccgacatcgg agacgatggc
     5221 gccctcttta aggtgcggcg cgatttcttc ggccacggca ccgcaaatgc cgacgggtgt
     5281 gcacacgatc accaggtcgg cgttctcgac cgcgctcgcg ttgctgtcgg cgactttgtc
     5341 ggcaatgcca agctcggcga cgcgcttgcg tgtttcaggc gaccgcgaac aggttacgat
     5401 cgtgcgcacg gcgtcatgcg cacgcgcggc gcgcatgatc gaggagccga tcagcccggg
     5461 gccgataatg gccagcgtat cgaatagcgg tttcgtcatg cggcctcggt acccatgaat
     5521 tcggtcagcg cgtcgagcac ggccatgttt tcctctttgg tgccgatggt catgcgcagc
     5581 gcattcggaa agccatactc ctccacgcgc cgcaacacga tgcggcgtgc atgcaggaat
     5641 ttgtcggcat cgcgcgccga cttgccgggc gcgtccggaa aatgcaccag caaaaagttc
     5701 gccacactcg gcgtaacgcg caagccgatg cccgaaagcg cttcggtcat gacaatgcgc
     5761 cagtgcgtat tgtgcgccac ggccaattcc atatgcgcca cgtcgttcag tgcagcgaca
     5821 ccaacagcaa tagacgggcc cgtcacgttg aacggcccgc ggatacggtt cagcgcatcg
     5881 gcgatcggag ctgggcaata ggcccagccc agccgcaaag ccgcgagccc gtagatcttt
     5941 gaaaaggtgc gcgtcataac cgcattcggg ctcgtcgcca cgagctcgat cccggcctcg
     6001 tagtcgttgg cacgcacata ctccgcatag gccgcgtcga ggaccaagat gacgcgctcg
     6061 gggagccccg catgcaggcg cttgatctcg tcgaacggca cataggtccc tgtcgggtta
     6121 ttgggattgg cgatgaacac catgcgcgtc ttctcggtca cgcgtgcgag gatggcatcc
     6181 acgtttgccg tgagatcggt ctcggccgcg accacaggtg tggccccgcg cgcttgaatg
     6241 gcgatcctgt agaccaggaa gccgtgctct gtgtagatcg cctcatcgcc cggcccgaga
     6301 taggcgcaag cgagcaggtt cagaagctca tcggacccgg ccccacagac aatccgcgcg
     6361 gcatcgagcc cgtagcgggc ggccagcgcc tcacgcaagg gggctgccga cccgtcaggg
     6421 tagcgcgaca ggcagctggc ctcggccgaa taggcctcga cggcctttgg agagggtcca
     6481 agcggggtct cgttggagga gagcttgacc accgtgccgg cgccagggag cgttctcgtg
     6541 cccgggacgt agggcgcgat gtcgagaatg cctgggcgcg gggccggccc gcctttggcg
     6601 gccaacgatt tgggcgccat ggctctgctc cttcgagcgg ttgtggtcag cgaagggcct
     6661 ctcatacggc cattcttgag gccaaggcaa agaaaagatt gactttcatt gatgaggcca
     6721 tagctaaccc gaaattgtat aattcccggc caaatggcgt gtggcgcgcc tggataggcc
     6781 ttggcgcgca tgagtaggca gttttggcga tgaatgagac cccgggagcg gagcgcttcg
     6841 ccgacctcaa gcggcgggag cagaccacca atccccaaag cgagatcgtg cattttccgg
     6901 ccagcgagcc tttgccgctg gactgcgggg gtgcgctcgc gccgctgtcc attgcctacc
     6961 agacctacgg cacgctcaat gccgagaagt cgaacctcat cctcatctgc catgccctga
     7021 ccggcgatca gcatgctgcc aacctacacc ccatcaccgg caagcccggc tggtgggaca
     7081 ccatggtggg gccgggcaag tccatcgata cggaccgcta tttcgtgctg tgccccaacg
     7141 tcatcggcgg ctgtatgggt accacggggc cgagctccat caacccggag accgggcgcg
     7201 cttacgggcc cgactttccc gtcatcaccg tgcgcgacat ggtgcgggcg caagcgcgtc
     7261 tgctggatca tctcggcatc gatcaggtct tgagcgtgat cggcggctca atgggcggta
     7321 tgcaagtgct ggaatgggcc gcgacctatc aggaccgcgt gttctcagcg atcccgatcg
     7381 cctgcgcggc gcgtcattca tcgcagaaca tcgccttcca cgaggtcggg cgccaagccg
     7441 tcatggccga tcccgagtgg tgcgagggcc gctacctgga cgaaggcaag agcccccgca
     7501 aagggctcgc cgtggcgcgc atggccgcgc acatcaccta tctgtcggac ctcgcgctgc
     7561 acgacaagtt cggccgcaat ttgcaggacc gggagaaggt gacgttcggc tttgacgccg
     7621 acttccaggt ggagagctat ctgcgccatc aaggtatgag cttcgtcgac cggttcgacg
     7681 cgaactccta cctctacatc acgcgcgcga tggactattt cgacatcgcc gcggaccatg
     7741 acggtgtgct cgccaacgcg tttgccggca cgaagacgcg tttttgcatc gtgtcgttca
     7801 cctccgattg gctgttccca accgaggaga gccgcgatgt ggtgcatgcg ttgaacgcgg
     7861 tcgccgccaa tgtgagtttc gtggagatcg
//
LOCUS       DBNHVU010000062         4004 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_468965, whole genome shotgun sequence.
ACCESSION   DBNHVU010000062 DBNHVU010000000
VERSION     DBNHVU010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4004)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4004)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4004
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_468965"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..582)
                     /locus_tag="AC16Y7_02415"
     CDS             complement(1..582)
                     /locus_tag="AC16Y7_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5708264.1"
                     /translation="MASTLHHRAYRRDIEQLAVAVVDNRRPMRAMMRAMLAAIGVGRV
                     EIYESPANALSAMPQTAPDVVIAAGAMQPFGGVELVRFMHRPDSEPLCFVPAMIMSAH
                     AKPDLVAEALQAGAHQVLLLPTSAGTLFRRLDWLMNDERPFELIDDHYVVSGLEEQLA
                     LSTPRPTCAPGAVMAAASAVPAAAPHAVKRRAG"
     gene            968..>4004
                     /locus_tag="AC16Y7_02420"
     CDS             968..>4004
                     /locus_tag="AC16Y7_02420"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HIR5708265.1"
                     /translation="MRIQRRFTEPGQSPYAGIAFRESRSEIKNPDGSVVFALERFSVP
                     DKWSQVAADILAQKYFRKAGVPAKLKRVEENSVPSWLWRSEPDTEALQALPEDDRFGS
                     ETDARQVFDRLAGTWTYWGWKAGYFSSEDDASAFYDEMRYMLAMQMGAPNSPQWFNTG
                     LHWAYGIDGPSQGHYYVDFATGQLTASDSAYEHPQPHACFIQSIEDDLVNENGIMDLW
                     VREARLFKYGSGTGSNFSRLRGEMEPLSGGGKSSGLMSFLRIGDRAAGAIKSGGTTRR
                     AAKMVVVDIDHPDIEDYINWKVVEEQKVAALVTGSKVLSERLNAILRACANCDGDGDD
                     CFDPTLNPALKREVKAARKAKVPDNMVRRMIQFARQGYTSVDFPTYDTDWDSEAYRTV
                     SGQNSNNSVRVTDEFLNAVETDGDWDLIFRKNGQVGKRLKARELWEQVGYAAWSCADP
                     GIQFHTTINDWHTCPAAGDIRASNPCSEYMFLDDTACNLASLNLMGFRAEDGSFDVEG
                     YEHAIRLWTIVLEIAVTMAQFPSRQIAQLSYEYRTLGLGYANIGGLLMASGIPYDSDE
                     GRALGGALTAIMTGVSYATSAEMARELGPFPGYEDNREQMLKVIRNHARASRGERDGY
                     EALSTPPVPLDHAACPDPRLLAHAMRAWDQALELGRDYGYRNAQATVIAPTGTIGLVM
                     DCDTTGIEPDFALVKFKKLAGGGYFKIINRMVPEALSGLGYGEDDIKEIIDYAVGRGT
                     LAGAPGVNHETLAEKGFSPEKIKTIEEAVASAFDIKFVFNKWTLGEAFCTEDLGLAAD
                     RLDQADFDLLAEIGFSKADIDAANEYACGAMTLEGAPGLNAEHLPVFDCASPCGRKGT
                     RFLSVESHIRMMAAAQPFISGAISKTINMPTDASVEDCKEAYLLSWRLALKANALYRD
                     GSKLSQPLNAQLLADDEDEQDELLTAVASDQPAVQRAAVVTERIVERIVERAREAADR
                     ERLPHRRKGYTQKAVVGGHKVYLRTGEYADGRLGEIFIDM"
BASE COUNT          763 a         1282 c         1267 g          692 t
ORIGIN      
        1 ttagcccgcc cggcgcttga ccgcatgggg cgccgcagcc ggaaccgcgg aagccgccgc
       61 catcacggcg cccggtgcgc aagtaggccg cggggtgctg agggccagct gttcttcgag
      121 gcccgaaacc acatagtgat catctatcag ctcgaagggc cgctcgtcgt tcatcagcca
      181 gtccaggcgc cgaaacagtg tgccggcgga ggtcgggagc agaagcactt ggtgagcgcc
      241 cgcctgaagc gcctcggcca ccaggtccgg cttggcgtgt gcgctcataa tcatggccgg
      301 cacgaagcat aagggttcgg aatcgggccg gtgcatgaac ctaaccagct cgacgccgcc
      361 gaagggctgc atggcgccgg ccgcgatcac gacgtccggc gcggtctggg gcatggcgga
      421 gagggcgttg gctgggcttt cgtagatctc gacccggccc acgccgatgg cggcgagcat
      481 cgcgcgcatc atggcgcgca tgggccggcg attgtcgacc acggctacgg ccaattgctc
      541 gatgtcgcgc cgataagcgc ggtgatgcag cgttgaagcc aatttagatc ctcacggaag
      601 ccgttaccgt gccgaaccta ctgattatct tgtaaatttc ccatgaacgg gggatgcgga
      661 tctcagcccc cctccacccc gtccgtcgcc tctatcgcgg cccttgcgac gcgatcggac
      721 gcggaaaatc gccacaggcc tgtcgctcac caaaaaaatg gccgatttcc aagggtttgg
      781 ctcgccgcca ttgcaggtca tccagcgcct atccacatac tattcctatg ctcgcccaca
      841 gggtttagta tgcagtaagg actccctgcc atacatcttg ttgccggctc gcgagaatcg
      901 gagcagtttt tgaggttcga agaacgaacg atctaggggc cgaaatttac tagggggaag
      961 agctaatatg cggattcaaa gacgttttac tgagccagga cagtcgcctt atgccggaat
     1021 cgcgtttcgg gagagccgga gcgagatcaa gaatccggat ggctcggtcg tctttgcctt
     1081 agagcgcttc tccgtccccg acaaatggag ccaggtggcg gccgacatcc tggcccagaa
     1141 gtatttccgc aaagccggcg tgccggccaa gctgaagcgc gtggaggaga attccgtccc
     1201 ctcttggctg tggcggtccg aacccgacac cgaggcgctc caggccctac cggaagacga
     1261 ccgcttcggc agcgagaccg acgcccggca ggtcttcgac cggctggcgg gcacctggac
     1321 ctattggggc tggaaggccg gctatttcag ctccgaggac gatgcctccg ccttctatga
     1381 cgagatgcgc tacatgctcg ccatgcagat gggcgcgccc aacagcccgc aatggttcaa
     1441 taccggtctg cattgggcct atggcatcga tggcccgagc caggggcatt attatgtgga
     1501 cttcgccacc ggccagctga cggcctccga ttccgcctac gagcaccccc agccccacgc
     1561 ctgcttcatc cagtcgatcg aggacgatct cgtcaacgag aacggcatta tggatctatg
     1621 ggtgcgcgag gcgcgcctct tcaaatacgg ctccggtacg ggctccaact tctcccggct
     1681 gcgcggcgag atggagccac tttcgggcgg cggcaagtcc tccggcctga tgagcttctt
     1741 gcggatcggc gaccgggctg ccggcgccat caagtcgggc ggcaccacgc gccgcgcggc
     1801 caagatggtc gtggtggaca tcgatcatcc ggacatcgag gactacatca attggaaggt
     1861 cgtcgaggag cagaaggtgg ccgcgctcgt caccggctcc aaggtgctgt ccgagcggct
     1921 caacgccatc ctgcgggctt gcgccaattg cgacggcgac ggcgacgact gtttcgatcc
     1981 cacgctgaac ccggcgctga agcgcgaggt caaggcggcg cggaaggcca aggtgccgga
     2041 caacatggtc cggcgcatga tccagttcgc gcgccaaggc tacacctcgg tcgactttcc
     2101 gacatacgac acggattggg actcggaagc ctatcggacg gtctcggggc agaattccaa
     2161 caactcggta cgcgtgacgg acgagtttct gaacgcggtc gagacggacg gcgactggga
     2221 tctcatcttc cgcaagaacg gccaggtcgg caaaaggctg aaggcccgcg agctgtggga
     2281 gcaggtcggc tacgccgctt ggtcgtgcgc cgatccgggc atccagttcc acaccacgat
     2341 caacgattgg cacacctgcc cggcagcggg cgatatccgg gcctcgaacc cctgctcgga
     2401 atacatgttc ctggacgata cggcctgcaa tctcgcctcc ctcaatttga tggggttccg
     2461 cgcagaggac ggcagcttcg acgtcgaggg ctatgagcac gccatccggc tctggacgat
     2521 cgtgctggag atagccgtga caatggcgca gttcccctcg cgccagatcg cgcagctctc
     2581 ttatgaatac cgcacgcttg ggctcggcta cgccaatatt ggcggcctgt tgatggcctc
     2641 cggcattcct tacgactccg acgaaggccg ggcgctgggc ggtgcgctga ccgcgatcat
     2701 gaccggcgtc tcttatgcca ccagcgcgga aatggcccgc gaactcggtc ccttccccgg
     2761 ctacgaagat aaccgcgagc agatgctgaa ggtcatccgc aatcatgcgc gggcctcgcg
     2821 tggcgaaagg gacggctatg aagccctctc gacccccccg gtgccgctcg accatgcggc
     2881 ctgccccgac ccgcggctct tggcccacgc catgcgcgcc tgggaccagg cgctcgagct
     2941 cggccgcgac tacggctatc gcaacgcgca agccacggtg atcgcgccga ccggcacgat
     3001 cggcctggtg atggactgcg acaccaccgg catcgagccg gacttcgcct tggtgaagtt
     3061 caagaagctc gccggcggcg gctacttcaa gatcatcaac cgcatggtgc cggaggcctt
     3121 gagcggcctc ggctacggcg aagacgatat caaggagatc atcgactatg cggtcgggcg
     3181 cggcacgctt gccggcgcgc cgggcgtcaa tcacgagacg cttgccgaaa agggcttctc
     3241 gccggagaag atcaaaacga tcgaagaggc ggtcgcctcg gccttcgaca tcaagttcgt
     3301 cttcaacaaa tggacccttg gcgaggcgtt ctgcaccgag gacctcggcc ttgccgcgga
     3361 ccggctggac caagccgatt tcgatcttct ggcggaaatc ggcttctcga aggccgatat
     3421 cgatgccgcc aacgagtatg cctgcggggc catgacgctc gaaggcgcgc cggggctcaa
     3481 cgcggagcac ttgccggtgt tcgactgcgc gagcccctgc ggccgcaagg gcacccgctt
     3541 cttgtcggtg gaaagccaca tccgcatgat ggcggcggca cagcccttca tctcgggcgc
     3601 catctccaag accatcaaca tgccgaccga tgcgagcgtc gaggactgca aggaggctta
     3661 tctcctgtcc tggcgcttgg ccctgaaggc gaacgcgctc tatcgcgacg gctccaagct
     3721 gtcccaaccg ctcaacgcgc aactcctggc cgacgacgaa gacgagcaag acgagctgct
     3781 gacggcggtc gcctccgatc agccggccgt gcagcgcgcc gccgtggtca ccgaacgcat
     3841 cgtggagcgg atcgtcgaac gggcccgcga ggccgcagat cgggagcgcc tgccccaccg
     3901 gcgcaaggga tatacccaaa aagccgtggt cggcggtcat aaggtctatt taagaactgg
     3961 cgagtatgcc gatggtcggt tgggcgagat ctttatcgat atgc
//
LOCUS       DBNHVU010000063         2557 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_469137, whole genome shotgun sequence.
ACCESSION   DBNHVU010000063 DBNHVU010000000
VERSION     DBNHVU010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_469137"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..128)
                     /locus_tag="AC16Y7_02425"
     CDS             complement(<1..128)
                     /locus_tag="AC16Y7_02425"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017264273.1"
                     /note="catalyzes the transamination of D-amino acids and
                     their alpha-keto acids; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HIR5708266.1"
                     /translation="MPRIVYVNGVYLPQEEAKVSVFDRAFLFGDAVYEVTAVLDGRL"
     gene            299..682
                     /locus_tag="AC16Y7_02430"
     CDS             299..682
                     /locus_tag="AC16Y7_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708267.1"
                     /translation="MTSARLLLVLIVGTVVGAITGLVVGGGFNADYLAILAGFLGCLS
                     GVIVRNLIGLGPDDTKTPWLVAIYAIIASLAGSLAAKEVSDFSEITAPVGIGALAGLF
                     AALLLAMLLITYHTHPGHSPTLKKG"
     gene            complement(693..1712)
                     /gene="bioB"
                     /locus_tag="AC16Y7_02435"
     CDS             complement(693..1712)
                     /gene="bioB"
                     /locus_tag="AC16Y7_02435"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012456190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HIR5708268.1"
                     /translation="MGDDGLEAKAPARPHSAGAHQPETRNDWQTAEIVYLLKAPLLDL
                     LDEARAVHQQHHADGQVQLASLLSIKTGACPEDCKYCPQSAHYTKKTGLEREALLDVD
                     DVLAKAQIAKDAGASRFCMGAAWREVKDGPAFDSVLDMVRGVRALDMEACVTLGMLSE
                     AQAQRLAEAGLTAYNHNLDTSPEFYKEIITTRTYEDRLDTLANVRGAGMQVCCGGIIG
                     MGETIEDRARLLQELATLDPHPESVPINALVPVPGTPLEEQPPVEPLDLVRMVATARI
                     LMPASLVRLSAGRAALTREAQILCFLAGANSIFYGDQLLTTANNDVDDDLALIEEAGL
                     RIKPA"
     gene            1815..>2557
                     /locus_tag="AC16Y7_02440"
     CDS             1815..>2557
                     /locus_tag="AC16Y7_02440"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5708269.1"
                     /translation="MVGNETRYRAFLETLGQDRLKRTLTPVAARDARTLTVGGRAYVN
                     FASNDYLALRFHKVLIARAQDWAEAHGTGSGASRLVTGHLDLFEALEDKVARLKDKPA
                     ALLMASGFQANAAVLQALFDRTVLGAEPLVFADRLNHASMHFGCKAGGVRQLRYRHGD
                     ASHLAELLTPYQGDSRPKFILTESVFSMDGDIAPLAEISALAKTHDAMLIVDDAHATG
                     ILGEGGAGLSGGADLVVGTFSKALGSFGA"
BASE COUNT          427 a          813 c          818 g          499 t
ORIGIN      
        1 aggcgcccat cgagaaccgc ggtgacctcg taaaccgcgt cgccgaatag aaaggcgcga
       61 tcgaagacgg agaccttggc ctcctcctgg ggcagataga cgccgttcac atagacgatg
      121 cggggcatga agcgggagaa actcgaaagg cttgttcggc tctggttagc gccgggcttg
      181 gctggaggca agtcgtccgc cttgcagcgg gcatttcatg ctatatcgtc gggcgttctg
      241 ccgcgagggt agtcgaatcg cgccatttta gagcgcacct tttggggagg ggatgaggat
      301 gacttctgcg aggctattgc tggttcttat cgtgggaacc gtcgtcggtg cgatcaccgg
      361 cctggtcgta ggcggtggct tcaatgccga ctatctcgcc atcttggccg gatttctcgg
      421 ctgtctttcc ggcgtgattg tacgcaatct aatcggtctt gggcccgacg acacgaagac
      481 cccgtggctc gtcgcaatct acgcgattat tgcctcgctc gccggcagcc tagctgccaa
      541 ggaagtgtcc gacttcagcg agatcacggc accggtcggc atcggcgctc tggcaggtct
      601 cttcgcagca ttgctccttg cgatgctgct gatcacctac cacactcatc cgggccattc
      661 gccaaccctc aagaagggct gatcacgagg ctttaggccg gcttgatccg caggccggcc
      721 tcttcgatca gggcgagatc gtcgtccacg tcgttgttag ccgtggtcag cagctggtcg
      781 ccatagaaga tggaattggc gcccgcgagg aagcagagga tctgcgcctc gcgggtgagc
      841 gccgcgcgcc ccgccgacag ccgcacgagc gatgccggca tgagaatgcg cgccgtcgct
      901 accatgcgca ccaggtccag cggttcgacc ggcggctgct cttccagcgg cgtgcccgga
      961 accggcacca gcgcgttgat cggcacgctt tcggggtgag gatcgagcgt cgcgagctcc
     1021 tggaggagcc gcgcccggtc ctcgatagtc tcgcccatgc cgataatgcc gccgcagcac
     1081 acctgcatcc ctgcgccgcg cacatttgcc agcgtgtcga gccggtcctc ataggtccgc
     1141 gtggtgatga tctctttgta aaactccggc gacgtatcga gattgtggtt gtaggcggtg
     1201 agccccgcct cggcgaggcg ctgtgcttgg gcctcgctca acatgccgag cgtgacgcag
     1261 gcctccatgt cgagcgcacg cacgccgcgc accatatcca gcacgctatc gaaggccggc
     1321 ccgtccttca cctcgcgcca ggccgcgccc atgcaaaagc ggctggcgcc ggcgtccttg
     1381 gcgatctgcg ccttggccag cacgtcgtcc acgtccagca gtgcctcgcg ctcaaggccg
     1441 gtcttcttgg tgtagtgggc gctttgaggg cagtacttgc agtcctccgg gcaggcgccc
     1501 gtcttgatgg acaggaggct cgccagctgg acctggccgt ccgcatggtg ctgctggtgg
     1561 acagcacgcg cctcgtccag gaggtccagg aggggcgcct tcagcagata cacgatttcg
     1621 gcggtttgcc agtcgttccg ggtctctggc tgatgggctc cggccgaatg gggccgggct
     1681 ggagctttgg cctcaaggcc atcgtcgccc aagcgggcca gatcgttgct aggattcaag
     1741 ctttccaagg tctcagacaa gggacttcca agcgattgat tgctggggat aactcccttc
     1801 gctaatacag gatcatggtt gggaacgaaa cccggtaccg ggcctttctg gagaccctgg
     1861 gacaagaccg cctcaagcgg accttgaccc cggtggctgc gcgcgatgcg cggaccctga
     1921 ccgtgggcgg ccgagcctat gtgaactttg ccagcaacga ctatctcgcc ttgcgcttcc
     1981 ataaggtgct gattgcccgg gcccaagact gggcggaggc tcacggaacc ggctccggcg
     2041 cctcgcgcct tgtcaccggc catcttgacc tgttcgaggc gctcgaggac aaggtcgccc
     2101 gcctcaagga caagcccgct gcgctgctca tggcctccgg cttccaagcg aacgccgcag
     2161 tgttgcaggc gctgttcgat agaaccgtgc tcggcgccga gccgctcgtc tttgcggacc
     2221 gtttgaatca cgccagcatg catttcggct gcaaggccgg gggtgtccgg cagttacgat
     2281 accgccatgg cgatgccagc catctcgccg aacttctgac tccctatcaa ggcgatagcc
     2341 gtccgaaatt catcctcacc gagagcgtgt tctccatgga cggcgacatc gcgccactcg
     2401 cggagatttc ggcgctggcc aagacgcatg acgccatgct gatcgtggac gatgcgcacg
     2461 cgaccggcat cttgggcgag ggcggggcag ggctctccgg cggcgccgat ctcgtcgttg
     2521 gcacattctc caaggcgctc ggcagcttcg gtgccta
//
LOCUS       DBNHVU010000064         5226 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1807482, whole genome shotgun sequence.
ACCESSION   DBNHVU010000064 DBNHVU010000000
VERSION     DBNHVU010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1807482"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1108)
                     /locus_tag="AC16Y7_02445"
     CDS             complement(68..1108)
                     /locus_tag="AC16Y7_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050526 - poly(3-hydroxybutyrate)
                     depolymerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular catalytic domain type 2
                     short-chain-length polyhydroxyalkanoate depolymerase"
                     /protein_id="HIR5708270.1"
                     /translation="MRLHGLGVAIGLLAASAAVPAAAGATLPKLGTELPATSVSGLSS
                     GAYMAGQIEIAHSKDIVGAGIVAGGPFACAESPLASAFPYWPTAVAQNGTQALLRCMK
                     TTMGTPDPDRLVTRAKELAEKGEIDPLSALARHNVYLYSGDNDATVTRPVVKAAKRFY
                     DLAGVPASNVTFVTGDGGHAFITEEGGAACGVSEPPYVSDCDYDQARAILGWIYGPLE
                     PSSDAAQGAFVVFDQENFSDAGDGFAGEGVVYVPASCRTAPGCRVHVSLHGCGQSREK
                     IGDTFIRDSGFARIADTNRIVMLFPQISASAYNPEGCWDWWGYTGLDFLGKDAAQINA
                     IWSMVEHLAKQE"
     gene            1287..1826
                     /gene="speD"
                     /locus_tag="AC16Y7_02450"
     CDS             1287..1826
                     /gene="speD"
                     /locus_tag="AC16Y7_02450"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009759488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HIR5708271.1"
                     /translation="MSSDALFQLGMDLDHGSSTAQTEEEHSMCFDPQSGDERKDFFVE
                     RDGIRYAGTHLIIDLVRAERLDDLDHIDQTLRRCVDAAGATLLHIHLHRFTPNGGISG
                     VAVLAESHISIHSWPEHGYAALDVFMCGDTDPNATIDVMKEAFSPGKVVVKTELRGME
                     IQRWNDGSRRHSTTASASA"
     gene            1775..2629
                     /gene="speE"
                     /locus_tag="AC16Y7_02455"
     CDS             1775..2629
                     /gene="speE"
                     /locus_tag="AC16Y7_02455"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HIR5708272.1"
                     /translation="MERWVEETLHDGFRVRLKADKVLFDSKTEHQQLIIFENEEFGRV
                     MMLDGVVQFSTKDEFVYHEMMAHVPLFAHGKAKKVLIVGGGDGGVLREALRHESVESA
                     TLCEIDRSVIDLCREHFPDVSAGAYDDPRTNVVIDDGTKFVAETEKRFEVILVDSTDP
                     IGPGAVLFTKAFYAGCKRALEPGGILVTQNGLPFLQGPELKESAGYFRELFTDAFAYV
                     ATTPSYFGGSMSYGWASDDTTLRHHPLEEIERRYEAAGKFPTRYWWPEMQAAAFALPA
                     YIRELVEA"
     gene            complement(2642..3895)
                     /locus_tag="AC16Y7_02460"
     CDS             complement(2642..3895)
                     /locus_tag="AC16Y7_02460"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HIR5708273.1"
                     /translation="MVDLPAGNAVIGQSGGPTAVINQSLAGVVEGLQAGLAASGKVRQ
                     IFGMRHGVRGIAKGRTKGLVDLTSLTAEQLEAVARTPSAALGSTRDKPDDIYCAQILE
                     GCRDHDIRYFFYIGGNDSADTCRIVSEKAAEADYEMRCFHVPKTIDNDLRENDHTPGF
                     PSAARFVTLAHMGDALDNASLGGIKINIVMGRNAGFLTASAALARGLRETDLDPASRP
                     APHLIYLPEVPFIMDEFLADVDAVYTKLGRCQIAVSEGIRDGDGNEIGPQLMGTGEVD
                     AHGNVQLSGSGALGDGLAGVIKNGLTPEGGKPPRVRADTFGYLQRCWTDPSAVDAAEA
                     RGVGRHAAALAAEGDAGASITIVRTSNAVDSYRSAFGRAELSAVARHARHMPDAFVLG
                     RNDVSAAFYDYCLPLVGDLPDFTRL"
     gene            complement(3972..4967)
                     /gene="egtD"
                     /locus_tag="AC16Y7_02465"
     CDS             complement(3972..4967)
                     /gene="egtD"
                     /locus_tag="AC16Y7_02465"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052706 - L-histidine
                     N(alpha)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HIR5708274.1"
                     /translation="MSSPQVLSAALPADAPVAEPARRATEIAEFGEAVLSGLARHPRS
                     IPSRFLYDAVGSALFEYITRLQEYYPTRTETALLEAHGAGIAALSGDVRVLVEFGSGS
                     SRKTGLLIDALTNLDTYVPIDVSEAFLVEAAKRLMAQHDGLTVRPLVGDFTRPHTLDG
                     TPHGDRLGFFSGSTIGNLTHEEAHGFLENAAQLLGPDSAFLVGVDLKKSLDILIPAYD
                     DDGGVTASFSLNLLTRINRDLDGDFDTTRFAHVALYNADKGRIEIYLESLTDQDVQVL
                     GQTFSFTKGERIHTENSHKYSVPEFQTLAEDAGWTPVKVWTDEAQLFSLHLMRRA"
     gene            complement(4979..>5226)
                     /locus_tag="AC16Y7_02470"
     CDS             complement(4979..>5226)
                     /locus_tag="AC16Y7_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HIR5708275.1"
                     /translation="GLTQMFGDVWEWTSSAYLPYPGFKAAPGAVGEYNGKFMCNQFAL
                     KGGSCATPPGHVRRTYRNFFYPHQRWQFTGLRLAADA"
BASE COUNT          970 a         1640 c         1667 g          949 t
ORIGIN      
        1 tcgtagaggt tggagcccag cggcgcctcg aagatcaggg cagacggttc ctcggacatc
       61 gccggcgtta ctcctgtttt gcgagatgtt cgaccatcga ccagatggcg ttgatctgtg
      121 ccgcgtcctt gccgagaaaa tcgagcccgg tatagcccca ccagtcccag cacccttccg
      181 ggttataggc gctcgcggag atctggggaa agagcatgac gatacggttc gtgtcggcaa
      241 ttctggcgaa gcctgagtcc ctaatgaagg tgtcgccgat cttctcgcgc gactgcccgc
      301 agccgtggag tgacacatgg acgcggcagc ccggcgccgt gcggcatgag gccggtacgt
      361 agacgacgcc ttctcctgcg aaaccgtcgc ccgcatcgga gaagttctcc tggtcgaaca
      421 cgacgaacgc gccttgcgcg gcgtcggagg acggttcgag cggtccgtag atccagccga
      481 ggattgcgcg cgcctggtcg tagtcgcaat cactgacata aggcggctcg gaaaccccac
      541 atgccgcgcc gccttcctcg gtgatgaagg catggccgcc atcgcctgtc acaaatgtca
      601 cattgctcgc gggcacaccg gccagatcgt agaagcgctt cgcagccttc acaacaggcc
      661 gcgtgacagt ggcgtcattg tccccggagt agagatagac gttgtggcgc gccagggccg
      721 agagcggatc gatctcgccc ttctcagcca gctccttggc ccgcgtcacc aggcggtccg
      781 ggtccggcgt gcccatggtc gtcttcatgc aacgcaggag cgcctgcgtt ccgttttggg
      841 caacggcagt cggccagtac ggaaaggcgc ttgcaagcgg cgactcggcg catgcgaaag
      901 gcccgcccgc cacgataccg gcgccgacga tgtctttgga atgagcaatc tcgatctgcc
      961 cggccatata agcacccgat gacagccccg ataccgaggt ggcgggcagt tccgtgccga
     1021 gcttgggcaa ggtagcgccg gcagccgccg ggaccgccgc cgatgcggcc agcaacccga
     1081 tcgctacccc cagcccgtgc agacgcatcg cccgcgctcc cttttgattc ggcattgaat
     1141 cctagagcct tctcacgtcg ctttgttgaa gcttggcacc gggcatgttg ctccagccgt
     1201 ccaaaaaacc cttgcatacc aacaatgcga ggtgcaaata gcgcctctta ttcgactgga
     1261 tctatctgct ggttggggag gacgccatgt ccagtgacgc cctcttccaa ttggggatgg
     1321 acttggatca tggttcaagc accgcccaaa cggaagaaga gcattccatg tgtttcgatc
     1381 cccaatcggg tgacgagcgc aaggattttt tcgttgagcg agacggcatc cgctacgcgg
     1441 gcacgcatct catcattgat ctggttcggg cagaacggct cgacgatcta gaccacatcg
     1501 atcaaacgct gcggcgatgc gtggatgcgg cgggcgcgac gctcctgcat atccaccttc
     1561 atcgtttcac gcccaatggc ggcatctcgg gcgttgcggt tctcgccgag tcgcacatct
     1621 cgatccatag ttggcccgaa catggctacg ccgcgctcga tgtcttcatg tgcggtgaca
     1681 ccgatccgaa cgccaccatc gatgtgatga aggaggcgtt ctcgcccggc aaagtggttg
     1741 tcaaaacgga actccggggc atggagattc agcgatggaa cgatgggtcg aggagacact
     1801 ccacgacggc ttccgcgtcc gcctgaaggc ggacaaggtt ctcttcgata gcaagaccga
     1861 gcaccagcag ctcatcatct tcgagaacga ggagttcggc cgggtgatga tgctggatgg
     1921 cgtcgtccaa ttctccacga aggacgagtt cgtctatcac gagatgatgg cgcacgtgcc
     1981 gctgttcgcc cacggcaagg cgaagaaggt gctcatcgtc ggcggcggcg atggcggcgt
     2041 cttgcgcgaa gctctgcggc acgaaagcgt ggagagcgca acgctatgcg agatcgaccg
     2101 cagcgtgatc gatctttgcc gcgagcactt tccggatgtc tccgcgggcg cctatgacga
     2161 tccgcgcaca aacgtagtca tcgacgacgg caccaaattc gtggccgaga cggagaagcg
     2221 cttcgaggtc atcttggtgg attccaccga tcccatcggg ccgggcgccg tactcttcac
     2281 caaagcgttc tacgccggct gcaagcgcgc gcttgagcct ggcggcatcc ttgtcacgca
     2341 gaacgggttg ccgttcttgc aagggcccga gctgaaagag tccgctggct atttccgcga
     2401 actcttcacg gacgccttcg cctatgtggc gaccaccccg agctatttcg gcggctccat
     2461 gtcttatggc tgggcaagtg acgacacgac gctgcgccat cacccgttgg aagagatcga
     2521 gcgccgctac gaggcggccg gcaaattccc cacgcgctat tggtggcctg aaatgcaagc
     2581 ggctgccttc gccctgcccg cctatatccg cgagttggtc gaggcgtagt ctcagcaggc
     2641 tctaaagacg cgtgaagtcc ggcaggtcgc ccaccagcgg caggcagtaa tcgtaaaacg
     2701 ctgcgctcac gtcattgcgg cctaacacga acgcgtccgg catatgccgt gcgtgccgcg
     2761 ccacggccga aagctccgcc cgcccgaaag cgctgcgata gctgtcgacc gcgttcgagg
     2821 tgcgcacgat ggtgattgat gcgcccgcat cgccctcagc ggcaagcgct gctgcatgcc
     2881 gccccacgcc gcgcgcctcg gcggcgtcca ctgcggaggg atccgtccag cagcgctgca
     2941 gataaccgaa cgtgtcggcg cgcacgcgcg gcggcttgcc gccttccggc gttaggccat
     3001 tcttgatgac gcccgcaagc ccgtcgccga gcgcgccgga gcccgagagc tgcacattgc
     3061 cgtgcgcatc cacctcgcct gtgcccatga gctgcggacc gatctcgttg ccatcaccgt
     3121 cgcgaatgcc ttcgctcacc gcgatctggc agcggccgag cttggtgtag accgcgtcca
     3181 cgtcggccag gaactcatcc atgatgaacg gcacctcggg aaggtagatg aggtgcggtg
     3241 caggccggct tgcggggtcg aggtccgtct cgcgcaagcc ccgcgccagt gcggcagacg
     3301 ccgtaaggaa gccggcattg cggcccatga cgatgttgat cttgatgccg ccaaggctcg
     3361 cattgtcgag ggcatcgccc atatgcgcga gcgtcacgaa gcgcgccgcg gacggaaagc
     3421 ccggcgtgtg gtcgttctcg cgcagatcgt tgtcgatcgt tttcggcacg tggaagcagc
     3481 gcatctcgta gtcggcctcc gccgccttct cgctgacgat gcggcacgta tcggcggaat
     3541 cgttgccgcc gatatagaag aagtagcgga tgtcgtggtc gcggcagccc tcgagaatct
     3601 gcgcgcagta gatatcgtcg ggcttatcgc gggtggagcc cagagccgcg gacggggtcc
     3661 gcgcgacggc ctcgagctgc tccgcggtga ggctggtcaa atcgaccagg cccttggttc
     3721 ggcccttggc gatgccgcgc acgccatggc gcatgccgaa tatctgccgc accttgcccg
     3781 atgcggcgag gccggcttgc aagccctcga caacaccagc cagcgattga ttgataacgg
     3841 ctgtcggacc accggattga ccgatgaccg cgttgccggc aggaagatcg accacgcccc
     3901 ctccttgagt gtttggaggc ggcaacatgc cctggtgtta tccgccgcgc aaggccggca
     3961 gaatgttgcg gctaagcgcg ccgcatcaga tgcaggctga acagttgagc ctcgtcggtc
     4021 cacactttca ctggggtcca gccggcgtct tcggccaggg tctggaactc cggcaccgaa
     4081 tatttgtgtg agttctccgt gtggatgcgc tcgcccttgg tgaaggagaa ggtctgcccg
     4141 aggacctgca catcttggtc ggtgaggctc tccagataga tctcgatccg gcccttgtcg
     4201 gcattgtaaa gcgcgacgtg ggcgaagcgc gtcgtgtcga agtcgccgtc gaggtcacgg
     4261 ttgatgcgcg tcagtaggtt caagctgaac gaggcggtca cgccgccgtc gtcgtcatag
     4321 gcggggatca gaatgtcgag actcttcttg aggtcgacgc caacgagaaa ggcgctgtcc
     4381 ggtcccagaa gctgcgcggc attctccaaa aagccatgcg cttcttcgtg ggtaaggttc
     4441 ccgatggtcg agccggagaa aaagccgagc ctgtcaccgt gtggcgtacc gtcaagcgtg
     4501 tgcggtcggg tgaaatcgcc caccaacggt ctcacggtca gcccgtcatg ctgggccatg
     4561 agccgcttcg cagcctcgac gagaaacgct tcggagacgt cgatggggac ataggtatcg
     4621 agattggtaa gagcgtcgat cagcagaccg gtcttgcggc tcgagccgga gccgaactcg
     4681 acgagaacgc gaacatcgcc cgaaagcgcc gcgatccccg cgccgtgcgc ttccagcagc
     4741 gctgtctccg tgcgcgtcgg gtagtactcc tggaggcgag taatgtactc gaacagcgcc
     4801 gagcccaccg cgtcgtagag gaagcgggag ggaatgctgc gcgggtgcct ggcgagcccg
     4861 ctcaacacag cctcgccaaa ctccgcgatc tccgtcgcgc ggcgcgcagg ttcggcgaca
     4921 ggagcgtcgg ccggcaacgc cgcggacaag acctgagggg acgacacgtc gctcgccact
     4981 atgcgtccgc cgcaaggcgc aggcctgtga actgccagcg ctggtgagga tagaagaaat
     5041 tgcgataggt ccgccgcacg tgcccgggcg gtgtcgcgca ggagccgccc ttgagtgcga
     5101 actggttgca catgaacttg ccgttgtatt caccgacggc gccgggggca gccttgaagc
     5161 ccgggtaagg caggtaggcg cttgacgtcc attcccacac atcgccgaac atctgggtta
     5221 gccccg
//
LOCUS       DBNHVU010000065         4469 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1214471, whole genome shotgun sequence.
ACCESSION   DBNHVU010000065 DBNHVU010000000
VERSION     DBNHVU010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1214471"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1505
                     /locus_tag="AC16Y7_02475"
     CDS             <1..1505
                     /locus_tag="AC16Y7_02475"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIR5708276.1"
                     /translation="AKLTPAAQDLDKKTKGLLTKAIQITTFKGAKGKTVDVLAPQGTK
                     FTRIVLVGLGKPASYGHEDWLNLGGTVRGLLTGREGTTANVYLETVKSDVAPADVAAF
                     SLGAALRGYSFKKYKSKPKKTRGAASDKSLKKLIVHCADPRAAGQAYTPSRAIVDGVN
                     MARDLVNEPANVLGPAEFAQRAKELTKAGVKVEVLSEAALKRHGMLSLLAVGQGSGRP
                     SHVVVMKWNGAGPKGGAPIAIVGKGVTFDTGGISLKPAGGMEDMKGDMAGAACVVGLM
                     LALAERKAKVNAVGVIGLVENMPSGIATRPGDVVTGMSGTTIEVLNTDAEGRMVLADC
                     LWYAQEKFKPKAVVNLATLTGAVMVALGKEHAGLFSNNDELAERLTEAGKETGEKLWR
                     LPLGPKYDKLIESKVADIKNTGGRWGGSISAAQFLQRFVKEGTPWAHLDVAGTAMASA
                     NSEINRSWGSGFGVRLLDQLIKDHYEHSASSRSTASKSKSRSKARRKKAS"
     gene            1676..2497
                     /gene="mazG"
                     /locus_tag="AC16Y7_02480"
     CDS             1676..2497
                     /gene="mazG"
                     /locus_tag="AC16Y7_02480"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HIR5708277.1"
                     /translation="MAADSPKTYDLDDLLAVMARLRDPDTGCPWDVKQSFATIAPYTI
                     EEAYEVADAIAREDFDGLKDELGDLLLQVVYHAEMASEQSRFAFADVVDAITSKMIRR
                     HPHVFEDPSRREEFMTSDLWDRIKAEEKAECGEDSTSQSVLSGVPAGMPGLTRAVKLQ
                     RRAAKVGFDWPDLGPVMAKVDEELGELRHAIAANAASQAVEEEFGDLLFVLANVARHL
                     GLDPEAALRGANRKFVRRFEHIEAALAEQGRRPRESSLEEMDDLWDAAKKAERKE"
     gene            complement(2507..3847)
                     /gene="hflX"
                     /locus_tag="AC16Y7_02485"
     CDS             complement(2507..3847)
                     /gene="hflX"
                     /locus_tag="AC16Y7_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR5708278.1"
                     /translation="MRRDERVGAVVFVPILANKTASAKRGRAGDAPERTPQARLDEAV
                     GLAAAIDLDVRASGVVTVAQPKPATLFGSGKVEELAGIVRAEDAALVIVDHPVTPVQQ
                     RNLETAWNAKVLDRTGLILEIFGERARTREGRLQVELAHLVYQKSRLVRSWTHLERQR
                     GGFGFLGGPGETQIETDRRLLSERIDVIRKELDQVRRTRALHRKSRQRVPYPAVALVG
                     YTNAGKSSLFNTLTGAEVLAQDILFATLDPTIRALTLPGGSKAVLSDTVGFISDLPTA
                     LVAAFRATLEEVQEADLVLHVRDIADEASASQRADVNAVLADLEIDAEAEPDRVLEVW
                     NKADLLDPVARERAQNEAPRYGASLVSAVSGFGLDALLAEIERRLNRRRQTVQINVQP
                     EEGALSNWIYENCEVIARSDLADGVTSLKIRVAPEKRDRLARVAGPSRLKAAAE"
     gene            complement(3914..4168)
                     /gene="hfq"
                     /locus_tag="AC16Y7_02490"
     CDS             complement(3914..4168)
                     /gene="hfq"
                     /locus_tag="AC16Y7_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012171604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HIR5708279.1"
                     /translation="MATERTQSLQDTFLNHVRKSKTPLTIFLVNGVKLQGVVTWFDNF
                     CVLLRRDGHSQLVYKHAISTIMPGQPIQLSDITDDEGASE"
     gene            complement(4337..>4469)
                     /locus_tag="AC16Y7_02495"
     CDS             complement(4337..>4469)
                     /locus_tag="AC16Y7_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5708280.1"
                     /translation="AQVNRFGGNISRTAEFVGMERSALHRKLRALGVNSNERRAAMR"
BASE COUNT          852 a         1319 c         1472 g          826 t
ORIGIN      
        1 gggccaagct gacgcccgca gcacaggatc tcgacaagaa gaccaaaggg ctcctgacca
       61 aggcgattca aatcaccacg ttcaagggcg ccaagggtaa gaccgtcgac gtgcttgcgc
      121 cgcaaggcac caagttcaca cgcattgtgt tggtgggttt gggcaagcct gcatcgtacg
      181 gccacgaaga ttggctcaat ctcggcggca ccgtgcgcgg tttgctgacc ggccgggaag
      241 ggaccacggc caacgtttat ctggagacgg tcaagagcga cgtcgcgcct gctgatgtgg
      301 cggccttttc gctgggggct gcactgcgcg gctacagctt caaaaagtac aagtccaagc
      361 cgaagaagac gcgcggcgcc gcgagcgaca agtcgctcaa gaagctcatc gtgcattgcg
      421 ccgatccgcg cgcggcgggg caggcctata cgccgagccg cgccattgtc gatggcgtca
      481 acatggcgcg cgatctcgtc aacgaaccgg cgaacgtgct tggccctgcg gaattcgcac
      541 agcgcgcaaa ggaactcacc aaggccggcg tgaaggtcga ggtcttgagc gaagcagcat
      601 tgaaacgcca cggcatgctg tcattgctcg cggtcgggca gggtagcggg cgaccgagcc
      661 atgtcgtcgt gatgaaatgg aacggcgcgg ggccaaaggg cggtgcgccc atcgccatcg
      721 tcggcaaggg tgtgaccttc gacacaggtg gcatctcgct gaagcccgcg ggcggtatgg
      781 aggacatgaa gggtgacatg gcaggcgcgg cctgcgtcgt cggcctcatg ctcgcgctcg
      841 ccgaacgcaa agcgaaggtc aacgccgtcg gtgttatcgg gctcgtcgag aacatgccca
      901 gtggcatcgc gacacggcct ggagacgttg tgaccggcat gtccggcacg acgatcgaag
      961 tgctgaacac cgacgccgaa gggcgcatgg tactcgccga ttgtctctgg tacgcgcagg
     1021 agaagttcaa accgaaggcg gtggtcaacc ttgcgacgct gaccggtgcc gtcatggtgg
     1081 cgctcggcaa ggagcacgca ggtctgttct ccaacaacga cgagttggcc gagcggctga
     1141 cggaagcggg caaggagacg ggcgagaagc tgtggcggct tccgctcgga ccgaaatacg
     1201 acaagctgat cgagtcgaag gtcgccgaca tcaagaacac cggcggacgg tggggcggtt
     1261 cgatctccgc cgcgcagttc ctgcagcgct tcgtcaaaga gggaacgccc tgggcgcatc
     1321 tcgacgtggc cggcacggcc atggcgtcgg cgaacagcga gatcaatcgg agttggggct
     1381 caggctttgg cgtgcggctg ctcgatcagc tgatcaagga tcactacgag cacagcgcct
     1441 cgtcgcgatc gactgcgtcg aagtccaagt cgaggtccaa ggcgagacgg aagaaggcgt
     1501 cgtagcgcgc cgtcatttcg gcgccgccac ctcaggaaca tcacataggg atattttcca
     1561 taatatacct tatgcgaatc ggggatgtgg ggcacggatt tggccagaat tctctattac
     1621 cctgattttg cagcgtattt taggtttttg cgcgcggacc ccttgaggcc tcgtcatggc
     1681 cgctgacagc ccgaaaacgt acgatctcga cgatctcctc gccgtcatgg cgcgtttgcg
     1741 cgatcccgac accggctgtc cgtgggacgt gaagcagagt ttcgcgacga tcgcccccta
     1801 caccatcgag gaagcttacg aggtcgccga cgccattgcg cgtgaggact tcgacggcct
     1861 caaggacgag ctcggcgatc tcctgttgca ggtcgtctat cacgcggaga tggcgtcgga
     1921 gcagtcgcgc ttcgcctttg ccgacgtcgt ggacgccatc acgagcaaaa tgatccggcg
     1981 gcatccgcac gtgttcgagg atccctcgcg gcgcgaagag ttcatgacga gcgatttgtg
     2041 ggaccgcatc aaggctgaag agaaagccga gtgcggcgag gattccacat cccagagcgt
     2101 cctcagcggc gtgcccgccg gcatgccggg tttgacacgc gccgtgaaac ttcagaggcg
     2161 cgcggcgaag gtcggcttcg actggccgga ccttggtccg gtgatggcca aggtcgacga
     2221 ggaactcggc gagctccggc acgcaatcgc cgcgaacgcg gcgtcgcagg ccgtcgagga
     2281 agaattcggc gacctgctgt tcgtgttggc gaatgttgcg cgccatctcg gacttgaccc
     2341 ggaagcggca ttgcgtggcg cgaatagaaa attcgtacgg cggttcgagc acatcgaggc
     2401 ggcgctggct gagcaaggcc gaagaccgag ggagtcctcg ctggaggaga tggatgactt
     2461 gtgggacgcg gcgaaaaagg ccgagcgtaa agagtaggtg cgccccctac tcggctgccg
     2521 ccttgagcct cgatgggccg gcgacgcgcg ccagcctatc gcgcttctcc ggtgcaactc
     2581 tgatcttcaa tgacgtgacg ccgtcggcaa gatcggaccg ggcgatgacc tcgcaattct
     2641 catagatcca gttcgacaac gcgccttctt ccggctggac gttgatctgc acggtttgac
     2701 gtcgacggtt gagacgacgt tcaatctcgg cgagaagcgc atcgagaccg aagccgctga
     2761 cggccgagac gagactggcg ccgtagcgcg gtgcctcgtt ttgcgcgcgc tcgcgggcaa
     2821 ccggatcgag aaggtccgcc ttgttccaga cctcaagtac ccggtcgggc tcggcctcgg
     2881 catcgatttc gagatcggcg aggaccgcgt tcacgtcggc gcgctggctg gcgctcgcct
     2941 cgtcggcgat atcgcgaaca tgcaggacca gatctgcctc ttgaacctct tcgagtgtgg
     3001 cgcgaaacgc ggcgacgagc gccgtcggca agtcggaaat gaagcccacc gtgtcggaga
     3061 gcacggcctt cgatccgccc ggcaaggtca gtgcgcggat cgtcggatcg agcgtggcaa
     3121 acagaatatc ttgcgcaaga acttcggcgc cggtcagcgt gttgaacagc gaggacttgc
     3181 cggcgttcgt atagccgacc agcgccacgg ccggatacgg cacacgctgg cgcgacttgc
     3241 ggtgtagcgc gcgcgtgcgg cgaacttgat cgagctcttt tcgaatgacg tcgatgcgtt
     3301 cgctgagcaa gcggcggtcg gtttcgattt gtgtctcgcc cgggccgccg agaaagccga
     3361 agccaccacg ttgacgttca aggtgagtcc aggagcggac caggcggctc ttttgataga
     3421 cgagatgggc cagctcgact tggaggcgcc cttcccgcgt gcgcgcccgc tcgccgaaaa
     3481 tctcaaggat cagcccggtg cgatcgagga ccttcgcgtt ccaggctgtt tcaagattgc
     3541 gctgttgaac gggcgttacc gggtgatcca cgatgacgag tgcggcgtcc tcggcgcgga
     3601 cgatgcctgc cagctcttcg accttgccgg atccaaacag agttgcaggt ttcggctggg
     3661 cgacggtcac gacgccgctc gcccgcacgt cgagatcgat ggcggctgca aggcccaccg
     3721 cctcgtccag gcgggcttgc ggcgtgcgct ccggcgcgtc accggccctg ccccgcttgg
     3781 cgctcgcggt cttattggcg agaatgggca cgaacacgac cgcgcccacg cgctcgtcgc
     3841 ggcgcatgtc ccgtgcgctg cgttcttcct tgcccttctt acgctttaac tgtcttgggt
     3901 cggtaggacg caattattca gacgcacctt cgtcgtcggt gatgtcggac agctggatcg
     3961 gctgaccggg catgatggtc gagatggcat gcttgtatac gagctgtgaa tggccgtctc
     4021 ggcgcaagag cacacagaaa ttgtcaaacc acgtcacgac gccttggagc tttacaccgt
     4081 tcaccaggaa gatggtcagc ggcgtcttgc tcttacgaac atggtttaag aacgtatctt
     4141 gtaggctttg cgtgcgttca gttgccatgg gcttccccgt ccctttggtc cacgcttacg
     4201 cttgttggat ggcgccggaa tcgctccaac cggccgccgg attgcattag acgcgatcgc
     4261 aaggtgtttc tgcaagggtt ctgattccca tgccctttgc agcagcccct aaaagcgaag
     4321 cgagtcggcg gaaatgctag cgcatggcag ctcttctttc attggaattg acgccgagag
     4381 cccgaagttt ccggtggagg gctgagcgtt ccatgccgac aaattcggcc gtgcgggaga
     4441 tgtttccgcc gaatcggttg acttgggcc
//
LOCUS       DBNHVU010000066         5758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1957564, whole genome shotgun sequence.
ACCESSION   DBNHVU010000066 DBNHVU010000000
VERSION     DBNHVU010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5758
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1957564"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="AC16Y7_02500"
     CDS             complement(<1..244)
                     /locus_tag="AC16Y7_02500"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215473.1"
                     /note="with component II, the glutamine amidotransferase,
                     catalyzes the formation of anthranilate from chorismate
                     and glutamine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIR5708281.1"
                     /translation="MSPAPVPVTPLPRDGGSAPVEPDVASFARLYDDGVPQVVWTRLV
                     ADLETPVSAMLKVAHEWPNSFLLESVEGGATRGRYSI"
     gene            complement(241..2142)
                     /locus_tag="AC16Y7_02505"
     CDS             complement(241..2142)
                     /locus_tag="AC16Y7_02505"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR5708282.1"
                     /translation="MALDTLRKGAARTLGLILVALLVVSFAVWGIADIFTGYGRQTLI
                     SVGDTDISSQDYARVQQDVLRSMSQQQGRSLSLQEARAAGLDTRVLERLIGGAAVDTH
                     AKHLGLGVSDDALLAQIMKDPAFQDASGNFSPLNFQAALRNIGMNEASYLYTLREQNL
                     RRQLLVTVGEVAQSPKALMDALNQFNEERRILKYVLVPKSAADGVGAPTDEDLKKFYD
                     NHQAKFTQPEYRKVGVLAVTPETVKDELNITEDDLKATYEAKKETLGNVERRQLQQIA
                     LPDMAAAKAAHQKITSGTDFVEVAKELGLTETDIDLGTVSKKDFADVAIAEAAFKLDK
                     DAVSEPIEGKLGSFAVVRVVEIEPEKIPTFDEAKADLEKEILKDRSSGAIFDVHDQVE
                     DELAAGSTLEEAAGKLKLDYKVIDQVDREGRTPGGSAVTLPAQKDLLIGVFATDAGIE
                     NDPIDARDEGVIWYEVLGVTPSQLQPFDEVREAVESDWRGEATRNQVSKFAQGLVSAL
                     NNGSKTLDGVAQELDTEILPTSALKRDDITVNVLPAAVSQAFTLPKGGFGSAPSGVDE
                     GRIVFQVEDIVAAPVLDERVKKRLNSQIGLLLSEDMIAEYFSALESRYGVRVNQQALA
                     KLVGTSEEP"
     gene            2315..3088
                     /gene="tpiA"
                     /locus_tag="AC16Y7_02510"
     CDS             2315..3088
                     /gene="tpiA"
                     /locus_tag="AC16Y7_02510"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR5708283.1"
                     /translation="MTAIRPLVAGNWKMNGTSSALGELVALRDRLEKAPLPEVEVMVC
                     PPATLLSRAASVLAETAIALGAQDCHPLPSGAHTGDISAEMLADAGATAVIVGHSERR
                     IDHGETSETVNAKARAAYRAGLTAIVCIGETEEQHRDGETVDVLTSQIQGSVPEDAQG
                     SNTVLAYEPVWAIGSGLTPTPEDVARLHGVIRETLGQVLGPDQGGATRILYGGSVKPS
                     NAAELMSIPNVNGALVGGASLKASDFWEILAVYRSDSAT"
     gene            3187..3753
                     /locus_tag="AC16Y7_02515"
     CDS             3187..3753
                     /locus_tag="AC16Y7_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708284.1"
                     /translation="MSKLRPIAIALLAAFTAAPMSAGAEPLEREECKALESQKRTMLT
                     PKIKTALKQGPDWVKKNLHDAAEIEKIREYLQVEEKVAFRCRTDGVRIPKPLPPPLPD
                     RKPAVPTYVADGAPKILAGVAATSFLPLRNPSRSDTKTVEADPPAGEESAGEGDITTA
                     ADEEAEAGPSQAIADSDKTAPSENKATQ"
     gene            3750..4118
                     /gene="secG"
                     /locus_tag="AC16Y7_02520"
     CDS             3750..4118
                     /gene="secG"
                     /locus_tag="AC16Y7_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR5708285.1"
                     /translation="MSTVILLIHVMIALAMVGVILLQRSEGGALGIGGGGGAGGGGFM
                     TGRGAGNALTKTTAILAACFFVTSLTLSILATQQRGDTPSILPSGSGGEGGLNPLQIP
                     GTPAPKTSDTPAAPQAPQSQ"
     gene            4209..>5758
                     /locus_tag="AC16Y7_02525"
     CDS             4209..>5758
                     /locus_tag="AC16Y7_02525"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR5708286.1"
                     /translation="MARYVFITGGVVSSLGKGLASAALGALLQARGFSVRLCKLDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGRPASQADNITTGRIYQEIIAKE
                     RRGDYLGSTVQVIPHVTDAIKAFVTTGNEDVDFVLCEIGGTVGDIEGLPFFEAIRQLG
                     NELPRGQSAYIHLTLLPFIPSAGELKTKPTQHSVKELRSIGIQPDVLLCRADRTIPPD
                     ERRKIALFCNVRPEAVVQALDVASIYDVPIAYHEEGLDRELLAAFGITDAPDPDLRMW
                     RQISETIAEPDGEVTVAIVGKYTGLQDAYKSLNEALVHGGIANKVKVNLEWIEAQIFE
                     SQDAAAYLENVNGILVPGGFGERGAEGKIKAARFARTKQLPYFGICFGMQMAVVEAAR
                     NLAGIEGASSTEFGPSAEPVIGLMTEWMKGDELERREQNGNLGGTMRLGAYDAVLARG
                     SKVAEIYGDTEISERHRHRYEVNMRYRNKLEAAGLRFSGLSPDGLLPEIVEIPEHPWF
                     VGVQFHPEL"
BASE COUNT         1099 a         1912 c         1726 g         1021 t
ORIGIN      
        1 taatcgagta gcggccgcgc gtggcgccgc cctcgacgga ctccaagagg aaactgttgg
       61 gccactcatg cgcaaccttg agcatcgccg aaaccggcgt ctccaggtcg gccacgagcc
      121 gcgtccagac gacctgcggc acgccgtcgt catacagacg cgcaaagctc gcgacgtccg
      181 gttcgaccgg ggccgaaccg ccgtcacgcg gaaggggtgt caccgggacc ggcgccgggc
      241 tcatggctcc tcgctggtgc ccaccagctt ggcgagagcc tgctgattga cgcgaacgcc
      301 ataccggctc tccaatgcgc tgaaatactc cgcgatcata tcctcgctga gcaagagccc
      361 gatctgggaa ttcagccgct tcttgacgcg ttcatcgagt accggcgctg cgacgatgtc
      421 ctcgacctga aacacgatgc ggccttcgtc gacgcccgag ggcgccgaac cgaacccgcc
      481 cttcggcaaa gtgaaggcct gggacaccgc ggcgggcagc acgttcacgg tgatgtcgtc
      541 ccgtttcaaa gccgaagtcg gcaggatctc cgtatcgagc tcttgagcga cgccgtccag
      601 cgtcttgctg ccgttgttca aagccgagac caagccttgc gcgaacttgg agacctggtt
      661 ccgcgttgcc tcaccccgcc agtcgctctc gactgcctca cgaacctcgt cgaacggctg
      721 caactgcgag ggcgtcacgc cgaggacctc gtaccagatg acaccttcgt cgcgcgcatc
      781 tatcggatcg ttctcgatcc cggcatcggt ggcgaacaca ccgatcagca gatccttctg
      841 cgccgggagc gtgaccgccg acccgcccgg tgtgcggccc tcgcgatcga cttgatcgat
      901 cactttgtaa tcgagcttca gcttgcccgc cgcctcttcg agcgtcgatc ccgcagcgag
      961 ctcgtcctca acctggtcat gcacgtcgaa gatggcgccc gaagaccggt ccttgagaat
     1021 ctctttctcc agatcggcct tcgcctcgtc gaatgtcgga atcttctccg gctcgatttc
     1081 gaccacccgc acgaccgcga agcttccaag cttgccctcg atgggctcgc tgacggcatc
     1141 cttgtccagc ttgaacgccg cctcggcgat cgccacatcg gcgaagtcct tcttcgaaac
     1201 agtgccaaga tcgatgtccg tctcagtcag acccagctcc ttggcgactt cgacgaaatc
     1261 ggtccctgag gtgatcttct gatgggccgc tttcgcggct gccatgtccg gcaaagcgat
     1321 ttgctggagc tgacggcgct ccacgttccc aagcgtctcc ttcttggcct cgtacgttgc
     1381 cttgagatcg tcttccgtga tgttgagctc gtccttgacc gtctcgggtg tcacggcgag
     1441 cacaccgacc ttgcggtatt ccggctgtgt gaacttcgct tggtgattgt cgtaaaactt
     1501 tttcaagtcc tcgtcggtcg gcgcgccaac gccgtccgcg gccgacttcg gcacaagcac
     1561 atatttcagg atacgccgtt cctcgttgaa ctggttcagc gcatccatca atgccttggg
     1621 gctctgcgcc acttcgccaa cggtgacgag cagctgccgc cgcaaattct gttcgcgcag
     1681 cgtgtaaaga tagctcgctt cgttcatgcc gatattgcgc aaggcggcct ggaagttgag
     1741 cgggctgaaa tttcccgacg catcctggaa ggccggatcc ttcatgatct gcgcgagcag
     1801 cgcatcgtcg ctgacgccga ggcccaggtg cttcgcatgg gtatcgacgg cagcgccgcc
     1861 gatgagccgc tccaggacac gcgtgtcgag cccggcggct cgcgcttcct gcagactgag
     1921 gctgcgtccc tgctgctgac tcatcgagcg cagcacatcc tgctgcacgc gggcatagtc
     1981 ctgcgagctg atatccgtat cgccaacact gatcagggtt tgccggccgt agccggtgaa
     2041 gatgtctgca ataccccaga cggcgaagct caccaccagc agggcaacca agatcagacc
     2101 caatgttcgt gcagcgcctt tgcgcaatgt gtcgagtgcc acgcctcgaa aaccttctct
     2161 gtgatcaatt gccaaatccg cgccggcgtc cgatgctaag acgctccagc gcggcgcgcg
     2221 catcataggt agccaatgcc agcgcggcaa cacgcagaaa tacctgaaca tccgcatttc
     2281 aagtgccaca gtgagcaggg accgaggagg ggacgtgact gcgatcagac cgcttgtcgc
     2341 cggcaattgg aagatgaacg gcaccagcag cgcgcttggc gagctagtcg cgttgcggga
     2401 tcggctggag aaggcgccgc tcccggaggt cgaagtcatg gtttgcccgc ctgcgaccct
     2461 cctgtcacgc gccgccagcg tcctcgccga gaccgcgatc gcgcttggcg cccaggattg
     2521 ccatccgctt ccctctggcg cccataccgg cgacatttcc gccgaaatgc tggccgacgc
     2581 gggcgccacg gccgttatcg tcggccattc cgagcggcgc atcgaccacg gcgagaccag
     2641 tgaaaccgtc aacgccaagg cacgcgctgc ctatcgcgca gggctgaccg cgatcgtctg
     2701 catcggcgag acagaggagc agcaccgcga cggcgagacc gtcgacgtcc tcacctctca
     2761 gatccaggga tcggtgccgg aagacgccca aggctccaac accgtgctcg cctacgagcc
     2821 tgtttgggcc atcggctctg gcctcacgcc cacgcctgag gacgtggccc ggctgcatgg
     2881 ggtcatccgg gagaccctgg gccaggtctt ggggccggac caaggcggcg ccacgcgaat
     2941 cctctatggc gggtcggtca agcccagcaa tgccgccgag ctgatgtcca tccccaacgt
     3001 caacggtgcc ctggtcggcg gcgccagcct aaaggcctca gatttctggg aaattctcgc
     3061 tgtctatcgg agcgactcgg ccacgtagcg ggtcccgaaa accttgggcc ccggcttgca
     3121 aatcagataa ggttcgcctt gccatccacc tgaggatggc gggggaacca cgatcccggg
     3181 aatgagatgt caaagctgcg cccgattgcc atcgcgctgc ttgccgcttt taccgccgcg
     3241 cccatgagcg ccggcgcgga gccgcttgag cgtgaagagt gcaaggcact cgaatcgcaa
     3301 aagcgcacaa tgctcacgcc caagatcaag acggctctca agcagggccc agattgggtc
     3361 aagaagaacc tccacgacgc cgcagaaatc gagaaaattc gcgaatatct acaggtggag
     3421 gagaaggtgg ccttccgctg ccggaccgac ggggtgcgca ttcccaagcc cctgccgcca
     3481 ccgcttcccg accgcaagcc ggccgtaccg acatacgtcg ccgacggcgc gcccaagatt
     3541 ctcgcagggg ttgcagccac gtccttccta cccctgcgca acccctctcg atccgatacc
     3601 aagaccgtcg aggccgaccc gcctgcaggc gaggagagcg ccggagaagg cgacattacg
     3661 accgctgccg acgaagaagc cgaagcaggg cctagccaag cgatagcgga ttcggataag
     3721 acagcgcctt ccgagaacaa ggcgacacaa tgagtactgt aattctgctg attcacgtca
     3781 tgatcgctct cgcgatggtg ggcgtcatcc tgctccaacg ctccgaaggc ggcgcgcttg
     3841 gtattggcgg cggtggcggc gccggtggcg gcggcttcat gactggccgc ggcgccggaa
     3901 atgccctgac caagacgacg gccatcctgg cggcatgctt cttcgtcacc agcctgaccc
     3961 tctcgatcct ggcgacccag cagcggggcg acacgccgag catcctgccg tcgggaagcg
     4021 gcggtgaagg tgggctgaac ccgcttcaga ttccgggcac gccagcgccc aaaacatcgg
     4081 atacgcccgc cgcgccgcaa gcaccgcaat cccaataggc gtttcacaca agaaattctg
     4141 ctccaagacg tctccccacc catgatgggg ctacgccttg gcgtggactc ggtataaggt
     4201 cgaggcccat ggcgcggtac gtcttcatta ccggcggcgt ggtttcctca ctaggcaagg
     4261 ggctcgcttc ggcagcgctc ggagcgcttc tacaggcgcg gggctttagc gtccgcctgt
     4321 gcaagctcga cccctacttg aacgtcgatc cgggaaccat gagcccctat cagcacggcg
     4381 aggtgttcgt caccgacgac ggcgctgaga ccgatctgga ccttgggcac tacgaacgct
     4441 tcaccggtcg gccggcgagc caagcagaca acatcaccac gggccgcatc taccaggaga
     4501 tcatcgctaa ggagcgccgc ggcgactatc tcgggtccac ggttcaggtc attccccacg
     4561 tgaccgacgc catcaaggcg ttcgtcacca cgggcaacga ggatgtcgac tttgtcctgt
     4621 gcgagatcgg cggcaccgtg ggcgacatcg agggcctgcc gttcttcgaa gccatccgcc
     4681 agctcggcaa cgagctgcca cgcggccaaa gcgcctatat ccatctaacg ctgctgccgt
     4741 tcatcccgag cgcgggcgaa ctcaagacca agccgacgca gcattccgtc aaggagctgc
     4801 gctccatcgg tatccaaccg gatgtcttgt tgtgccgcgc ggaccggacc attccgcccg
     4861 acgagcgccg caagatcgcg ctgttctgta acgtgcggcc cgaagcggtc gtccaggcgc
     4921 tcgacgtggc gagcatttac gacgtgccga tcgcctatca cgaagagggg ctcgaccggg
     4981 agctgctcgc ggccttcggc attaccgatg cgcccgatcc cgatcttcgc atgtggcggc
     5041 aaatctccga gaccatcgcc gagccggacg gcgaggtgac cgtcgccatt gtcggcaaat
     5101 ataccgggct gcaggacgcc tataagtccc tgaacgaagc cctcgtgcat ggcggcatcg
     5161 ccaacaaggt gaaggtcaat ctggagtgga tcgaggcgca aatcttcgag agccaggacg
     5221 ccgccgctta tctggagaac gtcaacggca ttctcgtgcc gggcgggttc ggtgagcgtg
     5281 gcgccgaggg caagatcaaa gccgcgcgtt ttgcgcgcac caagcagctt ccttatttcg
     5341 gcatctgctt cggcatgcag atggccgtgg tagaagccgc gcgcaacctc gccggcatcg
     5401 aaggcgccag ctccacagag ttcgggcctt cggcagaacc agtgatcggc ctgatgaccg
     5461 aatggatgaa gggcgacgag ctggagcgcc gcgagcagaa cggcaatctc ggcggtacca
     5521 tgcggctcgg tgcctatgac gccgtgctag ccagaggcag caaggtggcg gagatctatg
     5581 gcgacacgga aatctcagaa cgccaccgtc accgctacga ggtcaacatg cgctaccgca
     5641 acaaactcga agctgcgggc ttgcgcttct cagggctttc acccgacggc cttcttccgg
     5701 agatcgtcga gataccggag cacccctggt tcgtaggcgt tcagttccat cccgaact
//
LOCUS       DBNHVU010000067         4998 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1438240, whole genome shotgun sequence.
ACCESSION   DBNHVU010000067 DBNHVU010000000
VERSION     DBNHVU010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1438240"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..701
                     /locus_tag="AC16Y7_02530"
     CDS             111..701
                     /locus_tag="AC16Y7_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009268 - response to pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HIR5708287.1"
                     /translation="MAIPVDAAAAALREAMRETVKRYSLWYLIQGILLVVAGVLALIY
                     PFISSVAIVFLLAWILIVSGILQGIGLIGASNVPHYWLQLISAVLAILIGVLLLRSPD
                     SGLLIMTVLLIVYFMVEGIAKVIFALTIRPFPNWGWVLGSGLVGILLALILWANMPLT
                     SDWVLGLMLGVLLICEGAALTYLAWQVRHAPAVSNG"
     gene            complement(736..2160)
                     /locus_tag="AC16Y7_02535"
     CDS             complement(736..2160)
                     /locus_tag="AC16Y7_02535"
                     /inference="COORDINATES: protein motif:HMM:NF018095.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR5708288.1"
                     /translation="MSNAKPPGLAERLEAERLKAERLDAKRLDAEFPVGLRLAAKAEP
                     TASSMAERFAPQPLRATREETPAVEAVEAVDPTPASLEPRLFTAPRQGVLAILMGVAI
                     APAAILAGLLWFGAIRGPDVGPSADAKPARHQAAFAAAPSLRVAPRAPEFGLTSPEEI
                     MATAGEDVAFTIAIETSEAIPTRSVIAIRDLPDGAAFSQGRPFGSREWSLTPDEIAGL
                     SLHVPNDHAGTSDLAVELVAADGTVLARTATRLDVAPSPTAGLVVRSGEEDRIKDLIA
                     HGHKMIAVGYFAGARAYYQRAAEAGSGEAALAAGATYDPAFIAALRAQGIKPDKQAAQ
                     EWYGRAAALGITDRAGQLATLRQTWMNAGASPEGDASPAGDPKPAEQTGPTTQAEAER
                     GPFGRLVAAATELASGDEWVVVANPVHVRKGPSSTDETFKVAQKGTKLRVLGRDGNWV
                     QITDPATKQQGWIYKRFLKEAAAP"
     gene            complement(2258..3439)
                     /locus_tag="AC16Y7_02540"
     CDS             complement(2258..3439)
                     /locus_tag="AC16Y7_02540"
                     /inference="COORDINATES: protein motif:HMM:NF028178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR5708289.1"
                     /translation="MMRPALAIGLCLLPFVAADLMSDSTAYAKDRRAKAQVAVQTKQR
                     TPQRAVRGRYRAELNDNTVTIMGGSATGTDAAVIQDIAAVLDEGTAMRVVPMIGKGPA
                     QTLKDVMFMRGVDMGITQANVLKHFANTGELGPLDKQIAYVAKLFNEEMHVLAHSGID
                     DLTALNGRVVSIGPEGSGLELTARAVFAALGVEVRETQLDEADALVKLKAGEIDAMVV
                     IAGKPAPSLAHLGRDSGLKFLGLPYPKGLEAEYYPATLTHDEYPALIEKGAQVDTVAV
                     CAVLVSFNWSDDNIRKKKLARFVDHFFSNFDAFRVSPRHPKWRQVNFAATLEGWRRSP
                     LAQSWIDQAKTAVAAGGQTQERFETFLAQADAGRTQVSETERVKLFRAFLEWSKTQKQ
                     N"
     gene            complement(3893..4093)
                     /locus_tag="AC16Y7_02545"
     CDS             complement(3893..4093)
                     /locus_tag="AC16Y7_02545"
                     /inference="COORDINATES: protein motif:HMM:NF019057.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HIR5708290.1"
                     /translation="MKFEIFKAFNGQYYWRGRGGDGKTLCHSEGYRHKEAAEHAIQAI
                     RGEAASAAIDDLTQAKAAGFGH"
     gene            complement(4148..>4998)
                     /locus_tag="AC16Y7_02550"
     CDS             complement(4148..>4998)
                     /locus_tag="AC16Y7_02550"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4 family protein"
                     /protein_id="HIR5708291.1"
                     /translation="KLKLNDASLRYEPEVSQALGFGFRCGFLGLLHLEIIRERLEREF
                     NIDLIATAPSVIYRVFLTDGSMVELHNPADMPELQKVERIEEPWIEATILVPDEHLGA
                     VLKLCEDRRGRQKTLSYAGSRAMVVYELPLNEVVFDFYDRLKSVSRGYASFDYHITGY
                     EQGDLVKMALLVNAEPVDALSVIVNRTRSEARGRAMVEKLKDLIPRHMFKIPIQAAIG
                     GKVIARETISALRKDVTAKCYGGDITRKRKLLEKQKAGKKKMRSFGKVDIPQEAFIAA
                     LKMDAD"
BASE COUNT          815 a         1627 c         1574 g          982 t
ORIGIN      
        1 aggtacccgt ctgaagtctg ctcgtggtcc atgtgccccc gtgaatcgcg tatgcttggc
       61 acgcggccgc gcgattgatt cggccgccaa cctagaggag ggctctaccc atggctattc
      121 cggtcgacgc cgcagcggcg gcgctccgtg aagctatgcg tgagacggtg aagcgttatt
      181 cgctctggta tttgatccag gggatcttgc tggtggtggc gggcgtgctc gcgctgatct
      241 acccgttcat ctcctcggtc gccatcgtct tcttgcttgc ctggatcctc atcgtcagcg
      301 gcatcttgca gggcatcggg ctcatcggcg ccagcaacgt gccccattat tggctgcagc
      361 tgatctccgc ggtgctggcc attctgatcg gcgtgctcct gctgcgctcg cccgatagcg
      421 gcctgctcat catgaccgtg ctgctgatcg tctattttat ggtcgaaggc atcgccaagg
      481 tgatcttcgc gctcaccatc cggcccttcc ccaattgggg ctgggtgctc ggcagcggcc
      541 tggtcggcat tctactggcg ctcattctct gggcgaacat gccgctcaca tccgactggg
      601 tgttggggct gatgcttggc gtgctgctca tttgcgaagg cgctgcgctg acctacctcg
      661 cctggcaagt gcgccatgca ccggcagtgt cgaacggcta aggcgccgcc agccggtaaa
      721 ggcgctgcca gccccttagg gcgcggcggc ctcttttagg aagcgcttgt agatccaccc
      781 ttgctgcttg gtggccgggt cggtgatctg cacccagttt ccgtcgcggc ccagcacgcg
      841 cagcttggtg cccttctggg cgaccttgaa cgtctcgtcc gtcgaggagg gtcctttgcg
      901 gacgtgcaca gggttggcca ctacgaccca ctcatcgccg ctggcaagct cggtggcggc
      961 ggctacgagc cgcccgaagg gcccgcgctc tgcctcggcc tgtgtggttg ggcccgtttg
     1021 ctcggcgggc ttgggatccc ccgccggcga cgcgtctcct tccgggctcg ctccggcatt
     1081 catccatgtt tgcctcagcg tggcgagctg acctgcgcgg tcggtgatgc cgagcgccgc
     1141 ggcgcggcca taccactcct gcgccgcttg cttgtcaggc ttgatgcctt gcgcacggag
     1201 cgccgcgatg aacgcgggat cgtacgtggc gcccgcggcg agcgctgcct ccccgctgcc
     1261 tgcttccgct gcgcgctggt agtaagcgcg cgcaccggcg aagtagccca cggcaatcat
     1321 tttgtggccg tgggctatca gatccttgat gcgatcctct tcgccggacc gtacgacgag
     1381 accagccgtg ggcgagggag ccacgtccag ccgggtcgcc gtacgggcca gcacggtgcc
     1441 gtcggcggcg acaagctcga cggcaagatc cgaagtcccg gcgtgatcgt tcggcacatg
     1501 caaggagagg ccggcgattt catccggggt gaggctccat tcgcgcgagc cgaacgggcg
     1561 cccttgcgag aaggccgcgc cgtcgggaag atcgcgaatg gcgatcacgc tgcgcgtggg
     1621 gatggcctcg cttgtttcga tggcaatagt gaaagcgacg tcctcgccgg cagtcgccat
     1681 gatttcctcg ggcgaggtca ggccgaactc cggcgcccgc ggggcaacgc gcaagctagg
     1741 cgcggccgcg aacgcagcct ggtgacgtgc cggcttggcg tcggcgctgg gcccgacgtc
     1801 aggtccgcga attgcgccga accataaaag cccggcaagg atggcggccg gcgcgatggc
     1861 cacgcccatc aggatcgcga gcacgccctg ccgcggtgcg gtgaacaggc gcggttcgag
     1921 cgaggcaggc gtcgggtcca ccgcctccac cgcctccacc gctggcgttt cttctcgtgt
     1981 tgcgcgcaag ggctgcggcg caaagcgctc ggccattgac gatgcggtcg gctccgcctt
     2041 tgccgctaga cgcaacccaa ccggaaactc cgcgtcaaga cgcttcgcat cgagccgctc
     2101 cgccttgaga cgctccgcct caagacgctc cgccaggcct ggcggcttgg cattcgacat
     2161 gtgtttcgaa tctcggtcct ttggactgcc aaccgtcctg aacagcggct gcaacatggc
     2221 accgtcctcc ccacgcaata gtcaacaaag aaggcgacta gttctgcttc tgcgtcttgc
     2281 tccactccaa aaaggcacga aatagcttca cgcgctccgt ctcggacact tgggtacgcc
     2341 cagcgtccgc ctgagccaga aatgtctcga agcgctcttg cgtctgcccg ccggcggcga
     2401 ccgcggtctt cgcctggtcg atccaactct gcgccagcgg ggatcgccgc cagccttcca
     2461 aagttgcggc aaagttcacc tgccgccact tgggatggcg cgggctcacc cgaaacgcat
     2521 cgaagttcga aaagaagtgg tcgacgaagc gcgcgagctt cttcttgcgg atgttgtcgt
     2581 ccgaccaatt gaacgacacc agcacggcgc acaccgccac cgtgtccacc tgcgcgccct
     2641 tttcgatcag cgccgggtac tcgtcgtggg tcagcgtcgc cgggtagtac tcagcctcca
     2701 ggcccttcgg gtagggcagg ccaagaaact tgagcccgct gtcgcggccg agatgggcca
     2761 ggctcggcgc cggcttgccg gcgatcacca ccatggcgtc gatctcgccc gctttgagtt
     2821 tgacgagcgc gtcagcctca tcgagctgcg tttcgcgcac ctcaacgccg agtgcggcga
     2881 acaccgcgcg cgcggtgagc tccaggccgc tgccttcagg gccaatactg acgaccctgc
     2941 cgttcagtgc tgtgaggtcg tcgatgccgg aatgcgccag cacgtgcatc tcctcgttga
     3001 acagtttcgc gacataggcg atctgcttgt ccagcggacc gagttcgccg gtgttggcga
     3061 aatgcttcag gacattcgcc tgggtaatgc ccatgtcgac accgcgcatg aacatcacgt
     3121 ccttcagggt ctgcgccggc cccttgccga tcatgggcac cacgcgcatc gcggtccctt
     3181 catcgagcac ggcggcaata tcctggatca cggcggcgtc ggtgcccgtc gcgctgccgc
     3241 ccatgatggt cacggtgttg tcgttgagct cggcgcggta gcgcccgcga acggcgcgct
     3301 gtggcgtccg ttgcttggtc tggacggcca cttgtgcctt ggcccggcgg tctttggcgt
     3361 aggccgtgct gtcgctcatc aagtccgccg ccacgaacgg cagaaggcaa agtccaatcg
     3421 cgagggcggg acgcatcatg acccctccgg aacagcgctt caccccgacc taaggacccc
     3481 ttgcgcgaag cgcgggaaga caagacggcc cctgtgcagc cacatgcttg ttgcttgggg
     3541 tgtcgcgcct ggcgtcagcc aacgacatcc cccgtcgtgc tgcttttcct tgggccgaat
     3601 ggaaagtgat atattagccg ctgtcaagca tagatgcgat aatgacacaa caaaatcaag
     3661 gtattgtcaa taataaggca acgacaacta gaaaatggca agatgaaggc aagtcattag
     3721 ggaagtgtta accacaaaca attgcgcacc aacatcataa agacgtgacg cgatcttggc
     3781 acagcagtag gcgcgggcgc gccattggcg gcgcgcccac tcataacgtc gaacttggca
     3841 attggtttcg tgatcggggg cgatcgaaat ccagccccgc ccggacccgc ctctagtggc
     3901 cgaaccctgc ggccttggcc tgggtgaggt cgtcgatcgc ggcggaggcc gcttcaccgc
     3961 ggatcgcctg gatcgcgtgc tcggccgcct ctttgtggcg atagccctcg gagtggcaca
     4021 gggtcttgcc gtcgccgcca cggccacgcc agtagtactg cccattgaag gctttgaaga
     4081 tttcgaattt cattttcgta ggtctccatt ctgctggatg agcctacacc cggcttgagg
     4141 gcttcaccta gtccgcgtcc atcttcagcg cggcgatgaa ggcctcttgc gggatgtcga
     4201 ccttgccgaa gctgcgcatc ttctttttgc ccgccttctg cttctccagc agtttgcgct
     4261 tacgggtgat gtcgccgcca tagcacttgg ccgtcacgtc tttgcgcagc gcgctgatcg
     4321 tttcgcgcgc gatgaccttg ccgccgatcg ccgcttggat cgggatcttg aacatgtgcc
     4381 gggggatcag atccttcagc ttctccacca tggcgcggcc gcgcgcctcg gagcgcgtgc
     4441 ggttgacgat gacagagagc gcgtcgaccg gctcggcgtt gacgaggagc gccatcttca
     4501 cgaggtcgcc ctgctcatag ccggtgatgt ggtagtcgaa cgaggcgtag ccgcgcgaca
     4561 ccgatttgag ccggtcgtag aagtcgaaca cgacttcgtt cagcggcagc tcatagacca
     4621 ccatggcgcg cgagcccgca tagctcagcg tcttctgccg cccgcgccgg tcctcgcaaa
     4681 gcttcagcac cgcaccgaga tgctcgtccg gcacgagtat ggtcgcctct atccacggct
     4741 cctcgatgcg ctcgaccttc tgcagctcgg gcatgtcggc cggattgtgc aattcgacca
     4801 tgctgccgtc ggtgaggaac acgcggtaga tgacggacgg cgccgtggcg atcaggtcga
     4861 tgttgaactc gcgctccagc cgttcgcgga tgatctccag gtgtaggagg ccgagaaacc
     4921 cgcagcggaa gccgaagccg agcgcctgcg aaacctcggg ctcgtaacgc agcgacgcat
     4981 cgttgagctt gagtttct
//
LOCUS       DBNHVU010000068         4665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_2033114, whole genome shotgun sequence.
ACCESSION   DBNHVU010000068 DBNHVU010000000
VERSION     DBNHVU010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_2033114"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1301
                     /locus_tag="AC16Y7_02555"
     CDS             <1..1301
                     /locus_tag="AC16Y7_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR5708292.1"
                     /translation="GQVIEGVVKNITDYGAFIDLGGIDGLLHVTDMAWRRVNHPSEIV
                     NVGDSVKVQIIRINTETQRISLGIKQLESDPWEGIAAKYPVDSRFKGTVTNITDYGAF
                     VELEPGVEGLIHVSEMSWTKKNIHPGKIISTSQEVEVQVLEVDPDKRRISLGLKQTQE
                     NPWIAFAGGHKAGAEIEGQVRNITEFGLFVGLDGGIDGMVHLSDLDWSKSGEEALKDY
                     KKGDTVKAVVLEADAEKERISLGIKQLTPDPFEGEAASGKLKKGVRVTCEVTAVKDNG
                     IEAKLIDQDLTTFIRRSDLSRDRSEQRPDRFGVGEKFDAVVTQVDPKTRKLTASIKAL
                     EIAEEKEAVAQYGSTDSGASLGDILGAALNKASGDVEEEPAEKPKPKRKTAAKAKADD
                     EAAEAEASSEEEPAEKPKRKRAAKAKTDDEAADAKASSEE"
     gene            1335..2297
                     /gene="sppA"
                     /locus_tag="AC16Y7_02560"
     CDS             1335..2297
                     /gene="sppA"
                     /locus_tag="AC16Y7_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HIR5708293.1"
                     /translation="MVLDAERVIEHRRLKRRVTAWRVAAVVLGLLALGVIIFSDKQMA
                     GSAGILPHIARINVSGVITDDRKMLQLIDRVAKANQVRAVILDINSPGGTTTGGEAMY
                     DAIRRLTEKKPVVATCGTLATSAAYIVALATDRIFVYGNTITGSVGVIFQWANVTELL
                     KTVGIEVEEVKSGPLKAVPSPFEPTDERAREMTAEMVEDAKVWFVDLVKERRKISPGS
                     VPGLTEGRIYSGRQAVNLKLVDQIGGERAALAWLNKEHGIPTRLKVVQWQPKDESAGF
                     FGLLFDSASAILGVSGGKLALILDRASASLNLDGLVSVWHPASN"
     gene            2349..2648
                     /gene="ihfB"
                     /locus_tag="AC16Y7_02565"
     CDS             2349..2648
                     /gene="ihfB"
                     /locus_tag="AC16Y7_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HIR5708294.1"
                     /translation="MIKSELIQRVAAANPHLFHRDVERIINIVLDEITGALARGDRVE
                     LRGFGAFTVKHRAARQGRNPRTGDTVFVEDKYVPFFKTGKELRERLNPNSGDSQS"
     gene            2676..3023
                     /locus_tag="AC16Y7_02570"
     CDS             2676..3023
                     /locus_tag="AC16Y7_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HIR5708295.1"
                     /translation="MWRKLILALIVVPLGVVLIALAVVNRDPAVLILNPFGGAEPGLS
                     LQAPFFLFLLCAFAIGLVVGGIASWLNQGKWRRTAREEAREARDWRRQADRLEKELED
                     LHPAGQRPQLPAE"
     gene            3034..3267
                     /locus_tag="AC16Y7_02575"
     CDS             3034..3267
                     /locus_tag="AC16Y7_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708296.1"
                     /translation="MDPLTVFGLLAVTAMLVTYALEERSHWYILAFAGACLLGSAYGF
                     LQGAWPFGVLEAVWAGVAAWRWRERASAASGSH"
     gene            3388..4044
                     /locus_tag="AC16Y7_02580"
     CDS             3388..4044
                     /locus_tag="AC16Y7_02580"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR5708297.1"
                     /translation="MTVKVKICGVRTPAILDAAAGAGADYVGLMFFPKSPRNVGLDEA
                     AALAAAARGRVRTVAVMVDPDDALVDAVVEQVRPDALQFHGSETPERVAAIKARTGLA
                     VIKAIHVGEAADVARADAYEAVDHILYDAKAKPGAPLPGGNGVAFDWSVLAGARAPFA
                     LSGGLAPDNVAEAVRQTGAAMVDVSSGVETAPGVKDADLVTQFIAAARGAAPIKAKAS
                     "
     gene            4041..>4665
                     /locus_tag="AC16Y7_02585"
     CDS             4041..>4665
                     /locus_tag="AC16Y7_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR5708298.1"
                     /translation="MTTITDALNTYREGPDERGHFGLFGGRFVAETLMPLILDLEQAY
                     EDAKADPSFRAELDALNAHYAGRPSPLYFAERLTEHLGGAKIYFKRDELNHTGSHKIN
                     NCLGQIQLAKRMGKTRIIAETGAGQHGVAVATVCAKFELPCEVYMGATDVERQAPNVA
                     RMKLLGAKVNPVTSGAGTLKDAMNEALRDWVTNVRETYYIIGTAAGPH"
BASE COUNT          857 a         1457 c         1559 g          792 t
ORIGIN      
        1 agggccaagt catcgaaggc gtggtcaaga acatcaccga ttacggcgcc tttatcgatc
       61 tgggcggcat cgacggtctg ctgcatgtca ccgacatggc gtggcggcgc gtgaaccatc
      121 cgagcgaaat cgtgaatgtc ggcgattccg tgaaggtgca gatcatccgc atcaatacgg
      181 agacccagcg catcagcctc ggcatcaagc agcttgagtc cgatccgtgg gaaggcatcg
      241 ctgccaagta cccggtcgac tcgcgcttca agggcaccgt caccaacatc accgactacg
      301 gcgccttcgt ggagctggag cccggcgttg agggtctgat ccacgtttcg gagatgagct
      361 ggacgaagaa gaatattcac cccggcaaga tcatttccac gagccaagag gtcgaggtgc
      421 aagtgcttga ggtcgatccc gacaagcggc gcatttcctt gggcctgaag cagacccagg
      481 aaaatccgtg gatcgccttt gccggcggcc acaaagccgg agctgagatc gaaggtcagg
      541 ttcgcaacat caccgagttc ggcctgttcg tcggcctaga tggcggcatc gacggcatgg
      601 tgcacctgtc ggatctcgat tggtccaagt ctggggagga agccctcaaa gattacaaga
      661 agggcgacac ggtgaaggcc gtggtgctgg aggcagacgc cgaaaaggag cgcatcagcc
      721 ttggcatcaa gcagctcacg cccgatccgt tcgagggcga agcggcctcc ggtaagctca
      781 agaaaggcgt gcgcgtcact tgcgaggtca ccgccgtcaa ggacaacggc atcgaggcca
      841 agctcatcga ccaggacctc accaccttca ttcgccgttc cgatttgtcg cgggaccggt
      901 ccgagcagcg ccccgaccgt ttcggcgtcg gcgagaagtt cgacgctgtg gtcacgcaag
      961 tcgatcccaa gacacgcaag ctcactgcgt ccatcaaggc gctcgagatc gccgaagaga
     1021 aggaggccgt cgcccagtac ggctcgacgg attccggtgc gtctctcggc gacattctcg
     1081 gcgcggccct gaacaaggcg agcggcgacg tcgaagagga gcctgccgaa aagcccaagc
     1141 ccaagcgcaa gaccgcggcc aaggccaagg cagacgacga agcggcggag gccgaggcgt
     1201 cgtccgaaga ggagcctgcc gaaaagccga agcgcaagcg tgccgccaag gccaagacag
     1261 acgacgaagc agcggatgcc aaggcatcgt ccgaagagta ggcgaggtcg tgacccggag
     1321 ggtctgagca agccatggtt ctcgacgccg aaagagtgat cgagcaccgc cgcctcaagc
     1381 ggcgggtcac cgcctggcgc gttgccgcgg tggtgctggg cctattggcc ttgggcgtga
     1441 tcatattttc cgacaagcag atggcggggt ccgccggcat tcttccccac atcgcgcgca
     1501 tcaacgtgag cggcgtcatc accgacgacc gcaagatgct ccagctgatc gatcgggtgg
     1561 cgaaggccaa ccaggtccgc gcggtgatcc tcgacatcaa cagccccggc ggcaccacca
     1621 cgggtgggga ggcgatgtac gatgccattc gccgccttac cgagaagaag cccgtcgttg
     1681 cgacgtgcgg gacgctcgcc acatccgccg cctatatcgt ggcgctcgcc acggatcgca
     1741 tctttgtcta cgggaacacc atcaccgggt ccgtcggcgt catcttccag tgggctaacg
     1801 tcaccgagct gctcaagacg gtcggtatcg aggtcgagga ggtcaagagt ggtcccttga
     1861 aggcggtgcc gagcccgttc gagcccacgg atgaacgcgc tcgcgagatg acggccgaga
     1921 tggtcgagga cgccaaagtg tggttcgtcg atctggtgaa ggagcgccgc aagatctcgc
     1981 cgggatcggt tccgggtctc accgaggggc gcatctattc cggccgccag gctgtgaatt
     2041 tgaagcttgt cgaccagatc ggcggggagc gcgcggccct cgcctggttg aacaaggagc
     2101 acggaatccc gacccggcta aaggtcgtgc aatggcagcc aaaagacgag tcggcgggct
     2161 ttttcggcct gctgttcgac tccgccagcg cgattctggg cgtgtccggc ggcaagctag
     2221 ccttaattct cgaccgggca tccgccagcc tgaaccttga cggtcttgtc tccgtttggc
     2281 accctgcatc caactagtgg gggcgtaggg ctagcggcaa ccaatggctg cagccccgtg
     2341 gggcgtgaat gatcaaatcc gagctgattc agcgggtggc agccgccaat ccgcatctat
     2401 ttcatcgtga tgttgagcgt atcatcaaca ttgtgctcga tgagatcacg ggcgcgctgg
     2461 cgcgcggcga tcgcgtggag ttgcgtggtt tcggcgcatt tacggtgaag caccgtgccg
     2521 cccgtcaggg ccgtaacccg cgcacgggcg atacggtctt cgtcgaggac aagtacgtac
     2581 ccttcttcaa gaccggcaaa gaactacgtg agcgcctgaa cccgaacagc ggtgacagcc
     2641 agtcgtaggc tcgccggccg cttatggagc cgctcgtgtg gcgtaagctc atcctcgcac
     2701 tcatcgtcgt gccgttgggc gtggtgctca tcgcgttggc cgtggtcaat cgcgatccgg
     2761 cggtcctgat cctcaacccc tttggtggcg cagagcctgg tctctcgctt caggcgccgt
     2821 tcttcttgtt tctgctctgt gcgttcgcga tcggcctcgt ggtgggcggc atcgcctcct
     2881 ggctcaacca gggcaagtgg cgccgcaccg cgcgcgagga ggcccgggag gcccgcgact
     2941 ggcggcgtca ggcggaccgc ctggagaagg agctggaaga cctccatccg gcagggcagc
     3001 ggccgcaact gccggccgag tagcgggctg cggatggacc cgctcacggt tttcggtctc
     3061 ctcgcggtca ccgcgatgct ggtcacctac gccctggagg agaggagcca ttggtacatc
     3121 ctggccttcg ccggcgcttg cctgctgggc tcggcctacg gcttcctgca aggggcctgg
     3181 cccttcggtg tcctggaggc cgtctgggcg ggcgttgcgg cttggcgctg gcgcgagagg
     3241 gcgtctgcgg cctcagggtc gcattgagcc tccggtaacg ccgatgttgc agtgcgatgg
     3301 acttgaagcc ccggatttca tagggtgagc ccgcgataag gcccgaaaag gcccgcgaaa
     3361 ccctcacccg agagccaata gagccccgtg acggtcaagg tcaaaatttg tggcgtgcga
     3421 acgcccgcca ttctcgacgc cgccgccggc gccggcgcgg actatgtcgg gctcatgttc
     3481 ttccccaaga gcccgcgcaa tgtcggcctg gacgaggccg cggcgcttgc cgcggctgcc
     3541 cggggccgtg tgcgcacggt cgccgtcatg gtcgatccgg acgatgctct ggtcgatgcc
     3601 gtggtggagc aggtgcgccc cgatgcgcta cagttccatg gctccgagac ccctgaacgc
     3661 gtggctgcca tcaaggcgcg cacggggttg gcggtgatca aggcaatcca cgtcggtgag
     3721 gcggcggatg tcgcccgcgc cgacgcctac gaggccgtcg accacattct ttacgacgcc
     3781 aaggccaagc caggcgcgcc gctgccgggc ggcaacgggg tcgctttcga ctggagtgtg
     3841 cttgcgggcg cccgcgcgcc gttcgccctg tcgggagggc tcgccccaga caatgtcgct
     3901 gaagccgtcc gtcagacggg cgccgcgatg gtcgatgtct cttctggggt cgagacagcc
     3961 cccggcgtga aggacgctga cctcgttacc cagttcatcg cggcggcgcg tggcgccgcc
     4021 cctattaaag ccaaggcatc atgacgacga ttaccgacgc actgaatacc tatcgcgagg
     4081 gtcccgacga gcgcggccat ttcggcctgt tcggcggccg gttcgttgcc gagactctga
     4141 tgccgctgat cctcgatctg gagcaggcct acgaggatgc caaggccgac ccgtcctttc
     4201 gggccgagct cgatgcgctc aacgcccact atgcggggcg cccgagcccg ctctacttcg
     4261 ccgagcggct gacggagcat ctcggcggcg ccaagattta ctttaagcgc gatgagctga
     4321 accacacggg ctctcacaag atcaacaact gcctcgggca gatccagctc gccaagcgca
     4381 tgggcaagac gcggatcatc gccgagaccg gggcgggtca gcatggtgtg gccgtggcga
     4441 cggtgtgcgc caagttcgag ctgccgtgcg aggtctacat gggcgccacg gacgtggagc
     4501 ggcaggcgcc gaacgtggcg cgcatgaagt tgctcggcgc caaggtgaac ccggtgacgt
     4561 cgggcgcggg cacgctgaag gacgcgatga acgaggcgct ccgcgactgg gtgaccaacg
     4621 tgcgggagac ctattacatc atcggcacgg ccgcggggcc gcacc
//
LOCUS       DBNHVU010000069        14876 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_770457, whole genome shotgun sequence.
ACCESSION   DBNHVU010000069 DBNHVU010000000
VERSION     DBNHVU010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 14876)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14876)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14876
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_770457"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="AC16Y7_02590"
     CDS             <1..295
                     /locus_tag="AC16Y7_02590"
                     /inference="COORDINATES: protein motif:HMM:NF021589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR5708299.1"
                     /translation="TTTLVDSLGAIDRFYELSLQQAIWRYLVLGTFVSYEIADYAATP
                     QVDQRFGTGASAEYYFNPYMSVYTEYDYRDFFSNNEFDDFTENRVRIGMRIRR"
     gene            complement(292..1164)
                     /gene="galU"
                     /locus_tag="AC16Y7_02595"
     CDS             complement(292..1164)
                     /gene="galU"
                     /locus_tag="AC16Y7_02595"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HIR5708300.1"
                     /translation="MTARVRKAVFPVAGLGTRFLPATKAMPKEMLTVVDRPLIQHVVD
                     EACAAGIEPVIFVPGRTKGVIEDHFDMQPELEGTLRARGKTAELKLLSEALPEAGSAS
                     FVRQQAPLGLGHAVWCARDIVGDEPFAVVLPDVLVQSPGKGCLAQMLDVYDKHGGNIL
                     AVEPVPDDELKSYGVVAVGDADRNVFPVTGMVEKPAPGTAPSNLTILGRYVLQPEIFG
                     LLGEQEKGAGGEIQLTDSMLALLKSQSFYAVEYEGRAFDCGSKVGFLAANVAFALERP
                     DLRDAVAVEIKKLI"
     gene            1442..2722
                     /locus_tag="AC16Y7_02600"
     CDS             1442..2722
                     /locus_tag="AC16Y7_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018235258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HIR5708301.1"
                     /translation="MSALTLAALVLLPVPTKADAVSDYRRAVDARFGQWLQDLWPEAE
                     AAGVSRATFDREIKGLKLDWSLPQIEPPDPAVPGGPALPAAMKPKPKPQAEFGVPQNY
                     FKARSLNGLAAAGRARSKKLAPTLKAIEAQYGVPGSVVLAIWGRETGFGRAALPHDAL
                     TAIATQAFMGRRADEFRPQLIGALKIIELKHATRAQMKSSWAGAMGHVQFLPGDVENY
                     AVDFDGNGRRDIWNSVPDSLASAANSLKSQGWDGNQPWAYEVTLPKNFDCTLQGPDQS
                     LPIREWIDLGVERVKGRPFPKERLEDWTFLVLPAGLKGPAFLATTNFSVLKLYNNSDV
                     YAMFVGHVADMIAYGSPAAFVGKWKPVERFTRDRILKFQNVLVAEGHDVGKVDGLVGF
                     KTRQSIGKKEKELGKPLTCYPSHALVNQVLKAAN"
     gene            2799..4394
                     /locus_tag="AC16Y7_02605"
     CDS             2799..4394
                     /locus_tag="AC16Y7_02605"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HIR5708302.1"
                     /translation="MNISKALMFGAVSAFLLAPSGPAHADRFQNCVKGFWPAAKRAGV
                     SRDTFQRATAGITRDKEVIEAAKYQPEYKKPIGEYVDRAISPKRLATGKEKLIEYKPL
                     LDQVEAKYGVDRHIVVAIWGVESNYGDNKGEKGVIQSLMTLACSGTKAKFARGQIVSA
                     LKVLQRGDTTFENMNGSWAGAMGHTQFIPTTYTAYAVDFDGDGRRDIWDTIPDALGST
                     AAYLRKSRWKPGQTWGYEVKLPKKFNPKRVSRKTYRTVAQWQKAGIKRINGKPFPRPG
                     DKAQLFAPEGKNGPAFLLLNNFRSLLRYNQADSYALAVGHLADRLAGYGPFVQAWPTG
                     ERRLSMEQRMELQRLLIALGHLEGDVDGIIGSGTLEGVRSYQRVKKLPVDGYPTQTIL
                     KKLRADAPPLPPVADPEPIGAVTPPPADPQSAAPQPVGAANQQAAPAMPAPAQAPAAP
                     APAAAVPAPAAPAAPAAAVPAPTAAAAPAAAAPAMPAPPASVAPPQAPAVPANQPASV
                     VVPKSAPAGQPVTMTLERAPSAN"
     gene            4437..5666
                     /locus_tag="AC16Y7_02610"
     CDS             4437..5666
                     /locus_tag="AC16Y7_02610"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016218.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR5708303.1"
                     /translation="MVLSAFGLRLTRALIGAAAFGLLPALMLGFSGQSVLAQTNQTAH
                     RSYITPFPNGDRYRIVVVGDSLGEGLWGGLYRAFEADPTLDFIQRSQKSTGFVNTRRY
                     NWNGEIDKIMKDGNYQVAVAMFGANDAQPIKQDGKVLKVGTEEWREAYGARVEKFIKK
                     LRAANVAVYWVGLPVMRSPGQSSNAEKLNEVFREKAFVNGAKFIDTWEGFADEQGRYS
                     AYGPDMSGKSKRLRANDGKHFTMRGNVKLAHFVEKELRKDINLAKAERNIPLAGNEQE
                     QAKVKDRFVAPKPVAPKKTQNVAEGGEEEDALRESQVGDVSVVRPTALPSVAAPADRL
                     TAQQAATTTEPEVITSQLPGGFTSMATISAVTDLTLASSRPQLPLKQRPYYRVLIQGE
                     QLEPKSGRADDFAWPPS"
     gene            complement(5687..7102)
                     /locus_tag="AC16Y7_02615"
     CDS             complement(5687..7102)
                     /locus_tag="AC16Y7_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HIR5708304.1"
                     /translation="MGKITGFLEIERQERGYEPAADRVRHFKEFVIAPGEAELVRQAA
                     RCMDCGIPYCHTGCPVNNQIPDWNDLVYQDDWLDATRNLHSTNNFPEVTGRICPAPCE
                     AACTLNIIDEPVTIKSIECAIADRAFSEGWVKPQPPEKTTGKRVAIVGSGPAGLAAAQ
                     QLARAGHDVHVYEKHAEPGGLLRYGIPDFKMEKYVIERRVAQMQAEGVTFHCNVNVGK
                     DVAIGELERGHDAVLLAGGSEAPRDLAVPGRDLPGVHFAMTFLPQQNRRVSGEPLGDV
                     EEIVADGKQVVVIGGGDTGSDCIGTSFRQGATSVTQLEIMPEPPEMENKALTWPHWPL
                     KFRTSSSQAEGAERDFSVATNRFLGDGKVEQLECVRLDDQMKPIPDSEFTIPADLVFL
                     AMGFLHPVHKGMIEDLGVDLDQRGNVKANELDYRTSRPKVFAAGDMRRGQSLVVWAIR
                     EGRQAAHAIDKLLMGTTTLPR"
     gene            complement(7252..11961)
                     /gene="gltB"
                     /locus_tag="AC16Y7_02620"
     CDS             complement(7252..11961)
                     /gene="gltB"
                     /locus_tag="AC16Y7_02620"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HIR5708305.1"
                     /translation="MAPRALIPGLTDVAPKGLPRAQGLYDPANEHDACGVGFIADMKG
                     RKSHKIIEQGLQILENLTHRGAAGADPRDGDGAGMLVQIPHAFFEEVCAPLNIKLPEP
                     GRYAVGQIFMPSNQAQRIYCESAIERVIEAEGLQLLGWRTVPTDNATLSPEVVETEPM
                     HRQVFIGRGPGIEDETEFERRLYLLRKVISNTINGETGGRDIGFYIVSLSCRTLVYKG
                     MFLAYQLGAYYPDLHHPKFASALALVHQRFSTNTFPSWKLSHPYRMVAHNGEINTLRG
                     NVNWMAARQASVSSPLFGEDISKLWPISYEGQSDTACFDNALEFLVQGGYSLAHAAMM
                     LIPEAWAGNPLMDAKRRAFYEYHACLMEPWDGPAAMAFTDGRQIGATLDRKGLRPARY
                     FVTDDGLVVMASETGVLPFAEEKIVEKWRLQPGKMLLIDLEKGRIISDEEIKAELAGA
                     NPYQQWLDRTQIHVRELPGSPAPPARTNVSLLDRQQAFGYTQESAKFMMIPMIQTGQE
                     PIGSMGTDTPIAPLSDRPKLLHTYFKQNFAQVTNPPIDPIREELVMSLVTFIGPRPNL
                     LNLEGTLKDKRLEAAHPILTNENLERIRAIGAVADNQFLTVTLDMTYPVEDGADGMAG
                     ALETLCEEAEKAVREGDNIIILSDRATGPDRIPIPSLLATSAVHHHLIREGLRTSVGL
                     VVETGEAHEVNQFCTLAGYGAEAINPYLAFETIEAIVPELDEDLTVEEAITRYMKAVD
                     KGTLKVMSKMGISTYQSYCGAQIFDAVGLHSDFVAKYFTGTNTQIEGVGLEEIAGETV
                     ERHKLAFSDAPVLKNALDVGGEYAFRVRGEAHTWRPEVVADLQHAVRGELPEKYREYA
                     EAVNEQSQQLLTLRGLFRIKNADEIGRTPIPLDEVEPVSEIVKRFATGAMSFGSISRE
                     AHTTLAIAMNRIGGRSNTGEGGEEVDRFTPMENGDSMRSAIKQVASGRFGVTTEYLVN
                     ADMMQIKMAQGAKPGEGGQLPGHKVDATIAKVRHSTPGVGLISPPPHHDIYSIEDLKQ
                     LIFDLKNVNPQGDVSVKLVSEVGVGTVAAGVAKAMADHVTIAGFEGGTGASPLTSIKH
                     AGSPWEIGLAETQQTLVLNRLRGRIAVQVDGGLRTGRDVVVGALLGADEFGFSTAPLI
                     AAGCIMMRKCHLNTCPVGIATQDPVLRKRFTGTPEHVINYFFYVAEEVREYMAALGFG
                     SFDEMIGRSEVLDKEKAIDHWKARGLDFTKLFHKPDVAASLPIRNTERQDHGLESVLD
                     MKLIELCRDALEEKKPVKLDLPIKNVDRSTGAMLSGEVAKRYGHAGLSDDTIWIRFKG
                     SAGQAFGAWLANGVTLDLVGEANDYVGKGLSGGRIIVRPPEESGIDPEKSIIVGNTVL
                     YGAIAGECYFRGVGGERFGVRNSGAIAVVEGTGDHGCEYMTGGVVVVIGPTGRNFAAG
                     MSGGIAYVLDESGDFEQRCNMAMVDLEPIPGENQAMEQQRHQGGDLESHGLVDLTDMT
                     RHDEERLHRLIENHLHYTGSTRAREILDNWATYRPKFIKVMPVEYRRALQEMARQQAA
                     DATSLGEIEIGLRGNGK"
     gene            12402..12710
                     /locus_tag="AC16Y7_02625"
     CDS             12402..12710
                     /locus_tag="AC16Y7_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018064855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BufA1 family periplasmic bufferin-type
                     metallophore"
                     /protein_id="HIR5708306.1"
                     /translation="MTDQSKTITGLALASAFAAALGLAATSAAAEDKKKQVECYGVAK
                     AGDNDCAAGGDTTCAGSSTVDYQGNAWKLVPEGTCTTMELPDGRKGSLKPLDRDLPPV
                     "
     gene            12717..13607
                     /gene="bufB"
                     /locus_tag="AC16Y7_02630"
     CDS             12717..13607
                     /gene="bufB"
                     /locus_tag="AC16Y7_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MNIO family bufferin maturase"
                     /protein_id="HIR5708307.1"
                     /translation="MATSAAMLEQSFGQSPIPPRAGVGLKPEHYRDILDDAPDIGWFE
                     IHAENYMGEGGTPHACLTAVRERYPLSLHGVGLSIGGASPLDTEHLARLRVLIDRYQP
                     ALFSEHLAWSSHDTVYLNDLLPLPYTAETLDRVCGHIDEVQDTLQRQMLLENPSTYVA
                     FAETSMSEVAFLCEIVRRTGCGLLLDVNNVFVQATNHGFDARTYLDSFPVEHVREIHL
                     GGHAEDEDDDGASLLIDDHGREVADPVWGLYAHALSRTGPVPTLIEWDNDVPSWPALF
                     AEAKLADAAIVERRSNRAAV"
     gene            13607..14380
                     /locus_tag="AC16Y7_02635"
     CDS             13607..14380
                     /locus_tag="AC16Y7_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HvfC/BufC N-terminal domain-containing protein"
                     /protein_id="HIR5708308.1"
                     /translation="MARRADETEAVFARAVLDRNAAVPAGLAPREGTTPSRRFDVYRN
                     NVYASLIDVLAARFQVTAQLVGETFFRAMARDYVQAEPPRSPVLLRYGAGFAGFIAGF
                     PPADPVPYLADVARLEWAWHKAYNAADARGLPLEALQANAGDVECATLCLHPSLGVVR
                     SVYPVVTIWQLAMRDGEDEPARLPADGEDALVARPELTVDVRRLPEGGADFVLALQGG
                     ANLQSAAAVATVSAPDFDLKANLAGLMSSGVIVGIGGDA"
     gene            complement(14590..>14876)
                     /locus_tag="AC16Y7_02640"
     CDS             complement(14590..>14876)
                     /locus_tag="AC16Y7_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HIR5708309.1"
                     /translation="DLDIEVKENTLAVRGEKTVEQENSTVLHRGIAARSFERRFQLAD
                     YVVVKGAALENGLLHIDLERELPEAMKPRTIEIATKTTEQPKVLDSQAAA"
BASE COUNT         2596 a         4696 c         4782 g         2802 t
ORIGIN      
        1 gaccacgacg ctcgtggatt ctcttggggc catcgaccgc ttttatgagc tatctctaca
       61 gcaggcgatt tggcgctatc tcgtcctcgg cacctttgtc agctacgaaa ttgccgacta
      121 cgctgccacg cctcaggtcg accagcgctt cggcaccggc gcatcggcgg agtactactt
      181 caatccgtat atgtccgtat acacggagta tgactacagg gacttcttct ccaacaatga
      241 gtttgatgac tttacggaga accgcgtgcg gatcggcatg cgcattcggc gctagatcag
      301 cttcttgatt tcgacggcaa ccgcatcgcg caaatcgggc cgttccagcg caaaggccac
      361 gttggcggca agaaagccaa ccttcgagcc gcagtcgaag gcgcggcctt catactccac
      421 cgcatagaag gactggctct tgagcagcgc cagcatggag tcggtcagct ggatctcgcc
      481 gccggcgcct ttctcctgct cgccgaggag tccgaaaatc tccggctgaa gaacatagcg
      541 gccgagaatg gtcaggttcg acggtgcggt gcccggcgcg ggcttctcca ccatgccagt
      601 cacgggaaag acgtttcggt cggcgtcgcc gaccgccacc acgccatagc ttttcaactc
      661 gtcgtcgggc acgggctcca cggcgagaat attgccgcca tgcttgtcgt aaacgtcgag
      721 catttgcgcc aggcatccct tgccgggcga ctgcaccaag acgtcgggta aaaccacggc
      781 gaagggctcg tcaccgacaa tgtcacgagc gcaccaaacg gcgtggccta agcccagcgg
      841 cgcttgctgg cggacaaagc tggcgctgcc ggcctcgggc agggcctcgc tcaggagttt
      901 tagctcggcc gtcttgcccc gcgcgcgaag cgtcccctcc agttcgggct gcatgtcgaa
      961 gtggtcttcg atgacgccct tggtccggcc ggggacgaag atgacaggct cgatgccggc
     1021 cgcgcaggcc tcatcgacca cgtgctggat cagcggccgg tcgaccactg tcagcatttc
     1081 cttgggcatc gccttggtgg cgggaagaaa gcgcgtgccg agccccgcga cgggaaacac
     1141 ggccttgcgg acgcgagcgg tcattcactc ctcgttggac tgatccgtaa ccagcgggtt
     1201 ccattcccct tgaggaccta gaagtccccc aacagctttg cgatagcaag agcgaattgc
     1261 aggttatgca actggggacg gtgaccgggg gctggctata ccgcgagagc ctttttgcct
     1321 ttgcagcgtc aaaaccaccc gtcacaatcg gcgccctaac gcgaatcggt cgggaacgca
     1381 catgtgggaa tcttcgctta agagggtaag ggccgcgctt gccttatccg cgcttgcctc
     1441 cttgtctgcg ctcaccttgg ccgcgctggt cctgttgccg gtgcccacaa aagctgatgc
     1501 tgtctccgac tatcggcgcg cggtcgacgc gcgctttggc caatggctgc aagatctttg
     1561 gccggaggcc gaagcggcgg gcgtctcgcg cgcgaccttc gatcgtgaga taaaaggtct
     1621 caagctcgat tggtcgttgc cgcagatcga gccgccggat ccggcagttc ccggcggtcc
     1681 cgcgctgcct gcggccatga agccgaaacc gaagccgcaa gcggagttcg gcgtgccgca
     1741 gaactatttc aaggcgcgca gtctgaacgg cctggcggcg gccggccgtg cccgctccaa
     1801 aaaactcgca ccgaccctca aggcaatcga ggcgcaatat ggcgtgccgg ggagcgtcgt
     1861 ccttgccatc tgggggcgtg agacaggctt cggccgtgcc gcgctgcccc acgacgcgct
     1921 gacggcgatt gccacgcaag cgttcatggg ccggcgcgcg gacgagttcc gcccgcaact
     1981 catcggcgcc ttgaagatca tcgaactcaa acatgcgacg cgcgcgcaga tgaagagttc
     2041 atgggccggc gccatggggc acgtccagtt tctgcctggt gacgttgaga attacgccgt
     2101 ggacttcgac ggcaatggtc ggcgcgatat ttggaactcg gttccggatt ccttggcgtc
     2161 ggcggccaac tcgctcaaga gccaaggttg ggacggcaat cagccttggg cctacgaggt
     2221 gacgctgccg aagaatttcg actgcacgct gcaaggtccc gaccaatcgc tgccgatccg
     2281 cgagtggatc gatcttggcg tcgagcgggt aaaggggcgg ccttttccca aagagcggct
     2341 ggaggactgg accttcctgg tcctgccagc ggggctcaag gggcctgcct ttcttgccac
     2401 aaccaacttc tcggtgctga agctctataa caactcggac gtgtacgcga tgttcgtcgg
     2461 ccacgtggcg gacatgatcg cctatggctc tccggccgcc tttgtcggca agtggaagcc
     2521 ggtggagcgg ttcacccgcg accgcatctt gaagttccag aatgtcctgg tggccgaagg
     2581 gcacgacgtg ggcaaggtgg acggcctggt ggggttcaag acccggcaga gcatcggcaa
     2641 gaaggagaag gagctcggca agccgctcac ctgctacccg agccatgccc tggtgaacca
     2701 ggtgctgaag gcggcgaatt agggaaatcc ccataaattg gcggtgatgt tgcctaggtt
     2761 tcgcccgcac ccgttgctag gttcccgcca tgcgaatcat gaacatttct aaggccttga
     2821 tgtttggggc tgtctcggcg ttcctgctgg cgccttcggg gcccgcccac gcagacaggt
     2881 tccagaactg cgtgaagggc ttctggcccg ccgccaagcg tgccggtgtc agccgggaca
     2941 cgtttcagcg cgccacggcc ggcatcaccc gcgataaaga ggtgatcgag gcggccaaat
     3001 atcagcccga gtacaaaaaa ccgatcggcg agtatgtcga ccgcgccatc tcgcccaagc
     3061 gcctcgcgac cggcaaagag aagctcatcg agtacaagcc gctgctcgat caggtcgagg
     3121 caaagtatgg cgtcgaccgg catatcgtgg ttgccatttg gggcgttgag tccaactacg
     3181 gcgacaacaa aggcgaaaag ggcgtcatcc aatctctgat gacgcttgcc tgctccggca
     3241 ccaaagccaa gttcgcgcgc gggcagatcg tctcggcgct caaagtcctg caacgcggtg
     3301 acacgacctt tgagaacatg aacggctctt gggccggcgc catggggcat acccagttca
     3361 ttccgacaac ttatacggct tatgccgtgg acttcgacgg cgatggccgc cgcgacatct
     3421 gggacaccat tcccgatgcg cttggctcga ccgccgcgta tttgcgcaag tccagatgga
     3481 agccgggcca gacgtggggc tacgaggtca agctacccaa gaagttcaat ccgaagcgcg
     3541 tcagccggaa gacctaccgg actgtggcgc agtggcaaaa ggccgggatc aaacggatca
     3601 acggaaagcc cttcccgcgt ccaggcgata aggcgcagct ctttgcgccg gagggaaaga
     3661 acgggccggc atttctgctc ctcaacaact tccgttcgct gctgcgctac aaccaagcgg
     3721 actcctatgc tcttgccgtt ggacatttgg cggaccggct cgctggctat gggccatttg
     3781 ttcaggcttg gcctacgggt gagcggcggc tgtccatgga gcagcgcatg gagttgcagc
     3841 gtctgctgat cgcgctcggc catcttgaag gcgatgtgga cggcattatc ggcagcggta
     3901 cgctggaggg cgtcaggtcc tatcagcgcg tcaagaagct gcctgtcgac ggctatccga
     3961 cacagaccat tttgaagaag ctgcgtgccg atgcgccgcc gctgccaccc gtcgcggacc
     4021 cggaacccat cggcgccgtg accccgccgc ctgccgaccc gcaatccgcc gccccgcaac
     4081 ccgttggtgc tgcgaaccag caagccgcgc ctgcgatgcc tgccccggcg caagcgcctg
     4141 ctgcgccggc tccggcggct gctgttccgg ctccggccgc tccggctgct ccggctgcgg
     4201 ctgttccggc cccgactgct gccgcagccc cggctgcggc cgcgcccgca atgccggcgc
     4261 cgccggcttc tgtcgcgcca ccgcaggcgc ctgccgtccc ggccaatcag ccggcaagtg
     4321 tcgtcgtgcc gaaaagtgcg cccgctgggc agcctgtcac catgaccctc gagcgcgcgc
     4381 cctcggcgaa ttgatgctat aggtttgctc tcgtttctgg agaggtccga ttgacgatgg
     4441 ttcttagtgc gttcggtctt cgcctgacgc gggctctcat cggagccgcc gcgttcggcc
     4501 ttttgcccgc gctcatgctg gggttttcgg gccagtccgt actggcgcag accaaccaga
     4561 ccgctcaccg cagctacatc acgccgtttc cgaacggcga tcgctaccgg atcgtcgtgg
     4621 tcggcgactc cctcggcgag ggactgtggg gtggcctata tcgtgccttc gaggccgatc
     4681 cgacattgga cttcatccag cgttcccaga agtcgacggg gttcgtgaac acccgccgct
     4741 acaactggaa cggggaaatc gacaagatta tgaaggacgg caactaccaa gttgccgtgg
     4801 cgatgttcgg cgccaatgac gcgcagccga tcaagcagga cggcaaggtg ctgaaggtcg
     4861 gcaccgaaga gtggcgtgag gcctatggcg cgcgggttga gaaattcatc aagaagctgc
     4921 gtgccgcaaa tgttgcggtg tactgggtcg gcctaccggt gatgcgcagc cccggccaga
     4981 gttccaatgc agaaaagctc aacgaggtct tccgtgagaa ggcgttcgtc aacggtgcca
     5041 agttcatcga tacgtgggaa gggttcgccg acgagcaggg ccgctacagt gcatatgggc
     5101 cggacatgtc gggcaagtcg aaacggttgc gcgccaatga tgggaagcac ttcaccatgc
     5161 gcggcaacgt caagcttgcg catttcgtcg agaaggagct gcgcaaggac atcaacttgg
     5221 ccaaggcgga gcggaatatt cctcttgcgg gcaacgagca ggaacaggcc aaggtgaaag
     5281 accgcttcgt cgcgccgaag ccggtggcgc ccaagaagac gcagaacgtt gccgagggtg
     5341 gcgaggaaga ggacgcgctg cgtgaaagcc aagtcggcga cgtcagcgtg gtgaggccca
     5401 cggcgttgcc aagtgtggca gccccagccg atcggctcac ggcgcagcag gccgcgacga
     5461 cgacggagcc tgaggtgatt acgagccagc tgcctggcgg cttcacctcc atggcgacca
     5521 tttcggccgt caccgacctc acgctggctt catcgcggcc gcaactgccg ctgaagcagc
     5581 ggccctatta ccgggttctg atccagggcg aacagctgga gccgaagtcg ggccgggcag
     5641 acgatttcgc ctggccgccg agctagggtt ctcggagtcc cggcaattag cggggcagtg
     5701 tcgtcgttcc catgagtaat ttgtcgatgg cgtgcgccgc ctggcggcct tcgcggatcg
     5761 cccagacgac gagcgactgg ccgcggcgca tgtcgccggc ggcaaagacc ttcggccgcg
     5821 aggtccggta gtccagctca ttggccttga cgttaccgcg ctggtcgaga tcgaccccga
     5881 gatcctcgat catgcccttg tgcacgggat gcaagaagcc catggcgagg aacacgagat
     5941 cggcgggaat ggtgaactcg ctgtccggaa tcggcttcat ctgatcgtcg agacgcacgc
     6001 attcgagctg ctcgaccttg ccgtcgccga gaaagcggtt ggttgcaacg ctgaaatcgc
     6061 gctccgcgcc ttcggcctga ctggacgagg tgcggaactt cagcggccag tgcggccagg
     6121 tcagcgcctt gttctccatc tccggcggct ctggcatgat ctccagctgc gtcaccgatg
     6181 tcgcgccctg acggaacgag gtgccgatgc agtcggagcc cgtgtcgccg ccgccgatga
     6241 ccacgacttg cttgccgtcg gcgacgatct cctccacgtc gcctaaaggc tcgcccgaca
     6301 cacggcggtt ctgctgcggc agaaatgtca tggcgaagtg gacgcccgga agatcgcggc
     6361 ccggcacggc caaatcgcgg ggcgcttccg acccgccggc cagcagcact gcgtcatggc
     6421 cgcgctccag ctcgccgatg gcgacgtcct tgccgacatt gacgttgcaa tggaacgtca
     6481 cgccctcggc ttgcatctgc gccacgcggc gttcgatgac gtatttctcc atcttgaagt
     6541 ccggaatgcc gtagcgcagc aggccgccag gttcggcatg cttctcgtag acatgcacgt
     6601 cgtggccggc gcgcgcgagc tgttgcgcgg cggcaaggcc cgcagggccg gagccgacaa
     6661 tggccacgcg cttgccggtg gtcttctccg gcggctgcgg cttgacccag ccttcggaga
     6721 aggcccggtc ggcaatggcg cactcgatgg atttgatggt gaccggctcg tcgatgatgt
     6781 tgagcgtgca ggcggcctcg cacggagcgg ggcagatgcg cccagtcact tccggaaagt
     6841 tgttggtcga gtgcaggttc cgcgtcgcgt ccaaccaatc gtcctgatag acgaggtcgt
     6901 tccagtccgg gatctggttg ttcaccgggc agccggtgtg acaatacgga atgccgcaat
     6961 ccatgcagcg ggcggcctgg cgcacaagct ccgcctcgcc cggcgcaatc acgaattcct
     7021 tgaaatggcg cacgcgatcg gcggccggct catagccccg ctcctgccgc tcgatctcca
     7081 aaaagcccgt gatcttgccc attaggtcac gttctccgcc ccaaaatctc aaccaaagaa
     7141 gcgcgaacag acccgcgctc ccattcttca ctcaagcgta tcgcatcttc gcgtggcagg
     7201 accaccggcc tggcgcttgg agctccaggg cgtggccgca tccctgcctg gttatttgcc
     7261 gttgccgcgc aggccgatct cgatttcgcc gaggctcgtg gcatcggcgg cctgctggcg
     7321 cgccatctcc tgcaaggctc gccggtactc gaccggcatg actttgatga atttcggacg
     7381 atacgtggcc cagttgtcga ggatctcccg cgcgcgggtc gagcccgtgt agtgcagatg
     7441 gttctcgatc agccggtgca gccgctcctc gtcatgacgc gtcatgtcgg tcaagtcgac
     7501 aaggccgtgg ctctccagat cgccgccctg gtggcgctgc tgctccatcg cctggttctc
     7561 gcccggaatc ggctcgaggt cgaccatggc catattgcag cgctgctcga agtcgccgct
     7621 ctcgtcgaga acgtaagcaa tgccgccgga catgccggcc gcgaagttgc ggcccgtcgg
     7681 gccgatcacc acgaccacgc cgcccgtcat gtattcgcag ccatgatcgc ccgtgccttc
     7741 gaccacggcg atggcgccag agttgcgcac gccaaagcgc tcgccgccga cgccgcggaa
     7801 atagcactca ccggcgatgg cgccatacag caccgtgttg ccgacgatga tcgatttctc
     7861 cggatcgatg ccggactcct ccggcggccg gacgatgatg cgcccgcccg acaacccctt
     7921 gccgacatag tcgtttgcct cgccgacgag atcgagggtc acgccattgg ccagccaggc
     7981 gccgaaggct tgcccggcgg agcccttgaa gcggatccag atcgtgtcgt ccgacagccc
     8041 cgcatggccg tagcgcttgg cgacctcgcc ggacagcatg gcgcccgtgg agcggtcgac
     8101 attcttgatc ggcaggtcga gcttgaccgg cttcttctct tcgagagcat cgcggcaaag
     8161 ctcgatgagc ttcatgtcga ggacgctctc caagccatgg tcctgacgct cggtgtttct
     8221 gatcggcaaa gaggcggcca cgtcgggctt gtggaacagc ttggtgaagt cgagaccgcg
     8281 tgccttccag tggtcgatcg ccttctcctt gtcgagcact tcgctgcggc cgatcatttc
     8341 atcgaagctg ccgaagccga gcgcggccat gtactcgcga acctcttcgg cgacatagaa
     8401 gaagtaattg atcacgtgtt cgggcgtgcc cgtgaagcgc ttgcgcagca cggggtcttg
     8461 cgtcgcgatg ccgaccgggc aggtgttcag atgacacttg cgcatcatga tgcagcccgc
     8521 cgcgatcaga ggcgcggtgg agaagccgaa ctcgtcggcg ccgagcagtg cgcccaccac
     8581 cacgtcgcgg cccgtgcgca agccgccgtc cacttgcacg gcgatacgcc cgcgcaggcg
     8641 gttcagcacc agcgtctgct gagtttcggc caagccgatt tcccaagggc tgcctgcatg
     8701 tttgatcgag gtgaggggcg atgcgcccgt gccgccttcg aaaccggcaa tcgtcacatg
     8761 gtccgccatg gccttggcca cgcccgcggc aaccgtgccg acgccgacct cggagacaag
     8821 tttgaccgag acgtcgcctt gagggttcac gttcttcagg tcgaagatga gctgcttaag
     8881 atcttcgatc gaatagatgt cgtggtgcgg tggcggcgag atcagcccca cgcccggcgt
     8941 cgagtggcgc accttggcga tggtggcgtc caccttgtga ccaggcagct ggccgccttc
     9001 gccgggcttg gcgccttgcg ccatcttgat ctgcatcatg tcggcattga cgagatactc
     9061 ggtcgtcacg ccgaaacggc ccgatgccac ttgcttgatg gccgagcgca tggagtcgcc
     9121 gttctccatg ggcgtgaagc ggtccacttc ctcgccgcct tcgccggtgt ttgaccggcc
     9181 gccgatgcga ttcatggcga tggccagcgt ggtgtgcgcc tcgcggctga ttgaaccgaa
     9241 ggacatggcg ccggtggcga agcgcttgac gatctcggaa acaggctcga cctcgtcgag
     9301 cgggatcggc gttcgcccga tctcgtcggc gttcttgatg cggaacaggc cgcgcagggt
     9361 cagcagctgc tgcgattgtt cgttcacggc ttccgcgtat tcgcggtatt tctccggaag
     9421 ctcgccacgc actgcgtgct gcaggtcggc gaccacctcg ggacgccagg tgtgcgcttc
     9481 gccgcggaca cggaaggcat actcgccgcc aacgtcgaga gcattcttca agacgggcgc
     9541 gtcgctgaac gcgagcttgt gccgctccac ggtctccccc gcgatctctt cgagtccgac
     9601 gccctcgatc tgcgtgttgg tgccggtgaa atacttggcg acgaagtccg agtgcaggcc
     9661 taccgcgtcg aagatctgcg cgccgcaata ggactggtag gtcgagatgc ccatcttgga
     9721 catgaccttc aaggtgccct tgtccacggc cttcatgtag cgcgtgatgg cttcttcgac
     9781 cgtcaggtct tcgtcgagtt ccggcacgat ggcttcgatg gtctcgaacg ccagataagg
     9841 attgatcgcc tcggcgccat aaccggcgag cgtgcagaac tggttgacct catgggcctc
     9901 gcccgtctcg acgacgagac caacggaggt cctgagacct tcgcggatca agtgatggtg
     9961 cacggcgctg gtggccagca gcgaggggat cggaatgcga tcgggccccg tcgcgcgatc
    10021 cgacaggatg ataatgttgt cgccctcgcg gacggctttc tcggcttctt cgcacagcgt
    10081 ctcgagcgca ccggccatgc cgtcggcgcc gtcctcgacc ggataggtca tgtcgagcgt
    10141 gacggtcagg aactgattgt cggcgaccgc gccgatggcg cggatgcgtt ccaggttctc
    10201 gttggtgaga atgggatggg ccgcctccag gcgcttgtcc ttcaaggtgc cttccaggtt
    10261 gagaaggttc ggcctcggcc cgatgaaggt caccagcgac atgacgagct cctcgcggat
    10321 cggatcgatc ggcgggttcg tcacttgcgc gaagttctgc ttgaaatagg tgtgcagcag
    10381 cttgggccgg tcggacaaag gcgcgatcgg cgtgtccgtg cccatggagc cgatgggttc
    10441 ctgacctgtc tggatcatgg gaatcatcat gaacttggcg ctctcctgcg tgtagccgaa
    10501 ggcctgttgg cgatcgagca ggctcacatt ggtgcgcgcc ggcggtgcag ggctgcccgg
    10561 cagctcgcgc acgtggatct gcgtgcggtc gagccattgc tgatagggat tggctccggc
    10621 cagctccgcc ttgatctcct cgtcggagat gatgcggccc ttctccaagt ctatgagcag
    10681 catcttgccg ggctgaagcc gccatttctc gacgatcttc tcttcggcga aaggcagcac
    10741 gccggtctcc gaggccatca ccacaagacc gtcgtcggtc acgaagtagc gcgccggccg
    10801 caagcccttg cggtcgagcg tggcgccgat ctgacggccg tcggtgaagg ccatggcggc
    10861 agggccgtcc cacggctcca tgaggcaggc gtgatactcg tagaaggcgc ggcgcttggc
    10921 atccattagc gggttgccgg cccaggcctc cgggatcagc atcatcgcgg catgggccag
    10981 gctgtagccg ccttgcacga ggaactcgag cgcgttgtcg aagcacgccg tgtccgactg
    11041 gccttcgtag gagatcggcc acagcttcga gatgtcctcg ccgaacagtg gcgacgacac
    11101 gctcgcctgg cgcgcggcca tccagttgac gttgccgcgc agcgtgttga tctcgccgtt
    11161 atgggcgacc atccgatagg gatgcgacag cttccaggac gggaaagtgt tggtcgagaa
    11221 gcgctgatgc acgagagcga gcgcagaggc gaatttcggg tgatgcagat ccgggtaata
    11281 ggcgccgagc tggtaggcca ggaacatgcc cttgtagacc agggtgcggc aggacagcga
    11341 tacgatgtag aagccgatgt cgcgtccgcc ggtctcgccg ttgatcgtgt tggagatcac
    11401 cttgcgcagc agatagagcc ggcgctcgaa ctccgtctcg tcctcgatgc cggggccgcg
    11461 cccgataaag acctgccggt gcataggctc ggtctcgacg acttccggcg acagcgtggc
    11521 gttgtccgtc ggtacggtgc gccagccgag aagctgcaaa ccttccgcct cgatcacgcg
    11581 ctcgatggcg gactcgcaat agatgcgctg cgcctggttg gacggcatga agatctgccc
    11641 aaccgcgtaa cggcccggtt ccggcagctt gatgttcagc ggtgcgcaga cctcctcgaa
    11701 gaaggcgtgc ggaatctgca ccagcatgcc ggcgccatcg ccgtcgcggg ggtccgcgcc
    11761 ggccgcgcca cgatgggtca ggttctccag gatctggagt ccctgctcga tgattttgtg
    11821 gctcttgcgc cccttcatgt cggcgatgaa gccgacgccg caggcatcgt gctcgttggc
    11881 cggatcgtag agtccttgcg ctcgcggcag tcctttcgga gccacgtcgg tcaatccagg
    11941 aatgagcgca cggggtgcca ttgtgagtat ggaacctcgg cctcgcccca agtcgggagc
    12001 gggcgtctag agtttggcga acggccgtcc tcttcaccag aagaacgtgt gaggtagaag
    12061 agaaggtccg gcccttggag gctagcgcct ttgcgccagc gaatgcgggc tttagagacc
    12121 gtattccgcc cctaatgaag cgtctttaaa gcctcgcgcc gcagcccccg gaacaaaggt
    12181 cagcatgcct gtcctattta gtgtcaaaat gacagatttc caaggggtgc acaagcacga
    12241 ttcggtgacc ccaagggccc aaaggggcgc acgcctgttc acctcgttct agcggaggcg
    12301 tgatttgacg tgagcgcgcc cgcgctggcc tactcttggg gtgattgggc cgctggcccc
    12361 cggaaaactg cctttttttc tgaccccaac gaggatttta catgaccgat caatccaaga
    12421 cgatcacggg cctggccctg gcttccgcct tcgccgctgc cctcggcctt gccgccacca
    12481 gcgccgccgc cgaggacaag aagaaacagg tggagtgcta cggtgtcgcc aaggccggtg
    12541 ataacgactg cgcggccggc ggggatacga cctgcgccgg gagctctacg gtggattatc
    12601 aaggcaatgc ctggaagctc gtgcctgagg gcacctgcac gaccatggaa ctgccggatg
    12661 gccgcaaagg gagcctgaag ccgctcgacc gcgatctgcc gccagtttaa gcggtcatgg
    12721 cgacctccgc tgccatgctg gagcaatcct tcggccaaag cccgatcccg ccccgcgcgg
    12781 gcgtcgggct gaagccggag cactatcgcg acatcctcga tgacgcgccc gatatcggct
    12841 ggttcgagat ccatgccgag aactatatgg gcgagggcgg gacgccccat gcctgtctca
    12901 cggcggtgcg cgagcgctat ccgctgtcgc ttcacggcgt ggggctttcg atcggggggg
    12961 cgagcccgct cgatacagaa caccttgccc gtttgcgcgt cctgatcgac cgctatcagc
    13021 cggcgctgtt ctccgagcat ctggcctggt cgagccacga caccgtctat ctcaacgacc
    13081 tcttgccgct gccctatacg gcggagaccc tcgatcgggt gtgcggccat atcgacgagg
    13141 tgcaggacac gcttcaaagg cagatgctgc tggagaaccc gtccacctat gtcgccttcg
    13201 ccgagacctc catgagcgag gtggcgttct tgtgcgagat cgtgcggcgc accgggtgcg
    13261 ggctgctgct cgacgtcaac aatgtcttcg tgcaggccac caatcacggt ttcgacgcgc
    13321 gcacctatct cgacagcttt cccgtcgagc atgtccgtga gattcatctc ggcggccatg
    13381 cggaagatga ggacgacgac ggcgcctcgc tcctcatcga cgaccatggg cgcgaagtcg
    13441 ccgaccccgt ctgggggctc tacgcgcatg cgctgagccg caccggccct gttcccacgc
    13501 tgatcgaatg ggacaacgac gtcccgtctt ggccggcgct ttttgccgaa gccaagctgg
    13561 ccgacgctgc gattgttgag cggcgtagca accgcgcggc ggtgtgatgg cgcggcgcgc
    13621 ggacgagacc gaagccgtgt ttgcccgcgc ggttctggat cgtaacgccg ccgtgccggc
    13681 gggtctcgca ccacgcgaag gaaccacgcc gtcgcggcgc ttcgacgttt accggaacaa
    13741 tgtttatgcg agcctgatcg atgtgctggc cgcgcgcttt caagtcacgg cgcagcttgt
    13801 gggcgagacg ttcttccgcg ccatggcgcg agactatgtg caagccgaac cgccgcgttc
    13861 gccggtcctg ctgcgttacg gcgcgggttt tgccggcttc atcgctggct ttccgcccgc
    13921 ggaccccgtg ccttatctgg ccgacgtggc ccggctggaa tgggcgtggc acaaggccta
    13981 taacgcggcg gacgccagag ggttgccgct tgaggcgctg caagcgaacg ccggtgacgt
    14041 ggagtgcgca acgctgtgcc tccatccgtc gcttggcgtc gtgcggtctg tttatccagt
    14101 ggtgacgatc tggcagcttg cgatgcgcga tggcgaagac gaaccggcgc gccttccggc
    14161 cgacggcgag gatgcgctgg tggcgcggcc cgagcttacc gtcgacgtgc ggcgcttgcc
    14221 ggaaggcggt gcggacttcg tgctcgcttt gcagggcggc gccaacttgc agagtgctgc
    14281 ggccgtcgcc accgtttctg cgccggactt cgacttgaaa gcaaatctcg cagggctgat
    14341 gtcgagcggc gtgatcgtcg gcattggcgg cgatgcttaa cgaaaccttt ccccaatagg
    14401 tgagctgtta agccccgcga aagttgtaag gcgtacgctt tctactgccg ctgattgtgt
    14461 ccagcgttct gcggcactcc tggcaggttg ttctctccca tagccacctg cggaatcgag
    14521 aagggcgccc gccgatcgac ggaggacgcc cttcttgttt gcgaagaccg cttttcacca
    14581 gatgatggct tacgcggcag cctggctgtc gagcaccttg ggctgctcgg tggtttttgt
    14641 ggcgatctcg atggtccggg gcttcatggc ctcgggaagc tcacgctcga gatcgatgtg
    14701 cagcaagccg ttctcaagcg ccgcaccctt caccacgaca tagtcggcga gctggaagcg
    14761 ccgctcgaaa ctgcgcgccg cgatgccgcg atgcaggacc gtggagttct cctgctcgac
    14821 ggttttctcg ccgcgaacgg caagcgtgtt ctccttcacc tcgatgtcga ggtcgc
//
LOCUS       DBNHVU010000070        12011 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1290719, whole genome shotgun sequence.
ACCESSION   DBNHVU010000070 DBNHVU010000000
VERSION     DBNHVU010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 12011)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12011)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12011
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1290719"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="AC16Y7_02645"
     CDS             complement(<1..147)
                     /locus_tag="AC16Y7_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR5708310.1"
                     /translation="MQKSLNVPSAAEALPGRPDPIPTAQTHFVTGAPLKGPYPEGAET
                     ALFGL"
     gene            complement(222..539)
                     /locus_tag="AC16Y7_02650"
     CDS             complement(222..539)
                     /locus_tag="AC16Y7_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708311.1"
                     /translation="MRFVLRILGVWLLLLAMVAAVVDATKSLAGGGAWVVTPMGDQWR
                     QLAPESLQATRQWIIANAGDWLWDPIMVSILAAPTWVVFGILGVFLYWLGQKRKEVEV
                     FIN"
     gene            679..768
                     /locus_tag="AC16Y7_02655"
     tRNA            679..768
                     /locus_tag="AC16Y7_02655"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:713..715,aa:Ser,seq:gga)
     gene            complement(834..1217)
                     /locus_tag="AC16Y7_02660"
     CDS             complement(834..1217)
                     /locus_tag="AC16Y7_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708312.1"
                     /translation="MTMTHKVLKVAAASAILALTAANIPARADDSVQNLGPVGPSEPI
                     LATFGNKQLVASFATGDGACDMQAVVWNADDIDARSASRLHVRVSSGQRAEIDGSTNE
                     SITLHCGESAQTLAVVTADMEVAAK"
     gene            1765..2490
                     /locus_tag="AC16Y7_02665"
     CDS             1765..2490
                     /locus_tag="AC16Y7_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR5708313.1"
                     /translation="MITLYAFGSAFGLPDVSPFVTKVETLLKMADLEFAVDTSGFNKA
                     PKGKLPYIDDDGERIADSTFIGWHLEKKYGVDFDKGLDDEQRAVAWAFEKMLEDHTYW
                     TVLHSRWVDDANFAAGMAQFFERIPLPMRLIVPRMARRQIKAELRGQGMGRHAQEEIV
                     ALGKQSLDAAAAFLAGKPYMMGDEPTGLDATAFAFFAGALCPLFETPLREAAERHANL
                     KAYVGHMAGRYYPDHTEIARWAA"
     gene            2653..3636
                     /locus_tag="AC16Y7_02670"
     CDS             2653..3636
                     /locus_tag="AC16Y7_02670"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR5708314.1"
                     /translation="MTDEAVLSLLEKRLGRRHARQRLGVEKDHEAQVFGQGINFFHIE
                     NLTIGHAVIRAVLMGTGLYWRGLRNAAKVEVRENLIDAPHLPEAFDGLSILQISDLHV
                     DMSEGAMARLIQLLPTIPYDLCILTGDYRGNTWGPYEPSLAGMEKLCAQLKGPVYGVL
                     GNHDTILMVPRLEEMGVRMLLNEADTIERGDERLHLAGVDDAHFYRMENIEKAADNIP
                     HEEFSILVTHTPEIYRQAAHADFDVLISGHTHGGQICLPGGIPITLSAVLPRALGSGP
                     WRHHDMIGYTAVGAGSSVVPVRFNCPPEVTLHRLRRRRETTVSKAPRGGSA"
     gene            complement(3605..4093)
                     /locus_tag="AC16Y7_02675"
     CDS             complement(3605..4093)
                     /locus_tag="AC16Y7_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HIR5708315.1"
                     /translation="MQPLLIIQLLVLIAVANAAPLFLKKAMGTALDWPLDGGLTLTDG
                     HPLFGVSKTIRGIVAALLTTPAVSALMGLGWELGALVAACAMAGDLLSSFIKRRFGLA
                     PSSQAIGLDQIPESLLPFIAASWLVPIGVLDIVAGTAIFAVGELAVSRILFRLNLREE
                     PY"
     gene            4203..5453
                     /locus_tag="AC16Y7_02680"
     CDS             4203..5453
                     /locus_tag="AC16Y7_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008126355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5708316.1"
                     /translation="MARQTGHTAQEGAAGQRTAVAVLAAISVCHMLNDVIQSLILAIY
                     PMLKTSLALDFAQIGFITFTFQATASVLQPLVGYVTDRYPTPYSLVVGMASSLAGLIL
                     IAFASSYAMVLGAAALIGTGSSVFHPESSRVARLASGGRHGTAQAVFQVGGNFGTALG
                     PLLAAFIVIPYGQGSIAWFSVVALLGMALLSVVGNWYGKALKWKPVSHPEEREAIAAL
                     GRREVARAIAILVTLTFSKHFYLASIASFYIFYLVQTFDLTVQSAQLFLFLFLGAVAA
                     GTVIGGPIGDRIGTRRVIWWSIVGVLPFTLALPYVGLVGTAILSVVIGLILASAHPAI
                     IVYAQSLLPGRVGMVAGLFFGLAFGIAGIGAALLGRLADATSITFVYHVCAFLPAIGL
                     LAAFLPEMSKAKPRKRQKKRPATR"
     gene            5800..6660
                     /locus_tag="AC16Y7_02685"
     CDS             5800..6660
                     /locus_tag="AC16Y7_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708317.1"
                     /translation="MRDIRGDLQDRASFLKEQLSAAQAQFEKRQEQLKREHEARIEDL
                     QSEFEAVTTLMELEYQRLTDAPVQDEPRQAPPVAERHPVREAEPDRRPAPEAEYQRRQ
                     EQEPYRQANGRATEPQRRPSREPAQYRAPHAAEAVPQRRPGPVPVRQEQVQERVHERV
                     QERAHEDEGEQRSAAPQIYRRAPLAAAPAREGEHEQPRPAERVSQAPQQPPLADFLIR
                     KLSELGAMTFEDLCHIAVQEGYFANGDVDRGVHVTLTNVAKAGFIRQQPDGTFSPATV
                     MDTIRLRRAI"
     gene            complement(6682..7476)
                     /gene="olsA"
                     /locus_tag="AC16Y7_02690"
     CDS             complement(6682..7476)
                     /gene="olsA"
                     /locus_tag="AC16Y7_02690"
                     /EC_number="2.3.1.270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lyso-ornithine lipid O-acyltransferase"
                     /protein_id="HIR5708318.1"
                     /translation="MKRVRAFLVLATFFAFTLPLMPLQYLLVRTGSRFARSFPHWYHR
                     QVCRIIGIRLNVDGEVAESQGVLIVSNHVSWLDITVLSAVAPVSFVAKQEVSTWPFVK
                     WLAKLQRSVFVDRERRTEVGTKANEILERLEAGDHVVFFAEGTSSDGNSVVPFRSALF
                     AAVKPPGGAPMGSKVSVQTLALTYTKLHGLPLGRRGRPQVAWYGDMDMASHAWKLLGL
                     GPLDADIRIGPPVPLDDFPDRKALARYTETKVRNDVVELLRARPSR"
     gene            complement(7513..7923)
                     /locus_tag="AC16Y7_02695"
     CDS             complement(7513..7923)
                     /locus_tag="AC16Y7_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708319.1"
                     /translation="MGRRIVLAAIVTLLSGMAFAGHALEPEAAPVAKEDNSVETDNAA
                     ASVTEVTASPAQPVSTPSPTTIVVPAPKVEVKMPSELDLYIVPAATREVCTTRDWGAG
                     EIETDCRVRPVPIMRADPQLRGLCVTRYGQRVCY"
     gene            complement(8020..8454)
                     /locus_tag="AC16Y7_02700"
     CDS             complement(8020..8454)
                     /locus_tag="AC16Y7_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR5708320.1"
                     /translation="MSGEATRESRLERLCADRGLRMTGQRRVIARVLSEAHDHPDVEE
                     VYRRASAEDPRISLSTVYRTVRLMEGEGIVERHDFGDGRARYEPAGHDHHDHLINMKT
                     GEVIEFKNEDIERLQERVAKELGFRLIGHRLELFGTPLKNGK"
     gene            complement(8582..9040)
                     /locus_tag="AC16Y7_02705"
     CDS             complement(8582..9040)
                     /locus_tag="AC16Y7_02705"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5708321.1"
                     /translation="MTRTCAVSRAGPGDAALLAALHARCFAKPWDEIAMARFVAAPGV
                     LCLVGTAPHEVAAPAALLIARAAADEAELLTMGVIPERRRAGLGRALLSHALAVLSSR
                     GATQLFLEVDETNAAAVALYRASGAKPAGRRERYYESGANAAIFSLDLRS"
     gene            complement(9040..9726)
                     /gene="tsaB"
                     /locus_tag="AC16Y7_02710"
     CDS             complement(9040..9726)
                     /gene="tsaB"
                     /locus_tag="AC16Y7_02710"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR5708322.1"
                     /translation="MNILALDTSMGACSAAVLRAGDARRLEALQVEMPRGHAEALMPM
                     VDEVLAEAEIGASELDLIAATVGPGSFTGMRIAIAAARGLALVTSAKLFGTDSLSVMA
                     RAALLAGADPQGPFAVAVDARRGMLYLGLFDETAAKMDGPLLIAPDDAAAMLPKNLAL
                     AVGSGAGALAESASARGLSVGATLETLQPSAAALAEIAHERGETCEVLRPLYLRPPDA
                     KPQAPTLERR"
     gene            complement(9736..10326)
                     /locus_tag="AC16Y7_02715"
     CDS             complement(9736..10326)
                     /locus_tag="AC16Y7_02715"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HIR5708323.1"
                     /translation="MTAKLPDASLDQLFRDARSRGAWQDKDVPDSLLHELVDLVKLGP
                     TSGNCSPARFVFVKSKNAKERLEPYLSAGNREKTMKAPVCAIIGYDLDFYEHLPTLFP
                     HTDAKAWFEGKPQKIADSAFRNGTLQAAYLILAARGLGLDCGPMSGFDQEGVDLAFFA
                     GTNVKSNFLCNLGYGNDAPLRPRAPRFDFDQMAQIV"
     gene            complement(10323..10883)
                     /locus_tag="AC16Y7_02720"
     CDS             complement(10323..10883)
                     /locus_tag="AC16Y7_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HIR5708324.1"
                     /translation="MFIQTEATPNPATLKFLPGRAVLGEADAIEFRDQTESEGSPLAA
                     RLFDIDGVTGVFLGSDFISVTKGDGEWQHMKPAILGAIMEHYLSGEAMTEGDPAQYDG
                     VNESYAPEDEETVKTIKELLDTRVRPAVAQDGGDITFQGYRDGVVYLHMRGACAGCPS
                     STATLRHGIENLLRHFIPEVQEVRPT"
     gene            complement(11028..11687)
                     /locus_tag="AC16Y7_02725"
     CDS             complement(11028..11687)
                     /locus_tag="AC16Y7_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5708325.1"
                     /translation="MATPKKKPAKKTPPAKQKSAPRKAAPKKAAPKNAAPKKPAPAAK
                     AASSTPRARKKREIYLEGHKRKFLVAVDETPECDRALAFAASRAKRTKGQLALLYVIE
                     PEGEALHWLGVEDMAREEGLSKAKAVFRLFGRKLKALGFEALVPEEIVREGSKAEEIT
                     KAIEEDQDIGVLVLGASRDPSGPGPLVTHLAGGKLAGSFPVPITVVPGHLTTEEILAL
                     A"
     gene            complement(11777..>12011)
                     /locus_tag="AC16Y7_02730"
     CDS             complement(11777..>12011)
                     /locus_tag="AC16Y7_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708326.1"
                     /translation="KKKPYPLRDLPKDCRTVLTAGDYELLSVDDELPPVALKAAASKE
                     AGPGVPLFTKTQLHNVLKGGQVNVPMPNRNPVR"
BASE COUNT         2173 a         3785 c         3776 g         2277 t
ORIGIN      
        1 aagcccaaac agggccgtct cggcgccttc gggatacggg cccttgaggg gggcgccggt
       61 gacaaaatgg gtctgcgccg tggggatggg atcgggccgg ccgggcaagg cctctgcggc
      121 gctgggaacg ttgagggact tttgcatgag ggctctccgt atagcttcaa acataggagc
      181 tccctcgacg ggtttcaggc ggcccgtcct tcacattccc gctagttgat aaagacctcg
      241 acctcttttc gcttctggcc gagccaatac agaaacacgc cgagaatgcc gaataccacc
      301 caggtcggtg cggccaggat cgagaccatg atcggatccc acagccagtc tccggcattg
      361 gcgatgatcc attgccgcgt tgcctgcagc gattctggcg cgagctggcg ccattgatcg
      421 cccatgggcg tcaccaccca cgcgccgccg cccgccaggc tttttgtggc gtcaacgacg
      481 gcggcaacca tggccagcaa aagcagccag acaccgagta ttcgaaggac aaaacgcatg
      541 ggcgagagtg gccgatcccg tgtttccgct tacataagcc attggccggt cccgttcaaa
      601 cgcttcgagg ccgcgtcctg gcgtcctggc cgctccgctt gaacggaagg tgcgcttcgc
      661 tatagtgcgg cgcctttagg agaggtggcc gagtggttga aggcgcacgc ctggaacgcg
      721 tgtaggcggg taaccgtctc gagggttcga atccctctct ctccgccaaa aagtgagagg
      781 tcccagactc agcaacatcc gggacctcta tacctctcta caatttagct cactcatttg
      841 gcggcgactt ccatatctgc agtcacaact gcgagggttt gtgcggactc accgcaatgg
      901 agcgtaatcg actcgttcgt cgagccgtcg atctcagcgc gttgacctga gctgacgcgc
      961 acatgaagtc gcgatgcgga tcttgcatcg atatcgtccg cattccatac caccgcctgc
     1021 atgtcgcaag caccatcgcc agtagcaaac gacgcgacga gctgcttatt gccgaaggtt
     1081 gcgagaattg gttcgctcgg tccgaccggc cccaaattct ggacactgtc gtcggcacgg
     1141 gcgggaatgt tagcagcggt aagagcgagg attgcgcttg cagctgccac tttcagtacc
     1201 ttgtgggtca tagtcatcgt ggtgtctcct tcgcttggct gctcaagcct ttcttgagca
     1261 tggaaggaat tctagacccg gcctccgttc gacgctgtgt caattgccac actgccgaat
     1321 acggtgatca ccaaccctgc tcggcagttg tctgaggctc tgggtcatta gctgacattt
     1381 ttagtccagt atgagaaatg gccactttgc cccaacagcg gacttgctca gcaagcattg
     1441 ggacaggact atttgcaaac ggaatggcaa acgaaccgca tggcacagca cggcataggc
     1501 tgtcacaaca gcgcattttt ggttaaagaa agcccatcag aagatgaagt ttagctactg
     1561 cgcggtacag atcggcacga gagtcttaga actcgaaaac cgttgagcgc gcaagcgttc
     1621 cgagggttcg aatccctctc tctccgccag gcacttgtgc tttcggcgct ccgctcgcca
     1681 agaagcgcct gccccgccgc cacgatcctg acaccgccag cttggacaat cggcgcctgg
     1741 tcttgatcgc ccatgggagc accgatgatc accctttacg cctttggatc cgcctttggg
     1801 cttcctgacg tcagcccctt cgtcaccaag gtcgaaacgc tgctgaagat ggcggatctc
     1861 gaattcgcgg tcgacacgag cggcttcaac aaagccccga aaggcaagct tccttacatc
     1921 gacgacgacg gcgaaagaat cgccgactcc accttcattg gttggcactt ggaaaagaaa
     1981 tacggggtcg acttcgacaa ggggctcgat gacgagcagc gggctgtcgc ctgggccttc
     2041 gagaagatgc ttgaggacca cacctattgg accgtcttgc actcacgctg ggtcgacgat
     2101 gcaaacttcg cggccgggat ggcgcaattc ttcgaacgca tccctttgcc gatgcggctc
     2161 attgttccac gtatggcgcg ccggcagatc aaggccgagc tgcggggcca aggcatgggg
     2221 cgtcacgcgc aagaagagat tgtcgcgctc gggaaacagt cgctcgatgc cgcggccgcg
     2281 tttcttgccg gcaagccata tatgatggga gacgagccga ccgggctcga cgccacggca
     2341 ttcgcatttt ttgccggcgc cctctgtccg ctgttcgaga cacctttgcg cgaagccgcg
     2401 gagcggcacg ccaatctcaa ggcttatgtc ggccatatgg cgggacgcta ctaccctgat
     2461 catacggaga tcgcccgctg ggccgcttga cgtcgagcgc ctcgtaaatc tttgcgatgg
     2521 tctcgtagcg cacgccgggc attttccccg tccgcagccg cgacaggttg gatatccttg
     2581 gttgtcgaaa tgaccccttg cccttgagct ttggtttggc aagtctgctg caatggagcg
     2641 cacccgggat cgatgaccga cgaagccgtt ctttcactcc tggagaagcg tcttggtcgg
     2701 cgccatgccc gccagcgtct cggcgtcgag aaggatcacg aggcgcaggt cttcggccag
     2761 ggcatcaact tcttccacat cgagaatctg acgataggcc atgccgtcat ccgcgcggtt
     2821 ctcatgggca cggggctcta ttggcggggc ttgcgcaacg cggcgaaagt tgaagtccgc
     2881 gaaaacctca tcgacgcgcc ccatctgccg gaagccttcg acggcttatc cattctccag
     2941 atcagcgatc ttcacgtcga catgagcgaa ggcgccatgg cacgcctgat acagctcctc
     3001 ccgaccatcc cctatgatct gtgcattctg acgggcgact accgcggcaa cacctggggg
     3061 ccctacgagc cgtcgcttgc gggtatggag aagctctgcg cccagttgaa ggggccggtc
     3121 tatggtgtgc tcggcaatca cgacacaatt ctcatggtgc cgcgattgga ggagatgggc
     3181 gtgaggatgc tgctcaacga ggccgacact attgagcgcg gcgacgaacg cctccatctc
     3241 gcgggtgtcg acgatgcgca cttctatcgc atggagaata ttgagaaggc tgccgacaac
     3301 attccccatg aggagttctc gatcctcgtc acgcacacac ccgagatcta tcgtcaggcc
     3361 gcccacgccg atttcgatgt gctgatcagc ggccatactc atggcgggca gatctgcctt
     3421 cccggcggca ttcccatcac cctaagcgcc gtgctgccgc gcgcgctcgg ttctggacct
     3481 tggcggcatc atgacatgat cggctacacg gcggtcggcg ccggctcctc cgttgtgccg
     3541 gtacggttca actgtccgcc cgaggtcacg ctacatcgcc tgcggcgtcg ccgagagact
     3601 acagtcagta aggctcctcg cggaggttca gcctgaagag gatgcgcgac accgcgagtt
     3661 cgccgacagc gaagattgcc gtgccggcca caatgtcgag cacgcctatc ggcaccagcc
     3721 agctcgccgc gatgaacggc agaagcgatt ccggaatttg atcgagcccg atcgcttgac
     3781 tgctgggcgc aagtccgaac cgccgtttga tgaagctcga caagaggtcg cctgccatgg
     3841 cgcaagcggc caccaaggcg ccaagctccc agccgagccc catcagcgcc gacacggcag
     3901 gcgttgtcag aagtgcggca acgatgccgc ggattgtttt ggacacaccg aacagcgggt
     3961 ggccatctgt gagcgtcaaa cctccatcga gcggccagtc gagcgccgtc cccatcgcct
     4021 tcttcaagaa gagcggcgca gcgttggcaa cggcgatcag gacgaggagt tgaataatca
     4081 gtagcggctg catgacaccg ctattttatc cggcctgggc cggcagggga aaatgacctc
     4141 ttgcccttga ggggcggttt ggcccaatcc tgcgcaccag gatttcaacg gggtttctcc
     4201 tgatggcgag gcagacaggg cacacggcgc aggaaggcgc ggcgggtcag cggaccgcgg
     4261 tggcggtgct cgccgccatc agcgtgtgcc acatgctgaa cgacgtgatt cagtcgctga
     4321 tcctggcgat ctatcccatg ctcaagacga gccttgcgct cgacttcgcg cagatcggct
     4381 tcatcacctt caccttccag gcgaccgcgt cggtgcttca accgctcgtc ggttacgtaa
     4441 ccgaccgcta cccgacgccc tactcgctgg tggtcggcat ggcttcctcg ctggccggcc
     4501 tgatcctcat cgctttcgcc tcgtcctacg cgatggtact cggcgctgcc gcgctgatcg
     4561 gcacggggtc gtcggtgttt caccccgagt cgtcccgggt cgcgcggctc gcctcgggcg
     4621 ggcggcacgg cacggcacaa gcggtgtttc aggtgggcgg caatttcggg accgcgctcg
     4681 gaccgctgct cgcggccttc atcgtaattc cctacgggca aggctcgatc gcctggttct
     4741 cggtggtggc tctgctcggc atggcgctgc tgtcggtggt cgggaactgg tacggcaagg
     4801 cgctcaagtg gaaaccggtc tctcatcccg aagaacgcga ggcgattgcc gcgcttggcc
     4861 gccgcgaggt ggcgcgggca atcgccatcc tggtcacgct caccttctcc aagcacttct
     4921 atctggcctc gatcgcgagc ttctacatct tctatctggt ccaaactttc gacctgacgg
     4981 tgcagtcggc gcagctgttc ctgttcctgt tcctcggtgc cgtggcggca ggcaccgtaa
     5041 tcggcggtcc catcggtgat cgcatcggca cgcgccgcgt gatctggtgg tcgatcgtcg
     5101 gcgtgctgcc tttcacgctc gcgctgcctt atgtgggctt ggtgggcacg gccatactca
     5161 gcgtggtgat cggccttatc ctggcgtccg ctcacccggc catcattgtc tatgcgcaaa
     5221 gcctgttgcc gggccgcgtg ggcatggtgg cggggctgtt cttcggcctc gcgttcggca
     5281 ttgccggcat cggggcggcg ctgctcggcc ggctggccga cgccacgagc atcaccttcg
     5341 tctatcacgt gtgcgcattt ttgccggcga tcgggctgct tgcggcgttc ttgccagaga
     5401 tgtcaaaggc gaagcctcgg aagcggcaga agaaacgccc cgccacccgc tgaagtgcat
     5461 ccgatttgac aagatgatga cggctcatcc gcgtggcgga tcgaacttgt tccaaaagtg
     5521 tcttggtctt gtcttgggaa tttgctcctt gagcggccgg gcgggcgcag aataattacg
     5581 ccgatgctcg acataactgt ctgagtcgcc aaaggcgaga ctctgcagat ggttagggct
     5641 agaatgacgg cagtggcgcc gatgctgagg ctgtgtccgg cgcaattccg tacagtaatg
     5701 cgtaactgcc gagcggtgga cggcgggcct ttgtgtgctt gcgaaacatc cgattcggcg
     5761 tgtcgttctg tcgaggtgca gtaaccagag ggcattttga tgcgtgatat tcgcggggac
     5821 ctacaagacc gtgccagctt cctcaaggag cagctaagcg cggcgcaagc gcaattcgag
     5881 aaacgtcaag aacaactgaa gcgggagcac gaggcgagga tcgaggatct tcagtccgag
     5941 ttcgaggccg tcaccacgct catggagctg gaataccagc ggctgacgga cgcacccgtc
     6001 caagacgagc cgcggcaggc gccgccggtg gcggagcgcc atccggtgcg cgaggcagag
     6061 ccggatcgcc gccctgcccc ggaggccgag taccagcgcc gccaggagca ggagccttat
     6121 cgtcaggcga acggccgggc cactgagccg caacgccgcc cgtcacgtga gccggcgcag
     6181 taccgcgctc cgcatgcagc ggaggccgtg ccgcagaggc gccccggccc ggtaccggtt
     6241 cgccaagagc aagttcagga gcgtgttcat gagcgcgttc aagagcgggc tcatgaggac
     6301 gagggcgagc agcgttccgc tgcgccgcag atctatcggc gtgcgccttt ggccgccgcg
     6361 ccagcgcgcg agggcgaaca cgaacagccc cgtccggcgg aaagggtctc tcaagcaccc
     6421 cagcagccgc ctcttgcgga ctttctaatt cgcaagctta gcgagttggg cgctatgacg
     6481 tttgaggacc tctgccatat cgcggtgcag gaaggctatt tcgccaatgg cgatgtcgac
     6541 cgcggcgtgc acgtcacgct gacgaacgtc gcgaaggcag gcttcatccg gcagcagcct
     6601 gatgggacgt tctcgccggc gacggtcatg gacacgatcc gcctgcgtcg cgcgatctag
     6661 cagacaagcg cgcggctcgg actatcgcga gggccgcgcg cgcaacaact caacgacatc
     6721 gttgcggact ttcgtctccg tgtagcgcgc cagcgctttg cggtccggga agtcgtcgag
     6781 cggcacaggc gggccgatgc ggatatcggc gtcgagcggc ccaaggccga gcagcttcca
     6841 ggcatggctt gccatgtcca tgtctccgta ccaggcgacc tgtggacggc cgcgccgtcc
     6901 caagggcagg ccgtggagct tcgtgtaggt cagcgccagc gtctgcaccg aaaccttcga
     6961 tcccatgggc gcaccgcctg gcggcttgac cgctgcgaac aatgcggagc ggaacggcac
     7021 gacggagttg ccatcactgg acgtcccttc tgcgaagaac accacgtggt ctcccgcttc
     7081 caggcgctcc aggatttcgt tggccttggt gcccacttct gtgcgccgct cgcggtcgac
     7141 gaacaccgag cgttgcagct tggcgagcca tttcacgaac ggccaggtcg agacttcttg
     7201 tttggccacg aacgaaacgg gcgcgaccgc cgacagcacc gtgatgtcga gccacgagac
     7261 atgattggag acgatgagca cgccttgaga ctccgcgact tccccgtcga cattgaggcg
     7321 tatgccgatg atcctgcaaa cttgacggtg gtaccaatgc gggaagctgc gggcgaagcg
     7381 cgagccggta cgcaccagca ggtactgcag cggcatcaag gggagggtga aggcgaaaaa
     7441 tgtcgccagc accagaaatg cgcgcacgcg tttcatcgat atcgtttcaa gaacggccta
     7501 ccgcgcgtgt gctcaatagc agacccgctg accgtagcgt gtgacgcaaa gaccgcggag
     7561 ctgcggatcg gcgcgcatta tggggaccgg acgaacccgg cagtcggtct cgatttcgcc
     7621 ggcgccccag tcgcgggtcg tgcagacttc gcgcgtggcc gccgggacta tgtagaggtc
     7681 gagttcggac ggcatcttca cctcgacctt gggtgccggc accacaatcg ttgtggggct
     7741 tggagtcgag acaggctgcg cagggctggc cgtcacctca gtcacagacg ccgcagcgtt
     7801 gtccgtctcg accgaattgt cttccttggc cactggcgcg gcttccggct ccagagcatg
     7861 cccggcgaag gccatgccgc tcagcaatgt cacgatcgcg gcaagaacga tgcgtctacc
     7921 catcacgcca cccccttctt cattgattga cgcttcgcca aaattgtccc gccgcgcgcg
     7981 ggaagtcaaa cagggtgaaa taggcggtat attgcggcgc tatttgccgt tctttagggg
     8041 cgtgccgaag agttccaggc ggtgtccgat aagccggaag ccgagctcct tggcgacgcg
     8101 ttcctgcagc cgctcgatgt cctcattttt gaactcgatg acctcgccgg tcttcatatt
     8161 gatgaggtga tcgtggtggt cgtgcccggc cggctcgtag cgggcgcgcc cgtcgccgaa
     8221 gtcgtggcgc tcgacgatgc cctcgccttc catcaagcgc actgtccgat aaaccgtgga
     8281 tagcgagatg cgcggatcct ccgcgctggc gcggcgatag acctcttcca catccggatg
     8341 gtcgtgtgct tcggacagca cgcgggcgat gacgcgccgc tgaccggtca tgcgcaggcc
     8401 ccggtcggcg caaagccgct caagcctgct ttcccgtgtc gcctcgccgg acatggatcc
     8461 tttagggttg gtgggtttcg agcggctcat cgcaggtcgc gtccatcatc ggagggtggc
     8521 tggcaaaggg caagactcaa gatcgagact caagatcaac actcaggatc aagtctcaag
     8581 atcaggaccg aagatcaagg ctgaagatgg cggcgttcgc gccgctttcg taatagcgct
     8641 cccggcgacc ggcgggcttc gcgccagatg cgcggtaaag cgcaaccgcc gcggcgttgg
     8701 tctcgtcgac ttcgaggaag agttgcgtag cgccgcgcga cgacaacaca gcaagtgcgt
     8761 ggctcagaag cgcgcggcca agcccggcgc ggcggcgttc cggtatcacg cccatcgtca
     8821 gcagttccgc ttcgtcggcg gcagcccgcg cgatcagaag cgccgctggc gccgccacct
     8881 catggggtgc cgttccaaca agacacagca cgcccggcgc ggcgacgaac cgcgccatgg
     8941 cgatctcgtc ccaaggcttc gcgaagcagc gcgcatggag cgccgccaac agcgcggcat
     9001 cacccggacc ggcgcggctc acggcgcatg tgcgggtcat cagcgtcgct ccagcgtggg
     9061 cgcttgcggc ttggcgtccg gcggccgcaa ataaagcggc cgcaacacct cgcaggtctc
     9121 gcccctctca tgcgcaatct cggcaagcgc cgccgcgctc ggctgcagcg tctctaaagt
     9181 ggcgcccacg gaaagaccgc gggcggatgc agattcggcg agcgcgcctg cgccgcttcc
     9241 aaccgcgagc gccaaatttt tcgggagcat cgccgccgcg tcgtccggcg cgatgagcag
     9301 cggaccgtcc atcttagcgg cggtctcgtc gaagagaccg agatagagca tgccgcgcct
     9361 ggcatcgacg gcgacggcga acgggccttg agggtccgcg ccggccagaa gcgccgcgcg
     9421 cgccataacg ctcaaactgt cggtgccgaa aagcttcgcg ctcgtgacga gggcaagccc
     9481 gcgcgccgct gcaatggcga tacgcatgcc ggtaaagctg ccggggccga ctgtcgcggc
     9541 aatcaagtcg agttcgctcg cgccgatctc cgcctcggcc agcacctcgt ccaccatcgg
     9601 catgagcgct tcggcatgtc cgcgcggcat ctcgacctga agagcctcaa gcctccgcgc
     9661 atcgccagcg cgcaacaccg cggcggagca ggcgcccatg ctcgtatcga gcgctaagat
     9721 gttcatggct gcttcttaga cgatctgcgc catctggtcg aagtcgaagc gcggcgcgcg
     9781 cgggcgcaaa ggcgcatcgt tgccatagcc gagattgcac agaaaattgg atttcacatt
     9841 ggtcccggca aagaacgcca aatcgacgcc ctcttggtcg aagcccgaca tcggcccgca
     9901 atcgagccca agcccccgcg ccgcgaggat gagataggcg gcttgcagcg tgccgttgcg
     9961 aaaggcggag tcggcgatct tctgcggctt gccttcaaac caggccttgg cgtcggtgtg
    10021 cggaaagagc gtcggcagat gctcgtagaa gtcgaggtcg tagccgatga tggcgcagac
    10081 gggcgccttc atggtcttct cacgattgcc tgccgaaaga tacggctcca gcctctcctt
    10141 cgcgtttttg gatttcacga agacaaagcg cgcgggactg caattgcctg aggtcggccc
    10201 aagcttcacc aggtcgacca gctcatgcag cagagaatcg gggacgtcct tgtcctgcca
    10261 cgcgccgcgc gagcgcgcat cgcggaacag ttgatcgagc gatgcatctg gaagcttcgc
    10321 cgtcaagtcg gccggacctc ttgcacttca ggaataaagt gccggagcag gttctcgatg
    10381 ccgtgacgga gcgtcgccgt cgagctgggg cagcccgcgc aggcaccgcg catgtgcaaa
    10441 tagacgacgc cgtcgcgata gccctggaag gtgatgtcgc cgccgtcttg cgccacggcc
    10501 gggcgcacgc gcgtgtccaa gagttccttg atggtcttga ccgtctcttc gtcttcgggc
    10561 gcgtaggact cgttcacccc gtcatattgt gccgggtcgc cctcggtcat cgcctcgccg
    10621 gagaggtaat gctccatgat ggcgccgaga atggcgggct tcatatgctg ccactcgcca
    10681 tcgcccttgg tgacgctgat gaagtcggag cccaggaaca cgcccgtgac gccgtcaatg
    10741 tcgaacaggc gcgcggcgag cggagagcct tcgctttcgg tttggtcgcg gaactcgatc
    10801 gcgtctgcct cgccaagcac ggcccggccc ggaaggaact tcagggtcgc agggttcggc
    10861 gtcgcttcgg tctggatgaa catggcaatc ttctcggggt tcggggctgc tctacatcgt
    10921 tcaagattag aatagttcta caccaaaaat gtgtctgcag cgccgcaaaa tcaaggtgag
    10981 cgggcccctt ataccactat ctgaagtcga ttcgcttgcc cctagcctca ggccagcgcc
    11041 aggatttcct cggtcgtcag gtgccccgga accacggtga tcggcaccgg gaaggaccct
    11101 gcgagcttgc cgcccgccaa atgggtcacg agcggtccgg ggcctgaggg gtcccttgag
    11161 gcgccaagca ccaggacgcc gatatcctgg tcctcttcga tggcctttgt gatctcctcg
    11221 gccttactgc cttcccgcac gatctcctcc ggcacgagcg cctcgaagcc cagggccttg
    11281 agcttgcggc cgaacagccg gaacacggcc ttggctttgc tgaggccctc ctcgcgcgcc
    11341 atgtcctcaa cgcccagcca atgcagggcc tcgccttccg gctcgatgac gtagagcagc
    11401 gccagctggc ccttggtgcg cttggcccgg ctcgccgcga aggccaaggc gcgatcgcat
    11461 tccggggtct cgtcgaccgc caccaggaat ttgcgcttgt gaccctcaag atagatctcc
    11521 cgcttcttgc gcgcccgcgg tgtcgaagac gcggccttgg cagcaggcgc gggcttcttg
    11581 ggtgctgcat tcttgggtgc agccttttta ggcgcggctt tgcgcggcgc ggatttctgc
    11641 ttagccggcg gcgtcttctt cgccggtttc ttcttgggag tggccatggg cggcatgctg
    11701 ccacctccgg cgcccgccaa caaggcgcgt tgaagggggc gtggagcgcc tcagacgctc
    11761 aggcgcgttg ccttgcctag cggacgggat tgcggtttgg catcggtacg ttgacctggc
    11821 cgcccttgag cacgttgtga agctgcgtct tggtgaagag cggcacgccc ggcccggcct
    11881 ccttgctggc ggcggccttg agggccacgg gcggcaactc atcatcgacg ctgagcagct
    11941 cgtaatcgcc cgcggtgagc acggtccggc agtccttcgg cagatctctt aaggggtaag
    12001 gcttcttctt t
//
LOCUS       DBNHVU010000071         2406 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1885692, whole genome shotgun sequence.
ACCESSION   DBNHVU010000071 DBNHVU010000000
VERSION     DBNHVU010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1885692"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="AC16Y7_02735"
     CDS             <1..584
                     /locus_tag="AC16Y7_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708327.1"
                     /translation="VSRTYQDLGQFDLAREHAEKALQLARFGQTKGREALSLIRLADL
                     ARREGSTKAAAEYSAAAIECSNGPFTLPSAVASRGFALRGLSEGLQLMKQADRMLQTG
                     TFSSHNHFYVRVLQIEFALLREDWSAIEEAANKLDAHARAEPLPWTDFHIARGHVIAA
                     WGRNRMDEGCLRSLADLKKICNRTKMQIWLPGD"
     gene            1067..2260
                     /locus_tag="AC16Y7_02740"
     CDS             1067..2260
                     /locus_tag="AC16Y7_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HIR5708328.1"
                     /translation="MTIDRMALKALLEKGSDSDLLREMMAYVASRMMDAEVESLTGAG
                     LGERSAGRTNQRNGYRVREWHTGIGTVPVAIPKLRKGSYFPSFLEPRRAADKALVAVI
                     QEAYVHGVSTRSVDDLVKAMGMTGISKSQVSRLCEDIDGRVKEFLDRPIEGRWPYLWI
                     DATYVKNREGGRVVSVATIVAVGVNTDGRREVLGMANGPSEAEPFWSQFLRSLTARGL
                     RGVKLVISDAHEGIKAATKKVFHASWQRCRVHFMRNAMAHAGVKQRAMVAAAIRTAFV
                     QETEAAAREEWRAVADRLRAKFRKLAELMDSAEHEVLAYMAFPKSHWSQIHSTNPIER
                     LNREIKRRTNVVSIFPNEAAIVRLVGAILLEQNDEWAVSRRYMSLETLASFGDDAAVT
                     AITAD"
BASE COUNT          465 a          732 c          782 g          427 t
ORIGIN      
        1 atgtctcccg cacttatcag gatcttggtc aatttgatct ggcgcgcgag cacgcggaaa
       61 aagctctcca actggcccga ttcgggcaaa cgaagggtcg tgaagcgctc tccctgatcc
      121 ggcttgccga cttggctcga cgcgagggta gcaccaaggc cgcggctgag tattccgccg
      181 ctgcgatcga gtgctcgaac ggacccttca cgcttccgtc cgccgtcgca tcgagaggct
      241 ttgccttgag ggggttgtcg gagggactgc aactaatgaa gcaggccgat cggatgcttc
      301 agacaggcac tttctcaagc cacaaccact tctacgtcag ggttctgcag atagaattcg
      361 ctttgctgcg ggaagattgg agcgccatcg aggaagcggc caacaagctg gacgctcatg
      421 cgcgagccga acccttgcca tggactgatt ttcacatcgc acgtggtcac gtgattgccg
      481 cttggggacg caatagaatg gacgaaggct gtctacgctc cctggctgat ctcaagaaaa
      541 tctgcaaccg aacaaaaatg caaatttggt tgcccggaga ctgaaccgcg ggcacatcat
      601 cacatcgtga agtgtccgct aaatgggtca gaactcccga tctgctggtc gagtgccaac
      661 cgacgcggta gggccgaaag cggtcaccag acgccaaaat tcagcgctgt cgtgatgcgc
      721 tgcagcgatt ttcgaggcat cgacctgtga cttggtgtgc atggaaagca catctcgtct
      781 gatcgtccgt gcttgcgtgc ggccggttgt ggcccacagg actcgtcacc gccgatgaag
      841 gaagtctacg cgatcgggtc tacagcggag ggcgcgtccc agggcgtggt gtaagaggcg
      901 gggcggcggc gttgcgcgga gggagcccgt agggcgagcg cagggcaacg ccgcataaat
      961 cccttttccc caggacgtcc acagcggcga cgtcgagggg atcggtcatg ggccgactcg
     1021 ctgttggttc gggtttgcgc actcgaacag acaggatccg aatcccatga ccatcgatag
     1081 aatggcgctg aaggcgctgc ttgagaaggg ctctgacagc gatctcctca gggagatgat
     1141 ggcctatgtc gcctcgcgca tgatggacgc cgaggtcgag agcctgaccg gcgccggcct
     1201 cggcgagcgc tctgccggtc gcacgaacca gcgcaacgga taccgggtgc gcgaatggca
     1261 caccggcatc ggcacggtgc cggtggcgat ccccaagctc aggaagggct cgtatttccc
     1321 ctcgttcctg gaacctcgcc gcgccgccga caaggcgctg gtcgcggtca tccaggaggc
     1381 gtatgtgcac ggtgtctcga cgcgctcggt cgatgatctg gtcaaggcca tgggcatgac
     1441 cggcatctcg aaaagccagg tctcgcggtt gtgcgaggat atcgacggtc gcgtgaagga
     1501 gttcctcgat cgtcccatcg aaggccgctg gccctacctg tggatcgacg ccacctacgt
     1561 caagaaccgc gagggtggca gggtcgtctc ggtggcgacg atagtggctg tcggcgtcaa
     1621 cacggacggc cgccgcgagg tgctcggcat ggccaacggg ccgtcggagg ccgagccgtt
     1681 ctggtcccag ttcctgcgct cgctcacggc ccgaggactg cgcggcgtca agctcgtcat
     1741 ctcggatgcg catgagggca tcaaggccgc cacgaagaag gtgttccacg catcatggca
     1801 gcgctgtcgc gtgcacttca tgcgcaatgc gatggcgcac gccggcgtca agcaacgcgc
     1861 catggtcgct gccgccatcc gcactgcctt cgtgcaggag accgaggccg ccgcgcgtga
     1921 agaatggcgg gctgtcgccg accggctgcg ggcgaagttc cgcaagctcg ccgagctgat
     1981 ggatagcgcc gagcacgagg tgctcgccta catggccttc ccgaaaagcc attggagcca
     2041 gatccattcg accaatccga tcgagcggct caaccgcgag atcaagcggc gcaccaacgt
     2101 cgtctcgatc ttcccgaacg aagcggccat cgtgcgtctc gtcggcgcga tcctcctgga
     2161 gcagaatgac gagtgggccg tgagccgacg ctacatgagc ctggaaactc tggccagctt
     2221 cggcgatgat gctgccgtca cggccatcac ggccgactga ccccgagcct cagcgctcgg
     2281 accatgacgg tgctcttaca ccacagcttg gggcactacc gggtagagtg gacccgggac
     2341 cacgccgagt tcgtggaagc cgcagcgacg catgaggcga tgcgagcgcg tgttcggctc
     2401 gtcgac
//
LOCUS       DBNHVU010000072         6547 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_993512, whole genome shotgun sequence.
ACCESSION   DBNHVU010000072 DBNHVU010000000
VERSION     DBNHVU010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_993512"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2355
                     /locus_tag="AC16Y7_02745"
     CDS             <1..2355
                     /locus_tag="AC16Y7_02745"
                     /inference="COORDINATES: protein motif:HMM:NF018620.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid cis/trans isomerase"
                     /protein_id="HIR5708329.1"
                     /translation="HRLRFRARGAARAMLIASALLAASTTTGPAEEPAARSAAPDYTA
                     EIQPIFNARCIACHGCLGSPCNVKLSSFSGVERGGFGENPYSSHLESSPRTGMNVVQT
                     TAQWRKRGFYPVLSRDGSSQENVARSMLYQMVKVGAGHNKPGFSRKALMPVYAKRYDH
                     QCPATPEALGTHLKQHPAAGMPFGLPALDDKDVTKLRAWVAAGSPGPADKERRAAEMV
                     TEPAAVLAWENFFNASDKRSKLVARYIFDHVFMATIVLEESPGDFFKLVRSKTPSGSA
                     SANPPAPVEIIDTPLPYTDPYAHAGVDRFYYRLKKVTAPIIQKNHFVWRLKRSDIDHL
                     KDLFLGRDWGTTEHLDPPWGSGNPFLVFQAIPTEARYRFLLENSELIVSGITYGPVCL
                     GQTATFAVKDQFWVYFVDPKYDVSVLDPKLGLDTWDTFMDRSPLGNDAYEAAYAAALK
                     KLRPKGYTIDAIWNGDRKNSNAWLTVLRHESNVSVMKGRQGGIPRTQWLMNYGGFERI
                     YYDTVASFEYWSGDIPKLETLVFFNYLRQEFEDNFLLLLPEGARQKLRDDWTQGIGQA
                     ALALEPFAGADQPTQIKTNKNDPLLSLIADIQAHMGETVSGPPDRLNPRVKPDVSLRD
                     PIKSYDDWVAAASLLTQTQDHKFPRYLPSVILLKLNDGEDSRVYSLIANRVYASQDTI
                     LFQDGQSLPDLYTMSIYPTIVGGFPNYFFEMDLAQADDFLRGLRDVGSLADWNALRDR
                     YSVLRNDARFWPTYDWFTQWNFENRKSDAGYLDLSYYDLFDSVY"
     gene            complement(2366..3124)
                     /locus_tag="AC16Y7_02750"
     CDS             complement(2366..3124)
                     /locus_tag="AC16Y7_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018690304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR5708330.1"
                     /translation="MPLNAQQEQLCAENKTDFSDLTAVTVNCSLKRKLEDSHTRLLLS
                     VAEDIMRKSGVGVAQIHAAAHQIPSGVYPDMTEHGWDRDDWPSLWPQIEAADILIVAT
                     PIWLGEESSICRILIERLYGMSGLLNDKGQSLYYGKVGGTVITGNEDGVKHCAMSILY
                     ALQHLGYTIPPQADCGWIGEAGPGASYGDPSDAGPIGFDNDFTQRNTTIMTWNLMHLA
                     RILKDAGGYPNHGNNREAWKAGCRFGYENPDYRS"
     gene            complement(3733..4302)
                     /locus_tag="AC16Y7_02755"
     CDS             complement(3733..4302)
                     /locus_tag="AC16Y7_02755"
                     /inference="COORDINATES: protein motif:HMM:NF028235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5708331.1"
                     /translation="MARPREFDIDKALDRAMGVFWLKGYEGTSLQDLLKAMKIARGSL
                     YKAFEDKYSIYLAALDRYDQTEVEEGIAFLSDPKAGDGFSRVRAMLEGAKDDASHRGC
                     FLCNAAVDQARSDPEVGAKVSLMFRRLEEGIATALKQSRRAGRWSAKRRAATAAYILN
                     TYMGLRVLARSGYPPKDLQAIIDSALRTV"
     gene            4550..5893
                     /locus_tag="AC16Y7_02760"
     CDS             4550..5893
                     /locus_tag="AC16Y7_02760"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5708332.1"
                     /translation="MKYLKWLGLVLAILAVPVVAAYAIDRLFLAPDCPDCHTEATRAL
                     PLFDGTQDGLVRIQASTMEFRARAAGFDNAHGDGVILLHGFPETSVMWEPLLGELGDA
                     GYRAIAFDQRGYSPDARPSGQKEYTKGKIASDVLAVADAAGFDRFHVIGHDFGGIIGW
                     TVADRHPKRVLSLTSLSTPHPLAIAKALSNASSQWPRSSYVLFYWLPLLPELALGFDQ
                     AALLKSLKWRDHTNEQVEEYRRMFSEPGALHAALNWYRAFKFRSLDPVGKVRPPTLFI
                     WGREDPAFSRIAVLETANFMDGAYRLHPVPAGHNLLLEEPKIVTADILAHLDTATKVA
                     KQWAVALNEKPQDNDSPCDQAPPKCLRIVLSPNGGTFRIRNRCDDAYKGVVRISCSGW
                     APDAAVEYRFSLGAETEMAQESTGLSNGTCYYRHRLCAKKGAPPPQPRPAALFPF"
     gene            complement(5890..6282)
                     /locus_tag="AC16Y7_02765"
     CDS             complement(5890..6282)
                     /locus_tag="AC16Y7_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HIR5708333.1"
                     /translation="MIRSYSDHAANERTFLAWLRTGVAVIAFGFLIDRFDLFVRTMAA
                     AVSINPADRAQLEALSSHLGHGAGQGFVLVGIAFIIVATFRFVRTGYRLDDQKIHPPG
                     MATELSLSVILAVLLLVATIPLVFPGLF"
     gene            complement(6368..>6547)
                     /locus_tag="AC16Y7_02770"
     CDS             complement(6368..>6547)
                     /locus_tag="AC16Y7_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708334.1"
                     /translation="EELDRAKEAISASSDADLQLMALQATNNLRDDVLNDIFDGLGIS
                     KRPKIESVDWPALPL"
BASE COUNT         1257 a         2073 c         1977 g         1240 t
ORIGIN      
        1 catcggctac gcttcagggc acgcggtgcg gcgcgcgcta tgctcatcgc ctcagccttg
       61 ctcgcggcat ccaccacaac tggaccagcg gaggagccgg ccgcaaggtc ggcagcgccc
      121 gactacaccg ccgagatcca gcccatcttc aacgcacgct gcatcgcctg ccatggctgc
      181 cttggctcgc catgcaacgt caaactttcg tcgtttagcg gggtcgaacg cggtggcttc
      241 ggcgagaacc cctattcaag ccatctggag agctctccgc gcacgggcat gaacgtggtg
      301 cagacgacag cacagtggcg caaacgcgga ttctatcctg tcttgtcgcg cgacggctcg
      361 agccaagaga acgtcgcccg ctcgatgctc tatcaaatgg tcaaagtcgg cgcgggccac
      421 aataaaccgg gcttctcgcg caaagccctg atgcccgtct acgccaagcg ctacgaccac
      481 caatgcccgg cgacaccgga agccctgggc acgcacctca agcagcaccc cgctgccggc
      541 atgcccttcg gcctgcctgc gctcgacgac aaggacgtca cgaaacttcg ggcgtgggtg
      601 gcagcaggct caccgggccc ggcggataaa gagcgtcgcg ccgcggagat ggtgacggaa
      661 ccggccgctg tcctcgcctg ggagaatttc ttcaacgcat ccgacaaacg gtccaagctc
      721 gtcgcgcgct acatcttcga ccatgtgttt atggcgacga tcgtgctgga ggaaagtccc
      781 ggggatttct tcaagctcgt tcggtccaag acgccgtccg gcagcgcgag cgcgaacccg
      841 ccggctccgg tcgagatcat cgacacacct ctcccctaca ccgaccccta cgctcatgct
      901 ggggtcgatc ggttctacta ccgcctgaag aaggtcaccg cgccgatcat ccagaagaac
      961 cacttcgtct ggcggctcaa gcgttcggat atcgaccatc tcaaagacct gttcctgggc
     1021 cgcgattggg gcacgacgga gcatctcgat ccgccgtggg ggtccggaaa tcccttcctg
     1081 gtctttcaag ccatcccgac cgaagcgcgt taccgcttct tgctggagaa ctccgagctg
     1141 atcgtgtccg gcatcaccta cggcccggtc tgtcttggac aaaccgcgac cttcgcagtc
     1201 aaggaccagt tctgggtcta cttcgtcgat ccaaaatacg acgtgtcggt tctcgatccg
     1261 aaactgggac tggacacctg ggacacgttt atggaccggt cgccgctcgg aaacgacgcg
     1321 tacgaggccg cctacgccgc cgcgctgaag aagctccgtc ccaagggcta cacgatcgac
     1381 gccatttgga acggcgaccg caagaattcc aatgcgtggc tgacggtgct gcgccacgag
     1441 agcaatgtct cggtgatgaa gggccggcag ggcggcattc cgcgcacgca atggctcatg
     1501 aactatggcg gcttcgagcg catctactac gacaccgtcg ccagcttcga gtactggtcc
     1561 ggcgacatcc cgaagctgga aacgctcgtg ttcttcaact atctgcgcca ggagttcgag
     1621 gacaacttcc tgctgctgtt gccagaaggc gcgcgccaaa aacttcgcga cgactggacg
     1681 caaggcatcg gccaagccgc gctcgcgctg gagccgttcg ccggcgccga tcagccgacg
     1741 caaatcaaga ccaacaagaa cgatcctctg ctcagtctga ttgcggacat tcaggcccat
     1801 atgggcgaga ccgtcagcgg accgcctgac cgtttgaacc cgcgcgtcaa accggatgtg
     1861 tccttacgcg acccgatcaa gagctatgac gactgggtag cggcggcgtc actgctgacc
     1921 cagacgcagg accataagtt tccgcgctac ctgccgagtg tcatcttgct gaagctgaac
     1981 gatggcgagg actcgcgcgt ctattcgctc atcgccaatc gtgtctatgc ctcccaggac
     2041 acgatcctgt tccaggacgg ccagtccctg ccggacctct atacgatgag tatctatcca
     2101 acgatcgtcg gcggcttccc gaactatttc ttcgagatgg atctggcgca ggcagacgac
     2161 ttcctgcgtg gcttgcgcga tgtcgggtcg cttgccgact ggaacgcgtt acgcgaccgc
     2221 tacagcgtct tgcgcaacga cgcgcggttc tggccgactt acgactggtt cacgcaatgg
     2281 aatttcgaga accgcaagtc tgacgcgggc tatctcgacc tctcctacta cgacctcttc
     2341 gactcggtct attgagcgcg cgcgcctacg agcggtaatc ggggttctcg tagccgaacc
     2401 ggcacccggc cttccacgcc tcgcggttgt tgccgtgatt gggatagccg cctgcgtcct
     2461 tcaagatgcg cgcaaggtgc atcaggttcc aggtcatgat cgtggtgttg cgctgggtga
     2521 agtcgttgtc gaagccgatg ggcccggcgt ctgatggatc gccataggac gcccccggcc
     2581 cagcctcgcc gatccagccg caatcggcct gcggcgggat ggtgtagccg agatgctgaa
     2641 gcgcgtagag gatactcatg gcgcaatgct tcacgccgtc ctcgttgccg gtgatgaccg
     2701 tgccgccgac cttgccgtaa tagaggctct gtcccttgtc attgagcagg ccggacatac
     2761 cgtagagccg ctcgatgagg atgcggcaga tggaactctc ctcccccagc cagatcggcg
     2821 tggcgacgat gagaatgtcg gcggcctcga tttgcggcca caacgacggc cagtcgtcgc
     2881 ggtcccagcc gtgctcggtc atgtcgggat agacgcccga gggaatttga tgcgcggcgg
     2941 catggatctg cgccacgccc acgccgctct tgcgcatgat gtcctcggcg accgacagca
     3001 gcagccgcgt gtgcgagtct tccagtttgc gcttgagcga gcagttgacg gtgacggctg
     3061 tgagatcaga gaagtctgtc ttgttctcgg cgcagagctg ctcttgttgg gcattgagcg
     3121 gcatggcggg ggctcccagt cagtgacggc gacgggccca ctctatccgg cttctgagcg
     3181 caccggtgac cacattgaat aaactctcgg tgattgcccc cctcgatgca aaaaattgag
     3241 agtggccgca tgggagggga atgcggccac tctcctgagc cctgactatc gatccaggtg
     3301 ggtctgatag aggttcggga aaccttccgc ggcgacgggg gcgacgccaa cggtcatcag
     3361 ggcggtagca atcagaaggg tcttcagcat tttcgttctc cttagagagg ttggggcaag
     3421 gctctgcctt gggaggaaca ggggcctttg gtttttcgat ctagcgatcg aggttcgtct
     3481 ggtagacgtt cgggaagcct tccgcggcgg cgggggcaac gccggcggtg accaaggcca
     3541 ccgtaatcag gatcgtcttc aacattgtcg ttctccttcg agttggttgg ttgctctgaa
     3601 cgccccgttg atatcgggtt tggaaccgtt cgttcaacta acaaactctc acaaacgcgt
     3661 gtttgttgac cgattggtct ataaccaatc cgtgatgagg ccgtgaggag cttgagcggc
     3721 gcggggctcg cgctacaccg tgcgcaacgc gctatcgatg atggcttgaa ggtctttggg
     3781 cggatagccg gagcgcgcca ggacgcgcag gcccatatag gtgttcaaga tatacgcggc
     3841 cgtggctgcg cggcgcttgg ccgaccagcg ccccgcccgc cggctctgct tgagggccgt
     3901 ggcgatgccc tcctcaagcc gccggaacat caaggacacc ttggctccga cctccgggtc
     3961 gctgcgcgcc tgatcgacgg cggcgttaca caagaagcag ccgcggtgcg aagcatcgtc
     4021 cttcgcaccc tccagcatcg cacgcacgcg tgaaaacccg tcgccggcct ttgggtcgct
     4081 gagaaacgcg atgccctctt cgacttccgt ctggtcgtag cgatcgagcg cggcgaggta
     4141 aatgctgtac ttgtcctcga aggctttata gagactgccg cgggcgattt tcatggcctt
     4201 gagcaggtcc tggagcgacg tgccctcata gcccttcagc cagaacacgc ccatggcgcg
     4261 atcgagcgcc ttgtcgatgt cgaattcccg cggccgcgcc attcgccttc cccctcggcg
     4321 gatcatgggg gattcgtgac cgttcggtca actttttgcc ccggcgaaac accgagcccg
     4381 agcgaactca cagctttgct gcatcccacc taagccaaga aatcggccaa cattgccgct
     4441 aggttggcga tagggggcac gatctgatat ccatcagcac ccgttgtttg agtcgccggt
     4501 gaatcttggg gcccgggctc aggcttggag cagggtcggg ggacgcacca tgaaatacct
     4561 aaagtggctt gggctcgtcc tcgcgattct cgcggttccc gtcgtcgccg cctatgccat
     4621 cgatcggctg ttcctggcgc ctgattgtcc ggactgccac accgaggcga cgcgggcgct
     4681 tcccctgttc gacggcacgc aggacggctt ggtgcgcata caagccagca ccatggagtt
     4741 tcgcgcgcgg gcggcgggat tcgacaacgc gcatggcgac ggcgtgatct tgctgcatgg
     4801 cttccccgag acctcggtca tgtgggaacc gctgctgggc gaacttgggg acgcaggcta
     4861 ccgcgccatc gcgttcgacc agcgcggcta cagtccagac gcgaggccgt cggggcagaa
     4921 ggaatacacc aaaggcaaga ttgccagcga cgtgcttgcg gtcgccgacg cggccggctt
     4981 cgacaggttc cacgtcatcg gccatgactt tggtggcatc atcggttgga ccgtcgccga
     5041 ccgccatccg aagcgggtgc tcagcctcac ctccctgtcc acgccgcatc ctttggcgat
     5101 cgccaaagca ctttcgaacg caagctcgca atggccgcgc agcagctatg tcctgttcta
     5161 ttggcttccc ctgctgcctg agttggcctt aggcttcgat caagccgcac ttctcaagtc
     5221 cttgaagtgg cgggatcaca cgaacgaaca ggtcgaagaa taccggcgca tgttcagcga
     5281 acccggcgcg ctccatgccg ccttgaattg gtatcgggca ttcaaattcc gctccctcga
     5341 cccggttggc aaggttcgcc cgccgacctt gttcatctgg ggccgcgagg atcccgcctt
     5401 cagccgcatc gcggtcctcg agacagcaaa cttcatggat ggcgcctacc gccttcaccc
     5461 cgtccctgcg gggcataacc tcctactcga agaaccaaaa atcgtgaccg cggacatctt
     5521 ggcgcatctc gataccgcga cgaaagtcgc caagcaatgg gccgtggcgc tgaatgagaa
     5581 gccccaagac aacgactcgc catgcgacca ggcgccgccg aaatgcctgc gcatcgtcct
     5641 gtcaccgaac ggcggcacgt tcaggatccg caatcgctgc gacgatgcgt acaaaggcgt
     5701 ggttcgcatc tcctgctcag gctgggcgcc cgacgccgca gttgagtatc gcttcagcct
     5761 cggcgccgag acggagatgg cacaagagag cacgggccta tccaacggca cgtgctacta
     5821 ccggcatcgg ctttgcgcga agaagggtgc accgccgccc cagccccgcc ctgccgcgct
     5881 gttcccgttc tagaacagtc ccgggaagac caatggtatc gtcgcgacaa gcagaaggac
     5941 ggcgaggatc accgacaggc tgagctcggt ggccatgccc ggcgggtgaa tcttttgatc
     6001 gtcgaggcgg taacccgtcc gcacaaagcg gaacgtcgcc acgatgatga aggcaatgcc
     6061 gacaagaaca aagccctggc ccgcgccatg accgagatgg ctcgacaagg cctcgagctg
     6121 cgcgcgatct gcagggttga tcgaaactgc ggccgccatg gtgcgcacga agagatcgaa
     6181 ccggtcgatg agaaagccga acgcaatgac ggcaacaccg gtgcgcagcc acgccaggaa
     6241 ggtccgctca ttggcggcgt ggtcggaata ggatctgatc atgccgagcc tcggtcctct
     6301 aaagctgcgc tagcttggcc gaaaagcgcc aagaatggct taaagccgcc agcagagccc
     6361 tggaaggcta aagtggcaag gccggccaat ccacgctttc aatcttgggc cgcttagaaa
     6421 tcccgagacc atcgaagatg tcgttgagca cgtcgtcgcg caaattgtta gtggcctgca
     6481 atgccatgag ctgcaagtca gcgtcgctgc ttgcacttat ggcctccttg gcgcgatcaa
     6541 gctcctc
//
LOCUS       DBNHVU010000073         8149 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1217857, whole genome shotgun sequence.
ACCESSION   DBNHVU010000073 DBNHVU010000000
VERSION     DBNHVU010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8149)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8149)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8149
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1217857"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /gene="purH"
                     /locus_tag="AC16Y7_02775"
     CDS             <1..285
                     /gene="purH"
                     /locus_tag="AC16Y7_02775"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170831.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR5708335.1"
                     /translation="IGAGQMSRVDAARIAARKATDAADALGLKEPLTTGSVAASDAFF
                     PFADGLQVVAEAGATAVIQPGGSMRDDEVIAAADEAGLAMVFTGMRHFRH"
     gene            complement(374..847)
                     /locus_tag="AC16Y7_02780"
     CDS             complement(374..847)
                     /locus_tag="AC16Y7_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708336.1"
                     /translation="MKYIGMIAAVVAMGLFAACEQPKDDDADKPAEAPAAEEGMTEES
                     TSTSPMVSEDAPSEPHMGEPPSPNQPQPVYPDGAAMDADGADRVMVEEVMVEETIVVA
                     PTVSPDAPSEPHMGEPPSPNQPQPVVPGDGGGAAASGEPASSSQPQPAYPDGAPQ"
     gene            complement(935..2257)
                     /locus_tag="AC16Y7_02785"
     CDS             complement(935..2257)
                     /locus_tag="AC16Y7_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5708337.1"
                     /translation="MFDWATQPFYTLIVTFLFAPYFVNGFMDDPAYGQTLWAYATGAA
                     QLVAAALAPVLGAIADAGLPRKPWIAGFSVLLVVGLSGLWFAAPGDLSAVPLVLLCFG
                     IATIGAELATVFNNAMMPDLVPDKRLGMLSSVGWATGYVGGLVSLALVAGLIVTDPNT
                     GKTLFGLEPIFALDAAAHEGDRLVGPFSAAWYLIFALPLFLFTPDRRGGAPGPGVVRE
                     GLRQLRDSVRNLMRNHRNVALFLLARMLYADGLGAIFAFGAIYAASIFGWGTAELGLF
                     GIVLTVTATIGACVGALLDDRTGSKRVIVFTLVLFIVGSIGVLSVDPDEVLFFVPVTP
                     KANGSGLFASAGERAYLAFAVLIGLASGPVQASSRALLARLCPPDKTTEFFGFFAFSG
                     KITAFAAPLAIGAATALTGSQRLGISASIVFLIGGLLLLFWVRAPRGS"
     gene            complement(2301..2933)
                     /locus_tag="AC16Y7_02790"
     CDS             complement(2301..2933)
                     /locus_tag="AC16Y7_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR5708338.1"
                     /translation="MKPFSSDEHAVIADAISRAERTTSGEIVVVVARASSGYFFFALM
                     CVALAALAVPLPLIHFTKWPIEYVYLLQLAVFGLGVLIARWERFRVAIAPRGLKQARA
                     HLRAVEQFLAQNLHTTKGRTGVLIYVSDAERYAEVIADDGIYKKVSPEIWDELIEDLT
                     AQIGRGNRVEGFVAAIETCGAILAEHFPPEPLGEDELPNHLIVIDGAKLP"
     gene            3049..3759
                     /locus_tag="AC16Y7_02795"
     CDS             3049..3759
                     /locus_tag="AC16Y7_02795"
                     /inference="COORDINATES: protein motif:HMM:NF022694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HIR5708339.1"
                     /translation="MQTRHLSFLLCAGLTLGGFAVSKMALARGPDMAGGVAFPFGPNL
                     SASGWQSVRSPWRRGARFSAHGKDALVVETAGGFGLLWHAVPRQVAKAEQARWRWRKQ
                     EGVGPTDLTKKGGDDRVLALYFAFANPADVTPGTDLSELLRSGHGQVLIYVWGGAAKP
                     GTVLPLPYFDGRGRTIVKRAANAKPGVWFDEEAGLRTDFARAFGRPPGHLVAVGVSSD
                     ADDTGGRNVVALADLCVK"
     gene            complement(3768..4523)
                     /locus_tag="AC16Y7_02800"
     CDS             complement(3768..4523)
                     /locus_tag="AC16Y7_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009211406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR5708340.1"
                     /translation="MATTALRVVLFLVALCVAAAPATSAPEFPALSGRVVDQADLLSP
                     EDERELTAHLKALEEKSSDQLVVVTVPSLQGYTIEDFGYQLGRHWGIGTAELDNGALL
                     IVAPNERKVRVEVGYGLEPILTDALSNIIIENAVLPRFRAGDFAGGIKDGVRDLTLSL
                     TGDAAEVEARARARHDADKVPIDWFTILLYFAIFLLLMYLMRNSTYVVPEGSSRRNDR
                     SGWGSGGGWSGGGGGGFSGGGGGFGGGGSSGSW"
     gene            complement(4542..5099)
                     /locus_tag="AC16Y7_02805"
     CDS             complement(4542..5099)
                     /locus_tag="AC16Y7_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR5708341.1"
                     /translation="MLASVLLAGCGINQIPTLDERAKAAWSQVLNQYQRRADLIPNLV
                     ETVKGFAEQESKVLTEVIEARAKATQVQIPKDILTNKEAFDKFQDAQNNLSGALGRLL
                     VVVERYPDIKSNQNFLALQSQLEGTENRIAVARRDYITSVRDYNTELRTIPGRWWRAL
                     LYPESEPMQNFSVSEETTKVPQVEF"
     gene            5600..7597
                     /locus_tag="AC16Y7_02810"
     CDS             5600..7597
                     /locus_tag="AC16Y7_02810"
                     /inference="COORDINATES: protein motif:HMM:NF004785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR5708342.1"
                     /translation="MTTAARTLTTFSIFAGAVALATMVAVAPADAKKTKTPKDEQAAP
                     AADKNQPMTIIISLRNQKANIYRGMSLVTSTRVSTGKRGYSTKPGVYSILEKRRRHYS
                     NLYAGAPMPWMQRLTWTGTALHAGVVPGYPASHGCVRLPYSFAPKLFKMTGVGEQVVV
                     ARNMVAPKLIDHPALFQPLPPPLPPMMAEEHEQHQMRRSSNDAVPALGTRLPVVFAKA
                     EAVVPSAKAPATATIIARALLAQRETPAQRPMVLAVLEDTRTHAIDPTAEPFMGSGSH
                     AFAAKPSSDETQASVGTSDDFFKHRDRLATPAEAPVAPAISANAVEPEAPAPAEASMA
                     MEAAVAADPREPEVAREPEVASEPEAGPILTVSASALSQPAAKFATVEIAALGPEDHP
                     PMPPAKPSVIAAKLAAGASAAAVEAAEPRSTEPLRVLITRRTKRDRMIGVQYILASMG
                     HLDHQEFDGTFGQKTIRAIKAFQKANDLPETGAFTDELEKKVYEVAGKKQPPSGHLFV
                     RQKFVGVFDTPVRFRNPDAPLGTHVFTVMHFGKDATEAQWTAISLKDDADPTIVLDRI
                     EIPDDVRQNISERLTPGSSLIVADTAINSATLPKGADFLVWDTSKPAKVQRASVSPRP
                     RKKRRRTTSRRRTQPSYTRRAAPTYSRRSTQRRYRRGLYPF"
     gene            complement(7654..7728)
                     /locus_tag="AC16Y7_02815"
     tRNA            complement(7654..7728)
                     /locus_tag="AC16Y7_02815"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7694..7696),aa:Gly,seq:gcc)
     gene            7893..>8149
                     /locus_tag="AC16Y7_02820"
     CDS             7893..>8149
                     /locus_tag="AC16Y7_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HIR5708343.1"
                     /translation="MPSDRATLFARLEELGIASKTVEHAPMFTVEQSQNLRGTIPGGH
                     TKNLFLKDKDGRMALVIAKDATKVDLKALARQLGFGRFSFG"
BASE COUNT         1551 a         2643 c         2586 g         1369 t
ORIGIN      
        1 attggcgcag gccaaatgag ccgcgtggat gcggcgcgca ttgccgcgcg caaagcaaca
       61 gatgcggccg atgcgctagg gctgaaggag ccgctgacga ccggctcggt cgcggcttcg
      121 gatgccttct tcccgtttgc cgatgggctt caggtggtgg cggaagcggg cgctactgcc
      181 gtcatccagc cggggggctc catgcgtgac gacgaagtca tcgccgcggc ggatgaagcc
      241 gggcttgcca tggtgttcac aggcatgcgg cacttccggc actagcacct caagtcgtgc
      301 gggcggaaat acgcacgcaa aaaggcctcc cgaaacggga ggcctttgct ttgctgttag
      361 cggccgttgg cgcctattga ggcgcgccat ccggataggc cggctgcggc tggctggaag
      421 aggccggctc accggacgct gcggccccac cgccgtcacc cggaacgacc ggctgcggct
      481 ggttcggcga aggcggctcg cccatatgcg gctcggacgg tgcgtccggg gaaacggtgg
      541 gcgcgacgac gatggtctcc tccaccatca cctcttccac catcacgcgg tcagctccgt
      601 cggcatccat ggccgcgccg tccggataga ccggttgcgg ctggttcggc gagggcggct
      661 cgcccatgtg tggctccgac ggcgcgtcct cggagaccat aggcgaggtg gaggtgctct
      721 cctccgtcat gccctcttct gctgccggcg cctcagccgg cttatccgcg tcatcgtcct
      781 tcggctgctc gcaggccgcg aaaagcccca tcgcgaccac tgccgctatc attccaatgt
      841 atttcataat ccccctccca cgatctcggt ggaattcaac accccatctg gcgaaaacat
      901 agcgcgaacg cagcggcttt gctacggcgc atgtctagct tccgcgtggg gcgcgaaccc
      961 agaatagtag tagcagcccg ccgatcagaa aaacgatgct ggcggagata cctagccgct
     1021 ggctgcccgt gagggccgtc gccgcgccta tggcaagcgg cgcagcgaac gcggtaattt
     1081 tccctgaaaa ggcaaagaag ccgaagaact cggttgtctt gtcaggcggg caaaggcggg
     1141 ccagcaacgc gcggctcgag gcctggaccg ggccggaggc aagcccaatg agaacggcga
     1201 aggcgagata ggcccgctcg cccgcggagg cgaacagtcc gctgccgttt gccttcggcg
     1261 tgaccggcac gaaaaacagg acctcgtccg gatcgaccga aagcaccccg atcgagccca
     1321 cgatgaagag gacgagggtg aaaacaatca cccgcttgga gcctgtccgg tcgtcgagaa
     1381 gggcaccgac gcacgccccg atcgtggcgg tgacggtgag cacgatgccg aacaggccga
     1441 gttccgccgt gccccagcca aatatgctcg ccgcgtagat agcgccgaag gcgaatatcg
     1501 cgcccagacc gtccgcgtag agcatgcgcg ccaacaggaa cagcgcgacg ttccggtgat
     1561 tgcgcattag attgcgcacc gagtcgcgga gctgccgcag accctcccgg acgacaccag
     1621 gccccggcgc cccgccccgc cggtccggcg tgaacagaaa gagcggcagg gcgaagatca
     1681 gataccaagc cgccgagaac ggtccgacaa gccggtcgcc ctcgtgcgcc gcggcgtcga
     1741 gggcgaagat cggctccagg ccaaacagcg tcttgccggt gtttgggtcc gtgacgatga
     1801 ggccggcgac gagagcgagg ctgacaagcc cgcccacata gcccgtcgcc cagccgacgc
     1861 tcgacagcat gccgagccgc ttatccggga ccagatccgg catcatggcg ttgttgaaca
     1921 cggttgcgag ctcggcgccg atggtcgcga tgccaaagca cagaaggacc agcggcacgg
     1981 cggagagatc gccaggggcg gcgaaccaca gcccgctcag tccgacgacg agcagaaccg
     2041 agaagccggc gatccatggt ttgcgcggca agccggcatc ggcaatagcg ccaagcacgg
     2101 gcgcaagcgc ggcagcgacg agctgcgcgg cgcccgtggc gtacgcccac agcgtttgac
     2161 catatgcggg atcgtccatg aacccattca cgaagtacgg cgcgaacagg aaggtgacga
     2221 tgagcgtgta gaagggctgc gtcgcccagt cgaacatcgc ccagccgaca aggccgcgcc
     2281 tgctcgttgt atatgccgta ctatgggagt ttggcgccat cgatcacgat cagatgattg
     2341 ggaagctcgt cctcgccaag aggctcgggg ggaaagtgtt cggcgaggat cgcgccgcat
     2401 gtttcgattg ccgcaacaaa accctcaaca cggtttcccc ggccgatctg cgcggtcaaa
     2461 tcctcgatga gttcgtccca tatttctggc gacaccttct tgtatatgcc gtcgtcggcg
     2521 atcacttcgg cgtagcgctc cgcatcggac acatagatga gcacgccggt gcgccccttc
     2581 gtggtgtgca gattctgcgc gagaaactgt tcgaccgcgc gcaaatgtgc acgcgcctgt
     2641 ttcagcccgc gcggtgcgat ggcgacgcga aagcgctccc atcgcgcgat gagcacgccg
     2701 aggccgaaga cggcaagctg caggagatac acatattcga taggccactt cgtgaagtga
     2761 atgagcggaa gcggcacggc gagcgcggca agcgccacgc acatcaacgc aaagaagaaa
     2821 taaccggagg aggcacgggc aactacgacg acgatctcac cgctcgtcgt cctctcggcg
     2881 cgggaaatag catcggcgat cacggcatgt tcatcgctgc tgaacggctt catcgcgtat
     2941 cgtcttcccg gggtcgctca ttccatgcgc cggcgcacac tatggcgata atcgcgcaca
     3001 ttggaaccgg caatcccatg cgaaccgttg cggcttttcg aggaacacat gcagacgcgg
     3061 cacctttctt ttctcctatg cgcaggactg acacttggcg ggtttgccgt gtcgaagatg
     3121 gcgcttgcga ggggaccgga catggccggc ggtgtcgcat tcccctttgg gcccaatctt
     3181 tccgcctccg ggtggcagtc ggtgagatct ccgtggcggc gcggcgcgcg attttccgcg
     3241 cacggcaagg acgccctggt cgtggagacg gcgggcgggt tcgggcttct gtggcatgcc
     3301 gtgccgcgcc aagtggcaaa ggcggagcag gcgcggtggc gctggcgcaa gcaggagggc
     3361 gtgggaccaa ccgatctcac caagaagggc ggcgacgacc gcgtgctcgc gctgtacttc
     3421 gcctttgcca atccggctga tgtgacaccg ggaaccgacc tctcggagct cttgcggtcc
     3481 ggacatggcc aagtcctcat ctatgtctgg ggtggggcgg ccaagcccgg gaccgtcttg
     3541 ccgctgccct atttcgacgg ccgcggccgc accatcgtga agcgtgcagc gaatgccaag
     3601 ccgggggtct ggttcgatga agaggccggg ttgcgcacgg actttgcgcg ggccttcggc
     3661 aggccgcccg gtcatctggt ggccgtcggc gtttcgagcg acgccgacga tacgggtggc
     3721 cgcaacgtgg tcgcgctcgc ggacctctgc gtgaaataga cggagcctta ccagctcccg
     3781 gacgacccgc cgccgccaaa gcctccgccg ccaccggaga aaccaccgcc tccgccgccg
     3841 ctccagccgc cacctgagcc ccaaccggaa cggtcgttgc ggcggctact gccttccggc
     3901 acgacatagg tgctgttgcg catcagatac atgaggagca ggaagatcgc gaagtagagg
     3961 aggatcgtga accaatcgat gggaaccttg tcggcatcgt gcctggcacg cgcgcgcgct
     4021 tcgacttccg ccgcgtctcc ggtcagcgac aatgtgagat cgcgcacgcc atctttgata
     4081 ccgccggcaa aatcgccggc gcgaaagcgc ggcaggaccg cgttctcgat gatgatgttg
     4141 gaaagtgcgt cggtgagaat gggctcaagg ccatagccaa cctcgacgcg gaccttgcgt
     4201 tcgtttggcg caacgatcag gagtgcgccg ttgtcaagtt ccgcggtgcc gataccccag
     4261 tgccggccga gctgatagcc gaagtcctcg atggtgtaac cctgcagcga tggaaccgta
     4321 acgacgacga gctggtccga gctcttttcc tccagcgctt tcagatgcgc ggtgagctct
     4381 cgttcgtcct caggacttag aagatccgct tgatccacca cgcgtccgga gagcgcggga
     4441 aactccggcg cggatgtcgc gggcgcagcc gcgacgcaga gcgcaaccag aaataggaca
     4501 acacgaagag ccgtggtggc catgagggcc ccgcttcgcg gctagaactc cacctgcgga
     4561 accttcgtcg tctcttcgga gacggagaag ttctgcatcg gttcggactc cggatagagc
     4621 agtgcgcgcc accagcggcc ggggatcgtg cgcagctcgg tgttgtagtc ccgaacggag
     4681 gtgatgtagt cgcgccgggc aacggcgata cgattctcgg taccctcaag ctgcgactga
     4741 agcgccagga aattctggtt cgacttgatg tccgggtagc gctcgacaac gacgagcaga
     4801 cggcccaacg cgccggaaag gttgttctgc gcgtcctgga atttgtcgaa ggcttccttg
     4861 ttggtgagga tgtccttcgg gatctgcacc tgcgtggcct tggcgcgcgc ctcgatcact
     4921 tcggtgagca ctttgctttc ctgctcggcg aagcccttca cggtctccac aagattggga
     4981 atgagatcgg cacggcgctg atactgattg agcacctggc tccatgccgc cttggcgcgc
     5041 tcatcgaggg tcggaatctg gttgatgccg cagccggcca gcaggactga cgcaagcatg
     5101 agaacccctg cgcggccgac aggcccagac caaagacgcg ccaatacgtg catgacatcc
     5161 tcccgcgtgc agcgaaacgc agacctcgtg tattggggcc ctgcgcaact ggcaagtgct
     5221 ttccacaacc ggcgcaacgc cttgccagcg gctggcgggg ccggtagggg cctcggaagg
     5281 tgttgcgaga atgaaacaac gcccaagttt cttcgccgac cgggggcttt gggggatcgg
     5341 cccactttgg tccacgcatt attgggagcc cttaagtccc ggcagttccg cgattttttc
     5401 gatacccgcg atcgtccacc ggaatcccac aagtcccgga ggacgttagt taatccgaag
     5461 gctttgctag tcaaagtcgg gggcctcttc tagtgtgatt cgcgagtcgt tttgggggac
     5521 tcgcggaggc tcgctatgag accgatttct tacgggggag cgagtacggt gtgtatgcgt
     5581 aaagcagggg aatgaggcgg tgacaaccgc agcgcgaact ctcacgactt tctcgatttt
     5641 tgccggcgcc gtggccctcg cgacgatggt cgccgttgcg cccgccgacg cgaagaaaac
     5701 caagacgccc aaagacgagc aagcggcacc ggcggccgac aagaaccagc cgatgacgat
     5761 catcatttcc ttgcgaaatc agaaggcgaa tatctaccgc ggcatgtcgc tggtcacgag
     5821 cacgcgggtc tcgaccggca agcgcggcta ttccacgaag cctggcgtct acagcattct
     5881 ggagaaacgt cggcgccatt actcgaacct ctacgcgggc gcgccgatgc cctggatgca
     5941 gcggctgacc tggacgggca ccgcgctcca cgcaggcgtc gttcccggct atcccgcctc
     6001 acatggctgc gtccggctgc cctactcgtt cgcgcccaag ctgttcaaga tgaccggcgt
     6061 cggcgagcag gtcgtcgtcg cccgcaacat ggtcgcgccc aagctgatcg atcaccctgc
     6121 cctgttccaa ccgctgccgc cgcccttgcc gccgatgatg gctgaggaac acgagcagca
     6181 tcaaatgcgc cggtcgagca acgacgccgt acccgctctg ggcacccgcc tgccggtcgt
     6241 cttcgccaag gccgaagcgg tcgtgccgag cgccaaggca cccgccacgg cgacgatcat
     6301 cgctcgagcc cttcttgctc agcgggaaac ccctgcgcaa cgcccgatgg ttctggcggt
     6361 ccttgaggac acgcgcactc acgccatcga cccgacggcg gagccgttca tggggagcgg
     6421 ctcgcacgcg ttcgctgcaa agccgtcgtc agacgagacg caagcgtcgg ttggaacctc
     6481 ggacgatttc ttcaagcatc gggaccgcct cgcgacgccc gccgaagcgc cggtcgcgcc
     6541 tgcgatttcc gcaaacgccg tcgagcctga agcgccggcc ccggccgaag cgtcgatggc
     6601 aatggaggcc gccgtggcgg cggatccgcg tgagcccgaa gttgcgcgcg agccagaggt
     6661 ggcgagcgag ccagaggccg gaccgatctt gaccgtgtcc gcttcggcgc tgtcgcagcc
     6721 tgcggcgaag ttcgcgaccg tcgagattgc cgcgctggga ccggaagacc atcctcccat
     6781 gccgccggcc aagccatcgg tgattgcagc caagctggcg gcgggagctt ccgctgcggc
     6841 cgtcgaggca gccgagccgc gctccaccga gccgctccgc gtcctgatca cgcgccgcac
     6901 caagcgggat cgcatgatcg gcgtgcagta cattctggcg tccatgggtc atctggatca
     6961 tcaagagttc gacggcactt tcggccagaa gacgatccgc gccatcaagg cgttccagaa
     7021 agccaacgat ctgcccgaga ccggcgcctt caccgacgaa cttgagaaga aggtctatga
     7081 agtcgccggc aagaagcaac cgccgtccgg tcaccttttc gtccgtcaga agtttgtggg
     7141 cgtgttcgac acccccgtcc gcttccgcaa ccccgacgcg ccgctcggca cgcacgtctt
     7201 cacggttatg catttcggga aggacgcgac cgaggcgcag tggacagcga tcagcctcaa
     7261 agacgatgcc gatcccacga ttgtgctcga tcgcatcgaa attcccgacg atgtccgcca
     7321 gaacatctcc gagcggctaa cgcccggctc ctcgctgatc gtcgccgata ccgcgatcaa
     7381 ctcggcgacc ctgccgaaag gcgccgactt ccttgtttgg gatacgagca agcccgccaa
     7441 ggtgcagcga gcgagcgtgt cgccgcggcc tcggaagaag cggcgccgca cgacaagccg
     7501 ccgccgcacg cagccgtcct atacgcgccg cgccgcgccg acctattcgc gccgctccac
     7561 gcagcgccgt tatcgccgcg gtctctaccc cttctagggc agcgaccgac ggcgaccggc
     7621 gggtcgaact aactttcgca ttttttagaa aattggagcg ggcgatggga ttcgaaccca
     7681 cgaccccaac cttggcaagg ttgtgctcta cccctgagct acacccgcac tccaaaccac
     7741 cggcaagcct tgaatttcac gcttcagggt ggtcatttgc cttgtagtgg atcgcgcctg
     7801 aactgtctac ttcagccgcc ttccccaaaa ctatgacccc ttcaggggcc catcaagggc
     7861 acccccatta gccgaagccc acgccgaaag caatgccatc cgatcgcgcc acgctgtttg
     7921 cccggctgga ggagctgggc attgcctcca agacggtcga gcatgcgccg atgttcaccg
     7981 tcgaacagtc ccaaaatttg cgcggcacga ttccgggcgg ccacaccaaa aatctgttcc
     8041 tcaaggataa ggacggccgc atggcgctcg tgatcgccaa ggacgccacc aaggtcgacc
     8101 tcaaggcgct ggccaggcag ctcggctttg gccgtttcag tttcggcaa
//
LOCUS       DBNHVU010000074         5924 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_252469, whole genome shotgun sequence.
ACCESSION   DBNHVU010000074 DBNHVU010000000
VERSION     DBNHVU010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5924)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_252469"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="AC16Y7_02825"
     CDS             <1..460
                     /locus_tag="AC16Y7_02825"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR5708344.1"
                     /translation="FSTYIVSGGGIEFVRNFSEPVYGIPPAQVVGSSIKTEYKVVDGK
                     PKIMRLPEVNFIDDKAGKPVGINQHIGKRPIFASGNSDGDFEMLEWTTAGDGPRFGLI
                     VHHTDGEREYAYDRESHVGRLDKALDAAADNGWVVIDMKNDWKEIFPPAD"
     gene            670..960
                     /locus_tag="AC16Y7_02830"
     CDS             670..960
                     /locus_tag="AC16Y7_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR5708345.1"
                     /translation="MISKTELIQRIADELEMTKRDVKNVLETLATVGYKEMKKTGEFM
                     LPGFAKFVVVKKPATKARKGINPFTKEPTVFKAKPARKIVKARPVKAAKDAV"
     gene            complement(957..1262)
                     /locus_tag="AC16Y7_02835"
     CDS             complement(957..1262)
                     /locus_tag="AC16Y7_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708346.1"
                     /translation="MPATIQSRHEDERAGAAASGFAAGAGCLACFLGCFLVLEGDLRR
                     RVTVLPYSDFARGRKGKREDARKSGPASREAKQGVVPASKGGDTFPLAHTFERREPC"
     gene            1252..3582
                     /locus_tag="AC16Y7_02840"
     CDS             1252..3582
                     /locus_tag="AC16Y7_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5708347.1"
                     /translation="MAGIAAAAVLAAGLLWQSLDRKPTGSADTSPETLAFVGSETCIG
                     CHQEAGKLWHGSQHAHAMAHATAKTVLGDFDDATFVYGDVTSRFFKKDGKFFVETDGP
                     DGDLETFEVKYTFGLEPLQQYLIEFPDGRVQSLTIAWDSRPKEEGGQRWYHLYHDEII
                     AHDDPLHWTKLQQNWNFMCAECHSTGVAKNYDPATDSFDTTWKEISVGCEACHGEGYA
                     HVAWANTWSPFAKKDKAKGLFAAFDERTNVTWTQNKKTGQPVRDPRPTHLRKEVEMCG
                     RCHARRGQISEAFVPGRPLSDTHTVGLITRDLYQPDGQKRGEVYNYGSFKQSKMFAAG
                     VTCSDCHEPHSASLRAKGDQVCLQCHAPHYAETSHTHHEGENAPGCVACHMPAREFMV
                     IDTRHDHSFRVPRPDLSVTLGVDNACTDCHTGKSSEWAAAAIEDWFGPERKGFQTFAP
                     AFQAAWEGAADTEALLQAVATDPKTPAFARASAFQELTAFPSVETARAVREGLADPDP
                     LVRLAALDHLETAQPGRLWPIVSPLLDDPVRGVRIKAALLLAGTPQQALQPADRARLA
                     KAEKEFMAAQRLNADRPEGRAVLGRYYARGGNVAKSEEEYRAALRLSPHFAPAAVNLA
                     DLYRQSGRDEDGVAVLREALKLSPDAGGLHHALGLALVRLKKSDEALESLRLATELDP
                     DRARYAYVYAVALDSQGRRPEAIEVLKESLKRHQSDRQTLGGLINFTRADGDTAAALR
                     YTEQLLKLEPENPNLKQLIKALKDADDQGSDDQGAQ"
     gene            complement(3593..4318)
                     /locus_tag="AC16Y7_02845"
     CDS             complement(3593..4318)
                     /locus_tag="AC16Y7_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035444 - nickel cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt transporter"
                     /protein_id="HIR5708348.1"
                     /translation="MQEIIALQRWLYEGMADGLGEVAQGGALALPAVMAGAILFGFLH
                     AMMPGHGKTVLFSYHLGQKGSLLSSIANGAILALTHVGIAVVLVMAGFAVISKAFAAG
                     GRTPQFEMASGVLILLIGAFLLWRAATRRSHPQDGAEARNGRALAFVTGLVPCPLTTF
                     IMSYALARGMLAAGLAVTAAMAVGMVVTIAAVAVIATLSRDRLMAFFADTKNIRSWLG
                     RALETGGALLVFLFGAWVLIGAQ"
     gene            complement(4412..4705)
                     /locus_tag="AC16Y7_02850"
     CDS             complement(4412..4705)
                     /locus_tag="AC16Y7_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708349.1"
                     /translation="MKTLRLSLVLCGALGLALLPARVSAFEIQGQDAELPKSAAEYHG
                     LTPTYTMPRFEGSSLAMPYSSSNSLEGHISDYGNSITIPGPGVDQAAPAWAYR"
     gene            4894..>5924
                     /locus_tag="AC16Y7_02855"
     CDS             4894..>5924
                     /locus_tag="AC16Y7_02855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5708350.1"
                     /translation="MAPRILYVVTEDWYFLSHRLPMARAARDAGYEVHVAARVKDGRA
                     GIEAEGFVLHALDWSRGSLSLGDSLSAIRALRQLFASLKPDIIHNIAVKPAVLGSAAS
                     LGLKSVVVNSLTGVGSLFIGEAQVSGLTRRAVRFALGRLLQRDTSWTVVQNPDDRAFV
                     LELGLTPEHIVLIRGSGVDTEHLAPLPEPPAPVTAAYVGRMLADKGVPTLIDAVKRLR
                     DRGVEMRLLLAGDCDTENPGSLTPEQLREFVGALGIQWLGHVSDIREVWRQAHFAVLA
                     SRREGLPKSLLEAAACGRAMVATDAPGCREVAIEGKTALTVPVDDVEALAGAMARLAE
                     DAALRERFG"
BASE COUNT         1137 a         1859 c         1930 g          998 t
ORIGIN      
        1 attcagcacc tatatcgtct ctggcggcgg tatcgaattc gtgcgcaatt tctcggagcc
       61 ggtctatggt attccgcccg cgcaggtggt cggctcaagc atcaagaccg aatacaaggt
      121 tgtcgacggc aaaccgaaga tcatgcgctt gccggaagtg aacttcatcg acgacaaggc
      181 tggtaagccc gtcggcatca accagcacat cggcaagcgg ccgatctttg cgtccggtaa
      241 ttccgacggc gacttcgaga tgctggagtg gacaacggcg ggcgatggtc cgcgttttgg
      301 cctgatcgtg catcacaccg atggcgagcg cgaatacgcc tatgaccgcg agtcccatgt
      361 gggccgcctc gacaaggcgc ttgatgcggc cgcggacaac ggctgggtcg tgatcgacat
      421 gaagaacgac tggaaggaga tatttccccc ggcggactag gcccaagagg cgtcctcgcg
      481 gtggaaaaga tcgcaccgcc ggttgacctc gcggcggccc gagcgtatgg gccgttccca
      541 tcaatcatgg gacgtttcgt ttcctttgca gacaatcggg gacgaatcgg ccatgcctga
      601 tagggcccag atatggccaa gggggcgaag caaccgaggc cctccttttt caggagtgag
      661 agaaatcaaa tgatatcaaa aaccgaactc atccaacgta tcgccgatga gctagagatg
      721 accaagcggg acgtgaagaa cgtcctcgag actctggcga ccgttggcta caaggaaatg
      781 aagaagaccg gcgagttcat gcttccgggc ttcgccaaat tcgtggtcgt caagaagccg
      841 gcaaccaagg cacgcaaggg catcaatccc ttcaccaagg agccgaccgt cttcaaggca
      901 aaaccggcgc gcaagattgt taaggcccgt ccggtcaagg ccgcgaagga cgccgtctag
      961 cacggctccc ggcgctcaaa tgtgtgggcg agaggaaacg tatccccgcc tttcgaggcg
     1021 gggactacgc cttgctttgc ctctcggctt gcgggtccgg atttgcgggc gtcttcgcgc
     1081 ttccctttgc ggccccgcgc gaagtccgag taaggtagga ccgtgacccg gcgccgcaaa
     1141 tcgccttcta agaccaggaa acagccgagg aagcaggcaa gacagcctgc gccggcggcg
     1201 aagcctgagg ccgccgcgcc ggcccgctca tcctcgtggc gactctggat cgtggccggc
     1261 attgccgcag ccgccgtcct ggcggccggg ctgctgtggc agagcctgga tcgaaaacct
     1321 accggcagcg ccgacacgtc acccgaaacg ctcgccttcg tcggcagtga aacttgtatc
     1381 ggttgccacc aggaggccgg caagctttgg cacggctctc agcatgctca cgcgatggcg
     1441 catgcgacgg cgaagactgt gctcggcgat ttcgacgacg cgacattcgt ctatggggac
     1501 gtgacgtcgc gcttcttcaa gaaggacggc aagttcttcg tcgagaccga cgggccggac
     1561 ggcgacctcg aaacgttcga ggtgaagtac acgttcgggc tggagccctt acagcaatat
     1621 ctgatcgagt tcccggacgg gcgcgtgcag tctctgacca tcgcctggga cagccggccg
     1681 aaggaagagg gcgggcaacg ctggtaccat ctttatcacg acgagatcat cgcgcacgac
     1741 gacccgcttc actggaccaa gctccagcag aactggaact ttatgtgcgc cgagtgccat
     1801 tcgacgggag tggcaaagaa ctacgatccg gcgacggaca gcttcgacac cacctggaag
     1861 gaaatcagtg tcggatgcga ggcgtgtcac ggcgagggct atgcgcatgt ggcctgggcg
     1921 aacacatggt cgccgttcgc caagaaggac aaggcgaaag gattattcgc cgcgttcgac
     1981 gagcgcacga acgtcacctg gacgcaaaat aagaagaccg gccagccggt gcgggaccca
     2041 cggcccacgc atttgcgcaa agaggttgag atgtgcggac gctgccatgc gcggcgcggg
     2101 cagatctcgg aggccttcgt gcctgggcgt ccgctttccg acactcatac ggtggggctt
     2161 atcacccgcg acctctatca accggacggt cagaagcgcg gcgaggtcta taactacggc
     2221 tcattcaagc agagcaagat gtttgccgca ggggtgacgt gcagcgactg ccacgagccg
     2281 cacagtgcga gtttgcgtgc caaaggcgac caagtctgct tgcagtgcca tgcgccacac
     2341 tatgcggaga caagtcatac gcaccatgag ggcgagaacg cgccgggctg cgtcgcctgc
     2401 cacatgcccg cccgcgagtt catggtgatc gacacgcggc acgatcatag cttcagggtg
     2461 ccgcgtcccg atctgtccgt gaccttgggc gtggacaatg cctgtaccga ttgccacacg
     2521 ggcaagtcgt ctgaatgggc ggcagccgcc atcgaggact ggttcgggcc cgagcgcaaa
     2581 gggttccaga catttgctcc tgctttccaa gcggcgtggg aaggcgcagc ggataccgag
     2641 gccttgcttc aggcggtggc cacggaccca aagacgccgg ccttcgcccg cgccagcgcc
     2701 ttccaagaac tcaccgcttt cccgtctgtg gagaccgcta gagcggtgcg cgaagggctc
     2761 gcagatcctg acccgttggt gcggctcgcc gcgctcgatc atttggaaac ggcgcagccc
     2821 gggcggcttt ggcccatcgt gagcccgctt ctcgacgatc cggtgcgcgg tgtgcggatc
     2881 aaagccgcgc ttctgctcgc cggcacgcca cagcaagctc tccagcctgc ggatcgcgcg
     2941 cgactcgcca aggccgagaa ggaatttatg gccgcgcagc gcctcaacgc ggaccggccc
     3001 gaggggcgcg cggtgctcgg ccgctactat gcgcggggcg gcaatgtggc gaagtcggag
     3061 gaggagtatc gcgcggcgtt gcgcttgagc ccgcacttcg cgccggctgc cgtcaacctc
     3121 gccgatctct accggcagag cggccgcgac gaggacggcg ttgccgtcct gcgggaggcg
     3181 ttgaaactgt cgccggacgc cggcggtcta catcacgcgc ttggtctcgc cctggtgcgt
     3241 ctcaagaagt cggatgaggc actcgaaagc ttgcgtctcg ccacggagct cgacccggac
     3301 cgcgcgcgct acgcttatgt ctatgccgtc gctctggact cgcaaggacg gcggcccgag
     3361 gcaatcgagg tgttgaagga gagccttaag cgccatcaaa gcgatcgcca aactcttggt
     3421 gggttgatca acttcacccg cgccgacggc gataccgcag ccgcgttgcg ctacacggag
     3481 cagctcctaa agctcgaacc ggaaaatccg aacttgaagc agctgatcaa agctctgaaa
     3541 gatgcggacg accaaggttc ggacgatcaa ggcgcgcagt agctcacgcc gcttattgcg
     3601 cgccgatcag cacccacgcg ccgaacaaga agacgagcaa cgcgccgccg gtctctagcg
     3661 cacgtcccag ccagctccga atgtttttgg tgtccgcgaa gaacgccatg aggcggtcgc
     3721 gcgataaggt ggcgatcacc gccacagcgg cgatggtcac gaccatcccg accgccatgg
     3781 ccgcggtcac ggcgagcccg gcggcgagca tcccgcgcgc cagcgcatag ctcataatga
     3841 aggtcgtcag cgggcagggc acgagccccg tgacgaaggc cagcgcccgg ccattgcgcg
     3901 cctcggcgcc gtcttgcgga tgcgagcggc gcgtcgccgc gcgccacagc agaaatgcgc
     3961 cgatcagcag gatcagaacg ccgctggcca tctcgaattg cggcgtgcgt ccgccggccg
     4021 cgaaggcttt gctgatgacg gcgaagccgg ccatgaccag cacgacggcg atccctacat
     4081 gcgtcagggc gaggatcgcg ccattggcga tgctcgatag caggctgccc ttttggccga
     4141 gatggtagga gaacagaacg gtcttgccgt gtccgggcat catggcgtgg agaaagccga
     4201 acaggatggc gccagccatc acggccggaa gcgccaaagc gccgccttgg gcgacttcgc
     4261 caagaccgtc cgccatgccc tcatagagcc agcgctgaag cgcgatgatc tcctgcatgg
     4321 gctcgggtgt tacaggcggc cgcgagcgcg agcaagccgg cgcggcccaa ggctcacgga
     4381 gaagtcatcg aaagaaagag tggttcggcg tctagcggta ggcccaagcc ggcgccgcct
     4441 ggtccacgcc cggcccggga atcgtgatgg aattcccgta atcggagatg tggccttcga
     4501 ggctgtttga actgctatat ggcatggcca atgaactgcc ttcgaaccga ggcatggtgt
     4561 atgtcggggt cagaccgtga tattccgccg cgcttttcgg cagttcggcg tcctggcctt
     4621 gaatctcgaa cgcgctcacg cgggccggca gcaatgccag tccgagggcg ccacacagca
     4681 ccagactaag acgaagcgtt ttcatcagtt ccctcaatgc cgcttttcgc agattaggcc
     4741 catcctactg cgatttgctg gggaaggcca gccgggttca tcgttgtccc cggcccccgt
     4801 ggccgccccg caacaggcga tcgtccccct agaatcccct gcaaaccggc aaaattgcgg
     4861 caggcgccgc cagcagataa aagcaaccgc gatatggccc cgcgcatcct ctacgtcgtc
     4921 accgaggact ggtatttcct gtcgcaccgg ctgcccatgg cccgggcggc cagggacgcc
     4981 ggctatgagg tccacgttgc ggcccgggtc aaagatgggc gggccggcat cgaagccgaa
     5041 ggcttcgtgc ttcacgcgct cgattggagc cgcggcagcc tctcgctggg ggatagcctt
     5101 tcggccattc gcgcgcttcg gcagctgttt gccagcctga agcccgacat catccacaac
     5161 attgcggtga agccagcggt gcttggctcg gcggcgagcc ttggtctgaa gtctgtcgtg
     5221 gtcaatagcc tgaccggggt cggatcgttg ttcatcggcg aggcgcaagt gagcgggctg
     5281 acgcgccgtg ccgtccgctt cgcgcttggc cgcctgttgc agcgggacac aagctggacc
     5341 gtggtgcaga acccggacga tcgcgccttc gtcctcgagc ttggcctgac gccggagcat
     5401 atcgtgctga tccggggctc tggcgtcgac accgagcacc tggcgccgct gcccgaaccg
     5461 ccagcgccgg tcacggccgc ttatgttggg cgcatgcttg ccgacaaggg cgtgccgacg
     5521 ctcatcgacg ccgtcaagcg ccttcgtgac cgtggcgtcg aaatgcggct cctgctggcc
     5581 ggcgattgcg acaccgagaa tccgggttct ctcacgcccg aacagttgcg cgagttcgtc
     5641 ggcgctttgg gcatccaatg gctagggcat gtcagcgaca tccgcgaggt ctggcgccag
     5701 gcgcatttcg ccgtgctcgc ctcgcggcgc gaagggctgc caaagagtct gctggaagcg
     5761 gcggcctgcg gccgggccat ggtggcgacg gacgcgcccg gctgccgcga ggttgccatc
     5821 gagggcaaga cggcgctcac cgtccccgtg gacgacgtgg aagccttggc cggggccatg
     5881 gctcgcctgg cagaagatgc cgctttgcgg gagaggttcg gcag
//
LOCUS       DBNHVU010000075         3612 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_772712, whole genome shotgun sequence.
ACCESSION   DBNHVU010000075 DBNHVU010000000
VERSION     DBNHVU010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3612
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_772712"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..635
                     /locus_tag="AC16Y7_02860"
     CDS             141..635
                     /locus_tag="AC16Y7_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HIR5708351.1"
                     /translation="MPSDRATLFARLEELGIASKTVEHAPMFTVEQSQDLRATIPGGH
                     TKNLFLKDKDGRLALVIAKDDTKVDLKALAKQLDLGRFSFGKPDLLMDVLGVEPGSVT
                     AFALINETSRRLSAVVVDKALLRFAEVNCHPLINSATTRVATGDLIRFIEACGHTPHL
                     LVLT"
     gene            734..1645
                     /gene="trxA"
                     /locus_tag="AC16Y7_02865"
     CDS             734..1645
                     /gene="trxA"
                     /locus_tag="AC16Y7_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR5708352.1"
                     /translation="MSVETPIFGEGAGEPGADLIKNTTTKDFMADVIDASRERPVLVD
                     FWAPWCEPCRQLTPVLEKVVRASKGTIRLVKLNIDEHPEIPGQMGVQSIPAVFAFKDG
                     QPVDGFMGALPESQINAFVARLVGENTEETADLEAAEAALEAGDVNAAAQAFAEILQG
                     DAENAEAAAGLAKCYIKTGDLARAEQTLALVPPAKAESAPVASARAALELAKKAGDTG
                     DVDSLRAKVAADPSDPQARFDLALALNASGDRQGALDELLTIVAKNRAWDDDAARKQL
                     VQFFEAWGATDPATTSGRQKLSSLLFS"
     gene            1661..2338
                     /locus_tag="AC16Y7_02870"
     CDS             1661..2338
                     /locus_tag="AC16Y7_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR5708353.1"
                     /translation="MPDRYRNASDMPDMLPVFPLRGAILLPRATLTLNVFEPRYLAMI
                     EHALANERLIGVVQPAPDTGSDESPQGKNVPLRNIGCAGRITAFNENEDDHLVVTLTG
                     VARFQLGGEVSSDMSFRLCEISFSSYADDFIAGLGEDDVDRVGLLETLRCYLEAYDLN
                     ADWDRINSADNERLVNTLSILSPYGAEEKQALLEAVSLQDRAEALVALAEMELATRDD
                     GSGTSIQ"
     gene            2335..2550
                     /locus_tag="AC16Y7_02875"
     CDS             2335..2550
                     /locus_tag="AC16Y7_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HIR5708354.1"
                     /translation="MTREDKDTTPAGDRPVDPKLLEVLVCPLTKTSLDYDAKRQELIS
                     RAAGLAFPIRDGIPIMLPEEARKLDES"
     gene            complement(2547..3416)
                     /gene="gluQRS"
                     /locus_tag="AC16Y7_02880"
     CDS             complement(2547..3416)
                     /gene="gluQRS"
                     /locus_tag="AC16Y7_02880"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HIR5708355.1"
                     /translation="MSGFVTRFAPSPTGLLHLGPGFSAMTAWEAAKSAGGTCLLRIED
                     TDATRVRPEYEAAIYEDLAWLGLTWPEPVWRQSDRLDVYAAALDRLGRLGLTYPCRCT
                     RADIRAAVAAPQEGAGPGSAVYPGTCRGRVLAEAGQGEAIRLDLSRALALLDGPALTW
                     TETGPAHVGTHALDPGAMRREIGDVVLARKDIGAAAYHLSVVVDDAAQGITHVVRGED
                     LLDATPIHRLLQALLELPVPVWHHHALVRDAGGKRLAKRDDARAIREYRKAGLTPEDV
                     RRLAHTLRADEPR"
BASE COUNT          684 a         1175 c         1146 g          607 t
ORIGIN      
        1 atttcacgct tcagggtggt catttgcctt gtagtggatc gcgcctgaac tgtctacttc
       61 agccgccttc cccaaaacta tgaccccttc aggggcccat caagggcacc ccccattagc
      121 cgaagcccac gccgaaagca atgccatccg atcgcgccac gctgtttgcc cggctggaag
      181 agctgggcat tgcctccaag acggtcgagc atgcgcccat gttcaccgtc gagcagtccc
      241 aagatttgcg ggccacgatt ccaggcggac acaccaaaaa tctattcctc aaggacaagg
      301 acggccgcct ggcgctggtg atcgccaagg acgacaccaa agtcgacctc aaggccctgg
      361 ccaagcaact cgaccttggc cgtttcagtt tcggcaagcc ggatctctta atggatgtcc
      421 ttggcgtgga gccgggctcg gtcaccgcgt ttgccctgat caacgagacc tcacgccggc
      481 tgtccgcggt agtggtggac aaagccttgc tgcgcttcgc cgaggtcaac tgtcacccgc
      541 tgatcaatag cgcaacgaca cgtgtggcaa ccggggacct gatccgcttc atcgaggcgt
      601 gcgggcacac accccacctc ttggtcttga cttgagcggc tggacgcggt tgccaaatgg
      661 cggcatgcgg cccatcttct atcgtgaaag cggcacacca tttgccggtg ccgcaccgca
      721 cggaaagtga agcatgagcg tagagacacc gatattcggc gagggcgcgg gcgaacccgg
      781 cgccgacctg atcaagaaca ccacgaccaa ggactttatg gccgacgtga tcgatgcctc
      841 acgtgagcgg cccgtcttgg tggacttctg ggcaccgtgg tgcgaaccgt gccgtcagtt
      901 gacccccgtc ctggagaagg tggtgcgggc gtccaagggc accatccggt tggtgaagct
      961 caacatcgac gagcacccgg agattccagg ccagatgggc gtgcaatcga ttcccgccgt
     1021 gttcgccttc aaggatggcc aaccggtgga cggctttatg ggcgcgttgc cggaatcgca
     1081 aatcaatgcc ttcgttgcac ggctggtcgg cgagaacacc gaggagaccg cggatctcga
     1141 ggcggcagaa gcggcgctcg aagccggcga cgtaaatgcc gcggcgcagg cgtttgccga
     1201 gatcctgcaa ggcgacgcgg agaatgcaga agccgccgcc ggccttgcca agtgctacat
     1261 caagacaggc gatctggcgc gcgccgagca gacactggcg ctcgtcccgc ccgcgaaggc
     1321 cgagagcgcg ccagtcgcaa gcgcgcgagc cgcgctcgaa ctcgccaaga aagccggtga
     1381 cacaggcgac gtggacagcc ttcgtgcgaa ggtcgccgcg gacccgagcg acccgcaagc
     1441 caggttcgat ctcgcgctcg cactgaacgc gagcggcgac cgtcagggtg cgctcgatga
     1501 gctgctgacc atcgtcgcga agaaccgcgc gtgggatgac gacgccgccc gaaaacagct
     1561 tgtgcagttt tttgaggcgt ggggcgcgac ggaccccgcc acgacatcgg ggcgtcaaaa
     1621 gctgtcgtcc ttgttattct cgtaaacgcc gaggagcgtc gtgcctgatc gctatcgcaa
     1681 cgcctcggac atgccggaca tgttgccggt gttccctttg cggggcgcaa ttctgctgcc
     1741 acgggcaacg ttgaccttga acgtgttcga gccgcgctat ctggcgatga tcgaacatgc
     1801 cttggccaac gagcggttga tcggcgtggt gcagccagcc cccgataccg gcagcgatga
     1861 gtctccccag ggcaaaaacg ttcccttgcg caacattggt tgtgccggcc gcatcactgc
     1921 gttcaatgag aacgaggatg atcaccttgt ggtcacgttg accggtgtcg cgcgctttca
     1981 gcttggcggg gaagtctcat cggacatgtc gttccgtctg tgtgagatct cattttcatc
     2041 ctatgcggat gacttcatcg ctggtctcgg cgaggatgat gtcgaccgtg tcggcctgct
     2101 cgagacgctt cgttgctatc ttgaagcata cgatctcaat gccgactggg atcgcatcaa
     2161 cagcgcggac aatgagcggc tggtgaacac gctttcgatt ctcagcccct acggtgccga
     2221 ggaaaaacag gcactgttgg aggccgtgag ccttcaagac cgcgccgagg ctttggtggc
     2281 actcgcggag atggaactcg ccacgcgcga tgacggttcg ggaacctcga tacaatgacg
     2341 cgcgaagaca aagacacaac gccggcgggc gaccggcccg tcgatccgaa gcttctcgaa
     2401 gtcctcgtct gtccgctgac aaagacgtct ctcgactacg acgcgaagcg gcaggagcta
     2461 atcagccgcg ctgcgggact cgcttttccc attcgtgacg gcatcccgat catgttgccg
     2521 gaagaagcgc gcaagctcga cgagagctaa cgcggctcgt cggcgcgcag cgtgtgcgcc
     2581 aggcgccgca catcctccgg cgtgagcccc gccttacgat attcgcggat tgcgcgcgcg
     2641 tcgtcgcgct tggcaaggcg cttgccgccc gcatcgcgca cgagcgcgtg gtgatgccag
     2701 accggcaccg gtagttcgag cagcgcctgc aagagccggt gaatgggcgt ggcgtccaaa
     2761 agatcctcgc cgcggacaac atgggtgatc ccttgcgcgg catcgtccac caccaccgag
     2821 agatgatagg cggccgcgcc gatgtccttg cgcgccagaa ccacgtcgcc gatctcgcgc
     2881 cgcatggccc ccgggtccag cgcgtgtgtc ccgacatgcg ccgggccagt ctcagtccaa
     2941 gtcagtgccg gaccgtcaag gagcgccaac gcgcggctga gatcgagccg gatcgcctcg
     3001 ccctgcccgg cctcggcgag cacgcggccg cggcacgtgc cgggatagac cgcgctcccg
     3061 gggccagcgc cttcttgcgg cgcggcgacc gcggcgcgga tatcggcgcg ggtgcagcgg
     3121 caggggtaag tcagcccaag acggccaagc cggtcgagcg cagccgcata gacgtcgagc
     3181 cgatcggatt gccgccacac aggctccggc catgtcagcc ccagccacgc caagtcttcg
     3241 tagatcgccg cctcgtattc gggccgcacg cgcgtcgcgt cggtatcctc gatccgcaag
     3301 aggcacgtcc cgcccgcgga cttcgccgcc tcccaggcgg tcatggcgga aaagccagga
     3361 ccgagatgca gcagccccgt cggcgagggg gcaaaacgcg tgacgaaacc ggacatcgtg
     3421 gggacgtgcg ccacgcgcta cagggcctcg cccttcatca gccgcggcac gtcgccggtt
     3481 ccgccggccg cctcacgcac taagaagcgc tttaggcccg gcgaccggtc gacaatcccc
     3541 agcccgagat cgcggatggc acgaagcggc gcgttgtcgg tggagaacag gcggttcatc
     3601 gcgtccatca cc
//
LOCUS       DBNHVU010000076         7463 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1887251, whole genome shotgun sequence.
ACCESSION   DBNHVU010000076 DBNHVU010000000
VERSION     DBNHVU010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7463)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7463)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7463
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1887251"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1020
                     /gene="dapD"
                     /locus_tag="AC16Y7_02885"
     CDS             178..1020
                     /gene="dapD"
                     /locus_tag="AC16Y7_02885"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HIR5708356.1"
                     /translation="MSHDHLKNFIDGAFQHAAEITPNSKGVVRDAVEEVLNLLDKGEL
                     RVAEKTNGEWAVNQWVKKAILLSFRLEDPHRMEGGPDDSSWWDKVPVKFEKWKAKDFK
                     EAGFRAVPGCIVRHSAYISPGVVLMPCFVNLGARVDSGAMIDTWATVGSCAQIGKNVH
                     LSGGAGIGGVLEPLQANPVIIEDNCFIGARAEVAEGVIVGEGSVLSMGVYIGQSTKIV
                     DRATGFVHQGRVPPYSVVVSGSMPGKPLPDGSPGPSLYCAVIVKTVDEQTRSKTSINE
                     LLRD"
     gene            1139..1588
                     /locus_tag="AC16Y7_02890"
     CDS             1139..1588
                     /locus_tag="AC16Y7_02890"
                     /inference="COORDINATES: protein motif:HMM:NF017470.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HIR5708357.1"
                     /translation="MMDLQYLFASFDGRLNRAKWWVGVVILAIISLAFGLIIELIFGG
                     GFFGGFLATLVALVLFFPTYALCAKRFQDRDKPGKTALYGLVPSLLAGLFDAWGMTGT
                     PAELNGLGWLCMLVNLGVGLWFLIDLGILKGTPGPNNYGGDPLTALT"
     gene            1710..2903
                     /gene="dapE"
                     /locus_tag="AC16Y7_02895"
     CDS             1710..2903
                     /gene="dapE"
                     /locus_tag="AC16Y7_02895"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HIR5708358.1"
                     /translation="MTTPGERAVALAQLLIRCASVTPKDAGALDVLIEPLTKARFACE
                     RLTFSEDGTPPVDNLLARIGEGPPHLCFAGHTDVVPPGDEALWSHPPFAAEIADGVLF
                     GRGASDMKGAIACFASAALDYAKAKNREIDGTISLLITGDEEGPSINGTRKVLKWMEH
                     KGLKPDHCIVGEPSNTDKLGETIKIGRRGSLNGRLKVAGQQGHVAYPHLAANPVKGII
                     AALTRIYDEPLDYGSAHFSPSNLEVTSIDVDNPATNVIPASAEAKFNIRFNDRHGPDS
                     LEKMLREQVGAALNDTELTFTLEFDPPGDVFVTEPGPLDALLSESVHEVTGLTPALST
                     AGGTSDARFIKDYCPVVEFGLTTQTIHKTDENAKVSDLEQLTAIYRRFIERYFEAFAG
                     GPDGR"
     gene            2893..3723
                     /gene="murI"
                     /locus_tag="AC16Y7_02900"
     CDS             2893..3723
                     /gene="murI"
                     /locus_tag="AC16Y7_02900"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIR5708359.1"
                     /translation="MGGDGRPIGVFDSGMGGLTVLRALAVRLPHERFVYLGDTARLPY
                     GTKSAETVQAYALQATQLLVGEGVKMVVIACNTASAVALAVLQESWDPVPVIGVIEPG
                     AAAGVAATKNKRIAVLATESTVQGRAYARAIQRLMADADVVQQPCQVFVALAEEGWAE
                     TPATLATAKHYLEPLFDGDDAPDTLVLGCTHFPVLANAIRRTVGTNIALVDSAETTAA
                     AVADALEDAGAASAGPASNASIRFFATDSPERFARVGAIFLGREIAVDDVELVDLRKA
                     "
     gene            complement(3720..5351)
                     /gene="murJ"
                     /locus_tag="AC16Y7_02905"
     CDS             complement(3720..5351)
                     /gene="murJ"
                     /locus_tag="AC16Y7_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR5708360.1"
                     /translation="MIRRIATVGGLTLVSRLTGFIRDVVMAAILGAGPIADAFFVAFR
                     LPNHFRAIFAEGAFAAAFVPAYARTLEEAGLPAAKLFADQIAAALVTINLVLLAIALL
                     FTPEVVGLLAPGLVGDPERYDLAVALTRITFPYLALVSLETLFAGVLNANNRFAAAAS
                     APVLLNLSVIATLLLTPYFPDAGHAAAWGVLIAGVAQAILLGFDAEAHGFGIRLRMPK
                     LDEPTRKFLKALGPAILGAGGVQLALFADTLIATFLPTGALSALYYADRINQLPIGVV
                     GIAVGVVLLPEMSRRLAAGDEAGAASAQARGIQLALLLTVPCAAAAVAIPDLIMRALF
                     ARGAFTAADALAAGNTLAAYSIGLIPFVLLRSFTAPFYARRDTLTPVKAALLAATVNI
                     GLKVVLMGPLAQVGLALATSIGAWINLSLLAYFARRQGFAVSGTAIGGPVAKLLIVGV
                     VFAGALFAGAYGLHQALASMTHFRDETALAILVIGGGVLYFALVAVVLGRNWFMGLLK
                     DVSTAADTDAPAELDEPDPTDDSAALPDSEPPPKG"
     gene            complement(5403..6140)
                     /gene="truA"
                     /locus_tag="AC16Y7_02910"
     CDS             complement(5403..6140)
                     /gene="truA"
                     /locus_tag="AC16Y7_02910"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR5708361.1"
                     /translation="MPRYKLTLEYDGTPFVGWQRQDGQMSVQGRLAQAIKDFSGEDAM
                     PGGAGRTDAGVHAIGQVAHVDLERDWAPAKIRDAINAQLRPEPISVLDCEQVPDGFDA
                     RFSAKARHYLYRVVDRRSPLALERGRAWGVFKPLNAKAMHDAAQGLIGHHDFTTFRST
                     ECQAASPEKTLDRLEVSRHGEIVRIEASARSFLHNQVRSMVGSLKLVGEGRWTSGDLK
                     KALETKDRAACGPVAPPQGLYLVKVDY"
     gene            complement(6143..7069)
                     /gene="fmt"
                     /locus_tag="AC16Y7_02915"
     CDS             complement(6143..7069)
                     /gene="fmt"
                     /locus_tag="AC16Y7_02915"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR5708362.1"
                     /translation="MRIIFMGTPDFAVPALQALLKAGHEVAAVYTQPPRAAGRGMALR
                     KSPVHEAAEAAGLEVRVPSDLKSPAEQKRFAALEAEAAVVVAYGLILPKQVLNATRRG
                     LFNIHASLLPRWRGAAPINRAIMAGDAESGVCIMRITEGLDEGPVCLMERESIGPDMT
                     AGQLHDALSELGASMIVEALAALESGTLQCLAQDDANATYAAKIKNADTRIDWSLAAK
                     VVHNHIRGLSPYPSAWFEINLGGKRERVKVLDSKLADDNAPAGMLLDDALTVACGEGA
                     VRLTEVQRAGKKPMSADSFLRGIDLPARTVFD"
     gene            complement(7118..>7463)
                     /gene="def"
                     /locus_tag="AC16Y7_02920"
     CDS             complement(7118..>7463)
                     /gene="def"
                     /locus_tag="AC16Y7_02920"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR5708363.1"
                     /translation="LIVMDATGDDEPKDPIVMVNPKILDRSEELRVHEEGCLSIPEVT
                     AEVERPAVSRVAYIDREGKRQERVLEGILSTIVQHEVDHLNGVLFIDYLSRLKRDMIV
                     KKFTKQKRAEAM"
BASE COUNT         1340 a         2394 c         2478 g         1251 t
ORIGIN      
        1 ggccggcccg agcatcagag ccacgagacg tggtccatac tgcccgatta cattcatcac
       61 cgtaccgagg cgcttgcgcc tttcctcacc gccatcgcga gcgctcttgc cgaagacgcc
      121 gagagccccg cgacgcgctt gccccaagca cagttctgcc tgacctaacg gagtcccatg
      181 tctcacgatc atctcaagaa tttcatcgac ggcgcttttc agcacgcagc agagatcaca
      241 ccgaacagca aaggcgtcgt gcgcgacgca gtcgaggagg tcctgaacct gctcgacaag
      301 ggcgagctgc gcgtggccga gaagacgaac ggcgagtggg ccgtgaacca atgggtgaag
      361 aaggcgattt tgctgtcgtt ccgcttggag gatccgcacc ggatggaggg cgggccggac
      421 gactcgtcct ggtgggacaa agttccggtc aagttcgaga agtggaaggc gaaggacttt
      481 aaggaagcag gcttccgcgc cgtgccgggc tgcattgtgc gccactcggc ctatatctcg
      541 cccggggtcg tgctcatgcc ttgcttcgtc aatctcgggg cgcgcgtgga tagcggcgcc
      601 atgatcgaca cctgggctac ggtcggctct tgcgcgcaga tcggcaagaa cgtgcacctg
      661 tcgggcggcg ccggcattgg cggcgtgctg gagcccttgc aggcgaaccc cgtgatcatc
      721 gaggacaatt gcttcatcgg cgcgcgcgcc gaggtggccg aaggcgtgat cgtcggcgag
      781 ggctccgtgc tctccatggg cgtctatatc ggccagtcca ccaagatcgt cgaccgcgcc
      841 acgggcttcg tgcaccaggg ccgcgtgccg ccctactccg tggtggtgtc cggctcgatg
      901 ccgggcaaac cactgccgga cggctcgcct ggcccctcgc tctactgcgc cgtcatcgtc
      961 aagacggtgg acgagcagac ccgctccaag acctccatca acgagctgtt gcgcgactga
     1021 gatatcccgt taacccagcg cgggatttca cggcgttggg ttcatatccc tcttcgcaag
     1081 acggccgttc ggccttacaa actcagaggc tgagtcgcga aaggccgtcc aggggggaat
     1141 gatggaccta caatatctct tcgcttcgtt tgacggacgc ctcaaccggg ccaaatggtg
     1201 ggtcggcgtg gtcattctcg ccatcatcag cctggctttc gggctgatca tcgagcttat
     1261 tttcggcggg ggctttttcg gcggttttct ggcaaccctc gtcgcactcg tcctgttctt
     1321 cccgacctat gccttgtgcg cgaagcgctt ccaggaccgg gacaagccgg gcaagaccgc
     1381 gctctacggc ttggtgccct cgttgctggc cggcttgttc gacgcctggg gcatgacggg
     1441 cacacctgcc gaactcaatg ggcttggctg gctttgcatg ctggtcaatc tgggcgtggg
     1501 cctttggttc ctcatcgatt tgggcattct gaagggcacg ccggggccga acaattatgg
     1561 cggcgacccg ctgacggccc tcacctagtc ctcaaaagct tcgctgatgc ataaggccga
     1621 gcaggcctcg ccgcttgcca gggggccggg gccccgtcat gttctccggg cgcggcgctc
     1681 cgtcgcgccg gaacccaagg gaccaatcca tgaccacgcc cggcgaaagg gccgtagcgc
     1741 tcgcacaact cttgatccgc tgcgccagcg tcacgccaaa ggacgcgggc gccttggacg
     1801 ttctgatcga gccgctgacg aaggcgcggt tcgcctgcga acgcctcacc ttcagcgaag
     1861 acggcacgcc gccggtggac aatctcctgg cgcggatcgg cgagggcccg ccgcatctgt
     1921 gcttcgccgg ccatacggac gtggtgccgc cgggggacga ggccctgtgg agccatccgc
     1981 ccttcgccgc ggagatcgcc gacggggtgc tcttcggccg cggcgcgtct gacatgaagg
     2041 gcgcaatcgc ttgctttgcg tccgcggcgc tcgactacgc caaggcgaag aaccgcgaga
     2101 tcgacggcac gatcagcctg ctcatcaccg gcgatgaaga aggcccgtcc atcaacggca
     2161 cgcggaaagt gctcaagtgg atggagcata agggcctgaa gcccgaccac tgcatcgtcg
     2221 gcgagccctc caataccgac aagcttggcg agacgattaa gatcgggcgc cgcggcagcc
     2281 tcaacggccg gctgaaggtc gcgggccagc aagggcacgt ggcgtatccg catcttgccg
     2341 ccaatccggt gaaaggcatc atcgcggcgc tgaccaggat ttacgacgaa ccgctcgact
     2401 acggtagcgc gcatttctcg ccgtctaatc ttgaggtcac cagcatcgac gtggataacc
     2461 cggcgacgaa cgtgatcccg gcaagcgcgg aagcgaagtt caatatccgc ttcaacgacc
     2521 ggcatggacc ggattcgctg gagaagatgc tgcgggagca agtgggggcg gccctcaacg
     2581 acaccgaact caccttcacg ctggagttcg atccgcctgg cgatgtgttt gtcaccgagc
     2641 cgggtccgct cgacgctctc ttgagcgagt cagtgcacga ggtcacggga ctcacgcccg
     2701 cgctgtcaac ggccggcggc acctcggacg cgcgctttat caaggactac tgccccgtcg
     2761 tggagttcgg gcttactacc cagaccatcc acaagacgga cgagaacgcc aaggtcagcg
     2821 acctcgagca actgaccgcg atctatcggc gcttcatcga gcgctatttc gaggcgtttg
     2881 ccgggggacc cgatgggcgg tgacggccgg ccgatcgggg tcttcgactc cggcatgggc
     2941 ggcttgacgg tgctacgggc ccttgccgta cgcctcccgc atgagcgctt cgtctatctc
     3001 ggcgatacgg cgcggctgcc ctacggcacc aagagcgcgg aaaccgtcca ggcttacgcg
     3061 ctgcaagcca cgcagctcct cgtcggcgaa ggcgtgaaga tggtggtgat cgcctgcaac
     3121 accgcctctg ccgtggcact tgccgtgctg caggagtcat gggacccggt gcccgtgatc
     3181 ggggtgattg agccgggcgc cgcggcggga gtcgctgcga caaagaacaa gcgcattgcc
     3241 gtactcgcga ccgaaagcac cgtgcaggga cgcgcctatg cgcgggccat tcagcggctg
     3301 atggcggacg cggacgtcgt ccagcagccg tgtcaggttt ttgtggcgct cgccgaagaa
     3361 ggctgggccg aaacgccggc gacactcgcc actgctaagc attatctcga gccgctattc
     3421 gatggggacg acgcccccga cacgctggtg cttggctgca cgcactttcc cgtgctggcg
     3481 aacgcgatcc gccgcaccgt ggggacaaac atcgcgctcg tggattcggc ggagaccacg
     3541 gcggcggccg tcgcagacgc acttgaggac gcaggtgcgg cttccgcagg gcccgcgagc
     3601 aacgcctcga tccgtttctt cgcgacggat tcgccggaac ggtttgcgcg cgtcggcgcc
     3661 atcttcttgg gccgtgagat cgccgtggac gatgtcgagc ttgtggactt gcgaaaggct
     3721 tagcctttgg gcggcggctc ggagtccggc agcgcggcgg aatcgtccgt gggatcaggc
     3781 tcgtcgagtt cggcgggcgc atccgtgtcg gcggcagtcg acacatcctt caacagcccc
     3841 atgaaccaat tccggccaag gaccaccgcg acgagcgcga agtagagcac gccgccgccg
     3901 atcacgagaa tggccagcgc cgtctcgtcg cggaaatggg tcatcgaggc aagcgcctgg
     3961 tgaagaccgt aagcgccggc aaagagcgcg ccggcaaaca cgacgccaac gatgagcaac
     4021 ttcgcgacag gcccgccaat ggccgtgccc gaaacggcga agccttggcg ccgcgcgaaa
     4081 tacgcaagca agctcaaatt tatccaggcg ccgatactgg tggcgagggc aaggccgact
     4141 tgcgccagcg ggcccatcag caccaccttc aagccaatgt tgacggtcgc ggcgagcagc
     4201 gccgctttca ccggcgtcag cgtatcgcgg cgcgcataga agggcgcggt gaaactccgc
     4261 agcagcacga agggaatgag tccaatcgag taagcggcaa gggtgttgcc cgcggcaagc
     4321 gcatcggccg ccgtgaacgc gccgcgcgca aacagcgccc gcatgatgag gtcgggaatg
     4381 gcgacggctg cggctgcgca cggcacggtc agaagcagcg ccagttggat gccgcgcgct
     4441 tgggccgacg ccgcgcccgc ctcgtccccc gccgccaagc gccgcgacat ctccggcaac
     4501 agcacgacgc cgacggcgat gcccacgacg ccgatgggca gctgattgat gcggtcggcg
     4561 tagtagagcg ctgacagcgc gccggtgggc aggaaggtcg cgatcagcgt atcggcgaag
     4621 agcgcgagct gcacaccgcc cgcgccgaga atggccgggc ctaaggcctt aaggaatttg
     4681 cgggtgggtt cgtcgagttt cggcatgcgc aagcgaatgc cgaaaccgtg ggcctcggcg
     4741 tcaaagccca acagaatggc ttgcgccaca ccggcaatga ggacacccca agccgccgca
     4801 tggccggcat cgggaaaata gggcgtcagc agcagtgtgg cgatcaccga gaggttcagc
     4861 agcacgggcg cgctggccgc cgcggcgaag cggttattgg cgttgagcac gccggcgaac
     4921 agggtctcca acgagacaag cgccagatag gggaaggtga tgcgcgtgag cgcgaccgcg
     4981 agatcgtagc gttccggatc gccgacaagg ccgggcgcga gaagcccgac gacttccggt
     5041 gtgaacagaa gcgcaatggc gagcagcacc agattgatgg tgaccagcgc cgcggcgatc
     5101 tgatcggcga agagcttggc cgccggcagg cccgcctcct ccagcgtgcg cgcataggcc
     5161 ggcacgaagg cggcggcgaa ggccccttcg gcgaagatgg cgcggaaatg gttcggcagg
     5221 cggaaggcga cgaagaaagc gtcggcgatc ggtccggcgc cgagaatggc agccatgacc
     5281 acatcgcgga tgaagccagt gaggcgtgag acgagcgtga gcccgccgac agtggcgatg
     5341 cgcctgatca tgcgcttggc tgatcctccc ttgaaggtcg ccctgctgaa ggtggacttg
     5401 gcttaatagt ccaccttgac caggtaaagg ccttggggcg gcgccaccgg cccgcaagcc
     5461 gcgcgatcct tggtttccaa ggcctttttg aggtcgccgc tcgtccagcg cccctcgccc
     5521 acgagcttca gcgaccccac catggagcgc acctgattgt gcaggaagga gcgggcagac
     5581 gcctcgatcc ggacgatctc gccgtggcgg gagacctcca gccggtccag ggtcttttcc
     5641 ggcgaagccg cttggcactc ggtggaacgg aatgtcgtga agtcgtgatg gccgatgagg
     5701 ccttgcgcgg cgtcgtgcat ggccttggcg ttcagaggct taaacacgcc ccaggcacgg
     5761 ccccgctcca gcgccagcgg gctgcgccgg tcgacgacgc gataaagata gtgccgtgcc
     5821 tttgcggaga agcgcgcgtc gaaaccgtcc ggcacctgct cgcaatccag caccgagatc
     5881 ggctcgggcc gcaattgcgc attgattgcg tcgcggatct tggccggcgc ccagtcccgt
     5941 tcgagatcga catgggcgac ttggccgatg gcgtggacac cggcgtcggt gcgcccggca
     6001 ccgcccggca tcgcatcctc gccggagaaa tccttgatcg cttgcgcgag ccgcccttgc
     6061 accgacatct gcccgtcctg acgctgccag ccgacaaagg gtgtgccgtc atattccagg
     6121 gtgagcttgt agcggggcat ggctaatcga aaaccgtgcg cgcgggaagg tctataccgc
     6181 gcaggaagga atcggccgac atcggctttt tgccggcgcg ctgaacctcg gtgagacgca
     6241 ccgcgccttc cccgcacgca accgtcagcg catcatcgag tagcatacct gcaggcgcat
     6301 tgtcgtcggc gagcttggag tcgagcacct tcacacgctc gcgcttgccg ccaaggttga
     6361 tctcgaacca agcgctcggg tacgggctaa gcccgcgaat atggttgtgc acgaccttgg
     6421 ctgccaacga ccagtcgatc cgcgtatccg cgttcttgat tttggccgcg taggtggcgt
     6481 tcgcatcgtc ttgcgcaaga cattgcagcg tcccgctctc aagtgccgcg agggcctcca
     6541 cgatcatgct tgcaccaagt tccgagagcg cgtcgtgcag ctgccccgcc gtcatgtccg
     6601 gcccaatgga ctcgcgctcc atgagacaga ccggcccttc gtcgagccct tcggtgatgc
     6661 gcatgatgca cacgccggat tcggcatcgc ccgccatgat cgcgcggttg atgggcgcgg
     6721 cgccccgcca gcgcggcagg agcgaggcgt ggatgttgaa cagcccgcgc cgcgtggcat
     6781 tgaggacttg cttgggcagg atgagcccat aggcgacgac cacggccgcc tccgcctcaa
     6841 gcgcggcgaa gcgcttctgc tcggctgggc tcttcaggtc gctcggcacg cgcacttcga
     6901 gaccggccgc ctccgcggcc tcgtgcaccg gcgatttccg caaggccatg ccgcgccccg
     6961 ccgcgcgggg cggctgggta tagaccgcgg cgacctcgtg gcccgccttg agaagcgcct
     7021 ggagcgccgg cacggcgaag tccggcgtgc ccatgaagat gatgcgcatg ggtccgggtc
     7081 ttaggctggt tgggaagcga agtgaagcct ttggcggcta catcgcctcg gcgcgcttct
     7141 gcttggtgaa tttcttgacg atcatgtcgc gcttgagtcg ggacaggtag tcgatgaaca
     7201 gcacgccgtt cagatgatcg acctcgtgct ggacgatggt ggagaggata ccttcgagca
     7261 cgcgctcttg gcgtttgccc tcgcggtcga tataggcgac gcggctcacg gcgggacgct
     7321 caacctcggc ggttacctcg gggatcgaca gacagccctc ctcgtggacg cgcaattcct
     7381 cggagcggtc gaggatcttc ggattgacca tgacgatggg gtccttaggc tcatcgtcgc
     7441 ccgtcgcgtc catgacgatc agc
//
LOCUS       DBNHVU010000077         5061 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1739302, whole genome shotgun sequence.
ACCESSION   DBNHVU010000077 DBNHVU010000000
VERSION     DBNHVU010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1739302"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="AC16Y7_02925"
     CDS             <1..605
                     /locus_tag="AC16Y7_02925"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIR5708364.1"
                     /translation="HLKAPAAASGMRIGLLGGSFDPAHEAHREISLAALKRLGLDQVW
                     WLVTPGNPLKDVSQLPDLKTRVAAAARVANHPRIVVTGYDGCTGSGYTIDLLKALRRR
                     FSDVYFVWLMGADNLVDFHRWKSWPEIFSMLPIAVLDRPGYRLKAKASQAAQRFAPYY
                     VDEIDAGGLALLEPPAWTILSHRLSGLSSTAIRENVLESG"
     gene            778..1188
                     /gene="rsfS"
                     /locus_tag="AC16Y7_02930"
     CDS             778..1188
                     /gene="rsfS"
                     /locus_tag="AC16Y7_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR5708365.1"
                     /translation="MRSPREGATHGTPPAGLASFRADPDRLLKVVLDTLDGSKAEDIV
                     VVDLKGKTSIGDHMVIASGRSQRHVGALADHLIKKLKDEGYGRARVEGQPQGDWVLID
                     AGDVIVHLFRPEVRDFYNLEKMWSADRPMERMVV"
     gene            1228..1710
                     /gene="rlmH"
                     /locus_tag="AC16Y7_02935"
     CDS             1228..1710
                     /gene="rlmH"
                     /locus_tag="AC16Y7_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR5708366.1"
                     /translation="MRLLIAAVGKLKQGPERELSTHYLGRAEALGRSLSLSPLSSIEL
                     AESRAANAALRRDAEAQALLAKVPSGSKRVCLDPGGKTLSSEDFAQVLGRLRDGGASG
                     AAFLIGGPDGLGRAALDGAELSLSLGPMILPHGLARIVLAEQLYRAMTILAGHPYHRG
                     "
     gene            1794..3158
                     /locus_tag="AC16Y7_02940"
     CDS             1794..3158
                     /locus_tag="AC16Y7_02940"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR5708367.1"
                     /translation="MAHSDLLAFTRQKTRVVLLLAGLLCVLPWTSYAQEDLSRDEAKK
                     RLDETEQELQSSRVKAKGLTKDLTALAEERARLNSALIEAGTRVQASEAKLSETEGKL
                     TELTEQVTVMRTSIDERKVAIVKMLSAMQRIGRTPPPAIVTRRDNALAVVRGAMLLAE
                     VFPELKYQADNLSHELEGLVRLENGIRTQRDEQKREAGRLAAERKRLDALLEERKSKQ
                     AQGEIELTRLEQSAEQLATEVTTLNELIENLDVRIAKAEVAQYDAEIAAEKALRERLQ
                     QEVMATPANENVIEIKPESTKVAFASPDRLKPAMPFERAKGTLRLPAKGRQTHQFGDP
                     DVAGAALQGLSLQTRKEARIIAPSDGWVVYAGPFRSYGQLLIINAGGGYHILLAGMSR
                     IDVSLGQFVLAGEPIAVMGNSAEPGSSGDTSSRPVLYVEFRKDGRPIDPGPWWVGESG
                     KVQG"
     gene            3155..4549
                     /locus_tag="AC16Y7_02945"
     CDS             3155..4549
                     /locus_tag="AC16Y7_02945"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR5708368.1"
                     /translation="MMRKTEYALLALLVLAVLAAGSTMVSFARSQAASATNSEIYKQL
                     DLFGEVLERVRADYVESPEDAKLIESAINGMLTALDPHSAYLNPKHFRDMQVQTRGEF
                     GGLGIEVTMENGVVKVVAPIEDTPAARAGLLSGDLVTHLDKEQIHGLTLQQAVEKMRG
                     PVNSPITLTVVRKGVEDPFEVKVVRDIIHINPVKYNVEGDDVGYVRLTTFNEQTTENL
                     QKAVADLKKKLGPKLKGYIVDLRNNPGGLLDQAISVSDSFLDQGAIVLTRGRNLEETQ
                     RSNARQGDITGGEKIVVLINGGSASASEIVAGALQDHHRATVIGTRSFGKGSVQTIIP
                     LGSNGALRLTTARYYTPSGRSIQAKGIDPEIVVEEELPEDIKKKEKVQGTRGESNLRG
                     HLKGEVGEDKEEEQSGSSSYVPEDKEKDTQLQYALDILRGIKSVDTEVKKQKAEANQK
                     AEAEQKAEAKAKAN"
     gene            4628..>5061
                     /locus_tag="AC16Y7_02950"
     CDS             4628..>5061
                     /locus_tag="AC16Y7_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708369.1"
                     /translation="MKALAVASGIVFWTLVLAAIVLIFFPSGNEGEPVAVLKVERSPG
                     GVAPIAPVTTDGVDLPPGFSVTGPAAVAPTTQRPAAPTLAPPGQGTTMVPTAPAPPPP
                     GQGTTMMPPPPPPPPGPSGALETPERQGALPSAEGAAEVVTA"
BASE COUNT         1002 a         1537 c         1651 g          871 t
ORIGIN      
        1 cgcatctcaa ggcgcccgcc gctgcatccg gcatgcggat cggtctcctc ggcggctcat
       61 tcgatccggc acatgaggcg caccgggaga tcagtctcgc ggcgttgaag cgcctcgggc
      121 tcgatcaggt gtggtggctg gtgacgccgg gaaatcccct gaaggacgtt tcccaactgc
      181 cggatctcaa gacgcgtgtg gcggcggcgg cgcgtgtggc gaaccatccg cggatcgtgg
      241 tgaccggcta tgacggctgc accggcagcg gttacacgat cgacctcctc aaggccctgc
      301 ggcggcgctt ttccgacgtc tatttcgtgt ggctgatggg tgcggacaat ctggtggatt
      361 tccatcgctg gaagtcttgg cccgaaatct tctcgatgct gccgatcgcg gtgctggacc
      421 gccccggcta ccggctgaag gcaaaagcga gccaggcggc ccagcgcttt gcgccctact
      481 atgtggacga gatcgacgca ggcggcctgg cgctcctcga gccgcctgcc tggacaatcc
      541 tcagccacag actgtccggc ctctcgtcga cggccattcg cgaaaatgtc ttggaatcag
      601 ggtagatcgc tagataagag caagaaaaac cccggcaata ctagctattg aaaggcccca
      661 acccggcata ctatgtttta ggggtgaagc ggcaatctag gggctcctca agcattgtgc
      721 gcgagacacg gccaaggccg cttccaggag caatggtaga aggataccca cgcaaacatg
      781 cgctcaccta gagagggtgc cacgcatggc acgcctccag ccggacttgc gtcattccgc
      841 gccgatccgg atagactgct caaggtcgtg ctcgacacgc tcgacggctc gaaggccgaa
      901 gacattgtcg tggtcgacct gaaaggaaag acctcaatcg gcgatcacat ggtgattgcc
      961 tcgggtcgtt cgcagcggca tgtcggtgct ctcgccgacc acctcatcaa aaagctgaag
     1021 gatgaaggtt atggccgcgc ccgcgtcgaa gggcagcccc aaggcgactg ggtgctgatc
     1081 gatgctggcg atgtcatcgt ccatctcttc cggcccgagg ttcgagattt ctacaatctc
     1141 gaaaagatgt ggtccgctga ccggcccatg gagcggatgg tggtctgagg aaagaccgcc
     1201 agcggcggca tgttcgcgag gtgagccatg cggctcttga tcgccgccgt cggaaagcta
     1261 aagcagggac ctgagcgcga gctcagcacc cattatctcg gccgcgccga ggcgcttggc
     1321 cggagcctat ctttgtcacc cctctcatcg atcgaactgg ccgagtccag agctgcgaac
     1381 gcggccctac gccgggacgc ggaggcgcaa gcgcttctcg ccaaggtgcc ttcgggctcc
     1441 aagcgcgtct gtctggatcc cggcggcaag acgctgtcga gcgaggattt cgctcaggtg
     1501 cttggcaggc tcagggacgg aggggcctcc ggtgcggcct ttctcatcgg cggcccggac
     1561 ggcctggggc gggcggcgct ggatggggcg gagctgtccc tctccctagg tccgatgatc
     1621 ttgccgcacg ggcttgcccg gatcgtgttg gcggagcagc tctatcgggc catgacgatt
     1681 ttggccgggc acccctacca ccgcggttga tctcctaagg ttaaggattc gtaagcgttt
     1741 tgccttgatt ctgccgcttc cctccggtgc catcgacgga acggccaggg cgtatggcgc
     1801 attccgatct attggctttc accagacaaa aaactcgggt cgtcctgctc ctcgccgggc
     1861 tgctttgcgt cttgccgtgg acctcttacg cgcaagaaga tctttcgcgc gacgaggcga
     1921 agaagcgtct cgacgagacc gagcaggaac tgcagtcgag ccgcgtcaag gcgaaggggc
     1981 tcaccaagga cctcaccgcc ctcgcagaag aacgcgcgcg gctcaatagt gcgctgatcg
     2041 aggccggtac gcgtgtgcag gccagcgaag cgaagctgtc cgagaccgag ggcaagctga
     2101 ccgagctgac cgaacaggtc acggtcatgc ggacctcaat cgacgaacga aaggtcgcca
     2161 tcgttaagat gctgagcgcg atgcaaagga ttgggcgaac gccgccgccg gccatcgtca
     2221 cgcgccgcga caatgcgctg gccgtggtgc gtggcgccat gttgctcgcc gaggtgttcc
     2281 ccgagctgaa atatcaggcg gacaatctct cccacgaact ggagggattg gtcaggctgg
     2341 agaacggcat ccgcacgcag cgcgacgagc agaagcggga ggccggccgg ctcgccgctg
     2401 agcgtaagcg gctcgacgcg ttgctcgagg agaggaagtc gaagcaggcg caaggcgaga
     2461 tcgagctgac ccggctggag cagtccgccg agcagctggc gaccgaggtc acgaccctga
     2521 acgagctcat cgagaatctc gacgttcgaa ttgccaaggc cgaagtggcg caatacgacg
     2581 ccgagatcgc ggctgagaag gccttgcgcg agcggttgca gcaggaagtc atggcaactc
     2641 cggcgaatga gaatgtgatc gagatcaagc ccgaatcgac caaagtggcc tttgccagtc
     2701 ccgatcggct gaagccggcc atgccgttcg agcgggccaa aggcacgcta aggctgcccg
     2761 ccaaggggcg ccagacgcat cagtttggcg acccggatgt cgcaggcgcg gccctccagg
     2821 gcctatctct ccagacccgc aaggaagcgc ggattatcgc gccctcggac ggttgggtgg
     2881 tctatgcggg gcccttccgc tcctatggcc aactcttgat catcaatgcg ggcgggggct
     2941 atcatatttt gcttgcgggg atgagccgca tagatgtgag ccttggccaa ttcgttctag
     3001 ccggcgagcc catcgctgta atgggcaatt cagcggagcc aggcagcagc ggcgatacta
     3061 gttcacgccc cgtcctctat gtcgaattca ggaaggacgg tcggccaata gaccccggcc
     3121 catggtgggt tggggaatcc gggaaggtgc aaggatgatg cggaaaaccg aatacgcgct
     3181 tttggcgctt ctggtgctgg ctgtgctcgc cgctgggtcg accatggtga gctttgcccg
     3241 aagccaggcg gcatccgcca ccaactccga gatctacaag cagctcgacc tttttggcga
     3301 ggttctggag cgcgtgcgcg ccgactatgt cgagtcgcct gaagacgcca agctcatcga
     3361 gtccgcgatc aacggaatgc tgaccgcgct cgatccgcac tccgcctatc tcaatccgaa
     3421 gcacttccgc gacatgcagg tacagacccg cggcgagttc ggcgggctcg ggatcgaagt
     3481 cacaatggag aatggtgtcg tgaaggtcgt ggcgccgatc gaagatacgc cggctgcgcg
     3541 tgccggcttg ctgagcggcg atctcgttac gcatctcgac aaggagcaga tccacggtct
     3601 gacgctgcaa caggcggtgg agaagatgcg cgggccggtc aactcgccga tcacgctgac
     3661 ggtcgttcgc aagggcgtcg aggacccctt cgaggtgaag gtcgtccgcg acatcattca
     3721 tatcaacccg gtgaagtaca acgtcgaggg cgacgatgtg ggctatgtcc ggctcaccac
     3781 cttcaacgag cagaccaccg agaacttgca gaaggccgtt gcggacctga agaagaagct
     3841 cggtcccaag ctcaagggct acattgtcga cctgcgcaac aatccgggcg gcttgctcga
     3901 ccaggctatc tcggtctcgg attcgtttct cgaccaaggg gccatcgtgc ttacgcgtgg
     3961 gcgcaatctc gaagagacgc agcgctcgaa cgcgcggcaa ggcgatatca cgggcggtga
     4021 gaagatcgtc gtgttgatca atggcggctc cgcgtccgca tcggagatcg tcgccggcgc
     4081 gctgcaggac catcaccgcg ccaccgtcat cgggacgcgc tcgttcggta agggctcggt
     4141 gcagacaatt atcccgcttg gctccaacgg cgccttgcgg ctgaccaccg ctcgctacta
     4201 cacgccgtca ggccgttcga tccaggccaa gggtatcgac ccggagatcg tggtcgaaga
     4261 ggagcttccc gaggacatca agaagaagga aaaggtccaa ggtacgcgcg gtgagtccaa
     4321 tctgcgtggc cacctgaagg gcgaggtcgg cgaagacaag gaagaggagc agtccggaag
     4381 ttcgtcatac gtgcccgagg acaaggagaa ggacacgcag ctacaatacg ccctagatat
     4441 cttgaggggc atcaagtcgg tcgacacgga agtcaaaaag cagaaggcgg aagccaacca
     4501 gaaggcagaa gccgagcaaa aggcggaagc caaggcgaaa gccaattagt ctttcggtga
     4561 tcgaattgcc ccggagcgtt ggcgtaagcc ccataagacg gccgggcatt gggggaggac
     4621 gacgacgatg aaagccctgg ccgttgcttc ggggattgtg ttttggacgt tggtgcttgc
     4681 cgccatcgtt ctcatcttct tccctagcgg caatgaaggc gagcccgtcg ccgtcctgaa
     4741 ggtcgagagg tctccgggcg gggtggcgcc tatcgcgccc gtgacgaccg acggcgtcga
     4801 tcttccgcca ggcttctccg tgacaggccc cgccgccgtt gccccgacga cccagcgccc
     4861 agcagcgccg acgctcgcgc cgcccggcca gggcaccacc atggtgccga cggcgccggc
     4921 accgccgccg cctggtcagg gcaccaccat gatgccgcca ccgccaccgc cgccaccagg
     4981 tcccagcggg gcgcttgaga cgcctgagcg gcagggcgca ctgccttcgg cagagggtgc
     5041 ggcggaagtc gtgacggcca a
//
LOCUS       DBNHVU010000078         5737 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1143646, whole genome shotgun sequence.
ACCESSION   DBNHVU010000078 DBNHVU010000000
VERSION     DBNHVU010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5737)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5737)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5737
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1143646"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="AC16Y7_02955"
     CDS             complement(<1..287)
                     /locus_tag="AC16Y7_02955"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HIR5708370.1"
                     /translation="MTPAFGTLRICENAGDLAQKGATDLCDRAAANPGRTIIALSGGN
                     TPKPLYALLAQEPILSQMPWDRVHFILGDERFVPPSDPASNYGMVRAAFLD"
     gene            440..1111
                     /gene="rpe"
                     /locus_tag="AC16Y7_02960"
     CDS             440..1111
                     /gene="rpe"
                     /locus_tag="AC16Y7_02960"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR5708371.1"
                     /translation="MTHEIIIAPSILSADFAKLGEEVRAVDKAGADWIHIDVMDGHFV
                     PNITIGADVVKAIRPHTKKPFDVHLMIAPCDPYIENFAEAGADMVTVHVGAGPHLDRS
                     LQLIRSFGMRAGVSLTPSTPEGTIEYVLDKVDHVLVMTVNPGFGGQTFIPAQLNKIRA
                     IRKMIGGRPIHLEVDGGVHLETAPLVVEAGANALVSGSGVFDGGSYVENIKALREAAM
                     RGRGV"
     gene            complement(1113..2741)
                     /gene="pgi"
                     /locus_tag="AC16Y7_02965"
     CDS             complement(1113..2741)
                     /gene="pgi"
                     /locus_tag="AC16Y7_02965"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIR5708372.1"
                     /translation="MSTQLTDTPAWQALSAHHAKIKDAHLRDLFAGDAKRAGRFTAEG
                     AGLTLDYSKNRITEETISLLASLAKERKVAERRDAMFRGEKINVSENRAVLHVALRAP
                     RGEVIKVDGHDVVPDVHTVLDRMAAFADKIRSGEWKGHTGKRIKNVINIGIGGSYLGP
                     EMAYLALRPYSDHDMTFRFVSNVDGACFDEATRDLNAEETLFIVSSKTFTTLETMTNA
                     ATARAWAVEKLGSEDVVAKHFVANSTNTEGVEKFGIDTANMFEFWDWVGGRYSMDSAI
                     GLSTMIAIGPENFRDMLAGFHEMDEHFRTAPIEKNLPMLMGLLTVWYNNFFGAQTLGV
                     MPYSEHMARFPAYLQQLQMESNGKHVDLAAAHVNYQTGPIIWGEPGTDGQHSFYQLIH
                     QGTKLIPCDFIGFLDPLSSLAHHHDLLMANLFAQAEALAFGKTAAELTAEGSHAKQTP
                     FRVTEGNLPTNMILAEKLDPKTLGRLVALYEHSVFTQGAVWDIDSFDQWGVELGKVLA
                     VRIIPELEAKAAPKLSHDSSTNALIERYRKHKFS"
     gene            complement(2754..3830)
                     /gene="tal"
                     /locus_tag="AC16Y7_02970"
     CDS             complement(2754..3830)
                     /gene="tal"
                     /locus_tag="AC16Y7_02970"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HIR5708373.1"
                     /translation="MKPTQALHDLGQSLWVDNITRDMLDSGTLKKYIDEFSATGLTSN
                     PTIFDNAISKSDSYDPEIERLKKAGYSGEALFFELALQDLSGAADLFLPIHWTTATVD
                     GMVSLEVSPLLAYDTEATIAEAKRLFERAHKPNLFIKIPGTKEGLPAIEEAIFSGVNV
                     NVTLLFSSEHCKAAADAYMRGLERRVRAGLDPDVRSVASIFISRWDKAVMDKVSDDLR
                     DKLGIAVAHEAYRDYRAGLHTDRWQRLESFGARPQRLLFASTSTKDPRASDTLYIGGL
                     AAPNTVNTMPDKTMEAFADHGKLNGALSPDGGNCKEVFAAYEKVGISVSGLAAQLQKD
                     GADSFVKSWQNLLQSIETKSKALG"
     gene            complement(3846..4520)
                     /locus_tag="AC16Y7_02975"
     CDS             complement(3846..4520)
                     /locus_tag="AC16Y7_02975"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR5708374.1"
                     /translation="MSETRDLVFLFDVDNTLLDNDRMKHDLGDYVSQTFGPEARDELW
                     KLYEEERIKHGYADFLGTLERFRLAHLHDPHILQLANWVLDYPFIERLYPDALAVVRH
                     VRQWGLPVILTDGDGVFQPHKLERAGLLGAFSGHVLNYVHKEQELDSVTRAYPAKHYV
                     LIDDKLSLLDAVKKAWGTRVTTVFPRQGHYANDAKVLARSQPADIEIERIADLMEHDC
                     SALQPA"
     gene            complement(4531..>5737)
                     /gene="zwf"
                     /locus_tag="AC16Y7_02980"
     CDS             complement(4531..>5737)
                     /gene="zwf"
                     /locus_tag="AC16Y7_02980"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIR5708375.1"
                     /translation="DSVKAHGGDIKSEAFKKLLSLLRYVDGDYNDPKVFKQLRKELGD
                     AKAPLHYLAIPPALFSVVASQLAKSGCADNARLVVEKPFGRNRRSANDLSRILDQYFS
                     ERNIFRIDHYLGKEPVQNILYTRFANSIYEPVWNRHFVRSIQITMAENFGVQDRGAFY
                     DSAGAIRDVLQNHMLQILASLTMDPPTGQAHEAIRDRKASLLKAVRTLDSDDIVRGQY
                     TGYEKVPGVKPGSTVETFAAAKLCIDSWRWEGVPIFIRTGKCLPVSIAEAVVQFKKPP
                     RETFGEIDSLGCGHMRFRIQPDVTIAFGTRVKVPGERMIGEDVELILTRQPGVDKPPY
                     QRLLGDAMEGISDLFTREDIVDAEWRIVNDVLGDVTPVYHYEPGTWGPSEAKQLIGRD
                     GPWLDPKPV"
BASE COUNT         1044 a         1721 c         1815 g         1157 t
ORIGIN      
        1 tggtcgagaa acgccgcccg caccatgccg taattgctgg cggggtcgga tggcggcacg
       61 aagcgctcat cccccagaat gaaatggacg cggtcccagg gcatctgcga gaggattggc
      121 tcttgggcca gaagcgcgta gagcggcttc ggcgtattgc cgccggaaag cgcgatgatc
      181 gtgcgccccg gattggccgc tgcgcggtcg cacagatcgg tggcgccttt ttgcgccaga
      241 tcgcccgcgt tctcgcagat tcgaagcgtc ccaaaagccg gtgtcatacg tggtgcctcg
      301 ctcgcctcac caaattgcgc cggcacgata ggggcagaaa gttaagcgtg ccgcaaggtg
      361 agaaatgtaa agtgctgatg gtgcccctga gtcgggctag gctgcggtca tatatgagag
      421 cgccatgtat gagagcgcca tgacacacga gatcatcatc gcgccgtcga tcctctccgc
      481 cgacttcgcc aagctcggcg aggaagtgcg tgccgtggac aaggcaggcg ccgactggat
      541 ccatatcgac gtcatggacg gccacttcgt gcccaacatc accatcggcg cggacgtggt
      601 aaaggcgatc cggccgcaca cgaaaaagcc cttcgacgta catctgatga ttgcgccgtg
      661 cgatccgtat atcgagaatt ttgccgaggc cggtgcggac atggtcacgg tgcatgtggg
      721 agcggggccg cacctggacc gctcgttaca gctcatccgt agcttcggta tgcgggctgg
      781 agtcagcctc accccgtcta ctcctgaggg cacgattgaa tatgtgctcg acaaggtgga
      841 ccatgtcctg gtgatgacgg tgaaccccgg cttcggcggc cagaccttca tcccggcgca
      901 gctcaacaaa atccgcgcca tcagaaagat gatcggcgga cgtcccatcc atctcgaagt
      961 cgatggcggc gtgcatctgg aaaccgcgcc tctcgtcgtc gaggccggcg ccaatgcact
     1021 tgtctccggc tctggcgtgt tcgacggcgg gtcttacgtt gagaacatca aggcgcttcg
     1081 agaggccgcg atgcgtggcc gcggcgtcta gcctacgaga acttgtgttt gcggtaacgc
     1141 tcgatcaggg cattggtcga actgtcatgt gacagcttcg gcgcggcctt cgcttccagc
     1201 tccgggatga tgcgtacagc gagcaccttg ccgagctcca cgccccactg atcgaacgag
     1261 tcgatgtccc acacggcgcc ttgggtgaac acgctgtgct cgtaaagcgc aaccaatcgc
     1321 cccaaagttt tgggatcgag cttttcggcg aggatcatgt tggtcggcag gttgccttcg
     1381 gtgacgcgga acggcgtctg cttcgcatgc gatccttccg ccgtcagttc cgctgccgtc
     1441 ttgccgaacg ccaaagcttc agcctgcgcg aacaaattcg ccatcagcag atcgtggtgg
     1501 tgggcaagcg agctcaacgg gtcaagaaag ccaatgaagt cgcacgggat cagcttcgtg
     1561 ccctggtgga tgagctgata gaacgagtgc tggccgtccg tgccaggctc gccccagatg
     1621 atcggcccgg tctggtaatt cacatgcgcc gcggcaagat cgacatgctt gccgttgctc
     1681 tccatttgca gctgctggag ataggcgggg aagcgcgcca tgtgctcgga gtacggcatg
     1741 acgccgaggg tctgcgcgcc gaagaaattg ttgtaccaga cagtgagcag gcccatcagc
     1801 atgggcaggt tcttctcgat cggcgccgtc ctgaagtgct cgtccatctc gtggaagccc
     1861 gcgagcatgt cgcggaagtt ctccggcccg atggcaatca tggtcgacag gccaatggcg
     1921 gagtccatgg aatagcggcc gccgacccag tcccagaact cgaacatgtt ggcggtgtcg
     1981 atgccgaatt tctcgacgcc ttccgtattg gtggagttgg cgacgaagtg cttggcgacg
     2041 acgtcctccg agccgagctt ctccaccgcc caggcccgcg ccgtggcggc gttggtcatg
     2101 gtctccagcg tggtgaaggt cttcgacgaa acgatgaaca gcgtctcttc ggcattgagg
     2161 tcgcgcgttg cttcgtcgaa acaggcgccg tctacgttgg agacgaagcg gaaggtcatg
     2221 tcgtggtccg aataggggcg gagcgccaga tacgccatct ccggtccgag ataggagccg
     2281 ccaataccga tattgatgac gttcttgatg cgttttcccg tgtgaccttt ccattcgccg
     2341 ctacggatct tatcggcgaa cgccgccatg cggtcgagca cggtgtgcac gtccggcacc
     2401 acgtcgtgcc cgtccacctt gattacctcg ccgcggggcg cgcgcaaagc aacgtgcagc
     2461 acggcgcggt tctccgagac attgatcttc tcgccacgga acatggcatc gcgccgctcg
     2521 gcgaccttcc gctccttggc gaggctcgcc agaagactga tcgtctcttc ggtaatgcgg
     2581 ttcttcgagt aatcgagggt cagcccggcg ccttcggctg tgaacctacc tgcgcgtttc
     2641 gcatcgccgg caaacagatc gcgcagatgc gcatccttga tcttggcgtg atgggcggac
     2701 agcgcctgcc acgctggcgt gtcggtcaat tgcgtggaca ttggcaagcc tccctagcct
     2761 agcgccttgg attttgtttc gatggattgg agcaggttct gccacgattt cacgaaggag
     2821 tcggcgccgt ctttttgaag ttgcgccgcc agtcccgaca ccgaaatgcc caccttctca
     2881 taggccgcga acacctcttt gcaattgccg ccgtccggcg acagcgcgcc gttcagtttg
     2941 ccgtgatcgg cgaaggcctc catcgtcttg tcgggcatgg tgttgaccgt attcggtgcg
     3001 gccagcccac cgatatagag cgtgtcggac gcgcgcggat ccttggtgct ggtggacgcg
     3061 aacagcaggc gctgcggtcg cgccccgaac gactccagcc gctgccagcg atcggtgtgg
     3121 aggccggcgc ggtagtctct gtatgcctcg tgcgcgacgg cgatgccgag cttgtcgcgc
     3181 aaatcgtcgg agaccttatc catgaccgcc ttgtcccagc ggctgatgaa gatcgaggcc
     3241 acggagcgca cgtccgggtc gaggcctgcg cgcacgcggc gctccaggcc ccgcatataa
     3301 gcgtccgccg ccgccttgca gtgctcggag gagaacagca gcgtgacgtt gacgttcacc
     3361 cccgagaaga tcgcctcttc gatggcgggc agcccttcct tggtgccggg gatcttgatg
     3421 aacaggttcg gcttatgcgc ccgttcgaag agacgcttcg cctcggcgat ggtggcctcg
     3481 gtgtcatagg cgaggagagg ggagacttcg agcgagacca tcccgtcgac agtggcggtc
     3541 gtccaatgga tcggcaggaa caaatccgcc gctcccgaca gatcctgcaa cgccagctcg
     3601 aagaacagcg cttcgccgga atagccagcc ttcttcaggc gttcgatctc cggatcgtag
     3661 ctatcgctct tggagatggc gttgtcgaag atcgttgggt tggaggtcag cccggttgcg
     3721 gagaactcgt cgatgtattt cttcagcgtt ccgctgtcga gcatgtcgcg ggtgatgttg
     3781 tcgacccata ggctctggcc caggtcgtgc aaggcttgcg tgggtttcac gggttcgtct
     3841 cctcctcagg ccggctgaag cgccgaacaa tcgtgttcca tcaaatcggc gatgcgttcg
     3901 atctcgatat ccgctggttg tgaccgcgcc aagaccttcg catcgttggc atagtgtccc
     3961 tggcgcggaa agaccgtagt gacgcgcgtc ccccacgctt tcttcaccgc atccaaaagg
     4021 ctgagcttgt cgtcgatcag cacgtaatgc ttggccggat aggcgcgggt aacggagtcc
     4081 agctcctgct ccttgtggac atagttgagc acatggccgc tgaacgcccc caaaaggccg
     4141 gcccgctcca gcttgtgcgg ctggaatacg ccgtcgccgt cggtaaggat taccggcaag
     4201 ccccattggc ggacgtggcg caccacggcg agcgcgtccg gataaaggcg ctcgataaac
     4261 ggatagtcca agacccaatt tgccagctgc aaaatgtgcg gatcgtgcag atgcgccaag
     4321 cggaagcgtt ccagcgtgcc gaggaaatcc gcatagccgt gcttgatgcg ctcttcttcg
     4381 tatagcttcc agagttcgtc ccgcgcttcg gggccgaagg tctggctcac atagtcgccg
     4441 agatcgtgct tcatgcggtc gttgtcgagc aacgtgttgt ccacgtcgaa caaaaagaca
     4501 aggtcgcgcg tctcggacat cgggccccgt ctacacaggt ttggggtcga gccaaggacc
     4561 gtcgcggcca atcagctgct tggcctcgct gggcccccaa gtaccgggct cgtagtggta
     4621 gaccggggtc acgtcgccga gcacatcgtt cacgatgcgc cattccgcat cgacgatgtc
     4681 ctcccgcgtg aacaggtcgc tgatgccttc catggcgtcg cccaagaggc gctgataagg
     4741 cggtttgtcc acgcccggtt gccgcgtcag gatcagctcg acgtcctcgc cgatcatgcg
     4801 ttcgcccgga accttgacgc gagtgccgaa ggcgatggtc acgtcgggct ggatgcgaaa
     4861 gcgcatatgc ccgcagccga gcgagtcgat ctcgccgaag gtctcgcgcg gcggcttttt
     4921 gaactgcacc acggcctcgg cgatgctgac aggcagacac ttgccggtcc ggatgaagat
     4981 aggcacgcct tcccagcgcc agctgtcgat gcacagcttc gcggcggcga aggtttcgac
     5041 cgtcgagccg ggcttcacgc ccggcacttt ctcgtagccg gtatactggc cgcgcacgat
     5101 gtcgtcggag tccaaagtcc gcacggcctt cagcaggctt gctttgcgat cgcggatcgc
     5161 ttcgtgggct tggcccgttg gcgggtccat ggtcagggat gcgagaatct gcagcatgtg
     5221 attttgcaaa acgtcacgga ttgcgccggc gctgtcgtag aacgcaccgc ggtcctgcac
     5281 gccgaaattc tcggccatgg tgatctggat gctgcgcacg aagtggcggt tccagaccgg
     5341 ctcgtaaatc gaattggcga agcgagtgta gagaatgttc tgcaccggtt ctttgcccag
     5401 atagtggtcg atgcggaaga tgttccgctc ggagaaatac tgatcgagga tgcggctgag
     5461 gtcgttggct gaccggcggt tgcgtccgaa cggcttctcg accacgaggc gcgcattatc
     5521 cgcgcagcct gacttggcaa gctgcgaggc gaccacggag aacagcgcgg gcggaatggc
     5581 gagatagtgc aggggagcct tggcgtcgcc cagctctttg cgcagctgct tgaacacctt
     5641 ggggtcgttg taatcgccat ccacgtagcg caacaggctc aagagctttt taaaggcctc
     5701 ggacttgatg tcgccgccat gggctttgac gctgtcc
//
LOCUS       DBNHVU010000079         7584 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_773278, whole genome shotgun sequence.
ACCESSION   DBNHVU010000079 DBNHVU010000000
VERSION     DBNHVU010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7584)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7584)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7584
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_773278"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="AC16Y7_02985"
     CDS             <1..258
                     /locus_tag="AC16Y7_02985"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5708376.1"
                     /translation="SPDVSDFPQESLHHLKGLDIWIIDALRYTHHPSHLSLEQALAWI
                     ERLAPKRAVLTHMHVDLDYETLKDQLPDGVEPAYDGMVLGS"
     gene            complement(297..1007)
                     /locus_tag="AC16Y7_02990"
     CDS             complement(297..1007)
                     /locus_tag="AC16Y7_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015952194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5708377.1"
                     /translation="MSEQRLKGRIALITGASRGIGRAAARLFAREGAHVLLLARQQKT
                     LETVDDEIKAEGGVASIIPLDLEDGQTIDALGPALYERFGRLDILVGNAGVLGGLRPL
                     THIPSEIWDRVLAVNLTANWRLIRTLDPLLQRSDAGRVVFVTSPRAASLGRAYWAPYS
                     VSKAALETLAKTYANETADSPICVNLVDPGATATAMRAEAFPGEDQDTLKTPEAVAEV
                     ILRLALPDFTETGEIVAV"
     gene            complement(1004..2491)
                     /gene="purF"
                     /locus_tag="AC16Y7_02995"
     CDS             complement(1004..2491)
                     /gene="purF"
                     /locus_tag="AC16Y7_02995"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR5708378.1"
                     /translation="MPVTEQAEVSDPVLNWLEEDRLHEECGVFGVFNHPDAAALTALG
                     LHALQHRGQEAAGIVSFDGEHFHTERRLGLVGDHFTSRPVIDRLMGDMAVGHVRYSTC
                     GDAVLRNVQPLFVDLESGGFTLCHNGNLTNALTLRESLIRKGAICQSTTDTEVILHLV
                     ARSEKTQFVERFIEALLQVEGAYAFVGMTNKKLVGARDPFGIRPLVLGRLGDAWVLSS
                     ETCALDIIGAEFVREVENGEVVVATREGGLESHRFVPKHPARLCIFEYIYFARPDSII
                     GGRSVYDVRKEMGLQLAREAPADADMVVPIPDSGVPAAIGYAQESGLPFEFGIIRNHY
                     VGRTFIEPEQRIRQLGVKLKHSANELQVKGKRIVLIDDSVVRGTTSVKIVQMMREAGA
                     KEVHMRVSSPPITHPDFYGIDTPDQDKLLAANRSLEEMRSFIGADSLAFISVDGIYRA
                     MGLEARDDDNPQFTDHCFTGEYPTRLRDREGPPVQKQLSFLAEVG"
     gene            complement(2566..3138)
                     /locus_tag="AC16Y7_03000"
     CDS             complement(2566..3138)
                     /locus_tag="AC16Y7_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR5708379.1"
                     /translation="MPISWLDIILIVIMLISGFLAMVRGFTREVLSIFSWAMAAVAAL
                     YFTPRYYEVLIPYIDNPSIAQIAFAAGVFIITLIIVSLITFRISDKILDSRVGALDRS
                     LGFIFGLARGFLLVAIVFILFTALARDQPEWVRNARSYPLLQRTQVAIESLLPMHPEE
                     LLPGDSDQPEGAPDGEPGTDAAPTPPEQPS"
     gene            complement(3262..4644)
                     /gene="radA"
                     /locus_tag="AC16Y7_03005"
     CDS             complement(3262..4644)
                     /gene="radA"
                     /locus_tag="AC16Y7_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR5708380.1"
                     /translation="MAKVSKAYVCQSCGAVTNKWTGKCPSCGAWNSIIEEASTPTSSP
                     ALVAIKGGKGRLASLETLSAETEGASRIKTDIAELDRVLGGGLVPGAAVLVGGDPGIG
                     KSTLLLQTAAELSQDGHRIVYLSGEEAPAQVRMRASRLGVAEAPVALGTETNIASIIA
                     TLGSGPAPALVVIDSVQTLWSEGIESAPGTISQLRGCASALISYAKASGTTLVLVGHV
                     TKDGQIAGPKVIEHMVDTVLYFESLSGHQFRILRAVKNRFGPTDEIGVFEMRSEGLKE
                     VENPSALFLNDADRGTPGTAVLAGMEGTRPILVEIQALVAPSSLGTPRRAVVGWDPNR
                     LSMLLAVLEARCGVRLGSHDVYLNVAGGLRLSEPAADLAAAAALLSSFTGRAVPEDGV
                     YFGEVSLTGAVRAVGHTDQRLKEAAKLGFARAVVPAASLSAEREVEGIALNPVSQLDE
                     LVAWFALIGD"
     gene            complement(4656..5807)
                     /gene="alr"
                     /locus_tag="AC16Y7_03010"
     CDS             complement(4656..5807)
                     /gene="alr"
                     /locus_tag="AC16Y7_03010"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR5708381.1"
                     /translation="MSHARTRDAAADNKVPPDSTALLTIDLNALIANYDRLRELAAPA
                     ECAAVVKADAYGLGVTEVAPALWRAGCKTFFVATLPEAEELRALLPEAVIYVFSGLLP
                     GTADIYRKADLRPVLNSAEEIREWAAQCSRYGEALPCAVHIDSGMNRLGLTAADVEAV
                     SNNRELWSALSLSLVMSHLACADDASHAKNAAQRDIFDSLRARLPETRASLANSAGIL
                     LGADFVYDLVRPGIALYGGHPQRRGPNPFQPVVHLIGRILQVREAEPGETVGYGATRT
                     LPNGARIAIVSVGYADGLFRALSVADGGEGLAVRIGDHAAPILGRVSMDLIAIDVTDV
                     PKTHARRGEWVELLGHNISAHDLASHANTIDYEVLTSLGRRAVRRFVGG"
     gene            complement(5804..7285)
                     /locus_tag="AC16Y7_03015"
     CDS             complement(5804..7285)
                     /locus_tag="AC16Y7_03015"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR5708382.1"
                     /translation="MTALIRPADTSETPVFRESPHNLEAEQALLGAILVNNEALDRVS
                     TFLKPGHFFDRLHGRIFESASKLIMGGKRATPITLKTFFQNDEPVADLPVPQYLGRLA
                     ANATTIINAEDYGRTVYDLAIRRQLIDIGEGMVHTAFDSPIDAAPSDQIEDAEQQLYE
                     LAETGKYGAGFEPFSAALTDAIDMAANAYQRDGGLSGIATGFSDLDQRMGGLQASDLI
                     IIAGRPALGKTALATNIAYHVAKANAANAGGDTPADGAVVGFFSLEMSAEQLATRIIS
                     EQSYIPSERIRRGRIDSDQFDRIVEVSQELQNLPLFIDQTGGITVAQLAARARRLKRQ
                     RGLGFIVVDYLQLLSGSSRRAQEGRVQEVSEITTGLKALAKELHVPILALSQLSRQVE
                     QREDKRPQLADLRESGSIEQDADVVMFVFREEYYLERSQPRENTEEHRQWQQDMETVT
                     GKADVIIGKQRHGPTGTVTLQFTPEFTRFSNLAPSERLPERSE"
     gene            complement(7381..>7584)
                     /locus_tag="AC16Y7_03020"
     CDS             complement(7381..>7584)
                     /locus_tag="AC16Y7_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR5708383.1"
                     /translation="RFCRMWEMYLAGSETGFRYQNLMVFQLQVTKDQCALPLTRDYFF
                     DAERELRMRERHASPQRKRRSSR"
BASE COUNT         1356 a         2287 c         2563 g         1378 t
ORIGIN      
        1 tcgcctgacg tcagcgattt cccgcaagag tcgctccatc atctcaaggg gctcgacata
       61 tggatcatcg acgcccttcg ctacacgcac catccgagcc atttatcttt agagcaggcg
      121 ctggcgtgga tcgaacggct cgcgccgaag cgcgccgtgc tgacccacat gcatgtggac
      181 ctcgactacg agacgctgaa ggatcaactg cctgacgggg tcgagcccgc ttatgacggt
      241 atggtgcttg gttcgtagtg tcgcgcccgc ctgcttagtc cgcgaaggcg tagcactcac
      301 acggcgacga tctcgcccgt ctctgtgaag tctggcaggg caaggcgcag gatgacttct
      361 gccaccgcct caggcgtctt cagcgtgtcc tggtcctcgc ccggaaacgc ctcggcacgc
      421 atggcggtgg ccgtcgcccc ggggtcaacg aggttgacgc agatcggact gtcggcggtt
      481 tcgttcgcgt aagtttttgc gagcgtttcg agtgccgcct tggagacgga gtagggcgcc
      541 cagtaggcgc ggccgaggct cgcggcgcgc ggcgaggtga cgaatacgac ccgccctgca
      601 tcggagcgtt ggagtaacgg gtccaaggtg cggatcaggc gccaattggc ggtgaggttg
      661 acggccagca cgcggtccca gatctcagac gggatatgag tcagcgggcg taagccaccg
      721 agaacgcccg catttccaac caggatgtcg agccggccga agcgctcata aagcgctggg
      781 cccagagcgt cgatggtctg tccgtcctcg agatcgaggg ggatgatcga ggcaacgccg
      841 ccctcggcct tgatctcgtc atccacggtc tcgagcgtct tttgctgccg cgccagcaac
      901 agaacgtgcg cgccttcgcg cgcgaaaagg cgcgcggctg cgcgtccgat gccacgcgag
      961 gcgccggtga tcagcgcgat acggccttta agacgttgct cgctcaaccg acctcggcga
     1021 ggaaggaaag ttgcttctgg acgggcgggc cttcgcggtc gcgtaggcgc gtcgggtatt
     1081 cgccggtgaa gcagtggtcg gtgaattgcg gattgtcgtc gtcgcgggcc tcaaggccca
     1141 tggcccgata gatgccgtcg acagaaatga aggccagtga atctgcaccg ataaaggagc
     1201 gcatctcctc aagcgagcgg tttgctgcga gtagtttgtc ttggtcgggc gtgtcgatgc
     1261 cgtagaagtc cggatgcgtg atcggcgggc tcgaaacgcg catatgcact tcctttgcgc
     1321 cggcctcacg catcatttgc acaatcttca ccgacgtggt tccgcgcacg acgctgtcgt
     1381 cgatgagcac gatacgtttg cccttcacct gaagctcatt ggcgctatgc ttcagcttga
     1441 cgccaagctg gcgaatgcgt tgctccggct caataaaggt gcggcccaca taatggttgc
     1501 ggatgatgcc gaactcgaac ggcaggccgg actcttgcgc ataaccgatg gccgcgggca
     1561 cgccgctatc cgggatcggt accaccatgt cggcgtcggc gggggcttcg cgtgccagct
     1621 gcaggcccat ttccttgcgc acgtcgtaga ccgaacggcc gccgatgatg gaatcggggc
     1681 gggcgaagta gatgtattcg aagatgcaca gccgtgcggg atgcttgggc acgaagcggt
     1741 ggctctccag cccgccttcg cgggtggcaa cgacgacctc gccattttcc acctcgcgga
     1801 cgaactctgc gccaatgatg tcgagcgcgc aggtctcgga ggaaagcacc caggcgtcgc
     1861 ccaagcggcc gagcacgagt ggccggatcc cgaatggatc gcgcgcgccc accagctttt
     1921 tgttggtcat gccgacaaaa gcgtaggcac cttcgacctg gagtagcgcc tcgatgaagc
     1981 gctccacgaa ttgggtcttc tcgctacgcg ccacgaggtg aaggatcacc tcagtgtcgg
     2041 tcgtcgactg gcagatggcg ccttttctga tgagggactc gcgcaaagtg agcgcattgg
     2101 tgaggttgcc gttgtggcag agcgtgaagc cgccggactc aaggtcgacg aagagcggct
     2161 ggacgttccg cagtacggca tcgccgcagg tcgagtatcg aacatggcca acggccatgt
     2221 cgcccatcag gcggtcgatc acggggcgtg aggtgaagtg gtctccgacg aggcccaggc
     2281 ggcgctcggt gtggaagtgc tcaccgtcga agctgacgat gccggcggct tcctggccgc
     2341 gatgctggag tgcgtgtagg ccgagcgcgg tcagcgcggc ggcgtcggga tggttaaaca
     2401 ctccgaacac gccgcactcc tcgtgcagcc tatcctcttc cagccagttc aagacagggt
     2461 cgctaacctc ggcctgttcg gtcacgggca tggcctttct tctccataaa taggcgccct
     2521 ggcgagggct tcaatgccac gcaacttgtt tcgttactga tgcgcttacg acggctgttc
     2581 tggcggcgta ggcgcggcgt ccgtccccgg ttcgccgtcg ggtgcgccct cgggctgatc
     2641 ggagtctcca ggaagcaact cttccggatg catggggagc agagactcga tcgccacctg
     2701 cgtcctttga agaaggggat aagagcgcgc gttgcggacc cattctggtt ggtctcgggc
     2761 aagcgcggtg aagaggatga agacgatggc gacgaggaga aatccccgcg ccaggccgaa
     2821 aatgaagccc aaggaccggt ccaaggcgcc gacccggctg tccaggatct tgtcggagat
     2881 ccggaaggtg atcaggctca cgatgatcag cgtgatgatg aaaacgccgg cggcaaacgc
     2941 gatctgggcg atgctcgggt tatcgatata cgggatcagc acctcgtaat agcgcggcgt
     3001 gaaatagagc gccgccacgg cggccatggc ccaggaaaag atcgacagga cttcgcgggt
     3061 gaatccgcga accatcgcca gaaagcccga gatgagcatg atgacgatca aaattatatc
     3121 gagccaggag attggcatgt cgcggacgct ccctcagcca gccggcggca gattatcacg
     3181 attcccggcg atgaagcaca attggccccg tcgggcaatg acccctgggt aacactcgtc
     3241 gcattgtcgc aagggaacgg tctaatcccc gatcaaagcg aaccacgcaa ccagctcatc
     3301 gagttgcgaa accggattca gggcgatccc ctcaacctcg cgctccgcgg aaagagaggc
     3361 ggccggtaca accgcccggg caaagccgag cttggcggct tctttgagcc gctgatcggt
     3421 gtgtccgacg gcgcggacag cgccggtgag gctcacctcg ccgaaataga ccccatcctc
     3481 ggggaccgca cggcccgtaa acgaggagag cagggcggcg gcggcggcga gatctgcggc
     3541 gggttcggaa aggcgcaatc cgccggcgac gttcagatag acatcgtggc tgccaaggcg
     3601 gaccccgcag cgggcctcca gcaccgccag cagcatggag agccggttgg ggtcccagcc
     3661 gaccacagcg cggcgtggag tgccgagtga actcggggcc acaagagcct gtatttccac
     3721 caaaataggt cgagtgccct ccatgcctgc gagaaccgcg gtgccgggcg tcccccggtc
     3781 ggcatcgttg aggaagaggg ccgatgggtt ctccacctct ttgagaccct cagagcgcat
     3841 ctcgaagaca ccgatctcgt cggtggggcc gaagcggttc tttacggcgc gtaggatacg
     3901 gaattggtgg ccgcttaggc tctcgaaata gagcactgtg tcgaccatat gctcgatcac
     3961 tttggggccg gcgatctggc cgtctttggt gacatggccg acgaggacga gggtcgtgcc
     4021 gctggccttg gcatagctga tcagggcgga ggcgcagccg cgcagctgcg agatcgtgcc
     4081 cggcgccgac tcaatgcctt ccgaccacag ggtttgaacg gagtcgatga cgacgagggc
     4141 cggggcgggt ccgctgccga gcgtggcgat gatgctggcg atattggttt cggtgccgag
     4201 cgccacgggg gcttcggcca cgccgagccg gctggcgcgc atgcgaactt gcgcgggcgc
     4261 ttcctccccg gagaggtaga cgatgcggtg accgtcttgg gaaagttcgg cagccgtctg
     4321 aagcaggagc gtggacttac cgatgccggg gtcgccgccc acgagaacgg ccgcgccagg
     4381 cacgagcccg ccgccaagca cgcggtcgag ttcggcaatg tctgtcttga tgcgggaggc
     4441 gccttctgtc tccgcgctca gtgtctcgag actggcgagg cgtcccttgc cgccctttat
     4501 ggccacgagg gccggggaac tcgtgggcgt ggacgcttcc tcgatgatgg agttccaggc
     4561 cccgcaggac gggcactttc ccgtccattt gttcgtgacc gcaccgcagg attggcagac
     4621 ataagctttg gagactttgg ccatgcgttt tcgcgctatc cgccgacgaa acgccggacg
     4681 gcgcggcgcc caaggctggt cagcacctcg tagtcgattg tgttggcgtg gctggcgagg
     4741 tcgtgcgcgc tgatgttgtg cccaagcaat tcgacccatt cgccgcgccg cgcgtgcgtc
     4801 ttgggaacgt ccgtcacgtc gatggcgatc aagtccatgg acacgcgtcc caggatcggg
     4861 gcggcatggt cgccgatgcg gacggccagt ccttcgccgc cgtcggccac ggagagcgcg
     4921 cggaacaacc cgtcggcgta gcccactgaa acaattgcga tgcgtgcgcc gttgggcagt
     4981 gtacgcgtag cgccgtagcc caccgtctcg ccgggctccg cctcgcgcac ctggagaatg
     5041 cgtccgatca ggtgcacgac gggctgaaac ggattgggtc cgcgtcgctg cggatgtccg
     5101 ccgtaaaggg cgatgccggg ccgcaccagg tcgtagacga agtcggcgcc gagcaggatg
     5161 ccggccgagt tggcaaggct cgcccgcgtc tcgggcaatc gcgcacgcaa gctgtcgaag
     5221 atgtcgcgtt gggccgcgtt cttggcgtgc gaagcatcgt cggcgcaggc cagatgactc
     5281 atcaccaaag aaagcgagag cgccgaccaa agctcccggt tgttggacac ggcctcgacg
     5341 tcggctgccg tcagaccgag ccggttcatg ccgctgtcga tatggaccgc gcaagggagc
     5401 gcttcgccgt agcggctgca ttgggcggcc cactcgcgga tttcctcggc gctgttcagg
     5461 acggggcgta ggtctgcttt gcggtagatg tccgccgtcc caggcaggag gccgctgaac
     5521 acatagatca ccgcttcggg cagcagcgcg cgcagctctt ccgcttccgg cagtgtcgct
     5581 acgaagaagg tcttgcagcc ggcgcgccac aaggccggtg cgacttcggt cacaccaaga
     5641 ccgtaggcat cggctttgac gaccgcggcg cattcggccg gcgcggcaag ttcgcgcaaa
     5701 cgatcataat tggcaatcag cgcattgaga tcgatggtga gcagggcggt gctgtccggg
     5761 ggcaccttgt tgtccgcagc ggcgtcgcgc gtgcgcgcgt ggctcactcg ctgcgctccg
     5821 gcagacgctc cgacggggcg agattggaga accgggtgaa ctccggcgtg aactgcagcg
     5881 tcaccgtccc cgtcgggccg tgccgttgct tgccgatgat cacgtcggcc ttgccggtga
     5941 ccgtctccat gtcctgttgc cactgccggt gctcttctgt gttctcgcgg ggctggctcc
     6001 gctccaggta gtattcctca cggaacacga acattaccac atcggcgtcc tgctcgatgg
     6061 agccggattc gcgcaaatca gccagttgcg gccgcttgtc ctcccgctgc tcgacctgac
     6121 gggaaagctg ggagagggcc aggataggca catgcagctc cttagccagg gctttgagcc
     6181 ccgtggtgat ttccgacacc tcctggacgc ggccctcctg cgcgcgccgc gaggagcccg
     6241 acagaagctg gagatagtcg accacgatga agccgagccc acgctggcgt ttcaggcgcc
     6301 gtgcgcgggc ggcgagctgg gccacggtga tgccgccggt ctgatcgatg aacagcggca
     6361 ggttctgaag ctcctgggag acttcgacga tgcggtcgaa ctggtcggag tcgatgcggc
     6421 cgcggcggat gcgctcagag gggatgtagg actgttcgga gatgatgcgg gtggcgagct
     6481 gctccgccga catttccagg gaaaagaagc cgacgaccgc gccatcggcc ggcgtatcgc
     6541 cgcctgcgtt ggcggcgttc gctttggcga cgtggtaggc gatgttggtg gcgagcgccg
     6601 tctttcccag ggcgggacgt ccggcgatga tgatcaggtc cgaggcctga agtccgccca
     6661 tgcgctgatc gaggtcagag aagcccgtag cgatccccga caatccaccg tcgcgctgat
     6721 aggcgttggc ggccatgtcg atggcatcgg tcagcgcggc ggagaagggc tcgaacccgg
     6781 cgccgtactt tcccgtctct gccagttcgt aaagctgctg ctcggcatcc tcgatctggt
     6841 cggagggcgc ggcatcgatc ggagagtcga aggccgtgtg gaccatgcct tcgccgatgt
     6901 cgatgagctg gcgtcgtatg gcgaggtcgt agacggtgcg gccatagtcc tcggcgttga
     6961 tgatggtggt ggcgtttgcc gccagccggc cgagatattg cggcaccggg aggtcggcca
     7021 cgggctcgtc gttctggaag aacgtcttca gcgtgatcgg tgtcgcccgc ttgccgccca
     7081 tgatcaactt ggacgcgctt tcgaagatgc gcccatgaag gcgatcgaag aaatgtccgg
     7141 gcttcaggaa ggtcgagacc cggtctaggg cctcgttgtt gacaaggatg gcacccagaa
     7201 gcgcctgttc ggcctccaga ttgtggggcg actcccggaa aaccggcgtt tcggaggtgt
     7261 cggcagggcg aatcagtgcg gtcatgcgct agctaaccaa aggggcgcgc ctggcagaac
     7321 actgattctc tatgcaagcc tgttattttt ctgtcatgca cagacagtgt tgatatcccg
     7381 ctagcggctg ctccggcgct tgcgctgggg gctcgcgtgg cgttcgcgca ttcgcaactc
     7441 gcgctcggcg tcaaagaaat agtcgcgggt cagaggtagc gcacactgat ccttggtcac
     7501 ctggagctgg aagaccatca ggttctggta gcggaaaccc gtctccgagc ccgccagata
     7561 catttcccac atgcggcaga agcg
//
LOCUS       DBNHVU010000080         9386 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_401757, whole genome shotgun sequence.
ACCESSION   DBNHVU010000080 DBNHVU010000000
VERSION     DBNHVU010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 9386)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9386)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9386
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_401757"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /gene="trpE"
                     /locus_tag="AC16Y7_03025"
     CDS             <1..1449
                     /gene="trpE"
                     /locus_tag="AC16Y7_03025"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIR5708384.1"
                     /translation="WTRLVADLETPVSAMLKVAHEWPNSFLLESVEGGATRGRYSIIG
                     CEPDVIWRSEGNTAEINRSPQADPDAFAPTSESTLASLRALLAESAITLPHQLPPMAA
                     GIFGYMGYDTVRLTEDLPGGKPDPLNVPSSILVRPTLIVIFDSVKDEMTVVTPVRPAQ
                     GVSAKAAYARAADRLNVVLERLDQPLPHGGELGADAPHPEPRSTTPPEDYMAKVARAK
                     EYIAAGDIFQVVLSQRFEAPFELPPFALYRALRRVNPSPFLYYLNFGDFAVVGSSPEI
                     LVRVREREVTIRPIAGTRPRGTTPEQDKALAEELLADTKERAEHLMLLDLGRNDAGRV
                     SEIGSVEVTDSFFLEYYSQVMHIVSNVRGVLAPERDSVDALMAGFPAGTVSGAPKVRA
                     MEIIDELEDAARGIYAGCVGYFSADGEMDSCIVLRTSVVKDGVMYVQAGAGIVADSVP
                     EREQAECVSKAGALFKAADEAVRFAARGKRGQ"
     gene            1467..1988
                     /locus_tag="AC16Y7_03030"
     CDS             1467..1988
                     /locus_tag="AC16Y7_03030"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SixA phosphatase family protein"
                     /protein_id="HIR5708385.1"
                     /translation="MLTLSLLRHAKSSWKNPTLPDRERPLSTRGMSDAPVMGRAMTER
                     GLDPELVLCSSARRTVDTLDLLLPELKVEPEVTYEDALYHASPEDILDMLRAIPPGAS
                     RVLVVGHNPEIEALAGDLIGSGPKHMRDRLKEKYPTGALAVLNFAAGLWSSVDVNSGS
                     LSLFLTPKELRGG"
     gene            2035..2655
                     /locus_tag="AC16Y7_03035"
     CDS             2035..2655
                     /locus_tag="AC16Y7_03035"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR5708386.1"
                     /translation="MLTLIDNYDSFTYNLVHFLGELGASTQVYRNDKVTVDEVLAEGP
                     EAIVLSPGPCDPDRAGICLELIQRAAPVVPILGVCLGHQAIGQVYGGRVIRAPYLMHG
                     KLSKVRHTDKGVFAGLPQEFSATRYHSLILERDTLPEDLEITAETPDGIIMGVQHKRY
                     PVHGVQFHPESIASEHGHQLLANFLDLARVERRQRGNPKREAVGAL"
     gene            2645..3700
                     /gene="trpD"
                     /locus_tag="AC16Y7_03040"
     CDS             2645..3700
                     /gene="trpD"
                     /locus_tag="AC16Y7_03040"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR5708387.1"
                     /translation="MPSNPAPDRAGEPQDLRAALKKVVAGDALTQREAAEAFELIMSG
                     GATDAQIGALLMGMQVRGETVDEITGAARVLRSKALSVEAPEGAIDTCGTGGDAKGTH
                     NISTCTAFVVAGAGVPVAKHGNRSISSRSGSADVLAALGVNLECAPEVIERCISECGL
                     GFMFAPAHHAAMRHVAKVRGELGVRTIFNLLGPLANPAATKYQVVGVFHEKWVEPVAN
                     VLNMLGVERAWVVHGADGLDELTTTDVSHVAALDHGKVSTFKISPRNAGLPDAKPEDL
                     VGGDAVENAAHIRAALQGNQGPLRDIVLLNAAAALLVAGKAKTLQDGVALAADAIDSG
                     RAMNVLEALVKLSHDKV"
     gene            3704..4522
                     /gene="trpC"
                     /locus_tag="AC16Y7_03045"
     CDS             3704..4522
                     /gene="trpC"
                     /locus_tag="AC16Y7_03045"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIR5708388.1"
                     /translation="MAHVLDKILSYKREEIEAAKKTRPESAVRAEAEAAAPVRGFERA
                     LRDRIDHGAFGLIAEIKKASPSKGLIRADFDPPTLAVAYEAGGAACLSVLTDTPSFEG
                     HPEYLTMARAACGLPVLRKDFMIDPYQVFEARVLGADCILIIMAAVDDGLARDLEVAA
                     HGLGMDVLAEVHDDAELDRALKLKTPLIGINNRNLKTFDTTLATTEQLAPRVPSDRLV
                     VAESGIFTHADVTRLAASGVHAFLVGESLMRQADVAAATRALLSGADDTAKAAS"
     gene            4519..4998
                     /gene="moaC"
                     /locus_tag="AC16Y7_03050"
     CDS             4519..4998
                     /gene="moaC"
                     /locus_tag="AC16Y7_03050"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR5708389.1"
                     /translation="MSARLSHLNDKGEARMVDVSEKTVTTRTAIAEGFVSMSPATLDM
                     ILDGTAPKGDVLATARIAGITGAKKTADLIPLCHPLPLTQVTIDFTPSREPCGLRVEA
                     TAKVDGKTGVEMEALTAISVACLTLYDMCKAVDRAMSFSGIRLIEKTGGRSGFYRAP"
     gene            5005..6204
                     /locus_tag="AC16Y7_03055"
     CDS             5005..6204
                     /locus_tag="AC16Y7_03055"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR5708390.1"
                     /translation="MALISVTEAFGRIDSGLDRLGVERVPISDAHGRVLATTLGAKQT
                     QPPFDASAMDGYAVRAADVATLPATLALIGESAAGARFDGVVGPGQAVRIFTGAPVPI
                     GADTIVIQEDTEADGARVVVNAATAGRHIRPRGQDFMEGDKLLEDGTRLGARALMLAA
                     AMNYADVLVRRQPKVAILSTGDEIVPPGDATGPDQIVASVSYGVAALVEDYGGIAIDL
                     GIAPDDEDALVEAISRGSDADILVTIGGASVGDRDLMKTALARAGMALDFAGVAMRPG
                     KPVFYGRLGDQHVLGLPGNPVSAFICSVVFLRTMIGHLLCEDRTAVPHRAVLAEDLPR
                     NGPREHYLRATSSFAEDGTRHVHVLSSQDSSLMASLSKADCLIVQPPHHPGLAKGTKV
                     LVWPLAE"
     gene            6391..7095
                     /gene="lexA"
                     /locus_tag="AC16Y7_03060"
     CDS             6391..7095
                     /gene="lexA"
                     /locus_tag="AC16Y7_03060"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR5708391.1"
                     /translation="MLTPKQKELLMFIHERLKETGVPPSFDEMKEALDLKSKSGIHRL
                     ITALEERGFVRRLPHRARAMEIVRLPDSVPQSVTARARGGFSPSVIEGSLGRVPEVSE
                     GEAAPQTVAVPVMGKIAAGVPAEAIQSRLATIEVPPELLAKGEHYALEVKGDSMIEAG
                     ILEGDTIIVRRCEDATNGDIIVALVDEEEATLKRLRRRGDSVALEAANPSYETRIFGP
                     DRVRVQGRLVGLIRRY"
     gene            complement(7109..7552)
                     /locus_tag="AC16Y7_03065"
     CDS             complement(7109..7552)
                     /locus_tag="AC16Y7_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708392.1"
                     /translation="MALRSVAGDLVFPPATAATYSVENWLLADGDDRDASELAEDEAF
                     RCDPLGCIGRVKGKVVALVRHPGALEEDCRIADILVAPFTVSKTCRAARVLVDRRALK
                     RGGAHALFIDGLSIRTETVTHARRNRPWTRADTHAKTRTRHGLTK"
     gene            7738..9174
                     /gene="gltX"
                     /locus_tag="AC16Y7_03070"
     CDS             7738..9174
                     /gene="gltX"
                     /locus_tag="AC16Y7_03070"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR5708393.1"
                     /translation="MSETVITRFAPSPTGYLHIGGARTALFNWLYAKAKGGKMLLRIE
                     DTDRARSTEGAILAILDGLRWLELDWDGEPVYQFSRAERHREIAERLLAEDKAYRCYA
                     SPEELQAMREAAREEGRAPGYNGLWRHRDPKDAPEGILPAIRFKAPSEGTTLIKDQVQ
                     GEVSFANKDLDDLVLVRSDGSPTYMLSVIVDDHDMGVTHAIRGDDHLTNAARQAQIFG
                     ALGWETPVYAHVPLIHGPDGAKLSKRHGALGIEAYREMGYVPEALRNYLVRLGWSHGD
                     DEIISTDEMIEWFDLDAIGRSAARFDFAKLENLNGHYLRTMADSEIVAQLKALVPVLP
                     DGDAIAARIGESGWAKLEQAMPGLKERAKTLVELLDSADYLFVTRPLTLDDKAKKQLD
                     DTGRETLAALLQELEKVEPWTVEALEGCVRDFAEATDRKLGKVAQPLRAALTGRATSP
                     GIFDVLAVLGRDEALARLSDQSANGHSA"
BASE COUNT         1656 a         2968 c         3064 g         1698 t
ORIGIN      
        1 tggacgcggc tcgtggccga cctggagacg ccggtttcgg cgatgctcaa ggttgcgcat
       61 gagtggccca acagtttcct cttggagtcc gtcgagggcg gcgccacgcg cggccgctac
      121 tcgattatcg gctgtgagcc ggatgtcatt tggcgctctg agggaaacac cgcagaaatc
      181 aaccggagcc cgcaggccga tccggacgcg tttgcgccga cgtctgagtc cacgctcgcc
      241 tcgctccgcg ctttgctcgc ggagtccgcg attaccctgc cgcatcagct gccgccgatg
      301 gcggccggga tcttcggcta tatgggctac gacacggtcc ggttgaccga ggatctgccg
      361 ggcggcaagc ccgacccatt gaacgtgccg agtagtattc tcgtgcggcc gacgcttatc
      421 gtcatcttcg atagcgtcaa ggacgagatg accgtggtga cgccagtacg cccggcgcaa
      481 ggcgtcagcg ccaaggcggc gtatgcgcgg gccgcggaca ggctcaacgt tgttctggaa
      541 cgccttgacc agcccctgcc ccacggtggc gagctgggtg cggatgcgcc tcatcctgag
      601 cccaggtcca ccacgccgcc cgaggactac atggcgaagg tggcgcgcgc caaggagtac
      661 atcgccgccg gcgatatctt tcaggtcgtg ctcagccagc gtttcgaagc gccattcgag
      721 cttcccccgt ttgcgcttta ccgcgcctta cggcgtgtga acccctctcc gtttctctac
      781 tatttgaact tcggcgactt cgccgttgtg ggatccagcc ccgagattct cgtgcgcgtg
      841 cgagaacgcg aggtgacgat ccgaccgatc gcaggcacgc ggccccgcgg cacgacgcct
      901 gagcaagaca aggcactggc cgaagagctc ctggccgaca cgaaagagcg ggccgagcat
      961 ttgatgttgc tcgacctcgg gcgcaacgat gccgggcgtg tatcggagat cgggtccgtc
     1021 gaagtcaccg acagtttctt cctcgagtat tacagccaag tcatgcacat cgtttctaat
     1081 gtccgcggcg tgctcgcccc ggaacgcgat tccgtcgatg cactgatggc gggcttcccg
     1141 gccggcaccg tatcgggtgc gccaaaggtg cgtgcgatgg agatcattga cgagctggaa
     1201 gatgcggcgc gcggcattta tgcgggctgc gtcggttact tctccgccga tggcgagatg
     1261 gacagttgca tcgtgctgag gacgtcggtg gtaaaagacg gcgtcatgta tgttcaggca
     1321 ggcgccggta ttgtcgccga ctccgtgccg gagcgggagc aagccgaatg tgtcagcaag
     1381 gccggcgcgc tgttcaaggc cgcggacgag gccgtgcggt tcgccgcccg tggcaagcgc
     1441 ggccagtaac cgggtagagg ccggacatgc tcacactcag cctcctgcgc catgccaagt
     1501 cgagttggaa gaaccccact ttgccggatc gggaacggcc cctttccacg cgcggcatga
     1561 gcgatgcacc ggtcatgggg cgcgccatga cagagcgggg tctcgacccg gagcttgttc
     1621 tgtgctcttc ggcgcgccga accgtcgata cgctggacct gctcctgccg gagctgaagg
     1681 tcgagccgga agtcacctat gaggacgcgc tttaccacgc gagcccggag gacatcctgg
     1741 atatgctgcg ggcgatcccg ccgggggcga gccgcgttct ggtggtgggt cacaaccccg
     1801 agatcgaggc gttggcaggg gacctgatcg gctccgggcc gaaacacatg cgcgaccgtc
     1861 tgaaggagaa atacccgacc ggcgcgctcg cggtgctcaa cttcgccgcc gggctgtggt
     1921 cgagcgtcga cgtcaatagc ggcagcctga gcctgttcct cacgcccaag gagctgcgcg
     1981 gaggctagcc cctcgacctt gacgtcagct aggggccggg cgtaacacca agccatgctg
     2041 accctgatcg ataactacga cagcttcacc tacaacctcg ttcatttcct tggggaattg
     2101 ggcgcgagca cgcaggtcta ccggaacgac aaagtgaccg tggacgaggt gctggcggaa
     2161 gggcccgagg ccatcgtgct gtcgccggga ccttgcgatc cggaccgggc cggcatctgc
     2221 ctggagctga tccagcgcgc cgcccccgta gtgccgatcc tcggcgtttg cctcggtcat
     2281 caggccatcg gtcaagtcta tggcggccgc gtgatccgtg cgccgtatct catgcacggt
     2341 aagctgtcca aggtgcgcca tacggacaag ggcgtgtttg ccggactacc gcaggagttc
     2401 tccgcgacgc gctatcattc gctcatcctg gagcgcgaca cgctgccgga ggatctggag
     2461 atcaccgcgg agacgccaga cggcatcatc atgggcgtgc agcacaagcg ctatcccgtg
     2521 catggcgtgc agttccatcc cgaaagcatt gcctcggagc acggccatca attgctcgcc
     2581 aacttcctcg atctcgcgcg cgtcgaacgg cgtcagcgcg gaaacccaaa gagagaagcg
     2641 gtcggtgccc tctaatcccg cgccggatcg cgccggcgag cctcaagatt tgcgcgcagc
     2701 cctcaagaag gtggtggcgg gcgatgccct gacacagcgg gaggccgccg aggccttcga
     2761 gctcatcatg tccgggggcg cgaccgacgc gcagatcggg gcgctgctga tgggtatgca
     2821 ggttcgcggc gaaacggtcg acgagatcac cggtgccgcg cgcgtgctcc ggagcaaggc
     2881 gctgtcggtc gaggcgcccg agggcgccat cgacacatgc gggaccggcg gggacgccaa
     2941 aggcacgcat aacatctcga cctgcacggc ctttgtcgtt gcgggcgcgg gcgtgcctgt
     3001 ggccaagcac ggcaatcggt cgatctcttc gcgctcgggc tcggccgatg tgctcgcggc
     3061 ccttggtgtc aatctcgaat gcgcgcccga agtcatcgaa cgctgcatct cggagtgcgg
     3121 gctgggtttc atgtttgccc ctgcccatca tgcggccatg cgccacgtcg ccaaggtgcg
     3181 cggcgagctt ggcgtgcgga cgatctttaa tttgctcggg ccgttggcga acccggcggc
     3241 caccaagtac caggtcgtcg gcgtcttcca tgaaaaatgg gtagagcccg ttgcgaacgt
     3301 cctgaacatg cttggtgtcg agcgcgcttg ggtggtgcac ggcgccgatg ggcttgacga
     3361 actgaccacc acggatgtca gccatgtggc ggctctggat cacggcaagg tctcgacgtt
     3421 caagatttcg ccccgtaatg ccggcctacc ggacgcaaag ccggaggatc tcgtcggcgg
     3481 tgatgccgtg gagaacgccg cgcatatccg ggccgcgctg caaggcaatc aaggtccgtt
     3541 gcgcgacatc gtgctgttga atgccgccgc cgcgctcctc gttgccggca aagccaagac
     3601 tttgcaggat ggtgtggcgc ttgccgcgga cgccatcgat agtggccggg ccatgaacgt
     3661 cttagaggcg ctggttaaac tgagccatga taaggtctga gccatggccc acgtgctaga
     3721 caaaatactt agctataagc gcgaagagat tgaagcagca aagaaaacac ggcctgagag
     3781 tgccgtgcgc gcggaggctg aagctgccgc gccggttcgc ggcttcgaac gcgccttgcg
     3841 ggaccgcatc gatcatggcg ccttcgggct gattgcagag atcaagaagg cgagcccgtc
     3901 aaagggattg atccgcgccg atttcgatcc gcccaccctc gccgtcgcct acgaggcggg
     3961 cggcgctgcg tgcttgtccg tgctgaccga tacgccctcc ttcgaggggc atccggaata
     4021 tctcaccatg gcgcgcgcgg cctgcggcct ccccgtcttg cgcaaggact tcatgatcga
     4081 tccgtatcag gtgttcgaag cgcgcgtgct tggggctgac tgcattctca tcatcatggc
     4141 ggcggtcgac gatgggctcg cgcgcgactt ggaagtcgcc gcgcacgggc tcggcatgga
     4201 cgtcctcgcc gaggtgcatg acgatgccga actcgaccgg gcgctaaagc ttaagacgcc
     4261 gctcatcggc atcaacaatc gcaatctgaa aaccttcgac acgacgcttg cgacgacgga
     4321 acagctcgcg cctcgcgtcc cttccgatcg tcttgttgtc gcggagagcg gaatcttcac
     4381 ccatgcggat gtgacgcgcc ttgcggcaag tggtgtgcac gccttcttgg tcggcgagag
     4441 cttgatgcgg caggctgacg tggcggctgc cacgcgggcc ctgctctcgg gcgcggatga
     4501 caccgccaag gcggcatcat gagcgcgcgg ctgtcccacc taaacgacaa gggcgaggcg
     4561 cgcatggtcg acgtctcgga gaagaccgtc acgacgcgta ccgctattgc cgaaggcttc
     4621 gtgtcgatga gccctgcgac gcttgacatg atcctcgacg gcaccgcccc caaaggcgac
     4681 gtgctagcca cggcacgcat cgcagggatc acaggcgcca agaagaccgc cgatctgatt
     4741 ccgctgtgcc atccgctgcc cctcacccag gtcaccatag attttacgcc ttcgcgcgaa
     4801 ccctgcggcc tccgcgtcga ggcgaccgcg aaagtcgacg gcaagacggg tgtggagatg
     4861 gaggctttga cggcgatctc ggtggcctgc ctcacgctct acgacatgtg caaggcggtg
     4921 gaccgcgcca tgagcttctc cggcatccgc ttgatcgaaa agaccggcgg ccgctccggc
     4981 ttctatcgcg cgccctgacc ggcgatggcc ctcatctccg taaccgaggc gtttgggcgg
     5041 atcgattcag gtcttgatcg gctcggcgtc gagcgggttc caatctcgga cgcccatggt
     5101 cgcgtgttgg cgaccacctt gggcgcgaag cagacacagc ccccgttcga tgcctcggcc
     5161 atggacggct atgcggtgcg cgccgccgat gtggccaccc tgcccgcgac gctggcgctg
     5221 attggcgaat ccgccgcggg cgcgcggttt gacggcgttg tcggaccggg ccaggctgtc
     5281 cgcatcttca ccggcgcgcc cgttccgata ggcgccgata ccatcgttat ccaggaggac
     5341 acggaggcgg acggcgcccg cgttgtcgtg aatgccgcga cggccggccg tcatatccgc
     5401 ccgcgcggtc aggacttcat ggaaggcgat aagcttctag aggacggcac ccgccttggg
     5461 gcgcgggcgt tgatgctggc ggcggcgatg aactacgcgg acgtgctcgt acgccggcag
     5521 ccgaaggtcg ccatcttatc gacgggcgat gagatcgtgc cgcccggaga cgcaacgggc
     5581 cccgatcaga tcgtggcctc ggtgagctat ggcgttgccg cgctcgttga ggactatggc
     5641 gggatagcga tcgacctggg gatcgcccca gacgatgagg acgcgctggt ggaggccatc
     5701 tcgcgggggt ccgacgccga tattctcgtg accatcggcg gtgcgtccgt tggggaccgc
     5761 gatctgatga agacggctct cgcccgcgcc gggatggcgc tggacttcgc cggggtcgcc
     5821 atgcggcccg gcaagccggt gttctatgga cgtctgggag accagcacgt gctcggtctt
     5881 ccaggaaacc cggtctcggc ctttatctgt tcggtggtgt tcctgaggac gatgattggc
     5941 catcttctct gcgaggatcg caccgcggtt ccgcatcgcg cggtcctcgc cgaggatctg
     6001 ccgcggaacg gtccccgtga gcattacctg cgggcgacct ccagctttgc cgaggacgga
     6061 acgcgccacg ttcacgtcct gtcctcccag gattcgtcgc tcatggcctc attgtccaag
     6121 gccgactgcc tcatcgtgca gcccccgcac caccctggcc tggccaaagg cacaaaggtc
     6181 ctggtttggc ccttggcgga atagttaagg ccccagaaac ccgaatcacg cgagcaaatg
     6241 tccgccagac cttctggcaa aacacatttt gtggccatgc agcttgcgga acacaactag
     6301 aacatctata ctgttcatgg tttgtacagg cggggcgaga atcgtttcta ggcgttacag
     6361 cccgattcct gatcgctcga aggggatttg atgctcacac cgaagcagaa agagcttctc
     6421 atgttcatcc acgagcggtt gaaggaaacg ggcgtgccgc cctccttcga cgagatgaaa
     6481 gaggcgctcg atctcaagtc gaaatccggc attcacaggc tgatcacggc cctggaggag
     6541 cgcggcttcg tgcgccgcct gccccaccgg gcccgggcca tggaaatcgt ccgccttccg
     6601 gactccgtgc cgcagagcgt cacggctcgt gcgcgggggg gcttctcccc cagcgtgatc
     6661 gaaggcagct tggggcgtgt tcctgaggtc tccgagggcg aggcggcgcc acagaccgtc
     6721 gctgtacccg tcatgggcaa gatcgccgcc ggcgtccccg ccgaggccat ccaaagccgg
     6781 cttgccacca tcgaggtgcc gccggagctg ctggccaagg gcgagcacta tgcgctcgag
     6841 gtcaaaggtg actccatgat cgaggccggg atcttggaag gcgacaccat catcgtgcgc
     6901 cgctgcgagg acgccaccaa cggcgatatc atcgtggcgc tggtggacga ggaggaagcc
     6961 acgctcaagc gtctacgccg ccgtggggat tccgtcgcgc tcgaggccgc caacccgtcc
     7021 tacgagaccc gcatattcgg acccgatcgg gtgcgcgtgc aagggcgcct tgtcgggctc
     7081 atccgccgct actaggaccg cttccctctt atttcgtcag tccgtgccgc gtgcgcgtct
     7141 tcgcgtgtgt gtcagcccgc gtccaggggc ggtttctgcg cgcatgggtt accgtttcgg
     7201 tgcggattga gagcccgtct atgaaaagcg catgcgcgcc gcctcgcttc agcgcgcgcc
     7261 ggtcgacgag aacccgcgcg gcgcggcagg tcttcgacac ggtgaacggc gcgacgagga
     7321 tgtcggcgat gcggcagtcc tcttccagcg cgccgggatg gcgcaccagg gcgacgacct
     7381 tgcccttgac ccggccgatg cagccgagcg gatcgcagcg gaaggcttca tcctcggcaa
     7441 gttcgctagc gtcccggtcg tcgccgtccg ccaggagcca gttctccacg ctataggtcg
     7501 cggcggtcgc cggcggaaag acgaggtcgc cggccactga gcgcagcgcc acggtggccc
     7561 cttccctctc gatcagaacg tccgggcgat gttgtgcggc cgcccccgct ctcggcgtcg
     7621 ttctcctgga ccggcatggg acccccgccc caaacacccg cttacccgca cctaagccct
     7681 atgctacacc acgcccaacc ttgtccccac cagctttgag accttccaga tcgatctatg
     7741 agcgagacag tcatcacgcg ctttgcgccg tcccccaccg gctacctgca tattggtggc
     7801 gcccgcacgg ccctgttcaa ctggctctat gccaaggcga aaggcggcaa gatgctgctt
     7861 cggatcgagg acaccgaccg ggcgcgctcc acggaaggcg cgatcctcgc gattctggac
     7921 ggcttgcgct ggctggagct cgattgggac ggggagccgg tctaccagtt ctcccgcgcc
     7981 gagcgtcatc gtgagatcgc cgagcgcttg ctggccgaag acaaggccta tcgttgctat
     8041 gccagccctg aggaacttca agcgatgcgc gaggcggcgc gcgaagaagg ccgtgcgccc
     8101 ggctataacg gcttgtggcg tcaccgcgat ccgaaggacg cgcctgaagg gatcttgccc
     8161 gcgatccgtt tcaaagcacc gagcgaaggc acgacactca taaaggacca ggtccaaggc
     8221 gaggtcagtt tcgccaacaa ggatctcgat gatctcgtac tggtgcggag tgacggctcg
     8281 cccacctata tgctgtctgt gatcgtcgac gaccatgata tgggcgtgac ccatgctatc
     8341 cggggcgacg atcacctgac caatgccgcg cgtcaggcgc agatcttcgg tgcgcttggt
     8401 tgggagacgc cggtttacgc acatgtgccg ctgatccacg gaccggacgg cgcgaaacta
     8461 tcaaagcggc acggcgcgct tgggatcgaa gcgtatcggg agatgggcta cgtgccggag
     8521 gccttgcgca actatcttgt gcgcctcgga tggagtcacg gcgacgatga gatcatctcg
     8581 acggacgaga tgatcgaatg gttcgacctc gatgcgatcg gccggtcggc tgcgcgcttc
     8641 gatttcgcca agctcgaaaa tctcaacggt cattatctgc gcacgatggc ggacagcgag
     8701 atcgttgcgc agctcaaagc gttggtgcct gtgctgcccg acggtgatgc gattgcggcc
     8761 cgcatcggtg aaagcggttg ggcgaagctg gaacaagcca tgccgggctt gaaggaacgc
     8821 gccaaaacgc tggtcgagct tctcgacagt gccgactacc tctttgtcac ccgtcctctg
     8881 acgctggatg acaaggcaaa gaagcagctc gacgatacgg ggcgggaaac gcttgccgcg
     8941 ctgttgcaag agctggagaa ggtcgagccg tggaccgttg aggcgctgga aggttgcgtg
     9001 cgtgactttg cagaggcgac ggaccggaag cttggaaagg tggctcaacc tttgcgcgcc
     9061 gcgctgaccg gccgtgcaac ctcccctgga atcttcgatg ttctggcggt tttgggccgg
     9121 gacgaagcgc ttgcgcgcct ttctgaccag tcagccaacg gccatagtgc ttaacatttg
     9181 ggcaggcgcc tgtgttgcac tgcggtagaa tccccgcgat tattcgcagc gcaaaaaatc
     9241 tgtttccaag gacccaagaa ccgtgcctga agtgatatgt cgtacgcacg tttggtccga
     9301 ttgcccccaa aggggccagg aggttttgag atgagtgtcg cagaggactt cccgcccgcc
     9361 aaaacggacg aggcttccct caaact
//
LOCUS       DBNHVU010000081         4246 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1071068, whole genome shotgun sequence.
ACCESSION   DBNHVU010000081 DBNHVU010000000
VERSION     DBNHVU010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1071068"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1149)
                     /locus_tag="AC16Y7_03075"
     CDS             complement(79..1149)
                     /locus_tag="AC16Y7_03075"
                     /inference="COORDINATES: protein motif:HMM:NF012245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR5708394.1"
                     /translation="MNQSLKDLSNVILRFTKWDMDGEVPHAKRSDEIGVIAKALKAFQ
                     VDAIQWSESHKSEQDSQIQGRLASQQRTEELIHQFRGSIAGILGAFADSARQMDETAR
                     ALSTVASDTNERVTSVASASDEASANVQTVAATAEELAVSVGDIGERVSTASRIVDDV
                     TENARAANNKVEGLASSAQRIGDVVSLIQDVAAQTNLLALNATIEAARTGEAGRSFAV
                     VASEVKTLADQTAKATEDIKHQIDTIQSSTNGAVDAMQSIVTTMGDVNKNTQEIAGAV
                     QQQSAATSEISHSVRQAARGTEDVVAHMPGVTQAVDETSQSATQMLQVSRDLSRQAEQ
                     LRHTVENFLRQVAAADALKRAS"
     gene            complement(1166..1321)
                     /locus_tag="AC16Y7_03080"
     CDS             complement(1166..1321)
                     /locus_tag="AC16Y7_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708395.1"
                     /translation="MVLRLKITLAYAATALMAVIVSFGLLTMFDSPGLAAVLALIIAM
                     GLGAGYG"
     gene            complement(1499..3544)
                     /locus_tag="AC16Y7_03085"
     CDS             complement(1499..3544)
                     /locus_tag="AC16Y7_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HIR5708396.1"
                     /translation="MQSQWNDDTAKETVKAYGAEGVNEDIALRVYTTRLIGRDPLLVL
                     HGGGNTSVKTSATDDLGQAHEVIAVKGSGADMADMEPWGLPEVKLGPLRETRSLDALS
                     DEAMVNVQRLNLLNASAPNPSVETLLHAFLPHKFVDHTHAAAVLSLVDQPDGEALARE
                     VYDGRMGIVPYIAPGFALAKAAADVFDEKPDVEGLILHKHGIFTFGETAREAYERMIE
                     MVSLAESRLRQGRPVVFQKAKTPAKLEAAAEIAPILRGACAVRKDGAEPVRFIAELRN
                     SPEILDYVNGAELGSYSQRGVVTPDHIIRTKNTPLFVPAPEAGKLEAFARDVKAAVEN
                     FAADYDAYFARENAAVGGIKTKLDPMPRVILVPGVGLFGLGRNAKAAGIAGDLAENTI
                     KVVTDAEAIGRYDPLPERDLFALEYWSLEQAKLKAQVEKPLTGQVAVVTGAGAIGAAT
                     AKAFAGDGAAVAVLDIDGEAAKSAAAQVKGLGLACDVTKPDQVTRAFAEICERYGGVD
                     ILVSNAGAAWQGRIGEVADETLRQSFELNFFAHQTVAKAAVQVMLKQGTGGALLFNIS
                     KQAVNPGQNFGPYGLPKAATMLLMRQYALDYGADGIRSNGVNADRIRSGLLTAEMIAA
                     RSKARGLSEADYMSGNLLHAEVLADDVAQAFLSLAKARKTTGHVETVDGGNIAAALR"
     gene            3705..3941
                     /locus_tag="AC16Y7_03090"
     CDS             3705..3941
                     /locus_tag="AC16Y7_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708397.1"
                     /translation="MGSFRNAYRPERHYMRGPGPKWHAKHTASLIAAAGVQPIMAANA
                     PHAHAGPGLNIVMSWIAPTAAAAALGFVAVMMLA"
BASE COUNT          739 a         1396 c         1291 g          820 t
ORIGIN      
        1 tgcgcgcaag tttgcatcaa acgcaaatgg ccgggtcgaa acccggccac cgacgcggaa
       61 cgaattctcg aatttaagtt agctggcgcg cttcagagca tcagccgctg cgacctggcg
      121 caagaagttc tccaccgtgt ggcgaagctg ctcggcctgg cgcgacaggt cacggctgac
      181 ctgaagcatc tgcgtcgccg actggctggt ctcgtccacg gcctgcgtga cacctggcat
      241 atgcgccacg acgtcctcgg tgccgcgcgc ggcctgacgc acgctgtggg aaatctcgga
      301 ggtcgcagcc gattgctgct gcacggcgcc tgcgatctcc tgggtgttct tgttcacatc
      361 gcccatcgtg gtgacgatgg actgcatcgc atccaccgcg ccgttggtcg acgattggat
      421 ggtatcgatt tggtgcttga tgtcttcggt ggctttggcg gtttggtcgg cgagtgtctt
      481 cacctcactc gccacaacgg caaagctgcg tcccgcctca cctgtgcgcg ccgcctcgat
      541 cgtggcgttc agcgcaagaa ggttcgtctg cgccgcgaca tcttggatga gactcaccac
      601 atcgccgatg cgctgggcgg aactcgccag cccctccacc ttgttgttgg cggcacgggc
      661 gttctcggtc acgtcgtcga caatgcgact ggccgtggag acccgttcgc cgatatcgcc
      721 gaccgagacg gccagctcct cggcggtcgc ggcgacggtt tgcacattgg ctgacgcctc
      781 gtcggaggca gaggccacgg aggtgacccg ctcatttgtg tcggaggcga cggtcgacag
      841 agcccgcgcc gtctcgtcca tctgacgtgc gctgtcggca aaggcgccga ggataccggc
      901 gatcgagccc cggaattgat ggatcagttc ttcggtgcgc tgctgcgagg caagccgccc
      961 ttggatctgg ctgtcctgct cgctcttatg gctctcgctc cattgaatgg cgtcgacctg
     1021 gaaggccttc agggccttgg cgatgacacc gatctcatcg gaacgcttgg cgtgcggcac
     1081 ctcgccgtcc atgtcccact tagtgaagcg caagatgacg ttgctgaggt ccttgagcga
     1141 ctggttcaac gcaccgcccc agtaattagc cgtacccggc gccaagcccc atcgcgataa
     1201 tcagcgccaa gaccgcggcg agcccggggc tgtcgaacat ggtcaggagc ccaaacgaga
     1261 caataacagc catcagtgcc gtggcggcgt aggcaagcgt gatcttcaac cggagaacca
     1321 tttgaaatct atcaatattt aagacgctaa gaaccgtccg aaagcctgcc agaacggctc
     1381 gatgaagcga atcccccaac aggcgtgtcg aggagaagcc tctccggttg tggttaaaac
     1441 tcccttagga ttgccctcgt ttgggcacgt ccaaagaaaa atttcgagga atccggccct
     1501 agcggagcgc cgcggcgatg ttgccgccgt ccaccgtctc cacgtgaccc gtcgtcttgc
     1561 gggccttggc caagcttaag aacgcctgag ccacatcgtc cgccaggacc tcggcgtgga
     1621 gcagattgcc gctcatatag tcggcctcgg acagcccccg cgcctttgag cgcgccgcga
     1681 tcatctcggc cgtcaggagc ccgctgcgaa tacggtcggc attgaccccg ttggagcgga
     1741 tcccgtccgc gccataatcg agcgcgtatt ggcgcatgag cagcatggtg gcggccttcg
     1801 gcaatccata tggcccgaaa ttctgccccg ggttcaccgc ttgcttggag atgttgaaca
     1861 gaagcgcccc gccggtgcct tgcttcagca tcacctggac cgcggctttc gccaccgttt
     1921 ggtgcgcgaa gaagttgagc tcgaagctct ggcgcaaggt ctcgtccgca acctcgccga
     1981 tgcgtccttg ccaggcggcg ccggcgttcg acacgagaat gtcgacaccg ccatagcgct
     2041 cgcagatctc ggcgaacgcg cgcgtcactt ggtccggttt cgtcacgtcg caggcgagac
     2101 caagcccttt gacttgggcc gcagcgcttt tcgccgcctc gccgtcaatg tcgagcacgg
     2161 caaccgccgc gccgtcgccg gcgaatgcct tggccgtcgc cgcaccgatg gcaccagcgc
     2221 ctgtcaccac agcgacttgc cccgtgagcg gcttctccac ctgcgctttc agcttggctt
     2281 gctccaggct ccaatattcg agcgcgaaga gatcgcgctc cggcagcggg tcgtagcgcc
     2341 cgatggcctc cgcatcggtc accaccttga tcgtgttttc ggcaagatcg cctgcgatgc
     2401 ctgcggcctt ggcgttacgc ccaagtccga acaggccgac gcccggcacg agaatgacgc
     2461 gcggcatcgg gtcgagcttg gtcttgatgc cgccaacggc cgcgttctcg cgcgcgaaat
     2521 aggcgtcgta gtcggcggca aaattctcca ccgccgcttt cacatcacgc gcaaacgctt
     2581 cgagcttgcc ggcttccggc gccggcacga acaacggcgt gttcttggtg cgaatgatgt
     2641 ggtcgggcgt caccacgccg cgttggctgt acgagccaag ctccgctccg ttcacgtagt
     2701 cgagaatctc cggactgtta cgcagctcag caatgaagcg gacaggctcg gcgccgtctt
     2761 tgcggactgc gcacgcaccg cgcaggatcg gcgcgatctc cgctgccgct tccagcttcg
     2821 ccggcgtctt cgccttttgg aacacgacgg ggcgtccctg ccgcaaccgg ctttcggcga
     2881 gcgacaccat ctcgatcatg cgctcatagg cttcgcgcgc cgtctccccg aacgtgaaga
     2941 tgccgtgctt gtgcaggatc agcccttcga cgtcgggctt ctcgtcgaac acgtccgcgg
     3001 cggctttggc cagcgcgaag cccggcgcga tatagggcac gatacccatg cgcccgtcat
     3061 agacctcgcg cgccaacgcc tcgccgtcgg gctgatcgac cagcgacagc actgcggcgg
     3121 catgggtgtg gtccacgaat ttgtgcggca gaaaggcgtg aagcagcgtc tccacggacg
     3181 ggttgggcgc cgaggcgttc agcaggttta gccgctgcac attgaccatc gcctcgtcgg
     3241 acaacgcatc gaggctgcgc gtctcccgca agggtccaag cttcacctcc ggcaatcccc
     3301 acggctccat gtcggccatg tcggcgcccg agcctttgac cgcgatgacc tcgtgcgcct
     3361 ggccgagatc gtcggtcgcg ctggtcttca ccgaggtgtt gccgcctcca tgcaggacaa
     3421 gcaggggatc acggccgatc aggcgcgtgg tgtagacgcg aagggcaatg tcctcattga
     3481 cgccctcggc gccataggcc ttgaccgtct ctttcgcggt gtcgtcgttc cattggcttt
     3541 gcatgtcgcg ggttttggca agtttcgtga ggcgcggcaa gaacggagcc gccccggccc
     3601 ttgcggcaaa gaatcctcat tttcgccccg gttgcatacc gattggcaac atattcgtat
     3661 gaagattaag acgttagggg ctcatatgga tgagggattg caaggtgggt agttttcgta
     3721 acgcgtaccg gcccgaacgc cactatatgc gtggcccagg gccgaaatgg cacgccaagc
     3781 acacggcctc gctcatcgcc gctgcgggcg ttcagccgat catggccgcg aatgcgcctc
     3841 acgcgcacgc cgggccgggc ctaaacatcg taatgtcctg gatcgcgccg acggccgccg
     3901 ccgccgcgct aggcttcgtc gccgtcatga tgctggctta agccacacat acgctgagac
     3961 ggcttggggc gttccgcctg gatcggtggg attcactgat ctgaaaccgg aaagccccaa
     4021 aacagtggaa ttcatgggtt cctttttggg tgcccaatcc ccacttttag ggttcgccga
     4081 gggagaaaac ccaagcacgc gaggtaagca ctcatgcgca cagtacgtct cacggccctt
     4141 tcaggcctcc tggccgtctt cgtcgtcgcg agcccccagc ccgcctcggc aaatcccgcg
     4201 gccgcggccc tgaaactcaa ccacgccaac accgccgacg tgattc
//
LOCUS       DBNHVU010000082         4468 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_775966, whole genome shotgun sequence.
ACCESSION   DBNHVU010000082 DBNHVU010000000
VERSION     DBNHVU010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_775966"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /locus_tag="AC16Y7_03095"
     CDS             <1..177
                     /locus_tag="AC16Y7_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR5708398.1"
                     /translation="FTAKDDNLPPRLVSEPAQTGPAKGLVNGLEKMLPEYYQLRGWTT
                     DGVPTNETLSRLAL"
     gene            283..1593
                     /locus_tag="AC16Y7_03100"
     CDS             283..1593
                     /locus_tag="AC16Y7_03100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5708399.1"
                     /translation="MKYVVLGAGPAGVTAAETIRGVDKSGEITLIGGEPEPPYSRMAI
                     PYLLHGNIEERGTYLRQDDNHYNNLGIGYKTGRAGGIDTRNKVLYMSGGEEIAYDKLL
                     LATGASPIIPPIPGSNLAGVHNCWTLEDARAILKLADRGAPVVLVGAGFIGSIVLEAL
                     YQRGCDLTVVEIAPRMVARMMDETAGGMLGRWCLDKGVKVVTDTRVEAISQATGDGDA
                     GLSVKLSSGQSVPAKLVVLAAGVRSNVGFLVGSGINVGQGITVSETLETTASDVYAAG
                     DCCEGIDLSTGKPDMLAIQPVAVEHGRYAAMNMAGRYTPHRGSLNMNVLDTMGLISSS
                     FGLWQGHPDGKGTRLTDEKAYKYLKLEFLDDKLVGAQCVGLTDHVGMLRGLIQTGLRL
                     GEWKDKLLKSPERLREAYVAVGQTGQMTGPMGPRTNMPVAAEAA"
     gene            1757..2008
                     /locus_tag="AC16Y7_03105"
     CDS             1757..2008
                     /locus_tag="AC16Y7_03105"
                     /inference="COORDINATES: protein motif:HMM:NF025807.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR5708400.1"
                     /translation="MTVTLKLYASLGAFLPEGAEKNVAQVPLQDGDTIETLINRYNVP
                     PEACHLVLLNGIFQPPGRRGEARLKAGDAVAVWPPVAGG"
     gene            2059..2316
                     /locus_tag="AC16Y7_03110"
     CDS             2059..2316
                     /locus_tag="AC16Y7_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708401.1"
                     /translation="MTAREFADGCERLADAGDEVSFSLATFAVSNGQVAVRFEPLDGV
                     TLGGLLKLPRARVTLTFSGLSDQERTDFLRRFDMAFQRGGG"
     gene            complement(2341..4452)
                     /locus_tag="AC16Y7_03115"
     CDS             complement(2341..4452)
                     /locus_tag="AC16Y7_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR5708402.1"
                     /translation="MTDLSDRASQHPPAAIQHPTERTYHSHKLEDPYTWLRAGNWQEV
                     MRDTSKLPSDIRTHLEAENAYTAAVLAPTQKLQAELFEEMKARIKEDDKSVPDPDGPF
                     AYMYDFITGGQYPRLLRLPQDGDETTVLLDGNKEAEGKSYWDLGASAHSPNHRYLAYS
                     FDDKGSELYTIRIRDTETGEDLPDIITDTRGSLQWANDNRTLFYVRVDGNHRPLYVYR
                     HTLGTPVSDDALVYEEKDHGFYVGIEKALSQKFILIDAHDHQTSEIRLLDADAPASPP
                     RLIAARRHNHQYDVCHRGDDLIILTNSDGAEDFRICTAPVCDPSEKNWTTLQPHKPGR
                     LILEMEIYRDFDVRLERENSLPRLVIRNAASGDEHAIAFDEEAYALGMQSGYEFDTAT
                     LRFTYSSMTTPAETYDYDMRTRDRVLRKRREVPSGHNPSDYVTRRLYAPAEDGETVPV
                     SLLYHKDTPLDGSAPLLLYGYGAYGISIPASFVANRLSLVNRGFIYAIAHIRGGKDKG
                     YRWYTDGKLSRKTNTFTDFIAAGEHLVAEGYTRRKHIVAHGGSAGGMLMGAVANMAPD
                     LFLGIIADVPFVDVLNTMLDDTLPLTPPEWLEWGNPIKSAEEFERIRSYSPYDNVERK
                     AYPHILAMTGLTDPRVTYWEPAKWVARLREKNTSGNYILLNTNMEAGHGGASGRFSQL
                     KEVAIEYAFALLITGQAANNV"
BASE COUNT          832 a         1172 c         1504 g          960 t
ORIGIN      
        1 tttacagcca aggatgacaa cttgccgccg cgtcttgtca gcgagccggc gcagactggt
       61 ccggccaagg ggcttgtcaa tggcctcgag aaaatgctgc cggagtatta tcagcttcgc
      121 ggctggacga cagacggcgt accaaccaac gaaacgctgt cacggctggc gctgtaaccg
      181 ggtggaaaag acccgttgaa acaatcgggg cagccgggcg cactgacatg atcgccgggc
      241 tgccccgttc cttcatataa gtccctcaag aggatggtcg atatgaaata tgtggttctt
      301 ggtgcgggtc cggccggtgt gacggcggct gaaaccattc gcggcgtcga taagtcgggt
      361 gagattacgt tgatcggcgg tgagccggag ccaccttatt cgcgcatggc cattccgtat
      421 ctcctgcacg gcaacattga agagcggggt acgtacctgc gccaggacga caaccactat
      481 aacaacctcg gcattggcta caagaccggc cgcgcgggcg gcatcgatac ccgcaacaag
      541 gtgctttaca tgtcgggtgg ggaagagatt gcctacgaca agctgttgct tgcgacgggt
      601 gcttcgccga tcattccgcc gatcccgggt tcgaaccttg caggcgtgca caattgctgg
      661 acgcttgagg atgcgcgggc gatcttgaaa ctggctgacc gaggggcgcc ggtggtgctg
      721 gttggcgccg ggtttatcgg ttcgatcgtt cttgaggcgc tttaccagcg cgggtgcgat
      781 ttgacggtgg ttgaaattgc accgcggatg gtggcgcgca tgatggacga gaccgctggc
      841 gggatgcttg gccgctggtg cctcgacaaa ggcgtcaagg ttgtcaccga cacccgcgtc
      901 gaagcgattt cccaggccac gggcgatggc gatgcgggcc tctcagtgaa gctgtcaagc
      961 ggtcaatcgg tgccggcgaa gctcgtcgtg ctggccgcgg gcgtgcgctc gaatgttggt
     1021 ttccttgtcg ggtctggcat caatgtcggc cagggcatca cggtttcgga gacgctcgag
     1081 acaacggcat cagatgtgta tgcggcgggc gattgctgtg aaggcatcga cctctcgacg
     1141 ggcaagccgg acatgctggc gatccaaccg gtggcggtcg aacatgggcg ctatgcggcg
     1201 atgaacatgg ctggccggta tacgccccac cgcggctctc tcaacatgaa cgtcctcgat
     1261 accatgggct tgatctcgtc ctcgttcggc ctctggcagg gccatcccga cggcaagggc
     1321 acgagactga cggatgaaaa agcctacaag tatctgaagc tcgaattctt ggatgataag
     1381 cttgttggcg cgcaatgcgt ggggttgacc gatcatgtcg gcatgctgcg cgggttgatt
     1441 cagaccgggc tcaggcttgg tgagtggaag gacaagcttc tgaaatcgcc ggagcgtttg
     1501 cgtgaagcct atgtggcggt cgggcaaacc ggtcagatga cagggccgat gggtccacgg
     1561 accaatatgc cggtggcggc ggaagcggct tgacgccggg tcccaaccaa cccatttgga
     1621 atgcaggcgc gtatgttatg ctcggctgag cggctgcggg accattcttt gcgcgtgtcg
     1681 agctgtgtgc aggccttgcc agacaagccc gcgaaaactg aactgagtga gaaccggacc
     1741 gcgagcggcg tttgagatga ctgtgacctt gaagctttat gcatcgctgg gagcgttctt
     1801 gcccgaaggg gcagagaaga acgtggcgca ggtgccgctc caggatggcg ataccatcga
     1861 gacgctgatc aatcgctaca acgtaccgcc ggaagcctgt catcttgtgc tgttgaacgg
     1921 catcttccag ccgccaggcc ggcgcggcga ggcgcggtta aaggcgggcg atgccgttgc
     1981 ggtttggccg ccggttgcag gcggctgatt gtggccagcc atagccccga tggaccggcc
     2041 gtcgaaaaaa tcatgtcgat gacagcgcgt gaatttgcgg acggctgcga gcggctggcc
     2101 gacgccggtg acgaagtctc gttcagtctt gcaacttttg cagtttcaaa cgggcaggtc
     2161 gccgttcgct tcgaaccgct tgacggcgtc acgctcggcg gccttttgaa actgccgcgg
     2221 gcgcgggtga cgctgacatt ctcaggtctt tccgaccaag agcggaccga ctttctgcgc
     2281 cgtttcgaca tggcgtttca gcgtggcggc gggtaaaggt gcgtacttgg acgcccatgg
     2341 ctaaacgtta ttggccgcct gtccggtgat cagcaaggcg aaagcatatt cgatcgccac
     2401 ttccttcaat tgcgaaaagc ggccggacgc tccgccgtgg ccggcctcca tgttggtgtt
     2461 gagcagaatg taattgccgc tggtgttctt ttcgcgcagc cgggcgaccc actttgcagg
     2521 ctcccagtaa gtgacacgcg ggtccgtcaa gccggtcatg gcgagaatgt gcgggtaggc
     2581 tttgcgctcg acattgtcat aggggctgta ggagcggata cgctcgaatt cttcagcgct
     2641 cttgataggg ttgccccact caagccattc gggcggcgtt aacggcaagg tgtcgtcgag
     2701 catggtgttc aacacgtcga caaacggcac gtcggcgatg atgccgagaa agaggtcagg
     2761 tgccatgttg gcgaccgcgc ccatcaacat tccgccggcc gagccgccat gggcgacgat
     2821 gtgttttcgc ctcgtgtagc cttcggcgac gaggtgctcg ccggccgcaa tgaaatcggt
     2881 gaaggtgttg gttttccttg agagcttgcc gtcggtgtac cagcggtacc ccttgtcctt
     2941 gccaccgcgg atgtgggcaa tggcatagat aaagccacga ttgaccaagg acagccggtt
     3001 tgcaacgaac gatgccggga tcgaaatgcc ataggcgccg tagccgtaga gtaggagcgg
     3061 ggctgagccg tcgaggggtg tatccttgtg gtaaagcagg gaaacgggca ccgtttcgcc
     3121 gtcttcggcg ggtgcataaa ggcggcgggt gacatagtcg gacgggttat ggccgcttgg
     3181 cacttcgcgg cgcttgcgca ggacgcgatc gcgcgtgcgc atgtcgtaat cgtaagtctc
     3241 ggccggtgtc gtcatcgagg aataggtgaa gcgcagggtg gcggtgtcga actcatagcc
     3301 agactgcatg ccaagggcgt aggcctcttc gtcaaatgcg atggcgtgct catcgccgct
     3361 tgcagcgttc cttatgacga ggcgtggcag tgagttttcg cgctccaggc ggacgtcgaa
     3421 atctctgtaa atttccattt caaggatgag acggccgggt ttgtgcggct gaagcgtcgt
     3481 ccagttcttt tcgctcgggt cgcagacggg tgctgtgcaa atccggaaat cttctgcgcc
     3541 atcgctgttg gtcaatatga ttagatcgtc gccgcgatgg catacgtcat actggtgatt
     3601 atgacgcctt gctgcaatca ggcgcggcgg tgaggctggg gcgtcggcat cgagcaggcg
     3661 gatttcgctg gtttgatggt catgagcgtc tatcaaaatg aacttttgag acaatgcctt
     3721 ttcgatgccg acatagaagc catgatcctt ttcctcataa acgagggcgt cgtcgctgac
     3781 gggagttcca agggtgtggc gataaacata aagcgggcgg tggttgccgt cgacgcggac
     3841 gtaaaatagc gtgcgattgt cgttggccca ttgcagggag ccgcgggtgt cggtgatgat
     3901 atcggggagg tcttcgcctg tttccgtgtc gcgaatgcgg atggtgtaaa gttccgagcc
     3961 cttgtcgtcg aaggaatagg cgaggtagcg atgattgggg ctgtgggcgc tggcgccgag
     4021 gtcccaatac gattttcctt cggcctcctt attgccatca agcagaacgg ttgtttcatc
     4081 gccgtcctgc ggcaggcgga gcaggcgcgg gtattggccg ccggtgataa agtcgtacat
     4141 gtaggcaaat gggccgtccg ggtcggggac agacttgtcg tcctctttga tgcgggcctt
     4201 catctcttcg aagagttcag cctgcaattt ctgcgtcggc gccaggacgg cagcggtgta
     4261 ggcgttctca gcctcgaggt gagtgcggat atcggatggc agttttgagg tgtcgcgcat
     4321 gacctcctgc cagttgccgg cgcggagcca ggtgtaggga tcttcgagct tgtggctgtg
     4381 atatgtgcgc tccgtcggat gctggatggc ggccggaggg tgctggctcg cgcggtcgga
     4441 caggtcggtc atttgggctc caagaact
//
LOCUS       DBNHVU010000083         6093 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_478989, whole genome shotgun sequence.
ACCESSION   DBNHVU010000083 DBNHVU010000000
VERSION     DBNHVU010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_478989"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1528
                     /locus_tag="AC16Y7_03120"
     CDS             <1..1528
                     /locus_tag="AC16Y7_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008188575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HIR5708403.1"
                     /translation="CSYHLQEAGATPAQELAFALATAIAVLDAVKASGEVPEEKFPRV
                     VGAISFFVNAGVRFITEVSKMRAFVDLWDEIARERYGVEEPKFRRFRYGVQVNSLGLT
                     EPQPENNVYRILLSMLAVTLSKKARARAVQLPAWNEALGLPRPWDQQWSLRLQQIVAY
                     ETDLLEYADIFDGSEVIDRKVEELKEQACAELAKLDAMGGAVASIDYMKESLIASNAE
                     RIADIERGDQTLVGVNAYTETEPSPLDSGVEGILTVPESVEIEQIAALQAWRQKRDDA
                     SVRETLRELKDAASEGRNIMPPSIAAAKAGVTTGEWGAVLREVFGEYRAPTGIGASHE
                     IDEARLQDVRELVSQVSGKLGRTLTFVVGKPGLDGHSNGAEQIAVRADDVGMSVVYDG
                     IRLTPDQIVEQAKEAKAHVVGLSILSGSHVSLVRDVVQKLREAGMGEVPVVVGGIIPA
                     EDVNVLKQCGAAAIYTPKDFQLNEIMTGIVDLVDQKVEVLAEADVPVPPLEAKEKLHL
                     "
     gene            1644..1820
                     /locus_tag="AC16Y7_03125"
     CDS             1644..1820
                     /locus_tag="AC16Y7_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708404.1"
                     /translation="MDFKLSTPRRQTFLISVILAALVVIGMIVPLPIFTSYGLGLLLI
                     AYLILLAGVLMDNA"
     gene            complement(1842..2102)
                     /locus_tag="AC16Y7_03130"
     CDS             complement(1842..2102)
                     /locus_tag="AC16Y7_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5708405.1"
                     /translation="MCLAARPYDLVLMDTVLPDYDGSQMAKRIRTMQAPSSDVPIVGI
                     TASGSSEDLQGLVEAGLNARVTKPIQGRALYAALVPFLPAQA"
     gene            complement(2102..2932)
                     /locus_tag="AC16Y7_03135"
     CDS             complement(2102..2932)
                     /locus_tag="AC16Y7_03135"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5708406.1"
                     /translation="MESAAAMANQLLSSPAGVSQRHDIETFAQSARALFDTLHDVLDY
                     ADLETGAAEVALEPFDLHALVKDAASTLQARAGAKSLTSAVEMTPDCPRFIVGDVRRV
                     RQVLMGLIEAALQRTTEGSIRLYASVNEAAHPFTVRFDITDTGAGLTEAEIDNLFQPC
                     SGTSRIGGSLGLPIARRLAAAMGGGLGCTSAVGQGTLHWFTFQSVAADEQDAAKAEAA
                     PKPETPAPQSETKAPREGALSGHVLLVEGNSVNRMLIGAYLDEIGLTYTRRPTTVRRR
                     "
     gene            3410..3661
                     /locus_tag="AC16Y7_03140"
     CDS             3410..3661
                     /locus_tag="AC16Y7_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR5708407.1"
                     /translation="MIVCSCSMISAQDIHDAVSALRTKDPFVVLTPGLIYKTLGKQPS
                     CGSCLPLITKLMLSYDDEGPSSAASLPHRSPFDNSPTAN"
     gene            3715..4209
                     /gene="bfr"
                     /locus_tag="AC16Y7_03145"
     CDS             3715..4209
                     /gene="bfr"
                     /locus_tag="AC16Y7_03145"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HIR5708408.1"
                     /translation="MKGNPTVIEYLNKALTSELTAVNQYWLHYRLLDDWGYTKLAQKE
                     REESIEEMHHADKLVTRIIFLEGFPNMQKLDPLMIGQNIKEILECDLKAEYGARALYM
                     EARAACRKEEDYASMDLFEELLRDEEGHIDFIETQLGLLSDIGPQMYGQLNADSAKDV
                     ADSD"
     gene            4233..4907
                     /locus_tag="AC16Y7_03150"
     CDS             4233..4907
                     /locus_tag="AC16Y7_03150"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5708409.1"
                     /translation="MTLPGLDGPRLPPAAGGSAQQLVVFLHGYGADGNDLIGLGREWA
                     SLLPHATFVSPNAPEPCAMSPMGRQWFDLSMGDMSIIAEGVKSAAPALNAFLDAELAR
                     HDLPASALALVGFSQGTMMALAVGLARNAPPKAIVGYSGALATLEALPAPGQGPAILL
                     VHGDRDEVIPVDAMHMAREALSQAGHPVEWHVSQGIGHGIDGEGLRLGGNFLRQAFAD
                     AGATTP"
     gene            5035..5601
                     /locus_tag="AC16Y7_03155"
     CDS             5035..5601
                     /locus_tag="AC16Y7_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR5708410.1"
                     /translation="MNVATASAAPGTYPALVLNADYRPLSYYPLSLWGWQDAVKAVFL
                     DRVNIVSEYEHAVHSPSFEMKLPSVVSLKSYVRPALYPAFTRFNVFLRDRFTCQYCGD
                     RQELTFDHLIPRSRGGLTRWENVVTACAPCNLAKGGEMPTRAKMWPAQKPYRPTVFEL
                     HRNGRLFPPNYLHESWLDYLYWDTELEP"
     gene            complement(5615..>6093)
                     /locus_tag="AC16Y7_03160"
     CDS             complement(5615..>6093)
                     /locus_tag="AC16Y7_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HIR5708411.1"
                     /translation="TVGVVLLVPSLQAQVAVAAGPVGNWVDDRFGGFNATSLWGQFGL
                     GLLLGAVWVPCVGPTLGAASILAAKGEHLGQVALTMLAFGVGAALPLMVLGFASREAM
                     QRWRGRLAEAGKGGKMLLGALLVAVGLLVATGADKKLEAFLVEASPQWLTNLTTRY"
BASE COUNT         1144 a         2000 c         1870 g         1079 t
ORIGIN      
        1 ctgctcctat catttgcagg aggcaggcgc gacgccggca caggaactcg ccttcgcgct
       61 ggccacggca atcgccgtcc tcgatgcggt caaggcctcc ggcgaagtgc cggaagagaa
      121 gtttccgcgc gtggtcggcg ccatcagctt cttcgtcaat gcaggcgttc gcttcattac
      181 cgaggtgagc aagatgcgcg ccttcgtcga tctctgggat gagatcgcgc gcgaacgcta
      241 cggcgtcgag gagccgaaat tccggcgctt ccgctatggc gtccaggtga actcgcttgg
      301 cctcaccgag ccgcagcccg agaataatgt ctaccgcatc ctgctatcga tgctggcggt
      361 gacgctgtcg aagaaggccc gcgcccgcgc cgtgcagttg cctgcgtgga acgaggcact
      421 cggcctgccg cgcccctggg atcagcaatg gtcgctgcgg ctgcagcaga tcgtcgccta
      481 cgagaccgac ctcctggaat acgccgacat cttcgatggc tccgaagtga tcgaccgcaa
      541 ggtcgaggag ttgaaggaac aggcttgcgc cgaactcgcc aaactcgatg ccatgggcgg
      601 cgccgtggcg tccatcgatt acatgaaaga gtcactcatc gcttcgaacg ccgagcgcat
      661 tgccgacatt gagcgcggcg atcaaacact ggtcggcgtc aacgcctata ccgagaccga
      721 accgtcgccg ctcgattctg gcgtcgaagg cattctcacc gtgccggagt ccgtggagat
      781 cgagcagatc gccgcgctgc aagcttggcg gcagaagcgc gatgacgcga gcgtgcgcga
      841 aactttgcgc gagctgaagg atgcggccag cgaaggccgc aacatcatgc ccccgtcgat
      901 cgccgccgct aaggccggcg ttaccaccgg cgaatggggc gcggtgctgc gcgaagtctt
      961 cggtgaatat cgcgcgccca ccggcatcgg cgcctcgcac gagatcgacg aggcgcgcct
     1021 gcaagacgta cgcgagctgg tgagccaagt ctcgggcaag ctcggccgca cgctcacctt
     1081 cgtcgtcggc aagcccggcc tcgatggcca ttccaatggt gccgagcaga tcgccgtgcg
     1141 cgcggacgat gtcggcatga gtgtcgtcta tgacggcatc cgcctgaccc ccgatcaaat
     1201 cgtggagcaa gccaaagagg ccaaggccca tgtcgtggga ctttccatcc tctccggctc
     1261 ccacgtgtcc ctggtgcgcg acgttgtgca gaagctgcgc gaggcgggta tgggcgaggt
     1321 gccggtcgtt gtcggcggca tcattccggc cgaagacgtc aacgtcctca agcagtgcgg
     1381 cgccgcagcc atctatacgc cgaaggattt ccagctcaac gagatcatga ccgggatcgt
     1441 cgatctggtg gaccaaaagg tcgaggtgct ggccgaggcg gacgttcccg tgccgccgct
     1501 ggaagccaag gaaaagctgc acctttagaa attccccggt tcaggggcca tgacagcgga
     1561 gcaactttgc cgctagagtc ctggcaacgc tgatttttct tctggcgtca tgccaaaaga
     1621 ggcgcatcaa ggggggggac atcatggact tcaaactgag cacgccgcgc cggcagacct
     1681 ttctcatctc cgtcatcctg gccgcactcg tggtgatcgg catgatcgta ccgctgccga
     1741 tcttcacctc gtacggtctc ggcttgctgc tgatcgctta cctgatcctg ctcgccggcg
     1801 tcctgatgga caatgcctga cgcgcgctgg cctgacgcga gctaggcttg cgccggcagg
     1861 aacggcacaa gcgcagcgta gagggcgcgg ccctggatcg gcttcgtcac gcgcgcattc
     1921 aggcccgcct cgacgaggcc ctgcagatcc tcagaggacc cagatgcggt gatgccgacg
     1981 atcggtacgt ccgacgacgg cgcctgcatg gtgcggatgc gctttgccat ctgtgagccg
     2041 tcatagtccg gcagcaccgt gtccatgaga acgagatcgt agggccgagc cgcgaggcac
     2101 atcagcgccg ccgcaccgtt gtcggccgtc tcgtataggt gagaccgatc tcgtcaagat
     2161 aggcaccgat cagcatgcga ttgacgctgt tgccctcgac cagcagcacg tgacccgaca
     2221 gcgctccctc gcggggggct tttgtctcgg actgcggtgc cggcgtttcc ggtttcggcg
     2281 cggcttcagc ctttgccgcg tcttgctcgt cggcggcaac agactggaag gtgaaccaat
     2341 gaagcgtgcc ctgcccgacc gcgctggtgc agccaagccc gccgcccatc gccgcggcta
     2401 ggcgccgcgc gattggcagc ccaagagagc cgcctatgcg cgatgtgccc gagcacggct
     2461 gaaacaagtt atcgatttcc gcttcggtga ggccggcgcc cgtatcggtg atgtcgaacc
     2521 gcaccgtgaa gggatgggcc gcctcgttga cgctggcata gagtcgaatc gagccttcgg
     2581 tcgtccgttg cagcgcggcc tcgatcaggc ccatgagcac ttggcgcacc cggcgcacgt
     2641 cgccaacgat gaagcgtgga cagtctgggg tcatctccac cgcgctggtc aggctcttgg
     2701 cgcccgcgcg cgcttgcaaa gtcgaagctg catccttcac cagcgcatgc aggtcgaacg
     2761 gctcgagggc cacttccgcc gcgccggtct ccagatctgc gtagtccaga acgtcgtgaa
     2821 gcgtatcgaa cagcgcccgc gccgactgcg cgaaggtctc aatatcgtgc cgctgggaaa
     2881 cgccagccgg gctggacaga agctggttgg ccatggcggc cgccgattcc atgggcgcgc
     2941 gcgcaggtcc ctgcccatca gcgcgagcag cccggtcttg gccgcatcgg cggcttctgc
     3001 ggcctcgcgg gccttacgct ccgcgtcgag cccgctcatg agcccttcga tcttcacatt
     3061 ggtattcgcc ggctgagccc ggttgatttg catgatacgc ccccattgct aggccttgtt
     3121 cgaccccgtt gttgaggcgc atgttctcag acccggctta aatccgagct aaactgcgtg
     3181 gtttatgagg tcctaaggtg ggaatgccct cgtccgggcg gatgactggc ggagcccggc
     3241 aggcccgcct gggaaagccg gcaaatagcc tgcttcctca ccggggccaa cgtcccttgc
     3301 cgccaaaggg tttctacttt agaattatcc taatctaaaa ctggccgaat tggtcagcgg
     3361 cggccgtgcg atgtggagac aatccgcccg cgctgtcata gagaaaagca tgatcgtctg
     3421 ctcctgttcc atgatttcag cccaggacat tcacgatgcg gtctcggccc tacgtaccaa
     3481 ggaccccttc gtcgtgctca cgccggggct catctacaag acgctcggca agcagccgtc
     3541 ctgcggcagt tgcctgccac tcatcaccaa gctcatgctc agctacgacg atgaggggcc
     3601 gagctccgcg gcctcgcttc cccatcggtc accattcgat aacagcccca cggcgaacta
     3661 ggcggacacc acagaacacg tcacaatgaa cagcaaggtt gagaggagat cacgatgaag
     3721 ggcaatccaa cagttatcga gtacctgaac aaggcgctca ccagcgaact caccgcggtc
     3781 aatcagtatt ggctgcacta ccgtctgctc gatgactggg gctacaccaa gctcgcccag
     3841 aaggagcgcg aggagtccat cgaagagatg catcacgccg acaagctggt gacgcgcatc
     3901 atcttcctcg agggcttccc caacatgcag aagctcgatc ccttgatgat cggccagaac
     3961 atcaaggaaa tcctcgaatg cgatctcaag gccgagtacg gcgcccgcgc gctctacatg
     4021 gaagcgcgtg ccgcgtgccg aaaggaagag gattatgcga gcatggatct gttcgaagaa
     4081 ctgctgcgcg atgaagaagg gcatatcgac ttcatcgaga cgcaactcgg tcttctcagc
     4141 gacatcggtc cgcaaatgta tggccagctc aacgccgact cggccaagga cgtcgccgac
     4201 agcgactagg tgcggggagc gggttcacac acatgacctt gccgggactt gatgggccgc
     4261 gcctgccgcc ggccgcgggc ggcagcgcac agcagctcgt ggtgttcctc cacggctatg
     4321 gcgccgacgg caacgacctg atcggcttgg ggcgggagtg ggcaagcctc ctgccgcacg
     4381 ccacgttcgt ctcgcccaat gcgccggagc cgtgcgccat gtcgccaatg ggccggcagt
     4441 ggttcgacct ctccatgggc gacatgtcga tcatcgccga gggcgtgaag agcgccgcac
     4501 ccgcgctgaa tgcctttctc gacgccgagc tcgccaggca cgacctgccg gctagcgccc
     4561 tcgcccttgt cgggttcagc cagggcacca tgatggcctt ggctgtcggc cttgcccgca
     4621 acgccccgcc caaggccatc gtcggctatt caggcgcgtt ggcgacgctc gaggccttac
     4681 cggcgccggg ccaaggcccc gccatcctcc tggtgcacgg cgacagggac gaagtgattc
     4741 cggtcgatgc catgcacatg gcccgcgaag cccttagcca agccggccac cccgtggagt
     4801 ggcatgtttc acaaggcatc ggacacggca ttgacgggga gggattgcgc ctcggcggca
     4861 acttcttgcg tcaggccttc gctgatgcgg gcgcaaccac gccatagggg ctcgtgaatg
     4921 gtaaaccaca gcatgtgggg cccttcacat tcatgacacg tccttatagt atctcgcgcg
     4981 acagacgagc tgggaacccc gagtgcgacg ggcagtaacg aggacctttt ggccttgaac
     5041 gtcgcaacag catcggccgc tcccgggacc tatcccgcac tcgtgctgaa tgccgattat
     5101 cggcccctct cttactaccc tctatcgcta tggggttggc aggacgcggt gaaggccgtg
     5161 ttcctcgacc gcgtcaacat cgtctccgag tacgagcacg ccgtacactc gccgtcgttc
     5221 gagatgaagc ttcccagtgt cgtctcgctg aagagttacg tgcgcccggc gctctatccc
     5281 gccttcaccc ggtttaacgt gttcctgcgc gaccgcttca cctgccaata ttgcggcgac
     5341 aggcaggagc tcaccttcga tcatctgatc ccccgctccc ggggcgggct cacaaggtgg
     5401 gagaacgtgg tcacggcctg cgcgccgtgc aatctcgcca aaggcggtga gatgccgacc
     5461 cgcgccaaga tgtggccggc gcagaagccc taccggccga cggtgttcga attgcaccgc
     5521 aacgggcggc tgttcccgcc gaactatctg cacgaaagct ggctcgacta tctttactgg
     5581 gataccgagc tggagccgta ggccgagtgt cttattagta gcgcgtcgtc aggttcgtca
     5641 gccattgcgg cgaagcctcg accaagaacg cttccaattt cttgtcggcg ccggtcgcca
     5701 ccagcagtcc caccgcgacg agaagtgcgc caagcagcat cttgccgcct ttgccagctt
     5761 ctgcgagacg cccacgccag cgctgcatgg cctcgcgcga ggcaaagccc aacaccatga
     5821 gcggcaacgc cgcgccaact ccgaacgcaa gcatggtgag cgccacctgc ccgagatgct
     5881 cacccttggc agcaaggatt gacgccgcac caagcgttgg ccccacgcac ggcacccaca
     5941 ccgcaccaag cagcagcccc agtccgaact gaccccacag gctcgtggcg ttgaacccgc
     6001 cgaaacggtc gtcgacccaa ttgccgacgg gaccggccgc cacggcgact tgcgcttgta
     6061 ggctcggcac gagcagcacg accccgacag tga
//
LOCUS       DBNHVU010000084         3559 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1742791, whole genome shotgun sequence.
ACCESSION   DBNHVU010000084 DBNHVU010000000
VERSION     DBNHVU010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1742791"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..288)
                     /locus_tag="AC16Y7_03165"
     CDS             complement(100..288)
                     /locus_tag="AC16Y7_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014332804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HIR5708412.1"
                     /translation="MDQFNISMRKFLKEVGVTSQQAIEDAVREHNLAGKGKLKVKMLL
                     TSETADLNHVVEGEIELP"
     gene            503..955
                     /locus_tag="AC16Y7_03170"
     CDS             503..955
                     /locus_tag="AC16Y7_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013487.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5708413.1"
                     /translation="MTFRLSQLIVLFLLPFAPLALAHEGATGVVKERMDLMDTQKEAM
                     KVIGAMAKGKVPFDARAAAKAAHEIEVTAKKIPELFPEGTGGRPSEAKPEVWTQWDKF
                     TKDAEALGVAANDLVAALEASTPDWKAKFKGVIDACKTCHKTFREEDD"
     gene            1040..1918
                     /locus_tag="AC16Y7_03175"
     CDS             1040..1918
                     /locus_tag="AC16Y7_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR5708414.1"
                     /translation="MIDKLEFIIALARERHFGHAAEDCGVSQPTFSAGIKQLEDTLGV
                     LLVERGSRFRGFTPEGERVLDWARRIVSDTRAMRQEVEALKHGLTGHIRIAAIPTALP
                     IVGLITTPYRAQHPDVSFTILSRNSIEVLGMLENLEIDAGVTYLDNEPLGRVQTVPLY
                     SEEYRLLASSDSPLGDRETVTWAEVGKVPLCLLTPDMQNRRIIEQLLRHEGADVAPTL
                     ESDSIVVLFAHVRTGKWASVMPAKLAEVLGLTEKVRAIPIVNPEVTHSIGLVVPQRNP
                     LTPLINALVSEAKQLF"
     gene            2010..2705
                     /gene="modB"
                     /locus_tag="AC16Y7_03180"
     CDS             2010..2705
                     /gene="modB"
                     /locus_tag="AC16Y7_03180"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HIR5708415.1"
                     /translation="MLDLTPEELTAITLSLRVAAVATLVSLPFGIAVAMLIGRGRFWG
                     RPLVNALVLLPLVLPPVVTGYVLLLLFGRQGPIGSWLDETFGIVLAFRWTGAALACAI
                     MGFPLLVRAIQLSVEAVDPRLEQAASTLGANRAYVFLTVTLPLIAPGIIAGAILAFAR
                     SMGEFGATITFVSNIPGETQTLPSLIYTFTQVPGGDVGALRLTLVSAAISLGAILAAE
                     ALAQFIGRRVRAR"
     gene            complement(2726..>3559)
                     /locus_tag="AC16Y7_03185"
     CDS             complement(2726..>3559)
                     /locus_tag="AC16Y7_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated
                     beta-propeller protein"
                     /protein_id="HIR5708416.1"
                     /translation="GIVLSPDFKELFVAAGDGDIIDVIDTKTMEVVRTLESGPDPELM
                     AIDSKGEVIYIANEDDSLVTVMDIKSGDVLGEVPVGVEPEGMIVSPDDRYTAATSEST
                     SMAHIIDNKSIKLVGNILVDSRPREAKFTKNGKELWVSSEIGGTISVIDTSDWDNPNN
                     WKPVEKIGFEVAGLRPEQLQPVGMDFNEDDSKLFVPLGPANRVAVIDAKTKEVLNYIL
                     VGQRPWHGEFSPDYKKYYVANGLTNDLTVIDVDTLKAEKSVPVGRLPWGVAIMPGKAG
                     E"
BASE COUNT          636 a         1122 c         1074 g          727 t
ORIGIN      
        1 taaaggagat gaagtggact tcatcccctt ctccgctttt gggtttggcg accgcctgaa
       61 cctgaccagc gaagtctcat aagggacgtc acgccagtcc tagggcaact cgatctcgcc
      121 ttcgaccaca tggttgaggt ctgcggtctc gcttgtgaga agcatcttca ctttgagctt
      181 cccctttccg gcaaggttat gctcccgcac agcatcctct atcgcttgtt gggacgtgac
      241 ccctacctct ttaagaaact tgcgcatcga aatgttaaat tggtccatct ctgcaatcct
      301 cctgagagtc ttcattctaa attacccgat ccgtttcacc atctccatga cctggacggg
      361 ctctcgactg ggcatcggta tgggtatggg catggtggct cccaggaagg agcccagcac
      421 agtccggcta ctatgataca agcatctcag ggcgcatagc cgagtgtcgg cctccaatag
      481 gattagctgg gtgagtgacc aaatgacctt caggctatca caactgatag ttctattcct
      541 tctgcctttc gcgccgctgg ctcttgcgca tgaaggcgcc accggcgttg tcaaagagcg
      601 catggacctg atggacacac agaaggaggc catgaaggtc atcggcgcga tggccaaggg
      661 aaaggtcccg ttcgacgcca gggctgcggc gaaggccgcg cacgagatcg aggtgaccgc
      721 caagaagatc cccgagctct tccctgaagg caccggcgga cgtcccagcg aggccaaacc
      781 ggaggtctgg acccagtggg acaagttcac gaaggacgca gaggcgcttg gcgtcgccgc
      841 aaacgacctt gtcgcggcac tggaggcgag cacaccggac tggaaggcga agttcaaggg
      901 cgtgatcgac gcctgcaaaa cctgccataa gacgttccgg gaagaggacg actagggaac
      961 acagcttttc gcgcgttgtt gaggctgcac gccggggcca cattccctat agtccccgcg
     1021 gagagacacc cggcacggta tgatcgacaa gctcgaattc atcatcgctc tggcgcgcga
     1081 gcggcatttc ggccacgcgg cggaagactg tggcgtcagc cagccgacct tttccgccgg
     1141 catcaagcag ctggaggaca cgcttggcgt gctcctggtc gagcgcggct cgcgcttccg
     1201 cggctttacg cccgaggggg agcgggtgct ggactgggcg cggcgcatcg tgagcgatac
     1261 ccgcgccatg cgccaggagg tcgaggcgct gaagcatggc ctgaccggtc atatccgcat
     1321 cgccgcgatc cccacggccc tgccgatcgt gggcctgatc accacgccct accgggcgca
     1381 gcacccggat gtgagcttca ccatcctgtc gcggaactcg atcgaggttc tcggcatgct
     1441 ggaaaacctc gagatcgatg cgggcgtgac atatctggac aatgagcctt tgggccgcgt
     1501 ccagaccgtg ccgctctata gcgaggagta ccgcctgctg gcctcctccg acagcccgct
     1561 cggcgaccgg gagaccgtga cttgggccga ggtgggcaag gtgccgctct gcctgctcac
     1621 gcccgatatg cagaatcgcc ggatcattga gcaattgctc cgccacgagg gggccgacgt
     1681 ggccccgaca ctcgaatcag attcgatcgt cgtgttgttc gctcacgtgc ggaccggcaa
     1741 gtgggccagc gtcatgcccg caaagctcgc cgaggtcttg ggcctcacgg agaaggtccg
     1801 ggctattccc atcgtgaatc cggaagtcac ccactcgatc gggctcgtgg tgccccagcg
     1861 caacccactg acacctttga ttaatgccct cgtcagcgaa gccaagcagc tcttctaggt
     1921 cgcggtttca gggcacgtag gccccctatc ctcgccgctt gtggcgcctg cgtcttaagc
     1981 ctcatcgcgg caggtggtaa tactgttccg tgcttgacct gacgcccgag gaactgaccg
     2041 ccatcacgct gtctttacgc gtggccgccg tggccacgct ggtgagcctg ccgttcggga
     2101 tcgccgtggc catgctgatc ggccgcggac gcttctgggg acgcccactt gtcaatgcgc
     2161 ttgtactctt gccgctggtg ttgccgccgg tggtgacggg ctatgtgctg ctgcttctgt
     2221 tcggacggca gggcccgatc gggtcttggc tcgacgagac gttcgggatc gttctcgcct
     2281 tccgctggac gggcgccgcg ctggcttgcg ccattatggg gtttccgctt ctggtgcggg
     2341 caatccaact atccgttgag gcggtcgatc cgcggctcga acaagctgcg agcacgctcg
     2401 gcgcgaaccg cgcctatgtg ttcctcacgg tgacgctgcc tttgattgcg cccgggatca
     2461 tcgccggcgc cattctcgcc ttcgcccgct ccatgggcga gttcggcgcg acgatcacct
     2521 tcgtgtcgaa catccccgga gaaacgcaga cgctgccgag cctgatctac acgtttacgc
     2581 aagtaccagg cggcgacgtt ggcgcattgc gactcacgct ggtctcagcg gcgatctcgc
     2641 ttggcgcaat cctggcggcg gaggcgctcg cccagttcat cgggcggcgc gtccgcgcca
     2701 gatagaccgc cgccttcggg aaactctatt cgcccgcctt gcccggcatg atggcgacgc
     2761 cccagggcag ccgccccacg ggcaccgact tctcggcttt caacgtatcg acgtcgatca
     2821 ccgtaaggtc attggtgagg ccgttggcaa catagtactt cttgtagtcg gggctaaact
     2881 cgccatgcca cggccgctga ccgaccaaga tgtagttgag cacctctttc gtcttagcgt
     2941 cgatcaccgc aacccgattg gcgggaccca agggcacgaa gagcttcgaa tcgtcctcgt
     3001 tgaagtccat gcccacgggc tgaagctgct cgggccgcag gccggcaact tcgaagccga
     3061 tcttttccac cggcttccaa ttgttgggat tgtcccaatc gctggtatcg atgacggaaa
     3121 tcgtgccgcc gatctcggag gacacccaaa gctccttgcc gtttttggtg aatttggctt
     3181 cgcgcggccg gctgtcgacg agaatattgc ccacgagctt gatcgacttg ttgtcgatga
     3241 tatgcgccat gctggtgctc tcggacgtgg cagctgtgta gcggtcgtca gggctcacga
     3301 tcatgccttc gggctccacg cccaccggca cttcgccgag cacgtcgccg ctcttgatat
     3361 ccatgacggt gacgaggctg tcgtcctcgt tggcgatgta gatcacctcg cccttggaat
     3421 cgatggccat aagctctgga tcaggacccg attccagtgt ccgtacgacc tccatcgtct
     3481 tggtgtcgat gacgtcgata atgtcaccgt cgcccgccgc cacgaacagc tccttgaagt
     3541 cggggctgag cacgatgcc
//
LOCUS       DBNHVU010000085         3527 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_257891, whole genome shotgun sequence.
ACCESSION   DBNHVU010000085 DBNHVU010000000
VERSION     DBNHVU010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_257891"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="AC16Y7_03190"
     CDS             <1..1191
                     /locus_tag="AC16Y7_03190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR5708417.1"
                     /translation="FQIAEALRKVITRHYPEIKIEVFETRGSLHNAKLLDEGAVQLAT
                     LQADQATGSSARLLAELYPDTFQIVVRGDSGIRSISDLIGKRIALPPEQSGEYEAFWF
                     LASYYSLTNESLRAFTGTERTTDWLLMNGDVDALFRIRAPGDRSILRLIQKVNGRIIP
                     IPQAAAIQLRHPALEAGSIPVGSYKGRPAIPGSDEQTISVKQLLLAREQVPPDVVAKV
                     ASVLFEHRRELMEVVPIAGQITTPDASGQTLVPIHAGVRAFWDRDKPSFIQENAEPIA
                     LLITIAVVTASAYLQLANRRRKKVMDAYNRELIALGRRARNAPDFGTLDECNNALAEF
                     VGRIVHETERGRINTAEFTLFNFAYDAVEDAVKDRSAQLERQERKEIGTAFARMTTKQ
                     ATED"
     gene            1198..2304
                     /locus_tag="AC16Y7_03195"
     CDS             1198..2304
                     /locus_tag="AC16Y7_03195"
                     /inference="COORDINATES: protein motif:HMM:NF014763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HIR5708418.1"
                     /translation="MARRLISIVLLSVLGLAVGLATAALVERHQGNEALSVTFDRLHL
                     FHDLRQAALEDYLKSMASDVRAASENPRVLEAMEKLNFAWKTYGPDARKVLPRLYTDE
                     NPALPKERAQLLEAGDGSYYSQYHRAFHGWARKFLEHFGYYDLFLINPRGDIIYSVAK
                     ERDFATSLKTGPYRKSPLGEVFHRAIVNPSASVDFSDFARYAPSGDDPAAFAAHAITK
                     GGQVIGVFAVQIPAEPLNELMQFTAGMGKTGETYLVGPDGLMRSQSRFIEKPTLLEVE
                     VDNLSIHEGRAGKSGARIVEDYRGIDVLSVYAPVNFGGQPWILLAEIDKEEAVGTERS
                     WIAIAAGILAGLLAMGLAYLLWHVLRSAPEAASP"
     gene            2301..2429
                     /locus_tag="AC16Y7_03200"
     CDS             2301..2429
                     /locus_tag="AC16Y7_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708419.1"
                     /translation="MSIHLHHIELGGTVDDEAALVQFQKGWSIYQKVVDHNVLAHR"
     gene            2457..3032
                     /locus_tag="AC16Y7_03205"
     CDS             2457..3032
                     /locus_tag="AC16Y7_03205"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5708420.1"
                     /translation="MSVPEPIDLLDIACGDAGQMRGALADTKVRHYRGIDLSQPALEL
                     AARNLDGVPFDVDLDHSDFVTELAGRHHTSDVSWCGLSIHHLTTDGKRDLLQALCETT
                     RRFLMIYEPILLEGEGFEGYLDRYARLNRSAWTFLAPEEWDQLFHHISTCDLPESPST
                     WLTLGREAGFAEARQVYLDPPGLYAVYRYDR"
     gene            complement(3066..>3527)
                     /locus_tag="AC16Y7_03210"
     CDS             complement(3066..>3527)
                     /locus_tag="AC16Y7_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR5708421.1"
                     /translation="TQYAKVPTGGGPMPSDDEIAIRAAVSQWFDVLNAMLNGDAEPLA
                     ALYSHNDDVTYMGAEGTYRVGWSATFADWKAQAKKDIGGSAEGRDIHVVVSGDLATAS
                     HLTVSSIANAQGERTEVTVRETSVFRKEDGAWRMIAHHADAIQDLVEAFGN"
BASE COUNT          677 a         1093 c         1051 g          706 t
ORIGIN      
        1 tttcagatcg ccgaggcgct tcgcaaggtc attacccggc actatccgga gatcaagatt
       61 gaggtcttcg agactcgcgg ctcgctgcac aacgctaagc tgctcgatga aggcgcggta
      121 cagctcgcga ctctccaggc tgatcaagcg actggaagct cagcccgcct actggcggaa
      181 ctctatcccg atacgtttca gatcgtggtg cgcggcgaca gcggtatccg ctcgatctcc
      241 gatctaatcg gcaagcgcat cgcgcttccg cccgagcaaa gcggcgagta cgaagcattt
      301 tggttcctcg ccagctacta ctcgctgacc aatgagagtt tgcgggcgtt caccggcacc
      361 gagcgcacta ccgattggct gctgatgaac ggcgatgtag acgcgctgtt tcgcattcgc
      421 gcgccaggcg atagatcgat cttgcgactc atccagaagg tcaacggacg catcattcct
      481 attcctcaag ccgcggcgat acagctcaga caccctgccc ttgaggccgg cagcattccc
      541 gtaggctcct ataaggggcg ccccgccata cccggaagcg atgagcagac catttcggtc
      601 aagcagctct tgcttgcgcg tgagcaagtg ccgcccgatg tggtcgccaa ggtcgcgtcg
      661 gttcttttcg agcatcgccg cgagttgatg gaggttgtgc caatcgccgg gcagatcacg
      721 acgccggatg caagcggcca gaccttggta ccgatccatg ctggcgtgcg cgccttctgg
      781 gatcgcgaca agccgtcctt catccaggaa aacgccgagc ccatcgcgct cttgatcacg
      841 atcgctgtgg tgacggcctc ggcctacctt cagcttgcca accggcgacg aaagaaagtg
      901 atggatgcct ataaccgcga gctgatcgcc ctaggacgcc gagcgcgcaa cgcaccagac
      961 ttcgggacgc tagacgaatg taacaacgcg ctcgccgagt tcgtggggcg catcgtgcat
     1021 gaaacggagc gggggcggat caacaccgcg gagttcacac tgttcaactt cgcctatgac
     1081 gcggtggaag acgcggtcaa ggatcgcagc gctcagttgg agcgccaaga gcggaaagaa
     1141 attggtactg cgtttgcccg gatgacgacc aaacaagcga ctgaggacta ggcaagcatg
     1201 gcgagacggc tgatttcaat cgttctgctg tcggttctcg gacttgcagt gggacttgcc
     1261 acagctgctt tggtcgaacg gcaccaaggc aacgaggcgc tgagtgtcac attcgatcgc
     1321 ttgcacctgt ttcacgacct caggcaggcg gcgctggagg actatctgaa gtccatggcg
     1381 tctgacgtgc gcgcggccag cgagaacccg cgcgtccttg aggccatgga gaagctcaac
     1441 tttgcctgga agacgtacgg ccccgatgca cgcaaagtat tgccgcggct ctataccgac
     1501 gagaatcccg ccctacccaa agagagggcg cagctcctgg aagcgggcga cggctcgtac
     1561 tacagccaat atcaccgcgc ctttcacggc tgggcacgga aatttctcga acatttcggc
     1621 tattacgatc tcttcctcat caatccgcga ggcgacatta tctacagtgt ggccaaggag
     1681 cgcgacttcg ccaccagcct caagacgggc ccctaccgca agagcccgct gggagaggtg
     1741 tttcaccgcg ccattgtgaa cccgtctgcc tccgtcgact tctccgactt cgcacgctat
     1801 gcgccgagcg gtgacgaccc ggccgcattt gccgcccatg ccatcaccaa aggcggccaa
     1861 gtcatcggtg tgttcgccgt gcagattccg gccgagcccc tcaatgagct catgcaattc
     1921 accgcaggga tggggaagac cggcgagacc tatctggtcg gcccggacgg tctgatgcgc
     1981 agtcagtcac gcttcatcga gaaaccgacg ctgctggagg tggaggtcga caatctttcg
     2041 atccacgagg gcagggccgg caagagcggc gcccgcatcg tcgaagacta ccgcggcatc
     2101 gacgtccttt cggtctacgc gccggtcaat ttcggcggtc agccttggat cttgctggcc
     2161 gagatcgata aggaggaggc cgtcgggact gagcgctcat ggatcgccat tgcggccgga
     2221 atcctcgccg ggctcctagc gatggggctc gcctatctcc tttggcacgt cttgcgcagc
     2281 gcccctgagg ccgcgtcgcc ttgagtattc accttcacca catcgagctc ggcggaacgg
     2341 tcgacgacga ggccgcgctc gtgcagtttc aaaaagggtg gtcgatctat cagaaggtcg
     2401 tggatcacaa cgtccttgcc cataggtagg tcggggacgt tcttcaccgt gccctgatgt
     2461 cggttccaga gccgattgat cttctcgata tcgcctgcgg cgatgcgggg cagatgcgag
     2521 gcgcccttgc ggacaccaag gtccgccact atcgggggat cgatctctcg cagcccgcgc
     2581 ttgagctcgc cgcaaggaat ttggacggcg tccccttcga cgtcgacctc gatcattcag
     2641 attttgtgac ggagttggcc gggcgccatc acacatcgga cgtttcttgg tgcgggctct
     2701 ccattcatca cctgacgacg gatgggaagc gcgacctgct ccaggccctt tgcgaaacca
     2761 cgcgccggtt cctcatgatc tatgagccga tcctgctcga aggcgagggc ttcgagggct
     2821 accttgatcg ctacgcgcgc ctcaacaggt cggcctggac tttccttgcg cctgaagagt
     2881 gggatcaact gtttcaccac atctccacct gcgatctgcc tgagagtccc tcgacctggc
     2941 tgacgctcgg ccgcgaggcc ggttttgcag aagcacggca ggtctatctc gatccgcccg
     3001 gcttgtacgc ggtttaccgt tacgatcgtt gaaccgtcga agtggcggca tcctgaagct
     3061 ggcagctagt ttccgaacgc ctcgaccaaa tcttgaattg catccgcatg gtgcgcgatc
     3121 atccgccagg ccccgtcctc tttgcggaag acgctggtct cccgcacggt gacctcagtt
     3181 ctctcgcctt gggcattggc gatactgctg acggtgaggt gggacgccgt ggccaagtcg
     3241 ccgctgacca cgacgtggat gtcgcggccc tcggcggagc ctccgatatc cttcttggcc
     3301 tgtgccttcc aatcggcgaa cgtagcggac cagccgaccc gataggtccc ttcggcgccc
     3361 atataggtga cgtcatcatt gtgggaataa agtgccgcca agggttccgc atcgccattc
     3421 agcatggcat tgagcacgtc gaaccactgg ctgacagcgg cgcgtatggc aatttcgtca
     3481 tcgcttggca tggggcctcc tcccgtggga actttggcgt attgagt
//
LOCUS       DBNHVU010000086         5683 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1892365, whole genome shotgun sequence.
ACCESSION   DBNHVU010000086 DBNHVU010000000
VERSION     DBNHVU010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5683)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5683)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5683
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1892365"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="AC16Y7_03215"
     CDS             <1..543
                     /locus_tag="AC16Y7_03215"
                     /inference="COORDINATES: protein motif:HMM:NF015509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR5708422.1"
                     /translation="MTLILLTLNDATAPAAKPGSGKAAAAAPEAGIGEKIFSALKQPV
                     VWLPLLGFALVAIDVEVPKVFNDSLMLLGHAAAGVALFTSGIILAGYKITFTMPVGVL
                     VLIKNVVQPALVFAGMMWLGYSDPILGQTVVTTALPAVTLVVMLAVQYRVAIPETASA
                     LLISTFGSLVTLSVFIWFLL"
     gene            complement(558..1091)
                     /locus_tag="AC16Y7_03220"
     CDS             complement(558..1091)
                     /locus_tag="AC16Y7_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5708423.1"
                     /translation="MPRNASPGLRDDLVAQIGSLRAFAVSLCGDRERADDLVQETLCK
                     AWKHLDSFKEGTNLKAWLFTILRNTFFSERRKRKREVEDADGNLAARLSSLPEQHGHM
                     DMKDFQEALSKLPDDQREALILVGAAGFSYEEAAEISGCAVGTIKSRVNRARNRLAES
                     LELGEEDEAPSAMTKTA"
     gene            complement(1092..1295)
                     /locus_tag="AC16Y7_03225"
     CDS             complement(1092..1295)
                     /locus_tag="AC16Y7_03225"
                     /inference="COORDINATES: protein motif:HMM:NF037848.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NepR family anti-sigma factor"
                     /protein_id="HIR5708424.1"
                     /translation="MTVDTTKRVERHDGPDAAESPPGEGMSPDLQAHIGRQVRAMFDE
                     VAEEPIPDHLLRLLQDLDRGGEK"
     gene            1562..2371
                     /locus_tag="AC16Y7_03230"
     CDS             1562..2371
                     /locus_tag="AC16Y7_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5708425.1"
                     /translation="MTLSQTIAPHLPFLRRFARALAGTQKAGDAYVAATLQVLVEDPS
                     LFDKNLDPRVALYRTFLGLWNSVGINLKTDIAEGGTTSVDRKLEQITPLPRQAFLLCS
                     VEGFAPEDAAVVLNTSASNVEALIDDAGREIAAQVATDVLVIEDEPMIAMDLESIVEG
                     LGHRVTGVARTHSEAVDAVSKEKPGLVLADIQLADGSSGLDAVNEMLGSFEVPVIFIT
                     AYPDRLLTGERPEPAFLITKPYQPDTVKAIVSQALFFERRAKLKDEPQAGA"
     gene            complement(2383..2538)
                     /locus_tag="AC16Y7_03235"
     CDS             complement(2383..2538)
                     /locus_tag="AC16Y7_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HIR5708426.1"
                     /translation="MGSTVLLIILIGVFLAVLPVWPYSRAWGPFPSGLVGLIIVLMLA
                     LLFFNLL"
     gene            2690..3034
                     /locus_tag="AC16Y7_03240"
     CDS             2690..3034
                     /locus_tag="AC16Y7_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5708427.1"
                     /translation="MSAQSILVVEDEFLIALDIVAALEQAGVAVAGPVSTVGDALAAI
                     EQQPLRGVLLDAHLGGESAARIADALTARRLPFAFVSGYGRESLPQAYQNAPLVRKPF
                     TDQDLLTAIAAF"
     gene            3051..3209
                     /locus_tag="AC16Y7_03245"
     CDS             3051..3209
                     /locus_tag="AC16Y7_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HIR5708428.1"
                     /translation="MLLYLAIMFAVVALVAGVFGFGGVASAAGGIARILFFIFLVVFL
                     VTLIAAIV"
     gene            3241..3912
                     /locus_tag="AC16Y7_03250"
     CDS             3241..3912
                     /locus_tag="AC16Y7_03250"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019174.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5708429.1"
                     /translation="MDELKLTRRELEERTKELAEAKQRLEVTLRELTHRSKNLLTVIN
                     AIARQTAARTRSVDDFLDRFGARLYAIGMSHDLLVADDWEGASLRMLVEQQLKTHADG
                     GRIAIEGDDVMLKPEAVHNLGLALHELTANAEKYGSLSDPQGAIRIAWNLCEDDKSLK
                     LIWQEMNGPEVREPERSGFGRAMLENVVSKALAGVVTLSFPAHGVHCEIVIPAGHVSD
                     VTPRP"
     gene            4090..4593
                     /gene="msrB"
                     /locus_tag="AC16Y7_03255"
     CDS             4090..4593
                     /gene="msrB"
                     /locus_tag="AC16Y7_03255"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIR5708430.1"
                     /translation="MVTRRGILLGTAGLAAVAGAGIGAAKWWGSQATAATDKTFEVVK
                     TPEEWRKILTPQQYAILRDADTERAWTSPFNKEKRKGTFACAGCDLPLFASETKFESG
                     TGWPSFYEPIDGAIGVEKDFSWGMSRDEVHCRRCGGHLGHVFNDGPKPTGLRYCINGL
                     ALKFLPA"
BASE COUNT         1071 a         1726 c         1764 g         1122 t
ORIGIN      
        1 atgacgctca tattgctgac cctgaacgac gccacggcac ccgccgcgaa gccaggttcg
       61 ggcaaggccg cggcggcggc acctgaagcg ggcatcgggg agaagatctt tagcgccctg
      121 aagcaacctg ttgtgtggtt gccgcttctg gggttcgcgc tggtcgcgat cgatgtcgaa
      181 gtgccgaagg tcttcaacga ctccctcatg ctgctcggac atgccgccgc gggcgtcgcg
      241 ctattcacct cgggaatcat tctggccggc tacaagataa cattcaccat gccggtaggc
      301 gtgctcgtct tgatcaagaa cgtcgtgcag ccggcgctcg tgtttgcggg catgatgtgg
      361 ctcggctaca gcgacccgat cctcggacag accgtcgtga ccaccgcgct gccggctgtc
      421 acgctagtcg tcatgctcgc cgttcaatat cgcgtggcca ttccggagac cgcctccgcg
      481 ctgttgatca gcacgttcgg ctcgctcgtg acgttgagcg tctttatctg gttcttgctc
      541 tgagcgggga aggggggtca ggccgttttg gtcatcgccg agggagcctc gtcctcctcg
      601 ccaagttcaa gggactcggc gaggcggttg cgcgcccggt tgacccggct cttgatcgtg
      661 ccgacggcgc agccggaaat ctccgcggcc tcttcgtagg aaaaacccgc cgcgccgacg
      721 agaatgagcg cctcgcgctg gtcgtcggga agcttggaga gcgcttcctg gaaatccttc
      781 atgtccatgt gcccgtgctg ctcagggagc gaggacaggc gcgcggcgag gttgccgtcg
      841 gcgtcctcca cctcgcgttt gcgtttgcgg cgttcggaga aaaacgtgtt gcgcaggatc
      901 gtgaacagcc aagccttcag gttcgtccct tccttgaagg agtccagatg cttccatgcc
      961 ttgcacagcg tttcctgaac gagatcgtcc gcgcgctccc gatcgccgca cagcgatacg
     1021 gcgaaagccc gcaggctacc gatttgggcc acgaggtcgt ccctcaggcc cggggaagca
     1081 ttacgtggca attatttctc cccgcctcgg tcgaggtcct gaagcaagcg caaaagatga
     1141 tctggaatgg gctcttcggc gacttcgtcg aacatcgcgc gcacctgacg accgatatgg
     1201 gcttggaggt ccggggacat gccttctccc ggcggactct cggcagcatc ggggccgtcg
     1261 tgccgctcca cacgcttggt agtatctacc gtcatgcgcc tttgggcctt cttcttgtca
     1321 ctacggtcca cggcgggtct ttctccgctc tgctgccagg actggcagtc tagcggattc
     1381 tgccgcgctt ctgacccaaa aaacacgcta aggttcagat ggttccagga tcggggaggg
     1441 gagtgggcgt gcagcttgca ttcttcgctg gacgcaccgg ctgaatctgt caccctccgg
     1501 cccggttcat gcgaataagt gtgcaccctt ggtggtgccc aaggagaaga caagaaaatc
     1561 catgacgctg tcgcaaacga tcgctccgca tcttcccttt ctgcgccgtt tcgcccgcgc
     1621 gctcgccgga acgcaaaagg ccggtgatgc ttacgtggcc gcgaccttac aagtcctggt
     1681 cgaggatcca tcactgttcg acaagaacct cgatccgcgc gtggccctct accggacgtt
     1741 cctcggtctt tggaattcgg tcggcatcaa tctgaaaacg gacatcgccg aaggcggcac
     1801 gaccagcgtc gaccgcaagc tcgagcagat cacgccgctt ccaagacaag cttttctgct
     1861 gtgctccgtc gaaggcttcg cgccggagga cgcggctgtc gtcctcaaca ccagcgcgag
     1921 caatgtcgag gcgttgatcg acgacgcggg ccgcgagatc gccgcccaag tcgcaacgga
     1981 cgtgctggtc atcgaggacg agccgatgat cgccatggat ttggaaagca tcgtggaggg
     2041 gctcgggcat cgcgtcacgg gcgtggcgcg cacccatagc gaggcggtcg atgccgtgtc
     2101 gaaggaaaag ccggggctgg tgctggcgga cattcagctc gccgacggga gctcgggcct
     2161 cgatgccgta aacgagatgc ttggctcgtt cgaagtgccg gtgatcttca ttaccgctta
     2221 tcctgaccgg ctcctcaccg gcgagcggcc ggagccggca ttcctgatca ccaagcccta
     2281 ccagcccgac acggtgaagg cgattgtctc gcaggcgctg ttcttcgagc ggcgcgccaa
     2341 gctcaaggac gagccgcaag ctggcgccta gacggccggc agctagagca agttgaagaa
     2401 caacagtgcg agcatgagga cgatgatcaa tccgacaagg cccgatggga acggtcccca
     2461 ggcgcggcta tagggccaca ccgggagaac ggcgaggaag acgccgatca ggatgataag
     2521 aaggactgtc gagcccattg ctccccctta ggatgcctgt cgtgcgccga ccatagctca
     2581 ttttgcgcgc caagccagac gcacaaattg gcgcgtcacc cggacgcatg aaacggacgc
     2641 aagggatgtg cgttccaact ccggtagcgt tgagtaaagg ggagaccgga tgagcgcgca
     2701 gtccatactt gtcgttgagg acgaattcct catcgctctc gacattgtcg ccgcactcga
     2761 acaggcgggc gtcgcggttg ccggtcccgt gagcaccgtt ggcgatgctc ttgccgccat
     2821 cgaacaacag ccattgcgcg gtgtattgct agacgcgcat ctgggcggtg aatcggccgc
     2881 gcgcatcgcc gatgcgttga ccgcgcgccg cttgccgttt gctttcgtca gcggctatgg
     2941 acgcgaaagc ctgccgcagg cctatcaaaa cgcgccgctc gtcagaaagc cgttcaccga
     3001 tcaagatctt ctgactgcga ttgccgcgtt ctgagtggga ggagtaagag atgttactgt
     3061 acttggcgat tatgttcgcc gtcgtggccc tggtggccgg cgtttttggt ttcggcggcg
     3121 tggcgtcggc cgcaggtggc atcgcgcgca ttcttttctt tatcttcctg gtggtcttcc
     3181 tcgtgacgct gatcgcggcg atcgtctagc gttgcgtgtg gctgcagcag agacagaacc
     3241 atggacgagc tcaaactaac gcgccgggag ctcgaagagc gcaccaagga attggccgag
     3301 gcgaagcagc gtctcgaagt caccctgcgt gagctgaccc atcgctccaa gaatttgctg
     3361 accgtgatca acgccatcgc gcggcagacc gccgcgcgca ccaggtcggt cgacgacttt
     3421 ctcgatcgct tcggcgcgcg cctctacgcc atcggcatgt cgcacgacct tcttgtggcg
     3481 gacgattggg agggcgcgtc gttacgcatg ctcgtcgaac aacaactcaa gactcacgcg
     3541 gacggcggtc gcatcgccat cgagggcgac gacgtcatgc tcaagcccga ggcggtgcac
     3601 aatcttggac tcgctttgca tgagctcacg gccaacgcgg agaaatatgg atcgctgtcc
     3661 gatccgcaag gcgccatccg cattgcctgg aacctctgtg aggacgacaa gtcgctgaag
     3721 ctgatctggc aggagatgaa cggacctgag gtgcgcgagc ccgagcgttc gggcttcggg
     3781 cgggccatgc tggagaacgt cgtgagcaag gcgctcgccg gcgttgtcac cttgtcgttc
     3841 cccgcgcacg gcgttcactg tgagatcgtt atccccgcgg gccacgtgag cgatgtgacg
     3901 ccgcgcccgt gaggcgcccc ttcgcgcgcc cgaaataagc ccgtaatcgc gccccaatag
     3961 cccgtaaatc tcagcctaat aaagcgctcg tgaacacgcg tgcgcgtaag ttgagttgat
     4021 gcgtggacgg aagtccctag attcggatgg agaactcagc ccgaggacgg gcgattagga
     4081 ggatccatca tggtcacacg acgaggcatt ctgctgggca ccgcgggtct cgccgcggtc
     4141 gcaggcgccg gtatcggtgc cgcgaaatgg tggggcagtc aggcgacagc cgccaccgac
     4201 aagacgttcg aggtggtcaa gacgccggag gaatggcgga aaatcctgac gccgcagcaa
     4261 tacgccattc tgcgcgatgc ggacacggag cgtgcttgga cgagcccctt caacaaggag
     4321 aagcgcaaag gcaccttcgc ttgcgccggc tgcgacctgc cgctgttcgc ctctgagaca
     4381 aagttcgaat ccggtacggg ttggcccagc ttctacgaac ccatcgacgg tgccatcggc
     4441 gttgagaagg atttcagctg gggcatgtct cgcgacgagg tccattgccg ccgctgcggc
     4501 gggcatctcg gccacgtgtt caacgacggc cccaagccca cgggcttgcg ctactgcatc
     4561 aacgggctcg ctctcaagtt cctccccgcc tagcggcctc ccttggcgcg gctccggttg
     4621 tggggccgcg ccgccacgcg ccactgatcc aaatcggaaa aagttggaac gattctcgcc
     4681 gggccgtgtt tgtgacaccg gcgggcaaag taccgtgtat cccgcctcgc cgtacgggtt
     4741 acgggggtga aggggtcggt ttcgaccctt tcgctcgcct ccgccggcct ccctcttttc
     4801 ctcacaacat catccaagca gacgcaacat agggctgccc catgtccgca cccatggggg
     4861 acgtcagcgc gcatttgcat tttcggagtg agagtgagtt ggctcccggc ttctctgagg
     4921 ctcaggcctg ggctccggtt agcaccgaca cccacaggcg tcgtcacagt cgttggattg
     4981 ctgtgatggc attgcccgcg tcagtgaaag cgggacgtaa aggccgaccg cctgaccccg
     5041 gcctacctcg aatgaggttg tcctaatcca gctgtgccga cgcagaagcc ctgagcacgg
     5101 tttattttcc agtggattga tctcttgaat gagctctccg aaatggcccg gtctccgcta
     5161 gcagccgaga taaacttttt ggcttcactt gcgtcttatt gggagaccga attggggctc
     5221 caactcaggc tcgcccggat agcaccgagg caacgtgata gaacacgaca gcaagaaggg
     5281 tattttgccg atatcgcgcg agcagctgct gagctgctgc ctgagaagca catggcagac
     5341 tctttggaac ctgcgctcgt gccctaggtt gtaggcgctg atattatcaa ccccgatgtc
     5401 gttaccctag atgaccacga tgttgggctt gtccgtctgg gccttggcag gtccgacagt
     5461 cgcgaacacg gctattcccc acccaaagca tagttctaac gaacaaagat cgtctcattg
     5521 cgttagagca tcccggttag aacaatgaga gtaagtacat atgagcaaga tagtcagagc
     5581 tttgaccctc agcgtcatgc tgccgtttgc agctattgcg acgacatccg cgagtgccga
     5641 ggatgatcca agtgctgcga aaggcgctgt gaagggcggt gtg
//
LOCUS       DBNHVU010000087        10394 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_627735, whole genome shotgun sequence.
ACCESSION   DBNHVU010000087 DBNHVU010000000
VERSION     DBNHVU010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 10394)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10394)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10394
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_627735"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="AC16Y7_03260"
     CDS             <1..457
                     /locus_tag="AC16Y7_03260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HIR5708431.1"
                     /translation="RHRAREARRDDQVRAAGGGALIDSNNLRFQSRWRHSIASMVPVA
                     YVLGPFRNHLSIGRIHHLHHVNWGKEGDCNFGLFLTLWDRVFGTFQAEPSRSIEAHDM
                     GIDEVPNFPKSYIEQLIFPFIYKPGAGEPDLNKAETPAHEARRIVDAAE"
     gene            complement(550..1815)
                     /gene="rho"
                     /locus_tag="AC16Y7_03265"
     CDS             complement(550..1815)
                     /gene="rho"
                     /locus_tag="AC16Y7_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR5708432.1"
                     /translation="MQEMKLQDLKTKSPTELLSFAEEVEVENASAMRKQELMFAILKQ
                     LAAREVDITGTGVVEVLQDGFGFLRSPDSNYLAGPDDIYVSPSQIRRFGLRTGDTAEG
                     QIRSPKDGERYFALLKVNTINFEEPEKQRHKIHFDNLTPLYPDEWLEMETDDPTKKDQ
                     TGRVIDIVAPIGKGQRGLIVAQPRTGKTVILQNIAHAITANHPECYLIVLLIDERPEE
                     VTDMQRSVKGEVVSSTFDEPASRHVQVAEMVIEKAKRLVEHGRDVAILLDSITRLGRA
                     YNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDEVI
                     FEEFKGTGNSELVLDRKVADKRVFPAIDIARSGTRKEELLVPGDQLKKMYVLRRILNP
                     MGTMDAIEFLNDKLKQTKNNAEFFDSMNT"
     gene            complement(2072..2524)
                     /gene="hemJ"
                     /locus_tag="AC16Y7_03270"
     CDS             complement(2072..2524)
                     /gene="hemJ"
                     /locus_tag="AC16Y7_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase HemJ"
                     /protein_id="HIR5708433.1"
                     /translation="MTVAMPAEILSYIKACHIIAVIAWMAGVLYLPRLFVYHAASKPG
                     SEQSETFKIMERRLLRAIVTPAMILAWIFGLILAFSPLIDWSRDFWLHAKLVLVVILS
                     GFTGLLARWTRDFAADRNTHSQRFYRIANEVPTLLMIAIVILVVVRPF"
     gene            complement(2532..3578)
                     /gene="hemE"
                     /locus_tag="AC16Y7_03275"
     CDS             complement(2532..3578)
                     /gene="hemE"
                     /locus_tag="AC16Y7_03275"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HIR5708434.1"
                     /translation="MSEALSPSSPFLAALQGETNAPPPLWLMRQAGRYLPEYREVRRT
                     VPSFLDLCLTPALAAEVTLQPLRRFAFDASIVFSDILVVPFALGQQVTFVEGEGPKLD
                     PVRDGAAIARLEFAGASERLAPVYETVERVVAGLPDGVPLIGFCGAPWTVATYMVEGA
                     GSKDQAAARRLAYGDPEAFQALIDTLIEASSAYLIGQVEAGCRAIQIFDSWAGSLAED
                     QFARWCIAPTKAIVTRVKAAHPDIPVIGFAKGAGPSSEVYAAETGIDAIGCDTSLPLG
                     FIRNRLQAHLLPVQGNLDPVLLLAGGEALETRVRTILETLGEGPFVFNLGHGILPETP
                     IENVERLVALVKGA"
     gene            4006..4839
                     /locus_tag="AC16Y7_03280"
     CDS             4006..4839
                     /locus_tag="AC16Y7_03280"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HIR5708435.1"
                     /translation="MDIQRTFHLHLVSDATGETLATIAKAVSVQYAMVRATEHLHPLV
                     RSRRELDRILKEVEAQPGIVLYTLFNREMADALEQSCKDLNIPCIAPLKHVLQVFDSY
                     LGMASTPTVGGQHQLDADYFRRIEALNFTMLHDDGHLPENLDDADIILLGISRTSKTP
                     TSIYLANRGFKTANVPLVPGMTPPPQIEEETKAFVVALVASPERIAQVRHNRVIEHAH
                     GHLADYVDRDAIAAEIAQTRQLCARHGWPIIDVTRRSIEESAAAIIRLYHDREAPALE
                     A"
     gene            4836..5459
                     /locus_tag="AC16Y7_03285"
     CDS             4836..5459
                     /locus_tag="AC16Y7_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HIR5708436.1"
                     /translation="MTGFAQTGCPPLILASTSKTRARLLESAGLAFLCEAPGVDESTM
                     RQAISGEDALGPHDVAEVLARAKAEAVSDVTQGAYVIGGDQVLCFKDRIISKPDSMEA
                     ARAQLLDLNGKTHQLHTAVALATNGETVWAYTDVTTLTMRKLSPEFIGRYLAAAGDAV
                     LSSVGAYQIESIGIQLFERIDGDFFSILGLPLLPLLDALRRQGAIEG"
     gene            5462..6298
                     /locus_tag="AC16Y7_03290"
     CDS             5462..6298
                     /locus_tag="AC16Y7_03290"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR5708437.1"
                     /translation="MSPRACVIGWPVEHSRSPVIHRYWLDQYGIDGAYEKEAVRPEDL
                     ERFVQQLWTNGYVGANVTLPHKEAALRAARSADEAAIAIGAANTLWLDPDGALHASNT
                     DAYGFMTNLDAKAPQWRAAKGDVIVLGAGGAARGILYGLLKAGVPRVLLTNRTRERAE
                     TLREAFGPKVEVIDWAARNEALAGCGLLVNATSLGMTGHKPLIINIAALPNDAVVSDI
                     VYVPLETPLLRDARTRGLATVDGLGMLLHQAVPGFERWFGVRPEVTPELTAHVVATLG
                     EG"
     gene            6298..6921
                     /gene="coaE"
                     /locus_tag="AC16Y7_03295"
     CDS             6298..6921
                     /gene="coaE"
                     /locus_tag="AC16Y7_03295"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217223.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR5708438.1"
                     /translation="MLVVGLTGGIGMGKSSAAAHFRERGVPVFDADAYVHALYEGAAV
                     PAIEAAFPGTVRDGRVDRARLAREIAGAPQRLAELEAIVHPLVVQAEIDFLCAEEKKA
                     TTLAVLDIPLLFETGAESRVDVTIALSTPAAVQRTRVLTRPGMTKEKFEALIARQLTD
                     AERRARADLVVDSGSTLENLHTQLDRLLESLQTRDGTVMEQLRSQKS"
     gene            6938..7627
                     /gene="dnaQ"
                     /locus_tag="AC16Y7_03300"
     CDS             6938..7627
                     /gene="dnaQ"
                     /locus_tag="AC16Y7_03300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HIR5708439.1"
                     /translation="MREIILDTETTGLDAFDGDRIVEIGCVELVNSIATGRTWHCYLN
                     PERDVPEQAFKVHGLSTDFLSDKPLFEERADAFLEFVEGSMLVIHNASFDFGFLNAEL
                     ERASRPLLNWDRVIDTLALARRRHPGAPASLDALCRRYGIDLSERDLHGALLDCRLLA
                     SVYVELVGGHQARLEFATNGAQAAILQARESAVRTRPAPLAPRVSEAEHEAHSAFVAT
                     LGEDALWLRYA"
     gene            complement(7646..8212)
                     /gene="secB"
                     /locus_tag="AC16Y7_03305"
     CDS             complement(7646..8212)
                     /gene="secB"
                     /locus_tag="AC16Y7_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HIR5708440.1"
                     /translation="MARKPKDDNAKRTNGDGPTPKEAPATAGGSGPSTAGEGANARPQ
                     LNVVAQYVKDLSFESPGAPQTLQGPGENPQLKVGVNVNAGARGEDIYEVAVNIEAHAK
                     SDVGVIYNAELVYAGVFRIVNVPQDMLQPIMFVDCPTILFPFVRRVLSDVVRDGGFPP
                     LMLDPIDFARLYAQNMAGDQKAAQAQPN"
     gene            8498..9223
                     /locus_tag="AC16Y7_03310"
     CDS             8498..9223
                     /locus_tag="AC16Y7_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HIR5708441.1"
                     /translation="MSEVFDIYTLLFLVLAVVIFIRLRGVLGRRTGNERPPYDPYTRP
                     DAKQAGTPEGTVVALPRGRTGKPAGENAGPTQEDIDERLERYATKDSPLGKSLTALIK
                     VDPSFDPGQFLDGAKVAYEVIVGAFADGDEKTLKQLLGDDVLDGFMRAIDEREGRGEQ
                     AQTTLVGIDKADILEAEIKDKQAFVTVKFVSELISVTRDGEGDVVDGDPTKVREVTDI
                     WTFARGIDSRNPNWKLVATEAAN"
     gene            9231..10316
                     /gene="mltA"
                     /locus_tag="AC16Y7_03315"
     CDS             9231..10316
                     /gene="mltA"
                     /locus_tag="AC16Y7_03315"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HIR5708442.1"
                     /translation="MTRRGPAADPFDRDFPPLSFDMLEGWEDDDHLAAFRAFLKSCEH
                     DALSDLEKASFPREEALALGPDIGKDAAREFFETHFTPRCRYDSRLDDGFVTGYYEPM
                     VRGARERSAQYCVPVYGRPDDLVMLSDDLYRAAANDRISARRDCGDALEDYFTRAQIE
                     DGALKDRGLEALYLDDWIETFFMHVQGSGLVQLDDGTEVRLAYDGKNGHPYTSIARQL
                     VDRREVLPKDIDMEKVKAWLRADPERGRALMQENQSYIFFRELERDEGRDGPLGAEGV
                     PLTAGRSLAVDPVYTPLGCPVYLLAANLPSERGPEFARLMIAQDVGSAIRGPERGDIF
                     WGSGEAAGAIAGTTRHLAVFIFLLPNP"
BASE COUNT         1945 a         3166 c         3325 g         1958 t
ORIGIN      
        1 ccgtcaccga gcccgagaag ctcgtcgaga cgatcaagtc cgcgcggcag gtggcggtgc
       61 attgattgac tcaaataacc tcaggttcca gagtcgatgg cggcactcga tcgcgtccat
      121 ggtgcccgtc gcctacgtgt tggggccgtt ccgcaatcac ttatcgatcg gccggatcca
      181 ccacttgcac cacgtcaact ggggcaagga gggcgactgc aatttcggcc tgttcctaac
      241 gctatgggat cgcgtgttcg gcaccttcca agcggagcca tccagatcga tcgaagcgca
      301 cgacatgggt atcgacgagg tgccgaactt ccccaagtcg tatatcgaac agttgatctt
      361 cccgttcatc tataagcctg gcgccggcga accggacctc aacaaggccg agacaccggc
      421 ccacgaggcg cgccgcatcg tcgacgcggc cgagtaagcc agccgcaacg catttgcaat
      481 caaccaaggt cagtacttgc cggattgccg ggtcaagccg ggcaatgacg acgttgccgc
      541 cgtgctgccc taagtgttca tcgagtcgaa gaactcggcg ttgttcttgg tctgcttcag
      601 cttgtcgttg aggaactcga tcgcgtccat ggtgcccatc gggttgagga tgcggcggag
      661 cacgtacatc ttcttgagct gatcgccagg aaccaaaagt tcttccttgc gggtgccgga
      721 ccgggcgatg tcgatggccg ggaagacgcg cttgtcggcg actttgcggt cgagcacgag
      781 ctcggagtta ccggtgccct tgaactcttc gaagatgacc tcgtccatgc ggctgccagt
      841 gtcgatcaag gccgtcgcga tgatggtgag cgaaccgcct tcttcgatgt tgcgggcagc
      901 gccaaagaaa cgcttcggcc gctgcaaggc gttggcgtcg acgccaccgg tcaacacctt
      961 gcccgaggac ggcaccacgg tgttgtaggc gcggccgaga cgcgtgatcg aatcgagcag
     1021 gatggccacg tcgcggccat gctcaaccaa gcgcttcgcc ttctcgatga ccatctcggc
     1081 cacctgcacg tgacgcgacg ccggctcgtc gaacgtcgag gacacgacct cgcccttcac
     1141 cgagcgctgc atgtcggtca cttcttccgg ccgctcgtcg atcaggagca cgatcagata
     1201 gcactccgga tgattggcgg tgatggcatg ggcgatgttc tgaaggatca ccgtcttacc
     1261 ggtgcgcggc tgcgccacga tgaggccacg ctggcctttg ccgatgggcg ccacgatgtc
     1321 gatgacgcgg ccggtctggt ccttcttggt cggatcgtcc gtctccatct cgagccattc
     1381 gtcgggatag aggggcgtca gattgtcgaa gtgaatcttg tggcgctgct tctcgggctc
     1441 ctcgaagttg atcgtgttga ctttgagaag cgcgaagtag cgttcgccgt ctttcggact
     1501 gcggatctgg ccttcggcgg tgtcgccagt gcgaagacca aagcggcgga tctgcgaggg
     1561 cgagacgtag atgtcgtcag gcccggcgag atagttcgag tcgggcgacc gcaagaagcc
     1621 gaagccgtct tgcagcacct cgacaacgcc cgtaccggtg atgtcgacct cgcgggcggc
     1681 gagttgtttc aggatcgcga acatgagctc ttgcttgcgc atggcgctcg cgttctcgac
     1741 ttcgacctcc tcggcgaagc tcaggagctc cgtcggcgat ttggtcttta aatcttgcag
     1801 cttcatttct tgcatgcgct gccactccaa aatggaaaag cgttgattgt tgatgtttcg
     1861 atatcgcgct tcgttcgcgc gggtatcagg gaaggggtgg gatatgcctc ggcgggatgg
     1921 cgataagcgg cgcctgcccc agtcccggtc gtccgatctt ttttgaggaa ggccggaccg
     1981 cgagaggcgt cccggaatgg atgcgagtcg ctttatagca gagcccacct gcatacggaa
     2041 taggcttcaa gcctccttgt cacgctcttt gtcagaaagg ccgcaccacc accaggatga
     2101 cgatagcgat catcaggagc gtcgggacct cgttggcgat gcggtagaaa cgctgggagt
     2161 gcgtgtttcg atccgcagcg aaatcgcgcg tccaccgggc caagagtccc gtgaagcccg
     2221 acaggatgac gacgaggacc agtttggcgt ggagccagaa atcccgcgac cagtcgatca
     2281 ggggcgaaaa ggccagaatc aggccaaaaa tccaggccag gatcatggcc ggggtgacga
     2341 tggcgcgaag gagccgacgc tccatgattt tgaaggtctc ggactgctcg gagcccggct
     2401 tggaggccgc gtgatagacg aaaagccgcg gcagatagag cacgcccgcc atccaggcga
     2461 tgaccgcgat gatgtggcag gccttaatat aggaaagaat ttcagcgggc attgcaacgg
     2521 tcatggcggc cttaagcgcc cttcaccagg gcgaccagtc tctcgacatt ctcgattggg
     2581 gtctccggca agatgccatg acccagattg aacacgaagg gcccctcacc aagcgtctcc
     2641 aggatggttc tgacgcgggt ctccagggcc tcaccgcccg ccagcagcag gaccgggtcg
     2701 agattgccct gcacaggcaa caggtgcgct tgcagccggt tccggatgaa ccccaaggga
     2761 aggctggtat cgcagccaat ggcgtcgatc ccggtctcgg ccgcatagac ctcacttgag
     2821 ggccctgcgc ctttggcgaa gccgatcacg gggatatcag ggtgggcggc cttcacgcgg
     2881 gtaacgatgg ccttggtagg ggcgatgcac cagcgggcga attggtcttc cgcgaggctt
     2941 cccgcccagc tgtcgaagat ctggatcgcg cggcaacctg cctccacctg gccgatcaga
     3001 taggcgctgg aggcctctat cagcgtgtcg ataagggcct ggaaagcttc agggtcgcca
     3061 taagccaagc gccgcgccgc cgcctggtcc ttgctgccgg cgccttcgac catgtaggtc
     3121 gccaccgtcc agggcgcccc gcagaagccg atcaacggga ccccgtctgg cagtcccgcc
     3181 accacgcgct ccacggtctc atagaccggt gccagccgct ctgaggcgcc tgcaaattcc
     3241 agtctggcga tcgccgcccc atcgcgcacc ggatcgagct tgggcccttc gccctccacg
     3301 aacgtgacct gctgacccag tgcaaagggc acgaccaaga tatcggagaa cacgatggac
     3361 gcatcgaagg caaaacggcg aagcggctgc aaggtcactt cggcggccaa tgccggcgtc
     3421 agacagagat cgagaaagga gggaacggtc cgccgtacct ctcggtactc gggcagatag
     3481 cggcccgcct gacgcatcag ccacaagggc gggggtgcgt tcgtctcgcc ctggagagcc
     3541 gccagaaacg ggctcgatgg cgataaggcc tcagacatgt gtctctcact aaaactaata
     3601 aagagattcc ttaagagagt ttcttttatt tctgttaggg cgtggatagc ggacattcac
     3661 gatcatcacc agtctgtcga caggttaggt aagcgccagt ttgaaagttt gaagattgaa
     3721 aggtggggcc gtggccggct tgccttgaaa agcgacttaa cagtctggtt cgctatggct
     3781 tctagatagc ccgcaggtct ggattacatt gggatggctt gtgggcatct accgtttaga
     3841 gctggggatc aaaaagggta ttgtgggcct ctgcggaaca gaggtcatgt tttcgacaca
     3901 agcgaatctg tgaggaagta ggactcataa cttctggtaa gtggggacag cctcagggcc
     3961 cgcgaagtta cgcccgaaag cctgacggtt atccaaaggc catacatgga tatccaacga
     4021 acgtttcatc tgcatttggt ctccgacgcg accggcgaaa cgctggcaac aatcgccaag
     4081 gcggtcagcg ttcagtacgc gatggtgcgc gcgacggagc atctgcatcc tctggtgcgc
     4141 agccggcgcg agttggaccg catattgaag gaggtggaag cgcagccggg catcgtgctc
     4201 tacacgctgt tcaaccgcga gatggccgat gcattggagc aatcgtgcaa ggatctcaat
     4261 attccttgca tcgcgccatt gaaacatgtg cttcaagtgt tcgattcgta tctcggcatg
     4321 gcctcgacgc cgaccgtggg cggacaacac cagctcgacg ccgattattt ccgccgcatc
     4381 gaagctttga acttcaccat gctgcacgac gacgggcacc tgccggagaa tctggacgat
     4441 gccgacatca tcttgctcgg catcagccgc acgtctaaga cacccacgag catctatctc
     4501 gccaatcgtg gcttcaagac cgccaatgtg cccctcgttc ccggcatgac accgccgccg
     4561 cagatcgaag aggaaacgaa ggcgtttgtc gtggcgctgg tggcgagccc ggaacgaatc
     4621 gcgcaagtgc ggcacaaccg ggtcattgaa catgcccacg gtcatttggc ggactacgtc
     4681 gaccgcgatg ccatcgccgc ggagatcgcc cagacgcggc agctttgcgc gcgccatggc
     4741 tggccgatca tcgacgtgac gcgccgctcc atcgaggagt cggcggcggc gatcatccgc
     4801 ctttatcacg accgcgaagc gccggcgttg gaagcatgac gggatttgcg caaaccggct
     4861 gtccgccgct gatcctggcg tcgaccagca agacgcgcgc gcggcttttg gagtccgccg
     4921 gccttgcctt tctgtgcgag gcgccggggg tcgacgaaag caccatgcgc caggcgatca
     4981 gtggtgagga cgcgctcggt ccccatgatg tggcggaggt gctggcgcgc gccaaggccg
     5041 aggcggtaag cgatgttacg caaggcgcct atgtcatcgg cggcgatcag gttctttgct
     5101 ttaaggaccg gatcatctcc aagccggaca gtatggaggc agcccgcgcg cagctccttg
     5161 acctcaacgg caagacgcac caattgcaca ccgccgtcgc gctcgccaca aacggtgaga
     5221 cggtgtgggc ctatacggac gtgacgacgt tgaccatgcg caagctttcg ccggaattca
     5281 tcggccgcta tctcgccgcc gccggcgatg cagtcttgtc gtctgtcggc gcttatcaga
     5341 tcgaatcgat cggtattcag ctgttcgaaa ggatcgacgg cgacttcttc tccattctcg
     5401 gcctgccgct gctgccgctc ctcgacgccc ttcggcgcca gggcgcgatc gagggttgag
     5461 catgagcccc cgcgcttgcg tcatcggctg gcccgtcgag cactcccgct ccccggtaat
     5521 ccaccgctat tggctcgacc aatacgggat cgacggcgct tacgaaaaag aggcggttcg
     5581 ccctgaagac ttggagcgct tcgtccagca gctttggacc aatggctatg tgggcgccaa
     5641 tgtcacgctg ccccacaagg aggcggcgtt gcgtgcggct cgctctgccg atgaggcggc
     5701 gatagcgatc ggcgcggcga acacgctgtg gctggacccc gatggcgctc tccacgccag
     5761 caacaccgac gcttatggtt tcatgaccaa tctcgacgcg aaggcgccgc aatggagagc
     5821 ggccaagggc gacgtcatag tgctcggcgc gggcggcgcc gcgcggggca ttctctatgg
     5881 cctcctgaag gcgggtgtgc cccgcgtcct tctcaccaac cgcacgcggg agcgggccga
     5941 gacgctgcgc gaagcttttg gcccgaaagt cgaggtcatc gactgggcgg cacgaaacga
     6001 ggcgcttgcc ggctgcggtc tcctggtcaa tgcgaccagc cttggcatga cgggccacaa
     6061 accgctcatc atcaacatcg ccgcgctgcc gaacgatgcc gttgtgtccg acattgttta
     6121 tgtgccgctg gaaacgccgc tcctgaggga cgcccgcacg cgcggtctgg caaccgtcga
     6181 tgggcttggc atgttgctgc accaggccgt gcccggcttc gagcgctggt tcggcgtccg
     6241 gccggaagtc acaccagaac tgaccgcgca tgtcgtggcc acgttgggag agggctaatg
     6301 ctggtggttg gtctcaccgg cggtatcggc atgggcaaga gctcggccgc cgcgcatttc
     6361 cgcgaacgcg gcgtccccgt tttcgacgcg gacgcctatg tgcacgcgct ctacgaaggg
     6421 gcggcggtgc cggccatcga ggcggcgttt cccggcacgg tccgcgacgg ccgggtcgac
     6481 cgcgcgaggc tcgccagaga gatcgcaggc gcgccgcagc gcctcgccga gctggaagcc
     6541 atcgtgcatc cgcttgtggt gcaggccgag atcgattttc tctgtgcgga agagaagaag
     6601 gcaacgacgc tcgccgtgct cgacatcccg cttctgttcg agacgggcgc ggagagccgc
     6661 gttgatgtca ccatagcgct cagcacgccg gccgccgtgc agcggacgcg cgtcctgacg
     6721 cggcccggca tgacgaaaga gaagttcgaa gcgcttattg cccgccaact cacggacgcg
     6781 gagcgccggg cgagggccga tctcgtcgtg gatagcggat cgacactgga gaacttgcac
     6841 acccaactcg atcgattgct cgaatcgctg caaactcggg acggtacggt catggagcag
     6901 ttgcgttcac aaaagtccta gccggaggtt ctaagcgttg cgcgagatca ttctcgatac
     6961 cgagaccaca gggcttgatg ctttcgacgg cgatcgcatc gtcgaaatcg gctgcgtgga
     7021 actcgtcaac agcatcgcca cgggccgcac ctggcattgc tacctgaacc ccgagcgcga
     7081 cgtgccggag caagccttca aggtgcacgg tctctcgacc gatttcctca gcgacaagcc
     7141 gttgttcgaa gagcgggccg acgcgttttt agaattcgtc gaggggtcga tgctcgtcat
     7201 ccacaacgct tccttcgact tcgggtttct caatgcggaa ctggaacgtg cgtcacgccc
     7261 tcttttgaat tgggatcggg tgatcgacac gctggccttg gcccggcggc ggcatcccgg
     7321 cgcgcccgca agcctcgatg cactgtgccg ccggtatggc atcgatctca gcgaacgcga
     7381 tttgcacggc gcacttctcg attgccgcct gctggcctct gtctatgtgg agctggtggg
     7441 cgggcaccag gcgcggctcg aatttgccac caacggcgca caagccgcga tccttcaggc
     7501 gcgggaatcg gcggtccgta cgcggccggc gccactcgct cctcgggtta gcgaggcgga
     7561 gcacgaagcg catagcgcgt tcgtcgcaac gctcggcgag gatgcgctgt ggctgcgcta
     7621 cgcctgatcg tcttcttctg agaggttagt tcggctgggc ctgcgcggct ttctgatccc
     7681 ctgccatgtt ctgcgcatac agcctggcga agtcgatcgg gtcgagcatc agcggcggga
     7741 agccgccatc gcggacgaca tcggacagaa cacgccgcac gaaggggaat aggatggtcg
     7801 ggcagtcgac gaacatgatc ggctgcaaca tgtcttgcgg aacgttgacg atccggaaca
     7861 cgccggcata gaccagctcg gcattgtaga tcacgcctac atcgcttttg gcgtgggcct
     7921 cgatattgac ggccacttcg tagatgtcct cgccgcgcgc acccgcattg acgttgacgc
     7981 caactttcag ctgcgggttc tcgcccgggc cttgaagggt ttgcggcgcg cccgggcttt
     8041 cgaacgacag gtccttgacg tattgggcga cgacgttgag ctgcggccgc gcattcgcgc
     8101 cctcgccggc cgtcgacggt ccgctgccgc cggctgttgc cggggcttcc tttggcgtcg
     8161 gtccatcgcc attggtcctc ttcgcgttgt cgtctttcgg cttcctggcc atggggctct
     8221 ccctcacgcg gctaatgcgt ctgttggcta gcacggcacg tttgggcccg caattcgtac
     8281 gctggcgctg ctagcgccca aattaagtgc cccggccttt agggttggct tcgcgtcagg
     8341 gaatgccgta agttgagcta agaatgctgc tgccgggcca acttggctgc ggcgaaggct
     8401 cccgctagaa aatcgatagg cgcgcaacga tattggtcct gacgcatttc gcatatgact
     8461 tgcgggtttc agcgagcgac gcgcgtggcg gcaaggcatg agtgaagttt tcgacatcta
     8521 tacattgctg ttcctggtgc tggcggtcgt gatcttcatc cggctgcgcg gcgtactagg
     8581 acggcgcacg ggcaatgaac gcccgcctta cgatccttac acgcgccccg atgccaagca
     8641 ggcggggacg cccgaaggca ccgtggtggc gctgccgcgc ggccgcacgg gcaaaccggc
     8701 cggcgagaac gccggaccga cgcaagaaga catcgacgaa cggctcgaac gctatgccac
     8761 taaggacagc ccgctcggca agtcgctgac ggcactgatc aaggtcgacc ccagcttcga
     8821 cccgggacag ttcttggatg gcgccaaggt tgcctacgag gtcatcgtgg gcgcgttcgc
     8881 cgacggcgat gagaagacgc tgaagcaatt gctcggcgac gacgtgctcg acggcttcat
     8941 gcgggcgatc gacgagcgcg aagggcgcgg cgaacaggcg cagacgacgc tggttggcat
     9001 cgacaaggcc gacattctgg aagccgagat caaggacaag caggctttcg tcacggtgaa
     9061 attcgtgagc gagctgatct ctgtcacgcg ggacggcgaa ggcgatgtcg ttgacggcga
     9121 tcccacgaaa gttcgcgagg tgaccgacat ctggacgttc gcccgtggca ttgactcgcg
     9181 caatcccaac tggaagctcg tcgccaccga agcggcgaac tagccggccg gtgacccggc
     9241 ggggcccggc ggcagacccg ttcgatcgcg actttccgcc gctctcattc gacatgctgg
     9301 aaggttggga ggacgacgat catctcgccg ccttccgtgc ctttctgaaa tcgtgcgagc
     9361 acgatgcttt gagcgatctc gagaaggcca gttttccccg cgaggaggcc ctggcgctcg
     9421 gccccgatat cggcaaagac gccgcgcgcg agttcttcga gacgcatttc acgccccggt
     9481 gccggtatga cagccggctc gatgacggct tcgtcaccgg ctattacgaa ccgatggtgc
     9541 gcggcgcacg cgaacggagc gcgcaatatt gcgttcccgt ctatggccgg cccgacgatc
     9601 tcgtcatgct ttccgatgat ctctaccgcg cggcggccaa tgacaggatc agcgcgcggc
     9661 gggactgcgg tgacgcgctc gaggattact tcacgcgcgc gcagatcgaa gacggcgctc
     9721 tgaaggatcg cgggttggag gcgctctatc tcgacgattg gatcgagacg ttcttcatgc
     9781 acgtgcaagg ctcagggctg gtgcagctcg atgacggcac tgaagtgcgc ctcgcttacg
     9841 acggcaagaa cggtcatccc tatacgtcca tcgcgcggca gctggtcgac cggcgcgaag
     9901 tgttgccgaa ggacatcgac atggagaagg tcaaggcctg gctgcgcgcc gacccggagc
     9961 ggggtcgcgc gctgatgcag gagaaccagt cctacatctt cttccgcgaa cttgagcgtg
    10021 acgagggacg cgacggtccc ttgggtgcgg aaggcgtgcc gctcaccgcg gggcggagtc
    10081 tcgccgtcga tccggtctac acgccgctag gctgtccggt ctatctgctt gccgccaatc
    10141 tgccgtccga aagaggcccg gagttcgccc ggctgatgat tgcgcaggac gtgggctcgg
    10201 caattcgcgg acctgagcgt ggcgatatct tctggggctc gggcgaggcc gccggggcga
    10261 tcgccggcac cacgcggcat ctggcggttt tcatcttcct gctgccgaac ccttaacgga
    10321 attcgcgcta tggtccgccc cgatgggcaa gcgcgacggc gaaaaatcag acatcgagga
    10381 cgacgctgac ttgt
//
LOCUS       DBNHVU010000088         9515 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1893378, whole genome shotgun sequence.
ACCESSION   DBNHVU010000088 DBNHVU010000000
VERSION     DBNHVU010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 9515)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9515)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9515
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1893378"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="AC16Y7_03320"
     CDS             complement(<1..1179)
                     /locus_tag="AC16Y7_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HIR5708443.1"
                     /translation="MAETNAAASVEAMSPWQNCRGALRVAVCLALALFLFLPGLAYAI
                     EAIPVEPDNPKVDITAKGELYEGRGDRLQIETAPGPDGYIGRMAVQASTPGTNPGWLV
                     FALSNPTDKRIVRWLVAPRYTLANSRVFRPELDAPRITAVTPSLGFRPEPLKSDRADM
                     FRLSLEPGQTVTYAAELASSKVPSLTLWTPNIYQAKQQDRMLFNGVLLGITGLLAIFL
                     TAIFAANHRAIFPATALVAWAALVYFCVDFGFWNKLFPVGPDRTALYRAAAEAGLFAS
                     FVLFLYTFLRIGLWHMWIKTVFWVWIAAQFALIVVAIVDPQTAAGLARASAFVIAGVG
                     TALIVYFALRGQDRAMSLIPSWMLLIVWILGAAMIVLGKLSGEVVVSGLIAGLVLIIV
                     L"
     gene            complement(1207..1794)
                     /locus_tag="AC16Y7_03325"
     CDS             complement(1207..1794)
                     /locus_tag="AC16Y7_03325"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5708444.1"
                     /translation="MALLRAASPSDFGPAIEAGRVMLRTPQMSDYPAWAELRATSRDF
                     LMPWEPLWAPDELSRASFRRRVRHYLRDLREDVGYALFIYDKVTNALVGGLTLCNVRR
                     GVTQSCTLGYWVGAEYAQQGYMTAAVRAVVPFVFDTLELHRLEAACLPTNTASMRLLD
                     KTGFQREGLARRYLRINGVWQDHILYALLDSDPRI"
     gene            complement(1810..3090)
                     /locus_tag="AC16Y7_03330"
     CDS             complement(1810..3090)
                     /locus_tag="AC16Y7_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR5708445.1"
                     /translation="MSVERSELANGLTVVSHAMPEVETVSLGIWIGAGSRSEAHAEHG
                     VAHFLEHMAFKGTARRSARDIVEEIEAVGGDLNAATSVDSTAYYARVMRGDMSLALDI
                     LSDIILEPRFDMAELRRERDVILQEIAASLDSPEDIAYDFISEAAFPNQAVGRPILGT
                     VQSVAGFSRDHLGSYLSAHYHAPNMVLAAAGAVDHEALVTEAERRLAGLDRNASPKPE
                     TAVYRGGVKRSDKPFEQTHLMLAFEAPPYRHDDYFTAQILAGTLGGGMSSRLFQEVRE
                     RRGLCYSVYAFSSGLADSGMFVVHAAGSPEKADDLFGVIRGELARAADDGFDDAEIAR
                     VKAQIKMGLLAGLESSGARAEQIARQILVHGRVLTTDEIIEEVERVETADIQSMVARL
                     LGSPVSLATVGPILNVAKFESVAEKFRLPASRAA"
     gene            complement(3087..4517)
                     /gene="thrC"
                     /locus_tag="AC16Y7_03335"
     CDS             complement(3087..4517)
                     /gene="thrC"
                     /locus_tag="AC16Y7_03335"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR5708446.1"
                     /translation="MRQTLKFISTRGQAPALPFEGALMAALASDGGLFMPEVWPQLSA
                     DEIEGLAGLDYAKAAYRVMSPYLASDACLQDLEEVLSDAYDGFHHPAVVPLTQLGPDS
                     FVLELFHGPTLSFKDLAMQVVARFMNRVLKRQGARATVIGATSGDTGAAAIEAFRGLD
                     AIDIFILHPKGRISDVQRRQMTTALEPNVHNIAIEGTFDDCQAIVKAMFGDPELRGRL
                     HLTGVNSINFARILAQIPYYFTSAVSLGAPHQAVAFTVPTGNFGDIFAGYAARAMGLP
                     VQRLVIATNLNDSLPRALASGAFEPQGVVATSSPAMDIQLASNFERLLFELAGRDAAR
                     IRALMEELRTTGAFRLSQGELGQLRELFGAHAVDEQETQMTIRGVFEETGMLVDPHTA
                     VGITASAREDVAPTPMVVLSTAHPAKFPDAVKRATGRVPEMPERLKLRLGREERCASL
                     PPEFGAVSEFIVSHGRDIPQQAEVDA"
     gene            complement(4594..5322)
                     /locus_tag="AC16Y7_03340"
     CDS             complement(4594..5322)
                     /locus_tag="AC16Y7_03340"
                     /inference="COORDINATES: protein motif:HMM:NF014193.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial respiratory chain
                     complex IV assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HIR5708447.1"
                     /translation="MQTGSLLKLTIATLIGVSLLVGLGVWQLQRLEWKEGIIARIEAR
                     TEREPMSLERALDIVRRYPNPDYFPVRVEGRFHHDRERYLYALSLEGEPGWHVITPLE
                     TVGGEVVLVDRGFVPEAMRDPVTRTQGQVEEVGAVTGLIRSPETPGIFIPENEPDRNQ
                     WFSRDLTAMGRSMFPGGTVEPIPFFLEARKSDVPGGLPLGGQTRLTLTNNHLQYALTW
                     FCLAVVLVVVYAAYMWGALRGPRP"
     gene            complement(5330..5716)
                     /locus_tag="AC16Y7_03345"
     CDS             complement(5330..5716)
                     /locus_tag="AC16Y7_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HIR5708448.1"
                     /translation="MTNVHDPNVPLGATIRSGLTGRCPSCHKGKMFAGYLTLAPRCET
                     CELDYGFADAGDGPAVFVILITGFIVVGLALIAEMLYQPSYWLHALVWGSLAIVLPLL
                     LLRCFKGVLVALQFRHKAEEGKLLSE"
     gene            complement(5787..6674)
                     /locus_tag="AC16Y7_03350"
     CDS             complement(5787..6674)
                     /locus_tag="AC16Y7_03350"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR5708449.1"
                     /translation="MAQAHAKPQHDYHLVNPSPWPIIGSVGALTLAIGVLMYFMSKKA
                     GDPQLWYVLPGLALVVLTMFGWWRDVIKEAHGGDETPVVQLHLRYGMILFIASEVMFF
                     VAWFWAYFDVALFPASDVTYARTEVTGGQWPPIPSDETGVAGLGYFGHTFSPWGLPWV
                     NTLILLLSGTTATWAHHAMLHNDRKNLVWALAITVVLGALFTCLQAYEYMHAGFGFSN
                     HIYGSTFYMATGFHGFHVIVGTIFLLVCLFRALQGHFTPKQHFGFEAAAWYWHFVDVV
                     WLFLFTCIYVWGAGANAGH"
     gene            complement(6734..7348)
                     /locus_tag="AC16Y7_03355"
     CDS             complement(6734..7348)
                     /locus_tag="AC16Y7_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HIR5708450.1"
                     /translation="MTDGNEKDLKPAPAPKRDRKGLVALSLVGLVAGMVGLSFAAVPL
                     YRMFCQVTGYGGVPQIAEALPGEVLDRTITVRFDANVDRALPWDFTPEQRAIDVRIGE
                     SGLAFFKAHNNTDKPVTGTAGFNVVPEIAGRYFNKIECFCFKEQTLVAGQSIDMPVTF
                     FVDPKIVEDMSTKNISEITLSYTFYRSDDPSDVAATSRDGASGS"
     gene            complement(7361..7504)
                     /locus_tag="AC16Y7_03360"
     CDS             complement(7361..7504)
                     /locus_tag="AC16Y7_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708451.1"
                     /translation="MEDAEAERKRRQRKRSLAIAWILAGLAILFFIVTIVRLGGNVAV
                     RQM"
     gene            complement(7504..8460)
                     /locus_tag="AC16Y7_03365"
     CDS             complement(7504..8460)
                     /locus_tag="AC16Y7_03365"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR5708452.1"
                     /translation="MSDVTVQAIPGKLDVAPGGDVSDYAALLKPRVMFLVVFTALVGL
                     VAAPGSMHPILAIAALACIAVGAGASGALNMWYDADIDARMARTAGRPLPQGRITPGE
                     ALAFSSVLSIGSVLCLGVMANWVAAALLAGTIAFYVLVYTMWLKRSTPQNIVIGGAAG
                     AVPPMIGWAAATGTVSLESFVMFLIIFIWTPPHFWALALVRSKDYERAGVPMLPVTHG
                     PDETRRQILIYSLILAPLGMAPSVMGFGGMLYTVTASILGAFFVVFAFDCYRIRKGEE
                     ADRAAKHLFAYSVLYLFVLFAVLLVEQGFGFESGAASLSWTL"
     gene            complement(8495..>9515)
                     /locus_tag="AC16Y7_03370"
     CDS             complement(8495..>9515)
                     /locus_tag="AC16Y7_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR5708453.1"
                     /translation="GMTLHKMPLFVWAVLVTAFLLLLSLPVLAGAITMLLTDRNFGTA
                     FFDSAHGGDPLLWQHLFWFFGHPEVYILIIPGFGIVSQIVSTFSRKPVFGYLGMAYAM
                     VAIGLIGFVVWAHHMYAAGLDVNTQAYFVFATMVIAVPTGVKIFSWIATMWGGSIRFD
                     TPMLWAIGFIFLFTVGGVTGVVLSNAGVDRSYHDTYYVVAHFHYTMSIGAVFSIFAGW
                     YYWAPKMFGYMYSEAVGKLHFWLMFIGANLTFFPQHFLGAAGMPRRYADYPDAYAGWN
                     LISSIGAYIGFAGLLVFLFSIVYMFVRKQKAAANPWGPGATTLEWTLSSPPPFHTFDT
                     LPRIK"
BASE COUNT         1991 a         2939 c         3050 g         1535 t
ORIGIN      
        1 gagcacgatg atcagcacga ggccggcaat gagacccgag acgacgacct ctcccgacaa
       61 cttgccgagc acgatcatgg ctgcgccgag gatccacacg atgaggagca tccaactcgg
      121 gatcagcgac atggcgcgat cttgtccgcg cagcgcgaag tacacgatga gcgcggtgcc
      181 gacaccggct atgacgaagg cggaagcgcg ggcgaggccg gcggcggttt gcggatcgac
      241 gatggcgaca acgatcagcg cgaactgagc ggcgatccag acccagaaca cggtcttgat
      301 ccacatatgc cacaggccga tacgcaggaa ggtgtaaagg aacagcacga agctcgcgaa
      361 aagtccggct tcggcggcgg cccgatagag cgcggtgcgg tcggggccta cggggaacag
      421 cttgttccag aaaccgaagt cgacgcagaa atagacgagc gcggcccagg cgacgagcgc
      481 ggttgcggga aagatcgccc ggtggttggc ggcaaagatc gcggtgagga agatggcgag
      541 caggccggtg ataccgagca ggaccccgtt gaacagcatg cggtcttgct gtttcgcctg
      601 gtagatgttc ggcgtccaca gggtgaggct cggcactttc gatgaggcga gctcagcggc
      661 gtatgtcacc gtctgtccgg gctccaggct caagcggaac atgtccgcgc ggtcgctttt
      721 cagtggttcg ggacgaaagc cgagagacgg cgtcaccgcg gtgatgcgcg gcgcatcgag
      781 ctcggggcgg aagacgcggg agttagccag cgtatagcgc ggcgccacga gccagcgcac
      841 gatgcgcttg tcagtcgggt tcgacagggc gaagacgagc caacccggat tggtgccggg
      901 cgtggaggcc tgcaccgcca tgcggccgat atagccgtcg ggaccgggcg cggtctcaat
      961 ttgaagtcga tcgccccttc cctcgtagag ttcgcccttg gcggtaatgt cgaccttagg
     1021 attatccggt tcgacgggga tcgcctcgat ggcgtaggcc agcccaggca gaaaaaggaa
     1081 caacgcgagc gcaaggcaca ccgccacgcg aagcgcgccc cggcagtttt gccagggaga
     1141 catcgcttca acgcttgcag cggcgtttgt ctccgccatt actcccccgt tcatcaccgc
     1201 atcccgctaa atacgcggat cggaatccag cagcgcatag agaatgtggt cctgccagac
     1261 gccgttgatc cgcagataac ggcgcgccag gccctcgcgc tggaaaccgg tcttgtcgag
     1321 aagccgcatg gaagccgtgt tggtcggcaa gcacgcggct tccagcctat gcagttcgag
     1381 cgtgtcgaat acgaaaggca cgacggcgcg tacggccgcc gtcatgtaac cctgttgggc
     1441 gtactccgcg ccaacccaat agccgagcgt gcaggactgg gtcacgccgc gccggacgtt
     1501 gcaaagcgtg aggccgccga cgagtgcgtt ggtcaccttg tcatagatga agagcgcata
     1561 gccgacatcc tcgcgcaggt cgcgcaagta gtgccgcacg cggcggcgga aggaagctcg
     1621 cgacagctcg tccggcgccc acagaggctc ccaaggcatt agaaagtcgc ggctggtcgc
     1681 tctgagctcg gcccaagcgg gataatcgga catctgcggg gtccgcagca tgacgcgccc
     1741 agcctcgatg gccgggccaa aatcgcttgg ggatgcagct cgcagcaatg ccatcgctta
     1801 gcaccctctt caggcagctc tgctcgccgg gagcctgaac ttttcggcca ccgattcgaa
     1861 ttttgcaaca ttaagaatgg ggcccacggt ggcaaggctc acgggcgatc ccagcaaacg
     1921 cgcaaccatg gattgaatat cggcggtctc caccctctcg acctcttcga tgatctcgtc
     1981 ggtggtcagc acccggccgt gaacgaggat ctgccgcgca atctgctcgg cgcgggcgcc
     2041 ggagctttcc agtccggcca agagccccat cttgatctgc gccttgaccc gggcaatctc
     2101 cgcgtcgtca aagccgtcgt cggccgcgcg cgccaactcg ccccgaatga cgccgaacag
     2161 gtcgtcggcc ttttcgggac tgcccgccgc gtgaacgaca aacatgccgc tatcggcgag
     2221 gcccgaggag aaggcataga cggaatagca aaggccgcga cgctcccgca cctcttggaa
     2281 caggcgcgac gacatcccgc ccccgagcgt gccggcaaga atctgcgccg tgaagtaatc
     2341 gtcatggcgg tagggcggcg cctcgaaggc caacatgagg tgcgtctgct cgaacggttt
     2401 gtcggaccgt ttgacgccgc cgcggtagac ggccgtctca ggctttggcg aggcgttcct
     2461 gtcgaggccc gcaagccggc gctccgcctc ggtcaccagc gcttcgtggt ccacggcgcc
     2521 cgccgcggcg agcaccatgt tgggcgcatg atagtgggcg gataaatacg acccgagatg
     2581 atcgcggctg aacccggcaa cactctgcac cgttccgagg atcgggcggc cgacggcttg
     2641 gttgggaaac gccgcctccg aaataaagtc gtaggcgatg tcctccggcg agtccaaaga
     2701 ggcggcgatc tcctggagga tcacgtcgcg ctcgcgccgg agctcggcca tgtcgaaccg
     2761 gggctcaagg atgatgtcgc tcaagatgtc gagggcgagc gacatgtcgc cccgcatcac
     2821 cctggcgtaa taggcggtgg aatccacgct ggtggcagca ttgagatcgc cgccgaccgc
     2881 ttcgatctcc tcgacgatgt cgcgggcgct gcgccgcgcg gtgcctttga aggccatgtg
     2941 ctcaagaaaa tgggcaacgc cgtgctcggc atgagcctcc gaccggctgc cggcgccgat
     3001 ccagatgcca agggacacgg tctccacctc gggcatggcg tggctgacga ccgtcaagcc
     3061 attggcgagc tctgaacgtt cgacgctcac gcgtccacct ccgcctgctg tggtatgtcg
     3121 cggccgtggc tgacgatgaa ctcagagacg gcgccgaact cagggggaag cgacgcgcag
     3181 cgttcttctc gaccgagacg cagctttaag cgttccggca tctcggggac gcggccggtc
     3241 gcgcgcttca ccgcgtccgg gaacttcgcc ggatgcgccg tggacaggac gaccatgggt
     3301 gtgggggcca cgtcttcacg cgccgatgcg gtgatgccga ccgccgtatg cggatcgacc
     3361 agcatgcccg tttcctcgaa aaccccccga atggtcatct gcgtctcttg ctcgtcaacg
     3421 gcgtgcgcgc caaacagctc gcgtagctgg ccaagctcgc cttggctcaa tcggaacgca
     3481 ccggtcgtgc gcaactcttc catcagcgcc ctgatgcggg cggcgtcgcg accagcgagc
     3541 tcgaatagaa gccgctcgaa attgcttgcc agctggatgt ccatggccgg actagacgtg
     3601 gcgaccacgc cttgcggctc gaaggccccc gacgcaagcg cgcgcggcaa actgtcgttg
     3661 aggttcgtgg cgataacgag gcgctggacc ggtaagccca tggcgcgggc cgcgtaacct
     3721 gcgaaaatat caccgaaatt tccggtgggc acggtgaagg ccacggcctg atgcggcgcg
     3781 ccgaggctca cagccgaggt gaagtagtat gggatctgcg cgaggatccg ggcgaagttg
     3841 atggagttca cgcccgtcag atgaagccgg ccgcgaagct ccggatcgcc gaacatcgcc
     3901 ttcacgatcg cctggcagtc gtcaaacgtg ccttcaatgg cgatgttgtg gacgttgggc
     3961 tcaagggccg tggtcatctg ccggcgctgg acatccgaga tgcggccctt ggggtgcagg
     4021 atgaagatgt cgatggcgtc gagcccccgg aaggcttcga tggccgccgc gcctgtgtcg
     4081 cccgaggtgg cgccgatcac ggtggcgcgg gcaccctggc gcttcaggac ccggttcatg
     4141 aaccgggcca cgacctgcat ggcgagatcc ttgaaggaga gcgtgggccc atggaacagc
     4201 tccaggacga agctgtccgg gccaagctgc gtcagcggaa cgaccgccgg atggtggaag
     4261 ccgtcatagg cgtccgagag cacctcttcc aagtcctgaa ggcacgcatc gctggccaga
     4321 taggggctca tcacccggta ggcggccttg gcatagtcga gcccggcaag gccttcgatc
     4381 tcgtccgcac tgagctgcgg ccagacctcg ggcatgaaca gcccgccatc ggaagccaga
     4441 gcggccatga gcgcaccctc aaaggggagc gccggcgcct gcccacgcgt cgagatgaac
     4501 ttcaaggtct gcctcacgtc gaaaaaaggg ggcttttcgg cctgacccta gcggccggag
     4561 aggccccaag caacatgacc aagcttggct cagttaaggc cgcgggccac gcagagcccc
     4621 ccacatatag gcggcataga ccaccaccag gaccactgcc aggcagaacc aggtcagagc
     4681 atattgcagg tgattattgg ttagggtgag ccgggtctgc ccgccgaggg gaaggccgcc
     4741 cgggacgtcg ctctttcttg cctccagaaa aaagggaatc ggctcaacgg tgccgcccgg
     4801 aaacatggag cggcccatgg cggtgagatc ccggctgaac cactgattcc tgtcaggctc
     4861 gttctcggga atgaagatgc cgggtgtctc ggggctgcga atgaggcccg tgaccgcccc
     4921 cacctcttcg acttggcctt gggtccgcgt gacggggtcg cgcatggcct cgggcacgaa
     4981 gccgcgatcg accagcacca cctccccgcc cacggtctcc aagggcgtaa tgacgtgcca
     5041 gccaggttcg ccttcgagag acaacgcata gagatagcgc tcccggtcgt gatggaagcg
     5101 cccctcgacg cgcacgggga aatagtccgg gttcgggtac ctgcggacaa tatcgagggc
     5161 ccgctccagc gacatgggct cgcgttcggt tcgcgcttcg atcctggcga tgatgccttc
     5221 cttccactcc aagcgttgga gttgccatac cccaaggccg acaagcaggg acacgccaat
     5281 aagcgtagcg atcgtcagct tcaggaggct gccggtttgc atcgcgcgcc tactcggata
     5341 gcagcttgcc ttcttctgcc ttgtgtcgga actggagcgc gacaagcacg cccttgaagc
     5401 accgaagcag aagcaaaggc aagacgatgg cgaggctgcc ccacacgagc gcgtgcaacc
     5461 aataagatgg ctggtagagc atctcggcga tgagcgcgag gccgaccacg atgaagccgg
     5521 tgatgagaat gacgaagacg gcaggcccat cgcccgcatc ggcaaagccg tagtcaagct
     5581 cgcaggtctc gcagcgcggt gcgagcgtga gatagccggc gaacatcttg cccttgtggc
     5641 aggacggaca gcgccccgtc aggccggacc ggatggtcgc gccgaggggg acgtttggat
     5701 cgtgaacgtt ggtcatcttt actcaggccg aaataaaaag ggggacggtc ctacgaccgc
     5761 cccccagaat agacgattgt tcgcccctag tgtccggcgt ttgcgcccgc gccccacaca
     5821 tagatgcagg tgaacaggaa caaccatacg acgtcgacga aatgccagta ccaggcggcc
     5881 gcctcaaagc cgaaatgctg cttcggcgtg aagtggcctt gcaacgcccg gaacaggcac
     5941 accagtagga agatcgtgcc gacgatcaca tggaagccgt ggaagccggt cgccatgtag
     6001 aaggtcgagc cgtatatgtg gttcgagaac ccgaacccgg catgcatgta ctcgtaggct
     6061 tgcaagcagg tgaagagcgc accgagcacg accgtgatcg cgagcgccca gaccaaattc
     6121 ttacggtcgt tgtgcagcat cgcgtggtgt gcccaggtcg ccgtcgtgcc ggagagcagc
     6181 aggatcagcg tgttgaccca gggcaagccc cacgggctga aagtgtgccc gaaatagccg
     6241 agaccggcga cgcccgtctc gtcgctcggg atgggcggcc actgaccgcc cgttacttcc
     6301 gtgcgagcgt aggtgacatc actggcgggg aacagcgcca cgtcaaaata ggcccagaac
     6361 caggcgacga agaacatcac ctcagaggcg atgaacagga tcatgccgta gcgcaggtga
     6421 agctgcacga ccggcgtctc gtcgccgcca tgcgcttcct tgatgacatc gcgccaccag
     6481 ccgaacatgg tgagaacaac cagcgccagg cccggcagga cataccaaag ctgcggatcg
     6541 cctgccttct tcgacatgaa gtacatcaag acgccaatcg ccagcgtcag tgcgccgacc
     6601 gaaccgatga tcggccacgg acttggattg acgagatggt aatcgtgttg cggtttcgcg
     6661 tgggcctggg ccatgtttgg ttcccctctt ctccccagtc tatctcattg acgcctttgc
     6721 cgcgccattc agcttacgat cccgaggccc cgtcgcgcga cgtagcagcg acatccgacg
     6781 ggtcgtcgct ccggtagaac gtataggaca gcgtgatctc ggaaatattc tttgtgctca
     6841 tatcctcgac gatcttggga tcgacgaaga aggtcaccgg catgtcgatg ctttgacctg
     6901 cgacgagcgt ctgctccttg aagcagaaac attcgatctt gttgaagtag cggccggcga
     6961 tctcgggcac cacgttgaat cccgcggtgc cggttaccgg cttatcggtg ttgttgtgcg
     7021 ccttgaagaa ggctagcccg ctctcgccga tgcgtacgtc gatagcgcgc tgctcgggtg
     7081 tgaagtccca ggggagcgcg cgatccacat tggcgtcgaa ccgaacagtg atggtgcggt
     7141 cgagaacctc gccgggcagc gcctcggcaa tctgtgggac accgccatag ccggtgacct
     7201 ggcagaacat gcggtagagc ggcactgcgg cgaaggacaa gcccaccatg ccggcgacaa
     7261 ggcccaccag cgacaacgcg accaagccct tccggtcgcg ctttggcgcc ggtgcgggct
     7321 tcaagtcttt ttcgttgccg tccgtcatgc gtcccgccaa ttacatctgc cgcacggcga
     7381 cattgccgcc gagccgcaca atggtaacga tgaagaacag aatcgcgagg cctgcgagga
     7441 tccaggcaat ggccaaagat cgcttgcgtt gacggcgttt gcgttcggct tccgcatctt
     7501 ccatcacaag gtccagctca gcgacgctgc gccgctctcg aatccgaagc cttgctccac
     7561 caggagcacg gcaaagagca cgaaaagata aagtaccgag taggcgaaca agtgcttggc
     7621 ggcccggtcg gcttcctcgc ccttgcgtat gcgataacaa tcgaacgcga agacgacaaa
     7681 gaacgcgccc aggatcgagg cggtgacggt gtaaagcatg ccgccaaagc ccatgactga
     7741 cggggccatg cccaacggcg cgaggataag gctgtagatc aggatctgac ggcgggtctc
     7801 gtcaggcccg tgcgtcaccg gcagcatagg cacgccagcg cgctcatagt ctttgctgcg
     7861 gaccagagcc agcgcccaga agtgaggcgg cgtccaaatg aagatgatga ggaacatcac
     7921 gaaggactcg aggctcaccg taccggtggc cgcggcccag ccgatcattg gcggcacggc
     7981 gccggcagcg ccccctatca cgatgttctg cggcgtcgag cgtttcagcc acatcgtgta
     8041 gacgagcacg tagaaggcga tggtgcctgc gagcaaggct gctgcgaccc agttcgccat
     8101 cacgccaagg cacaacaccg agccgatcga gagcacgctg ctgaaggcca gcgcttcgcc
     8161 cggcgtgatc ctgccttgcg gcaaaggacg cccagcagtg cgggccatgc gcgcatcgat
     8221 gtcggcgtcg taccacatgt tcagcgcgcc agaggcgccc gcgccgacag cgatgcaggc
     8281 gagcgcggcg atggctaata tcgggtgcat gcttccgggc gcggcgacta ggccgaccag
     8341 cgccgtgaac accacaagaa acatgacccg cggcttgagc agcgcagcat aatcggagac
     8401 gtcgcccccg ggggcgacgt cgagctttcc tggaatcgct tggacagtga cgtcggacat
     8461 cagctactca ctcaagcgcc ctccaaccat cgacctactt gatacgcggc agcgtatcga
     8521 aggtatggaa cggcggcggg gacgacagcg tccactcaag cgtcgtggca ccgggtcccc
     8581 aaggattggc cgcagccttc tgcttcctca cgaacatgta gacgatcgag aacaagaaga
     8641 ccaaaagacc ggcgaagccg atataggcgc cgatggacga gatcaggttc cagcccgcgt
     8701 aagcatccgg atagtctgca tagcggcgag gcatgccggc ggcaccgagg aagtgctgcg
     8761 ggaagaaggt cagattggcg ccgatgaaca tcagccagaa gtgaagttta ccgacggcct
     8821 cggagtacat gtagccgaac atcttcggcg cccagtagta ccagccggcg aagatcgaga
     8881 acacggcgcc gatggacatc gtgtagtgga agtgcgccac cacgtaatag gtgtcgtgat
     8941 aggagcgatc cacaccagcg tttgacagca ccacaccggt tacaccacca acggtgaaca
     9001 ggaagatgaa gccgatggcc cataacatcg gcgtgtcgaa cctaatggag ccgccccaca
     9061 tggtggcgat ccacgagaag atcttcacac ccgtgggcac agcgatgacc atcgtggcga
     9121 acacaaaata agcttgcgtg ttcacatcga gaccagcggc gtacatgtgg tgggcccata
     9181 cgacgaagcc gatcaagccg atcgccacca tggcataggc cataccgaga tagccaaaca
     9241 caggcttcct cgagaacgtc gacacgatct ggctgacgat gccgaagccc ggaatgatga
     9301 gaatgtacac ttccggatgg ccgaagaacc agaacaggtg ctgccacagc aacggatcgc
     9361 caccatgcgc ggagtcgaag aaggcagtgc cgaagttgcg gtcggtcagc aacatggtga
     9421 tggctccggc cagcacgggc agcgacagca acaacaggaa ggccgtcacc agcacggccc
     9481 atacgaagag cggcatcttg tgcaaggtca tgccc
//
LOCUS       DBNHVU010000089         6099 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_703640, whole genome shotgun sequence.
ACCESSION   DBNHVU010000089 DBNHVU010000000
VERSION     DBNHVU010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_703640"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="AC16Y7_03375"
     CDS             complement(<1..239)
                     /locus_tag="AC16Y7_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708454.1"
                     /translation="MTLQYVQITETRRVPIERPVLKLIVTALLFTAAVAVAVPSAAEE
                     CPYTPARDSAERKAILDAVRKPVSKELGQRVIFVVS"
     gene            complement(292..2025)
                     /locus_tag="AC16Y7_03380"
     CDS             complement(292..2025)
                     /locus_tag="AC16Y7_03380"
                     /inference="COORDINATES: protein motif:HMM:NF042645.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HIR5708455.1"
                     /translation="MRFLRVLRRTWLAGLLALFAFAPGFSLPGTARAGGPAVGYDDFA
                     IEQFYDELAPHGEWVYHPVHGYVWLARAVGPDWRPFTVGNWIYTNEYGWYWDSYEPFA
                     WAVYHYGRWGFDPAYNWYWVPGDTWSPAWVQWRYGDEYVGWAPEPPRAYGGGAYGAPV
                     HYSPVPPERSWVFVLPRYLTSPVVRSYAVPRTSISIALSFSKHVYRPEYRGGHVYNYG
                     MPRKRWSRLTRQRIEPRKIHRGRSKARPRAGRGRSSGDLYVYAPRVKKGARPKRPPRN
                     VNQARTRANANTGRIQTNPARARGARPNPNKPRALGSPAAGKPNIAGPRPRARAPQAR
                     PGGKRPYNANAPMPRPNKRATAKPGGPKTGNGAKAKRGPKKPSNANASKRKPNAKAAN
                     VKPRPKNGNGAKARSGQNKSGPKKPLNANASKHKPNAKAENVHPGRKNGNGPKAKSGQ
                     KKPGAKAPASKPKPKPASAKPKPKPKAASAKPKPKPKAASAKPKPKPKSAAKPKPKPK
                     AAAKPKPKPKAAAKPKSKPKPKAAAKPKPKAAAKPRPKPKPKAASAKPSPKPKPKPKS
                     AKSRKGGGKRG"
     gene            2311..3738
                     /locus_tag="AC16Y7_03385"
     CDS             2311..3738
                     /locus_tag="AC16Y7_03385"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HIR5708456.1"
                     /translation="MGRIYPVLLSGGTGSRLWPVSRAQMPKQLMPLLSGSSLLQETAN
                     RLAGLDDVAPPLVIANSDHRFMIAAQLQELGITATALVLEPVGRNTAAAAAVAAKIVG
                     DLDPESILVLLPADHHIPDAEGFKQAVRQGAELAARGHIVTFGITPRSPETGYGYIKR
                     GTALGDDAFTVAQFCEKPDLARADAFLADGSYDWNSGIFMARADTLTEEMKVHCPDIA
                     AQCTSAVAGSNRDMDFLRLDEPAFEACPSAPIDTAVMEHTSKAVVIPVDIGWSDVGSW
                     GALWDLGEKDEAGNVISGDVVALDTEETYIRGNGRLVTTLGVKDLIVVDTGDAVLIAD
                     KARVQDIRDVVTRLKELGRSEHETHSRVHRPWGYYESIEAGDRYQVKHLMVKPGASLS
                     LQMHHHRAEHWVVVKGTARVVLGNKEMLLSENESTYIPIGTPHRLENPGKVPLSIIEV
                     QSGCYLGEDDIVRMDDVYGRLAETP"
     gene            complement(3936..5882)
                     /locus_tag="AC16Y7_03390"
     CDS             complement(3936..5882)
                     /locus_tag="AC16Y7_03390"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR5708457.1"
                     /translation="MAPPRLRYRAEIDGLRALAVFLVVLFHAGFWGAPGGFVGVDVFF
                     VISGYLITSLLLREFEERGTIRLMDFWARRMRRILPAGVLVLCASLVGAAIFLPPALE
                     SRAAKELPAAALYFINWRLADKAVDYFADNTEPSLFLQYWSLAIEEQFYAVWPLIVIL
                     ISIVAVRFFRNTALARVLMWTAIAITLMSFGTCLYLTGHNQPYAFFGTFSRAWQLGIG
                     AVIGLLPLVSIDVYARRWMALAGIVAIVFAAVLFTNETPYPGLAAVLPTAGAAALILA
                     GRREDIGGLVGRLLKLPAVVLVGRWSYSWYLWHWPVLVLGTAYSTATPWLVFLVLLSL
                     LLAGLTYYVIEQPARYWPTLTRSPGISVTAGLLLSLGAAGFAVLAQATFAKPMIYLSN
                     GKRLNAEAIRKDKADECLLSYDETRQPPCIYGDASGSRRAVLFGDSHAASWLPAADLA
                     AKKAGWSLLARTKASCSSINAPLSNDRLSRPYVECSSWRDDVLSELKRLDPQLILLAN
                     NSSAPVIDATGERLSGEELENALIEAEKSLVDQLLADTNATIVLIRDITTSPKDPIIC
                     LLENPLNEDACRWNRKQTAYPRGTYEEEQRVKVVDLNNVICPSGTCRAILDGRVVMRD
                     SHHLTASFAATLASDFEPLFRLGP"
BASE COUNT         1182 a         1843 c         1868 g         1206 t
ORIGIN      
        1 gagaccacga agataacgcg ctgaccgagc tccttgctca ctggcttgcg gaccgcgtcc
       61 aggattgcct ttcgctcagc gctatcgcgc gcaggcgtat aaggacactc ttcggccgcg
      121 gacggcacag caaccgcgac ggcggcggta aagagcaagg ccgtcacgat cagcttcagc
      181 acaggcctct cgatcggcac gcgcctcgtt tcggtgatct gtacatattg tagcgtcatc
      241 tcaccttgac gaaactcaaa gcgcgaaacg catgccggac gaccgatccg tctagcctcg
      301 cttgccgccg cccttccgcg atttggcgga tttcggttta ggtttgggct tcggactagg
      361 ctttgcgctg gcggcttttg gcttgggttt cggcctcggc ttcgcggcgg ccttcggctt
      421 tggcttcgcg gcagccttgg gcttcggctt agacttcggc ttcgcggcag ccttgggctt
      481 aggcttcggc ttcgcagcgg ccttcggctt gggcttcggc ttcgcagcgg acttaggctt
      541 cggcttgggc ttcgcgctgg cggcctttgg cttgggcttc ggcttcgcgc tggcggcctt
      601 tggcttgggc ttcggtttcg cgctggcagg ctttggctta ggcttagatg ccggggcctt
      661 cgccccgggc ttcttctgtc cggacttggc cttgggaccg ttgccgttct tgcgcccagg
      721 atgcacgttt tccgccttcg cattgggctt atgcttcgag gcattcgcgt tcaaaggctt
      781 cttcggcccg gacttgttct ggcccgacct ggccttggcg ccattgccgt tcttcggccg
      841 aggcttcacg ttcgccgcct tcgcattggg cttacgcttc gaggcattcg cgttcgacgg
      901 cttcttcggc ccccgcttgg ccttggcgcc attgccagtc ttagggcctc ccggtttcgc
      961 agtggcccgc ttgttgggcc ttggcatcgg cgcgtttgcg ttgtacggcc tcttccctcc
     1021 cggcctcgcc tgaggtgccc tcgctcgcgg acgcggacct gcaatgtttg gtttgcccgc
     1081 ggcgggactc cccaaagcgc gcggcttatt cggattgggc cgagcgcccc tggcgcgtgc
     1141 gggattcgtt tgaatccttc cggtgttcgc gttcgcgcgc gttcgcgcct ggttgacatt
     1201 tctcgggggc cgcttcggcc gtgcaccttt cttgacgcgc ggcgcataca catagagatc
     1261 cccggatgaa cggccgcggc ctgcacgcgg acgtgccttg gaccggccgc gatggatctt
     1321 acgcggctcg atgcgctgtc gcgtaagcct cgaccagcgc ttgcggggca tgccgtaatt
     1381 gtagacgtgg ccgccgcgat actcagggcg gtacacatgc ttcgaaaagc tcagcgctat
     1441 cgagatgctg gttcgcggca ccgcgtaaga acgaacgacc ggcgatgtca gatagcgcgg
     1501 caggacgaag acccaagacc gctcgggcgg aaccggtgag tagtggaccg gcgcgccgta
     1561 ggcgcctcca ccgtaggcgc gcggcggctc gggcgcccag cccacatatt cgtcaccgta
     1621 acgccactgg acccaagccg gcgaccaggt atcgcccggg acccagtacc agttataggc
     1681 ggggtcgaag ccccaacggc catagtgata cacggcccag gcgaagggct cgtaggagtc
     1741 ccagtaccag ccgtattcgt tggtgtagat ccaattcccg accgtgaacg gacgccaatc
     1801 cggccccacg gcgcgcgcga gccatacata accatgtacg gggtggtaga cccactcccc
     1861 gtgaggcgcg agctcgtcat agaactgctc gatggcgaag tcgtcatagc cgaccgccgg
     1921 gccgccggcc ctggctgtcc caggaaggga aaaccctggt gcgaaggcga acagcgccag
     1981 cagacccgcc agccacgtac gcctcaagac gcgtagaaaa cgcatcggaa cctccgaatc
     2041 tcgcgcctgc cggcgctgaa ttaaccaagt cacctccttt cctcggcaga agcgccattc
     2101 gatccttttc aggcggaacg gcgatcatgg tggccagcat ggggttcggc acctgaaccc
     2161 aggctgaacg taaaacccca ctgatcccgg ggcctgcggg cggtattaat ctttcagaag
     2221 ttggcacgct ccatgcgtag gttgacggga ggccagttcc gtggtggacc ggacatggca
     2281 ccgtcagatc aacagtggtg tgttcgcgtc atgggccgca tttatccagt actgctttcc
     2341 ggggggacgg gctcccgact ttggcctgtt tcccgcgcgc agatgcccaa acagctgatg
     2401 ccgcttctgt cgggcagctc gctgctccaa gagaccgcta atcggcttgc cggcttggac
     2461 gacgtcgcgc caccgctggt gatcgccaac tccgatcacc gcttcatgat cgccgcccag
     2521 ctgcaagagc tcggcatcac ggccaccgca cttgtgctgg aacctgtggg ccgcaacacg
     2581 gcggcggccg cggccgtggc tgcgaagatc gtcggcgatc tcgatccgga gagcattctc
     2641 gttctactcc ccgccgatca tcacattccc gacgccgagg gcttcaagca ggcggtccgg
     2701 cagggcgcgg aattggctgc gcgcggccac atcgtcacgt tcggcatcac gccgcgatcc
     2761 ccagagacag gctatggcta catcaagcga ggcaccgcac tcggcgatga cgctttcacc
     2821 gtcgcgcagt tttgcgagaa gcccgacctt gcgcgcgcgg atgcgtttct tgcagacggc
     2881 tcctacgact ggaacagcgg catcttcatg gcccgcgccg acacactgac ggaagagatg
     2941 aaggtgcatt gccccgacat tgcggctcag tgcacgagcg ccgtcgccgg aagcaaccgc
     3001 gacatggact tcttgcgtct cgatgagcct gcctttgagg cgtgtccgtc ggcacccatc
     3061 gataccgccg tgatggagca cacctcgaag gccgtggtta tccccgtcga tatcggttgg
     3121 agtgatgtcg gctcctgggg cgcgctctgg gatctcggcg agaaggacga ggccggcaac
     3181 gtcatcagcg gcgacgtcgt cgcgctcgat accgaggaga cctacatccg cggcaatggc
     3241 cgcctcgtca caacccttgg cgtcaaggac ctcatcgtcg tcgatactgg ggacgcggtg
     3301 ctcatcgcgg acaaggcgcg ggtccaggac attcgcgatg tcgtgaccag gctcaaggag
     3361 cttggccgca gcgagcatga gacgcactcg cgcgtccacc ggccctgggg ttattacgag
     3421 tccatcgagg cgggcgaccg ctatcaggtc aagcatctga tggtgaagcc cggcgcatcg
     3481 ctatcgctgc aaatgcatca tcaccgggcc gagcactggg tcgttgtgaa gggcactgcg
     3541 cgcgtcgtac tcggcaacaa ggaaatgctg ctcagcgaga acgagtcgac ctacatcccg
     3601 atcggcacgc cccaccgtct ggagaaccct gggaaggttc ccttgagtat catcgaggtc
     3661 cagtccggct gctacctggg cgaggacgac atcgtgcgca tggacgatgt ctatgggcgt
     3721 ctggcggaga cgccctgatc gcaaagccga ctgcttctcc atacgcagaa ggagtactca
     3781 gcgaacttcg acggagccaa actcttttga cctaggcgca tgcaattttc gctttgggtt
     3841 agaagccgac atcgcccaac ctcaactcta ggtcagcttt tgacccaaag gtgacgtcct
     3901 acgagagcta gacttcagcc tagggctgtc cggctttagg gaccgaggcg gaaaagtggt
     3961 tcaaaatcag atgcaagagt tgctgcgaac gatgcagtca ggtgatggct atccctcatt
     4021 acgacccgac cgtcgagaat cgcgcgacac gtacctgatg ggcaaatgac gttgttcagg
     4081 tctactacct tgacccgttg ctcttcctcg tatgtcccgc gcggatacgc tgtctgcttt
     4141 ctgttccatc gacacgcatc ttcatttagc gggttttcaa gaaggcagat gatcggatct
     4201 ttgggtgagg tggtaatatc tctgatcagc acaatcgtag cattggtgtc ggccaggagc
     4261 tggtccacca acgatttctc tgcctcgatc agggcattct ctagctcttc cccgctcaag
     4321 cgttcccccg tggcatcgat gacgggagcg gaggaattgt tggcgagcag gataagttgc
     4381 gggtctagcc tcttgagctc agacaggaca tcgtcccgcc aactcgaaca ttccacataa
     4441 ggtcggctaa ggcggtcatt tgacaaaggc gcattgatgg aagaacagga agcctttgtc
     4501 cgagccagaa ggctccaacc agctttcttg gctgcaaggt cggccgcggg aagccacgat
     4561 gccgcatgcg agtccccgaa aaggacggct ctccgtgaac cggatgcatc cccataaatg
     4621 caaggcggtt gccttgtttc atcgtaggaa agcaagcatt catcagcttt gtctttccgg
     4681 atcgcttcag cattaagacg cttaccattg ctgagataga tcatcggttt agcgaaggtc
     4741 gcctgcgcta gtacagcgaa gcccgcggcg cccagagata agagaagacc ggccgtcacg
     4801 gagataccgg gagagcgcgt tagtgtcggc caataccggg ctggctgctc aatgacgtag
     4861 taggtaaggc cggccaaaag caatgaaaga aggacaagga atacgagcca tggtgtcgcc
     4921 gtggaatagg cggtgcccaa aaccaaaacg ggccaatgcc agaggtacca ggaataggac
     4981 catcgtccga cgaggacgac ggccggcaac ttgagtagac gtccgaccag accgccgatg
     5041 tcctctcttc gaccagcgag aatgagcgca gcggcgccag cagtgggtaa gacggcagcg
     5101 agtccgggat atggcgtttc gttggtgaac aggaccgccg caaagacaat tgctacaatt
     5161 cctgccaagg ccatccatcg gcgtgcgtat acatcgatcg acaccagggg cagaagtccg
     5221 atgactgcac cgatgccgag ctgccaagcg cgggagaagg tcccgaagaa ggcgtatggt
     5281 tggttgtggc cggttaggta gaggcaggtg ccgaatgaca tcagggtgat cgcgatggcc
     5341 gtccacatca ggacacgtgc gagtgccgtg ttccgaaaaa atctgacggc gacaatcgat
     5401 atcaagatca cgatcaaggg ccaaacggca taaaactgct cctcgatagc gagcgaccag
     5461 tactggagaa aaaggctcgg ctcggtgtta tcggcgaaat agtcgacggc cttgtcggcc
     5521 agacgccaat ttatgaaata gagcgctgcg gcaggcagtt ctttggctgc acggctctcg
     5581 agtgcgggcg gcagaaagat tgccgcgccg accagggacg cacacaggac caacacacca
     5641 gccggcagga tgcgccgcat gcggcgcgcc cagaaatcca tgagtcggat tgtgccgcgc
     5701 tcttcaaatt ctcgtaaaag caagctcgtg atgaggtagc ccgagatgac gaagaagacg
     5761 tcgacaccga cgaacccgcc tggagcaccc caaaagcccg cgtgaaacaa aaccacgagg
     5821 aaaaccgcta gtgcacgcaa accgtctatt tccgcacggt accgcaggcg aggaggcgcc
     5881 aaattgttcg cttgctgttc gatgtgatac tcccccccag ctcttcatcg cggggtctga
     5941 tcttgctgcg tgagacctga cggacttaga agtccctcaa cctctttgcc gtagcaagga
     6001 cgagttgcag gttttaacaa ttggagatga tacgcgatgg gcgtccttgt caccggtggg
     6061 gcgggctata tcggcagcca tatggcgctc gagcttctc
//
LOCUS       DBNHVU010000090         8224 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1521971, whole genome shotgun sequence.
ACCESSION   DBNHVU010000090 DBNHVU010000000
VERSION     DBNHVU010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8224)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8224)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8224
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1521971"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1179)
                     /locus_tag="AC16Y7_03395"
     CDS             complement(31..1179)
                     /locus_tag="AC16Y7_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HIR5708458.1"
                     /translation="MAIPEPTFTIGIEEEYLLVDKETRDLAPEQPPALLAKAEEALRG
                     QVSPEFLRSQIEVGTHVCRSVQEARDQLVELRSTIGAISGEFGLAPIAASTHPFASWE
                     SQHHTDKERYNVIARDLQHVGRRLLICGMHVHVGIEDDDLRIDLLGQAPYFLPHLLAL
                     STSSPFWQGVQTGLKSYRLSIFDELPRTGLPHKFSSYSEYERTIELLVSAGLIEDASK
                     LWWDMRPSARFPTLEMRITDVCPRIDDAVAIAALYLCILRLLYRLRRENKRWRHYPPF
                     LIRENRWRAQRYGIEKGMVDFGKGAVVPFSDLMEELFELVAEDAAHFDCSAEIAHVRT
                     ILQRGTSADRQLARYEEAKALGGTEHAALIAVVDELVEETQMSPAEKA"
     gene            complement(1228..1950)
                     /locus_tag="AC16Y7_03400"
     CDS             complement(1228..1950)
                     /locus_tag="AC16Y7_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009759800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIR5708459.1"
                     /translation="MPRLEHSHTPGAIAKRLRHGPRVSYLRDWVYGGIDGTVTTFAIM
                     AGVVGAELSATVVIVLGLANLLADGFSMAAGNYTGTKAEYDEYQQLRHMEERHIELAP
                     EGEREEIRQIFQAKGFKGEALESAVDVITKHRDQWIDMMMAEEHGMPAVTRSPVHAAL
                     YTFGAFVLCGSVPLLPYVAGMTAPELPSAVMAGVTFFAIGSVRSRWSPKSWWLAGLET
                     LAIGMLAASVAYFIGHLLRGIV"
     gene            2082..3473
                     /locus_tag="AC16Y7_03405"
     CDS             2082..3473
                     /locus_tag="AC16Y7_03405"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II 3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR5708460.1"
                     /translation="MSSRWTPDSWRTKPIEQVPVYMDPAALAEVEDQLASFPPLVFAG
                     EARELKAKLARVAQGEGFLLQGGDCAESFAEHGPDTIRDFFRVFLQMAVVLTYAGGSP
                     VVKVGRIAGQFAKPRSAPTETQDGIELPSYRGDIINGIDFTEESRSPDPQRMHMGYRQ
                     AAATLNLLRAFAQGGYANLEHVHQWNLGFVADSPAGHRYQELSDHIAETLRFMRAIGL
                     TPENTSQLRSTSLYTSHEALLLGYEQALTRVDSTSGDWYATSGHFLWAGDRTRQLDHA
                     HLDYLRGVKNPIGVKIGPSMTPDELIKMIDLLSPQNEPGRLTLICRFGADKVGDHLPA
                     LIRAVEREGKSVVWACDPMHGNTIKAASGYKTRPFDLILKEVEAFFDIHRSEGTHAGG
                     LHVEMTGSNVTECVGGAKALTDADLSDRYHTHCDPRLNADQSIELAFIVAERLRRERE
                     EHPQPEISIAAGE"
     gene            3523..5196
                     /locus_tag="AC16Y7_03410"
     CDS             3523..5196
                     /locus_tag="AC16Y7_03410"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIR5708461.1"
                     /translation="MTGDTRTSFKLAFAQLNPVVGDIEGNLDMARKARASLGKADADL
                     IVFTELYLTGYPLEDLVLKPALQLAAREACQALARETADGGPALLMGLPWGEGDAVYN
                     AVALLAEGRIEAVRYKVILPNYGVFDEKRVFASGPIPAPIEFRGLSLGVPICEDIWTD
                     EICQALVEAGAEILVVPNGSPYWMGKQATRYEVAETRVAETRRPLAYLNQVGGQDELV
                     FDGGSFVLNADGELAVQLPAWEEALVVTEWRREDSGWHCLTEPVAAVEEGEAANYLAC
                     VVGLRDYIDKNGFPGVVLGLSGGIDSALVAVMAVDALGADRVHCVMLPYEYTSQESLS
                     DAETIAKALHVRYDILPIHEGVDGLSKCLEDVLKGAPGDFTLENLQSRTRGTLLMAIS
                     NKLGPLVLTTGNKSEVSVGYATLYGDMNGGFNPLKDLYKGQVYALSRYRNVHRPKGCL
                     GPDGLIIPESVLTKDPSAELKPGQRDVDSLPPYPVLDDILNCLVEQEMPVPEIIARGH
                     APEIVKKVEKMLYLAEYKRRQAAPGVKITERNFGRDRRYPITNKFRERT"
     gene            5198..6535
                     /gene="gltX"
                     /locus_tag="AC16Y7_03415"
     CDS             5198..6535
                     /gene="gltX"
                     /locus_tag="AC16Y7_03415"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR5708462.1"
                     /translation="MTVTVRFAPSPTGRLHAGNIRTALINYLFARKAGGNFILRLDDT
                     DKERSTEAFARGIEEDVSWLGIEWAQLVHQSDRLARYEAAVERLKEMGRLYPAYETPE
                     ELELKRKRQLARGKPPVYDRAALKFSDEDKAKLEQEGRKPHWRFKLEPGDVRWNDLVR
                     GEQHVDASSLSDPVLIRADGTYLYTLPSVVDDIDLGVTHVIRGEDHVANTAPQIQLFE
                     AFGATLPAFGHHNLLVGANGQALSKRDRSLAIEGMRADGIEPLAVTSYAATVGTSDPV
                     APHASLDELAAAFDFAKLSRAPARFDPRDLRLLNAKLLHMLPFGIVAERLEAMGISGG
                     EPFWEAVGGNLEVLSDAKTWWAVVAGPLDPVIEDAGFCAAAADLLPPEPWDEATWGAW
                     TAAVKAKTGAKGKALFQPLRLALTGQSHGPELKVLLPLIGEGRARARLENKIA"
     gene            complement(6546..7505)
                     /locus_tag="AC16Y7_03420"
     CDS             complement(6546..7505)
                     /locus_tag="AC16Y7_03420"
                     /inference="COORDINATES: protein motif:HMM:NF022515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HIR5708463.1"
                     /translation="MQLQQELSAARGGGANAPELGMLDQQIAEQDRIYQSTKATMDSA
                     GCYERMFIFGRALKRTPKCLRMNNRVEDARRQLARLQQQRNSLQRGGGNRRRQAELQQ
                     ALRRNGCSGAPDTGGGGLFDFFGGPRQEYQTPIYRSINPNGRYRSVCVRLCDGFYYPV
                     SYSTYGSRVQQDAKKCQASCAAPAELYVYRNPGQEIEQAISLSGSAYMDLPVALRYRK
                     EFVKGCSCKQAEYNPTEIEAANKKAQAPAPATQANAAAAPAQPALSPDEMPKLNLDVG
                     GPATPGQPAALAAPPAPPPVAQQQAPAAPAPADGQSSVIKRSP"
     gene            7917..>8224
                     /locus_tag="AC16Y7_03425"
     CDS             7917..>8224
                     /locus_tag="AC16Y7_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR5708464.1"
                     /translation="MSLTLYNTLSREKEPFEPLDRAKVRIYVCGPTVYDYAHIGNARP
                     VIVFDVLFRLLRHLYGPEHVTYVRNITDVDDKINARAAEEYPDLPLNEAIRKVTEKTE
                     A"
BASE COUNT         1520 a         2452 c         2715 g         1537 t
ORIGIN      
        1 caacgtcgac ggcggcatgc agacgacgct ttaggctttc tccgcaggtg acatctgggt
       61 ttcctcgacg agttcgtcga ccacggcaat gagcgccgca tgctcggtgc cgcccagtgc
      121 cttcgcctcc tcgtagcggg ccagctggcg gtccgcactc gtgccacgtt ggagtatcgt
      181 gcgcacatgg gcgatttcgg cagagcagtc gaaatgagcc gcgtcctcgg ccacgagctc
      241 gaagagctct tccatgagat cggagaatgg aaccacggcg cccttgccga agtccaccat
      301 gcctttctcg ataccgtagc gctgcgcgcg ccaacggttc tcgcggatga gaaagggcgg
      361 gtagtggcgc cagcgcttgt tctcgcggcg caagcgatag agaagccgca ggatgcacag
      421 atataacgcg gcgatggcga ccgcgtcatc gatccgcggg cagacgtcgg tgatgcgcat
      481 ctcgagcgtg gggaagcgcg ccgacgggcg catgtcccac catagcttcg atgcgtcctc
      541 tataaggccc gcgctgacca gaagctcgat agtgcgttcg tactcgctgt agctcgaaaa
      601 cttgtggggc aggccggtac gcggcaactc gtcgaagatc gagaggcggt aggacttaag
      661 accagtctgc acgccctgcc aaaaaggtga cgacgtcgaa agtgccaaga ggtgcggcag
      721 gaagtagggg gcctgaccaa gaaggtcgat ccgcagatca tcgtcctcga tccccacatg
      781 cacgtgcatg ccgcaaatca ggagacggcg gcccacatgc tgcaagtccc tggcgatgac
      841 gttgtagcgc tctttgtcgg tgtggtgctg gctttcccag gaagcgaacg ggtgggtcga
      901 tgcggcgatc ggcgctaacc cgaattcgcc ggagatggca ccgatggtgg agcgaagttc
      961 gacaagctga tcgcgcgcct cttgcacgga cctgcacaca tgcgttccga cttcaatctg
     1021 cgagcgcagg aactcagggc tcacctggcc gcgcagcgcc tcttctgcct tggcaagaag
     1081 cgccggcggc tgctccggtg cgaggtcgcg cgtctccttg tcgacaagga gatactcctc
     1141 ctcgatgccg atcgtgaatg tcggttccgg aatcgccatg ggtcacactg ttgcacaatc
     1201 gatgtggctg cgggagagcc taaggcgtca gacaatccct ctcagcagat ggccgatgaa
     1261 ataggcgacg ctcgccgcaa gcatgccgat ggcgagtgtc tcaagaccgg cgagccacca
     1321 tgacttgggg gaccagcggc tacgcaccga gccgatcgcg aaaaaggtga cgccggccat
     1381 gacggcagac ggcaattccg gcgcggtcat tccggccaca tagggaagaa gagggacgct
     1441 gccgcacagc acgaatgcgc cgaaggtata gagcgccgcg tggacgggtg agcgggtcac
     1501 ggcgggcatg ccgtgctcct ccgccatcat catgtcgatc cactggtcgc gatgcttggt
     1561 gatgacgtcg acggcggact ccagcgcctc gcccttaaat cccttggcct ggaagatctg
     1621 gcggatctcc tcgcgctcgc cttcgggcgc gagctcgata tggcgttcct ccatgtggcg
     1681 gagttgctga tactcgtcgt attccgcctt ggtgcccgtg tagttaccgg ccgccatgga
     1741 gaagccgtcc gccaagagat tggccaaccc aagaacgatc acgaccgtgg ccgacagctc
     1801 ggcgccaacg acgcccgcca tgatggcgaa ggtggtgacg gtgccgtcga tgccgccata
     1861 gacccagtcg cgcaaatagc tgacgcgggg cccgtggcgg agccgtttgg cgatcgcgcc
     1921 gggcgtgtga ctgtgctcca gccgaggcaa aatctttttc cttccaacgg ccttaaggat
     1981 tatggggcaa gcttggcttg ggtcggcaaa ggccgcaagg ccgcctagcc acagtcattt
     2041 caatcgctta tatagcgggg acttaagtgg agagttgaag aatgtcatcg cgttggaccc
     2101 ctgatagctg gcggacgaaa cccattgagc aggttccggt ttacatggac ccggccgcgc
     2161 tcgccgaggt cgaggaccag cttgcctcgt tcccgccgct cgtttttgca ggcgaggcgc
     2221 gggagctgaa ggccaagctt gcccgcgtcg cgcaagggga gggctttttg ctccagggcg
     2281 gagattgcgc cgagagcttt gccgagcacg ggccggacac aatccgcgac ttcttccgcg
     2341 tattcctgca gatggcggtg gtgctgacct atgccggcgg atcgccggtg gtgaaggtcg
     2401 gccgcatcgc cggtcagttc gctaagccgc gttcggcgcc gaccgagacg caagacggca
     2461 tcgagctgcc gagctatcgc ggcgacatca tcaacggcat tgatttcacc gaggagtccc
     2521 gctcgccgga tccgcagcgc atgcacatgg gctatcgcca ggcggcggcg acgctgaacc
     2581 tgctgcgcgc gttcgcccaa ggcggctacg ccaacctgga gcacgtgcat cagtggaatc
     2641 tcggcttcgt cgccgatagc cccgccggtc accgctacca ggagctctcc gatcatatcg
     2701 cggagacgct gcgcttcatg cgtgcgatcg ggctgacgcc ggagaacacg tcgcagcttc
     2761 gctcgacgag cctttatacg agccacgagg cgttgctgct cggctacgag caggcattga
     2821 cgcgcgtcga ttccacgtca ggcgattggt acgctacgtc gggccacttt ttatgggcgg
     2881 gcgatcgcac gcgccagctc gaccacgcgc atctggatta tctgcgcggc gtcaagaatc
     2941 cgattggcgt gaagatcggc ccgagcatga cgccggatga gttgataaag atgatcgacc
     3001 ttttgagccc gcagaacgag ccgggtcggc tgacgctgat ttgccgcttc ggcgccgaca
     3061 aggtcggcga tcacctgccg gcgctgatcc gcgcagtgga acgcgagggc aaatccgtgg
     3121 tgtgggcttg cgatccaatg cacggcaaca cgatcaaggc ggccagcggc tacaagacgc
     3181 ggcccttcga tctgatcctg aaagaggtcg aggccttctt cgacattcac cggagcgaag
     3241 gcacgcatgc gggcggcctg cacgttgaaa tgaccggaag caacgtcacc gaatgcgtgg
     3301 gtggcgccaa ggcgctgacc gatgcggatt tgtcggaccg ctaccacacc cattgcgatc
     3361 cccggttgaa cgccgatcag tcgatcgagc ttgcctttat cgtagccgag cgcctgcggc
     3421 gggagcggga agagcatccg cagcccgaaa tatccatcgc ggctggcgaa taaggtggat
     3481 gtccgtctgg cgggcttgcg cgcccaccgc taggttcgat ccatgaccgg cgacaccagg
     3541 acctcattca agcttgcgtt tgcgcagctc aacccggtcg tgggcgatat tgagggcaat
     3601 ctcgatatgg cccgcaaggc gcgcgcgtcc ttgggcaagg cggacgccga cctgatcgtg
     3661 ttcaccgagc tctacctcac gggctatccg ctcgaggacc tcgtgttgaa gccggctctg
     3721 cagcttgcag cgcgggaggc gtgccaggcg ttggcgcgcg aaaccgcgga cggtggcccg
     3781 gcgctgctca tggggcttcc ttggggcgag ggcgatgcgg tctacaacgc cgtggcgctt
     3841 cttgccgagg gccgcatcga ggcggtccgt tacaaggtga tcctgccgaa ctacggcgta
     3901 ttcgacgaaa agcgtgtctt cgcgtcgggg ccaatcccgg caccgatcga attccgtggc
     3961 ctgtctctcg gcgtgccgat ctgcgaggac atctggaccg atgagatttg ccaggcgctg
     4021 gtcgaggccg gcgccgaaat actcgtcgtg ccgaacgggt caccctattg gatgggcaag
     4081 caggcgactc gttatgaggt agctgaaacc cgcgtggcgg aaacgcgccg gccgctcgct
     4141 tatctcaatc aggtcggcgg acaggatgag cttgtcttcg acggcggctc gttcgtgctc
     4201 aatgccgatg gggagctggc cgtgcagctt ccggcttggg aggaggcgct tgtcgtcacc
     4261 gaatggcgcc gcgaggatag cggctggcac tgtctgaccg aaccggtcgc ggccgtggag
     4321 gaaggcgagg ctgccaacta tctcgcctgc gtcgtggggc ttcgcgacta tatcgacaag
     4381 aacggttttc cgggcgttgt gctcggcctt tcgggtggca ttgattctgc gcttgtcgcg
     4441 gtgatggcgg tggatgcgct gggcgcggac cgcgtgcact gcgtgatgct gccgtatgaa
     4501 tacaccagcc aggaaagctt gagtgatgcg gagaccatcg cgaaggcgct ccacgtacgc
     4561 tacgacatct tgccgatcca tgagggtgtg gatggtttga gcaaatgcct ggaagatgtg
     4621 ttgaaaggcg cgccgggtga cttcacgttg gagaatcttc agtcgcgtac gcgtggcacg
     4681 ctgctcatgg cgatctccaa caagctcggt ccactcgtgc tgaccacagg caataaatca
     4741 gaagtgtccg tgggatacgc aacgctctat ggcgacatga atggcggctt caacccgctg
     4801 aaggatctct acaaggggca agtctacgcg ctgtcgcgct atcgcaacgt gcatcggccc
     4861 aaagggtgtc ttgggccgga cggcttgatc attccggaaa gcgtgctgac caaggatccg
     4921 tccgccgagc taaagccggg gcagcgcgat gtggatagtt tgccgccata tcctgttctc
     4981 gacgacatcc tcaattgctt ggttgagcag gagatgccgg tgcctgagat catcgcgcgc
     5041 gggcatgcgc cggagatcgt gaagaaggtc gagaagatgc tctatctcgc cgagtacaaa
     5101 cggcgccagg ccgcgcccgg cgtgaagatc accgaaagaa acttcggccg cgaccggcgc
     5161 tatcccatca ccaacaaatt ccgcgagcgg acttgagatg accgtgaccg tccgttttgc
     5221 gccgagcccg acggggcggc tccatgcagg aaatatccgc acggcgctaa tcaactatct
     5281 gttcgcccgc aaagcaggcg ggaacttcat attgcgtttg gacgacacgg ataaggagcg
     5341 ctcgacggaa gcgttcgcac gcggcattga ggaggacgtg tcctggctcg gcattgaatg
     5401 ggcgcagctc gtccaccagt cggatcgcct cgcgcgttac gaagcagccg tcgaaaggct
     5461 caaggagatg gggcggctct atccggctta cgagacaccg gaggagttgg agttaaagcg
     5521 caagcggcag ctcgcgcgcg gcaagccgcc ggtctacgac cgcgccgcgt tgaagttctc
     5581 ggacgaggac aaggcgaagc ttgagcagga aggccgcaaa cctcattggc gtttcaaatt
     5641 ggagccgggc gacgtgaggt ggaacgacct ggtgcgcggc gagcagcatg tggacgcgag
     5701 ctcactgtcg gacccggtgc tgatccgcgc cgatggcact tatctctaca cgctgccgag
     5761 cgtggtggat gacattgatc tcggcgtgac ccacgtcatt cgtggcgaag atcacgtggc
     5821 caacacggcg ccgcaaatcc agctcttcga ggcgtttggg gcgacgcttc ccgcttttgg
     5881 ccatcacaat cttctggtgg gcgccaacgg gcaggcgctg tcgaagcggg atcgttcact
     5941 cgcaatcgaa gggatgcggg ccgacgggat cgagccgctc gcggtcacaa gctacgcggc
     6001 cacggttggg acgtccgacc cggtcgcgcc ccatgccagt ctcgatgagc tggcggcggc
     6061 tttcgacttc gccaagctgt cgcgggcgcc cgcgcgcttc gatccccggg acttacggct
     6121 tctgaatgcg aagctgctgc atatgcttcc ctttgggatc gtggcggagc gccttgaggc
     6181 gatgggcatc tcaggcggag agccgttctg ggaggcagta ggcggcaatc tcgaagtgct
     6241 ttcggacgcc aagacatggt gggccgtcgt ggccgggccg ctcgaccctg ttatcgaaga
     6301 tgccggcttc tgcgccgccg ccgcggacct gctgccgccc gagccctggg acgaggcgac
     6361 ttggggtgct tggactgccg ctgtgaaggc gaagacgggc gccaagggca aggcgctgtt
     6421 ccagccattg cgcctcgcgc tcacgggcca aagccacggg ccggaactca aggttctgtt
     6481 gccactgatc ggcgagggcc gcgcgcgggc gcggctcgaa aacaaaatag cctaggagaa
     6541 gacgcttagg gcgaccgctt gatgacggag gattggccgt cggcaggcgc tggagcggcc
     6601 ggcgcctgtt gttgggccac cggcggcggc gctggcggtg cggccagcgc tgcaggctgt
     6661 ccgggtgttg ccggaccgcc gacgtcgaga ttgagcttcg gcatttcgtc cggcgaaagg
     6721 gcaggctggg cgggcgccgc agcagcgttg gcctgggtcg ccggagctgg ggcttgggcc
     6781 ttcttgttgg cagcctcgat ctcggtcggg ttgtattcag cctgcttgca ggaacagcct
     6841 ttgacgaatt ccttgcggta ccgcagcgcc accggcaaat ccatataggc cgagccgctc
     6901 aaggagatgg cctgctcgat ctcctgtccc gggttccggt agacgtagag ttcggcgggt
     6961 gccgcgcagc tcgcttggca ctttttggcg tcttgttgga cccggctgcc ataggtcgag
     7021 tagctgaccg ggtagtagaa gccgtcgcat aggcgcacgc agaccgatcg gtagcggccg
     7081 ttcggattga tgctgcggta gatcggcgtc tggtattcct gacgcgggcc gccaaagaaa
     7141 tcgaaaagac cgccgccacc agtgtctggg gcgccgctac agccattgcg ccttagggcc
     7201 tgttggagct cggcctggcg ccgccggttg ccgccgcccc gctgtaagga attgcgctgc
     7261 tgctggagcc gggcgagctg ccgccgggca tcttcaacgc ggttgttcat cctgaggcat
     7321 tttggggtgc gcttcagagc gcggccgaaa ataaacatcc gttcgtagca gccggcgcta
     7381 tccatggtgg ctttcgtgct ttggtagatg cggtcctgct cggcgatttg ctggtcgagc
     7441 atgccgagct cgggggcgtt cgccccgccg ccgcgggctg cggagagctc ctgttggagc
     7501 tgcatgcagc ggatgtcctg ttcggtctgc gccaggccag caccgggggc ggccagaaaa
     7561 cctaaagcga gtgcgagcgc tgtgggacga acaagcatcc gcacagacgt cacgacaacg
     7621 cgccgaaatt ggcgatgatc gctcacgatg gtttttttcc gttccccggg aagcctctaa
     7681 gccattatgc tagcttaaaa tcccgggtca atgaggcatc aataagatgg tttgcttgac
     7741 gatttggcgg tttcccgcca taagcggccc atgaaggcgc acccgcaacc gaaccttatc
     7801 atttccggct gcattattct gggctagcaa aacaagctcg ctcgggtcgt tcttccttta
     7861 actatcacag tcactccaac gaacatccgg cgggcccaag ccgaaggggc ttgtcagtgt
     7921 cgcttacgct ttacaatacg ctctcccgcg agaaggagcc tttcgagccc ctcgatcggg
     7981 ccaaggtgcg catctatgtg tgcgggccca cggtctatga ttacgcccat atcggcaacg
     8041 cgcggcccgt catcgtcttc gatgtcctgt tccgcttgct gcggcacctc tacgggcccg
     8101 agcatgtcac ctatgtacgc aacatcacgg acgtggacga caagatcaac gcgcgggcgg
     8161 cggaagaata tcccgacctg ccgttgaacg aagccattcg caaggtcacg gagaagaccg
     8221 aggc
//
LOCUS       DBNHVU010000091         7644 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1077275, whole genome shotgun sequence.
ACCESSION   DBNHVU010000091 DBNHVU010000000
VERSION     DBNHVU010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7644)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7644)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7644
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1077275"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..911
                     /gene="hemA"
                     /locus_tag="AC16Y7_03430"
     CDS             <1..911
                     /gene="hemA"
                     /locus_tag="AC16Y7_03430"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HIR5708465.1"
                     /translation="LLFTSAYIANDATLSTLARLIPGLVIFSDGKNHASMIEGIRHGG
                     GQKYIFRNNDLDHLEDLLKKVPLEAPKIIAFESVYSMDGHIAPIGEICELAQKYNALT
                     YLDEVHAVGLYGPCGGGIAELEGVMDRVDIINGTLAKGFGVMGGYIAASKSCCDAIRS
                     YAPGFIFTTSLAPAIAAGALASIRHLKVSELERARHRERVGTLKGLMAEARLPVMENR
                     SHIVPVMVGDPVHCKAVTDTLLTHYGIYVQPINYPTVPRGTERMRFTPSPVHTDAQMA
                     HLIDALNELWAACPVANGEYVRLAAE"
     gene            914..1180
                     /locus_tag="AC16Y7_03435"
     CDS             914..1180
                     /locus_tag="AC16Y7_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708466.1"
                     /translation="MHPRILARTLGRAIVIALGLTSSLAVASTQDATLCLLTAERVDE
                     GETLDEVGKQEAHEACQRALSATASVIQKYQFQEADFAITGAER"
     gene            complement(1208..1369)
                     /locus_tag="AC16Y7_03440"
     CDS             complement(1208..1369)
                     /locus_tag="AC16Y7_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708467.1"
                     /translation="MPIIIILLLAAMVATFGFWATLKGILGAIGVIILLCLLGVLTLS
                     FLAAWLMRK"
     gene            1454..2326
                     /locus_tag="AC16Y7_03445"
     CDS             1454..2326
                     /locus_tag="AC16Y7_03445"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR5708468.1"
                     /translation="MVRRAHLTEGISARGIETVDEDLTDLGSLSRLLALEDAALESDE
                     MRPLRLAREFSKADATLTERQISSTIVVFGGHLIVSPEDADAALARAEGPEQRARALR
                     MKELSAWYAEARLFARIVSERGGALATDAPRENVITTGGGPGIMEAANRGAMDAGAPS
                     IGFNIDLPEEQPPNPYTTPEHSFSFRYFAVRKMHFAIRANALAIFPGGFGTMDELFEI
                     LTLKQTRKTAPIPVILFSRDYWRTVVNVEALVDWGTIAPADADLFEMVDTAEEGWAAL
                     IRHGLMQEPPAKDV"
     gene            complement(2339..3220)
                     /locus_tag="AC16Y7_03450"
     CDS             complement(2339..3220)
                     /locus_tag="AC16Y7_03450"
                     /inference="COORDINATES: protein motif:HMM:NF025597.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HIR5708469.1"
                     /translation="MQERVVDEHQAEQAVSRWYLSRDGQQHGPLTDRELSVFAESGKF
                     QPGDLLWADGLDGWKPADAVFGLGSSQATAPAAPATPETTVDAGLSEADETSPDPVQT
                     ENEPISEDVDGEHVGAIVKALNGETEPPKLSPKERAMEELRKFSGTCAYLWVVFALLA
                     LHAWVGEARYGGTFSFFVLTTINAFWLMKLLPLVEDFGPLQELKRKPLVYSIVAKTVL
                     FSALLFAAYAVETIILGWIGGGGLQDAFSALAGGIAGTAMLWLIFCVALLPYFAFKEL
                     ERAVGADMIRKLLLGAR"
     gene            complement(3334..4761)
                     /locus_tag="AC16Y7_03455"
     CDS             complement(3334..4761)
                     /locus_tag="AC16Y7_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HIR5708470.1"
                     /translation="MAPQHDVIVVGSGAGGGSVAYKLVNVGKRVLLIEKGPFLPRDKS
                     TLEVREVFVDGVFKNHLVWLDSKGQDFVPGEFYNVGGKTKWYGAALFRFRPVEFEAEP
                     DFGLLGWPFGYDELKPYYDEATELLEITSFQNEPQLQALLDKITAADPNWKPHPLPLG
                     LSHDILSNPEEAKHFDGFASPSGYKSDSERNLIDQIRDKPNFAMLVGDPVAELIAAPG
                     DARTIIGVKCADGSTYEADKVVLAAGAMSSPRILEDHLHASGLDLPCGDLLGANFKMH
                     INSAVLGFSPFKDHDVLRKTAVLYNDKFPHSSMQCLGWIDPELLAVQAPPWTPGFVDK
                     FLGTRAIGFWATTEDSSSAENRIISGGPGGKPIMDYTIDRLPHAVKEHHAMIDNWIER
                     LLGAGLVGMDKYMGIAGTAHALGSMVTGDDPKSSVVDPRGKVHGMENLYVGDGSPLPR
                     ASRVNPSLTIYAWGLRLGEHLSSGA"
     gene            4958..5725
                     /locus_tag="AC16Y7_03460"
     CDS             4958..5725
                     /locus_tag="AC16Y7_03460"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5708471.1"
                     /translation="MPLKNKVAIVTGGNTGIGQAIVLELARRGASIVIDYVAHPEATE
                     ELERKVAALGDKCIGVDADVSKIADLEKLVGAAVGKFGRLDVLVNNAGIETRTSVLDT
                     TEDQYDAVLNINLKSAFFGTQLAAKQMLKQGGGGRIINVTSVHEDWPMPGNTAYCLSK
                     GGMRMLTRTAGVELAPHDILCVGVGPGAVETPINTSTMDDPAKLKKLDAAIPLGRMAK
                     PQEIASVVAFLAGDGASYLTATTIMADGGLMQSSPGL"
     gene            5738..7576
                     /locus_tag="AC16Y7_03465"
     CDS             5738..7576
                     /locus_tag="AC16Y7_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HIR5708472.1"
                     /translation="MTLPIEDYALIGDCETAALVGRNGSIDWLCLPRFDSEACFAAIL
                     GTEENGHWRIAATDPNAKITRRYRPDTLILETRFETKTGTATLTDFMYPVEPHPHIIR
                     FVECEEGKVEFETEFVVRFGYGEQVPWVTMLRDGRLRAVAGPNRVVLQSSVKLRGEDF
                     RTVGKFTVEPGKRETFALTYTESYIPEPPEADPQGLLVKTEQYWRGWTSQCNAEGPYA
                     DIVKRSLITLKAMTYAPTGGVVAAVTTSLPEQFGGPRNWDYRYCWVRDATLTLLALMN
                     TGYYEEARAWREWLLRAVAGSPKQMQIMYGIAGERRLYEIELPWLKGYENSTPVRVGN
                     AAHDQVQLDVYGELLDALHQARKGGLAPEQSGWELQLAIVEQLEEMWHQPDDGIWEVR
                     SGPEQFVYSKVMAWVAFDRSISSAERFKLRGPIARWRKTRDAIHKQVCDMGYDKDLKS
                     FMRSYGSKEVDASLLLIPAVGFLPATDPRVIGTVAQVEKTLMKDGLVQRYDTSKANDG
                     LPPGEGMFLACSFWLVDAYLMMGRQKDAVELFERLLALRNDVGLLSEEYDTTAKRLCG
                     NFPQAFSHLALVNSASNLGRDGRPVEQRCDTEEGTDAKGSAAAPST"
BASE COUNT         1514 a         2457 c         2349 g         1324 t
ORIGIN      
        1 cgctcctgtt caccagcgcc tatatcgcca acgacgcgac gctctccacg cttgcgcggc
       61 tgatccccgg gctggtgatc ttctccgacg ggaagaacca tgcctcgatg atcgagggca
      121 tccgccatgg tggtggccag aaatacatct tccggaacaa cgacttagac catctcgagg
      181 acctcctgaa gaaggttcct ctggaggcgc ccaagatcat cgcctttgag agcgtctatt
      241 cgatggacgg gcacatcgcc cctatcggcg agatctgtga gctagcccag aagtacaacg
      301 ccctcaccta tctcgacgaa gtgcatgcgg tcggcctcta tggaccctgc ggcggcggca
      361 tcgccgagct tgagggcgtc atggaccgcg tggacatcat caacggcacg ctggccaagg
      421 gattcggcgt catgggcggc tatatcgccg ccagcaaatc ctgctgcgac gccatccgct
      481 cctacgcgcc aggcttcatt ttcacgacct ccttggcgcc ggcgattgcc gcgggcgccc
      541 tggcgagcat ccgccacctc aaggtcagcg agcttgagcg cgcacgccat cgcgagcggg
      601 tgggcaccct caaggggctc atggccgagg cgaggctgcc cgtcatggag aaccggagcc
      661 atatcgtgcc ggtcatggtc ggcgacccgg tccactgcaa ggcggtcacc gataccctgc
      721 tgacccacta cggaatctac gtgcagccga tcaactaccc gaccgtgccg cgcggcaccg
      781 agcggatgcg gttcaccccc tctccggtcc acacggacgc ccagatggcc cacctgatcg
      841 acgctctgaa tgagctttgg gccgcctgcc ccgtcgccaa cggcgagtat gtgaggctcg
      901 cggcggagta ggcatgcatc cgcgcatcct tgcccgcacc cttggccggg caatcgtcat
      961 tgctctgggg ctgaccagtt cgcttgctgt tgcgtccacc caagacgcca cgctttgcct
     1021 cctcacggcc gaacgcgtgg atgaaggcga aaccctcgac gaagtgggaa agcaagaagc
     1081 ccacgaggcc tgccagcggg ccctgtcggc aaccgcgagc gtaatccaga aatatcagtt
     1141 tcaggaggcg gacttcgcca tcaccggagc cgaacgctag cccgggcaaa ccgaggcgcc
     1201 tagcccgcta cttcctcatg agccaggcgg ccaggaaaga cagggtgagg acgccaagca
     1261 ggcacagcag gatgatcacg ccgatcgcgc caaggattcc cttgagcgta gcccagaacc
     1321 caaatgtggc gaccatggcg gccagcagca ggataataat gatcggcatc ttagatttcc
     1381 ttcggccgct gagcgttcga gcttaccttt gcgcttttac cgcgcccgcc gcctcggctc
     1441 aataagagcg taaatggttc gccgtgcgca cctgacagag ggcatttcag caaggggcat
     1501 tgagaccgtg gacgaggact tgaccgattt gggctcactc agccggctcc ttgcgctgga
     1561 ggacgcggcg ctcgagagcg acgagatgcg ccctttgcgg ctcgcgcgcg aattctcgaa
     1621 ggccgacgcc acgctgaccg agaggcaaat ctcctcgacg atcgtcgtgt ttggcgggca
     1681 cctgattgtc tcgccggaag acgccgacgc tgccctggcc cgcgccgaag gacctgaaca
     1741 gcgggcccgc gccctgcgga tgaaggaact atcggcttgg tacgcggagg cgaggctatt
     1801 cgcgcgcatt gtgtcggagc gcggtggcgc gcttgcaacc gacgcgccac gcgagaacgt
     1861 aattacaacc ggcggcggcc ccggcatcat ggaagccgcc aatcgcggcg cgatggacgc
     1921 cggcgcgccg agcatcggct tcaacatcga tctgcccgag gagcagccgc ccaaccccta
     1981 tacgacgcct gagcacagct tctcgttccg ctacttcgcc gtccgcaaga tgcacttcgc
     2041 catccgcgcc aacgcgctcg ccatttttcc gggcgggttc ggcaccatgg atgagctgtt
     2101 cgagatcctg accctgaagc aaacgcggaa gaccgcgcca atccccgtga tcctgttttc
     2161 gcgggactat tggcgaaccg tcgtcaacgt cgaggctctc gtggactggg gcaccatcgc
     2221 acccgccgat gctgacttat tcgagatggt cgacaccgcc gaagaaggct gggcggccct
     2281 tatccgccat ggcttgatgc aggagccgcc ggcgaaagac gtgtgagggc agacggcctc
     2341 agcgggcgcc gagcagaagc ttgcggatca tgtcggcccc gaccgcgcgc tcaagctcct
     2401 tgaacgcgaa atagggaagc agcgcgacgc agaaaatgag ccagagcatc gccgtgccgg
     2461 caatgccgcc tgccagggcg ctgaaggcgt cctggagccc gccaccaccg atccagccaa
     2521 gaatgatcgt ttccactgca taagcggcaa agaggagcgc gctgaaaagg acggtcttgg
     2581 caacgattga gtagaccagc ggcttgcgct tgagttcctg caaggggccg aagtcctcca
     2641 ccagcggcaa gagcttcatc agccagaacg cgttgatggt ggtgagcacg aagaagctga
     2701 acgtgccccc gtatcgggcc tcgccgaccc aggcatgcaa cgccagcaag gcgaagacga
     2761 cccagagata ggcgcaagtt ccggagaact tccgaagctc ctccatcgcc cgctccttgg
     2821 ggctcagctt gggaggctcc gtctcgccat tcagcgcctt aacgatggcg ccgacatgct
     2881 cgccatcgac atcttccgat atgggctcgt tttcggtctg gacgggatcc ggcgatgtct
     2941 catcggcctc gctcaggccg gcatcgacgg tggtctcagg cgtggcgggc gccgcgggag
     3001 cagttgcttg ggaggaccca aggccgaaaa cggcgtcggc cggtttccag ccgtccaggc
     3061 catcggccca gagcagatcg ccgggctgga acttgccgga ctcggcaaaa acagaaagct
     3121 cgcgatccgt cagggggccg tgctgctgcc cgtctcggct taaataccaa cgcgacacag
     3181 cctgctccgc ctggtgctca tcgacgacac gctcctgcat gtaacccccc caagatcccc
     3241 gaatggccgg cgactgatta tcatcccggg cgaatggccg tcgagaggga tgttcgctac
     3301 cagttgacgc gcgggcggat ggcgaggggg cggctaggct ccgctggaga ggtgttcgcc
     3361 gaggcgcaag ccccaggcat agatcgtcaa ggagggattc acgcggctgg cccgcggaag
     3421 gggactgccg tccccgacat agagattctc catgccgtga accttgccgc gagggtcgac
     3481 cacggagctc ttcggatcat cgcccgtcac catgctgccc agcgcgtgcg ccgtaccggc
     3541 gatgcccatg tatttgtcca tccccacaag gcctgccccc aggagccgct cgatccagtt
     3601 atcgatcatc gcgtgatgct ctttcaccgc gtgcggcagg cggtcgatgg tgtaatccat
     3661 gatcggcttg ccgcccgggc cgcctgagat gatccgattc tcggcggagg acgaatcttc
     3721 cgtcgtggcc cagaagccga tcgcccgcgt gccgaggaac ttgtccacga agcccggcgt
     3781 ccacggcggc gcttgcacgg caagcagctc cggatcgatc caacccaggc actgcataga
     3841 cgagtgcggg aacttgtcgt tgtagagcac ggccgtcttg cgcagcacat catggtcctt
     3901 gaacggcgag aagccgagca ccgcggagtt gatgtgcatc ttgaagttgg cgccaagcag
     3961 gtcgccgcac ggcaggtcca acccgcttgc gtgaagatgg tcctcaagga tgcgcgggga
     4021 cgacatggcg cctgcggcga gcacaacctt gtcggcttcg taggtcgagc cgtcggcgca
     4081 tttgacgccg ataatggtcc gcgcatcgcc cggagcggca atgagttcgg cgacgggatc
     4141 gccgacgagc atcgcgaaat tcggcttgtc gcgaatctgg tcgatcaagt tccgctccga
     4201 atccgacttg tagccggagg gcgacgcgaa cccgtcgaaa tgcttcgcct cttccggatt
     4261 ggaaagaatg tcatgcgaca gccccagcgg cagcgggtgc ggcttccaat tcggatcggc
     4321 ggcggtaatc ttgtcgagca gcgcctggag ctgcggctcg ttctggaacg aagtgatctc
     4381 cagaagctcg gtggcttcgt cgtagtaggg cttcagctcg tcatacccga acggccagcc
     4441 tagaagccca aaatcgggct ccgcctcgaa ctccacgggc cggaaacgaa acagcgctgc
     4501 gccataccac ttcgtcttgc cgccgacatt gtagaactcg ccggggacga aatcctgccc
     4561 cttgctgtcg agccacacga ggtgattctt gaacacgccg tcgacgaaaa cttcgcgaac
     4621 ctccagcgtg gacttgtcgc gcggcaggaa cggccccttt tcgatcaaaa gaacccgctt
     4681 gcccacattg accagcttgt aagcgacgct tccgccgccc gcgccgctac caacaacgat
     4741 cacgtcatgc tgtggcgcca tgatgtgctc ccggtgagcc acgcccagag cgcgattttc
     4801 aagttgtaga ggcccgccga agtttggacc tttgcaaggt tgccgatgct atcgccaatg
     4861 tctaggctta tccttcgcca gtagcggtca aggtgagtcg ttacgctttt cagcgttagt
     4921 ggagtatgaa agtcatccac tccatggagc atcatctatg cccttaaaga acaaggtcgc
     4981 gatcgttact ggtggcaaca caggcattgg ccaggcgatt gttcttgagc tggcccggcg
     5041 tggcgccagc atcgtcatcg attatgtggc tcaccccgag gcgaccgaag agctggagcg
     5101 caaggttgca gcacttggcg acaagtgcat cggcgtcgac gccgatgtca gcaagatcgc
     5161 cgatctcgaa aagctggtcg gcgcagctgt cggcaagttc gggcgcttgg acgtcctggt
     5221 caacaatgcc gggatcgaga ctcgcacctc ggtcctcgac acaaccgagg atcaatacga
     5281 cgccgttctg aacatcaacc tcaagagcgc gttcttcggt acccagctcg ccgccaagca
     5341 aatgctgaag caaggcggcg gcggccgcat catcaatgtc acctcggtgc acgaagattg
     5401 gccgatgccc ggcaatacgg cgtattgcct atccaaaggc ggcatgcgga tgctgacccg
     5461 gaccgcgggc gtcgagctcg cgccccatga catcttgtgt gtcggtgtcg ggcccggcgc
     5521 ggtagagacg ccgatcaaca ccagcaccat ggacgatccg gccaagctca agaagctgga
     5581 cgccgccatc ccgctcggcc gcatggcgaa gcctcaggag atcgccagcg tggtcgcctt
     5641 ccttgccggc gatggcgcca gctacctcac ggccaccacc atcatggccg acggcggcct
     5701 gatgcaaagc agccccggcc tctagccgcg caccaccatg acgctcccca ttgaagacta
     5761 tgccctgatt ggcgactgcg agaccgcggc actggtcggt cgcaacgggt caatcgattg
     5821 gctctgtttg ccgcgcttcg actccgaagc ttgcttcgcg gcaatcctcg ggactgagga
     5881 aaacggccat tggcggattg cggcaaccga cccgaacgcg aagatcacgc gccgctaccg
     5941 gcccgacacg ttgatcctgg aaacacgctt cgagaccaag accggaaccg ccacgctcac
     6001 cgactttatg tatccggttg agccgcatcc gcacatcatc cgcttcgtcg agtgtgaaga
     6061 gggtaaggtc gaattcgaaa ccgaattcgt ggtgcgcttc ggctacggcg aacaggttcc
     6121 ctgggtcacc atgctacggg acggacgctt gcgcgcggtc gcaggcccca acagggtcgt
     6181 gctccagtct tccgtcaaac tgcgcggcga ggacttccgg accgtaggca agttcaccgt
     6241 ggagcctggc aagcgggaaa ccttcgcgct cacctacacg gagtcctaca tccctgagcc
     6301 gcccgaggcc gaccctcagg gcctgctcgt gaaaacggaa caatattggc gagggtggac
     6361 atctcaatgc aatgcagagg gtccatacgc ggacatcgtt aagcgctcgc tgattacgct
     6421 gaaagcgatg acctacgccc cgacaggagg cgtggtcgcc gcggtgacga catcgctgcc
     6481 cgaacagttc ggcggaccgc gcaactggga ctatcgctat tgctgggtac gcgatgcgac
     6541 gctgaccctg ctcgcgctca tgaacaccgg ctactacgag gaggcgcgcg cctggcgcga
     6601 gtggctgctg cgcgcggtcg caggcagccc gaagcagatg cagattatgt acggcatcgc
     6661 gggcgagcgc aggctctacg agatcgaatt gccctggctc aagggctacg agaactcgac
     6721 gcccgtgcgc gtcggcaatg cggcgcatga ccaggtccaa ctcgatgtct atggcgagct
     6781 gctcgacgcg ctgcatcagg cccgtaaagg cggcctcgca ccggaacaat ccggctggga
     6841 gcttcagctc gccatcgtcg agcagctcga agagatgtgg catcagccgg acgacggcat
     6901 ctgggaggtg cgcagcggcc cggagcagtt cgtctactcc aaggtcatgg cttgggtcgc
     6961 cttcgaccgc tccatcagta gcgccgaacg cttcaagctg aggggcccga ttgcccgctg
     7021 gcgaaaaacg cgggacgcca ttcataaaca agtctgcgat atgggctacg acaaggacct
     7081 caagagtttt atgcgctcct acggctccaa agaggtcgat gcgagtctgc tgctgattcc
     7141 ggccgtcggc ttcctgccag ccaccgaccc ccgcgtcatt ggaacggtgg cacaggtcga
     7201 gaagacgctg atgaaggacg gcctggtcca acgctacgac acatcgaaag ccaatgacgg
     7261 ccttcccccg ggagaaggca tgttcctcgc ctgcagcttc tggctggtgg atgcctatct
     7321 catgatgggc cgccagaagg atgcggttga gctgttcgag cggctcctgg ctttacgcaa
     7381 cgacgtcgga ctcttgagcg aggagtacga caccactgcc aagcgccttt gcggcaactt
     7441 cccacaagct ttctcgcacc tagccctcgt caacagcgcc agcaatctcg gccgcgacgg
     7501 caggcctgtc gagcagcgct gcgataccga ggagggcaca gatgcgaaag ggtcggctgc
     7561 ggcacccagt acctagtgtc cagatgagag caccctcccc cgaaccagaa catcggctgc
     7621 gcttcagggc acgcggtgcg gcgc
//
LOCUS       DBNHVU010000092         6296 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1819926, whole genome shotgun sequence.
ACCESSION   DBNHVU010000092 DBNHVU010000000
VERSION     DBNHVU010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6296)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1819926"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1268
                     /locus_tag="AC16Y7_03470"
     CDS             174..1268
                     /locus_tag="AC16Y7_03470"
                     /inference="COORDINATES: protein motif:HMM:NF018597.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin-like domain-containing protein"
                     /protein_id="HIR5708473.1"
                     /translation="MIRKPRVWLISACLLGAAAPASAQEVILTIPERRPLPAAAVDET
                     PQPARRPAAEDPVQNQAAPAPAAISAPAKIPEPKTTESDASTDIMAEDAAPDGVTPEA
                     ETETAERPPAGPPPLPPRKPPPGDRPTAAWNDVKVIAAREACDKALEGLTIDYERLQP
                     IRRGACGTPAPILVKSIGADLPVAIEPPATVQCALAAALDTWFNDQVQPAALDTFGSP
                     VVKIRNISSYKCRNRYNAANTKISEHAFANALDISGFELASGKKITVLKGWPRIVQPP
                     PPPEPNPDREVTPALTPVKSDPDAAFTPPPEASPPLGPELRDASEFLRAIHADGCKTF
                     NTVLGPNANAAHANHFHVDMKIRRYVTICE"
     gene            1368..2462
                     /gene="hemB"
                     /locus_tag="AC16Y7_03475"
     CDS             1368..2462
                     /gene="hemB"
                     /locus_tag="AC16Y7_03475"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003605713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIR5708474.1"
                     /translation="MSRRHVVSPSKDKTVSLRTERAKRSGTTPAPALQPGYPATRLRR
                     NRRSDWSRRLVRETTLTVDDLIWPIFVTEGTRERTEIDSMPGVARLSIDLAVNAAEDA
                     AAAGLPAIAIFPHTDPSLRDEAASQAFNPGNLVCRAVRAIKDAVPEIGIICDVALDPY
                     TSHGHDGLLTEGGEIDNDRTNAALITQALVQAEAGCDIVAPSDMMDGRIGAIRAALEA
                     NGFASTQILSYAAKYASAFYGPFRDAVGSGARLRGDKSTYQMNPANGDEALREVKLDI
                     SEGTDMVMVKPGIAYLDIVWRVKETFKLPTFVYQTSGEYAMLAAAAERGWLDRDRVIV
                     ESLIAFKRAGADAIFSYFAPEVARRLKDES"
     gene            complement(2459..3097)
                     /locus_tag="AC16Y7_03480"
     CDS             complement(2459..3097)
                     /locus_tag="AC16Y7_03480"
                     /inference="COORDINATES: protein motif:HMM:NF018178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-alpha-glucoside-1,2-lyase-like
                     domain-containing protein"
                     /protein_id="HIR5708475.1"
                     /translation="MRNKILALATAGLAIAGVLAASGEAIACVGNKVIFEDSFEDDAG
                     GWSHKAGTEMKDGAFVIKLPADDMQSNLNVTFSVKDADICTEAVWPTGGEQAVLGAGL
                     LFWGESNRTYYQFGILNNGRFWIARKQDGAWLGTIAANIDSPAIKKAPGSSNTLRVDA
                     KGNALTFYINGTKVREVRGQAPSSAWRFGVSGDNFDKEKEAIVQFTNMKVTD"
     gene            3276..3770
                     /locus_tag="AC16Y7_03485"
     CDS             3276..3770
                     /locus_tag="AC16Y7_03485"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR5708476.1"
                     /translation="MTGPRPSSAQTPAGLAAQRHAYVPATQPQLFDAVIRKRSVAFII
                     DAIIIALLWVVAVVAVSVLGVLTLGLAWLLFGLVFPLVGLGYNAVTIGGQHSSTIGQR
                     MMGLTVPMWFGGKVSPLIAAFHALLFWFSLVIFFPILLWAFFDPRKRCLHDILAGVVV
                     INRQ"
     gene            3911..4639
                     /locus_tag="AC16Y7_03490"
     CDS             3911..4639
                     /locus_tag="AC16Y7_03490"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HIR5708477.1"
                     /translation="MTEHKSNFPEFYITAPQPCPYLPGRLERKLFTHLTHDKPDFLVD
                     NLLKGGFRRSQNIAYMPYCDACSACVPVRILVDRFETRRSLKRIARANDDLVATRMPA
                     APSSEQYALFRDYIEARHADGGMAEMSVLDYSLMVEDSVVETFVTEYRLKPDETGDTP
                     LIAVALCDRLSDGISMVYSFYDPDMAARSLGTYMILDHVEFARRQGYPFVYLGYWIEG
                     SRKMRYKERFQPQERLTAKGWVRD"
     gene            complement(4646..>6296)
                     /locus_tag="AC16Y7_03495"
     CDS             complement(4646..>6296)
                     /locus_tag="AC16Y7_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR5708478.1"
                     /translation="IKFPKATADKLVGFVPGPDLPTGGIIVEPRENIVEAYKTGRGSF
                     RLRARWEQEDTGRGTYQIVITEIPYQVPKARLVARIAELLQAKKLPLLADVRDESAED
                     VRLVLEPKSHTVDPVLLMESMFKLTELETRVPLNMNVLSHGKIPNVIGLRQVLQEWLD
                     HRLEVLVRRSKFRLAKIDHRLEVLAGYLIVYLNLDKVIKIIREKDEPKPALMKAFKLT
                     EVQAEAILNMRLRALRKLEEMEIKRENEDLTKERKGLNALLRSKDKQWKRISDEIKDV
                     KAQFGQKTEVGKRRTSFAEAPDEDVAIELAEAMIEKEPITVVLSEKGWIRSIKGHNTE
                     PSSLSFKEGDRLKSFVLAQTTDTLLLFATNGRFYSLAADKLPGGRGHGEPFRLMVDIE
                     DAGDCVELFVYEPGGQLLVASTSGYGFVVTEDDVVAMTRKGKQVLNVKGDDEAAVCAP
                     VEGDSVAVIGENRKLLLFPLKELPEMPRGKGVRLQRYKDGGLADAKIFTKKEGLPYTV
                     GAGRNFLFEPSELRDWWGARAQAGRLPPRGFPKSGTFGERF"
BASE COUNT         1140 a         2099 c         1868 g         1189 t
ORIGIN      
        1 agggcggtcc aaggtgacaa ccccggcccg gtcatgctct ttgaacgtga tgccaagtgc
       61 ttcggacaaa cgcgataatc cttgattcat atcctgtctg ggggaaactc gcggcagcta
      121 gcatcccgtg gcccaaacct caagcgctgg cgaagacgct aaggcagtca tcgatgatcc
      181 gtaagccccg cgtttggctc atttcggcct gtcttcttgg cgccgcggca cccgccagcg
      241 ctcaggaggt catccttacg atccctgaga ggcggccgct accggcggca gcggtagacg
      301 agacgcccca gccggcccga agacccgcag ccgaggatcc ggtacaaaac caggccgcgc
      361 ccgctccagc cgccatttcc gcccccgcaa aaatacctga gcctaagacg accgaaagcg
      421 atgcttctac ggacatcatg gctgaggacg ccgcgcctga tggcgtaacg cccgaagcag
      481 agaccgaaac ggcagagaga ccaccggctg ggccgccgcc cttgccgccc agaaagccgc
      541 ctcccggcga ccgcccgacg gcggcttgga acgacgtgaa agtcatcgcc gcgcgcgagg
      601 cttgcgacaa ggcgctcgag ggcctgacca tcgactatga acgattgcag ccgatcagac
      661 ggggcgcgtg cggcacgccg gcgccgatcc tggtgaagtc gatcggtgcg gatctgcccg
      721 tggcgatcga gccgccggcc acggtccagt gcgctctcgc cgccgcgctg gacacctggt
      781 tcaacgatca ggtgcagcct gccgcgctcg acaccttcgg cagccccgtc gtcaagatcc
      841 gcaacatctc ctcctacaag tgccggaacc gctacaacgc ggccaacacc aagatcagcg
      901 agcatgcgtt cgccaacgcg ctcgacattt ccgggttcga gctcgcctcg ggcaagaaga
      961 tcaccgtgct caagggctgg ccgcgcatcg ttcagccccc gccgccgccg gagcccaatc
     1021 ccgaccgcga ggtgacgccg gcgctgaccc cagtcaagtc cgaccccgat gcggccttca
     1081 cgcccccgcc tgaagcctcg ccgcctctag gtccggagct ccgcgatgcg tcggagttct
     1141 tgcgggccat ccacgccgac ggctgcaaga cgttcaacac cgtcctcgga cccaacgcaa
     1201 atgcggccca tgcgaaccat tttcatgtcg acatgaaaat acgtcgctac gtgacgattt
     1261 gcgaatgaca ccggaaaggg ccgggaaagc ccggaaaacc atgttggcaa gtgcttctgg
     1321 tagcccgttg cgggcagtga taagatcggt cccttcggcg gaagtttatg agtagacgac
     1381 acgtggtctc accgtccaaa gacaaaacag tttccttgcg gaccgagcgc gccaagcgca
     1441 gcgggacgac accggcgccc gcgctccagc ccggctatcc ggcgacgcgc cttaggcgta
     1501 accgcaggtc cgactggtcc cgccgtctcg tgcgagagac gacccttacc gtcgacgacc
     1561 taatctggcc gatcttcgtc accgagggga cacgcgagcg cacggaaatc gactccatgc
     1621 ccggcgtggc gcgcctgtcc atcgacctcg ccgtcaatgc cgcggaggac gccgccgccg
     1681 ccggactgcc tgcgatcgcg atctttcctc acaccgatcc gtcgttgcgc gacgaagccg
     1741 cgagccaggc cttcaatccg ggcaatctcg tgtgccgcgc ggtgcgcgcc atcaaggacg
     1801 ccgtccctga gatcggcatc atttgcgacg tggccctcga tccctacacg agccacggcc
     1861 acgacggact tctcaccgaa ggcggcgaga tcgacaacga ccgcaccaat gccgcgctga
     1921 tcacccaggc gctggttcag gcggaagccg gctgcgatat cgtcgccccc tccgacatga
     1981 tggatggccg cataggcgcc attcgtgccg cgctcgaggc caacggtttt gcaagcaccc
     2041 aaatcctgag ctacgcggcg aaatacgctt ccgcgttcta cggaccgttc cgcgacgcgg
     2101 tgggttcggg cgcgcgactg cgcggcgaca agagcaccta tcagatgaac ccggccaacg
     2161 gcgacgaggc gttgcgcgag gtcaagctcg acatctccga aggcaccgac atggtcatgg
     2221 taaagccggg catcgcttat ctcgacatcg tgtggcgggt gaaggagacc ttcaagctgc
     2281 ccactttcgt ctatcagacg tccggggagt acgcgatgct ggcggcagct gcagagcgcg
     2341 gctggctgga ccgcgatcgc gtcatcgtcg aaagcttgat tgcttttaag cgcgccggcg
     2401 cggatgccat cttcagctac ttcgcgcccg aagtggcacg tcgcctgaaa gacgagagct
     2461 agtccgtcac cttcatattg gtgaactgga cgatcgcttc cttttccttg tcgaaattgt
     2521 cacccgagac gccaaagcgc cacgccgacg acggtgcttg gcccctcacc tcgcgcacct
     2581 tggtgccgtt gatgtagaag gtcagcgcgt tgcctttggc gtcgacgcgc agcgtgttgg
     2641 atgagcccgg cgctttcttg atcgcagggg aatcgatatt ggcggcgatg gtgccgagcc
     2701 aggcaccgtc ctgcttgcgg gcgatccaga accggccgtt gttgagaatg ccgaattgat
     2761 agtaggtgcg gttgctctcg ccccagaaca acagccccgc gcccagcaca gcctgctcgc
     2821 cgcctgtcgg ccaaaccgct tcggtgcaga tatcggcatc cttcaccgag aaggtgacgt
     2881 tgaggttcga ctgcatgtcg tcggctggca gcttgatgac gaaggcgccg tccttcattt
     2941 cggtgccggc cttgtgggac cagccgcctg catcgtcctc gaaactatct tcgaagatca
     3001 ccttgtttcc cacgcaggca atcgcctcgc cgctcgcggc gagcacgccc gctatcgcaa
     3061 gcccggcggt cgcaagcgcc aggatcttat tccgcatcgt gcggctcccc gttcgttcgt
     3121 ggcatccaca gtgagtccta aactgtcgtc ataaactcgg caagctgcgc gcccgttcca
     3181 tgcgcgagca gcatgttggc aaagacttgc ggttgtttgg agccattcaa atccccatat
     3241 ttacgacacg cacacagccc cgattcggag atttcatgac tggcccccgc ccttcctctg
     3301 cccagacccc agccggctta gcagcacagc gccatgccta cgttccggca acgcagcctc
     3361 agctgttcga cgcggtcatc cgcaaacgct ccgtggcgtt catcatcgac gccatcatca
     3421 ttgctctgct gtgggtcgtt gccgtggtcg cggtctcggt cctcggcgtg ctgacgctgg
     3481 gccttgcctg gctgcttttc ggactggtct tcccgctcgt gggcctcggc tataacgccg
     3541 tcaccatcgg cggacagcat tcgtccacga tcggccagcg catgatgggg cttaccgtgc
     3601 ccatgtggtt cggcggcaag gtctcgcctc tgatcgcagc cttccatgcg ctgctgttct
     3661 ggttctcgct ggtgatcttc ttccccatcc tgctgtgggc cttttttgac ccgcgcaagc
     3721 gctgcctgca cgacatcctg gcgggcgtgg tcgtcatcaa ccggcaataa ttggagcgat
     3781 tccaagtttt tgcgggccga agcatcgccg attgcgcgcc ccgccatttg tccactaagc
     3841 ttggcgagga ttgacttgga ttcgcttccg gtcggtgaca cgtgacagag cacaaaagta
     3901 actttggccc gtgacagagc acaaaagtaa ctttccggag ttctacatca cagctccgca
     3961 gccctgccca tatctgccag ggcggctgga gcgaaagctc ttcacgcatc tcacgcacga
     4021 caagcccgac tttctcgtcg acaatctctt gaagggcggc ttccggcgca gccagaacat
     4081 cgcctacatg ccctattgcg atgcctgctc ggcgtgcgtg ccggtccgca tcttggtgga
     4141 tcggttcgag acgcggcgct cgctgaaacg catcgcgcgg gccaatgacg atctcgtcgc
     4201 cacgcgcatg ccggccgcgc cgagttcgga acaatatgcg ctgttccgcg actatatcga
     4261 ggcacgccac gccgatggcg gcatggccga gatgagcgtg ctcgattact cgctcatggt
     4321 cgaggacagc gtggtggaga cgttcgtcac cgagtacagg ctgaagccgg acgagacggg
     4381 cgacacgccc ctgatcgccg tggcgctgtg cgatcgctta agcgacggca tctccatggt
     4441 ctacagcttc tacgatcccg acatggccgc gcgcagtctc ggaacctaca tgatcctcga
     4501 ccatgtggaa ttcgcgcggc gccaaggcta tccgttcgtc tatctcggct attggatcga
     4561 gggctcgcgc aagatgcgtt acaaggagcg cttccagccg caagaacggc ttaccgccaa
     4621 aggttgggtt cgcgactaga ggcgctcaga agcgctcgcc gaacgttccc gattttggaa
     4681 agcctcgcgg cggcaggcgg ccggcctgtg cgcgggcgcc ccaccaatcg cgcagctcgg
     4741 acggctcaaa gagaaagttg cggcccgccc caaccgtgta gggcagcccc tccttcttgg
     4801 tgaaaatctt ggcgtcggcc agaccgccat ccttgtagcg ttgcaggcgc acgcccttgc
     4861 cgcgcggcat ctcgggcagt tccttcaacg ggaacagcag cagcttccgg ttctcgccga
     4921 tgaccgcgac gctgtcgccc tccaccggcg cgcatacggc cgcctcgtcg tcgcccttca
     4981 cgttcaagac ctgcttgccc ttgcgggtca tggcgacgac gtcgtcctcg gtcaccacaa
     5041 aaccatagcc gcttgtggat gccaccagaa gctggccgcc cggttcatag acgaacagct
     5101 cgacgcaatc gcccgcgtcc tcgatgtcga ccatgaggcg gaagggctcg ccgtgccctc
     5161 tgccgcccgg cagcttgtcg gcggcgagcg aatagaaccg gccattggta gcgaacaaca
     5221 gcagcgtatc cgtggtttgc gcaagcacga agctcttcag ccggtcgccc tccttgaacg
     5281 aaaggctcga gggctcggtg ttgtgaccct tgatcgagcg gatccagccc ttctccgaca
     5341 gcacgacagt gatcggctct ttctcgatca tcgcctcggc gagctcgatg gcgacgtctt
     5401 catccggcgc ttcggcgaag ctggtgcggc gcttgccgac ttccgtcttc tgcccgaact
     5461 gcgctttcac gtccttgatc tcgtcggaga tgcgcttcca ctgcttgtcc ttcgagcgaa
     5521 gcagcgcatt cagtcccttt cgctctttgg tgagatcctc gttctcgcgc ttgatctcca
     5581 tctcttcgag cttgcgcaag gcacgcaggc gcatattcaa aatggcctcg gcctgcacct
     5641 cggtgagctt gaaggccttc atcaatgccg gcttgggctc atccttctcc cggatgatct
     5701 tgatgacctt gtcgagattc aaatagacga tgagatagcc ggcgagcact tcgagccggt
     5761 ggtcgatctt ggcgagccgg aacttggagc ggcggaccag aacctcaagc cggtggtcga
     5821 gccattcctg cagcacctgg cgcaagccga tcacgttcgg gatcttgccg tggctcagca
     5881 cattcatgtt gagcggcacg cgcgtctcaa gctccgtcag cttgaacatg gactccatga
     5941 gcagtacggg atcaaccgtg tggctcttcg gctccagcac gaggcgcacg tcttcggcgg
     6001 actcgtcgcg gacgtcggcg agaagcggca gcttcttggc ctgcagcagc tcggcgatgc
     6061 gggcgacgag acgcgccttc ggcacctgat agggaatctc ggtgatcacg atctgatagg
     6121 tgccgcggcc ggtgtcctcc tgctcccagc gcgcacgcag gcggaaggaa ccgcggcccg
     6181 tcttgtacgc ctcgacgata ttctcgcgcg gctcgacgat gatgccgccg gttggcagat
     6241 ccgggcccgg cacgaaaccc accagcttgt cggccgtggc cttcgggaac ttgatg
//
LOCUS       DBNHVU010000093        10424 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_557250, whole genome shotgun sequence.
ACCESSION   DBNHVU010000093 DBNHVU010000000
VERSION     DBNHVU010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 10424)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10424)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10424
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_557250"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..2315
                     /gene="uvrB"
                     /locus_tag="AC16Y7_03500"
     CDS             48..2315
                     /gene="uvrB"
                     /locus_tag="AC16Y7_03500"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR5708479.1"
                     /translation="MIQEHPLVTGETAAFTPHRPSRPEKSEGGVALQTETELEPKGDQ
                     PHAIAELVSQVNARERDQVLLGVTGSGKTFTMAKVIEETQRPALILAPNKTLAAQLYG
                     EFKSFFPENAVEYFVSYYDYYQPEAYIPRTDTYIEKDSSINEQIDRMRHSATRALLER
                     DDVIIVASVSCIYGIGSVETYTAMTFTLKRGDNIEQRQLMADLVALQYRRNDVNFVRG
                     SFRVRGDTIELWPAHLDDRAWRVSLFGDEIESIVEFDPLTGAKTDEFSLVKVYANSHY
                     VTPKPTLKQAVRGIKEELKQRLTELHGAGRLLEAQRLEQRTLFDLEMIEATGSCAGVE
                     NYSRYLTGRKPGEPPPTLFEYLPDNALVFTDESHVTVPQIGAMFRGDYKRKSTLAEYG
                     FRLPSCMDNRPLRFEEWDAMRPQTVYVSATPGGWELEQTGGVFAEQVIRPTGLVDPPV
                     EVRPATTQVDDLIDETRKVVDAGYRVLVTTLTKRMAEDLTEYMHEQGIRVRYMHSDVE
                     TLERIEIIRDLRLGAFDVLVGINLLREGLDIPECALVAILDADKEGFLRSETSLVQTI
                     GRAARNVDGRVILYADGITGSMERAMAETDRRREKQVAWNKANGITPESVKKNIGDIL
                     ASVYERDHVLVDAGTGFSEEDQMPIGHNLESVIADMEKRMRASAADLEFEEAARLRDE
                     VKRLRQTELAIANDPLARQSEVERSAAAADISRPHKPDLDEMGPHNREIPLKTARPRS
                     KSGKPGTRAFKGKGH"
     gene            2807..3250
                     /locus_tag="AC16Y7_03505"
     CDS             2807..3250
                     /locus_tag="AC16Y7_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR5708480.1"
                     /translation="MSALIDWLEAGRRLAERIPYSIVALVARLAVANVFWRSAQTKIN
                     GVFEVKDSTFYLFSEKYHVPILPPDVAAYIATYQELVGSVLLVVGLATRLTALAFIGM
                     VAVIQIFVYPSGWPDHILWFALLFLLVARGPGAISLDHLIWRRTK"
     gene            complement(3286..4518)
                     /locus_tag="AC16Y7_03510"
     CDS             complement(3286..4518)
                     /locus_tag="AC16Y7_03510"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HIR5708481.1"
                     /translation="MARYSLTRIISTSALAGLCAAVLLGSVSTAFAQRCPGPSGFNKW
                     LHGFKKQAIAEGISPQTVAAALNGVTYDPRTIKKDRAQGVFAQDFNTFSRRMVSKNRL
                     QAGKARMRKYGKTLKQVEQQYGVPGPVLVAFWGLETDFGAFMGDMQSIRSLATLSYDC
                     RRPEEFRVQLMDALRVIDRGDLTPSQMRGPAHGEVGQFQFQPSTYYKYAVDYDGDGRR
                     DLVRSAGDSLASAANYIRAKGWQPGQPWLEEVIVPKNLPWDQADVTIQRPRLFWSRYG
                     VTYRNGKPVPADNVPSALLLPMGRNGPAFLAYPNFINIYLDWNNSLIYSTTAGYFATR
                     LGGAPPLRRGNAPVFSAAQNKQLQRLLQQRGYDVGKIDGVIGAGTRAAIRDMQVKYGL
                     PADSYPSQALIRKLQAGG"
     gene            complement(4622..4858)
                     /locus_tag="AC16Y7_03515"
     CDS             complement(4622..4858)
                     /locus_tag="AC16Y7_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3072 domain-containing protein"
                     /protein_id="HIR5708482.1"
                     /translation="MQQKKTASAYQERQELAPSGSTGAEPMTGAQASHLKNLAEEVRE
                     PEAYRQGLSKAEASRRINVLTEKLRIGELPPHTD"
     gene            5302..5661
                     /locus_tag="AC16Y7_03520"
     CDS             5302..5661
                     /locus_tag="AC16Y7_03520"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HIR5708483.1"
                     /translation="MVKVRAKSIAAGLLGAALMVTVSAAMADQVGSASWYALTSKTAS
                     GERMNPNALTAAHRSLPFGSKVLVENLRNGRSVVVRINDRGPFTGGRIIDLSKAAAAQ
                     IGMINSGTAKVRMKRIK"
     gene            5955..6260
                     /locus_tag="AC16Y7_03525"
     CDS             5955..6260
                     /locus_tag="AC16Y7_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708484.1"
                     /translation="MLTRPALRTILPYAAFLIVASLTFVAFDTAVAQQQQSQRGSNQG
                     LFKKPHRGPAIGGTSWTLSDVWGEPRMPEPSYDYPGFNYSPGGYTLDGAPNTAPYPN"
     gene            6468..6683
                     /locus_tag="AC16Y7_03530"
     CDS             6468..6683
                     /locus_tag="AC16Y7_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR5708485.1"
                     /translation="MRQTGTVKFFNHSRGFGFITPDDGGKDVFLHISALQQSGLPALD
                     EGAKVSFETEPDRRGKGPQAINVQMGE"
     gene            6817..8247
                     /locus_tag="AC16Y7_03535"
     CDS             6817..8247
                     /locus_tag="AC16Y7_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5708486.1"
                     /translation="MTVDRTRASDQLRSVLDRLTEGRAAALERLFDFLAIPSISTDPA
                     CHEACLYAADWCAKSLCDIGFEARVEPTDGKPMVVAHWRHPDGGARPHVLFYGHYDVQ
                     PPDPLEAWDAPPFEAQLVDDAQHGQVIVARGASDDKGQVMTFLEACRAWMAVDGALPV
                     DVTVLFEGEEESGSPSLAPFLAAHGDELQADIALVCDTGQWDPETPAITAFLRGLAFS
                     EITVSGPSRDLHSGIYGGPARNPIRVLAELVADLHDAQGRVTIPGFYDDVHEPSGKQR
                     DAWGALGFDTSAFLGEIGLSEPAGETDRNVLEQLWSRPTVEVNGMIGGYTGPGTKTVI
                     PAQASAKLSFRLVPNQDPAKIIDGLHRFIAERLPGDARVSFGGEGGSPAVGFDTGAPA
                     FRATAAALQDEWGKPPVVVGCGASIPIVESFRTRLGMDALLVGFALDDDRIHSPNEKY
                     NLTSFEKGARSWARILAKLGDHDVSL"
     gene            8234..8929
                     /locus_tag="AC16Y7_03540"
     CDS             8234..8929
                     /locus_tag="AC16Y7_03540"
                     /EC_number="4.6.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012659.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0033897 - ribonuclease T2 activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T2 family protein"
                     /protein_id="HIR5708487.1"
                     /translation="MSRFDGCITGVGAAAIAATLLCASAGVAYDDRPHAYRHAEPGNI
                     DYYALVLSWSPTHCLMEGGPRGDQQCDAKQPDDFVLHGFWPQYAKGWPEDCYTGQRPW
                     VPSAVINEMLGVMPSKGMIIHEYRTHGTCSGLSPADYFAAARIARDQVHIPAIFDDPK
                     TQHFLSPEKIESEFIATNDWLTPDMIAVTCRRGKLFDVRICFGTDLQPRACGSNFNQK
                     RLCPLRRVTVTVP"
     gene            complement(8926..9609)
                     /locus_tag="AC16Y7_03545"
     CDS             complement(8926..9609)
                     /locus_tag="AC16Y7_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HIR5708488.1"
                     /translation="MRTRLYRAATGAVVLVASATASSPALSGGDVYDTAPVYDMAPVP
                     SQDLFEGWWLGGTIGGASVSYDKTPAGSVSSDGAVGGIIGGYSWQHGPFVVGIEGDFL
                     ASDVSGSRRFGGGLNKVSPGFDSVADLRLRAGYTVLPNLLLFGTFGGSWADADLPISG
                     PGGAFREATFFGWSVGGGAEYAFDQNWSGRFDYQFTDFDAETVSYPGGKATFDPDSDT
                     FRGSVIYRF"
     gene            complement(9800..>10424)
                     /locus_tag="AC16Y7_03550"
     CDS             complement(9800..>10424)
                     /locus_tag="AC16Y7_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016917666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase type 12"
                     /protein_id="HIR5708489.1"
                     /translation="KAHAVYTPAALRFYDLVVHKLSNRFAWRCPTSRLDELYARNLPA
                     NHLEAGVGTGLFLDRAGTGFDRLVLADINVHCLDLAGRRLARFRPALRQANLLAPLEF
                     DLAPFTSVGLTYVLHCLPGTMSEKLVAVDHLKPFMAEGARLFGATILGRGVVPNIAAR
                     GLLDLYNKKGVFNNGADGIEALTDGLRQRFATVRIEQDGLVALFTAR"
BASE COUNT         2004 a         3391 c         3160 g         1869 t
ORIGIN      
        1 ttgccggcga ccggcgcgat ccgggattcg gcgaagcgcc ccaaccgctg atccaggagc
       61 atccgctggt cacgggcgag acggccgcgt tcacgccgca ccggccatcg cggccagaga
      121 agtccgaagg cggcgtcgcc cttcagaccg agacggaatt ggagcctaag ggcgaccagc
      181 cccacgcgat cgccgaactc gtcagccaag tgaacgcccg tgaacgcgat caggtgctgc
      241 tcggcgtcac gggctcgggc aagaccttca ccatggccaa agtcatcgag gagacgcagc
      301 gcccggcact catcctcgcg ccgaacaaga cgcttgccgc gcagctctac ggcgagttca
      361 agagcttctt cccggagaac gcggtcgagt atttcgtctc gtactacgac tactaccagc
      421 ccgaggccta tatcccgcgc accgacacct atatcgagaa ggactcgtcc atcaacgagc
      481 agatcgaccg catgcgccac tcggcgacac gcgcgctgct cgagcgcgac gacgtgatca
      541 tcgtggccag cgtgtcgtgc atctacggta tcggctcggt ggagacctat accgccatga
      601 ccttcaccct gaagcgcggc gacaatatcg aacagcgcca gctgatggcc gatctggtcg
      661 cccttcaata ccggcggaac gacgtcaact tcgtgcgcgg gagcttccgg gtccgcggcg
      721 ataccatcga gctatggccc gcccatttgg acgatcgcgc ctggcgagtc tcgctgttcg
      781 gcgacgagat cgaaagcatc gtcgaattcg atcctctgac cggcgccaag accgacgagt
      841 tcagcctggt caaggtctac gccaattcac actatgtgac cccgaagccg acgctgaagc
      901 aggccgttcg cggcatcaag gaggagctga agcagcgtct caccgagttg cacggcgccg
      961 ggcgcctgct cgaggcgcag cggctggagc agcgcaccct gttcgatctg gagatgatcg
     1021 aggcgaccgg ctcctgcgcc ggcgtcgaga attactcgcg ctatctcacg ggccgcaagc
     1081 ccggcgagcc gccgcccacg ctgttcgagt acctgcccga caatgccctc gtctttaccg
     1141 acgagagcca cgtgaccgtg ccgcagatcg gcgccatgtt ccgcggcgac tacaaacgca
     1201 agtccacgct cgccgagtat ggcttccgcc tcccctcctg catggacaac cggcccttgc
     1261 gcttcgagga gtgggacgcc atgcggccgc aaaccgtcta cgtgtcggca acgccgggcg
     1321 gatgggagct ggagcagacc ggcggcgtct tcgccgaaca ggtgatccgg ccgaccgggc
     1381 tcgtggaccc gccggtcgag gtgcgtccgg ccacaacgca agtggacgac ctcatcgacg
     1441 agacccgtaa agtcgtggac gccggctatc gcgtgctcgt gacgacgctc accaaacgca
     1501 tggccgagga cctgacggag tacatgcacg aacagggcat ccgcgtgcgc tacatgcatt
     1561 ccgacgtgga aaccctggag cgcatcgaga tcatccgcga tttgcggctg ggcgccttcg
     1621 acgtgctcgt gggcatcaac ctgctgcgtg aggggctcga cattcccgaa tgcgcactgg
     1681 tcgccattct cgatgccgac aaggagggct ttctccgctc ggagacctcg ctggtgcaga
     1741 ccattggccg cgcggcgcgc aatgtcgatg gccgcgtcat tctctacgcc gacggcatca
     1801 ccgggtcgat ggaacgcgcc atggcggaaa ccgaccgccg ccgcgagaag caagtggcct
     1861 ggaacaaggc gaatggcatc acgccggaaa gcgtgaagaa gaacatcggc gacattctcg
     1921 cctccgtcta cgagcgcgac catgtgctgg tcgacgccgg caccggcttc agcgaggaag
     1981 atcagatgcc catcgggcat aaccttgaaa gcgtgatcgc cgacatggag aagcgcatgc
     2041 gcgcatcggc ggccgatctc gaattcgagg aagccgcgcg cttacgcgac gaggtcaagc
     2101 gcctgcgcca aaccgaactc gccatcgcca acgacccact cgcacgccag tcggaggtcg
     2161 agcgttcggc cgcagcggca gacatctctc gaccgcacaa gccggacctc gacgagatgg
     2221 gcccgcacaa ccgcgagatc ccgctcaaga ccgcgcgccc acgctcaaag tcgggcaagc
     2281 ccggcacacg tgccttcaag ggcaagggcc attaagccgg agcctggccg ctcattcttg
     2341 ctgtacgcga gcctccgacg cctcgttcta aaacggggcg cgcattaacc gtttctcaag
     2401 cctatccggc gaaattaagc gctgtaccga gttggtattc agtagcgtaa agagaatcag
     2461 tcatgcgcat catcattgct cttgcggtct tcaccgccac catcgccttg ggcggttgct
     2521 tccatcacca ccagcagacc tatgtcaccg agcttccgcc ggctccggtg ccctataagt
     2581 aagccttcca gcatgcgccg gtgacgcggc agccctgtcc gctgggaagc ggacgtgccg
     2641 gcagcgccgt tggaaagcga tgctcctgca tcgaaaagcc cggcctagcg ccgggctttt
     2701 tcgcatcacc cccgctcaag cgcttgtgag cagatatgag ccccgagtga cggcaacctc
     2761 ggctagcgtg acaccatcac gccgctcaca gagggaggga gccgaaatgt ctgcactcat
     2821 cgattggctg gaagccggac gccgacttgc cgagcgcatt ccgtatagca tcgtcgcgct
     2881 ggtcgcgcgg ctcgcggtag caaacgtctt ctggcgttcc gctcaaacca aaatcaacgg
     2941 cgtcttcgag gtcaaagact cgacgttcta tctcttcagc gaaaaatacc acgtgccgat
     3001 tctaccgccg gatgtcgcgg cttacatagc gacctaccaa gaactcgtcg gctcggtcct
     3061 attggtcgtc ggcctggcaa cccgcctcac ggcgttggcc tttatcggca tggtcgcggt
     3121 gatccagatc ttcgtctatc cgtcaggctg gcccgaccat attctgtggt tcgcgctgct
     3181 gttcttgctc gttgcgcgcg ggcccggtgc gatctcgctc gaccacctga tctggcggcg
     3241 caccaagtag gcgcagccag ccgccaacag acaggccggt ggaccttatc cgccggcctg
     3301 caacttccga atcaacgctt ggctcggata ggagtcggcc ggcaacccat acttgacctg
     3361 catgtcgcgg atcgccgcgc gggtgccggc gccaatcaca ccgtcgatct tgcccacgtc
     3421 gtaaccgcgc tgctgcagaa gccgctgaag ttgcttgttc tgggctgccg agaataccgg
     3481 cgcattgccg cgacgcagcg gcggcgcgcc gccaaggcgc gtggcgaagt acccggccgt
     3541 cgtcgaatag atgagcgagt tgttccagtc gagatagatg ttgatgaagt tcggataggc
     3601 caggaaagcc ggtccgttcc gccccatcgg caataggagc gccgagggca cgttgtccgc
     3661 gggcaccggc ttaccgttgc ggtaggtgac cccgtaacgc gaccagaaca ggcgcggccg
     3721 ctgaatagtg acgtccgcct gatcccaagg gaggttcttg ggcacaatga cctcttcgag
     3781 ccagggctgc cccggctgcc agcccttcgc ccggatgtaa ttcgcggccg atgccagcga
     3841 gtcccctgca ctgcgcacca aatcgcggcg cccgtcgcca tcgtagtcga cggcatactt
     3901 gtagtaggtg ctgggctgga actggaactg tcccacctca ccatgggcgg gtccgcgcat
     3961 ttgcgacggc gtcaagtcac cgcgatcgat gacgcgcagc gcatccatga gttgaacgcg
     4021 aaattcttcc ggcctgcggc agtcgtagct cagcgtggcc agggagcgaa tgctctgcat
     4081 gtcgcccatg aaggcgccga aatctgtctc cagaccccaa aaggcgacga gcacgggccc
     4141 tggcacgccg tattgctgtt cgacttgctt gagcgttttc ccgtatttcc gcatcctggc
     4201 cttgccagct tgcaggcgat tcttggacac catgcgcctg gagaacgtgt tgaagtcctg
     4261 cgcgaagaca ccttgcgcgc ggtccttctt gatggtcctc ggatcgtagg tgacgccgtt
     4321 cagcgcggcg gccacggtct gcggcgagat accttcggca atcgcctgct tcttgaaccc
     4381 gtgcagccac ttgttgaagc ccgaaggacc ggggcatcgc tgcgcaaagg cggtggaaac
     4441 cgagccgagc agcaccgccg cacagaggcc agcgagcgcc gacgtcgaaa tgatgcgtgt
     4501 cagggaatat ctagccatgc gcgccaatga acccagtcgc ggcgcgtccg tcaagccaag
     4561 aggatgtcat ctttgcagtc taaccagtgc gtcgccgaag ccgccgcaac tgggaaaggc
     4621 ctcaatcggt gtgcggcggc aactcgccga tccgaagctt ctctgtcagc acgttgatgc
     4681 gccgagaagc ctcagccttg ctcagtcctt gccggtacgc ctccggttcc cgcacttcct
     4741 cagccaaatt cttgaggtgc gaggcctgtg cccccgtcat cggctcggct ccagtcgatc
     4801 cactgggcgc gagttcttgg cgctcctggt aagcggaggc cgtcttcttc tgttgcatcg
     4861 tagtcttcct gaaccgtctt tggtgtttcc cttgaggttt gtgttcttca tacgattgtt
     4921 acagtaggaa tcgaaaaggg caagcatttg gcgccatttg gcaaaggttt gggcgcgttt
     4981 cgtgacccgc ccaaagatca ggcgtcggtc aagcatctga acaacatggt ttggcggcct
     5041 ttggccacta tgtagcgatc cagcaatcgc cccttgccca taggcggcgc cggccctccc
     5101 aggcgccgct aatctcttgt aaagattgta gaaacggtca cgaccggccc cgaaatggcc
     5161 tctctaaggc cgtgggcgag ccgatctttt gcccttttgt tccatagatt gatgcgctgc
     5221 acaaaaagtg ctgccggcgc gattgtcacc ggctctcaag caaaaaacct cccaagaatt
     5281 ggaacaaagg agccaatacg catggtcaaa gtgcgagcga agtcgattgc cgcgggtctt
     5341 ttgggggccg ccttgatggt gactgtgagt gccgcgatgg cggaccaggt cggaagtgcc
     5401 tcctggtacg cgctgacctc caaaacggcc agcggcgagc ggatgaaccc gaatgcactg
     5461 acggcggcac atcggtcgct gccattcggc tcgaaagtgc tggtggaaaa tctccgcaac
     5521 ggccgatcgg tggtcgtgcg gatcaacgac cgcggcccgt tcaccggcgg ccgaattatc
     5581 gacctgtcga aggcggcagc cgctcagatc ggcatgatca actcagggac cgccaaagtc
     5641 cgcatgaagc ggatcaaata ggccttctgg aacactattc cattgaaata acttgtgttt
     5701 ctccatatcg tgcgagatcc agagccgcga gacgccgggc ctttgcgccc ggcgtctctg
     5761 ttgtccgccc gcaaaactta atgtctattg ttgcatagat gcgctaggca gtggctgcca
     5821 aaggcggcat agtcggccca tcggagcctg aatgccgact ctaaggtgcg ggcgcggcaa
     5881 cgccgtcgtg atcgatgaaa tgctaagatt cgcaggggct tagatccgcc ctatccaacg
     5941 gagccatgac gcccatgctg acccgccccg cccttcggac gattctcccc tacgcggcat
     6001 tcctgatcgt cgcgtctttg accttcgtgg cgttcgatac cgctgtggcg cagcaacagc
     6061 agtcgcagcg cgggtccaac caaggcctgt tcaagaagcc ccatcgcggc ccggccatcg
     6121 ggggcacgtc ttggaccctt agcgacgtct ggggcgaacc ccgaatgcca gagccgtctt
     6181 acgattaccc cgggttcaat tattcgcctg gcggctatac gctcgacggc gcgccgaaca
     6241 ctgcgcccta ccccaactaa acacccgttt cacttacgtg agcgcgcaat gggccttgcg
     6301 cagaactgcc tgacgcccct agagtgaggc gctttcaggg cagactgcga acagggtgga
     6361 gactggccgc gtacgctcct gcgacgtggt ttcttttccc gtttcttaga cttgcctgat
     6421 tttccgcgcc tgcgcgcgga ccaattggag cgggaaagga gcttcccatg cgtcagaccg
     6481 gtacggtcaa attcttcaat cattcgagag ggttcggctt tatcacgccg gacgatggcg
     6541 gcaaggatgt gtttctacac atctccgcgt tgcagcagtc ggggcttccg gcactcgacg
     6601 agggggcaaa ggtctcattt gaaacagagc cggatcgccg cggcaaaggc ccccaggcga
     6661 tcaacgtaca aatgggcgaa taatctatct gcacagttcg agagttcccc gctgcgatag
     6721 tggcatcatt cgacgccgct atcgtcagcg ggattttttt ggtcgcgcgc cgctgaacgg
     6781 cagaccaaga caccttgcct cgagacacgt tagcttatga cggtggaccg aacccgcgcc
     6841 tcagaccaac ttcgcagcgt gctcgaccgt ctcaccgagg gacgcgcggc cgcactggag
     6901 cggctgttcg actttttggc gattcccagc atctccaccg accccgcctg tcacgaagcc
     6961 tgtctttatg cggccgactg gtgcgccaag agcttgtgcg acatcggctt cgaggcgcgc
     7021 gtcgaaccga ctgacggcaa gccgatggtc gtcgcgcatt ggcgccatcc ggacggcggc
     7081 gctcgccctc acgttctttt ctacggtcac tacgacgtgc agcctcccga cccgctggag
     7141 gcttgggatg cgcccccctt cgaggcgcag ctcgtcgacg acgcgcagca tgggcaagtg
     7201 atcgtggcgc gcggcgcgtc cgacgacaaa ggccaggtga tgaccttcct tgaggcctgc
     7261 cgcgcctgga tggccgtgga cggcgcgctc cccgtcgacg tgacggtgct gttcgaaggc
     7321 gaggaggagt cggggagtcc cagcctcgcg ccctttcttg cagcccatgg cgatgaactg
     7381 caggccgata tcgcccttgt ctgcgatacg ggccaatggg acccggagac gcccgcgatc
     7441 accgccttcc tgcgcgggct tgccttttcg gaaatcaccg tcagcggtcc atcgcgcgat
     7501 ctgcattcag gcatttacgg cggtccggcc cgcaacccca ttcgcgtcct cgccgaactc
     7561 gtcgccgact tacatgacgc acaggggcgc gtgaccattc ccggcttcta cgacgatgtg
     7621 cacgaaccct cgggcaagca gcgcgacgcc tggggggcgc ttggcttcga cacgtccgcg
     7681 ttcctcggcg aaattggtct gagcgagcct gcgggggaaa cggacagaaa cgtgctggaa
     7741 cagctctggt cgcggccgac ggtcgaggtc aatggcatga tcggcggcta tacgggaccg
     7801 ggcaccaaga cggtgatccc cgcacaagcc agcgcgaagc tttcgtttcg cctggtgccg
     7861 aatcaagacc ctgccaagat catcgacggg ctccatcgct tcatcgcgga acgcctcccg
     7921 ggcgacgcgc gcgtttcctt cggcggcgaa ggcggctcgc ccgcggtcgg cttcgacacg
     7981 ggcgcgcccg cgttccgcgc cacagccgcc gcgcttcaag acgaatgggg caaaccgccc
     8041 gtggtcgtcg gctgcggcgc ctctattccc atcgtcgagt cgttccgcac gcggctcggc
     8101 atggatgcgc tgttggtcgg attcgctctc gacgacgacc gcattcattc gccgaacgag
     8161 aagtacaacc tcacgagctt cgagaagggc gcgcgaagct gggcgcgcat ccttgcaaag
     8221 ctgggggatc acgatgtctc gctttgacgg gtgcatcacg ggtgttggcg ctgccgctat
     8281 cgccgccaca ttgctctgcg cgtcggccgg ggtcgcctat gacgacaggc ctcacgccta
     8341 tcgtcacgcc gagcccggca atatcgacta ttacgcgctg gtgttgagct ggtcgccaac
     8401 gcattgcctc atggaaggcg gtccccgcgg cgatcagcaa tgcgatgcga aacagccgga
     8461 cgacttcgtg ctccacggct tctggccgca atacgcaaag ggctggccgg aagattgcta
     8521 caccggccag cgcccttggg tgccatcagc cgtgatcaac gagatgctgg gcgtcatgcc
     8581 aagtaagggc atgatcatcc acgagtacag gacgcacgga acctgctccg gcctgagccc
     8641 ggccgactat ttcgccgccg cccgcattgc ccgtgaccag gtccacattc ctgcgatttt
     8701 cgacgacccg aagacgcaac acttcttgtc gcccgaaaaa atcgagagcg aatttatcgc
     8761 gaccaatgat tggctgacgc ctgacatgat cgcggtgacg tgccggcgtg gaaaactgtt
     8821 cgacgtccgc atctgcttcg gcacagacct tcagccacgt gcgtgcggct cgaacttcaa
     8881 tcagaagcgc ttgtgtccgt tgcgccgggt taccgtaacg gtcccttaga agcggtagat
     8941 caccgagccg cggaacgtgt ccgaatccgg atcgaaggtc gccttgccac cgggatagga
     9001 gactgtctcg gcgtcgaagt cagtaaactg atagtcgaag cgcccgctcc agttctggtc
     9061 gaacgcatac tccgcgccac cgccgacgct ccaaccgaag aacgtcgctt cgcggaaagc
     9121 accgccggga ccgctgatcg gaagatccgc gtcggcccag gaaccgccga aggttccgaa
     9181 caggagcagg ttgggcagaa ccgtatagcc cgcacgcaaa cgcagatcgg cgaccgagtc
     9241 gaacccgggg ctcaccttgt tgagaccgcc gccaaatctg cggctgccgc tcacatcgga
     9301 agcgagaaaa tcgccttcga tgcccacgac gaagggaccg tgctgccagc tatagccgcc
     9361 gatgatacca cccacggcgc cgtcggacga caccgagccg gccggcgtct tgtcgtagct
     9421 cactgaggca ccgccgatcg tgccgccgag ccaccacccc tcgaaaagat cttgggacgg
     9481 gaccggcgcc atgtcgtaaa cgggcgctgt gtcgtagacg tcgccaccac ttaatgcggg
     9541 gcttgaggcc gtggcgctgg ccaccagcac gacggcgcca gtcgcggccc gatacaatcg
     9601 cgtacgcatg acaaactccc tcacgattgg aggctcggtt ccgttcgcca ggtggcccga
     9661 accaagcgct cacggcctca agggtagtgc cctaccctta ccgaccgtta acgacgaacc
     9721 ggggcgaaac tgggtagggt tagcggcggg ttaatggcca tcccgagacg gactttggcc
     9781 tgcaatgacc ctgcgaggct cagcgcgccg tgaacaaggc cacaaggccg tcctgctcga
     9841 tcctcacggt agcgaaacgc tgccggagcc cgtcggtcag agcctcgatg ccgtcggcgc
     9901 cgttattgaa cacgcccttc ttgttgtaga gatcgagcaa accccgcgcc gcgatgttgg
     9961 gcacaacccc acggccaaga atcgtggcgc cgaacagcct cgccccctcc gccatgaacg
    10021 gcttgaggtg gtcgaccgcc accagcttct cagacatggt gcccggcagg cagtgcagca
    10081 cataggtcag gcccaccgag gtgaatggcg cgagatcgaa ctccaggggc gccaacagat
    10141 tggcttggcg caatgccggc cggaaccgcg ccagccggcg ccccgccaaa tcaaggcaat
    10201 gcacgttgat gtcggccaag accagccggt cgaaacccgt gccggcgcgg tcgagaaaca
    10261 gccccgtgcc cacccccgct tccaggtgat tggctggcag gttccgcgcg tagagttcgt
    10321 cgagccgcga ggtcggacac cgccaggcaa accggttgga cagcttatgc accacgagat
    10381 cgtagaaacg gagcgcggca ggcgtgtaga ccgcgtgggc ctta
//
LOCUS       DBNHVU010000094        13440 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_184883, whole genome shotgun sequence.
ACCESSION   DBNHVU010000094 DBNHVU010000000
VERSION     DBNHVU010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 13440)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13440)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13440
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_184883"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..2137
                     /locus_tag="AC16Y7_03555"
     CDS             356..2137
                     /locus_tag="AC16Y7_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR5708490.1"
                     /translation="MEDTPEDEAKRPSLRPLLRLRPYLLRHKGMLAAAFVALVAAAAA
                     TLAVPLAVRRMIDLGFSGIEPDLIDTYFMTLVGVGVVLALASAARFYCVNWLGERVVA
                     DIRSDVFKHLTGLSPAFFEVSHSGEVMSRLTADTTQVKAAASTAISQAIRNVLLLAGA
                     VVMMVVTSTKLSLAVLIAIPIIVLPLVAYGRSVRARSRLAQDALAHASAYASESLGQV
                     KVLQAFTHETATTERYRDAVDRSFEAANARTKARAGLTAIAIFLVFISVVGVLWYGAQ
                     DVLSGAITGGTLGQFVLYAVFAAAAVGSLSEVWGEVAQAAGAAERLGELLQVRSQITS
                     PEHPKAMPEPPDGKIAFDDVSFTYPLRPEQRALHDVSFQVKPGERVALVGPSGAGKTT
                     IFALLLRFYDPESGAVRIDGVPVNEASLSDLRARFALVPQEPALFADTVAANIAYGAG
                     KASRAKIEAAAKAAHAHEFIMELPQRYDTELGEGGVTLSAGQRQRIAIARAVLRDAPI
                     LLLDEATSALDARSETLVQKALDKVMEGRTTLVIAHRLATVVNADRILVFDRGRLVEQ
                     GTHQVLMREGGVYARLADLQFAPDAAE"
     gene            complement(2139..3167)
                     /locus_tag="AC16Y7_03560"
     CDS             complement(2139..3167)
                     /locus_tag="AC16Y7_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HIR5708491.1"
                     /translation="MALARARRRQYTPNYWPGFVDMLSTLLLVVIFLMAMFMVTNYII
                     TQAASGKDTMLSRLTRQLSELTELLALERSKKESAEDNLSALQATLLNTESENKRLTG
                     MLGTASGATEDAEGRIAALGTKLDDQRKITNDALAKVELLNQQLAALRLQLAALNEAL
                     EASEAKDKDAQAKIADLGQRLNVALAKKVKELNEIRSDFFGKLRAALGARQDFKIVGD
                     RFVFPSDVLFDPGSSDLKPGAAPELNKLVTALKEIEGNIPPDIAWVMRVDGHTDINPI
                     ATPEFPSNWELSSARAISVVRYLMSQGISPQHLVAAGFGEFQPIDAGSSDEALARNRR
                     IELKLTER"
     gene            complement(3170..4192)
                     /locus_tag="AC16Y7_03565"
     CDS             complement(3170..4192)
                     /locus_tag="AC16Y7_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HIR5708492.1"
                     /translation="MATKQYKTKLTRPQVFLWRMMLFLVLAVLLAIILDGQTEQLRRS
                     FMANPGLNGLIIAVLVVGIVYAFRQVLRLYPEINWVNNFRISDPGIATDKQPVLLLPM
                     ATMLRDRTGHLALATGSARTLLESVASRLDEARETTRYLVGLLVFLGLLGTFWGLLQT
                     VSSVGNTINALDTTGSESVLLFDQLKEGLAGPLSGMGIAFSSSLFGLAGSLILGFLDL
                     QAGHAQSRFYNELEDWLANITELQSVDPGTKDVSPQLRFALMDMQHSLSDLNEKVEQR
                     ILNDNTGDAVKDLAQGVDKLIRQMRAEQKVVREWADEQAQQQHELATVLRQISGKKPA
                     PAREGN"
     gene            complement(4346..5212)
                     /locus_tag="AC16Y7_03570"
     CDS             complement(4346..5212)
                     /locus_tag="AC16Y7_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007007083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR5708493.1"
                     /translation="MPASALMNVMQTAARKAGRSLTRDFGEVEQLQVSMKGPANFVSA
                     ADHRAEEILFTELSRARPGYGFLMEERGEVKGADATHRWIVDPLDGTTNFLHGLPLFA
                     ISIALERDGELVAGLIYNPVSDETFTAEKGKGAYLNDRRRLRVAARMELSASLICTGS
                     PHAGRPNHDLYLRELRAIMAVSSGIRRTGSAALDLAWTAAGRYDGFWESGIKPWDIAA
                     GIVMLREAGAYATDIAGKDKMLETGSIVAGNETIHAKLLKLLRAADARGPAPVKAAGK
                     TASKAPAKTAAK"
     gene            complement(5249..5812)
                     /gene="efp"
                     /locus_tag="AC16Y7_03575"
     CDS             complement(5249..5812)
                     /gene="efp"
                     /locus_tag="AC16Y7_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR5708494.1"
                     /translation="MKINGNEIRPGNVITHKGGLWVAVKTQAVKPGKGPAYAQVELKN
                     LIDGSKLNERFRSSETVERVRLEQKDHQYLYPEGDMLTFMDLESYDQIQIPSDLLEER
                     AAFLQDGMTVTVESHEGRPIGVSLPDHVTLEVVEADPVVKGQTAASSYKPAKLENGVR
                     VMVPPFVEAGERIVVDTNEVTYVKRAE"
     gene            complement(5873..6847)
                     /locus_tag="AC16Y7_03580"
     CDS             complement(5873..6847)
                     /locus_tag="AC16Y7_03580"
                     /inference="COORDINATES: protein motif:HMM:NF019843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5708495.1"
                     /translation="MRRLFAGVLIASACILASGPARAAESPSYIAFAEGKYLTALDLA
                     QKEAEAGSKEAYTLMGEIHAEGLGVPQDYAKAADAYAKGADLGDPNAQFSLGLLVASG
                     RGVRKDLTIAGDLFERAAKAGNAAAQYNVALLYLHGRGRPADEIKAAEWMQKAATHGS
                     PQAQYDLGAFYQFGRGVPIDKQKAAEWTGRAADAGLPAAQVEYGVMLFNGRGVEKDEK
                     RAATLFRQAAENGNPVAQNRLARLYANGVVFEGDAIQAAKWHLIARQAGVSDFTLDIM
                     LSRLAPEQRVEAERLVDGWEAGRLTDDASDGQAAVDTQPAGQQAPSRP"
     gene            complement(6844..7458)
                     /locus_tag="AC16Y7_03585"
     CDS             complement(6844..7458)
                     /locus_tag="AC16Y7_03585"
                     /inference="COORDINATES: protein motif:HMM:NF014622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR5708496.1"
                     /translation="MADAKSQCRLYFQVPAPITAKLESQLAQALTTTSVACVVLCDNS
                     QNTEEVPTDEPSDRLIDRIQGANIACLIENDFERAERLGADGVHIAADLERYTTARNL
                     LGQSANIGVDCGADRHAAMELAEAGADYVAFGPESEGLVAWWSEVFVVPCVAWNVDTP
                     AEAERFAQQGADFVAPSRTIWQADNAATIVADIGRAIAHVRSTA"
     gene            7687..7953
                     /locus_tag="AC16Y7_03590"
     CDS             7687..7953
                     /locus_tag="AC16Y7_03590"
                     /inference="COORDINATES: protein motif:HMM:NF025122.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4164 family protein"
                     /protein_id="HIR5708497.1"
                     /translation="MKQPDALHRLDTAVDQLEGHLKQAFAGGGDAALKDELRFLTEER
                     DQLLADLEAERSRVRRLKAANDEVSGRLEVVMGTLKDMIPAMPG"
     gene            7976..8356
                     /locus_tag="AC16Y7_03595"
     CDS             7976..8356
                     /locus_tag="AC16Y7_03595"
                     /inference="COORDINATES: protein motif:HMM:NF017016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR5708498.1"
                     /translation="MGQISVTLNGRNYRLECSEGEEAHLLELAEYLGSHVDEMRRKFG
                     QVGDDRLILMASLVVTDELWELRREIENMKTALAEARRDKSASDETAKHMQADLSARI
                     DAAAERLEGLNAELSKGGRASDGR"
     gene            8391..8548
                     /gene="ssrS"
                     /locus_tag="AC16Y7_03600"
     ncRNA           8391..8548
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="AC16Y7_03600"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            8618..9448
                     /locus_tag="AC16Y7_03605"
     CDS             8618..9448
                     /locus_tag="AC16Y7_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009759622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HIR5708499.1"
                     /translation="MRILFLGDVVGRSGRAAVLETLPKLRERYRADFVVVNGENSAGG
                     FGISEAIFNELLDAGADVVTTGNHVWDQREALVFIERHDRLLRPVNFPAGTPGRGAGL
                     FKAANGMDVLVVNAQGRVFMADLDDPFRAVEQELSACGLKSGTDAILIDFHAEATSEK
                     QAMAHFVDGRATAVIGTHTHVPTADEFVLNGGTAYISDAGMCGDFDSILGMDKEEPIA
                     RFLTKIHSRRFAPSMGEATVCGVAIEVDDATGLAKAIAPVRLGGRLSQVEPAFWLDPA
                     "
     gene            complement(9445..9675)
                     /locus_tag="AC16Y7_03610"
     CDS             complement(9445..9675)
                     /locus_tag="AC16Y7_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708500.1"
                     /translation="MHRPATGEAAEQTLSLPDLCSEAADHIARARLSLDDKGYDTDRA
                     LAHLDDAIGCLQRLLAKGREPHATATPSRRSA"
     gene            9854..10603
                     /locus_tag="AC16Y7_03615"
     CDS             9854..10603
                     /locus_tag="AC16Y7_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR5708501.1"
                     /translation="MAGHSKFKNIQFRKGAQDKKRAKLFSKLAREITTAAKLGMPDPA
                     MNPRLRTAIQAARVQNMPRDNIDRAIKKSQEAGGDNYEEVRYEGFGVSGVGVIVEALT
                     DNRNRTASEVRSIFSKHGGNLGETGAVSFNFDRLGAIEFEAGKGSADDMLEAAIEAGA
                     DDVETTDEGHVVYCNPDELHQVGKALEERFGETRAMQILWRPKIEVPIDEEAGEKLLR
                     MIDALEESDDVQQVYANFDVSDDVLQKLTAA"
     gene            10621..11043
                     /locus_tag="AC16Y7_03620"
     CDS             10621..11043
                     /locus_tag="AC16Y7_03620"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HIR5708502.1"
                     /translation="MTQQEPAEYKHLREQSALPGKGGLARIGRAFANTLGGFREGLAT
                     EAAIKQEAALTLFALPVSFFVADSAWMWIALMGSLVLLLAAELLNTAIERLCNHLHPE
                     KHEAIKVTKDLASAGVFCVIVLTAMIWIVAVVVRVWAG"
     gene            complement(11040..11612)
                     /locus_tag="AC16Y7_03625"
     CDS             complement(11040..11612)
                     /locus_tag="AC16Y7_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708503.1"
                     /translation="MAKAAPIWLRLLLGALLLPLIWVGLALLLPSALSSAAVDFVDDH
                     VRAELRAALGGSLLVLPVLVGGARMPRPEQLPEDIKAIATLNAVPLRHESFDDDTENI
                     VATVLGVAAKTRRWEDRGAWKAKIAYALGGAAAACMFVLVAALIHFWILSRPLSASIG
                     APLTTLLLISGMASGAWLGLSFEARKRKLR"
     gene            11723..12574
                     /locus_tag="AC16Y7_03630"
     CDS             11723..12574
                     /locus_tag="AC16Y7_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5708504.1"
                     /translation="MVEDGAGTWSLRDSERVIAGNAALLGRTHQLVAMTRVRNEALIL
                     PDTLDYLSSHVDAIIAYDDASTDETVDMLRAHPKVALIVANQAWERDVDARKRAEGRH
                     RGLLLEMARAQVPHDWMFCFDPDERVTGDVRRHLYGLDADCDALRVRLFDAYLTPEDQ
                     APYTSDQELLGFRKYYGPEQRDILMFWRNREGIGFAQGDGRTPRGMTAVKTDLYCQHY
                     GKSLSVAHWEETCDYYVQHFPYETYGAKWEARKGKAVHIESDFGNPLHEWGEALFAQA
                     VAMPSQK"
     gene            complement(12674..13342)
                     /locus_tag="AC16Y7_03635"
     CDS             complement(12674..13342)
                     /locus_tag="AC16Y7_03635"
                     /inference="COORDINATES: protein motif:HMM:NF019430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIR5708505.1"
                     /translation="MNISKRIASIAIVAVLSVAGLAAVTMAGGHDGRWGQSAWAGKHG
                     DKSMCHRGGGGKHGRHGWRHKGPDHLAKKLSVMETEIGIRANQLDAWRDFTDALQATM
                     KRPMRHGGTGMMASGDNAVPFSLAERFANNAIERGESAEALKKAIAELRTTLTPEQLQ
                     KVTEIEKRFRAKMARHHGWGKGHRGKGRHGKGPHGPPKAAPDGGSDTGPGGTPDKDDD
                     DDDS"
BASE COUNT         2329 a         4092 c         4425 g         2594 t
ORIGIN      
        1 ccgtcatgac caccttgatc tggtggtact ccgggtgaat gtcttttttc atggctcaaa
       61 ccttcattgt ccggcgccgc ccgatcgtcc ttgccgaggg caccccacgt cgcctgatcg
      121 aattctgcgg aattggcgtg agcctataac gcggaaccgg tgctagggca agatcgcgtg
      181 caaacggggg ccgttcccgg ggcttaacct tgcgggagac agtgccggcg ggcgcggcat
      241 caaggccttt gcgcttcccg ctcaggccct taaagtcggc caactgcatt ctagctgggc
      301 atatctagtg caagtcgtat cgaactccga cggtcaagat cggcaacacc ctgagttgga
      361 agacaccccg gaggatgagg caaagcggcc ctcgctgcgc ccgcttctga ggctgcggcc
      421 gtatttgctg cgccataagg ggatgctcgc cgccgctttc gtcgcgctgg tggccgccgc
      481 cgcggccacg ctcgccgtgc cgttggccgt gcggcgcatg atcgatcttg gcttctcagg
      541 catcgagccc gacctgatcg acacctattt catgacgctg gtcggcgtgg gtgtggtgct
      601 ggcgctagcg agcgccgccc ggttctactg cgtgaattgg ctcggcgagc gtgtggtcgc
      661 cgatatccgc agcgatgtgt tcaaacatct gacgggtttg agcccggcct tctttgaggt
      721 ctcgcactcg ggcgaagtca tgtcgcggct caccgccgac accacgcaag tgaaggcggc
      781 cgcgagcacg gcgatcagcc aagccatccg caacgtgctg ctgcttgccg gcgccgtggt
      841 catgatggtg gtgaccagca ccaagctgtc gctcgccgtg ctgatcgcca ttccgatcat
      901 tgtgctgccg ctcgtcgctt atggccggtc cgtgcgggcg cgttcgcggc tcgcgcaaga
      961 tgcgctggcg cacgcgtcgg cctatgccag cgagagcctc ggccaggtga aagtgttgca
     1021 ggccttcacc catgagacgg cgaccacgga acgttaccgg gacgccgtgg accggtcctt
     1081 cgaggccgcg aatgcgcgca ccaaggcgcg tgccggcctc accgccattg ccatctttct
     1141 cgtgttcatc agcgtcgtcg gcgtgttgtg gtacggcgcg caggatgtgt tgtccggcgc
     1201 cataaccggc ggcacgctcg ggcagttcgt gctctacgcc gtattcgccg ccgcggccgt
     1261 cggcagcctc agcgaggtgt ggggcgaagt tgcgcaagcc gcgggcgcgg ccgaacggct
     1321 tggcgagttg ctgcaagtgc gctcgcagat cacgtcgccc gaacatccca aggcgatgcc
     1381 tgagccgccc gatggcaaga tcgcatttga cgacgtgtcg ttcacctatc cgttgcggcc
     1441 cgagcagcgc gcgctccatg atgtgagctt ccaggtgaag ccgggcgaac gcgtggcgct
     1501 tgtcggccca tcgggtgccg gcaagacaac aatctttgcg ctgctgttgc gcttctacga
     1561 tccggagtct ggcgccgtgc ggatcgacgg cgtgcccgtg aacgaggcca gcctttcgga
     1621 cttgcgcgcg cgtttcgcgc tcgtgcccca agagccggcg ctgtttgccg acacggtggc
     1681 cgccaatatc gcttacggcg ccggcaaagc gagccgggcc aaaatcgagg cggcggcgaa
     1741 agcggcgcac gcccacgagt tcattatgga gttgccgcaa cgctatgaca ccgagctggg
     1801 cgagggcggt gttacgctct cggcggggca gcggcagcgc atcgccattg cgcgcgcggt
     1861 cttacgcgat gcgccgatcc ttctgctgga cgaggcgacg agcgcgctcg atgccaggag
     1921 cgagacctta gtgcagaagg ccttggacaa agtgatggag gggcgcacca cgcttgtcat
     1981 cgcgcaccgg ttagcgaccg tcgtgaacgc cgaccgcatc ttggtgttcg atcgcggacg
     2041 tttggtggag caggggaccc atcaagtcct gatgcgcgaa ggcggcgtct atgcgcggct
     2101 cgccgacctg caattcgcgc ccgacgccgc agagtagcct agcgctccgt cagcttcagt
     2161 tcgatgcggc ggttgcgggc cagcgcctcg tcgcttgagc ctgcatcgat gggctggaac
     2221 tcgccgaacc cggcggccac gagatgttgc ggcgaaatcc cctggctcat gagatagcgc
     2281 accacggaga tggcgcgcgc gctcgacagc tcccagttgg agggaaattc gggcgtggcg
     2341 atcgggttga tgtccgtgtg gccgtcgacc cgcatgaccc aggcgatgtc cggcgggatg
     2401 ttgccttcga tctctttcag tgccgtgacc agcttgttga gctcaggcgc cgcgccgggc
     2461 ttcagatcgg acgagccggg atcgaacaga acgtccgagg ggaacacgaa gcggtcgccg
     2521 acaatcttaa agtcctgcct tgcccctaag gctgcccgca gcttgccgaa gaagtcggag
     2581 cggatctcgt tgagctcctt caccttcttg gccagggcta cgttcagacg ctggccgaga
     2641 tcggcaatct tggcttgggc gtctttgtcc ttcgcctctg aagcttcgag cgcctcgttc
     2701 aaggcggcga gctgaagcct gagcgcggca agctgctggt tgagcagttc gaccttggcg
     2761 agcgcgtcat tggtgatctt gcgctgatcg tcgagcttgg tgccgagcgc ggcaatgcgg
     2821 ccctcggcgt cttccgtcgc accgctcgcg gtgccgagca tccccgtcag ccgcttgttt
     2881 tccgattcgg tgttcaggag cgtggcttgc agcgccgaca gattgtcttc ggccgactct
     2941 ttcttggacc gttcgagcgc cagcagttcg gtcagctcgg agagctgccg ggtcagccgc
     3001 gacaacatcg tgtccttgcc gctcgcggcc tgggtgatga tgtagttggt gaccatgaac
     3061 atggccatga ggaagatcac cacgagcagc agcgtggaca gcatgtccac gaagcccggc
     3121 cagtaattgg gggtgtattg acggcggcgg gcgcgtgcca gggccatggc taattgcctt
     3181 cgcgggccgg cgcgggcttc ttgccggaga tctggcgcag caccgtggca agctcatgct
     3241 gttgctgcgc ttgttcgtcg gcccactcgc gcacgacctt ctgctccgcg cgcatttggc
     3301 gaatgagctt gtcgacgcct tgcgccaagt ctttcaccgc atcgcccgta ttgtcgttga
     3361 ggatacgctg ctcgaccttc tcgttgaggt cggacagcga gtgctgcatg tccatcagcg
     3421 caaagcgcaa ttgcggcgag acatccttgg tgccgggatc caccgactga agctcggtga
     3481 tgttggcgag ccaatcttca agctcgttat agaatcggct ttgagcgtgg ccggcctgca
     3541 gatcgaggaa gccgaggatc agcgagcccg ccaggccgaa cagcgagctg gagaaggcaa
     3601 tgcccatgcc gctcaacggt ccggccaagc cctctttcaa ttgatcgaag agcagcacgc
     3661 tttcgctgcc ggtcgtgtcg agcgcgttga tcgtgttgcc gaccgagctc acggtctgga
     3721 gcaggcccca gaacgttccg agcagtccaa gaaagacgag caagccgaca aggtatcgtg
     3781 tcgtctcgcg cgcttcgtcc aggcgcgagg cgacggactc caggagtgtg cgggctgagc
     3841 cggtcgccaa cgcaaggtgg ccggtgcggt cgcgcagcat ggtcgccatg ggcagcagca
     3901 agacgggctg tttgtcggtt gcgatgccgg ggtccgagat ccggaaattg ttgacccagt
     3961 tgatttccgg atagagccgc aagacctggc ggaaggcata gacgatgccg accaccagca
     4021 cggcgatgat caggccgttc aggcccggat tggccatgaa gctgcgtctc agttgctcgg
     4081 tctggccgtc gaggatgatg gcaagcagca cggcgagaac caggaacagc atcatgcgcc
     4141 agagaaagac ctgcgggcgc gtgagcttcg tcttgtactg tttcgttgcc atgtggtgcc
     4201 gctgctgcgt ggtgtgcttc gcgcgagaga ttagccgaat agtttgaccg gcaaaacggc
     4261 aacctcaggg cgaagacgtt cttatcagcc cgctgtcatg tttgccgggg ccgcggggtc
     4321 gggcgcgggc gcctcgacga tgcgcttatt tcgcagccgt ttttgccggt gccttgcttg
     4381 cggtcttgcc cgcggccttg accggcgcgg gccctcttgc gtccgcggcc ctgagaagtt
     4441 tcaagagctt ggcgtggatc gtctcgttgc cggcgacgat actgcccgtt tccagcatct
     4501 tgtccttgcc ggcgatatcg gtggcatagg cgcccgcctc acgcagcatg acgatgccgg
     4561 cggcaatgtc ccagggcttg atgccgctct cccagaaccc gtcataccgt ccggccgcag
     4621 tccatgccag gtcgagcgcc gcggagcccg tacggcgaat ccccgaggac acggccatga
     4681 tcgcgcgcag ctcccgcaag taaagatcgt ggttcggacg ccccgcatgg ggactaccgg
     4741 tgcagatgag agaggccgaa agctccatgc gcgccgccac tcgcagacgg cgccggtcat
     4801 tgagataggc gcctttgccc ttttcagcgg tgaaagtctc gtcgctgacg ggattataga
     4861 tgagtccggc cacgagctcg ccatcgcgct ccaacgcaat ggagatggcg aagagaggaa
     4921 gtccgtgcag aaaattggtg gtgccgtcga gcggatcgac aatccagcga tgggttgcgt
     4981 cggcgccttt gacctcgccg cgctcctcca tgagaaagcc ataaccgggg cgcgcgcgcg
     5041 acaactcggt gaacaggatt tcctctgcgc ggtggtccgc ggcgctcacg aaattcgccg
     5101 ggcctttcat tgaaacctga agctgctcca cctcgccgaa atcgcgcgtg aggctgcggc
     5161 cggccttgcg ggcggcggtc tgcatgacgt tcatcaacgc ggatgcgggc attgttcgtt
     5221 ctcgccgccg gcgtggccgg ctctttatct attcggcgcg tttgacgtag gtgacttcgt
     5281 tagtgtcgac cacgatgcgc tcgccggcct cgacgaatgg cggcaccatg acccgcacgc
     5341 cgttctccag cttcgcaggc ttgtaggacg acgcggctgt ctgtcccttc accacggggt
     5401 cggcctcgac gacttcgagc gtgacgtggt cgggtaggga gacaccgatg gggcggcctt
     5461 cgtgactttc cacggttacc gtcatcccgt cctgcagaaa cgccgcgcgc tcctccagaa
     5521 ggtcgctggg gatctgaatc tggtcgtagc tctccaagtc catgaaggtg agcatgtcgc
     5581 cttccggata gagatattgg tgatccttct gctccaggcg cacgcgctcg acggtttcgg
     5641 aggagcggaa gcgttcgttg agcttcgagc cgtcgatgag gttcttcagc tcgacctgcg
     5701 cgtaggcggg acctttgcct ggtttcacgg cctgtgtctt caccgcgacc cagaggccgc
     5761 ctttatgcgt gatgacgttg ccgggcctaa tctcgttgcc gttgatcttc atgaaatccc
     5821 ttggggttgg gtaacgggcg tggcgccttt aaggcttggg ggtccgccgc tgtcaagggc
     5881 gggagggcgc ctgctgaccc gccggctgtg tgtccaccgc ggcctgtccg tccgacgcat
     5941 cgtcggtgag gcgtcccgct tcccaaccgt ccaccaaacg ttcggcctcg acgcgctgtt
     6001 cgggggcgag cctcgacagc atgatgtcga gggtgaagtc gctgacgccg gcttgccggg
     6061 cgatcagatg ccatttcgcc gcctgtatgg cgtccccttc gaacaccacg ccgttggcat
     6121 agaggcgtgc cagccggttc tgggcgacgg gattgccgtt ctcggccgcc tggcggaaga
     6181 gcgtggcggc gcgtttttcg tccttctcca cgccgcgccc gttgaacagc atgacgccat
     6241 actcgacctg ggcggcgggc agcccggcgt cggcggctcg gcccgtccat tcggcggcct
     6301 tctgcttgtc gatggggacg ccccggccaa attggtagaa ggcgccgaga tcgtactgcg
     6361 cctgcgggct gccgtgcgtg gcggctttct gcatccactc cgccgccttg atctcgtcgg
     6421 ccggacgtcc tcgcccatgc aggtaaagga gtgcgacgtt gtactgcgcc gcggcattcc
     6481 cggccttcgc ggcgcgctcg aacaggtcgc cggcgatggt gaggtccttg cgcacgccgc
     6541 ggcctgaggc aacgaggagc cctaaggaga actgggcgtt ggggtcgccg agatctgcgc
     6601 ccttggcgta agcatccgcc gccttggcgt agtcttgagg cacgccgaga ccttccgcgt
     6661 gaatctcgcc catcagcgtg taggcttcct ttgagcccgc ttccgcctct ttttgcgcaa
     6721 ggtccaatgc ggtcaggtac ttgccttccg cgaaggcaat atatgacggg ctttccgcag
     6781 cgcgcgccgg gccacttgcg aggatgcaag cgcttgcgat caagactccg gcaaatagtc
     6841 gtctcacgcg gtgctcctga catgggcaat ggcgcggccg atgtccgcaa caatggtggc
     6901 ggcgttgtcg gcctgccaga ttgtccggga cggtgcgacg aagtcggcgc cttgttgcgc
     6961 aaatcgttcc gcctcggccg gcgtgtccac attccacgcc acgcaaggca cgacgaagac
     7021 ctcggaccac caggcgacga ggccctcgct ctccggcccg aaggcgacat aatcggcgcc
     7081 ggcttcggca agctccatgg cggcgtggcg gtccgccccg caatccacgc caatattggc
     7141 actttgcccg agtaggttgc gcgcggttgt atagcgttcc agatcggccg cgatatgcac
     7201 gccgtcggcg cccaggcgtt cggcgcgttc gaagtcattc tcaatgaggc aggcgatatt
     7261 ggccccctga atccggtcga tcagccgatc actcggctcg tcggtgggaa cttcttcggt
     7321 attttgactg ttatcgcaga gcacaacgca agccaccgat gtcgttgtca aagcttgggc
     7381 gagttgagat tcgagcttcg ccgtaatcgg cgcaggaact tgaaaataga ggcggcattg
     7441 agacttggcg tcagccatga ttagggggat cccacgggcg tgtttgcgat tggccggttg
     7501 ttttcaggga tttgtctcaa atcacacgct tcgcggtact gtcaaatcct gtgtgggccg
     7561 ccgatattgg ggttgtcgta cgctcgttga aggttgaaca tggggcaatc acatttcgcc
     7621 aggctgctgg gtcacacggc gagtcggtca taaaccggcg gcgcctagtg gggaggggcg
     7681 gacgcaatga agcaaccgga tgctctgcat cggctcgata cggcagttga ccaacttgag
     7741 ggtcatctga agcaggcctt tgcaggcggt ggagacgccg ccctcaagga cgaactgcga
     7801 tttctgaccg aggagcgcga tcagctcctt gccgatcttg aagcggagcg tagccgcgtt
     7861 cggcgcctca aggccgccaa tgatgaagtc tccggccgcc ttgaggttgt gatggggaca
     7921 ctgaaggata tgataccggc catgccgggc taggtttgcc gggcgaggga ggttcatggg
     7981 gcagatcagt gtcacgctga acggtcgtaa ctatcgactg gaatgcagcg aaggtgaaga
     8041 ggctcatctt ctcgagcttg ccgagtacct cggttcgcat gtcgacgaga tgcgtcggaa
     8101 gttcggccaa gtcggcgacg atcgcttgat cctcatggca tcgctggtgg tgaccgacga
     8161 gctctgggaa ttgcgccgcg aaatcgaaaa catgaagacc gcgttggccg aagcacgccg
     8221 ggacaagtcg gcatccgacg agacggcgaa gcatatgcag gcggacctgt ccgcccggat
     8281 cgacgcggca gccgaacggc tcgaggggct caatgccgag ctttccaaag gcgggcgcgc
     8341 gtcggacggc cgctagcggt tctgaaagat ttcttccata gtcagctctt ggcgggctgc
     8401 gtggtgcgtt aggagcattt attccagggc cctataagat tctacgggag ctgaccctga
     8461 cgaggaccgt ggtctttgtt actcggcgcc cacctacttt aagtagggac accggaatct
     8521 tcgctccgac ggccaacgcg gctctgccaa actttgcacg cacggtgcaa ttctgtattg
     8581 accccccgat gatccatctt cagcaagccg atttcccatg cgcattttgt tcctaggcga
     8641 cgttgtcggc cggtccgggc gcgcggcggt gctggagaca ttgccgaaat tgcgtgagcg
     8701 ctaccgcgcc gacttcgtcg tcgtgaacgg cgagaactcg gccggcgggt tcggcatctc
     8761 cgaagcgatc tttaatgagt tgctcgatgc gggcgccgac gtggtgacca cgggcaacca
     8821 tgtttgggat cagcgcgaag cgctcgtctt cattgagcgc catgaccgtc tcttacggcc
     8881 cgtcaacttt ccggccggca cgccgggtcg gggcgctggc ttgttcaagg ccgcgaacgg
     8941 catggacgtg ctggtggtca acgcgcaagg gcgcgtgttc atggccgatc tcgacgatcc
     9001 gttccgcgcg gtggagcagg aactcagcgc ttgcggtctg aaatccggca cggacgcgat
     9061 cctgatcgat ttccacgccg aggccacgtc ggaaaagcaa gccatggcgc atttcgtcga
     9121 cgggcgcgcg accgccgtca tcggcacgca tacgcatgta ccgaccgcag acgagttcgt
     9181 gctgaacggc ggtaccgcct atatcagcga cgccggcatg tgcggtgatt tcgattcgat
     9241 cctcggtatg gacaaagagg agccgattgc ccggttcctc accaagatcc actcgcggcg
     9301 gttcgcgcca tcgatgggcg aggcaacggt gtgtggtgtg gccattgagg tggacgacgc
     9361 caccgggctg gcaaaggcca tcgcgccggt gcggcttggc ggccggctgt cgcaggtcga
     9421 gcctgcgttc tggctcgacc cggcttaggc cgagcgccga cttggtgtgg cagtggcgtg
     9481 aggctcacgg cccttcgcca gaaggcgctg gaggcatcca atggcatcgt ccagatgcgc
     9541 cagcgcacga tccgtatcgt agcccttgtc gtcgagcgac agccgcgccc gcgcgatgtg
     9601 atcggcggcc tcagaacaca ggtcggggag gctcaaggtc tgctcggccg cctcgccggt
     9661 ggcgggtctg tgcatcggtg tcatggcctt gtcccctaac ctcgtggaat gcgccgactt
     9721 tcggcattcc caagccttta gttgtcggcc cattagggtt aaggcttcgc taagcggccc
     9781 ggcccgccac gtcgtcagtg cggggcgttc cgggccttga ccggtatggg cgcgagcgac
     9841 tagggatcag gccatggcag gccattcaaa attcaagaac atccagttcc gcaagggcgc
     9901 ccaggacaag aagcgcgcca agctgttctc caagctcgcc cgggagatca ccacggcggc
     9961 gaagctggga atgcccgacc cggccatgaa cccgcggctg cggacggcga tccaagcggc
    10021 gcgcgtccag aacatgccgc gtgacaatat cgaccgggcc atcaagaaga gccaggaggc
    10081 cggcggcgac aattacgaag aagtccgcta cgagggcttc ggcgtcagcg gcgtcggcgt
    10141 catcgttgag gcgcttaccg acaatcgcaa tcgcaccgcg agcgaagtgc gctccatctt
    10201 ctccaagcat ggcgggaacc tgggtgaaac cggcgcggtg tcgtttaatt tcgaccggct
    10261 tggcgccatc gagttcgagg ccggcaaggg aagcgccgac gacatgctgg aagcggcgat
    10321 cgaagcgggc gccgacgacg tagaaacgac cgacgagggc cacgtggtct actgcaatcc
    10381 ggatgagctg catcaagtcg gcaaggcgtt ggaagaacgc ttcggcgaga cgcgcgccat
    10441 gcagattctg tggcggccga agatcgaggt gccgatcgac gaggaagcgg gcgagaagct
    10501 cctacgcatg atcgacgctc tggaagagtc cgacgacgtg cagcaggtct acgccaattt
    10561 cgacgtctcc gacgacgtcc tgcaaaagct gactgcggcg tgaggctaaa gggctccccg
    10621 atgacccagc aggagcctgc cgaatacaaa catcttcgtg agcaatcggc gctgccgggc
    10681 aaaggcgggc ttgcgcgcat cggacgcgcc ttcgccaata cgctcggcgg atttcgcgag
    10741 ggactcgcca cggaagcagc gatcaagcag gaagcggcgc tgacgctgtt cgcgctgccc
    10801 gtctccttct tcgtagccga cagcgcgtgg atgtggattg ccctgatggg cagtctggtg
    10861 ctgctacttg ccgcggagct gctcaacacg gccatcgaac ggctgtgcaa tcacctgcat
    10921 ccggagaagc acgaggccat caaggtgacc aaggacctcg cctcggcagg cgtgttctgc
    10981 gtgatcgtgc tcaccgccat gatttggatc gttgcggtcg tcgtgcgggt ttgggcgggc
    11041 tagcgcagct tgcgcttgcg ggcttcgaaa gacaggccga gccacgcgcc tgaggccatg
    11101 ccgcttatca gcaacaaggt cgtcagcggc gcgccgatcg aggccgagag cggacgcgac
    11161 agaatccaga aatggatgag cgcggcgaca agcacgaaca tgcatgcggc ggccgcgccg
    11221 ccgagcgcgt aggcgatttt cgccttccac gcgccacgat cttcccagcg tctcgttttt
    11281 gccgccacgc ccaacacggt ggcgacaatg ttctcggtat cgtcgtcgaa gctttcatgg
    11341 cgcagcggca ctgcgttcag cgtggcgatc gctttgatat cctcgggcag ttgttcaggt
    11401 cttggcatgc gcgctccgcc gaccagaacc ggcaagacga gaagagagcc gccaagcgct
    11461 gcacggagct cggcacgcac gtgatcgtcc acgaaatcga ctgccgcaga cgacaaggcg
    11521 gacggaagca gcaacgctaa accgacccag atcaatggaa ggagcaaggc gccgagcagg
    11581 agcctcagcc aaatcggcgc cgctttcgcc aaaaactcac acctgccatg gccgtcgccg
    11641 tttttcgcta ccaatgacgg gccagttata acacgcttgg gccgcacgct ttagaccaag
    11701 cgtgtcttga tcggagcaac acatggtgga agacggcgca ggcacttggt cgcttcggga
    11761 ctctgagcgt gtcatcgccg ggaacgcggc gttgctgggc cggacccatc agctcgtcgc
    11821 catgacgcgt gtccgcaacg aggcgctgat cctgcccgac acgctcgact atctctcgag
    11881 ccacgtggat gcgatcatcg cgtacgacga tgccagcacc gacgagacgg tcgacatgtt
    11941 gcgcgcacat cccaaggtcg cgctgatcgt agccaatcag gcgtgggagc gcgatgtgga
    12001 tgcgcgtaaa cgtgccgagg ggcggcaccg cgggttgctg ctcgagatgg cgcgggcgca
    12061 agtgccgcat gactggatgt tctgtttcga cccggacgag cgcgtgaccg gtgatgtgcg
    12121 caggcatttg tacgggctcg acgccgattg tgatgcgtta cgggttcggc tgttcgacgc
    12181 ctatctgacg cccgaggatc aggcacccta cacatccgac caggaactgc ttggctttcg
    12241 caaatactat ggccccgagc agcgcgacat tctcatgttc tggcgcaatc gtgagggcat
    12301 cggcttcgcg caaggcgatg ggcgcacacc ccgcggcatg accgccgtca agacggacct
    12361 ctactgccag cattacggaa agtcgctgtc ggtcgcgcat tgggaggaga cgtgcgacta
    12421 ctatgtgcag cacttccctt acgagaccta tggcgcgaaa tgggaagcgc ggaagggaaa
    12481 ggcagttcac atcgagtcgg atttcggcaa cccgctccat gaatggggtg aggcgttgtt
    12541 cgcgcaggcc gtggcgatgc cgtcccagaa ataagacgcg aaatagaaaa aggcggaagc
    12601 cccgaagggc tcccgccttt agtgtgccca gccgtcgggg ggagagggcg gctaggccgg
    12661 aggagagtcg cgtttagctg tcgtcgtcat catcgtcttt gtctggcgtg ccgccgggac
    12721 cggtatccga cccgccgtca ggtgcggcct tcggcgggcc gtgcgggcct ttgccgtgcc
    12781 ggcccttgcc gcggtgacct ttgccccagc cgtgatggcg ggccatcttg gcgcggaacc
    12841 gcttctcgat ctccgtcacc ttctgaagct gctcaggggt gagcgtggtg cgcaattcgg
    12901 cgatcgcctt ctttagggct tcggcgctct cgccgcgctc aatggcgtta ttggcaaaac
    12961 gctcggccag ggaaaacggc acggcgttat cgccagaggc catcatgccg gtgccgccat
    13021 ggcgcatggg gcgcttcatg gtggcttgca gcgcgtcggt gaagtcgcgc caggcatcga
    13081 gctgattggc gcgaatgccg atctcggttt ccatgacgga aagcttcttg gccaagtggt
    13141 cggggccctt atggcgccaa ccatgcctac cgtgtttgcc gcccccgccg cgatggcaca
    13201 tcgacttgtc gccgtgtttg cctgcccagg cactttggcc ccagcgcccg tcatggccgc
    13261 cggccatggt gaccgccgca aggccggcga cgctcagaac ggccacgatg gcgatcgagg
    13321 caattcgctt ggagatgttc atttgcagca tcctcaatgg ttgatgtgcc ccttaggtat
    13381 cgcgagaaca ttgcctttgt agggctccgc gtatctgagt tttgctggcg gcaggagatt
//
LOCUS       DBNHVU010000095        14322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_781009, whole genome shotgun sequence.
ACCESSION   DBNHVU010000095 DBNHVU010000000
VERSION     DBNHVU010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 14322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14322
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_781009"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="AC16Y7_03640"
     CDS             <1..247
                     /locus_tag="AC16Y7_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5708506.1"
                     /translation="SFVAAKEIYSRITVPVTLVYGDDDWSRAEDRAETAKRIPGNGRI
                     MLNETGHFSALESPEEIARIVLKTAGSNDNAPARRSA"
     gene            268..786
                     /locus_tag="AC16Y7_03645"
     CDS             268..786
                     /locus_tag="AC16Y7_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708507.1"
                     /translation="MEITANHVHFFISYAVAFLVFTLIGKWYVWPSIKDRSAKLALTP
                     LLLYACLRINGLMFLMPGLVSPDLPSAFAVPTAYGDLTAVVLALLALACVRGESALAL
                     PMLWLFNTVGLLDLIYANIATFKNHVDPAQLGASYYLAVLNVPAMVVVHVMIFAYLLR
                     WRGTATAHGSAA"
     gene            831..1199
                     /locus_tag="AC16Y7_03650"
     CDS             831..1199
                     /locus_tag="AC16Y7_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708508.1"
                     /translation="MNRTHISLYYLCTYLLLGGFVLLFFPAEGLKLFFATGDYGTVFP
                     RVAGMLLAGLGMVIVAIIYTRSEALYPTTLVVRTFFLACLAAFYWMTNDPLFLVLLGI
                     VGFGFVLTLTSYLLDRKHQA"
     gene            complement(1203..2099)
                     /locus_tag="AC16Y7_03655"
     CDS             complement(1203..2099)
                     /locus_tag="AC16Y7_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5708509.1"
                     /translation="MRTLAIDTCIALATLLVFCVGYILWDSDANYYQVDVSTLGPGPQ
                     NDLIRYGHALVVDTPRYIGSGAQGPAMAYAGNDLACKSCHLKAGLQPFAAPFVSTAAT
                     FPMLVNNKVLTLPERINSCVELGLNGRPLPDVSREMAALIAYIEFVGKDTPEGVRLPG
                     SGLMPVKLPTEPPDSRRGIGVFAQHCTACHGENGQGQPKVSPEVGYYVPPLWGEGSFN
                     AAAGMGQIAYAASFIRANMPVGTDYQSPALTVQEAWDVAAYVISNPHPVAAPEPPPEP
                     DPEAELDLEVEADAATGQAASP"
     gene            2207..4528
                     /locus_tag="AC16Y7_03660"
     CDS             2207..4528
                     /locus_tag="AC16Y7_03660"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR5708510.1"
                     /translation="MTEAASGKAPLAVDPVCGMTVDTHVGKPTHDYQGHTYHFCSSGC
                     QAKFSASPERYLNKQDEPEPAPPGTLYTCPMHPEIVQEGPGPCPICGMALEPMGALPE
                     DVPNEELIDFTRRLWVALPLSLIIFAVDMSAHVFGIRLLPFISPKAEQWLMLALATPA
                     VLWCGWPFFVRGFESLRSGWLNMFTLIAMGTGAAYLYSLIATIAPGLFPESMKDAHGL
                     VPVYFEAAAVIVALVLEGQVLELRAREKTGGAIRALLDLAPKTALQVLKGGKTETIPL
                     AEVKVDDVLRVRPGDKIPIDGTVIEGRSAVDESMLTGEPIPVEKADGDAVTGGTLNGS
                     GSFDMRVDRTGAETTLSQVVNMVADAQRSRAPIQALADVVAGYFVPTVIAIAVIAFIV
                     WVFFGPAPQLAHALVAAVSVLIIACPCALGLATPISIMVATGRGAQAGVLVRNAAALE
                     RLAAVDTLVTDKTGTVTEGKPTLTGVDAAPGFDENEVLRLAAALEAGSEHPLAEAILR
                     GAEAKGLKLKKTQDFESITGQGVKGRVDGREVLLGNARLMEGAGIDVQALAQKADALR
                     RGGETVMFLGASGKLAGIVAVADPIKATAAEAIGKLHELGLRIVMATGDNETTAKAVA
                     AKLGIDEVHAGMRPEEKLSLIENLQKKGAVVAMAGDGINDAPALAKADVGIAMGTGAD
                     VAMESAGLTLLKGDLRGVVRGIKLARATMRNIKQNLFFAFFYNAIGVPIAAGVLYPIA
                     GIVLSPIIAAAAMSLSSVSVVGNALRLRQVKLD"
     gene            4603..7254
                     /gene="pepN"
                     /locus_tag="AC16Y7_03665"
     CDS             4603..7254
                     /gene="pepN"
                     /locus_tag="AC16Y7_03665"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HIR5708511.1"
                     /translation="MNDASRQTKHLKDYAPPDYLIDAVTLDVRLAPKAARIVSTLKMR
                     PNPEAATDGPPLVLDGEALTLERIVLDGEELAAGDYTLSAGKLTIPHVPAEPFTLEIT
                     TLCDPEANTELSGLYRSQGTYCTQCEPEGFRRITYFIDRPDILAVYTVRIEAGPDTPV
                     LLSNGNPVKKGGVPGTDRHYAVWHDPFPKPSYLFALVGGDLASIHDTFKTASGREVAL
                     AIYVEHGKEDRCAWAMESLKRSMRWDEERFGLEYDLDVFNIVAVSDFNMGAMENKGLN
                     IFNDKLVLARPDTASDADYASIESVIAHEYFHNWTGDRVTCRDWFQLCLKEGLTVFRD
                     QEFTEDVRMGPVMRIITARLLKSHQFPEDAGPLAHPVRPGSYIEINNFYTSTVYEKGA
                     EVCRMLQTLLGRDTFRKGLELYFERHDGEAATVEDFVGAMADASGRDLSQFMLWYTQS
                     GTPELACSLDYDAHAKTARLNVSQVVPPTPGQTSKEPMHIPLKVGLIGANGDELPLKL
                     NGDTLSDGLLEVTEREQSFEFHDVPAPPTPSLLRDFSAPVRLTTSLDADQIEFLMIHD
                     SDPFNRWQAAQTYAINLMTAAARNGHDTAPVTGTEAGRLAQSLATTVRDETLAPAYRA
                     EGLKLPAESDVARELARNVDTDAVHDARNALRATIGETIGDTLAEIYDTTAPRGSFSP
                     DPKSAGSRSLRNATLDLLVATQRPEEIDRAMRHYREASNMTDAIMALSILSHLDVPAR
                     DEALDDFYGRWQEEPLVLDKWFAVQARAARPDCVDTVRALLNHPKFSLKNPNRVRALI
                     GSFAQANPSGFNRADGEGYRLLASQALEIDRFNPHVAARLLGAFESWRTLEPGRQAHA
                     KAALESLSDQKLSIDSFEIVSKTLEGA"
     gene            7406..9694
                     /locus_tag="AC16Y7_03670"
     CDS             7406..9694
                     /locus_tag="AC16Y7_03670"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR5708512.1"
                     /translation="MARARSARGDGVFSIAGPQWQDLRALLDRHLPSAEVLRQLVPLL
                     LVGFGLVAAFGFAYQIGSSKDTALKSAERELALTADVSARNIMPATLSSSADWQGSLA
                     ASLPKGATRNGRTALLADAEGDIQARAPIGTSYRGNLLTVLGPQQPLTIMGANAGVLR
                     LTLLDGTDAIVTVRNVPQTDAQLAFIQPVSGALADWRGDAVIEISLLVCAGLVLLLIA
                     GGFLYLVPSSAAAEEDDQLAEELTEALPGCGVWRWNLARGHVHWSAPMYRLLGVEPLR
                     DAMPFGELSKALHPDDDLRRELDRHLREGIDLFDQCFRLRHADGHWVAVRLRGHITRQ
                     TNAEPYLTGMAVLADQDALPGAADSNARLRDAVETISEAFVLWDNQNQLVMCNSKYQQ
                     FHGLADDMVQPGSPYDDVIAAASEPIVSKRIAVAGKDADESRTYEAQLEDGRWLHINE
                     RRTRDGGYVSVGTDITVLKESQTRLAESEQQLRASVAELRLSRRELEHQKQQLVDLAE
                     KYALEKNRAEAANRAKSEFLANISHELRTPLNAVIGFSEVMQQALFGPLGHAKYQEYA
                     RDIYESGSYLLEVINDILDMSKIEAGRMTLDAGDVDVADIVEDSMKVVSQAAAEREIV
                     VERHGPAHLNIQADRRVLKQVFLNLLSNAVKFTRDSGRIDVNLSRGRGQVKIVIKDTG
                     IGIAEADLAKLGKPFAQVANQFSKSHQGSGLGLAISRALVELHGGTLKIKSREGEGTT
                     VTCCLPSRAAKPAPEEPLAASA"
     gene            complement(9712..12618)
                     /locus_tag="AC16Y7_03675"
     CDS             complement(9712..12618)
                     /locus_tag="AC16Y7_03675"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HIR5708513.1"
                     /translation="MTAFADRITECPEIYDAARGTDVLDGVLTSLRETPGLEAAADIA
                     ASPKLKALLEAVFSAAPYLAALSTRAPELLADCLTRDCDTHLSEAGAALSVDVASAPG
                     AKEVMALLRRYKQRSALLTALADLGGVWSTETTLKALSEAADVTVHAAVAYLFRKAHE
                     AGEISSPDPRGYFIIAMGKLGAMELNYSSDIDFMVFYDAERAGLTDGAEPSRFFVKLT
                     RELVRLVQEGTADGYAYRTDLRLRPDPGATQIALSTEAGLTYYESFGQNWERAALIKA
                     RIMAGDAVLGEDFLDQLSPFIWRKYLDFAAVSDIHAMKRRVHAHKGHGAIAVAGHDIK
                     LGRGGIRDIEFFAQTQQLIAGGRHPELRTRGTLETLAGLAEGQWIEKQAAEDLTAAYL
                     LFRRIENRLQMVGDQQTHIIPSDPAELDRVARLSGFDVTEDFGDALLTQFKLVEAHYG
                     ALFEKIPEPATSAPGIVVADDTDPGALASLEELGFTNPAAAVSAIQAWQSGRYAATRS
                     AKSRERLTKFLPHLLEAFSRTAEPDLALATFDKVIARLPAGLQLFSLFAANPSLLRLV
                     ADIMGTAPRLARIIGRRPRLLDVVLDPGFFGAVPTEPELKDIVSGAIGQAADYQDALD
                     LARVVGREQGFLIGVRVMSGTISARQAGAAYAALAETLIEVLSDRVADELVLQHGHMP
                     GGETAVIAMGKLGGNEMSAASDLDLITVYDFDDGDKQSDGARSLPGTQYYTRYTQRLI
                     AALSAPTAEGSLYEVDMRLRPSGSQGPVATKLSGFIDYQEKSAWTWEHLALTRARVVT
                     GPAALRKAIDDTIGQVLTRPRDRVAVAAEVQTMRGKIAQEKGTADIWSLKQVRGGLID
                     LEFIAQFLQLINAAERPDVLDQNTETALTKLSDAGVLSPDDAEILIPAARLYFTLTQV
                     LRLCLDKPFVAADAPLALRELLARATETPDFDALEVRLKDTLAAVHAAFDRIIA"
     gene            complement(12615..14072)
                     /locus_tag="AC16Y7_03680"
     CDS             complement(12615..14072)
                     /locus_tag="AC16Y7_03680"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5708514.1"
                     /translation="MTALTKLFRTTTFRLSLAYLALFSAAAVVAIVYLYWNTTVLLTR
                     QLNQTIDAELTGLAEQYRAGGLDQLVRIVAERSRTPGNSLYLVADGDGKQLAGNLSAV
                     SPQLWDSLGPVEFFYTRPAPGGVERRLAFANVFRLPGKHRLIVGRDIEDRRELARLIR
                     TTMLWGLGVMALVGIGGGYWVSRRLLARIDNLSATTRTIMEGDLSGRLPISGSGDELD
                     RLAQNLNLMLGRIEQLMAGLREVSDNIAHDLKTPLNRLRNRVEEALREPYEEATYREA
                     LERTIEEADDLIKTFNALLSIARLEAGAAGDNRDKLDLAVLVSDVAELYEPVAEERGI
                     ALEAEVEAPIVIRGDRQLLGQAIANLIDNALKYGAPSAQGGNGYAPEVGVQAQMDGDT
                     VEITVSDRGPGVPAAERERVLGRFVRLEESRSEPGSGLGLSLVAAVARLHGGVLRLED
                     NDPGLRVVLVLPLEGDALVNGAVTEPSTGAEPRPT"
     gene            complement(14056..>14322)
                     /locus_tag="AC16Y7_03685"
     CDS             complement(14056..>14322)
                     /locus_tag="AC16Y7_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008549675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5708515.1"
                     /translation="SHKVTRAGEAIILQPREFRLLEYLMKHAGQVVTRTMLLENVWDY
                     HFDPQTNVIDVHISRLRSKIDKNFSEPLLHTVRGAGYTIRDSAH"
BASE COUNT         2679 a         4713 c         4363 g         2567 t
ORIGIN      
        1 gagcttcgtc gcagcaaagg aaatctattc gcgcatcacc gtgcccgtca cgctcgtcta
       61 tggcgacgac gactggtcgc gcgcggaaga ccgcgccgag acggcgaagc gcatccccgg
      121 caatggccgg atcatgctca atgaaaccgg tcatttctcc gcgctggaga gtcccgagga
      181 gatcgcgcgg attgttttga agaccgccgg ctcgaacgac aacgcacccg cacggcggag
      241 cgcctaaacc gaccgaagga gatcagcatg gagatcaccg cgaaccatgt tcatttcttc
      301 attagctacg ccgtcgcatt ccttgtcttc acgctcatcg gcaagtggta cgtctggccg
      361 agcatcaaag accgcagcgc caagctcgct ctcacgccgc tgctcctcta cgcctgttta
      421 cgcatcaacg gattgatgtt tttgatgcca ggtctggtat cgccggatct gccgagcgct
      481 ttcgccgtac cgacggctta tggcgatctt accgcggtcg ttctcgcgct tcttgcactg
      541 gcttgcgttc gcggtgagag cgctctcgcg ctgccgatgc tttggctgtt taacacggtg
      601 gggctgctcg atctgatcta cgcgaacatc gcgacattca agaaccatgt cgatccggcc
      661 cagctcggag cttcatacta tctcgctgtc ctcaacgttc cggccatggt ggtcgttcat
      721 gtgatgattt ttgcctacct actcaggtgg agaggcactg caacggcgca cggctcggcg
      781 gcatagagac gactcgtcgt gcatcataag aaggcaaggg agacggtcag atgaaccgga
      841 cacacataag tctctactac ctatgcacct accttctcct aggcggtttc gttcttttat
      901 tcttcccggc ggaaggcctg aagctgttct tcgcgaccgg agactacggg actgtatttc
      961 cgcgagttgc cggcatgctc ctggcggggc tcggcatggt catcgttgca atcatctata
     1021 cgcgctctga ggcgctgtat ccgactacgc tcgtcgtgcg cacgttcttc ctggcctgcc
     1081 tcgccgcgtt ctattggatg acgaacgacc cgctgtttct cgtgctgctt ggtattgtcg
     1141 ggttcggctt cgtgctcacc ctgacgtcat accttctgga ccgcaaacac caagcatgac
     1201 gctcaggggc tagccgcttg accagtcgcc gcgtccgcct cgacctcaag gtcgagttcg
     1261 gcttccgggt caggctcagg cggaggttca ggcgctgcga cggggtgcgg gtttgagatc
     1321 acataggccg caacatccca cgcctcctgc accgtcagcg cgggcgactg gtagtccgtg
     1381 cccacgggca tgttggcgcg gatgaacgat gccgcgtagg cgatctgccc cataccggcg
     1441 gccgcgttga agctgccctc gccccacagc ggcggaacgt aatatccgac ctccgggctg
     1501 actttcggtt gcccctgacc gttctcgccg tgacacgccg tgcaatgctg agcaaagaca
     1561 ccgatgccgc ggcgcgaatc cggcggctcg gtcggcagct ttaccggcat cagcccgcta
     1621 cccggcaagc gcacaccttc gggcgtatcc ttgccgacga attcgatata ggcgatgagc
     1681 gccgccatct cgcggctgac gtcgggcaat gggcgcccat tcaacccaag ttcgacgcag
     1741 gaattgatgc gctccggcag ggtcagcact ttgttgttga ccagcatcgg aaaggtcgct
     1801 gccgtggaga cgaagggggc ggcgaagggt tgcaggcccg ccttgagatg acagctcttg
     1861 caggcgagat cgttccccgc ataggccatg gccggaccct gcgcaccgct gccgatgtag
     1921 cggggcgtgt ctacaacgag agcgtgtcca tagcggatga gatcgttctg cgggcccgga
     1981 ccgagcgtgg aaacatcgac ctggtagtag ttggcgtcac tgtcccaaag aatgtatccc
     2041 acgcaaaata cgaggagcgt cgcaagcgcg atgcaggtat cgatggcaag cgtccgcaac
     2101 tgaaaggcct cccaaaaacg gcatggtctt tagactatgc tacgtggcgc tgcgccatgc
     2161 ggcatgaatc actatatgaa ccttgagccc tcatggtctt gaaagcatga cagaagccgc
     2221 atccggaaaa gcccctctcg ccgtcgaccc cgtctgcgga atgaccgtcg acacgcatgt
     2281 ggggaagccc acacatgact accaggggca cacctatcac ttctgttcat ccggttgtca
     2341 ggcgaaattt tccgcaagtc ccgagcgcta tctgaacaag caggacgagc cggagcccgc
     2401 accgcctggc acgctctata cctgcccgat gcatccggag attgtgcagg aaggcccggg
     2461 cccctgcccg atctgcggca tggctctgga gccgatggga gccctgcctg aggacgtacc
     2521 gaacgaggag ctgatcgact tcacccggcg gctctgggtg gctctcccgc tatcgctgat
     2581 catcttcgcc gtcgacatga gcgcgcatgt cttcggcatc cggctcttgc ccttcatatc
     2641 gccaaaggcc gagcagtggc tgatgctggc gctggcaacc cccgcggtct tgtggtgtgg
     2701 ctggccattc tttgtgcgcg gctttgaatc gctgcgcagc ggctggctga acatgttcac
     2761 cctaatcgca atgggtacgg gcgccgccta tctctacagc ctcatcgcca caatcgcccc
     2821 cggcctcttc ccggagtcca tgaaagacgc gcatggcctg gtgcccgtct atttcgaggc
     2881 cgccgccgtt atcgtggcgc tggtgctcga gggacaggtg ctggagttgc gcgcccgcga
     2941 gaagacgggc ggtgccatcc gggcgctgct cgatcttgca cccaagacag ccttgcaggt
     3001 gctgaaaggc ggcaagaccg agaccatacc gcttgccgaa gtcaaggtcg atgacgtgct
     3061 gcgcgtgcgc cccggcgaca agatccctat cgacgggacg gtgatcgaag gccgcagcgc
     3121 ggtcgatgag tccatgctga cgggcgagcc cataccggtc gagaaagccg acggcgatgc
     3181 ggtgaccggc ggaacgctca acggctcggg ctctttcgac atgcgcgtgg accgcacggg
     3241 cgcggagacc acgctgtcgc aggtcgtcaa catggtcgcg gacgcgcagc gctcgcgtgc
     3301 accgattcaa gccttggccg atgtcgtcgc agggtacttc gtgccgactg tgatcgccat
     3361 tgccgtgatc gcctttatcg tctgggtctt ctttggtcca gcgccgcagc tcgcccatgc
     3421 tctggtcgcc gccgtcagcg tactcatcat cgcctgcccg tgcgcccttg gacttgccac
     3481 gccgatttcc atcatggtgg ccacgggccg cggtgcgcaa gctggcgttc tcgtgcgcaa
     3541 tgccgccgcg ctcgagcggc tcgccgcggt cgacacactc gtcacggaca agacggggac
     3601 cgtgaccgaa ggaaaaccaa ctttgaccgg ggtggatgct gcaccggggt tcgatgagaa
     3661 cgaagtgctg cgcctcgccg cggcgctcga agcaggcagc gagcatccgc tggccgaggc
     3721 aattctacgc ggcgccgaag caaagggctt gaaactgaag aagacgcaag atttcgaatc
     3781 catcacgggt caaggcgtca agggccgtgt ggacggtcgg gaggtcttgc tggggaatgc
     3841 gcgtctgatg gagggcgcgg ggatcgacgt tcaagcgctg gcgcaaaagg cggacgctct
     3901 acgacgaggc ggcgagacgg tgatgttctt gggtgcgagc ggcaagttgg ccggcatcgt
     3961 cgccgtggcc gatcctatca aggccaccgc cgcggaggcc atcggtaagc tgcacgagct
     4021 cgggcttcgc atcgttatgg cgaccggcga caatgagacg acggcaaagg ccgtcgcggc
     4081 caagcttggc atcgacgaag tgcacgccgg tatgcgaccc gaagagaagc tctctctgat
     4141 cgagaacttg cagaagaagg gcgctgtcgt cgccatggcc ggcgacggca tcaacgatgc
     4201 gccagccttg gccaaggcgg atgtcgggat cgccatgggt acgggcgcgg acgtcgccat
     4261 ggagagtgcg ggactgacct tgctcaaagg cgatctgcgc ggcgtggtcc gcggcatcaa
     4321 gctcgcccgc gccaccatgc gcaacatcaa gcagaacctg ttcttcgcct tcttctataa
     4381 cgccatcggc gtgccgatcg ctgcgggcgt gctctacccc atcgccggga tcgtgctctc
     4441 gcccatcatc gccgccgccg ccatgagcct gtcgtcggtg tcagtcgtcg gcaacgccct
     4501 gcgcctacgc caggtcaaac tcgactaatc gcaaatttgt gcttcgggct tggcattcgg
     4561 accatcgccc tctaagagcg acgcaaaaag gatcgcccgc ctatgaacga cgccagccgc
     4621 caaacgaagc acctgaagga ttacgcgccg ccggactatc tcatcgacgc cgtcacgctc
     4681 gatgtccgcc ttgcaccaaa ggccgcgcgc attgtttcca cgctaaaaat gcgccccaac
     4741 cctgaggccg cgacggacgg gccgccgctg gttctcgatg gtgaggccct gacccttgag
     4801 cggatcgtgc tggatggcga agagctcgcg gccggcgact acacgctatc ggccggcaag
     4861 ctcacgatcc cgcacgtgcc ggccgaaccc ttcaccctcg aaatcaccac gctatgcgac
     4921 ccggaggcca atacggaact ctccggtctt tatcgctctc aagggactta ttgcactcag
     4981 tgcgagcccg aaggctttcg ccggattacc tatttcatcg accggcccga cattcttgcc
     5041 gtctatacgg tgcggatcga agccggcccc gacacgccgg ttctcctgtc caatggcaat
     5101 cccgtcaaaa aggggggagt tccagggacc gatcgccact acgccgtctg gcacgacccc
     5161 ttccccaagc cgtcctatct gttcgcgctg gtgggcggcg accttgcctc gattcacgac
     5221 acgttcaaga ccgcctccgg gcgtgaggtg gcgctcgcca tctatgtcga gcacggcaag
     5281 gaagaccgct gcgcctgggc catggagtcg ttgaagcggt cgatgcgttg ggacgaggaa
     5341 cgcttcgggc tcgaatacga tctcgacgtg ttcaacatcg tcgccgtcag cgacttcaac
     5401 atgggcgcga tggagaacaa gggtctcaat atcttcaacg acaagctcgt gctcgcgcgc
     5461 cccgacaccg cgagcgacgc cgattacgcc tcgatcgaaa gcgtcatcgc gcacgagtat
     5521 ttccacaact ggacgggcga ccgcgtgacc tgccgcgact ggttccagct ctgcctcaag
     5581 gagggcttga ccgtattccg cgatcaagag ttcaccgagg acgtccgcat ggggccggtg
     5641 atgcgcatca tcacggcgcg gctcctaaag agccatcagt tcccggaaga cgccggaccc
     5701 ctggcccacc ctgtgcgccc tggctcgtat atcgagatca acaacttcta cacatcgacg
     5761 gtctacgaga agggcgccga ggtttgccgc atgctgcaaa cgctgcttgg ccgcgacacc
     5821 ttccgcaagg gccttgagct ctacttcgaa cgccatgacg gcgaagcggc caccgtcgaa
     5881 gactttgtcg gcgccatggc cgacgcctcc ggccgcgacc ttagccaatt catgctgtgg
     5941 tacacgcaaa gcggcacgcc ggagctcgcc tgctcgctcg actacgacgc acacgccaag
     6001 accgcacgac tgaacgtgag ccaagtcgtg ccgccgacgc cgggtcagac cagcaaggag
     6061 cccatgcaca ttccgctgaa ggtcggcctc atcggcgcga acggcgatga gctgccgctg
     6121 aagctgaatg gcgatacgct ttcggacggc ttgctcgaag tcaccgagcg ggagcagagc
     6181 ttcgagttcc acgatgtccc ggcgccgccg acgccttcgc tcctgcgcga tttttccgcg
     6241 cccgtgcgcc tcaccacgag cctcgatgcg gaccagatcg agttcctgat gatccatgac
     6301 agcgacccct tcaatcgctg gcaggccgca cagacctacg ccatcaatct catgaccgcc
     6361 gcggcgcgta acggccacga tacggcaccg gtgacgggca cggaggcagg ccgcctcgct
     6421 caatcattgg caaccacggt ccgcgacgag acgttggccc ccgcttaccg cgccgaaggg
     6481 ctgaagcttc cagctgagtc cgacgtcgcc cgcgaacttg cgcgcaatgt cgatacggac
     6541 gccgtccacg acgcacgtaa tgcactgcgc gccacgatcg gcgagaccat cggcgacacg
     6601 ctcgccgaga tctatgacac gaccgcaccc cgaggatcct tctcgccgga cccgaagagc
     6661 gccggctcgc gtagcttgcg gaatgccacg ctcgacttgc tggtggcgac gcaacggcct
     6721 gaagagatcg atcgcgccat gcgccactac cgtgaggcgt cgaacatgac ggacgccatc
     6781 atggcgctgt cgatcctgtc tcacctcgac gttccggcgc gcgacgaggc gctcgatgat
     6841 ttctacggcc gctggcagga agagccgctc gtcctcgaca aatggttcgc ggtgcaggcg
     6901 cgcgccgccc ggcccgattg cgtggacacc gtgcgtgcac tcctgaatca cccgaaattc
     6961 tcgctcaaga accccaaccg cgtgcgggcg ctgatcggct ccttcgcgca agccaacccg
     7021 tccggcttca accgcgccga tggcgaaggc tatcgattgc tggcctccca ggccctggag
     7081 atcgatcgct tcaaccccca tgtggcggcg cggctcttag gggctttcga gagctggcgg
     7141 acgctggaac cgggccgcca ggcccacgcc aaggccgctt tggagagcct ttccgaccaa
     7201 aaactctcca ttgacagctt cgagatcgtc tccaagaccc tcgaaggcgc ttgagagtcg
     7261 cacaggctgt caacctctta ttaaccgggt ttccacagat ttcctctaga cagatgcggt
     7321 cgttctcgat tccatggacg gaagcgattc ggagatgcgg caacttctcc gagactgcga
     7381 aggcaggaga agcaaggggg accgaatggc gcgggcgcgc tctgcccgag gcgacggagt
     7441 cttttctatc gcggggccac aatggcagga cctgcgggct ctactcgatc gccacctgcc
     7501 ctctgccgag gtcctgcggc agctcgtgcc cttgctcttg gtgggtttcg gcctcgtggc
     7561 cgctttcggc ttcgcctatc agatcggcag cagcaaggac acagccctca agtcggctga
     7621 gcgagagctc gccttgacgg ccgacgtttc ggcccggaac atcatgcccg cgacattgag
     7681 ttcgagcgcc gattggcagg gatcgctggc cgcgagcctg cccaaaggcg ccacgcgcaa
     7741 tgggcgcacg gcgcttctcg ccgacgcgga gggcgatatc caagcccgcg cccccatcgg
     7801 cacctcctac cggggcaacc ttctcactgt tcttggacca cagcagcctc tcaccatcat
     7861 gggcgccaac gcgggcgttc tacgcctcac tctgctggac gggaccgacg cgattgtcac
     7921 cgtccgcaac gtgccgcaga ccgacgcgca gctggcgttc atccagccgg tgagcggcgc
     7981 gctcgccgat tggcggggcg acgccgttat cgaaatcagc ctgctggtct gcgccggcct
     8041 cgtcttgctc ctgattgccg gcgggttcct ctatctggtg ccgtcgtccg cggccgccga
     8101 ggaggatgac cagctcgccg aagaactgac agaagccctg cccggctgcg gtgtctggcg
     8161 ctggaacctc gcccgtggac acgtgcactg gtccgcgccc atgtatcgct tgctcggagt
     8221 cgaaccgctt cgcgatgcca tgccgttcgg cgaactgtcc aaggccctgc atccggacga
     8281 tgatctgcgc cgcgagctcg accggcacct acgcgagggt atcgatcttt tcgaccaatg
     8341 cttccgccta cgccacgctg acggccattg ggtcgcggtc cgcctacgtg gtcacatcac
     8401 gcgtcagact aatgcagagc cttatctcac gggcatggcc gtgctcgccg atcaagatgc
     8461 acttcccggc gccgcggatt ccaatgcccg gctacgcgac gcagtcgaga ccatctcgga
     8521 agcctttgtc ctgtgggaca atcagaacca gctggtcatg tgcaacagca agtaccagca
     8581 gtttcacggc cttgccgacg acatggtgca gcctggcagt ccctatgacg acgtcatcgc
     8641 tgccgcgtcc gaacctatcg tctccaaacg tatcgccgtc gccggcaagg acgccgacga
     8701 gtcccggacc tatgaagcac aactcgaaga cggccgttgg ctacatatca atgagcggcg
     8761 cacgcgggat ggcggctatg tgagcgtcgg caccgacatc accgtcttga aggaaagcca
     8821 gacgcgcctt gccgagagcg agcagcagct tcgcgccagc gtcgccgagc tcaggctgtc
     8881 ccgccgcgag ctggagcatc agaagcagca actcgtcgac ttggccgaga aatatgcact
     8941 cgaaaagaac cgggccgagg ccgcgaaccg cgccaagtcc gaattcctgg caaatatcag
     9001 ccacgagtta cgcacgccgc ttaacgccgt catcggcttc tcggaggtca tgcagcaggc
     9061 gctgttcggc ccgctcggtc atgcgaaata tcaggaatac gcccgcgaca tttacgaaag
     9121 cggcagctac ctgcttgagg tcatcaacga cattctcgac atgtcaaaga tcgaggccgg
     9181 gcgcatgaca ctcgacgccg gcgatgtgga cgtcgccgac atcgtcgagg atagcatgaa
     9241 ggtcgtgagc caggccgccg ccgaacgcga gatcgtggtc gagcgtcatg gccctgccca
     9301 tctcaacatt caggccgacc gcagggtgct caagcaggtg ttcctcaatc tcctgtcgaa
     9361 cgcggtcaaa ttcacccgcg acagtgggcg catcgacgtc aatctttcac gcggccgcgg
     9421 tcaagtgaag atcgtcataa aggatacggg catcggtatc gccgaagccg accttgccaa
     9481 gctcggcaaa ccgttcgcac aggttgcgaa ccagttctcc aagagccatc agggcagcgg
     9541 cctcgggctg gccatctccc gcgcgctggt cgagctgcat ggcggcacac tcaagatcaa
     9601 gagccgcgaa ggcgaaggca cgacagtcac ctgctgcctg ccctcgcgag ccgcgaagcc
     9661 agcgcccgaa gagccgctcg ccgcgagcgc ttaaagccca ccacgccgtc cttacgcgat
     9721 aatgcgatcg aacgccgcgt gcacggccgc gagcgtgtcc ttcagcctga cctccagcgc
     9781 atcgaaatcc ggcgtctcgg tcgcgcgcgc cagaagctcg cgcagagcga gcggtgcgtc
     9841 ggctgccacg aagggcttgt ccaggcaaag ccgcaagacc tgtgtcagcg tgaaatacag
     9901 acgcgccgcc gggatcagga tctcggcgtc gtccggcgac aggacgcctg cgtccgaaag
     9961 cttggtcagg gccgtctccg tgttctgatc gagaacgtct ggacgctccg cagcgttaat
    10021 gagctgcagg aactgagcga tgaactccag atcgatcagc ccgccgcgca cctgtttgag
    10081 gctccagatg tcggccgttc ccttttcctg cgcgatcttg ccccgcatgg tctggacctc
    10141 ggcggccacc gcgacacggt cccgcggccg ggtcaggact tgtccgatgg tgtcgtcgat
    10201 cgccttgcgt aaggcggcag ggcccgtgac cacgcgcgcg cgcgtcaacg ccaggtgctc
    10261 ccaggtccac gcggacttct cctgatagtc gatgaagcct gacagctttg tggcgacggg
    10321 cccctgactt cccgaaggac gcagccgcat gtcaacttcg tagagcgatc cctccgctgt
    10381 cggggccgaa agtgctgcaa tcagacgctg cgtatagcgc gtgtagtact gcgtccccgg
    10441 cagacttctc gcgccgtcag actgcttgtc gccatcgtca aagtcgtaaa cggtaatgag
    10501 atcgaggtcg gatgcggccg acatctcgtt ccctccaagc ttgcccatgg caataaccgc
    10561 cgtctcgccg cccggcatgt gcccgtgttg gagcacaagc tcgtctgcca cacgatccga
    10621 aagaacctcg atcagcgttt cggccagcgc cgcataggcg gcgcccgcct gccgcgccga
    10681 gatggtgccg gacatcaccc gaacgccgat aaggaatccc tgctcacggc cgacaacgcg
    10741 cgcaaggtct agcgcgtctt gataatcggc ggcttgcccg atggcgccgc tgacgatgtc
    10801 cttgagttcc ggctcggtgg gcaccgcgcc aaagaaaccc ggatcgagga ccacatcgag
    10861 gagcctcgga cgcctcccga tgatcctggc gagacgcggc gccgtgccca tgatgtccgc
    10921 gacgagacgt aggagactcg gattggccgc gaacagcgag aacagttgaa gccccgccgg
    10981 caaacgcgca atgaccttat cgaaggtggc gagcgcgagg tccggctcgg cggtgcggct
    11041 gaacgcttcg agcagatgcg ggagaaactt ggtcagcctt tcgcgggact tggcgctgcg
    11101 cgttgcagca tagcgcccgg attgccaagc ctggatggca gacaccgccg cagccgggtt
    11161 ggtaaagccg agctcttcga ggctcgcaag cgcacctgga tcagtgtcgt cggcaacgac
    11221 gattcccggc gccgacgtcg cgggctcggg gatcttctcg aacagcgcgc cgtaatgcgc
    11281 ctcgaccagt ttgaactggg tcaaaagcgc atcgccaaag tcttctgtga catcgaaccc
    11341 agacagccgt gcaacgcgat cgagttctgc cggatcgctt ggaatgatgt gcgtctgctg
    11401 atcgccaacc atctgcagcc ggttctcgat acggcgaaat aacagatagg ccgccgtcag
    11461 gtcttcagcc gcttgtttct cgatccactg cccctctgcg aggcccgcca aggtttcaag
    11521 cgtgccgcgc gtacgaagct cggggtgacg gccgccggcg atcaactgct gggtctgggc
    11581 aaagaactcg atatcgcgaa tcccgccgcg gccaagttta atgtcgtgac cggcaacggc
    11641 gatggcacca tggcctttgt gggcatgcac gcgccgcttc atagcgtgga tgtcggagac
    11701 agccgcgaaa tccaaatatt tgcgccagat gaagggcgaa agctggtcga ggaagtcttc
    11761 gccgagcaca gcatcgcctg ccatgatgcg tgccttgatc agcgccgccc gctcccagtt
    11821 ctggccgaag ctctcgtaat aggtgagccc cgcctccgtc gagagcgcaa tctgcgtggc
    11881 gcccggatcg ggcctcagcc gcaagtcagt gcggtaggca tagccgtccg ccgtaccctc
    11941 ttggacgagc cgcacaagtt cgcgcgtcag cttaacgaag aaccgtgacg gctcggcgcc
    12001 gtcggtgagc ccggcgcgtt cagcgtcgta aaacaccata aagtcgatgt cgctcgaata
    12061 gttgagctcc atcgcgccga gcttgcccat ggcgatgatg aaatacccgc gcgggtccgg
    12121 cgaggagatt tcacccgcct catgggcttt gcggaacaga tatgcgacgg cggcatgcac
    12181 ggtcacgtcc gcggcctcgc tgagcgcctt caacgtcgtc tctgtcgacc acacgccccc
    12241 gagatcggcg agcgccgtca gcagcgcgct gcgctgcttg taccggcgca gcagtgccat
    12301 aacctccttg gcgccgggcg cgcttgccac gtcgacagaa agcgcggcgc ccgcctcact
    12361 cagatgcgtg tcgcaatcgc gcgtgaggca gtcggccagg agttccggcg cgcgcgtgga
    12421 aagggccgcg agataaggcg ccgcggagaa gacagcctcc agcaaggcct tgagctttgg
    12481 cgatgcagca atgtcggccg cggcctcgag ccccggcgtc tcgcgcaaag atgtaagaac
    12541 gccgtccaac acatcggtgc cgcgggccgc gtcataaatt tcagggcatt cggtaatgcg
    12601 gtcggcaaac gccgtcacgt gggcctcggc tcagctcccg tggacggctc agtgaccgcc
    12661 ccgttgacga gcgcgtctcc ctccaggggc agaacgagga cgacgcggag gccgggatcg
    12721 ttgtcctcaa gcctcaggac gccaccatgc aaccttgcca ccgccgcgac aaggctcaag
    12781 ccaagtccgc tcccaggttc cgaccggctt tcctcgagcc gcacaaaccg tccgaggaca
    12841 cgctcgcgtt cggccgccgg cacgccaggc ccgcgatcgc tcacggtaat ctccactgtg
    12901 tcaccgtcca tttgtgcctg cacgcccacc tccggcgcgt agccattgcc cccttgcgcg
    12961 gagggcgcgc catatttgag cgcattgtcg ataaggttcg cgatggcctg tccaagcagc
    13021 tgccggtcac cgcgaatgac gatcggcgcc tcgacctccg cctcgagggc gatgccccgt
    13081 tcctccgcca ccggctcgta aagctccgcg acgtcgctca ccagcactgc aagatcgagc
    13141 ttgtcgcgat tgtcgccggc ggcgcccgcc tcaagccggg caatggagag cagcgcgttg
    13201 aatgtcttga tgagatcgtc ggcctcttcg atggtgcgct ccaacgcctc cctgtaggtc
    13261 gcctcctcat aaggctcgcg caacgcctcc tcgacacggt tgcgcaaccg gttcagcggc
    13321 gtcttcaggt cgtgcgcgat attgtcggag acttcgcgca accccgccat caattgctcg
    13381 attcgtccca gcatgaggtt gaggttctgc gccagccgat cgagctcatc gcctgagccg
    13441 ctgatcggta gccgccccga caagtcgcct tccatgatcg tgcgcgtggt cgccgagagg
    13501 ttgtcaattc gcgcgagcag ccgtcggctc acccaataac caccgccgat accgaccagc
    13561 gccatgacac caagccccca caacatcgtc gtgcggatga ggcgcgccag ctcacgccgg
    13621 tcctcgatat cgcggccgac gatcaaccgg tgcttgccag gcaggcgaaa gacgttggcg
    13681 aaggccagcc gccgctccac gccgccgggc gccgggcgcg tgtagaagaa ctccacgggc
    13741 cccaaactgt cccagagctg cggcgacacc gcgctcaagt tgccggcaag ctgcttcccg
    13801 tctccatcgg cgacgagata gaggctgttg cccggcgtgc ggctccgctc ggccacgatc
    13861 cgcacgagct gatcaagccc gccagcgcga tactgctcgg cgagccccgt caactccgca
    13921 tcgatggtct gattgagctg ccgcgtgagc agcaccgtcg tgttccaata aaggtagacg
    13981 atcgccacca cggcggcggc gctgaacagc gcaagatacg ccagcgaaag acggaacgtc
    14041 gtcgtccgga agagcttagt gagcgctgtc acggatggtg tatccggcgc cgcggaccgt
    14101 gtgcaggagg ggttcgctga agttcttgtc gatctttgac cggagccgcg agatgtggac
    14161 gtcgatgaca ttggtttgcg gatcgaaatg atagtcccac acattctcca gcagcatcgt
    14221 gcgcgtgacc acctgcccgg cgtgcttcat caagtactcc agaaggcgga actcgcgcgg
    14281 ttgcaggata atcgcctcgc cggcccgcgt caccttatgg ga
//
LOCUS       DBNHVU010000096        10672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1301627, whole genome shotgun sequence.
ACCESSION   DBNHVU010000096 DBNHVU010000000
VERSION     DBNHVU010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 10672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10672
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1301627"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /gene="glmM"
                     /locus_tag="AC16Y7_03690"
     CDS             complement(<1..284)
                     /gene="glmM"
                     /locus_tag="AC16Y7_03690"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596548.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR5708516.1"
                     /translation="MARKFFGTDGIRGSANSNPMTSEVALKVGMAAGKLFTNGAYRHR
                     VVIGKDTRLSGYMIESAMVSGFTAVGMDVFQLGPMPTPAVAMLTRSLRADL"
     gene            complement(438..1505)
                     /gene="folP"
                     /locus_tag="AC16Y7_03695"
     CDS             complement(438..1505)
                     /gene="folP"
                     /locus_tag="AC16Y7_03695"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR5708517.1"
                     /translation="MGDQKLYLRPIGFLYGPPADAAVEDGLALPLAGGPIAFTEAVLI
                     EGDPARSQRRLITASALGEVADADLANLRVRVTDPRPPFAGLDLSRPVLMGIVNVTPD
                     SFSDGGLYDQTEGAAAHAAELASDGAAIVDIGGESTRPGADTVADDDELSRVVPVLKA
                     LKGSPAVISIDTRKSAVARAAARAGAKILNDVSALTYDPACLDVAVEEGLDLVLMHAQ
                     GEPKTMQDNPTYDDVALEVFDYLEERIAACEAAGVPRARIAADPGLGFGKTLAHNLAL
                     LANVSLFHGLGVPLLIGASRKRFIGGLGQEKDPKSREPGSHAAAIASAAQGAQAFRVH
                     DVASSRQALAVWRASMFGTEN"
     gene            complement(1583..3517)
                     /gene="ftsH"
                     /locus_tag="AC16Y7_03700"
     CDS             complement(1583..3517)
                     /gene="ftsH"
                     /locus_tag="AC16Y7_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014286937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR5708518.1"
                     /translation="MNSNFRNFVIWVFIGLLLVALFNLFNTQGAQVRGNEISFSQLLS
                     EVDAGNVQDVTIAGNQIAGHYTDGRNFQTYAPNDPNLVEKLNTKGVKITAKPSEEDVP
                     SLFGVLISWFPMLLLIAVWIFFMRQMQSGGGRAMGFGKSKAKLLTERHGRVTFDDVAG
                     VNEAKEDLQEIVEFLRDPQKFQKLGGKIPRGALLVGPPGTGKTLLARAIAGEANVPFF
                     TISGSDFVEMFVGVGASRVRDMFDQAKKNAPCIIFIDEIDAVGRHRGAGLGGGNDERE
                     QTLNQLLVEMDGFEANEGIILVAATNRPDVLDPALLRPGRFDRQVVVPRPDIIGREKI
                     LKVHVRKVPLAPDVDLHVIARGTPGFSGADLANLVNEAALLAARRSKRLVTQHEFEDA
                     KDKVMMGAERRSMVMTEEEKTLTAYHEGGHALVAIHQPASDPVHKATIIPRGRALGMV
                     MRLPERDMLSHTRAKLKADIAVAMGGRVAEEIVFGYEKVTSGASSDIKMATQLARAMV
                     TQFGMSDKLGPLAYGDNEEEVFLGHSIARQQNLSDETQSMVDEEIHRIVDEGFETARK
                     VISENIDDLHVVARGLLEYETLSGKEIKDLLNGKPPVRDFPAEEESPSGPTSAVPTAG
                     RGKKPPEPDTGAGGMEPQPT"
     gene            complement(3668..4993)
                     /gene="tilS"
                     /locus_tag="AC16Y7_03705"
     CDS             complement(3668..4993)
                     /gene="tilS"
                     /locus_tag="AC16Y7_03705"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR5708519.1"
                     /translation="MTAAPVTHDDAEVLFAPLAHLPRVALAVSGGPDSLALMHLTAQW
                     RAARSDGPELVVLTVDHGLRTDSRDEALLVSRAAEAASLLHVILTWEHDGVDEGLQVR
                     AREARYDLMAAYTHAHDIGALVTAHHLDDQAETFLMRLARGSGLDGLAAIPEEGSWAG
                     LTLLRPLLDVPKARLVATVEAAGIAYVTDPSNIDPKFERGRVRSAMETLAGFGLSADV
                     IALSAKRLRRARAALDAAACDFLDGHAETSAAGYASVDAGATDALPEEVALRVLSRLL
                     ATVGGGMEPVRLAKLEALYAALKESPDKAHTLARCRIAPKGGRLFVFREMRHEGLPEL
                     ELSPGERTLWDNRFAIELGAAEEAPIIVRALGEEGVRVLREREAMPASLPGPAAQALP
                     ACWRGRRLLCLPDFGQVEPFLTPLSRHEEGLDCRARFLTGLGATKGPGY"
     gene            complement(4990..6069)
                     /locus_tag="AC16Y7_03710"
     CDS             complement(4990..6069)
                     /locus_tag="AC16Y7_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024572.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5708520.1"
                     /translation="MPSRSHLWRAVLAAFALAVLLPMGASLAEETESSKTTDDVATRV
                     ARLEAEISALRIMVGALEAAVIARPSLAPDGVDATPAGDDDLKGRVRGLEVQIGALTR
                     QMDTFLSKLNEMSEAAPADKTMPKPKVAAPDPKPERAKEKVAKDETAKKDAPAMITDP
                     EPRPRAATAPKPNVPEPKVPETKIAEPKVADPKVPAPERKPPIVEAKRELKPEPEPQD
                     EDVPIPPSLAPVVPPPAKIADPSKPRWYGPPPQPSDDQATPSSTITTGSLPDKASPAQ
                     YWVGESQYLRKQYQKAADSFLDGYRKYSGSDKASDTLLRLGMSLAELGKTEAACATFK
                     KLGARFPNSAQRGQAKTAAGKAGCP"
     gene            complement(6171..6680)
                     /locus_tag="AC16Y7_03715"
     CDS             complement(6171..6680)
                     /locus_tag="AC16Y7_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR5708521.1"
                     /translation="MRFGGIGRACVVVAVIILAFSAGACSRQPQQPKGFGLGGAAPAT
                     PGSPEDFAANVGDVVYFQGDSVVLTGHSRAILRQQVRWLNQHPQYQVMIAGHASEPGS
                     RQHNLVLGAQRAMAVKTFLVRHGLRAGRLHTVSYGRERPVAACDDISCWSRNRRAQTV
                     LTGGAVASY"
     gene            complement(7450..7923)
                     /locus_tag="AC16Y7_03720"
     CDS             complement(7450..7923)
                     /locus_tag="AC16Y7_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HIR5708522.1"
                     /translation="MGGALQPGGALSNRRARRRSRVPMSEINVTPLVDVMLVLLIIFM
                     VTAPLLTVGVDIDAKASLPDVNLPRTEAGQVAPERAPLSITIEADGTLYLQNAQISFE
                     DLAPKLAAIRDAGGTAQVWVRGDTQSAYGDVARVLVRVKEAGLPVTLLTDQVAPR"
     gene            complement(7932..8633)
                     /gene="tolQ"
                     /locus_tag="AC16Y7_03725"
     CDS             complement(7932..8633)
                     /gene="tolQ"
                     /locus_tag="AC16Y7_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HIR5708523.1"
                     /translation="MNSDVAVDLSIWELFKNAHIVVQIVIVGLLAASVWSWAIIIEKF
                     FLFTKTRKEADKFEQVFWSGQSLDELYQALAQRRNTGMAALFVAAMREWKRSTERDSG
                     DRSTKPMPGMQHRIEKVMDVTVSREVERLERRLTFLATVGSTAPFIGLFGTVWGIMTS
                     FQAIATTKNTGLAVVAPGIAEALFATALGLLAAIPAVIFYNKFNAEVGRHAARLEGFA
                     DEFAAILSRQIDKTL"
     gene            complement(8848..9297)
                     /gene="ybgC"
                     /locus_tag="AC16Y7_03730"
     CDS             complement(8848..9297)
                     /gene="ybgC"
                     /locus_tag="AC16Y7_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HIR5708524.1"
                     /translation="MSSWPDLAGRIEGDTHILPVRVYFEDTDFAGLVYHANYLKFCER
                     GRSDFIRLLGIHHQDLANPDQGEASVFVVRRVEIDYLKPAKLDDVLEVVTACVETGAA
                     SLTLSQEVRRDGVVLVRAKVVVVLVSEAGKPQRLGQLVRGALERFVK"
     gene            complement(9294..10343)
                     /gene="ruvB"
                     /locus_tag="AC16Y7_03735"
     CDS             complement(9294..10343)
                     /gene="ruvB"
                     /locus_tag="AC16Y7_03735"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR5708525.1"
                     /translation="MSERVLAAAKKDDDQQEAALRPQTLAEFIGQEKVRSNLKVFIDA
                     AGSRREALDHVLFAGPPGLGKTTLAQIVARELGVNFRATSGPVIAKAGDLAALLTNLE
                     ERDVLFIDEIHRLSPAVEEILYPAMEDFQLDLIIGEGPAARSVRIDLAKFTLVAATTR
                     TGLLTTPLRERFGIPIRLEFYTVEELEEIVRRGARLLQLPLTPEGAHEIARRARGTPR
                     VAARLLRRVRDFAAVDGNGAVDAKLAGSALSQLEVDGRGLDALDHRYLKLIAVNYGGG
                     PVGIETIAAGLSEPRDAIEEIIEPYLLQQGFIARTPRGRVLTGLAFEHLGVTPPAQPS
                     QFPLFNGGENGGEAE"
     gene            complement(10340..>10672)
                     /locus_tag="AC16Y7_03740"
     CDS             complement(10340..>10672)
                     /locus_tag="AC16Y7_03740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction ATP-dependent DNA helicase
                     RuvA"
                     /protein_id="HIR5708526.1"
                     /translation="AADLANAVVLQDKAQVARAQGVGQKVAARIVAEMKDKMPALAPA
                     MAPGSGPAPIAILPEGLAARDAVSALTNLGYAHGEAAAAVSSAIGVAGNEAKAEELIR
                     IGLKELAG"
BASE COUNT         1952 a         3573 c         3326 g         1821 t
ORIGIN      
        1 agatcggcgc gcaaggagcg ggtcagcatc gccacggccg gcgttggcat cggcccgagc
       61 tggaatacat ccatgcccac ggcggtgaag cccgacacca tggcggattc gatcatgtag
      121 ccggacaagc gcgtgtcctt gccgatcacc acccgatgcc ggtaggcgcc attcgtgaac
      181 agcttgccgg cagccatgcc gaccttgagc gccacctccg aggtcatcgg attcgagttg
      241 gcgctgccac ggattccgtc cgtgccaaaa aacttacgag ccatcccggt ctttcccccg
      301 attacgcggc caggctgctc cttccctcgg acttgcccgg cgcggggagg gattatgctc
      361 ccggtcccca tcgagttgca ttcaaataat gtaagcaaaa attacacgcc tggatcgaga
      421 ttggcgtgga ttccgcctta attttcagta ccaaacatgc tcgcgcgcca gactgcaagg
      481 gcttggcggc tgctggccac atcgtggacg cggaaggctt gggccccttg cgccgcggac
      541 gcgatggccg ccgcgtgact gcccggttcc cgcgatttcg ggtccttctc ctggcccagc
      601 ccgccgataa agcgcttgcg cgaggccccg atcaggagag gcacgccaag cccgtgaaac
      661 aggctgacat tggcgaggag cgcgagattg tgggccagcg tcttgccgaa gccgagcccc
      721 ggatcggcgg cgatgcgggc gcggggcacg cccgccgcct cgcaagccgc aatgcgctcc
      781 tcaaggtagt cgaagacctc cagcgccacg tcgtcgtagg tcgggttgtc ctgcatcgtt
      841 ttgggctcgc cttgggcatg catcaggacg aggtccagcc cctcctccac cgccacgtcg
      901 agacaggccg gatcgtaggt cagcgccgag acgtcgttga ggatcttggc gcccgcgcgt
      961 gctgccgcgc gcgcaaccgc cgatttccgg gtatcgatgg agatcaccgc gggcgagccc
     1021 ttcaacgcct ttagcaccgg caccacccgg gacagctcgt cgtcgtctgc gaccgtatcg
     1081 gcgcccggcc gcgtcgattc cccgccgata tcgacgatcg ccgcaccgtc ggaggcgagc
     1141 tcggcggcat gggccgcagc gccctccgtc tgatcgtaga gcccaccgtc ggagaagctg
     1201 tccggcgtga cattgacgat gcccatgagc acgggccgcg aaaggtccag cccagcaaac
     1261 ggcggccggg ggtccgtcac ccggacgcgc agattggcaa gatcggcgtc ggccacctcg
     1321 ccgagcgcgg acgccgtgat gagacgccgt tgcgaccgcg ccggatcgcc ttcgatcaga
     1381 accgcttcgg tgaaggcgat cggcccgccg gcgagcggca gagcaagacc gtcctcgacc
     1441 gcagcgtccg cgggcggtcc gtacaagaac ccgatgggtc tgagatagag cttttgatcc
     1501 cccatggcgc ctgagcctta ccacaaagca acatgcccgg cgaaaggccg ggcatgcgca
     1561 aaagcaaatt ttcgaaagcc gcctaagtcg gctggggctc catgccgccc gcgccggtgt
     1621 ccggctccgg cggcttcttg ccccggcccg cggtcggcac ggcggaggtc ggcccagacg
     1681 gcgactcttc ctccgcgggg aagtcccgta ccggaggctt gccgttcaga aggtccttga
     1741 tctccttgcc ggagagcgtc tcgtattcga gaagcccgcg cgccaccacg tgcagatcgt
     1801 cgatattctc agagatgacc tttcgcgccg tctcgaagcc ttcgtccacg atgcgatgga
     1861 tttcctcatc gaccatggac tgggtctcgt cggacaggtt ctgctgccgc gcgatggagt
     1921 ggccgaggaa cacttcttcc tcgttgtcgc cataggcaag cggcccgagc ttatcggaca
     1981 tgccgaactg cgtgaccatg gcgcgcgcga gctgcgtggc catcttgatg tcggacgacg
     2041 cgcccgaggt caccttctcg tagccgaaca cgatctcctc ggcgacgcgg ccacccatgg
     2101 cgacggcgat atccgcctta agcttcgcgc gggtgtgaga cagcatgtcc cgctccggca
     2161 ggcgcatcac catgccgagc gcgcggccgc gcggaatgat cgtcgccttg tggacgggat
     2221 cggacgccgg ctgatgtatc gcgaccagcg catgcccgcc ttcatgatag gcggtcagcg
     2281 tcttctcttc ctcggtcatg accatggagc ggcgctccgc acccatcatg accttgtcct
     2341 tggcgtcctc gaactcgtgc tgggtgacga gccgcttgga gcgacgcgcg gcgagcaacg
     2401 ccgcctcgtt cacgaggtta gcaagatcgg cgccggagaa cccgggcgtc ccgcgcgcaa
     2461 tgacgtgaag atcgacgtcc ggtgccagcg gcaccttgcg cacatgcact ttgagaattt
     2521 tctcgcggcc gatgatgtcc ggacgcggca cgaccacttg gcggtcgaag cggccgggcc
     2581 gcaacagcgc cggatcgagc acgtcaggcc ggttggtcgc ggcgacgagg atgatgcctt
     2641 cgttcgcctc gaagccgtcc atctcgacaa gcaactggtt cagcgtctgc tcgcgctcgt
     2701 cgttaccgcc gccgaggcct gcgccacggt gacggccgac agcatcaatc tcgtcgataa
     2761 agatgatgca cggcgcgttc ttcttcgcct gatcgaacat gtcgcgcacg cggcttgcgc
     2821 ccacgccgac gaacatctcg acgaagtcgg aacccgagat ggtgaagaac ggcacgtttg
     2881 cttcgccggc gatggcgcgc gccagcagcg tcttaccagt gcccggaggg ccgaccagca
     2941 gcgcgccgcg cgggatcttg ccgccgagct tctgaaactt ctgaggatcg cgtaggaact
     3001 ccacgatctc ttgcagatct tccttggcct cgttaacgcc ggccacatcg tcgaaggtga
     3061 cgcggccatg acgctcggtg agcagcttcg ccttggactt gccgaagccc atggcacggc
     3121 caccgcctga ctgcatctgg cgcatgaaga atatccacac cgcaatcagg agtagcatgg
     3181 ggaaccaaga gatcagcaca ccgaacagcg acggcacgtc ctcctcggac ggcttggcgg
     3241 tgatcttcac gcccttggtg ttcagctttt cgaccaggtt cggatcgttc ggcgcgtagg
     3301 tctggaaatt ccggccgtcg gtgtaatgcc cggcgatctg attgccggcg atggtcacgt
     3361 cttggacgtt cccggcatcg acctccgaga gaagctgcga gaagctgatc tcgttaccgc
     3421 ggacctgagc gccttgggta ttgaagaggt tgaaaagtgc gaccagcagc aggccgataa
     3481 acacccaaat gacgaagtta cggaaattcg aattcatgca ctgacctgac ggcttgatct
     3541 caaagacacg ccatggggtg agaaatcccc caaaattgcg gcgcagaaag gcttactagg
     3601 ccctactaac ataagaacgg gcactacttt tgccaagtaa agctaacgcg aagaaatgca
     3661 cgcttcctca atatcccggg cctttcgtcg cgcccagccc cgtgaggaac cttgcccggc
     3721 agtcaaggcc ctcttcatgg cgcgacagtg gcgtcaagaa tggctcaacc tgaccgaaat
     3781 caggcaggca aaggagcctc ctcccccgcc agcaggccgg cagcgcctgg gccgccgggc
     3841 ccggcaagga cgccggcatg gcctctcgct ctcttaggac gcgcaccccc tcctcaccca
     3901 gagcgcggac gataatgggc gcttcctccg cggccccgag ctcgatggca aagcggttgt
     3961 cccacaaggt ccgctcgcct gggctcagct ccagctccgg caggccctcg tgacgcatct
     4021 cgcggaagac gaacagacgc ccgcccttcg gcgcgatacg gcagcgggcc agcgtatgcg
     4081 ccttgtccgg cgactcttta agtgcggcgt atagcgcctc gagcttggcg agccgcaccg
     4141 gctccatccc gccgccaact gtggcgagga ggcgggacag gacgcgaagg gcgacctcct
     4201 ccggcaaagc gtcagttgcg cctgcgtcca ctgacgcata acccgccgcg ctggtctcgg
     4261 cgtggccgtc gagaaagtca caagccgccg catcgagcgc ggcccgcgcc cggcgcaacc
     4321 gcttggcgga cagcgcgatc acatctgcac tgagcccaaa cccggcgagc gtctccatgg
     4381 cgctgcgcac ccgcccgcgc tcgaactttg gatcgatgtt ggatgggtcg gtgacgtagg
     4441 cgatgccggc cgcctcgacc gttgccacga gccgcgcctt cggcacatcg agtagcggcc
     4501 tgagcaacgt cagcccggcc cacgagcctt cctccggaat ggcggcaagg ccgtcgagcc
     4561 cgctgccgcg cgcgagtcgc atcaagaacg tctcggcttg gtcgtcgaga tgatgcgcgg
     4621 tgacgagcgc gccgatgtcg tgcgcatgag tataggccgc catcagatcg taacgggctt
     4681 cccgcgcccg cacctgaagg ccctcgtcca ccccatcatg ctcccaggtg aggatgacat
     4741 gcaggaggct tgctgcttcc gccgcgcgcg acaccagcaa cgcttcgtcg cgcgagtccg
     4801 tacgcaagcc atgatcgacc gtgagcacga cgagttcagg cccgtcgctt cgcgccgcac
     4861 gccactgcgc ggtcaaatgc atgagcgcaa gactgtcggg cccaccggag acggcgagcg
     4921 cgacgcgcgg gagatgcgcg agcggcgcga acagcacctc ggcgtcgtcg tgcgtgacag
     4981 gcgccgccgt catgggcagc ccgccttccc cgccgcggtc ttcgcctgcc cccgctgcgc
     5041 gctattggga aacctggcgc cgagcttctt aaaggtggcg cacgcggcct ccgtcttgcc
     5101 gagctccgca agcgacatgc cgagccggag gagcgtatcg gacgccttat cgctcccgga
     5161 atatttgcgg tagccgtcga ggaaactgtc ggctgccttc tggtactgct tgcgcagata
     5221 ctggctctcg cccacccaat attgcgcggg gctggccttg tccggcagcg acccggtcgt
     5281 gatcgtgctg gatggcgtgg cctgatcgtc gcttggctgg ggcggcggcc cataccagcg
     5341 cggcttcgac ggatcggcaa ttttcgccgg cggcggaacc acaggtgcga gcgagggcgg
     5401 aatgggcacg tcctcgtcct gcggctctgg ctcgggcttg agctcccgct ttgcctcaac
     5461 gatcggcggc ttccgctccg gtgcgggaac cttgggatcg gcaaccttcg gctcggcaat
     5521 cttcgtctcg ggaaccttgg gctcaggaac gttcggctta ggcgccgtcg ccgcgcgcgg
     5581 cctcggctca ggatcggtaa tcatcgccgg ggcgtctttt ttggcggtct cgtctttggc
     5641 gactttctct ttggcgcgct ccggtttcgg gtccggtgcg gcaacctttg gcttgggcat
     5701 tgtcttgtcg gcgggcgccg cttcggacat ctcgttgagc ttgctcaaga acgtgtccat
     5761 ctgacgggtc aaggcgccga tctggacctc gaggccacgg acgcgcccct tgaggtcgtc
     5821 gtcaccggcg ggcgtggcat ccacgccatc gggggcaagg ctcggcctgg caatcacggc
     5881 ggcctcgaga gcgcccacca tgatcctcag ggcggagatc tccgcctcaa gcctggccac
     5941 acgcgtggcg acgtcatccg tggtctttga gctttcagtc tcttcggcga ggctcgcgcc
     6001 cattggcagg agcacggcaa gagcgaacgc cgcgagcacg gcacgccata aatgagaacg
     6061 tgagggcacg ggttccttcg accttatcgg catagccagg cgacttaacc gactcgttgc
     6121 cgtggcgttt aggccatgtg tgtggcccac aacgcgccgt ggaacaaggc ctagtagcta
     6181 gcgacggcgc ccccggtaag gaccgtctgc gcccgccggt tccgggacca gcaggagatg
     6241 tcgtcgcaag ccgcgaccgg ccgctcccgg ccataggaga cggtgtgaag acggcccgcg
     6301 cggaggccgt ggcgcacaag gaaggtcttc acggccatgg cccgctgcgc ccccagcacg
     6361 aggttgtgct gccggcttcc cggctcactc gcgtgcccag cgatcatcac ctgatattgg
     6421 gggtgctggt tcagccagcg aacctgctgg cgcaggatgg cgcgcgaatg cccggtcagg
     6481 accacgctat ccccttggaa ataaacgaca tcgcccacat tggcggcgaa gtcctcaggg
     6541 ctgccagggg tggccggagc cgccccgcca aggccaaatc ccttgggctg ctgcggctgg
     6601 cgcgaacaag cgcccgcgct gaaggccaaa atgatcaccg ccacgaccac gcaagcgcgg
     6661 ccgatcccac caaaacgcat gactgaaaac acccaatccc ccaaacccga ggaaatccta
     6721 gggggcggag cttaaggatg ccttagccag gcagatgcgt ggggcgaaat agaaaaatgg
     6781 aggccaaaaa aggaaaagga tgctccaagc gccgatatct ccagcgccct gagcatccca
     6841 aatcccgtgg ggggaaaccc gaaagtttcc cgaagccgtt gcttagccga gcttcagcgg
     6901 ctcaacgata acggccttct cgtgatggcc aaagcaacca ccaagcgaaa cggcggcagc
     6961 caccagcatt acagcggcga taacgccttt caaaccctgc atggtcttct ctcctttggt
     7021 taaaacggca atagccgtct ttgcatttac cggtgcggct caatcggatc aagccaggac
     7081 tgcagtcccg agaccgattt ggcgatcaat gatgcgaaaa tgccacgccg aatctcatcc
     7141 tccagctttt gcaagctatc ccccacgcgg acgccgatca agatgtggtt aacgacgttg
     7201 gggcactcgg attaacaaaa ggtaagcgac tgtggcaaat aagccgcaac ccgcacgggt
     7261 aaaccgacaa ccgctcggga atatgccgaa aaatcaggca ctgatcgcgt ggcaaaagcc
     7321 aaatcgagcc aattttgacc ccagaaacaa ccctcggttg cgccgcgtca gattgcgtcg
     7381 tgagagatgc gcgaaatcca agaagaacgt gacagcgagg cctttggggc cccgccggta
     7441 gcgctgacct cagcgcgggg ccacttggtc ggtgaggagc gtcacgggca gccccgcctc
     7501 cttgaccctg accagcaccc tggcgacatc gccataggcg gattgagtgt cgccgcgcac
     7561 ccagacctgt gccgtaccgc ccgcatcgcg aatcgcggca agcttgggcg caaggtcctc
     7621 aaagctgatc tgcgcgttct gaaggtagag cgtgccgtcg gcctcgatcg tgatgctgag
     7681 cggtgctcgc tccggcgcca cctgtccggc ctccgtcctc ggcaaattga cgtccggcag
     7741 cgaggccttg gcatcgatgt cgacgcccac ggtcagaagc ggcgccgtca ccatgaagat
     7801 gatcaggagc acgagcatca catccaccag gggcgtgacg ttgatctcgc tcatgggcac
     7861 gcgggagcgg cgccgcgcgc ggcggttgga caatgcgcca ccgggttgaa gtgctccgcc
     7921 catccttgcc ctcacagcgt cttgtcgatc tggcgcgaca gaatggccgc gaactcatcg
     7981 gcgaaccctt caagccgcgc cgcgtgccgc ccgacctcag cgttgaactt gttgtagaag
     8041 atgacggccg gaatggcggc aagcaggccc agcgcagtcg caaacaaggc ctccgcgatg
     8101 ccgggcgcca cgaccgcaag ccctgtattc ttggtggtcg cgatcgcctg gaagctcgtc
     8161 atgatgcccc agacagtgcc gaacaggccg ataaagggcg ccgtcgagcc caccgtggcg
     8221 aggaacgtca gccgccgttc gagccgttcg acctcacggg aaacggtcac gtccatgacc
     8281 ttctcgatgc ggtgctgcat gcccggcatg ggcttcgtgg accggtcgcc gctgtcgcgc
     8341 tccgtcgatc gcttccactc acgcatcgcc gccacgaaca gcgccgccat gcccgtgttc
     8401 cgccgctggg cgagggcctg atagagctca tcgagcgact ggcccgacca aaacacctgc
     8461 tcgaacttgt ccgcctcttt gcgcgtcttg gtgaacagga agaacttctc gataatgatc
     8521 gcccaactcc acaccgaggc ggcaagaagc ccaacgatga cgatctggac aacgatatgg
     8581 gcgttcttga acagctccca aatcgacaga tcgacggcga catccgaatt cattcacggc
     8641 cctcgtggca tcaacgccaa aagctggcac ctttcggcac cggctcttgg ccccgcgagg
     8701 cgcaccatcg cgggctattc atcgttaacc ggcagcggcc caaaaacact ataaaatcaa
     8761 gccattcggc ttgacccaag cgtcaaattt gggcggccac cgccggcaca aaatcctgtt
     8821 ccgccctctt cgctaagttc aacttgctta cttaacgaat cgttcaagtg ccccgcggac
     8881 caactgtccc agccgttggg gcttgcccgc ctccgagacc agcaccacca cgaccttggc
     8941 ccgaactagg acgacgccgt cgcgccggac ctcttgggac agcgtcaggc tcgccgcgcc
     9001 ggtctccacg caagcggtga ccacttcgag cacatcatcg agcttggccg gcttcagata
     9061 atcgatctcc acgcggcgca cgacgaagac ggatgcctcg ccctgatcgg gattggcgag
     9121 gtcctggtga tggattccaa gcaggcgaat aaagtcagag cggccccgct cgcagaactt
     9181 cagatagttg gcgtggtaga cgaggccggc gaagtccgtg tcctcgaaat agacgcgcac
     9241 cggcaggata tgggtgtcgc cctcgatgcg tccggcaagg tcgggccaac tactcactct
     9301 gcctcgccgc cattctcacc gccgttgaag agcgggaact gcgacggctg agcgggcggc
     9361 gtgaccccga gatgctcgaa ggcgagcccg gtgagcacac ggccgcgggg cgtgcgcgca
     9421 atgaagcctt gctgcaagag ataaggctca atgatctcct cgatcgcatc gcgcggctcc
     9481 gacaggccag cggcgatggt ctcgatcccc accggcccgc cgccatagtt cacggcgatg
     9541 agcttcaggt agcggtggtc gagcgcatcg agcccgcgcc cgtccacttc tagctgcgac
     9601 agcgcgctgc ccgcgagctt ggcatcgacc gcgccgttgc catccacggc ggcgaagtcc
     9661 cgtacgcggc gcaagagccg cgccgccacg cgcggcgttc cgcgcgcgcg tctcgcgatc
     9721 tcatgggcgc cttcgggcgt cagcggcagt tgaagcagcc gcgcgccacg ccgcacgatc
     9781 tcttccagct cttccaccgt gtagaactcc agccggatgg gaatgccgaa gcgttcgcga
     9841 agcggcgtgg tgagaaggcc cgtccgcgtc gtggcggcga ccagcgtgaa cttggccagg
     9901 tcgatgcgca ccgagcgcgc cgccggcccc tcgccgatga tgaggtcgag ctggaaatcc
     9961 tccatggcgg gatagagaat ctcctccaca gccgggctga ggcgatggat ctcgtcgatg
    10021 aacagcacgt cgcgttcttc gagattcgtc agcagcgcgg cgagatcgcc cgccttggcg
    10081 atgacgggtc ccgacgtcgc ccggaaattg accccaagct cccgcgccac gatctgggcg
    10141 agcgtggtct tgccgagccc cggaggcccg gcgaacagca catggtccag cgcctcgcgc
    10201 cgcgaccccg ccgcatcaat aaagactttt aggttggagc ggaccttctc ctggccgatg
    10261 aattcggcca gtgtttgcgg ccgcagcgcc gcctcctgct ggtcgtcgtc tttcttcgct
    10321 gctgccagga cccgctcgct catcctgcaa gctccttgag gccaatacgg atcagctctt
    10381 ccgccttcgc ctcgttgccg gcgacaccaa ttgccgagga tacggccgcg gccgcctcac
    10441 catgggcata gccgagattg gtcagcgcgg agaccgcatc acgcgcggcc aagccttcgg
    10501 gcaggatggc gatgggcgca gggcctgagc ccggtgccat ggcgggcgcc agcgctggca
    10561 tcttgtcctt catttcggcg acgatacgcg cggcgacctt ctgcccgacc ccttgggccc
    10621 gcgccacttg cgccttgtct tgcagcacca ccgcgttggc gagatcggcg gc
//
LOCUS       DBNHVU010000097         6955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1821893, whole genome shotgun sequence.
ACCESSION   DBNHVU010000097 DBNHVU010000000
VERSION     DBNHVU010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6955
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1821893"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="AC16Y7_03745"
     CDS             <1..568
                     /locus_tag="AC16Y7_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708527.1"
                     /translation="TVALAGTLACASLLGGCGGVEFEGKVFDYMGMSGSRQQADVQMS
                     ERPPLMVPPNVQQLPAPTQSPAAVSTARQDWPDDPEKVRVRVIEQENTKKAEAQAAAD
                     PLNPYAGKETLLDKLLGGDKTVEEPVPNVPAPDPSDRAPTVAQTRSAPNTPHVPQAPL
                     PSQNMEEFKPAAPDSYNRASQGRAMGEY"
     gene            819..2165
                     /locus_tag="AC16Y7_03750"
     CDS             819..2165
                     /locus_tag="AC16Y7_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR5708528.1"
                     /translation="MPVAAVAEEAKSERVSEFTLANGLQVVVIPDHRAPVATQMVWYK
                     AGAADEPPGTSGIAHFLEHLMFKGTDNIQQFSKIIAKNGGDDNAFTNHDVTAYFQRVA
                     KDRLPKVMEMEADRMANLRLTPEDVATERDVILEERRSRVDNDPGAILQEQMMAALYR
                     NHPYGIPIIGWEHEMEELDREDALAFYRRFYAPENAILIVAGDVEPDDVKALAEKTFG
                     KIPRNGEAQQRARPSEPEHDAPLTVTLEDPRAGRTTVQRYYIAPSYASAEPGEAEALD
                     LLMRVAAHGATSRIYKQLVMKDHVAASAGGWYSDSGLDSGRLGFYAIAGPKTAPEEIE
                     AAIDRTVEDLRENGVTQAELDRARASQIAEFVYSSDSISKMARQYGWRLAAGMTIEDV
                     EAWPQRMKQVTVDDVRAVARKYLDEENSVTGILKPEPEHTSSAGEKPAPAAGNGKS"
     gene            2274..3545
                     /locus_tag="AC16Y7_03755"
     CDS             2274..3545
                     /locus_tag="AC16Y7_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR5708529.1"
                     /translation="MKIQEVTSPGGIKAWLVESHANPLITIRFAFFGGASQDAPSREG
                     QAYFVTAMMNEGAGDLDAIAFQEREEALAMKMDFDAGRDVMLGRIQTLTENKDDVFDL
                     VRLAMSQPRFDEDALERVRAQILAGLKFDENDPETVASLAWDRIAFQDHPYGRPVKGT
                     KDSVAEISADDLRDYVRRVFARDKLAVAVVGDIDAEELGEVLDKLFGDLPERSDLVQV
                     ADAPPPLGPTREVIAMDVPQSVAQFGLRGIPRDDDDFIPAYVLNYIIGGGGFSSRLME
                     EVREKRGLAYSVRSSLYPYMHGPVFIGHVATKNEAVGQSLSVIQNELERIAEQGPTAE
                     ELESAKAYLTGAYPLRFESSVSIASQLLWIQIEDLGIDYVDRRNALVDAVSMDDIKRV
                     AKRLLTPDRLITTIVGKPVEPAEVVPAGAPG"
     gene            3634..5436
                     /gene="mutL"
                     /locus_tag="AC16Y7_03760"
     CDS             3634..5436
                     /gene="mutL"
                     /locus_tag="AC16Y7_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR5708530.1"
                     /translation="MAIRQLPPNLINRIAAGEVVERPASVVKELVENAIDAGAARIDV
                     TVTGGGLSLIRVADDGAGMAAEDLALAVERHATSKLAGDNLSHILSLGFRGEALPSIG
                     AVARLAITSRAENAKDAHTIAVDGGRKAPLKPAARAGGTDVEVRDLFYATPARLKFMK
                     SERAEAAAISDAVKRLALAHPEIAFSLSTGERTTLRLEACPPGLLDHALARLGRIMGA
                     EFVQDALPVSAERGTMRVEGFAGLPTLHRPNGMQLYLVVNGRPVRDKLLAGTVRAAYG
                     DLVPRGRYPMLALFLDVPPEEVDVNVHPAKTELRFRDANAMRSLLIGAIREALSAAGH
                     RATESLSQEALQRMAPPSVGSSAQAPVPSYRSHTRPRDVGFAERSQAPLAEVSVPSAD
                     MRGAATEEETATDFPLGAARAQVQDTFIIAETDDALVIVDQHAAHERLVYERLKRAFA
                     NGGIARQMLLIPEVVELDADAAQALLAASDALERLGLVIETFGPSAIAVREVPGLLGT
                     CDVKGLLAELASTLLEAEDGEAGRLLAERLDYVLSTMACHGSVRAGRRLSPEEMNALL
                     RGMEETPFSGQCNHGRPTYVELKLTDIEKLFDRR"
     gene            complement(5440..5934)
                     /locus_tag="AC16Y7_03765"
     CDS             complement(5440..5934)
                     /locus_tag="AC16Y7_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708531.1"
                     /translation="MTLRALFFAPLILLAATAGQDRFTSGVWQGNANYDDEGYFSDCT
                     MTAQAEKDILLGFVMSKDLDWGLVIADETRTFEVGARKAVLLIVDDREPMAAIAKVID
                     VHGILVPLKSSDTVLDAMRHGKVLRIASDQTEFSFQLTGTRDAIAALTQCVTAHQGTN
                     RVQL"
     gene            complement(6001..6561)
                     /gene="rsmD"
                     /locus_tag="AC16Y7_03770"
     CDS             complement(6001..6561)
                     /gene="rsmD"
                     /locus_tag="AC16Y7_03770"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR5708532.1"
                     /translation="MRIIAGRFKGKTIDAPKGLGTRPTSDRVRESLFNVLEHGAPGLD
                     FAGVRALDLFAGSGALGLEALSRGARYALFIDDDTAARAAMRRNIEALGLTGVTKIWR
                     RDATKLGPAGNITPFSLVFCDAPYGKGLSAQALEGAAEGGWLEPGAVAVWEEHAGAEV
                     AWPAPFTELDRRRYGATEIAIAEFTQ"
     gene            complement(6561..>6955)
                     /locus_tag="AC16Y7_03775"
     CDS             complement(6561..>6955)
                     /locus_tag="AC16Y7_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pseudouridine synthase family protein"
                     /protein_id="HIR5708533.1"
                     /translation="GALARHLELPSTGWVRRYRVRAHGRVRQDALDRLKSGVTIDGVR
                     YGPVEAKLERQQGSNVWLTVGLHEGKNREVKRIAEHLGLTVNRLIRTSYGAFSLGDMA
                     QGAVEEVKRKVLIEQLGTARAREFGVKR"
BASE COUNT         1239 a         2281 c         2226 g         1209 t
ORIGIN      
        1 aacggtcgcg ctcgcgggca cgcttgcctg cgcgagcctc cttggcggct gcggcggcgt
       61 cgagttcgaa ggcaaggtct tcgactatat gggaatgtcc ggcagcaggc agcaggccga
      121 cgtccagatg agtgagcgcc cgccgctcat ggtcccgccc aacgtccaac aattgccggc
      181 gccgacccaa agccctgccg ccgtgtccac ggcccgccag gattggccgg acgacccgga
      241 gaaggtgcgc gtccgcgtca tcgagcaaga gaataccaag aaggctgagg cccaggcggc
      301 cgccgacccg ctgaacccct atgcgggtaa ggaaacgctg ctcgacaagc tattgggtgg
      361 cgacaagacg gtggaagagc ccgtccccaa cgtgcccgcg ccggatccgt ccgatcgggc
      421 gcccactgtc gcccagactc ggtccgcgcc caacacaccg catgttccgc aggcgccctt
      481 gcccagccag aacatggaag agttcaagcc tgcggcgccc gatagctaca acagagcctc
      541 ccaagggcgg gccatggggg agtactagag ttcgttcgtc accttttcgc cggccggttc
      601 gcgcgtcggt cgcaatctag cgcttccatt tcttgaaaaa cccggcctga ggcccgacat
      661 gaggcattgg cgctagcagc ctactaggct tattttcccg cgcgccgtga gccccgtatt
      721 ccaatccaaa ggccttcgtt cgaatggtgg tgtggatgca agatcgattg accgccttcc
      781 ttcgtgttgg cctaattcta gccagtgtga gcctttcaat gccggttgcc gccgttgccg
      841 aagaagcgaa atcagagcgc gtcagcgagt tcaccctcgc caacgggctc caagtcgtcg
      901 ttatccccga tcatcgcgcg ccggtcgcga ctcagatggt ttggtacaag gcaggggccg
      961 cggacgagcc gccgggcacc tccgggatcg cacacttcct tgagcatttg atgttcaagg
     1021 gcaccgacaa catccagcag ttctccaaga tcattgccaa gaatggcggt gacgacaacg
     1081 cgttcaccaa tcacgacgtg accgcgtact tccagcgcgt agccaaggac cgtcttccca
     1141 aagtcatgga gatggaagcc gaccgcatgg caaatcttcg gctgacaccg gaggacgtgg
     1201 cgaccgagcg ggacgtcatt ctcgaggagc gccgctcgcg cgtcgacaac gatcctggcg
     1261 ccatcctcca agagcagatg atggccgcgc tttatcgcaa ccatccctac ggcattccca
     1321 tcatcggttg ggaacacgag atggaggagc tcgaccgcga ggacgcgctc gccttctatc
     1381 gccgctttta cgcgccggag aacgccatcc tgatcgtcgc tggggacgtc gagccggacg
     1441 acgtaaaggc gcttgccgag aagaccttcg ggaagatccc gcgcaacggc gaggcgcagc
     1501 agcgggcgcg tcccagtgag ccggagcacg atgcgcctct cactgtgacg ttggaggatc
     1561 cgcgcgccgg ccgcaccacc gtgcagcgct actacatcgc gccgagttac gcctcggcgg
     1621 agcccggcga ggccgaggcg ttggatctgc tgatgcgcgt cgccgctcat ggcgccacca
     1681 gccgcatcta caagcagctg gtaatgaagg accatgtcgc tgcgtccgca ggcggctggt
     1741 actccgactc cgggctcgat tcggggcgct tgggtttcta tgccattgcc ggaccaaaga
     1801 ccgcgcccga ggagatcgag gcggcgatcg atcgcacggt cgaggacttg cgcgagaacg
     1861 gcgtcaccca agccgagttg gaccgggcgc gcgcctctca gattgccgag ttcgtctaca
     1921 gttccgactc catctccaag atggctcgcc aatatggatg gcggctggcc gccggcatga
     1981 ccatagagga cgtcgaagcg tggcctcagc ggatgaagca ggtcaccgtg gacgacgttc
     2041 gcgccgtggc ccgcaaatat cttgacgagg agaactcggt gacgggcatt ctcaaaccgg
     2101 agcccgaaca cacaagcagt gccggcgaaa agcccgcccc agctgctgga aacggcaagt
     2161 cgtagatgcg gaccaggatt tgctcttcgc tcttcgccgc aacgctgtgg ctcgggacga
     2221 cactgctggc ggttgcgctt ctggcgtcgc ccgccgcggc caaagggagt ggcatgaaaa
     2281 tccaagaggt gacgagccct ggcggcatca aggcctggct cgtggagagc catgcaaatc
     2341 cgctgatcac gatccgtttt gccttcttcg gcggtgcatc gcaagacgcg ccaagcagag
     2401 aaggccaagc ctatttcgtg acggcaatga tgaacgaagg cgccggtgac ctcgacgcca
     2461 tcgcgttcca agagcgcgag gaagcgctgg cgatgaagat ggacttcgac gccggccgcg
     2521 acgtcatgct cggcaggatc cagacgttga ccgagaacaa ggacgacgtc tttgacctgg
     2581 tgcggctggc gatgtcgcag ccccgcttcg acgaggacgc actggagcgc gtgcgcgcgc
     2641 agatcttggc tggactgaaa ttcgacgaga acgatcctga gaccgtggcg tcgctcgcct
     2701 gggaccggat cgcgttccag gatcatccct atggccgccc cgtgaagggc acgaaagact
     2761 ccgtggctga gatctccgcg gacgatctca gggactatgt ccgccgcgtg tttgcccgcg
     2821 acaagcttgc cgtcgccgtg gtcggtgaca tcgacgccga ggagttgggc gaggtgctcg
     2881 acaagctgtt cggcgatttg ccggagcgct cggacctggt gcaggttgcc gacgcgccgc
     2941 cgccccttgg gcccacccgc gaggtcatcg ccatggatgt gccgcagtcg gtggcgcagt
     3001 tcggcctgcg cggcatcccg cgcgacgacg acgatttcat tccggcctac gtgctcaact
     3061 acatcattgg cggtggcggg ttctcctcgc ggctgatgga agaggtgcgc gagaagcgtg
     3121 gccttgctta ctctgtccgc tccagcctct acccctatat gcacggcccc gttttcatcg
     3181 gccacgtggc tactaagaac gaggccgttg gccagtcgct gtccgtgatc cagaatgagc
     3241 ttgagcgcat cgcggagcag ggtcccacgg cggaggagct ggagagcgcc aaggcctatc
     3301 tcaccggcgc ctaccccttg cgcttcgaat cctctgtaag catcgccagc cagctcttgt
     3361 ggatccagat cgaggatctt ggcatcgact atgtcgacag gcgcaatgcc ctcgtcgatg
     3421 ccgtgagcat ggatgacatc aagcgcgtgg cgaagcgcct gctcacgccg gaccggctga
     3481 tcaccacaat cgtcggcaag cctgtggaac ctgcggaagt cgtgcccgcg ggcgcgccag
     3541 gctaggccct ccacacctag aatcggctta agctaaaggc cccaactgtt ggggcagcgc
     3601 taagcggaat caggccaaaa gcggaggcag actatggcca ttcgtcagct gccgccgaac
     3661 ctcataaatc gcattgccgc gggtgaggtc gtcgagcggc ctgccagtgt cgtcaaggag
     3721 ctggtggaga acgcgatcga cgcgggcgcg gcccgtatcg acgtgacggt cacgggcggc
     3781 gggctgtcac tgatccgcgt ggccgatgac ggcgccggca tggctgcgga ggacttggcc
     3841 ctggcggtcg agcgccatgc cacctccaag ctcgccgggg acaacctctc tcatattctg
     3901 tcgctcggct ttcgcggcga ggcgctgccc tccattggag cggtggcgcg gcttgccatc
     3961 accagccgtg ctgaaaacgc caaggacgcc cacacgatcg cggtcgatgg gggccggaaa
     4021 gcgccgttga agccggcggc gcgcgcgggc gggacagacg tggaggtgcg cgatctgttc
     4081 tacgcgacgc ccgcgcgtct aaaattcatg aagtctgagc gcgccgaagc tgcggccatc
     4141 tccgacgccg tcaagcggct ggcactggcg catccggaaa tcgccttctc actatcgacc
     4201 ggcgaacgaa ccacgttgcg cctggaggcc tgcccgccgg gcctcctcga tcacgcactc
     4261 gcgcggctcg gccgcatcat gggcgcggaa ttcgtgcagg acgcgcttcc cgtctccgcc
     4321 gagcggggga caatgcgcgt cgaaggcttt gcaggcctcc ccacgcttca ccgccccaac
     4381 gggatgcagc tctatctcgt ggtgaacgga cgccccgtgc gcgacaagct cctcgctggg
     4441 acggtgcgtg cggcctatgg cgacctcgtg ccgcggggcc gctatcccat gctggcgctt
     4501 tttctggacg tgccgccgga ggaggtggac gtgaatgtcc atccggccaa gacggagctg
     4561 cgcttccgcg acgccaacgc catgcgctcg ctgctcatcg gcgcgatccg ggaggccttg
     4621 agtgccgccg gtcaccgcgc cacggagagc ctgtcgcagg aggcgctgca acgcatggcc
     4681 ccgccatccg ttggctcaag tgctcaagcg ccggtgcctt cctaccggag ccacacgcgc
     4741 ccgcgcgacg tgggcttcgc ggaacggtcg caagccccgc ttgcagaagt ttctgtgccc
     4801 tcggccgata tgcgcggggc cgctacggag gaggaaacgg caaccgactt cccgctgggt
     4861 gccgcccgcg cgcaagtgca agacaccttc atcatcgccg agacggacga cgcgctcgtc
     4921 atcgtcgatc agcacgcggc ccatgaacgc ctcgtctatg aacggctgaa gcgcgccttc
     4981 gccaatggcg ggatcgcgcg gcagatgctg ttgatccctg aagtcgtcga gctcgatgcg
     5041 gacgcagccc aagccttgct tgcggcgtcg gatgcgcttg agcggctcgg tcttgtcatc
     5101 gagacgtttg ggccttcggc gattgcggta cgcgaagtgc cggggttgct cggcacctgc
     5161 gatgtcaaag gactgcttgc cgagctcgca agcacactcc tcgaagcgga ggacggcgag
     5221 gccggccggc tgctcgccga gcggctggac tatgtgcttt ccaccatggc ctgccacggt
     5281 agcgtgcgcg cgggccggcg tctgtcgccg gaggagatga acgcgcttct gcgcggtatg
     5341 gaggagacgc cgttctccgg ccagtgcaat cacggccgtc cgacttacgt cgagctgaaa
     5401 cttaccgaca tcgagaagct gttcgaccgg cgctaggtgt tacagctgga cgcggttcgt
     5461 gccttgatgc gcggtgacgc attgcgtcag cgccgcgatg gcgtcgcgcg tgccggtcaa
     5521 ttgaaagctg aactcggttt ggtcgctggc aatcctgagc accttgccgt ggcgcatggc
     5581 gtcgagcacg gtatcggaac ttttcagcgg aacgaggatg ccgtgaacgt cgattacctt
     5641 ggcaatcgcg gccatcggct cgcggtcgtc cacgatcagg agcacggctt tccgggcgcc
     5701 gacctcgaac gtgcgggtct cgtcggcaat caccagcccc cagtcgaggt ccttgctcat
     5761 cacaaagccg agcaagatgt ccttctcggc ctgcgcggtc attgtgcagt cggagaaata
     5821 cccctcgtcg tcgtaattgg cgttgccttg ccagacgcca gaggtgaagc gatcctgtcc
     5881 ggccgttgcg gccagcaaga tcaacggcgc aaagaataag gctcgcaacg tcatgagact
     5941 ccccgaaagc atgtcgcgac ccatctaatc atgtttcgac agttgcgcga aaaagcccgt
     6001 ctattgggta aattcggcga tggcgatctc cgtggcgcca tagcggcgcc ggtcgagctc
     6061 cgtgaacggc gcgggccacg cgacctccgc gcccgcgtgc tcttcccata cggcaacggc
     6121 gcccggctcc agccagccgc cttccgccgc gccttctagt gcctgtgcgc tcagcccctt
     6181 gccgtaaggg gcgtcgcaaa agacgaggct gaacggcgtg atgttgcctg ccgggccgag
     6241 cttggtggcg tcgcgccgcc agattttcgt gacgcccgtc agccccaacg cctcgatgtt
     6301 gcggcgcatc gcggcgcgcg cggccgtatc gtcgtcgatg aagagcgcat agcgtgcgcc
     6361 acgggacaga gcttccaggc ccagcgcgcc ggagccggcg aacaggtcga gcgcgcgcac
     6421 ccccgcaaag tcgaggcccg gcgcgccgtg ctccagcaca ttgaacaggg attcgcgcac
     6481 gcggtccgat gtcgggcgcg ttcccagtcc cttcggggcg tcgatggttt ttcctttgaa
     6541 ccgcccggcg atgatgcgca tcagcgtttc accccgaact cgcgggcgcg tgctgtaccc
     6601 aattgctcaa tcagcacttt gcgctttacc tcttccacgg cgccttgcgc catatcgcct
     6661 aatgagaacg ccccgtacga ggtgcggatc agccggttca ctgtgagccc caaatgctcg
     6721 gcgatacgtt tcacctcgcg gttcttgcct tcgtgcaaac ccacggtcag ccacacattg
     6781 ctgccttgct ggcgctcgag cttggcctcc accggcccgt agcgcacgcc gtcgatggtc
     6841 acaccggact tcaagcggtc gagcgcgtcc tggcgcaccc gcccatgcgc gcgcacccgg
     6901 tagcgccgca cccatccagt gctcggcagc tccagatgcc gggccagcgc cccat
//
LOCUS       DBNHVU010000098         5070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_263371, whole genome shotgun sequence.
ACCESSION   DBNHVU010000098 DBNHVU010000000
VERSION     DBNHVU010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_263371"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..144
                     /gene="rpmH"
                     /locus_tag="AC16Y7_03780"
     CDS             10..144
                     /gene="rpmH"
                     /locus_tag="AC16Y7_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR5708534.1"
                     /translation="MKRPYQPSRIVRKRRHGFRARMATKAGRKVLARRRAKGRKRLSA
                     "
     gene            216..557
                     /gene="rnpA"
                     /locus_tag="AC16Y7_03785"
     CDS             216..557
                     /gene="rnpA"
                     /locus_tag="AC16Y7_03785"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR5708535.1"
                     /translation="MRGGTRFTTPSLVLQARRSRAGSPATPTQARFGFTATKSLGGAV
                     VRNRARRRLKEAVRLTGSAHASEGYDYVLIARGGTVQRRFTDLIKDLERALAKVHEPS
                     SAKRRRRPKSD"
     gene            605..2596
                     /gene="yidC"
                     /locus_tag="AC16Y7_03790"
     CDS             605..2596
                     /gene="yidC"
                     /locus_tag="AC16Y7_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIR5708536.1"
                     /translation="MDENNRNFIFAIVLSMAVLFAWQFFFVPETPPEGQQAAQKAEQG
                     LPTPSADSAPPQPGAGTAPQPGASTAPQPEGSPTGLTRKTALAASPRVEIDTPSVRGS
                     ISLKGARLDDLTLKDYRVTVDPNSPQVILLSPAGGPHAYYAERGFVGAGGNDLGLPTG
                     DTLWTAEGQGRLTPATPVTLAYDNGKGLKFTRTISVDDKYMFTIDDTVTNTGTDAVTL
                     YPYGLVSRHEFPEVEGFYILHEGLIGVVDDGLEEISYSSAIDDPPATFKSKNGWLGIT
                     DKYWATVVVPEQNQSFDAKFAGTQNGDRTRFQTDYLMGPLTVAAGGTEQVSGRVFAGA
                     KEVNVVDGYASTYDIPKFDLLIDWGWFYFLTRPLFYALDYFYKLVGNFGVSILIVTVL
                     IKLVLFPLANKSYVSMSRMKKLAPEMQKIKERYGEDRMRQQQAMMELYKKEKVNPASG
                     CLPILVQIPVFFALYKVLFVTIEMRHAPFFGWIKDLSAPDPTSVFNLFGLIPWDPPTF
                     LMIGAWPLIMGFTMWFQMKLNPAPPDPMQQKLFTWMPVIFTYLLASFPAGLVIYWAWN
                     NSLSILQQSFIMWRQGVDIPLLENLGIKPSHGAADENEEAKSAKTEAAQKRSAKAARR
                     AKRAAKQTNSGAAKKASAKKASAKKAEDAETDGGEDRPA"
     gene            2648..3307
                     /gene="yihA"
                     /locus_tag="AC16Y7_03795"
     CDS             2648..3307
                     /gene="yihA"
                     /locus_tag="AC16Y7_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIR5708537.1"
                     /translation="MDHPEFTADEIARGEELFKAPSEFVKGVVSIAALPDEGTPEIAF
                     AGRSNVGKSSLINALTSRKTLARVSVTPGRTRELNFFTLGPESELYLVDMPGYGYARA
                     PKSEVKGWTQLIRDYLRGRRQLKRVFLLIDARHGIKANDRDTMKLLDEAAMSYQAVLT
                     KADKPKQSELQAVIAKTEDELSKRPAAYPQILVTSARTGSGIPELRAAVATLAPNGVV
                     P"
     gene            3333..4328
                     /gene="argB"
                     /locus_tag="AC16Y7_03800"
     CDS             3333..4328
                     /gene="argB"
                     /locus_tag="AC16Y7_03800"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR5708538.1"
                     /translation="MAKTKTAQVKSKDPAPPKDAGPLADVLASVHANAKTLSEALPYM
                     QRYDRKTVVVKYGGHAMGDPELARDFARDIVLLKQAGVNPVVVHGGGPQIGKLLERLN
                     IKSEFKDGLRVTDRETVDVVEMVLAGSVNKEIVSAINNQGGKAVGICGKDANLMRAKR
                     LERRVRDPQSNIEQVLDLGFVGEPGLVEPHIMDVIIESDLIPVVAPIGVGPDGETLNI
                     NADTFASALAVALKAKRLLLLTDVDGVLDKEGRLVRSMSTAEAQSLIDDGTINGGMIP
                     KIEGCVDVISEGVEAVVIINGKVPHAVLLELFTEHGVGTLIERKRPGGSRTRAPR"
     gene            4325..>5070
                     /locus_tag="AC16Y7_03805"
     CDS             4325..>5070
                     /locus_tag="AC16Y7_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HIR5708539.1"
                     /translation="MSATQGQGFGHVKTWVFDLDNTLYPADCDLFSQIDKRMGAFIAD
                     AFDLSIEDAHALRQRYYFEHGTTLAGLIRLHGVCPDSFLDYVHDIDLDVVAPSPELVA
                     AIDALPGRKFVFTNGSRKHAEAVAEKLGVLDHFEDIFDIRALDFVNPKPTREAFDRFT
                     GTHGIETANAAMFDDLPHNLETAQAIGMTTVWIDCRATGRPEHQSHETWSTLPDHIHH
                     RTEALAPFLTAIASALAEDAESPATRLPQA"
BASE COUNT         1001 a         1662 c         1568 g          839 t
ORIGIN      
        1 agaagatctt tgaagcgccc ctaccaacca tcgcggatcg tccgcaaacg gcgtcacggt
       61 tttcgcgccc gtatggcgac caaggccggc cggaaggtgc tcgcgcggcg cagagccaag
      121 gggcgcaagc gcctgtcggc ctaaggagcg gcggatatcc gcctcacccg gtgacggcca
      181 tcggacgtct caagacccgg ccagaatttt tgtatgtgag gggcggcacg cgcttcacca
      241 cgccttcgct tgtgctccag gcacgccgca gccgcgcggg ttcgcccgct actcccaccc
      301 aagcccgctt cggctttacc gcgaccaaaa gcctcggcgg tgcggtcgtg cgcaaccggg
      361 ccagacgacg cctcaaggag gcggtgcggc tgaccggctc agcgcacgcc tcggaaggct
      421 atgattatgt gttgatcgcg cggggaggca cggtacaacg gcgcttcacc gatctgatca
      481 aggatcttga acgcgcgctg gccaaagtgc acgaaccctc ttcggccaag aggcgccgca
      541 ggcccaaatc cgattagaaa cttccacacg cttggcgaag aagcggcgtt gaactgaaag
      601 cgacatggac gagaacaacc gtaactttat cttcgccatc gtgctgtcga tggctgtgct
      661 gttcgcctgg cagttcttct tcgtgcccga gaccccgccg gaggggcagc aggccgcgca
      721 gaaggccgag caaggactgc ccacgccttc ggccgacagc gccccgccgc agccaggcgc
      781 cggcacagcg ccgcagccag gcgccagcac ggcgccgcag ccggaaggct cgccaacggg
      841 gctgacgcgg aagacggcgc tcgccgcctc gccccgcgtt gaaatcgaca cgccgagcgt
      901 acggggctcg atctcgctga aaggcgcacg cctcgacgat ctgacgctca aggactatcg
      961 cgtcaccgtg gacccgaaca gcccgcaggt cattctcctg tcacctgccg gaggaccgca
     1021 cgcctattac gccgagcgcg gcttcgtcgg cgccggcggc aacgaccttg ggctacccac
     1081 cggcgatacg ctgtggacgg cagaaggcca aggccggctc acccccgcca cgcccgtcac
     1141 gctcgcctac gacaacggca aagggctgaa gttcacgcgc accatctcgg tcgacgacaa
     1201 gtacatgttc acgatcgacg atacggtcac taacacaggg accgacgccg tcacgctcta
     1261 tccatatgga ctcgtctccc ggcacgagtt ccccgaggtc gagggcttct acattctgca
     1321 tgaaggcctg atcggggtgg tcgacgatgg gctggaggag atcagctatt catcggcgat
     1381 cgacgatcca cccgccacgt tcaagagcaa gaacggctgg ctcggcatca ccgacaaata
     1441 ttgggcgacc gtcgtcgtgc cggagcagaa ccagagcttc gatgcaaagt tcgccggcac
     1501 acagaacggc gaccggacgc gtttccagac cgactacctg atgggaccgc tgaccgtcgc
     1561 ggccggcggt accgaacaag tttcggggcg cgtgttcgcc ggcgccaagg aagtgaacgt
     1621 cgtcgacgga tacgcctcca cctacgacat tcccaaattc gatctcctga tcgattgggg
     1681 ctggttctac tttttgacgc ggccgctctt ctacgccctc gactacttct acaagctggt
     1741 cggcaatttc ggcgtgtcga tcctgatcgt caccgtgctc attaaactcg tgctcttccc
     1801 gctcgccaac aagtcctacg tgtcgatgag ccggatgaag aagctcgcgc ccgagatgca
     1861 gaagatcaag gagcgttacg gcgaggaccg tatgcgccag cagcaggcga tgatggagct
     1921 gtacaagaag gagaaggtga acccagcctc gggctgtttg ccgatcctag tgcagatccc
     1981 ggtcttcttc gcgctctata aggtgctgtt cgtcaccatc gagatgcggc atgcgccgtt
     2041 cttcggctgg atcaaggacc tgtcggcgcc tgacccgacg agcgtcttca atctgttcgg
     2101 ccttatcccc tgggatccgc ccacgttcct gatgatcggc gcctggccgc tcatcatggg
     2161 cttcacgatg tggttccaga tgaagctgaa cccggcgccg ccggacccga tgcagcagaa
     2221 gctcttcacc tggatgccgg tgatcttcac ttacctgctg gcctcgttcc ccgccggtct
     2281 cgtgatctac tgggcgtgga acaactccct ctccatcctc cagcagtcct tcatcatgtg
     2341 gcgccagggc gtggacattc cgctgctgga gaatctcggc atcaagccgt cccacggggc
     2401 ggcggacgag aacgaggaag ccaagtcggc caaaaccgag gcggcgcaaa agcgcagcgc
     2461 caaggccgcg cggcgggcga agcgggccgc gaagcaaacg aactccggcg cggccaagaa
     2521 ggcttcagcc aagaaggctt cagccaagaa ggcggaggac gctgagaccg atggcggcga
     2581 ggatcgcccg gcgtaagggc caattctcgt tcttgtcatc gccggcgaac gcgggcactc
     2641 catagcgatg gaccaccccg aattcaccgc cgacgagatc gcccggggcg aagagctgtt
     2701 caaggcgccg agcgaattcg tcaaaggcgt tgtcagcatc gctgcgcttc ccgacgaggg
     2761 cacgccggag atcgcctttg ccggacggtc caatgtgggc aagtcgagcc tgatcaatgc
     2821 gctcacgagc cggaagacgc tggcccgcgt ctcggtcacg ccggggcgga cgcgcgagct
     2881 caacttcttc acactcggcc ccgagagcga gctctatctg gtggacatgc cgggctacgg
     2941 ctatgccagg gccccaaaat cggaggtcaa aggctggacc cagttgatcc gggactatct
     3001 gagggggcgc cgccagctga agcgggtgtt cctgctgatc gatgcccgcc acggcatcaa
     3061 ggctaacgac cgggacacca tgaagctgct ggatgaggcc gccatgtcct atcaggcggt
     3121 actgaccaag gcggacaagc caaagcagag cgagcttcag gcggtgatcg ccaagacgga
     3181 ggacgagctg tcgaaacgcc cggcagccta cccccaaatc ctggtgacct cggcccgaac
     3241 cggctccggc attccggaat tgcgggccgc ggtggcaacc ttggcaccaa acggcgttgt
     3301 gccttgaggc caagcgccgg tataaggcgc aaatggccaa gaccaagaca gcccaggtaa
     3361 agtcgaaaga cccggcgcct cccaaggacg caggcccgct cgcggacgta ctcgcttccg
     3421 tccacgccaa cgccaagacc ttgtcggagg ccctgcccta tatgcagcgc tacgaccgca
     3481 agacggtggt ggtgaagtat ggcggccatg ccatgggcga tccggagctt gcccgcgact
     3541 tcgcccggga catcgtgctg ctcaagcagg ccggggtgaa cccggtcgtg gtccatggcg
     3601 gcggcccgca gatcggcaag ctgctggagc ggctcaacat caaatccgaa ttcaaggacg
     3661 gcttgcgggt caccgaccgg gagaccgtgg acgtggtgga gatggtgctc gccggctcgg
     3721 tcaacaaaga gatcgtctcg gccatcaaca accaaggcgg caaggccgtg ggcatttgcg
     3781 gcaaggacgc caatctgatg cgggccaagc ggctcgagcg gcgcgtgcgt gaccctcagt
     3841 caaatatcga gcaggtcctc gatctcggct tcgtcggcga gcccggcctg gttgaaccgc
     3901 atatcatgga cgtcattatc gagtcggacc tgatcccggt ggtggcgccg atcggcgtcg
     3961 gaccagacgg cgagacgctc aacatcaacg ccgacacttt tgcttcggcg ctggccgtgg
     4021 ccctgaaggc gaagcggctc ctactcctca ctgacgtgga cggcgtgctc gacaaagagg
     4081 gccgcttggt gcgctccatg tccacggcgg aagcgcaaag cctgatcgat gacggcacca
     4141 tcaacggcgg catgatcccg aagatcgagg gctgcgtgga cgtgatctcg gagggcgtcg
     4201 aggccgtggt catcatcaac ggcaaggtgc cacatgccgt gctgctggaa ctgttcaccg
     4261 agcacggcgt gggtacgctg atcgaacgca agcgccctgg cggcagccgc acgagggcgc
     4321 cgcgatgagc gctacccaag ggcaaggctt cggccatgta aagacgtggg tcttcgacct
     4381 cgacaacacg ctctacccgg ccgattgcga tctgttctcc cagatcgaca agcgcatggg
     4441 tgcgttcatc gccgatgcgt tcgatctctc catcgaagac gcacacgcct tgcggcagcg
     4501 ctactatttc gagcacggca ccacgctcgc cggtcttatc cggcttcacg gcgtgtgccc
     4561 ggactcgttt ctcgattatg tgcacgacat cgatcttgac gttgtcgccc cttcgcccga
     4621 gcttgtcgcc gccatcgacg cactgccggg ccggaagttc gtgttcacca acggctcgcg
     4681 caaacacgcc gaggccgtgg cggaaaagct cggcgtgctc gatcacttcg aggacatttt
     4741 cgacatccgc gcgctcgact ttgtgaaccc caagccgaca cgcgaagcct tcgaccggtt
     4801 caccggcacg cacggcatcg agaccgccaa cgcggcgatg ttcgacgacc tgccgcataa
     4861 tcttgagacc gcgcaagcga tcggcatgac cacggtctgg atcgattgcc gcgcgaccgg
     4921 ccggcccgag catcagagcc acgagacgtg gtccacactg cccgatcaca ttcatcatcg
     4981 taccgaggcg ctcgcgcctt tcctcaccgc catcgcgagc gctcttgctg aagacgccga
     5041 gagccccgcg acgcgcttgc cccaagcaca
//
LOCUS       DBNHVU010000099         5852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_782711, whole genome shotgun sequence.
ACCESSION   DBNHVU010000099 DBNHVU010000000
VERSION     DBNHVU010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_782711"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="AC16Y7_03810"
     CDS             <1..499
                     /locus_tag="AC16Y7_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HIR5708540.1"
                     /translation="SQETYDAIAPVMFQPKGETSLDALVATSISGPIGIPQEEAVYPQ
                     LVTTDGAPLNAQHDYVIRMSAEEMPPAKAFWSLTLYDLDEGFFIPNDRKKYSVGENGG
                     MKLNEDGGIEIHIAAEKPEGVPEENWLPIERKDLDLASQFRLYVPDLEKLKTWQAPKA
                     EKLSQ"
     gene            complement(809..885)
                     /locus_tag="AC16Y7_03815"
     tRNA            complement(809..885)
                     /locus_tag="AC16Y7_03815"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(849..851),aa:Arg,seq:acg)
     gene            1072..1440
                     /locus_tag="AC16Y7_03820"
     CDS             1072..1440
                     /locus_tag="AC16Y7_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708541.1"
                     /translation="MHRFLSKAVAILGAGFLAFAIAGCDAPSGGDSSASGAIGTYKTQ
                     DTQGNPMTITLAENGIASGDRAGESLDGSWKEEEGGAVVITWSDEWSTRIAKDGDKYT
                     KTAYKGGSMDGEPVSAEKTK"
     gene            complement(1460..2899)
                     /locus_tag="AC16Y7_03825"
     CDS             complement(1460..2899)
                     /locus_tag="AC16Y7_03825"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5708542.1"
                     /translation="MPAEKSMTRYVAGLSTKLLLLTIVFVMVAEVLVFVPSVSNFRRQ
                     WLMERLQAAQIASLAAEAAPGGQLPAALRDELLDSARVKAISVKKEDKRVLIVEMDMP
                     EEVDGAYDLRNAAWMNLIADALMVYVAPDGRIIRVVGEPGLNTGETIDVVMEEGPLKA
                     AMVQYGLDILGLSILISVITATLVFLALDALLVKPMTKLTRNMVRFAEQPEDPTRIIA
                     PSARRDEIGVAERELSTMQHELSQTLSQKTRLAALGLAVSKVSHDLRNMLSSAQLLSD
                     RLAEVKDPTVQRLVPKLIASLDRAIRLCVHTLDFGQAQETPPNRQRFELGQLVDEVGD
                     SLGLPRKDMIAWELDLEDRLEVDADRDQLYRVLSNLCRNAVQALESECRGVGAIMVTA
                     RREGAVALIEVADTGPGVPEKAKERLFQAFQSVARKGGSGLGLAIASELVQAHGGQIA
                     LVGNEGGATFRITIPDIVVELKQARRRRA"
     gene            complement(3012..4436)
                     /locus_tag="AC16Y7_03830"
     CDS             complement(3012..4436)
                     /locus_tag="AC16Y7_03830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase family protein"
                     /protein_id="HIR5708543.1"
                     /translation="MEEVLKPDLCVIGAGSGGLSVAAAAAQFGVPVVLIEKGLMGGDC
                     LNYGCVPSKALLAAGKRADAVRTASNFGIDVLEAQIDHRGVHQHVHGVIAAIAPNDSV
                     ERMTGLGVRVIQAQANFLDKFTVEAGRVLVKARRFVIATGSSPTVPAIPGLDEVSYLT
                     NETIFDIGERIQHLIILGGGPIGLELGQAHRMLGSHVTIFDTADFIAKEDPEAAKVLI
                     KRLEETEVEMYPNTRVERVEPSVQGLAVHFNKDGESVIIKGSHLLVATGRKPNLKQLT
                     LDRAGIAHSDAGVKVDEQLKTSNRRVYAIGDAIGGAQFTHLANYHASIVIKNALFRLK
                     PDVSAVNIPRVIYTDPELAQIGLTEQEAKQKYRRIRIYRWPYADNDRAQCEKQTDGFI
                     KVVTKKDGTILGVTIVGAEAGELLQLWIVAMARDIRIGDLISLVLPYPTLSELSKRVA
                     FAYHQPMLSKGWLRSIIGMLRHLG"
     gene            complement(4461..5252)
                     /locus_tag="AC16Y7_03835"
     CDS             complement(4461..5252)
                     /locus_tag="AC16Y7_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIR5708544.1"
                     /translation="MDTVSDMARGAADWLKRLWPLLLILGAGGFIFAMGWHRYLTLEH
                     LAGNRETLRAFIEGNLLLALAVFVALYALTVALSLPGGAVLTIAGGFLFGWLVGGAAS
                     VIGATLGAVAVFIVAKSALSDVLQARAGPMLSRFREGFHRDAFSYLLFLRLVPIFPFW
                     LVNLAPALLGVPFTTYVVTTILGIIPGTFAFSLAGAGLDSVIEAQQAAHQSCLAKAGE
                     GGQESCPYGLDPSALLTPELIAGLVALGVVALIPVVVKWFRRRAA"
     gene            5668..5802
                     /gene="rpmH"
                     /locus_tag="AC16Y7_03840"
     CDS             5668..5802
                     /gene="rpmH"
                     /locus_tag="AC16Y7_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR5708545.1"
                     /translation="MKRTYQPSRLVRKRRHGFRARMATKAGRKVIARRRAMGRKRLSA
                     "
BASE COUNT         1172 a         1834 c         1832 g         1014 t
ORIGIN      
        1 ctctcaggaa acctacgacg ctatcgcgcc agtaatgttc caacccaaag gcgagaccag
       61 tcttgacgcg ctcgtggcta cctcgatctc cggtccgatc ggcattccgc aagaggaggc
      121 agtctacccg cagctggtca ccaccgatgg cgctcccctg aacgcccagc acgactatgt
      181 catccgcatg tcggcggagg aaatgccgcc cgccaaggcg ttctggtcgc tgacgctcta
      241 cgacctggac gagggcttct tcatccccaa cgaccgcaag aagtacagcg tcggtgagaa
      301 tggtggcatg aagctcaacg aggacggcgg cattgagatc cacatcgccg ctgagaagcc
      361 ggagggcgtg cccgaggaga actggctgcc catcgagcgc aaggatctcg acctagcttc
      421 ccagttccgg ctctacgtgc cggacctgga gaaactgaag acctggcaag cgcccaaggc
      481 ggaaaagtta tcgcaataga cagcgccctc gatgctgcgc gacgccgcct ttcggcataa
      541 acaatgctga gccagtctcc agcattgccg tccagcacct tgtagccacc cggaacttgc
      601 tcaaccgtcc agggtgccgg aaaccggcgt gatgatgtca tgggagcctc cctcagttgg
      661 ctcccacaca cgaacggcac tttacagcaa tagaggttta cgatgctgac gccgctcggt
      721 cgcggcggtc tccacgggcc gtttacccca tggtttaccc cgcgaaatta actctcaagg
      781 gcgtcaaggt ctaactattt gaaattattg gcgcgccggg gaagattcga actcccgacc
      841 cccagattcg tagtctggtg ctctatccag ctgagctacc ggcgctaaaa gccgaaattc
      901 cgcgatcatg ttgagcggga ttggctgcct gcgaaacctg cccggctttt cgggggctcg
      961 cgttccgcct aggtaatacc ttgggacatc gaaggcaagg cttggaagtt tagccaaaat
     1021 ccatgccgat ttccgatatg gtgcgcccac caataataat ggagaagcac catgcaccgt
     1081 ttcctgagca aagccgtcgc catcctcggc gcaggcttcc tcgccttcgc catcgcgggc
     1141 tgcgacgcgc cctccggcgg cgacagcagc gccagtggcg ccatcggcac ctacaaaacc
     1201 caagacacgc aaggcaaccc aatgacgatc accctggccg agaacggcat cgcctccggc
     1261 gatcgtgccg gcgagagcct ggacgggtct tggaaggaag aagaaggcgg cgcggtcgtg
     1321 atcacgtggt ccgatgagtg gagcaccagg atcgccaagg acggcgataa gtacaccaag
     1381 accgcctaca agggcggctc gatggacggc gagccggtca gcgccgagaa gaccaagtag
     1441 cagacgctct tgaaaagact taagcgcggc gccgccttgc ctgcttcaac tcgacgacaa
     1501 tgtcgggaat ggtgatgcgg aaggtggcgc cgccttcatt gccgaccagc gcgatctgcc
     1561 cgccatgagc ctggaccaac tcggaggcaa tggcgagccc aagccccgag cctcccttgc
     1621 gcgccacgct ctgaaacgcc tgaaacaagc gctccttcgc cttttctgga acgccggggc
     1681 cggtgtcggc aacctcgatc aacgccacgg cgccttcgcg gcgagccgtg accatgatgg
     1741 cgccgacgcc gcggcattcg ctttcgagcg cctgcacggc gttgcggcag agattgctta
     1801 agacgcgata gagctggtcg cggtcggcgt cgacctccag gcgatcctcc aaatcgagtt
     1861 cccaggcaat catgtccttg cgcggcagtc cgagactgtc gcccacttcg tccaccaatt
     1921 gcccgagctc gaaacgctgg cggttcggcg gcgtctcttg cgcctgacca aagtccaggg
     1981 tgtggacgca gagccggatg gcgcggtcga gcgaggcgat cagcttcggc acaagacgct
     2041 gcacggtcgg gtccttgacc tcggccaggc ggtccgacag gagctgcgcg ctcgacagca
     2101 tgttgcgcaa gtcgtggctg actttgctca ccgcaagtcc aagcgcggcc agccgcgtct
     2161 tctgcgacaa tgtctgcgac agctcgtgct gcatggtgga aagctcgcgc tcggcgacac
     2221 caatctcgtc gcggcgggcg gagggcgcga tgatccgcgt cgggtcctcg ggctgctcgg
     2281 cgaagcggac catgttgcgc gtgagcttgg tcatgggctt caccaggagc gcgtcgagcg
     2341 caaggaagac gagcgtggcg gtgatgaccg agatgaggat cgacaatcca agaatgtcga
     2401 ggccgtattg caccatggcg gccttgaggg ggccttcctc catcaccacg tcgatcgtct
     2461 cgcccgtatt caagccaggt tcgccgacga cgcggatgat gcggccgtca ggcgcgacgt
     2521 agaccataag cgcatcggcg atcaggttca tccacgcggc gttgcgcagg tcataagccc
     2581 cgtcgacctc ctccggcatg tccatctcga cgatgagcac gcgcttgtct tcttttttca
     2641 ccgagatcgc cttgaccctg gcgctgtcga gcaactcgtc gcggagtgcg gcgggaagct
     2701 ggccgcccgg cgcggcctcg gcggcgagcg aggcgatttg cgccgcttgc agccgctcca
     2761 tcagccactg gcggcggaag ttggatacgg acggcacaaa gacgagcact tccgccacca
     2821 tgacgaaaac gatggtgagc agaagaagct tcgtcgaaag ccccgccaca tagcgggtca
     2881 tgcttttttc ggccggcatc tggcgcgacg cttgatcgtt caacggcaac gctcgcgaat
     2941 ggctttcggc ctcatgggcc gcgaaggtga attatagcgc aagttcaggg ggtttcccgc
     3001 gaaaactgcg attagcccaa atggcgcaac ataccgataa tgctgcgcaa ccagcctttg
     3061 gagagcatgg gttggtgata ggcgaacgcc acgcgtttgg agagctccga cagggtggga
     3121 tagggcagga ccagcgagat cagatcgccg atgcgaatgt ctctcgccat ggccacgatc
     3181 cacagctgga gcagttcgcc ggcctcggcg ccgacgatgg tgaccccgag aatggtgccg
     3241 tccttcttgg tgacaacctt aatgaagccg tcggtctgct tctcgcattg ggcgcggtcg
     3301 ttgtcggcat aaggccagcg gtagatgcgg atgcgccgat atttctgttt ggcctcctgc
     3361 tcggtgaggc cgatctgcgc cagttcggga tcagtgtaga tgacccgggg aatgttcacc
     3421 gcgctgacat ccggcttgag acggaacagc gcgttcttga tgacgatgct ggcgtgatag
     3481 ttcgccaggt gggtgaactg cgccccgcca atggcatcgc caatcgcata gacgcggcgg
     3541 ttggatgtct tgagctgctc gtcgaccttg acgccggcgt cgctgtgagc aatgccagcg
     3601 cggtcgagag tgagctgctt caagttgggc ttgcggcccg tggccacgag gagatgagag
     3661 cccttgatga tcacgctctc gccatccttg ttgaagtgca cggcgaggcc ttggaccgac
     3721 ggctcgacgc gctccacgcg cgtgttgggg tacatctcca cctcggtctc ctcgaggcgc
     3781 ttgataagga ccttggctgc ctccggatct tccttggcaa tgaagtccgc cgtgtcgaag
     3841 atggtgacgt gcgaaccgag catgcgatgc gcctgaccga gttcgagccc gatcgggccg
     3901 ccacccaaga tgatgaggtg ctgaatacgt tcgccgatat cgaagatggt ctcgttcgtg
     3961 aggtacgaca cctcgtcgag accgggaatg gccgggaccg tcggcgacga accggtggcg
     4021 atcacgaacc ggcgcgcttt gaccagcacg cggcccgcct ccaccgtgaa cttgtcgagg
     4081 aagtttgcct gcgcctgaat cacgcggacg ccaagcccgg tcatccgctc tacggagtcg
     4141 ttcggtgcga tcgcggcgat gacgccatga acgtgctgat ggacgccgcg gtgatcgatc
     4201 tgcgcttcga gaacatcgat accgaaattg gacgcggtgc gaacggcgtc ggcgcgctta
     4261 cccgccgcga gcagcgcctt ggacggcacg cagccatagt tgaggcaatc gccgcccatc
     4321 aggccttttt cgatcagcac gacgggaacc ccgaactggg ccgctgccgc cgcaaccgaa
     4381 aggccgcctg agcctgcgcc aatgacgcag agatcgggct taagcacctc ttccattcgc
     4441 ctaaatccct ctcaccacga ttaggccgcc cgccgcctga accacttcac caccaccgga
     4501 atgagcgcca cgaccccgag cgccaccaac ccggcaatca gctccggcgt cagtaacgcg
     4561 ctcggatcga gcccgtacgg acacgactct tgaccgccct ctcccgcctt tgcaaggcac
     4621 gattgatggg ccgcctgctg ggcctcgatg acgctatcga gtccggcgcc cgccagcgag
     4681 aaggcaaatg ttcccgggat aatgccaagg atggtggtaa cgacataggt cgtgaaggga
     4741 acgcccagca gggcgggcgc gaggttcacc agccagaacg gaaagatcgg cacgagccgc
     4801 aagaacagaa ggtagctgaa ggcgtcgcgg tggaagccct cgcggaaccg ggagagcatc
     4861 gggccggcgc gcgcctgaag cacgtccgac aaggcgctct tggcgacgat gaacacggcc
     4921 accgccccca gggtcgcccc aatcaccgaa gcggcgccgc cgaccagcca gccgaataga
     4981 aagccgccgg caatggtcag aaccgcgccg ccaggcagag agagtgcgac ggtgagggca
     5041 tagagggcca cgaagactgc gagcgccaag agcagattgc cctcgatgaa ggcgcgcaag
     5101 gtctcccggt taccggcgag gtgctccaaa gtcaggtagc ggtgccagcc catggcgaag
     5161 atgaagccgc ctgccccgag gatcagcaga agaggccata gccgcttgag ccaatccgcg
     5221 gcgccgcgcg ccatatccga cacggtatcc atgtttgagg gtactccttg gctgcctaca
     5281 gccaagagat aggctgaccc aacgggccgt gaaaacggtt tttcgcaccc ttgtgatggg
     5341 ccgtcactcg acgtgatggg acacggccct ggaagcgcga cttggcagac tggcatgctc
     5401 tggcggactg gcttggacga ggcggcctca gacaaggcgc gcgcgttgca gcgggaagcc
     5461 ctccttgatt aaccgacttt tgtgcaccgc cacaactgtt ggggccggac tttcccgctt
     5521 tccggccctt gggcgcgcga tttggcgcag aatcgcccgg atttgttgac ttgtaggcgg
     5581 acagaaccta taagccgccc cgaacctttt acgcctccca gacccgccca attcttaggg
     5641 gtgtggaggg acggcaggag aaagtccttg aaacgcacct atcaaccatc gcggctcgtc
     5701 cgcaagcggc gccacggatt tcgcgctcga atggcgacca aagcgggccg gaaggtgatc
     5761 gcacggcgca gagccatggg gcgcaagcgc ctgtcggcct aaggtgcggc ggatttccgc
     5821 ctcacccggt gacggccttc ggacgtctca ag
//
LOCUS       DBNHVU010000100         7448 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_186867, whole genome shotgun sequence.
ACCESSION   DBNHVU010000100 DBNHVU010000000
VERSION     DBNHVU010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7448)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7448)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7448
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_186867"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="AC16Y7_03845"
     CDS             complement(<1..776)
                     /locus_tag="AC16Y7_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohexomutase domain-containing protein"
                     /protein_id="HIR5708546.1"
                     /translation="MFPKPRQDLVPNTAEFERLPQVKATGFREYDARWLYPQEINLLG
                     MEAVGLGLTTLMRERGVPVRIVVGHDFRSYSSAIKAALITGLLAGGAEVHDIGLALSP
                     MAYFAQFDLDVEGVAMVTASHNDNGWTGIKMGMDRPLTFSPDDMTALKEIVMQAKYAP
                     YQDGARYIYLPDMAERYMADLLKHGKIKRRLKVVVSCGNGTAAAFAPHVLAELGCEVI
                     PLNCKLDYTFPAHNPNPEDLAMLDATRDAVRKHKADLGLV"
     gene            complement(850..1401)
                     /locus_tag="AC16Y7_03850"
     CDS             complement(850..1401)
                     /locus_tag="AC16Y7_03850"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HIR5708547.1"
                     /translation="MRLATKHLLQEGVLWTAGLLAVAIGVYFFDDLKSALGPQTERAP
                     AMATLTKAEQQQSGGFSGEVHLKGDGQGHFVFEGAVNDRPITFMADTGATIVALTYED
                     ARHVGLSPHSLNFSARVETANGIARVAPIMLDRVRVGDITVRDVRAAVADKGALTTNL
                     LGMTFLGRLKSFQVRGDELVLSQ"
     gene            complement(1442..2143)
                     /locus_tag="AC16Y7_03855"
     CDS             complement(1442..2143)
                     /locus_tag="AC16Y7_03855"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease family
                     protein"
                     /protein_id="HIR5708548.1"
                     /translation="MTTWLALFILALGAMMLVGNDSGMIAGLDATTFGYMAFLLALLV
                     YVAGGLMTRYAGGASAMVRDAVTWLALGLGLVTLYAYKDQLTPIAARVVGELLPGTAM
                     TVETSAGGLTEVKIRRRLDGHFTASVDVNGKTISMIVDTGATSIVLTPEDAKKAGFDP
                     DKLTYRIPVLTANGRTVAARVRLNEVAIGPLDRTKVEALIAQPGALTQSLLGMSFLSR
                     LRSYEFSGDFLTLRG"
     gene            complement(2140..2319)
                     /locus_tag="AC16Y7_03860"
     CDS             complement(2140..2319)
                     /locus_tag="AC16Y7_03860"
                     /inference="COORDINATES: protein motif:HMM:NF018630.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HIR5708549.1"
                     /translation="MNVCLLDEKLGLCVGCGRSGAEIAEWATMGADQRRAVMAILPDR
                     LKLLERLADAEGSST"
     gene            complement(2419..3972)
                     /locus_tag="AC16Y7_03865"
     CDS             complement(2419..3972)
                     /locus_tag="AC16Y7_03865"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5708550.1"
                     /translation="MSAGAAAEAPRVQAESANNHKRRAHRSVQEAREKLTYKSGHKPE
                     FDYELLMMFVKNELSAAVTTPLLAIIVAMGAMFWAPAQSLLLWLAAVFVAKGILIAIC
                     RQFVRAPRDDEATPGWRAKIAAAEVLYGVTWASVAFISPETQAEAAHTFIFASLLVVI
                     SMRMLFASTVTPVVYAGTLPMTIAIVIRFALLDNPFYWAMAAMAIGVHIYFVWLMNGF
                     SRTVMAMLEYRAEKDALIAELEQSKSISDDARMRAEEANLAKSRFLATMSHELRTPLN
                     AILGFSEIMRGEILGPHANPTYKEYAKDIHESGQHLLTLINEILDISRIEAGRYELNE
                     TPIKLIDIVEDCHRLMRVRAENKGLKIVEAFDDNMPRLWADERAVRQICLNLLSNAIK
                     FTPTGGTVTLKITRTKHGGQMLSVKDTGPGIPEDEIPRVLRSFGQGSLAHQTAEGGTG
                     LGLPITKGLTDLHDGNFELKSKLRYGTEVIVSFPRSRAMEALPRLTEPGEEPPEDPQV
                     PWRPPSSAA"
     gene            4222..4965
                     /locus_tag="AC16Y7_03870"
     CDS             4222..4965
                     /locus_tag="AC16Y7_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5708551.1"
                     /translation="MQISVIGCGDAFGAGGRLQTSFYVRSRSSRFLIDCGATTLVGMR
                     RLGIGLDDVDTVFVTHLHGDHFGGLPWLLIDAKYISNRTRPLVVAGPAGIEARFVALA
                     EALYPNSTRAEHNFDLVFVEYEERQPLEIGGITVTPFEVHHPSGAPPYALRFDADGRV
                     VAFTGDTGWVEALYDVARDADLFITECFQYDETLPIHLDYKTIDANYDKLGAKQILLT
                     HMGEGMLVELEKVDTSRYMISEDGMILDL"
     gene            4962..5585
                     /locus_tag="AC16Y7_03875"
     CDS             4962..5585
                     /locus_tag="AC16Y7_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HIR5708552.1"
                     /translation="MTAPVSALDTLLSAVRACRVCADAPQGKPLPHTPRPVLRASTTA
                     RLAVCGQAPGTRVHASGTPFSDPSGVRLRSWMGVTEDEFYDEARVAIVPMGFCFPGQD
                     AKGGDLPPRRECAALWHEKIFAALPQIELVLAVGAYAQAFHLGAAKGKTLQQTMERWR
                     ELLATTRDPKVLPLPHPSWRNNAWLKKNPWFEAELLPVLRREVRRLL"
     gene            5701..6180
                     /locus_tag="AC16Y7_03880"
     CDS             5701..6180
                     /locus_tag="AC16Y7_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR5708553.1"
                     /translation="MLDRMDRKILDILQKDCTLPVAEIGKQIGLSTTPCWRRIQKLEE
                     QGVIERRVALLDPKKVNVGVTVFVSITTSNHTKEWLQRFHAALEDFPEVVEFYRMSGQ
                     IDYLLRVVVPDIERYDAFYKRLIAKIDLSDVSSAFAMEQIKFTTALPLEYAAEERRA"
     gene            complement(6177..7049)
                     /locus_tag="AC16Y7_03885"
     CDS             complement(6177..7049)
                     /locus_tag="AC16Y7_03885"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5708554.1"
                     /translation="MSKLAWIGLGVMGYPIAGHIKDSGHDVTVFNRTCEKAERWVAEH
                     GGAFASTPAEAADGADFVFACVGNDNDLRDVTLGPDGVFQKLKPGAVFADHTTASAEI
                     ARELYAAAKDRNAGAIDAPVSGGQGGAENGTLTVMVGGDDADFAKAKPVIDTYARAVN
                     LIGPSGAGQLTKMVNQICIAGLIQGLAEALHFGRRAGLDMEKVIATISKGAAQSWQMD
                     NRWQTMLEGKFDHGFAVDWVRKDFGMCFDEARRNGVTLPVTALVDQFYADVQEMGGGR
                     WDTSSLIARLERDA"
     gene            complement(7153..>7448)
                     /locus_tag="AC16Y7_03890"
     CDS             complement(7153..>7448)
                     /locus_tag="AC16Y7_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HIR5708555.1"
                     /translation="EPHSDLGVKSFTMLLYLSDGEGHEELGTDIYDGTKEHVGRSPFA
                     PNAAMIFVPADDTFHGFEKRPIKGVRKSLIINYVTDEWRAREQLAFPEAPITA"
BASE COUNT         1373 a         2241 c         2361 g         1473 t
ORIGIN      
        1 aatacgagcc cgaggtctgc tttgtgcttg cgcacggcgt cgcgcgtggc gtcgagcatg
       61 gcgagatctt ccggattggg attgtgcgcc gggaaggtgt agtcgagctt gcaattgagc
      121 ggaatgactt cgcagccaag ctcggcgaga acgtgcggtg caaaggcggc ggccgtgcca
      181 ttgccgcagg atacgacgac cttcaggcgc cgcttaatct tgccgtgctt gagcaagtcc
      241 gccatgtagc gttcggccat gtcgggcagg tagatgtaac gcgcgccgtc ttgatagggc
      301 gcgtacttgg cctgcatcac gatctccttt agcgccgtca tgtcgtcggg agaaaacgtg
      361 agcgggcggt ccatgcccat cttgattccg gtccagccat tgtcgttgtg gctggcggtc
      421 accatggcga cgccttcgac gtcgagatcg aactgcgcga aataggccat gggtgagagc
      481 gctaggccga tatcgtggac ctcggcgcca ccggcgagaa ggccggtgat taacgccgcc
      541 ttgatcgccg atgagtagga gcggaagtcg tggccgacca cgatgcggac cggcacaccg
      601 cgctcgcgca tcagtgtggt cagcccaagc ccgaccgctt ccatgccaag cagattaatt
      661 tcttgcgggt agagccagcg cgcgtcgtat tcgcggaacc cagtcgcctt gacctgaggg
      721 agacgttcga actccgccgt gttcggaacg agatcttgcc gcggcttagg aaacattggc
      781 actccatgga tcgtcgtgag ggaggattcc ctctcgagtt atcatctttg agatgaaatg
      841 taagcgggcc tattgactaa ggacgagttc gtcgccgcga acctggaagc tcttgaggcg
      901 gcccaagaag gtcatgccca acaggttggt tgtaagagcg cccttatcgg ccaccgcggc
      961 gcgaacgtcc cgcactgtga tgtcgccgac gcgaacccgg tcgagcatga tcggcgcgac
     1021 gcgggctatg ccgttggcgg tctcgacgcg ggcgctgaaa ttcaggctgt ggggcgagag
     1081 gccgacatgc ctggcgtctt cataggtcag cgcgacgatg gtggcgccgg tatcggccat
     1141 gaaggtgatc gggcgatcgt tgaccgcgcc ttcgaacacg aaatggccct ggccatcgcc
     1201 tttgaggtga acctcgccag aaaacccacc tgattgctgc tgctcagcct tggtgagtgt
     1261 ggccatggct ggggcgcgct cggtctgcgg tccgagggcc gatttcagat cgtcgaagaa
     1321 gtagacgcca atcgccacgg ccaggaggcc ggcagtccat aggacgcctt cttgcagcag
     1381 atgtttcgtg gccagtcgca cggcgcttca ctcccgaacc cggcggtttt cgtggagttg
     1441 ctcaacctct cagggtcagg aaatcgccgg agaactcata ggagcggagg cggcttaaga
     1501 aactcatacc gagcaggctt tgggtcagag cgccgggctg ggcgatcagc gcctccacct
     1561 tggtgcggtc gagggggccg atggcgacct cgttcaggcg cacccgcgcg gccacggtgc
     1621 ggccattggc tgtcagaacg ggaatgcgat aggtgagctt atcggggtcg aaaccagcct
     1681 tcttggcatc ctcgggggtg agcacgatgg acgtggcgcc ggtgtcgacg atcatggaga
     1741 tcgtcttgcc gttcacgtcc accgaggcgg tgaagtgccc gtcgaggcgg cgcctgattt
     1801 tgacctcggt cagcccgccc gcgctcgtct cgaccgtcat agccgttccg ggcaggagct
     1861 cgcccacgac gcgtgcggca atgggtgtca actgatcctt gtaggcatag agcgtgacca
     1921 gcccgagccc gagcgccagc caggtcaccg catcgcggac catcgcgctc gccccgcccg
     1981 cgtagcgtgt catgaggccc ccggccacat agaccaggag cgcgaggagg aaagccatat
     2041 agccgaaggt cgtggcatcg agccccgcga tcatgccgct gtcgttgccc acgagcatca
     2101 tggcgccaag ggcaaggatg aacagggcga gccaagtggt catgtgctag agccttccgc
     2161 gtcggcgagg cgctccagga gcttaagccg atccggcaag atagccatga cggcccggcg
     2221 ctggtcggcg cccatggtcg cccattctgc aatctcggcg ccggaacgcc cgcagcccac
     2281 gcataggccc aacttctcgt ccagcaagca cacattcacg cacggcgttt ccaaggggtc
     2341 ccccaaggcc agatgtccat gcaatctatg taggcagtca ggcgagacag ggcaattgcc
     2401 cgcgcggcag ggcctagctc acgcagcgct cgatggcggg cgccagggca cttgtggatc
     2461 ttcgggcggt tcctcgccgg gctcggtgag gcggggcagg gcctccatgg cgcggcttcg
     2521 cgggaaggag acgatcactt cggtgccata gcggagcttg ctcttgagct cgaaattgcc
     2581 gtcatgtagg tcggtcagcc ccttggtgat aggaaggcca aggccggttc cgccttcggc
     2641 cgtctgatgg gccagcgatc cttgaccgaa gctccggagc acgcggggga tttcgtcttc
     2701 cgggatgccg ggacccgtat ccttcacgct caacatttgg ccgccatgct tcgtgcgcgt
     2761 gatctttagg gtgacggtcc cgccagttgg cgtgaacttg atggcgttcg acaagagatt
     2821 gaggcagatc tgccggacgg cgcgttcgtc ggcccagagc ctcggcatgt tgtcatcgaa
     2881 cgcttcgacg attttgagtc ccttgttctc cgcacgaacg cgcatcaagc ggtgacaatc
     2941 ctcaacgatg tcgatgagtt tgataggagt ctcgttcaac tcgtagcggc ccgcttcgat
     3001 gcggctgata tcgaggatct cattgatgag cgtaagcaga tgctggccgc tctcatgaat
     3061 gtccttggca tactctttgt aagtcgggtt ggcgtgcggg cccaaaatct cgccgcgcat
     3121 gatctctgaa aagccgagaa tggcgttgag cggcgtgcgc aattcgtggc tcatcgtggc
     3181 aaggaagcgc gacttggcga gattggcttc ttcggcgcgc atgcgcgcat cgtccgaaat
     3241 cgacttggac tgctccagct cggcgatcag ggcgtccttc tctgcgcggt attcgagcat
     3301 ggccatgacg gttctgctga acccgttcat gagccagacg aagtagatgt gcacgccgat
     3361 cgccatggcg gccatcgccc aatagaacgg attgtccagc agcgcgaagc gaatgacgat
     3421 ggcgatcgtc atgggcaagg tgccggcgta gacgacgggc gtcaccgtcg acgcgaacag
     3481 catgcgcatc gagatgacca cgaggagcga ggcgaagatg aaggtgtgcg ccgcctctgc
     3541 ttgggtctcg gggctgataa aggcgaccga tgcccaggtt acgccataga ggacttccgc
     3601 agccgcaatc ttggcgcgcc atccaggcgt cgcttcgtcg tcgcggggtg cccgcacgaa
     3661 ctgccggcaa atggcgatga gaatgccttt ggccacgaac acggcggcaa gccaaagcag
     3721 aagactttgt gccggcgccc agaacatggc gcccatggca acgatgattg ccagcagcgg
     3781 cgtcgtcacg gcggctgaca gctcgttctt cacgaacatc atcaggagct cgtaatcgaa
     3841 ctccggcttg tgtccggatt tgtatgtgag tttttcgcgg gcttcctgaa cgctgcgatg
     3901 agcgcgccgc ttatgattgt tggccgattc cgcttgaact cgcggggctt cggctgccgc
     3961 gcctgctgac atgactattt tgaacccgcg actgtctcac gaacaaaagc cgttgcggat
     4021 caatggcata gcgcacgcag gcgcggccga cgcctagacc cccactttag gcttaacgag
     4081 aactatctgc tcaaatccct aacagcgcgt ttactctcgc ttcggcctga cacggcgccc
     4141 ttgtcttcct cctaagaacc cccatttttc tagacggata gggctcgcga aagtgcgggg
     4201 agctggctga gaggggagcc catgcagata tccgtcattg gctgcggcga cgcattcggc
     4261 gctggtggcc gcctccaaac ttcgttctac gtccgctccc gctcgtcgcg gtttctgatc
     4321 gattgcggcg ctacgactct ggtgggcatg cgccggctcg ggatcggcct ggacgacgtc
     4381 gacaccgtct ttgtcacaca tctgcacgga gatcatttcg gtggtctccc gtggctcctg
     4441 atcgacgcga agtacatcag caaccggacg cggccgttgg ttgtcgccgg gcctgcggga
     4501 attgaagcgc gcttcgtcgc gctcgcggag gcgctctatc ccaactcgac aagggctgag
     4561 cacaacttcg atctggtctt cgtcgagtat gaggagcggc agccgctcga gatcggcggt
     4621 atcaccgtca ctccgttcga agttcatcat ccctcgggcg caccgcccta cgcgctgaga
     4681 tttgacgccg acggcagggt ggtcgcgttc acaggcgata cgggctgggt ggaggcgttg
     4741 tacgatgtcg cccgcgatgc cgatctgttc atcaccgagt gctttcagta cgacgagacg
     4801 cttcccatac atctcgacta caagaccatc gatgccaatt acgacaagct tggcgctaag
     4861 cagatcctgc tgacccacat gggcgagggg atgctcgtcg agttggagaa ggtcgatacg
     4921 tcccgctaca tgatctccga ggacggcatg atcctcgacc tatgaccgcg cctgtgtccg
     4981 cacttgatac gcttttgtcc gccgttcgag cttgccgcgt ttgcgccgac gcaccgcaag
     5041 gaaagcctct accgcacaca ccacgccctg tcttgcgggc gagtaccacg gcgcggcttg
     5101 ccgtttgcgg gcaagcgccg ggaacgcgcg tgcatgcttc cggcacgccc ttcagcgatc
     5161 cgtccggcgt tcgcttgcgg agttggatgg gcgtgacgga ggacgagttc tacgacgaag
     5221 cccgtgtcgc catcgtgccc atgggcttct gctttcccgg gcaagacgcg aagggtgggg
     5281 atctgccccc gagacgtgag tgtgccgcgc tatggcacga gaagatcttc gccgctctgc
     5341 cgcagatcga actcgtgctg gcggtgggcg cttacgcaca agcctttcat ctcggggcgg
     5401 cgaagggaaa gacactgcaa cagaccatgg agcgctggcg cgaacttctc gccaccacgc
     5461 gcgaccccaa ggtcttgccc ctgccgcacc cctcctggcg caataacgct tggctcaaga
     5521 agaacccctg gttcgaagcg gagctgctgc cggtcctaag gcgcgaggtc cgccggcttc
     5581 tctgagtgtc cttgcgggaa ggggaaaaat aatctattga tacgccctgc aggccgcgcg
     5641 tgatggaatt tcgttctcag gcctcgctgt ttctatcgat aattttatca ggcccaggca
     5701 atgctcgacc gcatggaccg caagatcctc gacatcttgc agaaggattg cacgctcccc
     5761 gtcgcggaga taggcaagca gatcgggcta tccaccacgc cctgctggcg gcgcatccag
     5821 aagctagagg agcaaggcgt gatcgaacgc cgcgtggcgc tgctcgatcc aaagaaggtc
     5881 aatgtcggcg tgacggtgtt cgtgtccatc accacgagca accacaccaa ggaatggctt
     5941 cagcggtttc acgcggcgct ggaggacttc ccggaggtgg tcgagtttta ccgcatgagc
     6001 ggccagatcg attatctgtt gcgcgtcgtg gtgcccgaca tcgaacgcta cgatgcgttc
     6061 tacaagcgcc tcatagcgaa gatcgatctc tccgacgtga gctcggcgtt cgcgatggag
     6121 cagatcaagt tcaccacggc gctgccgctc gagtacgccg ccgaagagcg tcgcgcttaa
     6181 gcgtcgcgtt ccaggcgcgc gatcaagctc gacgtgtccc agcgtccacc gcccatctcc
     6241 tgcacgtccg catagaactg atcgacgagc gccgtaacgg gaagggtgac gccgttgcgg
     6301 cgggcctcgt cgaagcacat gccgaaatcc ttgcgcaccc aatctacggc gaacccgtgg
     6361 tcgaacttgc cttcgagcat ggtctgccag cgattgtcca tctgccacga ttgagccgcg
     6421 cccttggaga tggtggcgat caccttttcc atgtcgaggc cggcgcgcct accgaaatgc
     6481 agcgcctcgg cgagaccttg gatcagtccg gcgatgcaga tctggttgac cattttggtg
     6541 agttggcccg cacccgatgg gccgatgaga ttgaccgcgc gcgcataagt atcgatcacg
     6601 ggcttcgctt tggcaaagtc ggcgtcgtcg ccgccgacca ttacagtcaa ggtgccgttc
     6661 tccgcgccgc cctgaccgcc tgagaccggc gcatcgatcg ctccggcgtt ccggtctttt
     6721 gcggccgcat acagttccct tgcgatttcc gcggaggccg tggtgtggtc ggcaaagacg
     6781 gcgcctggtt tgagcttttg gaacacaccg tcaggaccca gggtgacgtc gcggagatcg
     6841 ttgtcgttgc cgacgcaagc gaagacgaaa tcggcgccgt cggcagcttc ggccggcgtg
     6901 gaagcgaacg cgccgccatg ttcggcgacc caccgttctg ctttttcgca cgtccgattg
     6961 aagacggtca cgtcgtgtcc gctgtccttg atgtggccgg cgatcggata acccatcacc
     7021 cctagtccga tccatgcgag tttgctcatg cgtccgctcc gtccgtcctc gtttcggttt
     7081 cggcgtcacc ataaatgaaa tggccggggc gagcccggcc atttcgaagt gaattgtgaa
     7141 tgagacgtgc gatcaggcgg tgatcggcgc ctccggaaaa gcaagctgtt cgcgcgcacg
     7201 ccattcgtcg gtgacgtagt tgatgatcag cgatttgcgc acgcccttga tcggacgctt
     7261 ctcgaagccg tgaaacgtat cgtccgctgg tacgaagatc atcgcggcgt tgggcgcaaa
     7321 cggcgagcgg ccaacatgct ccttggtccc atcgtagatg tccgtgccga gctcttcgtg
     7381 gccctctccg tcggagaggt agaggagcat ggtgaaggac ttcacaccga gatcggagtg
     7441 aggctcga
//
LOCUS       DBNHVU010000101         5440 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_707452, whole genome shotgun sequence.
ACCESSION   DBNHVU010000101 DBNHVU010000000
VERSION     DBNHVU010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5440)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5440)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5440
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_707452"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="AC16Y7_03895"
     CDS             complement(<1..185)
                     /locus_tag="AC16Y7_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708556.1"
                     /translation="MAAQTNPPTAPDPDERVEVIMSDLPPQGTPAYRSLIRSAGDAKG
                     QMLPITKCEVWSVRRRHL"
     gene            complement(491..724)
                     /locus_tag="AC16Y7_03900"
                     /pseudo
     CDS             complement(491..724)
                     /locus_tag="AC16Y7_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010954007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1028..1243
                     /locus_tag="AC16Y7_03905"
     CDS             1028..1243
                     /locus_tag="AC16Y7_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708557.1"
                     /translation="MQPNEMPSLTSRPSSPISAVTHQLDLAFDHPRLNGLTSGDRQDV
                     IVIIAQLMLEACGKSLGVAEETGDEHA"
     gene            1230..3317
                     /locus_tag="AC16Y7_03910"
     CDS             1230..3317
                     /locus_tag="AC16Y7_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004675975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR5708558.1"
                     /translation="MSTLDLLPPAVLQRKAIVYVRQSTPHQVQMNLESQRRQYELVEV
                     ARQRGFRDVEVIDDDLGRTASGTVERPGFDRLVAALCAGKVGAVLCLDASRLARNGRD
                     WHHLLELCGLVEARVIDHDGIYDPCRPNDRLLLGMKGSISEFELGVIRARMHEAARSK
                     ARRGELRIPTPIGYTWDRSVGLTFDPDRRLQEVIRLVFAKFQELGSARQVLLWMASEN
                     VQFPYPTDGRTLTSFEWRPIRYRNIIGILKNPFYAGAYAYGKSEKRTTIVDGRARKSY
                     GWAKPMEAWEVLIKDHHEGYIDWSEFERNQAVLAGNAYSKAGDTKSGRGGGSLLAGLI
                     SCARCGRRLAVAYTGRYPRPIYRCHKANLQLGHRGCLGLAAKRIDAAIAAELLRAVAP
                     MAIEAAQEAERMMKEKDQDRRHIAELELRQAHYDASLAERRYAACDPDNRLIAAQLEK
                     AWEVTLQRVEACRQKLDGLQAPQIDAPRPDLTRLADGLSTVWTAPATTMRTRQRLARA
                     LITEILANIDEVKQEIVLVIHWKGGQHSELRFRKPKTGEHGRNTPEDAIAVMRSMAGR
                     WSDQDIAASLNRMGLPTGQGKTWTARRVASIRQVQGIHGYRSADKDGTWLTMSEAAAK
                     LGVSHHQMRRLIKEKVIVSEQVMADAPHQIRAADLETDQVEAALKRKGRPCRAIPENQ
                     LSMFPSTYEGGAE"
     gene            complement(3279..4673)
                     /gene="tnpC"
                     /locus_tag="AC16Y7_03915"
     CDS             complement(3279..4673)
                     /gene="tnpC"
                     /locus_tag="AC16Y7_03915"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HIR5708559.1"
                     /translation="MGTSDTSPASSNDEIASLRSLLAAKDARIAILEEKLRLAAVKQF
                     APKSEKLSALAQMDLFNEAEAFAAKPDAETQAVEITVPAYARARGKRKPIDASLPRVR
                     VEHDISEGKKTCACGCQLTRIGEVTSEQFDLIPARAQVLQHVRFKYACRSCEGTSHQS
                     PAVITAAMPAQPIPKSNASPGLLAHIATAKFADGMPLYRLEGILARDRIVVPRTTSAS
                     WMIRIGDMVVPLINLMHETQLSYDVLQMDETSVQVLKEDGRPATAKSYMWVRRGGPPG
                     QRIILFDYAPSRAGAIPMRLLDEYKGYLQSDAYSGYNAPAKREGVIHVGCLDHARRRF
                     VAAVKAQHGVAGTERGLAPEALLIIRKLYVIEKLARDARITADERHRLRNDKARPIWD
                     ELRAWLDANRNATPPLSLTGKAIGYLADEWPKLIRYLDDGRIEISNVLCENAIRPFVM
                     AVSFCTPFVSAWKH"
     gene            complement(4727..5143)
                     /gene="tnpB"
                     /locus_tag="AC16Y7_03920"
     CDS             complement(4727..5143)
                     /gene="tnpB"
                     /locus_tag="AC16Y7_03920"
                     /inference="COORDINATES: protein motif:HMM:NF017528.7"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HIR5708560.1"
                     /translation="MGLAAGRRGSAVSPPTIGDITIYLAQEPVDFRLGINGLSVLVEA
                     TLRFDPFSRNLFCFTNRRRNQIKVLYWQRSGFCLWQKRLEEEKFKWPAHLVPLASETT
                     RPPVVTLDEDQFLWLLDGLDLKHLKPHRALEYRSVL"
     gene            complement(5161..>5440)
                     /locus_tag="AC16Y7_03925"
     CDS             complement(5161..>5440)
                     /locus_tag="AC16Y7_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708561.1"
                     /translation="PLAPAGGEVGFADPRVVGRARACDDNLRAGARGRYASGARSFGH
                     HPDTIEIEVGGDCRIRVGSSFDGRALKVSARQYASDVDRRNRAAFAAA"
BASE COUNT          996 a         1704 c         1756 g          984 t
ORIGIN      
        1 aggtgccttc ggcgcaccga ccacacttcg cactttgtga tgggcagcat ctgccccttg
       61 gcgtcaccgg cggagcggat cagggacctg taggctggcg taccctgggg cggcaggtcg
      121 ctcatgatga cttcgacgcg ctcatcaggg tccggcgccg taggcgggtt cgtctgtgcc
      181 gccacagaag aggcgagtgc cgcaaggaga caaatacctt tgagaagcct gcttgccgac
      241 gcaggcaggg aaaaggcgat tccaactggc atgagcgctc cggaatttgt gaaacggggg
      301 gtctcgtcct gcagcaagtt gatcgtctgg cggagcaatg cctgcggagc accagatccg
      361 gatcgaaccg gctcggagcc gccccgcaga acattcggcc tattgcttcg ccggagccga
      421 cgaattcagt gtatccgccc cacgaacgac ctgggctcgt ggcttcgatt tccggttgac
      481 gggcgtcgtc tcaggcggcg acgctttgcg tcgccggtgt cgcgaccatc cagggcaaca
      541 gcgcctcgac atcggcgagc gtcgtcgcct gcggcagcag cgtgaacaca cgcttcaggt
      601 agtcgtagac gttgaggccg ttggccttcg ccgactcaac gaggctgtag agacgtgcgg
      661 aggcgtgggc gccggccggc gtgtcacaga agagccaggc ttttcgtccg acgacaattg
      721 ggcggtcatt gtgcccatat cgggcaggat gtgattgtcc actatcgctg gcatccgctc
      781 ttcggccagc gcgcgcgttg cattttgatg gagcagcgtg gctcgggcgc gatcgcacac
      841 atcgaactgg cgccgggcat agtcacgatg attgccgcgt ggaagctcga tccagtacat
      901 tgtgccggcc tcgaatttgg ttcacctcgc gcgtcattgc cggcgttggc cgctttggcc
      961 gagctgttgg cggccatcga atcgccgctc ccttccttgg atcgacaacg ccccgaggag
     1021 acgcgccatg caaccgaacg agatgccctc tctgacgtcg cgaccgagtt cacccataag
     1081 cgcggtcacg caccagctcg atttggcatt cgatcatccg cgcctgaacg gcctcacgtc
     1141 gggagatcgg caggacgtga tcgtgatcat cgcgcaactg atgctcgagg catgcggcaa
     1201 gagcctcggc gtagcagagg agaccggcga tgagcacgct tgatctcctc cccccggccg
     1261 tgctgcaacg caaggccatc gtttacgttc gccagtccac accccatcag gtgcagatga
     1321 acctggagag ccagcggcgt cagtacgagt tggtcgaggt tgctcgccag cgtggcttca
     1381 gggatgtcga ggtgatcgac gatgatctcg gccgcacggc gagcggcacc gttgagcgcc
     1441 ccggcttcga tcgattggtt gcggcgctgt gtgccggcaa ggtcggcgcc gtgctctgcc
     1501 tcgacgcttc ccgtctcgcg cgcaacggtc gtgactggca tcatctcctg gagctgtgcg
     1561 gtctggtcga ggcgcgtgtc atagatcacg atggcatcta tgacccgtgc cgaccgaatg
     1621 atcgcctact gctcggcatg aaggggtcca tctccgagtt cgagctgggc gtcattcggg
     1681 ccaggatgca tgaggcagca cgatcgaagg ccaggcgcgg tgagctgagg atcccgacgc
     1741 cgatcggcta cacttgggat cgaagcgtcg ggctgacctt cgatccggat cgacggctgc
     1801 aggaagtaat ccggctcgtc tttgccaagt tccaggagct gggcagtgcc cgtcaggtgt
     1861 tgttgtggat ggcgtcggag aatgtccagt ttccctatcc gacggacggg cgcacgctga
     1921 cgtcgttcga gtggcggccg atcagatacc gaaacatcat tgggatcttg aagaacccgt
     1981 tctatgcggg ggcctatgcc tacggtaaga gcgagaagcg cacgacgatc gtcgacggcc
     2041 gtgcgcgcaa gagctacggc tgggccaagc cgatggaggc ttgggaggtg ctcatcaagg
     2101 accaccatga gggctacatc gactggtccg agttcgagcg caatcaggcc gtgctcgccg
     2161 gcaatgccta cagcaaggcc ggtgacacga agtccggtcg tggcggcgga tcgcttctgg
     2221 ccggtctcat cagttgtgca cgctgcggcc ggcggctcgc ggtcgcctac accggccgat
     2281 atccgcggcc gatttatcgc tgccataagg ccaatctgca gctcgggcac cgcggttgtc
     2341 tcggattggc ggccaagcgc atcgatgccg cgattgccgc cgagctgctg cgcgcggtgg
     2401 cgccgatggc gatcgaggcg gcacaggagg cggaacgcat gatgaaggag aaagaccagg
     2461 accgccgtca catcgccgag ctggagctgc gccaggccca ctatgacgca tcattggcgg
     2521 aacgccgtta tgcagcatgc gatcctgaca atcgcttgat cgcagcacag ctggagaagg
     2581 cgtgggaggt cacgttgcag cgcgtcgaag cgtgccggca aaagctcgac ggcttgcagg
     2641 cgccccagat cgatgcgccc cgacccgacc tcacccggct agccgatggt ctctcgacag
     2701 tctggacggc acccgccacg acgatgcgaa cgcgccagcg tctagcgcgg gccctgatca
     2761 ccgagatcct cgccaacatc gacgaggtga agcaggagat cgttctggtg atccactgga
     2821 agggcggtca gcactccgag ttgcgcttca ggaagcccaa gaccggcgag cacggccgca
     2881 atacgcccga agacgcaatt gcagtcatgc gcagcatggc gggccgctgg tccgaccagg
     2941 acatagccgc ctcgctcaat cgcatggggc tgccgaccgg ccagggcaag acctggacgg
     3001 cacgccgtgt cgcctcgatc cgccaggtgc agggcatcca cggctaccgc tccgccgaca
     3061 aggacggcac atggctgacg atgagcgaag ctgctgcaaa gcttggtgtt tctcaccatc
     3121 agatgcgccg gctcatcaag gagaaggtca tcgtcagtga gcaggtcatg gccgatgcac
     3181 cgcaccagat ccgtgccgcc gatcttgaaa cggaccaggt cgaagccgcg ctgaagcgaa
     3241 aaggtcgccc gtgtcgggca attcccgaaa atcagctctc aatgtttcca agcacttacg
     3301 aagggggtgc agaatgaaac ggccatcaca aagggcctga tcgcgttctc gcagagcacg
     3361 ttcgatatct cgatgcggcc gtcgtcgagg tagcgaatga gctttggcca ttcgtcggcg
     3421 agatagccga tcgccttgcc cgtcagagat aacggcggtg tcgcgttgcg gttcgcatcg
     3481 agccatgcgc gcaactcgtc ccagatcggc cgcgccttgt cgttgcgcaa tcgatgccgc
     3541 tcatcggcgg tgatgcgggc atcgcgcgcc agcttttcga tgacgtaaag cttccgaatg
     3601 atcagcagag cttcgggcgc cagtccacgc tcggtgccgg cgaccccgtg ctgcgccttc
     3661 acggctgcga cgaacctccg ccgcgcatgg tcgagacagc cgacatgtat cacgccttcg
     3721 cgcttggccg gcgcgttgta tcccgaatag gcgtccgact gcagatagcc cttatactcg
     3781 tcgaggagcc gcatcgggat cgcgcctgcc cgcgacggcg cgtagtcgaa caggatgatg
     3841 cgctgtcccg gcggcccgcc tcgtcgaacc cacatgtagg acttcgccgt cgccggccga
     3901 ccatcctcct tcaacacctg tacgctggtc tcatccatct gcaggacgtc gtaggagagc
     3961 tgcgtctcgt gcatcaagtt gatcagcggc acgaccatat cgccgatccg gatcatccaa
     4021 ctcgccgagg tcgtgcgtgg caccacgatc cggtcccggg ccaggatgcc ctccagccga
     4081 tagagcggca tgccgtcagc aaacttcgcg gtggcgatgt gggcgagcag cccaggcgaa
     4141 gcgttggact tcgggatcgg ctgcgcgggc atggcagccg tgatgacggc cgggctctga
     4201 tggctcgtgc cctcgcacga ccggcaggca tacttgaacc gcacgtgctg aaggacctgc
     4261 gcgcgggccg gaatgagatc gaactgttcg ctcgtcacct cgccaatgcg cgtaagttgg
     4321 cagccgcagg cgcaggtctt ctttccctca gagatgtcgt gctcgacgcg cacgcgcggc
     4381 agcgaggcgt cgatcggctt ccgcttgcca cgggcgcgcg cgtaggccgg gaccgtgatc
     4441 tcgaccgcct gcgtctcggc gtcgggtttc gccgcgaacg cttccgcctc gttgaacaga
     4501 tccatctgcg ccagcgcgga gagcttctcc gacttcggag cgaattgctt caccgccgcc
     4561 agtctgagct tttcttcgag gatcgcgatg cgcgcatcct tggcggcgag cagggatcgc
     4621 agcgacgcga tctcgtcatt ggaagaggct ggcgatgtgt cagacgtgcc cacgatgaag
     4681 gcatatctga tttgtccgtg tcatggctgc atcgcggcga cgatcttcac agcacgctgc
     4741 ggtattcgag ggcacggtga ggtttgaggt gcttgagatc gagaccgtcg agcagccaca
     4801 ggaactgatc ctcgtcgagc gtcacgacag gtggacgcgt cgtctcgctc gcgagaggca
     4861 ccaagtgcgc tggccacttg aacttctctt cttccagccg cttctgccag aggcaaaacc
     4921 ctgagcgctg ccagtagaga accttgatct ggttgcgccg acggttggtg aagcagaaca
     4981 ggttgcggct gaacggatcg aaccggagcg tcgcctcgac caggacgctc aagccgttga
     5041 tgccgagccg gaagtccaca ggctcctgcg ccagatagat cgtaatgtcg ccaattgtcg
     5101 gcggcgatac cgccgatccc cgtcgccccg cagcaagccc catgaccgcc tcgtcgatgc
     5161 ctatgcagcc gcaaacgctg cacgatttcg acgatcgacg tcagaagcat attgtcgggc
     5221 gcttaccttc aaagcacggc cgtcgaagct cgatccgacg cggatgcggc aatcgccgcc
     5281 gacctcgatc tcgatggtgt ccggatggtg tccgaatgag cgggcgccgg acgcgtagcg
     5341 ccctctcgct ccggctctga ggttatcgtc acaggcacga gcccgcccga cgaccctggg
     5401 gtcggcgaac ccgacttctc cgccagccgg cgccagcgga
//
LOCUS       DBNHVU010000102        10740 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_411734, whole genome shotgun sequence.
ACCESSION   DBNHVU010000102 DBNHVU010000000
VERSION     DBNHVU010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 10740)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10740)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10740
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_411734"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="AC16Y7_03930"
     CDS             <1..596
                     /locus_tag="AC16Y7_03930"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR5708562.1"
                     /translation="ASGFNLTASVQRSDKHLVGVVLGGRTGAQRDAAMRTLFDKHWRK
                     ASTKKSKGGALIASLFGTSTPPPPSRKPAYALAGNTPSPAPATIEQGDVSGPGDTVRT
                     SLSPNRPAPRRATRDTYHVQVGAFTTSGDAQNRLGMVQQRASKLLNGHLPFTATFMKG
                     DTEWYRARFAGFSKNDAQSTCAALKRMKLECIALSAE"
     gene            complement(612..1331)
                     /locus_tag="AC16Y7_03935"
     CDS             complement(612..1331)
                     /locus_tag="AC16Y7_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HIR5708563.1"
                     /translation="MVYLLAGCALLIVLLLAARFLVRANPRSLAQNLRKVGGVALFAV
                     AGFLALRGALPIAIPIAAFALALLGRGGAGGLGGAFGSSQKSPGQTSSVGTDRLEMAL
                     DHDTGHMDGKCLVGRFAGRALSSLSDREAIELYQDFAADGQKEAALMEAYLDWRVADW
                     RDRAQDADTSTSGSRRAGTKGNRMSAEEARAVLEVGPNASEDDIRTAHRRLMKKMHPD
                     QGGSTYLAARINEAKEVLLGG"
     gene            complement(1344..2072)
                     /locus_tag="AC16Y7_03940"
     CDS             complement(1344..2072)
                     /locus_tag="AC16Y7_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR5708564.1"
                     /translation="MAEPKGSTKAPAKTARSDVERPRSSSTEVDAFLDQVHTLAPAAS
                     GRGRLLFAMDATMSRQPTWDLALSLQGDMFRAVKEVGGLDVQLLYFRGFNECQASRWV
                     GDPDALARLMRQVHCEGGHTQIRKVLSHARQENEKQKVHAVVYVGDCMEEDIDQLSQQ
                     AGELGLIGVPVFLFQEGDDGRAARAFKEIARLSRGAYCRFDAGSAGQLRELLTAVAVY
                     ATGGRKALAKSKSGAAVRLLEQLG"
     gene            2277..3128
                     /gene="panC"
                     /locus_tag="AC16Y7_03945"
     CDS             2277..3128
                     /gene="panC"
                     /locus_tag="AC16Y7_03945"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIR5708565.1"
                     /translation="MTLDIARTVRDLRTRTAAWRAAGESIALVPTMGAIHDGHLSLVR
                     LADGLADRVVTSLFVNPLQFGPREDFHAYPRDEARDADILAKAGSDLLYAPEASEMYP
                     DGFTTTVHIGDITEDLCGASRPNHFDGVATVVSKLLLQCAPDKAIFGEKDYQQLLVIR
                     RFVQDLDIPVDIIGGAIVREADGLALSSRNAYLSAGERKTAPILHETIQQTAADLAEG
                     RGGDDAAEAARFKLEAAGFRVDYVAIRDPETLKPLHGPVKSARVLAAVHLGKTRLIDN
                     VPVPPAG"
     gene            complement(3132..3575)
                     /locus_tag="AC16Y7_03950"
     CDS             complement(3132..3575)
                     /locus_tag="AC16Y7_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 family protein"
                     /protein_id="HIR5708566.1"
                     /translation="MTVHLVKLCVGIDSIQDLRDWQIKRLAALAQAGETPELCHRTMQ
                     TPRRRDDVLDGGSLYWVIKGAIIARQRIVDLKEATKDDGRRCCGIVLDPELVTTRPHP
                     RRAFQGWRYLEAADAPADTLALEAAGDDMPPGMREELRELRLIEW"
     gene            complement(3583..4419)
                     /locus_tag="AC16Y7_03955"
     CDS             complement(3583..4419)
                     /locus_tag="AC16Y7_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5708567.1"
                     /translation="MSFKDIIVYVDGTEASKARVAFAIALAKAQDADLIGVAFAPKAL
                     LPLSGADVGFADMSEVLASVKAQGEAALKGFKASAKEAGVAAEGRLMQGMSEEFPHDF
                     AAFARQVDLTILGQPRDGDPLIGQYALVERCLFASGRPVMIVPAAPAEIVPGGTVLAA
                     WDGSAEAARAFNDALIFLKAAKRVVLLIGVLDDTGEEHDPPTDDMMAHLKRHGVSVEV
                     ARVRAREGDIGRLLLAEAKDRGAEMIVMGAFHHSRWREFILGGVTLTMLEEATIPLLM
                     AH"
     gene            4633..4890
                     /gene="bufA2"
                     /locus_tag="AC16Y7_03960"
     CDS             4633..4890
                     /gene="bufA2"
                     /locus_tag="AC16Y7_03960"
                     /inference="COORDINATES: protein motif:HMM:NF050166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BufA2 family periplasmic bufferin-type
                     metallophore"
                     /protein_id="HIR5708568.1"
                     /translation="MKLNPKSGATIAMTAAAMLVSGTIATSALAAGGTTGKCFGVNAC
                     KGKSACKTAQSDCKGHNACKGKGWVSMTDTECAEKGGEFKS"
     gene            4970..5824
                     /gene="bufB"
                     /locus_tag="AC16Y7_03965"
     CDS             4970..5824
                     /gene="bufB"
                     /locus_tag="AC16Y7_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MNIO family bufferin maturase"
                     /protein_id="HIR5708569.1"
                     /translation="MSEHKPPYLGFGLGLRPQHYEEILNGDPDVDWFEVISENYMIRG
                     GQPLRVLDQIRERYPIVMHGVSLSIASTAPLDKDYLTGLKALADRVDPKWISDHLCWT
                     GVHGVNLHDLLPIPYTQEALTHVVDRVKQVQDFLGRRLTLENVSSYVTFGESEMSEWE
                     FVSEVAKRADCWLLFDVNNVYVSAYNHGFSTDDFLHGVPRDRIVQFHVAGHSHEDGHI
                     VDTHDHPVCDEVWDFYREAVTHFGPVSTMIERDDNIPPLAEVVAELDTARKIGREVQE
                     RAQRTAAE"
     gene            5821..6660
                     /locus_tag="AC16Y7_03970"
     CDS             5821..6660
                     /locus_tag="AC16Y7_03970"
                     /inference="COORDINATES: protein motif:HMM:NF021364.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HvfC/BufC N-terminal domain-containing protein"
                     /protein_id="HIR5708570.1"
                     /translation="MSSLKELQARFQAGILSGDDSVLADVKNSPSEDRTALFGVYRNA
                     YVLRLLEILGNDYELTHAYVGDATFAKLARGYVAAHPSDQRNARWFGRHLPAFAKDTP
                     PFSGHPEIAEIAALENALADAFDGPDADPLITEDLGAVSPDDWPSLIFTPHPTATQLR
                     FTTNAADIWLALKDETAPPDVVHLPEPQTVLVWRQEFTSRFRPLHAEEAMMWSEAIKG
                     TRFGVLCEMVATFAGEDEAGLRAATYLKAWIDAGLLKGAGSQPASSDEGAGNKALQRP
                     VSQ"
     gene            complement(6597..7418)
                     /locus_tag="AC16Y7_03975"
     CDS             complement(6597..7418)
                     /locus_tag="AC16Y7_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR5708571.1"
                     /translation="MFFYVAKAVWFVLQPSTLIALLIGYGAILIWTGWARWGRRFVTI
                     GAALLLIVGLSPLGNVLILPLEDRFPRADLVSPPAPKGIIILGGAENRLVGSARKAPT
                     LNEAGERLLEGAILAHRFPDAKVAFSGGDAGILYKSDSEADGAAAILTKLGVTPERLI
                     LEANARDTYENAVYLKKELTEDGNSDGRWLLITSAYHMPRSMGAFRQAGFDVEPWPVD
                     YRTRGKVDLTRPFDKVSEGLRRVDTATREWVGLLAYWLTGRCNALFPAPSSEDAG"
     gene            complement(7435..8814)
                     /gene="mgtE"
                     /locus_tag="AC16Y7_03980"
     CDS             complement(7435..8814)
                     /gene="mgtE"
                     /locus_tag="AC16Y7_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007197164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR5708572.1"
                     /translation="MQDAATEIQLRDEEGDIRSDFLHAVSHALETEDAARVRALTLDL
                     HEGDLADLIQVLRPDERAALIATLGDDFNVESLPELDEPVRDQVLEDMPPEQVAEALQ
                     QLESDEAVYLLEDLDRQEQEDILSKLPYFERIALQRSLEYPEDSAGRIMQTDLIAVPP
                     FWSVGQTIDYMRVADDLPDRFYEIFVVDPAYHLIGCVALNRILRSKRPTTIESITDVD
                     VHPVPVESDQEEVARQFERYNLTSAPVIDEDKRLVGVITADDVVEVVQEEASEDILAM
                     GGVGGESVSDTVWETTRLRFSWLLANLATAILASIVISFFEATIEQMVALAVLMPIVA
                     SMGGNAGTQTMTVAVRALATHDLGPANAFRVISREAAVGLLNGILFAVLMAAIAYFWF
                     GSGELGLVIGLAMIVNLFAAALAGILIPLGLEALDLDPAVASSVFVTTVTDVVGFFAF
                     LGLAALWLA"
     gene            9184..10368
                     /locus_tag="AC16Y7_03985"
     CDS             9184..10368
                     /locus_tag="AC16Y7_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5708573.1"
                     /translation="MARVFRPTLLEVFDLTNLELGTAYSVYGIVAMAAYFFGGPLADR
                     FPARALLTVALVSTAAGGLILMTVPPLSTLVMLYVYWGVTTIALFWAPLIRATRQWGA
                     DDAQGRAFGLLDGGRGMVAALMGLIMVMLFAWLLPANSETASLAQRTEALRQVILLLS
                     ATTCGAALLVWLTLPRRDLSQRGEKRKWSLRGVAHVFAMPTVWLQAAIILCAYVGFKA
                     IDDFSLYANQVVGLNEVDAARAGVVSLWVRPIAAIGAGFLADRIGAPTMTISSFAVLV
                     AGSIVLACGIIGAGMVWLFLLTIVCSSMGIFALRGLYFAIMREGKVSAAYTGSAVGLV
                     SVIGYAPDVFMGPLMGYLLDQSPGAPGHQHVFWVVTGFALLGLLASLLFAHVARAAKS
                     DA"
     gene            complement(10612..10731)
                     /locus_tag="AC16Y7_03990"
     CDS             complement(10612..10731)
                     /locus_tag="AC16Y7_03990"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708574.1"
                     /translation="MWTIDEADEMRRLIRLGVNGIMSARPSLLKQIAQEEGVW"
BASE COUNT         2022 a         3611 c         3214 g         1893 t
ORIGIN      
        1 gcgccagcgg gttcaacttg acggcctcgg ttcagcgtag cgacaagcat ctcgtcggcg
       61 tggtcctcgg cggccgtacg ggcgcgcagc gtgacgcagc tatgcgcacg ttgttcgaca
      121 aacattggcg gaaggcgtcg accaagaagt ccaagggcgg cgcgctgatt gcctcgctgt
      181 tcggcacgtc cacgccaccg ccaccgtcca gaaagccggc ctatgcattg gccggcaaca
      241 cgccctcacc cgcacctgcc acgatcgaac aaggtgacgt cagtggacct ggagacacgg
      301 tccggacgag cctttcgccg aaccgccccg cgcccagaag ggccacccgc gacacctacc
      361 atgtccaggt cggggcgttc acgacgtcgg gcgatgcaca aaaccggctc ggcatggtcc
      421 agcaacgggc ctcgaagctg ctcaacgggc accttccctt caccgccacc ttcatgaagg
      481 gggacactga atggtaccgc gcccgcttcg ccggcttttc aaagaacgat gcccagtcga
      541 cctgcgcggc cctgaagcgc atgaagctgg agtgcatcgc gctctccgcc gagtaacgcg
      601 ggctccgacc actagcctcc cagcaggact tccttcgcct cgttgatacg cgccgcaaga
      661 taggtggacc cgccctggtc gggatgcatc ttcttcatca gccgccggtg cgcggtgcgt
      721 atatcgtctt cgctggcgtt gggccctacc tccagcacgg cgcgcgcctc ctcggcgctc
      781 atgcggtttc ctttcgttcc cgcgcgccgc gaacccgatg ttgacgtgtc cgcatcctgc
      841 gcccggtcac gccagtcggc cacccgccaa tcgagatagg cctccatcag cgccgcctcc
      901 ttctgcccgt ccgcggcaaa gtcctggtag agctcgatcg cctcacggtc gctcaacgac
      961 gataaggcgc ggccagcaaa ccggccgaca aggcatttgc cgtccatgtg ccctgtgtcg
     1021 tgatccagcg ccatctccaa ccggtccgtt ccgacgctgg aggtctgccc gggcgacttc
     1081 tgtgaggacc cgaaggcgcc gcccagcccg ccggcaccgc ctcgcccaag aagcgcgagc
     1141 gcgaaggccg ctattggaat ggcgatggga agcgcgccgc gcagagccaa gaacccagcg
     1201 acggcgaaca aggcaacgcc gccgaccttg cgcaagtttt gggcaagcga gcgtggattg
     1261 gcgcggacaa gaaaccgcgc ggcaagcagc agaacaatga gaagcgcgca cccagcgaga
     1321 agatagacca tgccgaagaa ccgttatccg agctgctcga gcagccgcac cgccgcgccg
     1381 ctcttcgact tcgcaagcgc cttgcggccg cctgtcgcgt acacggcgac tgcggtcagc
     1441 agctcgcgga gctggcccgc cgacccggca tcgaaccggc aataggcgcc ccgcgacagc
     1501 cgcgcgatct ccttgaaggc tcgcgcggca cgcccatcgt cgccttcttg gaacaggaac
     1561 acgggcacac cgatgaggcc gagctcgccg gcttgctggc tcagctgatc gatatcttct
     1621 tccatgcaat cgcccacata gacgaccgca tgcacctttt gcttttcgtt ctcttggcgc
     1681 gcgtgggaca ggaccttgcg gatttgcgtg tgaccgcctt cgcaatggac ttggcgcatc
     1741 aaccgcgcca gggcgtccgg gtcgcccacc cagcggctgg cctggcactc gttgaagcct
     1801 cggaaataga gtagttggac gtccagccca cccacctcct tcacggcgcg gaacatgtcg
     1861 ccctgcaacg agagcgcaag atcccatgtg ggctgccggc tcatcgtcgc atccatggcg
     1921 aagagcaagc gccctcggcc cgaggccgca ggggccaggg tgtgcacttg atcgagaaag
     1981 gcgtcgacct cggtgcttga ggagcgcggc cgctccacgt cactccttgc ggtcttcgcg
     2041 ggggccttcg tggacccctt gggctctgcc atgttcacgc tccgcctgcg ttggaccggg
     2101 ctatgacatt caaaagccgc ggcaaatcgg aactcaccct atagatggcc cctcgctgcg
     2161 ccaacggcaa tgccgcccac gccggcgcgc actcaaaacg gttgggctcg cgctcaattt
     2221 agcccttccg gccgcatcct tcgagacggt atggtcgcgg ccctccggag accgccatga
     2281 cgctcgacat cgccagaacc gtccgcgatt tgcgcacccg caccgccgcc tggcgcgcgg
     2341 ccggggaaag cattgccctt gtccccacca tgggcgcgat ccacgacggc cacttgtctt
     2401 tggtccggct ggcggatgga cttgctgatc gcgtggtgac ctcgctcttc gtcaacccgc
     2461 tgcaattcgg gccccgcgaa gactttcatg cttacccgcg cgatgaagcc cgcgatgccg
     2521 acatcctcgc caaagccgga agcgatctcc tctatgcgcc ggaagcttcg gagatgtacc
     2581 cagacggatt caccaccacg gtacacatcg gcgacatcac cgaggatttg tgtggcgcga
     2641 gccgcccgaa ccatttcgac ggcgtcgcca cggtcgtcag caagcttctg ctgcaatgcg
     2701 cgccggacaa agccatcttt ggcgagaagg actaccagca gctcctggtc atccggcggt
     2761 tcgtccagga cctcgacatt cccgtcgaca tcatcggtgg cgctatcgtg cgcgaggccg
     2821 acggtctcgc gctgtcctcg cgcaacgcct atttgtcggc cggcgagcgc aagaccgcgc
     2881 cgattctgca cgagacaatc caacagaccg cagcggacct ggctgaaggc cggggcgggg
     2941 acgatgcggc cgaagcggca cggttcaaac tcgaagcggc cggctttcgg gtcgactacg
     3001 tggccatacg cgatcccgaa acgctgaagc cattgcacgg accggtgaaa agtgcccgcg
     3061 tgctggccgc ggtccatctg ggcaagacgc ggctcatcga taacgtcccg gtaccgccgg
     3121 ccggctagcg ctcaccactc gatcagacga agctcgcgca gctcctcgcg catgccagga
     3181 ggcatgtcgt cgccggcggc ctccagcgcg agcgtgtccg ccggcgcatc ggcagcttcc
     3241 agatagcgcc agccctgaaa ggcgcggcgc ggatgcggcc gcgtggtgac aagttccggg
     3301 tcgagcacga tcccgcaaca gcgccgtccg tcgtctttcg tggcctcctt aagatcgaca
     3361 atgcgctgcc gcgcgataat ggctcccttg atgacccaat agagcgaacc gccgtccaga
     3421 acgtcgtcgc ggcggcgcgg cgtctgcatg gtgcggtggc acagctccgg cgtctcgcct
     3481 gcttgcgcaa gcgcagccag ccgtttgatc tgccagtcgc gcagatcctg aatgctatcg
     3541 atcccgacac acagcttgac cagatggacc gtcatgacgg cctcaatggg ccatgagaag
     3601 cggaatggtc gcttcttcga gcatggtgag cgtgacgcca cccaagatga actcccgcca
     3661 gcgcgagtga tggaacgcgc ccatcacgat catttcggcg ccgcggtcct tcgcttctgc
     3721 aagcaggagc cggccgatat cgccctcgcg cgcgcggact cgcgccacct cgacactcac
     3781 gccatgccgc ttgagatggg ccatcatgtc gtcggtcggc gggtcatgct cctcacccgt
     3841 atcgtccaag acaccgatca acagcacgac gcgcttcgcg gcctttagaa aaatcagggc
     3901 atcgttgaag gcgcgcgcgg cctccgcgct tccgtcccaa gccgccagga cggtgccacc
     3961 gggcacgatc tcggccggtg ccgccggaac gatcatcact gggcgccctg aggcaaacag
     4021 gcagcgctcc accagcgcgt actgcccgat cagcggatcg ccgtcgcgcg gctggcccaa
     4081 aatcgtgagg tcgacctgac gggcgaacgc ggcaaagtcg tgcgggaact cctcgctcat
     4141 gccctgcatg agacggccct cggccgcaac gccggcctcc ttggcggacg ccttgaagcc
     4201 cttgagcgcg gcctcgcctt gcgccttcac gcttgcgagc acctcgctca tgtcggcgaa
     4261 cccgacatcg gcgccggaaa gcggcaggag cgccttgggc gcgaacgcca cgccgatgag
     4321 atcggcgtct tgcgctttgg cgagcgcgat ggcgaacgcg acccgcgcct tcgaggcctc
     4381 cgtgccatcc acgtagacga tgatgtcctt aaagctcatc cactccctcc gaacgcccaa
     4441 gatcatagcg cggcctccgg cccgccggta tcgccgatgg cactttatgc aagctcaagc
     4501 gttgttcacc gtggcgtgaa gagagtcaca gagctttgaa caggcgtgac aaaggcccga
     4561 ctggaaaagg gtctaggctc cggctctggc atttaacgcc acaattggga actcactcac
     4621 aggagagtac ttatgaagct gaacccgaag tctggcgcca cgatcgccat gaccgccgct
     4681 gccatgctcg tctccggcac gattgccacc tcggcgctgg ccgctggcgg caccacgggc
     4741 aagtgctttg gcgtgaacgc ctgtaagggc aagagcgcct gcaagactgc tcagagcgac
     4801 tgcaaaggcc ataatgcctg caagggcaaa ggctgggtct ccatgacgga caccgagtgc
     4861 gccgagaagg gtggcgagtt caagagctaa cccccaagac tgaaggagcc ggtcgcaact
     4921 cgcggccggc ttcctctctc ttgaatgtgg gctatgttgg tatccggtca tgagcgagca
     4981 caaacctcca tatctcggct tcgggctggg cctgcgcccc cagcactatg aggagatcct
     5041 gaacggcgac cccgatgtcg actggttcga ggtcatctcc gagaactaca tgatccgggg
     5101 cggccagccg cttcgtgttc tcgatcaaat ccgggaacgc tatcccatcg taatgcacgg
     5161 cgtgtcgctt tccatcgcct cgaccgcgcc gctcgacaaa gactatctca ccggcctcaa
     5221 agcgctcgcc gaccgcgtcg acccgaaatg gatttcggac catctctgct ggacgggcgt
     5281 gcacggcgtg aacctgcacg acttgctccc cattccctac acccaagaag cgctgaccca
     5341 cgtcgtggac cgcgtgaaac aagttcagga ctttctaggg cgccgcctca cgcttgagaa
     5401 tgtgtcgagc tacgtcacat tcggcgagtc ggagatgagc gaatgggaat tcgtcagtga
     5461 agtggcaaag cgcgccgatt gctggctgct cttcgacgtc aacaacgtct atgtcagcgc
     5521 ctacaatcac ggcttctcca cggacgattt tctacatggc gtgccgcgcg accgcatcgt
     5581 gcagtttcat gtcgccggac acagccatga ggacggtcat atcgtcgaca cccacgacca
     5641 tccggtctgt gacgaggtct gggacttcta ccgtgaagcc gtgacccatt tcggcccggt
     5701 gtcgaccatg atcgagcgtg acgacaacat cccgcccctg gccgaggtcg tggccgaact
     5761 cgacacagcc cgcaagatcg ggcgcgaggt tcaggagcgc gctcaacgca ccgcggccga
     5821 atgagcagcc tcaaagaact gcaagcacgc ttccaggccg gcattctgtc cggcgacgat
     5881 tcagtcctcg ccgacgtcaa gaacagccca agcgaggatc gcaccgcgct gttcggtgtc
     5941 taccgcaatg cctatgtgtt gcgcctgctc gaaatcctcg gcaacgacta cgaactcacc
     6001 cacgcctatg tcggcgatgc gacgttcgcc aaactcgcgc ggggctatgt ggccgcgcat
     6061 ccgtccgatc aaaggaatgc acgctggttc gggcgtcacc tacccgcgtt cgccaaggac
     6121 acgcctccgt tctccggaca tccggagatc gccgagatcg ccgcgttgga gaacgccttg
     6181 gcggatgcgt tcgacggacc cgacgccgac ccgctgatca ccgaagactt gggcgccgtc
     6241 tcacccgacg attggccgag cctcatcttc acgccgcacc caacggcgac acagcttcgc
     6301 ttcaccacca atgccgccga catttggctg gcgctgaagg acgagaccgc gccgccggac
     6361 gttgtgcacc tgcccgagcc gcagaccgtg cttgtctggc ggcaggaatt cacgtcccgc
     6421 ttcaggccac tccacgccga ggaggccatg atgtggagcg aagccatcaa aggcacgcga
     6481 tttggcgtct tgtgcgagat ggtggcgacc ttcgccggcg aggacgaggc gggcttacgc
     6541 gccgcaacct atctcaaggc ctggatcgat gccgggctgc tcaaaggcgc cggctctcag
     6601 cccgcgtcct cggacgaagg cgccgggaac aaggcgttgc agcggccggt cagccagtag
     6661 gccagcagcc cgacccattc gcgtgtcgcc gtatccacgc ggcgcaagcc ttcggagact
     6721 ttgtcgaacg gccgcgtcaa gtccaccttg ccccgcgtgc ggtaatccac cggccagggc
     6781 tcgacatcga agccggcctg ccggaacgct cccatggaac gtggcatgtg ataggccgag
     6841 gtaatcagca gccaacggcc atctgaattg ccgtcctcgg tcagctcctt cttgagataa
     6901 acggcgttct cgtaggtgtc tcgcgcattc gcctccaaga tcaaccgctc cggcgtcacg
     6961 ccgagcttgg tcagaatggc cgccgcgcca tccgcctcgc tgtcggactt gtagagaatg
     7021 cccgcgtccc cgccggagaa ggcgaccttc gcatcaggaa atctgtgcgc caggatcgcg
     7081 ccttccagca ggcgctcacc agcctcgttc aatgtcggcg ccttccgcgc actgcccacc
     7141 agccggttct cagcaccgcc taagatgatg atgcccttgg gcgccggcgg cgacacgaga
     7201 tcggcgcgcg ggaaccggtc ttccaacggc agaatgagca cgttgccgag cggcgacagg
     7261 ccgacaatga gcagcaaagc cgcaccgatg gtgacgaagc ggcgccccca ccgcgcccag
     7321 ccggtccaga tgagaatggc gccatagccg atcagcagcg cgatcagcgt cgatggctgc
     7381 agcacaaacc agaccgcctt ggcgacataa aagaacatcg gtccccctcg ccgctcaagc
     7441 taaccacaaa gccgctaggc cgaggaaggc gaagaagccc acaacatctg tgaccgtcgt
     7501 cacgaagacg gatgaggcaa cggccgggtc gagatccagc gcctccagac cgagcggaat
     7561 aaggataccc gccaaagccg ctgcgaacag gttgacgatc atcgccagcc cgatcacgag
     7621 ccccagctca ccactgccga accagaaata ggcaatcgct gccatgagga ccgcgaaaag
     7681 gatcccgttc agcaaaccaa cggcggcctc gcgtgagatc actctgaagg cgttggccgg
     7741 gccgagatca tgtgtggcca gcgcccgcac cgccaccgtc atggtttgcg tcccagcatt
     7801 gccccccatg gacgccacga tgggcatgag cactgccagc gccaccatct gctcgatggt
     7861 cgcctcgaaa aagctaatga cgatcgacgc caggatggcg gtggcaaggt tggccagaag
     7921 ccaactgaaa cggagccgcg tcgtctccca aaccgtgtcg ctcacggact ccccgccgac
     7981 gccacccatc gccaggatat cttcggaggc ctcttcttgc acaacctcga cgacgtcgtc
     8041 ggcggtaatc acacccacca agcgcttgtc ctcatcgatg accggcgccg aggtcaggtt
     8101 gtagcgctcg aactgacggg ccacctcctc ctgatcggac tccaccggga ccgggtgaac
     8161 gtcaacgtcc gtgatagatt cgatcgtggt cggccgcttg gagcgcaaga tccgattgag
     8221 cgcaacgcag ccaatgaggt gatacgccgg atcgaccacg aagatctcgt agaaccggtc
     8281 gggaagatcg tcggccacgc gcatatagtc gatcgtctgc cccaccgacc agaacggcgg
     8341 aacggcgatg agatcggtct gcatgatgcg gccggcggag tcttccggat attccaggct
     8401 ccgctgcaac gcgatcctct cgaaatacgg gagcttcgaa aggatgtcct cttgctcttg
     8461 ccggtcgagg tcctcgagca gatagaccgc ctcgtccgat tcaagctgct gcagcgcctc
     8521 ggcgacctgc tcgggtggca tgtcctcgag cacctggtcg cggaccggct cgtcgagctc
     8581 cggcagggac tccacgttga agtcgtcgcc caacgtcgcg atcaacgcgg cacgctcatc
     8641 aggacgaagc acctggatca agtcggccag atcgccctca tgcaggtcga gcgtcagcgc
     8701 gcgcacccgc gcggcatcct ccgtctccaa cgcgtgcgac accgcgtgga ggaagtcgga
     8761 acggatatcc ccttcctcgt cgcgcagttg aatttcggtt gcggcgtcct gcatcgcccg
     8821 tccaatcgct ggatttcaaa cgtcgagatg gtgggagcag gctccctaca ccgtgaccta
     8881 gaagacggcg actcgaccgt caccggcccg cctgtgatat caggccagcc atgccgtgcc
     8941 tatcgcggag gacggctaaa cccaccacgg cagcgatctg tccggtaagt ctggaagaaa
     9001 cacagcgcct cttctacatt gccagccacc tgaatttgga cggaacatct ctcgaagatt
     9061 ggaagctgcg gttggcgaca gcgacggcgg aactctcacc ctgatatgag cgatgcccga
     9121 caaggactga ccatcgcggc gctgatcatc gctggcgagt cggtatttct gctgcctttt
     9181 gttatggcac gcgtgttccg gccgacgctt cttgaagtgt ttgacctgac caatcttgag
     9241 ctgggcaccg cctattccgt ctacggcatc gttgcgatgg ctgcctactt ctttggcggc
     9301 cccctcgccg acagatttcc cgcacgcgcc ttgctgacgg tcgcgttggt gtctaccgcc
     9361 gctggcggac ttattttgat gacggtgccg ccactatcga cgctggtgat gctgtacgtc
     9421 tactggggcg tgaccacgat tgccctgttc tgggcgccac tgatccgcgc cacgcgccaa
     9481 tggggcgccg atgacgcgca aggccgcgct ttcggcttgc tcgatggcgg cagggggatg
     9541 gtggcggccc tgatgggctt gattatggtg atgctcttcg cctggctgct cccggcaaac
     9601 agtgaaactg caagtctcgc ccagcgcacc gaggcattga ggcaggttat cctgctgttg
     9661 tccgccacaa cctgcggcgc cgctttgctg gtctggctga cgctgccgcg tcgcgatttg
     9721 tcgcaaaggg gcgagaagcg gaaatggtct ttgcgcggtg tcgcgcacgt gttcgccatg
     9781 ccaaccgtct ggctacaggc agcaatcatt ctgtgcgcgt atgtcggctt caaagcgatc
     9841 gacgactttt cactctacgc aaaccaagtc gtcggactga acgaggtcga tgccgccaga
     9901 gccggcgtgg tttcgctttg ggtacgcccc attgccgcca ttggcgccgg cttcttggct
     9961 gaccggatcg gcgcgccgac catgacgatc agttctttcg cagtgctggt agctggtagc
    10021 atcgtgctgg cgtgcggcat cattggcgcg ggcatggttt ggctatttct gctgacgatc
    10081 gtctgcagca gcatgggcat ctttgcgctg agggggctct acttcgcgat catgcgggaa
    10141 ggcaaagtgt ccgccgccta cacaggctcc gccgtcggcc ttgtctccgt catcggctac
    10201 gcgccggacg tcttcatggg gcccctcatg ggttacctgc tcgaccagtc gccgggcgcc
    10261 cccggtcacc agcacgtgtt ctgggttgtc acgggatttg cgctgctcgg acttcttgcc
    10321 agtttgctgt ttgcccacgt cgccagagca gcaaaaagcg acgcgtaatg tccggaaacg
    10381 aagaatggtg cggtcgagaa ttgtgccact tactcgatag aacaacgact tgccccaagg
    10441 tgcgggcaca aaggcgcctt tggtgcacaa aaggaatttc cggcgacgcc cgcacccgaa
    10501 ccactattcc ctcggggcgc cctattgaga gtcgagatca aggggccccc taacctctga
    10561 gcctccatgg ctgtcccgca gatggtggtc agtaactatc tgctcaggca ctcaccacac
    10621 gccttcctcc tgagcgattt gtttgagcag gcttgggcga gcgctcataa tgccattgac
    10681 gccgaggcgg atcaggcggc gcatttcatc ggcctcgtca atcgtccaca catgtaccgc
//
LOCUS       DBNHVU010000103         4603 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1526212, whole genome shotgun sequence.
ACCESSION   DBNHVU010000103 DBNHVU010000000
VERSION     DBNHVU010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1526212"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..2163
                     /gene="recG"
                     /locus_tag="AC16Y7_03995"
     CDS             46..2163
                     /gene="recG"
                     /locus_tag="AC16Y7_03995"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR5708575.1"
                     /translation="MRPARLAPLFATVRALKGVGPRIEGLLNKLVGSRAETSHAHVID
                     LIWHLPVGLIDRARTPSIAEARVGELATLEVKVVEHRPGGGRRGRRAPYRILVEDDTS
                     ALELVYFRADAKYLERLLPIGETRIISGKLESYDGLLQMSHPDHVAAVDNAASLPLIE
                     PVYPLTAGISNTTMRKAVTAALTQLPELPEWIDAEVLAKQDWPDFADAIAALHAPQSE
                     ADFSPSVPARARLAYDELLANQLALAIIRQRLKKRAGRSLQAKGRLRQAIVDALPYTL
                     TGAQSRALADIDADMEDGNRMLRLLQGDVGSGKTIVALLAMANAVEAGTQSALMAPTE
                     LLARQHLATISAMAEAAGLRVAILTGRERGAARTAILDALQSGEIDILIGTHALFQEA
                     IQFRDLGLVIIDEQHRFGVHQRLALQGKGTGAGAELLVMTATPIPRTLLLTSYGDMDV
                     SQLNEKPPGRKPIATRTVPLERLDEVTDGAERAISDGAQVYWVCPLVEESDVLDVAAA
                     EERYASLRARFGDTVGLVHGRLTGKEKDAVMARFASGDLSILVSTTVIEVGVDVPNAT
                     IMIIEHAERFGLAQLHQLRGRVGRGAAESSCVLLYKQPLGEVARERLGVMRDTEDGFV
                     IAEEDLRLRGGGEVLGTRQSGLPAFRIATLPEHHELLAAARDAARLTLSRNPSLEGED
                     GARLRLLLYLFERDDAVRLMRAG"
     gene            complement(2182..2964)
                     /locus_tag="AC16Y7_04000"
     CDS             complement(2182..2964)
                     /locus_tag="AC16Y7_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HIR5708576.1"
                     /translation="MTTEKDNNTDEAPETEERPEDDLSQFGALEPTSSHFLARLRGYF
                     LTGLIIVGPVAITIYVVWWFINLVDGWVKPLIPEQYLPDNFLPFNVPGVGLIVGIFAL
                     MFVGALAANLFGRTIVSYGEMMLDRMPVVRGVYRLLKQIFTTVFSKAGTGFKRVGLIE
                     FPRRGLYAIVFVSGDPPAEVKGKLDNGELMTVFMPNAPNPTTGFVLFMPAKDVILLDM
                     AIDDAAKLVISAGLVAPPQAKLKELADAAKHKKPVPDEEPVV"
     gene            complement(3114..4304)
                     /locus_tag="AC16Y7_04005"
     CDS             complement(3114..4304)
                     /locus_tag="AC16Y7_04005"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR5708577.1"
                     /translation="MGAALLILGVLAAATPGAAQELGLKHGQKSLDSGNYEMAVRQFS
                     ATVNDKDSTAEQAAKALYLRGVALRRGGNPARAIADLGAAVWLGLSGSDKARALVNKG
                     LAYRAVGLSSQAENAIAQARRASGQANEILAQESRMTVASTSSSPIPDVAVGGSVWDR
                     IVPSMPSFGSSSSSSSQTASAPASTPAPQAAEAETRTAEAAPTTGWGAEVADESASQG
                     SSVSRWFGSLTGGNAPAPSSVPAAPTASRTTTAPPTTTASRTTAAPPTAESWAANTST
                     QKEGEGGTAVGRWFSRQTSSAPSAPAPAQPAAAPRGSGYTVQLANSGSPADAQALWKK
                     AKSSNPQLTAANPRIERVEIGSLGTFYTVKIGPYATSSEGTKVCNALKRRGTDCSVIS
                     PDGP"
     gene            complement(4421..>4603)
                     /locus_tag="AC16Y7_04010"
     CDS             complement(4421..>4603)
                     /locus_tag="AC16Y7_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR5708578.1"
                     /translation="ADPASTGCAVQTMLPVPTVHPFVTPLVYALPVQMIAYHTAVFMG
                     TDVDQPRNLAKSVTVE"
BASE COUNT          783 a         1489 c         1502 g          829 t
ORIGIN      
        1 tgcgatgtca agggtgcggc ccttttgctg ggttagactg cgatcatgcg ccccgcccgg
       61 ctcgctcctc tcttcgccac cgtacgcgcc ctgaagggcg tcggcccccg aatcgagggc
      121 ctgctcaaca agctcgtggg gtcccgagcc gagacttcgc acgcccacgt catcgatctg
      181 atttggcatt tgccggtcgg cctgatcgac cgcgcccgga cgccgtccat cgccgaggcc
      241 cgggtcgggg agttggccac gctcgaggtc aaggttgtcg agcaccggcc agggggcgga
      301 cggcgggggc ggcgcgcgcc ctaccggatc ttagtcgagg acgacacgag cgcgctcgag
      361 ctcgtctatt tcagggccga tgccaaatat ttggagcgcc tcctgccgat cggcgagacc
      421 cggatcatct ccggcaagct cgagtcttat gacggcctcc tgcagatgag ccatccggat
      481 catgtggctg ccgtggacaa cgcggcctcc ctgcccctca tcgagccggt ctatccgctg
      541 acggcaggca tctccaacac gaccatgcgc aaggccgtca ctgccgcgct gacccaactt
      601 cccgagctcc ccgaatggat cgacgccgaa gttctggcaa agcaggactg gccggatttt
      661 gctgatgcga ttgccgcgct ccatgcgccg caaagcgaag cggacttttc gccgtccgtg
      721 ccggctcgcg cgcgcctcgc ctatgacgag cttctcgcca accagctggc cctcgccatc
      781 atccgccaac gtttgaagaa gcgcgcaggg cgaagccttc aagccaaagg gcgtttgcgg
      841 caggccatcg tcgatgcact gccctacacg ctgacgggcg cgcaatcccg ggccctcgcg
      901 gacatcgacg ccgatatgga agacgggaac cgcatgctgc gtctcctgca gggcgatgtg
      961 ggcagcggca agaccatcgt cgcgctgctc gccatggcca acgctgtgga agccggcaca
     1021 cagtctgcgt tgatggcacc gaccgagctt ctggcgcgcc agcatctcgc caccatctcc
     1081 gccatggccg aggcggcggg cttgcgcgtt gcgattctga cggggcgcga acgcggcgcg
     1141 gcgcgcaccg cgatcctcga tgcgcttcag tccggcgaga tcgacattct catcggcacc
     1201 catgcgctgt tccaagaagc gattcagttt cgcgatctcg gacttgttat catcgacgaa
     1261 cagcatcgct tcggcgtgca tcagcggctc gcgctgcaag gcaagggtac gggcgccggc
     1321 gcggagcttc tggtgatgac cgcaacgccc atcccccgca cgctgctact gacgagctat
     1381 ggcgacatgg acgtatcgca gctcaacgag aagccgccgg gccgcaaacc catcgccaca
     1441 cgcaccgtgc cgctggagcg tctcgatgag gtgaccgatg gggccgagcg cgcgatttcg
     1501 gacggcgcac aagtctactg ggtctgtcca ctggtcgagg aatccgacgt actcgacgtc
     1561 gccgcggcgg aagagcgcta tgcaagcttg cgcgcccgct tcggggacac ggtcgggctc
     1621 gtccacggca gactaaccgg caaagaaaaa gacgcggtga tggcgcgatt tgcctccggc
     1681 gatctttcca ttctcgtgtc gaccactgtc atcgaagtgg gcgtggatgt gccgaacgcg
     1741 acgatcatga tcatcgaaca tgccgagcgc ttcggcttgg cgcagcttca ccaattgcgc
     1801 gggcgcgtgg ggcgcggggc ggcggagtcc tcttgtgtgc ttctctacaa gcagcccctt
     1861 ggcgaggttg cgcgcgagcg gttgggcgtg atgcgcgaca ccgaagacgg gttcgtcatc
     1921 gccgaggagg atttgcgcct acgtggcggc ggcgaggttc tcggcacacg gcagagtggc
     1981 ctgcccgcgt tccgcatcgc caccctgccc gagcatcatg aattgttggc ggccgcgcgg
     2041 gatgcggcgc ggctgacact ctcccgcaac ccgtctctcg aaggcgaaga cggcgcgcgg
     2101 ctgaggcttc tgctctatct gttcgagcga gacgacgccg tgaggctgat gcgtgcaggc
     2161 tagcgtcgcg gcgctttgcg tctaaacgac gggctcttcg tcagggaccg gcttcttgtg
     2221 cttggccgcg tcggcgagct ctttgagctt ggcttggggc ggcgccacga ggccggccga
     2281 aatcaccagc ttggccgcgt cgtcaatggc catgtcgagg aggatgacgt ccttcgccgg
     2341 catgaacagc acaaagcccg tggtcgggtt cggtgcgttc ggcatgaaca cggtcatgag
     2401 ttcgccgttg tcgagcttgc ctttcacctc ggccggcgga tcgccggaga cgaagacgat
     2461 tgcgtacagc ccgcggcggg gaaactcgat gagtcccacg cgcttgaacc ctgtgcccgc
     2521 tttggagaac acggtggtga agatctgctt caagagccga tagacgccgc gcaccaccgg
     2581 catgcgatcg agcatcatct cgccgtagct cacgatggta cgcccgaaca ggttcgcggc
     2641 gagtgcgccg acaaacatca gcgcaaagat gccgacgatc agaccgacgc ccggcacgtt
     2701 gaacggcaga aaattgtccg gcaggtattg ctcgggaata agcggcttca cccagccgtc
     2761 gaccaggttg atgaaccacc acaccacata gatggtgatg gccacggggc cgacgatgat
     2821 gagacccgtg aggaagtaac cccgcagccg cgccaggaag tgcgacgagg tcggctcgag
     2881 cgcgccaaac tggctgaggt cgtcctcggg acgctcctcg gtctcggggg cttcgtccgt
     2941 gttattatct ttctcggtcg tcattcgctc tcaactgtct cagggaaaca aagatgtgac
     3001 ggcaaggccg ttctcttatg cggccgttcc tatcacggca aatctacaga tatgaggcat
     3061 ggccgaatgc acgcgcgctg gcaagtccgc tttccgccgc tcattccgcc gccctatggc
     3121 ccgtccggcg aaatcaccga acagtcagtt ccccgccgtt tcagggcgtt gcaaaccttg
     3181 gtgccttcgg acgaggtcgc gtatgggccg atcttgacgg tgtagaacgt ccctaagctt
     3241 ccgatctcca ccctctcgat tcgcgggttt gcggcggtaa gctgcgggtt ggacgacttt
     3301 gccttcttcc acagcgcttg cgcatcggcc ggagatccgc tgttcgcgag ttgaaccgta
     3361 tagccgctgc cacgtggcgc ggccgccggc tgggccggtg ccggcgcgga aggcgccgaa
     3421 cttgtctggc gggagaacca gcggcccacg gccgtgccgc cctcaccctc tttttgcgtg
     3481 gaggtgttcg ccgcccagct ctcggcggtc ggcggagccg ccgtggtccg tgaggccgtc
     3541 gttgtcggtg gcgcggtggt ggtgcgcgag gccgttggcg ccgccggcac tgaggatggc
     3601 gcgggcgcgt tgccaccggt gagcgatccg aaccagcggc ttacggaact gccttgcgat
     3661 gccgattcat cggcaacttc cgcgccccag cctgtggtcg gggccgcttc ggcggtgcgg
     3721 gtctcggctt ccgccgcttg cggcgcgggc gtcgatgcag gggccgacgc ggtttggctg
     3781 ctcgacgaag acgacgagcc gaaactcggc atactcggca cgatcctgtc ccagacgctt
     3841 ccgcccacgg ccacatcggg tatgggcgag gaactcgtgc tggcgacggt catgcggctt
     3901 tcctgagcca atatttcatt ggcctgcccc gacgcccggc gcgcctgagc gatcgcattt
     3961 tccgcctggc tcgacaggcc gaccgcccga taggccagcc ctttattcac gagggcgcgg
     4021 gctttgtcgg agcccgacaa tccgagccat acggcggcgc cgagatcggc aatggcccgt
     4081 gcagggttgc cgccccggcg aagggcaacg cctctcagat agagcgcctt ggccgcctgt
     4141 tccgccgtcg aatccttgtc attcacggtg gcggagaact ggcgaacggc catctcgtaa
     4201 ttgccggagt cgagcgattt ctgcccgtgc ttgaggccca gctcttgagc ggcgcccggc
     4261 gtcgcggcgg ccagcacacc aaggatcagc agcgcggcgc ccatgcgacc acgccggaaa
     4321 aagcgccggt catgttcagg accgcgcggc cggaaagact tccgatgatc cgccatgtcc
     4381 ttgctcaagc aaataaatcg ggcaaatccg aggctgtaga ctattccaca gtaacagatt
     4441 tcgcgagatt ccggggctga tctacatccg tccccatgaa gacggccgtg tggtaggcga
     4501 tcatctgcac tggcaacgcg tagaccagcg gcgtcacgaa ggggtgtacg gtcggaacgg
     4561 gcagcatggt ctgaacggcg caaccggtgc tggcgggatc ggc
//
LOCUS       DBNHVU010000104         5497 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_560539, whole genome shotgun sequence.
ACCESSION   DBNHVU010000104 DBNHVU010000000
VERSION     DBNHVU010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_560539"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="AC16Y7_04015"
     CDS             complement(<1..221)
                     /locus_tag="AC16Y7_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708579.1"
                     /translation="MTDPIAEDEQAPPPLSREIRVIDIVDNAPQTIEGSAAEREAIKA
                     LLELVALGALRFDYTLRRGGGGRIHLSGRL"
     gene            complement(247..795)
                     /locus_tag="AC16Y7_04020"
     CDS             complement(247..795)
                     /locus_tag="AC16Y7_04020"
                     /inference="COORDINATES: protein motif:HMM:NF015914.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HIR5708580.1"
                     /translation="MALSLKPLAALLPWHRKVPQADQLYGAIVARARLPIFYQGFGVP
                     DNLEGRYVVLSLHLFTVLHRLKQAGTPEAARMAQDVADRFSADMETVLRELGVGDMAV
                     PKKMRKLMASGAGQLESFESALSEGAGALQSAIEATLPGEGEPSKAASAALTPYVLRV
                     VQHLDDKPIGGICAGKVSFPRI"
     gene            863..1372
                     /locus_tag="AC16Y7_04025"
     CDS             863..1372
                     /locus_tag="AC16Y7_04025"
                     /inference="COORDINATES: protein motif:HMM:NF016258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990063 - Bam protein complex [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HIR5708581.1"
                     /translation="MPKVKSASAWASLPLLAALLIGAAPIAGCGGQIDRHGHVFIDVD
                     LDQLQPGMSKDDVKTALGSPDTTSAIAGDAYYYISTTTKTVAFLKPKEIDRQVVAVYF
                     DGTERVRRVSNYGMKDGIVIDYHDDETPARGKDVDLVDQIFGNIASRGIAREQSKGKS
                     GGASGIPGI"
     gene            complement(1467..3590)
                     /locus_tag="AC16Y7_04030"
     CDS             complement(1467..3590)
                     /locus_tag="AC16Y7_04030"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIR5708582.1"
                     /translation="MTWVLYLIIACGALSIAYGVWTTKSVLAADPGTARMQEIASAIQ
                     EGAKAYLTRQYQAIALVGVAIFIALFFLLGLLPAIGFAVGAILSGAAGYIGMNVSVRA
                     NVRTAQASTKSLADGLSIAFRSGAITGMLVAGLALLGVSIYFLVLTKVLGYSPASRVV
                     IDALVALGFGASLISIFARLGGGIFTKGADVGGDLVGKVEAGIPEDDPRNPATIADNV
                     GDNVGDCAGMAADLFETYVVTIVATMVLASIYFAGGDRVMEMMIYPMAIGGACVITSI
                     IGTYFVRLGSSKSIMGALYKGVIATGVLSLILLYPVTDWLVGMDTVMRVGDVSFTGLS
                     LYFCGVVGLVVTALVVVITEYYTGTNYRPVQSIAKASVTGHGTNVIQGLAISLESTAL
                     PALIIIGAIIVAYGLAGLFGIAIAVTVMLALAGMIVALDAFGPVTDNAGGIAEMAGLP
                     EDTRKTTDALDAVGNTTKAVTKGYAIGSAGLGAVVLFAAYTEDLKYFAANADKFPYFT
                     GVDATFSLSNPYVVVGLFLGGLLPYLFGGIAMTAVGRAASAIVEEVRRQFKDKPGIMK
                     GEDRPDYGRAVDLLTKAAIKEMIVPSLLPVLSPVVFFFVIDWIAGKSAAFSALGAMLL
                     GVIVTGLFVAISMTAGGGAWDNAKKYIEDGNYGGKGSESHKAAVTGDTVGDPYKDTAG
                     PAVNPMIKITNIVALLLLAVLAHSG"
     gene            complement(3791..4810)
                     /gene="thiL"
                     /locus_tag="AC16Y7_04035"
     CDS             complement(3791..4810)
                     /gene="thiL"
                     /locus_tag="AC16Y7_04035"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HIR5708583.1"
                     /translation="MARKTKQGDPKGEGEFDIIARVFAPLAKDVGAFGLKDDAAILMV
                     TEGEELIVTCDTLVAGVHFLPDDPADSVGHKALAVNISDLTAKGARGYAYTLSLTLPP
                     DISIDWLEGFAAGLKVVQEQTDISLVGGDTTASPGPVTVTITALGQAAHQHAVMRLGA
                     MPGDRLYVSGTIGDAHLGLRLLRDPVLAKRWSLTENEAAYLVERYRRPGPNNALATLV
                     RNFARAAIDVSDGLVGDIEKLCAVSHVGVAIDAGNVPFSEAAKKALAFDPTLLPVLIT
                     AGDDYEVVLAVSEKSVAGFESESEASGARFTALGTLLAAKKGVSVHDEAGKTLKIKDK
                     GFKHF"
     gene            complement(4817..5299)
                     /gene="nusB"
                     /locus_tag="AC16Y7_04040"
     CDS             complement(4817..5299)
                     /gene="nusB"
                     /locus_tag="AC16Y7_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR5708584.1"
                     /translation="MAQGAKTSSRASLRSAARLAAVQALYQMDMTGIDLTDVLAEFEA
                     HRLGQELEGDQYHSAEAKFFRDLVEGVVREQRNLDPLIDQQLAEGWRLGRVDSILRAI
                     LRSAAFELTDRDDVPARVVISEYVDIAHAFFNEDEPKVVNGVLDQLAHKTRPKELTRQ
                     "
     gene            complement(5305..>5497)
                     /locus_tag="AC16Y7_04045"
     CDS             complement(5305..>5497)
                     /locus_tag="AC16Y7_04045"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR5708585.1"
                     /translation="AAGINQLALEHGVPIGSGIITCESSEQAWERASVDGRNKGGDAA
                     RACLTLMRLEQHFSLASRR"
BASE COUNT         1081 a         1738 c         1706 g          972 t
ORIGIN      
        1 agacgtcctg acaggtggat gcggccaccc ccgccacggc gcaatgtgta gtcgaagcga
       61 agcgcgccca aggcgaccaa ttcgagcagg gccttgatcg cctcacgctc ggccgcgctt
      121 ccctcgatcg tttgcggggc attgtccacg atgtcgatga cgcggatttc gcgcgacagt
      181 ggcggcgggg cttgctcgtc ttcggcgatg gggtcggtca tgctgttttc gcgccttctg
      241 tcagcctcag atcctcggaa agctgacctt gccggcgcag atgccgccaa taggtttgtc
      301 gtccaaatgc tggacaaccc ttagaacata gggcgtcagc gccgcgcttg cagccttcga
      361 gggctcaccc tcacccggca gcgtcgcctc aatggcgctt tgcagagcgc ccgcgccctc
      421 gctcaaggcg ctctcgaagc tttcgagctg gccggcgccg gaggccatca gctttctcat
      481 cttcttcggc accgccatgt ccccgacccc gagctcccgc agcacggtct ccatgtcggc
      541 cgagaagcgg tcggccacat cctgggccat gcgcgccgcc tcaggcgttc ccgcctgttt
      601 cagccggtgg agcaccgtga acagatgcag cgacagcacc acgtaccggc cctcgaggtt
      661 atcggggacc ccaaacccct gatagaaaat aggaagccgt gcccgcgcca caatcgcgcc
      721 atagagttgg tcagcttggg gtaccttgcg atgccacggc agcaaggcgg cgagaggctt
      781 caaagacagg gccatttcgt ctagctattt cacacttccg gcccgcttcg cgcgcgggtc
      841 tgaccgaggg gacgtccgaa tcttgccaaa ggtcaagtct gcatctgctt gggcttccct
      901 gcccttgctc gcggcgctgc tcatcggggc ggcgcccatt gccggctgcg gcggtcaaat
      961 cgaccggcat gggcacgtct ttatcgacgt ggatctcgac caactccagc cgggcatgtc
     1021 caaggacgac gtcaagacgg ccctcggctc gcccgatacg acgagcgcca tcgccggtga
     1081 cgcatattac tacatttcga cgaccactaa gacggtggcc ttcctgaagc ccaaagagat
     1141 cgatcgtcag gtcgtcgccg tctacttcga cggcacggag cgtgtccgtc gcgtgtccaa
     1201 ctacggcatg aaggacggca tcgtcatcga ctaccacgac gacgagaccc ctgcccgcgg
     1261 caaggacgtg gacttggtcg atcagatttt cggcaatatt gcaagccggg gcattgcgag
     1321 ggagcagagc aagggcaaga gcggcggcgc gtcgggtatt ccgggcatct agcggacaac
     1381 acgcccagag caagcagcgc gcctaaagaa aggccccgct tcgtttggag cgaggccttt
     1441 ttgtcttccg caatgtctga cggcgtttag ccgctgtgtg ccaatacggc gagcagcagc
     1501 agcgccacga tgttcgtgat cttgatcatt gggttcactg cggggcccgc cgtatccttg
     1561 taaggatcgc cgaccgtgtc gccggtcacc gcggccttgt gcgactctga gcccttgccg
     1621 ccgtagttcc cgtcctcgat gtacttcttg gcgttgtccc aggcaccgcc accagccgtc
     1681 atggagatgg cgacgaacag gcctgtcacg atgacgccga gcagcatggc gccgagcgcg
     1741 gagaacgccg cgctcttgcc ggcgatccag tcaatgacga agaagaacac gaccggtgac
     1801 agaaccggca gcagcgacgg aacgatcatc tccttgatcg ccgccttggt cagcagatcc
     1861 acggcacggc catagtcggg gcgatcctcg cccttcatga tgccgggctt gtccttgaac
     1921 tgacggcgca cctcctcaac gatggccgag gccgcacgac cgaccgccgt catggcgatg
     1981 ccgccgaaca gatacggcag cagaccgccg aggaagagac caacaaccac gtaagggttg
     2041 gacaggctga aggtcgcatc gacgccggtg aaatacggga acttgtccgc attggccgcg
     2101 aagtatttga gatcctcggt ataggccgcg aacagcacca ccgccccaag gccggccgag
     2161 ccaatggcat agcccttggt caccgccttg gtggtgttgc ccacggcgtc cagggcgtct
     2221 gtggtcttgc gggtgtcttc aggcagacca gccatttcgg caatgccgcc ggcattgtcg
     2281 gtgaccggac cgaacgcatc gagcgccacg atcataccgg ccagcgccag catgacggtc
     2341 acggcgatgg cgataccgaa cagtccggca agcccatagg cgacgatgat ggcaccgatg
     2401 atgatcaacg caggaagcgc cgtcgattcg agtgagatcg cgaggccctg aatgacgttc
     2461 gtcccgtggc cggtgacgga cgccttggcg atcgactgca ccgggcggta gttcgtgccc
     2521 gtgtagtact cggtgataac cacgaccagc gcggtcacta cgagcccaac gacgccacag
     2581 aagtagagtg acaggccggt aaagctcacg tcgccgaccc tcatgaccgt gtccatgccg
     2641 acaagccagt ctgtgacggg atagagcagg atgagagaca agacgccggt tgcgatcacg
     2701 cccttgtaca gcgcacccat gatcgacttg ctcgaaccga gacgtacgaa ataggtgccg
     2761 atgatcgagg tgatgacgca ggcgccgccg atggccatcg ggtagatcat catctccatg
     2821 acgcggtctc cgccggcgaa gtagatcgag gcgagcacca tggtggcgac gatggtcacc
     2881 acataggtct cgaacagatc ggccgccatg ccggcacagt cgccgacatt gtcgcccaca
     2941 ttgtccgcga tggtggcggg attgcgcgga tcgtcttcag gaataccggc ttccacttta
     3001 ccgaccagat cgccgcccac gtcggcgccc ttggtgaaga tgccgccgcc gagacgggcg
     3061 aagatcgaga tcagcgaagc gccgaagccg agcgcgacga gcgcatcgat gacgacgcgc
     3121 gaggccgggc tgtaaccaag caccttagtc agcacgagga agtagatgga aacaccgagc
     3181 agtgcgagcc ctgcgaccag catgccggta atggcgccgg agcggaacgc aatgctcagg
     3241 ccatcggcta gactcttcgt cgaggcctgc gcggtccgca cattagcccg aaccgagacg
     3301 ttcatgccga tatagccggc cgcgcccgac aaaatggcgc cgaccgcaaa accgatggcc
     3361 ggcagcaggc ccagcaagaa gaacagcgcg atgaaaatgg ccacgccgac gagagcgatg
     3421 gcctgatact gccgagtgag ataggccttt gcgccctctt gaatggcgga cgcaatttct
     3481 tgcatgcgcg ccgtacccgg gtcggccgcc aggacggatt tcgtcgtcca gacgccgtag
     3541 gcgatcgaaa gcgccccgca agcaatgatt agatagagaa cccaggtcat gacgaaccgt
     3601 ccccgtattt aagcttggtt tggaatcgaa ggttcacggc cgcaaaaagc aagacaccat
     3661 gggtatccgc gccggccccg ttgctccaga gaattggact taagccgcgc ggaaaatgcc
     3721 aaagttgggg gccggaagca acagaggggc gccggcgacg gcgatttcgc cgcagggctg
     3781 gagcggcggc ctagaagtgc ttgaatccct tgtcttttat cttgagggtt ttaccggcct
     3841 catcgtgcac gctgacaccc ttcttggcgg caaggagggt gccgagcgcg gtaaatcgcg
     3901 ccccgctggc ctctgattcc gattcgaagc ctgcgaccga tttctccgat acggcgagga
     3961 cgacctcgta atcgtcgcct gcggtgatga gaaccgggag caatgtgggg tcaaacgcaa
     4021 gcgccttctt cgccgcttcg gagaacggca cgttgcccgc atcgatagcc acgcccacat
     4081 gagagaccgc gcacagcttt tcgatgtccc cgacaaggcc gtcggagacg tcgatggcgg
     4141 cccgcgcgaa gtttcgcacc aacgtggcaa gcgcgttgtt cggtccaggg cgacggtagc
     4201 gctcgacaag atatgcggcc tcgttctccg tcaggctcca tctcttcgcc agaaccggat
     4261 cgcgcaagag gcgaaggccg agatgcgcat cgccgatcgt tccgctgaca tagagccggt
     4321 cgccgggcat ggcgccgagc cgcatcactg catgctgatg ggccgcctgg ccgagcgcgg
     4381 taatggtgac ggttaccggg ccaggtgaag cggtggtgtc gccgccgacc aggctgatat
     4441 ccgtttgctc ttggaccact ttaagcccgg ccgcaaaacc ttcgagccag tcgatcgaga
     4501 tatcgggcgg cagcgtgagc gacagcgtat acgcgtagcc ccgcgcgccc ttggccgtta
     4561 agtcagaaat attgacagca agcgccttgt ggccgacgga gtccgccgga tcgtcgggga
     4621 gaaagtgcac gcccgccacc agcgtgtcgc acgtgacgat gagttcctcg ccctcggtga
     4681 ccataaggat ggcagcatcg tccttcaggc cgaacgcccc cacgtccttg gcgagcggcg
     4741 caaagacgcg ggcgatgata tcgaactcac cctcgccttt cggatcacct tgctttgtct
     4801 tgcgggccat ggccgctcac tgtcgtgtta gttccttggg tcgcgtcttg tgcgcaagct
     4861 ggtctagaac gccgttcacc acctttggct catcttcgtt aaaaaaggcg tgggcgatgt
     4921 cgacatattc actgatgacc acacgagccg gtacgtcgtc gcggtcggtg agttcaaagg
     4981 cggcggagcg caggattgcc cgcaatatgg agtccacgcg gccgagccgc cagccttcgg
     5041 ccaactgttg atcgatcaag ggatcgagat tgcgctgctc acgcacgacg ccttcgacca
     5101 ggtcgcggaa gaacttggcc tcggcggaat gatattgatc gccctctagt tcctggccca
     5161 agcggtgcgc ctcgaactcg gcgaggacgt cggtcaagtc gatgcccgtc atgtccatct
     5221 gatacagcgc ctggaccgcg gcaagccggg cggcgctccg cagtgacgca cggctggatg
     5281 ttttggcacc ttgagccatg accgttaacg ccggctcgcc aaggagaaat gctgctccag
     5341 gcgcatcagg gtgaggcacg cgcgcgcggc gtctccaccc ttgttgcgcc cgtcgacgct
     5401 ggcccgttcc caggcctgct cgctgctctc gcacgtgatg atgcccgacc cgatcggcac
     5461 gccatgttcc aacgcaagct gattgatccc tgcggcg
//
LOCUS       DBNHVU010000105        22047 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_708370, whole genome shotgun sequence.
ACCESSION   DBNHVU010000105 DBNHVU010000000
VERSION     DBNHVU010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 22047)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 22047)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22047
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_708370"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="AC16Y7_04050"
     CDS             complement(<1..461)
                     /locus_tag="AC16Y7_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR5708586.1"
                     /translation="MATESITTKCAIAGGGPAGIMLGYLLARAGVDVVVLEKHEDFFR
                     DFRGDTIHPSTLQVMHELGVLDEFLTRPHQEVKTLGSEIGGEMVRIADFTHLPTVCKF
                     IVFMPQWHFLDFLALKAKQYPGFHLRMQAEVDELIKDGDRVAGLKAASPQG"
     gene            complement(542..1378)
                     /locus_tag="AC16Y7_04055"
     CDS             complement(542..1378)
                     /locus_tag="AC16Y7_04055"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HIR5708587.1"
                     /translation="MLSDEEIERYRRHLVLKQIGGEGQQRLKAARVLIVGAGGLGSPL
                     MLYLAAAGVGQIGLIDDDRVSVDNLQRQIAHNTESVDRLKTKSAAAMLERLNPHVETT
                     LFSTRLVAENALDIVRGFDIVADGSDNFATRYLVNDACYFAKAPLVTAAVGPFDGQLT
                     TFRAFEADENGNSRPSYRCLFPDPPPEGTVPACAEAGILGAVAGVVGCLQAVEVVKEL
                     LGIGEGLVGRLLLYDALSARFSTVAYGWDPENPLNGRNPRFRDLSHHRAGATAAAPAA
                     TP"
     gene            complement(1425..1874)
                     /gene="dut"
                     /locus_tag="AC16Y7_04060"
     CDS             complement(1425..1874)
                     /gene="dut"
                     /locus_tag="AC16Y7_04060"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR5708588.1"
                     /translation="MSATLRILRLPHAEELPLPAYQSAHAAGFDLLAAVPEDGPITLD
                     PSDRVLVPTGLVFELAAGYEAQIRPRSGLTLKHGIAVLNSPGTIDADYRGEVRVLLIN
                     LGNVPFLIRRGDRIAQAIVAPVTQVEIVEADALNETERGAGGFGSTG"
     gene            complement(1871..3094)
                     /gene="coaBC"
                     /locus_tag="AC16Y7_04065"
     CDS             complement(1871..3094)
                     /gene="coaBC"
                     /locus_tag="AC16Y7_04065"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR5708589.1"
                     /translation="MQGKRILLIVGGGIAAYKSLELVRRLTERGASVRVILTKGGAEF
                     VTPLSFSILSGDKTFTDLFDLKDEAEIGHIRLSREADLVVVSPATADILAKMATGLAN
                     DLASTVLLATDKPVLVAPAMNVRMWQHPATRRNVTTLRADGIHFVGPDDGDMACGEHG
                     PGRMAESEAIVAAIEGSLAAAPSGPLSGRHVLITSGPTHEPLDPVRYIANRSSGKQGH
                     ALARASVELGARVTLISGPVALPAPDGVTAIKVETAQEMLDAVIDALPADIAICAAAV
                     ADWRAQAEASQKLKKQDNVTELSLALAKNPDILATISVEGPMRPDLVIGFAAETQDVV
                     EHARDKRRAKGADWVVANDVSAGTGIMGGDRTEVHLVTGTSVEDWPAMSKDEMAAKLL
                     ARAGEALTTLSAAAE"
     gene            complement(3139..4716)
                     /gene="ubiB"
                     /locus_tag="AC16Y7_04070"
     CDS             complement(3139..4716)
                     /gene="ubiB"
                     /locus_tag="AC16Y7_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenylphenol 6-hydroxylase"
                     /protein_id="HIR5708590.1"
                     /translation="MNAIANMARLPKAGATLAWSGARVLPADTKLSGPLAVFGRVTAP
                     LRQNGAASGNEARLSTALTSLGPSYIKLGQFLATRDDIIGRELARDLSTLQDRLPPFS
                     HEEAVKAVETELEAPIGQLFAEFGPPVAAASIAQVHKAKVKAPDGTLSDVAVKVLRPG
                     VEQRFKKDLDSYFFAARLIERFHSPSRRLRPIAVVDTLSKSVAIEMDLRMEAAALSEM
                     AENSKDDEGFRVPKVDWTRSARRVLTLEWIDGVPIADHDKLRAAGHDLKPLGARLMQT
                     FLRHALRDGFFHADMHPGNLFVDGEGRIVAVDFGIMGRLGMKERRFLAEILYGFITRD
                     YMRVAQVHFDAGYVPRKHSVAQFAQALRAIGEPIMDRPASEISMAHLLGQLLQYTEVF
                     DMQTRPELIMLQKTMVVVEGVGRSLDPELNMWVVAEPVVKEWLESQLGAGARLEQAAE
                     GAASVGRFVGDLPRLLTQAERTVDAFGAIVGDGLRLDDQSVERLAEAQYTRDRWSRVG
                     IWVGAAALVAIALALLL"
     gene            complement(4732..5505)
                     /gene="ubiE"
                     /locus_tag="AC16Y7_04075"
     CDS             complement(4732..5505)
                     /gene="ubiE"
                     /locus_tag="AC16Y7_04075"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HIR5708591.1"
                     /translation="MPEDHKDTAPRDTEASFGFARVAEGARQDLVNQVFSKVAPRYDL
                     MNDLMSGGLHRLWKDELIGALAPPKGETPFALLDVAGGTGDVAFRFLDAAGRGGSAVI
                     CDISAEMMEAGKARAGGRRALEFVQANAENLPFEDRSFDAYTIAFGIRNVTHIDKVLC
                     EAFRVLKPGGRFLCLEFSSVEMPLLDTLYDAYSLNAIPQLGQLVAGDAESYRYLVESI
                     RRFPDQERFVQMIRDARFERVRYRNLTGGIAAIHAAWRL"
     gene            5593..6474
                     /gene="mutM"
                     /locus_tag="AC16Y7_04080"
     CDS             5593..6474
                     /gene="mutM"
                     /locus_tag="AC16Y7_04080"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HIR5708592.1"
                     /translation="MPELPEVETVRRGLAPILVGNSFARVEQRRPDLRFPLPERFSER
                     LQGRTIEALDRRAKYLLARLDDGEVLIMHLGMTGRFAIDKANGVGAPGRIARAPAQTP
                     KHEHIVFHLRDGSAVRYNDVRRFGIMDLVPSSSLDSHALFKSLGVEPLGDALTSEWLA
                     GKLKGKATSIKAALLDQRLIAGIGNIYACEALHRAAISPLKLAGALTTKTGKPTKKTD
                     ALVRAIKAVLESAIKAGGSSLRDYRHTDGRLGRFQHRFKVYGREGKPCPRTGCGGTVR
                     RNVQSGRSTFFCSTCQK"
     gene            6488..7306
                     /locus_tag="AC16Y7_04085"
     CDS             6488..7306
                     /locus_tag="AC16Y7_04085"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR5708593.1"
                     /translation="MLAEDIAQGEEAPAQNPYQTLIVTREGKVGLITLNRPKALNALN
                     TQLIGELNKALKAFDTDPEIGCIVITGNEKAFAAGVDIKEMHTKSFVEAYGSDFLAPF
                     DKINNIRKPIIAAVGGYALGGGCELAMCCDIILAADNAVFGQPEINLGIMPGAGGTQR
                     LTRAVGKAKAMELCLTGRQMNAEEAERASLVSRVVPAGELLGEAMKVAAKIANLSQHA
                     VMMIKEAINRGFATTLAEGTRFERGQFYSLFATEDQKEGMTAFIDKRKPAFKNR"
     gene            7369..7668
                     /locus_tag="AC16Y7_04090"
     CDS             7369..7668
                     /locus_tag="AC16Y7_04090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR5708594.1"
                     /translation="MRGKVTVIAFHRAKPGKERALRETLLALCGPTRVEKGCINYDLH
                     VSADDPSLLVFHENWVSKADLEAHLASPHIDAFREIAGELLAEPPDITLWSEAEA"
     gene            7814..8080
                     /gene="rpsT"
                     /locus_tag="AC16Y7_04095"
     CDS             7814..8080
                     /gene="rpsT"
                     /locus_tag="AC16Y7_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR5708595.1"
                     /translation="MANTKSAKKAVRQIARRTEANKARRSHMRSSIRRVEEAIATGSK
                     EAAQAALKEAEPIMARTAQKGLLHRRAASRKVSRLAKRVSAMGA"
     gene            8671..9117
                     /locus_tag="AC16Y7_04100"
     CDS             8671..9117
                     /locus_tag="AC16Y7_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR5708596.1"
                     /translation="MGAQSDENPVDEVDVATAWNGLKATPRSQLVDVRTRAEWTYVGI
                     PDLVPLGKRTVLVEWQTFPDQSVDPRFAERLANELTALGVQLDDDLFFICRSGSRSLA
                     AAKAMAAAGYRACHNVSEGFEGPLDDARHRGAQLGWKAIGLPWQQS"
     gene            9232..10683
                     /gene="dnaA"
                     /locus_tag="AC16Y7_04105"
     CDS             9232..10683
                     /gene="dnaA"
                     /locus_tag="AC16Y7_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR5708597.1"
                     /translation="MAEAQARAHHEPEEQCAPSEAWTRVKARLRGEVGEDVYASWFKG
                     VEVQEVGEGVVHLTVATRFLRNWLRTHYYESVLRHSRGEWPAAERLEFTVRQPHFAVE
                     PVKDPPRVQRAPAPAANDRTLLPMPLRTGYEGFEGSPLDPRLSFSSFVVGNTNKLAHA
                     AAQQVAHASSSPQSSFNPLFLHGNVGLGKTHLLHAIAWEVTQQKPEAEVLYLTAERFM
                     SGFVQALRARDALAFKEKLRNIDILLIDDMEFLQGPTIQQEFCHTLNSLIDGGRQVVV
                     AADRAPTHLDKLDMRMRSRLGGGLVAEIKAMDYDLRHKILEKRAQEACTETNGLEVSD
                     TVLAFLAERLSESGRELEGAVHRLRASYQLTDEPVTIAIAEQIVRDLMRGAEPRRVRI
                     DDILRTVSKHYGVNRGDLLSGRRNRSIVRPRQIGMYLAKLLTSRSLPEIGRRFGNRDH
                     TTVLHAIRKIEQLMSDDNQLREEIELLKRLLRD"
     gene            10887..12008
                     /gene="dnaN"
                     /locus_tag="AC16Y7_04110"
     CDS             10887..12008
                     /gene="dnaN"
                     /locus_tag="AC16Y7_04110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR5708598.1"
                     /translation="MRLTIDKPGLAKSLAHVASVVERRNTIPILSNVLLTADANELKL
                     TATDLDMELVERVACTAQGDGAVTVPAHMFYDIVRKLPDGEIELNRDAEQERLVITSG
                     PAQFSLQTLPADDFPSLSVEDLDHAFKVPAGDLKRLIEKTRFAISTEETRYYLNGIYL
                     HEAKGESEGKPTLRAVATDGHRLAQVELPLPSGAAGMPGVIVPRKTVQELSKIVEDED
                     GEVTIELSPSKIRIAAGRVVLTSKLIDGTFPDYERVIPQGNDKQLEVDNETFSKAVDR
                     VSTLSSDKGRAVKLALADGTLTLSVNNPESGSATEKLSVEYEHDPLEIGFNARYLLDI
                     SDQLEGETAEFHLADPGSPTMVCDVKDPSALYVLMPMRV"
     gene            12005..13198
                     /gene="recF"
                     /locus_tag="AC16Y7_04115"
     CDS             12005..13198
                     /gene="recF"
                     /locus_tag="AC16Y7_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579351.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR5708599.1"
                     /translation="MNATPRQHTPEDAHASQPDLTRLAVRTLKLTNFRNYASARLDLD
                     ARPVVLTGDNGSGKTNLLEAVSLLGPGHGLRGRPYVELARKDGDGGFAIAATVMTRAG
                     ETEIGTGLLASAREEGTGRTVRINGKDHSAGALGEYVKQVFLIPAMDGLFTSGGSERR
                     RFLDRLVLSIDPLMRTPLNRYERAMRQRNRLFQMREHSRSLFEGLEEQMAEAGVAIAA
                     ARYDAVERLTGLIEETREAQGEGPFPHAVLALHGTLESELTERPATEVEDVFRILLED
                     GRERDRAASRTLEGPHRSDLLVVHGPKNAPAAQCSSGEQKALLLGLILAKAELIKSLE
                     GNAPLILLDEVAAHLDAARREALFGEILRLGVQAWMTGTDKELFSPIAQDAQIFAVNQ
                     GNIEA"
     gene            13266..15698
                     /gene="gyrB"
                     /locus_tag="AC16Y7_04120"
     CDS             13266..15698
                     /gene="gyrB"
                     /locus_tag="AC16Y7_04120"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018327165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HIR5708600.1"
                     /translation="MTPAAKRKAEAAADYGAESIKVLKGLDAVRKRPGMYIGDTDDGS
                     GLHHMVYEVVDNAIDESLAGYCDEINVMLNADGSVTVEDNGRGIPTEIHKGEGVSAAE
                     VIMTQLHAGGKFDQNSYKVSGGLHGVGVSVVNALSSMLELTIWRDGKEHTMRFADGVP
                     EVPLKPMRDSQGRTGTRITFVPSTNTFTMVDFDYATLEHRLRELAFLNSGVRIILSDL
                     RGVEPKTEEMHYEGGLRAFVQFLDRTKHPLINEPVVVEKMQSRITVEAALWWNDSYHE
                     NVLCFTNNIPQRDGGTHLAGFRAALTRTVNTYAAASGIAKKEKVALTGDDAREGLTCV
                     LSVKVPDPKFSSQTKDKLVSSEVRPVVESVVAERLSQWFEENPGEARNIVQKIVEAAA
                     AREAARKARELTRRKGALDVASLPGKLADCQERDPAKSEIFLVEGDSAGGSAKQARER
                     AFQAVLPLRGKILNVERARIDKMLSSAEIGTLITALGTSIGDEFDLAKLRYHKIIIMT
                     DADVDGSHIRTLLLTFFYRQMPSIIEAGHLYIAQPPLYKVKRGNHERYLKNEDGLEDY
                     LIGEGLQDTVLTTGEDAARGGGDLRDIVEQARLVTGILRGLHSRYPRFVIEQAAIAGA
                     LSPDVLKDTKQAQAAAAYIAKRLDSLSEETERGWHGEALPEGGLRFWRELRGVMESHL
                     IDGPFIASADALKLDQYAPHLQEVYAKPATLKRKDTSLEVHGPSELLDAVMETGRKGL
                     TLQRYKGLGEMNADQLFETTLDVDTRTILQVKIREGEDADDIFSRLMGDVVEPRREFI
                     QENALEVANLDV"
     gene            16065..17903
                     /locus_tag="AC16Y7_04125"
     CDS             16065..17903
                     /locus_tag="AC16Y7_04125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014283311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR5708601.1"
                     /translation="MVYFISQVPDPLLATLDDRPPNVTVLAADGTVLAERGLRRGHIR
                     VDVLPDHLIKAVMATEDRRFYHHLGIDPVGLARASYRNFTADTVVQGGSTITQQLAKN
                     LFLKPDRTITRKLEELIYAVWLEQRFSKDEILELYLNRVYFGGGTYGVEAAARHYFGR
                     SARDVSLAQSAMLAGLLKAPSRYAPTRSVRAASTRAQVVLDNMVEAGFITEATARKVA
                     AQPLKLRAKGDDTGFPYAVDWVAELLPEYVGENDGALVVNTTVDAHLQRAAQERLRKL
                     LDTEGRKVKAGQGAVVILDPASGAVMALVGGRSYKASPYDRALKALRQPGSAFKPFVY
                     LAALESGYTPDSVAYDGPVNIRGWQPKNYTNKYKGKVTLRYSMAHSLNTVAVKLTSAV
                     GPERVAHTARRLGVNSKLHTQPSIALGTAEVTLLELAGAYAPFANGGKRVLPHVITRI
                     RNGEGKTLYKRRKSVTGQVVALQNVGAMNDMLNTALVRGTGKRAAIPKHMAGGKTGTT
                     QNSRDAWFVGYTAHYVAGVWVGNDNGKHMKKVTGGTLPASLWHDIMLSAHEDKRPLPL
                     PGAHSRRPREETIAVRLPWPSQTAKARDDATERPFYQRVFGFFGGG"
     gene            complement(17914..18756)
                     /locus_tag="AC16Y7_04130"
     CDS             complement(17914..18756)
                     /locus_tag="AC16Y7_04130"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR5708602.1"
                     /translation="MLKRLNLGRGETIEVKIQGLEIPMLLRRSTRARRFSLQVSEARR
                     GAVLTMPAYSSFADADEFLSRHLDWLKERVADLPEPVPFTDGAVIPLRGLAHLLRFAG
                     PVRRRGVVWVEDPEDAMIAPAWPVGARVAKERLPRLHISGEDEHAPRRLLDWLRRQAH
                     LDLKLRVDIHARRLNLQPKRIFVRDQTTRWGSCSTTGALSFSWRLVLAPPFVLDYLAA
                     HEVAHLAHMNHGPRFWAVVARTMPRHEEARDWLGKYGSNLHAYGAEAMPHHSQGTSPA
                     DSIE"
     gene            complement(18846..20117)
                     /locus_tag="AC16Y7_04135"
     CDS             complement(18846..20117)
                     /locus_tag="AC16Y7_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HIR5708603.1"
                     /translation="MLYHLYELNQAALHPARATAEAYRLMWRNPLNPASRTALGRGAA
                     AALELFERSTRRYRKPAWGIDTVEVDGREVGVVLRTVLSKPFCNLVHFERDLMGSRRR
                     KDPKVLVVAPMAGHFATLLRGTVRDLLPDHDVYVTEWQDARFVPRAAGAFDLDVYIDY
                     ITEFLRAFEGDVHIMAVCQPTVPVFAAVSLMEAQKDRAVPRSMTLMAGPIDARQSPTA
                     VNRFADGKPLSWFRQHVITQVPWPYPGMMRLVYPGFFQLSGFMGMNIDRHLTAHRDLF
                     HNLIRGDGDSADKHREFYDEFLAVMDLTAEYYLQTIETVFLDYKLPKGEMRHRGTPVD
                     PSKVKRVALMTVEGEKDDISGIGQTEAAQTLCSNLSDGLRAHHLQPGVGHYGVFNGSR
                     FRSDILPEIRRFIRAHQRRKSALGRALSRVA"
     gene            complement(20226..20825)
                     /locus_tag="AC16Y7_04140"
     CDS             complement(20226..20825)
                     /locus_tag="AC16Y7_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR5708604.1"
                     /translation="MRRPLLLVILGGFVIGALLGAVMLLNTDAQRTRVVTSGKALIGG
                     PFELAGKDGKTVTDKDFRGRYMLVFFGFTHCPDICPAELQVMSAALEDLGADADKVVP
                     IFITLDPERDTPEAVTAYVQNFGTNFVGLTGSPEAIEKAAKAYRVTYQKFQDESMGED
                     YSIDHSALVYLMGPDGAFVTHIPYGTPPKKMAETLRRYL"
     gene            complement(20828..21580)
                     /locus_tag="AC16Y7_04145"
     CDS             complement(20828..21580)
                     /locus_tag="AC16Y7_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5708605.1"
                     /translation="MYLLRSSGVRIVELIYWPFLQMLSWGFLQKYLASTENPYAQAAG
                     VLIGSVLLWDILFRSKIGFSTTFIEEMWSRNLGNLLISPLRPFELVAALSVWSIIRLS
                     VGMVPVSIAAFLFFGFNLLDLGLALAAFFFVLVLTSWSLGLIAAGVILRYGLGAEELA
                     WSLAFLLLPLCCVYYPVSILPDWLQPVALALPPTHVFEGMRSILLHGTFDPADLWWAL
                     GLNAIYLVAGYATFSWFLSSARENGTLLQLGE"
     gene            complement(21679..>22047)
                     /locus_tag="AC16Y7_04150"
     CDS             complement(21679..>22047)
                     /locus_tag="AC16Y7_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5708606.1"
                     /translation="TGKLSAGPKTRVGLAKALLNAPELLLLDEPTASLDPDTADWVRR
                     KLKSYARERKATLLLASHNMAEVERLADRVILLQAGRIIEDETPQALIEAHGRQTLED
                     VFLDVVRGRNAQAVPESLAS"
BASE COUNT         4255 a         6988 c         7011 g         3793 t
ORIGIN      
        1 tcgccttgcg gggacgccgc cttcaggccg gccacgcgat cgccgtcctt gatcagctcg
       61 tccacttccg cctgcatacg caaatgaaag ccgggatatt gctttgcctt cagggcaaga
      121 aaatcgagga agtgccattg cggcatgaaa acgatgaact tgcagaccgt cggcaggtgc
      181 gtgaagtcgg cgatgcggac catctctccg ccgatctccg acccgagcgt tttcacctct
      241 tggtgcggcc gggtgagaaa ttcatcgagc accccaagct cgtgcatgac ttgcagggtc
      301 gaggggtgga tggtgtcgcc gcgaaagtcg cggaagaagt cttcgtgctt ttccagcacg
      361 acgacatcga ccccggcgcg ggccagcaga tagcccagca tgattccggc cggaccgccg
      421 ccggcgatgg cgcatttggt ggtgatgctc tccgtggcca tggcgttccc tcaatgccgc
      481 gtgcggggcc actcctagcc agcatgccgc cgcaaccttc ggctgaaaag atttagaaac
      541 cctatggggt ggctgcgggc gcggcggccg tggcgcctgc gcgatgatgg ctgagatcgc
      601 gaaaccttgg atttctgccg ttcagggggt tttccgggtc ccatccatag gcgacggtcg
      661 agaagcgtgc ggagagcgca tcgtagagaa ggagccggcc caccagcccc tcgccgatgc
      721 cgagcagctc cttcaccacc tcgaccgctt ggaggcagcc gaccacgccc gccacggcgc
      781 cgagaattcc ggcttcggcg cacgccggca cggtgccctc cggcggcgga tcgggaaata
      841 ggcagcgata gctggggcgg gagttcccgt tctcgtccgc ctcgaaggcg cggaaggtgg
      901 tgagctgacc gtcgaacggc cccacggcag ccgtaaccaa cggcgccttg gcgaaatagc
      961 acgcgtcgtt gacgagatag cgcgtggcga aattgtcgga tccgtcggcg acgatgtcga
     1021 aaccccgcac aatatcgagc gcgttctccg caacgaggcg cgtagagaag agcgtcgtct
     1081 ccacatgcgg gttgaggcgt tcgagcatgg ccgccgcgct tttggtcttg aggcggtcaa
     1141 cgctttccgt gttgtgtgca atctggcgct ggaggttatc gacactgacg cggtcatcgt
     1201 cgatgagacc gatctgccca acgcccgccg cggcgagata cagcatcagc ggtgagccaa
     1261 gaccgcccgc gccgacgatc agcacgcgcg cggccttcaa gcgctgctgg ccttcgccgc
     1321 cgatctgctt cagcaccaga tggcgccgat agcgttcgat ttcctcgtcg ctgagcatgg
     1381 cttcaatcta tgccttgcgt cgcctcgcgg ccagtcaggc gcgcctagcc cgtggagcca
     1441 aagccgccgg cgccgcgctc cgtctcgttc agcgcatccg cctccacgat ctccacttgg
     1501 gtgaccggcg cgacgatagc ctgggcaatg cggtccccgc gtctaatcaa gaacgggacg
     1561 ttgccgagat tgatgagcag cactctcacc tcgccgcgat aatccgcatc aatggtgcct
     1621 ggactgttca gcacggcaat gccgtgcttt agcgtgagcc ctgagcgcgg gcggatttgc
     1681 gcctcgtagc cggcggcgag ctcgaatacg agccctgtcg gcaccagcac acggtcgcta
     1741 gggtcgagcg taatggggcc gtcttcaggg acggcggcga gaagatcgaa gcccgcagca
     1801 tgagccgatt gataggcagg gagcggcaac tcttcggcgt ggggtaggcg caaaatccga
     1861 agcgtggcgc tcattcggcg gccgcgctca acgtggtgag agcttcgccc gcgcgggcga
     1921 gcagctttgc tgccatctcg tccttgctca tggcgggcca gtcctcgacg ctcgtgccgg
     1981 tgacaagatg cacttccgtg cgatcgccgc ccataatgcc ggtgccggcc gacacgtcgt
     2041 tcgcgacgac ccagtcggca cccttggcgc ggcgtttgtc gcgcgcgtgc tcgacgacgt
     2101 cttgcgtctc cgcggcgaaa ccgatcacga gatcgggcct catgggtcct tcgaccgaaa
     2161 tggtggcgag gatgtcgggg ttcttggcaa gggcgagcga caactccgtc acgttgtctt
     2221 gcttcttgag cttctgagaa gcttcggcct gcgcgcgcca atcggcgaca gcggcggcgc
     2281 agatcgcgat gtcggcaggc agtgcatcga tcaccgcatc caacatctcc tgcgcggtct
     2341 ctaccttgat ggcggtgacg ccgtcgggcg cgggcagcgc gacgggaccg gagatcagcg
     2401 taacgcgcgc gcccaactct accgacgcgc gggcgagcgc atggccctgc ttgccggagg
     2461 accggttggc gatgtagcgc accggatcga gcggctcatg tgtcggaccg gaagtgatca
     2521 atacatgccg ccccgagagc ggcccgctcg gcgcggccgc aagcgatccc tcgatcgcgg
     2581 caacgatcgc ctccgactcg gccatacggc cgggcccgtg ttcaccgcac gccatgtcgc
     2641 cgtcatccgg tccaacaaaa tgaatgccgt cggcgcggag cgtcgtgaca ttgcggcgcg
     2701 tcgccgggtg ctgccacatg cgcacattca tggcgggcgc caccaaaaca ggcttgtcgg
     2761 tggcaagcag cacggtcgag gcgagatcgt tggcaagccc tgtcgccatc ttggcgagaa
     2821 tgtctgccgt ggcgggcgac accacgacga ggtcggcctc gcgcgacaag cggatatgac
     2881 cgatctctgc ttcgtccttg aggtcgaaca ggtcggtgaa ggtcttgtcc ccggagagta
     2941 tggagaagga aagaggcgtc acgaattccg cgccgccttt ggtcaggatc acgcgcacgg
     3001 acgcgccccg ctcggtcaag cggcgcacga gctcgaggct cttataggcg gcgatgccgc
     3061 cgccaacgat caaaaggata cgctttcctt gcacgagtct ctttcctggc tctggggtcc
     3121 tttagtctac cacaccgcct agagcagcag cgccagcgcg atagcgacga gcgcggccgc
     3181 gccgacccag atgccgacac ggctccagcg gtcgcgcgta tactgggcct ccgccaagcg
     3241 ctccacgctc tgatcgtcca ggcggagccc gtcgcccacg atggctccga aggcgtccac
     3301 ggtgcgctcg gcttgcgtca ggagccgcgg cagatcgccg acgaagcggc ctacggacgc
     3361 tgcgccctcg gccgcctgct ccagccgtgc accggcgccc agctggctct ccagccactc
     3421 cttcaccaca ggctcggcca cgacccacat gttcagttcg ggatcgaggc tgcggcccac
     3481 gccttccacg acgaccatgg tcttttgcag catgatcagc tcgggccggg tctgcatgtc
     3541 gaagacctcg gtgtactgga ggagctggcc cagaagatgc gccatggaga tttcgctggc
     3601 aggacggtcc atgatgggct caccaatggc ccgcaaggct tgcgcgaact gcgccaccga
     3661 atgtttgcgc ggcacatagc ccgcatcgaa atgcacctgc gccacacgca tatagtcgcg
     3721 cgtgatgaag ccgtagagaa tctcggcgag gaagcgccgc tccttcatgc cgagccggcc
     3781 catgatgccg aagtcgacgg caacgatacg cccttcgccg tcgacgaaca gattgcccgg
     3841 atgcatatcc gcgtggaaga agccgtcccg caaagcatgc cgcaggaaag tctgcatgag
     3901 ccgcgcgccg agtggcttga ggtcatgacc agccgcgcgg agcttgtcgt ggtcggcgat
     3961 gggaacgccg tcgatccatt ccagggtcag cacgcggcgg gcactgcgcg tccagtcgac
     4021 tttcggcaca cggaagcctt cgtcgtcctt cgagttttcc gccatctccg agagcgccgc
     4081 ggcttccatg cgcaggtcca tctcgatggc gacggacttc gacagcgtgt ccaccacggc
     4141 gataggccgc aagcgccggg acggcgaatg gaagcgctcg atcaggcgcg cggcgaagaa
     4201 ataggaatcg aggtccttct taaaacgctg ctcgacgccg ggacgcagaa ccttcaccgc
     4261 aacgtcgctc aatgtgccgt ccggtgcctt caccttggcc ttgtgcactt gcgcgatgga
     4321 cgcagccgcg accggcgggc cgaactcggc gaagagctga ccaatgggcg cttcgagttc
     4381 cgtctcgact gccttgactg cttcttcgtg tgagaacggc ggaagtcggt cttgcagcgt
     4441 ggagagatcg cgcgccagct cacggccgat aatgtcgtcg cgagtggcga ggaactgtcc
     4501 aagcttgatg taggagggcc ctagcgatgt cagcgcggtc gacaacctcg cctcgttgcc
     4561 ggaagcggcg ccgttctgcc gcaagggggc cgtgaccctg ccgaacacgg caagcggccc
     4621 ggacagtttg gtgtccgcgg gcagtacccg cgcgccgctc caggcgagcg ttgcgcccgc
     4681 cttgggtagc cgcgccatat tcgcgatggc gttcacttat cgctgcctca tctaaagccg
     4741 ccaagcggcg tggatcgccg cgatcccgcc ggtcaggttc cgatagcgca cgcgctcgaa
     4801 ccttgcatct cttatcatct gcacgaagcg ttcctgatcg ggaaagcgtc ttatgctttc
     4861 caccagatag cggtaggact ccgcgtcgcc agcgacaagc tggccaagct gcggaatggc
     4921 attcaaggaa taggcgtcgt agagcgtgtc gagcagtggc atctcgacgc tcgagaattc
     4981 caagcacagg aaccgcccgc cgggcttcag cacgcggaag gcctcgcaaa gcaccttatc
     5041 gatatgggtg acgttgcgga taccgaaggc aatcgtatag gcgtcgaagc tgcggtcttc
     5101 gaaggggagg ttttcggcat tggcttgcac gaattcgagg gccctgcgcc cgccagcgcg
     5161 cgccttcccc gcctccatca tctcggcgct gatatcgcag atcacggcgc tgccgccccg
     5221 ccccgccgca tcgagaaagc ggaaggccac gtcgccggtg ccgccggcca cgtcgagcaa
     5281 ggcaaagggc gtttcgccct tgggcggagc gagcgcgccg atgagctcgt ccttccatag
     5341 gcgatggagc cccccggaca tcagatcgtt catcaggtcg tagcggggtg cgaccttgga
     5401 aaacacttgg tttacaaggt cttggcgcgc gccctcggcc acgcgggcaa agccgaagct
     5461 cgcctcagtg tccctggggg ctgtgtcttt gtggtcttcg ggcatggcag cggacaatag
     5521 cctaagccaa aagttcaggc tattgttgca gtgcaatgag gttattctgc gctgcaatat
     5581 gcagggacgg ccatgcctga gcttcctgaa gtggagaccg tccgccgtgg gctggcgccc
     5641 attctcgtgg gcaacagctt cgcccgcgtg gagcagcggc gtcccgatct gcgctttcct
     5701 ttgcccgagc ggttcagtga gcggctgcaa gggcggacga tcgaagcgct cgaccggcgc
     5761 gcgaaatatc tcttggcccg gctcgacgac ggcgaggttc tgatcatgca tctgggcatg
     5821 acggggcgct tcgccatcga caaggcgaac ggcgtgggcg cgccaggccg catcgcacgc
     5881 gcgccggctc aaacaccgaa gcacgagcat attgtcttcc atctgcgcga cggctcggcg
     5941 gtgcgctaca acgacgtgcg ccgcttcggg atcatggatc tcgttccttc gagctccctc
     6001 gacagtcacg cattgttcaa aagcctcggg gtggaacctc ttggcgatgc gctgacctcc
     6061 gaatggctcg cgggaaagct caaaggcaaa gccacgtcga tcaaagccgc cctgctggat
     6121 cagcggctga tcgcggggat cggcaacatc tatgcgtgcg aggcgctgca tcgcgcggcc
     6181 atctcaccgc tcaagctcgc cggtgcgctc acaacaaaga ccggcaagcc gacaaaaaag
     6241 acggacgcgc tcgttcgggc gatcaaggcg gtgctggaaa gcgccatcaa agccggcggg
     6301 tcgtcgttgc gcgactatcg ccacaccgac ggccggctcg gacgtttcca gcatcgattc
     6361 aaggtctatg gccgcgaggg caagccgtgc ccacggacgg gatgcggcgg cacggtccgc
     6421 cgcaacgttc aaagcggccg ttcgaccttc ttctgttcaa cctgccaaaa atgagggact
     6481 gacatccatg cttgccgagg acatcgcaca aggcgaggaa gcgcccgccc agaaccctta
     6541 tcagacgttg atcgtcacgc gagagggcaa ggtcgggctg atcacgctca accgtcccaa
     6601 ggcgctgaac gcgctcaata cgcagctcat cggagagctg aacaaggcct tgaaggcatt
     6661 cgacaccgat ccagaaatcg gctgcatcgt catcactggc aatgagaagg cgtttgccgc
     6721 cggcgtcgac atcaaggaga tgcacaccaa gtcgttcgtt gaggcgtatg gcagcgactt
     6781 cttggcgccg ttcgacaaga tcaacaatat ccgcaagccc atcatcgccg cggtcggcgg
     6841 gtatgcgctc ggcggcggct gcgagcttgc catgtgctgc gacatcatcc tggctgccga
     6901 caatgccgtg ttcggtcagc cggagatcaa tctcggcatc atgccgggcg caggcgggac
     6961 gcagcgcctg acgcgcgccg tgggcaaggc caaagcgatg gagctgtgcc tcaccggacg
     7021 ccagatgaat gccgaagagg cggagcgcgc gagcctggtg agccgcgtgg tgcctgccgg
     7081 tgagctgctc ggcgaggcca tgaaggtcgc ggccaagatc gccaatctgt cccagcacgc
     7141 ggtgatgatg atcaaagagg cgatcaaccg cgggttcgcc acgacgctcg ccgaaggcac
     7201 gcgcttcgag cgcggccagt tctactcgct gttcgcgacc gaggaccaga aggaaggcat
     7261 gaccgccttc atcgacaagc ggaagccggc cttcaagaac cgctaggagc cggtgaggct
     7321 tgcccttcgc cgcacgatct tccaagctct tgggaaggga gttcgccgat gcgcgggaag
     7381 gtgacagtca ttgcgtttca tcgagccaag cccggcaagg agcgcgcctt acgcgagacc
     7441 ctgctggcgc tgtgtggtcc gacgcgcgtg gagaagggct gcataaatta cgacctccat
     7501 gtttccgccg atgatcctag ccttctggtc ttccacgaga actgggtctc caaggcggat
     7561 ctcgaggcgc acctcgcctc cccgcatatc gacgccttcc gggagattgc cggggaactg
     7621 ctcgccgaac cccctgacat caccctatgg agcgaggcgg aggcttaagg gccctccagg
     7681 agccttcggg cgcctcggcg ccatagttga cgctttgggg ggcgctcggt atagagcact
     7741 caaatttcgc gtttccggca cttttgagag ccgcaaaacg gcgaaccgaa agtagccctc
     7801 aaggacccta aatatggcca ataccaagtc ggcgaagaaa gccgttcgcc agatcgcccg
     7861 tcgcaccgaa gccaacaagg ctcggcgcag ccacatgcgc agttcaatcc gcagagtgga
     7921 ggaggcgatc gcgacaggca gcaaggaggc cgctcaggca gccttgaagg aagccgagcc
     7981 gatcatggcg cggacggccc agaagggcct gctccaccgc cgtgccgcgt cccgcaaagt
     8041 gtcccgcctc gccaagcgcg tcagcgcgat gggcgcgtag ccggacccgc tcaaaatcac
     8101 acgcgcaata tttgttgctt aagtgccaca gcgtttgcac gcgtgtctgc gcgcgcgttc
     8161 gcgcttcatg tcaatttgac gactatttca atcgcttgac gattggcagc gccaacatga
     8221 cagattggtt ttccaccaac ttccctctga aaaaaaaatt tgaggttgca ctgcctccaa
     8281 aagccctgtc cgcagaggga tcggccctct agctccgtcg attcgcgctt tcgacacaga
     8341 ggcttgcgga tcgcgcgtag tcggaccgct tactgatcaa tactgtcgct cgcgcccctt
     8401 gcatacgctt gattgctggg gtttccgtgt gtatagtctg gttaaccgaa cggggaagtc
     8461 ccctccttac aattgagtat cttggccttg gcgcttgctg ggacaggttt tatttgtctt
     8521 cgcagtaagg ggggatgaat atcttgtgga atgcttatgc gtaatctgtg agtatttcgg
     8581 gtgtaagttt cgtgaagtca gcacgaattg tggttggcac cgaaactgtt cttaagacgt
     8641 gctcaaactt ttgcgggagg aggctcgatc ttgggcgcgc agtcggatga aaacccggtc
     8701 gacgaggttg atgtggcaac ggcctggaat ggcctcaagg caacccctcg atcacaactc
     8761 gtcgatgtgc gcacccgtgc ggagtggacc tatgtaggga tccccgatct tgtgccgttg
     8821 ggaaagcgga ctgtactcgt tgagtggcaa acctttcccg atcagtccgt ggatccgcgc
     8881 tttgccgaac gccttgccaa tgagttgacg gcgctcggtg tccagttaga cgacgacctc
     8941 ttctttattt gccgttcggg tagccggagc ttggccgcgg ccaaggcgat ggcggcggct
     9001 gggtaccgtg cgtgtcacaa cgtgtcagag gggttcgagg gaccgctcga cgacgcaagg
     9061 catcgcgggg cccaattggg ttggaaagcg attggacttc cctggcagca aagctaggga
     9121 ccgatcgaat gggacggttg tggctgtccg cgtcgatcaa gttggctgct caagtaacat
     9181 gcgtgcggtg cgtaatggcg cgacaaaaaa tgaacaggaa agaacaacac cgtggcagag
     9241 gcacaagcac gggctcatca cgagccagag gagcagtgcg caccaagcga agcttggacg
     9301 agagtaaagg cccgcttacg tggggaagtg ggggaggacg tctacgcaag ctggtttaag
     9361 ggcgtggagg tccaagaggt gggcgaaggc gttgtccatc tcaccgtggc gacacgtttt
     9421 ttacggaact ggctgcgcac ccactactac gaatccgtct tgaggcattc gcgcggcgag
     9481 tggcccgcgg ctgaacgcct tgagttcacg gttcggcagc cgcattttgc ggtcgagccg
     9541 gtcaaggacc cgccacgcgt acagcgcgcg ccggcgcccg ccgcaaacga ccggacgctc
     9601 ctgccgatgc cgttgcgcac cggctatgag ggcttcgaag gctcgcccct cgacccgcgc
     9661 ctcagcttct ccagtttcgt cgtgggcaac acgaacaagc tggcgcacgc agcggcacag
     9721 caggtcgcac acgcttcgtc gagtccgcag tcttcattca atccattgtt tttgcacggt
     9781 aatgtcggcc tgggcaaaac ccatttgttg cacgccatcg cctgggaggt cacgcagcag
     9841 aagcccgagg ccgaggtgct ttacctcacg gccgagcgct tcatgtccgg tttcgtgcaa
     9901 gcgttgcggg cgcgtgatgc actggccttc aaggagaagc tccgcaacat cgacatcttg
     9961 ctgatcgacg acatggagtt tctgcaaggc ccgaccatcc agcaggagtt ctgccacacg
    10021 ctgaactcac tgatcgacgg cggacggcaa gtggtcgtcg cggccgatcg cgcgccgacc
    10081 catctcgaca agctcgacat gcgcatgcgc tcacggcttg gcggcgggct cgtggccgag
    10141 atcaaggcca tggactacga tctgcggcac aagattctgg agaagcgggc gcaagaggcc
    10201 tgcaccgaga cgaacggact ggaagtatcg gacaccgtgc tcgccttcct tgccgaacgc
    10261 ctgagcgaaa gcgggcggga actcgaaggc gcagttcacc gccttagggc gagctatcag
    10321 ctcaccgacg agccggtgac gatcgccatc gccgagcaga tcgtgcgcga tctgatgcgg
    10381 ggcgcggagc cgcggcgggt gcgtatcgac gacatcctac ggacggtgtc gaagcattac
    10441 ggcgtcaacc gcggcgatct cctgtctgga cggcgcaacc gctcgatcgt gcggccgcgt
    10501 cagattggca tgtatctcgc caaactcctg acctcccgct ctctgccgga gattgggcgg
    10561 cggttcggca accgcgatca caccacggtg ctgcatgcga tccgcaagat cgagcagctg
    10621 atgagcgacg acaaccagct tcgcgaggag atcgaactgt tgaagcgtct gctgcgcgac
    10681 tagggggaaa ggcaggaggg gccttgcgct ttggcctgag gcctctcccc tgctcttgca
    10741 aatttcgcgg cgtcgcgcca agttcgactg ggctttccgc ccgttttccc taggcctcca
    10801 ccaaggccgg accggagcag aaagatgctt ctggctggga aacactcggc gagaaaacga
    10861 accaattttc ggggtattct ccaaccatgc gcctcaccat cgacaagccc ggtctggcca
    10921 agtcgctcgc ccacgtggcg agcgtggtcg aacggcgcaa caccatcccc attctttcga
    10981 atgtgctgct cacggcggac gcgaacgagc tgaagctgac cgcgaccgac ctcgacatgg
    11041 agctcgtgga acgggtggcc tgcaccgccc aaggcgacgg cgcggtcacc gtgccagccc
    11101 atatgttcta cgacatcgtc cgcaagctgc cggacggcga gatcgagctc aatcgcgatg
    11161 cggagcaaga gcgtctcgtc atcaccagcg gtcccgcgca gttctcgcta caaacgcttc
    11221 ccgccgacga ttttccgtcg ctcagcgtgg aagacctcga ccatgccttc aaggtcccgg
    11281 ccggcgatct caagcggctg atcgagaaga cgcgcttcgc catctccacc gaggagacgc
    11341 gctactattt gaacggcatc tatctccatg aggccaaggg tgagagcgag ggcaaaccta
    11401 cgcttcgggc cgtggccacg gacggacacc gtctggcgca agtcgagttg ccgctgccat
    11461 caggcgctgc cggcatgccc ggcgtgatcg tgccgcgcaa aaccgtgcag gagctctcca
    11521 agatcgtgga ggacgaggac ggcgaggtga ccattgagct gtcaccgtcg aagatccgca
    11581 tcgccgctgg ccgcgtggtg ctgacctcca agctgatcga cggcaccttc cccgactacg
    11641 agcgcgtcat cccgcaaggc aacgacaagc agctcgaggt ggacaacgag accttctcga
    11701 aggccgtgga ccgggtgtcg accctgtcca gcgacaaggg ccgcgctgtg aagctcgcgc
    11761 tggccgacgg cacgctgacc ctgtcggtga acaatcccga atccggcagc gccactgaga
    11821 aactttctgt cgaatacgaa cacgatccgc tcgagatcgg gttcaacgcc cgctacctgc
    11881 tcgacatttc cgatcagttg gagggcgaaa cggccgagtt tcatctggcc gatccgggat
    11941 cgccgaccat ggtgtgcgat gtaaaggatc catcggcgct ttatgtcctc atgccgatgc
    12001 gggtatgaac gcaacgccgc ggcagcacac acccgaggat gcgcacgcgt cccagcctga
    12061 cctgacgcgg cttgccgtcc gcacgttgaa gctcaccaac tttcgcaact atgcaagcgc
    12121 gcgcctcgat ctcgatgcgc ggcccgtggt gctcaccggc gacaacggct cgggcaagac
    12181 caacctgctg gaggctgtgt cgttgctggg tcccggccat ggcttgcggg gccgccccta
    12241 tgtcgagctt gcacgcaaag acggggacgg cggatttgcc atcgccgcca cggtgatgac
    12301 gcgggctggg gaaacagaaa tcggtaccgg gctgcttgcc agtgcgcgcg aagagggcac
    12361 gggccgcacg gtacgcatca acggcaagga ccatagcgcg ggcgccctcg gcgagtatgt
    12421 gaagcaggtc ttcctcatcc ccgccatgga cggactgttc acgagcggcg gatcggaacg
    12481 gcggcgcttt ctcgaccgcc tcgtgctttc catcgacccg ctgatgcgca cgccgctgaa
    12541 ccgctatgaa cgggccatgc gccagcgcaa ccggctgttc cagatgcggg agcattcgcg
    12601 cagtctcttc gagggcctgg aggagcagat ggctgaagcc ggggtcgcca tcgccgccgc
    12661 gcggtacgac gccgtggagc ggctcaccgg cctgatcgag gagacacgag aagcccaggg
    12721 cgaaggcccc ttcccgcacg cggtgctggc gcttcacggc acgctggaat cggagctgac
    12781 cgagcggccc gcaacggagg ttgaggacgt ctttcgcatc ctgctggagg acggccggga
    12841 gcgggaccgt gcggcgtcgc ggacccttga aggtccgcac agaagcgacc tattagtggt
    12901 tcacgggcct aagaacgcgc ccgccgccca gtgctcctcc ggcgagcaaa aggcccttct
    12961 gctgggtctc atcctcgcca aggccgagct catcaagtcg ctcgaaggca acgcccccct
    13021 tatcctcctg gacgaggtcg cggcccatct cgatgcggcc cgcagggaag ccctttttgg
    13081 cgagattttg cggctcggag tgcaggcctg gatgaccgga acggataagg agcttttttc
    13141 gccgatcgcc caagacgccc aaatctttgc tgtgaatcag ggtaatatcg aagcctgaat
    13201 caggggcctc tcgggcctcc caaaaagacc cattactgcg accttcaaga acacctcagg
    13261 acaccatgac accagctgcc aaacgcaaag ctgaagctgc cgcagactac ggcgcagagt
    13321 cgatcaaagt gctgaaaggc ctcgacgccg tgcgcaagcg gccgggcatg tatatcggcg
    13381 acaccgacga cggctccggc ctgcaccaca tggtctacga ggtggtcgac aacgccatcg
    13441 acgagtcgct cgccggctat tgcgacgaga tcaacgtcat gctcaacgcc gacggctcgg
    13501 tgaccgtgga agacaacggc cgcggtattc ccaccgagat ccacaagggc gaaggcgtgt
    13561 ccgcggccga agtgatcatg acgcagctcc acgccggcgg aaagttcgac cagaactcct
    13621 acaaggtctc cggcggactg cacggcgtgg gcgtgtcggt ggtgaacgcg ctgtcgagca
    13681 tgctggaact caccatttgg cgcgacggca aagagcacac gatgcgcttt gccgacggcg
    13741 tgcccgaggt gccgctgaag cccatgcgcg actcgcaagg gcggaccggc acacgcatca
    13801 ctttcgtgcc gagcacgaac accttcacca tggtcgactt cgattacgcg acattggagc
    13861 atcgcttgcg cgagctcgcg ttcctgaact ccggcgtacg cattatcctg tcggacttgc
    13921 gcggcgtcga acccaagacc gaggagatgc actacgaagg gggtttacgc gccttcgtgc
    13981 agttcctcga ccgcaccaaa catccgctca tcaacgagcc ggttgtcgtg gagaagatgc
    14041 aaagccgcat cacggtggaa gcggccttgt ggtggaacga cagctaccac gagaacgtct
    14101 tgtgcttcac caacaacatc ccgcagcggg atggcggcac gcatttggcc ggcttccgcg
    14161 cggcgctgac ccgcacggtg aacacttacg ccgcggcgtc cggcatcgcc aaaaaagaga
    14221 aggtggcgct cacgggcgac gatgcgcgcg agggactcac ttgcgtgctt tcggtgaagg
    14281 tgcccgatcc gaaattctcc agccagacca aggacaagct cgtctcctcg gaagtgcgcc
    14341 cggtggtcga gagcgtggtg gccgagcggc tctcccaatg gttcgaggag aacccgggcg
    14401 aggcacgcaa tatcgtgcag aagatcgtgg aagcggcggc cgcgcgcgaa gcggcgcgca
    14461 aggcccgcga actcacgcgg cgcaagggcg cgctggacgt ggcctcgctg cccggcaagc
    14521 ttgccgactg tcaggagcgc gatccggcca agtccgagat cttcctggtc gagggcgaca
    14581 gcgccggcgg cagcgccaag caggcgcgcg aacgcgcgtt ccaggcggta ctgcccttgc
    14641 gcggcaagat cctcaacgtg gagcgcgcgc gcatcgacaa aatgctgtcg agcgcggaga
    14701 tcggtacgct catcaccgcg cttggcacaa gtatcggcga tgagttcgac ctagcgaagc
    14761 tgcgctatca caagatcatc atcatgacgg acgccgatgt cgacggctcg cacatccgta
    14821 cgctgttgct caccttcttc taccggcaga tgccgtcgat catcgaggcg ggccacctct
    14881 atatcgccca accgccgctc tacaaggtga agcgcgggaa ccatgagcgc tatctcaaga
    14941 acgaagatgg tcttgaggac tacctcatcg gcgaggggct tcaggacacc gtgctgacga
    15001 ccggcgagga cgccgcgcgc ggcggcgggg acttgcgcga catcgtggag caggcgcggc
    15061 tcgttacggg catcctgcgt gggctgcata gccgctatcc gcgtttcgtc atcgagcaag
    15121 cggccatcgc gggtgccctc agtccggacg tgctgaagga cacgaagcag gcgcaggccg
    15181 cggcggccta tatcgccaag cggctcgact cgctgtcgga agagaccgag cgcggctggc
    15241 acggcgaagc gctgcccgaa ggcggacttc gcttctggcg cgagctgcgc ggcgtgatgg
    15301 agtctcatct gatcgacggg ccgttcatcg cctccgccga tgcactgaag ctcgatcagt
    15361 acgccccgca cctgcaagag gtctacgcca agccggccac cctcaagcga aaagacacga
    15421 gcctggaagt ccacgggccc tcggaactgc tcgatgcggt gatggagacc ggacgcaagg
    15481 gccttacgct ccagcgctat aagggccttg gcgaaatgaa cgccgaccag ctcttcgaga
    15541 cgacgctcga cgtcgatacg cgcaccatcc tccaggtgaa gatccgggag ggtgaggacg
    15601 ccgacgacat cttctcgcgg ctgatgggcg acgtggtcga gcccaggcgc gagttcatcc
    15661 aggaaaacgc cctggaagtt gccaatctgg acgtttagcg gcgcctccta cgcctccatt
    15721 ccttaacgct gtggcaaggc gggcccctta aggtttccgc gcgaagagcc ttgcgattca
    15781 ccatttttgg tggcgaggct caccttcgag ttcagttgag gcgttgagtt gagatgagcg
    15841 gcgtgcggcg aaaggccaaa ccccaaggca ggagcagcaa accggcgggc tcgcgtggac
    15901 ccacgcggcg ccggccacca tcgaagcggc gcccttccag ccggcgtccc aagggccgct
    15961 ttgcccgcct aagacgcacc gcccgggcgc atccttacct cttcaaagcc ctccgcttca
    16021 gccttttagc tgcgatctgg ggcaccatcg cgctcggcgg agcggtggtc tatttcattt
    16081 cccaggtccc cgatcccctg ctcgccaccc tcgacgaccg tccgcccaac gttacggttc
    16141 tggccgccga cggcaccgtt ttggcagagc ggggtttgcg ccgcgggcat atccgcgtgg
    16201 acgtgcttcc cgatcacctc atcaaggcgg tgatggcgac ggaggaccgg cgcttttacc
    16261 atcatctcgg catcgacccg gttggcctcg cgcgcgccag ctaccgaaat ttcaccgcgg
    16321 acaccgtcgt ccagggtggc tccaccatca cccagcagct tgccaagaac ctgttcctca
    16381 agccggaccg cacgatcacg cgcaagttgg aggaactcat ctacgcggtg tggctggagc
    16441 agcgcttctc caaggacgag atcctcgagc tttacctgaa ccgcgtctat ttcggcggcg
    16501 gtacttacgg cgtggaggcc gcggcccggc attatttcgg ccgctctgca cgtgacgttt
    16561 cgctggcgca gtcggcgatg ctggctggcc ttctcaaggc gccctcacgc tacgcaccga
    16621 cccgcagtgt gcgtgcggcg agcacgcgtg cccaggtcgt gctcgacaac atggtcgagg
    16681 cgggattcat cacagaggcg acggcccgca aagtcgccgc gcagcctctg aagcttcgcg
    16741 ccaaaggcga tgatacgggc ttcccttacg ctgtcgactg ggtggcggag ctgctgcccg
    16801 aatatgtcgg cgagaacgac ggcgcacttg tcgtaaacac gacggtcgac gcgcatctgc
    16861 aacgcgccgc gcaggaacgc ctgcgcaagc tgctcgacac cgaaggacgt aaagtgaaag
    16921 ccggccaagg cgccgtggtc atcctcgacc ccgcatcggg cgcggtaatg gcgctggtcg
    16981 gcggccggtc ctacaaagca agcccttacg accgcgctct gaaagccctg cgccagccgg
    17041 gctctgcctt caagcccttc gtctatctcg cggcgctgga aagcggctac acgccagact
    17101 cggtcgccta tgacggtccg gtcaatatcc gtggctggca gccgaagaac tacacgaaca
    17161 agtacaaggg caaggtcacg ttgcgctaca gcatggcgca ctcgctcaac acggtcgccg
    17221 tcaagctgac ctccgcggtc gggccagagc gtgtggccca cacggcgcgg cgtctcggcg
    17281 tcaattcaaa acttcatacg cagccatcca tcgccctcgg cacggcggag gtgacactgc
    17341 tggaactcgc cggcgcatac gccccgtttg ccaatggcgg gaaacgcgtt ctgccgcatg
    17401 ttatcacacg catccgcaac ggcgagggca agacactcta caagcgccgg aaatccgtta
    17461 caggacaagt ggttgcgctc caaaacgtcg gcgccatgaa cgacatgctc aacaccgcgc
    17521 tggtgcgcgg gaccggcaag cgcgcagcca tccccaaaca catggccggg ggcaagaccg
    17581 gcaccaccca gaattcacgg gatgcctggt tcgtcggcta cacggcgcat tacgtggcgg
    17641 gcgtgtgggt cggaaacgac aacggcaagc acatgaagaa ggtcaccggc ggcacactgc
    17701 ccgcctcgct atggcacgac atcatgctct ctgcacatga ggacaagcgg cccttaccgc
    17761 tcccgggtgc acacagccgg cggccgcgtg aggagaccat cgcggtgcgg ctgccgtggc
    17821 catcgcaaac ggccaaagcc cgcgacgacg cgacggagcg tcccttctac caacgcgtct
    17881 ttggcttctt cggcggcggg tagaactctt ctgctattcg atgctgtccg caggcgaggt
    17941 gccttgcgaa tgatgcggca tggcctccgc cccgtaagca tggaggttgg agccgtattt
    18001 gcccagccag tcccgcgcct cctcgtgccg cggcatggtg cgcgcgacaa ccgcccaaaa
    18061 gcgcggccca tgattcatgt gggcgagatg ggcaacctcg tgcgcggcga gatagtcgag
    18121 cacgaagggc ggcgccagga cgagccgcca cgagaaggac agcgcgccgg tcgtagagca
    18181 tgatccccag cgcgtcgtct gatcgcgcac gaagatacgc ttcggctgca ggttcaggcg
    18241 cctggcatga atgtccactc gcagcttcag atccaaatgg gcctggcgcc tcagccagtc
    18301 cagcagccgg cggggcgcgt gttcgtcctc tccggagata tgcaggcgcg gcaggcgctc
    18361 tttggcgacg cgcgcgccga ctggccatgc gggcgcgatc attgcgtcct ccgggtcctc
    18421 gacccagact acgccacggc ggcgcacggg gccggcgaaa cgcaagaggt gggcgagccc
    18481 gcgcaaggga atgacggcgc cgtcggtgaa cggcactggc tcgggcaggt cggcgacccg
    18541 ctccttgagc cagtccaaat ggcgtgacag gaattcgtcg gcatcggcga agctggaata
    18601 ggccggcatg gtcaggaccg cgccgcgccg tgcctcgctg acttgaaggc tgaaccgcct
    18661 ggcccgcgtg ctgcgccgca gcagcatcgg aatttccaag ccctgaattt tgacctcgat
    18721 cgtctcaccc cgcccgagat tgagccgctt cagcatcccc gtctccctta cgccacacct
    18781 tgagggctac ccgaatctgc gccttcagcg tagcacaggg gctcgcaaaa acgctcgcct
    18841 gtggtttagg caacgcggct caaggcccgt cccagagcgc ttttgcggcg ctggtgagcc
    18901 cggataaaac ggcggatttc cggcaaaata tccgaccgga agcgcgaacc attaaaaacg
    18961 ccgtagtggc cgacgccagg ctgcaaatgg tgggcgcgga gaccgtctga gaggttgctg
    19021 cacaatgttt gggcagcctc agtctggccg atcccggaaa tatcgtcctt ctcgccctcg
    19081 accgtcatga gcgcgacccg cttgactttc gaggggtcca cgggcgtgcc ccgatggcgc
    19141 atttcgccct tgggcagctt gtagtcgagg aacaccgtct cgatggtctg aaggtaatac
    19201 tcggccgtga ggtccatgac cgcgagaaac tcgtcgtaga actcacggtg cttgtcggcg
    19261 gaatcgccgt cgccgcggat caggttatgg aacagatccc ggtgggcggt caggtgccgg
    19321 tcgatattca tgcccatgaa gccggagagc tggaagaagc ccggatagac gagccgcatc
    19381 atgccgggat acggccaggg cacctgggtg atgacgtgtt ggcggaacca gctcagcggc
    19441 ttaccgtcgg cgaagcgatt gacggctgtc gggctctgcc gcgcatcgat gggaccggcc
    19501 atgagggtca tggagcgggg cacggcgcgg tccttctgcg cctccatcag cgacacggcg
    19561 gcgaacaccg gaacggtcgg ctgacagacc gccatgatgt gcacgtcgcc ctcgaaggct
    19621 ctcagaaact ccgtgatgta gtcgatgtag acgtcgaggt cgaaggcccc tgcggcgcgc
    19681 ggcacgaagc gcgcgtcctg ccattcggtg acatagacgt cgtgatcggg gaggagatcg
    19741 cgaactgtgc cgcgcagaag cgtcgcgaag tgacccgcca taggcgccac cacgaggact
    19801 ttcggatctt ttcgccggcg cgaacccatc aggtcccgct cgaagtgcac gagattgcag
    19861 aacggcttcg acaggacggt gcgcagcacg acgcccacct cccgcccgtc aacctcgacc
    19921 gtgtctatcc cccaggcggg ctttcggtag cggcgcgtcg aacgctcgaa cagttcgagc
    19981 gcggccgcgg caccgcggcc aagcgctgtc cgcgaggccg ggttcagcgg attgcgccac
    20041 atcaaacgat aggcttccgc cgtggcgcgc gccggatgca gcgcagcctg attcagctcg
    20101 tagaggtggt acagcatgat cgtccctccg actcatcaac agtctaatgc tgcatcgcgg
    20161 aatcaggtgg cgaatttttg ctgcagtgca atgacagatg gccgcagggg acatgcgccg
    20221 cgcggctata ggtagcggcg caaggtttcc gccatttttt taggcggcgt cccataagga
    20281 atgtgcgtga cgaacgcgcc gtccggaccc atgaggtaaa cgagcgcgga gtggtcgatg
    20341 ctgtaatctt cgcccatgct ttcgtcttgg aacttctggt aggtcacgcg gtaggccttc
    20401 gcggccttct cgatcgcctc gggcgagccg gtgagtccga caaagttcgt gccgaaattt
    20461 tgcacatagg ccgtgacagc ctcgggcgtg tcgcgctccg gatcgagcgt gatgaaaatg
    20521 gggacaacct tgtctgcgtc cgccccgaga tcctccagcg ccgccgacat gacttgcagc
    20581 tcggccgggc aaatgtccgg gcagtgcgtg aagccgaaga acaccagcat atagcggccg
    20641 cggaaatcct tgtcggtgac cgtcttgccg tccttgccgg cgagttcgaa tggtccgccg
    20701 ataagcgcct tgccgctcgt caccacccgc gtccgttgcg catcggtgtt gagcagcatc
    20761 acggcgccga gcaatgcgcc aatgacgaag ccgccaagga tcaccagaag caaaggccgt
    20821 cgcatgctta ctccccaagc tgaagcagcg tgccgttctc gcgggcgcta ctgagaaacc
    20881 agctgaacgt ggcgtagccc gccacgagat agatcgcgtt caggccaagc gcccaccaca
    20941 gatccgccgg atcgaacgtg ccgtgcagca gaattgagcg catgccctca aagacatggg
    21001 tcggcggaag cgccaaggcg accggctgaa gccagtcagg cagaatcgag acgggatagt
    21061 agacgcaaca cagtggcagc aacaggaatg ccagcgacca ggccagttcc tccgcgccaa
    21121 gcccatagcg caaaatgacg ccggcggcga tgagcccgag cgaccagctc gtcagcacca
    21181 gtacgaaaaa gaacgccgcg agcgccaggc cgagatcgag caggttgaac ccgaagaaga
    21241 gaaatgcggc gatggaaacc ggtaccatgc cgacggagag ccggatgatg ctccacacgg
    21301 aaagcgcggc gaccagctcg aacggccgca acgggcttat cagcaaattc cccaaattgc
    21361 gcgaccacat ctcttcgatg aaggtcgtgg agaagccgat cttcgagcgg aacagaatgt
    21421 cccacagcaa gaccgagccg atcagcacac cggcggcttg cgcgtagggg ttctccgtgc
    21481 tcgccagata tttctgcaag aagccccatg agagcatctg gaggaacggc cagtagatca
    21541 gctccacgat gcggacgccg gaactgcgca acagatacat gtagcggcgg acaagcccgg
    21601 cgatgcgccg tagcgatgcg cgtgcgccgc gcggcggcag aagaaccggc tcggtcgcat
    21661 ctatgacact ggggacgctc acgacgccag actctcgggc accgcctgcg cattgcggcc
    21721 acgtacgaca tcgaggaata cgtcttccaa tgtctgccgg ccatgggctt cgatgagcgc
    21781 ctgcggcgtc tcgtcttcga tgatgcgccc ggcctgaagc aagatcacgc ggtccgcgag
    21841 ccgctcgact tcggccatgt tgtgcgaggc gagcagcagt gtcgctttgc gttcccgcgc
    21901 atagctcttc agcttgcgcc ggacccagtc tgccgtgtcg ggatcaagcg aggcagtggg
    21961 ctcatcgagc agcaaaagct caggcgcatt gagtaatgcc ttggcaagtc cgacgcgcgt
    22021 cttcggtccg gcagagagct ttccggt
//
LOCUS       DBNHVU010000106         7731 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_2047052, whole genome shotgun sequence.
ACCESSION   DBNHVU010000106 DBNHVU010000000
VERSION     DBNHVU010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7731)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7731)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7731
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_2047052"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="AC16Y7_04155"
     CDS             complement(<1..380)
                     /locus_tag="AC16Y7_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR5708607.1"
                     /translation="MKMLFAEAPAPKLNLGKKLWLLNWPFLLLVVAIALVGAGALYSV
                     AGGTFEPWASRHIVRFCIGLALLFAVAFSDIRWWLNGAYPLYLAVLLLMLLVPFIGVE
                     SGGARRWLGVGELSFQPSEMMKITL"
     gene            complement(377..2284)
                     /gene="mrdA"
                     /locus_tag="AC16Y7_04160"
     CDS             complement(377..2284)
                     /gene="mrdA"
                     /locus_tag="AC16Y7_04160"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR5708608.1"
                     /translation="MRKWSKRAKALHYGAKKKPDYTRKQSYRFSRRALLVGAGQAGLF
                     GLLGWRLHQLQIRDSSEYRLLSDENRIMMQLVAPARGAIYDRFGTVIAEDKENLRLLV
                     LPAFCKSLPGTLDALSRVVRVSSATRSRVMRAARRQSGYYPVLVAEGLTWRQFALLNV
                     LAPQIPGMRMDRAAYRQYHHGRPMGHVVGYVGMAGKAEVDVDPVMRLPGFRTGRAGVE
                     KSFDPELRGSPGNIKYEVDVHGRVVRELDSKPSTKGRDLVLTIDHHLQTIALDRIKEH
                     RVASVVLMDVLTGGVLAMASTPSFDPNAIAFNPDPKEWRNLARGKDNPMQNRAVRGQY
                     PPGSTYKVITALAGLSAGVIDTKETMRCPGSYAVGRKRFRCWKAHGGGINVHEAIKQS
                     CDVFFYETAKRMGIDYLASVSRHFGLGRTLDCGLAGEKPGLIPDVAWKRRALFDAWYT
                     GETVISGIGQGFVLTTPLQLAVMTARVATGRAVLPKLVMSEEDQFEPWPLLEVDPAHL
                     EVVRNGMIGVVNEPRGTARRSALQIPGVQMAGKTGTSQVVSSRNEHKLSPYERETHAL
                     FVAFAPADKPRYAAVCVVEHGGGGSRAAAPVVRDVMTEALLRDPASRPAFVANNAHIV
                     PAEMASAEGAP"
     gene            complement(2281..2790)
                     /locus_tag="AC16Y7_04165"
     CDS             complement(2281..2790)
                     /locus_tag="AC16Y7_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708609.1"
                     /translation="MTKFLPALSVLFAVVATAVPWGLPADATFILPLIVVMMVFCWRA
                     IPGAGLHPAGAVGLGLLADLMSGGPLGYWALMCLIGATVGGRAGSLGETRDLGRHWLI
                     WSVLVTGLAGFGWLLASLYYFRWIDWWPIAFGAIVSVVLFPVVLYGLLWVRHGRLGHE
                     RNAIFRGGA"
     gene            complement(2787..3662)
                     /gene="mreC"
                     /locus_tag="AC16Y7_04170"
     CDS             complement(2787..3662)
                     /gene="mreC"
                     /locus_tag="AC16Y7_04170"
                     /inference="COORDINATES: protein motif:HMM:NF016011.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR5708610.1"
                     /translation="MLSRIGHGAISDARDGLVDLSAPLLEMASLPAIEARRGMDRVRL
                     YLAAFDEVDRLKKENEELKQWEWRTKLLERKVAHLRSLLNAVEEPALVYATGRVIADA
                     RGPFVRSALINLGRNDGLRIGYAVINGDGLVGRTVDAGDSVARVLLLNDLNSRIPVLA
                     GPAGVRGLALGDNSSELQLDFIPEGTAIYPGDEVYTSGSDGVLPRGLRVGVVSGTAGA
                     YKVRPYAELNSLDAVSVLFFDAPAITRADPPTDGGGGALSAAPTSGDEAKAPSASRPV
                     AAVSPPPVTQMQAGQ"
     gene            complement(3864..4901)
                     /gene="mreB"
                     /locus_tag="AC16Y7_04175"
     CDS             complement(3864..4901)
                     /gene="mreB"
                     /locus_tag="AC16Y7_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HIR5708611.1"
                     /translation="MFSRLLGSVSADMAIDLGTANTVVYVPGRGIVLDEPSVVAIEER
                     GDDQKVIAAGQEAKTMLGRTPSKIDTVQPLRDGVIADFNAAEEMIKYFIRRVNKRRLF
                     GSPRVMICVPASATSVERRAVHEAGLSAGARRVYLIEEPVAAAIGAGLPIKEPRGSMV
                     VDIGGGTTDIAVMSMGSIIYSKSIRTAGNAMDEAIVNYSRFKHHVLIGATNAEAIKKE
                     AGSAVAKANGTHVAVQLKGRDMQRGFPTEVTLGPHEIADALTLPIQQIVGGIRQALAD
                     VPPELTADICDAGIYLTGGGALLEKIDVRFQQETGVKFKIAEDPLRCVVRGTGMALER
                     LDEIADLLIKP"
     gene            complement(5209..6792)
                     /locus_tag="AC16Y7_04180"
     CDS             complement(5209..6792)
                     /locus_tag="AC16Y7_04180"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR5708612.1"
                     /translation="MTSQTKAQKGQTRDDKDRVVIFDTTLRDGEQCPGATMTFDEKLE
                     VAELLDEMGVDVIEAGFPIASQGDFESVQEISRRAENSVICGLARAQPQDIDRCAEAI
                     KPAKRGRIHTFIGTSPLHRKYQMGIEEAEVLERVTASVSRARGYTDDVEWSAMDATRT
                     EEDFLCRCVEAAIKAGATTVNIPDTVGYAYHDEFGALIRMLQERVPGADAIVFSTHCH
                     NDLGLAVANSLSGVTAGARQVECAINGLGERAGNAALEEIVMAIHTRSDVMPFWTGVD
                     TTHLTRASKLVSRVTAFPVQYNKAIVGRNAFAHESGIHQDGVLKHAGTFEIMTPESVG
                     LKESSLVMGKHSGRHAFREKLKELAYELGDNAFEDAFVRFKALADAKKHVYDEDIEAL
                     VDQEIASAHDRIKVVALTVIAGTGGPQKATITLDVDGKQQMYEAVGDGPVDATFQAIR
                     ALVPHEARLALYQVSAVTEGTDAQAEVMVRLEADGKIATGRGSDTDTMVASARAYVSA
                     LNKLLARGGRRKPETMLAS"
     gene            complement(7142..>7731)
                     /locus_tag="AC16Y7_04185"
     CDS             complement(7142..>7731)
                     /locus_tag="AC16Y7_04185"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR5708613.1"
                     /translation="RQGLRIRGVSGRDIDPDTIDWGRSDIRRYEVYQPPGPRNVLGLV
                     KFRFPNSHDVYLHDTTQKHLFANAVRAESHGCMRVQNPDQFATVLLKHDQDWTAARVS
                     SAMQSGANANKILLKQNIPVYLTYFTAVVDENGKLQQHRDLYGHDRRMVAALNGRPIP
                     AGLPDNATVSSSANYGRRTVRRRSSGNPFGGIFDF"
BASE COUNT         1450 a         2518 c         2440 g         1323 t
ORIGIN      
        1 agcgtgatct tcatcatctc cgagggctgg aagctgagtt cgccaacgcc aagccagcgc
       61 cgggcgccgc cgctctccac accgatgaac ggcaccagca gcatcagcag caagaccgcg
      121 agataaagcg gataggcccc gttcagccac caacggatgt cggaaaaggc cacggcgaaa
      181 agcagtgcca agccgatgca gaaacgcacg atatggcggc tcgcccaggg ctcgaacgtg
      241 ccgccggcca ccgaatagag cgcgcccgca cccaccagtg cgatggcgac gacaagcagg
      301 agaaacggcc agttgagcag ccaaagcttc ttgccgagat tcaacttggg cgccggggcc
      361 tcggcgaaca gcatcttcat ggtgcgccct ccgccgaagc catctcagcg ggtacgatgt
      421 gcgcgttgtt ggcaacgaag gcgggccggc ttgcgggatc gcgcaagagt gcttccgtca
      481 tcacgtcgcg caccaccggc gcggctgcgc gcgatccgcc gccgccatgc tccacgacac
      541 agactgcggc atagcgcggc ttgtcagccg gcgcgaaggc gacaaacaaa gcgtgcgtct
      601 cccgctcgta cggggacagc ttatgctcgt tgcgcgagga gaccacttgc gaggttccgg
      661 tcttgccggc catctgcacg ccgggaatct gcaacgccga gcggcgggcc gttccccgcg
      721 gctcgttgac gacgccgatc atgccattgc ggacgacctc aagatgcgcc ggatcaacct
      781 caagcaaggg ccatggctcg aactggtctt cctccgacat gacgagcttg ggcaacacgg
      841 cccggcccgt ggccacgcgc gccgtcatca cggcgagctg gagcggcgtg gtcagcacaa
      901 aaccctgacc gatcccggag atcacggtct cgcccgtata ccaggcatca aagagtgcgc
      961 ggcgcttcca ggcaacgtcg gggatcagcc cgggcttctc gccggccagt ccacaatcca
     1021 aggtccggcc aagaccgaaa tgacgcgaaa cggaggcgag atagtcgatg cccatgcgct
     1081 tggccgtctc gtagaagaag acgtcgcacg actgcttgat cgcttcatgc acattgatac
     1141 cgccgccatg ggccttccag cagcggaaac gcttgcggcc aaccgcgtag ctccccggac
     1201 agcgcatcgt ctccttcgtg tcgatcaccc cggcggaaag accagcgagt gccgtaatca
     1261 ctttgtacgt cgacccgggt ggatattgac cgcggacggc gcggttctgc atcgggttgt
     1321 ccttacccct ggccaggttg cgccactctt tcgggtcggg attgaaggcg atcgcgttgg
     1381 gatcgaagct cggcgtcgac gccatggcga gaacgccacc ggtgaggacg tccatcagga
     1441 ccaccgatgc gacgcgatgc tccttaatgc gatcgagcgc gatggtctgc aaatggtggt
     1501 cgatcgtgag aacgaggtcc ctgcccttgg tactcggctt cgagtcgagt tcgcggacga
     1561 cgcggccatg cacgtcgacc tcgtatttga tgttgccggg actgcctcgc agctcgggat
     1621 cgaaactctt ctccacgccg gcgcggccgg tgcggaaacc cggcagccgc atgaccgggt
     1681 cgacatcgac ctccgcctta ccggccatgc cgacatagcc caccacatgc cccatggggc
     1741 ggccgtgatg gtattggcgg taggcagcgc gatccatgcg catgccgggg atttgcggcg
     1801 cgagaacatt gagcagggcg aattggcgcc atgtcagtcc ttcggcgacg agcacggggt
     1861 aatagccgct ttggcggcgc gcggcgcgca tcacgcggct gcgcgtggcg gacgagacgc
     1921 gaacgaccct ggacagagca tccagcgttc ccggcaagct cttgcagaac gcgggcagta
     1981 cgaggagccg caaattctct ttatcctcgg caatgaccgt gccgaagcga tcgtagatgg
     2041 ccccgcgcgc gggcgcgacg agctgcatca tgatccggtt ttcgtcggat agtaggcggt
     2101 actcggacga gtcccggatt tgcagctggt gcaagcgcca gccgaggaga ccaaacagac
     2161 ccgcttgacc tgcgccgacg agcagcgcgc ggcgcgagaa ccggtagctc tgtttgcgcg
     2221 tgtagtcggg cttcttcttc gcaccgtagt ggagcgcctt cgccctcttg ctccactttc
     2281 tcatgcccca ccccggaaga ttgcgttccg ctcatgcccc aagcggccat ggcgaaccca
     2341 gagcaggccg taaagcacga ccggaaacag cacgacggag acgatggcgc caaaggcgat
     2401 cggccaccag tcgatccagc ggaagtaata aagtgaggcg agcagccagc cgaacccggc
     2461 gagcccggta acgagcacgc tccagatgag ccaatgacgg ccaaggtcgc gggtctcgcc
     2521 caaggagccc gcacgtccgc cgacggtggc cccgatcagg cacatcagcg cccaatagcc
     2581 cagcggtccg ccactcatga ggtctgcgag aagacctagc cccacggcac ccgccggatg
     2641 caggcctgcg cccggaatgg cgcgccagca gaacaccatc atcactacga tcagcggcag
     2701 gataaaggtt gcatcggctg gcaatcccca cggcaccgcg gtggcgacca ccgcaaataa
     2761 gacagacaga gccgggagga acttggtcat tgccccgcct gcatctgcgt tacgggtggt
     2821 ggcgagaccg ccgcgacagg ccgcgaggcg cttggcgctt tcgcctcgtc tccgctggta
     2881 ggcgcggccg atagagcgcc cccaccgcca tcggtcggcg gatcggcacg ggttatggcc
     2941 ggcgcgtcga agaacaacac gctaaccgca tcgagcgagt tcagctcggc ataggggcgc
     3001 accttgtagg cgcccgcggt gccggagacc acgccaacgc gcaagccccg cggcaatacg
     3061 ccgtcgctgc ccgaggtgta gacttcgtcg cccgggtaga tggccgtgcc ttcgggaatg
     3121 aagtcgagct gaagttcaga actattgtcg cccaaggcca acccgcgcac gccggccgga
     3181 ccggccagaa ccggaatgcg gctgttgagg tcgttgagca ggagcacgcg ggcgacggaa
     3241 tcgcctgcgt ccaccgtacg gcccacgagg ccgtcgccgt tgatcacggc gtagccaata
     3301 cgcagaccgt cattgcggcc gagattgatc aaggcgctgc gcacgaaagg cccgcgcgca
     3361 tcggcaatga cccggcccgt ggcgtagaca agcgcgggct cctccacggc gttcaacaac
     3421 gaccgcaaat gcgcgacctt tcgctcaagc agcttggtcc gccactccca ctgcttgagc
     3481 tcctcgttct ctttcttgag ccggtcgacc tcgtcgaagg cggccaggta cagccgcacg
     3541 cgatccatgc cgcggcgcgc ttcgatggcc ggcagagatg ccatttcgag cagaggcgcg
     3601 ctgagatcga ctaggccgtc gcgagcgtcc gagatcgcgc catggccgat gcggctcagc
     3661 acgagaagaa ccgcacaaag gaaataaagg ccaaaactca ggagctcgac cggcgcccgc
     3721 caggagggcc ggtgtcgacg gaacaacggt gaaccgcgct cgcgcatatg cctccaccca
     3781 ggagcgcctc gatagcgctc cactccctac tcgctttggg ccgcttgatg cgcgcccttc
     3841 ttgttgcccc gctaccctta aaactagggt ttgattagta gatcggcgat ctcatccaac
     3901 cgttccaaag ccatgccggt tccccggacc acgcagcgca gcgggtcctc ggcgatcttg
     3961 aactttacac ccgtctcctg ctggaaacgc acatcgatct tctcaagaag cgcgccgccg
     4021 ccggtgagat agatcccggc atcgcagata tctgcggtca attcgggcgg cacatccgcg
     4081 agggcctgac gaatgccacc gacaatctgt tggatcggca atgttagtgc gtcggcgatc
     4141 tcatgcggcc ccagcgtcac ttccgtgggg aagccgcgtt gcatgtcgcg gcccttgagc
     4201 tgaacggcga cgtgggtgcc gttggccttg gcgacggcgg agcctgcttc ttttttgatg
     4261 gcctcggcat tggtggcgcc aatcagcaca tggtgcttga agcgggagta gttgacgatc
     4321 gcctcgtcca tggcgtttcc ggccgtgcgg atcgacttcg aatagatgat gctccccatg
     4381 gacatcacgg cgatgtccgt ggtgccgccg ccgatgtcca cgaccatgga cccgcgcggt
     4441 tccttgatgg ggagacctgc gccaatggcc gcagccaccg gttcctcgat gagatagacg
     4501 cggcgcgcgc cagccgacag gccggcctcg tggaccgcgc ggcgctcaac cgacgtggcg
     4561 ctcgccggca cgcagatcat gacccgcggg ctgccgaata accggcgctt gttaacgcgg
     4621 cgaatgaaat atttgatcat ttcttcagcc gcgttgaaat ccgcgatgac gccatcgcgc
     4681 aacggctgaa cggtatcgat cttgctcggc gtgcgcccga gcatcgtttt ggcctcttga
     4741 ccggcggcga tcaccttctg gtcgtcgccg cgctcctcaa tggcaaccac ggatggttcg
     4801 tccagaacga tcccgcgccc cgggacatat acgacggtgt tcgccgtgcc gaggtcgatg
     4861 gccatgtctg ccgagacaga ccccaagagc cgcgagaaca tgcacccgcc tcacaatcgg
     4921 ctcgccgcag aagcggtgag cccttaacgc taccgatact gcgcacggca gaaaatatgg
     4981 ccgatcaaag ggtttcagcc gccgccaggc gcaaagccat gttgcgtcaa atcgcgatgg
     5041 cgagcaatgc gtgatgcgcc ggaatcgcaa aaaactcttc gcttgacgcg ttcaactctt
     5101 gtgggacggc accggttgcc ggggtgcacc cggccaaccg ttgcgtcctt gccacatttt
     5161 ccgcagatgc ccgcgacagg agcatgaagg ccacgcaaat aaggcgcctc aggaggcgag
     5221 catggtctcc ggcttgcgcc ggcccccgcg cgccaggagt ttgttcagcg cactgacata
     5281 ggctctggcg gaagcgacca tcgtgtcggt gtcggagcca cggcccgtgg caattttgcc
     5341 gtcggcctcc aggcgcacca tcacctcggc ctgggcgtcc gtgccttccg tcaccgcgct
     5401 cacctgatag agcgccagcc gcgcctcatg gggcaccagc gcgcgaatgg cctggaacgt
     5461 ggcgtcaacg ggaccgtccc cgacggcctc gtacatttgc tgcttgccat ccacatcgag
     5521 cgtgatggtc gccttttggg gcccgcccgt gccggcgatc acggtcagcg ccacgacctt
     5581 gatccggtca tgggccgagg cgatctcctg atcgaccagc gcctcgatgt cctcgtcgta
     5641 gacgtgcttc ttcgcgtcgg ccaaagcctt gaagcgcacg aaggcgtcct caaaagcgtt
     5701 gtcgccgagc tcgtaggcca gctccttcag cttctcgcgg aacgcatggc gcccggagtg
     5761 cttgcccatc accagcgagg actccttcag gccaacgctc tccggcgtca tgatctcaaa
     5821 cgtgccggca tgtttcagaa cgccgtcctg atgaatgccg ctttcatggg caaaggcgtt
     5881 ccggccgaca atcgccttgt tgtactggac ggggaaggcc gtcacgcggg agacgagctt
     5941 ggaggcgcgc gtcaagtggg tggtgtcgac gccggtccag aacggcatga cgtccgagcg
     6001 cgtgtggatc gccatgacga tctcttccag cgccgcattg ccggcccgct cgccgagccc
     6061 gttgatggcg cattccactt ggcgggcgcc cgccgtcacg cccgacaggg agttcgcgac
     6121 cgccaggccc aaatcgttgt ggcagtgggt cgagaacacg atcgcatcgg cgcccggcac
     6181 ccgctcctga agcatgcgga tcagcgcgcc gaactcatcg tgatacgcat agccaaccgt
     6241 gtcgggaata ttcacggtgg tcgcgccggc cttgatggcg gcctcgacgc aacggcacag
     6301 gaaatcttcc tcggtgcggg ttgcgtccat ggcggaccac tcgacatcgt ccgtgtagcc
     6361 gcgggcgcgg gagaccgatg cggtgacccg ctccagcacc tctgcctcct cgatgcccat
     6421 ctggtatttg cgatgcagcg gcgaggtgcc gatgaaggtg tggatgcggc cacgcttggc
     6481 gggcttgata gcctcggcgc agcgatcgat gtcctggggc tgggcacgcg ccaatccgca
     6541 gatcacggaa ttctccgcgc ggcgcgagat ctcctggacg ctctcgaaat cgccttggct
     6601 ggcgatcggg aagcccgcct cgatcacgtc cacgcccatt tcgtcgagaa gctcagcgac
     6661 ctccagcttc tcgtcaaatg tcatggtggc gccggggcat tgttccccgt cgcgcaaagt
     6721 cgtgtcgaaa atgaccacgc ggtccttgtc gtcacgcgtt tgccccttct gggccttggt
     6781 ctgcgaagtc atggtgctcg tccttaagcc attggcctca gcctaacacg actggcgagg
     6841 cgcttgaatc ctggagggtt tttcttgcga tcccctgaac acccgccctg acgctaagcc
     6901 aagaccgccg gagctcaggg gcgtctaagg aggagcttga gcgcgcgcgc gcgagcacgg
     6961 ccggacggaa atccgccggt tggagccgtg ttcagtgtgg tcttgcgctg catcgtcacc
     7021 ctatttccgg cgagggcttc aggcctcgcg atggctggaa aatagtgaga accgacccga
     7081 agagcaaggg gcagccccag tctcggcctg gaagaacgag gacttgcccg cgatgcgtgg
     7141 tctagaagtc gaagatgccg ccaaacgggt tgccgctgga ccgccgccga accgtgcggc
     7201 gcccgtagtt ggcggaggag gagaccgtcg cattgtccgg cagacccgca ggaatcggcc
     7261 ttccattcaa cgcggcaacc atccgccggt catggccata cagatcgcgg tgctgctgga
     7321 gcttgccgtt ctcgtccacg accgcggtga agtaggtcag atacacggga atgttctgct
     7381 tcaggaggat cttgttggcg ttggcaccgc tctgcatggc ggaggagacc cgcgcggccg
     7441 tccagtcctg atcgtgtttc agcaaaacgg tcgcgaactg atcggggttc tgcacgcgca
     7501 tgcagccatg gctctcggcg cggacggcat tggcgaacaa gtgcttctgc gtcgtgtcat
     7561 gcagatagac atcgtggcta ttggggaagc ggaacttgac gagccccagg acattgcgcg
     7621 gtcccggcgg ctgatagact tcgtaacggc gaatatcgga ccggccccag tcgatcgtgt
     7681 caggatcgat gtcgcggccg ctcacaccac ggatgcgcaa gccctgacgc t
//
LOCUS       DBNHVU010000107         1811 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_42726, whole genome shotgun sequence.
ACCESSION   DBNHVU010000107 DBNHVU010000000
VERSION     DBNHVU010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_42726"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="AC16Y7_04190"
     CDS             <1..537
                     /locus_tag="AC16Y7_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018063333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR5708614.1"
                     /translation="QAQNLSAYIPTNLISITDGQIYLSPTLFRRGQLPAVDVGRSVSR
                     VGGKAQLPAFRAVAGPLKLAYAQFEELEAFARFGAKLDEDTRAQLERGRRVREALKHE
                     EAAPIYVSRQIALLLAVVEGLFDTIPLEAMAAANAAVVNAVDATTEARDIAKGAPLKG
                     DRREAILRVAREVLREAT"
     gene            537..1463
                     /locus_tag="AC16Y7_04195"
     CDS             537..1463
                     /locus_tag="AC16Y7_04195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012456.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIR5708615.1"
                     /translation="MATLAELSRHIEVAEDLQSVVRTMKTISAVGIRSHELAEQAMSR
                     YLETVELGLQVVLRALEDTAPEAKLPRVGRTAVVLVGSEIGLCGAFNERLVTFALEIL
                     AATSAVPGERLVLTIGARADASWRAEAEPPTAHEEAPATVEALTRTVGDVLTRLDHWR
                     EEEDVEQLMLFHHRPGEHGGGIPVGTRLLPLDPAWLASLRARPWSSRRLPVPAGQPPA
                     LFRRLIRQLLFARVFTAIIQSRTAEHAERLAAMQAADRSIGEKLDDLHVAHRLTRQNM
                     ITSELLDLISGYEAITGTERRKTKPTANGVTP"
BASE COUNT          360 a          580 c          548 g          323 t
ORIGIN      
        1 caggcgcaga acctgtcagc ctatatcccc accaacctca tctccatcac cgatgggcag
       61 atctatctct cgccaaccct cttccggcgt ggacagcttc cggcggtgga tgttgggcgt
      121 tcagtctctc gcgtcggagg caaggcgcag ctgcccgcct ttcgcgcagt ggcggggccg
      181 ctaaagcttg cctatgctca gtttgaagaa ctcgaagcct tcgcccgctt cggtgccaag
      241 ctcgacgagg atacgcgcgc ccagttggaa aggggccgtc gtgtgcgcga ggcgctgaag
      301 cacgaggaag ctgcgccgat ctacgtctcg cggcagattg cgctcttact ggcggttgtg
      361 gaagggctct tcgacaccat acccctcgag gcgatggcag ccgcgaacgc ggcggtcgtc
      421 aacgcggtag atgccaccac agaagcacgc gacatcgcaa aaggcgcgcc gctcaagggt
      481 gaccggcgcg aagccatctt gcgcgttgcg cgtgaggtgc tccgcgaggc gacgtgatgg
      541 cgacactagc ggagctctct cgccacatcg aggtcgccga ggatctacaa tcggtcgtcc
      601 gcacgatgaa gacaatctca gccgtcggca ttcgcagcca tgaattggcc gaacaggcga
      661 tgtcccggta cctggagacg gtggaactgg gccttcaggt cgtgcttcgc gccctcgagg
      721 acacagcgcc cgaggccaag ttgcccaggg ttggacgcac cgccgtagta ctggtcggct
      781 ccgagatcgg cctgtgcggg gcatttaacg agcgactcgt gacatttgcg ctggagatcc
      841 ttgcagcgac cagcgccgtc cccggcgagc ggctcgtcct cacgatcggg gctcgtgccg
      901 acgcgagctg gcgtgcggag gccgagccgc ccaccgcaca tgaggaggcg cccgccacag
      961 tggaagcgct cacgcgcacg gtcggcgacg tgctcacccg cctcgaccac tggcgtgagg
     1021 aggaagacgt cgaacagctc atgctgtttc atcatcgtcc cggcgagcac ggcggcggaa
     1081 tacccgtggg cacccgcctc ttgcccctcg accccgcttg gctggcctcg ctgcgcgcgc
     1141 gcccttggtc gtctcgccgc ctaccggttc ccgccggaca accgccggcg cttttccgcc
     1201 gactgatccg ccagcttctt ttcgcccgcg tcttcaccgc aatcatccag tcccgcacag
     1261 ccgagcacgc cgagcgcctc gccgccatgc aggcggccga ccgcagtatt ggcgagaagc
     1321 tcgacgatct gcacgtggcg caccggctta cgcgacaaaa catgatcact tccgaactgc
     1381 ttgaccttat atcaggctat gaagccatta cggggaccga gcgcaggaag acaaaaccaa
     1441 cggctaatgg cgtaactccg tgattatcac gaggtggccg gcaatgcctc tgaagttcca
     1501 cctcggattt tcgtctcgtc gttgacaacc tgaaaattcg gaactcatcg tgcaggcctc
     1561 taacaattcc tggaaacttc gccacaatga gagtgcgggc gagcgtacaa aaacccattg
     1621 tgtgggcctg tcatggtgac agagtagtat ttccgcccgc caggcgagca aagctgagga
     1681 tgcgaacaga cctatggaaa agaaaacaaa gatcaacgtt tggtacatcg tcgcggccat
     1741 gctcgggatc atgatgttgc aaaacatgtg gtcgcagtca aaacaggtgg aagtgatccc
     1801 ctacagcgag g
//
LOCUS       DBNHVU010000108         5964 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1898904, whole genome shotgun sequence.
ACCESSION   DBNHVU010000108 DBNHVU010000000
VERSION     DBNHVU010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1898904"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="AC16Y7_04200"
     CDS             complement(<1..518)
                     /locus_tag="AC16Y7_04200"
                     /inference="COORDINATES: protein motif:HMM:NF020872.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HIR5708616.1"
                     /translation="MLTATNADTRSAWLIPSLVVAATITCALAGLAIAQEVSIKVLVN
                     DDPISDYDIAQRERFLAITTQTQPSPELKKKATDSIIDEHLQIQAGRTLGITPDEEDV
                     TKILSDMASKNNLDVAGLTTALGKAGVNVKTLKDRIRAQIVWQGVVRRKFRHQVNVGD
                     VDVDRALQESGEA"
     gene            complement(502..2910)
                     /locus_tag="AC16Y7_04205"
     CDS             complement(502..2910)
                     /locus_tag="AC16Y7_04205"
                     /inference="COORDINATES: protein motif:HMM:NF016348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HIR5708617.1"
                     /translation="MKRWATYHLLTRRAQPGFSASNGHSFTASLGMAAVAFGAVLAAV
                     LTPLDAAFAQAQTNLEQIIKPPTVDATEPMLLQADEMIYDNDNARISARGNVEIYYGN
                     YTLLADQIVYNRNANTLAAEGNVRMKDPDGAVITSDQLTLTDDFRDGFVDALKLVTKD
                     ETRIVAESASREAGNVTVFRKGWFTPCKLCKEDPSKPPTWRIRAGKITHKRDQAKMAF
                     NDARFDLFGVPVIWMPYFQTADPTVKRKSGFLMPSYSHSSELGSTVQVPYYFALSENF
                     DFTFAPMWTEKAGTLLLGDWRHRTSAGGYEVELAGVWNNSNSFLSPDGTVERVTDQDF
                     RGSIKTKGRFALDPYYSWGWDVLLETDETFRRFYNLDSRLKTDRVSQLYLEGLHDRNY
                     FSTRFYNTESLLFTDEEFSESVVYPIIDYDYIVDAPIVGGELSFNSNTMVFSSADGTD
                     SNRFIVQAQWRRQLIDGIGQVFTPFGRLRGDVYSLENPGLLELGGTEDVVIANPDADN
                     TIWRGNAVGGLEYRYPFIASTGLMTHTVEPIGQVIARPSSVGDQQEIPNEDALSLVFD
                     DTILFDIDKFSGYDRIETGTRANVGLRYTAQLPSGAYGRAVFGQSYQLGGENEFDTAF
                     YQTSGLASHRSDYVTGLYLQTSSYLSFSAQSRFNENNFDMERTDLGSRLNYGPVKAQV
                     NYARIPEDVPADIPIVPAAIVSNNDREEILGGGALAITNEWWLLGNLRYDLQANQTIS
                     DGLGVRYQDDCFRLDVTYQRSNISDQDIEPDQRFLVNFALKYLGTYSTTTSAFGGFSD
                     VDGN"
     gene            complement(2910..4019)
                     /gene="lptG"
                     /locus_tag="AC16Y7_04210"
     CDS             complement(2910..4019)
                     /gene="lptG"
                     /locus_tag="AC16Y7_04210"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HIR5708618.1"
                     /translation="MRGISAGGTLARYFSARFFAAMVAMFVVCCVLIFFVDFIEMLRR
                     AGKYSSDVPGILLAWMTLLRLPSFSELTLPFAVLIGTIAIFLMLSRSSELVVLRAAGV
                     SVWQFTLPAMAVAFVLGVVFVLVYNPVAAMARAEAERIYSAAFGRGESFLKANKGAGA
                     WLREDGVDGPSVVHALQVLNQGLELNGVTAFQYTPDHTLTERIEAKRAVLKNGRWELQ
                     EAWVSSVGQEPAFYERYLLSTYLTPTQVRDSLGTVFSISFWDLPNFIQIAERAGLPAT
                     QYRVQYHLLLSRPFLLVTMVLIAATCSLKGFRFGNVQINAIFGLAAGFGFFVFSEISR
                     NFAMSGLTSAAAAAWIPVIVAASLALTVLLYKEDG"
     gene            complement(4028..5209)
                     /gene="lptF"
                     /locus_tag="AC16Y7_04215"
     CDS             complement(4028..5209)
                     /gene="lptF"
                     /locus_tag="AC16Y7_04215"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HIR5708619.1"
                     /translation="MTLFSRYMFRQVTNAFLVMLLTLTAIVWLATALKELDLITSQGQ
                     GLTLFFQMTMLSLPSLITLIAPNAVLMAALYILDRMNGDSEVIVMTAAGAPVWRIGAP
                     LVALATMVGVVILVVNVYVNPACMRELRNLITQVRADLISQALQPGRFSSPERGLTFH
                     IRDRSPTGELLGLLMHDERDENQVMSYMAERGRVISGDKGAFLVMYDGYVHRFNADDE
                     EKAVQIVAFDQNMLDISKFSNKGKKGRSLAAKERSIGDLISPPKDDKLAQVKYGLLRA
                     ELHGRLATPLYPLVFIFVAIALMGQARTTRRGRWGLILAVFSIAIGLRVAGLVVGNMV
                     VLDAWAVTLVYGIPVAAILIAAWMAHVRMSPELRSRLNLGLKLQPKNIRFWTGRGMQT
                     E"
     gene            5496..>5964
                     /locus_tag="AC16Y7_04220"
     CDS             5496..>5964
                     /locus_tag="AC16Y7_04220"
                     /inference="COORDINATES: protein motif:HMM:NF014808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5708620.1"
                     /translation="MTDQTALSFASMSAAGDGVAVVFAEEGAKLTPAAQDLDKKTKRL
                     LTKAIQITAFKGAKGKTVDVLAPQGTKFTRIVLVGLGKPASYGHEDWLNLGGTVRGQL
                     TGREGTTANVYLEMVKSDVAPADVAAFALGAALRGYSFKKYKSKPQKTRGAASD"
BASE COUNT         1236 a         1693 c         1845 g         1190 t
ORIGIN      
        1 gcctcgccgg attcctggag tgcgcgatcc acgtcgacgt cgccaacgtt cacttggtgg
       61 cggaacttgc gccggacgac gccctgccag acgatctgag cgcgaatgcg gtccttgagc
      121 gttttgacgt tgacgccggc cttcccgagg gccgtggtca gaccggcgac gtcgagattg
      181 ttcttcgacg ccatgtcgga caggatcttg gtgacgtcct cctcgtcggg cgtgatgcca
      241 agagtacgtc cggcttgaat ctgcaggtgc tcgtcgatga tggagtccgt ggctttcttc
      301 ttcagttcgg ggctcggctg cgtctgtgtc gtgatggcaa ggaaccgctc gcgctgggcg
      361 atgtcgtagt ccgagatcgg gtcgtcgttc acaagcacct tgattgagac ctcttgcgcg
      421 atggccaagc cggcgagcgc gcaagtgatc gtcgccgcga ccaccagcga cggtatgagc
      481 cacgcggacc gcgtgtctgc gttagttgcc gtcaacatcg ctaaacccgc cgaacgctga
      541 ggtcgttgtg gagtaggtgc cgagatattt cagcgcgaag ttgactagga agcgctgatc
      601 gggctcgatg tcctgatcgc tgatgttgga acgctggtag gtcacatcga ggcggaagca
      661 gtcgtcctga tagcgtacac cgaggccgtc ggagatcgtc tggttggcct gaagatcgta
      721 gcgaagattg ccgagtagcc accattcgtt cgtgatggcg agcgcaccgc ctccaaggat
      781 ctcctcacga tcgttgttgc tgacgatagc ggccggtaca atcggaatgt ccgccggaac
      841 gtcctccgga attctcgcat agttcacttg cgcttttacc ggtccgtagt tcaatcgaga
      901 cccgaggtcg gtccgctcca tatcgaagtt attttcgttg aagcgtgact gagccgagaa
      961 gctaagatag ctcgatgttt gaaggtagag gcctgtcacg tagtcggagc ggtggctcgc
     1021 aagcccgctg gtctgataga acgccgtatc gaactcgttc tcgccgccga gttgatagct
     1081 ttgaccgaac acggcgcggc cataagcgcc cgacggtagt tgcgcggtat agcggaggcc
     1141 gacattggcg cgcgtaccgg tttcgatgcg gtcgtagccg gagaacttgt cgatgtcgaa
     1201 caggatcgta tcgtcgaaca cgaggctcaa cgcatcctcg ttcggaattt cctgctggtc
     1261 gccgacagac gagggccgcg cgataacctg accgatcggc tccacggtgt gcgtcatcag
     1321 gccggtcgag gcgatgaacg gatagcgata ttcgagaccg ccgacagcgt tgccacgcca
     1381 aatcgtgttg tccgcgtcgg ggttggcaat gacgacatcc tcggtgccgc caagctcgag
     1441 cagacccgga ttttcgagac tatagacgtc gccgcgcaag cggccgaatg gcgtgaacac
     1501 ttgaccgatg ccgtcgatta actgccttcg ccactgcgcc tggacgatga agcgatttga
     1561 gtccgtcccg tctgcgctgg agaacaccat cgtgttggag ttgaagctaa gctcgccgcc
     1621 gacaattggc gcatcgacaa tgtagtcgta gtcgatgatc gggtagacga ccgactcgga
     1681 aaactcctcg tcggtgaaca gaaggctttc ggtgttgtag aaccgggttg agaaataatt
     1741 ccggtcatgc aggccttcga ggtagagctg cgagacgcgg tccgtcttga ggcgggagtc
     1801 gagattgtag aagcgacgga acgtctcgtc ggtctcaagc agaacatccc agccccagga
     1861 gtagtaggga tcgagcgcga agcggccttt ggtcttgatg ctgccgcgga agtcttggtc
     1921 ggtcacacgc tccaccgtgc cgtctggact gaggaagcta ttgctattgt tccacacccc
     1981 ggcaagctca acttcgtagc cgccggcgga ggtacgatga cgccagtcgc ctaacaggag
     2041 cgttccggct ttctccgtcc acattggtgc gaaggtgaag tcgaaattct cggatagcgc
     2101 gaagtagtag ggtacttgga ccgtactgcc gagttcgctg gagtgactgt agctcggcat
     2161 caggaagccg gatttgcgct tcaccgtcgg gtccgcggtc tggaagtagg gcatccaaat
     2221 cacgggcacg ccgaagagat caaacctcgc gtcgttaaag gccatcttgg cttgatcgcg
     2281 cttgtgcgtg atcttgccgg cgcgaatacg ccatgttggc ggcttactcg gatcttcctt
     2341 acagagctta cacggggtga accaaccctt gcggaacaca gtcacgttgc cggcctcacg
     2401 ggaggccgac tccgcgacga tacgcgtttc gtccttcgta acgagcttga gcgcgtcgac
     2461 aaagccgtcg cggaagtcat ccgtcagagt gagctggtcg gaggtgatga ccgcgccgtc
     2521 ggggtccttc atgcggacat tgccttcggc ggccaacgtg ttggcgttgc gattgtagac
     2581 aatctggtcg gcgagcagcg tgtagttgcc gtagtagatc tcgacattgc ccctggcgct
     2641 gatgcgggcg ttgtcgttgt cgtagatcat ctcgtcggcc tggagcagca tgggctccgt
     2701 tgcgtccaca gtgggcggct tgatgatctg ctcaaggttg gtctgtgctt gggcgaacgc
     2761 cgcatcgagc ggcgtcaaaa cggcggccaa gacggcgcca aacgcgacgg cggccatgcc
     2821 gagtgaagcc gtgaacgagt gcccattaga cgcgctgaag ccgggctgtg cccggcgtgt
     2881 caaaaggtga tatgtcgccc agcgcttcat cacccgtcct ctttgtacaa gagcacagtg
     2941 agcgcgagcg acgccgcgac gatcaccgga atccacgctg ccgccgccgc ggaagtgagc
     3001 cccgacatgg caaagtttcg cgatatctcg gagaacacaa agaagccgaa gcctgcggcc
     3061 agtccgaaga tggcgttgat ctgaacgttg ccgaagcgga agcccttaag cgaacacgtt
     3121 gccgcgatga gcaccatggt gactagcagg aatggtcgcg acaagaggag gtggtactgc
     3181 acgcggtact gggttgcggg aagtcccgca cgttcggcta tctggatgaa gttcggtaaa
     3241 tcccagaacg agatcgagaa caccgtaccg aggctatcgc gtacctgtgt cggggtgaga
     3301 taggtactga gtagataacg ctcgtagaac gcgggttctt gtccaaccga gctaacccag
     3361 gcttcttgaa gttcccaacg gccgttcttc agcacggcac gtttcgcctc gatccgctca
     3421 gtcaacgtgt ggtccggcgt gtactggaac gcggtaacgc cgttcaactc gagaccttgg
     3481 ttaagaacct gaagcgcatg aacgacagac ggaccgtcta cgccgtcttc gcgaagccaa
     3541 gcgcccgcgc ccttatttgc tttcaggaac gactctcctc ggccgaacgc ggcggagtaa
     3601 atgcgttcgg cttccgcgcg tgccatcgcg gcgaccgggt tatagacaag cacgaagacg
     3661 acaccgagca cgaaggcgac ggccatggca gggagggtga actgccatac cgagacgccg
     3721 gccgcacgca acacgacaag ttcggacgag cggctgagca ttaggaagat tgcaattgtg
     3781 ccgatgagaa ctgcgaaggg gagagtgagt tcggagaagg acggcaaacg cagaagcgtc
     3841 atccacgcga gaagaatacc cggcacgtcg cttgagtact ttcctgctcg acgtagcatc
     3901 tcgatgaaat cgacgaagaa gatcagcacg cagcagacga cgaacatcgc caccatggcg
     3961 gcgaagaatc gcgctgaaaa gtagcgcgca agcgttcccc cggcactgat gccacgcatc
     4021 tgcgctatca ctccgtttgc atccctctac cggtccaaaa ccttatgttt tttggctgaa
     4081 gcttgagccc taagttcagc ctggaccgca gttctggtga cattctaacg tgcgccatcc
     4141 atgccgcaat tagaatcgcc gccaccggaa taccatacac cagggtcacg gcccacgcat
     4201 cgaggaccac catatttccg accacaagtc ccgccactcg caagccaata gcgatgctaa
     4261 aaacggctag aatcagcccc catcggccac ggcgtgtcgt gcgtgcctgg cccatcaggg
     4321 caatagccac gaagatgaaa acaagtggat atagtggtgt tgctaggcgt ccatgaagtt
     4381 ctgcgcgtaa aaggccatat ttcacctgcg ccagcttgtc atccttgggt ggggagatta
     4441 gatcgccaat agaccgttct ttggcggcaa gtgatcgtcc cttcttaccc ttgttgctga
     4501 acttggaaat atccagcata ttctggtcga aagctacgat ttgtacggcc ttttcttcgt
     4561 catccgcgtt gaagcggtgc acgtaaccat cgtacatgac gagaaaagcg cctttatcgc
     4621 cggagatgac gcggccgcgc tcggccatgt agctcatcac ctggttctcg tcccgttcgt
     4681 catgcatcag cagaccgagc agctcaccgg tggggctgcg gtcccggata tggaaggtga
     4741 gaccccgctc ggggctagaa aatcgtcctg gttgaagggc ttgcgagatc aggtcggcgc
     4801 gcacctgggt aatgaggttg cgtaattcgc gcatgcaggc cgggttgacg tagacgttca
     4861 ccaccaggat caccacgccg accatggtgg cgagggcgac caggggcgcg ccgatccgcc
     4921 acaccggggc gccggcggcg gtcatcacga tcacctcgct gtcgccgttc atgcgatcga
     4981 ggatgtagag ggccgccatg agcactgcgt tgggcgcgat caaggtgatc aggctcggca
     5041 ggctgagcat ggtcatctgg aaaaacagcg tcaggccttg gccctgcgag gtgataagat
     5101 ctaactcttt caaggcagta gccagccaga cgatggccgt cagcgtcagc aacatgacga
     5161 ggaacgcgtt cgtcacctgc cgaaacatat atcggctgaa gagcgtcatg accctatttc
     5221 cgccgtcccc cgagtggtgc cttcgccggt cccctacggc ccccggaaaa aggggccaac
     5281 cggctggcgc caaatacgat tctgaccagc ccaacatggc ggaatttcgg ccttttcgaa
     5341 tggcttgctt tcgctcaacg caggacttag gtagggcgac gtatttcctg ccgctgttgc
     5401 aaatccgcat cacctaagcg cgcgggcgcg atttgccggt gtgctttggc cggccgattt
     5461 gagcccgccg gcgcgaccaa tcgatggagt tctccgtgac cgatcagacc gccttgtcct
     5521 tcgccagcat gagtgcagcc ggcgacggtg tcgccgtggt gtttgcagag gagggggcca
     5581 agctgacgcc ggcagcgcag gatctcgaca agaagaccaa gcggctcctg accaaggcga
     5641 ttcaaatcac cgcgttcaag ggcgccaagg gtaagaccgt cgacgtgctt gcgccgcaag
     5701 gcaccaagtt cacacgcatt gtgttggtgg gtttgggcaa gcctgcatcg tacggccacg
     5761 aagactggct caatctcggc ggtacggtgc gcggtcaact taccggccgg gaagggacca
     5821 cggccaatgt ttatctggag atggtcaaaa gcgacgttgc gccggccgat gtggcggcct
     5881 ttgcgctggg ggctgcactt cgcggctaca gcttcaaaaa atacaagtcc aagccgcaga
     5941 agacgcgggg cgccgcgagc gaca
//
LOCUS       DBNHVU010000109         4983 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1007254, whole genome shotgun sequence.
ACCESSION   DBNHVU010000109 DBNHVU010000000
VERSION     DBNHVU010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1007254"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1263
                     /gene="gltA"
                     /locus_tag="AC16Y7_04225"
     CDS             1..1263
                     /gene="gltA"
                     /locus_tag="AC16Y7_04225"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006728797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0036440 - citrate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR5708621.1"
                     /translation="MGDAQHDLPVRKGTLGPDVIDIASLYQDTGCFTYDPGFTSTASC
                     ESKITYIDGDQGILLYRGYPIDQLAEHCTFLETAYLLLYGELPKKEQYAVFKQRITRH
                     TMVHEQMNRFYTGFRRDAHPMAIMVGVIGALSAFYHDSIDIRDARQRDMASVRMIAKM
                     PTIAAMAYKYSVGQPFVYPKNDLDYSTNFLRMCFAVPCEEYQVDPVLTRAMRRIFILH
                     ADHEQNASTSTVRLAGSSGANPFACISAGIACLWGPAHGGANEAALTMLQEIGSVDRI
                     PEFIARAKDKADPFRLMGFGHRVYKNYDPRAKIMQKTCHEVLEAVGHKDDPILKVALE
                     LEKIALEDDYFIQKKLYPNIDFYSGITLKALGFPTDMFTVLFAIARTVGWIAQWMEMI
                     EDPLQRIGRPRQLYTGATERAYVPMADR"
     gene            complement(1279..2181)
                     /locus_tag="AC16Y7_04230"
     CDS             complement(1279..2181)
                     /locus_tag="AC16Y7_04230"
                     /inference="COORDINATES: protein motif:HMM:NF017989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxI family protein"
                     /protein_id="HIR5708622.1"
                     /translation="MSQRRAAKSTAPFAPRAAKITGPLGIVAGRGRFPAVIAEAMTAH
                     DIPLHIIGLRGEASEAIERFPHTWISWGQVGKLFGALKDHGCTDIVIIGGVNRPDFDK
                     LRIDFGAVKTLPFLLSLAKGGDDHVLSRIVRFFEDKGYRVHGAGEVLPDLLAPVGTLT
                     AKAPSAGDQADIAAALKVVRALGRLDIGQAAVVSRGHVLAVEAAEGTDAMLRRCAEIR
                     RLGRTHGTRQGVLVKAPKPGQEERIDMPTIGAETIRRAAAAGLAGVAVAAGRVLMAER
                     DDAIAAANADKLFLVGEHWDDADD"
     gene            complement(2159..2965)
                     /gene="lpxA"
                     /locus_tag="AC16Y7_04235"
     CDS             complement(2159..2965)
                     /gene="lpxA"
                     /locus_tag="AC16Y7_04235"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HIR5708623.1"
                     /translation="MTNIHATAQVEPSAKLGADVKIGPFCSIGPDVELGDGVIVHSHV
                     AIDGKTTIGSSCKIFPFASIGQAPQDIKYDDEPSTVTIGPNTLVREHVTIHRGTPTGH
                     MATQIGANCFLMIGAHIAHDCNIGDNVTLVNGVTLGGHVSIGDNAIIGGLSAVHQFVR
                     IGSYAFVGGMSGIAADLIPYGMAVGNRANLNGLNIVGLKRKRFPREQIHELRQAYRML
                     FSAEGTLRERLDDVDALFSKNPLVAQIITFMKSDTDRSFCVPNNVATQSR"
     gene            complement(2978..3460)
                     /gene="fabZ"
                     /locus_tag="AC16Y7_04240"
     CDS             complement(2978..3460)
                     /gene="fabZ"
                     /locus_tag="AC16Y7_04240"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018325212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIR5708624.1"
                     /translation="MNESGVTPEQDRLETADIMKLLPHRYPFLLVDRIIDMDGDRSAT
                     GIKNVTANEPYFQGHFPGNPVMPGVLLIEGMAQTAGALCMRFLDEFEEPPLVYFMAID
                     KARFRRPVVPGDTVHYLVQKMRNRGRVWRFRARAVVDGNTVAEAEVSAVIIDSIDAET
                     "
     gene            complement(3486..4577)
                     /gene="lpxD"
                     /locus_tag="AC16Y7_04245"
     CDS             complement(3486..4577)
                     /gene="lpxD"
                     /locus_tag="AC16Y7_04245"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIR5708625.1"
                     /translation="MKHPGFFERAGPFSLRAVADATGSEIGGSADPNLKLKNVLPLDD
                     AGEGDLSFTDNPKYLPLLAETAASACIVAPKYVRRVPKETASLVMADPYRGFAKALTL
                     FYPDSLRSKTASAWLKDTATTDRLCHIHPSAVLEPDVVIEPGAIVGPEAHIGRGTTIA
                     ASAVIGYRVHIGRDCYVGPNASVTHALIGNRVGLHAGVAVGQDGFGFAMGKQGHAKVP
                     QIGRVVIQDDVEIGANSTVDRGALRDTIIGEGTKIDNLVQIGHNVVIGRHCIIVAQTG
                     ISGSSELGDLVALGGQVGVIGHVKIGAGAQIAATSNVRGDVPAGARWGGTPAKPVRLW
                     FRELTVLRQLAENKDVKVNEGEGGEGGTS"
     gene            complement(4645..>4983)
                     /locus_tag="AC16Y7_04250"
     CDS             complement(4645..>4983)
                     /locus_tag="AC16Y7_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR5708626.1"
                     /translation="PNCSRDALGGQYFWSTTAELRFPFPLIPDSLGMQGAIFVDAGSL
                     WNTSPRAVDAVTDEGSFVLDTADVRLSTGFSVIWQSPLGPLRADIAEALLKADFDETE
                     LFRFGASTNF"
BASE COUNT          949 a         1478 c         1563 g          993 t
ORIGIN      
        1 ctgggcgatg cgcagcatga cctgccggtg cggaagggta cgctcgggcc cgacgtgatc
       61 gatatcgcgt cgctttacca ggacaccggc tgcttcacct acgacccggg ctttacgtcg
      121 acggcaagct gcgagtcgaa gatcacctat atcgatggtg accagggcat attgctgtat
      181 cggggttacc ccatcgatca gctggccgag cactgcacct tcctggaaac agcctatctg
      241 ctgctctacg gcgaattgcc gaagaaagaa cagtacgcgg tgttcaagca gcggatcact
      301 cggcacacca tggtgcacga gcagatgaac cggttctaca ccgggttccg gcgtgacgcg
      361 catcccatgg caatcatggt cggcgtgatt ggggcactct cggcgttcta tcacgactcc
      421 atcgacattc gggacgcgcg ccaacgggac atggcgagcg ttcgcatgat cgccaagatg
      481 ccgaccatcg ccgcgatggc ctacaaatac tccgtcggcc agcccttcgt gtatccgaag
      541 aacgaccttg attactcgac caacttcttg cggatgtgct ttgccgttcc gtgtgaggag
      601 taccaggtcg atcctgtact gactcgcgcc atgcggcgga tcttcattct gcacgccgat
      661 cacgagcaga acgcctcgac gtcgaccgtg cggttggcag ggtcatcagg cgcgaacccg
      721 ttcgcctgta tttccgcagg cattgcgtgt ctgtggggcc cggcccatgg cggcgccaac
      781 gaggcggccc tgaccatgct tcaggagatc ggttcggtgg accgcattcc ggaattcatc
      841 gcgcgcgcga aggacaaggc cgatcccttc cgcctcatgg gcttcggaca ccgggtctat
      901 aagaactacg atccgcgggc gaagatcatg cagaagacct gccacgaggt tctcgaggcg
      961 gttggccaca aggacgaccc gatcctcaaa gtggcgcttg agctggaaaa gatcgcgctt
     1021 gaagacgact acttcatcca gaagaagctc tatccgaata tcgacttcta ttccggcatt
     1081 acgctgaagg cgctcggctt cccgaccgac atgtttacgg tgctgttcgc cattgcccgg
     1141 accgtgggct ggattgcgca gtggatggag atgatcgaag atcccctgca gcggattggc
     1201 cggccgcggc agctttatac cggcgcgacg gagcgggctt acgtgccgat ggcggaccgc
     1261 tagaggcgcg agccaggctt agtcgtccgc gtcgtcccag tgttcaccga caagaaagag
     1321 tttgtccgcg tttgctgcgg caatcgcatc gtcccgttcg gccattagga cgcgcccggc
     1381 tgcaacggca acgccggcaa gccctgcggc ggccgcgcgg cgtatggttt cggcgccgat
     1441 ggtcggcatg tcgatgcgct cctcctgccc gggtttcggc gccttgacga gcacgccctg
     1501 tcgtgttccg tgggtgcggc ctagcctgcg aatttcagcg cagcgccgga gcatggcgtc
     1561 ggtgccctcc gccgcctcaa cggctaagac gtgcccgcgt gacacgacag cggcctggcc
     1621 aatgtcaagt cgtcccaatg ctcgaaccac tttcagcgcg gcggcaatgt ccgcctggtc
     1681 gccggccgac ggggcctttg ccgtcagcgt tccgacaggc gcgagaagat cagggagaac
     1741 ctcgccggcg ccatggacgc gatagccttt gtcttcgaag aagcgaacga tgcgcgacag
     1801 cacatggtcg tcgccgccct tggcgaggct cagcaggaaa ggaagtgttt tgactgcgcc
     1861 aaagtcgatc cgcagtttgt cgaagtcagg ccggttgacg ccgccgataa tgacgatgtc
     1921 ggtacatccg tggtccttta gtgcgccgaa gagtttgccg acctggcccc aactgatcca
     1981 ggtgtggggg aaacgctcga tggcttcgga agcctctcca cggagaccaa taatgtgcag
     2041 cgggatatcg tgcgcggtca tcgcctcggc gatgacggca gggaaccgcc ctcgtcctgc
     2101 aacaatgccg agcgggccgg taatcttggc ggctcgcgga gcgaacggcg ctgtactttt
     2161 agcggctctg cgttgcgaca ttgttgggta cgcaaaaaga ccggtcggta tcgcttttca
     2221 taaacgtgat gatttgggcg acaagcgggt tcttggagaa cagcgcgtcc acgtcatcga
     2281 gtcgttccct cagcgtgcct tctgccgaga acagcatccg gtaggcctgg cgcagctcat
     2341 ggatctgctc gcgcggaaag cgtttgcgct tcagtccgac gatattcaac ccgttgagat
     2401 tggcgcgatt gccgactgcc atgccatagg gaatgaggtc ggcggcaata cccgacatac
     2461 cgccaacgaa ggcataggag ccgatgcgga cgaattggtg taccgccgat agcccgccaa
     2521 tgatcgcgtt gtcgcctatc gaaacgtgac cgcccaaggt gacgccgttt actagtgtga
     2581 cgttgtcgcc gatgttgcag tcatgggcga tgtgtgcgcc gatcatgagg aagcaattgg
     2641 cgccgatctg ggtcgccatg tggccggtcg gcgttccgcg atggatcgtg acatgttcgc
     2701 gaacgagcgt gttgggccct atggttacgg tgctcggctc atcgtcgtat ttaatgtctt
     2761 ggggcgcctg tccgatcgag gcgaagggaa agattttgca gctcgaaccg atagtcgtct
     2821 tgccgtcaat ggcgacgtga gagtgaacga tcacgccgtc gccaagttca acgtcgggtc
     2881 caattgagca gaacggccca atcttaacat cggcgccaag tttggcgctc ggctcgacct
     2941 gcgcggtcgc gtggatattg gtcatgtatt tttccggcta ggtctcggca tcgatcgaat
     3001 cgatgatcac agcgctgacc tcagcctcgg caacggtgtt gccgtcgacg acagcccggg
     3061 cgcggaagcg ccaaacgcgg ccacgattac gcatcttctg aacgagataa tgaacggtgt
     3121 ctccaggaac gacagggcgg cggaagcgcg ctttgtcgat ggccatgaag taaacgagtg
     3181 gcggttcttc gaactcgtcg aggaagcgca tgcatagcgc gcctgcagtt tgcgccatgc
     3241 cttcaataag gagcacgccg ggcatgaccg gattgccagg gaaatggccc tggaaatagg
     3301 gttcattcgc cgtgacgttc ttgatgccgg tcgccgaccg gtcgccatcc atgtcgatga
     3361 tgcgatcgac caataagaat ggatagcgat gcggcagcag cttcatgatg tcggctgttt
     3421 ccagccgatc ctgctccggt gttaccccgc tctcgttcat tcgggccggc tcctttgatg
     3481 gggactcatg acgttccgcc ctccccgcct tcgccttcgt tgaccttcac gtccttgttc
     3541 tcagcgagct gccgcaggac ggtcagctcc cggaaccaga gcctcactgg cttggcgggc
     3601 gtgccgcccc aacgtgcacc ggcgggcaca tcgccccgaa cattgctggt tgcggcgatt
     3661 tgggcgccag caccgatctt gacgtggccg atgacgccaa cttggccgcc aagagcaacg
     3721 agatcgccga gttcggaact gcccgagata cctgtttggg ccacgataat acagtgtctt
     3781 ccaatgacga cgttgtgccc gatttgcacg agattatcaa ttttcgtgcc ttcgccgatt
     3841 atggtgtccc gcagcgcgcc gcgatcgacg gttgaatttg cgccaatttc gacgtcgtcc
     3901 tggatcacca cccggccgat ctgaggcact ttggcgtgcc cctgctttcc catggcaaat
     3961 ccgaaaccgt cctggccgac ggcaacgcct gcgtggaggc cgacccgatt gccgatgagc
     4021 gcgtgggtca cggacgcgtt gggcccaaca tagcagtccc ggccaatatg gacccgataa
     4081 ccaatgacgg cactggccgc gatcgtggtg ccgcggccga tatgcgcttc cggcccgaca
     4141 atggcgcctg gttcgatgac gacgtccggc tcgagcaccg cactcggatg gatgtggcac
     4201 aggcgatccg tggtggctgt gtctttgagc caggcgcttg ccgtcttcga cctcagtgag
     4261 tccggataga acagcgtcag tgctttggcg aagccccgat aagggtcggc catgacgagg
     4321 ctggcggttt ccttcgggac cctgcgaacg tatttcgggg ctacgatgca cgcacttgcc
     4381 gcggtctcgg ccagcagagg cagatatttc ggattgtccg tgaaggacag gtcgccctcg
     4441 cctgcatcat ccaggggcag gacgttcttg agcttgagat tgggatcggc ggatccgccg
     4501 atctcggagc cggtggcgtc ggcgacggcg cgcaaggaga acggaccggc ccgctcgaaa
     4561 aaacccgggt gtttcatcaa atctcccgcg agtggctaag cgcgttgaac catggcgcgt
     4621 agccataaac cgaactggcg tcttttagaa gttcgttgag gcgccgaagc ggaaaagttc
     4681 agtttcgtcg aaatcggcct tgagaagcgc ctcggcgatg tcggcgcgga gcggcccaag
     4741 gggggactgc caaataacgc tgaagccggt cgaaagacgg acatcggccg tgtcgaggac
     4801 gaagctgccc tcgtccgtca ccgcatccac ggcgcgcggg ctggtgttcc agagcgaacc
     4861 tgcatcgacg aagatggcac cctgcattcc aaggctgtcc gggatcagcg ggaacgggaa
     4921 acggagttcg gccgtggtgg accagaagta ctggccgcct agagcgtcgc ggctacaatt
     4981 cgg
//
LOCUS       DBNHVU010000110         6165 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1527345, whole genome shotgun sequence.
ACCESSION   DBNHVU010000110 DBNHVU010000000
VERSION     DBNHVU010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6165)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6165)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6165
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1527345"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="AC16Y7_04255"
     CDS             <1..909
                     /locus_tag="AC16Y7_04255"
                     /inference="COORDINATES: protein motif:HMM:NF013625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HIR5708627.1"
                     /translation="IGGKVAMMTGKMVAPAPIEHPTLFEANSASVASPPAGSVAAGAK
                     EAARAGAGIPADTAKASSDANAPLHILVTRREERDIAIGVQKILASMGYLAPQDKFVG
                     YLGDGTKKAIKAFEKEHGLRQRGRFNEAIAAKIYEAAGLGPLPDAYLFLRKGFSRIGS
                     VPVTIRDPRRNLGTHLFTYMRPSDGSEPQWVGISLEGDKSSKVLDRITIPDSMRGEIS
                     RSLTSGATFIVANVAKHSSVLPEGDDFIVRTNDSPESNSKPEKVVAKPRKKRVVEQTL
                     VPARSLRARRKVHGPRGFGMFSRRHR"
     gene            complement(906..2246)
                     /locus_tag="AC16Y7_04260"
     CDS             complement(906..2246)
                     /locus_tag="AC16Y7_04260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5708628.1"
                     /translation="MTDPTPEPLTLRDLAAGPARNASVSFKPFWWEAAPRRQTPGRTI
                     PARTDVAIVGSGYTGLSAALTLLAKGRDVVVLDRGVPGHGASTRNGGQIGSGNQKFPV
                     KRLVALHGEKKAAELLREGTAMLDYIAHLIQAENIDCHFQRCGRFRGAMRPEHYEAMA
                     RDMEDLKRVAGVESFMVPRTEQHNEIGTDVFFGGSVLPKDASLHPALFHSGLMQCVED
                     LGGVIVGNAGVHHIARARSGFRVDTDAGPLQARDVLVATNGYTDRLSRTVHRRIVTIR
                     SGLIATAPISPETMARLMPKSRVYGNTNRVFYYFRAAPDESRLVWGGRAGKAAGLYAY
                     RHLAKNLLSVFPHLGATDVTHAWDGQIGYTYDELPHLGRMADGVHYTVGYCGTGVSRA
                     TYFGHKIALQMVGDPEGRTAFDSLTFPAFPFRAIAQAAVPIVEAWYRFRDSTNL"
     gene            complement(2243..3781)
                     /locus_tag="AC16Y7_04265"
     CDS             complement(2243..3781)
                     /locus_tag="AC16Y7_04265"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HIR5708629.1"
                     /translation="MQAIFDHGPLAPCPAPFNLAAYVLARAGTLGLSPALAVISPDGA
                     ETWSYARLEAAVLGAARGLQAEGLQAGDRLLMRLGNSVDFPICYLAALAADLVPVPTS
                     SLLTAPEVTQIAEDVAPSLIVAEDGLALPAGAPCPVLSVERLRAMYALAPVTYVMGDP
                     DRPGYIIYTSGTSGQPRAVTHAHRAIWARQMMWDYWYGLTEADRLLHAGAFNWTYTLG
                     TGLLDPWTRGATALIPAAGVTPDELPHLLQQHKATIFAAAPGVYRQLLRTPPPPLPDL
                     RHGLSAGEKLPDATRAAWEAATATPVFEAFGMSECSTFVSGSPAHPAPFGTLGYPQEG
                     RRVAVLNEDGPVGIGEPGVLSIASRDPGLMLGYWNAPQETAQRFQGEWFLTGDLVSMG
                     TDGALTYLGRADDMMNAGGIRVSPLEVEDALNAHPAILESAATEVTVKADTTVIAAFY
                     VANDTVTPDELDGFVSGRLARYKVPRIYGQVDALPKGPNGKLLRRRLRQQHEDTLPGS
                     PHQK"
     gene            3929..5431
                     /locus_tag="AC16Y7_04270"
     CDS             3929..5431
                     /locus_tag="AC16Y7_04270"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HIR5708630.1"
                     /translation="MTDLDTLSHVTMEGLGFLSTLFVFVIGLAMLAIAIVFIVDVTQT
                     KDAVRRNYPVIGRFRYVFSKLGEFFRQYFFAMDREEMPFNRAEREWVYTSAQGGDNTV
                     AFGSTKNLTPVGTVIFINCPFPTLTEDSVPTPPITIGPYCDNPYETESIINISGMSFG
                     AISQPAVRALSNGAKLAGCWYNTGEGGLSPYHLDGGCDIVYQIGTAKYGVRTSDGGLD
                     EAKLRAIAERPEVKMFELKMSQGAKPGKGGILPAVKVTPEIAEIRGIPVQTASISPNG
                     HPEIRNVAQLLDMIERIRKVTNKPVGFKAVVGAYGWLDNLCKEIQARGIETAPDFITV
                     DSGDGGTGAAPMPLMDNVGLPVKEALPMVADILTRNGLRDRVKLIASGKLVTPAEVAW
                     AYCAGADVAVSARGFMFALGCIQALKCNKNTCPTGITTHDPRLQRGLDPEDKAERVRN
                     FVERIRYDVGVIAHSCGVPHPRALKRYHCRIVQNSGRSIPLDELYPPVAA"
     gene            complement(5428..>6165)
                     /locus_tag="AC16Y7_04275"
     CDS             complement(5428..>6165)
                     /locus_tag="AC16Y7_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015823060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HIR5708631.1"
                     /translation="NMGWMHDTLGYMEKEPVHRQHHLDQLTFGLTYAFTENFVLPLSH
                     DEMVHGKGSLIGKMPGDRVHKFANLRAYFGFMWGHPGKNLLFMGGEFAQEAEWNHDRS
                     LDWHLLEDDMHRGVQALVRDLNRLYRDTPALHVLDTDPKGFEWVDASDKEACVISFLR
                     WGRDGDAPALIVCNFSPVPRENYRIGVPQGGRWIERLNTESSNYGGSGEGNGGAVDAE
                     EVSWHGRDRSLALTLPPLSTLIFVADV"
BASE COUNT         1125 a         1958 c         2004 g         1078 t
ORIGIN      
        1 atcggcggga aagtcgcgat gatgacgggc aagatggttg cgcccgcgcc gattgagcat
       61 ccgacgctct tcgaggcgaa ttccgcgtcc gtcgcttcgc cgccggcggg cagcgttgcg
      121 gccggtgcca aggaagccgc gcgcgccggc gccggaattc ctgccgacac tgcaaaggcg
      181 tcgtccgatg cgaacgctcc cttgcatatt ctcgtcacgc gccgtgagga gagggacatc
      241 gccattggcg ttcagaagat tctcgcctcg atgggctatc tcgcgcccca ggacaagttc
      301 gtcggttatt tgggcgacgg caccaagaag gcaatcaagg ccttcgagaa ggaacatggc
      361 cttcgccaga ggggacgctt caacgaggcc atcgcggcga agatctacga agccgcgggg
      421 cttgggccgc tccccgacgc ctatctgttt cttcgcaagg gctttagccg catcggcagt
      481 gtccccgtca ccatccgcga tccccgccgg aacctgggaa cgcatctgtt cacctatatg
      541 cgccccagcg acggatcaga gccgcaatgg gtcggcatca gtctcgaagg cgacaaatcg
      601 agcaaggttc tcgaccgaat cacgatcccg gactccatgc gcggggaaat ctccaggagc
      661 ttgacctcag gcgccacgtt catcgttgcc aatgtcgcca agcattcgtc ggtgctgccc
      721 gagggcgacg acttcattgt ccggacgaac gattccccgg agtcgaactc aaagccggag
      781 aaggttgttg ccaagcccag gaaaaaacgg gtcgtcgagc agacgctcgt ccccgcgcgc
      841 tctcttcggg caaggcggaa ggtccacgga ccccgcggct tcgggatgtt ctctcgtcgc
      901 caccgctaga gattcgtgga atcgcgaaag cggtaccagg cctcgacaat cggcacggcg
      961 gcctgcgcaa tcgcgcggaa gggaaaagcc ggaaacgtca gtgagtcaaa cgccgttcgc
     1021 ccttctggat cgcccaccat ctgcaacgcg atcttgtgcc cgaaataggt ggcgcgcgac
     1081 acgcccgtgc cgcaatagcc gaccgtgtag tgaaccccgt ccgccatgcg accgagatgc
     1141 ggaagttcgt cgtaggtata gccgatctgc ccatcccagg catgggtgac gtccgtcgcg
     1201 ccaagatggg ggaatacgct caagagattt ttggcgagat gacggtacgc gtatagccct
     1261 gcggctttcc ctgcgcgccc gccccagaca agccggctct cgtcgggcgc ggcacgaaaa
     1321 taatagaaga cgcggttggt gttgccgtag acgcgggact tgggcatcag gcgcgccatc
     1381 gtctcgggcg aaatgggtgc tgtggcgatc aagccggacc ggatagtgac gatgcggcga
     1441 tgaaccgttc gtgagagccg atccgtgtaa ccgttggtcg caaccaagac gtcgcgggct
     1501 tgcagcggac ctgcgtcggt gtccacgcga aagccgcttc tcgcgcgcgc gatgtggtgg
     1561 acgcctgcat tgccgacaat cacaccgcca aggtcctcca cgcattgcat taggcccgaa
     1621 tggaacagtg ccgggtgcag cgatgcatcc ttcggtagga cggagccgcc gaagaaaaca
     1681 tcggtgccga tttcgttgtg ctgttccgtg cgcggcacca tgaagctctc cacgccggca
     1741 acgcgtttga gatcctccat gtcgcgcgcc atggcctcgt agtgctcggg ccgcatggcg
     1801 ccccggaacc ggccgcaacg ctgaaagtgg cagtcgatgt tttcggcctg gataagatgg
     1861 gcgatgtagt cgagcatcgc cgtgccttcg cgcaagagct ctgccgcctt cttctcgccg
     1921 tgcaacgcga caaggcgctt gaccgggaac ttctgattgc cgctgccgat ctgcccgcca
     1981 ttgcgcgtgc tggcgccgtg ccccggcacg ccacggtcga gcacgaccac atcgcggcct
     2041 ttggcgagga gcgtcagcgc ggccgacagt cccgtgtagc cggacccgac aatggcgacg
     2101 tccgttctgg ccgggatcgt gcggccaggc gtctgccgcc gtggggccgc ctcccaccaa
     2161 aaaggcttga agctgacaga ggcgttgcgc gcggggcccg cggcaagatc gcgaagggta
     2221 agcggttctg gtgtggggtc cgtcatttct ggtgcggcga tcccggcaaa gtgtcttcgt
     2281 gttgctggcg caggcgccgc cgcagcagct taccatttgg gcccttcggc agcgcgtcaa
     2341 cctgcccata aatgcgcggg actttgtagc gggcaaggcg gccagagacg aagccatcca
     2401 gttcgtctgg cgttacagtg tcgttcgcca cgtagaaggc ggcgatgacc gtggtgtcgg
     2461 ccttgaccgt gacttccgtc gctgcacttt cgaggatcgc cggatgcgcg ttgagcgcgt
     2521 cttcgacctc gaggggcgag acgcggatgc cgcccgcatt catcatgtcg tcggcccggc
     2581 cgagataggt gagggcgccg tcggtcccca tgctcaccag atcgccggtg aggaaccact
     2641 cgccttggaa gcgctgcgcg gtctcttgcg gcgcgttcca atagccgagc atcaatcccg
     2701 gatcgcgcga tgcgatgctg agcacgccgg gttctccgat tccaacggga ccatcttcgt
     2761 tcagcacggc cacgcgccga ccctcttgcg gatagcccag cgtgccgaat ggtgcgggat
     2821 gcgccggtga gccggagacg aacgtggagc attccgacat gccgaaggcc tcgaagaccg
     2881 gcgtagccgt cgcggcttcc caggcagcac gggtcgcgtc cggcagcttc tcgcccgccg
     2941 aaagcccatg gcgcaagtcc ggaaggggag gcggcggggt ccgcaagagc tggcggtaga
     3001 cgcccggagc ggcggcaaag atcgtcgcct tgtgttgctg taagaggtgc ggaagttcat
     3061 cgggcgtgac gcccgccgcg gggatcagcg ccgttgcgcc gcgcgtccac ggatccaaga
     3121 gccctgtgcc gagcgtatag gtccagttga aggcgccggc atgcagcagg cggtcggcct
     3181 cggtcagccc gtaccagtag tcccacatca tctggcgggc ccaaatcgcc ctatgagcat
     3241 gggtcaccgc gcggggctgt cccgaagtcc ccgacgtata gatgatgtag ccgggacggt
     3301 cggggtcgcc catgacatag gtcacagggg caagcgcata catggcgcgt aggcgttcca
     3361 cggacagcac cgggcagggc gcgccagcgg gaagcgcgag cccatcctcg gccacgatca
     3421 ggctgggggc aacgtcctcg gcgatctgcg tgacctcggg cgcggtcagg agtgaggagg
     3481 tcgggacggg cacaaggtcg gcggccagcg cggcgagata gcagatggga aagtccacac
     3541 tgttgccaag ccgcatcagc aagcgatctc ccgcctgcaa gccctcagct tgaagcccgc
     3601 gcgcagcccc aagcacggcc gcttcaagcc gtgcatagct ccatgtctcg gcgccgtcgg
     3661 gcgagatcac cgcgagcgcg ggcgatagcc caagcgtgcc cgcgcgggcc agcacatagg
     3721 ctgctagatt gaacggggcc ggacagggcg ctagcggccc gtgatcgaag atcgcctgca
     3781 tagctcgctg ctacgcgttc gcccgcgccc tggcaagcgt cgaggcgtct gttcgtcaag
     3841 cgaacgtgat gtcttttgtg gtgacccgcg cgcgagaacg gccacagtgg cccaaagcgg
     3901 cttcacggtt tcgaaggacg gctcgatcat gaccgacttg gacacactct cccacgtgac
     3961 gatggaaggc ctgggcttcc tctccacgct cttcgtgttc gtcatcgggc tcgcgatgct
     4021 ggctatcgcc atcgtgttca tcgtcgacgt cacccagacc aaggatgccg tgcggcggaa
     4081 ctacccggtc ataggccgct tccgctacgt gttctcaaag ctgggcgagt ttttccgcca
     4141 gtatttcttc gccatggacc gggaggagat gccgttcaac cgcgccgagc gggagtgggt
     4201 ctacacctcg gcccaaggcg gcgacaacac ggtcgccttc ggctccacca agaacctgac
     4261 ccccgtcggc acggtcatct tcatcaactg cccgttcccg acgctcaccg aggactcggt
     4321 gccgacaccg cccatcacta tcgggccgta ttgcgacaac ccctatgaaa cagagtccat
     4381 catcaacatt tcgggcatga gctttggcgc catttcgcag cctgccgtgc gcgcgctctc
     4441 gaacggcgcc aagctggcgg gctgttggta caacaccggc gagggcgggc tgtcgcccta
     4501 tcatctcgat ggcggttgcg acatcgtcta ccagatcggc acggccaagt atggcgtgcg
     4561 gacctcggac ggcgggcttg acgaggcgaa gctgcgtgcg atcgcggagc ggccggaagt
     4621 gaagatgttc gagctcaaga tgagccaggg cgcgaagccc ggaaagggcg gcatcctccc
     4681 ggcggtcaag gtcacgccgg agatcgccga gatccgcggc atccccgttc aaacggcgtc
     4741 catctcgccg aacggacatc ccgagattcg caacgtcgcg caacttctcg acatgatcga
     4801 gcgcatccgg aaggtgacca acaagccggt cggcttcaag gctgtggtcg gggcctatgg
     4861 ctggctggac aatctgtgca aggaaatcca ggcgcgcggc atcgagaccg cgccggactt
     4921 catcacggtc gactcaggcg acggcggcac gggcgctgct cccatgccgc tgatggacaa
     4981 tgtcggcctg ccggtgaagg aggcgttgcc catggtcgcc gacattctca cccgcaacgg
     5041 gctgagggac cgcgtgaagt tgattgcgtc gggcaagctg gtgacgcccg cggaagtcgc
     5101 ctgggcctat tgcgcgggcg ccgacgtggc ggtttccgcg cgcggtttca tgttcgcgct
     5161 cggctgcatc caggcgctga agtgcaacaa gaacacgtgc cccaccggaa tcacgacgca
     5221 tgatccgcgc cttcaacgtg gtctagatcc cgaagacaaa gccgagcggg tcaggaattt
     5281 cgtcgagcgt attcgctacg atgttggtgt gattgcccat tcatgcggtg tgccccatcc
     5341 gcgcgcgctc aagcgctatc attgccgcat cgtgcagaac tccgggcgct caatcccgct
     5401 ggatgagctt tatccgcctg tcgccgccta aacgtctgcc acgaagatta gcgtcgaaag
     5461 cggcggcaag gtcagagcga gcgagcggtc tcgcccatgc caggaaacct cttctgcgtc
     5521 cacggcgccg ccattaccct caccggagcc gccatagttg cttgactcgg tattgaggcg
     5581 ctcgatccaa cgcccgcctt gcggcacgcc gatgcgataa ttctcgcgcg gtacgggtga
     5641 gaagttgcag acgatgagcg cgggcgcatc gccatcgcgg ccccagcgca ggaaggagat
     5701 cacgcatgcc tccttgtcgc tggcatcgac ccactcgaag cctttcgggt cggtgtcgag
     5761 cacatgcagc gcgggcgtgt cgcggtaaag ccggttgaga tcgcgcacca gcgcctgcac
     5821 gccgcgatgc atgtcgtctt cgagcaggtg ccaatcgagg ctccggtcgt ggttccactc
     5881 cgcctcttgc gcgaactcgc cgcccatgaa cagcaagttc ttgccgggat ggccccacat
     5941 gaacccgaaa taggcgcgca agttcgcgaa cttgtgcacg cggtcgccgg gcatcttgcc
     6001 gatgagcgag cctttgccgt gcaccatttc gtcatgcgag agaggcagca cgaagttctc
     6061 ggtgaaggcg taagtgaggc cgaaggtgag ctgatcgagg tgatgctggc ggtggaccgg
     6121 ctccttctcc atgtagccaa gcgtgtcgtg catccagccc atgtt
//
LOCUS       DBNHVU010000111         6752 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_859274, whole genome shotgun sequence.
ACCESSION   DBNHVU010000111 DBNHVU010000000
VERSION     DBNHVU010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6752)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_859274"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /gene="purH"
                     /locus_tag="AC16Y7_04280"
     CDS             complement(<1..1433)
                     /gene="purH"
                     /locus_tag="AC16Y7_04280"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR5708632.1"
                     /translation="METQKVSRALLSVSDKTGLVDFAKALAARGVVLVSTGGTAKALR
                     NEGLDVVDVSEVTGSPEMMDGRVKTLHPKIHGGLLAMRGNAEHEAAMAEHAIPAIDLL
                     VVNLYPFEATVRAGGNFNVCIENIDIGGAAMIRAAAKNHAGVAVVVDPKDYMDVLDEI
                     ERHDGGTTFDLRKKLAAKAYAHTGAYDGAIATWFAEQLDEDTPEKRVIAGTLERALRY
                     GENPHQWAAFYQTAEQRPGVASAVQVQGKELSYNNLNDTDAAFELVAEFDPKQSAAVA
                     IIKHANPCGVAVAPTIAQAYEKALACDGVSAFGGIVALNRDLEADAATEIVKLFTEVI
                     IAPEAGDEAKEIVAAKKNLRLLITGGLPDPRCEGLTFRSLAGGFLVQSRDNGFVDAHA
                     LKVVTKRKPTDQEFADLVFASTVAKHVKSNTIVYAKDGATVGIGAGQMSRVDAARIAA
                     RKAKDASDALGLKEPLTTGSVAASDAF"
     gene            complement(1438..3270)
                     /locus_tag="AC16Y7_04285"
     CDS             complement(1438..3270)
                     /locus_tag="AC16Y7_04285"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019552.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HIR5708633.1"
                     /translation="MLERRGRQRLETTGAGEWSMTHMAGDQAFERDSRVEEVYLGGPS
                     PLRRLMLSSMASISYPGGQAEQLLLAPQELRTADPSFANEVYNGHFGLAGKLAELGAD
                     SPFEIKPPSANWARDLYGFGWLRHLRAADSELSREQAKALVHDFIRLRRQVHGLAWQP
                     EIVARRVISWLSNSVLILDAGEPQAYESFLKALTGQLRYLSASYRDAPDGVPRLLSLM
                     GLLYAGLCIADQQAVVDRYTRPLCKELDRQILPDGGHISRIPEALIELLLDLLPLRQC
                     FVARDRTPPKELADAIDRIMPMLRFFRMGDGTMSRFNGAGPTPTDALATVLAYDDIEG
                     APVRTAANSGYVRVEAGTTLVICDLGPPPVASLTTNMHAGFLSFEMSSGDAPVIINCG
                     APSPDQEGWRMFSRTTPAHSTLSLEEDSLAEFRAGANGAEPAADATMVGPLNPQGAFS
                     DQGDNLRIKGSHDGYMGRYGVNHSRQILISPDGNLLSAEDKLTAPRGLKSPEGLIAGA
                     YAIRFHLHPSVKAQLMGDGEAVTLILRNGETWRISSNAEHMAIEDSVLLADQRGPQST
                     SQVVLTGMMGEARELRILWKIERTSDAHPQALDDPAGDAGNSGA"
     gene            complement(3330..4694)
                     /locus_tag="AC16Y7_04290"
     CDS             complement(3330..4694)
                     /locus_tag="AC16Y7_04290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HIR5708634.1"
                     /translation="MTTSNTQTNSLSRTRDHQVGLPARRAAADLLNAVLQKKQPLDEV
                     LGRARDGGYLFDLPLRDRALARAIVGTSLRHKGQIDAVLNTFLERGLPSRAGTLYAIL
                     LSGAAQLLFMSVPPHAAIDLAVRLAKWDPRAKRYDKLVNAVLRRVADKGGEIAAKLDA
                     ARVNTPDWLWDRWVRAWGEERTRASAEAALVEPPLDLTVKSDPDGWAARLGGRVLPTG
                     SVRILPKGRIEDLDGFIDGNWWVQDAAASLPAQLLGDLTGKRVADLCAAPGGKTAQLA
                     LAGAEVVAVDHSKSRLERLEENLKRLGLTVETVRADAGAWQPEERFDAILLDAPCAST
                     GTIRRHPDIPYLKSDKDIAELVDVQARLLDNAVSLLKPGGTLVYSTCSLEPEEGEAQI
                     AALMARNPAIRIDPLRKDEPLGDTEWAEASGILRTFPFQLDLGSPDWSGLDGFFAARL
                     RLAD"
     gene            complement(4798..5727)
                     /gene="htpX"
                     /locus_tag="AC16Y7_04295"
     CDS             complement(4798..5727)
                     /gene="htpX"
                     /locus_tag="AC16Y7_04295"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HIR5708635.1"
                     /translation="MSYFRTAILLAGLTALFMGIGFLMGGQTGMIIAFVIAAGMNLFS
                     YWNSDKMVLRMYGAREVDARTSPKLYDMVQQLAGNAGLPMPKVYVMENPQPNAFATGR
                     NPKNAAVAVTTGLVQSVTREELAGVIAHELAHIKHHDTLLMTITATIAGAISMLANFA
                     MFFGGSRDNNGIGILGTIALIILAPLAAGLVQMAISRTREFEADRGGAEISGQPLWLA
                     SALQKIAGAAHHIPNAEAERNPSSAHMFIINPLSGLRMDKLFSTHPATDDRVARLVAM
                     SKTMGGAHEPNLAPEPTGPWSGATARTTRGPWG"
     gene            5939..6421
                     /locus_tag="AC16Y7_04300"
     CDS             5939..6421
                     /locus_tag="AC16Y7_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012272.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin domain-containing protein"
                     /protein_id="HIR5708636.1"
                     /translation="MPAALQASQTATKVKPRRARRSTRSKDTELTAEQKRLIRVSYLK
                     VEPALELVAQLFFLKLFRLDPSLRKKFSGPVEMQARKFAAGAKLAMISLGHEDGLSPT
                     LKLLGTRHRQMGIRTRHYRTMSRALVWTLERSLEKAFDRDTKDAWTTMLAYFTRTMSA
                     "
     gene            complement(6448..>6752)
                     /locus_tag="AC16Y7_04305"
     CDS             complement(6448..>6752)
                     /locus_tag="AC16Y7_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708637.1"
                     /translation="VVGRLGTSGDPTQALALAVGNRTINVAGHFKDMTQETIADGIKI
                     VVDDHEVVVSSDQLSVDGKTQVLEPGKDVMLYVGKNGGLQVKVMRESDEAPEGASN"
BASE COUNT         1160 a         2179 c         2190 g         1223 t
ORIGIN      
        1 aagaaggcat ccgaagccgc gaccgaaccg gtcgtcagcg gttccttcag ccctagcgca
       61 tcgctcgcgt cttttgcctt gcgcgcggca atgcgcgcgg cgtccacgcg gctcatttgg
      121 cctgcgccaa ttcccactgt agcgccgtcc ttcgcgtaga cgatggtgtt cgacttgacg
      181 tgcttcgcca cggtggaggc gaagacgagg tcggcgaact cctggtcggt gggcttgcgc
      241 ttggtcacca ccttcaaagc gtgcgcatcg acgaacccgt tgtcgcggga ttgcaccaga
      301 aagccaccgg cgagcgagcg gaacgtaagg ccttcgcaac gcggatcagg cagaccgccg
      361 gtgatgagca gccgcaggtt cttcttggct gccacgatct ctttcgcctc gtcgccggct
      421 tcgggcgcga tgatcacttc ggtgaagagc ttgacgattt ccgttgccgc gtccgcctcg
      481 aggtcgcggt tgagcgcgac aatgccgccg aaggcgctga ccccgtcgca ggcgagcgct
      541 ttctcatagg cttgcgcgat cgtcggcgcg acggcaacgc cgcacgggtt ggcgtgcttg
      601 atgatggcga ccgccgccga ctgcttcgga tcgaactcgg cgacaagttc gaacgcggcg
      661 tccgtgtcat tcagattgtt gtaggagagc tccttgcctt gcacttgcac ggcggacgcc
      721 acgccggggc gttgctccgc cgtctgatag aaggcggccc attggtgcgg gttctcgccg
      781 taacgcagtg cgcgctccag cgtgcccgcg atgacgcgct tctccggcgt gtcctcatcg
      841 agttgctcgg cgaaccaagt ggcgatcgcg ccgtcatagg cgccggtatg cgcgtaggcc
      901 ttggccgcga gcttcttgcg caagtcgaac gtggtgccgc cgtcatggcg ctcgatctcg
      961 tcgagcacgt ccatgtagtc cttggggtcc accaccacgg cgacgcctgc gtggttcttt
     1021 gcagcggcgc ggatcatcgc cgccccgcca atgtcgatat tctcgatgca gacattgaag
     1081 tttccacccg cgcgcactgt ggcctcgaac ggatagagat tgaccacgag aagatcgatg
     1141 gcgggaatgg cgtgctccgc catcgccgct tcgtgctcgg cgttgccgcg catggcgagc
     1201 agcccgccat ggatcttcgg atgcagcgtc ttcacccggc cgtccatcat ctcggggctg
     1261 cccgtgacct cggaaacgtc gacgacgtcg agcccctcgt tgcgcagcgc cttcgcggtg
     1321 ccgccggtgg agacaagcac gacaccgcgc gcggcaagtg ccttggcgaa atcgacaaga
     1381 cctgtcttgt cgctgaccga gagcagcgcg cgggaaacct tttgtgtctc catgagccta
     1441 tgcgccgctg ttgccggcgt cgccggcagg atcgtcgagt gcctgcgggt gggcatcgct
     1501 cgtgcgctcg atcttccaca agatgcggag ctccctcgcc tcacccatca tgcccgtcaa
     1561 taccacttgc gaagtggact gcgggccgcg ctgatcggcg agcagcacgc tgtcctcgat
     1621 agccatgtgc tcggcgttag agctgatgcg ccacgtctcg ccattccgca ggatgagcgt
     1681 cacagcctcg ccgtcgccca tgagttgcgc cttcacactg gggtgcaggt gaaagcggat
     1741 cgcgtaggcg ccggcgatca ggccttcggg actcttgagc ccccgcggcg cggtaagctt
     1801 gtcctcggcg ctcagcagat tgccgtccgg cgagatcagg atctgccgcg agtggttcac
     1861 gccataccgg cccatatatc cgtcatggga tcccttgata cggagattgt caccttggtc
     1921 ggagaacgcg ccttgtgggt ttagcggtcc gaccattgtg gcgtccgcgg ccggctccgc
     1981 accattggcg ccagcacgga actctgcgag cgaatcttct tcgagactga gcgtggagtg
     2041 cgcgggcgtc gtgcgcgaaa acatccgcca cccttcttgg tcgggggatg gcgcgccgca
     2101 attaataatc acgggcgcat cgcctgagct catctcaaag gacaagaacc cggcatgcat
     2161 gttggttgtg agagacgcga ccggcggcgg tccgagatcg cagatcacga gcgtcgtgcc
     2221 cgcctcgacc cgcacatagc cggaattagc ggcggtccgc acgggcgcgc cttcgatgtc
     2281 gtcataggca agcacggtgg caagcgcgtc agtgggcgtg ggacccgcac cattaaagcg
     2341 cgacatggtg ccgtcgccca tgcggaagaa gcgcagcatc ggcatgatgc ggtcgatggc
     2401 atcggccaac tccttcggcg gggtgcggtc gcgtgccaca aaacattgcc gcaagggcag
     2461 aagatcgagc agcagctcga tcaacgcttc gggaatgcgt gagatgtgac cgccgtcggg
     2521 caggatttgg cggtccagct ccttgcacag cggcctggtg tagcggtcga cgaccgcctg
     2581 ctgatcggcg atgcaaagcc ccgcataaag gagccccatg aggctcagga ggcgcggcac
     2641 accgtcgggt gcatcgcgat agctggccga caaataacgc aattgcccgg tgagcgcctt
     2701 gaggaacgac tcgtaggcct gcggctcacc ggcatcgaga atgagaaccg agttggagag
     2761 ccaggaaatc acgcgccgcg ccacgatctc cggctgccag gcgagcccat ggacttgccg
     2821 gcgcaggcgg atgaagtcgt ggacgagcgc cttggcctgc tcgcgagata gctcgctgtc
     2881 ggcggcgcgc aaatggcgga gccagccgaa gccgtagagg tcgcgcgccc agttggcgga
     2941 cggaggcttg atctcgaagg gggagtccgc gccgagttcg gccagtttcc cggcgagccc
     3001 gaaatgcccg ttatagacct cgttggcgaa gcttggatcg gcggtgcgca gctcttgggg
     3061 cgccagcaga agctgctcgg cctggccccc cggatagctg atgctggcca tgctggagag
     3121 catcaagcgc cgcaaggggc tcggcccgcc aagatacacc tcttcaacgc gagaatcgcg
     3181 ctcgaaggcc tgatcacccg ccatatgggt catactccac tcgcccgcgc ctgtggtctc
     3241 gaggcgctgg cgtccccgcc gctctagcac ttagctgagg ctattttcat gcagcatgaa
     3301 ccccggggca agtgcgatgc cgtctttcgt caatcggcga gcctgagccg ggcggcgaag
     3361 aagccgtcca gaccgctcca gtccgggctt ccaaggtcaa gctggaaggg aaaggtgcgt
     3421 aagatcccgg acgcctcggc ccattcggta tcgcccaggg gctcatcttt gcggagagga
     3481 tcgatgcgga tggcgggatt gcgagccatc agcgccgcaa tttgagcctc gccctcctcc
     3541 ggctccagcg agcaggtcga atagacgagc gtgccgcctg gcttgagcaa cgagaccgcg
     3601 ttgtccagga gcctcgcctg gacgtctacc agctcggcga tgtccttgtc ggacttgagg
     3661 taggggatgt ccgggtgccg gcggatcgtg ccggtggagg cgcaaggggc gtccaggagg
     3721 atcgcgtcga accgctcctc cggctgccag gcgcctgcat cggcccgtac ggtctcgacg
     3781 gtgagcccga gccgcttcag attctcctcc agccgctcaa ggcgcgactt ggagtgatcc
     3841 acggcgacga cctcggcgcc cgcgagcgca agctgcgctg tctttccgcc cggcgccgcg
     3901 cagagatccg ccacgcgctt gccggtgagg tcgcccagga gctgcgccgg cagggaggcc
     3961 gccgcgtcct gcacccacca attgccgtcg atgaacccat cgagatcctc gatgcggccc
     4021 ttgggcagga tacgtacgct acccgtcggc aagacgcggc cgccaagccg cgccgcccaa
     4081 ccgtccgggt cggactttac cgtcagatcg agcggcggct cgaccagcgc agcctccgca
     4141 ctcgcccgcg tccgctcttc gccccaggcg cgcacccagc ggtcccacag ccagtccggc
     4201 gtgttgaccc gcgccgcatc gagctttgct gcgatctcgc cgcctttgtc ggctacgcgc
     4261 cgcagcacgg cgttgacgag cttgtcgtag cgtttggcgc gcggatccca tttggcaagc
     4321 cgcacggcaa ggtcgatggc cgcgtgcggc gggacgctca taaagagaag ctgcgccgcg
     4381 ccggacagaa ggatggcgta gagcgtgccg gcgcgcgacg gcagaccgcg ttccagaaac
     4441 gtgttcagta ccgcatcgat ttgtccctta tgccgcagac tcgtaccaac gatggcacgc
     4501 gccaaggcgc ggtcccgcag aggcaggtcg aacagatagc cgccgtctcg cgcgcgccca
     4561 agaacttcgt cgagcggctg cttcttctgc aacacggcat tgagaaggtc ggcggctgcg
     4621 cggcgtgccg gcagtccaac ctgatgatcg cgcgtgcgcg aaagactatt cgtctgggtg
     4681 ttcgatgtgg tcatgattga tctccaatgt gcttcggttt ccggcgtggg agggatcagc
     4741 tcccgcgcca atcaaggtgg ggcatctcag ccccaattgg cctagcccca aaagggctta
     4801 gccccaaggc ccacgcgtgg tgcgcgcggt tgcgccggac caaggacccg tgggctcagg
     4861 cgcaaggttc ggctcatgcg cgccgcccat ggtcttggac atcgcgacga gccgcgccac
     4921 ccggtcatcg gtggccgggt gcgtggagaa cagcttgtcc atgcgcaggc ccgacagcgg
     4981 gttgatgata aacatatgcg cggagctcgg gttacgctcg gcttctgcgt tcggaatgtg
     5041 gtgcgcggca ccggctatct tttgcagcgc cgaggcaagc cacagcggct ggccgctaat
     5101 ctccgcgccg ccgcggtccg cttcgaattc gcgcgtccgg ctgatcgcca tctgcacgag
     5161 acccgcggcg agtggcgcaa ggatgatgag cgcgatcgtg ccgaggatgc cgatgccgtt
     5221 attgtcgcgg cttccgccga agaacatcgc gaagttggcg agcatggaga tcgcaccggc
     5281 tatggtggcg gtgatggtca tgagcaacgt gtcgtgatgt ttgatatgcg ccagctcgtg
     5341 cgcgatcacg ccggcaagct cctcgcgtgt gacggactgc actaggccgg tggtcacggc
     5401 caccgcggcg ttcttagggt tccgccccgt ggcgaacgca ttcggctgcg ggttctccat
     5461 cacgtagacc ttcggcatgg ggagcccggc gttgcccgcg agctgctgca ccatgtcgta
     5521 aagcttcggg cttgtgcgcg cgtcgacctc gcgcgcgccg tacatgcgca gcaccatctt
     5581 gtcggagttc cagtaggaga acaggttcat gccggccgcg atcacgaacg cgatgatcat
     5641 gcctgtctgt ccgcccatga ggaatccgat ccccatgaac agcgccgtaa ggcccgcaag
     5701 caggatggcg gtgcggaaat aactcattga aatgcctatg acctccgata ggcggcaaaa
     5761 tgtgccgccc tctatatgtt gggagccgcg gccgattctg caagggcttg atcggcaagg
     5821 gaaaaatcga aagggtggcg ctgcgcccgc ctccgcttta agggcgcgct taccttaacg
     5881 cttcgtcaat cggaagcgcc cattctggcg ggcaagacat ttgaaggagt cgccgaccat
     5941 gccagccgcc ctgcaagctt cccagacagc caccaaggtc aagccgcgcc gcgcccggcg
     6001 cagcacgcga tccaaggata cggagctcac ggcggagcag aagcggctga tccgcgtgag
     6061 ctatctcaaa gtggagccgg cgctcgagct cgtcgcccag ctcttcttcc tcaagctgtt
     6121 ccgcctcgac ccgtccttac gcaagaagtt ctcagggccc gtcgagatgc aggcgcgcaa
     6181 gttcgccgcc ggcgccaagc ttgccatgat ttccctcggc cacgaggacg gcctgtcgcc
     6241 gaccctgaag ctgctgggga cgcgtcatcg gcaaatgggc atccgcacgc gtcactatcg
     6301 cactatgtcg cgcgctctgg tctggacgct ggagcggtcc ttagagaagg ccttcgaccg
     6361 cgacaccaag gatgcgtgga cgacgatgct cgcctatttc acgcgcacca tgtcggcgtg
     6421 agccgcgttc agcgcagcat ttagagatta gttcgaggct ccttcggggg cctcgtcgct
     6481 ttcccgcatg accttcacct gcagaccccc gttcttgccc acatagagca tcacgtcctt
     6541 gccgggctca agaacctgcg tcttgccgtc caccgaaagc tgatcgctgg agacgaccac
     6601 ctcgtggtca tcaacgacaa tctttatgcc atcggcgatg gtctcttgcg tcatgtcctt
     6661 gaagtgcccg gcgacgttga tcgtgcggtt gccgaccgcc aaggcgagcg cttgagtcgg
     6721 gtcgccactc gttccgagtc gcccgacgac ga
//
LOCUS       DBNHVU010000112         5794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_933277, whole genome shotgun sequence.
ACCESSION   DBNHVU010000112 DBNHVU010000000
VERSION     DBNHVU010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5794
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_933277"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="AC16Y7_04310"
     CDS             complement(<1..664)
                     /locus_tag="AC16Y7_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR5708638.1"
                     /translation="MNQRSYLDYNATAPLRPEVREAVADALGHAGNPSSVHAEGRAAR
                     AAIEEARGRVASLAGARPEDVIFTSGGTEANVTVLAEPAADESWQLYLSAIEHPSVLS
                     GGRFHPETTTMIPVTADGVADLAALAMTLEKHKPGGWRPLVSLMAANNETGAIQPVRE
                     AADIVHEAGGLLHSDAVQAAGRIPLDMTSLGTDMLTLSAHKIGGPKGVGTLVLKDGVR
                     VEP"
     gene            942..1613
                     /locus_tag="AC16Y7_04315"
     CDS             942..1613
                     /locus_tag="AC16Y7_04315"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5708639.1"
                     /translation="MPEVNISGPSGRIEARYHHQPSTSAPVALILHPHPQFGGTMNNQ
                     VVYHLYYAFQKRGFSVLRFNFRGVGRSEGLFDNGPGELADAATALDWLQGYNPDAPAC
                     WICGVSFGAWIAMQLLMRRPEIEGFISVAPPANLYDFTFLAPCPSSGLMVNGAKDRVV
                     PTESVRGLVDRLKTQKGIVIDHSTVPDANHFFEERMDGLMDVVQGYLDKRLGKPNKKK
                     KESAA"
     gene            1716..2969
                     /gene="tyrS"
                     /locus_tag="AC16Y7_04320"
     CDS             1716..2969
                     /gene="tyrS"
                     /locus_tag="AC16Y7_04320"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR5708640.1"
                     /translation="MSTVKSDFLHELQTRGYIHQCTDEAALDNLARSEVITGYIGFDC
                     TAPSLHVGNLVSIMLLRKLQQTGHKPIVLIGGATTKVGDPSGKDETRKLLTEEQIDAN
                     IGGIESVFSKFLAFGDGETDARLVNNTDWLEALNYVDFLRDYGRHFSVNRMLSQDSVK
                     LRLEREQNLSFIEFNYMVLQAYDFVELYRREGCRLQMGGSDQWGNIVTGIELGRRTES
                     AELFGLTTPLLTTASGAKMGKTAAGAIWLNPDMLSPYDYWQYWRNAEDADVPRFLKLF
                     TELPLPELDKLAALEGAEMNEAKKILATEATALVHGRDAADRAAETARTTFEQGQIAE
                     SLPSIDVPRGELEAGVGVLNASMLAGFVTSTGEARRHIKGGGIKVNDTPVTDARRVLT
                     LSDLTDDGVIKLSLGKKRHALIRPV"
     gene            complement(2982..>5794)
                     /locus_tag="AC16Y7_04325"
     CDS             complement(2982..>5794)
                     /locus_tag="AC16Y7_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HIR5708641.1"
                     /translation="RSILVGQANVASSRGDWQLEVRTEQRTRTNSLGITALIENLVPS
                     GIAGNFPSIGILKALDMAVDGKADAELSKSGDFLSGEAKLKLAPGQITPPWDRDTPVR
                     IDHGDFTVRYMPETDVVEIAPSTLRWGKSKATFSGEFRPVRDAQKKVVTWNFSIKGDD
                     AVFGVEEHGLEPITIDEWTAVGSLAPKDGHLTLSRFVIRSGDAQITASGKVVDAPDSP
                     AISLAGEVSPMPIDTLKLLWPKFVAGKAREWVLERVTGGQLLGGHFKVALPAGALAVL
                     EQGGDTPEGAVNVELNFKDTGIAYISELPPVVTGQGRLTITGTEFTVDIPRAKIVVAE
                     GQEIQLGEGRFYIPDLREDPQQGVVSFHAGAPTPTVMKLLDHEPLGYLQEVGMKPDFL
                     GGSAEGAFTLAMPLKHDLDFKEIKMSGQAQLNDAIASNIVGDMGIEGGALNVGLSEEG
                     VTARGDIKIKGVPAEIFWQRVFYAADEEQPPIQVSANLDENLRKQLGIKVNHLVKGTV
                     PLTLFVKGLGQGTQQLSMEADLGNAELLFGSMGWTKPAGKAAKVSFDVDQQDDGSTGL
                     DNFKIIGDDIAVHGKIALGPEQQLKSFYFSDFSVNELTHMEITAKVREDQVLVIHAEG
                     PSYDGRQFFRSLFSAGQLAEDGSAEPDDPFGIDFTAKIGRVIGFDDTYATDMDVMLKK
                     RSGRLTALRGKGKLNGKSAASAQLTMKNGARILKAESLDAGSVFRLIGFYRSIEGGQA
                     SLQVNMDAGGRGTKSGTLWATNFNVVGDRVVADVLTDPSSVAVLGQENKKHIARSKIK
                     FKRLRAPFVVGGGKFRLRDAYINGPQLGATMRGTVDFKSQTVALGGTYVPLYGLNSAL
                     GAIPILGRVFVGRQGEGVVGITFAIKGKLDDPTVLVNPMSVMAPGIFRQIFDFHGGGV
                     PAGRAAAKTKPEPTRTFQNPQY"
BASE COUNT         1084 a         1856 c         1766 g         1088 t
ORIGIN      
        1 aaggctccac acgcacgcca tctttcagga cgagtgtgcc gacacctttc ggtccgccga
       61 tcttatgggc cgatagggtc agcatgtcgg tgccgaggct tgtcatatcg aggggaatgc
      121 ggcctgccgc ctgcaccgca tcgctgtgca gaagaccgcc ggcctcatgc acgatgtcag
      181 ctgcctcgcg cacgggctga atcgcacccg tctcgttatt ggcggccatg agcgacacca
      241 gcggccgcca gccgcccggc ttgtgcttct ccagcgtcat ggcgagcgcg gcaagatcgg
      301 caacgccgtc tgcggtcaca gggatcatgg tcgtggtctc tgggtggaag cgtccgcccg
      361 acaacaccga cggatgctcg atggccgaga ggtagagctg ccaagactca tcggccgccg
      421 gctcggcaag gacggttacg ttggcctcgg tgccgccgct ggtgaagatc acgtcctcag
      481 ggcgagcgcc ggcaaggctc gcgacccgtc cccttgcctc ttcgatggcc gcacgagcgg
      541 cccgtccctc ggcgtgtacg gaggacggat tgccggcgtg cccaagcgca tcggccacgg
      601 cctcgcgcac ttcgggacga agcggcgcag tggcgttgta atcaagataa gagcgctggt
      661 tcatgttccg ccggtgctcg cggctgtatg ccgccggtta atgcttgcgc gtgcccccgt
      721 cgcacgtgct atgaagcgcg gcctgcaatg gaccactcca agcgcccagc tgatttggaa
      781 cggtcttgtt tggaagaatt ctaatcttct tgaatttgta taggtttccg gggccgcgaa
      841 gtcaaggatt ggcggcttgt ttggtacgtc cgctgcaacg ccccatatag aaatcttgcg
      901 acacatcgtc ggcgaaacaa cgcctgtgag gcccggagtt tatgcctgaa gtcaatatca
      961 gcggcccgag cggtcggatc gaagcccgct accaccatca gccgtctacg agcgcgccgg
     1021 tcgcgctgat tctgcacccg cacccgcagt tcggcgggac gatgaacaat caggttgtct
     1081 accacctgta ctacgccttc cagaagcgcg gcttctccgt gctgcggttc aattttcgcg
     1141 gcgtggggcg tagcgagggc ttgttcgaca acggacccgg cgaactcgcc gacgccgcca
     1201 cggccctgga ctggctccag ggctacaacc ccgatgcccc ggcttgctgg atctgtggcg
     1261 tgtcgttcgg cgcctggatc gccatgcagc ttctgatgcg ccgaccggag atcgagggct
     1321 tcatctccgt ggccccgccc gccaacctct acgactttac gttcttagcg ccctgcccgt
     1381 cctccgggct gatggtcaac ggcgccaagg accgcgtcgt gccgaccgaa tcggtgcgcg
     1441 ggctcgtgga ccggttgaag acgcagaagg gtatcgtcat cgaccattcg accgtgcccg
     1501 acgccaacca cttcttcgag gagcggatgg acggcctgat ggacgtggtg cagggctatc
     1561 tcgacaagcg cctcggcaag ccgaacaaga agaagaaaga gtcggcggcg taaggcggcg
     1621 ttctccccag ttcgccacgg ctccggattt ggtaatccgg cggcaaacct gatagaggct
     1681 ctggcgaaca ctatgagacg cccagcggcc tgcccatgag cactgtcaaa tcggactttc
     1741 tgcatgagct ccaaactcgg ggctacattc accagtgcac ggacgaggcc gcgctcgaca
     1801 atctcgcgcg gtcggaggtc atcaccggct atatcggctt cgactgcacg gcgccgagcc
     1861 tgcatgtggg caacctggtc agcatcatgc tgctacggaa gctgcagcag acgggacaca
     1921 agccgatcgt cttgatcggt ggcgccacca ccaaggtggg cgacccttcc ggtaaagacg
     1981 aaacgcgcaa gctcctcacc gaagagcaga tcgacgccaa tatcggcggg atcgaaagcg
     2041 tgttctccaa gttcctcgcc tttggcgacg gcgaaacgga cgcgaggctg gtcaacaaca
     2101 ccgattggct cgaagcgctc aactacgtcg acttcttgcg ggactacgga cgccatttca
     2161 gcgtcaaccg catgttgagc caggattcgg tgaagctgcg cctcgaacgc gagcagaact
     2221 taagcttcat cgagttcaac tacatggtgc tgcaagccta cgacttcgtg gagctctacc
     2281 gccgcgaagg ctgccggctg cagatgggcg gctccgatca atggggcaac atcgtcaccg
     2341 gcatcgagct tggacggcgc accgaaagcg ccgagttgtt cgggctcacg acgccgctac
     2401 tcacgaccgc ctcgggcgcc aagatgggca agacggcggc gggggcaatt tggctcaacc
     2461 cggacatgct ctccccttac gactattggc agtattggcg gaacgccgag gacgccgacg
     2521 taccgcgctt cctcaaactg ttcaccgaat tgccgctccc ggagctcgac aagctggcgg
     2581 cgcttgaagg cgcggagatg aacgaggcca agaagatcct ggccaccgaa gcgacggccc
     2641 tcgtgcacgg acgcgacgcc gccgaccgcg cggcggaaac ggcgcgcacc accttcgagc
     2701 aagggcagat cgccgagagc ctgccgagca tcgacgtgcc gcgcggggag ctcgaagcgg
     2761 gcgtaggcgt gttgaacgcc agcatgctcg ccggcttcgt gacgtcgacg ggcgaggcca
     2821 ggcgccacat caagggcggc gggatcaagg tcaacgatac gccggtgaca gatgcccgcc
     2881 gagtgctcac actgtcggac ctgacggacg acggcgtgat taagctctcg ctcggcaaga
     2941 agcgccacgc cctgatccgg cccgtctagg cggatcgtcc gctaatactg gggattctgg
     3001 aacgttctgg tcggttccgg cttcgtcttt gcggccgccc gtcccgccgg cacaccgcca
     3061 ccgtggaaat caaaaatttg ccggaagatt cccggtgcca tgaccgacat cggattgacc
     3121 agcacggtgg gatcatcgag cttacccttg atggcgaagg taatgcccac cacgccttcg
     3181 ccctggcgcc cgacaaacac gcggccaaga attggaatgg caccgagcgc ggagttgaga
     3241 ccgtagagcg gcacataggt gccaccaagg gcgacggtct gcgatttaaa gtccaccgtg
     3301 ccgcgcatgg tggcgccgag ctgcggtccg ttgatgtagg catcgcgtaa gcggaacttg
     3361 ccgccgccga cgacgaacgg cgcgcgcagc cgtttgaact taatctttga tctggcgatg
     3421 tgcttcttgt tttcctgacc gagcacggcg acggagctcg gatcggtgag cacgtcggcg
     3481 accacgcgat cgccaacgac attgaagttg gtcgcccata gggttccaga cttcgtgccg
     3541 cgcccgccag cgtccatatt gacctgaagc gaggcctgac cgccctcgat gctgcgatag
     3601 aatccaatca atcggaacac cgaaccggca tcaagcgact cggccttgag aatccgcgcg
     3661 ccgttcttca tcgtcagctg cgcactggcc gccgacttgc cgttcagctt gcccttaccg
     3721 cgcagcgccg tcagccggcc gctgcgcttc ttgagcatga cgtccatgtc cgtggcgtat
     3781 gtgtcgtcga agccgatcac gcgcccgatc ttcgccgtaa aatcgatgcc gaaaggatcg
     3841 tcgggctccg ccgagccgtc ttcagccagt tggcccgcag agaacagcga gcggaagaat
     3901 tgcctgccgt catagctcgg gccttcggcg tgaataacga gaacctggtc ctcgcgaacc
     3961 ttcgcggtga tctccatgtg ggtcagctcg ttcaccgaaa agtcggagaa atagaagctc
     4021 ttcagctgct gctctggccc gagcgcgatc ttgccgtgaa cggcgatgtc gtcgccaatg
     4081 atcttaaaat tgtcgaggcc ggttgagccg tcgtcttgtt ggtccacgtc gaagctgacc
     4141 ttcgccgcct tgccggccgg tttggtccaa cccatgctgc cgaacaagag ctccgcattg
     4201 cccaggtccg cctccatgct caattgctga gtcccctgcc ccagcccctt gacgaacagg
     4261 gtcagcggaa ccgtcccctt cacgaggtga ttgaccttga taccaagttg cttgcgcaaa
     4321 ttttcgtcga ggttcgcgct gacttgaatc ggcggctgct cctcgtctgc tgcgtagaac
     4381 acgcgttgcc agaaaatttc ggcgggcacg cccttgatct tgatgtcccc gcgcgccgtg
     4441 acgccttcct cggacaggcc cacgttcagc gcaccgccct cgatgcccat gtcgccaaca
     4501 atgttcgagg caatggcatc gttgagttgc gcctggccgc tcatcttgat ctctttgaaa
     4561 tcaagatcgt gcttcaacgg catggccagg gtgaatgccc cctcggcgct gcccccaaga
     4621 aaatcgggct tcatgcccac ttcctggagg tagcccaggg gttcatggtc gagaagcttc
     4681 atgaccgtgg gcgtcggggc accggcgtga aacgaaacga cgccctgctg cggatcctcg
     4741 cgcagatcgg gaatgtagaa gcgcccttcg ccaagttgga tctcttgccc ttcagccacg
     4801 acaatcttgg cgcgggggat atcgaccgtg aattccgttc cggtgatcgt gagtcttccc
     4861 tgacccgtga ccacgggcgg caactccgaa atataggcga tgcccgtgtc cttgaagttg
     4921 agctcgacat tgacggcgcc ttcgggcgta tcgccacctt gctcaaggac cgcgagcgcg
     4981 ccggccggaa gcgccacctt gaaatgcccg ccaaggagct ggccgccggt cacccgctcc
     5041 agaacccatt cccgcgcctt gcccgccaca aatttgggcc atagcagttt gagcgtgtcg
     5101 atgggcatgg gcgagacttc gccggcgagg ctaatcgccg gggaatcggg tgcgtcgacc
     5161 actttgcccg acgccgtgat ctgggcatct cccgaacgga tgacaaaacg cgacagcgtt
     5221 aagtgaccgt ccttcggcgc gagacttccc acggcggtcc actcgtcaat cgtgatgggc
     5281 tccagtccgt gctcctcgac gccgaacacg gcgtcgtcgc cctttatcga aaagttccag
     5341 gtgacgacct tcttttgggc atcacggacg gggcggaact cgccgctgaa cgttgctttg
     5401 ctcttgcccc agcgcagcgt cgaaggcgcg atctccacca cgtccgtctc gggcatgtaa
     5461 cgcacggtga aatcgccatg atcgatgcgc acaggcgtgt cacgatccca gggcggggtg
     5521 atctgtcccg gtgccagttt gagcttcgct tcgccggaca agaagtcgcc ggacttcgaa
     5581 agctccgcgt cggccttgcc gtccacggcc atgtcgagcg ccttgaggat gccgatggac
     5641 gggaagttac cggcaatccc ggaaggcacc agattctcga tcagcgcggt aatgccgagg
     5701 ctgttcgtcc tcgtacgctg ctcggtgcgc acttcgagtt gccaatcccc ccggctcgac
     5761 gcgacgttcg cttgccccac cagaatgctg cgct
//
LOCUS       DBNHVU010000113        19712 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1083651, whole genome shotgun sequence.
ACCESSION   DBNHVU010000113 DBNHVU010000000
VERSION     DBNHVU010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 19712)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19712)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19712
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1083651"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..411)
                     /gene="rpmG"
                     /locus_tag="AC16Y7_04330"
     CDS             complement(244..411)
                     /gene="rpmG"
                     /locus_tag="AC16Y7_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR5708642.1"
                     /translation="MAKPVTQKIKLVSSAGTGYYYVTKKNPRNQTEKLVMKKYDPVAR
                     KHVEFKEAKIK"
     gene            complement(475..924)
                     /locus_tag="AC16Y7_04335"
     CDS             complement(475..924)
                     /locus_tag="AC16Y7_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HIR5708643.1"
                     /translation="MPKSAGAHQLEGRSTGARPLSQSLLRGASLKCPACGTGALFRRY
                     LKVADNCAHCGESLHHHRADDAPAYFTIVIVGHVVVSLVLAVEVAYRPPMWLHAALWL
                     PLTVVLALVLLAPIKGTLVALQWALLMHGFDPDAKEEIGDRLAPPSA"
     gene            complement(945..3275)
                     /gene="rnr"
                     /locus_tag="AC16Y7_04340"
     CDS             complement(945..3275)
                     /gene="rnr"
                     /locus_tag="AC16Y7_04340"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HIR5708644.1"
                     /translation="MAKTKKATPKTKKKKAAAKQAGKLPSKQEIVDFLKSAPAKSGKR
                     EIARAFGVKGADRINLKNLLRTMADEGLITGSRRKLTRPGTLPPVTVLEITGRDNDGE
                     FVARPATWDDEHGPTPRILMVESKRDGAPAGVGDRVLARITALGADAEYPYQARTIKR
                     LPRTTSRSLLGIFRALKDGRGIIDPIDKKQLKEWTVPRGAAGEAKDGELVRFEIARSA
                     RYGVPTARIIERLGNPEAQQAISLIAVHAHGIPDVFPPAVIAEADAAKPVDLGRREDL
                     RDIPLITIDPSDARDHDDAVWAEPDPNPKNEGGWVVIVAIADVAHYVTPESALDTEGR
                     KRGNSVYFPDRVVPMLPERISNDLCSLKEGVARPCLAVRMIFDKSGHKLSHRFLRGLM
                     RSAASLRYEQAQGAIDGRPDETTGPLLDSVLRPLWGAYASVLRARTDRGPLDLDLPER
                     KILLDDKGRVRGVATPQRLDAHRLIEEFMIQANVSAAETLEAKRSPLIYRAHDAPSKE
                     KLSTLGEFLATIDIKLPKAGTLKPAQFNRILAETRGTPTAELVAEVVLRSQSQAEYNP
                     HNFGHFGLNLRRYAHFTSPIRRYADLTVHRALIRALDLGAGGSTDKELEALEEIAGAI
                     SDTERRAMAAERETNDRLIAAYLADRIGATFQARVSGLVRSGLFVRLLDTGADGFVPA
                     SGIGHEYFYHDEVRQALVGEDSGLAFQLGDPVEVRLAEAIPTAGALRFDMLSEGRRLA
                     TKPGRPRIKRPARGGRPARGKPRSGKKRGGKPRKRR"
     gene            complement(3285..5906)
                     /gene="topA"
                     /locus_tag="AC16Y7_04345"
     CDS             complement(3285..5906)
                     /gene="topA"
                     /locus_tag="AC16Y7_04345"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR5708645.1"
                     /translation="MNIVIVESPAKAKTVNKYLGPDYRVFASYGHVRDLPSKNGSVLP
                     DNDFEMHWDVDPSSAKRLADIVKALKGADKLILATDPDREGEAISWHVLQVLNGKKLL
                     DKVDVERVVFNAVTKDAVLDAMRHPRKIDEPLVNAYLARRALDYLVGFTLSPVLWRKL
                     PGARSAGRVQSVALRLVCDRELEIEKFKSQEYWSIVADLATGKNETFQARLVTADGKK
                     LEKFDIPDQANADRLKDALEGGRFSVGKIESKAQRRNPAAPFTTSTLQQEASRKLGFA
                     PRQTMQLAQRLYEGIDLGGEAEGLITYMRTDGVQIVPEAVSSIRDLVTGLYGNRYVPP
                     SPRQYQTKAKNAQEAHEAIRPTDFSKAPDKIGRYLDADSLKLYKLIWQRTLASQASSA
                     EIERTTIDIDVAGKDGNPYGVRATGSVIRFDGFLKIYEEGVDDAVGEDGALPALNEGD
                     ALNSNAIQAKQHFTEPPPRYSEATLIKKMEELGIGRPSTYASTLNVLREREYVRVDKK
                     RLFPEDKGRLVTAFLESFFKRYVEYDFTAGLEEKLDRISAGELEWKDVLRDFWRDFIG
                     AVDDIGDLRIAKVLDALNELLGPHVFPDTGEGDPRTCPACKEGQLSLKVGKFGAFVGC
                     SNYPDCRFTRQLAESGDKAALTDGKVLGTDSETELEVTVRTGRFGPYIQLGEATDGEK
                     PKRASIPKGTDPEAIDLAHALKLLSLPREVGIDPESGKPITAGYGRYGPYVQCDGKYA
                     SLSTPEEVFEVGANRAITLLAEKAANRRPRGPSVLKELGDHPDEGGKIQVLDGRYGPY
                     VKHGKINATVPKDRNPEQVTLEEAVELIRARAAKGPGKKRPAKKAAAKKKAAKKPAKK
                     SPRKSAKTKEPAGEAAS"
     gene            6200..6379
                     /locus_tag="AC16Y7_04350"
     CDS             6200..6379
                     /locus_tag="AC16Y7_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708646.1"
                     /translation="MSAGAASKDTGVTRRETAEYLASMLEGLRKSAHQAKLPFLAYLI
                     SVTLEEANNEKQKSE"
     gene            complement(6386..6820)
                     /locus_tag="AC16Y7_04355"
                     /pseudo
     CDS             complement(6386..6820)
                     /locus_tag="AC16Y7_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580695.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DprA-like winged helix domain-containing
                     protein"
     gene            complement(6796..7125)
                     /locus_tag="AC16Y7_04360"
     CDS             complement(6796..7125)
                     /locus_tag="AC16Y7_04360"
                     /inference="COORDINATES: protein motif:HMM:NF045295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708647.1"
                     /translation="MRCCLASSPSFFTFATPRTFSGSCRARRRALAKTSTERLNDAQR
                     LAWLRLIRSENIGPAVAAIEAVPYLSRRGGRQNIRLCTEAEAEAELKAARRAGMQLVA
                     RRSVPDR"
     gene            complement(7016..7645)
                     /gene="plsY"
                     /locus_tag="AC16Y7_04365"
     CDS             complement(7016..7645)
                     /gene="plsY"
                     /locus_tag="AC16Y7_04365"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIR5708648.1"
                     /translation="MPPSDDWAAVLPSTLGLCLFSFALGYLLGSIPFGLILTKLTGRG
                     DVRAIGSGNIGATNVLRTGSKGLAALTLLLDVLKGAAAVWIGSHFGPYAAMAGGLGAF
                     LGHLFPVWLGFRGGKGVATYIGVALGLFWPVGLGFCVMWLLVATATRYSSLAALISSA
                     ATVGTFAVLQMWPLAVLFGVLTVLLYIRHAANIQRLLQGEETRIGEDKH"
     gene            complement(7676..8974)
                     /locus_tag="AC16Y7_04370"
     CDS             complement(7676..8974)
                     /locus_tag="AC16Y7_04370"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR5708649.1"
                     /translation="MPSHGKLLTLVNARLIDPATGLDAFGGLLIEDGVIADLGAHIME
                     GSVAGAESLDCGGRTVAPGLIDMMVFGGEPGHEHRETLATASRAAAAGGVTTICCMPD
                     TDPVIDDVALVDFILRRARDTALVHVHPMAAMTRELKGEEMAEIGLLQDAGAVAFTNG
                     KSSVANARLMRNVLSYAKDYGALIVHHLEDASLAQEGVMNEGEVATRLGLRGVPAAAE
                     TMMLERDIRLVELTGGRYHAGQISCRASLDVIRAAKSRGLPVTCSVSVNHLTLNENDI
                     GPYRTFFKMKPPLRAEDDRQAMVEGVASGDIDVIVSAHDPRGAEGKRRPFAEAADGAA
                     GLETMLGAALRLYHNGEVTLQRLLEAMSTTPARLLGLDSGRMAKGAPADLTLIDLEMP
                     WRVELEQLCSKSKNTPFEGARFEGRALVTFVAGRPVYHYG"
     gene            complement(8980..9936)
                     /locus_tag="AC16Y7_04375"
     CDS             complement(8980..9936)
                     /locus_tag="AC16Y7_04375"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041536035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIR5708650.1"
                     /translation="MSIAEEAPTAPFPHRHLLGIEGLSRSEIVTLLDLSEEAAKLGRQ
                     VNKKSDDLRGRTLINLFFEASTRTQSSFELAGKRLGADVMNMSIPTSSIQKGETLIDT
                     AVTLNAMRPDLLVVRHRAAGAVELLAQKVDCCVINAGDGSHEHPTQALLDALTIRRHK
                     NRIEGLIVAICGDIQHSRVARSNILLLNTLGARVRVIAPSTLLGAGIERLGVEVYNSM
                     TDGLADADIVMMLRLQRERMVSSLIPSQREYFHFYGLDYDKLACAKPDALIMHPGPMN
                     RGVEIDSNVADDSRSVIREQVEMGVAVRMAVLQALARHLPNR"
     gene            10294..10449
                     /locus_tag="AC16Y7_04380"
     CDS             10294..10449
                     /locus_tag="AC16Y7_04380"
                     /inference="COORDINATES: protein motif:HMM:NF039621.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5993 family protein"
                     /protein_id="HIR5708651.1"
                     /translation="MMSLPFVLFACAVGAAWMRQRGVSMGFWALGLIAMLVLFRFHVT
                     EVLMIQL"
     gene            10469..11020
                     /locus_tag="AC16Y7_04385"
     CDS             10469..11020
                     /locus_tag="AC16Y7_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HIR5708652.1"
                     /translation="MSADLSKLLNAIGLLAISTVLALALVDQLWFGDLPCPLCILQRA
                     GFFAAGFGIALNLIFGPKPSHYGVAIVGAIAGGSISLRQIVLHIIPGTGHYGNDVFGL
                     HLYTWAFIAFAAIILGCAIMLLDDRQFSRAEPMSVRLKVLPLSALLLFLTLAIANIAS
                     TVALCGAGLCPDDPKTYMLLQGG"
     gene            complement(11027..11509)
                     /gene="ruvX"
                     /locus_tag="AC16Y7_04390"
     CDS             complement(11027..11509)
                     /gene="ruvX"
                     /locus_tag="AC16Y7_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR5708653.1"
                     /translation="MTDTSPLPIEDLPSALQPGARLLGLDVGTKTVGLALTDVTRTVA
                     TPYKTLRRTKFTADAKEIAEIAEKEGVGGLVIGLPLNLDGSEGPRAQSTRAFARNLAA
                     HVPIPMTFWDERLSTAAVERHLIEADASRKRRAEVIDRMAAAYILQGALDRLRGLANS
                     "
     gene            11533..12234
                     /locus_tag="AC16Y7_04395"
     CDS             11533..12234
                     /locus_tag="AC16Y7_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR5708654.1"
                     /translation="MKLTWFGHSCFRVETGDSRILIDPFLSGNPTFEKANIPWTDVTE
                     GVTHVALTHGHEDHVGDTVRVCKETGTTLLAVHELATYLAGQGVEKTDPGNTGGMIPQ
                     GDFDLTFVQALHSSSNMVDGRPVYLGNPCGLVIAGKGDKTLYHLGDTDIFADMALINE
                     LHQPQIGIVPIGDRYTMGAKGAALACKRFFDFETVVPCHYGTFPVIDQTADKFVAEMG
                     GNRVLVPEVGVPFEV"
     gene            12344..12631
                     /gene="gatC"
                     /locus_tag="AC16Y7_04400"
     CDS             12344..12631
                     /gene="gatC"
                     /locus_tag="AC16Y7_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR5708655.1"
                     /translation="MSVDSATVRRIARLARIAITDEEAARLETELSGILDWVAQLDEL
                     DTSNVEPMTRVEAMSMKKRKDEVTDGFCADDIVKNAPEVDDHFFVVPKIVE"
     gene            12638..14125
                     /gene="gatA"
                     /locus_tag="AC16Y7_04405"
     CDS             12638..14125
                     /gene="gatA"
                     /locus_tag="AC16Y7_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR5708656.1"
                     /translation="MTDLTDLTIADARDALRDKSFSAEELAQAHLDAVDAANAGINAY
                     VLPTPQRALEMAKASDARLAKGEGGALEGIPLGIKDLFCTRDVRTTACSHILDGFTPP
                     YESTVTANLWGQGAVMLGKLNNDEFAMGSSNETSYYGDVVNPWRREGSDAKLVPGGSS
                     GGSSAAVAARLCLGATATDTGGSIRQPAAFTGTVGLKPTYGRCSRWGIVAFASSLDQA
                     GPITRSVRDAAIMLNVMASHDPKDTTSADVPVPDFEAAVGKGVKGLTIGIPKEYRVEG
                     MAPEVEALWQQGIAWLKDAGANIKEVSLPHTKYALPSYYIVAPAEASSNLARYDGVRY
                     GLRETGSDVTSMYENTREAGFGAEVKRRILIGTYVLSAGYYDAYYLKAQKVRTLIKRD
                     FDQAFEGVDAILTPTTPSPAFGVGEKSGDPLEMYLNDIFTVTVNMAGLPGVSIPAGLS
                     SEGTPLGLQLIGRAFDEETLFRAGQVIEDAAGPMAKPQDWWSKGA"
     gene            14125..14436
                     /locus_tag="AC16Y7_04410"
     CDS             14125..14436
                     /locus_tag="AC16Y7_04410"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HIR5708657.1"
                     /translation="MATRDAQKCDNMDEVRLEIDRIDQAMVDLICERFAFVDRAWQLK
                     NSPADARVPWRIQQVIDRVRARAEENNLPPELVEALWRQMIGWFIQYEEEKLRKPGPK
                     E"
     gene            14446..15918
                     /gene="gatB"
                     /locus_tag="AC16Y7_04415"
     CDS             14446..15918
                     /gene="gatB"
                     /locus_tag="AC16Y7_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR5708658.1"
                     /translation="MNAKSEKSNLIEGDTGDWEVVIGMEVHAQVSSNAKLFSGASTEF
                     GGDPNSHVSLVDAAMPGMLPVINEACVSQAVRTGLGLKAAINLYSVFDRKNYFYPDLP
                     QGYQISQFKNPIVGEGEVIVDLLSGERVIVGIERLHLEQDAGKSLHDQHPQHSYVDLN
                     RSGVALMEIVSKPDLRSSEQAKAYVSKLRTILRYLGTCDGNMEQGSLRADVNVSVRCP
                     GDELGTRCEIKNVNSIRFIGQAIEYEARRQIEIIEDDGTIDQETRLFDAKTGETRSMR
                     SKEEAHDYRYFPDPDLLPLELSQDFVDGLAADLPELPDQKRARFIADYGLTPYDADVL
                     VAERESVDYFEAVAKGRDAKQAANWVINELFGRLNKEGLTIFDSPVSAAQLGGILDLI
                     KEDTISGKIAKDVFDIVWTDGGDPSEIVEARGLKQVTDTGAIEKAVDEVIAANPDKVE
                     QAKEKPALIGWFVGQVMKATGGKASPQAVNALLKSKLGVD"
     gene            15933..16907
                     /locus_tag="AC16Y7_04420"
     CDS             15933..16907
                     /locus_tag="AC16Y7_04420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014328319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase family protein"
                     /protein_id="HIR5708659.1"
                     /translation="MTHAIRVHEYGGPETLTWEEVEVGEPGPGEIRVKQTAIGLNYID
                     VYVRTGLYPQDALPFVPGMEGAGVVTALGEGVRDLKVGRRVAYAGPIGGYAEERLIAA
                     DRVVRVPQGVSDETAAAIMLKGMTAQYLLRRTYKVGPDTTLLFHAAAGGVGMIACQWA
                     AALGAKVIGTVGSAGKALIARSHGCTHVINYREEDVVAKVQAYTKGEGVDVVYDSVGK
                     DTFPASLDCLKRRGMWVSFGQASGPVPEFRINLLAQKGSLFATRPALGDYIATRKELV
                     SCANDLFGAIAKNKVKIAIDQTYPLREATRAHGDLEGRLTTGSTLLIP"
     gene            17213..17488
                     /locus_tag="AC16Y7_04425"
     CDS             17213..17488
                     /locus_tag="AC16Y7_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708660.1"
                     /translation="MARLELSTAITGHMEFAAQAGAPDALFELGMLYATGRDIDMDLV
                     SAHKWFNLAAARGNVSAKTYRVELSQEMTTDQISEAQKQAREWLQTH"
     gene            complement(17539..18198)
                     /locus_tag="AC16Y7_04430"
     CDS             complement(17539..18198)
                     /locus_tag="AC16Y7_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708661.1"
                     /translation="MTHQKGVSAMRGPFHRGPFVLAGLTAAATMWLALPAPAAGADYC
                     VTCKSPSETYRCRVTGSGVKTSDALKLYCVIRTAKEGNHASCSAEKATAACVGLVKAY
                     TYDGPAIPENVTSDPRVRELQQRIERDQRTFKEPEGDEPTSLFELGGRAVDASKKGLR
                     NAGSAIGIGSSEPAARAPAPPPKTTRSAGAESESLARRSYRCMRSFFRNCGSTDKGAL
                     E"
     gene            18433..18525
                     /locus_tag="AC16Y7_04435"
     tRNA            18433..18525
                     /locus_tag="AC16Y7_04435"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18467..18469,aa:Ser,seq:gct)
     gene            18567..19073
                     /locus_tag="AC16Y7_04440"
     CDS             18567..19073
                     /locus_tag="AC16Y7_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708662.1"
                     /translation="MTPDPLKSDPEQDLDRAFIERERNRFAGRALSFLVLLNGAAALI
                     LLAIMAQAPESTVDGKVATALLFFSSGAMAALLAAFLAYINRTVRMRQSARREELRAA
                     LRGLAIAAVIGSGAAFLVGMNMVAMSAVEKSSSHPKGQREDKPAKSAPIEKVDDRGGA
                     QDQATRFG"
     gene            complement(19086..19283)
                     /locus_tag="AC16Y7_04445"
     CDS             complement(19086..19283)
                     /locus_tag="AC16Y7_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708663.1"
                     /translation="MRFLVGFILGVALTIIAVYVLDRDGGGTEEKRIVNWDVVSEKVD
                     ALTAEGQKAWADFTREMTGPE"
     gene            19466..>19712
                     /locus_tag="AC16Y7_04450"
     CDS             19466..>19712
                     /locus_tag="AC16Y7_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708664.1"
                     /translation="MAALLDRDEWRAFSDFLALGGAALCVILGLVLALAGEDPVIAFH
                     GCVLLGAAALAFFFILTQIMEKKETHDATNYADGVVRA"
BASE COUNT         3541 a         5947 c         6451 g         3773 t
ORIGIN      
        1 cgttatcgct ttgaacgcac aacaccctac ggagtgctca ggtccgccgc atctcctggg
       61 tccgtggggt ttggccggtt ggagcgcggt tgagtggcct cctcgttagc gccgcgtttc
      121 gaccggccac cttattccgg tgacggtctt tcccttcaga tcttgaactg ccggccggcc
      181 cgtgagggcg gggcatgccc ctgatcgaag gtgcggtgct gtcggtaagg cgcgcgcgat
      241 gcgctacttg atcttggctt ctttgaactc gacgtgcttg cgcgcgaccg gatcgtactt
      301 cttcatgacg agcttttcgg tctgattgcg cgggttcttc ttggtcacgt agtagtagcc
      361 cgtgccggcg ctactgacga gtttgatctt ctgggtgacg ggcttagcca tgtcgtcctc
      421 ggataaacgg taaagggaaa tttggcgcgc aacctatcgg gtcgcgagcg gaagtcaagc
      481 cgacggcggg gcgagccgat cgccgatctc ctccttcgca tcggggtcaa atccatgcat
      541 aaggagggcc cattgcaggg ccacgagggt ccccttgatc ggcgccagca gcacgagggc
      601 caggaccacc gtgagcggga gccacagcgc ggcgtgaagc cacatgggcg ggcggtaggc
      661 cacctcgacg gccagaacga ggctcaccac gacatggccg acgatgacaa tcgtgaagta
      721 ggccggcgcg tcgtcggcgc ggtgatggtg caaggactcg ccgcaatggg cacaattgtc
      781 cgcgaccttg aggtagcggc gaaacagggc tccggtaccg caggcagggc atttcagcga
      841 ggcgccgcgg aggagggact gcgatagcgg acgggccccc gtcgatcggc cttcaagctg
      901 gtgagcgcca gcgctctttg gcattggttt tcctcccaag cggatcagcg ccgcttgcgc
      961 ggcttgccgc cgcgcttctt gccactgcgt ggcttgcccc gagcgggccg gccgccacgg
     1021 gccggtcgct tgatccgtgg acgtcccggt ttggtagcaa gccgccgccc ttcgctcaac
     1081 atgtcaaaac gtagcgctcc tgcggtcggg atggcctcgg ccaggcgcac ttcgaccgga
     1141 tcgccaagct ggaaggcgag cccggagtcc tcgcccacga gggcttggcg gacctcgtca
     1201 tggtagaagt actcgtgccc aatgcctgag gcggggacaa acccgtccgc gccggtgtcc
     1261 agaagccgca cgaaaagtcc ggaccggacc aggcccgaga cccgggcctg gaaggtggcg
     1321 ccgatgcggt cggcaagata ggccgcaatg agccggtcgt tggtttcccg ttccgccgcc
     1381 atggcacggc gctcggtgtc ggagatcgcg cccgcgatct cctcaagcgc ctccaactcc
     1441 ttgtccgtcg agccgccggc gccgaggtcg agggcgcgga ttagcgcccg gtggaccgtg
     1501 aggtcggcat aacgccggat cggcgaggtg aagtgcgcgt agcggcgcag gttgagaccg
     1561 aaatgtccga aattgtgcgg attgtactcc gcctgcgatt ggctcctgag cacgacctcg
     1621 gcaaccagtt cggcggtggg cgtgccccgg gtctcggcca gaatgcggtt gaactgggcc
     1681 ggcttcagtg ttccggcctt cggcagcttg atgtcgatgg tcgcgagaaa ctcgcccaat
     1741 gtcgagagct tctctttcga gggcgcgtcg tgggcgcgat agatgagggg gctgcgctta
     1801 gcctccagcg tctcggctgc ggagacgttt gcctggatca tgaactcttc gatcaagcga
     1861 tgcgcgtcga ggcgctgcgg cgtggcgacg ccacggaccc ggcccttgtc gtcgagcaga
     1921 attttgcgtt ccggcaggtc gagatcgagc ggcccgcggt cggtgcgggc gcgaagcacg
     1981 ctggcgtagg caccccagag cgggcgtaag acggaatcga gcaggggccc cgtggtctcg
     2041 tccgggcgcc cgtcgatggc tccttgcgcc tgctcatagc gcaggctcgc ggccgatcgc
     2101 attagtccgc gcaagaagcg atggctcagc ttgtgtccac tcttgtcgaa gatcatgcgg
     2161 accgcgagac aaggccgcgc cacgccttcc ttcagtgagc agaggtcgtt cgaaatgcgc
     2221 tcgggcagca tcggcacgac gcggtccggg aagtagaccg agttgccgcg cttgcgccct
     2281 tcggtgtcga gcgcgctctc cggggttacg taatgggcga cgtcggcaat ggcgacgatg
     2341 acgacccaac cgccttcgtt cttcgggttt gggtcgggct ccgcccaaac cgcgtcgtcg
     2401 tgatcgcgcg cgtcggacgg atcgatggtg atgagcggaa tgtcccgcag gtcttcgcgc
     2461 cggccaagat cgaccggctt ggccgcgtcg gcctcggcga tcaccgcagg ggggaagaca
     2521 tcgggaatgc catgcgcgtg aaccgcgatc aggctgatgg cttgttgtgc ttccgggttt
     2581 ccgagtcgct cgatgatgcg agccgtcggc acgccgtagc gagccgagcg cgcgatttcg
     2641 aaacgcacaa gttcgccgtc cttcgcttcg ccggccgcac cgcgcggcac agtccactct
     2701 ttcagctgct tcttgtcgat cgggtcgatg atgccccgcc cgtctttcag cgcgcgaaag
     2761 atccccagca ggctcctcga cgtcgtgcgc ggcaggcgtt taatggtccg cgcctggtag
     2821 ggatactcgg cgtccgcgcc aagcgcggtg atccgcgcca gtacgcggtc gcccacaccc
     2881 gccggggcac cgtcgcgttt gctctccacc atgaggatac gtggggtggg accgtgttcg
     2941 tcgtcccacg tcgcgggccg tgcgacaaac tctccatcat tgtcgcggcc ggtaatctca
     3001 aggacggtga caggggggag ggtgcccggg cgtgtcagct tgcgccggga cccggtgatg
     3061 agcccttcgt cggccatggt gcgcagaagg ttcttcaagt tgatgcggtc ggcgcctttc
     3121 accccgaagg cgcgcgcgat ctcgcgcttg cctgacttcg caggcgcgct tttcagaaag
     3181 tcgacgatct cttgcttgga ggggagcttg cccgcctgtt tggcagcggc cttcttcttc
     3241 ttcgtctttg gggtagcctt cttggttttc gccacgtgtg ccgcttagct tgccgcctct
     3301 ccggcgggct ccttggtctt tgcgctcttg cgcggggact tcttggccgg cttctttgcc
     3361 gctttcttct tcgctgcggc tttcttggct ggccgtttct tcccaggccc cttagcggca
     3421 cgtgccctaa tcaactcaac cgcttcctca agcgtgacct gttcggggtt ccgatccttc
     3481 ggaaccgttg cgttgatctt gccgtgcttc acatagggac cgtagcggcc gtccaatacc
     3541 tggatcttcc cgccttcgtc cggatggtcg ccgagttcct tgagaacgct cggtccgcgc
     3601 gggcggcggt ttgccgcttt ctccgcaagt agggtgattg cgcggttggc gccaacctcg
     3661 aacacttctt caggcgtgct caagcttgca tatttgccgt cgcattggac atagggtcca
     3721 tagcggccat agccggcggt aatgggtttg ccgctttcag ggtcgatgcc gacctcgcgg
     3781 ggaagcgata acagtttcag cgcgtgcgcc aagtcgatgg cctcgggatc ggttcccttg
     3841 gggatcgagg cgcgcttcgg cttctcgccg tcggttgctt cgccgagttg gatatagggt
     3901 ccgaagcgtc ccgtacgcac ggtgacctcg agttccgtct ccgaatcggt acccagcact
     3961 ttgccgtctg taagcgcggc cttgtcgccc gactcagcga gctgacgcgt gaagcggcag
     4021 tcgggatagt tcgagcagcc gacaaaggcg ccgaacttgc cgaccttgag gctgagctgg
     4081 ccttccttac aggctggaca ggtgcgcggg tcgccttcgc ccgtatcggg gaacacatga
     4141 ggtccaagca gctcgttgag ggcgtcgagt accttggcga ttctgagatc gccgatatcg
     4201 tccaccgcac caatgaagtc gcgccagaaa tcgcgcagta cgtctttcca ttccagctca
     4261 ccggcggaga tgcgatcgag cttctcctca agaccggccg tgaagtcata ctccacgtag
     4321 cgcttgaaga agctctccag gaaggccgtc acgagccgtc ccttgtcctc ggggaagagg
     4381 cgcttcttat ccacgcggac atattcccgc tcgcgcaata cgttgagcgt agaggcatag
     4441 gtagagggcc ggccgatccc gagctcctcc atcttcttaa tgagggttgc ctcgctgtaa
     4501 cgcggcggcg gctcggtgaa gtgctgcttt gcctgaatgg cgttggagtt gagcgcatcg
     4561 ccttcattca gcgcgggcag ggcgccgtcc tcgccgacag cgtcatcgac gccttcttcg
     4621 tagatcttga ggaagccgtc gaagcggatg accgaacctg tggcgcggac gccataaggg
     4681 ttgccatcct tacccgccac atcgatgtcg atggtggtgc gctcgatttc ggcgctggag
     4741 gcttggctgg caagcgtgcg ctgccagatg agcttgtaga gcttcagcga gtccgcatcc
     4801 agatagcggc caatcttatc gggcgccttg ctgaaatcgg ttgggcggat tgcttcgtgg
     4861 gcttcctgtg cgttcttcgc ctttgtctgg tattggcgcg gcgaaggcgg cacgtagcgg
     4921 ttgccgtaga ggccggtcac cagatcgcgg atcgacgaaa cggcttcggg cacgatctgc
     4981 acaccgtccg tccgcatata tgtaatgagt ccttccgcct cgccgcctag gtcgatgcct
     5041 tcataaagcc gttgcgccag ttgcatggtc tggcgcggcg cgaaaccgag tttgcgcgag
     5101 gcctcttgct gcagcgtcga ggtggtgaag ggtgcggctg ggttgcggcg ctgcgccttg
     5161 ctctcgatct tgccgaccga gaaacgtccg ccctcaagcg cgtccttcag acggtcggca
     5221 tttgcctgat ccggaatgtc gaacttctcg agtttcttcc cgtcggctgt gacaagccgc
     5281 gcttggaaag tttcgttctt gccggtcgca aggtcggcga caatcgacca gtactcttgc
     5341 gacttgaact tctcgatctc cagctcgcgg tcgcagacga ggcgcaaggc gacggactgc
     5401 acgcggccgg cagagcgggc gcctggcagc ttgcgccaaa ggaccggcga aagggtgaag
     5461 cccacgagat agtcgagggc acgccgtgcg agataggcat tgaccagcgg ctcgtcgatc
     5521 ttgcgcgggt ggcgcatcgc atcaaggacg gcatccttgg tcacggcatt gaagaccacg
     5581 cgctccacat cgaccttatc gagcagcttc ttcccgttga ggacctgcag cacgtgccat
     5641 gagatggcct cgccctcacg gtcggggtcc gtggccagga tcagcttgtc ggcccccttc
     5701 agggctttca cgatgtcggc caggcgcttt gccgagcttg ggtcgacgtc ccaatgcatc
     5761 tcaaagtcgt tgtccggcag caccgaaccg ttcttcgagg ggagatcccg gacatgccca
     5821 taggaggcga acacccgata gtcggggccg agatacttgt tgacggtctt ggccttagct
     5881 ggcgattcga cgatgacgat gttcattgag acgttcctag ccgcgcaaaa atggcgttcg
     5941 gctgtagtta tgggtcctga atcgaccctg gcaggggcag aaaatggtgt ctccacccgc
     6001 gcctgtcaaa ttcagcgcgc tgacgattta caacttatag tagtctatat tattttaaca
     6061 actaaagagt gttaaaccag agtgtgctct tggtgcggca aacgctgcca gatccaacaa
     6121 tttattgggg gtggtagtgg gaaccagcgg gcgcagtgca aggcggaatg gaatggcagg
     6181 ctcagggccc cggcctccga tgagtgcagg cgcagcaagc aaggatacgg gggtcacgcg
     6241 ccgcgagacc gccgagtacc tcgcgtcgat gcttgaagga ctgcggaagt cggcgcatca
     6301 ggcaaagctc ccatttctgg cctatctgat cagcgtgacg ctggaagaag caaacaacga
     6361 aaaacagaag tccgagtgag cggcctcacg gctcgaccag cgagacgagt tgctggccgt
     6421 ggcgctggag acgacccgcc aggtcgagct caagcaggac aatatggacc ttccgcgcgt
     6481 ccagccccgt ggcgcggatc aattcgtcga tatctacggg acttgggcct agtgcctcca
     6541 ggatgcgctc ccgctgggtc tgtttgatgt cgggaagtgg cgcgggtgtg accggctaat
     6601 cggaggaggc gagttcggca ggcggcggga cgcggccgag gatcggtgca agcaacgcga
     6661 tgatatcgga cggctcggtg accagcgttg ccccttgctt caagagatta ttggtcccgg
     6721 cggcgcgtgg atcgagcgga cttcccggaa cggcgaacac ctcgcggcct tgctcgcccg
     6781 ccaggcgcgc cgtgatcagc gatccggaac gctgcgtcgc gccacgagtt gcatgccggc
     6841 ccggcgggcc gctttcagct cggcctctgc ttccgcttcc gtgcagaggc ggatgttttg
     6901 acggccgcca cggcgggaca aatatggcac ggcttcgatg gcggcaacgg cggggccgat
     6961 attttcgctg cggatcagcc gcagccaggc caaacgctgc gcatcgttga gacgctcagt
     7021 gcttgtcttc gccaatgcgc gtctcctcgc cctgcaggag ccgctgaatg ttcgcggcgt
     7081 ggcgaatgta aagaaggacg gtgaggacgc caaacagcac cgcaagcggc cacatctgga
     7141 ggacggcaaa ggttcccacg gtggctgcgc tggagatgag agctgcaaga gacgagtagc
     7201 gcgtcgcggt ggccacgagc agccacatca cgcagaagcc gaggccgacg ggccaaaaca
     7261 gcccgagcgc cacgccgata tatgtggcga cgcctttgcc gccgcgaaag ccaagccaca
     7321 cgggaaagag atgaccgaga aacgcgccga gaccgccagc catcgcggcg tagggaccga
     7381 aatgcgaccc gatccacacg gccgctgcac ccttcaacac gtcaagtagc agtgtcaggg
     7441 cggcgaggcc cttactgccc gtccgcaaga cattggtggc gccgatattg cctgagccga
     7501 tggcgcgcac atcgccacgg ccggtcagct tcgtgaggat caacccgaag ggaattgaac
     7561 caagcagata cccgagcgca aagctgaaca ggcacaggcc aagcgtggat ggaaggacag
     7621 cggcccaatc atcggacggc ggcatcgctt accctcattg tgacgccaga atcggtcaac
     7681 cgtagtggta gacgggccgt ccggcaacga aggtcacgag cgcccggcct tcgaatcgtg
     7741 ccccctcaaa gggcgtattc ttcgatttgg agcagagctg ttcgagctcc actcgccacg
     7801 gcatttcgag atcgatgagc gtgagatccg ccggcgcgcc cttcgccatc cgtccggagt
     7861 cgagaccaag gagcctggcg ggcgtggtgg acattgcttc cagcagacgt tgcagcgtga
     7921 cttcaccatt gtgatagagc cgcaaggcgg cgccgagcat ggtctcgagg ccagccgcac
     7981 catccgccgc ctcggcgaag ggccggcgtt tgccttccgc accgcgggga tcgtgggcgg
     8041 agacgatcac gtcgatatcg ccgctcgcga cgccttccac catggcttgc cggtcatctt
     8101 cggcgcgaag cggcggcttc atcttgaaga aggtccggta gggcccgatg tcgttctcgt
     8161 tcaacgtgag atggttgacg gagacgctac aggtcacggg gaggccgcgg gatttcgcgg
     8221 cacgaatgac gtcgagcgag gcgcggcagg aaatctggcc ggcgtggtag cgtccgcccg
     8281 tcagctcgac gagacggata tcgcgttcca gcatcatggt ctcggcggct gccggtacgc
     8341 cgcgcaggcc cagccgggtt gcgacctcac cttcgttcat cacgccctcc tgggcgagcg
     8401 aggcatcctc aaggtgatgc acgatgagcg cgccatagtc cttggcgtat gacagcacgt
     8461 tccgcatcag tctggcgttg gcgacgctcg acttgccatt ggtgaacgcg acagcgcccg
     8521 catcctggag caagccgatc tcggccatct cctcgccctt cagttcgcgc gtcatggcgg
     8581 ccatggggtg gacatggacg agggcggtgt cgcgggcgcg gcgcagaatg aagtccacca
     8641 gggccacgtc gtcgatgacg ggatcggtgt ccggcatgca gcagatggtg gtgacgccgc
     8701 ccgccgccgc cgcgcggcta gctgtcgcca gcgtctcgcg gtgctcgtgg ccgggctcgc
     8761 cgccgaacac catcatgtcg atgaggcccg gcgctacggt ccggccgccg caatcgagcg
     8821 actcggcgcc tgcaacggag ccttccatga tgtgcgcgcc gagatcggcg atcaccccgt
     8881 cttcgatcag caagccgcca aaggcgtcga gccccgttgc cgggtcgatc aagcgtgcgt
     8941 tgactagggt gaggagtttg ccgtggctgg gcattgcggt caccggttgg gcaggtgacg
     9001 ggcgagcgct tgcagtaccg ccatgcgtac ggcgacgccc atctccacct gttcgcggat
     9061 cacgctgcgc gagtcgtccg ccacgttgga atcgatctcc accccgcgat tcatcggccc
     9121 gggatgcatg atgagtgcgt cgggcttggc gcaggcgagc ttgtcgtagt caagaccgta
     9181 gaagtggaag tactcccgct ggctcgggat cagacttgag accatgcgtt cgcgctgaag
     9241 ccgaagcatc atgacgatgt cagcatcggc gagcccgtcg gtcatggagt tgtagacctc
     9301 cacgccgagc cgttcgatgc cggcgccgag aagcgtcgaa ggcgcgatga cgcgcacgcg
     9361 tgcgccgagc gtgttcagca ggaggatgtt cgagcgggcc acccgcgagt gctgaatgtc
     9421 accgcagatg gcgacgatga gcccctcgat ccggttcttg tggcggcgaa tggtgagggc
     9481 gtcgagcagg gcctgcgtcg ggtgctcgtg gctgccgtcg ccggcattga tcacgcagca
     9541 gtccactttt tgggcgagca actcgacggc gccggcggca cgatggcgga cgacgagcag
     9601 gtcggggcgc atggcgttca gcgtcaccgc cgtgtcgatc agcgtctcgc ccttttggat
     9661 cgacgaggtc gggatcgaca tattcattac gtcggcgccc agccgcttgc ccgccagctc
     9721 gaacgagctt tgggtccggg tggaggcctc gaaaaagaga ttgataagcg tccggccgcg
     9781 gaggtcgtcg ctcttcttat tgacctgacg gccaagcttc gccgcctcct cggagaggtc
     9841 cagcagcgtt acgatctcgc tgcgtgaaag accttctatg ccaagaagat ggcggtgtgg
     9901 aaacggggct gtgggagctt cttccgcgat gctcattaaa ggtcactgta taaggccgct
     9961 cggtccaggc atcaagcgtg agcgcatatt tcgggaagct tggtgaaagc aggccatgtg
    10021 cgatgagagc acggtgcgga aattggtcga ggcatcggcg ttagcacaag ccttgaccag
    10081 gcgacgccgt catgaggctg ccctggatca aagcctaggc tctgcgcatt gcctaagcgt
    10141 cgcaccggcg ggagggggct tgccagagag gtgaaaactt caataccttg taaggcaagc
    10201 gaatcaacaa gatggcacgg tcggttgagg gaaactacgc accttcttag gtgtggccgg
    10261 ccctactggg ggacaattgg gaggcgctag accatgatga gcttgccttt tgttctcttc
    10321 gcctgcgcgg ttggtgcggc gtggatgcgc caacgcggcg tgtccatggg gttttgggct
    10381 cttgggctca tcgccatgct cgtgctgttc cgcttccacg tcacggaagt cttgatgatc
    10441 cagctctgag gcgaaggagg gggagaacgt gtctgcggac ctatcgaagc ttctgaacgc
    10501 catcgggctg cttgccatca gcacggtgct ggcgcttgcg ctcgtcgacc agctttggtt
    10561 cggcgacttg ccatgtccct tatgcattct gcagcgtgcc ggtttcttcg ctgccgggtt
    10621 cggcattgcg ctgaatctga ttttcgggcc gaagccaagt cactatggcg tcgccatcgt
    10681 cggcgcgatt gccggcggtt ccatatcgct gcgccagatt gtgctgcata tcattccggg
    10741 caccggtcat tacggcaacg acgtattcgg cttacatctt tatacgtggg ctttcattgc
    10801 cttcgccgcg atcattctgg gttgtgcgat catgttgctc gacgaccggc agttttcccg
    10861 ggccgagccc atgtcggtgc ggttgaaggt cttgccgctt tcagcgctgc tgctgttcct
    10921 gacgcttgcg atcgccaata tcgcctcgac cgtcgcactc tgtggcgccg gcctctgccc
    10981 ggacgatccg aagacctaca tgctcttgca gggcggctag cgcactttag gagttcgcga
    11041 gacctctcaa acggtccagc gcgccttgga ggatgtaggc ggccgccatg cggtcgatca
    11101 cttcggcccg gcgctttcgc gatgcgtcgg cctcgatcaa gtgccgctcc acggctgctg
    11161 tcgacagacg ctcatcccag aatgtcatgg ggataggaac atgcgccgcc agattgcgcg
    11221 cgaaggcgcg ggttgattgg gcgcgcggac cttcgctgcc gtcgagattg agcggaaggc
    11281 cgatcacgag cccgccgacg ccttcctttt cagcgatctc ggcgatctct ttggcatcgg
    11341 ccgtgaactt ggtgcgtctc agagttttgt acggggtggc gacggtgcgg gtcacgtccg
    11401 tcagggccag ccccacggtc ttcgtgccta catccagccc gagcagcctg gcaccgggct
    11461 gtagcgccga cggcaagtct tcgataggaa ggggcgaagt gtctgtcaca gggccaagtg
    11521 aagggtaaga cgatgaaatt gacgtggttc ggccattcct gctttcgcgt ggagaccggc
    11581 gatagccgta tcctgatcga tccgtttctg agcggcaatc ccaccttcga gaaagccaac
    11641 attccctgga ccgacgtgac cgagggggtg acccatgtgg cgctgaccca cggccatgag
    11701 gatcatgtcg gcgatacggt ccgggtttgc aaggaaaccg gcaccacgct tcttgccgtg
    11761 cacgagcttg cgacctacct cgcgggccaa ggggtggaga agaccgaccc cggcaatact
    11821 ggcggcatga tcccccaagg ggacttcgat ctgaccttcg tgcaggccct gcattcgtcc
    11881 tccaacatgg tcgatgggcg gccggtctat ctcggcaatc cgtgcgggct ggtgatcgcc
    11941 ggaaaagggg acaagaccct ctatcacctg ggcgatacgg acatcttcgc cgacatggcg
    12001 ctgatcaacg agttgcacca accgcaaatc ggtatcgtac cgatcggcga ccgctatacc
    12061 atgggcgcca agggcgccgc gcttgcctgc aagcgcttct tcgacttcga gaccgtggtg
    12121 ccgtgtcact acggaacctt cccggtcatc gatcagacgg cggacaaatt tgtcgcggaa
    12181 atgggtggga accgggtcct ggtcccggag gtcggcgtac ccttcgaggt ctgaggctag
    12241 ggcgcggcct ggcaccaagt cgccgccacc acgttgccgc tccctcaggg cggtgctaca
    12301 ggaagtccca gattttcaac ccccgcgaga cccccacaaa tccatgtccg tcgacagtgc
    12361 caccgttcgc cgtattgccc gccttgcccg catcgccatt accgatgagg aggccgcgcg
    12421 gctggagacc gaactctccg gcatcctcga ttgggtggcc cagctcgacg agctcgacac
    12481 cagcaatgtg gagccgatga cgcgggttga ggctatgtcc atgaagaagc gtaaggacga
    12541 ggtgacggac ggcttctgcg ccgacgacat cgtcaagaat gcgccagagg tggacgatca
    12601 ttttttcgtc gtgccgaaga tcgtcgagta gacaaccgtg acagacctga cagacctgac
    12661 gattgcggac gcgcgcgacg ccttgcgcga caagtccttc tccgccgaag agctggcaca
    12721 agcgcatctc gatgcggtcg atgccgccaa cgcggggatc aacgcctatg tcttgccgac
    12781 gccccagcgc gcgcttgaga tggcgaaggc gagcgatgcg cggttggcca agggggaggg
    12841 cggtgcactc gaaggcattc cgctcggcat caaggatctg ttctgcacgc gcgacgtccg
    12901 caccacggcc tgctcgcata tcctcgacgg cttcacgccc ccttacgagt ccacggtcac
    12961 cgccaatctg tgggggcaag gcgcggtgat gctcggcaag ctcaacaacg atgagttcgc
    13021 catggggtcg tccaatgaga cgagctatta cggcgacgtc gtcaatcctt ggcggcgcga
    13081 aggctccgac gccaagctcg tgccgggcgg atcgtccggc gggtcctcgg cggcagtcgc
    13141 cgcgcggctg tgcttaggtg caacagcaac ggataccggt gggtcaatcc ggcaaccagc
    13201 ggcttttacg ggaactgtcg gtctcaagcc cacctacggc cgctgctcgc gttggggcat
    13261 cgtggcgttt gcctcctcgc tcgatcaggc cggaccgatc acgcgctcgg tgcgcgatgc
    13321 agccattatg ttgaatgtca tggcgagcca tgatcccaag gacacgacga gtgcggacgt
    13381 gcccgtgccc gacttcgagg ccgcggtggg caaaggtgtc aaaggtctga cgatcggtat
    13441 tcccaaggag taccgcgtgg agggtatggc gccggaggtc gaagcgctgt ggcagcaggg
    13501 cattgcctgg ctgaaggacg cgggcgcaaa catcaaagag gtgagcctgc cgcacaccaa
    13561 atacgcgttg ccgtcttatt atattgtcgc gccggcggag gcgtcgtcca atctcgcccg
    13621 ctatgacggt gtgcgttatg gcttgcgcga gacgggcagc gacgtgacct ctatgtacga
    13681 gaacacgcgc gaagcaggct ttggcgccga agtgaagcgg cgcatcctga tcggcaccta
    13741 cgtgctgtcg gccggctatt acgatgccta ttacctgaag gcgcagaagg tccgcacctt
    13801 gatcaaacgc gatttcgatc aagcgttcga gggcgtcgat gcaatcctga cgccgaccac
    13861 gcctagtcct gcttttggcg tgggcgaaaa gtccggcgat ccgcttgaga tgtatctgaa
    13921 cgacatcttc acggtgacgg tgaacatggc cggactgcct ggcgtgtcga tcccggccgg
    13981 gctgtcgtcc gaaggcacgc cgcttggcct ccaactgatt ggccgcgcgt tcgacgagga
    14041 gacgctattc cgcgccggac aggtgatcga ggacgcggca ggcccgatgg ccaagccgca
    14101 agactggtgg agcaaggggg cgtaatggcc acacgcgatg cgcagaaatg cgacaacatg
    14161 gatgaggtgc ggctcgagat cgaccgcatc gaccaggcga tggtggacct gatctgcgag
    14221 cggttcgctt ttgtggatcg cgcctggcag ctcaagaact cgcctgcgga cgcccgcgtg
    14281 ccctggcgca tccagcaggt catcgacagg gtgcgtgcgc gcgccgagga aaacaatctg
    14341 ccgccggaac ttgttgaggc tctgtggcgt caaatgatcg gctggttcat ccagtacgaa
    14401 gaagagaagc ttcgcaagcc ggggcccaag gagtagcggc ggcccatgaa cgccaagagc
    14461 gaaaaatcga acctgatcga aggcgacacc ggcgactggg aggtggtgat cggcatggag
    14521 gtccatgccc aggtcagctc caacgccaag ctgttctccg gcgcctcgac cgagtttggc
    14581 ggtgatccga actcacatgt gagcctggtc gatgcggcga tgcccggcat gctgcccgta
    14641 atcaacgagg cgtgcgtgtc gcaggcagtg cgcacggggt tgggtttgaa ggctgcgatc
    14701 aatctttatt cggtgttcga ccggaagaac tatttctatc ccgatctgcc gcagggctat
    14761 cagatcagtc agttcaagaa cccgatcgtg ggcgagggcg aggtcatcgt cgatctcttg
    14821 agcggcgagc gggtgatcgt cggcatcgag cggctgcatt tggagcagga tgcaggcaag
    14881 agcctgcacg atcagcatcc gcagcactcc tatgtggatc tcaaccgctc gggtgtggcg
    14941 ctgatggaga tcgtgtcgaa gcccgatttg cgctcctccg aacaggccaa ggcctatgtc
    15001 agtaagctgc gcaccatcct gcgctatctc ggcacgtgcg acggcaatat ggagcagggc
    15061 tccttgcgcg ccgatgtgaa cgtctccgtg cggtgcccgg gcgacgaact cggtacgcgt
    15121 tgcgagatca agaacgtcaa ttcgatccgt tttatcggtc aggcgatcga gtacgaggca
    15181 cgccggcaga tcgagatcat cgaggacgac ggaacaatcg atcaggaaac acggctgttc
    15241 gatgccaaga cgggcgagac gcggtccatg cgctccaagg aggaggcaca cgattaccgc
    15301 tacttcccgg atccggatct tctgccgctc gagctcagcc aagacttcgt ggatggcctc
    15361 gctgcggacc tcccggagct tcccgaccaa aagcgcgcac gcttcattgc cgactatggg
    15421 ctcacgcctt acgacgccga cgtgcttgtc gccgagcgcg aaagtgtcga ctatttcgag
    15481 gcggtggcga agggccgcga cgccaagcag gcggcgaact gggtcatcaa cgagttgttc
    15541 ggccgtctca acaaagaagg cctgaccatc ttcgacagtc ctgtctcggc ggcgcagctt
    15601 ggcggcatcc ttgatctcat caaagaagac acgatctccg gcaagatcgc gaaggatgtc
    15661 ttcgacattg tctggaccga cggcggcgac ccgagcgaga tcgtcgaggc gcgcgggcta
    15721 aagcaagtca ccgacaccgg cgccattgag aaggccgtgg acgaggtcat cgcggcgaac
    15781 cccgacaagg tcgagcaggc caaggagaaa cctgcgttga tcggctggtt cgtcggacag
    15841 gtgatgaagg ccacgggcgg caaggcgagc ccgcaggcgg tgaacgcact actcaagtca
    15901 aagcttggcg tcgactaggt gatgcacgag gcatgaccca cgcaattcgc gtccatgagt
    15961 atggcggtcc cgagaccctg acctgggaag aggtcgaggt cggtgaaccg ggccctggcg
    16021 aaatccgcgt caagcagact gccatcggcc tcaactatat cgacgtctat gtgcgcaccg
    16081 gcctctaccc gcaggatgcg ttgccgttcg taccgggcat ggaaggtgcc ggtgtggtga
    16141 cggcgctcgg cgaaggcgta cgcgacctga aggtcgggcg ccgcgttgcc tatgcgggcc
    16201 ctatcggggg atacgcggag gagcggttga tcgcggcgga ccgcgtggtg cgtgttccgc
    16261 aaggcgtgag cgatgagacg gccgcggcca tcatgctgaa gggcatgacc gcgcaatatc
    16321 tcctgcgccg cacctacaag gtcgggccgg atacgacgct gctgtttcat gcggcagcgg
    16381 gcggtgtcgg catgattgcg tgccagtggg cggccgcgct cggcgctaag gtgatcggca
    16441 cggtggggtc cgccggcaag gcgctgatcg cgcggagcca cggctgcacg catgtcatta
    16501 actaccgcga ggaagacgtg gtcgccaagg tgcaggcgta caccaagggt gagggcgtcg
    16561 acgtggtgta cgactcggtc ggcaaggata cgtttccggc atcgctcgat tgcctcaagc
    16621 ggcggggcat gtgggtgagc ttcggtcagg cgtcggggcc ggtgccggag tttcgcatca
    16681 accttctcgc gcaaaagggc tcgctgttcg ccacgcggcc ggccctcggc gactatatcg
    16741 ccacgcgaaa agagctggtc tcatgcgcga acgacctgtt cggggctatt gccaagaaca
    16801 aggtcaagat cgccatcgat cagacctacc cgttgaggga ggcgacgcgc gcccacggcg
    16861 atctcgaagg gcgcctcacc acagggtcga cgcttcttat tccttgattt tttcaagggg
    16921 aattcgcgcg cgaagtttat gcgtggttat cggtccgtga ccgggaatgg tcaaagaaac
    16981 cttgccaaac gccgatgtta agctttgacc cgcttttgcc ctgcgcaaga gcccgcgccg
    17041 taaaccaaag ttaccgcgcg cttcagactt cctgatttct cgttcaccac atcgattcag
    17101 acgttgttga aggacctggc ccatgatcct tcccgtgttc gacgggaatg gcgcgctgaa
    17161 catcgcgggg aaaagcgagt tcgcggcgcg gaaaaataag ggggacgtta aaatggcacg
    17221 actggaactt tcgacggcga ttaccggtca catggagttc gcagcgcaag caggtgcgcc
    17281 ggatgctttg ttcgaactcg gcatgctcta tgcgaccggc cgcgatatcg atatggatct
    17341 cgtgagcgcg cataaatggt tcaacctcgc cgcggcgcgg ggcaacgttt cggcaaagac
    17401 ctatcgtgtg gagctctccc aggagatgac cacggatcag atctccgagg cacaaaaaca
    17461 agcccgcgag tggctgcaga ctcactaagt aagccgacac acaataaaaa acgctctagg
    17521 gaggaggggg cgctggattt attccagtgc ccctttgtct gtcgagccgc aattgcggaa
    17581 gaaggagcgc atgcagcgat agctccggcg cgcaagactt tcgctctccg cgcccgcact
    17641 tcgggtcgtc ttcggcggcg gagcaggggc tcgtgccgcc ggctccgacg agccgatacc
    17701 gatcgccgag cccgcattgc gtaggccctt tttcgaggca tcgaccgccc ggccacccag
    17761 ctcgaacagc gatgtcggtt cgtcgccttc cggttccttg aaagtgcgct ggtcccgttc
    17821 gatccgctgt tggagttcgc gcacgcgcgg atcggaggtc acgttctcgg gaatggccgg
    17881 gccgtcataa gtgtaggcct tgaccagccc cacgcaggcc gccgttgcct tctccgcgga
    17941 gcaggaggcg tggttgcctt ccttggcggt gcggatcacg cagtagagct tcagcgcgtc
    18001 gctcgtcttg acgccgctgc cggtcacgcg gcagcgatac gtctcgctgg ggctcttgca
    18061 ggtcacgcag taatcggcgc cggcggctgg cgccggcagc gcaagccaca tcgttgccgc
    18121 cgcggtcagc cccgccaaga caaacggccc tcgatggaaa ggtccacgca tggcgctcac
    18181 gcccttctgg tgcgtcatgg ccgcgattct agcatattgg cgggggtcgc aatacacgct
    18241 ccgaagcccg tcccaggccc tgccagggcc gattccggcc ggatctcgcg accttcctcg
    18301 attcttgcgg cttggctccc catttcccgg gggcggcttt ctactggaaa aacgcacgac
    18361 gcgacattcc gttcgtaggg gaaggtgccg tgttgaccgg ccaacgccgg atcagtaagg
    18421 ctttgcgtca acggagacgt ggccgagagg ctgaaggcgg cggtttgcta aaccgttata
    18481 cggtcaaaag ccgtatcggg ggttcgaatc cccccgtctc cgccaacttg ggctcccggc
    18541 caggcctggg cccagagggc gaaaacatga cccctgatcc cctgaagtcc gatcctgaac
    18601 aggacctcga tcgcgcgttc atcgagcggg agcgcaaccg ctttgctggc cgggcgctct
    18661 cattcctggt cctgctgaac ggcgccgccg cgctcattct gctcgccatc atggcgcaag
    18721 ccccggaatc gaccgtcgat ggaaaggtcg ccacggccct gctgttcttc agcagcggag
    18781 cgatggcagc cttgctcgcc gcgtttttgg cctacatcaa tcgaacagtc aggatgcggc
    18841 aatcggcgcg acgggaagaa cttagggctg cgttgcgcgg gctcgctatc gccgcggtga
    18901 tcggcagcgg ggcggcgttc ctcgtcggca tgaatatggt ggcgatgagt gcagtcgaga
    18961 aatcgagttc acaccccaag ggccaacgcg aggacaagcc ggccaagtct gcgccaatcg
    19021 agaaggtgga tgatcgaggc ggggcccagg atcaggccac gcgtttcggg tagggcggtc
    19081 agcctctact cggggccggt catctcgcgg gtgaagtccg cccacgcctt ttgcccctcg
    19141 gcggtcagcg cgtccacctt ttcgctgacg acgtcccagt tgacgatacg cttctcctcc
    19201 gtgccgccgc cgtcccggtc caagacgtag accgcgatga tcgtgagcgc aacgccgagg
    19261 atgaatccaa caagaaaacg catagaaacc ccaccactgt gcccctagat cataggtcgc
    19321 taggggcctg agaggaagcg ggttcgctag cccgttggcc taaagtctga gggacatcaa
    19381 cgcctggcag cgcgagagtt tgatcttggt caaggtccga ctcgtctgcc tggcgcaatt
    19441 ctgcccaaaa tgaaggggaa accgcatggc ggcactgctc gatcgagacg agtggagggc
    19501 attttcggat tttctggcgc tggggggcgc ggccctttgc gtcatccttg gcctcgtcct
    19561 agcactggcc ggcgaagacc cggtcatcgc cttccacggt tgcgttctgc tgggcgccgc
    19621 ggcattggcc tttttcttta tcctgacgca gatcatggag aagaaagaga cccatgacgc
    19681 gacgaactat gcggacgggg tggttcgcgc cg
//
LOCUS       DBNHVU010000114         9755 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1901154, whole genome shotgun sequence.
ACCESSION   DBNHVU010000114 DBNHVU010000000
VERSION     DBNHVU010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 9755)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9755)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9755
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1901154"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="AC16Y7_04455"
     CDS             <1..967
                     /locus_tag="AC16Y7_04455"
                     /inference="COORDINATES: protein motif:HMM:NF014686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR5708665.1"
                     /translation="GPIADGFQWDHLEPALAELTHNAPSPVCGLIYGAGFEDRAHLLP
                     YINERWPLLGNDARTVTRIKTPETFFAALDALGIAHPATQTERPANRTGWVAKRRGGA
                     GGSHIGQDADASDVYYQELVSGRTVSALFVANGQNVRVLGLSEQWTAPAPGKPWRYGG
                     AVCPATVPDSTAAQMERATLGVAKVFGLKGLASADFVLTETEIYLLEINPRPGATLDI
                     YACANQPPLALHLDALANRALPENQPAIEGAAAAAIVFAPETLVIPHDTAWPTWTADR
                     PKPGERIDKQRPICTVLARAGDEAEARNLAETRQASILAKIQNTH"
     gene            1027..1992
                     /gene="mch"
                     /locus_tag="AC16Y7_04460"
     CDS             1027..1992
                     /gene="mch"
                     /locus_tag="AC16Y7_04460"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HIR5708666.1"
                     /translation="MRLPSINAQVAPLVRKIIDNAEALRVAVTKGSLGETLIDCGVDV
                     VGGLEAGRLLSEVCLGGLGQVTFQTIDTDKPWPFMVTIHTSQPVLACLGSQYAGWSLS
                     ADVDGKKFNVLGSGPARAIGSSEKLFDELGYRDKADSATIVIEADKAPPAALVEEIAK
                     ACKIKPAQLTILFAPTSSLAGTVQIAARCLEVALHKAHELHYPLADIVDGIATVPVPP
                     PTPGFIEAMGRTNDAIIYAGCVQLFVTGDADAAKKLANELPSANSKDYGKPFAETFAA
                     VKGDFYQIDPMLFSPAKVIVSSLDTGESFHSGKIDDAVLQRSFTT"
     gene            1999..2934
                     /locus_tag="AC16Y7_04465"
     CDS             1999..2934
                     /locus_tag="AC16Y7_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR5708667.1"
                     /translation="MTAKTTTNPSIVLFGDGRDWHGRSLLRAFKRRGIKPVVAPLARC
                     GFSTETATGLAILGLGDHRPKGAFVRFIPGGSFEQVTLYLGLLHGLRELGVTVWNDAR
                     AIERCVDKSTTTFFLQKAGLPTPRTFAGVDRANAEEIVTRLAAEGHRLVQKPLFGAQG
                     EGLRLIESPADLSPPEEVEGTYYLQEFVPTPTDRYQDWRLFVCAGQVISAMIRHGPSW
                     ITNIKQGARAKAAIPSQDLTELALRAAACVGADYAGVDIIQAPDGQFLVLEVNSMPAW
                     NGLQRVTRSGISDQVVDAFLAACLPPALSHAEEEA"
     gene            2931..3779
                     /locus_tag="AC16Y7_04470"
     CDS             2931..3779
                     /locus_tag="AC16Y7_04470"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HIR5708668.1"
                     /translation="MTSGLSPEKVSQLFLDACLAELDALKPGNVHRFADGHGMSVADF
                     EKSAAAAAPAMGDAGLDLGARIEQAVAATQVAVGQNTNLGIILLAAPLAQAALAPLPG
                     NLHARLRAVLDSLTVEDARAAYRAIRTAEPGGLGNAPAHDVAGEPDVTLLEAMSAAET
                     KDRIAWNYTHGFVDIFDLGLKWLEGGRRRWGSVHWVTTYVYLGFLAHIPDTLIERKFG
                     SEAASEVRDEARPIEAGLSQCAAPQDMAAPLHAFDASLKERGLNPGTSADLTVATLFA
                     AALQGL"
     gene            3973..4482
                     /gene="fae"
                     /locus_tag="AC16Y7_04475"
     CDS             3973..4482
                     /gene="fae"
                     /locus_tag="AC16Y7_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HIR5708669.1"
                     /translation="MAKITGVSVGESLVGDGNEVAHIDLLIGPRGSAVETAFCNALTN
                     NKDGFTTLLAVVAPNLLCKPATILYNKVTIKNATQAVQMFGPAQYAVAKAVADSVAEG
                     VIPANEADDLYVCVGVFIHWEAKDDKKIQDFNYTATKEAIARAVAGEPKASDVTAKKD
                     SAAHPFAAG"
     gene            complement(4590..5372)
                     /locus_tag="AC16Y7_04480"
     CDS             complement(4590..5372)
                     /locus_tag="AC16Y7_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013168.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HIR5708670.1"
                     /translation="MTLQSELTTATLAQDFAVIPVLDLKDGLVVHARAGKHAHAGKRA
                     EYRPIATPFGPPHDGAAIARALLGITFSPALYIADLDAILGRGRNLDMCRHLGQALPG
                     TELWIDAGFAQVGDCAAWLPLGATLVVGSETIADLAAWEDLRAAFGQNVVLSLDFDEQ
                     GLRGPERLLADPALWPKRVILMSLGRVGTETGPDVARLEETLQRADGREVYVAGGVAG
                     LDDLAAVEKRGARGALAATALHSGALGQKEIAALVRERRSQV"
     gene            5426..6442
                     /locus_tag="AC16Y7_04485"
     CDS             5426..6442
                     /locus_tag="AC16Y7_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR5708671.1"
                     /translation="MRASLGWDLGGANLKLARAEDGRVVSVAQIPCPPIADPEKFNQA
                     LNEALETAPTNGRHAVTMTGELSDVFPSRAAGVAYLTGLMQKTTGADTLFYSLRDGLI
                     GADQVRDAWQTVASANWHASATLAAKQQEDGLFVDVGTTTTDLIPFKDGSANAVGKTD
                     GERMTEGELIYAGVVRTPVMAIAQEAPFMGRLQGIAGERFATMADVYRLTGELPVDAD
                     PYETTDGRGKDLDESATRLARMLGRDADEADFIAWKALAHFLGRRQLDRIEADAKVLI
                     ARESLRAEAPVIGAGCGRFVAQHLAKRLDRPYRDFAEIIDCADDAREMAATCAPAVAV
                     ALLV"
     gene            6565..7743
                     /locus_tag="AC16Y7_04490"
     CDS             6565..7743
                     /locus_tag="AC16Y7_04490"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006636 - unsaturated fatty acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HIR5708672.1"
                     /translation="MPSDSGPIDHHEDIVYPSAIPFVLVHLACFGAIWSGVTWESLIL
                     CVTLYWLRIFAIGAGYHRLFSHRAFSTSRAFRFVLAVLAQSTSQKSVLWWASKHRHHH
                     LHSDTELDVHSPRHTGFLYSHLGWIFARRHDTFDEDSVADLMRYPELRWLHKFELLPA
                     ALLALLCFLIAGWPGLFVGFFWSTVLVYHGTFCINSLAHVSGRKRYVTGDDSRNNWLL
                     AIFTMGEGWHNNHHAFQSSVRQGFRWYEWDPTYYILKALSWTGLVWDLRMPPKQVVRN
                     EHGLGASVINRAAEQLAGHFNSEKIASSIATALHRPELAALREQLARAQDRSADVLAA
                     MHLPQVPHMPSRDELAAKARTMFAKTKSLEEIADRAYEVLLTSVGAKLMAGAESQPSA
                     "
     gene            7831..8193
                     /locus_tag="AC16Y7_04495"
     CDS             7831..8193
                     /locus_tag="AC16Y7_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708673.1"
                     /translation="MRILPLCATALIASGLVLGLCAHDAQASPLPAMTSAMTSFSADM
                     TAVEKVGWRRRYYLRHGVWPTRGRNAYDEETGVVAAPGEPIILAPVRPRSCGQYRFWN
                     GYACVDARFNDPDLGFVE"
     gene            8212..8724
                     /locus_tag="AC16Y7_04500"
     CDS             8212..8724
                     /locus_tag="AC16Y7_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HIR5708674.1"
                     /translation="MMAYVALLRAVNVGGTGKLPMSELVAMCEGLGFSSVKTYIASGN
                     VVFSTRAREGTVKAKLEEALHNYAGKPVGVMVRTAAEMAAALAHNPFPNAAPNRTLAI
                     FLDAPPPDDTLDTLSGRKNEEVALGVREVYVNYGDGMGTSKLKIPAAKTGTARNMNTI
                     AKLAAMAAAI"
     gene            complement(8762..9319)
                     /locus_tag="AC16Y7_04505"
     CDS             complement(8762..9319)
                     /locus_tag="AC16Y7_04505"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR5708675.1"
                     /translation="MQQTVTMNGLDVQAATETVAALKADASLAKFQFRARNVWVDGGE
                     NRSTIRGFYGVGREDDSRTADFEFVNGEPPVLLGKNEGANPVEFLLHGLAGCVTTTLI
                     LHATMRGITIEKLSTELEGDIDLYGLLGLDPEVSPGYREIRIRMHIEADCTDEELDDL
                     LAAAKAHSPVCNTVCRPVPVVLNRA"
     gene            9455..>9755
                     /locus_tag="AC16Y7_04510"
     CDS             9455..>9755
                     /locus_tag="AC16Y7_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase family
                     protein"
                     /protein_id="HIR5708676.1"
                     /translation="MPQETEAIDALIVAVPETAGSALYGMVDVLAAAGSLWQALAGAE
                     PDARSIRPRIVSPLTKPFVCGNRVPVAPDLDIADDPQAEIVILPELWLAPDDDFKG"
BASE COUNT         1793 a         3141 c         3085 g         1736 t
ORIGIN      
        1 agggccgatc gcagacggct tccaatggga tcacctggaa ccagcgctgg cggaactaac
       61 gcacaatgcc ccctcgcccg tgtgcggact gatctatggc gcgggcttcg aagaccgcgc
      121 gcatctcctg ccatatatca acgagcgttg gccgctgctc ggcaatgacg cacgcaccgt
      181 cacacgaatc aaaacgccgg agacgttctt cgcagcactc gatgcgctcg gcatcgctca
      241 tcctgcgacg cagacggagc gccccgcaaa ccgcaccggc tgggtcgcca aacgccgtgg
      301 cggcgctggc ggcagccata ttggccagga tgccgacgca agcgacgtct actaccagga
      361 gttggtgagc gggcgcaccg tctcggccct cttcgtcgcc aacggacaaa acgtgcgcgt
      421 cctcggcctt agcgagcaat ggacggcgcc tgctcccgga aagccttggc gctatggcgg
      481 ggcggtgtgt ccggcgacgg tgccggacag cactgccgcg cagatggagc gcgccacgct
      541 cggcgtggca aaggtttttg gcctcaaagg gctcgcctcc gccgacttcg tgctgacgga
      601 aaccgaaatc tacctgctgg aaatcaaccc gcgccccggc gcaacgctgg atatctacgc
      661 ctgcgccaat cagccgccgc tcgcccttca cctcgatgcg ctagccaatc gcgcgcttcc
      721 cgaaaatcaa cctgcgattg agggggcagc ggcggcggcg atcgtcttcg cgccggaaac
      781 gcttgtcata ccgcacgaca cggcgtggcc cacttggacc gccgaccggc caaaacccgg
      841 tgagcggatc gacaaacagc gcccgatatg cactgtcttg gcgcgcgctg gggacgaggc
      901 ggaggcaaga aacctcgccg agacgcggca agccagcata ctggccaaga tacaaaacac
      961 acactaagcg ttgaggggga ggctgagttg agcaagaaca agaacaaaaa aagcacaaaa
     1021 caagagatga gactgccgag tatcaatgcc caagtcgcac cgctggtgcg caagattatc
     1081 gacaatgccg aagcactgcg tgtcgcagtg accaaaggct cgctgggcga aacgctgatc
     1141 gattgcggcg tggacgtcgt cggtggactg gaagccggtc ggcttctttc ggaagtatgc
     1201 cttggtgggc ttggtcaggt gacgttccaa acgatcgaca ccgacaagcc ttggccattc
     1261 atggtgacca tccacaccag ccagccggtg ctcgcctgtc tcggctcgca atatgcgggc
     1321 tggagtctgt ccgccgacgt agatggcaag aagttcaacg tgctcggctc tgggccggcc
     1381 cgtgccatcg gctcctcgga gaagctgttc gacgaactcg gctatcgcga caaggccgac
     1441 agcgccacga tcgtcatcga ggcggacaag gcgccgcccg cggcgctcgt cgaggagatt
     1501 gccaaggcct gcaagatcaa accggcgcaa ctgacgatcc tgtttgcgcc gacgtcgagc
     1561 cttgccggca ccgtgcagat cgccgcgcgc tgtctcgagg tcgcgctgca taaagcgcac
     1621 gagctgcact acccgcttgc ggacattgtc gacgggatcg ccaccgtgcc cgtgccgccg
     1681 ccaacgcccg gcttcatcga ggccatgggg cgcaccaatg acgccatcat ctatgccggt
     1741 tgcgtccagc tttttgtgac tggcgatgcc gacgcggcca agaaactcgc caatgaactg
     1801 ccgagcgcca attcgaagga ctacggcaag cccttcgccg aaacgtttgc cgcagtgaaa
     1861 ggcgactttt atcaaatcga tccgatgctg ttcagccccg ccaaggtcat tgtgagttcg
     1921 cttgacaccg gcgagagctt ccacagcggc aagatcgacg acgccgtgtt gcagcgctcc
     1981 ttcaccacct gatcgctaat gacagccaag acgaccacca atccgtccat cgtcctgttc
     2041 ggcgacggac gcgattggca tgggcggagc ctgttacgcg ccttcaagcg ccggggcatc
     2101 aagccggtcg tcgcgccgct ggcgcgttgc ggcttctcca cggagaccgc gacgggactc
     2161 gccattctgg gccttggcga ccatcggcct aaaggcgcct ttgtgcgttt cattcccggc
     2221 ggcagtttcg agcaggtgac gctttatctc ggcctgctgc atggcttgcg cgagctcggc
     2281 gtcacggtgt ggaacgatgc gcgcgccatc gaacgctgcg tggacaagtc gaccaccacc
     2341 ttcttcttgc aaaaggcggg gctgccgacg ccgcgcacct tcgctggcgt tgatcgcgcg
     2401 aatgcagaag agatcgtcac gcgccttgcc gcggagggtc accggctggt gcagaagccg
     2461 ctgtttggtg cgcaaggcga aggcttgcgg ctgattgaga gccccgcgga tctgtcgccg
     2521 cccgaagagg tggagggcac atactacctg caagagttcg tgccgacgcc gacggatcgc
     2581 tatcaggatt ggcggctctt tgtctgcgcg gggcaggtca ttagcgctat gatccgtcat
     2641 gggcccagtt ggatcacgaa catcaagcaa ggcgcgcggg ctaaggcggc gatcccgagc
     2701 caggacctta ccgaacttgc gttgcgtgcc gcggcctgtg tcggcgccga ctatgccggt
     2761 gtcgacatta tccaggcgcc cgacgggcag ttcctggtac tggaggtgaa ttccatgccg
     2821 gcgtggaacg gacttcagcg tgtgacccgg tccggcatct ccgatcaggt cgtcgatgcg
     2881 tttctcgccg cgtgcttgcc tccggccctc agccacgccg aagaggaagc gtgacgtccg
     2941 gcctaagccc agagaaggtt tcccagcttt tccttgacgc ttgccttgcc gaactcgatg
     3001 cgctgaagcc gggcaatgtg caccgctttg ccgacggtca tgggatgagc gtggccgatt
     3061 tcgagaaaag cgccgccgcg gccgcgcccg cgatggggga cgccgggcta gacctgggcg
     3121 cccgcatcga gcaggcggtg gcggcgacgc aggtagccgt cggtcagaac acaaatctcg
     3181 gcatcatcct tctggccgcg ccgctggccc aggcggcact cgcccccctc cccggcaacc
     3241 tgcatgcgcg gctccgcgcc gtcttggaca gcctcacggt cgaggacgcg cgtgccgcct
     3301 atcgcgccat ccgcaccgcc gagcccggcg ggctcgggaa cgcgcccgcg cacgacgtgg
     3361 ctggcgagcc ggacgtcaca ttgctggaag ccatgagcgc ggcggagacg aaggacagaa
     3421 ttgcatggaa ctacacacat ggcttcgtgg acatcttcga cctcggactg aaatggctgg
     3481 agggcggccg acggcgctgg ggctcggtcc actgggtgac gacctacgtc tatctgggtt
     3541 tcctggccca tatccccgat acgctgatcg agcgcaaatt cgggagcgag gccgcaagtg
     3601 aggtgcgcga cgaggcacgg cccatcgagg ccggtctgtc ccaatgcgcc gcgcctcagg
     3661 acatggccgc cccgctgcac gcattcgacg cctctttaaa ggaacggggg ctgaatccgg
     3721 ggacttccgc cgatctcacc gtggcgaccc tgttcgcggc cgcgcttcag ggcctgtaaa
     3781 ccaccccctc gggaaagacc aaacggcggc aaaatctgcc aatatcggct tgcatccctg
     3841 ccgcccggcc ttatgttcac cagcgcggag ctctgactac gagttgcgac ggcgtatcgc
     3901 cgggctatgg gccggcagtg ccgttttgtt attttttaga tcacgtctca gtatttaggg
     3961 aggggttggc tcatggccaa aatcaccggt gttagcgttg gcgagtctct cgtcggcgac
     4021 ggtaacgaag ttgctcatat cgatctgttg atcggtcccc gcggtagcgc cgtcgagacg
     4081 gccttctgca acgcgctgac caacaacaag gatggtttca cgacgctgct cgcggttgtc
     4141 gcgccgaacc tgctctgcaa gccggcaacg atcctttaca ataaggtcac catcaagaac
     4201 gccactcagg ccgttcagat gtttggccct gcgcagtacg ccgtggccaa ggccgtggct
     4261 gactccgtgg ccgagggtgt gatccccgcc aacgaggcag acgatctgta tgtctgcgtt
     4321 ggcgtgttca tccattggga agccaaggac gacaagaaga tccaggactt caactacacg
     4381 gctaccaagg aagccatcgc ccgcgccgtt gccggcgagc cgaaggcctc cgacgtgacc
     4441 gccaagaagg attccgcagc ccatcctttc gccgctggct gaaaaggaga ggaccccgat
     4501 cgggatgctc ccggcggaga atgtgggccg ggcccgattc ggcggttgct gaaaaagctt
     4561 ggtttctaag acacagggct aaccgcccat cagacctgag atcgccgctc tctcacgagg
     4621 gcggcgattt ctttttggcc aagggcgccc gaatgcagcg ccgtcgcggc cagcgcgccg
     4681 cgggcgccgc gcttctccac cgcggcgaga tcgtcgaggc cggcgacgcc tccggccaca
     4741 tagacctccc ggccatcggc gcgctgtagc gtctcctcca ggcgcgccac gtcggggccc
     4801 gtctcggtcc cgacacgccc caggctcatc aatatcaccc gtttgggcca tagggccgga
     4861 tcggccagga gccgttcggg gccgcgtagg ccctgctcgt cgaaatcgag cgacaagacg
     4921 acgttctggc cgaacgccgc gcgcaagtcc tcccaggcgg cgagatcagc aatcgtctcg
     4981 ctgccgacga cgagggtggc gccaagcggc agccaggcgg cgcagtcacc cacttgcgca
     5041 aaacccgcat cgatccacaa ttccgtgccc ggaagcgctt ggccgagatg gcggcacata
     5101 tcgaggttgc ggccccggcc cagaatggca tcgagatcgg cgatatagag cgccggcgaa
     5161 aacgtgatgc ccagcagcgc ccgggcgatg gccgcgccgt cgtggggcgg cccgaatggc
     5221 gtggcgatgg gccggtattc ggcccgcttg ccggcatgtg catgcttgcc cgcgcgcgcg
     5281 tggacgacta acccatcctt gaggtcgagc acggggatga cagcgaagtc ttgtgccagc
     5341 gtggccgtgg tgagctctga ttgaagcgtc atggcggtac gggcctcttg tcgctccttt
     5401 tatcatgact tggattgagg gctgaatgag agcgtcttta gggtgggatt taggcggcgc
     5461 caatctaaag ctggcgcggg ctgaggacgg ccgggtcgtt tcggtggcgc aaatcccctg
     5521 cccgcccatc gctgatcccg aaaaattcaa ccaggccctc aacgaggcgc tggagactgc
     5581 gcccacgaac ggccggcacg cggtcaccat gacaggcgag ctgtcggacg tattcccctc
     5641 gcgggcagcg ggcgtcgcct atctcacagg gctgatgcaa aagaccactg gcgcggacac
     5701 gctgttctac agcctgcgcg atgggctgat cggcgccgat caggtgcgtg acgcctggca
     5761 gaccgtcgcc tcggccaatt ggcatgccag cgccacgctc gccgcaaagc agcaagaaga
     5821 cgggcttttc gtggatgtgg ggaccacgac gaccgacctc attcccttca aagacggcag
     5881 cgccaacgcc gtgggcaaga cggacgggga acgcatgacc gagggcgaac tgatctatgc
     5941 cggtgtggtg cggaccccgg tgatggcgat cgcgcaggaa gcgcccttca tgggacgcct
     6001 acaaggcatt gccggcgagc gttttgccac catggcggac gtctatcgct tgaccggcga
     6061 attgccggtg gacgccgacc cctatgagac gacggacggg cgaggcaagg acctggacga
     6121 aagcgcgacc cgcctggcgc gcatgctggg ccgggacgcc gacgaggcgg acttcatcgc
     6181 ctggaaggcg ctggcgcact ttctgggccg gcgccagctc gaccggatcg aggccgacgc
     6241 caaagtgctg atcgcccgcg agagcctccg cgccgaagcg cccgtcatcg gggccggctg
     6301 cggccggttc gtggcccaac acttggctaa acgcctggat cgcccctatc gggactttgc
     6361 cgagatcatc gattgcgcgg acgatgcgcg tgaaatggcc gcaacctgcg cacccgccgt
     6421 ggccgtggcg ctacttgtat agtggcgtcc tgaggcccag atggaagggg cagaacattt
     6481 ggccggccct ggcctttccc cccgaatccg acaaaaatgg cgtttgcaac gcgttgtttg
     6541 caccgagaag aggacagcca gtttatgccc agcgacagcg gccccatcga ccaccacgag
     6601 gatattgtct atccgtcggc gatcccgttc gtcctggttc acctcgcctg tttcggcgcg
     6661 atctggtcgg gcgtcacctg ggaatctctg attctatgcg tgacgctgta ctggctgcgc
     6721 atcttcgcca tcggcgccgg ctaccaccgg ctgttttcgc accgcgcctt ttccaccagc
     6781 cgggctttcc ggttcgtcct ggccgtgctg gcccagagca cctcgcaaaa aagcgtcttg
     6841 tggtgggctt ccaagcaccg ccatcaccat ttgcattccg acaccgagct cgacgtacac
     6901 tccccgcggc acacgggctt cctttacagc cacctgggat ggatcttcgc gcggcggcac
     6961 gacacgttcg acgaggatag cgtcgccgat ctcatgcgct atccggagtt gcggtggctg
     7021 cacaaattcg agctgctacc ggcggccctc ctcgcgcttc tgtgtttcct gatcgccggt
     7081 tggccgggcc tgttcgtcgg cttcttctgg agcacggtgc tcgtttatca cggcacgttc
     7141 tgcatcaact cgctggccca tgtgagcggc cgcaagcgct acgtgacggg cgatgattcc
     7201 cgcaacaact ggctcttggc catcttcacc atgggcgaag gctggcacaa caatcaccac
     7261 gcattccaat caagcgtccg gcagggcttt cgctggtatg agtgggatcc gacctattac
     7321 atcttgaagg cactgtcttg gaccggcctt gtttgggatt tgcgtatgcc cccgaagcaa
     7381 gtcgttcgaa acgagcacgg tctcggcgca agcgtgatca accgcgcggc cgaacagctc
     7441 gcaggccatt tcaactcgga gaagattgcg agctccatcg ccactgccct gcacaggccg
     7501 gaacttgcgg ccctgcgaga gcagctcgcc cgcgcccaag accgctcggc ggacgtcttg
     7561 gcggccatgc atctcccgca agttccgcac atgccgagcc gcgatgagct tgcagccaag
     7621 gcgcgcacga tgtttgccaa gacgaaatcc ttggaggaga tcgcggaccg ggcctatgag
     7681 gtgctactga cctcggtggg cgcgaagctc atggccggcg ccgaaagcca gccaagcgcc
     7741 taaagcctga cgcgttgccc tgacactctg atggtgcggc cgatcgtgct agaacagcac
     7801 ggcaaggaag acccaaccaa ggagcgtcac atgcgtattc tccccctttg cgccactgcc
     7861 ctgattgcta gcggtcttgt tcttggactt tgtgctcacg atgcgcaggc cagcccgctt
     7921 ccagccatga catcggcgat gacgtcgttt tcagccgata tgacagcggt tgagaaagtc
     7981 ggctggcgcc ggcgctacta cctccgtcat ggggtttggc caacacgggg ccgcaatgcg
     8041 tatgatgagg aaaccggcgt ggtggccgcg cccggagagc ccatcatcct cgccccggtt
     8101 cgtccgcgca gttgcggcca gtaccgcttc tggaacggct atgcgtgtgt ggacgcgcgc
     8161 ttcaacgacc ccgatctcgg ctttgtcgag tagattgagg ttcccgccgg catgatggcc
     8221 tatgtcgcac tcttgcgcgc cgtcaatgtc ggcggaaccg gtaagctgcc aatgtccgaa
     8281 ctcgtggcca tgtgcgaggg cctcgggttc tcgtccgtga aaacctatat cgcctcgggc
     8341 aacgtggtgt tctcaacgag ggcgcgcgaa gggaccgtca aagcaaagct cgaagaggct
     8401 ttgcacaatt acgccggcaa gcccgtgggc gtcatggtgc ggacggcggc cgagatggcg
     8461 gcggcactcg cgcacaaccc ctttccgaat gccgcgccga accgaacgct cgcgatcttt
     8521 ctcgatgcgc cgccgccgga cgatacgctg gatacgctgt cgggacggaa aaacgaagag
     8581 gtcgcgctcg gcgtccgcga agtctacgtg aactacggcg acggcatggg gacatcgaag
     8641 ctaaagatcc cggcggcgaa gaccggcacc gcacgcaaca tgaatacgat cgccaagctc
     8701 gcggcgatgg cggcagcgat ctaaggcgca gccccacgca tgggggccgc gccttgtctc
     8761 gttacgcgcg gttcagcacg accggcacgg gccggcagac cgtgttgcac acgggagagt
     8821 gggccttcgc ggccgccagc agatcgtcaa gctcttcatc cgtgcagtcc gcctcgatgt
     8881 gcatgcggat gcggatttct cggtagccgg gcgatacttc gggatcgagg ccaagcagac
     8941 cgtaaaggtc gatgtcccct tccaactcgg tggagagctt ttcgatggtg atgccgcgca
     9001 tggtcgcgtg caggatcagc gtggtggtga cgcatccggc gaggccatgc aggagaaact
     9061 cgaccgggtt ggcaccttcg ttcttgccga gcagcacggg cggctcgcca ttcacgaatt
     9121 cgaagtcggc cgtgcgggag tcgtcctcgc gtcccacgcc atagaagccg cggatcgtgg
     9181 accggttctc accgccgtcc acccagacgt tgcgcgctcg aaactggaac ttggccagac
     9241 tggcgtccgc tttcagggcc gcgaccgtct ccgtcgcagc ctgcacgtcc aaaccgttca
     9301 tcgtgactgt ctgctgcata ttcatggtct cctccttctt ggctccggcc agccgccgat
     9361 cggccggccg tctacccgag aaggtagaaa ggccatgccc cgaggctcag gtcgcttttc
     9421 gtcaaaaagt gtccattcgt gccataatcg gcccatgccc caagagaccg aggccatcga
     9481 tgctctgatc gtggccgtgc ccgaaacagc gggttcggcc ctttacggca tggtcgatgt
     9541 gctggcggcg gcgggtagcc tctggcaggc gttggctggc gccgagcccg acgcgcgctc
     9601 cataaggcca cgcattgtct cgcccctcac gaagccgttc gtctgtggca accgggtgcc
     9661 ggtagccccc gatttggaca tcgccgatga tcctcaggcg gagatcgtca tcctgccgga
     9721 actgtggctg gcgccggacg atgacttcaa agggc
//
LOCUS       DBNHVU010000115         6223 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_489179, whole genome shotgun sequence.
ACCESSION   DBNHVU010000115 DBNHVU010000000
VERSION     DBNHVU010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_489179"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..4637
                     /locus_tag="AC16Y7_04515"
     CDS             6..4637
                     /locus_tag="AC16Y7_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5708677.1"
                     /translation="MFESGDGDAAPCYVGSVKANVGHLEAVSGLAGLLKAMLCLEKNE
                     IPGQANLSKPMKVAHAEDARVVVPSETTPWPRGPSPRRAGVSCLGLGGSNSHVVIEEP
                     PAPIEQAVGADRSVHILKLSAKKPEQIARMASALQQALAGQPDLRLGDICYSANFGRA
                     DFEERAAIVAHSRQDLALTLSSLAEGSSSDKIFRGRAQKGGPKVALLLCGSAASYRGM
                     GRDLYDHEPRFAALIDEVDQALRKATGTSLYGLIYEKGESDGTIIEDTSLARAALFAT
                     QYALANFLIEVGLKPEMIIGTGVGDIVSSVLDKKLSLADGLKAALAGNAEQTIEKLQK
                     ALDDALAAGVDMIVEAGSQSALLAEIGAASGTPLMSCLFQGKDDCEQLAVVLGSLYVI
                     GVDVDWQALDAPFSRRRLALPTYCFDRTRFWFTDGAPVPGPRTAPPAKVAKPSETVRS
                     EAPVPEPAPDRAPAQTPAQEPPAASAISTITRQELFELLRRNIAQATSMMADDIGEGT
                     KFDEFTIDSIAAFELGLRLEKQLGVMPDPEIFSEDMTFGAAVDALHAFVTTARAKSNG
                     SAPAPKSPEPPSVSALTPYLPRVEVPVPVAGKRHDQGNGPQTVDEQSVSDARAFLEAI
                     KQRADPTDYLETIRPDFTAALHEMLLDVDFDSAVGDRLSGSRFGVPVHAIDMVGGFGS
                     TLFGHNHPALVGVLREALAAHRPKFVQMANRTAAGTLAKDLVSRVAALTGRDYRVVLG
                     STGAEMVDAALKHALFEWSQRLKTFDAACNSAGIDAPEALSKPPVVLAIEGSYHGKTL
                     GAYSLTWHAPGRDALGLNGPFEVVWLPRDDSAAASEIFERYQGDIETSDGPVRFSRIA
                     GLFLEPIQGEGGIFPLSKDFAGTLRKLADAVECPIVVDEIQSGMGRAGAFTASSLIDL
                     RGDYYLFGKSLGGGLTKTTALLIDAERYAEGYGFAHTSTFAEDDHGALVALRALQLLD
                     EDDIATRARVAGKYLTDSLEEVKARYPGVLSAVRGCGLMAGFELSSQQDNASQLIRGL
                     SEQGLLGVFVASYLLHRCGVRVGTTLSRPSTLRLEPSAYISRAEMDQAVRSVEAVCQV
                     IASGNAADLVRHLAQSFRREGQTPFKLSENPVAGRTEPVHASVTAAQKQAVFLGHMIS
                     SNDIGLLDPSLLNLTERERESLAERLVDPILIESVPLLSSSGEALNFQVYTLPLTARA
                     IVKALRNGESAKIVERVNAAVNQFAEEGASVVGLGGLLSVITRNGLAVRNGFDELQIT
                     TGNTYTVALVVEAVLAAMRERDIDPATAKVGIVGAGGNIGATLVQLLSEYFPRLHLVG
                     RNASIARVERAAVGAYDAALEQQQLHGSELLTGLAAEIADFELKARLASSNGVKMNGE
                     APSAGARLRKAVIERYGTDPFLAVTGSIEDLNDCSVIVTASNSITPILTPSNVGSTTQ
                     IICDVSVPTDVDTDLVTERPDIAFIRGGVAKAPENNQFTLDVIELPKNHLLACMTETA
                     LLGFAGKQGFSAIGDIHASGVRHCHALGRKYGFSLGYVSLEKPFAIDID"
     gene            4694..6013
                     /locus_tag="AC16Y7_04520"
     CDS             4694..6013
                     /locus_tag="AC16Y7_04520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5708678.1"
                     /translation="MSSPTIPQECDVTVIGGGPTGSTAANKLASRGYQVVLIDKAAHP
                     RNTVGESLIPHFWRYTDELGVTEDIVSEGFLSKGGGLSYWGSELRRLRFRDFGHERPP
                     LHVERDIFDDILLRKAGAGGAQVFQGVQVTTIDDLEGPITTVNYRDADGQPGCLKTRY
                     VLDCSGQAAVVANQLKMRVFDEGLRFSAFWGYYRKSKYLTFDGDVRNDEEKFEHPPAT
                     LMSTVGGWGWSWHIMLRKCVSVGVIIPRERLSELRGNSPEDREELFRRHVSNIELTGR
                     LLDEDDFIPGSLRNLRNYAYKPAALTVGNCYLAGDAAAFVDPINSIGVVLAMFSGSIA
                     AHAISRSLEKPSEAQLNRELYSHQFGNRVALFRLLALPNGHDHLSQEELDRAKEAISA
                     SSDAELQLMALQATNNLRDDVLNDIFDGLGISKRPKIESVDWPALPL"
     gene            6101..>6223
                     /locus_tag="AC16Y7_04525"
     CDS             6101..>6223
                     /locus_tag="AC16Y7_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HIR5708679.1"
                     /translation="MIRSYSDHAANERTFLAWLRTGVAVIAFGFLIDRFDLFVRT"
BASE COUNT         1167 a         1806 c         1977 g         1273 t
ORIGIN      
        1 acgccgtgtt tgaaagcggg gatggtgacg cggccccatg ttacgtcggg tcggtcaagg
       61 cgaacgttgg tcacctggag gccgtcagcg ggctggcagg cttgctgaag gcgatgctgt
      121 gcctcgagaa gaacgaaatc cccggacaag caaatctcag caagccgatg aaagtcgcgc
      181 atgctgagga tgcacgtgtc gtcgtgccgt cagagacgac gccatggccg cgtggtccat
      241 cgccgcgccg tgctggcgtg tcctgcttgg ggctgggcgg ttcgaattcg catgtggtga
      301 ttgaagagcc gcctgcgccc atcgagcagg ccgttggcgc agatcggtcc gtccacattc
      361 tgaaactcag cgcgaagaag cccgaacaga ttgcgcgaat ggcctccgcg ttgcagcagg
      421 ctctcgcagg ccaacccgac ctgcgtcttg gtgacatttg ttattcggca aatttcggtc
      481 gtgccgactt tgaagagcgc gcggcaattg tcgcgcactc gcgccaggac cttgcgctga
      541 cattatcttc ccttgccgag ggcagctcaa gcgacaagat ctttagaggc cgcgcgcaaa
      601 agggcggtcc gaaggtcgca ttgctgctgt gtggaagtgc tgcctcttac cgtggaatgg
      661 ggcgcgatct ttacgatcac gagccgcggt tcgccgcact gatcgacgag gtggatcaag
      721 ccctacgtaa ggcgacggga acgtcgctct atggcctcat ttacgaaaag ggcgagtccg
      781 acgggacgat cattgaagac acctcgttgg cgcgtgcggc gctgttcgca acgcagtacg
      841 cgctcgccaa ctttctcatc gaggtcggtc ttaagccgga gatgattatt ggcaccggcg
      901 tcggcgatat tgtttcgtcg gtcttggaca agaaactgtc gcttgccgat ggcctgaagg
      961 ccgcgcttgc cggcaatgct gagcagacaa tcgaaaagct ccagaaggcg ttggacgatg
     1021 cgctcgcggc cggtgtcgat atgatcgtcg aggctggatc gcagtccgct ctactcgcgg
     1081 agattggcgc cgcaagtggt acgcctctga tgtcctgcct gttccaaggc aaggacgact
     1141 gcgagcagct ggcggtggtg ctgggctccc tctatgtcat cggcgtcgac gtcgactggc
     1201 aggcgctcga tgcacccttc agccgccggc gtttggcgct gcccacatat tgttttgacc
     1261 gcactcggtt ctggtttacc gacggcgcgc cggttccggg accccggaca gcgccgcccg
     1321 ccaaggtggc aaagccgtct gagactgtcc gttccgaggc gccggtgcca gagcctgcgc
     1381 ctgatcgagc acctgcgcaa acgcctgcgc aagaacctcc ggccgcctct gctatttcaa
     1441 cgatcacgcg gcaggagttg ttcgaactgc tccggcgtaa cattgcgcaa gcaacatcga
     1501 tgatggcgga tgacatcggc gaaggcacga agttcgatga attcaccatc gactccatcg
     1561 ccgcgtttga gttggggctg cggcttgaga agcagctcgg cgttatgccg gacccggaaa
     1621 tcttctccga ggacatgaca ttcggtgcgg ctgttgacgc actccatgcc ttcgtgacaa
     1681 ccgcacgggc gaagagtaac ggatcggcgc cggcgccgaa gtctcccgaa ccgccaagcg
     1741 tatcggcgct tacgccgtat ctgccgcgcg tcgaggttcc agtccccgtg gccggcaagc
     1801 gacacgatca gggaaacggg ccccaaaccg tcgatgagca aagcgtcagc gatgcgcgcg
     1861 cgtttctgga ggcgatcaaa cagcgcgccg atcccacaga ttaccttgag acgatacggc
     1921 ccgatttcac tgcggcgctg catgagatgt tgctggatgt ggatttcgac tcggcggttg
     1981 gcgaccggct gagcggttcg cgattcggcg tgccggtcca tgccatcgac atggttggcg
     2041 gcttcggttc tacgctgttc ggccataacc atcccgcttt ggttggtgtg ctccgggagg
     2101 ctctcgccgc gcacaggccg aaattcgtgc aaatggccaa tcgcacggcg gcgggcacgc
     2161 ttgcgaaaga cttggtctcg cgtgtcgctg ccctcacggg gcgcgactat cgggtcgtgc
     2221 ttggcagcac cggcgccgag atggtcgatg ccgcactgaa gcacgcgttg tttgagtgga
     2281 gccaacgcct caagacattt gatgcggctt gcaacagcgc cggcatcgac gcgccggaag
     2341 cccttagcaa gccgccggtc gtcctggcta tcgagggcag ctatcacggc aagacgttgg
     2401 gggcgtatag tttgacctgg catgcgccgg gccgtgatgc gctcggactg aacgggccgt
     2461 ttgaagtcgt ttggctgccg cgagacgaca gtgcggcagc ctcggagata tttgaacgct
     2521 accaggggga catcgagacg agcgacggcc ctgtccgctt cagccgcatc gccgggctat
     2581 tcttggagcc gatccagggc gaaggcggga ttttcccgct gtcaaaagac tttgccggca
     2641 cgttgcgcaa gcttgccgac gcggtcgaat gtccgatcgt cgtcgacgag attcaatcgg
     2701 gtatggggcg cgccggcgcg tttactgctt cgtcgctgat cgacttgcgc ggcgactact
     2761 atctgttcgg caagagtctc gggggcggtc tgacaaagac gacggccttg ctgatcgatg
     2821 ccgaacgcta tgccgagggt tatggcttcg cccatacctc gaccttcgcc gaggacgacc
     2881 acggcgcctt ggtggcattg cgtgccctgc agcttttgga tgaggacgat atcgcgacgc
     2941 gcgcgcgcgt cgccggtaag tatctcaccg acagtctgga agaagtgaag gcgcggtatc
     3001 ctggcgtgct atcggcagtg cgcggttgcg ggctcatggc cggatttgag ctttctagcc
     3061 agcaggacaa cgcctcgcag ctgatacgcg gactgtcgga gcaggggctt ctgggcgtgt
     3121 ttgtcgcgag ctatctgctg catcggtgcg gggttcgcgt cggaaccaca ttgtcccgtc
     3181 catcgacgct gcgcctggag ccgtccgcct acatatcgcg tgcagagatg gatcaagccg
     3241 tacggagcgt tgaggccgtc tgccaagtca tcgcatcggg caatgcagcc gatcttgtgc
     3301 gtcatctggc ccagagcttc cgcagggagg ggcagacgcc gttcaagttg agtgaaaacc
     3361 ccgtcgcggg gcggacagag cctgtccatg ccagcgtcac ggcggcccag aagcaagccg
     3421 tcttcctagg ccacatgatc agctcgaacg atatcggcct cctggatccg tcgttgctga
     3481 acctgacaga gagggagcgg gagagtcttg ccgaaaggct ggtcgacccg atcctgattg
     3541 agagcgtgcc gctgctgtcc tcgtccggcg aagccctgaa cttccaggtc tatacgctgc
     3601 cgttgacggc ccgcgcgatc gtgaaggccc tccgcaacgg cgagtctgcg aagatcgtgg
     3661 aacgggtcaa tgcggcggta aaccagtttg ccgaggaagg tgcgagcgtc gtcgggcttg
     3721 gcggattgct ctcggtgatt acgcgcaacg ggcttgcggt gcgcaatggc ttcgatgagt
     3781 tgcagatcac gacgggcaat acgtacacgg tcgctcttgt tgtagaggcc gttcttgcgg
     3841 ccatgcgcga gcgcgacatc gatcctgcga cggccaaggt tggcatcgtt ggcgcgggcg
     3901 gcaatatcgg cgctacgctc gtgcaattgc tcagcgagta tttccctcgc ctgcacctcg
     3961 tcggccggaa tgcgagcatc gcgcgggtcg aacgcgcggc cgttggagct tatgacgccg
     4021 cgctggagca gcaacagctt catgggagtg agctcttgac gggcctggcg gctgaaatcg
     4081 ccgattttga actgaaagca aggctcgcct cgtccaatgg ggtcaagatg aacggtgagg
     4141 ccccctcagc gggcgcgcgg ctgagaaagg cggtgattga gcgttatggc accgacccct
     4201 tccttgccgt gactggtagc atcgaggatc taaatgattg cagcgtgatc gtgacggctt
     4261 cgaattccat cacgccgatc ctgacgccgt caaacgtcgg ttccacgact caaatcatct
     4321 gcgacgtgtc ggtgccgacc gacgtcgata ccgatctcgt cacggagcgg cccgatatcg
     4381 ccttcatccg gggcggcgtt gccaaggcgc ctgagaacaa ccagttcacg ttggatgtta
     4441 ttgaactgcc gaaaaaccat ttgctggcgt gcatgacgga gacggccctg ctggggttcg
     4501 ccggcaaaca aggcttcagc gcaatcggtg acattcatgc cagtggcgtt cggcactgcc
     4561 atgccctggg ccgcaaatac ggcttcagtc tgggctatgt ctctctggag aaaccgttcg
     4621 ctatcgacat cgattaagca gacatgagag caaaaactct caatatcgtt ttggtgttgg
     4681 cgggaaagcg cgcatgagca gtccaactat tccacaagaa tgcgatgtca ctgtcatcgg
     4741 cggcgggccg actgggtcga cggctgcaaa taagttggcg tcgcgcggct atcaagtcgt
     4801 gcttatcgac aaggcagctc atccacgaaa cacggtcggc gagagcctaa tccctcactt
     4861 ctggcgttat acggacgagc tcggcgtcac cgaggacatc gtcagcgagg gatttttgtc
     4921 caaaggcggc gggctttcct actggggcag cgagttgcgc cggctgaggt ttcgcgactt
     4981 tgggcatgag cggccgccgc ttcatgtcga gcgcgatatc ttcgacgata tcctattgcg
     5041 caaggccgga gcgggcggcg cgcaagtgtt tcagggcgtg caggtcacga ccatcgatga
     5101 cttggaaggc cccatcacga cggtgaacta tagagacgcg gacgggcagc cgggctgtct
     5161 gaagacgcgc tatgtcctcg attgctcggg acaggcggcg gtcgtcgcca accaattgaa
     5221 gatgcgcgtc ttcgacgaag ggctgcggtt ctcggcgttt tggggatact accgcaagag
     5281 caaatacctg acttttgacg gcgacgttcg caacgatgag gaaaagtttg agcatccgcc
     5341 ggcaacgcta atgtcgaccg ttggtggctg ggggtggtcg tggcatatca tgctgcgcaa
     5401 gtgcgttagc gtgggcgtga tcatacccag ggaacgtctg tccgagttgc gcggcaactc
     5461 gccggaggat cgggaagagc tattccggcg ccatgtttcc aatattgagc tgactggccg
     5521 cttgctcgat gaagacgact tcatccctgg aagccttcgg aacttgcgca actacgccta
     5581 caagcccgct gccttgaccg tcggcaattg ctacctggcc ggcgatgctg cagccttcgt
     5641 cgacccgatc aactccattg gcgttgtcct tgccatgttc tcaggatcga tcgcggcgca
     5701 cgccatttcg cgctccttgg agaaaccgag cgaagctcag ctgaaccgcg aactctattc
     5761 gcaccagttc ggcaatagag ttgccctgtt ccgtttgctc gcgttgccca atgggcatga
     5821 tcatctctcg caagaggagc ttgatcgcgc caaggaggcc ataagtgcaa gcagcgacgc
     5881 tgaattgcag ctcatggcat tgcaggccac taacaatttg cgcgacgacg tgctcaacga
     5941 catcttcgat ggtctcggga tctccaagcg gcccaagatt gaaagcgtgg attggccggc
     6001 cttgccactc tagctgcgct tcggctcttc cagaggtctt aagccattat tggcgctttt
     6061 cggccaagct aacgctcctt tagaggatcg aggctccgcc atgatcagat cctattccga
     6121 ccacgccgcc aatgagcgga ccttcctggc gtggctgcgc accggtgttg ccgtcattgc
     6181 gttcggcttt ctcatcgacc ggttcgatct cttcgtgcgc acc
//
LOCUS       DBNHVU010000116         5295 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1528780, whole genome shotgun sequence.
ACCESSION   DBNHVU010000116 DBNHVU010000000
VERSION     DBNHVU010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1528780"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="AC16Y7_04530"
     CDS             <1..399
                     /locus_tag="AC16Y7_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5708680.1"
                     /translation="AKALPEGETGNVINLIDQKVWRLDPGYFSYTIAKSALWTATQTL
                     AQTLAPRIRVNAVAPGPVLPNRDQTNAEFEREYQSTLLKRPVSIEDVTAAIRFLLETP
                     SITGQMIALDAGQHLAWEPPMSAALERLSR"
     gene            464..2380
                     /gene="uvrC"
                     /locus_tag="AC16Y7_04535"
     CDS             464..2380
                     /gene="uvrC"
                     /locus_tag="AC16Y7_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006472446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR5708681.1"
                     /translation="MSLENPDTPASPPARARKAASGVETGAAVIARHAKTAPAGPGVY
                     RMFDAESQVLYVGKARHLKKRVQSYARGTGHNNRIARMIADTASMEFVTTKTEMEALL
                     LEANLIKRLKPRFNVVLRDDKSFPFILIARDHPMPQILKHRGARNRKGDYFGPFASAG
                     AVTRTINTLQRAFLLRSCSDTYYETRLRPCLLYQIKRCSAPCTGEITEQDYGALLDEA
                     LAFLRGESDEVKHRLHALMNEASEDQDYERAASFRDRLTALSHVQSHQGINPHTVKDA
                     DVFAVHQDAGQTCIQVFFFRSGNSWGNRAYFPRADKALGPELVLESFIAQFYDDKPPP
                     KHILLSHDIKGRRLLAEALALKAGHKITVTTPQRGEKRELVDHALVNAREALARRLAE
                     HASQAAIFARLAEVLDLDESPERIEVYDNSHISGSNPVGAMIVAGPEGFIKAQYRKFN
                     MKGEDLAPGDDYAMMREMLNRRFSRLVKERDKDQGEGWAAPDLVLIDGGPGQLSAAQG
                     VLDDLGLSDIRIAAVAKGPDRNAGRERVYQKDKAPLLLEPRDPVLYCIQTLRDEAHRF
                     AIGAHRARRKKGITASPLDEVPGIGPARKRALLKYFGSAKAVSRAGITDLEGVGGISA
                     QMAKTIYDHFHERG"
     gene            2401..3012
                     /gene="pgsA"
                     /locus_tag="AC16Y7_04540"
     CDS             2401..3012
                     /gene="pgsA"
                     /locus_tag="AC16Y7_04540"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR5708682.1"
                     /translation="MAEQGLAGGRGSNWRHFGSLPNILTYGRILAVPALVASFFVTGD
                     WGRWLAMWIFIAAGVSDFLDGYLARAWQQQSALVQMLDPIADKLIVAAALLMLAADGT
                     IAGWSLCAGVIILSREILVSGLREFLGTLNVGVPVTKLAKWKTLVQMVAIGFLLAGSA
                     GDKIWPYTTLFGLTLLWIAAILTIYTGYDYLRATFHHVVPEDE"
     gene            3012..3263
                     /gene="moaD"
                     /locus_tag="AC16Y7_04545"
     CDS             3012..3263
                     /gene="moaD"
                     /locus_tag="AC16Y7_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HIR5708683.1"
                     /translation="MRLLYFAWVKEKVGHAAEDVEVPGDVATVAELIAWLKTRGPEFA
                     HAFERAEVIRAAVDQTHARHDAKIGDAREIAFFPPVTGG"
     gene            3265..3735
                     /gene="moaE"
                     /locus_tag="AC16Y7_04550"
     CDS             3265..3735
                     /gene="moaE"
                     /locus_tag="AC16Y7_04550"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HIR5708684.1"
                     /translation="MIRIQTQDFDVGAEIAALRSGRTDVGAVVSFTGTVRDQAGQEMG
                     DVAEMTLEHYPGMTETELERIEAEANKRWPLQASLIVHRVGTLKPGNNIVLVVTASAH
                     RDAAFDAARFLMDYLKTHAPFWKRESGPSGDKWVEAKESDEAAATRWSRNNAAE"
     gene            complement(3736..4047)
                     /locus_tag="AC16Y7_04555"
     CDS             complement(3736..4047)
                     /locus_tag="AC16Y7_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708685.1"
                     /translation="MRITLVLAGLMALLALPAGDGQAGAPWLPGLNDAAPAPIAAPAK
                     AFRDCQEIARCSGCRPVYKCRSCKYQRVCNRGFCEWRDVCVWGPYQKVLPPNARIIRV
                     R"
     gene            complement(4134..4829)
                     /gene="bluB"
                     /locus_tag="AC16Y7_04560"
     CDS             complement(4134..4829)
                     /gene="bluB"
                     /locus_tag="AC16Y7_04560"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HIR5708686.1"
                     /translation="MSSLSPQVVQRTPTEEEPPKAAPAFDAAFRARLYDLLRWRRDVR
                     RFKRDALPEGMLDRLIGLACLAPSVGLSEPWRFVLVEAAGRRAAIRANFEACNKDALA
                     MQEPDRAALYARLKLAGLDEAPVQIAVFADRDTTQGSGLGRLTMTETIDYSVVMAIHT
                     LWLAARVEGLGLGWVSILDPACMAEILDVPKDWTFIGLLCVGYPQEEDDTPALERAGW
                     EHRHAPDRVVIRR"
     gene            complement(5002..>5295)
                     /locus_tag="AC16Y7_04565"
     CDS             complement(5002..>5295)
                     /locus_tag="AC16Y7_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR5708687.1"
                     /translation="KDGLIYTPTPDCFLDGITRRTVIGLAKQRGWKVIETGIMPEELA
                     DFTECFITGTAAEVMPVGSIGGYTFEVGDITRTLMNDYAALVRPAQAKVAAAG"
BASE COUNT          993 a         1798 c         1632 g          872 t
ORIGIN      
        1 gccaaggcgc tgccggaagg cgaaacaggc aatgttatca atctgatcga ccaaaaggtg
       61 tggcgcctcg accccggcta cttctcctac acgatcgcca agtcggccct gtggacggcg
      121 actcagaccc tggcgcagac cctggccccg agaatccggg tcaacgccgt cgcgccggga
      181 cccgtcctgc ccaaccggga ccaaacgaac gccgaattcg aacgcgaata ccaatccacc
      241 ctgctgaaac gcccggtgtc catcgaagac gtcacagccg cgatccggtt tctcctggaa
      301 acgccctcga tcaccggcca aatgatcgcg ctggacgccg gacagcattt ggcttgggag
      361 ccgcctatgt ccgcggctct tgaacgcctg agccgttaga ccatactttc gctgaaggtc
      421 ggcgcggacc gcctcggaga agcgccaaac ccctcgtgat cccatgtcct tagagaaccc
      481 cgacaccccc gcctcgcctc cggcgcgtgc gcgcaaggct gcgagcggtg tcgagaccgg
      541 cgccgccgtc atcgcccgcc acgctaagac ggcgcccgca ggccccggcg tctaccgcat
      601 gttcgatgct gagagccagg tgctctacgt gggcaaagcg cggcatctga agaagcgcgt
      661 ccagagctat gcgcggggca cgggccacaa caaccgcatc gcccgcatga tcgccgatac
      721 cgcgagcatg gagttcgtca ccacaaagac ggagatggag gcgctgcttc ttgaggcgaa
      781 cctcatcaag cggctgaagc cgcgcttcaa cgtagtgctg cgggacgaca agtccttccc
      841 cttcatcttg atcgcgcgcg atcaccccat gccgcagatc ctaaagcatc gcggtgcgcg
      901 gaaccgcaag ggcgactatt tcggcccctt cgcctcggcc ggcgccgtca cccgcaccat
      961 caacacattg cagcgcgcct tcctgctgcg ctcgtgctcc gatacctatt acgagacacg
     1021 cctgcggccc tgcctgctct accaaatcaa gcgatgcagc gcgccttgta ccggggagat
     1081 caccgaacag gactacggtg cactgctgga cgaggcgctt gccttcttgc gcggcgagag
     1141 cgacgaggtg aaacatcgcc tgcacgcgct gatgaacgaa gccagcgagg accaggacta
     1201 cgaacgcgcg gcgagctttc gcgatcgcct gaccgcgctg tcccatgttc aatcgcacca
     1261 gggcatcaac ccgcacacgg tgaaggatgc ggacgtcttc gccgtccatc aggacgcggg
     1321 ccagacctgc atccaggtgt tcttcttccg cagcggcaat agctggggca accgcgccta
     1381 cttcccccgc gccgacaagg cgcttggtcc cgaactggtg ctggaatcgt tcatcgccca
     1441 gttctacgac gacaagccgc cgcccaagca catcctcctg tcacatgaca tcaaggggcg
     1501 ccgcctgctc gccgaggcgc tcgcgctcaa ggccggtcac aagatcaccg tgaccacgcc
     1561 gcaacgcggc gagaagcgcg agctcgttga ccacgcgctg gtcaacgcgc gcgaggcctt
     1621 ggcacggcgt cttgccgagc acgccagcca agctgcgatc tttgcccgcc tcgccgaggt
     1681 gctggacctc gacgaaagcc cggagcgaat cgaggtctac gacaacagcc acatctccgg
     1741 ctcaaatccc gtcggcgcca tgatcgtggc gggcccggag ggcttcatca aagcgcagta
     1801 ccgcaagttc aacatgaagg gcgaggacct cgcgcccggc gacgactacg ccatgatgcg
     1861 cgagatgctg aaccggcgtt tcagccggct ggtcaaagaa agggacaagg accaaggcga
     1921 gggctgggcc gcgcccgatc tggttctgat cgacggcggc ccggggcagc tctccgcggc
     1981 gcaaggcgtc ctggacgatc tcggcctttc ggacatccgc atcgccgcgg tggccaaagg
     2041 cccggaccgc aatgccggcc gcgagcgcgt ctaccagaag gacaaggcgc ctttgctttt
     2101 ggaaccgcgc gaccctgtgc tttattgcat ccagacgctc cgggacgagg cccaccggtt
     2161 tgccatcggc gcccaccgcg cccggcgcaa gaaaggcatc accgcctcgc cactggatga
     2221 ggtgccgggc attggccccg cgcgcaaacg ggccttgctg aagtatttcg gttcggccaa
     2281 ggccgtcagc cgcgctggca ttaccgatct cgagggcgtt ggcggtatca gcgcacagat
     2341 ggcaaagacg atctatgatc acttccacga gcgcggctag ttgagcggaa cggactggat
     2401 atggcggaac aaggtttggc aggcggacgc ggaagcaatt ggaggcattt cggcagcctg
     2461 ccgaatatcc tgacctatgg ccgcattctt gcggtgccgg cactcgtcgc ctctttcttc
     2521 gtgacgggcg attggggccg gtggctggcc atgtggattt tcatcgccgc cggcgtcagc
     2581 gacttcctcg acggctatct cgcccgcgcc tggcagcaac aaagtgctct tgtccagatg
     2641 ctcgatccca tcgccgacaa gctgatcgtc gccgccgcgc ttctgatgct tgccgccgac
     2701 ggcacaatcg ccggctggtc gttgtgtgcc ggcgtgatca ttctttcccg cgaaattctc
     2761 gtgtcgggct tacgcgaatt tctcggtacg ttgaatgtcg gcgtgccggt caccaaactg
     2821 gccaagtgga aaaccttggt gcagatggtg gccattggct tcctgttggc aggctccgcc
     2881 ggcgacaaga tctggccgta cacgactctg tttggcctga cgcttctttg gattgccgcg
     2941 atcctcacga tttacaccgg ctatgactat ctgcgcgcca cgttccacca cgtggtccct
     3001 gaggacgagt aatgcgtctc ctgtatttcg cttgggtgaa agagaaagtc ggacacgccg
     3061 ccgaggacgt cgaagtgccg ggcgatgtcg ccaccgtcgc cgagctgatc gcctggctga
     3121 agacgcgcgg gcctgagttc gcgcatgcct tcgaacgcgc cgaagtaatc cgcgcggccg
     3181 tcgaccagac ccacgcgcgg cacgacgcga agatcggcga cgcgcgggag atcgccttct
     3241 tcccgcccgt caccggaggt tgaggtgatc cgcatccaga cccaagactt cgacgtcggc
     3301 gccgagatcg ccgcgctgcg gtccggccgc accgatgtcg gcgccgtcgt cagcttcacc
     3361 ggcaccgtcc gcgatcaagc cggccaagaa atgggcgacg tcgccgagat gacgctcgag
     3421 cactatcccg gcatgaccga gacggaactc gaacgcattg aagccgaagc aaacaaacgc
     3481 tggccactgc aagcttcgct catcgtgcat cgcgtcggca cgctgaaacc cggcaacaac
     3541 atcgtgctgg tcgtcaccgc ctccgcccat cgcgacgccg ccttcgacgc cgcgcgcttt
     3601 ctcatggact acctcaagac acacgcgccg ttctggaaac gcgagagcgg tccgtccggc
     3661 gacaaatggg tcgaggcgaa ggaaagcgac gaggccgcgg cgacgcgctg gtcgcgaaac
     3721 aacgcggccg agtagctagc gcacccggat gatgcgcgcg ttcggcggca gcactttctg
     3781 gtacggcccc cagacgcaga catcgcgcca ttcgcagaag ccgcgattgc agacccgctg
     3841 atatttgcag gagcggcatt tatagacggg ccggcagccc gaacagcgcg cgatctcctg
     3901 gcagtccctg aaggccttgg cgggcgcggc gatcggcgcg ggcgcggcat cgttcagccc
     3961 cggcagccaa ggggccccag cctggccgtc tccggccggc agcgccagca aagccattag
     4021 cccagccaat acgagcgtga tacgcacgag atcgcccccc taaatcgcgg tgcccccacc
     4081 gctggaccaa gagtgccctt ggagccctcc gggatcaagc atcgcgcgaa ggctcaccgg
     4141 cggataacga ccctgtccgg cgcgtgtcga tgctcccagc cagcccgctc cagcgccggc
     4201 gtgtcgtctt cttcttgcgg atagccaacg cataagagcc cgataaaggt ccaatccttg
     4261 ggcacgtcga ggatctccgc catgcaggcc ggatcgagga tcgacaccca gcccagcccc
     4321 aagccttcca cccgcgcggc aagccacagc gtatggatcg ccatcaccac ggagtaatcg
     4381 atggtctccg tcatggtgag acggccaagg cccgagcctt gggtcgtgtc ccggtcggcg
     4441 aatacggcga tttgtaccgg cgcttcgtcg agccccgcca gcttgagccg cgcatagagc
     4501 gccgcccggt cgggctcctg catggccagc gcgtctttgt tgcaagcctc gaaattggcg
     4561 cggatggcgg cgcggcggcc ggcggcctcc accagcacga accgccaggg ctcgctgagt
     4621 cccacggacg gggccagaca cgccaagccg atcagccggt cgagcatgcc ttcgggaagt
     4681 gcatcgcgct tgaagcggcg cacgtcccgc cgccagcgca gcaggtcgta aagccgcgcg
     4741 cggaacgcgg cgtcgaacgc cggtgccgcc ttgggcggct cttcttccgt tggcgtgcgc
     4801 tgcacgactt gtgggctgag cgaactcatg gcgggcgaca ttagcacggc tcgcctcgcg
     4861 ccaagcccgc tcttatccgc gcctcccgat acaggaatac agtcctagtg cggttcgaac
     4921 ggacgtcttg tgtaggaaaa tcgtggaaga acggctcgat ggtgctactg ccgaagaaga
     4981 gtgactgcag ccaaacagtg cctaccccgc cgcggcgacc ttggcctgcg cgggccgcac
     5041 cagcgcggcg tagtcgttca tcagcgtgcg cgtaatgtcg ccgacttcga acgtgtagcc
     5101 gccgatcgaa ccgaccggca tgacctctgc ggcggtgccg gtgatgaagc actcggtgaa
     5161 atcggcaagc tcttccggca tgatgcccgt ctcgatcact ttccagccgc gctgcttggc
     5221 gagaccgatg accgtgcgcc gcgtgatgcc gtcgaggaaa cagtcgggcg tcggcgtata
     5281 gatcaagccg tcctt
//
LOCUS       DBNHVU010000117        21075 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_712853, whole genome shotgun sequence.
ACCESSION   DBNHVU010000117 DBNHVU010000000
VERSION     DBNHVU010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 21075)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21075)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21075
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_712853"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..464)
                     /locus_tag="AC16Y7_04570"
     CDS             complement(51..464)
                     /locus_tag="AC16Y7_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-translocating transhydrogenase family
                     protein"
                     /protein_id="HIR5708688.1"
                     /translation="MTRRTLNTVLALFAVAMLAELAWMGQAFAAGAAEEGHDFISQFS
                     IFLLAVFIGYYVVWSVTPALHTPLMSVTNAISSVIVVGALLAVAVEASEAFSFSGHWL
                     AKILGLVALILASVNIFGGFLVTQRMLAMYRRKDR"
     gene            complement(461..1732)
                     /locus_tag="AC16Y7_04575"
     CDS             complement(461..1732)
                     /locus_tag="AC16Y7_04575"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HIR5708689.1"
                     /translation="MIAIGVPGEGKDEPRIGLTPETVKKLVKAGAKVTVRSGAGLRSH
                     FSDADYKEAGAAIAKSDAAAVAKADVVFTVRRPSLPLAKAMKKGAAVMGMLDPFSDAR
                     GLEALAKTGVSLFSMELMPRITRAQSMDVLSSQSNLAGYKAVADAAAAFERALPMMMT
                     AAGTVPAAKVFVMGAGVAGLQAIATARRLGAIVTATDVRPAAKEQVESLGAKFIAVED
                     EEFKQAETSGGYAKEMSDAYKKKQAKLVADHVKAQDIVITTALIPGRPAPKLISMAMI
                     EGMKPGSIIVDLAAERGGNTDVTKTGKIAEHKGVRIFGKLNLPGELPVNASSLYARNL
                     QAFVDPFIDKEKKVLAIDWEDELAIGTAIARDGAIVNEMVAGNLGARGGSAAKSAAKG
                     RAKTFVRTTSARKTSARKTPAKRRPRGGKKT"
     gene            complement(1822..2040)
                     /locus_tag="AC16Y7_04580"
     CDS             complement(1822..2040)
                     /locus_tag="AC16Y7_04580"
                     /inference="COORDINATES: protein motif:HMM:NF019452.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HIR5708690.1"
                     /translation="MPWILLSGVKRAKPRPKDGTPGGSRMHIDPKEGHPDMDYAEHMA
                     TYRMFCKFTLWSVIIIAVIMALMAYFLT"
     gene            complement(2077..2994)
                     /locus_tag="AC16Y7_04585"
     CDS             complement(2077..2994)
                     /locus_tag="AC16Y7_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5708691.1"
                     /translation="MPVTELVRVVVTVADPAAMAGFYHEALGLDVGPEQALGDPAWSA
                     LLGLPEGTTIRVASVRFGSETLALASFDPPGAPYPATRASNDQWFQHVALVAGDIEAV
                     WKRLERAKPEVITKGEPVLLPPNTGSVTAFKFRDPEGHPLELISFPKSVGDPRWQGGA
                     PGIRGYDHTAISVTDLDRSLPFYTDLLGFHVGGRSLNAGPEQDRLDGLAGIQVDVVAL
                     QPVSAPTPHVELLHYRTPPGRKSAAEIKANDVASVRQVHRVDDLAGLVARLQAAQVPF
                     VLPGIVAFQDGGQAAAVRDPDGHMIVLLA"
     gene            complement(3006..3491)
                     /locus_tag="AC16Y7_04590"
     CDS             complement(3006..3491)
                     /locus_tag="AC16Y7_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5708692.1"
                     /translation="MAEPIGNNITQEGNGPPELSYWHVWADENGVTHQTRCALSDFKE
                     ESVGGKAAPEWIETLLTSKANVVFFVQPVGWVGDWHPNPKPQWIVTLSGRWFVETMDR
                     TRVEMGPGEVSFGGDQTTKADAQGRFGHRSGTVGDELLSLMAIQLDGEEWLGLKPCSF
                     K"
     gene            complement(3494..3970)
                     /locus_tag="AC16Y7_04595"
     CDS             complement(3494..3970)
                     /locus_tag="AC16Y7_04595"
                     /inference="COORDINATES: protein motif:HMM:NF019301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR5708693.1"
                     /translation="MDWLINDALPVLARIFIVWIFPFSFLDKIINHEAAVKQVSSGWL
                     PGGAALLYPAMAVELLTPPMIVFGFYDGIAAFILAGYCAITALLFHNFWSYPRFWSSD
                     SEGYSHVWDFFKNFGLVGGLIFVMLASGFFQSAETEIEEVTVLGNHAQAQASRNRE"
     gene            complement(4038..5426)
                     /locus_tag="AC16Y7_04600"
     CDS             complement(4038..5426)
                     /locus_tag="AC16Y7_04600"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HIR5708694.1"
                     /translation="MTDKPSDDEKNRFTARAKRYGRVGANVGGVAARIAGRRLFGLDP
                     ERDKDAAALATALGGLKGPIMKVAQLLATIPEALPAEYAAELGQLQSQAPAMGWSFVR
                     RRMAAELGPDWQSEFARFEHEAAASASLGQVHRAAAHDGCRLAVKLQYPDMQSAVEAD
                     LSQLGILFSLHRRMQPAIETSQMLEEISARLREELDYELEAKHTRLYGLIFEDDPVIR
                     VPDVLPELSTKRLLTMTWLDGRRLLDYTDRPLEERNAIARAMFRAWWFPFSHYGVIHG
                     DPHLGNYTVFEDAGGKAAGINLLDYGCMRTFAPKFIQGVVDLYHGLLNDDRALTVHAY
                     ETWGFGGLSNELIDILNIWANFIYGPMLEDRVRTIADTGTPGSYGRKEAFKVHQGLQE
                     KGPVTVPREFVFMDRAAIGLGGVFLHLNAELNYYRLFNDAIAGADLADVTWRQRETFA
                     KAGVPLPEGSSS"
     gene            5534..6469
                     /locus_tag="AC16Y7_04605"
     CDS             5534..6469
                     /locus_tag="AC16Y7_04605"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR5708695.1"
                     /translation="MTKIFHAIAALGLAGGAALLAIGVLAHWYPALDIVNNGLPFLAV
                     GAGALLALAFALRSRRLIGATAILLAATLIVLLLGLQGRAPQAPRDAERFLRVATFNL
                     WGRNDDHLNRVAEFVAETDADAVVLEEVRRHHGAFVDSLGELYPFRVGGNGLFILSKH
                     PILADGQTDRPDQPHWMSRIIRWVRLDVNGKAVTLAGVHLARPFYPELQQADIEALAR
                     FVKAQDGPLILAGDFNMTPWTVKMRTFTEVTGLGRFNTFNPTWPMRWRSVPLLPLLPI
                     DNVFASAHFANISTRLGPRLDSDHRAVIADLALIE"
     gene            6496..6939
                     /locus_tag="AC16Y7_04610"
     CDS             6496..6939
                     /locus_tag="AC16Y7_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HIR5708696.1"
                     /translation="MALRTVLAFCFALLFTAPALGTPSIEVWKSPTCGCCGKWVEHLE
                     ANGFAVTAKNTSPGMLDRIKRQAGIGEKLASCHTGLIDGYSVEGHVPAADIKRLLEER
                     PDAIGLTVPKMPIGSPGMEHPSGETEPYDVLLVKKDGSTEVFARH"
     gene            complement(6958..8823)
                     /locus_tag="AC16Y7_04615"
     CDS             complement(6958..8823)
                     /locus_tag="AC16Y7_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006472903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR5708697.1"
                     /translation="MLRNFPLRHPLPVFAAAETAKSQKTLGELPEWDLGALYDAPESP
                     RLKNDLEQAERAADDMHTRYAGKLAALLDGGNGGAALAEAVRSYEALNDLLGRIVSYA
                     SLRYSANTADPARQKFYGDIQERITAISSKLLFFPLELNRLDDAALETAMTGAELGHF
                     RPWLEDLRKEKPYQLEDKLEQLFHEKSVTARGAWDRLFNETMTALRFDVKGEELSLEP
                     TLNLLLDADEKKREHAAKALGKVFEENVRVFTLITNTLAKDKEISDRWRGFEDVADAR
                     HLSNRVEREVVDALVAAVRDAYPRLSHRYYAMKAKWLGKKKLMYWDRNAPLPEEPKQV
                     IGWDDARETVLTAYGDFSPRMSDIAGRFFTDGWIDAPVREGKQQGAFSHPTVPSAHPF
                     ILLNYQGKPRDVMTLAHELGHGVHQVLANKQGPLMAPTPLTLAETASVFGEMLTFRKL
                     LDNAPSKRERKALLAAKVEDMLNTVVRQIAFYSFERKVHAERREGELTSERLGELWMD
                     VQRESLGPAIELKPEYETFWSYIPHFIHAPFYVYAYAFGDCLVNSLYAVYEKADAGFA
                     ERYFDMLAAGGTKHHTELLKPFGLDASEPHFWSMGLKVIEGLIDELEAMDEKGEA"
     gene            9087..10592
                     /locus_tag="AC16Y7_04620"
     CDS             9087..10592
                     /locus_tag="AC16Y7_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR5708698.1"
                     /translation="MPKRVLVVDDDPAQRRILEETITRFGYHVETAEGGDAALAALKA
                     DTAGDICLLLLDLVMPETDGMAVLSAMRSLPRKAPTIVQTAHGSIDAAISAMRAGAVD
                     FVVKPASPERLEVSIKNALKIEALAGEITRMKATANGDLSFANLIGSDTMKRVIELGN
                     RAAKSSIPVLIEGESGVGKELIARAIHGESARQAKPLVTLNCGAIPENLVESILFGHE
                     KGSFTGANEKRIGKFVEADGGTLFLDEIGELPLDAQVKLLRALQEGEIDPVGSKQSVK
                     VDFRLISATNQNLITMVQEGRFREDLYYRLNVFPIWIPPLRERLEDVPELVRHFTARF
                     AAEEGKHIDGIDAEAAAMLERYTWPGNIRQLENAVFRAVVLADTPMLTVNEFPQIAAH
                     VEGYAVTVPPAPAPKDPAPHIEGPVMLGQSATAPATIHVPSGNGKDSVGIPALSDSGD
                     IRSLEAVEADMIRLAFGRYRGRMTEIAKRLGIGRSTLYRKMREIGIEARPN"
     gene            10645..12558
                     /locus_tag="AC16Y7_04625"
     CDS             10645..12558
                     /locus_tag="AC16Y7_04625"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR5708699.1"
                     /translation="MTTRPVHGIALTLLLAASPAVAVERDASDADTVAIEMPEPKSQA
                     RLSAPGSVSETPEASRTSETNEVPKTPAVAETRPSTTEPIRRAVQSRLSQSFTATSTQ
                     KKTEKGALAEYYATPNQKLLWVDETGLTARGKAVIAEIERADDYGLRASDYTLPNMAG
                     FETSASDATEKLADAEIAISHAVLDYAHDARGGRIKPSRISRNLDPTLNLPKPMEVIE
                     SLSFRSDPAAYLRSFQPQHPQFELLRKKLVELRGGGQQAAKPEKPKVKIPAGQTLKLG
                     IADPQVALLRTRLDVPAGDNPEMYDEALAEAVKRFQAEHNTASDGAVGPGTRRLLNNP
                     SLRDMGSPAQIKKILLNMERWRWLPQDQGSFFVTVNVPEFTLRVVKDDEPIHTSRVVV
                     GKLKTQTPIFSNEMKTVVFGPYWNVPTSIKVGEIRPYVRPTGGWFGGGWNTSVFQRHD
                     LRVKFGGREVDPASLDWNRVDIRKLHLYQPPGPRNVLGRVKFVFPNKHDVYMHDTPQK
                     HLFAKRVRAESHGCMRVQNPDQLAAVILKHDRSWDDARTLSAFRSGYNQHVGLNRNVP
                     VYITYFTMRVNEDGSITTFGDLYGHDGRMQTALLDGRTAYAEADDGIITQSIPRRRRS
                     APSRDRWERLLDF"
     gene            12852..14063
                     /locus_tag="AC16Y7_04630"
     CDS             12852..14063
                     /locus_tag="AC16Y7_04630"
                     /inference="COORDINATES: protein motif:HMM:NF017736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan L,D-endopeptidase MepK"
                     /protein_id="HIR5708700.1"
                     /translation="MRIRGIHARRAMIGAGVLLAALGAAVAPNAFASDTRTLSLYEIH
                     TKERLTVTYKRDGEFDDEALEKLNYFMRDWRTDEPTKMDRELIDLIWTLHTQLGSQEP
                     IHLISGYRSAKTNAKLRRRGGGQARKSQHIQGRAADIHFPDVPVKKLRASALIQEVGG
                     VGYYPTSGIPFVHVDTGRVRMWPRMPRLELAALFPDGKTGYVPRDGRRITKKDHERAL
                     AKGMVNKTLLAAANPATPPPTPKPQPIMASFTPPMALAPADKPATPAPQLASANAGGG
                     VLPWLQSRLSPEPKRPAVPTYEKTQVVGPPKVDDDHPDELSYLPFETASLMTDTSVAR
                     NPEIAPLSHPEQKDLGYLFEDMDRPTAFKLRRTSGYLGLASVQTFSGRAVKSLYAAID
                     AQTGPTRLAQR"
     gene            complement(14067..14357)
                     /locus_tag="AC16Y7_04635"
     CDS             complement(14067..14357)
                     /locus_tag="AC16Y7_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006932199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HIR5708701.1"
                     /translation="MSLQSSAKDQLRAFVSRIERLEEEKAALAADLREVYAEAKGNGF
                     DAKALRKVISLRKLDDNKRREEDAVLTTYLHALGMDGEAGSDAGAAATEAAA"
     gene            complement(14535..14789)
                     /locus_tag="AC16Y7_04640"
     CDS             complement(14535..14789)
                     /locus_tag="AC16Y7_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HIR5708702.1"
                     /translation="MDETTKTEIEAAAFRRLVEHLRERTDVENIDLMELAGFCRNCLS
                     TWVLEAADERGVPLAKDEARELIYGMPYEDWKAKFQTERS"
     gene            complement(14782..15621)
                     /locus_tag="AC16Y7_04645"
     CDS             complement(14782..15621)
                     /locus_tag="AC16Y7_04645"
                     /EC_number="3.5.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HIR5708703.1"
                     /translation="MRLDICVQDDLGEKPYRTIEGDARLGLLILCDHAQNRIPDGYHD
                     LGLRHEDLHRHMAVDLGAEGVAEMLATMVGAPAVLSRFSRLLIDPNRGLDDPTLIMQL
                     SDGLMVPGNVGLSAEQMEARLDQFYRPYHAAVERAVDAGIAAGKPPVIVSIHSFTQAW
                     KGKPRPWAAAVLWDKDPRMPVPLIEALRTIPDVEIGDNEPYSGQLKGDTLYRHATSRG
                     LAHALIEVRQDLILSEEGQGIWAAHLARTIADVLNAAPGLHTIEHHGSNTGLCKAKDD
                     ANG"
     gene            15850..16335
                     /locus_tag="AC16Y7_04650"
     CDS             15850..16335
                     /locus_tag="AC16Y7_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HIR5708704.1"
                     /translation="MLANKNIRNPARTFTTAWSRWGIAASVALGLTTGFDVAPAHADL
                     KLCNTTASRVGVSIGYKDTEGWASEGWWNIASHTCETLLKGVLIGRFYYIHAIDYDRG
                     GEWAGALFMCTDDKSFTIRDTKDCEKRGHKRTGFFEVDTGEERDWTVRLTDPDGEAKP
                     Q"
     gene            16332..17762
                     /gene="pyk"
                     /locus_tag="AC16Y7_04655"
     CDS             16332..17762
                     /gene="pyk"
                     /locus_tag="AC16Y7_04655"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR5708705.1"
                     /translation="MRRNRRVKIVATLGPASTNEETMRRLFEAGADVFRVNMSHAQHD
                     ALREIHGIVRKLESEYSRPIGILVDLQGPKLRIGKIDSGSIRLTQGDILSFGRGEDVG
                     NLGRIQLPHPEIFESVEKGHTLLLDDGKLKLRVVEASDVRIDAEVLSSGLLGSRKGIS
                     LPDTVLPMGALTDKDRADIECAMSLGVAWMALSFVQRPEDIAEARKLSRGRTSIMAKI
                     EKPTAINHLKEIVDLADGIMVARGDLGVEMPVEKVPGLQKQISRAARNAGKPVVVATQ
                     MLESMIYAPVPTRAEVSDVATAVFEGADAVMLSAESAVGKYPVDAVATMNRIAVEVEH
                     DPLHAAIIHAQRIDPTATDADAISAAARTVAETLNLAAIVCYTASGSTSLRAARERPQ
                     QPILALTPIPATGRKLAIVWGLHCVLTDDPRNLDDMVAKACRIAYQEGFALPGEQVII
                     TAGVPLGKSGATNLLRIAEVDPGTDE"
     gene            complement(17805..18596)
                     /locus_tag="AC16Y7_04660"
     CDS             complement(17805..18596)
                     /locus_tag="AC16Y7_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708706.1"
                     /translation="MSTVRRFAGLFVLALSVSFAGTAFASPPLVLAQFFEHFDEETDE
                     ARPPADDPALEGLDLPGLEGLEPGAGQSADGSQDELELGLDLDPSLDPSLGGAPFDPA
                     QRPKLLAELYEKLGAARSATAAEPIMEAIEDLWMVSGSDTVDLLMSRAARFAKEAEID
                     LSLAVLDAVVDIAPDEAEAWYLRAKVNVLKGKPERALSDLRRALNLDAKHYRAIADLG
                     LLLEQLGAKKEALEAYRRALAVNPFLEDAQAGVDALKREVEGQDI"
     gene            complement(18686..18811)
                     /gene="ykgO"
                     /locus_tag="AC16Y7_04665"
     CDS             complement(18686..18811)
                     /gene="ykgO"
                     /locus_tag="AC16Y7_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012709334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HIR5708707.1"
                     /translation="MKIKNSLRALRKRHRNNRVVRRRGRLYVINKTNRRFKARQG"
     gene            complement(18993..19253)
                     /locus_tag="AC16Y7_04670"
     CDS             complement(18993..19253)
                     /locus_tag="AC16Y7_04670"
                     /inference="COORDINATES: protein motif:HMM:NF025051.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR5708708.1"
                     /translation="MKILAAAFALTFAMATSAFADDRALTEQEIEGVQKAIQAMGCTV
                     ADSEIEAEKSGYEAEDVICEDRKQYEVYLDKDFKVIKKVEED"
     gene            19438..19719
                     /locus_tag="AC16Y7_04675"
     CDS             19438..19719
                     /locus_tag="AC16Y7_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708709.1"
                     /translation="MHTDTYLFVVCARRNASSIFGAASNPVMRNGKLLCFKTEAGARA
                     ERDRLTARLGEAHVRYSVRPVHVQLKPPHEAAKVEAAPPSRAMPMPEAA"
     gene            19797..20219
                     /locus_tag="AC16Y7_04680"
     CDS             19797..20219
                     /locus_tag="AC16Y7_04680"
                     /inference="COORDINATES: protein motif:HMM:NF015669.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HIR5708710.1"
                     /translation="MQRYRLMAQNALCAIATIFFTAVFAAGVAMSADISLAGSEWGFA
                     NETGKPARYIQFAEDQASGNLGCNRFTGGYSFKDGELNFRPLASTRMACAPEAMKREQ
                     EFAALLSKVRFAEATHLKLVLKDADGEVLAELVRRDPD"
     gene            complement(20372..>21075)
                     /locus_tag="AC16Y7_04685"
     CDS             complement(20372..>21075)
                     /locus_tag="AC16Y7_04685"
                     /inference="COORDINATES: protein motif:HMM:NF013132.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR5708711.1"
                     /translation="SLGEAKTDVAGEMLREMGPMSVPERRVAIIFAVVAAAWIFRPLV
                     HDWLGAGLTDTGIAIAGAIALFVVPANWRERTFLLGRMAVRKVPWEVLILFGGGLSLA
                     KAIEVSQLATWMGNGLSFLHSMPLVVLVFGVALLVALLTEWMSNAATAAAFLPIAGSL
                     ALGSDIAPLLIVFPIALAASSAYMLPVATPPNTLVFGTGYVTIPQMIRAGALLTFLGA
                     AIIAAGVTLLAPVFW"
BASE COUNT         4066 a         6906 c         6314 g         3789 t
ORIGIN      
        1 acaggacgcc agcggcgaga taaagcagcg cgactagatc acccgacatc ttagcgatcc
       61 ttacgccggt acatggcgag catgcgctgc gtcaccagga agccgccaaa gatattgacg
      121 ctagccagga tcagcgcgac caggcctaag atcttcgcca gccaatgccc cgaaaaggag
      181 aaagcctcag aggcctcgac ggcgacagcg agcagcgcgc caaccacgat cacggacgag
      241 atggcgttgg tcaccgacat gagcggtgta tgcagcgccg gcgtcacgct ccacaccacg
      301 tagtacccga taaacacagc aagcaggaaa atcgaaaact gcgagataaa gtcgtggccc
      361 tcctcagccg ctcccgcggc gaaggcctgc cccatccacg ccaactcggc cagcattgcc
      421 acggcgaaga gagcaaggac cgtattcagg gtccgtctcg tcatgtcttc ttacctcccc
      481 tcggcctgcg tttcgcgggc gtctttcgcg cggacgtctt tctggccgat gtcgttctca
      541 caaatgtctt ggcccggcct ttggctgcgg atttggccgc gcttcccccg cgcgcaccca
      601 agttcccggc gaccatttcg ttgacgatgg cgccgtcgcg ggcaatggcc gtgcctatgg
      661 cgagctcatc ctcccaatcg atggccagga ccttcttttc tttgtcgatg aacggatcga
      721 cgaaggcctg aagattgcgg gcatagagac tcgacgcatt gaccggcagc tcgcccggaa
      781 gattgagttt gccgaaaatg cgcacgccct tgtgctcggc gattttgccc gtcttcgtca
      841 catcggtgtt gccgccacgc tcggccgcca agtcgacgat gatggagccg ggcttcatgc
      901 cttcgatcat cgccatggag atcagcttgg gcgccgggcg tccggggatc aatgccgtgg
      961 tgatgacaat gtcctgagcc ttgacgtgat cggcgacgag tttggcttgc ttcttcttat
     1021 aggcgtcgga catctccttg gcgtagccgc cggatgtctc cgcctgcttg aattcctcgt
     1081 cctcgacggc gatgaacttc gcgccgagcg actcgacctg ttccttcgcc gccggacgca
     1141 catccgtcgc cgtgacgatg gcgccgagac gccgcgccgt ggcgatcgct tgcaggccgg
     1201 cgacaccggc acccatgacg aagaccttgg ccgcgggcac cgtgcccgcc gcggtcatca
     1261 tcatcggcaa tgcgcgttcg aacgcggcgg ccgcgtcagc cacggccttg tagccggcga
     1321 ggttcgattg cgacgacagc acgtccatgc tctgcgcgcg cgtgatgcgc ggcatcagct
     1381 ccatggaaaa cagcgagaca ccggtcttcg ccagtgcctc aagcccgcgg gcgtcggaga
     1441 aaggatcgag catgcccatg accgccgcac ccttcttcat ggccttggcg agcggaagcg
     1501 atgggcgccg tacggtgaac accacgtcgg ctttggcaac ggcggcggcg tcgctcttgg
     1561 cgatcgcggc gcctgcctcc ttgtagtccg cgtctgagaa atgagacctg agcccagcgc
     1621 cggaccgaac agtgaccttg gccccggcct tcacgagctt cttgacggtc tcgggcgtga
     1681 gcccaatccg cggctcgtct ttgccttcac caggtacgcc aatcgcaatc atcgtggctc
     1741 cctcagcgga cgaacgaggc agcgcagccg accgtccgcg tagaatgcat cttgaagttt
     1801 cgagatgagc taaagcgagg tctacgtcag gaagtaggcc attagagcca tgatcaccgc
     1861 gatgatgatc actgaccaca aggtgaactt acaaaacatt ctgtaagtcg ccatgtgctc
     1921 ggcgtagtcc atgtcgggat gtccctcttt gggatcaata tgcattcgac tgcccccagg
     1981 tgttccgtct ttagggcgcg gcttggcgcg cttgacgcca gatagcaaaa tccagggcat
     2041 cccgcaatga ttgctgccct ttcctcgccc tcaactttag gccagcagca cgatcatgtg
     2101 gccgtcgggg tcccgcaccg cagcggcctg cccaccgtcc tgaaaagcca cgattcccgg
     2161 caagacgaag ggcacctggg ccgcctgaag ccgggccaca agccctgcga gatcgtccac
     2221 ccgatggacc tggcggacgg aggcaacatc attggccttg atttcggcag ccgattttcg
     2281 gcccggcggc gtccggtagt gcaacagctc cacatggggc gtgggcgcgg ataccggctg
     2341 aagcgccacc acatcgacct ggatcccggc aagcccatcg agccggtcct gctcaggccc
     2401 tgcgttcagg gagcgcccgc ccacatggaa gcccagcagg tcggtataga agggaaggct
     2461 ccgatccagg tccgtcacgg agatcgccgt gtggtcgtaa ccgcgaatac cgggcgcacc
     2521 gccctgccag cgtggatcgc cgacgctctt cggaaaggag atcagctcca gcggatggcc
     2581 ttccggatcg cgaaacttga aggccgtgac gctgccggtg ttgggcggca ggagcaccgg
     2641 ctcgcctttg gtgatgactt caggctttgc ccgttcgaga cgtttccaga cggcttcgat
     2701 gtcgccggcg acgagcgcca cgtgctgaaa ccactgatcg ttcgaggcgc gtgtcgccgg
     2761 atacggcgcg cccggaggat cgaacgacgc gagcgcgagc gtttcagagc caaaccgaac
     2821 ggaggcgacg cgtatggttg tcccctcagg aagaccgagt aacgcgctcc aggccggatc
     2881 gcccagcgcc tgctcggggc ccacgtcgag ccccagcgcc tcgtggtaga agcccgccat
     2941 cgcggccgga tcggcgacgg ttacgacgac ccggacgagc tcagtgaccg gcattggcgt
     3001 tcacctcact tgaaactgca tggcttcagt ccgagccact cctcgccgtc gagttggatc
     3061 gccatcaggg acagaagctc atcgccaacg gtccccgagc ggtgcccgaa gcggccttgc
     3121 gcgtccgcct tggtggtttg gtcgccgccg aaggagacct cccccggccc catttcgacc
     3181 cgcgtgcggt ccatcgtctc gacaaaccat cggcccgaca gtgtgacgat ccactggggt
     3241 ttgggattgg ggtgccagtc cccgacccag ccgaccggct gtacgaaaaa gacgacattg
     3301 gctttggagg tcagcagcgt ctcgatccat tcaggcgccg ctttgccgcc tacgctttcc
     3361 tctttgaaat ctgaaagcgc gcaccgcgtc tggtgcgtga caccgttctc gtccgcccag
     3421 acatgccaat aggagagctc cggcggaccg ttgccctctt gggtgatgtt gttgccgatc
     3481 ggctccgcca tgctcactcc cgattgcggc tcgcctgcgc ttgcgcatgg ttgccgagaa
     3541 ccgtcacctc ctcgatctcc gtttccgcgc tctggaaaaa gccgcttgcg agcatcacga
     3601 agatcagtcc gccgacgagc ccgaaattct tgaagaaatc ccagacatgc gaataaccct
     3661 cgctgtccga cgaccagaag cgcgggtagg accagaagtt atggaacagg agcgcggtga
     3721 tcgcgcagta accggcaagg atgaatgcgg cgatgccgtc atagaagccg aacacgatca
     3781 tcggcggcgt caacaactcg accgccatgg ccggatagag cagcgccgcg ccgccgggaa
     3841 gccaacctga ggacacctgc tttaccgctg cctcgtgatt gatgatcttg tcgaggaagc
     3901 tgaacggaaa aatccagacg atgaaaatgc gcgccagcac gggcagcgcg tcgttgatca
     3961 gccaatccat cgtcagccct cctctcgata gattcgcaag ggcgaatcct ctgcgaagtg
     4021 agggccgatc acaaccttta agaggacgat ccttccggca gtggcacgcc ggccttggcg
     4081 aaggtctcgc gctggcgcca cgttacgtcg gcgagatcgg cgccggcgat ggcgtcgttg
     4141 aacaggcggt aatagttgag ctccgcattg aggtgcagga acaccccgcc gagcccaatt
     4201 gcagcgcggt ccatgaatac gaactcccgc ggcaccgtca ccgggccctt ctcctgcagg
     4261 ccctggtgca ccttgaaggc ctctttgcgg ccatagctgc caggcgtccc cgtatcagcg
     4321 atggtgcgga cccggtcctc gagcatcggg ccatagatga agttcgccca gatgttcaaa
     4381 atgtcgatga gctcgtttga gaggccgcca aagccccagg tctcgtaggc atgcacggtg
     4441 agcgcacggt cgtcgttgag aaggccgtga tagagatcta ccacgccttg aatgaatttc
     4501 ggcgcgaagg tccgcatgca gccatagtcg agcagattga tgccggcggc cttgcctcct
     4561 gcatcctcga acaccgtgta gttgccgaga tgcggatcgc cgtggatcac cccgtagtgg
     4621 ctgaagggga accaccaggc gcggaacatg gcgcgcgcga tggcgttgcg ctcctccaat
     4681 ggccggtccg tatagtcgag aagcctccgc ccatcgagcc aagtcatggt gaggaggcgc
     4741 ttggtcgaca gctccggcaa cacgtccggc acacggatca cgggatcgtc ctcgaagatg
     4801 agaccgtaaa gacgggtgtg cttggcctcc agctcgtagt cgagttcttc acggagccgc
     4861 gcggaaattt cctccagcat ctgcgaggtc tcgatcgcgg gctgcatgcg ccgatgcagc
     4921 gaaaacagaa tgccgagttg ggagaggtcc gcctccaccg ccgactgcat gtccggatat
     4981 tgcagcttca ccgcaaggcg gcatccgtca tgcgccgccg cccggtgcac ctgcccgagc
     5041 gaggcggacg ctgccgcctc gtgctcgaac ctggcgaatt ctgattgcca atccggtcca
     5101 agctccgccg ccatgcgccg ccgcacgaag gaccagccca ttgccggcgc ttggctctgc
     5161 aattggccca gctcggcggc gtattcggcc ggcaatgcct cggggatggt ggcgagaagc
     5221 tgcgccacct tcataatcgg cccttttagg ccgcccaacg cagtggcgag agccgccgcg
     5281 tccttatccc gctcgggatc gagcccaaag aggcgccgcc cggcgatacg ggccgccacg
     5341 ccgccgacat tggcgcccac ccggccatag cgtttggcgc gtgcggtgaa ccggttcttc
     5401 tcgtcgtcgg atggcttgtc ggtcatcggc aacaaaaaag ctttcacaca tgggtaaacc
     5461 ttccctcata tagggacgct tcccggccgc cgcccgcttc tttcgcatct gccgtcaagg
     5521 acttgccaat tcgatgacga aaattttcca cgcgattgcc gctctcggtc tggctggcgg
     5581 cgcggcactt ctcgcaatcg gcgtgctggc ccattggtat ccggcgctcg acatcgtcaa
     5641 caacggcctg cccttccttg ccgttggcgc aggtgccctg ctggcgctcg cctttgcctt
     5701 gcgctcgcgg cggctgatcg gggccaccgc catcctgctc gctgccacgt tgattgtgct
     5761 gctcttgggc cttcagggac gcgccccgca agcgccgaga gacgcggagc ggttcttgcg
     5821 ggtggcgact ttcaatctgt ggggccgtaa cgacgaccac ctgaaccgcg ttgccgaatt
     5881 tgtggccgag acggatgcgg acgccgtggt gcttgaggag gttcgccgtc accatggcgc
     5941 cttcgtcgac agccttggcg agctctaccc gttccgtgtc ggtggcaacg gcctctttat
     6001 tctgtcgaag caccccatac tcgccgacgg ccaaacggac cggcccgacc agccccattg
     6061 gatgtcgcgc attatccgct gggtccggct cgacgtgaac ggcaaggccg tcacactggc
     6121 cggcgtgcat ctggcgcggc ccttctaccc ggaacttcag caagccgaca tcgaagctct
     6181 cgcgcgcttt gtgaaagcgc aagatggccc gttgatcctc gccggcgact tcaacatgac
     6241 gccgtggacg gtgaagatgc gaaccttcac ggaagtcacg gggcttggcc gcttcaacac
     6301 cttcaatccg acatggccca tgcgctggcg aagcgtgccg cttctgccgc ttttgcccat
     6361 cgacaatgtg tttgcctcgg cgcacttcgc caacatctcg acgcgcttgg gcccgcgcct
     6421 cgattccgat caccgtgctg tgatcgccga cctcgcgctg atcgaataga atcgggcgac
     6481 aggaggagta cccccatggc tttgcgaact gttcttgcgt tttgcttcgc gctcctattc
     6541 accgcacctg cacttgggac gccgtccatc gaggtctgga agtccccgac ctgcggctgc
     6601 tgcggtaaat gggtggagca tctggaggcg aacggctttg ccgtgacggc gaagaacacc
     6661 tcgcccggca tgctcgaccg catcaagcgt caggccggca tcggagaaaa gctcgcctcg
     6721 tgccacacag gactgatcga cggttactcc gtggaaggcc atgtccccgc cgctgacatc
     6781 aagcggcttc tggaagagcg gcccgacgcc attggtctca ctgtgccgaa gatgccgatc
     6841 ggctcgccgg gcatggaaca ccccagcggc gagaccgaac cgtatgacgt gttgctggtg
     6901 aagaaggacg gcagcaccga ggttttcgcc cggcactgac gcgcgcgcgg cgtctcctta
     6961 agcctcgccc ttctcgtcca tggcctcgag ttcgtcgatc aggccttcga tcaccttgag
     7021 tcccatcgac cagaaatgcg gctccgacgc atccagccca aacggcttga gcaattccgt
     7081 gtggtgtttg gtgccaccgg ccgcgagcat gtcgaaatag cgctcggcga agcccgcgtc
     7141 cgccttctcg tagacggcgt agagcgagtt caccagacaa tcgccgaagg cataggcgta
     7201 gacatagaac ggcgcatgaa tgaagtgcgg gatgtaggac cagaaggtct catattccgg
     7261 cttcagctcg atcgcagggc caaggctctc gcgctgcacg tccatccaga gctcgccgag
     7321 acgctcggac gtcagttcgc cctcacgccg ctccgcatgc accttgcgct cgaaactgta
     7381 gaacgcgatc tgccggacga cggtgttgag catgtcctcg accttggcgg cgagcagcgc
     7441 cttgcgctcc cgcttgcttg gcgcattatc gagcagcttg cggaaggtca gcatctcgcc
     7501 gaacacgctc gccgtctcgg caagcgtcaa cggcgtcggc gccatgagcg ggccctgctt
     7561 attggcaagc acttggtgca cgccatgccc cagctcgtgc gcgagtgtca tcacgtcgcg
     7621 cggcttgccc tggtagttca gaagaataaa gggatgcgcg ctcggcactg tggggtgtga
     7681 gaaggcacct tgctgtttgc cttcgcgcac cggcgcatcg atccagccgt cggtgaagaa
     7741 gcggcccgcg atgtcggaca tgcgcggcga gaaatcgcca tacgccgtca gcacggtctc
     7801 acgtgcatcg tcccagccga tcacctgctt aggttcttcg ggtagaggcg cgttgcggtc
     7861 ccagtacatg agctttttct tgccaagcca cttggccttc atcgcgtagt agcggtgcga
     7921 gaggcgcgga taagcgtcgc gcacggcggc gaccagcgcg tccaccacct cgcgttcgac
     7981 ccggttggac agatggcgcg cgtcggcaac gtcctcgaag ccgcgccacc ggtcggaaat
     8041 ttccttgtcc ttggcgagcg tgttggtgat cagcgtgaac acccgcacgt tctcctcgaa
     8101 caccttgccc agggccttag ccgcgtgttc gcgcttcttc tcgtccgcat cgagcagcag
     8161 attgagcgtc ggctctaggc tgagctcctc gcctttcacg tcgaagcgca gcgccgtcat
     8221 ggtctcgttg aacagcctgt cccaagcccc gcgcgcggtg accgactttt cgtggaacaa
     8281 ctgctctagc ttgtcctcca gctgatacgg cttctctttg cgcaagtctt cgagccacgg
     8341 ccggaagtgg ccgagctctg cgcccgtcat cgccgtttcc agcgcggcgt cgtcgagccg
     8401 gttcagctcg agcgggaaga acagcagctt ggaggaaatc gccgtgatcc tctcctggat
     8461 gtcgccgtaa aacttctggc gcgcagggtc ggcggtatta gcggagtagc gcaggctcgc
     8521 ataggacacg atgcggccaa gcaggtcgtt caacgcctcg tagctgcgca ccgcctcggc
     8581 caaggccgca cccccgttgc cgccgtccag aagggccgca agcttgccgg cataacgcgt
     8641 atgcatgtcg tcggcagccc gctcggcctg ctcgaggtca ttcttgagcc tcggtgattc
     8701 gggagcgtca tacagcgcgc ccagatccca ttcgggtaac tcaccgagtg tcttttggga
     8761 ttttgccgtc tcggccgcgg caaagacggg gagcgggtgc cgaaggggga agttgcgcaa
     8821 catcgattca agaacctcgt tagcagtggt caacacctag tgtgggaacg ccatagcgcc
     8881 cctgcaaggc gcaaagaaga cgcttgtttt ggcactccca atcacggttc agcaagaact
     8941 tcaccaaaat cgaactgcca taaagaacat gtttaccctt gtggcgcact gttttggcac
     9001 agcagggggc cgctaagggc caagtgttcg tgccttaagc gctcctctga ctcgttagtg
     9061 tttgagtaac cagtgagtat tacgtcatgc caaagcgcgt cctggtcgtc gatgatgacc
     9121 cagcacaacg tcgtattctg gaagagacca tcacgcggtt cggctaccac gtcgaaactg
     9181 ctgagggcgg cgatgctgct cttgccgcgc tgaaggctga taccgcaggc gatatctgcc
     9241 tcctccttct cgacctcgtc atgcccgaga cagacggcat ggcggtgttg tcggccatgc
     9301 ggtccttacc gcgcaaagcc ccgaccatcg tacaaacggc tcacggcagc atcgatgcgg
     9361 ctatctccgc catgcgtgcc ggcgccgtcg atttcgtggt gaagccggcg agcccggaac
     9421 ggctcgaagt ctccatcaag aacgcgctga agatcgaggc cctggcgggc gagatcacgc
     9481 gcatgaaggc cactgccaac ggcgatcttt ccttcgccaa cctgattggc agcgacacca
     9541 tgaagcgcgt catcgaactc ggcaaccggg ccgccaagtc cagcattccc gttctcatcg
     9601 aaggtgagtc gggcgtcggc aaggaactga ttgcccgcgc cattcacggc gagagcgccc
     9661 ggcaagccaa gcccctcgtc acgctcaatt gcggtgcgat ccccgagaac ctggtcgaga
     9721 gcatcctgtt cggccatgag aagggctcct tcaccggcgc gaacgagaaa cgcatcggca
     9781 agttcgtcga agccgacggc ggcacgctgt tcctcgacga gatcggcgaa ttgccgctcg
     9841 acgctcaggt gaaactgctg cgcgcgctgc aagaaggcga gatcgacccg gtcggatcca
     9901 agcagtccgt gaaagtcgat ttccggctca tctcggcgac caaccagaac ctgatcacca
     9961 tggtgcaaga gggccgcttc cgcgaagacc tctactaccg gctcaacgtc ttccccattt
    10021 ggatcccgcc cttgcgcgag cgcctggagg acgtgcctga gttggtgcgc cacttcaccg
    10081 cgcgctttgc cgccgaggaa ggcaagcata tcgacggcat cgacgccgag gccgccgcca
    10141 tgctcgagcg ctacacctgg cccggcaata tccgtcagct tgagaatgcc gtgttccgcg
    10201 ccgtcgtgct ggccgacacg ccaatgctga ccgtgaacga attcccgcag atcgcggcac
    10261 acgtcgaagg ctatgcagtg actgttccgc ccgcgccggc accgaaagat ccggcaccgc
    10321 acatcgaggg cccggtcatg ctcggacagt cggccaccgc gcccgcgacg attcacgtgc
    10381 cgtcgggcaa cggcaaggac agcgtcggca ttccggcgct cagtgacagc ggcgacatcc
    10441 gctcattgga ggccgtcgag gccgacatga tccggctggc cttcggccgc taccggggcc
    10501 gcatgacgga gatcgccaag cgtctcggca tcggccggtc gaccctttac cggaagatgc
    10561 gcgagatcgg aatcgaggcg cggcccaact aatcgggaag aggccgcgca cggggacaag
    10621 acaaggaaag cggggagagg gagcatgacg acgaggccag tccacgggat cgcattgacc
    10681 ttgctgttgg ccgcgtcgcc ggccgtcgcg gtcgagcgag acgcatccga cgccgacact
    10741 gtcgcgatcg aaatgccgga gccgaagtcg caagcgcggc tgtctgcacc aggcagcgtt
    10801 tccgagacac ctgaagcgtc tcggacatcc gagacaaacg aagttcccaa gacaccggcc
    10861 gtcgccgaaa cccggccgtc aacgaccgag ccaatccggc gcgctgttca gtcccgtctg
    10921 tcgcagtcgt tcaccgcgac gtccacgcag aagaagaccg agaagggcgc tctggcggag
    10981 tactacgcca caccgaacca gaaacttctc tgggtcgacg agacgggcct caccgcgcgc
    11041 ggcaaggcgg ttatcgccga gatcgaacgc gcggacgatt acggcctgcg cgcgtccgac
    11101 tacacacttc cgaacatggc gggttttgag acgagcgcga gcgacgcgac ggagaaactt
    11161 gccgacgccg agatcgccat cagccatgcg gtcctcgact acgcgcacga tgcgcgcggc
    11221 ggcaggatca aacccagccg catttcgcgg aacctggatc cgacgctgaa cctgccgaag
    11281 cccatggagg taatcgagtc tctctccttc cgctccgacc cggccgctta cctgcgcagc
    11341 ttccagccgc agcacccgca gttcgagttg ctccgcaaga agctggtcga gctacgcggc
    11401 ggcggccagc aagcggcaaa acctgaaaag cccaaggtca agatcccagc cggacagacg
    11461 ctcaagctcg gcattgcgga cccgcaagtc gcgctgctcc gcacccgtct cgacgtaccc
    11521 gcgggcgaca accccgagat gtacgacgag gccctggctg aggcggtcaa acgcttccag
    11581 gcggaacaca acaccgcgtc tgacggcgcg gtcggccccg gcacgcggcg gcttctcaat
    11641 aatccgagct tgcgcgacat gggcagcccg gcgcagatca aaaagattct cctgaacatg
    11701 gagcgttggc gttggttgcc gcaggaccag ggcagtttct tcgtcaccgt caacgtgcct
    11761 gagttcacgc tgcgcgtggt caaggacgat gagccgatcc acaccagccg cgtcgtggtc
    11821 ggcaagctca agacccagac gccgattttc tccaacgaaa tgaagacggt cgtgttcggt
    11881 ccctactgga acgttccaac ctcgatcaag gtcggcgaga tccggcctta tgtccgcccg
    11941 accggcggct ggttcggcgg cggttggaat acgtcggtct tccaacgcca cgatctacgc
    12001 gtcaaatttg gtggacggga ggtcgacccg gcatcgctcg attggaaccg cgtcgacatt
    12061 cgcaagctcc acctctacca gcccccgggc ccgcgcaacg tgctcggccg cgtgaagttc
    12121 gtgttcccga acaaacacga cgtctacatg cacgacacgc cgcaaaagca tctcttcgcc
    12181 aagcgggttc gcgccgaaag ccatggctgc atgcgcgtgc agaaccccga ccagctggcc
    12241 gcggtaatcc tgaagcacga caggagctgg gacgacgcgc gcacgctgtc tgcgttccgg
    12301 tccggctaca accagcatgt cggcctcaac cgcaacgtgc ccgtctacat cacctacttc
    12361 accatgcgcg tgaacgagga cggctcgatc accacgtttg gtgatctcta cggccatgac
    12421 gggcgcatgc agaccgcgct cctggacggc cggaccgcct atgccgaggc ggacgacggg
    12481 atcatcaccc agtcaatccc gagacgccgg cgctcggcac cgtcgagaga ccggtgggag
    12541 cgcctgctcg acttttaacg aaatcgccgc cgccagcacc taaagcagcc acgggcccgc
    12601 attcgagcgg gccgtttctt ttgaaattct cttgcgaatt cagcctatta cccaaattta
    12661 accacccccc gtgatgcgcc ggcacggtta agacctttgc cttgtttttc gctcatttta
    12721 tcggaatact gttgcttatc ggcctcacct atcaggcagg ggccggaggc accaaaagag
    12781 cctaaaggct tggtgttact ggggaaagtc ggctgcaaac gccggcgagg gacgtttacg
    12841 gaaagacggg gatgcgcatt cgggggatcc atgcacgtcg cgccatgatt ggcgccggag
    12901 ttctgctcgc agcacttggc gcggccgtgg cgccgaatgc cttcgccagc gacacccgca
    12961 ccctatccct ctacgaaatc cataccaaag agcgcctcac cgtcacctat aagcgcgatg
    13021 gcgagttcga cgacgaggcc cttgaaaagc tcaactattt catgcgcgac tggcgtacgg
    13081 acgaaccgac caagatggat cgggagctga tcgatctcat ctggaccctg cacacccagc
    13141 tcggctcgca agagcccatc cacctcatct ccggctaccg cagcgccaag accaacgcca
    13201 agctccggcg cagaggcggc ggccaggcca ggaaaagcca gcacatccaa ggcagagccg
    13261 ccgatattca cttcccggac gtgccggtga agaagctgcg cgcctccgcg ctcatccaag
    13321 aggtgggcgg cgtcggctac taccccacgt caggtattcc cttcgtccat gtggacacgg
    13381 gccgcgttcg catgtggccg cgcatgccgc gcctcgagct cgccgcgctc ttcccggacg
    13441 gcaagaccgg atatgtcccc cgcgacggac ggcggattac caagaaggac cacgagcggg
    13501 ccctcgccaa aggcatggtg aacaagacct tgcttgccgc cgccaacccg gccacgccgc
    13561 cgccaacgcc gaagccgcaa cccatcatgg cgtccttcac accgccgatg gcgctggcac
    13621 ccgccgacaa gcccgccacc ccagccccgc aattggccag cgcgaacgcc ggcggcggtg
    13681 ttctgccttg gctgcagtcg cgcctcagcc ctgagcccaa gcgcccggcc gtgccgacct
    13741 acgagaagac gcaagttgtc gggccgccta aggtcgacga cgatcatccc gacgagctca
    13801 gctacctgcc gttcgagacg gcaagcctga tgaccgacac ttcggttgcc cgcaatccgg
    13861 agatcgcgcc gctcagccat ccggagcaga aagacttggg ctatctcttc gaggacatgg
    13921 accgcccgac cgccttcaag ctccgccgga cctcaggcta tctcggtctt gccagcgtgc
    13981 agacgttctc cggccgcgcg gtgaagagcc tctatgcggc gatcgacgcg caaaccgggc
    14041 ccacccgctt ggcgcagcgt tagcctctaa gccgccgcct cggtcgccgc agcgcccgcg
    14101 tcagaacccg cctcgccgtc catgccgagc gcatggagat aggtcgtaag gaccgcatcc
    14161 tcttcgcgcc gcttgttgtc gtccaacttg cgtaaggaga tgaccttgcg aagcgccttg
    14221 gcgtcgaagc catttccctt ggcctcggca tagacctcgc gcaaatccgc ggcgagcgct
    14281 gccttttctt cctccagccg ctcgatacga gacacgaagg cacggagctg atctttggct
    14341 gatgactgaa gcgacacgtt gggctcctca agcgtttttg tgatgtttcg atgaagacaa
    14401 gcttggggcc gaatcgcagc gtccagcaag gcgcaaggcc gagcttttcc cgcttatcca
    14461 aagccagagc ccgtcccgtc gtgccgttgg gcgggagatc gtaaattact gataatacta
    14521 tgctttttga ggtatcagga acgctcggtc tggaacttcg ccttccagtc ctcgtacggc
    14581 atgccgtaga tcaactcccg agcctcgtcc ttggcgagcg gcacgccgcg ctcgtctgcc
    14641 gcctccagca cccaagtcga caagcagttc cggcagaagc cggcgagctc catcaagtcg
    14701 atgttctcaa cgtcagtgcg ctcgcgcaaa tgctcgacca gccgccggaa ggccgccgcc
    14761 tcgatctcgg ttttcgtcgt ctcatccatt tgcgtcgtcc ttcgccttgc agagccctgt
    14821 gttggaccca tggtgttcga tggtgtgcag accaggtgcc gcgttcagca catcggcaat
    14881 cgttcgcgcc aaatgcgccg cccagatccc ctggccctcc tcgctcagga tcaaatcctg
    14941 gcgtacctcg atgagggcat gggcaagacc gcgcgacgtg gcatggcgat agagggtgtc
    15001 acccttcagc tgccccgagt acggctcgtt gtcgccgatc tctacatcgg gaatggtccg
    15061 caaggcctcg atcagcggaa ccggcatacg agggtccttg tcccacagca ccgcggctgc
    15121 ccacggcctc ggttttccct tccaggcctg ggtgaagctg tggatggaca cgatcaccgg
    15181 cggcttgccg gcggcaatcc ctgcgtccac cgcccgctca actgccgcgt gatagggccg
    15241 gtagaattgg tcgagacggg cctccatctg ctccgcgctc aaaccgacat tccccgggac
    15301 catgaggccg tcggacaact gcatgatcag ggtcgggtcg tcgaggccgc gattgggatc
    15361 gatcaggagc cgggagaacc ggctcaagac cgcgggcgcg ccgaccatcg tggccagcat
    15421 ttccgccacg ccctcggcgc ccagatcgac ggccatatgc cggtggagat cctcgtgcct
    15481 cagccctagg tcatggtacc catccggaat gcggttctgg gcgtgatcgc agaggatcag
    15541 caacccaagg cgcgcatccc cttcaatggt ccggtatggt ttttcgccca aatcgtcttg
    15601 tacgcagatg tccagtctca actccccggc gagcaatcct cgcgttcttc taaacttcca
    15661 ccgcacccct tgccgccgca acaacgaaaa ggcaaaaagc cccggctgcg acccatttac
    15721 gcaaagcttt caacggtgtt ctatgggtcc agatcggact tataatcgcc gccggtcggt
    15781 cagagaaatg caaccaattc aatgctatct tctgaattcg ttaaccgcga attcgggcat
    15841 gggaacctaa tgcttgcgaa caagaacatc cgcaatccag cgcggacctt taccacagcc
    15901 tggtctcggt ggggcatcgc cgcgagcgtg gccctcggcc tgacgacggg gttcgacgtt
    15961 gcgcccgcgc acgccgacct caagctgtgc aacacgaccg caagccgcgt cggcgtttcc
    16021 atcggttaca aggacaccga aggctgggcc agcgaggggt ggtggaatat cgcctcccac
    16081 acctgcgaga ccctgctgaa gggtgttctg atcggccgct tctactacat ccacgccatt
    16141 gactacgatc gcggcggcga atgggccggc gcgctgttca tgtgcacgga tgacaagtcc
    16201 ttcacgatcc gcgacaccaa ggactgcgag aagcgcggcc acaaacgtac aggttttttc
    16261 gaagtcgata cgggcgagga acgggactgg acagttcgcc tcaccgatcc tgatggcgaa
    16321 gcaaagcccc aatgaggcga aatcgacggg tcaagatcgt cgccacgctc ggtcctgcct
    16381 ccaccaacga agaaacgatg cggcgcctgt tcgaagcagg cgccgatgtg tttcgcgtga
    16441 acatgagcca cgcgcagcac gatgccttgc gcgagatcca cggcatcgtc cgcaaactcg
    16501 agtccgagta ctcgcggccc attggcattc tggtggactt acagggaccc aagctccgca
    16561 tcggcaagat cgactccgga tcgatccgcc tgactcaagg tgacattctg agcttcggcc
    16621 gcggcgagga cgtgggcaat ttgggccgca ttcagttgcc ccaccccgag atatttgaat
    16681 ccgtggagaa gggccacacg ctgctgctcg acgacggcaa gttgaagctg cgcgtggtcg
    16741 aggccagcga cgtgcgtatc gacgcagaag tgctcagctc cggcctgctg ggcagccgca
    16801 agggcatcag cctgcccgat accgtgttgc ccatgggcgc cttgaccgac aaagatcgcg
    16861 cggacatcga atgcgccatg tcgttgggcg tcgcgtggat ggcgctgtcc ttcgtgcagc
    16921 ggccagagga tattgccgaa gcgcgcaagc tcagccgcgg ccgcacctcc atcatggcca
    16981 agatcgagaa acccactgcg atcaaccact tgaaggagat cgtcgatctc gccgacggga
    17041 tcatggtggc acgcggcgat ctcggcgtgg aaatgccggt cgagaaagtc ccggggcttc
    17101 agaagcaaat cagccgcgcg gcacgcaatg ccggcaaacc ggtggtcgtc gccacccaga
    17161 tgctggagtc gatgatctat gcgccggtgc cgacccgggc cgaggtctcc gacgtcgcca
    17221 cggcggtgtt cgaaggggcc gatgcggtca tgctctcggc ggaatcggcg gtcggcaaat
    17281 atcccgttga tgctgtcgcc accatgaacc ggattgccgt cgaggtggaa cacgatccgc
    17341 tccatgccgc catcatccat gcccagcgca tcgatccgac ggcgacggac gccgacgcga
    17401 tctccgcggc cgcgcgcacg gtcgccgaga ctctcaatct cgcggcgatc gtctgctaca
    17461 ccgcatcggg ctccacatcg ttgcgcgccg cccgcgaacg gccgcaacag ccgatccttg
    17521 cgctgacgcc aattcccgcg acgggccgca agctggcaat cgtttggggg cttcactgcg
    17581 tgctgacgga cgacccgcgc aatctggacg acatggtcgc caaggcctgc cgcattgcct
    17641 atcaggaagg cttcgcgctt ccgggcgagc aggtcatcat caccgccggc gtccctcttg
    17701 gaaagtcggg cgccacgaac ctgctgcgca ttgccgaggt ggacccaggc accgacgagt
    17761 agcttgggtg ggacttagat gtctttgact tagatatctt cgacttagat gtcttggcct
    17821 tcgacttcgc gcttaagcgc gtcgacgccg gcctgcgcat cctccaagaa gggattgacc
    17881 gcaagcgccc ggcgataggc ttcgagcgcc tccttcttgg caccgagttg ctccagcaat
    17941 agcccgagat cggcaatggc gcggtagtgc ttggcgtcca gattgagggc ccggcgaaga
    18001 tcggaaaggg cgcgctcggg cttgcccttc agcacgttca ccttcgcgcg cagataccaa
    18061 gcctcggcct cgtccggcgc gatgtcgaca acggcatcga gaaccgcaag cgagagatcg
    18121 atctccgctt ccttggcaaa tcgggccgcg cggctcatca agagatcaac ggtgtcgctt
    18181 ccggacacca tccacaaatc ctcgatagct tccatgatcg gctcagccgc ggtcgcgctc
    18241 cgcgccgcgc ccagcttctc ataaagctcc gccagcagtt tcggccgctg cgccggatcg
    18301 aacggcgcgc cgccaagact gggatcaaga ctgggatcaa gatcaagacc gagttctagc
    18361 tcatcctggg aaccgtcggc cgattggccc gcgccgggct ccaagccctc caggcccgga
    18421 agatcgagcc cctccaaggc tgggtcgtcc gcaggcgggc gcgcctcgtc ggtctcctcg
    18481 tcgaagtgct caaagaattg cgccaacacg agcggtgggc tggcaaaggc ggtgcccgcg
    18541 aaggacaccg aaagcgccaa tacgaagagg cccgcgaaac gtcttactgt ggacatgctg
    18601 aaaaaatacg gggcccgcta gggccccgtc aaagcaattc gtcctgaaag tcccagaaga
    18661 cgcctcctgg gctggaaccg cgcgcttagc cctggcgggc cttgaagcgc cggttggtct
    18721 tgttgatcac gtaaagccgg ccgcggcggc gcaccacccg gttgttccga tggcgcttac
    18781 gcagggccct cagcgaattc ttgatcttca tgacacgcca tccattcggc tgaataaagt
    18841 ccaggcggcc ctcgtcaaaa ggccgccctc gaaatttggc cggaccataa gaaggcaggc
    18901 gatgcctgtc aaccaacggg ccggaagcga aaaacgcccc cgcgaccgtg gatggccgcc
    18961 ggggcgttag gtcgaaaaat gccttggagc gcctagtctt cctcgacctt cttgatgacc
    19021 ttgaagtcct tgtcgagata aacttcgtac tgctttctgt cctcgcagat gacatcctcg
    19081 gcctcgtagc cgctcttctc cgcctcgatc tcgctgtcag caactgtgca gcccatcgcc
    19141 tgaatggcct tctgcacgcc ctcgatttcc tgctcggtga gcgccctgtc gtcggcaaag
    19201 gcggacgtcg ccatggcaaa ggtaagagca aaagcagccg ctagaatttt catgtgtgct
    19261 acctccattg aaccaaaccc tcatatgggg aaccggacaa tcgggcggga gactttcgta
    19321 ccgcgacttt cctgaacatt tcttaatctt aggggcagcg cgtgagcccc cccacggaac
    19381 caagaacggc cccgatgcgt ttggcatggg agaggtcgtt aggtccgggg gtctcccatg
    19441 cataccgaca cttatctttt cgtggtttgt gcacgccgca atgcgagcag cattttcggg
    19501 gcggcatcga accctgtgat gcggaacggc aaactcctct gtttcaagac cgaggccggg
    19561 gcgcgcgccg aacgcgatcg gctgaccgcc cgcttgggcg aggcccatgt gcgctacagc
    19621 gtcaggccgg tccatgttca gcttaaaccg ccgcacgagg ccgccaaggt agaggctgcc
    19681 ccgccctcac gcgccatgcc tatgcctgag gccgcgtagc ggggactctg gctttcgcca
    19741 aaacaggctc ctatcctgaa cgccatgcgt tcttggatca ttaggggcct cacacaatgc
    19801 agcgatatcg cctcatggcg caaaacgcac tctgcgccat cgccacgata ttcttcacag
    19861 ccgtgtttgc ggccggcgtc gcgatgagcg ctgacatctc gcttgcagga tcggaatggg
    19921 gatttgccaa cgagacgggc aaaccggccc gctacatcca gttcgccgag gaccaggcca
    19981 gcggcaatct cgggtgcaac cgtttcacgg gcggatattc cttcaaagac ggcgaactga
    20041 acttcaggcc cttggcgtcc acgaggatgg cctgcgcgcc cgaggccatg aaacgcgagc
    20101 aagagttcgc cgcccttctc tccaaagttc gctttgccga agcgacccac ttgaagctcg
    20161 tcctcaagga tgcggatggc gaagtcctag ccgaactggt gcggcgcgac cccgactagg
    20221 cggcctcgcc gcgttccggc cttgccgcgt taacgactcg gtaagaaatg gagcgatagc
    20281 cttttggggc tcaaccgaac gggaagttaa caaaacaggc gtttttcgcc cggccaggaa
    20341 agggctcctg gtcgggcttt tatttgcctt cctaccaaaa cactggtgcc aggagcgtca
    20401 ccccggcggc aataatcgcc gcgccgagga aggtgagcag cgcccctgcc cggatcatct
    20461 gcgggatcgt gacataaccc gtcccgaaca cgagtgtgtt gggaggcgtg gccacgggaa
    20521 gcatgtaggc ggacgacgcc gcgagcgcga tgggaaagac gatcagcagc ggcgcaatgt
    20581 cagacccgag cgccaagctt ccggcgatgg gtagaaacgc cgcggccgtc gccgcgttgc
    20641 tcatccactc cgtgagcaat gcgaccagca aggctacgcc gaaaacgagc acgaccaggg
    20701 gcatgctgtg gaggaaggac aggccattgc ccatccacgt ggccagctgc gagacttcga
    20761 tcgccttggc caaactcaaa ccgcccccaa agagtatcaa gacctcccat ggcactttgc
    20821 gcacggccat ccggccgagc agaaaggtcc gctctcgcca attggccggc acaacgaaga
    20881 gcgcaatcgc gccggcgatg gcaatccccg tgtccgtgag gccagctcca agccaatcgt
    20941 gaaccagcgg gcgaaagatc cacgcggcgg caacaacggc gaatatgatc gcgacccgcc
    21001 gctccggcac gctcatgggt cccatctcgc gcaacatctc gccggccaca tcggtcttcg
    21061 cctcaccgag actgt
//
LOCUS       DBNHVU010000118         2964 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_416995, whole genome shotgun sequence.
ACCESSION   DBNHVU010000118 DBNHVU010000000
VERSION     DBNHVU010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_416995"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="AC16Y7_04690"
     CDS             complement(<1..713)
                     /locus_tag="AC16Y7_04690"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5708712.1"
                     /translation="MRRLDDKIALVTGGGAGIGRAICETFAREGAHVVVADIDGDAAK
                     ETADAIIKANGAASAQTVDIADTAQVKALMDDIKQTHGRLDVLVNNAGVGARADFRHL
                     SDEDWEKVWAVNLDGTVRCAREAFDLMRASGKASVINLSSVMSAKHTRQMAVYSATKG
                     AVSALSRSLAVEYAPYGIRVNTLLPGYVETALIGRFLSNPMIAKALLTQTPLRRFGKP
                     EDIANAALFLASDEAAYITG"
     gene            complement(700..2094)
                     /gene="gor"
                     /locus_tag="AC16Y7_04695"
     CDS             complement(700..2094)
                     /gene="gor"
                     /locus_tag="AC16Y7_04695"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HIR5708713.1"
                     /translation="MSEYDYDLFVIGAGSGGVRAARLAAALGKRVGIAEEYRIGGTCV
                     IRGCVPKKLLVYASRYGGEIEDARKFGWDYEDLQFDWPTLIHNVHREVDRLNGAYTRT
                     LENSGVDRFLTRATVDGPHGVRLADREAPISADYILIATGGSPSMPDVPGKEHLISSN
                     ECFQLEALPDSIVVIGAGYIGMEFASIFAGLGVGVTVLHRGEQVLRGFDMDLRDGLAE
                     AMRDRGVDLHMNADIAAAEKDGGGYRVALKDGSSVCASLVMAATGRGPNTKGLGLEDA
                     GVELGRNGNVVVDEYSKSSVDSIYAAGDVSDRVALTPVAIREGTAFVETVFEGRPTVM
                     DYTFIPTAVFTEPEIGTVGLTEEEARKKYREVDIYKSSFRPMRATISGRDETVLIKIV
                     VDAATEKVLGVHLLGPDAAEIAQMAAISLKLGVTKPQLDSTMALHPSIAEELVTMRQK
                     WTPPEPIKGSDAAA"
     gene            complement(2103..2822)
                     /gene="rpiA"
                     /locus_tag="AC16Y7_04700"
     CDS             complement(2103..2822)
                     /gene="rpiA"
                     /locus_tag="AC16Y7_04700"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HIR5708714.1"
                     /translation="MADSAAKVDEFKANAAKAAIELVKPGMRLGLGTGSTAYHFVEAV
                     GGLVRNGLEVICVPTSEATHAHAEKLGIPLSSLDETPQLDLTVDGADEIDDQLRLIKG
                     GGGALLREKIVATASDRMVVIADDSKVSKTLGSHPLPIEVVRFGLRATMRLIEALAAE
                     AGCEGEIRLRPGQGDAPYVTDQGNLIVDCAFHKIPEPEVLAFAMKRVPGVVEHGLFLG
                     LADIAIIGGADGVQAIHHPGA"
     gene            complement(2822..>2964)
                     /locus_tag="AC16Y7_04705"
     CDS             complement(2822..>2964)
                     /locus_tag="AC16Y7_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5708715.1"
                     /translation="VNEGIISSELAPFGGVKESGQGREGSHHGIEEFLELKYVMMGGL
                     EG"
BASE COUNT          550 a         1002 c          858 g          554 t
ORIGIN      
        1 ccggtaatat aggccgcctc gtcggaggcg aggaacagcg ccgcgttggc gatgtcctca
       61 ggttttccga aacgccgcag cggcgtttgc gtaagcagcg ccttggcgat catcggattg
      121 gacagaaacc gcccgatcaa ggccgtctcc acataacctg gcagcagcgt gttcacgcgg
      181 atgccgtagg gggcgtactc cacggcgaga ctccgcgata gggccgacac cgcgcccttg
      241 gtcgccgagt agaccgccat ttgccgcgta tgtttggctg acataacgga tgagagattg
      301 atcaccgacg ccttgccgga tgcgcgcatc agatcgaaag cctcgcgcgc gcaacgaacg
      361 gtgccgtcga gattgacggc ccatactttc tcccaatcct catctgacag atgacggaag
      421 tcagcccgcg ccccgacgcc cgcattgttg accagcacat cgagccggcc atgtgtctgc
      481 ttgatgtcgt ccatcaaggc tttcacctgc gcagtgtcgg cgatgtcgac agtctgtgcg
      541 ctcgccgcgc cgttcgcctt gatgatggcg tcggcggtct cctttgccgc atcgccgtcg
      601 atatcggcga ctacgacatg cgcgccctcg cgcgcgaacg tctcgcagat ggcacggcca
      661 atgccggcgc caccgcccgt tacaagcgcg attttgtcgt caagccgccg catcggagcc
      721 ctttatcggt tctggcggcg tccatttctg ccgcatggtg acaagttctt cggcgatact
      781 tggatgaagc gccatggtgg aatcgagttg cggcttggta acgccgagtt tcagagaaat
      841 tgcggccatc tgcgcaatct ccgccgcatc gggtccgagc agatgaacgc cgagaacctt
      901 ctcggttgcg gcgtcgacaa cgatcttgat gagcaccgtc tcgtcacgcc cgctgattgt
      961 tgcacgcatg ggccggaagc tcgacttata gatgtcgact tcgcggtact tcttccgcgc
     1021 ctcttcctcg gtcaacccaa cggtgccgat ctccggctcg gtaaacaccg ctgtcggaat
     1081 gaacgtatag tccatcaccg tggggcggcc ttcgaacacc gtctccacga acgccgtccc
     1141 ctcgcgaatg gccacgggcg tcagggcaac cctgtcgctg acgtcacccg ccgcgtagat
     1201 gctgtcgacg gaggacttgg agtattcgtc gacgacaacg ttgccgttcc ggccaagctc
     1261 cacacctgcg tcttccaagc ccagcccttt cgtgttgggc ccacgcccag tggccgccat
     1321 caccagagac gcacaaaccg agctgccgtc cttcagcgcc acccggtagc cgcccccgtc
     1381 tttctcggca gcagcgatat ctgcgttcat gtgcaaatcg acaccccggt cgcgcatagc
     1441 ctcggccaag ccgtcccgca aatccatgtc gaagccccgc aagacctgct cgccgcgatg
     1501 gagcacggtc accccgacac cgagtccggc gaagatcgag gcgaactcca taccgatata
     1561 gccggcgccg ataacaacga tgctgtcggg aagcgcctcg agctgaaagc actcgttcga
     1621 actgatgaga tgttctttac cgggaacgtc cggcattgac ggagacccgc ccgtcgcaat
     1681 caaaatgtaa tcagccgaga tgggcgcctc gcgatccgca agacgcacgc cgtgcggacc
     1741 gtccaccgtc gcgcgcgtaa ggaagcggtc gacgccggaa ttctccagcg ttcgcgtata
     1801 ggcgccgttc agccggtcga cctctcggtg cacgttgtgg atcagcgtcg gccaatcgaa
     1861 ctgcaggtcc tcgtagtccc agccgaactt gcgcgcatct tcgatctcgc cgccataacg
     1921 gctggcatag acgagcagct tcttcggcac gcatccgcgg atcacgcagg tgccgccaat
     1981 gcggtattcc tcggcaatgc cgacgcgctt gccgagcgcc gcggcgagcc gcgcggcccg
     2041 cacgccgccc gatccggcgc caataacgaa gaggtcgtaa tcgtactcag acacggatcg
     2101 cctcatgctc ccggatggtg gatcgcttgg accccatcgg caccgccaat gatcgcgatg
     2161 tcggccagcc ccaagaacaa gccatgttcg acgacgccgg gaacccgctt cattgcgaag
     2221 gccaatactt cgggctcggg aatcttgtgg aaggcgcaat cgacgatgag gtttccttga
     2281 tcggtgacat acggcgcatc gccctgcccc ggccgaagcc ggatctcgcc ctcgcagccg
     2341 gcctcggccg ccaaagcctc gatcagccgc atggttgccc gcaaaccgaa ccgcaccact
     2401 tcgataggca gcgggtgcga cccgagcgtc ttcgaaacct tgctgtcgtc ggcaatcacc
     2461 accatacgat ccgacgccgt cgccacgatc ttctcgcgca gaagcgcgcc ccctccgcct
     2521 ttgatcagac ggagttggtc gtcgatctcg tcggcgccat cgacggtgag gtcgagttgc
     2581 ggggtctcgt cgagagacga cagcggaatg ccaagctttt cggcgtgcgc gtgcgtggcc
     2641 tcggacgtgg gcacgcagat gacctcaagg ccgttgcgca caagcccgcc gaccgcctca
     2701 acgaagtggt aggcggtcga gcccgtgccc aacccgagcc gcatgccagg cttgaccaat
     2761 tcgatggcgg ccttcgccgc attggctttg aattcgtcga ccttcgccgc gctgtccgcc
     2821 attagccttc gagccctccc atcatgacgt atttcagctc caaaaattct tcgatgccat
     2881 gatgggagcc ttcgcggcct tggcccgact ccttcacgcc gccgaacggc gccagctccg
     2941 atgagatgat gccttcgttg acgc
//
LOCUS       DBNHVU010000119        12178 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_788418, whole genome shotgun sequence.
ACCESSION   DBNHVU010000119 DBNHVU010000000
VERSION     DBNHVU010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 12178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12178
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_788418"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="AC16Y7_04710"
     CDS             complement(<1..1212)
                     /locus_tag="AC16Y7_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase family protein"
                     /protein_id="HIR5708716.1"
                     /translation="MRILGVSAYYHDSAAALIEDGRVIAAAQEERFTRRKHDAAFPKN
                     AVQYCLSEAQVPLSSLDAVVFYEKPFLKFERLLETYMTTAPRGFRQFLMAMPVWLREK
                     LFQKSLLAKELKEIDPDFDPEKLLFSEHHLSHAASAYFPSPFESAAILTLDGVGEWTT
                     TAAALGQGSRIELTKELQFPHSLGLLYSAFTYYTGFKVNSGEYKLMGLAPYGEPKFSD
                     KILDNLIDVKPDGSFRLNMSFFDYMTGFTMTNRRFARLFGEKVRGPEDRLTAFHMDIA
                     ASVQAVTEEVVLRLTRSLAAETGQPNLCLAGGVALNCVANGKVLRDGSFERIWVQPAA
                     GDAGGALGAALAAYHVEFAKTRENTDTLDEMSGAYLGPAFEADSVAERLTAAGAHFEV
                     CNDDELYERTAD"
     gene            complement(1236..1385)
                     /locus_tag="AC16Y7_04715"
     CDS             complement(1236..1385)
                     /locus_tag="AC16Y7_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HIR5708717.1"
                     /translation="MSFLSELMEFLLARKKYWLIPVVLLMVVFGGLLVMSQGSAVAPF
                     IYTIF"
     gene            complement(1392..1817)
                     /locus_tag="AC16Y7_04720"
     CDS             complement(1392..1817)
                     /locus_tag="AC16Y7_04720"
                     /inference="COORDINATES: protein motif:HMM:NF039992.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SxtJ family membrane protein"
                     /protein_id="HIR5708718.1"
                     /translation="MGKNFHESLSRGQTVQMGSNRSFGLVMGAACLVIAGIGYWFGSA
                     YWPVWTGAAAVFGALAMLWPSVLYPLNRLWFWFGLALHKVVNPLVMGVLFFVVITPIG
                     LLMRLSRKDPLGLETEPDAASYWAKRGSELQPGPMSKQY"
     gene            complement(1841..1917)
                     /locus_tag="AC16Y7_04725"
     tRNA            complement(1841..1917)
                     /locus_tag="AC16Y7_04725"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1881..1883),aa:Arg,seq:ccg)
     gene            2091..3752
                     /locus_tag="AC16Y7_04730"
     CDS             2091..3752
                     /locus_tag="AC16Y7_04730"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5708719.1"
                     /translation="MAKKKKIIVIGGGFGGVFAAKTLEERGRGLVDVELINNNNYFVF
                     QPLLPEVASSNISASDAVVPLRQLLTKVQVRQARVMGIDFKTKTVIVMQGARMTPVEL
                     KYDELVIALGTGVDLARIPGMADHALTMKDLTDAHMLRTHAIGCLETADVTTETPVKD
                     QLLSFVVVGAGFSGVETAAELRELIRNALPYYHNIKPEEIRMYLIEYAPRILPTFPAE
                     LAEYATKQLSKSGIEVLTGVGTESATATGVALTNGRIINSSTVVATIGNGPHPLVQTL
                     GLDMHWGRIRTDRFMQVPGQEHVWALGDAALIPLNDNPGEDPADYATQTAQFAVREGY
                     QLAGNVVAKLKGEELKPFAYKSKGSLASLGMSKAVADVYGIKLSGMFAWLLWRGFYLS
                     FLPGFVAKFRVGLNWMINAIMPPNIVQLRMRPPATRYIHFWRGDKVFEPGMLIDGFYT
                     VIKGSFKLTIDNPDTDEHFEKVFGPGEHFGERVLVKSELRTGLVQALEDGLLLFIAQK
                     DFTRFATAFPTLDAYFKDYIEETFGGPDKAFAPGNIDQSELEKTH"
     gene            3832..6780
                     /gene="polA"
                     /locus_tag="AC16Y7_04735"
     CDS             3832..6780
                     /gene="polA"
                     /locus_tag="AC16Y7_04735"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR5708720.1"
                     /translation="MTAAKTSQSAAGKPAGRGDHVYLIDGSGYIFRAYHALPPLTRPS
                     DGLPVGAVHGFCGMLWKLLRETGELAPPTHMAVIFDYSAKTFRSDLFDGYKANRPEPP
                     EDLIPQFPLVRDAVRAFNVACIEKEGFEADDLIATYALQAVGAGADVTIVSSDKDLMQ
                     LVRPGIVMYDTMKNKKIAEEGVLAKFGVPPSKVIEVQALMGDSSDNVPGVPGIGVKTA
                     ALLINEYGDLETLLARASEIKQNKRRENLIEFAEQARLSKTLVTLDTQTPLDVPLAET
                     AVQQPEPETLTAFMREMEFSTLLKRVAEGLGAPLPKGATKPSAVRRKKDDYDHPLRKS
                     PRGAAGPARDIDVQGGSPAELAIERASKLEEIPFDRTNYETVTDIARLQDLLKTAQFQ
                     GNLAIRVKLNTSDPMTGEIAGVALALAPGHAAYVPLSHRAGDGLDLGGEDVGPQAPLR
                     EALDLLKPILEDESVLKIVAGAKPDMVALARYGIALQAVDDPCLISYALDAGRADHAP
                     AKLAAARLGHTALTEKEVLGTGKSAIAFDLVEVARATEAPGEEADIAFRLWMLLKPRL
                     AAEQVTTVYETLERPLQPVLAAMERAGIKVERSALANLSGAFAQTIARLEDEIRDLAG
                     EDFNIGSPKQLGEILFEKMSLPGGRKTKTGAWSTDAAALEELAAEGHDLPRKVLEWRL
                     LAKLRSTYTDALPAYIHAETGRVHTRYVLASTTTGRLASHEPNLQNIPVRTKEGRSIR
                     KAFVAEKGKTLISADYSQIELRVLAHMADTPTLRQAFADGLDIHAMTASEMFGVPIEG
                     MDPAVRRRAKAINFGIIYGISAVGLGAQLGIPRGEAAAYINTYFERFPGIKDYMEAMK
                     AEVKRSGFVTTLFGRKVHYPEINTKNPSLRGNYERAAINAPIQGSAADIIRRAMIRMD
                     EALLDAGLSARMLLQVHDELIFEAPEDEAEKTMEVAQEIMEHAPEPALRLRVPLKVDA
                     RAAANWEAAH"
     gene            6808..7272
                     /locus_tag="AC16Y7_04740"
     CDS             6808..7272
                     /locus_tag="AC16Y7_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708721.1"
                     /translation="MRTAFALLVSCLIALPALALPAPMSEAELEEKSGIVALVRVMSV
                     TCTSIIKDKTTGEDLPGYIAKLRVIKAKKGDVRPGGEVLVTWRAKSQKLIGGWTVPYY
                     PGEEVWTHLTKRAGGATYASTWWNAKGDEVKPPETKELPTVVGKSALSDDQD"
     gene            complement(7269..7961)
                     /locus_tag="AC16Y7_04745"
     CDS             complement(7269..7961)
                     /locus_tag="AC16Y7_04745"
                     /inference="COORDINATES: protein motif:HMM:NF005458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HIR5708722.1"
                     /translation="MKNAVAIYHVAFEDAGTLGPVLEERGVELTYLQAGRDDLSPALD
                     ADLVLVLGGPIGIYEIERYPFLKEELALVEKLVKQGTPVVGICLGAQALAAVLAARVY
                     PGKQAELGWDTLTLTDEGKDSPLGVLEGLHILNWHGDTFDIPTGATRLASTDLTPNQA
                     FSYGPKVLALQFHVELPGRDMERWLIGHTLELAKNNVDLAQMRTETARYAPPTNEASR
                     ALFNMWLDNLSA"
     gene            complement(8062..9585)
                     /locus_tag="AC16Y7_04750"
     CDS             complement(8062..9585)
                     /locus_tag="AC16Y7_04750"
                     /inference="COORDINATES: protein motif:HMM:NF013206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR5708723.1"
                     /translation="MTKTKSVVAAGHALTASAAAEILADGGNAFDAAIAGVCMSFVAE
                     AVFASPGGGGFLMARQAGSNAIKLFDFFVETPLRRRPANEVSFFPIEADFGPATQEFH
                     IGLGAAAAPGIAQGLFAMHEAYARLPMKRLVEPAARAARQGFPLNSFQAYLFTVIAPI
                     LRECEGARAIFAANGKLMEAGETFRNDGLAETLEWLAEDGARLFVDGDIGQAIVAQSR
                     ELGGHLTEDDLKRYRVALRDPLLWRHGGATVALNPPPSAGGALIAFGLAYLGAHAEAG
                     GASDAEALQAAMTATNAARANHGEDLAARLADGALANEIAAAAQYPNGYRGTTHVSVI
                     DGDGNAAAVSLSNGEGDGFIVGNHGFMLNNMLGEEDLAFGDLDAWREGSRMSSMMAPT
                     IIMEPDGTVTALGTGGSNRIRTAILQVAINLLAHGMSLEDAVNAPRLHVERCGTTSFE
                     PGFPEETEAAFRAAGDKARAWPERNLFFGGVHAARWHETRGVEGAGDPRRQGVSQTV"
     gene            complement(9703..11508)
                     /locus_tag="AC16Y7_04755"
     CDS             complement(9703..11508)
                     /locus_tag="AC16Y7_04755"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HIR5708724.1"
                     /translation="MNVQSKIAPTTNKKVLAFVEEAKELFKPDSLYWCDGSQEEYQAM
                     LKALVDGGTAMWLNEEKRPNSILVRSDPKDVARVEDQTYICSESEADAGPTNNWFDPA
                     KMKETMAKLYDGAMQGRTMYVMPYSMGPVGSPIAGIGVMVTDSAYAVANMHIMTRVGD
                     AVWKALGDSADFVCGRHTVGAPLTSDKQADVPWPCNETKYISHFPETREIWSYGSGYG
                     GNALLGKKCHALRIASVKARDEGWMAEHMLILKLTNPEGDVKYMAAAFPSACGKTNLA
                     MLDPTIPGWKAECIGDDICWMKFGEDGRLYAINPETGFFGVAPGTSEASNLNAMKALD
                     KNCIYSNVALTDDGDVWWEGMTKESPAHTIDWLRRSWTPESGRPAAHPNARFTAPAGQ
                     CPVIAPEWEDPKGVPIDAILFGGRRASVVPLAVEAEDWKQGTFLGSIMASEKTAAAAD
                     AKGDIRRDPMAMLPFCGYHMGDYFAHWLKMGEKGGDKMPKIFYVNWFRKSPEGKFLWP
                     GFAENSRVLKWIFERCNGTTDAVDTPIGKVPTKDALDTDGLDVDAADLEELLKVDTKG
                     WKDELPMIKEHYETFGDKLPGGLKEELDDLEKRLG"
     gene            complement(11639..>12178)
                     /locus_tag="AC16Y7_04760"
     CDS             complement(11639..>12178)
                     /locus_tag="AC16Y7_04760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022083.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HugZ family pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HIR5708725.1"
                     /translation="DPRAAILIDGSGAAADPLQGARLTLSGKAGRVDDEVVRRRFLVR
                     HPEAAFYADFPDFSFWRLDLEGAHYIGGFGRIVDLSPGDLLLPQKGAETLLAAEPDIV
                     AHMNDDHADAIELYARAFAGRHPGPWRMIGIDPEGCDIALGVDGARISFEKSVLTPAD
                     ARKELVRLAEEARSAVNSS"
BASE COUNT         2308 a         3851 c         3849 g         2170 t
ORIGIN      
        1 gtccgctgtc cgctcgtaga gctcgtcgtc gttacagact tcaaagtgtg cgcctgcggc
       61 ggtgaggcgc tcggcgacgc tgtcagcttc aaaggcgggg cccagatagg cgcccgacat
      121 ctcatcgagg gtgtcggtgt tctcacgggt ctttgcgaat tccacatgat aggcggcaag
      181 cgctgcaccc agcgcgcccc ccgcgtcgcc ggctgcaggc tgcacccaga tacgctcgaa
      241 gctcccatcc ctgagcacct tgccgttcgc aacgcaattc agcgcgacac caccggcgag
      301 gcaaagattc ggctgtcctg tttcagcggc gagcgaccgc gtgagccgga gtacgacctc
      361 ttcggtgacc gcttgaacag aggcggcgat atccatgtga aacgctgtta ggcgatcttc
      421 tgggccgcga accttctcgc cgaacaaacg tgcgaaccgg cggttggtca tggtgaagcc
      481 ggtcatgtag tcgaagaagg acatattgag ccggaagctg ccgtcgggct tcacgtcgat
      541 cagattgtcc agaatcttgt cggaaaattt tggctcgcca tagggcgcca gccccatcag
      601 cttgtactcg ccggaattga ccttgaagcc tgtgtaatag gtgaaggcgg aatagagcag
      661 gcccagcgaa tggggaaact ggagctcctt cgttaactct atgcgactac cctgcccgag
      721 cgctgctgcc gtcgtggtcc attcacccac accgtcgagc gtaagaattg cggcgctctc
      781 gaagggcgat ggaaagtagg cgctcgccgc gtgactcaag tgatgctcgc tgaaaagaag
      841 tttctccgga tcgaagtcgg gatcgatctc tttcagttct ttcgcgagta gcgatttctg
      901 aaatagcttc tctcgcagcc ataccggcat tgccatgagg aattggcgga agcctcgagg
      961 cgcggtcgtc atataggttt cgagcagacg ctcgaacttc aggaacggct tctcgtagaa
     1021 gacgacagcg tcaaggctcg agagcggcac ttgcgcttcg cttaagcagt actggaccgc
     1081 gttcttcggg aatgcggcgt cgtgcttgcg gcgcgtgaac cgttcctcct gggcggcggc
     1141 gatcacgcgt ccatcctcga tgagcgcagc ggcactgtcg tgataatagg ccgagacgcc
     1201 aaggatacgc atgacttccc cttggggcgg gctggctaga atatcgtgta aatgaacgga
     1261 gcgaccgcag agccttggct catgacaagg agcccgccaa acaccaccat cagcagaacg
     1321 actgggatca accagtactt cttccgggcg agtaggaatt ccatcaattc agaaagaaac
     1381 gacatggctg atcaatactg cttgctcatg gggcccggct gcaattcgct cccccgctta
     1441 gcccagtagc tcgctgcatc gggttcggtc tcaaggccga gcggatcttt acgcgagagc
     1501 cgcatgagca agccgatggg cgtgattaca acaaagaaca gaacgcccat caccagcgga
     1561 ttgacgacct tgtgcagcgc caggccgaac cagaaccaca ggcggttgag cggataaagt
     1621 acgcttggcc acagcatcgc cagggcacca aaaaccgccg cggcccccgt ccatacgggc
     1681 cagtaggcgg agccaaacca atatccgatc ccggcaatga cgaggcacgc cgcccccatc
     1741 accaggccaa acgatcggtt cgagcccatc tgcacggtct gcccgcgcga cagactttcg
     1801 tggaaattct tgcccacagt gacaaccttc tcgtggcgat tggcgcaccc gtcaggattc
     1861 gaacctgaga cctctgcctt cggagggcag cgctctatcc agctgagcta cgggtgcgtt
     1921 gtcgacggga ccctagccaa agtgcccctt tgaagcaacg ccatggcggc gctgcctcga
     1981 tatcgggctt tggggccaac cgtgcaacct gccgcaggct gtcgcggtga cttgacgcgc
     2041 cggagcattg gcgtacctac tctttcgcaa cggaaaggct gggcaagcct atggcgaaaa
     2101 agaaaaagat cattgttatc ggcggcgggt tcggcggcgt gttcgccgcg aagaccttgg
     2161 aagaacgcgg gcgcggcctg gtcgacgtcg aactcatcaa taacaacaac tacttcgtgt
     2221 tccagccgct cctacccgag gtggcctcgt cgaatatcag tgcgtccgac gcagtggtgc
     2281 cgctgcgcca gctcctgacc aaggttcagg tgcgccaggc gcgggtcatg ggcatcgact
     2341 tcaagaccaa gaccgtgatc gtcatgcagg gcgcgcggat gaccccggtc gagctcaaat
     2401 atgatgagct ggtcatcgcg ctcgggaccg gcgtcgacct ggcgcgcatc cccggcatgg
     2461 ccgaccacgc gctgaccatg aaggacctca cggacgcgca catgctgcgc acccatgcga
     2521 tcggttgcct ggagacggcg gacgtcacga ccgaaacgcc ggttaaggat cagcttctgt
     2581 cgttcgtggt ggttggtgcg ggcttttccg gcgtggaaac ggcggcggag ctgcgcgagc
     2641 tgatccgcaa cgcgctgccc tactatcaca acatcaagcc cgaagaaata cgcatgtatc
     2701 tcatcgaata cgcgccgcga attctgccga ccttccccgc tgaacttgcc gagtacgcta
     2761 ccaagcagtt gagcaagagc ggcatcgagg tgctcaccgg cgtcggcacg gaatcggcga
     2821 ccgctacggg cgttgcactc accaatgggc gcatcatcaa ttcatcgacg gtggtggcga
     2881 cgatcggcaa cgggccgcat ccgctggtgc agacgcttgg cctcgacatg cactggggcc
     2941 gcatccggac agaccgcttc atgcaggtgc cagggcaaga acatgtctgg gcgctgggcg
     3001 atgcggcgct cattccgctc aacgacaacc cgggcgaaga cccggccgac tacgcgaccc
     3061 agacggcgca gtttgccgtg cgcgaaggat accagctcgc aggcaacgtc gtggccaagc
     3121 tgaagggtga ggagctcaag ccgttcgcct acaagtccaa gggctcgctc gcctccctcg
     3181 gcatgagcaa ggccgtggcc gatgtctacg gcataaagct gtcgggcatg ttcgcttggc
     3241 ttctgtggcg cggcttttat ctgtcgttcc tgccgggctt cgtcgccaag ttccgcgtgg
     3301 ggctcaactg gatgatcaac gccatcatgc cgccgaacat cgtgcaactt aggatgcggc
     3361 cgccggcgac ccgctacatc catttctgga ggggcgacaa agtgttcgag ccgggcatgc
     3421 tgatcgacgg cttctatacg gtgattaaag gctccttcaa actgaccatc gacaaccccg
     3481 acacggacga gcatttcgag aaagtgttcg gccctggcga acatttcggc gaacgcgtcc
     3541 tggtcaagtc ggagctgcgc accgggctgg tgcaggcact ggaggacggg ctgttgctgt
     3601 tcatcgccca gaaggatttc acccgcttcg cgacggcctt ccccacgctc gatgcctatt
     3661 tcaaggacta tattgaggag acctttggcg gaccggacaa ggcctttgcg ccgggcaata
     3721 tcgatcagtc ggagctggag aagactcact aggcgcgccg gcaccgaagc ggtggatgag
     3781 cgggcgaagc gattcccttg gagcccccgt aattgctata tttccgtcat catgacagca
     3841 gccaagacct cccaatccgc tgccggaaaa ccggccgggc gcggcgacca cgtgtacctc
     3901 atcgacggct cgggctacat cttccgggcc taccatgccc tcccgccgct cacgcgcccc
     3961 tcggacggcc tgcccgttgg cgcggtccat ggcttttgcg gcatgttgtg gaagctgttg
     4021 cgcgagaccg gcgagctcgc cccgccgacc catatggcgg tgatcttcga ttattcggcc
     4081 aagacgttcc gctccgacct gttcgacggc tataaggcca accgcccgga gccgcccgaa
     4141 gacctgatcc cgcaattccc gctggtgcgc gatgcggtgc gcgcgttcaa cgttgcctgc
     4201 atcgagaagg agggctttga ggccgacgac ctgatcgcca cctacgcgct gcaggccgtc
     4261 ggtgccggcg ccgacgttac catcgtctcc tcggacaagg atttgatgca gctcgtacgc
     4321 cccggcatcg tcatgtacga cacgatgaag aacaagaaga tcgccgagga aggcgtattg
     4381 gcgaagttcg gcgtgccgcc ttccaaggtc atcgaggtgc aggcgctgat gggcgactca
     4441 tccgataacg ttcccggcgt gccgggcatc ggcgtcaaga cagcggcgct tctcatcaac
     4501 gagtatggcg acctggaaac gctgcttgcc cgcgcctcgg agatcaaaca gaacaagcgc
     4561 cgcgagaacc ttatcgagtt cgccgagcag gcgcgcctgt cgaagaccct cgtgacgctc
     4621 gatacgcaaa cgccgctcga cgtgccgctc gcagagacgg ccgtgcagca gccggagccg
     4681 gagacgctta ccgccttcat gcgcgagatg gaattctcca cgctgttgaa gcgtgtcgcg
     4741 gaaggtttgg gcgcgccgtt gccgaagggc gccacgaaac caagtgccgt gcgaagaaag
     4801 aaggacgact acgaccatcc tttgcgcaag agcccgcgcg gggctgcggg ccccgcccgc
     4861 gacatcgacg tgcaaggcgg ctcgcccgcg gaactcgcca tcgaacgcgc aagcaagctc
     4921 gaagagattc ccttcgaccg caccaactac gagacggtga cggacatcgc gcgtttgcaa
     4981 gacttgctca agacagcgca gttccaagga aaccttgcga tccgcgtgaa gctgaacacg
     5041 tccgatccca tgacgggcga gatcgcgggc gtggcgctcg cgcttgcgcc cggccacgcg
     5101 gcctatgtgc ccttaagcca tcgcgcgggc gacgggctcg atctcggcgg cgaagacgtg
     5161 gggccgcaag cacccttgcg cgaagcgctc gacctgctga agccgatcct cgaagatgag
     5221 tccgtgctga agatcgtggc gggcgccaag cccgacatgg tggcgctcgc ccgctatggc
     5281 atcgcgcttc aggccgtgga cgatccctgc ctcatctctt atgcgcttga tgcgggccgc
     5341 gccgaccatg cgcccgcaaa gcttgccgcc gcgcggctcg gccataccgc gctcacggaa
     5401 aaagaagtgc tcggtaccgg caagtcggcg atcgcgtttg acctcgtcga ggtggcgcgc
     5461 gccaccgaag cccccgggga ggaagcggat atcgcctttc ggctgtggat gctcctgaag
     5521 ccgcgcttgg cggcggagca ggtgacgacg gtctacgaga cgctggagcg gccgctgcag
     5581 ccggtgctcg ccgccatgga gcgcgccggc atcaaggtgg agcggtcggc gctggcgaac
     5641 ctgtccggcg ctttcgcgca gaccatcgcg cgactggagg acgagatccg cgaccttgcg
     5701 ggcgaagatt tcaacatcgg ctcgcccaag caactcggcg agatcctgtt cgaaaagatg
     5761 agccttccgg gcgggcgcaa gacaaagacc ggcgcctgga gcacggacgc ggccgccctc
     5821 gaggaactgg ccgcagaagg ccacgacctg ccgcgcaagg tcttggaatg gcggctgctc
     5881 gccaagctga ggagcaccta tacggacgcc ttgcccgcct atattcacgc cgagacggga
     5941 cgcgtgcata cgcgctatgt gctggcctcc accacaacgg gccggctcgc cagccatgag
     6001 ccgaacctgc agaacattcc cgtgcgcact aaggaaggcc gctccatccg caaggccttc
     6061 gtcgccgaga agggcaagac gctgatctca gccgattaca gccagatcga gcttcgggtc
     6121 ctggctcaca tggcggacac gcccaccttg cgccaagcct ttgccgacgg gctcgacatc
     6181 cacgccatga cggcgagcga gatgttcggc gtgccgatcg agggcatgga ccccgctgtg
     6241 cggcgccgcg ccaaggcaat caatttcggc atcatctacg gcatctcggc ggtcgggctc
     6301 ggcgcacagc tcggcatccc gcgcggcgaa gcggccgcct acatcaacac ctatttcgag
     6361 cgcttccccg gcatcaagga ctacatggaa gcgatgaagg cggaggtgaa gcgctcaggg
     6421 ttcgtgacga cgctgttcgg gcgcaaagtg cattaccccg agatcaacac gaaaaaccca
     6481 agcttacgcg gcaactacga gcgcgcggcg atcaacgcgc cgatccaagg ctcagctgcc
     6541 gacatcatcc gccgcgctat gatccgcatg gacgaggcgc ttctcgatgc ggggctgtcg
     6601 gcgcgcatgc tcttacaggt ccacgatgag ctgatcttcg aggcgccgga ggacgaagcc
     6661 gagaagacta tggaggtcgc gcaggagatc atggaacacg cgcccgagcc tgcgctacgc
     6721 ttgcgggtgc cgctgaaagt ggacgcccgc gccgccgcta attgggaggc tgcgcattag
     6781 cgcttagccc gcgacaaagg agatttcatg cgtaccgcat ttgccctgct cgtttcgtgt
     6841 ctgatcgcgc tgcctgcgct cgcgctaccg gcgcccatgt cggaggcgga gcttgaggag
     6901 aagagcggca tcgtcgcgct ggtgcgtgtg atgtcggtga cgtgcaccag catcatcaag
     6961 gacaagacga ccggcgagga cctgcccggc tatatcgcca agctgcgtgt gatcaaggcg
     7021 aagaagggcg acgtcagacc gggcggcgaa gttcttgtca cgtggcgagc caagtcgcaa
     7081 aaactcatcg gaggttggac cgtcccctac tatccgggcg aggaggtgtg gacgcatctg
     7141 accaagcgcg cgggcggcgc aacctatgcc tcgacctggt ggaacgccaa gggcgacgag
     7201 gtgaagccgc cggagacgaa ggagctgccg acggtcgtcg gcaagtcggc gctttccgac
     7261 gaccaggact aagccgagag gttatccagc cacatgttga acagcgcgcg gctcgcctcg
     7321 ttggtgggtg gggcgtagcg cgcggtctcg gtgcgcattt gcgcgaggtc cacgttgttc
     7381 ttggccagct ccagcgtatg cccgatcagc cagcgctcca tgtcgcgccc cggtagctcc
     7441 acgtgaaatt gcagcgccag aactttcggc ccgtaggaga aggcttggtt cggcgtgagg
     7501 tccgtggagg caagccgcgt ggcgcccgta ggtatgtcga atgtgtcgcc gtgccaattg
     7561 agaatgtgca ggccttcgag cacgccaagg ggcgagtcct tgccttcgtc ggtcagggtc
     7621 agcgtgtccc agccgagctc ggcctgcttg cccggataga cgcgggcggc gagcacagcg
     7681 gcaagcgcct gggcgccgag gcagatgccg accacgggcg tgccctgctt gaccaatttc
     7741 tcgacgaggg caagctcttc tttgaggaaa ggatagcgtt cgatctcgta aatgccgatg
     7801 gggccgccaa gcacgagcac gaggtcggca tcgagtgccg gggaaagatc atcgcggccg
     7861 gcttgtaaat aggtgagctc gacgccgcgc tcctccagca caggaccgag cgtgcccgca
     7921 tcctcgaaag ccacgtgata gatggcgacg gcgttcttca tggcgtccct ctttgcttgg
     7981 cccgcatcat cttcgcgccc gaagttctcg ctagttgccg tttcgcggcg cgcggttgac
     8041 cttttgcgac acctataccg ttcagacggt ctgtgacacg ccttggcggc gcggatcgcc
     8101 ggcgccctcc acgccacggg tttcatgcca acgggcggca tgcacgccgc cgaagaacag
     8161 gttccgctcc ggccaggccc gtgccttgtc gccggcagcg cggaaggcgg cttctgtctc
     8221 ttccggaaag ccgggttcga agctcgtggt accgcagcgc tccacatgga gccggggcgc
     8281 gttcaccgca tcctccaggc tcatgccatg agccagcaga ttgatggcga cctgcaggat
     8341 cgccgtgcgg atgcggttgg agcctccggt gccgagcgcg gtcacggtac catccggctc
     8401 catgatgatt gtgggcgcca tcatcgaaga catgcgcgac ccttcgcgcc aggcatcgag
     8461 atcgccgaaa gctagatcct cctcgccgag catgttgttc agcatgaagc cgtgattgcc
     8521 gacgatgaag ccgtccccct cgccgttcga caggctgacc gcggcggcat tgccgtcccc
     8581 atcgatcacg ctgacatggg tcgtgccacg atagccgttt gggtattgcg cggccgcggc
     8641 aatctcgttc gcaagtgcgc cgtccgccag acgcgccgcc aggtcctcgc catggttggc
     8701 ccgtgccgcg ttggtcgcgg tcattgccgc ttgcagggcc tccgcgtcgc ttgcgccgcc
     8761 cgcttctgca tgcgcgccga gataagccaa gccgaacgct atgagcgcac cgcctgccga
     8821 gggcggcgga ttgagcgcga cggtcgcgcc gccgtggcgc cagagcagcg gatcgcgtaa
     8881 ggccacgcga tagcgcttga gatcgtcctc cgtcaggtgc ccgccaagct cgcgcgactg
     8941 cgcaacaata gcctggccga tatcgccatc gacaaagagc cgcgcaccgt cctcggccag
     9001 ccattccagc gtctcggcaa ggccgtcatt gcggaaggtt tcgcccgcct ccatgagctt
     9061 gccgtttgct gcgaagatcg cgcgggcacc ttcgcactct cgcaagattg gcgcgatgac
     9121 cgtgaagagg taggcctgaa aactgttcag cggaaaacct tggcgcgcgg cgcgggcggc
     9181 gggctccacc aagcgcttca tcggcaggcg cgcgtaagcc tcgtgcatgg cgaacaggcc
     9241 ttgcgcgata ccgggcgcgg ccgcggcccc aaggccgata tgaaactcct gggtggcggg
     9301 gccgaaatcg gcctcaatgg gaaagaacga aacctcgttc gccggccggc ggcgcagcgg
     9361 cgtctccacg aagaagtcga acagcttgat cgcgttgctg ccggcctggc gcgccatgag
     9421 aaacccgccg ccgccgggcg aagcgaatac ggcctcggcg acgaagctca tgcatacgcc
     9481 cgcgatcgcc gcatcgaagg cgttgccgcc atcggccaaa atctcggcgg cagcagaggc
     9541 ggtcaacgca tgaccggccg cgacgacact ctttgtcttc gtcatgggga tgtgcctaag
     9601 gcgagatgag gaaccgggca agcccgttgc gtcacgctac aaacgatcat ggccgggcgt
     9661 aagcccggcc atgagataag tctgaatgaa gcgcggtttg gactagccga ggcgcttctc
     9721 gagatcgtcg agctcttcct tgaggccgcc cggcagcttg tcgccaaagg tctcgtagtg
     9781 ctccttgatc atcggaagct cgtccttcca gcccttagtg tcgaccttga gcagttcctc
     9841 gaggtcggca gcgtcgacgt ccaggccgtc ggtgtcgagc gcatccttcg tcggcacctt
     9901 gccgatcggc gtgtcgaccg catcggtcgt gccgttgcaa cgctcgaaga tccacttgag
     9961 cacgcgggaa ttctccgcga agcccggcca caggaacttg ccttcggggc tcttccggaa
    10021 ccagttgacg tagaagatct tcggcatctt gtcgccgccc ttctcgccca tcttcagcca
    10081 gtgcgcgaag tagtcgccca tgtggtaacc gcagaagggc aacatggcca tgggatcgcg
    10141 gcggatgtcg cccttggcat ccgcggcagc ggccgtcttc tccgacgcca tgatggaacc
    10201 gaggaacgtc ccctgcttcc agtcctccgc ctcgacggcg agcggcacca cggaggcgcg
    10261 gcggccgccg aacagaatgg cgtcgatcgg cacgcctttg ggatcctccc attccggcgc
    10321 gatgaccggg cactggccgg ccggtgcggt gaagcgcgcg ttgggatgcg ccgcggggcg
    10381 gcctgattcc ggggtccaag agcgacgcag ccagtcgatc gtgtgcgccg gagactcctt
    10441 tgtcatgcct tcccaccaca cgtcgccgtc atcagtaagt gcgacgttgg aatagatgca
    10501 gttcttgtcg agcgctttca tagcgttgag gttcgatgcc tcggacgttc ccggcgccac
    10561 accgaagaag ccggtctccg ggttgatggc gtagaggcgc ccgtcctcac cgaacttcat
    10621 ccagcagatg tcgtcaccga tgcactcggc cttccaacct gggatcgtcg ggtcgagcat
    10681 ggcgaggttg gtcttgccgc aggcggaggg aaaagccgct gccatgtact tcacgtcgcc
    10741 ttccgggttg gtgagcttga ggatgagcat gtgctcggcc atccagccct cgtcacgcgc
    10801 cttcacactc gcgatgcgca gcgcgtgaca cttcttgccg agcagcgcgt taccgccata
    10861 gcccgaaccg taggaccaaa tctcgcgcgt ctccgggaag tgcgagatgt acttggtctc
    10921 attgcacggc cacggcacgt cggcctgctt gtccgaggtc agcggcgcgc caaccgtgtg
    10981 cctgccgcac acgaagtctg cggagtcgcc gagcgccttc cataccgcat cgccgacgcg
    11041 ggtcatgatg tgcatgttgg cgacggcgta ggcgctgtcg gtgaccatca cgccgatgcc
    11101 cgcgatgggt gagccgacgg ggcccatgga gtacggcatg acatacatgg tgcggccctg
    11161 catggcgccg tcgtagagct tcgccatcgt ctccttcatc ttcgccgggt cgaaccagtt
    11221 gttggtcggg ccggcgtccg cctcgctctc ggagcagatg taggtctggt cctcgacgcg
    11281 ggcgacgtct ttggggtcgg agcgaacgag aatcgagttg ggacgcttct cctcgttgag
    11341 ccacatggca gtgccgccgt cgaccagcgc cttcagcatc gcctgatact cttcctggga
    11401 gccgtcacac caataaagag agtccggctt gaagagctcc tttgcctctt ccacgaaagc
    11461 caagaccttc ttgttcgtgg tgggcgcaat tttcgactga acgttcatac ggtttcgtcc
    11521 ctaaatctcg aatggatgga aaaaatcgct atggatcgcc ctctagctac cctgaacttt
    11581 gagggctttc aagaaaggtc ctgtgagaga ggcgacttcc gatggaaccc gcaaggcttc
    11641 aagaggaatt gaccgcgctt ctggcttctt cggccaggcg gaccagctct tttcgggcat
    11701 ctgccggggt cagcacggac ttttcgaacg aaatccgggc cccatcaacc ccaagcgcga
    11761 tatcgcagcc ttcggggtcg ataccgatca tacgccaggg gccaggatgg cggccggcga
    11821 aggctcgggc atagagctcg atggcgtcgg cgtggtcatc gttcatgtgg gcaacgatgt
    11881 ccggctcggc cgcgaggagg gtttccgccc ctttttgcgg caacagcagg tcgccagggc
    11941 tcagatccac gatgcgacca aatccgccga tatagtgcgc accctccaag tccaggcgcc
    12001 aaaaggaaaa atcggggaaa tcggcataga acgccgcctc tggatgccgg accaagaagc
    12061 ggcgccgcac cacctcgtcg tcgacccgtc cggccttccc agacagggtc agtcgggcgc
    12121 cttggagagg gtcggccgcc gcccccgagc catcgatcag gatcgcggcg cgggggtc
//
LOCUS       DBNHVU010000120         8045 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_47142, whole genome shotgun sequence.
ACCESSION   DBNHVU010000120 DBNHVU010000000
VERSION     DBNHVU010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8045)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8045)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8045
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_47142"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1374)
                     /gene="fabF"
                     /locus_tag="AC16Y7_04765"
     CDS             complement(109..1374)
                     /gene="fabF"
                     /locus_tag="AC16Y7_04765"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR5708726.1"
                     /translation="MRRVVITGLGIVSPLGSGHETAWRRLTAGENAAQRIDEFDVSDL
                     SCQIGCQVPRGDGSDGSFNPNDWMEHKEQRKVDDFIIYAMGAATQALTDAGWKPTAYE
                     DQIRTGVLIGSGIGGLQGIEKCSLLFADKGPRRVSPFFIPGRLINLASGYVSIEHGLK
                     GPNHAVVTACSTGAHAIGDASRLVALGDADVMLAGGSESPICRLALAGFAACRALSTN
                     FNDQPQKASRPYDRDRDGFVMGEGAGMMVIEELEHAKARGANIYAEIIGYGLTGDAFH
                     ITAPAEDGDGAFRCMSAAVKRAGIDPADIDYINAHGTSTPMGDEIELGAVERLFGNAA
                     GKVSMSSTKSATGHLLGAAGAVEAIFSVLAIRDNVAPPTLNLDNPSVATAIDLVPHKA
                     RQRNIDTVLCNSFGFGGTNASLVMRRYAA"
     gene            complement(1473..1712)
                     /locus_tag="AC16Y7_04770"
     CDS             complement(1473..1712)
                     /locus_tag="AC16Y7_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR5708727.1"
                     /translation="MSDISERVKKIVVEHLGVEADKVADNASFIDDLGADSLDTVELV
                     MAFEEEFGCEIPDDAAETILTVGDAIKFLEKNTAA"
     gene            complement(1953..2690)
                     /gene="fabG"
                     /locus_tag="AC16Y7_04775"
     CDS             complement(1953..2690)
                     /gene="fabG"
                     /locus_tag="AC16Y7_04775"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIR5708728.1"
                     /translation="MFDLSGKNALVTGATGGIGGAIAKALHGQGASVAISGTNAGKLE
                     ALATELGERVFVLPCDLRDRPAVVQLAETAEKTLGQVDILVNNAGITHDNLFMRMKDE
                     EWDDVIAVNLTSVFTLTRGILRGMMRRRAGRIVNIASISGVFGNPGQGNYAASKAGLV
                     GMTKSLAREVAARGITANCISPGFITTPMTDALTEKQQEAIAAAIPAQSFGKPDDVAA
                     ATVFFASDESAYVTGETMHVNGGMIMI"
     gene            complement(2709..3662)
                     /gene="fabD"
                     /locus_tag="AC16Y7_04780"
     CDS             complement(2709..3662)
                     /gene="fabD"
                     /locus_tag="AC16Y7_04780"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR5708729.1"
                     /translation="MSIALIFPGQGSQAVGMGAELAKAYPAAQAVFSEVDEALGQKLS
                     HLMWEGPEADLTLTENAQPALMAVSMAAMRVLSDTKGVSLAETAAYVAGHSLGEYSAL
                     AAAGALSLADTARLLKTRGRAMQAAVPVGEGAMAALLGADLEQAQELAKAAAESDVCQ
                     AANDNAPGQVVISGSRAAIERAVELAPKYGARRAVLLPVSAPFHCALMQPAADAMAEA
                     LAGVEINAPAVPVIANVLAEPISDPEAIRSRLVEQVTGMVLWRETMAFLASKDVNAVY
                     EVGAGRVLTGIARRFDGIEAKSLGTPDELEAASVALQGGSA"
     gene            3974..4453
                     /gene="rpsF"
                     /locus_tag="AC16Y7_04785"
     CDS             3974..4453
                     /gene="rpsF"
                     /locus_tag="AC16Y7_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR5708730.1"
                     /translation="MPLYEHVFLARQDISAQQVEGLVDTYRSLLEENSGKVGKTEYWG
                     LKSLSYRIKKNRKAHYTLINIEAPHEAVAEMERQMGLSTDVIRFMTIKVDEHETEPSA
                     MMRRSDDRDGRGDRGRHHGGRRDHGPREPRPERTEAAKPSEPAAEEKKEETKEESGE"
     gene            4450..4695
                     /gene="rpsR"
                     /locus_tag="AC16Y7_04790"
     CDS             4450..4695
                     /gene="rpsR"
                     /locus_tag="AC16Y7_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR5708731.1"
                     /translation="MSSTSMQARRPFYRRRKTCPFTGDKAPTIDYKDVKLLQRYVSER
                     GKMVPSRITAVSAKKQRELARAIKRARFLGLLPYLIQ"
     gene            4796..5776
                     /locus_tag="AC16Y7_04795"
     CDS             4796..5776
                     /locus_tag="AC16Y7_04795"
                     /inference="COORDINATES: protein motif:HMM:NF021506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HIR5708732.1"
                     /translation="MPTQILIGVGAGLVSAALFASAALAGAFAGIPFYLAPLPLCLAG
                     LGWGRATVLIAAATGALLLAVVLSAPLGLAFGLTLGLPIVTLIHLLLLARPAAVPNEE
                     GAAALEWYPPGRLVGWAAMMAGFLAGLLALALGPDVETYRQQVGALLSPELIAALDPD
                     GNIFTPETVEKLKVVLAKTLPAAFAVVWLIIVLFNLWVGGFIVESSGRALRPWPDLHA
                     LEVPNAFVAVFAVALALSFLPGIIGVLATGFAGAMLFAYVLQGLAVIHTYSLGMTMRP
                     LLLVAVYLGILFLGWVAIVVAIIGLGEPLFGLRRGPRTPNTPPSRDDETD"
     gene            5798..6433
                     /gene="rplI"
                     /locus_tag="AC16Y7_04800"
     CDS             5798..6433
                     /gene="rplI"
                     /locus_tag="AC16Y7_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012754955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR5708733.1"
                     /translation="MEVILLERIGRLGQMGDVVNVKDGYARNFLLPRGKALRATDTNR
                     KHFESQRAQLEARDLEAKTEAEAVSGKLNGETFIVIRQAGDNGQLYGSVSTRDISTAV
                     TEGGFSIERRQVLLDRPIKALGLHEVRVSLHGEVEPNIVVNVARSEEEAARQARGEDV
                     TRTLSDEEEDAEAALEAAESLFEEGAKPEEEQPSEAVEETTEKTSEDETPA"
     gene            6575..7831
                     /locus_tag="AC16Y7_04805"
     CDS             6575..7831
                     /locus_tag="AC16Y7_04805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5708734.1"
                     /translation="MFRPLVNTLERTVTTGHLRLVDSDGCAYDFGDRSDPQVIARITD
                     KRTERQLTLNAPFAIGEAYMDGRLVLEHGTIYDFLELLLTNAQHRQPRWLHFLDRFMY
                     ATQRLQHFNPIGRSKRNVAHHYDLDSSLYALFLDPDRQYSCAYFEHDGMSLEDAQEAK
                     KRHLAAKLYLADGMHILDIGSGWGGLALYLAKTAHVEVTGITLSEEQLRIARERARAE
                     GLAKAVNFELCDYRNLEGKFDRIVSVGMFEHVGSAHYRMFFRQMNRLLTDNGVAVLHS
                     IGRFDGPAATNPFIAKYIFPGGYIPALSEVLPAVEREGLLATDVEILRLHYAMTLRHW
                     RQRFRGAWHTAAKKFGERFCRMWEMYLAGSETAFRYQNLMVFQLQVTKDQSALPLTRD
                     YFFDAERDLRARDRHATPSRKRRSSR"
BASE COUNT         1516 a         2670 c         2360 g         1499 t
ORIGIN      
        1 ctcgaagaag tcatgctgga ccagcgcgcg cccccgcgga tgtctaagcc actttgcgtc
       61 caaatatggc gaaagggagg ttcgcctgac aagcaaaagc gtaacagatc aagcggcgta
      121 acggcgcatg actagcgatg cgttcgtccc gccaaatcca aaggagttgc acaacaccgt
      181 gtcgatgttc cgttgacgcg ctttatgcgg cacgagatcg atggccgtcg caaccgaagg
      241 attgtccaga ttgagcgtag gcggcgccac gttgtcgcgt atcgccaaca cggagaagat
      301 cgcttccacg gcgccggcgg cacccaggag atgtcccgtc gccgacttcg tcgacgacat
      361 ggagaccttg ccggcggcat tgccgaaaag ccgctccacg gccccaagct cgatctcgtc
      421 acccatgggt gtcgaggtgc cgtgtgcgtt aatgtagtcg atatcggcgg ggtcgatgcc
      481 cgcccgtttc acggccgccg acatgcatcg gaaggcgcca tcgccatcct cagccggcgc
      541 ggtgatgtgg aacgcatcgc ccgtcaagcc gtagccgatg atctcggcgt agatattcgc
      601 gccacgcgcc ttggcgtgct cgagttcctc gatcaccatc atgccggccc cctcgcccat
      661 gacgaacccg tcgcggtcac ggtcgtaagg ccgggaggcc ttctgcggct gatcgttgaa
      721 gttcgtcgac agcgcgcggc aggctgcaaa accagcaagc gccagacggc agatcggcga
      781 ttccgaaccg cccgcaagca tgacatccgc atcgccaagc gccaccagcc gcgacgcatc
      841 gccgatggca tgggcacccg tcgaacaggc ggtgaccact gcgtggttag ggcctttcag
      901 cccatgctcg atggagacgt agcccgaagc gagattgata aggcggcccg gaataaagaa
      961 ggggctgacc cggcgcgggc ccttatcggc aaacagcagc gagcacttct caatgccctg
     1021 caggccccca atgccggacc ctatcaacac gcccgtgcgg atctgatcct cataggcggt
     1081 cggcttccag cccgcgtcag tcagtgcctg cgtggccgcg cccatcgcgt agataataaa
     1141 gtcgtcgacc ttgcgctgct ctttgtgttc catccagtcg tttggattaa acgagccgtc
     1201 gctgccgtcg ccccgcggaa cctggcagcc gatctggcag gacaaatccg agacatcgaa
     1261 ctcatcgata cgctgggccg cattctcacc cgccgtcagc cgacgccaag cagtctcgtg
     1321 accgctgccg agcggcgaga caatacccaa acctgtgatg acgacacgtc gcatcgcgcc
     1381 ttcgcgtgat ctcaagaaag accggttatc gagcccaacg ctcgctcgtc ttaacgcgcg
     1441 gacggcaaaa tgcgttcgcg cgaggccggg gtttacgccg ccgtgttctt ttccaggaac
     1501 ttgatggcgt cgccgacggt gagaatcgtc tcagcggcat cgtccgggat ctcgcacccg
     1561 aactcttcct caaatgccat caccagctcg accgtgtcga ggctgtccgc cccgaggtcg
     1621 tcgatgaagc ttgcattgtc ggcgaccttg tcggcctcaa caccaagatg ctccaccaca
     1681 atctttttta cgcgctcaga gatatcgctc atgtcctcgt caccgtccct taactatgtc
     1741 ctcgcgtacc cccgagcccg cggctcatgg ccttgtaccc ccgagcggca attcaggggc
     1801 gttgatcgcg gcgggattgt cctttgtcaa ccccccaccg aacggccgtt tgggtaacac
     1861 actctatatg actttgccag aagccctcgt gcggcacggt gccttgccaa tcgttctcag
     1921 cgtgaccgcc taacccattg ttacaacggt cattaaatca tgatcatgcc gccgttcacg
     1981 tgcatggtct cgcctgtgac ataggcggac tcgtcgctgg caaagaaaac cgtcgccgcg
     2041 gcgacatcgt ccggtttgcc gaagctctgc gccggaatcg cggcagcaat cgcctcctgt
     2101 tgtttctccg tcagcgcatc ggtcatcggg gtcgtaatga atccgggtga gatgcagttc
     2161 gccgtgatgc cgcgcgcggc cacttcgcgg gcaagagact tggtcatacc aacgagcccg
     2221 gctttcgagg cggcgtaatt gccctgcccg gggttaccga agacgcctga aatcgacgcg
     2281 atgttgacga tacggcctgc acggcggcgc atcatgcccc gcaatatccc gcgggtgagc
     2341 gtgaacacgg acgtcaagtt gacggcgatg acgtcgtccc actcctcgtc cttcatccgc
     2401 ataaagagat tgtcgtgggt gatgcccgca ttgttgacga gaatatcgac ctggcccagc
     2461 gtcttctctg cagtctccgc aagttggacc acggctggcc ggtcgcgcaa atcgcaaggc
     2521 agcacgaaca cgcgctcgcc gagctctgtg gcaagggctt cgagtttgcc cgcgttggtg
     2581 cccgaaatcg ccaccgaggc gccctgccca tgcagcgcct tggcaatggc cccgccgatg
     2641 ccgccggtgg cgccggtcac caacgcattc tttccgctga gatcgaacat ttagaagctc
     2701 tcccttcctc aggcgctgcc gccctgaagc gccacgcttg cggcctccag ttcgtcgggc
     2761 gtgcccaaac ttttggcctc gatgccatcg aaacgtcttg caatgccagt tagcacacga
     2821 ccggcgccca cttcgtagac cgcattcaca tccttgctgg caagaaaggc catggtctcg
     2881 cgccagagca ccatacccgt gacctgttcg acgagccggg accgaatggc ctccggatcg
     2941 gagatgggtt cggccagcac attggcaatc accggaaccg ccggcgcatt gatctccaca
     3001 ccggcgagcg cttcggccat cgcatcggcc gccggctgca tcagcgcgca atggaacggc
     3061 gcgctgacag gtaggagcac cgcacggcgg gcgccatatt tcggcgccag ttcgacggcg
     3121 cgctcgatgg cggcccgact gccggagata accacttggc ctggggcgtt gtcgttcgcg
     3181 gcctggcaca cgtcactctc ggcggctgcc ttggcgagct cttgggcttg ctccaggtcg
     3241 gcgccgagaa gcgcggccat ggccccttcg cccacgggca ccgccgcttg catcgcccgc
     3301 ccacgggtct tcaggagccg cgccgtgtcg gcaaggctga gcgcgccggc tgcggcaagc
     3361 gcggaatatt cgccaaggga gtgcccagcc acataggcgg ccgtttcagc gagcgacacg
     3421 cctttggtat cgctcaagac ccgcatcgcc gccatggaga ccgccatcag ggccggctga
     3481 gcgttctcgg tcagcgtcaa atcggcttcc gggccttccc acatcaaatg tgagagtttc
     3541 tggccaaggg cctcatcaac ctcagaaaaa acggcctggg ccgcaggata tgccttggcc
     3601 agctcggcac ccatgccgac ggcttggctt ccttggccag gaaaaatcaa agcaatgctc
     3661 atgtgctgaa aatcgtggcg gttgacagag gaccgccaaa gacactgttt caatctgacc
     3721 tgtcaagcaa gtggatgccg cgggggcccc gacggccttg caaagcacgt ggcaggctct
     3781 ataaagcgcc caccagcaac cgctccggtc ggaggctgaa cggaggaccg ctttcgcggt
     3841 aggcttgaac ggccgccctc ccgtgtctcc gctcttctgg gcatgcgctc taaagagctt
     3901 tcttgggcct ttccttacca atgcgcccag gttgaggctt cgcgccggag caatgaagaa
     3961 ggaaaggcta ttcatgcctc tctatgagca tgtgttcttg gcgcgccagg atatctcggc
     4021 gcagcaagtc gaaggtctgg tcgacaccta ccgctctctc cttgaggaga atagcggcaa
     4081 ggtcggcaag actgaatatt ggggcctcaa gtccctctct taccgcatca agaagaatcg
     4141 caaggcccac tacacgctga tcaacatcga ggcaccccac gaggcggttg cggagatgga
     4201 gcggcaaatg ggtctgtcga ccgatgtcat tcgcttcatg accatcaagg tcgatgagca
     4261 cgaaaccgag ccttcggcca tgatgcgccg ttcggacgac cgcgacggtc gtggcgatcg
     4321 aggccgtcac catggcggac gccgtgacca cggcccgcgc gagcctcgtc ccgaacgcac
     4381 cgaagcggcc aagccaagcg agcccgctgc tgaagagaag aaagaagaga cgaaagagga
     4441 gtccggcgaa tgagctccac ctccatgcag gcccgccgcc cgttctaccg ccggcgcaag
     4501 acctgcccct tcacaggcga taaggcaccc acgatcgact acaaggatgt gaagctgctc
     4561 cagcgttacg tgtccgagcg cggcaagatg gtgccaagcc ggatcacggc ggtgtcggcc
     4621 aagaagcagc gcgagctggc ccgggccatc aagcgcgccc gtttccttgg gctgttgccg
     4681 tatctcattc agtaggctga cgcgccaaac acggcgcacg acgtgacgag aaagttgggg
     4741 taggcctgat ggcctcccct aaccgctctt caaaggaggc gggacagcga aaacgatgcc
     4801 aacacagata cttattggcg taggcgcagg ccttgtctca gcggctctct ttgcatcggc
     4861 cgctctggcg ggcgcattcg ccggcattcc cttctatctc gcgccgctgc cgctgtgcct
     4921 cgccgggctc ggttggggcc gcgccactgt cctgatcgcg gccgcgacag gagcactgct
     4981 cctagccgtc gtactcagcg cccctctcgg gctggctttc gggctcacgc tgggcctccc
     5041 catcgtcacg ctcatccacc tcctgcttct ggcgcgcccc gccgcagtgc cgaatgagga
     5101 aggcgctgcc gcccttgagt ggtatccccc tggccggctc gtcggctggg cggcgatgat
     5161 ggccggattc ttggccggcc ttctcgccct tgcgcttggg ccggacgtgg agacctatcg
     5221 ccagcaggtt ggcgccctcc tcagtcccga actcatcgcg gcgctcgatc ccgacggcaa
     5281 catcttcacg ccggagaccg tcgaaaaact caaagtcgtt ttggccaaga ccctgccggc
     5341 cgcgttcgcg gtcgtctggc tgatcattgt cctcttcaat ctttgggtgg gtggattcat
     5401 tgtcgagtca tcgggccgcg ccttacgtcc ctggcccgat ctgcatgctc tcgaagtgcc
     5461 gaacgccttt gtcgccgtct ttgccgtcgc gctcgccctg tcgttcttgc cagggatcat
     5521 tggcgtgctc gccaccggct ttgccggtgc gatgctgttc gcctacgtgc tgcaaggctt
     5581 ggcggtgatt cacacctaca gcctcggcat gaccatgcgc ccgcttctgc tggtcgcggt
     5641 ttatctcgga attctgttct taggctgggt cgccatcgtg gtcgccatta tcggtctcgg
     5701 cgagcccttg ttcggcttac gccgcggccc ccgcacaccc aacaccccgc catccagaga
     5761 cgacgaaaca gactgacaac acataggagt agaacgcatg gaagtcattt tacttgagcg
     5821 catcggccgc cttggccaga tgggcgacgt ggtcaacgtg aaggacggtt acgcgcgcaa
     5881 ctttctcttg ccgcggggca aggcacttcg cgccaccgat accaaccgca agcacttcga
     5941 gagccagcgc gcgcagctcg aggctcgcga tctcgaagcc aaaacggaag ccgaggccgt
     6001 ctccggcaag ctcaatggcg agaccttcat tgtcatccgg caagccggcg acaacggtca
     6061 gctttacggc tcagtctcca cacgtgacat ctcaaccgcc gtcaccgaag gcggcttctc
     6121 catcgaacgc cgtcaggtcc ttctcgaccg gccgatcaag gcccttggcc tgcacgaagt
     6181 tcgtgtctcg cttcacggcg aggtggagcc gaatatcgtc gtcaacgtgg cgcggtccga
     6241 ggaagaggct gcccgccagg cgcgcggtga ggacgtcacc cgcactctga gcgacgagga
     6301 agaggacgcg gaagccgcgc tcgaagccgc cgaaagcttg ttcgaggaag gtgcgaagcc
     6361 cgaagaagag cagccgtcgg aggcggtcga agagaccacc gagaagacaa gcgaggacga
     6421 gacgcccgca tagtcacgcg ggggcgtgca ccgctgaagc cttaagtaac gctagaggca
     6481 ggctgttgtc ctgcctgcct ctagtctaag ctagccttgg ctcgacttcg acgactcggt
     6541 ggagcccgag gtcatgacta ggcgtgcgac atggatgttt cgcccgctcg tcaataccct
     6601 tgagcggacc gtcacaaccg gtcatctgag actggttgac agtgatggtt gtgcttacga
     6661 cttcggcgat cgcagtgacc cgcaagtcat cgcccgcatc accgacaagc gtaccgagcg
     6721 ccaactcaca ctcaatgcac cgttcgccat tggcgaggcc tacatggacg gccgcctcgt
     6781 tctcgaacac ggcacgatct acgactttct cgaactgctg ctgaccaacg cccagcaccg
     6841 ccagcctcgc tggctgcatt ttctcgatcg cttcatgtac gcgacgcagc gcctacagca
     6901 tttcaatccc attggccgtt cgaagcggaa cgtcgcccac cattacgacc tcgacagctc
     6961 cctctacgcg ctgttcctcg atccggaccg gcagtattcc tgcgcctatt tcgagcatga
     7021 cggcatgtcg ctcgaagacg cgcaggaagc caagaagcgc cacctcgcgg ccaagctcta
     7081 tctcgccgac ggcatgcaca tcctcgacat tggctctggc tggggcggcc tcgcgctcta
     7141 cctggccaag accgcccatg tggaggtcac cggcatcact ttgagcgagg agcagctcag
     7201 aatcgcgcga gaacgcgccc gagccgaagg cctggcgaaa gccgtcaact tcgagctctg
     7261 cgactaccgc aatctggagg gcaagttcga ccggatcgtc tcggtcggca tgttcgaaca
     7321 tgtcggatct gcccattacc gaatgttctt ccgccagatg aaccggctgc tcaccgacaa
     7381 cggcgtggca gtgcttcatt cgatcggccg cttcgacggc ccggccgcga ccaatccctt
     7441 catcgccaag tacatcttcc ccggcggcta catcccggcc ctcagcgagg tactgcccgc
     7501 ggtcgaacgc gagggcctgc tggccaccga cgttgagatc ctgcggctgc actacgccat
     7561 gaccttgcgt cattggcgcc agcgcttccg cggcgcctgg catacggcgg cgaagaagtt
     7621 cggagagcgc ttctgccgca tgtgggaaat gtacctggcg ggctctgaga ctgccttccg
     7681 ctaccagaac ctgatggttt tccagctcca ggtgacgaag gaccagtccg cgctgccgct
     7741 aacccgcgac tatttcttcg acgccgagcg cgacttgcgc gcgcgcgatc gccatgccac
     7801 cccgtcacgc aagcgccgga gcagccgcta gcgcctatca cacggtcatg cccccccctt
     7861 tgcgtggcgg ctgtgcacag cggtaatgaa actgtcacgt gggctgttga cctgttttcc
     7921 aggactacga atcacctagt cttctggcga tgagcaacgc ccttaccgca ccccgcttgg
     7981 cgcccgaaac ccccgttttc cgggagccac cccacaacct ggaggccgag caggccctgc
     8041 tgggt
//
LOCUS       DBNHVU010000121         7122 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_492168, whole genome shotgun sequence.
ACCESSION   DBNHVU010000121 DBNHVU010000000
VERSION     DBNHVU010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7122)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_492168"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /locus_tag="AC16Y7_04810"
     CDS             complement(<1..1368)
                     /locus_tag="AC16Y7_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR5708735.1"
                     /translation="MKRSYLDYAMSVIVSRALPDVRDGLKPVHRRILYAMRENNYTPD
                     RPYNKSARVTGETMGKYHPHGNQAIYDALVRLAQDFSMRLPLIDGQGNFGSVDGDPPA
                     AERYTEVRLAKPAMALLDDLENDTVDFQPNYDGREQEPVVLPAKFPNLLVNGAGGIAV
                     GMATNIPPHNLGEVIDACTAYLDNPEIEIAELNEIVPGPDFPTGGLILGRQGIRSAYH
                     KGRGSVTMRGRVAVEEIRKDRDALIITEIPYQVNKAAMIEKIAELVRDKKIEGIADIR
                     DESDRQGMRVVIELKRDAVADVVRNQVFRFSPLQSTFGCNMVALNGGRPELLNLKDMI
                     RAFTEFREEVVGRRIKFLLRKARDRAHVLVGLAIAVANIDEVIALIRKAPDPSTAREQ
                     LMGRDWPAKDVVALVELIADPRHTVSAKGTYHLSEEQARAILDLRLQRLTALGRDEIG
                     DELKGL"
     gene            complement(1783..2262)
                     /locus_tag="AC16Y7_04815"
     CDS             complement(1783..2262)
                     /locus_tag="AC16Y7_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR5708736.1"
                     /translation="MAGSINKVILIGNLGADPEVRHTQDGRPIVNLRVATSDSWRDKN
                     TGERREKTEWHRVVIFSEGLAKIAEQYLKKGSKVYLEGALQTRKWEDQSGQERYTTEV
                     VLQGFNSTLTMLDGRRDGGGMGESAGGGGGDFGRTKPLSSSGGGGSFNKELDDEIPF"
     gene            2580..3194
                     /locus_tag="AC16Y7_04820"
     CDS             2580..3194
                     /locus_tag="AC16Y7_04820"
                     /inference="COORDINATES: protein motif:HMM:NF013304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HIR5708737.1"
                     /translation="MQRSVAKRRSPVRRKKTKRRDPRTKSFGFTARQWPFIATLVGNW
                     MFAAAFFALAKTFWHWTPEAWGIADRLALVIKDAVIAIIPGVIAICIVAAQRLDPSMF
                     VGQVPKPNSPVDINTKFILNTFEQFTAWFIAMAGLAMYCPIEEARTLPILTVLFVLGR
                     ILFWVGYHTNPYLRAFGFGITFYPTVVAYAWLVLIMVFGIRVPL"
     gene            complement(3198..3758)
                     /locus_tag="AC16Y7_04825"
                     /pseudo
     CDS             complement(3198..3758)
                     /locus_tag="AC16Y7_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020010832.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2585 family protein"
     gene            3858..5426
                     /locus_tag="AC16Y7_04830"
     CDS             3858..5426
                     /locus_tag="AC16Y7_04830"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIR5708738.1"
                     /translation="MTQDEALALLKTGASIFLTGQPGSGKTHTVNRYLRDLAQAGVDV
                     AYTASTGIAATHGHGVTIHAWSGIGVREALTRRDLDVLASNRRLAARIEKTSVLIIDE
                     VSMLPPRALTLADTVCRHIRATNAPFGGMQVVLVGDFFQLPPVIRRRFDSSALPLAEE
                     DADSSSAFAHASAAWRDLDPMVCYLSEQHRQDDQPFLDILDAIRSNACTGIHRERLAA
                     RMVDRDQLPPSRTRLFTHNAAVDEINQQQLAKLDGETHTFAMTAKGAKPSIDALKRGC
                     LSPDEFAVKKNAVVMFTKNDPAGRYVNGTLGTVEDFDSETGSPIVRTRSGKRIFAEPV
                     TWKIEENGKERASVTQIPLRLAWAITVHKSQGMSLDAAVIDLSRAFEYGQGYVALSRL
                     RSLSGLHLLGLNERALLVHPAAIDTDAEFRRASHDALQVLERLGPDELAQRHAAFLED
                     CGASGTEAAPKSYDVASLRKAHGKAYAPWTETEEQDLRRRHQAGESVGAIAERLGRKP
                     GAIRSRLKKLALIP"
     gene            5518..>7122
                     /gene="uvrA"
                     /locus_tag="AC16Y7_04835"
     CDS             5518..>7122
                     /gene="uvrA"
                     /locus_tag="AC16Y7_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR5708739.1"
                     /translation="MTSKFISIRGAREHNLKTVSLDLPREKLVVITGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLDMMQKPDVDHIEGLSPAISIEQKTTSKNPRSTVGT
                     VTEIYDYMRLLWARVGIPYSPATGLPIESQTVSQMVDRVLELPEGTRLYLLAPIARGR
                     KGEYKKELAELQKKGFQRVKIDNEFYEIAEAPALNKKLKHDIDVVVDRVVVRNDVTAR
                     LADSLETALSISDGIAAAEFADEPDKRLIFSAKFACPVSGFTIEEIEPRLFSFNNPYG
                     ACPECDGLGTELFFEPELVVPDEALSLNRGAIAPWTRTSATSPYYQQTLESLAKHYKQ
                     SMTKPWKDLSEDFREIVLYGSLDDVNFVYDDGVRHYSTEKPCEGVITNIERRWRETES
                     NWVREELERYQSDQPCEACTGYRLKPQALAVKVAGLHIGEVPDMSIREAGAWFEALPE
                     HLTSKQNEIAARILKEIRERLTFLNDVGLEYLTLSRASGTLSGGESQRIRLASQIGSG
                     LTGVLYVLDEPSIGLHQRDNARLLETL"
BASE COUNT         1328 a         2293 c         2148 g         1353 t
ORIGIN      
        1 gagccccttc agttcgtcgc cgatctcatc gcggccaagc gccgtcaggc gttgcaggcg
       61 caggtcgaga atggcgcgag cctgttcctc cgataggtga taggtccctt tcgcggagac
      121 cgtgtgccgg ggatcggcaa tcaactcgac cagcgccaca acgtctttcg ccggccaatc
      181 gcgccccatc aattgctcgc gcgccgtcga gggatccggt gcttttcgaa tcagcgcgat
      241 cacctcgtcg atattggcaa ccgcgatagc aagcccgacc agtacgtgcg cacggtcacg
      301 cgctttgcgc agcaggaact tgatgcggcg tccgacgacc tcctcgcgga actcggtgaa
      361 agcgcggatc atgtccttca gattgagcag ctcggggcgg ccgccgttca gcgccaccat
      421 gttgcagccg aacgtcgatt gcagcggcga gaaccggaag acctggttcc gcacaacgtc
      481 cgcaacagcg tcgcgcttca gctcgatcac cacgcgcatg ccttgccggt cggactcgtc
      541 gcgaatgtcg gcgatgcctt cgatcttctt gtcgcgcaca agctcggcaa tcttctcgat
      601 catcgccgcc ttgttcactt gatacggaat ctcggtaatg atcagcgcgt cgcggtcttt
      661 gcgaatttcc tcgaccgcaa cgcggccccg catggtcacc gacccacgac ccttgtgata
      721 ggcggagcgg atgccctgac ggccgagaat caatccgccg gtcggaaagt cggggcctgg
      781 cacaatctcg ttcagctcgg cgatttcgat ctccgggttg tcgagatagg cggtacaggc
      841 gtcgatcacc tcgccaagat tgtgcggcgg tatgtttgtg gccatgccga ccgcaatacc
      901 gccggcgccg ttgacgagca ggttcgggaa cttcgccggc aacacgacgg gctcttgctc
      961 ccggccgtcg tagttcggct ggaagtcgac cgtgtcgttt tccagatcgt cgagcagcgc
     1021 catggccggt tttgccaggc gcacttcggt gtagcgttcc gccgcgggcg ggtcgccgtc
     1081 gacggagccg aagttcccct gcccgtcgat gagtggcagg cgcatggaga agtcctgcgc
     1141 aagccgcacg agcgcgtcgt aaatcgcctg gtttccatgg gggtgatatt tacccatggt
     1201 ctcgccggtg acgcgcgccg atttgttgta cggccggtcc ggcgtgtagt tgttctcgcg
     1261 catcgcatag aggatgcgcc gatgcaccgg cttgaggccg tcgcgcacat caggcagcgc
     1321 acgggacacg atcacgctca tcgcgtaatc gagataagac cgcttcatct catcgatgag
     1381 cgatacgggt cttatgtcag agggagttgg gccctcgacg ccggtgtctt cttcgtcagc
     1441 caaggaagga aagaagcctt ctcagaaagt gtgggttcct gaccaacgcc gcctgccgcg
     1501 agaggaaatg agaaccttcg atcgcgagtc ggatgcgctt tcaggagtag ttttctatag
     1561 ctgatttagg cgtcttaccg caacatcgaa tgcgcccgct acgcggatga ttttcccttg
     1621 cttttcagga ctttatgcct gcaacaaaga ggccgccgcg gaccctttcg gcgaccggaa
     1681 ttttccgccc tcaaaggccc cgaaaacaga cccacatcag gcgccatcac gcgcttacga
     1741 gacgccgcga acctcaatcc tgtcttgctg aaacgacgat cgctagaacg gaatctcgtc
     1801 gtcgagttcc ttgttgaagg acccgccgcc accgctgctg ctaagcggct tggtgcggcc
     1861 gaagtcgccg ccaccgcctc cggcgctctc gcccatgccg ccgccgtcgc gccgtccgtc
     1921 gagcatcgtg agcgtggagt tgaagccctg aaggacgacc tccgtggtgt agcgctcctg
     1981 gccggactga tcttcccatt tgcgcgtctg caacgcgccc tccaggtaga ccttggagcc
     2041 cttcttcaag tactgctcgg caatcttcgc caggccttcc gagaaaatca ccacgcggtg
     2101 ccactcggtt ttctcccgac gctcgccagt gttcttgtcg cgccagctat cggaggtcgc
     2161 cacgcgtaaa ttgacgatgg gccgaccgtc ttgggtatgc cggacttcag gatcggcgcc
     2221 gagattgccg atgagaataa ctttgttgat cgatccggcc atgttttgaa cctccccaca
     2281 gctgcgccgc cttagatgcc ttgaccaggc gggacagcac ctcgtaggca atcctgtact
     2341 ctaggcaggt cggcctcggc ctgccagcga acatcatccc ggcgccgaac ctgtccacaa
     2401 actccacaat ttctgccgct ggatctccag tgtacctgtt ttgttctcct tcgtctacca
     2461 tgtcgcgctc ttgagggttt caaaacacgg gccctatggc taagagaacg ggttgaaagc
     2521 ggctgctggc ggcgtgaggg gcctgatcgt cagcgcgcat aagcattacg aagggaatca
     2581 tgcagcgttc tgttgctaaa cgacggtcgc ccgtgcgccg caagaagacc aagcggcgcg
     2641 acccgcgcac caaatccttt gggttcaccg cgaggcagtg gccgttcatc gccacgctgg
     2701 tgggcaattg gatgtttgcg gctgcgttct tcgcgcttgc aaagaccttt tggcattgga
     2761 cgccggaggc ctggggcatt gccgaccgcc tggccctcgt catcaaggac gcggtgatcg
     2821 ccatcatccc gggcgtgatt gccatctgca tcgtcgccgc gcagcggctc gacccgtcca
     2881 tgttcgtcgg ccaggtgccg aagcccaact cgccggtgga catcaatacg aagttcatcc
     2941 tcaacacgtt cgagcagttc acggcttggt tcatcgccat ggcgggcctg gccatgtact
     3001 gcccgatcga ggaggcgcgt acgctgccga tcctgacggt gctgttcgtg cttggccgca
     3061 tcctgttctg ggtcggctac cacacgaacc catatctgcg cgccttcggc ttcggcatca
     3121 ccttctaccc gaccgtggtg gcctatgcct ggctggtgtt gatcatggtg ttcggcatcc
     3181 gcgtgccgct ttagggctca cctgctattc tgccattgca ggatcgcctc actcggccac
     3241 aaaagcataa tgacgttgag agtgaggccg tcgcggatca tcatgccgac gaacagttcg
     3301 agcgcgattg caagcagcac ggtcacccag accggcagcc atgcggccag gacgaagccc
     3361 acgatcatga acaggatgtc gctcacggag ttgagcacgc tgtcgccgta gtaatcgagc
     3421 gagaccgtca cctttcgata gtggttgatc acgtagtcgg tgttctcgat catctcccaa
     3481 cttgcctcga gcagcacggc cagaatgagc cgggtgccaa acgagctctt cggcgcgatg
     3541 agccagaaga aaaactagaa caggaagccg tggatgacgt gggacggcgt gtaccagtcg
     3601 aaaatgtgct gggagttctc cgagctcatg acatcgaaat gccagagctg cacacccccg
     3661 catttgcaga tgagcgggtg gcccatggcg tagcgggcgg caccggcaac aaggacgata
     3721 ccggcgatga tggcgatctg cgtacgcgtc atgctcatat gtgtctctat tggcggctgt
     3781 gggggcgcga ttcgcgccgg tccaatccgt tatgctggat tggcgcgctc gcgcctaggg
     3841 ctcggtagga gacaggaatg acgcaagacg aggcgctggc gctgctcaag acaggcgcca
     3901 gcatcttcct caccggccag cccggcagcg gcaagaccca caccgtgaac cgctatctcc
     3961 gcgacttggc gcaagccgga gtcgatgtcg cctataccgc ctcgaccggc atcgccgcca
     4021 cccacgggca tggcgtcacg atccatgcct ggagcgggat cggtgtgcgc gaggcgctga
     4081 cgcggcggga cttggatgtg ctggcgagca atcgccgtct cgctgcgcgc attgagaaga
     4141 cgagcgttct gattatcgac gaagtctcca tgctgccgcc gcgcgccttg acgctggcgg
     4201 atacagtctg ccgtcacatc cgggcgacga acgcgccatt cggcggcatg caagtagtgt
     4261 tggtcggcga cttcttccag ttgccaccgg tcatacgccg acgcttcgat tccagcgcat
     4321 tgccgttggc ggaagaagac gcagactcca gttcggcctt cgcgcatgcc tctgccgcat
     4381 ggcgcgatct cgatcccatg gtttgctatt tgtcggagca acatcggcaa gacgatcagc
     4441 cctttctgga tattctcgac gccatccgct cgaatgcctg cacgggcatt caccgggagc
     4501 gtctggccgc acgcatggtc gaccgcgatc agctaccgcc gagccgcacg cggctgttta
     4561 cgcataatgc cgccgtggac gagatcaacc agcagcaact cgcaaagctc gacggtgaga
     4621 ctcatacctt cgccatgacc gctaagggcg cgaagccctc gatcgatgcg ctgaagcgtg
     4681 gctgcctgtc tccggacgaa ttcgccgtaa agaagaatgc cgtggtgatg ttcaccaaga
     4741 acgatccggc cggtcgctac gtcaacggca cgctcgggac cgtggaggat ttcgattccg
     4801 aaacaggctc gcccatcgtg cgcacgcgca gcggcaaacg catcttcgcc gagcctgtga
     4861 cgtggaagat cgaagagaac ggcaaagagc gcgcgagcgt gacgcaaatt cccttgcggc
     4921 tcgcttgggc catcactgtg cacaagagcc agggcatgtc gctcgacgcc gcggtgatcg
     4981 acctgtcccg cgccttcgaa tacggccagg gctatgtggc cttgtcccgc ttgcgcagcc
     5041 tgtccggtct gcaccttttg gggctcaacg agcgggcgtt gcttgtgcat cccgcggcca
     5101 tcgacacgga tgccgaattt cggcgcgcgt cccacgacgc gcttcaggtc ctcgaacgcc
     5161 tcgggccgga cgaattggcc cagcggcacg cggcgtttct cgaggattgc ggcgcaagcg
     5221 ggaccgaagc cgcgcccaaa agctacgatg tggcgtcgct gcggaaagcc cacggcaagg
     5281 cctatgcccc ctggaccgag acggaagagc aggacttgcg ccgccgccac caggcgggcg
     5341 agagcgtggg ggccatcgcc gagcgattgg ggcgcaagcc cggcgccata aggtcgcggc
     5401 tgaagaagct ggccctgatc ccttgagtgc cgccacccct ccgcgcaccc ttgtccaccg
     5461 cccctttggc cactaagcct tgcaggacgg accccatcct ccgacattgc tgacagcatg
     5521 acctccaagt tcatctccat ccgcggcgcc cgggagcaca atctcaagac cgtcagcctc
     5581 gacctgcccc gcgagaagct cgtggtgatc acggggctgt ccggctcggg caagtcctcg
     5641 ctcgctttcg acacgatcta tgccgagggc cagcggcgct atgtggagag cctgtccgcc
     5701 tacgcccggc agttcctcga catgatgcag aagccggacg tggaccatat cgagggcctg
     5761 tcgccggcca tctccatcga gcagaagacc acgtcgaaga acccgcgctc cactgtcggc
     5821 acggtcacgg agatctacga ctatatgcgg ctcctctggg cgcgggtcgg catcccctat
     5881 tcgcccgcca cggggctgcc catcgagagc cagaccgtga gccagatggt ggaccgggtg
     5941 ctggagctcc ccgaaggcac gcgactttat ctgctggcgc cgatcgcccg cggccgcaag
     6001 ggcgaataca aaaaggaact cgccgagctc cagaagaagg gcttccagcg ggtcaagatc
     6061 gacaacgaat tctacgagat cgccgaggcc cctgccctca acaagaaact caagcacgac
     6121 atcgacgttg tcgtggaccg cgtcgtggtg cggaacgatg tgaccgcccg gcttgcggac
     6181 tcgctggaga ccgcgctgtc gatttccgac ggcatcgccg ccgccgaatt tgccgacgaa
     6241 cccgacaagc gcctgatctt ctcggcaaag tttgcctgcc ccgtctccgg tttcaccatc
     6301 gaggagatcg agccgcgcct gttctcgttc aacaacccct atggcgcctg cccggaatgc
     6361 gacgggctcg gcaccgaact gttcttcgag ccggagcttg tcgtgccgga cgaagcgcta
     6421 tcgctcaatc gcggagccat cgcgccgtgg acgcgcacgt ctgccacgtc gccctattac
     6481 cagcagacct tggagtcgct cgccaagcac tacaagcagt ccatgacgaa gccgtggaag
     6541 gacctgtcgg aggacttccg cgagatcgtg ctttatggct ccctggatga cgtcaatttc
     6601 gtctatgatg acggcgtgcg gcattactcc accgagaagc cttgcgaggg cgtcattacc
     6661 aacattgagc ggcgttggcg cgagacagaa tcgaactggg tgcgcgaaga gctcgagcgc
     6721 taccagtccg accagccttg cgaggcatgc acaggctatc gcctgaaacc ccaggcgctt
     6781 gcggtgaagg tcgcgggcct ccatatcggc gaggtccccg acatgtcgat ccgcgaggcc
     6841 ggcgcgtggt tcgaggcgct gccggagcac ctcacaagca agcagaacga gatcgccgcg
     6901 cgcatcctca aagagatccg cgagcggctc accttcctca acgatgtcgg cctcgaatat
     6961 ctcaccctct cgcgcgcctc aggcacgctg tcaggcggcg agagccagcg catccgcctc
     7021 gcctcgcaga tcggctccgg gctgaccggt gtgctctacg tgctcgacga gccttcgatc
     7081 gggctgcatc agcgcgacaa cgcgcggctg ctcgaaaccc tc
//
LOCUS       DBNHVU010000122         6925 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_567100, whole genome shotgun sequence.
ACCESSION   DBNHVU010000122 DBNHVU010000000
VERSION     DBNHVU010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6925)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6925)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6925
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_567100"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="AC16Y7_04840"
     CDS             complement(<1..833)
                     /locus_tag="AC16Y7_04840"
                     /inference="COORDINATES: protein motif:HMM:NF015662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR5708740.1"
                     /translation="MRETDKSDPAEARSARAYHTRCRYRFRLACLGFAVAFVLIGGRL
                     VSLGFAGTEKGRGGALDISTTVHRPDILDRNGQLLATDIRGATLYADPRRVIDRDELV
                     EKISSVFPDVNKPKLRKRLAGRSRFVRIKRELTPRQQSDVHELGLPGLGFIEEYRRVY
                     PVGATANHVVGLVDVDNRGLAGIEKFVDNNPQLTMMSPRTESGDETVTTSLDVGVQHV
                     LREELKNAMATYEAKAASGIVMDVHTGEVVGLASLPDFDPNHREQALDKDRINRIGFG
                     V"
     gene            complement(854..1198)
                     /gene="ftsL"
                     /locus_tag="AC16Y7_04845"
     CDS             complement(854..1198)
                     /gene="ftsL"
                     /locus_tag="AC16Y7_04845"
                     /inference="COORDINATES: protein motif:HMM:TIGR02209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HIR5708741.1"
                     /translation="MLRLVNVCLVLGLAALAYGIYQVKYEARALDERIAALHTEIEDE
                     RDALAVARAEWSLLNRPERIERLAKKYLELAPTQPRQLVVMDEVTERDLQSIGTPAEP
                     TKAAALKAQPIN"
     gene            complement(1233..2258)
                     /gene="rsmH"
                     /locus_tag="AC16Y7_04850"
     CDS             complement(1233..2258)
                     /gene="rsmH"
                     /locus_tag="AC16Y7_04850"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR5708742.1"
                     /translation="MMAGRGSNMAAVGGPARHIPVMLEEVLAALQPKDGDIVVDGTFG
                     AGGYTKAILDAADCKVIAIDRDAEAFRLGGQLAEAYPGRLIAMLGRYAEMEALAASEG
                     FESVDGVALDLGVSSMQLDQAERGFSFAKDGPLDMRMGPDGPTAADLVNSLSERELSD
                     IIYIYGEERRSRAIAKAIVAARTEAPITRTGELADLVVGVLGRKRDEPKHPATRTFQA
                     LRLYLNAELDELAKGLSAAENLLKTGGRLVVVTFHSLEDRIAKRFFASRSVAGPKGSR
                     HLPEAETQEFAPSFQLLNRRPLEPGNDEIQGNPRARSARLRAGERTAAPAHPLDLAKI
                     GVPQVPS"
     gene            complement(2255..2737)
                     /gene="mraZ"
                     /locus_tag="AC16Y7_04855"
     CDS             complement(2255..2737)
                     /gene="mraZ"
                     /locus_tag="AC16Y7_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR5708743.1"
                     /translation="MDQFVSTFTNRIDTKGRVSVPASFRNVLAKDGLDGIYCYPSLDA
                     PALDAGGQRLVDKINHLVEDLAPYSDERDQLATALFGTSEVLSIDQDGRTILPERLRE
                     HAGITSHVTFVGLGEKFQLWEPKSFENHLAEARNKVRDLRRLLGAGRRGSGEPEGERE
                     "
     gene            3222..4109
                     /locus_tag="AC16Y7_04860"
     CDS             3222..4109
                     /locus_tag="AC16Y7_04860"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5708744.1"
                     /translation="MLLAVLVVCPTAHGTEETPGFARLGPPDAPVKLFVEEKGEGSPL
                     LLIHGFGASTFTWREIAPALARQHRVIAVDLKGFGQSDKPFDERYSVFDQAELLKQLI
                     IDKDLRDLTLIGHSFGGGVALVLALDEDPQLKGRIKKLVLLDTIAYPQDIPVAFKMLD
                     MPVVSHVGVRMLPPAVQARLALRIAYLDNSKIDDDEVEMYAAPLRTAAGKHAIIHSAR
                     QIMPEDLEEISKRYASIELPTLIAWCDYDRVVPLDVGLKLRRTLPNATLEIIDSCGHM
                     PHEEQPEATLELIEDFLDG"
     gene            4256..6436
                     /gene="katG"
                     /locus_tag="AC16Y7_04865"
     CDS             4256..6436
                     /gene="katG"
                     /locus_tag="AC16Y7_04865"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018237447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HIR5708745.1"
                     /translation="MDAKVEGGCPVSHNRATTNRDWWPNQLNLKILSQHSSKVDPMGE
                     AFDYAEAFKTLDLDAVKADLVALMTDSQDWWPADYGHYGPLFIRMAWHSAGTYRTADG
                     RGGGGTGNQRFSPVNSWPDNASLDKARRLLWPIKQKYGKKLSWADLLILAGNCALESM
                     GLKTFGFAGGRADIWEPEEDIYWGSEAEWLGDRRYSGDRELERPLAAVQMGLIYVNPE
                     GPDGNPDPIASGRDIRETFARMAMNDEETVALTAGGHTFGKCHGAAPDSYVEREPEAA
                     GIEMQGLGWHSTYKSGKGVDAITSGLEGAWTPTPTQWDMSYFDMLFGHEWELVKGPGG
                     GHQWAPKNAAEKDLAPRADDPSKKVPTMMTTADMAMRMDPAYEKIARRFHANPEEFAD
                     AFARAWFKLTHRDMGPKSRYLGNEVPDEDLIWQDPIPAVTHKPIDDKDIADLKAKILA
                     SGLTTSELVSTAWASAATFRGSDMRGGANGARIRLAPMKDWEVNQPEQLARVLAKLED
                     IQSSFNDAASGGKQVSLADLIVLGGCAAVEKAAKDAGHDVSVPFSPGRGDATQEQTDI
                     ESVNFLEPVADGFRNYLKTHYTVSPETLLVDRAQLLTLTAPEMTVLVGGLRVLGANHG
                     GSKHGVFTDRPGQLTNDFFVNLLDMGTAWKAVSDEEEEFEGRDRNTDELKWTGTRADL
                     VFGSNSQLRALAEVYATDDSKDAFVHDFVAAWTKVMNADRFDIA"
     gene            complement(6582..>6925)
                     /gene="rnpB"
                     /locus_tag="AC16Y7_04870"
     ncRNA           complement(6582..>6925)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="AC16Y7_04870"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1325 a         2200 c         2160 g         1240 t
ORIGIN      
        1 tagacgccaa agccgatgcg attgatcctg tccttgtcga gcgcctgctc gcggtgattg
       61 ggatcgaaat cgggaagtga agccagcccc accacctctc cggtgtgcac gtccatgacg
      121 atgccggagg ccgccttggc ctcgtaggtg gccatggcgt tcttcagttc ctcgcgcagc
      181 acgtgctgca caccgacatc cagcgacgtc gtgacggtct catcgcccga ctctgtgcgg
      241 gggctcatca tggtgagctg cggattgttg tcgacgaact tctcgatgcc ggccaggccg
      301 cgattgtcga cgtcgaccag tcccacaacg tgattggccg tggcgccgac gggatagacg
      361 cgccggtact cctcaatgaa accgagaccc ggcaggccaa gctcgtgaac gtcggactgt
      421 tgccttgggg tcagctcgcg cttgatgcga acgaagcgac tacggcccgc taggcgcttg
      481 cgcagcttcg gcttgttgac gtccggaaag acgcttgaaa tcttctcgac gagttcgtcg
      541 cgatcgataa cgcgtctcgg atcggcataa agagtcgcgc cacgaatgtc ggtcgcgagc
      601 aattgcccgt tgcggtccag aatgtcggga cggtgtacgg tggtcgagat gtcgagagcc
      661 ccgccgcggc ctttctccgt tccggcaaag ccgagcgaga ccagccgtcc gccgatcagc
      721 acgaaagcga ccgcgaagcc aaggcacgcc agccggaaac gataccggca gcgtgtgtgg
      781 taggcgcgcg cggagcgcgc ttcggcggga tcggatttgt cggtctcgcg caaagttctg
      841 ctggtccgta aagttagttg atgggttggg ccttgagcgc cgcggccttg gtgggttccg
      901 ccggcgtacc gatgctctga agatcgcgct cggtgacttc gtccataacc acgagttgcc
      961 gcggctgggt gggtgcaagc tcgagatatt tcttggcgag ccgttcgatg cgctcgggcc
     1021 tgttgagcag gctccattcg gcgcgtgcca ccgcgagcgc gtccctctcg tcctcgattt
     1081 ccgtgtgcag ggcggcaatg cgctcatcga gcgcgcgggc ctcgtacttc acctgataga
     1141 ttccataggc cagcgcggcg aggccgagga cgaggcagac attcactaaa cgcaacatgg
     1201 tactcaaaac gcgtgaggct cacgcgctcc ccctaactcg gcacttgcgg caccccaatt
     1261 ttcgccagat ccaacgggtg ggctggcgcc gccgtacgct ccccggctct cagccgggcc
     1321 gaacgggccc gcggattccc ctgaatctca tcgttaccgg gttctaaagg acgacggtta
     1381 agaagctgga agcttggcgc aaattcttgc gtttcggcct cgggcaggtg ccgggagccc
     1441 ttggggcctg cgacgctgcg gctggcgaag aaccgcttgg cgatgcggtc ttccagcgaa
     1501 tgaaaggtca ccacgaccag ccggccacct gttttcagca ggttttcggc ggcggacagg
     1561 cccttggcca gctcatccag ctcggcgttg agatagagcc gcaaagcctg aaacgtgcgc
     1621 gtggcgggat gcttgggctc gtcccgctta cggcccagaa cgcccacgac gaggtccgca
     1681 agctcgcctg tacgcgtgat cggggcctcc gtgcgggccg cgacgatggc cttggcgatg
     1741 gcgcgcgagc gccgctcctc gccataaatg taaatgatat cagagagttc tcgttcagac
     1801 aggctattca cgagatccgc cgccgtgggg ccgtcgggtc ccatgcgcat gtcgaggggg
     1861 ccgtccttgg caaaggaaaa gccgcgctcg gcctgatcga gctgcatcga ggacacgccg
     1921 agatcgaggg ccaccccgtc gacgctctcg aacccctcgc tggcggcaag cgcttccatc
     1981 tcagcgtagc ggcccagcat ggcgatgagc cggccgggat aggcctcggc aagctggccg
     2041 ccaaggcgga aggcctccgc atcgcgatct atggcgatga ccttgcagtc ggcggcgtcg
     2101 agaatggctt ttgtatagcc tccggcgccg aaggtgccgt cgacgacgat atcgccgtcc
     2161 ttaggctgaa gcgcggccag aacctcttcg agcatcacgg gaatgtggcg ggccggtccg
     2221 ccaacggcag ccatattgct gccgcgaccc gccatcattc ccgttctccc tctggctcgc
     2281 cgcttccgcg gcgccccgcg ccgagtaacc tgcgaaggtc gcgtaccttg ttgcgcgcct
     2341 ccgcgagatg attttcgaag ctcttgggct cccagagctg aaacttttct cccagcccga
     2401 caaaagtgac gtggctcgta atccccgcgt gttcgcgcaa gcgttccggg agaatcgtac
     2461 ggccgtcttg atcgatggag agcacttcgc tggtgccgaa gagcgcagtc gcgagctgat
     2521 cgcgctcatc ggagtaaggc gcgagatctt cgaccagatg attgatttta tcgacgaggc
     2581 gctgcccgcc ggcgtcgagc gcgggggcgt caagagacgg atagcaatag atgccgtcga
     2641 gcccgtcctt ggcgaggacg tttcggaagg acgcagggac cgaaacgcgg cccttggtgt
     2701 cgatcctatt tgtgaatgtc gagacaaact ggtccatctc ccccctcttg cgcttcgaag
     2761 tcgacggaac tgaacaagga caaacggcac ttcggcgctt ggcacccgga ctttgagaaa
     2821 aatggggtgg aggaaacggc gcgaaatccc cccgtggcct catgggaagc gctgccagct
     2881 tcccccaccc ctattcaccg aacgggcatt aatgggatag catgggataa catgggcgtc
     2941 aatggaatcg accgggttgc accctcaatt ctgtgggaaa gcgcgtctcg ctcgacgacc
     3001 caatgtccgc aaggctcaat tgccgcttga gcgcgaagca cgtagaaagg cgcggaacgt
     3061 taatgcgtcg gacctcgtga tccggaacgc tagagacggc cttaggattt gcgcattcga
     3121 ggcgtctgct gtgcctggga caggtggcca agggcctttt gcggcgggac tggcgaaata
     3181 tggcaaacag aagtttaaga cgcgcatttg gcccagtcgc catgctgctc gctgtcctcg
     3241 tcgtttgccc cacggcccac ggcacggaag aaacgcccgg gttcgcgcgg ctcggaccgc
     3301 ccgacgcacc ggtgaagctg ttcgttgagg aaaagggcga aggctcgccc ctgctcctga
     3361 tccacggctt cggcgcaagc acgttcacct ggcgggagat cgcgcccgcg ctcgcacgcc
     3421 agcaccgggt gatcgccgtc gacctcaagg gcttcggcca gtccgacaag cccttcgacg
     3481 agcgctattc cgtcttcgac caggccgagc tgctcaagca actcatcatc gacaaggatc
     3541 tgcgcgacct cacgctgatc ggtcactctt tcggtggagg cgtggcgctc gtgctcgctc
     3601 tcgacgagga cccgcagctc aagggccgca tcaagaagct cgtgctgctc gacacaattg
     3661 cctatcccca ggacattccg gtggccttca aaatgctcga catgccggtc gtctcccatg
     3721 tcggggtgcg catgctgccc ccagccgttc aggcgcgcct ggcgctgcgc atcgcctatc
     3781 tggacaacag caagatcgac gacgacgagg tcgagatgta cgccgcgccg ctcaggaccg
     3841 ccgccggcaa gcacgcgatc attcacagcg cacggcagat catgccggaa gacctggaag
     3901 agatatcgaa gcgctacgcg tcgatcgaac tgccgaccct catcgcctgg tgcgactacg
     3961 accgcgtcgt cccgttggac gtggggctga agctgcgacg gacactgccg aacgcgacgc
     4021 tcgagatcat cgacagctgc gggcacatgc cccacgaaga gcagcccgaa gcgacgcttg
     4081 agctgatcga agactttctc gacggctgac gcccggcacc actctctccg acatcggaga
     4141 taagttttgc ttatcgcacc aatagggaaa tacgatttgc ccttatggtg aaccgcgcgc
     4201 aatctccgct ctgagtacca agagccaaat caaggccaat gatcggagat gagacatgga
     4261 tgcgaaagtc gagggcggct gccccgttag ccacaaccga gccaccacca accgggactg
     4321 gtggcccaac caactgaacc tcaagattct gagccagcac tcgtccaagg tcgatcccat
     4381 gggcgaggcc ttcgactatg ccgaagcgtt caagacgctc gacctcgatg cagtgaaagc
     4441 agaccttgtc gcgctaatga cggatagcca agactggtgg ccggccgatt acggtcacta
     4501 cggaccgctg ttcatccgca tggcctggca cagcgcgggc acctaccgca ccgctgacgg
     4561 acgcggcggc ggcggcaccg gcaaccaacg cttctcgccg gtaaacagct ggccggacaa
     4621 tgcaagcctg gacaaggcgc gccgcctgct ttggccgatc aaacagaaat acggcaagaa
     4681 gctctcctgg gccgatctgc taatcctggc cggcaactgc gcgctggagt cgatgggtct
     4741 caagacgttc ggctttgcgg gtggccgcgc ggatatctgg gagccggaag aagacatcta
     4801 ctggggctcg gaggccgagt ggctcggcga ccggcgctat tcgggcgacc gcgagctcga
     4861 acggccgctc gccgccgtgc aaatgggtct catctatgtg aacccggaag ggccggacgg
     4921 caatcccgac ccgatcgcct cgggccgcga catccgcgag accttcgccc gcatggcgat
     4981 gaacgacgag gaaaccgtcg cgctcaccgc tggcgggcac acattcggga agtgccatgg
     5041 cgccgcgccc gactcttatg tggagcgcga accggaagcg gccggcatcg agatgcaagg
     5101 cctcggctgg cacagcacct acaagagcgg caaaggtgtc gacgccatca ccagcggcct
     5161 cgaaggcgcc tggaccccga cgccaacgca gtgggacatg agctatttcg acatgctgtt
     5221 cggtcacgag tgggaactgg tgaaaggccc cggcggcgga catcaatggg cgccgaagaa
     5281 tgcggccgag aaagacctcg caccgcgcgc cgacgatccg tcgaagaaag tgccgaccat
     5341 gatgaccacg gccgacatgg ccatgcgcat ggacccggca tacgagaaaa tcgcgcggcg
     5401 ctttcacgct aacccggaag aattcgccga cgcgttcgcc cgcgcctggt tcaagctgac
     5461 ccatcgcgac atgggcccca agtcccgcta tctcggcaac gaggtaccgg acgaggatct
     5521 catctggcag gacccgatcc ccgccgttac gcacaagccg atcgacgaca aggacatcgc
     5581 cgatctcaag gccaagattc tggcgtccgg tttgacgaca tccgagctgg tctcgacggc
     5641 ttgggcgtcg gccgccacgt tccgcggctc ggacatgcgc ggcggcgcga acggcgcacg
     5701 cattcgtctt gcgccgatga aggattggga ggtcaatcag ccggagcaac tcgcacgcgt
     5761 gctcgccaag ctggaagaca tacagtcctc gttcaacgac gcggcaagcg gcggcaagca
     5821 ggtgtcgctc gccgatctca tcgtcctggg cggctgtgcg gcggtcgaga aggctgccaa
     5881 ggacgcagga cacgatgtta gcgtgccctt ctcgcccggc cgcggcgacg ccacgcagga
     5941 gcagacggac atcgagtccg tgaacttcct tgagcccgtt gcggacgggt tccgcaatta
     6001 cctcaagacg cactacacgg tttcgccgga aacactgttg gtcgaccggg cacaattgct
     6061 gacactcacg gcgcccgaga tgaccgtgct tgtcggcggc ttgcgtgtcc ttggcgcgaa
     6121 ccacggcggc tccaagcatg gcgtcttcac cgaccggccc ggacaactca ccaacgactt
     6181 cttcgtcaac ctgctcgaca tgggcacggc gtggaaggcc gtttcggatg aggaagaaga
     6241 gttcgaggga cgcgaccgca acacggacga gttgaaatgg accggcacgc gcgccgatct
     6301 cgtcttcgga tcgaactccc aactccgagc gctggcggaa gtctacgcaa ctgacgattc
     6361 gaaagacgct tttgtgcacg acttcgtggc ggcttggaca aaggtgatga acgccgaccg
     6421 cttcgacatt gcctagtcgc ggcagcgagt agtgcgaagt gccaccttcc ccggcgcttc
     6481 gatgcaaaac ggccggtctg attttcacgc cggccgtttt ctatttgcgc tgctatataa
     6541 gttggaggcc ggcgcgaggt ttttcctcgc accggcccta acatgtcacc aagcctgtaa
     6601 gccgggttct gtccctcgcg agcgagggga tggccattca tctgggacgc atcttgcgat
     6661 gcgcctcctg caaccaaccc gggcggtcag tctggaaaca gacttgggcg attactcgcc
     6721 cgcgccgccc ctattcggtc ttgctcccgg tggggtttgc cttgccgcct ttgttgccaa
     6781 agacgcggtg cgctcttacc gcaccctttc acccttaccc tcgtatgagg gcggtttgct
     6841 ttctgtggca ctttccctgg ggtcgccccc gccggaggtt atccggcacc gtgcttccaa
     6901 ggagcccgga ctttcctccc cgcct
//
LOCUS       DBNHVU010000123         3408 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_493093, whole genome shotgun sequence.
ACCESSION   DBNHVU010000123 DBNHVU010000000
VERSION     DBNHVU010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_493093"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..1127
                     /locus_tag="AC16Y7_04875"
     CDS             186..1127
                     /locus_tag="AC16Y7_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HIR5708746.1"
                     /translation="MEHAIVFLDRESVGANVREASFPHNYTEHQSTWTPEDIVERLKD
                     ASIAIINKVPMREDVLKQLPKLKLIAVAATGTDVVDKAYCKANGITVVNIRNYAFNTV
                     PEHVIALMFALRRNLMAYIEDTRNGRWAEVDQFCFFDHPIRDIAGSTMGIVGYGAIGK
                     AVAKRAECLGMKILPYDVFPQDGLVDLETVLKESDVITLHCPLTPETKNMIGEKELKM
                     MKPTSILINTARGGLVDEAALAKALQDGTIAGAGFDVLTKEPPKEGNVLLNLKMPNFI
                     VTPHVAWASREAMQILADQLIDNIEAFVAGNPQNVVE"
     gene            1147..2004
                     /locus_tag="AC16Y7_04880"
     CDS             1147..2004
                     /locus_tag="AC16Y7_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent
                     methylenetetrahydromethanopterin/methylenetetrahydrofolate
                     dehydrogenase"
                     /protein_id="HIR5708747.1"
                     /translation="MKKLLFLFDTDQTPSVFDTVVGYDGGADHVTGYANVTPDNVGAL
                     VDGTIFTRGGKDKQNTAIFVGGGNMAKGEELYEKVKKSFFGPFRVSVMLDSNGSNTTA
                     AAGVALLAKGRSLKGKKALVLAGTGPVGMRAAGFLGMEGADVTITSRTKDRAEAAAKV
                     IEKRFGIKVEGAAGATDEERAEAAKDANVIFAAGAIGIQLLPESAWKDNSNVELLADV
                     NAQPPLGVEGIKATDKGAEYGDKIAFGALGIGGLKLKLHRACIAKMFESAEAAFDAEE
                     IYALAKEMA"
     gene            2007..2639
                     /gene="fchA"
                     /locus_tag="AC16Y7_04885"
     CDS             2007..2639
                     /gene="fchA"
                     /locus_tag="AC16Y7_04885"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004477 - methenyltetrahydrofolate
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HIR5708748.1"
                     /translation="MTQIKDTAIEPFLDALASSAATPGGGSAAAILGGMGAALVSMVC
                     NLTIGKKKYADVEGDMKDLLAKAEDLRHRLTGMIQEDVRAFDTVMGAYGMPKETDADK
                     AARDAAIQDALKQATEVPIRCCRLAREVIDLALTASEKGNLNVISDAGVGVLSANAAL
                     RSAALNVYINVKMISDKSFTDGKLSELENLLAGAEATTEKAYDIVKGKVS"
     gene            2703..>3408
                     /locus_tag="AC16Y7_04890"
     CDS             2703..>3408
                     /locus_tag="AC16Y7_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR5708749.1"
                     /translation="MDVHEYQAKELLGSFGVPVPKGAVAFSPDQAVYAATELGGWSWA
                     VKAQVHAGARGKAGGIKICKTYHEVREAAQELLGKRLVTIQTGPEGKPVQRVYIEAAE
                     PFERELYLGYVLDRKAERIRVIASQQGGMEIEEIAKNDPDAILQVIVEPAVGLQSFQA
                     RELAFQLGLSIKQAQSCVSTIMNAYRAFRDCDAQMLEINPLVVAKDDRVLALDAKMSF
                     DDNALFRRHNVADMYDP"
BASE COUNT          718 a          995 c         1097 g          598 t
ORIGIN      
        1 ccggggcgcg caaggtatgg ccgagactcg tctcgcggcc gagtagacgg ttttgatgcg
       61 aaggccgcga acgctgcctt cgatagacgt gcgttgactg tcgcgcctgg cgcaaaaatt
      121 tgcaccgggc cggatttctt ttttggtggg gcgtgccccc gcagcccgtc aaagggggga
      181 gttcagtgga acacgcgatt gtcttcctcg accgggagtc ggtcggcgcc aatgtgcgcg
      241 aggccagctt tccgcataac tacaccgagc accagtcgac ctggacgccc gaggatattg
      301 tcgagcgcct caaggacgcg tccatcgcca tcatcaataa agtgccgatg cgcgaggacg
      361 tgctgaagca gttgccgaag ctgaagctga ttgccgtcgc cgcaaccggc acggatgtgg
      421 tcgacaaggc ttactgcaag gcgaacggca tcaccgtcgt caatatccgc aactacgctt
      481 tcaataccgt gccggagcat gtcatcgcgc tgatgttcgc gctgcgccgc aatctaatgg
      541 cctacatcga ggatacgcgg aacggtcgct gggcggaggt cgatcagttc tgtttcttcg
      601 accatccgat ccgcgacatc gccggctcta ccatgggcat tgtcggctac ggcgccatcg
      661 gcaaggccgt tgccaagcgc gccgagtgcc tgggcatgaa gatccttccc tacgacgtgt
      721 tcccgcaaga cgggctcgtc gacctggaga cggtgctgaa agaaagtgac gtcatcacgc
      781 tgcattgtcc gctcacgccc gagaccaaga acatgatcgg cgaaaaagag ctgaagatga
      841 tgaagccgac atcgatcctc atcaacacgg cacgcggtgg tctcgtagac gaggcggcgc
      901 ttgccaaggc gcttcaggac ggcaccatcg ccggcgccgg cttcgacgtg ctcaccaagg
      961 agccgccgaa agaaggcaac gtcctgctta atctgaaaat gccgaacttc atcgtcacac
     1021 cgcacgtggc gtgggcgagc cgcgaggcca tgcagatcct ggccgatcaa ctcatcgaca
     1081 acatcgaggc gttcgtcgcg ggcaacccgc agaacgtcgt cgaatagatt ttgagggcaa
     1141 gttgaaatga agaaactgct gttcctgttt gacaccgatc aaacgccgag tgtgttcgac
     1201 acggtcgtcg gttatgacgg cggcgccgac cacgtcacgg gctatgccaa tgtgacgccc
     1261 gacaatgtgg gcgcactggt cgacggcacc attttcacgc gcggcggcaa ggataagcag
     1321 aacactgcga tcttcgtcgg cggtggcaac atggccaaag gcgaagagct gtacgagaag
     1381 gtgaagaaga gcttcttcgg tcccttccgc gtctcggtca tgctcgacag caatggctcc
     1441 aacacgacgg ctgcggctgg tgtggcactg ctcgccaagg gacgctcgct caagggcaag
     1501 aaggcgctgg tgctcgcggg caccgggcct gtcggcatgc gcgccgccgg tttcctcggc
     1561 atggaaggcg cggacgtgac aatcacctcg cgcaccaagg atcgtgctga ggccgctgct
     1621 aaagtcatcg agaagcgctt cggcatcaag gtcgaaggcg cagccggtgc cacggacgag
     1681 gaacgcgccg aagcagccaa ggacgccaat gtcatcttcg cggctggcgc catcggcatc
     1741 cagctcctgc cggagtcggc ttggaaggac aattccaatg tcgagcttct ggccgacgtg
     1801 aatgcccagc cgccgcttgg cgtcgagggt atcaaggcca ccgacaaggg cgcggagtat
     1861 ggtgacaaaa tcgcctttgg tgcgctcggc attggcgggc tgaagctgaa acttcatcgt
     1921 gcttgcatcg ccaagatgtt cgaaagcgcc gaggcggcat tcgacgccga ggagatctac
     1981 gcgctggcaa aagagatggc ttaggcatga cccagatcaa agacacggcg atcgagccct
     2041 tccttgatgc gctcgcttcg agtgcggcga cccccggagg cgggagcgcg gccgcgattc
     2101 tcggcggcat gggcgctgcg cttgtcagta tggtctgtaa cctgaccatc ggcaaaaaga
     2161 agtacgccga tgtggaaggg gacatgaagg acttgctggc caaggccgaa gatttacgtc
     2221 accggctgac cggcatgatc caagaggacg tgagggcgtt cgacacggtg atgggtgcgt
     2281 acgggatgcc gaaggaaacc gacgccgaca aagccgcgcg cgacgcggcg atccaggatg
     2341 cgctgaagca agcgacggag gttccgatcc gctgctgccg cctggcgcga gaggttatcg
     2401 acctggcgtt gacggcctcc gagaagggca acctgaacgt gatttctgac gcgggcgtcg
     2461 gtgtcctgtc cgcgaatgct gcactgcgaa gcgcggcgct caatgtctac atcaacgtga
     2521 aaatgatctc ggacaagagc tttaccgacg gtaagctgag cgaactggag aaccttctcg
     2581 ccggcgctga ggccacgacc gagaaggctt atgacatcgt caaaggcaaa gtgagctgat
     2641 gccttagggc gcggctcgtc aacgaaaaga gcccgccgaa gacttacgaa gggagaaacg
     2701 acgtggacgt tcacgagtat caagcaaaag agcttctggg cagcttcggc gtgccggtgc
     2761 cgaagggtgc cgtagccttc agccccgacc aggccgtcta tgcggcgacc gagcttggcg
     2821 gttggagttg ggccgtgaag gcgcaagtcc atgctggtgc gcgcggcaag gccggcggca
     2881 tcaagatttg caagacctac cacgaagtgc gcgaggcggc gcaggagctg ctcggcaagc
     2941 gtctggtaac gatccagacc ggccccgagg gcaagcccgt gcagcgtgtc tatatcgagg
     3001 cggccgagcc ctttgagcgg gagctttatc ttggctacgt gctcgaccgt aaggcggagc
     3061 gcatccgcgt gatcgcctcg cagcagggcg gtatggagat cgaagagatc gccaaaaacg
     3121 acccggacgc cattcttcag gtcattgtcg agccggccgt tggccttcag tccttccagg
     3181 cgcgcgaact ggccttccag cttggtcttt ccatcaagca ggcgcagagc tgcgtgtcga
     3241 cgatcatgaa cgcctatcgc gcattccgcg attgcgacgc tcagatgctg gagatcaacc
     3301 cgctggtcgt ggccaaggac gatcgcgtgc tggcgctcga cgccaagatg tcgtttgacg
     3361 acaacgcgct gttccgccgc cacaacgtcg ccgacatgta cgacccgg
//
LOCUS       DBNHVU010000124        11895 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1905814, whole genome shotgun sequence.
ACCESSION   DBNHVU010000124 DBNHVU010000000
VERSION     DBNHVU010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 11895)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11895)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11895
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1905814"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="AC16Y7_04895"
     CDS             <1..263
                     /locus_tag="AC16Y7_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012709165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HIR5708750.1"
                     /translation="QAAERHATPLTLCGEMAGRPLEAMALIGLGFRSISVAPASVGPI
                     KAMVLSLDAGQLAERLDALLAGGETSLREDLKRFAAENGVQA"
     gene            474..1565
                     /locus_tag="AC16Y7_04900"
     CDS             474..1565
                     /locus_tag="AC16Y7_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5708751.1"
                     /translation="MPAGYDAEVADLFQDFRVAISKNTGSNLTEADLAARIATPVEVV
                     QALEQGAIYALPPWPETARVVNAYGSLLNLDTRPLLRRIYAQIEAGVVELGPKPVPDV
                     PVMPPQAGGDAALGGAAPAQPQRQPQPQPQGMPQAAPGQPGWQNGPAQPQPQAPHPQT
                     PLAPNWPGGAPQAPHAGMPGAPGQPPQQAPAAPAWSNGPQGVPQPQVPNPAAYQQPPQ
                     AYPPEGALPLQGEDASFEDAAMGGDAAMGDAGRGDAAMGSDAAMMDPNLGDPAMSDPA
                     MGDPAMGGAEMGDPAAFGAEPEPKKKRPALLKWGIAALVASVVLVGLWMFLGDSSDSS
                     SSSGSFASDPVLDPDDPRSRKADRLPSKF"
     gene            1584..2672
                     /gene="prfA"
                     /locus_tag="AC16Y7_04905"
     CDS             1584..2672
                     /gene="prfA"
                     /locus_tag="AC16Y7_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018901546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR5708752.1"
                     /translation="MTTIPAEKLDKLVARWEAVQGSLASGADQESYVQLSREFAELDP
                     IVATINALRAALAEREGLQEILDDRASDPDMTALAEDEIGGLAEKIETLEHELKVQLL
                     PKDAADDKSAILEVRAGTGGDEAALFAGDLFRMYQRYADRHGWKVNIMSASESATGGY
                     KEIIANITGKGVFARMKYESGVHRVQRVPETEAQGRIHTSAATVAVLPEAEDVDVQID
                     EKDLRIDVFRASGPGGQSVNTTDSAVRITHLPTGLIVTQQDEKSQHKNKAKAMKVLRA
                     RLYDAEREKLDAERAQDRRSQIGSGDRSQRIRTYNFPQGRVTDHRIGLTTHQLQLVLQ
                     GEPALDEVVDALITEDQTSQLAAMEQES"
     gene            2672..3538
                     /gene="prmC"
                     /locus_tag="AC16Y7_04910"
     CDS             2672..3538
                     /gene="prmC"
                     /locus_tag="AC16Y7_04910"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR5708753.1"
                     /translation="MVSIDAVRNETARTLRAAGIDSPALDARLLLCEAVGVTHEALVA
                     RGREDLPPHAAERLSGYLARRLGGEPVSRIKGKREFYGRDFLIDTHTLDPRPDTETLI
                     TAALDLVAREGRTGSPLSVLDLGTGSGCILLTLLAELPEAQGLGTDIAPGALEVAARN
                     ASCLGLELRARFLGTSWLEGLKGGFDLIVSNPPYIARGEIGGLAREVAVHDPMAALDG
                     GADGLDAYRTIAARVGDFLSPGGHLLVEIGAAQAEPVSRIFHHSGLGIDREGILSDLA
                     GRPRCVLARSPF"
     gene            3948..4184
                     /locus_tag="AC16Y7_04915"
                     /pseudo
     CDS             3948..4184
                     /locus_tag="AC16Y7_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006014194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
     gene            4710..7313
                     /gene="clpB"
                     /locus_tag="AC16Y7_04920"
     CDS             4710..7313
                     /gene="clpB"
                     /locus_tag="AC16Y7_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIR5708754.1"
                     /translation="MDFEKLSDRLKGFLQAAQTIALRDGNPQITPEHLLKALLDDEEG
                     MAAGLIARAGGDPKTALARTDAALAKLPKVSGSGATQPQMTPGLARVLDQAEKIATKA
                     GDSFITVERALQALAMTKEGETADILKDAGVTPQSLNETINDLRKGRTADSAGAEQQY
                     DALKKYTRDFTEAAEKGEMDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVKGDVPDSLKDKKVLSLDMGALIAGAKYRGEFEERLKAVLSEIEASEGQIV
                     LFIDEMHTLVGAGKADGAMDASNLLKPALARGQLHCVGATTLDEYRKHVEKDAALARR
                     FQPVFVPEPTVEDTISILRGLKEKYELHHGVRISDAALVTAATLSNRYITDRFLPDKA
                     IDLVDEAASRLRMQVDSKPEELDELDRRLMQLKIEREALKKETDNASKDRLEKLEKEI
                     VNLEESANVLSQRWQEEKDKLGAAQKIKEDLDHARIELDKAQRDGDLTKAGELAYGVI
                     PDLEAKLANIEEEEGKAGSLAQEVVEPDMIAAVVSRWTGIPVDKMLEGEREKLLQMET
                     EIAKRVVGQKEAVQAVSTAVRRSRAGLQDPNRPLGSFMFLGPTGVGKTELSRALAEFL
                     FDDEHAILRVDMSEYMEKHAVARLIGAPPGYVGYEEGGALTEAVRRRPYQVILFDEIE
                     KAHPDVFNVLLQVLDDGRLTDGQGRTVDFRNTLIIMTSNLGSEFLVNQKEGEDSDAVE
                     DQVMEVVRGHFRPEFLNRVDEIILFHRLAREDMGQIVDIQMKRLLKLLADRKITLTID
                     DDAKTWLGNKGYDPAYGARPLKRVIQKYVQDPLAEQILEGKIRDGATVKVGVKDGGLT
                     FNGEAADATGG"
     gene            complement(7360..8925)
                     /locus_tag="AC16Y7_04925"
     CDS             complement(7360..8925)
                     /locus_tag="AC16Y7_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003599394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute symporter family protein"
                     /protein_id="HIR5708755.1"
                     /translation="MTTAIWLFLLFVAFTLLITWWSARRSGGASAYFAAGRRIKAWQN
                     GLAIAGDYMSAASFLGIAGMIALKGYDGFMYSVGFLVAYLTVLLIVAEPLRNSGKFTM
                     ADVLAYRLKARPVRAMAALSTLTVTIFYMIAQMVGAGSLVKLLLPGVSYELAVIGVGV
                     LMIIYVVFGGMLATTWVQITKAILLLAGSVMLSILVMAQFGYSFGSFFEAVSHVTWID
                     PKTGAQVTQNFLQPGLAFKGTLGAIDLISLGIALVLGTAGLPHILIRFYTVPDARTAR
                     LSVVWAMVAIGAFYLMTTFFGFGAATLLTPANIPDQNMAAPLLAQHLGGDIFFAFISA
                     VAFATILAVVAGLTISASTSFAHDFYTNVIHAGRERAPGEEVRVARIAAFVVGATAIL
                     LAIGLRSTNVAFLVGLAFAVAASANLPPMVLALFWKRFDTAGACWGLGVGLVSSIALI
                     LLSPSIMGTDGTTLIAGEPIFPLRNPGIVSVPLGFAAAIVATLLTRERAAEEKFAEFS
                     VRANTGLGAEKAV"
     gene            complement(8922..9362)
                     /locus_tag="AC16Y7_04930"
     CDS             complement(8922..9362)
                     /locus_tag="AC16Y7_04930"
                     /inference="COORDINATES: protein motif:HMM:NF016244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HIR5708756.1"
                     /translation="MSKKDLDLTTSVSAETDVTRVGRTEAPPPHEQTADEDIDVVDWD
                     RVASTNDFKTLVDLRARFLIPATIFFLAYYFTLLVLVGFFPEVMSRTVSWLGPVNLAY
                     LFALSQFAMVGLVAYAYLRHAKRFDALAAKIRREALVSSGSKTT"
     gene            9657..9902
                     /locus_tag="AC16Y7_04935"
     CDS             9657..9902
                     /locus_tag="AC16Y7_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708757.1"
                     /translation="MAETKKPNIGLALSGGGFRATLFHLGLIRFLRDAGLLSNVTQPP
                     NAVALGTFERKPRRLLGTNVWRSSATRSSPAHRPPPS"
     gene            10019..10555
                     /locus_tag="AC16Y7_04940"
     CDS             10019..10555
                     /locus_tag="AC16Y7_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HIR5708758.1"
                     /translation="MNVLIAYGTTEGQTRKIAETAAAHLRGAGHEVALYDTADPGPPV
                     ELDGFDASVIAASVHQEQHQPVVKRFAAAHCQSLNSMPSAFLSVSLSAALEETRGEAQ
                     AYVDRFLAVTGWQPAMTLLLGGALRFTEYDFFQEQIVKFIVMKRGVVSETESDREFTN
                     WDALSSFLDAFLMTAEAR"
     gene            complement(10650..>11895)
                     /locus_tag="AC16Y7_04945"
     CDS             complement(10650..>11895)
                     /locus_tag="AC16Y7_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR5708759.1"
                     /translation="HLASHRPAEFERPRLMPNTTVLRKVLDEDEASTEKDIARAETKV
                     ITVGSGDNLMGVLLKSGTDKEMASAIIDSLDAVFAPEDLKPRQKMDFRLVPSAANPEA
                     KDVVRVSVFDRKGNHIASATRNRDGDYLASTTAPVDEVVITASDRASLYESFYTAALE
                     QKLPADTIVKLLRVHSYDVDFKQKVRSGDTFEMFFDGEGVSGSDAGELLYTSMTVGGE
                     TRKFYRFRTPDNTIDFYDENGSSAKKFLMRNPVKGGRFTSGFGNRRHPLLRRIRMHTG
                     VDWAAPSGTPILAAGDGTIEMAERHGGYGNYIRIRHANGFATAYGHMTRFAPGSKPGA
                     RVKQGQIIGFVGSTGRSTGPHLHYEVLVNNKFVNPMTIAVPRGLQLEGRELAAFQKER
                     RRVEALMELDPVTSRVAQVNTQ"
BASE COUNT         2353 a         3625 c         3924 g         1993 t
ORIGIN      
        1 cgcaggccgc ggagcgccac gccacgccgc tcacgctctg cggggaaatg gccggacggc
       61 cgctggaggc catggcgctg atcgggctcg gcttccggtc gatttccgta gcgccggcat
      121 ctgtggggcc catcaaggcg atggtcctgt cgctcgacgc gggccaattg gctgagcggc
      181 tggatgcgct actggcagga ggagagacct ccttacgcga ggacttgaag cgttttgccg
      241 cggaaaacgg cgtccaagca tgagtttttc tgcttcggac ggtggtcggc accggtccgc
      301 cgatatgcta atcttgccaa gacttgcaaa cacacggcca ttgggcttgt gttaagccaa
      361 ggatcgcaag aggggggtcg gccgagctct agttgctcgg ctgtagcggg tgccagtaaa
      421 cgcgtcgccg attatggggc ggcagcgtct tcgtattcac gggatttatg agtatgccag
      481 ccggctatga tgcggaagtg gccgatctgt ttcaggattt cagggttgcc ataagtaaaa
      541 atactggcag caacctgacc gaagcagacc tcgctgcacg gatcgccacc cccgtcgagg
      601 tggttcaggc gcttgaacag ggcgcgattt atgccttgcc gccttggccg gagacggccc
      661 gcgtggtcaa cgcctatggc tcgcttctca acctcgacac gcggccgctg ctaaggcgca
      721 tctacgctca gatcgaagca ggcgtcgtgg agttgggacc gaagcccgtg ccggacgtcc
      781 ccgtcatgcc gccacaagcg ggcggggatg cggctcttgg cggagcggct cccgctcagc
      841 cacaacgcca gcctcaacct cagccacaag ggatgccgca agcagcgccg ggccaaccgg
      901 gctggcagaa cggcccggcc caacctcaac cgcaagcgcc gcatccccaa acgccgttgg
      961 cgccgaattg gcccggcggt gcaccgcaag cgccgcatgc cggcatgcct ggcgcccctg
     1021 ggcagccacc gcaacaagcc ccggcggctc ccgcttggtc gaacggccct caaggcgtac
     1081 cgcagccaca agtgcccaat cccgccgcct accagcagcc gccacaagcc tatccgccgg
     1141 agggtgcgct gccgcttcag ggcgaggatg cgagtttcga ggacgccgcc atgggtggcg
     1201 acgcggccat gggtgacgct gggaggggtg acgcggcgat gggcagcgat gcggccatga
     1261 tggacccaaa cctgggcgat ccagccatga gtgatccagc gatgggcgac ccagccatgg
     1321 gtggcgcaga gatgggcgat ccggcagctt ttggtgccga accggagcca aagaagaaaa
     1381 ggccggcact cctcaaatgg ggcatcgccg ctctggttgc gtcggtggtc ctcgttggcc
     1441 tatggatgtt ccttggcgat tcctctgact cctcgtcgtc ttcagggtcc tttgcctccg
     1501 atcccgtgct cgacccggac gatccgcgca gccgcaaggc cgatcgcctt ccgagcaagt
     1561 tctagacgtc ccatttctgc tccatgacca cgattcctgc cgagaagctc gacaagctcg
     1621 tcgcccgttg ggaggccgtg caaggcagtc ttgcctccgg cgccgatcag gagagctatg
     1681 tccagctgtc ccgcgaattc gccgagctcg atcccatcgt cgccacgatc aacgcgttgc
     1741 gtgcggcgtt agctgagcgc gagggcttgc aggagattct cgacgaccgc gcctcggacc
     1801 cggacatgac ggcgctcgcc gaggacgaaa tcggcgggct cgccgagaag atcgagacgc
     1861 tcgagcatga acttaaggtg cagctcctgc ccaaggacgc cgccgacgac aagagcgcga
     1921 tcctggaagt gcgtgccgga accggcggcg acgaagcggc actattcgcc ggcgatctgt
     1981 tccgcatgta tcagcgctat gcggaccggc atggatggaa ggtcaacatc atgtcggcga
     2041 gcgagtcggc gacgggtggc tataaagaga tcatcgccaa tatcactggc aagggcgtgt
     2101 tcgcgcggat gaagtacgaa tccggtgtgc atcgggtgca acgcgtgccg gaaacggagg
     2161 cgcaagggcg cattcatacg tcagctgcga ccgtggccgt gttgcccgaa gccgaggacg
     2221 tcgacgtgca gatcgacgag aaggatttgc gcatcgatgt gttccgggcg agtggccccg
     2281 gcgggcagtc ggtcaacacg acggacagcg cggtcaggat cacgcacctt cccaccggtc
     2341 tcattgtcac ccagcaggac gagaagtctc aacacaagaa caaagccaag gcgatgaagg
     2401 ttctgcgtgc gcgcctctac gatgccgagc gcgagaagct tgacgcggag cgggcgcagg
     2461 accggcgcag tcagatcggc tccggcgacc gatctcagcg catccgcacc tataacttcc
     2521 cgcaaggccg ggtgaccgac caccgcatcg ggctgaccac gcatcagctc cagttggtgc
     2581 tgcaaggcga gcctgcgctc gacgaagtcg tcgatgcgct gatcaccgag gatcagacca
     2641 gccagctcgc cgccatggag caagagtcgt gatggtatcg atcgatgcgg tccgtaacga
     2701 aacggcccgt acattgcgcg cggcgggtat cgacagccct gcgctcgatg cgcggcttct
     2761 tctttgcgaa gctgtgggcg tgacccatga ggcgctggtg gcgcgcgggc gcgaggacct
     2821 tccgccgcat gccgcggagc ggctgtccgg atatctcgcc cggcgtcttg gcggcgagcc
     2881 cgtgtcgcgg ataaaaggca aacgcgaatt ctacggccgc gactttttga tcgatacgca
     2941 cacgctcgat ccgcgccctg ataccgagac gcttattacc gcggcgctcg atctcgtggc
     3001 gcgcgagggc cggaccggga gcccgctcag cgtgctcgat ctcgggaccg gctcgggatg
     3061 catcttgctg acgctgctgg ccgagttgcc ggaggctcaa ggcctcggca cggatatcgc
     3121 gccgggcgcg cttgaggttg ccgcccggaa cgcaagctgt cttgggcttg aactccgcgc
     3181 gcggttcctg ggcactagct ggctcgaggg gctcaagggc ggcttcgatc tcatcgtgtc
     3241 caacccgcct tatatcgcgc gaggcgagat tggagggctg gcccgcgagg tggcggtcca
     3301 cgaccctatg gcggcgctcg acggcggcgc ggacggcctc gatgcctacc ggaccatagc
     3361 ggccagagtc ggggatttcc tgagccctgg cgggcacctg ctggtggaaa tcggggccgc
     3421 gcaagccgag ccggttagtc ggattttcca tcactcgggg cttggtattg atagggaggg
     3481 catattgtcc gatctggccg ggcgcccccg ctgcgtcctt gccaggagcc cgttttgagg
     3541 ggtctccgaa gggccggaat gcccaaaaat aagcttggaa aacccagatg ttcgggctag
     3601 tttcgaacca gcgaaatgaa tgtgcggcct gcgtgagcgg atattttagc gcggccggtt
     3661 catggcaact gtagcggata actcgtgaac ctcgattgac aggccttcgc ctctcgggtt
     3721 gttcaagagt cgctcaatcg cccatcagat tcggttggct ggcgctggcc aagaggtgca
     3781 agacgcctag cgctgtgagc cagcccaaga caaggcagca ataaagaacc gttgtatcga
     3841 ctgtggccga attggcgttc caggcgcttg ccgggagctt tggcggcagt cttgggagct
     3901 agtagggaag gacgcggcta gagccgccaa tcaggtgaaa taggcttatg aggcaaggac
     3961 agcagaatcg caggggccgc ggccgtggcc gcaagcccca gaacccactg tcacggaact
     4021 acgagtcgaa cggacccgat gtgaaaatcc gcgggaccgc gtcacacatc gccgagaaat
     4081 acatgtcact tgcgcgcgat gcgctcgcgt ccggcgacat ggtcaccgcc gagagctacc
     4141 tccagcacgc ggagcactat aaccgcatca tcatggcggc tcagccgcag atgacccctc
     4201 agaccgggga ccagcccaat ggcggcggtc ggcagcgcgc caaccctgag acgtccgaag
     4261 ggggaggcgc cgagcagcag caacagcagc aacagccgcc gcagcccgag acggcccaag
     4321 ccacggacgc tgccagccca gcccgttcgg aagaacgtgg ccgtggccgt ggccgcgggg
     4381 gcaacggcgc cgagggcgtc caggatgcca atggtgcgga gccagtcgag accgaggctg
     4441 gcggcgaaga caagagtgcg ccagacccgg tgagctaagc tcctctttcc caagatttct
     4501 tcagacgaac gtttggacgg tgatgcagcc gagccgccgg gccgcttgcg ctttgtgctg
     4561 cctcaaggcg cgctcccctt ttttgcggac gctggcgcgc tatatgtccg ttaacatgcc
     4621 gctggtatga cgctcgacca acggttcccg ccaccgaccc atagttccag gcacaccctc
     4681 acacctcgcc gctccgaaga gggacgatca tggattttga aaagctttcc gaccgtctga
     4741 agggattttt gcaggctgcg cagaccattg cactacgcga tggcaatccg cagatcacgc
     4801 ccgagcattt gctcaaggcg ctgctcgacg acgaagaagg tatggccgcc ggcctgatcg
     4861 ctcgcgcagg cggcgatccc aagacggcgc tggcccgcac cgatgccgcg ctcgccaagc
     4921 tgcccaaagt gtccggctcc ggcgccaccc agccgcagat gacgccgggc ttggcgcgcg
     4981 tgctcgacca ggccgagaag attgccacca aggcgggcga cagtttcatc accgtggaac
     5041 gtgcgctcca ggcgctcgcc atgaccaagg aaggcgagac cgccgatatt ctcaaagatg
     5101 ccggggtcac gccgcaaagc ctgaacgaga ccatcaacga tctgcgcaag gggcgcaccg
     5161 ctgacagcgc cggcgccgag cagcaatacg acgcgctcaa gaaatacacg cgagatttca
     5221 ccgaggccgc cgaaaagggt gagatggacc cggtgatcgg ccgcgacgag gagatccgcc
     5281 gcaccatcca agtgctgtcg cggcgcacga agaacaaccc cgtgctgatt ggcgaacccg
     5341 gcgttggcaa gaccgccatc gtcgaaggcc tggcgcagcg catcgtcaaa ggcgacgtgc
     5401 cggacagcct gaaggacaag aaggtgctgt cgctcgacat gggcgcgctg atcgctggcg
     5461 ccaaatatcg cggcgagttt gaggagcgcc tcaaggccgt gctgtccgag atcgaagcgt
     5521 cggaaggaca gatcgtcctc tttatcgacg agatgcatac gctggtcggc gccggcaaag
     5581 ccgacggcgc catggacgcc tccaatctgc tgaaaccggc attggcgcgc ggtcaactgc
     5641 attgcgtcgg cgccaccacg ctcgatgagt accgcaaaca tgtagagaag gacgcggcgc
     5701 tcgcccggcg cttccagccc gtcttcgtgc cggagcccac ggtggaggac accatctcca
     5761 ttctgcgcgg cttgaaggaa aagtatgagc tgcatcacgg cgtacgtatc agtgatgccg
     5821 cgctggtcac cgcggcgacc ttatcgaacc gctacatcac agaccgcttt ctccccgaca
     5881 aggccatcga cctcgtggat gaggcggcca gccgcctgcg catgcaggtg gactccaaac
     5941 ccgaggagct cgacgagctc gatcggcgtc tcatgcagct aaagatcgag cgcgaggccc
     6001 tgaagaagga gaccgacaac gcctccaagg accgtctcga aaagctcgag aaggaaatcg
     6061 tcaatctgga agagagcgcc aatgtgcttt cccagcgctg gcaggaagag aaagacaagc
     6121 tcggggcggc gcagaagatc aaggaagacc tcgaccatgc gcgcatcgag ctggacaagg
     6181 cgcagcgcga cggcgacctg accaaggcgg gcgagctggc ctatggcgtg atccccgatt
     6241 tggaagcgaa gcttgccaac atcgaagagg aagagggcaa ggccgggagc ctcgcccaag
     6301 aagtcgtcga gcccgacatg atcgcagctg tcgtgtcgcg ctggaccggc attcccgtcg
     6361 acaagatgct cgaaggcgag cgcgagaagc tgcttcagat ggagaccgag atcgccaagc
     6421 gcgtggtcgg ccagaaggag gcggtgcaag ccgtgtccac tgccgtgcgc cgcagccgcg
     6481 ccggcctgca agacccgaac cggccactcg gctcgttcat gttcttaggc cccacgggcg
     6541 tcggcaagac ggaactctcc cgcgcgctcg ccgagtttct attcgatgac gagcacgcga
     6601 tcctgcgcgt cgacatgtcc gaatatatgg agaagcacgc ggtggcccgg ctcatcggcg
     6661 cgcctcccgg ctatgtcggt tacgaagaag gcggcgcgct caccgaagcc gtgcgccggc
     6721 gcccctacca ggtcattctc ttcgacgaga tcgagaaggc gcatccggac gtgttcaacg
     6781 tcttgctgca agtgctcgac gacggccgct tgacggacgg ccagggccgc acggtcgact
     6841 tccgcaacac gctgatcatc atgacgtcca atctgggctc ggagttcctc gtcaaccaga
     6901 aggaaggcga ggactcagat gccgtggagg accaggtgat ggaggtggtg cgcgggcatt
     6961 tccggcccga attcctcaac cgcgtggacg agatcatcct gttccatcgt ctagccagag
     7021 aagacatggg ccagatcgtc gacatccaaa tgaagcggct gctcaagctc cttgccgacc
     7081 gcaagatcac gctgacgatc gacgacgacg ccaagacttg gctcggcaac aagggctacg
     7141 acccggccta cggggcgaga cccttaaagc gcgtgatcca gaaatacgtg caagacccgc
     7201 tggccgagca gatcctggaa ggcaagatcc gcgacggcgc gaccgtcaag gtcggcgtca
     7261 aggacggcgg gctgacgttt aacggtgagg cggcggacgc gacggggggt taggggttgg
     7321 gggagcgttg gtacgcttgc tcgtaccgcg caacggagcc taaacagcct tctcggcgcc
     7381 gaggcccgta ttggcgcgca cggagaactc ggcgaacttt tcttccgccg ctctttcgcg
     7441 agtgagcagg gtcgccacga tggcggcggc aaagccaagc ggtacggaga cgatgcctgg
     7501 attgcgcaag gggaagatcg gctcgccggc gatcagcgtc gtgccgtccg tgcccatgat
     7561 gcttgggctg agcaggatta gcgcgatcga gctgaccagc ccgacgccaa gtccccaaca
     7621 cgcgccggcc gtatcaaaac gcttccagaa gagagcgagc accatcggcg gcaggttggc
     7681 acttgccgcc acggcaaacg cgagcccaac gagaaaggcg acattggtcg agcggaggcc
     7741 gatggcgagc agaatagctg ttgcgcccac gacgaacgcg gcgatgcgcg ccacgcggac
     7801 ctcttcgccg ggcgcgcgtt cgcggccggc atgaatcacg ttcgtgtaga aatcatgcgc
     7861 gaagctggtg ctggcactga tggtgagccc tgcgacgacg gcaaggatcg tggcgaaggc
     7921 gacagcgcta atgaaggcaa agaagatgtc gccgccgaga tgctgggcaa ggagcggcgc
     7981 cgccatgttt tgatcgggaa tgttcgcagg cgtcagcagc gtggcggcgc caaaaccgaa
     8041 gaacgtcgtc atcaggtaga acgcgccgat cgcgaccatc gcccagacca cgctgagccg
     8101 cgcggttctc gcgtcgggca cggtgtaaaa gcgaatgagg atgtgcggca ggccggccgt
     8161 ccccaagaca agcgcgatgc cgagcgaaat cagatcgata gcgcccagcg tgcctttgaa
     8221 ggcaaggccc ggctgcagga agttctgcgt gacttgcgcg cccgtcttcg ggtcgatcca
     8281 ggtgacgtga ctgaccgctt caaagaagct accgaagctg tagccgaact gcgccatcac
     8341 cagaatgctc agcatgacgg atccggccag gagcaaaatg gccttggtga tctggaccca
     8401 ggtcgtggcc agcatgccgc cgaacacgac atagatgatc atgagcacgc cgacgccaat
     8461 cacggcgagc tcgtagctca cgcccggcag cagcagcttg acgaggctgc cggcgccgac
     8521 catttgcgcg atcatgtaga agatggtcac ggtcagcgtg ctgagcgcgg ccatggcgcg
     8581 caccggccgc gccttcaagc gataggccag cacgtccgcc atggtgaact tgcccgagtt
     8641 ccgcaacggc tcggcaacga tcaacagaac cgtgaggtag gcgaccagaa agccgaccga
     8701 gtacataaag ccgtcatagc ctttcagcgc gatcatgccg gcgatgccga gaaagctcgc
     8761 cgcgctcata tagtcgccgg cgattgccaa cccgttctgc caggctttga tgcggcgtcc
     8821 ggccgcaaag taggcgctcg ccccgccaga gcgccgcgcc gaccaccacg tgattagaag
     8881 ggtgaacgcg acaaagagca gaaaaagcca aatcgccgtg gtcatgtggt cttgcttccc
     8941 gaactgacaa gagcttcgcg gcggatcttc gcggcgagcg catcaaaccg ctttgcgtgg
     9001 cgcaaatagg cgtaggcgac gaggccgacc atggcgaatt gggagagcgc gaagagatag
     9061 gcgaggttta ccggcccaag ccaggacacc gtccgcgaca tcacttccgg aaagaaaccg
     9121 accagcacaa ggagggtaaa gtagtaggcg aggaagaaga tggtcgccgg gatgagaaat
     9181 cgtgccctca agtcgactag cgtcttgaag tcgttcgttg aggccacgcg gtcccaatcg
     9241 accacgtcga tgtcttcgtc cgccgtttgc tcgtgcggtg gcggggcctc ggtccggccg
     9301 acgcgcgtga catctgtttc ggcgctgacg gaggtcgtca ggtccaaatc ttttttgctc
     9361 aacccttaat ccctcccggt ccgcgtagac gcgctatccg cgaatcttcg tcgggcgctc
     9421 cacagggcct tccaacattt tcagcagcat aacaagattt tccggaggtg cgccgcgacc
     9481 ccggacggaa atgctgcttc gcgcccagag tggacgcagt cccctttctg ataaaggtca
     9541 ggagaacaag gaaaaaggag ccgcagctcc agcaaaatca cccgttcttc ttggcgcggt
     9601 ggcgatcaaa aaaaccaaag gcaagtgggc tcgatcccga agggttggga gggaaattgg
     9661 ccgaaaccaa gaaacccaat atcggtctag cgctgtccgg tggtggcttc cgcgccacgc
     9721 tgtttcatct tggccttatc cgctttctgc gcgatgcggg gctgctctcc aacgtcaccc
     9781 agccgccgaa cgccgttgca ttgggtactt tcgagcgcaa gccgcggcgg ctcttgggga
     9841 caaacgtatg gaggagttct gccacaagga gctctcccgc gcaccgtccc ccaccatcgt
     9901 gacttgaggt gcgctccgcc gcttgagcaa gatcaaggcg cgctgccgtg aaagccaggt
     9961 gaactgacgg gcaggcttgg agaagcgaag aagagcggtt tgccactgga ggggcggcat
    10021 gaatgttctg atcgcctacg gcacgactga ggggcaaacg cggaagattg ccgagacggc
    10081 ggcggcgcat ctgcgcggcg ccggtcatga ggtcgcgctc tatgataccg ccgatcccgg
    10141 tccccctgta gagctcgacg gattcgacgc gtccgttatc gccgcgtctg ttcatcagga
    10201 gcagcaccag cctgtggtga agcgcttcgc ggccgcgcat tgccaaagcc tgaactcgat
    10261 gccttccgca tttttatcag tcagcctgtc ggcggcgttg gaggaaacgc gtggcgaagc
    10321 gcaagcctat gtagaccgtt tcctcgcggt gacgggatgg cagccggcca tgacgctcct
    10381 cctcggaggc gctctgcggt tcaccgagta cgacttcttc caagagcaga tcgtcaagtt
    10441 cattgtcatg aagcgcgggg tcgtttcgga gacggagtcc gaccgcgagt tcacgaactg
    10501 ggacgctttg tccagctttc tcgatgcgtt tctaatgacg gccgaggctc gttgacggga
    10561 gctatcgcgc gcgcggatgc tgcggctggc ttgggaatcg caatgtaaag ggtagtgaag
    10621 aggcggggac ggtgggctga cgtccaggct tactgggtgt tgacctgggc gacgcggctg
    10681 gtgacgggat cgagctccat gagagcttcg acgcggcggc gctctttctg gaaggcggcg
    10741 agttcgcgtc cttcgagctg caggccgcgc ggcaccgcga tggtcatcgg gttgacgaac
    10801 ttgttgttga ccaggacttc gtagtgaaga tgcgggccgg tggagcggcc ggtcgagccg
    10861 acaaaaccga tgatctggcc ttgcttgacg cgggcgccgg gcttggagcc gggggcgaag
    10921 cgcgtcatgt ggccgtaggc ggtggcgaat ccgttggcgt ggcggatgcg gatatagttg
    10981 ccataaccgc cgtgacgctc tgccatctcg atcgtgccgt cgccggcggc gaggatcggg
    11041 gtgcctgagg gcgcggccca atcgacgccg gtatgcatgc ggatgcggcg cagcagcgga
    11101 tggcggcgat tgccgaagcc ggaggtgaag cggccgccct tgaccggatt gcgcatcaag
    11161 aacttcttcg cgctcgagcc gttctcgtcg tagaagtcga tcgtgttgtc cggcgtgcgg
    11221 aagcggtaga acttgcgggt ctcgccgccc acggtcattg aggtgtaaag caactcgccc
    11281 gcgtcgctgc cgctcacgcc ttcgccgtcg aagaacatct cgaatgtgtc gccggagcgg
    11341 accttctgct tgaagtccac atcataggag tggacgcgca gtagcttgac gatcgtatcg
    11401 gcgggtagct tctgctccag cgcggcggtg tagaagctct catagagcga ggcgcggtcg
    11461 gaggcggtga tgacgacctc gtccacgggc gccgtggtgg aggcgagata gtcgccgtcg
    11521 cgattgcgcg tcgcggaggc gatatggttg cccttgcgat cgaacacgct gacgcggacg
    11581 acgtccttag cctcgggatt ggcggcggac ggcaccagcc ggaagtccat cttctggcgc
    11641 ggcttcaggt cctcgggcgc gaaaacggca tcgaggctgt cgatgatggc gctcgccatc
    11701 tccttgtcgg tgccggattt gaggagcacg cccatgagat tgtcgccgct gccgaccgtg
    11761 atgaccttag tttcggcgcg ggcgatgtcc ttctcggtcg acgcttcgtc ctcgtccagg
    11821 accttccgca acacagtggt gtttggcata agccggggac gctcgaactc agcgggacgg
    11881 tgggaggcaa ggtgc
//
LOCUS       DBNHVU010000125         2529 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_865299, whole genome shotgun sequence.
ACCESSION   DBNHVU010000125 DBNHVU010000000
VERSION     DBNHVU010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_865299"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            647..919
                     /locus_tag="AC16Y7_04950"
     CDS             647..919
                     /locus_tag="AC16Y7_04950"
                     /inference="COORDINATES: protein motif:HMM:NF023174.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 domain-containing protein"
                     /protein_id="HIR5708760.1"
                     /translation="MLFVCYVTIDPDNRDESVARFKEYGVAEPEGVKLQGAWISSTQQ
                     ECWSVFEADSAGAIMAMFEPWTDLNMHQITPVMDFDALKKFVDAND"
     gene            complement(925..1383)
                     /locus_tag="AC16Y7_04955"
     CDS             complement(925..1383)
                     /locus_tag="AC16Y7_04955"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HIR5708761.1"
                     /translation="MRRQTSGDLPGAVLFACSRNAVRSPMAASLLMHLAGNRIYVESA
                     GVRPGVPDPFAIAVMEEMGIDMSGHVPVSIDDLHDLSFDLIVTLAPEAQHKALELAEG
                     YAIDVEYWPTPDPTLASGAREQILDAYREVRDRLFAKIRERFPLPSAPGV"
     gene            complement(1380..1883)
                     /locus_tag="AC16Y7_04960"
     CDS             complement(1380..1883)
                     /locus_tag="AC16Y7_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HIR5708762.1"
                     /translation="MGDEAEEQTWSEGARLIEVTLDEDSLGRDNVEVEHERKVAIFDL
                     LEKNNFALESHETSGPYNLRLSLADNRLVFSVSDADSAPLQHVMLSLSPFRRIVKDYF
                     LICDSYYEAIKTQPASKIEAIDMGRRGLHDEGSHLLMERLKGKIAVDIATARRLFTLL
                     CALHWKG"
     gene            complement(1900..>2529)
                     /gene="hisD"
                     /locus_tag="AC16Y7_04965"
     CDS             complement(1900..>2529)
                     /gene="hisD"
                     /locus_tag="AC16Y7_04965"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018094177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR5708763.1"
                     /translation="VFGQVGIDMVAGPSEVVVIADADNDPAWIAADLLAQAEHDTAAQ
                     SILITDKEDFAAAVVEAVEVQLKTLPRGDTARASWDEFGAMILVRDLNEAPAIADRLA
                     PEHLEIATENADALAAQVRDAGAIFVGRYTPEVIGDYVAGTNHVLPTARTARFASGLG
                     VLDFMKRTSLLKLDAASLKALGPAAMTLARAEGLDAHRRSVAIRLNLPD"
BASE COUNT          464 a          827 c          800 g          438 t
ORIGIN      
        1 tcttccttcg ccataaggct ccttccgcat cgtccggttg ccgcggagaa tggtcgggcg
       61 ggcggccaaa atcaaggacg attcccgtgt ttttgcgctt ctggccaagc caaggcgtcc
      121 cgccgcgaaa cccccgcgcg tggcttgagc catgaggaca gcggggcacc cgtcggccgc
      181 gtcctgctgc agcgtcttgg gcggccttcc ggcgccccgc ttcaggcgat atcaagcccc
      241 cttccgcacg gcttaccacc agtggcacag gcgcaaaggg ctcaagcgcc cggcgcacct
      301 cacccgacga cccacgcgtt acagcgcagg tgggcggagc ggggaccccc gaggctggtc
      361 cgttgtccat gacgcggtgg tccgggcgcg gcgcttggca atcgggccca tctgtcccgc
      421 gccacctcgg gcgccacacc cctcatcctc aagaagcaga cggtctgcag ctcgcgtcct
      481 cacggacgag ggacggagac agtgtaaggg cctttggcgg ggcggggata agattgcgcc
      541 gcccccgctc cccggggaac tatcgtgggc cgcagcggct tcaaaaagat gtcgcagaag
      601 cggctatata tgtggcgccg cgcgaccatc ctgaggagaa cgagacatgc tgttcgtctg
      661 ttacgtgacc atcgatcccg acaaccgcga cgagagcgtc gcgcgcttca aggaatatgg
      721 cgtggccgag cccgaagggg tgaagctgca gggcgcctgg atctcctcca cccaacaaga
      781 gtgctggagc gtctttgagg ccgacagcgc cggggcgatc atggcgatgt tcgagccctg
      841 gacggaccta aacatgcacc agatcacccc ggtgatggat ttcgacgcgc tcaagaaatt
      901 cgtcgacgcc aacgactgag cgccctagac gcccggcgcg ctgggcagcg ggaagcgctc
      961 tctgattttg gcgaacagcc ggtcgcgcac ctcacgatag gcgtcgagga tttgctcgcg
     1021 ggcgcccgac gccaatgtcg ggtcgggcgt cggccaatac tccacgtcga tggcgtagcc
     1081 ttcggccaac tccagcgcct tgtgctgcgc ttccggcgcg agcgtcacga tcaggtcgaa
     1141 gctgagatcg tgcagatcat cgatcgacac cggcacgtgg cccgacatgt cgatgcccat
     1201 ctcctccatc acggcgatgg cgaagggatc ggggacgccg gggcggacac cggcggactc
     1261 cacatagatg cggttgccgg caagatgcat gagaaggctt gccgccatgg gcgagcgaac
     1321 ggcgttgcgg ctacaggcga agaggaccgc gcccggcagg tcgccgcttg tctgcctcct
     1381 caccccttcc aatgaagcgc gcagagcagg gtgaacaagc gcctggccgt ggcgatatca
     1441 acggcgatct ttcctttgag gcgctccatc aagagatggc tgccttcgtc gtgcagtccg
     1501 cgccggccca tgtcgatggc ttcgatcttg gaggcaggct gggtcttgat cgcttcgtaa
     1561 taactgtcgc agataagaaa gtagtctttg acgatgcggc ggaagggcga cagcgacagc
     1621 atgacatgct gaagcggcgc gctgtccgca tcgctgaccg agaaaacaag acgattgtcg
     1681 gcgagcgaaa gacgcaagtt atacggccca ctcgtctcat ggctctccaa ggcgaagttg
     1741 ttcttctcca ggaggtcgaa gatcgccacc ttgcgctcgt gctcgacttc gacattgtcg
     1801 cggccgagcg agtcctcatc gagggtcacc tcgatgagcc gcgcgccttc cgaccatgtc
     1861 tgctcttctg cctcgtcgcc catgtctagt tgcccatgtc tagtccggca ggttcaagcg
     1921 aatggcgacg gagcggcgat gcgcatccag cccttcggcg cgtgcgagcg tcatggccgc
     1981 agggcccaag gcctttaggc ttgccgcgtc gagcttcaac agcgaggtgc gcttcataaa
     2041 gtcgagcacg ccgagccccg aggcgaaacg cgccgtgcgg gccgtcggca gcacgtgatt
     2101 ggtgccggcc acgtagtcgc cgatcacctc gggcgtatag cggcccacaa agatggcgcc
     2161 cgcgtccctc acctgcgcag caagcgcatc ggcattttcg gtggctatct ccaaatgctc
     2221 gggcgccaac cggtcagcga tggcaggcgc ctcgttcaaa tcgcgcacca aaatcatcgc
     2281 gccgaactca tcccagcttg cacgcgcggt atcgccacgc ggcagcgttt tgagctgcac
     2341 ctccacggct tccaccacgg cggcggcaaa gtcctctttg tcggtgatga ggatcgactg
     2401 cgccgcggtg tcgtgttcgg cctgcgcaag caagtccgcg gcaatccacg ccggatcgtt
     2461 gtccgcatcg gcaatgacca caacctcgga cggcccggcc accatgtcga taccgacctg
     2521 gccgaacac
//
LOCUS       DBNHVU010000126        11443 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_939049, whole genome shotgun sequence.
ACCESSION   DBNHVU010000126 DBNHVU010000000
VERSION     DBNHVU010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 11443)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11443)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11443
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_939049"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="AC16Y7_04970"
     CDS             <1..336
                     /locus_tag="AC16Y7_04970"
                     /inference="COORDINATES: protein motif:HMM:NF019998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HIR5708764.1"
                     /translation="RSTVRGGRAETPLGTFPAKGLAEGTDAIVCVRQSGVRLLKPGKG
                     TPGRILDTRFLGDVGLVEVAVQGLDDPVLARVREADVPEKGAEVGVAVDEGAILVFEA
                     GNGDEGPSP"
     gene            432..656
                     /locus_tag="AC16Y7_04975"
     CDS             432..656
                     /locus_tag="AC16Y7_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIR5708765.1"
                     /translation="MGPSWWQILIVLVLFVLLFGRGKISDLMGDVAKGIKSFKSGLAD
                     DDEKADAKVIDHETPAPKSRARAPHKKKSS"
     gene            737..1222
                     /gene="tatB"
                     /locus_tag="AC16Y7_04980"
     CDS             737..1222
                     /gene="tatB"
                     /locus_tag="AC16Y7_04980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - transmembrane protein
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HIR5708766.1"
                     /translation="MLNIGWSELLLVAIVAIVVVGPKDLPKLMRTFGVYAGKLRRAAA
                     DFQRQFDEALAESEADEVRKNIEAIRSNMGTMADFAPPPDTPLMTPTTPAAQSIEHAI
                     APRAKTARTKKASAKKTAAKKTPRKTAAPKRKASPKRKAASPKRKTADKRTTTKTRRK
                     R"
     gene            1219..2007
                     /gene="tatC"
                     /locus_tag="AC16Y7_04985"
     CDS             1219..2007
                     /gene="tatC"
                     /locus_tag="AC16Y7_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR5708767.1"
                     /translation="MSERDDIDATKAPLIEHLVELRRRLMWSLAAIFAAFVVCFWFAK
                     PIYNLLLWPYRVAAGVDVPIELIYTAPQEFFFTQVKLALFGAIFVAFPVIATQLYIFV
                     APGLYRAERKAFLPFLIATPILFLIGAALVYFVAMPLAMTFFLSMQQTGDVEILLTAK
                     VSEYLSLIMMLILGFGICFQLPVLLTLLARAGLVTSDQLKYYRRHAILGVFVAAAILT
                     PPDPVSQLALAIPTLGLYELSIFAVKLVEKKRAASGAAAASPSS"
     gene            2117..3409
                     /gene="serS"
                     /locus_tag="AC16Y7_04990"
     CDS             2117..3409
                     /gene="serS"
                     /locus_tag="AC16Y7_04990"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR5708768.1"
                     /translation="MLDVKWIRENPEAFVKGLTDRGFESPQGTLNHILKLDEERRETI
                     QTLEEAQASANTASKAIGKAKQAKDEAEAASLLEKVAGLKDIIQDRESAKRLLDGSLR
                     ELLAGIPNMPADDVPVGEDERENVELRKVGEPPAFDFTPKQHFEIGEELGLMDFETAA
                     KLSGARFVVLKGPLARLERALAQFMLDLHTSEHGYTEVNPPLLVRDDAMFGTAQLPKF
                     REDQFAAGDDYWLIPTAEVPLTNLARDDIADEAKLPMRVTAGTYCFRAEAGAAGKDTR
                     GMIRQHQFYKVEMVSVTTPEASAEEHERMTACAETVLKRLGLPYRVVVLSTGDMGFGA
                     RKTYDIEVWLPGQGMYREISSCSNCGDFQARRMNARYRPKDRKATEFVHTLNGSGVAV
                     GRALIAVLENYQEEDGSVRVPAALQPYMGGLTRIEAPK"
     gene            3605..4378
                     /gene="surE"
                     /locus_tag="AC16Y7_04995"
     CDS             3605..4378
                     /gene="surE"
                     /locus_tag="AC16Y7_04995"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR5708769.1"
                     /translation="MRVLITNDDGIEAAGLDVLEKIATDLTDDVWVVAPDTDNSGASH
                     SLTLAEPLRMRELAKRRFAVKGTPTDCVIMGVRYLLKDNPPDLVLSGVNRGQNLADDV
                     NYSGTVAGAIEGSLLGVPSIALSLAIGSYDTGVPAWDTPMVHGAGLVRKLLDAGWPKS
                     TVLNVNFPDCEPNQVKGMAATVQGLRDTALLDISARMDVRGKAYYWIGIAKRGSNPPK
                     DTDLSAIRANLISVTPLCLDFTDRQTLGALADVLGEKDK"
     gene            4461..5102
                     /locus_tag="AC16Y7_05000"
     CDS             4461..5102
                     /locus_tag="AC16Y7_05000"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR5708770.1"
                     /translation="MASAQDQIGLIMQLRRRGIRDPKVLRAMEAVPRETFVDEAFTEH
                     AYQDIALPIDCGQTISQPFVVAYMTEKLGLEPTHKVLEVGTGSGYQAAVLSHLCRRVY
                     TVERWRELQKVAEARLADLKIANVTTIIGDGWLGWPPQAPFDRIIVTAAALDAPAALL
                     DQLKDGGRMIIPLGENRDSQYLVQIDKTEEGMVETPLLPVRFVPLVHGRVKRD"
     gene            5201..6301
                     /locus_tag="AC16Y7_05005"
     CDS             5201..6301
                     /locus_tag="AC16Y7_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR5708771.1"
                     /translation="MRGAFCLEFGRGTVRVVAAVGLAFAMAGCSSSARFDDSPYSLSD
                     SDALADPSTTASLPVPSQSVYNGSSGSGQIARSTPPPQSTAAYDTSKPRYYGTPRSAK
                     PPVPAPMTQASGPRIKVKQGDTLSGLSQRYGVPIDTIQSANNLADGRLREGQVLIMPV
                     ASPQAPAPAVAQAPAPAPAPASSGARKVKTTTIQAPGTPAPTETTQSATSASKVASKT
                     PQNVTSTAQLPNPEPMTGNSFRWPVKGRVISGFGTKPDGGHNDGINISVPQGTPVKAA
                     ENGVVAYAGSELKGYGNLVLVRHANNWVSAYANNETLLVKRGDKVKRGQVIGKAGTTG
                     SVSKPQVHFELRKGSRPVDPTKYMSSMAAQAN"
     gene            complement(6298..7149)
                     /locus_tag="AC16Y7_05010"
     CDS             complement(6298..7149)
                     /locus_tag="AC16Y7_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5708772.1"
                     /translation="MNFQEPISGFMERIAHALERLGPARPQTPDFAHAGAFVFQAESE
                     RFAPVSSVNRVPLELLKGIDRNRNALLANTDRFARGLPANNALLWGSRGMGKSSLIKA
                     AHGAFPGLKLVEIHREDIAALPRLLTYLRAAPQYRFILFCDDLSFEAVDTSYKSLKAV
                     LEGGIEGRPDNVLLYATSNRRHLMPREMVENERSTAINPSEAVEEKLSLSDRFGLWIG
                     FHNCSQDEYLAMVRSYADHYGLDVPDAHLTQEALEWARGRGSRSGRVAWQFIQDLAGR
                     LGKQLDG"
     gene            7286..7630
                     /gene="yajC"
                     /locus_tag="AC16Y7_05015"
     CDS             7286..7630
                     /gene="yajC"
                     /locus_tag="AC16Y7_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR5708773.1"
                     /translation="MDGFAYAQASGGAFEMLNSLLVPTILIIGIMYFLMIRPQQKRMK
                     EHREMIASIRRGDTVVTSGGILGKVTKVEDNELQLEISDGVRIKVLRPTVSEVRGKDE
                     AGGASSSKSEAK"
     gene            7672..9285
                     /gene="secD"
                     /locus_tag="AC16Y7_05020"
     CDS             7672..9285
                     /gene="secD"
                     /locus_tag="AC16Y7_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIR5708774.1"
                     /translation="MLHFQKWKLILVFGIVLAGFIFAIPNLFPSSSLQGLPSWFPHKQ
                     VNLGLDLQGGAHLLYRIDDKELVDDWLGTIRGDVREGLRSNRIGYTDLSQNVAGRSVS
                     VRIRKPDDMDKAYTELKKQSVPVGGDVFGGFAGNNLEVTRDADKITLTVTEVGLTDRI
                     NSAIQASIETIRRRIDAFGTTEPSIQREGRNRILVQVPGVTDVQRLKSLIGETGKLEF
                     KMVDSTVNAAEVEVSKKVPPGDELLYSSDDPPVPYVLKDRVLVSGENLVDAQPGFDSR
                     TGEPVVTFRFDAAGAKRFGRATQENVGLPFAILLDNKVISAPVIREPILGGTGQISGN
                     FTVQEANDLSVLLRSGALPAKLTVIEERTVGASLGADSIESGKKAAIAGLLLVVLFMF
                     AAYGLFGFFANLALLVNIALIFAVLSLIGATLTLPGIAGIVLVIGIAVDANVLINERI
                     REEKRAGKSPIAAIDSGYSRALITIIDSNVTTLIAVLVLFWLGSGPIRGFAVTLTIGV
                     LASMFTAVTVTRMMIAYWLRWTRPKELPI"
     gene            9298..10230
                     /gene="secF"
                     /locus_tag="AC16Y7_05025"
     CDS             9298..10230
                     /gene="secF"
                     /locus_tag="AC16Y7_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR5708775.1"
                     /translation="MFRGITFIPADTKIDFVGQRAVTWFVSAFLTVVPLILVATIGLN
                     MGIDFQGGTLIEVKTKESPANLADIRGKVSGLGLGEVQIQEFGEPDEVLIRIASQPTE
                     EEQQESIARVKNALGDSVEYRRTEIVGPTISGELIAGGTLAVVVAMIGIMIYVWFRFE
                     WQFSVAAVASLVHDVTATIGLYALLQLEFNVSSIAAILTIIGYSLNDKVVIFDRIREN
                     LRKYKRMPLKNLLDLSINEMLSRAILTHVTTLLAMMPFLFFGGEAIYGFALAMCFGII
                     VGAYSSIFVASPMQLILGVQREAFSPRAAARANP"
     gene            10240..11091
                     /locus_tag="AC16Y7_05030"
     CDS             10240..11091
                     /locus_tag="AC16Y7_05030"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HIR5708776.1"
                     /translation="MRDDERRDVVRRIARDADPDRAIATLFAPAETRADLFALFAFNA
                     ELARIADQVSEPGLGAIRLQWWREAIERAASGDVTGNPVADAFGEVLARRTLSRERIA
                     GLIDARSFDVGETVMPDTRTLDAYLFDTTGGLFALAADIVGAGGEKRDLVVEAGARAY
                     GLVRVMRSLPVLAAKGRTYLASDALAHHGTSPQAIFAGETNDGLEVLLTEMREESRAA
                     LKEAQFHLFGEGMKGAGLDEAGRATFLPLCLVEPYLAALAKVGDPLRQTARINPLARL
                     WHLMRSR"
     gene            complement(11097..>11443)
                     /locus_tag="AC16Y7_05035"
     CDS             complement(11097..>11443)
                     /locus_tag="AC16Y7_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HIR5708777.1"
                     /translation="GYVESAAIGLIAGRFAAAERTAKPLLPPPPTTALGALLTHITGG
                     HLSADGEGAARSFQPMNVNFGLFPPVPAPPRPPGKAKRGAKSLARKQAMSARALADLA
                     AWLEGTATIAAE"
BASE COUNT         2137 a         3583 c         3632 g         2091 t
ORIGIN      
        1 cgcagcaccg tgcgcggagg ccgggccgaa acgccgctcg gcacgtttcc agccaaaggg
       61 ctggcggagg gaaccgacgc catcgtttgt gttcgccaaa gcggcgtgcg gctgcttaag
      121 cccggcaaag gtacgccagg acgcatcctc gacacccgct ttctcggcga cgtggggctg
      181 gttgaggtcg ccgtgcaggg gctcgacgat cccgtgctgg cgcgcgtccg ggaggcggac
      241 gtgcctgaga aaggcgccga ggtcggcgtt gccgtggatg aaggcgcgat cctcgtcttc
      301 gaagccggaa acggggacga ggggccttca ccttaaaggg agcccggctt gcgagcgggg
      361 cgcctttgct gccatagtga cggcaactct ttgataatgc gcgggggttc gcccctcgct
      421 ggaggccttt catgggaccg agttggtggc aaatcctgat cgtgctggtg ctgttcgtac
      481 tgctgttcgg gcgcggcaag atttccgatc tgatgggcga cgtagccaaa ggcatcaaga
      541 gcttcaagtc ggggcttgcc gatgacgacg agaaggccga tgccaaggtg atcgatcacg
      601 aaacgccagc gccgaagtcg cgcgcccgcg cgccgcacaa gaagaagtca agctgagctc
      661 tacaatgacg tggagtgtct ctcgcgcgcc ggacgttctt gcgcgcggca atcgttggct
      721 ctgacacctc gaacgcatgc tcaacatcgg atggtccgaa cttttgctgg tcgccattgt
      781 ggcgatcgtc gtggtgggtc ccaaggatct gccgaagctg atgcggacct tcggggtcta
      841 tgcgggcaag ctgcgccgcg cggccgccga cttccaacgc caattcgatg aggccttggc
      901 tgaaagcgag gccgacgagg tgcgcaagaa catcgaggcc attcgctcca acatgggcac
      961 gatggccgac tttgcgccgc cccccgacac tccgctgatg acgccgacga cgccggcggc
     1021 gcagtccatc gagcacgcca tcgcgccgcg cgcgaagacg gcacggacca agaaggcgtc
     1081 agccaagaaa acggcggcca agaagacgcc gcgtaaaacg gcagcgccca agcgcaaagc
     1141 gtcacccaag cgcaaagcgg cgtcacccaa gcgtaagaca gcggacaaga gaaccacgac
     1201 gaagacgagg cggaagcggt gagcgagcga gacgacatcg acgccaccaa ggcgccgctc
     1261 attgagcact tggtcgagct ccgtcggcgc ttgatgtggt cgctggcggc gatcttcgcc
     1321 gccttcgtgg tttgcttctg gttcgccaag ccgatctaca atcttctgct gtggccgtat
     1381 cgcgtcgccg cgggcgtcga tgtgcccatc gagctgatct ataccgcgcc gcaggagttc
     1441 ttcttcacgc aagtgaagct cgcgctgttc ggcgccattt ttgtcgcctt ccccgtcatc
     1501 gccacgcagc tctatatatt cgtagcgcca ggcctatacc gggccgagcg gaaggccttc
     1561 ctgccgttcc tcatcgccac gccgatcctg tttctaatcg gcgcagcgct cgtctatttc
     1621 gtggccatgc cgctcgccat gacgttcttc ctgtcgatgc agcagacggg cgatgtggaa
     1681 atcttgctga ccgccaaagt cagcgagtat ctgtcgctga tcatgatgtt gatcttgggc
     1741 ttcggcatct gcttccagtt gccggtgctg ctgaccctgc tggcccgcgc cggcctggtg
     1801 acgtccgatc agctgaaata ttaccgccgg cacgccattc tcggcgtgtt cgtggcagcg
     1861 gcaatcctca cgccgccgga tccggtcagt caactcgcct tggccatccc gacgctcggg
     1921 ctctatgaac tctccatttt cgccgtgaag ctcgtggaga agaagcgcgc cgcctctggc
     1981 gcggccgcag catctccgtc atcgtgaggc aggtccacgc cctgagacaa ggggaccgat
     2041 gacaagccca aggtgccgct ctatacgggg aaccacgcaa accgcccctt cgagtcgatg
     2101 aaagagattg ccccccgtgc ttgacgtcaa atggatacgt gagaacccgg aggccttcgt
     2161 caaaggcctg accgaccgtg gcttcgaaag tccgcaaggc acgctgaatc atatcctcaa
     2221 gctggacgag gaacgccgcg aaacgattca gactttggag gaggcgcaag ccagcgccaa
     2281 caccgcctcc aaggcgatcg gcaaggccaa gcaagccaag gacgaggcgg aagcggcgag
     2341 ccttctggag aaggtcgccg ggctgaaaga catcatccag gatcgcgagt ccgccaagcg
     2401 tttgctcgat gggagccttc gtgagcttct cgccggcatt cccaatatgc ccgcggacga
     2461 cgtgcccgtg ggcgaagacg agcgggagaa tgtcgagctg cgcaaggtcg gcgagccgcc
     2521 agcgttcgac tttacgccca agcagcattt cgagatcggc gaggaactcg gtctcatgga
     2581 cttcgagacc gcggccaagc tctccggcgc ccgcttcgtc gtgctgaaag gtccgctggc
     2641 gcggctcgaa cgcgcactcg cccagttcat gctcgacctg catacgtctg agcacggcta
     2701 caccgaagtg aacccgccgc ttctcgtgcg cgacgatgcc atgttcggca ccgcgcagct
     2761 gccaaagttt cgtgaggatc agttcgcggc cggcgacgat tactggctca tcccgaccgc
     2821 cgaggtgccg ctgaccaatc tcgcgcgtga cgacattgcc gacgaagcga agctgccgat
     2881 gcgggtcacg gcgggcacgt attgcttccg tgccgaagcc ggtgcggccg gcaaggacac
     2941 gcgcggcatg atccgccagc atcagttcta caaagtggaa atggtctctg tgacgacgcc
     3001 ggaagcgtcc gccgaagaac acgagcgcat gacagcctgc gccgagaccg tgttgaaacg
     3061 actcggactt ccctatcgcg tggtggtgct gtccacgggc gatatgggct tcggcgcgcg
     3121 gaagacttac gacatcgagg tctggctgcc ggggcaggga atgtaccgcg aaatttcgtc
     3181 ctgctcgaac tgcggcgact tccaggcccg gcgcatgaac gcgcgctacc gcccgaagga
     3241 tcgcaaggcg accgagttcg tgcacacgct gaacggctcc ggcgttgccg taggccgtgc
     3301 gctcatcgcc gtgctggaga actaccagga ggaggacggc agcgtgcgcg ttcccgcggc
     3361 gcttcagccc tatatgggtg ggctgacgcg gatcgaagca cccaagtgat cgcgcgccct
     3421 cgtgatcttg cgcctctggc cacacattca tcgtcatgcc cggccgtgtg ccgggcatcc
     3481 acgattggtg tagtgcgaca gggcaagacg tggatggccg ggacaagccc ggccatgacg
     3541 ggtgcttgtt gagatgactc aaaaagagag caaccgaaca aaaccatcgg aaatgactcg
     3601 caaaatgcgc gtcctcatca ccaatgacga cggcatcgaa gcggctggcc tcgacgtcct
     3661 ggaaaagatc gccacggacc tcaccgacga tgtctgggtg gtggcgccgg atacggacaa
     3721 ctcaggcgcc tcgcattctt tgacgttggc tgagccgttg cgtatgcgcg agctcgccaa
     3781 gcgccgcttt gccgtgaagg gcacacccac cgattgcgtc atcatgggcg tgcgctacct
     3841 cctcaaggac aatccgcccg acctcgtgct gtccggtgtc aatcgcggcc agaacctcgc
     3901 cgacgacgtg aactattccg gcaccgtggc cggcgccatc gagggctcgc tgctgggcgt
     3961 gccgtccatc gcgctcagcc tcgccatcgg ctcctatgac acgggcgtgc cggcctggga
     4021 cacgccgatg gttcacggcg cgggcctggt gcgcaagctg ctcgacgcgg gctggcccaa
     4081 gagcaccgtg ctcaacgtca atttcccgga ttgcgagccg aaccaggtca agggcatggc
     4141 ggcgacggtg cagggtttgc gcgacaccgc gctgctcgac atctccgcgc gcatggatgt
     4201 gcgcggcaag gcgtattact ggatcggcat tgccaagcgc ggctccaatc cgccgaagga
     4261 caccgatttg tcggcgatcc gtgccaatct aatttccgtc acgccgctct gtctcgactt
     4321 caccgaccgg cagacgctgg gcgcgttggc cgatgttctc ggcgaaaaag acaaatagtt
     4381 catgggaacg ggccctagaa tcggctgata ctctgccgaa aacccgctcg cgggggaccc
     4441 gcctcgcgga gggggacggg atggcaagcg ctcaggacca aatcggcctc atcatgcagc
     4501 ttcggcgtcg cggtatccgc gaccccaaag tgctgcgcgc catggaggcc gtcccgcgcg
     4561 agacgttcgt cgacgaggcc ttcaccgagc acgcctatca ggacatcgcg ctgcctatcg
     4621 attgtgggca gaccatctcg cagccttttg tcgtcgccta catgaccgag aagcttgggc
     4681 tggagccgac gcacaaggtc ttggaggtcg gcaccggctc aggctatcag gccgccgtgc
     4741 tgtcgcatct gtgccggcgc gtctacacgg tcgagcggtg gcgcgagttg cagaaggtgg
     4801 cggaagcgcg gctggccgat ctcaagatcg ctaacgtcac cacgatcatc ggcgacggct
     4861 ggctgggctg gccgccgcaa gccccgttcg accgcatcat cgtcaccgcc gcggccctcg
     4921 atgcgcccgc cgcgctgctc gatcagttga aagacggcgg ccgcatgatc atcccgctcg
     4981 gcgagaaccg ggactcccag tatctcgtgc agatcgacaa gaccgaggaa ggcatggtgg
     5041 aaacgccgct cctgccggtg cgcttcgtgc ccctggtgca tggccgggtg aagcgcgatt
     5101 gatccgaaat taggcagctg cgctaaggct taatgcgttg tttaccgcca gcgcgcttta
     5161 ctacgcccaa cgggtattgc gtaaagaggg tcaacggcgg atgcgcggag cgttctgttt
     5221 ggagtttggc cgcggcacgg tgcgtgtggt cgcggcagtg ggtcttgcgt ttgcgatggc
     5281 aggctgcagc tccagcgcgc gtttcgatga ttctccctac agtctcagcg acagcgacgc
     5341 tctcgccgat ccgtccacca cggcctcgct gcctgtcccg tctcagtcgg tctacaacgg
     5401 ctccagtggc tcgggccaga tcgcgcgctc gactccgccg cctcaatcca cggccgcgta
     5461 cgacaccagc aagccacgct attacggcac gccgcgttcg gcgaagccgc ctgtgccagc
     5521 gcccatgacg caggcctcgg gcccgcgcat caaggttaaa caaggcgaca cgctttccgg
     5581 tctgtcgcaa cgttatggcg ttcccatcga caccatccag agcgccaaca acttagccga
     5641 tgggcgtttg cgcgaaggcc aggtgttgat catgccggta gcatcgcctc aggctcccgc
     5701 accggcggtt gctcaagccc cagccccagc accggctcct gcgtcaagcg gtgcacgcaa
     5761 ggtcaagacc accaccatcc aggccccggg gacacctgcg ccgactgaaa cgacgcagag
     5821 cgccacaagt gcttcaaagg ttgccagcaa gacgccccaa aacgtcacca gcaccgcaca
     5881 gcttccgaac ccggagccta tgacgggcaa tagcttccgc tggccggtca agggccgtgt
     5941 gatttccggt ttcggcacca agcccgatgg cgggcacaat gacggcatca acatctcggt
     6001 tccgcaagga acgcccgtga aggcggccga gaacggcgtc gtggcctatg ccggcagcga
     6061 gttgaagggc tacggcaatc tggtgctggt ccggcatgcc aacaattggg tgtcggccta
     6121 cgccaacaac gagacgcttc tggtcaagcg cggcgacaag gtgaagcgcg gccaggtgat
     6181 cggcaaggcc ggcaccacgg gctcagtttc aaagccgcaa gtccatttcg aactgcgcaa
     6241 aggctcacgc cccgtcgatc cgacgaagta catgagcagc atggccgctc aagccaacta
     6301 gccgtcaagc tgcttgccga ggcgtcccgc caagtcctga atgaactgcc aagcgacccg
     6361 gccagagcgg ctgccgcgtc cgcgcgccca ctcaagcgct tcctgggtca agtgcgcgtc
     6421 ggggacgtcg agcccgtagt gatcggcata gctccgcacc atggcgagat attcatcctg
     6481 gctgcaattg tggaagccga tccacagacc aaaccggtcg gacagggaca gcttctcctc
     6541 gacggcctcc gatgggttga tggccgtgga gcgctcgttc tccaccatct cgcggggcat
     6601 caggtggcgc ctatttgacg tggcataaag cagcacgttg tcgggacggc cttcaatgcc
     6661 gccttccagc accgccttga gcgatttgta cgaggtatcg acggcctcga aggagaggtc
     6721 gtcgcagaag agaatgaagc ggtactgggg cgcggcgcgc aaataagtca aaagccgggg
     6781 cagggcggcg atgtcctcgc ggtggatttc cacgagcttc aagccgggaa aggccccatg
     6841 cgccgccttg ataagggagc tcttgcccat gccccgggag ccccacagga gagcgttgtt
     6901 ggccggaagg ccacgcgcaa aacggtcggt attggctaga agcgcgttgc ggttgcggtc
     6961 gatgcccttg agcagctcca ggggcacccg gttgaccgaa gagaccggcg cgaagcgctc
     7021 cgattcggcc tgaaagacga aggcgccggc gtgggcgaaa tctggcgttt gcggccgggc
     7081 ggggccaagt cgctccagcg catgggcaat tcgctccata aatcccgaaa ttggctcctg
     7141 aaagttcatg cgggccccgt tgcaaaggcg ttcaggctca gtatagtccg gcccaatcgc
     7201 gcgataagag cctgctcgcc acgccgcgag acacgccaac ccggccgaaa gcggccttac
     7261 cttagccaag caggggacac tcgcaatgga cggtttcgca tacgcgcaag caagcggtgg
     7321 agcattcgag atgctgaaca gcttgctcgt cccaaccatc ctcatcatcg gcatcatgta
     7381 tttcctgatg atccggccgc agcagaagcg gatgaaggag catcgcgaga tgatcgcctc
     7441 cattcgccgc ggcgacaccg tggtgacctc ggggggcatc ctcggcaagg tgacgaaggt
     7501 ggaggataac gagcttcagc tcgagatctc cgacggcgtg cgcatcaagg ttctgcgtcc
     7561 gaccgtgtca gaggtgcgcg ggaaggatga ggcgggcggc gcttcatcgt cgaagtccga
     7621 agccaaatag tcccgcaaaa cgtcgtctga tctggagagt taaacccggc aatgctgcat
     7681 ttccaaaaat ggaagctgat cttggtgttc ggtattgtgc ttgccggctt cattttcgca
     7741 attcccaatc tgtttccgtc ttcgagcctt caagggcttc cgagttggtt tcctcacaag
     7801 caagtcaatc tcggcctcga ccttcagggc ggtgcgcatc tgctctaccg gatcgacgat
     7861 aaggaactgg tcgacgattg gctcggcaca atcagaggtg atgtgcgcga gggcttgcgc
     7921 agtaatcgta tcggctacac cgacctctcc cagaacgtag cgggccgcag cgtgtcggta
     7981 cgcatccgca agcccgacga catggacaag gcctacacgg agctgaagaa acaatccgtg
     8041 ccggtcggtg gcgatgtgtt cggcgggttc gccggcaaca atctcgaagt gacgcgcgac
     8101 gccgacaaga tcacgttgac cgtgactgag gttggcctga cggacaggat caactcggcc
     8161 atccaagcct cgatcgaaac cattcggcgc cgcattgacg ccttcggcac caccgagccc
     8221 agcatccagc gcgaaggacg caaccgcatt ctcgtccaag ttcccggcgt gacggacgtg
     8281 cagcggctga agtccctgat cggtgagacc ggaaagctcg aattcaagat ggtcgattcc
     8341 acggtgaatg ccgccgaggt cgaggtcagc aagaaggtac cgccaggaga cgagctcctc
     8401 tattcgtccg acgacccgcc cgttccttac gtgctcaaag accgcgtctt ggtcagcggc
     8461 gaaaacctgg tcgacgcgca gccgggattc gattcacgga ccggcgagcc cgtggtgacg
     8521 ttccgcttcg atgcggcggg cgccaagcgt tttggccgcg ccacccagga gaatgtcgga
     8581 ctgccgtttg ccatccttct cgacaacaag gtgatctcgg cccccgtcat ccgcgagccg
     8641 atcctcggcg gtactggaca gatcagcgga aacttcacgg tgcaggaggc gaacgacctc
     8701 tcggtgcttc tgcgctccgg cgcgctaccc gccaagctca ccgtcattga agagcggacg
     8761 gtcggtgcga gcctcggtgc ggactcaatt gaaagcggca agaaggccgc catcgcgggc
     8821 ttgctgctcg tcgtgctgtt catgttcgcc gcttacggcc tgttcggctt ctttgcgaat
     8881 ctggcgctcc tggtgaatat cgcgctgatc ttcgcggtgc tatcgctcat cggcgccacg
     8941 ctgacgctgc ccggcatcgc gggcatcgtg ctcgtcatcg gtatcgccgt ggatgcgaac
     9001 gtgctcatca acgagcgtat ccgagaggag aagagggcgg gcaagtcgcc tattgccgcc
     9061 atcgactccg gttattcgcg ggcgctgatc accattatcg actccaacgt caccacgctg
     9121 atcgccgtgc ttgtgctgtt ctggctcggg tcgggcccga tccgtggttt tgccgtcacg
     9181 ctgacgatcg gtgtgttggc gtccatgttc accgccgtca ctgtgacgcg catgatgatt
     9241 gcttactggt tgcggtggac gcggccaaaa gagctcccaa tctagggtta tccaagaatg
     9301 ttccgcggca tcaccttcat tcccgccgac accaagatcg atttcgtcgg tcaacgtgcc
     9361 gttacgtggt tcgtctccgc ctttctgacg gtcgtaccgc tgatcctcgt ggctacgatc
     9421 gggctcaata tgggcatcga cttccaaggg ggcaccctga tcgaggtgaa gaccaaggag
     9481 agtccggcaa acctcgccga cattcgcggc aaggtcagtg gtctcggttt gggtgaggtg
     9541 cagatccagg aattcggcga gcccgacgag gtgctgatcc gcattgcctc gcagcccact
     9601 gaagaagagc agcaagagtc catcgccagg gtgaagaacg cgctggggga cagtgtcgag
     9661 tatcgccgta cggaaattgt cggccccacc atttcgggcg agctcatcgc cggcggcacg
     9721 ctcgccgtgg tggtcgcgat gatcggcatc atgatctatg tgtggttccg tttcgaatgg
     9781 cagttctccg tggctgccgt cgcatcgctc gttcacgatg tcaccgcgac tatcgggctt
     9841 tatgcgctgc tccagttgga gttcaatgta tcgagcatcg cggcaatcct gaccatcatc
     9901 ggttactccc tgaacgacaa ggtcgtgatc ttcgaccgca tcagagagaa tttacgcaag
     9961 tacaagcgta tgccgctcaa gaatctgctc gacctgtcca tcaacgagat gctgtcgcgg
    10021 gcgatcctca cccatgtgac gaccctgctg gccatgatgc cgttcctgtt cttcggcggg
    10081 gaggctatct acggctttgc cttggccatg tgtttcggca tcattgtcgg cgcttactcg
    10141 tcgatcttcg tggcgtctcc gatgcagctc atcctcggcg tgcagcgcga ggccttttct
    10201 ccaagggcgg cggcacgcgc caatccgtga ggtagccctg tgcgtgacga cgagcggcgc
    10261 gacgtggtca gacgcatcgc ccgcgacgcc gatcccgatc gcgccatcgc gaccctgttt
    10321 gcaccggccg aaacgcgcgc cgacctcttt gcactctttg ccttcaatgc cgaactggcc
    10381 cgcatcgccg atcaggtgag tgagccgggg cttggcgcca tccgcttgca atggtggcgc
    10441 gaggccatag aacgggcggc aagcggcgac gtcacgggta atccggtcgc cgacgcgttc
    10501 ggcgaagttc tggcgcggcg gacattatct cgtgagcgca ttgcgggcct gatcgatgcg
    10561 cgcagtttcg atgtcggcga gacggtgatg ccggatacgc ggacgctcga cgcctatctg
    10621 ttcgacacca cgggcgggct gttcgcgctt gccgcggaca ttgtcggcgc cggcggcgaa
    10681 aaacgcgatc tggtggtgga ggctggcgcg cgcgcttatg ggctggtccg cgtgatgcgc
    10741 agcctgccgg tgttggccgc caaagggcgc acgtatttgg cgtctgatgc actggcccat
    10801 catgggacct cgccccaggc gattttcgcc ggggagacga acgacggcct tgaggtgctg
    10861 ctcaccgaga tgcgggagga gtcgcgtgcg gctctaaagg aggcgcagtt ccacttgttc
    10921 ggcgaaggca tgaagggggc cggtctcgat gaggcgggcc gcgccacgtt tctgccgctc
    10981 tgccttgtcg agccctatct cgcggcgctg gccaaagtcg gcgatccatt gcgccagacc
    11041 gcgcgcatca acccgctcgc ccggctgtgg cacctgatgc ggtcgcgttg agccgactat
    11101 tcggcggcga tcgtcgcggt gccttccagc caagcggcga gatcggccag tgcgcgggcg
    11161 gacatcgcct gcttgcgggc gagggacttc gcgccacgct tcgccttgcc cggtgggcgc
    11221 ggcggcgcgg gcaccggcgg gaacagaccg aaattcacat tcatcggttg gaagctgcgc
    11281 gcggcgcctt cgccgtcggc gctcaaatga ccgccagtga tatgggtgag gagggcgccg
    11341 agcgctgtcg ttggcggcgg cgggagaagc ggcttggccg ttcgctccgc tgctgcgaag
    11401 cgtcccgcga tcaagccaat tgcggcggac tccacatagc ctt
//
LOCUS       DBNHVU010000127         6126 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_791574, whole genome shotgun sequence.
ACCESSION   DBNHVU010000127 DBNHVU010000000
VERSION     DBNHVU010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_791574"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..338
                     /locus_tag="AC16Y7_05040"
     CDS             63..338
                     /locus_tag="AC16Y7_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR5708778.1"
                     /translation="MNKAELVAHVAKESELSKDAAEKAVDATFKNIEKALSGGDTVRI
                     VGFGNFQVADRKASIGRNPRTGEAVQIPASRVPKFRAGKALKEAVNA"
     gene            421..496
                     /locus_tag="AC16Y7_05045"
     tRNA            421..496
                     /locus_tag="AC16Y7_05045"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:454..456,aa:Val,seq:tac)
     gene            651..727
                     /locus_tag="AC16Y7_05050"
     tRNA            651..727
                     /locus_tag="AC16Y7_05050"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:685..687,aa:Asp,seq:gtc)
     gene            768..1133
                     /locus_tag="AC16Y7_05055"
     CDS             768..1133
                     /locus_tag="AC16Y7_05055"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR5708779.1"
                     /translation="MGQLLQDYLPLVIFIGIAFVISTILLVVPFVIAVRNPDPEKVSA
                     YECGFDAFDDARMKFDVRFYLVSLLFIIFDLEVAFLFPWAVAFGDIGALGFWSMIVFL
                     GVLTIGFIYEWRKGALEWD"
     gene            1124..1681
                     /locus_tag="AC16Y7_05060"
     CDS             1124..1681
                     /locus_tag="AC16Y7_05060"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NuoB/complex I 20 kDa subunit family protein"
                     /protein_id="HIR5708780.1"
                     /translation="MGLMPEVRFETEGKGGGQITAGATTFGTFSDELADKGFLLTTAD
                     DLINWSRTGSLMWMTFGLACCAVEMMQASMPRYDLERFGFAPRASPRQSDVMIVAGTL
                     CNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCP
                     PTAEALVYGVLLLQKKIRRTGTIER"
     gene            1712..2338
                     /locus_tag="AC16Y7_05065"
     CDS             1712..2338
                     /locus_tag="AC16Y7_05065"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR5708781.1"
                     /translation="MDDALNKLGAYLSGKLGPKLVRFDVALGELTIEVKRDSVRDVIT
                     FLRDDERCRFASIIDICGVDYPDRDERFDVVYHFLSPWLNHRVRVRVPASADTPVPSI
                     VDLFPGANWFEREAYDLYGIEFEGHPDLRRLLTDYGFQGHPLRKDFPLTGFVELRYDD
                     ELKRVVYEPVKLMQEFRNFDYLSPWEAMDTAIPGDEKAGPAEEPKGDS"
     gene            2344..3567
                     /locus_tag="AC16Y7_05070"
     CDS             2344..3567
                     /locus_tag="AC16Y7_05070"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018857056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HIR5708782.1"
                     /translation="MAETDTKDVVDSGKREGDDRTFTINFGPQHPAAHGVLRLVLELD
                     GEVVERVDPHIGLLHRGTEKLIEHKTYLQAVPYFDRLDYVAPMNQEHAFALTVEKLLG
                     LSVPKRAQLIRVLYSEIGRLLSHLLNITTQALDVGALTPPLWGFEEREKLMVFYERAS
                     GSRMHAAYVRPGGVHQDLPPALVDDIYAFCDPFLKVCDDIEGLLTHNRIFKQRNVDIG
                     VFSEKDAWAYGFSGVMVRSTGAAWDLRKSQPYECYEELEFDSPIGKNGDCYDRYLLRM
                     EEMRQAIRIMKQCCEMLTTTPGPVTVDNHKMVPPHRAKMKGSMESLIHHFKLYTEGYH
                     VPKGEAYAAVEAPKGEFGVYLVADGSNRPYRCKIRPPSYPHLAALDFLCRGHMLADVS
                     AVLGSIDVVFGEIDR"
     gene            3691..4368
                     /gene="nuoE"
                     /locus_tag="AC16Y7_05075"
     CDS             3691..4368
                     /gene="nuoE"
                     /locus_tag="AC16Y7_05075"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HIR5708783.1"
                     /translation="MSVRRLAADQPEGFAFTPENLKWAKAEIAKYPEGRQASAVLSLL
                     WRAQEQNDGWISEPALRYVADMLGMPYIRVYEIVTFYTMYNLAPVGRYYVQVCGTTPC
                     MLSGAEDIKDVCRKHIGPKDTVSDDGMFSWTEVECLGACVNAPMAQINKYYYEDLTPE
                     SFEALLGKLRKGENVEPGPQDGRQAAAPEGGPNTLTDPALYAKDKSGPEPGYAGTSDE
                     TAKARSR"
     gene            4368..5657
                     /gene="nuoF"
                     /locus_tag="AC16Y7_05080"
     CDS             4368..5657
                     /gene="nuoF"
                     /locus_tag="AC16Y7_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR5708784.1"
                     /translation="MLPDKDRIFTNLYGLHSWRLPAARARGAWDGTKSFIARGHDGVI
                     DEVKASGLRGRGGAGFPTGLKWSFMPKNDSRPSYLAVNADESEPGTCKDREIIRHDPH
                     LLIEGALLAAFAMRAHTAYVYIRGEFIAEREIMQAAIDEAYAAKLVGKNNVNGWDFEI
                     FVHHGAGAYICGEETALLESLEGKKGQPRLKPPFPANCGVWGAPTTVNNVETLASVPD
                     IMRRGATWYAALGRPGNTGTKLYCISGHVNKPCTVEEEMGIPLRELIDTYAGGVRGGW
                     DNLLAVIPGGSSVPCLPASLCDTVLMDYDSLKAVDTALGTAGVIVMDKSTDIIRAIAR
                     IAYFYKHESCGQCTPCREGTGWMWRVLERMADGNARKGEIEMLLDVSHQVEGHTICAL
                     GDAAAWPVQGLIRHFRHVIEERIDAAATKPSVPVAAE"
     gene            5666..>6126
                     /locus_tag="AC16Y7_05085"
     CDS             5666..>6126
                     /locus_tag="AC16Y7_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR5708785.1"
                     /translation="MPKLIINDKEHDVPDGITLIQACELAGVEIPRFCYHERLSIAGN
                     CRMCLVEVIGMPKPVASCAMSVNDLRPGRDGTPPTILTHSETTKKAQEGTLEFLLINH
                     PLDCPICDQGGECDLQDQTRAFGFDAGRFHDNKRAVEDKYLGPLIDTCMTR"
BASE COUNT         1191 a         1937 c         1843 g         1155 t
ORIGIN      
        1 aaagcggcct tgagggttaa gtgacgccct gcgatcgggg ccaagcgaaa ggatggagac
       61 ccgtgaacaa agctgagctg gtcgcccatg tggcgaaaga atccgaattg tcgaaggacg
      121 ccgccgagaa agctgtcgac gcgacgttca agaacatcga gaaagccttg agcggcggtg
      181 acaccgttcg cattgtcggc ttcggaaatt tccaggtcgc ggatcgtaag gcgtccattg
      241 gtcgcaatcc gcgcaccggc gaagcggtcc aaattccggc ctcccgcgtg ccgaagttcc
      301 gcgctggcaa ggcgctcaaa gaggctgtga acgcgtaaac acggattacg cgatttctga
      361 ggatttcctg gcgaggcctc gccctttgcg tgaggcttcg cctttcttat ttgaaggtga
      421 gggcggttag ctcagttggt agagcgccac gtttacaccg tggatgtcgg cagttcgagc
      481 ctgtcaccgc ccaccattct cgccatgcat gacgctaacg gttggtccca ggtcaacctg
      541 cacgcgccgc aacgtcccat cctcgtgtct tcgctaggct cgccttgact tggggaatcc
      601 caggccgtac atgctgaaga tcgcctctgg ccgtggttga cggcccggta gggggtgtag
      661 ctcagttggt tagagcgccg gcctgtcacg ccggaggccg cgggttcgag tcccgtcact
      721 cccgccattt tggccccgtg aatgcaaaag aaggcttcgg aagagccatg ggccagctgc
      781 ttcaagatta ccttcccctc gtcatcttca tcggcatcgc attcgtcatc agcaccatcc
      841 tgctggtcgt gcccttcgtc atcgccgtac gcaatccgga tcccgagaaa gtctcggcct
      901 atgaatgcgg cttcgacgcg tttgatgacg cgcggatgaa attcgacgtg cgcttctatc
      961 tcgtgtcgct gcttttcatt atcttcgact tggaagtcgc atttctgttc ccctgggccg
     1021 tcgccttcgg agacattggc gcgctcgggt tctggtcgat gatcgtgttc ctcggcgtgc
     1081 tcacgatcgg cttcatttat gagtggcgga aaggcgcgct cgaatgggat tgatgcctga
     1141 agtcaggttc gagacggaag ggaagggcgg cggccagatc acagccggcg ccactacatt
     1201 cggcaccttc tccgacgaac tcgccgacaa gggctttcta ctcaccaccg ccgacgacct
     1261 cataaactgg tcgcgcaccg ggtccctcat gtggatgacg ttcggcctcg cgtgctgcgc
     1321 cgttgaaatg atgcaggcgt cgatgccccg ctacgatctg gagaggttcg gcttcgcacc
     1381 gcgcgccagt ccgcgtcagt ccgacgtgat gatcgtcgcc ggcacgctct gcaacaaaat
     1441 ggcgcccgct ctgcgcaagg tctacgacca gatgcccgag ccccgctacg ttatctctat
     1501 gggctcctgc gccaatggcg ggggctacta tcactactcc tactctgtgg tgcgcggctg
     1561 cgatcggatc gtacctgtgg acatctacgt tcctgggtgt cctccaacgg ccgaagcgct
     1621 cgtctatggc gtactactcc tacagaagaa gatcaggcgg acaggtacga tcgaacgctg
     1681 acctccagtc cactctgatc ctgggtattt gatggacgac gcactcaata agctgggcgc
     1741 ttacctctcc ggaaagctcg ggccgaaact cgtgcggttc gacgtggcac tcggcgaact
     1801 caccattgag gtgaagcgcg acagcgtgcg tgacgtcatc acgttcttgc gcgacgacga
     1861 gcgctgccgc ttcgcatcga tcatcgatat ttgcggcgtg gactatcccg accgcgatga
     1921 gcgtttcgat gtcgtctatc acttcttgag cccctggctt aatcaccgcg tccgtgtccg
     1981 tgtgccggca agcgccgaca cgccagtccc gagcattgtc gatctgttcc ccggggccaa
     2041 ctggttcgag cgcgaggcgt acgaccttta cggcattgag ttcgaaggcc acccggacct
     2101 gcgccggctg ctcaccgatt acggttttca aggtcatccc ttgcgtaaag acttcccgct
     2161 caccggcttc gtcgagttgc gctacgacga cgaactcaag cgcgttgtct atgagcccgt
     2221 gaagctcatg caagagttcc gcaatttcga ctatctgagc ccttgggaag ccatggacac
     2281 ggcgatccct ggtgacgaga aggcgggccc tgccgaagag ccgaaggggg acagctaaag
     2341 ccgatggccg agaccgacac caaagacgtc gtggacagcg gcaagcggga aggggacgac
     2401 cgtaccttca ccatcaattt cgggccgcag catcctgcgg cacatggcgt gctgcgcctc
     2461 gtgctcgaac tggacggcga ggttgtcgag cgcgtcgatc cgcatatcgg gctgctgcac
     2521 cgcggcaccg agaagctgat cgagcacaag acctatctcc aagccgttcc gtatttcgac
     2581 cggctcgatt acgtcgcgcc gatgaaccag gagcatgcct tcgcgctcac cgtggagaag
     2641 ctgttgggac tgagtgtccc caagcgtgcc cagctgatcc gcgtgctcta ctccgaaatc
     2701 ggtcgcttgt tgtcgcatct tctgaacatc accacgcaag ccttggatgt cggcgcgctg
     2761 acgccgccgc tctggggctt cgaggagcgc gagaagctaa tggtgttcta cgagcgggcc
     2821 agtggcagcc gcatgcatgc ggcctatgtc cgccccggcg gcgtgcacca ggatctgccg
     2881 ccggccttgg tcgacgacat ctacgccttc tgcgatccct ttcttaaagt ctgcgacgac
     2941 atcgaaggac tgctgaccca taaccgcatc ttcaagcagc gcaatgtgga tatcggcgtg
     3001 ttcagcgaga aggacgcttg ggcctatggc ttttcgggcg tgatggtccg ctccacgggc
     3061 gcggcgtggg acttgcgcaa gagccagcct tacgagtgct acgaagaact cgagttcgac
     3121 agtcccatcg gcaagaacgg cgactgctac gaccgttatc tgctccgcat ggaagagatg
     3181 cgccaggcca tccgcatcat gaagcaatgt tgcgagatgc tgacgacgac gccgggcccc
     3241 gtcaccgtcg acaaccacaa gatggtgccg ccgcaccgcg ccaagatgaa gggctccatg
     3301 gaatccctca tccaccactt caagctgtac accgagggct accacgtccc gaaaggcgag
     3361 gcctacgccg ccgtcgaagc gcccaagggc gaattcggcg tctatctagt cgccgacggg
     3421 tcgaaccggc cttaccgctg caagatcagg ccgccgagct atccccatct cgcggcgctc
     3481 gatttcctct gccgcgggca catgctggcg gatgtgtcgg cagtgctggg gtcgatcgat
     3541 gttgtctttg gggaaatcga ccgatgacga tttgtgcatt gcgaaatcgt tcccggtcgg
     3601 caagactctt gaaggcctta cggaagtcag ttcacagcga tttgcgcttg tctgggcacg
     3661 acccggcgcc gatcgcacaa gaagattgag atgagcgtcc gacgcctcgc cgccgatcag
     3721 cctgagggct tcgccttcac gcccgagaac ctgaaatggg ccaaggctga gatcgccaaa
     3781 tacccggaag gccgccaggc ttcggccgtg ctgtcgctgc tatggcgcgc ccaggagcag
     3841 aacgacggct ggatctccga gcctgcactg cgctacgtcg ccgacatgct cggcatgccc
     3901 tatatccggg tctacgagat cgtcaccttc tacacgatgt acaatctggc gccggtcggc
     3961 cgctactacg tgcaagtgtg cggcacgacg ccttgcatgc tcagcggcgc ggaggacatc
     4021 aaggacgtct gccgcaaaca catcggtccg aaggacactg tcagcgacga cggcatgttc
     4081 tcctggaccg aagtcgaatg tcttggcgcc tgcgtgaacg cgcccatggc gcagatcaac
     4141 aagtactatt acgaggacct gacgccggag agtttcgagg cgctgctcgg caaactccgc
     4201 aaaggggaaa acgttgagcc cggcccacaa gacggccgcc aggccgcagc ccctgaaggc
     4261 ggccccaaca cgctcaccga tcctgcgctc tacgctaagg acaagtctgg ccccgagccg
     4321 ggttatgcgg gtacgtccga cgagacagcc aaggcgaggt cgcgctgatg ctcccggaca
     4381 aggatcgcat cttcaccaac ctctatggcc tgcacagttg gcggctgccg gcggcgcggg
     4441 cgcgtggcgc ctgggacggc accaagagct tcatcgccag gggccatgac ggcgtgatcg
     4501 atgaggtgaa ggcatccggc ttgcgcggcc ggggcggtgc cggattcccg acaggtctca
     4561 aatggtcgtt catgcccaag aacgacagcc gcccgagcta cctcgccgtc aacgccgatg
     4621 aatcggagcc cggcacctgc aaggaccggg agatcattcg ccacgacccg catcttctca
     4681 tcgagggcgc gctgctcgcc gccttcgcca tgcgcgcgca taccgcttac gtctatattc
     4741 gcggcgagtt catcgccgaa cgcgagatta tgcaagcggc catcgacgaa gcctacgccg
     4801 ccaagctcgt cggcaagaac aacgtcaatg gctgggactt cgaaattttc gtccatcacg
     4861 gggccggggc ctatatctgc ggcgaggaaa cagcgctcct cgaaagcctc gaaggcaaga
     4921 agggtcagcc gcgcctaaag ccgccatttc ctgccaattg cggcgtgtgg ggcgcaccga
     4981 ccacggtcaa caacgtggag accctcgcct cggtgccgga catcatgcgc cgtggtgcca
     5041 cctggtacgc cgccctcggc cgtcccggta acaccggcac caagctctat tgcatctccg
     5101 gccacgtgaa taagccctgc accgtcgaag aagagatggg cattcccttg cgcgagctga
     5161 tcgacactta cgcgggcggg gtacgcggcg gctgggacaa tctcctggcg gtcatcccgg
     5221 gcggctcgtc ggtgccgtgc cttcccgcca gcctctgcga caccgtgctg atggactacg
     5281 acagtctcaa agccgtcgat accgcacttg gcaccgccgg tgtcatcgtc atggacaagt
     5341 cgaccgacat catccgggca atcgcgcgca tcgcctattt ctacaagcac gagtcttgcg
     5401 gccagtgcac gccctgccgc gaaggcacgg gctggatgtg gcgcgtgctg gagcgcatgg
     5461 ccgacggcaa tgcacggaag ggtgagatcg agatgctgct cgacgtgagc catcaagtcg
     5521 aaggccacac gatctgcgcg ctgggtgacg ccgccgcctg gccggtgcaa ggcctgatcc
     5581 ggcatttccg tcacgtcatc gaagaacgca tcgacgcggc agcgacaaaa ccgtccgttc
     5641 cagtggccgc ggagtagggg caagcatgcc gaagctgatc atcaacgaca aagagcacga
     5701 cgtcccggac ggcatcacgc tgatccaggc gtgtgagctc gccggcgttg agatcccgcg
     5761 cttctgttac cacgagcgcc tgtccatcgc cggcaattgc cgcatgtgcc tcgtcgaagt
     5821 gatcggcatg ccaaagcccg tggcctcctg cgccatgtcg gtgaacgatc tgcgccccgg
     5881 acgcgacggc acgccgccaa ccatcctgac gcactcggag acgaccaaga aggcgcagga
     5941 aggcacgctc gaattcctgc tgatcaatca cccgcttgat tgtccgatct gcgatcaggg
     6001 cggcgaatgc gacctgcaag atcaaaccag ggcgttcggc ttcgacgccg gccgctttca
     6061 cgacaacaag cgcgccgtcg aagacaaata tctcggcccg ctcatcgaca cctgcatgac
     6121 gcgttg
//
LOCUS       DBNHVU010000128         9737 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1608508, whole genome shotgun sequence.
ACCESSION   DBNHVU010000128 DBNHVU010000000
VERSION     DBNHVU010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 9737)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9737)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9737
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1608508"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="AC16Y7_05090"
     CDS             complement(<1..294)
                     /locus_tag="AC16Y7_05090"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR5708786.1"
                     /translation="MTRKGPSSVLIIEARFYEDIADELARGATEELRGNQVEYERISV
                     PGALEIPLALSLALANGFVGRAGRHRACIALGCVIRGKTSHYEIVARESAAGLN"
     gene            complement(291..1430)
                     /gene="ribB"
                     /locus_tag="AC16Y7_05095"
     CDS             complement(291..1430)
                     /gene="ribB"
                     /locus_tag="AC16Y7_05095"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HIR5708787.1"
                     /translation="MTAVTKASDRASYHQYLSPIEEVVEDARNGRMFILVDAEERENE
                     GDLVIPAQMATPDAINFMAKHGRGLICLALTSERTNQLRLELMSRSNRSRHSTAFTVS
                     IEAREGISTGISAHDRSHTVSVAIDPTKDYRDVMTPGHVFPLLAKDGGVLVRAGHTEA
                     AVDIARLAGLNPAGVICEVMNDDGTMARLPDLVSFAQDHDLKIATIADLIAYRLRYDH
                     LVKQVAKTTLDSAIGGPFDAKVYATTVDPAEHIALVKGDVSSGEPVLVRVHAMNTLDD
                     LLASGGKRETPLHKAMDIIGREGRGVIVLIRESRPNAISARLNQSGNGQSKPRLLEYG
                     IGAQILLDLGVREMVLLSNSPARKIVGLEGYGLKVAGQRGLEPGA"
     gene            complement(1427..2053)
                     /locus_tag="AC16Y7_05100"
     CDS             complement(1427..2053)
                     /locus_tag="AC16Y7_05100"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HIR5708788.1"
                     /translation="MFTGIVSGVGTIFSRKGATYTIACPFKRKSLEEGASIAFDGCCL
                     TLVDIDKGKDKGAVVTVDVSNETMAHTTLGAWDVGQAINLERALALGEELGGHIVTGH
                     VDGRAKVVSRYPDGDCVRFLLQAPSDIGRFIAPKGSVALNGVSFTVNEVDGQRFGINV
                     IPYTLTHTTWGDRQPGDLVNIEADLLARYAARLAQAEDSSTQPKDKKS"
     gene            complement(2026..3177)
                     /gene="ribD"
                     /locus_tag="AC16Y7_05105"
     CDS             complement(2026..3177)
                     /gene="ribD"
                     /locus_tag="AC16Y7_05105"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR5708789.1"
                     /translation="MSPQDHHQPQDEYRMAQALRLASRGLGLAWPNPAVGAVVVTPSG
                     EIAGRGWTAPGGRPHAEAIALNHAGAKAEGSTVYVTLEPCAHESVRGMACTDALLEAK
                     PKHIVIGLRDPDPRTAGAGIERLRAAGIEVTEGVLPEEVARVTLGHLMRVAEGRPAVT
                     LKLAVGADGLVPEGDGKPVWVTGPEARAHAHLLRARHDVILVGRGTVVTDNPALTCRL
                     PGMAWRSPIRAVLDGRLRTPPDATLFDDIMVPVWLLCAAGVDQPNAELLHDRGAEIVP
                     VPVDNHGRIDVQDALETLSHRGITSILVEGGPSVARAFLDADMVDEVVVYQGRGQAGS
                     DGLTPFAGDGLDRLAASGHFTLSATRTFGPDRMTSWRRVRSCSQALSAA"
     gene            complement(3193..3696)
                     /gene="nrdR"
                     /locus_tag="AC16Y7_05110"
     CDS             complement(3193..3696)
                     /gene="nrdR"
                     /locus_tag="AC16Y7_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR5708790.1"
                     /translation="MRCPYCGSTNTQVKDSRPSEDHTTIRRRRICSDCGGRFTTFERV
                     QLRELTVIKRSGRRIPFDRDKLMRSVQIALRKRPVEPERTEHMVSGIVRRLENMGESD
                     IRSQTVGRLVMEALKGLDDVAYVRFASVYKNFREAKDFENLLGELAGTEGEDADVDID
                     EPTAEDD"
     gene            complement(3737..5044)
                     /gene="glyA"
                     /locus_tag="AC16Y7_05115"
     CDS             complement(3737..5044)
                     /gene="glyA"
                     /locus_tag="AC16Y7_05115"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0006545 - glycine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR5708791.1"
                     /translation="MTASDIQRAAARESAFFTKHLEEADPEVWDAIELERGRQQNEIE
                     LIASENIVSRAVIDAVGTVLTNKYAEGYPGRRYYGGCQYVDVVEQLAIDRAKRLFDCN
                     YANVQTHSGSQANQAVMLALIKPGDTVLGMSLAAGGHLTHGAPPNLSGKWFNAVQYGV
                     TQNTNLIDYDEVERLANEHKPALIIAGGSAYPRQIDFKRFREIADAVGAYLLVDMAHF
                     AGLVAAGLHPSPLPYADVCTTTTHKTLRGPRSGMVVSNNEDIGKKINSAVFPGLQGGP
                     LMHVIAGKAVAFGEALQPSFKLYAQSVIDNAKALGETLVNGGLALVSGGTDTHLILVD
                     LRPKKLTGKAAEIALGRAHITCNKNGVPFDPEKPMVTSGIRLGSPAGTTRGFGVPEFI
                     SVGGMILEVLDGLAANGDEGNSAVEESVKQRAIELCDRHPIYE"
     gene            complement(5293..5811)
                     /locus_tag="AC16Y7_05120"
     CDS             complement(5293..5811)
                     /locus_tag="AC16Y7_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese family protein"
                     /protein_id="HIR5708792.1"
                     /translation="MSLPSISPQDAKKLIDQGATLVDIRGADERAREHIPGSQHGPLG
                     PATNFAGVKGPIVFHCRSGMRTANNAGRLKDAAQSEAYSLTGGIEAWKKAGLPVVADK
                     SPPIEINRQVMIGAGTLVLLFVLLGAFAAPGFYVLAGFVGAGLVFGGATGWRGMAKLL
                     QVMPWNRTPAGA"
     gene            complement(5857..6174)
                     /locus_tag="AC16Y7_05125"
     CDS             complement(5857..6174)
                     /locus_tag="AC16Y7_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5708793.1"
                     /translation="MPRAPDDLATFEANATMVATMLRALANERRLMVLCKLVEWGEGS
                     VGALAEAIGLSQSATSQHLAKMRDEGLVAFRREGQTLWYRIAEPRIELLLAYLHKLYC
                     SDD"
     gene            complement(6348..7724)
                     /locus_tag="AC16Y7_05130"
     CDS             complement(6348..7724)
                     /locus_tag="AC16Y7_05130"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR5708794.1"
                     /translation="MPKSTDIIRYGSAIAAAGLAVAVLVATVQPSGAGPFNIFKNKNS
                     NSQQAPQANSGGGGGGGLFGGLFGGGRSRSPGHESHKSELPRNDPEVAMIVNPGLGSP
                     TAARQNIAATKAAIKKYKAIVAQGGWPTVPAKAMKPGSRGQEIVALHRRLEIGGDLVG
                     MSTPDRYDAAVVAAVKRFQVRHGLPPTGVIDSKATIRALNVPASVRLAQLQGSLKRLQ
                     KLAPRTGSRYVLVNIPAAQVEAVEGGRVAQRHAAVVGKIERPTPELSSKINQVKFNPY
                     WYVPRSIIHKDLVPKGRQFAQRGQDMLAAYHMEAFDRQGNQLDPRQINWFGEEVYSYN
                     FRQRPWEENSLGFVKIDFPNKDAVYLHDTPLKGLFGKAVRFESSGCVRVHNVDKLVAW
                     TLRGNPQWNLSQVQAMKHNRQQVNVPTKRIPVHLAYISAWATPDGRVHFRPDIYSHDT
                     SGAYASAY"
     gene            complement(8085..8597)
                     /gene="ldtR"
                     /locus_tag="AC16Y7_05135"
     CDS             complement(8085..8597)
                     /gene="ldtR"
                     /locus_tag="AC16Y7_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LdtR"
                     /protein_id="HIR5708795.1"
                     /translation="MNNVALNIGTHVSTDGAEDLKGHYLVSLKLVERLHRLLLDVIKD
                     EFERVGRGDVNSVQALLLYNIGDAELTAGELTSRGYYLGSNVSYNLKKLVDAGYIHHQ
                     RSTTDRRSVRVSLTEKGKEVSKVIDGLYQRQLRSLEQVGGVVADDLETLNKALIRLER
                     FWTDQIRYQL"
     gene            complement(8744..>9737)
                     /locus_tag="AC16Y7_05140"
     CDS             complement(8744..>9737)
                     /locus_tag="AC16Y7_05140"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF027439.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR5708796.1"
                     /translation="NALTHEMFAALSENYQRWAPAPNIYGVVMQSTHPTVFCSGGDLK
                     VLSALNEKDAIGTIREFYRAAYSHVWVLEKFIRPNVPLINGLVYGGGVGISLLGTHCV
                     AAEGYRFAMPQVGIGFLPDIGGTYFLPRLMGWMGSYLALTGATIGPADAHRLRLVSHY
                     IPSAQFDVIKTALADNHPIDRLLDGLHRDPGEGELVRQTPAINRIFSADSVEEIMRRL
                     DAEDGEDADWARQTAAELRKKSPTSLKIAFKQMQVGKSLSLNEALKLEFRLASHLAGT
                     HDFREGIDARINGKGREPQWQPARLEDVSDAEIDRLFSTPIDGELKLIEPNCIS"
BASE COUNT         1757 a         3040 c         3021 g         1919 t
ORIGIN      
        1 attgagccct gcggcggact cccgcgcaac gatctcgtaa tggctggtct tgccgcggat
       61 gacacaaccg agcgcgatgc aggcgcggtg ccgccctgcc cgacccacaa agccattggc
      121 caacgcaagg ctgagtgcaa gcgggatctc gagcgcgccg ggcacggaga tgcgctcata
      181 ctcgacttga ttgccgcgca actcctcggt cgcgccgcgg gcgagctcat ccgcaatatc
      241 ttcgtagaaa cgggcctcga tgatgagaac cgagctggga cctttgcgtg tcatgcaccg
      301 ggctccaagc cacgctggcc ggcgaccttc agcccgtagc cttcgaggcc gacgatcttg
      361 cgggccggcg aattcgacag cagcaccatt tcacgcacgc cgaggtcgag caggatctgc
      421 gcgccgatac cgtattccaa cagccggggt ttgctttggc cattgcccga ttggttgagg
      481 cgcgccgata tggcgttcgg ccgtgactcc cggatcaaca cgatcacacc acgtccttca
      541 cgtccgataa tgtccatggc cttgtggagc ggcgtttcgc gtttccctcc ggacgccaga
      601 aggtcgtcga gcgtgttcat tgcgtgcacg cggaccagga cgggctcacc ggaggagacg
      661 tcgcctttga cgagcgcgat atgctccgcc gggtcgacgg tcgtggcgta gaccttcgca
      721 tcgaacgggc cgccgatggc gctgtccagc gtcgtcttgg ccacctgctt cacgagatgg
      781 tcgtagcgca gacggtaggc gatgagatcg gcaatggtag cgatcttcaa gtcatggtct
      841 tgggcgaagc tgacgaggtc gggcagccgc gccatggtgc cgtcgtcgtt catgacctcg
      901 cagatgacgc cggcagggtt cagcccggcg aggcgcgcaa tatcgacggc cgcttcggta
      961 tgccctgccc ggaccaaaac cccgccatcc ttggcgagca gcggaaacac atggcccggg
     1021 gtcatgacgt cgcgatagtc cttggtggga tcgatggcga cggagacggt gtgcgaccgg
     1081 tcgtgtgcgg agatcccggt ggaaattccc tcgcgggcct cgatcgacac ggtgaaggcg
     1141 gtcgaatggc gcgaccggtt cgagcgcgac atcaactcca agcgaagttg attggtgcgc
     1201 tctgaggtca gcgcaagaca aatgagcccg cggccatgct tggccatgaa gttgatggca
     1261 tcgggggtcg ccatttgcgc agggatcacg agatcgcctt cgttctcgcg ctcctccgca
     1321 tcaaccagaa tgaacatccg gccgttgcgc gcgtcctcca ccacctcttc gatgggagag
     1381 agatattggt ggtacgaggc gcggtcggat gccttggtta cggccgtcat gactttttgt
     1441 ctttgggttg cgtcgaactg tcttcggctt gcgccaggcg cgctgcgtag cgcgcgagga
     1501 ggtcggcttc aatgtttacc agatcgccgg gctgccggtc tccccacgtc gtgtgcgtca
     1561 aagtgtaagg aatgacattg atgccaaagc gctgaccatc cacctcgtta accgtaaacg
     1621 acacgccgtt gagggcgacg gagcctttgg gcgcgatgaa gcggccgata tcgctcggcg
     1681 cctgcaaaag gaagcgtacg cagtcgccgt cggggtagcg cgagaccact ttggcccgcc
     1741 cgtcgacgtg accggtaacg atatgcccgc cgagttcctc gccaagcgcc agcgcgcgtt
     1801 ccagattgat cgcctggcca acgtcccaag ccccaagggt cgtgtgggcc atggtttcat
     1861 tcgacacgtc gaccgtaacg acggcgccct tgtctttacc cttgtcgatg tcgaccaagg
     1921 tcaggcagca accgtcgaag gcgatggatg caccctcctc caagctctta cgcttgaatg
     1981 ggcacgcgat ggtgtatgtg gctcccttcc gcgaaaagat cgtacctacg ccgctgacaa
     2041 tgcctgtgaa catgacctga cccggcgcca cgacgtcatc cggtccggtc cgaacgtacg
     2101 cgtggcagag agtgtgaaat ggccgcttgc cgccaggcga tcaaggccat cgccggcaaa
     2161 cggcgtcagc ccatcgctgc ctgcttggcc tcggccctga tagacgacaa cctcgtctac
     2221 catatccgca tcgagaaagg cgcgcgccac ggatggtccg ccctcgacca ggatgctggt
     2281 gatgccgcga tgggagagcg tctccagcgc atcctgcaca tcgatgcgcc cgtgattgtc
     2341 cacggggacc ggcacgattt ccgcgccgcg gtcgtgcagc agttcggcat tcggctggtc
     2401 caccccggct gcacagagaa gccagaccgg caccataatg tcgtcgaaga gcgttgcgtc
     2461 cggcggcgtg cgcaagcggc cgtcgagtac ggcgcggatc ggcgagcgcc acgccatgcc
     2521 cggcagacga caggtgagtg ccgggttgtc ggtgaccacg gtgccacggc cgacaagaat
     2581 gacgtcgtga cgggcgcgca agagatgcgc atgggcgcgc gcttccggac ccgtcaccca
     2641 gacaggcttt ccatcaccct ccggcacgag cccgtcggcg ccaacggcaa gttttagtgt
     2701 cacggccggc cggccttcgg cgacgcgcat caaatggccc agcgtcacgc gcgcgacctc
     2761 ctcaggcaga accccttcgg tgacctcgat gccggccgcg cgcaagcgct cgataccggc
     2821 ccctgcggtg cgcggatcgg gatcgcgcaa gccgattaca atgtgctttg gcttcgcctc
     2881 gagcagcgcg tccgtgcacg ccatgcctcg cacgctttca tgcgcgcacg gctccagtgt
     2941 cacgtagacc gtactgcctt ctgctttggc gccggcatgg ttcagcgcaa tggcttcggc
     3001 atggggccgt ccgcccggcg ccgtccagcc gcgtcctgcg atttcgcccg atggcgtcac
     3061 cacaaccgcg ccgacggccg ggttcggcca ggcaagcccc agaccccgtg acgcaagccg
     3121 cagcgcttgc gccatgcggt actcgtcttg cggctgatgg tggtcctgcg ggctcatgcg
     3181 ttgacagcct gcctaatcgt cttcggccgt gggttcgtcg atgtcgacat cggcgtcctc
     3241 accttcggtc ccggcaagct cgccgaggag gttctcgaaa tccttggcct cacgaaaatt
     3301 cttgtagacg gatgcgaagc gcacataggc cacgtcgtcg aggcccttca acgcttccat
     3361 caccaagcgc ccaacggtct gcgagcggat gtcactttcg cccatgttct caagacggcg
     3421 gacgatcccg ctgaccatgt gttcggtgcg ctcgggctca acgggccgtt tgcgcaacgc
     3481 gatctgtacc gaacgcatca gcttgtcgcg gtcgaaggga atgcgccgac cgcttcgctt
     3541 gatcacggtg agttcgcgaa gctgcacgcg ctcgaaagtg gtgaagcgac cgccgcaatc
     3601 ggagcagatg cggcggcgcc tgattgtggt gtggtcctcg cttgggcgag aatccttcac
     3661 ttgcgtgttg gtgctgccgc aatacgggca ccgcatgggc gaccgcctcc cctagttcac
     3721 tcgtcacacg agaggcttac tcgtagatcg gatgacgatc gcagagctcg atcgcgcgct
     3781 gctttacgga ttcctcgaca gcactattgc cttcatcgcc gttggcggcg agaccgtcga
     3841 gcacttcgag gatcattccg ccgacggaga tgaattccgg gacaccgaag ccacgcgtcg
     3901 tgccagccgg cgaaccaagg cggatgcctg aggtcaccat cggcttctcg ggatcgaacg
     3961 ggacgccgtt cttattgcag gtgatgtgtg cgcggccgag agcaatctca gcagcctttc
     4021 ccgtcagctt cttcggccgc aggtccacca gtatcagatg cgtgtcggtg ccgccggaga
     4081 cgagcgcgag cccgccgttt accagtgtct cgccgagggc cttggcgttg tcgatgactg
     4141 actgggcgta aagcttgaag gacggctgca gcgcctcgcc gaaggcgacg gccttgccgg
     4201 cgatcacgtg catcagagga ccgccttgca ggcccgggaa caccgccgaa ttgatcttct
     4261 tgccgatgtc ctcgttgttg gaaaccacca tgcccgagcg cgggccgcgc agcgtcttat
     4321 gcgtggtggt ggtgcagacg tcggcgtatg gcagcgggct cggatgcagg ccagccgcga
     4381 cgagaccggc gaagtgcgcc atgtcgacca gaagataggc gccgaccgca tcggcgatct
     4441 cgcggaagcg cttgaagtcg atctggcggg gataggctga accgccagcg atgatgagcg
     4501 cgggcttatg ttcgttggcc agacgctcaa cctcgtcata gtcgatgagg ttggtgttct
     4561 gtgtcacgcc atactgcacg gcgttgaacc acttgccgga caggttcggg ggcgcgccgt
     4621 gggtcaagtg accaccggcg gcgaggctca tgccaagaac cgtatcgccc ggctttataa
     4681 gcgctagcat gaccgcctgg tttgcctgcg agcccgagtg cgtctgcacg ttggcgtagt
     4741 tgcaatcgaa caagcgcttg gcgcgatcga tggcgagctg ctcgaccacg tcgacgtatt
     4801 ggcaaccgcc gtaataccgg cggcccggat agccttcggc atacttattt gtaaggaccg
     4861 tgcccaccgc atcgatcact gcccgggaca cgatgttctc cgaggcgatc agctcgattt
     4921 cgttctgctg ccgaccgcgc tcaagctcga tggcgtccca gacttcggga tcggcctctt
     4981 ccagatgctt ggtgaaaaag gcgctttcgc gcgccgctgc gcgctgaata tcactggccg
     5041 tcatggcgaa ctgctcctcg atatcaacaa tcccgcctgg cctggcgtga ggccggcggt
     5101 tttctcctaa aacgacgcgc ttccttggca cgtttggctg gctctaccac atgtcgcgcg
     5161 gacgcgccag caacgcccca accgtagacc ggcgtgggtc agcctagagg ccgtatgcca
     5221 ggaacccaac agtctttgcg cttgttacgc caccttagag gccgaaagac ggcccggccc
     5281 cggctatccg cgtcaggctc cagccggtgt ccgattccag ggcatgactt ggagaagctt
     5341 tgccatgccg cgccagcccg ttgcgccgcc gaacaccaat cctgcgccga cgaaaccggc
     5401 gagcacgtag aagccgggcg cggcgaacgc accgagcagg acgaacagga gtacgagagt
     5461 gccagcgccg atcatgacct ggcggttaat ctcgatgggc ggactcttgt ccgcaaccac
     5521 cgggagccct gccttcttcc aggcttcaat gccgcctgtg aggctatagg cctctgattg
     5581 tgcggcgtcc ttcaagcgcc ccgcattgtt ggccgtccgc atgcctgatc ggcagtggaa
     5641 gacgatcgga cccttcacgc ccgcgaagtt cgtcgccggg cccagcggtc cgtgctgcga
     5701 gcccgggatg tgttcgcgtg cgcgttcgtc cgcaccacgg atgtcgacga gtgtcgcgcc
     5761 ttgatcgatc agtttcttgg cgtcttgcgg ggaaatgctg ggaaggctca tcggacgctc
     5821 cataggggtg ttgttgttgc gatcagcctg cgcggctcaa tcgtcgctgc aatagagctt
     5881 gtggaggtag gcgagcagga gttcgatacg cggctcggca atgcggtacc aaagcgtctg
     5941 tccctcacgg cggaacgcga cgagcccctc gtcgcgcatc ttggcgagat gctgggaagt
     6001 tgccgattga gacagaccga tcgcctcggc caacgcaccg acgcttccct cgccccactc
     6061 caccagcttg cacagcacca tcagacggcg ctcgttggcg agcgcccgta acatggtggc
     6121 gaccatggtg gcgttggctt cgaatgtcgc gagatcgtcg ggcgcacgcg gcatgctctt
     6181 gaccctttgt ctattagctt atactaattt agggtcttct aatacgttag tcaacggaga
     6241 gcggcttttg gcagagcccg ccgcggcatg cgcggacgct agaagcccat ccaggctgtc
     6301 gccggataag ctctttgaag cttgctggtg tttcttgtgg cgggagccta gtaggccgaa
     6361 gcgtaggcgc ctgaggtgtc gtgactgtag atgtcagggc ggaagtgcac ccgcccatcg
     6421 ggcgtcgccc aggcgctgat ataggcaaga tggacgggga tccgtttcgt gggcacgttc
     6481 acctgctgcc tattgtgctt catggcctga acctgtgaca ggttccattg cggattgccg
     6541 cgcaacgtcc aagcgaccaa cttgtcgaca ttgtgcacgc gcacgcatcc tgaactttcg
     6601 aagcgtaccg ccttgccgaa cagccccttc agcggcgtat cgtgcaggta gaccgcgtcc
     6661 ttgttcggga agtcgatctt gacgaagccg agcgagttct cctcccaagg ccgctgacgg
     6721 aagttgtagc tgtagacctc ttcgccgaac cagttgatct ggcggggatc gagttgattg
     6781 ccctgccggt cgaaggcctc catgtggtac gcggcaagca tgtcctgacc gcgttgcgcg
     6841 aactgacgcc ccttcggcac aaggtcctta tggatgatgc tgcgcggcac gtaccaatac
     6901 gggttgaact tcacctggtt gatcttgctg gacagctccg gcgtcgggcg ttcgatcttg
     6961 ccgacgacag cggcatgccg ctgtgcgacc cggccacctt cgacagcctc gacctgtgct
     7021 gcgggaatat tcacgagcac gtagcggctg ccggtccgcg gcgcgagctt ctgcaaccgc
     7081 ttcagactgc cttgcagctg cgccaagcgc acggatgccg gaacgttcag cgcgcgaatt
     7141 gttgctttgg agtcgatgac gcccgtcggc ggcaagccat ggcgcacctg gaaccgcttc
     7201 accgccgcga cgacggccgc gtcgtacctg tccggcgtgc tcatgccgac caaatcgcca
     7261 ccgatctcaa gccggcggtg cagcgcgacg atctcctgtc cgcgcgagcc gggcttcatg
     7321 gccttggcgg gaactgttgg ccaaccgcct tgcgcgacga tcgctttgta tttcttgatg
     7381 gcggccttgg tcgccgcgat gttctgcctg gccgccgtcg gactaccgag gcccggattg
     7441 acgatcatcg caacttcggg atcgttgcgg ggcagttccg atttgtggga ctcgtgtccg
     7501 gggctccggg agcgcccgcc accgaatagc ccaccgaaca aaccgcctcc gccaccgccg
     7561 ccggaattgg cctgaggcgc ttgctggctg ttgctattct tgttcttgaa gatgttgaac
     7621 ggaccggcac cggacggctg aaccgtcgcg accaggactg ccaccgcgag acccgcagcc
     7681 gcaatcgcag agccgtacct gatgatgtcc gttgatttcg gcatgccgct ccccgtcgtt
     7741 gcggccctaa ctggcttaac tataagcgct ttggggccgc cgcactactc gtttagagcc
     7801 cattgatgtc agcttggtct caccaaatat ttaaccaaga ctgagcctta ggagggttca
     7861 ggtcagccgc cggtttacct gcgcggcacg tcaccttcgg aatgctttcc ctcaaagggc
     7921 cgacgctgcg tggtcctcaa cggcagcagc tctcataatg cgaagaatgc ccgagaattg
     7981 gggcgaattt gggctgtcca gcgccctcgc ccggacgcaa aaacgcccca gtggagacat
     8041 gggtccaccg gggcgctttt tgatcttcag aagatcgtga aggcttacag ctggtagcgg
     8101 atctggtccg tccagaagcg ctcgagccgg atcagggcct tgttgagcgt ctcaaggtcg
     8161 tcggcgacga cgccgccaac ctgctccaag gagcgcagct gacgctgata caggccatcg
     8221 ataaccttgg aaacctcttt gcccttttca gtgaggctca cccgcacgga gcggcggtcg
     8281 gtggtcgaac gctggtgatg gatgtaaccc gcatcgacca gcttcttgag attgtaggac
     8341 acgttcgagc cgagatagta accgcggctg gtcagctcgc cagcggtcag ttcggcatcg
     8401 ccgatgttgt acagcagcag agcctgcacg ctgttcacat cgccgcggcc gacgcgctcg
     8461 aactcatcct tgatgacatc aagcagcaaa cggtgaagcc gttcgacaag cttgagagaa
     8521 acaagataat gccccttgag gtcttccgcc ccatcggtgc tcacatgcgt ccctatgttt
     8581 agggctacat tattcatcgc ctaccctcgt tacgttgttg tttatcccca acttgatgag
     8641 gactataggc gtgggctgct aagatgagct taagcgggac gcttaaaagt gcatgaaatt
     8701 ctagtattaa ttttcgttga tgttgttgtg tctgcgcgga ttcttatgaa atgcagttcg
     8761 gctcgataag ttttagttca ccatcaattg gtgtcgaaaa cagccgatcg atctccgcat
     8821 cgctgacatc ttcaagccgc gccggctgcc attgaggctc ccggcccttg ccattaatgc
     8881 gcgcatcgat cccttcgcgg aagtcgtgcg tgccggcaag atgcgaggcc aaccggaact
     8941 cgagcttcag ggcctcgttc aagctgagcg acttgcccac ctgcatttgc ttgaaggcga
     9001 tcttcagcga ggtcggcgac ttcttgcgaa gctcggcggc ggtctgcctc gcccaatccg
     9061 cgtcctcgcc gtcttcggca tcgagccggc gcataatctc ctcgacggag tccgccgaga
     9121 agatgcgatt gatggccggc gtctgccgca ccaactcgcc ttcgcccgga tcgcgatgca
     9181 ggccgtcgag aagccggtcg atcggatggt tgtcggcaag cgccgtcttg atgacgtcga
     9241 actgcgccga cgggatgtaa tggctaacaa gacgcaaacg gtgcgcgtcg gccgggccaa
     9301 tggttgcgcc agtcagcgcg agataggatc ccatccagcc catgaggcgc ggtagaaaat
     9361 aggtgccgcc aatgtctggc agaaagccga tgcccacttg gggcatggca aagcggtagc
     9421 cctccgctgc cacgcagtgg gtccccaata ggctgatgcc cacgccgccg ccgtaaacga
     9481 gcccattgat caggggcacg ttcggccgga tgaatttctc cagaacccaa acatggctgt
     9541 aggccgcgcg gtagaattcg cggatggtcc cgatcgcgtc tttctcgttc aacgcgctca
     9601 ggaccttcaa atcaccgccg ctacagaaca cggtgggatg ggtcgactgc atcacgaccc
     9661 catagatatt cggggccggg gcccagcgct ggtagttttc gctgagggcc gcgaacattt
     9721 cgtgtgtcaa cgcgttc
//
LOCUS       DBNHVU010000129         9996 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_122891, whole genome shotgun sequence.
ACCESSION   DBNHVU010000129 DBNHVU010000000
VERSION     DBNHVU010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 9996)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9996)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9996
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_122891"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="AC16Y7_05145"
     CDS             <1..907
                     /locus_tag="AC16Y7_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HIR5708797.1"
                     /translation="RGFLKDGKPDYSSELFRKGVKIYPLSAAANPPAMDFIKGTGTEF
                     NTVHSANYEFFEELHAVIDREPLEFLDPELRGLFSTIGIQKGEPFAPDERMKKLLTQA
                     AKLGNATTRALFWYERNQDEFLYKDSYWKRGMIGNSHEYLKDQGLGGRDIDARAQYFY
                     MATFNSPAMVWKLVGKGSQYAWGYLDENGDYLDGGKSYKLNLPGDAPAEKFMSIVVYD
                     SQTRSMLQTGQPYPNKNNKRDKMLINDDGSIDIYFGPEAPEGKEANWIQTVPNKGWFC
                     LLRLYSPTEAWFDKTWRPGEIEIVE"
     gene            complement(946..2184)
                     /locus_tag="AC16Y7_05150"
     CDS             complement(946..2184)
                     /locus_tag="AC16Y7_05150"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR5708798.1"
                     /translation="MTHSNGRRRVVVTGQGVVTPLGTGVDKFWAAIKSSACGVREVQS
                     FDTEDLYITIAGEVPDFEPRERLKSKQLLLADKYSQYAGCAASEAVAQSKLELPLSDT
                     DSYRSACIIGSGVGGLTTLEFSYKMLFKENKRATHPLTLLKAIGSSASAHVSIEYGIK
                     GPTFGVVSACSTATHSIGLVYQMIRNGLVDMGIGGAAEASLNWGATRAWQAMRVLSPD
                     GLFPFSKDRNGTVLAEGSGILVLEDYERAKERGAPILAEVMGYGMTADAADMVNPSID
                     GASTAMQMALDDSGLSPSDIDYVNAHGTATAVNDINETRAIRRVFGDVADNLSISSTK
                     SMHGHCLGAGGGIEAVAAIKAIEENYVPPTVGLNEPDPECDLDYTPNKGKERDINYAM
                     SNSFAFGGLNAVLIFGPPPA"
     gene            complement(2250..2519)
                     /locus_tag="AC16Y7_05155"
     CDS             complement(2250..2519)
                     /locus_tag="AC16Y7_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR5708799.1"
                     /translation="MDEVATKIIEILRKNMKDPSKPVTLETPLSELEIESLDLAVIVF
                     DIEDTFGIEIPYNANEEVEAFATVGSVVDRVKGIIAESKASGAAA"
     gene            2874..4580
                     /locus_tag="AC16Y7_05160"
     CDS             2874..4580
                     /locus_tag="AC16Y7_05160"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004467 - long-chain fatty acid-CoA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HIR5708800.1"
                     /translation="MASKANRASTDGPQEAGSRPWLSAYPTGLSWDQDFAPTLVHTLL
                     DEAVGAYGDRPCTYFLGKRLSYAEIGALSDRAAKGLRALGVGEGVKVGLLLPNTPTFV
                     IFYFAVLKAGGTVVNFNPLYSLDEIEFQIRDSDTKIMVTLDLALLFEKVEAMLERGAL
                     EKAVVAKFTSLLPPLKAAGLKLTRRTKIARVGASRFRRQIVREKDLLANDGRYDTPVV
                     TPDSVAVLQYTGGTTGTPKGAMLTHANISINVAQVKLWGNRPQDAIDRVLGVLPLFHV
                     FAMTTVMNFGVASGIEMILMPKFELIDALRLISTQKPTVMPGVPTLFNAILHHHAIKK
                     FDLTSLDFCISGGAALPIEVKRGFEALTGSNLVEGYGLSETAPVATCNPLDKTKEGSI
                     GLPLPGTEISIRSLEDPTKEVTRGDPGEICIAGPQVMTGYWRKPDETESAFVGRYFRS
                     GDVGYMDDEGFIFIVDRIKDMINAAGFKVYPRRIEDALYEHPAVAECCVVGIPDEYRG
                     EAPKAYVRLKEGHEATAPDLMTFLRPKLSKLELPAAIEFRDELPKTMIGKLSKKELRA
                     QT"
     gene            complement(4585..5757)
                     /locus_tag="AC16Y7_05165"
     CDS             complement(4585..5757)
                     /locus_tag="AC16Y7_05165"
                     /inference="COORDINATES: protein motif:HMM:NF021438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HIR5708801.1"
                     /translation="MRHHSLTAAIGIVLAAALLPLSATSAMAADPTGYWRKAEQRSKW
                     PGKMLIHGCGSGKRYLCVKIAWVKNPVHEGKPLRDVLNKNTSLRSREIVGLQIARGLA
                     KVAGNQWKGNIYNPEDGRTYSATLTQVSRSKIILKGCMGFMFCGERVWVRTSPPPKEP
                     EPEAKPEEQIEAKAEPEATPEAASVAAAEPAASPEAATNVITPAAAASNSMGTAEMAT
                     PAAHQSTEPGYHFLNSAAAAPESANFTGNDLPSMFHMTTPLDQNAVAATEGAPTPVTQ
                     PQPQARTIEANAAPAPAPQPNPAPPQAKPAAAPKPVAAPKPIAAPTPAAAPAPAVSPE
                     PAPADVSGSTETADATDATAPVAAPSRPLTWRERRRLRRQQRLRQQTEGVLPWAGQ"
     gene            complement(5877..7205)
                     /locus_tag="AC16Y7_05170"
     CDS             complement(5877..7205)
                     /locus_tag="AC16Y7_05170"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR5708802.1"
                     /translation="MTQMPMRRVAVLGGMRIPFCRSHTAYAELSNLDMLTAALCGLAD
                     RFGLAGAHIDEVVGGAVITHAKDWNLAREAVIGSSLARTTPGITMMQACGTSLQAALG
                     LAAKIATGQIESGIAMGSDTTSDAPIVFQKKFAKRLVKASRAKSLGQRVGAFKGMAPG
                     ELAPLPPDTREPRTGLGMGEHCELMAKEWGISRQDQDILAFESHCKAAAAYDEGFMDD
                     LVVPNAGLFRDNNIREDASLEKLAELKPVFDKSGGTITAGNSTPLTDGAATVLLASEK
                     WAKSKEIPIQAYLILGRTSAVDYVEGEGLLMAPTVAVSELLDQAGLALQEFDYYEIHE
                     AFAAQVLATLKAWESEAYCRDRLGRNAPLGTIDQAKMNVKGSSIAFGHPFAATGARIL
                     SVLAKLLHSGCGQRGLISVCTAGGMGVAAILEGVEAQGTGGSEAKEMGAA"
     gene            complement(7222..9222)
                     /locus_tag="AC16Y7_05175"
     CDS             complement(7222..9222)
                     /locus_tag="AC16Y7_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HIR5708803.1"
                     /translation="MQITLPDLKDWTFSVDLEGIAWAIIDRQGESMNSLGRRAIEELG
                     EVVEALEQVQAGEVRGLVLMSGKDSGFVAGADIREFEGFDTEEKVRDVVKQTIELFDR
                     IEALPMPVVVAIHGYCLGGGLELALACDWRIADREEGTRLGFPEVKLGIFPGLNGTVR
                     SIRVAGPMDAMTAMLTGKMLRPGAARAMGLVDQLVATRHNLRWAARKAVLQKRRSKGA
                     PWWKKLMAKQPVRGMLAKQMRAKTAEKVREDHYPAPFRLIDLFERHGDDLAAMKRAET
                     EMFAPLMASETSRNLRRVFGLSEMLKDAAPKDGFRPRKVHVIGAGTMGADIAAWCVVS
                     GMQASLQDLDQGQIEKGIHRAKKLFKKRLRGKTAFDSGVARLIADPGGKQIKHADVII
                     EAIIEKLDVKQKLFADLETKAKPGAVLATNTSSLKLEDIAKPLKDPGRLVGLHFFNPV
                     AQLPLVEVVHGEGTREEEVGKACRFVTAINKLPLVVKSCSGFLVNRVLAPYMMEAVRL
                     YQDGQPREKIDQAALKFGMPMGPMELMDMVGLDICNKVGEELNLAPKTDGASDNVLAS
                     LVNQGKLGKKTGEGFYVWEDGKPKRDAEATFDDAELARLGRELVQPLLDEAARAEADG
                     IVKDADHVDAGVIFGTGFAPFRGGPLHYRKSQDANAAGKAAA"
     gene            complement(9232..>9996)
                     /locus_tag="AC16Y7_05180"
     CDS             complement(9232..>9996)
                     /locus_tag="AC16Y7_05180"
                     /inference="COORDINATES: protein motif:HMM:NF020876.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1974 domain-containing protein"
                     /protein_id="HIR5708804.1"
                     /translation="QSPGDVTYWWRQLVRAARSFALVADVTVALLGGGLKVKQKIAGR
                     MADALSELYLLSAILKRYEDDGRQAADRKVVDYCAQNCLHRFDVALRGVIDNFPARWA
                     AWAMKPLVLTYGSRAPASDKHGKDIVRLALKPGAFRDRLTREIYAPDDPADPIGLLEA
                     TLVKAIACEEAEKKLERAIRKGEVRRLYNTDWIKDAEEKGILSADEARDLASLRDMTA
                     RVIAVDDFAAVDLKHEDKTLQSGDADPSARAHIAAE"
BASE COUNT         1855 a         3161 c         3047 g         1933 t
ORIGIN      
        1 gcgcgggttc ctgaaggacg gcaagccgga ttactccagc gagttgttca gaaagggcgt
       61 caagatctat ccactcagcg cagctgccaa tccaccggcg atggacttca tcaagggaac
      121 cggaaccgaa ttcaataccg ttcactctgc gaactacgag ttcttcgaag aacttcatgc
      181 cgtgatcgat cgcgagccgc ttgaatttct ggaccctgag cttcgtggtc tcttctcgac
      241 cattggcatc cagaagggtg aaccgttcgc gccagacgaa aggatgaaga aacttctgac
      301 gcaggccgcg aaactgggca atgcaacgac acgcgccctg ttttggtacg aacgcaatca
      361 ggacgagttc ctctacaagg acagctattg gaagcgcggc atgatcggca attcccatga
      421 gtacctgaag gaccagggac tgggaggccg cgatatcgat gcaagagcgc aatatttcta
      481 catggcgacg ttcaattcgc cggccatggt ctggaagttg gtcggaaagg gctcccaata
      541 tgcgtggggc tacctggacg aaaatggcga ttatctggac ggcggtaagt catacaagct
      601 gaaccttcca ggggatgcgc ccgcggagaa gttcatgtcc atcgtggtct acgattcgca
      661 gacccgctcc atgttgcaga ccggtcagcc ctacccgaac aagaacaaca agcgcgacaa
      721 gatgctgatc aacgatgacg gttcgatcga catctacttc gggccggaag cgcctgaagg
      781 aaaggaagcg aactggattc aaaccgtccc caacaaaggg tggttttgcc tgttacgcct
      841 ctacagtcca accgaggcat ggttcgacaa gacctggcga ccaggcgaga tcgagatcgt
      901 cgagtaggga agagcgaagg gctaggcgat caggccgagt gcgatttacg ccggcggcgg
      961 gccgaatata agcacggcgt tgaggccgcc aaaggcgaag ctgttcgaca tcgcgtagtt
     1021 gatgtcgcgc tctttgccct tgttcggcgt gtagtctaga tcgcattccg gatccggctc
     1081 gttgagcccc acggtcggcg gcacgtagtt ctcctcgatc gccttgatcg cggcgaccgc
     1141 ttcgatgcca ccaccggcgc cgaggcaatg gccgtgcatc gacttcgtcg aggagatgga
     1201 cagattgtcg gcgacatcgc cgaacacgcg tctgatggcg cgggtctcgt tgatgtcgtt
     1261 caccgccgtc gccgtgccat gggcgttcac gtagtcgatg tcggacgggc taagcccgct
     1321 gtcgtccagc gccatctgca tggccgtgga ggcgccgtca atggatgggt tgaccatgtc
     1381 tgcggcatcc gccgtcatgc catagcccat gacctctgcc aggatcggcg cgccgcgctc
     1441 tttggcgcgc tcatagtcct ccagcaccag aataccggag ccctcggcta ggaccgtgcc
     1501 gttgcggtcc ttcgagaagg gaaacagccc atcggggctc agcacacgca tggcctgcca
     1561 ggcgcgcgtg gcgccccagt tcagcgaggc ttccgcggca ccgccgatac ccatatcgac
     1621 caggccgttc cggatcatct ggtagacgag gccgatggag tgcgtggccg tcgagcaggc
     1681 gctgaccacg ccgaaggtcg gacccttgat gccgtattcg atgctcacat gagccgaggc
     1741 ggacgagccg atggccttca ggagcgtcag gggatgggtc gcgcgcttgt tctccttgaa
     1801 gagcatcttg taggagaact cgagcgtggt gaggccaccg acgccggagc cgataatgca
     1861 ggccgagcga tagctgtcgg tgtcgctcag cggcagctcc agcttcgact gggccacggc
     1921 ttctgacgcg gcacagcccg catattggga atacttgtcg gccagcagca gctgcttgct
     1981 cttcaagcgc tcccgcggct cgaagtcagg cacctcgccg gcaatcgtga tgtacaggtc
     2041 ttcggtatcg aagctctgga cctcgcgaac accgcaagcg ctgctcttga ttgcagccca
     2101 gaacttgtcc acgcccgtac ccaaaggcgt aaccacgccc tggccagtga ccacgacgcg
     2161 gcggcgtccg ttagagtgtg tcatgtagtg ttaagccgta ctccaagcct agtcgacgtc
     2221 ccgggaagga cccgagcgga ctggccggtt taagctgctg cgcccgaggc cttcgattct
     2281 gcgataatgc ccttcacgcg atcgaccaca gagccgaccg tcgcgaacgc ttccacttct
     2341 tcgttcgcat tataagggat ctcaatacca aaggtatcct caatatcgaa tacgatcacc
     2401 gccagatcga gggattcgat ctcaagctcg ctaagcgggg tttcgagcgt aacgggcttg
     2461 gacggatcct tcatgttctt gcgcagaatc tcgatgatct tggtggccac ctcgtccata
     2521 tcactctccc tgccccgtta aggggtccgt atctaatggt ccctggctaa aacctagcta
     2581 gtcctagggc aagtcaggct ttggaataga cttaaaaact agccgagtct acccgtgccc
     2641 aactcactgg attcgccatt actataggtt ctaatcgcta acaaacttgg cgtttttgcg
     2701 cctggccgcg ccttggtttc cgcatattcc gcaaggggtg tgccctgttt cgggcctgaa
     2761 tatcaacccc gtgctacaat tatactagag gaacgggctt gcggccaatt gcctccgtca
     2821 gacggccttt cacccgttaa ccagcggcaa acagggcaaa aaaccgaaga tctatggcta
     2881 gtaaggcgaa tcgggcatcc acggacggtc ctcaagaggc tggaagtcgc ccttggctgt
     2941 ccgcctatcc gaccggcctc tcctgggatc aggactttgc ccccaccctc gtccacaccc
     3001 tgctcgatga ggcggtgggc gcatacggcg accggccgtg cacctatttc ttggggaaac
     3061 gcctgagcta cgcggagatc ggcgcgctct ccgaccgcgc cgccaagggc ttgcgggcgc
     3121 tcggcgtcgg cgaaggggtg aaggtggggc tgctgctgcc caatacgccc accttcgtga
     3181 tcttctattt cgcagtgctc aaggccggcg gcacggttgt caacttcaat ccgctctaca
     3241 gcctggacga gatcgagttt cagatccgcg acagcgacac caagatcatg gtgacgctcg
     3301 acctggcgct tctgttcgaa aaggtcgagg ccatgcttga gcgcggcgcg ctggagaagg
     3361 ccgtggtggc gaagttcacc tcactgctgc cgccgctgaa ggcggccggc ctcaagctca
     3421 cgcggcggac gaagatcgcc agggtcggcg cctcgcgctt ccgccgccag atcgtgcgcg
     3481 agaaggactt gctagccaat gacggccgct atgacacacc ggttgtcacg cccgattcgg
     3541 tcgccgtcct gcaatacacc ggcggcacca cgggcacgcc taagggcgca atgctgaccc
     3601 acgccaacat ctcaatcaac gtggcgcagg tgaagctatg gggaaacagg ccgcaggacg
     3661 cgatcgatcg cgtgctcggc gtgctgccgt tattccacgt ctttgccatg accacggtga
     3721 tgaacttcgg cgtcgccagc ggtatagaaa tgattctcat gccgaaattc gagctgatcg
     3781 acgctttgag gctgatcagc acgcagaagc caacggtgat gcctggcgtc ccgactttgt
     3841 tcaacgccat cctgcatcat cacgcgatca agaaattcga tctgacctcg cttgatttct
     3901 gcatctccgg cggcgcggcg cttccgatcg aggtgaagcg gggcttcgag gcgctgaccg
     3961 gcagcaactt agtcgaaggt tacggtttga gcgaaaccgc accggtcgcc acctgcaatc
     4021 cgctcgacaa gacaaaagaa ggctccatcg gcttgccgct tccgggcacc gagatcagca
     4081 tccgctcgct tgaggatccg accaaggagg tcacgcgcgg cgacccgggc gagatctgca
     4141 ttgccggacc gcaagtgatg acggggtatt ggaggaagcc ggacgaaacg gagtccgcct
     4201 tcgttgggcg ctacttccgc agcggcgacg tgggctacat ggacgacgaa ggcttcatct
     4261 ttatcgtcga ccgtatcaag gacatgatca acgccgcggg attcaaggtc tatccgcggc
     4321 gaatcgagga tgcgctctac gaacatcctg cggtcgcgga gtgctgcgtg gtggggattc
     4381 cggatgagta ccggggcgaa gcacccaagg cctatgtgcg cctaaaggaa gggcacgaag
     4441 cgactgctcc ggatctcatg acgttcttgc gcccgaagct atccaagctc gagctgccgg
     4501 cggccataga gttccgcgac gagttgccga agacgatgat cggcaaactc tccaaaaaag
     4561 agctacgcgc gcagacctag agcgtcactg accggcccag ggaagcaccc cttcggtctg
     4621 ctgccgcagt ctctgctgac gccgcagcct gcgccgctcg cgccaggtca gcggccgtga
     4681 tggcgcggcg acaggggcag tggcgtcggt ggcatcggcg gtctcggtcg agccgctgac
     4741 gtcggccggg gccggctcag gagacactgc gggcgccggg gcagctgcgg gcgtaggcgc
     4801 cgcaatgggc ttgggcgcgg cgacaggctt gggcgccgct gcgggcttcg cttgtggcgg
     4861 cgccggattc ggttgcggtg ccggcgcagg tgccgcattc gcctcaatcg tccgcgcctg
     4921 cggctggggc tgcgtcacgg gcgtcggtgc gccttcagtc gcggcaacgg cgttctgatc
     4981 gagcggcgtc gtcatatgga acatgctcgg caggtcgtta cccgtgaagt tggcggattc
     5041 cggcgcggca gcggcgctat tcaagaagtg atagccgggc tccgtgctct gatgcgcggc
     5101 gggcgtggcc atctccgctg tacccattga gttcgatgca gccgccgctg gcgtgatgac
     5161 gttcgttgcc gcctcgggcg atgccgctgg ctccgccgcg gctacagacg cagcctctgg
     5221 cgtggcttcg ggctcggctt tagcctcgat ctgctcctca ggcttggcct cgggctcggg
     5281 ctccttcggc ggcggcgagg tcctcaccca gacgcgctca ccgcaaaaca taaaccccat
     5341 acagcccttg agaatgatct tgctgcgcga gacttgcgtc agcgtcgcgg agtaggtgcg
     5401 gccgtcctcc gggttgtaga tgttgccctt ccactgattg ccggcgacct tcgcaagacc
     5461 tctggcgatc tgcagcccga cgatctcacg gctgcgcaac gacgtgttct tgttgagaac
     5521 gtcgcggagc ggcttgccct cgtgtacggg attcttcacc caggcgatct tgacgcagag
     5581 atagcgcttc ccggagccgc agccgtggat cagcatcttg ccgggccact tgctgcgctg
     5641 ctcggctttt cgccaatagc cagtgggatc agcggccatg gcgctggttg cactcaaggg
     5701 aagaagagcg gcggcaagaa cgatgccgat cgccgcagta agagaatgat gccgcattag
     5761 cgcacctccg tcatggtcgg gccgccggcg caccgtgcac ctcggcgaaa atggcttctg
     5821 ccccctgtcc ctgacgggtc tcgttggacc caattcctga tccggcgata gagcacttat
     5881 gctgcgccca tttccttagc ttccgaaccg cctgtgcctt gcgcctcgac cccttccagg
     5941 atcgccgcaa cgcccatacc gccagcggta cacaccgaga tcaaacctct ctggccgcac
     6001 ccactatgta ggagtttagc caaaactgac aagattcgcg cacccgtggc ggcaaaggga
     6061 tggccaaagg cgatcgagga gcctttgacg ttcatcttgg cctggtcgat cgtgcccaag
     6121 ggcgcgttcc gccccagacg gtcccgacaa taggcttctg attcccatgc cttcaaagta
     6181 gccaacactt gagcagcgaa tgcctcgtga atctcataat aatcaaactc ttgaagcgca
     6241 agtccggcct gatcgagcag ttcgctgaca gccacggtcg gcgccatcaa gagaccttca
     6301 ccttccacat agtctacggc agaagtgcga cccaaaatta aataagcctg aatcggaatt
     6361 tccttggatt tcgcccattt ttcgctcgcc agcagcaccg tggcggcccc atccgttaac
     6421 ggggtcgagt tgccggcggt aatcgtgccc cctgatttgt cgaaaacggg cttcagttcg
     6481 gccagcttct ccagggaagc atcctcccgg atattgttgt cacggaagag cccggcgtta
     6541 gggacgacga ggtcgtccat gaagccttcg tcgtaggccg cggcggcctt gcagtggctt
     6601 tcgaaggcga ggatgtcctg atcctggcgg gagatgcccc attccttggc catcagctcg
     6661 cagtgttcgc ccatgccgag gcctgtgcgc ggctcgcggg tatccggcgg cagcggcgcg
     6721 agctcgccgg gcgccatgcc cttaaacgcc ccgacacgct ggcccaggct tttggcgcgc
     6781 gaggccttga ccaggcgctt ggcgaacttc ttctggaaaa cgatgggcgc atcgctcgtc
     6841 gtatccgagc ccatggcgat gccggactcg atctggcccg tggcgatctt ggcggcaagt
     6901 cccagcgccg cctgaaggct cgtgccgcag gcctgcatca tggtgatgcc cggcgtggtg
     6961 cgcgccaacg agctgccaat caccgcctcg cgggccaagt tccagtcctt ggcatgggta
     7021 atcaccgcac cgccgaccac ctcgtcgata tgtgcgccag ccaagccgaa gcgatcggcg
     7081 agcccgcata gcgccgccgt cagcatgtcg aggttggaaa gctcggcgta agcggtgtgc
     7141 gagcggcaga aggggattcg catcccaccc agcaccgcga cacgccgcat cggcatttgc
     7201 gtcatgacgt cctttccagc ctcatgctgc ggccttgccg gcagcgttcg cgtcttggct
     7261 cttccggtag tgcagcggac cgccgcggaa cggcgcgaaa cccgtgccga agatcacacc
     7321 cgcatcgacg tggtcggcgt ccttgacgat tccgtccgcc tcggcgcgcg cggcttcgtc
     7381 gagcagaggc tggacgagct cgcggccgag ccgcgccaat tcggcgtcgt caaaggttgc
     7441 ctccgcgtcg cgcttcggct tgccgtcttc ccacacatag aagccctcgc ccgtcttctt
     7501 gccgagcttg ccctgattca ccagcgaggc aagcacgttg tcggacgcac cgtccgtctt
     7561 cggtgcaagg ttcagctcct cgccgacctt gttgcagatg tcgagaccga ccatgtccat
     7621 cagctccatc ggacccatgg gcatgccgaa cttgagcgcg gcttgatcga tcttctcgcg
     7681 tggctgtccg tcttgataga gccgcacggc ctccatcatg tagggcgcga gcacgcggtt
     7741 gaccaaaaag cccgaacagc tcttcacgac taaaggcagc ttgttgatcg cggtgacgaa
     7801 gcggcaggcc ttgccgacct cctcctcgcg cgtcccttcg ccgtgcacga cctcgaccaa
     7861 gggcagctgc gcgacggggt tgaaaaaatg aagtccgacc aggcgccccg gatccttcag
     7921 cggcttggca atgtcctcca acttgagcga tgaagtgttt gtcgccagca ccgcgccggg
     7981 ctttgccttg gtttccaggt cggcgaagag tttctgcttc acgtcgagct tttcgatgat
     8041 cgcttcgatg atcacatcgg catgcttgat ctgcttgccg cccgggtccg cgatcaggcg
     8101 cgcaacgccg gagtcgaacg ccgtcttgcc gcgcaagcgc ttcttgaaga gcttcttggc
     8161 gcgatggatg cccttttcga tttggccttg gtcgaggtcc tgcaagctcg cctgcatgcc
     8221 ggagacgacg caccaggcgg cgatgtccgc gcccatggtg ccggcgccga tgacgtggac
     8281 tttacgcggg cggaagccgt ccttcggggc ggcatccttc agcatctccg acagcccgaa
     8341 cacgcggcgc aagtttcgcg aggtctcgct cgccatcagc ggcgcgaaca tctccgtctc
     8401 ggcgcgcttc atggcggcca gatcatcgcc atggcgctcg aagagatcga tcaggcggaa
     8461 gggcgccggg taatggtcct cgcgcacctt ctccgccgtc ttggcgcgca tctgtttggc
     8521 cagcatgccg cgcaccggct gcttggccat gagcttcttc caccagggcg cacccttcga
     8581 ccggcgcttc tgcaacacgg ccttgcgcgc ggcccagcgc agattgtggc gcgtggctac
     8641 aagttgatcg acgaggccca tggcccgtgc cgcgcccggc cgcagcatct tgcccgtcag
     8701 catggcggtc atcgcgtcca tgggacctgc cacccgaatg gagcggacgg tgccgttgag
     8761 ccctggaaag atgccgagct tcacttcggg gaagccgagc ctggtcccct cctcgcggtc
     8821 ggcgatgcgc cagtcgcagg ccaaggctag ctccagcccc ccgccaaggc agtagccatg
     8881 aatcgcgacg accacgggca ttggcaaagc ttcgatgcgg tcgaacagct cgatcgtctg
     8941 cttgacgacg tctctgacct tctcctcggt atcgaagccc tcgaactcac ggatgtcggc
     9001 cccggcgaca aagcctgagt ccttgccgct catgagcacg agaccccgga cctcgccggc
     9061 ctgcacctgc tccagcgcct cgacgacctc gccgagttcc tcgatggccc tacggccaag
     9121 tgagttcatg ctttcgcctt ggcgatcgat gatcgcccaa gcgatgcctt cgaggtcgac
     9181 ggaaaaggtc cagtctttta ggtcgggaag cgtaatctgc atgatccagt cttactcggc
     9241 ggcgatatgc gcgcgggcgg acggatccgc gtcgccgctt tgcagggtct tatcttcgtg
     9301 cttcagatcg acagcggcaa aatcgtcgac ggcaatgaca cgggcggtca tgtcgcgcaa
     9361 gctggcaagg tcgcgcgctt cgtctgcgct caagataccc ttctcctcgg cgtccttgat
     9421 ccaatcggtg ttatagaggc gcctcacctc accctttcga atggcgcgtt ccagcttctt
     9481 ctcggcctcc tcgcaggcga tcgctttgac aagcgtcgct tcgagaagcc caatcggatc
     9541 ggcaggatcg tcgggggcgt agatctcccg ggtcaaccgg tcacggaagg cgccgggctt
     9601 cagggcgagc cgtacgatgt ccttgccgtg tttgtcgctc gctggcgcac gcgacccata
     9661 agtcagcacc agcggcttca ttgcccaagc cgcccagcgc gcggggaagt tgtcgatcac
     9721 gccgcgtaaa gcgacatcga accggtgcag acaattctgc gcgcagtagt cgaccacctt
     9781 gcggtcagcc gcttggcgcc cgtcgtcttc gtagcgtttg aggatcgcag acagcagata
     9841 gagctcagac agcgcgtccg ccatgcggcc ggcgatcttc tgcttcactt tgagaccgcc
     9901 gccgagaagc gccacggtaa catcggcgac gagcgcgaag gaccgcgcag cgcgcaccaa
     9961 ttgccgccac caataggtca catcgccggg actctg
//
LOCUS       DBNHVU010000130         8920 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_717213, whole genome shotgun sequence.
ACCESSION   DBNHVU010000130 DBNHVU010000000
VERSION     DBNHVU010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8920)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8920)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8920
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_717213"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /gene="ccsA"
                     /locus_tag="AC16Y7_05185"
     CDS             complement(<1..438)
                     /gene="ccsA"
                     /locus_tag="AC16Y7_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HIR5708805.1"
                     /translation="MSPMISLANPTRFLQLVDAVVPWLSALVIGLLGIGLYLAFFAAP
                     PDYQQGETVRIMFIHVPAAWVAMGCYAVIAIAALGTLIWRHPLADVAAKAAAPIGATF
                     TFIVLVTGSLWGKPMWGTYWVWDARLTSELILLFLYLGIIGLRA"
     gene            complement(472..1140)
                     /gene="ccmB"
                     /locus_tag="AC16Y7_05190"
     CDS             complement(472..1140)
                     /gene="ccmB"
                     /locus_tag="AC16Y7_05190"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HIR5708806.1"
                     /translation="MNASLALLVRDVRLAWREGGAISVALGFYLVVVAIAPFGLGPDL
                     NLLARIAPGMLWVALLLAALLSADRIFHNDYEDGTLDVLTSGPLPLPLVAGIKALAHW
                     ITTGVPLALLAPVLGLLLNLPLDAMPLLVLAMLAGTPAVSFIAGIGASLTLGLRRSGL
                     LLALLVLPLYVPILIFGVSTVSAAVVGPGSPWPPFLMLCALSLASIVLAPFAAAAALR
                     TALR"
     gene            complement(1137..1766)
                     /gene="ccmA"
                     /locus_tag="AC16Y7_05195"
     CDS             complement(1137..1766)
                     /gene="ccmA"
                     /locus_tag="AC16Y7_05195"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HIR5708807.1"
                     /translation="MPSPCSVSLEASAIACERGQRTLFEGLSFALAPGEALLVRGPNG
                     AGKTSLLRQLAGLLPLAAGTLSAPGADDDTPLSELCHYVGHLNAAKSALTVEENLAFW
                     VDFLGDGAGDLDGALGSFGLEDLAKLPAGLLSAGQKRKLALSRLFAASRPIWLLDEPS
                     VSLDTAAVKHLDAAIRAHLKGGGIAVVSTHVPLKTKFAHQLTLGLEEAA"
     gene            2003..4702
                     /gene="acnA"
                     /locus_tag="AC16Y7_05200"
     CDS             2003..4702
                     /gene="acnA"
                     /locus_tag="AC16Y7_05200"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007067244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIR5708808.1"
                     /translation="MTSAVSTSLDSFKCRRTLKVGKREYDYFDLKKAEAAGLDGIGRL
                     PFSLKVLLENLLRHEDGRSVTDDDIRAVADWLKDKRSDREIAFRPARVLMQDFTGVPA
                     VVDLAAMRDAVTKLGGDPHKINPLAPVDLVIDHSVMVDAFGTGAAFQFNVDKEYLRNS
                     ERYAFLRWGAGAFDNFRVVPPGTGICHQVNLEYLAQTVWTKKENGTRLAFPDTLVGTD
                     SHTTMVNGLSVLGWGVGGIEAEAAMLGQPISMLIPEVVGFRLTGTLREGVTATDLVLT
                     VTEMLRRKGVVGKFVEFFGTGLDNLPLEDRATIANMAPEYGATCGFFPVDKETLDYLE
                     ATARKDSRVELVTAYAKAQGLYRKDKTPDPVFTDTLALDLGDVVPSIAGPKRPQDRVP
                     LTRAAPAFAEVLDKEYGKAAEADKRVPVEGKNFDLGHGDVVIAAITSCTNTSNPYVMV
                     AAGVLARNAVKEGLSVKPWVKTSLAPGSQVVTDYLDRAGLQGDLDKLGFNLVGYGCTT
                     CIGNSGPLPEEISKTIHDNGLAVASVLSGNRNFEGRVNHDVRANYLASPPLVVAYALA
                     GSVTKDLETQPLGVGKNGPVYLKDIWPSSKEVAKLVRKAVKKSMFKERYGDVFRGDPE
                     WQAIKVDEGLTYDWDTKSTYVQEPPYFSDMSAEPAALTDIDGARILGLFLDSITTDHI
                     SPAGAIKKDSPAGHYLTENGVAVHDFNSYGSRRGNHEVMMRGTFANVRIKNQMVPGIE
                     GGVTVHYPSEERMAIYDAAMRYQDEGMPLVVFAGKEYGTGSSRDWAAKGTRLLGVRAV
                     IAQSFERIHRSNLIGMGVLPLVFAEDMSWEKLGLTGSETVSLKGLNDLKPGQWVEADI
                     VYPGGETRAVPLQVRIDTFDELDYFRNGGILHYVLRSLAKEAA"
     gene            4775..5548
                     /locus_tag="AC16Y7_05205"
     CDS             4775..5548
                     /locus_tag="AC16Y7_05205"
                     /inference="COORDINATES: protein motif:HMM:NF018473.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HIR5708809.1"
                     /translation="MRLVTLLAALVGAACTAALLALAFSVPSRAEKPKLATDTPVLIE
                     LFTSQGCSSCPPADALLNEYRDKPGVITLSFSVDYWNYLGWHDTLSSAENSERQRDYA
                     LARGDGAVYTPQVVVDGITHVNGANEAAIEMAMRQTTRRLSDVKVPMSMRAEGKSLVV
                     DIADAPETSDMREATIWLAVAKDQETVAIDRGENRGKEITYSHPVRDFTPIGMWKGEK
                     MSLRLPLKDLQTIGGDCLVAMLQVEGSGPVLGAAAYERD"
     gene            5841..6251
                     /locus_tag="AC16Y7_05210"
     CDS             5841..6251
                     /locus_tag="AC16Y7_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HIR5708810.1"
                     /translation="MTLPLQEARLSETEPIVVPLAFRAGGLQSPSEGGARRDGISFDR
                     RELSQILNVYVRKVASGEWRDYAIDTLKEKAVFSVFRRSSEMPLYRIEKTPRLARRQG
                     AYTVIAATGLILKRGHDLARVLRALDKGLRLVEA"
     gene            complement(6513..7262)
                     /locus_tag="AC16Y7_05215"
     CDS             complement(6513..7262)
                     /locus_tag="AC16Y7_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HIR5708811.1"
                     /translation="MAELDRRYGQSVGRADAGAIDQGLRAYMIRVYNYMASGVAITGV
                     VAYAIYAMAVQQGAGGLELTAFGNFMFASGFKWVVIFAPLAMVFFLSMRIGSMSLGTA
                     QLSFWVFSALMGASISSIFLVYAGESIARVFFITAASFGALSLWGYTTSKDLSGWGSF
                     LFMGLIGIIIASLVNLFIGSTALQFAVSVIGVLVFAGLTAYDTQQIKEMYSANDDGSM
                     SGRKAVMGALRLYLDFINLFMMLLQLFGDRR"
     gene            complement(7398..>8920)
                     /locus_tag="AC16Y7_05220"
     CDS             complement(7398..>8920)
                     /locus_tag="AC16Y7_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5708812.1"
                     /translation="YEDTLPLRLAVEPHPMPLIVAALAGLLTMVLFVLWPLGRASRIS
                     PAVLMRAHLTEERERSTTAFALGSAAAGLALFVLAIAASEERLVTAAISLGILAAFGL
                     LLGFGLLVQRLAARYRRTKPPAWGLALASIGAPGSLARSIAVSLGLGLGLLIAVALIH
                     HSLITEIESHVEVDAPAYYFLDVEPGDLATFEQTTKEIQPKAKTDNAPMLRGRIVKLN
                     GVSVDDVEVKPDARWVLAGDRGLTYTDTVPGASKVEEGEWWPKDYDGPPLVSFDGELA
                     KGLGLKLGDQITVNILGRNVEAKIASLRKIDWESLAINFVMVFSPNTLQGAPHRLVTT
                     LELPKGTDPDEEAKIIQSLAERFPLVTAIKIGDLVDAAKAMLEKLMGAIQATSGFTLL
                     IGAAVLAGAVSAGQQRRKYLAVLYKTLGATRGLIVKAELLEFGVLGLATSLLAILIAT
                     ITAWALCTFAFDIEFKFAPLAAAATIGLALALVLIVGAFTTWRVLSVKAAPYLRSE"
BASE COUNT         1742 a         2693 c         2983 g         1502 t
ORIGIN      
        1 tgcgcgaagc ccgatgatcc cgaggtagag gaacagcagg atcagctccg aggtgagtcg
       61 ggcatcccac acccagtagg tgccccacat cggcttgccc cacagcgacc cggtaacgag
      121 cacgataaag gtgaaggtgg cgccgatggg tgccgcggcc ttggcggcaa cgtcggccag
      181 cgggtggcgc cagatcagcg tgccgagcgc cgcgatggcg atcactgcgt agcagcccat
      241 ggcgacccac gcggcgggca cgtggatgaa catgatgcgc accgtttcgc cctgctgata
      301 gtcgggcggc gccgcgaaga acgcgaggta gagcccaatt ccgagaaggc cgatgacaag
      361 ggccgagagc cagggcacga ccgcatcgac gagttgcaga aagcgcgttg ggttggcgag
      421 gctgatcatg gggctcatct agaggctttg gctgccatgc gcaaatgggg tctatcgaag
      481 cgcggtgcgt aacgccgccg cggccgcaaa cggtgcgagc acgatgctgg caaggctcag
      541 cgcgcagagc atgaggaagg gcggccaggg ggaaccgggc ccgacaaccg ccgccgagac
      601 ggtggacacg ccgaagatca ggatcggcac gtaaagcggt aacaccagaa gcgcaagaag
      661 caggccgctg cgccgtaagc cgagcgttag gctggcgccg atgccggcaa tgaagctgac
      721 ggcgggggtg cctgcaagca tcgccagcac cagcagcggc atggcgtcga gcggcaagtt
      781 cagaagaagc ccgagcacgg gggcgagcaa agcgagcggc acacccgtcg tgatccaatg
      841 tgcgagggcc ttgatgcctg cgacaagcgg caatggcagg gggccgcttg tgagcacatc
      901 taaggtgccg tcctcatagt cgttgtgaaa gatgcggtcg gcggacagca gcgcggccag
      961 gagcagcgcc acccacaaca tgccgggcgc gatgcgggcc agcagattga gatcggggcc
     1021 gagcccgaac ggggcgatgg cgacgaccac gagatagaag ccgagcgcaa cgctgattgc
     1081 gccgccttcg cgccaggcta gccgcacatc gcggacgaga agggcgaggc ttgcgttcat
     1141 gctgcctcct cgagcccgag ggtgagttgg tgcgcgaatt tcgttttcag cggcacatgg
     1201 gtggagacga cggcgatgcc accccctttg agatgagcgc ggatggcggc gtcgaggtgc
     1261 ttcaccgcgg cggtgtcgag cgagacggac ggctcgtcga gcagccagat ggggcgggat
     1321 gcggcgaaca gccgtgacag ggcgagcttt cgtttctgcc ctgccgagag caggccggcg
     1381 gggagcttgg cgaggtcttc aaggccgaat gagccaagcg cgccgtcgag atcgcccgca
     1441 ccgtcgccca agaagtccac ccagaaggcg aggttctcct caaccgtgag ggcgctcttg
     1501 gccgcattga ggtggcccac atagtggcaa agctcggaga ggggcgtgtc gtcatccgcc
     1561 ccgggcgccg aaagtgtccc cgccgctagc ggcagcaggc cggccaactg gcgcaagagg
     1621 ctcgttttcc cggcgccgtt ggggccacgc accagcagcg cctcgccggg ggcaagcgcg
     1681 aagctcaacc cctcgaacag cgtccgctgc ccgcgctcgc aggcgatggc actggcttcc
     1741 aggctgacag agcagggcga tggcatggga tcttttcgct taaagtagag gcgattggcc
     1801 tcgttcaggg gttcagacgc gtctcttcgc gtcattgtct cgttgccgca agggctttta
     1861 tagtgggcca agagcacgta accaaaaggc caggacgccc gggcgcggga acaggcagct
     1921 tttcgcgcaa gatcggctgc gtctttgcat aataattagc tgggacaagc ccgcttttcg
     1981 cgggctctcg aaggagattt cattgacatc ggcggtttcg acctcgcttg acagctttaa
     2041 gtgccggcgc acgctcaaag tcggcaagcg cgaatacgac tatttcgatt tgaagaaggc
     2101 ggaggccgcc ggtctcgacg gtatcggccg cctgccgttc tcgctcaaag tgctgctgga
     2161 gaatctcctg cggcacgagg acgggcgcag cgtcaccgac gacgacattc gggccgtggc
     2221 cgattggctg aaggacaagc gaagcgatcg cgagatcgcc tttcggccgg cacgcgtgct
     2281 gatgcaggac ttcacgggcg tgcccgccgt ggtcgacctc gccgcgatgc gcgacgccgt
     2341 caccaaactc ggcggcgatc cgcacaagat caatccgctg gcgccggtcg atctcgttat
     2401 cgaccactcg gtgatggtgg atgcgttcgg cacgggcgcg gcgttccagt tcaacgtcga
     2461 caaggaatat ttgcgcaata gcgagcgcta cgcgttcttg cgctggggcg cgggcgcctt
     2521 cgacaatttc cgcgtggtgc cgccgggcac gggcatctgc caccaggtca atttggagta
     2581 cttggcgcaa accgtgtgga ccaagaaaga gaacggcacg aggctcgcct tcccggacac
     2641 gctggtcggc accgacagcc acaccacgat ggtcaacggg ctttccgttt tgggctgggg
     2701 cgtcggcggt atcgaggcgg aagcggccat gctcggccag ccgatctcga tgttgatccc
     2761 cgaagttgtg ggcttccggc tcacggggac gttgcgcgag ggcgtcaccg ccaccgattt
     2821 ggtgctgacc gtcaccgaga tgctgaggcg taaaggtgtc gtcggcaagt tcgtggaatt
     2881 cttcggcacc ggtctcgaca atttgccgct ggaggaccgc gccacgatcg ccaacatggc
     2941 gccggagtac ggcgcaacgt gcggcttctt ccccgtcgac aaggagacgc ttgattatct
     3001 tgaagcgacc gcgcggaagg attcacgcgt cgaactggtc acggcatatg cgaaggcgca
     3061 agggctttac cggaaggaca agacgcccga cccggtgttc acggacacgc ttgcactcga
     3121 tcttggcgat gtggtgccgt cgatcgcggg gccgaagcgt ccgcaagatc gcgtgccgct
     3181 gacccgcgcc gcgcccgcct tcgccgaagt gctcgacaag gagtacggca aagcggcgga
     3241 agccgacaag cgcgtgcccg tcgagggtaa gaatttcgat cttggccatg gcgacgtggt
     3301 catcgccgca atcacgtcgt gcacaaacac atcgaaccca tatgtaatgg tggctgcggg
     3361 tgtgctggcg cgcaacgccg tgaaggaagg cttgagcgtc aagccttggg tgaagacctc
     3421 gctggccccg ggctcgcaag tggtgacgga ctatctggac agagccggct tgcagggcga
     3481 tctggacaag ctcggattca atctggttgg ctatggctgt accacctgca tcggcaattc
     3541 cggaccattg ccggaagaga tttccaagac catccacgac aacggtctag cggtcgcctc
     3601 tgtgctgtcg ggcaatcgca acttcgaggg gcgtgtcaat cacgacgtcc gcgccaacta
     3661 tctcgcgtcg ccgccgctcg tcgtggccta cgcgcttgct ggctcggtga cgaaggatct
     3721 tgagacccaa cctcttggcg tcggcaagaa cggccccgtc tacctcaagg acatctggcc
     3781 atcctcgaag gaggtggcga agctcgtgcg caaggctgtg aagaaatcga tgttcaagga
     3841 acgctatggc gacgtgttcc gcggcgatcc ggagtggcag gcgatcaagg tcgacgaggg
     3901 cctcacctat gactgggaca ccaagtcgac ttacgtgcag gagccgccat acttctccga
     3961 catgtcggcc gagccggcgg cgttgaccga cattgacggt gcgcggattt tggggctctt
     4021 tctcgattcg atcaccacgg accacatctc accggccggc gcgatcaaga aggacagccc
     4081 ggcgggccat tacctcacgg agaatggcgt cgccgtgcat gacttcaact cctacggctc
     4141 gcggcgcggc aatcacgagg tgatgatgcg cggtacgttc gcaaatgtcc gcatcaagaa
     4201 tcagatggtg cccgggatcg aaggcggcgt gacggtgcac tacccgtccg aagagcgcat
     4261 ggcgatctac gatgcggcca tgcgctacca ggacgagggc atgccgctcg tggtgttcgc
     4321 cggcaaggaa tacggtacgg gctcctcgcg cgactgggcg gcaaagggca cacggttgct
     4381 cggcgtgcgc gcggtcatcg cccaaagctt cgagcgcatt catcgctcga acctgatcgg
     4441 catgggcgtg ctgccgctgg tcttcgccga ggacatgtcc tgggagaagt tggggctcac
     4501 cggcagcgag accgtctcgc taaagggtct gaacgatctc aagcccgggc agtgggtcga
     4561 ggcggatatc gtttatccgg gcggcgaaac cagagccgta ccgctccagg tgcggatcga
     4621 tacgttcgac gaactggact acttccggaa cggcggaatc cttcactacg tccttcgctc
     4681 gctcgcgaag gaagccgctt aaggtcactg agttgtgacc ctccgcgagc ggattttgcc
     4741 ggaaattcag cggcgtttcc ggcattctag ggacatgagg ctagtcaccc ttcttgcagc
     4801 gcttgttggc gcggcgtgta ccgccgcgct tctggcgctc gcgttctcgg tgccgagccg
     4861 ggcggagaag cccaagctcg caacggatac gcccgtgctg atcgaactgt tcaccagcca
     4921 gggctgctcg tcctgcccgc cggcggatgc gctattgaac gagtatcgcg acaagccagg
     4981 ggtgatcacc ctgtccttct cggtcgacta ctggaactat ctcggctggc acgacacgct
     5041 gtcgagcgct gagaacagcg agcgtcagcg cgactacgcg ctcgcgcgcg gcgacggcgc
     5101 cgtctacacg ccccaagtgg tggtggacgg catcacccac gtgaacggcg ccaacgaggc
     5161 cgcgatcgag atggccatgc ggcagaccac acggcggctc agcgacgtga aggtgccgat
     5221 gtccatgcgc gcggaaggta aaagcctcgt ggtcgacatc gccgatgcgc cggagacctc
     5281 ggacatgcgc gaggccacga tctggctcgc ggtggccaag gaccaggaga ccgtcgccat
     5341 cgaccgcggt gagaatcgcg gcaaggagat cacctattcg cacccggtcc gggatttcac
     5401 gccgatcggc atgtggaagg gcgagaagat gtcgcttcgc ctgccgctga aggatctcca
     5461 gaccatcggc ggcgactgcc tcgtggccat gttgcaggta gagggctcgg gtccggtcct
     5521 gggcgctgcg gcctacgagc gcgactagcc cccaccgttt tctgcctcgc aaatacaaaa
     5581 gggccggcgt gtcgccgacc ctttcgagct gcctcttcga tgagaaatgg cccggtccga
     5641 gagaagaccc cggggggctg gggggcttag attccgaagc cctctcaagt tggaccgggc
     5701 cgaaaggcaa cggtgtttac cataaggttt tttggggcgc gcccttgact gaactggggc
     5761 gaaaccgcga attatgtttc gcattgttac agtccgtcag gggctcgcgc cgcttgaatt
     5821 tccgggtgcc agcggtcatc atgacgcttc cgttacagga ggcgcgattg agcgaaacgg
     5881 aacccatagt cgtgccgctg gccttccggg ctggcggcct acagagcccc tccgaggggg
     5941 gcgcccggcg cgacggcatc tcattcgatc gccgggaact ctcccaaatc ctcaatgtct
     6001 acgtccggaa ggtcgccagc ggcgagtggc gcgactacgc cattgatacg ctgaaggaaa
     6061 aggccgtttt ctcggttttc cggcgctcgt ccgagatgcc gctctaccgc atcgagaaga
     6121 ccccgaggct ggcgcgccgc cagggcgcct ataccgtgat cgcagccacg gggctgatcc
     6181 tcaagcgggg ccacgacctt gcccgggtct tgagggccct cgataagggc cttcgcctcg
     6241 tagaggccta attcacagcg cttaatcgga attgagagag gtccagacaa gagagaagcc
     6301 ccggagacgg ggggactcgg ggcttctccg gttccttttg gggggaacct cgtgacgggg
     6361 agggaaagac ccgccacgat acggatttgg cgatgcagcg ccgattttca acccatgcga
     6421 gccgtccgca ggccaggcca gggtaaaccg gaaaagcaaa ggccccgacg tttccgccgg
     6481 ggccttttcg catctttgga tgctttgtgc ccctagcggc ggtcgccgaa gagctgcagc
     6541 agcatcatga acaggtttat gaagtcgagg tacaggcgca aagcccccat gacggccttg
     6601 cggccagaca tagagccatc gtcgttggcg ctgtacatct ccttgatctg ctgggtgtcg
     6661 taagccgtca atccggcgaa gaccagcacg ccgatcaccg acactgcgaa ctgcagcgcg
     6721 gtcgaaccga tgaacaggtt caccagcgag gcgatgataa tgccgatcag gcccatgaac
     6781 aggaacgaac cccagcccga caggtccttg ctcgtggtgt agccccacag cgacagggcg
     6841 ccgaacgatg ccgcggtgat gaagaacacg cgcgcgatgc tttcgccggc ataaaccagg
     6901 aagatcgacg agatcgatgc ccccatcagg gccgagaaga cccagaagga tagctgggcc
     6961 gtgcccaagc tcatggagcc gatgcgcatc gataggaaga acaccatggc gagcggcgcg
     7021 aagatcacca cccacttgaa gccgctggca aacatgaagt tgccgaacgc ggtcagttcc
     7081 agcccgcccg cgccctgttg cacggccatg gcgtagattg cataggcgac gacaccggtg
     7141 atggcgactc cggacgccat gtagttgtaa acgcggatca tataggcgcg caggccctgg
     7201 tcgatggcgc cagcgtcggc gcgtccgacc gattgcccat aacgtctgtc gagttccgcc
     7261 atatacgaaa atccttctct agcgtttgtc aggaaagcca ctctgacctg aaatatggtg
     7321 gttttaggct atttctgcaa gatgaagaga gtgtgtccca tcaatcggaa atgcctatga
     7381 ggtccagtgc tcggggccta ttcggacctc agatagggcg ccgccttgac cgaaagcacc
     7441 cgccaggtgg tgaaggcgcc cacgataaga accagcgcca gcgccagtcc gatggtcgcg
     7501 gccgcggcga gcggcgcgaa cttgaactcg atgtcgaagg cgaaggtgca tagcgcccag
     7561 gcggtgatgg tggcgatgag gatcgccagc agcgacgtgg cgagcccgag cacgccgaac
     7621 tcgagcagct cggccttgac gatgagcccg cgcgtggcgc cgagcgtctt gtagagcaca
     7681 gccagatatt tccggcgctg ctggccggcg gataccgccc cagccaggac cgcggcgccg
     7741 atcagcagcg tgaagccgga cgttgcttgg attgcaccca tgagcttttc gagcatggcc
     7801 ttggccgcgt cgacgaggtc gccgatcttg atggcggtga cgaggggaaa gcgctcggcg
     7861 agcgattgaa tgatcttggc ttcctcgtcc gggtcggtgc ccttgggcag ttccagcgtg
     7921 gtgaccagtc ggtgcggtgc gccttgcagt gtgttgggtg aaaacaccat gacgaagttg
     7981 atagcaagcg actcccagtc gatcttgcgc aagctcgcga tcttggcctc cacgttgcgg
     8041 ccgaggatgt tgacggtgat ctgatcgccg agcttcagac cgagaccctt ggcgagctcg
     8101 ccgtcgaagg agaccagcgg cggtccgtca tagtccttcg gccaccattc gccttcttcg
     8161 accttcgagg cgcccggcac tgtatcggta taggtaagcc cccggtcgcc ggcgaggacc
     8221 caacgcgcgt ccggcttgac ttcgacgtcg tccacggaca ccccgttcag cttgacgatg
     8281 cggccgcgca acatgggcgc gttgtcggtc tttgccttcg gctggatctc cttggtcgtc
     8341 tgctcgaagg tcgccaaatc gccgggctcg acatcgagga agtaataggc gggcgcgtcg
     8401 acctccacat ggctctcaat ctcggtgatc agcgagtggt ggatcagggc cacggcgatg
     8461 agaaggccga gtcccagccc caacgacacg gcgatggatc gggcgagcga gcctggcgcg
     8521 ccgatactgg cgagcgcgag cccccaggcc ggcggcttgg tgcggcgata gcgtgccgcg
     8581 aggcgctgca cgagaagtcc aaagccaagc agcagtccga aggcggcgag gatccccagt
     8641 gaaatcgccg cggtgacaag acgttcttcg gaagcggcaa tggcgagcac gaatagggcc
     8701 agccccgccg cggccgagcc gagcgcgaac gcggtcgtcg accgctcgcg ctcttcggtg
     8761 agatgggcgc gcatcaacac ggcgggcgaa atgcggctgg cgcggccgag cggccacagc
     8821 acgaacagca ccatggtcag gagcccggcg agcgccgcca cgatgagcgg catgggatgc
     8881 ggctcgaccg caagcctgag gggaagcgtg tcctcgtaaa
//
LOCUS       DBNHVU010000131         4635 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1090812, whole genome shotgun sequence.
ACCESSION   DBNHVU010000131 DBNHVU010000000
VERSION     DBNHVU010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1090812"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="AC16Y7_05225"
     CDS             <1..254
                     /locus_tag="AC16Y7_05225"
                     /inference="COORDINATES: protein motif:HMM:NF019229.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ASPIC/UnbV domain-containing protein"
                     /protein_id="HIR5708813.1"
                     /translation="RFLSVRLPDTPQSIGARVRLEGIHAPVYTYIAGEGLSSDRTTQF
                     VIGVPKNSPAPTALIVEWFDGAVSQIADPPMNKTIVVAP"
     gene            complement(727..1170)
                     /locus_tag="AC16Y7_05230"
     CDS             complement(727..1170)
                     /locus_tag="AC16Y7_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708814.1"
                     /translation="MIPDEPLGEEGGAHGVDWDAVRHDFLYSGMSQRRIAWKHGATAG
                     TLRKYMRDGGWERQIRQVNTPRCHARVCPLFKRREGLMAIQLTPPTKNVFYLSIICAV
                     VAIVLYILGVLGVIGGGFMAVAHIAFWIGMLGWALLMAGVAMKGV"
     gene            1319..1483
                     /locus_tag="AC16Y7_05235"
     CDS             1319..1483
                     /locus_tag="AC16Y7_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708815.1"
                     /translation="MFRTIAIWVFGLIASALAGMTIGAVLMEQGAGLGLIAGLAIFTI
                     ARLLLTMPRS"
     gene            complement(1652..1867)
                     /locus_tag="AC16Y7_05240"
     CDS             complement(1652..1867)
                     /locus_tag="AC16Y7_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708816.1"
                     /translation="MAELCVEVLENGLRVTQRGTPFVVTYMRNQGSQLLEAQELLALP
                     EIDGQQASFLAAAWKAAYAKARDLGWL"
     gene            2525..2821
                     /locus_tag="AC16Y7_05245"
     CDS             2525..2821
                     /locus_tag="AC16Y7_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708817.1"
                     /translation="MRIIYLAFVMFGLVLVLGLGVAQSDAPAPQQVADAAMGLSLPGA
                     FTQLPKTKIMSPVRADVAPLPFCGQCSQDSDCGTGHKCCGPSSCLECFRVVTCP"
     gene            complement(2841..3230)
                     /locus_tag="AC16Y7_05250"
     CDS             complement(2841..3230)
                     /locus_tag="AC16Y7_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708818.1"
                     /translation="MPNFAFTHRVVALRLVLTAAVLALGVPTLAQDGRAQDAFDLSVP
                     KPGEEAAAPANLDCGTEMLSGSGPGFTADRDDAEEAAINGWLEKAHTIYPEATWDMAK
                     DSNLSCAVQGLYSKCFADAIPCKPKPE"
     gene            3621..3914
                     /locus_tag="AC16Y7_05255"
     CDS             3621..3914
                     /locus_tag="AC16Y7_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708819.1"
                     /translation="MTRFCVALFAAGLLASGLGACSRKSAAPDVSFLPTRAVAQATED
                     SKGAFEAAKEVCLEEARRKGISSVTRILLFRGKTSKSDYVDCMERKGFAVVDD"
     gene            4142..>4635
                     /locus_tag="AC16Y7_05260"
     CDS             4142..>4635
                     /locus_tag="AC16Y7_05260"
                     /inference="COORDINATES: protein motif:HMM:NF013106.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR5708820.1"
                     /translation="MRDFLQGNGRGKFIDWLKIDSWIDSGLYGAWIGFRDWWSAYSSF
                     FGRFEVKGFWRACNELACEALTLSVAGFLVVLTFALPAFEIAQGKINLSDEFSVTFLD
                     RYGNEIGKRGLLRDDSVPLEEIPDHMIKATLATEDRRFFDHFGIDIMGTLRALAENAQ
                     ADRVV"
BASE COUNT          815 a         1491 c         1451 g          878 t
ORIGIN      
        1 gccggtttct gtccgtccga ctcccggata cgccgcaaag cattggcgcg cgcgtccgtc
       61 ttgaaggcat ccacgctccc gtgtacacct acatcgccgg cgaggggctg tcctcggacc
      121 gtacgacgca attcgtcatc ggggtgccga agaacagccc ggcgcctacg gcgcttatcg
      181 tcgagtggtt tgacggagcg gtttcgcaga tcgccgatcc gccgatgaac aaaacaattg
      241 tggtcgctcc gtaagcgcat gagacgtcgt cgccgaggtt gacgtcgacc ttggcgaaga
      301 gggccgtggc ttgcgtcacg ttgaaaaact tgacgctggc cgagatctcg ccccatacgt
      361 cttcgagatc gtcgttgaag cggaacgtgg tgcgggtgga taccaaggtg gcgctgtcga
      421 gcaaagcgag cttcgcgccc cgctcggcga aggcgttcgt tagtgtacga ccgaagcctt
      481 gcgccgcccc ggtaatcaaa acgaccttgt ccttgaaatt gaaaactttg cctcagagga
      541 cgcaattgta acgcgctttt tggacttagc aggcgcgcat cgcccgggtg gcgcaacgat
      601 acagaccact tcacagacgg gcgcgcacct gacctcacac cccggcagca cctcgccaaa
      661 gcgttgcagc attatgaaag tctcttcagg ttcggggcgg aggcgccccg gggagacctg
      721 ccggccctag acgcccttca tggcgacgcc tgccatcagc agcgcccagc ccagcatacc
      781 gatccagaag gcgatatggg ccacggccat gaagccgccg ccgatgacgc cgaggacgcc
      841 aaggatatag agcacgatcg ccacgaccgc gcagatgatg gacagataga agacgttctt
      901 cgtcggtggt gtcagctgaa tggccatgag tccctcccgt cttttgaaaa gcgggcagac
      961 cctagcatga cagcgcggcg tgttcacctg ccggatttgc cgttcccagc cgccgtcgcg
     1021 catgtatttg cgtaaagttc cggccgtggc gccgtgcttc caggcgatgc gccgctgcga
     1081 catgccgcta tagagaaagt cgtggcggac cgcgtcccag tcgacgccgt gcgcaccgcc
     1141 ctcctcccca agaggttcgt ccgggatcat ggcgtactgt tcttgttgtt gtatgtgcgc
     1201 cctccctccc cctgccggag cgagggaaaa tccgccgtgc ttagagtttg ccgctccgga
     1261 aacgaacgtc tatcgaagtg cctgcatatc aagccgctgc cgcgcgcctg gaggcgacat
     1321 gtttcggacc attgcgattt gggtcttcgg tctcatcgcc agcgcgcttg ccgggatgac
     1381 gatcggcgcc gttctgatgg agcaaggcgc aggcctcggg ctgatcgccg gcctcgccat
     1441 tttcaccatc gcgcgtcttt tgctgaccat gccccggtcg tagacgcgag gacccggcgc
     1501 tagaaactaa caacgccgcc ccattgctgg gcgcggcgtc tcaacgcggt ggatcgaaac
     1561 ggtgtctcgc ccacgtgtcg cgttgtagcc gaacactagg caaacagcgt cacgctgtca
     1621 aggacttttt tcctaggctt gtcgagcgcc ctcaaagcca gccgagatcc ctagccttgg
     1681 cgtaggcggc cttccacgcc gcggcgagga aggaggcttg ttgtccgtcg atttccggca
     1741 gcgccaggag ctcttgagct tccaggagct gcgacccctg gttcctcata taggtcacga
     1801 cgaacggcgt gccgcgctgg gtcacgcgaa gaccgttctc caagacttcg acgcagagct
     1861 cggccatcgc gaacccgttt cctcggcact ccccggagcc aagattttag gcgtgtacgg
     1921 attggtgaca acccgttttg tgacaccagc taaagagaaa gccccggctc gctttcgcaa
     1981 gacgccgggg ctttcgctag ggcactttat caggtgaggc cacgctagcg gccgtatgtg
     2041 acaggctcgt tacgccggcc cgcgcgcgat tgccgcgggg gtcgacgccc catccccgat
     2101 tgaaccacga ggtaagactt catttaacca agccggggtc agcatgggca ggatatcaac
     2161 gatgggagag ccccatgcat cccacccgta cccgccggaa tcggcggcgc actgcccgct
     2221 cctggcgcaa gagccgcgag cccgcccggg cgccgctcca cctcgcggcg gcagaaccgc
     2281 ttctcgccgc tggcggcctg agcccgagct atcgccgctg cacaagatcc cgacgctcag
     2341 gccttggctc gcgacccgcg acaccgccat ggagcacggc gcgcacgccg ccggctgacg
     2401 gcgccctcgt ccgccaagcc cttcggagcg gggcccttct ctcaaggccg gcgcaggcgt
     2461 aagatcgacg cccggcgcgg gagcgtctcg tgtccccgcg cggaacacga gagaggatgc
     2521 tgccatgcgg attatctatc tcgctttcgt catgttcggc ttggtgctcg ttctcgggct
     2581 tggcgtcgcg cagagcgatg cgccggcccc acagcaggtc gccgacgcgg ccatggggct
     2641 aagccttccg ggcgccttca cgcaattgcc caagaccaag atcatgtcgc ccgtccgggc
     2701 cgacgtcgcg ccgctgccat tctgcggcca gtgctcgcag gactccgatt gcggcacggg
     2761 ccacaaatgc tgcgggccct cgagctgcct ggagtgcttt agggtcgtga cctgtcctta
     2821 aaaagaagcc tgccgcggtg ctattccggc ttcggcttac agggaatcgc gtccgcgaag
     2881 cacttcgagt agagcccctg cacggcgcag ctgagatttg agtccttcgc catatcccat
     2941 gtggcttcgg gatagatggt gtgggccttt tccagccagc cgttgatcgc cgcttcctcg
     3001 gcgtcgtcgc ggtccgccgt gaagcccgga ccgctgccgg acagcatttc ggtgccgcag
     3061 tccagattgg ccggtgcggc cgcctcttcg cccggcttcg gcacggacag gtcaaacgcg
     3121 tcttgcgccc ggccatcctg ggcaagcgtc gggactccaa gcgccagaac agcagccgtc
     3181 agaacaaggc gcaaggcaac gacgcggtgg gtgaaggcga aattcggcat cgcgtaaagg
     3241 ctccctccca aatgttccct ttcaagggtg ttctctttgt cggcccctcg tttgcggggg
     3301 agtcttagca cagactatcc gtgaggctga atacgctgcc gcgccggaaa tgctggcaga
     3361 aggcttgccg gctgagcagg ccctgctccc tgtccatgat gctgccttgg tttgcgccga
     3421 cactccgccc gatgcgcccc tccgccgcgc cagggaaccg gcgagggtgc gcgttccttc
     3481 cggccttaac ctttgaccgg cctctcggta caagggccca ccggaaggga ctaggttgaa
     3541 agaggacggc cgggagcggc ggggccttca gggtcgcgcc gttagattcg tggccgaagg
     3601 tctgtcgggg gattgtcatc gtgacgaggt tctgcgtggc gctatttgcg gccgggctct
     3661 tggcctcagg ccttggcgcg tgcagccgca agtccgctgc gcccgacgtt tccttcctgc
     3721 cgacccgggc cgtggcccaa gccaccgagg actccaaagg cgccttcgag gccgccaagg
     3781 aggtgtgcct ggaggaggcc cgccgcaagg gtatctcctc ggtgacccgg atcctgctgt
     3841 tccgcggcaa gacctccaaa tccgactatg tggattgcat ggagaggaaa ggcttcgcgg
     3901 tcgtggacga ctagcgccgc cgccggtccg gtgcgtggtt agcggcgtct taacgcagac
     3961 ggggacaagc tgggccggct tcgggcgctg agacgccgga ccttcgattt cgggctcgcg
     4021 gaacaccgcg gattctctat aaatagcccc aatcagggta tactgggctt tagggtcgcc
     4081 cggacggacg gccgggtata gcgcacccga taagggctgc gagcaaagag gtcgggttga
     4141 attgcgggac ttcttacaag gaaatggccg cggcaaattc attgattggc tgaagatcga
     4201 ttcatggatc gattccggcc tctatggcgc ctggatcggc ttccgcgact ggtggagcgc
     4261 ctattcgtcc ttcttcggcc gtttcgaggt caagggattc tggcgcgcct gcaatgagtt
     4321 ggcttgcgaa gcactgacgc tgagcgtggc cggattcctg gtcgtgctca catttgccct
     4381 gcccgccttc gaaatcgcgc aaggcaagat caacctctcc gacgaattca gcgtcacctt
     4441 cctggatcgc tatggcaacg agatcggcaa gcgcggcctg ttgcgcgatg actccgtgcc
     4501 gctggaagaa attcccgatc acatgatcaa ggcgacgctc gccaccgagg accgccgctt
     4561 cttcgatcac ttcggcatcg acatcatggg tacgctccgc gccctcgccg agaacgccca
     4621 agcagatagg gttgt
//
LOCUS       DBNHVU010000132         7546 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_793663, whole genome shotgun sequence.
ACCESSION   DBNHVU010000132 DBNHVU010000000
VERSION     DBNHVU010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7546)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_793663"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1190
                     /gene="nusA"
                     /locus_tag="AC16Y7_05265"
     CDS             <1..1190
                     /gene="nusA"
                     /locus_tag="AC16Y7_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR5708821.1"
                     /translation="FRYGDRIRAFVYDVRREQRGPQIFLSRTHPEFMAKLFAQEVPEV
                     YEGIVTIKSVARDPGSRAKIAVVSRDASIDPVGACVGMRGSRVQAVVNELQGEKIDII
                     QWSPDAATFIVNALAPAEVVKVVLDEDAERIEVVVPDEQLSLAIGRKGQNVRLASQLT
                     GWDIDIMTEASESERRQAEFAERSAKFMEALDVDEVIAQLLASEGFSSVEEVAFVEPT
                     EISSIEGFDEETAAEIQTRAREHLEKLEAELNAKRKELGVADELMEVPGLNTAMLVAL
                     GENDVKTVEDLADCATDDLAGWNERQEKETTHYDGFLEASGLTRTQCEDLILAARVKA
                     GWIDEADLVADEEEAEGEEVDGEVEGESGAEAAETTSEGQTETETKTETGEKPAEDDA
                     SAS"
     gene            1212..1892
                     /locus_tag="AC16Y7_05270"
     CDS             1212..1892
                     /locus_tag="AC16Y7_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HIR5708822.1"
                     /translation="MRIEATEIGETKGQPATDTLRRCAVTRVQRPKDDLIRFVLGPDG
                     TVVPDLKEKLPGRGVWLTAAHETVAQAVKRKVFSRALKAEAKASEGLADQVGALLTET
                     ALQALGIANKAGNVVFGHTKVEQALGAGSGKGHVVALIHASDAADDGCRKLDGKARAT
                     TEGRGFPTVRAFSADELSLASGRPNVIHAALIPGGAARKFLAAASRVERYRMGPSAFA
                     DRYGLDTD"
     gene            1894..4389
                     /gene="infB"
                     /locus_tag="AC16Y7_05275"
     CDS             1894..4389
                     /gene="infB"
                     /locus_tag="AC16Y7_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011583238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR5708823.1"
                     /translation="MSEANESDGKTSGRKMTLNVRRTVESGHVRQSFSHGRSKSVVVE
                     KKKRRTIGSGSAAPTVEEPKTKPKAKAAEPAPKPAPEEKQRRSGVVLKQLSEEEKGAR
                     AKALADAKVRQAEEKERAKENATQEAQLAEQQATEAREAEERKRKEEEEKAKAAAEEK
                     AKPKTVELQRSKHLKPEAEKQPSRAPDARHKKGPAAPRRGEERRVRGRLTITNALDEE
                     QRQRSLASLKRHRERQKKQGAGPQSTQKIAREVTIPEAITIQELANRMAERGVELIKH
                     LMKDGEMHKITDVIDADTAELIAEEFGHTVRRVSEADVEEGFIGDDDDAENLQPRAPI
                     VTIMGHVDHGKTSLLDSLRAANVVASEAGGITQHIGAYQVTVPSGQKITFIDTPGHEA
                     FTAMRARGAKVTDIVILVVAADDGVKPQTIEAINHAKAAEVPLIVAINKIDTPGADPN
                     KVRTELLSHEIVVESMGGDTLELEVSALKKTNLDKLLEAIVLQAEVLELTANPDRPAE
                     GVIVEAKLERGRGPVGTALIQRGSLKLGDIVVAGSAWGRVRALINDVGDQVKEAGPSV
                     PAEVLGFDSAPEAGDQFAVVENEARAREITDYRDRERRKRQAVAGQRGSLEQMMTQLQ
                     ETGVQEFPLVIKGDVQGSVEAISAALANLGTDEVRARVVHSGVGGITESDVSLASTSG
                     AVLLAFNVRANTQAREHAARDGVEIRQYAVIYDLVDDIKAAMSGLLAPTVRETFLGNA
                     EILEVFNISKVGKVAGCRVSEGKVERGAHVRLIRDNVVVHEGKLSTLKRFKDEVKEVV
                     AGQECGMAFEGYQDMRQGDVIECFEVEHVQRTL"
     gene            4439..4828
                     /gene="rbfA"
                     /locus_tag="AC16Y7_05280"
     CDS             4439..4828
                     /gene="rbfA"
                     /locus_tag="AC16Y7_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015951187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR5708824.1"
                     /translation="MTPRDKGRGPSQRQLKVGELIRHALAEVLSRGDVMDDVIARHSL
                     TVPEVRMSPDLKLATCFVMPLGGGEADDVVAHLEKHKRFLRGELASRVSLKFMPELRF
                     KVDTSFKTSARIDELLASPQVARDLDE"
     gene            4852..5772
                     /gene="truB"
                     /locus_tag="AC16Y7_05285"
     CDS             4852..5772
                     /gene="truB"
                     /locus_tag="AC16Y7_05285"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR5708825.1"
                     /translation="MARRRKGLAISGWLILDKPEGMTSTKAVGRVRWLYNAAKAGHAG
                     TLDPLATGILPIALGEATKTVPFVVDGSKTYRFTVRFGAETDTDDTQGKVLETSDRRP
                     TLSEIEAALPRFTGEISQIPPRYSAIKVEGARAYDLARDDEDFVLEPRIVNVDRLDVV
                     DQPDADHCVLEADCGKGTYVRALARDLGRALQCLGHVSALRRTKVGAFTEDRAVPLSV
                     LEECADESRETLMARLLPVEVGLEDLPSLTMSSADASQLRQGRAVLLRGRDAPLVTGP
                     VYAVSKGELVALGEVEEGEFRPRRIFNLSS"
     gene            5930..6199
                     /gene="rpsO"
                     /locus_tag="AC16Y7_05290"
     CDS             5930..6199
                     /gene="rpsO"
                     /locus_tag="AC16Y7_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR5708826.1"
                     /translation="MSISAERKQELIKEYATKTDDTGSPEVQVAILTERISNLTGHFK
                     THGKDNHSRRGLLKLVSQRRRLLDYVKSTNEDRYQKLINRLGIRR"
     gene            6303..>7546
                     /gene="pnp"
                     /locus_tag="AC16Y7_05295"
     CDS             6303..>7546
                     /gene="pnp"
                     /locus_tag="AC16Y7_05295"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR5708827.1"
                     /translation="MFDKHTVEIDWGRRPLKLETGRVARQADGAVLATYGDTSVLATV
                     VSAKEARVGIDFFPLTVNYQEKTFAAGKIPGGYFKREGRPTEKETLTSRLIDRPIRPL
                     FAKGFKHETQVVTTVLSHDMENDPDIVALVAASAALTLSGVPFLGPIGAARVGYIDGK
                     YVLNPMVDEMPETQLDLVVAGTTDAVLMVESEAKELPEDIMLGAVMYGHEHFQPVLDA
                     IVKLAERAAKEPWELKTVDNSEMVEKVRGMADSGLREAYAIASKQERRDGISQIKEKI
                     VTELVPDDDDGTLAGKVSGAFKEAEKDVVRGSILDTKKRIDGRDLETVRPISCEVGIL
                     PRTHGSALFTRGETQALVVTTLGTGEDEQFVDALQGTYKETFLLHYNFPPYSVGETGR
                     MGSPGRREIGHGKLAWRAVHPI"
BASE COUNT         1518 a         2372 c         2500 g         1156 t
ORIGIN      
        1 atttccgcta tggcgaccgc atccgcgctt ttgtctacga cgtgcggcgc gagcagcgcg
       61 gcccgcagat cttcctgtcg cgcacgcatc ccgaattcat ggcgaagctg ttcgcgcagg
      121 aagttccgga agtctacgaa ggcatcgtca ccatcaagtc ggtggcgcgc gatccgggca
      181 gccgcgccaa gatcgcggtc gtctcccgcg acgcctccat cgatccggtt ggcgcgtgcg
      241 tgggcatgcg cggcagccgc gtgcaggcgg tggtgaacga gcttcagggc gagaagatcg
      301 acatcatcca gtggtcgccc gacgccgcca cgttcatcgt gaacgcgctt gctcctgcgg
      361 aagtggtcaa ggtcgtgctc gacgaagacg ccgagcgcat cgaggtggtc gtgccggacg
      421 agcagttgtc gcttgccatc ggccgcaaag gccagaacgt gcggctcgcc tctcagctca
      481 ctggctggga catcgacatc atgaccgagg cttcggagtc ggagcggcgc caggcggagt
      541 tcgccgagcg ttcggccaag tttatggaag cgctcgacgt ggacgaggtg atcgcccagt
      601 tgctcgcttc ggaaggcttc tcctcggtgg aggaagtggc cttcgtcgag ccgacggaga
      661 tctcctccat cgaaggcttc gacgaggaaa cggccgccga gattcagacc cgggcgcgcg
      721 agcaccttga gaagctcgag gcggagctga acgccaaacg taaggaactc ggcgtcgccg
      781 acgagttgat ggaggtgccc ggtctcaata ctgcgatgct ggtggcgctt ggtgagaacg
      841 acgtaaagac cgtcgaggac ctcgccgact gtgccaccga cgacctggcc ggctggaacg
      901 agcgccagga aaaagagacc acgcactacg acggctttct tgaggcctcc ggcctgacgc
      961 ggacgcaatg cgaggacctg atcctggcgg cgcgggtgaa ggccggctgg atcgacgagg
     1021 ccgatctcgt ggccgacgag gaagaggcgg aaggcgaaga ggtggacggc gaagtcgagg
     1081 gcgaatccgg ggccgaggcg gccgagacca cttccgaagg ccagaccgag actgagacca
     1141 aaactgaaac gggcgagaag cctgctgaag acgacgcctc cgcgtcatga ggcggttagg
     1201 agacagagct gttgcggatc gaggcgacgg agataggcga gaccaaaggg cagccggcaa
     1261 cggatacgtt gcgccgctgc gcggtcacgc gcgtccagcg tcctaaggac gacctcatcc
     1321 gcttcgtgct cgggcctgac ggcaccgtcg ttcccgacct gaaggaaaag ctccccggcc
     1381 gcggcgtctg gctgaccgca gcccatgaga ccgtggcgca agcggtcaag cgcaaggtct
     1441 ttagccgggc actgaaagcc gaggcgaagg cctcagaagg ccttgccgac caggtcggcg
     1501 cccttctcac cgagaccgcg ctccaggcac tcgggatcgc caacaaggcg ggcaacgtgg
     1561 ttttcggcca caccaaggtc gaacaggcgt tgggcgcggg aagcggcaag gggcacgttg
     1621 ttgcgctgat tcacgcctcc gacgccgccg acgatggctg ccggaaactt gatggaaaag
     1681 cccgcgcaac caccgaagga cgcggtttcc caacggttcg cgccttcagc gctgatgaat
     1741 tgagtttggc atcgggcagg ccaaatgtga tacatgctgc cctaatcccg ggcggagcgg
     1801 ctcgaaaatt tctcgcggcg gcctcgcgcg ttgagcgcta ccgcatgggt ccctccgcat
     1861 tcgcggacag atacggattg gatacggact agaatgagcg aagcgaacga gagcgacggc
     1921 aagacgagcg ggcgcaagat gacgctcaat gtgcgccgaa ccgtggagtc tggccatgtg
     1981 cgccagagct ttagccatgg gcgctcaaag tccgttgtgg tcgagaagaa aaagcgccgc
     2041 acgatcggct cgggcagcgc cgcaccgact gttgaagagc ccaaaaccaa accgaaggca
     2101 aaggccgccg aaccggcccc caagccggcg ccggaagaga aacagcgccg ctcgggcgtg
     2161 gtgctgaagc agctttccga agaggaaaag ggtgcccgcg ccaaggcact cgccgatgcg
     2221 aaagtccggc aggctgagga aaaggagcgc gctaaggaga acgcgaccca ggaggcccag
     2281 ctggccgagc agcaggccac ggaggcgcgc gaggccgagg aacgcaagcg gaaagaagaa
     2341 gaagaaaagg caaaggccgc cgccgaggaa aaagccaagc cgaagaccgt ggagctgcag
     2401 cgcagcaagc atctcaagcc ggaagcagag aagcagcctt cgcgcgcccc cgatgcccgc
     2461 cacaagaagg gccccgctgc cccccggcgc ggcgaggagc ggagggtccg cggccgcctt
     2521 accatcacca acgcgctcga cgaggagcag cgtcagcgga gccttgcctc gctcaagcgt
     2581 caccgcgagc gccagaagaa acagggtgcc ggaccgcaat cgacgcagaa gatcgcgcgt
     2641 gaggtcacga tccccgaggc catcaccatc caagagctcg ccaaccgtat ggccgagcgc
     2701 ggcgtcgaac tcatcaagca cctgatgaag gacggcgaga tgcacaagat caccgatgtg
     2761 atcgacgccg atacggcgga gctcatcgcc gaggagttcg gtcataccgt ccggcgcgtg
     2821 tccgaagccg atgttgaaga aggcttcatc ggtgacgacg acgatgccga gaatcttcag
     2881 ccgcgcgcgc cgatcgtcac gatcatgggc cacgtcgatc acggcaagac ctcgctgctc
     2941 gactccttgc gcgccgcgaa cgtcgtcgcc tcggaagccg gcggcatcac ccagcatatc
     3001 ggcgcctatc aggtcaccgt gccgtcgggc cagaagatca ccttcatcga cacgcccggc
     3061 catgaagcgt tcaccgccat gcgcgcccgc ggcgcgaagg tgacggacat cgtgatcctg
     3121 gttgtggccg ccgacgacgg cgtgaagccg cagacaatcg aggcgatcaa ccacgccaag
     3181 gccgccgaag tgccgctgat cgtggccatc aacaagatcg acaccccggg ggccgaccca
     3241 aacaaagtcc gcaccgagct gcttagccac gagatcgtgg tggagagcat gggcggagac
     3301 acgcttgagc ttgaggtctc ggcgctcaaa aagacgaatc tcgacaagct gcttgaggcc
     3361 atcgtgctcc aagctgaagt gctcgaactc accgccaatc ccgatcggcc cgccgaaggc
     3421 gtcatcgtcg aggcgaagct ggaacgcgga cgcgggcccg tgggcaccgc gctgatccag
     3481 cgtggctcac tgaagctcgg cgacattgtg gtggcaggct ccgcctgggg ccgcgtgcgc
     3541 gcgctgatca acgatgtcgg cgaccaagtc aaggaagccg gaccgtcggt cccggcggaa
     3601 gttctcggtt tcgattccgc gccggaagcg ggcgatcagt tcgccgtcgt ggagaacgag
     3661 gcgcgggcgc gcgagatcac cgactaccgc gatcgcgagc ggcgcaagcg gcaggccgtt
     3721 gccggccagc gcggcagcct cgagcagatg atgacccaac tccaggagac cggagtccag
     3781 gagtttccgc ttgtcatcaa gggcgacgtg cagggttcgg tcgaggccat ctcggcggcg
     3841 ctcgccaatc tgggcaccga cgaagttcgg gctcgcgtcg tgcattcggg cgtgggcggc
     3901 attacggagt cggacgtgtc tctggcatcg acgtcaggcg cggtactcct cgccttcaat
     3961 gtgcgcgcca acacgcaggc ccgcgagcac gctgcccggg acggcgtcga aatccgtcag
     4021 tacgcggtga tttacgacct ggtcgacgac atcaaggcgg ccatgtccgg cctgcttgcc
     4081 ccgaccgtgc gcgagacatt cctcggcaac gccgaaatcc tggaggtgtt caacatctcc
     4141 aaggtcggca aggtggccgg ctgccgcgta agcgaaggaa aggtcgagcg tggcgcccat
     4201 gtccggctga tccgcgacaa cgttgtggtg cacgaaggca agctgtccac gctcaaacgc
     4261 ttcaaggacg aagtgaaaga ggtggtggcg ggccaggaat gcggcatggc gttcgagggc
     4321 taccaggaca tgcgccaagg cgacgtgatc gagtgtttcg aggtcgagca cgttcagcgc
     4381 acgctgtagg cctgagcgcc cgcgtcatcc attcggcgct ttcgagagaa gccagacaat
     4441 gaccccgcga gacaagggcc gcggcccgag ccaacgccaa ttgaaagtcg gagagctgat
     4501 ccgccacgca ctggcggagg tcctttcgcg cggcgacgtc atggacgacg tgatcgcgcg
     4561 tcattcgctc acggttcccg aagtccgtat gtcgccggat ctcaagctcg cgacctgctt
     4621 cgtgatgccg ctgggcggcg gggaagcgga cgatgtcgtc gcacacctgg agaagcacaa
     4681 gcgcttcttg cgcggtgagc tggccagccg cgtctcgctc aaattcatgc cggagctgcg
     4741 cttcaaggtc gatacctcgt tcaagacgtc ggcgcggatc gatgaactgc tcgcctcgcc
     4801 gcaagtcgcg cgcgatctcg acgagtaggc gattcgcaag ggagcgacgg catggcgcgg
     4861 cggcgcaagg gcctggcgat cagcggttgg ctgatcctcg acaagccgga aggcatgact
     4921 tcgaccaagg ccgtgggccg cgtccggtgg ctctacaacg cggccaaggc gggccatgcc
     4981 ggcacgctcg acccacttgc cacgggcatc ctgccgatcg cgcttggcga ggcgaccaag
     5041 accgtgccgt tcgtggtgga cggctccaag acctaccgct tcacggtgcg ctttggcgcc
     5101 gagaccgaca ccgacgacac gcaaggcaag gtgctggaga cgtcggaccg gcggccgacg
     5161 ctctcggaga tcgaggcggc gctgccacgc ttcaccggcg agatttcgca aatcccgccc
     5221 cgctactcgg cgatcaaggt cgagggcgcg cgggcctacg atctggcccg tgacgacgag
     5281 gacttcgtac tggagccgcg gatcgtgaac gtcgaccggc tcgatgtggt ggaccagccg
     5341 gacgccgatc attgcgtgct ggaggcggat tgcggcaagg gcacctatgt gcgcgcgctg
     5401 gcacgcgatc tcggccgggc gcttcagtgc ctcggccatg tgtcggcact tcggcgcacg
     5461 aaggtgggcg ccttcacgga ggatcgcgcc gttccccttt cggtattgga ggaatgcgcc
     5521 gatgagagcc gggagacgct gatggcaagg ctgctaccgg tggaggtggg gcttgaagac
     5581 cttccgtcgc tgaccatgag ttcggccgac gccagccagc tccgccaggg gcgcgccgtt
     5641 ctcctcaggg gccgggacgc gccgctcgtc actggcccgg tctacgcggt ttccaagggc
     5701 gaattggtcg cacttgggga ggtcgaggaa ggcgaattca ggccccggcg gatctttaac
     5761 ctttcgtcct aggcgcccaa attccgctgg tctctagccc cataatccct atagtgcagc
     5821 gcatcgtgcg ggggccggat ttggcccctg tcgcgattct agaaccgacc gtgctggacg
     5881 acatcccggc tcggccctgt caaaaccctc aaattacaag gatatcccga tgtcgattag
     5941 cgctgagcgc aagcaagagc ttatcaagga atacgcgacc aagacggacg ataccgggtc
     6001 gccagaagtt caggtcgcga tcctcacgga gcggatcagc aatctgaccg gccattttaa
     6061 gacacatggc aaggataacc attcgcggcg cgggctcctg aaactcgtga gccagcggcg
     6121 ccggctgctc gactacgtca aatcgacgaa cgaggacagg taccagaagc tcatcaaccg
     6181 gctcggtatc cgccggtagc tttccgttga aaatgcggcc cgagcttcca tgcatataac
     6241 cgggctcccg cgccaccggg cgcgggatag ccttatcttt ccccacttac tgggacacac
     6301 acatgttcga taaacacacc gtagaaattg attggggccg acgcccgctg aaacttgaga
     6361 ccgggcgcgt cgcccgccag gccgacggcg cagtgctcgc cacctatggc gatacatccg
     6421 ttctcgccac cgtcgtctcg gccaaagagg caagagtcgg gatcgacttc tttcccctca
     6481 ccgtgaatta ccaagagaag accttcgccg cgggcaagat cccgggcggc tacttcaagc
     6541 gtgaaggacg cccgaccgag aaggagacgc tcacgtctcg cctgatcgac cggccgatcc
     6601 gtccgctgtt cgccaagggc ttcaagcacg agacgcaagt cgtcaccacc gtgctgtcgc
     6661 acgacatgga aaacgacccg gacatcgtgg ccttggtggc tgcgtccgcc gcgctcacgc
     6721 tctctggcgt gccgttcctc ggacccatcg gcgccgcgcg cgtcggctat atcgacggca
     6781 agtatgtgct gaacccgatg gtggacgaga tgcccgagac gcagctcgac ctcgtcgtcg
     6841 ccggcaccac cgacgccgtg ctcatggttg agtcggaggc caaggagctt cctgaggaca
     6901 tcatgctcgg cgccgtgatg tacgggcacg agcacttcca gcccgtcctc gacgccatcg
     6961 tcaagctcgc cgaacgcgcg gccaaggagc cgtgggagct caagaccgta gacaactcgg
     7021 agatggtcga gaaggtccgc gggatggccg atagcggtct gcgcgaagcc tacgccatcg
     7081 cctccaagca ggagcgccgc gacgggatct cccagatcaa ggagaagatc gtcaccgagc
     7141 tcgtgccgga cgacgacgac ggcaccctcg ctggcaaggt ctccggcgcc ttcaaggaag
     7201 ccgagaagga cgtggtccgc ggcagcatcc ttgacaccaa gaagcgcatc gacggacgcg
     7261 acctagagac ggtgcgtcct atttcatgtg aagtcggcat cctgccgcgc acgcatggca
     7321 gcgcgctgtt cacccgcggc gagacgcaag cgctggtggt cacgaccttg ggcaccggtg
     7381 aggacgagca gttcgttgac gcccttcagg gtacgtacaa agagacgttc ctgctgcact
     7441 acaacttccc gccctactcg gtcggtgaga ctgggcgcat gggctctccg gggcggcgcg
     7501 agatcggcca tggcaagctc gcttggcgtg ccgtccaccc gatttt
//
LOCUS       DBNHVU010000133         2692 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_719141, whole genome shotgun sequence.
ACCESSION   DBNHVU010000133 DBNHVU010000000
VERSION     DBNHVU010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_719141"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1405
                     /locus_tag="AC16Y7_05300"
     CDS             173..1405
                     /locus_tag="AC16Y7_05300"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HIR5708828.1"
                     /translation="MHNDFHRIKRLPPYVFEKVNRLKAEARARGDDIIDFGMGNPDMP
                     TPPHIVDKLVETLRDPRTSRYSASKGIGGLRRAQAAYYDRRFGVKLDPDTQVVVTLGS
                     KEGFANMAQAITAPGDVILVPNPTYPIHEFGFIMSGGVIRHLPASTDSQFMVSLDKAV
                     RHSVPKPSALVLNYPANPTAVVANRNFYRDVVAYAKRNDLIVLSDLAYAEIYFDDEPP
                     PSILEIPGAMDIAVEFTSVSKTYSMPGWRIGFAVGNERLIQALTRVKSYLDYGAFTPI
                     QVAATAALNGEQGCVDEVRGVYRRRRDCLVDALARAGWPIPSPPATMFAWAPIPDMFA
                     HVGSLEFSKLLLEKASVAVSPGVGFGEYGEGYVRIALVENEQRIRQAARNIRKFLTES
                     ANSLHNVIPIASKAQRTF"
     gene            1415..>2692
                     /locus_tag="AC16Y7_05305"
     CDS             1415..>2692
                     /locus_tag="AC16Y7_05305"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR5708829.1"
                     /translation="MADRLKLGLAGIGTVGGGLLRLLEAREAVLDARASRPIEVVAVS
                     ARSRRKKRSVDLSGVRFVADPVALATDPEIDVFVELIGGEEGVAKDAVEAALRAGKHV
                     VTANKALLAHHGAALAALAEKHGAALNFEAAVAGGIPVVKVIREGLQGNDITRVYGIL
                     NGTCNYILTRMERDGRSFADVLKEAQEQGYAEADPTFDIGGFDTAHKLALLTSLAFST
                     RTKFDAVYVEGIETITPADIEAADDLGYRIKLLGVALRTDSGIEQRVHPTMVPKDTAI
                     ANVDGVSNCVAIDGDYVGDVMLIGPGAGGGPTASAVMSDIIDIARGGILPTFGIKAKH
                     LRPYKKAQMRAHEGGYYIRLSVYDRSGAFAAIAQRMATHGISLESIVQRRPEADPGRK
                     GRRGRDKATVVMVTHETTEAAVRKALAAIERDGH"
BASE COUNT          511 a          849 c          835 g          497 t
ORIGIN      
        1 acaaggagcg gcaggacgaa caaaccggaa agagcgagga cgctccaaaa accaactaaa
       61 atcaaggcga agggtgccgc ttgaagggtt caacagcttg cccggaaggt ccctttcgtg
      121 gttcaactgc agactccctg tttgaggagc cccaatcggg ggcaaatcgc ccgtgcacaa
      181 cgactttcat aggatcaagc ggctgccgcc gtacgtcttt gagaaggtaa accggctcaa
      241 ggccgaagcg cgcgcgcgtg gtgacgacat tatcgacttc ggcatgggca atccggatat
      301 gccgacgccg ccgcacatcg tcgacaagct ggttgagacc ttgcgcgatc cgcggaccag
      361 ccgctattcg gcctccaagg ggatcggcgg tctccgccgg gcccaggccg cgtattacga
      421 ccgccgcttc ggcgtgaagc tcgatccgga cacccaagtc gtggtgaccc tgggctccaa
      481 ggaaggcttc gccaacatgg cgcaggccat taccgcgcct ggcgacgtga tcctcgtgcc
      541 caatccgacc tatccgatcc acgagttcgg tttcatcatg tcgggcggag tgatccggca
      601 cttgcccgcc agcacggatt cccaattcat ggtgtcgctg gacaaggccg tgcgccattc
      661 ggtgccgaag ccgtcggcgc tcgtgctgaa ctatccggcc aacccgaccg ccgtggtcgc
      721 caatcgcaat ttctaccgtg acgtcgtcgc ctacgcgaaa cgcaacgacc tcatcgtgct
      781 gtccgatctt gcttatgccg agatctattt cgatgacgag ccgccgccgt cgatcctcga
      841 aatccccggt gcaatggata tcgccgtgga gttcacgtcc gtttccaaga cgtactccat
      901 gccgggttgg cgcatcggct tcgccgtcgg caacgagcgg ctgatccaag cgttgacgcg
      961 cgtgaagtcc tatctggact atggcgcctt cacacccatt caggtcgcag caactgcggc
     1021 cctcaacggt gagcaggggt gtgtggacga agtgcgcggc gtctatcgcc gtcgccgcga
     1081 ttgcctcgtg gacgcgctcg cacgcgccgg ctggccgatc ccgtcaccac cggccacgat
     1141 gtttgcctgg gcgccgatcc ccgacatgtt cgcgcatgtc ggttcgcttg agttctccaa
     1201 gctgcttctg gagaaggcct ccgtcgcggt ctcacccggc gtcggctttg gtgaatacgg
     1261 cgaaggctat gtgcgcatcg cgcttgtgga gaacgagcag cgtattcgcc aggccgcgcg
     1321 caatattcgt aagttcctca ctgagtcagc gaattcgctg cacaacgtca ttcccatcgc
     1381 ctctaaggca caacggactt tctgatcttt cccgatggcg gatcgtttga agcttggact
     1441 ggccggtatc ggtactgtcg gcggaggtct cttgcggctt ttggaggcgc gcgaggccgt
     1501 tctcgacgcg cgcgcgagcc gtcccatcga agtcgtggcg gtttcggcgc ggagcagacg
     1561 caagaaacgt agcgtcgatc tttcgggtgt gcgtttcgtg gccgatcccg tggcgcttgc
     1621 gaccgatcct gaaatcgacg tcttcgtcga gctgatcggc ggagaggagg gcgtcgccaa
     1681 ggacgccgtc gaggccgcgc tacgcgccgg caaacatgtg gtcaccgcca acaaagcatt
     1741 gcttgcccat cacggggccg ctttggctgc ccttgccgag aagcacggcg cggcactgaa
     1801 cttcgaggcg gccgtcgccg gcggcattcc cgttgtcaag gtgatccgcg aggggctcca
     1861 aggcaacgac atcacccgcg tctacggcat actgaacggc acctgcaatt acatcctgac
     1921 gcgcatggag cgcgacggac gcagcttcgc cgatgtgctg aaggaggcac aagagcaagg
     1981 ctatgcggag gccgatccga cattcgacat tggcggcttc gacaccgcgc acaagttggc
     2041 tcttctgacg agcctcgctt tcagcacacg caccaagttc gatgcggtct atgttgaggg
     2101 gatcgagacc attacaccgg cggacatcga agccgccgac gatcttggct accgcatcaa
     2161 acttctgggt gttgcgctcc gcaccgacag cggcatcgaa cagcgcgtgc atccgaccat
     2221 ggtgccgaag gacacggcca tcgccaatgt cgacggcgtc tccaattgcg tggccatcga
     2281 tggggactac gtcggcgacg tcatgctgat aggccctggc gcgggcggcg gtccgacagc
     2341 ctcggcggtg atgagcgaca ttatcgatat tgcccgcggc ggcatcctgc ccaccttcgg
     2401 catcaaggcg aagcacttaa ggccgtacaa gaaggcgcaa atgcgcgccc atgaaggtgg
     2461 ctactacatc cggctttcgg tctacgaccg ttccggcgcg ttcgccgcga tcgcgcagcg
     2521 catggccacg catgggattt cgctggagag catcgtccag cgccgcccgg aggccgaccc
     2581 gggccgcaaa ggacggcgtg gcagagacaa agccaccgtg gtcatggtca cccatgagac
     2641 gacggaggcc gcagtgcgta aggcattggc cgccatcgag cgggacggcc at
//
LOCUS       DBNHVU010000134        17622 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1983374, whole genome shotgun sequence.
ACCESSION   DBNHVU010000134 DBNHVU010000000
VERSION     DBNHVU010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 17622)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17622)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17622
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1983374"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="AC16Y7_05310"
     CDS             complement(<1..1122)
                     /locus_tag="AC16Y7_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5708830.1"
                     /translation="MTTSERGTLRKWAWRSLLLVAILAAPAAYSEVIPADPGKSKAGT
                     LLGNYLSGRVARGSHDTRAAAEFYSKALAGDPDNEVILEQTFLLETTAARWDRAVKLA
                     KDLVKVEPSHRVAQFLLGVEAFKQREYKQAEEHFAAARQGPIADLTSTLARAWVLEAD
                     GKHNEAFETLDRLSTADWAQYYQRYHRGLIADIAGRHQLARQSYKKAFKKNPATLRVA
                     DAFARHAVNAHDRELAVKTLKAHMSKSAPHPLSEALLAEIDNGGTPPLMATDPTEGLA
                     EVFYGVGDALAGEGGLDMGIVFLQLAIYLKPDFPLAHIALAEAYETAKKYDAEIEAFG
                     HIPEDSPLWINVQIQKAFALNSLEKVDEAKALLEKIIAKS"
     gene            complement(1269..2939)
                     /locus_tag="AC16Y7_05315"
     CDS             complement(1269..2939)
                     /locus_tag="AC16Y7_05315"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HIR5708831.1"
                     /translation="MSESDQVERETMEFDVVIVGAGPAGLAAAIRLKQLAAEKDKEVS
                     VVVLEKGSEVGAHILSGAVIDPVALDALIPDWRDQGAPLTTEVSADHFHYLTSETGGL
                     GLPTIFMPPLMSNHGCYTGSLGNLTRWLGEQAEALGVEVYPGISVTEAIIGAGGEIEG
                     VVTGDLGLARDGSHKDSYTPGMALTGKYTFLAEGARGSLSKELIKKYDLDRESGTQKF
                     GIGLKELWEIPAEKHKPGLVQHTMGWPLEDNTGGGSFLYHFGENLVSVGFVVHLDYAN
                     PYLNPFKEFQRSKLHPRIRQHLEGGKRIAYGARALTEGGWQAIPQLAFPGAALIGCSA
                     GFMNVPRIKGSHNAMLSGMMAAEAAFEAIEAGRAHDTLDAYPQKLKSSALAKELKKVR
                     NVKPLWSRFGTRLGVILGGIDMWLNTLFPFLGHTLKHLKPDYASLDTAAQSKPIDYPK
                     PDNALTFDILSSVFLSSTSHDEDEPVHLKLTDPSIPVDQNLPEYAEPAQRYCPACVYE
                     VVEEGDTVRFQINAQNCVHCKTCDVKDPAQNITWAPPEGGDGPNYANM"
     gene            3105..3905
                     /locus_tag="AC16Y7_05320"
     CDS             3105..3905
                     /locus_tag="AC16Y7_05320"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR5708832.1"
                     /translation="MTGSQAKATADVLAWYEAMGADEAIGEAPVDRFAAPPAPKAKAA
                     PQKPAAARPRPAPNSAKPAASASASMAPESIESAATLEELEALVGQFEGCALKRTAKN
                     TCFYRGSTAARIMLIGEAPGRDEDLQGKPFVGRAGQLLDRMLGAIGLDESSVYITNTI
                     YWRPPGNRTPTPEEIGSCAPFLSRQIELLKPDVMVLLGGAAAKSILDVTDGIMRLRGR
                     WKIYRTETRDIPTLATLHPAYLLRKPGEKRLAWRDMLMLKERLESLKT"
     gene            3902..4459
                     /gene="moaB"
                     /locus_tag="AC16Y7_05325"
     CDS             3902..4459
                     /gene="moaB"
                     /locus_tag="AC16Y7_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017990437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HIR5708833.1"
                     /translation="MTARIDESRPFIPVRIAVLTISDTRTPKTDKSGPLLVSMIKDAG
                     HVAAEHAVAPDEEKKIRKQVRKWIKAKDVDVVITTGGTGFTGRDVTPEAVRPLFDKEI
                     DGFSVLFHMLSYQTVGTSTVQSRACGGLAKDTLIFCVPGSPGACEDAWNGILKFQLDN
                     RHRPCSFIELMPRLTESKKKKAKAS"
     gene            complement(4472..5698)
                     /locus_tag="AC16Y7_05330"
     CDS             complement(4472..5698)
                     /locus_tag="AC16Y7_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HIR5708834.1"
                     /translation="MNIEKMLKPRFGHTVALAALVACTVADNRGVAAEIHGGVHPSPA
                     VILQVLKKFDNPEGAIFSADGSHVFVSNSVEAGDLSKDFGWVEGAGYISKLKVKGNGE
                     LAMAEEKLIDGLTGPLGMGVLPVATKKFPAGTIFLCTGSAPFVDSDGQVVKDPTRMRS
                     KLLAFSEAGKVLGEIDTGQGSVFEKINGSPIILINALGFDADGNLFVTDTAIGAGQFD
                     PPFEAKGGVWMIPHSALDALADGQSPADPPKFLAIPGNPDGVEVSPTNGKIYVNTVGA
                     FASLPDPANGGIYALTKKDIANNNLPPPVDGDLGALDGLDFTAGGVMLNAQIRGDIPG
                     KVTVSCEGQRATTLALEPGGTMSDLKGPADLAIRRSADGAQLVVIPELYARDATAGDD
                     EVTVLALPPEFDAVCP"
     gene            complement(5964..6743)
                     /locus_tag="AC16Y7_05335"
     CDS             complement(5964..6743)
                     /locus_tag="AC16Y7_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding periplasmic protein"
                     /protein_id="HIR5708835.1"
                     /translation="MKRFICCTVFAALAFAGPSAFAADDCTKITATGHPEYPVIAFKE
                     GDNIAGAAPDLVAAIAKKLNVPLQSKDMGSWEEAQEATRDGKADLIVGVYYNDARAGY
                     LDYVKPAFMYDDVAVFVAKGKKFPFKGKDDLIGKKGVTSEGESYGNDFDAFMNDKLDV
                     TRTKGIGAAFKDLLAGKVDYLIAGYHPGVAEAADKGLKDQVVVLDQALLTAEMFVAFS
                     KKSPCRSLAADFGHGITTLTTDGSFDKMLTDATAEWDKTQQ"
     gene            complement(6756..7052)
                     /locus_tag="AC16Y7_05340"
     CDS             complement(6756..7052)
                     /locus_tag="AC16Y7_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708836.1"
                     /translation="MTGYRAAEVAVFVLVLAFACMPASPAWSQAGTITKGMQNNCVND
                     YKKFCGEYGLQTSALNYCMKKAGPSLSPACVRALVQAGKISQAEVDRIKAQMGR"
     gene            7211..7381
                     /locus_tag="AC16Y7_05345"
     CDS             7211..7381
                     /locus_tag="AC16Y7_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HIR5708837.1"
                     /translation="MNQALVKLITDESGATAVEYGLIAAGIAVAISSSLSLVGCNLAA
                     LFTNVANEVKAR"
     gene            complement(7310..7963)
                     /locus_tag="AC16Y7_05350"
     CDS             complement(7310..7963)
                     /locus_tag="AC16Y7_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR5708838.1"
                     /translation="MAKRPQAGAVKRRLARDIGVGLATRFYRNALARTVQRLAADPRW
                     RTYLAVTPDAALTEACWPRVANLTLIPQGRGDLGQRMQSLFDRLPPGPVVVVGSDIPA
                     VRPAHIAQSFKLLGAADAVFGPAEDGGYWLAGQKRCPKGLAPFANVPWSTDDALAATL
                     ANLRGRSVAFAATLRDVDTGEDYARERHRLGFKASAGFDFVGDIGEQRGQVAADQRQ"
     gene            complement(8010..8699)
                     /locus_tag="AC16Y7_05355"
     CDS             complement(8010..8699)
                     /locus_tag="AC16Y7_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR5708839.1"
                     /translation="MISVVIPTFNAAPTLVHTLAALVPAVVEGIVQEAIVADGGSTDD
                     TLVIADAAGTHLVQAPRGRGTQLDVGAGLAKGDWLLFLHADTVLEPGWAEEAQSFIDR
                     VEAGRRRQAAAYFRFCLDDDGFMPRLVEGLVGLRCFLLALPYGDQGLLISRKLYDELG
                     GFRAVPLMEDVDLVRRLKRRQLVALKSRAVTSAQRYQAEGYFSRGVRNLGLMLLYYLR
                     VPPRVLARLYG"
     gene            8789..9910
                     /locus_tag="AC16Y7_05360"
     CDS             8789..9910
                     /locus_tag="AC16Y7_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007814287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HIR5708840.1"
                     /translation="MSVALRKQVDSESLPAEAGGVAFDRRHGRGAQTNRSGRFENLAY
                     EPADDGWDSLFDLEALQTEVQEMPARQIITKNQSPDIGFDRSINPYRGCEHGCVYCFA
                     RPSHAFLGMSPGLDFETKLFAKTNAARALEKELAAPNYRPRMIAIGSNTDPYQPVERR
                     YRIMRRILEVLSAANHPVGIVTKSALVLRDLDILQSMAKRGLVKVALSVTTLDRKLAR
                     AMEPRAATPEKRLETVEKLASAGVPAAVMVAPVIPGLTDSEMERILERASVAGAAEAG
                     YILLRLPLEIGDLFVEWLKANCPDRAERVLSLMRQSRGGKLYEAKFGDRMEGHGPYAW
                     MIGRRFQMAAQRLGLNGSVPLRTDLFTPPKRPGQQLRLL"
     gene            9988..10674
                     /locus_tag="AC16Y7_05365"
     CDS             9988..10674
                     /locus_tag="AC16Y7_05365"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR5708841.1"
                     /translation="MALPAEPLPAPSLPSFDLEEELMDLHGLPVAGIDEAGRGPWAGP
                     VVVAAVILNPNRIPEGLNDSKVLTPQAREDRFAEIIATSCVSITIGQVRRIDRDNILQ
                     ASLWGMRAAFRGLQVPQAAALIDGNLVPKRFPGGKARAIVGGDALSLSVAAASIIAKV
                     TRDRIMVKLAKRYRRYGWESNKGYGTPEHARAVREHGVCTHHRRSFAPIERALRIMNE
                     RDEASPVAET"
     gene            complement(10671..11423)
                     /locus_tag="AC16Y7_05370"
     CDS             complement(10671..11423)
                     /locus_tag="AC16Y7_05370"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5708842.1"
                     /translation="MSQTIVMIHGANCGGWCFGVFAKVFEANGFTCHAPDLIGHGAEK
                     ETGRGTLANVGIADFRAQMTTFVKLLPEKPILLGHSMGAVIAQQLASEDLAEKLVLVS
                     PAPRAGILPPTAAEKQLCQDLMTIGDFWTRAIDPNFELACHYTLNLLPQDAQRRVFDE
                     FGPESGRALFEFFFWMFDQTVATEVDTSAVTCPVLCLSGAEDHIVSLQSAKATAEAYR
                     QAEFWALQTHAHMLLVEPGADKIAECIGGWLA"
     gene            11543..12640
                     /locus_tag="AC16Y7_05375"
     CDS             11543..12640
                     /locus_tag="AC16Y7_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004448152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HIR5708843.1"
                     /translation="MGVGDTGELRLPRNKIIVGDCIEELARLPEGSVDLVFADPPYNL
                     QLANTLLRPNNSRVDGVDDDWDKFSSFGAYDAFTRDWLTACRRVLKPNGSLWVIGSYH
                     NIFRVGTALQDLDYWVLNDVIWRKANPMPNFRGRRFTNAHETMIWASTGQKARYTFNY
                     ESMKALNEDLQMRSDWLLPICSGGERLKQDGRKTHPTQKPEALLHRVILASSHPGDVV
                     LDPFMGSGTTAVAAKRLGRTFVGIEREEAYANAARTRLKGVKPLDAAALQVSKSKRAE
                     TRVPFGTLLELGLLAPGAALYDPAARHEAKVRADGSIACDGAQGSIHKIGAHVQGAMA
                     CNGWTFWHYQADGALKPIDDLRTAARAKLAS"
     gene            complement(12588..13775)
                     /gene="mutY"
                     /locus_tag="AC16Y7_05380"
     CDS             complement(12588..13775)
                     /gene="mutY"
                     /locus_tag="AC16Y7_05380"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIR5708844.1"
                     /translation="MQESHMALEPRAIERAVLAWYDREARALPWRAPPKERQDPYKVW
                     LSEIMLQQTTVKAVAPRYIDFLRRWPDVAALARAELGEVLAAWAGLGYYARARNLHAC
                     ARAVAGEHGGDFPDTEAELRKLPGIGAYTAAAIAAIAFGKRATAVDGNIERVMARLFA
                     VERPLPAAKKELKALAESLTPETRAGDFAQSLMDLGATICTPRRPACGLCPVRPACRG
                     YAQGVAEQLPYKEEKAPRPTRRGVAFVVLREDGAVLLRTRPLKGLLGGMLEVPSTPWE
                     ETAPTKTARAAHAPVDLPWQDVSGRVEHTFTHFHLELCVVRAVVPEGVALLEVAEPER
                     CRWLTMRELPGAALPTVMRKVLAHAFEAKAKRRAPKKQASKTRASKTRASPVRRSEDR
                     RSA"
     gene            13880..14398
                     /locus_tag="AC16Y7_05385"
     CDS             13880..14398
                     /locus_tag="AC16Y7_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HIR5708845.1"
                     /translation="MARQTRKPRYGARAIGSYVARALDPAARARGFATTALLSDWPAI
                     VGRDLARFTMPDRVIWPRRRDEEAETPARGHKAEGATLVLRIEGPRAIEVQHRSGQIL
                     ERVNGYFGYRAVTEMRFLQAPIARVRKTPPAKRPPLPAYSLPKTVDIEDEGLAQALTK
                     LGAATKARLTRA"
     gene            14545..15276
                     /locus_tag="AC16Y7_05390"
     CDS             14545..15276
                     /locus_tag="AC16Y7_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019169797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR5708846.1"
                     /translation="MAKPKKPTEPSTEDTKINKTGLIVGGAVLAAVVVAGIVLFDDRG
                     GAPDPNAPKMDPEMAALMEAGPLEDIVLGNADAPFVIVEYASMTCPHCANFYTEVFPQ
                     VKEKYIDTGKARFIFREFPLDGLAVAASMLVRCSGNDRFYPMLDGLFETQETWAAPGE
                     DGKEKLLMIAKQAGFSQEAFDKCLADKELFDKIVAVRKKAHEEYGVDATPSFFVNGKR
                     LKGIGIDKFEAAIEGTGKPDTPPSG"
     gene            15291..15929
                     /locus_tag="AC16Y7_05395"
     CDS             15291..15929
                     /locus_tag="AC16Y7_05395"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR5708847.1"
                     /translation="MGRVRGAALVVIAALGLSGCGGGAGTPTEAAVPLKEEMVQPGPL
                     GDRVYGNKNAPVTIIEYVSLTCPYCRQYHAKVFPKVKRQYIDTGKVRYIIREFPIGRT
                     AGNAAIVTACVSKSKYLPLVEAFLSRQREWVSQEVRPNAIYSVAKSSGMSRAEFDKCL
                     SNQSIIDGLTVVKERGRKYGVIGTPSFFINGQKLQGTVTFEEIKALIQPQAS"
     gene            16031..>17622
                     /locus_tag="AC16Y7_05400"
     CDS             16031..>17622
                     /locus_tag="AC16Y7_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5708848.1"
                     /translation="MQITRLRLLGFKSFVEPTELLVETGLTGVVGPNGCGKSNLLEAL
                     RWAMGETSYKSMRGSAMDDVIFAGSQDRPARNTAEVTLFLDNSKRLAPAEFNDSDLLE
                     VTRRIEREAGSAYKINGKDVRARDIRLLFEDAATGARSPALVQQGRIGEIVNAAPQER
                     RRVLEDAAGIAGLHSRRHEAELRLKAAETNLGRLADLIGQIKSQLNAMRRQARQAKRY
                     RQISDKIHEMEALQYHLQWTAACGQVEADEAAFQESLSAVAEETKAEGEALRRQAEAA
                     EKLQPLRDAEAERAAVLHRLSVERDALEREEARAKARQAELETRLAQASRDLAREEEH
                     IAEAESMLRGLGEESRKLVASSERDEEEPQLRAAAAKAVEARIAAEDALRQATTELAE
                     AKAERGRLEGEKTTTAARIAQLESQAGDVEQELAKLEAETGGSAQFAELRADADRLTA
                     ALQETETALAKAEADEEQARAEEARLRSDAAAAKLSRQEIETEFATLTKLLAPAHDWT
                     PIVEEVSVEGGYEQALGAALGD"
BASE COUNT         3294 a         5694 c         5575 g         3059 t
ORIGIN      
        1 gctcttcgcg atgattttct caaggagcgc cttggcttcg tcgacttttt ccagggagtt
       61 gagtgcgaac gccttctgga tctgtacgtt gatccaaagc ggcgagtcct ccgggatatg
      121 accgaaagcc tcgatctcag cgtcgtactt cttggccgtc tcataggctt cggcgagcgc
      181 gatgtgggcc agcgggaaat cgggcttcag atagattgcg agttggagaa acacgatccc
      241 catgtcgaga ccgccctcgc ccgcaagcgc atcgccgacg ccgtagaaca cttcggcgag
      301 accctctgtc ggatcggttg ccatcaacgg cggcgttccg ccgttatcga tctcggcgag
      361 caatgcttca gacaagggat gcggcgcact ctttgacatg tgcgccttga gcgttttgac
      421 cgcgagttcg cgatcgtgcg cgttcacagc gtgacgcgca aaggcatcgg ccactctgag
      481 cgtggctggg tttttcttga aagctttctt gtaggactga cgcgcaagct ggtggcgtcc
      541 agcgatgtcg gcgatgagcc cccggtggta gcgctggtag tactgggccc aatcggcggt
      601 gctgagacga tccagcgtct cgaaggcttc gttgtgcttg ccgtccgctt ccaagaccca
      661 ggcacgcgca agcgttgacg tcaggtcggc gatgggccct tggcgggcgg cggcgaagtg
      721 ctcttcggct tgtttgtact cgcgctgctt gaaagcttcg acgcccagca agaactgagc
      781 gactcgatga gacggctcga ccttgaccaa atccttggcg agcttgacgg cgcgatccca
      841 gcgcgccgcg gtggtctcca gcaggaaggt ctgctccagg atgacttcgt tgtcgggatc
      901 gccggcaagc gccttggagt agaactcggc cgccgcacgc gtatcgtgac tgccgcgcgc
      961 gacacggccc gacaggtaat tgccaagcaa cgtcccggcc ttggacttac cgggatccgc
     1021 cgggatcact tcggaatagg cggcaggcgc tgcaaggatt gcgacgagga ggagagacct
     1081 ccacgcccat ttgcgcagcg tccctctttc agaagttgtc atcttgtcgg tccggctatc
     1141 cgccgcccca aattgttgtg tgccgtggcc aaaggtatgg gcttttccca gccctgcaat
     1201 agcttttttg agggccaatc gcggcgcgtt tatggcctca aaacgggagg ccaacaggga
     1261 tttcagggtc acatattcgc gtagttgggc ccgtccccgc cttccggagg tgcccaggtg
     1321 atattctggg ccgggtcctt cacgtcgcag gttttgcagt ggacgcaatt ctgcgcgttg
     1381 atctgaaagc ggaccgtgtc gccctcctcc actacctcgt agacgcaagc cgggcagtag
     1441 cgctgcgccg gctcggcata ctcgggcagg ttctggtcca ccgggatcga ggggtcggtg
     1501 agcttcagat gcaccggctc gtcctcgtcg tgactggtgc tggacaagaa caccgacgac
     1561 agaatgtcga aggtgagtgc gttgtccggc ttcggataat cgatcggttt ggactgcgcc
     1621 gccgtgtcga ggctcgcata gtccggcttc aaatgtttga gagtgtgacc gagaaacgga
     1681 aacagcgtgt tcaaccacat atcgatgccg ccaagtataa cgccgagccg cgtcccaaag
     1741 cgcgaccaca gaggcttcac gttgcggacc ttcttcaact ccttggcaag cgcgctcgac
     1801 ttgagcttct gaggataggc atccagcgta tcgtgcgccc ttcccgcttc gatcgcttcg
     1861 aatgccgctt ccgccgccat cattccggac agcatggcgt tgtgcgagcc cttgatgcgc
     1921 ggcacattca taaagcccgc gctgcatccg atcagcgccg cccccggaaa ggccagctgc
     1981 gggattgcct gccagccgcc ttcggtcagc gcgcgtgcac cgtaggcaat gcgcttgccg
     2041 ccttccaaat gctggcgaat gcgcgggtgc agcttggagc gctgaaactc cttgaacggg
     2101 ttgagatacg gattggcgta atcgagatgc acgacgaagc cgaccgacac caggttctcg
     2161 ccaaaatgat aaaggaacga cccgccgccg gtattgtcct caagcggcca gcccatggtg
     2221 tgctggacga gaccaggttt gtgcttctct gccgggatct cccaaagttc tttcaggccg
     2281 atgccgaatt tctgcgtgcc gctctcacgg tccaggtcgt acttcttgat gagctctttg
     2341 ctgagagaac cacgcgcgcc ctcggcaaga aacgtgtact tgccggtcag cgccatgccg
     2401 ggcgtatagc tgtccttgtg gctgccatcg cgcgccagac cgagatcgcc ggtgacgacg
     2461 ccttcgatct cgccgccggc gccgatgatc gcctcggtca cggagatgcc cggatagacc
     2521 tcgacgccca aagcttccgc ttgctcgcca agccagcggg tgaggttgcc gaggcttccc
     2581 gtgtagcaac cgtgattgct catgagcggc ggcataaaaa tcgtggggag accgaggccg
     2641 cccgtctccg acgtcagata atggaaatgg tccgcgctca cctcggtggt gagcggcgcg
     2701 ccctggtcgc gccaatcggg gatgagcgca tcgagggcaa cagggtctat cacggcgccg
     2761 gacagaatat gggcgcccac ctcggagccc ttctccagaa cgacgacgga aacctctttg
     2821 tccttctcgg cggcgagctg tttgaggcgg attgcggcgg cgaggcctgc gggccctgcg
     2881 ccgacgatca ccacatcgaa ctccatggtc tcgcgttcga cctgatcgct ctcgctcatc
     2941 tcgccccttt cgttccaaag gcttttttgg ttcaaacctc cgccgctctg cccgctccgc
     3001 cccgcaggca cagcaaatcg gtgctaaatt gaagccaccc agcttcacgc gatggcccgg
     3061 tgggccgggc aatgacaaat agaggatcgg cacgggcatc gcaaatgact ggaagccaag
     3121 caaaagcaac cgcggacgtg ctggcctggt acgaggccat gggcgccgac gaggccattg
     3181 gcgaggctcc ggtcgaccgc ttcgccgcac cgccagcgcc aaaggccaag gcagcgcccc
     3241 aaaagccggc cgcggcgcgt ccccgcccgg cccctaactc agcgaagccc gccgcatcgg
     3301 cttcggcatc gatggcgccc gaatccattg aatcggcggc gacgctcgaa gagctggagg
     3361 cgcttgtcgg ccagttcgaa ggttgcgccc tgaagcgcac cgccaagaac acctgctttt
     3421 accgcggcag cacggcagct cgcatcatgc tgatcggcga ggcgccggga cgcgacgagg
     3481 acctgcaagg caaacccttt gtcgggcgcg cagggcaatt gctcgaccgc atgcttggcg
     3541 ccatcggcct cgacgagagc tccgtctaca tcaccaacac gatctattgg cgcccgccag
     3601 gcaatcgcac gccgacgcct gaagagatcg gctcttgcgc gccgttcctg tcgcggcaga
     3661 tcgagctgct caagcccgac gtcatggtgc tgctcggcgg cgcggcggcg aagtccattc
     3721 tcgacgtcac cgacggcatc atgcgcttgc gcgggcggtg gaagatctac agaacggaga
     3781 cccgcgacat tcccacgctc gcgacgctgc acccggccta tctgctccgc aaaccgggcg
     3841 agaagcggct tgcatggcgc gacatgctga tgctgaagga aagactggag agtttgaaga
     3901 catgaccgcg cgtatcgacg agagccgccc attcatcccc gtgcgcatcg ccgtgctcac
     3961 catctccgac acgcgcacgc ccaagacgga caagtcgggt ccgctgctgg tcagcatgat
     4021 caaggatgca ggacacgttg cggcagagca cgccgtcgca ccggacgaag agaagaagat
     4081 ccgcaagcag gtgagaaagt ggatcaaggc gaaagacgtc gatgtcgtga taaccacggg
     4141 cggcaccggc ttcaccgggc gcgacgtgac gccggaagcc gtgcgcccgc tattcgacaa
     4201 ggagatcgac ggcttctccg tgctgttcca catgctgagc tatcagacgg tcggcacgtc
     4261 cacggtgcaa tcgcgcgctt gcggcggcct cgccaaagac acgctcatct tctgcgtgcc
     4321 gggctcaccg ggcgcttgcg aggacgcctg gaacggcatc ctcaaattcc agttggataa
     4381 ccgccaccgg ccctgctcgt tcatcgagct gatgccgcgc cttaccgaga gcaaaaagaa
     4441 gaaggcgaaa gccagttaag gccgcgacac atcacgggca gacggcatcg aattcgggcg
     4501 gcaacgcgag cacggtgacc tcgtcgtcac cggcggtcgc gtcgcgagcg tagagctcgg
     4561 gaatcaccac cagctgtgcc ccatcggcgc tgcgccggat cgccaggtcg gcgggcccct
     4621 ttaggtcgga catggtgccg cccggctcaa gcgcgagcgt ggtggctctt tggccttcgc
     4681 agctgactgt cactttgccg ggaatgtcgc cacgaatctg ggcgttcagc atcacgccgc
     4741 cagcggtgaa gtcgaggcca tcgagggcac ccaggtcgcc gtcgactggc ggcggcagat
     4801 tgttgttggc gatgtccttc ttggtcaggg cgtagatgcc gccgtttgcc gggtcgggca
     4861 agctcgcgaa tgccccaacg gtgttgacgt agatcttgcc gttcgtgggc gagacctcga
     4921 ccccgtcagg gtttccgggg atcgcgagga acttcggcgg atcggccgga gactgaccgt
     4981 ccgcaagagc gtcaagcgcg ctgtgcggga tcatccagac gccacccttc gcctcgaacg
     5041 gcggatcgaa ctgacctgcc ccgatcgcgg tgtcggtgac aaagaggttg ccatcggcgt
     5101 cgaaccccag cgcgttgatc aaaatgatcg ggctaccgtt gatcttctcg aacaccgatc
     5161 cctggccagt gtcgatctcg ccgagcacct tgccggcttc gctaaacgct agaagcttac
     5221 tgcgcatgcg ggttgggtcc ttgaccacct gaccgtcgct atcgacgaac ggtgccgagc
     5281 cggtgcacag aaagatggta ccggccggga acttcttcgt agccactggc aggacgccca
     5341 tgccgagcgg cccggtcagg ccgtcgatca acttctcctc ggccatcgcc agctcaccgt
     5401 tgcccttgac cttcagctta ctgatatacc ccgcgccctc gacccaacca aagtctttgg
     5461 acagatcgcc ggcctcgacg gagttggaaa caaagacatg gctgccatcg gcgctgaaga
     5521 tcgcgccttc cggattgtca aatttcttga gcacctgaag gatgaccgcg ggagacgggt
     5581 ggaccccgcc gtggatctcc gccgcaacgc cgcgattgtc ggccacggtg catgcgacca
     5641 gagccgcgag ggcgactgta tgcccgaagc gcggcttcaa cattttttcg atgttcattg
     5701 aaccaatcct tcaactcgta gatggagccg cgctagttca tgcgaacgat gtcgggccgg
     5761 ttggggcgtc taggcgaccg cttgaagact gcccgaggcc ataatcgaca accgccacca
     5821 cgctattacg attgttggtt gcagtcgact cccctttagc ccgggagcaa tcgacggcgg
     5881 tttttgcaag caatcacaca gccgctgacc acgacgcgat catgcgtcta gtcgcgaatg
     5941 agggcgcctt cgatatggcc cgcctattgc tgggtcttgt cccactccgc tgtcgcgtct
     6001 gtcagcatct tgtcgaagct gccatcggtg gtcagcgtgg tgatgccgtg accgaagtct
     6061 gcggctaatg agcggcacgg cgacttcttc gagaaggcga cgaacatctc cgccgtgagc
     6121 aaggcctgat cgagcacgac gacctgatcc ttcagcccct tgtcggccgc ctcggcgacg
     6181 ccgggatgat atccggcgat caggtagtca acctttccgg ctaagagatc tttgaacgcc
     6241 gcgccgatcc cctttgtacg ggtgacgtcg agcttgtcgt tcatgaaggc atcgaagtcg
     6301 ttgccgtaac tctcgccctc gctcgtcacg cccttcttcc cgatcaggtc atcttttcct
     6361 ttgaagggga acttcttccc cttggcgaca aatacggcca cgtcgtcata cataaaggcg
     6421 ggcttgacat agtcgagata gccggcgcgg gcatcgttgt agtagacgcc gacgatgaga
     6481 tcagccttgc cgtcgcgcgt tgcttcttgc gcttcttccc acgaccccat gtctttcgac
     6541 tgaagcggga cattgagctt cttggcgatg gccgcgacaa ggtcgggtgc ggcgccggcg
     6601 atgttgtcac cctctttgaa ggcgatcacc ggatactcgg gatgacccgt tgccgtaatc
     6661 ttggtgcaat cgtccgcagc aaaggcgctg ggccctgcga acgcgagcgc ggcgaaaacg
     6721 gtgcagcaga taaatctctt catgatccag atcccctaac gtcccatctg cgccttgatg
     6781 cgatcgacct cggcttgcga aatcttgccg gcctggacca gagcacgcac gcaagcaggt
     6841 gagaggctcg ggcctgcttt cttcatgcaa taattcagag cagacgtttg aagtccgtac
     6901 tcaccacaga atttcttgta gtcgttgacg cagttgttct gcatgccctt tgtgatggtg
     6961 ccagcctgtg accacgccgg agacgccggc atgcaggcga atgcaagaac cagaacgaag
     7021 actgcgacct ccgccgcgcg ataaccggtc atccctcaac ccctccaaag ttctacgaat
     7081 ctctatagca cacgccgcgc gaccgatctt ccttatatgc acaggccaat tcggggccgg
     7141 gcgcgcacct taacttgact taaggtattg ctgtgtaagt agaaagttcc aagactggag
     7201 cactgccgca atgaatcagg ctctcgtgaa gctcatcacg gacgagtccg gcgccacggc
     7261 cgtcgaatat ggccttatcg ccgccggcat cgccgttgcg atctcctcgt cactgtcgtt
     7321 ggtcggctgc aacctggccg cgctgttcac caatgtcgcc aacgaagtca aagcccgctg
     7381 acgctttaaa gccgaggcgg tgccgctcgc gcgcgtaatc ctcccccgta tcgacgtccc
     7441 tgagcgttgc ggcgaaggcg acgctccgcc cacgcaagtt cgcgagtgta gcagcgagcg
     7501 catcgtccgt ggaccaaggc acgttggcga agggcgctaa gcctttgggg catcttttct
     7561 gtcctgccag ccaatagcct ccgtcctcgg ccggtccgaa caccgcatcc gcggccccca
     7621 gcaatttgaa gctttgcgcg atatgggcgg gccgcaccgc gggaatgtcg ctacccacca
     7681 cgacgacagg ccccggcggc aggcgatcga acaggctttg catgcgctgg ccgagatcgc
     7741 cgcggccttg tggaatgagc gtgaggttcg caacgcgcgg ccagcacgct tctgtaagcg
     7801 cggcgtcggg cgtgacggca agataagtgc gccagcgcgg atcggccgcc aaacgttgga
     7861 cagtgcgagc aagcgcgttg cggtagaagc gggtcgcgag gcccactcca atgtcgcgcg
     7921 ccagccggcg cttcacggcg cccgcctgag ggcgcttggc catgatgaca agacggggtg
     7981 cgaaagagat gcgggcgtct cgccgctgcc taaccataaa ggcgggcgag cacgcgcggc
     8041 ggcacgcgaa ggtaatagag cagcatgagg ccgaggttac ggacaccgcg cgagaagtaa
     8101 ccttcggcct gatagcgctg ggcgctggtc accgcgcgcg acttcagagc gacgagctgg
     8161 cgccgcttga gacggcggac gagatccacg tcctccatca ggggaacggc gcgaaagccg
     8221 ccgagctcgt catagagttt gcgggaaata agcaggccct gatcgccata aggcagcgcg
     8281 agcaggaaac agcgcaagcc caccaggcct tcgaccaggc gcggcatgaa gccgtcgtcg
     8341 tcgaggcaga agcggaaata agccgctgcc tgccggcggc gcccggcctc cacgcggtcg
     8401 atgaagcttt gcgcctcttc cgcccagccc ggctcaagca ccgtgtcggc atgcagaaac
     8461 agcagccagt cgcccttcgc gagccccgcg cccacgtcga gctgcgtgcc ccgcccgcgc
     8521 ggcgcctgca ccagatgcgt gccggcggcg tcggcgatca ccagggtgtc gtcggtcgag
     8581 ccgccatcgg cgacgatggc ctcctgcacg atgccctcca ccacggccgg cacgagggcc
     8641 gccagcgtat ggaccaatgt gggcgccgca ttgaaggtgg gaatgaccac ggaaatcatg
     8701 ggcggactat agcgtttggc ctgcttgcgg caatcgcagg ctgcccgttg ttcttgcttt
     8761 gttctctccg ccgggctata taggccccat gagcgttgcc ttacgaaaac aggtcgattc
     8821 ggagagcctg ccggccgaag ccggcggcgt cgccttcgac cggcggcatg gccggggcgc
     8881 ccagacaaac cggtcggggc ggtttgagaa cctcgcctac gagcccgccg atgacggctg
     8941 ggactctctt ttcgatctcg aagcgcttca gaccgaagtc caggagatgc cggcccggca
     9001 gatcatcacc aagaaccagt cgcccgatat cggcttcgac cgctcgatca atccctatcg
     9061 gggctgcgag catggctgcg tctattgctt cgcccgcccg agccacgcat ttctcggcat
     9121 gtcgcccggg ctcgatttcg agaccaagct gtttgccaag accaatgcgg cgcgggcgct
     9181 ggagaaggag ttggccgcac cgaactaccg cccccgcatg attgccatcg gctcgaacac
     9241 cgatccctat cagccggtgg agcggcgcta ccgcatcatg cggcgtattc tcgaagttct
     9301 gagcgccgcc aatcatccgg tcgggatcgt caccaagtcg gctctggtgt tgcgcgatct
     9361 cgatatcttg cagtcgatgg cgaagcgcgg cctggtcaag gtggcgctgt cggtgaccac
     9421 gctcgaccgt aagctcgccc gcgccatgga gccccgcgcc gccacccctg agaaacgcct
     9481 tgagaccgtc gagaaactgg caagcgccgg cgtgcccgcc gccgtcatgg tggcgccggt
     9541 cattccggga ctgaccgatt cggagatgga gcgcatcttg gagcgcgctt ccgtcgcggg
     9601 cgcggccgag gcgggctaca tcttgctgcg gctgccgctg gagatcggcg atctcttcgt
     9661 ggagtggctc aaggccaatt gtccggaccg cgccgagcgc gtgttgtcgc tgatgcgcca
     9721 gagccgcggc ggcaagctgt acgaggcgaa attcggcgac cgcatggaag gccacgggcc
     9781 ttacgcctgg atgatcggcc gccggtttca gatggccgcc cagcggctcg gtctcaacgg
     9841 ctccgttccc ttacggaccg atctgttcac tccgccaaaa cggcccggcc agcagctgcg
     9901 cctcttgtag gtttttccac agcgcccggc acttaagggc cggggtagtt gcgcggccgg
     9961 cgcggtttgt cggaaagtcg ccggatcatg gcgctgcccg cagaaccgct tcccgcccct
    10021 tctctcccga gcttcgatct cgaagaagag ctcatggatc tgcacgggct gcccgtggcc
    10081 ggcatcgacg aggcgggccg cggaccctgg gcgggacctg ttgtggtcgc ggcggtgatc
    10141 ctcaatccga accgtattcc cgaagggctc aacgactcca aagtgctgac accccaggcc
    10201 cgcgaggacc gcttcgcgga gatcatcgcc acgtcgtgcg tgtccatcac catcggccag
    10261 gtgagacgca tcgaccgcga caacattttg caggcgagcc tgtggggcat gcgcgccgcg
    10321 ttccgtgggc tacaggtgcc gcaagccgca gcactgatcg acggcaatct ggtgccgaag
    10381 cgctttcccg gcggcaaggc gcgcgcgatc gtcggcggcg acgcgttgtc gttgtcggtg
    10441 gcggccgcct cgattatcgc caaagtcacc cgcgaccgga tcatggtgaa gctcgccaag
    10501 cgctatcgcc gctatggctg ggaaagcaat aaaggctatg gcacgccgga gcatgcccgc
    10561 gcggtgcgtg agcacggcgt gtgcacgcat caccgcagat cgttcgcgcc gatcgagcgt
    10621 gcgttgcgca tcatgaacga gcgcgacgaa gcttcgccgg tcgccgagac ttaagcgagc
    10681 cagccgccga tgcactcggc gattttgtcg gcgccaggct ccaccagcaa catatgcgcg
    10741 tgggtttgaa gcgcccagaa ctcggcttgg cggtaagcct ccgccgtcgc cttcgcgctc
    10801 tgcaacgaca cgatgtgatc ctccgcgccc gataggcaca gcaccggaca ggtcaccgcg
    10861 gacgtatcga cttccgtcgc cacggtctgg tcgaacatcc agaagaagaa ctcgaacagc
    10921 gcgcgcccgg attccgggcc gaactcgtcg aacacgcgcc gctgcgcgtc ctgcggcaag
    10981 aggttcagcg tgtagtggca agccagttcg aagttcggat cgatggcacg cgtccagaaa
    11041 tcgccgatgg tcatcaagtc ctggcagagc tgcttctctg cggccgtcgg cggcaagatg
    11101 ccggcgcgcg gcgccgggct caccagaacg agcttctcgg cgagatcttc gctcgcaagc
    11161 tgctgggcga tcaccgcacc catggaatgg ccgagcagga tgggcttctc cggcagcagt
    11221 ttgacgaatg tggtcatctg cgcacggaaa tcggcgatcc cgacattggc caaggtgccc
    11281 cgccccgtct ccttttcggc cccatgtccg atcaggtcgg gcgcatggca ggtgaagcca
    11341 tttgcctcga aaaccttggc aaagacgccg aaacaccagc cgccgcaatt ggcgccgtgg
    11401 atcatgacga tggtctggct cacttgcggg tctctccctc ggcttatcgg ttaaggacgc
    11461 ctttaccgta ctccgccagc atgggccggt cctgcccgaa agaggcgggg ttttcagtat
    11521 ttgcgtaacg agagtgggtg acatgggtgt gggggacact ggggagcttc ggcttccccg
    11581 taacaaaatc attgtgggcg attgcatcga ggagctggcg cggcttccgg aaggctccgt
    11641 cgatctcgtc ttcgccgacc cgccgtacaa ccttcagctc gccaacacgc tgctgcggcc
    11701 gaacaatagc cgcgtagacg gcgtggacga cgactgggac aagttctcca gcttcggcgc
    11761 ctatgacgcc ttcacccgcg attggctcac cgcctgccgc cgcgtattga agccgaatgg
    11821 cagcctctgg gtgatcggca gctatcacaa tatctttcgc gtcgggacgg cgcttcagga
    11881 cctggactac tgggtgctga acgacgtgat ctggcgcaag gcgaacccga tgccgaattt
    11941 ccgggggcgc cgcttcacca acgcgcacga gaccatgatc tgggcctcga caggccagaa
    12001 ggcgcgctac acgttcaact acgagtctat gaaggcgctg aacgaagatt tgcagatgcg
    12061 ctccgattgg ctactgccga tctgctccgg gggcgagcgt ctgaaacaag acggacgcaa
    12121 gacgcacccg acgcaaaagc ccgaggcgct gctgcaccgc gtgatcctcg catccagcca
    12181 tccgggcgac gtggtgctcg acccgttcat gggctccggc acgacggcgg ttgcggcgaa
    12241 gcggttgggg cgcacctttg tcggcatcga gcgcgaagag gcgtacgcga acgccgcgcg
    12301 cacaaggctg aagggcgtga agccgctcga tgcggcggcg ctgcaagtct ccaagagcaa
    12361 gcgcgcggaa acacgggttc ccttcggcac actgcttgag ctcggcctgc tggcgccggg
    12421 tgcggcgctt tacgatccgg ccgcgcgtca cgaggcgaag gtccgtgctg acggctccat
    12481 cgcctgcgac ggcgcacagg gctccattca caagatcggt gcccatgtgc aaggcgccat
    12541 ggcctgtaat ggctggacct tctggcacta ccaagcggac ggcgcgctca agccgatcga
    12601 cgatcttcgg accgccgcac gggcgaagct cgcgtcttag aggctcgcgt cttagacgct
    12661 tgcttcttag gggcgcgcct cttcgccttc gcttcgaagg cgtgggccag cactttgcgc
    12721 atcacggttg gtagcgcggc accaggcagt tcgcgcatgg tgagccagcg gcagcgctca
    12781 ggttccgcca cctctaacag cgcgacaccc tccggcacca ctgcgcggac aacgcaaagt
    12841 tccaagtgaa aatgcgtgaa cgtgtgttcg acgcggccgg acacgtcttg ccacggcaga
    12901 tcgacgggcg cgtgggctgc gcgcgccgtt ttggtcggcg ccgtctcctc ccaaggcgtt
    12961 gacggcactt ccagcatgcc gccgagcaaa cctttcagcg ggcgtgtccg cagcaggact
    13021 gcgccgtcct cgcgcaacac cacgaacgcc acgccacgac gcgtcggccg gggcgccttc
    13081 tcttctttat agggaagctg ttcggcaacc ccttgtgcgt agccacggca ggcggggcgg
    13141 accgggcaaa gcccgcaagc aggacgccgc ggcgtgcaga tggtggcgcc gagatccatt
    13201 aggctttgcg cgaaatcgcc ggcgcgggtc tcgggcgtca aactttcggc gagcgccttg
    13261 agttccttct tggcggcggg cagcggcctc tccaccgcga acaaccgcgc catgacgcgc
    13321 tcaatattac cgtcgacggc cgtggcgcgt ttcccgaagg caatcgcggc aatggccgcg
    13381 gccgtgtagg cgccaatgcc ggggagcttg cgaagctccg cctccgtatc gggaaagtcc
    13441 ccgccatgtt cgcccgccac ggctcgcgcg cacgcatgga gatttcgtgc gcgggcgtaa
    13501 tagccaaggc cggcccaagc ggcgagcacc tcgccgagct cggcgcgggc aagcgcggcg
    13561 acgtcgggcc agcggcgcag gaaatcgata tagcgcggcg cgacggcttt caccgtggtc
    13621 tgctgcagca tgatctcgct gagccagacc ttataagggt cttggcgctc ctttggcgga
    13681 gcacgccatg gcagcgccct cgcctcgcgg tcgtaccagg cgagcacggc cctttcgatc
    13741 gcgcgcggct ccagcgccat gtgactttcc tgcataagtc ggttttcccg ccgggctctt
    13801 gatgagccat ggtgtaagct caggccctgc cagggcaagc gcggagcgca ggctctccac
    13861 ggcgataaac ggcaaggcga tggcccgaca aacgcgaaaa cccagatatg gcgcgcgcgc
    13921 cattggctct tacgtggccc gggcgctcga tccggcggcg cgggcaaggg gcttcgccac
    13981 cacggcgctc ctcagcgact ggccggccat cgtgggccgc gatcttgccc gcttcaccat
    14041 gccggaccgg gtcatctggc ccaggcgccg cgatgaggag gcggagacgc cggcccgcgg
    14101 gcacaaggcc gagggcgcca ccctggtgct gcggatcgag ggcccgcgcg ccatcgaggt
    14161 ccagcaccgc tcggggcaga tcctggagcg ggtgaacggc tatttcggct atcgcgcggt
    14221 gaccgaaatg cgcttcctgc aagcgcccat cgcccgggtc cgcaaaaccc ctccggccaa
    14281 aagaccgccg ctgcctgcct attcgctgcc caaaacggtg gacatcgaag acgaaggtct
    14341 ggcccaggcg ctcacgaagc tcggcgccgc caccaaggcc cgcctcacga gggcttgagc
    14401 ccaaagacct ttctcgcact tgcccgatac gggccgatca acgattgtag gccccgccca
    14461 cgcgcccatc acctgggacc gccaagcacc ccaggagcgc gcccgcttgg gccgcaagaa
    14521 accagccatt tccggagtag taccgtggcc aaacccaaga agccgaccga gccctcaacc
    14581 gaagacacca aaatcaacaa gaccggactg attgtcggcg gcgcggtgct tgccgccgtg
    14641 gtggtcgccg gcatcgtcct cttcgacgac cgcggaggcg cgcccgaccc caacgcaccc
    14701 aagatggatc ccgaaatggc cgcgctgatg gaggcggggc cgctggagga tatcgtcctc
    14761 ggcaatgcag atgcgccctt cgtcatcgtc gaatacgcct cgatgacctg cccgcactgc
    14821 gcgaacttct atacggaggt gttcccgcag gtgaaggaga agtacatcga cacgggcaag
    14881 gcccgcttca tcttccgcga gttcccgcta gacgggcttg ccgtcgccgc ctccatgctg
    14941 gtgcgctgct ccggcaacga ccgcttctat ccgatgctcg acggcctgtt cgaaacccag
    15001 gagacctggg cggcgcccgg cgaggacggc aaggaaaagc tgctgatgat cgccaagcag
    15061 gccgggttct cgcaggaagc cttcgacaag tgcctggccg acaaggagct tttcgacaag
    15121 atcgtcgccg tgcgcaagaa ggcgcacgaa gaatatggcg tcgacgccac gccgagcttc
    15181 ttcgtcaacg gcaagcggct gaagggcatc gggatcgata agttcgaggc cgcgatcgag
    15241 gggaccggca agccggatac cccgcccagc ggctagactc acggtgatcg atgggacggg
    15301 ttagaggagc cgcgctggtt gtcatcgccg cgctcggcct ttcggggtgc ggcggcggcg
    15361 cgggcacgcc tacggaagcg gccgtccccc tcaaagaaga gatggtgcag ccgggaccgc
    15421 tcggggaccg cgtgtacggc aacaagaatg cgcccgtgac tatcatcgaa tatgtgtccc
    15481 tcacctgccc gtattgccgg caataccacg ccaaggtgtt cccgaaggtg aagcggcaat
    15541 acatcgatac gggcaaggtg cgctatatca tccgcgagtt ccccatcggc cggactgcgg
    15601 gcaacgcggc gatcgtcacg gcttgcgtgt ccaaaagcaa atatttgccc ttggtagagg
    15661 ctttcctctc ccggcagcgc gaatgggtca gccaggaggt gcgccccaac gccatctatt
    15721 cagtggccaa atccagcggt atgagccgcg cagaatttga caaatgccta tcgaatcaat
    15781 ccattatcga tggcctgacg gtggtgaagg agcgggggcg caaatacggc gtgatcggca
    15841 cgccgagctt cttcatcaat ggccaaaaac tccaaggcac cgtcacgttc gaggagatca
    15901 aggcgctgat tcagccacag gcatcttgac cgctgccatt gcgaatcacg cccaaccgct
    15961 gccaagttgc ccggtaacag tacgcgttct tccacgcgtc gctcctagtc catttaagga
    16021 tcgctagccc gtgcagatca ccagactccg cctactcgga ttcaagtcgt tcgtggagcc
    16081 gaccgagctc ctggtcgaga ccggcctcac cggggtcgtc ggcccgaacg gctgcggcaa
    16141 gtccaacctg ctcgaggcgc tccgctgggc catgggcgag acctcttata agagcatgcg
    16201 cggctcggca atggacgatg tgatcttcgc cggctcccag gaccggcccg cgcgcaacac
    16261 cgccgaggtg acgctgttcc tcgacaacag caagcggctg gcgcctgccg agttcaacga
    16321 ctcggacttg cttgaggtga cgcggcgcat cgagcgcgag gcggggtccg cttacaagat
    16381 caacggcaag gacgtgcgcg cccgggatat ccggctgcta ttcgaggacg ccgcgacagg
    16441 cgcccgctcg ccggcactgg tgcagcaggg ccgcatcggc gagatcgtca atgccgcgcc
    16501 gcaagaacgc cgccgcgtgc tggaagacgc cgccggcatt gccggcctgc acagccgccg
    16561 ccacgaagca gaattgcgcc tcaaggcggc cgagaccaac ctcgggcggc tcgccgacct
    16621 catcggccag atcaagtcgc aactcaacgc catgcgccgc caggcgcgcc aagccaaacg
    16681 ctaccggcaa atctccgaca agatccacga gatggaggcc ttgcagtacc acttgcaatg
    16741 gacggccgct tgcgggcagg tcgaagccga cgaggcggcc ttccaagagt cgctgtccgc
    16801 ggtcgccgaa gagaccaagg cggaaggcga ggccttaagg cgccaagccg aggcggccga
    16861 gaagcttcag cctctgcgcg atgcggaagc cgagcgcgcg gcggtgttgc accgtttgag
    16921 cgtggaacgg gacgcgctgg agcgcgaaga ggcccgcgcc aaggctcgcc aggcggaatt
    16981 ggagacgcgg ctggcgcaag cctcccgcga cctcgcccgc gaggaagaac acatcgccga
    17041 ggccgaaagc atgttgcgcg gactgggtga ggaaagccgc aagctcgtag cgtcgagcga
    17101 acgcgacgag gaggagccgc agctccgcgc cgccgccgcc aaggctgtcg aggcgcgtat
    17161 cgccgcggag gatgcgctgc gccaggccac caccgagctc gctgaggcaa aagccgaacg
    17221 cggccgcttg gaaggcgaga agaccacgac cgccgcgcgg atcgcgcagc tcgaatccca
    17281 agcgggcgac gtcgaacagg aactcgccaa gctggaagcg gagaccggcg gcagcgccca
    17341 attcgccgaa ctgcgcgcgg acgccgaccg gctcaccgct gccttgcagg agactgagac
    17401 agcactcgcc aaagccgagg cggacgagga gcaggcccgc gccgaggaag cacgcttacg
    17461 ttcggacgca gccgcggcca agctctcccg ccaggagatc gagaccgagt tcgccacgct
    17521 gaccaagctg ctcgcgccgg cccacgattg gacgccgatc gtcgaagagg tgagtgtcga
    17581 gggcggatac gagcaagctc ttggcgccgc gctcggcgat ga
//
LOCUS       DBNHVU010000135         4519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_348660, whole genome shotgun sequence.
ACCESSION   DBNHVU010000135 DBNHVU010000000
VERSION     DBNHVU010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_348660"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="AC16Y7_05405"
     CDS             complement(<1..479)
                     /locus_tag="AC16Y7_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5708849.1"
                     /translation="MNETARNLSKDSVEVGANVPLPVAVRDLTKRYGTVMAVNHISFT
                     VEPGATVALLGGNGAGKTTTIAMLLGLIKPTAGEVRVFGADMARDRYSVAQRMNFQSP
                     YVDLPARLTVKQNLTVYAELYGIANAGERIGYVAEHLQIEDLLGRQTGKLSAGQKTRV
                     "
     gene            544..1947
                     /locus_tag="AC16Y7_05410"
     CDS             544..1947
                     /locus_tag="AC16Y7_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActS/PrrB/RegB family redox-sensitive histidine
                     kinase"
                     /protein_id="HIR5708850.1"
                     /translation="MAALDNSQTADAGGGPEWLRAVDSRLRLHTIVRLRWLAVLGQSL
                     TVIFAYWVLGLNVPIGWCFAVIAASAWLNVALRIRYPSSHRLKSNHASLMLGYDVLQL
                     AALLYLTGGLANPFAFLLIAPVTVSASTLPIRITVALGILTIIAASVLTKYHYPLPWF
                     GGESLALPLMYVFGVWAAVVAGILFIGLYSWRTAEEGRRMAEALAAAEMVLAREQKLS
                     ALDGLAAAAAHGLGTPLATIAVVTKELLHDTKGDDPHFDDLTLLRAQAERCRQILKEL
                     AEHGEQLDIIVSRLPVSHLIDEVVEPHRLMAVPIEITARPVPGTEPGAPAALEPVTQR
                     NPGVIYGLGNLVENAVDFAATKVDVEADWSTDEVRIVIADDGEGFPPNVLEQLGEPFV
                     TTRPGKSWGDEEPDEHIGMGLGFFIAKTLLERSGARLELANCPEPQTGAVVTVVWPRA
                     KFERAASIEPDTPAPIL"
     gene            2156..2731
                     /locus_tag="AC16Y7_05415"
     CDS             2156..2731
                     /locus_tag="AC16Y7_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActR/PrrA/RegA family redox response regulator
                     transcription factor"
                     /protein_id="HIR5708851.1"
                     /translation="MEAQVSTFSPQGMLMPPEDNTLLIVDDDKAFLQRLARAMEARGF
                     DVEAAASVEEGLASLKRRPPAFAVVDMRLDDGNGIDVIATLKEVRPESRAVVLTGYGN
                     IATAVSAVKIGAVDYLAKPADADDVYNALTASPNDKAPPPENPMSADRVRWEHIQRVY
                     ELCNRNVSETARRLNMHRRTLQRILAKRAPK"
     gene            complement(2744..3223)
                     /locus_tag="AC16Y7_05420"
     CDS             complement(2744..3223)
                     /locus_tag="AC16Y7_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HIR5708852.1"
                     /translation="MLQSDQDPLISVDGRQSEAAAAIQRGVCRTFRALGHAVVTELPL
                     ANGRRADVVGLSPSGDVLIVEIKSCLVDYRTDGKWHEYLDYCDRLYFGVASDFPCEVI
                     PEAAGLILADRYGAELVREPAEERLNAARRKAMMLNFAHAAALRLQHQLDPGCGFKS"
     gene            3351..>4519
                     /gene="egtB"
                     /locus_tag="AC16Y7_05425"
     CDS             3351..>4519
                     /gene="egtB"
                     /locus_tag="AC16Y7_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HIR5708853.1"
                     /translation="MSLSHAVLAEPRREAPSQATSLRDRLMQTRRLSLRLAGPLSDED
                     QVVQAMDDASPTKWHLAHTTWFFEAFVLPRFSPGYRLFDEAFEYCFNSYYEAVGPRHP
                     RPKRGLLTRPSAQAVRDYRAHVDAGLERLLETGLPAEAEALIELGVNHEQQHQELLLT
                     DILSLFAAEPLKPAYREGAPRLSAGEPAPLAFSAFDGGVHTVGHDGDGFAYDNEGPRH
                     EQLIRPFKLANRCVTNAEWIQFIEDGGYRTHTLWLADGWNTINAEGWRGPLYFEEADG
                     GYMQMSLLGFRPVEPAAPVTHVSYFEADAFARWAGCRLPSEFEWEHASLSLPVTGRDL
                     GADHLRPLPAANETGLTQMFGDVWEWTSSAYLPYPGFKAAPGAVGEYNGKFMCNQ"
BASE COUNT          814 a         1478 c         1383 g          844 t
ORIGIN      
        1 acgcgcgtct tctgtccggc agagagcttt ccggtctgcc ggccgagtag atcctcgatc
       61 tgcagatgct cagcgacata gccgatccgc tcgcctgcgt tggcgatccc gtagagttcg
      121 gcatagaccg tgaggttctg cttcacggtc agccgggccg gaagatccac ataaggactt
      181 tggaagttca tgcgctgcgc cacgctgtag cggtcgcggg ccatgtccgc gccgaacaca
      241 cgcacctcgc ccgccgtcgg cttgatgagc cccagcaaca tggcgatggt tgtggtcttg
      301 ccggcgccgt tgccgccgag cagtgcgaca gtggccccgg gctcgacggt aaaactaatg
      361 tgattgacgg ccataaccgt gccatagcgt ttggtgagat cgcgtacggc gacggggagg
      421 ggcacgttcg cgcccacctc aacactatct ttcgagagat ttcgggccgt ctcgttcatt
      481 tctttgcact gcgcgcctgc tttgtcggaa ttatgatttc ccgccacatg ctaaggttca
      541 cctatggctg cactcgacaa tagtcaaacc gccgacgctg gcggcggacc ggaatggttg
      601 cgcgctgtcg acagccggtt gcgcttgcat acgatcgtgc gtctgcgctg gctcgccgtg
      661 ctcggtcaga gcctgacggt gatctttgcc tactgggtgc tcggcctcaa cgtgccgatc
      721 ggttggtgct tcgcggtcat cgcagcgtcg gcctggctca atgtggcgtt acgcatccgc
      781 tacccgtcga gccaccggct gaaaagcaac cacgcctccc tcatgctggg ctatgacgtt
      841 ctccagcttg ccgcgcttct gtatctcacg ggcggcctgg cgaacccctt tgccttcctg
      901 ctcatcgcac ccgtcacggt atccgcttcg accttgccga tacggatcac ggtggcgctg
      961 ggcatcctca ccattatcgc ggcgagcgta ctgaccaagt accactaccc gcttccctgg
     1021 ttcggcggcg agagccttgc ccttccgctc atgtacgtct ttggggtctg ggcggcagtg
     1081 gtcgccggca ttctgtttat cgggctctac tcctggcgca cggccgaaga gggccggcgc
     1141 atggccgagg ccttggcagc cgccgagatg gtgctcgcgc gtgaacagaa gctttccgcg
     1201 ctggacggct tggctgcggc ggcggcgcat gggctcggca cgcccctggc cacgattgcc
     1261 gtggtgacca aggagctcct gcacgacaca aaaggggacg atcctcactt cgacgacctg
     1321 accttgctcc gtgcgcaagc cgagcgctgc cgccagatct tgaaggaact tgccgagcac
     1381 ggcgagcaac tcgacatcat cgtctcgcgc ttgcccgtca gccacttgat cgacgaggtg
     1441 gtcgagccgc acaggctcat ggcggtcccc atcgagatta cggccagacc ggtgcccggc
     1501 accgagccgg gcgcacctgc ggcgctcgag cctgtcacgc aacgcaatcc cggcgtcatc
     1561 tacggtctcg gcaatctcgt ggagaatgcg gtcgacttcg ccgcgaccaa ggtcgatgtc
     1621 gaggcggatt ggtcgacgga cgaggtgcgc atcgttatcg ccgacgacgg cgagggcttt
     1681 ccgcccaacg ttctcgagca gcttggcgag cccttcgtca cgacgcggcc cggtaagagc
     1741 tggggagacg aagagccgga cgagcatatt ggcatgggcc tcggcttctt catcgccaag
     1801 acgttgctcg agcggtcggg cgcacgccta gagcttgcca actgtccaga accgcagacc
     1861 ggcgccgtgg tgaccgtggt ctggccccgc gcgaagttcg agcgagccgc gagcatagag
     1921 ccggacacac ccgcgccgat cctgtgattt tcggctttcg taccgttctt gatgcgcggc
     1981 agccccaaat gggcctgatt tcaactgatt ccgttcactt tgacggtgcg ttgcgcaggc
     2041 cgaaggcttg ccaccccaca cgaaagcgcc tacctgtacc tcaaaagaaa ttcactaatg
     2101 caattgggag cttacgccag ggttgggctt cgagaacgta acgaggagct catcaatgga
     2161 agcccaggtc tccacctttt cgccacaggg catgctcatg ccaccggaag acaatacgct
     2221 tttgatcgtc gacgatgaca aggccttcct ccagcgcctt gcacgcgcca tggaagcacg
     2281 cggctttgac gtcgaagcag cggcgtccgt cgaagaaggc ttggcctcgc tcaagcggcg
     2341 cccaccggca ttcgccgtgg tggacatgcg actcgacgat ggcaacggca tcgacgtgat
     2401 tgccacgctg aaggaagttc gtcccgagtc ccgcgccgtc gtgctgaccg gctatggcaa
     2461 catcgcgact gccgtgagcg cggtgaagat cggcgcggtc gactatctgg cgaagccggc
     2521 ggacgccgac gacgtctata acgcgctaac cgcttcgccc aacgacaagg cgccgccgcc
     2581 ggagaatcct atgtcggcgg accgcgtgcg ttgggaacac attcagcggg tctacgagct
     2641 gtgcaaccgc aacgtctcgg agacggcgcg gcggctcaac atgcaccgcc gaacgcttca
     2701 gcgtattctc gccaagcgcg cgccgaaata attttcagcg ccttcagctt ttgaaaccgc
     2761 agccgggatc gagttggtgc tggaggcgca aggctgcagc gtgcgcgaag ttcagcatca
     2821 tggccttacg gcgcgcggcg ttgagccgtt cctcggcggg ctcgcgcacg agctccgcac
     2881 cgtagcgatc ggccaagatc agaccggctg cctcgggaat gacctcgcac ggaaaatccg
     2941 acgcaacgcc gaagtaaagc cggtcgcaat aatccagata ttcgtgccac ttcccgtccg
     3001 tgcggtaatc gaccaggcac gatttgatct cgacgatcaa cacgtctccc gacggcgaca
     3061 ggccgacgac gtcggcgcgg cgcccgttcg ccagcggcaa ctcggtgacc acggcgtgac
     3121 ccagtgcacg aaacgtgcgg cagacgccac gctggatcgc ggccgccgcc tctgactggc
     3181 gcccgtccac ggaaatcaac ggatcttggt cggactggag cactggacag cgtccccgga
     3241 aaatcaaaca atcgtcacgg aattgtgcat ggttgcgcgt ttcctgaata ggaacaagct
     3301 gctaaatcgc acgttgcgag tcagaccccc gaacccccac gggaggaacc atgagcctca
     3361 gtcacgccgt cctcgccgag ccccggcgcg aagccccttc gcaagcaaca tccctccgcg
     3421 atcgcctgat gcagacacgc cgcctgtcac ttcggctcgc gggcccattg agcgacgagg
     3481 atcaagtcgt ccaggccatg gacgatgcaa gccccaccaa atggcacctt gcgcatacca
     3541 cgtggttttt cgaggcgttc gtgctgccgc gcttttcccc cggctaccgc ctattcgatg
     3601 aggcgttcga atattgcttc aactcctatt acgaggccgt cggaccgcgg catccgcgcc
     3661 cgaagcgcgg gttgttgacc cggccttcgg cgcaggccgt gcgcgattac cgcgcccatg
     3721 tggatgcagg ccttgagcgc ttactcgaga ccggcctgcc ggctgaagct gaggcgctca
     3781 tcgaactcgg cgtcaaccac gaacagcagc atcaagagtt gctgctgacc gacatcttga
     3841 gcctgttcgc cgccgaaccg ctcaagcccg cttatcgcga aggcgcgccc cgcctttctg
     3901 caggcgagcc cgcaccgctc gcgttcagcg cctttgacgg cggcgtgcac actgtcgggc
     3961 acgacggtga cggcttcgcc tatgacaacg aaggcccgcg ccacgagcag ttgatccggc
     4021 ccttcaagct cgccaatcgc tgcgtcacca atgcggagtg gattcaattc atcgaggacg
     4081 gcggctatcg cacgcacacg ctgtggctcg ccgacggctg gaatacgatc aatgccgaag
     4141 gctggcgtgg accgctctat ttcgaggagg ccgatggcgg ctacatgcag atgagccttt
     4201 taggtttccg ccccgtcgaa ccggccgcgc ccgtcaccca tgtgagctat ttcgaggcgg
     4261 atgcgttcgc gcgttgggcc ggttgccggc tgccgtcgga gttcgaatgg gagcacgcct
     4321 cgttgagcct gcccgtgacg ggccgcgacc tcggcgccga ccacttgcgg cctttgccgg
     4381 ccgcgaacga gacggggcta acccagatgt tcggcgatgt gtgggaatgg acgtcaagcg
     4441 cctacctgcc ttacccgggc ttcaaggctg cccccggcgc cgtcggtgaa tacaacggca
     4501 agttcatgtg caaccagtt
//
LOCUS       DBNHVU010000136         4631 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1463587, whole genome shotgun sequence.
ACCESSION   DBNHVU010000136 DBNHVU010000000
VERSION     DBNHVU010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1463587"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /gene="lysA"
                     /locus_tag="AC16Y7_05430"
     CDS             complement(<1..1009)
                     /gene="lysA"
                     /locus_tag="AC16Y7_05430"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR5708854.1"
                     /translation="MHHFAYRRGVSEANVLHAEEVDLCDLASSVGTPFYCYSTATLER
                     HYRVFAEAFAARPDTLLCYSIKANSNLGVLSTLAKLGAGMDVVSEGELRRALKVGVPG
                     DRIAFSGVGKTQAEMAHALEAGIYAFNVESEPELHALNEVAEAKGTTARIAFRVNPDV
                     DAGTHHKISTGKAEDKFGVPFEQALSLYQKAVALPGVVPAGVHMHIGSQITELAPFQN
                     AFALLTELVSTLRGAGLTIDFVNLGGGLGIPYRSDAPAPPLPTDYARLVNEALGHLDA
                     RLLFEPGRMIAGNAGILVARVLYVKTSPHKNFTIVDAAMNDLIRPTLYDAYHQVLPVT
                     EA"
     gene            complement(1019..1207)
                     /gene="lptM"
                     /locus_tag="AC16Y7_05435"
                     /gene_synonym="yifL"
     CDS             complement(1019..1207)
                     /gene="lptM"
                     /locus_tag="AC16Y7_05435"
                     /gene_synonym="yifL"
                     /inference="COORDINATES: protein motif:HMM:NF047847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS translocon maturation chaperone LptM"
                     /protein_id="HIR5708855.1"
                     /translation="MGSALRILLALMLISAVGLSACGRRGSLERPQSAYPQTDADPSA
                     KMSRAPDRRLPILDALIE"
     gene            complement(1279..2676)
                     /gene="argH"
                     /locus_tag="AC16Y7_05440"
     CDS             complement(1279..2676)
                     /gene="argH"
                     /locus_tag="AC16Y7_05440"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR5708856.1"
                     /translation="MTNTMWGGRFAASPSEIMEEINASIDFDKRLWRHDIEASKAHVA
                     MLAATGIVSEPEADEIAKGLDRILAEMEAGDFKFARELEDIHLNVEHRLTELIGPVAG
                     KLHTARSRNDQVATDFKLYVRDEIVTIEAGLAALQLALATTAIETADAVMPGFTHLQT
                     AQPVTFGHHCLAYVEMFGRDRGRFADAATRLNECPLGSAALAGTSFPIDRKMTAQALG
                     FTRPTANSLDAVSDRDFALETLAAASIAAMHLSRLADEIVLWTSPQFGFAKLSDAFST
                     GSSIMPQKRNPDAAELVRAKTGRIAGAFQALLLVMKGLPLAYAKDMQEDKEPVFDALD
                     ALRLGLAAMAGMVGDLTIDAKAMKKAAGREYATATDLADWLVQALDMPFREAHHATGK
                     IVALAEAKGADLKKLTLPDLRSVEPRITEDVYTVLDPARSAKSRKSQGGTAPTNVRRE
                     ARAWIKRLEKGGLSR"
     gene            2718..3377
                     /gene="tlpA"
                     /locus_tag="AC16Y7_05445"
     CDS             2718..3377
                     /gene="tlpA"
                     /locus_tag="AC16Y7_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020487305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein TlpA"
                     /protein_id="HIR5708857.1"
                     /translation="MDASNKRKHAGLPAKYVIAACLAVIAGFATVYLSLAPSDNGRTS
                     ASVKDEAAKGAGSGPLAGLNKGPMAAFVARKAPLDLPDVAFEGPDGAPMSLADLKGKV
                     VLLNIWATWCVPCREEMPQLNALQAGLGGGDFEVVAINIDKGGVDKAKAFLEETGATD
                     LAFYADPSAKLFAKLKAVGMPTTLLLNAQGQEIGRLVGPADWASPEAKKLIETAIKNA
                     G"
     gene            complement(3382..3801)
                     /locus_tag="AC16Y7_05450"
     CDS             complement(3382..3801)
                     /locus_tag="AC16Y7_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708858.1"
                     /translation="MLVPAHAEDTPEDIAEIIAEAAQVCRKAGGNPVTAPILRGDDLN
                     GDGHNDWIADFAKFKCTGAENPTCNPHGCTLQIYLGYGDGWELVFEDFVKAYKFSLSG
                     DTRTMHVTTSGIPCNKEASQTCQYNYRIEKDAVRPVQ"
     gene            complement(3870..>4631)
                     /locus_tag="AC16Y7_05455"
     CDS             complement(3870..>4631)
                     /locus_tag="AC16Y7_05455"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR5708859.1"
                     /translation="TKLGLAVGKSITLETAIRVIIVKSANDVSVMIAETVGGSEEAFI
                     DRMNAAAKKLGMTKTQFANPHGLPNEQQYTTARDLARLARALIIEFPEHSDIFAMKSV
                     QVGQKQLRTHNGLLRTFNGADGMKTGFICDSGFNIVVSATRQGRKLVAVVLGEPSTRV
                     RNARAARLLENGFKRYFWKSLFGTSLDGLAIQAKLDEQPAHLRQVICGPRPKKRRKRR
                     SVRRSSVSPELIGLHYTPVAQASGIPTAATLTAIN"
BASE COUNT          809 a         1422 c         1538 g          862 t
ORIGIN      
        1 cggcctctgt gaccggcagg acctggtgat acgcatcgta cagcgtcggg cggatgagat
       61 cgttcatcgc cgcatcgacg atggtgaaat tcttatgcgg cgatgtcttc acataaagga
      121 cgcgggcgac gaggatgccg gcattgccgg cgatcatgcg gcccggctcg aacagcaggc
      181 gcgcatcgag gtgcccaagc gcttcgttta ccagccgagc gtagtccgtc ggcagaggcg
      241 gtgcgggcgc atcgctgcga tagggaatgc cgaggccgcc gcccaagttc acaaagtcga
      301 tggtgagccc tgcgccacgc agcgtgctga caagttccgt cagcagcgcg aacgcgttct
      361 ggaacggcgc cagctcggtg atctggctgc cgatatgcat gtgcacgccg gccggcacga
      421 cgcccggcag cgcgacggct ttttggtaca gcgacagcgc ctgctcgaac ggcacgccga
      481 acttgtcctc cgccttgccg gtggagatct tgtgatgggt cccagcgtcg acatccgggt
      541 tgacccggaa ggcgatgcgg gccgtcgtgc ccttcgcctc ggccacttcg ttcagcgcgt
      601 gaagctccgg ctcggactcc acgttaaagg cataaatccc agcctccagc gcatgcgcca
      661 tttctgcttg cgtcttgccg acgcccgaga aggcgatgcg atcccccggc acgcccacct
      721 tgagcgcccg gcgcagctcg ccttcggaga cgacgtccat gcccgcgccg agcttggcca
      781 gcgtcgacag cacgccgagg ttggagttgg ccttgatcga atagcagagc agcgtgtcgg
      841 ggcgcgcggc aaaggcctcg gcaaacacgc ggtagtgccg ctcaagcgtc gcggtggaat
      901 agcagtagaa cggggtcccg acagaagagg ccagatcgca tagatcgacc tcctcggcgt
      961 gcagcacatt ggcctcagac acgccacgcc ggtaggcgaa atggtgcatg gggaaattct
     1021 attcgatcag ggcgtcgagg atcggcaagc gccggtccgg ggcacgagac attttggcag
     1081 acgggtcggc atcggtctgg ggataggccg actgcggccg ctcaagcgag ccacggcgcc
     1141 cgcaggcgga aagcccgaca gccgatatca gcataagcgc taacaggatg cgcaatgcgc
     1201 ttcccatggg tttgatccct gtggtgtcgt gccccttaag aatcagggcc tcttgccggg
     1261 cctctttgcc cctctctttt atcgcgagag gccgcccttc tccaaacgct ttatccacgc
     1321 tctcgcctcc cgccgaacat tagtgggggc cgttccgcct tggcttttgc ggctttttgc
     1381 cgaccgggcg gggtccagga ccgtatagac gtcctcggtg attctgggct cgaccgaccg
     1441 gagatccggc agggtcagct tcttcagatc ggcgcccttg gcctcggcga gggccacaat
     1501 cttgcccgtt gcgtggtggg cctcccgaaa gggcatatcc agagcttgca ccagccaatc
     1561 tgcaaggtcg gtggcggtgg cgtattcgcg gcccgccgcc ttcttcatcg ccttggcgtc
     1621 gatcgtcaga tcgcccacca tgccggccat ggcggcgagg ccgagcctca gcgcatccag
     1681 cgcatcgaac accggctcct tgtcctcctg catgtccttg gcgtaggcca gcggcagccc
     1741 cttcatgacc aggagcagcg cttggaaggc gccggcaatg cgcccggtct tggcgcgcac
     1801 cagctcggcg gcgtccgggt tgcgcttttg cggcatgatg gacgagccgg tggagaaggc
     1861 atccgacaac ttggcgaagc cgaattgcgg cgaggtccac agcacgatct cgtccgccag
     1921 cctcgacaga tgcatggccg cgatcgaagc tgcggcaagc gtctccagcg cgaaatcccg
     1981 gtcggacacc gcgtccagcg agttcgccgt cggccgcgta aagccgagcg cctgcgccgt
     2041 cattttgcgg tcgatgggaa aactcgtacc ggccagcgcc gcggagccaa gcgggcattc
     2101 gttgagccgc gtggcggcat cggcgaaccg tccccggtcg cggccgaaca tctccacata
     2161 ggccaggcaa tgatggccga aggtgaccgg ctgcgcggtc tgcaaatgcg tgaagccggg
     2221 cattaccgca tcggcggtct ctattgccgt ggtcgcgagc gcgagctgaa gcgcggcgag
     2281 acccgcctcg atggtgacga tctcgtcccg cacatagagc ttgaagtcgg tggccacctg
     2341 gtcgttgcgc gagcgggcgg tgtgcagctt gcctgccacg gggccgatca gctcggtgag
     2401 ccggtgctcc acgttgaggt ggatatcctc aagttctcgc gcaaatttga aatcgccggc
     2461 ctccatctcg gctaggatcc ggtcgagacc ttttgcgatc tcgtccgcct ccggctctga
     2521 gacgataccg gtcgccgcaa gcatggcgac gtgggccttg gaggcctcga tgtcgtgccg
     2581 ccacagccgc ttgtcgaaat cgatggaggc atttatctcc tccatgatct ccgacgggct
     2641 tgcggcaaaa cggccgcccc acatggtgtt ggtcatgatc gtccaggcct tagcgggcaa
     2701 agttcaaaga ggatgacgtg gacgcttcta ataaacgtaa gcacgccggc ttaccggcca
     2761 agtacgtaat cgccgcctgc cttgcggtga ttgccgggtt cgccacggta tatttgagtc
     2821 tagccccgtc tgacaatggg cggacgtctg cttcggtaaa ggacgaggcc gccaaagggg
     2881 ccggaagcgg gccgcttgcg ggcctgaaca agggccccat ggccgctttc gtggcgcgga
     2941 aggcgccgct cgatcttccc gacgttgctt tcgaggggcc ggacggggcg ccgatgagcc
     3001 tcgccgacct caagggcaag gtcgtgctcc tcaacatctg ggcgacgtgg tgcgtgccct
     3061 gccgggagga gatgcctcag ctcaacgcgc ttcaggccgg gctcgggggc ggcgacttcg
     3121 aggtggtggc gatcaatatc gacaagggcg gtgtggacaa ggccaaggcc ttcctggagg
     3181 agaccggcgc cacggatctc gcgttctacg ccgatcccag cgccaaattg ttcgccaagc
     3241 tcaaagccgt cggcatgccg acgacgcttt tgctcaatgc ccaaggtcaa gagatcggcc
     3301 ggctggtggg gccggcggac tgggcctcgc cggaggcgaa gaagctcatc gaaacggcga
     3361 tcaagaacgc gggctaggcg tctactgcac cggccgcacc gcgtccttct cgatacggta
     3421 gttgtattgg cacgtctgcg aggcctcctt gttacacggg atgccggagg tggtgacatg
     3481 catggtgcgt gtgtcgccgc tcaaggagaa cttgtacgct ttaacgaagt cctcgaagac
     3541 gagttcccag ccgtcgccat atccgagata gatctgaagc gtgcagccgt gcggattgca
     3601 ggtggggttt tcagcgcccg tgcatttgaa cttggcgaag tcggcgatcc aatcgttgtg
     3661 cccgtcgccg tttagatcgt cgccgcgcag gatgggtgcg gtcacgggat tgccgcccgc
     3721 cttgcggcaa acttgcgcgg cctcggcgat aatctcggcg atgtcctcgg gcgtgtcttc
     3781 tgcgtgggcc gggacaagca tcgcggcgca cgcaagggac gccaaaagag cgaggcgaca
     3841 acgcaggacg aaagacaaca ggaggaggct cagttgatgg cggtcagggt ggccgcggtg
     3901 ggaataccgg aggcttgcgc gaccggcgtg tagtggagcc caatcagctc agggctcacg
     3961 ctcgatctgc ggacggaccg gcgtttgcgc ctcttcttcg gcctcggtcc gcaaatgact
     4021 tggcgcaagt gtgcaggctg ctcgtcgagt ttggcttgaa tggcgagccc atccaggctc
     4081 gtgccgaaca gcgatttcca gaaatagcgt ttgaagccgt tctccagaag ccgcgcggcg
     4141 cgggcgttac gtacccgcgt ggacggctcg cccagaacca cggccacgag cttgcgccct
     4201 tggcgggtcg cgctgacgac gatgttgaat ccggaatcgc aaatgaagcc agtcttcatg
     4261 ccgtctgcac cgttgaaggt gcgcagcagg ccgttatgcg tgcgcagctg cttctgaccg
     4321 acttgcacgg acttcatggc gaagatgtcg gagtgctcgg ggaattcgat gatcagcgcg
     4381 cgggcgaggc gggcgagatc gcgcgcggtc gtatattgct gctcgttggg aaggccgtgc
     4441 ggattggcga attgcgtctt ggtcatgccg agtttcttgg cggcggcgtt catgcggtcg
     4501 atgaatgctt cctcgctgcc gcccaccgtc tccgcgatca tgaccgatac gtcattggcg
     4561 gacttgacga tgatcacccg gatcgccgtc tccagtgtga tggacttacc gaccgcgagg
     4621 ccgagcttgg t
//
LOCUS       DBNHVU010000137         4564 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_571811, whole genome shotgun sequence.
ACCESSION   DBNHVU010000137 DBNHVU010000000
VERSION     DBNHVU010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_571811"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /gene="lpxK"
                     /locus_tag="AC16Y7_05460"
     CDS             <1..747
                     /gene="lpxK"
                     /locus_tag="AC16Y7_05460"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - lipid-A 4'-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HIR5708860.1"
                     /translation="TAKGVVRVAGHEAHAVGDEPLLLAAVAPTFVSADRIAGAEAIEE
                     TDADVIVMDDGFQNPALAKDLSLIVVDAASGLGNGRVMPAGPLRAPLDTQMPRTDALL
                     VIGDGKKADRLVAAFETAGKPVLRAKVTPNCDARWLAVLPVIGFAGIARPNKFFATLK
                     SNGARLIESHSFRDHHRFTEKNARALLKEADEKKAMLVTTEKDWARIPDEDEDSALTE
                     LKNRSRPFPILVTFEDDEQAKDLLSALASR"
     gene            complement(744..977)
                     /locus_tag="AC16Y7_05465"
     CDS             complement(744..977)
                     /locus_tag="AC16Y7_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
                     /protein_id="HIR5708861.1"
                     /translation="MNRFERNLGFKGEAKLKYLDGEVQVITPGDFVRCAVTGQPIALD
                     ALRYWSVELQEPYADAAASLQRYSETHAKRDAS"
     gene            complement(1043..2482)
                     /gene="xseA"
                     /locus_tag="AC16Y7_05470"
     CDS             complement(1043..2482)
                     /gene="xseA"
                     /locus_tag="AC16Y7_05470"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR5708862.1"
                     /translation="MARRSPQAPQTNLAEYTVSELAFALRKTVEDAYGHVRLRGEVSG
                     YRGPHASGHCYFRLKDESAAIEAVVWRGTFGRLKFKPEEGLEIVAKGKITTYPGGSKY
                     QIVIEELEPAGVGALMALLEQRKKQLAAEGLFDEDRKTALPFLPRVIGVVTSPTGAVI
                     RDILHRLADRFPSHVLVWPVRVQGETCAAEVAAAIHGFNADPPQVAQRPDLIIVARGG
                     GSLEDLWGFNEEIVVRAAAASSIPLISAVGHETDWTLIDLVADYRAPTPTAAAERAVP
                     VRSELLTSVRTFGVRSAGSLRRALETWRSRFAASARGLPRRDAILGLPRQRLDAASHK
                     LPRALLANARVHRSQMERAGSRLRPHLLANTARTGRDSLLRLHRVKGRAIHATLDGAR
                     RRLDGQAKLLTTLGYHNVLARGFALVRAEDGVMLRRAADVKPGAGLDIEFADGHVEAH
                     ADPRRASGKKSAPQSAPRRKPDGNQGNLL"
     gene            2588..3862
                     /gene="purD"
                     /locus_tag="AC16Y7_05475"
     CDS             2588..3862
                     /gene="purD"
                     /locus_tag="AC16Y7_05475"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR5708863.1"
                     /translation="MNVLLIGGGGREHSLAWKLAQSPLLETLYCAPGNAGIAETATVV
                     SLDVGDHAAVAAFCKESGVGLVVVGPEAPLVAGLADDLEAQGIAVFGSSKAASQLEGS
                     KGFTKDLCAECSIPTGAYGRFTDANAAKAYAAEQQLPIVIKADGLAAGKGVVIATTAP
                     ETDSAIDACFEGAFGTAGAEVVIEEYLTGEEASFFALVDGTTALPLATAQDHKRVGDG
                     DTGPNTGGMGAYSPAPVMTEALSTRVMDEIIRPTVAAMAARGMPFKGVLYAGLMIEDG
                     APKLIEYNVRFGDPEAQVLMMRLKSDLLPALAATAQGTLGDIALEWHDEPALCVVMAA
                     KGYPGSYQKGTEIARLEDAGADPDVEIFHAGTAREADSIVAAGGRVLGVTARGQTIAE
                     AQSKAYAAVDTIDWPDGFSRRDIGWRAVGRAD"
     gene            4024..4392
                     /locus_tag="AC16Y7_05480"
     CDS             4024..4392
                     /locus_tag="AC16Y7_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708864.1"
                     /translation="MTYDDLPISKKTASGAVGVAAILFYLWLLTWAGPVQAMGLVTAP
                     ADASPTLTKAKIYKRGRSPVYPYATRGPGPRGWSSYFGFVPYTKGNVENEAIQRNFYP
                     MNSWPHDPNAPIPQPYGLGN"
BASE COUNT          816 a         1443 c         1514 g          791 t
ORIGIN      
        1 acggcgaaag gcgtcgtgcg cgttgcgggc cacgaggcgc acgcggttgg cgatgagccg
       61 ctgctgttgg cggccgtggc accgaccttc gtctcagcgg accgcatcgc gggcgccgag
      121 gccatcgaag aaacggacgc agacgtaatc gtcatggacg acgggtttca gaacccggcg
      181 ctcgccaagg atctgtcatt gatcgtggtg gatgcggcct ccgggctcgg caatggacgc
      241 gtgatgccgg cggggccatt gcgcgcgccg ctcgatacgc aaatgccacg caccgatgcg
      301 ctgctggtga tcggcgacgg taagaaggcc gatcgtctgg tcgccgcgtt cgaaaccgca
      361 ggcaagccgg tgctgcgcgc caaagtcacc cccaattgcg acgcccgctg gctcgccgtg
      421 ttgccggtga tcggctttgc agggatcgcc cggcccaaca agttcttcgc cacgctcaag
      481 tcgaacggtg cgcggctaat cgaaagtcat agctttcgag accatcaccg ctttaccgag
      541 aagaatgctc gcgcgcttct taaagaggcc gacgagaaga aggcgatgct ggtgaccacg
      601 gagaaggatt gggcgcgcat ccccgacgaa gacgaagaca gcgcgctcac cgaactcaag
      661 aatcgctcac ggccgttccc gatcctcgtg acgttcgagg acgacgagca agcgaaagac
      721 ctgctcagcg cgctggcaag ccgttagctt gcgtctctct tggcgtgggt ttcgctgtag
      781 cgttggagcg aggcggccgc gtcggcataa ggctcctgga gttccacact ccaataacgc
      841 aaggcgtcga gcgcaatggg ctggcccgtg acggcgcagc gcacgaagtc gccgggcgtt
      901 atcacctgga cctcaccgtc gagatatttg agcttggctt cgcctttgaa gccgagattg
      961 cgttcgaagc ggttcatcaa gtcggtggca ctctctcgtt gtgtctcagc atagcgtcac
     1021 ggcaaacccg ttgccagcgg ggctaaagca aattcccctg atttccatcg ggcttgcggc
     1081 gcggtgccga ttggggcgcg ctcttcttgc cggaggcccg ccgtggatcg gcatgcgctt
     1141 caacatggcc atcggcgaac tcaatgtcca gtccggcacc cggcttcacg tccgcagcac
     1201 ggcgcagcat gacgccgtcc tcggcgcgca ccaacgcgaa gccccgggcc aaaacgttgt
     1261 gataaccgag cgtcgtgagc agctttgcct ggccatcgag acgccggcgg gcaccgtcca
     1321 aggtcgcatg aatggcgcgg cccttgacgc gatggaggcg cagaagcgag tcgcggcccg
     1381 tgcgcgccgt gttcgccaga agatgcggcc gtagccgtga gccggcgcgt tccatctgcg
     1441 agcgatggac ccgcgcgttg gccagcagcg cgcgcggcag tttgtggctc gcagcatcga
     1501 gacgctgacg cggcaggcca aggatcgcgt cacggcgcgg taggccccgg gcagacgccg
     1561 caaagcggct gcgccacgtc tccaatgcgc gccgcaagga gccggcgctg cgcacgccga
     1621 atgtccgcac gctcgtcaga agttcggagc gcacgggcac ggcacgctcg gctgcggctg
     1681 tcggcgtcgg cgcccggtaa tcggcaacga gatcgatcaa ggtccaatcg gtctcgtgtc
     1741 caaccgccga gatgagcggg atcgagctgg cggcggccgc gcgcaccacg atttcctcgt
     1801 tgaagcccca taggtcctca agacttcctc cgccgcgcgc gacgatgatg agatcgggac
     1861 gctgtgcgac ctgcggcgga tccgcgttga agccatgaat ggcggcggcc acttcggcgg
     1921 cgcaggtctc gccttgtacg cggaccggcc agaccagcac atgggagggg aagcggtcgg
     1981 ccagccgatg aagaatgtcg cggattaccg cgcccgtggg cgacgtcacc acgccgatga
     2041 cgcgcggcag gaagggcagc gccgttttgc gatcctcgtc gaacagcccc tcggcggcga
     2101 gctgcttctt gcgttgttcc agaagagcca tgagcgcacc gacgcccgcg ggctccagct
     2161 cctcgatgac gatctgatat tttgaaccgc cgggataggt ggtgatcttg cccttggcga
     2221 cgatctccag cccttcctcg ggcttaaact tcaagcggcc gaaggtgccg cgccagacca
     2281 ccgcctcaat cgccgcgctc tcgtccttca gcctgaaata gcaatgaccg gaggcgtggg
     2341 gcccgcgata gccggagact tcgccgcgca gccgcacgtg accgtaagcg tcctccaccg
     2401 tcttgcgcag cgcaaaggcg agctcggaga cggtgtactc ggcgagattg gtctgcgggg
     2461 cctgtggact acggcgggcc atttcgtcat ctctttcgcg tggcgcgccc tgtcgtgcta
     2521 agagcgcgcg aatatccttg agggcataca ggcccggacg gcagcgaaca agcggggtct
     2581 tccgacggtg aacgtgcttc tgattggcgg tgggggacgt gagcattcgc tggcttggaa
     2641 gctggcgcag agccctcttc tggagacgct ttattgcgcg cccggcaatg ccggcatcgc
     2701 cgaaaccgcg acagtggtct cgctcgacgt gggcgaccac gcggccgtgg cggcgttttg
     2761 caaggagagc ggcgtcggcc tggtggtggt ggggcccgag gcgccgctcg tcgccgggct
     2821 cgccgacgat ctcgaagcgc aaggcatcgc cgtcttcggc tcctccaaag ccgcaagcca
     2881 gctcgaaggg tcgaagggct ttaccaaaga cctttgcgcc gaatgcagta tcccaacggg
     2941 cgcctatggg cgcttcaccg acgcaaatgc cgcgaaggct tacgcggcgg agcaacagct
     3001 tcccatcgtc atcaaggcgg acgggctcgc ggcgggcaag ggcgtggtga ttgccacgac
     3061 ggcacctgag accgattcgg cgatcgacgc ctgcttcgag ggcgccttcg ggaccgctgg
     3121 cgcggaggtt gtgatcgagg agtatttgac aggcgaagag gcgagcttct tcgccctggt
     3181 cgacggcacg acggcgttgc cgctggccac ggcgcaggac cacaagcgcg tcggcgacgg
     3241 cgatacgggc cccaacactg gcggcatggg cgcctactct ccggcgccgg tcatgacgga
     3301 agcgctctca acccgcgtca tggacgagat catccggccc acggtcgcgg cgatggccgc
     3361 acgcggcatg cccttcaagg gtgtgctcta tgccgggctg atgattgagg acggtgcacc
     3421 caagctcatt gagtacaatg tgcgcttcgg cgatccggag gcgcaggtgc tgatgatgcg
     3481 cctcaagtcg gacctcctgc ctgcgctggc cgccacagca caaggcacgc tcggcgatat
     3541 cgcgcttgag tggcacgacg agccggcgct ttgcgtggtg atggcggcca agggttatcc
     3601 cggcagctac cagaaaggca ccgagatcgc gcgcttggaa gacgccggcg ccgatccgga
     3661 tgtcgagatc ttccacgccg gtacggcacg tgaggcggac agcattgtcg ccgcgggcgg
     3721 ccgcgtcctc ggcgtcaccg cgcgggggca gacgattgcg gaggcccagt caaaggccta
     3781 cgcggcggtc gatacgatcg attggccgga cggctttagc cgccgcgaca tcggctggcg
     3841 cgctgtcggg cgcgcggatt gagcgagatc ccgcgcaaaa gttagcgcca tgttcatgga
     3901 tgggctgcaa aatgagcgcg aaccatagtt ttgccgtgct ttcttgcggt attggcgttg
     3961 cgttttcacg atggcgtcac tatttgcaag tcatctggca acgccagcga aaaagagaat
     4021 cgcatgacct acgacgacct gcccatctcg aagaagaccg cttctggcgc cgtaggcgtt
     4081 gccgcgattc tgttctacct ctggctgctg acctgggcgg ggcccgtcca agccatgggg
     4141 ctcgtcaccg cgccggcaga cgcctcgccg accctcacta aggccaagat ctacaagcgc
     4201 ggacggtcac cagtctatcc ttacgccacc agggggccag gcccgcgcgg ctggagcagc
     4261 tatttcgggt tcgtgcccta caccaagggc aacgtcgaga acgaggccat ccagcggaac
     4321 ttctatccga tgaattcctg gccgcacgac cccaacgcgc cgatccccca gccctacggc
     4381 ctgggaaact aatcgctctc cgccaatcat cgaggcgctt tgtcttggca tgcagcaagc
     4441 tctgtcttgc tgtgtcattg tcgttgcgcg cacctaggcg catcgtgatg taacgtcgaa
     4501 ctttcgaaaa attttagtcg aagaccttcg ccagtattcg tgttctgcgt gctggaggtc
     4561 ggca
//
LOCUS       DBNHVU010000138         6597 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1464249, whole genome shotgun sequence.
ACCESSION   DBNHVU010000138 DBNHVU010000000
VERSION     DBNHVU010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1464249"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1829
                     /locus_tag="AC16Y7_05485"
     CDS             <1..1829
                     /locus_tag="AC16Y7_05485"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR5708865.1"
                     /translation="LVKFADRLHNMRTLKHMKPAKRKIIAEETMDIYAPLAGRMGMQW
                     LRDELEDHAFRWLNPEAYTAVVERLKILREGNQGLIDEIQDELTTKLKQARVKGVASG
                     REKKPYAIWSKMERKQISLEQLSDIYGFRIVVPKVVDCYRALAVVHTTWHVVPGRFKD
                     YISNPKRNDYQSIHTTVVGPRHQRVELQIRTEMMHEVAEYGVAAHGLYKDLNGSNGKS
                     KVLSQESSAYRWLRHLVGTLLEGDNPEEFLEHTKLELFQDQVFCFTPKGRLIALPRGA
                     NCIDFAYAVHTDVGNTCVGAKINGRPMPLATALRNGDEVQIITSKAQLPPVAWQSIAV
                     TGKARSAIRRASRDAARSQYGKLGREILDRAFQRNDKTFEEELLTKALKRLSQKTADD
                     VIAAVGRGELASADVLRAVFPEDVPPAPPKRRREIKRTDEGWFGLGKVMGLKFRWPGS
                     GGKARPPGAGGIPIRGLQGDLPVNFAEGGAVPGDRIVGILTPGQGIMIYPIHANALKA
                     FDEEPERWIDVTWDIDEDDPQRFPAKIAVTAMNEPGSLAQIAAVIGEGDGNIENIKLT
                     NRVPDFTELLIDLEVWDLPHLNEILAGLRAKDVVSSAARADG"
     gene            1948..2490
                     /locus_tag="AC16Y7_05490"
     CDS             1948..2490
                     /locus_tag="AC16Y7_05490"
                     /inference="COORDINATES: protein motif:HMM:NF021363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HIR5708866.1"
                     /translation="MLFRRREAESFFERLRVSLWPRRSWSRSTRYVVHRIRRLSATPH
                     AVALGFAAGVFVSCTPFLGFHLIMASLVAWIVGGSIVAAVLGTFIGNPITYPIFWFIT
                     YEVGSLMLGRGAGKPHIDLSKEIFQTDQLWPIVQPMTLGAVPIGLALGALSYFLVRPA
                     VNAYQHRRRSLRGREAMDAR"
     gene            2487..3275
                     /locus_tag="AC16Y7_05495"
     CDS             2487..3275
                     /locus_tag="AC16Y7_05495"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HIR5708867.1"
                     /translation="MTAPPIRLGINIDHVATLRNARGGVHPDPLRAAKLAVEAGADNI
                     TAHLREDRRHIRDDDIRRLMAELDCPLNFEMAATDEMRAIALKVKPHAACIVPERREE
                     RTTEGGLDAVAQFDALVPVVADLTDAGIQVSLFIAAEGRQIEAASRLGVPAIEIHTGA
                     WCDALADGRGTDAESEWQCIKEGAQLAHGLGLEVHAGHGLNYETAETISALPEIVELN
                     IGHFLVGGAVFEGLGETVRKMRQAMDKGRQAATQVRTIENVDLA"
     gene            3275..3685
                     /gene="acpS"
                     /locus_tag="AC16Y7_05500"
     CDS             3275..3685
                     /gene="acpS"
                     /locus_tag="AC16Y7_05500"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018065979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR5708868.1"
                     /translation="MIIGLGNDVIDIRRVEKTIERFGERFLDRVFTDIERRKSDGRTL
                     RAASYAKRFAAKEACSKALGTGFRHGVFWRDMGVVNLPSGRPTLELTGGAARVLRELI
                     PNGHEARIDLTITDDFPSAQAIVIISALKFPARD"
     gene            3780..4535
                     /gene="lepB"
                     /locus_tag="AC16Y7_05505"
     CDS             3780..4535
                     /gene="lepB"
                     /locus_tag="AC16Y7_05505"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR5708869.1"
                     /translation="MSVGAEKVEAKGSARETVRIIIHALILALIVRVFLFQPFNIPSG
                     SMIPTLLIGDYLFVSKYSYGYSRYSFPGGFDLFSGRILAGEPKPGDVVVFKLPRDNET
                     DYIKRVIGLPGDEIQMIHGVLHINGKAVEKVKVDDYVVTGPSGRQRNMARYKETLPNG
                     VSYPVLDLVSDGFGDNTEVYKVPEENFFMMGDNRDNSTDSRFLSEVGFVPFENLVGKA
                     QVIFFSIDEDASFWEVWRWPTDVRWNRMFEAVH"
     gene            4540..5253
                     /gene="rnc"
                     /locus_tag="AC16Y7_05510"
     CDS             4540..5253
                     /gene="rnc"
                     /locus_tag="AC16Y7_05510"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR5708870.1"
                     /translation="MAKRRTTDHSQLCARLGHDFASPALLELALTHSSARPGGKPNDD
                     NERLEFLGDRVLGLSVAEILTHRFTEASEGELARWFNHLVRTETCAEVAQSWQLGDLI
                     VMSSGEASSGGRRKKTILANACEAVLGALFADGGYEVARTVVDDNWTPFLGELEDAAS
                     DAKSVLQEWAQGRQLPLPSYVEIAREGPDHAPRFTSEVQVEGVEPERGEGTSKRQAEQ
                     AAAQAMLEREGVWGTSDDE"
     gene            5246..6163
                     /gene="era"
                     /locus_tag="AC16Y7_05515"
     CDS             5246..6163
                     /gene="era"
                     /locus_tag="AC16Y7_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR5708871.1"
                     /translation="MSEHNENRTTRCGFVALIGAPNSGKSTLTNALVGTKVSIVTHKA
                     QTTRGPVRGIMLQDDAQIILVDTPGIFQPKRRLDRAMAQAAWDRAGDADIVALVIDAK
                     RGLDDKLAPIVEHLPEIKRPTIAILNKIDLVKKPDLLRLTSDLLALKTFADVFMVSAL
                     TGDGVEDLKTYFAKTVPESPWLFPEDQLSDASLRQTAAEITREKLFLRLHEELPYALT
                     VETAQWKELKDGSVRIEQTIYVERENQRRIVLGAKGAMIKEIGQAARTEIAEVAGVPV
                     HLFLFVKVRERWGEDPERFREMGLDYPKG"
     gene            6178..>6597
                     /gene="recO"
                     /locus_tag="AC16Y7_05520"
     CDS             6178..>6597
                     /gene="recO"
                     /locus_tag="AC16Y7_05520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR5708872.1"
                     /translation="MDWSDEGIFLSGKPLGEANLIAEILTLEHGRHLGLVRGGRSRRI
                     RPTLQPGNLVGVTWRARLSEHLGGYNVELMEAHGARALDDARALAAIGTLGELAKLLP
                     ERDPHPELYATTLHVLRSFGEPDIWPALLVYWEFQLLQ"
BASE COUNT         1280 a         2010 c         2136 g         1171 t
ORIGIN      
        1 tgctggtgaa gttcgccgac cgtctgcaca acatgcggac gctgaagcac atgaagccgg
       61 cgaagcggaa aatcatcgcc gaggagacca tggatatcta tgcgccgctg gccggccgca
      121 tgggcatgca atggctgcgc gacgagttgg aggatcatgc cttccgttgg ctgaaccccg
      181 aagcctatac ggcggtggtc gagcgcttaa agatcttgcg cgaaggcaat cagggcttga
      241 tcgatgagat tcaggacgag ctcaccacca agctgaagca agccagggtc aagggcgtcg
      301 cctcgggccg cgagaaaaag ccctacgcca tctggagcaa gatggagcga aagcaaatct
      361 cactggagca gctttccgac atttacggat tccgcatcgt ggtgccgaag gtggtggact
      421 gctaccgggc gctggccgtc gtacacacga cctggcacgt ggtgccgggc cgcttcaagg
      481 attacatctc caatccgaaa aggaacgatt accagtcgat ccacacgacg gttgtcggtc
      541 cgcgccatca gcgcgtcgaa ctgcaaatcc gcacggagat gatgcacgag gttgccgagt
      601 acggtgtagc ggcccacggc ctctataagg atctcaacgg cagcaacggc aagtcaaagg
      661 tgctgtcgca agagtccagc gcctatcgtt ggctgcgtca tctcgtcggg acattgttgg
      721 aaggcgacaa cccggaagag ttcctcgagc acaccaagct cgaactgttt caggaccaag
      781 tgttctgctt cacgccgaaa ggtcggctca tcgcgctgcc gcgcggcgcc aactgcatcg
      841 acttcgccta cgccgtacac accgatgtcg gcaacacctg cgtcggcgcc aagatcaatg
      901 gccggcccat gccgcttgcc acggcgctgc gcaacggcga cgaggtgcag atcattacct
      961 caaaggctca actgcccccg gtcgcgtggc agagcatcgc ggtgacgggc aaggcccgct
     1021 cggctattcg acgggcctcc cgcgacgcag cgcggtcgca atacggcaag ctcggccggg
     1081 aaattctcga ccgcgccttt cagcggaacg acaagacatt cgaagaagag ctgctcacca
     1141 aggcactcaa gcgcctgtct cagaagacgg ccgacgacgt catcgccgcg gtggggcggg
     1201 gcgaactcgc gtccgccgat gtgctgcgcg ccgtctttcc tgaggacgtg ccgccggccc
     1261 cgcccaagcg ccggcgggag atcaagcgga ccgacgaagg gtggttcggc ctcggcaagg
     1321 tcatggggct gaagttccgc tggccgggat cgggcggcaa ggcgcggccc ccgggggccg
     1381 ggggcatccc gatccggggc ttgcaaggag accttccggt gaactttgcc gagggtggcg
     1441 cggtgcccgg cgaccgaatc gtcggtattc tcacgccggg gcagggcatc atgatctacc
     1501 cgatccacgc caacgcgtta aaggcgttcg acgaggagcc ggagcgttgg atcgacgtca
     1561 cctgggacat cgacgaggac gacccgcagc gattccccgc caagattgcc gtgacggcga
     1621 tgaacgagcc cgggtctctg gcgcagatcg ccgcggtcat cggcgaaggc gacggcaata
     1681 tcgagaacat caagctgacc aacagggtcc cagacttcac cgaattgctg atcgatctgg
     1741 aagtgtggga cctcccgcac ctcaacgaga tcctggccgg cctcagggcg aaggatgtgg
     1801 tgagcagcgc ggcccgggcg gacggttagg cccgccaaaa taccgcgtac ccctggcata
     1861 tagagaaacg gaaaccaaat atggtagggc tccaggcgtc gaaggaccgg ccgctcgaca
     1921 gcggacagtc ctaatacgag ggtcgttatg ctgttcaggc gccgcgaggc ggagagcttt
     1981 ttcgagcggt tgcgcgtgtc tttatggccg cgccgcagct ggagtcgctc gacccgctat
     2041 gtcgtgcatc gcatacgccg cctgagcgcc acgccccatg cggtcgccct cggcttcgcg
     2101 gcgggcgtct tcgtttcgtg tacgccattt cttgggttcc acttgattat ggcctcactc
     2161 gtggcctgga tcgtcggcgg cagtatcgtg gcggcagtac ttggtacgtt catcggcaat
     2221 cccattacct atccgatttt ctggttcatc acgtatgaag ttggcagtct gatgctggga
     2281 cgcggcgcgg ggaagccgca tatcgatttg tcgaaagaga tctttcagac cgatcagctt
     2341 tggcccatcg tccaaccgat gacgctcggt gcggtgccaa tcggcttagc gctcggcgcg
     2401 ctgagctatt ttctggtgcg tcctgcggta aacgcttatc agcatcgccg ccggagcctt
     2461 cgaggccgcg aagccatgga tgcaagatga cggcgccacc catcaggctc ggcatcaata
     2521 tcgatcacgt cgccacgttg cggaatgcgc ggggcggcgt gcatcccgat ccgttgcgcg
     2581 cggccaagct cgccgttgag gcgggggccg acaacattac ggcgcatttg cgcgaagacc
     2641 gccggcatat ccgcgacgac gatattcggc gtttgatggc cgaactcgac tgtccgctga
     2701 actttgagat ggcggcgacc gacgagatgc gggcgatcgc cctcaaggtg aagccccatg
     2761 cggcgtgcat cgtgcccgaa cggcgcgagg agcgaaccac cgaaggcggt cttgatgcgg
     2821 tcgcgcagtt cgacgcgctt gtgccggtcg tcgccgactt aaccgatgct gggattcagg
     2881 tctccctgtt cattgccgcc gaagggcgcc agatcgaagc cgcctcgcgc cttggcgtgc
     2941 cggccatcga gattcatacc ggcgcctggt gcgatgcact tgccgacggc cgcggcacgg
     3001 acgccgagtc cgagtggcag tgcatcaagg aaggcgctca gctggcgcac ggcctcgggc
     3061 tggaagtcca tgctggccac gggctcaact acgagacggc ggaaaccatt tccgccctac
     3121 cggagatcgt cgagctgaat atcggtcact ttcttgtcgg cggcgcggtg ttcgaagggc
     3181 tgggtgaaac tgtgcggaaa atgcgccaag caatggacaa agggcggcag gcggcaacgc
     3241 aggtgcgcac gatcgagaac gtggatttgg cgtgatgatt atcggccttg gcaacgacgt
     3301 tatcgatatc cggcgcgtcg agaagaccat cgagcggttc ggggagcgat tcctggaccg
     3361 ggtcttcacc gacattgagc gccggaagtc agatggaagg acgctgcgcg cggcgtccta
     3421 cgcaaagcgt ttcgcggcca aggaggcctg ctccaaagcc cttggcaccg gctttcgcca
     3481 tggggtcttc tggcgggata tgggcgtcgt aaatttgcct tcaggccggc ccactctgga
     3541 gctcacggga ggcgccgcgc gggtgcttcg cgaattgata ccgaacgggc atgaggcgcg
     3601 tatcgacctc accatcaccg acgatttccc aagcgcgcag gcgatcgtca tcatctcggc
     3661 cttgaagttc ccggcacgcg attgaggcgt ttgcttgaac cttgaggagg ggctataggg
     3721 gcccctgtcc tccgggcgat gggggctgaa cgggtgttga aatcaaggag agccgcctga
     3781 tgagcgtagg cgccgagaag gtggaagcaa aaggcagcgc gcgggaaacg gtccgcatca
     3841 tcattcatgc gctgatcctg gcgctgatcg tgcgcgtatt tctgtttcag ccgttcaata
     3901 tcccctctgg ctccatgatc ccgactcttc tgatcggcga ttatctcttc gtttcgaaat
     3961 acagctacgg ctatagccgc tactcattcc caggcggctt cgacctgttc tcggggcgca
     4021 ttctggccgg ggagccgaaa cccggcgacg tcgttgtctt caaactcccc cgcgacaacg
     4081 agacggatta catcaagcgc gtcatcggcc tgcccggcga tgagatccaa atgatccatg
     4141 gcgtgctgca tatcaacggc aaggccgtgg agaaggtgaa ggtcgacgac tatgtggtga
     4201 ccgggccgtc tggccgccaa cggaacatgg cgcgctacaa ggaaacactc cccaacggcg
     4261 tgagctatcc cgtgctcgac ctcgtcagtg acggtttcgg cgacaacacc gaggtctaca
     4321 aagtgcccga ggaaaatttc ttcatgatgg gcgacaaccg cgacaactct accgacagcc
     4381 ggttcctttc cgaggtcggt ttcgtgccgt ttgagaattt ggtgggcaag gcgcaagtca
     4441 tcttcttctc catcgatgag gatgcaagct tctgggaagt ctggcgctgg cccaccgacg
     4501 tgcgttggaa ccgaatgttt gaggcggtgc actaaagcct tggccaaacg gcggaccacc
     4561 gatcacagtc aactctgcgc ccggctcggg cacgatttcg ccagccccgc gctccttgag
     4621 cttgccttga cgcattccag cgcgcggcct ggcggcaagc cgaacgacga caacgaacgc
     4681 cttgagttcc tcggtgaccg ggtgctcgga ctttcggtgg cggagattct cactcaccgc
     4741 tttacggagg cgagcgaagg agagctggcc cgctggttca atcatctggt gcgcacggaa
     4801 acctgcgccg aggttgcgca aagctggcaa ctcggggacc tgatcgtcat gagcagcgga
     4861 gaggcgagct ccggcggccg ccgcaagaag accatcctcg ccaatgcttg cgaggccgtg
     4921 ctcggcgcgc tgttcgccga cggcggctac gaggtggcgc gcactgtcgt cgatgacaat
     4981 tggacgccct ttctcggcga gttggaggac gccgcgtcgg acgccaagtc ggtgcttcag
     5041 gaatgggcgc aagggcggca attaccgctg ccgtcctatg tggagatcgc gcgcgaagga
     5101 cccgatcatg cgccgcgatt cacctccgag gtgcaggtcg agggcgtcga gccggagcgc
     5161 ggcgagggca ccagcaagcg ccaggcggag caggcggcgg cgcaggccat gctcgaacgc
     5221 gaaggcgtat gggggacgtc cgacgatgag tgagcacaac gagaacagga cgacgcgatg
     5281 cgggttcgtg gcgctgatcg gcgcgccgaa cagcggcaaa tcgactctca ccaatgcctt
     5341 ggttggcacc aaggtttcca tcgtcaccca taaggcgcag acgacgcggg gccccgtgcg
     5401 cgggatcatg cttcaggacg atgcacaaat catccttgtc gacacgcccg gtatcttcca
     5461 gcccaaacgc aggctcgacc gcgccatggc gcaagcggcc tgggaccgcg ccggcgatgc
     5521 cgacatcgtt gcactcgtga tcgacgccaa acgcgggctc gacgacaagc tcgcacccat
     5581 cgtcgagcat ctgcccgaga tcaaacggcc caccatcgcg attctcaaca agatcgatct
     5641 cgtgaagaag cccgacctgt tgaggctgac gtcggatctt ctcgcgctga agaccttcgc
     5701 cgacgtgttt atggtgtccg cgctgaccgg cgacggcgtt gaggacctga agacctattt
     5761 cgccaagacg gtgcccgaaa gcccgtggct ctttcccgag gaccagttgt cggacgcctc
     5821 gttgcgccag accgccgcgg agatcacgcg cgagaagctg ttcctcaggc ttcacgaaga
     5881 gttgccttac gcgctcaccg tggagacagc gcagtggaag gaactcaaag acggcagtgt
     5941 ccgcatcgag cagacgatct atgtggagcg tgagaaccag cggcgcatcg tgctgggagc
     6001 caaaggcgcc atgatcaagg agatcggcca ggcggcgcgc accgagatcg ccgaggtggc
     6061 aggcgttccc gtacacctgt tcttgttcgt gaaggtccgc gagcgctggg gcgaagatcc
     6121 ggagcgcttc cgcgagatgg gcctggacta tccgaaggga taggccaagc tgggcccatg
     6181 gactggagcg acgaaggcat ttttttgagc ggcaagccgc tcggtgaggc gaacctgatc
     6241 gccgaaatct tgaccctgga acacggccgt catctggggc tcgtgcgcgg cggccgctcg
     6301 cggcgcatcc ggcccacgct tcagcccggc aatctggtgg gcgtaacctg gcgcgcgcgc
     6361 ctgtctgagc atctgggcgg ctacaacgtc gaactgatgg aggcccatgg cgcccgcgcg
     6421 ttggacgacg cccgcgcgct cgccgccatc ggcactctgg gggagctcgc aaagcttctg
     6481 ccggagcgcg atccgcaccc cgagctctat gccacgaccc tccacgtgct gcgctccttc
     6541 ggcgaaccgg acatctggcc ggcgctgctc gtctattggg agtttcagct gctgcaa
//
LOCUS       DBNHVU010000139         4896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1242085, whole genome shotgun sequence.
ACCESSION   DBNHVU010000139 DBNHVU010000000
VERSION     DBNHVU010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1242085"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="AC16Y7_05525"
     CDS             <1..1143
                     /locus_tag="AC16Y7_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004446645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR5708873.1"
                     /translation="SAHICTGDGNAMVLRAGLPLQDMEFVQFHPTGVYGAGVLITEGA
                     RGEGGFLRNSEGEKFMERYAPSAKDLASRDVVSRAITIEIREGRGHGPEKDYMHLHLD
                     HLDEDMLAERLPGITDIARIFGGVDVRNEPIPIIPTCHYNMGGIPTNMHGEVIAPRGD
                     DKDAVVPGLMALGEAACVSVHGANRLGSNSLIDLVVFGRAAALRCAEIVGKDTAIPEL
                     PKGAGDEAIARLDRFRYADGGTPTAELRLAMQKTMQRDCSVFRTKEVLAEGVENIKKV
                     WDDAEDIAVTDRSLIWNTDLIETLEFDNLISQAAVTVQGALAREESRGAHAREDFPKR
                     DDKDWMKHTLSYASYGTHTVTLDFRPVHTYTLSNEVSYIEPKERVY"
     gene            1146..1934
                     /locus_tag="AC16Y7_05530"
     CDS             1146..1934
                     /locus_tag="AC16Y7_05530"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018062706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HIR5708874.1"
                     /translation="MVQFTLPKSSKIKKGKEWNPPPAGDAKDRWREYRVYRYDPETGE
                     NPRVDTYWVDMNECGPMILDALLWIKNTVDPTLTFRRSCREGVCGSCSMNVAGTNTLA
                     CTMHADDAKGAAIAIYPLPHMPVVKDLVPDMTQFYAQHRAIEPWLQTDSPTPQKEWRQ
                     SHDDREKLDGLYECILCACCSTACPSYWWNSDRYLGPAVLLQANRWVEDSRDERTGER
                     LDDLEDPFRLYRCHTILNCTKACPKGLNPAAAIANIKRKMVERT"
     gene            complement(1940..2395)
                     /locus_tag="AC16Y7_05535"
     CDS             complement(1940..2395)
                     /locus_tag="AC16Y7_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708875.1"
                     /translation="MRGLSRAKAAVLGALGAAALLTACGGGGSGPEDAIRELNRVDHG
                     KPYIGMSKADVLRCAGQPRSRIPAGTNAETLIYHYSGPGPVPGADIKNAECTASLTMD
                     RGKLARVSYAHMNVRSPYQWQSERDPEKAKELRNTPVPSCTFSLPRCPR"
     gene            2577..3059
                     /locus_tag="AC16Y7_05540"
     CDS             2577..3059
                     /locus_tag="AC16Y7_05540"
                     /inference="COORDINATES: protein motif:HMM:NF014376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5708876.1"
                     /translation="MTAKRIVAALLVIMLASIGGAPEIFAREDVPPPKVEAQHLLTKP
                     LEGEPGKQIDIQVYTFPPGSAVPWHTHPGAHEIEYGLQGTLMIEEEGKPPYPLEAGKT
                     NYLAPDVVHRGWNASKTEPAKIYVVRIKPKDAPLTDLVWPKSKAAEPDVPKAGAYPEP
                     "
     gene            complement(3064..3540)
                     /locus_tag="AC16Y7_05545"
     CDS             complement(3064..3540)
                     /locus_tag="AC16Y7_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708877.1"
                     /translation="MGEKPLAKAAQSHKDAERSVPTDEELDATPPLPAVVWAARFSAY
                     FLALGGIMLITYLFYGFDTDPRSFPPGFRLNPLQAGVNFIWGLVGTAVGFFRPRFATA
                     FVLAFAAFYTLVAGLGTFAGSALGMELGAPAKVYYWIVVALAWAAGLSGLLRKRRA"
     gene            3733..4701
                     /gene="mdh"
                     /locus_tag="AC16Y7_05550"
     CDS             3733..4701
                     /gene="mdh"
                     /locus_tag="AC16Y7_05550"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003567261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR5708878.1"
                     /translation="MARSKIALIGAGQIGGTLAHLAGIRELGEVVLFDVVDGVPQGKA
                     LDLSQSSPVEGYAPKLAGANEYAEIKDADVVIVTAGVPRKPGMSRDDLLEINLKVMEQ
                     VGAGLRKYAPEAFVICVTNPLDAMVWALQKTSGLPRNKVVGMAGVLDSARFRFFLSQE
                     FDVSPQDIHTMVLGGHGDTMVPLIRYSSVAGIPLGDLVKMRWITQKRLDEIVQRTRNG
                     GAEIVGLLKTGSAYYAPAAAALDMAESYLKDKKRVLPAAAYLNGEYGVKGIYAGVPVI
                     ISSKGVERVIELDLSSAEKDAFVHSVESVKALVELCQRIAPNLASS"
     gene            4723..>4896
                     /locus_tag="AC16Y7_05555"
     CDS             4723..>4896
                     /locus_tag="AC16Y7_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012067154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR5708879.1"
                     /translation="MNIHEYQAKDLLKAYGAPVAKGVVAKSADEAAELAKELNGEIYV
                     VKALIHAGGRGKGG"
BASE COUNT          942 a         1617 c         1516 g          821 t
ORIGIN      
        1 tcggcgcata tctgcaccgg cgacggcaac gccatggtgc tgcgcgcagg gctcccgctg
       61 caggacatgg agttcgtgca gttccatccc accggcgttt acggcgccgg cgtgctcatc
      121 accgaaggtg cgcgcggcga gggcggtttc ttgcgaaact ccgagggcga gaagttcatg
      181 gagcgctatg caccgtccgc caaggatctt gcgtcgcgcg acgtggtctc ccgcgccatc
      241 accatcgaaa tccgcgaagg ccgcggccat ggtcccgaaa aggactacat gcatctccat
      301 ctcgatcatc tcgacgagga catgctggcc gaacggctgc caggcatcac cgacatcgcc
      361 cgcatttttg gcggcgtcga tgtgcgcaac gaaccgatcc cgatcattcc gacctgccac
      421 tacaatatgg gcggcattcc taccaatatg catggcgaag tgatcgcccc gcgcggcgac
      481 gacaaagacg ctgtcgtgcc cggcctcatg gcgctcggcg aagcggcttg cgtgtcggta
      541 catggcgcca accgtttggg ctcgaactcg ctgatcgatc tcgtggtgtt cggacgcgcc
      601 gcagcgctgc gttgcgccga gatcgtgggc aaggacaccg caattcccga actgccgaaa
      661 ggcgcgggcg acgaggccat cgcacgcctc gatcgcttcc gctatgccga cggcggcacg
      721 ccgaccgccg agcttcgctt ggcgatgcaa aagacgatgc agcgcgattg ttcagtgttc
      781 cgcactaagg aagtgctcgc cgagggcgtt gagaacatca agaaggtgtg ggacgacgcc
      841 gaggacatcg ccgttaccga ccgctcgctg atctggaaca cggacctcat cgagacgctg
      901 gagttcgaca atctcatcag ccaagccgcc gtcaccgtgc agggcgcgct ggcccgcgaa
      961 gaatctcgcg gcgcgcatgc ccgcgaggac tttccgaagc gtgacgacaa ggactggatg
     1021 aagcatacat tgtcttacgc gagctacggc acgcacacgg tcacgctcga cttccggccg
     1081 gtgcacacct acacgctgtc gaacgaggtc agctacatcg agcccaaaga gcgggtgtat
     1141 tagacatggt ccagttcaca cttcccaaga gctcgaaaat caaaaagggc aaggagtgga
     1201 atccgccgcc cgcgggcgat gcgaaggacc gctggcgcga ataccgcgtc taccgctacg
     1261 acccggagac cggtgagaac ccgcgcgtcg atacctattg ggtcgacatg aacgagtgcg
     1321 ggccgatgat tctcgacgca cttctgtgga tcaagaacac cgtcgatccg acgctcacct
     1381 tccgccgctc ttgccgcgaa ggcgtgtgcg ggtcctgctc catgaacgtc gccggcacca
     1441 acacgctcgc ctgcaccatg cacgccgacg acgccaaggg cgccgcgatc gccatctatc
     1501 ctttgccgca tatgccggtg gtcaaagacc tcgtgccgga catgacgcag ttttacgcgc
     1561 agcaccgcgc catcgaacct tggcttcaga ccgacagccc gacgccgcaa aaggaatggc
     1621 gccagagtca cgacgaccgc gaaaagctcg acgggcttta cgagtgcatc ttatgcgcct
     1681 gctgctcgac cgcctgcccg tcctattggt ggaacagcga ccgctatctc ggcccggccg
     1741 tcctgttaca ggccaatcgt tgggtggagg acagccgcga cgagcgcacc ggtgagcggc
     1801 tcgacgatct cgaggatccg ttccggctct atcgctgcca caccattttg aattgcacca
     1861 aggcctgccc caaggggctc aacccggccg cggccatcgc caacatcaag cgcaagatgg
     1921 tggagcggac ttaaacccgc tagcgcgggc agcgcggtag cgagaacgtg cagctcggca
     1981 ccggcgtatt acgcagctct ttggccttct ccggatcgcg ctcgctttgc cattgatagg
     2041 ggcttcgcac gttcatatgc gcatagctca cccgcgccag cttgccccga tccatggtga
     2101 ggctcgccgt acactcagcg ttcttgatgt ccgcgcccgg cacgggaccg gggccgctat
     2161 agtgatagat cagcgtctcg gcgttcgttc cggccggtat gcgcgagcgg ggctggccgg
     2221 cgcaacgcag cacgtccgcc ttgctcatgc cgatataggg cttgccgtga tcgacgcggt
     2281 tgagctccct gatggcgtcc tccgggcccg acccgccacc gccgcaagcg gtcagcaggg
     2341 cggccgcacc aagggcgccc agaaccgcag ctttggctcg ggaaagtcct ctcatcaggt
     2401 cctttgcccc acgtccacgc acatccatcc cttttcataa gctggaaaag gccctagcgc
     2461 cagattgcga gggctttggg gcacctcgcc ctatccagcc tcagcggcgg cgaggcccct
     2521 tcacatctcg cgaaattcac tgttccgcgc ggagcccggc acgtaggctt gcacccatga
     2581 ccgcaaaacg gattgttgcc gccctccttg tcataatgct ggcaagtatt ggtggcgccc
     2641 ccgagatctt cgcgcgcgag gacgtccccc cgcccaaggt cgaggcgcag caccttctga
     2701 ccaagccgct cgaaggcgag ccgggcaagc agatcgacat ccaggtctac acctttcctc
     2761 cggggagcgc ggtgccgtgg catacccacc ccggcgcgca cgagatcgag tatgggctcc
     2821 agggcacgct gatgatcgag gaggagggca aaccgcccta cccgctggag gccggcaaga
     2881 ccaactatct ggctccggac gtggtgcatc gcggctggaa tgcgagcaag accgagccgg
     2941 ccaagatcta cgtggtccgt atcaagccga aggatgcgcc gctgaccgat ctggtttggc
     3001 cgaaatcaaa ggccgcagaa cccgacgtgc ccaaggcagg cgcctatccg gagccttgag
     3061 ccgtcaggcg cgccgtttgc gcagcagccc tgagaggccg gcggcccagg ccagcgccac
     3121 cacgatccag taatagacct tggcgggtgc gcccagctcc atgccgagcg cggagccggc
     3181 gaacgtgccg agccctgcaa ccagcgtgta gaaggccgcg aaggcgagca cgaaggcggt
     3241 ggcgaagcgc ggccggaaga agccgaccgc ggtccctacc aagccccaga tgaagttgac
     3301 cccggcctgg agggggttca gccgaaagcc cggcgggaag ctccgcggat ccgtgtcgaa
     3361 gccatagaaa agataggtga tcagcatgat gccgcccaac gccaggaaat aggcgctgaa
     3421 ccgcgccgcc cagaccacgg ccggcagagg cggggtcgca tccagctcct cgtccgtggg
     3481 cactgacctc tcagcgtcct tgtggctttg ggcagctttg gccagcggct tctcccccat
     3541 gctcctcgac cccgtggctc cctcaaatct tgcggctcct ctggcagcct agccgattgg
     3601 gcgcccgctt tcatacgatt cgccaccttg cgcgacggcc cgattgcatt taaccaacca
     3661 gcgagttcag gcgggcccga aattgcttca gatgcggccc atttcagtca gcgtattcaa
     3721 cagggggttt tcatggcgcg cagtaagatt gcactgatcg gcgccggcca aatcggcggc
     3781 acgctcgccc acctggccgg tattcgggag cttggtgagg tcgttctctt cgacgtggtg
     3841 gacggcgtac cgcaaggcaa ggcgctcgac ctgtcgcagt cctcacccgt ggagggttac
     3901 gcgccgaaac tcgcaggcgc caacgaatat gccgagatca aggacgccga cgtggtgatc
     3961 gtcaccgccg gcgtgccccg caagcccggc atgagccgcg acgacctgct cgagatcaac
     4021 ctgaaagtga tggagcaggt gggcgcgggg ctccgcaaat acgctccgga agccttcgtc
     4081 atctgcgtga ccaacccgct cgacgccatg gtctgggccc tgcagaagac gtccggtctg
     4141 ccccgcaaca aggtagtcgg catggccggc gtgctcgaca gcgcccgctt ccgcttcttc
     4201 ctgtcccagg aattcgacgt cagcccgcag gacattcaca cgatggtgct tggcggccat
     4261 ggcgacacca tggtgccgct gatccgctat tcgtcggttg ccggcattcc gctcggcgat
     4321 ttggtcaaga tgcgctggat cacgcagaag cgtctggatg agatcgtgca gcgcacgcgc
     4381 aacggcggcg ccgagatcgt gggcctgctc aagaccggct cggcctatta cgcgccggcc
     4441 gcggccgcgc tcgacatggc ggagtcctac ctcaaggaca aaaagcgagt gctgcccgcc
     4501 gccgcttatc tcaacggcga atacggcgtc aaaggcatct atgccggcgt gcccgtgatc
     4561 atcagctcca agggcgtgga gcgcgtcatc gagcttgacc tgtcgtcggc ggagaaagac
     4621 gccttcgtgc actccgtcga gtcggtgaaa gctctcgtcg aactgtgcca gcggatcgcg
     4681 ccaaacctcg cgtcgtcgta agagcgcctt ccggagactt ccatgaacat tcacgagtac
     4741 caggccaaag acctgctcaa ggcctacggc gcccctgtgg ccaaaggtgt cgttgccaaa
     4801 agcgccgacg aagcggccga gctcgccaag gagttgaacg gggagatcta cgtcgtgaag
     4861 gcgctgatcc acgccggtgg acgcggcaag ggtggc
//
LOCUS       DBNHVU010000140        19713 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_498333, whole genome shotgun sequence.
ACCESSION   DBNHVU010000140 DBNHVU010000000
VERSION     DBNHVU010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 19713)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19713)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19713
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_498333"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="AC16Y7_05560"
     CDS             complement(<1..824)
                     /locus_tag="AC16Y7_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HIR5708880.1"
                     /translation="MSDPSSDAPIELAFPDDDLVLPFQAEQADVLGRLVKLGPTVDTI
                     LSRHAYPEPVSRLLGEAVALTALLGASLKSDGKLILQTTTDGPVDLLVADYLVPGALR
                     GYARFDAERLAALDTDDDAALLGQGHLAMTIDRGLDTERYQGVVPLEGESLTEAADTY
                     FRQSEQLPTFIRLAVARHYRAGEGGDEGAWTWRAGGLLVQKLTREGGVETPKEFEEDW
                     SRAKMLAETVEDHELLDPLLPPDRLVYRLFHEETVRAYRHIPLETYCSCSRERVEE"
     gene            complement(821..1882)
                     /gene="argF"
                     /locus_tag="AC16Y7_05565"
     CDS             complement(821..1882)
                     /gene="argF"
                     /locus_tag="AC16Y7_05565"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR5708881.1"
                     /translation="MSPTPKHFLDLDALDASTLRAIVDTAAAMKKSGRVPKALGVPAG
                     AVLAMIFEKPSTRTRVSFGVAIHQLGGTSIALNPQDMQLGRGETVADTARVLSRYVDA
                     IVLRTGPHATLLEFAHNATIPVINGLTARSHPCQVMADVLTFEERKGPIKGRVIAWIG
                     DANNVAASWVHAAARFGFTLRIAAPANFAPAPDLLAWANAEGAKVEVMESAREAAHGA
                     DCVVTDTWVSMGDNDENKRKATLRPFQVDEAIMAAGKRNAIFMHCLPAHRGDEVTAAV
                     IDGPQSAVWEEAENRLHVQKAILAWCLGSGRLGTKSKTRNAKTRSATKKKTRSAEKTA
                     AKKTRTTKTSRTKTRGARR"
     gene            complement(1917..3095)
                     /locus_tag="AC16Y7_05570"
     CDS             complement(1917..3095)
                     /locus_tag="AC16Y7_05570"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009465599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HIR5708882.1"
                     /translation="MSALLPTYARSGLSFARGEGAFLFTETGERYLDFSSGIAVTALG
                     HTHPQLIEALTEQSQKLWHVSNLHHIAGQERLGERLVEASFADKVFFANSGAEAVEAS
                     IKAARRYHFVNGAPERYRLVTFEGAFHGRTLATIAAGGQEKHLEGFGPPVQGFDQVPG
                     FDIDAVAAAIGDETAGVLLEPIMGEGGMREVPWRFLQDLRALCDATGILLVLDEVQSG
                     VGRTGRFYAHEWAGITPDIMASAKGLGGGFPVGACLTTEAVGDAMTPGTHGSTFGGNP
                     LAMAVGNAVLDVVLAPGFLEHVAQMGLRLKQRLASLADEHSDIIAEVRGQGLMVGLRC
                     QVENKTLIAGLRERGMLTVPAGDNVVRLLPPLIVGEDEIDFACTTIDAACAALKSGAS
                     "
     gene            3481..3981
                     /locus_tag="AC16Y7_05575"
     CDS             3481..3981
                     /locus_tag="AC16Y7_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HIR5708883.1"
                     /translation="MAWTDDRVELLKKLWADGLSASQIAGRLGGVTRNAVIGKVHRLG
                     LSGRATSSRSSAPRPRRSHAPRPNRGPGLTFGTRGNVALKPTFEAEYEPVPAPVAELV
                     IPLHERASILTLKESMCHWPIGDPTEEDFHFCGRKKDGPTPYCDHHARMAYQPAQARR
                     RRQAGG"
     gene            complement(4058..4786)
                     /gene="phoB"
                     /locus_tag="AC16Y7_05580"
     CDS             complement(4058..4786)
                     /gene="phoB"
                     /locus_tag="AC16Y7_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HIR5708884.1"
                     /translation="MLQTNEVSGGGAKVLVVEDEEPLALLLRYNLEAEGYAVDVVHRG
                     DEAEIAIAESSPDLVVLDWMLPGISGLELCRRLRTGKESRTIPIILLTARSEETDRIR
                     GLTTGADDYVVKPFSLPELMARVRAILRRTNPERIASVLSVGDIELDRDSHRVTRNGR
                     QVHLGPTEFRLLEFLMQSPGRVFSRAQLLDGVWGRNVYVDERTVDVHIGRLRKAMMRG
                     SEPDPVRTVRGAGYAVSDKLEETT"
     gene            complement(4805..5536)
                     /gene="phoU"
                     /locus_tag="AC16Y7_05585"
     CDS             complement(4805..5536)
                     /gene="phoU"
                     /locus_tag="AC16Y7_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR5708885.1"
                     /translation="MEQQHEHIVRSYEEELALLNNKIAKMGGLAEQVLGQSFEALERR
                     DPDLAAATINEDEAIDDLQREIEEQAVVMIARRQPMAYDLRQIMAALRISTDLERIGD
                     LGKNIAKRAVAVVTEQQPKQLMLGLKHMGELSLSQLKEVLDAFIERDAERALNVWYKD
                     EEIDAMYNSLFRELLTYMMEDPRNIGLCTHLLFGAKNIERVGDHATNIAETVYYLVHG
                     RAITDQRPKGDTTSSTPVKLTRNVV"
     gene            complement(5562..6308)
                     /gene="pstB"
                     /locus_tag="AC16Y7_05590"
     CDS             complement(5562..6308)
                     /gene="pstB"
                     /locus_tag="AC16Y7_05590"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR5708886.1"
                     /translation="MKAADVNLWYGEKQALFDVNLEMFDKSVTALIGPSGCGKSTFLR
                     CLDRMNDVIDICRVQGDIRLDGEDIYAPRVDPVQLRARVGMVFQKPNPFPKSIFENVA
                     YGPRIHGTCRTKAEFEEVVEGSLTRAGLWEEVKDRLDEPGTGLSGGQQQRLCIARAIS
                     VNPEVLLMDEPCSALDPIATAKIETLMDELKENLCIVIVTHSMQQAARVSQRTAFFHL
                     GKLVEVDQTSKIFTNPADQRTQDYVTGRFG"
     gene            complement(6425..8053)
                     /gene="pstA"
                     /locus_tag="AC16Y7_05595"
     CDS             complement(6425..8053)
                     /gene="pstA"
                     /locus_tag="AC16Y7_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR5708887.1"
                     /translation="MSDAQVLTPAGTIDWNSDKARRRVRRRYAADRRLQAYGIIAIAL
                     AVGLLSVLVASLVRTGFPAFLQTKVDLPIYVDASKVDAEDPASGNYRGLIKAALNKAI
                     PDVPKDQQRSVRKILTSDAAYILRDYVVDHPDSIGKTITLPIALSDPFDQLYKGVIPK
                     HMDALNWVQEEWFERLQNRNVVVEHDGRTSLKLDVYVDPAAVEDGDYVDLARNSLQAY
                     FPRVRDSSLFEMLAPDAPAAIEKAVAEDPAIVGKTVQIYFPVSEHYATLAAGEEPTIL
                     NPRFNEAQLAAYYVFVDKGMLRTPFNWELFFNADSRFPERAGLAGAITGSFYALLVCF
                     MLSFPIGIAAAVYLEEFAPKNRLTDLIEVNINNLAAVPSVVFGLLGLAVFLGFFGLPR
                     SAPLVGGMVLSLMTLPTIIITTRAALKAVPPSIREAALGVGASKHQVMLHHVLPLALP
                     GILTGTIIGMAQALGETAPLLLIGMNAFIPSVENMGLLEPATSLPTQIFSWADSPERG
                     FVSRTSAAILVLLGFLVIMNLVAITLRQMFQRKW"
     gene            complement(8046..9425)
                     /gene="pstC"
                     /locus_tag="AC16Y7_05600"
     CDS             complement(8046..9425)
                     /gene="pstC"
                     /locus_tag="AC16Y7_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR5708888.1"
                     /translation="MIGYVLLVLLVLAVVSYFLGRRRAVSAVEGREQDLHSRPSYHGA
                     FVAAWVGLPSILFVLVWLLFQGSVIDNLLLASLPAGTTDGLTAGQTSLLLSEIKSVAA
                     GNIFGEPSATVLEAAERYNRWQTIARWSMFAAAVSIALMGLALAGGRISRDFRARHGA
                     ERIMSGLMIACSLIAMLTTLGIVLSLLIEALRFFDRVSPIEFFFGLNWEPQIPIREGQ
                     VTAGGAFGAIPVFAGTLLIALIAMLVATPIGLYSAIYLTEYAHPRIRAVIKPILELLA
                     GIPTVVYGFFAVLTVAPAMRQFGEMIGMPIAPNSALAAGAVMGIMIIPFISSLSDDAI
                     AAVPRAMRDGSYAMGATKGETITKVLLPAALPGIMGGVLLAVSRAIGETMIVVMAAGI
                     IATLTANPLKPVTTVTVQIVTLLIGDTEFDNPKTLAAFALGLVLFLVTLALNVLALHI
                     VRKYREQYE"
     gene            complement(9580..10638)
                     /locus_tag="AC16Y7_05605"
     CDS             complement(9580..10638)
                     /locus_tag="AC16Y7_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR5708889.1"
                     /translation="MNRIAIAASTLAVAALCTGGPALARDQIKIVGSSTVFPYTQAVA
                     EEFGKKSGKKSPVVESTGTGGGMKIFCQGIGEDKPDITGASRAMKKSEYELCAKNGVD
                     DITEVLIGYDGLSIAQSNKGGPMDLSKEQIFLALSSEVPDGDKLVPNPNKKWSDIDKS
                     LPDVAITAYGPPPTSGTRDAFVELGMHEGCKGLDYFKKQKGELDKKAFKSLVKEKCTP
                     MRQDGPFIEAGENDNLIVQRLEADPNAVGIFGYSFLYENQDKLNGVKVGGTKPGFDTI
                     ADGSYGLSRPLFIYIKNPHRKVISGMNEFVTEYVSDEAMGPDGYLNERGLVVLPDDKL
                     KQMQDAAKDGTKMAAPTS"
     gene            complement(10828..12186)
                     /locus_tag="AC16Y7_05610"
     CDS             complement(10828..12186)
                     /locus_tag="AC16Y7_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5708890.1"
                     /translation="MSNQVEHSARPIASGLLWRASQALRRGTRELATRLWAALWQIVI
                     VAVIMASLVTLGDLSVEIALAAFASFVGAVALLPRQDVDSALDRQFPPSPSLTRLNSP
                     MHVMSEALPDPVILLDRAGQVLSCNAPARGLFTSLREGIHISSVIRTPEFLDAVSAAP
                     RVGEAVTVIYAQRVPVGRRMAVTVAPLARGSESDGSILVLLRDLTEVERINQMRADFI
                     ANASHELRTPLASLRGFIETLQGTAKQDPGARERFLPIMAEQASRMTRLIDTLLSLSR
                     VEMNAHLPPSDRIDLNDVLGHARDTLEPIAADSGMELDVQRFRQPAIVRGDRDELLQV
                     LQNLVQNAFKYGSPGGHVRIEAKHIPTLGRQVAGRFVISVVDDGPGIPAEHLPRLTER
                     FYRVDVESSREKGGTGLGLAIVKHILNRHHGELAIASDPGNGSSFTIMLNESRVNEAR
                     AS"
     gene            12492..13592
                     /gene="ada"
                     /locus_tag="AC16Y7_05615"
     CDS             12492..13592
                     /gene="ada"
                     /locus_tag="AC16Y7_05615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HIR5708891.1"
                     /translation="MSAAPMSAPAPQRLEPAGRLSDEACWEAVLGRDARCDGHFYYSV
                     DTTGIYCRPSCPAKRPKRAHVRFHDTVEKAEAAGFRACKRCRPGQPSLAAQHSARIAQ
                     ACRMIEAADTPPPLAALADAVGLSAYHFHRFFKAALGVTPKAYASALRNKRVREGLPQ
                     TTTVTEALYDAGFNSSGRFYATATEALGMSPRTYRDGGDGTEIRYATGTCSLGAILVA
                     ATDKGVCAIFLGDAPQDLVVELHERFPRAALAEGDAGFAALTARVIATVEGPSAQIDL
                     PLDIKGTAFQLRVWEALRRIPPGTTASYAEIAASIGAPEAARAVAGACAANKIAVAIP
                     CHRVVGADGTLSGYRWGVARKRALLGKEKSGN"
     gene            complement(13605..13955)
                     /locus_tag="AC16Y7_05620"
     CDS             complement(13605..13955)
                     /locus_tag="AC16Y7_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase family protein"
                     /protein_id="HIR5708892.1"
                     /translation="MTAWPTELKLDKDKRTLIVSFDDGASYDLPAEVLRVLSPSAEVQ
                     GHSPEQRITVPGKKNVRISRLEPVGNYAVRIVFDDGHDTGLYVWEYLHDLGENQEPRF
                     RSYLEELAEKGLTR"
     gene            complement(13965..14153)
                     /locus_tag="AC16Y7_05625"
     CDS             complement(13965..14153)
                     /locus_tag="AC16Y7_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6494 family protein"
                     /protein_id="HIR5708893.1"
                     /translation="MDEQQFNMSLRRFLKEVGVTSQQAIERVVREGDLGGKLKVRMVL
                     TAKGTSLDHVVEGEIDLG"
     gene            14243..14440
                     /locus_tag="AC16Y7_05630"
     CDS             14243..14440
                     /locus_tag="AC16Y7_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708894.1"
                     /translation="MAVTSQGKGRTTKTRAGAMKQKRTVKSRNAPAKALADPRYRPRV
                     VKGAKVYSRKDQKTPGEDDGA"
     gene            14441..15457
                     /gene="moaA"
                     /locus_tag="AC16Y7_05635"
     CDS             14441..15457
                     /gene="moaA"
                     /locus_tag="AC16Y7_05635"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016733252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR5708895.1"
                     /translation="MSELEDELAPPLIDRFGRAITYLRVSVTDRCDFRCTYCMAEDMT
                     FVPRKELLALEEIDRLASAFIARGVRKVRLTGGEPLVRRNILWLIEALSRHLGDGLEE
                     LTLTTNGSQLYRFADALADLGIKRINVSLDTLDAAKFRVLTRWGDFDQVMRGIDAADR
                     AGLKIKINTVALRDMNEDELEDLLRWAHGRGFDLTLIETMPLGEIDTDRTDQYLPLSL
                     VHSRLSQHLTLTDIPHRSGGPARYMQVAETGGRLGLITPMTHNFCESCNRVRVTCTGT
                     LYMCLGQDDAADLRAPLRASTSGAPLHAAIDEAMARKPKGHDFIIDRDARPAISRHMS
                     ATGG"
     gene            15616..17625
                     /locus_tag="AC16Y7_05640"
     CDS             15616..17625
                     /locus_tag="AC16Y7_05640"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR5708896.1"
                     /translation="MIWLQTRDWPRFAHMLFKDLFKKYWHWAGLALLSALIVSTAFIA
                     PGRVSLLELEQEALVAQDRIHTQMLRDPQVVHNALNVPGAAPQLGEIFHASGYAPRVL
                     RYEVYNAKGRLLFTSGQANLRLDGPPLNVTNNATQQASVVSLQSGAGNNDPSNFAVLR
                     VPINFAANERGTLIVYLDQSDQASVLSSYFSVIALVTLTLLGVGVTMPAAFAWMRSRE
                     RRQAEEQVHYLQNHDALTGLANRRAFAERLGDATARMKQDDTHIAVICLDIDKFKEIN
                     DSTEHGGGDQVLREIGVRINETLRDRDIIARLGADEFAIALVDVSNLGDVMAFMNRLV
                     AALQLPFRIADKEILITTSVGIALAPADGDTASALLRHATIALSRAKGDGGQRMCFFE
                     ESMDKALQRRRTVEHELRLALGREEFEVAYPPQFDLATEEQCGSEALIRWHHPLHGKI
                     APKHFISVAEETGLIVPIGEWVLRRACCDAVSWPEHLTVAVNLSPAQFRDRDIAETVA
                     DILHETGLPAERLELEITENLLINDTEEVLGKLNRLRKIGVKIAMDDFGTGYSSLSYL
                     ARFPFSKIKIDRQFIRNMARDPAMRAIVRTIIALGKSLNVTITAEGVETPEQAKILSE
                     FGCGEVQGFLYGRPEAAQPYAKLEQSADGKVTPIRARRRRRPSAA"
     gene            complement(17654..18445)
                     /locus_tag="AC16Y7_05645"
     CDS             complement(17654..18445)
                     /locus_tag="AC16Y7_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708897.1"
                     /translation="MLHDEKPSLSMCAFGAGAAHAALLALALPIMITLPAPADKAQGT
                     VAVQVVVRSTPPPTLETAMAETAALPLPEYIGEGDIDEDALPASVLDAVLGASPGSDL
                     DAMPASAVGEITGSLSQAFASPDVLQTQAALLEVIATAPPPRTVEPAALPLVESVPAP
                     DADGGIDGEAIASIESKEVIWSQEVPEASPAIVPVPLRKPSAATVVAPAPPVARKPVR
                     KARVKPRSRGNAQKRTAPVQAKSPNKGFLGGRQAAPMAEYPLANR"
     gene            complement(18536..19708)
                     /gene="apbC"
                     /locus_tag="AC16Y7_05650"
     CDS             complement(18536..19708)
                     /gene="apbC"
                     /locus_tag="AC16Y7_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009449293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HIR5708898.1"
                     /translation="MKGPDLKDDIVSLGLVSEVVIHKGKVYFAISIDPARARELEGLR
                     QAAETVVKDLPGVEGVTVTLTADREPGSGSVSPVASNGGARQAPPPQSSPAQASPPQA
                     PQGPGATAPGAARDARHRPGGVPGVKHIIAVASGKGGVGKSTTAVNLALALKDRGLEV
                     GVLDADIYGPSMPRLLGIQGQPQQLAGNKLDPMRAYGLKIMSMGFLVDEDTPMIWRGP
                     MVMSALSQMLKDVAWGELDLLVVDMPPGTGDAQLTMAQQVPLAGAVIVSTPQDLALID
                     ARKGLNMFTKVNVPVLGIVENMSTFLCPHCGERSDIFGHGGAREEANKLGVPFLGEVP
                     LTMAVRETSDAGKPVVATDPDGEPAKAYCGIAAKAWAELERHKDADRPAPKIVMEN"
BASE COUNT         3692 a         6254 c         6220 g         3547 t
ORIGIN      
        1 aattcctcca cgcgctcccg tgagcaactg caataggtct ccaacggaat gtgccgatag
       61 gcgcgcaccg tctcctcatg aaagaggcgg tagaccagcc ggtcgggcgg cagcagcgga
      121 tccagcaatt cgtggtcttc gacagtctct gccagcatct tagcgcggga ccaatcttcc
      181 tcgaattcct tcggggtctc gacgccgccc tcgcgggtca gcttctgcac gaggagcccg
      241 ccggcccgcc aggtccaggc gccttcgtcg ccgccctcgc ctgcacgata gtggcgcgcc
      301 acggcgaggc gaatgaaggt cgggagctgc tccgactggc ggaaataggt gtcggcggct
      361 tccgtgaggc tctcgccttc cagcggcacg acaccttgat agcgctcggt atcgagcccc
      421 cggtcgatgg tcatggccaa atggccctgg ccgagcagcg cggcatcgtc gtccgtgtcg
      481 agcgccgcca gccgctccgc atcgaaacgc gcatagcctc gcaaagcgcc gggcacgaga
      541 taatccgcca cgagcagatc gaccggcccg tcggtggtgg tttgtaggat cagcttgccg
      601 tcggatttga gcgaggcgcc gagtagggcc gtcagcgcca cggcctcacc gagaagacgc
      661 gacacgggtt cggggtaagc gtggcgcgac aggatggtgt ccacggtcgg cccgagcttg
      721 accaaacggc cgagcacgtc ggcttgctcc gcttggaacg gcaggactag gtcgtcgtca
      781 ggaaacgcaa gttcgatggg cgcgtccgag gagggatcgc tcaccgcctg gcaccgcgtg
      841 ttttggtgcg cgaggtcttg gtggtgcgcg tcttcttggc ggcggtcttc tcggcgctcc
      901 gcgtcttctt cttcgtggcg ctgcgtgtct tcgcattgcg cgtcttgctc ttggtgccaa
      961 gccggccact gcccaagcac caggccagaa tggccttctg cacatgcagc cggttctccg
     1021 cctcctccca cacggcggac tgcgggccgt cgatcaccgc ggccgtcact tcatcgccgc
     1081 gatgggcggg aaggcagtgc atgaagatcg catttcgttt gcctgcggcc atgatcgctt
     1141 cgtctacttg gaagggacgg agcgtggcct tgcgcttgtt ctcatcgttg tcgcccatgg
     1201 acacccaagt gtcggtgaca acgcagtccg cgccgtgtgc cgcctcccgc gcactctcca
     1261 tgacttcaac cttggcccct tcggcattcg cccaggccag cagatccggc gccggcgcga
     1321 agttggccgg cgcagcgatg cgcaaggtga aaccgaaccg cgctgcagca tggacccatg
     1381 acgcggcgac attgttggca tcgccaatcc aggcgatcac gcggcccttg atcggccctt
     1441 tgcgctcctc gaaggtcaag acgtcggcca tgacctggca gggatgggag cgcgcggtga
     1501 gaccgttgat caccgggatc gtcgcgttgt gcgcaaattc cagcagcgtc gcatgcggcc
     1561 ccgtgcgcag cacgatggcg tcgacatagc gcgacaggac ccgcgcggtg tcggcgaccg
     1621 tctcgccccg ccctagctgc atgtcttgcg gattgagcgc gatggacgtg ccgccgagct
     1681 gatggatggc gacgccgaag gaaactcgcg tacgcgtgga cggcttctcg aaaatcatgg
     1741 caagaaccgc cccggccgga acgccgagcg ctttcggcac ccggccggac tttttcatcg
     1801 cggcggcggt atcgacgatg gcgcgcaagg tggatgcgtc caatgcgtcg agatcgagga
     1861 aatgcttagg ggtcggtgac atgggtgaac cgtctcgaaa ggaaagctag ggccgcttag
     1921 gaagcgccgg acttcagcgc ggcgcaggcg gcgtctatcg tggtgcaagc aaagtcaatc
     1981 tcgtcctcgc cgacgatcag gggcggcaag aggcgcacga cattgtcgcc ggccgggaca
     2041 gtgagcattc cccgctcgcg caagccggcg atgagtgtct tgttttcgac ttggcatctc
     2101 aagccgacca tcagtccctg gccccgcacc tcggcgatga tgtcgctatg ctcatcggct
     2161 aggctggcaa gccgttgttt caaccttagg cccatctgcg cgacatgttc gagaaagccg
     2221 ggggccaaaa cgacatcgag cacagcattg cccacagcca tggcgagcgg attgccgccg
     2281 aaggtcgagc cgtgcgtgcc gggcgtcatg gcatcgccca ccgcttcggt ggtgaggcac
     2341 gcgccgacgg gaaagccgcc gccgagccct ttcgccgagg ccatgatgtc gggcgtgatg
     2401 ccggcccatt catgcgcgta gaagcgcccc gtgcggccaa cgcccgattg gacctcgtcc
     2461 agcacgagaa gaatccccgt cgcgtcgcag agcgccctta agtcctgcaa gaagcgccag
     2521 ggcacttccc gcatgccacc ctcgcccatg atcggttcaa gcagcacgcc ggcagtctca
     2581 tcgccgatgg cggccgcgac ggcatcgata tcgaagcccg ggacctgatc gaagccctga
     2641 acgggcgggc cgaagccttc cagatgcttc tcctgaccgc ccgcggcaat ggtcgccagg
     2701 gtacggccgt gaaaggcgcc ctcgaaggtg acgaggcgat agcgttcggg cgcgccgttg
     2761 acaaaatggt agcgccgcgc ggccttgatc gaggcctcga cggcctcggc gccggaattg
     2821 gcgaagaata ccttgtcggc gaagctggcc tcaacgagac gctcccccag ccgctcctgt
     2881 ccggcgatgt gatgcagatt ggagacgtgc cagagcttct gcgactgctc cgtcagcgcc
     2941 tcgataagct gcgggtgcgt gtggccgagc gcagtcaccg cgatcccgct ggagaagtcg
     3001 agataacgct cacccgtctc ggtaaagaga aaggcacctt cgccccgcgc gaaggacaat
     3061 ccgctgcggg cataggtcgg aaggagggcc gacatgaaag ttgcgtatcc ctgaaacaaa
     3121 attgccgccc aagggcggca gcaagcgaag tcaaagtgtt aggtgtcgcg aacttgcctg
     3181 tcaatcgaca ctaacaaccg cttttccaag gtttctccac gtctcaaacg ccccgcaggt
     3241 ttgtgttctc aaggcggaag tggcaaaatc cgcttgttcc ggtggaaaac ggggcaccca
     3301 agcgtggcgg acgcttgtcg gagtccaccg ggtttagtag ggtaataatc gctcggctac
     3361 tagatgtcgt gggtgctaca gtgcccgagc ggtcattctc gccagtattt ccgagtaatt
     3421 cgaaaacaaa agccgtctca cgggacgggt ggaatctgag tccagacgag gaggccaatc
     3481 atggcttgga ctgacgatag ggtggaactg ctgaagaaac tctgggcgga cggactgagc
     3541 gcgagccaaa tcgcgggtcg cttaggcggc gtgacccgca atgcggtcat cggcaaggtt
     3601 catcggcttg ggctttcggg acgcgcaacg agttcgcgat cgagcgcgcc acggccacgg
     3661 cgcagccacg caccgcgacc aaatcgcgga ccgggactga cgttcggaac gcggggcaat
     3721 gtcgcgctga agccgacctt cgaggcggag tacgaaccag tgccggcccc ggtagcggag
     3781 cttgtgatcc cgctgcatga gcgggccagt atccttacgc tcaaggagtc gatgtgccac
     3841 tggccgatcg gcgatccgac cgaggaagac ttccacttct gcggacgcaa gaaggacggc
     3901 cccacgccat attgcgatca ccatgcgcgc atggcgtatc aaccggcgca agcacggcgg
     3961 cggcggcaag caggcggcta aggatcggct gacaggaaca cgaataaagg cgccggagga
     4021 tccggcgcct ttttcatttc agcgggcgga aacttttcta agtagtctct tcgagcttat
     4081 cggagacggc gtagcccgcg ccccgaacgg tacgaaccgg atcgggctcg ctcccgcgca
     4141 tcatggcctt gcgtaaacgg ccgatatgca catcgaccgt ccgctcgtcc acgtagacat
     4201 tccgccccca cacgccgtcc aagagctgcg cgcggctgaa cacgcggccg gggctctgca
     4261 tgagaaactc cagaaggcgg aactccgtcg gtccgagatg cacctggcgt ccgttgcggg
     4321 tcacgcggtg ggagtcccga tcgagctcga tatcgccaac ggacagcacc gaggcaatgc
     4381 gctccggatt ggtccgccgc aggatggccc ggacgcgcgc catgagttca ggcagcgaga
     4441 acggcttgac cacatagtca tcggccccgg tggtcaggcc gcgaatgcga tcggtttcct
     4501 cgctgcgggc ggtcagcagg atgatgggga tggtccggga ttccttgccc gtgcgcaaac
     4561 ggcggcaaag ctccagcccg gagatgcccg gcagcatcca atccaaaaca acgagatcgg
     4621 gcgagctctc ggcaatggca atctctgcct cgtcgccacg atggacgaca tcgactgcat
     4681 agccttccgc ttccaggttg tagcgcagga gaagggccag tggctcctcg tcctcgacaa
     4741 ccaggacctt cgctcctccg ccgctcactt cattcgtttg tagcatcggc tcgaccggta
     4801 tcagttagac gacgttccgc gtgagcttca ctggcgttga gctcgtggtg tcgcctttgg
     4861 ggcgctgatc ggtgatggcc cggccgtgca ccaggtaata gaccgtctca gcgatgttgg
     4921 tggcatgatc gcccacgcgc tcgatgttct tggcaccgaa caacagatgg gtgcagagcc
     4981 cgatattgcg cgggtcctcc atcatgtagg tcagcaactc gcggaacagc gagttgtaca
     5041 tggcgtcgat ctcttcgtcc ttgtaccaaa cattcaatgc acgctcagcg tcgcgctcga
     5101 tgaacgcatc gagcacttcc ttgagctggc tcagggaaag ctcgcccata tgcttcaggc
     5161 ccagcatgag ttgcttgggc tgctgctcgg tcaccacggc aaccgcacgc ttggcgatgt
     5221 tcttgccgag atcgccgatg cgctccagat cggtggagat gcgcaacgcc gccatgatct
     5281 gacgaaggtc gtaggccatg ggctggcggc gggcgatcat caccaccgcc tgctcttcga
     5341 tctcgcgttg aagatcatcg atggcctcgt cctcgttgat cgtcgcggcc gcaagatctg
     5401 ggtcgcggcg ctccagcgcc tcgaaggact gcccgagcac ttgctcggca agcccaccca
     5461 tcttggcgat cttgttgttg agaagcgcaa gctcctcctc gtaagacctg acgatgtgtt
     5521 cgtgctgctg ttccattgag gaaactcctg aagagccggt atcagccgaa acggcccgtg
     5581 acgtaatctt gcgtccgctg gtcagccgga ttggtgaata tcttggacgt ctgatcgacc
     5641 tcgacgagct tgccgagatg gaagaaagcc gtgcgctggg agacgcgcgc cgcttgctgc
     5701 atcgagtggg tcacgatgac gatgcacagg ttctctttga gttcgtccat cagcgtctcg
     5761 atcttggccg tggcgatcgg gtcaagggcc gagcacggct cgtccatcag cagaacctcg
     5821 gggttcaccg agatggcgcg ggcgatacac agccgctgct gctggccgcc ggaaaggccg
     5881 gtgccgggct cgtctagacg atccttcacc tcttcccaga gcccagccct cgtcaggctg
     5941 ccctccacga cctcttcgaa ctcggccttc gtcctgcacg tcccgtgaat ccggggaccg
     6001 tatgcaacgt tctcgaaaat ggatttgggg aaggggttcg gcttttggaa gaccatgccg
     6061 acgcgggccc ggagctgcac cggatcgacg cggggggcgt agatgtcctc accgtcgagc
     6121 cggatgtcgc cttggacgcg gcaaatatcg atcacgtcgt tcatgcgatc gagacagcgc
     6181 aggaacgtgg acttgccgca gccggacggg ccgatcagcg ccgtgaccga cttgtcgaac
     6241 atttcgaggt tcacgtcgaa caaggcctgc ttctcgccat accaaaggtt gacgtcggcc
     6301 gccttcatct tcgggtcgcc ctgctcagca ggcgcttcgc ctcctgtctt ttccggcact
     6361 gcaggcgcct cgacctggat ggatggtcgc tcttccacct tcgcctccaa aactatgtcc
     6421 tcttctacca ctttctttgg aacatctgtc tcagcgtgat cgcaacaaga ttcatgatca
     6481 ccaggaagcc gagcagcacc aggatcgccg ccgaagtgcg cgagacaaag ccacgctcgg
     6541 gactgtcggc ccagctgaag atctgggtcg gcagcgaggt cgctggctcg agaagtccca
     6601 tattctcgac actcggaatg aacgcattca tgccgatcaa gagcagcggc gccgtctcgc
     6661 ccagtgcttg ggccatgccg atgatggtgc cggtcagaat gcctggcaac gccaagggca
     6721 acacgtgatg cagcatgacc tgatgcttgg aggcgccgac acccagcgcc gcctcgcgga
     6781 tggacggcgg caccgccttc agcgcggcgc gcgtggtgat gatgatggtc ggcaaggtca
     6841 tgaggctgag caccataccg ccgacgagcg gggccgaacg cggcaggccg aagaagccca
     6901 ggaacactgc aagaccgaga agaccaaaca ccaccgacgg cacggccgcc aaattgttga
     6961 tgttgacctc gatgaggtcg gtgaggcggt tcttcggcgc gaactcttcc aagtagaccg
     7021 ccgcggcgat gccgatgggg aagctcagca tgaaacagac gaggagcgcg taaaacgaac
     7081 cggtgatcgc cccggcgaga cccgcgcgct ccgggaagcg gctgtccgcg ttgaagaaga
     7141 gctcccagtt gaatggcgtg cgcaacatcc ctttgtcgac aaaaacgtag tacgcggcga
     7201 gctgagcttc gttgaaacgc ggattgagaa tcgtcggctc ttcgcctgca gccagcgtgg
     7261 cgtagtgctc agacacgggg aagtagatct ggacggtctt accgacaatg gccgggtcct
     7321 cggcgacggc tttctcgatg gccgcaggcg cgtccggcgc caacatttcg aacagcgaag
     7381 aatcccgcac gcgcgggaag taggcctgca ggctgtttcg cgccaagtcg acgtagtctc
     7441 cgtcctcgac cgcggccgga tcgacgtaga cgtcgagctt gaggctggtc cgcccgtcgt
     7501 gctcgacgac cacgttccgg ttctgaagtc gctcaaacca ctcttcttgg acccagttca
     7561 gagcgtccat gtgcttcggg atgacgcctt tgtagagctg atcgaaggga tcggagagcg
     7621 cgatgggcaa ggtgatggtc ttgccgatcg agtcggggtg atcgacgaca tagtcccgca
     7681 agatataggc cgcatccgag gtcaggatct tgcggacact gcgctgctga tccttgggaa
     7741 cgtcgggaat ggccttgttg agcgccgctt tgatcaaccc gcggtaattg ccgctggccg
     7801 gatcctctgc atcgaccttg cttgcatcaa catagatcgg caggtcgacc ttcgtttgca
     7861 ggaaggccgg aaaacccgtc ctgaccagcg aggcaacgag tacgctgaga aggccaacgg
     7921 ccagggcgat ggcaatgatg ccgtatgcct ggaggcgcct gtcggccgcg tagcgcctgc
     7981 gaacgcgccg ccgggccttg tccgagttcc agtcgatcgt cccagcggga gttagaacct
     8041 gtgcgtcact catattgttc tcgatatttg cggacgatgt gcagggccag gacgttgagc
     8101 gccaacgtga ccaggaacag cacgaggcca agggcaaagg ctgcaagagt cttcggattg
     8161 tcgaactcgg tatcgccgat cagaagcgtc acgatctgca ccgtcacggt ggtgaccggc
     8221 ttgagcgggt tggccgtgag ggtcgcaatg atgccagcgg ccatcaccac gatcatcgtc
     8281 tcgccgatgg cgcggctgac ggccaaaaga acgccgccca tgatgccggg caacgccgca
     8341 ggcaagagca ccttggtgat ggtctcacct ttggtcgccc ccatggcgta ggagccgtcg
     8401 cgcatggccc gcggcaccgc ggcgatcgca tcgtcggaca gcgacgaaat gaagggaata
     8461 atcatgatgc ccatcaccgc cccggcagcg agggcactgt tgggagcgat cggcatccca
     8521 atcatctcgc cgaactggcg catggccggc gcgacggtca gaacggcgaa gaagccgtag
     8581 accacggtcg gaatgccggc cagtagttca aggatcggct tgatcacggc acggatcctg
     8641 ggatgggcgt actcggtcag gtagatggcc gagtagagcc cgatcggcgt cgccaccagc
     8701 atggcgatca gcgcgatgag cagcgtgccg gcgaagaccg ggatggcgcc gaatgcgccg
     8761 cccgccgtga cctggccttc gcggatcgga atttgcggtt cccagttgag gccgaagaag
     8821 aactcgatcg ggctgacccg atcgaagaag cgcaaggcct cgatcaaaag cgacagcaca
     8881 atgccgagcg ttgtcagcat cgcgatgagc gagcacgcga tcataaggcc gctcatgatg
     8941 cgctcggcgc catggcgggc ccggaagtca cggctgatgc gcccgcccgc gagcgccagc
     9001 cccatcaggg caatgctgac ggcagcggcg aacatgctcc agcgggcgat ggtctgccag
     9061 cgattgtaac gctctgccgc ctctaagacg gtcgccgatg gctcgccgaa aatattgccg
     9121 gcggcaaccg acttgatctc gcttaacagc aggctggtct gccccgctgt caggccatcg
     9181 gtcgtccccg ctggcaggct cgccaacagc aggttgtcga taacgctccc ttggaacagg
     9241 agccaaacaa gaacaaaaag aatggatggg aggcctaccc aagcggcgac gaaggcgccg
     9301 tgatagctcg gccgggaatg gaggtcctgt tcgcggccct cgaccgcgga aacggcccgg
     9361 cgccggccaa ggaaatagct gacgaccgcc agcacgagga gcacgagaag aacataccca
     9421 atcattgtcg cctgccgctc cccgacggac gcacctccct tagtggaggc ctatccgaga
     9481 aaaatacgtt gttgcccaag atcggtaggc gtggccgttc agtcgccaac ggccacgctc
     9541 ttcgattgcg tcatgcgaag agatgaacca cccggcccct tacgaggtcg gtgcagccat
     9601 cttcgttccg tccttcgcgg cgtcctgcat ctgcttcagc ttatcgtccg gaagcacgac
     9661 caagccgcgc tcgttgagat agccgtcagg acccatggcc tcgtcgctga catactcggt
     9721 aacgaactcg ttcatgcccg agatgacctt acgatgcggg ttcttgatgt agatgaagag
     9781 cggacgggac aggccatagc tgccgtcggc gatggtatcg aagccgggct tggtgccacc
     9841 aaccttcacg ccgttgagct tgtcctggtt ctcgtagagg aacgagtagc cgaagatgcc
     9901 aacggcattc ggatcggctt caagccgctg cacgatcagg ttgtcgttct cgccggcctc
     9961 gatgaagggg ccgtcctgac gcatgggggt gcacttctct ttgaccaaag acttgaaagc
    10021 cttcttgtcg agctcgccct tctgcttctt gaagtagtca agacccttgc agccctcatg
    10081 catgccaagc tcgacgaatg catcgcgcgt acctgaggtc ggcggcgggc cgtaggcggt
    10141 gatcgccacg tccgggaggg acttgtcgat gtcgctccac tttttgttgg ggttggggac
    10201 gagcttgtcg ccgtccggaa cctccgagga cagagcgagg aagatctgct ccttgctgag
    10261 atccatcggc ccgcccttgt tggactgggc aatagaaaga ccgtcatagc cgatgagcac
    10321 ctcggtgatg tcgtcgacac cgttcttggc acacagctca tactcggact tcttcatggc
    10381 gcgggatgca cccgtgatgt ccggcttatc ctcgccgatg ccttggcaga aaatcttcat
    10441 gccgccgccg gtaccggttg attcgaccac cggcgatttc ttgccggact tcttaccgaa
    10501 ttcctcggca acagcttggg tgtagggaaa aaccgtcgag gatccgacga tcttgatctg
    10561 gtcacgggca agcgccgggc cgcccgtaca tagagcggcg acggcaagcg tcgacgctgc
    10621 aatagcaatc ctgttcactg tgctctcctt gtcatggatt tgagcgattt gagcctccgc
    10681 tagcgcgagc tacgccgcca gctctgcggc acgctagtcc ccagtgttaa ttgttttatg
    10741 acagcgatga ttcggcttta tgacagtcta aatcactgat tttgttgatt tttggagaga
    10801 gtccgctgtg gaaaacgcga acggccgtca gctcgcgcgc gcttcgttaa cgcgcgactc
    10861 gttgagcata atcgtgaagg aggaaccgtt gcccggatcg gaggcgatcg ccagctcgcc
    10921 gtgatggcgg ttcaagatgt gcttgacgat ggccagaccc aggcccgtgc cacccttttc
    10981 gcgactggac tccacgtcca cccggtaaaa gcgctccgta agccgcggca aatgctcggc
    11041 cgggatgccc ggcccgtcgt ccaccacgga gatgacaaag cggcccgcga cctgacgccc
    11101 gagcgtggga atgtgtttgg cctcgatccg gacgtgaccg ccgggactgc cgtatttgaa
    11161 ggcgttctgc acgagatttt gcagcacctg taagagttcg tcgcggtcgc cgcggacgat
    11221 ggccggctgg cggaagcgct ggacatcaag ctccatgccg ctgtcggcgg caatcggctc
    11281 gagcgtatcc ctggcgtgtc ccaggacgtc gttgagatcg atccgatcgc tcggcggaag
    11341 gtgggcattc atctccacgc ggctgagaga tagcagcgtg tcgatcaggc gggtcatgcg
    11401 cgaggcctgc tcagccatga tcggcaggaa gcgctcgcgg gcgccgggat cttgtttagc
    11461 cgtgccctgc aaggtctcga taaagcccct gagcgatgcg aggggagtcc gcagctcatg
    11521 gctcgcattg gcgataaaat ctgcccgcat ctgattgatg cgctccactt ccgtgagatc
    11581 gcgcaggagc acgaggatac tgccgtcgct ctccgagccg cgggccagcg gcgccacggt
    11641 taccgccatg cggcggccta ccggaacacg ctgcgcatag atgacggtca ccgcttcgcc
    11701 cacgcgcggc gcggcactga cagcgtcgag aaactccggc gtgcggatga cggaggagat
    11761 gtggattcct tcgcgcaaag aggtgaacag tccccgcgcg ggcgcattgc aggacagcac
    11821 ctggcccgcg cgatcgagca ggatgacggg atcgggcagc gcctcggaca tgacatgcat
    11881 gggcgagttc aagcgggtca gtgacggaga cggcgggaac tgccgatcga gtgccgagtc
    11941 cacatcctgg cgcggaagca gggccacggc gccgacgaaa ctggcgaaag ccgcgagtgc
    12001 aatttccaca gacaggtcgc cgagagtcac gagcgaagcc atgatcacgg ccacgatcac
    12061 gatctgccat agagccgccc acaggcgcgt ggcgagctcc cgcgtgcccc gccgaagagc
    12121 ctggctcgcg cgccacagaa ggccggatgc gatgggccga gcgctatgtt ctacttggtt
    12181 gctcaatggc ttgacctacg agccccaacg aatcgccccg gattgggggc gcctatgatt
    12241 gcccctcgaa tatcacgttt tttagacatg tcgccgccgg ttgtacgaca cagtgatgac
    12301 agcgcagtcc gatcgtcatc tatcctgcca gcccttcacg agatgtgggg cgccgaatgg
    12361 gcaataccaa gaccgccgcg ctatcatcca tcgtttgaaa gcaaggatcg gggtgcggcg
    12421 ctcaccagaa gccgccatag tctggacagc gactgacctc gaaggacact ggaccgaagg
    12481 acactggacc catgtctgcc gcgcccatgt ctgcgcctgc gccgcaacgg ctcgaacctg
    12541 ccggccgcct ctccgacgag gcctgctggg aggccgtgct cggccgcgat gccaggtgcg
    12601 atgggcactt ctattactcg gtcgacacga caggcatcta ttgccggccc tcctgccccg
    12661 cgaaacggcc gaaacgggcg catgtgcggt ttcacgacac ggtggagaag gccgaggccg
    12721 cgggcttccg ggcatgtaag cgctgccgtc cgggtcaacc ttcactggcc gcccagcata
    12781 gtgcccgaat cgcacaagcc tgccggatga tcgaggcggc cgacacgccc ccgccgcttg
    12841 ccgcgcttgc ggacgcagtc ggcctcagcg cctatcactt ccaccgcttt ttcaaagcgg
    12901 cgctaggcgt gactccaaaa gcctacgcct cggcgctgcg caacaagcgc gtgcgcgagg
    12961 gcctgcccca aaccacgaca gtgacggagg cgctgtacga tgcgggcttc aattcatccg
    13021 gccgcttcta tgccacggca acggaggctc tcggcatgag tcccagaacg tatcgcgacg
    13081 gtggcgacgg caccgagatc cgctacgcca ccgggacctg ttcgctgggc gcgatactcg
    13141 ttgccgcgac ggacaagggc gtgtgcgcga tatttctggg cgatgcgccc caagacctcg
    13201 ttgtggaact gcacgagcgt ttcccgcgcg ccgcgcttgc cgaaggcgat gcaggcttcg
    13261 ccgcgctcac cgccagggtc atcgctaccg tcgagggccc aagcgcacaa atcgacctgc
    13321 cactcgacat caaggggacg gcgttccagc tacgcgtgtg ggaggcgctg aggcgtattc
    13381 cgccaggcac gacggcaagc tacgcggaga tcgctgcaag catcggtgcg ccggaggccg
    13441 cccgcgcggt tgccggcgct tgcgcggcga acaagatcgc cgtggcgata ccctgccacc
    13501 gcgtcgtggg cgccgacggc acgttgtccg gctaccgttg gggcgtggcg cgtaagcgcg
    13561 cgcttctcgg caaggagaaa tcgggcaact aacgcgcaag gctcttagcg ggtcagcccc
    13621 ttttcggcga gttcctccag atagctgcgg aagcgcggct cctggttctc gccgagatcg
    13681 tgcaagtact cccaaacgta aagcccggtg tcgtgaccat cgtcgaagac gatgcgcacg
    13741 gcgtaattgc ccacaggctc gagacgcgag atgcgcacat tcttcttgcc cggcaccgtg
    13801 atccgttgtt ccgggctgtg gccttgcacc tcggcgcttg ggctcagcac gcgcaggact
    13861 tccgcgggca ggtcgtagct tgctccgtcg tcgaagctca cgatgagggt gcgcttgtcc
    13921 ttgtcgagct tgagctccgt gggccaggcc gtcatttggc cctctcagcc aaggtcgatc
    13981 tcgccttcga ccacgtgatc gagggacgtg cctttggcgg tgagcaccat tctcaccttg
    14041 agcttgccgc cgaggtctcc ctcgcgcaca acccgctcga tcgcctgctg agaggtcacg
    14101 ccgacctcct tcaggaaccg ccgcagtgac atgttgaact gttgctcgtc catcgcttgg
    14161 cctccttctc tgcgccatcc catgatatgg aagaggctac acatctatac tagagacgaa
    14221 accccattat attcagagag tcatggcggt cacttcacaa ggcaaaggcc gcaccaccaa
    14281 gacgcgggcc ggagcgatga aacagaaacg cacggtcaaa agccggaacg cgccagccaa
    14341 ggcgctggcc gacccgcgct atcgcccccg cgtggtcaaa ggcgccaagg tctatagccg
    14401 caaggatcag aaaacaccgg gtgaggacga cggcgcgtag atgagtgaac ttgaagatga
    14461 gcttgccccg cccctgatcg accggttcgg ccgcgccatc acctatcttc gggtgtcggt
    14521 gacggaccgc tgcgacttcc gctgcaccta ttgcatggcg gaggacatga ccttcgtgcc
    14581 gcgaaaagag ctcctggcgc tggaggaaat cgaccggctg gccagcgcat tcatcgcgcg
    14641 cggggtgcgg aaggtgcggc tcaccggcgg cgaacccctg gtgcggcgga acattttgtg
    14701 gctgatcgag gcgctgtcac gccatctcgg cgacggtctc gaggaactca ccctcaccac
    14761 gaatggcagc cagctctacc ggttcgcgga cgcactggcg gatctcggca tcaagcgcat
    14821 caacgtctcg ctggacacgc tggatgcggc gaagttccgc gtgctcacac gctggggtga
    14881 tttcgatcag gtcatgcgcg gcatcgatgc ggcggaccgc gccgggctca agatcaagat
    14941 caacacggtg gcgctgcgcg acatgaatga ggacgagctc gaagatctcc tgcgctgggc
    15001 gcatggccgg ggcttcgacc tcacgctgat cgagaccatg cccttgggcg agatcgacac
    15061 cgatcgcacc gaccaatatc tgccattgtc cttggtgcac tcacggctgt ctcaacatct
    15121 cacgctcacc gacatccccc accgctctgg cggtccggcg cgctacatgc aagtcgcgga
    15181 gaccggcggc cggctcgggc tcatcacgcc catgacccat aatttctgcg aatcctgcaa
    15241 tcgggttcgc gtgacctgca cgggcactct ctatatgtgc ctggggcagg acgacgccgc
    15301 ggacctaagg gcgccattac gggccagcac gtccggcgcg ccgctccacg ccgcgattga
    15361 cgaggcgatg gcgcgcaagc ccaaaggcca tgatttcatc atcgaccggg acgccagacc
    15421 ggccatttcc cggcatatgt cagccacggg cggctaagtc caactacggt taagaaatcc
    15481 ttccggccag caaattcccc aggctttccg cgcttttggc acgcatcccg ctttcttagc
    15541 cgggtacgcg gcgagcaaat ccatccgctt actgcatttt ttaacctccg gccctctaat
    15601 ttggcgccag acatcgtgat ctggcttcag acacgagatt ggccgcggtt cgcacacatg
    15661 ttgttcaaag acctttttaa gaagtactgg cactgggcgg gactggcgct gctttcggcg
    15721 ctgatcgtct cgacggcctt tatcgccccc ggacgcgtga gcctcttgga gctcgagcag
    15781 gaagcgctcg tggcccagga ccgaatccat acgcaaatgc ttcgagaccc gcaggtcgtg
    15841 cacaatgcgc tgaacgttcc gggcgcggcc ccgcagcttg gcgagatctt ccacgcctcc
    15901 ggctacgccc cgcgcgtgct gcgatatgaa gtctacaacg cgaagggacg gcttctcttc
    15961 accagcggcc aagccaatct tcgtctcgac ggtccgcctt tgaacgtgac gaacaacgcc
    16021 acccagcaag ccagcgtcgt cagcctgcag tcgggcgcgg gcaacaatga tccttcgaac
    16081 ttcgcggtgt tgcgcgtgcc catcaacttc gccgcaaatg aacgcggcac gctcatcgtc
    16141 tatctggatc aaagcgacca ggcgagcgtg ctttcaagct acttcagcgt cattgctctt
    16201 gtgactctca ccctgctcgg cgtcggcgtc accatgcccg cggcctttgc atggatgcgc
    16261 agccgcgagc ggcggcaggc cgaagagcag gtgcactatc tccagaacca cgacgcgctg
    16321 acgggcctcg ccaatcgccg cgccttcgcc gagaggcttg gcgacgccac ggcccgcatg
    16381 aagcaggatg acacgcacat cgcggtcatc tgcctggaca tcgacaagtt caaagagatc
    16441 aatgactcga ccgagcatgg cggcggcgac caggtgctgc gcgaaatcgg cgtgcgcatc
    16501 aatgagacct tgcgagaccg cgacatcatc gcacgtctcg gcgccgacga gttcgcgatc
    16561 gcgctggtcg acgtctccaa tctcggcgac gtcatggcct tcatgaaccg gttggtcgcg
    16621 gccttgcagc tgcctttccg tatcgccgac aaggaaatcc tcatcaccac cagcgtcggc
    16681 atcgcgcttg caccggcaga cggcgacacg gcttctgccc tgctacgcca tgcgacgatc
    16741 gccttgtccc gcgccaaggg cgacggtggc cagcgcatgt gcttcttcga ggagagcatg
    16801 gacaaggcgc tccagcgccg gcgcaccgtg gaacacgagc ttcgcctggc cctcggccgc
    16861 gaggagttcg aggtggccta tccgccgcaa ttcgacctgg cgacggaaga gcaatgcggc
    16921 tcggaggctt tgatccgttg gcaccatccg ctgcacggca agatcgcacc gaagcacttt
    16981 atttcggttg ccgaggagac gggactgatt gtgccgatcg gcgagtgggt gctgcgccgc
    17041 gcgtgctgtg acgctgtgag ctggccggag catctgaccg tcgccgtgaa cctgtcgccg
    17101 gcgcagttcc gcgaccgcga catcgccgag acggtcgccg acattctgca cgagacgggg
    17161 ctgccggccg agcgcctcga actggagatc acagagaacc tcctgatcaa cgacaccgag
    17221 gaagtcctgg gcaagctgaa ccgcctgcgc aagatcggtg tgaagatcgc catggacgat
    17281 ttcgggaccg gctattcgag cctgagctat cttgcccgct tcccgttctc caagatcaag
    17341 atcgaccgtc agttcatccg caatatggcg cgcgacccgg cgatgcgcgc gatcgtccgg
    17401 acgatcattg ccctgggcaa atccctcaat gtgacaatca cggctgaagg cgtggaaacg
    17461 cccgaacagg ccaagatctt gagcgagttc ggctgcggtg aagtccaggg cttcttgtat
    17521 ggccgtcccg aggcggcgca accttacgcc aagctggaac agagtgcgga cggcaaggtc
    17581 acccccattc gcgcccgccg ccgcaggcgc ccaagcgcgg catagcttca cgtcgctttc
    17641 tagatctcga ttttcagcgg ttcgctaacg gatattccgc catcggggcg gcctgtctgc
    17701 cgccgagaaa acccttgttg ggggacttcg cctgcaccgg tgcagtgcgc ttttgtgcgt
    17761 taccgcgcga gcgcggcttg acccgcgcct ttcggaccgg cttgcgcgcg acgggcggtg
    17821 cgggcgcgac gaccgtggcg gccgagggtt tgcgcaacgg gaccggcacg atcgcagggg
    17881 acgcttccgg cacctcttgg gaccagatga cctctttgga ctcgatgctg gcaatcgcct
    17941 caccgtcgat gccgccgtcc gcatcgggcg cgggcacgct ttccacgagc ggcagcgcgg
    18001 cgggctcgac agtccgaggc ggcggcgcgg tggcgatgac ctccaaaagc gcggcttgcg
    18061 tttgcagcac atcgggtgat gcaaacgctt gcgacagcga gccagtgatc tccccaacgg
    18121 cggaggccgg catagcgtca aggtcggaac ccggcgaggc accgaggaca gcatcgagga
    18181 cggaggccgg cagggcgtcc tcgtcgatgt cgccctcgcc gatatattcc ggcagcggca
    18241 gcgccgccgt ctcggccatt gccgtctcta acgtcggcgg tggtgtgctc cgcaccacga
    18301 cctggacggc gacagtgcct tgggctttgt cggccggcgc gggcagcgtg atcatgatcg
    18361 gtagcgcgag cgccagcagc gcggcatggg ccgcgccagc accgaaggcg cacatcgaaa
    18421 gacttggttt ctcgtcgtgg agcactgaag gactccgccg ctcacgttcc cccgcgcaac
    18481 gcgtaacgtc cgctccaatc cgagttatct gagtcgcccg gctcccggca agcctctagt
    18541 tttccatgac aattttcggc gccgggcgat cggcgtcttt gtggcgctca agctcggccc
    18601 acgccttcgc ggcgatgccg caataggcct tggcgggttc gccgtccgga tcagtcgcca
    18661 ccacgggctt gcccgcatcc gacgtctcgc gcaccgccat ggtgagcggc acttccccta
    18721 agaacggcac gccgagcttg ttcgcctcct cccgcgcgcc accgtgaccg aaaatgtcgg
    18781 agcgctcacc gcaatgaggg cagaggaagg tcgacatgtt ctccacaatg ccgagcaccg
    18841 gcacgttgac cttcgtgaac atgttcagac ctttgcgcgc gtcgatcagc gccaggtcct
    18901 ggggcgtgga gacaatgacc gcaccggcca gcggcacttg ctgcgccatg gtgagctgcg
    18961 catcgccagt gccgggcggc atgtcgacta cgagcaggtc gagctcgccc caagccacgt
    19021 ccttcagcat ttgcgataag gccgacatga ccatgggccc gcgccagatc atcggcgtgt
    19081 cctcgtcgac gagaaagccc atggacatga tcttcagtcc ataggcacgc atgggatcga
    19141 gcttgttgcc ggcgagttgc tgcggctgac cttgaatgcc aaggagccgc ggcatggacg
    19201 ggccatagat gtcggcgtcg agcacgccga cctcgagccc ccggtctttc agcgccagcg
    19261 caagattgac cgccgtggtc gatttgccga cgccgccttt gcccgacgcc actgcgatga
    19321 tgtgcttcac gcccggcacg ccgccaggac gatggcgcgc atcgcgtgcc gcgcccggcg
    19381 cggtggcgcc cgggccttgc ggtgcttggg gcggcgatgc ttgggccggc gatgattgcg
    19441 gcggtggtgc ttgccgcgcg ccgccattgc tggcgacagg gctgacgctt cccgagcccg
    19501 gctcccggtc ggcggtgagc gtcacggtga caccctccac gcccggcagg tccttcacca
    19561 ccgtctcggc cgcctggcgc aagccctcca gctcgcgcgc gcgcgccgga tcgatggaga
    19621 tggcgaagta gaccttaccc ttatggatca ccacttcgga gacgaggccg agggagacga
    19681 tgtcgtcctt gagatccggc cccttcacgt ctt
//
LOCUS       DBNHVU010000141        13772 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1315906, whole genome shotgun sequence.
ACCESSION   DBNHVU010000141 DBNHVU010000000
VERSION     DBNHVU010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 13772)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13772)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13772
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1315906"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(491..754)
                     /locus_tag="AC16Y7_05655"
     CDS             complement(491..754)
                     /locus_tag="AC16Y7_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708899.1"
                     /translation="MDLTSLLVQVVSGAVGGAGTGAVAKNQSLGTIGNVIAGAVGGGI
                     MGQLIGNFAGQMIEGWVGDIAGGAVGGIILTLIAGFIKNMTAK"
     gene            931..1389
                     /locus_tag="AC16Y7_05660"
     CDS             931..1389
                     /locus_tag="AC16Y7_05660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HIR5708900.1"
                     /translation="MRKSKYTPGGFDLAVRRATAGKGLFAQEPIPKGACIIEYVGPTL
                     TEEEEETSRSRYLFEVTPNKTIDGWNKLNTARYINHSCRPNCEVEIRKGRVWVMARRN
                     IKPGEELAYNYGKNYVESFIKPIGCRCAKCRPALHAANGDRVKAKMKNGA"
     gene            complement(1361..2983)
                     /locus_tag="AC16Y7_05665"
     CDS             complement(1361..2983)
                     /locus_tag="AC16Y7_05665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR5708901.1"
                     /translation="MPAQTFDVVVIGGGHNGLVCAAYLARAGLKVKVLERRSVVGGAA
                     VTEEFHPGFRNSVAAYTVSLLNPKVIADLDLTAHGLRIVERPAMNFLPLPGGEYLLTG
                     EGRTKSEIAKFSQRDANRYDAYGHALTRIADVLRKLVLTAPPNVVRGATLSALRELVH
                     LGTLGNHLRRLDLEEQRLLLDLFTKPAAEFLSDWFESEPIKALLGFDSIVGNYASPFD
                     PGTAYVLLHHAFGEVNGKKGAWGHAIGGMGAITQAMASSAAACGVDIETDAEVAELVI
                     EQGRAVGVALRNGQAIKGRALAANVNLKLLYTQLTSKDALPRAFSERMERFKCCSGTF
                     RMNVALSELPSFTALPGRTAGPHHGAGIIIGPSLAYMDQAYRDARAHGWSRVPIIEML
                     IPSTLDDSLAPSGAHVASLFCQHVAPELGDGRSWDDARDEVADLMIATVDEYAPGFAK
                     SVIARQALTPLDLEREFGLVGGDIFHGALSLNQLFSARPMLGYADYRGPLRGLYHCGA
                     GAHPGGGVTGAPGHNAAKVILADLKRHFSSSL"
     gene            complement(2994..3260)
                     /locus_tag="AC16Y7_05670"
     CDS             complement(2994..3260)
                     /locus_tag="AC16Y7_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708902.1"
                     /translation="MPRPVMFSLKLEKRRKARRFALLIAVTVLILGLGMIAVVAVQGF
                     DLLGAAEADRMPMVLALLGALAGLVFLSLIAYAAARIYGRVTTR"
     gene            complement(3427..3639)
                     /locus_tag="AC16Y7_05675"
     CDS             complement(3427..3639)
                     /locus_tag="AC16Y7_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708903.1"
                     /translation="MKRLLIVLAIVLAPAGLAGCVTEQTPLLPMTLGPSEAAQATCLT
                     YGDTPTFGDCKTVAKTAAPSQATSQE"
     gene            complement(3805..4278)
                     /locus_tag="AC16Y7_05680"
     CDS             complement(3805..4278)
                     /locus_tag="AC16Y7_05680"
                     /inference="COORDINATES: protein motif:HMM:NF019128.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HIR5708904.1"
                     /translation="MADQSVRPRRYNTVSERQLRCLAEGIYFEARSEPLRGQLAVGRV
                     ILNRVQSSSYPDTICDVVYQNDHRHNRCQFSFACDGKPDVIGERQVWSRVRGYAAWLM
                     ANDPEGTEYQVLASLAAATHYHADYVTPHWAKHFELTARIGRHIFYADPSADPSA"
     gene            complement(4702..4824)
                     /locus_tag="AC16Y7_05685"
     CDS             complement(4702..4824)
                     /locus_tag="AC16Y7_05685"
                     /inference="COORDINATES: protein motif:HMM:NF016141.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR5708905.1"
                     /translation="MFPELGLTLTLGGGILGAIIMAFIGAVILLILVKIIKSLT"
     gene            complement(5031..5738)
                     /locus_tag="AC16Y7_05690"
     CDS             complement(5031..5738)
                     /locus_tag="AC16Y7_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIR5708906.1"
                     /translation="MKPVLVIQNDAKEGAGQLATLIAERGLQQDTVLGFDTDYDHLDP
                     ADYSALVVLGGAQSAYETEAYPYLARQMALCQEFMDAGKPIAGFCLGAQILARALGGE
                     VVPGAQKEIGWYDLQLTAEGAHDAILGAHPKTLLAYHFHGDRIESVPGAVNLASSALT
                     EWQLFRHGANVYGFQYHAEVDAPLVEVMCRNNADYMAANGFDAEAIIGASGEHLPAFA
                     KASKEALQRWLDLVAPD"
     gene            complement(5757..6224)
                     /locus_tag="AC16Y7_05695"
     CDS             complement(5757..6224)
                     /locus_tag="AC16Y7_05695"
                     /inference="COORDINATES: protein motif:HMM:NF022114.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HIR5708907.1"
                     /translation="MAHFTTRMMICGAMLGAFGIALVMQAEPARALGDWVEGEESDNC
                     DNDDGAFNEAAFVIATEPEAGERVATGFEVEGCSRTFESNVQWKLLGRDGSELASGNT
                     QGGGVDGPGAFSFTVPYNVNVVQVGHLEVFEEDASGGEGNPPGRTVVPLVLKP"
     gene            6342..6785
                     /locus_tag="AC16Y7_05700"
     CDS             6342..6785
                     /locus_tag="AC16Y7_05700"
                     /inference="COORDINATES: protein motif:HMM:NF016089.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TQO small subunit DoxD"
                     /protein_id="HIR5708908.1"
                     /translation="MTDAHSLERDAPITPACFSLLAIVLLGTRFVQGFIFWSGASRRL
                     FYDFHEIAGVDHAVKLDFEAAGFVANKLVHALPGALWVQAPLEWTLQYPDLILASVWS
                     WTFAELAVGLGLIFGLATRLWAFVSIGLNVALMLIFGWIGLHVPR"
     gene            6793..7434
                     /locus_tag="AC16Y7_05705"
     CDS             6793..7434
                     /locus_tag="AC16Y7_05705"
                     /inference="COORDINATES: protein motif:HMM:NF019300.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TQO small subunit DoxA domain-containing
                     protein"
                     /protein_id="HIR5708909.1"
                     /translation="MAVSGVAMSSAVFLAGGGTIALDRFIDRSGWAARNPWSAWVFSG
                     PLPPGKTRSLGLLLAAIAVVCTVGSYHLLFGAVVSPLHARVNFHRHHIALSETRLSSD
                     GAVTFDAYVDAGPDTGAAYVIAARLIDTAGDTVAAWDGVALAQLPDSAIRNTYPYAWA
                     SHFKTETIGFSGQTDARARITLPPAETAPAGGAASLELEAINGAMWRTELSPK"
     gene            7525..8124
                     /locus_tag="AC16Y7_05710"
     CDS             7525..8124
                     /locus_tag="AC16Y7_05710"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010687241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR5708910.1"
                     /translation="MASPTLRTLTGMDPNPSPISDSALVLIDCQQTYREGVMRLEGVE
                     PALKEAATLLKRFREAGRPVIHIMHDAGVGTPYDITAPIGQIADVVAPIEGETIIVKK
                     FPSSFEQTTLDAELKKLGVKDVVYAGFMTHMCVNSTTRASFNHGYANTVVAGATATRS
                     IPNPATGADVSAAQLQDASLSALGDMFAVVVPHEADVTD"
     gene            complement(8121..8603)
                     /locus_tag="AC16Y7_05715"
     CDS             complement(8121..8603)
                     /locus_tag="AC16Y7_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024106.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpsA SLOG family protein"
                     /protein_id="HIR5708911.1"
                     /translation="MVTVVSGGQTGVDRAALDAGLAAGLPYRGWCPQGGWAEDWPEPP
                     GVFARYPGLKETPARVPEQRTEWNVRDSDALLVLVDGRGLTVSKGTALAVEKAQALGK
                     PHVVIDMDSPDALRQMEDVLRERGAQAICIAGPRESEAPGIYDKAKALLMQTLAANRA
                     "
     gene            8673..9098
                     /locus_tag="AC16Y7_05720"
     CDS             8673..9098
                     /locus_tag="AC16Y7_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708912.1"
                     /translation="MPSIKLLLLGFAAGFLATLIFHQGLWFIFNQTGVIPPSRPAWSM
                     DPIPPLDIPSVLSKAFWGGLWGLVLVLLLGQLEGASYWIAWILVGAVALTAVAFFVVP
                     LLKGQPMQAMGPRFLIGLSVNGAWGFGTALLLRLFSAFR"
     gene            complement(9104..9724)
                     /locus_tag="AC16Y7_05725"
     CDS             complement(9104..9724)
                     /locus_tag="AC16Y7_05725"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006471636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR5708913.1"
                     /translation="MAVLTLYHAAPSRSSIVRWMLEELGEPYDIHLLSLVKGEQQAPD
                     YLAINPMGKVPALRHGDVIVTESAAICTYLADEFPAAKLNAPVGDPRRGPYLRWLFFA
                     AGCIEPATVDHAYPHKEKPRRGALGYGDYDSVMDVTAKAVEAGPYLLGDQFTAADVIV
                     GSIVRWGLQFKLMPDRQEFTDYVGRLEERPALQRTVALDAELAAAS"
     gene            9788..10630
                     /locus_tag="AC16Y7_05730"
     CDS             9788..10630
                     /locus_tag="AC16Y7_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003599719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR5708914.1"
                     /translation="MTAAKTKSASAKEPPAKKDEAEAEAPPLRERLRLLYHGRSLAAH
                     RFRYALLAFDIVTVLFIIATSFMPREPWIEYLDLIFGLVILADFCARIWVSPHPAREF
                     THPATWADMVAIFSFLAPIVGEGLGFLRVLRTLRLLHTYQLVARLRADSPFFRRNEET
                     ITAVINLIVFVFVMTGIVYETQHWRNPAIANYVDALYFTVTALTTTGFGDITLQGSLG
                     RLTAVVIMIFGVTLFLRLLQTLLRPQKVRFPCPKCGLQRHEVDSVCCKACGTRLNIPD
                     EGRF"
     gene            10642..10935
                     /locus_tag="AC16Y7_05735"
     CDS             10642..10935
                     /locus_tag="AC16Y7_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HIR5708915.1"
                     /translation="MASKEPKRWSRNVTEHSHALRLEQGVFILKDPKKIAASLKRSAE
                     ASTRRKADPFRSAMSMLVFYINRAGKNLSEQDRARLEAAKDELRKAFGRPVAK"
     gene            complement(10938..11711)
                     /locus_tag="AC16Y7_05740"
     CDS             complement(10938..11711)
                     /locus_tag="AC16Y7_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708916.1"
                     /translation="MSSVPMSCVDVVWKGNCRDPKVRYRLLSYLHRLAHLSDGYLRQG
                     ERPQLSLVGEGHTPPRPNIEVVDEVLSGPIMISSAIAPHPERLVARAREAGLSVTEPD
                     DGSPHVIMLDEVLLRGLDFKLFDPRDLHPDDNRMSFVFVECPEHHFLDGRLVGVTKKD
                     GEVHLAEPRLRLQTYLEDWTDCLFSWTRFFVVGDFWWQRHEELQGYADYRGVFEELQT
                     TRGSDEAMEATFDAVLSTFTQHAEHGIDEVRSLAKAGKA"
     gene            complement(11945..12463)
                     /locus_tag="AC16Y7_05745"
     CDS             complement(11945..12463)
                     /locus_tag="AC16Y7_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013169198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HIR5708917.1"
                     /translation="MSANTALAISVGVLGAIATWLFLGPLGGMLAIWAAFIAWGCFFH
                     CGGSETGLSSAILGNIAGAIIAGITLWVATQTGIGAKIGLPIWAGLCVGVGVAVMVLL
                     ANIPAFAAIPAQVYGFASVVALTLLGNGAGKLTDVSMDNPVVVIIISMIVGAIFGWVS
                     EKVAGMLAGSGD"
     gene            12705..>13772
                     /gene="trmFO"
                     /locus_tag="AC16Y7_05750"
     CDS             12705..>13772
                     /gene="trmFO"
                     /locus_tag="AC16Y7_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HIR5708918.1"
                     /translation="MTDRVHVIGGGLAGSEAAWQLARAGVAVRLHEMRPQRQTEAHKT
                     ESLAELVCSNSFRSDDYESNAVGLLHEEMRRADSLIMAAADQHKLPAGGALAVDRDDF
                     AGAVTDALLDHPLIEISRAEVEGLPPEGWDSVIVATGPLTSPSLADAVRALTGEAELA
                     FFDAIAPIVHFESIDMDVCWRQSRYDKVGPGGSGADYINCPLDEGQYNAFIDALIAGE
                     KTAFKEFEKTPYFEGCLPIEVMAERGRETLRYGPMKPVGLTNAHNPTVKPYAIVQLRQ
                     DNALGTLWNMVGFQTKLRHGEQTRVFRMIPGLEGAEFARLGGLHRNTFLDSPKLLDQT
                     LRLKADKRLRFAGQVTGVEGYV"
BASE COUNT         2659 a         4375 c         4227 g         2511 t
ORIGIN      
        1 ttcggttgga gcagcttgct cggcaggctg ggggccccgc tgtcggcggc ctgtggcgcc
       61 tcttgggccg cagcacccgc cgtatcgaac aacgccgtcg gcagactcac cgccgcaaag
      121 ccgcaaagaa gcagtgcgca gacccgcaat atacgcgaca ttatttcagt ctccccttac
      181 ccggccgtct ttactagccg gttcggtgcc gctgtaaaag cccctcatcg gccacaaatc
      241 atggccgtcc caacagatct tgggtgcttc cgagtgaaga atgacagcga cgttgtcggc
      301 cgcaccatgg aacggaaaac gtccgacctc gatccagagt gcgcgcgagt agcgcaccct
      361 cggcgagaga tttgcctgac ggaaaggacg atgcaacacg ggaggccatt atcgacaacg
      421 tgatgccgaa gctgtagcgc cacaaaacgt gccggcgcgg caaggtcccg gcctgccgcg
      481 ccgtactact ctattttgcc gtcatattct tgatgaagcc ggcaatcagc gtgaggatga
      541 tgccgccgac ggcaccgccg gcgatatcgc cgacccaacc ctcgatcatc tgtcctgcaa
      601 aattgccgat gagctggccc atgatgccac cgcccacggc gcccgcaatc acgtttccaa
      661 tggtgcccag cgattggttt ttcgctacag cgcccgttcc cgcgccaccg acggcgcccg
      721 agacgacttg aaccaataga cttgtaagat ccatttgatc gttccctccc ttattcgccg
      781 caaccatgcg gcgaggcatt ctaatgctga cacgcatcag gcaccagcct cgttggacat
      841 tgcccctgcg gtcctgtgcg gccttgcaca gggccgcgtt gcgggttacc tgggcccgtt
      901 agcggcgaat cggaaatcaa aagcgaggcc atgagaaaat caaagtacac gcccggcggg
      961 ttcgaccttg ccgtcaggcg cgcaaccgcg ggcaagggcc tgtttgcgca agagcccatc
     1021 ccaaaggggg cctgcatcat cgaatatgtc gggcccacgc tgaccgaaga ggaagaggaa
     1081 acgagccgta gccgctacct gttcgaggtc acgcccaata agacgatcga cggctggaac
     1141 aagctgaaca cggcccgcta catcaaccat tcctgccggc cgaactgtga ggtcgagatc
     1201 cgtaaaggcc gcgtatgggt gatggcgaga cgcaacatca agccgggaga agagctcgcc
     1261 tacaattacg gcaagaacta tgtcgagtcg ttcatcaagc ccattggctg tcgctgcgcc
     1321 aagtgccggc cggcactgca cgccgccaac ggcgaccgcg ttaaagcgaa gatgaaaaat
     1381 ggcgcttgag gtcggcgagg atcaccttcg ccgcgttgtg accgggcgcg ccggtcacgc
     1441 caccaccggg atgagcgccc gcgccgcagt ggtatagccc gcgcaagggt ccgcgatagt
     1501 ccgcatagcc cagcatgggc cgcgcggaga acaactggtt cagcgacagc gcgccgtgga
     1561 agatgtcgcc tccgacgaga ccgaactcac gttccagatc gagaggcgtt agagcctggc
     1621 gcgcgatcac gctcttcgcg aagccgggtg catattcatc caccgtcgca atcatgagat
     1681 cggccacctc atcgcgcgcg tcgtcccaac tgcggccatc gccaagttcc ggtgccacat
     1741 gctgacagaa caggcttgcg acatgggcgc cggacggcgc gagactgtcg tcgagcgtcg
     1801 agggaatgag catttcgata atgggcacgc ggctccagcc atgggcgcgc gcgtcccgat
     1861 aggcttggtc catataggcc aagctcggac cgatgatgat gccggcaccg tgatgggggc
     1921 ccgcagttct tcccggcaac gcggtgaagc tcggcagctc cgacagcgca acattcatgc
     1981 ggaacgtgcc tgagcagcat ttgaagcgtt ccatgcgttc ggagaacgcg cgcggcagcg
     2041 catctttcga cgtcagctgt gtgtagagca gtttgagatt gacgttggcg gcaagggcac
     2101 ggcccttgat cgcttgcccg tttcttaagg caacgcccac ggccctgccc tgctcgatca
     2161 ccagttcggc gacctcggcg tccgtttcga tgtcgacgcc gcacgctgcg gcggagctcg
     2221 ccatggcttg ggtgatcgca cccatcccgc caatggcatg cccccaggcg cctttcttgc
     2281 cgttcacttc gccgaaggcg tggtgcagca ggacgtaggc cgtgcctggg tcgaaggggc
     2341 ttgcgtagtt gccgacgatg gagtcgaagc cgagcagcgc cttgatgggc tcgctctcga
     2401 accagtcgga taagaactcc gccgcaggct tcgtgaacag gtcgagcagg aggcgctgct
     2461 cctccaggtc gaggcggcgt aaatgattgc cgagcgttcc aaggtgaacc agctctcgca
     2521 aggctgaaag cgtcgcccct ctcacgacgt taggcggcgc ggtcagcacc agcttacgaa
     2581 gcacgtcggc gatccgtgtg agcgcgtggc catacgcatc gtagcggttc gcgtcccgtt
     2641 ggctgaactt ggcgatctcg gatttggtgc ggccctcgcc cgtgaggaga tactcgccgc
     2701 cgggaagcgg cagaaagttc atcgccggcc gctcgacgat gcgtagcccg tgggcggtga
     2761 ggtcgagatc ggcaatgact ttggggttga gcagactgac cgtataggcg gccactgagt
     2821 tgcgaaaacc ggggtggaac tcttcagtga cggcggcacc accgacaacg ctgcgccgct
     2881 ccagcacctt caccttgagg ccggcgcggg cgagataggc ggcgcagacg aggccgttgt
     2941 ggcctccccc gatgaccacg acatcgaagg tttgcgcggg caccggagct ttcctagcgc
     3001 gtcgtcacgc ggccatagat ccgcgcggcg gcataggcga tgagggacag gaacacgagg
     3061 cccgccaggg cgccgagcag cgcgaggacc atcggcatgc gatcggcttc cgccgcacca
     3121 agcaggtcga agccttgcac cgccacgacc gcaatcatgc caaggcccag gatgaggacc
     3181 gtgacagcaa tcagaagcgc gaagcgccgg gctttacgcc gcttctcaag cttcaagctg
     3241 aacatgacgg gacgtggcac ggcttccctt ccgcggatcg gtgtacgagg ggcttctccg
     3301 gggtcccgga cacgcctcaa ggcgtggatt tacagcccca tgttcgcccg tgtcgtaccc
     3361 tttggcgggc gcagcggcaa ccgtggcccc cctttgaggg ccatgcggct tcaggggttg
     3421 gcgtctctac tcttgggaag tggcttgcga cggcgcagcc gtcttcgcga ctgtcttgca
     3481 atcgccgaag gtcggggtgt cgccgtaggt caggcacgtt gcctgagcgg cttccgaggg
     3541 ccccaaggtc atgggcagaa gcggtgtctg ctcggtcaca cagccggcga gccccgccgg
     3601 ggccaggaca attgccagga cgatcagaag ccgtttcatg gtcgaaatcc cccggatcgg
     3661 tatccgtgtg aaccaagtct agagataggc gtgaaccagg tctagagata ggctctgaag
     3721 cgccaattac aaggggaaaa ggggcggctg cgcttcaggt cgccgatcgc cacccccttg
     3781 gaaagccctt aggccggaaa gcctttaggc cgatgggtcg gccgacggat cggcatagaa
     3841 gatatgccgg ccaatccggg cggtcagctc gaaatgcttg gcccagtgcg gggtcacata
     3901 gtccgcgtgg tagtgggtcg cggcagccag cgatgcgagg acctgatact cggttccctc
     3961 cggatcgttg gccatcagcc atgcggcata gccgcgaacg cgggaccaga cctggcgctc
     4021 gccgatcacg tccggttttc cgtcgcaggc aaaggaaaac tggcagcgat tgtgccggtg
     4081 atcgttctgg tagaccacgt cacagatcgt gtcgggatag gagctgctct ggacgcggtt
     4141 caagatgacc cggccgacgg ccagctggcc gcgcaggggc tcgctacgcg cctcgaaata
     4201 gatgccttcg gcgaggcagc gaagctggcg ttccgaaacc gtgttgtagc gccgcgggcg
     4261 gacgctctgg tccgccatag cgaacgccgc aagctcaatg cccttggtcg cttcgattac
     4321 cgttgccggc tcctcagaga ctgcagatgg cttactggcc tcatctgcaa gtgccgggac
     4381 caacaacgca agaagaccca aaataagcag gcccgcatgc acgcaaattg acctcaaaac
     4441 ccccataaat tttcctcaac agttgttaac gtttgcgagt cgaacccgcc gagggtgggc
     4501 ttttgctagg aaattctgtc gaaggctgga tcaggttgtg acgaaaccgt ggtaactcgg
     4561 cggaactagt gcgacatatt gttcctgcct caagttgtat cttgaggctt cggacagcat
     4621 ggtgccgccc ggccgcacga cgggtcgccc cgaccccatg cgagtccgca ctaatgctct
     4681 gaagttactg ggtttttcct gctaggtgag gctcttgatg atcttcacca aaatgagcag
     4741 gatcacggcc ccgatgaagg ccatgatgat cgcgccaagg atgccaccac caagggtaag
     4801 cgtgaggccc aactcaggaa acaagaagct cgcgatcagg gcgccgatga tgccgattac
     4861 gatatcgccg atgaggccga atcccgtccc ccgaacgacg atgccagcca gccagccagc
     4921 cagccagcaa tggcgccgac gatcagcgtg ataatgagtg cagtcagcat gtcttatccc
     4981 cctccggttg aagaccgacc ggaaactacc atgagtcggc cccagggcac ctaatccgga
     5041 gcaacgaggt cgagccagcg ttgcagcgcc tctttggagg ccttcgcgaa ggccgggaga
     5101 tgctccccgc ttgccccgat gatcgcttcg gcgtcgaacc cgttggcggc catgtaatcc
     5161 gcattgttgc ggcacatgac ctcgaccaga ggggcatcga cttcggcgtg atactggaag
     5221 ccgtagacat ttgcaccgtg ccggaagagc tgccactccg tcagcgccga cgaggccaga
     5281 ttgacggccc ccggcacgct ctcaatgcga tccccgtgaa aatggtaggc gagcaaggtc
     5341 ttgggatgtg cgccgaggat ggcgtcgtgt gcgccctccg cggtcaactg aagatcgtac
     5401 cagccgatct ccttctgcgc gccgggcacg acctcgccgc cgagcgcccg ggcgaggatc
     5461 tgcgcgccga ggcaaaaccc ggcaatgggc ttgccggcgt ccatgaactc ctgacagagc
     5521 gccatctgac gggcgagata gggatacgcc tccgtctcgt aggcactttg cgcgccgccg
     5581 agcacgacga gcgcgctgta gtccgcaggg tcaagatgat cgtaatcggt gtcgaagcct
     5641 aacaccgtgt cctgctgaag cccacgctcg gcgatcagcg tcgccagctg gccggcgcct
     5701 tccttggcat cattctggat tacgagaaca ggcttcatcc gtgacgtatc tcagccctac
     5761 ggcttgagca caagcgggac gaccgtgcgt cccggcggat tgccttcgcc gcccgaggca
     5821 tcctcttcga acacttcgag atggccgacc tgcacaacgt tcacgttata gggcacggtg
     5881 aaggagaacg cgcccgggcc atcgacgcca ccgccttggg tgttcccgct ggccagctcc
     5941 gacccgtcgc ggccaagaag cttccactgg acgttgctct cgaaggtgcg cgaacacccc
     6001 tcgacttcga aacccgttgc gacacgctca ccggcctcgg gctccgttgc gatgacaaag
     6061 gcggcttcgt tgaacgcccc atcgtcattg tcgcaattgt cgctttcctc gccctcgacc
     6121 caatcaccca acgcgcgggc cggctccgcc tgcatgacga gtgctatccc gaaagcgcca
     6181 agcattgcgc cgcaaatcat catccttgtc gtgaaatgag ccatggcatc ctccctggat
     6241 tgtcaggttg ccaacgcgcc agaccatcac cagcctacgc cgccaacctc aatgctacaa
     6301 tgttcagtgt ccaacccggg ttccatgtca ccgaggtcac gatgacagac gcacactctc
     6361 ttgagcgaga tgctcccatc acgcccgctt gcttttccct cctggctatt gtgctgttgg
     6421 gaacacgctt cgtccaaggg ttcatcttct ggagcggcgc gagccgccgg ctgttctacg
     6481 acttccatga gatcgccggc gtcgatcacg cggtgaagct cgatttcgag gccgcgggtt
     6541 tcgtcgccaa caagctggtt catgccctgc ccggagcgtt gtgggtgcaa gcgcctctcg
     6601 agtggacgct gcaatacccg gatttgatcc tcgcctcagt ttggagttgg acgtttgccg
     6661 agcttgccgt gggccttggc ctgatcttcg gcttggcaac ccggctctgg gcattcgtga
     6721 gcatcggcct caatgtcgcg ttgatgctga tcttcgggtg gattgggctc cacgtgcctc
     6781 gatgagtgga ccatggcggt ctccggcgtc gcgatgagca gcgccgtgtt cctggccggc
     6841 ggcggcacca tcgcgctcga ccgcttcatc gatcgtagcg ggtgggcggc gcgcaacccc
     6901 tggagcgcct gggtattcag cggcccgctg ccaccaggaa aaacaaggtc gctcgggctg
     6961 ttgttggcgg ccatagcagt cgtgtgcacg gtcggcagct atcatctctt gttcggcgcc
     7021 gtggtgtcgc cgctgcacgc acgggtgaac ttccatcgcc accacatcgc cctgtcggag
     7081 acacgcctct catcggatgg cgcggtgacc ttcgacgcct atgtggatgc cggtcccgac
     7141 acgggcgcgg cgtatgtcat cgccgctcgg ctgatcgata ccgcaggcga caccgttgcc
     7201 gcctgggacg gggtggcgct ggcccaactg cccgatagcg ccatacgcaa tacctatccg
     7261 tatgcgtggg cgtcgcactt caagactgag acgatcgggt ttagcgggca gaccgacgcc
     7321 agggcgcgca tcacattgcc tccggcagag acggcaccgg ccggtggcgc ggcgtcgctc
     7381 gagctcgaag cgattaatgg agccatgtgg cgaacggagc tctcaccgaa atagttgtct
     7441 tcgactaatg gaggggccgc tttgtgtccc gtccttggtt agacttccgc cacgcgacgg
     7501 cacgagaccg caaggaggag acgcatggcc agcccgacac ttcgcacgct cacaggtatg
     7561 gacccgaacc catcgccgat ttccgatagc gctttggtgt tgatcgactg tcagcagacc
     7621 tatcgtgagg gcgtcatgcg gctcgagggc gttgagcctg cgctgaaaga ggccgccacc
     7681 cttctcaaac gattccgcga ggctggccgt cccgtgatcc acatcatgca cgatgccggt
     7741 gttgggacgc catacgacat caccgcgccg atcggccaga tcgccgatgt ggtggcaccg
     7801 atcgagggcg agacgatcat cgtcaaaaag ttcccaagct ccttcgaaca aaccacgctc
     7861 gatgccgagc tgaagaagct cggcgtcaag gacgtcgtct atgccggctt catgacgcat
     7921 atgtgcgtga actcgaccac gcgtgcctcg ttcaaccacg gctacgcgaa tacggtcgtt
     7981 gccggcgcca cggcgacacg cagcattccg aaccccgcga cgggcgcgga cgtctccgca
     8041 gcgcagttgc aggatgccag cctctcggcg ctcggcgaca tgttcgccgt ggtcgtgccg
     8101 cacgaggccg acgtcacgga ctaagctctg ttggccgcga gcgtctgcat gaggagagcc
     8161 ttcgccttgt cgtagatgcc gggggcttcg ctctcgcgcg gaccggcaat gcaaatcgcc
     8221 tgtgcgcccc gctctctcaa gacgtcttcc atttgccgca acgcatccgg gctatccatg
     8281 tcgatgacaa catgcggctt gccgagcgct tgcgctttct caacggcaag cgcggtgcct
     8341 ttggagacgg taagcccacg tccatcgaca agcaccagca aggcatcgct gtcgcgaacg
     8401 ttccattcgg tgcgctgttc cggcacccgc gccggggtct ccttgagacc ggggtagcgc
     8461 gcaaagactc cgggcggctc gggccaatcc tccgcccagc cgccttgggg acaccaacca
     8521 cgatatggga ggcccgcggc aaggcctgcg tccagagccg cgcggtccac gcccgtctgc
     8581 ccgccagaga cgaccgtcac catatcgtgt cctcgtgaag cgttgcgctc aatgccggaa
     8641 ggcgcatcat gggcgaaaag ggggagaccg tcatgccatc catcaaactg ctgctccttg
     8701 gcttcgccgc cgggttcctc gccacgctga tcttccatca ggggctttgg ttcatcttca
     8761 accagacagg cgtcatcccc ccatcgcgtc ccgcctggtc aatggacccg atcccgcccc
     8821 tcgatattcc ttcggttctg tcgaaagcct tttggggcgg tctttggggc ctcgtgcttg
     8881 tgctcctcct tggacagctc gaaggcgcca gctattggat cgcctggatc cttgtcggcg
     8941 ccgtggcgct cacagccgtc gcctttttcg tcgtgcccct cctgaagggg caaccgatgc
     9001 aggcgatggg tccgcgcttt ctgatcgggc tttcggtgaa cggcgcctgg ggcttcggca
     9061 cggctctact gctacgcctg ttcagtgcgt tccgctaagg ggctcaagat gccgccgcga
     9121 gctccgcatc gagcgcgacg gtacgctgga gcgcaggcct ctcctcaaga cggccgacgt
     9181 agtccgtgaa ctcctgccgg tcgggcatga gcttgaactg caagccccag cgaacgatgg
     9241 agccgacaat cacgtcggcg gcggtgaact gatcgcccaa gagataaggc ccagcctcga
     9301 ccgccttggc cgtgacgtcc atgacggagt cgtagtcgcc atagccgagt gccccacgcc
     9361 gcggtttctc cttgtgcgga taggcatgat ccaccgtcgc aggctcgatg cagcctgccg
     9421 cgaagaacaa ccatctgaga tagggtccgc ggcgcggatc gccgacaggc gcgttcagtt
     9481 tggcagcggg gaattcgtcg gcaagatagg tgcagatcgc cgccgactcg gtgacaatga
     9541 catcgccatg gcgcaacgcg ggcaccttgc ccatggggtt gatcgccagg tagtcgggcg
     9601 cctgttgctc acctttgacc aaactcaaca ggtggatatc gtaaggctcg ccgagctcct
     9661 ccagcatcca gcgtacgatg gatgagcgcg acggcgcggc gtggtaaagc gtaagaactg
     9721 ccattttcat ggtctccact gatacggcgg cacgatagcg caacgccgcc acctaaagaa
     9781 gacgctcatg accgccgcaa agaccaaatc cgcctcagcc aaagagcctc ccgccaagaa
     9841 agacgaggct gaggccgaag ctccgccgct tcgcgagcgg ttgcggctgc tctatcacgg
     9901 ccgcagcctg gcggcacacc gcttccgcta cgcgcttctg gccttcgaca tcgtcacggt
     9961 tctgttcatc atcgccacgt cgttcatgcc gcgcgagccg tggatcgagt atctcgatct
    10021 catcttcggc ttggtgatcc tcgccgattt ctgtgcccgc atctgggtga gcccgcatcc
    10081 cgcgcgtgaa ttcacacatc cggccacttg ggcggacatg gtggcgatct tctcgttcct
    10141 cgcgccgatc gtcggcgaag ggcttggctt cctgcgtgtc ttgcggacgt tgcggctgct
    10201 gcacacctat caactcgtcg cgcgtttgcg cgccgacagc ccgttcttcc ggcggaatga
    10261 agagaccatc accgcggtga tcaacctcat cgtgtttgtc ttcgtcatga cggggatcgt
    10321 ctacgagacg cagcattggc ggaaccctgc catcgccaac tatgtcgatg cgctctattt
    10381 caccgtgacg gcgctcacca ccacgggctt tggcgacatc accttgcagg gttcgctggg
    10441 gcggctcacc gccgtggtca ttatgatttt cggcgtgacc ttgttcttgc ggctgctgca
    10501 aaccttgttg cggccgcaaa aggtgcgctt cccctgcccc aaatgtggat tgcagcggca
    10561 cgaggtggac tcggtttgct gcaaggcgtg cgggacccgg ctcaacattc ccgatgaagg
    10621 ccgcttctag cgaggcctgc catggcatcg aaggagccga aacgctggtc tcggaacgtg
    10681 acggagcact cgcacgcgct ccgtctggag caaggcgtct ttatcctcaa agacccgaag
    10741 aagatcgccg catcgctgaa acgctccgcc gaggcgagca cgcgccgtaa ggccgacccc
    10801 ttccgctcag ccatgtctat gctggtgttc tacatcaatc gcgctggcaa gaacctgtcg
    10861 gaacaggacc gggcgcgcct ggaggcggcg aaggacgagc tccgcaaagc attcggacgg
    10921 ccagtggcga agtgatccta ggctttccca gccttagcaa ggctcctcac ctcgtcgatc
    10981 ccatgttcgg cgtgctgcgt gaacgtggag agcaccgcgt cgaaagttgc ctccatcgct
    11041 tcgtcactgc cgcgcgtggt ctgcagctcc tcgaacacgc cgcgatagtc cgcgtagcct
    11101 tgcagctctt cgtggcgctg ccaccaaaag tcgccgacga caaagaaccg ggtccaggaa
    11161 aacaaacaat cggtccaatc ctccagatag gtctggaggc gaaggcgcgg ttcggcgaga
    11221 tgaacctcgc cgtccttttt ggtgacccct accagccggc catcgaggaa gtgatgctcc
    11281 ggacactcca cgaagacgaa gctcatgcgg ttgtcatccg gatggagatc gcggggatcg
    11341 aacagcttga agtcgaggcc gcgcaggaga acctcgtcga gcatgatgac atgcgggctc
    11401 ccatcgtccg gctcggtcac ggagagcccg gcctcgcgcg cacgggccac cagcctttcg
    11461 ggatgaggcg cgatggcgct tgagatcata atcgggccgg agagtacctc gtcgacgact
    11521 tcgatattgg gccgcggcgg ggtgtgtccc tcacctacga ggctcagctg aggacgttcg
    11581 ccctggcgca gatacccgtc gctcaaatga gccaggcgat gcaaatagct cagcagccga
    11641 taccggactt tcggatctcg gcagtttcct ttccagacga catcgacgca ggacatgggg
    11701 acactagaca tgggcaagta caaggtgctc cgctgacacg agatgagaag gcgcaaatgg
    11761 cccccaaaga gggccgcaca ttgccgccgc aactcgctgc gcgcaagcag gtccggaaaa
    11821 caaggttgct cagagcttca ccggaaagaa taaacggcgt ggcctcaact ggaggccacg
    11881 ccgccttcgg ccatgcaggt cccgggccta gggggccccg gggcgcgata gccgcattgc
    11941 cagtttagtc tcctgagcca gccagcatgc cggcgacctt ctcagaaacc catccgaaga
    12001 tcgcacccac gatcatcgaa atgatgatga caaccacagg attgtccatg gagacgtcgg
    12061 tcagtttgcc ggcgccgtta ccaagcagcg tcagggccac cacagaggcg aaaccgtaaa
    12121 cctgagcggg aatggcggcg aaagccggaa tattggccag cagcaccatc acggcaacgc
    12181 ctacgccgac gcacaacccg gcccaaatcg gcagaccgat cttggcgccg atccccgtct
    12241 gggtcgccac ccagagcgtg ataccggcga tgatcgcccc ggcgatattg ccgaggatag
    12301 ccgacgacag gccagtttct gagccaccgc aatggaagaa gcatccccaa gcgataaagg
    12361 ccgcccaaat cgccaacatg ccaccaagcg gccctaagaa gagccacgtg gcaatggcgc
    12421 caagaacacc aacactaatt gcaagagctg tattcgcgga catttcaccc cctttttttg
    12481 cgaaaagaga gcgggcggat ccgccgattg ccggaaatcc accggctcat tccacggaag
    12541 cctgtgtttg catttcgatt cggcttccgg ttgcgactat agcccaaagt ggtgcccgtc
    12601 tgaacaagga ttggtctttt gcccaagaaa tagacactcc tccggcgtgt ccctcgaggg
    12661 ggttctgctt gaaactactg gccgcgcggg cctagttatg caggatgacg gacagggttc
    12721 atgtgattgg cggcggactg gcgggcagcg aggccgcttg gcagctggcg cgcgcgggcg
    12781 tggccgtgag gctgcacgag atgcgccccc agcggcagac cgaggcgcac aagacggagt
    12841 ccctcgccga actcgtgtgc tccaattcgt tccgctcaga cgattacgag agtaatgccg
    12901 tcgggctcct gcatgaggag atgcgccggg cagactcgct catcatggcg gcagccgatc
    12961 agcacaaatt accagccggc ggcgcgctcg cagtcgaccg ggacgacttt gccggtgcgg
    13021 tcacggacgc gcttctcgac catcctctga tcgagatcag ccgcgcggag gtcgaaggcc
    13081 tgccgccgga aggctgggac agcgtcatcg tggccacggg gcccctcacc tcgccgtcgc
    13141 ttgctgacgc cgtccgcgcg ctcacgggcg aagcggaact cgccttcttc gacgcgatcg
    13201 cgccgatcgt tcattttgag tccatcgaca tggacgtttg ctggcgtcag tcgcgctacg
    13261 acaaggtggg cccgggcggt agcggcgccg actacatcaa ctgcccgctc gacgaaggcc
    13321 aatacaacgc tttcatcgat gcgctgatcg ccggcgagaa gacggcgttc aaggagtttg
    13381 agaagacgcc ttacttcgaa ggctgcctac cgatcgaggt catggcggaa cgcgggcgcg
    13441 agacgctgcg ctatggtccg atgaaaccgg tgggtttgac gaacgcgcac aatccgaccg
    13501 tgaagcctta cgccatcgtg caactgcgcc aggacaatgc gctcggcacg ttgtggaaca
    13561 tggttgggtt ccaaaccaag ctccgccacg gcgagcagac gcgcgtattc cgcatgattc
    13621 cgggattgga aggcgccgag ttcgcgcgcc tcggcgggct gcatcgcaac accttcctcg
    13681 acagtccgaa gctgctcgat caaaccttgc gcctaaaggc ggacaaacgg ctgcgcttcg
    13741 ccggacaggt gacgggtgtg gaaggctatg tc
//
LOCUS       DBNHVU010000142         6524 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_350046, whole genome shotgun sequence.
ACCESSION   DBNHVU010000142 DBNHVU010000000
VERSION     DBNHVU010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_350046"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="AC16Y7_05755"
     CDS             <1..405
                     /locus_tag="AC16Y7_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708919.1"
                     /translation="MLSKIVSAAQTGNLEAMRTALEANELKPMVAAAHVSDPIQHWKE
                     QSATGDGRDVLAAMLNIIASGGVLTGKGHDAMYVWPYFAEADIAELTPPQQVEFHRVV
                     PPKAAAAILKSGKYDYYRLGISPAGVWHYFIR"
     gene            complement(395..1738)
                     /locus_tag="AC16Y7_05760"
     CDS             complement(395..1738)
                     /locus_tag="AC16Y7_05760"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR5708920.1"
                     /translation="MAETFDIVFKGGTVVNQDGEGLADIGVRDGKIRAIGDIGEGLAG
                     ETIDARGLHILPGVIDSQVHFREPGMEHKEDLETGSRSAVLGGVTAVFEMPNTKPPTT
                     SAEALEAKVDAATDRMHCDFAFFIGATRENIDELAELERLPGAAGVKVFAGSSTGDLL
                     VDDEESLARMFATIRRRASFHSECESRLKERVSVQREGDPASHTEWRDPIAAIASTER
                     LLRLATDAGKRIHILHISTGAEMELLSLHKDVASVEVTPQHLTLATPDAYERLGTRAQ
                     MNPPLRDQANQDILWWGLQQGVVDVLGSDHAPHTLDEKAGSYPATPSGMPGVQTLVPV
                     MLDHVNAGRLSLARFVDLTSAGPQRLFGIAGKGRIAAGYDADLTVVDLKAERVIENSW
                     IGSKCGWTPFAGRTVKGWPVGTIVRGRLAMWDGELGPAGGAAVRFGEALPRAANV"
     gene            1816..2667
                     /gene="ygfZ"
                     /locus_tag="AC16Y7_05765"
     CDS             1816..2667
                     /gene="ygfZ"
                     /locus_tag="AC16Y7_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAF17-like 4Fe-4S cluster assembly/insertion
                     protein YgfZ"
                     /protein_id="HIR5708921.1"
                     /translation="MSHCQAALLLDRGALRVTGADAAKFLQGIITSDLDKTEDGGAVH
                     AALLTPQGKILFDFFVVADDGGFLIEAAKDTLPELSTRLGFYRLRAAVEIAEVPDLQV
                     AAVWDGTADLPEGATSFADPRLAALGTRILLPKGSTIGGCDESGAGAYHAHRIRLGVP
                     EGGRDFALGDSFPHEALFDQLNGVDFEKGCFVGQEVVSRMQHRGTTRKRVVPVEGDAP
                     LESGTEVRAGDLPIGRIGSVDGQFGLALLRLDRAADASAKGTKLTAGETAITLHKPAF
                     AQFDVPA"
     gene            2664..3245
                     /locus_tag="AC16Y7_05770"
     CDS             2664..3245
                     /locus_tag="AC16Y7_05770"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR5708922.1"
                     /translation="MTDAAQKNCPWPGTDPLYVAYHDEEWGVPERDDRALYEKLILDG
                     FQAGLAWITILRKREAFRRAFDDFAPEKIARYNAKKVETLMQDASIVRNRMKIEGTIA
                     SARVWLEMMETGPGFSAFLWDFVDGKPIVNRYEAMDQVPAETALSRAISKELKSRGFK
                     FCGPTIVYAFMQATGMVNDHLTTCHRHAACTAL"
     gene            complement(3252..3491)
                     /locus_tag="AC16Y7_05775"
     CDS             complement(3252..3491)
                     /locus_tag="AC16Y7_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008125023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HIR5708923.1"
                     /translation="MAKLPRFSLSYNNKNRKWELKREGSGEVVRRFKTKAAAIKGGVL
                     ERAVKKLGSVRIRKRDGKIQEERTYPRSMDPRGRG"
     gene            3657..4271
                     /locus_tag="AC16Y7_05780"
     CDS             3657..4271
                     /locus_tag="AC16Y7_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR5708924.1"
                     /translation="MNNVSGKTAPRAWQRMLSGRRLDLLDPSPLDIEIEDIAHGLARV
                     ARWNGQTSGDYTFSVAEHSLLVEEIAGDIDGSLSREYRLAALLHDAPEYVIGDLISPF
                     KAALSLDYHAFEARLLGAIHVRFGLPPALPDATTKLIKRADKAAAYFEATGLAGFSLD
                     EARRFFGAPKGLSPKLVTWLTQLSPLPAAEGQARFLKRYHELAR"
     gene            4354..4881
                     /locus_tag="AC16Y7_05785"
     CDS             4354..4881
                     /locus_tag="AC16Y7_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009758786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phosphatase family protein"
                     /protein_id="HIR5708925.1"
                     /translation="MTPDTIFVSPLSLVETTVVDAQISHLVTLINGETLIDTPPTIGR
                     KQHLRLAMNDISAPQDGLVVPTETHVAELIQFARDWDQRAPLLIHCWAGISRSTAGAF
                     ISLCALNPETDEHALARALRRASPTAYPNRRLVALADAVLERSGRMNAAVEHIGRGQL
                     AEEGKVFSLPARHAA"
     gene            4885..5895
                     /locus_tag="AC16Y7_05790"
     CDS             4885..5895
                     /locus_tag="AC16Y7_05790"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5708926.1"
                     /translation="MANPRGSDVTAADASISDPHIELGLNAAIVMVRNQQPHILVARP
                     GAAGPGTGDALPFGPFAPRAHRTLEIGLREWVKRQTGIELGYAEQLYTFGDRGRHAEP
                     GGAHTLSVGYLALTRDAGEEKMGAHWSPWYTYFPWEDWRAGKPEILTEEIEPHLKEWA
                     ERPPPPNAPARPLRRPERLKIGFGIGGGWDEEKVLERYELLYEAGLVEEAKRDGRSAA
                     NQWADLPEIGRPMALDHRRILATAMGRVRGKLKYRPVVFELLPAEFTLYDLQKTVEAI
                     SGSLLHKQNFRRLVERGGLVEPTGNVSTQTGGRPARLYRFRREVILERPAPGLRVKAT
                     RT"
     gene            5933..>6524
                     /locus_tag="AC16Y7_05795"
     CDS             5933..>6524
                     /locus_tag="AC16Y7_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR5708927.1"
                     /translation="MVMVGRFSQVFHAFGRLGALGLATAVVSLSLLPAACVAENRTIE
                     TETGKIKVETLASGLDHPWGMTFLPDGRLLVTERAGDLRLLSPDGTLSEPLAGVPEVV
                     AKGQGGLLDVALDPGFAENGLVYLTYAEAGDDGAGTALARGRLTDSGLEDVKVIFRQM
                     PKVTGGRHFGGRLEFAPDGTLFVTLGDRGNDDLVQDL"
BASE COUNT         1171 a         2101 c         2067 g         1185 t
ORIGIN      
        1 atgctgagca agatcgtttc cgccgcgcag accggaaatc tcgaggcgat gcgcacggcg
       61 ctcgaagcga atgagctgaa gcccatggtg gccgcggccc atgtgagcga tccgatccaa
      121 cactggaagg agcagtcggc caccggcgac ggccgcgacg ttctcgccgc catgctcaac
      181 atcattgctt ccggcggagt actgacggga aaagggcacg acgcaatgta tgtctggcct
      241 tacttcgccg aagccgacat cgccgaactc acgccgccac aacaggtgga gttccaccgc
      301 gtcgtgccgc ccaaggccgc ggcggccata ttgaagagcg gcaaatacga ttattaccgg
      361 ctcggcatct cgccagcggg cgtgtggcac tacttcatac gttagcggct ctgggcaatg
      421 cctccccgaa acggaccgcc gccccgccag ccggccccag ctcgccgtcc cacatggcaa
      481 gccgtccccg cacaatcgtg cccacgggcc agccctttac ggtgcggccc gcaaagggcg
      541 tccagccgca tttgcttccg atccaagagt tctcgatgac gcgctccgcc ttgagatcga
      601 cgacggtcaa atccgcatcg taaccggcgg cgatgcggcc cttgccggcg atcccgaaaa
      661 ggcgctgcgg accggcgctg gtcagatcga caaagcgcgc aaggctcaaa cgcccggcat
      721 tcacgtgatc gagcatgacg ggcacgagcg tttgtacgcc cggcatgccg gacggtgtcg
      781 ccggatagct gccggctttt tcatccagcg tgtgcggcgc gtggtccgac ccaagaacgt
      841 cgacgacgcc ctgctgcaat ccccaccaca agatgtcctg attggcctga tcgcgcagcg
      901 gcgggttcat ttgtgcgcgc gtgcccagcc tctcataggc atcaggcgtt gccagggtga
      961 gatgttgcgg cgtgacctca acgctggcca cgtccttgtg gagcgacagg agctccatct
     1021 ccgcgcccgt cgagatatgc aggatatgga tgcgtttgcc ggcgtccgtg gcgagccgca
     1081 acaggcgttc ggtggaggcg atcgccgcaa tcggatcgcg ccattcagtg tgacttgcgg
     1141 gatcgccttc gcgctgcacc gagacgcgct ctttcagccg cgactcgcac tcggagtgga
     1201 acgacgcgcg ccgccggatc gtggcgaaca tgcgggctag gctctcctcg tcgtccacga
     1261 gcaaatcgcc ggtggacgag ccggcgaaaa ctttgacgcc ggcggcgccg ggcagacgct
     1321 cgagctcggc gagctcgtcg atgttctcgc gggtggcgcc gataaaaaag gcgaagtcgc
     1381 aatgcatgcg gtccgtcgcc gcgtcgactt tcgcctccag ggcttccgcg ctcgtggtgg
     1441 gcggcttggt gttgggcatc tcgaacaccg ccgtcacgcc gccgagcacc gccgagcggc
     1501 tgccggtctc cagatcctcc ttgtgctcca tgccgggctc gcggaaatga acctggctat
     1561 cgatcacccc tggaagaata tgcagaccgc gtgcgtcgat ggtctcgccg gcaagccctt
     1621 ccccgatatc gccgatcgcg cgaatcttgc cgtcgcgaac gccaatatcg gctagcccct
     1681 cgccgtcctg attgacgacg gttccgccct tgaacacaat gtcgaacgtc tcggccatgg
     1741 gtgcggtccg gtctttctaa gagtcagcgc ctatgctcac acgattcccg cattttcgga
     1801 acagaagggc gctccatgag ccattgccaa gcagcgcttc ttttggatcg cggggctttg
     1861 cgcgtgactg gcgcggacgc ggccaagttc ctgcaaggga tcatcaccag cgacctcgac
     1921 aagacagagg acggcggagc cgtccatgcg gccctattga ccccgcaagg caagattctg
     1981 ttcgactttt tcgtggtggc ggacgacggc ggctttctca tcgaggcggc aaaggacacg
     2041 cttcctgagt tgtcgacgcg gctgggcttc tatcgcttgc gagctgcggt cgaaatcgcg
     2101 gaggtgccgg accttcaggt tgctgctgtc tgggacggca cggccgattt gccagagggc
     2161 gcgacctcct tcgccgaccc acgccttgcg gcgctcggca cacgcatttt attgccgaag
     2221 ggaagtacga ttggcgggtg cgatgagagc ggcgcaggag cctaccacgc gcaccgcatc
     2281 cggctcggcg taccggaggg cggacgcgac ttcgcacttg gcgatagctt cccgcatgag
     2341 gccctgttcg atcagctgaa cggggtcgat ttcgagaagg gctgctttgt tggccaggag
     2401 gtcgtatcgc gcatgcaaca tcgtggcacg acccgcaagc gcgtggtccc cgtggagggc
     2461 gatgcgcctc ttgagtcggg caccgaggtg cgcgccggcg acttgccgat tggcaggatc
     2521 ggttcggtgg atgggcagtt tggactggcg ctgcttcgcc tcgaccgcgc ggccgacgca
     2581 tcggccaaag gcacgaagct tacggcgggc gaaactgcaa ttacgctgca caaacctgca
     2641 ttcgcgcagt tcgacgtacc cgcatgacgg acgcggcgca aaagaactgc ccgtggccgg
     2701 gcacagaccc gctctacgtg gcttaccacg acgaggaatg gggcgtgcct gaacgcgacg
     2761 accgcgcgct ctacgaaaag ctcatcctcg acggctttca ggccggactc gcttggatca
     2821 ccatcttgcg caagcgcgag gccttccgcc gtgccttcga cgacttcgcg cccgagaaga
     2881 tcgcgcgcta caacgccaag aaggtcgaga ccctgatgca ggacgcgagc atcgtgcgca
     2941 accgcatgaa gatcgagggg acgattgcgt cggcgcgcgt gtggcttgag atgatggaga
     3001 cgggcccggg cttctcggcc ttcttgtggg acttcgtcga cggcaagccg atcgtcaacc
     3061 gctacgaagc catggaccaa gtgcccgcgg agacggcgct gtcacgcgcc atctccaagg
     3121 agcttaagtc tcggggcttt aagttctgcg ggccgacgat cgtctatgcc ttcatgcagg
     3181 ccacggggat ggtgaatgat cacctgacga cctgtcaccg ccacgcggcg tgcaccgcgc
     3241 tttaagtgat gtcagccgcg gccgcgtggg tccatgctgc gtggataggt gcgctcttct
     3301 tgaatcttgc cgtcgcgctt gcgaatgcga accgagccta acttcttgac ggctctttcc
     3361 agcacgcccc ccttgatcgc ggcggctttg gtcttgaacc gcctaaccac ttcaccggat
     3421 ccctcgcgct tcagctccca ctttctattc ttattgttgt agctgagcga aaacctgggc
     3481 agtttcgcca tgatggcctc ccaactctct atttcacttg tcactctctc caatgagccg
     3541 gttatgaatg acattcagga cgcagatcgt ctaccgtttt tctcaatgac gccatttgac
     3601 tcatttgcgc gaactcgctc agtgtctcgg cgagtcatga tgagtactga aagcagatga
     3661 acaacgtgtc cgggaagact gcgccacgtg cttggcaacg catgctgagc ggacgccggc
     3721 tcgacctgct cgatccctct ccgctcgaca ttgagatcga ggacattgcg catggcttgg
     3781 cgcgagtggc acgctggaac ggacaaacgt ccggcgacta cacattctcc gtggcggagc
     3841 acagcctgct cgtggaggag atcgccggag acatcgatgg atcgctctca cgggagtacc
     3901 ggctcgcggc cctgctccat gacgcccccg aatatgtgat tggcgacttg atcagcccgt
     3961 tcaaggcggc gctgtccctg gactaccacg catttgaagc aaggctgctc ggtgccatcc
     4021 atgtgcgctt cggcctaccg ccggcgcttc ccgacgccac caccaagctc atcaagcgcg
     4081 ccgacaaagc cgccgcctat ttcgaagcca ccggccttgc cgggttctcc ctcgacgagg
     4141 ccaggcggtt ctttggggcg ccgaagggcc tcagtcccaa acttgtgacc tggctgaccc
     4201 agttaagccc acttcccgcg gcagaagggc aggcaaggtt cttgaaacgg tatcatgagc
     4261 ttgccagatg aaggctgtgg ggacttgcat gcgattcgat aggttcaaat ggacacgctg
     4321 atttctgaga aagatcgaac taaggtgccg gccatgacgc cggataccat cttcgtttcg
     4381 ccgctgagcc tggtcgagac cacggtcgtg gacgcccaga tcagccatct ggtgacgctc
     4441 atcaacggcg agaccctcat cgacacgccc cccaccatcg gcaggaagca gcatttgagg
     4501 ctcgccatga acgacatcag cgcgccgcaa gacgggctgg tggtgccaac ggagacccat
     4561 gtggccgagc tcatccagtt cgcccgcgac tgggaccaac gcgcgcctct cctgatccat
     4621 tgctgggccg gcatcagccg ctcgacggcg ggtgccttta tatctctatg tgcgctgaac
     4681 cctgagacgg acgagcatgc gcttgcccgg gcgctgcgcc gcgcttcgcc caccgcttat
     4741 cccaaccgcc gtctcgtggc tcttgcagac gccgtgcttg agcgcagcgg ccgcatgaac
     4801 gcggcggtcg agcatatcgg ccgcggccaa ctcgccgagg aaggaaaagt gttttcgctt
     4861 cccgcgcggc acgccgcata agccatggcc aacccccggg gcagtgacgt aaccgccgcg
     4921 gacgcctcca tcagcgaccc tcacatcgaa ctcggcctga acgcggccat cgtcatggtc
     4981 cgcaatcagc agccgcacat cctcgtggcg cgtccgggcg cggcgggacc cggcacgggc
     5041 gatgcgctgc ccttcgggcc gttcgcgccg cgcgcgcatc gcacgctcga gatcggcttg
     5101 cgcgaatggg tgaagcggca aacaggcatc gagctcggct acgcggagca gctctatacg
     5161 ttcggcgacc gcgggcggca cgcagagccc ggcggcgcgc acacactctc ggtcggctat
     5221 ctcgcactga cgcgcgacgc cggcgaagag aagatgggcg cacattggtc gccttggtac
     5281 acctactttc cttgggagga ctggcgggcc gggaaaccgg agatcctgac ggaggaaatt
     5341 gaaccgcacc tcaaagagtg ggcggagcgc ccgccgccac cgaacgcacc cgcgcggccg
     5401 ttacggcgtc ccgagcgttt gaagatcggc tttggcattg gcggcggctg ggacgaggag
     5461 aaggtgctgg agcgctacga gcttctctac gaggccggcc ttgtcgagga ggccaagcgc
     5521 gacggccgct cggcggccaa tcaatgggca gacctgcccg agatcgggcg gcccatggcg
     5581 ctcgatcacc ggcgcattct cgctaccgcc atggggcgcg tgcgcggcaa gctgaaatac
     5641 cgtcccgtgg tctttgagtt gctgccggcc gagttcacgc tttacgatct gcagaagacc
     5701 gtcgaggcca tctctgggag cttgctgcat aagcagaact tccggcggct ggtggagagg
     5761 ggcggactgg ttgagcccac cggcaatgtc tccacgcaga ccggcgggcg cccggcccgg
     5821 ctctacaggt tccgccggga agtgatcttg gagcgcccgg caccgggctt gcgcgtgaaa
     5881 gctacccgca cctaacgggc cttgaagacc aagagcgaag gaacgacatg gaatggttat
     5941 ggtcgggcgg ttctctcagg tctttcacgc gtttgggcga ttgggcgcgc ttggcttagc
     6001 cacggcggtc gtatcgctat cgctgcttcc tgcggcctgc gtcgccgaga atcggaccat
     6061 cgagaccgag accggcaaga tcaaggtcga gacgctggcc agcgggctcg atcatccctg
     6121 gggcatgacc ttcctgcccg atggacgcct gctggtgaca gagcgggccg gcgatttgcg
     6181 gctcctgtcg ccagacggga ccctgtcgga accgctcgcg ggcgtgccgg aagtcgtggc
     6241 caagggccaa ggcggccttc tggacgtggc gctcgacccg ggcttcgcgg agaacggtct
     6301 cgtctatctc acttacgcgg aagccggcga tgacggcgcc gggaccgccc tggcccgtgg
     6361 gcgcctcacc gatagcggtc ttgaggatgt gaaggtcatc ttccgccaga tgccaaaagt
     6421 caccggcggc aggcatttcg gcggacggct tgagtttgcg ccggacggca cgctcttcgt
     6481 gacccttggc gaccgcggca acgacgatct tgtccaagat cttt
//
LOCUS       DBNHVU010000143         3572 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1391141, whole genome shotgun sequence.
ACCESSION   DBNHVU010000143 DBNHVU010000000
VERSION     DBNHVU010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1391141"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1345
                     /locus_tag="AC16Y7_05800"
     CDS             2..1345
                     /locus_tag="AC16Y7_05800"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HIR5708928.1"
                     /translation="MLALRGLSSPETSRQGNYMGMAGMAIAVATTLAVAQPESALSWA
                     MIVGGIAVGGAIGAVIARRIAMTAMPELVAAFHSLVGMAAVLVAAAAYYAPEAFGIGA
                     TGAIRVASLIEMSLGAAIGAITFTGSVIAFAKLSGRMSGAPILLPARHLINITLVIGL
                     VACIVLFCQEQTPAYFWAIVGLSLLLGVLIIIPIGGADMPVVISMLNSYSGWAAAGIG
                     FTLGNTALIVTGALVGSSGAILSYIMCKGMNRSFISVILGGFGGEMAGPAGDGEQKPV
                     KLGSAEDAAYLMKNASRVIIVPGYGMAVAQAQHALREMGDGLKEAGVEVKYAIHPVAG
                     RMPGHMNVLLAEANVPYDEVFELEDINSEFPQTDVVYVIGANDVVNPAAEDDPSSPIY
                     GMPVLQVWKSGTVLVNKRSLASGYAGIDNPVFYGDNTMMVLGDAKKMTEDIAKAL"
     gene            1711..2583
                     /locus_tag="AC16Y7_05805"
     CDS             1711..2583
                     /locus_tag="AC16Y7_05805"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5708929.1"
                     /translation="MLNLFLSFAGIFALICLGGRLLHRYFVYIPDPRRIAPKAAGLVS
                     VDEIVFKAADGTRLIAWYREAQPGKPTLLYFTGNSGNTADRASKIKAIGADGYGVFML
                     NYRRCGGSGGNPTEKRITADAVSAYDCLRGLGVAPRDIVVYGESLGTAVATRLSLQRE
                     AEALVLEAPFTSVVDVGKLLWPLLPLELIMTDQYRTIDRIDQVDIPLFIVHGGRDSVI
                     PLYQARRVYHAASEPKTLQVLPRAGHNDLFEMGAWGQVRAFLAGLRPEPVVAEPKVRA
                     GHPIGLEAAADASR"
     gene            complement(2661..2894)
                     /gene="rpsU"
                     /locus_tag="AC16Y7_05810"
     CDS             complement(2661..2894)
                     /gene="rpsU"
                     /locus_tag="AC16Y7_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR5708930.1"
                     /translation="MQVVVRDNNVDQALRALKKKMQREGIFREMKLRNYYEKPSEKRA
                     REKAEAIRRARKLARKKAQREAGTSSGRSRGRR"
     gene            complement(3075..>3572)
                     /locus_tag="AC16Y7_05815"
     CDS             complement(3075..>3572)
                     /locus_tag="AC16Y7_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR5708931.1"
                     /translation="GQDGETRFYDVVENVHQGGILATSRVPASISDSVVFEARKIAGT
                     IAEALNYVGVLCVEMFQREGEEPGLIVNEFAPRVHNSGHWTIDACRVSQFENHVRAIC
                     GWPLGDVSRHSNAVMTNLIGDDVEAWPALAAEPGLGLHLYGKAEARPGRKMGHVTRLT
                     PKTSD"
BASE COUNT          606 a         1122 c         1135 g          709 t
ORIGIN      
        1 catcctggcg ctgcgcgggc tgtcttcgcc cgagacctcg cgccagggca actacatggg
       61 catggccggt atggccatcg ccgttgccac cacgctcgcc gtggcgcagc ccgagagtgc
      121 gctctcctgg gcgatgatcg tcggcggtat tgccgtcggt ggtgccatcg gtgcggtgat
      181 cgcacggcgc attgccatga cggcgatgcc cgaactggtc gccgcgttcc actcgctggt
      241 cggtatggcc gcggtgcttg tcgcggcagc cgcgtactac gcgcctgagg ccttcggcat
      301 cggcgcgacc ggcgctatcc gtgtggcgag cctgatcgaa atgtcgctcg gcgcggccat
      361 cggcgccatc accttcactg gttcggtcat tgccttcgcc aagctgtcgg gccgcatgtc
      421 cggcgcgccc atcctgctgc cggcgcggca tctcatcaac atcacgttgg tcatcgggct
      481 ggtggcctgc atcgtcctgt tctgtcagga gcaaacgccg gcctatttct gggcgatcgt
      541 agggctgtcg ctgctcctcg gcgtgctcat catcatcccg atcggcggcg cggacatgcc
      601 ggttgtcatc tcgatgctca actcatactc gggctgggcg gccgccggca ttggcttcac
      661 gctaggcaac acggcgctga tcgtcactgg cgcgctggtc ggctcgtcgg gcgcgatcct
      721 atcctacatc atgtgcaaag gcatgaaccg ctcgttcatc agcgtcatcc tcggtggctt
      781 cggcggcgag atggcggggc ctgctggtga cggcgagcag aagccggtga agctcggttc
      841 ggccgaggac gcagcttacc tcatgaagaa tgcgagccgc gtgatcatcg tacctggcta
      901 cggcatggcg gtcgcgcaag cgcagcatgc tttacgcgag atgggcgatg gtctgaagga
      961 ggcaggtgtg gaggtgaagt acgccattca ccccgtggcc ggccgcatgc caggccacat
     1021 gaatgtactc ctcgccgaag cgaacgtgcc ttacgacgag gtgttcgagc tggaagacat
     1081 caatagtgaa tttccgcaaa ccgacgtggt gtacgtgatc ggcgccaacg acgtggtgaa
     1141 cccggcggcg gaggacgacc cgtcctcgcc gatctacggc atgccggtgc tacaggtgtg
     1201 gaagtccggc accgtactgg tcaacaagcg gtcgctggcc tccggctatg cgggcatcga
     1261 caatcccgtc ttctacgggg acaacacgat gatggtgctc ggcgacgcca agaagatgac
     1321 ggaagacatc gccaaggcgc tgtaggttgc cggcaaccat gcggcgtagt taacggtcca
     1381 aaagataacg aagaatcgtc aaagtcgagc ctagattcgc ccaactccgg ctcaaatcca
     1441 cccttaaacg gctgactgca cgacccgtcg gtcccggcat ggctttggct tgcccgcgtc
     1501 gacgccgccg agattggcgc cgcttagctc gcagttaagc ttcgtggggt tgggtccacc
     1561 cgaaattcct ttgattggcg cagcaattgc tacagcgcat atgcgccgtt gaagatctct
     1621 ttggctgagc ggggtgttca agcaccgttc cgtccggcca gcctagcgtt ggccaagaac
     1681 aagaaaatgc agcgaggccg tgactaactt atgttgaacc tgttcctctc atttgccggc
     1741 atatttgcgc tcatctgcct cggcgggcgg ttgctccatc gctacttcgt gtacatcccc
     1801 gatccccggc gcatcgctcc gaaggctgca gggctcgtct ccgtcgacga gatcgtcttc
     1861 aaggctgccg atgggacgcg cctgatcgcg tggtatcgcg aagcgcagcc cggcaagccg
     1921 acgcttctgt atttcaccgg caatagcggc aacaccgccg atcgcgccag caagatcaag
     1981 gcgatcggcg ccgacggcta tggcgtgttc atgctgaact accgccgttg tggtggctcg
     2041 ggcggaaacc cgaccgaaaa gcgcatcacc gcggatgcgg ttagcgccta tgactgcttg
     2101 cgtgggttgg gcgtcgcgcc gcgcgatatt gtcgtctatg gcgaatcatt gggtacggcc
     2161 gtggccacgc ggctcagcct tcagcgcgag gccgaggcgc tggtgctcga ggcgccgttc
     2221 acctcggtgg tggatgtcgg caagctgctt tggccgctcc tgccgcttga actcatcatg
     2281 accgatcaat accgcaccat cgatcggatc gatcaggtcg atatcccgct cttcatcgtt
     2341 catggcggcc gggacagcgt gatcccgctc tatcaggccc ggcgtgtcta tcacgcggcc
     2401 agtgagccga agacgcttca ggttctgccg cgggcgggtc acaacgatct gttcgagatg
     2461 ggcgcttggg gccaggtgcg tgccttcctc gcaggcctcc ggccggagcc tgtggtggcg
     2521 gagcccaaag tgcgggccgg ccacccgatc gggctcgagg cggctgcgga cgcgagccgc
     2581 tagagcggcc tgccaaacgc aacaaagccg ggaaggaact tcccggcttc accagcttcg
     2641 atcttagcga agaaaagcgc ctagcggcgg ccgcggctcc gaccggaaga ggttccggct
     2701 tcgcgttgcg ccttcttgcg tgcgagcttg cgggcgcggc ggatcgcctc ggccttctcg
     2761 cgggcacgct tctcggaggg cttctcgtaa taattacgga gcttcatctc gcggaagatg
     2821 ccttcacgct gcattttctt cttcagggcc cggagggcct gatcgacatt gttatcgcgg
     2881 acgacgactt gcacgcgtct ataatctccg aattagcgtt cattttggga ttgaagcgaa
     2941 cccaaatcga cccgagcaga gccgatctca gtcccgcatc aggggctgat ctagcaaaag
     3001 agcccttcgt tgtccaggct ggtgccaggg tcttccggcc cattctctag accgaggtct
     3061 ctagtgcgag gcctctaatc tgaggttttg ggcgtgaggc gcgtgacatg gcccattttg
     3121 cgccccgggc gggcttctgc cttgccgtag aggtgcagac ccaagccggg ctcggcggcg
     3181 agcgcgggcc aggcctccac gtcgtcgccg atcagatttg tcatcaccgc attgctgtgg
     3241 cggctcacat cgcccagcgg ccagccgcaa atggcccgca catggttctc gaactggctg
     3301 acgcggcagg catcgatggt ccagtgcccc gaattgtgca cgcgcggggc gaactcattg
     3361 acgataagtc cgggctcttc gccctcgcgc tggaacatct ccacgcacag cacgcccaca
     3421 tagttgagag cctcggcgat ggtgccggcg atcttccgcg cctcgaacac gaccgaatcg
     3481 gaaatgctgg cggggacccg gctcgtggcg agtatgccgc cctggtgcac gttctcgacc
     3541 acgtcgtaga agcgcgtctc gccgtcttgc cc
//
LOCUS       DBNHVU010000144         4683 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1985212, whole genome shotgun sequence.
ACCESSION   DBNHVU010000144 DBNHVU010000000
VERSION     DBNHVU010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1985212"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="AC16Y7_05820"
     CDS             <1..618
                     /locus_tag="AC16Y7_05820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018327059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5708932.1"
                     /translation="LNLIVSGLALHRVNDLPGSLIQIRRALAPDGLFMAAALGARALI
                     ELREVLIEAEGEITGGASPRVSPFGDVSAYGALLQRAGFALPVADSEMLTVTYPSPRA
                     LMREVRALGGGNVLTARSKAPLSRRTLGRAEELYRARYGTPDGKVTATFEFVFMSGWA
                     PHESQQKPLKPGSAQARLADALHTEERPAGDKASFGTSADAKGKG"
     gene            complement(615..824)
                     /locus_tag="AC16Y7_05825"
     CDS             complement(615..824)
                     /locus_tag="AC16Y7_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006472818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HIR5708933.1"
                     /translation="MTAETTKVADFKTRFVAEARRFASEPSGATAIEYAIIAAMLSIV
                     IIGAVAALGEGVTGLFQRVANAFSS"
     gene            complement(821..1258)
                     /locus_tag="AC16Y7_05830"
     CDS             complement(821..1258)
                     /locus_tag="AC16Y7_05830"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007632760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HIR5708934.1"
                     /translation="MADGTTKPVVLVVACALIDVEGRLLLTKRPEGKALTGLWEFPGG
                     KVEPGETPEHAIIRELEEELGITMSVAGLTPLTFASYPYTEFHLLMPVFGCRRWQGTV
                     VPREGQAVAWVGPNALSGYAMPPADEPLKETLPRLLKLWREER"
     gene            complement(1265..2503)
                     /gene="argJ"
                     /locus_tag="AC16Y7_05835"
     CDS             complement(1265..2503)
                     /gene="argJ"
                     /locus_tag="AC16Y7_05835"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015917285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HIR5708935.1"
                     /translation="MAKISPFAPERLAELPPIDGVRMASCAAGIRYAGRTDLLLALFE
                     PATTVAGVLTQSKTASAAVEWCRQHLRHGMARALVVNSGNANAFTGMRGSVAVKETAH
                     AAADVADCLDADVYIASTGVIGEPLEPSKFVARLAELARSATPDGYEAAAQAIMTTDT
                     FPKLATRTCKIDGVDITLNGIAKGAGMIAPDMATMLSFIFTDAAIEPGALHAILAPLV
                     EDSFNAITIDSDTSTSDTVLLFATGTATKRGCPRIANGDDPRLDDFRAALAALMRDLS
                     HQIVKDGEGLTKFVKVTVTGAETKAAARVIAKAIANSPLVKTAIAGEDPNWGRIVMAV
                     GKAGEAADRDKLAIRFGDIEVAKDGEAAPDYVESDAAAYMRGPEIEITVDVGVGAAQD
                     TMWTCDLTKEYIAINADYRS"
     gene            complement(2521..3396)
                     /locus_tag="AC16Y7_05840"
     CDS             complement(2521..3396)
                     /locus_tag="AC16Y7_05840"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR5708936.1"
                     /translation="MKIWSAMLLLAASVSLVPFAAAAEDAVIARVNGVEIKQSDLDFA
                     ASEVGPRLGSFRPDDRQRVLLQFVIENELMAAAGEKDKLNKADNFDARVKYHTRRALR
                     DAYFDSSITGAVKDADAKKIYDEKLAGMKPAQEVHARHVLVATKEEAEAVKKRLEKGD
                     DFAKVAEELSKDTNAKGGDLGYFSRGQMLKPFEEAAFSLDVGKISDPVETQFGWHVIK
                     VEDKRDQKPPNFDDVKQAILTQLVTQKAQAVVSGLRNSAKIEIVDPKIKRSMDDAALR
                     GEAPPLPDEEFKEDH"
     gene            3637..3900
                     /locus_tag="AC16Y7_05845"
     CDS             3637..3900
                     /locus_tag="AC16Y7_05845"
                     /inference="COORDINATES: protein motif:HMM:NF024248.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5708937.1"
                     /translation="MAAGDIARTLQVPHNTLSSQLAILSRAQLIQFRKDGRSMIYTVD
                     LQGTRALLCFLVEDCCQGEPSVCSPLIESALAGCCASQGGNAS"
     gene            3897..4427
                     /locus_tag="AC16Y7_05850"
     CDS             3897..4427
                     /locus_tag="AC16Y7_05850"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HIR5708938.1"
                     /translation="MTAPNKNEPLNVLFLCTGNSARSILAECILNREGRGKFRAYSAG
                     SMPTGEVNPIALGLLRKLNYEVSGLRSKSWDDFAGPDAPEFDFVFTVCDDAANEACPV
                     WPGQPMTAHWGLPDPAKAKGTDAERGLAFDDVYRMLHQRISILVSLPLDSLSKLALQE
                     HLDQIGKTGQSAKEPA"
BASE COUNT          838 a         1537 c         1408 g          900 t
ORIGIN      
        1 ctcaacctga tcgtttcggg gctggccctt caccgcgtca acgatcttcc cggcagtctg
       61 atccagatcc gacgcgcgct ggcgccagac ggtctgttca tggctgcggc cttaggcgcg
      121 cgcgcgctga tcgaactacg cgaagtgctg atcgaagcgg aaggcgagat aacgggcggc
      181 gcctccccgc gcgtctcgcc ctttggcgac gtgagcgcct atggcgcgct tctgcaacgc
      241 gcaggctttg ccctgccggt ggcggattca gaaatgctga ccgtcaccta tccttcgccg
      301 cgtgcgttga tgcgcgaggt gcgtgcgctc ggcggcggca atgtgctgac ggcgcgcagt
      361 aaggcacccc tgtcgcgccg cacgttgggg cgcgcggagg agctctatcg cgcgcgatac
      421 ggaacgcccg acggaaaggt caccgcgacc ttcgagttcg tgttcatgag tggctgggcg
      481 ccgcatgaaa gccagcagaa accgctgaag cccggcagcg cacaagcacg gctcgcggat
      541 gcgctccaca ccgaggaacg cccggccggc gacaaggcgt cgttcggcac atcggccgat
      601 gcgaaaggga agggctagct ggaaaaggcg ttggcgacgc gctggaacag gcccgtgaca
      661 ccctcgccca gagcagcgac agctccgatg atcacgatac tgagcatggc tgcaatgatg
      721 gcatactcga tggccgttgc gcccgacggc tcagacgcaa agcggcgagc ctcggcgaca
      781 aaccgtgtct tgaagtctgc gacctttgtc gtctctgccg tcatcgctct tctcgccaca
      841 gtttcaaaag acgcggcaac gtctctttca gcggttcgtc cgccgggggc atggcatatc
      901 cggacaacgc attcggccct acccaggcaa ctgcttgccc ctcacgcggg accacggttc
      961 cctgccagcg ccggcagcca aataccggca taagcagatg aaactccgta tagggataac
     1021 tcgcaaaagt taaaggagtg agtcctgcaa ccgacatcgt gatgccgagt tcttcttcca
     1081 gctcgcggat gattgcatgt tccggcgttt caccgggctc gaccttcccg cctgggaact
     1141 cccagagacc cgtgagagcc ttgccttccg gacgtttcgt gagaaggaga cgcccctcca
     1201 cgtcaatcag cgcgcaggcc acgacaagca cgacaggctt tgtcgtccca tctgccatct
     1261 tggtttacga ccggtaatcg gcgttgatgg cgatgtactc cttggtgaga tcgcaggtcc
     1321 acatcgtatc ctgcgcggcg ccgacaccga catcgacggt gatctcgatc tccggccccc
     1381 gcatataggc ggcagcgtcc gattccacat agtccggtgc ggcttcgcca tccttcgcga
     1441 cctcgatgtc gccgaagcga atcgcaagct tgtcgcggtc cgccgcctcg ccagctttgc
     1501 caacggccat gacgatgcgt ccccaattgg ggtcctcgcc ggcgatcgcc gtcttgacca
     1561 aaggcgagtt cgcgatcgcc ttcgcgatga cgcgcgcggc cgccttggtt tccgcacccg
     1621 tgacagtcac cttgacgaac ttggttagcc cctcgccgtc cttgacgatc tggtgcgaaa
     1681 gatcgcgcat gagcgcggcc aaggccgcgc ggaaatcgtc gaggcgcgga tcatcgccgt
     1741 tcgcaatacg cgggcagcca cgcttcgtag cagtgcccgt cgcgaacagg agcaccgtgt
     1801 cgctggtcga agtgtcgctg tcgatggtga tggcgttgaa actgtcctcg acgagcggcg
     1861 ctagaatcgc gtgcagcgcg ccgggctcaa ttgcggcatc agtgaatata aaggacagca
     1921 tggtcgccat gtcgggcgca atcatgcctg cgcctttggc gatgccgttc agcgtgatgt
     1981 ctacgccatc gatcttgcat gtgcgggtcg caagtttggg gaaggtgtcc gtggtcatga
     2041 tcgcctgcgc cgccgcctca tagccatccg gcgtcgcgct ccgcgcaagc tcggcgagac
     2101 gggccacgaa cttggacggc tccaacggct cgccgatcac ccccgtcgag gcgatgtaga
     2161 cgtcggcgtc caggcagtcg gcgacgtctg cggcggcgtg cgccgtctcc ttcaccgcca
     2221 cggacccacg catgccggtg aacgcattgg cattgccgga attgacgacg agcgcgcgcg
     2281 ccatgccatg gcgcaaatgc tggcggcacc attcaacggc ggcagaggcg gtcttcgatt
     2341 gcgtaagaac gcccgccacc gtggtcgccg gctcgaacag cgccaacaac aagtcggtgc
     2401 gtcccgcata gcggattccg gcggcgcacg aagccatgcg cacaccgtca atcggcggca
     2461 attccgctag gcgttctggc gcaaaaggcg agatcttggc caaggctcac cgcttggagc
     2521 ttagtggtct tctttgaact cttcgtcagg cagcggaggc gcctcgccgc gcaaggcggc
     2581 atcgtccatg gaccgcttga tcttaggatc gacaatctcg atcttggccg aattgcgcaa
     2641 gccggagacg accgcctgcg ccttctgcgt cacgagctgg gtcaggatgg cctgcttgac
     2701 gtcatcgaag ttcggcggct tctggtcgcg cttgtcctcg accttgatga cgtgccagcc
     2761 gaactgggtc tctacgggat cggaaatctt gcccacgtcg agcgaaaagg ccgcttcctc
     2821 gaacggcttc agcatctgac cgcggctgaa atacccgaga tcgccaccct tggcgttcgt
     2881 gtccttggat agctcctcgg ccaccttcgc gaagtcgtcg cctttctcca ggcgcttctt
     2941 gacggcctcc gcttcttcct tggtggcaac cagaacatgc cgggcatgca cttcctgcgc
     3001 cggtttcatg ccagcgagct tctcgtcgta gatcttcttg gcgtcggcgt ccttcaccgc
     3061 gcccgtgatg ctcgagtcga aatacgcatc gcgcagcgcg cgccgcgtgt gatatttgac
     3121 ccgcgcatcg aaattgtcgg ccttgttgag cttgtccttc tcgccggcgg cggccatcag
     3181 ctcgttctcg atgacaaatt gcagcagcac gcgctggcga tcgtccgggc ggaaactgcc
     3241 gagccgcggt cccacctccg aagcggcgaa gtcgagatcc gattgcttga tttcgacacc
     3301 gttcacccgg gcaatgaccg cgtcttcggc agcggcggcg aacggaacga gagagacgga
     3361 cgctgccaga agcagcatgg ctgaccaaat tttcacagga tgaattcctc gtattctgcc
     3421 cgtccgtgtc gcgccggcgg gccggttggg tgctttcccc gcgccatgtt tagccttggg
     3481 cccgcccatt gccaagcggg ggaattgacc gcaccccatt tattctatat ttctggacat
     3541 atggacacac caatcgctct aactgctctc ggcgccctgg ctcaggaggg caggctgaag
     3601 gtcttccgcc ttctcgtccg cgccggaacc gagggaatgg cggccggcga catcgcccgc
     3661 acgcttcagg tcccccacaa cacactgtca tcccagctcg ctatcctgtc gcgcgctcag
     3721 ctgatccaat tccgaaaaga tggccgctcg atgatttaca cggtcgacct gcaaggcacg
     3781 cgagccctgc tgtgtttctt ggtggaggat tgttgtcagg gagagccatc cgtttgctcg
     3841 ccgctgattg agtccgccct ggctggttgc tgtgcaagcc aaggaggaaa tgcctcatga
     3901 ccgcccccaa caaaaatgag ccgctcaacg tgctcttcct atgcaccggc aactcggccc
     3961 gctcgatcct ggcggagtgc atcctgaacc gcgagggccg aggcaaattt cgcgcctaca
     4021 gcgcgggcag catgccgacc ggcgaggtga accccatcgc actcgggctc ttgcgcaagt
     4081 tgaattatga ggtttcgggc ctgcgctcga agtcctggga cgacttcgcc ggacccgacg
     4141 cgcccgagtt cgacttcgta ttcacggtct gcgacgacgc cgccaatgag gcctgcccgg
     4201 tctggccggg gcagcccatg acggcgcatt ggggcctgcc ggatccagcc aaggccaagg
     4261 gcaccgacgc cgagcgcggc ctggccttcg acgacgtcta ccgcatgctc caccagcgca
     4321 tctcgatcct ggtgagcctg ccgctcgata gcctcagcaa gctcgcgctc caggagcatc
     4381 tcgaccagat cggcaagacg gggcaatccg ccaaggagcc cgcctagaga cccgccttgc
     4441 cctccgcgtc gcgttgacaa gggtaagggc gcctcttatg tctcaaacgc gttgatcccc
     4501 ggcatttttc gtcaatttcc caagaggttt ggcgagcatc gaccggggat tgccgcactc
     4561 aagcagccag ggaccttgaa gggctttacc taagccccac ttggcctggc tcaccccata
     4621 cgaggcttaa agatcgcgat gctttccttc ggcactctcg ccaccaaagt cttcggctcc
     4681 tcc
//
LOCUS       DBNHVU010000145         6187 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1538617, whole genome shotgun sequence.
ACCESSION   DBNHVU010000145 DBNHVU010000000
VERSION     DBNHVU010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1538617"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="AC16Y7_05855"
     CDS             <1..857
                     /locus_tag="AC16Y7_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR5708939.1"
                     /translation="GSVFKLFTVAGVLDLGLANLNTTYDATSPIKFGRFSISDFHGKK
                     RPLSVTESFIYSSNIATAKMALHMGVNRHKAFLKKLGLLNSVTTELGPSAAPIVPKHW
                     KKLNTMTIAFGHGLSVTPLQLATATVPLVNGGLSVPPTFLPRTREEAMQVATRVLKPG
                     TSEAINKLMRMNVVKGTGKRADAEGYRVGGKTGTAEKVVNGRYARSALLNSFLATFPT
                     DDPQYVVLVTLDEPQRVAATNNAYTAGVNAAPTVGKVVARIAPILGVPPKLEAGQDRF
                     DAKVSATY"
     gene            935..2392
                     /locus_tag="AC16Y7_05860"
     CDS             935..2392
                     /locus_tag="AC16Y7_05860"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR5708940.1"
                     /translation="MTLGELIGPEAKLPASLAATPIKGLAADSRQVAPGYLFAALPGV
                     NADGTRFVADAVSRGAAAILVPEGVHPETSAPVIADADPRRRLALIAARFFGAQPETA
                     VAVTGTNGKTSVASFVRQLWAARGFEAASLGTIGVVTPRGTKVLRHTTPEPIELQGLL
                     AELAKGGVTHLALEASSHGLQQRRIDGVTLAAGAFTNISRDHLDYHATFEDYFTQKLR
                     LFTTLLAPGAAAVIDMDGDGSVRVADVANAHGLDVMTVGRAGETLRLVSDDIDGFAQV
                     LGIEHGGKRHDVRLPLVGGFQAANALVAAGLVMATGANAADVLPHLENLQGARGRLDL
                     AGTAPAGAPIFVDYAHTPDALAKALDALRPYVRNRLLVVFGCGGDRDKGKRPEMGAAS
                     VAKANIAIVTDDNPRNEDPGDIRREILAAAPGAIEIGDRAEAIVEAVAMLEKGDVLLV
                     AGKGHETGQTVGTTVIPFSDHEAVSAALNDEAKLG"
     gene            2385..3803
                     /locus_tag="AC16Y7_05865"
     CDS             2385..3803
                     /locus_tag="AC16Y7_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamyl-2,
                     6-diaminopimelate--D-alanyl-D-alanine ligase"
                     /protein_id="HIR5708941.1"
                     /translation="MADPLWTLGEILVATGGRSEGNPASRVSGFSIDSRSLKSGEGFI
                     AIRGLNRDGHEFIPAALDAGAACAVVEETYHPSDEQRLVRVTDTFDALNDLGRAGRDR
                     AEAAVVIAVTGSVGKTGTKEALRRALEPSGTVHASSKSYNNHWGVPLTLANMRRDIAY
                     GVFEVGMNHAGEIDALTKLVRPHIAIVTTVAPVHLGFFDSVEEIADAKAEIFHGLEPG
                     GAAIINRDNSHYERLAARARKNGAEIVSFGEDEGANARLISADLTPESSQVRADIMGE
                     TVDYRLGAPGRHLVQNSLAVLAAVKLAGADLAKAAEAFGVMQPQAGRGERRVIQTKSG
                     PVAIVDESYNANPASMRAALATLGLAPRDAYKRRVAVLGDMLELGAEGPKLHAGLAEA
                     VDEAGVDVVFACGELMDSLFQALPAGRQGGYAKSSEELSPKLLEAVGPGDVIMVKGSL
                     GSRMAPLVEALTRHFEAESVSA"
     gene            3828..4916
                     /gene="mraY"
                     /locus_tag="AC16Y7_05870"
     CDS             3828..4916
                     /gene="mraY"
                     /locus_tag="AC16Y7_05870"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR5708942.1"
                     /translation="MLYWLAELAPDSSVFNVFRYITFRTGGAVLTALLFVFLFGPRVI
                     RSLRVRQGKGQPIRKEGPERHILQKQGTPTMGGLMILSGVAASVLLWGNLGNPYVWVV
                     LLLTLVYGAVGLFDDFLKVTRQSADGFRGRIKIVVETAAALVATYVIASFGEEPFSTS
                     IAIPFFKDFMIPLGKWLFIIFGTFIIVGAGNAVNLTDGLDGLAIVPVMIAAATFGFIA
                     YVVGNALFADYLQIHFVAGTGELAIVCGALVGAGLGFLWFNAPPALIFMGDTGSLALG
                     GALGTIAVATKHELVLAIVGGLFVLEAASVIVQVFSFKMFGKRVFRMAPLHHHFEQKG
                     WPESTVVIRFWIISVVLALAGLATLKLR"
     gene            4929..>6187
                     /gene="murD"
                     /locus_tag="AC16Y7_05875"
     CDS             4929..>6187
                     /gene="murD"
                     /locus_tag="AC16Y7_05875"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR5708943.1"
                     /translation="MIPVTIFAGSEVAVVGAGLSGLATARALDVGGANVVVWDDKLKA
                     RDEAEAAGFAVRDLPAADFSKFDALVLAPGIPLTHPEPHWSVRKAKGAGIEVIGDVEL
                     FLRERAKSGAGSKVIAITGTNGKSTTTALAAHLLDTAGKQVALGGNIGKAVLDLQSFT
                     PDMTYVLELSSYQIDLTPSLAPDAAALLNVTPDHLDRHGTLQNYAAVKARIFAQVPPD
                     GTAVIGVDDESCRAIADDLRGSFAVKRIAVGHAVEDGVWAKDGVLLEMEGGQEVGRAD
                     LAGIGSLRGAHNWQNAAAAYALARAMGLDGAAIQQGLKSFVGLAHRMEQVARVGNVLF
                     VNDSKATNADAAGKALGSFDDIYWIVGGRAKDGGLAGLEAFYPKITRAYLIGEAAEDF
                     AHQLGDAVPHVVCGTLDRAVTDAARDAG"
BASE COUNT         1061 a         1927 c         2024 g         1175 t
ORIGIN      
        1 tgggctccgt gttcaagttg ttcaccgtgg cgggcgtgct cgatctgggg ctcgccaatc
       61 tgaacacgac atatgatgcc accagtccca tcaagttcgg ccgcttctcg atcagcgact
      121 ttcacggcaa gaagcgcccg ctcagcgtca cggaatcgtt tatctactcg tccaacatcg
      181 ccacggcgaa gatggcgctg catatgggcg tgaaccgtca caaggcgttt ctgaagaagc
      241 tcggtctcct gaactccgtg accacggaac ttgggccgag cgcggcgccg attgttccga
      301 agcattggaa gaagctgaac acgatgacca tcgcgttcgg tcatgggctg tcggtgacgc
      361 cgcttcaact ggccacggca acagtgccgc tcgtcaacgg cggtctttcc gtaccgccga
      421 catttctgcc gcgcacgcgc gaggaggcga tgcaggtcgc aacgcgcgtg ctgaagcccg
      481 gcaccagtga agcaatcaac aagctgatgc gcatgaacgt ggtcaagggc acaggcaagc
      541 gcgccgacgc ggaaggctat cgtgtcggcg gcaagacggg tacggcggaa aaagtcgtca
      601 atggccgcta tgcacggtcg gcgttgctca acagtttcct ggcgaccttt ccgaccgacg
      661 atccgcaata tgttgtcctc gtcacgctgg acgaaccgca gcgcgtcgcc gcgacgaaca
      721 atgcctatac ggccggcgtg aatgcggccc cgaccgtcgg taaggtcgtg gcccgcatcg
      781 cgcccatcct cggggtgccg cctaagctcg aagccggtca agaccggttt gacgccaagg
      841 tttccgccac ctattagaag actccccctt gtcctgaggc gtgcgcgcct ttggaattat
      901 tcttagataa cagccggttg ccggcaatcg aaccatgacc ttgggtgagt tgatcggacc
      961 ggaggcgaag ctgccggcat cgctggctgc aacgccgatc aaggggcttg ccgcggacag
     1021 ccggcaggtc gcgcccggct atctcttcgc ggcgcttccc ggtgtgaatg cggatgggac
     1081 acggtttgtc gccgacgccg tgtcgcgcgg cgcggccgct attctcgtgc ccgaaggtgt
     1141 gcatccggag acgagcgcgc cggtgattgc ggacgccgat ccgcgccggc ggctcgcgct
     1201 gatcgccgcg cgttttttcg gagcgcagcc cgagaccgcc gtggcggtca ctggcaccaa
     1261 cggcaagaca tccgttgcgt cgttcgtgcg tcagctctgg gcggcgcgcg gtttcgaagc
     1321 ggcaagcctt ggcacgatcg gtgtcgtcac accgcgcggc acgaaagtgt tgcgccacac
     1381 gacgcccgaa cccatcgagc ttcaggggct tcttgcggag ctcgctaaag gcggggtcac
     1441 acatctcgct ctcgaagcgt caagccatgg tctgcagcaa cggcgcatcg atggcgtgac
     1501 gttagcggcg ggcgccttca ccaacatttc gcgcgaccat ctagattatc acgcgacgtt
     1561 cgaagactac ttcacgcaga agctccgcct gttcacgacg ctgctcgcgc cgggcgccgc
     1621 cgctgtcatc gacatggatg gcgatggaag tgtgcgtgtg gccgatgtcg cgaacgcgca
     1681 cgggctcgat gtcatgactg tcgggcgtgc cggggagacc ttgcgtctcg tctccgacga
     1741 cattgacggc ttcgctcaag tgctcggcat cgaacacggc ggcaagcgtc atgacgtgcg
     1801 tctgccattg gtgggcggct tccaagcggc caacgcgctt gtcgcggcgg gtctcgtcat
     1861 ggccacgggc gcgaacgccg ccgacgtgct gccgcatctt gagaacctac aaggcgcgcg
     1921 cggccggctc gatctggccg gcaccgcgcc ggccggtgcg cccattttcg tagattacgc
     1981 tcacacgccc gatgcactgg ccaaggcgct cgatgcgttg cggccctatg tcaggaacag
     2041 gctccttgtt gtgttcggtt gcggcggcga ccgcgacaaa ggcaagcgtc ccgagatggg
     2101 tgccgcctca gtcgccaagg ccaacattgc catcgtcacc gacgacaatc cgcgcaacga
     2161 ggacccaggc gatatccgcc gcgagattct tgccgcggcc cccggggcca tcgagatcgg
     2221 cgaccgcgct gaggccattg ttgaagccgt tgccatgcta gagaagggcg acgtgctgct
     2281 cgtggccggc aagggacatg agaccggtca aactgtcggc acgacggtca ttccgttctc
     2341 cgaccacgag gcggtgtccg ccgcgctaaa cgatgaggcg aaacttggct gatccgctct
     2401 ggaccttagg agagatcttg gttgccaccg gcggtcgcag cgaaggcaat cccgccagca
     2461 gggtttccgg tttctccatc gacagccgca gcctgaagtc gggtgagggg ttcatcgcca
     2521 ttcgcggtct caatcgggat ggtcatgagt tcataccggc cgcgctcgat gcaggcgctg
     2581 cctgcgccgt ggtggaagag acctatcacc cgagcgatga acaacgcctg gtgcgggtga
     2641 ctgacacgtt cgacgcgctc aacgatctcg gccgcgcggg ccgagatcgg gcagaagccg
     2701 ccgtcgtgat tgccgtgacc ggcagtgtcg gcaagacggg caccaaggag gctttgcgcc
     2761 gcgcgcttga gcccagcggc acggtgcatg cctcctccaa gtcctacaac aatcactggg
     2821 gcgtgccgct cacgctcgcc aacatgcgaa gagacattgc ttatggcgtg ttcgaggtcg
     2881 gtatgaacca cgccggcgag attgacgcgc tgacgaagct ggtccggccg cacatcgcca
     2941 tcgtcaccac cgtggcgccg gtgcatttgg gcttcttcga ctccgtggaa gagattgccg
     3001 atgccaaggc cgagatcttt cacgggctcg agccgggcgg cgcggcgatc atcaatcgtg
     3061 acaattcgca ttatgagcgg ctggcggccc gtgcgcgcaa gaacggggct gagattgttt
     3121 cgttcggcga ggacgaaggc gcaaacgcaa ggttgatctc ggcggatctc acccccgaaa
     3181 gctcgcaagt gcgcgccgat atcatgggcg agacggtgga ctaccgtctc ggcgcgccag
     3241 gacgccatct cgtccagaac tcgcttgccg tgctcgcggc ggtaaagctg gcgggtgccg
     3301 accttgcaaa ggcggccgag gcgtttggcg tcatgcagcc ccaagccggc cggggggagc
     3361 gccgcgtcat ccagacgaaa tcggggccgg tggccatcgt cgatgagtcc tataatgcca
     3421 acccggcctc catgcgggcg gccctggcga cgctcggact tgcgccgcgc gatgcctata
     3481 agaggcgggt cgcggttctg ggggatatgc tggagctggg cgcggaggga ccaaagctgc
     3541 atgccgggct cgcggaagcc gttgacgagg cgggggtaga tgttgtcttt gcctgcggcg
     3601 agcttatgga ttcactgttc caggcgctcc ccgcggggcg ccagggcggc tatgctaagt
     3661 cctcggagga gctttctccg aaattgctcg aagcggtggg gccaggggac gtcatcatgg
     3721 tcaaggggtc tttaggcagc cgcatggccc cgctcgtcga ggcgttgacg cgccatttcg
     3781 aggcggaaag cgtttcggcg taaggcgccg aagaaggggg aaaggcgatg ctctattggt
     3841 tggccgagct ggcgccggac tcctctgtct ttaatgtctt ccgctacata accttccgga
     3901 cgggaggcgc ggtcctcacc gcgcttctgt tcgtcttctt gtttgggccg cgggtaatcc
     3961 ggtccttgcg tgtccgtcag ggcaaaggcc agccgatccg caaagaagga cccgagcggc
     4021 acatcctgca gaagcaggga acgccaacca tgggcgggtt gatgatcttg tccggcgtcg
     4081 cggcctcggt gctgctatgg ggcaatctgg gcaaccctta tgtgtgggtc gtgctgctgc
     4141 taacgcttgt ctacggcgcc gtcggcctct tcgatgattt cctgaaagtc accaggcagt
     4201 cggcggacgg cttccgcgga cggatcaaga tcgtcgtcga aaccgccgcc gcgctcgtcg
     4261 ccacctatgt cattgcgtct tttggcgagg aaccgttttc cacttcgatc gccattccgt
     4321 tcttcaagga cttcatgatc ccgctgggca aatggctgtt cattatcttc gggacgttca
     4381 tcatcgtcgg cgcgggcaac gcagtgaacc tgaccgatgg tctcgacggg ctcgcgatcg
     4441 tgccggtgat gatcgccgca gcgacgttcg gcttcattgc ctatgtagtc ggcaacgcgc
     4501 tcttcgccga ctaccttcag atccacttcg tggccgggac gggtgagctt gccattgtct
     4561 gcggggcgct cgtcggcgca ggactcggat tcttatggtt caacgcgcca cctgcgctga
     4621 tcttcatggg cgataccggc tcgctggcgc tcggcggggc gctcggcacc atcgccgtcg
     4681 ccaccaagca tgagctggtg ctggccattg tcggcggtct gttcgttcta gaggccgcct
     4741 cggtgatcgt gcaggtcttc tccttcaaga tgttcggcaa acgcgtgttc cgcatggcgc
     4801 cgctgcacca ccatttcgaa cagaagggat ggcccgaatc cacggtcgtt atccgcttct
     4861 ggattatctc tgtggtgctg gctctggccg gtctcgcgac gctgaaattg cgctaagttc
     4921 aggccatcat gattcccgta acgatcttcg ccggcagcga ggttgcggtc gttggagcag
     4981 ggctaagcgg ccttgcgacc gcccgcgcgc tggacgtcgg cggcgccaac gtcgtggtct
     5041 gggacgacaa gctgaaggcg cgcgatgagg cggaagccgc aggtttcgcc gtgcgcgacc
     5101 tgccggccgc cgacttttcg aagttcgacg ccctcgtcct tgcgccgggc atccctctga
     5161 cccatcccga gccgcattgg agcgtgagga aggcaaaggg cgccgggatc gaagtcatcg
     5221 gcgatgtgga gctgttcctc cgcgagcgcg ccaaatccgg cgccggcagc aaagtcatcg
     5281 ccatcaccgg caccaacggc aaatccacca ccacggcgct tgccgcgcat ctgttggata
     5341 ccgccggcaa gcaggtggcc cttggcggta atatcggcaa ggcggtgctg gaccttcagt
     5401 ccttcacccc cgacatgacc tatgtcttgg agctgtcctc gtaccagatc gacctgacgc
     5461 cctcgcttgc gcccgacgcc gcggcgcttc tcaacgtcac accggatcat ctcgaccggc
     5521 atggcacgct tcagaactac gccgccgtca aggcgcgcat ctttgcgcag gttccgccgg
     5581 acggaaccgc cgttatcggc gtcgacgacg agtcctgccg ggctatcgcg gacgacttgc
     5641 gcgggtcctt tgccgtgaag cgcattgccg ttggacacgc cgtcgaagat ggcgtttggg
     5701 cgaaggacgg cgtgttgctc gaaatggaag gcgggcaaga ggtcggccgc gccgatctcg
     5761 ccggcatcgg gtctttgcgc ggcgcgcata attggcagaa cgccgccgcc gcctacgcgc
     5821 tcgctcgcgc catgggactg gacggcgctg ccattcagca aggcttgaag agtttcgtcg
     5881 gccttgccca tcgcatggaa caggtggcgc gcgtgggcaa tgtgctgttc gtcaatgact
     5941 caaaggcaac gaacgcggat gccgccggca aggcgctggg cagcttcgac gacatctatt
     6001 ggattgtcgg tggccgcgcc aaggatggcg ggcttgccgg gcttgaagcg ttctatccca
     6061 aaattacgcg cgcctatttg atcggcgagg cggcggagga cttcgcgcat cagctgggcg
     6121 atgccgtacc tcatgtcgtc tgcggtaccc tcgaccgcgc cgtgacggac gccgcgcgcg
     6181 atgcggg
//
LOCUS       DBNHVU010000146         6474 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_127003, whole genome shotgun sequence.
ACCESSION   DBNHVU010000146 DBNHVU010000000
VERSION     DBNHVU010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6474)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_127003"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="AC16Y7_05880"
     CDS             complement(<1..185)
                     /locus_tag="AC16Y7_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708944.1"
                     /translation="MGVVTARLVFLAFLGLTGTIIYNALYLQEHAPRGMSSADASTQG
                     VATISVVPKDEPPEFDAP"
     gene            complement(293..2086)
                     /locus_tag="AC16Y7_05885"
     CDS             complement(293..2086)
                     /locus_tag="AC16Y7_05885"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR5708945.1"
                     /translation="MRVFGNESFRGLSVYFGSLVHNSARGDALTIARHRSFIASRLLG
                     GLLALCVFPIYLVVGGKPSLFGAAAFLWLLSPIAIAIFLSRTGRFGAAHLISAVNFAG
                     LVTYCAWLTGGLASGLLPWLVAVPFEAGLATDRRIVIWAGGASAFGLVVLAVLGAAGL
                     VPASALPLSPSVFALLGAASATAYAAGLGVMSQVVHRHSESAIRESEERYRLIAENAN
                     DMITSHDAEGRVVFASLAAQNLLGEPVQKILGDGLFERVHLADRPAYLTALSRCQASN
                     QTIAVEFRVRRPGAGEGDRYAWVEMRCRPMPSVGGAGQERSAVVAVTRDITEHKAQEA
                     QLLKARDEAESASRAKTQFLASMSHELRTPLNAIIGFSEMLNKELYGKLGDARYRDYS
                     RLIHESGEHLLNVVNDILDMSKIEAGKFKIVKEPLDVAALVTSCCDVMRHMAEQKQID
                     LKVDVGDVPALAADKRACKQMLLNLISNAIKFTEPGGWVKVTAHIKDGSIAFAVSDNG
                     IGIADEDLARLGKPFVQASGSYDRNYEGAGLGLSVVKGLARLHDGALEITSTLGKGAT
                     ATITLPMEAESELAEPYPAESCPHVPTASAA"
     gene            2472..2924
                     /locus_tag="AC16Y7_05890"
     CDS             2472..2924
                     /locus_tag="AC16Y7_05890"
                     /inference="COORDINATES: protein motif:HMM:NF036461.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5330 domain-containing protein"
                     /protein_id="HIR5708946.1"
                     /translation="MMFLIRTGFWLLVLVLLLPTDENQQNEIYGTAQTAMNDVATFCD
                     RNPQTCVKGQDAFELLVQKAQYGAQVVMNLVEEQTGNLDSNPVPVPGPVPGPVPMPAD
                     NNVMPSDNSVMPVPAAAPLEPATTWDASASQDTLNPQDREAAWGGPGI"
     gene            3136..3573
                     /locus_tag="AC16Y7_05895"
     CDS             3136..3573
                     /locus_tag="AC16Y7_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HIR5708947.1"
                     /translation="MDTATSTSVPTFDEILADFELLDEWEDRYRYVIELGRKLRPLPD
                     EERNAQNKVEGCVSQVWLSTTIERDGAPRLTFIGDSDAHIVRGLIAILFAIYSGKSAD
                     EILSIDAVKTLGDLHLKEHLTPQRSNGLMAMVKRIQNDAATAN"
     gene            complement(3632..4147)
                     /locus_tag="AC16Y7_05900"
     CDS             complement(3632..4147)
                     /locus_tag="AC16Y7_05900"
                     /inference="COORDINATES: protein motif:HMM:NF018960.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR5708948.1"
                     /translation="MQGQRELYDYWRQSAGERVMPARPDFDPLKVPGLLPHIGLSDVR
                     DGLDKGSFTLAGTCLRDIYGAEITGRRLSDIFCGDCATYWSRVYARIAMAGLPGTGVV
                     RGPSKGRDHVVLFWLRLPQSDDGRRVDRIHCLDIAGPFETNEADTPCASQCYPRVSPE
                     PVTLGARVAVA"
     gene            complement(4396..4803)
                     /locus_tag="AC16Y7_05905"
     CDS             complement(4396..4803)
                     /locus_tag="AC16Y7_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ros/MucR family transcriptional regulator"
                     /protein_id="HIR5708949.1"
                     /translation="MDANLEKNELVELTAEIVSAYVSNNTVVAADLPGVIQNVFEALS
                     KASNASMQPPKEELKPAVPIKKSVTPEFIICLEDGKKFKSLKRHLRTHYDLSPEEYRE
                     KWGLTHDYPMVAPNYAAARSDLAKRMGLGQRRR"
     gene            5248..5697
                     /gene="msrB"
                     /locus_tag="AC16Y7_05910"
     CDS             5248..5697
                     /gene="msrB"
                     /locus_tag="AC16Y7_05910"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIR5708950.1"
                     /translation="MTKAEKNQNTAKEQHAVSKEEWRDKLSAEQYHVTREHGTERAFS
                     HPHHAEKRDGMYRCVCCGTALFSSDTKFDSGTGWPSFTDVASADTVAKHEDSSFGMRR
                     VEVRCANCDAHLGHVFPDGPGPTGMRYCINGCALDLDTSDASGETSS"
     gene            5723..>6474
                     /locus_tag="AC16Y7_05915"
     CDS             5723..>6474
                     /locus_tag="AC16Y7_05915"
                     /inference="COORDINATES: protein motif:HMM:NF014896.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR5708951.1"
                     /translation="MNETAPLACAPCAERRPSRSAHHGIEREDDYAWLRADNWQAVMR
                     DPDALPADIRAHLEAENAYTGAVMADTESLQEALFAEMKGRIKEDDSSVPAPDGAFDY
                     YARYVVGGQHPLYCRQPRGGGEEEILVDGNTLAEGHAYFHIANVAHSPDHKLIAYAVD
                     TKGSEFYTVKIVDAETHAEVDSSIADNNGSFQWAADSKTLLYIWLDEEHRPRKLFAHG
                     IGLEGDDHLIHEQADPGYFLEIGTTQDNKYLLL"
BASE COUNT         1381 a         1996 c         1886 g         1211 t
ORIGIN      
        1 ggcgcatcga attccggtgg ctcgtccttt ggcacgacgg agattgtcgc aacgccttgc
       61 gtggacgcat ccgcgctgct catccctcgg ggcgcatgct cctgaaggta gagtgcgttg
      121 tagatgatcg tcccggtcag gcctaggaag gccagaaaca cgagccgcgc tgtgactacg
      181 cccatcgggt cctacattcg ttacaaatca tggcaagaga ggctcgccgc ctctctttgg
      241 ggcatcttgg cgtccgaaac taaagagttg ctaaacaacc ccgctgggtg cctcacgccg
      301 ccgatgccgt cggcacatgg gggcaagatt ctgcgggata aggttctgcc aactctgact
      361 ctgcctccat cggcaaggtg atggtcgccg tcgcaccctt ccctagcgtg cttgtgatct
      421 caagcgcacc gtcgtggagg cgcgcaagtc ctttcacaac agatagtcca agccccgcgc
      481 cctcgtagtt cctgtcatag gagccactgg cctgcacgaa tggcttgccg agccgcgcca
      541 ggtcttcgtc ggcgatgccg ataccgttgt cggagacggc gaaggcgatg cttccgtcct
      601 taatatgtgc cgtgaccttc acccatccgc caggctcggt gaacttgatc gcgttcgaaa
      661 tcaagttgag cagcatttgc ttgcacgcgc gcttgtcggc cgcaagcgcg gggacgtccc
      721 cgacatcgac tttgagatcg atctgcttct gctccgccat gtgacgcatg acatcgcagc
      781 aggacgtcac aagcgcggcg acatcgagcg gttccttcac gatcttgaac tttccggcct
      841 cgatcttcga catgtcgaga atgtcgttga ccacattgag aaggtgctcg ccgctctcat
      901 ggattaggcg tgaataatcg cgatagcgcg catcgccgag cttcccgtaa agttccttat
      961 tgagcatctc cgaaaagccg atgatcgcat tgagcggcgt acgcagttca tgactcatgc
     1021 tcgcaaggaa ctgggtcttg gcccggcttg cgctttcggc ctcgtcccga gccttgagaa
     1081 gctgcgcttc ttgcgccttg tgttcggtga tgtcgcgtgt cacggccacg acggccgacc
     1141 gttcctgacc ggcgcctccg actgagggca ttgggcggca ccgcatctcg acccatgcat
     1201 agcggtcgcc ctcgccggcc ccagggcggc gcacgcgaaa ctccacggca atggtctggt
     1261 tactggcctg gcagcgcgac aatgctgtca gatatgcggg ccggtccgcc aagtgcacgc
     1321 gctcaaacag accgtcgccc agaattttct gaaccggctc accgagcagg ttctgggctg
     1381 ccagcgaggc gaaaacgacg cggccttcgg catcgtggct cgtgatcatg tcgttcgcgt
     1441 tctcggcaat gaggcgatag cgctcctcgc tctcccgaat ggcgctttcc gaatgtctgt
     1501 gcaccacttg gctcatcacc cccagccctg cggcgtaagc ggtggcagat gctgcgccga
     1561 gcagcgcgaa gacagatggc gatagcggta atgccgaggc ggggacgaga ccggcagcgc
     1621 ccaagaccgc caacacgacg agcccaaagg ccgatgcccc acccgcccaa atgacgatcc
     1681 gcctgtccgt ggcaagcccg gcctcgaaag gtaccgcaac cagccatggc agcaggccgg
     1741 aggcgaggcc gcctgtcagc catgcgcaat aggtcacgag gcctgcgaaa ttgacggccg
     1801 atatcagatg tgccgcccca aaacggccgg tgcgcgaaag aaagatggcg atggcgatgg
     1861 gcgacagcag ccataggaag gcggcggcgc caaaaagcga cggttttccc cccacgacga
     1921 ggtagatggg aaacacacag agcgccagga gaccgccaag caggcgcgag gcgatgaacg
     1981 accggtgccg cgctatcgtg agggcatcgc cgcgcgcgga attgtgcacc agcgagccga
     2041 aatacaccga cagcccgcga aaactctcat taccaaacac gcgcatctcg tagcggccca
     2101 gtggccgctt cgtccccgat tgaatcgaac cgggattatt cgggggccac gcttaaggaa
     2161 tgcataaagc gcgggggccc ccgggcgctg agcgaagctc cggaaccgca aacggggccg
     2221 tggaaaccac atcgtaaagc agtccttgcc ggcacatttc caaattgaga taattttgag
     2281 aggaaataaa aatgaagtat aaagaagtac taattgcatt agagtcttcg ttgagtgaag
     2341 ttgtcgattc aaaatttagg ggtttccgag acggtatatt attttcttcg agctatagtg
     2401 cgtcaatctg caggggaaat tgcagaaaaa catacggcaa aaaagccgta gttaacagag
     2461 agggcaaatc gatgatgttt ctaatcagaa cgggcttctg gttgctcgtt cttgttctgc
     2521 tgcttccgac cgacgagaac cagcagaatg agatctacgg gaccgcgcag acggccatga
     2581 acgacgttgc gacgttctgc gaccgcaatc cgcagacatg cgtcaagggt caggatgcct
     2641 ttgaactgct ggtgcaaaag gcccagtacg gcgcccaggt cgtgatgaac ctggtcgaag
     2701 agcagacagg gaacctcgac agcaaccctg tcccagttcc cggacctgtc cctggaccgg
     2761 tgccgatgcc agccgacaat aatgtcatgc cgagcgacaa cagtgtcatg ccggtcccgg
     2821 cagcggcgcc cttggagccg gcgaccactt gggatgcatc tgcgtcccaa gacaccttga
     2881 atcctcagga ccgggaagcc gcttggggcg gtccgggcat ctaaagggca gtgcgccggc
     2941 tttcgcgccg gcgcgaattg agaattgtcc gattgcgcgg tgcgatcgga gtccaagacc
     3001 tgttggcagc cccccgcctg ccaaacgagg gcgcgcagtt gggaaggttt ctgccctttc
     3061 cctcccaaac tgcgcgccct atttcttttg acaaaggccg tcgcggccgg gctcatcccc
     3121 ccctatattt gggatatgga cactgcaacc agcacctctg ttccgacctt cgacgagatc
     3181 cttgccgact tcgagctgct cgatgagtgg gaggatcgct accgttacgt cattgagctg
     3241 ggccgaaagt tgaggccgct gcccgatgaa gagcgcaacg cgcagaacaa agtcgaaggc
     3301 tgcgtcagcc aggtctggct ctccaccacg atcgagcgcg atggcgcgcc gcgcctcacc
     3361 ttcatcggcg acagcgacgc gcatatcgtg cgcggcctga tcgcgattct gtttgccatc
     3421 tactcaggca agagcgcgga cgagatcttg agcatcgatg ccgtcaagac actcggcgac
     3481 cttcacctca aggagcatct gacgccgcag cgctccaatg ggctcatggc gatggtgaag
     3541 cgtattcaga acgacgccgc cacggcgaac taggcttact cagcgcctac aagggcgagc
     3601 tcgcgggcct tgaatgcggc caatcgcacg gtcaggcaac agctacgcgc gccccaagcg
     3661 tgaccggctc cggcgacacc cggggatagc actgagatgc acagggggtg tcggcctcgt
     3721 tcgtttcaaa cgggccagcg atgtcgagac agtggatccg atccacgcgg cgcccatcgt
     3781 ccgactgggg caaccgcaac cagaacagca cgacatggtc gcggcccttc gaaggcccgc
     3841 gaacgacgcc ggttccgggc aagcctgcca tggcgatgcg cgcgtagaca cggctccaat
     3901 acgtcgcgca atccccgcag aagatatccg acaggcgtcg cccggtgatt tccgcgccat
     3961 aaatatcgcg caggcacgtg cctgcgagcg tgaacgagcc tttatccagt ccgtctctga
     4021 catcgctcag accgatatga ggcaagaggc cgggcacctt aagcggatcg aaatcgggcc
     4081 gcgccggcat gacccgctca ccagcgcttt ggcgccaata atcgtagagt tcgcgttgtc
     4141 cctgcatgac cagctgggcg cgaaaagcgc atgcggacga cattgttgtt tccccctgcc
     4201 gcgcagcgct ttgcttttgc gcgaatcgcg accccacgac tatgtggagt tcgcctgtca
     4261 catacaaggg atgcaggacg gattcagcta agagtcacaa tggcttgtag atagagcaat
     4321 ggcggccacc cgatcttgcg ggcgccgccg tttcaaaaat cgtaagcgct ggagtcgctt
     4381 aagcctgccg cgttcctagc gccgccgctg accaagcccc atgcgcttgg cgagatccga
     4441 acgcgctgcc gcgtagttcg gcgcgaccat gggatagtcg tgagtcaagc cccacttctc
     4501 ccggtattct tcgggcgaca aatcgtaatg cgtgcgcaga tggcgcttca gcgatttgaa
     4561 cttcttcccg tcctcgagac agatgatgaa ttcgggcgtc acggacttct tgatcggaac
     4621 cgcaggcttc aactcttcct tcggcggctg catagaagca ttggacgctt tgctcagcgc
     4681 ttcgaacaca ttttggatga cgccaggtag gtcggcggcc acgacagtgt tgttgctgac
     4741 ataagccgag acgatctcgg cggtcagctc aacgagttcg ttcttctcca aattcgcgtc
     4801 catcaaattc gctccttgat ccgcctaatg tccggctttg gccgagcggg ccgcaatttg
     4861 gcggtcccta gcacatctcg ccagctctca gggctccttg agggccagaa acttcgatgt
     4921 tacacaacta tccagcatgt aaatcaataa catgtctctc tgatctgtct ctatagcact
     4981 tttccacaaa cttgcaacgc aactcttctg acgaagactg cattctaacc agtggactaa
     5041 agggcttgga gatcttccca ggcgaacgag attcaaacgc tgacgcaggt cagtcgaaag
     5101 cacggcgttg cgatgcagcc ctcagaagag acggctcgcc tgccaatcaa gcctgcgtga
     5161 gagcgatggg agacctcttc gggacgtgtc ccgaagtact tatattctgc gtgacctccc
     5221 gcagacgaga tcgagagcct cacaagaatg accaaagctg agaagaacca gaacacggcc
     5281 aaagagcaac acgctgtctc taaagaagag tggcgcgata agctctcggc ggagcagtat
     5341 cacgtcaccc gtgaacacgg caccgagcgc gcgttcagcc acccccacca tgcggagaag
     5401 cgcgacggca tgtatcgctg cgtatgctgc gggacggccc tcttcagctc ggacacgaag
     5461 tttgattccg gcaccggctg gccaagcttc accgacgtgg cgtccgcgga caccgtcgcc
     5521 aagcacgagg acagctcctt cggcatgcgg cgcgttgagg tgcgttgtgc aaactgcgac
     5581 gcgcatctcg ggcacgtgtt ccccgacggc cctgggccga ccggcatgcg ctattgcatc
     5641 aacggttgcg ccctcgacct tgacaccagc gatgccagtg gcgagacctc aagctagtct
     5701 tccgtctaca caataaagcg acatgaacga aacagctccc ttggcgtgcg caccgtgcgc
     5761 cgaacgccgc ccatcgcgct ccgcccatca cggcatcgaa cgtgaggacg attacgcttg
     5821 gctgcgtgcc gacaactggc aagcggtcat gcgagatcca gacgcgctgc ctgcggacat
     5881 tcgcgcgcat ctcgaagcgg agaacgctta tacgggcgcc gtcatggcgg acaccgagag
     5941 ccttcaggag gcgttgttcg ccgagatgaa ggggcgcatc aaggaggacg actcaagcgt
     6001 cccggcaccc gacggcgcct ttgactacta cgcccgctat gtcgtgggcg ggcagcatcc
     6061 cctttactgc cggcagccac gcggcggcgg tgaagaagag atcctggtcg acggcaatac
     6121 gttggccgaa ggccatgcct atttccacat cgccaacgtc gcccacagcc ccgaccacaa
     6181 acttatcgcc tacgcggtcg atacgaaggg ctcggagttt tacaccgtca agatcgtcga
     6241 cgcggagacg cacgccgagg tcgatagctc tatcgccgac aacaatggca gtttccaatg
     6301 ggccgccgac agcaagacgc tcctctatat ctggctcgac gaggagcatc ggccccgcaa
     6361 acttttcgcg catgggatcg gcttggaagg cgacgaccat ctcatccacg agcaagccga
     6421 ccctggctat ttcctcgaga tcggcaccac tcaggacaac aagtacctgt tgct
//
LOCUS       DBNHVU010000147         7659 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1764810, whole genome shotgun sequence.
ACCESSION   DBNHVU010000147 DBNHVU010000000
VERSION     DBNHVU010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7659)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7659)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7659
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1764810"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1289
                     /gene="ftsW"
                     /locus_tag="AC16Y7_05920"
     CDS             111..1289
                     /gene="ftsW"
                     /locus_tag="AC16Y7_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HIR5708952.1"
                     /translation="MRITRADKSILSDWWFTVDRLMFFGLLLLMGAGLVLSLAASPPI
                     AEKLDLESFYFVRRHAMMLLPAVAVMFAASTLTPKQIRRTSLVIFFIGIALMIGILFF
                     GPEVKGAKRWLQVGAFSLQPSEFVKPAFIVLTAWLFNESQKRKDVPSLEFAIALYAVF
                     AVLLMLQPDFGQTLLVSLVWGALLYMAGINMLWIAALIALGAAGVVAAYLLLPHVASR
                     IDRHIDPASGDTYQSDRSLDSFLHGGWLGRGPGEGTVKDVLPDSHTDFIFAVAAEEYG
                     LIACLILLTLFAFIVLRGLSKASQEPDGFIRHAVAGLVMLFGLQTLINMAVNVGLLPA
                     KGMTLPFISYGGTSLLAMALTMGFALGLTRKRPMAGRLKHSARERHGAQTAGARGQTA
                     "
     gene            1286..2386
                     /gene="murG"
                     /locus_tag="AC16Y7_05925"
     CDS             1286..2386
                     /gene="murG"
                     /locus_tag="AC16Y7_05925"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008547994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR5708953.1"
                     /translation="MTQTILLAAGGTGGHLFPATALAQELTRRGFAVELATDERAEQY
                     GTDFPARAIYRVPSATFTSRAPLAVAKTLGRLGKGFLRARRLLEMVQPHVVVGFGGYP
                     TVPPIMAARSLAIPTVIHEQNAVMGRANRMLSRFADAIALSFPSTKYLRRSAQKRSIV
                     SGNPVRDEVISYRDVPYAPPEPAGRLLVLVFGGSQGARFFSEVLPPAMEMMPSAARWR
                     LTVVQQARPEDLNELRAAYKEANIAAHVAPFFRDLPERIANAHLVISRAGASTVAELM
                     AIGRPALLVPLPHALDNDQLENATRLEESGGGWCMPQSGITPENLSEELARLLGAPDM
                     LAKTAARSKAMANVDAVKKLANLVEELAGPEE"
     gene            2401..3825
                     /gene="murC"
                     /locus_tag="AC16Y7_05930"
     CDS             2401..3825
                     /gene="murC"
                     /locus_tag="AC16Y7_05930"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR5708954.1"
                     /translation="MPPPQTTGLFHIVGIGGIGMSAIAEVMHTRGYRVQGSDLKEGAN
                     LQRLRDHGIACLIGHDPGNIEGASYLVISSAVKSGNPEFEAAKERGIPIIRRAEMLAE
                     LMRPCTTVAVTGTHGKTTTTSIIADLLQGGGCDPTVITGGIINAWGTNARIGQGEWMV
                     VEADESDGTFLKLPTEIGVITNIDPEHMDFWESEEVLYEAFEQFIEAIPFYGVVVACI
                     DHPVVRGLMAALGGAENGRRTLTYGVSRSADIRLFNVRPDHATMTFDVRLGSGVAGGA
                     RELSDLTLPTLGHYNALNAIAALAVATEAGVSDEVIRTTLAGFSGVKRRFTRVGAWQG
                     IAIYDDYAHHPVEIAAVLRGASAAGNGRIIAVMQPHRYTRLQSLFNEFSACLDDADIV
                     ILTPVYSAGEPPIPGIDRDELAAGLRRHGHPNVFMVDDEESLTAAVASVAKDGDLVVG
                     LGAGTITDWINALPAQLKQHGGKA"
     gene            3822..4733
                     /gene="murB"
                     /locus_tag="AC16Y7_05935"
     CDS             3822..4733
                     /gene="murB"
                     /locus_tag="AC16Y7_05935"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR5708955.1"
                     /translation="MSLVETLTASMPDLRGRLRGDAPLKDYTWFRVGGPAEVLYSPAD
                     EADLAYFLKTLPADTPITMIGLGSNLLVRDGGIEGVVMRLGRGFGEIKVEDGHRLRVG
                     TAVPDVRVARAAADAGIAGLSFYRGIPGGVGGALRMNGGAHGSETCEVLIEARAVDRD
                     GNIHVLPVEDFHYSYRHCGVPDDYIFTEALFQGEPGDPEKILAEMDDIAAYREEVQPI
                     KSRTGGSTFKNPPGNKSWQLIDAAGCRGLTIGDAKVSEMHCNFLINEGEATAADIEML
                     GETVRARVKETSGVELEWEIKRLGKPA"
     gene            4882..5817
                     /locus_tag="AC16Y7_05940"
     CDS             4882..5817
                     /locus_tag="AC16Y7_05940"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HIR5708956.1"
                     /translation="MTKRQTHVAVLMGGLSSEREISLRSGEACAEALEGEGYKVTKVD
                     VGQDVAARLAEIAPDVCFNALHGRYGEDGCIQGVLEYLGLPYTHSGVLASALAMHKER
                     AKAALREAGVPVAESRVVARAEAAKGHVIAPPYVIKPPAEGSSVGVYIVREDQEHPPQ
                     ELNSADWALGEELMVERFIPGRELTCAVMGEEALGVIEIRPADGLAFYDYESKYAPGG
                     SIHVLPAPLKPNVYQLVQKLSVAAHRALGCRGVSRSDFRFDDRPAGTGELICLEINTQ
                     PGMTSTSLVPELAAYGGRSFGELVRWIVEDASCNR"
     gene            5805..6800
                     /locus_tag="AC16Y7_05945"
     CDS             5805..6800
                     /locus_tag="AC16Y7_05945"
                     /inference="COORDINATES: protein motif:HMM:NF015739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR5708957.1"
                     /translation="MQSLGPDRGHARGRRSQEALLARGIAAPRDASRRLPALSGTRAL
                     IGLKPRSLRLGRTGRDPVRGKWLLLSALFLASAVVYGAIIGGQTGVLFNYAVAGVEHV
                     AVAVGFGVKRVSVEGQQNATDSAITAALGAGPQTLMLAFDTDAAKARLEAVPWIRHAR
                     VMRLLPSTLQVMIEEREPYAVWQNKGKTFVVDDQGVVLAPALPQAYPDLPLVVGEGAS
                     EHAAALYATLQSYDQLKQKMLAALRVGDRRWTLKLRTGTEIMLPDDNIEVALQSLTEL
                     QQEHGVLERDWSVIDLRLLDRITVRTREAAIVPPDDIAPSDVPTAATGTTPKGKT"
     gene            6820..>7659
                     /gene="ftsA"
                     /locus_tag="AC16Y7_05950"
     CDS             6820..>7659
                     /gene="ftsA"
                     /locus_tag="AC16Y7_05950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR5708958.1"
                     /translation="MTYSSTIRSKRQRIVTVLDIGTSKVCCLIGKTSAAPDWFEGDGD
                     AVHFEVLGFDHTRAEGLKSGMVTHLDSAEQCIRAAVDAAERMAGVVVEDVHVSVTAGR
                     LKSESFSASVALQSGAVREDDVHRLLAGGRQYAARDKRTVLHALPTGYRLDENGGISE
                     PHGMCGERLSVDLHTVTADDVAMRNLILCVERCHLGVAGLVAAPYSSALSVVTPDEAK
                     LGVACIDFGAGTTTLSVIADGHFIHADAIALGGNGITTDIARTLGAPMEHAERLKTLH
                     GSAF"
BASE COUNT         1293 a         2466 c         2484 g         1416 t
ORIGIN      
        1 tctgcctctt tcgatcagtt cgataacttc gccaagcggg gcgatgcatt ccgtgagctt
       61 gtcttaggac tcgacggcgc gacgagcgtg acgcagcgag ggaaggcggc atgaggatca
      121 cgcgcgccga taagagcatc ctctccgact ggtggttcac cgtcgaccgc ctgatgttct
      181 ttggccttct cttgctcatg ggcgccgggc ttgtcttgtc cttggctgcc agcccgccga
      241 ttgccgagaa gctcgacctg gagtcgttct acttcgtgcg gcgtcacgcc atgatgctcc
      301 tgcccgcggt cgccgtcatg tttgccgcct caacgctgac gccgaagcag atcaggcgca
      361 ccagtctcgt gatcttcttt atcggcatcg ctctgatgat cggcattctc ttcttcgggc
      421 ccgaggtgaa gggcgccaag cgctggctcc aggtcggcgc gttttcgctg cagccgtcgg
      481 agttcgtgaa gccggccttc atcgtgctca ccgcgtggct cttcaacgaa agccagaagc
      541 gcaaggacgt gcccagtctc gagtttgcaa tcgcgcttta cgccgtcttc gctgtgctct
      601 tgatgctcca gccggacttt ggccagacgc tcctcgtcag tctggtgtgg ggcgcgcttc
      661 tctatatggc gggcatcaat atgctctgga ttgccgccct gatcgcgctc ggcgccgcgg
      721 gcgtcgtggc ggcatatctc ctcctgccgc atgtggcctc gcgcatcgac cgccatatcg
      781 atccggcctc cggcgacacc taccaatcgg accgatcgct ggattccttc ctccacgggg
      841 gctggctggg gcggggaccc ggcgagggga ccgtaaaaga cgtcctgccg gactcccata
      901 cggacttcat ctttgccgtc gccgccgaag agtatggctt gattgcttgc ctgattttgc
      961 tgactctatt cgccttcatt gtgctgcgtg gtctgtccaa ggcatctcaa gagcctgacg
     1021 ggttcatccg gcacgccgtg gcaggactcg tgatgttgtt tggcttgcag accttgatca
     1081 atatggccgt gaatgtcggc cttctcccgg ccaagggcat gaccttgccg ttcatctctt
     1141 atggcggtac gtcgctcctc gccatggcgt tgaccatggg gttcgcgctg gggctcaccc
     1201 ggaaacggcc catggccgga cggctgaagc attcggcgcg cgaacggcac ggggcgcaga
     1261 ccgcaggggc gagggggcag acggcttgac tcagaccatt cttcttgccg ctggcggcac
     1321 gggaggacat ctctttcccg ccacggcctt ggcccaggag ctgacgcgcc gcggtttcgc
     1381 cgtggagctc gcgaccgatg agcgcgccga gcagtacggc acggattttc cagctcgcgc
     1441 catctaccgg gtcccatcgg cgaccttcac gagccgcgca ccgcttgccg tcgcgaagac
     1501 gctcggccgt ttgggcaaag gcttcctacg cgcccggcgg ctcctggaaa tggtgcagcc
     1561 gcacgtggtc gtgggcttcg gtggctatcc cacggtcccg cccatcatgg cggcgcggtc
     1621 gctggccatt cccaccgtca tccacgagca gaacgccgtc atggggcgcg ccaaccgcat
     1681 gttgtcccgc ttcgccgacg ccatcgcgct gtcgttcccg tcgacaaaat atttgcgccg
     1741 cagcgcgcag aagcgctcga tcgtctcagg caatccggtg cgcgatgagg tgatctcata
     1801 tcgcgacgtt ccctacgcgc cgcccgaacc ggcagggcgg cttctggtcc tggtgttcgg
     1861 cggcagccaa ggcgcgcgct tcttttccga ggtcttgccg ccggcgatgg agatgatgcc
     1921 ctcagcagcg cgctggcgtc tgacggtcgt gcagcaggcc cggccggagg acctcaacga
     1981 actccgcgcc gcctacaagg aagccaatat tgccgcgcac gtggcgccgt tcttccgcga
     2041 tctgcccgag cgcatcgcca acgcccatct ggtgatctcc cgtgccgggg cctcgaccgt
     2101 ggccgagctg atggccattg gccgtccggc cctgctggtg ccgctgcccc acgccctcga
     2161 caacgatcag ttggagaacg caacgcgtct tgaggaaagc ggcggcgggt ggtgcatgcc
     2221 ccaatcgggc atcacgccgg agaatctgtc ggaggaactc gcccggcttc ttggcgcgcc
     2281 ggacatgttg gccaagacgg cagcgaggtc caaggccatg gccaatgtcg atgcagtcaa
     2341 gaaactcgcc aacctcgtcg aggagctggc ggggcccgaa gaataggcgc atcgatgcat
     2401 ctgcccccgc cacaaacgac aggcctgttc cacatcgtgg gcattggcgg tatcggcatg
     2461 agcgccatcg ccgaggtgat gcatacgcgc ggctaccgcg tacaaggcag cgacctgaaa
     2521 gagggcgcca accttcagcg cttgcgcgat cacggcatcg cctgcctcat cggtcatgat
     2581 ccgggcaata ttgaaggcgc ctcgtatctg gtgatctcca gtgccgtgaa gtcggggaac
     2641 ccggaattcg aagcggccaa ggagcgcggt attccgatca ttcgccgtgc ggagatgctc
     2701 gctgagctca tgcgcccatg cacgacggtg gcggtgacgg gcacgcacgg caagaccacg
     2761 acgacatcca tcatcgcgga cctattgcag ggcggcggct gcgatccaac cgtcatcacg
     2821 ggcggcatta tcaatgcctg gggcaccaac gcgcgcatcg gacaaggcga atggatggtg
     2881 gtcgaagccg acgagtccga cggcacgttt ctaaaactcc cgacagagat cggcgtcatt
     2941 accaacatcg atcccgagca catggacttt tgggagtccg aagaggtgct ctatgaggcc
     3001 ttcgagcagt tcatcgaggc gatccccttt tacggtgtcg ttgtcgcatg catcgaccat
     3061 cccgtcgtac gcggtcttat ggctgctctg ggcggcgcag aaaatgggcg gcgcacattg
     3121 acctatggcg tgtcgcgctc ggccgacatt cggctgttca atgtgcgccc cgaccacgcc
     3181 accatgacat tcgatgttcg cctgggctca ggtgttgcag gcggtgcccg cgaactcagc
     3241 gatctgacgc tgccgactct gggccactac aacgcgctca atgcgattgc cgcgttggcg
     3301 gtggccacgg aagcgggcgt gagcgacgag gtgatccgca cgacgcttgc tggcttctcc
     3361 ggggtgaagc ggcgctttac ccgcgtcggc gcgtggcaag gcatcgccat ctatgacgac
     3421 tacgcccatc acccggtgga gatcgccgct gtcctgcgcg gggcgagtgc ggcaggcaat
     3481 ggccgcatca tcgcggtcat gcagccgcat cgctatacgc ggctccagtc gttgttcaac
     3541 gagttctccg cctgcctcga cgacgccgac atcgtgatcc tgacgccggt ctactccgcg
     3601 ggcgagccgc cgatccccgg tatcgaccgc gacgaactgg cggcggggct ccgccgtcac
     3661 ggccacccca atgtgttcat ggtcgacgac gaagagtcgc tgacggccgc cgtcgcctcg
     3721 gtcgctaagg acggcgacct cgtcgtgggg ctcggcgccg gcactatcac cgattggatc
     3781 aacgcacttc ccgcccagct gaagcagcac gggggtaagg cgtgagcttg gtcgagacgc
     3841 tgacggcgtc catgccggac ctgcgtggcc ggctcagagg cgatgcgccg ctgaaggact
     3901 acacttggtt ccgcgtgggc ggcccggccg aagtgcttta ttcgcccgcc gacgaagcgg
     3961 accttgccta tttcttgaag acactccctg cggacacacc catcaccatg attgggctgg
     4021 gctcgaacct gctggtacgc gacggcggga tcgagggcgt ggtcatgcgc cttggccgcg
     4081 gcttcggcga gatcaaggtc gaggatggcc atcgcttgcg cgtcggcacg gcggtgcccg
     4141 acgtcagggt ggcgcgcgcg gccgccgatg cgggcattgc cgggctgtcg ttctatcgcg
     4201 gcattccggg cggcgttggt ggcgccttgc gcatgaatgg cggggcgcat gggagcgaga
     4261 cctgcgaggt gctcatcgag gcacgcgccg tggaccgcga tggcaacatc catgtgctgc
     4321 cggtcgagga cttccactac agctaccgcc attgcggtgt gcccgacgac tacatcttca
     4381 ccgaggcgct gttccaaggt gagcccggcg accctgagaa gattcttgcg gagatggacg
     4441 acatcgccgc ctaccgcgaa gaggtgcagc ccattaagag ccgcacgggc ggatccacct
     4501 tcaagaaccc gcctggcaac aagtcgtggc agctcatcga tgccgccggc tgccgggggc
     4561 tgacaatagg ggacgcgaag gtctccgaga tgcactgcaa cttcctcatc aacgagggag
     4621 aggccacggc cgccgacatc gaaatgctcg gcgagacggt gcgcgcgcgc gtcaaggaga
     4681 cgagcggcgt cgagcttgag tgggaaatca agcgcctcgg caagcccgcc tgaagccgca
     4741 gtttttctgc cgcctccctc gtgccattcg gcgcgaatac aggtaaccag ggtctcaagt
     4801 gatttggtcc caagtgattt gcgcgtccgg cgcggggtcg ggcgcgtttg tgcacgccaa
     4861 ctaagcaatg ggggctttgg catgacgaag aggcaaaccc acgtcgccgt gctcatgggc
     4921 gggctgtcgt cggaacggga gatctcctta cgctctggcg aagcgtgcgc cgaggccctg
     4981 gagggcgagg gctacaaggt caccaaagtc gatgtcggcc aagacgtggc cgcgcggctt
     5041 gccgagatcg cgcctgacgt ctgcttcaac gcgctccatg gccgttatgg cgaggatggc
     5101 tgcatccagg gcgtcctgga gtatttgggg ctgccctaca cgcattccgg cgtgctggcc
     5161 tcagcacttg ccatgcacaa ggaacgcgcc aaggcggcct tgcgcgaggc cggcgtgccg
     5221 gtggccgaaa gccgcgtggt tgcccgtgcc gaagccgcca aagggcacgt cattgcgccg
     5281 ccctacgtga tcaagccccc ggccgaaggc tcgtccgtgg gcgtctacat cgtccgcgag
     5341 gaccaggagc accccccgca agagctcaat tccgccgatt gggccctcgg cgaggaactc
     5401 atggtcgagc ggttcatccc cggccgcgag ctcacctgtg ccgtgatggg cgaggaggcc
     5461 ttgggcgtca tcgaaatccg ccctgcggac gggctcgcct tctacgacta tgagtcgaaa
     5521 tacgccccag gaggctcaat tcatgtgctc ccggccccgc ttaaaccaaa tgtttaccaa
     5581 ttggtacaga agctgtcggt agcggctcat agggcactgg gctgtcgtgg ggttagccgg
     5641 tccgactttc gattcgacga ccggccggcc ggcacggggg agctgatctg cctagagatc
     5701 aacacccaac ctggcatgac ttccacgtct ctggtgcccg agctcgcggc ctatggcggc
     5761 cgctcttttg gagagttggt gcgctggatc gtggaggacg cgtcgtgcaa tcgttaggcc
     5821 cggatagggg tcacgccaga ggaagacggt cgcaagaggc cctgctcgcg cgcggtatcg
     5881 ccgcgccgcg ggacgcttca cgccgccttc cggctctgag cggtacgaga gccctgatcg
     5941 gtctgaagcc caggagcctg cgcctcggcc gcaccggccg cgaccccgtc cgcgggaagt
     6001 ggctgcttct gagtgcccta tttcttgcca gtgcggtcgt ctatggcgcc attatcggcg
     6061 gccagacggg cgtgctgttc aactatgcgg tggccggcgt cgaacacgtt gcggtggccg
     6121 tgggcttcgg cgtcaagcgc gtcagcgtcg aaggtcagca aaacgccacg gactcggcca
     6181 ttaccgccgc acttggcgcc ggtccgcaga ccctaatgct cgccttcgac accgacgccg
     6241 ccaaggcgcg tttggaggcc gtaccgtgga tccggcatgc gcgggtcatg cgccttctgc
     6301 cctccacgct gcaagtgatg atcgaggagc gcgagcccta cgcggtctgg cagaacaagg
     6361 ggaagacctt tgtggtcgac gatcagggcg tcgtgctcgc gcccgctttg ccgcaggcct
     6421 atccggactt acccctcgtc gtgggcgaag gcgccagcga acatgccgcc gcgctctacg
     6481 ccacgctcca gtcttatgac cagctgaagc agaaaatgct cgcggccctc cgggtcggcg
     6541 atcgccgttg gacgctgaaa ttgcgcacgg gcacggagat catgctgccc gacgacaata
     6601 tcgaggtcgc gcttcagtcc ctcacggaac ttcagcaaga gcacggtgtt ctcgaaagag
     6661 attggtcggt catcgatttg cggctgctcg accggattac cgtgcgcacg cgcgaagcgg
     6721 ccatcgtgcc tcccgacgac attgcgccga gtgatgtgcc caccgcggca accggcacca
     6781 cgcctaaagg caaaacataa gaggcttgag ggcaggggga tgacatacag ctcgacgatc
     6841 agaagcaagc gacagcgcat cgtgaccgta ctcgacatcg gcacgagcaa agtctgctgc
     6901 ctgatcggca agacttcggc cgcgcccgac tggttcgaag gcgacggtga cgccgtgcat
     6961 ttcgaggttc tcggtttcga tcatacgcgc gccgaaggcc tgaagtccgg catggtgacg
     7021 catctcgata gcgcggagca gtgcatccgc gccgcggtcg atgccgccga gcgcatggcc
     7081 ggtgtggttg tcgaggacgt ccacgtctct gtgacggccg gccgtctgaa gagcgaaagt
     7141 ttctcggcca gcgtggcctt gcagtccggc gccgtgcgcg aggacgacgt gcatcggctt
     7201 ctggccggcg gccggcaata tgccgcccgc gacaagcgca ccgtcctgca tgccttgccg
     7261 accggctatc gtctcgatga gaacggtggc atcagcgagc cccacggcat gtgcggcgag
     7321 cgcttatccg tcgacctgca taccgtgacc gcagacgacg tggccatgcg taacctgatc
     7381 ctctgcgtcg agcggtgtca tctgggcgtc gccggtctcg tcgcagcgcc ttactcgagt
     7441 gcgctgtccg tcgttacccc ggacgaagcc aagcttggtg tggcctgcat cgacttcggc
     7501 gcgggcacga cgacgttgtc ggtcatcgcc gacggacatt tcattcacgc cgacgccatc
     7561 gcgcttggcg gcaacggcat caccacggat atcgcccgca cgctgggcgc gccgatggag
     7621 catgccgaac ggctgaagac gctgcatggt agcgctttc
//
LOCUS       DBNHVU010000148         8365 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_872115, whole genome shotgun sequence.
ACCESSION   DBNHVU010000148 DBNHVU010000000
VERSION     DBNHVU010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8365)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_872115"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="AC16Y7_05955"
     CDS             <1..460
                     /locus_tag="AC16Y7_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HIR5708959.1"
                     /translation="RRWQAEIRLITRSTALGGLAVLALVAAATIAIIVSATRSAMASN
                     REVIEVLHFVGANERFISSEFSRHFLGLGVRASLAGAFAAVIAFLLLPFLMQLLGGGA
                     VTEAETRRLVGSGVLDFGGYILLLLVVVVVAALCMVTSRFGVIRVLRTYA"
     gene            576..1286
                     /locus_tag="AC16Y7_05960"
     CDS             576..1286
                     /locus_tag="AC16Y7_05960"
                     /inference="COORDINATES: protein motif:HMM:NF014724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR5708960.1"
                     /translation="MNEDVEVQTQAPSRPQHFRRFRGFTGSMRLLFDAIILLLVMLII
                     GFIVFANGVARERQEPVHAADGITVLTGGVSRIDEAMKLLAQRKAKRVLITGVNRGTT
                     RDQLKRLASEGDQYFACCVDIDKQARNTIDNATETAQWVTLNRYGSLIVVTSNYHMPR
                     ALAELARALPGVTLAPYPVIDNNVHVDRWWKYPGTTRLLLSEYLKYLPALARLGATNL
                     ARMTLTGTSVPPEDQPEP"
     gene            1298..2023
                     /locus_tag="AC16Y7_05965"
     CDS             1298..2023
                     /locus_tag="AC16Y7_05965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR5708961.1"
                     /translation="MFRSIVFGLLFYITTVLFVVLGSPLLLGPRSWAMAALKVHAKTE
                     LWLLKHIVGLDFEVRGLDKLPEPPFLVASKHQSAWETFALIPLFRDPALLMKRELFWI
                     PLHGWFSHKFEMIPVDRDAGPSALRRMLQAARKRIKDGREIIIFPEGTRRPPGADPAY
                     KTGIVLLYNALKVPCVPVALNSGLFWPRRQWTRKPGTVVVEILDPIPPGLPRPEFLSR
                     LENAIETASDGLLAEAQSTDQAH"
     gene            2150..2563
                     /locus_tag="AC16Y7_05970"
     CDS             2150..2563
                     /locus_tag="AC16Y7_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708962.1"
                     /translation="MWTSQADAETTASTGPADEPALVCAEEAFAQAFHYWRGVSSRRY
                     LHSVYSLVGCPALPQANYILVRRHEDGTREALAFGDTKDDAISLNLAHLRHEGAKCGA
                     NEVHVHLLADTAHDRALVEADLTAAHARRHTAKAA"
     gene            complement(2593..3195)
                     /gene="phaR"
                     /locus_tag="AC16Y7_05975"
     CDS             complement(2593..3195)
                     /gene="phaR"
                     /locus_tag="AC16Y7_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HIR5708963.1"
                     /translation="MARDADQESRPVIIKKYANRRLYNTDTSTYVTLEDLAEMVRAER
                     DFVVYDAKTSEDLTHAVLTQIIVEQESRGTNLLPIGFLRQLIRFYGDSMQKLVPSYLE
                     FSLDSLTKQQEQYRRRFTNAFGNAAFDAMQDQVRKNFDAFERALGMFTQSQSDAANAH
                     GEGAEAKSEVGANPGGADDIDTLKAELEAMQQRLEKLSRG"
     gene            3387..4565
                     /locus_tag="AC16Y7_05980"
     CDS             3387..4565
                     /locus_tag="AC16Y7_05980"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR5708964.1"
                     /translation="MKDEIVIVGAARTPVGSFNGSLSSLPAHKLGEVAIGAALERAKV
                     DPSDVNEVIMGQVLTAAQGQNPARQAAIAAGISEEATAWGVNQVCGSGLRAVALGAQQ
                     IALGDADVVVAGGQENMSLSPHAAHLRNGVKMGPCNMVDTMVVDGLWEAFNNYHMGTT
                     AENVARKWQLTREEQDNFAVASQNKAEAAKKAGKFADEITPVTIKTRKGDIVVEHDEY
                     IREGATIDNIAKLRPAFDKEGTVTAGNASGINDGAAAVVLMSAEEAKKRGAKPLARIA
                     SWAVAGVDPTIMGTGPIPASRKALEKAGWKPSDLDLIEANEAFAAQALAVNKDMGWDG
                     DKVNVNGGAIAIGHPIGASGARVLVTLLHEMQKRDAKKGLATLCIGGGMGIALCVERD
                     "
     gene            4677..5399
                     /gene="phbB"
                     /locus_tag="AC16Y7_05985"
     CDS             4677..5399
                     /gene="phbB"
                     /locus_tag="AC16Y7_05985"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014766331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HIR5708965.1"
                     /translation="MGRVALVTGGTRGIGAACSKGLKDAGYSVAATYAGNDDAANAFK
                     KETGIPVFKWDVGDYQACEQGVAAVENEVGPIDVLVNNAGISRDATLHKMTEEQWNQV
                     IRTNLDSVFNVSRQVINGMRERGFGRIISISSINGQQGMMGLSNYCAAKAGMIGFTRA
                     IALEGARKGITANAIAPGYVATELLANVPEKVMDHIVGQIPVGRLGEAEEVARCVVFL
                     AADESAWITGTTLTINGGQLSD"
     gene            complement(5418..5852)
                     /locus_tag="AC16Y7_05990"
     CDS             complement(5418..5852)
                     /locus_tag="AC16Y7_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5708966.1"
                     /translation="MPSGGKTPTADEVIAHLKLQPHPEGGWFRETFRDETGPENRGHS
                     TAILFLLKAGEVSHWHRIDAVEMWHWHAGAPLLLEVADKAGRHELRLGPDIFGGEHPQ
                     GRVPAGAWQAARSLGDWTLVGCTVAPGFHFDKFELAPQGWKP"
     gene            complement(5861..6631)
                     /gene="gloB"
                     /locus_tag="AC16Y7_05995"
     CDS             complement(5861..6631)
                     /gene="gloB"
                     /locus_tag="AC16Y7_05995"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HIR5708967.1"
                     /translation="MAKLEIYQFPTRSDNYGVLIHDPDTGATAAIDAPEAAPILAALF
                     DKGWRLTDILVTHHHADHTAGIDALKKKTGCTVYGPAREAGLIPGLDVEVKEGDTVAI
                     GHADGRVIETPGHTRGHVSYHFPDENAVFVGDTLFSVGCGRLLEGNAPTMWASLSKLA
                     VLPPQTEVYCGHEYTNANCRFALTIEPDNAALQARAAQVAQNDAKGAPSLPTTIGQEL
                     ATNPFLRASSPEIQARLGMEGRPLGEIFAEVRTRKDNF"
     gene            6736..7479
                     /locus_tag="AC16Y7_06000"
     CDS             6736..7479
                     /locus_tag="AC16Y7_06000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5708968.1"
                     /translation="MHLDAIDLKDFYARRLGLLVRRLLRRRIRARWKDVKGMRVFGLG
                     FATPYLAEFRGEAHALGALMPARFGALSWPPHGPSMATLVDETELPLADETADRILLV
                     HMLEWSERSRTLLREIWRVLGPGGRLLIVVPNRRGLWARVDTTPFGYGSPFSRSQLTQ
                     LLKECMFDPEGWQYALYMPPFNWRVLMRWPTFWERLGLFLWPIFSGVILVEATKQVYA
                     AMPAREAKRKMRRRIVPVPAGVAPRKTAD"
     gene            complement(7476..8114)
                     /gene="metW"
                     /locus_tag="AC16Y7_06005"
     CDS             complement(7476..8114)
                     /gene="metW"
                     /locus_tag="AC16Y7_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HIR5708969.1"
                     /translation="MDAPVNPRNVRPTETARVDLLLIAEMVEPGSRVLDIGCGDGTLL
                     RLLAEKRDVDGRGIELSQAGVNQCVAQGLAVIQGDADTDLAYYPDLAFDYAILSQTIQ
                     ATHNPRHVLEQLLRIGKRVVVSFPNFGHWRVRTQLMFGGHMPRTDNLPETWYATPNIH
                     FCSIKDFLVLCEEIGAKVERAVALNAYGTKLVSMPLFAQNLFGEQAVFLLSR"
     gene            complement(8117..>8365)
                     /gene="metX"
                     /locus_tag="AC16Y7_06010"
     CDS             complement(8117..>8365)
                     /gene="metX"
                     /locus_tag="AC16Y7_06010"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949674.1"
                     /note="Catalyzes the conversion of acetyl-CoA and
                     L-homoserine to CoA and O-acetyl-L-homoserine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HIR5708970.1"
                     /translation="AGTKTRFCIVSFTSDWLFPTEESRDVVHALNAVAANVSFVEIES
                     NKGHDAFLLHEPELFDTVRGFLSAAARHRGVKLPDEGA"
BASE COUNT         1508 a         2717 c         2576 g         1564 t
ORIGIN      
        1 tcgccgctgg caggcggaga tccgtttgat cacgcgatcg accgcgctgg ggggcctcgc
       61 cgtgctggcc ctcgtggccg cggcgaccat tgccatcatt gtctcggcca cgcgcagcgc
      121 catggcgtcc aaccgcgaag tcatcgaggt tctgcatttc gtcggcgcca acgagcgatt
      181 catttcaagc gaattctcgc gccactttct cggacttgga gtccgcgcta gccttgccgg
      241 cgccttcgct gcggtcattg cctttttgct gctgcccttc ttaatgcagt tgctcggcgg
      301 cggcgcggta accgaggcgg agacaaggcg cctcgtcggc tccggcgtac tcgatttcgg
      361 cggctatatc cttctgctcc tcgtcgtcgt cgtcgtagct gctttgtgca tggtcacgtc
      421 gcggttcggc gtgatccgtg ttctcaggac ctacgcgtga ccccattgac ggcgccgtaa
      481 agccgatttc ttgtgcggtc aaatttgcct attctgcgcc gcgacaggac caatcgaggt
      541 aggaaagccc ctcgaaacca agctcaggac acgtggtgaa cgaagacgtc gaagtccaaa
      601 cgcaagcgcc aagtcgcccg cagcatttca ggcggttccg cggcttcacg ggcagcatgc
      661 gcctgctctt cgacgcgatc atcttgctgc tcgtcatgct catcatcggc ttcatcgtat
      721 tcgccaacgg cgtcgcccgg gagcggcagg agcccgtcca cgccgcagac ggcattacgg
      781 ttctgaccgg cggtgtctcc cgcatcgacg aggccatgaa gctgctggcg cagcgcaagg
      841 ccaagcgtgt gctcattacc ggtgtcaacc gaggcacgac gagagaccag ctgaagcggc
      901 tcgcatccga aggcgatcaa tacttcgcct gttgcgtcga tatcgacaaa caggcccgca
      961 acacgatcga caatgccacc gagaccgcgc aatgggttac gctcaaccgg tatggctcgt
     1021 tgatcgtggt cacatcgaac tatcacatgc cccgcgcgct ggccgagctc gcccgggctc
     1081 tgccgggcgt cactctcgcg ccctaccccg tgatcgacaa caatgtgcat gtcgaccgct
     1141 ggtggaagta tcccggcacc acgcggctgc ttctctcgga atatctgaaa tacctgccgg
     1201 cactcgcccg tctcggcgcc accaatctgg cgcgcatgac cttgacgggc acctcggtgc
     1261 cacctgaaga ccaaccggag ccctaaagaa ccacccgatg ttccgctcca tcgtcttcgg
     1321 tcttctcttc tacatcacca cggtgctgtt cgtggtgttg ggcagcccgc tcctgctcgg
     1381 cccaagaagc tgggccatgg cggcactgaa ggtccacgcc aagaccgagc tctggctcct
     1441 gaaacatatc gtcggcctcg atttcgaggt gcgcgggctg gataagcttc ccgagccgcc
     1501 cttcctcgtc gcctccaaac accagtcggc atgggagact ttcgcgctaa ttcccctgtt
     1561 tcgcgatccg gcgctgctca tgaaacgcga gttgttctgg atcccgcttc acggctggtt
     1621 ctcccacaag tttgagatga tccccgtgga ccgcgacgcc gggccctcgg cgctgcgtcg
     1681 catgcttcaa gccgcccgga agcgcatcaa ggacggccgg gagatcatca tcttccccga
     1741 aggaacccgc cgtccgcccg gcgcggaccc cgcgtacaaa accgggattg tcctcctcta
     1801 caacgccctg aaggtcccgt gcgtccccgt ggcgctcaat tcggggctct tctggccgcg
     1861 ccgccagtgg acgcgcaagc ccggaacggt ggtggtcgag attctcgatc ccatcccgcc
     1921 gggcctgcct cggcctgagt tcctgagccg cctcgaaaac gccatcgaaa ccgcatcaga
     1981 cggcctgctg gctgaggcac agtcgacgga tcaggcgcat taaccccgcg ttaagattta
     2041 gatgttcatt atttgttcca acaagggcga tcgaccgtgc atcttgaatt tctcgtcgat
     2101 caacccattt ctcgtagtgt cgcggatagt taacgggacg aggttttcga tgtggacgag
     2161 ccaagctgac gccgagacga ccgcctctac ggggccggcg gacgagcctg cgctggtctg
     2221 cgccgaggag gcctttgccc aggcgttcca ctattggcgt ggtgtttcga gccgccgcta
     2281 tctccatagc gtctactcgc tggtgggctg ccccgcgcta ccccaggcga actacatcct
     2341 ggtgcgccgc cacgaggacg gcacccgcga ggcgctggct ttcggcgaca ccaaggacga
     2401 cgccatctcg ctcaacctcg cccatctgcg ccacgagggc gccaagtgcg gggccaatga
     2461 ggttcacgtc catctcctcg ccgacacggc gcatgaccgt gccctggtcg aggcggacct
     2521 gaccgccgcc cacgcacgcc gccacacggc gaaagccgcg tagagctgat ttttcggaaa
     2581 atcgaagagc cgctaaccgc gagatagctt ctcgagccgc tgctgcatgg cctcaagctc
     2641 ggccttgagc gtgtcgatat cgtcagcgcc gcctggattg gcgccgacct ccgacttcgc
     2701 ctcggcgcct tccccgtgcg cattggccgc atcggattgg gactgggtga acatgccgag
     2761 cgcacgctcg aaggcatcga agttctttcg cacctgatct tgcatcgcat cgaaggccgc
     2821 attgccaaaa gcgttggtga agcggcggcg atactgttct tgctgcttgg tcaacgagtc
     2881 gaggctgaat tctagatagc tcggcacaag cttctgcatg ctgtcgccat agaagcggat
     2941 cagctgacgc aagaaaccga taggaagaag attggtgccc ctgctttcct gttccacaat
     3001 gatctgggtc aggacggcat gcgtgaggtc ttcgctggtc ttcgcgtcgt agacgacgaa
     3061 gtcccgctct gcccggacca tttccgccag gtcctcaagg gtcacatagg tgcttgtgtc
     3121 ggtattgtag agcctgcggt tggcgtattt cttgattatg accggccgac tctcttggtc
     3181 cgcgtcccta gccacgtgtt ttcgctccca aagccctgga ccttagccga gggctactct
     3241 atgccgcact tgcgaaaatc actagcacgt tccttgtgca tagcaccaga ggctttgccg
     3301 ggagcattaa tggcgacttt cgtcgggctt tccaggcgca aaccgctggg gtatgaccgg
     3361 ggctcactag gggtatggag atcaggatga aagacgagat agtcatcgtc ggcgcagcgc
     3421 ggacgccggt cggttccttc aacggctcgc tgtcgagcct gcctgcgcac aagcttggtg
     3481 aagtcgccat cggcgcagcg ctggaacgcg ccaaggtcga tccgagcgac gtcaacgaag
     3541 tgatcatggg ccaggtgctg accgcggccc agggtcagaa ccctgcccgg caggctgcca
     3601 tcgccgcagg catttccgaa gaggcgaccg cctggggcgt caatcaggtt tgcggttcgg
     3661 gcctgcgcgc cgtggcgctg ggtgcccagc agatcgcctt gggcgatgcc gacgtcgtcg
     3721 tcgccggcgg tcaggagaac atgagcctgt cgccccatgc cgcgcacttg cgcaacggcg
     3781 tgaagatggg cccctgcaac atggtcgaca ccatggtcgt ggacggcctg tgggaagcct
     3841 tcaacaacta ccacatgggc accacggcgg agaacgtcgc caggaagtgg cagctcaccc
     3901 gtgaggagca ggacaacttc gccgtcgcct cccagaacaa ggccgaggcc gcgaagaagg
     3961 ccggcaagtt cgccgacgag atcacgcctg tgacgatcaa gacccgcaag ggcgacatcg
     4021 tcgtggagca tgacgagtac attcgtgaag gcgcgacgat tgataacatc gccaagcttc
     4081 gccccgcctt cgacaaggaa ggcactgtca ccgcgggcaa cgcctcgggc atcaatgacg
     4141 gcgccgccgc cgttgttctg atgtcggccg aggaagccaa gaagcgcggc gcgaagcctc
     4201 tcgctcgcat cgcctcttgg gccgtcgcag gtgtcgatcc caccatcatg ggcaccggcc
     4261 ccatccccgc gtcccgcaag gcgctggaga aggccggctg gaagccctcc gacctcgacc
     4321 tcatcgaggc gaacgaggcc ttcgccgccc aagctcttgc cgtgaacaag gacatgggct
     4381 gggacggaga caaggtgaac gtcaacggcg gcgccatcgc catcggtcat ccgatcggtg
     4441 cgtcaggcgc gcgcgttctc gtcaccctgt tgcatgagat gcaaaagcgc gacgccaaga
     4501 agggcctcgc aacgctgtgc attggcggcg gcatgggcat cgccctgtgc gtcgagcgcg
     4561 actaacttgc ccctcttgta agggcagttg atttggtgtg gggcggccga gagccgcccc
     4621 ttacgccaat ttgtttttga ggaaacgggc gccgatgtgc gccaagggag gaagctatgg
     4681 gacgggtggc acttgtcact ggtggtacgc gcggcattgg agccgcctgc agcaagggac
     4741 tgaaggatgc gggctacagc gttgccgcga cttatgccgg caacgatgat gccgcgaacg
     4801 ctttcaagaa ggagaccggc atcccggtct tcaagtggga cgtcggcgat taccaggcct
     4861 gcgaacaggg tgtcgcggct gtcgagaacg aagtcggccc catcgatgtg ctggtcaaca
     4921 atgccggcat ctcgcgcgac gccacgctcc acaagatgac cgaagagcag tggaaccagg
     4981 tcatccgcac caatctcgat tcggtgttca acgtgagccg ccaagtcatc aacggcatgc
     5041 gcgagcgtgg tttcgggcgc atcatctcga tctcgtcgat caacggccag cagggcatga
     5101 tgggcttgtc caattactgc gccgccaagg ccggcatgat cggcttcacc cgcgccatcg
     5161 cgcttgaagg cgcacgcaag ggcatcactg ccaacgccat cgcccccggc tacgtggcga
     5221 ccgagctcct ggccaacgtg ccggagaagg tcatggatca catcgtcggc cagatcccgg
     5281 taggccgcct cggcgaagcg gaagaagtcg cccgctgcgt ggtgttcctc gccgccgatg
     5341 aatccgcttg gatcaccggc acgacgctga ccatcaacgg cggtcagctc tccgactaaa
     5401 acacggacgt tcgtccctca gggcttccag ccttgagggg cgagctcgaa cttgtcgaaa
     5461 tggaagccgg gcgccaccgt gcagcccacg agcgtccagt caccgagact gcgcgcagct
     5521 tgccacgccc ccgccggcac acggccttgc ggatgctccc cgccgaaaat atccggccct
     5581 agccgaagtt cgtgacggcc ggccttgtcg gcgacctcca ggaggagcgg cgcgccggcg
     5641 tgccagtgcc acatctcgac cgcatcgatg cggtgccagt gggacacctc gccggccttg
     5701 agcaaaaaga ggatcgcggt ggaatgcccg cggttctcgg gaccggtttc gtcccggaag
     5761 gtctcgcgga accagccgcc ttcgggatgg ggttgaagct tcagatgcgc gatcacttcg
     5821 tcagcggtcg gcgtcttacc cccgctcggc atcttgccca ctagaaattg tccttgcgcg
     5881 tccgcacctc ggcgaaaatc tctccgagcg ggcgcccttc catgccgagc cgcgcttgga
     5941 tctccgggct cgaggctcgc aggaacggat tcgttgcaag ctcttgaccg atcgtcgtcg
     6001 gcagcgacgg cgcgcctttc gcatcgttct gcgcaacttg cgcggcgcgg gcttgtagcg
     6061 ccgcattgtc gggctcgatg gtgagcgcga agcggcaatt ggcgttcgtg tattcgtgac
     6121 cgcaatagac ctccgtctgc ggcggcagca cggccagctt cgataacgaa gcccacatgg
     6181 tcggcgcatt gccctccagc agcctgccac accccaccga aaacagcgtg tcgcccacga
     6241 acaccgcatt ctcgtccggg aagtggtagc tcacatgccc gcgcgtatgg cccggcgtct
     6301 cgatgacgcg gccatcggca tgacctatgg caaccgtgtc gccttccttc acctccacgt
     6361 cgagcccggg gataagtccg gcctcgcgcg ccggtccata gaccgtgcag ccggtctttt
     6421 tcttcagagc gtcgatgccc gccgtatgat cggcatggtg atgggtgacg agaatgtcgg
     6481 tcagccgcca tcccttgtcg aacagcgccg ccaggatggg cgccgcctcg ggcgcgtcga
     6541 tcgccgccgt ggccccggtg tcgggatcgt ggatcaaaac cccgtaattg tcgctccgcg
     6601 tcggaaattg ataaatctca agcttggcca tcacgcactc ttctttcttg cttatttgtc
     6661 gaagcgacgg gaccctagca ccagcaacgc gcaaattgga acgaactcta aaggcgaaag
     6721 tccgacactt cggccatgca tcttgatgcc atcgacctca aggatttcta cgcaaggcgc
     6781 ttgggcctct tggtgcggcg gctgctgcgg cgtcgcatcc gcgcgcgctg gaaggacgtc
     6841 aagggcatgc gggtgttcgg ccttggcttc gccacgccct atttggccga gttccgcggc
     6901 gaggcccatg cgttgggcgc gctgatgccg gccaggttcg gcgccctgtc ctggccaccc
     6961 cacggaccgt ccatggccac gctggtggac gagacggaac tgccgctggc cgacgaaacg
     7021 gccgaccgca tccttctggt gcatatgctg gaatggtccg agcgatcacg cacattactg
     7081 cgcgagatct ggcgcgtgct cgggcccggc gggcggctct tgatcgtcgt ccccaaccgg
     7141 cggggtctat gggcacgcgt ggacacgaca cccttcggct atggcagccc gtttagccgc
     7201 tcccagctca cccagcttct caaagagtgc atgttcgatc ccgaaggctg gcaatacgcg
     7261 ctctacatgc cgccgttcaa ttggcgcgtg ctgatgcgct ggccgacctt ctgggaacgg
     7321 cttggcctgt tcctgtggcc gatcttctcc ggcgtgatct tggtggaggc gacgaagcag
     7381 gtctatgcgg cgatgccggc gcgcgaggcc aaacgcaaga tgcgccgccg catcgtgccg
     7441 gtgcctgccg gcgtggcgcc gcgcaagacg gccgactagc gcgacagcaa aaagaccgcc
     7501 tgctcgccga acaagttctg ggcgaatagc ggcatagaga cgagcttagt gccgtaggca
     7561 ttgagtgcca cggcgcgctc gactttggcg ccgatctcct cgcacagcac cagaaaatcc
     7621 ttgatggagc agaagtggat attcggcgtg gcgtaccagg tctccggcaa attgtcggtg
     7681 cgcggcatgt ggccgccgaa catcagctgc gtgcgcacgc gccaatgccc gaaattggga
     7741 aacgacacca cgacgcgctt gccgatgcgt aagagctgct cgagcacatg gcgcgggttg
     7801 tgcgtcgcct ggatggtctg cgacaggatc gcgtagtcga aagccagatc cgggtagtag
     7861 gcgagatccg tgtcggcgtc gccttggatc accgccagcc cttgcgccac gcattggttc
     7921 acgccggcct gactcagctc gatgccgcgc ccgtcgacgt cacgcttctc ggcgagaagc
     7981 cgcaacagcg tgccgtcgcc gcagccgata tcgagcacgc gcgagcccgg ctccaccatc
     8041 tcggcaatga gcagcaggtc gacgcgggcc gtttcggtag gccgcacgtt gcggggattg
     8101 accggtgcgt ccatggtcac gcgccttcgt ccggaagttt gacgccgcga tggcgcgccg
     8161 ccgcgctcaa gaaaccgcgc actgtgtcga acagctcggg ctcgtgcagt aagaaggcgt
     8221 cgtgtccttt gttggactcg atctccacga aactcacatt ggcggcgacc gcgttcaacg
     8281 catgcaccac atcgcggctc tcctcggttg ggaacagcca atcggaggtg aacgacacga
     8341 tgcaaaaacg cgtcttcgtg ccggc
//
LOCUS       DBNHVU010000149         7874 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_2060527, whole genome shotgun sequence.
ACCESSION   DBNHVU010000149 DBNHVU010000000
VERSION     DBNHVU010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7874)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7874)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7874
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_2060527"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..1260
                     /locus_tag="AC16Y7_06015"
     CDS             343..1260
                     /locus_tag="AC16Y7_06015"
                     /inference="COORDINATES: protein motif:HMM:NF013355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR5708971.1"
                     /translation="MNLSTRYLGLELKHPIVASPSPLTRDMDGIRKLLDAGAAAIVVA
                     SVFEEQVNADRGVLDERLETVRQASKQGSVPIMGSLNGETLGGWVSFATQLEEAGASA
                     IELDLYRVPSDPNETGQALEESYVDILRAVKSKVKVPVAVKLSPYFSAPANMAKQLVD
                     AGADGLVLFNRFYEGDIDLTTQLVKPDFRLSTPYDSRLPLMWISLLSGRLNASLAATS
                     GVWTYEEVVKDLLVGADAVMTTSSLLKDGPQHLETLVRGLREWMEARGFDSLADFKGQ
                     LAAAHLYDPSAFMRVHYSDILTSSGKDYA"
     gene            1498..2739
                     /locus_tag="AC16Y7_06020"
     CDS             1498..2739
                     /locus_tag="AC16Y7_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HIR5708972.1"
                     /translation="MRTTIISILCGAVIGTALSGPAIAGEQLVMPFACRVAGGQILLA
                     PSEPRTYQIYGQRAHKRLTTCSPYDPRRCHTWSVHRFDLDCGGVRTSWPAVVAALSPI
                     LADSIGYGRPGGPHPAPPRPHRGPGASAFPGGFAPNPMRVARFEHVNLAPAVDLAPVN
                     VPIPLKKPAPAEPTVEVAQTAPNVEPVAEPPSLPDEVETPPASAPAEDNDADKATALA
                     QQRALQVEAGPADSDVTGALPKPKTSSLFRDAGLVFVFTVAALLALSAALLLRQRGLA
                     LLPARIPLLLRGPNTLPALRRDAVAEHESPQAHEQHETIEAPAVHLRLWDKDWLPKTK
                     AEALNVLGVDPAASADKIKSTVTRLRRALHPDHAFDDEDRDLRERRLKQINVAWEIVS
                     EKRRAPWLAVKPQSSWPLWRT"
     gene            complement(2901..4790)
                     /gene="thiC"
                     /locus_tag="AC16Y7_06025"
     CDS             complement(2901..4790)
                     /gene="thiC"
                     /locus_tag="AC16Y7_06025"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR5708973.1"
                     /translation="MNVHDKSLRGETTLNVTTGPLTGSCKVYSSPGGHEAVRVPLREI
                     ELTNGDTFNVYDSSGPYTDSDAAIDVKRGLDPLRSSWIEARGGVETYEGRAIKPEDNG
                     NVGAAHLAESFQATRAPLHGLEGRPVTQLELARAGIVTDEMVYIAHRENIGRQQAADE
                     AQERRDDGEDFGASIPAFVTPDFVRDEVARGRAIIPANINHPESEPMIIGRNFLTKIN
                     ANIGNSAVTSSVEEEVEKMVWAIRWGADTVMDLSTGRNIHTTREWIIRNAPVPIGTVP
                     IYQALEKVDGDPVKLDWEVYKDTLIEQCEQGVDYFTIHAGVRLGYIHLTADRVTGIVS
                     RGGSIMAKWCLAHHRESFLYERFADICEIMRKYDVSFSLGDGLRPGSIADANDPAQFA
                     ELETLGELTRVAWDRGCQVMIEGPGHVPMHKVKVNVEKQLKECGEAPFYTLGPLVTDI
                     APAYDHITSAIGAGMIGWFGTAMLCYVTPKEHLGLPDRDDVKQGVIAYKIAAHAADLA
                     KGHPAAQARDDAISEARFGFRWEDQFNLSLDPDTAREYHDETMPKEAHKLAHFCSMCG
                     PKFCSMEITREVRDYAAKLNDKNMLSSSEAVEQAKKQGMAEMSAKFRELGSEVYVEED
                     ESPAR"
     gene            complement(4801..5196)
                     /locus_tag="AC16Y7_06030"
     CDS             complement(4801..5196)
                     /locus_tag="AC16Y7_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708974.1"
                     /translation="MQTSIFLAQLIGPVMLAAAVSMIINREGQRAMAKDFLDSPPLIY
                     ISGVLVMLAGLAMVLYHNVWVADWRVIVTLLGWMATIGGAIRILCPSAVKKMGAAMID
                     KPMAMTIGSLVWLVIAVVLLYFGYFGYFA"
     regulatory      complement(5192..5318)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5446..5814)
                     /locus_tag="AC16Y7_06035"
     CDS             complement(5446..5814)
                     /locus_tag="AC16Y7_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708975.1"
                     /translation="MKSNWRAFALAVIAAGFCLGQSTTAEAGCVGLNGTADGVNKTTA
                     VSRSQNALATAVQEFKASKRIRNVRISPMRPKPQPYWRSSVSPDLYQKPDVVTSKTHT
                     VCWSGVVSPTVCSTGARVCW"
     gene            5986..6786
                     /locus_tag="AC16Y7_06040"
     CDS             5986..6786
                     /locus_tag="AC16Y7_06040"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5708976.1"
                     /translation="MTAKLVLVTGASSGIGEATAKRYGAAGAHVLLIARNAERLYGVA
                     HAIREAGGTATAFPLDLAGSEATKEVAARIEAEIGTPDVLINNAGAGRWLPLIETKAE
                     DARAMIDVPYLAAFNLTRAFAPAMIARGTGSLVFVTSPAAYLAWPNASAYIAARRAVA
                     GLAEALQSELKPTGVSVTLVVLGSVETPYWQHNPGSLQNVPEDPPFVRALTADEAAKA
                     IVQGAARKKKFVIKPGIYRALFVLGALFPKTVASQLRRAAKRGRATKP"
     gene            complement(6814..7287)
                     /locus_tag="AC16Y7_06045"
     CDS             complement(6814..7287)
                     /locus_tag="AC16Y7_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708977.1"
                     /translation="MRIGLAVLATFCLSVFVCAPAVAACYGTNAKTKQRQAVHEIYRG
                     SSHPKRYFAQATHDASTGRPQIFYCRRYSSAPAHFKRFVRAHECCHHLGYHNEIAANC
                     CALRRMRLSSSGLAALRNYIVSRDVNSQTKVDQKGQGSTFWNKTARQCNLGVRID"
     gene            complement(7357..>7874)
                     /locus_tag="AC16Y7_06050"
     CDS             complement(7357..>7874)
                     /locus_tag="AC16Y7_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIR5708978.1"
                     /translation="VGSSEGLYAILGMGAVAAAVLGAPISTTVMVFELTGGFELSLAL
                     LLTVAIANGINQAILGRSFFQAQLESRGIVLHEGPHRTVMKDIRVADFMWPLKDGDPT
                     VLPEGERERALQSNETLETALRAFDTMGAERLPVVEPGDRSRIVGFATQARALRVFNR
                     GLIAASVEEHR"
BASE COUNT         1430 a         2604 c         2405 g         1435 t
ORIGIN      
        1 gtcttgcacc ttctcagggt cgagcgcatc ggaatggtgc ggcgcgaagc gtccttcatc
       61 attgtcaaaa tactgacccg atgcgtctgc gaagtcgtcc gccaatgccg cccgggtcag
      121 gatctccgcg ccgatgccgt cggtggcgcc tgtgatgaga atggtctttg gcatgattgt
      181 gtccgcctac tccccctcag agtccaaagg cccgtcgtcg tctgccgaac tagatctgga
      241 tcaaggccaa ggcgttggcg tttgtctgat agtgttgccc agtcgatgga ccatcctcct
      301 gaccagagga tggcggatcc cctggtaccg tggaggcccc tcatgaatct gagcacccgc
      361 tatctcggac tcgagctcaa gcacccgatc gtcgcctcgc cgtcgccgct gacccgcgac
      421 atggacggga tacgcaagct cttggacgca ggcgccgcgg ccatcgttgt cgcgtccgta
      481 ttcgaggagc aggtcaacgc cgaccgcggc gtgctggacg aacgtttgga gactgtgcgc
      541 caggcgtcga agcaaggcag cgtcccgatc atgggcagcc tgaacggcga gaccctgggc
      601 gggtgggtga gctttgccac acagctcgag gaggccggcg cgagcgcgat cgagctcgac
      661 ctttatcgcg tcccgtccga tcccaacgag acaggccagg cgctcgaaga gagctatgtc
      721 gacattctcc gtgccgtcaa atccaaggtg aaggtgccgg tggccgtcaa gctcagcccc
      781 tatttcagcg cgccggcaaa catggcaaag cagctcgtgg acgccggcgc cgatggccta
      841 gtcttgttca accggttcta cgaaggcgac atcgatttga cgacgcagct cgtgaagccg
      901 gacttccggc tcagcacgcc ctatgacagc cgcctgccat tgatgtggat atcgcttctg
      961 tcggggaggc tgaatgcgtc gctcgccgcg acctccggcg tctggaccta tgaagaggtc
     1021 gtgaaagacc tcctggtcgg agccgatgcg gtcatgacga cgtcgtcgct gctgaaggac
     1081 ggcccgcagc accttgaaac gctggtgcgc ggcttgcgcg agtggatgga agcccgcggg
     1141 ttcgactctc ttgccgactt caagggccag ctcgcagccg cgcatcttta cgacccgtcg
     1201 gcgttcatgc gcgtccatta cagcgacatc ctgacgtcca gcggcaagga ttatgcgtaa
     1261 agaagcgtgc aggtcacggc gtgctcattc tcattgggcc gatgaggaca tcgtggcctc
     1321 cttcgagggg cctgcagcgg cagccaatcc agtctttagg tcgaggaaac gttttggctt
     1381 cagcatgaag gccggaatct gaggatacga cggggcgttt cggcctgttt tcatcacatc
     1441 gtcgccaaga tttggcgaaa agtcggggct acgatccctg cccatcggga ctttacagtg
     1501 cgcacaacaa tcataagcat tctgtgcggc gccgttatcg gcaccgcgct cagcggcccg
     1561 gcaatagccg gggagcagct ggtgatgccc ttcgcctgcc gcgtcgcagg cggacagatt
     1621 ctgctcgctc ccagtgagcc gcgcacttac cagatctacg gccaacgggc ccacaagcgg
     1681 ctaaccacct gctcccctta cgatccgcgg cggtgccaca catggtccgt ccatcgcttc
     1741 gatctcgact gcggcggcgt ccggacgagc tggccagccg tggtggccgc gctgtcgcca
     1801 atcctggcag acagcatagg atatggccgt ccgggcggac cgcacccagc gcccccgcgg
     1861 ccccaccgcg ggcccggcgc cagcgccttt cccggcggct tcgcgcccaa ccccatgcgg
     1921 gtcgcccgtt tcgagcacgt taacctggcg cccgcggttg acctggcacc cgtgaacgtc
     1981 ccgattccgc tgaagaagcc agccccggcg gagcccaccg ttgaggttgc ccagaccgcg
     2041 ccaaacgtcg agccagtggc cgagcctccc tcgctccctg acgaagtgga gacgcccccc
     2101 gcgagcgccc cggccgagga caatgatgca gacaaggcaa cggcccttgc gcagcagcgc
     2161 gcattgcaag tcgaagcagg ccccgccgac agcgacgtga ccggcgccct gccaaaaccc
     2221 aagacaagct cgctatttcg ggacgcaggc ctcgtcttcg tcttcacggt cgccgccctg
     2281 ctcgctttgt ccgccgcgct ccttttgcga cagcgcggat tggcgcttct gccggcgcgg
     2341 atcccgttgc tgttacgcgg gcccaacaca ctgcctgccc tgcggcgcga cgcggtcgcg
     2401 gagcacgaga gcccgcaggc gcacgagcag cacgagacaa tcgaagcccc cgccgtccac
     2461 ttacggctat gggacaagga ttggctgccg aagacgaagg ccgaagccct caatgtcctc
     2521 ggcgtcgacc cggccgcgag cgccgacaaa atcaagagca ccgtcacgcg gctacgccgc
     2581 gcgctccacc ccgatcacgc tttcgacgac gaagaccgcg acttgcgcga acgccgcctg
     2641 aagcagatca acgtggcctg ggaaatcgtc tcggagaaac ggcgcgcgcc gtggcttgcg
     2701 gtcaagccgc aatcctcctg gcccctgtgg cgcacatagg cttactcggg cactcaacca
     2761 ggagcgctca aatcgcgcga agcgcgatag actcacccgc cccccgcccc gaacgacaga
     2821 cggtgatggt tgtcctattc tcgtggttga ctcggcatgt agcaaagcca gtgttcctgg
     2881 cgttccattg cggccccgac tcagcgcgcg ggcgactcgt cttcttccac gtagacctcg
     2941 ctgcccagct cgcggaattt ggcgctcatc tccgccatgc cctgcttctt tgcctgctct
     3001 accgcttcgc tacttgagag catgttcttg tcgttgagct tggcggcgta gtcgcgcact
     3061 tcgcgcgtga tctccatgga gcagaatttc ggtccgcaca tggagcaaaa gtgcgcaagc
     3121 ttgtgcgcct ccttcggcat ggtctcgtcg tgatattccc tcgccgtgtc gggatcgagc
     3181 gacaggttga actgatcctc ccagcggaag ccgaagcgtg cttctgagat ggcgtcgtcg
     3241 cgggcttgcg cggccgggtg ccccttggcg aggtcggcgg cgtgcgccgc gatcttgtag
     3301 gcgatgacgc cttgcttcac gtcgtcacga tccgggaggc caaggtgctc cttcggcgtg
     3361 acgtagcaaa gcatcgccgt gccgaaccag ccgatcatgc cggcgccaat cgccgacgtg
     3421 atgtggtcgt aggccggcgc gatgtcggtg accaaaggcc cgagcgtata gaacggcgcc
     3481 tcgccgcatt ccttcagctg cttctccaca ttgaccttga ccttgtgcat cgggacatgg
     3541 cccggccctt cgatcatcac ctggcagccg cgatcccaag cgacgcgtgt gagttcgccc
     3601 agcgtctcca actcggcgaa ctgtgcgggg tcattggcat ctgcaatgga gcccggccgc
     3661 aagccatcgc cgaggctgaa cgacacgtcg tatttccgca tgatctcgca aatgtcggcg
     3721 aagcgctcgt agaggaagct ctctctgtga tgcgccaggc accacttggc catgatcgag
     3781 ccgccgcgtg acacgatgcc ggtgacgcga tccgccgtga gatggatata gccgaggcgc
     3841 acgcccgcat ggatcgtgaa gtagtcgacg ccctgctcgc attgctcgat cagcgtgtcc
     3901 ttgtagacct cccagtcgag cttgacggga tcgccgtcca ccttctccag cgcctgatag
     3961 attggcaccg tgccaatggg caccggcgca ttgcggatga tccattctcg ggtcgtatgg
     4021 atgttgcgtc ccgtagagag atccatgacc gtgtcggcgc cccagcggat cgcccacacc
     4081 atcttctcca cttcttcttc gaccgaagac gtcacggcgg agttgccgat attggcgttg
     4141 atcttggtca gaaagttgcg gccgatgatc atcggctcgg actcggggtg attgatgttg
     4201 gccgggatga tggcccggcc gcgcgccacc tcatcgcgca cgaagtctgg cgtgacgaac
     4261 gcggggatag aggcgccgaa atcctcgccg tcgtcgcggc gttcttgcgc ttcgtctgcc
     4321 gcctgttggc gcccgatgtt ttcgcgatgc gcgatataga ccatctcgtc cgtgacgatc
     4381 cctgcgcgcg cgagctcgag ttgcgtaaca ggcctgcctt cgagcccgtg caagggtgcg
     4441 cgcgtggctt gaaagctttc cgccaaatgc gccgcgccga cgttcccatt gtcttcgggc
     4501 ttgatcgcgc ggccttcata cgtctcgaca ccgccgcggg cttcgatcca tgagctacgc
     4561 aacggatcga gaccgcgctt cacgtcgatc gcggcgtcgc tgtcggtgta cgggcccgac
     4621 gagtcgtaga cattaaaggt gtcgccattc gtgagttcga tctcacgcaa cggcacgcgc
     4681 accgcctcat gcccgcccgg cgacgaatag actttgcagc tgccggtcag cgggcctgtg
     4741 gtgacgttga gcgttgtctc gccgcgaagc gacttgtcat gcacattcat gtcattggtc
     4801 tcacgcaaaa tagccgaaat agccgaaata gagaagcact acggcaatga cgagccagac
     4861 caagctgccg atggtcatgg ccatgggctt gtcgatcatc gccgcgccca tcttcttgac
     4921 cgcgctgggg caaaggatgc ggatcgcgcc gccgatcgtc gccatccaac ccaacagcgt
     4981 gacgatcacg cgccaatcgg caacccacac attgtgatag agcaccatcg caaggccggc
     5041 caacatgacg agcacgccgg agatgtagat caacggcggg ctgtccagaa agtcctttgc
     5101 catggcccgc tggccttcgc ggttgatgat catgctgacg gcggcggcca gcatgaccgg
     5161 gccgatgagt tgcgcaagaa agatggatgt ctgcatggct gtgtcctgtc ccttcgccgg
     5221 tatgagccgg atcaggttct aagggtcttc gctccggccc agtgaaaaca cgagcaacga
     5281 actctcagcc cctccccggg gctcccctcg gaacgggatc actgtagcgc cttgcgccgt
     5341 gctccgaaag ggcaagcacg cgccaatccg ctgcaacgcg cgcacgccac agcccggaga
     5401 catgcgtccc cggggatcaa gccatggata aaacagcggc tgcggctacc agcagaccct
     5461 ggcgcccgtc gaacacaccg tcggcgacac cacacccgac cagcagacag tgtgcgtctt
     5521 cgacgtcacc acatccggtt tctggtagag atccggcgaa acgctgctgc gccaatacgg
     5581 ctgaggcttc ggccgcatgg ggctgattct cacgttgcga atgcgcttcg aagccttgaa
     5641 ctcctgaacc gccgtcgcga gcgcattctg ggaacggctg accgccgttg tcttattcac
     5701 cccgtccgcg gtcccgttca acccaacgca gccagcttcc gccgtggtgc tctggcctaa
     5761 gcaaaatcca gccgcgatca ctgccagcgc aaaggcacgc caattcgact tcatcgaagc
     5821 ctccctaaac caagctaaga ttggctggat tatcgcgggg cgtgggcgcg gtttccagcg
     5881 tcttacacca tcctgcaacg cgttcggcga attctgttac ctttgtgcca cagtgcgagg
     5941 cgatttcgac gccgcttgcg gccgcgcacg cgggagggag gggaaatgac agcgaaactg
     6001 gtgctcgtca ctggcgcctc cagcggcatc ggcgaggcca cggcaaagcg ctatggggcc
     6061 gcaggcgccc atgtcctcct gatcgcccgc aatgcggagc gcctttatgg ggtagcccat
     6121 gcgatccgtg aggcgggcgg tacggcaacg gccttcccct tggacctggc cggctcggag
     6181 gccacgaaag aggtcgccgc ccgcatcgag gccgagatcg gcacgcccga cgttctcatc
     6241 aataacgccg gcgccggccg ctggctgccg ctcatcgaaa ctaaagccga agatgcccgc
     6301 gccatgatcg acgtgccgta tctggcggcc ttcaacctca cacgggcctt cgcaccggcc
     6361 atgatcgcgc gcggcacggg ctccctcgtc ttcgtcacct cgccggccgc ctatctcgcc
     6421 tggcctaacg cctctgccta tatcgccgcc cgccgggccg tggccggcct cgccgaagcc
     6481 ctgcaaagcg aactgaagcc gacgggcgtc tccgtgaccc tggtcgtgct gggctccgtc
     6541 gagacgccct attggcagca caaccctggg agcctccaga atgtgcccga ggacccgccc
     6601 ttcgtccggg ccctgaccgc ggacgaagcc gccaaggcca tcgtccaagg cgccgcgcgg
     6661 aaaaagaaat tcgtcatcaa gccgggcatc taccgggcgc tgtttgtact gggcgcgctc
     6721 tttccgaaga ccgtggcgag ccaacttcgt cgtgccgcca agaggggacg cgccaccaag
     6781 ccctagcggc ggaacaggcc gagccgatga agctcagtcg atgcggacgc caagattgca
     6841 ctgccgcgcg gtcttgttcc agaaggtgct gccctgcccc ttttggtcga ccttggtttg
     6901 gctgttgacg tcgcgggaaa cgatgtagtt ccggagcgca gccaagccgc tggacgacaa
     6961 gcgcatgcgc cgtaatgcac aacaattggc ggcgatctcg ttgtggtaac cgagatggtg
     7021 acagcactcg tgggcccgga cgaagcgctt gaagtgggcg ggcgcgctgc tgtagcggcg
     7081 acagtagaag atctgcggcc gaccagtgct cgcatcgtgc gtcgcttgcg cgaagtatcg
     7141 cttcggatga ctgctgccgc gatagatttc atgcacggct tggcgctgct tggtcttggc
     7201 gttggtgccg tagcaggccg ccacggccgg tgcacatacg aacaccgaca aacagaaggt
     7261 cgccaaaacg gccaggccaa tgcgcatgga tcgctactcc ctggcgaaac ataatccgcc
     7321 acgcagggcc ggaccacagt ggcagatcaa tattacttaa cgatgttctt ccacgctcgc
     7381 cgcgatcaac cctctattga agacgcgcag tgcacgggcc tgcgtggcga agccgacaat
     7441 ccggctccgg tcgccgggct ccacgacggg caatcgctcg gcgcccatcg tatcgaacgc
     7501 ccgcaaggcc gtttccaacg tctcgttgga ttgcagcgcc cgttcgcgtt ctccctccgg
     7561 caggacggtg ggatcgccgt ctttcagcgg ccacatgaag tcagcgacgc gaatatcctt
     7621 catcaccgtg cgatgcggcc cctcgtgcag gacgatgccg cgcgattcca gttgcgcctg
     7681 gaagaacgaa cggccgagga tcgcctggtt gatgccgttc gcgatggcca cggtaagcaa
     7741 cagagcgagg ctcaactcga agccgccggt caactcgaac accatcaccg tggtggagat
     7801 tggcgcgccg agcacggcgg ccgccaccgc gcccatgccg agaatggcgt agaggccttc
     7861 gctcgagccc actt
//
LOCUS       DBNHVU010000150         6968 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1912221, whole genome shotgun sequence.
ACCESSION   DBNHVU010000150 DBNHVU010000000
VERSION     DBNHVU010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6968)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6968)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6968
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1912221"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..416)
                     /locus_tag="AC16Y7_06055"
     CDS             complement(96..416)
                     /locus_tag="AC16Y7_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708979.1"
                     /translation="MSHDFSPVSEGYHGIYHLAHDVAARRGHTRPAGSAVRSEDQVVS
                     EEAKFREVKLDRATADLLEALARARGLTLAQLLAEYAAADSSMPGRADIKTGTLLALG
                     VANR"
     gene            complement(764..1405)
                     /gene="chpT"
                     /locus_tag="AC16Y7_06060"
     CDS             complement(764..1405)
                     /gene="chpT"
                     /locus_tag="AC16Y7_06060"
                     /EC_number="2.7.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0018197 - peptidyl-aspartic acid
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase ChpT"
                     /protein_id="HIR5708980.1"
                     /translation="MTEPTGLTDLELAALVSSRICHDVINPVAAISNGLEMLGEEQDA
                     DMREAAMDLIRKSATQASAKLQFARLAFGAAGSAGAEIDLRDAEKVSREFVAGTGKHE
                     VIWEGPVVTLPKNQVKALLNLVALGVVALPRGGTVSVEIAGNPPEAVSFTVRAKGKAA
                     RLNEQVTALLSGSNGVEIDAHSIQPYYASRVATAAGLSLTAQAEDDEVTLKGA"
     gene            1548..2408
                     /gene="cysQ"
                     /locus_tag="AC16Y7_06065"
     CDS             1548..2408
                     /gene="cysQ"
                     /locus_tag="AC16Y7_06065"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008935242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HIR5708981.1"
                     /translation="MSAPRRTTALTSQAGTDFDLGQAAAVLTDAAAKAGAAIMAHYAG
                     PVEVETKDDESPVTKADRDAEAAILPVLAALAPSISVVSEETAADRTEALGQRFFLVD
                     PLDGTKEFIKKRSDFTVNVALIEEGRPRFGLVYAPARSLLAVTTADGVVVEAEMAPSA
                     AGADLDALPQKRLHARAGDPKALTALVSLSHLDPKTEAFLEGLDIAERSSAGSSVKFL
                     EIARGNADVYPRFGPTMEWDTAAGQAVLEASGGRVVDMEGNRFGYGKTEDGLRNSGFV
                     AWGRTILTPQ"
     gene            2513..3268
                     /locus_tag="AC16Y7_06070"
     CDS             2513..3268
                     /locus_tag="AC16Y7_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1134 domain-containing protein"
                     /protein_id="HIR5708982.1"
                     /translation="MRISTLTIAASSAILLTCFSLTASAQDDQMVITPESYADMIDGR
                     EPSQLPPPRSSGAESAGTPATYPQGGVTETYQPSNGYASETLPGSDDDTFSVEEISGA
                     GHRFFGKVSVGFAKAVEYAFQKSGRPNGYILGEEAGGAFVAGLRYGEGILYTKDAGQH
                     RVFWQGPSVGYDFGAAGSKTMVLVYNLSNPAQIYDRFAGVDGSAYFIGGVGVTYLEGD
                     KLVLAPIRSGVGLRLGANIGYLKYTRQPTWNPF"
     gene            3396..4703
                     /locus_tag="AC16Y7_06075"
     CDS             3396..4703
                     /locus_tag="AC16Y7_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708983.1"
                     /translation="MIEWFMFGALGFFLGCLLALMLAPPLWNRAVKLTTRRLEATMPL
                     TLADIQADKDQLRAEFAIELRKVEVALDKSKEKAARELIEANKRRVEIQELNTEIAGV
                     KGKLTESQNANRVLQQTIKRRLPDLDTRLKAAKKALADLENVNAELRNTVASQSEALK
                     IARSTVQNQRADIDRLRLSLESGSAGSMRGSSKSDGRAVAESQRLAAELSRATEELER
                     AKTGAEEIAFLRRELNRLASHIMTAARNQAQHAQPAAAAQEHAEYAQSDTTSEVTTED
                     AAWHAEAGNGEAAEPMAEEAPEEIVAAEGEHMEYAEAEAEVEVEAEVEVEAEAEAEIE
                     HEVKAEAEVESVEQARFEDAVEFEEADPETHAEIEPDEYEDVPHEGHDEEVYAEEDAD
                     EDEDEQRTSLAQRFAARRSKREKGGSLTSRLRGVVAKAPETRA"
     gene            complement(4707..5414)
                     /locus_tag="AC16Y7_06080"
     CDS             complement(4707..5414)
                     /locus_tag="AC16Y7_06080"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR5708984.1"
                     /translation="MPKGELIYAVGDIHGRSDLLAELLLKIEADAAMRLAAKKRLIFL
                     GDYVDRGPDSRGVVEILLHGLPSDFSAHFLKGNHEALLLGFLADASRLDQWRYNGGEE
                     TMASYGVDVGWLKEVRAAPEAWREAFASALPATHLHFLQGLDLLIPADDYLFVHAGVR
                     PGVPLEAQSETDLIWIRDEFLGSTKSFGKVVVHGHTPALEPVMRPNRIGIDTGAVFSG
                     RLTALRLQDGERSFLET"
     gene            complement(5433..5870)
                     /locus_tag="AC16Y7_06085"
     CDS             complement(5433..5870)
                     /locus_tag="AC16Y7_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708985.1"
                     /translation="MPLWSKIATGLAIAVTLLGFVGFRAYSERLGPIVQTYGTTYYNG
                     AGPQAPAVPCKSKSEIRSAIARVHAKFDHLEGESLKAFEERAALLKGLPPLRVEELYV
                     ITEDDKLRDGKMVLFIGLKSNCVSTVFSFPAPLYRELVASGAA"
     gene            complement(5937..6335)
                     /locus_tag="AC16Y7_06090"
     CDS             complement(5937..6335)
                     /locus_tag="AC16Y7_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708986.1"
                     /translation="MPVPMPAAVVLIVCKTVSAGPEDHNAKYTGYENLEWATEHSMMV
                     CRRQEVQLYDRAEALGAARQPFNLQRCQRSGIMLGTQWDASHRGSKYRFWRVACPVPI
                     VDTRTGDVIAWKMPECGHRDTVRCEVDAEI"
BASE COUNT         1393 a         2226 c         2109 g         1240 t
ORIGIN      
        1 gcacggacga agccgccgcc taacctcact cagcaaattt tgagatacgt caatggccgg
       61 gctagtcccg gccattgtca ttttggggat ccgattcagc gattggcaac gccgagggct
      121 agcagtgtcc cggtcttgat atcggcccgc cccggcatag aggagtccgc ggccgcgtat
      181 tccgccaaga gctgtgccag cgtcagaccg cgggccctcg ccaacgcctc gagcagatcg
      241 gccgtcgcgc gatccagctt gacctcgcgg aacttagcct cctccgaaac cacctgatcc
      301 tcgctcctga cggcggaacc agccggccgt gtgtggcccc ggcgggccgc gacgtcatgc
      361 gccagatggt agataccgtg gtagccctct gagacgggcg agaagtcgtg cgacacgccc
      421 ctgacggtct ccgcggagcc aaggacgaga tagccatccg tcgaaagctg acgcgccaga
      481 cgcgacagga cagtgcggcg caggtccgga agatggcgtt gagcatctcg gcatcgttgg
      541 gcgcctgttc gaagcgcacc agattgacgt cgagctcgtc gagccgctca gccgtctcgg
      601 tcaggaattc cgccagtaaa tcgtccacga ctcgcccttg gcgcgaatca gggtgattcg
      661 cgccttataa ccctgcagac aaggtcggaa ttgtcggcga attctgttta cgcttcgttt
      721 accagccggg gcgatttcgc cgaaatcggc gaattcctga agctcaggcg ccctttagag
      781 tgacctcatc gtcctcagcc tgagcggtca ggctgagccc tgccgcggtg gccacccggc
      841 ttgcgtaata gggctggatc gagtgggcgt cgatttcgac cccattgctc ccggacaata
      901 gcgccgtaac ctgctcgttg aggcgcgctg ccttgccctt ggcccgaact gtgaaggaaa
      961 ccgcctccgg cgggttgccc gcgatctcga cgctcaccgt gccgccgcgc ggcagggcca
     1021 cgacgccgag cgccacaaga ttgagcagcg ccttgacctg atttttgggc aaggtgacca
     1081 caggcccttc ccagatcacc tcatgcttgc cggttcccgc cacgaattcg cgggagactt
     1141 tctccgcgtc gcgaagatcg atctccgccc ccgcggaccc cgcggcgcca aaggccagac
     1201 gcgcgaattg cagcttcgcg gatgcctgcg ttgcgctctt gcggatcagg tccatggccg
     1261 cttcccgcat gtcagcatcc tgctcctcgc caagcatctc caacccgttg gagattgccg
     1321 caacgggatt gatgacgtcg tgacagatgc ggctggagac gagcgccgca agctcaagat
     1381 cggtgagccc cgtcggctct gtcatgaaat tctcccttcg cttccgcggc aatccactga
     1441 gatgacgtgg cggccaccca aatttttggt acaggagggt gcaatcggcg taacgacagt
     1501 acgcactcgg cgtaataacg aatcagcacc ctttggagca tcttgccttg tcagcaccaa
     1561 gacgcacaac cgccctcacc tcccaggccg gaaccgattt tgatctaggc caggcagcgg
     1621 ccgtcctcac ggatgcagcc gccaaggcgg gtgcggcgat catggcgcac tatgccggtc
     1681 ccgtggaggt cgaaacgaag gacgatgagt ctccagtgac caaagcggat cgcgacgcgg
     1741 aagcggcgat cctcccggtc ctggcggcgc tggccccttc catatccgtg gtctccgagg
     1801 agaccgcagc ggaccggaca gaagcgctcg ggcaacgctt cttcctcgtc gacccgcttg
     1861 acggcaccaa ggagttcatc aagaagcgct cggactttac ggtcaatgtg gcgctgatcg
     1921 aagaggggcg cccccgcttt gggctcgtct atgccccggc ccgatcgctg ttggccgtca
     1981 cgaccgccga cggcgtggtg gtcgaggccg aaatggcacc cagcgctgcc ggcgccgatc
     2041 tcgacgccct cccgcaaaag cgtcttcatg cccgcgcggg cgaccccaag gccctgacgg
     2101 cgctcgtgag cctttcgcac ctcgacccca agaccgaggc attcctggaa ggcctggata
     2161 tcgccgagcg gtccagcgcc ggctcttcgg tgaaattctt ggagatcgcg agagggaacg
     2221 cggacgtcta cccgcgcttc ggcccgacca tggagtggga caccgccgcc ggccaagcgg
     2281 ttctggaggc ctccggcggc cgggttgtcg atatggaggg caaccgcttc ggctacggca
     2341 agacggaaga tggcctacga aattcaggct tcgtcgcctg ggggcgcacg atcttaacgc
     2401 cccagtaacc cacccaccgt ttggtaactg tttcggtcaa caattggtcg cggacagtat
     2461 ttgtcccgcc cgccggcagg ggcgccggtt tcagtctcga gggtcactcg tcatgcgtat
     2521 cagcaccctt acgattgccg cttcgtcggc aatccttctg acttgcttct cactcaccgc
     2581 atcggcccag gatgaccaga tggtcattac gccggagtcg tatgcggaca tgatcgacgg
     2641 tcgagaaccg agccagctac cgccgccgcg ctcaagcggt gcggaaagtg ccggcactcc
     2701 cgccacctat ccgcaagggg gcgtcactga gacctatcag ccctccaacg ggtacgcctc
     2761 cgagacgctg cccggcagcg atgacgatac cttctccgtg gaggaaatct caggcgcggg
     2821 acaccgcttc ttcggcaagg tctccgtggg cttcgccaaa gcggtcgaat acgcctttca
     2881 gaaatccggg cgcccaaacg gctatatctt gggtgaagag gccggcgggg ccttcgtggc
     2941 cgggctgcgc tacggcgagg gcatactcta caccaaagac gcaggccagc accgcgtctt
     3001 ctggcaaggg ccttccgttg gctatgattt tggcgccgca ggctccaaga ccatggtcct
     3061 ggtctacaat ctcagcaacc cggcccagat ctacgacaga ttcgccgggg tcgacggctc
     3121 cgcctatttc ataggcggcg tgggcgtcac ctatctggag ggggacaagc tggttctggc
     3181 cccaattcgc tccggcgtcg gccttaggtt gggcgcaaat atcggctacc tcaaatatac
     3241 ccgccagccc acctggaatc cgttctagat tggcgctagg aaagcttagg aagatgggct
     3301 cgcgggggcg tttgatcttg gcgggcctgc ttgagcttgc ctaaaatgcg ccggtagaga
     3361 cgcttgggcg ctagtttcgg ggaaccctgg ttttgatgat cgagtggttc atgttcgggg
     3421 cgttgggctt cttcttggga tgcctgctcg ccctcatgct tgctcctccc ttgtggaacc
     3481 gtgctgtcaa gctcaccacg cgtcggcttg aggcgacgat gccattgacg ctcgccgata
     3541 ttcaggccga caaggaccag ctgcgcgctg agttcgccat tgagctgcgc aaggtcgaag
     3601 tcgccctcga caagtccaaa gagaaggccg cgcgcgagct gatcgaggcc aataagcgcc
     3661 gggtcgaaat ccaggaactc aataccgaga ttgccggcgt caaaggcaag ctcaccgaaa
     3721 gccagaacgc caatcgcgtc ctccagcaga cgatcaagcg gcgcctgccg gatctcgaca
     3781 cacggctcaa ggcagccaag aaggcgctgg ctgatcttga aaacgtcaac gccgagctgc
     3841 gcaacaccgt cgcctcccag tcagaagcgc tgaagattgc ccgcagcacg gttcaaaatc
     3901 agcgcgccga cattgaccgg ttgcgcttgt cgctcgaaag cggcagcgct ggcagtatgc
     3961 gcggatcgtc gaagtccgat gggcgggcgg tcgccgaaag ccagcgtctc gctgccgaac
     4021 tgtcgcgggc cacggaagaa ctcgagcgcg ccaagacggg cgccgaggaa atcgccttct
     4081 tgcgccgcga gttgaaccgc ctcgcctcgc acatcatgac cgccgcacga aaccaggcac
     4141 aacacgctca gcctgccgcg gccgcacaag agcacgcgga atacgcgcag agcgatacca
     4201 ccagcgaggt cacgaccgag gatgccgctt ggcacgccga ggctggaaac ggcgaggccg
     4261 ccgagcctat ggccgaggag gcacccgaag agatcgtggc cgccgagggc gagcatatgg
     4321 aatatgccga ggctgaagcc gaagtagagg ttgaggccga agtcgaggtt gaagctgaag
     4381 cagaagccga gatcgagcac gaagttaaag ccgaagccga ggttgagtct gtggaacagg
     4441 ctcggtttga ggacgcggtt gagtttgaag aggccgatcc ggagacccat gccgagatcg
     4501 aacctgacga gtacgaagac gtgccccatg aagggcacga cgaagaggtg tacgccgaag
     4561 aagacgcgga cgaggacgag gacgagcagc ggaccagcct cgcgcaacgc tttgccgccc
     4621 gcagatccaa gcgcgaaaaa ggcggcagcc tgaccagccg gctgcgcggc gttgttgcca
     4681 aggcgcctga aacgcgggcc taagagtcag gtctcaagaa aactccgctc cccatcctgc
     4741 aatctgagcg cagtcaatcg gccgctgaat accgcgccgg tgtcgatgcc gatgcgattg
     4801 ggccgcatga cgggttcaag cgcgggcgtg tgcccatgca ccacgacctt gccgaagctt
     4861 ttcgtcgacc cgagaaactc atcccggatc cagatcaggt ctgtctcgct ctgtgcctcg
     4921 agcggaacac ccggccgtac gcctgcatgg acgaagagat agtcgtctgc ggggatcagg
     4981 agatccaagc cctgcaagaa atggagatgc gttgcgggca acgctgacgc aaaggcctcg
     5041 cgccacgcct cgggtgccgc acgcacttcc tttagccagc cgacatcgac cccataggac
     5101 gccatggtct cctcgccgcc gttatagcgc cattgatcga gccgacttgc atcggcgaga
     5161 aaaccgagca gaagcgcttc gtggttgcct ttgagaaaat gcgcggagaa atctgatggc
     5221 aagccgtgga gcaggatctc gacgacgccg cggctatccg gaccacggtc cacgtagtcg
     5281 ccaagaaaaa tcagccgctt cttggcagcc aaacgcatcg cggcatccgc ctcgatcttg
     5341 agaagaagct ctgccaagag gtcggaacgg ccgtgaatat cgcccacggc ataaatcagc
     5401 tcgccctttg gcacggaagc aaactctccc gattaggcag cgcccgatgc gacgagttcc
     5461 cgatagagcg gtgccggaaa gctgaacacc gtgctcacgc aattcgattt gagcccgatg
     5521 aacaggacca tcttcccgtc gcgaagctta tcgtcttcgg tgatgacgta cagctcctcg
     5581 acgcgaagcg gcggcaagcc cttcaacaag gccgcccgct cctcgaacgc cttgaggctc
     5641 tcgccttcga ggtgatcgaa cttcgcgtgc acccgcgcga tggcgctgcg gatctcgctc
     5701 ttcgacttgc aaggaaccgc cggggcctgc ggccccgcgc cgttatagta agtcgtgccg
     5761 taggtctgca cgatcggacc aagcctctcg gagtaggccc gaaagcccac gaaccccaac
     5821 agcgtgaccg cgatcgcaag accagttgca atcttcgacc agagtggcat ggccaaatct
     5881 cctctaaaga gagggcctcc aatcgaacca aggacgcagc gataaggcgt tacgccctaa
     5941 atctccgcgt cgacctcaca gcgtaccgtg tcccggtggc cgcattcggg catcttccac
     6001 gcgatgacgt cgcccgtccg cgtatcgacg atcggcacag ggcatgcgac acgccagaac
     6061 cggtactttg aaccgcggtg actggcgtcc cactgggtac ccagcatgat acccgatctt
     6121 tggcaccgct gcaggttaaa cggctgccgg gcggcgccca aggcttccgc ccgatcgtag
     6181 agctgaacct cctgccgccg gcacaccatc atggagtgct cggtggccca ttcgaggttc
     6241 tcgtagcctg tgtacttggc gttgtgatct tcaggccccg cgctgacggt cttacagacg
     6301 atcaggacga cggcggctgg catggggaca ggcatggctc aagccccccg cgcgaggttg
     6361 gcgccctgaa atggagcgtt gagcttaggt agcagtgccg ttggcggctg tgccgtcacg
     6421 gccagggtaa gagtagtcgg catcttggcc ccctctctat agggttgcac taacgccact
     6481 ccctgagatc ctttacggac cgtggtacct ccctaactcc ctagaccggt tggaacttgc
     6541 gttcgcctca tcaggctgat gctgcactgc agcatcccgc cggatgtgtt ccacagttaa
     6601 gtatgcactc agccagtttc acgccaggta aacgccctgc ggctgagtgc cactaagtcc
     6661 gtggattcgc gcttcatttg cgttcccttt tccggccaga aggctggaga cgccgcgctc
     6721 gagcgctgat cagccttcgc aatccaatgc tgcaccgcaa aaaactgatt cgcaagactg
     6781 acataagcct acgccgatcg atcccccgcc gatagagcag agaagcaaca gaagtcagag
     6841 aattgccaaa gccttggcca gctgccatgg ccctttgcgc aaggattggg caggtgcaac
     6901 ccatgagttt tcccgagctc ctgtccatga actgcctcat gggcggcatg ctgccggtct
     6961 ccgtgctc
//
LOCUS       DBNHVU010000151         5014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_278747, whole genome shotgun sequence.
ACCESSION   DBNHVU010000151 DBNHVU010000000
VERSION     DBNHVU010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_278747"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /gene="hisD"
                     /locus_tag="AC16Y7_06095"
     CDS             complement(<1..743)
                     /gene="hisD"
                     /locus_tag="AC16Y7_06095"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR5708987.1"
                     /translation="MAQTLNTRDDGFAARFEALLGMKRESSTDVNTVVAAIIARVRAE
                     GDKALIDFTHELDRLDLTKTGIAVSAEEIAGAKAKVDAETFQALELAHARILDHHSRQ
                     RPQDETYTDEAGVELGQRWTPVASAGLYVPGGTASYPSSVLMNAVPAKVAGVERLVMV
                     VPSPDGVLNPLVLVAAALAGVDEIYRVGGAQAIAALAYGTETIAPVATIVGPGNAYVA
                     AAKRQVFGQVGIDMVAGPSEVVVIADADN"
     gene            complement(756..1232)
                     /locus_tag="AC16Y7_06100"
     CDS             complement(756..1232)
                     /locus_tag="AC16Y7_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HIR5708988.1"
                     /translation="MTLLKLLALDEEDLQVVSSHLQDAVVRVGDMVFLPSQKRFVAIV
                     NRFDWESATGKDTKNYKRRRTGLRFDRVLSAKHKELRPEKPDRVLSLLAIQFEAGEAP
                     SGTVTLTFSGGVIIQLEVECVEAELRDLGPSWVTRNKPDHPGDAAADTESSGASGA"
     gene            complement(1306..2595)
                     /gene="murA"
                     /locus_tag="AC16Y7_06105"
     CDS             complement(1306..2595)
                     /gene="murA"
                     /locus_tag="AC16Y7_06105"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR5708989.1"
                     /translation="MDRICIVGGEPLKGTIPISGAKNAALPLMIASLLTSETLTLHGM
                     PRLADVTLLGRILGNHGVDITIAGKRAGQSTSDGQTFKLTAAEIVDTTAPYEMVARMR
                     ASFWVLAPILARCGEARVSLPGGCAIGTRPVDLHLLALEALGADIELEGGYVIAKAPK
                     GLTGNRIPLGMVSVGATHNALMAAVLAKGETVIENAAREPEVGDLATCLTEMGAKIEG
                     IGTSTLHIQGVPSLHAAEHDVLPDRIETGTYAMAAAITGGDVFLKGARPELLQEALVT
                     LRTTGVDVLEEEGGIRVTRNGHGLDAVTVETQPFPGFPTDLQAQLMALMCTAQGVSEV
                     RETIFENRFMHVQELARLGARIDLKGDTALVHGVPGLRGAQVMATDLRASVSLIIAGL
                     AAQGETTIGRVYHLDRGFDRLEEKLRKCGAQIERYSG"
     gene            3065..3139
                     /locus_tag="AC16Y7_06110"
     tRNA            3065..3139
                     /locus_tag="AC16Y7_06110"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3097..3099,aa:Thr,seq:ggt)
     gene            3993..4211
                     /locus_tag="AC16Y7_06115"
     CDS             3993..4211
                     /locus_tag="AC16Y7_06115"
                     /inference="COORDINATES: protein motif:HMM:NF018595.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HIR5708990.1"
                     /translation="MPKQKYENNDLTVFYDPEVCIHAGDCVKGLPTVFDIAKKPWINV
                     EGAPTEEIVEQIKQCPSGALSYKLRKDT"
     gene            4256..4534
                     /locus_tag="AC16Y7_06120"
     CDS             4256..4534
                     /locus_tag="AC16Y7_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708991.1"
                     /translation="MTSRRFPAPWQVEQTPGGYKVLDANGQALAYVYARETRQQADIA
                     QVLTFDEARRDAVMGRLALIGGKRGAVRTLTLKAFSEDEYHKIVRSLD"
     gene            complement(4552..4758)
                     /locus_tag="AC16Y7_06125"
     CDS             complement(4552..4758)
                     /locus_tag="AC16Y7_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HIR5708992.1"
                     /translation="MQDNVYKVIHLIGTSSKSWEEAGKLAVEKAAKSLRDLRVAEVVE
                     QDMVVKDGKVEAYRTKVRISFKYE"
BASE COUNT          919 a         1583 c         1536 g          976 t
ORIGIN      
        1 tcgttgtccg catcggcaat gaccacgacc tcggacggcc cggccaccat gtcgataccg
       61 acctggccga acacttggcg cttggcggcg gcgacataag cgttgcccgg accgacgatg
      121 gtcgccacag gagcaatcgt ctcggtgccg taagcgagcg cggcaatcgc ctgggccccg
      181 cccacgcggt aaatctcatc gacgccggca agcgcggccg cgaccagcac cagcgggttc
      241 agcacgccgt ccggtgacgg caccaccatg acgaggcgct ccacgccggc gactttggcc
      301 ggcacggcgt tcatcaggac ggagctcgga tagcttgcgg tgccgccggg cacgtaaagg
      361 ccggccgagg cgaccggcgt ccagcgctgg ccgagctcca cgcccgcctc atcggtatag
      421 gtctcgtctt gcggccgctg gcgggagtga tggtcgagaa tgcgcgcatg ggcgagttcg
      481 agcgcctgaa acgtctcggc gtccacttta gccttcgcgc ccgcgatttc ttcggccgag
      541 acggcaatgc ccgtcttggt cagatcgaga cgatccaact cgtgcgtgaa gtcgatcagc
      601 gccttatcgc cttcggcccg gacgcgcgcg atgatcgcgg ccaccacagt gttcacgtcg
      661 gtggacgatt cccgcttcat gccgagcagc gcttcaaagc gtgccgcgaa gccgtcgtct
      721 cgcgtattaa gagtctgcgc catgcgttgc cgtcctcacg cgccggatgc gccggaactt
      781 tcagtatccg cggcggcatc gccgggatga tccggcttgt tgcgcgtcac ccaagagggc
      841 ccgaggtcgc gcagttccgc ctcgacgcac tcgacttcga gctggatgat gacgccgccg
      901 gagaaggtca gcgtgaccgt gccgctcggt gcctcgcccg cctcgaattg aatggcgagc
      961 agcgacagca cgcggtccgg cttctcgggc ctgagctcct tgtgcttggc gctcaggacg
     1021 cggtcgaagc gaagccccgt gcggcggcgc ttatagttct tcgtgtcctt gccggtcgcg
     1081 ctttcccagt cgaaccggtt gacgatggcg acgaaccgct tctgcgacgg cagaaagacc
     1141 atatcgccca cgcgcaccac cgcatcttgg agatgcgacg acacgacctg gaggtcttct
     1201 tcatcgaggg cgagcagttt cagcaatgtc atggcgaatt tggtctttct ggcaggggcg
     1261 tatcacgctt tttcgggcgt gcggaaggaa taacgcaaga gatacctagc cggaataccg
     1321 ctcgatctgg gcgccgcatt tccgcaattt ctcctcgagc cgatcaaatc cccggtcgag
     1381 gtggtagacg cggccgatcg tggtttctcc ttgcgccgcc agcccggcga taatgagcga
     1441 caccgaggcc cgcaggtccg tggccatgac ttgcgcgccg cgtaagcccg gcaccccgtg
     1501 aaccaaggcg gtatcgccct tcaggtcgat ccgggcgccg agccgcgcca gttcctgcac
     1561 atgcatgaag cgattctcga aaatggtctc gcgcacctcc gaaacgccct gcgcggtgca
     1621 catcagcgcc atgagctggg cctgcaggtc cgtcgggaac ccggggaagg gctgggtctc
     1681 aacggtgacg gcatcgaggc cgtggccgtt gcgcgtcacg cgaatgccgc cctcctcctc
     1741 aagaacatcc acgcccgtgg tccgcaacgt gaccagcgcc tcttgcagaa gctcgggccg
     1801 ggcgcctttc aggaatacat cgccgccggt gatggccgcg gccatggcgt aggtgccggt
     1861 ctcgatccgg tccggcagca cgtcgtgctc ggcggcatgc agcgagggca cgccttgaat
     1921 atgcagcgtc gaggtgccga tgccttcgat cttcgcgccc atctccgtga ggcaggtggc
     1981 cagatcgccg acctcgggct cgcgggcggc gttctcgatc accgtctcgc cctttgccag
     2041 cacggcggcc atcagagcgt tatgggtggc gcccaccgaa accatgccca gcgggatgcg
     2101 gttgcccgtc agccccttgg gcgccttggc gatgacgtaa ccgccctcga gctcgatatc
     2161 ggcgccgagc gcttccaacg ccagaaggtg aagatccacg gggcgggtgc cgatggcaca
     2221 gccgccgggc agcgacacgc gggcctcgcc gcagcgcgcc aggatcggcg ccagtaccca
     2281 gaagctggcc cgcatgcggg ccaccatctc gtacggcgcg gtggtgtcga cgatttcggc
     2341 ggcggtgagc ttaaaggtct ggccgtcgct cgtgctttgg ccggcgcgct tgccggcgat
     2401 ggtgatgtcg acgccgtgat tgccgagaat gcggccgagc agggtcacgt cagcgaggcg
     2461 tggcatgcca tgcagcgtca gcgtctcgct ggtcaaaagg ctggcgatca tcaggggcag
     2521 cgcggcgttc ttggcgccgc tgatggggat cgtgcccttg agcggctcac cgccgacaat
     2581 acaaatacgg tccatgatcg acgtgtagct ccctcacgca acgctccagc gaatgcttcc
     2641 gcgcctcgat ttcccgatat tccgcccccc ggcatggacg gcaagcggcc agtctaccct
     2701 ccccgcctca ggtctgtcgc ccctcttcgg acgggggggc gcgggacctt tgtggcgcgg
     2761 gccttctcct tgcgtttcag caggttggca cgaagcgcct gcgccagccg ggcctggcgg
     2821 tcttgcccct gggccggctt gtccggcgcg cgcgtcttgt cgtcctcgcc agccatatcg
     2881 ctcgcaattt tgctgatctt gaggccggtt ttataacatg gaagccgagc ggttaagccc
     2941 ggcggtatgt ctcttgtcgc gtcttgcgcg gcagagcccg ctatggcagt ttgcgcgccg
     3001 cacggtccaa ggcgccctta ttcttgagga cacgcgcccc gtgccgaccc accggtcaca
     3061 acacgccgcc gtagctcagt ggtagagcgc gtcattggta atgacgaggt cgatagttcg
     3121 atcctatccg gcggcaccat tattcaatga gttagacgaa cctcttgcgc cggccttcat
     3181 gccagtaagc acatagtaag caccgcgaac gcattcgaag caccagagcg gcgcgtaaga
     3241 ccgtgggcga aaagcggcgc cctcgaggtc agcaaaagta agcatccgtc gcacctctag
     3301 agaccctctt ttaatctccg caatgccgac accatacggg ggagccaatg agggagggtc
     3361 ctatgaccga cgacgacaag gcacatcaga caacgcacgg ttcgatgcgg tcacacacac
     3421 cttggaagac tgccaccata gtccttgcgg tcatactggg cttggtcctg atctgcgccg
     3481 tagtcgtggt agcagacctc acaggcttgg cattgtcgct gctttaacga agccgaacac
     3541 gccacttcat tcgctttagc gttggcgcat ggtcgtgcgc tgctacactc agcctgcggg
     3601 ggaagccatt tgagggaggc ttcatgacat cgctatacca atcaatatct gttgcggttt
     3661 gtgttcttgc tgccccgtgg cggtggcggc gttcctgcgg tccaatcagc tatgcagttc
     3721 gcgacgtaag cttctcgttc cctggcatcg agcgccggaa ggacacgatt ggcatgatac
     3781 aggcagagac cttgaacccg ttgtggagcg gccaatgctg gcgttgaggc gagcacaatg
     3841 ctcgcaacga atagctttag aacgttgagc gtcatacata cctccaagtc cgctgtgcct
     3901 gcaaaaagac ctaccgtaag cctctttgct gcgagtgact ataggctgct atgctcggcc
     3961 ttcgggggga gccaattgag ggaggattcg tgatgcctaa gcaaaagtac gagaacaacg
     4021 acctcactgt cttctacgac ccggaagttt gcatacatgc cggagactgc gtgaagggtc
     4081 ttccgactgt ctttgatata gcgaagaagc catggatcaa tgtagagggc gcgcctacgg
     4141 aagagattgt tgagcagatt aagcagtgcc cctccggcgc gcttagctac aaactacgga
     4201 aagacacgta aagtacatac accagcgggg ggaaagccat ttgagggagc tttccatgac
     4261 atcacgccgt tttccggctc cctggcaagt cgagcagacg ccgggtggct acaaggttct
     4321 cgacgctaac ggacaagcgc tggcctatgt ctatgcgcgg gaaacgaggc aacaggctga
     4381 catagcgcag gtgctgacgt tcgatgaagc gcggcgcgat gcagtcatgg gccggctcgc
     4441 acttatcggc ggcaaaagag gtgctgtgcg cacgctcacg ctaaaagcct tctccgagga
     4501 cgaatatcac aagatagtcc gttcgctcga ttagcacacc gttcggcggt gctattcgta
     4561 cttgaacgaa attctgactt ttgttcggta ggcttcaact ttgccgtcct tgacgaccat
     4621 atcttgctcc acaacttcgg ccacccgtaa gtcgcgtaag ctctttgcgg ccttctcaac
     4681 ggccaatttg ccagcttcct cccaagactt tgagcttgtg ccgatgagat ggatgacttt
     4741 gtagacattg tcctgcatag cgttctctcc cttcgcccgg catcgcagag gttctgtgcc
     4801 agccccggca acgtcgcagg gcctgttatc gtcatgtgcc gagagcaagt aagcatgcgc
     4861 ctgctgaggg tctaaagcca ggcacctatc tcgcaatgca gcaagcttct gcgcggcgca
     4921 tcattcgagg aaccttccgg ctgtccatcg tcgccgctgt tgccggtgcc gatgagcccc
     4981 tcacctctct cctcgcacac aggcccaagc tctc
//
LOCUS       DBNHVU010000152         5043 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_500705, whole genome shotgun sequence.
ACCESSION   DBNHVU010000152 DBNHVU010000000
VERSION     DBNHVU010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_500705"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="AC16Y7_06130"
     CDS             complement(<1..969)
                     /locus_tag="AC16Y7_06130"
                     /inference="COORDINATES: protein motif:HMM:NF020650.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5708993.1"
                     /translation="MAHADAAPLPITVSAYRADRTAALADGRTGIEGCAVTFEEDAIG
                     DMNTHAFDGPGTREVTEIGLHPFMLAYANDGFRDYMLLPVFLLRQFRHKSVFIRTNRG
                     IERPEDLAGRRIGTPGYSSTSLTWIRGIFEDEYGVKPPEIEWVVSAADSSAEAAGKAS
                     AQENRLPQGVNITTGPEGLDDSDLLEQGIVDALFHAVEPRCYRQGNPLVGRLFPDHRE
                     VEQEYFKRTGIFPIMHAVAIRRDVAAKHSWFPKAVFDGFVEAKTEAYRQMARLGWATN
                     MLPWFGQELEDTKALMGNNFYSYGIAPNRKALETLFRYSYTQGLATR"
     gene            complement(1041..2714)
                     /locus_tag="AC16Y7_06135"
     CDS             complement(1041..2714)
                     /locus_tag="AC16Y7_06135"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR5708994.1"
                     /translation="MAARADDAIVHDAEYYVLEAQHGEEWAAEDVALRATLDELKKKH
                     GRPPNLIHIMWDDTAFGDVGIPAIQKVRGLTTPSINRLAEEGILFTRMYTEVGCTPSR
                     AAVVTGRHPIRNGMYNIGMLLESHGMRGEEVTMAEVLSKAGYNTAFHGKWHLGDIEES
                     YPHKQGFDEAFFTGYNQILSLNTKLAEGANASIGLHESILPLDPYKLDDSFVTKGWVM
                     IAEGKKGEKAQQWGDNSHDTYVKIDPEAQKRTLDFIEKSVKEDKPFYIANWPNLTSFI
                     PNPKKCAVARSLLQDGLQCNIDPFIAKLIAKLEELEIADNTLIVAMADNGPMAHNPPP
                     GLGMAETIYRGGKGDFLEGGVRVPAMAWWPGVIKPGQQVGDIVHEVDLFTTFANLADA
                     TQYIPTDRVIDGVDQTALLFEGDTNSRRDYVFIYAGPKLGATVKGNYKRHWISSDPVG
                     EASGIPAAFYFLPADPREKTPMLVNLIHLKSPFNRMRLRHELWMKKYPNNKEKFGVPW
                     TGIANASPEIEALADPKMDLSKLPFDPLEYVEHLDELPFDPNMDPSLGQ"
     gene            3061..4788
                     /locus_tag="AC16Y7_06140"
     CDS             3061..4788
                     /locus_tag="AC16Y7_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR5708995.1"
                     /translation="MPRKSLSALFSTALLVVGIGVSPLHAEEIVHDAEFYILKAQNGG
                     NWAEQDKKLDAKLAALEEKFGTPPNIVYILWDDTAFGAAGFPALQKNFGYETPNLNKM
                     AEEGINFTRMYTEPSCTPTRAAFLTGRHPVRHGMGVVGMPHEFSGLRAEEVTIAEVLS
                     DAGYATGFFGKGHLGDIEESYLHNQGFDEALFTPMNQITSLYNPQGNAVNAVLGMFPE
                     IYPPDPYQLDSPGLVPEGWVMNIEGKKGEQGREWCGTSNECYGKLDSEAEKRTLAFIR
                     KNAEAKKPFFVSYWPNFLNFMEVQMPKKSVAGLMVADSFPKLDNFVGSLMDELKQLGI
                     AENTLVIAMADNGPMVHSPPAGWGMLPMLYRGGKGDFTEGGVRVPAFAWWPGMIEEGQ
                     VVADIVHVTDLYTTFARLGGATGNIPTDRVVDGLDQTAVLLDGDTNGRRDYVFIYAGH
                     QLGATVKGRYKRHWIGAGDVAASGMPEAYYDLYMDPREENPQLVPLIHTQGQFNHMRA
                     WHELFKKKYPDVENAKGIPYTGLSNARPETKAIAERVKATVDAMPFDVEEYLEFDLPG
                     SDAVGDWGR"
     gene            complement(4814..>5043)
                     /locus_tag="AC16Y7_06145"
     CDS             complement(4814..>5043)
                     /locus_tag="AC16Y7_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR5708996.1"
                     /translation="MSASFTNQVLAQIELWQHAGKYKNEVYVLPKHLDEKVAQLHLAK
                     LGAQLTKLSEEQASYIGVKPEGPFKPEHYRY"
BASE COUNT          985 a         1467 c         1526 g         1065 t
ORIGIN      
        1 gcgggtcgca agcccttgcg tgtaggaata gcggaacagt gtctcgagcg ctttgcggtt
       61 gggagcgatg ccgtaggaat agaaattgtt gcccatcagg gcttttgtgt cctcaagttc
      121 ttgtccgaac cacggaagca tgttcgtggc ccatcctagg cgcgccatct gccggtacgc
      181 ttcggtcttt gcttcaacga aaccgtcgaa cacggccttc ggaaaccagg agtgcttcgc
      241 agcgacatcg cggcggatcg cgacggcgtg catgatggga aagattcccg tgcgcttgaa
      301 gtattcctgt tcgacttccc gatggtcggg aaagagccgc ccgactagcg gatttccttg
      361 cctgtagcag cgcggctcca ccgcgtgaaa cagcgcatcg acaattcctt gttcgaggag
      421 atcgctgtcg tcgaggccct caggacctgt ggtgatgttg acgccttggg gcaggcggtt
      481 ctcttgtgcc gaagccttgc ccgcagcttc tgccgaggag tctgctgccg aaaccaccca
      541 ctcgatctcg ggcggcttca cgccgtattc gtcttcgaat atcccgcgga tccaggtgag
      601 tgaggttgag gaatagcccg gcgttccgat gcggcgtccg gctaagtcct ctgggcgctc
      661 gatgccccgg ttggtgcgga tgaacacgct cttatgccgg aactgacgaa gcagaaagac
      721 cggcagtagc atgtagtcgc ggaacccatc attcgcatag gccagcatga agggatgaag
      781 cccgatctcc gtgacttccc gtgtgccggg accatcgaag gcatgggtgt tcatatcccc
      841 gatggcgtcc tcctcgaatg tcacagcgca accttcgatc ccggttcggc catcggcaag
      901 cgcggcagtg cggtctgcgc gataggccga aacggtgatc ggtagcggcg cggcgtccgc
      961 gtgagccata gaagcacgaa aaagaagggg agcagccgcg agggcggccg ctccggtcag
     1021 aaccttccgt ctcgtcggcc tcactgcccg aggctcgggt ccatgttagg gtcgaacggc
     1081 agctcgtcga gatgctctac atattctagc ggatcgaaag gcagcttgga gagatccatc
     1141 tttggatccg cgagcgcttc gatttcaggc gacgcgttcg caatcccggt ccagggcacg
     1201 ccgaacttct ctttgttgtt cggatatttt ttcatccaaa gttcgtgccg caggcgcatc
     1261 cggttgaacg ggctcttcag gtgaatgagg ttcaccagca tcggcgtctt ctcccgcgga
     1321 tcggccggca ggaagtagaa ggcagcaggg atgccgcttg cctcgccgac gggatcggac
     1381 gagatccaat ggcgcttgta attgcctttt actgtcgcgc cgagcttggg cccggcatag
     1441 ataaagacat agtcgcgccg gctgtttgta tcgccctcga acagaagcgc cgtctggtcg
     1501 acgccgtcga tgacgcggtc ggtcgggata tactgcgtgg cgtccgccaa attggcgaac
     1561 gtcgtgaaca gatcgacttc gtggacgatg tcaccgacct gttgaccggg cttgatcacc
     1621 cccggccacc acgccatcgc ggggacgcgc acgccgcctt ccagaaagtc acccttgccg
     1681 ccgcggtaga tggtttcggc catgccgagc ccgggaggcg ggttgtgggc catggggccg
     1741 ttgtcggcca tcgccacgat cagtgtgtta tcggcgatct cgagctcttc gagcttggca
     1801 atcagcttcg cgatgaaggg gtcgatgttg cactggagcc cgtcctgcaa aaggcttcgg
     1861 gcgaccgcgc attttttcgg attgggaatg aagctggtca ggttcggcca gttcgcgatg
     1921 tagaagggct tgtcctcctt cacgctcttt tcaatgaagt cgagcgtgcg cttctgcgct
     1981 tccggatcga tctttacata ggtgtcgtgg gagttgtcgc cccattgctg cgccttctcg
     2041 cccttcttgc cttcggcgat catcacccaa cccttggtca cgaagctgtc gtcgagcttg
     2101 taggggtcca gcggcagaat actttcgtgc aagccgatag aggcattggc gccttctgca
     2161 agcttggtgt ttagcgacag gatctgattg tagccggtga agaaggcctc atcgaaaccc
     2221 tgcttgtggg gatagctttc ttcgatatcg ccgagatgcc atttgccgtg gaaagcggtg
     2281 ttgtaaccag ccttagatag cacctcggcc atggtcactt cttcaccgcg catgccatgg
     2341 ctctccagga gcatgccgat attgtacatg ccgttgcgga tcggatgccg tccggtgacg
     2401 accgcggcac gactcggcgt acagccgacc tccgtgtaca tgcgggtgaa caggatgcct
     2461 tcttccgcta gccggttgat gctcggcgtg gtcaagccac gcactttctg gatcgcaggg
     2521 atgccgacat cgccgaaggc cgtgtcgtcc cacatgatgt ggattaggtt ggggggacgc
     2581 ccatgcttct tcttcagttc gtcgagcgtc gctcgtaacg cgacgtcctc ggcggcccac
     2641 tcctcgccgt gctgcgcttc gaggacgtag tactctgcgt cgtggacgat cgcgtcgtcg
     2701 gctcgagcgg ccaacggtgt cccgaaggct gcaactatgg cgatgccgcc agcaagaacg
     2761 attgatgcta aagtcgattg cctcatcacc gaacctccca ctctggacgc ctaggcctta
     2821 tagaaagaaa catggcaaag cctgtcattt ggggccgcga agacggcggg attgcacgct
     2881 ctaggcacaa ctctcgacgc gagttgcaac atcgggcccg aattaagcag ggtacctttc
     2941 acatattcga gacggattgg cctttcaact tttggcccat agcctcacct gaacgctctg
     3001 ttacgcttat taccaagacg ccgtctatgc gcggcggaac aatacatctg ggaggagcaa
     3061 atgccacgta agtctttgtc tgcactgttt tctacggctc tccttgtcgt tgggataggc
     3121 gtgtcgcccc ttcatgccga agagatcgtt catgatgccg agttctatat tctcaaggcc
     3181 caaaacggag ggaactgggc ggagcaggac aagaagctcg atgccaagct cgctgcgctc
     3241 gaagagaaat tcggcacgcc ccccaacatc gtctatatct tatgggacga cacggcgttc
     3301 ggtgcggccg gctttccggc gctgcaaaag aacttcggct acgagacacc caatctcaac
     3361 aagatggccg aagagggcat caacttcacg cggatgtaca ccgagccgtc ctgcacgccg
     3421 acccgagcgg cgttcttgac cggccggcat cccgtgcgtc acggcatggg cgtggtcggc
     3481 atgccgcatg agttttccgg attgcgggcg gaggaagtga ccatcgccga agtgctatcg
     3541 gacgcaggtt acgcgacagg cttcttcggc aaggggcatc tcggcgatat cgaggaaagc
     3601 tacctgcaca accaagggtt cgacgaagcg ctgttcacgc cgatgaacca gataacctcg
     3661 ctctataatc cgcaaggcaa cgcggtgaac gccgtactcg gtatgttccc ggagatctat
     3721 ccgccggacc cctaccagct cgacagtcct ggcctcgttc ctgaaggctg ggtcatgaac
     3781 attgaaggca agaagggcga gcaggggcgc gagtggtgcg gcacgtccaa tgagtgttac
     3841 gggaagctgg acagcgaagc tgagaaacgg actcttgcct tcattcgcaa aaacgccgaa
     3901 gcgaaaaagc ccttcttcgt ctcatactgg ccgaacttct tgaatttcat ggaggtccag
     3961 atgccgaaga agtcggtggc cggactgatg gtcgctgact cctttcccaa gctggacaac
     4021 tttgttggtt cgttgatgga cgaactcaag cagctcggca ttgctgagaa caccttggtc
     4081 attgccatgg ctgacaatgg ccccatggtg cacagcccac cggccggttg gggcatgctg
     4141 ccaatgctgt atcgcggcgg caagggtgac ttcaccgaag gcggcgtgcg tgttcccgcc
     4201 ttcgcttggt ggcccggtat gatcgaggaa ggccaagtcg tcgcggacat cgtccacgtc
     4261 accgacctgt acacgacctt cgcccggctg gggggcgcca ccggaaacat tccaacggat
     4321 cgtgtcgtcg atgggctcga ccagacggcc gttcttttag acggcgacac caatggccgg
     4381 cgcgactacg tgttcatcta tgccggtcac cagctcggcg ccacggtcaa gggacgctat
     4441 aagcgtcact ggataggcgc cggagatgtc gcggcgtccg gcatgccgga ggcctattac
     4501 gatctttaca tggacccgcg ggaggagaac ccgcagttgg ttccgctcat tcacacacaa
     4561 gggcagttca accacatgcg cgcctggcat gaactcttca agaagaagta cccggatgtc
     4621 gagaatgcca agggcattcc ctacaccggg ctctccaacg cgcggcccga gacgaaggcg
     4681 atcgccgaac gtgtaaaagc cacggtcgat gccatgccgt tcgacgtcga ggaatatctc
     4741 gaattcgatc tcccgggtag cgacgccgtt ggcgactggg gccgctaacg gagcgccccg
     4801 ccgactgacg cgcctagtag cggtagtgct ccggcttgaa cgggccttcg ggcttcacgc
     4861 cgatatagga ggcctgttcc tcgctgagct ttgtcagctg agcgccgagc ttggcgagat
     4921 gaagctgcgc gaccttttcg tccaagtgct tcggcagcac atagacctcg ttcttgtact
     4981 tgcccgcgtg ctgccacaac tcgatctgag cgagcacttg attggtaaag gaggcgctca
     5041 tca
//
LOCUS       DBNHVU010000153         9158 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1020632, whole genome shotgun sequence.
ACCESSION   DBNHVU010000153 DBNHVU010000000
VERSION     DBNHVU010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 9158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9158
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1020632"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1323)
                     /locus_tag="AC16Y7_06150"
     CDS             complement(<1..1323)
                     /locus_tag="AC16Y7_06150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR5708997.1"
                     /translation="MHGPLQETSDGEKTGGLERFRQALLDPAVSVAHYGAALGVMGVV
                     LTLAWLLRPLVQGEGSFLIFIPAVLFAAGFGGIGPGLLVTAITSVLGALLIGNGTVPA
                     PALLEPAVFAVVGIGISWFGELLRRTRMRSRRHATDLRAREAYIRSVLDAAPDAAVVI
                     DERGTIQSFSAAAERLFGYDKDEVTGRNVSVLMPAPFRQDHNGYIARYIATGEKRFIG
                     TDRVVTGERKDGSTFPMKLAVGEMRTGDGRFFTGFIRDLTERQQTEHQLQELQTELAR
                     LSRLTAMGEIASTLAHEINQPLSAISNYLQGCSRLLEPMEHPNTPKIRDALAETTKQT
                     LRAGQIIRQLREFVTHGETEKQPEDVNKLIEEASALGLVGAKDEGVRTIFRFDTDLGP
                     ALVEKVQIQQVFLNLMRNAIEAMEDSERKELCVSTSLAAHDKDEQGRPM"
     gene            1465..1734
                     /locus_tag="AC16Y7_06155"
     CDS             1465..1734
                     /locus_tag="AC16Y7_06155"
                     /inference="COORDINATES: protein motif:HMM:NF039524.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ElaB/YgaM/YqjD family protein"
                     /protein_id="HIR5708998.1"
                     /translation="MATTDAEMPDHKALNEDLQEIAEHIEALRSNLNALAQSVGATGS
                     HQAEALQAQAGQSLGAMEDAVRREPLKSLGIALGVGFLLGVVMGR"
     gene            complement(1763..2587)
                     /locus_tag="AC16Y7_06160"
     CDS             complement(1763..2587)
                     /locus_tag="AC16Y7_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5708999.1"
                     /translation="MKLVRILLPIGAGGASGACTEAAFRLAERFGAGLEVLHPCPPPV
                     QRLPYATELSPVYFEEMVDASRKQVDYEERKATDWFEEQLGAHPEVAAELVTVEGLVG
                     PAVAMRAKIADVTVLPSVADEEDELWSAARDAALFQSGRPILVVPDKAAGPLGETVVI
                     AWKDTVEAVRAVASAAPFLATANRVRLLCLDEGEESDGTASGMADYLTRAGYPVEIER
                     ASLEGRAVGEALLAATGKADLLVMGAYGRWRWREWIFGGATLHVLRNTHVAVLMSH"
     gene            2787..3164
                     /locus_tag="AC16Y7_06165"
     CDS             2787..3164
                     /locus_tag="AC16Y7_06165"
                     /inference="COORDINATES: protein motif:HMM:NF014534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043024 - ribosomal small subunit binding
                     [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HIR5709000.1"
                     /translation="MQLPLDISFRNMDPSPAVEARIREKAAKLERFHERIISCTVVVE
                     APHRHHHKGKIYSVHIDISVPGKDHLIVDRAKPADHAHEDVYVAVRDAFNAAVRQLED
                     YTRRMRGNVKAHTASRGEPSAAE"
     gene            complement(3175..4701)
                     /locus_tag="AC16Y7_06170"
     CDS             complement(3175..4701)
                     /locus_tag="AC16Y7_06170"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF017085.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050355 - inorganic triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HIR5709001.1"
                     /translation="MASCESQSSSNAKEIELKLEFDADAAPRILSHPIFEGPPPKTRE
                     LVSIYYDTGASDLRNAGVFLRVRSTGDGYVQTIKSAKSEGEFLERNEWECPVPTHEPN
                     LDAAEGTALARLLTPEVRADLKPRFHTRFRRKTYDVRHRGAEIELAVDQGEATAGVKA
                     APISELELELKAGAKAALFSLARELAETAPLNLAVKTKAERGFELLDDADHTVENARR
                     VVVTPDMTSAEAFRSIARNCLRQIVANAPAMREGRAEALHQMRVGLRRLRVAIALFGQ
                     MLGGDERKTIKDKLRWITQELGPARDLDVFAADVLEPMRAAYPGDREVEDTYRDFKER
                     HAAAYARATQSANSDRFRVALLDITAWVETGDWARKNRKLAKKPVRRHALKQLARLRR
                     GIKAKGRRLRDLSPAKRHRLRIRSKRLRYATEFFAATFPKKKSEERRNVSLAALEAIQ
                     DALGVLNDMATRKSLLATGQEDWRLRAPAIDIAEEEKWLKEAKRAYGRFAKAKAFWKA
                     "
     gene            complement(4917..6167)
                     /locus_tag="AC16Y7_06175"
     CDS             complement(4917..6167)
                     /locus_tag="AC16Y7_06175"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR5709002.1"
                     /translation="MHLDTMTMSVVNLSVTAIVAAMLLLTWGRAGWEQAGGPRFVGAW
                     GLAMLTHVAGAAIVLGAALANEVNLVTIGASIAILAVALKWDAAREYAEHERAPFWIM
                     FGPVGFIVLSHSGFVESLDARLIVGCIFFAAYNFAAALELRRIGDRQLCWPAVSLLVL
                     VGLSYLSWIPFILVVPIQTLGEAFTIAWLPAVALVTMLMRVALAFIVLSMAKEREEQE
                     QRRDALTDSLTGLPNRRALFEVADTVGERRVLGGTPVSVLIFDLDHFKDTNDSYGHSF
                     GDEVLKIFAMIAERDLKENAIVGRLGGEEFAAILPGIDAKRAVAAAERVRRAFSNAGA
                     FVNGLAVGATVSVGVASDTDVDADLSGLFRRADAALYVAKRAGRDRVAILESDENVFP
                     GTTVRSSPTRARKRPVPRRPLQTV"
     gene            complement(6289..6570)
                     /locus_tag="AC16Y7_06180"
     CDS             complement(6289..6570)
                     /locus_tag="AC16Y7_06180"
                     /inference="COORDINATES: protein motif:HMM:NF040427.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6455 family protein"
                     /protein_id="HIR5709003.1"
                     /translation="MKRPRQDLAGFHHQVLMDDMMQVVGVDLLDVIDVDGGQSYLRAR
                     SNCHACICKDGCRDWLTEARSELKNEPEEKQPQPFCPNAGLFEAVKRGD"
     gene            complement(6661..7194)
                     /gene="ppa"
                     /locus_tag="AC16Y7_06185"
     CDS             complement(6661..7194)
                     /gene="ppa"
                     /locus_tag="AC16Y7_06185"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HIR5709004.1"
                     /translation="MNLSQIPVGDNPPHEVNVIIEIPQGGTPVKYEVDKKSGALFVDR
                     FLHTSMMYPANYGFIPHTLSEDGDPVDCIVIAPTPVASGTVIRARPVGALLMEDEAGI
                     DEKIIAVPIDELHPFYKRVTSYRDLPEILVEQIGHFFEHYKDLEKGKWVKVMRWAEVG
                     EAAELIRTGMERAGTNA"
     gene            complement(7191..8300)
                     /gene="modC"
                     /locus_tag="AC16Y7_06190"
     CDS             complement(7191..8300)
                     /gene="modC"
                     /locus_tag="AC16Y7_06190"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HIR5709005.1"
                     /translation="MISVDVALKLDDFDLDAAFENEAGITCLFGRSGAGKSLTISLIA
                     GLARPDRGRIVLDGRTLVDIEAGTFVPPHRRQMGLVFQDARLFPHLSIKQNLLYGRKF
                     AGKVPEHVQLDKVVETLGIGHLLRRRPASLSGGEKQRVSIGRALLASPALLLMDEPLA
                     SLDTERKLEILPLIESLRDTFGIPIVYVSHAVDEVARLASRVVVLDAGRVVAAGPVEE
                     TLGPALRGIGISPLARSSVVRGAVAAVDGDYGLTEIAHPAGKIWLVGNAGPKGREVRV
                     AVKATDITLARDAGPNLSIRNVLAGTVEEVDTHGGAFAGVSIALDGHGHLFALATRKA
                     VDELAIRAGGRIFALIKTVALDERAGANMETLSEL"
     gene            8458..8754
                     /locus_tag="AC16Y7_06195"
     CDS             8458..8754
                     /locus_tag="AC16Y7_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709006.1"
                     /translation="MRPTFLAFLLTLSALAATGPATAEPDMDTVRCTAIRDAAQCVSR
                     PDCWYDAAGGNGCLKGPRPDENPCQVHGSKSVCNTSTLGCTWNEAESKCVRKAN"
     gene            8777..9145
                     /locus_tag="AC16Y7_06200"
     CDS             8777..9145
                     /locus_tag="AC16Y7_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIAA family protein"
                     /protein_id="HIR5709007.1"
                     /translation="MIELLKGFPDNVVALACHGHVTREDYDKVLVPALEKALRSNDKV
                     RVLYEIGGDFEGYDVSASWEDFKVGMEHLPRWERIAVISDIEWIKHTMQLFSFVMPGT
                     VRVYPTEEAAKARLWITQAA"
BASE COUNT         1752 a         2989 c         2820 g         1597 t
ORIGIN      
        1 catcggccta ccctgctcgt ccttgtcatg cgccgcaagg gacgtcgaaa cgcagagctc
       61 cttacgctcg ctgtcctcca tcgcttcgat ggcattgcgc atgaggttga ggaacacctg
      121 ctggatctga accttctcca ccaaagcggg gccgagatcc gtatcgaagc ggaagatggt
      181 ccttaccccc tcgtccttgg ccccgaccag accgagggca ctggcctcct cgatcagctt
      241 gttgacgtcc tccggctgct tctcggtttc gccatgggtc acgaactcgc ggagctggcg
      301 aataatctgt ccggcgcgca gcgtctgctt ggtggtttcg gcgagtgcgt cgcgaatctt
      361 cggcgtgttc ggatgctcca tgggctcgag caaacggcta cagccttgca gatagttgct
      421 gatggccgag agcggctgat tgatctcgtg cgccagcgtc gaggcgatct cgcccatggc
      481 agtcagccgc gacaggcgcg cgagctcggt ctgaagctct tgcagctgat gctcggtctg
      541 ctggcgctcc gtcaggtcgc ggatgaatcc cgtaaagaag cggccatcgc ctgtccgcat
      601 ctcacccacc gccagtttca tgggaaaggt ggaaccgtcc ttgcgctcgc cggttacgac
      661 gcggtcagtg ccgatgaagc gcttctcgcc ggtggcgatg tagcgcgcaa tgtagccgtt
      721 gtgatcttgg cggaagggag ccggcatcag gacgctgaca ttgcggcccg tgacctcatc
      781 tttgtcatat ccgaacaagc gctcggcggc ggcgctgaag gactgaatcg tccctctctc
      841 atcaatcacc accgctgcgt ccggcgccgc atccagaacc gaacggatat aggcctcgcg
      901 ggcgcgcaag tctgtcgcgt ggcggcggct gcgcatcctg gttctgcgga gcagttctcc
      961 aaaccaagaa atgccgatgc cgaccacggc gaacacggca ggctccaaca acgcaggagc
     1021 cggcactgtt ccgttcccga tgaggagcgc acccaagaca gaggtgatcg cagtgaccag
     1081 aagccctgga ccgatgccgc cgaacccggc cgcaaacagc acagcaggaa tgaagatgag
     1141 gaaggagccc tcaccttgaa ccagcggccg caacagccaa gcgagcgtga gcaccacgcc
     1201 catgacgcca agcgcggcac catagtgggc gacggagacg gccggatcga gcagcgcctg
     1261 acgaaagcgc tctagaccgc ccgttttttc accgtcggag gtctcctgga gaggcccatg
     1321 catgatcata ctcgcctcgg gcgacaagcc cggcccaaca gcgtcccaag ggggaacgtt
     1381 atcgcaattc tgtcagctct ctatagttag ctggatcgac gccgcaatgc agggctaaag
     1441 tcccttggcg cgaaggagga agccatggcc acgacagatg ccgaaatgcc agaccacaag
     1501 gccctgaacg aggaccttca ggagatcgcc gagcatatcg aggcgctccg ctccaacctc
     1561 aacgctttgg cacagtccgt tggcgccacc ggcagccatc aagccgaggc gttgcaggcg
     1621 caagccgggc agtccttggg cgccatggaa gacgccgtcc gacgcgaacc tttgaagtcg
     1681 ctcggcatcg cgctcggtgt cggcttcctc ctcggcgtcg tgatgggccg ctagcgcctg
     1741 gtcgccttcc gggcgtcgct gcctaatggc tcatcagcac cgcgacgtgg gtgttgcgca
     1801 acacgtgaag cgtcgccccg ccgaagatcc attcccgcca gcgccagcgg ccataagccc
     1861 ccatgaccaa gaggtctgct ttgccggtcg cggccagtag cgcttcccca accgcgcggc
     1921 cttcgagact ggccctctcg atctccaccg gatagccggc ccgcgtcaga tagtcggcca
     1981 tgccggaggc ggtgccatcg ctctcctcgc cttcgtcgag gcaaagcagc ctgacgcgat
     2041 tggcggtggc caagaacggc gcagcggacg cgacggcacg aaccgcctcg accgtatcct
     2101 tccaggcgat aacgacagtc tcgcccagcg gtcccgccgc cttatccggg acaacgagaa
     2161 tgggacggcc cgactggaac agcgcggcgt cgcgcgcggc gctccaaagc tcgtcctcct
     2221 cgtccgcgac gctgggtagc acggtcacgt cggcgatctt ggcccgcatg gcgacggccg
     2281 ggccgacgag cccttcgacc gtgacgagtt cggcggcaac ctccggatgg gccccgagtt
     2341 gttcctcgaa ccagtccgtg gcttttcttt cctcgtagtc gacctgtttc ctggaagcgt
     2401 cgaccatttc ctcgaaataa acggggctga gctcggtcgc ataaggcagg cgctggaccg
     2461 gcggtgggca gggatgcagc acttcaaggc ctgcgccgaa gcgctccgcg aggcgaaatg
     2521 ctgcctcagt gcaggctcct gaggcgccac cggcaccgat agggaggaga atcctgacaa
     2581 gcttcacggg agcgtccctt tccttagctt cgcggcgtct tcggcccgat ccaatgacat
     2641 gccaaaccgc catgccttga ggcgggtcaa gacccgttcc tggcttcttg acccatcgca
     2701 aagaagtgtg tcgccttttc tccttattct agcgcacaat ggtccaagca aatcgatagc
     2761 cggcaacaaa agataaagag gcgatcatgc aactccccct cgacatcagc ttccgcaaca
     2821 tggacccctc gcccgccgtg gaagcgcgca tccgcgagaa ggcggcgaag cttgagcgtt
     2881 ttcacgaacg cattatcagc tgcaccgtcg tggtcgaagc gccgcaccgg catcatcaca
     2941 agggaaagat atacagcgtg cacatcgaca tcagcgtgcc cggcaaggac cacctgatcg
     3001 tcgatcgcgc caagccggcc gaccacgccc atgaagatgt ctatgtagcc gtgcgcgatg
     3061 ccttcaacgc ggcggtgcgc caactcgaag attacacgcg gcggatgcgc ggcaacgtca
     3121 aagcccacac ggcctcacgg ggggagccgt ccgccgccga atgatcggcg ccgttcaggc
     3181 cttccagaac gccttggctt tggcgaaacg cccataggcg cgcttcgcct ctttcaacca
     3241 cttctcctcc tcggcgatat cgatcgccgg tgcacgcaga cgccagtcct cttgaccagt
     3301 ggcaagcaaa gactttcggg tcgccatatc gttaagcacg ccgagcgcat cctggatggc
     3361 ctcaagcgcc gctaaggaca cgttccggcg ctcctcgctc ttcttcttgg ggaaggtcgc
     3421 ggcgaaaaac tccgtggcgt agcgcagtcg cttggaccgg atccgaagcc tgtgccgctt
     3481 ggccggcgac aggtctcgca agcgacggcc tttggccttg atcccacggc gcaagcgcgc
     3541 cagctgcttc agagcgtggc ggcggaccgg cttcttggcc agcttgcggt ttttgcgcgc
     3601 ccaatcgccc gtctcgaccc atgccgtgat atcgagcagt gcaacccgaa agcgatcgga
     3661 atttgcggac tgggttgcgc gggcgtaagc ggcggcgtga cgttccttga agtccctgta
     3721 ggtgtcctcc acctcacgat cgccgggata ggcggcacgc atcggctcca acacgtcggc
     3781 ggcgaacaca tcgagatcgc gcgcaggccc gagttcttgc gtgatccacc gcaacttgtc
     3841 cttaatggtc ttgcgctcgt cgccgccgag catctgccca aacagcgcga tcgcgacccg
     3901 caaacgcctt agcccgacgc gcatctgatg cagcgcttcg gcgcgcccct cgcgcatggc
     3961 gggcgcattg gccacgatct gccgcagaca attacgcgcg atggagcgga acgcctccgc
     4021 gcttgtcatg tcgggcgtga cgacgacacg ccgcgcgttc tcgaccgtat ggtctgcgtc
     4081 gtccagcaac tcaaagccac gttccgcctt cgtcttcacg gcaaggttca agggagcggt
     4141 ttcggcgagc tcgcgggcga gagagaacag agcggccttg gcgccggcct ttagctccaa
     4201 ttccaattcg gaaatcggcg cggccttgac gccggccgtt gcctctcctt ggtccacggc
     4261 caattcgatc tccgccccgc gatgccggac gtcataggtt ttgcgccgga acctggtgtg
     4321 gaagcgcggc ttgagatcgg cgcggacttc gggcgtgagc agcctggcga gcgcagtgcc
     4381 ctccgcggca tcgaggttgg gctcatgggt gggaaccgga cactcccatt cgttccgctc
     4441 taagaactcg ccttcgctct tggcgctctt gatggtttgt acgtagccgt cacccgtgga
     4501 acgcacgcgc aagaagacgc ccgcattgcg cagatcactg gcgccggtgt cgtagtagat
     4561 ggagacgagt tcgcgcgttt tcggcggcgg cccctcgaaa atcggatgcg acagaatgcg
     4621 cggcgcagcg tcggcgtcga actcgagctt gagttcgatc tccttcgcgt tagaacttga
     4681 ctgagactca caagatgcca cggccgcaaa ctcctgatct gcacggttgg cgggcgggtt
     4741 cgcgtcgtcc gcgcgcgcac tattcgcccc gagaaagcag ggccagaaga gaagcctaca
     4801 aagggattat cacaatcaac acccgaatgt cccgcaaccc ccgacgtccc gtgcaccaat
     4861 gatgcgtgcc ttgggcgcct gtcgcaaatt tccagcggac aaaaggttat tgtgcctcag
     4921 accgtctgga gcggccgccg cggcacaggg cgcttgcgcg ctctggtggg cgagcttctc
     4981 acggtcgtcc cgggaaaaac gttctcgtcc gactccagaa tcgcgacacg gtcacgcccc
     5041 gctcgcttgg cgacgtacag cgcggcatcc gcacgccgga atagtcccga gagatccgcg
     5101 tccacgtccg tgtccgaggc aacgccgacg ctcacggtgg cgccgactgc caaaccgttg
     5161 acgaaggcgc ccgcattgga gaaggcgcgg cgcacgcgct cggcggcggc aaccgccctc
     5221 ttggcatcga tgccggggag gatggccgcg aactcctcgc cgccaagacg cccgacgatg
     5281 gcgttctcct tgagatcccg ctccgcgatc atggcgaaaa tcttgaggac ttcgtcgccg
     5341 aaactatggc cataggagtc gttggtgtcc ttgaaatgat ccagatcgaa gatcagcacc
     5401 gacaccggcg tgccacccag aacacggcgt tctccgacgg tatcggcgac ttcgaagagc
     5461 gcgcggcggt tcggcagccc ggtcagactg tcggtgagcg catcgcggcg ctgctcctgc
     5521 tcctcgcgct ccttggccat ggacagcaca atgaacgcca aggcgacacg catcagcatc
     5581 gtcacaagcg caaccgccgg aagccaggca atggtgaagg cctcacccag agtctggatc
     5641 gggacgacga gaatgaaggg gatccaggac aggtagctca gccccacaag cacaagaagg
     5701 ctgacggcgg gccagcagag ctgcctgtcg ccaatccggc gcagctccag cgcggcggca
     5761 aaattatacg ctgcaaagaa gatgcacccg acaatcaggc gcgcatctag ggattcgacg
     5821 aaccccgaat gggacaggac aatgaatccg acgggaccga acatgatcca aaacggcgcc
     5881 cgctcgtgtt cggcatactc gcgggccgca tcccacttca acgcgacggc caagatggcg
     5941 atcgacgcac cgatggtgac caggttcacc tcgttcgcga gcgcggcgcc cagcacgatg
     6001 gcggcgcccg cgacatgggt cagcatggcc agaccccagg cgccgacgaa gcgcgggccc
     6061 cccgcctgct cccaaccggc tcggccccag gtcagcaaca gcatggcagc gacaatggcc
     6121 gtgaccgaaa ggttcacaac cgacatcgtc atcgtgtcga gatgcatgcg ccgcccctcc
     6181 catggaaatc caccggggcg cgaccctaaa gatagggtct cacgacacgt tttattttaa
     6241 cgcgagactt tctggaaaat tttagcgaac ggctttcgtg tgggggcctc agtcgccgcg
     6301 cttcaccgct tcgaagaggc cggcattggg gcagaacggc tgcggctgct tttcctcagg
     6361 ctcgttcttg agctcgctcc tggcctccgt gagccaatcc cggcagccgt ctttacatat
     6421 gcaagcatgg caattcgacc gtgcccgcaa ataggactgg ccgccatcca cgtcgatgac
     6481 gtcgagcaga tcgacgccga cgacctgcat catgtcgtcc atgagtactt ggtgatgaaa
     6541 gccggcgaga tcttgccttg ggcgcttcat ggtgtcccct cccccacagg gcgcgcagct
     6601 tcgcgcatta ccttggctaa cggacgggag gggtctctgc cttgaccaag atcaagcccg
     6661 ctaagcgttg gtgccagccc gctccatgcc ggtgcggatc agctcggcgg cctcaccgac
     6721 ttcggcccag cgcatgacct tgacccattt gcctttctcc aagtctttgt agtgctcgaa
     6781 gaagtgcccg atctgctcga ccagaatttc gggcaggtcg cgatagctcg tcacgcgctt
     6841 gtagaacggg tgcagctcgt cgatgggcac ggcaatgatc ttctcgtcga tgcccgcctc
     6901 gtcctccatg aggagcgcac caacagggcg ggcgcggatc accgtgccgg aagcgacggg
     6961 cgtgggggcg atcacgatgc agtccacagg atcgccgtcc tccgacagcg tgtgcggaat
     7021 gaagccgtaa ttggccggat acatcatgga ggtgtgcagg aagcggtcaa cgaagagcgc
     7081 gcccgacttc ttgtcgacct cgtacttcac cggcgtcccg ccttgcggaa tctcgatgat
     7141 gacgttgact tcatgtggcg gattatctcc gaccgggatc tgggaaaggt tcatagctcc
     7201 gatagggtct ccatgttggc gccggcccgt tcgtcgagcg cgaccgtctt gatcaacgcg
     7261 aaaatgcggc cgcccgcgcg gatcgcaagc tcgtcgaccg ctttgcgggt ggcgagggcg
     7321 aaaagatgcc catggccgtc gagcgcgatc gaaacgccgg cgaaggcgcc accgtgggtg
     7381 tcgacctcct cgacggttcc tgccagcacg ttgcgaatgc ttaagttcgg tccggcgtcc
     7441 cgcgccagcg taatgtccgt cgccttgacg gcaacgcgga cctctcgccc tttgggcccg
     7501 gcgttgccca cgagccagat cttgcccgcc ggatgcgcga tctccgtgag cccatagtcc
     7561 ccgtccacgg cggccacagc gcccctcacc acggaggaac gcgcgagggg gctgataccg
     7621 atgccgcgca aggcggggcc aagcgtctcc tcgacgggac ctgcggcgac gacgcggccc
     7681 gcgtccaaca cgacaacacg cgaggcaagg cgcgcgacct cgtccaccgc gtgcgacacg
     7741 tagacgatcg gaatgccgaa cgtgtcgcgc aagctctcga tcagcggcag aatctctagc
     7801 ttgcgttcgg tgtcgagcga ggccagcggc tcgtccatga gaagaagcgc gggcgaggcc
     7861 agcagcgcgc gcccgattga gacgcgctgc ttctcgccgc cggacaggct cgcgggacgc
     7921 cggcgcagca aatgcccaat gcccagcgtc tccaccacct tgtccaactg cacatgttcc
     7981 gggaccttgc cggcgaactt ccgcccgtag aggaggttct gcttgatgct gagatgggga
     8041 aacagacggg cgtcctgaaa cacgagcccc atctgcctgc ggtgcggggg cacgaaggtg
     8101 ccggcctcaa tatccaccaa ggtgcggccg tcgagcacga tgcggccgcg atcgggccgg
     8161 gcaagacccg cgatcaggct gatggtcaga gactttccgg cccccgagcg gccgaacaga
     8221 caggtgatac cggcctcgtt ctcgaaggcg gcatcgagat cgaagtcatc gagcttgagc
     8281 gccacatcga cgctgatcat tggttggcct cggctatcat ggctccgctt agcccgaacc
     8341 cgtccttgcg gtctaggcga ctttagcatg ggccacgggc ccatacggga ctaggcacgc
     8401 cgggggcgct tcgggtaaaa ccggcaccag atcgactgtc actcaagagg acattttatg
     8461 cgcccaacat tccttgcgtt cttgctgaca ctgtccgccc tggcagccac cggccccgcc
     8521 accgccgagc cggacatgga taccgtgcgc tgtacggcta ttcgggatgc cgctcaatgc
     8581 gtatcccgcc ccgattgctg gtacgacgca gctggcggca acggctgctt gaagggccca
     8641 cgccccgacg aaaatccctg tcaggtgcac ggcagcaagt ccgtctgcaa tacgagcacg
     8701 cttggctgca cctggaacga ggccgagagc aagtgcgtta gaaaggcaaa ctaggccgcc
     8761 gcgacagaag gaagccatga tcgagctgct gaaaggtttt cccgacaatg tggtggccct
     8821 ggcttgccat gggcatgtga cgcgggagga ctacgacaag gtcctggttc cggcgttgga
     8881 gaaagcgctc cggtcgaacg acaaggtgcg cgtactctac gagatcggtg gcgacttcga
     8941 aggctacgac gtcagtgctt cttgggagga cttcaaggtc ggcatggagc acctaccccg
     9001 ctgggagcgg attgcggtca tcagcgacat cgaatggatc aagcatacga tgcagctctt
     9061 cagcttcgtc atgccgggca cagtccgggt ctatccgacc gaggaggccg cgaaggcgcg
     9121 gctgtggatc acgcaggccg cgtgagcccc tgccaatc
//
LOCUS       DBNHVU010000154        17666 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1318389, whole genome shotgun sequence.
ACCESSION   DBNHVU010000154 DBNHVU010000000
VERSION     DBNHVU010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 17666)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17666)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17666
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1318389"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="AC16Y7_06205"
     CDS             complement(<1..1075)
                     /locus_tag="AC16Y7_06205"
                     /inference="COORDINATES: protein motif:HMM:NF013871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0052929 - ATP:3'-cytidine-cytidine-tRNA
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIR5709008.1"
                     /translation="MAEPSSSRAVAPPSLKDAAWLQRPETRCVFAALTGDGIETRAVG
                     GAVRNTLLGRDVSEVDLATTALPKTVMTMARKAGLKAVPTGIAHGTVTVVVDGESFEV
                     TTLRRDVETFGRHATIAFTEDWTEDAQRRDFTLNALYADADGTLYDPLGGYDDLIAGR
                     VRFIGEADERIKEDYLRILRYFRFNAYYGKDGLDPAGLAACVRQHAGLAKLSAERVAG
                     ELRRILIAPRAFAAVKALFDYGLLSGVLSAAPRLDRLSRLIAIEEGLSRAPDPALRLA
                     ALSVFVREDAPRLAARFKLSNAEQAVLALGADDGLDGELPDEDTAKRLLYRLGPDRFA
                     LQVLLAWAGSDAAPDDSDWREALT"
     gene            complement(1090..1350)
                     /locus_tag="AC16Y7_06210"
     CDS             complement(1090..1350)
                     /locus_tag="AC16Y7_06210"
                     /inference="COORDINATES: protein motif:HMM:NF040327.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6111 family protein"
                     /protein_id="HIR5709009.1"
                     /translation="MLRIALIELLIFAVPFLLYAVYVIWVKGAPPSAVWQGAPLLWLF
                     AAAVGLLLVAMLVLVQFSGGDREGTYHPPVLEDGVIRPGSVD"
     gene            complement(1423..1899)
                     /locus_tag="AC16Y7_06215"
     CDS             complement(1423..1899)
                     /locus_tag="AC16Y7_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006937681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HIR5709010.1"
                     /translation="MPNLNICGDIGLRIARDGTWYYQGSPIGRKPLVKLFASVLRCEE
                     DGFYLVTPVEKVPIAVEGEPFIAVEMTAAGEGPSQRLSFRTNVDDEATAGPGHEIGFR
                     PEPGGGHAPYVVVREGLRAQLARPVYYELTELAVETEQGTGVWSGGAFFAFPMDGD"
     gene            2123..3118
                     /locus_tag="AC16Y7_06220"
     CDS             2123..3118
                     /locus_tag="AC16Y7_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5709011.1"
                     /translation="MTTLESDTQLVDRLDEIGAKIQDVRETTGTVIFGQERVIDLALI
                     TLLSGGHALLIGVPGVAKTSLVETLGAVLGLDNKRIQFTPDLMPSDIIGSEVLEEEAG
                     GRRSFRFVKGPIFTQLLMADEINRASPKTQSALLQAMQENHVTTAGVRHDVPRPFHVL
                     ATQNPLEQEGTYPLPEAQLDRFLLQIDMSYPDADAERRMLFATTGVEERELKTSLSAD
                     ELMLAQRLVRQLPVGDQVVDAILQLVRSARPGTGVDDTMDEMIAWGPGPRASQALMLA
                     VRAKAMMEGRLAPSVDDVVDLAEPVLKHRMALTFAARAEGVDMSDMMTRLIEPLR"
     gene            3126..4073
                     /locus_tag="AC16Y7_06225"
     CDS             3126..4073
                     /locus_tag="AC16Y7_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR5709012.1"
                     /translation="MANAKATSLQPFLAEEAEAHGLAARMPALLIEAQRVAHTVTHGT
                     HGRRRPGPGETFWQFRHFDTNDSVAGIDWRRSASSDHLFVREREWEAAHTVWLWTDLS
                     PSMRFQSSLSNTSKESRAVVLTLALTELLSRAGERVGLFGGRPFAGRNTSRKVAEVLL
                     NETAEGSLPPDARLSRFSECLLFSDFLEPVDETVAKLQAIASLGVRGHLVQILDPAEE
                     TLPYAGRTEFAASEGGDRLIAGRAENLRDSYLKRMKRHRIDLQEAARRLGWSFVVHHT
                     DRPPEEVVLAVHNHLAGLDRDYRFRAAGGKTGHTEAGAA"
     gene            4070..6922
                     /locus_tag="AC16Y7_06230"
     CDS             4070..6922
                     /locus_tag="AC16Y7_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HIR5709013.1"
                     /translation="MMTLGSIGFLQPWLLVALAALPAIWWLLRFTPPSPKVVVFPPTR
                     LLKDLKPTEETPARSPWWLTLLRMLLAALLILALARPVLNPDRETFAGEGPLLLVVDN
                     GWASAAHWGGRREAIAAAIDRAGRDGRMVILAPTATAQPLGEPLPADELRERIAGLIP
                     EPYAPDRMGLAEILEKDIKPASGYSVLWLSDGLDYGKSEDFAGRLAKLAGDGGSLSVL
                     KPDGSETPLAVGRASGEGGVLAGRVLSGADGPRTGTVSALTGRDEPLGEAPFKIKEGE
                     REAQAEFELPLEIRNQVARIQIKGERSAGAVHLLDARSQWHRVGIVSGESREEAQPLL
                     SPLYYVERALSPYADVVRPNETNVATAVHDLIDKQRVSTIVLADIGKLVAGTQEELEA
                     WLKDGGVLIRFAGPRLEQGGDELLPVALRRGGRSLGGSLSWSTPQPLAPFEDKSPFKG
                     LEIADDVRVNRQVLADPTVAMDAEVWAALADGTPLVTAAKRDEGWIVLFHVTANSDWS
                     NLPLSGLFVEMLRRAVALGPSQVGAATGDEAASVEAGTPTPAATTAFAPIQTLDGFGQ
                     LVPPPLRAAPISPDAFDKTTPSAEHPPGYYGPSSAAHALNVVNAKTALTPLTSVEGAS
                     TVSGYTLKKPFALEPWLYIAALALFAIDILAVLALSTGFKFRRRGAVAAAIILAFALV
                     PLAFTAPSFAAEIKSAESTGNADDDFALRASLKTRLAYVMTGDADIDRTSQEGLGGLS
                     KVLQARTALEPGAPMGVDIDKDELSFFPVLYWPVREDAEPMSDKTLAKIDAYMKQGGM
                     IIFDTRDQERVSYGGVQGKALNRLIGQLDIPPLEPVPENHVLTKSFYLMHSFPGRWDG
                     GSLWVEAAPAGEAERSERSRRTDGVSSLVITSNDLASAWALDEANRPMFPVVPGGEVQ
                     REMAFRAGVNLVMYALTGNYKADQVHVPALLERLGQ"
     gene            6926..9022
                     /locus_tag="AC16Y7_06235"
     CDS             6926..9022
                     /locus_tag="AC16Y7_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709014.1"
                     /translation="MSWSIEFIPFVPWPVLWVIAAIGAGLLALLFWRARRGAILRLLA
                     FALLMLALANPHLKQEDREALNDIVTVVVDDSQSQAIAGRTVRTEEVREALEERLKEI
                     PDLDVRFVRSNSVSADNERDGTLLFTDLGQALADVPPDRLAGVVMITDGEVHDVPGSA
                     SALGFDAPVHTLLTGKQGEFDRRLEILTAPRFGIVGSVQPIEVKVSETKPGETNIVTL
                     TVTHQGRPPSKEVVRIGDTVEIPVEIDHAGPNIVEIETESAPGELTTINNRAVLTVEG
                     VRENLRVLLVSGEPHAGERTWRNMLKSDASVDLVHFTILRPPEKQDGTPINQLSLIAF
                     PTRELFQEKLDQFDLIIFDRYQRRGVLPLLYLENVSRYVERGGAVLVSAGDDYASALS
                     LYRTPLGQILPAVPSGRVLDEPYKPALTDLGGKHPVTSELPGARGGGPNADEPTWGRW
                     FRVVDTSPREAEVLMKGAGDRPLLVIGERGKGRVALLLSDHTWLWARGYDGGGPYSHL
                     LRRLAHWLMKEPELEEETLKATSKGQTLIIERRSIEDEIDPVTITTPSGKQEAVTLDK
                     GERGIWRKTMTVDEQGVYRVASGELASIATVGNANTRELSAVTATDAPLSPILEETGG
                     GAFWLGRKDGEASSLPRLAMVRSGHVMHGTDWLGLHKRDAYHVKGVRLFPLFTGFLAL
                     ALLLGLLAATWYREGH"
     gene            complement(9023..9526)
                     /locus_tag="AC16Y7_06240"
     CDS             complement(9023..9526)
                     /locus_tag="AC16Y7_06240"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006940054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5709015.1"
                     /translation="MTTFEILSQTAADEAAMSTLSAVAFGPGRFTRTAYRVREGVPPV
                     SALSLAGWLDGRLVGGIHFTAITIGDSEGGLLLGPLVVDPDVKGSGYGKALVGEGLAR
                     AKDQGFGLVLLVGDMPYYERFGFVPVVRGKIALPGPVDPARLLAAELVPGALDAAAGQ
                     VKAQADH"
     gene            9890..10510
                     /gene="wrbA"
                     /locus_tag="AC16Y7_06245"
     CDS             9890..10510
                     /gene="wrbA"
                     /locus_tag="AC16Y7_06245"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HIR5709016.1"
                     /translation="MTDTAQLLVLYHSSYGHTETLAYAMAHGVRAVEDIQVTVKRVPE
                     LMPEDVMRGAGMKVEQPAEIADPQELANYDGIIVGTPTRFGNMSSQLRYFFDQTGPLW
                     VAGALIGKVGSVFTSTSTGGGNETTIVSVHSTMLHHGMVVVGLPYSAPDLPDISEVKG
                     GSPYGAGTIAAPDGSRVPSQKELNLASFQGHHVAKITKRLAGGLDD"
     gene            complement(10507..10998)
                     /locus_tag="AC16Y7_06250"
     CDS             complement(10507..10998)
                     /locus_tag="AC16Y7_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR5709017.1"
                     /translation="MSSQRPTGPALSRLVTATLRPFWRLRRGMTLGAQGVVIDEADRL
                     LLVRHSYRPGWFFPGGGVEWGETLEEGLARELGEEVGVTLTGPPLLHGIFSNNANFPG
                     DHIAVYLIRAWTRRGRHVQLGEIAEAEMFAADDVPAQTDAGTRQRLAEIFGGASVSPK
                     WSA"
     gene            11120..12031
                     /locus_tag="AC16Y7_06255"
     CDS             11120..12031
                     /locus_tag="AC16Y7_06255"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR5709018.1"
                     /translation="MFTLAHLSDVHLSPLPRVKRTDLLSKRLLGYVNWHRGRKFVHRR
                     EYLDLLTRDLAERDPDHIAVTGDLINLGLPKEYLRAADWLHHLGNPNDVTAIPGNHDA
                     YVRMHPDTSTRHWQPYMTSNEAGEALFTTPTGGFPFVRCFGDIGIVALSSAIPTMPFI
                     AAGRLGKAQLADLANALDAMGREGLFRTVLIHHPPLPGQAGWRRGLRDADALRDVLEA
                     HGADLVLHGHNHAQTVHELQAASGPTIVVGVPSASDAVDGRTPAARYNEYNIERAGNG
                     WRVEMIGRAAADLDQVWECERRVLRER"
     gene            complement(12018..12581)
                     /locus_tag="AC16Y7_06260"
     CDS             complement(12018..12581)
                     /locus_tag="AC16Y7_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709019.1"
                     /translation="MDDNVKMIARPAASRSLREAIGKARLEEAERLDATADTRDGEIA
                     RLELLKAELETVFAEIPKSDDRFSLVLAPSRPARLWIDLFTYVAVDDGSGAYLFIRNS
                     ENGRRTLFSANTVAEMADRITKYMAQEIVRRERMEAALVEAPPRQKTVIEPESSGPGM
                     GIVLSAFIIGVITGAAGLFASVWLSVP"
     gene            complement(12819..14159)
                     /locus_tag="AC16Y7_06265"
     CDS             complement(12819..14159)
                     /locus_tag="AC16Y7_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709020.1"
                     /translation="MPRQGTALTTTSGSEVTEHHGAEETPVKATRRSRAVKQKVVKPS
                     AFGMFLKPIGMAIGFVLIAYAILSLGTTFYRSYTRHADHTAPVHPDASIFDIFMPSRT
                     LPPDAVDLVVKDVDGKLHRVLVSKSETDEFVNDTILMLDDERARIKEAARNDIDRTFA
                     LAFQDRDETIEAYADWFFEWKRSYIVLKETISSAISRVLETGKYESLGEAIEADVKEY
                     FLESYTEQVLKPEIRDQTITKGVEQAVRHAHDSYRRVIANGDMRLQLFLAQHTSHMED
                     IPASSSMTNVKLDWDAQKWKAPHYLMEDRAFDGVVGVGAAAAGGTVGALTLGRAINAI
                     VSRSFGQMSARVATSLATRAAPALQGAAVGTAVQPVGGQVIGAAIGVAIGIAVDYLAN
                     EASEAVNRDSFVAANEEALEATIVAWRSGLEASVDTAIDKWFDDARASVVLAAK"
     gene            complement(14300..15025)
                     /gene="otsB"
                     /locus_tag="AC16Y7_06270"
     CDS             complement(14300..15025)
                     /gene="otsB"
                     /locus_tag="AC16Y7_06270"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HIR5709021.1"
                     /translation="MSQPLPPLGESSAFFFDFDGTLAALAPRPELVEVEPRVGDLLRG
                     LCGRFDGAVAIVTGRPLDVVDGFLAPLRLATAAEHGSIRRDASGHVYADTRSLQAVIA
                     VGDALTGFVEDNAGLLLERKQTSVALHYRQRPELKETCRTAVEEAVADNDGLVILPGK
                     MVYEVRPKGCDKGVAVTAFLDEPPFKGRLPVYMGDDVTDEDAFAVVNALGGASIKIDD
                     GESQAQYRTDREALFDWLAELVH"
     gene            15290..16711
                     /locus_tag="AC16Y7_06275"
     CDS             15290..16711
                     /locus_tag="AC16Y7_06275"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
                     /protein_id="HIR5709022.1"
                     /translation="MTGKTRRLVVVSNRVADFNAKFQTGGLAVALGDALKASKGVWFG
                     WSGDVSDDALAEAPKVENIEGLTVATIDLTPQEAEGYYYGYANRCLWPALHYRLDLAQ
                     SNEEHSEVYFDVNARFADALMPLIEPDDLIWVHDYHLIPLAEELRGRGCTSRIGFFLH
                     TPFPSPEIFAAVPGQKRLGNALMVYDVVGFQTTSDRDNFRRYVAASLDGEQLHGDRIA
                     AQGETTLASVFPIGIDAAAFRKLAESESTKDAILPDLGEDMKTIIGVDRLDYTKGLPE
                     RLRAFETLLEEFPEHHGKVRLVQIAPPTRETLPVYQGMREEVERLVGRINGRFGDLRW
                     TPVTYLHHGVPQDILTRLSRESAVGLVTPLRDGMNLVAKEYIAAQAPQDPGVLVLSRF
                     AGAAEQLREALLINPHDVNELARALDRALTMPRQERWARHNRLWQRIETYDLANWRDS
                     FIQTFAESDTDPVNLFAKKAGAA"
     gene            16883..>17666
                     /locus_tag="AC16Y7_06280"
     CDS             16883..>17666
                     /locus_tag="AC16Y7_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR5709023.1"
                     /translation="MIDRTDRLWYKNAVIYELHIKAFFDSDNDGIGDFAGLMRKLDYL
                     QELGVTAVWLLPFYPSPLRDDGYDIADYTSINPTYGTMRDFRQFVAAAHDRGIRVITE
                     LVVNHTSDQHPWFQRARKAKPGSVWRDYYVWSDTDQKYPGTRIIFLDTETSNWTWDPE
                     AKAYFWHRFYSHQPDLNYDNPRVLADILRVLRFWLDMGVDGLRLDAVPYLVEREGTNN
                     ENLPETHELLKKFRAELDQRYPDRMLLAEANQWPEDTRPYFGD"
BASE COUNT         3241 a         5972 c         5384 g         3069 t
ORIGIN      
        1 aggtcaatgc ttcgcgccaa tctgaatcgt caggcgccgc atcgctgccg gcccaagcaa
       61 gcaaaacctg caaggcaaat cggtcgggcc cgagccggta gagcagccgc ttggcggtat
      121 cttcgtccgg cagctcacca tcgagcccgt cgtcggcgcc gagcgccagc acggcctgct
      181 ccgcattgga tagtttaaag cgcgcggcga gccgtggcgc atcttcgcgc acgaacaccg
      241 aaagcgcggc aagtcgaagc gctggatcgg gcgcgcgcga caaaccctct tcaatcgcga
      301 tcaaccgcga tagacggtca agccgcggcg ctgcgctcaa gaccccgctc agcaggccat
      361 aatcgaacaa ggcctttacc gcagcaaaag cccgaggcgc gatcagaata cgccgcaact
      421 cgcccgccac gcgctctgcg gacagcttgg caagtccggc atgctggcgc acgcacgcgg
      481 caagtccagc cgggtcaagc ccatccttgc cgtaataggc attgaagcgg aagtagcgca
      541 ggattcgtaa gtagtcttct ttgatgcgtt cgtccgcctc gccaatgaaa cgcacgcgcc
      601 ccgcaatcaa atcgtcatag ccgccaagcg gatcgtagag cgttccgtcg gcatcggcat
      661 agagcgcgtt cagcgtgaag tcgcgccgct gcgcatcttc ggtccagtcc tccgtgaagg
      721 cgatcgtggc atggcggcca aaagtttcca cgtcgcgccg cagcgtcgtg acctcgaagg
      781 actcgccgtc caccacaacc gtcaccgtgc catgcgcgat cccggtgggc acagctttga
      841 gaccggcctt gcgcgccatc gtcatgaccg ttttcggcag cgccgtggtg gcgaggtcga
      901 cttccgagac gtcacgcccc aagagtgtgt tgcgcaccgc gccgcccacc gcgcgcgttt
      961 cgataccgtc gcccgtgagc gccgcgaaca cgcaacgcgt ctccggccgc tggagccatg
     1021 cagcatcctt cagcgaaggc ggcgccacgg cgcgggaaga agagggctcg gccatgaagc
     1081 ctctaaaggt cagtcgacac tgccgggcct gatcacgcca tcttcgagga ccggcgggtg
     1141 ataggtgccc tcacggtcgc cgccggagaa ctgcaccagc accaacatcg cgacgagcag
     1201 caacccgacg gccgccgcga acagccacag gagcggtgcg ccctgccaca cggccgacgg
     1261 cggcgcgcct ttgacccaga tcacgtagac cgcgtagagc aggaagggca ctgcaaaaat
     1321 cagaagttcg atgagggcga tgcgcaacat cggctggatg gtctcctggc gtttcgattg
     1381 tcgggaacct tagctttgca cgccgcgcaa tgcgagtggg cttcagtccc cgtccatagg
     1441 gaaggcaaag aaggcaccgc cgctccacac gcccgtgccc tgctccgtct ccaccgcgag
     1501 ctccgtgagc tcatagtaga cgggacgcgc cagctgcgcc cgcaaaccct cccgcaccac
     1561 gacatagggc gcatggccgc cgccgggctc gggccgaaag ccgatttcgt gccccggccc
     1621 cgcggtcgcc tcgtcgtcca cattggtccg gaaggagaga cgctggctgg gcccctctcc
     1681 cgcggccgtc atctcaaccg caataaaggg ctcgccctcg accgcgatgg gcaccttctc
     1741 cacgggcgtc acgagataga agccatcctc ttcgcagcgc aacacggagg cgaataactt
     1801 gaccagcggc ttccggccga tggggctccc ctggtaatac caagtcccgt cgcgggctat
     1861 tctcaggccg atatccccgc aaatattaag gtttggcaat aatggcttgc gtggtttgcc
     1921 gccgtcttgg gcagatgtta cgagtctttc tctttcaact tggtcgggca tcaccatatc
     1981 cgcctgtagg ccatcgtctt tctcttccga agcgccgctg cagcaaacgg tattgtgacc
     2041 caattaccga tcaaagtgtt ccgcttttcc aggcctttgg ccctatattt tcccatcatc
     2101 aggatcgagc gaaactggag ccatgacgac acttgaatcc gatacacaac tggtcgaccg
     2161 cctggacgaa attggcgcga agattcagga cgtaagggag acgaccggga cggtcatctt
     2221 cggtcaggag cgcgtcatcg acctcgctct gatcacgctg ctgtcgggcg gtcacgccct
     2281 gctgatcggc gtgccgggcg tcgccaagac gagcctcgtg gaaacgctgg gggccgtgct
     2341 cggcctcgac aacaagcgca ttcagttcac gccggacctt atgccgtccg atatcatcgg
     2401 ctccgaggtg ctggaggaag aggccggcgg ccgacgcagc ttccgcttcg tcaaggggcc
     2461 catcttcacc cagctcttga tggccgacga gatcaaccgc gccagcccca agacccagtc
     2521 ggccctgctt caagccatgc aggagaacca tgtcaccacg gcgggcgtcc gccacgacgt
     2581 gccgcggcct ttccatgtgc tcgccaccca gaacccgctg gagcaggaag gcacctatcc
     2641 cctgcccgag gcgcagctcg accgcttctt gctgcaaatc gacatgtcct atccggatgc
     2701 agacgccgag cggcgcatgt tgttcgccac cactggcgtc gaggagcgcg agttgaagac
     2761 gagcctctcc gccgatgagc tgatgctggc tcaacggctc gtgcgccagc tgcccgtcgg
     2821 cgatcaggtg gtggacgcga tcttgcagct cgtccgctcc gcccgtcccg gcaccggggt
     2881 cgacgatacg atggacgaga tgattgcctg gggtcccggg ccgcgcgcca gccaagcgct
     2941 gatgctggcc gttcgcgcca aagcgatgat ggagggccgg cttgcgccgt ccgtcgatga
     3001 cgtcgttgat ttggccgagc ctgtcttgaa acaccgtatg gcgctcacct tcgccgcgcg
     3061 cgccgaaggc gtcgatatga gcgacatgat gacaaggctc attgagccgc tgaggtagcc
     3121 aacccttggc aaacgcgaaa gcgacatcac ttcagccgtt tctggcggag gaagccgagg
     3181 cccatgggtt ggcggcgcgc atgcctgcgc ttctcatcga ggcgcaacgc gtcgcgcaca
     3241 cagtgacgca cggcacccac ggtcgacggc gtccgggccc cggcgaaacg ttttggcagt
     3301 tccgtcactt cgacacgaat gactcggtcg cgggcatcga ttggcggcgg tcagcgagct
     3361 ccgaccatct gttcgtacgc gagcgggaat gggaagcggc gcacacggtc tggctttgga
     3421 ccgacctgtc gccctccatg cggtttcagt cgtccttgtc gaacacgtcc aaggaaagcc
     3481 gcgccgtcgt gcttacactt gcgctcaccg agcttctgtc gcgcgccggc gagcgcgtgg
     3541 gactgttcgg cggccggccc ttcgcaggcc gcaacacaag ccggaaggtc gccgaagttc
     3601 tgttgaacga aacggcggaa ggaagcctcc cgcccgatgc ccggctcagc cgcttttccg
     3661 agtgcctcct gttctccgac ttccttgagc cggtggacga gacggtggcc aagcttcaag
     3721 caatcgccag cctgggcgtg cgagggcatc tcgttcagat tctcgatccg gccgaggaga
     3781 cactgcctta cgctggccgc accgagttcg ccgcgagcga aggcggtgac cggctgatcg
     3841 ccggacgcgc ggagaatttg cgcgacagct acctcaaacg gatgaaacgc caccgcatcg
     3901 atcttcaaga agcggcgcgc cgcctcggct ggtcattcgt cgtgcatcac acagaccggc
     3961 cgccggaaga ggtcgtactc gccgtccata accatcttgc agggctcgac cgcgactacc
     4021 gctttcgcgc ggctggcggc aagacgggac acactgaggc gggcgccgca tgatgacgct
     4081 gggatccata ggcttccttc agccttggct gctggtggcg ctcgcggcac tgcccgccat
     4141 ttggtggctg ctgcgcttca cgccgcccag tccgaaggtc gtggtgtttc cccccacgcg
     4201 cctcttgaag gacttgaagc caaccgagga gacgccggcc cgcagcccct ggtggctgac
     4261 cctgctgcgc atgctgctcg ccgcacttct gatcctcgcg ctggcgcggc ccgtgctcaa
     4321 tcccgaccgc gaaaccttcg ccggcgaagg cccgctgctt ctcgtggtgg acaatggctg
     4381 ggcgtccgcc gcccattggg gcggacgccg cgaggcgata gctgccgcca tcgatcgtgc
     4441 cggccgcgac ggacgcatgg tcatcctagc gccgacggcc acggcacagc cgctcggcga
     4501 accgctcccg gccgatgagt tgcgcgagcg tattgctggg ctgatccccg agccatacgc
     4561 gcccgaccgc atgggccttg ccgaaatatt ggagaaggat attaaacctg cgagcggcta
     4621 cagcgtcctc tggctctccg acggactcga ttacggcaag agcgaagact ttgcaggccg
     4681 cttggccaag cttgccggcg acggcggcag cctctcggtg ctcaagccgg atggcagcga
     4741 aacgccgctc gcggtcggcc gtgcctccgg tgaaggcggc gtgctggccg ggcgtgtgct
     4801 gagcggtgcc gacggcccgc gcaccggcac ggtgagcgcg cttaccggac gtgacgagcc
     4861 cctcggtgag gcacctttca agatcaaaga gggcgagcgc gaagcccagg cggaattcga
     4921 gctgccgctg gaaatccgca accaagtcgc gcgcattcag atcaaaggcg agcgctccgc
     4981 tggtgcggtc cacttgcttg acgcccgctc gcaatggcat cgcgtcggca ttgtttccgg
     5041 cgaatcccgc gaggaggcgc agccgctcct gtcgccgctc tattatgtcg agcgcgcgct
     5101 ttcgccctac gccgacgttg tccgcccgaa tgagacgaac gtggcgaccg ccgtgcacga
     5161 tctcatcgac aagcagcggg tctccaccat tgttctggcc gatatcggca agctcgtcgc
     5221 cggcacccaa gaagaactcg aagcttggct caaggacggc ggcgtcctga tccggttcgc
     5281 agggcctagg ctcgaacagg gtggcgacga actcctgccc gtggcgctgc gccgcggcgg
     5341 ccggtcattg ggcggctcgc tctcctggag cacgccgcaa ccgctcgcgc cgttcgagga
     5401 caagagtccg ttcaagggtc tcgagattgc cgacgacgtg cgcgtcaacc gccaagtgct
     5461 cgccgacccg accgtcgcca tggacgccga agtgtgggcc gcgctcgccg acggcacacc
     5521 tttggtgacg gcggccaagc gcgatgaggg ctggatcgtt ctgttccatg tgaccgccaa
     5581 ttccgattgg tccaacctgc cgctctcagg cctcttcgtg gagatgctcc gccgcgccgt
     5641 cgcgcttggt ccaagtcagg tgggtgcggc gacaggcgac gaagccgcat ccgtcgaggc
     5701 aggcacgccc acgccggccg cgaccaccgc gttcgcgccc atccaaaccc tggacggctt
     5761 cggtcaactg gtcccgccgc ctttgcgcgc ggcgcccatc tcgccggacg ccttcgacaa
     5821 gaccacacca agcgctgagc acccgcccgg atattacggc ccgtcaagcg ccgcccatgc
     5881 gctgaacgtc gtgaatgcca agaccgcgct gacacccctg acgagtgtgg aaggcgcaag
     5941 taccgtgagc ggctacaccc tgaagaagcc cttcgcactt gaaccttggc tctatatcgc
     6001 cgcgcttgcg ctgttcgcca ttgacattct cgccgtcctt gctctgagca cgggcttcaa
     6061 gttccgtcgc cgtggcgccg tcgcagccgc tattatcctc gcattcgcgc tcgtaccgct
     6121 tgcgttcacc gcaccgagct ttgctgccga aatcaaaagt gcggagtcga cgggcaacgc
     6181 ggacgacgac ttcgccctga gagcctccct caaaacgcgt ctcgcctacg tgatgacggg
     6241 cgacgccgac atcgaccgca ccagccaaga gggccttggg ggtttgagca aggtgcttca
     6301 ggcccgcacc gcgcttgaac ctggcgctcc gatgggtgtc gacatcgaca aggacgaact
     6361 ctctttcttc ccggtgctgt attggcccgt gcgcgaagac gccgagccga tgtcggataa
     6421 gaccctggca aagatcgacg cctacatgaa acagggcggc atgatcatct tcgacacgcg
     6481 cgaccaagag cgggtgtcct atggcggcgt gcagggcaag gcgctcaacc ggctcatcgg
     6541 ccagctcgac attccgccgc tggagcccgt gccggagaac cacgttctca ccaagtcgtt
     6601 ctatctgatg catagcttcc ccggccgctg ggacggcggc tcgctttggg tcgaggccgc
     6661 gccggccggc gaggctgagc gcagtgaacg ctcccgccgt accgatggcg tcagctcact
     6721 cgtcatcaca tcgaacgacc tcgcgtccgc ttgggcactc gacgaagcga accggccaat
     6781 gttcccggtc gtacccggcg gcgaagtgca gcgggagatg gcgttccgtg ccggcgtgaa
     6841 cctcgtgatg tacgcgctca cgggcaacta caaagccgat caggtgcatg tccctgccct
     6901 actcgagcgc ttggggcagt aagccatgag ctggtcgatc gagttcatcc ccttcgtacc
     6961 ctggccggtc ctgtgggtca tcgccgcgat tggcgcaggt ctacttgcgc tgctgttttg
     7021 gcgcgcgcgc cgcggggcca tcttgcgtct cttggcgttt gcgctgctga tgctggccct
     7081 cgccaatccg catctcaagc aggaagaccg cgaagcgctc aacgacatcg tcaccgtcgt
     7141 cgtcgacgat agtcaaagcc aagctattgc gggccgcacc gtgcgcaccg aggaggtgcg
     7201 cgaggcgctg gaggaacggc tgaaagagat tcccgatctc gacgtgcgct ttgtccggtc
     7261 caactcggtt tccgccgaca atgagcgtga cggcacattg ctgttcacgg atttgggtca
     7321 ggcgctggcc gacgtgccgc cggatcgctt ggcgggtgtc gtcatgatca ctgacggcga
     7381 agtgcatgac gtcccgggct ctgcgtccgc gctcggtttc gatgcgcccg tccatacgct
     7441 gctcacgggc aagcagggcg agttcgaccg gcggctcgag atattgactg cgccgcgctt
     7501 cggcattgtc ggcagtgtcc agccgattga ggtcaaggtc agcgagacaa aacccggcga
     7561 gacgaatatc gtcacgctca ccgtcaccca tcaggggcga cccccgtcca aggaggtcgt
     7621 ccgcatcggc gatacggtcg agatccccgt cgagatcgac catgccggtc ccaacatcgt
     7681 ggagatcgag accgagagcg cgcccggcga gctcaccact atcaacaacc gcgccgtgct
     7741 cacggtggaa ggcgtgcgcg agaacctgcg cgtgctgctg gtttccggcg aaccgcacgc
     7801 gggcgagcgc acttggcgca acatgttgaa gtcggacgct tccgtcgacc tcgtgcactt
     7861 tacgatcttg cgtccgcccg agaagcagga cggcacgccg atcaatcagc tgtcgctgat
     7921 cgctttcccc acacgcgagc tgttccagga gaagctcgat cagttcgacc tgatcatctt
     7981 cgaccgctac cagcgccgcg gcgtgctgcc gctgctctat ctcgaaaacg tctcgcgtta
     8041 cgtggaacgc ggcggcgccg tgctggtctc cgccggcgac gactatgcct ccgctctcag
     8101 cctttaccgc acgccgctcg gtcagatcct gcccgcggtt ccgtcgggac gcgtcctgga
     8161 cgaaccctat aagccggcgc tgaccgatct tggcggcaag catcccgtga ccagcgaact
     8221 gccgggtgcg cgtggcggcg gcccgaacgc cgacgagccg acctggggcc gttggttccg
     8281 agtggtcgat accagcccgc gagaagccga ggtcctcatg aaaggcgccg gcgaccggcc
     8341 gctgctggtc atcggcgagc gcggcaaagg acgcgtggca ctgctcctgt ccgatcacac
     8401 ctggctgtgg gcgcgcggct atgacggcgg cggcccgtat tcgcatctgc tgcggcggct
     8461 ggcgcactgg ctgatgaagg agccggagct tgaggaggag accctcaaag ccaccagcaa
     8521 ggggcaaacg ctcatcatcg agcgccgctc catcgaagac gagatcgacc ccgtcaccat
     8581 caccacgccg tcgggcaagc aagaagccgt caccctcgac aagggcgagc gcggcatctg
     8641 gcgcaagacg atgacggtcg acgagcaagg cgtctatcgc gtggcctcag gcgagctcgc
     8701 cagcatcgcc accgtgggca acgccaacac ccgcgagctt tccgccgtga cggccaccga
     8761 cgcgccgctc tcccccattt tggaggaaac cgggggcggt gccttctggc ttggccgcaa
     8821 agacggcgag gcctccagcc tgccgcggct cgccatggtc cgcagcggcc acgtgatgca
     8881 cggcaccgat tggctcggcc tgcacaaacg cgacgcctat cacgtgaagg gcgtacggct
     8941 cttcccgcta ttcaccggct tcctcgcgct cgcgttgctc ttaggtctcc tggcggccac
     9001 ctggtaccgc gaaggccatt agctagtggt ccgcttgcgc cttgacctgt ccggcggcgg
     9061 cgtccagagc gccgggcacg agctcggcgg ccaacaggcg cgcaggatcg acgggaccgg
     9121 gaagcgcaat ctttccacgc acgaccggca cgaagccgaa gcgctcgtaa tagggcatgt
     9181 cgccgacgag cagcaccagg ccgaagcctt gatccttggc gcgagcgagg ccctcaccga
     9241 ccagcgcctt gccgtaaccg ctgcccttga cgtcgggatc gaccaccagc ggcccgagca
     9301 gcaacccgcc ttcagaatcc ccgattgtga ttgcggtgaa atggatgccg cccacgaggc
     9361 gcccgtccag ccagcccgcg aggctcagcg cggacacagg cggcacgcct tcgcgcaccc
     9421 gatacgccgt ccgcgtgaag cgcccaggcc cgaaagccac ggcagagagc gttgacatgg
     9481 ccgcttcgtc ggcggcggtc tgagagagaa tttcaaaagt tgtcatggcg tttgcaccaa
     9541 atcatcgagg ggaaaatccc tcgccggcca ggcaccaaac gcaagtttcc agagaaagga
     9601 cgaggtgact gcgacgaggc tcaagcggtc cgctgaaagc gggcgcaccc tcgtcgtcgg
     9661 ggaaatgccg aaatttcgat cctcgtacac atgacgggcg gtgccatatc acggacgggg
     9721 ccttgacgta aagcggtcta gcgggcgaag gcccagcaaa gtttacgaac ccgtccaagt
     9781 tcactatgtg ttatcgcctc tcgtcacatt gttgaatctg ctaggctaaa gactgacgag
     9841 acgggggcgt ggagccacac ccctggaggt caatcgggga ctttgtttta tgacggacac
     9901 agcgcaactt ctcgttcttt atcactcatc ctatgggcac accgagacac tggcctatgc
     9961 gatggcgcat ggcgtccgcg cggtcgagga cattcaggtg acggtcaagc gggtgccgga
    10021 gctgatgccc gaggatgtca tgcgcggcgc cggcatgaag gtcgagcagc ctgccgagat
    10081 cgccgacccg caagagctcg ccaactatga cggcatcatc gtgggcacgc cgacccgctt
    10141 cggcaatatg tcctcacagt tgcgctactt cttcgatcag accgggccgc tctgggttgc
    10201 cggcgcgctg atcggcaagg tcggcagtgt cttcacctcg acctcgaccg gcggcggcaa
    10261 cgagaccacc atcgtctccg tccattccac catgctgcac catggcatgg tcgtggtcgg
    10321 cctgccctat tcggcgcccg acctcccgga catcagcgaa gtgaagggcg gctcgcccta
    10381 tggcgcgggc accatcgccg cgccggacgg ctcacgcgtg ccgtcgcaaa aggagctcaa
    10441 cctcgcgagc ttccagggcc accacgtggc caaaatcacc aagaggctgg cgggcggcct
    10501 cgacgactag gcggaccatt tcggactgac cgaagcgcct ccgaaaatct cggcgaggcg
    10561 ctgacgcgta cccgcatcgg tttgcgccgg cacatcgtcg gccgcgaaca tctccgcctc
    10621 ggcgatctcg cccaattgca catggcggcc gcgccgcgtc cacgcgcgga tcagatagac
    10681 cgcgatgtgg tcccccggaa aattcgcgtt gttggagaag atcccatgca gaagcggcgg
    10741 accggtaagc gtcacgccaa cctcctcacc cagctcccgg gccagcccct cttcaagcgt
    10801 ctcgccccac tccacgccgc cgcccggaaa aaaccagcca ggccggtagc tgtgccgcac
    10861 gagcagcaag cggtccgcct cgtcgatcac cacgccttgc gcgccaagcg tcatgccgcg
    10921 ccgcaaacgc cagaaggggc gaagcgttgc cgtgacgagg cgcgaaagcg ccggaccagt
    10981 tgggcgttgg gaactcatgc cttgagcctg taacagcaga gcaccgaaca caatgcctcc
    11041 tgggcacgcc gccaaatttg gcgatcggca aggaggccat tagggtgcgg ctcggtcaag
    11101 cccgcgaaag ccgccgcctg tgttcaccct cgcccacctg tccgacgttc acttgagccc
    11161 gctgccacgg gttaagcgca cggacctcct gtcgaagcga ctgctgggct atgtgaactg
    11221 gcaccgcggg cggaagtttg ttcaccggcg cgaatatctc gatctcctga cccgcgatct
    11281 ggcggagcgc gacccggacc acattgccgt gaccggcgat ctcatcaatc tcggcctccc
    11341 gaaagagtat ctgcgcgcgg cagactggct gcatcactta ggcaacccga acgacgtcac
    11401 cgccatcccc ggcaaccatg acgcctatgt gcgcatgcat cccgatacca gcacgcgcca
    11461 ttggcagccc tacatgacat ccaacgaagc gggcgaagct ctgttcacga caccaacggg
    11521 cgggtttcct ttcgtgcgct gcttcggcga catcggcatc gtcgcgctgt cttcggcgat
    11581 cccgaccatg ccgttcatcg ccgcgggacg cctcggcaaa gcgcaactcg cagatctcgc
    11641 aaacgcgttg gacgcgatgg gccgcgaggg cctctttcgc acggtgctga tccatcaccc
    11701 gccgcttccg ggccaagccg gttggcggcg cggcttgcgc gatgccgatg ccttgcgtga
    11761 cgtgcttgag gcgcatggcg ccgatcttgt cctccatggc cacaaccacg cgcagaccgt
    11821 gcacgagctg caagcggcga gcggccctac gatcgtcgtt ggcgtgccat ccgcctcgga
    11881 cgccgtcgac gggcgcacac cggcggcgcg ctacaacgag tacaacatcg aacgcgccgg
    11941 caatggctgg cgcgtagaga tgatcggccg cgcggccgca gatctagatc aggtttggga
    12001 atgcgagcgg cgcgttctta gggaacgctg agccacactg aggcgaacag gccggcagca
    12061 ccggtgatca cgccgatgat gaacgccgac agcacgatgc ccatgccggg gcccgaactc
    12121 tcaggctcga tcacagtctt ctgacgcggc ggcgcctcca caagtgctgc ttccatccgc
    12181 tcccgccgca cgatctcctg cgccatgtat ttggtgatgc ggtccgccat ctcggcaacc
    12241 gtgttcgcac tgaacaacgt acgccgccca ttctcgctgt tgcgaatgaa cagataggcg
    12301 cccgagccgt cgtcgacggc gacataggta aacaggtcga tccacagtcg cgcaggccgg
    12361 ctcggcgcca gcaccaggct gaagcgatcg tcgcttttcg gaatttcagc gaacacggtc
    12421 tcgagctcgg ccttgagcaa ctccagccgc gcgatctcgc cgtcacgggt gtcggccgtc
    12481 gcgtcgaggc gctccgcctc ctccaggcgc gccttcccga tcgcctcgcg caaggaccgg
    12541 cttgccgccg gacgtgcgat catcttgacg ttgtcgtcca taccagagac gtccctacgg
    12601 ttttcttcct cggattgagg atagcccgcc aagcggcgga aatgcgactg aaattctggg
    12661 ccggattccg gggcaagacg ccttgtttgc ggcgcctcac cggcctcgcc acgggctttg
    12721 ttccgacccc cggccatggc gcatggcccg gcttggcagc gtggcgcgtc ttggcgccct
    12781 cgccccaggg gcggggcggc gccggctcgt tcagaaactt atttggcagc aagtacaacg
    12841 gaggccctcg cgtcgtcgaa ccacttatcg attgcggtgt cgacgctggc ctcaagcccc
    12901 gacctccacg ccactatagt agcctcgaga gcctcttcat tggcagcaac aaagctatcg
    12961 cggttaacgg cttcgcttgc ctcattggcg agatagtcca cggcaatgcc gatcgccacg
    13021 ccgattgcgg cgccgatcac ctgcccgccc acgggctgaa ccgccgtgcc gaccgccgcg
    13081 ccttgaagtg cgggcgccgc acgcgtcgcc agcgacgtcg cgacccgagc cgacatctga
    13141 ccgaagctgc ggctgacaat ggcgttaatg gcacgcccaa gtgtaagcgc gcccaccgtg
    13201 ccgccggccg cggccgcacc cacgcccacg acgccatcga aggcgcggtc ttccatgaga
    13261 tagtgcggcg ctttccactt ctgggcatcc caatcgagct tgacgttggt catggaactc
    13321 gaagccggga tgtcctccat atggctcgtg tgctgagcga ggaagagctg cagccgcatg
    13381 tcgccattgg cgatcacccg ccgatagctg tcatgggcat ggcgcactgc ctgctcgacg
    13441 cccttcgtga tggtctggtc gcggatctcc ggcttcaaca cctgctcggt gtaactctcc
    13501 aggaagtact ctttgacgtc ggcctcgatg gcttcgccca acgactcgta cttgcctgtc
    13561 tcaaggacgc gggaaatcgc cgaagagatg gtctccttca gcacgatata ggaccgcttc
    13621 cattcgaaga accaatcggc atacgcctcg atcgtctcgt cgcggtcttg gaatgccaac
    13681 gcgaacgtac gatcgatgtc gttgcgcgct gcttccttga tgcgcgcccg ctcgtcgtcg
    13741 agcatcagga tggtgtcgtt gacgaactcg tccgtctccg acttgctaac cagaacacgg
    13801 tgcagcttgc cgtcaacatc cttgacaacg agatcgaccg cgtcgggcgg gagcgtccgg
    13861 ctcggcatga agatgtcgaa gatgctggcg tcggggtgca cgggcgccgt gtgatcggcg
    13921 tgccgcgtgt agcttcgata gaaggtggtg cccagtgaca gaattgcata ggcgatcaga
    13981 acgaacccga tcgccatgcc gatgggtttc agaaacatgc caaaggcgct gggcttgacg
    14041 accttttgct taaccgcgcg cgaccgccgc gtcgctttta ccggcgtctc ttcggcgccg
    14101 tgatgttcgg tcacctccga acctgaggtc gtggtcagag ccgtaccctg tcgtggcata
    14161 tcatccatgt ccttgacccc tcgaagctaa gtggaagtgt acgcgcaaca atacccccca
    14221 gccccaggct atatatagtg ttcttcaagg tctcttttaa gatgaatgcg caacatacat
    14281 tcgccagtca cgcacaggct taatgcacaa gttccgcaag ccagtcgaac aaagcttcac
    14341 ggtccgtacg gtattgcgct tggctctcgc cgtcgtcgat cttgatcgag gcgccgccca
    14401 gtgcattgac aacggcaaac gcatcttcat cggtcacatc gtcgcccata tagaccggca
    14461 ggcgtccttt gaagggcggc tcgtcaagga aagccgtgac cgccacgcct ttgtcgcaac
    14521 ctttcggcct cacctcgtag accatcttgc ccggcagaat gacgaggccg tcattatctg
    14581 ccacagcctc ctcaaccgcc gtccggcacg tctccttcaa ctccggccgc tggcgataat
    14641 gcaacgccac ggatgtctgc ttgcgctcca gcagcaggcc cgcattgtct tcgacaaaac
    14701 ctgtaagcgc atcaccaacg gcaatgaccg cctgtaggct gcgcgtgtcg gcatagacat
    14761 ggcctgaagc gtcccgccgg atcgagccat gctccgcggc ggtcgcgagc ctgagcgggg
    14821 caaggaaacc atccaccacg tcgagagggc gcccggtgac gatggcgacc gcgccgtcga
    14881 atcggccgca aagcccccgc agcagatcgc cgacgcgcgg ttccacctcg accagctccg
    14941 ggcgcggggc aagagccgcc agcgtgccgt cgaaatcgaa gaaaaaggcg gaactttcgc
    15001 caagcggcgg caaaggctga gacatatttt tcgcgatcaa gccgtaattt caaggcagta
    15061 gcagctgggg ccacgcattt ggccaccgct taactgctta gatcaattct tcggaggtta
    15121 ggagcccctt gtgacgtttt cagtctaaag tacggcgctc caaccaggat tgaaacatga
    15181 gacatggagg cgtcatccaa agccgctatg cacatgccga cgtctcaagc ctgcatttcg
    15241 gctcgccgcc cacccgccga gaccgcctgc caacgccagg aggcttgaga tgactggaaa
    15301 gacgcggaga cttgttgtcg tctccaatcg cgtcgccgac ttcaacgcga agttccagac
    15361 cggcggctta gccgtcgccc tgggggatgc gctgaaggcg tcgaagggtg tgtggttcgg
    15421 ctggagcggc gacgtgagcg acgacgcgct cgccgaggcg cctaaggtcg agaatatcga
    15481 aggcctcacc gtcgccacca tcgatctcac gcctcaggaa gccgaaggct attactacgg
    15541 ctacgccaat cgctgcctgt ggccggcgct gcactatcgc ctcgatctgg cgcaatccaa
    15601 cgaggagcac agcgaggtct acttcgacgt caacgcgcgg ttcgccgacg cattgatgcc
    15661 gctgatcgaa ccggacgatc tgatctgggt ccacgactat catctgatcc cgctcgccga
    15721 ggaactgcgc ggccggggct gcacgtcgcg cattggcttc ttcttgcaca cgcccttccc
    15781 ttcgccggag atattcgccg ccgtgccggg tcagaagcgt ctcggcaacg cgctcatggt
    15841 ctatgacgtc gtcggcttcc agaccacgtc ggaccgcgac aatttccgcc gctacgtggc
    15901 ggcctcgctc gacggcgaac agcttcatgg cgaccgcatc gccgcgcaag gcgagacgac
    15961 gctcgcctcc gtattcccca tcggcatcga cgccgccgcc ttccgcaagc tggccgagag
    16021 cgaaagcacc aaggacgcga tcctccccga tcttggcgag gacatgaaga cgatcatcgg
    16081 cgtggaccgt ctcgactaca ccaagggctt gccggaacgc cttcgcgctt tcgagacgct
    16141 gctcgaggag ttcccggagc atcacggcaa ggtacgcttg gtgcagatcg cgccgcccac
    16201 gcgcgaaacg cttcccgtct atcaggggat gcgcgaagag gtagagcggc tcgtgggccg
    16261 catcaacggc cgcttcgggg acctgcgctg gacgccggtc acttacctgc accatggcgt
    16321 accgcaggac attttgactc gcctttctcg cgagagcgcc gtcggtctcg tcacgccctt
    16381 gcgcgacggc atgaacctcg tggccaagga gtacatcgcc gcgcaggcgc cgcaggaccc
    16441 cggcgtgctt gtcctgtcgc gcttcgccgg ggcggcggag cagctgcgcg aggcactcct
    16501 catcaatccc catgacgtca acgagttggc gcgcgccctg gatcgcgccc tgaccatgcc
    16561 ccgccaggag cgctgggcgc ggcacaaccg gctgtggcag cggatcgaga cctacgatct
    16621 cgccaattgg cgcgacagct tcatccagac ctttgccgag tccgacacag accccgtgaa
    16681 tctgtttgcc aagaaggccg gcgccgccta agagccagcc aaggctcgct ggcggcaaga
    16741 cccaccgccc ctcaaggctt gggggcgcgg ccgatagcct ctggtcgagc gcgcgtaagc
    16801 cgcttaactt ctgtcgacgt accgcctatg taggcgccct tcgagatgtc accgccccgc
    16861 ccctagtcgc tattccccag tcatgatcga tcgcaccgac cggctctggt acaagaacgc
    16921 cgtcatctac gagctgcaca ttaaggcgtt cttcgattcc gacaatgacg gtatcggcga
    16981 tttcgcaggc ctcatgcgga agctcgacta tctccaagag ctcggcgtca ccgccgtctg
    17041 gctcctgccg ttctacccct cgcccttgcg ggacgacggc tacgacatcg ccgactacac
    17101 ctcgatcaac cccacctacg gcaccatgcg cgacttccgc cagttcgtgg cggccgccca
    17161 tgaccgcggc attcgcgtca tcaccgagtt ggtggtcaac cacacctccg accagcaccc
    17221 ctggtttcag cgggcgcgca aggccaagcc gggctctgtg tggcgcgatt actatgtgtg
    17281 gagcgacacg gaccagaaat atcccggcac gcgcattatc tttctcgaca ccgagacctc
    17341 gaactggaca tgggacccgg aggccaaagc ctatttctgg caccgcttct actctcacca
    17401 gccggacctg aactacgaca acccgcgcgt cctcgcggac atcttgcggg tgctgcgctt
    17461 ctggctcgac atgggtgtcg atggcctcag gctggacgcc gtgccctatc tcgtcgaacg
    17521 cgagggcacc aataacgaga acctgcccga gacccacgag ctcctgaaaa agttccgcgc
    17581 ggagctcgac cagcgctatc cggaccgcat gctgctcgcc gaggccaatc aatggccgga
    17641 ggatacgcgc ccctatttcg gcgacg
//
LOCUS       DBNHVU010000155         9163 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_946514, whole genome shotgun sequence.
ACCESSION   DBNHVU010000155 DBNHVU010000000
VERSION     DBNHVU010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 9163)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9163)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9163
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_946514"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1178)
                     /locus_tag="AC16Y7_06285"
     CDS             complement(183..1178)
                     /locus_tag="AC16Y7_06285"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HIR5709024.1"
                     /translation="MADQLPPPPARTLEHVAVLMGGWSSEREISLKSGTAVASALEAE
                     GYRVTRIDVDRSIAGVLGELEPDVCFNALHGPIGEDGSIQGVLEVLGIPYTHSGVTAS
                     ALAMHKTHAKAVMAACAGVPVAEGMEVTRRKAAMAHVMAPPYVVKPIADGSSFGVVIV
                     PEGATATPPEIGGGGAANEIVMVERYIPGRELTCAVIGDFVTDVIEICPEGGHGFYNF
                     DAKYAPGGSRHVLPAEILPEIYQLVRKYTLAAHRALGCRGVSRADFRLDESHQSNQAD
                     RAGHGLICLEVNTQPGMTATSLVPELAAHAGWSFGDLVRWIVEDARCSRCMDKAL"
     gene            complement(1273..2253)
                     /gene="murB"
                     /locus_tag="AC16Y7_06290"
     CDS             complement(1273..2253)
                     /gene="murB"
                     /locus_tag="AC16Y7_06290"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR5709025.1"
                     /translation="MSFPDITEEIRAKMPELRGSLAGNRSLADITWFRVGGPAQVLFT
                     PADEADLAYFLKQNGGALAVEVIGLGSNLLVRDGGVPGVVIRLGRGFGHVAAEEKCRV
                     RAGTVVPDVKVARVAAEAGITGLSFYRGIPGSVGGALRMNAGAHGGETKDVLVEARAV
                     APDGTVHVLSLEDMDFSYRHSGVPDNWIFTEALFQGQPGDLEKIAQDMKEVAKYREEN
                     QPIKERTGGSTFKNPPGHSAWKLVDAAGLRGYRVGGACVSSMHCNFLINDQGATAEDL
                     ERLGETIRARVKETSNVELHWEIKRIGVPLPGHATGEALAEPRAAQEVAR"
     gene            complement(2250..3689)
                     /gene="murC"
                     /locus_tag="AC16Y7_06295"
     CDS             complement(2250..3689)
                     /gene="murC"
                     /locus_tag="AC16Y7_06295"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR5709026.1"
                     /translation="MQMPRDAGTFHIIGIGGIGMSAIAEILMAKGYQVQGSDQKESAN
                     VRRLKAKGIRVFVGHDPINLVGAENVVISTAVKPDNVELAAARQRGLTIIRRAEMLAE
                     LMRLYSTVSVTGTHGKTTTSSLLAHVFESADLDPTVITGGILNNWGSNARLGQGRWMV
                     VEADESDGTFTRLPTEIGVVTNIDPEHLDYFKSVENMHREYAAFLRNIPFYGLGVACS
                     DHPVVREMIEKLGLRKDGRRLLTYGTGEDADLRLVHARADGHHTVFDFEVSERVAGGA
                     RPLTGWTIPILGQHNVLNALAAVAVALDAGVEAEKVQAGLASFAGVKRRFQLTGSWRG
                     VSFFDDYAHHPVEINAVLAAAKGGARGRVMAVVEPHRFTRVRDLFGEFCNCFHSADSV
                     IVLPLYSAGEFPIDGIDQFSLAEGIRQTGHPAAIAVENVEQLVGLIAEHARSEDMVVF
                     VGAGQSTEWAHAVPKWLNEREPMRAGGAV"
     gene            complement(3805..4902)
                     /gene="murG"
                     /locus_tag="AC16Y7_06300"
     CDS             complement(3805..4902)
                     /gene="murG"
                     /locus_tag="AC16Y7_06300"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013945980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR5709027.1"
                     /translation="MKDGKTILLAAGGTGGHLFPAFALAEELGKRSYAVDLATDMRGD
                     RYGTSFPARTVYRIRSATLRGKSPLAVAITGLALARGTWAARKILRDVKPAVVVGFGG
                     YPTFPPLLAAKAAGVPAVLHEQNAVLGRANKMLAHHVQRIAISFPETKFLKAEWADKV
                     RVVGNPVRALVLAAAAHDYPELRPDGPFRIVVFGGSQGARMLSDRVPEAVAGLPEALK
                     ARLHVVQQAREEDVQRVREAYERAGISAEIAHFFADLPDKISRAHLVVARSGASTVAE
                     LTVIGRPAILVPLPGSLDNDQLLNATQLAESGAAWCIEQKDFSIQRLSGELERLASAP
                     ETLNAAARAARRLARPQAVADLADVVEELAA"
     gene            complement(4902..6080)
                     /locus_tag="AC16Y7_06305"
     CDS             complement(4902..6080)
                     /locus_tag="AC16Y7_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR5709028.1"
                     /translation="MMFSRADRSRFVDWWFTVDHALLAAFFTLIGLGVILSLAASPSV
                     AASKGLPAYFFVQRHLLFAAIACVVMLAVSLLEPAQVRRLAFVLFLMSLVLLVTVYWS
                     GREINGARRWLSIFGYSFQPSELAKPAFVIVSSWFFAEARHNKEMPGLIFALASASLF
                     LMLLVVQPDVGQTVLAASIWGVLYFVSGQPLVGGIVLFLFGVIGLITAYVVFPHVQSR
                     IDRFIWPDQGDNSQIDRAMQSFTEGGFFGRGPGEGTIKTVLPDAHTDFIFAVVAEEYG
                     VVACLIILALFTFAVLRALRRARYEQVVFIRLSIIGLALLIGLQAYINMGVNIGLLPA
                     KGMTLPFISSGGSSILAVSLTAGMLLALTRRRPDYVGRNKPAPTITVGGVQSPAGKFR
                     "
     gene            complement(6077..7537)
                     /gene="murD"
                     /locus_tag="AC16Y7_06310"
     CDS             complement(6077..7537)
                     /gene="murD"
                     /locus_tag="AC16Y7_06310"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR5709029.1"
                     /translation="MIRCTSFRNKTVAVFGLGGSGLAAAKALAAGGADVVCWDDGEAG
                     REAALKAGLFVKDLEQHNWREFAALVLSPGVPLTHPKPHWTVEKAKAASVEIIGDVEL
                     FARERDLVAPGSPWIAITGTNGKSTTTALTAHLLDSLGCDVAMGGNIGKAILALATPA
                     MDRVHVVEMSSYQIDLSPTARATVGVLLNITPDHLDRHGTIDNYAAVKARLVERADAA
                     CICVDDAWTREIAAQITPVSKLYAFTSGKGAAIVPKFYAIGASLFVHDKQDGFAESRM
                     IADLTQSMALRGAHNAQNALAALAAIRALADQLQDSNPALAEKVWNVDALCRGLMSFP
                     GLAHRLQPVASLDGVTFVNDSKATNAESTEKALLSFDGNIYWIAGGRAKDGGIEALRP
                     LFGRVARAYLIGEAAEDFAVTLEGQCAHRICGTLEQAVTEAARDAKTEGRKGAVVLLS
                     PACASFDQYANFERRGEHFCQVVSALQGVSMHEGVA"
     gene            complement(7607..8842)
                     /locus_tag="AC16Y7_06315"
     CDS             complement(7607..8842)
                     /locus_tag="AC16Y7_06315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5709030.1"
                     /translation="MKKVLIACPQCDGQDVGENLCGFEWTSRLAREADVTLLTLRFPG
                     HEAPSKQLKGVRVVEWGAKPYLASRPRVNSTVKPWYPHFYYQARRWTKAALQGGEQFD
                     LLHQIMPMATRYPSPFSGLEVPFILGPVGGGVATPDGFRGELGSEPPFMRLREFDRMR
                     LRRDPMLRKTYQRAAAVIGCSPYVEEHLKDIKLKRFVSEYEVGIEELPAAATRDGGPS
                     GPLRLLFVGRIIRTKGLRDAIRALAKLNDVDDVRLEVAGSGEDVEPCRTEAAALGVGD
                     RVRFLGRLPRGELQPLYAKADVFLFPSFREPTGGVLFEAMQFGVPVVTSTLGGPGHIV
                     TEACGIRVAPNSPEQFAEDIAAAIRHLALNPDLRSKMAAASRDRIAELGLWERKLGRI
                     LALYDDVAGDAGEDEGFAA"
     gene            complement(8983..>9163)
                     /gene="mraY"
                     /locus_tag="AC16Y7_06320"
     CDS             complement(8983..>9163)
                     /gene="mraY"
                     /locus_tag="AC16Y7_06320"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003603658.1"
                     /note="First step of the lipid cycle reactions in the
                     biosynthesis of the cell wall peptidoglycan; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR5709031.1"
                     /translation="MSVVIQVVSYKLTGRRVFRMAPLHHHFEQKGWHESTVVVRFWII
                     SVVLALIGLASLKLR"
BASE COUNT         2086 a         3070 c         2372 g         1635 t
ORIGIN      
        1 gcgccggcgc agcacgtgtc catgcggagc aattcgaaac gccggaaaat tgtcgccaag
       61 tggccggttg cgaagcggag actgggccaa agactggggc agagattggg ccagagactg
      121 gggcggactt tgtcgagaaa ctcggcgtag aggtttcccc acctgatcag ggcggtgctc
      181 agttataaag ccttgtccat gcacctgctg caacgggcat cctcgacaat ccagcgcacc
      241 aaatcgccaa acgaccatcc tgcatgggct gccaattcgg gaaccaagga cgtcgccgtc
      301 atgccgggct gtgtgttcac ttccaaacag atgaggccgt gtccggccct gtctgcctgg
      361 ttgctctggt gtgactcatc caatcgaaaa tcagcgcgtg aaacgccccg gcatccgagt
      421 gcgcgatgag ccgccaatgt gtatttccgg accaattggt aaatttcagg taaaatttcg
      481 gccggcagca cgtgccgcga cccgcccggc gcatacttgg catcaaagtt gtagaagcca
      541 tgaccgcctt ccgggcagat ctcgatgacg tcggtgacga agtctccaat caccgcacac
      601 gtcaattcac ggcccggaat atagcgttcg accatcacga tctcgttcgc cgccccgcca
      661 ccgccgatct caggcggcgt tgcagttgcc ccctcgggca caatgacaac cccgaagctc
      721 gaaccgtccg cgatgggttt gacgacgtag ggcggcgcca tcacatgcgc catggccgct
      781 ttccggcgcg tcacctccat gccttccgca accggtaccc cggcgcacgc tgccatcacg
      841 gctttggcgt gtgtcttgtg cattgcaagc gccgacgccg tcacccccga atgcgtatag
      901 ggaatgccaa gcacctccag gacgccctgg atcgagccgt cttcaccaat cggaccgtgc
      961 agtgcgttaa aacaaacgtc cggctcaagt tcgcccaaca cgcccgcaat cgaacgatcg
     1021 acgtcgatcc gcgtcacccg gtagccttcc gcctcgagcg cactggcaac agcggttcca
     1081 gatttcagac tgatctcacg ctctgacgac cagccaccca tcaaaacagc aacatgctca
     1141 agcgttcggg caggcggtgg cggcaattga tcggccatca acaaactccg ggtaaccgct
     1201 tagcagcgga cctaaagaca acaatcgtca gcttatctgc tgccatgaaa gttctacgcc
     1261 gcgccacatt gatcaccgcg ccacttcttg cgccgcccgt ggctcagcca aagcctcgcc
     1321 cgtggcatgg ccaggcaaag gcacgccaat gcgctttatt tcccaatgca gttcgacatt
     1381 cgaagtctct ttgacgcgcg cacggatcgt ctcacccagc cgctccaggt cctcggccgt
     1441 cgcaccttgg tcgttgataa ggaaattaca atgcatcgat gaaacacacg ccccgccaac
     1501 cctgtagccc cgcaggcccg ccgcatcgac cagcttccag gcgctatgac cgggagggtt
     1561 cttgaacgtc gaaccgccgg tgcgctcttt gatcggctgg ttttcttcgc ggtacttcgc
     1621 cacttccttc atgtcctgag cgatcttctc aaggtcgccc ggctggcctt ggaagagggc
     1681 ctcggtgaaa atccaattat ccggcacgcc tgagtggcga taggaaaagt ccatatcctc
     1741 cagcgacaac acatggacgg ttccgtccgg tgcgacggcg cgcgcttcga caagcacgtc
     1801 cttcgtctca ccgccatgag cgcccgcatt cattctgagc gcgccgccga cagaaccggg
     1861 tattccgcga taaaacgaaa gaccggtgat gcccgcctcc gccgcaaccc gcgccacttt
     1921 cacatccgga acaaccgtgc cggcccgcac cctgcatttc tcttccgccg caacatggcc
     1981 gaaaccgcgg ccaaggcgaa taacgacacc cggcacgccg ccatcgcgca ccaacagatt
     2041 agagccaagc ccaatcactt cgacggcaag tgcaccgcca ttctgtttta gaaaataggc
     2101 aaggtcagct tcatcagcgg gcgtaaaaag cacctgtgcc ggaccgccaa cccgaaacca
     2161 tgtgatatct gccaaactcc ggttgccagc gagactgccc cgcagctccg gcatcttcgc
     2221 gcgaatttcc tccgtgatgt ccggaaagct caaacggcac cccccgcacg catcggctcg
     2281 cgctcgttca gccacttcgg aacagcatgc gcccattccg tcgattgtcc agcaccgaca
     2341 aacaccacca tgtcttcgct gcgagcatgt tcggcaatca ggccgacaag ctgttcgaca
     2401 ttctcaacgg cgatcgccgc cggatgcccg gtttgacgaa tcccttccgc aagcgaaaac
     2461 tgatcgatgc cgtcgatcgg aaactctccc gccgaataaa gcggcagaac aataacgctg
     2521 tcagcgctgt gaaaacagtt acagaattcg ccaaagagat cgcgcacacg cgtaaagcgg
     2581 tgcggctcga caaccgccat cacccggccg cgcgccccgc ccttggcagc cgccagcaca
     2641 gcatttatct ccaccggatg atgtgcatag tcatcgaaga acgagacgcc gcgccacgag
     2701 cccgtcaatt ggaaacggcg tttgacgcca gcgaagctcg caagtcccgc ctgcactttt
     2761 tccgcctcca ccccggcatc caacgctacg gccactgccg ctaacgcatt caagacgttg
     2821 tgctggccaa ggatcggaat cgtccaacct gtgagcggcc ttgccccgcc cgcgaccctt
     2881 tcgctgacct cgaaatcgaa tacggtatgg tgtccgtccg ccctcgcatg caccagccgc
     2941 agatcagcgt cctcgccggt gccataggtc aaaagccgcc gcccatcctt gcgcaagcca
     3001 agcttctcga tcatctcccg cacgacaggg tgatcgctac acgcaacgcc aagtccataa
     3061 aacggaatgt tgcgcaggaa cgccgcatac tcgcggtgca tattctcaac gctcttgaaa
     3121 tagtcgagat gctcgggatc gatatttgtc acgacgccga tttccgtcgg caatcgcgtg
     3181 aacgtgccat cgctttcgtc agcttccaca accatccagc ggccttgacc gagccgcgcg
     3241 ttgctgcccc aattgttcaa aatcccgcct gtgatcaccg ttggatccaa atcggccgat
     3301 tcaaacacat gcgccaacag cgatgaagtc gtcgtcttac cgtgcgttcc tgtcaccgag
     3361 acggtcgaat aaaggcgcat cagttcagca agcatttccg cccggcggat gatcgtaagt
     3421 ccgcgctgac gcgccgccgc cagctcgaca ttgtcaggct tgacggcggt tgaaatgacg
     3481 acgttctccg cccccaccag attgatcgga tcgtgtccga cgaaaacgcg gatacccttg
     3541 gccttgagac ggcgcacatt cgccgactcc ttctggtcgg agccctggac ctgatacccc
     3601 ttcgccatca gaatttcagc gatcgcgctc atgccgatgc cgccaatccc gatgatatga
     3661 aatgtcccgg cgtcgcgagg catctgcata gttttaacgt tcctgttcaa aggcgcctga
     3721 agcggcgcag aatttagaca cgatgcctct tttacgcgcc gctcgccatc cggagtggtg
     3781 aagaaacgtg gtgacctaaa acggttaagc cgccagctct tcgaccacat cggccaaatc
     3841 ggccacggcc tgaggccggg caaggcgccg cgccgcgcga gccgccgcgt tcaaggtttc
     3901 cggagccgat gccagccttt caagctcgcc gctcagccgt tggattgaaa aatccttctg
     3961 ttcgatacac caagcggccc ctgattcggc aagttgcgtc gcattcagca attggtcgtt
     4021 gtcgagcgaa cccggcagcg ggaccaggat tgcaggccgt ccaataaccg tcagttccgc
     4081 caccgtcgag gctccgctgc gcgccactac cagatgggca cgactgatct tgtcaggcaa
     4141 atcggcaaaa aaatgggcga tctcggcgga aatccccgcc cgctcgtagg cctcgcggac
     4201 gcgctgaacg tcctcttccc gtgcctgctg gacgacgtgc agcctcgctt tcaaggcctc
     4261 cggcaagccc gcaacagcct ccggcacccg gtccgagagc attctggcgc cctggcttcc
     4321 tccgaaaacg acaatccgga acggaccatc cgggcgaagt tcagggtaat catgggccgc
     4381 cgccgccagt acgagagccc gcacaggatt gccaacaacg cgcaccttgt ccgcccactc
     4441 cgctttcaag aacttcgttt ccggaaacga aatcgcaatc cgctgcacat gatgagccag
     4501 catcttgttg gcccgcccga ggacggcatt ttgttcgtgc agcacggctg gaacgcccgc
     4561 cgcttttgca gccagaagag gaggaaaggt tggatagccg ccaaagccaa ccacaaccgc
     4621 cggcttgacg tcgcgcaaaa tcttccgcgc cgcccacgtt ccacgcgcca gcgccagacc
     4681 cgttattgcc accgccaacg gagacttccc tctgagcgtc gccgaccgaa tacggtagac
     4741 ggtgcgggct gggaaactgg tcccgtaacg gtcgccgcgc atgtcggttg caagatcgac
     4801 ggcgtaggaa cgctttccca attcttccgc cagcgcaaac gccggaaaca aatgcccgcc
     4861 ggttccaccc gctgccagca ggatcgtctt gccgtctttc atcaccgaaa tttccctgcc
     4921 ggagactgca ccccaccgac agtgatggtg ggagcaggct tgttgcggcc gacatagtcc
     4981 ggcctgcgcc gcgtcagcgc aagcagcatg cctgccgtca acgacaccgc gagaatagac
     5041 gacccgccgg atgaaatgaa cggcaacgtc atgccctttg ccggcagcag tccgatgttc
     5101 acgcccatgt tgatataggc ctgcaatcca atcaaaagcg ccagaccgat gatgctcaat
     5161 cgaatgaaga cgacttgttc atagcgtgcc cggcgcaacg cacgcagcac cgcaaacgta
     5221 aagagcgcta gaataatcag gcacgcgacc acgccatatt cctccgccac gactgcgaaa
     5281 atgaaatcgg tgtgggcatc gggcagcacg gttttgattg tcccttcgcc cggaccgcgt
     5341 ccgaaaaatc cgccttccgt aaaactctgc atcgccctgt caatctggga gttgtctccc
     5401 tgatccggcc agatgaaccg gtcaatccgc gattgcacat gcggaaagac cacataggcc
     5461 gtaatgagac cgatgacgcc gaacaggaac agaacaatcc ctccaaccag cggctgtccc
     5521 gaaacaaagt agagcactcc ccaaatgctg gcagccagaa ccgtctgccc gacatcgggc
     5581 tgcacaacaa gcagcatcag aaacagtgaa gcagacgcca gggcaaaaat gaggcccggc
     5641 atctccttgt tgtgtctggc ctcggcaaag aaccatgacg acacgatcac aaacgccggc
     5701 tttgccagct cagacggttg aaacgaatag ccgaaaatcg aaagccagcg ccgcgctcca
     5761 ttaatctcac gcccgctcca ataaaccgtc acaagcagga ccagagacat cagaaacaaa
     5821 acgaacgcca aacggcgcac ctgagccggc tctagcagcg acacagccag catcacgaca
     5881 caggcaatag cggcgaataa cagatggcgc tggacaaaaa agtaggctgg caagcccttg
     5941 ctggctgcaa ccgacggact ggcagcaagc gacaagatga cgccaagccc gatcagagtg
     6001 aaaaaggcag ccaatagcgc atggtcgacc gtaaaccacc agtcgacaaa ccggctcctg
     6061 tcagcgcgcg aaaacatcat gccacacctt cgtgcatcga aacgccttga agcgccgaca
     6121 ccacttggca gaaatgctcg ccacgccgct caaagtttgc gtattgatcg aacgatgcgc
     6181 acgccggaga caacaaaaca accgcaccct ttctcccttc cgtcttcgca tcccgggccg
     6241 cctccgtcac agcctgctcc aatgtcccgc atatgcgatg agcgcattgc ccctcgagcg
     6301 ttactgcaaa atcctccgcc gcctctccga tcagataggc ccgtgcaacg cggccgaata
     6361 agggacgaag cgcctcgatt ccgccgtctt tcgcgcgccc gccggcgatc caataaatgt
     6421 tgccgtcgaa actaagaagg gccttttccg tgctctcggc attggtcgcc tttgagtcgt
     6481 tgacgaacgt gacgccatca agggacgcaa cgggttgcaa tcggtgcgca agacctggaa
     6541 acgacatcaa cccccgacaa agcgcatcca cattccagac tttttccgcc agcgccgggt
     6601 tagagtcttg aagttggtcc gctaatgccc ggatggccgc cagcgccgcc aacgcattct
     6661 gcgcgttgtg cgcaccgcgt agcgccatcg attgggtcaa atccgcaatc atccggcttt
     6721 cagcgaagcc atcctgcttg tcgtgaacaa acagcgaagc gccgatggca taaaatttcg
     6781 gaacaatggc cgcgcccttg cctgaggtga acgcatagag cttcgaaaca ggcgtgatct
     6841 gcgccgcaat ctctcgcgtc caggcatcgt cgacacagat gcaggccgca tcggcccttt
     6901 cgacaagccg cgctttcacc gccgcataat tatcaatcgt cccatgccgg tcgagatgat
     6961 caggcgtgat gttaagaaga acaccaactg tcgcccgcgc tgtcggcgac aagtcaattt
     7021 gataagacga catctcgaca acatgcacgc gatccatcgc cggcgtcgcc aaagccaaaa
     7081 tcgccttgcc gatgttgcca cccatcgcca cgtcgcagcc aagcgagtcc agcaagtgcg
     7141 ccgtcagagc cgtcgtcgtc gacttgccgt tggtgccggt aatcgcaatc caaggcgagc
     7201 ccggtgcaac aaggtcccgc tcgcgggcga acaactcaac gtcaccgatt atctcaacac
     7261 ttgccgcctt ggccttttcg accgtccaat gcggctttgg atgcgttaaa ggaacgcccg
     7321 gcgacaaaac gagagcagca aattcccgcc agttgtgctg ttcgaggtct ttaacgaaaa
     7381 gccccgcttt cagggcggct tcacgccccg cctctccgtc atcccagcac acaacatccg
     7441 ccccgccggc agccaacgcc ttggcggccg ccagccccga cccgccgagc ccaaaaacag
     7501 cgacggtttt attacgaaag gacgtacagc gaatcatcgg acgattagaa catgcaaaag
     7561 aggaggggcg gaacttggct gcttgtcacg gttgctcttc gccccgtcaa gcggcaaaac
     7621 cttcatcctc tcccgcatca cccgccacat catcgtaaag ggcgagaatg cggccaagtt
     7681 ttcgctccca aagccccaac tccgcaattc ggtcccgcga cgccgccgcc atcttggaac
     7741 gcaagtcggg gttcaacgcc agatgccgaa tggcggcggc aatatcctca gcaaactgct
     7801 ccggcgaatt gggggcgacg cgaatgccgc aagcctccgt cacaatgtgg cccggtccgc
     7861 caagcgtcga cgtcacaacc ggaacgccga actgcattgc ttcgaacaag acgccccccg
     7921 tcggctcgcg aaaactcggg aacaagaaca catccgcctt ggcataaagc ggttgaagtt
     7981 cgccccgcgg caagcggcca agaaaccgaa cccggtcgcc aacccccaac gccgccgcct
     8041 ctgtccggca tggctccaca tcttcccccg aaccggcgac ctcaagtctc acatcgtcaa
     8101 catcgttcaa cttggcaagc gcccgaatgg catctcgcaa ccccttggtc cggataatcc
     8161 ggccaacaaa aagcaatcgt aacggcccgg atggcccgcc atcgcgcgtc gccgctgcag
     8221 gcagttcctc aatccccacc tcgtactccg aaacgaagcg tttgagtttg atgtctttga
     8281 gatgctcttc gacataagga ctgcagccaa tgacggctgc agcacgctga taggtcttgc
     8341 gcagcatggg atcgcgccgc aacctcatgc gatcgaactc cctgagccgc atgaacggcg
     8401 gttcgcttcc cagttcgccg cgaaagccgt caggcgtcgc aacaccgcca ccgacggggc
     8461 ccaagatgaa gggcacctct aatcctgaaa acgggcttgg atagcgcgtt gccatcggca
     8521 tgatctgatg cagcaggtcg aactgttcgc cgccttgcag cgccgccttc gtccaccggc
     8581 gcgcctgata ataaaaatgc ggataccagg gctttaccgt gctgttgacc cgcggccggc
     8641 tggcaagata gggcttggca ccccattcaa ccacacgcac acccttgagc tgctttgacg
     8701 gcgcctcgtg tccgggaaac cgcaaagtca gcaacgtaac gtcggcttct ctggcaagcc
     8761 gcgaggtcca ctcaaacccg cacagatttt ccccgacgtc ttggccgtcg cactgcgggc
     8821 acgcaatgag tacctttttc atatcagcaa tgggcctttg ccatccttgc ggctcaccga
     8881 tcccgccaga cgaaccccgg caaaaccaaa tttaccgccc ttcctccctc gcatcacaaa
     8941 ggggagaaga acaacgcaaa cgtcttgctt tacagaaaaa tcctaacgca acttgagaga
     9001 cgccaggccg atcagtgcca acaccacgga aataatccag aaccggacca ccaccgtact
     9061 ttcgtgccag cccttctgtt cgaagtggtg atgaaggggc gccatgcgga acaccctgcg
     9121 cccggtcaac ttataagaga caacctggat caccaccgac att
//
LOCUS       DBNHVU010000156        11181 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_427513, whole genome shotgun sequence.
ACCESSION   DBNHVU010000156 DBNHVU010000000
VERSION     DBNHVU010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 11181)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11181)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11181
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_427513"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1279
                     /locus_tag="AC16Y7_06325"
     CDS             245..1279
                     /locus_tag="AC16Y7_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_function: GO:0015369 - calcium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA];
                     GO_process: GO:0070588 - calcium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium:cation antiporter"
                     /protein_id="HIR5709032.1"
                     /translation="MDSTAWLALMFAWLFGVMIWCAFGVVRHADGLAEILGEPYGTLI
                     LTISVISMEVAILATVMLSGSPNPTLPRETMFAILMIVMNGMVGTVLAVGGLRHVVQQ
                     YNLQGALAYLAVITPLAVLALVLPTFTKSTSGPTLDTQQAIFLGTLTALLYVAFLTIQ
                     TTRHRTFFVQPSRKAASSERPAPASPEPHHHGPVYSGPYHAVLLIATLLPVVLLSKPL
                     ASILDHGIDQLGLPTALGGVLIAVLILSPEWSAAIGAAVRDQLQRAVNLSLGSALSTI
                     GLTVPVVLAISVLTGTPLELGLNAVDMVLLAVTLVVCMITFSGAPTNILLGFVHLVLF
                     AAYLMLIFKP"
     gene            complement(1717..2859)
                     /locus_tag="AC16Y7_06330"
     CDS             complement(1717..2859)
                     /locus_tag="AC16Y7_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5709033.1"
                     /translation="MTVALSLGATANLGHAQNTLNVATWGGAYGQSQEAAIFEPFAKE
                     TGVSIDTVTYDGRLAKLKTMLNDDGTVVDVIDVSADALNTLCKQGLLETIDASALGAG
                     PDGSAAKEDFLPGALSPCGVASVAWSTAIAFDRKAFPKGAPTAISALLDTAGFPGIRA
                     LPNNARYSLELALLADGVPPENVYRELATPEGADRAFAALDRITGAVRLWNKAQQAIT
                     WLVEDDVVMAASYSGRLFRAAAGDRNIDILWDGQIYDFDAWAIPKSSDNKKDALRFIR
                     FATVPARLADQAELTAYGPMRKSAFPLVGKHPVINVEMLEFLSTAPQNFQKALKFDAA
                     WWRGNGAALQKRFAAWRARVNAARTDREAARKAKEAAEKEADKAAP"
     gene            complement(2946..4742)
                     /locus_tag="AC16Y7_06335"
     CDS             complement(2946..4742)
                     /locus_tag="AC16Y7_06335"
                     /inference="COORDINATES: protein motif:HMM:NF017021.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR5709034.1"
                     /translation="MKPRQLPRKWFLIGLLVLLIAGAVALPRQIGTSSQLRDRIGAVL
                     SEWSGGTITLTEPLRIRYFPPLSLRGGFILTNATKLPGIGAITAPDVEFSLDFADLLL
                     GRTKIDALRISKPVITLKETDAAAPEDSPISGALVDPPLGAVRMRGGTIKNAYGVPIV
                     KDFEVRLDPKGRRGALAALGSFHYRGETVAFAMDTGTVAETEDGTLAPVTLKVTADGA
                     KAEFSGKVRAADRLEAEGELEAEMSDARHVLNWLGMSLPKGESLHDVKGSGMAHWSGT
                     TLTFDRGAFSLDGNEAVGLLVVTAGDRPRIEGTLDFERLVLDPYLGTSEAAKDRLFDR
                     AILRYLDADLRLSAAEIAALTLELGRGGLTLTAKDGAISSEIGALELCGGLATGRVSL
                     NLSEARPEASLAGNLSDAAVETCLAQFDLDVPIKGTGVLKVDVSTGGETRDELIRNLV
                     GKFEVAAKDGAVPIDLSALASGAGEGKAGWSQDATTAFETLDANCQLTAGHVWCQSFT
                     MQTPQGTISGSGGVDVGKQTIDWNFLIANPVTPLNASQLVMESPPRVTIRGPLTQPEV
                     RGADRPAPADASSQPSSESGSAAPSTQNAPAD"
     gene            complement(4904..5665)
                     /locus_tag="AC16Y7_06340"
     CDS             complement(4904..5665)
                     /locus_tag="AC16Y7_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5709035.1"
                     /translation="MLPAAVLVSDAVAHISSGLSPAFWQAAIHSLGLSVAAAVLAVSF
                     AVVLAYARRQTRSKLVHAASLAPAVSYAVPGTVLAIGLLIPLAGLDNRLDGVMRELFG
                     VSTGLLLSGTAFAIVLAYTIRFLAASLGAVEAGMSKISRNIDAAGRTLGASIGEMLWS
                     VHLPLLRPALGAAAMLVFVDSMKELPATLLLRPFNFDTLATQVFTLASLYRYEEAGLS
                     ALAIVLVSLAPVLLLHGMISRSRPGASVGKMIGVE"
     gene            complement(5750..6733)
                     /locus_tag="AC16Y7_06345"
     CDS             complement(5750..6733)
                     /locus_tag="AC16Y7_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5709036.1"
                     /translation="MRFKRPISVSPRKRSAPSWQRAPAARTVSLASAEQHTADSRPLE
                     TVSFRRKLARLTESGNLGWSVAALVFSAIALAPIIAIAVIALQPSGDTWPHLIANVLP
                     GAVRRTLGLMLGVGLLTLLIGTGTAWLVTMYRFPGRRYFQWLLLLPLAMPTYIIAYCY
                     LELFDYSGLVQTGMREAFGWRNAQDYWFPDIRSLGGAIFFMSMVLYPYVYITARASFI
                     AQSICVIEVSRTLGRSASETFWQIALPLARPALAAGVALALMETLNDIGAVEFFGVHT
                     LTVAVYDTWLDRGSLAGATQIACVMLTFVFVLLALERALRSGRRFHNTTGK"
     gene            complement(6633..7154)
                     /locus_tag="AC16Y7_06350"
     CDS             complement(6633..7154)
                     /locus_tag="AC16Y7_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709037.1"
                     /translation="MSIHKEIDARNGRQAVVSPRTGAEWLVGVGFRCWLAGYDTGDIG
                     CWEKGWNEYNRVLGASRAKRAVTELACWVRAVRSSASRKIQYYPLARPGFCNDECMAI
                     SLIAASQHHRCPAMQACALALTGSDLVEPVIDAANAFADALQEADQRLSPEAVSALMA
                     ARTGREDGLAGQR"
     gene            complement(7165..8211)
                     /locus_tag="AC16Y7_06355"
     CDS             complement(7165..8211)
                     /locus_tag="AC16Y7_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5709038.1"
                     /translation="MRWTLACAAALATLSLAFAPSIARAGNPGEVNIYSYRQPYLIEP
                     LLKAFTAQTGIKTNIIFASKGLIERIQAEGRNSPADVLLTTDIGNLSQAAASGIAQPI
                     TSETNTAAIPAAYRDEDSAWVGLTRRGRVVYASKDRVTQDTITYEELAEPKWRGKICI
                     RSGQHPYNIALIASMIAAHGEDWTEEWLRGVKSNLARKPAGNDRLQVKGVYAGECDLA
                     IGNTYYMGKMLTDEKHPEQKEWANAVNILFPNTKDRGTHVNLSGAVLAKNAPNKENAI
                     KLVEFLASDDGQEMYAEVNHEYPVKDGVPWSGLVQSWGTFKSDPISLNEIAALRKTAS
                     ELVDKVGFNEGPSS"
     gene            complement(8529..9905)
                     /locus_tag="AC16Y7_06360"
     CDS             complement(8529..9905)
                     /locus_tag="AC16Y7_06360"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5709039.1"
                     /translation="MAVRPTLADLKLQRRSEDGDREALEHIARALEGRVLPGPGLVAA
                     GGEMDGQVAAGAAANDHTEVRASGSGSQTALALAPESIKILLEAMPAAIGLLQGEALI
                     YANTAFAYAFGYRSPSELMEAGGLGAILPGGTGELQAQDEVGRTGEIDALTHSRRRIK
                     VAFALSTLDKAADTVLLRLVDQADVEPEPPAPPIEEKTEPQDPAAPAAAAPPAPAEAE
                     PGPDADAATRRLDFLAKVSHEVRTPLNSVLGFAELMLQERFGPIGNLRYKGYAEDIHQ
                     SGLYSLSLLNDLLDISKIEAGKFELDFTAVDVGEVVEACVSSLQPLAKRARIVLRTSL
                     ADDLPVVVADPRRLRQILLNLLTNAIKFTKEGGQVIVSGTMVDGELRLRVRDSGVGMS
                     KNEIDYAMQPFHQLDTAPRRQSGTGLGLPLTKALADANRARLELSSEPGVGTSADVTF
                     PAERLFKG"
     gene            10206..10709
                     /locus_tag="AC16Y7_06365"
     CDS             10206..10709
                     /locus_tag="AC16Y7_06365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin"
                     /protein_id="HIR5709040.1"
                     /translation="MTETASIPQTPQEAAEQITETFKTAAKDFETMKFDTNVPESVRA
                     LAEKTVTQTRDAYERGKESLEESIDAVERSFDAAGQGATAYNRKLIDIAQRNLNSGFD
                     LAKSLASAKNLAEIVELQSAFIRSQFDVFASQAGEIRALTTKIAADTSEPIKEQVSRS
                     FEGMRKS"
     gene            10889..10965
                     /locus_tag="AC16Y7_06370"
     tRNA            10889..10965
                     /locus_tag="AC16Y7_06370"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10923..10925,aa:His,seq:gtg)
BASE COUNT         2101 a         3455 c         3555 g         2070 t
ORIGIN      
        1 gaagggcagg aatgagccag ccacgcaaga cacgcacggc gcgcaagaca cgtacggcgc
       61 gtagggcaca tcgcgctccc gtgcggccaa ccctcgtgcg gccaagtcca ccgctgccgc
      121 gaggacagcg cgcgcgcccg aacctggcgg gttcactgag cgcctccttt ccgagcttgg
      181 cctgatcgcg ggtgttatca ccacggtgat cttctacacg gtcggcaaag actgggccgt
      241 cgaattggat agcacagcct ggttggcgtt gatgttcgcc tggttgttcg gcgtgatgat
      301 ctggtgcgcc ttcggcgtcg tgcgtcatgc cgatgggctc gccgaaattc tcggcgagcc
      361 atacggcacg ctcatcctca ccatttccgt catcagcatg gaggtggcga tcctggccac
      421 ggtcatgctc agcggctcgc caaaccccac gctgccgcgg gagaccatgt ttgccatctt
      481 gatgatcgtg atgaacggca tggtggggac ggtgctggcg gtcggcggcc tgcggcacgt
      541 cgttcagcaa tacaatctgc aaggcgcgct ggcttacctt gccgtgatca cgccgctcgc
      601 cgtcctggcg ttggtgcttc ctaccttcac gaaatccacc tctggtccca cgctcgatac
      661 gcagcaggcg atctttttgg ggaccctaac ggcgctgctc tacgtcgcat ttcttaccat
      721 tcagaccacg cggcaccgca cgttcttcgt gcagccgagt cggaaggcag cgagcagcga
      781 gcggcccgcg ccggcaagtc ccgaacctca tcaccacggg cccgtttatt cggggcccta
      841 tcacgccgtg ctgctgatcg cgacgctgct gccggtcgtc ctgttatcca agccgttggc
      901 gagtatcctc gaccacggca tcgatcagct gggcctgccg acggcactcg gcggcgttct
      961 gatcgccgtg ctgatcctat cgccggaatg gtcggcggcg atcggcgccg cggtgcgcga
     1021 tcagttgcag cgcgccgtca atctcagcct cggcagcgcg ctctccacca tcggactgac
     1081 cgtgcctgtg gtgctggcca tcagtgtctt gacgggcacg cccctagaac tcggtctcaa
     1141 cgccgtcgat atggtgctgc tggcggtcac cctggttgtc tgcatgatca ccttttcagg
     1201 ggcgcccacg aacatcctcc tgggctttgt ccatctggtg ctcttcgcgg cctacctgat
     1261 gctgatcttc aagccttaga ccaagggccc accgttgtgg atatgcagcc atttccccgg
     1321 gggctgtggg gaacaaagca cggtagtgtg tcagtttgaa atctggaggt gacgctctca
     1381 ggcggtttca caggcgacta cctcatggag gatccgagcg gttacatctc gctcggcgtc
     1441 tatcgcgcgt tttgatgaag gtttggccca gagtgcactg ccggccccgc tctggcagtg
     1501 cttaagtccg cagcatgacg cgaggtcatg ccatactctg gttgtgcatt ctgtgtagag
     1561 cgatgttgcc agatgtcact gaagactcac tctggcacga agcatgacac gaagacgacg
     1621 gacagcgcaa acgggactta gcccttaccc cgtgtcttct tgcagctgtc aacgacgcgg
     1681 gcgaatctgt ctcggtgctc agcttaggaa acgactctac ggcgcggcct tatccgcctc
     1741 tttctcggcg gcttcctttg ccttgcgcgc ggcctcgcgg tcggtgcgcg ccgcgttgac
     1801 ccgcgccctc caggcggcga accgcttttg gagcgctgcg ccattgccgc gccaccaggc
     1861 cgcgtcgaac ttcagcgcct tttggaagtt ctgcggcgcc gtggatagga attccagcat
     1921 ctcgacattg atgacggggt gcttgccgac aagcggaaac gccgattttc gcatcggacc
     1981 ataggcagtg agctcggcct gatccgcaag tcgcgccggt acggtggcga agcggatgaa
     2041 acgcagcgcg tccttcttgt tgtcggagga tttggggatc gcccatgcat cgaagtcgta
     2101 gatttggccg tcccacagaa tgtcgatatt gcggtcgccg gcagctgcac ggaacaggcg
     2161 gccgctatag cttgcggcca tcaccacgtc gtcctccacg agccacgtaa tcgcctgttg
     2221 ggccttgttc cacaggcgga ccgcgcccgt aatcctgtcg agcgctgcga aggcacggtc
     2281 ggcgccctcg ggcgtggcga gttcgcggta gacattctcc ggcggaacac cgtcggccag
     2341 caaagcaagc tcgagcgaat agcgcgcatt gttgggaagc gcgcgtatgc cggggaagcc
     2401 ggccgtatcc agcagcgcgc tgatcgccgt gggggcgcct ttgggaaacg ctttgcggtc
     2461 gaaggcaata gccgtcgacc acgcgacgct cgcgaccccg caaggagaca acgctccggg
     2521 aaggaaatcc tccttggcgg cactcccgtc cgggcctgcg ccgagcgcgg aggcatcgat
     2581 cgtttctagc agaccctgct tgcatagcgt attgagcgcg tccgccgaaa cgtcgatcac
     2641 gtcgacgact gtgccgtcgt cgttgagcat ggtcttgagc ttcgcaagcc tgccgtcata
     2701 ggtcaccgtg tcgatcgaga cgccggtttc cttggcaaac ggctcgaaga tggcggcctc
     2761 ttgcgattgg ccgtaagccc cgccccatgt cgcaacgttc agcgtgttct gcgcatggcc
     2821 aagatttgcc gttgcgccga ggctcaaggc gactgtcacg gtgaacaagc cggcccgccc
     2881 gcctccggca gtggcagcct ctttcaacga tggacgatgg caatgacaga acacgggcgt
     2941 tgatctcaat cggctggtgc gttttgggtg ctcggcgcgg cggaaccact ttccgaactg
     3001 ggctgcgatg atgcatctgc gggagcaggg cggtctgcgc cgcgaacttc gggctgcgtg
     3061 agggggccac gaatggtgac tcgcggtggc gactccatga cgagttgtga ggcattcaat
     3121 ggtgtgacgg gattggcgat taagaagttc caatcgatgg tttgcttgcc gacatccacg
     3181 cctccggagc cggatattgt gccctgaggc gtctgcatgg tgaaggactg acaccaaaca
     3241 tggccggcag tgagctggca attggcatcg agcgtctcga atgcggtggt cgcatcctgg
     3301 ctccagcccg ccttgccctc cccagccccc gaggcgaggg cagaaaggtc gatcggtacc
     3361 gcgccgtcct ttgcggcaac ctcaaacttg ccgacaagat tgcggatgag ctcgtcccgc
     3421 gtctcgcctc ccgtcgacac gtcgaccttc agcacacccg tgcccttgat tggaacgtcg
     3481 agatcgaact gcgccaggca ggtctcgacg gcagcatccg agaggttgcc cgcaagcgat
     3541 gcctccggcc gcgcctccga caggttcaac gacacgcgcc ccgtcgccag accaccgcaa
     3601 agctcaagtg cgccgatctc gctagagatc gcgccgtctt ttgcggtgag ggtgaggcct
     3661 ccgcgcccaa gttccagcgt caaagcggcg atctcggccg ccgagagccg caagtccgca
     3721 tcgagatatc taagaatggc ccgatcgaac agcctgtcct ttgctgcctc actggtaccg
     3781 agatagggat cgagaacgag ccgctcgaag tcgagcgttc cctcgatccg cggccgatcg
     3841 cccgccgtga ccacgagaag gccaaccgcc tcattaccgt ccaaagagaa ggcgccacgg
     3901 tcgaacgtga gcgtcgtgcc gctccaatgc gccatgcctg agcccttcac atcatgcagg
     3961 ctttcgccct tggggagcga catccccagc cagttcagga cgtggcgcgc gtcgctcatc
     4021 tccgcttcca actcgccttc cgcctcaaga cgatcggcgg cgcgaacctt gcccgagaac
     4081 tcggcttttg ccccatccgc ggtcaccttc agcgtgacag gcgccagggt gccgtcctcg
     4141 gtctccgcaa ccgtaccggt atccatggcg aaggcgacgg tctcgccgcg atagtggaag
     4201 gacccaagcg cggcgagtgc cccacggcgt cccttgggat cgaggcggac ctcgaagtct
     4261 ttgacgattg ggacgccata agcgttcttg atcgtgccac cgcgcatacg caccgcgccg
     4321 aggggcgggt cgaccaaggc gccggagatc ggagaatcct ccggcgcggc cgcgtcggtt
     4381 tccttgagcg tgatgacagg tttgctgatc cgaagcgcgt cgatcttggt ccgaccgagc
     4441 aagaggtctg caaagtcgag agagaactcg acgtccggcg ccgtgatggc gccaatgcct
     4501 ggaagcttgg tggcgttggt gaggatgaac ccgccgcgca gcgagagcgg cggaaaatac
     4561 cggatgcgca gtggctccgt cagggtgatc gtgccgccag accattcgga gagaaccgcg
     4621 ccgatgcggt cgcgcagctg ggacgacgtg ccgatctggc gaggaagcgc cacggctccc
     4681 gcgatgagaa gcaccaagag gccaatgaga aaccatttcc tcggaagttg gcgaggcttc
     4741 atgacggcct ttctctgtcc gtttctgctg ggcgcttcca ggggtgggcc ttggggggag
     4801 gcgccggggg cactgctctt ttagccctaa gactccggct gggcgagccc aacgctcaca
     4861 ttttcctggc ttatcttgat attcgcaacg cccattcggc gtttcattcg accccgatca
     4921 tctttcccac agaggcccca gggcggctcc tggaaatcat gccatggagc agcagaaccg
     4981 gtgccaaact taccagtacg atggccaagg ccgacagccc cgcctcctcg tagcggtaga
     5041 gcgaggccag cgtgaagacc tgggtggcga gggtgtcgaa gttgaacggc cgcagcagca
     5101 gcgtcgccgg gagctccttc atggagtcca cgaacaccag catggccgcc gccccgagcg
     5161 cagggcgcag cagaggcagg tgcaccgacc acaacatttc gccgatcgag gcccccaagg
     5221 tccgtcccgc cgcgtcgata ttgcgggaga tcttgctcat gccggcctcc accgcgccga
     5281 gcgaggcggc caggaaacgg atcgtgtagg ccaggacgat ggcaaaggcg gttccggata
     5341 acagcagtcc cgtcgagacg ccgaacaatt cgcgcatcac cccatcgagg cggttatcga
     5401 gcccggcgag cggaatgagc agcccgatgg cgaggaccgt gcccggcacg gcatagctga
     5461 cggccggggc aagacttgcc gcgtggacga gcttggagcg cgtctgccgg cgggcgtagg
     5521 cgagaaccac ggcgaaggag acagcgagca cggccgcggc aacagacagg ccgaggctgt
     5581 gaatggcggc ctgccagaac gcgggcgaaa gtcctgagga gatatgcgca accgcatcgc
     5641 tcaccagaac ggctgcgggc agcacaaagc cgacgacaag aggcagagcg caagcgagcg
     5701 tcgcaagcca gcctttggca cccgtaagcg tgtcctcggg tagatcgcgt tacttgccgg
     5761 tcgtgttatg aaaccggcgg cccgaccgca aggcccgctc gagggcaagg aggacgaaga
     5821 cgaacgtcaa catcacgcaa gcgatctgtg ttgcgcctgc aagactgccg cgatcgagcc
     5881 acgtgtcgta gacggccacg gtcagcgtgt gcacgccgaa gaattccacg gcgccgatgt
     5941 cgttcaatgt ttccatcagc gcgagcgcga caccggccgc aagagcaggc cgggcgaggg
     6001 gcagggcgat ttgccaaaat gtttcggacg cgctgcgccc aagggtccgg ctcacttcga
     6061 tcacgcagat ggattgcgcg atgaagctcg cgcgcgcggt gatgtagacg taagggtaga
     6121 gcaccatgct cataaagaag atggcgccgc cgaggctcct aatgtcgggg aaccaatagt
     6181 cctgcgcgtt gcgccagccg aacgcctcgc gcatgcccgt ctgcaccagg cccgaatagt
     6241 cgaaaagttc caggtagcaa taggcgatga tgtaggtcgg catcgccagc ggcagcagca
     6301 ggagccactg gaaatagcgg cgcccgggaa agcggtacat ggtgacgagc caagcggtgc
     6361 cggtgccgat caggagcgtc agtaggccaa cgccgagcat cagacccaag gtgcgccgga
     6421 ccgcgccagg aagcacgttt gcgatgagat gcggccaggt atcgccgctc ggctggagcg
     6481 cgatcaccgc gatggcgatg atcggtgcaa gcgcgatggc ggagaatacg agcgcggcga
     6541 cggaccagcc gagattgccg ctctcagtca ggcgggcaag cttgcggcgg aacgaaacgg
     6601 tctctaaggg ccggctgtct gccgtgtgtt gctcagcgct ggccagcgag accgtcctcg
     6661 cggccggtgc gcgctgccat gagggcgctg accgcttccg gggagagacg ctgatcggcc
     6721 tcttgaagcg catcggcgaa ggcgttggcc gcgtcgatca ccggctcgac cagatcggag
     6781 ccggtcagcg ccagcgcaca agcctgcatg gccgggcagc gatgatgctg cgaggcggca
     6841 atcaacgaga tcgccatgca ctcgtcgttg cagaagccgg ggcgcgcgag cggatagtat
     6901 tgaatcttgc gggaggccga cgagcgcacg gcgcggaccc aacaggccag ctctgtgaca
     6961 gcgcgcttgg cgcgcgaagc accgagaacg cggttatatt cgttccagcc cttttcccaa
     7021 cagccgatgt cgccggtatc gtagccggca agccagcaac ggaagccgac gcccaccagc
     7081 cattcggctc ccgttcgagg cgagacgacg gcctgccggc cgttccgtgc atctatttct
     7141 ttgtgaatgc tcatagccgc cccctcatga gcttggacct tcgttaaagc cgactttgtc
     7201 aacgagttcg ctggccgtct tccgcagcgc ggcgatttcg ttgagcgaga tggggtcgga
     7261 cttgaatgtg ccccaagatt gtacgagccc cgaccaagga acgccgtcct tgacgggata
     7321 ctcgtggttc acttccgcgt acatttcttg accgtcgtcg gaagccagaa attcgacaag
     7381 cttgatcgcg ttttctttgt tgggcgcgtt cttcgccagg accgcaccgg acaggttgac
     7441 gtgcgtgccg cgatccttgg tgttggggaa caggatgttc accgcgttcg cccactcttt
     7501 ctgctccgga tgtttctcgt ccgtgagcat cttgcccatg taataggtgt tgccgatggc
     7561 gagatcgcat tcgccggcat agacgccctt cacctgaagg cgatcgttgc ccgcgggctt
     7621 gcgcgctaag ttggacttca cgccgcgcag ccactcctcg gtccagtcct cgccgtgcgc
     7681 ggcgatcatc gaggcgatca gcgcgatgtt gtaagggtgc tggcccgaac ggatgcaaat
     7741 cttgccgcgc catttcggct cggccagttc ctcgtaagta atggtgtctt gcgtcacgcg
     7801 gtccttggac gcgtagacca cgcggccccg gcgcgtcagt ccgacccaag cactatcctc
     7861 gtcgcgatag gctgccggga tcgccgccgt gttcgtctca gacgtgatgg gttgggcaat
     7921 gcccgacgcc gcagcctggc tgagattgcc gatatcggtg gtgagcagca catcggcggg
     7981 gctgtttcgc ccctcggcct ggatgcgctc tatcagtccc ttcgaagcga agatgatgtt
     8041 ggtcttgatg cctgtctgcg cagtgaaggc cttcagcagc ggctcaatca gatagggctg
     8101 ccggtacgaa tagatgttca cctcgccggg attcccagcg cgggcgatag agggcgcaaa
     8161 agcaaggctg agagttgcga gcgccgcggc gcacgccagt gtccaacgca tcaatagcct
     8221 ccaatggccg gggttcaagg ccccggtatc ggactgaaaa aaccgcgctc tgccctaagg
     8281 ctagagggtc attatttcgc ttaaggcttg ggcttggcca ctgtcaacaa gattatcgaa
     8341 atggaactca acttagaagc cttctaatgc gtgaagttag attagaaaca ttcaacgtca
     8401 caagtattat tccgcgagac gacagacgaa agtcagaaaa tcactccaag aggtctgtat
     8461 tgccccagtg aactcgcagt tgcgaaatgg atttcgtgcg ggatcaagac cggcggcgcg
     8521 gggtgcgatc agcccttaaa taaccgctcg gcggggaagg tcacgtctgc ggacgtgccc
     8581 actccgggct cactggaaag ttcgagccgc gcgcggttcg catcggccag cgccttggtc
     8641 agcggcaggc ccagacccgt gcccgactgg cggcgcggcg ccgtgtcgag ctggtggaag
     8701 ggctgcatgg cgtagtcgat ctcgttcttg ctcatgccga cgccgctgtc gcgcacacga
     8761 aggcgcagct ctccgtccac catcgtaccg gacacgatca cctgaccgcc ttccttggtg
     8821 aacttgatcg cgttggtgag aaggttgagc agaatctgcc gcaggcgcct cgggtcggcc
     8881 acgacgacgg gaaggtcgtc cgccagcgat gtccgcagca cgatgcgcgc gcgttttgcc
     8941 agcggctgca ggctggacac gcacgcctcg acgacttcgc cgacgtcgac ggcggtaaag
     9001 tcgagctcga acttgcctgc ctcgatcttg gagatatcca gaaggtcgtt gagcaggctg
     9061 agtgagtaga gcccgctttg atggatgtcc tccgcgtagc ccttgtagcg caaattgccg
     9121 atcggcccga aacgctcctg gagcatcaac tcggcaaagc ccaggacaga attgaggggc
     9181 gtgcgaacct cgtggcttac cttggcgaga aaatcgagcc ggcgtgtcgc ggcgtcggca
     9241 tcgggaccag gctccgcttc ggccggggca ggcggggcgg cggcggccgg ggcggcaggg
     9301 tcttgtggct cggtcttttc ttcgattgga ggtgccggtg gttcgggctc cacatccgct
     9361 tggtcgacca gccgcagcag cactgtgtcc gctgccttat cgagtgtgga gagggcgaag
     9421 gcgaccttga tgcgccggcg ggagtgcgtc agcgcatcga tctcgcctgt gcgtccaacc
     9481 tcgtcttggg cctggagctc gcccgtgccg cccggcaaaa tcgcgccgag cccacccgcc
     9541 tccatcagct ccgagggaga gcggtagccg aatgcataag cgaacgcggt attggcatag
     9601 atgagcgcct cgccctggag gaggccgatc gcggccggca tggcctcgag tagaattttg
     9661 atgctctcgg gtgcgagcgc caaggccgtc tgtgagccgc tcccggacgc ccggacctcg
     9721 gtatggtcat tggcggcggc gccggcagcg acctgaccgt ccatctcgcc cccggccgca
     9781 accaagccag ggccaggaag cacgcggccc tcgagcgcac gcgcaatatg ctccagagcc
     9841 tcgcggtccc cgtcttcgct acgccgctgt agtttgagat cggccaaggt cggcctcact
     9901 gccatacaaa atgagcccgt ccctgtgctg gcgcacgccg gacgcgctcg gccacgtgat
     9961 cgcgcccctt ggggcgcctg attgattcaa ccactaaatt taggcgaatt cgctccgttg
    10021 catagggcga atgcgcgtat tcgtccacag gccaaaaaag atgcgccttt gggcactccg
    10081 cctcttgtgc caccgcactg cacaatatat attgcactgc aacaaagagg gatgcggagg
    10141 catcgtgcca accgcattcg ttcccgcgtg aggcgagaca agtagattcg cgaagaggtg
    10201 caatcatgac tgaaactgcc tccattcccc agactcccca agaagctgcc gagcagatca
    10261 ccgagacctt caagaccgcc gcgaaggact tcgagacgat gaagttcgac accaatgttc
    10321 cggagagcgt ccgtgctcta gccgaaaaga cggtgacgca gactcgcgac gcctatgagc
    10381 gtggcaagga gtctctggaa gagtcgatcg atgctgtcga gcggtcgttc gatgctgctg
    10441 gtcaaggcgc gacggcctac aaccgcaaac tcatcgatat tgctcagcgc aatctcaatt
    10501 ccggtttcga cctggccaaa agcctcgcta gcgccaagaa cttggccgaa atcgtcgagc
    10561 tccagtcagc tttcatccgc agccagttcg acgtgtttgc gagccaggcg ggagagattc
    10621 gggctctgac caccaagatc gccgccgaca ccagcgagcc gatcaaggaa caggtttcgc
    10681 gttccttcga aggcatgcgc aagtcgtaaa cggcgatgcc gtggcagcgt ttgtagcgta
    10741 gagtagcgta aatgcgtaac tgttaaggcc cggggccatg gcccgggcct ttttatttgc
    10801 gtgaatttct gccgtttcgg gcggcttgca agaggtcggg cctgccctta ggttccggag
    10861 cctgaggccc aagtgatgct ggctaagtgc cgctgtagct cagttggtta gagcgcttga
    10921 ttgtggatca agaggtcact ggttcgaatc cagtcagcgg taccaatatc caacagtttt
    10981 agggtgatgc agccccgata cgcttgcagc tcgaccgggg tatcatcgac cagcgctggg
    11041 ggccgttggc gctgtacacg gtcaggccgg ccacacgatc atccggacag ggagcggaat
    11101 gaagctgaca accttcatcg acaggcaatg gatgcgcttc aaccccgaca agcgggcgcg
    11161 catccacgag gcctgcaagg g
//
LOCUS       DBNHVU010000157         4954 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_2062787, whole genome shotgun sequence.
ACCESSION   DBNHVU010000157 DBNHVU010000000
VERSION     DBNHVU010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_2062787"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="AC16Y7_06375"
     CDS             <1..680
                     /locus_tag="AC16Y7_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HIR5709041.1"
                     /translation="SPDVQEQLRQFGVPDDAYDATVTSGDLTRHELGKYTGARVFHLG
                     PERDRPIFAGLDVVLAGPEEADLVVCSGLFDDDTETPDDYADMLRTLAARGLPMICAN
                     PDHMVERGNRLVYCAGALAAVYETFGGKVVYAGKPHVPVYELALETIAQLSGEAVGRE
                     RVLAIGDGVNTDIAGAAALGIDAVFVASALHAPGEGGQALDAPHLNELFAQTVRRPIA
                     AMRGLNW"
     gene            complement(683..1177)
                     /locus_tag="AC16Y7_06380"
     CDS             complement(683..1177)
                     /locus_tag="AC16Y7_06380"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HIR5709042.1"
                     /translation="MDQTDRPMVAGTPQGRYKMACTCGADSVSEEVVALTNTLRELAA
                     MRSTAEDAATRILDSAEGLLAMAGKNEAEKAEEAILSIMTACGFHDIVGQRVTKITET
                     LDQVIAASHGEVAAKARKPNASNSVSAKSEPGIALEGPALPGASKDRAQDQAGVDALF
                     SDKR"
     gene            complement(1215..1571)
                     /locus_tag="AC16Y7_06385"
     CDS             complement(1215..1571)
                     /locus_tag="AC16Y7_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5709043.1"
                     /translation="MAFDPGMPVLVVDDMKQLGFADVDEANDGNAALGMMRETDYGLV
                     ISDWNMEPMTGFELLREIRADNHLSRTPFIMVTAESKTDNVVTAKKAGVNNYIVKPFN
                     AATLKSKIDAVFSAAA"
     gene            1799..2248
                     /locus_tag="AC16Y7_06390"
     CDS             1799..2248
                     /locus_tag="AC16Y7_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR5709044.1"
                     /translation="MTSTASLGHGYYLEDLSPDMEASVSKTITDEDVRAFAKLSGDIN
                     PVHIDDDFAAGTMFKKRIAHGFLTGALFSTVLGTKLPGPGCIYMSQTMKFKGPVYIGD
                     EVVATCKITAVDTEKARVALACDCSVNGKTVLAGEALMMVDRKAAGQ"
     gene            2269..3282
                     /locus_tag="AC16Y7_06395"
     CDS             2269..3282
                     /locus_tag="AC16Y7_06395"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR5709045.1"
                     /translation="MDIVRSWRDTPPALKGAVLAIGNFDGVHRGHQAVLNEVRRIASA
                     EGRRAGAVIFEPHPREFFAPDQPFFRLTPLPVKLELLEALDLDQTFVLSFDKAMSSLS
                     ADDFANEVVGEGFGASHVVVGYDFVYGKGRTGSVDHLALIGKTRGFGVDVVSPVTLAG
                     GVTFSSSTIREHLRRGEVREAAEQLGYWWRARGRVGKGAGRGKDLGFPTLNFKLAPGQ
                     DVCHGIYAMRVHHAGERHHAAGYVGPRPTFGAGDEPVLEAYLLDFSGDLYDHEVEVEF
                     IDFVRPDHTFESAEALAAQMADDCSQVQEILAAVDADDPMRRFTLGDVLADRALDCGE
                     AGC"
     gene            3327..>4954
                     /locus_tag="AC16Y7_06400"
     CDS             3327..>4954
                     /locus_tag="AC16Y7_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR5709046.1"
                     /translation="MAKTTKSGKPAARRKVARKAKRTKTAGSPKAAAKRKTAAKRKVA
                     TKRKAAPKRKAAAKRATAKKATAKRRPTVKKRVASKKKRTTRKSTTRKAAPKRKVTAK
                     RKATKAKPKKTVTKRRPTSKAKARKPANKRVAPKKRAAQRTRTVSPKKAAAKKPVARK
                     AAPKPKATPKPAVKRVQPQVERDWSETLFLPKTDFPMKAGLPRREPELLERWAKMGLY
                     DRLREEAMSRPKFVLHDGPPYANGRIHIGTALNKILKDAVTRSHAMMGKDANYVPGWD
                     CHGLPIEWKVEEENYRAKGRAKPDLSDTDALIAFRRECRDEAAKWLDVQREEFKRLGV
                     EGDWDHPYTTMSYEAEATIARELTKFAMNGLLYRGSKPVMWSVVERTALAEAEVEYQE
                     IESPTIYVKFPVMFASEDSQDPQALQHASVVIWTTTPWTIPGNRAISYSPSISYGLYE
                     VTQAPADNWANVGERLILADALAETVKEAARIDGWSRQGDVTAEMLRQSMCAHPLRGQ
                     SDYRFMVPLLSGEHVTEETGTGFVHTAPGHGVDD"
BASE COUNT          963 a         1564 c         1609 g          818 t
ORIGIN      
        1 cgagccccga cgtgcaagag cagctccgcc agttcggcgt gccggacgat gcctatgacg
       61 cgaccgttac gtcgggcgac ctgacccgcc atgaactcgg caagtacacg ggcgcgcgcg
      121 tcttccatct cgggcccgaa cgcgaccggc cgatcttcgc cgggctcgat gtcgtgctcg
      181 ccgggcctga ggaggccgat ctcgtggtgt gctccggcct gttcgacgac gacaccgaaa
      241 cacccgacga ctatgcggac atgctgcgca ccctggcagc gcgcggactg cccatgatct
      301 gcgccaaccc cgatcacatg gtggaacgcg gcaatcgcct cgtttactgc gccggtgcac
      361 tcgcggcagt ctatgagacg ttcggcggca aggtcgttta tgccggtaag ccgcatgtgc
      421 ccgtctacga gctcgcattg gagacgatcg cgcagctaag cggggaggct gtcggcaggg
      481 agcgggtcct tgccatcggc gatggcgtca acacggacat cgccggcgcc gccgcgcttg
      541 ggatcgatgc ggtgttcgtg gcaagtgcgc tgcatgcgcc gggcgaggga ggccaagcgc
      601 tcgatgcgcc ccatctcaat gagctgttcg cgcagacagt gcggcggccc atcgccgcga
      661 tgcgcggcct caactggtag cgctagcgtt tgtcgctaaa gagagcgtcg accccggcct
      721 gatcttgggc ccgatcttta ctcgcgcccg gcagggcagg gccctcgagg gcgatgcccg
      781 gttcggactt ggccgagacc gagttggagg cgttgggctt gcgcgctttt gcggccacct
      841 cgccgtgact ggcggcgatg acttggtcca gcgtctccgt gattttggtc acgcgctggc
      901 cgacgatatc gtggaagccg caggccgtca tgatcgacag aatggcttct tccgccttct
      961 ccgcttcatt cttgccggcc atggccagga gaccttcggc ggaatcgagg atgcgcgtgg
     1021 cggcatcctc agcggtgctg cgcatagcgg cgagctcgcg cagcgtgttc gttagggcga
     1081 cgacctcctc cgaaacggaa tcggcgccgc aagtgcatgc catcttgtag cggccttgcg
     1141 gcgtgcccgc gaccatgggc ctgtcagtct ggtccatgaa agtccttttg cggaattgag
     1201 agtcgggaat ctgcctaagc ggccgcgctg aaaacggcgt cgatcttcga cttgagcgtt
     1261 gcggcgttga acggcttgac gatgtagtta ttcacaccgg cctttttcgc tgtgaccaca
     1321 ttgtcggtct tcgactctgc cgtgaccatg atgaaaggcg tgcgcgacag gtgattgtcg
     1381 gcgcgaattt cgcgcagcaa ctcgaagccc gtcatcggct ccatattcca atccgaaatg
     1441 acgaggccat agtccgtctc gcgcatcatg ccgagcgcgg cattgccgtc attcgcctcg
     1501 tcgacatcgg cgaaaccaag ctgcttcata tcgtcgacga cgagcaccgg catgcctgga
     1561 tcgaaagcca tttcgctaat cctccgccag acaagagcct ggtttggcgg cccaaaggag
     1621 accttctgcg ggtgaaaaat caggcttaga ccctaacgag tggccgtgaa cggggggtta
     1681 agcacggcat ttttagacgt gtgcggatga attggctggg gcgccttgtt gcggcgcagc
     1741 aatcccttcg ttattttgcc cagcttcaac aacccgacaa atcaagcagg gaacacatat
     1801 gacgagcacc gcgagcctgg gtcacggcta ttatttggag gacctctccc ccgatatgga
     1861 ggcgagcgtc tccaagacca ttaccgacga ggacgtgagg gccttcgcca aactgtccgg
     1921 cgacatcaat ccggttcata tcgatgatga tttcgcggcc ggcaccatgt tcaagaagcg
     1981 catcgcccac ggctttctga cgggcgccct gttctccacg gtgcttggga ccaagctgcc
     2041 cgggcctggc tgcatctata tgagccagac catgaagttt aaggggccgg tctatattgg
     2101 cgacgaggtt gtggcgacct gcaagattac cgccgtcgac accgaaaagg cgcgtgtggc
     2161 gctcgcctgc gactgctcgg tgaacggcaa gaccgtgctc gcaggcgagg cgctcatgat
     2221 ggtcgaccgc aaagcggcag gccaatagcg ccaatcgaag gagttccgat ggacatcgtc
     2281 cgctcctggc gggacacgcc gccggctctg aagggcgcgg ttttggcgat cggcaatttc
     2341 gacggtgtcc atcgcggcca tcaagccgtg ctcaacgagg tgaggcggat cgcgtccgcc
     2401 gaaggccgcc gtgccggcgc ggtcatcttc gagccgcatc cgcgcgagtt cttcgcaccc
     2461 gaccagccct tcttccggct gacgccgctg ccggtgaagc tcgaattgct ggaggcgctg
     2521 gatctcgacc agaccttcgt gctgagcttc gacaaggcga tgtcgtcgct ctccgccgac
     2581 gacttcgcca acgaggtagt gggtgagggc ttcggcgcca gccacgtcgt ggtgggctac
     2641 gacttcgtct acggcaaagg gcgcaccggc tccgtcgatc acctggcctt gattggcaaa
     2701 acgagagggt ttggcgtgga cgtggtctca cccgtcacct tggcgggtgg cgtcaccttc
     2761 tcgtcgagca cgatccgcga gcacttgcgg cgcggcgagg tgcgcgaggc ggccgagcag
     2821 cttggctatt ggtggcgcgc gcgggggcgc gtgggcaagg gcgctggccg cggcaaggac
     2881 ctcggcttcc cgacgctcaa cttcaagctg gcgccggggc aggacgtttg ccacgggatt
     2941 tacgccatgc gcgtgcacca cgccggggag cgccatcacg ccgcaggcta tgtgggcccg
     3001 cgtcccacct ttggcgcggg cgatgagccg gtactggagg cctatctcct cgacttctcg
     3061 ggcgatctct acgaccatga ggtcgaggtc gaattcatcg attttgtgag gcctgaccac
     3121 accttcgaaa gtgccgaggc gctggcggcc caaatggccg acgattgcag ccaggtccag
     3181 gagatcctgg ccgcggtcga cgcggacgat cccatgcggc gtttcacgct tggggacgtg
     3241 ctggccgacc gggcactaga ttgtggcgag gccggttgct agaacgcctc gcacatcgaa
     3301 tcgaggctcg ggcggacaag aaaaccatgg caaagacaac gaagagcggc aaaccggccg
     3361 ccagacgcaa ggtcgcgcga aaagcaaagc gcacgaagac ggctggatcg ccgaaagcgg
     3421 ccgcgaagcg gaagaccgcg gccaaacgca aagtggcaac taagcggaag gcggcaccaa
     3481 agcgtaaagc cgccgccaaa agggccacgg ccaagaaggc tacggccaag cggcgcccca
     3541 cagtcaaaaa gcgcgtcgcg tccaaaaaga agcgcacgac aaggaagtcc acgacgcgca
     3601 aagccgctcc gaagcgcaaa gtcacggcca agcgcaaggc caccaaagcc aaaccaaaga
     3661 agacggtcac caagcggcgc ccaacgtcca aggcgaaggc ccgcaagccg gccaacaagc
     3721 gcgttgcacc caagaagcgc gccgcgcaaa ggacgcgcac tgtgagcccc aagaaggcgg
     3781 cggcaaagaa gcccgtggcc cggaaagctg caccgaagcc caaagccaca ccgaagcccg
     3841 cagtgaagcg cgttcagccg caagtggagc gggactggag cgagacgctg ttcctgccca
     3901 agaccgactt ccccatgaag gcgggcttgc cacgccgcga gccggagctg ctcgagcgtt
     3961 gggccaagat gggcctctac gaccgcttgc gcgaggaggc gatgagccgc ccgaaattcg
     4021 tgctgcatga cgggccgcct tacgccaatg gccgcatcca tatcggcacc gccctcaaca
     4081 agatcctcaa agacgccgtc acccgttcgc atgccatgat gggcaaggac gcgaactacg
     4141 tgccgggctg ggactgccat ggcctgccca tcgagtggaa ggtcgaggag gagaactacc
     4201 gcgccaaggg ccgcgccaag ccggacctga gcgataccga tgcgctgatc gcgttccggc
     4261 gcgaatgccg cgacgaggcc gccaaatggc tcgacgtgca gcgcgaggag ttcaagcggc
     4321 tcggcgtcga gggcgattgg gaccacccct acaccacgat gagctacgag gcggaggcga
     4381 cgatcgcccg cgaactgaca aagttcgcca tgaacggcct gctctatcgc ggctccaagc
     4441 ccgtgatgtg gtccgtggtg gagcgcaccg cgctggccga agccgaggtc gagtatcagg
     4501 agatcgagag cccgacgatc tacgtgaagt tcccggtgat gttcgccagc gaggacagcc
     4561 aggacccgca ggcgctgcaa cacgcctccg tggtcatctg gaccaccacg ccctggacca
     4621 ttccaggcaa ccgcgccatc agctattcgc cgtccatctc atacgggctc tatgaggtca
     4681 cccaggcgcc ggcagacaat tgggccaatg tcggcgagcg attgatcctc gccgatgcgc
     4741 ttgccgagac agtcaaggaa gccgcgcgga tcgacggctg gtcgcggcaa ggcgacgtca
     4801 cggccgagat gctgcgccaa tccatgtgcg cgcatccttt gcgcgggcag agcgactacc
     4861 ggttcatggt gccgctcctg tccggcgagc acgtcaccga ggagacgggc accggcttcg
     4921 tgcacaccgc accgggccac ggcgtggacg actt
//
LOCUS       DBNHVU010000158         6739 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1098030, whole genome shotgun sequence.
ACCESSION   DBNHVU010000158 DBNHVU010000000
VERSION     DBNHVU010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6739)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6739)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6739
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1098030"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(345..1025)
                     /locus_tag="AC16Y7_06405"
     CDS             complement(345..1025)
                     /locus_tag="AC16Y7_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5709047.1"
                     /translation="MNVLLVEDEPRVSDFIDRGLRAENHGVTVAPTGGEGVDLAKTGE
                     FDVIVLDLMLPDMHGYDVCQRLRQDGNHTPILMLTAMDALEDKIKGIKIGADDYLTKP
                     FDFDELLVRIEALARRAHQFAQSSNVLKVADLELDRELLEVKRGGEPIKLTAKELAIV
                     ELLMGAPGRVFSRTRILNQVWGYSEDPLTNVVDVYIARLRRKIDTKGREALIETVRGH
                     GYRLKAAS"
     gene            complement(1022..2617)
                     /locus_tag="AC16Y7_06410"
     CDS             complement(1022..2617)
                     /locus_tag="AC16Y7_06410"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5709048.1"
                     /translation="MRYRTKLLIAAGLLLAMSLSAAALAYWGVAQSHRYLTRSRIAHE
                     QLERQLQLSRHSQQLFKAWTDTLLTGMTDRPLGAAYLNKVIESDLSALQDLSEKELTI
                     AGSEEHRAESAEIERLADIRREYQHILDQLAEVEQLRSRGRPDVAWQNLIELLRGGLD
                     QKLNELIEVAVADETAEVLRIDADAAQLLSRLERISQIHAALAILTTLGLLILLVRGL
                     RAPLADLLAGTRKLAEGDLSYRIAVTGRDEFAELGRGFNGMAADLQAHQKALEDAHAN
                     LESLVEERTEELSKANESLRQIDETRRAFFADISHELRTPLTVIRGEGEISLRGKNKR
                     VAEYKHSMERIVEQAKHLSVLVNDLLFIARQGAGAAKLKLKPIDLNALIEKVCGDAKI
                     IAHDKAVGITVANGTAHETVVGDPARLRQLFLVLLDNAVRYSQSKGAIEVGIDRSDGK
                     VTVSVSDSGIGIAPEELEGIFERFRRGGNATEMNEEGLGLGLPVAKAIVEAHKGTIEM
                     RSKVGEGTTVLVTLPAETEGGQA"
     gene            2984..3733
                     /locus_tag="AC16Y7_06415"
     CDS             2984..3733
                     /locus_tag="AC16Y7_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709049.1"
                     /translation="MLLATAALTLVSAPRQAWAEFEIPEVDAEKGAIEAEYRGAQHWG
                     LPEADEDGDIDALRQSHEVEFQYGLTDWWMLRLTPNVEKPAGGSAELSSIGIETQFVF
                     VTRNDGTFGLALMIGYGPYSLHIDEEDEFEFGPVIELAPGRWLFTLNPRLADEPGNAD
                     HEGLGFEYAAQARYSVTDRWGVAAMGFGVIEELVNAGPFDGQAHVLGPSLYVFSAPDA
                     EREWTLGAGVLFGLTESSPDTALRITFAVEY"
     gene            3736..4608
                     /locus_tag="AC16Y7_06420"
     CDS             3736..4608
                     /locus_tag="AC16Y7_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709050.1"
                     /translation="MLRTTHRRLRLLRVASAATIAIAAATPAFAEFEIQESTIDPGEI
                     QLQYRGAWHSGVPAADLDDDVDLGLIIDEPEALLLQSHEFEFQMSVTSRWLLALTHAF
                     AEPVGDHFQLAAIEFESQIEFIEREGDGVGLAMQGGMEKPVQEASEKFDPDFHFGPIV
                     ELSKSNFTLVTNPLFFKLIGDLNRQEGWGFEYGWQGRYEAFGGRLWLAVEMFGEIEDM
                     SNAGPFDQQLHSLGPTFYYTFGKDDDEEGAGDEDEDIYQKSRELTVSLGAQFGLTKEA
                     SDVALKVFIGYEFN"
     gene            complement(4627..5400)
                     /locus_tag="AC16Y7_06425"
     CDS             complement(4627..5400)
                     /locus_tag="AC16Y7_06425"
                     /inference="COORDINATES: protein motif:HMM:NF024503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB family protein"
                     /protein_id="HIR5709051.1"
                     /translation="MAIRIITWLISLGVHGAFALAMLMPASGGVALEEGTGDDIMVVE
                     QGVALQGFAKLGEDVISVDAVEAPPMMMAAAQPLEQVEAVEEQEELPVEEVSEIEPVE
                     DEVIASEAGPESELIEPVQEELKEPEPEVKEQTLPQQMPTIPQETVIAMRESTGEEKK
                     GGDATAHRQYLGQLRTHLEKAKVNPRTTLIGTAVVRLTVKSDGVLVAQRIVKSSGSKV
                     LDNAAIDSIKKAAPFPPIPSAVGKDTVEVSVPFKFSVRR"
     gene            complement(5422..5877)
                     /locus_tag="AC16Y7_06430"
     CDS             complement(5422..5877)
                     /locus_tag="AC16Y7_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR5709052.1"
                     /translation="MGMSLGNNGGGEDAEYRPISEINITPMVDVMLVLLIIFMVTAPL
                     LVAGVPVDLPSTSAAKVSQPKKPMIVSLASDGSIYVREEQVTEDTLVPTLIALRQAEG
                     DTVVYVRGDKSRPYGEVMDILGRVGQSGYARVSLLSQPKANGGAAGQSQ"
     gene            complement(5877..6590)
                     /locus_tag="AC16Y7_06435"
     CDS             complement(5877..6590)
                     /locus_tag="AC16Y7_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR5709053.1"
                     /translation="MEEEITVTAATTHDYSVWGLVMQADPVVKGVMILLVLMSIACWA
                     IIFEKLVRIGWLNGAIRRLAQSRDSRARGGLSRTVLEAGERELDADLDDTPTERRYRA
                     ENAMRFSLKSQIQKLEVGLPFLATIGSAAPFIGLFGTVWGIVNSFTAIATQKDTSLAV
                     VAPGIAEALIATALGLAAAIPAVVAYNQITVALGRSFARGNSAVIDIARDLVAGETEQ
                     KPQGRRGTNVEPLRAGKRS"
BASE COUNT         1218 a         2035 c         2113 g         1373 t
ORIGIN      
        1 acgccgatat cgaccgtggt cggatcttcc gcatgcgcgg gcgtgccgct catgagaacc
       61 aggaataaga aagctcacag acgtgaccgc aacggggctc ctccacgtgg gctattggtt
      121 caatctgact tctctggggt gtgccaggcg cgggtgggcc cgaggccctc gttgccggac
      181 aggcgcggcc ccgcgcccct ctggcaaggc ccggaaaaag gtatggcgcg tcataggcaa
      241 gagtggcgag gcacaatcta ccccatgcgc cggacttggc aaggttccaa tgcctcgctg
      301 ccagtgctcg gattggccta tgggaaggat gtcgcaagtc cgcatcagct tgccgccttc
      361 agccgatagc cgtgacctcg gaccgtctcg atgagagctt cgcggccctt ggtatcgatc
      421 ttgcgccgga gccgcgcgat atagacgtcc accacgttgg tcagggggtc ttcgctatag
      481 ccccagacct ggttgaggat ccgggtgcgc gaaaataccc gcccgggggc gcccatcagc
      541 agctcgacga tggcaagttc cttggccgtg agcttgatcg gctcgccacc gcgtttcacc
      601 tccagcaact ctcggtcgag ttcgagatcc gccactttca acacattgga gctttgtgcg
      661 aactgatggg cgcggcgcgc caacgcttcg atgcggacga gaagctcgtc gaagtcgaac
      721 ggcttggtca ggtagtcgtc ggcgccgatc ttgatgccct tgatcttgtc ttccagggcg
      781 tccattgccg ttagcataag gatcggcgtg tggttcccgt cctggcgcaa gcgttggcaa
      841 acatcgtagc catgcatgtc gggcagcatg aggtcgagca cgatcacgtc gaactcgccc
      901 gtcttggcca aatcgacgcc ttcaccgccc gtcggggcga cagtcacccc gtggttctcc
      961 gcgcgtaagc cgcggtcgat gaagtcggac acacgcggct cgtcttccac caagagcacg
     1021 ttcacgcctg ccctccttcc gtttcggcgg gcagggtcac caagacggtg gttccttcgc
     1081 cgaccttgct tcgcatctca atcgttccct tgtgggcctc aacgatggcc ttggcgaccg
     1141 gcagtccaag gccaaggccc tcctcgttca tctcggtcgc attgccgcca cggcggaacc
     1201 gctcaaagat cccctcaagc tcctctggcg cgatacctat gcccgaatcg gaaacgctga
     1261 ccgtgacctt gccgtcggag cggtcgatcc caacctcgat ggcgcctttg gattgtgagt
     1321 agcggaccgc gttgtcgagc aaaaccaaaa agagctgccg cagccgtgcg gggtcgccga
     1381 cgaccgtctc atgggccgtt ccattggcga ccgtgatgcc gaccgccttg tcgtgagcga
     1441 tgatcttggc gtcgccgcac accttctcga tcaacgcgtt gaggtcgatg ggcttcagct
     1501 tgagcttggc ggcaccggcg ccctgacggg cgatgaacag gaggtcgttg acgagcacgg
     1561 aaaggtgttt ggcctgttcg acgatgcgtt ccatggagtg cttgtactcg gcaacgcgct
     1621 tgttcttgcc ccggagcgag atctctccct cgccacggat gacggtgagg ggtgtgcgga
     1681 gttcgtgact gatatcggcg aagaaggcgc ggcgcgtctc gtcgatctgg cgtagagact
     1741 cgttggcctt gctcaactct tcggtgcgct cttcgacgag cgactccaga ttggcgtggg
     1801 cgtcttcaag cgccttttgg tgcgcttgaa gatcggcggc catgccgttg aagccccggc
     1861 caagctcggc aaactcatcg cgtcccgtta cggcaatgcg ataggacagg tctccttcgg
     1921 ccagctttct cgtgccggcg aggaggtcgg cgaggggtgc gcgcaatccc cgcaccagga
     1981 ggatcaacaa gcccagcgtg gtcaggatgg cgagggcggc atggatctgg ctgatgcgtt
     2041 ccagccggct caggagctgg gccgcgtcgg catcgatcct caagacttcc gcagtctcgt
     2101 cggccacggc aacctcgatc agctcgttga gcttctggtc gagcccgccc cgcaggagtt
     2161 cgatcagatt ctgccaggcg acgtcgggac ggccccggct gcggagctgc tcgacttcgg
     2221 caagctgatc gaggatgtgc tgatactcgc gccggatgtc ggccaggcgc tcgatctccg
     2281 ccgactccgc cctgtgctcc tcactgccgg caattgtcag ctccttctcg gagaggtctt
     2341 ggagcgccga gagatcggac tcgatgacct tgttgagata agcagcgccg agagggcggt
     2401 cggtcatgcc cgtgagcaag gtgtcggtcc aggccttgaa cagctgctgg ctgtgccgtg
     2461 aaagctgaag ctgccgctcc agctgctcgt gagcgatacg tgagcgggtg aggtagcggt
     2521 gcgactgggc cacgccccaa taggcgagag ccgctgccga gagcgacatt gccaggagca
     2581 agccggccgc aatcagaagt ttcgtgcggt accgcatccg tcttcacgtc tccttggtcc
     2641 cggggagggg tggcaaagcc cgctcttttt caaacggcaa tcctccgcca cccgccggac
     2701 ggcctgatac ccatactgca ttttctcaga aaatgagggg ggttgggggg tgcaggcgcc
     2761 caacttgtcg tgaatattct gccgatcttc tcatagaatt tgcaattctt tcccgcatct
     2821 cttgctagac acgccgtacc ccagcgttgg atatttcccc cctggcagtt gggatcgccg
     2881 aaaaagcggg caaaccgctc ggaacttgag ttgttggggg ggcgggtttt tgcatccggt
     2941 ttggaggcgc gcgggcatat tccgcaggat cggcttgggg gctgtgctcc tggcgacggc
     3001 tgcgctgacg ctcgtcagcg cgcctcgaca ggcttgggcc gaattcgaga ttcccgaggt
     3061 tgatgccgaa aagggcgcga tcgaagccga gtatcggggt gcgcagcact gggggctgcc
     3121 ggaggcggac gaggacggcg atatcgatgc gctccgccag agccacgagg tcgagtttca
     3181 gtatgggctg accgactggt ggatgctgcg cctcaccccc aatgtcgaga agccggcggg
     3241 cgggtccgcc gagctctcat ccatcggtat tgaaacacag ttcgtgtttg tcacccgcaa
     3301 tgacggcacg ttcggcttgg ccttgatgat cggctatggg ccgtactctc tccatatcga
     3361 cgaggaggac gagttcgaat tcggtccggt catagagttg gcgccggggc gatggttgtt
     3421 caccttgaac ccgcgactcg ccgacgaacc ggggaacgcc gatcacgaag gcctcggctt
     3481 tgagtatgcc gcgcaagcac gctacagcgt gacggaccgc tggggcgttg ccgcaatggg
     3541 cttcggtgtg atcgaggaac tggtgaatgc ggggccattt gacggccagg cccatgtgct
     3601 cggccctagc ctctatgtgt tctccgcgcc ggatgccgag cgcgagtgga ctctgggcgc
     3661 cggcgtgctc ttcgggctga ccgagtcgtc gcccgacacg gcgctgcgca tcaccttcgc
     3721 ggtggagtat tgacgatgct ccgcaccacg caccgccgcc ttcgcctcct ccgcgtcgcc
     3781 tcagccgcca cgattgcgat cgccgccgcc actcctgcgt ttgcggagtt cgagattcaa
     3841 gaatcgacca tcgatcccgg tgagatccaa ctgcaatatc gcggggcgtg gcactcaggt
     3901 gtgccggctg cagatttgga cgacgacgtt gaccttggtt taatcatcga tgaacccgag
     3961 gcgctcttgt tgcagagtca cgagtttgag tttcaaatga gcgtgacgag ccgctggctt
     4021 ctcgcactta cgcacgcgtt tgccgagccc gtaggggatc acttccaact cgcggccatc
     4081 gagtttgaga gtcagatcga gttcatcgaa cgcgagggcg atggtgttgg gcttgccatg
     4141 cagggcggga tggaaaagcc agtgcaagag gcgtcagaga aattcgatcc cgattttcat
     4201 tttggaccga ttgtcgagtt gtcgaagtca aacttcacgc tcgtcacgaa cccgctcttc
     4261 ttcaagctga ttggagatct caatcggcaa gaaggttggg gctttgagta cggttggcaa
     4321 ggacgttacg aagcgtttgg cggccgtcta tggcttgcag tcgaaatgtt tggcgaaatc
     4381 gaggacatgt ctaatgcagg gccgtttgac cagcagcttc atagcctcgg tccaacattc
     4441 tattacacct ttggcaagga tgacgatgag gagggtgccg gagacgaaga cgaggacatc
     4501 tatcagaaga gccgggaact caccgtgagt ctcggcgccc agtttggctt gaccaaagaa
     4561 gcttcagacg tcgcgctcaa agtgttcatc ggctacgagt tcaactgact cgcagggcct
     4621 ctcgcgctag cggcgcacgg agaatttaaa cggcacggag acctcgacgg tgtccttgcc
     4681 aaccgccgag gggatcggcg ggaagggcgc ggccttcttg atcgagtcga ttgccgcgtt
     4741 gtcgagaacc ttggacccgg aactcttcac aatcctttgc gccacaagca cgccatcgga
     4801 tttgaccgtg aggcgaacta cggcggtgcc gatcaaagta gtgcgcgggt tcaccttggc
     4861 tttttcaaga tgcgtccgca gctgtccgag atactggcga tgggcggtcg cgtcgccgcc
     4921 cttcttctct tcgcccgtgc tctcgcgcat agcgatgacg gtctcttgcg gaatggtcgg
     4981 catttgctgc gggagcgttt gctctttgac ttcgggctcg ggctccttca actcttcctg
     5041 caccggctcg atcaactcgc tctcgggacc ggcctcggag gcgatgacct cgtcctcgac
     5101 gggctcgatc tcggacacct cctcgacagg aagctcctct tgctcctcta ccgcttcgac
     5161 ctgctcgagg ggctgtgccg cggccatcat catgggcggg gcctcaacgg cgtccacgga
     5221 gatcacgtcc tcacccagct tggcaaagcc ctgcaaggcg acgccttgct cgacaaccat
     5281 gatgtcgtcg ccggtgccct cttcgagagc gacacccccg gacgcgggca tcagcatggc
     5341 aagcgcaaag gcgccgtgaa cgccgagaga aataagccag gttatgatgc ggatcgccat
     5401 gaagttcgcc ttacaacgtg cctactggct ttgccccgcc gctcccccgt tggctttcgg
     5461 ttgggacagc agggaaacgc gcgcatagcc gctttggcca acgcgaccga ggatatccat
     5521 cacctcgcca tagggacggc tcttgtcgcc ccggacgtat acgaccgtgt cgccttcggc
     5581 ttggcgcagt gcgatcagcg tcggcacaag tgtgtcttcg gtcacttgct cttcgcggac
     5641 atagattgaa ccgtcggacg caagcgagac gatcatcggt tttttcggct gactgacttt
     5701 ggcagcggat gtgctcggca gatcgacagg gacgccggcg acgagcagcg gcgccgtcac
     5761 catgaagatg atcagcagca ccagcatcac gtcgaccatc ggcgtgatgt tgatctcgga
     5821 gatcggacgg tactcggcgt cttccccgcc accgttatta ccgagggaca tacccattac
     5881 gaacgtttcc ctgcgcgaag aggctcgacg ttggttccgc gccggccctg gggcttttgc
     5941 tccgtctcgc cggccacgag gtcgcgggcg atgtcgatca ccgcgctatt gccacgggcg
     6001 aaggaacggc caagcgccac ggtgatttgg ttgtaggcga ccaccgccgg aatggccgcg
     6061 gcgagaccaa gcgcagtcgc gatcaaagcc tcggcgatac caggtgccac gaccgcgagg
     6121 ctcgtgtcct tctgcgtcgc gatggctgtg aagctgttga cgatacccca aaccgttccg
     6181 aacaagccga taaagggtgc ggcggaaccg atcgtggcga ggaacggaag cccgacttcc
     6241 aatttctgga tctgagactt caacgagaag cgcatggcat tctcggcgcg gtagcgccgc
     6301 tcggtgggcg tgtcgtcgag atctgcatcg agctcgcgtt cgccagcctc gagcaccgtg
     6361 cggctcaggc cgccgcgtgc ccggctgtcc cggctttggg ccaggcgccg gatcgccccg
     6421 tttagccagc caatgcgaac cagtttctcg aagataatgg cccaacaggc gatcgacatc
     6481 aaaacgagca ggatcatcac gcccttgacg accggatccg cctgcatgac caagccccag
     6541 actgagtagt cgtgggtggt cgcggcagtg actgtaattt cttcttccac ggggcgcgtc
     6601 cttacttggt caacggatta ttggttcggc ttgaaaccga aatatcctgc aaacagtcgg
     6661 tggcgtcacc ggtgctggcc ttgcattcca taacgtcgtt aatgagaaag ctgccaacgt
     6721 cgtcacaggc ggtgtcggc
//
LOCUS       DBNHVU010000159         4467 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1915070, whole genome shotgun sequence.
ACCESSION   DBNHVU010000159 DBNHVU010000000
VERSION     DBNHVU010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1915070"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /locus_tag="AC16Y7_06440"
     CDS             complement(<1..1269)
                     /locus_tag="AC16Y7_06440"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR5709054.1"
                     /translation="MTTTTAAQQFVNVGERTNVTGSAKFRKLIEANDYQAALDVARQQ
                     VEAGASIIDVNMDEGLLDSEAAMETFLNLIASEPDISRVPIMIDSSKWTVIESGLRCV
                     QGKSIVNSISLKEGEDAFLEQARKVRRYGAAVVVMAFDEQGQADTKERKVAICERAYK
                     ILTEQVGFPPEDIIFDPNIFAVATGIEEHNGYGIAFIEATREIKKRMPLVHVSGGVSN
                     LSFSFRGNEPVRQAMHSVFLYHAIQAGMDMGIVNAGQLTLYDDIPDELRTLCEDVILN
                     RSPDATEALLEAAPRFKGDGGPRKSATDLSWREKPVNDRLTHALVHGITEFIEADTEE
                     ARQNADKPLHVIEGPLMDGMNVVGDLFGAGKMFLPQVVKSARVMKQAVGYLQPFLEAE
                     KKAQGLENKPAAGKILLATVKGDVHDIGKNI"
     gene            complement(1353..2483)
                     /locus_tag="AC16Y7_06445"
     CDS             complement(1353..2483)
                     /locus_tag="AC16Y7_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR5709055.1"
                     /translation="MRTQQQTRTLEMPQPAERSKKIERSMPQPDVTKRLEAAASERIL
                     ILDGAMGTMIQRHKLEEDGYRGTRFKDWQQDVKGNNDLLVLTQPAIIRDIHTSYLEAG
                     ADIVETNTFNAQRISMADYGMEDLSYEMNVEAARLAREAADAIATPDRPRFVAGAVGP
                     TNRTASISPDVNNPGYRNVNFETLREAYKEQVLALIEGGSDIILIETIFDTLNAKAAG
                     FATLEAFEEAGMRLPLMLSGTITDRSGRTLSGQTAEAFWYSVRHLEPFSIGLNCALGA
                     ELMRPYLAELSHIADARISAYPNAGLPNAMGEYDQQAEEMAAAIKPWAEDGLLNIIGG
                     CCGSTPDHIRAIAETVADYPPRQPPAIPPKLRLSGLEPFVHG"
     gene            complement(2480..3406)
                     /gene="metF"
                     /locus_tag="AC16Y7_06450"
     CDS             complement(2480..3406)
                     /gene="metF"
                     /locus_tag="AC16Y7_06450"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HIR5709056.1"
                     /translation="MTATSERKSRLLGAGEIDVSFEFFPPKSEKMERDLWTSIKRLAP
                     LCPEFVSVTYGAGGSTRERTHATVSKIMKETGLRPAAHLTCVNASKDEVNAVARDYWN
                     AGVRHIVALRGDPPQGPGTGYQPHPGGYKNAADLVAGLKDIAGFEISVAGYPERHPES
                     PTIAADIENLKAKVDAGADRIITQFGFDNTHYLRFVERARAAGIWVPITPGIVPIHNF
                     RQVAGFAIRAGASVPAWLARRFEGLDEDKTTTYLVAAAVATEQVMDLVDEGVKAFHFY
                     TLNRADLVYAICHLLGLRPLKEKPLEARAAAN"
     gene            complement(3403..4425)
                     /locus_tag="AC16Y7_06455"
     CDS             complement(3403..4425)
                     /locus_tag="AC16Y7_06455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5709057.1"
                     /translation="MPERFATDDLVNALKAAGEPTRLRILTLLAADELNVKDLTQILG
                     QSQPRISRHLKLLVEAGLVERFREGSWVYFHVSERSHGGHLARQILNMIETSDLTLRR
                     DQQRLSALKAERQDKAQAYFAANAHEWDKIRALHIPEGDVENAMSEALGPGPFNFFLD
                     LGTGTGRMLEVFANRYERGLGLDVNQTMLSFAQSKLTTAGLSHAELRHGDLYDLPLAN
                     AAADAVVMHQVLHFLSEPEQAIEEAARVLAPGGKLIIADFAPHELEFLREQHAHERLG
                     FAEDQVQAWLQAAGLEVSRPAALSPAKDHDSQRLTVTIWTAVNVPETAAQLSRGPAPK
                     SKELQT"
BASE COUNT          812 a         1200 c         1451 g         1004 t
ORIGIN      
        1 gatgttcttg ccgatgtcgt gaacgtcgcc cttgacggtg gcgagcaaaa tttttccggc
       61 ggctggcttg ttttcgagtc cctgcgcctt cttctccgcc tccaggaaag gttgaagata
      121 gccgaccgcc tgcttcatga cccgcgcact cttgaccacc tgcggcaaga acatcttgcc
      181 tgccccgaac aagtcgccaa cgacgttcat gccgtccatc aatgggcctt caatgacgtg
      241 cagcggcttg tccgcatttt gtctcgcctc ttcggtatcg gcctcgatga actccgtgat
      301 gccgtgaacg agagcatgcg tcaggcgatc gttgacaggc ttttcgcgcc aggagagatc
      361 ggtggccgat tttctcgggc cgccgtcgcc tttgaagcgc ggcgccgcct cgagcaaggc
      421 ctcggtagcg tcgggagagc gattgaggat gacgtcctcg cagagcgtgc gcagttcgtc
      481 tggaatatca tcgtagagag tgagctggcc tgcgttgaca atgcccatgt ccatgccggc
      541 ctggatggcg tgatacagaa ataccgaatg catcgcctgg cggacaggtt cgttgccgcg
      601 aaatgagaaa gagagattcg agacaccgcc cgagacgtga acgagcggca tccgcttttt
      661 gatttctcgg gtcgcctcaa tgaaggcgat gccgtagcca ttgtgttcct cgatgcctgt
      721 ggcgacggcg aagatgttgg ggtcaaagat gatgtcttcg ggcggaaagc cgacctgttc
      781 ggtcaggatc ttgtaagcac gttcgcagat cgcgaccttg cgctccttgg tatctgcctg
      841 tccctgttcg tcaaatgcca ttacaacgac tgcagcgccg tagcggcgga ccttgcgggc
      901 ctgttcgagg aacgcatctt cgccttcttt cagcgaaatg gaattaacga tggatttgcc
      961 ttgcacacag cgcaggccgg attcgatgac ggtccacttt gaactgtcga tcatgatcgg
     1021 gacgcgcgaa atatcaggtt cagaggcgat gagattgagg aacgtttcca tcgctgcttc
     1081 tgaatcgagc aaaccttcgt ccatgttcac gtcaatgatc gaggcgccgg cttcgacctg
     1141 ctggcgggcg acgtcgagcg cggcctgata gtcgttcgct tcaatgagct tgcggaactt
     1201 ggccgaacca gtgacgttgg ttctttcgcc gacgttgacg aattgttggg cggcggttgt
     1261 ggtcgtcatg atgtgaagcg ttcttttcgc tttttacggt tgataggtat ggcggtttgc
     1321 gcgctttgtt ctgttgatgg gccgcgggtg gcttagccgt gaacgaaggg ttcaaggccg
     1381 gagaggcgca gctttggcgg gatagcaggc ggttggcgcg gcgggtagtc tgcgacagtc
     1441 tcggcgatag cgcggatgtg atcgggcgtt gagccgcagc agccgccgat gatgttgaga
     1501 aggccatctt cggcccaggg cttgatggca gccgccattt cttcggcctg ctggtcgtat
     1561 tcgcccatgg cgttgggaag cccggcattt gggtaggcgg aaatgcgagc gtccgcgata
     1621 tgagaaagct ctgccagata cgggcgcatc agttctgcac cgagcgcaca gttgaggccg
     1681 atcgaaaacg gctcgaggtg gcgaacggaa taccagaagg cttcggctgt ttggccggag
     1741 agcgtgcgcc ccgagcggtc ggtgatggtg ccggacagca tcaagggcag cctcatccct
     1801 gcttcctcga aggcttccag ggtggcgaag ccggcagcct tggcgttcaa ggtgtcgaaa
     1861 atcgtttcga tcaaaataat gtccgacccg ccttcgatca gcgccagcac ctgttccttg
     1921 taggcttcgc ggagcgtttc gaagttgacg ttgcggtagc ccgggttgtt gacgtcggga
     1981 gagattgagg ctgtgcgatt ggtcggaccc actgcgcctg caacgaagcg cgggcggtct
     2041 ggtgtcgcga tagcgtctgc cgcctcgcga gccagcctgg cggcctcgac gttcatttcg
     2101 tacgagaggt cctccatgcc gtaatcagcc atggagatgc gctgcgcatt gaaggtgttc
     2161 gtctcgacga tgtcggcgcc agcttcgaga tagctggtgt ggatgtcgcg gatgatggcg
     2221 ggctgggtca aaaccagaag gtcattgttg cccttcacat cttgttgcca gtccttgaaa
     2281 cgggtgccgc gatacccgtc ttcttccagc ttgtggcgct ggatcatggt gcccatggcg
     2341 ccatccaaaa tcaggatgcg ctcgctggct gcggcttcca atcgtttcgt cacatcaggt
     2401 tgcggcatcg atctctctat ctttttcgac cgctcagcag gttgcggcat ttcgagtgtt
     2461 cgggtttgct gttgggttct caatttgcgg cagcgcgggc ttccaatggt ttttctttca
     2521 aagggcgcag gccaagcaag tggcagatgg catagacaag atctgcgcga ttgagggtgt
     2581 agaagtggaa cgccttcacg ccttcatcca ccaggtccat cacctgctct gtggcaacgg
     2641 cggcggcgac gagataggtg gtggtcttat cctcgtccaa accttcaaat cgccgcgcca
     2701 gccaggccgg tacactcgcg cctgctctga tggcaaagcc tgcgacctgg cgaaagttgt
     2761 ggatgggaac gatgccaggt gtgatcggca cccagatgcc ggcggcacgg gcgcgttcga
     2821 cgaagcgaag atagtgggtg ttgtcgaagc cgaattgggt gatgatgcga tcggcaccgg
     2881 cgtccacctt ggccttcagg ttttcaatgt cggcagcaat ggtggggctt tccgggtggc
     2941 gctcgggata accggcgacg gaaatttcaa agcctgcgat gtctttcagg ccggcaacca
     3001 ggtcggcggc gttcttgtaa ccgccagggt gtggctgata tccggtgccc gggccttgcg
     3061 gcgggtcacc gcgcagagca acgatgtggc gaacgccggc gttccaatag tcccgggcaa
     3121 cggcgttgac ctcgtccttg ctggcgttga cacatgtgag gtgggcagca ggcctcagac
     3181 cggtttcttt catgattttg gaaacggtgg cgtgggtgcg ctcgcgcgtc gaaccgccgg
     3241 cgccataagt gaccgaaacg aattcggggc acaacggggc gaggcgcttg atcgaggtcc
     3301 agaggtctct ttccatcttc tcgctcttcg gtggaaagaa ctcgaaggag acgtcaatct
     3361 cgccggcgcc gaggagacgg ctcttgcgtt cgcttgtggc ggtcatgttt gcagttcctt
     3421 tgatttcggt gccgggccgc ggctgagttg agcggcggtt tccggcacat tgactgcggt
     3481 ccaaatggtc acggtgagac gttgactgtc atgatccttg gcaggtgaaa gtgcggctgg
     3541 cctggagacc tcgaggccgg cagcctgcag ccaggcttgg acctggtctt cggcaaagcc
     3601 gaggcgctca tgggcgtgct gttcgcgcag gaactcaagc tcatggggtg cgaaatcagc
     3661 aatgatgagc ttgccgcccg gcgccagcac gcgggcagct tcctctatgg cctgctcggg
     3721 ttcagacagg aagtgcagca cctggtgcat gacaacggca tcggcggcgg cgttggccaa
     3781 gggtagatcg taaaggtcgc catggcgcag ttcggcatgg ctcaatccgg ctgtggtgag
     3841 tttggattgg gcaaaggaca acatggtttg attgacgtcg agccccagac cgcgctcgta
     3901 gcggttggca aagacttcga gcatgcggcc ggtgcctgtg ccaaggtcta ggaaaaagtt
     3961 aaagggaccc gggcccagag cctcggacat ggcgttttcg acgtcgcctt cgggaatatg
     4021 gagcgcgcga attttgtccc actcgtgggc attggcagca aaatatgcct gagccttgtc
     4081 ctggcgttcg gccttcaacg ccgacaagcg ttgctgatcg cggcgcagcg tcaggtcgct
     4141 tgtttcgatc atgtttagaa tctggcgcgc cagatggccg ccgtggctgc gctcggagac
     4201 atggaagtaa acccaactgc cctcgcgaaa gcgttcgacg aggcctgctt caacgagcag
     4261 tttaagatgg cgggaaatgc gtggttggct ctgacccaaa atctgggtca agtcttttac
     4321 attgagctcg tccgccgcca acagcgtcag gatgcgcaga cgtgtcggtt cgcccgcggc
     4381 ctttagggcg ttgacgaggt catcggtggc aaatcgttcc ggcatggcgc tgtggctagc
     4441 ttcccgggct ggtcgtaaac tgctgca
//
LOCUS       DBNHVU010000160        12141 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1767756, whole genome shotgun sequence.
ACCESSION   DBNHVU010000160 DBNHVU010000000
VERSION     DBNHVU010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 12141)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12141)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12141
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1767756"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /gene="glmM"
                     /locus_tag="AC16Y7_06460"
     CDS             <1..1226
                     /gene="glmM"
                     /locus_tag="AC16Y7_06460"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR5709058.1"
                     /translation="SGYMIESAMVSGFTAVGMDVFQLGPMPTPAVAMLTRSLRADLGV
                     MISASHNPYIDNGIKLFGPDGYKLSDEQEAEIEALMEGDAAALLAPPEHIGRATRIDS
                     ARERYVEFAKRTLPRNLRFDGIRAVIDCANGAGYKVAPEALWELGTEVIKIGVEPNGL
                     NINKDCGSTAVQNLIRKVSEVRADIGIALDGDADRVVIVDEKGRIIDGDQLMAVIAEF
                     WLRRGRLAGGGVVATVMSNLGLERFMEDLGLGLARTSVGDRYVTEHMRQHGYNVGGEQ
                     SGHIVLSDFSTTGDGLVTALQILAVVAESGKPVSEVCNRFEPLPQVLESVRYKNGQPL
                     DNLGVQRAINDGKSRLGTAGRLVIRPSGTEPVIRVMAEGDDEGLVKTVVSDIVHALSD
                     AAAECRRSRASAAAY"
     gene            1243..1980
                     /locus_tag="AC16Y7_06465"
     CDS             1243..1980
                     /locus_tag="AC16Y7_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HIR5709059.1"
                     /translation="MGCTGPVYLKGFVGAANPVVGNISTEPFQYNDFIVEHEGIKSSP
                     LFGVGIGYQFNTWLRFDMTGEYRGDALFVAHDRYPGGNGTFNRGSNDFAGEFLPGTNE
                     YTANMKTWLGLFNAYVDLPTVLCVTPYVGAGIGMSSISVLGLKDVNVPNNSVFYGADN
                     TETNFAWAVYGGLAYDVNPSVTLDLSYRYADLGDARSGTVTAYDGSSSYAGVNIDDVT
                     SHDLMLGVRWKLGHTVAAPVPMPVAFK"
     gene            2142..3317
                     /locus_tag="AC16Y7_06470"
     CDS             2142..3317
                     /locus_tag="AC16Y7_06470"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HIR5709060.1"
                     /translation="MTLPPKPTKRPENPRFSSGPCTKHKGWSLDALKGALLGRNHRGP
                     ETKARIEAALLKTRALLQIPDAYRVALVPASDTGAVEMALWSLLGPRGVDVLAWEAFS
                     SDWVTDIVEQLKLTDVRALVAPYGALPNLADVDFDRDVVFAWNGTTSGVRVPNAEWIA
                     ADRAGLTICDATSAVFAQDIDWTKLDVATYSWQKVLGGEAQHGMLILSPRAIERLESH
                     TPSWPLPKLFRLTKDGKVMQDLFEGATINTPSMLCLEDYHDALSFVERIGGLEGAIGR
                     ADANAKVIADWVAKTPWVDFVAVEPATRSNTSVCLKIVDPEIAALSADLRDGFPRAMA
                     ARLEEEGVAYDIASYRSAPAGLRIWTGATVETDDVAALMRWLDWAFAVEKAASSKAA"
     gene            3430..4728
                     /locus_tag="AC16Y7_06475"
     CDS             3430..4728
                     /locus_tag="AC16Y7_06475"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR5709061.1"
                     /translation="MANVVVVGSQWGDEGKGKIVDWLSERADVVVRFQGGHNAGHTLV
                     VDGTTYKLSLLPSGVVRKNKLAVIGNGVVVDPWALADEIDRIQVLGVTIGCDNLRIAE
                     NATLILPLHRELDALREAAAGAGKIGTTKRGIGPAYEDKVGRRAVRVMDLADLPSLKP
                     KIERILAHHNPLRRGLGEPEISADELCSQLTQIAPKILPYVDRVWTLLDHARRDGKRI
                     LFEGAQGALLDIDHGTYPFVTSSNTVAAQAATGAGVGPGAINYVLGITKAYTTRVGEG
                     PFPTELMDETGKLIGERGAEFGTVTGRARRCGWFDACLVRQTCKVAGINGIALTKLDV
                     LDDIPGGTLKICVGYKLDGEEIDYLPAAQGAQGRVEPIYEEMEGWTESTHGARSWAEL
                     PAQCIKYVRRLEELTGVPVALLSTSPRREDTILVHDPLVD"
     gene            4737..5510
                     /locus_tag="AC16Y7_06480"
     CDS             4737..5510
                     /locus_tag="AC16Y7_06480"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5709062.1"
                     /translation="MADTGYPWTVVWITGASGAICGEIARRLAAKGVKVAATARPSER
                     LDALAASSPNIAAFPADVLKPDELKTCAAKVEAELGQIDLALFGAGMYVPFDIRNIDL
                     DGFRKTMALNVDGVINGVAAVLPNMLERRAGHLAIMGSLFGYAGWPGNGSYGASKAAV
                     INIAESLKLELRGTGVDLTLISPGFVDTPLNAAYDPKKKLFVMKKERCARKILAKLPK
                     RPYEIAFPIQVEGFLKTVRDMPRFASFPVVRWFIKATGA"
     gene            complement(5515..6501)
                     /locus_tag="AC16Y7_06485"
     CDS             complement(5515..6501)
                     /locus_tag="AC16Y7_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5709063.1"
                     /translation="MEHIKIAGLPFDASRIALGTWAIGGWMWGGTDERDAIDTIHRAL
                     DLGINLIDTAPVYGFGQSEEIVGKALEGRRDKVLIATKAGLDWSGEGIVRNSARARLR
                     QELEDSLRRLKTDIVDVYQIHWPDEGTDFAETARTLEGLRREGKIRAIGVSNFSPEQM
                     ERFKSEVPLATVQPPYNLFEREIEDYVLPYAKAHHIVVLAYGALCRGLLTGKITADTT
                     FKGDDLRNIDPKFKPDRRGQYLAAVDKLSALAKARFGKPVVALAARWILDQGDTIALW
                     GARKPLQLDAIGDVMGWHIDARAMTEIDKIFAETVTNPVGPEFMAPPRTNVA"
     gene            complement(6504..7868)
                     /locus_tag="AC16Y7_06490"
     CDS             complement(6504..7868)
                     /locus_tag="AC16Y7_06490"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR5709064.1"
                     /translation="MDTQQDVTVKSWDGTWTYHPRVRVTPESVDDLVEIMTDTVRFPT
                     PVRPAGSMHSTARMNGDDEGGTMVDMKAMNRILHFSEDTVTVEPGVTHDELTRALKAR
                     GLQPYITTEIGNTTIAAFACAATKDSSFPGGFGQVSSYVTAVRLVTPQGEIREISEAD
                     NPKEMQFLRSSYGLFGLIFEVTIKVRPTTAMSMRHYSMTLESFRKFFPVYKARGYAVM
                     YYIFPYAKRVVVELRKDNPEVEPKSRLQWAYRNRFWRKYAPAVTLWIDKNVTNRKWHA
                     RAQDFFFYTLRQLMVWFVRSERSWPHAQIINYPRNPGKTKYLFSMWSFREENFFDILE
                     DYCDFCIFHESQTGFRCNLPSVGYAICRDCEALLSYTWEGPGMSIDPASTGGPEWEEF
                     LLSYNAFCSERGGTPLFNQTPFLNREMARRAFGPRLQQFAARRQELDPDGRLLDQHFR
                     ELLS"
     gene            8111..9481
                     /locus_tag="AC16Y7_06495"
     CDS             8111..9481
                     /locus_tag="AC16Y7_06495"
                     /inference="COORDINATES: protein motif:HMM:NF012946.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR5709065.1"
                     /translation="MKRYCIIGAGACGLPVAKTFAERGIPFDCFEAEGDVGGIWNPRS
                     PHAVYGSTYLNSSKKLSRYPDFKLPDEMPHYLSASQAQAYLRAYATEFGLYEHITFNT
                     RVASVERADDGWRVTLQGEDKPRIYGGVVVANGHHWHAFTPDYPGTFDGEMLHSHDLK
                     SKAQLKDKRVLVVGAGNSAVDILDDAAHDGNAAIHSMRRSYYFFPKLLFGKPTDSVID
                     KLSHVPLPRPVMYWLYKLGMKILIGSHHRFGLPDPDHSLFEAHPTACTTYLDHIVHGR
                     VTTKPGIKRLEGKRVYFEDDSSEEIDLLVWATGFRVNFPFMDDSYVLDEKGWPKLFIH
                     TFHREWDDFFACGLFEPAEGGVWQIADFQARLIAAFIVAQDEDKARADWFRKLKSHAK
                     PDIGHGIKTKDTPWHRFEIHHLRFRRYMRRLLKKFGACAGPKPEMRTMGESAEEREDE
                     KLKLAS"
     gene            complement(9591..9938)
                     /locus_tag="AC16Y7_06500"
     CDS             complement(9591..9938)
                     /locus_tag="AC16Y7_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HIR5709066.1"
                     /translation="MFEPFDKATKDFQKLGKDNTDAIARSYAELNKGFQTIGTGLTDY
                     SKQAFEDATATFEKLVGSKSLEQAVEIQSQYAKKAYDTWVAEASKIGEMYASVARNAY
                     KPVAKAPQVTTTE"
     gene            complement(10056..10415)
                     /locus_tag="AC16Y7_06505"
     CDS             complement(10056..10415)
                     /locus_tag="AC16Y7_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR5709067.1"
                     /translation="MNRDPRSPCPIATTLDIVGDRWTLVVVRDLLTGKKRFSEFLDSP
                     EGIATNVLSDRLALIEQAGLVTKAPYQLRPKRFEYTLTTKGEDLLPTLQEMSRWANRH
                     IRGTWTPPESFMAKKIP"
     gene            10508..10954
                     /locus_tag="AC16Y7_06510"
     CDS             10508..10954
                     /locus_tag="AC16Y7_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014283768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HIR5709068.1"
                     /translation="MAEGFSGKCLCGAVHYESSADPQFIGQCHCIDCRKTSGTDHATH
                     IAIPEDAFTVTGTLKFFDHPANSGNIVGRGFCPECGAPIYSRNSAMKGLVFVRASSLD
                     DLEIAKPQVIVFASRAPSWRHLDPELPAYAEMPEKMPKAVSKPASS"
     gene            11121..>12141
                     /locus_tag="AC16Y7_06515"
     CDS             11121..>12141
                     /locus_tag="AC16Y7_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012861.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIR5709069.1"
                     /translation="MAPLIGLATGVAAILFRLAIGVFQWPWLGTMSENVAMAARAQPW
                     WVIMLAPAIGGLLVGLLLRYVLTARRTDAVPDVMEARVNAGRGMKFGQGLLSALTSAL
                     SLGAGASAGREGPIVHLGAALAACGSTHFNLPNASRRTLLASGAAGAVAASFNAPIAG
                     VLFAQEVILGHFSITTFVPLVLSSVVATIISQAWFGDVAAFIVPSYRIVSYLEVPAFV
                     LLGIVAAAVAVIFQLTILSVDWLARQVKMPLVSRPMVGGLLIGIMAIWFPEVLGVGYD
                     TTDAALKGQLAIHVMIILIAVKTIATAIALSSRFGGGVFSPALYLGAMTGGAFGLIAG
                     GIFPEV"
BASE COUNT         2130 a         3697 c         3923 g         2391 t
ORIGIN      
        1 tgtccggcta catgatcgaa tccgccatgg tgtcgggctt caccgccgtg ggcatggatg
       61 tattccagct cgggccgatg ccaacgccgg ccgtggcgat gctgacccgc tccttgcgcg
      121 ccgatcttgg cgtgatgatt tcagcttctc acaatccgta tatcgacaac ggcatcaagc
      181 tgttcggacc ggacggctac aagctttccg acgagcagga agccgagatc gaagcgttga
      241 tggagggcga tgctgcagcg ctgctggcgc cgcctgagca tatcggccgc gcgacccgca
      301 tcgacagtgc gcgcgagcgc tatgtcgagt tcgccaagcg cacgctgccc cggaatttgc
      361 gcttcgacgg catccgcgcc gtgatcgatt gcgccaacgg ggccggctac aaggtggcgc
      421 ccgaggcgct gtgggaactc ggcaccgagg tcatcaagat cggggtcgaa ccgaacggcc
      481 tcaacatcaa caaggattgc ggctcgacgg cggtgcagaa cctgatccgc aaggtcagcg
      541 aagtacgcgc cgatatcggt atcgcgttgg acggcgacgc cgaccgcgtg gtcatcgtcg
      601 acgagaaggg ccgcattatc gatggggacc agctgatggc cgtgatcgcg gagttctggc
      661 tcaggcgtgg acggctcgcc ggcggcggcg ttgtcgccac ggtcatgtcc aatctcggcc
      721 tggagcggtt catggaggat ttgggcctcg ggctcgcgcg cacgtctgtg ggcgaccggt
      781 acgtgaccga gcatatgcgc cagcacggct acaatgtcgg aggggagcaa tccggccata
      841 tcgtgctgtc ggacttctcg accaccggag atggcctggt gacggcgctg cagatcctcg
      901 ccgtggtagc cgagagcggc aagcccgtga gcgaggtctg caaccggttc gagcccttgc
      961 cgcaagtgtt ggaaagcgtg cgctacaaga acggccagcc gctcgataat ctgggtgtgc
     1021 agcgggcgat caacgacggc aaatcgcggc tgggcaccgc cggacggctg gtgatccgcc
     1081 cgtccgggac cgagccggtg attcgcgtga tggcggaagg cgacgacgag ggcctcgtga
     1141 agaccgtcgt ctccgatatc gtgcacgcgc tgtccgacgc cgccgcggaa tgccgccgat
     1201 ctcgggcttc cgccgccgcc tattgaggtg gctgcgcctt gcgtggggtg cacggggccg
     1261 gtctacttaa agggcttcgt cggggccgcc aacccggtgg tcggcaacat ttccaccgag
     1321 cccttccaat acaacgattt catcgtcgaa catgaaggca tcaagagctc gccgctcttc
     1381 ggtgtcggta tcggctacca gttcaatacc tggctgcgct tcgacatgac cggtgaatat
     1441 cgcggcgacg ccttgttcgt tgcgcatgat cgttacccgg gcggcaacgg cacgttcaac
     1501 cggggcagca atgacttcgc aggtgagttc ctgccgggta ccaacgagta cacggcaaac
     1561 atgaaaacct ggctcggcct gttcaacgcc tatgtcgatc ttccgaccgt cttgtgcgtc
     1621 acgccttatg tcggcgccgg tatcggtatg tcttcgatct cggttctcgg tctcaaggac
     1681 gtgaacgtgc cgaacaattc ggtgttctac ggagccgata acacggagac caacttcgcc
     1741 tgggccgtct atggcggtct cgcctatgac gtgaacccgt ccgttacact ggacctctcc
     1801 taccgctatg ccgatctcgg cgatgcacgg agcggcacgg tcacggccta tgacgggtcg
     1861 agctcgtatg cgggcgtgaa catcgacgac gtcacctcgc atgacttgat gctcggcgtg
     1921 cgctggaagc ttggtcacac agtggccgcg cctgtgccga tgcccgtcgc gttcaaataa
     1981 tttaccaccc acccaccaca ccaactgccg cccgttcttt cgatgggcgg cttttctttg
     2041 tgatggcggc gcatccggcg cgcccttgac ggcgatttcc gccccccgta tgggcattcg
     2101 agccccctta tgcgggggct cgttctttcg gagtttcgcc tttgaccttg ccgccaaaac
     2161 cgaccaaacg gccggaaaat ccgcgctttt cctcgggccc gtgcaccaag cacaaaggct
     2221 ggtcgctgga cgcgctgaag ggcgcgcttc tggggcgcaa tcatcgcggg cccgaaacca
     2281 aggcgcggat cgaggccgcg cttctcaaga cgcgcgcgct gcttcaaatt ccggacgcgt
     2341 atcgggtggc gttggtgcct gcctccgaca cgggcgccgt ggagatggcg ctgtggtcgc
     2401 tgcttgggcc gcgcggtgtc gacgtgctgg cttgggaagc cttcagcagc gattgggtca
     2461 cggacatcgt cgagcaattg aagttgacgg acgttcgcgc gcttgtcgcg ccctatggcg
     2521 cgctgccgaa ccttgccgac gtcgacttcg accgcgacgt ggtctttgcc tggaacggca
     2581 cgacgtcggg tgtgcgcgtg ccgaacgccg agtggatcgc ggcagaccgc gcgggcctca
     2641 ccatttgcga cgcgacatcc gccgtgttcg cgcaagacat agattggacg aagctcgatg
     2701 ttgcgaccta ttcttggcag aaagtgctgg gcggggaggc gcagcacggc atgctgatct
     2761 tgtcgccgcg cgcaatcgag cggttggaaa gccacacgcc gtcatggccg ctgccgaagc
     2821 tgttccgcct aacgaaagac ggcaaagtga tgcaggacct gttcgagggc gcgaccataa
     2881 acacgccgtc catgctttgc ctggaggact atcacgacgc gctgtctttc gtggagcgta
     2941 tcggcggact ggagggcgcg atcggccgcg ccgacgccaa tgcgaaagta attgcggact
     3001 gggtggcgaa gacgccgtgg gtcgacttcg tggcggtaga gcctgcgacg cgctccaaca
     3061 cgtccgtgtg tcttaagatc gtggaccccg agatcgccgc tctttcggcc gatttgcgcg
     3121 atggctttcc gcgggccatg gcggcgcggt tggaggagga gggcgtcgcc tacgacatcg
     3181 cctcctatcg cagcgccccg gcgggcttgc gcatctggac cggcgccaca gtcgagaccg
     3241 acgatgtggc ggcgctgatg cgctggctcg actgggcctt tgccgtggag aaggcggcaa
     3301 gctccaaagc ggcctagagc gtcgccggaa ggctcgcatc ccgcgcgcgc ccacgctata
     3361 acgggacacg gcccggcacg tttgcggggc cgtttctgtg tttcttccag gatttagggg
     3421 attttcgaag tggccaatgt tgtggtcgtc ggctcccaat ggggcgatga gggcaagggc
     3481 aagatcgtcg actggctgtc agagcgcgcc gacgtggtgg tgcgtttcca gggcggccac
     3541 aatgccggcc acacgctggt ggtcgatggg acgacctata agctctcgct cctgccctct
     3601 ggtgtggtgc gtaagaacaa gctcgccgtc atcggcaacg gggtcgtggt cgatccttgg
     3661 gccttggcgg acgagatcga ccgcatccag gtgctcggcg tcacgatcgg ctgcgacaat
     3721 cttcgcatcg ccgagaacgc caccctgatc ctgccgctcc accgggagct cgacgcgctg
     3781 cgcgaagctg cggccggtgc cggtaagatc ggcaccacca agcgcggcat tggtccggcc
     3841 tatgaggaca aggtcgggcg ccgggcggtg cgcgtcatgg acctcgccga ccttccgtcg
     3901 ttgaagccga agatcgagcg catcctggcg caccacaatc ccttgcgccg gggcctcggc
     3961 gagccggaaa tctcggcgga tgagctctgc agtcagctca cccagatcgc gccgaaaatt
     4021 ctgccctatg tggacagggt ctggaccctg ctcgatcatg cgcggcggga cggcaagcgc
     4081 atcctctttg agggcgcgca aggcgcgcta ctcgacatcg accatggcac ttatccgttt
     4141 gtcacctcgt cgaacacggt ggcggcgcaa gcggccacgg gcgctggcgt cgggccgggt
     4201 gccatcaact acgtgctcgg catcaccaag gcctacacca cacgggtcgg cgaggggccg
     4261 ttcccaacgg aacttatgga cgagacgggc aagttgattg gcgaacgggg cgcggagttc
     4321 ggcacggtga cgggccgggc gcgccgctgc ggctggttcg acgcctgcct ggtgcgccag
     4381 acctgcaagg tggcggggat caacggtatc gcgctcacca agctcgacgt gctcgacgac
     4441 attccgggcg gcacgctcaa gatctgcgtc ggctacaagc tcgacggcga ggagatcgac
     4501 taccttccgg ccgcgcaagg ggcgcaaggc cgggtcgagc cgatctacga ggagatggaa
     4561 ggctggacgg agtcgacgca tggcgcgcgg tcctgggccg aactccccgc gcaatgcatc
     4621 aaatatgtcc ggcgcctgga ggaactgaca ggcgttcccg tggctctgct gtccaccagc
     4681 cccaggcgcg aggacaccat cctcgtgcac gatcccttgg tggattaggc tgtcccatgg
     4741 ccgatacggg atatccttgg acggtggtgt ggatcaccgg cgcgtccggc gcgatctgcg
     4801 gtgagatcgc gcggcggctt gccgccaaag gcgtaaaggt cgccgccacg gcgcggccga
     4861 gcgagcggct tgatgcgctg gcggcttcga gccccaacat cgcggcgttt cccgccgacg
     4921 tgctgaagcc tgacgaactc aagacttgcg cggcgaaggt cgaggcggag ctcgggcaga
     4981 tcgatctcgc gctattcggc gcagggatgt atgtgccttt cgacatccga aacatcgatc
     5041 tcgacggttt ccgcaagacc atggcgctca acgtcgacgg cgtgatcaac ggcgtcgccg
     5101 ccgtcttgcc gaatatgctg gagcggcgcg ccggccatct ggccattatg ggttcgctgt
     5161 tcggctatgc gggctggccc ggcaatggca gctatggcgc cagcaaggcg gcggtgatca
     5221 atatcgccga gagcctgaaa ctggaactcc ggggcaccgg cgtcgacctg acgcttatca
     5281 gtccgggctt cgtcgataca ccgctgaatg ccgcctacga tccgaagaag aagctcttcg
     5341 tgatgaagaa ggagcgctgc gcccgcaaga ttctcgcaaa gctgccgaag cggccctacg
     5401 aaatcgcctt cccgattcag gtcgaaggct tcttgaagac cgtacgcgat atgccgcgct
     5461 ttgcgtcctt ccccgtcgtc cgctggttca tcaaggcgac gggcgcgtag cggttcacgc
     5521 cacgttcgtg cggggcgggg ccatgaactc aggccccacc gggttcgtca ccgtctcggc
     5581 aaaaatcttg tcgatctccg tcatggcgcg agcatcgata tgccagccca tcacgtcccc
     5641 gatcgcatcg agttgcaagg gtttccgcgc gccccataag gcgatggtat cgccctgatc
     5701 gaggatccag cgcgcggcga gcgccacgac cggtttgccg aatcgtgcct tggccagcgc
     5761 agagagtttg tcgacggcgg caagatattg tccgcggcgg tccggtttga acttcggatc
     5821 gatattgcgc agatcgtcgc ccttgaacgt cgtgtcggcg gtgatctttc ccgtcagaag
     5881 tccgcggcac agcgcgccat aggccagcac cacgatgtgg tgtgccttgg cgtaggggag
     5941 aacatagtcc tcgatctcgc gctcgaacag attgtagggc ggctgcaccg tggcaagggg
     6001 cacctcgctc ttaaagcgct ccatctgttc gggcgagaag ttgctgacgc cgatggcgcg
     6061 gatcttcccc tcgcggcgta atccttcgag cgtccttgcg gtttccgcga aatccgtccc
     6121 ctcgtcgggc cagtgaattt gatagacgtc gacgatgtct gtcttgagcc gccgcagcga
     6181 gtcttccagc tcttgacgca ggcgcgcccg cgccgagttg cgcacgatcc cctcgccgct
     6241 ccaatcgagc cccgccttgg tggcgatcag cactttgtcc cggcgcccct ctagcgcctt
     6301 gcccacgatc tcttcggact ggccgaaccc gtagacgggg gcggtgtcga tgagattgat
     6361 gccgagatcg agtgcgcgat ggatagtgtc gatggcgtcc cgttcgtcgg tgccgcccca
     6421 catccagccg ccgatcgccc aggtgcccag cgcgatgcgc gaggcatcga aaggcaatcc
     6481 ggcaatcttg atgtgttcca tgcttacgac agcaactcgc ggaaatgttg gtcgagaaga
     6541 cggccgtcag gatcgagttc ctgccggcgc gcggcgaact gctgaaggcg cggcccgaag
     6601 gcgcggcgcg ccatttcgcg gttcaggaac ggcgtctggt tgaacagcgg cgtgccgcca
     6661 cgctcgctgc aaaacgcgtt gtagctgagg aggaattcct cccattccgg gccaccggtc
     6721 gaggccggat cgatactcat gccggggcct tcccacgtgt aggacagcag cgcttcgcag
     6781 tcccgacaga tggcgtagcc gacgctcggc aggttgcaac ggaacccggt ctgtgactca
     6841 tggaagatgc agaaatcgca atagtcttcc aggatgtcga agaaattctc ctcgcgaaac
     6901 gaccacatgc tgaagaggta tttcgtcttg ccgggattgc gcgggtagtt gatgatctgt
     6961 gcgtgcggcc aactgcgctc ggagcgcacg aaccacacca tcagctgacg cagggtgtag
     7021 aagaagaagt cctgcgcgcg cgcgtgccat ttccgattcg tgacgttctt gtcgatccat
     7081 agcgtcaccg ccggggcgta tttgcgccag aaccggttgc gataggccca ttgcagccgc
     7141 gacttcggtt ccacttcggg attgtctttg cgcaactcga cgaccacgcg cttggcgtag
     7201 ggaaagatgt agtacatcac ggcgtagccg cgcgccttgt agacggggaa gaacttgcgg
     7261 aagctctcca aagtcatcga gtagtgccgc atcgacatcg cggttgtcgg ccggaccttg
     7321 atagtgacct cgaaaatgag tccgaacagc ccgtagctag agcggaggaa ctgcatctct
     7381 ttcgggttgt cggcttcgga gatttcgcgg atttcacctt gcggtgtgac gagacgcacc
     7441 gcagtgacgt aggagctcac ctgaccgaag ccgccgggga aagagctgtc tttggtggcg
     7501 gcgcaggcaa aggcggcgat cgtcgtattg ccgatctcgg tggtgatata gggctggagc
     7561 cctcgggcct tcagcgcgcg ggtgagctcg tcgtgagtca cgccgggctc caccgtgacc
     7621 gtgtcttcgc tgaaatgcag gatgcggttc atagccttca tgtcgaccat cgtgccgcct
     7681 tcgtcgtcgc cgttcatgcg cgcggtggaa tgcatggagc ccgccggccg cacgggggtc
     7741 gggaaccgca ccgtgtcggt catgatttcg acgaggtcgt cgacgctctc tggcgttacc
     7801 ctcacccgtg ggtgataggt ccaggtgccg tcccaactct tgacggtgac gtcctgctgg
     7861 gtgtccatga atctccctcg atgtcgcggc gacagaccat aacgaattga gcccgcgaat
     7921 ccaggctcga agcggtgtgc cgccgagacc cattgagatc gcgttcggcc ttggtgaact
     7981 tgaggcagat caatgaggta tggcggggat gctatgggtt gaaggggggc cgggtgagtg
     8041 cttaggtctg ccgtaattgg cggctatttg cggtgtagga ttttcgcgcc ggaggtttag
     8101 aatcgaggct gtgaagcgct actgcatcat aggggccggg gcctgcggcc tgcccgtggc
     8161 caaaaccttc gcggagcggg ggattccctt cgattgcttc gaggcggaag gcgatgtcgg
     8221 tggcatttgg aacccgcgca gtccccatgc ggtctacggg tcgacctacc taaattcgtc
     8281 caagaagctg tcacgctatc cagacttcaa gcttccggac gagatgccgc actatctcag
     8341 tgcgtcgcaa gcccaggcgt acttgcgtgc ttatgccaca gagttcgggc tctacgagca
     8401 catcaccttc aatacccgcg tggcttcggt tgagcgggcg gatgacggct ggcgcgtcac
     8461 gctccaagga gaagacaagc cccggatcta tggcggggtg gtggtggcca acggccacca
     8521 ttggcatgcc ttcacgcccg actaccccgg aacgttcgac ggcgagatgc tgcactcgca
     8581 cgacctgaag tcgaaggcgc aactcaagga caagcgcgta ctcgtggtcg gcgccggcaa
     8641 ttcggccgtg gacattctgg atgacgcggc gcacgacggc aacgcggcga tccactccat
     8701 gcgccggagc tattacttct tcccgaagct gctgttcggg aaacccaccg actcggtgat
     8761 cgacaagctg agccacgtgc cgctgccgcg gcccgtcatg tattggctct acaagctcgg
     8821 catgaagata ttgatcggat cacaccaccg tttcggtctg ccggacccgg atcacagcct
     8881 gttcgaagcc caccccaccg cctgcacgac ctatctcgat catatcgtgc atggtcgcgt
     8941 cacgacgaag cccggcatca agcggctcga gggcaagcgc gtgtatttcg aggacgacag
     9001 ctccgaggag atcgatctgc tggtctgggc gacggggttt cgcgtcaact tccccttcat
     9061 ggacgattcc tacgtgctcg acgagaaggg atggccgaag ctattcatcc acaccttcca
     9121 tcgggagtgg gacgacttct tcgcgtgcgg cttgttcgag ccggccgaag gcggcgtctg
     9181 gcagatcgcc gattttcagg cccggctcat cgccgctttc atcgtggcgc aagacgagga
     9241 caaggctcgc gccgactggt tccgcaagct gaagtcacac gccaagcctg atatcggcca
     9301 cggcatcaag accaaagaca cgccgtggca ccgcttcgag atccatcact tacgcttccg
     9361 ccgctatatg cgacggctgc tcaagaagtt cggcgcttgc gccggaccga aacccgagat
     9421 gcggaccatg ggcgagagcg ccgaagagcg ggaagacgaa aagctcaaac ttgcttccta
     9481 agtggcgcgt ccttgcgcgc accctttgcg tgccccctgg cttgaagaca tgaaaaaacc
     9541 cggaaggcag acttccgggt cttttgtacg ttgcgcgaag aacgcggcgc ttactcggtg
     9601 gtggtgacct gcggagcctt cgccacgggc ttataggcgt tgcgggcaac ggacgcatac
     9661 atctcgccga tcttcgaggc ttcggcgacc caggtgtcgt aagccttctt ggcgtattgc
     9721 gactggatct cgacggcctg ctcgagggac ttggagccga cgagcttctc gaaagtggcg
     9781 gtggcgtctt cgaacgcctg cttcgaatag tcggtcaggc cggtgccgat ggtctggaag
     9841 cccttgttga gctcggcata ggagcgcgcg atggcgtcgg tgttatcctt gcccagcttc
     9901 tggaagtcct tggtggcttt gtcgaacggt tcaaacatct cggaaatcct ctctccatta
     9961 gcgtttctca tgctcacggg aggatatata ttgcactgca caataaaatc aagggtaaaa
    10021 tgctgcgatg caacaaggta tgaaatctct gtaaatcagg ggattttctt ggccatgaag
    10081 ctctcgggcg gcgtccaggt gccccgaata tggcgattgg cccagcggct catctcctga
    10141 agcgtgggca gcaaatcttc accctttgtc gtcagcgtgt actcgaaacg cttgggccgg
    10201 agctgatagg gcgccttggt gacgaggccg gcttgctcga tcagcgccag ccggtcgctc
    10261 agcacgttgg tggcaatccc ttcgggagaa tcgaggaatt ccgagaagcg tttcttgcct
    10321 gtcagcaggt cgcgcacgac gaccagcgtc cagcggtcgc cgacgatatc cagcgttgtg
    10381 gcgatggggc agggagatcg ggggtcgcga ttcatttgcc ccattgtgtc acttgttact
    10441 tgcaatatgc aagtagcaat cccaggctag tatccatcgt gggggttggc agttcatgga
    10501 gggggtgatg gctgaagggt tttccggcaa gtgcttgtgc ggtgcggtcc actatgagag
    10561 ttcggcggat ccgcagttca tcgggcagtg tcattgtatc gactgccgca agacgagcgg
    10621 caccgaccac gccacccata tcgcgatccc cgaagacgcg ttcaccgtca cgggcactct
    10681 caagttcttc gatcatcccg ccaatagcgg caatatcgtt ggccgcggtt tctgccccga
    10741 atgcggcgcg ccgatctatt cgcggaactc ggccatgaag ggcctcgtgt tcgtgcgcgc
    10801 ttccagtctc gatgatctgg agatcgccaa gccgcaggtc atcgtctttg cgagccgcgc
    10861 gccgtcgtgg cggcatctgg atccagagct cccggcctat gccgagatgc ctgagaagat
    10921 gccaaaggct gtgagcaagc ccgcaagttc ctaacagcga ttgcccgcgc gcaaagtctc
    10981 taatgcttga gccgactcgt tctcgtcttt aaacccattg catggcgctg agacgagcaa
    11041 gacttctggc tacggccaga cgttggacca ttccgaatct caagcacttc cgggaaagcc
    11101 ggcagccgac ggtttggctc ttggcgccgc tgatcgggct tgccacgggc gtcgccgcga
    11161 ttctcttccg ccttgccatc ggcgtcttcc agtggccgtg gctgggcacc atgtcggaga
    11221 atgtggccat ggccgcccgc gcgcagccgt ggtgggtgat catgcttgca cccgcgatcg
    11281 gggggctcct ggtcggactc ttgctgcgct acgtgttgac cgcgcgtcgc acggacgccg
    11341 tgcccgacgt catggaggcg cgagtcaatg cggggcgggg catgaagttc gggcaaggcc
    11401 tgctcagcgc gctcacaagt gcgttgtcgc tgggcgcagg cgcaagtgcg ggccgcgagg
    11461 gcccgatcgt ccatcttggc gcggcgcttg ccgcctgcgg ctccacgcac ttcaatctgc
    11521 ccaatgcctc gcggcgtacg ctgctggcgt caggcgccgc cggcgccgtc gccgcttcgt
    11581 tcaatgcgcc catcgccggc gtgctgttcg cccaagaggt catcctcggt catttttcga
    11641 ttaccacctt cgtgccgctg gtgctgtcgt cggtggtggc gacaatcatt tcccaggcct
    11701 ggttcggcga cgtggcggca ttcatcgttc cgagctaccg catcgtgtcc tatctcgaag
    11761 tgccggcctt cgtgctactc ggcatcgtgg cggcggccgt ggcggttatc ttccagctga
    11821 ccatcctctc ggtggactgg ctcgcgcgcc aagtcaaaat gccgcttgtc tcccggccca
    11881 tggttggcgg cctgcttatt ggcatcatgg ccatctggtt tccggaagtg ctgggcgtcg
    11941 gctacgacac cacggacgcg gcgctgaagg gacagcttgc gatccacgtg atgatcatcc
    12001 tcattgcggt caaaacgatt gccacggcga tcgcgctttc gtcgcgcttc gggggcggcg
    12061 tcttctcgcc tgcgctttat ctcggggcga tgacgggcgg cgcgttcggg ctcattgccg
    12121 gcggcatttt ccctgaagtg g
//
LOCUS       DBNHVU010000161        16706 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_876341, whole genome shotgun sequence.
ACCESSION   DBNHVU010000161 DBNHVU010000000
VERSION     DBNHVU010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 16706)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16706)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16706
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_876341"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="AC16Y7_06520"
     CDS             complement(<1..213)
                     /locus_tag="AC16Y7_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR5709070.1"
                     /translation="MSEPTRGSAFQDKAFLVLLVAVSLAFGWILLPFYGAILWAVILA
                     LVFAPVQRWLGAILPGRRNLAALLTLL"
     gene            398..628
                     /locus_tag="AC16Y7_06525"
     CDS             398..628
                     /locus_tag="AC16Y7_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709071.1"
                     /translation="MRKIAVLLTAAFALLLVTTLGFQADATIGSGTQGLPIAAKTFSP
                     LDRVSCRTRGPMCPLGYTRLCRPWRCWCTPCR"
     gene            866..2008
                     /gene="recA"
                     /locus_tag="AC16Y7_06530"
     CDS             866..2008
                     /gene="recA"
                     /locus_tag="AC16Y7_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR5709072.1"
                     /translation="MSNAALRLVEAGGDMDKEKALEAAISQIERSCGKGSIMRLGQNE
                     TVVETEAIPTGSLGLDIALGIGGLPRGRVVEIYGPESSGKTTLALHVVAEAQKKGGVC
                     AFVDAEHALDTIYAQKLGVDLENVLISQPDTGEQALEIADTLVRSGAVDVLVVDSVAA
                     LTPKAELEGEMGDFQMGAQARLMSQALRKLTGSIAKSKCMVIFINQIRMKIGVMFGSP
                     ETTSGGNALKFYASVRLDIRRIGAIKERDEVVGNQTRVKVVKNKVAPPFKQVEFDIMY
                     GEGVSKMGELIDLGVKAGIVEKSGSWFSYDSERIGQGRENSKTFLKEHPEMATKIEQA
                     IRQSAGLIGDQMLDGEIEAEAGDDTAAIAEESTVTAIAAGGRKKKA"
     gene            2294..5092
                     /gene="alaS"
                     /locus_tag="AC16Y7_06535"
     CDS             2294..5092
                     /gene="alaS"
                     /locus_tag="AC16Y7_06535"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HIR5709073.1"
                     /translation="MSAAKSPGGKSRTSGKSNAGAKQGKAHIVPTRANDIRTGFLDFF
                     AKNDHLVLPSAPLVPRHDPTLMFTNAGMVPFKNVFTGQEKAKSPRAASSQKCVRAGGK
                     HNDLDNVGFTARHHTFFEMLGNFSFGDYFKERAIALAWELLTKKFELPADKLLITVYV
                     DDEDAASLWKKISGFSDNKILRIAGSDNFWSMGDTGPCGPCSEIFYDHGAHLPGGPPG
                     SPDQDGDRFVEIWNLVFMQYEQREDGSRVDLPHPSIDTGMGLERIAAVMQGTHDNYAI
                     DLFKTLIAASVELTGVAAEGGNAPSHRIIADHLRASSFLIADGVLPSNEGRGYVLRRI
                     MRRAMRHAHLLGAKEPLLHRLAPTLIGEMGRAFPELSRGEDLIEETLKLEESRFRETL
                     ERGLRLLDEAVESLKPGDTLPGEVAFKLYDTYGFPYDLTEDALKAKNIGVDAEGFGVA
                     MEHQRAEARKSWAGSGEAATEQVWFELKEELGATEFLGYETETASGEIVALVKDGARV
                     DKLIEGDRGIVIVNQTPFYAQSGGQVGDQGTLSIGEDVVFDVEDTQKKLHGLFVHQGA
                     LQKGTLLRGQTVTLDVDHTRRTTTRAHHSATHLLHEALRQVLGQHVAQKGSLVEPGRL
                     RFDFSHPKPMTADEVAAVEDMANAMILQNAPVETHLMTPDEAIEQGALALFGEKYGEE
                     VRVVSMGVAPGSPKAGEAYSIELCGGTHVARTGDIGLLKITGEGAVAAGVRRVEALTG
                     EAARSFLDAQDGRVREAAELLKVSPEEMLERLAAVVDERRKLERQLADAKRELALRPE
                     GGDSAAGAAVRELGSVKLLARTVHGVSPKDLRGLVDEAKQQLGSGIVAVVGVSDEGKA
                     GLIVGVTKDLTGTYSAVDLVRAGAEALGGKGGGGRPDLAQAGGPDGARADAALEAIAA
                     AISGEAPPAAPAKRAGQA"
     gene            5096..6319
                     /locus_tag="AC16Y7_06540"
     CDS             5096..6319
                     /locus_tag="AC16Y7_06540"
                     /inference="COORDINATES: protein motif:HMM:NF012730.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-gated ion channel"
                     /protein_id="HIR5709074.1"
                     /translation="MATPAVWRRWRRKVHDILEVGGESHPAGYVVNGFIVTLIVLNAI
                     AFAAETVGDLAAEYGFWFRAFNIFSVIVFTIEYVLRVWSAVEIPMLSRLPPWQARARF
                     AMRPIMLIDLLAFAPWYVHWIYPIDLRVLRVFRLFRLLKLVRYSPALQTLGRVLVDES
                     RALLGALLIMMVLLLFASTGIYFLEREAQPDTFGSIPAAAWWALATLTTVGYGDVVPV
                     TPLGKVLGGVVMLLGLGMFALPVAIIATGFTQESNRHQFVVTWSMVSRVPLFATMNEA
                     EIAEITKLVYTRSYLPGVPIVRAGDAGESMFLLAGGEATVEMGRGRRTTLKEGDFFGE
                     MALLEHRRHKHDVIAKTRCRVYVLDSQAFARLSRRHPEIIRRIREVAEERAKADRARP
                     PRKVARKTKTKARRS"
     gene            complement(6334..7563)
                     /locus_tag="AC16Y7_06545"
     CDS             complement(6334..7563)
                     /locus_tag="AC16Y7_06545"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIR5709075.1"
                     /translation="MDKIKVQNPIADLHGDEMTRIIWDMIKEKLIEPYLDIELLPFDL
                     GIEHRDATDDQVTIDAAHAIQKHGVGVKCATITPDEARVEEFGLKKMWRSPNGTIRNI
                     LGGVIFREPIICKNVPRLVPGWTKPIVIGRHAYGDIYRAQDFLVPGKGKLTITFEGED
                     GETIHREVHDFEGSGIALGMFNLDSSIRDFARASMNYGLNRDMPVYLSTKNTILKAYD
                     GRFKDLFQEIYDAEFKETFEERGLTYEHRLIDDMVAAALKWSGGYIWACKNYDGDVQS
                     DTVAQGFGSLGLMTSVLMTPDGETVLAEAAHGTVTRHYRQHQEGRATSTNSTASIYAW
                     GGALKHRAKLDGNEALLDFAETLEKVVVDTIESGSMTKDLALLVGEEQAWLTTEGFID
                     KIGENLRAAMTKRVTAA"
     gene            7665..8489
                     /locus_tag="AC16Y7_06550"
     CDS             7665..8489
                     /locus_tag="AC16Y7_06550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019998470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HIR5709076.1"
                     /translation="MMEANAKTGKRVGQDDAASAAPAIVLVRPQLGENIGFAARAMGN
                     FGLRDLRLVAPRDGWPNEKARATAAVAGVIVDEAKVFDTVEDAVADLNYVIATTARPR
                     EMVKEVLSPETAAAALRAKRQASQRTGVLFGAERSGLDNDSIVLADAILTAPVSAAFA
                     SLSLPQAVLLFAYEWLKSESPSDLGRTTTYDGPAREGISAPDTQPATRAELYNLFEHL
                     EGALDDAGFLRPPEKRPSMVRSIRNMFHRMGATDQDVRTWRGIVSALTRPPTEPRR"
     gene            8574..9218
                     /locus_tag="AC16Y7_06555"
     CDS             8574..9218
                     /locus_tag="AC16Y7_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709077.1"
                     /translation="MRVAWFHLGGATCAAVFAFLTSAPLQAADQSGFTAVAAEELDKR
                     SKQPQKKGLSDTRVRVMLTYAFSLIPGETPGPNGKPVPVDKSDFNTFVIPSDDARQVI
                     RAATRSAYAEVCGLPQLAQANLKALLAQESKKDWTRQQMLMVEALHLFALSYFTGNAK
                     IEEGPETTAASGTEETDGGESAVTQAPPAPKCPPEQKQKVESAINAYVASVPAN"
     gene            9438..10055
                     /gene="rpsD"
                     /locus_tag="AC16Y7_06560"
     CDS             9438..10055
                     /gene="rpsD"
                     /locus_tag="AC16Y7_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR5709078.1"
                     /translation="MTKRIHAKHKIDRRLGENIWGRPKSPQNKREYGPGEHGQRRRGK
                     LSDYGQQLRAKQKLKGYYGNITEKQFKSIYHEASRMKGDTSERLIGLLERRLDALVYR
                     AKFVPTVFAARQLISHGHVKVNGRRVTIPSYRCKEGDVIELRDKSKDLGMVLEAAQSG
                     ERDVPDYVDVDHGKMVATFTRTPTLSDVPYPVMMEPNLVIEFYSR"
     gene            complement(10150..10509)
                     /gene="grxD"
                     /locus_tag="AC16Y7_06565"
     CDS             complement(10150..10509)
                     /gene="grxD"
                     /locus_tag="AC16Y7_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HIR5709079.1"
                     /translation="MSEQDVQAVQDWIGKQIAGRDVVLFMKGTKSMPQCGFSMQVAQI
                     LSHLGVDFADINVLDDMSVREGVKAYSNWPTIPQLYVKGEFVGGCDIVREMFQAGELQ
                     EMLKTKGIANNAQTATA"
     gene            complement(10530..10763)
                     /locus_tag="AC16Y7_06570"
     CDS             complement(10530..10763)
                     /locus_tag="AC16Y7_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006466620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HIR5709080.1"
                     /translation="MAMAAEEIERLVKERFPDARVEIEDLAGDGDHYAASIVSEAFRG
                     KSRVQQHQMVYDALQGNMGGALHALALKTSAPQ"
     gene            complement(10796..13015)
                     /gene="purL"
                     /locus_tag="AC16Y7_06575"
     CDS             complement(10796..13015)
                     /gene="purL"
                     /locus_tag="AC16Y7_06575"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HIR5709081.1"
                     /translation="MSGAARAAREAETLSDALIAEHGINAEEYARLLKELGREPTLTE
                     LGVFSVMWSEHCSYKSSRVWLKTLPTTGPQVIQGPGENAGVVDLGDDQAAVFKMESHN
                     HPSYIEPYQGAATGVGGIMRDVFTMGARPVANMNALRFGAPDHPKTRHLLSGVVSGIG
                     DYGNCMGVPTIGGETNFDARYNDNILVNAMCVGLVDANRIFTSAAKGVGLPVVYVGSK
                     TGRDGIHGATMASAEFDDASEEKRPTVQVGDPFTEKLLLEACLELMNEDAIVAIQDMG
                     AAGLTSSSVEMADKGGVGIDLNLDRVPCREEAMTAYEMLLSESQERMLMVLKPGAEPI
                     AKRIFTKWELDFAVIGETTDTGNFVVRHDGVVQADIPLPALAHAAPLYERPWVARDPL
                     PLIAPDDVKAPESILGALECLMGSAHLSSRRWIYEQYDHMVMADTVQRPGGDAGVVRV
                     HGTDKGLAVSCDVTPRYCAADPFEGAKQAVAECWRNITATGAKPLGITDCLNFGNPER
                     PEIMGEFVRAVEGMGEACTVLDFPVVSGNVSLYNETNGGAIPPTPAVGGVGLIADIAH
                     MAGMGLRQDGDVLLLLGTKTGHLGQSTYMQVMEDRLEGAPPPVDLAAEKCTGDLVRSL
                     IASDAVTAVHDVSDGGLLVAIAEMALAGNRGVQLEAPPEGLPAHAAWFGEDQARYVIA
                     VPADDVDAVGAAASAAGVPVRVLGTVEGDSIALPGETALALTALKAAHEGWMPRFMA"
     gene            complement(13012..13701)
                     /gene="purQ"
                     /locus_tag="AC16Y7_06580"
     CDS             complement(13012..13701)
                     /gene="purQ"
                     /locus_tag="AC16Y7_06580"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HIR5709082.1"
                     /translation="MKASVIVFPGSNCDRDAAVALQAAMGAAPAMVWHGDSELPKSDL
                     IVLPGGFSYGDYLRSGAMAAHSPVMREVVARAKAGTPVLGICNGFQVLTESGLLPGVL
                     MRNATLRFICKDMLLKVVRNDTRFASHYETGEVIRIPVAHKDGCYFADEGTLDRLEGE
                     NRVAFRYCEASGAVTEAANPNGSCRNIAGIYNETGTVLGMMPHPERLIEPVLSGTDGA
                     KMFSSLVERLH"
     gene            complement(13810..14055)
                     /gene="purS"
                     /locus_tag="AC16Y7_06585"
     CDS             complement(13810..14055)
                     /gene="purS"
                     /locus_tag="AC16Y7_06585"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HIR5709083.1"
                     /translation="MKARITITLKPGVLDPQGKAIQGALHALGFGSVDGVRQGKYIEV
                     EVAESDPARARAEVERMCEQLLANTIIENYAYELEAA"
     gene            complement(14073..14843)
                     /gene="purC"
                     /locus_tag="AC16Y7_06590"
     CDS             complement(14073..14843)
                     /gene="purC"
                     /locus_tag="AC16Y7_06590"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR5709084.1"
                     /translation="MNRRRRIYEGKAKILYEGPEPGTLIQHFKDDATAFNNKKHDRIE
                     GKGVLNNRISECIFERLNEIGVPTHFIKRLNMREQLIREVEIVPLEVVIRNVAAGSLA
                     TRLGLEEGTMLPRSIVEFYYKSDDLGDPMVSEEHITAFGWATPQELDDMMALALRIND
                     FLSGLFLGVGIRLVDFKLEFGRQWEADQMRIVLADEISPDCCRLWDIETQNKLDKDRF
                     RRDMGGLVEAYQEVARRLGLHVDNPGRPRSGPVLVQST"
     gene            15158..15487
                     /locus_tag="AC16Y7_06595"
     CDS             15158..15487
                     /locus_tag="AC16Y7_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008193764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HIR5709085.1"
                     /translation="MTSFDDREKSFERKFALDEELSFKANARRNKLLGLWAAEKMGLS
                     GDEAQAYAREVVKADLEEPGEEDVFRKIRGDFDAKSVDQSDHQIRRAMADHLAEAVRQ
                     IEAEAKA"
     gene            15495..16253
                     /locus_tag="AC16Y7_06600"
     CDS             15495..16253
                     /locus_tag="AC16Y7_06600"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR5709086.1"
                     /translation="MSRDLFRQAAKERQTLFNAWLMLNNPLALELAGEAGWDCVTLDQ
                     QHGAGGNETLLACLTAAKAAGLPAIVRVANNDPGLVGRALDAGAQGIMCPLINNVEEA
                     EAFVRAVKYPPRGLRSWGPYRAKIGYSGDYFTEANDFTIACPQVETKGALDQLDEILS
                     LEGVDMVCFGPNDLSVALTGRLDIWAKEVQEAMTEVLKKAREKSVMTLIFCNDADFAK
                     PRIEEGWDIVAVGTDAGWLSSAAEATRAAVSPGS"
     gene            16410..>16706
                     /locus_tag="AC16Y7_06605"
     CDS             16410..>16706
                     /locus_tag="AC16Y7_06605"
                     /inference="COORDINATES: protein motif:HMM:NF018095.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR5709087.1"
                     /translation="MGSFIRLVTGVLLGLVIATASASADDAQTAVSLNLRDGPGAGYA
                     RIAVVPAGALVDVRGCQGGWCGLIWNGYRGYAVQRGLLLASAYVLEPEVWPIFPA"
BASE COUNT         3065 a         5201 c         5402 g         3038 t
ORIGIN      
        1 cagaagcgtg agcagcgccg cgaggttccg gcgtcccggg agtatcgcgc ccagccagcg
       61 ctgcaccggc gcgaacacga gggccaggat cacggcccac aggatcgccc cgtagaacgg
      121 caacagaatc cagccgaacg ccaacgacac cgcgacgagc agaaccagaa aggctttgtc
      181 ttggaacgcg ctgccgcgcg tcggctcgct catattgacc agaatcgctc cctcaaggcc
      241 ccgatcatag cggcggcgtc tcgaacgtcg ccttgaaagt ttggccggtg aggagaaacg
      301 cagcaggggc caggggcgcg gttttgcgct atgttttccc cttggcgtcc ccgcgttcca
      361 gggggagggc ggtagggcgc ctttcaggga gggaacaatg cggaaaattg ctgttttgct
      421 gacggccgcc tttgccctgc tgctggtgac cacgctcggt ttccaggccg acgccacgat
      481 cggatcgggt acgcaagggc ttcccatcgc agccaagacc ttctcgccgc tggaccgggt
      541 ctcctgccgg accagggggc cgatgtgtcc gctgggctat acgcggctct gccggccctg
      601 gcgctgctgg tgtacgccct gccgctaggt tgagaggctg ggtagaagga atgctggccg
      661 ccccgtatcc gggcggcctt tattctttgc gcgaaaccgc gcttgcggcg atgagaacat
      721 attgcgaaca gagaacaaag cgtgtacact agttaaatgc tcgaccgacc cagtcgcctg
      781 cgtcatttgc ggtgtggacg ggaccggcgg gaccgagggc caccatttac gagccccgtg
      841 gggctgtgaa ataaggagat gaccgatgtc gaacgccgcg ttgcgccttg tcgaagcagg
      901 aggggacatg gacaaagaga aggcgctgga ggctgcgatc tcccagatcg agcgctcgtg
      961 cggcaagggg tcgatcatgc ggctcggcca gaacgagacc gtcgtggaga ccgaggccat
     1021 ccccaccggt tcgctcggcc tggatatcgc gcttggtatt ggcggcctgc cgcgcggccg
     1081 cgtcgtcgag atttacgggc ctgaatcatc gggcaagacg acgctggcgc tgcatgtggt
     1141 cgccgaggcc cagaagaagg gcggcgtgtg cgccttcgtc gatgcagagc atgcgctcga
     1201 caccatctat gcgcaaaaac tcggcgtcga tctggagaac gtactgatct cccagcccga
     1261 cacgggcgag caggcgttgg aaatcgccga cacgctggtg cgctccggcg ccgtcgacgt
     1321 gctggtggtc gattcggttg cggccctcac gccgaaggcc gagctcgaag gcgagatggg
     1381 cgattttcag atgggcgccc aggcgcggtt gatgagccag gcgctgcgta agctcaccgg
     1441 ctccatcgcc aagtccaagt gcatggtcat cttcatcaat cagatccgca tgaagatcgg
     1501 cgtgatgttc ggctcacccg aaaccaccag tggcggcaac gccttgaaat tctacgcctc
     1561 cgtccgtctc gacataaggc gcatcggcgc catcaaggag cgcgacgagg tggttggcaa
     1621 tcagacccgc gtcaaagtgg tgaagaacaa ggtcgcgccg cccttcaagc aggtcgagtt
     1681 cgacatcatg tatggcgagg gcgtgtccaa gatgggcgaa ctgatcgatc tcggggtcaa
     1741 agccggcatc gtcgagaagt ccggctcctg gttctcctac gatagcgagc gtattggcca
     1801 gggccgcgag aactccaaga cgtttctcaa ggagcatccg gagatggcca cgaaaatcga
     1861 gcaggccatc cgccagagcg cgggactcat cggcgatcag atgctcgacg gcgagattga
     1921 ggccgaggcg ggcgatgaca cggccgctat tgccgaggag agcaccgtga ccgccattgc
     1981 cgcgggcggc cggaagaaga aagcgtaaca cccccgccaa aggccccccg ctgggggcca
     2041 ggccgctgtg ggccggacga ggacaggaca agggcaggac gaaggcaggc gccgcgacat
     2101 cagcggcgcc tttgccattc ttgccgccgg gctggcctta ccggctgggc aggctctgcc
     2161 tgctggacag gccaaactcc agaccgtatg tctcacggcg aatgtagccg cgcgatgagg
     2221 cggccgccga atcggctagg gtagcggtgg cgagcgccgt ttcgcgcgag aacgaaggtc
     2281 ccagctgaac ctcatgagcg ccgcgaaatc cccgggcggg aagtccagaa ccagcggaaa
     2341 atccaacgcg ggcgccaagc agggcaaggc gcatattgtg ccgacgcgcg cgaacgacat
     2401 ccgaaccggt tttctcgatt tcttcgccaa gaacgaccac ctcgtcttgc cgtccgcgcc
     2461 gctggtgccc cggcacgatc cgaccttgat gttcaccaat gccggcatgg tgccgttcaa
     2521 gaacgtgttc accggccagg agaaggcgaa gagcccgcgc gcggcctcgt cgcaaaaatg
     2581 cgtgcgtgcc ggtggcaagc acaacgatct cgacaatgtg ggcttcaccg cgcggcatca
     2641 cactttcttc gagatgctcg gcaatttctc gtttggcgac tatttcaagg agcgcgccat
     2701 cgcgctcgcc tgggagcttc tgaccaagaa gtttgagctg ccggccgaca agctgctgat
     2761 caccgtctat gtcgacgacg aagacgcggc ctcgctgtgg aagaagatct cgggcttctc
     2821 cgacaacaag atcttgcgta tcgcggggtc ggataatttc tggtccatgg gcgataccgg
     2881 cccgtgcggg ccgtgttcgg agatcttcta tgaccacggc gcacatttac ccggcggtcc
     2941 gcccggcagt cccgatcaag acggcgaccg tttcgtggag atctggaatc tcgtcttcat
     3001 gcaatatgag cagcgcgagg acggctcgcg cgtggacctg ccgcatccgt ccatcgacac
     3061 cggtatgggg ctggagcgca tcgcagcggt gatgcagggc acgcacgaca attacgccat
     3121 tgatcttttc aaaacgctga tcgccgcttc tgttgagttg acgggcgtcg cggcggaggg
     3181 cggcaacgcc ccaagtcacc gcatcatcgc cgatcacttg cgtgcatctt catttctcat
     3241 cgccgacggc gtgctgccgt ccaatgaagg ccggggttat gtgctccgtc gcatcatgcg
     3301 ccgcgccatg cgtcacgcgc atctgctggg cgccaaggag ccgctgctgc accgtttggc
     3361 gccgacgctg atcggtgaga tgggtcgcgc ctttcccgag ctgtcacgcg gcgaggacct
     3421 catcgaggaa acgctgaagc ttgaggagag ccgcttccgc gagaccctcg aacgcggctt
     3481 gcgcctactg gatgaggcgg tcgaaagcct caagccgggc gacacgctgc cgggcgaggt
     3541 cgccttcaag ctctacgaca cctacggttt tccttacgat ctcaccgaag acgcgctaaa
     3601 ggccaagaac attggcgtgg acgccgaagg cttcggcgtg gccatggagc accagcgtgc
     3661 cgaggcgcgg aagtcttggg ccggctcggg cgaagcggcc accgaacaag tctggttcga
     3721 actgaaggag gaactcggcg ccaccgagtt tctcggctac gagaccgaga cggcctccgg
     3781 cgagatcgtg gcgttggtca aagacggtgc gcgtgtcgac aagcttatcg aaggcgaccg
     3841 tggcatcgtc atcgttaacc agacgccgtt ctatgcccag tccggcggtc aggtcggcga
     3901 tcaagggacg ctgtcgatcg gcgaagacgt cgtcttcgac gttgaggaca cgcagaagaa
     3961 gcttcatggc ctgttcgtgc atcagggcgc ccttcagaag ggcacgctgc tgcgtggaca
     4021 aaccgtgacg ctcgatgtcg atcatacgcg acgcaccacg acccgcgcgc accattcggc
     4081 cacgcatctt ctgcacgagg ccttgcgcca agtgctgggt cagcacgtgg cgcagaaggg
     4141 ctcgctggtg gaaccggggc gcttgcgctt cgatttctct catcccaagc ccatgacggc
     4201 ggatgaggtc gccgcggtcg aagacatggc gaacgccatg atcttgcaga acgcgcccgt
     4261 cgaaacacat ctgatgacgc ccgacgaggc gatcgaacag ggcgcgctgg cgctgttcgg
     4321 cgagaaatac ggcgaggagg tccgcgtggt ctccatgggc gttgcgcccg gaagccccaa
     4381 agcgggggag gcctattcca tcgagctgtg cggcggcacc catgtggcgc gcacaggcga
     4441 tatcgggctt ctcaagatca cgggcgaggg cgccgtggcg gccggcgtcc gccgcgtcga
     4501 agcgctgacc ggtgaggcgg cgcggagttt cctcgatgcg caggatggcc gcgtgcgcga
     4561 agcggcggag ctgttgaaag tttctccgga agagatgctt gagcgtcttg ccgcggtcgt
     4621 ggacgagcgc cgcaagctgg agcggcaact ggccgatgcc aagcgcgagc tcgcgctgcg
     4681 gcccgaaggc ggcgacagtg cggcgggtgc cgccgtgcgc gagctcgggt ccgtcaagct
     4741 actggcgcgc accgtgcacg gtgttagccc aaaggatttg cgcgggctgg tggacgaagc
     4801 caagcagcag ctgggctcag ggatcgttgc tgtggtcggc gttagcgacg aaggcaaggc
     4861 cggcctcatt gttggcgtca cgaaagacct caccggcaca tacagcgccg tagacctcgt
     4921 gcgcgctggt gccgaggcgc ttggcggcaa gggcggcggc gggcggcccg accttgccca
     4981 agccggcggc cccgacggcg cgcgcgccga tgcggccctt gaagcaatcg ccgcggcgat
     5041 ctccggcgag gcgcctcctg ccgcgccggc caaacgcgcc gggcaagcct aagcgatggc
     5101 gacgccggcc gtctggcgcc gctggcgcag gaaggtccac gacatcctcg aggttggcgg
     5161 ggagtcccat ccggcaggct atgtcgtcaa cggtttcatc gttacgctga tcgttctgaa
     5221 cgcgatcgcc ttcgcggccg agaccgtggg cgatctcgcc gccgagtacg gcttctggtt
     5281 ccgtgcgttc aacatcttct cggtgatcgt cttcacgatc gaatatgtgc ttcgggtgtg
     5341 gagcgccgtc gagattccca tgctgtcgcg tttgccgccc tggcaggcgc gggcgcgctt
     5401 cgccatgcgc ccgatcatgc tgatcgacct gctcgccttc gcgccgtggt acgtgcattg
     5461 gatctaccca atcgacctgc gcgtgctcag ggtgtttcgc ctgttccgtc tgctcaagct
     5521 ggtgcgctac tcgccggcct tgcagacctt ggggcgcgtg ctggtagacg aatcccgcgc
     5581 gctgctcggc gcattgctca tcatgatggt tctcctgctg ttcgcttcca cgggcatcta
     5641 cttcctggag cgggaggcgc agccggacac gttcgggtcc attccagccg ccgcttggtg
     5701 ggcgctcgcc acgctgacga cggtgggata tggcgacgtc gtgccggtca cgccgctggg
     5761 caaggtactg ggcggcgtgg tgatgctgct ggggcttggc atgttcgcac tgcccgtggc
     5821 gatcattgcc accgggttca ctcaagaatc gaaccgtcat cagttcgtgg tcacgtggtc
     5881 catggtgtcg cgcgtgccgc tgttcgccac catgaacgag gcggagatcg ccgagatcac
     5941 caagctggtc tatacgcgca gctatctgcc cggcgtgccc atcgtgcgag cgggcgatgc
     6001 gggcgagagc atgttcctct tggccggcgg cgaggccacg gtcgagatgg gccgcggccg
     6061 gcggaccacg ctgaaggagg gtgacttctt cggcgagatg gcgctgcttg agcatcggcg
     6121 ccacaaacac gacgtgatcg ccaagacccg atgccgcgtc tacgttctgg atagccaggc
     6181 cttcgcgcgg ctttcccgcc gtcatccgga aatcatccgc cgtatccgcg aagtcgcgga
     6241 ggagcgggcc aaagccgacc gagcacggcc cccgcgcaag gttgcgcgga agacgaagac
     6301 gaaagctcga agaagctgaa cggtggctgt ggcttaagcg gccgtcacgc gcttggtcat
     6361 cgccgcccgc aggttctcgc cgatcttatc gatgaaccct tccgtagtca gccaggcttg
     6421 ctcttcgccg acaaggagcg cgaggtcctt tgtcatggag ccgctctcga tcgtatcgac
     6481 aaccaccttc tccagcgttt cggcgaagtc gagcagcgcc tcgttaccgt cgagcttggc
     6541 gcggtgcttc agtgcgccgc cccaggcgta gatcgaagca gtcgagttcg tcgatgtcgc
     6601 acggccctct tggtgctgcc ggtagtggcg cgtcaccgtg ccgtgcgcgg cctccgccag
     6661 cacggtctcg ccgtcgggcg tcatcaacac gctcgtcatg agtccaagcg agccgaaacc
     6721 ttgcgccacg gtgtcggact gcacatcgcc gtcatagttc ttgcaggccc agatataccc
     6781 accggaccat ttgagcgccg cggcgaccat gtcgtcgatc agccgatgct cgtaagtgag
     6841 cccgcgttcc tcgaacgtct ccttgaactc ggcgtcgtag atctcctgga acagatcctt
     6901 gaagcggccg tcataggcct tgaggatcgt gttcttggtg gacaggtaca ccggcatgtc
     6961 gcggttgagg ccatagttca tggaggcgcg cgcgaagtcc cggatggaac tgtcgagatt
     7021 gaacatgccg agcgcgatgc cggagccttc aaagtcgtgg acctcacgat ggatcgtctc
     7081 gccgtcctcg ccctcgaagg taatggtcag cttgcccttg ccgggcacca ggaagtcttg
     7141 ggcccggtag atgtcgccat aggcgtgacg cccgatgacg attggcttgg tccagccagg
     7201 caccagccgc ggcacgttct tgcagatgat gggctcgcgg aagatcacgc cgccaaggat
     7261 gttgcggatc gtgccattgg gcgaccgcca catcttcttg aggccgaact cctccacgcg
     7321 cgcctcgtcc ggcgtgatcg tcgcgcattt gacgccgacg ccgtgcttct ggatggcgtg
     7381 ggccgcgtcg atcgtgacct gatcgtcggt ggcgtcacga tgctcgatgc caagatcgaa
     7441 ggggagaagc tcgatgtcga ggtagggctc gatgagcttt tccttgatca tgtcccagat
     7501 gatccgggtc atttcgtcgc cgtgaaggtc ggcaatcggg ttctgcactt tgatcttgtc
     7561 cacgcgcgcg tctccgctgg aaggaaaggg ttgagcctta ggagggccgc ttaaggcatt
     7621 tcgcccccat tgcgggagcc cgccccctat agcatcgtgc ggccatgatg gaagcaaatg
     7681 cgaaaaccgg caagcgcgtg ggccaagacg acgcggcgtc cgcggcgccc gccatcgtgc
     7741 tggtaaggcc gcaactcggc gagaatatcg gctttgccgc tcgcgccatg ggcaatttcg
     7801 ggcttcgcga tctgaggctc gtggccccaa gggatggctg gccaaacgag aaggcgcgcg
     7861 caaccgccgc cgtcgccggc gtcatcgtcg atgaggcgaa ggtgttcgac accgtcgaag
     7921 atgccgtcgc cgacctcaac tacgtgatcg ccaccaccgc gcggccccgc gagatggtga
     7981 aagaggtgct gagcccggaa acggcggcag ctgctttgcg cgccaagcga caagcctctc
     8041 agcggaccgg cgtgctgttc ggggccgagc gcagcgggct cgacaacgac tccatcgtgc
     8101 tggccgatgc gatcctgacc gcgccggtca gcgcagcgtt tgcttccttg agcctgcccc
     8161 aggccgtgct gctattcgcc tatgaatggc tgaagagcga aagcccctcc gatctcggcc
     8221 ggacaaccac ctatgacggg ccggcgcgcg aagggatctc cgcgccggac acacagcccg
     8281 cgacccgtgc ggaactctac aacctgtttg agcatctgga aggggcattg gacgacgccg
     8341 gattcttgcg gccgccggag aagcggccgt ccatggtgcg gtcgatccgc aacatgtttc
     8401 atcgcatggg cgccaccgac caggacgtcc gcacctggcg gggaattgtg tcagccttga
     8461 cgaggcctcc aacagagcca aggcgataag gtgtcatagt tccgcccggt ttctcctatt
     8521 gcgatgcccg tcaacaatgc tagcttccgg ctcgataagg agaggggcgt tcaatgagag
     8581 ttgcgtggtt tcatctgggc ggggccactt gtgctgcggt ctttgcgttt ctgacgagtg
     8641 ctccgctcca agcggccgat caatctggtt tcacggccgt ggcagccgaa gagctcgaca
     8701 agcggagcaa gcagccgcag aagaaggggc tgagcgatac gcgggtgcgc gtcatgctca
     8761 cttacgcctt ctcgctgatt ccgggggaga cgcccggacc taacggcaag cctgtcccag
     8821 tcgacaagtc cgacttcaac accttcgtga ttccaagcga cgacgcacgc caagtcattc
     8881 gcgcggccac tcgttccgcc tatgcggagg tctgcggctt gccgcagcta gcgcaggcga
     8941 atctgaaggc gctcttggcg caggaatcca agaaggactg gacgcgccag cagatgctga
     9001 tggtcgaggc gctgcacctc tttgcgctgt cctacttcac cggtaacgcc aagatcgagg
     9061 agggccctga gacgaccgcg gccagcggta ccgaggaaac cgacggcgga gaatcggctg
     9121 tgacgcaggc gccgccggcg ccgaaatgcc ctccggagca gaagcagaag gtcgaaagtg
     9181 ccattaacgc ctatgtggcc tcggtcccgg caaactaggg cggcccgggc ctgcctgtgg
     9241 ccctaggaaa tcgcggcaat tgacagtttc cggctattcg gagtaacaga acggtcaaac
     9301 cggagccctg aagggctttt ggggccgccg gacccgctct ggcgatccgg accactcaac
     9361 atccatggat ttttgcggca gacgcgggaa agcagaccgc ccgcgaaaag cctaatttgg
     9421 ctttggagtt tttacgaatg acgaaacgca ttcacgccaa gcacaagatc gaccgtcggc
     9481 tcggcgagaa tatttggggc cggcccaaga gcccccagaa caagcgcgaa tacggacctg
     9541 gcgagcatgg ccagcgccgc cgcggcaagc tgtccgatta cggccagcag ctcagggcca
     9601 agcagaagct caagggctat tacggcaaca tcaccgagaa gcagttcaag tcgatctatc
     9661 acgaggcctc gcgcatgaag ggcgacacct cggagcggct gattggcttg ctcgagcggc
     9721 gcctcgatgc gctggtctat cgcgccaagt tcgtgccgac agtgttcgcc gcccgccagc
     9781 tcatttccca cggccacgtg aaggtcaacg gccgccgcgt aacgatcccg agctatcgtt
     9841 gcaaggaagg ggatgtgatt gagttgcgcg acaagtccaa ggatcttggc atggtgctcg
     9901 aggccgcgca gagcggcgag cgcgacgtgc cggactatgt cgacgtcgac cacggcaaga
     9961 tggtcgcgac gttcacgcgg acgccgaccc tgtccgatgt gccctatccg gtcatgatgg
    10021 agccgaacct cgtcatcgag ttctactcgc gctaagcacg cgacgtcaga caagacaaca
    10081 cagcaatacg gcggcgattg aaacaatcgc cgccgtttgt gtatccagca gaaatcctaa
    10141 gaagcgcggt tatgccgtcg cggtctgcgc gttattggca atgcccttgg tcttgagcat
    10201 ttcctggagc tcgcctgcct ggaacatctc gcgcacgata tcgcacccgc cgacgaactc
    10261 gcctttcacg tagagctgcg gaatggtcgg ccagttggaa taggccttca cgccttcgcg
    10321 cacgctcatg tcgtcgagca cgttgatgtc tgcaaagtcg acgccgagat ggctcaggat
    10381 ctgcgcgacc tgcatggaga agccgcattg cggcatcgac ttggtgcctt tcatgaacag
    10441 caccacgtca cggccggcga tctgcttgcc gatccagtcc tgaacggcct gaacgtcttg
    10501 ctcactcatc gttcgattcc tttctcactc tattgcggcg ctgatgtctt caacgccagc
    10561 gcgtgcagtg cgccgcccat attgccctga agtgcatcat agaccatttg gtgctgctgg
    10621 acccgcgact tgccgcgaaa tgcctccgac acgatgctgg ccgcgtaatg atcgccgtcg
    10681 ccagcgagat cttcgatctc cacgcgcgca tcgggaaagc gttccttgac caagcgctca
    10741 atctcctccg cagccattgc catcgtcaat tgctccttgt gcgcgcggct tcaggttatg
    10801 ccatgaagcg cggcatccac ccctcatggg ccgccttcag tgcggtgagg gccagcgcgg
    10861 tctcgccggg aagcgcaatg ctatcaccct cgaccgtgcc gagcaccctt acgggaacgc
    10921 ccgcggcaga cgcggccgca ccgacggcat ccacatcgtc agccggtacc gcgatcacat
    10981 agcgtgcctg gtcctcgccg aaccaggctg cgtgcgccgg caagccctca ggcggcgctt
    11041 caagttgcac cccacggttg ccggcaagtg ccatctcggc gatggcgaca aggagcccgc
    11101 cgtcggagac gtcgtgcacc gccgtcacgg catccgacgc gataaggctg cgcaccagat
    11161 ccccggtgca tttctcggcg gcgagatcga cagggggtgg cgcgccttca agacgatctt
    11221 ccatgacttg catataggtc gattggccga gatgaccagt cttcgtgcca agcaggagca
    11281 gcacatcgcc gtcttgccgc aagcccatgc cggccatatg cgcgatatcg gcgataagcc
    11341 cgacgccgcc gacggcaggc gttggtggaa tagccccacc attggtctcg ttgtagagcg
    11401 acacgttgcc cgacacgacc gggaagtcca gcacggtgca cgcctcgccc atgccctcta
    11461 cggcgcggac gaactcgccc atgatttctg gccgctcggg attgccgaaa ttcaggcagt
    11521 ctgtgatgcc caacggtttg gcgcccgtcg cagtgatgtt gcgccagcat tcggccacag
    11581 cctgcttggc gccctcgaac ggatcggcgg cgcaataacg cggggtaacg tcgcaagaaa
    11641 ccgcaagccc cttgtcggtg ccgtgcacgc gcaccacgcc ggcatcgccg ccgggccgct
    11701 gtaccgtgtc ggccatgacc atgtggtcgt attgttcgta gatccagcgg cgcgacgaca
    11761 gatgcgccga tcccatcaag cattccagcg cgccaaggat gctctccggc gctttcacgt
    11821 cgtccggggc aatcagcggc agcggatcgc gcgcgaccca ggggcgttcg tagagcggag
    11881 cggcatgagc cagagcgggc aggggaatgt cggcctgcac cacgccgtca tgacgcacga
    11941 cgaaattgcc ggtgtcggtg gtctcgccga tcactgcaaa atcgagctcc cacttcgtaa
    12001 aaatgcgttt cgcaatgggt tcggcgccag gcttcaacac catgagcatg cgttcctggc
    12061 tctccgacag cagcatctcg taagctgtca tcgcctcttc gcggcatggc acgcggtcca
    12121 ggttaaggtc gatgccgacg ccgcccttgt cggccatttc gacggaagac gaggtgaggc
    12181 ccgcggcgcc catgtcctga atggcgacga tggcgtcctc gttcatcagc tcaagacaag
    12241 cttccagcag cagcttttcg gtgaaggggt cgccaacctg caccgtcggc cgcttctctt
    12301 ccgaggcatc gtcgaactct gccgacgcca tggtggcgcc gtgaatgccg tcgcggcccg
    12361 tcttcgagcc gacatagacg acgggaaggc cgacgccttt ggcggcggac gtgaagatgc
    12421 ggttcgcatc cacaaggccg acgcacatgg cgttgacaag aatgttgtcg ttgtagcggg
    12481 cgtcgaaatt cgtttcgccg ccgatcgtcg gcacgcccat acagttgccg tagtcgccga
    12541 tcccggagac gacgccggac aaaagatgcc gcgtcttggg atggtcgggc gcgccgaagc
    12601 gcagcgcatt catattggca acggggcgcg cgcccatggt gaacacgtcg cgcatgatac
    12661 cgcccacgcc cgtcgccgcg ccctgatagg gctcaatata ggaggggtgg ttatggctct
    12721 ccatcttgaa gaccgccgct tggtcgtcgc cgagatcgac gacgccggca ttctcgccgg
    12781 gcccttggat gacttgtggc cccgtcgtcg gcaatgtctt cagccagacg cgtgaggatt
    12841 tgtacgagca gtgctccgac cacatcaccg aaaacacgcc gagctccgtc aatgtcggtt
    12901 cgcggccaag ctctttcagg agccgcgcgt actcctcggc gtttatgccg tgctcggcga
    12961 tcagtgcatc ggaaagcgtt tccgcctcgc gggccgcccg cgccgcgccg ctcaatggag
    13021 cctctcaaca agcgaagaga acattttggc gccgtccgtg ccggagagca caggctcgat
    13081 gagccgttcg gggtggggca tcatgccgag caccgtaccg gtctcgttgt agattcctgc
    13141 gatatttcgg cacgagccat tggggttcgc cgcttcagtc accgcgcccg atgcctcgca
    13201 gtagcgaaag gcgacacggt tctcgccttc gagccggtca agcgtgccct cgtcggcgaa
    13261 gtagcagccg tctttatgcg cgacgggaat gcggatcact tcgccggtct cgtagtggga
    13321 cgcaaagcgc gtgtcgttgc gtaccacttt cagcagcatg tccttgcaga tgaagcgcaa
    13381 ggtcgcgttg cgcatcagaa cgcccggcag caggccggat tccgtcagca cctggaagcc
    13441 gttgcagatg ccgagcaccg gtgttcccgc tttagcgcgc gcgaccacct cgcgcatgac
    13501 cggcgaatgc gccgccatgg cgccggagcg cagataatcg ccataggaga agccgcccgg
    13561 cagaacgatg agatcgcttt tgggcagctc ggagtcgcca tgccagacca tggcaggcgc
    13621 cgcgcccatc gctgcctgaa gcgctacggc cgcgtcgcgg tcgcagttcg atcccggaaa
    13681 gacgatgacg ctggctttca tgttcgcacg aacccagacg tcatcccggc acaagccggg
    13741 atccagaagc ggcaattgtc cgagtgtgtg gttggattcc ggctttcgcc ggaatgacgg
    13801 agaatgcgcc tacgctgcct cgagctcata agcgtaattc tcgatgattg tgtttgcgag
    13861 aagttgttca cacatgcgct ccacttcggc gcgcgcgcgg gccggatcgc tttcggcgac
    13921 ttcgacctcg atatacttgc cctgacggac accatcaacc gagccgaagc cgagcgcatg
    13981 cagcgcgccc tggatcgcct tgccttgcgg atcgagcacg ccgggcttca gcgtgatggt
    14041 gatacgcgcc ttcatgccgc tttgcgctcc cgttaggtcg actgcaccag aaccggaccg
    14101 ctgcgcggcc gccccggatt gtcgacatgc agaccgagcc ggcgcgccac ctcttggtag
    14161 gcctccacga ggccgcccat gtcgcggcgg aaccgatcct tgtccagctt gttctgagtc
    14221 tcgatatccc acaagcggca gcagtcgggg gagatctcgt cggcgagcac gatgcgcatc
    14281 tgatcggcct cccactgccg gccgaactcg agcttgaagt ccacgagtcg gatgccgacg
    14341 ccaagaaaca gtcccgacag aaagtcgttg atgcgcagcg ccagcgccat catgtcgtcg
    14401 agctcctgag gcgtcgccca gccgaaagcg gtgatgtgct cttcggagac catggggtct
    14461 ccgaggtcgt cggacttgta atagaactcg acgatggagc gcggcagcat cgtgccttct
    14521 tcgagaccga gccgggttgc cagtgagccc gcggcgacgt tgcggatcac cacttcgagc
    14581 ggaacaatct cgacctcgcg gatcagctgc tcgcgcatgt tcagccgttt gatgaaatgc
    14641 gtcggcacgc cgatctcgtt gagccgctcg aagatgcact ccgaaatgcg attattgagt
    14701 acgcctttgc cctcgatgcg gtcgtgcttc ttattgttga aggcggtggc gtcgtccttg
    14761 aagtgttgga tgagcgtgcc aggttcgggt ccttcgtaga ggatcttggc cttgccttcg
    14821 tagatgcgcc ggcgacgatt cataattttg gttcctcact gccctgtctg gggactcaag
    14881 cgccaaacgc gcgaagactg ggaaagtccc aagccgcgcg cgctacactg gaaattgcca
    14941 tcgcaggaat cggattcaag aagtcttatt cgacattagc tgaaggggag ggggcgtaca
    15001 acggctccgg gatgggcctt ccggccgcag gggacggccg gggatttgtt gattttccgg
    15061 cgcttcggcg ctatcacgcg gtccagagag gcgggatttt ccccaacaat tctatggacg
    15121 atgccctcgg tcgccgaggg tcctggaggt gccggcaatg accagttttg acgatcgcga
    15181 gaaaagtttt gagcggaaat tcgccctcga cgaggagttg agtttcaagg ccaatgccag
    15241 gcggaacaag ctcttgggcc tatgggccgc cgagaaaatg ggcctgtccg gcgatgaggc
    15301 ccaggcctac gcccgggagg tggtcaaggc cgacctggag gagccgggcg aggaggacgt
    15361 cttccgcaag atcaggggcg atttcgacgc caaaagcgtc gatcagtccg accaccagat
    15421 tcgccgcgcc atggccgacc atctggccga ggcggtgcgg cagatcgaag ccgaggccaa
    15481 ggcgtaagga caccatgtcc cgggacctgt tccgccaagc tgccaaagag cgccagacgc
    15541 tgttcaacgc ctggctgatg ttgaacaacc cgcttgctct cgagctggca ggcgaggccg
    15601 gctgggactg tgtcaccctc gaccagcaac atggcgcagg gggcaacgag acgttgctgg
    15661 cctgcctgac cgccgccaag gcagccgggc tgcccgctat cgtgcgcgtg gccaacaacg
    15721 atcccgggct cgtgggccgg gcgctggatg cgggcgcgca aggcattatg tgcccgctca
    15781 tcaataacgt ggaggaggcg gaggccttcg tccgggccgt gaagtatccg ccgcggggct
    15841 tgcgcagctg ggggccgtac cgggccaaga tcggctacag cggcgactat ttcacggagg
    15901 cgaatgattt caccatcgct tgtccgcagg tcgagaccaa aggcgccctc gatcaactcg
    15961 acgagatact gtcgttggag ggcgtggaca tggtctgctt cgggcccaac gacctctccg
    16021 tggcgctcac cgggcgcctc gatatctggg ccaaagaggt tcaagaggcc atgacggagg
    16081 tcctgaaaaa ggcccgcgag aaatccgtca tgaccctgat tttctgcaat gacgcggact
    16141 tcgccaagcc ccggatcgaa gagggctggg acatcgttgc cgtcggcacc gatgccggct
    16201 ggctgtcgtc cgccgctgag gcgacccgcg cggcggtttc gccgggctct taagctcggc
    16261 accgcaacgc cttcaaacga ttcgcgcatt ttagagcgtt gcatccttgc atcaggtcgg
    16321 gacccttagc cgatccggac gcaaggagag gctgacggtg ccgaggtcct atgcgacact
    16381 ggcccttaga gttgaatttt gggggcggta tgggatcctt catccggctc gtcacgggcg
    16441 tccttctagg gctcgtgatc gcgacggcat cggcgtcggc ggacgacgcg cagacggcgg
    16501 tctcgctgaa cttgcgcgac gggccgggcg ccggatatgc gcgcatcgcc gtcgtgcccg
    16561 cgggcgcgct ggtcgatgtt cgaggctgcc agggcggctg gtgcggtctg atctggaacg
    16621 gctatcgcgg ctatgcggtt cagcgcggac tgctgttggc ctcggcctac gttctggagc
    16681 cggaagtctg gccgatcttc ccggct
//
LOCUS       DBNHVU010000162         4514 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1618794, whole genome shotgun sequence.
ACCESSION   DBNHVU010000162 DBNHVU010000000
VERSION     DBNHVU010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1618794"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="AC16Y7_06610"
     CDS             complement(<1..997)
                     /locus_tag="AC16Y7_06610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03808 family TAT-translocated repetitive
                     protein"
                     /protein_id="HIR5709088.1"
                     /translation="MKIARREFMLAGLGAGLTAAADPHSAAARDTSAPEAFSTFGIVP
                     ADGAINQTATLQRAADAAAAAGTPLYLPAGTYATERLVLKTGTHIHGVPGLTILRHAG
                     GRAILETEGTDNVRLSGLVLDGDGKPLGDNGALIIATETTHLDIADCRVTGSSEEGIA
                     LRKAAGRVAECEIDTIAKGALVSEDANGLEISHNHVHDCGDNGILVWRSKTGEDGTIV
                     SANRIERIGAKSGGSGQNGNGINVFRAGSVLITQNRIADCAFSAIRSNAGSNCQMVAN
                     SCTRLGEVALYAEFGFQGAVVANNIVDTAAMGISVTNFNEGGRLAVVQGNVIRNLF"
     gene            complement(994..1599)
                     /locus_tag="AC16Y7_06615"
     CDS             complement(994..1599)
                     /locus_tag="AC16Y7_06615"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: protein motif:HMM:NF013624.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HIR5709089.1"
                     /translation="MAPRVLLTGFGPFPGVDENPSGWLAETLTARTPEGGLDLHGQIL
                     PTEWDAVAARTPQLYADLEPDVMIHFGVSQTAKALRIEHSAHNRALRRADARGALPPK
                     SAISPNGQPRLDSGLPVNALAAHLRAQGHGANASRSCGRYLCNYLYYRSLEWARERGR
                     DTIFIHVPLMRAQGGDLEEKNLLSAAQEALRFVVEAREARQ"
     gene            complement(1655..2671)
                     /gene="meaB"
                     /locus_tag="AC16Y7_06620"
     CDS             complement(1655..2671)
                     /gene="meaB"
                     /locus_tag="AC16Y7_06620"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HIR5709090.1"
                     /translation="MSGKKSTQTVKDLSKAIRAGDRVALGRAITLIESARPEDQARAR
                     DLLKTLLPDTGKAIRVGISGVPGAGKSTLIDQLGVNLISQGHKVAVLAVDPTSSRTGG
                     SILGDKTRMGQLASEDDAYIRPSPAGASLGGVTKTMRETISIAEAAGFDVVLVETVGV
                     GQSETAVSNMVDVYVVVAIPGAGDELQGIKRGLLELADIIAVNKADNDNIERANRAAT
                     EYRAALHILAASSAAWQPAVLTLSARDNTGLDALWDKIGECHSTLSEAGILDERRAEQ
                     ATAWMREIFEQRLLAAFKGGRRAARQYQDIEEQVRNGTLTPTEGAEALAELVGLNEPE
                     KEQE"
     gene            complement(2724..>4514)
                     /gene="scpA"
                     /locus_tag="AC16Y7_06625"
     CDS             complement(2724..>4514)
                     /gene="scpA"
                     /locus_tag="AC16Y7_06625"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008191620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HIR5709091.1"
                     /translation="VGMAGVAIDSIYDMRTLFDGIPLDQVSVSMTMNGAVLPVLALFI
                     VAGEEQGVAHKDLSGTIQNDILKEFMVRNTYIYPPEPSMRIVSDIIGYTSKEMPRFNS
                     ISISGYHMQEAGATADLELAYTLADGIEYVRAAIASGLDVDTFAPRLSFFWAIGMNFY
                     MEIAKMRAARLLWAKLMMDEFAPKNPKSLALRTHCQTSGWSLTAQDVYNNVIRTCVEA
                     MAATQGHTQSLHTNALDEALALPTDFSARIARNTQLMLQKESGTTRIIDPWGGSYYVE
                     RLTYDLAARAMQHIEEVEKFGGMAKAIEAGIPKRMIEQAATATQGRIDSGRQTIVGVN
                     KYRAEKDADIAILKVDNRAVRRQQLEKLERLRGERDEAAVREALDALTQYAETGTGNL
                     LEGAVAAARVKATVGEISDALEKIYGRHRANVSAITGVYKSEMGRSEDGNMARVAELI
                     DVFETEHGRRPRILVAKMGQDGHDRGQKVIASAFADLGFDVDIGPLFQTPEEAARQAV
                     ENDVHILGVSSLAAGHLTLVPALREALEAQGRGDIVIVLGGVIPPQDYPELYEAGAKA
                     IFGPGTPIADAAIDILAKLGIDAAKADAAE"
BASE COUNT          721 a         1387 c         1526 g          880 t
ORIGIN      
        1 agaacagatt gcggatcaca ttgccttgca ccacagcgag ccggccacct tcgttgaagt
       61 tggtgacgga gatgcccata gccgcggtgt cgacgatgtt gttggcgacc acggcgcctt
      121 ggaatccgaa ttcggcatag agcgccacct caccgagccg ggtgcaggaa ttggccacca
      181 tctggcagtt cgagccggcg ttggagcgga tggcggaaaa ggcgcaatcg gcgatgcggt
      241 tctgcgtgat cagcacagag cccgcacgga acacattgat accattgccg ttctgtccgc
      301 tgccgccgct cttggcgcca atgcgctcga tgcggttggc actgacgatt gtcccgtcct
      361 cgcctgtctt ggaccgccaa acgagaatgc cgttgtcgcc gcagtcgtgg acgtggttgt
      421 ggctgatctc gaggccgttc gcgtcttcgc tgacgagcgc acctttggcg atcgtgtcga
      481 tctcgcactc ggcgacgcgc ccagcggcct tgcgcagcgc gatgccctct tcgctcgagc
      541 ccgtgacgcg gcagtccgca atgtcgaggt gcgtcgtctc ggtggcgatg atcagcgcgc
      601 cgttgtcgcc aaggggcttg ccgtcgccgt cgaggacgag gcccgacagg cgcacattgt
      661 ccgtgccttc ggtctccaga atggcccggc cgcctgcatg gcggagaatc gtgagccccg
      721 gcacaccatg aatatgcgtg ccagttttta ggacaagccg ttctgtcgcg tatgtaccgg
      781 cgggaagata gagcggtgtg ccggccgcag cggctgcgtc ggcggctcgt tgtagcgtcg
      841 cggtctgatt gatggcgccg tccgcgggca caatgccgaa cgtgctgaag gcttccggcg
      901 cgctcgtgtc gcgagcggcc gcgctatgcg ggtcagctgc cgcggtaagc ccggcgccaa
      961 ggccggccag catgaactct cggcgggcaa tcttcattgc cgtgcttccc tggcttcgac
     1021 gacgaagcgc agcgcttctt gcgcggcgct caggagattt ttctcctcga ggtcgccgcc
     1081 ttgcgcgcgc atcaacggca catggataaa gatcgtgtcg cggccgcgct cgcgcgccca
     1141 ttcgagcgaa cggtaataga gatagttgca gagatagcgg ccgcaagagc gcgaggcgtt
     1201 cgcgccgtgg ccttgggcac gcaagtgtgc agcgagcgcg tttacgggaa gcccgctatc
     1261 gaggcggggt tgaccgttcg gcgaaatggc ggacttgggt ggaagggccc cacgcgcatc
     1321 ggcccggcgc agggcgcggt tatgcgcgct gtgctcgatg cgaagggcct tcgcggtctg
     1381 gctcacgcca aagtgaatca taacatcggg ttccaggtcg gcgtagagct gcggggtgcg
     1441 cgcggccacg gcgtcccatt cggtcggcag gatctggcca tggaggtcca aaccgccttc
     1501 cggtgtccgt gcggtaagcg tttcggcgag ccagcccgag gggttctcgt cgacgccggg
     1561 aaagggacca aagcccgtga ggaggacgcg cggtgccata tcaatggctt actaggggct
     1621 tgggcgcgag ggaaggaaat tcgcgtgagc gtggttactc ttgttctttc tcgggctcgt
     1681 tcaaccccac gagctcggcc agggcctcag ccccttctgt cggggtcagc gtgccgttgc
     1741 gcacctgctc ctcaatgtcc tggtattgcc gcgccgcgcg gcggccgccc ttaaaagcgg
     1801 ccagcagacg ctgttcaaag atctcgcgca tccaggccgt ggcctgctcg gcgcggcgct
     1861 catcgagaat gcccgcttcc gacaacgtgc tgtggcattc gccgatcttg tcccacagcg
     1921 cgtcgaggcc ggtgttgtcg cgggcggaaa gtgtcagcac tgcgggctgc catgccgcgc
     1981 tgctcgcggc gaggatatgc agagccgcac ggtactcggt ggcggcgcgg ttggcgcgtt
     2041 cgatattgtc gttgtcggcc ttgttgacgg cgatgatgtc ggccagttca aggaggcccc
     2101 gcttgatgcc ttgcagctca tcgcccgcgc ctggaatggc aacgacgacg tagacgtcca
     2161 ccatgttgga tacggcggtt tcggattggc cgacgcccac cgtttcgacc aggacgacgt
     2221 cgaaacctgc tgcttcggca atggagatgg tctcgcgcat ggtcttggtc accccgccga
     2281 ggctcgcgcc ggcgggcgag gggcggatat aggcatcgtc ctcggaggcg agctgtccca
     2341 tgcgcgtctt gtcgccgaga atggagccgc cggtgcgcga ggacgtgggg tccaccgcaa
     2401 gcacagccac cttgtggcct tggctaatca ggttgacgcc gagctggtcg atgagggtcg
     2461 acttgccggc gccgggtacg ccgctgatac cgacgcggat cgccttgccc gtgtcaggca
     2521 acagcgtctt cagaagatcg cgcgcacggg cctggtcctc gggccgcgcg ctttcgatca
     2581 gggtgatggc gcgccccagg gccacgcggt cgcccgcgcg gatggccttg gagaggtcct
     2641 tgacggtttg agtgcttttc ttgccgctca tgacatgcgg gggtaggggc taacgggccg
     2701 aagcgcaagc tgcgatcgtc gcgtcactcc gcggcgtcgg ccttggcggc atcgatgccg
     2761 agcttggcga ggatatcaat ggcagcatcg gcgatgggcg tgcccggacc gaaaatggcc
     2821 ttggcaccgg cttcatagag ttctggatag tcctgaggcg ggatcacgcc gcccagcacg
     2881 atcacgatgt cgccgcgccc ttgcgcttcc aacgcctcgc gcaatgccgg caccagcgtc
     2941 aggtggccgg ctgccagcga cgatacgccg agaatgtgca cgtcgttttc gactgcctgg
     3001 cgcgcggcct cttccggcgt ttggaacaga ggtccgatgt cgacgtcgaa gccaagatcg
     3061 gcgaaggccg aggcgatgac cttctggccg cgatcgtgcc cgtcctggcc catcttggcg
     3121 accaggatgc ggggcctgcg gccatgctcg gtctcgaaaa cgtcgatcag ttctgcaacg
     3181 cgggccatgt ttccgtcctc gctgcgaccc atctcggatt tatagacgcc ggtgattgcc
     3241 gaaacgttgg cgcgatggcg gccatagatc ttctccagcg cgtcggagat ctcgccgacg
     3301 gtggccttga cgcgggccgc cgccacggcg ccctccagaa ggtttcccgt gcctgtctcg
     3361 gcgtattgcg tgagcgcgtc aagggcttcg cgtacggccg cttcgtcccg ctcgccccgc
     3421 agacgctcca gcttctccag ctgctgacgt cgcacggcac gattgtccac tttgagaatg
     3481 gcgatgtccg cgtccttttc ggcgcggtac ttgttcacgc cgacgatggt ctgccggccg
     3541 gaatcgatgc ggccttgcgt ggcggtcgcc gcctgctcga tcatgcgctt ggggatgccg
     3601 gcctcgatag ccttggccat gccgccgaat ttttcgacct cttcgatgtg ctgcatcgct
     3661 ctggccgcga gatcgtaggt cagccgctcc acgtaatagc tgccgcccca cgggtcgatg
     3721 atgcgcgtgg tgccgctttc cttttgcagc atgagctggg tgttacgggc gatccgcgct
     3781 gagaagtccg tgggcagagc cagcgcctcg tcaagcgcat tggtgtgcag cgactgcgta
     3841 tggccttgcg tcgccgccat ggcctccaca caggtgcgga tcacgttgtt gtagacgtcc
     3901 tgcgccgtga gactccagcc gcttgtctgg cagtgcgtgc gcagcgccag cgacttggga
     3961 ttcttcggcg cgaactcatc catcatgagt ttcgcccaga gcagacgcgc ggcgcgcatc
     4021 ttggcgatct ccatgtagaa gttcatgccg atggcccaga agaaggagag ccggggcgcg
     4081 aaggtatcga cgtcgagccc tgaggcgatt gccgcgcgca catattcgat gccgtcggca
     4141 agtgtgtaag cgagctcaag gtccgccgtc gcgccggctt cttgcatgtg atagccggag
     4201 atcgagatcg agttgaagcg cggcatctcc ttcgacgtgt agccgatgat atcggagacg
     4261 atgcgcatcg aaggctcggg cggatagatg taggtgttcc gcaccatgaa ctctttgagg
     4321 atgtcgttct gaatggtgcc cgagaggtcc ttatgggcga caccttgctc ttcgccggcg
     4381 acgataaaga gcgcgagcac cggaagcacc gcgccgttca tggtcatgga cacagagacc
     4441 tgatcgagcg ggataccgtc gaacagggtg cgcatgtcgt agatggagtc gatggcgacg
     4501 cccgccatgc cgac
//
LOCUS       DBNHVU010000163        13152 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1100094, whole genome shotgun sequence.
ACCESSION   DBNHVU010000163 DBNHVU010000000
VERSION     DBNHVU010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 13152)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13152)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13152
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1100094"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1659)
                     /locus_tag="AC16Y7_06630"
     CDS             complement(94..1659)
                     /locus_tag="AC16Y7_06630"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase scaffold domain-containing
                     protein"
                     /protein_id="HIR5709092.1"
                     /translation="MRGSCLVPVFLCAAQVLTFAVMRSACAEDPVAPPAVRSTPSTSK
                     APTPSTPAPQAVVAAIGAKLADSDLRKGVAADDLAALEAFYDDRSSVPLWTTDMGLSA
                     RAQQALFEIEKADDWGLDKHAFDLPPASALPTGPEEQAIAEIKLNLAILRYARFARGA
                     RSDPTALGGKVFQSPSMLDPKTVLADIAGSETPDAYLRSLHPQHTQFARLRQTLLVAR
                     DGDAAGDSRTDVKRIIMNMERWRWMPEDLGRVYVWSNTPAFMLYVVKDGQTIYADKTQ
                     VGTIDYATPVLSADMTTVVFNPEWIAPPTVLRENLAPALRKKNYSILKKKGFSVSYQG
                     KRVDPTKIDWRRVNILNYTFTQRPGPHSNIGKVKFLLPNRHAFVLHDTWPARRKYYQQ
                     AVRAIGHDCVRMEKPDRFAKVLLAESNGWPESKVTDLWDNSINNRVTLDSEIPVYSTY
                     FTAVADEQGKVTTFPDLYGLDHKLARALFGDAEGFPMPTRKGKRRRAVSKSVRWKPQG
                     SDVTRSLQGFPGR"
     gene            2121..2255
                     /locus_tag="AC16Y7_06635"
     CDS             2121..2255
                     /locus_tag="AC16Y7_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709093.1"
                     /translation="MKMLAVVLTALIAFVLFQSYRHDCHWRGQNHWAQWSACILDVPG
                     "
     gene            complement(2699..2887)
                     /locus_tag="AC16Y7_06640"
     CDS             complement(2699..2887)
                     /locus_tag="AC16Y7_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709094.1"
                     /translation="MNQQLMAGLMAVTLVVMIGATVAKDFTEARGRPSIWLDPHASDG
                     LLASRLNITSIAKTPLTR"
     gene            complement(3001..3396)
                     /locus_tag="AC16Y7_06645"
     CDS             complement(3001..3396)
                     /locus_tag="AC16Y7_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR5709095.1"
                     /translation="MSNSLQTVWGPRVLSILRIVSGLIFFAHGSAKLFNFPAANFTPE
                     AMSLPWIAGVLEVVGGALLIVGLFTRPVAFVLSGLMAAAYWIAHAPKSVYPLLNGGDA
                     AILYCFIFLYIACVGGGPWSLDALLRKKD"
     gene            3563..3760
                     /locus_tag="AC16Y7_06650"
     CDS             3563..3760
                     /locus_tag="AC16Y7_06650"
                     /inference="COORDINATES: protein motif:HMM:NF022384.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2735 domain-containing protein"
                     /protein_id="HIR5709096.1"
                     /translation="MTANAIGRTAKIYQFPEGGRAGLSSGRHEATDIEAVASKRTALV
                     ACDGASYHEDAIREEQHVREN"
     gene            complement(3839..5029)
                     /locus_tag="AC16Y7_06655"
     CDS             complement(3839..5029)
                     /locus_tag="AC16Y7_06655"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HIR5709097.1"
                     /translation="MDVYPSAAKLLSHHRPERPVLALRPHTVARAAAWFLANFPGRVL
                     YAAKANDAPPIIDALADAGIRAFDVASLVEIERVAGVEDAELYFMNPIKSRGAISRAY
                     REFGVRRFAFDSDEELDKVLDETNGADDLTLYLRIACPNTHSLIPLEGKYGASQEEAP
                     ALLLKAHQCAMRLGITFHVGSQAVVPAAFGEVLRQVGQLIVSSGVLVDAVDIGGGFPA
                     RYPHADPPALESFMEEIVRAADELAVKHSCELLCEPGRALVAEAESVIVRVDARRGDT
                     LYINDGAFGSLFDAAYSGFRFPARLVTPGKESAEPDTAFALYGPTCDSSDYLPGPFVL
                     PACVGEGDYLEIGQVGAYGRVLANRFNGFGTFDEVVLTDEPMLPMFAPGGDESSDTAW
                     PARA"
     gene            complement(5105..6772)
                     /locus_tag="AC16Y7_06660"
     CDS             complement(5105..6772)
                     /locus_tag="AC16Y7_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HIR5709098.1"
                     /translation="MAGRRGRGRGLIIGLALAVVGVAGAEARPTDMSVVAVVTPESEP
                     TPAPAELHDPKAKATARVQRCYAKLGYYKGAITGKANEATWTAHWHFKNDHGLKKHGD
                     FLSEAVQKKIKKLCKDVEPRPEPVEVKAPEPPAPNSPEEFENADTDTVTAALAPNAEP
                     VVAEELPPPPTRLEIDCLPEDLIAVLRKAHGEGVRAPACRRGCLPSPKGLSQSQLDDL
                     QARYGVGWCRSCLQIEGRLSLEDIRRIEDEGNLELCTVPPRQLPRPGSANDNVVKSFS
                     RIRELYRALPPAADDPGAIAVIIGNGKYDNLPPAETARNDAGAMYFFLTEYMGFRQDR
                     IIDLRNARLADFERVFGPVSGSDGELARLVRAQPNAKVVVYYSGHGATNSSHSETYLL
                     PVDAEPYREERGGYPLSTLYDNLAELKADKVLVLLESAYGRDHGAYVLPPNLPETTSS
                     ALPSAPLSNVTVLSGTDRGQRALIDPRYDVGLFTRYVIEGLAGRGDLYPVGNGDGVVD
                     SAELYAFVAAMARLSARKTFGLLQEPVYSSAATPALSGTGVADAKSN"
     gene            complement(6954..9224)
                     /locus_tag="AC16Y7_06665"
     CDS             complement(6954..9224)
                     /locus_tag="AC16Y7_06665"
                     /inference="COORDINATES: protein motif:HMM:NF025684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HIR5709099.1"
                     /translation="MRHRLVALAMLSCMAIGFGHAHAETSANMSKPQDGVARAAFDAL
                     DKHCARCHQAGPSLKRQKPAKNFGNVLKLTEIARDPDLILPGNPDGSSLFIQIAKKDM
                     PYDCYQEFDCKADPTEADVKAIYDWIRSLGDTVGAACGNRKPIGEAVVVRAIAKDIKA
                     QEMRRRPGMRYVTLTHFYNACASGEDMTRYRQGVVKLLNSLSRNPTPLTMRTIDPHAT
                     IIAFNLADLNWTTEDWARLAGLYPYGMAPSVPAYRDVVAATGAELPWLRGDWLAFIAS
                     RPPLYYDLLKLPTTFAELERQEGVDVKANIEAYLARRAGFQKSGVSKQNRLIERHAIP
                     TGYFWTSYDFKGDAPAQSLFVHPLGPFGDGAFRHDGGETIFSLPNGFQAYYLNTAEGE
                     RLGKGPTEIVLDDSQRDRAVTNAISCFGCHNQGIRQATDDIRAHVLRDRSFPEGVRKT
                     VDALYAPQDEMKALLTQDAEKFRKAMLAAGLDPELDSQTQGVESINFLSKAYEKSVDL
                     RIAAAEYGIEAEALAQGLADAGGEAVRIKRRLEQGVLPRQILEAEFPDLVAKVSGNAP
                     IRLAALTPTAVELIQDENEVFFDDFDLALTSDKSTYRVNDVAVFSVTSSKGCHLTLIN
                     VEAGGDGTVIFPNQFQQDNFLPANTEAQFPGDDAPFQYRLKDPGTETLIAICNATGVE
                     ADGIEHDFKTRQFTPVGNYREFLTRRIVVEGTPKVAAQGQPARPAKTQHEAPATPKDD
                     DASEDLARTAIKLVVR"
     gene            complement(9313..10281)
                     /locus_tag="AC16Y7_06670"
     CDS             complement(9313..10281)
                     /locus_tag="AC16Y7_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5709100.1"
                     /translation="MRRALLFLVGLIAIGAIGFYGLTMPVTVPASALPNHTSVIDNGK
                     VMFIAGGCAECHAKPIGKCDDLDIEDETVLVGGRCLNTDVGTFYVPNISPDAETGIGN
                     WTTLDFVSAMKRGVAPGGVHLYPSFPYTSYQRMTYADLIDLKAYLDTLPAVRNETPAH
                     TLRFPYNIRRGLGLFQRLYVDGKTFAPDPAVSDEVNRGAYLVRGPGHCGECHSPRNAF
                     GGIIADEAFAGAKNPDGKGTIPNITPSADGIGDWSEEDIVYLLETGSTPDFDVIGDTM
                     APVQENMEKLSAKDREAIAAFLKTLPSRPNAVQKSKTNADDEDDQS"
     gene            complement(10380..11012)
                     /locus_tag="AC16Y7_06675"
     CDS             complement(10380..11012)
                     /locus_tag="AC16Y7_06675"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR5709101.1"
                     /translation="MIDLYTWMTPNGQKVSIMLEEVGLPYRVHPVDIENGGAQFEPEF
                     TKISPNNKIPAIVDHDNELTLMESGAILMYLAEKTGKLWSHDFPTKWRVLEWLMWQKG
                     GVGPFLGQVQHFVKVSPGKSTYAEERYSKESKRLWSVLERRLGEVEYVAGDYSIADIA
                     IWPWISRFEWQTVDFDEHPNVKRWYVAIANRPAVQRGWDVLEVGRVMPMP"
     gene            11216..11956
                     /locus_tag="AC16Y7_06680"
     CDS             11216..11956
                     /locus_tag="AC16Y7_06680"
                     /inference="COORDINATES: protein motif:HMM:NF018053.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1045 domain-containing protein"
                     /protein_id="HIR5709102.1"
                     /translation="MFNCAHVDNHIREDAASNRSGFSRYAIYYTPQPGTALAAFGRSW
                     FGRANDGATLQAFSTSGLTGIATPSATPGRYTGLHAPFRAPFTLRDGVALDALRTRLI
                     TFTAHRKPVATGPLTLARAGRHLVLRPVDATPALDWLATQCVSAFEDFAVAPEDEDAT
                     DYRHLSAYQRLLLKSFGHPHVMSEYRFSIALAGPLEAAHLERVSQALWPVLEDICAEG
                     VTLDGLSLFGDAGGRAPMRQIGRFGLGG"
     gene            12064..12252
                     /locus_tag="AC16Y7_06685"
     CDS             12064..12252
                     /locus_tag="AC16Y7_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709103.1"
                     /translation="MPDYGEILAFLAANPPRDNVERELHASLLELLAKCLHSEQVAVG
                     DEDEDTLEPDEIRWMTIN"
     gene            12428..>13152
                     /locus_tag="AC16Y7_06690"
     CDS             12428..>13152
                     /locus_tag="AC16Y7_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018063044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709104.1"
                     /translation="MILSTEATFTMRDSVAVQQSDAAKSTAEDIIRAFSQSVANGDRS
                     DQPYAHWVLRGCLPEDTHQDVLNLPFPAPSLEGVSGKRELHNKTRKYFDAENKEAFPV
                     VAAVAEAFQDKRITDLIAKTFETNLDGTYLRIEYAQDTDGFWLEPHSDLGVKSFTLLL
                     YLSDDESHENLGTDIYDGDKKHVGRSPFAPNAAMIFVPADDTFHGFERRPIKGVRKSL
                     IINYVTDEWRAREQLAFPEAPINA"
BASE COUNT         2575 a         4153 c         3943 g         2481 t
ORIGIN      
        1 gcttacagct gcaaaagtgg acatccctaa tccggcttcc aatgtctgct aatgaccaaa
       61 agcgggcgta tgctggtcgc tggcagtaac tagttacctg ccggggaaac cctgcagaga
      121 gcgcgtgacg tcgctgcctt gcggtttcca tcgtacggac ttagacaccg cccgtcgccg
      181 tttacccttc cgggtcggca tggggaagcc ttctgcatcg ccgaacaggg cgcgtgcgag
      241 tttgtggtcg agcccgtaaa ggtccggaaa agtcgtgacc ttaccctgct cgtcggctac
      301 cgcggtgaaa tacgtcgagt agaccggaat ctcggagtca agcgtcactc ggttgttgat
      361 gctgttatcc cagagatcag tgactttgct ctccggccat ccattgctct cagcaaggag
      421 taccttcgcg aagcgatcag gcttttccat acgaacgcag tcatgaccga tcgcacgtac
      481 agcctgttga taatatttac gccgagcggg ccaagtgtcg tgcaggacga aggcatgtct
      541 gttgggcagc aggaacttta ccttgccgat attgctgtgt gggcccggcc tctgggtgaa
      601 ggtgtagttg agaatgttca cgcggcgcca gtcgatcttc gtcgggtcta cgcgcttgcc
      661 ttggtagctt accgagaacc ccttcttctt tagtatcgag tagtttttct tgcgcaatgc
      721 gggcgctagg ttctcccgca gcaccgtcgg cggcgcgatc cattcgggat tgaacacgac
      781 cgtcgtcatg tcggctgata gaacgggcgt cgcatagtca atcgtgccga cctgtgtctt
      841 gtccgcataa atggtctggc cgtctttcac gacgtagagc atgaatgcgg gcgtattgct
      901 ccatacgtag acgcggccta gatcttccgg catccagcgc cagcgctcca tattcatgat
      961 gatgcgcttg acgtcggttc tggaatcgcc ggcggcgtcg ccgtcccgtg ccacgagcaa
     1021 agtttggcgc aggcgcgcga actgggtgtg ttggggatga agcgaccgga ggtaggcgtc
     1081 tggcgtctcg ctccccgcga tgtcggcgag cacggtcttc ggatcgagca tcgacggcga
     1141 ttggaacacc ttgccaccga gggcagtggg gtcggaccgt gcaccgcggg caaagcgggc
     1201 atatcgcaga attgcgagat tgagcttgat ctcggcaatg gcctgttctt ctggccccgt
     1261 cggcaaagcg ctggccggcg gcagatcgaa cgcgtgcttg tcgagacccc aatcgtcggc
     1321 cttctcgatc tcgaacagag cctgttgtgc ccttgccgat aagcccatgt ccgttgtcca
     1381 caagggcacg ctagaccgat catcgtagaa ggcttcgagc gcggcgagat cgtccgcagc
     1441 tacccccttg cgcaagtcag aatcagcaag cttcgcgcca atggccgcca caacggcctg
     1501 gggcgcgggt gtcgaaggag ttggtgcctt cgacgtcgaa ggcgtggaac ggacggccgg
     1561 cggcgcgact gggtcctcag cgcacgccga gcgcatgacg gcaaaggtca ggacctgggc
     1621 cgcgcacaag aaaactggca caagacaaga accacgcatc cccttggttc cttaaggctg
     1681 ctgcgagatt accgcgctgc agcacacgcg gccataactt tgctccatcc cggattgaga
     1741 agtagcggct caatgtcggc ttattgagaa gaggcacccc cctcccccac ctaccccctt
     1801 cgtgattgtc gacttgtcac agctgtgtca cagcacggag ttcaagacgg gcggttcgat
     1861 gagctcagag aaagatcgta agctgcgtaa gcggctgatt tatcgatctt tcgggaggag
     1921 ccgttaggtg acgggctggt agagcactgt gttcacatcg caggggtcac tggttcgagt
     1981 ccagtaccgc ccaccattca cataatcgcc aacgcacctt cgatatccgc tcacaggacc
     2041 tcgggccgat cgccgagacg tggccccaca gctagctcgg gaatgctaca cttggcgcca
     2101 catgacccta ggtgccagcg atgaagatgc ttgccgtggt cctgacggct ctgattgcct
     2161 tcgtcctatt tcaatcgtac cggcacgact gccattggcg cgggcagaac cactgggccc
     2221 agtggagcgc ttgcatcctc gatgtcccgg gttagcccca acaggcaaat actcaaaacg
     2281 cggatactca gacgaaaaaa cccggccagg agaatctggc cgggtttttg acatttgccg
     2341 gagagataga ctaaagggct atctcgtccc ttagcccagc ttcaaaggct caaccacaac
     2401 ggctttctcg tgatggccga agcagccgcc gagaccgatc gtggcggaaa tgataaccgc
     2461 acatgcaatg atgacacgca ttggtagttc ccccaaaaat tccattcaga ttcgaaacct
     2521 atggctaggt tataacctgt tcgggcctgg cagactgtag catcagggcc acagacggga
     2581 atttcttcca ccttaggggc actttccagg catgcgcggc aagccgctca agccacggca
     2641 aaaccggcat aattgggagg aatccaagga aaaccgggcg aagtgacgcc ccgaggggct
     2701 agcgcgtcag cggcgtcttg gcgatgctcg tgatgttgag ccggctggcg agtagaccat
     2761 cggacgcatg cgggtccagc caaatgctcg gccgacccct ggcctccgtg aaatccttcg
     2821 ccaccgtcgc accgatcatc accaccagcg tgacggccat cagaccggcc atgagttgtt
     2881 ggttcatgtt catcgcccgc cgactctgcg gcgacggcga tgaatcttcg gttgatagac
     2941 ttggttaaag aaaggtgtac ggcgccgggg ctgaccggcg ccgctcaagt ccctccgcgc
     3001 ctagtccttc ttgcggagaa gcgcgtccaa gctccaaggc ccgccaccga cacaggcgat
     3061 gtacaggaag atgaagcagt agaggatggc cgcatcgcca ccattgagca gcggatagac
     3121 gctcttgggc gcgtgcgcga tccaataggc cgcggccatg aggcctgaga gcacgaatgc
     3181 gactggccgt gtgaagagcc ccacgatcaa cagagcgcca ccgacgactt ccagaacacc
     3241 ggcgatccaa gggagtgaca tcgcctccgg tgtgaagttg gcggccggga agttgaacaa
     3301 cttcgcggag ccatgggcga agaagatcag cccggaaacg atccgcaaga tactgaggac
     3361 acgtggaccc cacacagtct gaaggctgtt ggacatggca gcaatttccc cctttgtttg
     3421 gcctctgatg agcgcttgga cggcggagtg tacccgctag cgcccggccc gtcagcacac
     3481 gctgttgtca ggaagacttg gccggagacg gcagcgaaat ggcatgccgc cgaaccaggc
     3541 gccctttgag atggagagaa ccatgacggc aaacgcaatt gggcggacgg caaagatcta
     3601 ccaattcccc gagggcggcc gcgccggttt gagtagcggt cgccatgagg caactgacat
     3661 tgaggctgta gcgtcgaagc gcacagcctt ggtcgcctgc gacggtgcct cctatcacga
     3721 agacgctatt cgcgaagagc aacacgtgcg cgagaattag cggcgcgttt ctcaacgaca
     3781 gtctacgaag ggcctgtctc gtgcgcgagc aggccctttt tgtgttttgg aaaaaacgct
     3841 aggcgcgcgc cggccacgcg gtatcggacg actcgtcgcc accgggcgca aacatcggca
     3901 gcatcggttc atccgtcagc acaacttcat cgaaagtacc gaagccgttg aaacgattcg
     3961 ccaagacgcg gccataggcg cctacttggc cgatctccag atagtcgcct tccccgacgc
     4021 aagccggcag gacgaacggt cccggcaggt agtccgagct gtcgcaagtg ggcccgtaaa
     4081 gcgcgaacgc cgtgtcaggt tccgcactct ctttacccgg cgtgaccagc cgcgccggga
     4141 accggaagcc ggaatacgca gcgtcaaaca acgagccgaa cgcaccgtca tttatgtaaa
     4201 gcgtgtcgcc gcggcgcgcg tcgacccgga cgatcaccga ttccgcctcg gccacaagtg
     4261 cgcggcccgg ctcgcacaaa agttcgcaac tgtgcttcac ggcaagttcg tccgccgcac
     4321 gaacgatctc ttccatgaag ctttcgagcg cgggaggatc ggcgtgcggg tagcgtgcgg
     4381 gaaagccgcc accgatatcg acagcgtcga caagcacgcc cgaggacacg atcaactgac
     4441 ccacctgccg caacacctcg ccgaaggctg ccggcaccac cgcctgcgaa ccgacatgga
     4501 aggtaatgcc gagacgcatc gcgcattggt gcgccttgag cagcagcgcc ggtgcttcct
     4561 cttgagaggc accgtacttc ccctccagcg gtatgaggct gtgcgtgttg gggcaggcaa
     4621 tgcgcaagta gagggtgaga tcgtcagccc cgtttgtttc gtccaacacc ttgtcgagct
     4681 cctcatcgga gtcgaaggca aagcgccgga cgccaaattc gcgataggcg cgcgaaatgg
     4741 caccgcgcga cttgatcgga ttcataaagt aaagctcggc gtcctcgacc cctgccacac
     4801 gttcgatctc gaccagggac gccacatcga aggcgcggat gcctgcgtcg gccagcgcgt
     4861 cgatgatcgg cggcgcgtcg ttggccttgg cggcgtagag cacgcgcccc ggaaagtttg
     4921 cgaggaacca ggccgccgca cgcgcaaccg tatgcggtcg aagcgcgaga acgggccgct
     4981 cagggcgatg atgacttaga agcttcgctg cggaaggata aacgtccatc ccatgcgtct
     5041 cccacacgag tttcggcctt cttgtaaagg gtttttccag gaccgcccag ggtcgcctgc
     5101 gttgctagtt cgacttcgcg tcggcaacgc ccgtacccga cagtgccggt gtcgcggcgc
     5161 tggaatagac cggctcctgc aacaagccga atgtcttgcg cgctgacaga cgcgccatgg
     5221 cggccacgaa cgcatagagt tcggcgctgt ccacgacacc atcgccattg ccgaccggat
     5281 agaggtcgcc ccggcccgca aggccttcga tgacgtagcg ggtgaagagc cccacgtcgt
     5341 accgcgggtc gatcagcgcg cgctgtccac ggtccgtgcc ggagagcacc gtgacgttgg
     5401 agagcggcgc ggacggcaac gccgagcttg tggtctcggg cagattgggc ggcagcacat
     5461 aggccccgtg atcgcgtcca taggcgctct ccagcaggac aagcacctta tccgccttca
     5521 actcggcaag attgtcgtag agcgtcgaca gcggatagcc gccccgctcc tcgcgatagg
     5581 gctccgcgtc caccggcagc agatatgtct cgctgtggct cgaattggtc gcgccgtgac
     5641 cggaataata gacaacgacc ttcgcgttgg gctgcgcgcg cactaggcgc gcaagctcgc
     5701 catccgaccc cgacaccggt ccgaagacgc gctcgaaatc ggccaggcgc gcgtttcgca
     5761 gatcgatgat gcgatcctgc cggaaaccca tgtactcggt cagaaagaaa tacatggcgc
     5821 cggcatcgtt tcgcgcggtc tccgccggcg gcaggttgtc gtacttgccg ttgccgatga
     5881 tgaccgcgat cgcgccaggg tcgtccgcgg cggggggcag cgctctatag agctcgcgaa
     5941 tgcggctgaa cgacttcacc acattgtcgt tggcgcttcc cggtcgcggc agctggcgcg
     6001 gcggcacggt acaaagctcg agattgccct cgtcttcgat gcgccggatg tcttccaagg
     6061 agaggcgccc ttcgatctgg aggcaagacc ggcaccaccc caccccatag cgcgcttgaa
     6121 gatcgtcgag ctgcgactgg ctcagcccct tgggcgaagg caagcaaccc cggcggcacg
     6181 ccggcgccct gacaccctcg ccatgcgcct tgcgcaagac ggcgatgagg tcctccggca
     6241 gacagtcaat ctcaagccgg gtcggcggcg ggggcagttc ttcggcaaca accggctccg
     6301 cattgggggc aagcgccgcg gtcaccgtat cggtatccgc gttctcgaat tcttctggtg
     6361 aattcggtgc gggcggctcc ggggccttaa cctcaaccgg ctcgggcctc ggctcaacgt
     6421 ccttgcaaag ttttttgatc ttcttctgga ccgcctcgga caggaaatcc ccgtgcttct
     6481 tcaacccatg gtcgttcttg aagtgccaat gcgcggtcca ggtcgcctca ttggccttcc
     6541 ccgtgatggc tcctttgtag tagccgagct tggcgtagca gcgctgtaca cgcgcggtcg
     6601 ctttggcttt gggatcgtgg agctccgccg gggctggcgt cggctccgac tccggggtca
     6661 ccaccgccac aacggacata tccgtgggcc gggcctcggc acccgccacg cccacaaccg
     6721 ccagggcaag cccgataatc aggccgcgcc cgcgccctcg tctccccgcc atatgaacct
     6781 ctacgcaaca ctcggcgatc gcgaaatacc gcacgcacca attcaaaaca ctcaaccagg
     6841 tccgaagcaa gagcggggcc aacccagccc cgccgcaaat ctgcatcccg ggccaaacta
     6901 ggaggcgatg gcggcaagag attggcggtg cgcccgtccc cgtcgatgaa acgtcagcgc
     6961 accaccagct tgatcgccgt ccgcgccagg tcctcggagg catcatcatc ctttggagtg
     7021 gccggcgcct catgttgcgt tttggctgga cgggccggct ggccttgggc ggccactttg
     7081 ggcgtaccct ccaccacgat ccgccgggtg aggaactccc gataattgcc gaccggcgtg
     7141 aactgccgcg tcttgaagtc gtgctcgatc ccgtcggctt ccactcctgt cgcgttgcag
     7201 atggcgatga gcgtctcggt gccaggatcc ttgagccggt actggaacgg cgcgtcgtcg
     7261 ccagggaact gcgcctccgt gttcgccggc aagaaattgt cctgctggaa ctgattgggg
     7321 aagatcacgg tgccgtcccc gccggcctcg acattgatca aggtgagatg acagcctttg
     7381 ctcgaggtga cgctgaacac agcaacgtcg ttcacgcgat acgtgctctt gtcggaggtg
     7441 agcgcgaggt cgaagtcgtc aaagaacacc tcgttctcat cctgaatcag ctcgacggcg
     7501 gtcggcgtca acgccgcgag cctgatgggc gcgttaccgg aaaccttggc gacgagatcg
     7561 ggaaactccg cttcgagaat ctgacgcggc aggacgccct gctccaaccg ccgcttaatg
     7621 cgtaccgcct caccgccggc atccgccagc ccttgcgcca gcgcttctgc ttcgatcccg
     7681 tactccgcag cggcgatgcg aagatcgacc gacttctcgt aggccttcga taaaaagttg
     7741 atgctctcca cgccttgcgt ctgagagtcg agctccgggt cgagcccggc tgcgagcatc
     7801 gccttgcgga atttctcggc gtcctgcgtc agcagcgcct tcatctcatc ttgcggcgcg
     7861 tagagcgcat cgaccgtctt gcgaacgccc tccggaaagc tccgatcgcg aagcacatgc
     7921 gcgcgaatgt catccgtcgc ttggcgaatg ccttggttat gacacccgaa gcaggagatc
     7981 gcgttggtca ccgcgcggtc gcgttgactg tcgtcgagca cgatttcggt aggccctttg
     8041 ccgagacgct cgccctcggc cgtgttcagg taataagcct gaaacccgtt cggcaggctg
     8101 aagatcgtct caccgccgtc gtggcggaag gcaccgtcgc cgaagggacc gagcgggtgc
     8161 acgaacaggc tttgtgccgg cgcatcgccc ttgaagtcgt acgacgtcca gaaatagccg
     8221 gtcgggatcg cgtgccgctc aatcaaccgg ttctgtttgg agacaccgga cttctgaaac
     8281 ccggcgcgcc gggcaagata cgcctcgatg ttcgccttca catcgacgcc ttcttgccgt
     8341 tcgagttcgg cgaaagtcgt cggcagcttc aggagatcgt agtagagcgg cggacgtgac
     8401 gcgatgaagg cgagccaatc gccgcgcagc cacggaagct cggcgccggt cgcggcaacc
     8461 acatcacgat aggccggcac gctcggcgcc atgccataag gatagagccc ggcgagacgc
     8521 gcccagtcct cggtcgtcca attgaggtcg gcgaggttga aggcaatgat tgttgcgtgc
     8581 ggatcgatgg tccgcattgt caacggcgtg ggattgcggc tgagactatt cagtaatttc
     8641 accacgccct gccggtagcg cgtcatgtcc tcgccggatg cacaggcatt atagaagtgc
     8701 gtcagtgtca cgtagcgcat cccggggcgt cggcgcatct cttgtgcctt gatgtctttg
     8761 gcgatggcac gcacgaccac cgcttcgcca atcggtttcc ggttgccgca ggccgcgcca
     8821 accgtatcgc caagacttct gatccagtcg tagatcgcct tgacgtcagc ctccgtagga
     8881 tcggccttac agtcgaactc ctggtagcag tcgtagggca tgtccttttt ggcgatctga
     8941 atgaaaagac tggagccgtc gggattgccc ggcagtatca agtccggatc gcgggcgatc
     9001 tcggtgagct tcagcacatt gccgaaattc tttgcaggct tctgccgctt caggctggga
     9061 ccggcctggt gacaccgggc acaatgtttg tcgagcgcat caaaggcggc acgcgccacg
     9121 ccgtcctgag gcttggacat gtttgcagac gtttcggcat gcgcatggcc gaagccgatc
     9181 gccatgcagg acagcatagc gagggctacc aatctatggc gcacgcacca catcgtcagg
     9241 acctcccttg accgagacca gttccttttt actgctttag gccgcggcaa ctgaacctcg
     9301 catgaacgat ccctagcttt ggtcatcctc atcgtcggcg ttcgtcttgg atttctgcac
     9361 ggcgttggga cgggacggca acgtcttgag gaaagcggca atggcctcgc ggtcctttgc
     9421 cgagagcttc tccatattct cctgcaccgg cgccatggtg tcgccgatca cgtcgaaatc
     9481 cggcgtgctc cccgtctcca acaaatagac gatgtcctcc tcgctccaat cgccgatccc
     9541 gtctgcgctc ggtgtgatgt tgggaatcgt ccccttcccg tccggatttt tggcgccagc
     9601 gaacgcttcg tccgcgatga tgccgccgaa cgcgttccgg ggcgagtggc attcgccgca
     9661 gtggccaggc ccgcgcacca gataggcgcc gcgattaacc tcgtcactga ccgcaggatc
     9721 cggtgcgaat gttttgccgt cgacatagag ccgttggaac aggcccagcc cgcggcgaat
     9781 gttgtaggga aagcgcaacg tatgcgccgg cgtctcgttg cgcaccgccg gcagcgtatc
     9841 gagatacgct ttgaggtcga tgaggtccgc ataggtcatg cgctgatacg aggtataggg
     9901 gaacgacgga taaagatgca cgccgcccgg cgccacaccc cgcttcatgg cagagacgaa
     9961 atcgagcgtc gtccagttgc cgatgcccgt ttccgcatcc ggcgaaatgt tcggcacgta
    10021 gaacgtgccg acatcggtgt tcagacaacg accgcccacg agcaccgtct cgtcttcgat
    10081 atcgagatcg tcgcatttgc cgataggctt ggcgtggcac tccgcgcaac cgccggcgat
    10141 gaacatgacc ttaccgttgt cgatgacgga cgtgtgattg ggcaaggccg acgccggcac
    10201 ggtcacaggc atcgtgagac cgtagaaccc gatcgcacca atcgcgatca acccaaccag
    10261 aaaaagaagc gcccggcgca aaacgttggc ctccgtccgt ggagcacgtc acccgtgcgg
    10321 gccgtatcct gccatgcgcg tgcagcccct gctacaccgc gcaagtctca cggaaaatct
    10381 cacggcatgg gcatgacccg cccgacctcc aagacgtccc agccgcgttg caccgcggga
    10441 cggtttgcga tcgcgacata ccagcgcttc acattgggat gctcatcgaa gtccactgtc
    10501 tgccactcaa agcgcgaaat ccacggccag atcgctatgt cggcaatcga gtagtcgccc
    10561 gcgacatact ccacttcgcc taagcgccgt tcgagcacag accataggcg cttcgattcc
    10621 ttggagtagc gctcttcggc ataggtgctc tttccggggc taaccttcac gaagtgttgc
    10681 acttgaccga ggaacggacc gacgccgccc ttctgccaca taagccactc cagcacgcgc
    10741 cacttggtgg ggaagtcatg gctccacagt ttgccggtct tctccgcgag atacatcagg
    10801 atcgcgcccg actccatcaa ggtcagctca ttgtcgtgat cgacgatggc cgggatcttg
    10861 ttgttgggag agattttggt gaactcgggc tcgaactgcg cgccaccatt ctcgatatcg
    10921 accggatgga cgcggtacgg aagcccaacc tcttccagca tgatcgacac cttctgtccg
    10981 ttcggcgtca tccaggtata gagatcgatc attgcgtcct cgcccgtctc aacatgttgc
    11041 gtccgcttcc gcagtgcacg cgcacttgtt cgcagtcgcg aaaagctcga gtcaccaagt
    11101 cttcatgtcc ccgccgcgct atcgcccgaa accggtcgcc tcgacgccac aggccgttgt
    11161 tagccgtgaa ggcacaaacg ccgcgatggg aaacgcgcac ggaggggaga catagatgtt
    11221 caattgtgcc cacgttgaca accatatccg tgaagacgcc gcatcgaacc gctcgggatt
    11281 ttcccgctac gcgatctact acacgcccca gcctggaact gcactcgcgg cattcggacg
    11341 ctcttggttc ggccgcgcca atgacggcgc caccttgcag gcgttctcca cctctggatt
    11401 gaccggcatc gccacgccct ctgccacgcc gggccgctac acgggtctgc acgcaccctt
    11461 tcgcgcaccc ttcacgctgc gcgacggcgt tgcgttggac gcactgcgga cgcggctcat
    11521 caccttcacc gcgcaccgca agccggtcgc gacggggcct ttgactctcg cccgcgcggg
    11581 ccggcacctc gtgctgcgtc ccgtcgacgc cacgccggca ctggattggc tcgccaccca
    11641 atgcgtcagc gccttcgagg acttcgccgt cgcgcccgaa gacgaggacg ccacggacta
    11701 tcgccatctc agcgcctacc agcgcctcct gttgaagagc ttcggccatc ctcacgtcat
    11761 gagcgagtac cgcttctcga tcgcgctcgc cggcccgctc gaagccgccc atctagaacg
    11821 cgtctcccag gcattgtggc cggtgctgga ggacatctgt gccgagggcg tcacgttgga
    11881 cggcctgtcg ctgttcggcg atgcgggagg ccgggcgccc atgcgccaga tcggccgctt
    11941 cggcctcggc ggatagggcc cgcaaggggc gccgacggag cgccggccca ccccgaacgc
    12001 gagcctaagt tttgacacac ggctccgcca ccctcgtttt gctgggaaaa aacgggggtt
    12061 ccaatgcccg actatgggga aatcctggcg ttcctcgccg ccaatccgcc acgcgataat
    12121 gtcgagcgcg agctgcacgc ctcgttgctt gagcttctcg ccaagtgcct gcactcggag
    12181 caggtcgcgg tcggcgatga ggacgaggat acgctggagc ccgacgaaat ccgttggatg
    12241 accatcaact gacccagcgg cgccagaagt tgaccaacgt caaggtccgc ggctcggact
    12301 gatttaggca aagtcgtcgc ggaattgacc ggacgcatcc cagccttggc atttgcgccc
    12361 gaggggaccg gggtgctatt tgagcggttt ggatgggtct gcctgggcgc tggaaagacc
    12421 agcaaatttg atcctttcga cggaggcgac cttcaccatg agggacagcg tggcagtaca
    12481 acaatcagac gcggcgaaaa gcacggcgga agacatcatc cgcgcatttt cgcagagcgt
    12541 cgcgaacggc gatcgttccg atcagcctta tgcacattgg gtcttgagag ggtgcctacc
    12601 tgaggacacc catcaggacg tgctcaatct ccccttccca gcaccgtctc tcgaaggcgt
    12661 gtcgggcaag cgagagctgc acaacaagac gcgcaaatat ttcgatgccg agaacaaaga
    12721 agccttcccc gtcgtcgcgg cggtggccga ggcttttcaa gacaagcgca tcaccgacct
    12781 gatcgccaag acgttcgaaa ccaatctcga cggcacttat ttgcggattg aatatgcgca
    12841 ggacaccgac ggtttctggc tggagcctca ctccgatctc ggcgtgaagt ccttcacact
    12901 gcttctctac ctctccgacg acgagagcca tgagaatctc ggcaccgaca tctacgatgg
    12961 cgacaagaag catgtcggcc gctcgccgtt tgcgcccaac gcagcaatga tcttcgtgcc
    13021 ggcggacgat acgtttcacg gattcgagag gcgtccgatc aagggcgtgc gcaaatcgct
    13081 gatcatcaac tacgtcaccg acgaatggcg cgcgcgcgag caactcgcct tcccggaggc
    13141 gccgatcaac gc
//
LOCUS       DBNHVU010000164         6269 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1992193, whole genome shotgun sequence.
ACCESSION   DBNHVU010000164 DBNHVU010000000
VERSION     DBNHVU010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1992193"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1471
                     /locus_tag="AC16Y7_06695"
     CDS             <1..1471
                     /locus_tag="AC16Y7_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR5709105.1"
                     /translation="ELIGLFSQGQFPEALQYKPDSKPYKSAWKETVDAAEEYNDPGRF
                     TAFIGYEWTSLAKGNNLHRVVIYRDGAEKALRMVPYTTVAPFGSTDPLELYKWLEKFE
                     NDTGGDVLAISHNGNLSNGIMFPIDEQYTGRPLDKLYVEQRDKWEPLYEVTQIKGDGE
                     THPFLSPEDEFADYETWDIGNLDASEAKTDEMLAGEYARSALKRGLQLEKKHGTNPYK
                     FGMIGSTDSHTSLATAQEDNFFGKHSGAEPNPERMAHPFMKTDAGTIHGWQQVSSGLA
                     AVWAPENTREAIFDAMARKETYGTTGSRMGVRFFGGWNYTDKDIASRTPAFAGYEKGV
                     PMGANLPAKPDDAKAPNFMVYALKDPIGGNLDRIQIIKGWMDADGKTHEKVYDVVWSD
                     DRKPGADGKLPPVGNTVDKKSATFSNTIGESELGTVWTDPDFDPGQKAFYYARVLEIP
                     TPRWSTYDAFRFGVDIPEGAPVSQQERAYTSPIWYTPKG"
     gene            1626..3530
                     /locus_tag="AC16Y7_06700"
     CDS             1626..3530
                     /locus_tag="AC16Y7_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR5709106.1"
                     /translation="MKFPSVASTFSAVLLATALVSPVAADQLPTKEGTADIAKPQFSP
                     YAGRNFPTKVYWGDTHLHTDVSVDAGTMNRIGQEDAYRFARGEEVTTTHGLQAKLSRP
                     LDFLVISDHAEMYGLMPQLLGGNQEILSTETGKRWYDALSAGDKDKTFDTAMEIVRSL
                     SGDEPPIKSDKAVRDAWQNYTALADKYNDPGKFTALIGYEWTAIGGNNLHRNVIFRGD
                     SSVANRTVPFSQFDSKNPEDVWKHLAAFEKETGDEVLAIPHNGNLSNGRMFRVETFDG
                     QPLTKELAAMRAQYEPLVEVTQIKGDGEAHPMLSPNDEFADYETWDKSNLNGTEAKTD
                     DMFQWEYAREALKTGLKLDKTLGVNPYKFGMIGSTDAHTSLAAVEEENFFGKHSGVEP
                     EPHRWEHVVIEAPDPKFTIKGWQQAAGGYAGVWATENTREAIFDAMKRKETYATTGPR
                     MTVRFFGGWDFDEDDTNTRLPATAGYAKGVPMGGDLSAAPDGKAPTFLVAAAKDPYSG
                     NLDRIQIVKGWMNADGETEEKVYDVVWSDDRKPGADGKLPPVGNTVDVGNATWSNSIG
                     DPEMIAAWEDPDFDPDQRAFYYARVIEIPTPRWTAYEAKRFNVEMPDEVPMTTQERAY
                     TSPIWYSPKG"
     gene            3554..5398
                     /locus_tag="AC16Y7_06705"
     CDS             3554..5398
                     /locus_tag="AC16Y7_06705"
                     /inference="COORDINATES: protein motif:HMM:NF023649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HIR5709107.1"
                     /translation="MKAVAQVAGFAILLTAFVVADDARGAAEQSLADREAAVPENPLK
                     DAYFGEQHVHTSYSLDAYIGGEALTPDDAYRFAKGNAVTVYGKPHSIGRPLDFVAITD
                     HAEFLGEMYAAQVEGSPGYDNPTLVELRGLDNFDDQEAWFLKYVVSSNRSATPQHTDF
                     YPGDDALHNGWKVMVDAAERHYVPGTFTTIPAFEWSAAPKGGNMHRNVFFHDTKVPRL
                     PFSAVDSNNEEELWDWLAEQERAGSTLLAIPHNSNASKGFMFEPVDNKGEPIDADYAR
                     TRAKWEPLIEMMQIKANSEVNRKLWPADEFADFENGDSIQNYSDRTFKKENFVRWAAI
                     KGLHYDKMLGVNPYKFGFVGGTDSHNGTPGDVAEANYVGSHGGADGPVKRRRDEDIAG
                     WIMGRDSNPGALTGVWAPKNTRGAIFDAMKARETFATSGPRIKVRFFGAPGLATSSDP
                     VALVKEGYKQGVPMGGTLEDLGEAPTFTVHAMKDPVGANLDRIQIIKGWVDDEGEPQE
                     KIVDVVWSGSRKQDADGKLPPVGNTVDVKTARYENSIGSAELIGSWTDTEFDPKQHAL
                     YYTRVLEIPTPRWSTYDAVRNDLPLLSDVPSSVQERAWTSPIWYSPKG"
     gene            5421..6008
                     /locus_tag="AC16Y7_06710"
     CDS             5421..6008
                     /locus_tag="AC16Y7_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709108.1"
                     /translation="MHCTRPLAAAALLMAAIVFAGQAALACGYHDPRSMARGLMNLVF
                     PKSLYVSTAVWRAQRSGVLPPRQRKAAKDLFAYQRDVSNLQKLGARLVAPSREEGSFA
                     LVLLDSMLWTRFVSGPDGYTVATHVKGPEKGDAVLVTESAVIKALLDGSIDMPAVEAK
                     GLFRLYGPVERQAATRNALRGLSTKSPAASLEKAG"
     gene            6015..>6269
                     /locus_tag="AC16Y7_06715"
     CDS             6015..>6269
                     /locus_tag="AC16Y7_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR5709109.1"
                     /translation="MTVFTRLLREPLVHFVLIGAAVFGLYALLSDGGEERVRDRIVVS
                     EGRVKQLAQIFAKTWQRPPTPQELRGLVDAFVKEEVYYREA"
BASE COUNT         1348 a         1838 c         1927 g         1156 t
ORIGIN      
        1 ggagctgatt gggctcttct cgcaaggcca gttccccgaa gccctacagt ataagcccga
       61 ttccaagccc tataagtcgg cttggaagga aacggtcgat gcagccgagg agtacaatga
      121 cccgggccgt ttcaccgcgt ttatcggcta tgagtggact tcgctggcca agggcaacaa
      181 tctgcatcgc gtggtcatct accgtgatgg cgctgagaag gcgttgcgca tggtgcccta
      241 cacgacggtc gcgccttttg gcagcaccga tcctctagag ctctacaaat ggcttgagaa
      301 gtttgagaac gacacgggcg gtgacgtctt ggccatctcc cataacggca atctgtcgaa
      361 cgggatcatg ttcccaatcg atgagcagta tacgggccgc ccgctcgaca agctctatgt
      421 ggagcagcgc gataaatggg agccgctcta cgaggtaacg caaatcaagg gcgatggcga
      481 gacccatccc ttcctgtcgc cggaggatga gttcgccgat tacgagacct gggacatcgg
      541 caatctcgat gcgtccgagg ccaagaccga cgagatgctc gccggtgagt atgcgcgctc
      601 cgcgctgaag cgtgggcttc aacttgagaa gaagcacggc accaatccct acaagttcgg
      661 catgatcggc tcgaccgaca gccatacttc cttggcgacg gcgcaagagg acaacttctt
      721 cggtaagcac tccggcgcag aaccgaaccc ggagcgcatg gcgcatccgt tcatgaagac
      781 cgatgccggc acgatccatg gctggcagca ggtgagctct ggccttgcgg ccgtctgggc
      841 gccggagaat acgcgtgagg cgatcttcga cgccatggcg cgcaaggaga cctatggcac
      901 gacgggcagc cgcatgggcg tgcgcttctt cggcggctgg aactacaccg acaaggacat
      961 cgccagccgt actccagcct tcgctggtta cgaaaagggc gtgcccatgg gtgccaatct
     1021 gccggcgaag cctgacgacg ccaaggcgcc caacttcatg gtctacgcgc tgaaggaccc
     1081 gatcggcggc aaccttgatc gtatccagat cataaagggc tggatggatg cggatgggaa
     1141 gacccacgag aaagtctacg acgtggtgtg gagcgacgac cgcaagcccg gcgcggacgg
     1201 caagctgccg ccggtgggca acacagtcga caaaaagtcc gccacgttct ccaacacgat
     1261 tggcgaatcg gagctcggca cggtgtggac ggacccggat ttcgatccgg gccagaaagc
     1321 cttctactac gcgcgcgttc tggaaatccc gacaccgcgc tggtcgacct atgacgcctt
     1381 ccgcttcggg gtcgacatcc ccgaaggggc acctgtctcg caacaagagc gggcctacac
     1441 ctcgcccatt tggtacacgc cgaaagggta agcgccgtca cgcgagcaac cttaagcttg
     1501 gactggggac ctcacgggga accccagttc cgtggcgtga gttgaaaaac tgacccatag
     1561 attgtggtat gcaacctgag gcagctgcgc ctatggcaat gcctcggctg ttcaaggaga
     1621 aaacgatgaa gttcccctcg gttgcctcga cgttttccgc cgtgcttctc gccacggcac
     1681 tcgtctcacc cgtagcggcc gatcagcttc cgaccaagga aggcacggcg gatatcgcca
     1741 agccccagtt ctcgccttat gcgggacgga acttccccac caaagtttat tggggcgaca
     1801 cgcacctgca cacggacgtg tccgtcgatg ccggtaccat gaaccggatc ggtcaagagg
     1861 acgcctatcg cttcgcgcgc ggtgaagagg tcaccaccac acacgggctt caagccaagc
     1921 tctcccggcc gctcgacttt ctagtgattt ccgatcacgc cgagatgtat ggcttgatgc
     1981 cgcaattgtt gggcggcaac caagagattc tatcgactga gaccggaaag cgctggtacg
     2041 acgctttgtc ggccggcgac aaggataaga ccttcgacac ggccatggag attgtgcgct
     2101 cgctgtcggg cgacgagccg cccatcaaaa gcgacaaggc cgtgcgcgat gcttggcaaa
     2161 actacacggc gctcgccgac aaatataatg accctggcaa gttcaccgcg ctgatcggct
     2221 atgaatggac ggccattggc ggcaacaatc tgcatcgcaa tgtaatcttc cgaggcgatt
     2281 caagcgtcgc caacaggacc gttcccttct cgcaattcga cagcaagaac ccggaagatg
     2341 tttggaagca tctcgctgcg ttcgagaagg aaacaggcga tgaggttctc gcgattccac
     2401 acaatggcaa tctgagcaat gggcgcatgt tcagggtcga gacgttcgac gggcaaccgc
     2461 tgaccaaaga gttggccgct atgcgggccc aatatgaacc gctcgtcgaa gtcacccaga
     2521 tcaagggcga cggcgaagca catcccatgc tgtcaccaaa tgacgagttc gccgactacg
     2581 agacttggga caagtcgaat ctcaacggca ccgaagccaa gaccgatgac atgttccaat
     2641 gggagtatgc ccgcgaggcg ctgaagaccg gtctgaagct cgacaagacg cttggcgtga
     2701 acccctacaa gtttggcatg atcggttcga ccgacgcaca cacttcgctg gctgcggtcg
     2761 aagaggagaa cttcttcggc aagcactcgg gtgtcgagcc ggaaccgcac cgttgggagc
     2821 atgtcgtcat cgaggcgccg gatccgaaat tcaccattaa aggctggcag caggccgcag
     2881 gcggttatgc cggcgtctgg gcaaccgaga acacgcgtga agcgatcttc gacgctatga
     2941 agcggaaaga aacctacgcg acgacggggc cgcgcatgac ggttcgcttc ttcggcggct
     3001 gggacttcga cgaggatgac accaacacgc gtcttcccgc gacggccggc tacgccaagg
     3061 gcgtgcccat gggtggagac ctcagcgccg cgcctgacgg caaggcgccg accttcttgg
     3121 tcgcggcggc gaaagatccc tatagcggca atctcgaccg catccagatc gtcaagggct
     3181 ggatgaacgc cgacggggag accgaagaga aggtctatga cgttgtgtgg agcgacgacc
     3241 gcaagccggg agccgacggc aagctgccgc cggttggcaa taccgtggat gtcggaaacg
     3301 ccacgtggag caactcgatc ggcgatccgg agatgatcgc ggcatgggag gacccggact
     3361 tcgatccgga ccagcgcgcg ttctactacg cgcgggtgat cgagatccca acgccccgct
     3421 ggactgccta cgaggccaag cgcttcaatg ttgagatgcc tgacgaggtg ccaatgacca
     3481 cccaggagcg tgcgtacacc tcgcccattt ggtattcgcc gaaggggtaa atggcattga
     3541 agaatgaacc caaatgaaag ctgtcgcgca ggtcgccggt ttcgctattt tgctgacggc
     3601 gtttgttgtt gctgatgatg cgaggggcgc cgccgaacag tcgctggcgg accgcgaggc
     3661 agctgttcca gagaaccccc taaaggatgc ctattttggc gagcagcatg tccatacgtc
     3721 gtattcgctc gatgcctata tcggcggcga ggcgctgacg cccgacgatg cctatcgctt
     3781 tgcaaaaggg aacgcggtaa cggtttacgg caagccgcac agcattggcc gtccactcga
     3841 cttcgtggca attaccgatc acgcggaatt tctcggtgag atgtatgccg cgcaagtgga
     3901 gggatctccc ggctatgaca acccgacgct tgtcgagctg cgcggtctgg acaacttcga
     3961 tgatcaggaa gcctggttct tgaaatacgt cgtatcaagc aaccgttcag caacgccgca
     4021 gcacacggac ttctatccgg gagacgacgc cttacataac ggctggaagg tcatggtcga
     4081 tgcggcagaa cggcactacg tgccgggcac gttcacaacc atccccgcgt ttgaatggtc
     4141 ggccgcgccc aagggcggta acatgcaccg caatgtgttc ttccacgaca ccaaggtgcc
     4201 tcggctaccg ttcagtgctg tcgactcgaa taatgaggaa gagttgtggg actggctggc
     4261 ggagcaggag agggccggct ccacgctgct cgccattccc cataactcga atgcgtccaa
     4321 gggcttcatg ttcgagcctg tcgacaacaa gggcgagccc attgatgccg actacgcgcg
     4381 cacccgcgcc aaatgggagc cgctgatcga gatgatgcag atcaaggcaa actccgaggt
     4441 gaatcggaag ctttggccag ccgacgagtt tgcagatttc gagaacggcg acagcatcca
     4501 gaactacagc gatcgcacat tcaaaaagga aaattttgta cgctgggcgg ctatcaaggg
     4561 gcttcactac gacaagatgc tgggtgttaa tccctacaag ttcggtttcg tgggcggaac
     4621 ggatagccac aacggcacgc caggtgatgt cgccgaggcg aactatgtcg gaagccacgg
     4681 tggcgccgac ggtccggtga aacggcggcg cgatgaagat attgcgggct ggatcatggg
     4741 ccgagattcg aacccgggcg ccctgactgg tgtttgggcg ccgaagaata cgcgcggcgc
     4801 gatttttgac gcgatgaagg cgcgcgagac gttcgcgacg tccggtcccc gcatcaaggt
     4861 gcgtttcttc ggtgctcccg gactcgccac ttcgagcgat cccgtagcgc tggtcaagga
     4921 gggctacaaa caaggtgtcc ccatgggcgg tacgctcgag gatttgggtg aggctcccac
     4981 cttcaccgtg cacgccatga aggaccctgt tggagccaac ctcgaccgca tccaaatcat
     5041 caaaggctgg gtggacgacg aaggtgagcc acaggaaaag atcgtggacg tcgtctggtc
     5101 cggttcgcgc aaacaagacg ccgacggcaa gctgccaccc gtgggcaaca ctgtcgatgt
     5161 caagacggcg cgctacgaga attcgatcgg cagtgccgaa ctgatcggaa gctggacgga
     5221 taccgagttc gatcccaagc agcacgcgct atactacacg cgtgtcctgg agataccgac
     5281 gccacgttgg tccacttacg atgccgtgcg caacgatttg ccgttgcttt cggacgtccc
     5341 gtcgtcagtc caggagagag cctggacgtc gccgatctgg tattcgccga aggggtgagc
     5401 gagttatggg acggcagatc atgcattgca ccagacctct ggcggccgcc gctttgttga
     5461 tggccgctat tgtctttgcc ggccaggcgg cgcttgcgtg cggctatcat gacccgcgct
     5521 cgatggcgcg cgggctcatg aacctggtgt ttccgaagtc tctttatgtc agtacggccg
     5581 tttggcgcgc ccagcgtagc ggcgtcctgc cgccacggca gcgcaaagct gcgaaggatt
     5641 tattcgccta ccagcgcgac gtttccaact tgcagaaact gggcgctcga cttgtcgcac
     5701 caagtcgcga agagggatcc tttgcgttgg tgttactgga ctccatgctc tggactcgct
     5761 tcgtttccgg acccgacggc tataccgtcg ccacgcatgt caaagggccc gaaaaaggcg
     5821 atgcggtcct cgtgacggaa tccgccgtga taaaggcgct gctcgacggc tcgatcgata
     5881 tgcccgccgt ggaagccaaa gggctgttcc gcctctatgg tcccgttgaa cgccaagccg
     5941 cgacccgcaa cgccttgcgc ggtctttcga cgaagagccc ggcggcgtcg cttgagaagg
     6001 ctggctagcc cggcatgacc gtgttcaccc gactgctccg cgagccgctg gttcatttcg
     6061 tcctgatcgg ggcggccgtg ttcggcctct atgccttgtt gagcgatggc ggcgaggagc
     6121 gcgttcgcga ccgtattgtg gtatccgaag gccgcgtaaa gcagctcgcg caaatttttg
     6181 ccaagacttg gcagcgcccg ccgacgccac aggaattgcg cgggctggtg gacgctttcg
     6241 tcaaggaaga ggtttactac cgcgaggct
//
LOCUS       DBNHVU010000165        21049 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1173809, whole genome shotgun sequence.
ACCESSION   DBNHVU010000165 DBNHVU010000000
VERSION     DBNHVU010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 21049)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21049)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21049
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1173809"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /gene="thrS"
                     /locus_tag="AC16Y7_06720"
     CDS             <1..1294
                     /gene="thrS"
                     /locus_tag="AC16Y7_06720"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR5709110.1"
                     /translation="RIYGTAWASEDDLKAYLHQLEEAEKRDHRRLGREMDLFHFQEEA
                     PGAVFWHPKGWALFQALIGYMRRCQQAWGYFEVNSPDMMERTLWEQSGHWEKFAENMY
                     IAETPDDRVFCCKPMNCPGHVQIFKNGLKSYRDLPLRIAEFGKVHRYEPSGALHGLLR
                     VRHFTQDDAHIFCTEDQITEECVKLNAQMLSIYRDFGFEDVRIKFSDRPEKRVGDDKV
                     WDAAEAALMRAMEAAGLDYELNPGEGAFYGPKLEYVLRDAIGRDWQCGTVQVDLSMPE
                     RLGAFYIGADGEKAVPVMIHRAMFGSLERFTGILIEHHAGHLPLWLAPTQAVVATIVS
                     DADAYAEQVLARLRGAGLRAETDLRNEKINYKVREHSVAKVPVMLVVGKREAEEGTVS
                     VRRLGSQKQEVLSLDDVLKMLEAEAVPPDMREGKSAAA"
     gene            complement(1298..3112)
                     /locus_tag="AC16Y7_06725"
     CDS             complement(1298..3112)
                     /locus_tag="AC16Y7_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR5709111.1"
                     /translation="MRGIRIAALKLLALPACMLGAGLAAAQEAPSPAQAAPAPAQAAP
                     ASAAAPSAAGNALLPSILFVLESGRSMGGKLGDTPKLDIARTGIEQALTNQAGRAAFG
                     VITFGHRKTWSCKDTELLAKPSTLTGESYETLLGGLKPKGRQAAIAAAVSDAASLAST
                     GEPLDVILVADGKDECKADVCKTADVLKQASKGLRVHVVGLNEKAEALDPLACIAEKT
                     GGKFVAATSGAAFRDGLDAVLASITNPPVPLDAVKTAEGPPLKPGETAVFKSGAQRTL
                     SVSPPKTNEPQQPVPVSFKALVTEGGAQLRSGLTWRVYSAKRTADGSFELISTHREPA
                     PTAALLPGEYLVNAAYGLSNLTKKLKVDGGQSLNETFVLNTGALALRGVLANGQECPD
                     GSVRFDILSDEADQFGKHEAVLADAKPRSIIRLNAGVYRIRSLYGDANAVVGVDVTVE
                     PGKVTQATLKHSGAKITFRLVQSLGGEALADTKWTILTSAGDTVKSNAGALPTHILAA
                     GNYAVVADHAGNSYTRKFSIEPGGDRQIEVAIADGPTSEEELKILLNPPDAPEPGTGI
                     AAGEGPGRAMGFDGFSRRPADPNAPLINPGTLFRPSSP"
     gene            3306..3878
                     /locus_tag="AC16Y7_06730"
     CDS             3306..3878
                     /locus_tag="AC16Y7_06730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR5709112.1"
                     /translation="MSNPTLDHLLTRRSVSANALSEPGPDESQLAQILTAAARVPDHK
                     KLVPWRFVLFQGPAREDFGRVLAEACQAEEPAASAMRLQMEGMRFLRAPVVVAVISRI
                     AKAHVVPEWEQILSAGAACQNLVVASSSMGFGAQWITEWYAYSEAVRQALGLDEAERV
                     AGFVYIGTPKEKPDERPRPELDDIVTAWQA"
     gene            3911..4507
                     /locus_tag="AC16Y7_06735"
     CDS             3911..4507
                     /locus_tag="AC16Y7_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR5709113.1"
                     /translation="MFYDAVANSHGLQWDPFKALVSPRPIGWISTLDQNGVVNLAPYS
                     FFNAVSTDPHFVMFSSGGRKDSQRNAEETGEFVCSLATFELREAMNRTSQHVGPEVDE
                     MALAGLTAAPSKIVAPPRVAESPVAFECTYWRTIELPGPDGGPGTHAIVLGQVVGVHI
                     DDAAIVDGKVDVTKLKPIARLGYGDYAVIDEVFELSRP"
     gene            complement(4519..5298)
                     /locus_tag="AC16Y7_06740"
     CDS             complement(4519..5298)
                     /locus_tag="AC16Y7_06740"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HIR5709114.1"
                     /translation="MIMPRFEKIAFVAAETTEAEDALRELSARYGNVAPEEADAIVAL
                     GGDGLMLQTMHSHLHSRIPIYGMNRGSVGFLLNDYRENQLQERLEAAEITVIHPLRMN
                     VRDVNRQTHEALAINEVSLFRQTHQAAKLRIAVDGTNRLDELICDGVLVSTPAGSTAY
                     NLSAHGPILPITAPLLALTPISPFRPRRWRGALLPNQARVQIEVLEPDKRPVSAVADH
                     TEFRDVFAVEVEEQSDVDIFMMFDPGHTLAERILAEQFGIE"
     gene            5382..5732
                     /gene="cutA"
                     /locus_tag="AC16Y7_06745"
     CDS             5382..5732
                     /gene="cutA"
                     /locus_tag="AC16Y7_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HIR5709115.1"
                     /translation="MGTPMENADRPVLIYATFPTLEEAKRVGSTLVSQRLAACVNVFP
                     GMISIYQWKGAQAEDNEVAMIIKTRAGLADAVMTETKKQHPHEVPALLVLPTEGGSDD
                     YCAWIMSETKDGGV"
     gene            5729..7220
                     /gene="glpK"
                     /locus_tag="AC16Y7_06750"
                     /pseudo
     CDS             5729..7220
                     /gene="glpK"
                     /locus_tag="AC16Y7_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191892.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
     gene            7480..8115
                     /locus_tag="AC16Y7_06755"
     CDS             7480..8115
                     /locus_tag="AC16Y7_06755"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HIR5709116.1"
                     /translation="MPTLVAAYAKGFYPEDGKWWAPAERMVSFPENAHVSKRVWRLLQ
                     TRTFGVSFDTDFAGVLRACGPEPALAEAFQALFEDGYAHSVEVWDRSGRLTGGLFGLA
                     IGQAFFTEKTFSRERDAANVGAVTLSCHLQHWGFALNDGKRMSGQLSQLGFQVVPRFA
                     FNGLLSKATLEPSRPGPWTVETGLDPARWNPKVPGTGKAAPGSRNAFTPSA"
     gene            8235..10460
                     /locus_tag="AC16Y7_06760"
     CDS             8235..10460
                     /locus_tag="AC16Y7_06760"
                     /inference="COORDINATES: protein motif:HMM:NF013109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902358 - sulfate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR5709117.1"
                     /translation="MSLLANLASKRPRNIEASTLGRDALAGTISAIVNIAYCISFSAL
                     IFQGSIAAGFPLGLAALIMGTVVTGVVVALTTTLVPADAGPDTPAVAVMSVLAATVAA
                     GLASKGASEEAMVINVLVAISVSTFLTGALLFGVGALKLGQWLRFVPYPVIGGFLAAS
                     GWLLITGGVEVITGSNLTIQPSSWAAIWDPRYAPQIGVGLAFALFIVLLKRWMDDFLT
                     LPVAFFGFLVVMNLVLFGFVTDEATRSQWFLKAVGALQLWWPLEAIATHDIDWGVLAM
                     SSVEIGAVCGVTAISMLLDVSSLEVARQKSANLDKEFQTNGLANVLASVLGGVAGNLS
                     LNNSLLLQQAGAVTRISGVSVAIVCGLVLFAGADIGAFVPKAILGGMLAYLGVMILIE
                     ALVRSPAQRSITDLALAIAIAIVIVYFGYLLGVVLGVIGACLLFALSYSRIGVIRRHL
                     TRYELSSNVERSPEQTRLLHEEGKRVHVFWLSGFIFFGSSNGLFERIKRVIEGQDEKP
                     VGYIVLDFSAVQGLDTSAVLSLVKLRNYCEEKDVTLVFSGLSDAMDAAFNGAGFFGAD
                     RPHQVFGTRNEAIEWCEDMLLLYHEVGDASTHSFESWLHHEFDGLISFDRIAQFMERQ
                     ELETGALLFKQGEAADSVVFQESGGVAITINDEYGRSIRLRRMIGHTVMGEMGFYRDM
                     PRTADVVAEAPTVVYRLTREAFDRMQEKDPVAAAALHKLIIRLLSDRLDFANREISAL
                     L"
     gene            complement(10457..10849)
                     /locus_tag="AC16Y7_06765"
     CDS             complement(10457..10849)
                     /locus_tag="AC16Y7_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HIR5709118.1"
                     /translation="MPDLAIIILAAVSVLMLKHTAADFFLQTPHQYLNKGIYGHPGGL
                     LHAGIHVVLTPLVYLVIAPASLLIALWIALGEFAVHYHVDWAKEQITRRNGLNPQVSG
                     YWHTLGIDQLLHGLTYITIVAVLVRAAS"
     gene            complement(10870..13083)
                     /locus_tag="AC16Y7_06770"
     CDS             complement(10870..13083)
                     /locus_tag="AC16Y7_06770"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HIR5709119.1"
                     /translation="MGPRLLYSLLIGAVIVASLALRVWDPAPVARMRSLVFDAFQRVD
                     PLKFDPTLPVRIVDIDEESLKRIGQWPWPRTIFAKLIGTLSEDGAAAIGFDMVFPEPD
                     RLSPSNAVQHWPQTALVAEIKPELEKLPSNDQVLAEAIATAPVVLGFIASPHTDVLPQ
                     TKAGLAHGGDDPRLFAPYYPGSAVTLKELQDPAVGAGALNWIPEFDQIIRRMPMVVRI
                     GDTLYPSFAADLLRQAQGASTYVIKSSGASGEQAFGEKTGIVKVRVGDFEVPTEASGQ
                     MWMRFTPQNRDRYLPAWKVLDGEIPAEEIAGRIILIGTSAAGLLDLRATPIEASVPGI
                     ELHAQAVEQILKGAFLQRPDFATPAELLYILLLGTLIAVLIYRAGALGSAVVSIIAIA
                     FVIGFSWYAFDKLGWLVDPVYPTIALAAIYLTGTSYVFLRTERERNRVRNAFSHYMAP
                     ALVERLADEPDRLKLGGETRDMTLLFSDVRGFTTISEGLDAEELTRFLNNLFTPLSNI
                     ILDEDGTIDKFMGDAVMAFWNAPLDDPDHPSHGCSAALRIMDEMKTLNERWQQEAEAD
                     GRVFKEVRLGIGVNTGMCCVGNLGSETRFDYSVIGDNVNVASRLEGQSKTYGFMTVVG
                     EETASRTPDFAFLELDLLKVKGKTAATRIFALLGDAAMKESQSFIELTKTHEDFLSRY
                     RAADWDAAEILSHTCEAMDSGKLETLYTLYRERIAAFRITPPPAEWDGSEEALSK"
     gene            complement(13215..14669)
                     /locus_tag="AC16Y7_06775"
     CDS             complement(13215..14669)
                     /locus_tag="AC16Y7_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HIR5709120.1"
                     /translation="MLKRFATGLIGLTLLVAALSPASAEKRVALVIGNSAYKNTTPLK
                     NPSNDAVDMAEALRALDFEVIDGTDLRKRDMMKRIRAFAEKLDGADVGLFFYAGHGLQ
                     VDGRNFLAPVDAALKSDTDLDFEAIELNLVLKQLERNARLSIVFLDACRDNPLARNLV
                     VAGRSLAVGRGLAQVEKAVGMMIAFATQPGNIALDGEGRNSPFTKALLQHIGAEGASI
                     NDVMIDVRRDVLEETKGKQVPWENSSLTGQFYFKPTATSGGGTAEIAALRDEIARLKE
                     DRSPAEAGENVTAEQADDTSAREIAVEPAGAGEAKTAETASPETKTANLEPAALTDTP
                     SQESEDKSVPVQNDPAGDQAALNRDIQEKLKALNCYTGRIVGDWGRGTRGAVKRFNIH
                     AKTDLKPGEAEPETLEALDAWTGAPCPVTVVRPKAPAPKTAKPKTRAKPKTYAKPKTA
                     AKPRHTQKPAYKSKQKPSNDFATDAVHKSLRPAR"
     gene            complement(14779..15033)
                     /locus_tag="AC16Y7_06780"
     CDS             complement(14779..15033)
                     /locus_tag="AC16Y7_06780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709121.1"
                     /translation="MTKQLIVPILGLAALATAATPAAADRGRAYVVKPAPKYIYEYDA
                     RPSKRAVTVEPRRYKRKFNPNLDFETEAVWRNLRPNWFDD"
     gene            15398..15925
                     /locus_tag="AC16Y7_06785"
     CDS             15398..15925
                     /locus_tag="AC16Y7_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709122.1"
                     /translation="MRLFNLVLATFALAGLWMVDGEAQAGRFMPEIENPYCTVKTYVL
                     RNVAELASSMTDRRGRPVIVVNRRTLRDDPAYSKFLLAHECCHHTLGHVTEFKKGLGN
                     VGPQAFLYIAPQLKRLELEADCCAVKLMRERGHADGIEAGREAMSMFGTKPTGAHYPT
                     GVERAETIMGCAVAR"
     gene            16035..16910
                     /locus_tag="AC16Y7_06790"
     CDS             16035..16910
                     /locus_tag="AC16Y7_06790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated putative
                     ABC transporter substrate-binding protein"
                     /protein_id="HIR5709123.1"
                     /translation="MKGSTTIVRRVVQSLRHPGLVASVLAATAMLCVAQSAPLAAAPV
                     LRVCADPDNLPFSNDKGEGFENKLAVLLAERLGQELEYTWSSEDSGDVSNTVGEGACD
                     LVMGYAQGTGLIEDTNPYYYTSYVLITRADDPDLKDVETLSDPQLRGKRIGLFAQTPP
                     ASILAMHGLVSNVKPFEVNPGESSARAAEAVIGEIASGELDAALLWGPIGGYHAAQTK
                     VPLRVVPLVKESAGPNTIYPITMGVRPNEPQWKHQINKLLADSEQEINVILREYNVPV
                     LNQKGERIAAGAALR"
     gene            complement(16912..18210)
                     /locus_tag="AC16Y7_06795"
     CDS             complement(16912..18210)
                     /locus_tag="AC16Y7_06795"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5709124.1"
                     /translation="MQIIWASKPLPRLVLTAALIFSGTMCVPAWAGHPSKEREGGVSL
                     QMPQSPEMMPVEIGYIREDTGRPRPASRLDAEPDDAGIAGAKMGIGENNAGGRFMGHF
                     YALDVEEASSPEDALAAIKKLHDSKHNYIIVDASAPTLLKLADWAKDKDILLFNVRAT
                     DVSLRQEGCRANVMHIVPDRYMLADALAQYLVKMNWKNWLVVHGSTAADRANTDAVKR
                     AAKRFGANIVDVREYVDVSGGRRDGVGPIPPAKPRMEASADDSVIPATGYDVIMVADE
                     DQQFGPLMPYRGGGQPRIVAGTTGLFATTWSPGHEKWGATQANNNFEKDFDRIMLPID
                     HQAYVAARTIGEAVTRKPENDFATVSAFIHGPDLQLAPFKGIKQQFRPWDGQFRQPIL
                     IATEKVPVSVSPQRGFPHASHPEIEVDTLGIDEPESTCKM"
     gene            18465..19124
                     /locus_tag="AC16Y7_06800"
     CDS             18465..19124
                     /locus_tag="AC16Y7_06800"
                     /inference="COORDINATES: protein motif:HMM:NF051612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7847 domain-containing protein"
                     /protein_id="HIR5709125.1"
                     /translation="MQFSPGDAIRYGWEAFKRRPWFFVGASFIIAIVYMLAGGISSAV
                     DGAMGGGAEEPTVLGSIVNYGLGTLISMGVTAFYLAAHDNPDTAEFSMLWHPQPFLKF
                     LGASVLTTLAIVIGLALFIVPGIIAMLLFMFSTFIVIDRGLGPIEAMKESMRITAGNR
                     WPLLGFICLLLLILMAGILALGVGLLVAMPIATLAFAHAYRLLSGGPGQAAPADARLG
                     A"
     gene            complement(19199..20431)
                     /gene="pqqE"
                     /locus_tag="AC16Y7_06805"
     CDS             complement(19199..20431)
                     /gene="pqqE"
                     /locus_tag="AC16Y7_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HIR5709126.1"
                     /translation="MTEDLRIDGHIQGIAVDDEPTTGVVGNEDEAAELCALAPVGLLA
                     ELTHRCPLQCPYCSNPVELEGAKNELTTEEWCSVMRQAGEMGILQVHLSGGEPCARKD
                     LEEIVKAASEAGLYTNLITAGVTLKRDRLETLQKLGLDHVQVSFQDVDPAGADKIGAY
                     PNALQKKLDVARWVTELGMPLTVNAPIHRHNIHNVPRYIDMSVELGAQRLEVAHAQYY
                     GWAFLNRAALIPTYEDTVASIEYVEAARERLKGVLTIDMVVPDYYAKRPKPCMGGWGK
                     GFMNITPAGKVLPCHAAESIPELEFDNARDRKLVDIWMNGDAFNAFRGTEWMKEPCRS
                     CAFKEVDYGGCRCQAMAITKDPRNTDPACALSPYHAEMVKLAKDEAAKPATEFIYRNP
                     RNAAEAGKAADEPLVPAE"
     gene            complement(20428..20733)
                     /gene="pqqD"
                     /locus_tag="AC16Y7_06810"
     CDS             complement(20428..20733)
                     /gene="pqqD"
                     /locus_tag="AC16Y7_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="HIR5709127.1"
                     /translation="MTNKTETPGRLIVSRESKPQMPPYLKLRHDAGRGRWVLLAPERI
                     LTPDQIAVEVLQLCDGKRTVEDIAAKLAEEYSAPIDVIAKDVVELLQDLADKGYIKA"
     gene            complement(20730..>21049)
                     /locus_tag="AC16Y7_06815"
     CDS             complement(20730..>21049)
                     /locus_tag="AC16Y7_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiaminase II/PqqC family protein"
                     /protein_id="HIR5709128.1"
                     /translation="VASSLTELFAPSIHKARISGMLENYDFISDDVMAYFKRRLTQAP
                     RDADFALEYVKQNARTPEAQQGVLDALRFKTHVLWAQLDALHLAYVDPGMIPPGAFVP
                     EQA"
BASE COUNT         3599 a         6299 c         6959 g         4192 t
ORIGIN      
        1 gcgcatctac ggcacggcct gggcgagcga ggacgacctc aaggcctatc tccaccagct
       61 cgaagaggcc gagaagcgcg accatcggcg tctgggccgc gagatggatc tgttccattt
      121 ccaggaagag gcgccgggcg cggtgttctg gcatcccaag ggctgggcgc tgttccaggc
      181 gctgatcggc tacatgcgcc ggtgtcagca ggcctggggc tatttcgagg tgaactcgcc
      241 ggacatgatg gagcggacgc tgtgggagca gtccggccat tgggagaagt tcgccgagaa
      301 catgtacatc gccgagacgc ccgacgaccg ggtgttctgc tgcaagccca tgaactgtcc
      361 gggccatgtg cagatcttta agaacggcct caagagctat cgcgatcttc ctttgcgcat
      421 tgcagaattc ggcaaggtgc atcgctacga gccgtccggc gcgttgcatg ggctcttgcg
      481 cgtgcgccac ttcacgcaag acgacgcgca catcttctgc acggaagatc agatcaccga
      541 ggagtgcgtg aagctcaatg cgcagatgct ttcgatttat cgtgacttcg gcttcgagga
      601 cgtgcgcatc aagttctccg acaggccgga gaagcgcgtc ggcgacgaca aggtgtggga
      661 tgcggcggaa gctgcattga tgcgcgcgat ggaagcggcc gggctcgatt acgagctgaa
      721 cccgggcgag ggcgcattct acgggccgaa gctggaatat gtcctacgcg acgccattgg
      781 ccgcgattgg caatgcggca cggtccaggt ggatctcagc atgccggagc ggctcggcgc
      841 gttctatatc ggcgcggatg gcgagaaggc tgtgccggtg atgatccacc gcgccatgtt
      901 cggttcgctg gagcgcttca cgggcattct catcgaacac catgccggcc acctgccgct
      961 ctggcttgcg cccacccaag cggtggtcgc caccatcgtg tcggacgcag acgcgtatgc
     1021 ggaacaggtc ttggcgcggc tgagaggcgc ggggctacgc gccgagacgg atctgcgcaa
     1081 cgagaagatc aactacaagg tgcgcgagca ttcggtcgcc aaggtgccgg ttatgctggt
     1141 ggtcggcaaa cgcgaggcgg aggaaggcac ggtgtccgtg cgccggctcg gctcgcaaaa
     1201 gcaagaagtg ctgagcctcg acgatgtgct gaagatgctt gaggctgagg ccgtgccgcc
     1261 ggatatgcgc gaagggaaaa gcgccgccgc ttgagtctta tggcgaagag ggccggaaca
     1321 gcgtgcccgg attgatcagt ggggcgttgg gatcggcggg ccggcgcgag aaaccgtcga
     1381 agcccattgc gcggcccggg ccttcgccgg ctgcgatgcc agtgccgggc tccggggcgt
     1441 cgggcggatt gaggagaatc ttcaactcct cctccgaggt cggcccgtcc gcaatcgcca
     1501 cttcaatctg cctgtctccg cccggctcga tagagaactt gcgcgtatag ctattgccgg
     1561 cgtggtcggc aaccaccgcg tagtttccag cggcaaggat atgggtcggc agcgcgcccg
     1621 cgttcgactt caccgtatcg ccggccgagg tgaggatcgt ccacttggta tcggccaaag
     1681 cctcgccgcc caaactttgg accagacgaa acgtaatctt cgcgccggaa tgcttgagtg
     1741 tcgcctgggt gaccttgccg ggctcgaccg tcacgtcgac gccgacgacg gcgttcgcat
     1801 cgccgtaaag actccgaatg cggtatacgc ccgcgttgag cctgatgatg gagcgcggtt
     1861 tagcgtcggc caggacggct tcgtgcttgc cgaactgatc cgcctcgtcg gaaagaatgt
     1921 cgaagcggac gctgccatcg gggcattcct ggccgttagc cagcaccccc cttaacgcca
     1981 gcgcgccggt attgaggacg aacgtctcgt tgagggactg gccgccgtcg accttcagct
     2041 ttttggtgag gttggagagc ccgtaagcgg cgttgaccaa atactctccg ggcagaagcg
     2101 cggcggtggg cgcgggctcg cggtgcgtgc tgatcagctc aaagctgccg tcggcggtgc
     2161 gcttcgcact gtagacgcgc caagtaagac ccgaccgtag ctgcgcgccg ccctcggtga
     2221 ccagggcctt gaagctgacc ggaacgggct gctgcggctc gttggttttt ggcggtgaga
     2281 cggacagcgt gcgctgcgcg ccggatttga agacagcggt ctcgccgggt ttgagcggag
     2341 gaccctcggc ggtcttcacg gcatcgaggg gcacgggcgg gttagtgatg gaggccagca
     2401 ccgcatccag tccgtcccgg aatgcagcgc cgctcgtcgc ggcgacaaac ttgccgccgg
     2461 tcttttccgc gatacaggcg aggggatcga gagcttcggc tttttcgttc agccccacca
     2521 cgtgcacccg cagacccttc gatgcctgtt tgaggacatc ggcggtcttg cagacgtccg
     2581 ccttacattc gtccttgccg tccgctacca ggatgacgtc aagcggttcg cctgtgcttg
     2641 caaggcttgc cgcgtcgctg acggccgccg cgatggccgc ttgccggccc ttgggcttga
     2701 ggccgccgag aagcgtctcg tagctctccc cggtcagcgt gctgggcttg gcgagcagct
     2761 cggtgtcctt gcagctccac gttttgcgat ggccgaaggt gatcaccccg aaggcggccc
     2821 ggccggcctg attggtgagg gcctgctcga ttccggtgcg agcaatgtcg agcttaggcg
     2881 tgtcgcccaa cttgccgccc atactcctcc ccgactcaag cacgaagagg atggagggca
     2941 acaacgcatt gcccgctgcg ctgggcgctg cggcagacgc gggtgccgcc tgggcgggcg
     3001 cgggcgccgc ttgggcaggc gagggtgcct cttgggcggc cgcgaggccg gcaccgagca
     3061 tgcaagcggg caaagccaag agcttcagcg ccgcgatgcg tatcccccgc atggcgattc
     3121 tctttcgatt cagtcggctg cgaagaccgt tatacaggct ccgggcaggc ataacgcttc
     3181 gaaagttggc cgcgaatgag gccgattttt gaagctcgga gcgcctattc gacgcaaagg
     3241 acgaacattg ttggaacgcc cgtttcgcga tatgcagcat ggcattagcg gaacaccatg
     3301 aacgcatgtc aaatccgacc ctcgatcacc tcctgacccg ccgctcggtc tcggccaatg
     3361 cgctttccga gccaggtccg gacgaaagcc agctggccca gatcctgacc gcggccgcac
     3421 gggtcccgga ccacaagaag ctcgtgccct ggcgcttcgt tctgttccag ggccccgcgc
     3481 gagaggactt cggccgcgtg ctcgccgagg cctgccaagc ggaagagccg gcggccagcg
     3541 ccatgcgcct tcagatggag ggcatgcgct ttttgcgggc gcccgtggtc gtcgccgtca
     3601 tctcccgtat tgcgaaggcc catgtggtcc ctgagtggga gcagatcctg tcggcgggcg
     3661 cagcgtgcca gaatcttgtc gtggcgtcgt cgtcaatggg atttggcgcg cagtggatca
     3721 ccgagtggta tgcctatagc gaggccgtac ggcaggcgct gggtctggac gaagccgagc
     3781 gcgtcgccgg ctttgtctat atcggtacgc cgaaggagaa gcccgacgag cggccgcggc
     3841 cagaactcga cgacattgtc accgcatggc aggcttgaaa cgcaccgcgg cggccctagg
     3901 atcacgaacc atgttttatg acgcggttgc caattcgcat ggccttcagt gggacccgtt
     3961 caaggcgctg gtcagtccgc gcccgatcgg ctggatttcg acgctcgacc aaaatggcgt
     4021 cgtcaatctc gcgccctata gcttcttcaa tgccgtttcc accgatccgc actttgtcat
     4081 gttctcctca ggcggccgca aggacagcca gcgcaatgcg gaggagacgg gggagttcgt
     4141 ctgttcgttg gccacgttcg agttgcgtga ggccatgaac aggacatccc agcacgtcgg
     4201 acccgaggtg gacgagatgg cgttagccgg gctgacggcc gcgccgtcga agatcgtggc
     4261 gccgccgcgc gtggcggagt cgccggtcgc ctttgaatgt acatactggc gcacgatcga
     4321 attgcccggt cccgacggcg ggccagggac tcatgccatc gtgctcggcc aggtggtggg
     4381 cgtgcatatc gacgacgcgg caatcgtgga cggcaaggtc gacgtgacca agcttaagcc
     4441 gattgcgcgg ctgggatatg gcgactatgc ggtgatcgac gaagtcttcg agttgtcacg
     4501 gccttaaggc gccgcgcctc actcaatccc gaactgctcc gctaggatgc gctcggccag
     4561 cgtatgtccc ggatcgaaca tcataaagat gtcgacgtcg ctctgctctt ccacttcgac
     4621 ggcaaagacg tcgcggaact cggtgtgatc ggcgacagcg ctgacgggac gcttgtcggg
     4681 ttccaacact tcgatctgca ccctggcttg gtttggcaga agcgcgccac gccagcgccg
     4741 cggacggaac gggctgatgg gcgtcaaggc cagcagcggt gcggtgatgg gcaggattgg
     4801 cccatgggcg gagaggttgt aggcggtgct gccggcgggc gttgacacga ggacgccgtc
     4861 gcagatgagc tcatcgaggc ggttcgtgcc gtcgactgcg attctcagct tggccgcttg
     4921 gtgcgtttgc cggaacagcg agacctcgtt gatggccagg gcctcatggg tctggcggtt
     4981 cacgtcgcgc acgttcatac gcaagggatg gatgaccgtg atctccgctg cctccaggcg
     5041 ctcctgtagc tggttctccc gataatcgtt gagcagaaag ccgaccgagc cgcggttcat
     5101 gccgtagatc gggatacggc tatgaagatg gctgtgcatg gtctggagca tcagaccatc
     5161 gccccccagc gcgacaatgg cgtccgcctc ttcgggcgcc acattgccgt agcgcgcgct
     5221 gagctccctt agcgcgtctt ccgcctccgt cgtctcggcg gcaacgaagg caattttctc
     5281 aaagcgtggc atgatcattg tcgctgcact tggatcggcg tgttcagggg cactataaag
     5341 ctgtgctgcc tatgtcttgc aagtgtctcg aagaacggtg aatggggacc cccatggaga
     5401 acgcggaccg gccggttttg atttatgcga cctttccgac gcttgaggag gccaagcgcg
     5461 tcgggagcac gctggtttcc cagcggctcg cggcttgcgt gaacgtcttt cccggcatga
     5521 tctcgatcta tcagtggaag ggcgcgcagg cagaggacaa cgaggtcgcc atgatcatca
     5581 agacgcgggc gggcctcgcc gatgcggtca tgaccgagac caagaagcag caccctcacg
     5641 aggtgccggc gctcctcgtt ctgccgacgg agggcggcag cgacgactat tgcgcttgga
     5701 tcatgagcga gacgaaagac ggaggcgtct gatgggctat gtgctcgcca tcgatcaagg
     5761 aaccgcgagc acgcgccatc ctgttcgacg aggccgggcg tcccggtcct acggccagtg
     5821 tgccgctgag gcagatctac cccgcaccag ggcaggtgga gcacgacgcg aacgagatct
     5881 gggacgctgt cgttgaggtc tgccgcgcgg tgttgcgtga agcgggtgcg gacgagatcg
     5941 ccgccatcgg tatcaccaat cagcgcgaga cgacggtggt gtgggaccgc gcgacgggag
     6001 agcctctcgc caacgccatc gtttggcagg atcggcgcac ggccccgctc tgcgccgagt
     6061 tggagcagga agggtggcgc gcgcatgtcg ccgaagcgac aggcctcatc atcgatccgt
     6121 atttctcggc gacgaagctt tcttggctgc tctctaatgt gcccggtctg gcgaagtgcg
     6181 ccgcacgcgg tgaggtctgc ttcggcacag tcgacagcta cctcgtgttc aaactcacag
     6241 ccggcgcgct gcatgttacc gacgccacga acgcggcgcg caccatgttg ttcgatatcc
     6301 gcaccgggac atgggacgca cggttattgg agcggctcgg cattccgcgt ggcatgctgc
     6361 cggacgtgcg cgacagccaa ggagactttg gcgtgacggc gcccgacctg ttcggcgtgg
     6421 cactccccat tcgtggcgtc gccggcgatc agcaggcggc cgcgcaaggg caggtctgct
     6481 atcggccggg gatgacgaag gcgacttacg gaacggggtg cttcgtggtc gccaacaccg
     6541 gcggcgacaa ggtcgcgtcg caaacgcgca tgttgtcgac aattttgaac cagcgcagcg
     6601 gtgcacgcac ttttgcgctg gaagggtcga tcttcatggc gggtgccacc attcagtggc
     6661 tgcgcgacaa tctcggcttg atcggcagtg cagaggagac cgaggcgctg gcgcgagacg
     6721 ccgatcccaa ttccggcgtt tatcttgtgc cggccttcca agggctcggc gcgcccttct
     6781 gggatgccga agcgaaagcg gccattgtcg gcatgagccg cgccgccggc aaagcggaca
     6841 tcgtggcggc gggcctggag gcggtcgcgc tgcaaacgcg cgatctcctg accgccatgc
     6901 gccgggacat gggcgcgagc ggtatcggcg attccggaac cttgcgggtc gatggcggca
     6961 tgaccgccaa tggctgggcc atgcagcgcc ttgccgatat tctgggcgag cgggtcgcgg
     7021 tggcgccgat tcccgaaacc acggcgttgg gcgcagccta tctggcgggc caggtggtgg
     7081 gcttttatgg cgacgatgcg gcacttgccg agacctggac accggccagg gtctatgagc
     7141 cggtcatggg cgaggacgag cgggagtccc gctatgccgg ctggctggag gccgtttctc
     7201 gcgtccgcag ccgcgaatag agctaatcgg acccttggcg ttaacccgtt gggaatccct
     7261 tttcgggcaa gctcgtttgc tgtgttgccg cgttgaatgg aggctcccac ggtgttgggt
     7321 caagtttcgc gtcgccttgt ggactctggg ctttctggca agccatccgc gcttgcagtc
     7381 ctcgccaaac atttcattgg atttggcggg gggctgccgg accccgacaa agcgcttcgg
     7441 catcccgaag ggcttgcggg caagtgcaac gacttaagcg tgccgacgct tgtcgccgct
     7501 tacgcaaaag gcttctaccc ggaggacggt aagtggtggg cgcccgctga gcgcatggtg
     7561 tcgttcccgg agaacgcgca tgtgtcgaag cgcgtctggc ggctcttaca aacgcgcaca
     7621 tttggtgtga gcttcgatac ggacttcgcg ggcgtcctgc gggcgtgcgg gccggagccg
     7681 gcattggccg aggcgtttca ggccctgttc gaggacggct acgcacattc tgtggaagtc
     7741 tgggaccgct cgggccggct taccggcggt cttttcggtc tcgccattgg ccaggccttc
     7801 ttcactgaaa agacgttctc gcgggagcgc gatgccgcca atgtcggtgc cgttacgctg
     7861 agctgccatt tgcagcattg gggctttgcg ctgaacgacg gcaagcgcat gagcggtcag
     7921 ttgagccagc ttggcttcca ggtcgtgccg cgttttgcgt tcaacggact tctttccaag
     7981 gcgaccctcg agccgagccg gccagggcca tggaccgtgg aaaccggcct cgacccggcg
     8041 cgttggaacc cgaaagtccc cggaacgggg aaagcggcgc ctggctcccg gaacgccttc
     8101 acgccttctg cctagccaag ccgacatgac gcgaggttaa cctccagaaa cgcgtttatt
     8161 ttgcttgcat tgtggtcgcc cccggccatg atcgggcctg aacgcaaccc tccaactttc
     8221 tacgtattta gcccttgtcg ctgcttgcga atttggcgtc caaacgcccg cgcaatatcg
     8281 aagcctcaac gctcgggcgt gatgctttgg cggggacgat ttcggcaatc gtcaacatcg
     8341 cctactgcat ctcctttagc gcgctgatct tccagggctc gatcgcggcc ggttttccgc
     8401 tcggacttgc ggcgctgatc atgggtacgg tggtcaccgg cgtggttgtg gcgctgacca
     8461 cgacgctggt gccggcggat gccggtcctg acacgccggc cgtcgcggtc atgagcgtgt
     8521 tggcggcgac cgtcgccgca ggccttgcgt ccaaaggcgc gagcgaagag gccatggtca
     8581 taaacgtgct cgtggccatt tcggtgagca cgttcctgac tggcgcactg ctcttcggcg
     8641 tcggtgctct caagctcggc caatggctgc gcttcgtacc gtatcccgtg atcggcggct
     8701 tcctggcggc ttcgggctgg cttctcatca ctggtggcgt tgaggtcatc acgggctcca
     8761 acctcacgat ccagccttcg agctgggcgg cgatttggga tccgcgttac gcaccgcaga
     8821 tcggggtggg gctcgccttt gcgctgttca tcgtcctgtt gaagcggtgg atggacgatt
     8881 tcctgacact gcctgtcgcc ttcttcggct tcctcgtcgt gatgaacctg gtgctgttcg
     8941 gcttcgtgac cgacgaagca acgcgtagcc agtggttcct gaaggccgtg ggcgcactgc
     9001 agttatggtg gccgcttgag gctatcgcca cgcacgacat cgattggggt gtgctggcca
     9061 tgagcagcgt ggaaatcggc gcggtgtgcg gcgtcaccgc catctccatg cttctcgatg
     9121 tttcgagcct cgaagtggcg cgccaaaagt ccgccaatct cgacaaagag tttcagacaa
     9181 acggtctcgc caatgtgctg gcctccgttc ttggcggcgt ggcgggcaat ctttcgctga
     9241 acaatagtct tctgctccag caggcgggcg cggtgacccg gatcagcggc gtgtccgtcg
     9301 ccatcgtttg cgggctggtg ctgtttgctg gggccgatat cggcgccttc gtgccgaaag
     9361 cgattctggg cggcatgctc gcctatctcg gcgtgatgat cctgatcgag gcgctggtgc
     9421 gctcgccggc gcagcgctcg atcacggatc ttgctctggc catcgcgatc gccatcgtca
     9481 tcgtctattt cggctatctg cttggtgtgg tgctgggcgt gattggcgct tgtctgctct
     9541 tcgcgctgtc ctacagccgt atcggtgtaa tccggcgcca cctcacgcgt tacgaactgt
     9601 cgagcaatgt ggagcgttcg cctgaacaaa cgcgcctcct gcacgaggag ggcaagcgcg
     9661 tccatgtgtt ctggctatcg ggcttcatct tcttcggctc ctcgaacggc ctgttcgagc
     9721 gcatcaagcg cgtgatcgaa ggacaagacg aaaagccggt gggctacatc gtgctcgact
     9781 tcagcgctgt gcaggggctc gacacttccg ccgtgctgag cctcgtgaag ctgcgcaact
     9841 attgcgagga gaaggatgtc accttggtgt tctccggctt gagtgatgcg atggacgcgg
     9901 ccttcaatgg cgcgggcttt ttcggcgcgg accggccgca tcaggtgttt ggcacccgca
     9961 acgaggcaat cgaatggtgc gaggatatgc tgctcctcta tcacgaggtc ggcgatgcct
    10021 ccacgcacag cttcgagagt tggttgcacc acgagttcga cggtttgatc agcttcgacc
    10081 gcatcgcgca gttcatggag cgacaggagc tcgaaaccgg cgcgctgctg ttcaagcagg
    10141 gcgaagcggc agactcggtc gtgttccaag aatcaggcgg tgtcgccatc accatcaacg
    10201 acgagtatgg gcgctcgatc cggctgcggc gcatgatcgg gcataccgtc atgggtgaga
    10261 tgggcttcta ccgcgacatg ccgcgcacgg cggacgtggt cgccgaagca ccgacggtgg
    10321 tttaccggct gacccgcgag gctttcgacc ggatgcagga gaaggacccc gtggcggccg
    10381 ccgcgctcca taagctgatt attcgcctgc tgtccgaccg gctcgatttt gccaatcgcg
    10441 agatttcagc gctgctttaa gaagccgccc ggaccagaac ggcgacgatg gtaatgtagg
    10501 tcagcccatg caggagctgg tcgatgccta gcgtatgcca gtagccggac acttgcgggt
    10561 tgagaccgtt gcgccgcgta atctgctcct tcgcccaatc cacatggtaa tggaccgcga
    10621 actcgccgag cgcgatccac aaggcgatca gaagtgaagc cggtgcgatc acgagataga
    10681 cgagcggcgt cagcacgaca tggatgccgg cgtgaagtag gccgccggga tggccgtaaa
    10741 ttcccttgtt cagatactgg tgcggcgtct gaagaaagaa gtcggcggcc gtgtgtttca
    10801 gcatcaggac ggacacggcc gcaagaatga tgatggcgag gtctggcatg agcgttgctt
    10861 cgacaagggt tacttcgaca aggcttcttc cgaaccgtcc cactctgctg ggggcggggt
    10921 gatgcggaag gcggcgatcc gttcgcggta gagcgtatag agcgtttcga gtttgccgct
    10981 gtccatcgcc tcgcatgtgt gactgagaat ttcggctgcg tcccaatccg cggcgcgata
    11041 gcgggaaaga aagtcctcat gtgtcttggt gagctcgatg aacgactggc tttccttcat
    11101 cgcggcgtcg ccaagcaggg caaatatacg tgtggcggcg gtcttgccct tcaccttgag
    11161 caggtccagc tcgagaaatg cgaagtccgg cgtgcgcgaa gctgtctcct cgccgacaac
    11221 cgtcatgaaa ccataagtct tggactgacc ttcaagacgc gaggcgacat tcacgttgtc
    11281 gccgatgacg gaatagtcaa accgcgtctc cgatccgaga ttgccgacgc aacacatgcc
    11341 ggtatttacg ccaatgccta gccggacttc cttgaagacc cggccgtctg cttcggcctc
    11401 ctgctgccag cgttcgttca gtgttttcat ctcgtccatg atgcgcaaag ccgcgctgca
    11461 gccatggctt ggatgatccg gatcgtccag gggtgcgttc caaaacgcca tcaccgcatc
    11521 gcccatgaac ttgtcgatgg tgccgtcttc gtcgaggatg atgttgctga gcggggtgaa
    11581 caggttgttc aagaatcgcg tcagttcctc ggcgtccagg ccttcggaga tcgtggtgaa
    11641 gccgcgcacg tcgctgaaaa gaagcgtcat gtcgcgtgtt tcgcccccga gcttgaggcg
    11701 gtccggctcg tccgccagac gttcgacaag cgcgggcgcc atgtaatgcg agaacgcgtt
    11761 gcgcacgcgg ttgcgctctc gttcggtccg cagaaagacg tatgacgtgc cagtgaggta
    11821 gatggccgca agagcgatgg tcggatagac ggggtcgacc agccagccca atttgtcgaa
    11881 cgcgtaccac gagaagccaa tgacgaaagc gatggcgatg atgctgacga cggcgctgcc
    11941 gagcgcgccc gctctataga tgagcacggc aatcagggtg ccgagcaaaa ggatatagag
    12001 aagttcggct ggcgtcgcga aatcgggccg ctgtagaaat gcgcctttca agatttgctc
    12061 gaccgcttgc gcgtgcagtt caatcccggg caccgaggct tcgatcgggg ttgctctgag
    12121 gtcgagcaga cctgctgcgc tcgtgccgat gagaatgatg cggccagcga tttcttcggc
    12181 gggaatctcg ccgtcgagaa ccttccacgc aggcaggtag cggtcgcgat tctgcggcgt
    12241 gaagcgcatc cacatctgac cgctggcttc ggtcggaacc tcgaagtctc cgacgcgcac
    12301 tttgacgatg ccggttttct cgccgaacgc ctgttcgccg ctggcgccgg acgatttgat
    12361 cacataagtg gacgcccctt gcgcctgtcg caggaggtcc gcggcgaaag acggatagag
    12421 cgtgtcgccg atgcgcacca ccataggcat gcggcggatg atctggtcga actccggaat
    12481 ccagttcagc gcgccggcgc ccactgcggg gtcctgcaat tccttcaagg tcaccgctga
    12541 gcccggatag tagggcgcga aaagacgtgg atcgtcgcca ccgtgcgcga gcccggcctt
    12601 ggtttgcggc aagacgtcgg tatgcgggga ggcgatgaag ccgagcacga cgggtgcggt
    12661 ggcgatggct tcggcaagca cttgatcgtt ggacgggagc ttctccagct ctggcttgat
    12721 ctcggcgaca agggcggtct gcggccaatg ctgtacggcg ttgctgggcg acaggcggtc
    12781 cggctcgggg aacaccatgt cgaagccgat ggccgcggcg ccgtcttcag aaagcgttcc
    12841 gataagcttc gcgaatattg tgcgcggcca cggccactgg ccaattcttt tcagcgactc
    12901 ttcgtcaata tcgacgatgc ggaccggcaa ggtggggtcg aacttgagcg ggtcgaccct
    12961 ctggaaggcg tcgaagacga gcgacctcat ccgcgcgact ggcgccgggt cccaaacccg
    13021 caaggccaaa gacgcgacga tgaccgcccc gataaggagt gaatagagaa gccgtggacc
    13081 catcccgttg cttttctgtg atttcttggc cgggcccaaa cgggcgccgg gcagacccta
    13141 tcggctggca cgggcctgtc aatgaaagga agggatggtt cgctaccgtg cctgaagatg
    13201 cggcggccgg cctctcagcg ggcgggccgg agcgatttgt gtacggcatc cgtcgcgaag
    13261 tcgttcgagg gcttctgctt cgacttgtag gccggctttt gcgtgtgtct cggcttcgcg
    13321 gctgtcttgg gctttgcgta tgtcttgggc ttcgcgcggg tcttcggctt cgccgtcttg
    13381 ggcgcagggg ccttcgggcg cacgacggtc accgggcagg gggctccggt ccatgcatcc
    13441 agcgcctcca aggtctccgg ttccgcttcg cccggcttca agtcggtctt ggcgtggatg
    13501 ttgaagcgct tcacggcgcc gcgcgtgccg cggccccaat cgccgacgat ccggccggtg
    13561 tagcagttga gcgccttgag cttttcttgg atgtcgcggt tcaaagccgc ttggtcgccg
    13621 gccgggtcgt tctgtaccgg caccgacttg tcctctgact cctggctcgg cgtatctgta
    13681 agcgccgccg gctcaaggtt cgccgtcttg gtttcagggg acgcggtttc ggccgtcttg
    13741 gcctctcccg cgccggctgg ctccacggcg atctcgcgag ccgatgtgtc gtccgcctgt
    13801 tcggcggtta cattttcgcc cgcctcggca gggctccggt cttccttcag ccgcgcgatc
    13861 tcatcgcgca gcgccgcgat ctcggcagtg cctccgccgg acgtcgcggt gggtttaaaa
    13921 tagaactggc cggtgagcga agagttctcc cacgggacct gcttgccctt ggtttcctcc
    13981 agcacgtccc ggcgcacatc gatcatcacg tcgttgatgc tcgcgccttc agcgccgatg
    14041 tgttgcagca gcgctttggt gaatgggctg tttcggcctt caccgtcgag cgcgatgttg
    14101 ccgggctggg tcgcaaacgc gatcatcatg ccgacggctt tttcgacttg ggctaatccg
    14161 cgccccactg cgagcgagcg gcccgcgacc acgagattgc gggcaagcgg gttgtcgcgg
    14221 caggcgtcga gaaacacaat cgacaggcgg gcattccgct caagctgttt gagcacgagg
    14281 ttcagctcga tcgcttcgaa gtcgagatca gtatcggact tcagcgcggc gtcgaccggc
    14341 gcgaggaagt tgcggccatc aacctggagg ccgtgaccgg catagaagaa gagtccgaca
    14401 tcggcgccgt cgagcttttc ggcgaaagcg cggatgcgtt tcatcatgtc gcgcttgcgc
    14461 aggtccgtgc cgtcgatgac ttcgaaatcg agcgcgcgga gcgcttccgc catatcgacg
    14521 gcgtcgttgc tcgggttttt gagcggcgtg gtgttcttgt aggccgagtt gccgatgacg
    14581 agcgcgacgc gcttttcggc gctggcgggg ctcagggccg cgacgagcaa ggtcaggccg
    14641 atcaagcctg tggcgaaacg cttcagcatg tgcgcctccg gatccgcctt cagcggcggc
    14701 tgtccttaga ctgacgcata attcgaggcc gataaagagc gcatgaccat cggccccgga
    14761 tgcgcgtcgt gcagtctcct agtcgtcgaa ccaattggga cgcaaattgc gccacaccgc
    14821 ctcggtctcg aagtcgaggt tcgggttgaa tttgcgcttg tagcggcgcg gttcaaccgt
    14881 cacagcgcgc ttcgagggcc gtgcgtcgta ttcgtagata tatttcggag ctggcttcac
    14941 cacataggcc cggcctctat cagccgcggc aggggtcgcg gcggtggcca gagcggcgag
    15001 gccaagaatg ggaacgatga gctgctttgt cattgcaacc tccgggtttc agagtgcgcc
    15061 gacagccaat cgcggcatag ctcatgtggg gagcctgaag cacgcaatca acgacggcct
    15121 tctttcgcct ttggaaagca aggggggcga gccttccggg cgcttatcct tgcgtttgta
    15181 cccggcgcca catgaatggg gcctgaatgt tggggagcgc tcctgctgtt tcgtgactga
    15241 ggtcaccgac cctaaccatc cgataacgca gtatttatcc ccggggtgaa gcgggcggcc
    15301 taggatggtg cgtatgaacg gggttcaaaa ttcactttgg cggcgctcgt tcccggcctt
    15361 agtctttctt cgtggggctt tgctacggga gccgattttg cgtcttttca acctcgtgct
    15421 tgccaccttc gcgcttgccg gcctttggat ggttgacggc gaggcgcaag ccggccgctt
    15481 catgcctgag attgagaacc cttactgcac cgtcaaaacc tacgtgctgc gtaatgtggc
    15541 cgagctggcg tcgtcgatga cggatcgccg ggggcggccc gtgatcgtgg tcaatcggcg
    15601 aaccctgcgc gacgacccgg cctacagcaa gttcctgctg gcgcatgagt gttgccacca
    15661 tacgctcggg cacgtgacgg agttcaagaa ggggctcggc aatgtgggac cgcaggcctt
    15721 cctctatatt gcgccgcagt tgaagcgctt ggagttggag gccgactgct gcgcggtcaa
    15781 gctcatgcgc gagcgcgggc atgccgatgg catcgaggct ggccgcgagg ccatgtccat
    15841 gttcggcacg aagccgacgg gcgcgcatta ccccacgggg gtcgagcgtg cggagaccat
    15901 catgggctgc gcggtcgcgc gataggactg gcgggacgcg cctgaaatcg ccatatcttt
    15961 ttgcaagatt gcggtttgtg ccgcgtttga gctcggcaat agttggttgg ttcggagatg
    16021 acgaagaggt ggccttgaag ggcagcacaa cgatagtccg ccgggtggtg cagagcctcc
    16081 ggcaccccgg cttggtggcc tctgtcttgg ccgccacggc gatgttgtgc gtcgctcaga
    16141 gcgcgccgct tgctgccgcg ccggtacttc gcgtgtgcgc cgacccagac aacttgccgt
    16201 tctccaacga caagggcgag ggattcgaaa acaagcttgc cgttctcctc gccgagcgcc
    16261 tcggtcagga gttggaatac acgtggtcgt ccgaagactc tggcgacgta tccaacacgg
    16321 tgggcgaggg agcctgcgac ctggtgatgg gatatgcgca aggcacgggg ctgatcgaag
    16381 acaccaaccc ctattactat acatcctatg tactgattac gcgcgcggac gacccggact
    16441 tgaaagacgt ggagacactg tcggatccgc agctccgcgg gaagcgcatc ggcctgttcg
    16501 cgcaaacgcc gccggcaagc atcctggcca tgcacggctt ggtctcgaac gtgaagccct
    16561 tcgaggtcaa tccgggcgag agttctgccc gggcggctga agcggtgatt ggtgagatcg
    16621 catcgggcga gctcgacgcg gcgctgttgt gggggccgat cggcggatac cacgccgcgc
    16681 agaccaaggt gccgctgcgc gtcgtgccgc tcgtcaagga gagcgcgggc ccgaacacga
    16741 tctaccccat cacgatgggt gtccgcccga acgagccgca atggaagcat cagatcaaca
    16801 aactgctggc cgacagcgag caggagatca atgtgatcct gcgggagtac aacgtcccgg
    16861 ttctcaacca gaagggcgag aggatcgcgg cgggggccgc gctccgctag ctcacatttt
    16921 gcaggtgctc tccggctcgt cgatgccgag cgtatcgact tcgatttccg gatggctcgc
    16981 gtgcgggaaa ccgcgctgcg gagagacgga gaccgggacc ttctcggtgg cgatcagtat
    17041 cggctggcgg aactgaccgt cccaggggcg gaattgctgc ttgatgccct tgaagggcgc
    17101 gagctgcagg tcgggcccgt ggatgaaggc cgacacggtt gcaaagtcat tctcgggctt
    17161 tcgggtcaca gcctcgccaa tcgtgcgcgc cgcgacatag gcctggtggt cgatcggcag
    17221 catgatccga tcgaaatctt tctcaaaatt attgttcgct tgggtggcgc cccatttttc
    17281 gtgtccggga ctccacgtcg tggcgaagag gccggtcgtg ccggccacga tgcgcggctg
    17341 tccgccgcca cgatagggca tgagtggacc gaactgctgg tcctcgtccg ccaccataat
    17401 gacgtcatag ccggtggccg ggataaccga gtcgtcggcg ctggcttcca tgcgcggctt
    17461 tgccggcggg atagggccca cgccgtcgcg gcgtccgccc gaaacatcca catattcccg
    17521 aacgtccacg atgttcgcgc cgaaccgttt cgccgcccgc ttgacggcgt cggtgttggc
    17581 gcggtcggca gcggtcgagc cgtggacgac gagccaattc ttccagttca tcttgacgag
    17641 atactgcgcc agggcgtcgg ccagcatgta gcggtccggc acgatatgca tgacgttggc
    17701 gcggcaacct tcctgcctga gcgagacgtc ggttgcccgg acgttgaaca gcagaatgtc
    17761 cttgtccttt gcccagtcgg cgagcttgag cagcgttggc gcggacgcgt ccacgatgat
    17821 gtagttgtgc ttgctgtcgt ggagtttctt gatcgcagcg agcgcgtctt ccggcgatga
    17881 cgcctcctcg acatcgagcg catagaaatg gcccatgaag cggccgcctg cattgttctc
    17941 tccgatgccc atcttggcgc cggcaatccc ggcatcgtcg ggctccgcat ccaggcgtga
    18001 ggccggccgc ggccgaccag tgtcctcacg gatgtagccg atctcgaccg gcatcatctc
    18061 gggtgactga ggcatttgga ggctgacgcc gccctcgcgc tctttcgagg ggtgaccggc
    18121 ccaggcaggc acgcacatcg tgccgctgaa aatgagcgcg gcggtcagca cgaggcgggg
    18181 gaggggcttc gaagcccaga tgatctgcat gacgagaccg ttggcgccgg ttaagcggtg
    18241 aatccgaacc cgagtttggc cctcgccggc gggttccaga gggccagccg agtgttggcc
    18301 ggcatggtac cgacaagaaa atgggcattg caaggcggaa acttcggcag gaataattgc
    18361 caaagggaaa tgccatcgcc cttgaggttc ggagttttgt gcgcgcctgt gggcgaaatc
    18421 cgttgttgct ccccgaatcg tgaggggatt gctatcgtcc gcccatgcag ttctcgcccg
    18481 gagatgccat ccgctatgga tgggaggcat tcaagagacg cccgtggttc ttcgtgggcg
    18541 cgagctttat catcgcaatc gtgtacatgc ttgccggcgg gatcagttcg gctgtcgacg
    18601 gcgcaatggg cggcggcgcg gaagagccca ccgtcctcgg ctccatcgtc aactacggtc
    18661 tcggtacgct catcagcatg ggcgtgacgg cattttatct ggccgcgcac gacaaccccg
    18721 atacggcaga gttctccatg ctgtggcacc cgcaaccgtt cttgaagttc ctcggtgctt
    18781 cggtcctgac aacgctggcc atcgtcatcg gccttgcctt gttcatcgtg cccgggatca
    18841 tcgccatgct gctgttcatg ttctcgacct ttatcgtgat cgaccgtggg cttggcccca
    18901 tcgaggcgat gaaggaaagc atgcgcatca cggccggtaa tcgctggccg ctgctcgggt
    18961 ttatctgcct gttgttactc attctcatgg ccgggatttt ggcccttggg gttgggcttc
    19021 tcgtcgccat gccgatcgcg acgctcgcct tcgcgcacgc ttatcgcctg ttgtcgggcg
    19081 ggcccggtca agcagcaccg gctgatgcgc ggctcggcgc ctaaggggcc gatctcctga
    19141 aacaaaaagc gccccgcgat gggggcgcct tctgaaaatc gctctgagtt tgtgacgtct
    19201 actcggcggg aacgaggggc tcgtcggctg cctttccggc ctcagcggcg ttgcgcggat
    19261 tacggtagat gaactcggtc gcaggcttgg cggcctcatc cttggcgagc ttcaccatct
    19321 cggcgtgata gggcgacagc gcgcaagccg gatcggtgtt ccgcgggtcc ttggtgatgg
    19381 ccatggcctg gcagcgacag ccgccatagt cgacctcttt gaacgcgcag gagcggcacg
    19441 gctccttcat ccactcggtg ccgcggaagg cgttgaacgc atcgccgttc atccagatgt
    19501 cgacaagctt cctgtcgcgc gcattgtcga actccagctc cgggatgctc tccgcggcgt
    19561 ggcagggcag caccttgccc gccggcgtaa tgttcataaa gcccttaccc cagccgccca
    19621 tgcagggctt cggccgcttg gcgtagtagt cgggcacgac catatcgatg gtgagcacgc
    19681 ctttcaggcg ctcgcgcgcg gcctcgacat attcaatgga ggccactgtg tcttcgtagg
    19741 tgggaatgag cgcagcgcgg ttgaggaagg cccaaccgta atactgcgca tgggccacct
    19801 ctaaacgctg agcgcccagt tcgacggaca tgtcgatata gcgcggcacg ttatgaatgt
    19861 tgtggcggtg gatcggcgcg ttgaccgtca acggcatgcc gagctccgtc acccaacggg
    19921 cgacgtccag tttcttctgc agggcgttcg gataggcgcc aatcttatcc gcgcctgccg
    19981 ggtcgacgtc ctggaacgag acctggacat gatcgaggcc aagcttctgc agggtttcga
    20041 gccggtcgcg ctttaaggtg acgccggcag tgatgaggtt cgtgtagagc ccggcttcgg
    20101 aggccgcctt gacgatctcc tccaggtcct tgcgcgcgca cggctcgccg ccggacagat
    20161 gcacttggag gatgcccatc tcgcccgcct ggcgcatgac ggagcaccac tcctcggtgg
    20221 tgagttcgtt cttggcgcct tcgagttcga cggggttaga gcaataagga cattgcagcg
    20281 gacagcggtg cgtaagctcg gcaagcaggc caaccggtgc aagggcgcac agctcggccg
    20341 cctcgtcttc gttgccgaca acgcccgtcg tcggttcgtc gtcgacggcg atgccttgaa
    20401 tgtgtccgtc tatcctaaga tcctcggtca tgcctttatg tatcccttgt cggcaaggtc
    20461 ttggagcagc tccacgacgt ctttcgcgat cacgtcgatg ggggcggaat actcctcggc
    20521 cagcttcgcg gcgatgtcct ccacggttct ttttccgtcg cagagctgca gcacctcgac
    20581 ggcaatttga tccggcgtca gaatccgctc cggcgccaga agcacccatc gcccgcgccc
    20641 cgcgtcatgg cgaagcttca ggtagggtgg catttgcggt ttcgactccc ggctgacaat
    20701 cagccggccg ggagtttccg ttttattcgt catgcctgtt ccggaacgaa agcgcccggc
    20761 gggatcatgc ccggatcgac ataggccaga tggagcgcat cgagctgcgc ccacagcaca
    20821 tgggtcttga agcggagcgc atcgagcacg ccctgttgcg cctcgggggt gcgcgcgttc
    20881 tgcttcacat attcgagggc gaagtcggca tcgcgcggtg cctgggtgag gcggcgcttg
    20941 aaatacgcca tcacgtcgtc gctgatgaaa tcgtagttct cgagcatgcc ggagatgcgc
    21001 gccttgtgga tgctcggcgc gaacagctcg gtgagcgagg acgccacag
//
LOCUS       DBNHVU010000166         2842 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_803416, whole genome shotgun sequence.
ACCESSION   DBNHVU010000166 DBNHVU010000000
VERSION     DBNHVU010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_803416"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="AC16Y7_06820"
     CDS             complement(<1..700)
                     /locus_tag="AC16Y7_06820"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HIR5709129.1"
                     /translation="MPPLEEDYELLKSAVRKAGALANSYFTKELEVHKKPDGSEVSEA
                     DLAVDAALKLDLTTRRKTYGWLSEESPDDRERLKHSRVWMIDPIDGTSAFLRHIPEWT
                     VSAALIEDGEPVLGVVYNPAKDEFFHAMRGHGAFLNDAPIHVSDKASLEGAKMIASGG
                     LFRKKIWEEPWPNVEAEWVNSVAYRMALVAAGKGDATISLSPKAEWDLAGAALLIEEA
                     GGITTDHRGTPHPYN"
     gene            complement(999..1952)
                     /gene="ubiA"
                     /locus_tag="AC16Y7_06825"
     CDS             complement(999..1952)
                     /gene="ubiA"
                     /locus_tag="AC16Y7_06825"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HIR5709130.1"
                     /translation="MPRPTPQGKASGVADAEPKNWVDRHAPAWALPYLRLARADRPVG
                     YFLLALPCFWSVALAARSAGAPYPDPWLLLLFAIGAIAMRSAGCTYNDIVDRKIDAQV
                     ARTRSRPLPSGQVSVAAAAIFMVALCLVGLGVLLSLNHFSILLGLGVIPIVALYPFVK
                     RISHWPQAVLGLAFNWGALVGWAAVLGGLTVAPVILYVGAVAWTIGYDTIYAHQDRED
                     DALIGMKSTALRFRRSTQTWLSAFYGLAWSAIVLSGLAAGAGPVFVAGMGLAGAQLAW
                     QVATLDIDDAENCLRRFRSNRGFGLLIFVAITLDMALASWA"
     gene            1995..2726
                     /locus_tag="AC16Y7_06830"
     CDS             1995..2726
                     /locus_tag="AC16Y7_06830"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007009692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIR5709131.1"
                     /translation="MPSKLQRLFVKSPLRAGAGIALEGPYAHYLANVLRLKAGDQVLV
                     FNGLDGEWCARLAKVAKKSVMLQAEHQTRPQEAGPDLHYLFAPIKRARVDYMAQKATE
                     MGASLLQPVLTRRTVAERVKVERLEANAVEAAEQCGILRVPEVCAPKKLDKLLKDWDE
                     ERLLIFADEAAVSASPLDVLAAQSPAPLAVLIGPEGGFEPEERAALLRQPFVLPVSLG
                     PRIMRADTAAMACLALVNAVLGDWR"
BASE COUNT          517 a          900 c          900 g          525 t
ORIGIN      
        1 ggttataggg gtgcggcgtc ccgcgatggt cggtggtgat gccgccggcc tcctcgatga
       61 gcaaggccgc cccggccagg tcccactcgg ccttgggcga cagcgagatg gtcgcgtcgc
      121 ccttgccggc tgccaccagc gccatgcggt aggcgaccga gttcacccat tcggcctcga
      181 cattcggcca gggctcttcc cagatcttct tccggaacag cccgccgctg gcgatcatct
      241 tggcgccttc gaggctggcc ttgtcgctca catggatcgg cgcatcgttg aggaaggcgc
      301 cgtgtccgcg catggcatgg aagaattcgt ccttcgcggg gttgtagacc acgccgagca
      361 caggctcccc gtcctcgatg agcgcggcgg aaacggtcca ctctgggatg tggcgcaaga
      421 aggcactcgt gccgtcgatc gggtcgatca tccacacccg cgaatgtttc agccgctcgc
      481 gatcgtcggg tgattcctcc gacagccagc cgtaggtctt gcggcgcgtc gtcaggtcga
      541 gcttgagcgc ggcgtcgacg gccaggtcgg cttcgctgac ctccgagccg tcaggcttct
      601 tgtgaacttc gagctctttt gtgaaatagc tgttcgccag ggcgccggcc ttgcgcacgg
      661 cgctcttcag aagttcgtaa tcctcctcca gcggaggcat gatttcaggt ctttttgtct
      721 gtgtcgctgc ggtcatcggg cggaaggaac actctctaaa ccaagttgga agctaccggt
      781 tattaacaac gtccattaag cgtccgggaa agcttccgtg gcacattggt gtcaataaag
      841 gctacctcaa ggcagccaat aaggaagcta acagcaaggc gtcacatggg cctcggatgc
      901 gaaaagcacc ggggccctct tgctgtccgg gccgccgcgt tctcgcgtca tcccttgcat
      961 gtctcagacc acttccccaa gaccgtcaca tcggccttct aggcccaaga tgcaagcgcc
     1021 atatcgaggg tgattgccac aaatatgagc aggccgaagc cgcgatttga gcggaagcgg
     1081 cgcaggcagt tctccgcatc gtcgatatcg agtgtcgcga cctgccaggc cagttgcgcg
     1141 ccggcgagcc ccatccctgc gacaaataca gggccggccc cggccgcaag tcccgacagg
     1201 actatcgcgc tccaggccag gccgtagaaa gcgctgagcc aggtctgtgt cgaccggcgg
     1261 aagcggagcg ccgtcgattt catgccgatg agcgcgtcgt cttcgcgatc ctggtgcgcg
     1321 tagatcgtgt cgtagccgat ggtccaggcg acggcgccga cataaagaat caccggcgct
     1381 acggtgagcc cgccaagcac cgcggcccac cccaccagcg ccccccagtt gaacgcaagc
     1441 ccgagcaccg cttgcggcca atggctgatg cgcttcacga acgggtagag cgccacgatg
     1501 ggaataacgc cgagcccgag caggatcgag aagtgattga ggctgagcaa gacgccaagt
     1561 cccacgagac acagcgcgac cataaagatg gctgccgctg ccaccgaaac ctgcccgctg
     1621 ggcaaggggc gtgagcgcgt gcgcgccact tgcgcgtcga tcttgcggtc cacgatgtcg
     1681 ttataggtgc acccggcgct ccgcatggcg atggcgccga tggcaaacag gagaagcagc
     1741 cagggatccg ggtagggcgc gccagcgctg cgcgccgcaa gcgccacaga ccagaagcac
     1801 ggcagtgcta gaagaaagta gcccacgggc ctatcggcgc gggcaagccg tagatacgga
     1861 agcgcccacg ccggcgcatg gcggtccacc cagttcttgg gttccgcgtc ggcgacgccg
     1921 cttgctttgc cttgtggtgt gggacgtggc atgtctgcct tcagcctacc tcatgcatgc
     1981 gccgatcgcc gatcatgccc tctaagctcc aacggctctt cgtcaagtct ccattgcgcg
     2041 ccggcgccgg gatcgccctg gaaggcccct acgcgcatta tcttgcaaac gtgctgcgct
     2101 taaaggccgg ggaccaagtt ctcgtcttca acggcctgga tggcgagtgg tgcgcgcggc
     2161 tcgctaaggt cgcgaagaag tcagtgatgt tgcaggccga gcatcagaca cgtccgcaag
     2221 aggcaggacc tgacctgcac tatctcttcg cgcccatcaa gcgcgcgcga gtcgattaca
     2281 tggcccagaa ggcgaccgag atgggcgcca gcctattgca acccgtcctc acgcgccgca
     2341 cggtcgccga gcgagtgaag gtcgaaaggc ttgaggccaa cgcggtcgag gcggcggagc
     2401 aatgtggcat tctccgcgtg cctgaggttt gtgcgcccaa gaagctcgat aagctcttga
     2461 aagattggga cgaggagagg cttcttatct ttgccgacga ggcggcggtc tcggcttcgc
     2521 cgcttgatgt tcttgccgcg cagagcccag cgccgcttgc cgtgttgatc ggcccggaag
     2581 ggggcttcga gccggaggag cgtgccgcgc tcttgcgcca acctttcgtc cttccggtgt
     2641 ctctggggcc gcgcatcatg cgggccgaca cggccgccat ggcctgtctg gcgctcgtca
     2701 acgccgtcct cggcgattgg cgctgacatt cgtcaaatct ccgggacgat cttgagggct
     2761 ttttcacaat gacggtgctt gccgtccttt agggtcgcag aatcgttccc aaagcactaa
     2821 tttggaccgg agccggattc tg
//
LOCUS       DBNHVU010000167         6232 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1620391, whole genome shotgun sequence.
ACCESSION   DBNHVU010000167 DBNHVU010000000
VERSION     DBNHVU010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6232
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1620391"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="AC16Y7_06835"
     CDS             complement(<1..756)
                     /locus_tag="AC16Y7_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003586473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HIR5709132.1"
                     /translation="MRTDLFDFDLPEALIALRPVHPRDAARLLVVRPGARGGRSDRTV
                     TDLPTLLSPGDVLVFNDTRVIPAALKGTRVRGEAVAQISVNLTERLDGQRWLALARPA
                     KRLEPGDRIQFGGDGNACLLDALDATVEGRGEDGEVTLRFDLSGPALHDAIDAQGAMP
                     LPPYIAAKRPADEQDRVDYQTTFAREEGAVAAPTAGLHFTPALNDALEQSGIVSHFVT
                     LHVGPGTFLPVRGEDTDEHKMHAEYGSVSTDTAF"
     gene            complement(764..1219)
                     /locus_tag="AC16Y7_06840"
     CDS             complement(764..1219)
                     /locus_tag="AC16Y7_06840"
                     /inference="COORDINATES: protein motif:HMM:NF016535.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5709133.1"
                     /translation="MWVPGLLFTLVALVVGGMIALQAPINAEVATRLGHPISAATMSF
                     VVGTIMLLALALLFARGSTNLGALQTMPLYMLLGGGLLGAIYVTVNLMLAPKIGIAAI
                     MALGIAGQLFTALFLDRFGMFELTVRELSLGRVSGAILVLVGALMVRVL"
     gene            complement(1250..1711)
                     /locus_tag="AC16Y7_06845"
     CDS             complement(1250..1711)
                     /locus_tag="AC16Y7_06845"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR5709134.1"
                     /translation="MANPEDTLILETSKGNVVVEMRPDLAPGHVARIKELARQGFYDG
                     VPFHRVIEGFMAQTGDPTGTGTGGSGQKLSAEFNAEPHTRGAVSMARAQSPDSADSQF
                     FICFDDASFLDRKYTVWGRVVEGMENVDQINRGEPPSNPDKIVSAKIAADA"
     gene            complement(1739..2386)
                     /locus_tag="AC16Y7_06850"
     CDS             complement(1739..2386)
                     /locus_tag="AC16Y7_06850"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR5709135.1"
                     /translation="MTIRATVLFTAALLFTAFSATVPASAANLGNPASLNEKAPNVYK
                     AKFDTSKGTFVIEVHREWAPNGADRFYNLVKSGFYNDARFFRVIDGFMVQFGISGNPD
                     LSAVWREARIPDDQVKQSNTRGTVTFATAGPNTRTTQVFINFGDNAGLDGQGFSPFGK
                     VVSGMDVVDAFFKGYGEGAPRGRGPNQGLVQQQGNAYLITQFPKLDYIKKATIEQ"
     gene            complement(2409..2903)
                     /gene="coaD"
                     /locus_tag="AC16Y7_06855"
     CDS             complement(2409..2903)
                     /gene="coaD"
                     /locus_tag="AC16Y7_06855"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012456779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR5709136.1"
                     /translation="MRVGLYPGSFDPVTFGHMDIVRRAAQLVDRLVIAVGTHHDKHPL
                     FSAEERVALAKTVLSPIAEERGLGLNVTTYDSLTVDAARSADAAIVIRGLRDSGDFDY
                     EMQMAGMNASLAPDIETVFLASSPGTRHIAASLVRQIAAMGGDVSTFVPPIVASTLQK
                     KFNT"
     gene            complement(3107..3826)
                     /locus_tag="AC16Y7_06860"
     CDS             complement(3107..3826)
                     /locus_tag="AC16Y7_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW/AlkL family protein"
                     /protein_id="HIR5709137.1"
                     /translation="MNKFTTGFLAAAALLASAAWDVSPVMAGDSHGNFMIRGLVTGVL
                     PDTDASVTAGGARIGGANADVSDEIIPATTLTYFFNDNIAVELFCCFAKHDVTGTGSI
                     AGLGEIADTWIFPPALTAQYHFNTEGTIKPYVGAGLQYIAFFNEGTGQNRLGATKVNI
                     DDGLGFTLQAGLDISVGDGWYLNADVKKTWLNTDVNWNGTGVRADVDLDPLIISAGFG
                     YRFNLGDLFGHGAQVYETTLK"
     gene            4182..4910
                     /locus_tag="AC16Y7_06865"
     CDS             4182..4910
                     /locus_tag="AC16Y7_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024934.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR5709138.1"
                     /translation="MFGNTNARPSERTIDVSQSIPGKLATHGAVVRTRRGQSLSLALD
                     GAEMVYVVRSGALMLRVAFPDEMRQVVALLYPGEVFRAAFAPPAASARLSSVGAGEAL
                     RFRWPVFSDLAATNPEIERFYNDAVARQTARAAIHTAAVGRFDCRQRVATFLLELALR
                     TGVRAPCGGLDFEIPLNRTDIADYLGLNADTLSRTMSRLRASGLLSHPERNRGLVRDV
                     AALAALTPAARALMGLNGGGQPPA"
     gene            complement(4940..>6232)
                     /locus_tag="AC16Y7_06870"
     CDS             complement(4940..>6232)
                     /locus_tag="AC16Y7_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HIR5709139.1"
                     /translation="ADYLEILRSRARIVGIVKDELTELKKQFGTPRRTEIVDMVGEVE
                     DEDLIQREDMVVTVSHKGYIKRVPLSAYRAQRRGGKGRAGMATRDEDFVSRIFIANTH
                     TPVLFFSSGGMVYKMKVWRLPAAAPQARGKALVNLLPLSKHETITTILPLPEDESSWA
                     ELDVMFATNYGSVRRNKLSDFIEVRQNGKIAMKLDEKDHIVGVEICNPQQDVLLTSRD
                     GQAIRFPTTDVRVFKGRDSAGVRGIKLGKDDELISLTILNHIEAEASERAAYVRQANM
                     VRRGGADYEPDIEPEEGDAEAVALSTERYAELSAHEEFVLTVSENGYGKRSSAYEYRV
                     SGRGGKGIIGMVVNPRNGKLVASFPAQDSDQIMLVTNRGQLIRIPVEGISIMGRSTQG
                     VIVFDTAEDERVVSVEHIPDENGEDGVEELEDDVPGSE"
BASE COUNT         1118 a         1979 c         1966 g         1169 t
ORIGIN      
        1 aaaagccgtg tccgtgctga ccgatccgta ctccgcatgc atcttgtgct catcggtgtc
       61 ctcgccgcgc accggtaaaa acgtgcctgg tcccacatgc agcgtcacga aatgggagac
      121 gatgccagat tgctcgaggg cgtcgtttag cgcgggcgta aaatgcaggc ctgcggtggg
      181 ggcggcgacg gcgccctcct cccgcgcgaa ggtcgtttga taatccacgc ggtcctgctc
      241 gtccgcgggc cgcttggcgg cgatgtaagg cggcaacggc atggcgcctt gcgcatcgat
      301 ggcgtcgtga agcgccggtc cgctcagatc gaaacgaagc gtcacttcgc cgtcctcgcc
      361 ccgcccctcg acggtggcgt ccagcgcgtc caaaagacaa gcattgccgt ccccgccgaa
      421 ctggatgcgg tcgcccggct ccaatcgctt cgccggccgc gccagtgcca gccaacgttg
      481 cccatcgagg cgttcggtca ggttgactga gatctgcgcc acggcctctc cacgcacgcg
      541 ggtgcccttc agcgccgctg ggatcacgcg tgtatcgtta aataccagca catcgccggg
      601 cgacagcagc gtgggcaagt cggtgaccgt gcgatcggaa cgcccgccgc gagcgccggg
      661 cctgaccacc aaaagccgcg ccgcgtcgcg cggatgcacc gggcgaagag cgatcaacgc
      721 ctcgggtaaa tcaaagtcga agagatctgt gcgcaatgga gacctaaagc acccgcacca
      781 tgagcgcgcc gaccaggacg agaatggccc cgctcacgcg gcccaggctc agctcccgca
      841 ccgtcagctc gaacatgccg aagcggtcca gaaaaagcgc tgtgaacagt tgcccggcga
      901 tgccaagcgc catgatggcg gcaatgccaa tcttgggcgc gagcatgagg ttgaccgtga
      961 catagattgc acccagcagg cccccgccaa gtagcatata gagcggcatg gtctgcaacg
     1021 cgccgagatt ggtgctgccg cgcgcgaaca gaagcgccag cgcaagaagc atgatcgtgc
     1081 cgacgacgaa cgacatggtt gcggcggaaa tgggatgccc aagccgcgtc gcgacctcgg
     1141 cgttgatggg cgcctgcaaa gcgatcatcc caccgaccac aagcgcaacc agcgtgaaca
     1201 gaagacctgg cacccacatg ggcattcccc tttgttgcat gatcagcctt taggcatccg
     1261 ccgcgatctt cgccgacacg atcttgtcgg ggttggacgg cggctcgccg cgattgatct
     1321 gatcgacatt ctccatgcct tcgaccacgc ggccccagac cgtgtatttg cggtcgagaa
     1381 aactcgcgtc gtcgaagcag atgaagaact ggctgtccgc ggagtccggc gattgcgcgc
     1441 gcgccatgga taccgcgccg cgggtgtgcg gctcagcgtt gaattcagcg ctgagcttct
     1501 gcccggatcc gcccgtgccc gtgccggtcg gatcgccggt ctgggccatg aagccctcga
     1561 tcacgcgatg gaacggcacg ccgtcataga agccctgacg tgccagctcc ttgatgcgtg
     1621 ccacatggcc cggcgcaagg tctggccgca tctcgaccac gacgttgccc ttgctcgtct
     1681 ccaggatgag tgtgtcttcc ggatttgcca cgatgcctcc ttggttggcg gatttcggtt
     1741 actgctcgat ggtggccttc ttgatgtagt cgagctttgg aaactgggtg atcagatagg
     1801 cgttgccttg ctgctgaacg agcccctggt ttggaccgcg gccgcgcggc gcgccttcgc
     1861 cgtagccctt aaagaacgcg tcgaccacat ccatgccgga gacgactttg ccgaacggcg
     1921 agaaaccctg gccatcgaga ccggcattgt cgccgaagtt gatgaacacc tgcgtagtgc
     1981 gcgtgttggg cccggcggtg gcaaacgtca ccgtgccgcg cgtattgctc tgcttcacct
     2041 gatcgtcggg aatgcgcgct tcgcgccaca cggccgacag gtccggattg ccgctgatgc
     2101 cgaactgcac catgaagccg tcgatgacgc ggaagaagcg cgcgtcgttg tagaagccgc
     2161 tcttcaccag gttgtagaac cggtcggcac cgttgggcgc ccactcgcgg tgcacttcga
     2221 tgacgaacgt gcctttcgac gtgtcgaact tcgctttgta gacattcggc gccttctcgt
     2281 tgagcgaggc cggattgccg agattggcgg cgctggcggg aactgttgcg ctgaacgcgg
     2341 tgaacagcaa agcagcggtg aaaaggactg tagccctgat agtcatgagg tctcctcgcg
     2401 ggggttgttt aagtgttgaa tttcttctga agcgttgaag cgacgatggg cgggacgaag
     2461 gtcgaaacat cgccacccat tgcggcgatc tgacgaacca agcttgccgc gatatggcgt
     2521 gtgccgggcg atgaggcaag gaacacggtc tcgatatccg gcgcgagcga agcgttcatg
     2581 cccgccatct gcatctcata gtcgaaatcg ccggagtccc tcaggccgcg aatgacgatt
     2641 gcggcgtctg cgctcctcgc cgcatccacc gtcaggctgt cataggtggt gacgttgaga
     2701 ccaagcccgc gctcctcggc gatcggcgag agcaccgtct tggccagggc tacccgctct
     2761 tcggcggaaa acagcggatg cttgtcgtga tgggtgccga ccgcgatcac caggcgatcc
     2821 acgagctggg ccgcccggcg cacgatatcc atgtggccga aggtgacggg atcgaaactc
     2881 ccgggataaa gaccaacgcg catgccgcca acgctccttt gagggctcct tgcgcttcgc
     2941 agcgtcatcc cgcagtgggc gggcattgcc gaaaagcgtc tgcccccttc tagccgtgcg
     3001 ggcgcaggcc cggcaagggg acatccgccc gccgctcaag tggcgcacaa gcaaaaaggg
     3061 cccggcaagg ccggaccctt cacgctgagt ttcgtggttt tttggtctac ttcagcgttg
     3121 tctcgtagac ctgcgcgccg tggccgaaca gatcgccaag gttgaagcgg tagccgaaac
     3181 cggcggagat gatcagcgga tcgagatcca cgtccgcgcg tacgccggtg ccgttccaat
     3241 tgacgtcggt gttgagccag gtcttcttca cgtcggcatt gaggtaccag ccgtcgccca
     3301 cggaaatgtc gagacccgcc tgaagggtga aaccaagccc atcgtcaatg ttgaccttcg
     3361 ttgcgccgag ccggttttgg cccgtgccct cattgaagaa ggcgatgtat tgcaggcccg
     3421 cgcccacata aggcttgatc gtgccttcag tgttgaagtg gtactgcgcc gtcagggccg
     3481 gcgggaagat ccaggtgtcg gcgatctcgc cgaggcccgc gatcgagccg gtgcccgtca
     3541 cgtcgtgttt ggcgaagcag cagaacagct caaccgcgat attgtcattg aagaagtagg
     3601 tcagcgtcgt ggccgggatg atctcgtcgc tgacatcggc gttggcaccg ccaatgcgcg
     3661 caccgcccgc cgtgacggag gcatcggtat caggcagaac cccggtgacc agaccgcgga
     3721 tcatgaagtt cccgtggctg tccccggcca tgacgggaga cacatcccac gcagcggacg
     3781 ccagcagagc ggcggcggcc aaaaaaccag ttgtgaattt attcatatca acgtccccca
     3841 gtcgtggccg cgatccggcc cctcaccctt gttctgtcgc attgttgaaa tgaaggaggg
     3901 ccgacctcca gtatagatcg gcgtcgccgc ctgacggaag tcagccccgg tcatatcggg
     3961 ggttcactgt tgcccgcgtg caacggtttt tctcgatctg catcaaacct ttgcgggcat
     4021 tcgggagcct ggcacgcggg gtcgcggagc cacgggcctt cggggcttga tcgatatcaa
     4081 agcgcgactc gcgtcgaaga cggacagctt gctcttcgat ggcggaaggc ccgccgtggg
     4141 gatgaccctt ggtagcaaca accagactga aagagaggga ggtgttcgga aacacgaacg
     4201 cccggccctc agaacgcacg atcgacgtct cgcaatccat tcctggcaaa cttgccacgc
     4261 atggcgccgt ggtgcggact cggcggggac aatcgctgtc gcttgccttg gacggcgcgg
     4321 aaatggtcta tgtggtccgc tccggcgcgc tgatgctgcg ggtggccttc ccggacgaaa
     4381 tgcgccaggt ggtggcgctt ctgtatcctg gcgaggtgtt ccgcgccgcc ttcgcgccgc
     4441 cggccgcttc ggccaggctt tcatccgttg gcgcgggcga agccttacgg ttccgctggc
     4501 cggttttctc cgatctggcg gcgacgaacc cagagatcga gcgcttctat aacgatgccg
     4561 tggccaggca gaccgccagg gccgccatcc atacggccgc ggtgggtcgc ttcgactgcc
     4621 ggcaacgggt cgcgaccttc ctcctcgaac ttgccttacg gaccggcgta cgggcgccat
     4681 gcggcgggct cgacttcgag attccgctca accgcaccga catcgccgat tatctagggc
     4741 tcaacgccga caccctgtcc cggaccatgt cgcgcttgcg cgcctcaggg ctcctgagcc
     4801 atccggagcg caatcggggt ctcgtgcgcg acgtggccgc gctggcggcg ctgacgccgg
     4861 cggcacgggc gctgatgggg ctcaacggcg gaggccagcc accagcctga cgccctaggt
     4921 tccagggttt aggtcctggc tactcgctcc cgggtacatc gtcctcgagc tcctcgacac
     4981 catcttcgcc gttctcgtcg ggaatgtgct cgaccgacac cacgcgctcg tcttcagcgg
     5041 tatcgaacac gatgacgcct tgggtcgacc ggcccatgat ggagatgcct tccacgggaa
     5101 tgcggatcag ctggccacga ttggtgacga gcatgatctg gtcgctgtcc tgcgccggga
     5161 aggacgccac cagtttgccg ttacgcggat tgacgaccat gccgataatg cctttgccgc
     5221 cgcggcccga gacgcggtac tcgtaagcgc tcgaccgttt gccgtagccg ttctccgaaa
     5281 ccgtcaatac gaattcctcg tgcgcgctga gctccgcgta gcgctcggtg ctgagcgcca
     5341 cggcctccgc atcgccctcc tcgggctcga tgtcgggctc ataatccgcg ccgccacggc
     5401 gtaccatatt ggcttgccgc acataggcgg cccgttcgga cgcctccgcc tcgatatggt
     5461 tgaggatcgt caaagagatg agctcgtcat ctttgccgag cttgatgccg cgcacaccgg
     5521 ccgagtcccg tcctttgaac acgcgcacgt cggtggtggg gaaccggatc gcctgaccgt
     5581 cccgcgaggt gagcagcacg tcctgctgcg gattgcagat ctccacgccg acgatgtggt
     5641 ccttttcgtc gagcttcatg gcgatcttgc cgttctggcg cacctcgatg aagtccgaga
     5701 gcttgttgcg gcggacgctg ccataattgg tcgcgaacat cacgtcgagt tccgcccaag
     5761 acgactcgtc ctccggtagc ggtaaaatgg tagtgatggt ctcgtgcttg ctgagcggca
     5821 ataggttgac caacgccttg ccgcgcgctt gcggtgcggc tgcgggcaaa cgccacactt
     5881 tcattttgta gaccatgccg ccggaggaaa agaacagcac cggcgtatgg gtgttggcga
     5941 tgaagatgcg gctgacgaag tcctcatcgc gcgtcgccat gccggcacgg cccttgccgc
     6001 cgcgccgctg cgcgcgatag gccgaaagcg ggacgcgctt gatgtaaccc ttgtgggaca
     6061 cggtcacgac catgtcctcg cgctggatca ggtcctcgtc ctcgacctcg cccaccatgt
     6121 cgacgatttc ggtacggcgt ggcgtgccga actgcttttt cagttcggtc aactcgtcct
     6181 tgacgatgcc gacgatccgc gcgcgcgacc gcaatatttc caggtaatcg gc
//
LOCUS       DBNHVU010000168         6336 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_804152, whole genome shotgun sequence.
ACCESSION   DBNHVU010000168 DBNHVU010000000
VERSION     DBNHVU010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6336)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6336)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6336
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_804152"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /gene="mepA"
                     /locus_tag="AC16Y7_06875"
     CDS             complement(<1..999)
                     /gene="mepA"
                     /locus_tag="AC16Y7_06875"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HIR5709140.1"
                     /translation="MRRLILSLTILLLGWGLATAQEKLAPPAGGTAEAESGEAAETEA
                     GEPTETAAIEPAMELPERKPPKPPEPKSKKLPGARELFASVPGPAKLAARAIGFYSKG
                     CLSGGIALPLNGPDWQVMRVSRNRYWGSPQLVDYMERLASDARALDGWPGLLVGDMSQ
                     PRGGPMLTGHTSHQVGLDADIWLTPMPDRILTLQEREDVEAVSMLKDPLHVDPEKFTD
                     VHIKLIRRAASYRQVARIFVHPAIKRSLCERAEVAGKDTRWLGKVRPWWNHHYHFHVR
                     LGCPRGMAGCKDQPPVKGDSGCGKELDDWNSMLKKSSIWRTQQNIDPNAKPGIKKKP"
     gene            1216..1641
                     /locus_tag="AC16Y7_06880"
     CDS             1216..1641
                     /locus_tag="AC16Y7_06880"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR5709141.1"
                     /translation="MKRNVVLAALLGTFLVLPATAFAGVVAQIDLSSQRMHVFVNGKS
                     TYTWKVSTARRGYRTPTGTYKPYHLARRHYSSIYNNSPMPHSIFFKGGYAIHGSYETK
                     HLGRPVSHGCIRLHPSNAARLFSLVQKYGNSNTTIRITH"
     gene            1920..3059
                     /locus_tag="AC16Y7_06885"
     CDS             1920..3059
                     /locus_tag="AC16Y7_06885"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5709142.1"
                     /translation="MPSAASRLDAFIDRVSGIATPFSIELPDGAKRTVGAGQPEFYVD
                     LHNERAIKALRTLDEGDIAEAYLQGDIDIEGDMLKPFALRASLDDSHPLVAAWRFIQP
                     ALFGQVYTNKQAIASHYNLDPKLFLSFLDPVFPAYSQGIYESDDEPLSKALERKFDWA
                     IEKCELAPGKRVLEIGPGWGAFPSHALQSGIKYTGITNSEVSQNFLRDKLANFGDQFE
                     ILLTDFYDYEPNEPFDAIVIMGVIEHLPNYEKVLKKFFRLLKPGGLVFLDGSAARTKY
                     ELSTFMVRYIYAGNHSFLVLDDFLNKMNKTEFEAEEIHSDRWSYFLTFKQWAENLESK
                     KDYVLKTFGDFEYRRFRLYLWGAAYEFMARNLDCYRMILSKPKDA"
     gene            3134..3853
                     /locus_tag="AC16Y7_06890"
     CDS             3134..3853
                     /locus_tag="AC16Y7_06890"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HIR5709143.1"
                     /translation="MSGDHKTPSLSRRGLMAGVGALFAASTMRPVVANAADDKAPAPN
                     TIPPSEALGRLKAGNARYVANEPKATDFSPGRAARTKVQHPIAGILSCADSRVPPEII
                     FDQGPGDLFVSRDAGNVVSNYGLASFEFAVANLGVPLILVLGHTGCGAVAAALGASTS
                     RKELPGHLPELVKAIEPAIITAHGKHPGDFLAASIEENVRLGMKRLKTKSEIIGKAVN
                     SGKLQIKGGIYDLKTGSVTFV"
     gene            4001..4936
                     /locus_tag="AC16Y7_06895"
     CDS             4001..4936
                     /locus_tag="AC16Y7_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709144.1"
                     /translation="MSHQILLHSIGSLRALGCAVAIALAALVFTPPSVLGQDAPAGEA
                     ATEDAATEDAAAEETTDDTKTDDDAAEEKKPWNVAAPCDNVQHPYEADLCQQWRGAEA
                     AESTAGLTRLLTWIGAIATLLLMLMFVPLVMALQAARRTSDVSAPMPAPVPAGSGGAS
                     EEELRAYVDVDKLEFIETPESEGVVKVKVVYRNSGQTPAFRIKSAAEVGIRDVSDEDL
                     LPIMQLPQRSSLDSPRPRLGRDATDTEIVTCESTASLADRVMGGDATILVWGVVEYLD
                     VFDRPQKTAFQYLCNAETLDSGQIFKPMVRGDEDG"
     gene            complement(4946..6241)
                     /locus_tag="AC16Y7_06900"
     CDS             complement(4946..6241)
                     /locus_tag="AC16Y7_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5709145.1"
                     /translation="MDHEQTSDGYLSAAIAKATYRLIPFLCLAYTVNFLDRVNVGFAA
                     LHMNADLGFSPAVFGFGAGIFFLGYIVFEIPSNLALQRFGARIWIARIMISWGLIACA
                     MALVRGETSFYVSRLLLGIAEAGFFPGIILYLTYWFPAGTRARIIALFMASVPLATVF
                     GGPISGVLLQMHGFAGLAGWQWLFIIEGAPAVLLGVLALFVLTDKPDKAAWLSENERR
                     ALTETLASEAANTKAVGYADLRQALKRPRVLALGVLYFLMVTGLYGIGFWMPQVLATF
                     GLDPLHVGFLTAIPYLFAVIAMVLWGRHSDKTGERRWHIALPLLLAACAFAWSAYSGP
                     LLPTMIALSLATLGFYAAFGPFWSLPTALLTGTGAAAGIALVNSMGNAAGFTGPYIVG
                     LLKQATGSFSAALLFLAAALALGGLMALCFKRIEDPRHT"
BASE COUNT         1243 a         1983 c         1982 g         1128 t
ORIGIN      
        1 aggcttcttc tttattcccg gcttggcgtt tggatcgatg ttttgctgcg tccgccagat
       61 cgaggacttc ttcagcatag agttccaatc gtccagctcc ttgccgcagc cggaatcgcc
      121 cttcaccggc ggctgatcct tgcagcctgc catgccgcgt gggcagccga ggcggacgtg
      181 gaaatggtag tggtggttcc accacggccg caccttgcca agccaacgtg tgtctttgcc
      241 tgcgacctcg gcgcgctcgc atagcgaccg cttgatcgcc gggtgcacga agatgcgcgc
      301 cacttgtcta taagacgcgg cgcgacggat gagcttgata tgcacgtcgg tgaacttctc
      361 cggatcgacg tgcaacggat ccttcagcat cgaaaccgct tcgacgtcct cccgctcttg
      421 gagcgtaagg atgcggtccg gcatgggcgt tagccagatg tcggcatcga gcccaacttg
      481 gtgactggta tggccggtga gcatcggccc gccgcgcggc tgtgacatgt cgccgacgag
      541 aaggcccggc cagccgtcga gggcgcgcgc gtcgctggca agccgctcca tatagtcgac
      601 gagctgcggg ctgccccagt agcggttccg cgagacgcgc atcacctgcc agtcggggcc
      661 gttgagggga agcgcgatac cgcccgaaag gcagcccttc gaataaaagc caattgcccg
      721 tgccgcgagc ttggcagggc cggggacgga ggcgaacagc tctctggcgc ccggcagttt
      781 tttcgacttc ggctctgggg gcttgggcgg cttgcgttcc gggagttcca tggcgggttc
      841 gatggccgcc gtctcggtag gctcgccggc ctctgtctcc gcagcttcgc cggattccgc
      901 ctcagcggtg ccgccagccg gcggcgcgag cttctcttgc gccgtcgcca aaccccagcc
      961 aagcagcaat atcgtgaggc ttagaatcag ccttcgcatg cgttcccgac ctggttttga
     1021 caggcccccc ggaggcgcac agtagcacaa atggtgtcgg caattggacc cctttgcggt
     1081 gcgccgaagc cacaccgttc aggagatgtt cagccccgcg cgccaatagt ctgatcaatg
     1141 tcgttggaat ctagagcgtc aggcgcgatg atgtgcgggc gcgggacaca aattccaagt
     1201 ctgtgggagc tgatcatgaa acgtaacgtc gtcttggccg cgctgctcgg cacatttctt
     1261 gtcctgcccg cgaccgcctt cgccggtgtc gtcgctcaaa tcgatctctc cagccagcgc
     1321 atgcatgtgt tcgtgaatgg caagtcgacc tacacgtgga aggtctccac ggcgcgacgc
     1381 ggctaccgca cgccaaccgg cacctacaag ccgtatcatc tcgcccgccg ccactactcc
     1441 tcgatctaca acaactcgcc catgccgcat tcgatcttct tcaagggcgg ttacgccatt
     1501 cacggctcct acgagacgaa gcatctcggg cgcccggtct cccatggctg cattcggctg
     1561 catccctcaa acgcggcgcg gctgttctcg ctggtgcaga aatacggcaa cagcaacacg
     1621 acgatccgca tcacccacta acgcgagaag actaacggcc gcagacgctt ggcgcggggc
     1681 gcccgcgaac gcggtcggcg gcgagccgaa gccaggacgt gaggattgac atttgggaaa
     1741 ccctctaata cttgcgcgcg gcggatcata gcccgagaag cggcggtgtc cgccttttga
     1801 aattgagctg ggccgggctt ttcatgacgc ccgcttctag ggcacaatcg gacagagcaa
     1861 gaggctgcct gcctgcgcgg gccaccaacg cgggtcttat gagtcgggga acggttcgga
     1921 tgccatcagc cgctagcagg ctcgacgcct tcatcgaccg tgtcagcggg attgcgactc
     1981 ctttctccat cgaactcccc gatggcgcca agcgcaccgt gggcgcgggc cagcccgagt
     2041 tttacgtcga tctccacaac gagcgggcga tcaaagcctt gcggaccttg gacgagggcg
     2101 atatcgcaga agcctatttg cagggcgaca tcgacatcga aggcgacatg ctgaagcctt
     2161 ttgcgctgcg ggcctcgctc gacgacagtc acccgctggt cgccgcgtgg cgcttcatcc
     2221 agcccgcgct gttcgggcag gtctacacca acaagcaggc gatcgcttcg cactacaatc
     2281 tcgacccgaa gctgttctta agcttcctcg accccgtgtt cccggcctac agccaaggca
     2341 tctacgaaag cgacgacgag ccgctgtcga aggcgctgga gcggaagttc gactgggcca
     2401 tcgagaagtg cgaactggcc cccggcaagc gcgtgctgga aattggaccg ggctggggtg
     2461 ccttccccag ccacgccctg caatccggca tcaaatatac cggcatcaca aactcggaag
     2521 tctcgcagaa cttcttgcgc gacaagctcg ccaatttcgg cgaccagttc gagatcctgc
     2581 tcaccgactt ctatgattac gaaccaaacg agccgttcga cgccatcgtt ataatgggcg
     2641 tcatcgagca tctgccgaac tacgaaaagg tgctgaagaa attcttccgg ctcttgaagc
     2701 ccggcggcct cgtgttcctc gacggcagcg cggcgcggac gaagtatgag ctgtcgacct
     2761 tcatggtccg ctatatttat gcgggcaacc actccttcct cgtgctcgac gactttctca
     2821 acaagatgaa caagaccgag tttgaggcgg aggaaatcca ctcagaccgc tggagctact
     2881 tcctcacctt caagcagtgg gccgagaact tggagtccaa aaaggactac gtgctgaaga
     2941 cgttcggtga tttcgaatac cgccgcttcc gtctctatct ttggggtgcg gcttacgagt
     3001 ttatggcgcg caatctcgat tgctaccgta tgatcctctc caagccgaaa gacgcctgac
     3061 gcaggcttga ccgcacctgt gcctcggggg aggctcggcg gaggggtatc actctgccgg
     3121 gagggcacaa agcatgtccg gagaccacaa gacaccaagt ctgtcacgcc gcgggctgat
     3181 ggccggcgtc ggggccttgt ttgccgcaag cacgatgcgg cccgtagtcg ctaacgccgc
     3241 cgatgacaaa gcgccggcgc cgaacaccat tccgccatcg gaagccctcg gaaggctgaa
     3301 ggctggcaac gcccgctatg tggcgaacga gccaaaggcg acggatttct cgcccggccg
     3361 cgccgcgcgc acgaaagtac agcatcccat cgctggcatc ttgtcctgtg cagacagccg
     3421 cgtccccccg gagattattt tcgatcaggg ccctggtgat cttttcgtat cgcgcgatgc
     3481 cggtaacgtc gtcagcaatt atggcctggc gagcttcgag ttcgccgtgg ccaatctcgg
     3541 cgttccgcta atccttgtgc tcggtcacac tggctgcggc gcggtcgcgg cggcgcttgg
     3601 ggcctccacg tcacgaaaag agctgcccgg tcatctgccg gaactcgtca aggcgatcga
     3661 gccggcgatc atcaccgccc atggcaagca ccctggcgat tttctcgcag caagcatcga
     3721 ggagaatgtg cgcctcggca tgaagcggct gaagacgaag tcggagatca ttgggaaggc
     3781 tgtcaattcc ggtaagttgc agatcaaggg cggcatctac gaccttaaga ctggctccgt
     3841 gacattcgtc tagtcgaagg gctcgcggcg cgcctccaaa attgtcccat tttcgaacgg
     3901 attggcaaga agtttgcgac aagcgagcaa gaaggcgaga caaccggcct ttccatgaat
     3961 aggatcggcg gcctacgggg aaattgtctg cgcctttgct atgtctcatc aaatcctgct
     4021 ccattccatt ggaagcctgc gcgcgctcgg ctgcgccgtt gccatagcgc ttgccgcgct
     4081 ggtcttcacg ccgccgtctg ttctcggaca agacgcaccg gccggagagg ctgcgaccga
     4141 agacgctgcg acagaagacg ccgcggctga ggaaactacc gacgacacca agacagacga
     4201 cgacgcggcg gaagagaaga agccgtggaa cgtcgcggcg ccttgcgaca acgtccagca
     4261 tccttacgaa gcggatctct gccagcaatg gcgcggggct gaagcagccg agagcaccgc
     4321 cgggctgacg cgcctgctga cgtggattgg cgcgatcgcg acattgctgc tgatgctgat
     4381 gttcgtgccg ctcgttatgg cgttgcaggc ggcgcgacgg acgtcggacg tcagtgcgcc
     4441 catgcctgcg cccgtccccg ccggcagtgg cggcgcgagc gaggaagagc tgcgcgccta
     4501 tgtggatgtc gacaagctcg agttcatcga gacgccggag tccgagggcg tcgtcaaggt
     4561 gaaggtggtg taccgcaatt ccggtcagac gcccgcgttc aggatcaaga gcgcggcgga
     4621 ggtcggcatt cgcgacgtgt cggatgagga tttgctgccc atcatgcagt tgccgcagcg
     4681 ttcgtcgctg gactcgccaa ggccgcgtct tggccgcgat gcaacggaca cggagatcgt
     4741 cacttgcgag tccacggcga gccttgccga ccgtgtcatg ggcggcgacg cgacgatcct
     4801 agtttggggc gtcgtggagt atttggacgt cttcgaccgc ccgcagaaga ccgcgttcca
     4861 gtatctgtgc aacgccgaga ctctcgacag cggtcagatc ttcaagccca tggtgcgcgg
     4921 cgacgaggac ggttaggacg cacgcctaag tgtggcgcgg atcctcgatc cgcttgaagc
     4981 acagcgccat caggccgccc agcgccagcg cggccgccaa gaacagcagc gccgccgaga
     5041 agcttcccgt cgcttgtttt aagagcccga cgatatatgg gcccgtgaag cccgccgcgt
     5101 tccccatgga attcaccagc gcgatgcccg ccgcagcgcc tgtccccgtc agcagcgccg
     5161 tgggcagcga ccagaacggc ccgaacgcgg cgtagaagcc gagcgttgcc aagctcagcg
     5221 cgatcatggt ggggaggagc ggccccgaat aggcggacca ggcgaaagcg cacgccgcaa
     5281 gaagcagcgg aagcgcaata tgccaacggc gctcgccggt cttgtcggaa tgccgccccc
     5341 acagcaccat ggcgatgacc gcaaacaaat atgggatcgc ggttagaaag ccgacatgca
     5401 gcggatcgag gccgaaggtt gcgagcactt gcggcatcca aaagccgata ccgtagagcc
     5461 cggtcaccat gagaaagtag aggacaccga gcgccagcac gcgcgggcgc ttcagcgctt
     5521 ggcgaagatc cgcatagccg accgctttcg tgttcgccgc ttcgcttgca agcgtctcgg
     5581 tgagcgcgcg ccgctcgttc tcgctcagcc acgccgcctt gtccggcttg tcggtgagga
     5641 cgaacagcgc cagcacaccg agaagaaccg cgggcgcgcc ttcgatgatg aacagccact
     5701 gccagcccgc gagtcccgcg aagccgtgca tctgcaaaag cacgccggag atcgggccgc
     5761 cgaacaccgt ggcgagcggc actgatgcca tgaacagcgc gatgatgcgg gcgcgtgtcc
     5821 ctgccgggaa ccagtaggtg agatagagga tgatgccggg gaagaagccg gcttcggcga
     5881 tgccgagcag aagccgcgag acgtagaagc tcgtctcgcc gcgcaccagc gccatggcgc
     5941 aagcgatcaa gccccaggag atcatgatgc gcgcgatcca gatgcgcgcg ccgaagcgct
     6001 gcaacgcgag attgctgggg atctcgaaaa cgatgtagcc gagaaagaag atcccggcgc
     6061 caaagccgaa cacggcgggc gagaagccga ggtccgcgtt catgtgcagc gcggcgaagc
     6121 cgacattcac ccggtcgaga aaattgaccg tgtaggcgag gcataggaac gggatcagcc
     6181 ggtaggtggc cttggcaatt gcggcggaga ggtacccgtc tgaagtctgc tcgtggtcca
     6241 tgtgcccccg tgaatcgcgt atgcttggca cgcggccgcg cgattgattc ggccgccaac
     6301 ctagaggagg gctctaccca tggctattcc ggtcga
//
LOCUS       DBNHVU010000169         7457 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1547189, whole genome shotgun sequence.
ACCESSION   DBNHVU010000169 DBNHVU010000000
VERSION     DBNHVU010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1547189"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..955
                     /locus_tag="AC16Y7_06905"
     CDS             <1..955
                     /locus_tag="AC16Y7_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5709146.1"
                     /translation="CKFIAFMPQWHFLDFLALKAKQYPGFHLRMQAEVDELIKDGDRV
                     AGLKAASPQGDLEVHADLVVGTDGRHSTVRNLAGLEVENRGAPMDVLWLRLSKRPEDG
                     SESLGHIEPGRFFITIDRGDYWQCAFVIPKGGFNELRRKGLEAFRLAIVALQPSLSNR
                     VREIVSWDDVKLLTVRVDRLKRWYRPGLLCIGDAAHAMSPIGGVGINLAIQDAVAAAN
                     ILAGPLREGKVTEDDLRKVEKRRQLPTELTQGLQIFLQNRVISNVLTMNKRPKPPFMV
                     KMFNRFPVLRRLPARLIGMGFRPEHVKTQEAPAQAAKPQSS"
     gene            complement(952..1944)
                     /locus_tag="AC16Y7_06910"
     CDS             complement(952..1944)
                     /locus_tag="AC16Y7_06910"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR5709147.1"
                     /translation="MASDQPLVIVTRKLPDVVETRLKELFDARLNIEDKPFSRDDLID
                     AVKTADVLVPTVTDRIDRAVLSQAGPNLKLIAQFGTGVDNIDLDTARNRSILVTNTPG
                     VLTEDTADMTMALILAVPRRLTEGAAMLKAGSDKWQGWSPTWMLGHRIFGKRLGIVGM
                     GRIGQAVAKRAKAFGLQIHYHNRRRVQPDVEQALEATYWDSLDQMLARMDIISINCPH
                     TPATYHLLSARRLSLLKPSAYIVNTARGEVIDENALARMIDNNELAGAGLDVFEHEPA
                     VNPKLLRSDKVVVLPHMSSATLEGRVDMGEKVIINIKTFLDGHAPPDRVHPAMF"
     gene            2153..2686
                     /locus_tag="AC16Y7_06915"
     CDS             2153..2686
                     /locus_tag="AC16Y7_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR5709148.1"
                     /translation="MRTGRLIAIGLALALVPWAGSAPAQEGAKQAPNGSQSGLRVPRF
                     VSLKSNKVNVRRGPSTDQAIVWVFARAGLPVEIIAEADNWRRVRDSEGADGWVFHSLL
                     SGRRTALISPWAKGQAVPLRDGASAGSRPVAQLQSGVLGNVVSCDGKWCELTVDGYSG
                     YIEQEKLWGVYRGEEID"
     gene            complement(2683..3090)
                     /gene="irrA"
                     /locus_tag="AC16Y7_06920"
     CDS             complement(2683..3090)
                     /gene="irrA"
                     /locus_tag="AC16Y7_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron response transcriptional regulator IrrA"
                     /protein_id="HIR5709149.1"
                     /translation="MTGSVAEILRGAGLRPTRQRVALARLLFAEGDRHVTAERLHEEA
                     LASKVPVSLATVYNTLHQFTEAGLLREVAVEGAKTYFDTNTSNHYHFFCERSGRLLDI
                     DTADIRIEGLPEAPEGMAVSRVDVLVRLVNKNS"
     gene            3374..3922
                     /gene="fabA"
                     /locus_tag="AC16Y7_06925"
     CDS             3374..3922
                     /gene="fabA"
                     /locus_tag="AC16Y7_06925"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007065727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     FabA"
                     /protein_id="HIR5709150.1"
                     /translation="MSVQKSTADRQASFDYEALLACARGELFGPGNAQLPLPPMLMFD
                     RISHVEEQGGEHGRGRIVAELDVTPDLWFFACHFKGDPVMPGCLGLDALWQMLGFFLG
                     WVGAPGKGRALGVGEVKFSGMVLPTMKKLEYIVELRRVIRRKFTLGTGDGWLKADGET
                     IYTAKDLKVGLFQADDAASAEA"
     gene            3956..5176
                     /gene="fabB"
                     /locus_tag="AC16Y7_06930"
     CDS             3956..5176
                     /gene="fabB"
                     /locus_tag="AC16Y7_06930"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009796638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HIR5709151.1"
                     /translation="MKRVVVTGMGIVSSIGNSAQEVLASLREAKSGIVKADTYAELGF
                     RCQVHGAPNIDWEGMIDRKVRRFMGAGAGWNYIAMEQAIRDAGLEESDVSNERTGLIM
                     GSGGPSTRAIVKAADTTRDRGPKKVGPFEVPKSMCSTNSATLSTPYHIKGVSYSISSA
                     CSTSAHCIGNAGELIQLGKQDIVFAGGGEELDWTLSVLFDSMNAMSSDFNDQPDKASR
                     AYDTARDGFVIAGGAGVLVLEELEHAKARGAKIYGELVGYGATSDGFDMVLPSGEGAV
                     RCMRMAMQNLNGDKIDYINPHATSTPQGDVREIEAIREVFGDDRPPISATKSLTGHSL
                     GAAGVHEAIYSLLMMNNGFICESANIENLDPDLADMPIVRKRKDGAALDTVMSNSFGF
                     GGTNASLVFRRLDS"
     gene            5226..6110
                     /gene="fabI"
                     /locus_tag="AC16Y7_06935"
     CDS             5226..6110
                     /gene="fabI"
                     /locus_tag="AC16Y7_06935"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIR5709152.1"
                     /translation="MATKNDIAEPGPLMAGKRGLVMGVANDRSIAWGIARVLHGQGAE
                     LAFSYQAGAFGRRAAPLAQSIGAKIIEEVDVEDLDSVDRMLDVIRKEWGSLDFVVHAL
                     AFSDRNELKGRYCDTTRANFEHTMVISCFSFTEICKRASDMMSDGGSLLTLTYGGSTR
                     VVPCYNVMGVAKAALETSVKYLASDLGPRGIRVNALSAGPMRTLAGAGISDARTLFHY
                     QQKHAPMRRSPTLDEVGGAALYLLSDMSGAVTGEVHFVDCGYSTTFMPDLESLKSIEQ
                     AEEFESAMQKATPREAAQ"
     gene            6269..6481
                     /locus_tag="AC16Y7_06940"
     CDS             6269..6481
                     /locus_tag="AC16Y7_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709153.1"
                     /translation="MFQVILMVCLAATGEQCKEFKLTKEYTDAVSCIRDSHGVGSVWQ
                     GENTKYTIIGTRCTKDTTKIPKAPNS"
     gene            complement(6549..>7457)
                     /locus_tag="AC16Y7_06945"
     CDS             complement(6549..>7457)
                     /locus_tag="AC16Y7_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR5709154.1"
                     /translation="KLAKRGIQAVMPDLDDFGYVIRVVSEITESNGSSSMASVCGASL
                     SLMDAGVPLKAPCAGIAMGLIKEGDKFAVLSDILGDEDHLGDMDFKVAGTSEGVTSLQ
                     MDIKITGITEEIMKIALDQAKGGRMHILGEMDKALTGARPELGEYAPRIETINIPVDK
                     IREVIGSGGSVIREIVAESGAKIDLSDDGTIKIASSNAESIRDAIARIKSIAAEPEVG
                     VIYQGKVVKVMEFGAFVNFFGPRDGLVHISQLDEGRPKQVTDVVKEGDQVWVKLLGFD
                     ERGKVRLSMKVVDQDTGKEKEPAEAS"
BASE COUNT         1380 a         2269 c         2403 g         1405 t
ORIGIN      
        1 ctgcaagttc atcgccttca tgccgcaatg gcacttcctc gattttctcg ccctgaaggc
       61 aaagcaatat cccggctttc atttgcgtat gcaggcggaa gtggacgagc tgatcaagga
      121 cggcgatcgc gtggccggcc tgaaggcggc gtccccgcaa ggcgacctgg aggttcacgc
      181 cgatctcgtg gttggcactg acgggcggca ctcgacggtc cgcaatctcg cgggacttga
      241 ggtcgaaaat cgcggcgcgc ccatggacgt cttgtggttg cgtctatcca agcgcccgga
      301 ggacggctcg gagagcctcg gccatatcga gccgggacgc ttcttcatca cgatcgaccg
      361 cggcgactac tggcagtgtg ccttcgtcat tccgaaaggc ggtttcaacg agttgcgccg
      421 caaagggctg gaagcgtttc gtctggcgat cgtcgcgctc cagccgtccc tgtcgaaccg
      481 cgtgcgcgag atcgtgtcct gggatgacgt caagctgctg accgtgcgcg tcgaccgctt
      541 gaagcgctgg taccggccgg gtctcctgtg cattggcgat gcggcgcacg ccatgtcacc
      601 gatcggaggc gttggtataa acctggcgat tcaagatgcc gtcgcggcgg ccaatatcct
      661 ggcgggcccg ttgcgcgaag gcaaagtcac cgaagacgat ttgcggaaag tggagaagcg
      721 gcggcaattg ccgacggaac tgacgcaagg tctgcagatc ttcctccaga accgcgtcat
      781 ctccaacgtg ctcacgatga acaagcggcc gaaaccgccc ttcatggtca agatgttcaa
      841 tcgcttcccg gttctcaggc ggcttccggc gcgtctgatc ggcatgggct tccggcccga
      901 gcacgtgaaa acgcaagaag cgccggcgca agcggccaag ccgcaaagct cttagaacat
      961 tgcggggtgg acgcggtccg gcggcgcatg gccgtcgagg aaggtcttga tgttgatgat
     1021 gaccttctcg cccatgtcga cccggccctc gagcgtcgcc gagctcatat gcggcaggac
     1081 cacgactttg tcgctgcgca ggagcttcgg gttcaccgca ggctcgtgct cgaacacatc
     1141 gagcccggca ccggcaagtt cgttgttatc aatcatgcgg gccagagcat tctcgtcgat
     1201 cacctcgccg cgcgccgtgt tgacgatata ggcgcttggc ttgagcagcg acagccgccg
     1261 cgccgacagc aaatgatagg tcgccggcgt gtgcgggcag ttgatggaga taatgtccat
     1321 gcgcgccagc atctggtcga ggctgtccca ataggtcgcc tcgagcgcct gctcgacatc
     1381 gggctgaacc cggcgccggt tgtgatagtg gatctgaagg ccgaaggcct tggcgcgctt
     1441 ggccacggct tggccgatgc gccccatgcc gacaatgccg agcctcttgc cgaagatgcg
     1501 gtggcccagc atccaggtgg gcgaccagcc ctgccatttg tcgctgccgg ccttgagcat
     1561 ggccgcgcct tccgttaggc gccgcggcac ggccagaatg agcgccatgg tcatgtcggc
     1621 cgtgtcctcg gtcaacacgc ccggcgtgtt ggtgaccagg atggagcggt tccgcgccgt
     1681 gtcgaggtct atattgtcga cgccggtccc gaactgcgcg attagcttga ggtttggccc
     1741 ggcctgactc agcacggccc ggtcgatgcg gtcggtgacc gtgggcacga ggacgtcggc
     1801 ggtcttgacc gcgtcgatca ggtcgtcccg cgagaacggc ttgtcttcaa tgttgagccg
     1861 cgcgtcgaac aactccttaa gccgtgtctc cacgacatca ggcagcttac gcgtgacaat
     1921 aacgagcggt tggtcagaag ccatgatcta tcccgtcagg tggcccgcgg tgggtaggtc
     1981 ccacggtgga cgccacatgc ttgatttgcg cgctttgtct aacagaaggc ggattaagag
     2041 ataaagcgtc atattggctc tgggggcgga aatgcacccg ggccacgatc cgttcacttt
     2101 tctcggccat tcaaggggca tcgattcggg ggacggaaga ggggaatcgc tggtgcgcac
     2161 tggcaggttg atcgcgattg gactggctct cgccctggtg ccgtgggcgg ggagcgcgcc
     2221 ggcccaggaa ggcgcaaagc aggcgcccaa cggctcgcag tccggcttgc gcgtaccccg
     2281 tttcgtcagc ctcaagtcca acaaggtcaa tgtccgccgg ggccccagca ccgaccaggc
     2341 gatcgtctgg gtcttcgccc gcgccggcct gccggtggag atcatcgccg aggccgacaa
     2401 ttggcgccgg gtccgtgaca gcgaaggcgc cgatggctgg gtgttccata gcctcttaag
     2461 cgggcgacgg accgcgctca tcagcccctg ggccaagggc caggccgtgc cgctgcgcga
     2521 tggggcgtcg gcagggtcgc ggcccgtggc ccagcttcaa tccggcgtgc tcggcaacgt
     2581 ggtctcgtgc gatggaaaat ggtgcgaact cacggtcgac ggctattccg gctatatcga
     2641 gcaggaaaag ctgtgggggg tctaccgggg cgaggagatc gactagctgt tcttgttgac
     2701 cagccgcacc aggacatcga cacgcgaaac ggccatgccc tcaggtgcct caggcaagcc
     2761 ctcgatgcgg atatcggccg tgtcgatgtc gagaagccga cccgaacgct cgcagaagaa
     2821 gtgatagtgg ttcgacgtat tggtgtcgaa ataggtcttg gcgccttcaa cggccacctc
     2881 acgcagaagc cccgcctcgg tgaactggtg cagcgtgttg tacacggtcg ccagcgacac
     2941 gggcaccttg gacgccaaag cctcctcgtg cagccgctcg gccgtgacat gccggtcgcc
     3001 ctcggcgaac agcagccgcg ccagggctac ccgctggcgg gtaggccgca agcctgcacc
     3061 cctcaggatt tcggcaacac tgcctgtcat gatttcggcg tccttctgag gtgcctgcga
     3121 gggattgtcc atggtcttcg tttcgcgcga tgatcgatga taggaaatat aggcggtcac
     3181 ggtctccccc gcaacgctgt gggatggttg actttttgcc gcggtatccc ggcccgaagg
     3241 ggtgatcgga gaggtgaaat ggcccggcgc gccagggcca tggcggccct aaagagccaa
     3301 cggccgggct ttttgtgggt cccggcatat gttaactaac cagcccatat ctcaggccta
     3361 aagcgaagga cgaatgagcg tgcagaaatc gacggccgac cggcaggcaa gtttcgacta
     3421 tgaggctttg ctggcttgcg ccaggggcga gctattcggc cccggtaacg cccagcttcc
     3481 cttgccgcca atgctgatgt tcgaccgcat ttcccatgtc gaggagcagg ggggcgagca
     3541 cggccgtggc cgcatcgtgg ccgaactcga cgtgacgccc gatctctggt ttttcgcctg
     3601 ccacttcaaa ggcgaccccg tgatgccggg ctgcctcgga ctcgacgccc tatggcagat
     3661 gctcggtttc ttcctcggct gggtcggggc tcccggcaag ggccgcgcgc tcggggtcgg
     3721 cgaggtcaag ttctccggca tggtgctgcc caccatgaaa aagctcgaat atattgtcga
     3781 actgaggcgc gtcattcggc gcaagttcac gctcggcacc ggcgatggct ggctcaaggc
     3841 cgacggcgaa acgatttaca cggccaagga cctcaaggtc ggactattcc aggcggacga
     3901 cgctgcttcc gccgaagctt gatttagcgg catagatttc aaggacgctg aaagtatgaa
     3961 acgcgtggtc gtcaccggca tgggcatcgt gtcctccatc ggcaacagtg cccaggaggt
     4021 gctcgcttca ttgcgggagg ccaaatccgg aatcgtcaag gccgacacct atgcggagct
     4081 ggggttccgc tgtcaggtgc acggggcgcc gaatatcgac tgggaaggga tgatcgaccg
     4141 caaggtccgc cgcttcatgg gcgctggcgc aggctggaac tatatcgcca tggagcaggc
     4201 gatccgcgat gcgggccttg aggagagcga tgtctccaac gagcgcaccg ggctgatcat
     4261 ggggtccggc ggcccctcga cccgcgccat cgtgaaggcg gccgatacca cccgcgatag
     4321 gggccccaag aaggtcggcc ccttcgaggt gccgaaatcc atgtgctcca ccaattctgc
     4381 gacgctctcc acgccgtatc acatcaaggg cgtcagctat tcgatttcgt cggcctgttc
     4441 caccagcgcc cactgcatcg gtaacgcggg cgaactgatc cagctcggca agcaggacat
     4501 tgtgttcgcc ggcggtggcg aggaattgga ctggaccttg tctgtgctgt tcgattccat
     4561 gaacgcgatg tcgtcggact tcaacgatca gcctgacaag gcgagccgcg cctacgacac
     4621 ggcgagggat ggtttcgtca tcgcgggcgg agccggcgtt cttgtgcttg aggaactgga
     4681 gcacgccaag gcgcgcggcg cgaagatcta cggtgagctc gtcggctacg gtgcgacgtc
     4741 ggacgggttc gatatggtgc tgccttccgg agagggcgcg gtgcgctgca tgcgcatggc
     4801 gatgcagaac ctcaatggcg acaagatcga ctacatcaac ccgcacgcca cttcgacgcc
     4861 gcagggcgat gtgcgggaga tcgaagcaat ccgcgaggta ttcggcgacg accgtccgcc
     4921 catcagtgcc accaagtcgc tgaccggaca ttcgctcggc gccgccggtg tgcacgaggc
     4981 gatctactcg ctcctgatga tgaacaacgg tttcatttgc gagtccgcaa atattgaaaa
     5041 cttggatccg gacttggccg acatgccgat cgttcgcaaa cggaaggacg gcgcggccct
     5101 cgacaccgtg atgtcgaata gttttggttt tggcggtacc aatgcatcac tcgtcttccg
     5161 ccgattggat agctaaaagc cggtgaacgg cagaattaaa gtcaactcga agcagagtaa
     5221 gtaacatggc aacaaagaac gacattgctg agccggggcc gctgatggct gggaagcggg
     5281 gcctcgttat gggtgtggcc aacgaccggt caatcgcctg gggcattgcc cgggtcctgc
     5341 atggtcaggg cgcggagctg gcattcagct atcaggcggg tgctttcggg cgccgtgccg
     5401 cgcctcttgc gcaatccatc ggcgccaaga tcatcgaaga ggtcgatgtt gaggatctgg
     5461 acagcgtcga ccgcatgctg gacgtgatcc gcaaagagtg gggttccctc gatttcgtcg
     5521 tgcacgcgct cgccttctcc gatcgcaacg agttgaaggg ccgctactgc gacacgacac
     5581 gtgcgaactt cgagcacacc atggtgatct cctgcttttc cttcacggag atctgcaagc
     5641 gcgcctccga catgatgagc gatggcggtt cgctgctcac gctcacctat ggcggttcga
     5701 cccgcgtggt gccttgttac aatgtgatgg gtgtggccaa ggccgcgctg gaaaccagcg
     5761 tgaaatacct cgcctcggat ctgggtccgc ggggcattcg cgtgaacgca ctttcggccg
     5821 ggcccatgcg gacgctggcc ggcgccggca tttccgatgc gcgcacgctg ttccactatc
     5881 agcaaaagca cgcgcccatg cgccgctcgc ccacgctgga cgaggtcggc ggggccgcgc
     5941 tctatctctt gtccgatatg tcgggcgccg tgaccggcga ggtgcatttc gtggactgcg
     6001 gctactcgac caccttcatg cccgatctgg agtcgttgaa gtcgatcgag caggccgagg
     6061 agttcgaaag cgcgatgcag aaggccacgc cccgcgaggc cgcccaatag aggcccgcga
     6121 ggccgcccag caggcggtct gaaagcgggt aacgctgtgt gcgtggccat attgacatgg
     6181 gcccgcacgg tcacgatttt accgtctaga caggtacata gggcccgccg cgtgccgagg
     6241 cgaagatgtg gtccgccaag gaggaaacat gttccaagtg attttgatgg tgtgtcttgc
     6301 cgctaccggc gagcagtgca aggagttcaa gctcaccaag gagtacaccg acgccgtgag
     6361 ctgcatccgc gacagtcatg gcgtgggcag cgtgtggcag ggcgagaaca ccaagtacac
     6421 gatcatcggt acgcggtgca ccaaggacac aaccaagatc cccaaggcgc cgaacagcta
     6481 gccacaaacg gatcggggcg ccgaagcgcc cctctcaata ttctgacgat tggtaaggtt
     6541 ccgccggcct agctggcctc ggcgggttcc ttttccttgc cggtatcctg gtcgacgacc
     6601 ttcatcgaca ggcggacttt gccgcgctcg tcgaagccga gcagcttgac ccagacctgg
     6661 tcgccttcct tgaccacgtc ggtgacctgc ttcgggcggc cctcatccaa ctgcgagata
     6721 tggaccaagc cgtcgcgcgg gccgaagaag ttcacgaacg cgccgaactc catcaccttc
     6781 acgaccttgc cctggtagat cacgccaacc tccggctctg ccgcgatcga cttgatgcgg
     6841 gcgatggcgt cgcggatcga ctctgcgttg gacgaggcga tcttaattgt gccgtcgtcg
     6901 gagaggtcga tcttggcgcc ggactccgcc acgatctcgc ggatcacgga gccacccgaa
     6961 ccgatgactt cgcggatctt gtcgaccggg atgttgatcg tctcgatgcg cggtgcgtac
     7021 tcgccgagct ccggacgtgc gccggtcagc gccttgtcca tctcaccgag aatatgcatg
     7081 cgcccgccct tagcctggtc gagcgcgatc ttcatgatct cctcggtgat gccggtgatc
     7141 ttgatgtcca tctggaggga cgtgacgccc tcggacgtgc cagccacctt gaagtccata
     7201 tcgccgagat ggtcctcatc accaagaatg tcggacagaa cggcgaactt gtcgccttcc
     7261 ttgatgaggc ccatggcgat gccggcgcag ggcgccttca gcggcacgcc cgcgtccatc
     7321 agcgacagcg aggcgccgca gaccgaggcc atggaggacg agccgttcga ctcggtgatc
     7381 tccgagacga cgcggataac gtagccgaaa tcgtcgagat ccggcatgac cgcctggata
     7441 ccacgcttgg ccagctt
//
LOCUS       DBNHVU010000170         6600 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1845350, whole genome shotgun sequence.
ACCESSION   DBNHVU010000170 DBNHVU010000000
VERSION     DBNHVU010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1845350"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1392)
                     /gene="ntrX"
                     /locus_tag="AC16Y7_06950"
     CDS             complement(<1..1392)
                     /gene="ntrX"
                     /locus_tag="AC16Y7_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation response regulator NtrX"
                     /protein_id="HIR5709155.1"
                     /translation="MASDILVVDDEADIRELISGILEDEGHATRLAKDSDETLKQIED
                     RRPSLVILDIWLQGSKLDGLDLLDAIKKAHPDLPVIIISGHGNIETAVAAIQRGAYDY
                     IEKPFKVDRLVLITTRALEASRLKRENRELRERSTYSFEMVGKSAAIGQLRQAIEKVA
                     PTNSRVLITGPSGCGKELAARVMHAKSARADGPFVVLNAAAMVPDRMELELFGTEEGT
                     GSAPRKVGALEEAHGGTLYIDEVADMPLETQAKILRVLVEQRFQRLGGGTKVHVDVRV
                     VSSTSHDLQKEISAGRLREDLYHRLNVVPIRVPGLPERREDVPDLVRYFVQTISVASG
                     LPPRRIGEDAMAVLQSHEWPGNVRQLRNNVERLMILVGGDVDSIVTAEMLPDEIVSPV
                     PLSPNGAGGEHLMSLPLRDAREIFEREYLSAQVNRFGGNISRTAEFVGMERSALHRKL
                     RALGVNSNERRAAM"
     gene            complement(1382..3679)
                     /locus_tag="AC16Y7_06955"
     CDS             complement(1382..3679)
                     /locus_tag="AC16Y7_06955"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase NtrY-like"
                     /protein_id="HIR5709156.1"
                     /translation="MRPPSAGGPQEGPSGRRRLRFTLGLIIMVLALCSGLATYLILTG
                     LTPILPTQTVVVGVLLINSLLVLAMLALIAWQVAGLWIARRRHVAGAQLHIRIVSLFS
                     VIAIVPAILLAVFASVSLDRGLDHWFSSRTKSIIKNSIDVATAYLQEHGQVIRADALG
                     MAQDIDEAVELVKARPQGFGNFLKAQAAIRSLPGAFMIDEKGKVLATAASVPDFKYRA
                     PPEEAMGLAKKGQVIVIAPGQTSMVGAIKNLDNFDNTYLYVIRPVNARVLQQLRATRA
                     NVAEYQLLEQRRAGVQVAFGLMYVAIALTLLLSAIWIGMWFANRLVAPIGQLMGAAEE
                     ISHGNLGVEVAVNPADGDLATLGTSFNTMASELKTQRDELVGANATLDERNRFMEAVL
                     SGVTAGVVGVDADGTVNLVSRSAQDLLGVKEDKLTGKKLAEAVPEFGPHIKRAQKQDR
                     RLATDQITLRRAGSERNFAVRVTSEGAGQGYVVTFDDITELVSAQRSTAWADVARRIA
                     HEIKNPLTPIQLSAERIRRKYGSAITKDREIFDQCTDTIIRQVSDIGRMVDEFSAFAR
                     MPKPVMEMHDVREIVREAVFLFQVSRPDIEFQMDLPDKPVTTMSDQRLLTQAVTNLVK
                     NASEGIDSAVEADPARKDTGHITAKVGTKGDRIHITIIDNGCGLPKENRERLVEPYMT
                     TRVKGTGLGLAIVQRITEQHGGKIQLADAPKRNGKVEGASVRMDLPIVAREEAETDVE
                     SDDAPSAKASGSTPAEEEEGVTHGV"
     gene            complement(3901..5343)
                     /gene="ntrC"
                     /locus_tag="AC16Y7_06960"
     CDS             complement(3901..5343)
                     /gene="ntrC"
                     /locus_tag="AC16Y7_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HIR5709157.1"
                     /translation="MGKGNILIADDDAAIRTVLNQALARAGYAPRATGNAATLWRWIS
                     QGEGDLVITDVVMPDENAFDLIPRVKKLRPDLPIIVMSAQNTFMTAISAAERGAYEYL
                     PKPFDLNELVSVVGRALSEPRKRAPRLSAEPTDGMPLIGRCPAMQEIYRILARLTQTD
                     LTVMIAGESGTGKELVARALHEYGKRRNGPFVAINMAAIPRELIESELFGHEKGAFTG
                     AQTRHVGRFEQAEGGTLFLDEIGDMPMEAQTRLLRVLQEGEYTTVGGRTPIRTNVRIV
                     AATNRELQQQIDQGLFREDLYYRLNVVPLRLPPLRERLEDIPDLVRHFLSIAEKEGLP
                     PKGFEPAAIERLKRYHWPGNVRELENLVRRLSAVYAQDIVTDEMVESELATAARAPYS
                     ENAETTGDLSESVEHFLADYFMGFGQDLPPAGLYTRILRKVEFPLITAALAATRGNQI
                     RAADLLGLNRNTLRKKIRDLGIKVIRTPSA"
     gene            complement(5349..6449)
                     /locus_tag="AC16Y7_06965"
     CDS             complement(5349..6449)
                     /locus_tag="AC16Y7_06965"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR5709158.1"
                     /translation="MPCEVLLDGLPHPLLVVGEDHAITYVNSAAEDFFQMSAGVLCRS
                     TLPDVVAFGSPLVALVEQVERHGTTVNEYGVELGSFRFEAPKLVDLYGGPVGETPGSV
                     MVMLQQRSMAQMIERQLTHRGAARSVSGLAAVLAHEIKNPLSGIRGAAQLLESDVPEG
                     DRTLTQLICAESDRICKLVDRMEIFGDERPIQLEPVNIHDVLDHVKQVANTGFASHIK
                     IVESYDPSLPPVPGNRDKLIQVFLNLVKNAAEAIGEDRADGQITLTTSFRPGVRLSVP
                     GSKARVSLPLEIAVEDNGGGVSGELLPHLFDPFVSTKPSGTGLGLALVAKIIGDHGGI
                     IECDSHPRCTVFRALMPMAEKEAKQMIEATQA"
BASE COUNT         1132 a         1975 c         2159 g         1334 t
ORIGIN      
        1 catggcagct cttctttcat tggaattgac gccgagagcc cgaagtttcc ggtggagggc
       61 tgagcgttcc atgccgacaa attcggccgt gcgggagatg tttccgccga atcggttgac
      121 ttgggccgag agatactcgc gctcgaagat ttccctggcg tcgcgcaaag gcagcgacat
      181 caggtgttcg cccccggccc cgttgggcga gagcggcacg ggcgaaacga tctcgtcggg
      241 gagcatctcc gccgtgacga tgctgtcgac atcgccgccg acaagaatca tcaagcgttc
      301 cacgttgttg cgcagctggc ggacattgcc tggccattcg tgagattgga gcacggccat
      361 ggcgtcctcg ccgatgcgcc ggggcggcag gccggaggcg acggagatgg tttgcacgaa
      421 gtaccggacc agatccggca cgtcctcacg gcgttcgggc aggccgggca cacggatcgg
      481 caccacgttc aggcggtggt aaaggtcttc gcgcaagcgt ccggccgaga tctctttttg
      541 gagatcgtgg ctggtcgaag agaccacgcg cacgtccaca tgcactttcg ttccgccgcc
      601 gaggcgctga aagcgttgct cgacgagcac ccgcaagatc ttggcttggg tctcgagggg
      661 catgtcggcg acttcgtcga tataaagcgt gccgccatgg gcttcctcta gtgcgccgac
      721 cttgcggggg gcggagccgg tcccctcctc cgtcccgaac agttcgagtt ccatgcggtc
      781 cggcaccatc gccgcggcgt tgaggacaac gaacggccca tcggcgcgtg ccgacttggc
      841 gtgcatgacg cgggcggcga gttctttgcc gcagccggac ggtcccgtga tcaacacgcg
      901 gctattggtc ggggcgacct tctcgatcgc ctgacgcagc tggccgatgg ccgccgattt
      961 accgaccatc tcgaaagaat aggtgctgcg ttcgcgcagc tcgcggtttt cgcgcttcag
     1021 ccgggaggct tccagcgctc gcgtggtgat gagaaccagc cggtcgacct taaacggctt
     1081 ttcgatgtag tcgtacgcgc cgcgctggat ggccgccacc gcggtttcga tgttgccgtg
     1141 gccgctgatg atgatcaccg gaaggtccgg gtgggccttc ttgatggcat cgagcaaatc
     1201 aaggccatca agcttcgatc cttgaagcca gatgtcgagg atgacgagcg acggacgccg
     1261 gtcctcgatt tgcttcaaag tctcgtcgct gtccttggcg agacgtgtcg cgtgtccttc
     1321 gtcctcaagg atacctgaga tcagttccct gatatcggct tcgtcgtcga cgaccaagat
     1381 atcagacgcc atgggttact ccctcttcct cctcggccgg cgtggatccg ctcgctttgg
     1441 ccgaaggggc atcgtcggac tccacgtccg tctccgcctc ctcccgcgcc acaatcggca
     1501 ggtccattcg cactgacgcc ccttccactt tgccgttgcg cttgggcgca tcggccaatt
     1561 gtattttgcc gccgtgctgc tcagtgatgc gctgcacgat ggctagtcca agtcctgtgc
     1621 ctttgacgcg cgtggtcatg taaggctcga ccaggcgctc gcggttctct ttcggcaagc
     1681 cgcagccatt gtcgatgatc gtgatgtgga tgcggtctcc cttggtcccc accttcgccg
     1741 tgatgtggcc ggtgtctttg cgtgccgggt ccgcctctac cgcagaatca attccttcgc
     1801 tggcgttctt cacgaggttc gtcacagcct gggtaagcag gcgctgatca gacatggtcg
     1861 tgaccggctt gtccggcaag tccatctgga actcgatatc gggccggctt acctggaaca
     1921 ggaagacggc ctcgcgcacg atctcgcgca cgtcgtgcat ctccatgacc ggcttcggca
     1981 tgcgcgcgaa ggcggagaac tcatcgacca tgcggccgat atccgacact tggcggatga
     2041 tggtgtcggt acactggtcg aagatctcgc ggtccttggt gatggcgctg ccgtatttgc
     2101 ggcgaatacg ctcggcggac agctggatgg gcgtgagcgg gttcttgatc tcgtgcgcga
     2161 tgcggcgcgc gacgtcggcc caggccgtgc tgcgctgggc gctgacaagc tcggtgatgt
     2221 cgtcgaaggt cacgacatag ccctggcccg cgccttcgct ggtgacacgg acggcgaagt
     2281 tccgttccga gccggcccgg cgcagcgtga tctggtcggt ggcaaggcgc ctgtcctgct
     2341 tctgggcccg tttgatgtgc gggccgaatt ctggcacggc ctccgccagc ttcttgccgg
     2401 tgagtttgtc ttccttgacg cccaaaagat cctgcgcgga gcggctcacg agattgaccg
     2461 tgccgtcggc gtcgactccg accacgcctg cggtgacgcc ggacagcacc gcctccatga
     2521 agcggttgcg ttcatcgagg gtcgcgttgg cgcccaccag ctcgtcacgc tgggtcttga
     2581 gctcggaggc catggtgttg aacgaggtgc cgagcgtcgc aagatcgccg tcggctggat
     2641 tgacggcgac ctcgacaccg agattgccgt ggctgatttc ttcggccgcg cccatcaact
     2701 gaccgatcgg ggcgacaagg cggttggcga accacatacc gatccagatg gccgacaaga
     2761 gtagcgtgag cgcgatcgcc acatacatga gcccgaaagc aacttgcacg ccggcgcggc
     2821 gctgctcgag caattgatac tcggctacgt tggcgcgcgt agcgcggagt tgttgcagta
     2881 cgcgcgcgtt cacgggccgg atgacgtaga gatatgtgtt gtcgaagttg tccaggttct
     2941 tgatcgcgcc caccatgctg gtctgccctg gcgcgatcac gatgacttgg cccttcttgg
     3001 cgagccccat ggcctcttcc ggcggtgccc ggtatttgaa atcggggacc gacgcagcgg
     3061 tcgcgagcac cttgcctttc tcgtcgatca taaaggcgcc cggaagcgat cgaatggcgg
     3121 cttgggcctt gaggaaattg ccgaagcctt gcgggcgcgc cttgacgagt tccacggcct
     3181 catcgatgtc ctgcgccata ccgagtgcat cggcgcggat cacctgtccg tgctcttgca
     3241 agtaagccgt ggcgacgtcg atcgagttct tgatgatcga tttggtgcgt gatgagaacc
     3301 agtgatcgag gccgcggtcg agcgacacgc tggcgaagac ggcaaggagg atcgccggca
     3361 cgatggcgat cacgctgaac aggctgacaa tgcgaatgtg aagttgcgcg ccggccacgt
     3421 ggcggcgtct ggcgatccat agcccagcga cctgccaggc gatgagcgcc agcatggcaa
     3481 ggacgaggag cgagttgatg agcagcaccc caacgaccac cgtttgggtg ggcaggatgg
     3541 gggtcagacc ggtgaggatg agataggtcg cgaggcctga gcacaacgcc aggaccatga
     3601 tgatcaggcc gagcgtgaaa cgcaggcggc ggcggccgct tgggccttct tgcgggccac
     3661 cggcagatgg cgggcgcaaa ggtcccttgt ttttctgttg ttctgtagag gtttggaaga
     3721 cgctgctcaa ccgttttccc ctgggtctcc tgcaggcaaa cccgggcgca ccgggacttg
     3781 ccgcgtggcg cgtccgcctc agctgtggtg caataaaatc acaatgtggc gctttggcga
     3841 catgctagcc acaacctcag ccgtttggag atatgggggc ctgcgcccga attgaccagg
     3901 ctaagcgctc ggcgtgcgga tgaccttgat gccgaggtcg cggatcttct tgcgcagcgt
     3961 attgcgattg agtccaagga gatcggcagc gcgaatttgg ttgcctctgg tggcggcaag
     4021 cgcggcggta atcagcggaa actccacttt gcgcaagatg cgcgtgtaaa ggccagcggg
     4081 gggaaggtcc tgaccgaaac ccatgaagta gtctgcgaga aaatgctcca cggactccga
     4141 aaggtcgccg gtggtttcgg cgttctcgga atacggtgcg cgggcggcag tggccagctc
     4201 gctttccacc atctcgtcgg tgacgatgtc ttgggcatag accgccgata gacgcctcac
     4261 caagttctca agctcgcgca cattgcccgg ccagtggtag cgcttgagcc gttcgatggc
     4321 ggcgggctcg aagcccttgg gcggaagccc ttccttctcc gcgatggaga gaaagtggcg
     4381 cacgaggtcg ggaatatctt caagacgttc gcgcaagggc ggcagcctga gcggcacgac
     4441 gttcagccgg taatagagat cctcgcggaa cagaccctgg tcgatttgct gctgcagttc
     4501 acggttggtc gcggcgacga tccggacatt ggtgcggatt ggggtgcggc cgccgacggt
     4561 ggtgtactcc ccctcttgca gcacgcgtaa gagtcgcgtc tgcgcttcca ttggcatgtc
     4621 gccgatctcg tcgagaaata gcgtaccgcc ttcggcttgt tcgaagcgtc cgacgtgacg
     4681 tgtctgcgcg cccgtgaagg cgcccttctc gtgcccgaac aattccgact cgatcagctc
     4741 gcgggggatc gccgccatgt tgatcgcgac aaacggaccg ttgcgcctct tgccgtattc
     4801 atggagcgcg cgggcgacga gctctttgcc cgtgccggac tcgcccgcga tcatcaccgt
     4861 cagatcggtt tgggtcagac gcgccaggat tcggtaaatc tcctgcatgg ccggacaccg
     4921 gccgatgagg ggcatgccgt ctgtcggctc ggcggacaga cgcggagctc gcttgcgcgg
     4981 ctcggacaaa gcgcggccga cgactgagac gagttcgttc agatcgaatg gtttcggcag
     5041 atactcgtag gcgccacgtt cagccgcgct gatggcggtc atgaaggtgt tctgcgcgct
     5101 catgacgatg atgggcagat ccggccgcag cttcttaaca cggggaatga ggtcgaacgc
     5161 gttctcgtcc ggcatgacga cgtccgtgat caccaagtcc ccttcccctt ggctaatcca
     5221 gcgccacagc gttgcggcat tgccggttgc gcgcggggca tagccggcgc gggccagtgc
     5281 ttggttgaga actgttcgga tcgcagcgtc gtcgtccgcg atgaggatat tgcctttgcc
     5341 catttttctt atgcctgcgt tgcttctatc atctgcttgg cttccttctc ggccatgggc
     5401 atcagcgccc gaaagaccgt gcatcggggg tgtgagtcgc attcgatgat tcccccatgg
     5461 tcgccaatga tcttggccac gagggccagt ccgagccccg tgcctgacgg cttggtggag
     5521 acaaacggat cgaaaagatg cggcaggagt tcgccggaca ccccgccgcc gttgtcctcg
     5581 acggcgattt cgaggggcag actgacacgc gccttgctgc cgggaaccga gagccgcacg
     5641 cccgggcgga acgacgtggt cagcgtgatc tggccgtcgg cccgatcttc gccgatggcc
     5701 tcggcggcgt tcttcacgag attgaggaag acctgaatga gcttgtctcg gttgccgggg
     5761 acaggcggca gcgacgggtc gtagctctct acgatcttga tgtgactagc gaagccagtg
     5821 tttgccacct gcttcacgtg gtcgagcacg tcgtggatgt tgaccggttc cagttggatg
     5881 ggccgctcgt cgccgaagat ctccatgcgg tcgacgagtt tgcagatgcg atcggattcc
     5941 gcacagatca gctgggtcaa ggtccggtcg ccttccggga cgtcgctctc caggagctgc
     6001 gcggctccgc gaatgccgga cagcgggttc ttgatttcat gcgcaaggac ggccgcaaga
     6061 ccggacacgg aacgtgcggc gccgcgatgg gtgagttgcc gctcgatcat ctgcgccatg
     6121 ctgcgctgct ggagcatgac catgacgctg cccggcgtct cgccaacagg accgccgtag
     6181 agatcgacga gcttcggcgc ttcgaagcgg aacgacccaa gttccacgcc atactcgttg
     6241 accgtggtgc catggcgctc cacttgctcg acgagtgcga ccagggggct gccaaaggcg
     6301 acgacgtccg gaagcgtgct tcggcataaa acgccggcgc tcatttgaaa gaagtcctcg
     6361 gcggccgagt tcacgtaagt gatggcgtgg tcttcgccga cgaccaggag cggatggggc
     6421 aagccatcga gaagcacctc gcaaggcatg cccctcgcct gctccttgcg tcggaagccg
     6481 gggtttttgg tcacggggct caaagcctat atacctcgcg gtctcgccat gcccgtcgcg
     6541 gatgcgcaaa agttgggcac ccgtagtcac tgatctatta ttaggcaaga tacgagtgtt
//
LOCUS       DBNHVU010000171         8276 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1400459, whole genome shotgun sequence.
ACCESSION   DBNHVU010000171 DBNHVU010000000
VERSION     DBNHVU010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8276)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8276)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8276
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1400459"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /gene="tsf"
                     /locus_tag="AC16Y7_06970"
     CDS             complement(<1..325)
                     /gene="tsf"
                     /locus_tag="AC16Y7_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR5709159.1"
                     /translation="MATITAALVKELRDKTGAGMMDCKAALNENDGDMEAAIDWLRAK
                     GLAKAAKKAGRVAAEGLIGLAGDDKEAAVVEVNSETDFVARNPSFQELVQTIAAAGPK
                     AKGDLA"
     gene            complement(371..1312)
                     /gene="rpsB"
                     /locus_tag="AC16Y7_06975"
     CDS             complement(371..1312)
                     /gene="rpsB"
                     /locus_tag="AC16Y7_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR5709160.1"
                     /translation="MALPDITMRQLLEAGVHFGHQTHRWNPKMAPFIYGSRNNIHIID
                     LTQTVPLLHQALLTVSDVVAKGGRVLFVGTKRQASEPIADAARSSAQYFINHRWLGGT
                     LTNWRTISNSIKRLKSLEELFEGEHHGLTKKELLQLTREKDKLERSLGGIKDMGAPPD
                     LMFVIDTNKESIAIAEARKLKMPVVAIVDSNCDPDGITFPIPGNDDAGRAITLYCDLI
                     SRAAIDGIERAQGSAGMDVGAAEEPLAEPGLEGEDAPKADAKPEPKKAEKKPAKAKAP
                     AKRKPAAKKKAETSDAETPDSEAKGSGAKASGAEEEA"
     gene            complement(1545..5009)
                     /gene="dnaE"
                     /locus_tag="AC16Y7_06980"
     CDS             complement(1545..5009)
                     /gene="dnaE"
                     /locus_tag="AC16Y7_06980"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR5709161.1"
                     /translation="MTTRPQPFVHLRVHSAYSLLEGALKIPKLVELAKADHAPALALT
                     DANNLFGALEFSEAMANAGIQPIIGCTLTLTFEQEPEPAPGTGPGAPKADGRIALLAK
                     DETGYANLMRLSTQAYFAAAETGEALVTVEQLSEHASGLIALTGGPEGVIDLALADGN
                     PEFAKSRLEALHALFADRLYVELQRHGLPQEKGTEPHLIGLAYDMELPLVATNEPFFA
                     QHDDFEAHDALICIAQGSYVAVDERRRLSPEHHFKTAEQMRALFADIPEALENTVEIA
                     RRCAYRPMLRDPILPAFLSGEQATSALAETEAEELRRQAETGLAERLAQTGTAPGHDE
                     AAYKDRLAFELDVITGMNYQGYFLIVSDFIKWTKGQGIPVGPGRGSGAGSLVAYALTI
                     TDLDPIRFGLLFERFLNPERVSMPDFDIDFCQDRRDEVIAYVRERYGADRVAHIITFG
                     KLQARAVLRDVGRVLQMPYGQVDRLCKLVPMNPANPVTLPQAIAGEPRLQEERDKEPI
                     VAKLLDIGQRLEGLYRHASTHAAGVVIADRPLIDLVPLYRDSRAQLPATQFNMKWAEA
                     AGLVKFDFLGLKTLTVIDTARLLIARDGPDIDPAKIPLDDEASFELMQRGDTIGVFQL
                     EGQGMRDALRKLKPDRFEDIIAMVALYRPGPMDNIETFVNRKHGREEIESLHPMIEPI
                     LEETYGVIIYQEQVMQIAQVLSGYSLGEADLLRRAMGKKIKKEMDAQRKRFVDGAVEN
                     GVDKGRAEYIFELVAKFAGYGFNKSHASGYALIAYQTAYLKANYPTEFIAASMTLDMG
                     NADKLAGFAQEAERLGIGIEPPSLNHSEVGFIPSGGAIRYSLAALKNVGRQAVESICA
                     ERDENGPFKSVPDFAQRLNPRLVNKRALETLSAAGAFDDLDIDRATAHANADLIIATG
                     NSAQEARAEGQNDLFSGGGAAPEITLRRANPWGSTDRLSMEFDAVGFFLSGHPLDDYS
                     EVLQNIGVEPWTEFEGKARARRVVGKLSGTVLSARERTGKSGNPYAFVVFSDPTGQFE
                     AVIFSEALVAGRHLLETGTAVLLDVEAEADDDTVRVRIQGLESLDNAAQTKASGMRVY
                     VESPKALAPLAKQVGAQGGGGAFRLVLRLDELAKEVEFELPRGVDTTPRQRSELKMVE
                     GVVSISAL"
     gene            complement(5176..5895)
                     /locus_tag="AC16Y7_06985"
     CDS             complement(5176..5895)
                     /locus_tag="AC16Y7_06985"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5709162.1"
                     /translation="MDNPERTPALRLENLDRGFTQGDRRIDVLQKACATFYPGETVAL
                     LGPSGAGKSTLLHIAGLLERPDEGAVIINGIDCGSLSDNERTRIRRLDVGFVYQFHHL
                     LPEFSALENVMLPQLILGQDKPDAEARAKNLLKTLGLEQRWEHRPAELSGGEQQRVAI
                     ARAVANQPKVLLADEPTGNLDPHTAERVFAELMKLVTEEGVAAVVATHNLELAARMDR
                     TLRLTDGHLIEEDVVVVETAS"
     gene            complement(5905..7182)
                     /locus_tag="AC16Y7_06990"
     CDS             complement(5905..7182)
                     /locus_tag="AC16Y7_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013948495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HIR5709163.1"
                     /translation="MSATTSTPSGTRAFAPFEWLVAGRYLRARRKEGFISVIAGFSFL
                     GIMLGVATLIIVMAVMNGFRQELFDKMLGLNGHVVVHSLGEFTDYDEVAERVRKVDGV
                     KYALPLVEGQVLISTHYNSSGALVRGLREEDLKNLKAISNNIRFGTLDGFDGKPGLAL
                     GSRMANDLNVKVGDEVNILTPRGPSTALGIAPRTKRYPVVAIFEIGMSEYDASIMFMP
                     LTEAQRYFSQPKKVTVLEVVLDSPDEVADLAPKIITAGGPTIYVSDWRQRNATFFTAL
                     QVERNVMFLILTLIVLVAALNIISGLIMLVKDKGRDIAILRTMGTTRGAIMRIFFIAG
                     ASIGIVGTIAGLILGVLVCLNIDRIRGLIGWLTDTELFPAELYYLSQLPADMDTGETA
                     TVVVMALVLSVLATVYPSWRASRLDPVEALRYE"
     gene            complement(7179..>8276)
                     /gene="proS"
                     /locus_tag="AC16Y7_06995"
     CDS             complement(7179..>8276)
                     /gene="proS"
                     /locus_tag="AC16Y7_06995"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR5709164.1"
                     /translation="RYDDYGAEMLRIQDRHERDMLYGPTNEELITAIFRDAVKSYKEL
                     PRILYHIQWKFRDEIRPRFGVMRGREFLMKDSYSFDLDKEAAEKSYNKMFVAYLRTFA
                     RMGLHAVPMRAETGPIGGDLSHEFIILADTGESQVYCHKDLVEMPAPPADLDYAGDLT
                     EYVHQRTALYAATDDVHDPKRFEQEVPADKRLSARGIEVGQVFYFGTKYSEAMGARVQ
                     GPDGDLVTVEMGSYGVGVSRLLGAIIEASHDEAGIIWPQAVAPFDVGLINLKAGDKDT
                     DAACEDLYAKLQAAGLEILYDDTDDRAGAKFAAMDLIGLPWQLVVGPKGLKAGEIEVK
                     ERATGARHSLTFDASLNMLVDKAEQAKREAA"
BASE COUNT         1447 a         2512 c         2680 g         1637 t
ORIGIN      
        1 ccgccaagtc gcctttggcc ttgggaccgg cggcggcgat ggtctgcacc aattcctgga
       61 acgacggatt gcgcgccacg aagtcggtct ctgaattgac ctccacgacg gctgcttcct
      121 tgtcgtcgcc ggcgagccca ataagccctt cggcggcaac tcggccggcc tttttggccg
      181 ccttggcaag gcccttcgcc cgcagccaat cgatcgcggc ttccatgtcg ccgtcattct
      241 cgttcaatgc ggccttacaa tccatcatgc ccgcgccggt cttgtcgcgg agctccttga
      301 ccagggctgc agttatcgtc gccatacgtt cctcattgtt gcgccagggt gttgcaccgg
      361 cgtcttcacg ttacgcttct tcctcggctc cagaggcttt ggctccagag cccttggcct
      421 cggagtccgg cgtctcggcg tcggacgtct cagccttctt ctttgcggca ggcttgcgct
      481 tcgcaggcgc cttggctttg gcgggtttct tttccgcctt tttcggctcc ggcttggcgt
      541 cggccttggg tgcgtcttcg ccttcgagac caggttcggc caggggctct tcggcggcgc
      601 ccacatccat tccggcggag ccttgggccc gctcgatgcc atcaatggcg gcgcgcgaaa
      661 tcagatcgca atagagcgtg atggcgcgtc cggcatcgtc gttgcccgga atgggaaacg
      721 tgatgccgtc gggatcgcag ttggaatcga cgatggccac cacaggcatt ttgagcttgc
      781 gggcctcggc gatggcgatt gactccttgt tggtgtcgat cacgaacatc aggtcgggcg
      841 gtgcacccat gtccttgatg ccaccaaggg accgctcgag cttgtccttc tcacgggtga
      901 gctgaagcag ctccttcttc gtcaggccgt ggtgttcgcc ttcaaaaagc tcctccagcg
      961 acttgagccg cttgatggaa ttggaaatgg tccgccagtt ggtcagcgtg ccgccgagcc
     1021 aacggtgatt gatgaagtac tgcgcggacg agcgggccgc gtcagcgatc ggttcggagg
     1081 cttggcgctt ggtgccgacg aagagcacgc ggccaccctt ggccacgaca tcggacaccg
     1141 tgagcagtgc ctgatgcaga agcggcaccg tctgggtcag atcgatgatg tggatgttgt
     1201 tgcgggagcc gtaaatgaac ggcgccatct ttgggttcca gcgatgggtc tggtgaccaa
     1261 agtgaacgcc agcctccagg agctggcgca tagtaatatc gggcagtgcc atgggactgc
     1321 tgaactcctt tttccggtta tgccgccgcg gggcgaagag accggccgga caccggtcac
     1381 cggaacgagc gtgaaggcta tggggccttc gtgacgctaa tccccgcgtg tgagatggtg
     1441 cgcgttatag gggtgagacc ggcgcttggc aaggcccgag gcgcgagtcg agaccgaggc
     1501 cgtgatatgg tcggaaatgg ttgaaaagcc aagcgccgca acggctacag ggcgctgatc
     1561 gagaccacgc cctccaccat tttgagctcg ctgcgctgac gcggcgtggt gtcgacaccc
     1621 cggggcagtt cgaactccac ctccttggcg agttcgtcga ggcgcagcac gaggcggaac
     1681 gcgcccccgc ccccttgcgc cccaacctgt ttggccaggg gcgcaagcgc cttcggactc
     1741 tccacataga ccctcatgcc ggaagccttg gtctgcgcgg cgttatcgag agactctagg
     1801 ccttgaatgc ggacgcgaac ggtgtcgtcg tcggcttccg cctccacatc caggaggacg
     1861 gctgtgccgg tctcaagcag atgccggcca gcgacaaggg cctcggaaaa gatgaccgcc
     1921 tcgaactggc ccgtgggatc tgagaacacg acaaaggcat aagggttgcc gctcttgccc
     1981 gtgcgctccc gggccgaaag gacggtgccc gacagcttgc cgaccacgcg gcgggctcgc
     2041 gccttgcctt cgaattcggt ccaaggttcg acgccaatgt tttgtaaaac ctcgctataa
     2101 tcgtctagcg gatgtccgct gagaaagaac ccgacggcgt cgaactccat ggagaggcgg
     2161 tccgtcgagc cccacggatt ggcgcggcga agcgtgattt ccggcgcggc gcccccgccg
     2221 gaaaacaggt cattctgccc ttcggcccgg gcctcttggg cgctattgcc ggtggcaatg
     2281 atcaagtcgg cattggcatg ggccgtggcc cggtcgatat caagatcatc gaacgcgcct
     2341 gccgccgaca gcgtttccaa ggcgcgctta ttgacaagac gcgggttaag ccgctgcgcg
     2401 aaatccggca cactcttaaa cgggccattc tcgtcgcgct cggcgcaaat ggactccacg
     2461 gcttggcggc cgacattctt gagcgcggcg agtgaatagc ggatggcacc gccgctgggg
     2521 atgaagccaa cttccgaatg attgagggac ggcggctcga tcccaatgcc caatcgctcc
     2581 gcttcctgcg cgaagccggc cagcttatcg gcattgccca tgtcgagggt catggatgcg
     2641 gcgatgaact ctgttggata gttcgctttc agataggccg tctggtaggc gatcagcgca
     2701 tagccggagg catgagactt gttgaagcca tagccggcga acttcgcgac cagctcgaaa
     2761 atgtattccg cgcggccctt gtcgacgccg ttctccacgg ccccgtcgac gaagcgcttc
     2821 cgctgggcgt ccatctcctt cttgatcttc ttgcccatgg cgcggcgcag gagatcggcc
     2881 tcgcccaggg agtagccgga taggacttgc gcgatctgca tcacctgctc ctggtagatg
     2941 atgacgccgt aggtctcctc caggatcggc tcgatcatgg gatgcaggct ttcgatctcc
     3001 tcgcgcccgt gcttgcggtt cacgaaggtc tcgatgttgt ccatcgggcc cggtcgatag
     3061 agcgcgacca tggcgatgat gtcctcgaag cggtcgggct tcagcttgcg caaggcatcg
     3121 cgcatgccct gtccttcaag ctggaacacg ccgatcgtgt cgccacgctg catgagttcg
     3181 aaggacgctt catcgtccaa gggaatcttc gccggatcga tgtcgggccc atcgcgggca
     3241 atcaacagcc gcgcggtgtc gatgaccgtg agcgtcttca ggccgaggaa gtcgaacttc
     3301 accagccctg cggcctcggc ccacttcatg ttgaactgcg ttgccggaag ctgggcgcgc
     3361 gaatcccgat agagcgggac caggtcgatt aagggccggt cggcgatcac cacgcccgcg
     3421 gcgtgggtgg aggcgtgccg ataaaggccc tccagccgct ggccgatgtc gagcagcttg
     3481 gcgactatcg gctccttgtc gcgttcctct tgcaagcgcg gctcgccggc gatggcttgc
     3541 ggcagcgtca ccgggttcgc cgggttcatc ggcaccagct tgcacagccg gtcgacttgg
     3601 ccatacggca tttgcaggac acggccgacg tcgcgcagca cggcgcgcgc ttgaagctta
     3661 ccgaaggtga taatgtgggc gacgcggtcg gcgccatagc gttcgcgcac ataggcgatc
     3721 acctcgtcgc ggcggtcctg gcagaaatcg atatcaaagt ccggcatgga cacgcgttcg
     3781 ggattcaaga agcgctcgaa caggagaccg aagcggatcg gatcgaggtc ggtgatggtg
     3841 agcgcgtaag ccacaagcga gccggcgccc gagccacggc cggggccgac gggaatgccc
     3901 tgccccttgg tccacttgat gaagtccgaa acgatcagga agtagccctg atagttcatg
     3961 ccggtgatga catcgagctc gaaggcgaga cggtccttgt aggccgcttc atcatggccc
     4021 ggtgcggtgc cggtttgtgc gaggcgctct gccaatccgg tttcggcttg ccggcgcagt
     4081 tcctccgcct ccgtttcggc gagcgcgctg gtggcttgtt cgccggagag aaaggctggc
     4141 aggatcgggt cgcgcagcat cggcctgtag gcgcagcgcc gggcgatctc caccgtgttc
     4201 tcaagcgcct ccgggatatc ggcgaacaac gcgcgcatct gctcggcggt cttgaagtgg
     4261 tgctcggggc tcaaacgcct gcgttcgtcg acggcgacat agcttccttg cgcgatacaa
     4321 atcagcgcat cgtgcgcctc gaaatcatcg tgttgcgcga agaacggctc gttggtggca
     4381 acgagcggca gttccatgtc gtaggcaagg ccgatgagat ggggctcggt tcccttttcc
     4441 tgtggcagtc catgacgctg cagctccacg tagagccgat ctgcgaagag cgcgtgcaaa
     4501 gcctccagac ggcttttggc aaactccggg tttccatctg cgagggcgag gtcgatcacc
     4561 ccttccggac cgccggtcaa cgcgatcagc ccactcgcgt gctctgacag ctgctcgacg
     4621 gtgacgagcg cctcgccggt ttcagccgcg gcgaaatagg cttgggtcga aagccgcatt
     4681 agattggcgt agccggtctc gtctttggcg aggagcgcga tgcggccgtc cgctttgggt
     4741 gcgcctgggc cggttcctgg tgccggctcc ggctcttgct cgaaggtcag tgtcagcgtg
     4801 cagccaatga tgggctggat gcccgcgttc gccatggctt cggagaattc cagcgcgcca
     4861 aataggttgt tggcgtcggt gagcgcgagc gccggcgcgt ggtcggcctt cgccagctcg
     4921 acaagtttgg ggatcttcaa cgccccctcc agaagggagt aggcggagtg cacgcgaaga
     4981 tgcacgaagg gttgggggcg cgtcgtcatt ggtcctcttg ccgcgatctc gtcccagctt
     5041 ttcggattcg gggtctcaga tcgattggcg tatccgaaag tccccaggat tcacggtttc
     5101 gcggcaggga ccccgagaat ctaccgttag atgttcttat tctgttctgg cgccagggac
     5161 ttggcaaggg gaggattagc tggcggtctc caccacgacc acgtcctcct cgatgaggtg
     5221 accgtcggtt aggcgtagcg tgcggtccat gcgggccgcg agctccagat tgtgggtcgc
     5281 caccacggcg gcgacgccct cttctgtgac aagcttcatc agctccgcga agacccgctc
     5341 ggcggtgtgg ggatcgagat tgcccgtcgg ctcgtccgcc aagaggacct tgggctggtt
     5401 ggcgacggcg cgggcaatgg ccacgcgttg ctgctcgccg ccggacagtt ccgccgggcg
     5461 atgctcccag cgctgttcca ggcccagcgt cttcagcagg ttcttggcgc gggcctcggc
     5521 gtcgggcttg tcctggccga gaatgagctg cggcagcatg acgttctcca gggccgagaa
     5581 ctcgggcagc agatgatgga actgatagac gaagcccacg tccaggcgcc ggatgcgcgt
     5641 ccgctcattg tcggacagcg agccgcagtc gatgccattg atgattactg cgccctcgtc
     5701 cgggcgctcc aggagaccgg cgatgtgcag cagcgttgac ttgcccgcgc ctgacgggcc
     5761 cagcagcgcc acggtctcgc cgggatagaa ggtggcgcac gccttctgga gcacgtcgat
     5821 gcggcgatca ccttgggtga agccgcggtc gaggttctca agccgcagag ccggggttcg
     5881 ttcgggattg tccaagaaag ccccctactc gtagcgcagt gcttcgaccg gatcgagacg
     5941 cgatgcccgc caggacggat agaccgtggc gagcaccgat aacacaagcg ccatgacgac
     6001 aacggttgcc gtctcgcccg tgtccatatc ggccggaagt tgagacagat aatagagctc
     6061 cgccggaaag agttcggtgt cggtcaacca gccgatgaga cctcggatgc ggtcgatgtt
     6121 gagacacacg agaacgccga ggatcagtcc ggcgatcgtc ccgacgatgc cgattgaagc
     6181 gccagcgatg aagaaaatgc gcatgatcgc gccgcgtgtc gtgcccatgg ttcgtaagat
     6241 cgcaatgtcg cggcccttgt ctttcaccag catgatgagg ccggaaatga tgttgagcgc
     6301 cgccaccagc acgataagcg tcagaataag gaacatgacg ttgcgctcca cttgaagcgc
     6361 agtgaagaac gtcgcgttcc gttgccgcca gtcgctgaca tagatggttg ggccgccggc
     6421 ggtgatgatc ttcggcgcta ggtctgcaac ctcgtctgga gagtcgagca cgacttcgag
     6481 caccgtcact tttttgggct ggctaaaata acgctgcgcc tcggtgagcg gcataaacat
     6541 gatgctcgcg tcgtattcgg acatgccgat ctcgaagatg gcgacgaccg gatagcgctt
     6601 ggtgcgcggg gcgatgccga gcgccgtgga tggaccgcgg ggcgtcagaa tgttgacttc
     6661 gtcgccgacc ttcacgttga ggtcgttggc catgcggctg cccagcgcca gacccggctt
     6721 tccgtcgaac ccatccaaag tgccgaagcg gatattgttg gaaatggcct tgaggttttt
     6781 gaggtcctct tcgcgtaagc cgcgcacgag cgcgccgctg gaattgtagt gcgtggagat
     6841 cagcacctgt ccctccacca agggtagtgc gtatttcacg ccgtcgactt tgcggacgcg
     6901 ctcggccacc tcgtcatagt cggtgaactc gccgaggcta tggacgacca cgtggccgtt
     6961 aaggccgagc atcttgtcga acagctcctg gcggaagccg ttcatcaccg ccatgacgat
     7021 gatcagcgtg gcgacgccga gcatgatgcc gaggaaggag aagccggcga tcacggagat
     7081 gaagccttcc ttccgccgtg cccgcaggta acgccccgcc accagccatt cgaagggtgc
     7141 aaaagcccgc gtgccgcttg gagtgcttgt ggtggcgctc atgccgcctc cctcttggcc
     7201 tgttccgcct tgtcgacgag catattaagg ctcgcgtcga aggtaagact atggcgggcg
     7261 ccggtggcgc gttccttcac ttcgatttct ccggccttca ggcccttggg ccccacgaca
     7321 agctgccagg ggagcccgat caggtccata gcggcgaatt tagccccagc ccggtcatcg
     7381 gtgtcgtcat agagaatttc gagccccgcc gcttggagct tcgcgtagag gtcctcgcaa
     7441 gccgcatcgg tgtccttatc gccggccttc agattgatca ggccgacatc gaagggggcg
     7501 acggcttgcg gccagatgat cccggcctcg tcatgggacg cctcgatgat agcgccgagc
     7561 agccgtgaga cgccgacgcc gtaggagccc atctcgaccg tcaccaggtc gccgtccgga
     7621 ccttggacgc gcgcgcccat ggcttcggag tacttcgtgc cgaaatagaa aacttgcccg
     7681 acctcgatac cgcgagcgga gagtcgcttg tcggcgggca cctcctgctc gaagcgcttc
     7741 ggatcgtgca cgtcgtcggt cgccgcgtag agcgcggtgc gctgatggac atattcggtg
     7801 agatcgcctg catagtcgag atcggccggg ggcgccggca tctccaccag atccttgtga
     7861 caatagacct gggactcgcc ggtgtcggca aggatgatga attcatgaga gagatcgccg
     7921 ccgatcggcc cagtctccgc gcgcattggt accgcgtgaa gtcccatgcg ggcgaacgtg
     7981 cgcaaatagg cgacgaacat cttgttgtac gatttctcgg cggcctcttt gtcgaggtcg
     8041 aaggagtaag aatccttcat gaggaattcg cggccgcgca tcacgccgaa gcgcgggcgg
     8101 atctcatctc ggaacttcca ttggatgtga tagaggatgc gcggcagctc tttgtaggac
     8161 ttcaccgcgt cgcggaagat ggccgtgatc agttcttcgt tggtcggccc atacagcatg
     8221 tcgcgttcgt ggcggtcctg gatgcgcagc atctcggcgc cgtaatcgtc atagcg
//
LOCUS       DBNHVU010000172         5146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_360407, whole genome shotgun sequence.
ACCESSION   DBNHVU010000172 DBNHVU010000000
VERSION     DBNHVU010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_360407"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            435..1550
                     /locus_tag="AC16Y7_07000"
     CDS             435..1550
                     /locus_tag="AC16Y7_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5709165.1"
                     /translation="MVSIGYLKQELEPFVPLSRLNVKPEDLGVAGAKIALRDNNTTGK
                     FTNQHFTLDVERVPVGGNAVAALKKLVEAGHHFVLVDAPADVLLKLSDAVKGKDVLLF
                     NVSAPDIRLRQEDCRANVLHTAPDLAMLADALAQYLVWKKWSRWLVAKGPFPEDQAYL
                     EAIRRSAKRFGATIVEERPYKATPGARRTDTGQQMIQLQMPVFTQGAPHYDVLIVADQ
                     REIFGPYLPFRTWDPRPVAGTAGLQAKSWHPAHEQWGATQMQNRFQRFADRFMLPLDH
                     QAWIAVRAIGEAASRTSSGAYASINTYIRGDRFDLAAFKGQKVTFRKWDGQLRQPLII
                     AGAELPVSTSPQKGFLHEHEETDTLGIDEPESKCQLQ"
     gene            1576..2553
                     /locus_tag="AC16Y7_07005"
     CDS             1576..2553
                     /locus_tag="AC16Y7_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent catabolism-associated
                     beta-propeller protein"
                     /protein_id="HIR5709166.1"
                     /translation="MNTNAVKVLSFAAALAAFTMPAHAYTVYISNEKGNSLTVIDSET
                     LQVLTTIPVGQRPRGIILTKDGRRVLVCASDDDTVQVLDVDSREIVADLPSGPDPELL
                     NLHPSGNPVYIANEDDNLLTVVDLNTKEVLAEVEVGVEPEGVGVSPDGKTVVVTSETT
                     NMAHFIDTETFEITDNVLVGTRPRFAEFTADGKQLWVTSELAGSVTVIDAQTREVIKV
                     IKFEIPGVQQIYIQPVGVRVTKDSTKAFVALGPANRVAVIDAKTYEVLDYLLVGKRVW
                     QLGFTPDQKLLFATNGNSNDVSVIDVDSLKVVKSIPTGTAPWGVAISED"
     gene            2770..3600
                     /locus_tag="AC16Y7_07010"
     CDS             2770..3600
                     /locus_tag="AC16Y7_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HIR5709167.1"
                     /translation="MSLTFILWVAALQMAAMSLIMAGGWLLQRRTGNSGFVDAAWTFG
                     LGAVGAASALLVLPGGDGPTARQTIVAALVAAWSVRLGTHIVQRTRTIADDPRYAALM
                     NDWGAEAPRRLFVFLQQQAIASLPLALTIFVAAQNPAPFSRVQDWLGLIVIVAGIAGE
                     GLSDRQLRRFVATRKEGARVCDIGLWSWSRHPNYFFEWMFWLAFPMFAIDFSGSYPWG
                     WLGLVGPALMYGLLVHVSGVPPLELHMVAKHGDAYRAYQQRTSVFFPLPPRNTERSPA
                     "
     gene            3597..4625
                     /locus_tag="AC16Y7_07015"
     CDS             3597..4625
                     /locus_tag="AC16Y7_07015"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR5709168.1"
                     /translation="MSLKAAATRIADHIPVPDAIPRFAIETFVRRTRASLPDDGEAAT
                     RDFAKTMLGRPIAAHPDAANAQHYEVPAEFFELVLGPRRKYSCCLYEGSDTTLAEAEE
                     LALAETLRRARIDQGMRILELGCGWGSLSLFMAERLPQSTILAVSNSHSQRASIEGLA
                     KARALDNLQVVTADVNAFDPGETFDRVISVEMFEHVSNWPELLSRVRPWLNPDGLLFL
                     HVFSHARVPYRFNHEDPNDWIAQHFFTGGIMPSHDLIGAFGAPFKVVETWRWNGRHYA
                     RTANDWLANMDRNEARVMEAFKPAYGEDARIWWRRWRLFFLATAGLFGHQAGEEWGVS
                     HHLLRPVS"
     gene            complement(4689..5132)
                     /locus_tag="AC16Y7_07020"
     CDS             complement(4689..5132)
                     /locus_tag="AC16Y7_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HIR5709169.1"
                     /translation="MVFTVSVAQAKSKDIVDTAAGAGQFKTLVAAVQAAGLVDTLKGD
                     GPFTVFAPTDEAFAKLPRGTVEDLLKPENKDKLVAILTYHVVPGKIMSSDIAGKSTEV
                     KTVQGDTLAVDATDGVKVDAANVVTADIGASNGVIHVIDTVVMPN"
BASE COUNT          958 a         1628 c         1556 g         1004 t
ORIGIN      
        1 ccggccgttt tcggccagaa gctccagacg ggcttgaagg gggaaaggtg cgcgccgcct
       61 tcgcagatgt caatgaatct atgaccaatc tccaacttcg gacttcgtat tcgcagcaaa
      121 ttctgccaac acttcccggc gaagctgcta cgtacagcgg cttggagcgg ctaaacttca
      181 cttcctaccg cgggtgggtt gccgcgcaga cgtccgcaag gagtcttttt gtgtcagtag
      241 gttatcctgt tccactccaa tggcccggtc ggctctcaag ctcagcgcgc cggacaaccc
      301 gttggctcgg acacctttgg tcaagagcgc tttttgcagg attcctcgga tttctactgt
      361 cggcccccgc gcctctttat gccaccgaag gcggggcctc acgggcctcg gacgctagcg
      421 cgccagagtc cactttggtc tccattgggt acctgaaaca ggagctggag ccattcgttc
      481 ccctgtcgcg attgaacgta aagcctgagg atttgggcgt cgcgggcgca aaaatcgcgc
      541 tgcgcgacaa caacaccacg ggcaagttca ccaaccagca cttcacactc gacgtcgaac
      601 gcgtgccggt cggtggcaat gccgtcgccg ctctcaagaa gctggtcgaa gccggtcatc
      661 actttgtgct ggtcgatgcg ccggcggacg ttctgctcaa gctctcagac gccgtcaaag
      721 gcaaagacgt acttctgttc aatgtgagcg cacccgatat ccgtctgcgc caagaagact
      781 gccgggcaaa cgtgcttcac accgcgcccg atctcgccat gcttgcggac gcgcttgcgc
      841 aatatctcgt ttggaagaag tggtcgcgct ggcttgtggc gaaaggcccc ttccccgaag
      901 accaagccta tctcgaagcg atccgccgct cggcgaaacg cttcggcgcc acgatcgtcg
      961 aggagcgacc ttacaaggcg acaccgggcg cacggcgcac tgataccggc cagcagatga
     1021 tccaactcca gatgcctgtc ttcacgcaag gcgcgcccca ttacgacgtg ctgattgttg
     1081 cagaccagcg ggagatcttt ggcccgtatc tgccgtttcg gacttgggac ccgcgtcccg
     1141 tcgccggcac ggcaggactt caagccaaga gttggcatcc ggcgcatgag caatggggcg
     1201 caacgcagat gcaaaaccgc ttccagcgtt ttgcagaccg gttcatgcta cctttggatc
     1261 accaagcctg gatcgctgtg cgcgccatcg gcgaagcggc ctcgcgcacc agttcgggcg
     1321 cgtatgcctc catcaacacc tatattcgcg gcgataggtt cgatctcgcc gccttcaagg
     1381 ggcaaaaggt gacgttccgc aaatgggatg gccaacttcg tcagcccctt atcatcgccg
     1441 gcgcggagct accggtttcg acatcgccgc agaaggggtt tctgcatgag cacgaagaaa
     1501 ccgatacgct tggcatcgat gagcccgaga gcaaatgtca gctccaataa gagaccgaac
     1561 cgccgggaga acgccgtgaa cacgaacgcc gtgaaagttc tgtcatttgc cgccgcgctt
     1621 gccgcattca cgatgcccgc gcacgcctac acggtctaca tctccaatga aaaaggtaac
     1681 tcgctgaccg tgatagacag cgagacattg caagtgctga cgacgatccc ggttgggcag
     1741 cggccacgcg gcatcatctt gaccaaggac ggccgccgcg tcctcgtctg cgccagcgac
     1801 gacgacaccg tgcaagtgct cgatgtggac tcaagggaga tcgtggcgga cctgccgtcc
     1861 ggacccgacc cggagctgct caacctgcac ccgtccggca acccggtcta tatcgccaac
     1921 gaggacgaca acctcctcac cgtggtcgat ctcaatacta aggaagtgct cgcagaagtc
     1981 gaggtcggcg tggaacccga aggcgtcggc gttagcccgg acggtaagac ggtggttgtg
     2041 acgtccgaga cgactaacat ggcccatttc atcgatacgg aaacctttga gatcaccgac
     2101 aacgtgcttg tcggcacgcg gccacgtttc gccgagttca cggcggacgg gaagcagttg
     2161 tgggtcacct cggaattggc gggctcggtc acggtgatcg atgcgcaaac gcgcgaagtg
     2221 atcaaggtca tcaagttcga gattccgggc gtgcagcaga tctacatcca gcccgtgggc
     2281 gtgcgcgtga ccaaggacag cacgaaggcc ttcgtggcgc tgggccccgc caatcgcgtc
     2341 gccgtcatcg acgcgaagac ttacgaggtc ctagactatc tgctcgtggg caagcgggtc
     2401 tggcagctcg gcttcacgcc agaccagaaa ctcctgtttg ccaccaacgg caacagcaac
     2461 gacgtgtcgg tgatcgatgt cgacagcctg aaggtcgtca agtcaatccc gaccggcacc
     2521 gcgccatggg gcgtggcgat ctcggaagac tgaacggcgc gtagccgcaa tcccgctagc
     2581 ttttgatcgc gtcattgaag ctcttggcgg ccgtctcgag cgacttggtg aaggcgttgc
     2641 gggactctat ctagcgcctg gctcatcgcg acccatgacg cctgtggcat ctgcccttgc
     2701 cgaattgatc cgggcggcca ctgttcgcgt aacccgcgca tagagagcgc ttgtcaggag
     2761 gtagccggct tgagcttaac gttcatcctg tgggtggcgg cgctgcaaat ggcggccatg
     2821 tccctgatca tggcgggggg atggctcctg caacggcgga caggaaacag cgggtttgtt
     2881 gacgctgctt ggaccttcgg gcttggcgcg gtcggcgctg cgtccgcgct cctggtccta
     2941 ccgggaggcg acgggccaac ggcgcgccag accatcgtcg ccgcgcttgt cgccgcctgg
     3001 agtgttcggc ttggaaccca catcgtgcag cggacccgca cgatcgccga tgaccctcgc
     3061 tatgccgctc tgatgaacga ctggggtgcg gaggccccga ggcgtctgtt cgtgttcctg
     3121 cagcagcaag cgattgcctc gctacccctg gcattgacca tcttcgtcgc cgcgcagaac
     3181 ccggccccat tctcgcgggt gcaggactgg ctcggtctga ttgtgatcgt cgccggcatt
     3241 gccggcgaag gactctcgga ccggcagctt cgccgcttcg ttgcgacacg gaaagaaggc
     3301 gcgcgcgtct gcgacatcgg cctctggagc tggtcgcggc atcccaacta cttcttcgag
     3361 tggatgttct ggctcgcctt ccccatgttc gccatcgact tcagcggctc gtatccgtgg
     3421 ggctggcttg ggcttgtagg ccccgccctc atgtatggcc ttctcgtcca cgtttcgggt
     3481 gtcccaccgc tcgagctgca tatggtggcc aaacacggcg atgcatatcg cgcttaccag
     3541 cagcgtacga gcgtcttctt tccgctgcca ccgcgcaaca ccgagaggag cccagcatga
     3601 gcctcaaagc cgcggccacc cggattgccg atcatatacc ggtccccgat gccatcccgc
     3661 gcttcgccat cgagacgttt gttcgccgga cgcgggcttc gttgccggat gacggagagg
     3721 cggcaacgcg cgatttcgcc aaaaccatgt tgggacggcc catcgccgcg caccccgatg
     3781 cggccaacgc tcagcactac gaggtgccgg ccgagttctt cgagctcgtg ctgggtccac
     3841 ggcgcaaata ctcttgctgt ctctacgagg gcagcgacac caccttggcc gaggccgagg
     3901 aactggcctt ggccgagacg ctccgccgcg ccaggatcga tcaaggcatg cgaattttgg
     3961 agctcggctg cggctggggc tcgctttcgc tgttcatggc cgagcggctg ccgcaaagca
     4021 cgatcctcgc cgtgtctaac tcacactccc agcgagcctc tatcgaaggc ctagcaaagg
     4081 cccgcgcgct cgacaatctt caggtcgtca ccgcggatgt gaacgcgttc gatcccggcg
     4141 agacgttcga tcgcgtgatc tcggtggaaa tgttcgagca tgtctcaaac tggcccgagc
     4201 tgttgtcgcg cgtgcggcca tggctcaatc cggatggcct cctgttcctt catgtgttca
     4261 gccatgctcg cgtgccctat cggttcaacc acgaggaccc caacgactgg atcgcgcagc
     4321 acttcttcac cggtggcatc atgcccagtc acgatttgat cggtgcgttc ggcgctccgt
     4381 tcaaagtcgt tgagacctgg cgctggaacg ggaggcacta cgcacgcacc gcgaacgact
     4441 ggttggccaa catggatcgc aacgaggcgc gcgtgatgga ggcgttcaag cctgcctacg
     4501 gagaagatgc gcgcatttgg tggaggcgct ggcggctctt cttccttgcg accgccggcc
     4561 tctttgggca ccaggcaggc gaggagtggg gcgttagcca ccatctgttg cgccccgtga
     4621 gctgacctcc aacaagcaaa acggcggctc gaaggccgcc gttccactgt gtaatgcctt
     4681 tttctggatt agttcggcat cacgacggtg tcgatgacgt ggattacgcc gttgctcgcg
     4741 ccgatgtcgg ccgtgacaac gttggccgca tcgactttga cgccgtcagt ggcatccacc
     4801 gcaagcgtat cgccctgaac agtcttgact tcggtcgact tgccggcgat gtcactcgac
     4861 atgatcttgc cggggacgac atggtaggtc aggatggcaa ccagcttgtc cttattctcg
     4921 ggcttaagca ggtcctcgac cgtgcctcgc ggcagcttgg cgaacgcctc gtcggtcggg
     4981 gcaaacaccg tgaacggacc gtcgcccttc agcgtatcga ccagaccggc agcctgaacg
     5041 gcagccacca gcgtcttgaa ctggccggcg ccggctgccg tgtcgacgat gtccttggac
     5101 ttggcttgcg ccactgagac cgtgaagacc acgagggcga gagtca
//
LOCUS       DBNHVU010000173        11184 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_508905, whole genome shotgun sequence.
ACCESSION   DBNHVU010000173 DBNHVU010000000
VERSION     DBNHVU010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11184)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11184
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_508905"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /gene="ssrA"
                     /locus_tag="AC16Y7_07025"
     tmRNA           <1..193
                     /gene="ssrA"
                     /locus_tag="AC16Y7_07025"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            247..789
                     /locus_tag="AC16Y7_07030"
     CDS             247..789
                     /locus_tag="AC16Y7_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SspB family protein"
                     /protein_id="HIR5709170.1"
                     /translation="MADEEIRYDLLTQDALRGVVRDVLTQVKRDGLPGEHHLYIAFDT
                     CADGVSVSKRLKEQYPEEMTVVLQYQFWDLEVTDERFEVKLSFSNVPERLVVPFSAVK
                     AFYDPSAQFGLQFGKPGAANDSARQHMASALPDLVGDRPEEANNSDSAPEESGDGDSV
                     EVVMSRPPAEVVQLDSFRKK"
     gene            938..2806
                     /gene="glsA"
                     /locus_tag="AC16Y7_07035"
     CDS             938..2806
                     /gene="glsA"
                     /locus_tag="AC16Y7_07035"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HIR5709171.1"
                     /translation="MDAISDAASARCTALAPIEEYLADLHARASRIEGGAPADYIPEL
                     GKVDPDLLGIAIATVDGQVYGAGDMDVPFTIQSVSKPFMYGYALQHYGRAHVLRQVGV
                     EPTGEAFNSIVLDEVANRPFNPMVNAGAIAIAEMMQGDTQNARVANMLELFSSLAGRK
                     LDIDEDVFASELATGHRNRAIAYMMLNTAMIEHDPSDVLDLYFRQCSLNVTATDLAVM
                     AATLANDGTNPITDQSIYDTQYVRDILTVMNSCGMYDYAGEWAYDVGMPAKSGVSGCI
                     IAVIPGQIGICVYAPPIDKQGNSIRGIRVCHAISNDFQLHAFNNRTNVRTVIRRDYRA
                     NVVRSNRLRTLEERTVLAEKGRKVAVLEAQGALFFGSTEQLLRKMSQLASDSDYVVVD
                     FKRVHLADASACKLIVRAVRAMAQSDTTLVFASITDDGPLDRLTRALAESEEGHNLRR
                     FRDADTAVEWCEDQLLAVAGSDFGTKFSLSQINLFKGLTQDECRLVESVVRPLVFDKG
                     DVIMREGDPAKLFFVLARGTVSVEIKVHGNSERRKRVASIGPGLTFGEMALLDGGTRS
                     ADIVANEKVICYGLGVEELHELSEEHPNVMITILSNLTREFCERLRHANEEISVLD"
     gene            complement(2803..4713)
                     /locus_tag="AC16Y7_07040"
     CDS             complement(2803..4713)
                     /locus_tag="AC16Y7_07040"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5709172.1"
                     /translation="MTAASSHISLPVPRAADASVDVARLAALLVCIIGEVVLAMWLEI
                     IPSDVMPSLVGMTPTAALGVIVAALGLLCLTLRRLRPVARLIGLFLVLMGLLIFSQDL
                     IGYRLGIDEALVPPGDAPKQLTRTAPGVTGSLILFGLGLLYAKRRPTLSLFSQIVALA
                     MLVQVLIVVAGQAYGVMPQDDSFPFMRLSAYGAISAALLAFGLIAASPEHGIASAIIE
                     PSPAGEMLRRLLPGILVVPLIIGWFARQAEVQSLYDSAATLTIFAVANIVVLALFAWA
                     TIGAVRRADGDRQVALVDLRGQREWLSTTLGSIGDGVIACDPNGSVLLLNKIAQELTG
                     WTAAEARGRPIWEVFRVVDEVTRKPVDDPALKALRERAVARLESGALLLTRDGKELPV
                     EHSGAPIVGQDGTLAGAVLVFRDVTERRRTAQRQTMLVGELNHRVKNALAIVQSLVQA
                     SLRQATTKPAQVMAQTLAERLQALHRAHDLLLESQWSGASLKAMVERELDPYRRDGGP
                     KIVIKGPDILLPPQCTSILAMTLHELATNAVKYGALSQNTGQLGVTWKKARGNRLLLA
                     WEERGAPSVPQRRKRTGFGTQLIDKGIRHNLGGETKLEFRQAGLYVELNVPLTPSNEP
                     RAAKPAVQAPAA"
     gene            4930..6351
                     /gene="fumC"
                     /locus_tag="AC16Y7_07045"
     CDS             4930..6351
                     /gene="fumC"
                     /locus_tag="AC16Y7_07045"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIR5709173.1"
                     /translation="MPLADGPQSSAAPRTESDAFGPIKVPGDRYWGAQTERSRQNFKI
                     GDEHMPAPLIRAMALVKKSAALTNLELGRLDKTLAEAIATAADEIVDGKFDGHFPLVV
                     WQTGSGTQTNMNLNEVIANRASEILGGKIGSKSPVHPNDHVNLGQSSNDTFPTAMHIA
                     AAEEIAYRLNPAMSRLFQALKDKAEAFQDVLKIGRTHMQDATPLTLGQEFGGYAAQVS
                     NGIERTKMTLPGLYELAQGGTAVGTGLNSHPRFADLFAAKIADLTKLPFISAENKFEA
                     LAGHDAFVFAHGALATVAASMFKIANDIRLLGSGPRSGFGELLLPENEPGSSIMPGKV
                     NPTQCEAVTMVCAQVMGNQTTVGFAGTQGHLELNVFKPVLAYALLQSIRLLGDVADSF
                     TRNCVAGIEANRERIKEHLERSLMLVTALAPHIGYDKASEIAKQAHKRGTTLREEAVR
                     LGHVSEEDFDRLVKPQEMVAPKE"
     gene            6366..6605
                     /locus_tag="AC16Y7_07050"
     CDS             6366..6605
                     /locus_tag="AC16Y7_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016917686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HIR5709174.1"
                     /translation="MTKARNKRSLTIARHRTSVSLEEPFWTALAALAKQQGKSIAELV
                     GEIDKARSGTSEAESLSAAIRLYVLERAQTKDDAL"
     gene            complement(6602..11164)
                     /locus_tag="AC16Y7_07055"
     CDS             complement(6602..11164)
                     /locus_tag="AC16Y7_07055"
                     /inference="COORDINATES: protein motif:HMM:NF017021.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR5709175.1"
                     /translation="MNSLLLTLTALFILVLSALFAAPLFIDWNDYRPAFEEQAGKLLG
                     RDVKVDGQVHLILLPAPELKFDDVKVADQNGSLERPFLEAKSLEVKLDVGTLLTGAVE
                     AHQLTIVDPTLRLEVNEDGTGNWGDVGRPGVPAPFAPKDVLLDSVQVSGGTVEILKDG
                     VQKFVFDNIDGEASAASLSGPYKVSAEYDFDDRRQRLRFSTGVMDAGQFRLKAALRDP
                     DRSTTYRIDGQVTGLGGKPAYDGSILMRMTDPQAVFAAQKPEAQPGDPAETPTPSGPR
                     DTASFVELKGALKASPDRAELPEFDLTIHANGRPQILKGNLAVEFGEPSKTVGQLAAR
                     WIDLDTLFGTPTDEKRPSPAAVLYMFAEWLLDEAQRSGESKLTVDIEQAGLGGDLVGD
                     LQMKLQSRDGAVTIETLKAVLPGDNRVAVSGKLSQGDFGALFSGPVEVDGSGLRALTR
                     WAAGDRTVSGRTSIGEFSLSAVATVGDGELKLADALGEVSGTKFRGRLNYQAGDKNLI
                     EVNLDSDRMDLREMLGDGPIWSAWLSPDGGNDAAPQSGGGNLLTQLRDDDVRATLQVG
                     ELLLPNIPPGKLDAKLTLAGGTLDVEALDFLAPGAITLNGKGRIENVADAPQGRVDVT
                     LQAETTESVRVLSELFGFSEDVSGSKQLSALVPLDVQAGLVAARNETATKASLELSGK
                     IGGSDIGLNAHATGSPAKLADAEIDIAGSVTGDRPQALLALLFPQLPQDRLSAAAGTE
                     GTLSVEVAGVPSSKLTGKASLVTRAMQLAVEGQGALTDDGVTLNGFGSIATEDASLAI
                     PLFGLDAPPSATGVPLTVSANIAKDGDEIELEQVKARIGGDAVEGNARFERDGAKTTF
                     KIAANADRVSLPAVLGILVAWERSSETEEMLGTISADTAEVWPARGFALDIIDQAQGD
                     VTLNAKTLMLGTPFEVKGATLNARVDDDGIAVTGLDGQLFDGAFAASGTLSPRGNGAA
                     LTAQAKLQGASLEELSQGVAGKKLAEGPFDLGFGVEGEGLSPPGIVAGLSGDGSLSLR
                     AGKLFALESGPLRQIAAKAAKTEIKATKAEIDADTKKIRDTLPKGTYAYAPVNIALEV
                     ENGTLRLVPATLAGKGADTGLTAHVELASLDLDSEWKVKLTGADNKDVPPVTMALAGP
                     LDQTDSITPVIDTAAIESYLTIRRLKEDVERLETLDVTGQSASDAEAKRAAAEKAAAE
                     AKAAAEAKAAAERAAREQAEAEAAQKAKEAEEQAARERAAAEAKAAAEAKAAAEAKAA
                     AEAKAAEEQAARERAEAEAAREAKLAAEKAAAEKAAKERAAAEAQAAAEAKAAAQAKA
                     KAEAEAARKAKVAAEKAAKERAAAEAKAAAEAKAAAEAKAAAQAKAKAEAEATRKAKA
                     AAEKAAKERAAAEAQAAADAKAAAAAKAAAEKSAKERAAAEAKAAADRAARARIEAEA
                     KAAAEANAAATVPSAILPSELPSSDAATDPIAEVLSGEAGPEGVDAVEGETAEEPAPE
                     PARRRYRRKREAPDAWKKNYPIFGGR"
BASE COUNT         1968 a         3691 c         3462 g         2063 t
ORIGIN      
        1 gagggtgtaa aggtcgtact ttcgctcggc atggtatccg ccgttatcgg gccaaaccaa
       61 atagttgcca atgacaacta tgcggaggca cgtctcgctg cgtagtcagc gcgatagcct
      121 aaattaagtc ctagcctcct cgcaggggtt aggcgcggtt tcggagggca ccgggcaaca
      181 gaagccctcc acttgacttg acggggtgtc ctgcctcacg tcgcgtaatc tggagcgacg
      241 ccatctatgg cagacgaaga aattagatac gacctactga cacaggacgc gcttcgcggc
      301 gtggttcgtg acgtcttgac tcaggtcaag cgcgacggtc ttccgggcga acatcacctc
      361 tatatcgcct tcgacacgtg tgcggacggc gtgtccgtct ccaagcgcct gaaggagcag
      421 tatccggagg agatgaccgt tgtcctccag taccagttct gggacctgga agtgacggac
      481 gaacgcttcg aggtgaagct gtccttctcc aacgtgcccg aacgtctcgt ggtgccgttc
      541 agcgcggtga aggcgttcta cgacccgtcg gcgcagttcg ggctccaatt cggcaaaccc
      601 ggcgcggcca acgactcggc gcggcagcat atggcgtccg ccctccccga cctcgtgggc
      661 gaccggcctg aagaggccaa caactcggat tcggctccgg aggagtccgg cgatggggat
      721 agcgtcgaag tggtcatgtc gcggccgccg gccgaagtgg tgcagctcga cagcttccgt
      781 aaaaagtaac tgctctcccc aaggcgtcct gcccaaattt caggcaatgt gttcgagccg
      841 tttcgcggtc aggggcgcat atccagtctt tgggtctggc acggattctg cctaaagtac
      901 cccacgcgac ttggaccttt gggggacgct agacaggatg gacgcgattt ccgacgcggc
      961 ctcggcccgg tgcacagctc ttgctccgat cgaggagtat ctcgccgatc ttcacgcgcg
     1021 ggcgtcgcgg atcgaaggtg gcgcgccggc ggactacatt cccgaactcg gcaaggtcga
     1081 tcctgacctt ttgggcatcg ccatcgccac ggtggacgga caggtctatg gggcgggcga
     1141 catggatgtc cccttcacca tccagtccgt ctccaagccc ttcatgtacg gctacgcgct
     1201 ccagcattac ggccgcgcgc acgtgctccg acaagtcggc gtggagccga ccggcgaggc
     1261 cttcaactcg atcgtgctgg acgaggtcgc caatcgccct ttcaacccga tggtgaatgc
     1321 cggcgcgatc gccattgccg agatgatgca gggcgacacg cagaatgcgc gcgtcgcgaa
     1381 catgctcgag ttgttctcaa gcctcgcggg acgcaagctg gacatcgacg aggacgtttt
     1441 tgcttcggag ctcgccaccg ggcaccgcaa tcgtgccatc gcctacatga tgctcaacac
     1501 ggcgatgatt gaacatgacc cgagcgacgt cctcgatctg tactttcgcc aatgctcgct
     1561 caacgttacc gccaccgacc ttgccgtcat ggccgcgaca ctcgcgaatg acggcacaaa
     1621 tcccatcacc gatcagagta tctacgacac ccaatatgtg cgcgatatcc tgaccgtgat
     1681 gaactcctgc ggcatgtacg actatgccgg cgagtgggcc tacgatgtcg gcatgcccgc
     1741 caagagcggc gtttcaggct gcatcatcgc ggtcatccca ggccagatcg gcatttgtgt
     1801 ctacgcgcca cccatagaca agcagggcaa cagcattcgc ggcatccgcg tatgccacgc
     1861 gatttccaac gatttccaac ttcacgcctt caacaatcgc accaatgtgc gcaccgtcat
     1921 aaggcgtgat taccgggcaa acgtcgtccg ctcaaaccgc ctgcgcacac ttgaggagcg
     1981 cacggtcctc gccgagaaag gccggaaggt cgcagtcctc gaggcgcaag gcgcgctgtt
     2041 ctttggctct accgagcagc tgcttcgcaa aatgtctcag ctggcctcgg actccgacta
     2101 tgtagtcgtt gacttcaagc gcgttcatct cgccgatgcg tctgcgtgca agctcatcgt
     2161 gcgtgccgtg cgcgccatgg cgcagagcga tacgaccctg gtttttgcgt cgatcaccga
     2221 tgatgggccg ctggaccggc tgacgcgcgc gctggccgaa agcgaggaag gtcacaatct
     2281 gcgacggttc cgcgacgcag acactgcagt cgaatggtgc gaggaccagc tcttggcagt
     2341 cgcgggttcg gacttcggca ccaagttctc gctctcgcag atcaacctgt tcaaggggct
     2401 cacccaggac gaatgccgtc tcgtggaatc cgtcgtaagg ccacttgtct tcgacaaggg
     2461 agacgtgatc atgcgtgaag gcgatcccgc caagctattc ttcgtcctgg cacgcggcac
     2521 ggtgagtgtt gagatcaagg ttcatggcaa cagcgaacgg cgtaagcgcg tggcatcgat
     2581 cgggcccggg ctcacattcg gggagatggc gctgctcgac ggcggaacgc gctccgcgga
     2641 tatcgtcgcc aatgagaagg tgatttgcta cggcctcggg gtcgaggaac ttcacgagct
     2701 gtccgaggag cacccgaacg tcatgatcac aattctgagc aatttgacgc gggaattctg
     2761 cgaacgcctg cgccatgcca atgaggaaat cagcgtcctc gactaggccg ccggcgcctg
     2821 cacggccggc ttcgccgccc gcggttcgtt cgatggcgtg agcggcacgt tcaattccac
     2881 ataaagcccc gcctgcctaa actcgagctt ggtttctcca ccaagattgt ggcgaatgcc
     2941 cttgtcgatg agctgcgtgc cgaagccagt ccgcttgcgc ctctgcggaa cggacggcgc
     3001 gccgcgttct tcccaggcaa ggagaagccg gttgccgcgg gccttcttcc aggtcacgcc
     3061 gagctgaccg gtgttctgcg atagcgcgcc atacttcacg gcgttggtgg caagctcgtg
     3121 gagcgtcatg gcgaggatcg acgtgcattg cggcggcagc aatatgtccg gccccttgat
     3181 gacgatcttc ggtccgccgt cgcggcggta aggatcgagc tcccgttcga ccatcgcctt
     3241 gaggctcgcg cccgaccact gggactcgag cagaagatcg tgcgcccgat gcaaggcctg
     3301 caaccgctcg gcaagcgtct gcgccatcac ttgcgccggt ttggtcgtag cttggcgcaa
     3361 gcttgcctgc accaaggact gcacgatggc gagcgcgttc ttgacgcgat ggttgagctc
     3421 gccgaccagc atggtctgcc gctgcgcggt gcgccggcgc tcggtcacgt cgcggaacac
     3481 gagcacggcg ccggccaacg tcccgtcttg cccgacgatg ggcgcaccgc tgtgctccac
     3541 cggcagttcc ttgccgtcac gcgtgagcag aagcgcgcct gactccagtc tcgcgaccgc
     3601 gcgctcgcgc agcgctttga gcgccgggtc gtcgacaggc ttgcgcgtga cttcgtcaac
     3661 cacccggaag acctcccaga tggggcgccc gcgcgcttcc gccgccgtcc acccggtcaa
     3721 ctcctgagcg atcttgttga gcagaagcac gctgccgttc ggatcgcagg cgatgacgcc
     3781 gtcgccaatc gatccgagcg tcgtggacag ccattcgcgt tggccgcgca aatccaccag
     3841 cgccacttgg cgatcgccgt cggcccgccg gacagctccg atcgtggccc aggcgaacag
     3901 agcgagcaca acgatgttcg ccaccgcgaa gatggtgagc gtcgccgcac tgtcgtagag
     3961 cgactgcacc tcggcttggc gcgcgaacca gccaatgatc aacggcacca cgagaatgcc
     4021 gggcaagagg cggcgcagca tctcgcccgc aggcgacggc tcgatgatcg cggaggcaat
     4081 gccgtgctcc ggcgaggcgg cgatcaaacc gaaagcgagc agcgccgcgc tgatcgcgcc
     4141 ataggccgac agcctcatga acgggaagga gtcgtcctgc ggcatgacgc cataggcttg
     4201 gcctgcgacc acgatgagga cctgcaccag catggccagg gcgacgatct gactgaacag
     4261 agacagcgtc ggccggcgct tggcatagag gagcccaagc ccgaagagga tcagcgagcc
     4321 cgtgacgccc ggcgccgtgc gggtcaattg ctttggggcg tcgccgggtg gcacaagggc
     4381 ttcgtctatc cccagccggt agccgatcaa gtcctggctg aagatgagca ggcccatgag
     4441 gacgagaaac aggccgatga gacgggcgac cggccgcaag cgccgaagcg tgagacacaa
     4501 cagtccgagg gcagcgacaa tcacgccgag cgcggccgtg ggcgtcatcc cgacaaggct
     4561 gggcatgaca tcgctcggga tgatctccag ccacatggcc agaaccacct cgccgatgat
     4621 gcacacgaga agcgcagcaa gccgcgcaac gtccaccgat gcgtcggccg cgcgcggaac
     4681 cggcaatgaa atatgactcg atgctgccgt cactcgcctt cctcgcctgg cctcccagca
     4741 acgccccccg caggcagctt ggacgctccg aggacaaaag ccccaaaaaa gatcgcgacg
     4801 agtccggttc tctcaccctc tcgcgcgatg ttatagccta ggccccagac ccccgatagc
     4861 caatcggcga ccgccgccag gcacacgggc tgccaggata ccaagactgc caaaaaggga
     4921 attgcgacca tgcctctagc tgacggcccc cagtcctccg ccgcacctcg tacagaatcg
     4981 gacgctttcg gcccgatcaa ggtcccgggc gaccgctatt ggggcgccca gaccgaacgc
     5041 tcgcggcaaa acttcaagat cggcgatgag cacatgcccg cccccctgat ccgcgccatg
     5101 gccctggtga aaaagtcggc ggcactgacc aatctggagc tcgggcgtct cgacaagacc
     5161 ctggccgagg cgatcgccac ggcggcagac gagatcgtgg acgggaagtt cgatggccat
     5221 tttcctttgg tggtttggca gaccgggtcc ggcacccaga ccaatatgaa cctgaacgag
     5281 gtgatcgcga accgcgccag cgagatcttg ggcggaaaga tcggcagcaa gtcgccggtt
     5341 caccccaacg atcacgtcaa ccttggccag tcctcgaacg atacatttcc gacggcaatg
     5401 cacatcgcgg cggccgagga gatcgcttac cggctcaatc ccgcgatgtc ccgcctcttc
     5461 caggccctga aagacaaggc cgaggcgttc caggacgtgc tcaagattgg gcgcacccat
     5521 atgcaggacg cgaccccctt gaccctgggg caagaattcg gcggctatgc ggcgcaggtt
     5581 agcaacggca tcgagcggac gaagatgacg ctgcccgggc tgtacgaact cgcccagggc
     5641 ggcaccgccg tgggcaccgg gctcaattca catccgcgct ttgccgatct cttcgcggcc
     5701 aagatcgcgg atctgacgaa gctccccttc atcagcgcgg agaacaaatt cgaggcgctg
     5761 gccgggcatg acgccttcgt cttcgcccat ggcgcgctgg ccacggtggc ggcgtcgatg
     5821 ttcaagatcg ccaacgacat tcgcctgctc ggctccggac cgcgctccgg cttcggcgaa
     5881 ctcttgcttc cggagaacga gcccggctcg tcgatcatgc cgggcaaggt gaaccccacg
     5941 cagtgcgagg ccgtgaccat ggtctgcgcg caagtcatgg gcaaccagac aacggtcggc
     6001 tttgccggaa cccaggggca tttggaactc aacgtgttca agccggtgct ggcctacgcg
     6061 cttctccaat cgatccggct cttgggcgac gtggccgaca gcttcaccag aaactgcgtt
     6121 gcagggatcg aagccaatcg tgagcggatc aaggaacact tggaacgctc attgatgctg
     6181 gtcaccgcgc tcgccccgca tatcggctac gacaaggcga gcgagatcgc caagcaggcg
     6241 cataaacgcg gcacaacgct ccgcgaggaa gcggtgcggc tcggccatgt gagtgaggag
     6301 gacttcgacc gcctggtgaa gcctcaggag atggtcgcgc ccaaggagtg acgatcacgc
     6361 cgttgatgac aaaggcccgc aacaagcgct cgctgacgat cgccagacac cgcaccagcg
     6421 tgtccttgga ggagccgttt tggaccgcgc tcgcagcgct tgccaagcaa cagggcaaat
     6481 caatcgcgga gcttgtcggc gaaatcgaca aagcgcgtag cgggacaagc gaagcggaga
     6541 gcctttccgc cgcgatcaga ctctatgtcc tggaacgcgc gcagacgaaa gacgatgcgc
     6601 tctagcggcc accgaaaatg gggtagttct tcttccaggc gtcgggcgct tccctcttgc
     6661 ggcggtagcg ccgtcgcgcg ggctctggcg caggctcttc ggctgtctcc ccttccaccg
     6721 cgtccacgcc ctctggacct gcttcgcctg agagaacctc cgcgatcgga tccgttgccg
     6781 catcgctgga cggcaattcg gacggcaaaa tcgcgctcgg caccgttgcc gcagcgttgg
     6841 cttccgcagc ggccttagcc tccgcttcga tgcgcgcgcg ggcggcacgg tccgcagcag
     6901 cttttgcctc cgcagcggca cgctctttcg ctgacttctc cgccgcagcc tttgccgcgg
     6961 cggcggcttt ggcatcggcg gcagcttgtg cctctgccgc ggcccgctcc ttggcggcct
     7021 tctccgccgc agctttggct ttccgtgtgg cttctgcttc agctttcgcc tttgcctgcg
     7081 cggcggcctt ggcttctgca gccgctttgg cttccgctgc ggcttttgcc tcagcggcgg
     7141 cgcgctcctt ggccgccttc tccgccgcaa ccttcgcttt tcgtgcagcc tctgcttcag
     7201 cctttgcctt tgcctgtgcg gcggctttgg cttccgctgc agcttgtgcc tcggcggcgg
     7261 cgcgctcctt ggctgccttc tctgctgccg ccttctctgc ggccaatttg gcttctcgtg
     7321 cggcttcggc ctcggcccgt tcgcgcgcag cctgctcttc cgcggctttt gcctcggcgg
     7381 cagctttcgc ttcggcagca gcctttgctt cggcggcggc tttggcttct gcggcagccc
     7441 gttcccgggc cgcctgttcc tcggcctctt tcgccttttg tgcagcttcg gcttcggcct
     7501 gttcgcgcgc ggcgcgctcc gctgcggcct ttgcttcggc ggcggctttg gcttcggccg
     7561 cggccttttc cgccgcagcg cgcttcgcct cggcatcgga cgcagactgc cccgtcacgt
     7621 ccagcgtctc caagcgctcg acatcctctt tcagccgccg gatcgtcaga tagctctcga
     7681 tggccgccgt atcgatcaca ggcgtgatgc tgtccgtttg atcgagcgga ccggcgagcg
     7741 ccatggtcac cggcggcaca tccttgttgt ccgcgccggt cagtttcact ttccactcac
     7801 tgtcgagatc gagacttgca agctccacat gagcggtgag gccggtgtca gcgcccttcc
     7861 ctgcaagcgt cgcaggcaca aggcgtaagg tgccgttctc cacctctagc gcaatgttca
     7921 caggtgcgta cgcgtacgtt ccttttggaa gcgtgtcgcg gatcttcttg gtgtccgcgt
     7981 cgatctcggc cttggtggct ttgatttcgg tcttcgccgc tttggctgca atttggcgca
     8041 agggccccga ctcaagcgcg aagagcttgc ccgctctaag ggacagactg ccgtctccgc
     8101 tcaacccggc gacgatgccg ggcgggctga ggccctcccc ttcgacgcca aagccgagat
     8161 cgaacggacc ttcggcgagt ttcttgccgg caacgccttg cgacagctct tcgaggctag
     8221 cgccttgcag tttggcttgc gccgtgagcg ccgcgccgtt gccgcgcggc gacaacgtgc
     8281 cggaggcggc aaaagcgcca tcgaatagct ggccatccaa gccggtgacg gcaatcccat
     8341 cgtcatcgac gcgcgcattg agcgtggcgc ccttgacctc gaagggcgtg ccaagcatga
     8401 gcgtcttggc gttcagcgtg acgtcgcctt gcgcctggtc gatgatgtcg agcgcgaacc
     8461 cgcgcgcagg ccagacctcc gccgtgtcgg cgctgatggt gccgagcatc tcttcggtct
     8521 ccgacgagcg ctcccaggcc acgaggatcc cgagcaccgc cggaagcgac acacggtctg
     8581 cgttcgcggc aattttgaat gtcgtcttgg ccccgtcgcg ctcgaaccgc gcgttgcctt
     8641 caacggcgtc tccgccgatt ctggccttga cctgctcgag ctcgatctcg tccccgtcct
     8701 tggcgatgtt ggcgctcacg gtgagcggca cacccgtcgc gctgggcggt gcatcgagac
     8761 caaagagcgg aatggccagg ctcgcatctt ccgtggcaat cgaaccgaag ccattcagcg
     8821 tcacgccatc gtccgtcagc gcgccttggc cctcgacggc cagttgcatg gcgcgggtta
     8881 cgagcgacgc cttgccggtg agcttactgc tcggcacgcc cgccacttcc acggacagcg
     8941 tgccttccgt gccggcggcc gccgaaagcc tgtcttgggg caattgcgga aacaacagcg
     9001 caagcaaggc ttgcggccgg tcacctgtga cactgccggc gatatcgatt tccgcgtccg
     9061 cgagcttcgc cgggcttccc gtggcgtgcg cgttaagacc gatgtcggag ccgccgatct
     9121 tgccgctcaa ctccagcgaa gccttggtcg ccgtctcgtt gcgcgcggcc acgagaccgg
     9181 cttgcacatc gagcggcacc agcgccgaga gctgcttaga gccgctgaca tcctcggaga
     9241 atccgaatag ctcggacagg acgcgcacgc tttcggttgt ctcagcctgc aacgtcacgt
     9301 ccacgcgtcc ttgcggggcg tctgccacgt tttcgatcct gcccttgccg ttgagggtga
     9361 tggcgccggg ggccagaaaa tccaaagcct ccacgtccag ggtcccgcca gccagggtca
     9421 gcttcgcgtc gagcttgccg ggtggaatat tcggcagaag gagttcgccg acctgaagcg
     9481 tggcgcgcac gtcatcgtcg cgcagctgcg tcagaaggtt accgcctccg ctttgcggcg
     9541 cggcatcatt gccgccgtcc ggcgacagcc aggcgctcca aattggaccg tcgcccagca
     9601 tctcccgcag atccatgcga tcgctgtcga ggttcacttc gattaggttt ttgtcgcccg
     9661 cctggtagtt gagccggcca cggaacttcg tgccgctcac ttcgccaagc gcgtcggcaa
     9721 gcttgagctc cccatcgccg acggtggcga cagccgagag cgaaaactct ccaatagagg
     9781 tgcgccccga cacggtgcga tcgccggccg cccaccgtgt cagtgcgcgc aagcccgacc
     9841 cgtcgacctc gaccggccct gagaagagcg caccaaaatc accttggctg agcttgccgg
     9901 agaccgcgac gcggttgtcg ccgggcagga ccgccttcag cgtctcgatg gtgacggcgc
     9961 catcgcggct ctgcagcttc atttgaagat cgccaacgag gtcgccgcca agcccggcct
    10021 gctcgatgtc gacggtgagc ttgctttcgc cgctgcgctg ggcttcgtcc aagagccact
    10081 cggcaaacat gtacagcacg gcagcgggcg atggccgctt ctcgtcggtc ggagtgccga
    10141 acagagtatc gagatcgatc caacgggcgg cgagttggcc taccgtcttg gacggctcac
    10201 cgaactcgac ggcgagattg cccttcagga tctgcgggcg gccattggcg tgaatggtga
    10261 gatcgaactc tggcaactcg gccctgtccg gcgacgcttt taatgcgcct ttcagttcga
    10321 cgaaggacgc cgtatcgcgc ggaccgcttg gcgtgggcgt ctcagccgga tcaccgggtt
    10381 gcgcctcggg cttctgcgcc gcgaaaacgg cctgcgggtc ggtcatccgc atcaaaatgc
    10441 tgccgtcata ggccggcttg ccgccaagcc ccgtcacttg gccgtcgatc cggtaggtcg
    10501 tcgaacggtc gggatcgcgc aaggccgcct tgaggcggaa ctgaccggcg tccatgaccc
    10561 ccgtcgaaaa ccgcaaacgc tggcggcggt cgtcgaagtc gtattcggcg gacaccttgt
    10621 acgggcccga caaggacgcg gcgctggctt cgccgtcgat attgtcgaag acgaatttct
    10681 gaacgccgtc cttgagaatc tcgacggtgc cgccgctgac ctgcacggag tcgagcagca
    10741 catccttagg cgcgaatggc gcgggcacgc cgggcctgcc gacatcgccc caattgccgg
    10801 ttccgtcctc gttgacctca agccgcaagg tcggatcgac aatggtgagt tggtgcgcct
    10861 cgacggcgcc cgtcaggagc gtaccgacat cgagcttcac ctccagcgac ttggcctcca
    10921 ggaacgggcg ctccaagctg ccgttctggt ccgccacctt gacgtcgtcg aatttgagct
    10981 cgggcgctgg aagcaggatg agatgcacct ggccgtcgac cttgacgtca cggccaagca
    11041 gcttgcccgc ctgttcttca aaggccggcc ggtagtcgtt ccaatcgatg aagagcggcg
    11101 cagcgaagag cgcgctcagc accagaatga acaacgccgt gagtgtgagc agaagcgagt
    11161 tcaccgatgt ctctgcctaa ggct
//
LOCUS       DBNHVU010000174        21676 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_881314, whole genome shotgun sequence.
ACCESSION   DBNHVU010000174 DBNHVU010000000
VERSION     DBNHVU010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 21676)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21676)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21676
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_881314"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /gene="rplT"
                     /locus_tag="AC16Y7_07060"
     CDS             complement(<1..324)
                     /gene="rplT"
                     /locus_tag="AC16Y7_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008190886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR5709176.1"
                     /translation="MSRVKRGVTAHARHRKVLTKAKGYYGRRKNTIRVAKQAVEKAGQ
                     YAYRDRKVKKRTFRSLWIQRINAAARANGTTYGQLIDGLKKAGIELDRKVLADIAVRE
                     PQTFQG"
     gene            complement(359..559)
                     /gene="rpmI"
                     /locus_tag="AC16Y7_07065"
     CDS             complement(359..559)
                     /gene="rpmI"
                     /locus_tag="AC16Y7_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR5709177.1"
                     /translation="MPKLKTKSGAKKRFKVTATGKVKCAAAGKQHGMIKRTNKFIRNA
                     RGTMVLADENAKIVKKSFLPYA"
     gene            complement(737..1264)
                     /gene="infC"
                     /locus_tag="AC16Y7_07070"
     CDS             complement(737..1264)
                     /gene="infC"
                     /locus_tag="AC16Y7_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR5709178.1"
                     /translation="MKLAPNRGGPRVNEGIDTPEIQLIDATGENVGVVGIEDALAQAA
                     DAGLDLVEISPNSKPPVCKILDFGKYKYQAQKKAAEARKKQRTVEIKEIKMRPNIDTH
                     DYDVKMRSMNRFFEEGDKVKVTLRFRGREMAHQDLGVQLLNKVKDDTAEIAKVESEPR
                     LEGRQMVMVLAPIRA"
     gene            complement(1465..2322)
                     /locus_tag="AC16Y7_07075"
     CDS             complement(1465..2322)
                     /locus_tag="AC16Y7_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709179.1"
                     /translation="MYHVPKCAGRTIDRHLAQSLPQSAYHRLRRRRGLRRFASRYDGA
                     DITSPNDIRVLSGHYLGISVDPLFERRLIKRSLLLRDPASHFVSYYNYRMTRYISQGL
                     QPYGVDVAYSAMQRNFITHYILKNFLELTWTQIASLSDDEKYDLANAFLSTFWFVGDY
                     QLCDEFVGVLGEQLGISANARPHNTLTELARGAGWTPLTLELLPRDTVSNIVSENMID
                     QKLWETWHDARHETCFVRPVALENSTSSFVSGEAVRLVSQIMRRAQRRWGTFDAPVPV
                     MHGDPATPA"
     gene            complement(2540..3721)
                     /locus_tag="AC16Y7_07080"
     CDS             complement(2540..3721)
                     /locus_tag="AC16Y7_07080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020083992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5709180.1"
                     /translation="MSEVTILQVVPRLEAGGSEQSTIEIAEALTAAGAKALVASEGGR
                     LVERLENAGGELIRLPLASKNPLTILANAQRLAELVEARDVSLLHARSRAPAWSAFLA
                     ARRTGRPFVTTYHGAYRRQFPLKQAYNGVMARGDRVIANSRYTAEIIAARHGVPMERM
                     RVIHRGLDIAQFARDAIAPARFQRLRDAWGIEEGCPVILQAARLTRLKGHTHTIEAAR
                     LLREAGQLGDAVVIFAGSADGRDGYREELEKKIADGGLEGRVRLVGHCDDMPAAFNLA
                     TVGILPSTRAETFGRPSIEAQAAGCPVIVTDVGAAPENVIAASDSSDFTGWVVPPSDA
                     PALAESLAEALALPPDARATICQRARAHVGCHFALKTMQRDTLAVYDELLGTNLASHL
                     R"
     gene            3757..4593
                     /locus_tag="AC16Y7_07085"
     CDS             3757..4593
                     /locus_tag="AC16Y7_07085"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5709181.1"
                     /translation="MDTLALVSMQHEPQFVEIGQDSKRQASKRRRIAYRFDEGQDASS
                     PAVFWLSGFLSDMASTKVSALSAWAKHEGHAMLCFDYSGHGVSGGDILKASVGDWLEE
                     AATMLELLKGRRVILVGSSLGGWIGLLLARALAKSGDARLGGLVLIAPAWDMTEKLMW
                     EKMSEKTRVIVETEGVFYAPSDYDDAYPLTKVLIEEGRNHLLAAGGIEVGCPVRILQG
                     LRDEDVPWQHALALVDLIEGDDVEITLVKDGNHRLSSPEDLRRLTRTVGALIETAASP
                     SA"
     gene            complement(4562..5443)
                     /locus_tag="AC16Y7_07090"
     CDS             complement(4562..5443)
                     /locus_tag="AC16Y7_07090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5709182.1"
                     /translation="MSRLFAFGLGFSARALAPLLASQGWEIAGTAREEPKIGQLRREG
                     FEATRFAGDKSNSELPKLLQGTTHLLHSVPPGDEGDPVLASYRDEIAKLKTLEWIGYL
                     STVGVYGDQQGNWVSEETVPKPNSARTEARVVAEQDWLDFGEKIGVPVQVFRLAGIYG
                     PGRSVFDKLKAGTARRINKDGQVFSRVHVDDIARVLEASIAKPRAGAIYNVADDEPAA
                     PGDVVAHVAQMIGVAPPPEIAFEDADLTPLARSFYQGSRKIGNALIKSELGVELRYPT
                     FREGLRSLMPKETPPSR"
     gene            complement(5440..6693)
                     /gene="queG"
                     /locus_tag="AC16Y7_07095"
     CDS             complement(5440..6693)
                     /gene="queG"
                     /locus_tag="AC16Y7_07095"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIR5709183.1"
                     /translation="MRWSSRGHLSGRCCRTAWPASRLQRHTRISTSEAAPQIMRVWQA
                     GMTATLRSTIAEEAKSLGFDAVRFTSANVPASIGDALDRFIAEGRQGDMAWLAETATR
                     RKSPRALWPDAQSVIVLAVNYGVEGDPLAALRKRSHGAVSIYAKRADYHDVIKKNLKV
                     LAGRVQALTQGGVKVFVDTAPVMEKPLAARAGIGWQGKHTNLVSREFGSWLFLGSIFT
                     TAEIAPDEPEEDHCGECRRCLDVCPTNAFTAPYELDARACISYLTIEHKGHIAPQYRA
                     AMGNRIYGCDDCLAVCPWNKFAQVSHDAKLTVRASADNPPLNALLMLDDAGFRKRFRG
                     TPIKRTGRNRFLRNVLIAAGNSGDTALAPKVEALLSDESPLVRAMAVWALSRLAPETF
                     HSLRATFAGQESDSDVRGEWLEVAA"
     gene            complement(6596..7282)
                     /locus_tag="AC16Y7_07100"
     CDS             complement(6596..7282)
                     /locus_tag="AC16Y7_07100"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HIR5709184.1"
                     /translation="MANLHHHPLCAFSRSIRLALAECGLEVALTEEKPWEWRQELLAL
                     NPAGTLPVFVPDEGAPIAGSYAISEYLGDTAEARDPRGFQPFPGGLAERAEARRLVDW
                     IHHKFHDEVTDYLVVEKVFRRFGPQSASPDMEAMRAGHDNLRYHLTYVGHLTETRSWL
                     AGDVMSFADLAAAAHLSALDYLGEVPWEEFEPAKNWYALVKSRPSFRPLLQDRVAGFT
                     PSETYTNLDF"
     gene            7383..8180
                     /locus_tag="AC16Y7_07105"
     CDS             7383..8180
                     /locus_tag="AC16Y7_07105"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009464911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR5709185.1"
                     /translation="MTVEQIIVLAVVQGITEFLPISSSGHLILVPYLLHWADQGQFVD
                     VMVHVGTLFAILIYFWRDVWQITLGGLELFKGKVTHEGKLAIYIVLATIPAVAFGLFL
                     KKFGFGDLERSVTVVAWNTVLYGLLMLVADMVGPQEKTIENMTLRSALIIGVAQALAL
                     IPGTSRSGVTMTAARFLGFTRPDAARFSFLLGIPAIAGAGVLTVGEALAAGDTITMDA
                     VYCAILTFFAGLAAIAFLMALVKRISFLPFVLYRMVLGGFLLVMIYT"
     gene            complement(8194..9162)
                     /locus_tag="AC16Y7_07110"
     CDS             complement(8194..9162)
                     /locus_tag="AC16Y7_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HIR5709186.1"
                     /translation="MALNNRALVTVYGGSGFLGRHTVRAIAQTGARIRVAVRRPELAG
                     HLQPLGGVGQIVPVQAKVRFPDSLLAAAQDADAVVNLVGILLPTGKQTFKSVQDEGAR
                     NVAEAAKAAGVGTLVHVSAIGADASSASIYARSKAAGEAAIKDVYKDATIFRPSVVFG
                     PEDEFFNRFAALARLSPMLPLIGGGHTKFQPVFVGNVAQSIVAALEGRTSTKAPYELG
                     GAEILTMRQVMERVLAYSMRSRPLVPLPFWIAKLQGAVLQWLPNPPLTLDQVRLLETD
                     NVVSAAAIKAKRTLDGLGIEPVQIANVVPDYLEQFRPRGQFSIYKA"
     gene            9295..9381
                     /locus_tag="AC16Y7_07115"
     tRNA            9295..9381
                     /locus_tag="AC16Y7_07115"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9329..9331,aa:Leu,seq:cag)
     gene            9411..10058
                     /locus_tag="AC16Y7_07120"
     CDS             9411..10058
                     /locus_tag="AC16Y7_07120"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HIR5709187.1"
                     /translation="MPAANVEKPLSQLKNVELYQGDLPEGRFTAKTIAIDTETLGLNP
                     HRDRLCLVQLSDGDGTAALVQVKAGQDAPELKRLLADESVLKLFHFARFDLAALNNFF
                     GVMAGPVYCTKIASKLARTYTDRHGLKDLVRELLGVDLSKQQQSSDWGAAELSPEQLN
                     YAASDVLYLHALRDRLDLMLKREGRTHLAKACFEFLPTRAELDLEGWAEMDIFAH"
     gene            10163..10936
                     /gene="lptC"
                     /locus_tag="AC16Y7_07125"
     CDS             10163..10936
                     /gene="lptC"
                     /locus_tag="AC16Y7_07125"
                     /inference="COORDINATES: protein motif:HMM:NF018537.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HIR5709188.1"
                     /translation="MAYEANLGTRGRHYVVRTPEERQRAFRSARRHSFFVSFLRRVLP
                     VLALLVLAAYFISTRMNITVGDFTASIDGMEVVDGNLRMLNPTLEGADKKNGKYVVRA
                     EYADQDITSPDIIKLHAIRADIDDPTGGWSKMQAVRGVFNNTAERLIMKDRITVATSS
                     GVTGELKYATMDMATQTLRSHSPVFFELNNGNATVRANAMTLRSKEKVITFRGKVAVH
                     LEPKKVAAAKKPAQQPVPAANGAAQSGAAPTGAAQVPPQ"
     gene            10933..11691
                     /locus_tag="AC16Y7_07130"
     CDS             10933..11691
                     /locus_tag="AC16Y7_07130"
                     /inference="COORDINATES: protein motif:HMM:NF015901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HIR5709189.1"
                     /translation="MSGCGAIRQALHVLGLGVVLLLPVSPLTAQTMTNSFGGMSKNSD
                     APIDIQSDLLTVYDTKKIAIFKGNVKAVQGTTTLRATQLDVHYIGGAEELTGKGADQD
                     NATTPVAQQGATQKDADPQSSINKLEARGDVYITSEDDQTTSSDWAVYDVPAQLVTVG
                     GNVVLSQGQNVLKGNRLIIDLKTGESRFDNTGNAAAGGGRIRALFMPKGSDVKDSAAA
                     MEPEPGQAQQVPGAQVPGAAVETGEPLPIAPPRR"
     gene            11766..12587
                     /gene="lptB"
                     /locus_tag="AC16Y7_07135"
     CDS             11766..12587
                     /gene="lptB"
                     /locus_tag="AC16Y7_07135"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HIR5709190.1"
                     /translation="MLSFPGHQGTAAQGAPAQGAPLAHGQAPLREPGNGWLTIRSVVK
                     SFKKRMVVRGVSLDLARGEAVGLLGPNGAGKTTVFYMITGLIPADEGSIKIDGRDVTR
                     LPMYRRARLGIGYLPQEASIFRGLTVEENILAVLEMVEPKRKERKKQLDALLEEFRIT
                     RLRKSPSMALSGGERRRVEIARALASRPSFMLLDEPFAGIDPIAVGDIRALVRHLTER
                     GIGVLITDHNVRETLDLVSRAYIIHDGMVLTHGTTDDIINNEDVRRVYLGDQFKI"
     gene            12614..14167
                     /gene="rpoN"
                     /locus_tag="AC16Y7_07140"
     CDS             12614..14167
                     /gene="rpoN"
                     /locus_tag="AC16Y7_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR5709191.1"
                     /translation="MAINTKLELRQAHGLVMTPQLQQAIKLLQLSNMELATFVEGELE
                     RNPLLEQADADEPSNTADQPSPAAKSEEGSSEATGAESGTSDEGDWVNLETGNDSGRT
                     DDLDAEPQDVFPDAENFTPGAVSDSGWSGLTQGPRPDIDQGNNLEAYVAEEKTLRDHL
                     AEQLSLAFSDPARRLIGQHIIDMTDEAGYLRGELDALAELLGAPPELIEETLKVMQSF
                     EPCGVFARDLRECLTLQLKEQDRCDPAMAGLIDNLHLLATHDLVALKRAAHVSDEDLC
                     DMIAEVRRLNPKPGLKYGSSPTQPIVPDVLVRSLPDGSYYVELNSDTLPRVLVNQSYY
                     ATVSKSALRKEDKSYLVDCLQTANWLVKSLDQRARTILKVSQEIVRQQDAFFTYGVRH
                     LRPLNLKTVADAISMHESTVSRVTANKYMATNRGLFELKYFFTSAIPATSEGGTHSSE
                     AVRHRIREMVDAEPAAEVLSDDKIVERLKGDGIDIARRTVAKYREALGIPSSVQRRRL
                     KRIAMGAGA"
     gene            14630..15097
                     /gene="ptsN"
                     /locus_tag="AC16Y7_07145"
     CDS             14630..15097
                     /gene="ptsN"
                     /locus_tag="AC16Y7_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011974228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HIR5709192.1"
                     /translation="MHLGDFISPEAVMASLKVKNKKQLLTELAARVAPMAGVPEREIF
                     DVLLQRERLGSTGLGNGVAIPHGKMAGLPQIVGLFAHLAEPIDFESVDGEPVDIVFLL
                     LAPEGAGADHLKALARISRLLRDEAAVKKLRASEDAAALYALLTEGEAASHAA"
     gene            complement(15094..16230)
                     /gene="ampH"
                     /locus_tag="AC16Y7_07150"
     CDS             complement(15094..16230)
                     /gene="ampH"
                     /locus_tag="AC16Y7_07150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HIR5709193.1"
                     /translation="MRCAIFALLVTASSFLGVSTQAHADALLDEVVTFTGDVLYLQTK
                     VPGLVIGAVRGGDVAIHGVGERSDGGDAPDGDTVLRIGSITKAFTGQVLAALAADGTV
                     SLTQTVGKTAPDLGRGADPNVAKLRLIDIATQSSGLPREVPHEHGPPDDPFAPITRDA
                     FAEWLKTETLLFPPGTGVLYSNFAFDLLSIALSEAAKEPYPDLLKKHVTSPLGMDDTS
                     FVLTDAQRKRFMQGHGFDGKALPNVPSGDVILGSGGLYSTPKDLLRWMQWHLDRLSPE
                     DAEVRMLDHAAYLVRDGLDPVSGMDESGHMDAMSLGWVVMMPEGNRPLILQKAGGLQG
                     TFTYIAFAPTRGVAAFVAINKFDFAAAMGMAEAVNEMIATLAPR"
     gene            complement(16344..16607)
                     /locus_tag="AC16Y7_07155"
     CDS             complement(16344..16607)
                     /locus_tag="AC16Y7_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BQ00720 family protein"
                     /protein_id="HIR5709194.1"
                     /translation="MDKTENNETRPMSLKELALLGDGEVAYIKQVDTDAAEQLFPTLD
                     EALEGIDLFAVLSADGTPIALADSRTAAIANAIENDLVPVSVH"
     gene            complement(16667..17080)
                     /locus_tag="AC16Y7_07160"
     CDS             complement(16667..17080)
                     /locus_tag="AC16Y7_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HIR5709195.1"
                     /translation="MALLDGNLWLGLSTGRLDARAKTSEDGYPPYNVELLHDSDCGQE
                     ALRITLAVAGFGRDELEVSIENGELAIRGAKREEGPKDYLHRGIAARKFERTFALAKG
                     VEVRKAELHDGLLAIELERPHREKRVLKVWITPAG"
     gene            17258..17506
                     /locus_tag="AC16Y7_07165"
     CDS             17258..17506
                     /locus_tag="AC16Y7_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HIR5709196.1"
                     /translation="MMPQQPNWMLSAMTFFVNPNTGNHSNDGRSEKTAFRNLSHALDA
                     AAAGDKILVTPATYDEDLPKLIARAKAAGITVAVLGGH"
     gene            17611..18546
                     /locus_tag="AC16Y7_07170"
     CDS             17611..18546
                     /locus_tag="AC16Y7_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein relay system zinc metallohydrolase
                     2"
                     /protein_id="HIR5709197.1"
                     /translation="MSRRNLFGAALALAAAPLLRYDPAQAASVDPSEVAPGIFVHQGR
                     YEVQSPDNRGDIANASFVVGSDGVAVIDTSGSAKMGAELREAIRAVTDKPIKYVINTH
                     MHPDHVLGNAAFSQDEPEFVGHYKLARALGTRKESYLAANEQMLGPEAFEGSEIILPT
                     RLIKEPTTLDLGDRELLLEPQATAHTDNDLIVTDTKTDTLFLGDLLFSVHVPTIDGSI
                     KGWLALLDEIGKRNAARVVPGHGPKTMELPQALVPEQRYLKAIADDVRRQIAEGKTLS
                     EATKTAGFSEKDAWKLFGQYHVRNVTAAFAELEWD"
     gene            18584..19408
                     /locus_tag="AC16Y7_07175"
     CDS             18584..19408
                     /locus_tag="AC16Y7_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein dehydrogenase-associated SoxYZ-like
                     carrier"
                     /protein_id="HIR5709198.1"
                     /translation="MCNGLKGLTRSFAVVVLAAGVAIASTPPLQAEENMWPTLKAEAF
                     GDRPIQEEDGMVVLETPQKVEDAALVPLTVRVPPHVKGKLKSLSLFIDNNPDPKVATL
                     TYGPAAGTGGERSFSTRVRVDNFSHARAVLELEDGSLHMATKFLAAAGGCAAMQAKDP
                     EADVAGMGKTIVRTFTPALKSSPIWSGQVMVKHPNHNGMQLDINTAKFIPALYVKEMT
                     VTRDGELVFKLDGTFSISTNPNFRFTFGKGEKNDLDVVIVDTDGTEFTGQTQASGS"
     gene            19398..20015
                     /locus_tag="AC16Y7_07180"
     CDS             19398..20015
                     /locus_tag="AC16Y7_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5709199.1"
                     /translation="MDLKPATECSGAARPETPPVSTRGLLLKEAEVLLRTRGYAAFSY
                     ADLSARVGIRKASIHHHFRTKEELGVALIDAYLVEFEVALAEILKDEPQALPRLRRYA
                     AFFTGSLQDGMMPLCGALSAEAFALPASMQQRVRHFFELHLDWLEGVLRDGVTERALR
                     ADLDVRRAAAMVLSVLEGASLVAWALKDDAAMEPAFEQAVSSLKA"
     gene            20093..20407
                     /locus_tag="AC16Y7_07185"
     CDS             20093..20407
                     /locus_tag="AC16Y7_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709200.1"
                     /translation="MMVVLRFLPALTLIAAAQAVAYDGFEADFAACTQGSNKAEVVAA
                     CTRLIDNAKAENAVTGMFYGLRAANNGDPAQNCSDAQKSLSLADDDAIKGLSQQLIDA
                     NC"
     gene            20506..21456
                     /locus_tag="AC16Y7_07190"
     CDS             20506..21456
                     /locus_tag="AC16Y7_07190"
                     /inference="COORDINATES: protein motif:HMM:NF016228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HIR5709201.1"
                     /translation="MLGATGLIGAAVSARLQAEGHHVVRLARSVRGNGIVADLAMLTH
                     EEDWHPHLKGIDAVVNCAGVLQDGPRDQTAKVHAEAVGALVRACEALGVKRIVHISAI
                     GVDRTAPTEFSRKKLEGDKALMARGVDWVILRPSVVTGRGAFGGSALLRGLAALPVLP
                     APPDVQPFQVVQLDDLVETVLFFLRPDAPAKLMLEPTTPDRLSFVEVIAAYRRWLGWK
                     PATVVRMPAVLARLVYGVGDLLSWLGWRPPLRSTARLELERGVAGDPAAWIARASSRV
                     RFMMRSPQSLPPSKSGGLPGFTSSSPSFLGCWFCTGSLPA"
     gene            21423..21638
                     /locus_tag="AC16Y7_07195"
     CDS             21423..21638
                     /locus_tag="AC16Y7_07195"
                     /inference="COORDINATES: protein motif:HMM:NF025154.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX-like family protein"
                     /protein_id="HIR5709202.1"
                     /translation="MLVLYWIATGVIALGPGWEAGLDLMRRSGISEIGPRLIALGAAA
                     DILIGLGIAYRPTARLALLAALCLSIA"
BASE COUNT         3999 a         6772 c         6844 g         4061 t
ORIGIN      
        1 gccttggaag gtttgcggtt cgcgcacggc gatatcggcc agcactttgc gatcgagttc
       61 gatcccggcc ttcttcagac cgtcgatcag ctggccgtag gtggtgccgt tggcgcgcgc
      121 ggcggcattg atgcgctgga tccacaggct gcggaaggtg cgcttcttca ccttgcggtc
      181 gcggtaggcg tattgacccg ctttctccac ggcctgcttg gcgacgcgga tggtgttctt
      241 acggcggccg taatagccct tcgccttggt cagcaccttg cggtgacggg catgggcggt
      301 aacgccacgt tttacacggg acatagcagt gctccttaag ctcgtctcgc cttaagcctt
      361 aggcgtaagg gaggaaactc ttcttcacga tcttggcgtt ttcgtcggca aggaccatcg
      421 tcccccgggc gttccggatg aacttgttgg tccgcttgat catgccgtgc tgcttgccgg
      481 cagcggcgca tttcaccttg ccggtggccg tgaccttgaa tcgctttttg gcaccgctct
      541 tggtcttgag cttgggcatt tggcttcctt tttgggccgg cgctggcgac aggccgcccg
      601 gcgttcttga attcgatgat ggagagcaaa cgagccgcca cggcatgccc tgccgggcgg
      661 ctcctgaagg tgcgggttat agagggattt cagcctctat ggcaagcgcc caggtctttt
      721 gggcccaggc cgtcccctag gcccgaatcg gggccagcac catgaccatc tggcggcctt
      781 ccagccgggg ctccgactcc accttggcga tctccgcagt gtcgtctttc accttgttca
      841 gcagctggac gcccaggtcc tgatgggcca tttcgcggcc ccggaagcgc aacgtcacct
      901 tgaccttgtc gccttcctcg aagaaccggt tcatcgagcg catcttcacg tcgtagtcgt
      961 gcgtgtcgat attgggccgc atcttgatct ctttgatctc gaccgtgcgc tgcttcttgc
     1021 gcgcctcggc ggccttcttc tgcgcctggt atttgtattt gccgaagtcc aggatcttgc
     1081 agaccggcgg cttggaattg ggcgaaatct caacgagatc gagccccgca tccgccgcct
     1141 gggccaatgc gtcttcgata ccaacgacgc cgacattttc acctgtcgcg tcaatgagct
     1201 gaatttcagg agtgtcgatt ccctcgttta cacggggacc gcccctgttc ggggcaagct
     1261 tcatgggccg tcttacgatg tgtcatctcc tctagtgcag ttggcgccct cgccgcggcg
     1321 aggcacgggg ccgccggagc cagtgtcgat cgagagttct acaggaaggg aaatagacct
     1381 gacaagtcac gccggagcca tgcgacctgg caataaccat gaaaccggcc gaatccggca
     1441 cgtcgtggat tggtccgcgg tcggctaggc cggggtcgcc gggtccccgt gcatgacagg
     1501 gacgggcgca tcaaacgtgc cccagcgccg ctgcgcgcgg cgcatgatct ggctcacgag
     1561 gcgcacggcc tcgccgctca cgaagctcga tgtgctgttt tccagtgcga cgggacgcac
     1621 aaagcacgtt tcgtgtcgcg catcgtgcca tgtctcccac agcttctgat cgatcatgtt
     1681 ttccgacacg atgttggaga cggtgtcgcg aggcagcagc tcgagcgtga gaggcgtcca
     1741 gccggcgccg cgtgcgagct cggtcagcgt gttgtgcgga cgcgcattgg cggagatccc
     1801 gagttgctcg ccgagcacgc cgacaaactc gtcgcagagc tggtagtcgc cgacgaacca
     1861 gaaggtcgac aggaaggcgt tcgccaggtc gtacttctcg tcgtcggaga ggctcgcgat
     1921 ctgcgtccag gtcagctcga ggaagttctt caggatgtaa tgcgtaatga aattacgctg
     1981 catcgcactg tacgccacgt ccacgccgta aggctgcaga ccctgggaga tgtaacgggt
     2041 catccggtag ttgtagtagg agacgaagtg gctggcggga tcgcgcagca gtagactgcg
     2101 cttgatcaac cgccgctcga agagcggatc gaccgaaatt ccaagatagt ggccgctgag
     2161 cacgcgaatg tcgttcgggc tggtgatgtc cgcgccgtca tatctgctcg cgaagcgcct
     2221 gagcccccgc cgccgccgca gacggtgata ggccgattgg ggcagagact gcgccagatg
     2281 acggtcaatc gtccggccgg cacatttagg gacgtgatac acaaaatgca ccggcggggc
     2341 acttgccgcg cggtgcaaat gagccggtat ttctgacact acccccaaag acatagccgc
     2401 aagcttgaca tcgccatggt taacaaagcc tttcgagatt ggttccagcg ggggtgcgct
     2461 caaggtgtgt tcgccatcaa ggcagtgggt cggggcggga acggggcaga aactgggcag
     2521 tccgccgaaa tttcacgttt caacgaagat gtgacgcgag attggttccc agcagctcgt
     2581 cataaacagc gagcgtgtcc cgctgcatgg tcttaagggc aaagtgacag ccgacatggg
     2641 cgcgggccct ttgacagatt gtcgcacgtg cgtccggtgg gagcgccagc gcctcggcaa
     2701 gactctctgc taatgcggga gcgtcgctgg gaggtacaac ccaacccgtg aagtcgctgc
     2761 tgtccgaggc cgcgattacg ttctctggcg cagcgccgac atcggtgacg atgacggggc
     2821 atccggccgc ttgcgcctcg atactgggcc gcccgaatgt ctccgcgcgg gtcgatggaa
     2881 gaatgcccac cgtggcgagg ttgaacgcgg caggcatgtc gtcgcaatgg ccgaccaacc
     2941 gcacgcggcc ctcaagtccg ccgtccgcga tcttcttttc aagctcctcg cggtagccgt
     3001 cgcggccgtc agcgctgccg gcgaagatga ccacggcgtc tcccagctgg cccgcctcgc
     3061 gcaacagccg cgccgcctcg atcgtgtggg tatgaccctt cagccgggtc aggcgtgcgg
     3121 cttgcagaat gaccgggcat ccctcctcga tgccccaggc atcacggagg cgctggaacc
     3181 gcgcgggcgc gatggcgtcg cgggcgaact gggcgatgtc gagaccgcga tggatcacac
     3241 gcatgcgctc catcggcacg ccatggcgcg cggcaatgat ctccgccgtg tagcgcgagt
     3301 tggcaatgac gcgatccccc cgcgccataa cgccgttata ggcctgcttc agcggaaact
     3361 gtcttcgata ggcgccgtga taggtggtca cgaacggccg ccccgtcctt cgcgcggcga
     3421 ggaatgcgct ccaggcaggc gcgcggctcc gcgcatgcaa aagcgagacg tcccgcgcct
     3481 cgacaagctc tgcaaggcgc tgcgcgttag cgagaatggt cagcgggttc ttgcttgcaa
     3541 gcggcagacg aatgagttcg ccgccggcgt tttcgagacg ctctacgagt cttccgccct
     3601 cggaagccac caaggctttc gcgcccgcgg cggtcagcgc ttcggcgatc tcgatggtcg
     3661 attgctcgct gcccccggct tcgaggcgcg gcacgacctg caaaatggtc acctctgaca
     3721 tcgctttttc ttggcctttg ccttgcgcga agagccttgg atacgctagc gctcgtctcc
     3781 atgcagcatg aaccacagtt cgtcgaaatt ggccaagact cgaagcgcca agcatcgaag
     3841 cgccgccgca tcgcctatcg cttcgacgaa gggcaagacg cgtccagccc ggccgtgttc
     3901 tggctgtcgg gattcttgtc cgacatggcg agcaccaagg tgtcggcctt gagcgcatgg
     3961 gcgaagcacg aaggccacgc catgctctgt ttcgactatt cgggccatgg cgtttcgggc
     4021 ggggacatct tgaaggcctc cgtgggcgac tggctcgaag aagccgccac catgcttgag
     4081 ctgctcaaag gcaggcgggt gatcctcgtc ggatcgtccc tgggcggctg gattgggctc
     4141 ctgctggcgc gcgcgcttgc caaaagcgga gacgcgcgcc tcggcgggct cgtgctcatc
     4201 gcgcccgcct gggacatgac cgagaagctc atgtgggaga agatgtcgga gaagacgcgg
     4261 gtcatcgtcg agacggaggg cgtcttctac gcaccgtcgg actacgacga cgcctatccg
     4321 ctcaccaagg ttttgatcga agagggccgc aaccatctcc tcgcggcggg cggcatcgag
     4381 gtgggctgcc ccgttcgcat cctgcaaggc ctgcgcgacg aagacgtgcc ttggcagcat
     4441 gcgctggcgc ttgtcgatct catcgaaggc gacgacgtgg agatcacgct cgtcaaggac
     4501 ggcaaccatc gcctgtcgag cccggaagac ttgcggcggc tcacgcgcac ggtcggcgcc
     4561 ctcatcgaga cggcggcgtc tccttcggca taagcgatct cagtccttcg cggaacgtcg
     4621 gatagcggag ctcgacgccg agctccgatt tgatcagcgc gttgccaatc ttgcggctgc
     4681 cctggtagaa gctccgcgcc aacggcgtga gatccgcgtc ctcgaacgcg atctccggcg
     4741 gcggcgcaac gccgatcatc tgcgccacat gcgccaccac gtcgccaggc gcggcgggct
     4801 cgtcatcagc cacattgtag atcgcgccgg cacgaggttt cgcgatcgag gcttcgagca
     4861 cgcgcgcgat gtcgtccaca tggacgcggc tgaacacctg gccgtctttg ttgatgcgcc
     4921 gtgcggtgcc ggccttgagc ttgtcgaaca cgctgcgccc cggcccgtaa atgccggcaa
     4981 ggcggaacac ctgcaccggc acgccgatct tctcaccgaa gtcgagccag tcctgctcgg
     5041 ccacaacgcg cgcttcggtc cgcgcactgt tgggcttcgg gaccgtctcc tcgctgaccc
     5101 agttcccctg ctggtcgcca tagacgccca cggttgacag atagccgatc cactccaggg
     5161 tcttgagttt ggcgatctcg tctcggtagc tcgcgagcac cggatcgcct tcatcgcctg
     5221 gcggaaccga atgcagcaaa tgcgtcgtgc cttgcagcag cttcggcaat tcagaattgc
     5281 ttttgtcgcc ggcgaagcgt gtggcctcaa agccttctct gcgcaactgg ccgatcttcg
     5341 gttcttcgcg cgcggttccg gcaatctccc agccttggga ggcgagaagg ggcgcgagcg
     5401 cccgcgctga gaaaccgagg ccgaaagcga acaagcggct cacgcggcaa cctccagcca
     5461 ctcaccgcga acgtcactgt cactctcttg tccggcaaac gtagcgcgca agctgtgaaa
     5521 agtttcagga gctaatcgcg atagcgccca caccgccatg gcgcgcacca atggagactc
     5581 gtctgaaagt agcgcctcca cttttggtgc gagcgcggtg tcgcctgaat tgcctgcggc
     5641 gatcagcaca ttgcgcagga agcggttccg ccccgtacgc ttgatcggtg tgccgcgaaa
     5701 gcgtttgcga aagccagcat catccaacat gagcaacgca ttgagcggcg ggttgtctgc
     5761 gctcgcgcgc accgtgagct ttgcgtcatg cgagacttgc gcaaacttgt tccacgggca
     5821 cacggcaaga caatcgtcgc aaccatagat gcggttgccc atggccgcac gatattgcgg
     5881 cgcgatgtgt cctttgtgct cgatggtgag ataggagatg caagcccgcg catcgagctc
     5941 gtagggcgcg gtgaatgcat tcgtcggaca aacgtcgagg cagcgccggc attcgccgca
     6001 atgatcttct tccggctcat cgggcgcgat ttcggcggtg gtgaagatcg agccgagaaa
     6061 aagccaagag ccgaactccc gcgaaacaag attggtatgt ttgccttgcc agccgatacc
     6121 ggcccgcgct gcaagaggtt tctccatcac cggtgcggtg tcgacgaaca ctttcacgcc
     6181 cccttgcgtg agcgcttgta cgcgtccggc gagcactttc agattcttct tgatgacgtc
     6241 gtgatagtcg gcgcgtttgg cgtagatgga aaccgcgccg tgcgaacgct tccgaagagc
     6301 cgcgagcgga tcgccctcga cgccgtagtt caccgccaac acgatcacgc tttgtgcgtc
     6361 cggccacagc gcgcgcgggc tcttgcgtcg cgtggcggtc tcggcgagcc atgccatgtc
     6421 gccctggcgt ccttcggcga taaacctgtc gagcgcatcg ccgatgctcg ccggcacatt
     6481 ggcggacgta aagcgcacgg catcgaagcc aagagacttg gcttcctctg cgatcgttga
     6541 tcttagggta gctgtcatgc ccgcttgcca tacccgcatg atttggggcg cagcctcaga
     6601 agtcgagatt cgtgtatgtc tctgaaggcg tgaagccggc cacgcggtcc tgcaacagcg
     6661 gccggaaaga tggccgcgac ttgaccagcg cataccagtt cttcgcgggc tcgaactctt
     6721 cccaggggac ttctccgaga taatccaacg ccgacagatg cgccgcggcc gcgaggtcgg
     6781 caaagctcat gacatcgccc gcgagccagc tccgggtctc ggtcagatga ccgacatagg
     6841 tcaaatgata ccgcagattg tcgtgcccgg cccgcatggc ctccatatcg ggggaggcgc
     6901 tttgcggccc aaagcggcgg aagaccttct ccactactag atagtcggtc acttcgtcgt
     6961 ggaacttgtg gtggatccag tccaccaacc gccgcgcctc ggctcgctcg gcaaggccgc
     7021 ccggaaaggg ctgaaagcca cgggggtcgc gggcctccgc cgtgtcgccg agatactcgg
     7081 agatcgcata gctccctgcg atcggcgcgc cctcgtccgg aacgaagaca ggcagcgtcc
     7141 cggccgggtt tagggccaaa agctcctggc gccattccca gggcttttcc tcggtcaacg
     7201 cgacctccag cccgcattcg gccaaagcga ggcgaattga cctagagaag gcacatagag
     7261 gatggtgatg aagattcgcc attaatggct gtggcggccg ttcggtcgga aatgaatcgc
     7321 gcggggctag agcctcgtat agaacctgca cgcacgtcga acaagcggcg cgacgagggg
     7381 ctatgaccgt agagcagatc attgtgcttg ccgtggtgca ggggatcacc gagttcctcc
     7441 ccatctcgtc gtccggccat ctgattcttg ttccctatct gctccactgg gccgatcagg
     7501 ggcagttcgt cgacgtcatg gtgcatgtgg gcaccctgtt cgcgatcctc atctactttt
     7561 ggagagacgt ttggcagatc acgctgggcg ggctcgaact gttcaagggc aaggtcaccc
     7621 acgagggcaa gctcgccatc tacatcgtgc tcgcgaccat cccggcggtc gccttcgggc
     7681 tgttcctgaa gaagttcggc ttcggcgact tggagcgcag cgtcacggtc gttgcttgga
     7741 acacggtcct ctatggcttg ctgatgcttg tggccgacat ggtcggaccg caagagaaga
     7801 ccatcgagaa tatgaccttg cgcagcgcgc tgatcatcgg cgtggcgcag gcgctcgcgc
     7861 tgatccccgg cacgagccgg tcgggcgtca ccatgacggc ggcgcggttc cttggcttca
     7921 cgcggcccga cgcggcgcgc ttctcgttcc tgctcggcat tccagccatc gccggtgcgg
     7981 gcgtgctgac ggtcggcgag gcgttggccg cgggcgacac catcaccatg gatgcggtct
     8041 attgcgcgat cctgaccttc ttcgcagggc tcgcggccat cgctttcctg atggccttgg
     8101 tcaagcggat cagcttcctg cccttcgtac tttatcggat ggttttgggc ggcttcctgc
     8161 tggtgatgat ctacacctag gcgcgcgctc gtcctacgcc ttgtagatgg agaactgccc
     8221 gcgcgggcgg aactgctcca gatagtccgg cacgacattg gcgatctgca ccggctcgat
     8281 gccgaggcca tccagcgtgc gcttggcctt gatcgcagcc gcactcacca cgttgtcggt
     8341 ctcaagcaag cgcacctgat cgagggtaag cggcgggttc ggcagccatt gcagaaccgc
     8401 gccttgcagc ttggcgatcc agaacggcag cggcaccaaa ggacgcgagc gcatcgagta
     8461 agcgagcacg cgctccatga cctggcgcat ggtgaggatc tccgcaccgc caagctcgta
     8521 gggcgccttg gtactcgtgc ggccttccag cgccgccacg atgctctgcg caacgttgcc
     8581 gacgaagacc ggctggaact tggtgtgccc gccgccgatc agcggcagca ttggcgatag
     8641 gcgcgcaagc gccgcgaagc gattgaagaa ttcgtcctca ggtccgaaga cgacagaggg
     8701 acggaagatc gtggcgtcct tgtagacgtc cttgatggcc gcttcgcccg cggctttgct
     8761 gcgggcatag atcgaggctg agctcgcgtc ggccccgatg gccgaaacgt ggaccagcgt
     8821 tccgacgccg gccgccttcg cggcctccgc gacattgcgc gcgccctcgt cctgcacgga
     8881 cttgaaggtc tgtttgccgg tgggtaacag gatgccgacg aggttgacga cggcgtctgc
     8941 gtcctgtgcg gcggccagca gcgagtcggg aaagcggacc ttggcctgga ccggcacgat
     9001 ctggccgacc ccgcctaagg gctggagatg gccggcaagc tccggccgcc ggacggcgac
     9061 ccggatgcgt gcgcccgtct gcgcgatggc ccggaccgtg tgccggccca agaagcccga
     9121 gcccccgtag accgttacca acgcccgatt attgagtgcg atgcccactt agatgcctct
     9181 taaccccgac gcgctatggt gccgctcagc attacacgag ggatgctgtg ccttgaaggg
     9241 aactcttcag gcaattgaca atgggatgca ccgatcgtac ctgaacgcct tcaagcccag
     9301 gtggcggaat tggtagacgc gctagcttca ggtgctagtg cccgcaaggg cgtggaagtt
     9361 cgagtcttct cctgggcacc aaattcgccg cgcccccttt tgctgagggg gtgccggccg
     9421 ctaacgtcga gaagcccttg agccagctaa agaatgtcga gctttatcag ggcgatctgc
     9481 cggaaggccg cttcacggcc aagacgatcg ccatcgacac cgagacgctg gggctgaacc
     9541 cgcatcgcga ccggctctgc cttgtccagc tctccgacgg ggacggcacg gccgccctgg
     9601 tccaggttaa ggccggccag gatgcccccg agctcaagcg cctcctggcc gatgagagcg
     9661 tcctcaagct gtttcatttc gcccggttcg acttggcggc gctgaacaat ttcttcggcg
     9721 tcatggccgg gcccgtctac tgcaccaaaa tcgcctccaa gctcgccagg acctacacgg
     9781 accgccacgg cctaaaggac ctggtccgtg agcttcttgg cgtggacctg tccaagcagc
     9841 agcaatcctc cgattggggc gcggcggagc tgagccccga gcagctcaat tatgcggcga
     9901 gtgatgtgct atatctgcac gcgctgcgag atcgtctcga cctcatgctg aagcgggagg
     9961 gccgaacgca tttggcgaag gcctgcttcg aatttctccc cacccgggcc gagctcgatc
    10021 tcgaaggctg ggccgaaatg gacatcttcg cccactagaa ggctgctagg gccccagcaa
    10081 tccaaggcct tcggtcgcaa tctgatcaaa gtcctgcggc tagtgtggcg gggtcagcga
    10141 gacactggcg ggtaccagta ccatggcata tgaggcgaat ctcggcacgc gaggccggca
    10201 ctacgttgtg cgcacgcccg aggagcgtca gcgtgccttc cgtagcgcca ggcgccacag
    10261 cttctttgtg tcctttcttc gccgggttct gcccgtcctc gccctgctcg tacttgcggc
    10321 ctatttcatc tccacgcgca tgaacatcac ggtcggcgat ttcaccgcca gcattgacgg
    10381 gatggaggtc gtcgacggga acttgcgtat gctcaacccg acgctggagg gggccgacaa
    10441 gaagaacggc aagtacgtgg tgagggccga atatgcggac caggacatca ccagccctga
    10501 cattatcaag ctccacgcca tcagggccga tatcgacgac ccgaccggcg gctggtccaa
    10561 gatgcaggcg gttcgcggcg tcttcaacaa cacggccgag cgcctcatca tgaaggatcg
    10621 catcaccgtc gccaccagtt ctggcgtcac cggcgagctg aaatacgcca cgatggacat
    10681 ggcaacgcag acgctccgtt cccacagtcc cgtcttcttc gagctgaaca atggcaacgc
    10741 caccgtccgt gcgaacgcga tgacgctgcg ctccaaggag aaggtcatta cgttccgggg
    10801 caaggtcgcc gttcacctcg agccgaaaaa ggttgctgcc gcgaagaagc ccgcgcaaca
    10861 gccggtgccg gcggcgaacg gcgcagctca gagcggcgca gcaccaaccg gcgcggcgca
    10921 ggtgccgccg caatgagcgg gtgtggtgca attcggcagg ctcttcacgt gctcggcctg
    10981 ggcgtcgttc tcttgttgcc agtaagcccg cttaccgcgc agaccatgac caactcgttt
    11041 ggcgggatgt ccaaaaattc ggacgccccc atcgatatcc aatcggactt gctcaccgtc
    11101 tacgacacaa agaagatcgc gatcttcaag ggcaacgtga aggctgtgca ggggacgacg
    11161 acgctccgcg ccacgcagct cgacgtgcac tatatcggcg gcgcggaaga gctgacgggt
    11221 aaaggcgcgg accaagacaa cgcgaccaca ccggtcgctc agcagggcgc gacccagaag
    11281 gatgcggatc cgcaatcgag catcaacaag cttgaagcgc gcggagacgt ctacatcacc
    11341 agcgaggacg accaaaccac gtcgagcgac tgggcggtct atgatgtgcc ggcgcagctc
    11401 gtaaccgtag gtggtaatgt ggtgttatcc caaggacaga atgtgctaaa ggggaaccgg
    11461 ctcataatcg acctaaagac aggtgagagt cggttcgaca acacgggaaa cgctgccgcc
    11521 ggaggcggtc ggattcgtgc actgttcatg cccaagggaa gcgacgttaa ggacagtgcc
    11581 gcggcgatgg agccggagcc cggccaggcc cagcaagtgc caggggcaca agtgccgggg
    11641 gcagcagtcg agactggcga gccgttgccg attgcgccgc cgcgccggta aacccgttca
    11701 agcgcttcaa ccgatattaa ccggccagcg cttcaactga ggagcgaaag gggtcagtgg
    11761 gcaagttgtt gtcgtttcca ggtcatcagg gcaccgcggc ccaaggagcg cccgcgcaag
    11821 gggcgccgct tgcgcatggc caggccccgc tacgagagcc cggcaacggc tggctgacca
    11881 tccgctcggt cgtcaaaagc ttcaagaaac gcatggtcgt gcgcggcgtt agcctcgacc
    11941 tggcgcgcgg cgaggctgtc ggcctcttgg gtcccaacgg cgccggaaaa accacggtgt
    12001 tctacatgat taccgggctg attccggccg atgaagggtc catcaagatc gacggacgcg
    12061 acgtgacgag actgccgatg taccggcgcg cgcggttggg tatcggctat ctgccgcaag
    12121 aagcctcgat cttccgtggc ctcaccgtgg aggagaacat tctcgccgtg ctcgagatgg
    12181 tcgagcccaa gaggaaggaa cgcaagaagc agctcgatgc gttgcttgag gagttccgta
    12241 ttacgcgtct aagaaagtcg ccgtccatgg cgctgtcggg cggtgagcgc cgccgcgtgg
    12301 agattgcccg cgcgctcgcc tcacgcccgt cattcatgct tctcgacgaa cccttcgccg
    12361 gcatcgatcc catagccgtc ggcgacattc gcgcgctcgt gcgccacctc accgagcgcg
    12421 gcatcggcgt tcttatcacc gaccacaacg tgcgtgagac gctcgacctt gtgagccggg
    12481 cctacattat tcacgacggc atggtgctga cccacggcac caccgacgac atcatcaaca
    12541 acgaggatgt gcggcgcgtc tatcttggtg accagttcaa gatctaagtg ctcgatcgcg
    12601 agtgagtccg taaatggcga tcaatacaaa gctggagctc cgccaggcac acggtctggt
    12661 catgacgccg caactgcagc aggcgatcaa actgctgcag ctctccaaca tggaacttgc
    12721 gacgtttgtc gagggtgagc ttgagcgcaa tccgctgctg gagcaggccg acgcggacga
    12781 accgagcaac acggccgatc aaccctcacc tgcagccaag agcgaggaag gaagttcgga
    12841 agccacgggc gcagagtcag gcaccagcga cgaaggagac tgggtcaatc tcgaaaccgg
    12901 aaacgattcc ggccgcaccg acgatctcga tgccgaaccg caggacgtgt ttcccgatgc
    12961 ggagaacttc acccccggcg cggtaagcga ttccggttgg agtggactga cgcaagggcc
    13021 gcgccccgac atcgaccaag gcaataatct tgaagcctac gtggctgagg agaagacgct
    13081 tcgcgatcat ctcgccgagc aattgtcgtt ggcgttctcc gatcctgcgc ggcggctcat
    13141 tggccagcac atcatcgata tgaccgacga ggcgggttac ctgcgcggcg agctcgatgc
    13201 gctcgcagag ctgctgggtg ctccgcccga gcttattgaa gagacgttga aggtgatgca
    13261 gagctttgag ccatgcggcg tattcgcacg cgatcttcgc gaatgtctca cgcttcagct
    13321 gaaggaacag gaccgctgcg atccggcgat ggcgggactg atcgacaatc tccacctcct
    13381 tgccacgcac gacctcgttg cgttgaagcg cgcggcccat gtgtcggatg aggatctctg
    13441 cgacatgatc gccgaggtca ggcgtctaaa cccgaagccg ggcctgaagt atggctccag
    13501 ccccacccag cccatcgtgc ccgacgttct cgtgcgctcg cttcccgatg ggagttatta
    13561 cgtggagctg aacagtgaca cgcttccccg cgttctcgtc aaccagtcct actacgcgac
    13621 ggtctcgaag tcggccctgc gcaaggagga caagagttac ctggtggatt gcctccagac
    13681 cgcgaactgg ctggtgaaga gtttggacca gcgggcgcgc accattctca aggtttcgca
    13741 ggagatcgtg cgtcagcagg atgcgttctt cacctatggc gtccggcatt tgcggccgct
    13801 caatctaaag accgtcgccg atgccatctc catgcatgag tcgacggtga gtcgagtgac
    13861 cgccaacaaa tacatggcga ccaatcgcgg cctcttcgag ctcaaatact tcttcacgtc
    13921 ggcgatcccc gccacgtcgg aagggggcac gcattcttcg gaggccgtgc gccaccgcat
    13981 tcgcgagatg gtcgacgcgg aaccggcggc ggaggtgttg tccgacgaca aaatcgtcga
    14041 acggctcaag ggcgacggca tcgatattgc acgccgcacg gtggcgaagt atcgcgaagc
    14101 cttggggatt ccgtcttcgg tgcagcgccg gcggctcaag cggatcgcca tgggcgcagg
    14161 cgcctgatgg ggacgtctaa cacactgttc gtcaatgcga atcgtgtaaa gagggtggat
    14221 cgaattcatt gcgtttgaca cccttaagac aggggtctag cttccgccac cgcgatcgag
    14281 gctggttgct gggccggggc gtttagaaag cgcgctgacg aatcgagcct cggaaatttg
    14341 gatgcgcgcc gggaagcgct ggtcagtcag gatttgacag tcttccacgg gcatgacgac
    14401 accgattacc ggaaagatac tggaacgttg gtaggcgttg cgcggttgcg ttcagggacg
    14461 cgttgtacgg atcatcgagc attgtgtggg gcacgcgccg cggggtacgt gcttttcgaa
    14521 caagggtaac gggagttccg cagctactct tcctgcggct attgaaagcg cgggcggatg
    14581 gggccagctg tcaacccatc ggctcctttg cggtcacacg ggtatcagaa tgcatctagg
    14641 tgactttatc tcacccgaag cagtgatggc ttcgctcaag gtcaagaaca agaagcagct
    14701 tctgacggag ctggccgcgc gcgtcgcgcc gatggccggc gttccggagc gcgagatctt
    14761 cgacgtcctg cttcaacgcg agcggctcgg ctccaccggg ctcggcaatg gcgttgcgat
    14821 ccctcatggc aagatggccg gactgcctca gatcgtcggc ttgttcgcgc acttggccga
    14881 accgatcgac ttcgagtccg tcgacggtga gccggtcgac atcgtgtttc tgctgcttgc
    14941 gcccgaaggc gccggtgcgg atcacttgaa ggccttggcg cggatttcgc ggctgctgcg
    15001 cgacgaggcc gctgtgaaga agctccgcgc gtcagaagac gccgccgcgc tctacgcgct
    15061 cctcaccgaa ggcgaggctg cctcgcacgc ggcctagcgc ggcgccagcg tcgcaatcat
    15121 ctcgttgacg gcctcggcca tgcccatggc ggcggcgaag tcgaacttgt tgatggccac
    15181 gaacgccgcc accccgcgtg ttggtgcgaa ggcgatgtag gtgaacgtcc cttggagtcc
    15241 tcctgccttc tgcaggatca gcggccggtt cccctcgggc atcatcacca cccacccaag
    15301 gctcatcgcg tccatgtgcc cggactcatc catccctgaa acgggatcga ggccgtcacg
    15361 gacgagatag gccgcgtggt ccagcatccg cacctcggca tcctcgggcg acaggcggtc
    15421 cagatgccac tgcatccagc gcagcaaatc cttcggcgtg gagtaaaggc cgccagagcc
    15481 caagatgacg tctccgctcg gcacgttcgg caacgccttt ccgtcgaagc cgtgcccttg
    15541 catgaacctt ttcctctgcg cgtcggtcag cacgaaactc gtatcgtcca tgccgagcgg
    15601 gctggtgacg tgtttcttca ggaggtcggg atagggctcc ttcgctgctt cggacaaggc
    15661 gatggacagc aaatcgaagg caaagttcga gtagagcacg cccgtgcctg gcgggaagag
    15721 cagcgtctcg gtcttcagcc attcggcgaa ggcatcgcgc gtgatcggcg cgaatggatc
    15781 gtcgggcggg ccgtgctcgt gcgggacttc gcgcggcagc ccggacgatt gcgtggcgat
    15841 gtcgatcaac cggagcttgg cgacgttcgg gtccgcgccg cgcccaagat ctggcgccgt
    15901 cttgcccacg gtctgggtca acgacacggt gccgtcggcc gcaagcgccg cgagcacttg
    15961 gcccgtgaaa gccttggtga tcgagccgat gcgtaagacc gtatcgccgt caggcgcatc
    16021 gccgccatcg ctccgttcgc ccacgccgtg gatggcgacg tcgccgcccc gcaccgcgcc
    16081 gataacgagc cccgggactt tcgtctgcag atagagaaca tcgccggtga aggtgacgac
    16141 ctcgtcgagc agggcgtccg catgggcctg cgtcgatacg ccaaggaaac tgcttgcggt
    16201 caccagcagc gcgaagatcg cgcaccgcat aggctgaatt tgggaaacat gagacatcgt
    16261 gtgcaccatc cagaagaatc tgatggcgac tatgcgcaag tccgtggggg ttcgctaggg
    16321 cgcgcgccga tcttggataa agctcagtgc acgctcaccg ggacgagatc gttctcgatg
    16381 gcgttggcga tggccgcagt acggctgtcg gccagcgcga tcggcgtgcc gtctgcactg
    16441 agcacggcaa acagatcgat gccctctagg gcctcgtcga gcgtggggaa cagctgctcc
    16501 gcagcgtccg tgtcgacctg cttgatataa gcaacctcgc cgtcacccag gagcgcaagc
    16561 tccttcaggc tcatcgggcg ggtctcgttg ttttccgtct tgtccataaa agtcttactt
    16621 cctcatggaa gagacgcggc tgctgtcctt ccgcaaaacg cacggctcaa cctgctggcg
    16681 tgatccaaac cttgaggaca cgcttctccc tatggggccg ttccagctcg atggcaagca
    16741 ggccatcgtg caactcggcc ttgcggactt ccacgccctt ggccagggcg aaggtccgtt
    16801 cgaactttcg cgcggcgatg ccgcggtgaa gatagtcctt cggaccttcc tcgcgcttgg
    16861 cgccgcgaat tgcaagctct ccgttctcga tgctgacttc caattcgtcg cggccgaacc
    16921 ctgcgaccgc cagcgtgatg cgcaaggcct cctggccaca atcgctatcg tggagaagct
    16981 ccacgttgta gggcggatag ccgtcttccg aagttttcgc ccgcgcgtcc aaccggcctg
    17041 tcgataggcc taaccaaaga ttgccgtcga gaagtgccat gcgcagggtg tccgggcgga
    17101 aagccccaaa attcggtcag cgccgcgcat ggacggcaaa atgcgctgga ggaggccccg
    17161 gtcggcgcct cgtacgatct tttgccctgc gttggccctg agccttaatt aacctgctgg
    17221 catatgggaa attccactcc atattcaagc tggacgcatg atgccccaac agccgaattg
    17281 gatgctttcc gccatgacat tcttcgtgaa ccccaacact ggcaaccaca gcaatgatgg
    17341 ccgtagcgag aagacggctt tcaggaacct gtcccacgct ctcgatgccg ccgccgcagg
    17401 tgacaagatc ctggtgacgc ctgcgaccta tgacgaggat ctgcccaagc tcattgcgcg
    17461 tgcaaaggca gctggcatca cggtcgcagt gctgggcggg cactagctcc ggcgccttga
    17521 caggcaaacc tactagtagg tagggtcaac gtcataatgc caaagactga agaaacaaag
    17581 caggccgctt gcggatgcgg ggcgtatccc ttgtcccgcc gcaacctctt tggcgcggcg
    17641 ttggcgctcg cggccgcccc gcttctgcgc tacgaccctg cgcaggctgc ctctgtcgat
    17701 ccaagcgaag tggcgccggg catcttcgtc caccagggcc gctacgaggt gcagtcgccg
    17761 gacaatcggg gcgacattgc caatgccagt tttgttgtcg gcagcgacgg cgtcgccgtg
    17821 atcgatacgt ccggcagcgc caagatgggc gcggagctgc gcgaggccat ccgcgccgtc
    17881 accgacaagc cgatcaaata cgtgatcaac acgcatatgc atcccgacca tgtgttgggc
    17941 aacgccgcct tcagccaaga cgagcctgag ttcgtcggtc actacaagct cgcgcgtgcg
    18001 ctcggcaccc gcaaagagag ctacctggca gcgaacgaac agatgcttgg gccggaggcc
    18061 ttcgaaggca gcgagatcat cctgccgaca cgtctcatca aggagccgac cacgctcgat
    18121 ctcggcgacc gggaactgct gctcgagccg caagccacgg cgcacaccga caacgacttg
    18181 attgtcaccg acaccaagac cgatacgctg tttctcggcg atctgctgtt ctccgtgcac
    18241 gtgccgacga ttgacggctc catcaagggc tggctggcct tgctcgacga gatcggcaag
    18301 cgcaacgccg cacgcgtcgt gccgggccac ggccccaaga ccatggagct gccgcaagcg
    18361 ctcgtacccg agcagcgcta cctcaaggcg atcgcagacg acgtgcgccg tcagattgcg
    18421 gaagggaaga ccctatcgga ggccaccaag accgccgggt tctccgagaa ggacgcctgg
    18481 aagctgttcg ggcaatacca tgtgcgcaac gtcactgcgg cgttcgcaga acttgaatgg
    18541 gactagcgac aacgtcccgc cccatcgaag gagcacgaga ctcatgtgca atggactgaa
    18601 aggtctgacc cgaagtttcg ccgtcgtggt gctggctgcc ggcgtggcaa tcgcgtcgac
    18661 gccgccgctg caggccgaag agaacatgtg gccgacgctc aaggccgaag ccttcggcga
    18721 ccgtcccatc caagaagagg acggcatggt cgttctcgaa acgccgcaga aggtcgagga
    18781 cgccgccctc gtgccgttga cggttcgcgt gccgccacac gtgaagggca agcttaaatc
    18841 gctgtcgctg ttcatcgaca acaacccgga cccgaaagtg gcgacgctca cctacggccc
    18901 tgcagccggc acgggcggcg agcgcagctt ctccacgcgc gtgcgggtcg acaatttctc
    18961 acacgcccgg gctgtcctgg agctcgagga cggcagtctg cacatggcga cgaaattcct
    19021 ggctgccgcc ggcggttgcg ccgccatgca ggcgaaagac cccgaagcgg acgtagcggg
    19081 catgggcaag accatcgtgc gcaccttcac gcccgcgctg aagtcgagcc cgatctggtc
    19141 cggccaggtg atggtcaagc atcccaacca caacggcatg cagctcgaca tcaacaccgc
    19201 caagttcatc cccgcgcttt acgtgaagga gatgacggtg acgcgcgacg gcgagctcgt
    19261 gttcaagctg gacggcacgt tctcaatctc gacgaacccg aacttccgct tcaccttcgg
    19321 caagggtgag aagaacgatc tcgacgtcgt catcgtcgac acggacggaa ctgaattcac
    19381 cgggcagacg caggcaagtg gatcttaaac ccgccaccga gtgcagcggc gcggcaagac
    19441 cggagacgcc gccggtcagc acgcggggcc tgctgctgaa ggaagccgaa gtcttgttga
    19501 ggacgcgagg ctacgccgca ttcagctatg cggacctttc ggcgcgtgtc ggcattcgca
    19561 aggcaagcat ccaccaccac tttcgcacga aggaagagtt gggcgttgcc ctgatcgacg
    19621 cctacctggt cgagttcgag gtcgcgctcg ccgagatcct caaggacgag ccgcaagcgc
    19681 ttccgcgtct tcgccgttac gccgccttct tcaccggtag cctgcaggac ggcatgatgc
    19741 cgctttgcgg ggcgttgtcg gcggaggcct tcgcgcttcc ggcctccatg cagcagcgtg
    19801 tccggcactt cttcgagctg catctcgatt ggcttgaggg cgtgttgcgc gatggtgtta
    19861 ccgaacgcgc tcttcgtgcc gacctcgatg tcaggcgcgc cgctgccatg gtgctcagcg
    19921 tgctggaagg ggcgagcctt gtcgcctggg ctctaaagga cgatgcggcc atggagccgg
    19981 cattcgagca ggcggtctca agcctcaagg cgtgagtgac catttggggt cgattgcgta
    20041 ggcgcggacg cctacactcc tccgtattgg tcctttctcg gaatcggaga caatgatggt
    20101 cgtcctcaga tttttgccgg ctctgacttt gatcgctgcc gcgcaggcgg ttgcgtatga
    20161 cgggttcgaa gcggacttcg cggcgtgtac ccaagggagc aacaaagccg aagtcgtggc
    20221 ggcctgcaca cggctgatcg acaatgcgaa ggccgagaac gcggtcaccg ggatgttcta
    20281 cggcttaaga gcggcaaaca acggcgaccc cgcgcagaac tgttccgatg cccaaaagtc
    20341 cctgtcgctc gccgacgatg atgcgatcaa ggggctgtct cagcagctca tcgatgcaaa
    20401 ttgttgacgc cgatcttcgg acaagtcgtt ggtagcggat gatcctgatc ggggtcaggt
    20461 ccacagacct ctcggcggga cacacgagcc cctctcaacg tcctcgtgct tggcgccact
    20521 ggtctcattg gcgccgccgt ctcggcgcgc ctgcaggccg agggtcacca cgtcgtaaga
    20581 cttgcgcgct ccgtgcgtgg caatggcatc gtcgccgatc tcgccatgct gacacacgaa
    20641 gaagattggc atccgcacct gaagggaatc gatgcggtcg tgaactgtgc gggcgtccta
    20701 caggacggac cacgggacca gacggccaag gttcacgcgg aggctgtcgg cgctctcgtc
    20761 agggcctgcg aagcgctggg ggtgaagagg atcgttcaca tctcggcaat tggcgtcgac
    20821 cgaacggcgc ccacggaatt ctccaggaaa aagcttgaag gcgacaaggc cttgatggcg
    20881 cgcggcgtcg attgggtgat cttgcggccc tcggtcgtta cgggtcgcgg ggctttcggc
    20941 gggagcgcgc ttctgcgcgg acttgcggct cttcccgttc tgccggctcc acctgatgtg
    21001 cagccctttc aggtcgtgca gctggacgat cttgtcgaga ccgtgttgtt ctttctgagg
    21061 ccggacgcac ccgcaaagtt gatgttggaa ccaacgacgc ctgaccggct cagtttcgtc
    21121 gaagtgattg cggcctatcg tcgatggctc ggctggaagc cggcgactgt cgtacggatg
    21181 ccagcagtgt tggcccgcct cgtctacggg gtgggcgatt tgcttagctg gctcggttgg
    21241 cgtccgccgc tgcggagtac cgccaggctt gagcttgagc gcggtgtcgc cggcgatccc
    21301 gccgcgtgga tcgcgcgggc atccagccgc gttcgtttca tgatgcgctc gccgcagagc
    21361 ctgcctccgt ccaagagcgg tggtttgccc ggctttactt cctcaagccc gtcgtttttg
    21421 gggtgctggt tctgtactgg atcgctaccg gcgtgatcgc gctcggacca gggtgggagg
    21481 ccggcctgga tctgatgcgg cgaagcggca tctccgagat cggtccgcgt ctcattgcgt
    21541 tgggcgcggc tgccgacatt ctcataggac tgggtatcgc ctatcgtcct acggcgcgcc
    21601 tcgcgctgct tgcggctctc tgcctctcaa ttgcctaggt cgttgcgggg acggccttgc
    21661 tgccgcagct ctgggc
//
LOCUS       DBNHVU010000175        34710 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_656561, whole genome shotgun sequence.
ACCESSION   DBNHVU010000175 DBNHVU010000000
VERSION     DBNHVU010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 34710)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 34710)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34710
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_656561"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..847
                     /gene="rplC"
                     /locus_tag="AC16Y7_07200"
     CDS             62..847
                     /gene="rplC"
                     /locus_tag="AC16Y7_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIR5709203.1"
                     /translation="MRSGVIAQKVGMTRIFTDAGEHIPVTVLRLENCQVVAQRTAEKN
                     GYTAVQLGAGRPKVKRLTRAERGGFAVANVEPKRKVAEFRVSPENMIDVGAEITADHF
                     VEGQFVDASGTSIGKGFAGGMKRHGFGGLRASHGVSINHRSLGSTGQCQDPGKVFKGK
                     KMAGQMGSVAVTTQNLRVVRTDVDRGLIMIRGAVPGAKGGWVLLRDAVKRALPDAAPK
                     PGAFRKGADDQKPAKEAKLETKPEAAPEAPVPEAKPEEGGDKA"
     gene            847..1467
                     /gene="rplD"
                     /locus_tag="AC16Y7_07205"
     CDS             847..1467
                     /gene="rplD"
                     /locus_tag="AC16Y7_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR5709204.1"
                     /translation="MKADVTTLDAKKAGTIELSDDVFGLEPRADLLHRMVRYQLAARR
                     RGTVAVKDRSEIVGTTAKMFRQKGTGRARHGNAKPTIFRGGGKAFGPKPRSHDIELPK
                     KVRALALRHALSSKAKTDALVVIDSCEMKDPKTKALKARFDKLGVSSALVVDGAEVQD
                     GFARAARNIPHIDVLPVQGINVYDILRHDTLVLTKAALEALEARFK"
     gene            1464..1757
                     /locus_tag="AC16Y7_07210"
     CDS             1464..1757
                     /locus_tag="AC16Y7_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR5709205.1"
                     /translation="MSALQAYDVILSPVISEKSSEASESNHVVFRVRLDATKPQIKKA
                     VEKLFGVKVVAVNTLTRKGKTKRFRGIEGRQKDVKKAVVKLAEGESIDVTTGI"
     gene            1762..2604
                     /gene="rplB"
                     /locus_tag="AC16Y7_07215"
     CDS             1762..2604
                     /gene="rplB"
                     /locus_tag="AC16Y7_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR5709206.1"
                     /translation="MALKNFKPTSPSQRQLVLVDRSHLWKGKPVKSLTEGLNKSGGRN
                     NTGRVSIWHRGGGHKRSYRLVDFKRTKRGMPATVERIEYDPNRTAFIALIKYEDGDLA
                     YILAPQRLAPGDTVIADDKADVKPGNAMPLASMPIGTIVHNVEMKVGKGGQIARAAGS
                     YVQLVGRDAGYAILRLNSGETRMVPAGCMATVGAVSNPDNSNTSFAKAGRMRWKGRKP
                     VVRGVAMNPIDHPHGGGEGRTSGGRHPVTPWGKSTKGKRTRSQKGSDKFILRSRHQKK
                     KKKG"
     gene            2608..2886
                     /gene="rpsS"
                     /locus_tag="AC16Y7_07220"
     CDS             2608..2886
                     /gene="rpsS"
                     /locus_tag="AC16Y7_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003547552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIR5709207.1"
                     /translation="MARSIWKGPFVDGYLLKKAEKARQSGSNAVIKMWSRRSTILPQF
                     VGLTFGVHNGHKHVPVLVTEDMVGHKFGEFAPTRTYHGHTADRKAKRA"
     gene            2889..3269
                     /gene="rplV"
                     /locus_tag="AC16Y7_07225"
     CDS             2889..3269
                     /gene="rplV"
                     /locus_tag="AC16Y7_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HIR5709208.1"
                     /translation="MGKPQRERTLKDNEAKAVTRLIRVSPQKLNLVAQLIRGKKVDRA
                     LADLTFSRKRIAKDVKKTLESAIANAENNHDLDVDALIVSEAYVGKNIVMKRLRARAR
                     GRSARILKPFSQMTVVVRQVEESA"
     gene            3269..3988
                     /gene="rpsC"
                     /locus_tag="AC16Y7_07230"
     CDS             3269..3988
                     /gene="rpsC"
                     /locus_tag="AC16Y7_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007770147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR5709209.1"
                     /translation="MGQKVNPIGLRLGVNRTWDSRWFASGGEYAQLLHEDLKMREHIL
                     NSRKQAGISKVVIERPHKKCRVTVYTARPGILIGKKGADIEKLRRELATMTDSDVHLN
                     IVEVRKPEVDATLVAEGIAQQLERRVAFRRAMKRAVQSAIRMGALGIRINCGGRLGGA
                     EIARTEWYREGRVPLHTLRANIDYGTALGRTAYGIIGIKVWIFKGEIMEHDPMAQENR
                     AIEAQEGGRSTGRREPASARG"
     gene            4006..4419
                     /gene="rplP"
                     /locus_tag="AC16Y7_07235"
     CDS             4006..4419
                     /gene="rplP"
                     /locus_tag="AC16Y7_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR5709210.1"
                     /translation="MLQPKRTKYRKMQKGRIKGAAKGATALNFGTFGLKAQEPARVTA
                     RQIEAARRAMTRQMKRAGRVWIRVFPDVPVSKKPTEVRMGKGKGTPEFWAAKVKPGRI
                     MFEIDGVGESVAREALRLAAAKLPIKTRVVARTEG"
     gene            4423..4629
                     /gene="rpmC"
                     /locus_tag="AC16Y7_07240"
     CDS             4423..4629
                     /gene="rpmC"
                     /locus_tag="AC16Y7_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR5709211.1"
                     /translation="MKASQVHDMTIDQLNDELVKLKKEQFNLRFQAASGQLENTARVR
                     QVRRDIARVQTVARERDAAAKVKG"
     gene            4635..4880
                     /gene="rpsQ"
                     /locus_tag="AC16Y7_07245"
     CDS             4635..4880
                     /gene="rpsQ"
                     /locus_tag="AC16Y7_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007066372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR5709212.1"
                     /translation="MPKRVLTGVVVSDKNDKTVTVKIERRFTHPLFKKVVRRTKNYHA
                     HDEKNEFKVGEKVSIEECAPISRHKRWVVLPREQATA"
     gene            4928..5296
                     /gene="rplN"
                     /locus_tag="AC16Y7_07250"
     CDS             4928..5296
                     /gene="rplN"
                     /locus_tag="AC16Y7_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011567679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR5709213.1"
                     /translation="MIQMQTNLDVADNSGAKRVQCIKVLGGSKRKYASIGDTIVVSVK
                     EAIPRGRVKKGQVMKAVIVRTASGVRRPDGSLIRFDRNAAVLVTPQGEPVGTRIFGPV
                     TRELRAKKHMKIVSLAPEVL"
     gene            5299..5619
                     /gene="rplX"
                     /locus_tag="AC16Y7_07255"
     CDS             5299..5619
                     /gene="rplX"
                     /locus_tag="AC16Y7_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012171069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR5709214.1"
                     /translation="MRNLKIKKGDQVVVITGKDKGKHGEVLKVMPSENRAIVKGVSMV
                     RRHQRQTANQEGGIITKEAPIEISNIALEDPKDGKPTRAGYKFLKDGRKVRFARRSGE
                     VIDG"
     gene            5612..6169
                     /gene="rplE"
                     /locus_tag="AC16Y7_07260"
     CDS             5612..6169
                     /gene="rplE"
                     /locus_tag="AC16Y7_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR5709215.1"
                     /translation="MAEQVYVPRLKLQYQDVIKGDLSKEFSYPNVMQVPRLEKVVLNM
                     GVGDAVSDSKKVKAAAADLGLIAGQRPVITKARKSIAGFKLREGMPVGAKVTLRKDRM
                     YEFLDRLVNIALPRVRDFRGLSPKSFDGRGNYSMGLKEHIVFPEIDYDKVEDVLGMDI
                     IVCTTAQTDDEARALLKGLNFPFRS"
     gene            6198..6503
                     /gene="rpsN"
                     /locus_tag="AC16Y7_07265"
     CDS             6198..6503
                     /gene="rpsN"
                     /locus_tag="AC16Y7_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HIR5709216.1"
                     /translation="MAKTSMIERDKKRRRLAKKFAYRRARLKEIAADRSFPAEERFAA
                     RLKLAELPRNSSPTRIRNRCDLSGRPRGYYRKLRMSRIALRDLGSTGMIPGMVKSSW"
     gene            6513..6911
                     /gene="rpsH"
                     /locus_tag="AC16Y7_07270"
     CDS             6513..6911
                     /gene="rpsH"
                     /locus_tag="AC16Y7_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR5709217.1"
                     /translation="MNVNDPLGDMLTRIRNAQMRHKTKVTTPASKLRERVLDVLTEEG
                     YIRGYARVDYDGGKSEFEIELKYFDGEPVIKDIKRVSTPGRRVYSSVKDLPTVANGLG
                     VAILSTPKGVMSDSRARNENVGGEILCNVF"
     gene            6941..7474
                     /gene="rplF"
                     /locus_tag="AC16Y7_07275"
     CDS             6941..7474
                     /gene="rplF"
                     /locus_tag="AC16Y7_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR5709218.1"
                     /translation="MSRIGKRPVAIPGGVTAAVDGQEIKVKGPKGELRHVLADQIMAK
                     TGDDGIEIAMRADSNEARAMWGMSRTLVANLVTGVTEGFSKTLEITGVGYRAAVKGQT
                     LQLQLGFSHDVNYAIPQGIEVKCPKPTEILVSGIDKQLVGQVAAEIRRYRPPEPYKGK
                     GVRYAGEYILRKEGKKK"
     gene            7487..7843
                     /gene="rplR"
                     /locus_tag="AC16Y7_07280"
     CDS             7487..7843
                     /gene="rplR"
                     /locus_tag="AC16Y7_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR5709219.1"
                     /translation="MSALNNTFARRTRRVRHSLKRAANGRARLSVFRSSKHIYAQVID
                     DSAGKTVAAASSLDKDFKLEKGADTSAAEAVGKRVAERAVKAGVKDVVFDRGGYLYHG
                     RVKALADAAREAGLNF"
     gene            7865..8443
                     /gene="rpsE"
                     /locus_tag="AC16Y7_07285"
     CDS             7865..8443
                     /gene="rpsE"
                     /locus_tag="AC16Y7_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR5709220.1"
                     /translation="MARGPQSRGPQGRDRDDRDSEFVDKLVHINRVAKVVKGGRRFGF
                     AALVVVGDQKGRVGFGHGKAREVPEAIRKATESAKRNLVKVPLREGRTLHHDVRGQHG
                     AGKVILRAAPPGTGIIAGGPMRAVFETLGIQDVVAKSIGSSNPYNMVRATFDALRHED
                     SPRGVAARRGKKVSEIVSRRRDQAGDGTEAQA"
     gene            8469..8672
                     /gene="rpmD"
                     /locus_tag="AC16Y7_07290"
     CDS             8469..8672
                     /gene="rpmD"
                     /locus_tag="AC16Y7_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009469221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR5709221.1"
                     /translation="MAEKQKTIVVEQIGSPIRRPGSQRATLIGLGLNKMHKRRTLVDT
                     PQVRGMVAKVSHLVRIVEDEAGA"
     gene            8685..9299
                     /gene="rplO"
                     /locus_tag="AC16Y7_07295"
     CDS             8685..9299
                     /gene="rplO"
                     /locus_tag="AC16Y7_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009341042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR5709222.1"
                     /translation="MRLNEISDRPGATKPKKRLGRGLGSGLGKTSGRGQKGQKSRSGV
                     AIKGFEGGQMPLHRRLPKRGFKNIFAKDLNEVNVGRVQAAIDAKKLDAKAPITVQALL
                     DAGVIRRVRDGVRLLGNGEIKTKATIEVTGASSGAVKAVEAAGGTVTLTPMPRRAPKP
                     DGEGKPKKDSKTAAKSAAPKKTEGAPAKKPAAKKKAPAKKDTSE"
     gene            9356..10690
                     /gene="secY"
                     /locus_tag="AC16Y7_07300"
     CDS             9356..10690
                     /gene="secY"
                     /locus_tag="AC16Y7_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR5709223.1"
                     /translation="MVSAAEQLVSNLNFAAFAKADELKKRIWFTLGALIIYRLGTYIP
                     IPGINPEALAEVFRQQQSGILGMFNMFSGGAVGRMAIFALNIMPYISASIIIQLLTTV
                     SPHLEQLKKEGEQGRRQINQYTRYGTVLLAALQGYGIAVGLEGTGNVVTDPGWFFRIT
                     TVITLVGGTVFLMWLGEQITARGVGNGISLIIFAGIVAELPSALVGTLELGKQGALST
                     ALIIALFVMAVAVIAFIVFMERAQRRLLVQYPKRQVGNKMFQGDASHLPLKLNTAGVI
                     PPIFASSLLLLPITVANFSAGQGPEWLNTITALLGRGQPLYLLIYISLIIFFAFFYTA
                     VVFNPKDTAENLKKYGGFLPGIRPGERTAEYIDYVLTRITVVGALYLAAVCVLPELLI
                     SNWGVPFYFGGTSLLIVVSVTMDTVAQVQSHLLAHQYEGLIKKAKLRGARRR"
     gene            10687..11337
                     /locus_tag="AC16Y7_07305"
     CDS             10687..11337
                     /locus_tag="AC16Y7_07305"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR5709224.1"
                     /translation="MILVLIGPPGAGKGTQAKRLEDLRGLVQLSTGDMLRAAVAAGTE
                     LGRQAGDLMEHGQLVPDDLVIGLIAERLDANKDAKGFILDGFPRTIGQAEALDRLLET
                     RGAQVDRTVVIDVDDDALVVRITGRFTCATCGEGYHDLFKKPKEEGTCDKCGGTEFKR
                     RPDDTEEVVRSRLKAYHAQTEPLVGYYGGQDKVRTVNGMADIEAVQKEIDLALDAA"
     gene            11531..11899
                     /gene="rpsM"
                     /locus_tag="AC16Y7_07310"
     CDS             11531..11899
                     /gene="rpsM"
                     /locus_tag="AC16Y7_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011651425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HIR5709225.1"
                     /translation="MARIAGVNIPTNKRVEIALTYIHGIGDTFAKDICTKVSIPTERR
                     VNELTDAEVIQIREVIDRDYMVEGDLRREVAMNIKRLMDLGCYRGLRHRRGLPVHGQR
                     THTNARTRKGPARPIAGKKK"
     gene            11975..12364
                     /gene="rpsK"
                     /locus_tag="AC16Y7_07315"
     CDS             11975..12364
                     /gene="rpsK"
                     /locus_tag="AC16Y7_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007603045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HIR5709226.1"
                     /translation="MAKEKGRVRRREKKNITSGVAHVHASFNNTMITITDAQGNAISW
                     SSAGTMGFKGSRKSTPYAAQVAAEDAGKKASEHGMKNLEIEVRGPGSGRESALRALQA
                     AGFNIISIRDVTPIPHTGCRPRKRRRV"
     gene            12403..13473
                     /locus_tag="AC16Y7_07320"
     CDS             12403..13473
                     /locus_tag="AC16Y7_07320"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR5709227.1"
                     /translation="MRKQEPTAPLNDQERATPVLQKNWQDLIKPTKLNIVPGRDPSRY
                     ATIGAEPLERGFGLTLGNALRRVLLSSLQGAAITSVHIDGVLHEFSSIPGVREDVTDI
                     VLNIKEIALSQHSEGVKRMVLQKEGPGIATAGDIQTGSEVEILNPEHVICTLDEGTAI
                     HMEFTSSSGKGYVPAERNRPDDAPIGFIPVDSLFSPVRKVSYRVENTREGQILDYDKL
                     TMEIETDGSVRPEDSLALAARVVQDQLSVFVNFEEPKKEQTFERQPELVFNAALLKKV
                     DELELSVRSANCLKNDNIVYIGDLIQKTEAEMLRTPNFGRKSLNEIKEVLASMGLHLG
                     MDVPDWPPENIEELAKRFEDHY"
     gene            13525..13953
                     /gene="rplQ"
                     /locus_tag="AC16Y7_07325"
     CDS             13525..13953
                     /gene="rplQ"
                     /locus_tag="AC16Y7_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HIR5709228.1"
                     /translation="MRHRKAGRKLNRTATHRSALLSSLACALIKHEQIATTLPKAKEL
                     RRVADRLITLAKRGDLHARRLAFARIRDDAMVSKLFETLGPRYADRPGGYTRVLKAGF
                     RYGDSAPMAVIELVDRDEDAKGLDSGPTQEVAEEGEAAAA"
     gene            complement(14032..14580)
                     /locus_tag="AC16Y7_07330"
     CDS             complement(14032..14580)
                     /locus_tag="AC16Y7_07330"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5709229.1"
                     /translation="MRVFKLTLVALAATFAWSAAAEAVEVKKRREAPGTPAEVWAVVG
                     DFCAIKDWHEAVADCEQIEEGSDTYRILTLGDGGKIKEKLTETDDTSYSYEIIEGPLP
                     VKNYSATLEVEADDEPDRVEIEWEAEFDADGVEDDEAKKIIVGIFNTGVSAIKTKAIE
                     AYDKREAAAGRPVTTGRDRSKF"
     gene            14785..16218
                     /locus_tag="AC16Y7_07335"
     CDS             14785..16218
                     /locus_tag="AC16Y7_07335"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HIR5709230.1"
                     /translation="MTLLEKLSGKIGILCLAGASLLLSVPGTLAQERAVPKNQAAMKQ
                     SFSPVVKQAAPAVVNVYVRHRVKQSVSPFFRDPFFRRFFGDKFGVPRERIQNSLGSGV
                     LVRGEGVVVTNYHVIRGGEKSEVTVALSDGREFPAEIILKDEKTDLAVLRLDGEGAKF
                     PSIKFANSDTLEVGDLVLAIGDPFGVGQTVTSGIVSGLARTKVGFSDYQFFIQTDAAI
                     NPGNSGGALVDMDGRLVGINTAIFSKSGGSHGIGFAIPSNMVRLVVQSALKGGKLQRP
                     WFGASLQPLNADLAESIGLDRPSGAMVTSVHKNGPARGAGLQVGDVVVSIDDKTVKDP
                     EALQYRFVTKGVGGKAKLGVYRNRKLMNATISLIAPIEDPPRDIRELEGRHPFAGSKV
                     ANLSPAVAHEIGMDDDAKQGVVVLDVTRGSNAGRLGLRRGDIVVGINKEKVSSVEQLS
                     LLLQLDTGGWRLSVERGGKVFNLAIQG"
     gene            16221..17546
                     /locus_tag="AC16Y7_07340"
     CDS             16221..17546
                     /locus_tag="AC16Y7_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR5709231.1"
                     /translation="MGEDAATLFEAAGLGDAAPRPLADRLRPKSLDEVIGQDHLLGDG
                     MLGRMVRVGRLSSVIFWGPPGTGKTTVARLLAEVTDLHFEQLSAIQSGVADLRKVFDT
                     ARARRQEGRGTLLFIDEIHRFNRSQQDAFLPHMEDGTIVLVGATTENPSFELNAALLS
                     RASVLTFKRLDDDALEALLARAETHEGRKLPTTDDARTALISMADGDGRGLINLAEDV
                     FLAVGKDAKLVLDREALVELVQRRAPIYDKGQDGHYNLISALHKSVRGSDPDAALYWL
                     ARMLVAGEDPLYIARRLVRMAVEDIGMADPAGLVQANAAKDAYDFLGSPEGELALAQA
                     TVYLACAPKSNAAYVAYKAAMGVAKESGSLLPPKHILNAPTRLMKQEGYGRDYAYDHN
                     ETEAFSGQDYFPEEMERPDFYAPTGRGFEREIRERLERWGEIRAKRAQR"
     gene            17565..17939
                     /gene="crcB"
                     /locus_tag="AC16Y7_07345"
     CDS             17565..17939
                     /gene="crcB"
                     /locus_tag="AC16Y7_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HIR5709232.1"
                     /translation="MQTLLCVAAGGALGASGRYLVGIASGRLFGLDFPWGTLIVNVLG
                     CLVMGLLIEAMALRWTVHNDLRAFLAVGLLGGFTTFSAFSADFGLLFERDAYLSAGLY
                     LSASVGLSIAAFFAGLAIVRAL"
     gene            17942..18946
                     /locus_tag="AC16Y7_07350"
     CDS             17942..18946
                     /locus_tag="AC16Y7_07350"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR5709233.1"
                     /translation="MPPVQTRHVTEDEDGMRLDRWFKAHYASLPHSRLEKLLRTGQVR
                     VDGGRVKASTRLSAGQSVRVPPLPDAPPPPGEKRTLSKADRAFLKEITLYEDDDLLIL
                     NKPSGIAVQGGTKTTRHIDRLLEGLSDRPETRPRLVHRLDRDTSGVLVVAKRRSVAAK
                     LGRAFQTRSVRKIYWALVHGVPKPPQGKIDAALVKAATPDGERVRKARAGEQAQAQSA
                     VTHYAVIDRAGQQVALMSLKPVTGRQHQLRAHMAMAGHPMLGDEKYPSDIDLPDGIER
                     KLHLHARRIAFPHPSGEGVVDVTAPLPPHMEQAFSAFGFTVKGGEVEEAAFDRDACTR
                     "
     gene            19038..19433
                     /locus_tag="AC16Y7_07355"
     CDS             19038..19433
                     /locus_tag="AC16Y7_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011425088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HIR5709234.1"
                     /translation="MPPKTSWFTRFAQLAAHITGRPRCFFLAVSVIVLWALAGPLFDY
                     SDTWQLVINTSTTIVTFLMVFVIQNTQNRDTAAMHIKIDELIRVTEKARNVLLDLEEL
                     DENKLEAMRRDYERLAEEGRARAEAVELT"
     gene            19436..20188
                     /locus_tag="AC16Y7_07360"
     CDS             19436..20188
                     /locus_tag="AC16Y7_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family chaperone protein"
                     /protein_id="HIR5709235.1"
                     /translation="MAEKDTNAPQNGASQKSSLPKRFYKDVTVADEDGHAAILLDGKP
                     VRTPGKAQLALPSRALAEAIAEEWRAQGKHIDPATMPLTKLANSAIDGVVGQADAVID
                     DILAHARSDHLCYRASGPEKLLAAQGQHWDPVIAWAKEVLGAPFNLTEGVVHVAQPDA
                     SIEALKGEVEKLDAFGLAAVHVMTTLTGSALLALAVALKRLTPEEAWAAAHVDENFQI
                     SQWGEDEEAKARREARKRDFDAAARALELSES"
     gene            20310..21842
                     /locus_tag="AC16Y7_07365"
     CDS             20310..21842
                     /locus_tag="AC16Y7_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR5709236.1"
                     /translation="MKEVLEELEDRRAKARMGGGKRRIDAQHKRGKLTARERLQLLLD
                     DGSFEEFDMYVEHRCADFGMAETKVPGDGVVTGWGTINGRVVYVIAKDFTVFGGSLSE
                     AHANKMIKIQDMALRNRAPVIGLFDAGGARIQEGVAALGGYGEVFLRNVQASGVIPQI
                     SVIMGPCAGGDVYSPAMTDFIFMVKDTSYMFVTGPDVVKTVTKEEVTAEELGGAVVHT
                     TKSSIADGAYENDVEALLQMRRLMDYLPSSNLSGVPELPTRDPWNRPEPSLDTLIPDN
                     PNKPYDIKELIHKVVDEGDFFEIQETYAGNIVTGFARMEGRTVGIVANQPMVLAGVLD
                     SDASRKAARFVRFCDCFSIPIVTFVDVPGFLPGTDQEYGGLIKHGAKLLFAFTEATVP
                     KVTVITRKAYGGAYDVMSSKHIRGDVNYAWPSAEIAVMGAKGAVEILYRADLGDPEKV
                     QKRIDDYQERFANPFVAAERGYIDEVIAPRGTRARICRALQMLRNKHVENPWKKHDNI
                     PL"
     gene            22098..22307
                     /locus_tag="AC16Y7_07370"
     CDS             22098..22307
                     /locus_tag="AC16Y7_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR5709237.1"
                     /translation="MIKGTVKFYNEQKGFGFIEPEDGGKDVFVHATALERAGMGSLAE
                     GQKVSFDTEVDSRSGKTAVARIEAA"
     gene            complement(22403..22660)
                     /locus_tag="AC16Y7_07375"
     CDS             complement(22403..22660)
                     /locus_tag="AC16Y7_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709238.1"
                     /translation="MIRSEGGSVRDTWLGLGKMAWTISDTLGVSKKKGKGDKPKKGSK
                     AAADAKRSAMDGALKNYGLAVNYTASAQRTLNGPLCARIGP"
     gene            complement(22657..22941)
                     /locus_tag="AC16Y7_07380"
     CDS             complement(22657..22941)
                     /locus_tag="AC16Y7_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709239.1"
                     /translation="MYPETSFECQKSQGTSSAAFLTGDQWGSWDGRLVVGIMGIGFGG
                     TPVGQRIDVIRLSEDGKSAEEVVTLPFPSAAGRFRSARQRPVPWRWRRLE"
     gene            23028..23351
                     /locus_tag="AC16Y7_07385"
     CDS             23028..23351
                     /locus_tag="AC16Y7_07385"
                     /inference="COORDINATES: protein motif:HMM:NF021390.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HIR5709240.1"
                     /translation="MTNRVMTLAGVAIAGLLAGPAFAEGPVETTVTYECAGGKSIVAM
                     YTSDEVALTFHDGRKLALPQVISGSGTRYANADESIQFWSKGKTAFVTEGSDDNVTYK
                     DCVAD"
     gene            23481..23858
                     /locus_tag="AC16Y7_07390"
     CDS             23481..23858
                     /locus_tag="AC16Y7_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709241.1"
                     /translation="MRLGAWGWSLAVCAVLLSGSAFAVSLSSSLKNYYAEFEIVLECE
                     VKEHLSTAEADAAEAAIDKIEDYFLAKDSSINTESLQKKAVADKNEGLRIMARSGGGL
                     TPYCRQSLRELLTKAKEIGPAAQ"
     gene            23967..24251
                     /locus_tag="AC16Y7_07395"
     CDS             23967..24251
                     /locus_tag="AC16Y7_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709242.1"
                     /translation="MRPRGLSAFVFVAALLAMVVPSVAQYSKAIKGACRNDYKRFCST
                     YAVDDPALRKCMDKAGPSLSQRCVATLVNSGLVSYQRATQRWGHKLEKVQ"
     gene            24500..24904
                     /gene="lanM"
                     /locus_tag="AC16Y7_07400"
     CDS             24500..24904
                     /gene="lanM"
                     /locus_tag="AC16Y7_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanmodulin LanM"
                     /protein_id="HIR5709243.1"
                     /translation="MHKFALTFAIAISTFFLAGSPASAVDSATALRVLDPDNDGTIDM
                     REALRGAKRVFKAINPDRDGTLDAAELRGRLSAAGLRAADPDDDGTLSWREYKRLVKA
                     LFVLANPDDDGTIDLRELRSPAGRRLLKLIYL"
     gene            25077..25784
                     /locus_tag="AC16Y7_07405"
     CDS             25077..25784
                     /locus_tag="AC16Y7_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIR5709244.1"
                     /translation="MTDLYKTLGVKRTAAPKDIHKAYRKKAKSAHPDSGGSVEAFNTL
                     VLAYSVLSDKGRRTRYDETGEIDPPRPDNFDGGAIEIIAEKLGLVIHAEQDLATMDVG
                     RLIEQAIRDDIAARKASVTHQQRAGERLAALRSRTKRKAEGDANLVARVLDWHELAIR
                     NHIKKHEESIRFLERALEILHGYSFTDDLAAAQAEEVAGALNDILVALDELAAVLNTT
                     PSRAEAMPQGAGPTAFG"
     gene            25854..27560
                     /locus_tag="AC16Y7_07410"
     CDS             25854..27560
                     /locus_tag="AC16Y7_07410"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase scaffold domain-containing
                     protein"
                     /protein_id="HIR5709245.1"
                     /translation="MRRYFLIAMTCCMALAACLGALAAARAETQTVPPLRKLPWESDT
                     AATQPPEPAVSPSVSSPTPAPEAASETDSEEETDTAKDEPDEGEPEEVAEAADLPPVP
                     AHPTVTIIREKLTDPKVTKDAADEDVAGLQAFYRAWGEPPLWVTETGLSTKGKAAIRE
                     IGKADDWGLKADDFTVPEADVQLATAEAQALAQITLDLAILKYARLASGGEHVPSEIS
                     DLFDQSPPFRDPKIVMADIAASDSPDIYLQTLHPAHEQFDRLRDALIEARKADEPKET
                     DIRRLLMNMERWRWMPEELGPLYVWLNTPEYMLYVVKDGETVFKDKTLVGTIGNPTPV
                     FSADMETIVFNPDWIAPPTVLKEKFWPALKRKYYDILKSNKISVSYRGKRIDPKKIDW
                     QRTNIHKFTFRQKAGPKNVLGKAKFLYPNKHVVYMHDTLPPRRKIFKEDKRDIGYGCV
                     RMSKPQAFAAVMLAEDQELPQSDVKKFWNKSVNKAVSMEKKPPVHTTYFTAVVDEEGK
                     VSTFKDLYGLDRMQAKAVFGSVDGFPKPPPEKKRLSQSVAASSRDSNTGAGSTNSLGF
                     GD"
     gene            complement(27570..30746)
                     /locus_tag="AC16Y7_07415"
     CDS             complement(27570..30746)
                     /locus_tag="AC16Y7_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5709246.1"
                     /translation="MNGIIDAAFARTRTVALAFFMIIVMGAVAYTTIPKEAEPDIQIP
                     IIYVSMTYEGISPEDSERLLIRPMEKELKSIEGIKEMRSVAKEGYGSVTLEFDAGFNP
                     DTALEDVREKVDIARAELPPETDEPRVNEVNVALFPVITVGLSGNVPERTLVRIARDL
                     RDKIEALGGVLDVDIGGDREELLEIVVDPMVLETYNVSFADVLTTVRRNNRLVAAGAL
                     ESAAGRMVLKVPGVIEEVQDILNLPIKVVGTTVVKFSDVASIRRTFKDPQGFARVGGN
                     PALALEVKKRVGANIIETIEEVRAVVKEEQASWPPGVQVSFMQDKSKQIRDILSDLQN
                     NVLAAIALVMIVIIAAIGVRSAILVGLAIPGSFLAGILAISLMGLTLNIVVLFSLILV
                     VGMLVDGAIVTTELADRKMQEGKDRKQAYSEAAKRMAWPIAASTATTLAVFLPLLFWP
                     GIVGEFMKFLPITVLVTLAASLTMALVFIPVLGGIFGKTDKGDGSLLQTIRAAEDGDL
                     NKIGGAAGAYLNTLKRLLAHPAKVLCVAVALLIGTYTAFGMFGRGVEFFPDVDQDIAQ
                     VQVRARGDLSVTEMDAVVRKVETRLLGMPHFKTVYAKTIGSGAPPSQTGSAEDVIGVV
                     QFELTNWRTRPRASDILKEVRTRTADIPGVILEVRKQKGGPTAGKPVELHVTSRDTEK
                     MPDVVTKLRAEMNKIGGFVDEQDSRPLPGIEWRLKVDREKAARNGADVAVLGEAVSML
                     TNGIFVAEYRPDDADEEVDIRLRLPLAERNLEQLNRLRVPTDKGQVPIGNFVSFEPAQ
                     KIGNLNRTDGRRVLEIQADVAEGLLVDDQVRKLKTAVAETDLDPTVTVRFKGQDADQR
                     EAGNFLIKAFFIAIFMMVAILVTQFNSFYQTALVLSAIVFSTAGVMIGLLATDQPFGI
                     VMCGIGIIALAGIVVNNNIVLIDTYNDLRSRGQPAKEAILRTAAQRMRPVLLTSITTV
                     LGLMPMVFSLSIDIIGQDVSVGAPSTQWWTQLSSSIAGGLAFATMLTLVLTPCLLMLG
                     DNVAVWWHGRAGRKAQRVSRKGRPQAAS"
     gene            complement(30743..31888)
                     /locus_tag="AC16Y7_07420"
     CDS             complement(30743..31888)
                     /locus_tag="AC16Y7_07420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5709247.1"
                     /translation="MKKSYIIAAVLTLALTAWVLGGYFLRTVGKEADGTTPVADAAPT
                     MRVAVRTQEAKPVSQIVVAQGQAEPNRTVTVRAETLGQIAEILADEGSTVAAGDVIAR
                     LEPNAREARIERAKARLREQESAYEAAQALGKKGYQTQRQSDQIYSSLQTAKHELEVA
                     MIEQERLDIKAPFEGEVLTVPIELGAYVDVNGEVATIVDNHPLVVSVQITQQDITDLK
                     VGQKATVTFATGQKGEGKIRYIAARADEGTRTFRVEIELPNPDGAIPSGISAEARIPT
                     GSVMAQFVSPADLSLNEAGKLGVKTVGKDSRVVFHEASIVMSDASGAWVIGLPPQARI
                     ITLGHGYVQIGEQVQVAEEQGSTGMDGSKEAAARLTTRRVDEPTAAQ"
     gene            complement(31935..32441)
                     /locus_tag="AC16Y7_07425"
     CDS             complement(31935..32441)
                     /locus_tag="AC16Y7_07425"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HIR5709248.1"
                     /translation="MADKSEDVVIEFVEQMGLILQGEGLPRIAGRIMGLMIMHGGPFS
                     LTELAERLSVSRASISTNTRLLEELGVIERTATPGDRQDYFRLSRQPYARMLRGVVRR
                     MRRAREVVEGTQESLPEDMSETHERLEELDAFYEALIESFLNVIDEWDAERQGSARHK
                     RASARPSA"
     gene            32655..34673
                     /locus_tag="AC16Y7_07430"
     CDS             32655..34673
                     /locus_tag="AC16Y7_07430"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR5709249.1"
                     /translation="MFKKLLIANRGEIACRIIRTAKRLGIETVAVYSEADRDALHVDM
                     ADEAVGIGPAAAAQSYLVMDKIIAAAKETGADAIHPGYGFLSENAAFAKALKKAGIAF
                     VGPNPRAIEAMGDKIESKKLAAKAKVSTVPGHLGVIEDAKAAVKIADDIGYPVMIKAS
                     AGGGGKGMRIAYSSDEVAEGFASSQSEAKSSFGDDRVFIEKYIEEPRHIEIQVLGDKH
                     GNVIHLGERECSIQRRNQKVIEEAPSPFLDEKTREAMGAEAVALSKAVDYDTAGTVEF
                     IVDKDRNFYFLEMNTRLQVEHPVTELVTGLDLVEQMLKVAAGEKLSIKQKDVTLTGSA
                     IESRIYAEDPVRNFLPSTGRLVRFRPPELGTEDGVTIRLDTGVEEGGEISVYYDPMIA
                     KLVTHAPTRIEAIEEMGWALDAFAIDGFRHNIPFLAVLMRNERWREGRLSTKFIDEEF
                     PDGFHPPVPEGHVRDVLAAVAASVDYLSNNRRRMITQQMSGEPVQFTDSRVVDLGDQP
                     VSVGVSGRDYPMTVTVGGEGKGEKAREIVLDSPWWFGDPVWEGTVDDEEVAVQVRPIL
                     NGVHLTYQGVEVPVRVYTERESGYLALMPVKEKPDMSKYLLCPMPGLVVSLAVEEGQD
                     VSAGDTLCVVEAMKMENVLKAERDGTVSKILAKPGDSLNVDAVILEFA"
BASE COUNT         6854 a        10310 c        11221 g         6325 t
ORIGIN      
        1 gccggcgtgg atgtcgagat caagctctaa cggctgcagg acgaggcaag gaacaagttc
       61 gatgcgatca ggtgtcatcg cgcaaaaggt cggcatgacc aggatcttca ccgacgccgg
      121 ggaacacatc ccggtgacgg tgctgcgtct ggaaaattgt caggtcgtgg cccagcgcac
      181 tgccgagaag aacggctata cggccgtgca acttggggcc ggccggccca aggtcaagcg
      241 tctgacgcgc gccgagcgcg gcgggttcgc cgtggccaat gtcgagccga agcggaaggt
      301 agcggagttc cgcgtgagcc cggagaacat gatcgacgtg ggcgcggaga tcaccgccga
      361 ccacttcgtc gagggccagt tcgtggacgc atccggcacc tcgatcggta agggatttgc
      421 cggcggcatg aagcggcacg gctttggcgg cctcagggcc agtcacggcg tgtcgatcaa
      481 tcaccgtagc cttggttcga ccggtcagtg tcaggatccg ggcaaggtgt tcaaaggtaa
      541 gaaaatggcc ggccagatgg gctccgtcgc cgtcaccaca cagaacctgc gggtggtgag
      601 aacggacgtc gatcgcggac tgatcatgat ccgcggtgcg gttccaggcg ccaagggcgg
      661 ctgggtgctg ctgcgcgatg ccgtgaagcg tgctttgccg gatgcggcgc caaagcccgg
      721 cgcgttccgg aaaggcgcgg acgatcagaa gccggcgaag gaagcaaagt tggaaacgaa
      781 gccagaggcc gcgcctgagg cgccggtgcc tgaggccaag cccgaagagg gcggggataa
      841 agcgtgatga aggcggacgt aacaacgctc gacgccaaga aggcgggcac gattgagctc
      901 tcggacgacg tgttcgggct ggagccgcgt gccgacctgc tgcatcgcat ggtgcgctac
      961 cagcttgccg cccgacgcag aggcacggtc gcggtcaagg accgttcgga gatcgtcggg
     1021 acgacagcca agatgtttcg ccagaagggc acgggccggg cgcgccacgg caacgccaag
     1081 ccgacgatct tccgtggcgg cggtaaggca ttcggtccga agccgcgtag ccatgacatc
     1141 gagctgccga agaaagtgcg cgcgctggcg cttcgccacg cgctgtcgtc caaggctaag
     1201 acggacgcgc tggtggtgat cgattcctgc gagatgaaag atccgaagac caaggcgctg
     1261 aaggcacggt tcgacaagct cggcgtgagc tcggcgctcg tcgtcgacgg cgcggaggtg
     1321 caggacggct tcgcgcgtgc cgcgcgcaac attccgcaca tcgatgtgct gccggtgcag
     1381 ggcatcaatg tctacgacat ccttcggcac gatacgctcg tgctgacgaa agccgcgctc
     1441 gaagcgctgg aggcacggtt caaatgagtg cgcttcaagc atatgacgtg atcctgtcac
     1501 cggtgatcag cgagaagtcg agcgaggcgt cggagtcgaa ccatgtggtg ttccgcgtgc
     1561 gcctcgatgc gaccaagcct cagatcaaga aggcggtcga gaagctgttc ggcgtgaagg
     1621 tggtcgcggt gaatacactg acccgtaagg gcaagaccaa gcggttccgc ggcatcgaag
     1681 gccgccagaa agatgtgaag aaggccgtcg tcaagctcgc cgaaggcgag tcgattgacg
     1741 tgacgacagg gatttaacga gatggcgctg aagaacttca aaccaacatc gccgagccag
     1801 cgtcagctgg tgctcgtcga ccgcagccat ctgtggaagg gcaagccggt caagtcgctg
     1861 accgagggct tgaacaagtc cggtggccgc aacaacacgg gacgggtctc gatctggcat
     1921 cgtggtggcg ggcacaagcg ctcataccgg ctggtcgact tcaagcggac caagcgcggc
     1981 atgccggcga cggtcgagcg gatcgaatac gacccgaacc gcacggcgtt cattgcgctg
     2041 atcaagtacg aagacggcga tctcgcatac attttggcgc cgcagcgcct ggcgccgggc
     2101 gacacggtga ttgccgacga caaggcagac gtgaagccgg gcaacgccat gcctttggcc
     2161 agcatgccga tcggcaccat cgtccacaac gtggagatga aggtgggcaa gggtggccag
     2221 atcgcgcgag cggctggtag ctacgtccag ctcgtgggcc gcgacgctgg ctatgcgatt
     2281 ttgcgcctca actccggcga gacacgcatg gtgcctgccg gctgcatggc tacagtgggt
     2341 gccgtgtcca acccggacaa ttccaacaca tcgttcgcca aggccggacg catgcggtgg
     2401 aaggggcgca agcctgtggt ccgcggcgtg gccatgaacc cgattgatca tccgcatggt
     2461 ggtggtgaag ggcgtacctc cggtggccgg catcctgtca ccccgtgggg taagtccacc
     2521 aagggcaagc gcacgcgttc ccaaaaggga agcgacaagt tcattctgcg cagccgccat
     2581 cagaaaaaga agaagaaggg ataagacgtg gcacgttcga tttggaaggg tccgttcgtc
     2641 gacggctatc tcttgaagaa ggccgagaag gcgcgtcaga gcggttccaa cgccgtgatc
     2701 aagatgtggt cgcgccgttc gaccatcttg ccgcagttcg tgggactcac cttcggtgtc
     2761 cacaatgggc ataagcacgt tccggtgctc gtaaccgagg acatggtcgg ccacaagttt
     2821 ggcgagttcg cgccgacgcg cacctatcac ggtcataccg ctgaccgtaa agcgaagaga
     2881 gcttaattat gggcaagccc caaagagagc gcacgctcaa agacaatgag gcgaaggccg
     2941 tgacccggct tattcgtgtg agcccgcaga agctcaacct ggttgcccag cttatccggg
     3001 gcaagaaggt cgaccgtgcg cttgccgacc tcaccttctc gcgcaagcgc atcgccaagg
     3061 atgtgaagaa gacgctggag tcggccatcg ccaacgccga gaacaatcat gacctcgacg
     3121 ttgatgcgct gatcgtgtcg gaggcctatg tgggcaagaa tatcgttatg aagcggcttc
     3181 gggcgcgtgc acgtggccgg tccgctcgga ttttgaaacc gttttcgcag atgaccgtgg
     3241 tcgtgcgcca ggtcgaggag tccgcctgat gggacagaag gtcaatccga tcgggctaag
     3301 gctcggcgtc aatcgtactt gggactcccg ctggttcgcc agcggaggtg agtacgcgca
     3361 gctgctccac gaggacttga agatgcgtga gcatattctc aactcgcgca agcaggctgg
     3421 catttccaag gtggtcatcg agcgcccgca caagaagtgc cgcgtgacgg tctacacggc
     3481 gcgccctggc attttgattg gcaagaaggg cgccgacatc gagaagcttc gccgcgaact
     3541 ggcgacgatg acggattccg acgtgcatct gaatatcgtc gaggtgcgca agcccgaggt
     3601 tgacgccacg ctggtggctg agggcatcgc gcagcagctt gagcgccgcg tggcattccg
     3661 ccgggcgatg aagcgggcgg tgcagtcggc catccgcatg ggtgcgcttg gcatccgtat
     3721 caattgtggg ggccggctcg gtggcgcgga gattgcgcgt acggagtggt accgcgaggg
     3781 ccgtgtgccg ctgcacacgc tgcgcgcgaa tatcgactac ggcacggcgc tggggcgcac
     3841 ggcttacgga atcatcggca tcaaggtttg gatcttcaag ggcgagatca tggagcacga
     3901 tcccatggcc caggagaacc gggcgattga ggcgcaagag ggcgggcgtt ccacgggccg
     3961 ccgtgaaccc gcttcggcgc gaggctaagg gcaaggctta gagttatgct gcaaccgaaa
     4021 cgcaccaagt accgcaagat gcagaaaggg cgcatcaaag gcgccgccaa gggcgcgacc
     4081 gcgctcaact tcggcacctt cgggttgaag gcgcaagagc cggcccgtgt caccgcgcgg
     4141 cagatcgagg cggcgcgacg tgccatgacg cgtcagatga agcgcgccgg ccgtgtgtgg
     4201 atccgcgtgt tcccggacgt gccggtgtcg aagaagccga ccgaagtgcg catgggtaag
     4261 ggtaagggta cgcctgagtt ctgggcggcc aaggtcaagc ccgggcgcat catgttcgag
     4321 attgacggtg tgggcgagtc cgtcgcccgt gaagcgttgc ggcttgctgc cgcgaagttg
     4381 ccgatcaaga cgcgcgttgt ggcgcgcacc gagggctaag cgatgaaagc cagtcaggtg
     4441 cacgacatga cgatcgatca gctcaacgac gagctggtca aattgaagaa agagcagttc
     4501 aacctgcgtt ttcaggcggc ctcgggtcag ctggagaaca cggcgcgggt tcgacaggtc
     4561 aggcgcgaca ttgcgcgcgt ccagactgtg gcgcgcgaaa gagacgcagc agcgaaggtt
     4621 aaagggtaag aacgatgccg aaacgcgttt tgacaggggt cgtggtgagc gacaagaacg
     4681 acaagacggt taccgtcaag atcgagcgcc gcttcacgca ccctctgttt aagaaggtgg
     4741 tgcgccggac gaagaactat cacgctcacg atgagaagaa tgaattcaag gtgggcgaga
     4801 aggtttcgat cgaagagtgc gcgccgatct cgaggcacaa acgctgggtg gtcctgccgc
     4861 gtgagcaggc aacggcgtaa ggaatgaata gaggcccgca agtggcctca cgttaagggc
     4921 gaatgccatg attcagatgc agaccaatct cgatgttgcc gacaactctg gcgccaagcg
     4981 cgttcagtgc atcaaggtgc tcggcggctc gaagcgcaaa tacgcgtcga tcggcgacac
     5041 catcgtggtc agcgtcaaag aggccatccc gcgcggccgc gtgaagaagg gccaggtgat
     5101 gaaggccgtg atcgtgcgta ccgcgtccgg tgtgcgccgt ccggacggct cgctcatccg
     5161 ctttgatcgc aacgccgcgg tcctggtcac gccgcaaggc gagccggtgg gtacacgtat
     5221 cttcgggccg gtgacgcgtg agttgagggc gaagaagcat atgaagatcg tgtctctcgc
     5281 gccggaggtt ctttgatcat gcgaaatctg aagatcaaga agggtgacca agtcgtggtc
     5341 atcacgggca aagacaaggg caagcacggc gaagtcctga aggtgatgcc gtcggagaac
     5401 cgcgccatcg tcaagggtgt gtcgatggtg cgccgtcatc agcggcaaac cgcgaaccaa
     5461 gagggcggca tcatcaccaa ggaagccccg atcgagattt cgaacatcgc gctggaggac
     5521 ccgaaggacg ggaagcccac gcgcgccggc tacaagtttc taaaggacgg gcgcaaggtt
     5581 cgatttgcca ggcgctcggg cgaggtcatc gatggctgag caggtttacg ttccgcggct
     5641 gaagctgcag taccaagacg ttatcaaggg cgatctctcc aaagagttca gctacccgaa
     5701 cgtcatgcag gttccgcgcc tggagaaggt ggtgctgaac atgggcgttg gcgatgccgt
     5761 cagcgattcc aagaaggtga aggccgccgc ggccgatctc ggcctgattg ccggtcagcg
     5821 tcctgtcatc accaaggcgc gcaagtcgat cgccggcttc aagctgcgcg aaggcatgcc
     5881 ggtcggggcc aaggtgacct tgcgcaagga ccgtatgtac gagttcctag atcggctcgt
     5941 gaatatcgcg ctgccgcggg tccgggactt ccgtgggctt agtccgaaga gcttcgacgg
     6001 aagaggcaat tattcgatgg gcctgaagga gcatatcgtc tttcccgaga tcgattacga
     6061 caaggtggaa gatgtgcttg gcatggacat catcgtttgc acgaccgcgc agaccgacga
     6121 cgaggcgcgc gcgttgctga agggtttgaa ctttccgttc cgcagctagg tgttcccggc
     6181 tgcgacagga ggactgaatg gctaagacga gcatgattga gcgagacaag aagcggcgcc
     6241 gcctcgccaa aaagtttgca taccggcggg cgcggttgaa ggaaatcgct gccgaccgct
     6301 catttccggc ggaggagcgc ttcgcggcgc ggttgaagct agctgagctg ccgcgtaatt
     6361 cctccccgac gcgcattcgt aaccgttgtg atctgtccgg gcgcccgcgt ggctactacc
     6421 ggaagcttcg catgtcgcgc attgctttgc gcgatctggg aagcaccgga atgatcccgg
     6481 gcatggtcaa gtcgagctgg taaggacgat cgatgaacgt caacgaccca ctaggcgata
     6541 tgctgacccg catccgcaat gcgcagatgc ggcacaaaac caaggtgacg acaccggcgt
     6601 ccaagctgcg tgagcgcgtc ctcgatgtgc tgacggagga gggctatatc cgcggctatg
     6661 cgcgggtcga ttacgacggt ggcaagtccg agttcgagat cgagctgaaa tatttcgatg
     6721 gcgagccggt catcaaggat atcaagcggg tgtctacgcc cgggcgccgt gtgtactcgt
     6781 cggtgaagga tctgccgacg gtcgccaacg gtctcggtgt ggccatcctg tcgacgccca
     6841 agggtgtcat gtcggattcc cgcgcgcgga acgagaatgt cggtggcgag attctctgta
     6901 acgtattcta gcggagcttt ttgaagcgaa ggtttgatca atgtcgcgca tcggcaaaag
     6961 accagtggca attcccggcg gtgtaaccgc cgcggttgac ggccaagaga tcaaggtgaa
     7021 gggccccaaa ggcgagcttc gccatgtgct cgctgaccag atcatggcca agaccggcga
     7081 tgacggtatc gagatcgcta tgcgcgcgga ctcaaacgag gcgcgtgcaa tgtggggcat
     7141 gtcgcggacg ctggttgcga atttagtgac gggtgtgacc gaaggtttct ccaagacctt
     7201 ggagatcacc ggcgtcggtt accgcgcggc ggtgaaaggc caaaccctgc agttgcagct
     7261 tgggttcagc catgacgtca actatgcgat cccgcaaggc atcgaggtga agtgcccgaa
     7321 gccgaccgag attctagtga gcggtatcga caagcagctg gttggtcagg tcgctgcgga
     7381 gatcagacga tacaggccgc cggagcccta taagggcaag ggcgtgcggt atgcgggcga
     7441 gtacatcctc cgcaaagaag gcaagaagaa gtagcgggat tagacgatga gcgcgttgaa
     7501 caatactttt gcgcggcgga cacgccgggt tcgccattct ctgaagcggg cggccaatgg
     7561 acgtgcgcgt ctgtcggtgt tccgttcctc caagcatatc tatgcacagg tgatcgacga
     7621 cagcgcgggc aagaccgtcg cggcggcgtc gagcctcgac aaggatttca agcttgagaa
     7681 gggcgctgat acgtccgcgg cggaagccgt cggcaagcgt gtggccgagc gtgcggtcaa
     7741 ggccggcgtc aaggacgtcg tcttcgatcg tggcggttat ctttatcacg ggcgcgtcaa
     7801 ggcgctcgca gacgcggcgc gcgaagccgg tctgaacttc tagggtttag gaaagggtct
     7861 acacgtggca cgaggtcccc aatcacgcgg tccgcaaggt agagaccgcg acgaccgcga
     7921 cagcgagttc gtcgacaagc tcgttcacat caatcgcgtg gccaaggtgg tcaagggtgg
     7981 acggcgcttc ggcttcgcgg cgcttgtcgt cgttggcgat cagaagggcc gggtcggttt
     8041 cggccatggc aaggcgcgtg aggtgcctga ggccattcgc aaggcgacag agtcggccaa
     8101 gcgcaatctg gtcaaggtgc cgctgcgcga gggccgcaca ctgcatcacg acgtgcgcgg
     8161 acagcatggc gcgggcaagg tgatcttgcg cgctgcgcct cccggcaccg gcatcatcgc
     8221 cggcggtccg atgcgcgcgg tgttcgagac actcggtatc caggacgtgg tcgccaagtc
     8281 gatcggcagt tccaatccgt acaacatggt gcgcgccacg ttcgatgcgc tgcgccacga
     8341 agatagcccg cgtggcgttg ctgcgcggcg tggcaagaag gtcagcgaga ttgtgtcccg
     8401 ccgccgcgat caggccggtg acggcaccga agcgcaggct taaggcacac gagcaaggtt
     8461 gaagagagat ggccgagaaa cagaaaacaa tcgtcgtgga gcagatcggt agcccgatcc
     8521 gccgccccgg cagtcaacgc gcgaccctga tcgggctcgg cctgaacaag atgcacaagc
     8581 ggcggactct ggtcgacaca ccgcaggtgc gcggcatggt ggccaaggtg agccatctgg
     8641 tgcgcatcgt agaggacgag gccggagcct aaggaacgaa tatcatgcga ctgaatgaga
     8701 tcagcgaccg gccgggcgcg accaagccta agaagcgtct ggggcgtggc ctcggctcag
     8761 ggctcggcaa gacgtcggga cggggccaga agggtcagaa gtcccgctcg ggtgtggcca
     8821 tcaagggctt cgagggtggt cagatgccgc tccaccggcg cctgccgaag cgcggcttca
     8881 agaacatctt cgccaaggat ctgaacgagg tgaatgtcgg tcgggtgcag gcggcgatcg
     8941 acgccaagaa gctcgatgcc aaggccccga tcacggttca ggccttgctg gacgccggcg
     9001 tcatccgccg cgtgcgcgat ggcgtgcggt tactcggcaa tggcgagatc aagaccaagg
     9061 ccaccatcga ggtcacgggg gcgtccagcg gcgcggtcaa ggccgtggaa gcggctggcg
     9121 gaactgtgac gctcacgccg atgccgcgcc gggcgccgaa gcccgacggc gagggcaagc
     9181 ccaaaaaaga tagcaagacg gcggcaaagt ccgccgcacc gaagaagacc gagggggccc
     9241 cggcgaagaa gccggcggca aagaagaagg ctcccgccaa gaaggacacg tccgagtgag
     9301 gtagtgcggc tcttcaaagg gccgcctcat cgtctcgggg aaagaaagag cgccgatggt
     9361 ttccgccgca gaacagctcg tctccaatct gaacttcgcg gctttcgcca aagccgacga
     9421 gctgaagaag cgcatctggt tcacgcttgg cgcgctgatc atctatcggc tcggcaccta
     9481 cattccgatc ccgggcatca acccggaagc gcttgccgaa gtattccggc agcagcagtc
     9541 cggcatcctg ggcatgttca acatgttctc cggcggtgct gtagggcgca tggcgatctt
     9601 cgcgctcaac atcatgccgt atatttcggc gtcgattatc atccagctgc tgaccaccgt
     9661 ctcgccgcat cttgagcagc tcaagaagga aggcgagcag ggacggcgcc agatcaatca
     9721 gtacacgcgc tacggcacgg tgctgctggc cgcgctgcaa ggctacggca ttgccgtcgg
     9781 ccttgagggc actggcaatg tggtgaccga tccgggttgg ttcttccgca tcaccacggt
     9841 catcacgctg gtcggcggca ccgtcttcct gatgtggctc ggcgagcaga tcaccgcgcg
     9901 cggcgtcggc aacggcatct cgctgatcat cttcgccggc attgtcgccg agttgccgtc
     9961 ggccttggtc ggtacgcttg aactcggcaa gcaaggcgcg ctgtcgacgg ccctcatcat
    10021 cgccctgttc gtcatggccg tggctgtgat cgcgtttatc gtcttcatgg agcgggcgca
    10081 gcggcgtctc ttggtgcagt atccgaagcg tcaggtcggc aacaagatgt tccagggcga
    10141 tgcctcgcat ctgccgctga agctgaacac ggcgggcgtg attccaccga tcttcgcctc
    10201 atcgctgcta ttgctgccga ttacggtggc gaatttctcc gcaggccaag ggccggaatg
    10261 gctcaacacg atcacggctc tgctcggccg cgggcagccg ctctatctgc tgatctatat
    10321 ttcgctgatc atcttcttcg ccttcttcta tacggcagtg gtcttcaacc cgaaggacac
    10381 ggccgaaaat ctgaagaagt atggcggctt cctgccgggc attcgccccg gcgagcgcac
    10441 ggcggagtat atcgactacg tgctcacgcg gatcaccgta gtgggcgcgc tgtatttggc
    10501 cgctgtctgc gtgctgcctg agctgctgat ctccaactgg ggtgtcccct tctactttgg
    10561 cggcacgtcg ctgctcatcg tggtgagcgt caccatggat acggtggcgc aggtccaaag
    10621 ccatctgctg gcgcaccagt acgaagggct catcaaaaag gccaagctcc ggggagcgcg
    10681 gcgccgatga tcctggtcct gatcggccct ccgggcgccg gaaaggggac ccaggcgaag
    10741 cgcttggaag accttcgggg cttggtgcaa ctctccacgg gagacatgtt gcgggccgcg
    10801 gtggccgctg ggaccgaact ggggcgtcag gcaggcgatc tcatggaaca tggccagctc
    10861 gtgccggacg accttgtgat cggcctgatc gccgaacggc tcgatgctaa caaggacgcg
    10921 aaaggcttca ttctcgacgg cttcccgcgc accatcggac aggcggaggc cttggaccgg
    10981 ctcctggaga cgcggggcgc ccaagtcgac cggacggtgg tcatcgacgt ggacgacgat
    11041 gcgcttgttg tgcggatcac gggccggttc acctgtgcca cctgcggcga gggctatcac
    11101 gacctcttca agaagcccaa ggaagagggg acttgcgaca aatgcggcgg tacggaattc
    11161 aagcggcggc ccgacgacac cgaggaggtc gtgcggtcgc ggctcaaggc ttatcacgcc
    11221 cagacggagc cgctcgtcgg ctattacggc gggcaggaca aggtgagaac agttaacggc
    11281 atggccgata ttgaggcggt acagaaggag atcgatctgg cgctggatgc ggcgtgagct
    11341 tgaccgagac ggccgggatc ttcggccagt tgaccttgtc aagcggaatc gctacataac
    11401 ggcgtcacat tgtttgatgg actgaccggt cccgggggta gagcctttcc gcggtcggtc
    11461 tctttgtgtt cccgtagggt aaactagctt agatttcagc acgttagcac gtgcgcgagg
    11521 aggcgacgac gtggcccgta ttgcaggcgt aaacatcccg accaataagc gggtcgagat
    11581 cgcgctgact tatatccatg gcattggcga taccttcgcc aaggacattt gcaccaaggt
    11641 gtcgataccc accgagcggc gggtgaacga gctgacggac gccgaggtga ttcagatccg
    11701 tgaggtcatc gaccgcgatt acatggtgga aggcgatctc aggcgcgaag tcgcaatgaa
    11761 tatcaagcgt ttgatggacc ttggttgcta tcgcgggtta cgtcaccgcc gtggtcttcc
    11821 ggtccatggt cagcgcaccc ataccaatgc gcgtacgcgt aagggcccgg ccaggccaat
    11881 cgccggcaag aagaagtaag ttaagtccgc gcgcggccaa gctctctgta tgagcctttt
    11941 gctggcgcac agtcattgat agcgggaata gagcatggct aaggaaaagg ggcgggttcg
    12001 tcgccgcgag aagaagaaca tcacttcggg cgtagcccac gtgcatgcaa gcttcaacaa
    12061 caccatgatc accatcactg acgcccaagg aaacgcgatt tcctggtcgt cggcagggac
    12121 catgggcttc aaggggtcgc gtaagtcgac gccttacgcc gctcaggtgg cggccgagga
    12181 tgccggtaag aaggcatccg agcacggcat gaagaatctt gagatcgaag tgcgcggtcc
    12241 gggatctggc cgggagtcgg ctctgcgggc gctgcaggct gcgggcttca acatcatctc
    12301 gatccgtgat gtgacgccga tcccgcacac tggttgccgg ccgcgtaagc gccgccgcgt
    12361 ctaaacaaag gtaaggccac gaacacgatg ctggacgagg acattcgcaa gcaggagccc
    12421 acggctccgc tcaatgatca agaaagggct accccggtgc tgcaaaagaa ctggcaagat
    12481 ctcatcaagc ccaccaagct caatattgtt cccggacgcg acccgtcgcg ttacgccacg
    12541 atcggcgccg aaccgctcga gcgtggcttc gggctgaccc tgggcaacgc gctgcgccgc
    12601 gtgcttctgt cttcgctgca gggcgccgcc atcacctcgg tgcatatcga cggcgtgctg
    12661 catgagtttt cttcgattcc gggcgtgcgc gaggatgtga cggacatcgt cctgaacatc
    12721 aaggaaatcg cgctcagcca gcattcggag ggcgtcaagc gcatggtgct tcagaaagaa
    12781 ggaccaggca tcgccacggc gggcgacatt caaaccggct ccgaggtcga aattctgaat
    12841 ccggagcacg tgatctgcac gctggacgaa ggcacggcga tccacatgga gttcacctcc
    12901 tcgtcgggta agggctatgt gccggcggag cgcaatcgtc ccgacgatgc gccgatcggc
    12961 tttatcccgg tcgactcttt gttcagcccc gtgcgcaagg tttcctatcg cgtggagaac
    13021 acccgcgagg gccagatcct cgactacgac aagctcacca tggagatcga gaccgacggc
    13081 tcagtgcggc cggaggactc cctcgcgctt gccgcgcgtg tcgtccagga tcagctgtcg
    13141 gtgttcgtca acttcgaaga gccgaagaag gagcagacct tcgagcgcca gccggagctg
    13201 gtgttcaacg ccgcgctcct caagaaggtc gacgagttgg aattgtccgt tcgctcggcc
    13261 aactgcctga agaacgacaa catcgtttat atcggcgacc tcatccagaa gaccgaggcc
    13321 gagatgctgc gcacgccgaa tttcggccgc aagtcgctca acgagatcaa ggaagtgctc
    13381 gcttccatgg ggctgcattt gggcatggac gtgcccgatt ggccgccgga gaacatcgaa
    13441 gagctggcga agcgcttcga agaccactac taagcaattc ggccgcggtc acgcggccct
    13501 acaagctccg taagggagga gttcatgcgt catcgcaaag caggccggaa gctcaaccgg
    13561 accgccacgc accgtagcgc gcttctgtcg agcctggcat gcgcgctcat caagcacgag
    13621 cagatcgcga cgaccctgcc caaggcgaag gaacttcgcc gggttgccga ccgtctgatc
    13681 acgcttgcca agcgcggcga tctgcacgcg cgccgtctgg cctttgcccg tatccgggac
    13741 gacgccatgg tgtcgaagct gttcgagact ttggggccgc gctacgcgga ccgtccgggc
    13801 ggttacacgc gcgtgctgaa ggcgggcttc cgttatggcg actccgcgcc gatggcggtc
    13861 atcgaactgg tggaccgcga cgaggacgcc aaggggctcg actccggacc gacccaggag
    13921 gtcgccgaag agggcgaggc cgcggccgcg taagcgagac ggcacattcc agaatccaaa
    13981 aggcgcgcca cgtcagcggc gcgccttttt cgttgtcatg ctcggaagtt cttagaactt
    14041 ggagcggtcc cggccggtgg tgacaggcct gccggcggcc gcctcgcgct tgtcgtaggc
    14101 ctcgatggcc ttcgtcttga tggcgctcac gccggtgttg aagatgccga cgataatctt
    14161 cttggcctcg tcatcctcga cgccgtccgc atcgaactcg gcctcccact cgatctcgac
    14221 gcgatcgggc tcgtcgtccg cctcgacctc cagcgtcgcg gagtagttct tcaccggcag
    14281 cgggccttcg atgatctcgt aactgtagct cgtgtcgtcg gtctcggtca gcttctcctt
    14341 gatcttgccg ccgtcgccca gcgtgaggat gcggtaggtg tcgctgcctt cctcgatctg
    14401 ctcgcaatcg gccactgcct catgccaatc cttgatggcg cagaaatcgc cgaccacggc
    14461 ccagacctcg gccggcgtgc cgggggcctc tctgcgcttc ttgacctcga cggcctcggc
    14521 tgcggcgctc caggcgaagg tggcggccag agccaccagg gtcagcttga aaactcgcat
    14581 gcgtctctct ccactttttc gctgttgtga acggcaaaag ctgtcgcccg cgcggcagtc
    14641 tagggttcaa acgcgggctg cggcaacctg gtttccctgg attctcgccg caagaaggcc
    14701 cgttttcttt ggaagatcaa cgcgtcgtgc tggtatatcc agacagtgtt ttggccgtcc
    14761 tacagatccc taacaggaca cgagatgacg cttttggaaa aactttccgg gaagattggc
    14821 attctctgct tggcaggcgc gtccctcctt ttgagcgtgc cgggcacgct cgcccaggag
    14881 cgggcggtgc cgaagaacca ggcggcgatg aagcagtcct tctcgccggt ggtgaagcag
    14941 gcggcgcccg ccgtcgtgaa cgtctatgta cgccaccggg tgaagcagtc ggtttcgcct
    15001 tttttcaggg acccattctt ccgccgcttc tttggcgaca agttcggcgt gccgcgcgag
    15061 cgcatccaga actcgctcgg ctcgggcgtg ctggtccgcg gcgagggcgt ggtggtcacc
    15121 aactatcatg tcatccgcgg cggcgagaag tccgaggtca cggtggccct gagcgacggg
    15181 cgtgagttcc cggccgaaat catcctgaag gacgagaaga ccgatcttgc cgtgctgcgc
    15241 ctggacgggg agggcgccaa gtttccctcc atcaagttcg ccaactccga cacgctggaa
    15301 gtcggcgacc ttgttctggc catcggcgat ccgttcggcg tcggccaaac cgtgacaagc
    15361 ggtatcgtct cggggctggc gcgcaccaag gtcggttttt ccgactacca gttcttcatc
    15421 cagaccgatg ccgccatcaa tcccggcaat tcgggcggcg ccttggtcga tatggacggg
    15481 cgcctcgttg gcatcaacac ggcaatcttc tccaagagcg gcggttcgca cggaatcgga
    15541 ttcgcgattc cttccaacat ggtgcgcctg gtggttcagt ccgcgctcaa aggcggcaag
    15601 cttcagcggc cgtggtttgg cgcatccttg cagccgctca acgccgacct cgccgagtcg
    15661 atagggctcg accggccttc aggcgcgatg gtgaccagcg tgcacaagaa tggacccgca
    15721 cggggcgccg gcttgcaagt aggcgacgtg gtcgtgagca tagacgacaa gaccgtgaag
    15781 gacccggaag ctctgcaata tcgcttcgtc accaaggggg tcggcgggaa ggccaagctt
    15841 ggcgtctacc gcaaccgcaa gctaatgaac gcgacgatct ctctgatcgc gccaatcgaa
    15901 gacccgccgc gcgacatacg cgagctggag ggccggcatc catttgccgg cagtaaggtc
    15961 gcgaacctgt cgcccgcagt ggcccatgag atcggcatgg atgacgatgc taagcagggc
    16021 gtcgtggtgc tcgacgtgac ccgaggttcg aatgcgggcc ggctgggctt gcgccgcggc
    16081 gatatcgtcg tcgggatcaa caaagagaag gtttcgtcgg tcgaacagct gagcctcttg
    16141 cttcagttgg acaccggcgg ctggcggcta tccgtcgagc gtggcgggaa ggtgttcaat
    16201 ctcgccattc agggctgacc atgggagagg atgcggccac gttgtttgag gctgcggggc
    16261 ttggcgatgc tgcgccgcgg ccgcttgccg accgtttgcg gcccaagtcg ctcgacgagg
    16321 tcatcggcca ggaccatctg ctcggagacg gcatgttggg ccgcatggtc cgggttggcc
    16381 gcctcagctc cgtgatcttc tggggcccgc cgggcaccgg caaaaccacg gtggcgcggc
    16441 ttctggcgga ggtgacggac ctccacttcg agcagctctc ggcgatccag tccggcgttg
    16501 cggatttgcg caaagtgttc gacacggccc gggcccggcg gcaagaggga cgcggcacgc
    16561 tgctctttat cgatgagatc catcgcttca accgctccca gcaggatgcc ttcctgcccc
    16621 atatggagga cggcaccatc gtgctcgtgg gcgcgacgac ggagaacccg tccttcgagc
    16681 tcaacgcggc cctgctgtcg cgggcgagcg tcctcacctt caagcgcctc gatgacgatg
    16741 cgctcgaagc gctgctggcg cgagccgaga cccacgaggg gcgcaaactg cctacaacgg
    16801 acgatgcgcg caccgcgctg atctctatgg cagacggcga tgggcgcggg ctcatcaacc
    16861 tggcggaaga cgtgttcctc gcggttggca aggacgcgaa gctggtgctc gatcgcgaag
    16921 ctctcgtgga gttggtacaa cgccgggcgc cgatctacga caagggccag gacggccact
    16981 acaacctcat cagcgctttg cataagtcgg tgcggggctc cgaccccgac gctgcgctct
    17041 actggctggc gcgcatgtta gtggcgggcg aggacccgct ctatatcgcg cggcgcttgg
    17101 tgcgcatggc ggtggaggat atcggcatgg ccgacccggc cgggctcgtg caggccaatg
    17161 ccgcgaagga cgcttatgac ttcttaggct cgccagaagg tgaactggcg ctagcgcaag
    17221 cgaccgtcta tctcgcatgc gcgccgaaat ccaacgcggc ctatgtggct tacaaggcgg
    17281 cgatgggtgt ggccaaggag agcggctcgc tgttgccgcc gaagcatatt ctcaacgccc
    17341 cgacgaggct gatgaagcaa gaaggctacg gccgcgatta cgcctacgat cacaacgaga
    17401 cggaagcctt ctccggccaa gactatttcc cggaggagat ggagcggccg gacttctatg
    17461 cgcccacggg gcgcggcttc gagcgtgaga ttagagagcg gctcgagcgc tggggggaga
    17521 tccgggcgaa gcgagcgcag cggtgacgag ggggaggggc tgatatgcaa actctattat
    17581 gtgtagcggc tggcggcgcg cttggcgcct ctgggcgcta tctcgtcggc atcgcttcgg
    17641 ggcgcctgtt cggcctcgat tttccctggg gcacgcttat cgtcaacgtg ctgggctgcc
    17701 tcgtcatggg cctgctgatc gaggccatgg ctctgcgctg gacggtgcat aacgatctgc
    17761 gcgccttcct cgccgtgggg ctcttgggcg ggttcacgac attctcggcg ttctcggccg
    17821 atttcgggct ccttttcgag cgggacgcat atctcagcgc ggggctctat ctctctgctt
    17881 ccgttggact ttccatcgcc gccttctttg ccgggctggc tatcgtgcgc gccctctaaa
    17941 gatgccgccg gtccaaaccc gccatgtcac ggaagacgag gacggcatgc ggctcgaccg
    18001 ctggttcaag gcccattacg cgagcctgcc ccatagccgg ctggagaagc tgctccgcac
    18061 gggccaggtg cgggtcgacg ggggacgggt caaggcaagc acaaggctta gcgcgggtca
    18121 gagcgtgcgc gtaccgccgc ttcccgacgc gcccccgccg ccgggtgaga aacgcacgct
    18181 gtcgaaagcg gaccgggcgt ttctgaaaga gatcacgctc tacgaggacg acgatctcct
    18241 cattctcaac aagccctcgg gcattgccgt gcagggcggc accaagacga cgcggcacat
    18301 cgaccggctg ttggagggct taagcgacag gcccgagacg cggccccggc tggtgcatcg
    18361 cctcgatcgg gacacgtccg gcgtgctggt cgtcgccaaa cggcgtagcg tggcggcgaa
    18421 gctcggccgc gccttccaga cccgttcggt gcggaagatc tactgggcgc tggtgcacgg
    18481 cgtgccgaag ccgccgcaag gcaagatcga cgcggccctg gtcaaggcag caacgcccga
    18541 tggcgagcgg gtgcgcaaag cgcgcgccgg cgagcaggcg caagcgcagt ccgccgtgac
    18601 ccattacgcc gtcatcgacc gtgccggtca gcaggttgcg ctcatgtcgc taaagcccgt
    18661 caccgggcgc cagcatcagc tgcgcgcgca tatggcgatg gccggacacc ccatgctggg
    18721 cgacgagaaa tatccgagcg acatcgatct ccccgacggc atcgagcgca agcttcattt
    18781 gcatgcgcgg cgcatcgcct ttccgcatcc gagcggggag ggtgtggtcg acgtcacggc
    18841 gccgctaccg ccgcatatgg aacaggcgtt ctccgctttc ggcttcaccg ttaaaggcgg
    18901 agaggttgag gaggcggcgt tcgaccgcga tgcgtgtacg cggtgaggag cgggcgaagc
    18961 gcggcatcat agagtatcgt catcttcgcg cggcactcta cgcgccagcc caaacgtcct
    19021 atgaaagagg atcgaccatg cccccgaaga caagctggtt tactcgtttt gcccaactcg
    19081 ccgcgcacat cactggacgc ccaaggtgct tcttcctggc cgtctccgtc atcgtgctgt
    19141 gggcgctcgc cggaccgttg ttcgattaca gcgacacctg gcagctggtg atcaacacca
    19201 gcacgaccat cgtcaccttc ctcatggtgt tcgtcattca aaacacgcag aaccgcgaca
    19261 cggcggcgat gcacatcaaa atcgacgaac tgatccgcgt tacggagaag gcgcgcaatg
    19321 tccttctcga cctggaggag cttgacgaga acaagctgga ggccatgcgc cgcgactatg
    19381 aacgcttggc cgaagagggc cgcgcgcgcg ccgaagcagt cgagttgacc tgacgatggc
    19441 cgagaaggac acgaacgcgc cgcagaacgg cgcctcacag aaatcgagtc ttcccaagcg
    19501 gttctacaag gacgtgaccg tggcggatga ggatggccac gccgccatcc tgctcgacgg
    19561 gaagccggtg cgcacccccg gcaaggcgca gcttgcgttg ccgagcaggg cgctcgccga
    19621 ggccatcgcc gaggagtggc gggcgcaagg gaagcatatc gatccggcca ctatgccgct
    19681 gaccaagctc gccaacagcg ccatcgacgg cgtggtgggg caggccgacg cggtgatcga
    19741 cgacatcctg gcgcatgccc gtagcgatca tctgtgctac cgggcgagcg gaccggagaa
    19801 gctgctggcc gcgcaaggtc agcattggga cccggtgatc gcctgggcga aggaggtgct
    19861 cggcgcgccg ttcaacctta ccgaaggcgt ggtccatgtg gcccagccgg acgcttccat
    19921 cgaggcgctc aagggcgagg tcgagaagct cgacgccttc gggttggcgg cggttcacgt
    19981 catgaccacg ctcacaggct cggccctgct ggccctcgcg gtcgccttga agcggctgac
    20041 cccggaggag gcctgggcgg ccgcccatgt ggacgaaaac ttccagatca gccaatgggg
    20101 cgaggacgaa gaggccaagg cccgccggga ggcccgcaag cgcgacttcg acgccgcggc
    20161 gagggccctc gaactttccg aatcctgaga atcggttagg gccgggcgct gacaggccgg
    20221 tcgaagcgcg cccttcgctc cagccatttg cctcctttcc gccccatgct attcacctgg
    20281 gcaaacgcga caccgccaag ggagccccca tgaaagaagt tcttgaagag cttgaagatc
    20341 gccgtgccaa ggcccgcatg ggcgggggca agaggcgcat cgacgcgcag cacaagcgcg
    20401 gcaagctcac cgcgcgcgag cggcttcagc tcctcctcga cgacggttcc ttcgaggagt
    20461 tcgacatgta tgtcgagcat cgctgcgccg atttcggcat ggccgagacc aaggtgccgg
    20521 gcgacggcgt ggtgacaggc tggggcacga tcaacggccg ggtggtctat gtaatcgcca
    20581 aggacttcac cgtgttcggc gggtccctgt cggaagccca cgccaacaag atgatcaaga
    20641 tccaggacat ggcgctgcgc aaccgcgcgc ccgtcatcgg cctgttcgac gcgggcggtg
    20701 cgcgcatcca ggaaggcgtg gcggcgctcg gcggctatgg cgaggtgttc ctgcgcaacg
    20761 tccaggcgtc gggcgtcatt ccgcagatca gcgtcatcat gggcccgtgc gctggcggcg
    20821 acgtctattc gccggccatg accgacttca tcttcatggt gaaggacacc tcctacatgt
    20881 tcgtcaccgg tcccgacgtg gtgaagacgg tcaccaaaga ggaagtgacg gccgaggagc
    20941 tgggcggggc ggtggtgcac accaccaagt cgtccatcgc cgacggcgct tacgagaacg
    21001 acgtcgaggc gctactgcag atgcgccgcc tcatggacta tctgccgtcg tccaatcttt
    21061 ccggtgttcc tgagttgccg acgcgcgatc cgtggaaccg gccggagccg tctctcgaca
    21121 cgctgatccc cgataacccg aacaagccct acgacatcaa ggagctgatc cataaggtcg
    21181 tggacgaggg cgacttcttt gagatccagg agacctacgc ggggaacatc gtcaccggct
    21241 tcgcccgcat ggaagggcgg acggtcggca tcgtcgccaa ccagcccatg gtgctcgccg
    21301 gcgtgctcga tagcgacgcc tcgcgcaagg ccgcgcgctt cgtgcgcttc tgcgattgct
    21361 tctcgattcc cattgtcacc ttcgtggacg tgccgggctt cctgccgggc accgaccaag
    21421 agtatggcgg gttgatcaag cacggcgcga agctgctctt cgccttcacc gaggcgaccg
    21481 tgccgaaggt caccgtcatc acccgcaagg cctatggcgg cgcttacgac gtcatgagct
    21541 caaagcatat tcggggcgac gtgaactatg cctggccctc ggccgagatc gccgtcatgg
    21601 gcgccaaggg tgccgtcgag attttgtatc gcgccgacct tggcgatccg gagaaggtcc
    21661 aaaagcgcat cgacgactat caggagcgct tcgccaatcc gttcgtggcg gcggagcggg
    21721 gctatatcga cgaggtgatc gcgccgcgcg gcacacgcgc acgcatctgc cgtgcgctgc
    21781 aaatgctacg caacaagcac gtcgaaaatc cctggaagaa gcatgataac attccgttgt
    21841 aagggcttgg tggaggacga aaaatcgctg agcgagcttg aggagttaat ttagcgcaag
    21901 atagggcttg tatctgccat ttttgcccca tatctatgca gcatcgacat ttggccggac
    21961 aatatcgccg ttttgccttc gccttcgctg attgccaggc gcgcgttcag accctccaaa
    22021 catcgacgaa taggcttcgg actgcgctat agatgcggtc tcaagtgcaa ctgactacag
    22081 cgaaaggacg accccttatg atcaagggca ctgtaaaatt ctacaacgag caaaagggct
    22141 tcggcttcat cgagccggaa gatggcggca aggacgtgtt tgttcatgcc accgcgcttg
    22201 agcgcgccgg catgggcagc ctcgccgagg gccagaaggt ctcttttgac accgaggtag
    22261 acagccgcag cggcaagacc gcagtggcaa gaattgaggc cgcgtagtcc taacggaccg
    22321 cacgatcttc aatgaatatg agccgtcccc ctcggggggc ggcttgttga ttcttggaca
    22381 gatgtggtct tgaggaccgc tgttacggtc cgatgcgcgc gcagagcgga ccgttgagcg
    22441 ttctctgggc cgaggccgtg tagttgacgg ccagcccgta attcttgagc gcgccgtcca
    22501 tggcgctgcg cttggcatcg gcggcggcct tgctgccttt cttcggcttg tcgcccttcc
    22561 ccttcttctt gctgacgccc agcgtgtcgg agatggtcca ggccatcttg ccgaggccaa
    22621 gccaggtatc gcgcaccgag ccgccttcac tccggatcat tccagccttc gccagcgcca
    22681 cggcacaggt cgctgccgcg ccgaccggaa acggccggcg gcagaaggga agggcagggt
    22741 cacgacttcc tcggcagact tgccgtcctc cgaaagtctg attacgtcga tgcgctgtcc
    22801 gaccggtgtt ccaccgaatc cgatgcccat gatgccgacg accaaacgtc cgtcccaaga
    22861 gccccactga tcgccggtca gaaacgcggc agaggatgtg ccttgagact tttggcattc
    22921 gaaggacgtt tcgggataca tatcccacgc agtaggacat agttcccgca cgaaacgtgc
    22981 aacgatggtt ccgctagtgc ggcggtcgtg tcgtaaggag cagaatcatg acgaacagag
    23041 tgatgacgct tgccggtgtc gcgattgccg ggctgctggc tggcccggcc tttgccgaag
    23101 ggccggtgga gacgacggtc acttacgagt gcgccggggg caaatccatc gtggcgatgt
    23161 acacgtctga cgaggtggcg ctcaccttcc acgacgggcg caagctggcg cttccgcaag
    23221 tcatctcggg gtccgggacc cggtacgcca acgccgacga gagcatccag ttctggtcca
    23281 agggcaagac ggccttcgtc acggaaggct cggatgacaa cgtgacctac aaggattgcg
    23341 tcgccgactg agccgggccg gcccgaaggc gggacccgcc aagattctct tccccagtgt
    23401 tgctgcgctg caattgcgcg ttaaccgctc ctcgtataat tagcggcctt caatctatgc
    23461 tgaccttcga gggggtaacg atgcgtcttg gcgcgtgggg gtggagtctg gccgtttgtg
    23521 ccgtgttgct gtcggggagc gcgttcgccg tcagcctgtc gtcctcgctc aagaactatt
    23581 acgctgagtt cgagatcgtc ctcgagtgcg aggtaaagga gcatctctcg acggccgagg
    23641 ccgacgccgc cgaggcggcg atcgacaaga tcgaagacta tttcctggcg aaagactcct
    23701 cgatcaacac cgagagcctc cagaagaagg cggttgccga caagaacgag ggcctacgga
    23761 ttatggcgcg gagcggtggg ggccttacgc cctattgccg gcagtccctg cgggagctct
    23821 tgaccaaggc gaaagagatc ggaccggccg cccagtagca tatcctccac attttgcccg
    23881 cgcgcctatg ggccgtcctt gatgcggcaa ggcctcgatc ctagatgtcg gataggccgt
    23941 gccggggcaa cattggagtg acgagaatgc gtccaagagg gctttccgct tttgtgtttg
    24001 ttgccgcgct gctggccatg gtagtgccgt cggttgccca atattcgaaa gccataaaag
    24061 gcgcctgccg gaacgactac aagcggttct gttcgaccta tgccgtcgac gatcctgcct
    24121 tgcgcaaatg catggacaaa gccggaccca gcctgtcgca gagatgcgtc gccacgctcg
    24181 tgaattcggg cctggtgagc taccagcgcg ccacccagcg ctgggggcac aagctcgaga
    24241 aggtccaata gtcgtcggaa cgaggatcgc gcggcgctgc ccgccggtct gttgccaaat
    24301 gcataatcaa gagcggcgcc tttctcaggt aatgcaaagt tttgctttgc gtcttattca
    24361 ctctcagtgg ccaagactta ttcacaggca gtcttgagat tattaccaat gttcctatag
    24421 acattgccag ctgggcccgt tgctttgctc taagttccag ctgcatcgac tcgatgaaga
    24481 gactaaggag ccccccaaaa tgcacaaatt tgcactgact tttgcaatcg ctatcagcac
    24541 gtttttcttg gccggttcgc cggcgtccgc cgtcgattct gccacggcgc ttcgggtcct
    24601 cgatccggac aatgacggca ccatcgacat gagagaggct ctcaggggcg ccaagagggt
    24661 gttcaaggcc atcaatcccg accgggacgg tacgctcgac gctgccgagc tcaggggacg
    24721 cctcagcgcg gctggcctca gggcggccga tccggacgac gacggcacgt tgagctggag
    24781 agagtacaag aggctcgtca aagcgctgtt cgtgctcgcc aatcccgacg atgacggcac
    24841 gatcgacctg agggagcttc gttctccggc cggtcggcgg ctgctgaagc tcatctatct
    24901 ctaggccttc acggtaaggg cgcgggccgg atttccggtc tcgcgctctc atagcgtctg
    24961 gggccgggcc ctgccattag caagacacaa accatcttca tgagagggtt atacggacgg
    25021 ctgacctcaa gacggggagg gcggctgaac ggtatcttgc ggcgggggag aagccggtga
    25081 cggatctcta caagactttg ggcgtgaagc ggaccgcggc cccaaaggac atccacaaag
    25141 cctatcgcaa gaaggccaag tcggcgcatc cggacagcgg aggctccgtc gaagccttca
    25201 acacgctggt tctggcctat tcggtgctgt cggataaggg tcgccggacc cgctacgacg
    25261 agaccggcga aatcgacccg ccgcggcccg acaatttcga tggcggcgca atcgagatca
    25321 tcgccgagaa gctgggcctg gtgatccacg ccgagcagga tctggccacg atggatgtgg
    25381 gccggctgat cgaacaggcg attcgcgacg acattgccgc ccgcaaggcg agcgtcaccc
    25441 atcagcagcg cgctggggaa cggttggcgg ccttacgttc gcgcaccaag cgcaaggccg
    25501 aaggggacgc caatctggtg gcccgggtgc tcgattggca cgaactggcg atccggaacc
    25561 acatcaagaa gcacgaggag tcgatccgct tcttggagcg ggccctcgaa attctgcacg
    25621 gctattcctt tacggacgac ctcgcggccg cgcaggccga ggaggtggcc ggcgctctca
    25681 atgacattct cgtggccctc gacgaactcg ccgcggtcct caacacgacc ccgtcccggg
    25741 ctgaggccat gccgcaaggg gccgggccga ccgccttcgg ctagtgcttc aaggggtggc
    25801 cgaaaggcta tgtctcgagt agatttttcg gcgatctctt ggggggtggg agtttgcgtc
    25861 gatatttcct catcgccatg acgtgctgca tggccctggc tgcttgcctg ggggccttgg
    25921 cggccgcgcg cgcggagacg cagaccgtgc cgcctttgcg gaagcttccc tgggagtcgg
    25981 acaccgccgc gacgcagccg ccagaacctg cagtctcccc ctccgtgtct tcgcccacgc
    26041 cggcgcctga ggcggcctcc gagacggact cagaggaaga gactgacacg gcgaaagacg
    26101 agccggacga aggcgaaccg gaggaagtag ctgaggctgc cgacctgccg ccagtgcccg
    26161 cgcatccgac cgtgacgatt atccgggaaa agctgactga tccgaaggtc acgaaggacg
    26221 cggccgatga ggacgtcgct gggcttcagg ccttctatcg tgcttggggc gagccaccct
    26281 tgtgggtcac ggagacggga ctgtcgacca aagggaaggc ggccatacgc gagatcggca
    26341 aggccgacga ctggggcctc aaagcggacg acttcaccgt gccggaggcc gatgtgcagc
    26401 tggcgacagc agaggcccag gcccttgccc agatcacgct cgatctcgcc attctgaaat
    26461 atgcgcggct ggcgagcggc ggcgagcacg tgccgtcgga gattagcgac ttgttcgatc
    26521 agtcgccgcc cttccgcgac cccaagattg tcatggccga tatcgccgcg tcggattccc
    26581 cggacatcta tctgcaaacg ctgcacccgg cccacgagca attcgaccgt ctgcgcgatg
    26641 cgttgatcga ggcccggaag gccgatgagc cgaaggaaac cgacatcagg cggctcctca
    26701 tgaacatgga gcgctggcgt tggatgcccg aggagctcgg gccgctctat gtgtggctca
    26761 acacgcctga gtacatgctc tacgtggtga aggatggaga gacggtcttc aaggacaaga
    26821 cactggtcgg caccatcggc aatcccacac cggtgttttc cgccgacatg gagacgattg
    26881 tcttcaatcc cgattggatc gcgcctccga cggtcttaaa ggagaaattt tggccggcat
    26941 tgaagcgcaa gtactacgac attctcaagt ctaacaagat cagcgtgagc tatcgaggca
    27001 agcggatcga tccgaagaag atcgattggc agcggaccaa cattcacaag ttcaccttca
    27061 ggcagaaagc gggcccgaag aacgtgctcg gcaaggccaa gttcctctat ccgaacaagc
    27121 acgtggtcta tatgcacgac acgcttccgc cacggcgcaa gatcttcaaa gaggataagc
    27181 gcgacattgg ttatggctgc gtgcgcatgt ccaagccgca ggccttcgca gcagtgatgt
    27241 tggccgagga tcaggaactg ccacagagcg acgtcaagaa gttctggaac aagagcgtca
    27301 acaaagcggt gagcatggag aagaagcctc cggtccacac gacctatttc acggcggtgg
    27361 tggatgagga ggggaaggtc tcgaccttca aggacctcta cggtcttgat cgcatgcaag
    27421 ccaaggccgt ctttggcagt gtggatggtt ttccgaaacc gccgcccgag aaaaagcgcc
    27481 tcagccagag cgttgccgcg tcctcgcgcg acagcaacac gggtgcaggt tccaccaact
    27541 ctctcggttt cggggactga gttccgcgct cagctcgcgg cctgcggccg gcccttgcgg
    27601 ctgacgcgtt gagccttccg gcctgcgcgc ccgtgccacc acaccgcgac attgtctccc
    27661 aacatcaaga ggcagggcgt taacacgagt gtcagcatcg tcgcgaatgc caacccgccg
    27721 gcaatagaac ttgagagctg cgtccaccat tgcgtcgacg gcgctccgac ggagacgtct
    27781 tggccaataa tgtcgatgct aagactgaag accatcggca tgaggccgag aaccgtcgta
    27841 atggacgtca gcaggacagg gcgcatacgc tgcgcagcgg tacgcaggat ggcttccttg
    27901 gccggttggc cccgtgacct caagtcattg taggtgtcga tcagaacgat gttgttgttc
    27961 acgacaatgc cggcgagcgc gatgattcca atgccgcaca tgacgatgcc gaacggttga
    28021 tcggtcgcca acaggccaat catgacgccg gccgtggaga agacgatcgc gctcaacacg
    28081 agggcggttt ggtagaagct gttgaactgc gtgaccagaa tcgcgaccat cataaagatg
    28141 gcgatgaaga aggcctttat caaaaagttt cccgcctcgc gctggtccgc gtcctgaccc
    28201 ttgaagcgca cggtcaccgt aggatccaag tcggtctcgg cgaccgcagt cttgagttta
    28261 cgcacctggt cgtcgaccag cagtccctcg gccacatcgg cttgaatttc gagcacgcgg
    28321 cggccgtcgg tgcggttcaa attaccaatc ttctgggccg gttcgaagct cacgaagttg
    28381 ccgataggaa cttggccttt atcggttggc acgcgcagcc gattgagctg ttcgagattg
    28441 cgctcggcga gggggaggcg caggcggata tcgacttctt cgtcggcatc gtccggccgg
    28501 tattcggcaa cgaagatccc attggtgagc atgctcacgg cttcgccaag cacggcgaca
    28561 tcggccccgt tgcgcgcggc cttttcccgg tcgactttga ggcgccactc gataccgggc
    28621 aacggccggc tgtcttgctc atcgacgaac ccgccgatct tattcatttc tgcgcgtagc
    28681 ttcgtgacga catccggcat cttctctgta tcgcgagacg tcacatgtag ttccaccggc
    28741 ttgccagccg taggtccgcc tttctgtttg cgtacctcga ggataacgcc tggaatgtca
    28801 gccgtacggg tacgtacctc tttgagtata tccgaggctc tcgggcgggt acgccaattg
    28861 gtcaattcaa actggacaac accgatgacg tcctcggccg atccggtttg cgacggcggc
    28921 gccccggaac cgatcgtctt tgcatagaca gtcttgaagt gcggcatgcc gaggagccgc
    28981 gtctcgacct tgcgcacgac ggcatccatt tccgtgactg ataaatcgcc gcgcgcacga
    29041 acctggacct gggcaatgtc ttggtcgacg tcaggaaaga attcaacgcc gcggccaaac
    29101 atgccgaatg cggtgtaagt gccgatcaga agagccacgg cgacgcacag aaccttggcg
    29161 gggtgggcaa gaagccgctt gagtgtgttg agataagcgc cggccgcgcc gcctatcttg
    29221 ttcaggtcgc catcttcggc agctcggatg gtctgcagca gactgccgtc ccccttgtct
    29281 gttttgccga aaataccgcc gagcacgggg ataaacacaa gcgccatcgt caacgaggcc
    29341 gccagcgtta cgagaaccgt gatcggcaaa aacttcatga actccccgac gatgccgggc
    29401 cagaacagca atggtaggaa caccgccaga gttgtcgcgg tcgaggcggc gatcggccat
    29461 gccatacgct tggccgcttc cgaataggcc tgtttgcggt ctttgccttc ctgcattttt
    29521 cggtcggcga gttccgtggt gacgatggca ccatcgacga gcatgccgac aacgaggata
    29581 aggctgaaca agacgacgat gttaagcgtg aggcccatca aggagatggc aaggattccg
    29641 gcaagaaacg agcctggaat tgccaacccg accaagatgg ccgaccgcac gcctatggcg
    29701 gcgatgatga cgatcatcac cagcgcgatc gcggcgagca cattgttctg aagatcgctc
    29761 aggatgtcgc gaatttgctt ggacttgtcc tgcatgaagc tgacttggac gcccggcggc
    29821 caagaggctt gttcctcctt aacgacagcg cgcacctctt cgattgtctc aatgatgttt
    29881 gcgccaacgc gctttttgac ttccagcgcc aaggccggat tgccgccgac gcgcgcaaac
    29941 ccttgcgggt ctttgaaggt gcggcgaatc gaagcgacgt cgctgaactt gaccaccgtg
    30001 gtgccgacaa ccttgatcgg gagattcagt atgtcctgaa cttcttcgat gacacctgga
    30061 accttaagca ccatgcggcc ggcggcagac tccaatgcac ccgctgcgac cagccggttg
    30121 ttgcgcctga ctgtggtcaa cacgtcggcg aaggaaacgt tgtaggtttc aagcaccatc
    30181 ggatcgacga cgatctccaa caactcttcc cggtcaccgc cgatatcgac gtcgagtacg
    30241 cccccaaggg cttcgatttt gtcccgcaga tcgcgcgcaa tgcgcaccaa ggtgcgttcg
    30301 ggtacattgc cagacaagcc gaccgtgatg acgggaaaga gggcgacgtt gacctcgttg
    30361 acccggggtt cgtcagtctc aggaggtagc tcggcgcgag caatatcgac tttctcgcgc
    30421 acgtcctcga gagctgtatc gggattaaag cctgcatcga attcgagcgt gacggatccg
    30481 tagccttcct tggccacgga gcgcatctct ttgatgccct cgatggactt gagttccttc
    30541 tccatggggc ggatcaggag tcgctcggaa tcttcgggcg aaatgccttc atacgtcatc
    30601 gagacgtaga tgatcggaat ctgaatgtcc ggttcggctt ccttggggat ggtcgtatag
    30661 gcaaccgcac ccatgacgat aatcatgaag aacgcgagcg cgaccgtacg cgttcgcgca
    30721 aaagcggcgt cgatgatgcc gttcattgag cggccgtcgg ctcgtcgaca cggcgcgttg
    30781 tcaaacgtgc cgctgcctct ttcgaaccgt ccatgccggt gctgccctgt tcctcggcga
    30841 cttggacctg ctcgccgatc tgcacgtagc cgtgacccag cgtaatgatc cgcgcttgag
    30901 gcggcaggcc gatcacccaa gcaccgctgg cgtccgacat gacgatggac gcctcatgga
    30961 agacgacgcg gctgtctttg ccgaccgtct tgacgccgag cttgcctgcc tcgttgaggg
    31021 acagatcggc cggagaaacg aattgcgcca tcaccgatcc cgtggggatc cttgcctcgg
    31081 cgctgatgcc ggacggaatt gcgccgtccg gattgggaag ttcgatctcg acccggaagg
    31141 tccgcgtccc ttcgtccgcg cgcgcggcga tgtagcggat ctttccttca cccttctgac
    31201 cggtcgcaaa ggtgacagtt gccttctgac cgaccttcag atcggtaatg tcctgctggg
    31261 tgatctgaac actgacgacg agggggtgat tgtcgacaat tgtcgccacc tcaccattca
    31321 cgtcgacata ggcgccgagc tcgataggaa ccgttagcac ttctccctcg aacggcgctt
    31381 tgatgtctag gcgctcctgc tcgatcatcg ccacttcaag ctcgtgcttc gcggtctgta
    31441 acgacgagta gatttggtcc gattgtcttt gtgtctggta gcccttcttg ccaagagcct
    31501 gcgctgcttc gtatgcgctt tcctgctcgc gcagtcgcgc ctttgcgcgt tcgatccgcg
    31561 cctcgcgggc gttcggctcc agcctggcga tgacatcgcc ggccgcaaca gtgctgcctt
    31621 cgtcggccaa gatctcggcg atctgtccca aggtctcggc gcgcacggtc acggtccggt
    31681 tcggctcggc ttgcccttgt gcgacgacga tttgcgatac gggtttggct tcttgcgtgc
    31741 gaaccgcaac tcgcatggtc ggagcggcat cggccactgg cgtggtgccg tctgcctctt
    31801 taccaacggt tctcaggaaa tagccgccaa gcacccacgc cgtgagggcg agcgtcaaca
    31861 ccgcagcgat gatgtatgat tttttcaaag cctaacgctc caactcgttc tcgctgcttt
    31921 gcacctgctt ggggtcacgc ggatggccgc gcggaggccc gtttgtggcg tgccgaacct
    31981 tgccgttcgg cgtcccattc atcgatgaca ttcaggaagc tctcaatgag cgcctcataa
    32041 aaggcatcca actcctcgag ccgctcatgg gtctccgaca tgtcctccgg aagcgactcc
    32101 tgggtgcctt cgaccacctc gcgtgcgcgg cgcatccggc ggacaacccc acgcaacatg
    32161 cgcgcgtaag gctggcggct cagcctgaaa taatcctgcc ggtcgcccgg cgtggcggtt
    32221 cgctctatga cacccaactc ctccaacaac cgtgtattcg tgctgatgct cgcgcggctg
    32281 acactcaaac gctcagccag ctcggtcagg ctgaaggggc ctccatgcat gatcatgagg
    32341 cccataatgc ggccagcaat gcgggggaga ccctcaccct ggaggatcag gcccatttgc
    32401 tcaacgaatt caatgactac gtcttcggat ttatctgcca taagtgacaa atagtccttc
    32461 tagtacgttc agtcaatact gaacgaacga gacgcacagg cgtccgatct ggcggaagca
    32521 gggaggcgca cccgtaaagc ctccagggcg ccaagcggcc gcaaaggctg ccaaggggac
    32581 ttgcgacctc tggctggttt actcgcctcc ggcttgcctc tagaaaaact ccaacaaaaa
    32641 cgggccggga cctgatgttc aaaaaactgc tgatcgccaa ccgcggagaa atcgcctgcc
    32701 gtatcatccg aaccgcgaag cggctcggaa ttgagacggt ggctgtctat tcggaggctg
    32761 accgcgacgc cctccatgtc gacatggccg acgaggcggt ggggattggt cccgccgctg
    32821 cggcgcagtc ctatctcgtc atggataaga ttatcgctgc cgccaaggag acgggggccg
    32881 acgcgattca tcccggctac ggcttcttgt cggagaatgc cgcgttcgca aaggcgctga
    32941 agaaggcggg catcgccttt gtcggtccga accctagggc catcgaggcc atgggcgaca
    33001 agatcgagtc caagaagctc gcagccaagg cgaaggtctc gacggtgccg ggccatctcg
    33061 gcgtgatcga ggacgccaag gcggcggtga agattgctga cgacatcggc taccccgtga
    33121 tgatcaaggc gtcggccggc ggcggcggca agggcatgcg catcgcctat agcagcgatg
    33181 aagtcgccga aggcttcgcc tcatcgcagt cggaagcgaa gtcgagcttt ggcgacgatc
    33241 gtgtgttcat cgagaagtac atcgaagagc cgcggcatat cgagattcag gtgcttggcg
    33301 acaagcacgg caatgtcatt catctcggcg agcgtgagtg ctccattcag cgacgcaacc
    33361 agaaagtcat cgaggaggca cctagcccgt tcctcgacga aaagacgcgt gaggcgatgg
    33421 gcgcggaagc cgtcgcgcta tccaaggccg tcgattacga cacggcgggg accgtcgagt
    33481 tcatcgtcga caaggaccgg aacttctatt ttctggagat gaacacccgc ctgcaggtgg
    33541 agcacccggt gacggagctc gtgacggggc tcgatcttgt cgagcagatg ctgaaggtcg
    33601 ccgcgggcga gaagctgtcc atcaagcaga aagacgtgac gctcaccggc tccgccatcg
    33661 agtcgcgcat ctatgcggaa gacccggtgc ggaacttcct gccgtccacg gggcggcttg
    33721 tgcgctttcg tccgcctgaa cttgggaccg aagacggcgt taccatccgc ctcgataccg
    33781 gggtggagga gggcggcgag atctctgtct attacgatcc gatgatcgcc aagctcgtta
    33841 cacatgcgcc gacgcgtatc gaggcgatcg aggagatggg gtgggcactc gatgcgttcg
    33901 ccatcgacgg gttccgccac aacatcccgt tcctcgcggt gctgatgcgg aacgagcggt
    33961 ggcgggaagg gcgtctgtcc accaagttca tcgacgagga atttccggac ggctttcacc
    34021 cgccagtgcc ggagggccac gttcgcgacg tgctggcggc ggttgcagcc agtgtcgact
    34081 atctgtccaa caaccggcgc cgcatgatta cccagcaaat gtctggcgag cccgtgcagt
    34141 tcaccgattc ccgcgtggtt gatcttggcg atcagcctgt cagcgtcggc gtttcgggac
    34201 gcgactatcc catgaccgtg acagtgggcg gcgaggggaa gggcgagaag gcgcgggaga
    34261 tcgtgctcga ctcgccttgg tggtttggcg accccgtttg ggaagggaca gtcgacgacg
    34321 aagaggtggc ggtccaagtg cgtccaatcc tcaacggcgt ccaccttacc tatcaaggcg
    34381 tggaggttcc cgtccgcgtc tacaccgagc gtgagtcggg ctatctggcg ctgatgccgg
    34441 tgaaagaaaa gccggacatg tccaagtatc tactctgtcc gatgccgggg cttgtcgtgt
    34501 cgcttgccgt ggaagaaggc caggacgtgt cggccggcga tacactttgt gtcgtcgagg
    34561 cgatgaagat ggagaacgtg ctcaaggccg agcgcgacgg caccgtttcc aagatcctcg
    34621 ccaagccagg cgacagcctc aacgtggatg ctgtgatcct ggagtttgcc tagggaattt
    34681 gcggtgaaga ccgtgaccgt caggatcgag
//
LOCUS       DBNHVU010000176         7026 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_732095, whole genome shotgun sequence.
ACCESSION   DBNHVU010000176 DBNHVU010000000
VERSION     DBNHVU010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7026)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_732095"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="AC16Y7_07435"
     CDS             <1..303
                     /locus_tag="AC16Y7_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709250.1"
                     /translation="DMGRFRDRVLDLQRHFGQANDDIDKILTSSEKITKRGRKIETLD
                     FDEPATQSEIAPQANGGQPLSHAPVAPQGRQKPAPQPRRETPRARVIRQPDLLPGE"
     gene            300..755
                     /locus_tag="AC16Y7_07440"
     CDS             300..755
                     /locus_tag="AC16Y7_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR5709251.1"
                     /translation="MMLSTMDGSMFDQVIVGGGASLANLIIHALLVGAIVATVHRLRM
                     DQGSIPSFLRYTLVIVATGTLLVAGHFSEVVLWAYTYDWVGAAPPDTDLIYFAFGNYT
                     TLGYGDVVPIPQWHLLGPMTALNGVMLIGWSTALIFEILRRSNPQTQTR"
     gene            complement(679..1665)
                     /locus_tag="AC16Y7_07445"
     CDS             complement(679..1665)
                     /locus_tag="AC16Y7_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR5709252.1"
                     /translation="MIDPIAFFTEPFAYDFMQRAVGVSLLVAAVCAVLSCYLVLKGWS
                     LMGDAISHAVLPGIVIAFVLGIPLVIGAFVAGLACALATGYLKDNSRVKEDTVMGIVF
                     SGMFGFGLVLFTKVETDQHLNHILFGNMLGVTAADLIETAIIAGGTLLIVLAKRRDLL
                     LYCFDPNHARAIGLPVRVLHYGLLVLLSLTIVSSIKAVGIILVIAMLIAPGATAYLLT
                     DRFERMLVIAVVVACTSAVLGTFISFHIDGATGACIVLVQAMAFFAAFLFAPKRGLLA
                     PKGLPSPTPTGGGAPAPHGLFGNPRASLSAFGSGGSSAAKSRRSRPSTSRSA"
     gene            complement(1662..2531)
                     /locus_tag="AC16Y7_07450"
     CDS             complement(1662..2531)
                     /locus_tag="AC16Y7_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR5709253.1"
                     /translation="MSLVLAELLIPFGYDYMWKAMWVSALVGAVCAFLSCYLMLKGWS
                     LMGDALGHAIVPGVAAAYIIGAPFALGAFFAGILAALGMAFVKQHSRLREDAIIGLVF
                     TTMFALGLLMASLYPTSVSIQTIVLGNILAIADEDALQVVIIAVVSFAILLFMWKDLM
                     VTFFDETHAKSIGINTGRVKLIFFTVLSACTVAALQTVGACLVIAMVVTPGATAYLLT
                     DRFSRLIVIAVAFGAVTSFVGAYLSYFVNGATGGVIVTLQTLLFLAAFYFAPKHGVLA
                     ARRKRRAVAEAAA"
     gene            complement(2528..3430)
                     /locus_tag="AC16Y7_07455"
     CDS             complement(2528..3430)
                     /locus_tag="AC16Y7_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese/iron ABC transporter ATP-binding
                     protein"
                     /protein_id="HIR5709254.1"
                     /translation="MSALDLLKQEPAPAPPASIEVRDISVTYPNGFTAVHDASFELCP
                     GTICALVGVNGSGKSTIFKAIMGFVTPSAGEVRLSGLPVEEAVAQNMVAYVPQSEDVD
                     WNFPVLVEDVVMMGRYGHMNMLRVPTREDRRKVDEALERVGMSEYRNRQIGELSGGQK
                     KRVFLARALAQEGRIILLDEPFTGIDVTTETAIIDLLRELREAGRLMLVSTHNLGSVP
                     DFCDQVVLLDRTVLAAGPTAETFTQENLERTFGGVLRNFELGGHALHEDEDSRTLNVL
                     TDDERPLVFYGGKPRKRGEEDKRK"
     gene            complement(3417..4355)
                     /locus_tag="AC16Y7_07460"
     CDS             complement(3417..4355)
                     /locus_tag="AC16Y7_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIR5709255.1"
                     /translation="MKAHWVRRLQGLGAALAILGLSVLAACGEHESSTSTDGKFHVVT
                     TFTVIQDIAQNVAGDAAVVESITKPGAEIHDYQPTPRDIVKTQDADLVLWNGFNLERW
                     FEKFFQNIKDVPSVVVSDGVEPMGITEGPYNGKPNPHAWMSPTSALIYVENIRKALVE
                     HDPKNADVYNKNAAAYAEKIKAMDAPLRKRLSAIPEDKRWLVTSEGAFSYLARDYDMR
                     EAYLWPINADQQGTPQQVRHVIELVRENNLPVVFSESTVSDRPARQVTKESGARYGGV
                     LYVDSLSTEGGPVPTYLDLLRVTVETIAKGFDDERA"
     gene            4538..5833
                     /locus_tag="AC16Y7_07465"
     CDS             4538..5833
                     /locus_tag="AC16Y7_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HIR5709256.1"
                     /translation="MTATGGTTETPAAPERRHWTQILFTKKMLACIFLGFSSGMPLFV
                     LVSLVPAWLRDNGVDLATIGLFALVGLPYTWKFLWSPIMDRFKLPFLGRRRGWALLTQ
                     VLLLLSIGLLGHFNPSTSIQTIVWIVFAVALFSASQDIVIDAYRRELLADDELGTGTS
                     FWINAYRLSGLVPGSLALILADHLPWSTVFWITGAFMLIGIVTTLLVKEVSDDQLAPH
                     TLREAVIDPFVEFFSRDGIKAGLAILIFLFLYKLGDNMATALATPFYLDMGYSKTEIG
                     SVAKFAGLWAVLAGATIGGAAMLKLSINKALWIFGLVQLLTIVPFIWLSQAGHTLAGL
                     FVVVSGEYLAVGLGTVALTAFMARETSKAFTATQFALFSSLIAVPRTVANSTTGFIVE
                     SVGWTQFFIICSLLAIPGMLMLFIVAPWRGKDDKEDEAS"
     gene            5830..6042
                     /locus_tag="AC16Y7_07470"
     CDS             5830..6042
                     /locus_tag="AC16Y7_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709257.1"
                     /translation="MSGATLLWIVGLFAAATVGLLAGVWIGGELEADVNQELLTNADL
                     PEVCWEKIGAEAERIIRDYEGDTPTP"
     gene            complement(6103..6612)
                     /locus_tag="AC16Y7_07475"
     CDS             complement(6103..6612)
                     /locus_tag="AC16Y7_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018009792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709258.1"
                     /translation="MRKRIIVVSDRMQKGYRYELSARVGRNFDPEFTPDLTPREMLRL
                     GVFCGKYMTDSRKEFPKSWFAGAKIAPEGRDCSLNFFGVDASQPLSVWREKGWIHPDD
                     PRGWFQWYCRYYLGRRMPYEDQRQIKRWKAMRRHVRQIERNCEPGDPFCRPRQRQALL
                     HWAYDSRKI"
     gene            complement(6628..>7026)
                     /locus_tag="AC16Y7_07480"
     CDS             complement(6628..>7026)
                     /locus_tag="AC16Y7_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012888.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015016 - heparan sulfate
                     N-sulfotransferase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HIR5709259.1"
                     /translation="EGDGYPFWPFFESIASWWDIRDLPNVMLLHFAELKRNPPSAIRR
                     IAAFLNVPIDEAVFPVIVEHCSFEYMKQNAAKSAPMGGRAWVGGAETFIHKGTNGRWR
                     DVLTPEEIGRYDKIARRELGEPCAAWLAQN"
BASE COUNT         1351 a         2188 c         2189 g         1298 t
ORIGIN      
        1 gacatgggcc gcttccgcga ccgggtgctc gacctgcaac ggcatttcgg ccaagccaac
       61 gacgacatcg ataaaatcct cacctcgtcg gagaagatta ccaagcgcgg acgcaagatc
      121 gagaccctcg acttcgacga gccggcaacg caaagcgaga tcgccccgca ggcgaatggc
      181 ggccagcctc tcagtcatgc gcccgttgcg ccacaggggc ggcaaaagcc ggcgccgcag
      241 ccgcgccgcg agacgccgcg tgcccgcgtc atccggcaac ccgacttact cccgggggaa
      301 tgatgctctc aacgatggac ggctcgatgt tcgaccaggt catcgtgggc ggcggcgcaa
      361 gccttgccaa tctcatcatc cacgcccttc tggtgggcgc catcgtcgcc accgtgcacc
      421 ggctgcggat ggaccaaggc tcgatcccga gcttcctgcg ctacacgctc gtcatcgtgg
      481 ccaccggcac tctgctcgtc gcggggcatt tcagcgaggt ggtactttgg gcctacacct
      541 atgactgggt aggggccgca cccccagaca ccgacctgat ctatttcgcc ttcggcaact
      601 acacgacgct cggctacggc gatgtggtgc ccataccgca atggcaccta ctggggccta
      661 tgacggcgct gaacggcgtc atgctgatcg gctggtcgac ggccttgatc ttcgagattt
      721 tgcggcgctc gaacccccag acccaaacgc gctgaggctt gcccgcggat taccgaacag
      781 gccgtgcggc gccggggcgc cgccgccagt gggcgtgggc gaaggcaaac cttttggcgc
      841 caagagccca cgtttcggcg cgaagaggaa ggcggcgaag aacgccatcg cctggaccag
      901 cacgatgcac gcgcccgtcg caccatcgat atggaagcta atgaacgtgc cgaggacggc
      961 ggaggtacac gccaccacga cggcgatcac aagcatgcgc tcaaaacggt cggtcaagag
     1021 ataggccgtg gcgcctggcg cgatcagcat ggcgatcacc agaatgatgc cgacggcttt
     1081 gatcgagctc acaatggtga gcgagagcag aacgagcaat ccgtagtgca gcacccgcac
     1141 gggcaggccg atggcgcgcg cgtgattcgg atcgaagcaa tagagcaaca gatcgcggcg
     1201 cttggcgagc acgatcagca gcgtgccgcc ggcgatgatc gccgtctcta taagatcggc
     1261 ggcggtcacg cccaacatgt tgccgaatag aatgtggttc aggtgctggt ccgtctcgac
     1321 cttggtgaac aggacgaggc caaagccgaa catgccggag aacacgatgc ccatcaccgt
     1381 gtcttctttc acgcgactgt tgtccttgag gtaaccggtt gcgagcgcgc aagcgagacc
     1441 ggcgacaaac gcaccgatca ccagcggtat ccccagtaca aaggcgatga cgatgcccgg
     1501 caggacggcg tggctgatgg catcgcccat cagcgaccag cccttcagca ccagatagca
     1561 ggacagcacg gcgcagacgg cggccacaag aagcgatact ccgaccgcac gttgcatgaa
     1621 atcgtaggcg aaaggttcgg tgaagaatgc gatagggtcg atcatgcggc ggcctccgcg
     1681 acggcgcggc gcttgcggcg cgcagcaagc acgccatgct tcggcgcgaa gtagaaagcc
     1741 gccaggaaca acagcgtttg cagcgtcacg atcacgccgc ctgtcgcgcc attgacaaag
     1801 taggagaggt aggcgccgac gaaactcgtg accgcgccaa acgcgactgc gatgacaatg
     1861 agtcggctga agcggtcggt caggagataa gccgtggcgc ccggcgtcac caccatggcg
     1921 atgacgaggc aagcacctac ggtctgaagc gccgctacgg tgcaagcgct aagcacggtg
     1981 aagaagatca gtttcacgcg gcccgtgttg atgccgatgc tcttcgcgtg cgtctcgtcg
     2041 aagaacgtca ccatcaagtc cttccacatg aaaagcagaa tggcgaagga cacgacggcg
     2101 atgatgacca cctgcaaggc gtcctcatcg gcgatggcaa ggatgttgcc gagcacgatg
     2161 gtctggatcg acaccgaggt cggatagagc gaggccatga gcaggccgag cgcgaacatt
     2221 gtcgtgaaca cgaggccgat gatggcgtct tcgcggagcc tagaatgctg cttgacgaag
     2281 gccatgccga gcgcggcgag aatgccggcg aagaatgcgc cgagtgcaaa aggcgccccg
     2341 atgatatagg cggcggcaac cccggggacg atggcgtggc ccaacgcatc gcccatcagc
     2401 gaccagcctt tcagcatcaa ataacaggac aggaacgcgc agaccgcacc gaccagcgcg
     2461 ctgacccaca tcgccttcca catataatcg tagccgaacg gaatcaggag ctcggcgaga
     2521 acgaggctca tttgcgcttg tcctcttcgc cgcgcttgcg gggcttgccg ccatagaaca
     2581 ccagcgggcg ctcgtcatcg gtgaggacgt tgagcgtgcg cgaatcctcg tcctcgtgca
     2641 gggcgtggcc gcccagctcg aaattgcgca acacgccgcc gaaggtgcgc tccaggttct
     2701 cttgcgtgaa ggtttccgcg gtcggtcccg cagcgagcac ggtgcggtcg agcagcacca
     2761 cctggtcgca aaagtctggc acgctgccga gattgtgtgt cgagaccagc atcagacggc
     2821 cggcctcgcg caactccctc aggagatcaa tgatcgccgt ctcggtggtg acgtcgatgc
     2881 cggtgaaagg ctcgtcgagc agaatgatgc ggccctcttg ggccagcgca cgggcgagga
     2941 acacacgctt cttctggccg ccggagagtt cgccgatctg gcggtttcgg tactcgctca
     3001 tgccgacccg ctcgagcgcc tcgtcgactt tccggcgatc ctcccgcgtc ggaacgcgaa
     3061 gcatgttcat gtggccatag cggcccatca tcacgacgtc ctcgaccagc accgggaagt
     3121 tccagtcgac gtcctcgctt tgcggcacat aggcgaccat gttctgcgcg acggcctcct
     3181 ccacgggaag gccgcttagg cgcacctcgc cggccgaggg cgtcacgaag cccatgatgg
     3241 ccttgaagat ggtcgacttg ccgctgccgt tgacgccgac cagcgcgcag atcgtgcctg
     3301 ggcagagttc gaacgatgcg tcgtgcaccg cggtgaagcc gttgggatag gtgacgctga
     3361 tgtcccgcac ctcgatgctg gcggggggcg cgggcgcagg ctcttgtttc agaagatcaa
     3421 gcgcgctcat cgtcgaagcc tttggcaatg gtctcgacgg tcacacgtaa cagatcgaga
     3481 taggtcggta ccggcccgcc ttcggtgctc aacgagtcca cgtagagcac gccgccatag
     3541 cgtgccccgg actccttggt gacttgccgt gcggggcggt cggacaccgt gctctcggag
     3601 aacacgacgg ggagattgtt ttcgcggacc agttcgataa cgtgacgcac ctgttggggc
     3661 gtgccttgct ggtcggcgtt gatcggccac agatacgcct cgcgcatgtc gtagtcgcgg
     3721 gcgagatagg agaaggcgcc ttcggaggtg acgagccagc gcttgtcctc ggggatcgcc
     3781 gaaaggcgct tgcgcaaggg cgcgtccatt gccttgatct tctccgcata ggcggccgcg
     3841 ttcttgttgt agacgtccgc gttcttcgga tcgtgctcga cgagcgcttt gcggatgttc
     3901 tccacataga tgagcgcgct cgtgggcgac atccaggcat gcggattggg cttgccgtta
     3961 tacggaccct cggtgatgcc catgggctcc acgccgtcgc tcacgacaac gctcggcacg
     4021 tctttgatgt tctggaagaa cttctcgaac cagcgctcga gattgaagcc gttccacagg
     4081 acgaggtcgg cgtcctgcgt cttgacgatg tcccgcggcg tcggctggta gtcgtgaatc
     4141 tccgcgccgg gcttggtgat cgactccacg accgccgcgt cgcccgcgac attctgggcg
     4201 atgtcctgga tgacggtgaa ggtggtgacg acgtggaact tgccgtccgt ggacgttgaa
     4261 ctctcatgct ctccgcaagc cgcaagcacg ctcagcccaa gaatagccaa tgcggcgccc
     4321 agtccttgga gccgccgaac ccaatgcgct ttcaaacaat gcctccaaat agctgaatta
     4381 cgcccttttc gccttgcagc ttacctgcaa ttgcaaacta ttcgcaagat agattttgac
     4441 gctgcgcatt ggatgctttt cccgggacag gctctttttc cgggagaggc gcttggcgga
     4501 ccatgacggg gccccgagcg gctgataaga ggggcccatg acggctacgg ggggaaccac
     4561 cgaaacaccg gccgcgcctg agcggcgtca ttggacccag atcctcttca ccaagaagat
     4621 gctggcctgc atcttcttgg gcttcagctc gggcatgccg ctgtttgtcc tggtgtcgct
     4681 ggtgccggcc tggctccgcg acaacggagt cgaccttgcc acgatcggcc tgtttgccct
     4741 ggtcgggctg ccttatacgt ggaagttcct gtggtcccca atcatggacc gcttcaagct
     4801 gccgttcttg ggacgccggc gtgggtgggc gctgctgacc caagtgctcc tgctgttgtc
     4861 gatcggcttg cttggccatt tcaatccatc gacctcgatc caaacgatcg tgtggatcgt
     4921 gttcgccgtc gccctgttca gcgccagcca agacatcgtc atcgacgcct accggcgcga
     4981 gttgctggcc gacgacgagc tgggaaccgg cacgtcgttc tggatcaacg cctaccgctt
     5041 gtcgggcctg gtgccgggct cactcgcctt gatcctggcc gaccacctgc cctggtcgac
     5101 cgtattttgg attaccggcg ccttcatgct gatcggcatt gtgaccacgc tgctcgtcaa
     5161 agaagtgagc gacgaccagc tggcgccgca cacgctgcgc gaggcggtga tcgatccgtt
     5221 cgtcgagttc ttcagccgcg atggcatcaa agccggcctc gccatcctca tcttcctgtt
     5281 tctctataag ctcggagaca acatggcgac ggcgctggcc acgcccttct acctggacat
     5341 ggggtacagc aagaccgaga tcggcagcgt ggccaagttc gccggcctat gggccgtgct
     5401 tgctggcgcg acaatcggcg gcgctgccat gctcaagctg agcatcaaca aggctttgtg
     5461 gatcttcggg ctcgtgcagc tcctcaccat cgttcccttt atctggctga gccaggcggg
     5521 gcatacgctt gccgggctgt tcgtcgtcgt ctcaggtgaa tatctcgccg tcgggcttgg
     5581 cacggttgcc ctgaccgcct tcatggcgcg ggaaaccagc aaggctttta ccgctacgca
     5641 gtttgctctg ttctcaagcc tcatcgccgt gccgcgcacg gtggcgaact ccacgacggg
     5701 cttcatcgta gagtccgtgg gctggacgca gttcttcatc atctgctccc tcctggcgat
     5761 tcccggcatg ctgatgctgt tcatagtggc gccttggcgg gggaaggacg acaaagagga
     5821 tgaggcttcg tgagcggcgc tacgctcttg tggatcgtcg gattgttcgc cgccgcaacg
     5881 gtcggccttc tcgccggcgt ttggatcggc ggcgagcttg aagcggacgt caatcaagag
     5941 ttgctgacga atgccgatct gccggaagtc tgctgggaga aaatcggcgc cgaggctgag
     6001 cgcatcatcc gcgactacga aggcgacaca cccacgcctt aagcggggta agctttattt
     6061 ctgcgccagc caggcggcgc aaggatcacg ttcgagaacg actcagatct tgcggctgtc
     6121 ataagcccaa tgcaggagag cctgccgctg cctcgggcgg cagaacggat cgcccggctc
     6181 acagttcctc tcaatctgcc gcacgtgccg gcgcatcgct ttccagcgtt tgatttgccg
     6241 ctggtcttca tacggcatcc ggcggcccaa atagtagcgg caataccatt ggaaccagcc
     6301 gcgcggatcg tcgggatgaa tccacccctt ctcgcgccat acggacagcg gctgactggc
     6361 gtcgacgcca aaaaagttga gcgaacagtc gcggccctct ggggcaattt tggcgccggc
     6421 aaaccagctc ttgggaaatt ccttgcggga gtcggtcatg tacttgccgc aaaatacgcc
     6481 gagccgcaac atctcacgcg gcgtcaagtc gggcgtgaat tccggatcga aattgcggcc
     6541 aacccgggcg ctcaactcat agcgatagcc cttctgcatc ctgtcggaaa caacaatgat
     6601 ccgtttgcgc atggcgagtt cggctgccta gttctgcgct agccaggcgg cgcagggctc
     6661 acccagctcg cggcgagcga tcttgtcgta acggccaatc tcttctggcg tcagcacgtc
     6721 acgccagcga ccattcgtgc ccttatggat gaaagtctcg gcaccgccaa cccaggcgcg
     6781 gccgcccatc ggtgccgact tcgccgcgtt ctgcttcatg tactcgaatg agcagtgctc
     6841 gacgataaca gggaagacgg cctcgtcgat gggcacgttg aggaaggccg caatgcgccg
     6901 aatggcgctg gggggattgc gcttcaactc agcaaaatgg agcagcatca cgttcggcag
     6961 gtcgcgaatg tcccaccagc tcgcgatgct ctcgaagaag ggccagaagg gatagccgtc
     7021 gccctc
//
LOCUS       DBNHVU010000177         3791 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1105092, whole genome shotgun sequence.
ACCESSION   DBNHVU010000177 DBNHVU010000000
VERSION     DBNHVU010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1105092"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="AC16Y7_07485"
     CDS             complement(<1..601)
                     /locus_tag="AC16Y7_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR5709260.1"
                     /translation="MPEPLPPGSTIGILGGGQLGRMLSVAASRLGLKTQIYNDTPDAP
                     AFDVAAASTVGAYEDASLLSDFAKRVDVVTCEFENVPAKALHVAGEIAPVFPPAKAFS
                     VAQDRLAEKDFVSGLRIPVAPYAAVDNLGDLNKALARVGTPALLKTRRFGYDGKGQVA
                     IHTQCEAETALEKIGAAPSLLEGIVPFQRELSVIAVRGQD"
     gene            complement(594..1091)
                     /gene="purE"
                     /locus_tag="AC16Y7_07490"
     CDS             complement(594..1091)
                     /gene="purE"
                     /locus_tag="AC16Y7_07490"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR5709261.1"
                     /translation="MSDENLAVGIIMGSRSDWPTLKATADVLDKLHVPYETKVVSAHR
                     TPNRLYEYASTARERGLKVIIAGAGGAAHLPGMAASMTELPVLGVPVESKALKGLDSL
                     LSIAQMPAGVPVGTLAIGKAGATNAGLLAAGILALGDERLAARLAAWRAEQTAGVHEA
                     VEDNA"
     gene            1251..1676
                     /locus_tag="AC16Y7_07495"
     CDS             1251..1676
                     /locus_tag="AC16Y7_07495"
                     /inference="COORDINATES: protein motif:HMM:NF025607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 domain-containing protein"
                     /protein_id="HIR5709262.1"
                     /translation="MTQDPPASPPTSKLMRFYLLLAVFLIVPIALNYGLAPAETLPKS
                     LDVTVEGTDLTHIFRGLMCLYLGAALFWGIAAFTPSWQRTAVIWVVFFAFSIAVGRVI
                     SLIVDGRPSALLLIYLGLEIAAGLLGLFILSVADRKSKR"
     gene            complement(1666..1872)
                     /locus_tag="AC16Y7_07500"
     CDS             complement(1666..1872)
                     /locus_tag="AC16Y7_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HIR5709263.1"
                     /translation="MESEEIRELRTVLERLKQEHRDLDSAIVALEDSGRADALQLKRL
                     KKKKLFLRDEISRVEDQLLPDIIA"
     gene            2066..2242
                     /locus_tag="AC16Y7_07505"
     CDS             2066..2242
                     /locus_tag="AC16Y7_07505"
                     /inference="COORDINATES: protein motif:HMM:NF016231.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HIR5709264.1"
                     /translation="MALDAHLTELSDRHRALDQKIEQEQGRPTSDEFKIAEWKRQKLR
                     LKDEMERLKHEPRH"
     gene            2471..2845
                     /locus_tag="AC16Y7_07510"
     CDS             2471..2845
                     /locus_tag="AC16Y7_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5709265.1"
                     /translation="MQTAWRAGALALALFAAGAGSSAVAAEKGLANKGEVLVRENCAR
                     CHAVALVGAGPNAEAPPFRTLGRKYPIENLAESLAEGIVSGHPEMPIFVFKPHDVDAI
                     LAYLESIQEASQADPEDEALRQ"
     gene            complement(2858..3235)
                     /locus_tag="AC16Y7_07515"
     CDS             complement(2858..3235)
                     /locus_tag="AC16Y7_07515"
                     /inference="COORDINATES: protein motif:HMM:NF023390.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIAA family protein"
                     /protein_id="HIR5709266.1"
                     /translation="MLSYSEHDNAQAVEICLSGRVSTEEFDRVAKKLEAFIARHGRVR
                     ILEQIKDFEGMDAAALWHDVQFSFRHLKDFNRIAVVANPEVHNLWSGLVSPFIECDVE
                     HFAPDQIEEARNWLMWPEGAADA"
     gene            3591..>3791
                     /locus_tag="AC16Y7_07520"
     CDS             3591..>3791
                     /locus_tag="AC16Y7_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709267.1"
                     /translation="MSQEPPVKIKTEAPVKRTLAYQQALTQPREEPKKDAETPDIPAF
                     ETVTTEDGTTPAGDPSAIQDIPA"
BASE COUNT          684 a         1145 c         1218 g          744 t
ORIGIN      
        1 cgtcttgccc ccgcacggcg atcaccgaga gctcgcgctg gaacggcacg atgccttcca
       61 acagggacgg cgcggcgccg atcttctcta aggccgtttc ggcctcgcac tgtgtgtgga
      121 tcgcgacctg tcccttgccg tcatagccga accggcgcgt cttgagcagg gcaggggtgc
      181 ccacccgtgc gagcgccttg ttgagatcgc cgagattgtc tacggcggcg taaggcgcaa
      241 ccggaatcct aagcccgctg acgaagtcct tctctgcgag tctgtcttgc gcaaccgaaa
      301 aggccttggc cggcggaaac acgggggcaa tctccccggc cacgtggagt gctttggccg
      361 gcacgttctc gaactcgcaa gtgacgacgt cgacacgctt cgcaaagtcg gacaacaggc
      421 tcgcgtcttc gtaggcgccg acggtggacg ccgccgcgac gtcgaaggcg ggtgcgtccg
      481 gcgtgtcatt gtagatttgt gttttgaggc cgagccggct ggcagcgaca gagagcatgc
      541 ggccgagctg gccgccgccg agaatgccga tcgtgctgcc cggcggtagc ggttcaggca
      601 ttatcttcga cagcctcgtg cacgcctgcg gtttgttccg cgcgccaggc ggcgagccgg
      661 gcggcgaggc gctcatcacc gagcgcgaga atgccggccg cgaggagccc ggcattggtg
      721 gccccagcct tgccaatggc aagcgtgcca acgggtacgc cggccggcat ctgggcgatg
      781 gacagaagac tgtcgagacc cttcagcgcc ttgctctcca cgggcacgcc gagcacggga
      841 agctccgtca tggatgcggc catgccgggc agatgcgccg cgccgccagc gcccgcgatg
      901 atgactttca gcccccgctc gcgtgcagtc gaggcgtact cgtaaagccg gtttggcgta
      961 cggtgggcgg agaccacctt ggtctcgtag ggcacgtgta gtttgtcgag gacgtccgcg
     1021 gtcgctttta gcgtgggcca gtccgagcgg cttcccataa tgatgccgac ggcgaggttc
     1081 tcatcgctca tgctttcgct catgggaagg tccggcgcat ggcctgttcg cggaaagcgc
     1141 gcgattatag ggacgcgcac cttcttggca aggcgagact cgccgctgtc cccttgcggt
     1201 ttcaccagga atcggttgcc ctgagacccc gactacgcga tgaggacgcc atgacccaag
     1261 atccgcctgc ctcccctccg accagcaagt tgatgcggtt ctacctgctc ttggccgtct
     1321 ttctgatcgt gccgatcgcg ctcaactacg gacttgcgcc cgctgagacg cttcccaagt
     1381 ccctcgacgt cacggtcgag ggcacggacc tgacccatat cttccgcggg ctgatgtgcc
     1441 tttatctcgg ggcggcgctg ttttggggca ttgccgcgtt cacgccgtcc tggcagcgca
     1501 cggccgtgat ctgggtcgtc ttcttcgcgt tctcgattgc ggtcggacgg gtgatcagcc
     1561 tcatcgtgga cggccggccg agcgcgcttc tgctcatcta tctgggactt gagatcgcgg
     1621 ctggcttgct gggcctgttt attctgagcg tggccgacag aaagtctaag cgatgatgtc
     1681 gggcaggagc tgatcctcga cccgggagat ctcgtctctc aaaaagagct tcttcttctt
     1741 aagacgcttg agttgaaggg cgtcggcgcg accagagtcc tccaacgcga cgatggcgct
     1801 gtccaggtcg cgatgctcct gcttgagccg ttccagcacc gtccgaagct cgcgaatctc
     1861 ctcgctctcc atcgggcgct tttactccat tgccttcacg gcagaaagta tcggagcgaa
     1921 agcggggcgg tgcaagggtt tgggggcccc agcgcggttt ccacagatgc gtgtgccgcg
     1981 cgcctctatc aacagcccgg cgttagacaa gcgcagtgta gtttgtcaga atgattgtgg
     2041 tgacaatcga ctttggaggt ccggtatggc gctagacgca cacctcactg aattgtctga
     2101 caggcaccgc gcgttggatc agaaaatcga gcaagaacaa ggaaggccaa cgtcggacga
     2161 gttcaaaatc gctgaatgga agcgtcagaa actacgtcta aaagatgaaa tggagcggct
     2221 gaaacacgag ccgcgacact agacgaggcc tccagcctca gatttgaggc caggctttag
     2281 gtgatcgatg tccgcgacac ctggtttgtt ccgctccagg tggataaggg tgttttatgg
     2341 tcgtgatcga gaggctccag gccgctcccg cggccattgt tgtttgtagg gccctcgttg
     2401 atttggatca gtgtggaagc cgtcgggcgc catgatcttg aacgctcaga acaagggagg
     2461 ccgggacatc atgcagacag cttggcgcgc aggcgcattg gcgttggcat tgtttgctgc
     2521 gggcgcgggg tcttcagcgg tggccgccga gaaaggctta gccaacaaag gcgaggtgct
     2581 cgtccgggag aattgcgcgc gctgccatgc cgtcgcgctg gttggcgccg gcccgaacgc
     2641 ggaagcgccg ccgttccgca cgctcgggcg caaatatcca attgagaatc tcgccgaatc
     2701 gctcgctgaa ggaatcgtct ccggccatcc ggagatgccg atcttcgtgt tcaagcccca
     2761 cgacgtggac gcgatccttg cctatctgga gtcgattcaa gaggcgtcgc aggccgatcc
     2821 cgaagacgag gctttgcgcc aataaaggcc gcgccgttca ggcgtcggcg gcgccttccg
     2881 gccacatcag ccaattccgg gcttcctcga tttggtcggg ggcgaagtgc tccacgtcac
     2941 actcgataaa gggggacacc aggcccgacc agagattgtg gacctccgga ttggccacga
     3001 cggcgatgcg gttgaagtcc ttgaggtggc ggaagctgaa ttggacgtca tgccacagag
     3061 cggcggcgtc catgccttcg aaatccttga tttgctcgag gatacgcacg cggccgtggc
     3121 gcgcgatgaa ggcctccagc ttcttcgcca cgcggtcgaa ttcctccgtg gagacccggc
     3181 cgctgaggca aatttcgacg gcttgggcgt tgtcgtgctc ggagtaggac agcatggagg
     3241 cggggccggg gtttttagcg ggctgttcga tgtttcgccg aaatggccgc catcttagcc
     3301 gtcaaggcgg agggctgcct agcgtttggg gtgggcttaa tctgctgcgc cggcgcggcg
     3361 cggtgtcact tgccacacca gctgtccaca gggcacggtt ggggcggtta gttcaccgga
     3421 atttaagctt aacgaaccga caataccggc gtaatacgga cctaaagaca ctcaatcgcc
     3481 gccttttttc ggattactat gggcgtcaat agaggagacc ggcgatcaag aagctgctct
     3541 tttatctcgt ctgcactctc accatcgttg ttggcggggt gtggatgctc atgagccagg
     3601 agccccccgt caaaatcaag acggaggccc cggtcaagcg cacgctcgcc tatcaacagg
     3661 cgctcaccca gccgcgcgaa gagccgaaga aggacgccga gacgccggac atcccggcct
     3721 tcgagaccgt caccaccgag gacggcacca cgccagccgg cgacccgtcg gccattcagg
     3781 atatccccgc c
//
LOCUS       DBNHVU010000178         5941 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_657604, whole genome shotgun sequence.
ACCESSION   DBNHVU010000178 DBNHVU010000000
VERSION     DBNHVU010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5941)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5941)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5941
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_657604"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="AC16Y7_07525"
     CDS             <1..241
                     /locus_tag="AC16Y7_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR5709268.1"
                     /translation="GPVCETGDYLALARPLPELEAGDLIAVMTAGAYGAVQASQYNSR
                     LLVPEVLVSGAEYCVTRPRPDYNDMLKAERLPGWL"
     gene            350..3088
                     /locus_tag="AC16Y7_07530"
     CDS             350..3088
                     /locus_tag="AC16Y7_07530"
                     /inference="COORDINATES: protein motif:HMM:NF025152.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02302 family protein"
                     /protein_id="HIR5709269.1"
                     /translation="MAQRNRQKSSPGDPKDDPEAASLKRRFESRVRLSWLALFAERAW
                     EALLWPFVVVCAFLVFTLLGGWSLLPPLVHRIVLLGFGTGLIAACLPLIRIALPTRAE
                     ALRRLERNAEVPHRPASSYEDTLSAEPPTETVTLWTLHRQRLSQLIAKLKPSWPAPRA
                     DRKDPFAIRAALFIGLIAAFIAAGPDARSRLDAAFSPAASASPKLIRLDAWVTPPVYT
                     GMAPLVLADGSEAIGRGAESFRALEVPERSQLIVRTHAPEGETVTLTIYNEDSSEAKT
                     IEPKTGGSTGLVEFNVGLRKPSTADVRISGSTVAQWQFALTEDAAPQIALRGEPTATP
                     RGALRLEYSALDDYGVASAEANFTLAPDQEDALGAAFPVEGIEEEEDLVESEPLFGPP
                     VMKLQLPRSNAKKVEGRGTQDLTAHPWAGLKVRMTLAAKDQAEQTGKSEVSEFVLPAR
                     VFTKPLARAVIEQRRKLVQEPDSSAMVARALDGLTIGDEKAIDDRVVYLSLRNTFWRL
                     RNDDSRDAVSSVVAQLWSTALRIEEGDLPEAERELKSAQDALLRALQENAPAEEIKKR
                     IEELRAALSRYLQALAAQQQEKGNMAAESQQDGDQLVSEQDLDKMLENIQDLAEAGSK
                     DMAERMLSELNDILDRLQTGNFPENAQQQRAQKMMKDLDDVVSDQQELLDDTFAEKRE
                     QRQGQGGGQSEQFDVSPPGQPMEFAPGMSMAPFSEGMPQGGEGGPRAQGQSGRQGDAP
                     GGGQPQIGQQQGQPQGPLGDLKKRQEALRNKLEQLIERMGMAGNDSPGEFEGASEAME
                     EAEQAISERNLDRATQSQSLALDRMRQGAESMAQQMTQDGQEQAGGQGPGSNGRDPLG
                     RPDRSNRPDQGLSVQVPDEIDIQRAREILDELRARIGDPTRPMLELDYLERLIRSF"
     gene            3201..4355
                     /locus_tag="AC16Y7_07535"
     CDS             3201..4355
                     /locus_tag="AC16Y7_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018095682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2333 family protein"
                     /protein_id="HIR5709270.1"
                     /translation="MLNSIVNFFTATFRAIGRGIGLGIAFMLRPFVWLGRWFAQRGIL
                     LKVLLGLILVGFLALYGYFFWNTQVWSGFDTNYADRYEHTSDTGLSNAKPSGGGIITG
                     ESETQDAETATQTCRKSMVVESTADLVDFNVNENAWISSMLLYKLGLFGLPWESTPFF
                     DNKAAFQRGVHQAVRRTAIELVDTLGRVRGTSQIDKDLQSARGALQFDETTWYFSFSP
                     FGPKTPTPSFYRSAIKDLHKFNDRLKKCEAVFDVRADNLLQFVDRIAKDIGATSAILR
                     ERSENYSSGWFDPRADDRFWFAYGQLYAYYGILTAARTDFADVIEERRLTALWDELEE
                     QLRASLRIQPAIISNGAEDGWLMPSHLATLGFYVLRVRSNLVEVRLVLDR"
     gene            complement(4352..4897)
                     /gene="hpt"
                     /locus_tag="AC16Y7_07540"
     CDS             complement(4352..4897)
                     /gene="hpt"
                     /locus_tag="AC16Y7_07540"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR5709271.1"
                     /translation="MAEQPVGETVRPLFTAETIATRIDAMARDIAACDLHDPIIVAVL
                     KGSFIFAADLIRALSRQGLHPEIDFIFLASYGSGTTGGEVKILRDVESTIEDRDVVIV
                     DDILDSGRTLSFATAHLAGRGARGVKTCILLNKQVPRGVDVTPDFTGFECPDLFVVGY
                     GMDLAHCYRELPFIGVVEPRV"
     gene            complement(4940..>5941)
                     /locus_tag="AC16Y7_07545"
     CDS             complement(4940..>5941)
                     /locus_tag="AC16Y7_07545"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HIR5709272.1"
                     /translation="APPPPQAAPPAPPPEPPAPPEPAPPPPPPQAAPLPPPPPPPQAA
                     PPVPPQGDVEFGTDEALADQVARINAEAGVPGAEGVPPAPEPEAEEPEKKGGLFGKLM
                     RKRDEPAPPPPPPPADMAPPPPPSDMAPPPQEQEEVSFGAPQDSSLDASFDAGFPEEG
                     GAQDPALEDPNLVAAAFGESVAKATTPKKRPPIVAIGWGILGLIVLTVLGLFVFAPAT
                     TVSILPGASRIYAMLGMPVGAEGLAFEGVRYGWTTNAGQTVLEVQGNVRNTGSSVMAV
                     PTVVIALRDEKGEEISEWTTEVGAAELSAGEEAPFLKQIPSPPSNVRSLKVRFADAK"
BASE COUNT         1082 a         1973 c         1910 g          976 t
ORIGIN      
        1 tggcccggta tgcgagaccg gcgattatct ggcgctggcc cggccgcttc ccgaactgga
       61 ggcaggcgac ctcatcgccg tgatgaccgc aggcgcttat ggcgcggtgc aggcctccca
      121 gtacaacagc cgtctcttgg tacccgaagt gttggtgtcg ggggccgaat attgcgtcac
      181 ccggccccgg ccggactata acgacatgct caaggccgag cgcctgcccg gctggctcta
      241 aagcctataa tcggccctga aatgcgcctg tatttttgcc aggttcagcc cccaaattac
      301 cgtgatgtaa ccttcggcca ggcgctgcct gtgctaccaa ttccggacca tggcccaacg
      361 caaccgccaa aaatccagcc ccggcgaccc caaggacgat cccgaggccg cctcactcaa
      421 gaggcgcttc gagagccgcg tccgtctgag ctggctggcg ctgttcgccg agcgcgcctg
      481 ggaggcgttg ctctggccgt tcgtcgtggt ctgcgcgttc ttggtgttca cgctgctcgg
      541 cggctggagc ctgctgccgc cgctcgtcca tcgcatcgtt ctgctcggct tcggtaccgg
      601 gctcatcgcc gcttgcctgc cgctcatccg gattgccttg ccgacgcgcg ccgaggccct
      661 gcggcgcctt gagcgcaacg ccgaggtccc gcaccgcccg gcctcgtcct atgaagatac
      721 gctgagcgcc gaaccgccca ccgagacggt gacgctgtgg acgctccatc gtcagcgcct
      781 gtcgcaactc atcgccaagc tgaagccctc ctggcccgcc ccgcgcgcgg accggaagga
      841 tccttttgcg atccgagcgg cgctgttcat cggtcttatc gccgcgttca tcgccgccgg
      901 ccccgatgcc agatcccgcc tcgatgcggc cttctcgccg gccgccagcg cttcccccaa
      961 gctgatcagg ctcgatgcct gggtgacgcc gccggtctat accggcatgg cgccgctcgt
     1021 tctggccgac ggcagcgaag cgatcggccg gggcgccgaa tccttccgcg cactggaagt
     1081 gcccgagcgc agccagctca tcgtccgcac gcacgcgccc gaaggcgaga ccgtgacgct
     1141 gaccatctac aacgaggaca gcagcgaggc gaaaaccatc gagccgaaga ccggcggcag
     1201 caccggactg gtcgagttca atgtcggctt gaggaagccc tccaccgccg atgtgcgcat
     1261 tagcggatcg accgtcgctc aatggcaatt tgccctgacc gaagacgccg cgccgcagat
     1321 tgccttgcgg ggcgagccga ccgcgacgcc ccgtggcgcg cttcgcttgg agtatagcgc
     1381 cctcgacgat tacggcgtgg ccagcgccga agccaacttc acgcttgcgc cggaccaaga
     1441 ggacgcactt ggcgccgcct tccccgtcga aggcatcgag gaagaagaag atctcgttga
     1501 gtcggagcct ttgttcggcc cgccggtgat gaagctgcaa ctgccgcgct cgaacgccaa
     1561 gaaggtcgag ggccgcggca cccaggacct cacggcgcat ccttgggctg ggctcaaggt
     1621 gcgcatgaca cttgccgcaa aagatcaggc cgaacagacc ggcaagagcg aggtgagcga
     1681 gttcgttctg ccggcgcgcg tcttcaccaa gccgctcgcg cgcgccgtga tcgagcagcg
     1741 ccgcaagctc gtgcaggaac cggattcgtc cgcgatggtc gcccgcgcac tggacggact
     1801 gaccatcggc gatgagaaag cgatcgacga tcgcgtcgtc tatctgtctt tgcgcaatac
     1861 gttctggcgt ctcaggaacg acgactcgcg tgacgcggtc tcaagcgtcg ttgcccagct
     1921 gtggtccacc gcgctgcgca tcgaggaagg cgatctgccg gaagccgagc gcgaactgaa
     1981 gagtgcgcag gacgcattgt tgcgcgcgct tcaggagaac gcaccggccg aggagatcaa
     2041 gaagcgcatc gaagagcttc gcgccgcgct gtcgcgctac ctacaagcgc tcgccgccca
     2101 gcagcaagaa aagggcaaca tggccgccga gagccagcag gacggtgacc agctcgtctc
     2161 ggagcaggat ctcgacaaga tgctcgagaa catccaggac ctggccgaag ccgggtcgaa
     2221 ggacatggcc gagcgcatgc tgtcagagct gaacgacatt ctcgatcgtc tgcagaccgg
     2281 caacttcccg gagaacgccc agcagcagcg cgcccagaag atgatgaaag acctggacga
     2341 cgtggtctcc gatcagcagg agctgttgga cgacacgttc gctgagaagc gtgagcagcg
     2401 gcaaggtcaa ggcggcggcc agagcgagca gttcgacgtg agcccgcccg gccagcccat
     2461 ggagttcgcc cccggcatga gcatggcccc cttctctgaa gggatgccgc aaggcggcga
     2521 gggcggcccg agggcgcaag gccaatcagg ccggcagggc gatgcgccgg gcggcggcca
     2581 accccagatc gggcagcaac aagggcagcc gcaaggcccg cttggcgatc tcaagaaacg
     2641 ccaggaagcg ctgcgcaaca aactggagca gctcatcgag cgcatgggca tggcggggaa
     2701 cgactcgccg ggcgagttcg aaggcgccag tgaggccatg gaagaggccg agcaggcgat
     2761 cagtgagcgc aacctcgacc gcgcgaccca gagccaaagc cttgcgctcg accgtatgcg
     2821 ccagggcgcg gaatccatgg cgcagcagat gacgcaggac ggccaagagc aggcgggcgg
     2881 ccaaggccca ggctccaatg gccgcgaccc gctcggccgc ccggaccgtt cgaaccggcc
     2941 cgaccaaggc ttgagcgtcc aagtccccga cgagatcgac atccagcggg cgcgcgagat
     3001 tcttgacgaa ctgcgcgcgc ggatcggcga cccgacacgt cccatgctcg agctcgacta
     3061 tttggagcgg cttatccgct ccttctaggc gccattctcg cttttcgaaa aaagtgcgtg
     3121 cggccacagc aacgccgtcg tcgatgccct atatgtcgcc ctactatggt gtggaaccgt
     3181 cgaaagggct ttgaggcgcg atgctgaact ccatcgtcaa tttcttcaca gcgacttttc
     3241 gggctatcgg ccgcgggatc gggctcggca tcgccttcat gctgaggcca ttcgtttggc
     3301 tcggccgctg gttcgcccag cgcggtatcc tcctcaaagt gctgctcggg ctgatccttg
     3361 tcggcttcct cgccctttac ggctacttct tctggaacac gcaagtctgg agcggcttcg
     3421 atacgaacta tgcggaccgc tatgagcaca caagcgacac cggcttaagc aacgcaaagc
     3481 cgagcggcgg cggcatcatc accggagaga gcgaaaccca agacgccgag accgccacgc
     3541 aaacatgccg taagtccatg gtggtggagt ccacggccga cctggtcgac ttcaacgtga
     3601 acgagaacgc gtggatctcg tccatgctgc tctacaagct cggcctgttc gggcttccct
     3661 gggagagcac gcccttcttc gacaacaagg ccgccttcca gcgcggcgtg caccaagccg
     3721 tccgccggac cgcgatcgaa ctcgtcgaca cgctggggcg cgtacgcgga acgtcgcaaa
     3781 tcgacaagga tttgcagagc gcgcgcggcg cgctccaatt cgacgaaacc acctggtatt
     3841 tttcgttcag tcccttcgga cccaagacgc ccacgccgag cttctaccgc tccgcgatca
     3901 aggacctgca caagttcaac gacagactta agaagtgcga ggccgtgttc gacgtgcgcg
     3961 ccgacaatct gctgcaattc gtcgaccgca ttgccaagga catcggcgcc acctcggcga
     4021 tcctgcgtga gcgttcggag aactacagca gcggctggtt cgacccccgc gccgacgacc
     4081 gcttctggtt cgcctacggc cagctctacg cctattacgg cattctcacc gccgcgcgca
     4141 ccgactttgc cgatgtcatc gaggaacgcc gcctgacggc cctatgggac gagctcgagg
     4201 agcaactgcg ggcctcgctt cgtattcagc ccgcgatcat ctccaacggc gcggaggatg
     4261 gctggctcat gccctcgcat ttggcgacgc ttggcttcta tgttctgcgt gtgcgctcca
     4321 acctcgtgga ggtacggctg gtcttggacc gttaaacgcg cggctccacc acgccgatga
     4381 acggcaactc gcgatagcag tgcgcgaggt ccatgccgta gcccaccacg aacaggtcgg
     4441 gacattcgaa cccggtaaag tcgggcgtga cgtcgacacc gcgcggcact tgcttgttca
     4501 gaaggatgca ggtcttcacg ccgcgcgcac cgcggcctgc gagatgcgcc gtggcaaagg
     4561 aaagcgtgcg gccagaatcg agaatgtcgt cgacgatcac cacgtcgcgg tcttcgatcg
     4621 tgctctccac gtcacgcaga atcttgacct ctccgcccgt ggttccgctg ccgtagctgg
     4681 ccagaaagat gaagtcgatc tccgggtgca gtccttggcg tgagagcgcg cggatcagat
     4741 cggcggcgaa gatgaagctg cccttcagca ccgcaacgat gatcggatcg tggagatcgc
     4801 aggcggcaat gtcgcgcgcc atcgcatcaa tacgggttgc gatcgtctcg gccgtgaata
     4861 ggggcctgac cgtttccccc acgggctgct ccgccatgct gatttggccc tgctaacttg
     4921 gccttctcga tcggccgtcc tatttggcgt cggcgaaacg gaccttgagg cttcggacat
     4981 tgctcggcgg cgaagggatc tgcttcaaaa acggcgcctc ctcaccggcg gaaagctcgg
     5041 cagcaccaac ctccgtcgtc cactcggaga tttcctctcc cttctcgtcg cgcagcgcaa
     5101 tgaccacagt cgggacggcc atgacggacg agcccgtatt gcgcacattg ccttggactt
     5161 cgagcacggt ctgtccggca ttggtggtcc agccatagcg aacgccttcg aaggcgaggc
     5221 cctcggcgcc caccggcatg ccgagcatcg cgtagatgcg cgaggcgccc ggcaggatcg
     5281 atacggtggt ggccggcgcg aacacgaaca agccgagcac ggtcagcacg atcaggccca
     5341 aaatgcccca gccgatggcg acgatgggcg gccgtttctt cggcgtcgtg gctttggcca
     5401 ccgattcgcc aaaggcggcg gcaaccagat tggggtcttc cagcgccgga tcctgtgcac
     5461 cgccctcctc cgggaagccc gcatcgaacg atgcgtccag ggaactgtct tggggcgcgc
     5521 cgaaggacac ttcctcctgc tcttggggcg gaggcgccat gtcggagggt ggaggcggcg
     5581 gcgccatgtc ggccggcggt ggaggcggcg gcggggcagg ctcgtcgcgc ttgcgcatga
     5641 gcttcccaaa cagcccgccc ttcttctccg gttcctcggc ctcaggctca ggcgccggcg
     5701 gcacgccttc agcacccggt acgccggcct cggcattgat gcgcgcgacc tgatcggcga
     5761 gcgcctcgtc ggtgccgaac tcgacatcgc cttgaggcgg caccggcgga gcggcctgag
     5821 gtggcggcgg cggggggggc agcggagcag cctgtggagg aggaggcggt ggcgcaggct
     5881 caggcggtgc cggcggctcg ggcggcggtg ccggtggggc agcctgcggc ggaggcggag
     5941 c
//
LOCUS       DBNHVU010000179         3920 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1254726, whole genome shotgun sequence.
ACCESSION   DBNHVU010000179 DBNHVU010000000
VERSION     DBNHVU010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1254726"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..3410
                     /gene="carB"
                     /locus_tag="AC16Y7_07550"
     CDS             3..3410
                     /gene="carB"
                     /locus_tag="AC16Y7_07550"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR5709273.1"
                     /translation="MVGAGPIVIGQACEFDYSGTQACKALKEEGYRIVLVNSNPATIM
                     TDPDVAHATYIEPITPDVLTKIIEKERPDALLPTMGGQTALNTALSLDRSGVLKAYGV
                     EMIGARAPVIDKAEDRELFREAMTKIGLETPRSVLADAGEVKSADRAKYKAEIARIET
                     EFPDGEARAKAKLDFEADWGRHESERRRRYVEKGLIEGLEGLQEIGLPAIIRPSFTLG
                     GTGGGIAYNREEFIEIVERGLDASPTSEVLIEESVLGWKEYEMEVVRDKDDNCIIICS
                     IENIDPMGVHTGDSVTVAPALTLTDKEYQIMRNASIAVLREIGVETGGSNVQFAVNPE
                     DGRLVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDELANDITGGATPASFE
                     PTIDYVVTKIPRFAFEKFSGSTPTLTTSMKSVGEAMAIGRSFQESLQKALRSMETGLT
                     GLDEFHFEGLGQGDDKNVIREALGSPTPDRLLKVAQALRLGVDQEQIYDSCRVDPWFL
                     RQLQDIVDAEARVREHGLPNSAAQFGALKAQGFSDERLATLAGLETAEVAERRRALDV
                     HPVYKRIDTCAAEFASPTAYMYSTYEAGFPGGESCEARPSAKDKIIILGGGPNRIGQG
                     IEFDYCCCHAAFALSDAGFETIMVNCNPETVSTDYDTSDRLYFEPLTEEDVLEIVRKE
                     QENGTLKGVIVQFGGQTPLKLAAALEKAQVPILGTSPDAIDLAEDRDRFKALIENLGL
                     RQPNNGIARSAEEAVAIAKEIGFPVVIRPSYVLGGRAMEIVHDEAQLQRYIAEAVVVS
                     GNSPVLLDSYLRDAIEVDVDALADGKDVFVCGIMEHIEEAGVHSGDSACSLPPHSLKP
                     EIVADLERQAVELARALNVVGLMNVQFAIQHDDIYILEVNPRASRTVPFVAKVVGLPI
                     AGLASEVMAGKPIASFGLESPTFEHIAVKEAVFPFARFPGVDPILGPEMRSTGEVMGI
                     DRDYAVAFAKSQLGGGAKLPLSGTVFVSVKDTDKDRLLSPMRTMVEMGFRIVATRGTK
                     RHLDAHGIPCERVNKVLEGRPHIVDAITNGQIDIVFNTTEGAKALADSMSIRRSALLN
                     HIPYYTTLAGALAVTGAIKALREGSLTVAPLQSYVGQGPKKGLAETA"
     gene            3502..>3920
                     /gene="greA"
                     /locus_tag="AC16Y7_07555"
     CDS             3502..>3920
                     /gene="greA"
                     /locus_tag="AC16Y7_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR5709274.1"
                     /translation="MDKVPMTAEGYAALEAEIKTLKTVERPRIIKLISEARSHGDLSE
                     IAEYHAAKELQGITEARMADLEDKLSRADIIDVSKLKGDQVMFGATVTLVDEDTAEKV
                     KYRIVGEVEADVKEGRISITSPIARALLGKRKKDVVEV"
BASE COUNT          787 a         1252 c         1240 g          641 t
ORIGIN      
        1 tgatcgtcgg ggccggaccg atcgtgatcg gccaggcctg cgagttcgac tattcgggca
       61 cgcaagcctg taaggcgctg aaggaagagg gctaccgcat cgtcctggtg aattcgaacc
      121 cggcgacgat catgaccgac ccggacgtgg cccacgccac ctatatcgag ccgatcacgc
      181 cagacgtgct caccaagatc atcgagaagg aacggcccga tgcgttgctg cccaccatgg
      241 gcgggcagac ggcgctgaac accgcgctca gcctcgaccg ctcaggcgtg ctgaaggcct
      301 atggcgtgga gatgatcggc gcacgcgcgc ccgtaatcga caaggccgag gaccgcgagc
      361 tgttccgtga ggcgatgacc aagatcggcc tggagacgcc gcgctcggtg ctggccgacg
      421 cgggcgaggt caagagcgcc gaccgcgcga aatacaaggc cgagattgcc cgcatcgaga
      481 ccgagtttcc agacggcgag gcccgcgcca aagccaagct ggacttcgag gccgattggg
      541 gacggcacga aagcgagcgg cgcaggcgct atgtggagaa aggcctgatc gagggcctcg
      601 aagggcttca ggagatcggt ctgccggcca tcatccgccc ctcctttacg ctgggcggca
      661 ccggcggcgg cattgcctat aaccgcgagg agttcatcga gatcgtcgaa cgcggcctgg
      721 atgcctcgcc gaccagcgaa gtgctgattg aagaatcggt gctcggctgg aaggagtacg
      781 agatggaggt cgtccgcgat aaggacgaca actgcatcat catctgctct atcgagaaca
      841 tcgacccgat gggcgtgcac acgggcgact cggtcacggt ggcgccggcg ctgacgctca
      901 ccgacaaaga ataccagatc atgcgcaacg cctcgatcgc ggtgctgcgc gagatcggcg
      961 tcgagaccgg cggctcgaac gtacagtttg ccgtcaatcc ggaagacgga cgcctcgtcg
     1021 tcatcgagat gaacccgcgc gtgtcgcgct cttctgcctt ggcgtcgaag gcaaccggct
     1081 ttcctatcgc caagatcgcg gcgaagctcg cggtcggata cacgctggac gaactcgcca
     1141 acgacattac cggcggcgcc acgcctgcct ccttcgagcc aaccatcgac tatgtggtga
     1201 ccaagatccc gcgcttcgct ttcgagaagt tctccgggag cacgccgaca ctcaccacct
     1261 cgatgaagtc ggtgggcgag gccatggcca tcggccgcag cttccaggag tcgctacaaa
     1321 aggcgctgcg ctccatggag actggactga cggggctcga tgagtttcac ttcgagggtc
     1381 tcggccaggg cgacgacaag aacgtcatcc gcgaggcgct gggctcgcca acgcccgacc
     1441 ggctgctaaa agtcgcgcaa gccttgcgcc ttggcgtcga ccaggagcag atctacgaca
     1501 gctgccgcgt cgatccctgg ttcctccgcc agcttcaaga catcgtcgat gcggaagccc
     1561 gtgtacgcga acatggattg ccgaacagcg ccgcacagtt cggtgcactc aaagcgcaag
     1621 gcttctccga cgaacggctg gcaacgctgg cagggctaga gacggcggag gtcgccgaac
     1681 gccgccgcgc gctcgacgtg catcccgttt acaaacgcat cgacacgtgc gccgcggaat
     1741 tcgcctcgcc caccgcttat atgtactcaa cctacgaggc gggcttcccg ggcggggagt
     1801 catgcgaagc gcggccgtcc gcgaaagaca agatcattat cctgggcggc ggaccgaacc
     1861 gcatcggcca aggcatcgag ttcgactatt gctgctgcca cgccgcattc gcgctgtccg
     1921 atgcggggtt cgagaccatc atggtcaact gcaatccgga gaccgtctcc acggactacg
     1981 acacgtccga ccggctctat ttcgagccgc tcacggaaga agacgtgctg gagatcgtgc
     2041 ggaaggaaca agagaacggc acacttaaag gcgtcattgt gcagttcggc ggccagacgc
     2101 cgctgaagct cgccgcggcg cttgagaagg cgcaagtgcc gatcctcggc acgtcgcccg
     2161 acgcgatcga cctggccgaa gaccgcgacc gcttcaaggc gctaatcgag aacctaggcc
     2221 tcaggcaacc gaacaacggc atcgcacgct cggcggaaga agccgtcgcc atcgccaagg
     2281 agatcggttt ccccgtggtg atccgcccgt catacgtgct tggcgggcgc gccatggaga
     2341 tcgtgcacga cgaagcgcag cttcagcgct acatcgccga ggccgtggtg gtctcgggta
     2401 acagcccggt gctgctcgac tcttatttgc gcgacgccat cgaagtggac gtcgacgcac
     2461 tcgccgacgg caaagacgtg ttcgtgtgcg ggatcatgga acatatcgaa gaagccggcg
     2521 tgcattccgg cgacagcgcc tgctcgctgc cgccgcactc cttgaagccg gagatcgtcg
     2581 ccgacctgga acgtcaggcg gtggagctgg cccgcgcgct aaacgtggtc ggcctgatga
     2641 atgtgcagtt cgccatccag catgacgaca tctacattct cgaagtgaac ccgcgcgcct
     2701 cgcgcaccgt accgtttgtg gccaaggtgg tcgggcttcc tattgctggc ctcgcctcgg
     2761 aggtcatggc gggcaagccg atcgcctcat tcgggctgga gagcccgacg ttcgagcata
     2821 tcgcggtgaa ggaagccgtg tttccgttcg cgcgcttccc cggcgtcgat ccgattctgg
     2881 ggccagaaat gcgctccacc ggcgaggtca tgggcatcga ccgggattac gcggtcgcct
     2941 tcgccaagag ccagctcggt ggcggcgcca agctgccgct ctccggcacg gtgttcgtct
     3001 cggtcaagga caccgacaag gaccggctcc ttagccccat gcggaccatg gtggagatgg
     3061 gcttccgcat cgtcgccacg cgcggcacga agcggcatct cgacgcgcat ggcatccctt
     3121 gcgagcgggt caacaaggtg ctggaggggc gcccgcatat cgtcgacgcc atcaccaatg
     3181 gccagatcga catcgtgttc aacaccaccg agggcgccaa ggccctggcc gattccatgt
     3241 ccatccgccg gtcggccttg ctcaaccaca ttccctacta tacaaccctc gctggcgcgc
     3301 tggccgtgac cggggccatc aaggccctcc gggaaggcag cctgaccgtc gctcccctcc
     3361 agagctatgt cggccaaggc cccaagaagg gtctcgccga gacagcctag gtaaagacgg
     3421 tcaaaatcaa cagattcttg acttgggcgg gtctattcct cgacagtatt cagggggcct
     3481 cccttaggag ctttgactga tatggataag gtgcccatga ccgcggaggg ctatgccgcc
     3541 ctcgaagccg agatcaagac gctcaagacc gtcgaacggc cgcggatcat caaattgatt
     3601 tctgaagccc gctctcatgg cgacctctcg gaaatcgccg agtaccacgc cgccaaggag
     3661 ctgcagggca tcaccgaggc ccgcatggcg gaccttgagg acaagctgtc gcgcgccgac
     3721 atcatcgatg tgtccaagct caagggcgat caggtcatgt tcggcgcgac cgtcacgctg
     3781 gtcgacgagg acaccgccga gaaggtcaag taccgcatcg tcggcgaggt cgaggccgac
     3841 gtgaaggagg gcaggatctc catcacctcg ccaatcgcgc gagcgcttct cggcaagcgc
     3901 aagaaggacg tggtcgaggt
//
LOCUS       DBNHVU010000180         7765 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1179318, whole genome shotgun sequence.
ACCESSION   DBNHVU010000180 DBNHVU010000000
VERSION     DBNHVU010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7765)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7765)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7765
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1179318"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /gene="sdhC"
                     /locus_tag="AC16Y7_07560"
     CDS             complement(<1..197)
                     /gene="sdhC"
                     /locus_tag="AC16Y7_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HIR5709275.1"
                     /translation="MSNKLEAERPLSPHLQIYRPMLTMMMSIAHRISGAALAVGFALL
                     TWWLVAVSMGPGAYDCVQSFFG"
     gene            complement(328..477)
                     /locus_tag="AC16Y7_07565"
     CDS             complement(328..477)
                     /locus_tag="AC16Y7_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709276.1"
                     /translation="MDGPYEGDWGGTAYVIGSHVWETVDGGNTWILSGFTDDMNAGFE
                     IATPA"
     gene            complement(578..1084)
                     /locus_tag="AC16Y7_07570"
     CDS             complement(578..1084)
                     /locus_tag="AC16Y7_07570"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HIR5709277.1"
                     /translation="MELKARVAAAALALSAIASPALAADTATAVLKDPEGKDVGAVTL
                     RAVPTGVLLDTKLSGFPPGDHAFHIHGTGKCEPPSFKSAGGHFNPEEDEHGLENSAGP
                     HGGDMPNIHVPESGELHIEVLNQMVSLGGLLGGEGTAIVVHQKGDDYKSNPAGDAGPR
                     IACGVIAE"
     gene            1204..2109
                     /gene="yegS"
                     /locus_tag="AC16Y7_07575"
     CDS             1204..2109
                     /gene="yegS"
                     /locus_tag="AC16Y7_07575"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF009602.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HIR5709278.1"
                     /translation="MTKKRAAICLILHKKSCTRPEVKAAVKHVRQSVDFEVYIPWRGK
                     DLRRFVRQAIADGAQRIVAGGGDGTLNAVVNAMLRKGKAPKASLGILPLGTANDFARG
                     AGIAAGDLISALELACSGKPTSIDIGSMNGQYFINVASGGFGAEITATTPQDMKKALG
                     GAAYSIMGFVKAFQLQPYKGRLILADGAVKEGSMLVMAVGNNRFAGGGYDVAPKACLT
                     DGLLDIAVVSGERPERLGDLTEELKDPTSPKNKHLFYRQLPSFTIETDRPLHVNLDGE
                     PIQSRRLEFRACPKALSVVLGTASA"
     gene            complement(2106..3179)
                     /locus_tag="AC16Y7_07580"
     CDS             complement(2106..3179)
                     /locus_tag="AC16Y7_07580"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR5709279.1"
                     /translation="MTDTTDKIDRTITGNYFEDFTLGQEIVHATPRTVTVGDVSVYTA
                     LYGGRFAVQSSDAFAQGIGYPRSPIDDMLVFHFVIGKTVPDISRNAIANLGYAEFRFE
                     TPVYPGDTIASVSEVIGVKQNSSGKNGTVYVRTTGRNQKGEVVLSFKRWVMVVKRDLD
                     LPAPETVIPELDKEVQPDSFGKALPPLKLTVYDYALAGSPRRWEDYQVGEKIDHVDGQ
                     TIEEAEHQMATRLYQNTARVHFNQHRESQERFGRRLVYGGHIISIARSLSFNGLANAF
                     HVLGLNGARHAAPSFAGDTIYAWSEVLDKAELPGRSDMGALRLRTFAVKDRNCADFPG
                     VKDDGKLEEGVVLDMDYWVALPR"
     gene            complement(3176..3493)
                     /locus_tag="AC16Y7_07585"
     CDS             complement(3176..3493)
                     /locus_tag="AC16Y7_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5709280.1"
                     /translation="MSWALLVLAGLFEVGFASTLKLTENFTKLWPTLIFTVCIILSFV
                     LLNRALKDIPIGTAYAVWTGIGAAGTAMVGILVYKEPATALRLFFIATLIGSIIGLKL
                     ATK"
     gene            complement(3719..3808)
                     /gene="pqqA"
                     /locus_tag="AC16Y7_07590"
     CDS             complement(3719..3808)
                     /gene="pqqA"
                     /locus_tag="AC16Y7_07590"
                     /inference="COORDINATES: protein motif:HMM:NF019654.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HIR5709281.1"
                     /translation="MKEWMKPEVTESEAGMEVTSYLPAELDRA"
     gene            complement(4032..4121)
                     /gene="pqqA"
                     /locus_tag="AC16Y7_07595"
     CDS             complement(4032..4121)
                     /gene="pqqA"
                     /locus_tag="AC16Y7_07595"
                     /inference="COORDINATES: protein motif:HMM:NF019654.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="HIR5709282.1"
                     /translation="MKEWLKPEITESEAGMEVTSYLPAELDRA"
     gene            complement(4306..4443)
                     /locus_tag="AC16Y7_07600"
     CDS             complement(4306..4443)
                     /locus_tag="AC16Y7_07600"
                     /inference="COORDINATES: protein motif:HMM:NF047966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter small subunit"
                     /protein_id="HIR5709283.1"
                     /translation="MSEPTEQENVSGANTIKLALAWSFVGIPLIWGVLNTLSKAALLF
                     Q"
     gene            complement(4443..5846)
                     /locus_tag="AC16Y7_07605"
     CDS             complement(4443..5846)
                     /locus_tag="AC16Y7_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate MFS transporter"
                     /protein_id="HIR5709284.1"
                     /translation="MATAAQADSWSLLHWLDRSHTVAKPGFSRWLVPPAALCVHLCIG
                     QVYAFSVFNLPMTKLIGITESAPDDWKLTELGWIFSIAIVFLGLAAAFTGTWLDRVGP
                     RKAMVTAALCFGGGFLISALGVYLHELWIVYLGYGVIGGCGLGLGYISPVKTLITWFP
                     DRPGMATGMAIMGFGGGAMIASPLSVWLMDYFSSPTDVGVTGTFITMGLIYMFFMLVG
                     ATIVRVPAEGWAPEGYVAPAESKKMVTTAHVHVDEALKTPQFYLLWGVLCLNVTAGIG
                     VLGQASAMSQEMFPGKITAAAAAGFVGLLSLFNMIGRFFWASTSDYIGRRNTYMIFFA
                     LGMALYAAVPWTGETGSIILFVAFYCIIMTMYGGGFATIPAYLRDVFGVRYVGAIHGR
                     LLTAWSTAGVLGPVLVNYIREYQINNGVPKADAYSVTMYIMAGLLLVGFLLNWLMTPV
                     DKKHHMTEAELKASMEG"
     gene            6146..6784
                     /locus_tag="AC16Y7_07610"
     CDS             6146..6784
                     /locus_tag="AC16Y7_07610"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HIR5709285.1"
                     /translation="MPLPKSLLEGYRRFRSGRYAQDEARYRKLAGGQSPGAMVIACCD
                     SRVDVSAIFDTDPGDLFVMRNVANLVPTFEPDSGLHATSAAVEYAVTALNVPNVIVLG
                     HSQCGGVAAYRQTLKGKADETRFIGRWLTVLDGLDVHKGDLDAFSEETAFELAGVRQS
                     LANLRSFDALKEREAAGLLKLHGLHFDIGSGTVLALDEKADSFAPLPEDGCA"
     gene            complement(6781..>7765)
                     /locus_tag="AC16Y7_07615"
     CDS             complement(6781..>7765)
                     /locus_tag="AC16Y7_07615"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR5709286.1"
                     /translation="GREMLNILAEREFPADEVIPLASRRSIGVEVSYGDNRLKCRDLE
                     NFDFAGVDFALMSAGSTISKEWSLRIGAQGCVVIDNSSFWRYDPDVPLIVPEVNADAI
                     AGFTKKNIIANPNCSTAQLVVVLKPLHDAAKIKRVVCSTYQSVSGAGKDAMDELWNQT
                     KGIFVTEPPEPEKFTKQIAFNVIPHIDVFLDDGSTKEEWKMVAETKKILDPKIKLTAT
                     CVRVPVFVGHAEAVNIEFETPFSTEEAREVLREAPGVLVVDKREPGGYVTPVECVGDY
                     ATFVSRIRDDQTVDNGLSLWCVSDNLRKGAALNTIQIAEAMIERDLLKKAA"
BASE COUNT         1540 a         2292 c         2442 g         1491 t
ORIGIN      
        1 ccgaagaagc tctgaacgca atcataggcg ccggggccca tgctgaccgc gactagccac
       61 caggtcagaa gtgcgaagcc gacggccagc gccgcgccag agatgcgatg cgcgatcgac
      121 atcatcatgg tcagcatcgg ccgatagatt tgcagatgcg gggagagcgg acgctctgcc
      181 tctagcttat ttgacattgg ttattagcgc gtgttgccca gtcgagtcct ttgaatagct
      241 cagtcaacgg gcaggggcaa tcgggcggac ctgcttcctt tggtctgggc ccggcgcgct
      301 ctttcggatc ttcttcgtgg gtgaaggcta agcgggcgtc gcgatttcga atccggcgtt
      361 catatcgtcc gtgaatccgg acaggatcca ggtgttgccg ccatcgactg tttcccagac
      421 atgcgagccg ataacatagg ccgtgccgcc ccagtcgcct tcgtacgggc cgtccatgct
      481 ttaagtcccc caacgcagta cgaatcgtga ggctcagcat ggtgcccaaa tatttgacag
      541 aaagtttaat tcgggaggcc tggggcctct tcgccggcta ttcggcgatg acaccgcaag
      601 cgatgcgggg cccggcgtcg ccggccgggt tcgacttata gtcgtcaccc ttctggtgga
      661 cgacgatggc ggtgccttcc ccgccgagta ggccgccgag gctcaccatc tggttgagca
      721 cctcgatatg aagctcgccc gattcgggca catggatatt cggcatgtcg ccgccatggg
      781 gccccgcgga attctcaagc ccgtgctcgt cttcttccgg attgaaatgc ccgcccgccg
      841 acttgaagct cggcggctcg cacttgcccg tgccgtggat gtggaatgcg tggtcgccag
      901 gcggaaagcc gctcagcttg gtatcgagca agacacccgt tgggacggcc cgcagcgtca
      961 cggcgcccac gtccttgccc tccgggtcct tcaggacagc cgttgccgtg tcggcggcaa
     1021 gcgcaggcga ggcgatggcg ctcagagcca aggcagccgc tgcaaccctt gctttcagtt
     1081 ccatgggaga cactcttgtt ctcatcgtcc aacaactccc gtcgatgcag tagacttaga
     1141 acatgacaca cgtgtggccg ttggaacaga cagcctgcac atcgcctcgg gaaaggaatg
     1201 tcgatgacca agaagcgcgc agcgatctgc ctcatcctcc ataagaagtc ctgcacacgg
     1261 cctgaggtca aggcggcggt caagcacgtt cggcagtcgg tagacttcga ggtctacatt
     1321 ccgtggcgtg gaaaggacct ccgccggttc gttcggcaag caattgccga tggtgcacag
     1381 cggatcgtag cgggcggcgg tgacggcacc ctgaacgccg tcgtcaacgc gatgctacga
     1441 aagggcaagg cgccgaaagc atcgctcggg atactgcctt tggggaccgc caatgacttt
     1501 gcaaggggtg cgggcattgc agcgggcgac ttgataagcg ctcttgagtt ggcctgttca
     1561 ggaaagccga ccagcatcga tattggctcg atgaacggac agtatttcat caacgtcgcc
     1621 agcggaggct ttggggccga gatcaccgcg acgacgcccc aggacatgaa gaaggccctt
     1681 ggcggcgcgg cctattccat catgggcttt gtcaaggcat tccagcttca accttataag
     1741 ggccggctga tcctcgccga cggtgcggtg aaggaaggat cgatgctggt catggcggtc
     1801 ggcaacaacc ggttcgctgg tggcggctac gacgtcgcgc caaaggcttg tctaacggac
     1861 gggctgctcg acattgctgt cgtgagcggc gaaaggcccg aacggctcgg tgatctgact
     1921 gaagaactaa aagatccgac aagccccaag aacaagcacc tgttctatag gcaactgcca
     1981 tcgttcacga ttgagacgga cagaccgctc cacgtgaacc ttgacggcga gcccatccag
     2041 agtcgtcgtt tagaattcag agcttgcccg aaagccctgt ccgtcgtgtt gggaacggct
     2101 agcgcctagc gtggcagggc cacccaatag tccatgtcga gcacgacgcc ctcttcgagt
     2161 ttgccgtcgt ccttgacgcc cgggaagtcc gcgcaattgc ggtccttcac ggcgaaagtg
     2221 cgcaggcgca gcgcgcccat gtcggagcgg cccggaagct cggctttgtc gagcacttcg
     2281 ctccaggcgt agatggtgtc gccggcgaaa gacggcgcgg catggcgcgc gccattcagc
     2341 ccgagaacat ggaaggcgtt ggccagcccg ttgaagctca acgaacgagc aatcgagatg
     2401 atatgcccgc catagacgag gcggcgaccg aagcgctctt gggattcccg gtgctggttg
     2461 aaatgcacgc gggccgtgtt ctggtaaagc cgcgtcgcca tctggtgctc ggcttcctcg
     2521 atggtctggc cgtccacatg gtcgatcttc tcgccgacct ggtagtcctc ccagcggcgt
     2581 gggcttccgg ccagcgcgta gtcgtagacc gtgagcttca gcggcgggag cgccttgccg
     2641 aagctgtcgg gctgcacctc tttgtcgagt tcgggaatga cggtttccgg cgccggtaag
     2701 tcgaggtcgc gctttacgac catcacccag cgcttgaagc tcagcaccac ctcgcccttc
     2761 tggttgcgcc ccgtggtgcg cacatagacg gtgccgttct tgccgctgga gttctgcttg
     2821 acgccgatca cctcggatac ggacgcgatc gtgtcgcccg ggtagacggg cgtctcgaag
     2881 cggaactcgg catagccaag attggcgatg gcgttgcgcg agatgtccgg cacggtcttg
     2941 ccgatgacga agtggaacac gagcatgtcg tcgatcggcg agcgcggata gccgatgcct
     3001 tgggcaaagg cgtccgagga ctgcacggcg aaccggccgc cataaagggc cgtgtagacg
     3061 ctcacatcgc cgacggtcac cgtgcgcggg gtcgcgtgca cgatctcctg gccgagcgtg
     3121 aaatcctcga aataattgcc ggtgatggtc cggtcgatct tgtctgtggt gtcggtcatt
     3181 ttgttgcgag tttcagtccg atgatggagc caataagcgt ggcgatgaag aacaggcgca
     3241 acgccgtggc cggctcctta tagacgagga tgccgaccat ggcagtgccc gcggcgccga
     3301 ttcccgtcca cacggcgtag gccgtgccga tggggatgtc cttcagcgcc cggttgagga
     3361 gcacgaagct caagataatg cagactgtga atatcaaggt tggccagagc tttgtgaagt
     3421 tctctgtcag cttcagcgtg gaggcgaagc cgacctcgaa tagtcctgca agcacaagca
     3481 aggcccatga catggggtca cctctgggtc gcaccatttc gagttggcgg ggaggcggcc
     3541 cgtttaagga cgaaagcgcg cctgacgagg cggaccataa attcgggagg ggtctctgtc
     3601 accccaccgg atgcggtgac gggcaggggt gcgtctaaag cgtgcgtcaa aaacgaatgg
     3661 cggccccgct attcgctggg gccgccattg cagcaaaaca aaaaggcttc gcgtctaatt
     3721 aggcgcggtc gagctcagcc ggaagatagg aggtaacctc catgccagct tcggattcgg
     3781 taacctcagg cttcatccac tctttcatga tgcgtctcct tttctaaagt tgctcactga
     3841 atatgggcct tggcgatgag aaccgcatga agcgctcatg aacgcaatct gacagccaga
     3901 ttagcggaat ctgacaggat gtcgcagggg ggaggcgccg aaggcgggac ggatggcgag
     3961 cgcccaaacg aaaatggcgg ccccgctatc gctgggaccg ccatttcgaa aacttgctag
     4021 atcagcgcga attaagcgcg gtcgagctca gccggaagat aagaagtcac ttccatacca
     4081 gcttcggact cggtgatctc gggcttcagc cactctttca tgatcgtctc cttcggtgaa
     4141 cttcaagacg agataatggg gagctaggat gagaaccgca tgactcgcgc attaacagat
     4201 tctgaataga ataatccttg tagaacaatg cactagctga attaccgcta ccaaaccggc
     4261 tgctgaacgg gccagattca cgccagattc atgggctcaa cgtcgctact ggaacagcag
     4321 ggccgccttg gacagggtgt tgagcacgcc ccagatgagg ggaatgccga caaaggacca
     4381 ggccagggcg agcttgatcg tattggcgcc ggagacgttc tcctgttcgg ttggctcgct
     4441 catcatccct ccattgaggc tttgagttcc gcctcggtca tgtggtgctt cttatccacg
     4501 ggggtcatca gccagttcag caggaagccc accaggagca acccggccat gatgtacatg
     4561 gtgaccgaat aggcgtctgc tttgggcacg ccgttattga tctggtactc gcggatgtag
     4621 ttcaccagca cgggcccgag gacgccggct gtggaccagg cggtcaagag acgcccatga
     4681 atggcgccca catagcggac gccgaagaca tccctgagat aggcggggat ggtggcgaag
     4741 ccgccgccat acatggtcat gatgatgcag tagaaggcga cgaacaggat gatcgagccg
     4801 gtctcgcccg tccagggcac ggcggcgtag agcgccatgc ccagcgcaaa gaagatcatg
     4861 taggtgttgc gccggccgat atagtccgag gtcgaggccc agaagaagcg gccgatcatg
     4921 ttgaataggc tcaacaggcc gacaaacccg gccgccgccg ccgccgtgat cttgccgggg
     4981 aacatctcct gactcatggc ggaggcctgg cccagcacgc caatacccgc cgtcacgttc
     5041 aagcagagca cgccccacag gaggtagaat tgcggggtct tcagcgcttc gtccacatgg
     5101 acatgggcgg tggtgaccat cttcttggac tcagccggcg ccacatagcc ctcaggtgcc
     5161 catccttcgg caggtacgcg cacgatggtg gcgcctacca gcatgaagaa catgtagatg
     5221 agccccatgg tgatgaaggt tcccgtgacg cccacatcgg tggggctgga gaagtagtcc
     5281 atcagccaga ccgaaagcgg cgaggcgatc atcgcgccgc cgccgaagcc cataatggcc
     5341 ataccggtgg ccatgcccgg ccggtccggg aaccaggtga tcagtgtctt caccggcgag
     5401 atgtagccga ggccgagccc gcagccgccg atcacgccat agccgagata gacgatccaa
     5461 agctcgtgca gatagacgcc gagcgcggag atcagaaaac cgccgccgaa gcacagggcc
     5521 gcggtgacca tggctttgcg cgggccgacg cggtcgagcc aggtgcccgt gaacgccgcc
     5581 gccagcccga ggaacacaat ggcgatggag aagatccagc cgagctcggt cagcttccag
     5641 tcgtcagggg cggactcggt gatgccgatg agtttggtca tcggcaggtt gaacacgctg
     5701 aaggcgtaaa cctggccaat gcacaaatgc acgcacaagg cggcgggcgg cacgagccaa
     5761 cggctgaagc cgggcttggc cacggtgtgc gatcggtcca gccagtgaag aaggctccaa
     5821 gaatccgcct gcgctgcggt cgccatgcgt caaatccccc ctagaacatc gttttatgcg
     5881 cttgctgcgc ttgttggccg gtagtctggc cgggctttgc gcatatcgca agggctcttg
     5941 gcgcgccaat cttactcgcg ccggttgacc ggcgcgttcc gtgcgatcat gacagggtgg
     6001 tgacaggtgc agccatacgg acgcatcgcc ataatcggtt gcgccgctca gttctgcccc
     6061 aaagtcctgc tggacgctga cggcttgggc tggaatcttg aagcgtttga ttatctgagg
     6121 atttgtcttg ttttgaggat ttgtcttgcc cctaccaaaa tcacttcttg aaggatatcg
     6181 acgtttccgc agcggccgct atgcgcagga cgaagcgcgt taccggaaac tcgctggtgg
     6241 ccagtcgcct ggggccatgg tgatcgcctg ctgcgattcc cgcgtggacg tctcggcgat
     6301 tttcgatacg gacccgggcg atctgttcgt catgcgcaac gtcgccaacc tcgtcccaac
     6361 gtttgagccg gacagcggtc tccatgccac tagcgcggca gtggaatatg ccgtcaccgc
     6421 gctcaacgtg ccgaacgtca tcgtgctcgg ccattcgcaa tgcgggggcg ttgccgccta
     6481 tcgccaaacg ctcaagggta aagccgacga gacgcgcttc atcggccgct ggctcacggt
     6541 cctcgatggg ctcgatgtgc ataagggcga tctcgacgcg ttcagcgagg agacagcgtt
     6601 cgagctcgcc ggcgtccggc agtcgctcgc taatttgcgc agcttcgatg cgttgaaaga
     6661 gcgcgaggcg gcgggcctgc tcaaactgca cgggctccac ttcgatattg gcagcgggac
     6721 ggttctggcg ctcgacgaga aagccgatag tttcgcgccg cttcctgaag acggctgcgc
     6781 ttaagcggcc ttcttcagca ggtcgcgctc gatcatggcc tcggcgatct ggatcgtgtt
     6841 gagcgccgcg cctttgcgca aattatccga gacgcaccaa agcgacaggc cattgtcgac
     6901 ggtctggtcg tcgcgaatgc ggcttacgaa cgtcgcgtag tcgccaacgc attccacggg
     6961 ggtgacatac ccgcccggct cgcgcttatc gaccacgagc acgcctggtg cctcgcgtaa
     7021 gacctcgcgc gcttcctcgg tggagaacgg cgtctcgaac tcgatgttaa ccgcttcggc
     7081 gtggccgaca aacaccggca cgcgcacgca tgtcgcggtc agcttgatct tgggatcgag
     7141 gatcttcttg gtctcggcca ccatcttcca ctcttccttg gtcgagccat cgtcgaggaa
     7201 gacgtcgata tgcgggatga cgttgaaggc gatctgcttg gtgaacttct ccggctcggg
     7261 aggttcggtg acgaaaatcc ccttggtctg attccacagc tcgtccatgg cgtccttgcc
     7321 cgcgccagag acggattggt aggtggagca gaccacgcgc ttgatcttcg cggcgtcgtg
     7381 cagcggcttc agcaccacga cgagctgggc cgtcgagcaa ttcggattgg cgatgatgtt
     7441 cttcttggtg aagccggcga tggcgtcggc attcacttcc ggcacgatca acggcacgtc
     7501 cgggtcatag cgccagaacg acgagttatc gatgaccacg caaccttgtg ctccgatccg
     7561 cagcgaccat tccttggaga tggtggagcc cgcggacatc agcgcgaagt cgacgcccgc
     7621 aaagtcgaag ttctccaggt cgcggcactt caggcggttg tcgccgtaag agacctccac
     7681 gccgatggag cggcgcgagg cgagcggaat gacctcgtcg gcggggaatt cccgctcggc
     7741 cagaatattt agcatctcgc gcccg
//
LOCUS       DBNHVU010000181         8066 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1477579, whole genome shotgun sequence.
ACCESSION   DBNHVU010000181 DBNHVU010000000
VERSION     DBNHVU010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1477579"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1769)
                     /locus_tag="AC16Y7_07620"
     CDS             complement(<1..1769)
                     /locus_tag="AC16Y7_07620"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: protein motif:HMM:NF014732.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HIR5709287.1"
                     /translation="MSTSKTLPAADIDLATLGHALWRAKRWILGLTLLAGAITFIGLS
                     MMRPLYTSEARILVQNEDSAFTRPTGEDGRNGYRTPLDEQAVQSQVQVLTSRDLILQV
                     VKDLDLVHNATFQRDAGQTFLRRLLDAIGIGSKRQSAQERAANALADNLDVFQLSKSS
                     VIAVEYSSGDPKLAAEIANKLADVYIGWQRTAKIEQTKDATAWLADQIKALRKATTES
                     EEAVEKFKASKGLYAGAGNVTLNAQQLSELNSQLILSEAQESEARARAKLIKQMLADN
                     GDINATPEVPKSQLIINLIEQRVEVQRQLAELSATLLPSHPRIQQLRSELTDVRTQIR
                     NEAGKVVKSLENEAQVAAAREASLRASLNAAKSKSAGQSDAEIRLRALEREAKANRDL
                     LESYLARYRDASARHDMGAVPAQAAIVSRAHASVLPSFPKKVPLSLLVMAATALLAVG
                     YTLASELIQGAPAQEYVPAPQAKPSRRAKRKRSAAAKASANAPEPRQAATAGSSIPEV
                     RRRKPARTTTATPAASVAPERKLPMVIAASPSATPKTDETASTPPRWQAKKPAKRPSW
                     LKINEKQGQEPTSQAAPQPVPEPT"
     gene            1920..2282
                     /locus_tag="AC16Y7_07625"
     CDS             1920..2282
                     /locus_tag="AC16Y7_07625"
                     /inference="COORDINATES: protein motif:HMM:NF014606.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HIR5709288.1"
                     /translation="MARFATRLGLLLLVSLIAGLTGCKGGGVPGLTTPDFALSTPAPL
                     PAPGEAYPAGAGRAPPGVPVIPNETYTLDSGDSVRIIVFGQQNLSNVYSVDGSGAVAM
                     PLIGPVRARGLTTFELSS"
     gene            2331..2579
                     /locus_tag="AC16Y7_07630"
     CDS             2331..2579
                     /locus_tag="AC16Y7_07630"
                     /inference="COORDINATES: protein motif:HMM:NF046672.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HIR5709289.1"
                     /translation="MEVATYRLFFILGEVNRPGQYPYVSAMTVEAAAAIAEGYTERAK
                     KRYVRLTRRFGGVMSTVMVPTDYPVQPGDTIYVRERFF"
     gene            2622..3794
                     /locus_tag="AC16Y7_07635"
     CDS             2622..3794
                     /locus_tag="AC16Y7_07635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5709290.1"
                     /translation="MSSNFRILHCLRAPVGGLFRHVHDLALGQAEMGAEVGIVCDSRT
                     GGGSDLDVALSRLKDNCALGVTRIPMARTPGFNDWRVYRKIAKLARKLDVDVLHGHGA
                     KGGAYARLAGRGLRKKKGTKVVYTPHGGVLHYSPSALSGRLYLALERKLGPMTDGLIF
                     ESVYTGNRYVELVGPPVCPVRVIVNGLHRHEFYESLLADDAADFVFVGELRKLKGVDV
                     FLEALAAHQTIFPGRAIIVGSGPDEKHFKRMARKLGLTSRVMFSGPQPASTAFVRARC
                     VVVPSRAESLPYIVLEAAAAQMPMIASNVGGIPEIVGDVPMPLIPPGDVDALAGQLRA
                     FLADPNPFLRRAEELQRHVAQQFTVEAMTRDTVDFYISELGAGIHKLPELSAETTS"
     gene            3941..4435
                     /locus_tag="AC16Y7_07640"
     CDS             3941..4435
                     /locus_tag="AC16Y7_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709291.1"
                     /translation="MAAVCAVGVLVAAQSIPKVAEIVGQRASFEQSYDVGPQGRFGGQ
                     RKAVELVATHPLGIGALEFGRVHHVEDVHQVYLAMYLNAGWIGGTLYLFLVLATIMLG
                     LRLVLRDRGGTGISAVLVAAFIGMALEGAVIDTDHWRHFYLVMAMIWGMALVAPRAVR
                     DNWS"
     gene            complement(4449..4526)
                     /locus_tag="AC16Y7_07645"
     tRNA            complement(4449..4526)
                     /locus_tag="AC16Y7_07645"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4489..4491),aa:Pro,seq:ggg)
     gene            complement(4607..5059)
                     /locus_tag="AC16Y7_07650"
     CDS             complement(4607..5059)
                     /locus_tag="AC16Y7_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015950812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR5709292.1"
                     /translation="MEKAPEAFRTISEVADELEVPKHVLRFWEAKFAQLKPMKRGGGR
                     RYYRPEDVALLRGIRFLLYNDGYTIRGVQKILREEGPRYVMDYRRLAEMSGDDEIAAP
                     YIPDIAAEAAQGPGVYRPGSAPNGDTLGALLEELEACHRLLADADTKD"
     gene            complement(5091..5408)
                     /locus_tag="AC16Y7_07655"
     CDS             complement(5091..5408)
                     /locus_tag="AC16Y7_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HIR5709293.1"
                     /translation="MSGKTLTRADLAEAVFQKVGLPRNESAEIVELVLREIVTSLERG
                     DPVKLSSFGSFGIRDKGERIGRNPKTGQEVPITPRRVLVFRASNIMKQRINEALSRSK
                     AAE"
     gene            complement(5545..6522)
                     /locus_tag="AC16Y7_07660"
     CDS             complement(5545..6522)
                     /locus_tag="AC16Y7_07660"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007611077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR5709294.1"
                     /translation="MSTIRSVIKGVGGYLPARVVTNADLEALVDTSDQWITARTGIRK
                     RHIVADGELTSSMGAAAAKAALADAGLEPDDIDLIILATSTPDQTFPASAVTIQAELG
                     ITHGAAFDVQAVCSGFVFALSTADNYLKSGQFNRALVIGAEAFSRILDWEDRSTCVLF
                     GDGAGAVVVEAQKLNGVDTERGILATCLRSDGRYRDKLYVDGGPSSTGTVGHLRMDGP
                     EVFRHAVTKISQVITETLGKAGYEAADIDWFVPHQANKRILDGAARKLGLAPERVVIT
                     VDKHANTSAASIPLALTEAYTDGRIKSGDLVLLEAMGGGFTWGALLIRW"
     gene            complement(6540..7583)
                     /gene="plsX"
                     /locus_tag="AC16Y7_07665"
     CDS             complement(6540..7583)
                     /gene="plsX"
                     /locus_tag="AC16Y7_07665"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR5709295.1"
                     /translation="MGDPVTIALDAMGGDHGPSVVIPAAAIALVRHPPMRFILVGDEA
                     KIVAELAAYPQLAERSEVVHTDVAIAMDAKPSQALRRGRWKSSMWLAIQAVRDGKADA
                     VVSAGNTGALMAMSKFCLKTVKGIDRPAIAAIWPTVAAECIVLDVGANVGADSRQIVE
                     FALMGAAMARALFGMERPSVGLLNIGVEEVKGVEQIKEAAQVLREAPLPIRYHGFVEG
                     DDIGHDVVDVVVTDGFTGNIALKTAEGTAKQIGEYLRAAMGRSLLARLGAILAQGAFR
                     ALKEKMDPRQLNGGIFLGLNGIAVKSHGGTDATGFASAVDLAYEMASSGVVKRLGSDV
                     AQFHTKLELEGAA"
     gene            complement(7695..>8066)
                     /locus_tag="AC16Y7_07670"
     CDS             complement(7695..>8066)
                     /locus_tag="AC16Y7_07670"
                     /inference="COORDINATES: protein motif:HMM:NF014657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR5709296.1"
                     /translation="IHLSGRLTADFTQTCVVSLEPLPVAVDIPVEMEFWPESLVADLE
                     HKAEDPGQVGLLDWPESIVDGAFDLGPVVYESLATALDPYPKKEGASFQWSQGSGAAE
                     EAESGPFAALQKLKKGQRAGD"
BASE COUNT         1407 a         2603 c         2477 g         1579 t
ORIGIN      
        1 gtgggctccg gcaccggctg aggtgcggcc tgagacgtcg gttcctggcc ctgtttctcg
       61 ttgatcttga gccagctcgg ccgcttggcg ggtttcttcg cctgccacct cgggggcgtg
      121 ctcgcggtct cgtcagtctt cggcgtcgca gacggcgatg cggcaatgac catggggagc
      181 ttccgctcgg gcgcaacgga ggcggctggc gttgccgttg ttgttcgcgc gggtttccgg
      241 cgccgcacct ctggaatgga cgagccggcg gtagcagctt gcctgggctc cggtgcattg
      301 gcggatgctt tggcagcggc agaacgcttc cgtttcgcgc gacgagacgg tttggcttga
      361 ggtgccggca cgtattcttg tgctggcgcc ccctgaataa gctcactggc gagcgtatat
      421 ccaacggcaa gcagcgccgt tgcggccatg acgaggagcg atagcgggac cttcttgggg
      481 aaggacggca gcacggaggc atgcgcgcgg gacacgatgg ccgcttgcgc cggcaccgcg
      541 cccatgtcgt gacgcgcgga cgcatcgcga tagcgcgcta gatatgattc caaaaggtcg
      601 cgattggcct tggcctcgcg ctcaagcgcc ctgagtctaa tctccgcgtc cgactggccg
      661 gccgacttcg acttcgccgc attgaggctc gcacgcaagc tcgcctctct tgccgcggcc
      721 acctgcgcct cattctccag gctcttgact accttgcccg cctcattgcg gatctgggtg
      781 cgcacgtcgg taagttcgga tctcagctgc tgaatacgcg gatgggacgg caagagtgtc
      841 gctgacaact ccgccagttg acgttgcacc tcgacccgct gctcgatcag attgatgatg
      901 agttgcgact tgggcacttc gggcgtggcg ttgatgtcgc cgttatcggc aagcatctgt
      961 ttgatcagct tggcccgggc gcgcgcttca gattcttgcg cctcggacaa aatcagctgg
     1021 ctgttgagct ccgacagttg ttgggcattg agtgtaacgt tgcccgcgcc cgcatagagg
     1081 cccttggagg ccttgaactt ctccaccgct tcttccgatt cggtggttgc cttgcgtaag
     1141 gccttgatct ggtccgcaag ccaggcggtc gcgtccttgg tctgctcgat cttcgccgtg
     1201 cgctgccagc cgatatagac gtcggccaat ttgttggcga tctcggctgc aagcttcgga
     1261 tcgccggagc tgtactccac tgcgatcacc gacgacttcg acagctggaa cacgtcgaga
     1321 ttgtcggcca acgcgttcgc cgcccgctct tgcgccgact ggcgcttgga cccgatgccg
     1381 atcgcgtcca acaggcgcct gagaaatgtc tggcccgcat cgcgctggaa tgttgcgttg
     1441 tggacgaggt cgagatcctt gaccacttgt agaatcaggt ctcgcgaggt gagcacctgc
     1501 acttggctct gcaccgcctg ctcgtccagc ggcgtacgat agccgttccg gccgtcttcg
     1561 ccggtcggcc gcgtaaaggc cgaatcctcg ttctgcacca ggattcgcgc ttcggaggtg
     1621 tagagcggcc gcatcatcga caggccgata aaggtgatcg cgccagctag aagcgttagc
     1681 cccagaatcc aacgctttgc ccgccacagc gcatggccga gcgtggcaag gtcgatgtcg
     1741 gctgccggca aagttttaga cgtgctcact tgaggcgact cctaacgcaa gatggctcat
     1801 taggcgcaga ttgccgtaac caaaagctta agcggcgcat cgcttcggca cgcctgctca
     1861 gcattcgtta accatgcaag agtagcttcg gaccatgttg gagttcgaaa gcgcgccgca
     1921 tggctagatt tgcgaccaga ctcggcctgc tgttgcttgt cagtctcatt gcggggctta
     1981 caggctgcaa gggcggcggc gtgccggggt taacgacgcc ggacttcgcg ctgtccaccc
     2041 ctgcgccact gccggccccg ggcgaggcct atccggcggg cgcaggccgc gcgcctcccg
     2101 gtgtgcctgt gatccccaac gagacctaca cgctcgattc gggcgacagc gtccgcatca
     2161 tcgtcttcgg ccagcagaac ctctccaacg tctatagcgt cgacggctcc ggcgccgtgg
     2221 ccatgccgct gatcgggccg gtccgcgccc gcgggctgac cacgttcgag ctctcctcgt
     2281 agattgccaa aaagctcgcc cgcaaatacg tcaaggatcc gaaggtcacc gtggaggtgg
     2341 ccacctaccg gctcttcttc atcctgggtg aagtcaaccg gccgggccaa tatccttacg
     2401 tcagcgccat gaccgtggag gccgccgccg ccatcgccga gggctatacc gagcgcgcca
     2461 agaagcgcta cgtccgcctc accaggcgct tcggcggcgt catgtccacc gtcatggtcc
     2521 cgaccgatta ccccgttcag cccggcgaca ctatttacgt ccgcgaacgc ttcttctaaa
     2581 gctcatggga aagcctttcc ccctaccggc cctgaagtcc cgtgagttcc aacttccgca
     2641 ttctccattg tctacgtgcg cccgtcggag ggctgttccg tcacgtgcac gatctggccc
     2701 ttggccaagc ggagatgggc gcggaggtcg ggatcgtctg cgattcccgc acgggcggcg
     2761 gcagcgatct cgatgtggcg ctgagccggc tcaaggacaa ctgtgcgctt ggtgtcacgc
     2821 ggattcccat ggcgcgtacc cccgggttca acgattggcg ggtctaccgg aagatcgcca
     2881 agctcgcacg caagctcgac gtggacgttc tgcatggcca cggggccaag gggggcgcct
     2941 atgcgcgcct tgccggccga ggtttacgga agaaaaaggg caccaaggtc gtctacacgc
     3001 cacatggcgg cgtgcttcac tattcgccct ccgcgctgtc gggacggctc tatctcgccc
     3061 ttgagcgcaa gctggggccg atgaccgacg gcctcatttt cgagagcgtg tataccggga
     3121 accgctacgt ggaattagtc gggccgccgg tttgcccggt ccgggtcatc gtcaatggcc
     3181 tgcaccggca cgagttctac gaatccctgc tggcagacga tgcggcggac ttcgtcttcg
     3241 ttggcgagtt gcgcaaactc aaaggcgtcg acgttttcct cgaagcgttg gcggcacatc
     3301 agacgatctt cccgggccgc gccatcatcg tgggctccgg ccctgacgaa aaacacttca
     3361 agcgcatggc gcgtaagctc gggttgacct ccagggtgat gttctcaggc cctcagcccg
     3421 ccagtaccgc gttcgtgcgt gcacgctgcg tcgtcgtgcc cagccgggcg gagtcccttc
     3481 cctatatcgt gctcgaagcg gccgcggcgc agatgccgat gatcgcatcg aatgtcggcg
     3541 gcattccgga gatcgttggc gacgttccca tgccgctgat ccctccaggg gacgtcgatg
     3601 ccctggcggg ccagttgcga gccttcctcg ccgatcccaa tcccttcctc cgccgcgccg
     3661 aggagcttca acgccacgtc gctcagcagt tcacggtcga ggccatgacc cgggacacgg
     3721 tggatttcta tatttccgag ctcggcgcgg gcattcacaa attgcccgag ctctcagcgg
     3781 aaacgacctc ttaaccatag gtcaagcgga gccttccgca cccttccgcc ttcacccatc
     3841 aagaagggct gtcatgcgat gactagaggc tcggagccgt ccggctcagg gtcgcgtcaa
     3901 cgcccggctt tggacgcgga cgaccatcct ctcgatggta atggctgccg tgtgcgccgt
     3961 gggcgtactc gtcgcggccc agagcatccc gaaggtcgcg gagatcgtcg gccagcgtgc
     4021 atccttcgag caatcctacg acgttggccc gcaaggccgc ttcggcggcc aacgcaaggc
     4081 tgtcgaactt gtggccacgc atcccttggg gatcggcgcg cttgagttcg gacgcgtcca
     4141 ccatgtcgag gacgtgcacc aggtctatct cgccatgtat ctcaatgcgg gctggattgg
     4201 tggaacgctc tacctattcc tcgtcctggc gacgatcatg ctcggtctca gactggtgct
     4261 gcgcgaccgg ggcgggaccg gcatcagtgc cgtactcgtc gccgcgttta tcggcatggc
     4321 gctcgaaggc gcggtgatcg acaccgacca ctggcggcac ttctacctgg tcatggcgat
     4381 gatttggggt atggcgctgg tggcgccgcg tgccgtgcgg gacaactggt cctaagcacc
     4441 tagagaagtg gtcggagcgg ggagatttga actcccgacc cccggtcccc cagaccggtg
     4501 cgctaaccgg gctgcgctac gctccgacgt ctcgataggt gagagtgctg catatacgcc
     4561 tttgcgcccc tgagacaagc aaaaggtctc gtggccaaag gggtctctag tctttggtat
     4621 cggcgtccgc caacaaccga tggcaagcct cgagctcctc gaggagcgcg ccgagcgtgt
     4681 cgccgttggg cgcgctgcca ggacgataga ccccaggccc ctgtgccgct tcggcggcga
     4741 tgtcgggaat atagggcgcc gcgatttcgt catccccgga catctcggcg agacgccggt
     4801 agtccatgac gtagcggggg ccctcctcgc gcagaatctt ctggacaccc cggatggtgt
     4861 agccgtcgtt gtagagcagg aaacggatgc cgcgtagaag cgccacgtct tcgggccggt
     4921 agtaacgccg gccaccaccg cgcttcatgg gtttgagctg agcgaacttg gcttcccaaa
     4981 accgcaagac gtgcttgggg acctccagct cgtcagcgac ttcgctaatg gtgcgaaagg
     5041 cctccggcgc cttctccaac ccttcgtcct cctgacgtca agtcccgcgg ctactcggcg
     5101 gccttcgacc gggatagcgc ttcgttgatg cgctgcttca tgatgttgct cgcgcggaag
     5161 acgagcaccc gccggggcgt aattggaact tcctgacccg tcttcgggtt ccgtccaata
     5221 cgctcgcctt tgtcgcgaat gccaaaagat ccgaatgagg acaacttgac cggatcgccg
     5281 cgctcgaggc tcgtcacgat ttcgcgcagc acaagctcca cgatctcggc ggactcgttc
     5341 cgaggcaaac caaccttctg gaagacggcc tccgcaaggt cggcccgtgt tagtgtcttc
     5401 ccgctcatcg tcgtacggct cccccaaagt cgcgggatga accaagatgc tagccgagcg
     5461 cgtgcttgtg ggtcaatctc agacgcaatc aaattcctga ttttgcgtca tattctggcc
     5521 aggtgtgaca ttcccagcac gtagtcacca gcgaatgagc aaggcacccc aggtgaatcc
     5581 gccgcccatg gcctctagga gcaccaaatc gccgctctta atgcgcccat cggtgtaagc
     5641 ctcggttaaa gccagtggaa ttgaggccgc cgaggtattg gcgtgcttat cgactgttat
     5701 gacgacccgc tccggcgcga gcccaagctt ccgcgctgcc ccgtccaaaa tccgcttatt
     5761 ggcttggtgg ggcacgaacc agtcgatgtc ggccgcctca tagcccgcct tgccgagggt
     5821 ctcggtgatg acttggctga tcttggtgac ggcgtgacga aaaacttcgg gcccgtccat
     5881 gcgaagatgg ccgaccgtgc ctgtcgaaga cggaccgccg tctacataga gcttgtccct
     5941 gtagcggccg tctgaccgaa gacacgtcgc caagattccc cgctcggtgt cgacgccatt
     6001 cagcttctgc gcctcgacga cgacggcgcc tgcgccatct ccaaagagca cgcatgttga
     6061 gcggtcctcc caatccaaaa tgcgagagaa ggcctctgca ccgatgacca aggcccgatt
     6121 gaactggccg ctcttcaggt agttgtccgc tgtcgacaga gcaaagacga agcccgaaca
     6181 gaccgcttga acgtcgaatg ctgcgccatg cgtaattccg agttcggcct ggatggtcac
     6241 cgcggacgcc ggaaatgtct gatcgggcgt cgaggtcgcc aggatgatga ggtcgatgtc
     6301 gtcaggttcc agcccggcat cggcgagcgc ggctttcgcg gccgccgcgc ccatggacga
     6361 ggtgagttcg ccatcggcga cgatgtggcg cttgcgaatg ccggtccggg cggtgatcca
     6421 ctgatcggac gtgtcgacga gcgcctccag gtccgcgttg gtgaccacgc gggcgggcaa
     6481 atacccgcca actcccttga tgactgaccg tatggtactc aattcagacg ctcccaagcc
     6541 tacgccgcac cttcaagctc gagcttggtg tggaactggg ctacgtcgct cccgaggcgc
     6601 ttcactacgc cactcgacgc catctcatag gcaaggtcca cggcactggc gaaacccgtg
     6661 gcatcggttc caccgtggct cttgacggcg atgccgttga gcccaaggaa gatgccgcca
     6721 ttcagctggc gcggatccat cttttccttg agtgcgcgaa aggcgccttg ggccaggatc
     6781 gcgccgaggc gagccaggag cgaccgcccc atggcggcac gtaggtactc gccgatttgc
     6841 ttagccgttc cctcggcggt cttcaaggcg atgttgccgg tgaagccgtc ggtgacgaca
     6901 acatccacga cgtcgtgacc gatatcgtca ccctcgacga agccgtgata gcggataggg
     6961 agtggcgcct ctcgcagcac ctgggccgct tccttgatct gctcaacccc cttgacctct
     7021 tcgacgccga tattgaggag accgacagat ggtctctcca tgccgaacag ggctcgggcc
     7081 atcgccgcgc ccatcagggc aaactcgacg atctggcggc tatcggcgcc aacattcgca
     7141 ccgacatcga gcacgatgca ctccgccgca accgtcggcc agatcgccgc gatagcggga
     7201 cggtcgatgc ctttgacggt cttcaaacag aactttgaca tggccatgag cgcgccagta
     7261 ttgccggcgg acacgaccgc atcggcctta ccgtcgcgga cggcctggat ggcgagccac
     7321 atgctggact tccagcggcc gcggcgaaga gcctggctcg gcttggcgtc catggcaatg
     7381 gcgacgtccg tgtggacgac ctccgaccgc tcggccagct gcggataggc ggcgagctcg
     7441 gccacgatct tggcctcgtc gccgaccaag atgaagcgca tgggcggatg acggacgagc
     7501 gcaattgcgg ccgccggaat gaccacgctt ggaccgtggt cgccgcccat ggcgtcgagc
     7561 gctatggtga ctggatcacc cattcctcac accgatctgg aagacgctag gccgcggcgg
     7621 ccgggccgca gcaacccccg agaacgcaac ccgaatagac ttaaaagcgg ggcggtgaca
     7681 acctcatttt gggcttaatc ccccgcccgc tggcctttct tgagcttctg aagggccgcg
     7741 aaaggcccgc tttccgcctc ttccgcggct ccgctgcctt gcgaccattg gaagcttgct
     7801 ccctctttct tgggataagg gtccagagcg gtggccaaac tctcatagac cacgggaccg
     7861 agatcgaagg ccccgtctac gatgctctca ggccaatcta ggaggcccac ttgcccagga
     7921 tcctcggctt tgtgctccaa atcggccacc aaactttccg gccagaactc catctccacg
     7981 ggaatatcca ccgccaccgg aagcggctcc agcgagacca cacaggtctg cgtaaagtcg
     8041 gctgtgagac gtcctgacag gtggat
//
LOCUS       DBNHVU010000182         7070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1997653, whole genome shotgun sequence.
ACCESSION   DBNHVU010000182 DBNHVU010000000
VERSION     DBNHVU010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7070
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1997653"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..2251
                     /locus_tag="AC16Y7_07675"
     CDS             314..2251
                     /locus_tag="AC16Y7_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HIR5709297.1"
                     /translation="MQQSASSETLVANGNGNGNGNGAPALSKRAFWALTFGSIGVVYG
                     DIGTSPLYALRVAVLAAGGEHGIVTQAAVYGVLSLIFWSLFIVVTLKYVLVLLRADNN
                     GEGGTLALTALAFTAVGKRTPLVLALGIVGASMFYGSTLITPALSVLSAVEGLNVATD
                     AFEPYVLPITVIILIALFSVQSHGTAKVSALFAPITTVWFLVIAIAGAYEIAQNPRIL
                     FALNPYYGLNFLFTHGIIGLVTLGAVFLVVTGSEALYNDLGHFGRRPIQTAWLILVLP
                     ALLLNYFGQGALVLLQPETISNPFYLLFPEWALYPMVALAAMATAIASQAVITGAYSI
                     TRQAMQLGLLPRMEVRHTSESQAGQIYMPRVNWLLLVGVLLLVFMFRSSSELASAYVL
                     AVAATEWVAGILGFIVIWLLWRWKFIWVVALMAPLIFVDTAFVMASTLKLAEGAWLPV
                     LVGVMLVVIMVTWRRGTRLLAQKTRRVEVPFAPLIKNLEEKPPHVVPGTAVFLTADPD
                     VAPTALLHNLKHNKVLHEHNVILTIENEDIPRVKIRDRVQIEPVSDRFMRITLRFGFM
                     ERPNVPKALAVASRKGWQFDIMTTSFFLSSRSIRPDARSGMPQWQDRLYVFLAHNADD
                     ASSYFQLPTDRVVQIGTQVTV"
     gene            complement(2261..3190)
                     /gene="miaA"
                     /locus_tag="AC16Y7_07680"
     CDS             complement(2261..3190)
                     /gene="miaA"
                     /locus_tag="AC16Y7_07680"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR5709298.1"
                     /translation="MTASTSRSEIILIAGPTASGKSAAALALAEQLAGTVINADSMQV
                     YRELSMLSARPDAAETAAVPHRLYGTVPANEAYSVGRWLDDAGAAIAEARDAGRMPIL
                     VGGTGLYFKTLLEGLSPVPDIPAEVRAFWRGQANVLSPEDLHQELMARDPVMAARLQP
                     RDPQRVVRALEVIDATDVSLAEWQGREGTPIVARASARKLVVAPEREPLYAVIDARFD
                     RMIEAGAVAEVEALMALGLDPGLPAMRALGVRELSAYLAGRMTREEAAIKAKTESRRY
                     AKRQMTWLKRFMTDWGWFPDSETAVAAASRATP"
     gene            complement(3192..4664)
                     /locus_tag="AC16Y7_07685"
     CDS             complement(3192..4664)
                     /locus_tag="AC16Y7_07685"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HIR5709299.1"
                     /translation="MFQRGNRDQQGPHPAARRWVWSLVGCLVLLAATPGLARGPASVA
                     DIAEGLQDAVVNISTTQTLEGPSDAAPGRKGPKGSPFEEFFDDFFDDEGPDGMPRRVS
                     SLGSGFVIDPSGLIVTNNHVIEGADEIIINFIDGTKLKVTKILGHDPKTDLALLKVEP
                     KKPLTAISFGDSSKLRVGDWVMAIGNPFGLGGSVTVGVVSATKRDINAGPYDDFIQTD
                     AAINRGNSGGPLFNMEGEVIGVNTAIISPTGGSIGIGFAVPSNSATKVLSQLKEYGET
                     RRGWLGVHVQNVTEEIAESLGLNPPRGALVASVTPDGPAAAAGIEPRDVIVDFDGQSI
                     ANMRDLPRAVVGATIGKDVPVQLMRGGETKEVTVKIGRLPEQDEDKEAEAAPMREQDG
                     DDLLGLSIAPLTNDLRNKYGISGNVEGVVITDVTPDSPAAKKKVKPGDVIVEVTQEEV
                     RQPQDVVSRLQAVKRSGRRRVLLLLSDAKGELRFVAVPTS"
     gene            complement(4781..4966)
                     /locus_tag="AC16Y7_07690"
     CDS             complement(4781..4966)
                     /locus_tag="AC16Y7_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HIR5709300.1"
                     /translation="MDELVVAVGLVLVIEGLVWALVPRLGRKLLQVASETPESSLRLA
                     GTVAVAAGVLIVWFVRG"
     gene            complement(4985..5941)
                     /gene="hflC"
                     /locus_tag="AC16Y7_07695"
     CDS             complement(4985..5941)
                     /gene="hflC"
                     /locus_tag="AC16Y7_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0098797 - plasma membrane protein complex
                     [Evidence IEA];
                     GO_process: GO:0052547 - regulation of peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HIR5709301.1"
                     /translation="MKRTALTVLLVLIGLAVVAAYLTLFTVYQTQQALVLEFGKPKRL
                     ITEPGLNYKIPFIQNVQLFDKRLLDLDSAPQEVIAADQKRLVVDAFARWRITNPLLFY
                     QSVNNETGARTQLSAFLEAALRRVLGAASFEAVVRDDRNDLMRKITSELNAKAKSFGI
                     TVVDVRIKRADLPEANSLAIYRRMQTERQREAAEIRAEGEEASRRIRADADRQVTVVK
                     AEATGESERIRGTGDAEKNRVFADAFGKDADFFAFYRSMQAYKGSLRSGDTRMLLSPD
                     SQFFQYFNDAAGGPGGSSSSLPKPTATEDRARSGTSVAPSSP"
     gene            complement(5941..7068)
                     /gene="hflK"
                     /locus_tag="AC16Y7_07700"
     CDS             complement(5941..7068)
                     /gene="hflK"
                     /locus_tag="AC16Y7_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HIR5709302.1"
                     /translation="MPWSNQSGGGGNGGPWGPRPGGPGGGQQPDLEEILRKSQDRLRQ
                     AMPGGGMGGWLAGVLGLLLVLVIGIFAFTVRVNTDEVGIVLRFGEYVRQLQPGLHFRL
                     PYPIEEVELPKFTRINRIEIGMRGSSDRGSTRSVRDVPEESLMLTGDENIVDIDFVVV
                     WKINDAPDYLFNIQNPEGTVKDVSESAMREIVGQSDIEPILTAARAETESKVQQLIQG
                     ILDGYGAGIEIVQVQLQKVDPPAQVIDAFRDVQAARADEERLQNEAQAYANRVVPEAR
                     GAAERILQNAQGYRDRVIAEAKGEADRFEKVLQEYQKAPDVTRKRIFIETMQEVFSNT
                     DKIIIDEKSGGVVPYLPLSELQKRLPPTSAPAPAPQTGGQQ"
BASE COUNT         1227 a         2304 c         2193 g         1346 t
ORIGIN      
        1 aacgctaaag aatggcggaa aacgccggtg taagccgcgc accctaagcg gtgcatgaca
       61 acgggtcaac gagaattgcg aatgaatgac aagcttaacg ctctattgct ttgtgaaaat
      121 tacccgcgct ggggtgcgca tggccattta ttgcgcgctg aggtgcggcc tttggcgcct
      181 gttatctgtg ccaagaaccg tcctagacaa cagcccacga tccgcttatg tccctacggc
      241 tctcaagggg cccgcggcgc ttgggccaag cgcgaacgcg ccaacaacaa cgcccttccc
      301 cacaccggtt tgaatgcagc aatcggctag ctcagaaacg ctcgttgcca acggtaacgg
      361 caatggcaac ggcaacggcg cccccgctct ttccaagcgc gcgttctggg cgctgacctt
      421 cggctcgatc ggtgtcgtct atggcgacat cggcacgagc ccgctctacg cgctgcgtgt
      481 cgcggtgctg gccgccggtg gcgagcacgg catcgtcaca caagcggcgg tgtacggcgt
      541 gctgtcgttg atcttctggt cgctgttcat cgtcgtcacc ctgaaatacg tgctggtgct
      601 gctgcgtgcc gacaataacg gcgaaggcgg cacgctggcg ttgacggcgc tggcctttac
      661 cgccgtcggc aaacgcacgc ctttggttct cgcactgggc attgtcggcg cctcgatgtt
      721 ctatggctcg acgctgatca cgccggccct gtccgtgctc tcggcggtcg aaggtctgaa
      781 cgtggcgacg gacgcgtttg agccttacgt gctgcccatc acggtgatca ttctgattgc
      841 gctgttctcc gtgcaaagcc acggcaccgc gaaggtgtcg gcgttgtttg cgcccatcac
      901 cacagtctgg ttcctcgtca tcgccatcgc cggcgcttac gagatcgcgc agaacccgcg
      961 catcctcttc gcgctcaatc cgtattacgg gctcaacttc cttttcaccc acggcattat
     1021 cggccttgtc acgctcggcg cggtgttcct cgtggtgacc ggctccgagg cgctctacaa
     1081 cgaccttggc catttcgggc ggcggcctat tcagacggcg tggctgatcc tcgtgctgcc
     1141 ggccttgctc ttgaactatt tcggccaagg cgcgctggtc ctgctccagc cggagacgat
     1201 ttccaacccg ttctacctgc tgtttcccga atgggcgctc tatcccatgg tggcgctcgc
     1261 cgcgatggca accgctatcg ccagccaggc cgtgatcacg ggcgcgtact ccatcacaag
     1321 gcaggccatg caactcggcc tcctgccccg catggaggtg cgccacacct cggagtctca
     1381 agccggccag atctatatgc cgcgcgtgaa ctggctgctt ctggtcggcg tgctgctcct
     1441 ggtcttcatg ttccgctcgt cgagcgaact cgcgtcagcc tatgtgctgg ccgtggccgc
     1501 cactgaatgg gtggcgggca ttctcggctt tatcgtgatc tggctactgt ggcgctggaa
     1561 gttcatctgg gtcgtcgcac tgatggcgcc gctcatcttc gtcgatacgg cgttcgtcat
     1621 ggccagcacg ctgaagctgg ccgaaggcgc ttggcttccg gttcttgtcg gcgtcatgtt
     1681 ggtcgtcatc atggtcactt ggcggcgcgg cacgcgccta ctggcgcaga agacgcggcg
     1741 cgtggaagtt cccttcgccc cgctcatcaa gaacctcgaa gagaagccgc cccatgtcgt
     1801 acccggcacg gccgtgttcc tgactgcgga tccggatgtc gcgcctaccg cactgctgca
     1861 caacctgaag cacaacaagg tgcttcacga gcacaacgtg atcttgacca tcgagaacga
     1921 ggacattccg cgcgtgaaga tccgcgaccg ggtgcaaatc gaaccggtga gcgacaggtt
     1981 catgcgcatc acattgcgtt ttggttttat ggagcggccc aacgtcccga aggctctggc
     2041 cgtcgcaagt cgtaaaggct ggcagttcga catcatgacg acgtcgttct tcctctcgag
     2101 ccgctcgatc cggcccgatg cgcgctcagg catgccgcaa tggcaggacc ggctttacgt
     2161 gttcctggcg cacaacgccg acgatgcgtc gagctacttc cagctcccga cggaccgcgt
     2221 ggtccagatc ggcacgcagg tcacggtgta ggcaccaaag ttacggtgta gcgcggctcg
     2281 cggcggccac tgcggtttcg ctgtccggaa accaccccca atccgtcatg aaccgtttca
     2341 gccacgtcat ctgacgcttg gcgtagcgcc gcgactccgt cttggctttt atggcggcct
     2401 cctcccgcgt catgcggcca gccagatagg ccgatagctc ccgcacgcca agggcgcgca
     2461 tggccggcaa gccgggatcc aagccgagcg ccatgagggc ctccacctcc gcgacggcac
     2521 ccgcctcgat catgcggtcg aagcgtgcat cgatgaccgc atagagcggc tcgcgctccg
     2581 gcgcaacgac gagcttccga gccgaagcac gcgcgacaat cggcgtgccc tcgcgacctt
     2641 gccactccgc cagggagaca tctgtcgcgt cgatgacctc gagcgcgcgc acgacgcgct
     2701 gcggatctct gggctgcagc cgcgctgcca taacggggtc gcgggccatg agctcctggt
     2761 ggagatcttc cggtgaaagc acatttgcct ggccgcgcca aaacgcccgc acctcggcgg
     2821 gaatgtcggg cacgggcgac aagccttcga gcagcgtctt gaagtagagc ccggtcccgc
     2881 cgaccaggat cggcatccgc cctgcgtcgc gtgcctccgc aatggcggcg cccgcatcgt
     2941 ccagccaacg gcccacggaa taggcctcgt tggcgggcac ggtgccatag aggcgatggg
     3001 gaacggcggc cgtctcggcc gcatccggcc gcgcgctgag catggaaagc tcgcgataga
     3061 cctgcatgga gtcggcgttg atgaccgtgc ccgcaagctg ctcggcaagc gcgagcgccg
     3121 cagcggactt gccgctcgcg gtgggcccgg caatgaggat tatctcggag cggctcgtcg
     3181 aggcggtcac gctagctcgt cggaacggca acgaagcgca gttcgccctt ggcgtcggac
     3241 aacagcagca ggactcgccg gcgccccgag cgcttgacgg cttgaagccg cgagacgaca
     3301 tcttgcggct ggcgcacttc ttcctgcgtg acctccacga tcacgtcgcc cggcttcacc
     3361 ttctttttag cggcagggct gtccggcgtg acatcggtga tgacgacgcc ctcgacgttt
     3421 ccgctgatgc catatttgtt ccgcaggtcg ttggtaagcg gcgcgatgga gaggccgaga
     3481 aggtcatctc catcctgttc gcgcattggg gccgcctcgg cctccttgtc ctcatcctgc
     3541 tcgggcaagc ggccgatctt gaccgtgacc tccttggtct cgccgccgcg catgagctga
     3601 acaggaacgt ccttgccgat cgtggcgcca accacggcac ggggcagatc gcgcatgttg
     3661 gcgatcgatt gcccgtcgaa atcgacgatc acgtctcgcg gttcgattcc cgccgccgcc
     3721 gcgggcccat caggtgttac tgaagccacc aacgcaccgc gcggggggtt gagccccagg
     3781 ctctcggcga tctcctcggt gacgttctgg acatggacgc caagccagcc ccggcgcgtc
     3841 tcgccatact ccttcagctg gctcaagacc ttcgtcgccg agttggaggg caccgcgaag
     3901 ccgatgccga tcgacccgcc cgtcggcgag ataatggcgg tgttcacacc gatgacctcg
     3961 ccctccatgt tgaacagcgg accgccggaa ttgccgcggt tgatggcggc gtcggtctga
     4021 atgaagtcgt cgtaaggtcc ggcgttgatg tcgcgtttcg tggctgaaac gacgccgacc
     4081 gtgacgctgc cgccgagacc aaacggattg ccgatggcca tgacccaatc gccgacccgc
     4141 aacttggacg agtcgccaaa ggagatggcc gtcaggggct tcttgggttc gaccttgagc
     4201 agcgccaggt cggtcttggg gtcgtggccc aggatcttgg tgaccttgag cttcgtaccg
     4261 tcgatgaagt tgatgatgat ctcgtcggcg ccctcgatga cgtgattgtt agtcacgatc
     4321 aatccggaag ggtcgatgac gaagccggag ccgagcgaac tcacccgccg cggcatgccg
     4381 tcggggccct cgtcgtcgaa gaaatcgtcg aagaactctt cgaaaggcga acccttgggt
     4441 cccttgcggc ccggcgcggc gtccgacggt ccctcgagcg tctgtgtggt ggagatgttc
     4501 accaccgcgt cttgcaggcc ctcggcaatg tcggccactg aggcgggccc gcgcgccaag
     4561 ccaggcgtcg cggcgagcag cacaagacaa ccgaccaggc tccaaaccca gcgccgcgcg
     4621 gccgggtgcg gtccttgctg atcccggttg cctctttgga acatcgaacg agcactccct
     4681 tgccaacgca ggcagccaac ccaaaccgga ccgccaaagc agccgctatc ttaccgaata
     4741 tcggacgatg tgaacgagaa tccgttgctg ccggccacgc ctagccacgg acgaaccaaa
     4801 caatcaggac accggccgca accgcgacgg tgccggcaag gcgaagggac gattcagggg
     4861 tctcagacgc cacctgcagg agcttgcgcc ccagccgcgg gaccaacgcc caaaccaagc
     4921 cttcgatcac cagaacaaga ccgacggcga ctacgagttc gtccatgttc gtcgggccgc
     4981 ttggctaggg cgaggacggg gcgacgctcg tgccgctcct ggccctatct tccgttgccg
     5041 tcggctttgg caggctcgac gaggacccgc cagggccgcc cgccgcgtcg ttgaagtact
     5101 ggaagaactg cgaatcgggg ctgagcagca tcctcgtatc gccggacctc aacgaccctt
     5161 tataggcctg catggagcgg tagaaggcga agaagtcggc atctttgccg aaggcgtcgg
     5221 caaagacgcg gttcttctcc gcatcgcccg taccgcggat tctctccgac tcgcccgtgg
     5281 cctcggcctt caccaccgtg acctggcggt cggcatcggc acggatgcgg cgcgacgcct
     5341 cctcaccttc ggcgcggatt tcggcggcct cacgttgccg ctcggtttgc atgcgccggt
     5401 aaatggcaag gctgttggct tccggcagat ccgcgcgttt gatgcgcaca tcgaccaccg
     5461 tgatgccaaa ggacttggct ttggcgttca actcactcgt gatcttccgc atgagatcgt
     5521 tgcggtcgtc gcgcaccacc gcctcaaagc tggcggcgcc aagcacgcga cgaagcgcgg
     5581 cttcgagaaa ggcgctgagc tgcgtccgcg caccggtctc gttgttcaca ctttgataga
     5641 acagcagcgg gttggtgatg cgccagcggg cgaaggcatc cacgacgagg cgcttctggt
     5701 cggcagcgat cacctcttgc ggcgccgagt cgagatcaag cagacgcttg tcgaagagct
     5761 ggacgttctg aatgaacgga atcttgtagt tgaggccggg ctcggtgatg agccgcttgg
     5821 gcttgccgaa ctcgagcacc agagcctgct gcgtctggta gacggtgaac agcgtcaggt
     5881 aggccgcaac gacggccaat ccgatgagga caagtagaac tgtcagagct gtgcgcttca
     5941 ttactgctgc cctccggtct ggggtgccgg cgcgggcgcc gacgtggggg gcagccgctt
     6001 ctgcaactcc gaaagcggta gatacggaac aacgccgccg ctcttctcgt cgatgatgat
     6061 cttgtccgtg ttggagaaga cctcttgcat ggtctcgatg aagatgcgct tgcgcgtgac
     6121 gtcgggcgcc ttctgatatt cttgcaagac tttttcgaaa cggtccgcct cacccttggc
     6181 ttcggcgatc acccggtcgc gatagccttg cgcattttgc aagatgcgtt cggccgcacc
     6241 gcgggcttcc ggaacaacgc ggttggcgta ggcttgcgcc tcgttctgca agcgctcctc
     6301 gtcggcgcgc gcggcctgca cgtcgcggaa ggcatcaatg acttgcgccg gcgggtcgac
     6361 cttttgcagc tgaacctgga cgatctcgat accggcgccg tagccatcca ggatcccctg
     6421 gatcaactgc tgcaccttgg attcggtctc ggcgcgagcc gcggtcaaaa tcggctcgat
     6481 atcgctttgg ccgacgatct cacgcatggc gctttcggag acgtccttca ccgtgccttc
     6541 cgggttctgg atgttgaaga gatagtccgg tgcgtcgttg atcttccaga caacaacgaa
     6601 atcgatatcg acgatgttct cgtcgccggt cagcatcagg ctctcttcgg gcacgtcgcg
     6661 cacggagcgc gtcgatccgc ggtccgagga cccgcgcatg ccgatctcga tgcggttgat
     6721 ccgcgtgaac ttgggcagtt ccacctcttc gatcgggtac ggcaggcgga aatggaggcc
     6781 aggctgcagc tggcgcacat actcgccaaa gcgcaggacg atgccgacct cgtcggtatt
     6841 gacgcgcacg gtgaaggcaa agatgccgat caccagaacc aagagcagcc cgagcacgcc
     6901 ggcaagccaa ccacccatgc cgccgccggg catcgcttgg cggagccggt cctggctctt
     6961 gcgcaaaatc tcttcaaggt ctggctgctg accgccgcca gggccgccgg gccgcggacc
     7021 ccaaggtccg ccattgccgc cgccgccgct ctgattgctc caaggcatat
//
LOCUS       DBNHVU010000183        24363 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1106426, whole genome shotgun sequence.
ACCESSION   DBNHVU010000183 DBNHVU010000000
VERSION     DBNHVU010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 24363)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 24363)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24363
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1106426"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="AC16Y7_07705"
     CDS             complement(<1..203)
                     /locus_tag="AC16Y7_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019169954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HIR5709303.1"
                     /translation="MTKDCILAALKDVKGPDDLEDDIVSLGLVSEVVIHKGKVYFAIS
                     IDPARAGELEGLRQAAETVVKDLP"
     gene            772..1398
                     /locus_tag="AC16Y7_07710"
     CDS             772..1398
                     /locus_tag="AC16Y7_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709304.1"
                     /translation="MRVVGGSDGSLTSRQADLEGHLDALARFVERHKGLPALECTLVL
                     RSAGSVPAQALTLMKDTLADASVGAKAIVVRLEPQDDLKQIYDTLCALSPETDPAALI
                     RWARDPRLLEAHEQVTYGADMCWAGDAMRRDADKRNPLALFEMDATDAATRAIHAFKA
                     LWSASVPVPGRHLGTPGPVKPSAAYAPMDDAPLPAARKRIQGWPLVRH"
     gene            1489..1896
                     /locus_tag="AC16Y7_07715"
     CDS             1489..1896
                     /locus_tag="AC16Y7_07715"
                     /inference="COORDINATES: protein motif:HMM:NF020395.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family c-type lysozyme inhibitor"
                     /protein_id="HIR5709305.1"
                     /translation="MRVRFLFLLTSLLLAAAPARAQDTGKPMANLLKQPAYVSAWKTM
                     MAGAAAPFWIHEYAKTLDGPPTPVLPVELFGETYLLGFTCKPNACEGNQLFVLFAPRG
                     RDAWGMLAAAPAISWLGRPSQHIKDAITGALRK"
     gene            1965..2972
                     /locus_tag="AC16Y7_07720"
     CDS             1965..2972
                     /locus_tag="AC16Y7_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008505193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR5709306.1"
                     /translation="MQAMLLETVGTPLKASELPVPSPGPHQILLKVLACGVCRTDLHI
                     VDGELDHPKLPLVLGHEIVGRVEAVGSEVTGFTIGERVGVPWLGYTDGTCPYCRMGRE
                     NLCDHPLFTGYQIDGGYAAYTVADERYCFALPEGYSDAEAAPLLCAGLIGYRSLKMAE
                     EAFSGEPGRTKRLGIYGFGAAAHIVAQVARHQGCEIYAFTSPGDEEAQRFARDLGAVW
                     AGGSDEAPPELLDAAIIFAPVGALLPAALRALRKGGIVVCGGIHMSDIPQFPYDILWE
                     ERVVRSVANLTRDDAREFLALAPRVPVKTTVVPMKLSEANEALSALREGRLQGAAVLI
                     P"
     gene            3063..3740
                     /locus_tag="AC16Y7_07725"
     CDS             3063..3740
                     /locus_tag="AC16Y7_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5709307.1"
                     /translation="MTKILIVDDHPMFREALHGAVKSALPDAAVFEAGNIEAACEALR
                     EKSGIDIVLLDLSLPGTAGFEGLMLLRSSFPRIPIMIVSGLDDPRIVHEAIRLGAAGF
                     VPKSVDKTTLANGLSEVLSGSVFVPAELAQALTAPPSLSETDAIADRVAELTPAQMRV
                     LQFLRHGHLNKQIAHELGVSETTVKAHVSEILRKLNVVSRTQAVIATAQLDFETILGS
                     EPTHHRT"
     gene            complement(3737..5113)
                     /locus_tag="AC16Y7_07730"
     CDS             complement(3737..5113)
                     /locus_tag="AC16Y7_07730"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding response regulator"
                     /protein_id="HIR5709308.1"
                     /translation="MSIEWDDLKPGDGPAALERRLEKLIKINAVLMDRVERSMDQQGN
                     AFSLFQTAIGLDAKVRSRTEELTWALRRLERSNEALVLAKDEAERANLSKTRFLAAAS
                     HDLLQPLNAARLTNSVLSEMQESEDAQRLSGQIEQSLQTIEDLIKTLLDISKLDAGVV
                     TPDLQTFPLDTVLSVLEASFGPQAAAKGLKFKVRRCGLLVRSDPLMLQRVLQNLVSNA
                     VRYTKQGGVLVGVRTRDAECVVDVVDTGCGISEAERETMFEEFYRGAAAAEGNHTGLG
                     LGLAIVQRTVAALGYPLEVKSALGKGSTFRLKLEHAGAAPSDSPALWAGSVDNTRGAV
                     VLLIENDEVALDAMRQVLDRWSCQTLSARSLEELTPQLAKLEGPPDIILVDYHLDQGQ
                     TGLAALKSLRETVGPDVPAIVMTGDRGLDTVAEVMTAGCELMHKPIKPAELRALMAHL
                     LGNSASKA"
     gene            complement(5113..6237)
                     /locus_tag="AC16Y7_07735"
     CDS             complement(5113..6237)
                     /locus_tag="AC16Y7_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HIR5709309.1"
                     /translation="MQTAWTEADSADVAVAELASVLDASHIGQLLVFFSRRYEPDVLA
                     GAFAKHFDGVPLVGCSTSGEITPGGYSDGTLLIFAFPKEGFSFVSGVVPDVHNLTVER
                     GTEAVRSLRAKFDRLGKSGGNRFAVSLIDGLSRCEEVTVSAIALALDGIPLVGGSAGD
                     DLVLKGTSLLLDGRVHHDAALLLLVATDYPVHVFNHDHFEPTPTKLVVTASDAESRTV
                     YELNAEPAALEYASSVGIEASSLAPMSFASHPVVVRGGGDYYCRSIQRVNDDGSLSFF
                     CAIDDGVVLTVAQPRDMVASMASELKRIDEEVGGVDLVLGFECVLRRLDAENRQIKHT
                     ISDLYRRYNVAGFHTYGEQYNAMHLNQTFTGVAIGSRERS"
     gene            6680..8200
                     /gene="adh"
                     /locus_tag="AC16Y7_07740"
     CDS             6680..8200
                     /gene="adh"
                     /locus_tag="AC16Y7_07740"
                     /EC_number="1.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HIR5709310.1"
                     /translation="MLHEVIETLNSRVALRPRYDNFIGGDWVAPVDGQYFDNVSPVTG
                     KSVCQVARSQAADIELALDAAHKARDAWGKTAPSERATLLNKVADRMEENLQLLATVE
                     TIDNGKPIRETTAADIPLAIDHFRYFAACVRAQEGTLGEIDHDTIAYHYHEPLGVVGQ
                     IIPWNFPVLMACWKLAPALAAGNCVVMKPAEQTPMSIMVLMDVIGDILPAGVLNIVNG
                     FGVEAGKPLAQNKRIAKIAFTGETTTGRLIMQYASENLIPVTLELGGKSPNIFFDDVM
                     AEDDDFFDKALEGFAMFALNQGEVCTCPSRALVQKSIYDRFIEKAVERVNKVKQGNPL
                     DADTMIGAQASNDQLEKILSYIDIGQKEGAKLLAGGERNVLPGDLAEGYYVKPTVFEG
                     HNKMRIFQEEIFGPVLSVTTFEDEEEALEIANDTLYGLGSGVWTRDINRAYRFGRGIQ
                     AGRVWTNCYHLYPAHAAFGGYKKSGIGRETHKMMLDHYQQTKNVLVSYSPKALGFF"
     gene            8330..8680
                     /locus_tag="AC16Y7_07745"
                     /pseudo
     CDS             8330..8680
                     /locus_tag="AC16Y7_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
     gene            8711..9100
                     /locus_tag="AC16Y7_07750"
     CDS             8711..9100
                     /locus_tag="AC16Y7_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5709311.1"
                     /translation="MPLKGRRVVIADDDRIIREFLSLRCAQLGLTVETAEDGAGAVDT
                     IAGEDTDLLIVDLNLPDMNGFDIVERLAKSEPRPPVIVLTGSTDEDLKRRCRDLNVPF
                     VHKNADTWNVLEPLIREIVGARSASNA"
     gene            complement(9097..10848)
                     /locus_tag="AC16Y7_07755"
     CDS             complement(9097..10848)
                     /locus_tag="AC16Y7_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR5709312.1"
                     /translation="MLHVPPLVSTLTIGLVLAFSLGVVAHRLKLPPLVGYLVAGIAIG
                     FSPSVGADQNIANQLSEVGVILLLFGVGLHFSVKDLLSVRAIAIPGAVLQGLVATPLG
                     MGVGWMLGWSLGAGFIFGLALSVASTVVLLRMLQERRLIDTERGRITVGWLIVQDIAM
                     VMALVLLPAITGLFEGDAPPDLTEMARQCAEVIGKLAIFTFVMFVVGKKLIPMLLHYV
                     AHTGSRELFRLAVLSIALGFAFLAAELFGVSLALGAFFAGMVLSESQLSQQAATESLP
                     LRDAFAVLFFVSVGMLFHPAIMMDEPLAVLATVAIIVVAKSIVAYSIVRAFGYSHDAG
                     LMISASLAQIGEFSFILASLGQSLGVLPQEGRDLILAGAIISILLNPLLFAALDGYFA
                     RRERLAAEGVVPEGTDMDAETEEEEATPTREPIVPTTLTDHVVLVGHGRVGKFISKQL
                     RERGTPLYIVEDDADDVADLKSQGIEALVGNAADPEVVQSANLGDARCLLVAIPDAFE
                     GGQVVQQARDINPVLPIIARAHSEEEINHLVKLGANLVVMGEHEIAKAMIGDVEGVRS
                     PTRGDDGAPRPRTSSFA"
     gene            11196..12476
                     /locus_tag="AC16Y7_07760"
     CDS             11196..12476
                     /locus_tag="AC16Y7_07760"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR5709313.1"
                     /translation="MTPSFDMFGLAEPLRRALNAENYQTPTPIQVQAIPILTEGKDLL
                     GVAETGSGKTAAFALPLLHNLSGDTSPRKPGMPRALILAPTRELAGQIADRINVYGRH
                     LKLRTAVIFGGVGQGQQVKALRAGVDIVVATPGRLLDLCNQRHIRLSEVQTLILDEAD
                     RMLDMGFIHDVRKIVGFCPKERQTLLFSATMPPSIAKLAHDILRNPVRVDISRAKVAV
                     DRIAQRVYFVGARDKRTLLTQLVTDPALSQAVVFTRTKRGADRVCRHLVEAGLTAAAL
                     HGNKAQNARVRALDGFRSGRVQILVATDIAARGIDVPGISHVINYELPNEPESYVHRI
                     GRTARAGAQGTALSLCDNSERPYLKAIERLMRVDVSVVEHTLAEREDTSPRPGNRNGA
                     KRHAAKKRRTNRGRRPNRAAGKSRGANQQQEVRL"
     gene            12473..12727
                     /locus_tag="AC16Y7_07765"
     CDS             12473..12727
                     /locus_tag="AC16Y7_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709314.1"
                     /translation="MNKTISRMSAARRSAEAILSQSQKRQESFKREQQQASDALAEKT
                     ARLRELRLAKEAADREAEAAVAAAAPKPKKRAAVRRSKQT"
     gene            12749..12931
                     /locus_tag="AC16Y7_07770"
     CDS             12749..12931
                     /locus_tag="AC16Y7_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709315.1"
                     /translation="MAFKPNYNFQKAERARAKAQKKQEKLRRLEEETAKRKALRDGVP
                     ETGTPDEDAGTPTDDA"
     gene            12948..13196
                     /locus_tag="AC16Y7_07775"
     CDS             12948..13196
                     /locus_tag="AC16Y7_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709316.1"
                     /translation="MARKQQARPSGFVLFDVIYEDGRQTSNRKVPATELGGLDGDAPA
                     RTIIEAQDQTIAEMSGKPRSPIKSLARSRKAKAKAKAS"
     gene            13304..13921
                     /locus_tag="AC16Y7_07780"
     CDS             13304..13921
                     /locus_tag="AC16Y7_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709317.1"
                     /translation="MQILVAAVKGVLAFSVAMLFVDDARAQVPIEEDAGTITMTGTVE
                     EIKVPERVLMIVGPGGRTIVGVISPDVKDIKTIKLKEKVTIQFTQEVAVALRKADGVP
                     EAVKNKFAESETADMGMNAPTVAEQSWTNLTPSGGTANLTTIELTATIAAVNKNQRTV
                     SFAGTGGMTRTVYVPPSIVGFDQIQVGDRVVIEITRTSIVNVKAL"
     gene            complement(14008..14448)
                     /locus_tag="AC16Y7_07785"
     CDS             complement(14008..14448)
                     /locus_tag="AC16Y7_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709318.1"
                     /translation="MIRPVIAALALGALMCGPAVAEDASDRPTRMGEDKEKTVFVQLI
                     VKACRGVTVPLEPTNHGKDYNNEKPVTRAESMAAAIATGCIDVPVPMEFLAEGMSPNA
                     CLGHGGYLAAMQFLRQRQDLREFPAVAAWNCVITDHEVVGVSTM"
     gene            complement(14651..15259)
                     /locus_tag="AC16Y7_07790"
     CDS             complement(14651..15259)
                     /locus_tag="AC16Y7_07790"
                     /EC_number="1.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF016180.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:1901053 - obsolete sarcosine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma"
                     /protein_id="HIR5709319.1"
                     /translation="MSKLTSRSAFEGILTPGRIGAKDGEAGVTVTERVGLGLASLEVR
                     EGQTDALKSCVRDAYGAALPEGSTVAFGKDVMFIGTGPGQWLAVSETIGNEDLAAHLA
                     AKLEGCASVADQSSGRGALRFSGPRARDCLAKGLAIDLDLRVFPAGSAATSTLSHMGV
                     ALWREGEAESFNIVLNRSMADSFWRWLKASAAEYGCQAVTAS"
     gene            15515..16246
                     /locus_tag="AC16Y7_07795"
     CDS             15515..16246
                     /locus_tag="AC16Y7_07795"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR5709320.1"
                     /translation="MSDQPTRDEETMLARLSKPTSGVGTITAYLQRAIETGAYSEGDK
                     LPPERQLAETFKAARSTVRRALDRLERDGLVSRRLGSGTFVGASAVAGQRPAGLVDEL
                     SPLQLIEARLAVEPFTARLAVLHATRRHLDDMDAILAKAEAAAHDKDEFSKWDGELHL
                     LIARASANPLLISVYRQINHARLHAQWDAMKEKILTPDVIDEYNRQHGGILRAIHVRD
                     AQLAQALMAEHLEKARDDLLRAANP"
     gene            16518..17360
                     /locus_tag="AC16Y7_07800"
     CDS             16518..17360
                     /locus_tag="AC16Y7_07800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5709321.1"
                     /translation="MRPAEGLTKRAHRLVLTGLCVTAATCLGVPALAQTVPPVETEVV
                     REAPVVRKLELSGTVTSPHASQISTSVGGLVSKVYFDSGAHVKTGDLMLELDAELEEA
                     AYEQAEAQAAQAAGEVKDAKRRLRIAESLAKRKYGPQNEVEARQAEVDIDTATHESFK
                     AEQKRRQVVLQRHQLPAPYDGVISKRMAEVGQWVTPGTAVFELVAMHDFRVDVPVPQD
                     YYARLRQGADVRIEIDALPGELVPATIGALIPVSDPDARTFTLRVLPNATTSPSRPAC
                     RPAS"
     gene            17342..17614
                     /locus_tag="AC16Y7_07805"
     CDS             17342..17614
                     /locus_tag="AC16Y7_07805"
                     /inference="COORDINATES: protein motif:HMM:NF052838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permuted SH3 domain-containing protein"
                     /protein_id="HIR5709322.1"
                     /translation="MSARVLIDLDTGKQDVAVNRDALIRYPDGRITVWVVKQDGDTVS
                     VTERRVEIGLAFDGRVQVLKGLKEGDRVVVRGNESLREGQTVKLAS"
     gene            17635..20814
                     /locus_tag="AC16Y7_07810"
     CDS             17635..20814
                     /locus_tag="AC16Y7_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5709323.1"
                     /translation="MLEQIVKRGTLVTVIALIVCVLGIVAAFRIPVQMIPDLEVRTVT
                     VHTRWAGATPQDVEKEIVIEQEEYLRSIPSLERITSRASSGQARIEMEFPYNIDLNET
                     LIRINNALSQVPSYPEDVDQPRVRASSFSANSFMYFRVSPLPGNPRQLNMDMMRDFID
                     DNVRARLESVTDVSDVGFGGGAEKQIQILFDPAALAERKISLTDVRRAVAARNRDTSG
                     GEIESGKRRYLLRTIGRFDNPEDLKELIIARRGDNIIRLEDVADVRLGHFEIYTRAYV
                     NDQPIIFLSIRREAGSNVIAIKEAVMKEVEEINREVLIPTGMELELTADDVNYVEASI
                     RNVWTNLSIGAVLATLVMWLFLRSAKATVVGVIGIPICTIAAFIGLMVAGRTVNVISL
                     AGVAFAIGMTLDNTIVVLESIDLERRKGLDRLKAAIEGVRKVWPAVLASTLTTVLVFV
                     PIVFIQEEAGQLYSDIAIAVSASILASMIVAITVVPTAAAHFDLTPQDNKAVDRADGA
                     IVKGVGWLVGGPIRRLLCILGAAGASAAIILLLTPPAEYLPEGEEPKMFARLSAPPGY
                     NLDTMTEIGTDLQDWLLPFVKDDPARFHRGETEVPAIKYFFLMMEADSLRIIAETIDP
                     GDIGKLMKVVGERYRTYPGMRPFVSRGSIITSNDGGTRSVSLDISGPRLQDIYNVAST
                     AYRRAGEIFDTPRIQAEPSTLTLSQPLIEVRPKWDRAAELGMTSDDVGFTVAALTDGA
                     YVGEFFRDDEKIDIYFYSKDALRTSLDTLPDMPVYTPQGTIVPLSSVADIKETVDTNS
                     IRRIDGKRTVTLNIIPPDDVALETGVEIVKSQLVDHMKRAGDVPFDVSMTISGASDQL
                     DATREALAGNYIVAVVIIYLLLVAIFTHWGYPLLIMTAIPLGVAGGLVGLALMNLVGA
                     ILPQFGVAPIIQPFDMITMLGFLILMGTVVNNPILIVDRALYNLREMGLDAKDAVMEA
                     VSSRLRPIAMSTVTTICGLAPLVFIPGAGTELYRGVGAIVLFGILSTAIITLTFLPAL
                     SITVFRLAGYGAKANAKRGRIEASPAPGE"
     gene            complement(20917..21099)
                     /locus_tag="AC16Y7_07815"
     CDS             complement(20917..21099)
                     /locus_tag="AC16Y7_07815"
                     /inference="COORDINATES: protein motif:HMM:NF021621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HIR5709324.1"
                     /translation="MFRKRHNSASKGRPEFVLVAPPKVLGEVRQALPATLAKVLTDTF
                     TKDLTKIPDHSFQRNC"
     gene            complement(21056..21523)
                     /locus_tag="AC16Y7_07820"
     CDS             complement(21056..21523)
                     /locus_tag="AC16Y7_07820"
                     /inference="COORDINATES: protein motif:HMM:NF021536.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HIR5709325.1"
                     /translation="MTTGLDTFDTTVQESNRWLKAVMDRLNTTDRHRAYSCLRAVLHA
                     LRDRIGPESAAHLGAQLPMLLRGLYYEGWDPTNKPTKERHKEAFLAHIAQELPRATED
                     EVEQGALAVLDVLSKYVDRGAAIKIAVMFPRDLRKFWPTFVQEAAQQRQQRAS"
     gene            complement(21771..22148)
                     /locus_tag="AC16Y7_07825"
     CDS             complement(21771..22148)
                     /locus_tag="AC16Y7_07825"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR5709326.1"
                     /translation="MLSTEPFWRGEFGLAQAPAVDIVEHDKSYQVTAELPGMEETDID
                     VKFADGVLTIKGEKKEESEEKQKDYYRSERRFGAFQRSFRVPDGIEPDKIEATVKNGV
                     LTVLLPKSDDAQKREKKIEIKKG"
     gene            complement(22494..23783)
                     /locus_tag="AC16Y7_07830"
     CDS             complement(22494..23783)
                     /locus_tag="AC16Y7_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709327.1"
                     /translation="MGDLQPSSDAETIMRQVSEICRSQRFHDAPVLQRILRYTVESTL
                     AGESAQLGATTVGRFALGKGLKFRSSKDSSVRVAFNRLRNALALYYSAEGQKDDIVIR
                     FAPGCYVPSFSHARSDEDNGPATALRWADWYQGIATMAAHQRASAAVQVELTSHPDHP
                     DLLAAYADLLLDGHKHGYDDDPRAVQYVVDLIDRAKDARPDNARVQFVDSFLALHLGN
                     KDAVAQIGRALTEPHNSRINRAQGTWMLALVSDPSDFGDRPEWDLFDQTGLPGWIHHA
                     RFVVSYANGEYEDALHAAMRFGMRDWYWGYIDRAAALAQLGLKTAASLELQQLVLLNH
                     QFPKNPERFLSSYVPNCSLVEHIKEGLEKAGLSKLDAHGRPPGKSKPGWSALFSTRQQ
                     SSHVLETRTRFAECGIDVRFWHFTDIDFDARDFCFRG"
     gene            23921..>24363
                     /locus_tag="AC16Y7_07835"
     CDS             23921..>24363
                     /locus_tag="AC16Y7_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709328.1"
                     /translation="MLRTAFVALILLITTSAGVAAETADSAYINGKIYTVDATKPWAE
                     AVAIKDGKFIAVGSNEAIAAVTGEGTKVVDLDGGFAMPGIHDTHVHPPLVYGHEEAGR
                     LLFSETNSPDEIIEIVKEYAAKNPDIEIIRGEKWAAGDFPGGKAT"
BASE COUNT         4834 a         7641 c         7480 g         4408 t
ORIGIN      
        1 ggcaggtcct tcaccaccgt ctcggccgcc tggcgcaagc cctccagctc gcccgcgcgg
       61 gctggatcga tggaaatggc gaagtagacc ttgcccttat ggatcaccac ctcggaaacg
      121 aggccgaggg agacgatatc gtcctcaaga tcatccgggc ctttcacgtc tttcagcgct
      181 gcgagaatgc agtctttagt caatgtctct gtcatcatgc cagggaatgt agtgaggaag
      241 ctgttgcaac gcaaaatggc caatgaatgg gcccattgga gcggccccga caacggcatc
      301 aaaggacgct tcacctggct gttctgatac ttgagcacgg caagaaaaag cccggcgcga
      361 gggccgggct ttggagagaa tcgggagcca gcgcgggcgg ggtttggggg tagggtgcgc
      421 accgcgccgg acccgaaagc acaacgcgaa gcacccgaaa cggttccata tcgaggtccc
      481 aggcccgtca cattttgccc gcttcaatga ggatggatgg gccgctagaa gcgtcggcgc
      541 atccgtttta tgcggcgaat tttgcccctt tatcattggc ttatccccaa gattggacgg
      601 actgccccga tgccgtattg cggccgatac cctttggcgt tccaaaaaat acgctgcgaa
      661 agccttcttt aacctgctcc gggccattct cggtctggac agggcggagc tccggtacat
      721 ttcgtaagat ttctcgccca gcagccactt ccacgaagcc tagggggcaa aatgcgcgtt
      781 gtgggcggtt cggatgggtc tctgacgtct cgccaggcgg atctcgaagg gcatcttgat
      841 gccctggcgc gtttcgtcga gcgccacaag ggtctcccgg ctcttgagtg cacgctggtc
      901 ctgcgctcgg ccggttcggt tccggcacag gcgctgacgc tgatgaaaga cacgctggcg
      961 gatgcgagcg taggagccaa ggcgatcgtc gtcagactgg agccccagga cgacctcaag
     1021 cagatttacg acacactctg cgccttgagc ccggagacgg accctgcggc ccttatccgc
     1081 tgggcgcgcg atccgcgcct gctcgaggcc catgagcaag tcacctacgg cgccgatatg
     1141 tgctgggcgg gcgatgccat gcgccgggat gccgacaagc gcaatccgct tgccctgttc
     1201 gagatggacg caaccgacgc cgcgacccgc gcgatccatg ccttcaaggc tttgtggagc
     1261 gcgtcagttc ccgtgccggg gcgtcacctc ggtacaccgg gcccggtcaa gccgtcagcc
     1321 gcctatgccc caatggacga tgcgccgttg ccggctgcgc gcaagcgcat tcaggggtgg
     1381 cctctcgtcc gccactaggc gtcacctccc gtgttttgta aaactcccgt cacaatcatc
     1441 tcccagaggt gcaaggattc gcacctcaca gctgatgacg gagtcggtat gcgcgtcaga
     1501 tttctattcc ttctcacatc tttgctcttg gccgccgcgc ccgcgcgtgc gcaagacacg
     1561 ggcaaaccga tggcaaatct tctgaagcag ccggcctatg tctctgcctg gaagaccatg
     1621 atggcgggcg cggcagcgcc gttttggatc cacgaatacg ccaagaccct ggatggtccg
     1681 ccaacgccgg tcctccccgt ggagctcttc ggcgagacct acctgttggg ctttacctgc
     1741 aaacccaatg cctgcgaggg caaccagctc tttgtgctgt ttgcgccgcg cgggcgggac
     1801 gcctggggca tgctggccgc cgcgccagcg atctcgtggc tgggcaggcc gagccagcac
     1861 atcaaagacg cgattacggg cgctctcagg aagtagatcc cgtcgaacgc ggctgccgcg
     1921 gcgcctccgt gtgctagacc agaggtccaa tggtgcaaga gaccatgcag gccatgctgc
     1981 ttgagacggt cggtacgccg ctcaaggcca gcgagctgcc ggtgccgtcg ccggggccgc
     2041 accagatcct cctgaaagtg ctcgcctgcg gcgtctgccg caccgacttg cacatcgtcg
     2101 acggcgagct cgaccacccg aagctgcctc tcgtgctcgg tcatgagatc gtcgggcgtg
     2161 tcgaggccgt gggctcagag gtgacgggct tcaccatcgg ggagcgggtc ggcgtgccat
     2221 ggctcggtta cacggacggc acctgcccct attgccggat gggccgggag aatctctgcg
     2281 accacccgct attcaccggg taccagatcg acgggggcta cgcggcctac accgtggcgg
     2341 acgagcgcta ctgtttcgcg ttgccggagg gctattcgga tgccgaagcc gcgccgctgc
     2401 tctgcgccgg gctgatcggc taccgctcgc tgaaaatggc ggaagaggcc ttctcgggcg
     2461 agccggggcg aaccaagcgg ctcggcattt acggcttcgg cgcggcggcg catatcgtgg
     2521 cgcaggtggc gcgccatcaa ggctgcgaaa tctacgcctt caccagcccg ggcgacgaag
     2581 aggcgcaacg cttcgcgcgc gacctcggcg cggtgtgggc aggcggttcg gacgaggcac
     2641 cgcctgaact gctcgatgcg gcgattattt tcgcacccgt cggcgcgctt cttccggcgg
     2701 cgttgcgggc actacgcaag ggcggcatcg tggtgtgcgg gggcatccat atgagcgaca
     2761 tcccgcaatt cccctacgac attctatggg aggagcgggt ggtccgctcg gtggccaatc
     2821 tcacccgcga cgacgcgcgg gagtttctgg cgctggcgcc ccgggtcccc gtgaagacga
     2881 cggtcgtgcc catgaagctg tccgaggcga acgaggcgct gtccgcctta cgcgagggca
     2941 gacttcaagg cgccgcagtt ctcattccct agctgcggac ttcctactac tataagtgta
     3001 ttgcgcctga gaggattttg cggttttccg cgggggaaac gccccaacac tgggccccag
     3061 gcatgaccaa gatactcatc gtcgacgacc accccatgtt ccgcgaggcg ctgcacggcg
     3121 cggtcaagtc cgcattgccg gatgcggccg tctttgaagc cggcaacatc gaggcggctt
     3181 gcgaggccct gcgcgagaaa tcgggcatcg atattgttct gctcgacctt tcgctccccg
     3241 ggaccgccgg gttcgaagga ttgatgctgt tgcggtcgtc cttcccgcgc attccgatca
     3301 tgatcgtctc aggcctagac gatcccagga tcgtgcacga ggcgatccgg ctgggcgcgg
     3361 ccggcttcgt acccaaatcc gtcgataaga cgacgctggc caatgggttg tcggaggtgc
     3421 tgagcggctc ggtgttcgtg ccggccgagt tggcgcaagc cctgacggcc ccacccagcc
     3481 tgtcggagac ggacgcaatc gccgaccgcg tcgccgagtt gacgccggca caaatgcgcg
     3541 tcctgcaatt tctgcgtcat ggccatctga acaaacagat cgctcatgaa ctcggcgtga
     3601 gcgagacgac cgtgaaggcg catgtgtcgg agattctacg gaagctcaat gtcgtgagcc
     3661 gtacgcaggc cgtgattgcg acggcgcaac tcgacttcga gaccatcctc ggcagtgagc
     3721 ccacgcacca caggacctaa gccttcgagg cggaatttcc caacagatgc gccatcagcg
     3781 cacgcaactc ggcgggcttg atcggcttgt gcatcaattc gcagcctgct gtcatgacct
     3841 cggcgacagt atcgaggccg cgatcgcccg tcatgacgat ggcgggaacg tcgggcccga
     3901 cggtttcgcg caaggacttg agggcggcaa gccccgtctg gccttggtcg agatggtagt
     3961 cgaccaagat aatgtcgggg gggccttcca acttcgcgag ctgcggagtc agctcctcca
     4021 agctgcgcgc cgatagagtt tggcaggacc accggtccag cacctgccgc atggcgtcga
     4081 gcgcgacctc gtcgttctcg atcaacagca cgacggcgcc gcgcgtattg tcgacgctgc
     4141 cggcccataa ggccggacta tcgctggggg cggcacccgc atgctcaagc ttgagccgga
     4201 aggtcgagcc cttgccgagc gccgacttga cctcgagcgg gtagccgagc gccgcgaccg
     4261 tccgttgcac gatggcgagc ccgagcccga ggcccgtatg gttgccctcg gccgccgccg
     4321 caccgcgata gaactcctcg aacatcgttt cgcgctcggc ttcggagata ccgcagcccg
     4381 tgtccacgac atcgaccacg cactcagcat cgcgcgttcg cacgccaacc aggacgccac
     4441 cctgtttggt atagcgcacg gcgttggaca cgagattctg caacacgcgc tgcagcatga
     4501 gcggatcgct gcgcactaag agaccgcaac gccggacttt gaatttcaga cctttggccg
     4561 cggcctgcgg accgaagctc gcctcaagga ccgataacac cgtgtccaag gggaaggtct
     4621 gcaagtcggg cgtcacgaca cccgcatcga gcttggagat atcgagcagc gtcttgatca
     4681 gatcttcgat cgtctgaagc gactgttcga tctgcccgga aagacgctgc gcatcctcgc
     4741 tttcttgcat ctcggacagc acggaattcg tcaggcgcgc ggcgttgagc ggctgcaaaa
     4801 ggtcgtggct ggcggccgcc aggaagcgcg ttttactcag attggcgcgc tccgcctcgt
     4861 ccttggccaa gaccagggcc tcgttcgagc gctccaggcg ccgcagcgcc caggtcagct
     4921 cctcggtgcg cgaacgcact ttggcatcga gaccgatcgc cgtttgaaac agcgagaagg
     4981 cattgccctg ctgatccatc gagcgctcga cgcggtccat cagaaccgcg ttgatcttga
     5041 taagcttctc caagcgccgc tcgagcgccg caggtccgtc accgggcttg aggtcatccc
     5101 actcgatcga catcaactgc gctcccggct gccgatcgcg acccctgtga aggtctgatt
     5161 gagatgcatg gcgttgtact gttcgccgta agtgtggaag ccggcaacgt tgtagcggcg
     5221 gtatagatcg ctaatggtgt gcttaatctg ccggttctcg gcatcgagcc ggcgcagcac
     5281 gcattcgaag ccgagcacga gatcgacacc gccgacttct tcgtcgatcc gtttcagctc
     5341 cgaagccatt gaggcaacca tgtcgcgcgg ttgcgccact gtcagcacga caccatcgtc
     5401 gatggcgcag aagaacgaca gcgagccatc gtcgttcacg cgctgaatgg atctgcagta
     5461 atagtctcca ccaccgcgca cgacgaccgg atgcgaagcg aaactcatgg gcgcaagcga
     5521 cgaggcttcg atgccgaccg agcttgcata ttcgagagcc gccggctcgg cattgagctc
     5581 atacaccgtc cggctctccg catcggatgc cgtcaccacc agcttggtgg gggtgggttc
     5641 gaagtgatcg tggttgaaga catgcacagg gtaatcggtc gcgaccagca gcagaagcgc
     5701 tgcatcgtga tggacgcgtc catccaaaag caagcttgtg ccctttagga cgagatcgtc
     5761 gccggcggac ccaccaacga gcggaatgcc gtcgagcgcc aaggcaatgg ccgagacggt
     5821 cacctcctcg cacctggaga gtccgtcgat gagagaaacc gcaaaccgat tgccgccgct
     5881 tttgccaagt cgatcgaact tcgcgcgcaa cgaccggacc gcttcggtcc cccgctcgac
     5941 ggtgaggttg tgcacgtcgg gaacgacccc gctgacgaaa ctgaagcctt ctttcggaaa
     6001 ggcgaagatc agcaaggtgc cgtcgctata gccgccgggt gtgatctcgc ctgacgtgct
     6061 gcaaccgaca agcggaacgc catcgaagtg tttcgcgaag gcgccggcca acacgtccgg
     6121 ctcgtatcgc cgggagaaga aaacgagaag ttgtccgatg tgcgaagcgt cgaggacgct
     6181 ggccagctcg gccaccgcga cgtcggcgct atctgcctcc gtccaggccg tctgcacgcc
     6241 gcacgcatga cgcttcgctt gaggcccgcc ttgcaaacgc ttcctccacg cgatgccgcc
     6301 ccaattcttg attttcagcg caatccggcg atttcttccg ccagcggttg ggggggcact
     6361 gccaacatgt tggtcaaagg tagaatacca cccggggaat cccgccgata gtctccttcg
     6421 ccgagcccac aatccctctt ccgtgggccg ttcgttgaag agttccgctc tctagagttc
     6481 caaccaatcg aacaccattg ccttaccaca cctcaccgcc ccccatttgg gggtggttct
     6541 ttatcgcgcc cgggcccatt gggtccgctt acgcccaaag tacaattttc atggtcagca
     6601 ggctgccttt agtctgcgag gctagattag aagacgccca gaaaacgcga ccagtaagct
     6661 ggttggttgg gaggtttcca tgttgcatga agtcatcgag accctgaatt cgcgcgtggc
     6721 tttgcggccg cgctacgaca acttcatcgg tggcgattgg gtcgcccccg tcgacggtca
     6781 atatttcgac aatgtcagcc cagtgacggg caagtccgtg tgccaggtgg cgcgctcgca
     6841 ggccgccgat atcgagcttg cactcgacgc ggcgcataag gcgcgcgacg cctggggcaa
     6901 gacggcgccg tcagagcgcg cgacgctgct caacaaggtc gccgaccgca tggaggagaa
     6961 cctccaactg ctcgcgaccg ttgaaaccat cgacaacggc aagcccatcc gcgagacgac
     7021 cgcggcggat atccccctcg ccatcgatca cttccgttat ttcgcggcct gcgtccgcgc
     7081 gcaggaagga accctcgggg agatcgacca cgacaccatt gcctaccact accacgagcc
     7141 gcttggcgtg gtgggccaga tcatcccgtg gaatttcccc gtcctcatgg cctgctggaa
     7201 gcttgcgccg gcacttgccg ccggaaactg cgtggtgatg aagcccgccg agcagacgcc
     7261 catgtcgatc atggttctca tggacgtcat cggcgacatc ctgcccgctg gcgtgctcaa
     7321 catcgtcaac ggtttcggcg tcgaagccgg caagccgctc gctcagaaca agcgcatcgc
     7381 caagatcgcc ttcaccggcg aaaccacgac cggccgcctc atcatgcagt atgcgtcgga
     7441 gaacctcatt cctgtgacgc tggagcttgg tggcaagtct cccaacatct tcttcgacga
     7501 cgtgatggcc gaggacgacg atttcttcga caaggcgctg gaaggcttcg ccatgttcgc
     7561 gttgaaccag ggcgaggtgt gcacctgccc gtcgcgggcg ctggtgcaga agtcgattta
     7621 cgaccgcttc atcgagaagg ccgtcgaacg ggtgaacaaa gtcaagcagg gcaacccgct
     7681 cgacgcggac accatgatcg gcgcacaagc ctccaacgat cagctggaga agatcctctc
     7741 ctatatcgac atcggacaga aggaaggcgc caagctgctc gccggcggtg agcgcaacgt
     7801 actccccggc gatctcgccg agggctacta cgtcaagccc acggtgttcg aaggccacaa
     7861 caagatgcgc atcttccagg aggagatctt cggcccggtc ctgtcggtga ccacgttcga
     7921 ggacgaggaa gaggcgcttg agatcgccaa cgacacgcta tacggcctcg gctcgggggt
     7981 gtggacccgc gatatcaacc gcgcctaccg gttcggccgg gggatccagg ccggacgggt
     8041 gtggacgaat tgctatcacc tctacccggc ccacgcggcc tttggcggct ataagaaatc
     8101 gggcatcggg cgcgaaaccc acaagatgat gctcgatcac taccagcaga ccaagaatgt
     8161 gctggtgagc tatagcccca aagcactggg attcttctaa gaactggcac ccaagggatt
     8221 gcacaagacg gcgcctggca ggacttgtcc cgccgggccc gcctcacaat attcacccgt
     8281 ggcaatcgcc taggcgtgcg gaagcgcctc gacgagggag acgggagcga tggtagacag
     8341 ggtgctggtg acaaacgccg ccctcgagtt ggtggagaag ctcaggtcgc tccatggccc
     8401 gctgatgttc catcagtcgg gcggctgttg cgacggctcc gcgcccatgt gctatccggc
     8461 cggcgagttc acagttggaa gcaacgacgt gcacctcggc gagatcggcg gctgtcctgt
     8521 ctacatggac gccgaccagt tcgaatattg gaagcacacc caactgacga tcgacgtggt
     8581 agaggggcgc ggctcaggtt tctcgctgga agcgccggag ggtgtgcgct ttctcacgcg
     8641 aagccgcgtc ttcaccgacg aggaagccga tgcgttggcg cgcgccgagg acgccgccct
     8701 gcaatcaaat atgccgctca agggccggcg tgtcgttatt gccgacgacg atcgcatcat
     8761 tcgtgagttt ctatcgctcc gttgcgccca gttgggcctg acggtcgaga ccgccgaaga
     8821 cggggcgggc gccgtcgata cgatcgcggg agaagacacg gatctcctta tcgtcgacct
     8881 caacctaccc gacatgaatg gcttcgacat cgtggagcgg ctcgccaagt ccgagccgag
     8941 gccgcccgtc atcgtgttga cgggcagcac agacgaggat ttgaagcgac gctgccggga
     9001 tttgaacgtg ccgttcgttc acaagaacgc cgacacctgg aatgtccttg agccccttat
     9061 tcgcgagatc gtgggggcgc ggtcagcgtc gaacgcttaa gcgaacgaac ttgtccgggg
     9121 gcgaggtgcg ccgtcatccc ctctcgtcgg tgagcgcaca ccctcgacat cgccgatcat
     9181 ggccttggcg atttcgtgct cgcccatgac cacgagattg gcgcccagct tgaccaagtg
     9241 attgatctct tcttcggagt gggcccgggc gataatcggc aagaccgggt tgatgtcgcg
     9301 ggcctgctgg accacttgcc cgccttcgaa ggcgtcggga atggcgacga gcagacagcg
     9361 ggcatccccg agattggcag actgcaccac ttccggatcg gcggcgttgc cgacgagcgc
     9421 ctcgatgcct tggcttttga gatcggccac gtcgtctgcg tcgtcctcga cgatatagag
     9481 cggcgtcccg cgctcgcgca gctgcttcga tatgaactta ccgacccggc catgtcccac
     9541 caggacgacg tggtcggtga gggtcgtcgg aacgattggc tcgcgcgttg gagtcgcctc
     9601 ctcttcctcg gtctccgcgt ccatgtcggt accttcagga accacgcctt ccgcggcgag
     9661 cctctcgcgc cgggcaaaat aaccatcgag cgcggcgaac agcagcgggt tgagcaggat
     9721 cgagatgatg gcgccggcga ggatcagatc gcgtccctcc tgcggcagga caccgaggct
     9781 ctggccaaga ctcgctagga tgaaggagaa ctcaccgatc tgcgccaacg aggcggagat
     9841 catcagcccg gcgtcgtggg aatatccgaa cgcgcggacg atggaatagg cgacgatcga
     9901 tttcgccacg acgatgatcg ctacggtggc gagcaccgcc agcggctcgt ccatcatgat
     9961 ggccgggtgg aacagcatgc ccacggagac aaagaacagc accgcgaagg cgtcgcgcaa
    10021 gggcagcgac tcggtcgccg cctgctggct caactgagac tcgctcagca ccatgccggc
    10081 gaagaacgcg ccaagcgcca gcgatacgcc gaacagctcg gcggcgagaa atgcgaagcc
    10141 gagagcaatg gacaacaccg cgaggcggaa cagctcgcgg ctccccgtat gcgccacgta
    10201 atgcagcagc atggggatca gctttttgcc gacgacgaac atgacgaacg tgaagatcgc
    10261 cagcttaccg attacctcgg cgcattggcg cgccatctcc gtcaggtcgg gtggtgcatc
    10321 gccctcgaac aatccggtga tcgccggcag cagaaccagg gccatcacca tggcgatgtc
    10381 ctgaacgatc agccagccga cggtgatcct gccgcgctcg gtgtcgatca ggcgccgctc
    10441 ctggagcatg cgcaagagga ccacggtgct cgccacggac agggccaggc cgaagatgaa
    10501 cccggcgccc aggctccagc cgagcatcca gcccacgccc atgccgagcg gggtggcgac
    10561 cagaccttgc agcacggcgc ccggaatggc gatggcgcgg acggagagca ggtccttgac
    10621 tgagaaatgc aagccaacgc cgaacaggag gaggatgacg ccaacctcgg aaagctggtt
    10681 ggcgatgttc tggtcggcgc cgacgctcgg cgagaacccg atggcgatgc ccgcaacgag
    10741 gtatccgacc agcgggggca gcttgaggcg gtgagcgacg acgccgagac tgaaggcgag
    10801 cacgaggcca atcgtcagcg tggaaacgag tggcgggaca tgcagcatta tcaagaagcc
    10861 ttcggcgggg tcgccggcga gcactgtcgc cgaccttagc ctgacagcga attgtgacgg
    10921 ccacagcccc tcccgtccag aaaggaccgc ctaaaaacgc atagaatctg cggcatttac
    10981 gccgttgggc atctcaactt gacagtccgg tgccgcatct ccatgttgca gaaacattga
    11041 ttgctcgcga tcgcgcgaag ctgccacgcc tgtggcgcct catcccgaga caatttcccg
    11101 attttcgtct gcccccagat agcgcgtggg gtgaggcaag cgtcctcacg gtccattggc
    11161 gttcgcgccg gtgccgggac gccgaggaac attacttgac accttctttc gatatgttcg
    11221 gccttgccga accgttgcgg cgtgcgctta acgccgagaa ctaccaaacg ccgaccccta
    11281 ttcaggtcca ggcgattcca attctaaccg agggcaaaga cctgctcggc gtagccgaga
    11341 cagggtccgg taagacggcc gcctttgcgc tgccgctgct gcacaacctc tccggtgata
    11401 ccagcccgcg caagccaggc atgccgcggg cgctcatcct ggccccgacg cgcgaactgg
    11461 ctggccagat cgccgaccgg atcaacgtct atgggcgcca cctcaaactg cgtacggccg
    11521 tcattttcgg cggcgtgggc caaggccagc aggtcaaagc gctccgagcc ggtgtggaca
    11581 tcgtggtcgc aacgccaggg cgcctgctcg atctgtgcaa tcagcgccat attcgcctga
    11641 gcgaggttca gacgctcatc ctcgacgaag ccgaccgcat gctcgacatg ggcttcatcc
    11701 acgacgtgcg taaaatagtc ggcttctgtc ccaaggagcg ccagaccctg ctgttctcgg
    11761 cgaccatgcc gccgtcgatt gcaaaacttg cgcacgacat tctgcgcaat cccgtccgtg
    11821 tggacatctc ccgggccaag gtcgccgtcg accgcatcgc gcaacgcgtc tatttcgttg
    11881 gcgcgcggga caagcgcaca ctgctgaccc agctggtcac ggacccggca ctaagccagg
    11941 cggtcgtgtt tacgcggacc aagcgcggcg ccgaccgcgt gtgccgtcat cttgtcgagg
    12001 caggactcac cgcggcggcc ttgcacggca acaaggcgca aaatgcccgc gtgcgcgcac
    12061 tggacggctt tcgttcgggc cgcgtgcaaa tcctcgtcgc cactgatatc gcggcgcggg
    12121 gcatcgacgt tcctggcatc tcgcatgtca tcaactacga gctgcccaac gagccggaga
    12181 gctatgtgca tcgtattgga cgcacggctc gcgcaggcgc acaaggcacc gccctgtcgc
    12241 tctgcgacaa cagcgagcgg ccttacctca aggccatcga gcggctgatg cgcgtcgatg
    12301 tcagtgtggt ggaacacacc ctagcggaac gcgaggacac ctcgccgcgt ccgggcaacc
    12361 gcaacggtgc caagaggcat gccgccaaga agcgccggac caatcggggc cgccgcccca
    12421 accgagccgc gggcaagagc cgcggtgcaa accagcaaca ggaagtacga ctatgaacaa
    12481 gacaatctca cgcatgtcgg cagcgcggcg cagcgccgag gcgatcctga gccaaagcca
    12541 gaagcggcag gaaagcttca agcgcgagca gcagcaggcg agcgatgcct tggcggagaa
    12601 gaccgcgcgg ctacgcgagc ttcggctggc caaggaagcc gccgatcgcg aagccgaggc
    12661 cgcggtcgcc gcagcagcac ctaagccgaa gaagcgcgcc gcggtgcgcc ggtccaaaca
    12721 gacctagaca cgggaggccc tttagaagat ggcgttcaaa ccgaactaca atttccagaa
    12781 ggctgagcgc gcccgagcaa aggcgcagaa gaagcaggag aagctgcggc gcctcgaaga
    12841 agagaccgca aagcgcaagg ctctccggga cggcgtgcct gagaccggca cgccggacga
    12901 ggatgccgga acgcccacgg acgacgctta acccgcggga cagcaccatg gccaggaagc
    12961 agcaagcgcg cccaagcggc tttgtcttgt tcgacgtcat ctacgaggac ggccggcaga
    13021 catcgaaccg gaaggtcccc gccaccgagc tcggcggctt ggacggggac gcccccgcgc
    13081 gcaccattat cgaggctcag gatcagacga ttgccgaaat gtccggcaag ccccgttccc
    13141 cgatcaagtc attggcccgc tcgcgcaaag ccaaggctaa agccaaagcc tcctagccaa
    13201 agcggtttca tcgccgcgct cctcgactat agtgtggctc ccgccgcacc tgcggtgtcc
    13261 ccaagccgcc ggtcgaggcg cattctggcc cggaggaaaa ttcatgcaaa ttcttgtcgc
    13321 cgccgtgaag ggcgtgttgg cgttcagcgt tgcgatgctc tttgttgacg acgcgagagc
    13381 gcaggttccc atcgaggaag acgccggcac gatcacgatg accggcaccg tcgaagaaat
    13441 caaagtccct gagcgcgtcc tgatgatcgt cgggcccggc ggcaggacga tcgtcggcgt
    13501 gatcagccca gacgtgaagg acatcaagac gatcaagctg aaggagaagg tcaccatcca
    13561 gtttacccag gaggtggccg tggcgctgcg caaggccgat ggcgtgccgg aggcggtcaa
    13621 gaataagttc gcagagagcg aaaccgccga tatggggatg aacgcgccga cggtggccga
    13681 gcagagctgg accaatctca cgccatcggg tggcacggcc aatctcacca cgatcgaact
    13741 caccgccacg atcgcggcag tcaataagaa ccagcgcacc gtgtcgttcg ccggaaccgg
    13801 cggcatgacc cggaccgtct atgtgccgcc gagcattgtg ggcttcgatc aaatccaagt
    13861 cggcgacagg gtggtcatcg agatcacgcg cacgtcgatc gtcaacgtca aggcgttgta
    13921 gtgcgcgacg aaagccaagc ctgaaacgac aaaggcaggg agattgctct ccctgccttt
    13981 tttgtgtccg atgctgcgcg cggcaagcta catggtcgaa accccgacca cctcgtgatc
    14041 ggtgatcacg cagttccagg cggcgacggc cgggaactca cgcagatcct gccgctgccg
    14101 caagaactgc atggccgcga gatagccgcc atgccccaag caagcgttcg gcgacatgcc
    14161 ctcggcgagg aattccatgg gcacgggcac gtcgatgcag ccggtcgcga tcgcggcggc
    14221 catgctctcg gcgcgcgtca cgggcttttc gttgttgtag tccttgccgt ggttggtggg
    14281 ctcgagtggc accgtcacgc cacggcacgc cttgacgatg agctggacga aaacagtctt
    14341 ttccttgtcc tcccccatgc gggtcggccg gtcggaggcg tcttcggcga cagcggggcc
    14401 gcacatcagt gcacccagcg caagcgcggc gattacagga cgaatcatgg tggtcgtctc
    14461 tcggtcttga gagctggcgg ccgcgctctc ttcgagaacc tcgcatcgcg gctggccggt
    14521 gccgctttgg gctcacgccc ttcccccaac tgggaagtat tctagcggtg cctgccaaga
    14581 ttcccaaacc atgtgcttgg caattcgggc gggaaacgac cgtttttcct caggacgctg
    14641 gcgttcgggg tcacgatgcc gtgacggcct ggcagccata ttcggctgcc gatgccttca
    14701 gccagcgcca gaagctgtcc gccatgctgc ggttgaggac gatgttgaag ctctccgcct
    14761 cgccctcccg ccagagagcc acgcccatat gcgaaagcgt gctggtggcg gcgctgccgg
    14821 ccggaaagac gcgcaggtcg aggtcgatgg ccagcccctt ggcaagacaa tcgcgcgcgc
    14881 gcggcccgct gaaacgcaaa gccccacggc cgctgctctg atcggcgacg gacgcgcacc
    14941 cttcgagctt ggcggcaaga tgcgctgcca ggtcttcgtt gccaatcgtc tcagacacgg
    15001 caagccattg gccgggaccg gtgccgatga acatcacgtc tttgccgaac gccacggtgg
    15061 agccctcggg cagcgccgcg ccataggcat cgcgcacgca tgatttcagc gcatcggttt
    15121 gtccttcgcg gacctcgaga ctggcgaggc caagccccac gcgctcagtg acggtcacgc
    15181 ccgcctcgcc gtctttggcg ccgatgcggc ccggcgtgag aatgccctca aaggcggagc
    15241 gggatgtcag cttagacacg gacacgctcc ccatccggat cgacgaaatg cggtgcgacg
    15301 acctcggcca gcatctcggc cttgcgcaac ggatcggcga ccttcaccac ctcgccgtgg
    15361 cgtgcggggc cgttctccag gcccagcgct atgtcaacta aaaccctcag tattactgca
    15421 caccgcaaaa ccgattgcaa ccaattaatt ctttacgggc ccaaagtggt acgttacatt
    15481 gccgccgact atcgcaaagc tacgaggtca caccgtgagc gaccaaccga cacgggatga
    15541 agaaaccatg ctggcgcgct tgtcgaagcc gacctctggc gtgggcacca tcacagcgta
    15601 tttgcagcgc gcgatcgaaa ccggcgcgta tagcgagggg gacaagcttc cgcccgaacg
    15661 ccagctcgcc gaaaccttca aagcggcgcg ctcgaccgtg cgccgtgcgc tcgacaggct
    15721 ggagcgggac gggctggtgt cgcgccggct cggcagtggg actttcgtgg gcgcctccgc
    15781 ggtagccggc cagcgtcccg cgggtctagt cgatgaactc agccccttgc agctcatcga
    15841 agcacggctc gcggtagagc cgttcacggc gcggcttgcg gtcctgcacg ccacgcgacg
    15901 tcatctcgac gacatggacg ccatcctggc caaagcggag gccgcagcgc atgacaaaga
    15961 cgagttctcc aaatgggacg gcgagctcca tctcctcatc gcgcgcgcct cggccaatcc
    16021 gctcctgatc agcgtctacc gccagatcaa ccatgcgcgg ctgcacgccc agtgggacgc
    16081 catgaaggag aagatactaa cgccggatgt gatagacgaa tacaatcgtc agcatggtgg
    16141 cattctccgc gccatccacg tgcgcgatgc gcagcttgcg caagcactga tggctgagca
    16201 tctggagaag gcgcgcgacg atctgttgag ggcggcaaac ccttaagtag cgggccaatg
    16261 ccaactccgg acggcagccg ccgttaacac tacggcccat aagactgcac ctgagcgcgg
    16321 tctttgcaac aaatcccagt taccttggct ggccatcgct ggcaccgaca tcgttctctc
    16381 tctaaaatcg gcgtgaaacc gaggcgagaa ccaggctaac cctttctgct tcttaatgtt
    16441 gccggcccag tgcttccatt acgatgggtt tcgagtggcc gatgacgaga gtaatgatgg
    16501 atttccgttg cagatacatg agacccgccg agggtcttac caagcgggcg cacaggctgg
    16561 tcctaaccgg tctttgcgtc acggccgcga cctgtcttgg cgtgcccgcc ctggcgcaaa
    16621 ccgtcccccc ggtcgagacg gaagtggtgc gcgaggcgcc cgtggtgcgc aagctggagc
    16681 tctctggcac ggtcacctcg ccgcatgcct cgcagatttc cacctcggta ggcggcctgg
    16741 tgagcaaggt ctatttcgac agcggcgcgc atgtgaagac cggcgatctc atgctggagc
    16801 tcgacgccga gcttgaggag gccgcgtacg agcaggccga ggcccaagcg gcgcaagccg
    16861 ccggcgaggt caaggacgcc aagcgccgct tgcgcatcgc cgagagcctg gccaagcgca
    16921 aatacggacc acaaaacgaa gtcgaggcca ggcaagccga agtcgacatc gacacggcga
    16981 cgcatgagag tttcaaggcc gagcagaagc gccgccaagt cgttcttcag cgccaccaac
    17041 taccggcgcc ttacgacggg gtgatctcca aacgcatggc cgaggtcggc cagtgggtga
    17101 cgccggggac ggccgtgttc gagctcgtgg ccatgcacga cttccgcgtg gatgtgcccg
    17161 taccgcaaga ctattacgcg cgattgcggc agggcgcgga cgttaggatc gaaatcgacg
    17221 cgctgcccgg cgaactcgtg ccggcaacga tcggcgcgct gatcccggtg agcgatccgg
    17281 atgcgcgcac gttcaccttg cgggtcctcc caaacgcaac gacatcccca tcacgcccgg
    17341 catgtcggcc cgcgtcctga tcgatctcga cacaggcaag caggacgtgg cggtcaaccg
    17401 tgacgccctg atccgctatc cggacggacg catcaccgtg tgggtcgtga aacaagacgg
    17461 cgatacggtt agcgtcaccg agcggcgggt ggagatcgga cttgccttcg atggacgcgt
    17521 tcaggtcctt aaaggcctga aggaaggcga ccgcgtcgtg gttcgtggca acgagtcctt
    17581 acgcgaaggc cagaccgtca agctggcgag ctgaggcaag acacgtaaag cacaatgctc
    17641 gagcagatcg tcaagcgcgg aaccctggtg acggtcatcg ccctgatcgt gtgcgtgctc
    17701 ggtatcgttg ctgccttccg tatccccgtg cagatgattc cagatctcga ggtgcgcacg
    17761 gtcacggtgc acacgcgctg ggccggcgcg acaccgcagg acgtcgagaa agagatcgtt
    17821 atcgagcagg aggaatatct ccgctcgatc ccaagccttg aacgcatcac ctcccgcgcc
    17881 tccagcggcc aagcacgcat cgagatggag ttcccctaca atatcgacct caacgagacg
    17941 ctcatccgca tcaacaacgc gctgagccag gtcccctctt atccggagga cgtggaccag
    18001 ccgcgcgtgc gcgcaagctc cttcagcgcc aactccttca tgtatttccg tgtctcgccg
    18061 ctgccgggta atccgcgcca gctaaacatg gacatgatgc gcgacttcat cgacgacaat
    18121 gtccgggcgc ggctggagag cgtcacggac gtgtccgatg tggggttcgg cggcggcgcg
    18181 gaaaagcaga ttcagattct gttcgatccg gcggcgctgg ccgaacgaaa gatctccctc
    18241 acggatgtgc gccgcgcggt ggcggcgcgc aaccgcgata cctcgggcgg cgagatcgaa
    18301 agcggcaagc gacgctacct gctgcggacc atcggccggt tcgacaatcc agaggacctc
    18361 aaggagctga tcatcgcccg gcgcggtgac aacatcatcc gcctcgagga cgttgcggac
    18421 gtccggctcg gacattttga gatctacaca cgtgcctatg tgaacgacca gcccatcatc
    18481 ttcctgtcga tccgtcgcga ggccggctcg aacgtcatcg ccatcaagga ggcggtgatg
    18541 aaggaggtcg aggagatcaa ccgcgaagtc ctgatcccca cggggatgga gctggagctc
    18601 acggcggacg acgtcaacta tgtcgaagcg tctatccgca atgtgtggac gaacctgtcc
    18661 atcggcgcag tgctggcgac gctggtgatg tggctgttct tgcgctccgc caaggcaacc
    18721 gtggtgggcg tgatcggtat tccgatctgt accatcgcgg cgtttatcgg gctgatggtg
    18781 gcagggcgga cggtgaacgt catttctctt gcgggggttg ctttcgccat cggcatgacg
    18841 ctcgacaaca ctattgtcgt gctggaaagc atcgacctgg aacgccgcaa agggctggac
    18901 cggctgaagg ccgccatcga aggcgtgcgc aaagtttggc ccgcggtgct cgcctcgaca
    18961 ctgaccacgg tgctcgtgtt cgtgccgatc gtgttcatcc aagaagaagc cgggcagcta
    19021 tattccgaca tcgccatcgc cgtgtcggcc tcgatcctcg cctcgatgat cgtcgccatc
    19081 acggtcgtgc cgacggcggc tgcgcatttc gacctgacgc cgcaagacaa taaggccgtc
    19141 gatcgggcag acggcgccat tgtcaaaggt gttggttggc tcgtgggcgg gccgatccgg
    19201 cgtttgctct gcatcttggg tgcggccggc gccagcgcgg cgatcattct gctcctgacg
    19261 ccgccggccg aatatctgcc ggagggcgag gagcccaaga tgttcgcgcg cctcagcgca
    19321 ccgcccggct acaacctcga caccatgact gagatcggca cggatctgca ggactggctg
    19381 ctgcctttcg tcaaggacga tccggcgcgg ttccatcgcg gcgagaccga agtcccggcc
    19441 atcaagtact tcttcttgat gatggaggcg gactcccttc gcatcatcgc cgagacgatc
    19501 gatcccggcg atatcggcaa gctgatgaag gtcgtcggcg agagatatag gacctatccg
    19561 gggatgcggc ccttcgtgtc gcgcggctcg atcatcacca gcaatgacgg cggcacgcgc
    19621 agcgtcagct tggatatctc gggcccgcgc cttcaagaca tctacaacgt cgcctccacg
    19681 gcctatcggc gcgccggcga gatcttcgac acgccacgta ttcaagccga gccttcgacg
    19741 ctgaccctgt cgcagccgct gatcgaggtg cgccccaaat gggaccgcgc cgccgagctt
    19801 ggcatgacgt cggacgacgt cggcttcacc gtcgcggcac tgacggacgg cgcctatgtg
    19861 ggcgagttct tccgcgacga cgagaagatc gacatctatt tctacagcaa ggatgccttg
    19921 cgcacctcgc tcgacacact gcccgacatg cccgtctata cgccgcaagg caccatcgtg
    19981 ccgctatcgt cggtggccga catcaaggag acggtggaca ccaactcgat ccggcgcatt
    20041 gacggcaagc gtacggtgac actcaacatc ataccgccgg acgacgtggc gctggagacg
    20101 ggcgtggaaa ttgtaaagag ccaactcgtc gatcacatga agcgcgccgg cgatgtgccg
    20161 ttcgatgtga gcatgacgat ctcaggcgcc tccgatcagc tcgacgcgac gcgcgaggcg
    20221 ctggctggaa actacatcgt ggcggtcgtc atcatctatc tgctgctcgt cgcgatcttc
    20281 acccattggg gctatccgct gctcatcatg accgctattc cgctcggcgt ggcgggcggt
    20341 ctcgtggggc tcgcactgat gaacttggtg ggcgcgatcc tgccgcagtt cggggtcgct
    20401 ccgatcatcc agcccttcga catgatcacc atgctcggct ttctgatcct catgggaacc
    20461 gtggtgaaca atccgatcct gatcgtcgac cgcgcgctct acaatctgcg cgagatggga
    20521 ctcgatgcga aggacgcggt gatggaggcg gtatcgtcgc ggcttcgccc tatcgcgatg
    20581 tcgacagtga ccacgatctg cggcctggcg ccactcgtgt ttattcccgg cgccggaacg
    20641 gagctctatc gcggcgtcgg cgccatcgtc ctgttcggca ttctgagcac ggcgatcatc
    20701 acgctgacct tcctgccggc gctgtccatc accgtgttcc ggctcgccgg ctatggcgcg
    20761 aaggcaaatg cgaaacgggg ccggatcgag gcctcgcccg ccccaggcga gtagcggcgt
    20821 aagcccacaa tagacatcga cggaaccagc tctaatgtcc gctatgtgcc aataggagac
    20881 aaccacgcgg gatgacgcgg ctaaccagac ctcagtctag caattgcgct ggaagctgtg
    20941 gtctgggatc tttgtcagat ccttcgtgaa ggtgtccgtg aggactttgg caagtgtcgc
    21001 cggcaacgcc tgcctgacct cacctagcac cttcggcggc gctaccagaa cgaattcagg
    21061 acgccctttg ctggcgctgt tgtgccgctt cctgaacaaa agtgggccag aacttgcgca
    21121 aatctcgcgg gaacatcacg gcaattttta tagccgcgcc gcgatcaaca tacttcgaca
    21181 gcacgtcgag cacggccagc gctccctgct ctacttcgtc ttccgtcgca cgaggcaact
    21241 cctgcgcgat gtgcgcaagg aacgcttctt tatggcgctc tttggtcggc ttgtttgtcg
    21301 ggtcccagcc ttcatagtag agcccgcgca acagcatagg cagctgtgcg ccgagatgcg
    21361 ccgcgctctc tggaccgata cgatcacgaa gagcgtgtaa caccgcccgc aagcacgagt
    21421 aggcgcgatg gcggtctgtg gtgttaagcc gatccattac agctttgagc cagcgattgg
    21481 actcctgaac ggtcgtatca aaagtatcga ggccggtggt catgggcttt cctccttgct
    21541 gctcgaacta agctcaagac tcagggcgtc tggagctcct tcgaccacgc atcatgatta
    21601 ttcggaaggt tgaggccaaa agagaagcac cagtacaatc gtccaaataa tgccccaaaa
    21661 aatccaagtg atgttcgggt gatccacctg ccactctagg atgcgcttgt ggatgctcat
    21721 aaaaatgatc tcatcttccc gaggctccta ttttgggacg agcttgggtc ttagcccttc
    21781 ttgatctcga tcttcttctc gcgcttttgc gcatcgtccg acttgggcag aagaaccgtt
    21841 aggacgccgt tcttgacggt tgcctcgatc ttgtcgggct caatgccatc gggcacgcga
    21901 aacgaacgct gaaaggcgcc gaagcgacgc tcggaacgat agtaatcttt ctgcttttct
    21961 tcggactcct ccttcttctc gcccttgatc gtaagcacgc cgtcggcaaa ctttacgtca
    22021 atgtccgtct cttccatgcc cgggagttcg gccgtgactt gatagctttt gtcatgctcg
    22081 acgatgtcga cggcgggtgc ctgggcaaga ccgaactcgc cgcgccaaaa tggttccgta
    22141 ctcaacatgc cgcccggtcg ctgacccgaa acggcactga aatcatcgaa tagtcggtca
    22201 aactcacgcc gtagggtcga tagtggatcc catacaccga cgtgacgggg gctttgctcc
    22261 ggctttgccg gagctttgga tgatttgtca gccatctcct tactcctgct cacaactggg
    22321 gtaggagaaa tcatcaccga ctgcgccctc tgtcattgag ctgcgtcaag atcccaactt
    22381 ctcccagcgg cgcaggtcag ctgttttgat gagaacgttc cgacccagtc gcgagccgca
    22441 tgtccgcttt gggtcattag cagacattgg acgtctatcg aacgatgtcc gctttacccc
    22501 ctaaagcaga aatcccgagc gtcgaaatcg atgtccgtaa agtgccagaa gcggacatca
    22561 atgccgcact ctgcgaaacg tgtgcgcgtc tcgaggacat gagacgattg ctggcgcgtt
    22621 gaaaacaatg ctgaccaacc cggctttgac tttcctggtg gtcgtccgtg agcatccagc
    22681 ttagaaagac cagctttctc caaaccctcc ttgatgtgct ccactaagct gcagtttggg
    22741 acgtaggagg ataggaatcg ctcaggattc ttcgggaact gatgattgag caggacgagt
    22801 tgttgcagtt cgagggaggc ggccgtttta agcccgagct gggcgagtgc cgcagcgcgg
    22861 tcaatatagc cccagtacca gtctcgcatt ccgaagcgca tcgctgcgtg tagcgcgtct
    22921 tcatattcac cattcgcata ggagacaacg aaacgagcgt ggtggatcca gccgggaagg
    22981 cccgtctgat cgaacaggtc ccactctggt cgatcgccaa agtctgacgg atccgaaacc
    23041 aaagccagca tccaagtccc ctgtgcccga ttgattcttg aattgtgggg ctccgttagc
    23101 gcccgaccga tctgtgcaac cgcgtcttta ttgcccagat gcaacgccaa gaaggaatca
    23161 acgaactgga cgcgcgcatt gtctggacgg gcatctttgg cacgatcgat caaatccacc
    23221 acatactgca cggcacgcgg atcgtcatcg tatccgtgct tgtggccatc aaggaggaga
    23281 tcggcatacg cggcaagaag atcgggatga tcagggtggc tggtgagctc aacctgaacc
    23341 gcggctgaag cgcgttgatg ggccgccatc gtcgcaatgc cttgatacca atctgcccac
    23401 cgcaaagcgg tagcagggcc attgtcctca tctgatcgtg cgtggctgaa tgagggcaca
    23461 tagcagccgg gcgcaaaccg gataacgatg tcgtccttct ggccttcggc cgagtagtag
    23521 agcgcaagcg cattccgcaa acgattgaag gcaaccctga cactggagtc cttggagctt
    23581 cgaaacttca aacccttacc gagtgcgaac cgcccgacag tagttgcgcc aagttgtgcg
    23641 gactcgccgg ccagggttga ctcgacggta taccgaagta tgcgctgcaa aacaggggcg
    23701 tcatgaaaac gttggctgcg gcatatctcg gacacctgcc gcattatcgt ttcggcgtcg
    23761 gatgacggtt gaagatctcc caagccagta ccctcccgtt gctcggcgat cggattataa
    23821 ggccccttgg gtgttagtaa cagcttagct aaggatggag tttttgttag cctcaaaccg
    23881 ccttgatgct tgcccaagca tgacgaccgg gagaactagc atgctgagaa ccgcttttgt
    23941 tgcgctcatc cttctcatta caacctcagc aggtgtggca gctgagacgg ccgactcggc
    24001 atacattaat ggcaagatct acacggtgga cgcgacaaag ccttgggccg aagcagtcgc
    24061 cataaaggac ggcaagttta ttgcggttgg cagcaatgaa gcgatcgcgg ccgtcacggg
    24121 cgaaggcacg aaagtcgtcg atctcgatgg tgggtttgca atgcctggca ttcacgacac
    24181 gcatgtgcat ccaccgcttg tctacggtca cgaggaggca ggccgtctac tattttctga
    24241 gacgaactcc ccggacgaaa taatcgagat cgtgaaggag tacgcagcaa agaatcccga
    24301 tatcgagatc attagaggag aaaagtgggc tgcgggcgat ttcccgggag gcaaggccac
    24361 aaa
//
LOCUS       DBNHVU010000184         4272 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_213642, whole genome shotgun sequence.
ACCESSION   DBNHVU010000184 DBNHVU010000000
VERSION     DBNHVU010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_213642"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..1683
                     /locus_tag="AC16Y7_07840"
     CDS             10..1683
                     /locus_tag="AC16Y7_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HIR5709329.1"
                     /translation="MAMRSRTPETGDDTEDETATTKPGKRAAKKKRAAKRPRKTARKS
                     AKKKAVPKAAEAVADPAPIELPDDCVFIGKSDKPEYILLKLANRHGLITGATGTGKTV
                     TLQGLAESFSDQGVPVFCADVKGDLSGVAEMGVEKGWIKERAAMLGYEVPYSAHPVIF
                     WDLFGEQGHPIRTTVTELGPLLLSRLMDLSDAQEGVLNIAFRIADDEKLPLLDLKDLQ
                     SLLQNLASRAKDLTTKYGNVSKVSIGAIQRSLLVLEQQGAEYFFGEPALDVRKDLMRK
                     DSDGRGYISVLAADKLMQSPRLYATFLLFLLSELFEELPEVGDPKKPKLVFFFDEAHL
                     LFRDAPKVLLEKVEQVVRLIRSKGVGVYLVTQNPRDIPDTVSRQLGNRVQHALRAFTP
                     SEQKAVKAAAETFRENPDIDTETAIMELGIGEALVSTLDKKGQPSMVEHTFIKPPSSQ
                     VGPLSPKQRRAIIEDSPVAGVYDEVLDRKSAYEILKKRTEQRAKELAEAEAADEKTKT
                     SRKKSGGRRGDSFWTALGKSVVRSVVPMATRVLEDAIKRNTMGGISRRR"
     gene            1723..3597
                     /locus_tag="AC16Y7_07845"
     CDS             1723..3597
                     /locus_tag="AC16Y7_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR5709330.1"
                     /translation="MFTLDAISLRLGGHVILDRASAALPPRSRVGLVGRNGAGTSSLL
                     KLIAGVYELDGGRIEAPSGTRIGYVAQEAPGGSATPFETVLAAAEERAALLAEAEHAM
                     DAHRIAEIHERLNAIDAHGAPARAARILAGLGFSEEAQHQPLETFSGGWRMRVALAAL
                     LFSEPDLLLLDEPSNHLDLEAALWLESFLKTYRNSLIVVSHERDLLNHVVDYILHVDH
                     GKATLYAGNYDQFERQRRERQAQAAAARASQEAKRKKLQAYVERWRYKAHTARQAQSR
                     LKALSRMEPIAEALDDASLVFDFPSPKETKPPLLLLEDASVGYVPDTPVLSGLDLRID
                     PDDRIALVGRNGNGKTTLARLLAKQLAPMTGRIAASGKLTVGYFAQHQIEELVADDTP
                     LQHMERLLPGAKPGEVRNQLARFGFSGDKVSVAVRQLSGGERARLSLALITRQAPHIL
                     ILDEPTNHLDVDAREALVQALAAFAGAVIVVSHDRHLLGLIADRLLLVADGTVREFDG
                     SLDDYRDSVLSVAKSGKGNGKAPGPKVNRKEKRKAAADARERTKALRKAVVQAEADMK
                     ALTVRRDALDADLVNAAPTEASALMKQRGALTRDIEEAEARWLEASEAVEAAMSEVYA
                     "
     gene            complement(3649..4257)
                     /locus_tag="AC16Y7_07850"
     CDS             complement(3649..4257)
                     /locus_tag="AC16Y7_07850"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIR5709331.1"
                     /translation="MAFELPPLPYAYDALQPYMSAETLEYHHDKHHKAYVDNLNKLIK
                     DTGYEGKDLETIVKESFGKNAGVFNNAAQDFNHIQFWPWMKKDGGGKKIPGSILALID
                     RDLGGYDKFRGDFIQAGMTQFGSGWCWLTLNKDGKLEVTKTPNGENPLVHGGWPILGC
                     DVWEHSYYIDYRNARPKYLEAWFDHLINWDHVEELYAQAPTS"
BASE COUNT          819 a         1275 c         1415 g          763 t
ORIGIN      
        1 gagagggtca tggcgatgcg gagcaggacg ccggagacgg gcgacgatac ggaggacgag
       61 acggcaacga cgaagccggg aaagcgggca gccaagaaga agagggccgc gaagagaccg
      121 cgaaaaaccg cccgtaagtc cgccaaaaag aaagccgtgc cgaaggccgc cgaagcggtg
      181 gcggatcctg ctcctattga gttgccggac gattgcgtct tcatcggcaa gagcgacaag
      241 cccgaataca tccttctgaa gctcgccaac cgccacggac tcattaccgg cgccacgggc
      301 acgggcaaga ccgtgacgct gcaagggctt gcggagagct tttcggacca aggcgtgccg
      361 gtattttgcg ccgacgtgaa gggcgacctc tcgggcgtcg ccgagatggg cgtggagaag
      421 ggctggatca aagaacgcgc cgcgatgctc ggatacgagg tcccgtacag tgcccatccg
      481 gtgatcttct gggacctgtt cggtgagcag ggacacccca tccgcacgac cgtcacggaa
      541 ttggggccgc ttcttctgtc gcgcctgatg gatctcagcg atgcgcaaga aggcgtgctg
      601 aatatcgctt tccgcatcgc cgacgacgag aaactgccgc ttctcgatct gaaggatctg
      661 caatccttgc ttcagaacct cgccagtcgc gcgaaggacc tcaccacgaa atacggcaat
      721 gtcagcaagg tttcgatcgg cgccatccag cgctcacttc ttgtgctgga gcagcaaggc
      781 gcggagtact tcttcggcga gccggcgctg gacgtcagga aggacttgat gcgcaaggac
      841 tcagacgggc gcggctatat cagcgtgctc gcggccgaca agctgatgca aagcccgcgg
      901 ctctacgcga cgttcctcct gttcctgttg tcggagctgt tcgaggagtt gccggaagtc
      961 ggcgatccca agaagcccaa gctcgttttc ttcttcgacg aggcgcatct gttgttccgc
     1021 gatgcgccca aggttctctt ggaaaaggtc gagcaagtgg ttcgcctcat tcgttccaag
     1081 ggcgtcggcg tctacctcgt gacgcagaac ccgcgcgata ttcccgacac ggtgtcgcgg
     1141 cagctcggca accgcgtgca gcacgcgttg cgcgcgttca cgccttcgga gcagaaagca
     1201 gtgaaggccg cggctgaaac gttccgcgag aatccggaca tcgataccga gacggcgatc
     1261 atggagctgg ggatcggcga ggcgctggtc tcgaccctcg acaaaaaggg ccagcccagc
     1321 atggtcgagc acaccttcat caagccgcca tcgtcgcaag tgggcccgct gagcccgaag
     1381 caacgcagag ccatcatcga ggatagccct gtcgccggcg tctatgacga ggttctggac
     1441 cgcaagtcgg catatgaaat tctgaagaag cggaccgaac agcgcgccaa ggagcttgcc
     1501 gaggccgagg cggccgacga gaagaccaag acctcccgta agaagtccgg cgggcggcgt
     1561 ggcgacagtt tctggacggc gctcggaaaa tccgtcgtgc gctccgtggt gccgatggca
     1621 acgcgggtgc tggaggacgc tatcaagcgc aataccatgg gcggcatttc gcgccgccgc
     1681 taagcgcgca gactctcatc gtcctgtgct atcacgccca ccatgttcac gcttgatgcc
     1741 atctctttgc ggcttggcgg ccatgttatc ttggaccgcg ccagcgccgc gctgccgccc
     1801 cggtcccgtg tggggctcgt gggacgcaac ggggcgggga cgtcgtcgct cctaaagctc
     1861 attgccgggg tgtacgagct tgatggcggc cgcatcgagg cgccgtcggg cacgcgcatc
     1921 ggctatgtgg cgcaagaggc gccgggcggt tcggcaacgc ccttcgagac agttcttgcg
     1981 gcggcggagg agcgggccgc gcttctggcg gaagccgagc atgcaatgga cgcgcaccgc
     2041 atcgccgaga tccacgagcg gctcaacgcc atcgatgctc atggggcgcc tgcgcgggcc
     2101 gcgcgcatcc tcgcggggct cggcttctcg gaggaggcgc aacatcagcc cttggagacg
     2161 ttttccggcg ggtggcgcat gcgtgtggcg cttgcagcgc ttctgttctc cgagccagac
     2221 ctgctgctgc tggatgagcc ttcgaaccat ctggacctgg aggcggcgct atggctggag
     2281 tcgttcctca aaacctaccg taacagcctc atcgtcgtga gccatgagcg cgaccttctg
     2341 aaccatgtcg tcgactacat cctgcatgtg gaccacggca aggcgacgct ctacgcgggc
     2401 aattacgacc agttcgagcg gcaacgccgc gagcggcaag cgcaagcggc ggcggcgcgt
     2461 gcgagccagg aagccaagcg caagaagctg caggcctatg tggagcgctg gcgctacaag
     2521 gcgcatacgg cgcgtcaggc ccagagccgt ttgaaggcct tgtcgcgcat ggagccgatt
     2581 gcggaagcgc tggacgatgc ctccttggtc ttcgacttcc cgagcccgaa ggagaccaag
     2641 ccgccgcttc tcttgcttga ggatgcgtcg gtcggctacg tacccgacac gccggtcttg
     2701 tcaggccttg acttgcgcat cgatccggat gaccgcattg cgctggtggg acgcaacggc
     2761 aacggcaaaa caacgctggc gcggctcctt gccaaacagc ttgctccgat gacgggcagg
     2821 attgcggcaa gcggcaagct caccgtcggc tacttcgcgc agcaccagat cgaagagctg
     2881 gtggctgacg acacgccgct tcagcacatg gaacggcttc tgccgggtgc aaagcccggc
     2941 gaggtgcgga accaactcgc acggttcgga ttttcaggtg acaaggtgag tgtcgcggtg
     3001 cgtcagctct ccggcggtga gcgagcgcgg ctctcactgg cgctcatcac gcggcaggcg
     3061 cctcatattc tcatccttga cgagccgacc aaccatctcg acgttgatgc gcgcgaggcg
     3121 cttgtgcagg cgctcgccgc gttcgccggc gccgtgatcg tcgtgagcca tgaccggcac
     3181 ctcttggggc tgatcgccga ccgtttgttg ctcgtagccg acggcacggt gcgggagttc
     3241 gacggtagct tggacgatta tcgcgatagc gtgttgagcg ttgccaagtc cggcaagggc
     3301 aacggcaaag cgccgggccc gaaggtcaac cgcaaggaga agcgtaaggc ggcggcggat
     3361 gcacgcgagc gcaccaaagc tttacgcaag gccgtcgtgc aggccgaagc ggatatgaag
     3421 gcgcttactg tgaggcgcga tgcgctcgat gcagacctgg tgaatgcggc accgacggaa
     3481 gcgagcgcgc tcatgaagca gcgcggtgcg ttgacgcgcg acatcgagga ggcggaagcg
     3541 cgttggctgg aagcgagcga agcggtcgaa gccgccatga gcgaggttta cgcgtaagca
     3601 aaacgggcgc cccgaaggac gcccgctgca atcgtttgtg tccgcgcgct aggaggtcgg
     3661 cgcttgcgcg taaagctcct cgacatgatc ccagttgatg agatggtcga accaggcttc
     3721 gagatacttc ggccgcgcgt tgcggtagtc gatgtaatag gagtgctccc aaacatcgca
     3781 accaaggatt ggccagccgc catgcacgag cgggttctcg ccgttcggcg tcttggtcac
     3841 ttcgagcttg ccgtccttat tcagcgtcag ccagcaccag cctgaaccga actgggtcat
     3901 gccggcctgg atgaagtcgc cgcggaactt gtcgtagccg ccgagatcgc ggtcgatcag
     3961 tgcgaggatc gagcccggga tcttcttgcc gccgccgtcc ttcttcatcc acggccagaa
     4021 ctgaatgtgg ttgaagtctt gggcggcgtt gttgaagacc ccggcgttct tgccgaagct
     4081 ctccttgacg atggtctcga ggtctttgcc ctcgtaacca gtgtctttga tcaacttgtt
     4141 gaggttgtcc acgtaagcct tgtggtgctt gtcgtggtga tattcgagcg tttcagccga
     4201 catatagggc tgaagcgcat cgtaggcgta aggaaggggt gggagttcga atgccacttt
     4261 cgggtctccg cg
//
LOCUS       DBNHVU010000185         5974 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_884556, whole genome shotgun sequence.
ACCESSION   DBNHVU010000185 DBNHVU010000000
VERSION     DBNHVU010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5974)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_884556"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..439)
                     /locus_tag="AC16Y7_07855"
     CDS             complement(173..439)
                     /locus_tag="AC16Y7_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709332.1"
                     /translation="MLTQLYRLVMNPSENPLRTLPPIVRFQFMVILSYMWSAIFALWI
                     GYMWLMGPSIIAHTFLLVGVFFTSEIFALASNQSKRFLQKAAAR"
     gene            728..1309
                     /locus_tag="AC16Y7_07860"
     CDS             728..1309
                     /locus_tag="AC16Y7_07860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5709333.1"
                     /translation="MSKALSVSSRPDRDEDDHELVQRVACNRDRAAYQCLFLRFGPRV
                     KALMIKSGADASMAEDLVQDVMLILWRKAALYSPERGSVAAWVFTIARNRRIDKLRRQ
                     SPQAYDDIDDLDLESPDASGEEEVHVRQQSTLVSAAVAQLPPDQKRVIELSFIHDMPQ
                     VDIAQELGVPLGTVKSRMRLAYGKLRERLEDVK"
     gene            1306..1953
                     /locus_tag="AC16Y7_07865"
     CDS             1306..1953
                     /locus_tag="AC16Y7_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ChrR family anti-sigma-E factor"
                     /protein_id="HIR5709334.1"
                     /translation="MKIEHHPDTATLASYAAGSLDEAFATVVAAHLASCAKCRARLYE
                     IEEVGGTMLETIDAVGLNEAALERAMSRLDEPSEERAEPRSAEQSVPRPLGRLVTAPL
                     DAIAWKSVAPGVAVHRLPTSKAARGSLTLLKIAPGKKIPEHGHGGMEITLVLTGSYRD
                     ALGRFGPGDVADLDEDIEHQPVVDSDEPCICLVASEAPTRFKSFFGRLFQPLVGV"
     gene            complement(1950..3173)
                     /locus_tag="AC16Y7_07870"
     CDS             complement(1950..3173)
                     /locus_tag="AC16Y7_07870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5709335.1"
                     /translation="MTDAFSQRAHQRSGTLTRLVVGAGERLLAWQPQGSIVVELPTGR
                     RISFGRDLPHDAALKLNNYRVITKSIRRGALGFADAFIASDVDCSDLVGLFRFFSRNY
                     AAFEKSGRGLFSYSVVDRIRHLLRRNTREGSRRNITEHYDLGNAFFQKWLDSQVLYSS
                     ALYSESTQTLEEAQGAKLDAILDCLELKPGMSLLEIGFGWGALALRAARDRGASVTGI
                     TLSQEQHAYASEKAKSEGADGMCRFEMKDYRDTQEQFDRLVSVEMIEAVGEDRWPRYF
                     RTIHDRLQPGGIAVLQAITIGEEHFPDYRRKADFIQRYIFPGGMLPTKSIMAEQAELA
                     GLRFEPVMNFGSSYARTLREWRRRFKARWPEIEPLGFDTRFRRKWEYYLAYCEAGFLE
                     DIIDVGLYRLTRPAV"
     gene            complement(3170..3964)
                     /locus_tag="AC16Y7_07875"
     CDS             complement(3170..3964)
                     /locus_tag="AC16Y7_07875"
                     /inference="COORDINATES: protein motif:HMM:NF018770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HIR5709336.1"
                     /translation="MMSPVTGSALYNGVVVHKRMRPVQHALRYHVFSLLLDCDELAEL
                     DQRLKLFSYNRFNVLSLHDRDHGDGSALRRYLRRIAAQSGQEIDRFLMLCYPRMLGYG
                     FNPVTVYFGLAADGRLKLMIYEVNNTFGERKSYVIPVPEDAASGIVSQGCRKSFYVSP
                     FNAVEGRYKFHLTPLGEHVTVGVALENDQGPVLKAYFRGSRSVLSDASLLVAVLRTGW
                     LSVKVIAAIHFEALKLRLKGLRLQQRPPAPRSAIDFVHPRKQNQTS"
     gene            complement(3961..5304)
                     /locus_tag="AC16Y7_07880"
     CDS             complement(3961..5304)
                     /locus_tag="AC16Y7_07880"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006725284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5709337.1"
                     /translation="MRTLNIAVIGSGISGLSSAWLLAKRHRVTLFEQDGRLGGHTNTI
                     EVETADGTVPVDAGFIVYNEQNYPNLTALFAHLGVRTDPTEMTFAVSDGGAFEYSGSG
                     LSGLFGQPSNILRPRQWRLVADLMRFFRSSRARLSLYPADISLGAFLEAEGYSPSFID
                     DHILPMGAAIWSTPMTSMLDFPASAFINFYANHGLLKVNDRPAWRIVSGGSKRYVESM
                     IADADIDVLAGSAVRRVGRSETCVHIEDNQGIVRLFDHVVMAGHADQALAVLSDPTEE
                     ETLLLGAFRYERNRAILHRDKRLMPKRRRLWESWNYMKQGRGTDSALCVTYWMNSLQH
                     LPTTTDLFVTLNPYREIHPKAVDTEILYDHPAFTNEAMAAQRKLWSLQGKRRTWFCGC
                     YFGYGFHEDGLQSGLAVAEQLGGARRPWSVPGESDRIHVTYVKPEIEHTPLEAAE"
     gene            complement(5379..5783)
                     /locus_tag="AC16Y7_07885"
     CDS             complement(5379..5783)
                     /locus_tag="AC16Y7_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009759363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HIR5709338.1"
                     /translation="MTYVVAYIATAVSFLAIDFIWLGYIAKDFYRREIGALLLENFNM
                     AAAVGFYLMFVVGILIFAVLPALQAGSWKTALIYGALFGFFTYATYDMTNLATLKGWS
                     VSVVVVDMAWGAFITGVAALAGYFATRAIVGG"
BASE COUNT         1255 a         1761 c         1835 g         1123 t
ORIGIN      
        1 ctgttttcaa ggtcgatctc attcgccgac cgagagaacg cccggacgcg gtggccggca
       61 tcgagagctg ctgaaagcgt ctctcgacca atgccaccgc tggcgccgat gacgagaaca
      121 tgcgccacga ctaccctccg atcctacacg gctcgtccgg cgactgccgc gctcaacgcg
      181 cggcggcctt ctgcaagaac cgctttgact gattactcgc gagcgcgaag atctcgctcg
      241 tgaagaagac acccacgaga aggaaggtgt gcgcaatgat gctggggccc atcaaccaca
      301 tgtagccgat ccagagcgcg aagatcgcgg accacatgta ggacagtatc accataaact
      361 ggaaccgaac aatcggcggt aatgtgcgaa gcgggttctc tgaagggttc atcacgagtc
      421 gatatagctg cgtcaacata ggaattctcc tgtattagct cttctactct gggcacgcgc
      481 ttgagctgtg tgtgctctct cgttctgttc cggttacgcg gtcatacgcc ggctggatca
      541 tgcccttcgc cggctacagg ggacacgact gtgtgatgag aggaggcggg acctgcgggt
      601 gccgcgaggg gggcggacta ccggccaggc gaaccgaacc atcatgaaag accgcaagtg
      661 atccagacgg ccggcgaagt cgtatatagt gcagagcaga aagccacggc caaacagccc
      721 taactgcatg agcaaggcat tatcggtgtc gtcccgtccg gacagggatg aagacgatca
      781 tgagttggtc cagcgcgtcg cctgcaaccg cgaccgcgct gcgtatcagt gcctattctt
      841 gcgcttcggt ccgagggtca aggcgctgat gattaaaagt ggtgccgacg ccagtatggc
      901 ggaggacctt gtccaggacg taatgcttat tctgtggcga aaggcggcgc tctattcgcc
      961 tgagcgaggt agcgttgccg cctgggtgtt caccatcgcg cgcaaccggc gcattgacaa
     1021 gctgcggcgc cagtcgcccc aggcctacga tgatatcgac gacctagact tggaatctcc
     1081 cgatgccagc ggcgaagagg aagttcatgt gcggcagcaa agcactttgg tttcggcggc
     1141 agtggcacaa ttgccaccgg accagaagcg cgtgatcgaa ttgtctttca tccatgacat
     1201 gccacaagtc gacattgcgc aggagcttgg cgtgccactt ggaaccgtga agtcgcgcat
     1261 gcgcctcgct tacggcaagt tgcgagagcg cctggaggac gtgaagtgaa gattgaacac
     1321 catcccgata cggcaacatt ggcaagctat gcggccggta gcctcgatga ggccttcgcc
     1381 accgtggtcg ccgcgcatct cgcctcgtgc gcgaaatgcc gggcccgcct ttatgagatc
     1441 gaggaggtcg gcggcaccat gctggagacc atcgatgccg tcggactgaa cgaggccgcg
     1501 cttgagcgcg ccatgagtag actggacgag ccgtctgaag agcgggccga gcctcggtcg
     1561 gctgagcagt ccgtaccccg tccgcttggc aggctcgtca ccgcgccgct tgacgccatc
     1621 gcgtggaagt cggtggctcc cggcgtcgcc gtgcatcggc ttccgacgtc caaggcggcc
     1681 cggggttcgc tcacgcttct gaagattgct cccgggaaga agattcctga gcacggtcat
     1741 ggcggcatgg agatcacgct ggtcctgacc ggctcttatc gtgacgctct cgggcgcttt
     1801 gggcctggcg atgtggccga tctcgatgag gatatcgagc accagccagt tgtcgattcc
     1861 gatgagccgt gtatctgcct ggtcgcaagc gaggcaccga cgcgcttcaa gagcttcttt
     1921 gggcggctgt tccagcctct ggtgggagtc tagacagccg gccgggtcaa ccggtaaagc
     1981 ccgacatcga tgatgtcctc caggaaaccg gcctcgcaat aggcgaggta gtactcccac
     2041 ttccggcgga accgggtgtc gaacccaaga ggttcgattt ccggccagcg ggctttgaag
     2101 cggcggcgcc actcccgcag ggtccgcgca taggacgatc cgaaattcat cacaggctcg
     2161 aagcggagtc ccgccaactc ggcttgctcc gccatgatgg atttggtcgg tagcatgcca
     2221 cccgggaaga tgtagcgctg aatgaagtct gccttccgcc gatagtccgg gaagtgctcc
     2281 tcgccgatcg tgattgcctg aagtaccgcg atgccgccgg gctggagccg gtcatggatc
     2341 gtgcggaagt agcgcggcca ccggtcctcg ccgacggctt cgatcatctc caccgaaacc
     2401 agccggtcga actgttcttg cgtgtcccgg tagtccttca tttcgaagcg gcacattcca
     2461 tccgcacctt cgcttttggc cttttcggat gcatacgcgt gctgctcctg cgacaacgtg
     2521 atgcccgtga cggaagcgcc gcggtcacgg gcggctcgga gcgcgagcgc accccaacca
     2581 aagccgatct ctagcaggct catgcccggc ttgagctcaa ggcaatcgag tatggcgtcg
     2641 agcttcgcgc cctgggcctc ctccagcgtc tgggtgctct cgctgtaaag cgcgctcgaa
     2701 tacaaaacct ggctgtccaa ccatttctga aagaacgcgt tgccgagatc gtagtgctca
     2761 gtgatgttgc ggcggctgcc ttcccgtgtg tttcggcgca gcaagtgccg gatgcgatcg
     2821 accacgctat atgaaaagag gcctcggccg gatttctcga atgcggcata gttgcgggaa
     2881 aagaagcgga acagaccaac caagtcggaa caatcgacgt cgctggcgat gaaggcatcg
     2941 gcgaagccga gcgcgccgcg gcggatcgat ttcgtgatca cgcggtagtt gttcagcttc
     3001 agcgccgcat catgtgggag atctcgcccg aagctgatac ggcggccggt cggcaattct
     3061 acgacgatcg acccttgcgg ctgccaagca agcaatcgct cgcctgcgcc caccacaagg
     3121 cgcgtcagtg taccggagcg ctggtgcgcg cgctgggaaa aagcatctgt catgatgttt
     3181 gattttgttt tcgcgggtgc acgaagtcga ttgcggacct gggtgccggc ggccgctgct
     3241 gcaggcgaag tcccttgagc cgtagcttca gcgcctcgaa atggatcgcg gcgatgacct
     3301 tcaccgacag ccagccggtt cgcagaacgg cgactaggag tgatgcgtcg cttaaaaccg
     3361 agcgagagcc ccggaagtat gccttcagaa ccggaccttg gtcgttctcc agtgcaacgc
     3421 cgaccgtgac gtgctccccg agaggcgtca ggtggaactt gtagcgcccc tcaacggcgt
     3481 tgaacggcga gacatagaat gatttgcggc acccctgact gacgatgccg gaagcagcat
     3541 cctctggcac tggaatgacg taggatttgc gctcgccgaa ggtattgttg acctcgtaga
     3601 tcataagctt cagacggcca tcggcggcca agccgaaata gaccgtgacg ggattgaacc
     3661 cgtagccgag catgcgcggg tagcagagca tcaagaagcg atcgatctcc tgcccactct
     3721 gcgccgcgat gcgccgcaga tatctgcgca gcgcgcttcc atcgccgtga tcgcgatcat
     3781 ggaggctgag gacattgaag cggttgtagg agaataattt caatcgctgg tcgagctcgg
     3841 cgagctcgtc gcagtcaagc aacagggaaa acacatgata gcgcagcgca tgctgcactg
     3901 ggcgcatacg cttatgcacc acgacgccat tgtaaagcgc ggacccggtc accggcgaca
     3961 tcattcagcg gcctcgagcg gagtatgctc gatttccggt ttcacatacg tgacatggat
     4021 ccggtcggac tcacctggga ccgaccacgg acggcgtgcg ccgccaagct gttcggcgac
     4081 cgcgagccca ctctgcaggc cgtcctcatg gaagccgtag ccgaaatagc aaccgcagaa
     4141 ccatgtgcgc cgcttgccct gcaacgacca caacttgcgc tgcgcagcca tggcctcgtt
     4201 ggtgaaagca ggatgatcgt ataagatttc ggtatcgact gccttgggat gtatttcgcg
     4261 ataggggttg agcgtgacga agaggtccgt ggtcgtcggc aagtgctgca ggctattcat
     4321 ccagtacgta acgcagaggg cactgtccgt acctcggcct tgcttcatgt agttccagct
     4381 ctcccagaga cgccggcgct tgggcataag gcgcttgtcg cgatgcagaa tggcgcgatt
     4441 gcgttcatag cgaaaagcgc cgagaagcaa agtctcctct tcagtcggat cgctcagcac
     4501 ggccagagcc tggtcggcgt gtccagccat gaccacatgg tcgaagaggc gaacaattcc
     4561 ctgattgtcc tcgatatgca cacacgtttc actgcgcccg acacggcgga cggcggatcc
     4621 cgccaacacg tcgatatcgg cgtcggcgat catgctctcc acatagcgct tgctgccgcc
     4681 cgacacgatg cgccaggccg gcctatcgtt gactttcagg aggccgtgat tggcgtagaa
     4741 gttgatgaag gcgcttgccg gaaaatccag catcgaggtc ataggtgtgg accatatcgc
     4801 ggcgcccatg gggaggatgt ggtcgtcgat gaaagagggt gagtagcctt ctgcttccag
     4861 gaaagcgccg agcgagatgt cagcaggata gaggctcagc cgcgcgcgcg acgatcggaa
     4921 aaaccgcatt aggtcggcga cgaggcgcca ctgccgtggt cgcaaaatgt tcgacggctg
     4981 tccgaaaagc cccgagaggc cactacccga atattcgaac gcgccgccgt cggagacggc
     5041 aaatgtcatc tcggtggggt ctgtgcgcac gccgagatgg gcgaacagcg cggtcagatt
     5101 ggggtagttc tgctcgttgt agacaatgaa gccggcatcc accgggaccg tcccgtcagc
     5161 cgtctcgacc tcgatcgtat tggtgtggcc accgaggcgt ccatcttgct cgaacaacgt
     5221 aacgcggtgc cgtttggcaa gcagccacgc ggatgagagg cctgaaatgc cggacccaat
     5281 caccgcgatg ttgagtgttc tcattcttcc cctggcagta tttgacgcga cgcggaggat
     5341 acggtgggtg tgctgttccg gatcactcgt gaaacgggtt agcccccgac aatcgcacgc
     5401 gtcgcgaaat agccggcaag cgccgcaacg ccagtaatga acgcccccca cgccatgtca
     5461 acgacgacga cggacacgct ccaccccttc agggtcgcaa gatttgtcat gtcgtaagtt
     5521 gcgtaggtga aaaagccaaa caaggcacca tagatgaggg ccgtcttcca cgatcccgct
     5581 tgcaaggcgg ggagcaccgc aaagatgaga atgcctacca cgaacatcag gtagaacccc
     5641 acggctgccg ccatgttgaa gttctcaagc agcaaggcgc cgatctcccg gcgatagaag
     5701 tccttcgcga tatatcccaa ccaaatgaaa tcgatcgcca ggaagctcac cgccgtggcg
     5761 atgtaggcaa cgacataagt catttcgata agtcccgcag cagtagagga ggcagtagac
     5821 gagcaagata cgaagggccg ggcgcgttgg atcactcagg cacagtcaca aagacttgaa
     5881 accgcgcccc tgatccgtgg gcgggcacgg cgcgtaagcc ttcccgttag caacgtttcc
     5941 cccaacggag gttcaacatg aagattctgc gcac
//
LOCUS       DBNHVU010000186         5610 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1405275, whole genome shotgun sequence.
ACCESSION   DBNHVU010000186 DBNHVU010000000
VERSION     DBNHVU010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1405275"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..273)
                     /locus_tag="AC16Y7_07890"
     tRNA            complement(184..273)
                     /locus_tag="AC16Y7_07890"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(237..239),aa:Ser,seq:cga)
     gene            481..1059
                     /gene="rplU"
                     /locus_tag="AC16Y7_07895"
     CDS             481..1059
                     /gene="rplU"
                     /locus_tag="AC16Y7_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR5709339.1"
                     /translation="MFAVIRTGGKQYRVAPEDIIEIEKIAGQPGDIVQLGEVLLLGGD
                     GSPKSGSPTISGASVAAEVLEQKRDDKIIVFKKKRRQGYRRTKGHRQALTALRITEIL
                     TDGKKPSKAAAKPAPKKAETKPAPKAEAKPAAKAPAKKAAPAKKAETKAPAKKAPAKK
                     AAPAKKAATKAPAKKAAAKKAPAKKAAPKKKS"
     gene            1072..1335
                     /gene="rpmA"
                     /locus_tag="AC16Y7_07900"
     CDS             1072..1335
                     /gene="rpmA"
                     /locus_tag="AC16Y7_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR5709340.1"
                     /translation="MAHKKAGGSSRNGRDSAGRRLGVKKSGGQQVIAGNIIIRQRGTK
                     WHQGDNVGMGKDHTLFALRDGVVEFATKQKGRVYVSVKPSEAS"
     gene            1402..1932
                     /locus_tag="AC16Y7_07905"
     CDS             1402..1932
                     /locus_tag="AC16Y7_07905"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5709341.1"
                     /translation="MQIKTQRLILRPPALADAARIAMLAGDYDVACMTGTIPHPYSEQ
                     MAAEWLAGALNGEEGVVLMIERDGTLVGCTGYRAFGADHAELGYWIGKPYWGHGYATE
                     AARALIAHAFDADGFAYLKAGYLADNLRSERVLQKLGFVADDEEMRDCAARGDTVHCT
                     TYRLSRADAQASLRQP"
     gene            2011..3054
                     /gene="obgE"
                     /locus_tag="AC16Y7_07910"
     CDS             2011..3054
                     /gene="obgE"
                     /locus_tag="AC16Y7_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006933730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR5709342.1"
                     /translation="MQFLDSAKVFVRSGDGGNGCVSFRREKFVEYGGPNGGDGGKGGD
                     VIVECVANLNTLLDFRYQQHFKAKKGGNGMGQNRTGAKGADIVLKLPPGTQILDEDGE
                     TLIADLTEPGQRLVLLTGGNGGFGNAHFKTSTNQAPRRANPGLPGEEMYIWLRLKLIG
                     DAGIVGLPNAGKSTFLAAVSAAKPKIADYPFTTLHPNLGVVRVGGLDFVLADIPGLIE
                     GAHEGVGIGDRFLGHVERTAVLLHLVDATADDPVAAYEVVRGEIEAYGAGLEVKPGVL
                     ALSKVDAVADDELKKKSRALKKAAGVTPLLVSAVTGEGVQDVLHALAREVETMRERET
                     ADLSRKTSQGWRP"
     gene            3042..4178
                     /gene="proB"
                     /locus_tag="AC16Y7_07915"
     CDS             3042..4178
                     /gene="proB"
                     /locus_tag="AC16Y7_07915"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR5709343.1"
                     /translation="MAAMRSEWTGARRIVVKIGSALLVDSAAGKLRTEWFDAFADDIA
                     ALRAEGKEVIVVSSGAIALGRNALGLPKGALKLDDSQAAASVGQISLAHAFEDRFRAR
                     GMVAAQILVTLGDTEERRRYLNARSTMSTLLKLGAVPVVNENDTVATNEIRYGDNDRL
                     AARIASMMSADCLVLLSDVDGFYTAPPGMDENARRLDEVPDITAEIEAMAGDVGSELS
                     RGGMVTKIEAAKIAVQAGTHMVIASGKVIKPLQTLATTGVCTWFLAHSDPVTARKRWI
                     GGSLEPRGAYVVDAGAAAALVSGNSLLPAGVTQVEGVFERGDAVIIRTGDGAEVGRGL
                     TAYGADDASAIIGRKSSEIADILGYLGRPELIHRDDMTLKRKGS"
     gene            4182..5468
                     /locus_tag="AC16Y7_07920"
     CDS             4182..5468
                     /locus_tag="AC16Y7_07920"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020010755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR5709344.1"
                     /translation="MPLPAQDPTVSIEPMMLYMGRLAREASTELAVASTDAKNAALGR
                     MAARLDAESEKIIDANHQDLEAAEKKGRDSAFLDRLRLDPLRVAAMAKGLHEVAALPD
                     PVGATMASWTRPNGLEISRVRVPLGVIGMIYESRPNVTADASGLCLKSGNAVILRSGS
                     DSFLTSLVIANVLTEAITASGLPAASIQMVPSVDRSAVGFMLGGLDGAIDVIVPRGGR
                     SLVERVQEEARVPVFAHLEGVCHVYVDENADLDMAKEIVVNAKMRRTGICGAAETLLV
                     SHAAVSTHLTPIVDALIEAGCEVRGDPDTQRTDSRVKPATDEDWGAEYLAPIIAVKVV
                     DSLDDAIAHIAEYGSHHTDAIVTNDQDAASTCRSRVDSAIVLHNASTQFADGGEFGMG
                     AEIGIATGRFHARGPVGVEQLTTFKYVVRGQGQTRS"
     gene            5516..>5610
                     /locus_tag="AC16Y7_07925"
     CDS             5516..>5610
                     /locus_tag="AC16Y7_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinic acid mononucleotide
                     adenylyltransferase"
                     /protein_id="HIR5709345.1"
                     /translation="MRIGLLGGSFDPAHEAHREISLAALKRLGLD"
BASE COUNT         1077 a         1721 c         1854 g          958 t
ORIGIN      
        1 accctcagtg ccgcccaacc ggaccgccga attcgagaac agagaattcg ctgcctgaaa
       61 gccgtggtgc tctcagaaaa ctgctctctg ctgccacttg tcggggattt cgcgggccta
      121 actcgcggat attacgggac aatcgagaaa ggcaaaaatt cttcgtaaaa acagaggact
      181 agctggcgga gagagaggga ttcgaaccct cggaacgctt gcgcgctcaa cggttttcga
      241 gaccgccccg ttcaaccact ccggcacctc tccgaggtgg gtcatgtagc ttgccgggag
      301 gcccgccgca agccgggcaa gacgccgaaa tgccgcgtcc gccgggcttt tttgaggggg
      361 tccggcaatt gacttatggg ccttcgcctc tatattgcgc cgcttatcgc tcctttagag
      421 gcgtgccggc cccacgaaat tgtgctggca gatcaagccc caagagacgg attggaaaag
      481 atgttcgcgg tcattcgcac gggcggcaag caatatcgcg tggcgccgga agacattatc
      541 gagattgaga agatcgccgg ccagcccggc gatatcgtcc agctgggcga ggttctgctg
      601 ctgggcggcg acggttcgcc caaatcgggc agcccgacca tctccggcgc atccgtggcc
      661 gccgaggtgc tggagcagaa gcgcgacgac aagatcatcg tcttcaagaa gaagcggcgc
      721 caaggctacc gccggaccaa gggccaccgc caggctctga ccgcgctccg catcaccgag
      781 attctgaccg acggcaagaa gccgtcgaag gccgccgcca agccggcccc gaaaaaggcc
      841 gagaccaagc cggctccgaa ggcagaggcg aagcccgctg ccaaggctcc ggcgaagaag
      901 gcggctccgg ccaagaaggc tgagactaag gccccggcca agaaggcccc ggccaaaaag
      961 gcagctccgg ccaagaaggc cgcgaccaag gcgccagcca agaaggcagc cgccaaaaag
     1021 gccccggcca aaaaggccgc gcctaagaag aagagctaaa ggaacaccac catggcacat
     1081 aaaaaagcag gcggctcctc gcgcaacggg cgcgactcag ctggacggcg ccttggcgtc
     1141 aaaaagtcgg gtgggcagca ggtcatcgcc ggcaacatca tcatccgcca gcgtggcacc
     1201 aagtggcacc agggtgacaa tgtcggcatg ggcaaggacc acaccctgtt cgcgctcagg
     1261 gacggtgtcg tcgaattcgc gaccaagcag aagggccggg tctacgtctc ggtgaagcca
     1321 agcgaggcgt cttaaagccc tcgtttccct tcgtgctaac cgtttgccgc gcgccattgg
     1381 gcgcgtatag ccttttgtcc cgtgcagatc aaaacgcaaa gactcatctt gcggccgccg
     1441 gcgctcgccg atgcggcgcg tatcgccatg cttgcgggcg actacgacgt tgcgtgcatg
     1501 acgggcacca ttccgcatcc ctatagcgag cagatggcgg ccgagtggct tgccggcgcg
     1561 ctgaacgggg aagagggcgt ggtgttaatg atcgagcgcg acggcacgct cgtcggctgc
     1621 acaggctacc gggccttcgg tgccgatcac gccgagctcg gctactggat cggcaagccc
     1681 tattgggggc atggctatgc gacggaggca gcccgggcgc ttatcgcgca cgcgttcgat
     1741 gccgatgggt tcgcctatct caaagcgggc tacctcgcgg ataatctgag atccgagcgg
     1801 gtcctgcaga agcttggttt cgtggccgat gacgaggaaa tgcgcgattg cgcggcacga
     1861 ggcgacaccg tccactgcac cacctaccgg ctgagccggg ccgatgcgca agccagcctt
     1921 cgtcagccat aggccggcag gctcaaggcc ggtccccata agtgcccacg gctagtgcaa
     1981 cggtaagacc tctaatgcta agcccttctg atgcagtttc ttgattccgc aaaggtcttt
     2041 gtccgctccg gcgatggcgg caacgggtgc gtgagcttcc ggcgggagaa gtttgtcgag
     2101 tacggcggcc ccaacggcgg agacggcggc aagggcggcg acgtgatcgt ggaatgcgtg
     2161 gccaatctca acacgctgct cgacttccgc taccagcagc acttcaaagc caagaagggc
     2221 ggcaatggca tgggccagaa ccgcaccggg gcgaagggcg cggacatcgt gctgaaattg
     2281 ccgccgggga cgcaaattct cgatgaggac ggcgagacgc tgatcgctga tctcactgag
     2341 cctggccagc gccttgtctt gctgacaggc ggcaatggcg gcttcggcaa cgcgcatttc
     2401 aagacgtcca ccaatcaggc gccgcgccgg gccaatccag gcctgccggg cgaggagatg
     2461 tatatctggc tgcggctcaa gctgatcggc gatgcaggca tcgttggtct gccgaacgcg
     2521 ggcaagtcga cattccttgc cgcggtcagc gccgcgaagc cgaagattgc cgattacccg
     2581 ttcacgacgc tgcatcccaa tcttggcgtg gtgcgggtgg gcggcctcga cttcgtcctc
     2641 gccgacattc ccggcctgat cgagggcgcg cacgagggcg tcggcattgg cgatcgcttt
     2701 ctcggtcacg tggagcggac cgcggtgctg ctgcatctcg tcgatgcgac ggccgacgac
     2761 cctgttgcgg cgtatgaggt cgtgcgcggc gagatcgagg cctatggcgc tgggctcgaa
     2821 gtcaagcccg gagtgttggc cttgtcgaaa gtggatgccg tggctgacga cgaactaaag
     2881 aagaaatcgc gcgcgttgaa aaaagccgcg ggcgttacgc cgcttctcgt ctccgccgtc
     2941 acgggcgaag gcgtgcagga cgtgctgcat gcgcttgcgc gcgaggtcga gacgatgcgg
     3001 gaacgcgaga cggccgacct gtcgcgcaag acgagccaag gatggcggcc atgagatccg
     3061 aatggacagg ggcgcggcgc atcgtcgtca agatcggctc tgcccttctg gtcgacagcg
     3121 ccgccggcaa actccgcacc gagtggttcg acgcctttgc cgacgacatc gcggcgctgc
     3181 gtgccgaagg caaagaagtg atcgtggtgt cttccggcgc cattgcgctg ggccgcaatg
     3241 cgctgggttt gccgaaaggt gcgctgaagc tcgacgatag tcaggcggcc gcttcggtcg
     3301 ggcagatctc gttagcgcat gcgttcgagg acaggttccg cgcgcgggga atggtagccg
     3361 cacagattct tgtcacgctt ggcgacaccg aagagcgccg ccgctatctc aacgcgcgca
     3421 gtaccatgtc gacactgctt aagctcggcg cggtgccggt cgtaaacgag aacgacaccg
     3481 tggcgacgaa tgaaatccgc tacggggaca acgaccggct ggcggcgcgc attgcctcga
     3541 tgatgagtgc ggactgcctt gtgcttttat ccgacgtgga cgggttctat accgcgccgc
     3601 ccggcatgga cgagaacgcg cggcgtctcg acgaggtgcc tgatatcacg gctgagatcg
     3661 aagccatggc cggagacgtc ggttcggaac tgtcgcgcgg cggcatggtc accaagatcg
     3721 aggcggcaaa gatcgcggtg caagccggta cgcacatggt catcgccagc ggcaaggtga
     3781 taaagcctct ccagacgctc gccaccactg gcgtttgcac ctggttcctg gcgcattccg
     3841 atcccgttac cgcgcgcaag cgctggatcg gcggctctct tgagccgcgg ggcgcctatg
     3901 tggtcgatgc aggcgccgcg gcggccttgg tctctggaaa cagtctcttg cccgcgggcg
     3961 tcacgcaggt ggagggcgtg ttcgagcgcg gcgatgccgt gatcatcagg accggcgatg
     4021 gcgctgaggt cggccgtggc ctcacggctt acggggctga cgacgccagt gccattatcg
     4081 gccgcaagag cagtgaaatc gcggacattc tcggctacct tggccgccct gagctgatcc
     4141 atcgcgatga tatgaccttg aagcgcaaag gctcttaaga catgccactg cctgcccaag
     4201 acccgactgt cagcattgag cccatgatgc tctatatggg aaggctcgcg cgcgaggcgt
     4261 cgaccgagct tgcggtcgcc tcgacggacg cgaagaacgc ggcgctcggc cgcatggccg
     4321 cgcgtcttga tgcggagtcc gagaagatca tcgacgccaa ccaccaggac ctggaagccg
     4381 ccgagaagaa gggccgcgac tctgcatttc tcgaccgctt gcggctcgac cctttgcgcg
     4441 tggcggccat ggccaagggc ctgcacgagg ttgccgcgct gcccgatccg gtaggcgcta
     4501 ccatggcctc gtggacgcgg cccaacgggc ttgagatttc ccgcgtgcgc gtgccgctcg
     4561 gcgtcattgg catgatctac gagagccggc ccaatgtgac ggccgatgcg tccggtcttt
     4621 gcttgaagtc tggcaacgca gtgattttgc gctcaggctc cgacagtttt ctgacctcgc
     4681 tcgtcatcgc caatgtgctg accgaagcga tcacggcgtc cggattgccg gccgcatcga
     4741 tccagatggt tccgtccgtc gaccgctcgg cggttggctt tatgctcggc ggtctcgacg
     4801 gcgccatcga cgtcatcgtg ccgcgcggtg gcaggagtct cgtcgagcgc gtacaagaag
     4861 aagcgcgcgt accggtgttc gcgcatttgg aaggcgtgtg tcacgtctat gtggacgaga
     4921 acgccgatct cgatatggcg aaggagattg tcgtcaatgc gaagatgcgg cgcaccggta
     4981 tttgtggcgc ggcggagaca ttgctcgttt cgcacgcagc ggtttccacg catctaacgc
     5041 ccatcgtcga tgcgctgatc gaagcgggct gcgaagtgcg cggcgatccg gacacgcagc
     5101 gcacggatag ccgcgtcaag ccggccaccg acgaggattg gggtgcggag tatctggcgc
     5161 cgatcatcgc ggtgaaagtc gtcgacagcc tcgacgacgc catcgctcat attgccgaat
     5221 acgggtcgca tcacaccgat gccatcgtta cgaacgacca agacgcggca agcacgtgtc
     5281 ggtcgcgcgt cgacagcgcc atcgtgctgc ataacgcctc gacccagttt gccgacggtg
     5341 gcgagttcgg catgggcgcg gagatcggca tcgccacggg gcgctttcat gcgcgcggac
     5401 ccgttggcgt cgagcaactc accacgttca aatatgtggt gcgcggacaa ggccagacgc
     5461 ggtcttgagc gtgagcggcc tcgcgcatct caaggcgccc gccgctgcat ccggcatgcg
     5521 gatcggtctc ctcggcggct cattcgatcc ggcacatgag gcgcaccggg agatcagtct
     5581 cgcggcgttg aagcgcctcg ggctcgatca
//
LOCUS       DBNHVU010000187         4995 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1925805, whole genome shotgun sequence.
ACCESSION   DBNHVU010000187 DBNHVU010000000
VERSION     DBNHVU010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4995)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4995)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4995
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1925805"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="AC16Y7_07930"
     CDS             complement(<1..1003)
                     /locus_tag="AC16Y7_07930"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR5709346.1"
                     /translation="MSVHLVRLLAAALVCLVLTGCGEEATLPVEAGTGPDPELPPPVQ
                     TVLPTVVLASAKGWPDGAEPKAASGLEVKAFAEGLAHPRWLYVLPNGDVLVAESSAPP
                     QPGLSRGLMNWLMKLGMRWAGAGAPSANRITLLRDGDGDGAAEIRKTFIAGLNSPIGM
                     ALVDDSFYVANTDGVVRFPYRAGATEISAKGETVAGLPAGPVNLHWVKNIVASPDGAR
                     LYAGVGSNSNLGERGMENEEGRAAIHEIDLATGTSRLFTDGLRNPVGLAFHPTSGALW
                     TTVNERDELGSDLVPDYMTTVKDGGFYGWPYSYYGTHLDPRARPQRPDLVAKAIVPDY
                     "
     gene            1270..2589
                     /locus_tag="AC16Y7_07935"
     CDS             1270..2589
                     /locus_tag="AC16Y7_07935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5709347.1"
                     /translation="MQQRNQSTKRASDGKGAGGETPEQKPSQDKGVETKSSAAGYASG
                     RILLITLAVLALAAIGYRYLIEPQGTETAQVQAGAPPAPPVTVAKPLVQTLREWRDFT
                     GQFEAKESVEIRARVNGYLESVNFTDGQLVKKGDLLFVIEPQPFEIALESAKAQLTQA
                     EAELDLAKVQLERTAQLRKNDYASQQTFDERTAQVKGAIAAVDNAKAAVNQAQLNLDY
                     TRVLAPVSGRVGRHEVSVGNLIVGGTGETTLLTTIVSLDPIWLMFNVSEGDGMTYKRL
                     IQKGELQSARSSEVQVEGQLMDETDWPLKGAIDFVDNQYDRSSGTIRVRASFPNEDLF
                     ITPGQFGRVRVPLSQEKPTILVPDASVVTDQSVKLLFTVTEDGTVVPKPVELGSIADD
                     GLRIIRSGITSDDEIIISGLLRARPGQKVTPEKGKIKPPSAVPLSQR"
     gene            2606..>4995
                     /locus_tag="AC16Y7_07940"
     CDS             2606..>4995
                     /locus_tag="AC16Y7_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5709348.1"
                     /translation="MKISHFFIDRPIFASVISILITLIGGVAYFSLPIAQYPDIAPPS
                     IQVTAQYPGASAEIVADTVATPIEQEVNGVDDMIYMTSQSTGNGSMTLTVTFKLGTNL
                     DTAQVLVQNRVAIATPRLPEEVRSLGVQVVKQSPDLMMVIHLSSPDDSRDLLYISNYA
                     TLHVRDVLSRIDGVGNVTIFGARDYSMRVWLDPDKLAVRSLTAGDVIDALRGQNVQVA
                     AGVINQPPVPSPPGAFQLNVETEGRLTTPEAFGNIIVKSKGDGRVVRVRDIARVELGA
                     ADYNRNGYLDERVAIPMGIFQRPGSNALATADVVEATMKELAKAFPKGMRYDIVYNPT
                     VFISDSIEAVITTMLEAVVLVVLVIVIFLQTWRASLIPIIAIPVSLIGTLAVLAAIGY
                     SLNNLSLFGLVLAIGIVVDDAIVVVENVERNIREGLSPREAAHRSMDEVGTALIAMTL
                     VVCAVFIPTVFLGGILGQFFRQFAVTIATATLISLVVSLTLSPALCALLFKPHEPYEQ
                     RKSIISRGIGLFFHWFNVGFEWVSFKYGALTARLLRISGVMLAVYAGLIGLTVFQFRS
                     APTGFIPNQDLGYLINIIQLPAGSSLARTDEVARQVTRVALDTPGVAHAVPIVGLDGA
                     TFTTAPNSAVVFTPLDSFKERAKNGLSAQAIAGKLNQGFAGIRDAFVLSISPPPVRGI
                     GTTGGFKMMAQDTKGSPLEQLEEVSQSIVGAANQQPGLSRVFTTFNTRTPKVYADIDR
                     VRAEMLGVTADDVFQALEVYLGSQYVNDFNFLGRTYRVTAQADGQFRQDLHAISQL"
BASE COUNT          914 a         1608 c         1543 g          930 t
ORIGIN      
        1 catagtccgg cacgatggcc ttggcgacga ggtccggccg ctgtggccgc gcgcgcggat
       61 ccagatgcgt gccgtagtag ctatacggcc agccatagaa gccgccgtcc ttcaccgtgg
      121 tcatgtaatc cggcacgagg tcgctgccga gttcgtcccg ttcgttgacc gtggtccaca
      181 gcgccccgct cgtcggatga aacgcgagcc cgaccgggtt gcgcaggccg tcggtgaaga
      241 ggcgggaagt gccggtcgcc aagtcgatct catggatcgc ggcgcggcct tcttcgttct
      301 ccatgccacg ctcgcccaga ttgctgttgg agccgacgcc ggcgtagagg cgtgcgccgt
      361 caggactggc gacgatgttc ttcacccaat ggagattgac cgggccggca ggaaggccgg
      421 cgaccgtctc gcccttcgcg ctgatctcgg ttgcgcccgc gcgatacgga aaccggacaa
      481 cgccgtccgt gttggccacg tagaagctgt catccaccag agccatgccg atgggtgagt
      541 tcaagccggc aataaacgtc ttgcggatct cggccgcgcc gtcgccgtcg ccatcacgca
      601 acagcgtgat gcgatttgcg ctcggggcgc cggcgccggc ccaccgcatg cccagcttca
      661 tgagccagtt catgagcccg cgcgagaggc cgggctgggg cggcgcggaa ctctctgcca
      721 ccagcacgtc gccgttggga agaacataca gccagcgcgg gtgagcgagc ccctcggcga
      781 aggccttgac ctccaaacct gatgccgctt tgggctcggc gccgtccggc cagcctttcg
      841 cggatgccaa cacaaccgtc ggcaggacgg tttgcaccgg tggcggaagt tcagggtcag
      901 gtccggtccc cgcctcgacc gggagcgttg cctcctcgcc gcaaccggtc aggacaaggc
      961 aaaccaaggc ggcggcaaga agccttacaa ggtggacgga cataagaagg gcctcggggt
     1021 tgtgttgcgt tcgcactata gcgcgacatt ggcggcggcc acgagatgtc tcctgctcag
     1081 gacgcgggac aaaatggcgt gatcgccgcc ctgacatggc cggaagggcg gtatcgtgct
     1141 cgaggcttgc gtatcggtgc gctaatgccc tctgggtttg attggctgtt tggggtagcg
     1201 ttcctgtcga gacctgatgc cgttgcccca accctcgcgc tcggcagcct caccccttga
     1261 gaagccaaga tgcagcaaag aaaccaaagc acgaagcgcg cgagcgacgg caagggcgcc
     1321 gggggcgaga cgcccgaaca gaagccctcc caagacaagg gcgtggagac caagagctcg
     1381 gcggcgggat atgccagcgg ccgcatctta ttgatcaccc tcgcggtcct tgcgcttgcg
     1441 gcgatcggct atcgctactt gatcgagccc caagggaccg agacggccca agtacaggcc
     1501 ggagcaccgc ccgcaccgcc cgtcaccgtg gccaagccgc tggtgcagac cttacgtgag
     1561 tggcgcgatt tcaccggcca attcgaggcc aaggaatctg tcgagatacg tgctcgggtg
     1621 aacggctatc tggaatccgt caacttcacc gacggtcagc tcgtgaagaa gggcgacctt
     1681 ctctttgtca tcgagccgca gcccttcgag atcgcgcttg agtccgccaa ggcgcagctc
     1741 acgcaagccg aggccgaact cgatctcgcc aaggtgcagc tcgagcgtac cgcgcagcta
     1801 cgcaagaacg actatgcctc gcagcagaca ttcgacgaac ggaccgcgca ggtgaaaggt
     1861 gccatcgccg ccgtcgacaa cgccaaagcc gcagtcaacc aggcgcagct caatctcgac
     1921 tatacgcgcg tgctggcgcc ggtttcgggc cgggttggcc ggcacgaggt cagcgtgggc
     1981 aatctcatcg tcggcggcac gggcgagacc acgctgctga ccaccattgt ctcgctggat
     2041 ccgatttggc tgatgttcaa tgtcagcgag ggcgacggca tgacctataa gcgcctcatt
     2101 cagaaggggg agctccagtc cgcccgcagc agtgaagtgc aagtcgaagg ccagctcatg
     2161 gacgagaccg attggccgct gaaaggcgcg atcgatttcg tcgacaatca gtacgaccgc
     2221 tcgtccggca cgatccgcgt tcgcgcctcc ttccctaacg aagacctgtt tatcacgccc
     2281 ggtcagttcg gccgtgtgcg cgtgcccttg tcgcaagaaa agccgacgat cttggtgccc
     2341 gacgcgtcgg tggtcacgga ccagtcggtc aagctactct ttacggtcac cgaagatggc
     2401 acggtcgtgc cgaagcctgt cgaactcggt tcgatcgccg atgacggctt gcgcattatc
     2461 cgaagcggca tcacgtccga tgacgagatc atcatcagcg ggctcttgcg cgcgcgcccc
     2521 ggccagaagg tcacgcccga gaaaggcaag atcaagccgc cttcggccgt gccactgagt
     2581 cagcgatgac ggatcggccc cggcaatgaa gatctctcac ttctttatcg accggccaat
     2641 tttcgcatcg gtcatctcga ttctgatcac gctgatcggc ggcgtggcct atttcagcct
     2701 gccgatcgcg cagtatccgg acatcgcccc gccgtcgatc caggtgacgg cgcagtatcc
     2761 aggcgcgtcc gccgagatcg tcgccgacac ggtcgccacg ccgatcgagc aggaagtgaa
     2821 cggcgtggac gacatgatct acatgacgtc gcagtcgacc ggtaacgggt ccatgaccct
     2881 gaccgtgacg ttcaagctcg gcacgaatct cgatacggcg caggtgctgg tgcagaatcg
     2941 cgtggccatc gccacgccgc gcctgccgga ggaggtccgc tcgctcggtg ttcaggtcgt
     3001 caagcagtcg cccgatctga tgatggtgat tcatctgagt tcgcccgacg acagccgcga
     3061 cctgctctac atctccaact atgctacgct gcacgtgcgg gacgtgctct cgcgcatcga
     3121 cggcgtcggt aacgtgacga tcttcggcgc gcgcgactac tccatgcgcg tctggctcga
     3181 tcccgataag cttgcggttc gaagcctcac ggccggcgac gtgatcgatg cgctgcgggg
     3241 gcagaacgtg caagtcgccg ccggcgttat caaccagccg ccggtgccgt ctccgcccgg
     3301 cgcgttccag ctcaacgtcg aaacggaagg ccgtctcacc acaccggagg ccttcggcaa
     3361 catcatcgtc aagtccaaag gggatggccg cgtggtgcgc gtgcgcgaca tcgcccgcgt
     3421 cgaactcggc gccgcggact ataaccgcaa cggctatctc gacgagcggg tcgccatccc
     3481 gatgggtatc ttccagcggc ccggttcgaa cgcgctcgcg accgccgacg tggtcgaggc
     3541 gacgatgaag gagcttgcca aggccttccc caagggcatg cgctacgaca tcgtctataa
     3601 cccgaccgtc tttatctccg attcgatcga ggcagtgatc acgaccatgc tcgaggcggt
     3661 cgttctcgtc gtcctcgtga tcgtcatatt cctgcagaca tggcgtgcgt cgctcatccc
     3721 aattatcgcg atcccggtat cgttgatcgg cacgctcgcc gtgctggccg cgatcggtta
     3781 ttcactgaac aacctttcac tcttcgggct cgtgctcgcg atcggcattg tcgtcgacga
     3841 cgcgatcgtc gttgttgaga atgtcgagcg gaacattcgt gagggtcttt cgccgcgcga
     3901 ggccgcgcat cgcagtatgg acgaggtggg aacggcgctg atcgccatga cgctcgtagt
     3961 ctgcgcagtg ttcattccca ctgtcttcct aggcggcatc ctcgggcagt tcttccggca
     4021 gtttgccgta acgatcgcca cggctacgct gatctcgctt gtcgtctcgc tcaccttaag
     4081 cccagcgctt tgcgccctcc tgttcaagcc gcacgagccg tacgagcagc gtaagagcat
     4141 aatttcgcgc gggattggcc ttttcttcca ttggttcaat gttggcttcg aatgggtgtc
     4201 cttcaaatac ggtgcgctca ccgcacggct gctgcgcatt tccggcgtga tgctcgcggt
     4261 ctatgccggt ctaatcggac tgacagtctt ccagttccgc tccgcaccca ccggcttcat
     4321 cccgaaccag gacctcggct atctgatcaa catcatccaa ttgcccgcgg gttcatcgtt
     4381 ggcgcggacg gatgaggtgg ctaggcaggt tacgcgggtt gcactggata cgccgggcgt
     4441 ggcgcacgcg gtgcccattg tcggtttgga cggcgccacg ttcaccacgg cgccaaactc
     4501 cgcggtcgtg ttcacgccgc tcgattcgtt caaggagcgc gccaaaaacg gactgagcgc
     4561 gcaagcgatc gctggcaagc tgaatcaagg cttcgcaggc attcgagatg ctttcgtcct
     4621 cagcatttcg ccgccgcccg ttcgcggcat cggcacaacc ggcggattca agatgatggc
     4681 gcaggacacc aagggatccc cgctcgagca gttggaggag gtctcgcaaa gcatcgtggg
     4741 tgccgccaac cagcaacccg gacttagccg cgtcttcacg accttcaata cccgcacgcc
     4801 gaaggtctat gcggacatcg atcgggtcag ggccgagatg ctgggcgtca ccgcggacga
     4861 tgtgtttcag gctctcgagg tctatctcgg ctcgcaatac gtgaacgact tcaatttctt
     4921 ggggcgcacc taccgcgtga cggcgcaggc cgacggccag ttccggcagg atctgcacgc
     4981 catctcccag ctcca
//
LOCUS       DBNHVU010000188        11311 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_736125, whole genome shotgun sequence.
ACCESSION   DBNHVU010000188 DBNHVU010000000
VERSION     DBNHVU010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 11311)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11311)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11311
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_736125"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2098
                     /locus_tag="AC16Y7_07945"
     CDS             <1..2098
                     /locus_tag="AC16Y7_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR5709349.1"
                     /translation="ARPGQILAVTFTNRAAREMKTRVAELIGGVVEGMPWLGTFHAIG
                     VKILRRHAELVDLKSSFTVLDTDDQIRLLKQLLAAENIDEKRWPARLLAAMIDGWKNR
                     GLTPARVPDGEAFGFANGKGRDLYALYQRRLKELNAADFGDLLLENLRLFQEHPDVLG
                     KFQNRFTYMLVDEYQDSNVAQYLWLRLLAQGTSNICCVGDDDQSIYGWRGAEVDNILR
                     FETDFPGAKVIRLERNYRSTGHILAAASGLIAHNQGRLGKTLHTEGDEGEKVVVEGCW
                     DDEEEARVIGEDIEQLQRDGHALNEFAVLVRASFQMRAFEDRFITLGLDYRVIGGPRF
                     YERQEIRDATAYFEVTLNPSNDLKFERIINTPRRGLGDATLKALHQLARANGVPLTQA
                     ARLIVETEELRPKPRKALADLLASFDRWRSLVDTMRHTELAELILDESGYTAMWQADR
                     SQQAQGRLENLKELIRFMEEFDTLAGFMEHVSLVMDAATEEGTDRVNLMTLHSAKGLE
                     FDTVFLPGWDEGLLPHQRSLDESGRSGLEEERRLAHVGLTRARKRAKITFAQNRRNHG
                     MWQTAVPSRFIDELPVDHVEVAEDKGLGGYGPSRFENAETLFASGAYETPGWQRAQRN
                     RQGGSSGPRREPLTIEGSLVAASTAEGSGVDVGERIFHQKFGYGRVASIDGNKLTVDF
                     EKAGRKKVVDTFVERV"
     gene            2085..2639
                     /locus_tag="AC16Y7_07950"
     CDS             2085..2639
                     /locus_tag="AC16Y7_07950"
                     /EC_number="4.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF016627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HIR5709350.1"
                     /translation="MSGCDANPMWLFGYGSLMWDGWETAYTCARRMPADLPGHARVFN
                     KKSLERWGTHARPGLTLNLARTGACRGMAFAFDDGARSDIEAYLNHRETCEATEVAVR
                     LPDGTEVGALTYIYGGPRLIDEDLSLHARASMIVEASGIAGSSYAYIKGVREHLTELG
                     VTDAAVDELWQAVATLKDGARDRE"
     gene            2636..3538
                     /locus_tag="AC16Y7_07955"
     CDS             2636..3538
                     /locus_tag="AC16Y7_07955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018076.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR5709351.1"
                     /translation="MTKASLTYRATVEAAPLAAVRIAEVLDEVDDPSALSVSFFELGT
                     GTYEVSALYGNRPDKTALEALIEAAADSEPVVPLRVEELPPTDWVTLSQGQRGPVRAG
                     RFLVHGSHDRDAVVRNLFTIEIDADQAFGTAHHASTRGCLLMLDALAKRGHPDLVLDL
                     GTGTGILAIAAALAFDRPVVATDNDPVAVRIANDNAEKAGVAQQVHALQADGLAHPKL
                     QRLSPDLIIANILAGPLYDLAPGLAQTLQPGGFIILSGLTAEQAPGIQARYGAHGFVL
                     EKRIILDGWATLLLGRRSARILSD"
     gene            3572..5392
                     /locus_tag="AC16Y7_07960"
     CDS             3572..5392
                     /locus_tag="AC16Y7_07960"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HIR5709352.1"
                     /translation="MYQSFDEASAPAASSDRVQALLRELKSRRLKGFLVPHSDQHQNE
                     FLPPADERLAWLTGFAGSAGIAIVTDKGSALFVDGRYILQAPEQVDTSLFEVLQVPQA
                     KPSSWIKETLVRGNAVGYDPALHTIKEIDRLTKTLGAAGIRLNPVADNPIDKLWGSDR
                     PKPPSAPIYLQPLEFAGRSPAEKIAQVQKTLAEDEQDAVLLTAPDSICWLFNFRGGDI
                     KHTPVALTFAIVPASGRPTLFIDPGKVGDNVHGALSGFIDIAESEALEEKLKALAEGG
                     AKVRLDPDQVSMRFARILEENGTKFVAGDDPCTLPKAIKTRAEIEGARAAHLRDGVAM
                     ARFLAWLDEVSESGREDEVSAAMKVEDFRRQSGQLKDISFDTIAASGPHGALVHYRPL
                     NATKRVLDKNSLFLIDSGGQYVDGTTDITRTVAVGKPTAEMKRHYGLVLKAYIAVASA
                     RFPKGTRGQDIDPYARRPLWEAGLDFDHGTGHGVGSYLSVHEGPQRLSRLGTEELKPG
                     MILSNEPGYYRKGKYGIRLENLILVTPLEKIEGGEREMMGFETLTLVPFDRRLIDPKQ
                     FEPSELAWLNDYHARVRREIEPLVLSDDRPWLRHATAKIG"
     gene            complement(5393..7504)
                     /gene="ligA"
                     /locus_tag="AC16Y7_07965"
     CDS             complement(5393..7504)
                     /gene="ligA"
                     /locus_tag="AC16Y7_07965"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR5709353.1"
                     /translation="MSKKETKPVAKLDEAEATQELARLAKEIAHHDALYYRKDAPEVS
                     DAAYDALRQRNEAIEARFPHLVRDDSPSLKIGAAPVEAFGKVTHRVPMLSLGNVFDDD
                     GVRDFLERVRRFLGLDAEAELAFTTEPKIDGLSITLRYEGGRLVQGATRGDGYEGENV
                     TANVRTIEDIPKEVDARGFPDPFEVRGEIYMSHSDFQALNAAQEKAGNRPFANPRNAA
                     AGSLRQLDPAVTALRPLRFFAYSWGEVAALPADTQWGVYQAMADWGFPLNPLAKLTSS
                     VDEMLDTYREIEEGRAGLDYDIDGIVYKLDRLDLQERLGFVSRSPRFAIAHKFPAEKA
                     TTILRDIEIQVGRTGSLTPVAKLEPVTVGGVVVQNATLHNEDEIERKDVRIGDTVVLQ
                     RAGDVIPQILGPVREKRPRGAKRFKFPDVCPACGSHAVREVDERTGKQDVVRRCTGGL
                     ICPAQRVERLKHFVSRNAFDIEGLGEKNIEAFYDEGLIQSPPDIFTFQARDRDAKEKL
                     SEREGWGETSAQKLFDAIEARRDVRLDRFIYALGIRHVGEITGRLLARTYGTAEDFFK
                     AMRAAAKARDSEAFAELENIEGIGPTVAAAIADFFGEPHNVDVVEALLKEVSPEALEA
                     VDHASPVSGKTVVFTGTLEQMTRAEAKARAERLGAKVAGSVSKKTDYVVAGPGAGSKL
                     KNAKALGVAVLTEDEWLKLIG"
     gene            complement(7517..9199)
                     /gene="recN"
                     /locus_tag="AC16Y7_07970"
     CDS             complement(7517..9199)
                     /gene="recN"
                     /locus_tag="AC16Y7_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR5709354.1"
                     /translation="MLAALSIRDIVLIDKLDLEFGAGLSALTGETGAGKSILLDAFSL
                     AIGARGDASLVRRGVEQGQVTATFELAADHPVLALLAENGIAIEDDVLILRRVQGNDG
                     RSRAFINDTGVSVQLLRQVGRALVEIHGQHDERALIDPSGHRDLIDAFGGLGADAAKL
                     AELYEAWGEADAARARHESEIAATRANAEYVAHALEELQTLAPEADEEEALASRRQLM
                     MNAEKIAGDLNEAMDALQGEGTAGARLASALRRLERQADSGGPVLTGVAETLERVLNE
                     TNAARDKIEEALALTAFEPADLERAEERLFALRAVARKHRVRVDDLPALTERLQAELE
                     ALDTSEGRATELAEAAAQARKAYEQAALALSKARAKAAKRLDQEVAAELAPLKLEKAR
                     FVTHIDAVDLAEGGPSGVDRVAFWVATNPGTDPGPMMKVASGGELARFILALKVVLAA
                     RGSAPTLIFDEADSGVGGATAAAVGERLSGLADSVQVLAVTHAPQVAALAAGHLMIAK
                     ETVAGPKGEAMATRVAVLEGEHRREEIARMLAGQTITDEARAAADRLMRKSA"
     gene            complement(9210..10085)
                     /locus_tag="AC16Y7_07975"
     CDS             complement(9210..10085)
                     /locus_tag="AC16Y7_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR5709355.1"
                     /translation="MVSPEEPRRAFAPRVARWCGLGTCLAAGLLAGCGSSIFGSGDDS
                     AEIVSASPPNAIYGDAETLINEGDYQEAARKYEQVDINHPYSPEARQAIVMSAYAYYK
                     AGKYDEAIGAADRYLTLHPGTQESAMAQNIIGMSYYDRVLDPKRDQTFARRGLAAYDK
                     LLQRYPNSRYSGEASNRARILRDLLAANEMTVGRYYLRRNNFLAAINRFRTVVTSYQT
                     TEQVEEALMRLTEAYMALGIVNEAQTAAAVLGHNFPDSKWYKHAYRLLGKRGLEPQQY
                     EGSWITRVWNGGGEA"
     gene            complement(10287..11237)
                     /gene="lpxC"
                     /locus_tag="AC16Y7_07980"
     CDS             complement(10287..11237)
                     /gene="lpxC"
                     /locus_tag="AC16Y7_07980"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103117 - UDP-3-O-acyl-N-acetylglucosamine
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HIR5709356.1"
                     /translation="MKGFIGARQTTLACEISLTGTGVHSGAPVSISICPADGDTGLRF
                     LISNGDSDGIEIAADQQWVTGVTLCTELGDGNGASVATVEHVLAALRGLGVDNALIEI
                     DSVEVPIMDGSAAPFVEAIDEVGLTELDAPRRFLKVLKPICVEDGGAVGELVPHNGFH
                     LDVEIDYETKLIGKQKLEMDVNPGSFRREISRARTFGFMKDVERLWAAGLALGASLEN
                     TVAIGDDRIINREGLRFADECVRHKALDAVGDLALAGAPILGAYRSRHGGHRLNALVL
                     KALFADADAWTMVEAPRYREVRHAELSQRLAVANFAADRS"
BASE COUNT         1988 a         3730 c         3599 g         1994 t
ORIGIN      
        1 cgcccggccc ggccagatcc tcgcggtgac cttcaccaac cgggcggcgc gcgagatgaa
       61 gacacgggtc gccgaattga taggcggcgt ggtcgaaggc atgccgtggc tggggacgtt
      121 ccacgccatt ggggtgaaga ttttgcgtcg acacgcggag ctggtcgatc tcaagtcgtc
      181 cttcacggtg ctcgatacag acgatcaaat ccggctgctc aagcaattgc tcgcggcgga
      241 gaatatcgac gagaaacgtt ggccggcgcg actgctcgcc gccatgatcg acggctggaa
      301 gaaccgcggg ctcacacctg cccgcgtgcc ggacggcgaa gctttcggtt tcgccaacgg
      361 taaggggcgc gatctctacg cgctctatca gcgccggctc aaagagctga acgccgccga
      421 ctttggcgat ctcttgctgg agaacttgcg gctgttccag gagcacccgg acgtgcttgg
      481 gaaattccag aatcgcttca cctacatgct ggtggacgag tatcaggact ccaacgtcgc
      541 gcaatatctg tggctgcgcc tcctggcgca gggcaccagt aatatttgct gcgtcggtga
      601 cgacgaccag tccatctatg gctggcgcgg cgcggaggtg gacaacatcc tgcgcttcga
      661 gaccgacttc cccggcgcca aggtgatccg gctcgaacgc aattaccgct cgacgggtca
      721 tatcctcgcg gctgcctccg gcctcatcgc ccacaaccaa gggcggctcg gcaagacgct
      781 gcacaccgaa ggcgacgaag gcgagaaggt cgtggtcgag ggctgctggg acgacgagga
      841 agaggcccgc gtcatcggcg aggacatcga gcagctgcag cgcgacggtc atgcgctcaa
      901 cgagttcgcc gttctcgtgc gcgcgtcctt ccagatgcgc gccttcgagg accgctttat
      961 cacgctcggc ctcgactacc gcgtgatcgg cggcccgcgc ttctacgagc gccaggaaat
     1021 ccgcgacgcg acggcctatt ttgaagtgac gctgaacccg tcgaacgatc tgaagttcga
     1081 gcgcatcatc aatacgccac gccggggctt gggcgacgcc acgttgaagg cgctgcacca
     1141 actcgcccga gcaaacggcg ttcccctgac gcaagcagcc aggctgatcg tcgagaccga
     1201 ggaactgagg ccgaagccgc gcaaggcgct tgccgatctc cttgcgtcct tcgaccgctg
     1261 gcgctcgctc gtcgacacga tgcgccacac ggagctcgcc gagctgatcc tcgatgaatc
     1321 cggctacacc gccatgtggc aggcggaccg ctcccaacag gcgcaagggc gcttggagaa
     1381 cctgaaggag ctgatccgct tcatggagga gttcgacacg ctcgcgggct tcatggagca
     1441 tgtctcgctc gtcatggacg cggcgaccga agaaggcacc gaccgggtca acctgatgac
     1501 gcttcattcg gccaaggggc tggaattcga caccgtgttc ctgcccggct gggacgaagg
     1561 gctcctgccc catcagcgca gcctcgatga gtccggacgg tcgggcctcg aagaagagcg
     1621 gcggctggcc catgtggggc tgacgcgcgc gcgcaagcgc gccaagatca ccttcgcgca
     1681 gaaccggcgc aatcacggca tgtggcaaac ggcggtgccg tcgcgcttca ttgatgagtt
     1741 gcccgtcgac catgtggagg tcgccgagga caagggcctg ggcggttacg ggccgagccg
     1801 gttcgaaaac gccgagaccc tgttcgcgag cggcgcctac gagacgccgg gctggcagcg
     1861 cgcccaacgc aatcgccaag gcgggagttc ggggccccgg cgcgagccgc tcaccatcga
     1921 aggctcactg gtcgccgcca gtacggcgga aggctcaggt gtcgatgtcg gcgaacgcat
     1981 cttccaccag aagttcggct atggccgcgt cgcctcgatc gacggcaaca agctcaccgt
     2041 cgatttcgag aaggctggac gcaagaaggt ggtcgacacc ttcgttgagc gggtgtgacg
     2101 cgaaccccat gtggctgttc ggctatggct cgttgatgtg ggacggttgg gagacggcgt
     2161 atacttgcgc gcggcgcatg cccgccgacc tccccgggca cgcccgcgtc ttcaataaga
     2221 agtctctgga gcgttgggga acacatgcgc ggccaggatt gacacttaat ctggcgcgga
     2281 ccggcgcctg ccggggcatg gccttcgcct ttgacgatgg cgcgcgcagt gacatcgagg
     2341 cttatctcaa ccatcgggag acctgcgagg cgacggaagt tgccgtgagg ctgccggacg
     2401 gcaccgaggt tggggcactc acctatatct atggcggccc ccggctgatc gacgaggatc
     2461 tctcattgca cgcgcgcgcg tcgatgatcg tggaggcctc aggcattgcc ggatcgagct
     2521 acgcgtacat caagggcgtg cgcgaacatc tcaccgaact cggcgtcacc gatgcggcgg
     2581 tggatgagct gtggcaggcc gtggccacat taaaggatgg agcgcgagac cgagaatgac
     2641 gaaagcctcc ctcacctatc gcgccactgt ggaagcggcg ccactggccg cagtccggat
     2701 cgcggaagtc ctcgatgaag tcgacgatcc gtccgccctc tcggtcagct tcttcgaact
     2761 cggcacgggc acttacgaag tctcggcgct ttatgggaac cgcccggaca agaccgcgct
     2821 cgaggcattg atcgaggcgg ccgctgatag cgagcccgtc gtgccgcttc gcgtcgaaga
     2881 gcttccgccg acggactggg tgacactcag ccaaggacag cgcggtccgg tgcgcgccgg
     2941 gcgttttctg gtccatggca gccatgatcg ggacgcggtt gtacgcaacc tcttcacgat
     3001 cgagatcgat gccgaccaag ccttcggcac ggcgcatcat gcgagcacgc ggggctgtct
     3061 tctgatgctc gacgcgctcg ccaagcgcgg gcaccccgac ctcgtgctcg accttggcac
     3121 agggaccggc attttggcga tcgccgccgc actcgccttc gaccggcccg tcgtggcgac
     3181 cgacaacgat cccgtggccg tcaggatcgc caacgacaat gcggagaagg ccggggtcgc
     3241 gcagcaggtg catgcgcttc aggccgacgg cctcgcccac cccaagctgc agcgcctctc
     3301 cccggacctc atcatcgcca atatcctggc cgggccgctt tacgatttgg cgccggggct
     3361 ggcccagacc cttcagccgg gcggattcat catcctgtcc gggctgacag cagagcaggc
     3421 acccggcatc caggcacgct atggcgccca tggcttcgtc ctggaaaagc gaatcattct
     3481 cgatggatgg gcgaccctgc tgctcgggcg tcggagcgca aggattttgt ctgattgatc
     3541 gggaccgacg cttcggctta caaatccggc catgtatcag agtttcgatg aggccagcgc
     3601 tccggcggca agcagcgacc gggtccaggc attgttgcga gagctgaagt cccggcgact
     3661 gaagggcttc ctcgttcccc atagcgatca gcaccagaac gaatttctgc ctcctgccga
     3721 cgagcgcctg gcttggctga ccgggttcgc cgggtccgcc ggcatcgcca ttgtcaccga
     3781 caagggctcg gcgctgttcg tggacggccg ctacattctt caagcgccgg agcaggtcga
     3841 caccagcctc ttcgaggtgt tgcaggtccc gcaagccaaa ccgtcgagtt ggatcaagga
     3901 gacgctggtg cgcggcaacg cggtgggcta cgacccggcg ctgcacacga tcaaggagat
     3961 cgatcggctt accaagacgc ttggtgccgc cggcatccgg ctcaacccgg tcgcggataa
     4021 tccgatcgac aagctttggg gctcggaccg gccgaaaccg ccgagcgcac cgatctatct
     4081 tcagccgctc gaattcgccg gccgcagtcc ggccgaaaag attgcgcaag tgcagaagac
     4141 gctcgccgaa gacgagcagg acgcggtgct gctgacggcg ccagactcca tctgctggct
     4201 gttcaatttc cgtggcggcg acatcaaaca cacgcccgtg gcgcttacgt tcgccattgt
     4261 gcccgcgtcc ggccgcccca ccctgttcat cgatccgggc aaggtcggcg acaacgtcca
     4321 tggtgcgctc tcgggcttca tcgacattgc cgagtcggag gccctggagg aaaagctgaa
     4381 ggcgctggcc gagggaggcg cgaaggtccg gctcgatccg gatcaggtgt ccatgcgttt
     4441 cgcccgcatc ctggaagaga acggcacgaa gttcgttgcc ggcgacgatc cgtgcacgct
     4501 gccaaaggcc atcaagacga gggccgagat cgagggtgca cgcgcggcac atttgcgcga
     4561 cggcgtggcc atggcgcgct ttctcgcttg gctcgacgag gtgtctgagt ccgggcgcga
     4621 ggacgaagtc tcggcggcca tgaaggtcga ggactttcgc cgtcaatcag gccagctcaa
     4681 ggacatcagc ttcgacacca tcgcggcctc agggccgcat ggcgccttgg tgcattatcg
     4741 cccgttgaac gccaccaagc gcgtgctcga caagaactcg ctgtttttga tcgactccgg
     4801 cggccagtat gtggacggca ccacggacat cacgcgtacg gtcgcggtgg gcaaacccac
     4861 ggccgagatg aagcgccact acgggctggt gctgaaggca tacatcgccg ttgcgtcggc
     4921 gcgcttcccc aagggcaccc gcggccagga tatcgatccg tatgcgcggc gccccttatg
     4981 ggaggccggc ctcgacttcg accacggcac ggggcatggc gtcggcagct atctgtcggt
     5041 gcatgaaggc ccgcagcgcc tgtcccggct tggcaccgag gagctgaagc ccggcatgat
     5101 cctgtccaac gagcccggct attaccgaaa aggcaaatac ggcatccggc tggagaacct
     5161 gatactggtc acgccgctgg agaagatcga aggcggggag cgcgaaatga tgggcttcga
     5221 gacgctgacc ctcgtgccgt tcgaccggcg actcatcgac ccgaaacagt tcgagccctc
     5281 ggagctcgcc tggctgaacg actatcacgc tcgggtgcgc cgagagatcg agccgctggt
     5341 cctcagcgac gaccgaccct ggctgcgcca tgccacggcg aagatcggct agctagccga
     5401 tcagcttgag ccattcgtct tccgtcagca ccgcgacgcc taacgctttg gcatttttga
     5461 gcttcgagcc ggcgccgggg cccgccacca catagtcggt cttcttggaa accgagcccg
     5521 ccaccttggc gccgagacgt tcggcacggg ccttggcctc ggcccgcgtc atctgctcca
     5581 gcgtgccggt gaacaccacg gtcttgcccg aaaccggcga ggcgtggtcg accgcctcta
     5641 gggcttccgg gctcacctct ttcagcaagg cctccaccac atccacgttg tgcggctcgc
     5701 cgaagaagtc ggcgatggcc gcggccacgg tcgggccgat gccttcgatg ttctcaagct
     5761 cggcgaaggc ttcactgtca cgagccttgg cggcggcgcg catggccttg aagaaatcct
     5821 ccgcagtgcc gtaggtgcgc gccagaagac gcccggtgat ctcgccaaca tggcgaatgc
     5881 caagcgcgta gatgaagcga tcgagacgca catcgcgccg ggcctcgatg gcgtcaaaca
     5941 gtttctgtgc cgaagtctca ccccagccct cgcgctcgct gagtttctcc ttcgcgtccc
     6001 tatcgcgtgc ctgaaacgtg aagatgtccg gcggcgattg gatcaaccct tcgtcgtaga
     6061 acgcctcgat gttcttctcg ccgagccctt cgatgtcgaa ggcgttgcgc gagacgaaat
     6121 gcttcagccg ctccacacgc tgggccggac aaatgagccc gcccgtgcag cggcgcacca
     6181 cgtcctgctt gccggtgcgc tcgtcgacct cgcgcaccgc atgactgccg caggccgggc
     6241 acacatcggg aaacttgaat cgtttcgcgc ccctagggcg tttttctcgc acaggcccaa
     6301 gaatctgcgg gatgacgtcg ccggcgcgct gcaacaccac cgtgtcgcca atgcgcacgt
     6361 ccttgcgctc gatctcatcc tcgttgtgga gcgtcgcgtt ctgcaccacg acgcccccca
     6421 ctgtgaccgg ctccagcttg gcgacggggg tcagcgagcc ggtccgccct acctggatct
     6481 cgatgtcgcg caagatcgtc gtcgccttct cggccgggaa tttgtgcgcg atggcaaagc
     6541 gcggcgagcg ggagacgaat ccgagccgct cctgaagatc gagccggtcg agcttgtaga
     6601 caatgccgtc gatgtcatag tcgagccccg cccttccctc ctcgatctcg cgataggtgt
     6661 ccagcatctc gtccacggag ctggtgagct tcgccagcgg gttcagcgga aagccccaat
     6721 ccgccatggc ctgatagacg ccccattggg tgtccgcggg gagcgcggcg acctcccccc
     6781 agctataggc gaagaagcgc aagggccgca aagccgtgac ggcggggtcg agctgtcgga
     6841 gcgagcctgc cgccgcgttg cgcggattgg cgaacggccg gttgccggcc ttctcttgcg
     6901 cggcgttgag cgcctgaaag tcgctatggc tcatatagat ctcgccgcgc acctcgaagg
     6961 gatcgggaaa cccgcgtgcg tccacctctt tcggaatgtc ctcgatggtg cggacattgg
     7021 cggtgacgtt ctcgccctca tagccgtcgc cgcgcgtcgc cccttgcacc aggcggccgc
     7081 cctcgtagcg gagcgtgatg gagagaccgt cgatcttcgg ctcggtggtg aaggcaagct
     7141 ccgcctccgc gtccagccca aggaagcgcc gcacgcgttc gagaaaatcg cgcacgccgt
     7201 cgtcgtcgaa tacattgccg agcgagagca tgggcacacg atgggtgacc ttgccgaagg
     7261 cctcgaccgg cgcggcgcca atcttcagtg atggactgtc atcacggaca aggtgcggaa
     7321 agcgcgcctc gatcgcctcg ttgcgctggc ggagcgcatc gtacgcggcg tcggaaacct
     7381 ccggcgcgtc cttgcggtaa tacagcgcgt cgtgatgagc aatttctttg gcgagccgcg
     7441 ccagctcctg cgtggcttcg gcctcgtcga gcttcgccac gggctttgtc tctttcttgc
     7501 tcatgctttc agagtcttat gccgacttgc gcataagccg gtcggcggcc gcgcgggctt
     7561 cgtcggtaat ggtttggccg gccagcatac gggcgatctc ctcgcggcga tgctcgccct
     7621 caagcaccgc gacccgcgtg gccatggcct cgcccttggg ccctgcgacc gtttccttgg
     7681 caatcatcaa gtgcccagcc gcgagcgcgg cgacttgcgg ggcgtgggtc acggccagca
     7741 cctggacact gtccgcaagt cccgacaggc gttcgccgac agccgcggcc gtagcgccgc
     7801 cgacaccgga atccgcttcg tcgaaaatga gtgtcggcgc cgacccgcgc gcggcgagca
     7861 ccacttttag ggccagaatg aaacgcgcca gctcgccgcc cgaagcgacc ttcatcatcg
     7921 gaccgggatc ggtgcctgga ttggtggcga cccagaaggc gacgcggtcg acgccgctgg
     7981 ggccgccttc ggcgaggtcc acagcatcaa tgtgggtgac gaagcgcgcc ttctccagtt
     8041 tgagtggcgc aagttccgca gcgacctctt ggtccagccg cttggccgcc ttcgcgcgcg
     8101 ccttgcttaa agcgagtgcc gcttgttcgt aggctttccg cgcttgcgcg gcggcttcgg
     8161 caagctcagt cgcgcggccc tcgctggtgt ccaaggcttc gagttctgct tgaaggcgtt
     8221 cggtgagcgc cggcagatcg tccacccgaa cgcgatgctt gcgggccacg gcgcgcaagg
     8281 caaacagccg ctcctcggcg cgttccagat cggccggctc gaaggccgtc agcgccagcg
     8341 cctcttcgat cttgtcacgg gcggcattgg tctcgttcag aacacgttcg agcgtctccg
     8401 ccacgccggt cagcacggga ccgccggagt ccgcctggcg ctcgagccgg cgcaacgccg
     8461 aagcaaggcg ggcacccgcc gtcccctccc cttggagcgc gtccattgcc tcgttcaagt
     8521 cgccggcgat cttctcagcg ttcatcatga gctgacgccg cgaggccagc gcctcttcct
     8581 cgtccgcctc aggcgccaag gtctgcagct cctcaagcgc atgcgccacg tattcggcat
     8641 tggcacgggt ggcggcgatc tcgctttcgt ggcgggcgcg ggcggcgtcc gcctcgcccc
     8701 acgcctcgta gagttcggca agcttggcgg cgtccgcccc cagtcccccg aacgcatcga
     8761 tgagatcgcg atgcccgctc ggatcgatca gcgcccgctc gtcatgttgg ccgtggatct
     8821 cgaccagcgc gcgccccact tggcgcaaga gctgaacgct gacgcccgtg tcattgatga
     8881 aggcccggct tcggccgtca ttgccttgca cgcggcgcag gatgagcacg tcatcttcga
     8941 ttgcgatgcc gttttcggcg agcagtgcca gaactggatg gtccgccgcc agttcgaagg
     9001 tcgccgtcac ttggccctgc tccacgccac ggcggacgag cgaagcatcg ccccgcgcac
     9061 cgatggccag agagaacgca tcgagcagga tggatttgcc tgcgccggtc tcgccggtca
     9121 gcgcgctgag ccctgcgcca aactccaggt cgagcttgtc gatcagaacg atgtcgcgga
     9181 tcgacagggc ggctagcatg acacgcccgt taggcttcgc cgccgccgtt ccagactcgg
     9241 gtgatccagg agccttcgta ctgttggggt tccagtccgc gctttcctag gagacggtag
     9301 gcgtgcttgt accacttact gtccggaaag ttatggccga gcacggcggc agccgtctgg
     9361 gcctcgttga cgatacctaa ggccatataa gcctccgtca aacgcatcaa ggcctcttcg
     9421 acctgttctg tggtttgata cgacgtcacc acggtgcgga agcggttgat tgccgcgaga
     9481 aagttgttcc ggcgcaggta gtaacggccg accgtcatct cgttggcggc cagcagatcg
     9541 cgcaggattc gcgcccggtt gctcgcctca cccgaatagc gtgagttggg gtagcgctgc
     9601 agcagcttgt cgtacgccgc caaaccccgg cgcgcgaagg tctgatcccg cttcgggtcc
     9661 agcacccggt catagtagga catgccgatg atgttctgcg ccatggccga ttcttgcgtg
     9721 cccggatgga gggtcagata gcggtcggcc gccccgatgg cctcatcata tttgcccgct
     9781 ttgtaatagg cgtaggccga catgacgatg gcctgccgcg cctccgggga ataggggtga
     9841 ttgatatcga cctgctcgta tttgcgcgcg gcctcctggt agtcgccctc gttgatcagg
     9901 gtttccgcat cgccgtagat ggcgttcggc gggctggcgc tcactatttc ggcgctgtca
     9961 tctccgctgc cgaaaatcga gctaccgcag ccggccagaa gcccggcagc caaacaggtg
    10021 ccaagcccac accagcgcgc aactcgcggc gcgaacgccc gacgcggctc ttccggactg
    10081 accatgctac ccctcaaaac cgtccccctc gcgcatcggt ctttgcgcgc aaaatccgcc
    10141 cacggcaagc gctaacgcgg gcgagcccca ccaaatcccc ggattatggt gtttttttgc
    10201 tctgaccggg catcagcccg acgcacagca atagtgtcgg cggcgaaccg ccccaagacc
    10261 tgtcttcttc gaaagtggac gctccgctag ctgcggtcgg cagcgaagtt cgctaccgcc
    10321 aggcgttggc tgagctcggc atggcgcact tcgcggtagc gcggcgcctc gaccatggtc
    10381 caggcatcgg catcggcgaa cagcgccttg aggaccagcg cattcaagcg gtgtccccca
    10441 tgccgggagc gataggcgcc gagaatggga gcgccagcca aggcaagatc gccaaccgca
    10501 tcgagcgcct tgtggcggac gcattcgtcg gcgaagcgga ggccttcacg gttgataatc
    10561 cggtcatcgc cgatggccac ggtgttctca agggaggcac ccaaggccag acccgccgcc
    10621 cacagccgct cgacatcctt catgaaaccg aaggtccggg cgcgggaaat ctcgcggcgg
    10681 aaggagccgg gattgacgtc catctcaagc ttttgcttgc cgatcagctt cgtctcgtaa
    10741 tcgatctcga cgtcgagatg gaagccgtta tgcgggacca gctcgccaac ggcgccgccg
    10801 tcctccacgc agatcggctt cagaaccttc aagaagcggc gcggcgcatc gagctcggtc
    10861 agcccaacct cgtcgatcgc ctcaacgaag ggggcggcgc taccgtccat gatcggcact
    10921 tcgacgctat cgatctcaat cagggcattg tcgacgccca ggccccgcag ggcggccaga
    10981 acatgctcga cggtcgcaac ggacgcgcca ttgccatcgc cgagctcggt gcacagggtc
    11041 acgcctgtga cccattgctg gtcagccgca atctcgatcc cgtcggaatc accgttggaa
    11101 atcaggaagc gaaggccggt atcgccatcg gccgggcaaa ttgagatgga aactggcgct
    11161 ccgctgtgga cgcctgtgcc tgtcagtgaa atttcgcagg ccagcgtcgt ctggcgtgcc
    11221 ccgatgaaac ccttcatggc ccccaatggc ccccgcgccg tctatggtcc ctacttagct
    11281 cggagaaccc gagcccccat cgagcagcgc t
//
LOCUS       DBNHVU010000189        14731 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1926493, whole genome shotgun sequence.
ACCESSION   DBNHVU010000189 DBNHVU010000000
VERSION     DBNHVU010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 14731)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14731)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14731
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1926493"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /gene="pth"
                     /locus_tag="AC16Y7_07985"
     CDS             complement(<1..424)
                     /gene="pth"
                     /locus_tag="AC16Y7_07985"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR5709357.1"
                     /translation="MKLFVGLGNPGADYALNRHNVGFMAVDAIAAVHNFPAWRHRFSG
                     LLTEGRLGGEKVLLLKPQTYMNESGRSAGEAMRFYKLDVSDVVVFHDELDLAVGKVRV
                     KVGGGVAGHNGLKSLSAHLGNDYVRVRIGVGHPGHRERV"
     gene            complement(488..1162)
                     /locus_tag="AC16Y7_07990"
     CDS             complement(488..1162)
                     /locus_tag="AC16Y7_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HIR5709358.1"
                     /translation="MAEAIELKVWSRARTGKGGAREIRREGRVPGIIYGGKEEPQNVA
                     LEVKELSKQIQTGHFQSTIFMLDMDGTKIRVIPRGLQLDPIRDFPIHVDFMRLAKDAT
                     VTVDVPVHFRNEAASPGLKRGGVLNIVRHDIPVRCPADAIPDFFEIDLTGLEIGDSIH
                     ISAVALPENVIPTITDRDFTVATIVGRAAEEEAPTEADEDAEAEEGAEAEGAEGEAED
                     KDKDEK"
     gene            complement(1417..1851)
                     /locus_tag="AC16Y7_07995"
     CDS             complement(1417..1851)
                     /locus_tag="AC16Y7_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG/HbpS family heme-binding protein"
                     /protein_id="HIR5709359.1"
                     /translation="MHVNLQNAEQVIEAARKKAVELKTKMCIAVVDSGANLKAFVRMD
                     DAWVGSIDIAIKKARTACFFGMATGDLGQLSQPAKPLYGIEHSNNGLITFPGGLPIVD
                     EDGVLVGAIGVSGSSVENDHGVAAAGVDVLGVSELPEHPWRT"
     gene            2034..3215
                     /locus_tag="AC16Y7_08000"
     CDS             2034..3215
                     /locus_tag="AC16Y7_08000"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR5709360.1"
                     /translation="MTSQDQNSVRTTIAEAATLGRVRVVLAVVLLSVFVVLQLRPGTP
                     PPQHDLLAWRDDGRLHVFVHPDCPHCHEALRFLRSHPEIAADVHDVSIPANEHLFRTV
                     ARELGLPESTLGVPLFVFGDRHVLGFDRAEMSGAALLALVESGEQSAAPPRIIDLPVF
                     GEIDPTHYSLLALTVVMGVADGFNPCAMWVLIYLISLIAGLNDRAKIWWLVGTFVVTS
                     GILYFLLMAAWLNMFLVVGYVRPLTELIALAAIGFGISHLYELVWTRGAIECEVGDIG
                     ERQRTVARIRDVVAAPVGLGSLVLVTGLAFAVNAVEFVCSAALPAIYTHMLALSDVSV
                     VGYYGYIALYVVFFMLDDLIVFGLAAFAIQKIINTRYAVLSRLAGGVVLIGLGVWMLA
                     R"
     gene            complement(3222..4154)
                     /locus_tag="AC16Y7_08005"
     CDS             complement(3222..4154)
                     /locus_tag="AC16Y7_08005"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR5709361.1"
                     /translation="MKLVAGNSNRPLAEAIAAYLDTPLARCIVRRFADMEIFVEVEEN
                     VRGEDVFIVQSTSAPANDHLMELLILIDALKRASARRITAVIPYFGYARQDRKPGPRT
                     PISAKLVANMIVRAGADRVLTLDLHAGQIQGFFDIPTDNLFAAPVMVQDINEMLPKNN
                     AMVVSPDVGGVVRARGLAKRIDAPLAIVDKRRERPGDSEVMNVIGDVSGYSCILVDDI
                     VDSGGTLVNAAEALLEKGANQVVAYITHGVLSGGAVARIMASRLKELAITDSILPSDA
                     VVNAHNMRVLPIAPLLGEAIARTAEEKSVSSLFY"
     gene            complement(4274..5149)
                     /locus_tag="AC16Y7_08010"
     CDS             complement(4274..5149)
                     /locus_tag="AC16Y7_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709362.1"
                     /translation="MGGLWDGAGSLFSGTPTLALGPIVGAPDKVGKDLTQAMIAAGND
                     RNLKIVPGTTSSDGGYALRGYLLAAPASRGSKISYIWDVNDSTGARVTRVTGEQTVSR
                     RGSNPWSGVDSSVIRSIAAKSTSQLAAELPGGRSGSNTPSVASSPSPTSTGRTTAAAA
                     TSAAARPAPKAAPKPSGVLVAPVSGAPGDGRRSLTTALKKKLYGKGVKLANGAASNVY
                     TVKGVVKLSKAGAGKENIRIDWQVIDPRGKRVGTVSQKNTIPKGSLNGPWGAIANAVA
                     GAAADKLIKIIPKSS"
     gene            complement(5426..6187)
                     /gene="pgeF"
                     /locus_tag="AC16Y7_08015"
     CDS             complement(5426..6187)
                     /gene="pgeF"
                     /locus_tag="AC16Y7_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HIR5709363.1"
                     /translation="MFVQAANLAGIDGVAHGFFTRLGGVSEDIYASLNCGLGSRDIPE
                     SIVENRARAARILGTDKLIGPYQKHSNTVVVAKKAWAKDKAPEADAVVTSKPGLAVGV
                     LTADCAPVLLCEPEARVIGAAHAGWRGALSGVCEATIEAMTSLGAQPERISAAIGPAI
                     SQGAYEVGHDYKEQFLAEDPQGESFFVIDEGSGEPHFDLPGYVAERLTQSGVGEIFDL
                     GLCTYYDETRLFSYRRSQHHGEDDYGRQISAIVLA"
     gene            complement(6225..7394)
                     /locus_tag="AC16Y7_08020"
     CDS             complement(6225..7394)
                     /locus_tag="AC16Y7_08020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5709364.1"
                     /translation="MAHLARKAEASPGRVMKAERAPHSSKSTLLAARLKARIARNGPI
                     SVADYMGACLADKDAGYYSSRQPIGADGDFITAPEVSQVFGELLGLWAVAVWQSMGEP
                     KPVTVAELGPGRGSLMLDAMRAWRSVPEFLDNVTVALVETSPVLREAQRTALYDAGVP
                     VQWHDEVGGLPQHPSILIANEFVDALPVRQLVWRENQWRERCVTIASKGGFAFCEGAP
                     IADEPLQAFGQTLNEPDGTILEIRPGADALMSALGARAQATPFAGLIVDYGHEQTVCG
                     DTLQAVSRHKFADPLEAPGDVDLTAHVDFAALKDTAAAQGLIAYGPMPQGAFLLELGL
                     EARRDQLCASATAEQRQDIISATSRLVDPAAMGILFKALALTSQGLAPPPPFGDI"
     gene            complement(7349..8173)
                     /gene="lgt"
                     /locus_tag="AC16Y7_08025"
     CDS             complement(7349..8173)
                     /gene="lgt"
                     /locus_tag="AC16Y7_08025"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR5709365.1"
                     /translation="MQLFVLTYPVIDPVAIEVGPVAVRWYGLAYMSGILLGWLYARRL
                     VARKDLWGGKPPMTVSQVDDFLLWITLGIVLGGRLGFVLFYEPSFFWNNPSQIPAIWN
                     GGMAFHGGLLGVALAVYVFSRVKGINALSLGDIASAATPFGLFFGRVANFINSEVVGR
                     TTDVPWAMVFPNTDGQPRHPSQLYEAALEGVVLFIILRIATHYYKALERPGTVFGLFL
                     IFYALFRSLIEFVREPHAGHPMDVAFLTPGIVYSVPMALIGAWLIWRARRKQAPAV"
     gene            8336..8599
                     /locus_tag="AC16Y7_08030"
     CDS             8336..8599
                     /locus_tag="AC16Y7_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HIR5709366.1"
                     /translation="MTQTTGRFFDELGKLLTDAAGAADGVRKEVDSVVRAQAERVLRD
                     LDVVQREEFEAVKAMAQKAREENELLKSRIAALEAKGKGKKGK"
     gene            8794..9291
                     /locus_tag="AC16Y7_08035"
     CDS             8794..9291
                     /locus_tag="AC16Y7_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone family
                     protein"
                     /protein_id="HIR5709367.1"
                     /translation="MASIQPTYDHFTNPVDMVEQIATTHDWTFERSAPDELTLSVAGT
                     SCDYHISLTWREDLEALHLASAFDFRVTQARLPEVHRLVAQINEQLWLGHFDLWRDDG
                     MLLYRNGLLLAGAKTHIGQCEGLLKAALEACERYYQSFQFVLWAGRSAEEALAATLLE
                     TQGTA"
     gene            9288..10109
                     /gene="proC"
                     /locus_tag="AC16Y7_08040"
     CDS             9288..10109
                     /gene="proC"
                     /locus_tag="AC16Y7_08040"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR5709368.1"
                     /translation="MTLRLAGPLLLVGAGKMGGALLEGWLKQGLDPATVLIQDPALSD
                     GMRAFARQHGIVAEDAPQLPAPPAVVVIAVKPDVAAALLREMEPLLGEETVILSIAAG
                     RTLESLSRDLPDTVAVVRAMPNTPAAVGRGITVCVANANVTAAQKRECDALLASVGEV
                     VWVEDEALIDPVTAVSGSGPAYVFLFAECLEAAGIEAGLPHDLAKQLARATVAGAGEL
                     LRRSDLEPGDLRKNVTSPKGTTAAALKVLMGDEALKDLMARAVAAAAKRSGELSS"
     gene            10195..10668
                     /locus_tag="AC16Y7_08045"
     CDS             10195..10668
                     /locus_tag="AC16Y7_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709369.1"
                     /translation="MFNEFSKRNKAVRAALVLAEQRDWGDVTLPDIARHAGLDLADLR
                     REFACKSGILRAFQSEVDAEVLSKSKPGPDQSVRDRVFDMVMTRFEVMTPYKPALKRI
                     ACHLRCRPGEASALACSTLGAQYWMLAGAGAKLDGAPGAVRVAGLTAIYGRVFKV"
     gene            10702..10866
                     /locus_tag="AC16Y7_08050"
     CDS             10702..10866
                     /locus_tag="AC16Y7_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709370.1"
                     /translation="MATLDRALGNGERMLERMDKTCSMLCGLLPRGWKRSSEPKDQPP
                     ASGPSPATSV"
     gene            complement(10872..11213)
                     /locus_tag="AC16Y7_08055"
     CDS             complement(10872..11213)
                     /locus_tag="AC16Y7_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709371.1"
                     /translation="MQKTIAGLAAIAAVAFAGPALAQSETERIQETEQPGSELELNLD
                     SDGAVEFGRKGTLDAQDGVFGPPESDPLQLGGPLGERGLPLGPNEIDAELPGEGPESL
                     SGPDDDDPLPY"
     gene            complement(11235..12614)
                     /locus_tag="AC16Y7_08060"
     CDS             complement(11235..12614)
                     /locus_tag="AC16Y7_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5709372.1"
                     /translation="MTAISNTDALPRERRRPFRWLLAQFGDRMPKGLYARALIIIIAP
                     VVLLQSVVTFVFLERHWQTVTYRLSTSTVQNIALLIDLYEEFPNKQDVATLTNLAQNN
                     LGMRVRFAPGEELPQARSKPFFDLLDTTLSDGLKRRIKRPFWIDTVGRSDYVDIRVKL
                     DDAVMHVLARRSQTYASNSHIFLVWMLGTSLVLLTVAILFLRNQIKPILRLAHAAESF
                     GKGRPMPADFRPRGAREVRQAAHAFIEMRDRIERHVEQRTVMLAGVSHDLRTILTRFK
                     LQLAMFEETPELEAMHADVDEMQAMLEDYMAFAKGDSGEAIGHVDIGEVLGEVKDHAH
                     GDKTIDIAIENAPLVVPVRRHAFKRSITNLVNNAARFADHVTVSAAKHNGTLTVEVED
                     DGPGIPEDEREHVFRPFYRVDHARNQDSGSTGLGLAIARDIARIHGGDIALSQSSLGG
                     LKAVLKVPV"
     gene            complement(12614..13327)
                     /locus_tag="AC16Y7_08065"
     CDS             complement(12614..13327)
                     /locus_tag="AC16Y7_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5709373.1"
                     /translation="MPTAIKTHRGDGPDDNAPHILVVDDDSRIRDLLARYLHDHGFRV
                     TTAIDAETARASMRGLSYDLLILDVMMPKESGIDFAKALRENNQVPILMLTARAEPEQ
                     RIEGLETGVDDYLAKPFDPRELLLRVGNILRRGTSTPATGEIRMGDFVFHVSRGELQR
                     DGETVRLTERERELLRYFARHPGTPVSRHELAKGSDSGGERAVDVQINRLRRKIENNP
                     TNPVYLQTVRGKGYILYSE"
     gene            complement(13331..13918)
                     /locus_tag="AC16Y7_08070"
     CDS             complement(13331..13918)
                     /locus_tag="AC16Y7_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR5709374.1"
                     /translation="MTDLNAPMGRGRKAAGDDPGKGTPEGKGTTVPEAKLQEFAELLF
                     FAYRDFTGDPDAILKDFGFGRAHHRVLHFVNRHSGLRVADLLELLNITKQSLSRVLKQ
                     LIDQGYIAQQAGASDRRERLLFPTQRGRALAERLAAPQMVRLANALEAAGPGAEAALR
                     RFLEAMINAEDRPKVSSIMSASPADPENGDKGKAT"
     gene            14111..>14731
                     /locus_tag="AC16Y7_08075"
     CDS             14111..>14731
                     /locus_tag="AC16Y7_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR5709375.1"
                     /translation="MSGFDQREGVIWFNGELIPWTDAKVHLLTHALHYGSAVFEGMRA
                     YDGEIFKLTEHNQRLHTSAEILDFEVPYSVAELDKAARDVMRANNLSDAYVRPFAWRG
                     SEQMGVSAQKTTINVAIAAWNWGSYFDPAAREKGLKFTFAKYRRPDPATAPVHSKAAG
                     LYMICTIEKHRAEREGFADAVMLDWRGQIAECTGANIFFVKDGVIYT"
BASE COUNT         2570 a         4474 c         4884 g         2803 t
ORIGIN      
        1 caacacgctc gcgatgaccg ggatggccga cgccgatccg aacgcggaca tagtcgttgc
       61 ccaaatgcgc gctcagcgac ttcaatccgt tatgtccggc gacaccgccg ccaaccttca
      121 cgcgcacctt tccgacggcc aggtcgagct cgtcgtggaa cacgaccacg tcgctgacat
      181 cgagcttgta gaagcgcatg gcttcgcctg cggagcggcc cgactcgttc atgtaagtct
      241 ggggctttag tagcagcacc ttctcgcccc ccagccggcc ctcggtgaga agccctgaga
      301 agcggtgacg ccaggcaggg aagttgtgga cggcggcgat ggcatcgaca gccatgaaac
      361 cgacattgtg acggttgagc gcataatccg cccccggatt gccgaggccg acaaagagct
      421 tcattgcgtc atcccgatcg gtggcctgga agcatcgggg ccacaaggcc ccaaccaagc
      481 agcaggacta cttctcgtcc ttgtccttat cctcggcctc accctcagca ccttcagcct
      541 cggcgccttc ttcagcctcg gcgtcttcat cagcctcggt gggtgcttcc tcttcagccg
      601 cgcggccgac aatggtcgcc acggtgaagt cgcggtccgt aatggtgggg atcacatttt
      661 ccggcagcgc gacggccgag atatggatgg aatcgccgat ctcaagcccc gtgaggtcga
      721 tctcgaagaa gtccggaata gcgtccgccg ggcagcgcac gggaatatcg tggcgcacga
      781 tgttcaacac gccgccgcgc ttcaggcccg gagaggccgc ctcgttgcgg aagtgcaccg
      841 gcacgtccac ggtcacggtg gcatccttcg ccagacgcat gaagtcgacg tgaatgggga
      901 agtcgcggat agggtccagc tgtaggccac gcgggatcac gcggattttg gtcccgtcca
      961 tgtccagcat gaagatcgtg ctttggaagt ggccggtctg gatctgcttg gagagctctt
     1021 tgacctccaa ggcgacgttt tgcggctctt ccttgccgcc atagatgatg ccgggaacgc
     1081 gcccttcgcg gcggatctca cgcgcgccac ccttgccggt gcgcgctcgc gaccacacct
     1141 tgagttcgat cgcctcagcc atttcgtcct ccaagctcag aaataatgag ggggccggaa
     1201 accggccccg caagtcgggt gcggcgctgt gttcggcgcg ccgcagcgga atttgagagg
     1261 cttatagccc aagcgcccac gggaacacaa tcgcctatag gccctggcgg gcgccgccgt
     1321 ccccgggggc tccaccgacc ccccggggcg agcagccgac agcccttttt tggccttttc
     1381 cgcccctttg accggccgca ggaccggcgc caggggctag gtgcgccagg gatgttctgg
     1441 cagttcgctg acgccaagca cgtcaacgcc cgcggcggcg accccatgat cgttctccac
     1501 ggagctgccg ctcacgccga tggcgccgac cagcacgccg tcctcatcga cgatcggcag
     1561 gccgccggga aaggtgatca agccgttgtt ggagtgttcg atgccataga gcggcttggc
     1621 cggctgcgac agctgaccga ggtcgccggt cgccatgcca aaaaagcagg ccgtccgcgc
     1681 cttcttgatg gcaatgtcga tggagccgac ccaagcgtcg tccatgcgga cgaaggcttt
     1741 gaggttcgcg ccggaatcga ccacggcgat gcacatcttg gtcttgagtt cgaccgcctt
     1801 ctttcgggcc gcttcgatga cttgctctgc gttctgaaga ttgacgtgca tgaaatggtc
     1861 tcctcgctgg cgtgttggga ggtctgctta cagcgctttc aagacaagag gtcaacgtct
     1921 caggacttgg tttgtcggta tggagcctag gcactgaggg cctcggcccc ttgatcgagg
     1981 tcataaatgg tcgcggcttg tcctcctagg gttgtgacga ttttggagtg gccatgacga
     2041 gccaagatca gaacagcgtt cgcacaacca tcgccgaggc ggccacgctg ggccgcgtgc
     2101 gggtggtact tgcggtagtg ctcctcagcg tctttgtcgt ccttcaattg aggccaggga
     2161 cgcccccgcc gcagcacgat ctccttgcct ggcgtgacga tgggcgcctc cacgtcttcg
     2221 tccatcccga ttgtccgcat tgccatgagg cgctgaggtt cctgcgctct catccggaga
     2281 ttgctgctga tgtgcacgac gtctcgatac cggcgaacga gcatttgttc cgcacggtgg
     2341 ctcgtgagct tggtctgccc gaaagcacgc tcggcgttcc gctgttcgtg ttcggtgatc
     2401 ggcacgtttt gggcttcgac cgggcagaga tgtctggcgc ggcgcttctt gcgctggtcg
     2461 agagtgggga acaatcggcg gccccgcccc gcatcatcga tttgccggtc ttcggagaga
     2521 tcgaccccac gcactactcc ttgttggcgc tgacagtggt gatgggggtg gccgacgggt
     2581 tcaacccttg cgccatgtgg gtgctaatct acctaatctc gctgattgcc ggactgaatg
     2641 accgtgcgaa gatctggtgg ctggtgggga ccttcgtggt gacgtcgggc atcctctatt
     2701 tcctgctgat ggcggcgtgg ctcaacatgt tcctggtggt gggctatgtg cgccccctga
     2761 ctgagctgat cgcgcttgcg gccatcggct tcggcatcag tcatctttac gagctcgtct
     2821 ggacgcgcgg cgcgatcgag tgcgaggtcg gcgatattgg cgagcgccaa cgcactgtgg
     2881 cgcgcatacg cgacgtggtc gccgctccgg tcgggcttgg cagccttgtg ctggtcacgg
     2941 gactggcgtt tgcggtcaat gcggtggagt tcgtttgctc ggccgcgctc cctgcgatct
     3001 acacgcatat gcttgcgctg agcgatgtct cggtagtcgg ctactacggg tacattgcgc
     3061 tctacgtcgt gttcttcatg ctggatgatc tgattgtctt cggtctggcg gctttcgcca
     3121 tccaaaagat catcaatacg cgctatgccg tgttgagtcg gcttgcgggc ggcgtggtgc
     3181 tgattggtct cggcgtatgg atgctggcgc ggtaggggcg tctagtagaa caggctcgat
     3241 accgactttt cttccgcggt gcgggcgatg gcctcgccga gcagcggcgc gatgggcagc
     3301 acgcgcatgt tgtgcgcgtt cacgaccgca tcgctcggca ggatggagtc ggtgatggcc
     3361 aactccttca gccgtgaggc catgatacgt gccacggcgc cgccggacag tacgccgtgg
     3421 gtgatgtagg cgacgacttg gttcgcgcct ttctcaagca gggcttctgc cgcattgacc
     3481 agcgtgccgc cggaatcgac gatgtcgtcg acgaggatgc agctatagcc ggagacgtcg
     3541 ccgatgacgt tcatgacctc cgaatcgccg ggccgttccc ggcgcttgtc gacgatggcg
     3601 agcggcgcgt cgatgcgttt ggcgagaccg cgcgcgcgga ccacgccgcc gacgtccggc
     3661 gaaacgacca tggcgttgtt cttgggcaac atctcattga tgtcctgcac catgaccggc
     3721 gcggcaaaga gattgtccgt ggggatgtcg aagaagcctt ggatctgccc agcatggagg
     3781 tccagcgtca gcacgcggtc ggcgcccgcg cggacgatca tgttggccac cagcttggcg
     3841 gagatcgggg tgcggggtcc gggtttgcga tcctgccggg cgtaaccgaa atacggaatg
     3901 actgccgtga tgcgccgggc cgaggcccgc ttcagagcat cgatgaggat cagcagctcc
     3961 atcaggtggt cgttggccgg cgcggaggtg gattgaacga tgaagacgtc ctcgccgcgg
     4021 acattctcct cgacctcgac gaagatctcc atgtccgcga agcggcgcac gatgcagcgg
     4081 gcgagggggg tatccagata agcggcgatc gcctcggcga gagggcggtt gctgttgcct
     4141 gcaaccagtt tcatgcgacg gttccttgaa acccagccta ggcgcgggga gccgcgcggg
     4201 cccccgctag agcgcgcttt taacaagcgt gtggcactta tgtaaactca ccaagcgcgc
     4261 cttgtggatg aggtcagctc gatttgggga tgatcttgat gagcttgtcg gccgcggcgc
     4321 ctgcgaccgc attggcgatg gcgccccagg gcccgttcag agagcctttg gggatggtgt
     4381 tcttctgaga cacggtgccg acccgcttgc cacggggatc gatcacctgc caatcgatgc
     4441 ggatgttctc cttgcccgcg cccgccttcg acagcttcac gacgcctttg accgtgtaca
     4501 cattgcttgc ggcgccgttg gcgagcttca cgcccttgcc atagagcttc ttcttgagag
     4561 cggtggtcag ggatctgcgg ccatcaccgg gagcgccgga cacaggcgct accagaacgc
     4621 cggagggctt gggcgctgcc ttgggtgcgg gccgggcagc agcggacgtt gcggccgcgg
     4681 ccgtggtgcg tccagtggag gtcggggacg ggctcgaggc gaccgacggg gtgtttgagc
     4741 cgctccgccc gccaggcagc tcggcggcga gttgcgaggt ggacttggcg gcgatggagc
     4801 gaatcactga ggaatcgacc ccgctccagg gattgctgcc acggcgcgat accgtctgct
     4861 ccccggtgac gcgggtcacc cgggcgcccg tggagtcgtt gacgtcccaa atgtaggaaa
     4921 tcttggaccc gcggctggct ggtgcggcga gcaggtaacc gcgcaaggca tagccaccgt
     4981 cgctactggt ggtgccgggg acgattttca gattccggtc gttgccggca gcgatcatgg
     5041 cttgggtgag gtccttgccg accttgtcgg gcgcaccgac aattggcccc agggcaagcg
     5101 ttggcgtgcc gctgaagagg cttccggcgc cgtcccagag cccgcccatg ctcgcgctgc
     5161 cgcccccgca gcccgtaagg cctaaggctg cgagcgcgga gatcgcaatc cccaaaaagg
     5221 cacagaaaag gccgcgtccg tatcgtggtc ccgcgtccaa caatcgactc cctcgttggc
     5281 cgcacagcac cgctcgcata agtaaaggcg cgcgagtgga ctcccaactc tttaacaaac
     5341 caaggacctt acctgtcctg gcccgattgc gtccagatat agccgcccgg atgtcctggg
     5401 caatctgaca catcgtgaac accaatcagg caagcacgat ggcggaaatt tgacggccgt
     5461 aatcgtcctc gccgtggtgc tgggacctgc gatagctgaa cagccgggtt tcgtcataat
     5521 aagtgcagag ccctaagtcg aagatttctc cgaccccgga ctgggtcagg cgctcggcca
     5581 catagccggg caggtcgaaa tgaggctccc cggacccttc gtcgatcacg aagaaggact
     5641 cgccttgcgg atcctcggcc aggaactgct ccttgtagtc gtggccgact tcataggcgc
     5701 cttggctgat ggccgggccg atggcggcgc tgatgcgctc cggctgggcg ccgaggctgg
     5761 tcatggcctc aattgtcgcc tcgcagacgc cggataatgc gccgcgccag ccggcatggg
     5821 ccgcgccgat gaccctggcc tcaggttcgc agagaaggac gggcgcgcaa tcggccgtca
     5881 gaacgcccac ggccaagcca ggtttcgatg tgacgacagc gtccgcttcg ggcgccttgt
     5941 ccttggccca agccttcttc gccaccacca cggtgttgga gtgcttctgg taagggccga
     6001 tcagtttgtc cgttcccagg atccttgcgg cccgggcccg gttctcgacg atgctctcgg
     6061 ggatatcgcg cgaccctagg ccgcaattca atgaggcgta gatgtcttcg gagacacctc
     6121 cgagccgcgt gaagaagccg tgcgccacgc cgtcgatccc ggcgaggttc gcagcctgaa
     6181 caaacatctt gtcttcagtc accatggcct gcgctcgttt gcagttaaat gtctccgaag
     6241 ggcggcggcg gtgcaagtcc ctggcttgtc agcgccagcg ccttgaacag gatacccatc
     6301 gccgccgggt cgacgaggcg gctcgtggcc gagatgatgt cttggcgctg ttccgcggtc
     6361 gcgctcgcgc acaattggtc gcggcgcgcc tccagaccga gctcaagtaa gaacgcgcct
     6421 tgcggcatcg gcccgtaggc gatcagtcct tgcgccgcgg cggtatcctt cagcgcggcg
     6481 aagtccacat gtgcggtgag gtcgacatcc cccggcgcct cgagagggtc ggcaaatttg
     6541 tggcgggaaa cggcctgtag cgtatcgccg cacacggttt gctcgtgacc atagtcgacg
     6601 atcaggcctg cgaacggcgt ggcttgggcg cgcgcgccga gcgcggacat gagtgcgtcg
     6661 gccccgggcc tgatttcgag aatggtcccg tccggctcat tcaacgtctg gccgaatgcc
     6721 tgcaagggtt catcggcgat gggggcgcct tcgcaaaagg caaagccgcc tttcgaggca
     6781 atggtgacgc agcgctcgcg ccattggttt tcccgccaga caagctgccg gacgggcaag
     6841 gcatcgacga actcgttggc gatgaggatc gagggatgct gtggcagtcc gccgacctca
     6901 tcgtgccatt ggaccggcac gcctgcgtcg tacagcgccg tccgctgcgc ctcccgcagg
     6961 acggggcttg tctccaccag ggcgacggtc acgttgtcca agaactccgg tacgctccgc
     7021 caggcacgca ttgcgtccaa catcagcgag ccacggcccg gcccgagttc ggcgacggtg
     7081 acgggtttcg gttcgcccat ggactgccac acggcgacgg cccatagccc gagaagctcg
     7141 ccaaagacct ggctgacttc tggtgcggtg atgaagtcgc catcggcgcc aatgggctga
     7201 cgcgaggaat aatagccggc gtctttgtct gccagacagg ctcccatgta gtccgcgacg
     7261 ctgatggggc cgttccgcgc gatgcgcgct ttcagccgcg ccgcaagcag cgtgctcttg
     7321 gagctgtgcg gcgcgcgctc ggctttcatc acacggccgg ggcttgcttc cgccttgcgc
     7381 gccagatgag ccatgcgccg atcagcgcca tgggaacgga gtagacgatc cccggcgtga
     7441 ggaaggcgac atccatgggg tggcccgcat ggggttcacg cacgaactcg atcaggctgc
     7501 ggaagagagc atagaagatg aggaacaggc caaacacggt gccgggccgc tccagcgctt
     7561 tgtagtagtg agtggcgatc cgcaagatga tgaacagcac cacgccttcg agcgccgcct
     7621 cgtaaagttg gcttgggtgc cggggctgcc cgtctgtgtt tgggaacacc atggcccagg
     7681 gcacgtcggt ggtgcggccg accacttcgg aattgatgaa attggcgacg cggccgaaga
     7741 acaggccgaa cggcgtcgcg gcggaggcga tgtcgccgag cgatagcgcg ttgatgcctt
     7801 tgacgcgcga gaagacatag acggctaggg cgacgccgag cagcccgccg tggaaggcca
     7861 tgcctccatt ccaaatcgcc gggatttgcg agggattgtt ccagaagaag ctcggctcgt
     7921 agaacaggac gaacccgagg cggccgccca acacgatgcc gagcgtgatc cagagcagga
     7981 aatcgtcaac ctgactgacc gtcatgggcg gcttgccgcc ccaaaggtcc ttgcgggcca
     8041 cgaggcggcg cgcgtaaagc caccccagca gaatgcccga catataggca aggccgtacc
     8101 agcggacggc cacgggcccg acttcgattg cgacggggtc gatgacaggg taggtgagaa
     8161 cgaaaagctg catcacgact ccggttcgaa agacggggcg accctttacc gcccgaacaa
     8221 agctgtcaag gcggcaagcc tcgcccggcg cggcttgcct cgggcattgc ggcaggcaat
     8281 agtcgcaagc gatgacagtt tgttttcagc tcacgatttc aagggacttc tcgtcatgac
     8341 gcagacaacc ggacggttct tcgatgagct cggcaagctc ttgaccgacg cggcgggcgc
     8401 ggccgacggc gtgcgcaagg aagtcgacag cgtggtccgc gcgcaggccg agcgcgtgtt
     8461 gcgcgatctg gatgtcgtgc agcgtgagga gttcgaagca gtcaaggcga tggcgcaaaa
     8521 agcgcgtgaa gagaacgaac tcctgaagtc ccgcatcgcc gccttggaag ccaagggcaa
     8581 aggcaagaag ggcaagtagg ggcacggtct agagtcgagt ctgaactccg gtttatccac
     8641 agatttccca agccatccac agcaattcgc gcaatcggga ctctggcggc gttctactcg
     8701 ctgactcccc attatataac tgattcatct actgattcgc ggcagctgtt tctcgtgcgc
     8761 aggatcccct gaagaggccc tcagaggaga cgcatggctt ctatccaacc gacttacgac
     8821 cacttcacca acccggtgga catggttgaa cagattgcga cgactcacga ctggaccttc
     8881 gagcggagcg cgcccgatga gctgactctg tccgtcgcgg ggaccagttg cgactaccac
     8941 atctcgctga cgtggcggga agacctggaa gcgctgcatc tggccagcgc cttcgatttc
     9001 cgggtgaccc aggcgcggct gccggaagtc caccggctgg tggcgcagat caacgagcag
     9061 ctttggctcg gccatttcga cctgtggcgc gatgatggca tgctgcttta ccgcaacggg
     9121 cttttgcttg cgggcgcgaa gacccatatc gggcaatgcg aggggctgct aaaggcggcg
     9181 ctggaagcgt gcgagcgcta ttatcagtcc ttccagttcg tattgtgggc gggcaggtcg
     9241 gccgaagagg ccctggccgc cacgttgctt gagacgcaag gaaccgcatg acactccgcc
     9301 tcgccggtcc gctgctcctc gttggtgcgg gcaagatggg tggggcgctg ctcgagggat
     9361 ggctcaagca gggcctcgac cccgcaaccg ttttgatcca agaccccgcg ctgtcggacg
     9421 gcatgcgcgc gttcgcgcgc cagcacggca tcgtggcgga ggatgcgcct caattgccgg
     9481 cgccgcctgc cgttgtggtg atcgctgtga agccggacgt ggccgcagca cttctacggg
     9541 agatggagcc gctgctcggt gaggagacgg tcatcctatc gatcgccgcg ggacgcacgt
     9601 tggaaagctt gtcgcgcgac ctgcccgata ctgttgcggt ggtgcgggcc atgcccaaca
     9661 caccggccgc ggtggggcgc gggattaccg tgtgtgtcgc aaatgccaat gtcacggcgg
     9721 cgcagaagcg cgaatgcgat gcgctgctcg cgtccgtcgg cgaggtggtt tgggtggagg
     9781 acgaggcgct gatcgatccc gtcacggcgg tgtcgggaag cggccctgcc tatgtgttcc
     9841 tgttcgccga atgtttggag gcggccggca tcgaggccgg actgccgcac gatcttgcca
     9901 agcagctggc gcgggcgaca gtcgccggcg cgggtgagct gttgcggcgc tccgatctgg
     9961 aaccgggcga tctgcgcaaa aacgtcacct cgcccaaggg caccacggcg gcggctctga
    10021 aggtgctgat gggtgacgag gccctgaagg atttgatggc ccgcgccgtt gccgcggcgg
    10081 ccaagcgctc gggcgagctg tcctcttaag atcaatggct tttcgaccgt ctttagatgg
    10141 ccactgaaag ggcctatcct atcggccaac ggtgactgtc tgaggagatc ccccatgttc
    10201 aacgaatttt ccaaacggaa caaagccgtg cgggctgcgc tggtgctcgc tgagcagcgc
    10261 gattggggcg acgtcacgct gcccgacatc gcgcggcacg ctggcctcga tctcgccgat
    10321 ctcaggcgcg agttcgcctg caagagcggc atcttgcgag cctttcagtc cgaggtggat
    10381 gcggaagtgc tctccaagtc gaagccggga ccggaccaga gtgtgcgtga ccgcgtgttc
    10441 gacatggtga tgacgcggtt cgaggtaatg acgccctaca agcccgcgct gaaacgcatc
    10501 gcatgccatt tgcgctgccg gccgggtgag gcctcggcgc tcgcgtgctc aacgctcggc
    10561 gctcagtact ggatgctggc gggcgcgggc gcgaagctcg atggcgcacc tggggcggtg
    10621 cgggtcgctg ggctcacggc gatctatggc cgggtcttca aggtttgact ggaggacgcc
    10681 tcgccatcgc atgacaagac catggcgacg ctcgaccgcg cgctcggcaa tggcgagcgc
    10741 atgctggaga ggatggacaa gacctgcagc atgttgtgcg ggcttctgcc gcgcggctgg
    10801 aagcggagct ctgagcccaa ggatcagccg ccggcatccg ggccttcgcc ggcaacatcc
    10861 gtctgatccg cttagtaggg cagcgggtcg tcgtcgtccg ggccggagag gctctcggga
    10921 ccttcgcccg gaagttccgc gtcgatctca ttcggaccga gagggaggcc gcgctcaccc
    10981 aacggtccgc cgagttggag cgggtcgctc tccggcggtc cgaatacgcc gtcttgcgca
    11041 tcgagtgtgc ccttgcggcc gaattcgacc gcgccgtcgg agtcgagatt aagttcgagc
    11101 tccgagcccg gctgctcggt ctcctgaatt cgttccgtct ccgactgggc tagcgcgggg
    11161 cctgcgaacg ccacggcggc aatcgcagcc agtccggcaa tggtcttctg catcggcacg
    11221 tctcccaaag gactttagac aggcactttt agcacggctt tcagcccgcc gagactggac
    11281 tggctgaggg cgatgtcgcc gccatggatg cgggcgatgt cccgggcgat ggcaaggcca
    11341 agcccggtcg atccggaatc ctgattgcgc gcatgatcga cacggtagaa ggggcggaac
    11401 acgtgctccc gctcgtcttc gggaatgccc ggcccgtcgt cctcgacctc gacggtgagg
    11461 gtgccgttat gtttcgctgc gctgacagtg acatggtcgg cgaaacgcgc ggcgttgttg
    11521 accagattcg taatggaacg cttgaaggcg tggcggcgga cgggcaccac aagcggggcg
    11581 ttctcgatcg cgatgtcgat ggtcttgtcg ccatgggcat ggtccttcac ctcgcccaag
    11641 acctcgccga tatcgacatg gccgatggct tcgccggaat cgccttttgc aaaggccata
    11701 taatcctcga gcatggcttg catctcgtcg acgtcggcgt gcatggcctc gagctccggc
    11761 gtctcttcga acatcgcgag ctgtaacttg aagcgcgtga gtatggttct caggtcgtgg
    11821 ctgacgccgg caagcatgac ggtgcgctgc tcgacatgac gctcgatccg gtcgcgcatc
    11881 tcgatgaagg cgtgcgcggc ttgccggacc tcgcgcgcgc cacgcggccg gaagtcggcc
    11941 ggcatcgggc ggcccttacc aaagctctcc gctgcatgag ccaggcgcag gatcggcttg
    12001 atctgattgc gcaggaacag gatcgccacg gtcaggagca ccagcgacgt gccaagcatc
    12061 cacaccagaa agatgtgcga gttggatgca taggtctggc tgcggcgcgc cagcacgtgc
    12121 atcaccgcgt cgtccagctt gacgcgaata tcgacatagt cggatcggcc caccgtatcg
    12181 atccagaacg gacgcttgat gcggcgcttc agtccatcgg agagcgtggt gtcgagaaga
    12241 tcgaagaacg gcttggagcg cgcttgcggc agttcttcgc cgggcgcaaa gcgcacgcgc
    12301 atgccgagat tgttttgcgc gaggttcgtc aaggtcgcga cgtcttgttt gttcggaaac
    12361 tcctcgtaga ggtcgataag cagggcgatg ttctgcaccg tgctcgtgga caggcggtac
    12421 gtcaccgtct gccagtggcg ttccaggaac acgaacgtca ccaccgattg cagcagcacc
    12481 acgggcgcga tgatgatgat caaggcgcgc gcatagaggc ccttgggcat cctgtcgcca
    12541 aactgggcca gcagccagcg gaagggccgg cgtctttcgc gcgggagcgc gtcggtattg
    12601 gagatcgcgg tcatcattca gagtacagaa tatagccttt gccgcggacg gtctggagat
    12661 agacggggtt tgtcggattg ttctcgatct tgcgccgcaa gcggttgatc tgcacgtcga
    12721 cggcgcgttc gccgccggag tcggacccct tggccagttc gtggcgggag accggcgtgc
    12781 cggggtgccg cgcgaagtag cgcagcagtt cgcgctcgcg ttcggtcagg cgcacggtct
    12841 cgccatcacg ctgcagctcg ccgcgtgaga catggaacac aaagtcgccc atgcgaatct
    12901 cgcccgtggc aggtgtgctc gtgccacggc gcagaatatt gccgacacgc aataggagtt
    12961 cgcgcgggtc gaacggcttg gcgagataat cgtcgacgcc cgtttccaag ccctcgatgc
    13021 gctgctcggg ttcggcgcgc gcggtgagca tgaggatggg cacttggttg ttttcccgca
    13081 gcgccttggc aaaatcgatt ccggactcct ttggcatcat cacatcgaga ataagcaggt
    13141 cgtaagacag accgcgcatg gacgcgcgtg cggtctccgc atcgatggcc gtcgttacgc
    13201 ggaagccgtg atcgtgaaga tagcgcgcca gcaaatcgcg aatgcgactg tcgtcgtcca
    13261 ccacaagaat gtggggggcg ttgtcgtcgg ggccgtcgcc gcgatgggtc ttgatggctg
    13321 tcggcataat ctaagtcgct tttcccttgt cgccgttttc gggatcggct ggagatgcgg
    13381 acattatgga ggagactttg ggccgatctt cggcattgat catcgcctcg agaaaacggc
    13441 ggagcgccgc ctcggcgccg gggccggccg cctcaagcgc attggccaag cgtaccattt
    13501 ggggcgcggc gaggcgttcg gccagggcgc ggccgcgctg ggtggggaac aggaggcgtt
    13561 cccgacggtc ggaggcaccc gcttgctgcg cgatgtagcc ctgatcgatc aactgcttga
    13621 gcacgcgcga gaggctctgc ttggtgatgt tgagcagttc cagcaggtcc gcgacgcgca
    13681 agcccgaatg gcggttgacg aaatggagca cccggtgatg ggcgcgcccg aagccgaaat
    13741 ccttgaggat ggcgtccgga tcgcccgtga agtcgcgata ggcaaagaac aggagttcgg
    13801 caaattcctg cagcttggcc tcgggcacag ttgtgccctt gccctccggg gtgcccttgc
    13861 caggatcgtc gccggcggct ttgcggcccc ggcccatcgg tgcatttagg tcagtcatgt
    13921 tgacatattt ttccgcttgg tttatgtcag ccatgttgac ttaattcagt ccttgatacg
    13981 cctcgccgcc tgctcgcgca attgcccctt gctcgcaatt gtttaggcag atggtatgag
    14041 gggcacctga gtgattttgg gatcgaggcc gcaactcaga acggcctgtc cttggcgtgg
    14101 aggagacgtg atgtcgggct ttgatcagcg cgaaggcgtt atctggttca atggcgagct
    14161 gatcccgtgg accgacgcaa aggtgcatct tctcacccat gcgctgcatt atggcagcgc
    14221 cgtgttcgag ggcatgcgcg cctatgacgg cgagatcttc aagctcaccg agcacaatca
    14281 gcgcctgcac acctccgccg agatcctaga cttcgaggtt ccgtattccg tcgccgagct
    14341 cgacaaggcg gcgcgggacg tcatgcgcgc caataatctg tccgacgcct atgtccggcc
    14401 gttcgcctgg cggggcagcg agcagatggg cgtctcggcg cagaagacga cgatcaatgt
    14461 tgccatcgcc gcgtggaact ggggctcgta tttcgatccg gccgcgcgcg agaaggggct
    14521 gaagttcacc ttcgccaaat accgccggcc cgacccggcg accgcgccgg tccacagcaa
    14581 ggcggcgggt ctttatatga tctgcaccat cgagaagcac cgcgccgaac gcgaaggctt
    14641 tgccgatgcc gtgatgctcg attggcgcgg acagatcgcc gagtgcacag gcgcgaacat
    14701 cttcttcgtg aaggacggcg tgatctatac g
//
LOCUS       DBNHVU010000190        12138 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1258311, whole genome shotgun sequence.
ACCESSION   DBNHVU010000190 DBNHVU010000000
VERSION     DBNHVU010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 12138)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12138)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12138
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1258311"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="AC16Y7_08080"
     CDS             <1..913
                     /locus_tag="AC16Y7_08080"
                     /inference="COORDINATES: protein motif:HMM:NF019844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR5709376.1"
                     /translation="TQPREEPKKDAETPDIPAFETVTTEDGTTPAGDPSAIQDIPAQD
                     IPAASDPTTSQDMAALPGEGAPAQTPNAAAPGQPLPWATDQAEQWGDERRQQMPDQAY
                     GRPVPPANPYGQQGGPPPGQRPYGPPPGQPYGQQGGPPPGGAYGPDGSYDAYAPPRDP
                     YAAQRDPYTQGDPYSGQADPYGAPPPQQWGGQPGAPPGEQWVQVVGSGTGMRATASID
                     APILFAFPYGRQLKVVTRNDEWVEVTDPNSSAKGWMPAHALAPSAGPNAGYDYSQQQA
                     YEEQQPRRRGLFRRGGFEDMINRAFGN"
     gene            complement(932..1303)
                     /locus_tag="AC16Y7_08085"
     CDS             complement(932..1303)
                     /locus_tag="AC16Y7_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HIR5709377.1"
                     /translation="MPPSEGFKAFIVDQLAGFGPVNIRNMFGGAGIYADGVMFAILAD
                     DTLYLKADETSSHAFADEGMEPFTYTAKGGKPIAMSYWEVPPRLLEEPDELAEWAREA
                     HRIACAQKKPSRKSSKKRKSA"
     gene            complement(1316..3229)
                     /locus_tag="AC16Y7_08090"
     CDS             complement(1316..3229)
                     /locus_tag="AC16Y7_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006936571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR5709378.1"
                     /translation="MLSTAHATKRRFQLILIKPSHYDGDGYVVQWMRSTMPSNSLAAV
                     YSLAQGAAERQLLGPDLPIDVHAMDETNTRVKTKDIVRRIAENGGLGLVGIVGAQSNE
                     FPRAVDIARPLRAAGVQVAIGGFHVSGCLSMLPELPDDIKEAQALGISIYAGEAEEGL
                     DELILDTARGEMKPLYNHMPNLPDIGEIPSPPFLPVEFVRRTIGNTTSFDAGRGCPFQ
                     CSFCTIINVQGRKSRYRTPDSVEQILRMNYEQGVNRFFITDDNFARNKDWEAIYDRII
                     KLREEDGMDIRFCIQVDTLCHKIPNFMDKSKRAGVTRVFIGLENINPANLIAAKKRQN
                     KITEYRKMLLEWKRVGIMTYAGYILGFPNDTPESIRQDLEIIKHELPLDVLEFFILTP
                     LPGSEDHKILYEKGVPMDPDMNKYDVEHVCTAHAKMSKEEWQEAYRTAWDFYYTDEHL
                     ETIIRRAYATGINLKSLRVVLFWFSGAVPTEGVHPLQWGIFRMKYRRDRRSGLPLENP
                     IVFYSKYAADVARKLYMAGRRWRHLTRLMNKVESDPTAKFHMDNALTPVADEDAEHME
                     LYTQNEAARVAVERELRVAGGKPKGNGAGQPALQVKMNGNGKAAHAGNGQDSTAETVE
                     AEKRHQESAAPPA"
     gene            complement(3404..3730)
                     /locus_tag="AC16Y7_08095"
     CDS             complement(3404..3730)
                     /locus_tag="AC16Y7_08095"
                     /inference="COORDINATES: protein motif:HMM:NF019401.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HIR5709379.1"
                     /translation="MGAVIVVALGAFQAIIAVIGAIFSGNADELRGREIWLKFATWIL
                     LALEFALAADIVHTAVAPTWEDIWKLAVIAVIRTMLNYFLAKDIADFTRLQDSGQIPA
                     SKPVQS"
     gene            complement(3856..4245)
                     /locus_tag="AC16Y7_08100"
     CDS             complement(3856..4245)
                     /locus_tag="AC16Y7_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003605197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HIR5709380.1"
                     /translation="MPLLRFDLIQGRTDEELKALLDAAHRAMLEAFQVPVRDRYQIVH
                     EHKASRMIVEDTGLDIPRSDKVVFLQVTSRPRKRAQKEAFYRLLCEELERSCGIASSD
                     VVVSIVENSDEDWSFGLGRGQFLTGEL"
     gene            complement(4248..5873)
                     /locus_tag="AC16Y7_08105"
     CDS             complement(4248..5873)
                     /locus_tag="AC16Y7_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HIR5709381.1"
                     /translation="MVDPYNTGLKKTPANYQPLTPLVFLERAAAVYPDHTAIVHGGAR
                     TDYRTFYARTRRLASALQARGIGEGDTVSVMLCNSPAMLEAHYGVPMVGAVLHSINTR
                     LDAEAVAFMLAHAETKVLIGDTEFAPVLKEALARAKAKPLLIDYRDLELGVEGMRLGD
                     LDYEVLLDGGDPDFAWAMPADEWNAISLNYTSGTTGNPKGVVYHHRGAALMCYANTLA
                     TGMGTHPVYLWTLPMFHCNGWCFPWSLSLVAGTHVCLRAVRARPIYDAIAEHKVTHLS
                     GAPFVMAALLGAEPEEKRDFGHRVAFNHAAAPPPAAVLAKMDEAGFELTHLYGLTETY
                     GPATLNEWKGAWDELPPEERLKKRIRQGVRYPALEDLTVMNPETMEKTPADGKTIGEV
                     MFRGNIVMKGYLKNKTATDAAFAGGWFHSGDLGVMHPDGYIQLKDRSKDIIISGGENI
                     SSIEVEDVIYKHPDVTAAAVVAKPDDKWGETPCAFVELKDGAQTSEADIIAWCREHLA
                     HFKCPRHVVVIELPKTSTGKIQKFKLREIAKDV"
     gene            complement(6158..7009)
                     /locus_tag="AC16Y7_08110"
     CDS             complement(6158..7009)
                     /locus_tag="AC16Y7_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007008293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1013 domain-containing protein"
                     /protein_id="HIR5709382.1"
                     /translation="MTRTPLMPKATAVWLVDNTSLTFDQIAEFCGLHVLEVKGIADGD
                     VAHGIKGMDPISSGQLSRDEIAVAEEDPKHRLVLLEPKVEVPEVKTKRGPRYTPVSRR
                     QDRPNAILWLLRNHPELKDSQVMRLVGTTKPTILSIRERTHWNSPNLVAQDPVTLGLC
                     SQIDLDAAVKRAAARLERERKKAEKAAAKAGTLLPTEETTAAAVPMTPAEAHAGAASP
                     PEAPLVPPAPPTPEDAMAASLAKEARDAMAAPVEEEAAPDADSVFAKLKELKPEGGGS
                     EEDTPSE"
     gene            complement(7157..9832)
                     /locus_tag="AC16Y7_08115"
     CDS             complement(7157..9832)
                     /locus_tag="AC16Y7_08115"
                     /inference="COORDINATES: protein motif:HMM:NF017021.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR5709383.1"
                     /translation="MPVFAFLVLYAEPFEPLRRNMTEKLLSEAMDATVEVRGPIHIDA
                     GWAPTVRIEDIAAVESSLPPDLKRLSIKSIKVDVPLMPLFIADVQLRALVIEGLDVAI
                     EIPEGGADEASINVAETVGDFVRSPFASDFVLRDAKVDYVNEENGFDLHYVFDEIASH
                     PSDDGGVDVEGTGRFNDEPWRLEVDVDPPGEDEDRRKFVFAVNHAGLKVAFAGNYAFD
                     TSFFGTESDAVDMTVTASAPQLNRSLAVYDIKGELDGSGALSGRLAGSLDELALSELA
                     LKLAFESGDAYEVTGDIADLMQGTGLALKLKGAFAKKQLPEGEARPIYDIGVTGFRGR
                     IEGSRDSVLIRDFRVTTSAIRTKFRDIGPITAERLYKTDDGRLGLYDVVVLAGDPKRP
                     SVRVAGTVKDIIGFKGVSLNGQIDFLTADLFDLAAEESAEALGHLFGDVTISDADGSL
                     GIESLSATVTDSSLLELSVDLAIDDLPEANELTFATHLDIEKFKPLAAALGSEVEDVG
                     RVKFDGTVARSDENIVMAGTMLVGQTTLEGSLTGALSEKRAPVLNGNIATELLHLSDL
                     MKLASINAVFQENADDADADVIDYSEVWETLLVDLQVKVAAIAGGGGNASNIEGRMTY
                     RAGVINLDPMNMTYLGGTASTNGNIDTKDNQNSFALKGSINNLRIGEVLQAMEVNYPV
                     SGALNVTYDLSGAGNTKAQIPRTLSGSLTTSLRNGSIGTRLIDLTGMSLPGWLLSGAA
                     GGSQSPLVCVVAPFSFKNGRGTTRGLVLETNNVQVVGVGTVDFRANTIDLHFKPQALR
                     KQFIKTAQPFSIQGKLASAKVTLGGAPVAGAVVGTLASPLNLLSAITQPRAGTPGRVP
                     CRVVHAKPQSGQATTTKPRRGGPLGLGILGGQRRR"
     gene            complement(9925..10908)
                     /locus_tag="AC16Y7_08120"
     CDS             complement(9925..10908)
                     /locus_tag="AC16Y7_08120"
                     /EC_number="1.6.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HIR5709384.1"
                     /translation="MTVIEAREAGGPEVLVPAMRPAPKPAPDEVLIAVEAAGINRPDV
                     LQRQGLYPPPKGASDLLGLEVAGPVIMVGEAVTRFKPGDFVCALVNGGGYAEFAVAPE
                     TTTLKAPDGLTAVQAAALPETIFTVWHNVFERGGLEEGDWLLVHGGASGIGTTAIQLA
                     TALGAKVIVTVGDDTKAKACEALGAVRAINYREEDFVDVVRKVTEKHGADVILDMVGG
                     DYIERNLRAAALEGHIVQIAFLKGSKVEIDLMRLMMRRLTLTGSTLRAQGAKAKARMA
                     EAIEKTVWPLIAAGDFKPVIDSTFPLSDAADAHKRIDDPGHVGKIVLEVTG"
     gene            11026..11214
                     /locus_tag="AC16Y7_08125"
     CDS             11026..11214
                     /locus_tag="AC16Y7_08125"
                     /inference="COORDINATES: protein motif:HMM:NF018413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HIR5709385.1"
                     /translation="MDLDDLTPKAPKGDTLGQDLSKHSVAELEALIDALREEIARVEE
                     ALGAKQSSKNAAEAAFKR"
     gene            11509..12054
                     /gene="rcdA"
                     /locus_tag="AC16Y7_08130"
     CDS             11509..12054
                     /gene="rcdA"
                     /locus_tag="AC16Y7_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein RcdA"
                     /protein_id="HIR5709386.1"
                     /translation="MAGKSKNDSTDAYEGVTVSFGAKFARSDQFKTVFREGMALVEAA
                     AAYLDGDGRKEARKLKPPHSLAYATESMRLTTRLMQLASWLLIRRAVSEGELTPEQAV
                     EEQRKIKLPVAGAEQPTRDFEALPERLRDLIEQSIKLQERVVRLDQMLDFNEQTAEGE
                     THHPLSSQLSALHAAFGSEAE"
BASE COUNT         2124 a         3788 c         3889 g         2337 t
ORIGIN      
        1 cacccagccg cgcgaagagc cgaagaagga cgccgagacg ccggacatcc cggccttcga
       61 gaccgtcacc accgaggacg gcaccacgcc agccggcgac ccgtcggcca ttcaggatat
      121 ccccgcccag gatatccccg ccgcatccga ccccacgacg tcacaagata tggcggcgct
      181 gccgggtgag ggcgcccccg ctcaaacgcc gaacgccgca gcccccgggc agcctttgcc
      241 ttgggccacg gaccaggccg agcaatgggg cgatgagcgg cgtcagcaga tgccagatca
      301 ggcttatggc cgcccggtgc cgcctgcaaa cccttacggt cagcagggcg gtccgccgcc
      361 aggtcaacgg ccttacggac cgccgccggg tcagccttac ggtcagcagg gtgggccacc
      421 gcccggtggt gcttatggcc cggacgggtc ctacgatgct tacgcgccgc cgcgcgatcc
      481 ttacgccgcc cagcgcgatc cctacacgca aggcgatcct tattcggggc aagccgatcc
      541 ttacggcgcg ccgccgccgc agcaatgggg cggccaaccc ggcgctccac ccggcgagca
      601 atgggtgcaa gtcgtcggct ccggcaccgg catgcgggcc acggcatcga tcgacgcgcc
      661 gatcctgttc gcctttcctt acggccgcca attgaaggtc gtgacgcgca atgacgaatg
      721 ggtcgaggtg accgatccga acagctcggc caagggctgg atgccggcgc atgctttggc
      781 gccgtcggcc ggccccaacg cgggctacga ctacagccaa cagcaggctt acgaggaaca
      841 gcagcccagg cgccgcggct tgttccgccg tggcggtttc gaggacatga tcaaccgcgc
      901 cttcgggaac tgatcgctcc ggcgtttcac gtcacgctga ctttcgcttc ttggacgatt
      961 ttcgtgacgg tttcttttgg gcgcaggcaa tgcggtgggc ctcgcgtgcc cactcggcaa
     1021 gttcgtccgg ctcctccagc agacgtggcg ggacttccca ataggacatg gcgatgggct
     1081 tgcccccttt ggccgtgtag gtgaaaggct ccatgccttc gtcggcgaag gcgtgcgacg
     1141 acgtctcgtc ggccttcaag tagagcgtgt catcggcaag gatcgcgaac atcacaccgt
     1201 ccgcatagat gccggcgccg ccgaacatgt tgcggatgtt caccggtccg aagccggcca
     1261 attgatcgac gatgaatgcc ttgaagccct ctgacggcgg cattacgtcc gaggcttacg
     1321 caggcggcgc ggcgctctct tggtggcgct tctcggcctc gacggtctct gccgtgctgt
     1381 cttgaccatt gccggcgtgg gccgccttgc cgttgccgtt catctttacc tggagcgcgg
     1441 gctggcccgc gccattgcct ttgggtttgc cgccggcgac gcgcagctca cgttccacgg
     1501 cgacacgcgc cgcctcgttc tgggtgtaga gttccatatg ctcggcgtct tcgtcggcga
     1561 cgggggtcag cgcgttgtcc atatggaact tggctgtcgg atcggactcc accttgttca
     1621 tgaggcgggt caaatgccgc caccgcctgc cggccatata cagcttgcgg gcaacgtccg
     1681 cagcgtattt tgaatagaaa acgatggggt tttcgagcgg cagacccgag cgccggtcac
     1741 gccggtattt catccggaag attccccatt gcaggggatg cacgccctcg gtcggcacag
     1801 cgcccgagaa ccagaacagc acgacccgaa gagacttgag gttgatgccc gtcgcatagg
     1861 cgcgccggat gatcgtctcc aagtgctcgt cggtatagta gaagtcccaa gcggtgcggt
     1921 aggcctcttg ccactcttcc ttgctcattt tcgcgtgagc cgtacagacg tgctccacgt
     1981 cgtacttgtt catgtcgggg tccatcggaa cccctttctc atagaggatc ttgtggtcct
     2041 cggatcccgg tagcggcgtg agaatgaaaa actccagaac atcgagcggc agctcgtgct
     2101 tgatgatctc aaggtcctgg cgaatggact cgggcgtgtc gttggggaag ccgagaatgt
     2161 agcccgcata ggtcatgatg ccgacccgct tccattcgag aagcatcttg cggtactcgg
     2221 tgatcttatt ctgacgcttc ttggccgcga tcaggttggc cgggttgatg ttctcaagcc
     2281 cgatgaacac gcgcgtgacg ccggcccgct tcgacttgtc catgaagttc gggattttgt
     2341 ggcaaagcgt gtcaacctgg atgcagaagc ggatgtccat gccatcctct tcgcgcagct
     2401 tgatgatgcg gtcgtagatc gcttcccaat ctttgttgcg ggcgaagttg tcgtcggtga
     2461 tgaagaaccg gttcacgccc tgctcgtagt tcatgcgcag gatttgctcg acgctgtcgg
     2521 gcgtgcggta ccgcgacttc cggccttgca cgttgatgat ggtgcagaac gagcactgga
     2581 agggacagcc gcggcccgcg tcgaagctcg tcgtgttgcc gatggtgcgc ctgacgaatt
     2641 cgaccggcag gaacggcggc gatgggatct cgccgatatc cggcagattc ggcatgtgat
     2701 tgtatagcgg cttcatctca ccgcgcgcgg tatcgaggat cagctcatcg aggccctctt
     2761 cggcctcgcc cgcataaatg ctgatgccga gcgcctgcgc ttccttgatg tcgtcgggaa
     2821 gctccggaag catcgacaag cagcccgaaa catggaaccc gccgattgcg acttgcacgc
     2881 cggccgcgcg cagaggtcgg gcgatgtcca cggcacgggg aaattcgttc gactgcgcgc
     2941 cgacaatccc aaccagcccc aggccgccgt tttcggcgat gcggcggacg atgtctttcg
     3001 tcttcacgcg ggtgttggtc tcgtccatgg cgtggacgtc gatgggaagg tcggggccca
     3061 gcagctgccg ttcggcggcg ccttgcgcga ggctgtatac cgcggcaagc gaatttgacg
     3121 gcatggtcga gcgcatccat tgcacgacat agccgtcgcc atcgtagtgc gacggcttga
     3181 tcagaatgag ctgaaagcgc cgtttcgtcg cgtgtgctgt actgagcatc cctcaatccc
     3241 gtgaagaggc gtctcccgcg cgggaagcgg gactttagtg ccgctctgaa aatggacgcg
     3301 ccgcgcccct accccctgcc ggcgggcgct tacggaacga tttttccgca cgccataaga
     3361 ctttggccca ctgaaccata gaatgggtca acggtccaac ggattaggat tgaacgggct
     3421 ttgaggcggg aatttggcca gaatcctgga gtctggtgaa atccgcgatg tccttggcca
     3481 ggaagtagtt cagcatggtc cggatcacgg cgatcacggc caatttccag atgtcctccc
     3541 aggtcggggc gacggccgta tggacgatgt cggcggcgag cgcgaattca agcgccagca
     3601 ggatccaggt ggcaaatttc agccaaatct cgcggccccg gagctcgtcc gcgttgcctg
     3661 agaagatcgc gccgatgacg gcgatgatcg cctggaaggc gccgagcgcg acgacgatga
     3721 cggcgcccac ctcgatggaa agcgcgatgt agttcgcgat tgtgctgaag ctctcctcga
     3781 tcggaatggc gccgtcctcc cctttaaggc gtggggaagt taagccgatt cgcccctttg
     3841 cctcaccctc tcgggctaga gctcgccggt caggaactgg ccgcggccca agccaaatga
     3901 ccagtcctcg tccgaattct cgacgatgga gaccacgacg tccgacgagg cgatgccgca
     3961 ggaccgctcg agctcctcac acaggagccg gtagaaggcc tccttttgcg ctcgcttgcg
     4021 cggccgggag gtaacttgca gaaacacaac cttgtcgcta cgcggaatgt cgaggccggt
     4081 gtcctcgacg atcatccgtg aggccttgtg ctcgtgcacg atctgatagc gatcgcgcac
     4141 cggcacttga aaggcttcga gcatggcccg atgggcggca tcgagcagag ccttgagctc
     4201 ttcgtcggtg cggccttgga tgagatcaaa gcggagcagg ggcattgtta aacgtccttg
     4261 gcgatttcgc gcagcttgaa tttctggatt ttgccggtcg aggtcttggg tagctcgata
     4321 acgaccacat ggcgcgggca tttgaagtgg gcgagatgct cgcggcacca ggcaatgatg
     4381 tcggcttcgc tggtttgcgc gccgtccttc agctcgacga aggcgcacgg tgtctcgccc
     4441 catttgtcgt cgggcttggc caccacggcc gcggcggtga cgtcggggtg tttgtagatg
     4501 acgtcttcga cctcgatgga ggagatgttc tcgccgcccg agatgatgat gtccttggag
     4561 cggtctttga gctggatgta gccgtccggg tgcatgacgc cgagatcgcc ggaatggaac
     4621 cagccgccgg cgaacgccgc gtcggtcgcc gtcttgttct tgaggtagcc cttcatgacg
     4681 atattgccgc ggaacatgac ctcgccgatg gtcttcccat cggccggtgt cttctccatg
     4741 gtctccgggt tcatgacggt caggtcttca agcgcgggat agcgcacgcc ttggcggata
     4801 cgtttcttaa gccgctcttc ggggggcagc tcgtcccaag cgcccttcca ctcgttgagg
     4861 gtcgcaggcc cgtaagtctc ggtcagcccg taaagatggg tcagctcgaa gccggcttcg
     4921 tccatcttcg cgagcaccgc ggcgggcggg ggcgcggcag catggttgaa ggcgacgcga
     4981 tggccgaagt cgcgcttctc ttccggctct gcgcccagga gcgcggccat gacgaacggc
     5041 gcaccggata gatgcgtcac cttgtgctcg gcgatggcgt cgtatatggg cctagcgcgc
     5101 accgcccgta agcagacatg cgtaccggcg acgaggctga gcgaccaggg gaagcaccag
     5161 ccgttgcagt ggaacatggg cagcgtccaa agatagacgg gatgcgtgcc catgccggtg
     5221 gcgagggtgt tggcgtagca catcagcgcc gcgccgcgat ggtgatagac cacgcctttg
     5281 gggttgccgg tggtgccgct cgtatagttg agcgaaatgg cattccattc gtcggctggc
     5341 atcgcccagg cgaagtcggg atcgccgccg tcgagaagga cctcgtagtc gagatcgccg
     5401 aggcgcatcc cttcgacgcc tagttcgaga tcgcgatagt cgatgagaag cggctttgcc
     5461 ttggcgcggg caagcgcctc cttcagcacg ggcgcgaact ccgtgtcccc gatcagcacc
     5521 ttcgtctccg catgggcgag catgaaggcg acggcctcag cgtcgaggcg cgtgttgatc
     5581 gagtgaagca ccgcccccac catgggcaca ccgtaatggg cttccagcat ggcgggcgag
     5641 ttgcacagca tcaccgacac cgtgtcgcct tcgccgatgc ctcgggcttg aagcgcggag
     5701 gcaagccggc gcgtgcgcgc ataaaatgtg cggtagtcgg tccgcgcgcc cccatgcacg
     5761 atcgcggtgt gatccggata cacggcggcg gcgcgctcca ggaacacgag cggcgtcaac
     5821 ggctggtagt tcgccggggt cttcttcaga ccagtgttgt agggatcgac catgggcttg
     5881 gcgggcgcgt ttcctcgatg cacactagcc aagcaggtgg tcttgaatca attgctgttg
     5941 ctcgcccttt cggggcggat gaccggctag cggtgtgtgt aataggccaa tgtcacggca
     6001 aacgcgccga acgcaccgac cacagccacc aaaaagagaa tttcgaactc gctcataatg
     6061 tctccccctc catgcgcatt ttttggaagg gggaggctcg cctctggccg ggcgtccagc
     6121 cttgatcgag gtcaaatcgg ggtgtgtccc cgcattctta ctcggaaggg gtgtcctctt
     6181 cggatccgcc gccctcgggc ttcagctctt tcagcttggc gaacacggag tcggcgtccg
     6241 gcgcggcctc ttcctcgacc ggagcggcca tggcgtcgcg ggcttccttt gccagcgagg
     6301 cggccatggc gtcttcaggc gtgggtggcg ctggcggcac tagcggtgcc tcgggcgggc
     6361 ttgccgcccc agcatgggct tccgcgggcg tcatgggaac ggcggcggcg gtcgtctcct
     6421 cggtaggcag gagcgtgccg gccttggcag cggccttctc ggccttcttg cgctcgcgct
     6481 ccagccgcgc cgcggcccgt ttcacggccg catcgagatc gatctgtgag cacagcccaa
     6541 gcgtcaccgg atcctgcgcc accagattgg gcgaattcca gtgggtgcgc tcacgaatgg
     6601 acaggatggt cggcttggtg gtgcccacca ggcgcatgac ctggctgtcc ttcagctcgg
     6661 ggtggttcct caagagccac aggatggcgt tcggacggtc ctggcggcgc gacacgggtg
     6721 tgtagcgcgg gccccgcttg gtcttgacct cgggcacctc gaccttgggc tccagaagca
     6781 ctagccggtg cttcgggtct tcctcggcca ccgcgatctc atcgcgggag agctggccgg
     6841 aggagatggg gtccatgccc ttgatgccgt gggccacgtc gccgtcggcg atgcccttca
     6901 cctcaagcac gtgcaggccg cagaattcgg cgatctgatc gaaggtgagg gaagtgttgt
     6961 cgaccagcca aacggcggtc gccttgggca tcaaaggggt gcgggtcatg atgggttttc
     7021 agccttgctt aaaggggtag cgccgtctct ctcgccccct cggcgaaaga gccaaatcaa
     7081 ccaattgagg ggaatgagcc ctatataggg acactcgccc ccacaagcaa ttggttctgg
     7141 gagccgtcct ggccgatcaa cggcgcctct ggccgccgag aatgccaagg ccgagcggac
     7201 caccgcgccg cggcttggtg gtcgtggcct ggccgctctg cggcttggca tgcaccacgc
     7261 ggcagggcac gcgcccgggc gtgccggccc ggggctgtgt gatcgcactc aggaggttca
     7321 ggggcgaggc aagtgtgccg acgaccgcac ccgccacggg cgccccgccg agcgtcactt
     7381 tcgcgctggc gagtttgccc tggatggaga agggttgcgc ggtcttgatg aattgcttcc
     7441 gtagcgcttg cggcttgaag tgaagatcga tggtgttcgc gcggaaatcc accgtgccca
     7501 cgccgaccac ttgcacattg tttgtctcga gcaccaagcc tcttgtcgta ccgcgcccgt
     7561 tcttgaagga gaagggcgcg acaacgcaca cgagcggcga ctgactgccg ccagccgcgc
     7621 ccgacaggag ccagcccggc aggctcatgc ccgtcaggtc gatcaggcgt gtgccgatcg
     7681 agccattgcg cagcgatgtc gtgagcgagc cgctcagcgt ccggggaatc tgcgccttgg
     7741 tgttgcctgc gccagacaga tcgtaggtca cgttgagcgc cccggagacc ggatagttga
     7801 cctccatcgc ctggagcact tcgccgatgc gcaaattgtt gatgctgccc ttgagcgcga
     7861 agctgttctg attgtctttg gtgtcgatat tgccgttggt ggaggctgtg ccgccgagat
     7921 aggtcatgtt catggggtcg aggttgatga ctccagcgcg gtaggtcatg cggccttcaa
     7981 tgttgctcgc attgccacca ccgccggcga tcgcggccac ctttacctga aggtcgacga
     8041 gcaaggtctc ccagacctcc gaatagtcaa tcacgtcagc atccgcgtcg tcggcattct
     8101 cctgaaacac ggcgttgatg gacgcgagct tcatgagatc cgacaaatgc agcagctcgg
     8161 tcgcgatgtt gccattgagg acgggcgcac gcttttccga gagcgccccg gtcagcgaac
     8221 cttcgagcgt ggtttgcccg acaagcatgg tcccggccat gacgatgttc tcatcgcttc
     8281 gggctacggt gccgtcgaac ttgacccgcc ccacgtcctc aacttcgctt ccaagcgccg
     8341 cagccaacgg cttgaacttc tcaatgtcga ggtgggtcgc gaatgtgagc tcattggcct
     8401 ccggcaggtc gtcgatcgca aggtccacgc tgagctcgag caggctgctg tcggtgaccg
     8461 tcgccgacag gctctcgatg ccgagcgatc cgtcggcatc ggagatggtc acgtcgccaa
     8521 aaaggtgacc aagcgcttcg gcgctctcct cggccgcgag gtcgaaaagg tcggccgtga
     8581 ggaaatcgat ctgcccgttg aggctgacgc ctttgaaccc gatgatgtct ttcaccgtgc
     8641 cggcgacgcg cacgctcggg cgcttgggat cgccggccag gaccacgacg tcgtagaggc
     8701 cgagcctgcc gtcgtcggtc ttatagagac gttcggcggt aatcggcccg atatccctga
     8761 acttggtccg gatggcgctc gtggtcacgc ggaaatctct tatgagaacg ctgtcccgcg
     8821 acccctcgat gcgccctcga aagccggtga cgccgatatc gtagatcggc cgtgcctcgc
     8881 cttccggcag ctgcttcttc gcgaacgcgc ccttgagctt cagcgcgagg cccgtgcctt
     8941 gcatgaggtc tgcgatgtcg ccggtcactt cgtaagcgtc cccggactcg aatgcgagct
     9001 tcagcgcgag ctcgctgagc gcaagctcat ccagcgatcc tgcaagccgc cccgaaagcg
     9061 caccggatcc atcaagctcg cccttgatat cgtagactgc gagagaccgg ttcaactgcg
     9121 gcgccgatgc ggtcaccgtc atgtcgacgg catcgctttc cgttccgaag aagctcgtgt
     9181 cgaaggcata gttgcccgcg aaggcaacct tcaggcctgc atggttcact gcaaacacga
     9241 acttccgccg gtcttcgtcc tcgcccggcg gatcgacatc gacttcgagc ctccatggct
     9301 catcgttgaa ccggcccgtt ccctcgacat cgaccccgcc gtcgtccgat gggtggctcg
     9361 caatctcatc gaagacatag tggagatcga atccgttctc ctcgttgaca tagtcgactt
     9421 tcgcatcgcg caagacgaag tcgctcgcga atggcgagcg gacgaagtcg cccaccgtct
     9481 cggccacgtt gatgctggct tcgtctgcgc cgccttccgg aatctcgatg gccacgtcga
     9541 gcccctcgat cacgagggcg cggagttgca catcggcgat aaacaacggc atgagcggca
     9601 cgtcgacctt gatcgacttg atcgataacc gcttgaggtc cggcgggaga ctgctctcca
     9661 ccgccgcgat gtcttcgatc ctcacggtcg gcgcccagcc ggcgtcaatg tggatgggcc
     9721 cgcgcacctc aacggtggcg tccatcgctt cagagagaag cttctcggtc atattccggc
     9781 gtagcggctc gaagggctcc gcgtagagca ccaaaaacgc gaacactggc acacaaagga
     9841 tcaacccgcc gatgaagagg actcgaagga agaaccgaac tgaggaattg aggtgcatgg
     9901 cctgaagacc tcgctggggt gcgcttaccc ggtcacttcg aggacgatct tgcccacatg
     9961 gccggggtcg tcgatgcgct tatgggcgtc ggccgcgtcg ctgagcggaa atgtggagtc
    10021 gatcaccggc ttgaagtcgc ccgccgcgat cagcggccac acggtcttct cgattgcctc
    10081 ggccatgcgc gctttggcct tggcgccttg cgcgcgcagc gtcgagccgg tcagggtgag
    10141 gcggcgcatc atcaaccgca tcaggtcgat ctcgaccttg gagcccttca ggaaggcgat
    10201 ctggacgatg tggccttcga gtgccgcggc ccgcaggttc cgctcgatat agtcgccgcc
    10261 caccatgtcg aggatgacgt cggcgccatg cttctcggtg accttacgga ccacgtcgac
    10321 gaagtcttcc tcgcggtagt tgatggcgcg gacggcgccg agcgcctcgc aggccttggc
    10381 cttggtatcg tcgccaaccg tgacgatgac ttttgcgccg agcgcggtgg ccagctggat
    10441 ggccgtggtg cctatgccgc tggccccgcc atggaccagg agccaatcgc cttcctcgag
    10501 gcccccacgc tcgaagacat tgtgccagac ggtgaagatc gtctccggca gcgcggcagc
    10561 ctgaaccgcg gtcagcccat cgggtgcttt gagcgtggtc gtctccgggg cgacggcaaa
    10621 ctcggcatag ccgccgccat tgaccagcgc gcagacgaag tcgcccggct tgaagcgggt
    10681 gacggcctcg ccgaccatga tcacgggacc ggcgacttcg agcccgagaa gatcggacgc
    10741 gcctttgggc ggcggataaa ggccctggcg ctggagcacg tccggccggt tgatcccggc
    10801 ggcctccacg gcgatcagca cctcgtccgg agcgggcttg ggcgcgggcc gcatggccgg
    10861 caccagaacc tccgggcccc cggcctcgcg cgcttcaatg acagtcatgg tctctggcag
    10921 cattggttcc tcgccgtctc ttcagtccgc gggccatgga taaagggatt atgggcgaaa
    10981 ggccacgatg gtagggtgcg gcatccttta agggacggcc cgcccatgga ccttgacgat
    11041 ctcacgccaa aggcgccgaa aggcgacacg ctcggccaag acctgagcaa gcactccgtg
    11101 gcggaattgg aggcgctgat cgatgcgctc cgggaagaaa tcgcgcgggt ggaagaggcg
    11161 ctgggcgcca agcaatcctc aaaaaacgcc gcggaagccg catttaagcg ctgattaagt
    11221 cctgcacacc gatcgtttag gtaaatcagg ccttagtctt aaccgcttat taggagttgc
    11281 cgcgtaaagt cctcgttatc cagtcctctg gatctgagtg gcctgttcca ctctgttttg
    11341 acgcctccct gttaacttcc tgggccgcct tttttggcgg ctctcttttt gcctgaattc
    11401 ctcgctttaa ccttggttac cagaaggcga cgacaagttc cgcccatggc atactatgcc
    11461 gccccataag gggcgccccg agaaggggct gttgacggag tggggcgaat ggcggggaag
    11521 tccaagaacg acagcaccga tgcttatgag ggcgtaacgg tctcattcgg cgctaaattc
    11581 gcgcgatcgg accagttcaa gactgtcttc cgcgaaggta tggccttggt ggaggccgcg
    11641 gccgcttatc tggacggcga tggccgtaaa gaggcccgca agctcaagcc gccccattcg
    11701 ctggcctatg caacggaaag tatgcgcctg acgacgcggc tgatgcagct ggcgtcttgg
    11761 ctcctgatcc gccgggccgt gagcgaaggc gaactgacgc cggagcaggc ggtcgaggaa
    11821 cagcgcaaga tcaagctccc cgtggctggc gccgagcagc cgacccgcga cttcgaggcg
    11881 ctaccggagc gcctgcgcga tctcatcgag cagagtatca agcttcagga gcgagtggtt
    11941 cgcctcgacc agatgctcga tttcaacgaa cagactgctg agggcgaaac acatcacccg
    12001 ctcagctcgc agctctcggc gctgcacgcg gcattcggct ccgaggccga gtaatccagc
    12061 ctacttcaaa aagccttcgt acttcttctt gaagcgcgtc aggcggccgc cgcgatcgat
    12121 caggtgctgc ccaccgcc
//
LOCUS       DBNHVU010000191         7878 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1182819, whole genome shotgun sequence.
ACCESSION   DBNHVU010000191 DBNHVU010000000
VERSION     DBNHVU010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7878)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7878
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1182819"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..806)
                     /locus_tag="AC16Y7_08135"
     CDS             complement(96..806)
                     /locus_tag="AC16Y7_08135"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR5709387.1"
                     /translation="MSSLKHPPLTIVFDLDGTLVDTAPDLVAATNSILGRVGAPHVSV
                     ERLKMWVGSGARAMIVEGLRNAGGSFLGMSQDGTKVDQLLAEFLDYYEANIAVESRPF
                     DGAEDALIRLRDMGHRLAVCTNKRESLSIKLLDALNLSPYFAAVAGRDTFPVSKPDPG
                     HLTGAVTLANGTRDRAVMIGDSQTDILTAKRADIPVIAVTHGYTDRPVLEFNPTATID
                     HFSEIDAALSALALRHSS"
     gene            1008..2456
                     /locus_tag="AC16Y7_08140"
     CDS             1008..2456
                     /locus_tag="AC16Y7_08140"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HIR5709388.1"
                     /translation="MDQKTDQLIKTQAYINGAWVGSPVTEVLDKASGEVIAKVADCGR
                     VETREAIAAAKAAMPAWAKRTAKERSFLLRKWFDLIVAHQHELALILSREQGKPKAEA
                     LQEIQYGAGFVEFFAEEAKRVYGEIIPSHKADGRILVLKQPIGVVAAITPWNFPNAMI
                     TRKVTPALAAGCTAVVKPAEATPLSALALGALAEEAGLPPGIFNVVTASDPEPVGAEM
                     CENPDVAMVTFTGSTEVGKLLLRQCAGTVKKTSMELGGNAPLIVFDDADLERAIQSTM
                     VSKFRNAGQTCVCANRIYVQRGIHDAFVRRLVEETRKLKVGPGLQEGVTIGPLIEAAA
                     VEKVGAHIVDAVAAGAKVETGGRRHRLGGTFFEPTVLSSVSADMQIARYETFGPVAPV
                     IRFETEDDVIASANATRFGLASYVFTADVGRVWRMAEALEYGIVGVNEGVVSTELAPF
                     GGVKESGLGREGSHYGIEECVEVKYVYLGGLG"
     gene            2589..3320
                     /gene="rpiA"
                     /locus_tag="AC16Y7_08145"
     CDS             2589..3320
                     /gene="rpiA"
                     /locus_tag="AC16Y7_08145"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HIR5709389.1"
                     /translation="MSADALKQAAAEKAMTFIEPGMKLGLGTGSTAAKLVDCLSKAVA
                     GGLDVVCVPTSEATRQQAEAGGIRLTTLDDEPFLDLTIDGADEIDSALRLIKGGGGAL
                     LREKIVAMASERMIVIADSSKKVEQLGAFPLPVEVVPFGLQATLDMMKVLSGDVGCSG
                     ELVVRQKANGEAFRTDSGNLIVDCQFGRIPDPESLDDALKLVPGVVENGLFIAIADLA
                     IIAGDDGISVFENADLADADSASSV"
     gene            3386..4756
                     /gene="gor"
                     /locus_tag="AC16Y7_08150"
     CDS             3386..4756
                     /gene="gor"
                     /locus_tag="AC16Y7_08150"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HIR5709390.1"
                     /translation="MTGFDYDFFVIGGGSGGVRAARIAAGHGAKVAIAEECRFGGTCV
                     IRGCVPKKLLVYASRFRDDFEDAAGFGWQVPGPVFDWPSLIAAKDKEISRLEGVYEKN
                     LRAAGVEIFPERAEFADAHTLRLAASGRQVTADKILIATGGGPSLPAGFDGRRHVITS
                     NEAFDLEALPGHVVIYGGGYIAVEFAGIFNGLGARTTLVYRRDKVLRGFDEDLRDAST
                     EAMQKRGINLVLNANITAVGSNGGSKVVTLDNGEELVADKVMFATGRDPNTKGLGLDR
                     AGVQTKENGAVVVDRSEQTSSPNIYAVGDVTDRVQLTPVAIREGHALADRLFGDGAAS
                     VDYEMIPTAVFSTPEIGTVGFAEHDAVKSFGTVDVYQTAFRPMRATLAGRDERMLMKL
                     LVAQSNGRVVGCHILGDDAGEIVQSVAIAMRMGATKADFDRTMALHPSAAEELVTMRQ
                     KTRVAS"
     gene            4746..5591
                     /locus_tag="AC16Y7_08155"
     CDS             4746..5591
                     /locus_tag="AC16Y7_08155"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5709391.1"
                     /translation="MQANLIGVRTLNGLALRDQLTRQNFRSRGYMKRLEGKVALVTGA
                     GAGIGRAVAKCFAGEGAFVYVSDVNGETAEEVARDILAAGHSAEAMTLDVSRGQDVTH
                     MVRKITQDKARLDVVVNNAGLNVREDFRHLSDDAWEKIREVNLDGVVRIARDTFPLMK
                     AAGSASLINVSSLMGHQPIRQVAAYAATKGAVAALTRALAVEYAAFGIRVNAVAPGFI
                     ETALTERVLKNPFVNKSLLERTPMRRFGTGEDVARAALFFASDDSAYVTGTELLVDGG
                     MAAGS"
     gene            complement(5614..6867)
                     /locus_tag="AC16Y7_08160"
     CDS             complement(5614..6867)
                     /locus_tag="AC16Y7_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007198987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HIR5709392.1"
                     /translation="MKLHQPPAAASRRRESERHSLGFAGNSSEHVARRTMAIREPTFT
                     FGIEEEYHLVDLKTRDFAAVPKELMSALENELGSSVSPEFFRSQIEIGTKVCNSAEEA
                     RADLANLRRQIITHARPHNMAPIAASTHPFAQKTSLETTPKERYQALARDFGGIGRRL
                     AICGMHVHVGIEDDELRIDLMNQVRYFLPHLLVLSTSSPFWEGEDTGLKSYRLAVFHE
                     LPRTGIPQRFESFGEYQRTLDVLINAGVIEDPTKIWWDLRPSGRFPTLELRITDVCTN
                     INDALAIAALYRCLIRMLYRLRRSNQKWRTYPAFLLTENCWRAQRYGTRGSLFDLGKG
                     ELVNFPHLVGELMELVEPDANALGCENDIAHLRTIAERGTSADRQVDRLAVLTAAGKD
                     KAEALKGVVDLLIEETGADVGLTNA"
     gene            7041..>7878
                     /locus_tag="AC16Y7_08165"
     CDS             7041..>7878
                     /locus_tag="AC16Y7_08165"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR5709393.1"
                     /translation="MRSLAEWSPESWRNLPIVQVPDYPDQAVLEDVERKLASFPPLVF
                     AGEARELKAQLADVAAGKGFLMQGGDCAESFLEHGADNIRDFFRVFLQMAVVLTYAGG
                     LPVVKVGRIAGQFAKPRSSPFEKRDGEELPSYRGDIINGAEFTKAARIPDPTRQLEAY
                     RQSAATLNLLRAFASGGYADLERVHQWNLGFVQDSPQGHRYQELADRITECMNFMRAC
                     GVTSETARDLRATRFYTSHEALLLGYEQGLTRRDSTSGDWYATSGHMLWIGDRTRQPD
                     HAH"
BASE COUNT         1515 a         2016 c         2690 g         1657 t
ORIGIN      
        1 aaagagaatt gtgacggctt tgcgcgcggg tggcgttcga attaccggct tttcccgagg
       61 ttgtggccaa aattgaggtt ggggatcgtc cagggtcagc ttgaatgccg aagtgcaagg
      121 gcggacaggg cggcatcaat ctcggagaag tgatcgatcg tggctgtggg gttgaactcc
      181 agaaccggcc ggtcggtata gccgtgcgta acggcaatca ctgggatgtc tgcgcgcttg
      241 gccgtgagga tatcggtctg gctgtcgccg atcatgacgg cgcggtcccg agtgccattg
      301 gcgagggtga cggcgccggt gagatgtcct gggtcgggtt tggagacggg gaaggtgtca
      361 cggcctgcca cagcggcaaa gtaagggctg aggttgagcg catcgagcag cttgatcgag
      421 agggattcgc gcttgttggt gcagaccgcg aggcggtggc ccatgtctcg caggcgtatc
      481 aaggcgtctt cggcgccgtc gaaggggcgg ctctcaacgg cgatgttggc ttcgtagtag
      541 tcgaggaact cagccaacag ctggtcgacc ttggtgccgt cttgtgacat gccaaggaag
      601 gatccgccgg cgttcctcaa gccttcaacg atcatggcgc gggcgcctga cccgacccac
      661 atcttcaggc gctcgaccga gacgtgaggg gcgccgacgc gtccaaggat cgaattggtg
      721 gcagcgacca gatcgggcgc ggtgtcgacc agtgttccat caaggtcaaa aacgattgtg
      781 agcggcgggt gcttcaggga ggacatggcg tcggtttacc ggctctttgg cgcagcttca
      841 atgggccatt gcagagccga tccggtgtct tgcagcggcg tgttgacccg catttgccag
      901 ccgtcgcaca gtgctggacc agcttgagcg tgacttcata atgagtggca gggcggcacg
      961 cgccgtagct acgttctgag ggtgcgggat tggatatgtg agaggacatg gatcagaaga
     1021 cagatcaact tatcaagacg caggcctaca tcaacggcgc ctgggtcggc tcgccagtca
     1081 cggaagtctt ggacaaagct tcgggagagg tgatcgccaa agtggccgat tgcggccggg
     1141 tggagacacg cgaggcgatt gccgcagcca aagcggcaat gccggcgtgg gcgaagcgga
     1201 cggcaaagga gcggtctttc ctgttgcgga aatggttcga tctgattgtt gcccaccaac
     1261 acgaacttgc gctcattttg tcgcgcgagc aaggcaagcc caaggccgag gcgcttcagg
     1321 aaattcaata tggcgccggg tttgtggagt tctttgccga agaggcaaag cgcgtttatg
     1381 gcgaaatcat tccgtcgcac aaggcagatg ggcggatact ggttctcaaa cagccgatcg
     1441 gtgtcgtggc ggcgatcacg ccgtggaatt ttccaaatgc catgatcacg cgcaaagtta
     1501 cgccagcgtt ggcggcgggc tgtacggcgg ttgttaaacc ggctgaggcg acaccactgt
     1561 cggccttggc gcttggtgca ttggctgagg aagcggggct gccgcccggt attttcaacg
     1621 tcgtgaccgc gagcgatccc gagccggtcg gggcagagat gtgcgagaac ccggacgtgg
     1681 cgatggtgac gttcaccggt tcaacggagg tcggcaagct attgctgcgc cagtgtgccg
     1741 ggacggtcaa gaagacttcg atggagcttg gcggcaatgc gccgctgatt gtcttcgacg
     1801 atgccgatct agagcgggcc atccagtcaa cgatggtgag caaatttcgc aacgccggtc
     1861 agacctgcgt ctgcgccaat cgcatttatg ttcagcgcgg tatccacgat gccttcgtgc
     1921 gccgcctcgt tgaggaaacc aggaagctga aggttgggcc gggcttgcag gaaggtgtga
     1981 cgatcggtcc gctgattgag gcggctgcgg ttgagaaggt aggagcgcac atcgtggatg
     2041 ctgtggcggc gggcgccaag gttgagaccg gcgggcggcg tcacaggctg ggtggcacat
     2101 ttttcgagcc gacagtcctg agttccgtct cggctgacat gcagattgcc cgatacgaaa
     2161 ctttcgggcc ggttgcgcct gttatccgct ttgaaacgga agacgatgtg attgcaagcg
     2221 ccaatgcgac acggttcggg ctcgcgtcct atgttttcac ggccgatgtt ggccgcgtct
     2281 ggcggatggc ggaggccctg gaatacggca tcgtgggcgt caatgagggt gtggtttcga
     2341 cggagcttgc gccctttggc ggcgtcaagg aaagcggact cgggcgtgag ggctctcact
     2401 atggcatcga ggaatgcgtt gaagtgaaat acgtttacct cggtggtctc ggttagattg
     2461 gttcgggcct ggatgtttca atcgacgatt tgacgtggga ttgccatgcg ccccgcggtc
     2521 atcctagttt gtcggcgatt ggatgcgcgc cctttggctg cgtcagaccc ctcaccaagg
     2581 agagagccat gagtgcggac gccctcaagc aggcggcagc ggaaaaggcc atgaccttta
     2641 tcgagccggg catgaagctt ggcctgggca caggctcaac ggcggccaag ctggtcgatt
     2701 gtctctcgaa ggcagtcgcc ggcgggctcg acgtggtgtg tgtgccaacg tctgaagcaa
     2761 cccgccagca ggccgaggcg ggtggcatcc gcctgacgac acttgatgac gagccgtttc
     2821 tcgatctgac gatcgatggg gccgacgaaa tcgattccgc gctgcgtctc atcaaaggcg
     2881 gcggcggggc gctgttgcgc gaaaagatcg tcgccatggc gtccgagcgc atgatcgtaa
     2941 ttgccgatag cagcaagaag gtggaacaac tcggggcgtt tccgttgccc gtcgaggttg
     3001 tgccgttcgg gctccaggcg acgctcgaca tgatgaaggt gttgagtggg gatgtcgggt
     3061 gttcgggaga gcttgtggtc cgccaaaagg ctaacggcga agcgttccgt accgatagcg
     3121 gtaatttgat tgtcgattgc cagttcgggc ggatcccgga ccctgaatcg ctggacgatg
     3181 ccttgaagct ggtgccgggc gttgttgaga acggcctttt tatcgccatt gccgatctcg
     3241 cgatcatcgc aggcgacgac ggtatcagcg tttttgagaa cgccgatctt gccgatgccg
     3301 attcagcgtc atccgtttaa cgaaggtctg cggtttttta caaagatgcg ctgctttaag
     3361 tttgcacaag gcgaattcga aagtgatgac ggggtttgac tacgattttt ttgtgattgg
     3421 cggtggctcg gggggcgtgc gggcagcccg catcgctgcc ggccacggcg ccaaggttgc
     3481 catcgctgag gaatgccggt ttggcggcac gtgtgtcatt cgaggctgcg tgccaaaaaa
     3541 gttgctcgtc tacgccagcc gttttcgcga tgactttgag gatgcagcgg gatttggctg
     3601 gcaggtaccg ggtcctgttt tcgactggcc gtcactgatt gcagccaagg acaaggaaat
     3661 ttcccggctc gaaggtgtct atgagaaaaa cctccgggcg gcgggtgtcg aaatttttcc
     3721 ggaacgtgct gagttcgctg atgcgcacac gcttcgcctt gccgcttcgg gccgccaggt
     3781 cacggctgac aagatcttga ttgcaaccgg gggtgggcca agcctgccgg ccggttttga
     3841 cggacgccgg cacgttatca cctcgaacga ggcatttgat cttgaggcac tgcccggtca
     3901 cgtcgttatt tacgggggcg gctatatcgc cgttgagttt gctggaatct ttaatggatt
     3961 aggcgccagg acaacgctgg tttaccgccg ggacaaggtt ctgcgcgggt ttgatgaaga
     4021 tttacgcgac gcttcgacgg aggcgatgca aaagcgcggc atcaaccttg ttctcaacgc
     4081 caacatcact gctgtcggca gcaatggcgg ctcgaaggtg gtgacgctag ataatgggga
     4141 agagctggtc gctgacaagg tgatgtttgc gacgggccgt gatcccaaca ccaaaggtct
     4201 cggcctcgac cgtgcgggcg ttcaaacaaa agaaaacggt gctgtcgttg tcgaccggag
     4261 cgaacaaaca tcaagcccca atatctatgc tgtgggcgat gtgacggatc gagtgcagct
     4321 gacgccggtg gccatccgcg aggggcatgc tcttgcggat cgtctgtttg gagacggggc
     4381 ggcgagcgtc gactatgaaa tgatcccgac ggcggttttt tcaactccgg aaatcggcac
     4441 tgtcggtttt gccgaacacg atgccgtgaa atcgttcggg actgttgatg tttatcaaac
     4501 agctttccgg ccgatgcggg cgacgctggc cgggcgcgat gagcggatgc tcatgaagct
     4561 tctcgtcgcg cagtcgaatg gccgcgttgt tgggtgtcat atcctcggtg acgatgcagg
     4621 ggaaatcgtg caatcggtag caatcgcgat gcggatgggg gcgaccaagg ctgacttcga
     4681 ccggacgatg gcgttgcacc cgagtgctgc ggaggagctt gtgacgatgc ggcagaagac
     4741 cagggttgca agctaatctc attggcgttc gaacattgaa cggcttggcg ttgcgtgatc
     4801 agttgacgcg gcaaaatttc cgatcaagag gatacatgaa gcgtttagag ggaaaagtcg
     4861 cactcgttac cggcgccgga gcgggcatag ggcgcgccgt cgccaagtgt ttcgcaggcg
     4921 aaggggcttt cgtttatgtg tcggacgtca acggcgagac agcggaagag gtggcgcgtg
     4981 acatattagc ggctgggcac tccgccgaag cgatgacgct tgatgtgtca cgcgggcagg
     5041 atgtcacgca catggtgcgc aagatcaccc aagacaaggc caggcttgat gtcgtcgtca
     5101 acaatgccgg gctgaatgtg cgcgaggact tccgtcactt gagcgatgac gcgtgggaga
     5161 agattcgcga ggttaatctt gacggggtgg tgcggatagc tcgcgatacg tttccgctaa
     5221 tgaaggcggc gggttctgcg tcgttgatca atgtctcgtc cttgatgggg catcagccga
     5281 tccgccaggt agcggcttat gcagcgacga aaggtgcggt tgcggcgctg acaagggcgt
     5341 tggcggtgga atacgcggcc tttggcattc gggtgaacgc ggtggcgccg gggttcattg
     5401 aaacggctct cacagaacgc gtcctcaaga atccattcgt caataagtcg cttttggagc
     5461 ggactccgat gcgccggttt gggacggggg aagatgtggc acgggcggcg ctgttctttg
     5521 cttctgacga ttccgcttac gtaacgggaa cggagttgct ggttgatggc ggcatggcag
     5581 cgggaagcta gccaggcggc tccgggggcg ggcttaggcg ttggttaggc cgacatcggc
     5641 tccggtttct tcgatcaaaa ggtcgacaac ccctttcagg gcttctgctt tgtccttgcc
     5701 agcggcggtc aagacggcga gccggtcaac ctggcggtcg gcgctggtgc cgcgttccgc
     5761 gatggtgcgc agatgcgcga tatcattttc gcagccaagc gcgttcgcgt ccggttcgac
     5821 gagttccatc agctctccga cgagatgcgg gaaattcacc aactcgccct tgccgagatc
     5881 gaacagggag ccgcgtgtgc cgtaacgctg tgcccgccag cagttctccg tcagcaaaaa
     5941 tgcggggtag gtccgccact tctgattgga acggcgaagg cgatacagca tgcgaataag
     6001 gcagcggtag agggcggcga tggcgagagc gtcattgatg ttggtgcaga catctgtgat
     6061 gcgcaattcg agtgtcggaa aacggccgga cgggcgcagg tcccaccaga tttttgtcgg
     6121 gtcttcaatg acgccagcgt tgatcagcac gtcgagagtg cgttgatact cgccgaaact
     6181 ttcaaagcgc tgggggatgc cggtacgtgg caattcgtga aaaaccgcaa ggcggtagct
     6241 tttgaggccg gtatcctcgc cttcccagaa cggcgatgag gtcgagagca caaggagatg
     6301 aggcaagaag tagcggacct ggttcatcag gtcgatcctg agttcatcat cctcgatgcc
     6361 gacgtgaacg tgcatgccgc agatggcgag gcggcggccg atgccgccga agtcacgggc
     6421 aagcgcctgg tagcgctctt tcggtgttgt ctcgagggag gtcttctgtg cgaatggatg
     6481 agtcgaggcg gcaatcggtg ccatgttgtg ggggcgggcg tgggttatga tctggcggcg
     6541 cagattggca aggtcggcgc gggcttcctc agcgctatta cagactttgg tgcctatttc
     6601 gatttgcgag cggaagaact cagggctgac ggaactgccg agttcattct ccagagctga
     6661 catgagctct tttgggacgg ctgcgaaatc gcgcgtcttg agatcgacga ggtggtattc
     6721 ctcttcgatg ccgaaggtga aagtgggctc gcgaatggcc atggttctcc ttgccacgtg
     6781 ttctgaagag tttccagcaa aaccaaggct gtggcgctcg ctctcgcgtc tccggctggc
     6841 ggcggcagga ggctgatgca acttcacgat gatgggtggc gcggtgccaa gtcatggcct
     6901 ataagcggct caaagcgtgg tataaggaaa actcgtggcc ctcgcggtca tggttaatgt
     6961 ccgcgctccg cttcgcctgt gctttggcga gaagaggcct aacggcaaaa gtagaatagg
     7021 agtacatggc gatggcagcc atgagatcgc ttgcagagtg gtcgcccgaa agctggcgta
     7081 accttccgat cgtgcaagtg ccggattatc ccgaccaggc tgtccttgag gacgtcgaga
     7141 ggaagcttgc gagcttcccg ccgctggtgt tcgcgggcga ggcgcgcgaa ttgaaagccc
     7201 agcttgcgga cgtcgcagcc ggcaagggtt tcttgatgca aggcggcgat tgcgccgaga
     7261 gcttccttga gcatggcgcg gacaatatcc gcgacttctt ccgcgtcttc ctgcagatgg
     7321 cggtcgttct aacgtatgct ggcggtttgc cggtggtgaa agtcgggcgg atcgcggggc
     7381 agtttgcgaa gccgcgttcg tcgcctttcg agaagcgcga cggcgaggag ttgccgagct
     7441 atcgcggcga catcatcaat ggtgcggagt ttacgaaagc ggcgcggatt cccgatccga
     7501 cgcgccaact ggaagcttac cgtcagtcgg ctgcgacttt gaaccttctg cgcgcgttcg
     7561 cgtccggcgg ctatgccgac ctcgagcgcg tgcatcagtg gaacctgggc ttcgtacagg
     7621 acagcccgca gggtcaccgc tatcaggaac ttgccgatcg tattaccgag tgcatgaatt
     7681 tcatgcgcgc ctgcggtgta acgtctgaga cggcgcgcga ccttcgggca acgcgcttct
     7741 acactagcca tgaggcgctg ctgctcggct atgagcaggg gctgacacgg cgcgattcaa
     7801 cgagtggcga ttggtacgcg acatccgggc atatgctttg gatcggcgac aggacgcgcc
     7861 agccggacca tgcgcatg
//
LOCUS       DBNHVU010000192         7803 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1928011, whole genome shotgun sequence.
ACCESSION   DBNHVU010000192 DBNHVU010000000
VERSION     DBNHVU010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 7803)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7803)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7803
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1928011"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..96
                     /locus_tag="AC16Y7_08170"
     CDS             <1..96
                     /locus_tag="AC16Y7_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007532219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR5709394.1"
                     /translation="GSVSDDEAKTIYPNGWKAPRPYIRIVPQPKG"
     gene            497..949
                     /locus_tag="AC16Y7_08175"
     CDS             497..949
                     /locus_tag="AC16Y7_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR5709395.1"
                     /translation="MGFPKTPAVTVDCVIFDSQSRVLLIRRKNDPFKGAFALPGGFVD
                     LGETVEAGCRREVHEETGLDVGDLQLVGVYSDPHRDPRGHTVSMAFLAKLPAAPAPVA
                     GDDAASAAWVGDWRAHTLAFDHAQILVDAEKLAERGAPGAAQGTPQEG"
     gene            1005..2057
                     /gene="hemH"
                     /locus_tag="AC16Y7_08180"
     CDS             1005..2057
                     /gene="hemH"
                     /locus_tag="AC16Y7_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HIR5709396.1"
                     /translation="MNEAPRTEAFSDTFDCRSVAYGKIGVLLVNLGTPDDTDYWSMRR
                     YLKEFLSDRRVIEVNRLLWWPILNGIVLTTRPSKSGEAYRSIWNEELDESPLRTVTRA
                     QAEKVADTLGHREEIVVDWAMRYGTPSIASGVEALIKQGCEKLLVFPLYPQYAAATTA
                     TVNDTVFDKLKTMRWQPALRTVPPYPKDPVYIEALANSITAHLADLDFEPEVILASYH
                     GLPKEYVDKGDPYQCQCMDTSDALREALGPLGDALKTTFQSRFGRAEWLQPYTDKTVE
                     ALARDGVKRMVILTPGFAADCVETLEEIDGEAREIFLEHGGEQFSSIPCLNDSDDGIR
                     VLVHLIERELKGWIDE"
     gene            2071..2520
                     /locus_tag="AC16Y7_08185"
     CDS             2071..2520
                     /locus_tag="AC16Y7_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709397.1"
                     /translation="MIWNAFSVLGICVGLLVAIPAHGAGELAPDELAAYVDVVGAPGD
                     GKEALERALSNRLLDRGFTVTGSPAINAYEIQGIVSLSPAARGKENIRINWIVFGPDG
                     TRLSNVTQSRVIRKGSLSRRWGPAADAAAEAAAQDIFKLLPPRDSGL"
     gene            2625..3596
                     /locus_tag="AC16Y7_08190"
     CDS             2625..3596
                     /locus_tag="AC16Y7_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR5709398.1"
                     /translation="MFGFDIFVLALLVLFVIIVIAAVKIVPQGYNYTVERFGRYTRTL
                     VPGLSLIIPFIDRIGRKLNMMEQVTNVPSQEVITRDNAMVRVDGVAFFQVLDAPRASY
                     EVTDLERAILNLTMTNIRTVMGSMDLDKLLSERDEINHRLLKVVDDATHTWGVKVTRI
                     EIKDIEPPRDLVDAMARQMKAERDKRAAILEAEGERQSEILSAEGSKQAQILAAEGRR
                     EAAYRDAEAREREAEAEAKATHMVSQAINKGSVQAINYFVANNYVDALKELAAADNQK
                     VLLMPLEASSVIGAIAGVSEIAREAFGDSKKESKPRGGRSGGSVPSA"
     gene            3612..4061
                     /locus_tag="AC16Y7_08195"
     CDS             3612..4061
                     /locus_tag="AC16Y7_08195"
                     /inference="COORDINATES: protein motif:HMM:NF014062.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR5709399.1"
                     /translation="MMETLAELGAWSWMIAAAILFVLELVAPGIFFMWFGAAALATAL
                     IVFRYDITWQWQLIWFCGLSLASILLVGRYLRGNPLKSDKPLLNKRATQLVGQSYELV
                     DPIENGRGSVHVGDTIWRVKGPELPKGARVKVVGTDGSVLTVEADEA"
     gene            complement(4063..4296)
                     /locus_tag="AC16Y7_08200"
     CDS             complement(4063..4296)
                     /locus_tag="AC16Y7_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709400.1"
                     /translation="MPQFTVEKGKRYRATLQLSLLQSIATNEMVADEFRKAGFTDVAV
                     EGSGSTRYATGLWSQDNATAEMPSEVSEITTIA"
     gene            complement(4308..5807)
                     /gene="glpD"
                     /locus_tag="AC16Y7_08205"
     CDS             complement(4308..5807)
                     /gene="glpD"
                     /locus_tag="AC16Y7_08205"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HIR5709401.1"
                     /translation="MGGSSFDLLVVGGGIHGAAVARDAAGRGLKVILAEKGDYACATS
                     SASSKLIHGGLRYLEHVEVGLVRESLTERAELLRTAPHLVAPLKFLLPIYSWQERKAW
                     FVHAGLTLYDLLSFGDGLPASGRVSAAETAHLPRLRQEDLRAVLHYHDCQTDDARLTL
                     SVLLDARSRGADILNRRTVTAIAAAEDGYTVELDERGTKRQITARFIVNAAGPFVPNI
                     DAMTEAAPPPQALRLVRGSHIVLRMPDPPEPCAYTLQDQGERIVFVIPWIDGRFLIVG
                     TTDVPHEGDPGTAECSPDEKSYLVHAYNRYFTTPGRTVTERDIVFTWSGVRALHGGTE
                     TKPSRISRSPTLVACAQGSGGFLTIYGGKLTTHRALAEDVLANLETLGLSMSGSWTKN
                     VPLYGGKLDRAALEAKAEAGPDSVPAATRHRWAFTYGDRIEDLFARLKADRASAKDVA
                     PGVLEAELEYAAEAEDAMTADDFLLRRTKLHLLLDEPGRNAVREWFAGT"
     gene            complement(5820..6827)
                     /locus_tag="AC16Y7_08210"
     CDS             complement(5820..6827)
                     /locus_tag="AC16Y7_08210"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HIR5709402.1"
                     /translation="MSAGLKQDLSHTENSSEAVASATRTLQLEAEGLSALQAALTAHL
                     AENFTQALDILRGCRGRVIVTGMGKSGHVGLKVAATLSSTGTPALFVHPSEASHGDLG
                     MITRDDALLAFSWSGETVELGNILSYSRRFAVPLIAITSKPDSTLSQAAEVVLALPQA
                     KEACPHGLAPTTSTMMQLALGDSIAIALLESKGFTALDFGVLHPGGQLGARLKFVGDL
                     MHHGDHLPLTSVDTVMSEAIVVMTEKALGCLGVVDNDGVLKGIITDGDLRRHMGDGLL
                     VARAADIMTPGPKTVTPDLMASAALEVINSSSITALFVVENGRPVGIIHIHDLLRAGV
                     V"
     gene            7484..>7803
                     /locus_tag="AC16Y7_08215"
     CDS             7484..>7803
                     /locus_tag="AC16Y7_08215"
                     /inference="COORDINATES: protein motif:HMM:NF021589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR5709403.1"
                     /translation="MGAPRASDNVLDNRPVLPERTRLDPYMPIGMKLGSFLLYAETEV
                     GTILTDNVLGTPIPRSDYALEFAPNLVLQSNWTRHSFTASFEADRSWYKEFPVEDTKT
                     YAAAL"
BASE COUNT         1420 a         2399 c         2508 g         1476 t
ORIGIN      
        1 ggttctgtat ccgacgacga ggccaagacc atctacccga atggctggaa ggcgccgcgg
       61 ccttacattc ggatcgttcc acaacctaag ggataggttg aaactccgag gataagtcat
      121 cggtttagat tggaagcacc acgccacttc gtgtaaaagg gatgcatacg gagtaaccgg
      181 ttcgtagcca aattttagga acgaagtgtc ggtgccttcg gggccggccg gatcaccggg
      241 cgaaatcgat gtctgacttt catgacctac ctccgcctgg agcaggggag gcttcgcgtg
      301 agcgtgcgaa gaagatggcc gatcagatcg tgcttctcat gacggggcgc ggtccgaacg
      361 gttcgacttc ctcgcctatc tcctggccat ggcgctgaga gaagtcaggc ggctggtgca
      421 ggacacgcgc taggcccacg ccgcatagac agcgctacaa gacttcacca cagattctcg
      481 ccgagatttc attgccatgg gatttccgaa gacccctgcg gtgaccgtcg attgcgtcat
      541 cttcgattca caatcgcgcg tcctcctcat ccgccgcaag aacgatccgt tcaagggcgc
      601 gttcgcgttg cccggcgggt ttgtcgatct cggcgagacc gtcgaggcgg ggtgccgccg
      661 cgaagtgcat gaggaaacgg gcctggatgt cggggatctc cagctggtcg gcgtctattc
      721 cgatccgcat cgcgaccccc gtggacatac ggtgtctatg gcctttctgg caaagctccc
      781 cgccgccccg gcgccggtgg ccggcgacga tgccgcctcc gcggcctggg ttggcgattg
      841 gcgggcgcac acgcttgcct tcgaccacgc tcaaattctg gtagatgccg agaaattggc
      901 ggaacgcggc gctccaggtg cggctcaagg tacgcctcaa gaaggctagg cggatccggc
      961 ccgatggcct atctaagggg ccagggctct ttaggatgcc gcaaatgaac gaagcgccgc
     1021 gaaccgaagc tttctccgac acctttgact gtcgctcggt tgcctacgga aagatcggcg
     1081 tgctcttggt caatctcggc acgccggacg acaccgacta ttggtcgatg cgccgctatc
     1141 tcaaggagtt cctgtccgac cgccgcgtca tcgaggtcaa tcggctcctg tggtggccta
     1201 tcttgaacgg catcgtgctc accacacgtc cgtcgaaaag cggcgaggcc tatcgctcca
     1261 tctggaacga ggaactcgac gagtctccgt tacgcaccgt cacgcgcgcg caagccgaga
     1321 aggttgcgga cacgcttggc caccgcgagg agatcgtcgt cgattgggcg atgcgctacg
     1381 gcacgcccag cattgcctcc ggggtggaag cgctgatcaa gcagggctgc gaaaagctgc
     1441 tcgtgtttcc gctctatccg caatacgccg cggcgaccac ggcgacagtc aacgacacgg
     1501 tcttcgacaa gctcaagacc atgcgctggc agccggcgct gcgcacggtg ccgccatacc
     1561 ccaaggaccc cgtctatatc gaggcgcttg cgaattcgat cacggcgcat ctggccgatc
     1621 tcgacttcga gcccgaggtg atccttgcct cgtatcacgg cctgccgaag gaatacgtgg
     1681 acaaaggcga tccttatcaa tgccaatgca tggatacgtc ggatgctttg cgggaggcat
     1741 tgggaccact tggcgacgcg ctgaagacga cgttccagtc acgatttggt cgtgcggagt
     1801 ggctacagcc ttacaccgac aagaccgtgg aggcgctggc gcgcgatggg gtgaagcgca
     1861 tggtcatttt gacgcctggc ttcgcggccg attgcgtgga gactctggaa gagattgacg
     1921 gcgaggctcg ggaaattttt ctggagcatg gcggcgaaca gttcagctcc attccgtgtc
     1981 tgaatgacag cgatgacggt attcgcgtcc tggtccacct catcgagaga gaactcaaag
     2041 gctggatcga cgagtaggag tgcacggggg atgatctgga acgcgttttc agtgcttggc
     2101 atttgtgttg gcttgctcgt ggcaataccc gctcatggtg ccggtgaatt ggcccccgac
     2161 gaacttgccg cctatgtcga cgtggtcggc gcgccgggcg acggcaaaga ggcgctggag
     2221 cgggcgctgt ccaaccgtct gctcgaccgg ggtttcacgg taaccggcag ccccgccatc
     2281 aatgcgtatg agatccaagg catcgtcagc ctctcgcctg cggcccgggg caaggagaat
     2341 atccggatca attggatcgt attcggcccc gacggcaccc ggcttagcaa tgtgacccag
     2401 tcccgggtta tccgcaaagg ctcgctcagc cgccgctggg ggcctgccgc ggatgccgcc
     2461 gcggaggcgg ccgcccagga tatcttcaag ctccttcctc cacgagattc ggggctgtag
     2521 aaccgttcgt ttgaactccc gcgggccgta aatccggtat agaactcgcc catctgttga
     2581 ttgaagaccc ggcgccgccg ccggatacca cgggagcgtg ccttatgttc ggcttcgata
     2641 tcttcgtcct cgccctgctt gttctcttcg tgattatcgt catcgcggcg gtcaagatcg
     2701 tgccgcaagg ctacaactac acggtcgaac gcttcggccg gtacacacga acgctcgttc
     2761 cgggcctcag tctgatcatc cccttcatcg atcgtatcgg gcgcaagctc aatatgatgg
     2821 agcaggtgac caacgtgccg agccaggagg tgatcacccg cgacaacgcc atggtgcggg
     2881 tcgacggcgt ggccttcttc caagtgctcg atgcgccgcg cgccagctac gaggtgaccg
     2941 atttggagcg cgccatcctc aacctcacca tgacgaatat ccgcacggtg atgggctcca
     3001 tggatttgga caagcttctg tccgaacgcg acgagatcaa ccatcgcttg ctcaaagtgg
     3061 tcgacgacgc cacccacact tggggcgtta aagtcacgcg catcgagatc aaggatatcg
     3121 agccaccgcg cgacctggtc gacgccatgg cgcggcagat gaaggccgag cgcgacaagc
     3181 gtgccgccat ccttgaggcc gaaggcgagc ggcagtcgga gatcctgtcc gcggagggtt
     3241 ccaagcaggc gcagattttg gccgccgaag gccgccgcga agcggcctat cgggatgccg
     3301 aggcccgcga gcgcgaggcc gaggccgaag ccaaggccac tcacatggtc agtcaggcca
     3361 tcaacaaggg cagcgtccag gcgatcaatt actttgtcgc caacaactat gtggatgcgc
     3421 tgaaagagct ggccgcggcc gacaaccaga aggtgctcct catgccgctc gaggcctcgt
     3481 ccgtgatcgg cgcgattgcc ggcgtcagcg agatcgctcg tgaggcgttc ggcgactcaa
     3541 aaaaggaatc gaagccgcgc ggtggacgct ccggcgggtc ggttccctcg gcctaacgcg
     3601 aaggacaaga gatgatggag acgctcgctg agcttggtgc ttggagctgg atgatcgcag
     3661 cagcaattct gtttgtgctg gagttggtcg cgccgggtat cttctttatg tggttcggtg
     3721 ctgccgcctt ggcgaccgcg ctgatcgtct tccgctacga catcacctgg cagtggcagc
     3781 tcatctggtt ttgcggtttg tcgctcgcct cgatcctgct ggtcggccgc tatttacgtg
     3841 gcaatccgct taagagcgac aaacctttgc tcaacaagcg cgccacgcag ctggtcggcc
     3901 agtcgtacga gcttgtcgac cctatcgaga acggacgcgg cagcgtccat gtcggcgata
     3961 cgatttggcg ggtgaagggg ccggaattgc ccaagggtgc gcgcgtcaaa gtggtcggta
     4021 ccgacggctc ggttctaacg gtcgaagccg acgaggcttg agctacgcga tcgttgtgat
     4081 ctcggagacc tcgctgggca tctcagcggt ggcattgtcc tgggaccata agcctgttgc
     4141 gtatcgagtg ctgcccgagc cttcgaccgc cacatcggtg aagccagcct tacggaactc
     4201 gtcggcaacc atttcgttgg tcgcgatgct ctggaggagg ctgagctgga gggttgcgcg
     4261 gtagcgtttt cccttttcga cggtgaactg cggcatgtct tctccactca ggttccagcg
     4321 aaccattctc tgaccgcgtt acggcccggt tcgtcaagca gcaaatgaag tttggtgcgg
     4381 cggagcagaa agtcgtccgc cgtcatggcg tcctctgctt cggctgcata ttcaagctcg
     4441 gcctcaagaa cgccgggcgc gacgtccttg gccgatgcgc ggtcggcttt gaggcgcgcg
     4501 aacagatcct cgatccggtc accataggtg aaagcccagc ggtggcgtgt cgcggccgga
     4561 acactgtcgg gccccgcctc ggcctttgcc tccaaggccg cgcggtcgag cttgccgcca
     4621 taaagcggca cattcttggt ccaagagccg ctcatgctga ggcccaacgt ctccaggttt
     4681 gcgagcacgt cttcagcgag agcgcgatgc gtggtgagct tgccgccata gatcgtgagg
     4741 aagccgccgc tgccttgggc gcacgcgacc aatgtggggc tccggctgat gcggctcggc
     4801 ttcgtctccg tcccgccatg cagtgctcgc acgcccgacc aggtgaagac gatatccctc
     4861 tcggtgacgg tgcggcccgg cgtggtgaaa tagcgattat aggcgtggac gagataggac
     4921 ttctcatccg gcgagcattc ggcggtgccg ggatcgccct catgggggac atccgtcgtg
     4981 ccgacgatga ggaagcggcc gtcgatccag ggaataacga agacgatgcg ttcgccctgg
     5041 tcttgcagcg tataggcgca aggctccggc gggtccggca tccgaagtac gatgtggctg
     5101 ccgcgcacca ggcgcaaagc ttgtggcggc ggggcggcct ccgtcatggc atcgatgttg
     5161 gggacgaagg ggccggccgc gttgacgatg aagcgcgccg tgatctggcg cttcgtcccg
     5221 cgttcatcga gctccacggt gtagccgtct tccgccgccg cgatcgccgt cacggtgcgg
     5281 cggttgagaa tatcggcacc ccggctgcgc gcatcgagga gaacgctcaa cgtgagccgc
     5341 gcgtcgtcgg tctggcagtc gtgatagtgc agcaccgcgc gtaagtcttc ttggcgcagg
     5401 cgaggtaggt gtgcggtctc tgctgccgag acccggccgc tggccgggag cccatcgcca
     5461 aacgacagaa gatcgtagag ggttagacct gcgtgcacga accaagcttt gcgttcctgc
     5521 cagctataga tgggcagcaa gaactttagc ggcgcgacga gatgcggtgc ggtgcgcaag
     5581 agctcggcac gttcggtgag agattcgcgc accaggccga cttccacgtg ctcaagataa
     5641 cgcaggccgc catggatgag cttcgacgac gctgacgaag tggcgcacgc atagtcgccc
     5701 ttctcggcga ggattacctt gaggccgcgt ccggccgcat cacgtgcgac cgcagcgccg
     5761 tgaatcccgc cgccgacaac aaggagatcg aagcttgagc cgcccacccg cgcggggtcc
     5821 tacaccacgc cggcgcgcag caggtcgtgg atgtggatga tgccgacagg tcgtccgttc
     5881 tcgaccacga acaacgccgt aatcgacgag gaattgatga cctcgagtgc ggcggaggcc
     5941 atgaggtcgg gcgtgaccgt cttcggtccg ggcgtcatga tgtcggccgc gcgggcgacg
     6001 agcaggccat cgcccatgtg acggcgcaag tcgccgtccg tgatgatgcc tttgaggacg
     6061 ccgtcgttgt cgacgacgcc gaggcagccg agcgcttttt cggtcatgac gacgatcgcc
     6121 tctgacatga cggtgtctac ggacgtcagg ggaagatgat cgccgtggtg catcaggtcg
     6181 ccgacgaatt tcagccgtgc cccgagctga ccgccgggat gcaaaacgcc gaagtccagc
     6241 gccgtgaagc ccttgctctc cagcagcgcg atggcgatgg agtcgccgag ggcgagctgc
     6301 atcatggtcg acgtggtggg tgccagccca tgcggacagg cctccttggc ttgtggcaag
     6361 gcgagaacca cctcggccgc ttgggacagc gtggagtctg gtttcgacgt gatggcgata
     6421 agcggcacgg cgaaacgccg cgagtaagat agaatgttgc caagctccac ggtctcgccg
     6481 gaccaggaga aggcgaggag ggcatcgtcc cttgtgatca tgcctagatc gccatggctc
     6541 gcttcgctcg gatgcacgaa aagggcaggc gtgcccgtgg aggagagggt ggcggcgacc
     6601 ttcaagccga catggccgct cttgcccatg ccggtgacga tgacccggcc gcggcagccg
     6661 cgcaggatat cgagggcctg ggtgaagttc tcggcgagat gcgccgtcag agccgcttga
     6721 agcgcgctca agccctcggc ctcgagttgc agggtccgcg tggccgaggc cacggcttcg
     6781 ctgctgttct cggtgtgcga gagatcctgc ttcaacccgg ctgacatgtg atttttctcc
     6841 cctcgaggcg tcgattttag cacggaatgc ggcgacgaaa ggtccaagcc gcgcgccaag
     6901 gccccgcgcc atttcgaaac acctctaaac ggcccgttaa ccctcttgct cctaggcttg
     6961 agtatccacg ggtgttggtg ccgacctcgc gatttttcgc ggattctagg cctttcaccg
     7021 agtttttcga tcgaggtgcg tccttgccgc gtcgccacac gtccagcgca gcgggcgcgc
     7081 tcgctgcagc cttactcgcc gctctttgtg cgccggccgc cgctcaagat gcgtcggttc
     7141 cccggggccc agcttccggc cgctccacct ttgcgcggca gcatcggcgc gcagagtggt
     7201 tctgaggcga cgccgcccct ctctcgtacc aggcagcgcc gggaggccga gcggaacgcc
     7261 gtgaacagga gcgtcacgtc cgatggcgtc tttaacaccc agacacccgg caatctggcc
     7321 aacgacccag ccttgacccc cgcgccgccg gccttgcccg cgctgccggc cgcacctacc
     7381 ccgccgcccc ccgctcgggg gcctgggcga tggagacgat caggcagtcg acgtggctcg
     7441 ggaacagggc cctgggatca ccatgccggc cggaacgcct ttgatgggag cgccacgcgc
     7501 gtccgacaat gttctggaca accggccggt tctgccggag cgaactcggc ttgaccctta
     7561 catgcccatc gggatgaagc ttggcagttt cctgctctac gccgagactg aggtcggcac
     7621 gatcctgacc gacaacgtgc tgggaacgcc tattccgcgc tcggactatg ccttagagtt
     7681 tgcgccgaac ctcgtgctgc aatcgaattg gacccggcac tccttcaccg cctcgttcga
     7741 ggccgaccgt agttggtaca aggagttccc cgtcgaggac accaagactt acgcggcggc
     7801 tct
//
LOCUS       DBNHVU010000193         5889 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1110765, whole genome shotgun sequence.
ACCESSION   DBNHVU010000193 DBNHVU010000000
VERSION     DBNHVU010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5889)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1110765"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="AC16Y7_08220"
     CDS             <1..1169
                     /locus_tag="AC16Y7_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5709404.1"
                     /translation="INAHTVDFVTKGPYPLVLNLATYIFPMDREFYSGTDAKGAPKDA
                     VIKHGSSFASANMSGTGPFIVTGREQGIKLTLKRFKDYWDTDSPGNVDEIVFTPIKEP
                     ATRVAALLAGDVDFIGPVPPTDLKRVKRVGCCTVVTMASPRILTFQLNQARVDAFKDP
                     RVRKAMSYAINREGIAKKIMRGQATPAGQLSPPGYAGHNEALTPPYDLAKAKALMKDA
                     GYEDGFSVTMIAPNNRYVGDSRIAEAVAAMLAKINIKVDLQTLPKAQYWQRFDARDGD
                     MLMIGWQSDTQDSANFYEFLVMTPDAETGYGQYNAGDYSNPQVDRLTLQTQTMTDEAA
                     RAEVLKSIERMLADDAALLPLQWQHLAWAARENVKIGPVVNVIDMPYLGDLVVE"
     gene            1221..1679
                     /locus_tag="AC16Y7_08225"
     CDS             1221..1679
                     /locus_tag="AC16Y7_08225"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5709405.1"
                     /translation="MFKKILLVLVALLVVFLVVVALQPSEYRVERSTTVAAAPSQVFG
                     HVNDFHKWEAWSPWAKIDPDAMITFEGPEAGQGAIMIWDGDDKVGAGKMTLVESIPDK
                     AVKTKVEFTRPYEGSTNSDFSFTPKGDQTEVSWAMHGTHNFIWRRRFASS"
     gene            1880..2506
                     /locus_tag="AC16Y7_08230"
     CDS             1880..2506
                     /locus_tag="AC16Y7_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709406.1"
                     /translation="MKWDFLNSNTVPRPPARGRAIAIMLSVGVASGLIASIPSPLPEI
                     RLEDEGLLLNAEGNPLHAGIVFGLAVASMMWLWVTHDIAKCLLAFAVVLLGWLAAVNT
                     ANDVFQAVVGSELFGTVQGAKSRREVIGLLAGGLSGAAVGAGLTAFGSGIPSERIRKP
                     ENWIRIVIVGTLVGVLLYPATELNALVLLFVPWQALVAAAIAVGLTRA"
     gene            2514..3506
                     /locus_tag="AC16Y7_08235"
     CDS             2514..3506
                     /locus_tag="AC16Y7_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5709407.1"
                     /translation="MSAFASAATFIARRLLQAALVMLAILVIAFAVRQSLGDPLREIM
                     GQSVPESERAELRHELGLDAPWPVQFARYLGRAATGDLGTSIIYKKPTVEVVLGKFPA
                     SFELVLGASLIVLLLSLPGGVYCAVRPQSLGARLVLGLSVLGISVPVFLTGIILITVF
                     SVWLGWLPAFGRGQTVDLGFWQTGLLTRDGLAHLLLPAVTLSSIMLPLFIRLVRGAML
                     DELGHDYVRTARAKGASPLRVWIVHALRNALLPLVAVGGLQAGTLIAYTLLTETVFQW
                     PGMGFLFLEAVTRADIPVITTYLVLVGLLFVVVNTLVDLVSLALDPRVSLEGAR"
     gene            3503..4423
                     /locus_tag="AC16Y7_08240"
     CDS             3503..4423
                     /locus_tag="AC16Y7_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5709408.1"
                     /translation="MSLSTAPAADRAFATFAAERPGAVLAGLLLAIVGVAALFAPLIA
                     PQDPFDLASFDILDAELPPIWQEGSDPRFVLGTDAQGRDLFSAILYGTRISLLVGILA
                     VAIQAAIGVSLGLAAGYSGGRVDSLVTRLADIQLSLSTLMMAIIVMALFRAGLGGEAL
                     SQFAVPLLVLVIGLAEWPIFARTARAATLVEVSKDYVRAARAVGSGDAAIVQRHILPN
                     ILSPLVVIATTQVAGAIMAEAALSFLGLGMPVTKPSLGTLIRSGYDLIFAGAWWVTVL
                     PGLVLIGVLIAVNVLGDALRDWLDPRARAG"
     gene            4566..5561
                     /gene="glpX"
                     /locus_tag="AC16Y7_08245"
     CDS             4566..5561
                     /gene="glpX"
                     /locus_tag="AC16Y7_08245"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HIR5709409.1"
                     /translation="METPGPVNPDLVYSLRRVTERAACAAFNWIGRGDADDGGRAALD
                     AMRAAIAEIDIDARVVIGEATAGEAPQPQRGEHLGSPGAAFKADIAVDPVEGTSYLVR
                     GLTNALSVLAVAPRGAMMNPGPAFYMEKFVASAPARGKIDPAWSTSKKLEELAGILNK
                     DISDLTVFVLEKPRHRALVNEILAAGARVAMYPAGDVAGALMAAVPGSSIDALMGTGG
                     TPEGVMSACAVRAIGGEFLARLDPQLQTETQAVKDAGIDTQRWFERDELITSDDVFFC
                     ATGITTGLMLEGVEKSKTHYKLQTMMITGASGERQIMTSYLPIERLASVAARDVA"
     gene            5597..>5889
                     /locus_tag="AC16Y7_08250"
     CDS             5597..>5889
                     /locus_tag="AC16Y7_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709410.1"
                     /translation="MPKRVDHPIILGIVGDSASGKTTLSAGVAQILGEEHCSIFCTDD
                     YHCYTRQERNDAGMSALDPRANHIDVLEQHLRLLRRGEPVLKPVYCHHDGTLGR"
BASE COUNT         1001 a         1870 c         1883 g         1135 t
ORIGIN      
        1 cgatcaacgc gcacactgtc gactttgtga ccaaaggccc gtacccgctg gtgctcaatc
       61 tcgccaccta catcttcccc atggaccgcg agttctattc gggcacggat gcgaagggtg
      121 cgccgaaaga cgccgtgatc aagcatggct cgtcctttgc ctcggcgaac atgagtggca
      181 cggggccgtt catcgtcacc ggccgcgagc aaggcatcaa gctgactttg aagcgcttca
      241 aggactattg ggacacggac tcgcccggaa acgtggacga gatcgtcttc acgccgatca
      301 aggaaccggc cacacgcgtc gcggcgcttc ttgccggcga tgtcgacttc atcggacccg
      361 taccgcccac agacttaaag cgcgtcaagc gggtcggctg ttgcacggtg gtgaccatgg
      421 cgagcccccg tatcctgacc ttccagttga accaagcccg cgtcgacgcc ttcaaggatc
      481 cgcgtgtgcg caaagccatg agctacgcca tcaaccgtga gggcatcgcc aagaagatca
      541 tgcgtgggca ggccaccccc gccggccagc tcagtccgcc gggttatgca gggcacaacg
      601 aggccctcac gccgccctac gacctcgcca aagccaaggc actgatgaag gacgcgggtt
      661 acgaggacgg cttctccgtc accatgatcg cgcccaacaa ccgctatgtc ggcgactcgc
      721 gcattgccga ggcggtcgcc gccatgctgg ccaagatcaa catcaaggtc gatctccaga
      781 cgctacccaa ggcgcaatac tggcagcgtt tcgatgcgcg cgacggcgac atgctgatga
      841 tcggctggca gtccgacacg caagactcgg caaacttcta cgagttcctg gtgatgacgc
      901 cggacgctga gacgggctac ggccaataca acgccggcga ctattcgaac ccgcaagtcg
      961 atcgcctgac cttgcagacc cagaccatga cggacgaggc ggcgcgcgct gaggttctca
     1021 agagtatcga acgcatgctt gccgacgacg ccgcgcttct gccgctgcaa tggcagcatc
     1081 tcgcatgggc ggcgcgcgaa aatgtgaaga tcgggcccgt ggtgaatgtc atcgacatgc
     1141 cgtatctcgg tgatctcgtc gtcgaatagg tcgacggccg aagaggccca tctaaaaagg
     1201 tcaatgggga gggaagtcgg atgttcaaga agattctgct cgtgcttgta gcgctgctgg
     1261 tcgtgttcct ggtggtcgtc gcgctccagc cgtccgagta ccgtgtcgag cgcagcacga
     1321 cggtcgccgc cgcgccgtcg caagtcttcg gccatgtgaa cgactttcat aaatgggagg
     1381 cctggtcgcc ctgggccaag atcgatcccg acgccatgat caccttcgaa ggtcccgagg
     1441 cgggccaagg cgcgatcatg atctgggacg gcgacgacaa ggtcggcgcc ggcaagatga
     1501 ccttggtcga aagcattccg gacaaggccg tcaagaccaa ggtggaattt acccggccct
     1561 acgaaggcag caccaactcg gatttcagct tcactccgaa gggcgatcag acggaggtct
     1621 cctgggccat gcacggcacg cacaacttca tatggagaag gcgttttgcc tcgtcatgaa
     1681 cggcaccggc atgatgggca aagacctcga caaggggctg gcccagctga agtccgtgtc
     1741 ggagtagggc taagcaggcc cggccccacc ccttcaatta gtgcactaga gccccgtcag
     1801 gtatagtgat cccggcgcgc atccgcgcgg ggatcggtta agggggcggc agcgtaagtg
     1861 gatttgcccg ctatcttgaa tgaagtggga tttcctgaac tccaatacgg tgccacggcc
     1921 gccggcgcgc ggccgggcca tcgccatcat gttgagcgtg ggcgtcgcat ccgggctcat
     1981 cgccagcatt ccctcgccat tgcccgaaat ccgtctcgag gacgaaggcc tgctcctgaa
     2041 cgcggaaggt aatccccttc acgctggcat cgtcttcggg ctcgccgtcg cctctatgat
     2101 gtggctatgg gtgacccacg acatcgccaa atgcctgctc gccttcgcgg tcgtcttgct
     2161 tggctggctt gccgcggtca acacggcgaa cgacgtgttt caagccgtgg tcggctcgga
     2221 gctgttcggc accgtgcagg gtgcgaagtc gcgccgcgag gtcataggac ttctggcagg
     2281 cgggttaagc ggggcggcgg ttggcgcagg tctgacggcg ttcggatccg gaatcccttc
     2341 ggagcgcatc aggaagcccg agaactggat tcgcatcgtg attgtcggaa cgcttgtggg
     2401 cgtgctgctt tacccggcca ccgagctcaa tgcactcgtt ctgctcttcg tgccgtggca
     2461 ggcgcttgtc gcggcagcta tcgcggtcgg gctgactcgg gcgtgagtcc gtcatgagcg
     2521 cgttcgcttc tgccgccacc ttcatcgcgc gcaggctcct gcaagcggcg ctcgtcatgc
     2581 tggcgattct ggtcatcgcg tttgccgtcc ggcaaagtct tggcgatccc ttgcgcgaga
     2641 tcatgggtca gtcggttccg gagtccgaac gtgcggagct gcgccatgaa cttggcctgg
     2701 acgccccatg gcccgtgcag ttcgcgcgct atttgggacg cgccgcgacg ggcgatctcg
     2761 gcacgtccat catctacaag aagccgactg tcgaggtcgt gctcggtaaa ttcccggcga
     2821 gcttcgagct tgtgttgggc gcgagcctca tcgtgctttt gttgtcactg cccggcggtg
     2881 tctattgcgc ggtccgtccg caaagtctcg gcgcgcgctt ggtcttaggg ctcagtgtgc
     2941 tcggcatctc ggtgcccgtg tttctcaccg gcattatcct catcaccgtc ttttccgtgt
     3001 ggctcggctg gctgccggcc ttcgggcgcg ggcaaacggt cgatctcggg ttttggcaaa
     3061 cgggactcct gacgcgtgac gggctcgcgc acctcttgct tccggcggtg acgctgtcct
     3121 cgatcatgct gccactgttc attcggctgg tgcgcggtgc catgctcgat gagcttggcc
     3181 acgactacgt gcgcaccgcc cgcgccaagg gcgcatcgcc gttgcgcgta tggatcgtgc
     3241 atgcgctgcg caacgcgctt ctgccgctgg tggccgtggg cgggctccaa gccggcacgc
     3301 tcatcgctta cacacttctg acggagacag tgttccagtg gcccggcatg ggctttctgt
     3361 ttctggaagc ggtgacacgc gccgacattc cggtgatcac cacctatctc gtgctggttg
     3421 gcttgctgtt cgtcgtcgtc aacacgctcg tggaccttgt aagcctcgcg ctcgatccgc
     3481 gtgtgagttt ggagggcgcg cgatgagcct cagcacagcg ccagccgcgg accgcgcgtt
     3541 cgccaccttc gccgccgagc ggcccggcgc ggtgctggcc ggacttcttc ttgcgattgt
     3601 gggcgtcgcc gcgctgttcg caccgctgat tgcgccgcaa gatccgttcg acctggcgag
     3661 cttcgatatt cttgacgccg agctgccgcc catctggcag gaaggcagcg atccgcgctt
     3721 cgtactcggc accgacgcgc aaggccgcga tctgttcagc gcgattttgt acggcacccg
     3781 catctcgctg ctagtcggca ttcttgctgt ggcgatccaa gccgcgatcg gcgtctcgct
     3841 ggggctcgca gcaggctatt ccggggggag ggtggacagc ctcgtcacgc gcctggccga
     3901 tatacagctc tcgctctcca ccctgatgat ggcgatcatc gtcatggcgc tgttccgggc
     3961 cgggctgggc ggcgaggcct tgagccaatt cgccgtgccg ctgctcgtcc tggtcattgg
     4021 cctcgccgag tggccgattt tcgcccgcac cgcccgcgcg gcgaccctcg tggaggtgtc
     4081 caaggactac gttcgcgcgg cccgggccgt gggaagcggc gatgctgcca ttgtgcagcg
     4141 ccacatcctg cccaacatcc tctcgccgct ggtcgtcatc gccacgaccc aggtggccgg
     4201 cgccatcatg gcggaggcgg ctctctcatt cctaggcctg ggcatgccgg taaccaaacc
     4261 ctcgctcggc acgttgatcc gctccggcta tgacctcatt ttcgccggcg cctggtgggt
     4321 tacggtcctc ccagggctgg tgctcatcgg ggtcttgatc gcggtcaatg tgctgggaga
     4381 cgccttgcgc gattggctcg acccgagggc tcgggccggc taaatcaagc ccgcggaagc
     4441 ggctattcgg cccaggcccc cttgatcatt ggggccccag cgacccataa gatatagtgc
     4501 agcaaggctt tccggccacg agaacagcgg cgtttcggat acgaagccgc cgcggaggag
     4561 acgtcatgga gaccccgggc ccggtcaatc cggatctcgt ctattctttg cgccgtgtca
     4621 ccgagcgcgc ggcctgtgcg gccttcaact ggatcggccg cggcgatgcc gacgacggtg
     4681 gccgggctgc cctggacgcc atgcgtgccg cgattgccga gatcgatatc gacgcgcgcg
     4741 tcgtcatcgg ggaggccacg gccggcgagg cgccgcagcc gcaacgcggc gagcatctcg
     4801 gctcccccgg cgccgctttc aaagcagata tcgccgtgga cccggtggaa ggcacgagct
     4861 atctggtgcg cggcctcacc aacgcccttt ccgttctcgc cgtggcgccg cgcggcgcca
     4921 tgatgaaccc aggccccgcc ttctacatgg agaagttcgt ggccagcgcg cccgcgcgcg
     4981 gcaagatcga cccggcctgg tccacctcga aaaagctcga ggagctggcc ggcattctca
     5041 ataaggacat ttccgatctc accgtgttcg ttttggagaa gccgcgtcac cgcgctctcg
     5101 tgaacgagat tctcgcggcg ggagcgcgcg tcgccatgta tccggcgggc gacgtcgccg
     5161 gcgcactcat ggcggcggtt ccggggtcga gcatcgatgc tttgatgggg acgggcggca
     5221 cgccggaagg cgtgatgtcg gcctgtgccg ttagggcgat cggcggcgag ttccttgcgc
     5281 gcctcgaccc gcagcttcag acggaaacgc aagcggtcaa ggacgccggc atcgacacgc
     5341 agcgctggtt cgagcgcgac gaactcatca cctccgacga cgttttcttt tgtgcgacgg
     5401 gcatcaccac ggggctcatg ctcgaaggcg tagagaaatc gaagacccac tataaattgc
     5461 agacaatgat gattaccggg gcgtccggcg aacggcagat catgacgagt tatctgccga
     5521 ttgaacgtct tgcgagcgtg gcggctcgcg acgttgcgta acaagccaaa cggataggaa
     5581 cgcctgggag cagagcatgc ccaagcgagt ggatcatccg atcatcctcg gcatcgtggg
     5641 tgactcggca tcgggaaaga cgaccctgtc ggcgggcgtc gctcagattc tgggcgagga
     5701 gcactgctcg atcttctgta ccgatgacta tcattgttac acgcggcaag agcgtaacga
     5761 tgcggggatg tccgcgctcg atccacgcgc gaatcatatc gacgtgctgg agcagcacct
     5821 gcgtctcttg cggcgtggcg agcccgtgct caagccggtc tattgtcatc atgatggcac
     5881 gctggggcg
//
LOCUS       DBNHVU010000194         4387 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_1854607, whole genome shotgun sequence.
ACCESSION   DBNHVU010000194 DBNHVU010000000
VERSION     DBNHVU010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_1854607"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="AC16Y7_08255"
     CDS             <1..401
                     /locus_tag="AC16Y7_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PdxA family dehydrogenase"
                     /protein_id="HIR5709411.1"
                     /translation="LAVSGLNPHAGEDGSLGREELEIIAPAIEAARNAGLDVSGPYPA
                     DTLFHMPARERYDAAICMYHDQALVPFKTLAFDEGVNVTLGLPFVRTSPDHGTAFDIA
                     GTGEANPRSLIEALRLAEAMSESAADETVP"
     gene            398..1243
                     /gene="rsmA"
                     /locus_tag="AC16Y7_08260"
     CDS             398..1243
                     /gene="rsmA"
                     /locus_tag="AC16Y7_08260"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR5709412.1"
                     /translation="MTTPDGLPPLREVIRSAGLTAKRSLGQNFLLDLNLTGRIARAAG
                     PLEGVTVVEIGPGPGGLTRALLMEGAGAILAIERDDRCLAALDQVATAYPGRLDVLAA
                     DALDVDYGALQLPRPARIVANLPYSVGTPLLIGWLKTEPWPPWFDRLVLMFQREVAER
                     IVATPRTKAYGRLSVLSQWRAQTRILFNVPPDAFTPKPKVSSAIVEFVPKPAPTPPCD
                     AAQLETVTAAAFGQRRKMLRASLRQIVQDPETLLTGLGLDPKARAEELEVADFCRIAN
                     ALKDR"
     gene            complement(1240..1872)
                     /gene="gmk"
                     /locus_tag="AC16Y7_08265"
     CDS             complement(1240..1872)
                     /gene="gmk"
                     /locus_tag="AC16Y7_08265"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR5709413.1"
                     /translation="METIGRRGLMLVLSSPSGAGKTTIARRVLDEDEGVAASISHTTR
                     EQRPGEVDGQDYHFVDKKAFSRMREQGDFLEWAVVFDNYYGTTRDPVEQALGSGQDVL
                     FDVDWQGADALRKSARNDVVSVFVLPPTADALEQRLKTRAQDSAAVVVRRMRGASNEI
                     QHWKDYDYVLVNFDVDQSVAAVHAILAAERLRRTRLTGLNEFVEGLLSQL"
     gene            complement(1881..2768)
                     /locus_tag="AC16Y7_08270"
     CDS             complement(1881..2768)
                     /locus_tag="AC16Y7_08270"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016553440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIR5709414.1"
                     /translation="MTLKSMTGFARTDGLHGETSWFWEVRSVNGRSLDLRLRLPSGLE
                     RLEAGARGLCQERLARGNCTVNLGVKRDAGQTEIRLNETALGQALAVAKQASTLSGLE
                     PAQLDTLLGMRGVVETAEPEDSEEKQAALGHALLAGLAAALDQLVETRTAEGERLQQA
                     IETQISSIDTLVAQAADAVARQPAILAERLREQIGRLAEAGATLEPERLHQEALLLAA
                     KADIQEEIDRLRAHVEAANELIASGAPVGRKLEFLAQEFNREANTICSKASDVEISRT
                     GLELKSVIDQLREQVQNIE"
     gene            complement(2873..4117)
                     /gene="mltG"
                     /locus_tag="AC16Y7_08275"
     CDS             complement(2873..4117)
                     /gene="mltG"
                     /locus_tag="AC16Y7_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR5709415.1"
                     /translation="MPGPAGQSLPRSPAEALEPQRPPELRAQEEVEENDRPFLRMLDG
                     LVNAVFILACMAIGAFYFIHSEFNRPGPLQVSTVFVVPKGEGTAAIAERLTAEGIITD
                     RRIFMASIFYFMHLKGQGSLKAGEYQFDKHASMRQVLDTLVEGKSIQYRVTFPEGWTS
                     QQIVQRLAANTQLNGPVPAVPAEGSLLPDTYSFGTKDTREDILQRMAVAHQKYLAKLW
                     EERDPDIMVKTPEEAVILASMVEKETGVAEERPHIASVFHNRLRKRMRLQSDPTIIYG
                     IFGGSGMRDHPITREELDRPNPYNTYKIDGLPPTPIGNPGRAAIEAVLKPAKTKDLYF
                     VANGKGGHFFSATLAQHNSNVVKWRKIEREIRARQKAEAEAAAAAEAAKDHGVLLPEG
                     EVGLTVVDGPPAIPLRNPSRTP"
BASE COUNT          778 a         1481 c         1328 g          800 t
ORIGIN      
        1 ggctcgccgt gagcgggctc aatcctcatg cgggcgaaga cggcagcttg ggacgcgaag
       61 agcttgagat catcgcgccc gccattgagg cggcgcgaaa cgcagggctc gacgtctcag
      121 gcccctaccc ggccgacacg ctgttccata tgccggcgcg cgagcgttac gacgccgcca
      181 tctgcatgta tcacgaccag gcgctggtgc cgttcaagac actcgccttc gatgaggggg
      241 tgaacgtcac gcttggactc cccttcgtgc gcacctcgcc cgaccatggc acggcttttg
      301 acatcgccgg aacgggagaa gcaaatccgc gaagcctcat cgaagcgctt cgtctcgccg
      361 aagccatgag tgagagcgcc gccgacgaga cggtgccgtg accacgccag acggcttgcc
      421 gcccttgcgc gaggtcatcc gcagtgccgg acttacggca aagcgaagcc tcggccagaa
      481 cttcctgctc gacctcaatc tgacgggccg catcgcgcgc gccgccgggc ctctcgaagg
      541 cgtaacggtc gtcgagatcg gccccggccc gggcggcctc acccgtgcac tcctcatgga
      601 aggcgcgggc gccatcctcg ccatcgagcg cgacgatcgt tgccttgccg ccctagacca
      661 ggtcgcgacc gcctatcctg gccgtctcga cgtccttgcc gccgatgcgc tggacgtcga
      721 ctatggggcg ctgcagctcc cacggccggc ccggattgtg gccaacctgc cctatagcgt
      781 cggcacgccg ctcctcatcg gatggttgaa gaccgagcct tggccgcctt ggttcgacag
      841 gctcgtactc atgttccagc gcgaagtggc cgagcgcatc gtcgccacgc cacgcaccaa
      901 ggcctatggc cgcctgtccg tgctgtccca atggcgcgcg cagacgcgca tactctttaa
      961 cgtgccgccg gacgctttca cgccaaagcc gaaggtctcg tccgccatcg tggaattcgt
     1021 gccgaagccg gccccgacac caccttgcga tgccgcacag ttggagacgg tcaccgcggc
     1081 cgcgttcggt caaagacgca agatgctgcg cgccagcctc cgccagatcg tgcaagatcc
     1141 agagactctg ctcacgggtc ttggccttga tcctaaagcg cgcgccgagg agttggaggt
     1201 tgctgacttc tgccggatcg cgaacgcgct caaggatcgt tagagctgcg acagaaggcc
     1261 ctccacgaat tcgttcaacc cagtgagacg ggtgcgtctt agacgctccg ccgccagaat
     1321 ggcatgcacc gcggcaaccg attgatcgac gtcgaagttg acgaggacat aatcatagtc
     1381 cttccagtgc tggatctcgt tgctggcgcc gcgcatgcgc cggacgacga cggcggcgga
     1441 gtcttgtgca cgcgtcttga gccgctgctc aagggcgtcg gccgtgggcg gcagaacgaa
     1501 gacggaaacc acatcgttcc ttgcggactt gcgcaaggca tcagcgccct gccaatccac
     1561 gtcaaacaac acgtcctggc ccgaacctaa ggcctgctcc acggggtcgc gcgtcgtgcc
     1621 gtagtagttg tcgaacacga cggcccactc cagaaaatcc ccctgctccc gcatccggct
     1681 gaaagccttc ttgtctacga agtggtagtc ctggccatcc acttcgccgg ggcgctgttc
     1741 gcgtgtcgta tgggaaatcg aggcggcaac cccctcgtcc tcgtcaagca cgcgccgcgc
     1801 gatcgtcgtc ttgcccgcgc ccgacggcga cgacagcacc agcatgagtc ctcgacggcc
     1861 gattgtctcc atgggaggcc tcactcgatg ttttggactt gctcgcgaag ctgatcgatc
     1921 accgatttga gctccagccc cgtccggctg atctcaacat cggaggcctt ggagcagatc
     1981 gtattggctt cgcgattgaa ctcttgcgcg aggaactcca gcttccgccc gaccggtgcg
     2041 ccgctggcga tcagttcgtt ggcggcctca acatgggcac gcaagcgatc gatctcttct
     2101 tggatgtcgg ctttcgcagc cagcaacagc gcttcttgat gcaaccgctc gggctctagc
     2161 gtggcgccgg cctcggcaag ccgcccaatc tgttcgcgaa ggcgctccgc gagaatcgcc
     2221 ggctgacgcg cgacggcgtc ggccgcttgg gcaaccaacg tgtcgatgct ggagatctgc
     2281 gtttcgatgg cctgctgcag gcgttcgcct tcggcggtcc gcgtctccac cagctgatcc
     2341 agagccgccg caagcccggc aagcagcgca tgtccaaggg ccgcctgctt ctcctcgctg
     2401 tcctcaggct ccgcggtctc caccacaccc cgcatcccaa gcagcgtatc gagctgggcc
     2461 ggttcgagac cactcaaagt gctcgcctgt ttggcgacag ccagcgcctg gccaagcgcg
     2521 gtctcattga gccggatctc cgtctgcccg gcgtcacgct tcacaccgag attgaccgtg
     2581 caattgccgc gcgccaaccg ctcctggcaa agaccgcgcg cacccgcctc aagccgttcg
     2641 agacccgacg gaagccgtaa ccgcaggtcg agcgaccgcc cgttgacgct gcgcacttcc
     2701 caaaaccagc tggtctcccc atgcagcccg tcggtgcggg caaagccggt catgctcttc
     2761 aaggtcatcg acattgcctc gctcaactga ggggctcgcc cggctttata gccgagtcga
     2821 gaacaaaatc aggcctgaag gtacccaaag ctgcaaaatg gggtgaagag actcaaggcg
     2881 tgcggctggg attgcgcaac gggatggccg gcggaccgtc cacgacagtg agcccgacct
     2941 cgccttcagg cagcaggacg ccatggtcct tggccgcctc cgctgccgcc gccgcctcgg
     3001 cctcggcttt ctgccgcgcc cggatctcac gctcgatctt gcgccacttg accacgttgc
     3061 tgttgtgctg ggccagggtc gcagagaaga agtgtccccc cttcccgttg gcaacgaaat
     3121 acagatcctt ggtcttcgcg ggcttcagca ccgcctcgat cgccgcacgt ccaggattgc
     3181 cgatcggcgt cggcggcaag ccatcgatct tataggtatt ataggggttt ggacgatcaa
     3241 gctcttcccg cgtgatcgga tggtcacgca tgccagaccc gccaaagatg ccatagataa
     3301 tggtggggtc ggactgaagg cgcatccgct tgcgaagccg gttgtgaaag acgctggcaa
     3361 tgtgcggccg ctcctcggca acgccggttt ccttctcaac catggacgcc aaaatcaccg
     3421 cttcctccgg cgtcttgacc atgatgtccg ggtcacgctc ctcccacagc ttcgccaggt
     3481 atttttgatg cgcaacggcc atgcgttgga gaatgtcctc ccgcgtgtcc ttggtgccga
     3541 aactatatgt atcgggcaga agcgagccct cggcaggtac tgccggcaca ggtccattca
     3601 gttgcgtgtt ggccgccagc cgctgaacga tctgctggct cgtccagcct tccgggaacg
     3661 tgacccggta ctggatagat ttgccttcga ccaaggtgtc caggacctga cgcatggagg
     3721 cgtgcttatc gaactgatac tcgccggcct ttagcgaccc ctgccccttc aaatgcataa
     3781 agtagaagat gctcgccatg aagatgcggc ggtcggtgat gataccttcc gctgtgagcc
     3841 gttccgcgat cgccgccgta ccttcgccct tcggcacgac aaacacggtc gagacctgga
     3901 gcgggcccgg acgattgaac tccgaatgta tgaagtagaa ggcgccgatg gccatgcagg
     3961 cgagaataaa gacggcgttg acgagaccat cgagcatccg caggaacgga cgatcgttct
     4021 cctcaacctc ttcttgagcg cgaagctcgg gtggacgttg tggctccagc gcctcggctg
     4081 gactgcgcgg cagtgactgc cccgccggtc cgggcatggg gatcgggcgc cccgttccat
     4141 aaccaccagc cccttgacct ggcgtcccag gacccggcgc cgcttgcccc ggcgttccag
     4201 gacgcgcggc cccatgacct tgcgtcccat gacctggcga tccaggacgc gccgttcccg
     4261 gacgcagcgg gggacgcccg ggcggattcc ccggtggttt cccgggagga gacggtggtg
     4321 gtgggggagt attcctcgaa gaagtcatgc tggaccagcg cgcgcccccg cggatgtcta
     4381 agccact
//
LOCUS       DBNHVU010000195         9265 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_145054, whole genome shotgun sequence.
ACCESSION   DBNHVU010000195 DBNHVU010000000
VERSION     DBNHVU010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 9265)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9265)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9265
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_145054"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="AC16Y7_08280"
     CDS             <1..956
                     /locus_tag="AC16Y7_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR5709416.1"
                     /translation="QRRPEPVPRDDEAPVTGLLDSLRKEYVQSAGDKPKRTQPARRKS
                     ALFDRLRQARPKKKETAPLSNPPSQGNDMASLSPNDLRHYLTQRITTTEPDLLDAMPR
                     TRKAGGQAAGQVLQSLDTVLDKVLERATGGLPRALLVAGTSPKADATDAAIHLARALV
                     DRNEQVVLVDLTKGGSAVSGPLGMPRVPGFTDLAAGRAQFADVIRVDDDSPLQVITAG
                     NPTTRGPDPEPDSFMRVFEALTQAYGCVVLHADLIAIEALKPALKFELPAMVAVLPAR
                     GTVADEGDALSTFQSLGCPVFIYDGKGKSRRSGLLGRIAAV"
     gene            complement(965..1291)
                     /locus_tag="AC16Y7_08285"
     CDS             complement(965..1291)
                     /locus_tag="AC16Y7_08285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5709417.1"
                     /translation="MEIALSSLAEGETQRQSPGALLLRHQIRDTCKAGLAYFDLGVGK
                     ARHKDEWCNTDQALFDSFIALKPQGLFVTLPRATVARLKRTVKSNRYLWSLAQTVRKR
                     LRGNGA"
     gene            complement(1327..1530)
                     /locus_tag="AC16Y7_08290"
     CDS             complement(1327..1530)
                     /locus_tag="AC16Y7_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2842 domain-containing protein"
                     /protein_id="HIR5709418.1"
                     /translation="MSAHLRKLIGTVVLLVFLVLYIWLAAALGSGRIVEAPALLQAAY
                     FLVAGLLWVIPAGLLIRWMQRPD"
     gene            1570..2682
                     /locus_tag="AC16Y7_08295"
     CDS             1570..2682
                     /locus_tag="AC16Y7_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR5709419.1"
                     /translation="MSWAAEPAITAFMSPKATETSTAPMPAGNVRALRLWLGCIAFLI
                     VAMIFVGGATRLTDSGLSITEWQPIVGAIPPLSDGDWQEAFEAYKQIPEYTQVNKGMS
                     LSAFKRIYWWEWAHRFLGRFIGLAFFVPFVCFWAAGYIPRPLMPKLIGLFMLGGLQGA
                     LGWYMVKSGLVDRVDVSQYRLAAHFGLAVAILGYTLWLLLGLSERQAARGGAPAIVAW
                     LVLLLIFVQLLSGALVAGIDGGLGFNTWPLLNGAFIPSGLGEASPWYLNLFENPLTVQ
                     FDHRMLGYAVVVATVLQAIWLAVRGHQTLVGSAATLAVLSVLQATLGVWTLLLAVPLE
                     LGLAHQAGAILVFAAALYHAWLAGHEPAEAQTAASA"
     gene            complement(2661..3947)
                     /locus_tag="AC16Y7_08300"
     CDS             complement(2661..3947)
                     /locus_tag="AC16Y7_08300"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018697734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HIR5709420.1"
                     /translation="MSEERTPGFNTLAIHAGAQPDPTTGARVTPIYQTTSFVFDDVDH
                     AAALFNLEQFGNIYTRIMNPTQAALEERMAALEGGTAALAVASGHAAQAIVFHTLMEP
                     GDEFIAARQLYGGSINQFGEAFKKFDWHVKWADTTDAESFRAQVTPKTRAIFVESIAN
                     PGGVITDLEAISAVAKEAGVPLIVDNTLASPYLTRAKDHGADIIVHSLTKFLGGHGNS
                     IGGVIVDCGTFDWMGSGRYPTITAPCPSYHGMTLGETFGNFAFAIAARVLGLRDLGPA
                     IAPMNAFLVLTGIETLPLRMQRHCDNALKVSTWLSGHDKVAWVNYAGLPDNAFHALAQ
                     RYCPAGAGAVFTFGLKGGYDAGIALVSKVELFSHLANIGDTRSLIIHPASTTHRQLSD
                     ENRESAGAGADVVRLSVGLESVDDIIADLDQALAAV"
     gene            complement(4029..4823)
                     /locus_tag="AC16Y7_08305"
     CDS             complement(4029..4823)
                     /locus_tag="AC16Y7_08305"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HIR5709421.1"
                     /translation="MVEHDILLQEAQGPVLVLTLNRPGARNCLSMDLMTALHRAIDGA
                     GAADAVRAVVIAGHGPAFSSGHDLKELTAHRDDPDKGGSFYADTMRVCSDMMLAIVRC
                     PKPVIAAVNGIATAAGCQLVATCDLAVAADDARFATPGVNIGLFCSTPMVALSRNVSR
                     KAAMEMLLFGDMVEAEDAKTLGLINRVVPADQVVNEAIAFGRKIAEKPARTVKTGKEA
                     FYTQAEMTLEEAYRYTADVMVANMLGAESQEGIGAFLDKRTPNWPR"
     gene            5031..5495
                     /gene="rplM"
                     /locus_tag="AC16Y7_08310"
     CDS             5031..5495
                     /gene="rplM"
                     /locus_tag="AC16Y7_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004441288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR5709422.1"
                     /translation="MKTFSATPKDIEKSWVLIDASGLVVGRLAALIATRLRGKHKPGY
                     TPHMDDGDNIIVINASDVVFTGNKRANKEYFWHTGYPGGIKSRTAEKVLESKNPERVL
                     QKAVQRMLPDGPLARKQLKNLKVYAGSEHPHEAQNPEKLDIAALNSKNARSA"
     gene            5519..5989
                     /gene="rpsI"
                     /locus_tag="AC16Y7_08315"
     CDS             5519..5989
                     /gene="rpsI"
                     /locus_tag="AC16Y7_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007692725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR5709423.1"
                     /translation="MEDLAQLKGEGEGDAPAPERVYEKKVDAQGRAYATGKRKDAVAR
                     VWVKQGPGNIIVNGKDHTAYFARPVLQMILKQPLVTADREGQIDIVCTVSGGGLSGQA
                     GAVRHGISKALTHFEPELRSVLKKAGFLTRDARTVERKKYGKAKARRSFQFSKR"
     gene            6100..7149
                     /gene="argC"
                     /locus_tag="AC16Y7_08320"
     CDS             6100..7149
                     /gene="argC"
                     /locus_tag="AC16Y7_08320"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR5709424.1"
                     /translation="MTSNQPIRAAVLGASGYTGADLIRLAARHPNIQITALIAKAHAG
                     QPLGQVFPHLGSLDLPQLVTSDEVDWDSIDVAFCGLPHGTAHVEIAKLPERVKVIDMS
                     ADFRLRDLSTYAEWYGGEHGAPQLLSDAVYGLTEHYRDEIAGARIVACPGCYPTAVLL
                     ALLPLVKEKLIASEGIIIDAKSGVTGAGRSLKQNILFSEAGEGLSPYGIGSHRHVPEI
                     EQEIGMAANQAVIVNFTPHLAPMARGELCTCYVQLPSGTTDADLRGALRDAYAASPFV
                     RVMDEGVIPATQHVRGSNYCHVGVFADRIPGRAIVVSAIDNLVKGSAGQALQNFNVMH
                     GLDESTALEQLPLFP"
     gene            complement(7157..8980)
                     /locus_tag="AC16Y7_08325"
     CDS             complement(7157..8980)
                     /locus_tag="AC16Y7_08325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHA/PHB synthase family protein"
                     /protein_id="HIR5709425.1"
                     /translation="MQERMDGADKPEADDSQTTQMDEFARNMVRLFDQGTKVFSTLAE
                     RSSTNGNNGGPYSMASEVTEAAKTLGEVAGAWVTNPNKLAAAQGDLFQNYADLWGRSV
                     RRFFGEEVEPVAEPEPGDNRFKDPEWSESQFFDFWKQAYLITSRWAEDVTKNTEGVDD
                     KTRKKALFYLEQMLAALSPSNNPFTNPEVVRATLQSNAENLVQGMTHFAEDLEQSKDL
                     LRISQTDLSAFEVGRDIAISPGKVVFQNDLIQLIQYSPTTEQVYERPLIMAPPWINKY
                     YILDLKPEKSLVKYAVDQGFTVFMISWVNPKAALARKTFEDYMTEGILAAVDAVDRQL
                     GLHHVNVLGYCVGGTLLASTLAYAAAKGDDRIATATFLTAQVDFSEAGDLLVFIDDAQ
                     LKSLEEMMAEQGYLDGSRMAAVFNMLRPRDLIWPYIVNNYLLGKKPFPFDLLYWNADS
                     TRLPAANHAFYLREFYHQNRLSKGEMELGGVKLDLSKVKIPIYELFTREDHIAPATSV
                     YRGSKLFGGPVRYVMAGSGHIAGVVNPPAKKKYQYWTGTPADTFDAWTKTAAEAPGSW
                     WPDWAEWLSGQSGEKIPARDPSAGALKPIEDAPGSFVKDRD"
BASE COUNT         1736 a         2908 c         2847 g         1774 t
ORIGIN      
        1 cacaacgacg gccggaaccg gtgccgcgcg acgatgaggc gcctgtgaca gggctgctcg
       61 acagcttgcg gaaggagtac gtccaaagtg ccggcgataa gcctaaacgc acgcaaccag
      121 cccgccgcaa gagcgcattg ttcgatcgcc tccggcaggc aagacccaag aagaaggaga
      181 cggcgccttt gtccaacccg ccgtcgcaag gtaacgacat ggcctcactc agccccaacg
      241 atcttcgtca ctatctgacg caacgcatca ccaccaccga accggacctt ctcgacgcca
      301 tgccgcgtac gcgcaaagcg ggcgggcagg ctgctggtca agttctccaa tcactcgaca
      361 ctgtgttgga caaggttctt gaacgggcga cgggtgggct gccgcgcgca cttctcgtgg
      421 ccggcacgtc gccgaaggcc gatgcaaccg atgcggccat ccaccttgcc cgagcccttg
      481 tcgaccgcaa cgaacaggtg gtgctcgtcg atttgaccaa gggaggctcc gccgtatccg
      541 gtccactcgg catgccgcgt gttccgggtt tcacagatct cgccgcgggc cgggcacagt
      601 ttgccgatgt catccgggtc gacgacgaca gccccttgca ggtcatcaca gccggcaatc
      661 ccaccacgcg cggaccggac ccggagccgg actccttcat gcgtgtgttt gaggcgctga
      721 ctcaggccta tggctgcgtg gtgttgcacg cagacctcat cgccattgag gccttgaagc
      781 ccgccttgaa gttcgagttg ccggcgatgg tcgccgtttt acctgcgcgc ggcaccgtgg
      841 ctgacgaggg cgatgcactg tcgaccttcc agtcgctcgg atgcccggtt tttatctatg
      901 atgggaaggg caagtctcgc aggtcgggac tgctcggccg gatcgcagcg gtctagcggc
      961 gtccttaggc cccgttgccg cgtaggcgtt tacgcacagt ttgcgccaaa gaccagagat
     1021 atcggtttga tttcacggtc cgcttaagcc gcgccacggt agcgcggggc agcgtcacaa
     1081 agaggccctg cggttttaac gcgatgaagc tgtcgaacag cgcctgatcg gtgttgcacc
     1141 actcgtcctt atgccgcgcc ttgcccacgc caaggtcaaa ataggcaagc ccggctttac
     1201 aagtgtccct aatctggtga cgcagaagga gcgcgccagg cgattgtctt tgtgtctcac
     1261 cctcggccag tgaggacaac gcgatctcca tacggtcttg cttgacgaaa gtcagtcggg
     1321 cccaaatcag tcaggacgtt gcatccagcg gatgagaagg cctgctggga tcacccacaa
     1381 cagccctgcc acaagaaagt aggcggcttg tagaagcgcc ggggcctcta cgatgcggcc
     1441 ggagccgagg gcggccgcga gccaaatata cagcaccaaa aagacgagca gcaccacggt
     1501 gccaatgagc ttgcggagat gcgcgctcac ggcaacctcc gctgcggcat tggggcaggg
     1561 gcggcacgat tgagttgggc ggcggagccc gctatcaccg cgttcatgag ccccaaggcc
     1621 acagagactt cgacggcacc catgcccgcc gggaacgtcc gggcgttgcg cctctggctt
     1681 ggctgcattg cgtttctcat cgtcgccatg atcttcgtcg gcggagccac acggctgacc
     1741 gattccggcc tgtcgatcac ggaatggcag cccattgtcg gcgccatacc gccgttatcg
     1801 gacggcgatt ggcaagaggc tttcgaggcc tacaagcaga tcccggaata tacgcaagtc
     1861 aacaagggca tgagcctttc cgcgttcaag cgcatctact ggtgggaatg ggcgcatcgt
     1921 ttcctcggcc ggttcatcgg cctcgcgttt ttcgtgccgt tcgtttgctt ttgggcggcg
     1981 ggctacattc cgcgcccctt gatgccgaag ctcatcgggc tcttcatgct cggcggtctt
     2041 caaggcgcac ttggctggta catggtcaag agcgggctgg tagaccgcgt cgatgtcagc
     2101 cagtaccggc tcgccgcgca tttcggactg gccgttgcga ttctaggcta cacactttgg
     2161 ctgctgctgg gcttgagcga acggcaagcg gcgcggggcg gcgctccggc cattgtcgcc
     2221 tggctcgtgc tgctgctgat ctttgtgcag ctcctctccg gcgcgctggt tgccggcatt
     2281 gacggcgggc tcggcttcaa cacctggccg ctcctcaacg gcgcgttcat tccgagcggc
     2341 ttaggcgagg cttcgccctg gtatctcaac ctgtttgaga acccgctgac ggtgcagttc
     2401 gatcaccgca tgctgggcta tgccgtcgtc gttgcgactg ttctgcaagc gatatggctc
     2461 gccgtgcgtg ggcatcaaac ccttgtcggc agcgccgcca cgctcgccgt gctgtctgtg
     2521 ctgcaagcaa cgctcggcgt atggacgcta ctgctcgcgg tgcctttgga actcggcctc
     2581 gcgcaccagg cgggcgcaat tctggtcttc gctgccgcgc tctaccatgc ctggctcgcg
     2641 ggccacgaac ccgcggaggc tcagaccgct gccagtgcct gatcgagatc ggcgatgatg
     2701 tcgtccacgc tctcaagccc gaccgataga cgcacgacat cggcacccgc gccggcgctc
     2761 tcgcggttct cgtcggacaa ttgccgatgc gtggtcgagg cggggtgaat gatcaggctg
     2821 cgcgtgtcgc cgatattggc gaggtggctg aagagttcga ccttgctgac gagtgcgatg
     2881 ccagcgtcat aaccgccctt caagccaaag gtgaacaccg cgccggcacc ggccgggcaa
     2941 tagcgctgcg cgagcgcatg gaaggcgttg tccggcaggc ccgcatagtt cacccaagcc
     3001 actttgtcgt gacccgacag ccatgtggag actttgagtg cgttgtcgca atggcgctgc
     3061 atgcgtaaag gcagcgtttc gataccggtc agaacgagga acgcgttcat cggtgcgatc
     3121 gcaggaccaa ggtcgcgcaa acccaggacg cgtgcggcga tggcgaaggc gaaattgccg
     3181 aaggtctcgc cgagtgtcat gccatgatag ctggggcagg gagccgtaat ggtcgggtag
     3241 cgcccgctac ccatccaatc gaacgtgccg caatcaacaa tcaccccgcc gatcgagttg
     3301 ccgtgaccgc cgagaaactt ggtcagcgaa tgcacgatga tgtcggcgcc gtgatccttc
     3361 gcgcgcgtga ggtaaggcga cgccagcgtg ttgtcgacga tcagcggcac acccgcctcc
     3421 ttggcgaccg cggagattgc ctccaggtcg gtgatgacgc cgccgggatt ggcgatagac
     3481 tcaacgaaga tggcccgggt tttgggcgtg acctgcgccc ggaaagactc cgcgtccgtg
     3541 gtgtcggccc atttcacgtg ccagtcgaat ttcttgaagg cctcgccgaa ctggttgatc
     3601 gacccgccat agagctggcg cgcggcgatg aactcatccc cgggctccat cagcgtatga
     3661 aacacgatcg cttgcgctgc atgccctgac gcgaccgcaa gggccgccgt accaccttcc
     3721 agcgctgcca tgcgctcttc gagcgcggcc tgggtcgggt tcatgatgcg ggtatagatg
     3781 ttgccgaact gctcgaggtt gaacagtgcg gcggcgtggt cgacgtcgtc gaacacgaaa
     3841 gatgtcgtct gatagatcgg tgtgacgcgc gcgcctgttg ttggatccgg ctgagccccg
     3901 gcatgaatgg caagcgtgtt gaacccgggc gtacgctctt ctgacatctt cggtctccct
     3961 ctcaccggtg tttgacgaac actatagcaa tctatatcgc gctgtcggcc agttacgaag
     4021 atgggccttt agcgcggcca attgggtgtc cgcttgtcga ggaaggcgcc gatgccttct
     4081 tgcgactccg caccgagcat gttcgccacc atgacgtcgg ccgtataacg gtaagcctct
     4141 tccagcgtca tctcggcttg tgtgtagaat gcctccttgc cggtcttcac cgtacgcgcg
     4201 ggcttctctg caatcttccg gccaaacgcg atcgcttcat tcacgacctg atccgccggc
     4261 accacgcgat tgatgagtcc aagcgtcttt gcgtcctccg cctcgaccat gtcgccaaaa
     4321 agcaacatct ccatggcggc tttgcgcgag acattacggc tgagtgccac catgggcgtt
     4381 gaacaaaaga gcccgatatt gacgccgggc gtggcgaagc gcgcatcgtc cgcggccacg
     4441 gcaagatcgc atgtggcgac gagctgacag cctgccgccg tggcaatgcc gttcaccgcg
     4501 gcaatgacgg gtttcggaca acgcacgatg gcgagcatca tgtcggaaca gacgcgcatg
     4561 gtgtccgcat aaaaactgcc gcccttgtcc gggtcgtcgc gatgggcggt gagctccttc
     4621 aggtcgtggc ccgaagaaaa ggcggggccg tgccccgcaa tcaccacggc gcggaccgcg
     4681 tccgccgcgc cggcgccgtc tatggcgcgg tgcagcgccg tcatcagatc catggagagg
     4741 caattgcgcg ctcccggccg gttcagggtc aggacaagga ccgggccttg ggcttcctgg
     4801 agcaggatgt cgtgctcgac catttcaccg tccaaaaggc ttttcgcgcg ctgccaaacg
     4861 gcttacagga aacgcctaaa aaggcacgcg aaatcagcag tgttgctagc ggtagcgcaa
     4921 taccacacac ttagcgcact gattatgcac cgaaaaccca attcgcttga ccacatcccg
     4981 cccagccgtt agaagcgccg atctttcgac cacccgaagt gatgcgagcc atgaaaacct
     5041 tttccgccac gcccaaggac atcgagaagt cctgggtcct catcgatgct agcggcctcg
     5101 tggtcggccg gcttgcggcg ctgattgcca cgcgcctgcg cggcaagcac aagcccggct
     5161 acacaccgca catggatgac ggcgacaata tcatcgtcat caacgcctcc gatgtggtct
     5221 tcacgggcaa caagcgcgcc aacaaggaat acttttggca caccgggtat ccgggcggca
     5281 tcaagagccg gaccgccgag aaggttctag agagcaaaaa tcctgagcgc gtgcttcaga
     5341 aggccgtgca gcgcatgctg cccgacggcc cgctggcgcg gaagcagctc aagaacttga
     5401 aagtctatgc cggctccgag catccccacg aggcgcagaa ccccgagaag ctcgacatcg
     5461 ccgccctcaa ttcgaagaac gcaaggagcg cttaagagcc atgagcgaga ccacgacgct
     5521 ggaagatctg gctcaactga agggcgaagg cgagggcgat gccccggcgc cggagcgtgt
     5581 ctacgaaaag aaggtggacg cgcaaggtcg cgcctatgcc acaggtaagc gcaaagacgc
     5641 cgtcgcgcgc gtctgggtca agcagggccc gggcaacatc atcgtgaacg gcaaggacca
     5701 cacggcttac ttcgcccgtc ccgtgctgca gatgattctg aagcagccgc tcgttaccgc
     5761 ggaccgcgag ggccagatcg acatcgtctg caccgtatcg ggtggtggac tgtcggggca
     5821 ggccggcgcc gtgcgtcacg gcatctccaa ggcgctgact catttcgagc ccgaactgcg
     5881 tagcgtgctc aagaaggcgg gctttctcac tcgcgatgcg cgcaccgtcg agcgtaagaa
     5941 gtacggtaag gcgaaggccc gacgaagctt ccagttctcc aagcgctagc gcttgtctct
     6001 ggaggttgcg ggagggggcc gaacagcctt tatcttccgc accgcgtcga attccgctaa
     6061 gcccgcatcc aacgcgggac gtcttcgtta gggtcgcaca tgaccagcaa tcagcctatc
     6121 cgggccgccg tgctcggcgc ctccggttac accggcgccg atctcattcg gcttgccgca
     6181 cggcacccga atattcaaat caccgcgctc atcgccaagg cccatgcggg ccagccgctt
     6241 ggccaagtct tcccgcatct cggtagcctc gatctgcccc agctcgtcac gtcggacgag
     6301 gtggattggg acagcatcga cgtcgcgttt tgcggcctgc ctcacggcac ggcccacgtc
     6361 gagattgcca aactgccgga gcgcgtgaag gtcatcgaca tgtccgccga ctttcgcttg
     6421 cgggatctgt cgacctacgc cgaatggtat ggcggcgaac acggcgcgcc tcagttgcta
     6481 agcgatgccg tctatgggct caccgagcac taccgcgacg agatcgcggg cgcccggatc
     6541 gtggcgtgtc cgggttgtta cccgaccgcg gtgctgctcg cgctcctgcc gcttgtgaaa
     6601 gagaaattga tcgcctcgga agggatcatc atcgatgcca agtccggcgt gactggcgca
     6661 ggacggtcct tgaaacagaa cattctgttc tccgaagcgg gcgaggggct gtcgccctac
     6721 gggatcggca gccatcgtca cgtgccggaa atcgagcagg aaatcggcat ggccgccaac
     6781 caagcggtga tcgtgaactt caccccgcat ctggcgccca tggcgcgcgg cgaactgtgc
     6841 acctgctatg tgcagctgcc gagcggcacg acggacgccg atttgcgcgg tgccttgcgg
     6901 gacgcctatg cggcaagccc cttcgtgcgg gtgatggacg agggcgtgat cccggccacg
     6961 cagcacgtgc gcgggtccaa ctactgccat gtcggcgtgt ttgccgaccg catccccggc
     7021 cgcgccatcg tcgtctcagc gatagacaac ttagtgaaag gctctgcggg tcaggccttg
     7081 cagaacttca acgtgatgca cggccttgac gagagcaccg cgttagagca actgccgctg
     7141 tttccgtgaa gccggcttag tcgcggtcct tcacgaaaga cccgggcgcg tcctcgatcg
     7201 gctttaaggc gccggccgac ggatcgcggg ccgggatctt ttcgccggat tgtccgctca
     7261 gccattcggc ccagtcgggc caccacgagc cgggggcctc ggctgccgtc ttggtccagg
     7321 cgtcgaacgt gtcagccggc gtaccggtcc agtattggta ctttttcttg gccggtggat
     7381 tgaccacgcc ggcgatatgg cccgagccgg ccatcacata gcgcaccggt ccgccgaaca
     7441 gcttgctgcc acgatagacc gacgttgccg gcgcgatgtg atcctctcgt gtgaacaact
     7501 cgtagatcgg gatcttcacc ttcgacaggt cgagcttcac accgccgagc tccatctcgc
     7561 ccttgctcaa acggttctga tgatagaact cgcgcaagta gaacgcgtgg ttcgctgccg
     7621 gcaaacgcgt ggagtccgcg ttccaataca gcagatcgaa cgggaagggc ttctttccga
     7681 gcagatagtt gttcacgatg tacggccaga tcaggtcgcg cggccgcagc atgttgaaca
     7741 cggcggccat gcgcgagccg tcgagatagc cttgctcggc catcatctcc tctaacgact
     7801 tgagctgtgc gtcgtcgatg aagacgagaa ggtcgcccgc ttcgctgaag tccacctgcg
     7861 cggtgaggaa agtcgcggtg gcgatacggt cgtcgccctt cgccgcagca taagcaaggg
     7921 tcgatgcgag cagcgtgccc ccgacgcagt agccgagcac attcacatgg tggagcccaa
     7981 gctgacggtc caccgcgtca acggccgcca gaatgccttc ggtcatgtag tcctcgaagg
     8041 tcttccgggc gagcgccgcc ttggggttca cccacgagat catgaacaca gtgaaaccct
     8101 ggtccaccgc gtactttacc agcgacttct ccggcttgag gtcgaggatg taatatttgt
     8161 tgatccacgg cggcgccata atcagcggcc gctcatagac ttgctcggtc gtcggagaat
     8221 attggatgag ctgaataagg tcgttctgga acacgacctt gccgggcgag atggcaatgt
     8281 cgcggccgac ctcgaaggcg gacaagtcgg tctggctgat gcgaagcaag tccttcgact
     8341 gttccagatc ctcggcgaag tgcgtcatgc cttggaccag gttctccgcg ttggactgaa
     8401 gcgtcgcgcg cacgacttcc ggattggtga agggattgtt cgacggcgac agcgcagcca
     8461 gcatctgttc caaatagaac agcgccttct tgcgagtctt gtcgtcgacg ccttcggtgt
     8521 tcttggtgac gtcctcggcc cagcgcgagg tgatgaggta agcctgcttc cagaaatcga
     8581 agaactggct ctccgaccac tccggatcct tgaagcgatt gtcgccgggc tccggctccg
     8641 ccaccggctc aacctcctcg ccaaagaaac gccgcacgga ccggccccac aggtcggcat
     8701 aattctggaa tagatcgcct tgcgcggccg ccagtttgtt ggggttggta acccaggcgc
     8761 ccgcgacttc gccgagcgtc ttggcggcct cggtgacctc ggaggccatg ctgtaggggc
     8821 caccattatt cccgttcgtg ctggagcgct cggccagtgt cgagaagact ttcgtgccct
     8881 gatcgaatag gcggaccata ttccgggcaa attcgtccat ttgggtcgtc tggctgtcgt
     8941 ccgcttccgg cttatcggcg ccgtccattc gctcctgcat caaaatgtcc ctgtctttgg
     9001 ccccaactga ccatgctccc gcctgtttgc agctcgaaaa ccttgggcag gttccgcata
     9061 gtcactcatt gtgctatgca atataagctt caggcgatgc ctatcgccag gggaaagtcc
     9121 acaagagcca acatcttacc gatgtttctg cgatcagcgt attgtctgat ggtcctactg
     9181 cctgcgcttc tgctcagcgg atgtgccggc agcatgcccg acaccagccg cctgaagcgg
     9241 tttaccgacg tgatccgggg ctatg
//
LOCUS       DBNHVU010000196         8342 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_444103, whole genome shotgun sequence.
ACCESSION   DBNHVU010000196 DBNHVU010000000
VERSION     DBNHVU010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 8342)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8342)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8342
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_444103"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="AC16Y7_08330"
     CDS             <1..293
                     /locus_tag="AC16Y7_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HIR5709426.1"
                     /translation="ILFLEQRAHGFDVWQEPFVQLRFPKLENLRMDPFERAEHEAIGY
                     GQWRAERMFALVPAQAFVSKWIASFKEFPPRQKAASFSVDQALEKLSDPKSR"
     gene            complement(381..800)
                     /locus_tag="AC16Y7_08335"
     CDS             complement(381..800)
                     /locus_tag="AC16Y7_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709427.1"
                     /translation="MRSMISKATLGLLCLFATPATVLAADGAFTCAFTDIFECADVVG
                     CKRVTLEFANLPPVLKIDFDKKVMTSDDIGRDAEEIELKNMVEEGDLVLLHGIGKNEV
                     SPRAFSAAISTKTGKLHAGISTGDATLSLVGDCVDGL"
     gene            complement(811..1257)
                     /locus_tag="AC16Y7_08340"
     CDS             complement(811..1257)
                     /locus_tag="AC16Y7_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709428.1"
                     /translation="MWIRTERKFMEQLRKFMCCAAIALVAVAGATYVHAQTGGRALLI
                     YKEGVTPEQEQKDRAACNEWAIQQSGFDPAAIYSAQQAGVRNRTIFEVVGKLDIASAG
                     TDPRWGSGAFGGASTSADKRRLAELYDSYLRAGALCLEGRGYAVTR"
     gene            complement(1328..1978)
                     /locus_tag="AC16Y7_08345"
     CDS             complement(1328..1978)
                     /locus_tag="AC16Y7_08345"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF036807.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family protein"
                     /protein_id="HIR5709429.1"
                     /translation="MSLKVIGAGFGRTGTLSLKRALEQLGFGPCYHMAETRHHPDHDA
                     AWLALARGETTDWHSVLTDYQATVDWPGVHFWKMLAAANPDAKIILTVRDPDRWYDSA
                     DQTIFRRMREFAKVLARGEDGTIWPPRYAHMRMVNAIVADDAFGGDLSRDHAVGVFNA
                     HNDDVCASVPAERLLVYRSGEGWDRLCGFLGVPIPQAPYPKVNSTADFNNQFPSDR"
     gene            complement(2002..2700)
                     /locus_tag="AC16Y7_08350"
     CDS             complement(2002..2700)
                     /locus_tag="AC16Y7_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR5709430.1"
                     /translation="MIEVRPFDTLGGANHGWLKAKHHFSFARYVDPNRMNHGALRVWN
                     DDEIKPNTGFPPHPHAEMEIITYVREGAITHEDSLGNTGRTEAGDVQVMSAGAGIRHA
                     EYNREDVPTTLFQIWIEPSAGGGTPSWGTKPFPRNERAGEFVALASGFEGDADALPIR
                     TDARVLGATLKAGDTAAYQLGPQRHAYLVPATGKIEVNGITADARDGVAIEDETAVTV
                     KAIEDADIVLVDAP"
     gene            2750..3730
                     /locus_tag="AC16Y7_08355"
     CDS             2750..3730
                     /locus_tag="AC16Y7_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010687246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR5709431.1"
                     /translation="MSLPGGSDGDGLRYAVVMKRYVRPCLEVVSGALVWLLLGAGAQG
                     ADVFEIRGVKAGVKAAGQAAAGPKPFVEIVCPLMQQQAEARGLPPMPFVRLIWRESRF
                     NPNAVSHKGAQGIAQFMPGTAADRGLADPFEPKSAITHSASLLADLRREFGNFGLAAA
                     AYNAGEERVRGWLAGKRTLPGETRRYVRFVTGRAAEDWQDPETDLPDTLKTEGSAVQD
                     SCKTLAPLVVRAVYETSPLTASGAWRPWGAHVSSAFSKAKALAKFGRLRRAHTSVLAQ
                     REPFVLPKRNFSRGRRALYMVQIGADSRKDARDICAALRKDGGACIVQKN"
     gene            3972..4295
                     /locus_tag="AC16Y7_08360"
     CDS             3972..4295
                     /locus_tag="AC16Y7_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709432.1"
                     /translation="MSRLMISGFIAAVFAAALFLSGPASAGPAGIAKPAQAENEMVKE
                     AGWRRRRWWRSNGYDTEVDAPTTSVRTNAANSSVDAPYTTVRKTRRGVWVRAPFVNLR
                     VPRRY"
     gene            complement(4353..5681)
                     /gene="chrA"
                     /locus_tag="AC16Y7_08365"
     CDS             complement(4353..5681)
                     /gene="chrA"
                     /locus_tag="AC16Y7_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HIR5709433.1"
                     /translation="MTAMSAVQTSPAENDPSFRDATAVWAKIGVISFGGPAGQIALMH
                     RTLVEERGWIGHERFLHALNFCMLLPGPEAMQLATYVGWRLHGTLGGLVAGLLFVLPG
                     AAVVLALSIAYALYGQVPLVEAAFVGIKAAVLVIVIEALVKVARRALHRPYDWVIAAA
                     AFIAIFFLDVPFPLIIAGAALAGYFLAFVDPVPVPEPLDMPPVPWTRTVRTAMLWLAI
                     WIVPLVAISAAFADTPVFADLSLFFSKLAVVTFGGAYAVLAYMAQQAVETYGWLSAGE
                     MLDGLGLAETTPGPLILVTEFVGFLGAYRNGGEPALAYGVLGVLVTLWATFAPCFLWI
                     FVGAPYVERLASNAKLAGALRGVTASVVGVILNLSLWFALHVIFERVEPEQHGIFRLW
                     TPDLASFDPTAALLALLAGILLLGFRLGVLTTLAITASAALLLAALSGST"
     gene            5783..7612
                     /locus_tag="AC16Y7_08370"
     CDS             5783..7612
                     /locus_tag="AC16Y7_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013947526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HIR5709434.1"
                     /translation="MTTAQLIQVTVFLAAAAIAAPLGRYSRMGAVLGYLAAGVVIGPY
                     VLGPLYALDDVEKVLHFGEFGVVLLLFVIGLELRPIRLWSMRASIFGLGAAQLVLTAA
                     ALAGVCLAFQVSFAQALFIGLALSLSSTAFALQVLEEKGELTTRHGRLAFSILLFQDL
                     AAIPLIALVPLFALGGEEPSMDLGAAGLAILTIIGVIVVGRFLLSRLYRLVAATGVRE
                     AMTASALLTVVGVALLMEEVGLSAALGSFIAGALLADSEYRHQIEADIAPFEGLLLAV
                     FFMAVGMSIDLSVLVARPGALLLIVAILIAVKFAVLYALGRWWGIGDRPARRLGIVLS
                     QGGEFAFVLFSVGLVEGVIGRDVAGLLMLAVTLSMATTPLLLLADDWLLRRKTADAPD
                     YELPPEAEKHVIIAGFGRFGQIVARVLRARQIPFTALDTSIAQVDFVKRFGAKIYYGD
                     AGRIDILRAAGADKANAFVLAIDNIDASLKVAEIVHENFPDLPIYARARDRIHVHKLM
                     DLGVTIIERETFRSALELTVQLLRGLGLKPAEAKRLVATFKQHDEKRLYDDYKDYTDS
                     EKMRANVLTAAVELEELFGRDIEDLENGGSASAPPRDGRESTG"
     gene            complement(7572..>8342)
                     /locus_tag="AC16Y7_08375"
     CDS             complement(7572..>8342)
                     /locus_tag="AC16Y7_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5709435.1"
                     /translation="LDEGKGPCALLGHCSSASHKEWLPLIEELKGDWRVLAPDFIGYG
                     QSDPWPIDKTFSIEADVKVLLALAKKTRAKIHLVAHSYGAALALEAARKLGSRVKSLT
                     LIEPVSFHLLRLENHPEWKDVEKLGISVLDPVAKGDDRKAAAAFMSYWLGRWRWWLSP
                     KRFKNAIATTIPKVALEFSIAIDAQTTLADYAEIEAPTLLIVGSKTPPPTRAVIELLS
                     DALPNARLETLKGAGHMSPFTHPAELNRLILDHLAAER"
BASE COUNT         1453 a         2537 c         2765 g         1587 t
ORIGIN      
        1 agattctgtt cttggagcaa cgcgcgcacg gcttcgacgt gtggcaggag cctttcgtgc
       61 aactccggtt cccgaagctc gaaaatctgc gcatggatcc gttcgagcgc gccgagcacg
      121 aagcgatcgg ctacgggcag tggcgtgcgg agcgcatgtt cgcacttgtg ccggcgcagg
      181 ccttcgtgtc gaagtggatc gcgagcttca aggagttccc accacgccag aaggccgcga
      241 gcttcagcgt cgaccaggct ctagagaaac tctccgatcc gaaatcgcgt tagcacgagg
      301 caaaataaat ggcgggaaga ttgctcttcc cgccattgtc tttacgccgt ctgacgtcga
      361 tcagttgcta gcgcagtgtc ttaaagaccg tccacgcagt cgccgaccaa agagagcgtc
      421 gcatcgccgg tcgaaatgcc ggcgtgaagc ttaccggtct tcgtggagat ggctgcgctg
      481 aaggctctag gcgaaacttc gttcttgcca atgccgtgca acaggacgag atcaccctcc
      541 tcgaccatgt tcttcagctc gatctcttcg gcgtctctgc cgatatcgtc cgatgtcatg
      601 accttcttgt cgaaatcgat tttgagtacg ggcgggagat tggcaaactc aagggtgacg
      661 cgtttgcagc ccaccacatc cgcacactca aagatatcgg tgaaggcaca cgtgaaggct
      721 ccgtcagctg ccaaaacggt cgccggcgtg gcaaacaggc agaggagccc gagggtagct
      781 ttagaaatca tcgatcgcat ggtttggctc ctatctcgtc acggcgtaac cgcggccttc
      841 aaggcaaagt gccccggccc ggaggtagct gtcgtaaagt tcggcgagac ggcgcttgtc
      901 ggcagacgtg ctcgcgcccc cgaacgcgcc ggacccccag cgcggatctg tgccggcgga
      961 cgcgatgtcc agcttcccaa cgacttcaaa gatcgtcctg ttcctcacgc cggcctgttg
     1021 cgccgagtag atggccgcag ggtcgaagcc gctttgttgg atggcccact cgttacaagc
     1081 tgcgcggtcc ttctgttcct gttccggcgt cacgccttcc ttgtagatca gcaacgcgcg
     1141 cccgccagtt tgtgcgtgga cataggtcgc gcctgccacg gccacgagcg ctatggctgc
     1201 gcagcacatg aactttctca actgctccat gaacttcctc tccgttctga tccacatggg
     1261 ggatcggccc cctaacatgc cgcagtccgg tgccagctgc aagcagccct cattttgccc
     1321 tggagggcta gcgatcgctc gggaattggt tgttgaagtc cgcggtgctg ttgaccttgg
     1381 ggtagggcgc ttggggaatg ggtaccccta agaagccgca aagccggtcc cagccctcgc
     1441 cggaccggta gacaaggagc cgttcggccg ggacgctggc gcacacgtcg tcgttgtggg
     1501 cattgaacac gccaaccgca tgatcgcggg agagatctcc accgaaggcg tcgtcggcca
     1561 cgatggcgtt gaccatgcgc atatgggcgt agcgcggggg ccagatcgtg ccgtcctcgc
     1621 cccgtgccag cactttcgcg aactctcgca tccggcggaa gatcgtctgg tccgcgctgt
     1681 cataccaacg gtccggatcg cgcacggtga ggatgatctt ggcgtccggg ttggccgcgg
     1741 ccagcatctt ccaaaagtga acgccgggcc aatcgacggt tgcttggtag tcggtcagca
     1801 ccgagtgcca gtccgtcgtc tcgccgcggg caagggcgag ccatgcggcg tcgtggtccg
     1861 gatggtggcg cgtctcggcc atgtggtagc agggaccgaa gccgagctgc tccagggcgc
     1921 gcttgagtga caaggtgccc gtgcgtccaa atcctgcgcc gatgactttt aaggacattg
     1981 cagccgaggc tccgcgagtg gctaaggcgc gtcgaccaga acgatgtcgg cgtcctcgat
     2041 ggctttcacc gtcaccgccg tctcgtcctc gatcgccacg ccgtcgcggg catctgccgt
     2101 gatgccgttg acctcgatct tgcccgtggc gggcacgaga taggcatgac gctgcgggcc
     2161 gagctggtac gcggctgtgt cgccggcctt tagcgtggcg ccgagaacgc gtgcgtcggt
     2221 gcggatcggc agtgcatccg catcgccttc aaatccggag gccaaggcga cgaattcgcc
     2281 cgcgcgttcg tttctcggga atggctttgt gccccatgac ggcgtgccgc cacccgcgct
     2341 cggctcgatc cagatttgga acaacgtcgt cggcacgtcc tcgcggttgt actccgcatg
     2401 gcggatgccg gcgcccgcgc tcatcacctg gacgtcgccc gcctcggtgc ggccggtgtt
     2461 accaaggctg tcctcatgcg tgatggcgcc ttcgcggaca taggtgatga tctccatctc
     2521 cgcgtgcgga tgaggcggga agccggtgtt cggcttgatc tcatcgtcgt tccagacgcg
     2581 caaagcgcca tggttcatgc ggttgggatc gacgtagcgg gcgaaggaga agtgatgctt
     2641 ggctttcagc cagccgtgat tcgcgccacc cagtgtgtcg aagggtctaa cctctatcat
     2701 caaagccctc ccgggggtca tttggctcca cgacatatag gaactcggtg tgtccctgcc
     2761 agggggcagt gacggggatg gattgcgcta tgctgtcgtc atgaagcgtt acgtccgccc
     2821 ctgtttggag gtcgtgtctg gcgcactcgt ctggctcttg ctcggtgcgg gtgcgcaagg
     2881 cgccgacgtc ttcgagatca ggggcgtcaa ggcgggggtc aaggccgcag gtcaagcggc
     2941 tgcgggaccg aagccgtttg tcgagattgt ttgcccgctg atgcagcagc aggccgaagc
     3001 gcgcggcctt ccgcccatgc cgttcgtgcg cctgatttgg cgcgagagcc ggttcaatcc
     3061 gaatgcggtt agtcacaagg gcgcgcaagg gattgcgcag ttcatgccgg ggacggcggc
     3121 cgatcgcggc ctggccgacc cgttcgagcc caaatccgca atcacgcatt cggcgagctt
     3181 gctggcggac cttcgcagag agttcggcaa tttcgggctc gcggcggccg cctataatgc
     3241 tggcgaggaa cgggtgcgcg gctggctcgc aggcaaacgc actttgccgg gcgagacgcg
     3301 gcgctatgtg cggttcgtta cggggcgcgc ggcagaagat tggcaagacc ccgaaacaga
     3361 tcttcccgat acgctcaaga cggaaggtag cgcggtgcag gactcctgca agacgctcgc
     3421 cccgctcgtg gtgcgcgccg tgtatgagac ctcgccgctc accgcgagcg gcgcctggcg
     3481 tccctggggg gcgcatgttt ccagcgcttt ctccaaggcc aaggcgctgg cgaagttcgg
     3541 caggctgcgg cgggcgcaca cttcggtcct ggcgcagcgc gagccatttg tgctgccaaa
     3601 gcggaatttc agccgtgggc gccgcgcgct ctatatggtg caaatcggcg ccgatagccg
     3661 gaaggatgcg cgcgacatct gcgcagcact ccgcaaggac ggcggggcct gcatcgtgca
     3721 gaagaattag gcggcgtact ggacatatgc gcctatgaat ccccaggtca tgccggagcg
     3781 cgacgggatc tttggctatc catttgcccc cgcgaccgat ggcgcttttt gccgtcttgc
     3841 cctaattatt cgttcaagca caaaggatgg ctctcgtttg gggttgcgcg gagtccgcgc
     3901 tgccgccaca ttgagcctgg aattccaagc tgcgattaga aatcagaccc tgaaccccaa
     3961 tggagcgaag gatgagccgt ctgatgattt ccggatttat tgccgccgtt tttgccgctg
     4021 ccttgttctt gtctgggccg gcctccgctg gtcctgcggg tatagccaag cctgcacagg
     4081 ccgagaacga gatggtgaaa gaggctggct ggcgccgccg ccgttggtgg cggagcaacg
     4141 gttacgacac cgaagtcgat gcgccgacca cgtcggttcg cacgaacgcc gcgaacagct
     4201 ctgtcgacgc tccgtacacg accgtgcgga agactcgccg gggcgtttgg gtccgtgcac
     4261 ccttcgtcaa tcttcgggtg ccgcgccgct attagcgtcc aggcgtattg cagatttgcg
     4321 accgagcgcg gatgagaagc cgcgctcttc gtttatgtgc tgccggatag cgcggcgagg
     4381 agaagtgctg cactggcggt gatcgcgagc gttgtcagga cgccgaggcg gaaacccaaa
     4441 aggagtatgc ctgcgaggag ggcaagcaac gccgcggtcg ggtcgaagct cgcaaggtcg
     4501 ggcgtccaaa gacgaaagat cccgtgctgt tcgggctcca cgcgctcgaa gatcacatgc
     4561 agcgcgaacc ataggctcaa gttcaagatg acgccgacca cggaggccgt gacgccgcgc
     4621 aaagccccgg caagtttggc gttcgaggcg agacgctcca cgtaaggcgc gccgacgaag
     4681 atccacagga agcagggcgc gaaggtcgcc cagagcgtga caagtacgcc gagcacgcca
     4741 taggccagcg cgggctcgcc gccgttgcga taggcgccga gaaagccgac gaactccgtg
     4801 accagaatga gcgggccggg cgttgtttcg gcgaggccaa gcccgtccag catctcgccc
     4861 gcgctcagcc agccataggt ctcgaccgcc tgctgcgcca tataggcaag taccgcgtag
     4921 gcgccgccaa aagtcaccac ggcgagcttg gagaagaaca gcgacaaatc tgcgaacacc
     4981 ggtgtgtcgg cgaatgccgc gctgatggca acgagaggta cgatccagat cgcaagccag
     5041 agcatcgcgg tgcggaccgt gcgcgtccaa ggcaccgggg gcatatcgag cggctccggg
     5101 acgggcaccg gatcgacaaa cgccagaaaa tatcccgcga gcgccgcacc ggcgatgatc
     5161 agcgggaacg ggacatcgag aaagaaaatg gcgatgaagg cggccgcggc gatgacccag
     5221 tcatagggac ggtgcagcgc gcgtcgtgcg accttgacca aagcctcgat gacgatcacg
     5281 aggacggcgg ctttgatgcc gacaaaggcg gcctcgacga gcggcacttg gccgtatagc
     5341 gcgtaggcga tcgacagcgc cagaacgacg gccgccccgg gcagcacgaa gagaagcccc
     5401 gccacgaggc cgcccagcgt gccgtgcaga cgccagccca cataggtcgc cagctgcatg
     5461 gcctccggac cgggcagcag catgcagaaa ttgagcgcat gcaggaaccg ctcatggccg
     5521 atccagccgc gctcctcgac cagcgtgcga tgcatgaggg cgatttgccc ggccgggccg
     5581 ccgaaggaga tcaccccgat cttggcccag acggccgtag cgtctcgaaa cgagggatcg
     5641 ttttcggcgg ggctcgtttg gacggcgctc atggcggtca ctcctagagg ctcatgccgt
     5701 ccgcggcaat cgtgctctgc cgggttgcta gctgcccggc gaatcgcgtg atacccaaag
     5761 gggagcaagg tagagggcga gcatgacaac agcgcagctg attcaggtga cggtattctt
     5821 ggccgccgcc gccattgctg cgcctcttgg ccggtattcc cgcatgggtg ccgtgcttgg
     5881 ctatttggcg gcgggggtcg tcatcggtcc ctatgtgctc ggaccgcttt acgccctcga
     5941 cgacgtcgag aaggttctcc atttcggcga gttcggggtg gtgttgctct tgttcgtcat
     6001 cgggctcgag ctgcgcccga tacgtctttg gtcgatgcgc gcgtcgatct tcgggctcgg
     6061 cgcggcacaa ctcgttctca cggccgcggc gcttgccggg gtgtgtctcg cattccaagt
     6121 gagctttgcg caggccttgt tcatcgggct tgccttgtcc ctctcgtcga ccgcttttgc
     6181 gctccaggtc ctggaggaga agggcgagct caccacgcgg catggccggt tggcgttctc
     6241 aatcctgctg ttccaggacc tcgccgcgat tcctctgatc gcgctggtgc cgctcttcgc
     6301 gctcggcggg gaggagccca gtatggactt gggcgccgcg gggctcgcca tcctcaccat
     6361 cattggcgtc atcgtggtag gccgcttcct cttgagccgg ctttaccgcc tcgtcgccgc
     6421 cacgggcgtg cgcgaggcga tgacggcaag cgcgctgctc acggtggtgg gcgtggcgct
     6481 cctcatggag gaggtgggac tatccgcggc gctggggtcc ttcatcgccg gcgcgctgct
     6541 cgccgactcc gaatatcgcc atcagatcga agcggacatc gcgccgttcg aaggcttgct
     6601 gctcgccgtg ttcttcatgg cggtgggcat gtccatcgac ttgtcggtgc tagtcgcgcg
     6661 gcccggcgcg ttgcttctga tcgtcgccat tctgatcgcc gtgaagtttg ccgtgcttta
     6721 cgcgctcggc cgctggtggg ggatcggcga taggccggcg cggcgcctcg gcatcgtgct
     6781 cagccaaggc ggtgagttcg ccttcgtgtt gttctcggtg ggcctcgtcg aaggcgtgat
     6841 cgggcgggac gttgcggggc ttttgatgct cgcggtgacc ctgtccatgg cgacgacgcc
     6901 gctgctcctg cttgctgacg actggctgtt gcgcaggaag acggcggacg cccccgacta
     6961 cgaattgccg ccggaggccg agaagcacgt catcatcgcg ggctttgggc ggttcggtca
     7021 gatcgtcgcc cgcgtcttgc gcgcgcggca aatccccttc acggcgctcg atacgagcat
     7081 cgcgcaggtt gacttcgtga agcgattcgg cgcgaagatc tattatggcg atgcggggcg
     7141 tatcgacatc ttgcgcgcgg cgggtgcgga caaggcgaat gcgttcgtgc tcgccatcga
     7201 caacattgac gcctcgctca aggtcgccga gatcgtgcac gagaactttc cagaccttcc
     7261 catctacgcg cgcgcgcgtg accgcatcca tgtgcacaag ctcatggatc tcggcgtgac
     7321 cattatcgag cgtgagacgt tccgctcggc gctggaattg acggtgcaac tcttacgcgg
     7381 gcttgggctg aaaccggccg aggcaaagcg gctcgttgcg acgttcaagc agcacgacga
     7441 gaagcggctt tacgacgact acaaggacta cacggactca gagaagatgc gggccaatgt
     7501 gctgaccgcc gccgtggagc ttgaggagct gttcggcagg gatatcgagg atctggagaa
     7561 cggcgggtcc gctagcgctc cgccgcgaga tggtcgagaa tcaaccggtt gagttcggcc
     7621 ggatgcgtaa acgggctcat atgccccgcg ccctttagcg tctcgaggcg cgcgttcggc
     7681 agcgcgtcag acagtaattc gatcacggcg cgcgtgggcg gcggcgtttt cgaccccacg
     7741 atcagaagcg tgggtgcctc gatctccgca tagtcggcaa gcgtggtctg tgcatcgatg
     7801 gcgatagaaa attccagcgc gactttggga atggtggtgg cgatggcgtt cttgaatcgc
     7861 ttgggcgaga gccaccagcg ccagcggccg agccagtagc tcatgaacgc cgcggcggcc
     7921 ttgcggtcgt cgcccttggc cacgggatcg aggaccgaga tgccgagctt ttcgacgtct
     7981 ttccattcgg ggtggttttc gagccgtaaa agatggaagg atacgggttc gatcagcgtg
     8041 aggctcttca cccggctgcc cagtttgcgc gcggcctcga gcgcaagcgc cgcgccataa
     8101 gaatgagcca ccaggtgaat cttggctctg gtcttcttgg cgagagccag cagcaccttt
     8161 acgtcggcct cgatggagaa cgttttgtcg atgggccagg ggtcggattg gccatagccg
     8221 atgaagtccg gcgcgagcac gcgccagtca cccttcagct cttcgatcag cggtagccat
     8281 tccttgtggg aggcgctgga gcaatggccg agcagggcgc atggcccctt gccttcgtcg
     8341 ag
//
LOCUS       DBNHVU010000197        12015 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_518366, whole genome shotgun sequence.
ACCESSION   DBNHVU010000197 DBNHVU010000000
VERSION     DBNHVU010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 12015)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12015)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12015
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_518366"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="AC16Y7_08380"
     CDS             complement(<1..265)
                     /locus_tag="AC16Y7_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013217271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane-spanning protein YciB"
                     /protein_id="HIR5709436.1"
                     /translation="MTPRPEQKTPKKDSEQSVAAKLLIEIGPLLVFFGVNAAYGIFAG
                     TGAFMVATVVSLSLAWWLYHKVPVMPLVSAVIVMIFGGLTLYLQ"
     gene            complement(249..1217)
                     /gene="ftsY"
                     /locus_tag="AC16Y7_08385"
     CDS             complement(249..1217)
                     /gene="ftsY"
                     /locus_tag="AC16Y7_08385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR5709437.1"
                     /translation="MNDTPAQPEPKTGWFTQLKAGLSRTSNSLTENLSSVVTKRKLDD
                     ETLDELEEVLIKADLGVAMADRIREALSRGRYDKGLTDNDVHAVLADEVTRVLEPVAE
                     PLKLDETAKPHVILVVGVNGTGKTTTIGKLAHLFAREGKRVMLAAADTFRAAAIDQLK
                     IWGERVGAEVVAQDVGSDPAGVGYEALELAKTEGADVLLIDTAGRLQNKGDLMAELAK
                     IVRVLKKLDENAPHTVMLVLDATTGQNAISQVQTFQEIAGVTNLVVTKLDGSARGGIL
                     VAIAERFGLPINAIGVGEGIEDFEPFDAHDFARAIAGAGRENDAAA"
     gene            complement(1214..2473)
                     /gene="mtaB"
                     /locus_tag="AC16Y7_08390"
     CDS             complement(1214..2473)
                     /gene="mtaB"
                     /locus_tag="AC16Y7_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013950222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035598 - tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HIR5709438.1"
                     /translation="MSEVDIITFGCRLNAYESEVIREHANAAGVTDAVIVNTCAVTGE
                     AVRQARQAIRKARRTRPGAKIVVTGCAAQIDPDQFAQMDEVDRVVGNQEKLEAKTFRG
                     LGTGDTQRIAVNDIMSVTETAGHLIEGFGNRARAYVQIQNGCDHRCTFCIIPFGRGPS
                     RSVPAGEVVAQVRRLVENGYAEIVLTGVDITAYGADLPGAMTLGKLTRTVLKLVPELP
                     RLRLSSIDSVEADPDLIKAIAEEERLMPHLHLSLQAGDDLTLKRMKRRHARADSVSFC
                     EAMRRVRPDIVFGADIIAGFPTETDEMFANSLRLVDDCGLTFLHVFPFSAREGTPAAR
                     MPQVNGTVVAERARALRQKGVAALKSHLESAKGRHIQVLMETEQQGRSPDFTPVRLDP
                     CAASAGALVDAVVSGDDGGALLAEAAQ"
     gene            complement(2505..3371)
                     /gene="dapF"
                     /locus_tag="AC16Y7_08395"
     CDS             complement(2505..3371)
                     /gene="dapF"
                     /locus_tag="AC16Y7_08395"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR5709439.1"
                     /translation="MDTNTRGFRKMNGLGNDFVVVDARSGALALSPEAVQAIADRKEG
                     IGCDQLIALEPSSQADVFMRIWNADGGEVAACGNAARCVAAVIAEEQGKPQVTIETED
                     QVLGAAVPGDGQVTIDMGAPRLRWDEIPLAEEFHDTRRIELQAGPIDAPVLHSPAVVN
                     VGNPHAIFFVDDVEAIDLARVGPMLEHHPLFPERANISVAQVLSPGHVKLRTWERGVG
                     LTRACGTAACASAVAAVRRELTDRNVKVSLPGGDLVIEWREADGHVLMTGPYALDFEG
                     TLPERLVAPAGV"
     gene            complement(3431..4624)
                     /locus_tag="AC16Y7_08400"
     CDS             complement(3431..4624)
                     /locus_tag="AC16Y7_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5709440.1"
                     /translation="MSAKATSRTRLPGTVWALGFTSLFMDISSELIHGLLPLFLVVTL
                     GASPIVLGVVEGIAEATAYIARVFSGWLSDTLQRRKALAVAGYGLAAITKPLFPIAHA
                     VWLVALARFLDRIGKGIRGAPRDALVADVTAPAQRGAAFGLRQSLDTVGATIGPLLAI
                     GLMLLFNDDIRTVLWFAVIPAALAVAILVFFVREPAPAERSARVPIRRDEIWKLGRFY
                     WFVVAAGAVFTLARFSEAFLIIRAHDAGLALALAPAVIAVMSLVFAVSAYPAGRLQDQ
                     FGAKPLLIAGLAALIAADLVLAFGQGLPAIFLGIGLWGLHMGLTQGVLSALVAEAAPA
                     RLRGTAFGIFGLITGLAALVASIAAGILWAVVSPTATFLVGAGFAAAALIAFAVLGAG
                     RKAAA"
     gene            4709..5908
                     /locus_tag="AC16Y7_08405"
     CDS             4709..5908
                     /locus_tag="AC16Y7_08405"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006169.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR5709441.1"
                     /translation="MVSVPGNDPVHALVPHTLEEPLMGANEGPLIGLTFMVKDLFAIK
                     GRKVSNGNPDFYEHASPASETAPAITRLLQAGASCTGITICDEFFYSVLGTNAHYGQP
                     VNARAGRYVTGGSSCGSAAAVAASMCDFALGSDTGGSIRVPASFSGLFGLRPTHGRIA
                     MGGVTPMAPSYDTVGVLARDAELFRNLGHVLLEGDRVDAPIERLILARDIVDYSEAST
                     DQLLWRTLDKLGDVIPKPVHADIAGETIEAWRKAFATIQGFEIQSTLLPFVQSHNVEL
                     GPGINERFEIAANVTFEEAEAARGVRTQVAEHLRSIVTPGTAIVLPTTPTLPPERDIP
                     DGASFAEFRTVTLRSTCSAGHAGLPQVSVPMGEASGCPVGLSFIGWQGGDEALLDLAA
                     KLSPHLT"
     gene            6194..6616
                     /locus_tag="AC16Y7_08410"
     CDS             6194..6616
                     /locus_tag="AC16Y7_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR5709442.1"
                     /translation="MDGTRKLRLAGILATLACLCLPLAAVGQQSHIPVIAGQDGPDYD
                     ACGSQGVVRGLDPKGDGFLAVRAGPSSQYAMVDKLNNGTVLNICDQSGKGGNWLGVVY
                     SHETMDCAVGTPWPRAGAYNGPCLSGWVYRKFVADLAG"
     gene            6616..8115
                     /gene="ffh"
                     /locus_tag="AC16Y7_08415"
     CDS             6616..8115
                     /gene="ffh"
                     /locus_tag="AC16Y7_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR5709443.1"
                     /translation="MFETLSDRLSGILDKLTRRGALSENDVSEAMREVRRALIEADVA
                     LDVVRSFTDRVKQKAIGQDVVKSVTPGQMVIKIVNDELVRMLGSDAQGIDLAATPPVT
                     MMLVGLQGSGKTTTTAKLAHRLTTRDKQKVMMASLDTRRPAAQEQLRVLGEQADVATL
                     PIVEGQSPTEIATRAIKAAKLGGFDVLLLDTAGRTHIDEELMAETAAIEKIAQPHETL
                     LVADALTGQDAANLARNFGERVDLTGIVLTRVDGDARGGAALSMRAVSGKPIKLIGIG
                     EKIDALEEFHPDRIAGRILGMGDVVGLVEKAIETVEIDKAEAIAKKVKKGAFDLEDLA
                     EQLKQMQKMGGMGGVLSMLPGIGKVKKQLDAANLDESIFNRQQAIISSMTRAERKTPK
                     LLNASRKKRIAAGSGTSVQDINKLVKMHRQMADMMKAMGKKRGALSALFGGGGAPEMP
                     ADMPQGGELPANFPGLPSGGLPGGLPPGLPGLPGNLPPGLPGLPKKKKR"
     gene            8152..8694
                     /gene="rpsP"
                     /locus_tag="AC16Y7_08420"
     CDS             8152..8694
                     /gene="rpsP"
                     /locus_tag="AC16Y7_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009464256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR5709444.1"
                     /translation="MSLKIRLSRAGAKKRPFYRIVIADSRSPRDGRYIERIGTFNPLL
                     PKDSDERVNLDTDRVKHWLSHGALPTDRVLRFLDAEGIMKREPRNNPQKAKPGQKRLD
                     REEEKAKAVEAAKAAKEEEKAAAAEAEKAAAETAADDAAAAEEEASVEAVDKTAAEEA
                     AEAEAPAKDAPAEDEAEKSE"
     gene            8716..9255
                     /gene="rimM"
                     /locus_tag="AC16Y7_08425"
     CDS             8716..9255
                     /gene="rimM"
                     /locus_tag="AC16Y7_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611583.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR5709445.1"
                     /translation="MGDNSERVLLGEIGAAQGLKGEVRLRSYTATPADIAAYGPLQDE
                     TGATSIEIERARVTPKALIVRIKGVSTREAAEALTGTKLYVPRTRLPEHGTDEWYVAD
                     LIGLEAVGKDGTVLGSVVAVHNFGASDIVEVAPASGGENLLVAFTDETVPEVDIENGR
                     LTLVPPRVLEDEPENGGEE"
     gene            9260..9964
                     /gene="trmD"
                     /locus_tag="AC16Y7_08430"
     CDS             9260..9964
                     /gene="trmD"
                     /locus_tag="AC16Y7_08430"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR5709446.1"
                     /translation="MTWRATVLTLFPEMFPGPLGLSLAGKALGDGKWALQTSDIRDFA
                     TDKHRSVDDTPAGGGAGMVMRADIAAAAIDAGRADAAPGTPAIYLSPRGAPLTQARAH
                     ELAQGPGVLLLCGRFEGVDQRVLEARDIEEISIGDYVLSGGELAAQVLIDAVVRLLPG
                     VAGNEGSLTEESFAAGLLEYPHYTKPREWEGRAIPEVLLSGDHEKIAEWRRKQAEALT
                     KARRPDLWAKRGKSQS"
     gene            10025..10345
                     /locus_tag="AC16Y7_08435"
     CDS             10025..10345
                     /locus_tag="AC16Y7_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5709447.1"
                     /translation="MKAMTLLAAAMAAGLPQGAAAQDADIAAGKAYAETICAACHAIL
                     PDEEVSPLLAATPFQEVANTRGMTELALTVWLQSSHPTMPNIILEQDDMRNVVAYIRS
                     LKGG"
     gene            10540..11238
                     /gene="rplS"
                     /locus_tag="AC16Y7_08440"
     CDS             10540..11238
                     /gene="rplS"
                     /locus_tag="AC16Y7_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR5709448.1"
                     /translation="MDIIAQLEKEQVAERLDKRSVPEFGPGDTVRVNVKVVEGERSRI
                     QAYEGVCIGRAGGGIHENFTVRKISYGEGVERVFPIHSPLIDSIEVVRHGRVRRAKLY
                     YLRGRRGKAARIPERKDVRAKKQKGKAFKGFTRPKGQPDDLKLIAGITEDLEKKLNKL
                     GLTKFEQIANLTDDEIAQVDDALSLGDKIEKDDWSAKAVALMAETTADEVPAADEGEA
                     APAEAEAEKSEKQD"
     gene            11409..>12015
                     /locus_tag="AC16Y7_08445"
     CDS             11409..>12015
                     /locus_tag="AC16Y7_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HIR5709449.1"
                     /translation="MAKPRTLYDKIWDEHLVHEAPDGTSLIYIDRHLVHEVTSPQAFE
                     GLRIAGRNVRSPEKTLAVVDHNVPTTDRSKGIDDPESKIQIDALAQNAEDFGIDYYHE
                     LDKRQGIVHVVGPEQGFTLPGTTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTL
                     IQKKAKNMLVRVDGKLPEGVTAKDIILAIIGEIGTAGGTGHV"
BASE COUNT         2303 a         4002 c         3830 g         1880 t
ORIGIN      
        1 cctgcaaata gagggtgagc ccgccgaaga tcatgacgat gacggcgctg accagcggca
       61 tcaccggcac cttgtgatac agccaccacg ccaaactcag agacaccacc gtcgccacca
      121 tgaaggcgcc ggtgccggcg aagatgccat aggcggcgtt cacaccgaaa aagacgagca
      181 gcggcccgat ctcgatgagg agcttggcag cgacggactg ctctgagtcc ttcttaggtg
      241 ttttttgttc aggccgcggc gtcattctcg cgtcctgctc cggcgatggc acgagcgaag
      301 tcgtgcgcgt cgaacggctc gaagtcctcg attccctcgc ctacgccaat ggcattgatg
      361 ggcagcccga agcgttcggc aatcgccacc aagatgccgc cacgcgcgct gccgtcgagc
      421 ttggtgacga caagattggt cacgccggcg atctcctgaa atgtctgcac ctggctgatc
      481 gcgttctggc cagtggtggc gtcgagcacg agcatcaccg tgtgcggtgc gttctcgtcg
      541 agcttcttca agacgcgcac gatcttggcg agttccgcca tgaggtcgcc cttgttctgc
      601 aagcgcccgg cggtgtcgat cagcagcacg tctgcgcctt cggtcttcgc gagctccaag
      661 gcctcgtagc caacgcccgc cgggtcggat ccgacgtcct gcgcgaccac ttcggcaccg
      721 acgcgctcgc cccagatctt cagctgatcg atggcggccg cacgaaatgt atccgccgcg
      781 gctagcatca cacgcttgcc ctctcgcgcg aatagatgcg caagcttgcc gattgtcgtg
      841 gtcttgccgg tgccgttgac gccgaccacc aggatcacat gcggcttggc ggtctcatcc
      901 agcttcaaag gttccgcgac cggctccagc acgcgcgtca cctcgtccgc cagcaccgcg
      961 tgcacgtcgt tatcggtaag ccccttgtcg tagcggccgc gcgacagcgc ctcgcgaatc
     1021 cgatcggcca tggcgacgcc gaggtcggcc ttgatgagca cctcttcgag ctcatccagc
     1081 gtctcgtcgt ccagcttacg cttggtgacg acactcgaga gattttcggt cagcgagttc
     1141 gaggtgcgcg aaagccccgc cttaagctgg gtgaaccagc ccgttttagg ctccggctgc
     1201 gccggggtgt cattcactgg gcagcctccg caagcagcgc cccgccgtca tcgcccgaga
     1261 cgaccgcatc gacgagcgcg ccggcgcttg cagcacaagg atcgagccgc acgggcgtga
     1321 agtccggcga gcgcccctgt tgctccgtct ccataagcac ctggatgtgc cggcccttgg
     1381 cgctttcaag atgcgacttc agggccgcaa cgcccttttg acgaagtgcg cgggcacgtt
     1441 cggcgacgac agtgccgttc acctgcggca tgcgcgcggc gggcgtaccc tcccgcgcgg
     1501 agaacggaaa cacgtggagg aaggtcagtc cgcaatcgtc cacgaggcga agcgagttgg
     1561 cgaacatctc gtccgtctcg gtgggaaaac cggcgatgat gtccgcaccg aacacaatgt
     1621 cgggccgcac acggcgcatg gcctcgcaga acgacacgga gtcggcgcgc gcgtgacgcc
     1681 gcttcatgcg cttcaaggtg aggtcgtcgc ctgcctgcag cgacaaatgc agatgcggca
     1741 tcagccgctc ttcttcggcg atagccttga tgagatcggg atcggcctcg acggaatcga
     1801 tggacgacag gcgcaagcgc ggcagctccg gcacaagctt caacaccgtg cgcgtgagtt
     1861 tgccgagcgt catggcgccg ggaaggtccg cgccataggc tgtgatgtcg acgcccgtca
     1921 gcacgatttc cgcgtagccg ttttcgacga gacgccgcac ttgcgcgaca acttcacccg
     1981 ccggcacaga gcgcgagggt ccgcgcccga agggaatgat gcaaaacgtg cagcgatgat
     2041 cgcagccatt ctgaatctgc acataggccc gcgcgcgatt gccgaagccc tcgatgagat
     2101 gaccggcagt ctccgtcacg gacattatgt cgttgacggc gatcctttgg gtgtcgccgg
     2161 ttccgagacc gcggaacgtc ttcgcctcga gcttctcctg attgccgacg acgcggtcca
     2221 cctcgtccat ttgcgcgaac tgatccggat cgatctgcgc cgcgcatccc gtaacgacaa
     2281 tcttggcgcc gggacgcgtg cgtcgcgcct ttcggatcgc ttgccgcgct tgtcgcacgg
     2341 cctcgcccgt caccgcgcaa gtgttgacga tcaccgcgtc ggtaacccct gcggcgttcg
     2401 catgctcgcg gatgacttcg gactcataag cgttcagccg gcaaccgaag gtgatgatgt
     2461 cgacctcaga catgagcggc ttctaacacg aaatccaaga tgcgtcagac cccggcaggc
     2521 gcgaccaagc gctccggcag ggtcccctcg aagtcgaggg cgtagggccc ggtcatcaac
     2581 acatggccgt ccgcctcgcg ccattcgatc acgagatcgc cgcccgggag cgagaccttg
     2641 acgtttcggt ccgtcagctc gcggcggacc gccgcaaccg cagaggcgca ggcggccgtg
     2701 ccgcaggcgc gcgtgagccc cacaccccgt tcccaggttc gcagtttcac gtggcccggg
     2761 ctcagcacct gggccacgga gatgttcgcg cgctccggga atagcgggtg atgctccagc
     2821 atcggcccga cccgggcgag atcgatggcc tcaacgtcgt cgacgaagaa gatggcgtgc
     2881 ggattgccga cattgaccac ggcgggagaa tgcagcaccg gcgcatcgat cggcccggcc
     2941 tgaagctcga tgcggcgtgt gtcgtggaac tcctcggcga gcgggatctc gtcccagcga
     3001 agacgtggcg cccccatatc gatggtcacc tggccgtcgc ctgggaccgc agcgccgagc
     3061 acctggtctt ccgtctcgat ggtgacctgc ggcttgccct gctcctcggc gatcaccgcc
     3121 gccacgcagc gcgcggcgtt cccgcaggcc gccacttcgc cgccatcggc gttccagatc
     3181 cgcatgaaca cgtcggcttg gctggacggc tccagcgcga tgagctgatc gcagccaatg
     3241 ccctccttgc ggtccgcaat ggcctggacg gcctccggcg aaagcgcgag cgcgcctgac
     3301 cgcgcgtcga ccacgacgaa gtcgttgccg agcccgttca tcttgcgaaa gccgcgcgtg
     3361 ttcgtatcca tgaccaaatc caattcctgc cgcctctata gcaccggcgc ggcgggagcg
     3421 cctccacgcc tcatgcggcg gccttgcgtc ccgcgccaag gactgcaaag gcgatcagcg
     3481 cggcggccgc aaatcctgcg cccaccagaa acgtcgccgt tggcgatacc actgcccata
     3541 ggattccggc ggcgatgctg gccaccagcg cggccagccc cgtgatcagg ccgaaaattc
     3601 caaacgccgt accgcgtagc cgcgccgggg cggcttcagc gacgagggcg gagagcacgc
     3661 cttgcgtgag ccccatatgg agaccccaca agccgatgcc aaggaagatc gccggcagtc
     3721 cctgcccaaa ggcgagcaca aggtccgcag cgatcagcgc ggcaaggccg gcgatcagca
     3781 aaggctttgc cccgaactgg tcctggagac gccccgccgg ataggcactc accgcaaaga
     3841 cgaggctcat caccgcaatg accgcaggcg ccagcgccag cgccagcccg gcgtcatggg
     3901 cgcggatgat gagaaaggct tccgagaacc gcgccagggt gaacaccgcg cccgccgcca
     3961 ccacgaacca atagaagcgg ccgagcttcc agatctcgtc ccgtctgatg ggaacgcgcg
     4021 cgctccgttc ggccggcgcc ggctctctga cgaagaagac caggatggcg acggcgagcg
     4081 cagccgggat caccgcaaac cacagcaccg tgcggatatc gtcgttgaag aggagcatca
     4141 gtccgatggc gagcagcggg ccgatggttg cgcccaccgt atcgagcgac tggcgcaggc
     4201 cgaatgccgc accgcgctgc gcaggcgccg tcacgtcggc caccagcgca tcgcgcggcg
     4261 ccccgcgaat gcctttgccg atgcggtcga ggaaccgcgc cagcgccacg agccagaccg
     4321 catgggcgat cgggaacagc ggcttggtga tggcggcgag cccgtagccg gcgacggcga
     4381 gggccttcct ccgctgcagc gtgtcggaca gccagccgga gaacacccgc gcgatatagg
     4441 cggtcgcctc ggcaatgccc tccaccacgc cgagcacgat ggggctcgcg cccaaggtca
     4501 ccaccagaaa tagcggcagc agtccatgga tcagctccga ggagatatcc atgaacagcg
     4561 aggtgaagcc gagcgcccag accgttcccg gcaagcgcgt gcgcgatgtg gccttggcac
     4621 tcaatgaagg gcctgctccg aagcgttgcg aagtgctcac ttatatggcg ttctgtccct
     4681 tagaacagtt ccgtaaaagg agaggcccat ggtctccgtt ccaggcaacg acccggtcca
     4741 cgctctcgtc ccccatacgc tggaagaacc gcttatgggc gcgaacgagg gcccgctcat
     4801 agggctcacc ttcatggtga aagacctgtt tgccatcaag ggacggaagg tttccaacgg
     4861 aaacccggac ttctacgagc atgccagccc ggcgagcgaa acggcgccgg cgatcacgcg
     4921 gctgcttcag gccggcgcgt cgtgcacggg catcaccatt tgcgatgagt tcttctacag
     4981 cgtgctcggc accaacgccc attacggcca gccggtgaac gcgcgcgcgg gccgctatgt
     5041 gaccggcggg tcgtcctgcg ggtccgcggc cgctgtcgcc gcctccatgt gcgacttcgc
     5101 gctcggctcc gacacgggcg gatcgatccg cgtgcccgcc tcgttcagcg gcttgtttgg
     5161 cttgcgtcca acacatgggc gcatcgccat gggaggtgtg acgcccatgg cgccgagcta
     5221 tgacacggtt ggcgttctcg cccgtgacgc ggagctgttt cgaaatctcg gccacgtcct
     5281 gctggaaggt gaccgcgtcg atgcacccat cgagcggctg atccttgccc gggatatcgt
     5341 cgattactcc gaggccagca ccgaccagct cctgtggcgg acactcgata agcttggaga
     5401 cgtgatcccc aaacccgtcc acgcggacat tgcgggcgag accatcgagg cctggcgcaa
     5461 ggccttcgcc acgattcaag gcttcgagat ccagtcgacg ctgctcccct tcgtgcagtc
     5521 gcacaatgtc gagctcgggc ccggcattaa cgagcgcttc gaaatcgccg cgaacgtcac
     5581 cttcgaggag gcggaggcgg cgcgcggcgt tcggacccaa gtggccgagc atttacgctc
     5641 catcgtgacg cccggcacgg ccatcgtgct gcccacaacg ccaacgcttc cgcccgaaag
     5701 ggacatcccc gacggcgcct ccttcgccga gttccgcacg gtgaccctgc gaagcacctg
     5761 ctcggccggc cacgccggcc tgccccaggt ctcggtgccc atgggcgagg cctcgggctg
     5821 ccccgtggga ctgtcgttca tcggctggca aggaggcgac gaggccctgc tcgaccttgc
     5881 cgccaagctc tccccgcact tgacttaagc gcgcctaagg acgacagtcc ggccgaaatt
     5941 caggcagctc cttaaccgag ccgccaccct gatcggggtc ctctcaaatg cgctcaagta
     6001 gcgcgaagcg cggtagcgca ccgagaagac taggcagggg ggtcaacacc cgtcagcgcg
     6061 atgcgcccac gggtgcgttt ttgctttgtg ctaccggcct ttggcctact tgagcgcagt
     6121 tttcgttgcg ctaccggcct ctggcctacc tgagcgcaga attgctttgc acgtcctctt
     6181 gggaggcgaa gacatggatg gaacacgcaa attgcgcctc gctggcatcc tcgccacgct
     6241 ggcatgtctc tgcctgcctt tggctgcggt gggccagcaa tcgcacatcc ccgtcatcgc
     6301 cgggcaagac ggcccggact acgacgcctg tggctcccaa ggcgtagtgc gcgggctcga
     6361 tccgaagggc gacggctttc tcgccgtgcg cgccgggccg agttcccaat acgccatggt
     6421 ggacaagctc aataacggca ccgtcctcaa catctgcgat cagagcggca aaggcggcaa
     6481 ttggctcggc gtcgtctatt cccacgagac catggactgc gccgtcggca cgccttggcc
     6541 gcgtgcgggc gcctataacg gtccatgcct gtccggctgg gtctatcgca aattcgtcgc
     6601 ggacctcgcc ggctgatgtt cgagaccctt tcagaccgcc tctccggcat tctcgacaag
     6661 ctcacgcgcc gcggcgcgct gagcgagaat gacgtctcgg aggccatgcg cgaggtgcgc
     6721 cgcgccctga tcgaggcgga cgtggccctc gacgtggtgc gctccttcac cgaccgggtg
     6781 aagcagaaag ccatcggcca ggatgtcgtt aaatcggtga cgcccggcca gatggtcatc
     6841 aagatcgtca atgacgagct ggtgcgcatg ctgggctcgg acgcacaagg catcgacctc
     6901 gccgccacgc cgcccgtcac gatgatgctg gtcggcctgc aaggctccgg taagaccacc
     6961 accaccgcca aactcgcaca ccggctgacc acgcgagaca agcaaaaggt catgatggcc
     7021 tcgctcgata cgcgccgtcc ggccgcgcag gagcagcttc gcgtgcttgg cgaacaagcc
     7081 gatgtcgcca cgctccccat cgtcgaaggc cagagcccga cggagatcgc gacgcgcgcc
     7141 atcaaagccg caaagctcgg cggcttcgac gtgctgctgc tcgatacggc cggccgcacg
     7201 catatcgacg aagagctgat ggccgagacg gccgccatcg aaaagatcgc gcagccgcac
     7261 gagacgctgc tcgtcgccga tgcgctgacc ggtcaggacg cggcgaacct cgccaggaac
     7321 ttcggcgagc gcgtggacct caccggcatc gtgctgaccc gcgtcgacgg cgatgcgcgc
     7381 ggcggcgctg cgctgtccat gcgcgcggtc tcgggcaagc ccatcaagct gattggcatc
     7441 ggcgagaaga tcgacgcgct cgaagagttt catcctgacc gcatcgcggg ccgcattctc
     7501 ggcatgggcg acgtggtcgg ccttgtcgag aaggccatcg agactgtcga gatcgacaag
     7561 gccgaggcca tcgccaagaa ggtcaagaag ggcgccttcg acctggaaga cctcgccgag
     7621 cagttgaagc agatgcagaa gatgggcggc atgggcggcg tgctctcaat gctgcccggc
     7681 atcggcaaag ttaagaagca gctcgacgcc gcgaaccttg atgaaagtat attcaaccgc
     7741 cagcaggcga tcatcagctc gatgacgcgc gccgaacgca agacgccaaa actgctgaac
     7801 gcctcgcgca agaaacgcat cgccgcgggc tccggtacga gcgtgcagga catcaacaag
     7861 ctggtgaaga tgcatcgcca gatggccgac atgatgaagg ccatgggcaa gaagcgcggc
     7921 gccctttccg cactgttcgg cggtggtggc gcacccgaga tgccggccga catgccccag
     7981 ggcggagagc tgccggcgaa ttttccaggc cttccatctg gaggtctgcc cggcggactg
     8041 ccgccagggc taccgggcct tccgggcaat ttgccgcccg gccttccggg cctgccgaag
     8101 aaaaagaaac gttgaaccct tttgtttgag atcacctagg agaaagaaac tatgtccctg
     8161 aagatcagac tgtcgcgggc cggcgccaag aagcgcccgt tctaccgcat cgtcattgcc
     8221 gactcgcgca gcccgcgcga tggccgctac atcgagagga tcggcacgtt caatccgctg
     8281 ctgccgaagg actccgacga gcgcgtcaac ctggacaccg atcgcgtgaa gcattggctg
     8341 tcgcacggcg cgctgccgac cgaccgcgtg ctccgcttcc tcgacgccga aggcatcatg
     8401 aagcgtgagc cgcgcaacaa cccgcaaaag gccaagccgg gccagaagcg cctcgatcgc
     8461 gaggaggaaa aggccaaggc agttgaggct gcgaaggccg cgaaggaaga agagaaggcc
     8521 gccgctgcag aggctgagaa ggctgctgcc gaaaccgcgg cagatgacgc cgccgctgcc
     8581 gaggaagaag cctcggtcga ggccgtagac aagaccgccg cggaagaggc tgccgaggct
     8641 gaagcgcctg cgaaggacgc tcctgccgag gacgaggccg agaagtccga gtagccttca
     8701 aaagaggtca cgaccttggg cgacaattcg gagcgggtgc tacttggcga aatcggcgcg
     8761 gcccaagggc tgaagggcga ggttcgcctg cgttcctata cggcaacgcc ggccgacatc
     8821 gccgcctacg gtccgctcca ggacgagacg ggtgcgacgt cgatcgagat cgagcgcgcc
     8881 cgcgttacgc ccaaagcgct catcgtccgc atcaaaggcg tgagtacgcg agaggccgcc
     8941 gaggcgctga ccggtacgaa gctctatgtg ccacgtacgc ggttgcctga gcacgggacg
     9001 gatgaatggt acgtcgccga cttgatcggc ctcgaagcag tgggcaagga cggcactgtg
     9061 ctcggcagcg tggtggcggt gcataacttc ggcgcgagcg acatcgtcga ggtcgcgccc
     9121 gcgagcggag gcgaaaacct cctcgtcgct ttcaccgacg agaccgtgcc ggaggtagac
     9181 attgagaatg ggcggttgac gctggtgccg ccaagggtcc ttgaggatga gccggagaac
     9241 ggcggcgagg aatgatccga tgacctggcg cgccacggtc ctcacgctct tccccgaaat
     9301 gtttccgggg ccgctgggct tgagcctcgc cggcaaggca cttggcgacg ggaaatgggc
     9361 gcttcagacc agcgatattc gcgacttcgc caccgacaag caccgctccg tggacgacac
     9421 gccggcgggc ggcggtgccg gcatggtgat gcgggccgac atcgctgcgg ccgccatcga
     9481 tgcgggccgg gccgatgccg cgcccggcac gcctgcgatc tacctgtcgc cgcgcggcgc
     9541 gccgctcacg caagcccgcg cgcacgaact cgcgcagggc cctggcgtgc tgctgctctg
     9601 cggccgcttc gagggcgtcg atcagcgcgt cttggaggcg cgcgacatcg aggagatctc
     9661 catcggcgac tatgtgctct ccggtggcga actcgcggcc caagttctga tcgacgcggt
     9721 cgtgcggctg ctgccgggtg tcgccggcaa cgaaggctcg ctcaccgagg agagcttcgc
     9781 ggcagggctt ttggagtacc cgcactacac caagccccgg gagtgggaag gccgggccat
     9841 ccccgaggtg ctgctctccg gcgaccacga aaaaatcgca gaatggcgac gaaaacaggc
     9901 cgaagccctg accaaggccc gaaggccgga tctttgggcc aagcgcggca aatcccaatc
     9961 ttgatcgaag tcagggcggc ggcgcctgca acgcccaaac tcgctgcaaa agaaggagtt
    10021 gggcatgaaa gcgatgacgc tgctcgctgc cgccatggcg gccgggctcc cccagggcgc
    10081 ggcggctcag gacgccgata tcgccgccgg gaaggcctat gccgagacga tctgcgccgc
    10141 ctgccacgca attctgccgg acgaggaggt ctcgccgctt ctcgcagcga cgcccttcca
    10201 ggaggtcgcc aacacaaggg gcatgaccga attggcgctc accgtctggc tgcagagctc
    10261 ccaccccacc atgcccaaca tcattttgga gcaggacgac atgcgaaacg tggtcgccta
    10321 tatccgcagc ctcaagggcg ggtgagcgtc cagcctgcct ccccttgcga ggggagcgat
    10381 tctgctagga aaatccatcg acaagggcga gaagctgggc taggaaagcc ccgctggttg
    10441 ctggattttg ccacgcaccc ggtactttcg atcaatcaaa aagaatggcg tacgtgaggg
    10501 gccaaaggcc tcggcaggcg cccgtgaaag gcataaaaga tggatattat cgcccagctc
    10561 gagaaagagc aggtcgccga gcgccttgat aagcgctccg tgccggagtt cggacccggc
    10621 gacaccgtcc gcgtcaacgt gaaagtcgta gaaggcgagc gctcgcgcat tcaggcctat
    10681 gaaggcgtat gcattggccg cgccggcggc ggcatccacg agaacttcac cgtccgcaag
    10741 atttcctacg gcgagggcgt ggagcgtgtg ttcccgatcc actcgccgct gatcgactcc
    10801 atcgaggtgg tgcgccacgg ccgtgtgcgc cgcgccaagc tctactacct gcgtggccgc
    10861 cgcggtaagg ccgcccgcat ccccgagcgc aaagacgtgc gcgccaagaa gcagaagggc
    10921 aaggccttca agggcttcac caggcccaag ggccaacctg acgatctgaa gctgatcgcc
    10981 ggcatcaccg aggacctgga gaagaagctc aacaagctgg ggctcaccaa gttcgagcag
    11041 atcgccaacc taaccgacga cgagatcgca caggtcgacg atgccctctc ccttggcgac
    11101 aagatcgaga aagacgactg gtcggcgaag gccgtcgcgc tgatggccga gaccacggcc
    11161 gacgaggttc ccgccgccga cgaaggcgaa gcggctccgg cggaagcgga agccgaaaag
    11221 tccgagaaac aggactagcg tttcatcgct gtcgtggccg ggcgtgtccc ggccatccac
    11281 gatttcccgc cgacaggccc caagacgtgg atgcccggca caagcccggg catgacgcat
    11341 ggttggcaag cggcccttca atcgctatct gacgcttacg acaacgtccg aaacgagacc
    11401 ctgaaaacat ggcgaagccc cgcaccctgt acgacaagat ctgggacgaa cacctggtcc
    11461 acgaggcgcc ggacggcacc agcctgatct atatcgaccg tcatctggtg catgaggtga
    11521 cgagcccgca ggccttcgag ggcctgcgca tagcgggccg caacgtgcgc agccccgaaa
    11581 agacgcttgc cgtggtcgat cacaacgtac cgacgacgga ccgctccaaa ggcatcgacg
    11641 atcctgaatc caagatccag atcgacgcgc tggcgcagaa cgcggaagat ttcggcatcg
    11701 actactacca cgagctcgac aagcgccagg gcatcgtcca cgtggtcggc cccgagcaag
    11761 gctttacgct tcccggcacc accatcgtgt gcggtgacag ccacacctcg acgcacggag
    11821 cctttggcgc cttggcgcac ggcatcggca cctccgaggt ggagcatgtg ctcgccaccc
    11881 agacgctgat ccagaagaaa gccaagaaca tgctggtgcg cgtcgacggc aaactgcccg
    11941 aaggcgtcac cgccaaggac atcatcctcg ccatcatcgg cgagatcggc acggctggcg
    12001 gcactgggca cgtca
//
LOCUS       DBNHVU010000198         2987 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Methyloceanibacter sp. isolate MAG05112
            SRR25158351_k127_221000, whole genome shotgun sequence.
ACCESSION   DBNHVU010000198 DBNHVU010000000
VERSION     DBNHVU010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414520
            Sequence Read Archive: SRR25158351
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methyloceanibacter sp. (soil metagenome)
  ORGANISM  Methyloceanibacter sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Hyphomicrobiales; Hyphomicrobiaceae; Methyloceanibacter.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 06:29:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,693
            CDSs (total)                      :: 1,670
            Genes (coding)                    :: 1,659
            CDSs (with protein)               :: 1,659
            Genes (RNA)                       :: 23
            tRNAs                             :: 19
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 11
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 2 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Methyloceanibacter sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158351_k127_221000"
                     /isolate="MAG05112"
                     /isolation_source="soil"
                     /db_xref="taxon:1965321"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="30.47 N 122.11 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="AC16Y7_08450"
     CDS             complement(<1..224)
                     /locus_tag="AC16Y7_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR5709450.1"
                     /translation="MAEGGQRLTTGMALPSVTLPATDGAEIDVAPLPGRSVIAVYPWT
                     GCPGQPNPPLWDDIPGAHGSTPELERFGALS"
     gene            complement(227..697)
                     /gene="smpB"
                     /locus_tag="AC16Y7_08455"
     CDS             complement(227..697)
                     /gene="smpB"
                     /locus_tag="AC16Y7_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR5709451.1"
                     /translation="MAPKRESGKLVAENRKARFNYAIEDTIEAGIVLSGSEVKSLRAG
                     KGNIAESYVSDEGGELFLINARIDEYKGAARDGHEPTRHRKLLLHKKEMARLSGAIQR
                     EGMALIPLRLYFNARGLAKLQLGLGRGKKIYDKRETQKKRDWERQKGRLMREKG"
     gene            complement(719..1597)
                     /gene="dapA"
                     /locus_tag="AC16Y7_08460"
     CDS             complement(719..1597)
                     /gene="dapA"
                     /locus_tag="AC16Y7_08460"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR5709452.1"
                     /translation="MFQGSITALITPFKDGSLDETAFRKLVEWQIDQGTHGLVPCGTT
                     GESPTLDHDEHRRVIELCIDAADRRVPVIAGTGSNSTAEAVELTRHAKSAGADGALVV
                     TPYYNKPTQEGLYLHYKAINDATDIPIVIYNIPGRSVIDMSVDTMARLFKLKNIVGVK
                     DATANMARVSEQRAAMGHEFIQLSGEDATALGFMAHGGVGCISVTANVAPALCSEFQL
                     ACLGGNLKRALEIQDRLMPLHDALFVEANPGPVKYAAAKLGLCSGETRLPLAPIGDAA
                     RKSVDEAIAAVGLATV"
     gene            1973..>2987
                     /locus_tag="AC16Y7_08465"
     CDS             1973..>2987
                     /locus_tag="AC16Y7_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5709453.1"
                     /translation="MKRISAHYLILLVAMALAGSVPAMAKTDAVPLPDRNPERPAVAA
                     ADPAAPAQEAAQETPATPSETPTAAAPAMPASPQANEANTTTVAAIPKPERNPQNTTP
                     ISEPATEDAKADGDGAQDVKGEVAKAEDAKAEDAGETEEEASGPFVAPDNAPLPEINP
                     NRDPTFVKAPTPALDYAAILEPLLSYKISSDDESRMRFVMRNKGSVEETAAKISDPAV
                     RDFAFWYRYKHNKSTFLDAAAVEAYRLSHPMWPSQDKLREKAETSLLLKDAPPDQIKA
                     FFDAKPPQTGAGEAALATVYMKENREAEAKDLIASAWCDHRLHPDVEKKILDSFGSML
                     THKH"
BASE COUNT          573 a          974 c          908 g          532 t
ORIGIN      
        1 gagagggcgc cgaagcgctc caattcaggt gtcgagccat gggcgccggg aatatcgtcc
       61 caaagcggcg ggttcggctg accgggacac cccgtccagg gatagacggc gatgacgctg
      121 cgcccaggca gcggcgcgac gtcgatctca gcgccatcgg ttgccggcaa ggtcacagac
      181 ggtaaggcca tgcccgtggt caggcgctgg cctccctccg ccatgctcag cccttctccc
      241 gcatgagccg gcccttctgg cgctcccaat cgcgcttctt ctgcgtctcg cgcttgtcgt
      301 agatcttctt gccgcggccg agcccaagct ggagcttggc cagcccacgt gcattgaagt
      361 agaggcgtaa aggaatgagc gccatgcctt cgcgttggat tgcgcccgac aaccgcgcca
      421 tttccttctt atgcagcagg agttttcggt gccgcgtggg ctcgtggccg tcgcgcgcgg
      481 cgcccttata ctcgtcgatg cgggcgttga tcagaaagag ttcgccgcct tcgtcggaga
      541 cgtaggactc cgcgatgttc cccttgcccg cgcgcagcga cttcacctcc gagccgctga
      601 gcacaatacc ggcttcgatt gtatcttcga tcgcgtagtt gaaacgagcc ttgcggttct
      661 cggcgacgag ctttccgctc tcgcgcttcg gcgccatgat gagcctcttt ccgcgaaact
      721 agaccgtggc gaggccaacg gccgcaatcg cctcgtccac gctttttcgc gccgcatcgc
      781 cgatcggcgc cagcggcagc cgcgtctcgc cactgcatag accaagcttg gcggcggcat
      841 atttcaccgg tccgggattg gcttccacga acagtgcgtc atgcagcggc atgagccggt
      901 cctggatctc gagcgcccgc ttcaggtttc cgccaaggca ggcaagttga aactccgaac
      961 agagcgccgg tgccacattg gcggtcaccg agatgcaccc caccccgcca tgggccataa
     1021 agccaagcgc cgttgcgtcc tcgcctgaga gctggatgaa ctcatgcccc attgccgcgc
     1081 gctgttcact gacgcgcgcc atgttggccg tcgcatcctt cacgcccacg atgttcttga
     1141 gcttaaacag ccgcgccatc gtgtccaccg acatgtcgat cacgctgcgg cccgggatgt
     1201 tgtagatgac gatgggaatg tcggtcgcat cgttgatggc cttgtagtgg aggtaaagcc
     1261 cctcctgggt cggcttgtta taatacggcg tcaccacgag cgcgccgtcg gcgccggcgg
     1321 acttcgcgtg ccgcgtgagt tccaccgcct cagccgtcga gttggagcct gtcccggcga
     1381 tcacgggcac ccggcgatcg gccgcgtcga tgcacagctc gataacgcgc cgatgctcgt
     1441 cgtgatcgag cgtgggggat tcgcccgtgg tcccgcacgg cacgagcccg tgggtgcctt
     1501 ggtcgatctg ccactccaca agcttccgga aggcggtctc gtccagagag ccatccttga
     1561 agggcgtgat gagggccgtg atcgagcctt gaaacatcgg catggtcaaa gcaggttcct
     1621 actcggagcg cgtgttcttg aaaaacgtac gaaaagtggg gcggacaata gggtctgcgg
     1681 cgtgaggcgg caagcaggcg cgcggacgga gcgcatggct tcaagctgat tgcggcccag
     1741 gcgcccctag ctcaccagca gactttcaaa attatacgcc tgattagcgg gttatgggcg
     1801 ctatttcagg tcctatctga cgtggcgccg acgcccagca gtcatttttc cgccggaatc
     1861 tttgagacat tctgggaatt ggatacactt atgtgacctt gatcgtcgcg gctcccggtc
     1921 gggggacgta ctgaccgcgc gatacaggcc gtaaccggta ccgtccgacg agatgaaacg
     1981 catcagcgcg cactatttga tattgcttgt cgcgatggcc ctggccggct ctgtgcccgc
     2041 catggcgaag acggatgccg ttcccctgcc cgatcggaac ccggagcgtc cggcggtggc
     2101 cgccgccgac ccggcagccc cggcacaaga ggccgctcaa gagacgcccg ccaccccgtc
     2161 cgagacgccg accgccgctg caccggcaat gcccgcctcg ccacaagcca acgaggccaa
     2221 cacgacgact gttgccgcca tcccgaaacc tgagcgcaat cctcagaaca ctacgccaat
     2281 ctccgagccg gcgacggaag acgcaaaggc cgacggcgac ggcgcgcagg acgtaaaagg
     2341 ggaagtcgcg aaggcggaag acgcaaaagc ggaagacgcg ggcgaaaccg aagaagaagc
     2401 cagcggcccc ttcgttgcgc ccgacaacgc accacttccc gagatcaatc ccaaccggga
     2461 tccgaccttc gtgaaggcgc cgacaccggc tctggactac gccgcgatcc tggagccgct
     2521 gctgtcctac aagattagtt ccgacgatga gagccggatg cgcttcgtca tgcgcaacaa
     2581 gggttccgtc gaagagacgg ccgcgaagat ttccgatccc gcggtgcgcg acttcgcgtt
     2641 ctggtatcgc tacaagcaca acaagagcac gtttttggac gcggccgccg tcgaggccta
     2701 ccgcttgagc catcccatgt ggcccagcca agacaagctg cgcgaaaagg ctgagacctc
     2761 gctcttgctc aaggacgccc cgccagatca gatcaaagcc ttcttcgatg ccaagccgcc
     2821 gcaaacaggc gccggtgagg cggcgctggc gactgtctac atgaaggaga accgagaggc
     2881 cgaagccaag gacctcatcg cctcggcatg gtgcgatcat cggctccacc cagacgttga
     2941 aaaaaagatc ctcgatagtt ttggatcgat gctcacgcac aagcatc
//
